LOCUS       DAEMHZ010000001        99132 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000001 DAEMHZ010000000
VERSION     DAEMHZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 99132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.001"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..203
                     /locus_tag="KPK72_000001"
     CDS             <1..203
                     /locus_tag="KPK72_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487258.1"
                     /translation="KERGMTQNELAEKANYSRAYISDLENKRYKNINISTIIDLSLAL
                     EIDFLELCRYYCEEELRRRKLM"
     gene            1034..2551
                     /locus_tag="KPK72_000002"
     CDS             1034..2551
                     /locus_tag="KPK72_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2487259.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVINKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            2574..2858
                     /locus_tag="KPK72_000003"
     CDS             2574..2858
                     /locus_tag="KPK72_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2487260.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(2955..4619)
                     /locus_tag="KPK72_000004"
     CDS             complement(2955..4619)
                     /locus_tag="KPK72_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG2487261.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(4691..5938)
                     /locus_tag="KPK72_000005"
     CDS             complement(4691..5938)
                     /locus_tag="KPK72_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2487262.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(5925..6599)
                     /locus_tag="KPK72_000006"
     CDS             complement(5925..6599)
                     /locus_tag="KPK72_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG2487263.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(6771..7850)
                     /gene="mltG"
                     /locus_tag="KPK72_000007"
     CDS             complement(6771..7850)
                     /gene="mltG"
                     /locus_tag="KPK72_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG2487264.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPNGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            complement(8063..9388)
                     /locus_tag="KPK72_000008"
     CDS             complement(8063..9388)
                     /locus_tag="KPK72_000008"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG2487265.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            complement(9592..10404)
                     /locus_tag="KPK72_000009"
     CDS             complement(9592..10404)
                     /locus_tag="KPK72_000009"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG2487266.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            complement(10451..11623)
                     /locus_tag="KPK72_000010"
     CDS             complement(10451..11623)
                     /locus_tag="KPK72_000010"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG2487267.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKDIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            complement(11665..12579)
                     /locus_tag="KPK72_000011"
     CDS             complement(11665..12579)
                     /locus_tag="KPK72_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2487268.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            complement(12591..13196)
                     /locus_tag="KPK72_000012"
     CDS             complement(12591..13196)
                     /locus_tag="KPK72_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG2487269.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            complement(13264..13800)
                     /locus_tag="KPK72_000013"
     CDS             complement(13264..13800)
                     /locus_tag="KPK72_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2487270.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            complement(13802..14869)
                     /locus_tag="KPK72_000014"
     CDS             complement(13802..14869)
                     /locus_tag="KPK72_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG2487271.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            complement(14874..15698)
                     /locus_tag="KPK72_000015"
     CDS             complement(14874..15698)
                     /locus_tag="KPK72_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2487272.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            complement(15706..16398)
                     /locus_tag="KPK72_000016"
     CDS             complement(15706..16398)
                     /locus_tag="KPK72_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2487273.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            complement(16422..17297)
                     /locus_tag="KPK72_000017"
     CDS             complement(16422..17297)
                     /locus_tag="KPK72_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG2487274.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            complement(17315..18436)
                     /locus_tag="KPK72_000018"
     CDS             complement(17315..18436)
                     /locus_tag="KPK72_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487275.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            complement(18849..19673)
                     /gene="spo0A"
                     /locus_tag="KPK72_000019"
     CDS             complement(18849..19673)
                     /gene="spo0A"
                     /locus_tag="KPK72_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG2487276.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            complement(19815..20873)
                     /locus_tag="KPK72_000020"
     CDS             complement(19815..20873)
                     /locus_tag="KPK72_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2487277.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            21034..21474
                     /locus_tag="KPK72_000021"
     CDS             21034..21474
                     /locus_tag="KPK72_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2487278.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            21535..22026
                     /locus_tag="KPK72_000022"
     CDS             21535..22026
                     /locus_tag="KPK72_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2487279.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22119..22985)
                     /locus_tag="KPK72_000023"
     CDS             complement(22119..22985)
                     /locus_tag="KPK72_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487280.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIKKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKFTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDGIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(23200..24879)
                     /gene="recN"
                     /locus_tag="KPK72_000024"
     CDS             complement(23200..24879)
                     /gene="recN"
                     /locus_tag="KPK72_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG2487281.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKKVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            complement(24899..25711)
                     /locus_tag="KPK72_000025"
     CDS             complement(24899..25711)
                     /locus_tag="KPK72_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG2487282.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            complement(25844..27709)
                     /locus_tag="KPK72_000026"
     CDS             complement(25844..27709)
                     /locus_tag="KPK72_000026"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG2487283.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            complement(27743..28180)
                     /locus_tag="KPK72_000027"
     CDS             complement(27743..28180)
                     /locus_tag="KPK72_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG2487284.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            complement(28210..29097)
                     /locus_tag="KPK72_000028"
     CDS             complement(28210..29097)
                     /locus_tag="KPK72_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG2487285.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEDGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            complement(29100..29315)
                     /gene="xseB"
                     /locus_tag="KPK72_000029"
     CDS             complement(29100..29315)
                     /gene="xseB"
                     /locus_tag="KPK72_000029"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG2487286.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            complement(29317..30522)
                     /locus_tag="KPK72_000030"
     CDS             complement(29317..30522)
                     /locus_tag="KPK72_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG2487287.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLENIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            complement(30519..31589)
                     /locus_tag="KPK72_000031"
     CDS             complement(30519..31589)
                     /locus_tag="KPK72_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG2487288.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMSKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            complement(31573..32079)
                     /gene="nusB"
                     /locus_tag="KPK72_000032"
     CDS             complement(31573..32079)
                     /gene="nusB"
                     /locus_tag="KPK72_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG2487289.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            complement(32164..32526)
                     /locus_tag="KPK72_000033"
     CDS             complement(32164..32526)
                     /locus_tag="KPK72_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2487290.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIRYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            complement(32636..33325)
                     /locus_tag="KPK72_000034"
     CDS             complement(32636..33325)
                     /locus_tag="KPK72_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG2487291.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            complement(33352..33906)
                     /locus_tag="KPK72_000035"
     CDS             complement(33352..33906)
                     /locus_tag="KPK72_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG2487292.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            complement(33918..34535)
                     /locus_tag="KPK72_000036"
     CDS             complement(33918..34535)
                     /locus_tag="KPK72_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG2487293.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            complement(34595..35800)
                     /gene="spoIIIAE"
                     /locus_tag="KPK72_000037"
     CDS             complement(34595..35800)
                     /gene="spoIIIAE"
                     /locus_tag="KPK72_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG2487294.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            complement(35885..36271)
                     /gene="spoIIIAD"
                     /locus_tag="KPK72_000038"
     CDS             complement(35885..36271)
                     /gene="spoIIIAD"
                     /locus_tag="KPK72_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG2487295.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            complement(36287..36484)
                     /gene="spoIIIAC"
                     /locus_tag="KPK72_000039"
     CDS             complement(36287..36484)
                     /gene="spoIIIAC"
                     /locus_tag="KPK72_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG2487296.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            complement(36501..37022)
                     /locus_tag="KPK72_000040"
     CDS             complement(36501..37022)
                     /locus_tag="KPK72_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG2487297.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            complement(37012..37980)
                     /gene="spoIIIAA"
                     /locus_tag="KPK72_000041"
     CDS             complement(37012..37980)
                     /gene="spoIIIAA"
                     /locus_tag="KPK72_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG2487298.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            complement(38204..40189)
                     /locus_tag="KPK72_000042"
     CDS             complement(38204..40189)
                     /locus_tag="KPK72_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG2487299.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            complement(40278..40817)
                     /locus_tag="KPK72_000043"
     CDS             complement(40278..40817)
                     /locus_tag="KPK72_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2487300.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQNIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIVMSLLLGI"
     gene            complement(41023..42336)
                     /locus_tag="KPK72_000044"
     CDS             complement(41023..42336)
                     /locus_tag="KPK72_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2487301.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGVFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            complement(42358..43083)
                     /locus_tag="KPK72_000045"
     CDS             complement(42358..43083)
                     /locus_tag="KPK72_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2487302.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            complement(43118..43363)
                     /locus_tag="KPK72_000046"
     CDS             complement(43118..43363)
                     /locus_tag="KPK72_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2487303.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            complement(43714..45714)
                     /locus_tag="KPK72_000047"
     CDS             complement(43714..45714)
                     /locus_tag="KPK72_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2487304.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(45745..47463)
                     /locus_tag="KPK72_000048"
     CDS             complement(45745..47463)
                     /locus_tag="KPK72_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2487305.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(47689..48927)
                     /gene="fabF"
                     /locus_tag="KPK72_000049"
     CDS             complement(47689..48927)
                     /gene="fabF"
                     /locus_tag="KPK72_000049"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG2487306.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(48956..49180)
                     /gene="acpP"
                     /locus_tag="KPK72_000050"
     CDS             complement(48956..49180)
                     /gene="acpP"
                     /locus_tag="KPK72_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2487307.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(49234..49983)
                     /gene="fabG"
                     /locus_tag="KPK72_000051"
     CDS             complement(49234..49983)
                     /gene="fabG"
                     /locus_tag="KPK72_000051"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG2487308.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(49983..50933)
                     /gene="fabD"
                     /locus_tag="KPK72_000052"
     CDS             complement(49983..50933)
                     /gene="fabD"
                     /locus_tag="KPK72_000052"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG2487309.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(50949..51878)
                     /gene="fabK"
                     /locus_tag="KPK72_000053"
     CDS             complement(50949..51878)
                     /gene="fabK"
                     /locus_tag="KPK72_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG2487310.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(51987..52973)
                     /locus_tag="KPK72_000054"
     CDS             complement(51987..52973)
                     /locus_tag="KPK72_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG2487311.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(52999..54021)
                     /gene="plsX"
                     /locus_tag="KPK72_000055"
     CDS             complement(52999..54021)
                     /gene="plsX"
                     /locus_tag="KPK72_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG2487312.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(54032..54589)
                     /gene="fapR"
                     /locus_tag="KPK72_000056"
     CDS             complement(54032..54589)
                     /gene="fapR"
                     /locus_tag="KPK72_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG2487313.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(54796..54972)
                     /gene="rpmF"
                     /locus_tag="KPK72_000057"
     CDS             complement(54796..54972)
                     /gene="rpmF"
                     /locus_tag="KPK72_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG2487314.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(54997..55521)
                     /locus_tag="KPK72_000058"
     CDS             complement(54997..55521)
                     /locus_tag="KPK72_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG2487315.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(55614..56810)
                     /locus_tag="KPK72_000059"
     CDS             complement(55614..56810)
                     /locus_tag="KPK72_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG2487316.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            57273..58553
                     /locus_tag="KPK72_000060"
     CDS             57273..58553
                     /locus_tag="KPK72_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG2487317.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNLIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(58816..60207)
                     /locus_tag="KPK72_000061"
     CDS             complement(58816..60207)
                     /locus_tag="KPK72_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2487318.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDVYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(60218..61438)
                     /locus_tag="KPK72_000062"
     CDS             complement(60218..61438)
                     /locus_tag="KPK72_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG2487319.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(61452..62240)
                     /locus_tag="KPK72_000063"
     CDS             complement(61452..62240)
                     /locus_tag="KPK72_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2487320.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(62257..63531)
                     /gene="larA"
                     /locus_tag="KPK72_000064"
     CDS             complement(62257..63531)
                     /gene="larA"
                     /locus_tag="KPK72_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG2487321.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(63649..64362)
                     /locus_tag="KPK72_000065"
     CDS             complement(63649..64362)
                     /locus_tag="KPK72_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2487322.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            64487..65746
                     /locus_tag="KPK72_000066"
     CDS             64487..65746
                     /locus_tag="KPK72_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG2487323.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            66038..66604
                     /locus_tag="KPK72_000067"
     CDS             66038..66604
                     /locus_tag="KPK72_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2487324.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(66805..68010)
                     /locus_tag="KPK72_000068"
     CDS             complement(66805..68010)
                     /locus_tag="KPK72_000068"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2487325.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(68113..68219)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(68311..68679)
                     /locus_tag="KPK72_000069"
     CDS             complement(68311..68679)
                     /locus_tag="KPK72_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG2487326.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(68731..70008)
                     /gene="obgE"
                     /locus_tag="KPK72_000070"
     CDS             complement(68731..70008)
                     /gene="obgE"
                     /locus_tag="KPK72_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG2487327.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAIRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(70119..70409)
                     /gene="rpmA"
                     /locus_tag="KPK72_000071"
     CDS             complement(70119..70409)
                     /gene="rpmA"
                     /locus_tag="KPK72_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG2487328.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(70423..70740)
                     /locus_tag="KPK72_000072"
     CDS             complement(70423..70740)
                     /locus_tag="KPK72_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG2487329.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(70755..71066)
                     /gene="rplU"
                     /locus_tag="KPK72_000073"
     CDS             complement(70755..71066)
                     /gene="rplU"
                     /locus_tag="KPK72_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG2487330.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(71167..72588)
                     /locus_tag="KPK72_000074"
     CDS             complement(71167..72588)
                     /locus_tag="KPK72_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG2487331.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(72606..73307)
                     /locus_tag="KPK72_000075"
     CDS             complement(72606..73307)
                     /locus_tag="KPK72_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG2487332.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(73309..75153)
                     /locus_tag="KPK72_000076"
     CDS             complement(73309..75153)
                     /locus_tag="KPK72_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG2487333.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            75472..76665
                     /locus_tag="KPK72_000077"
     CDS             75472..76665
                     /locus_tag="KPK72_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG2487334.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(76877..77959)
                     /locus_tag="KPK72_000078"
     CDS             complement(76877..77959)
                     /locus_tag="KPK72_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2487335.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(78523..79314)
                     /locus_tag="KPK72_000079"
     CDS             complement(78523..79314)
                     /locus_tag="KPK72_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487336.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(79318..79647)
                     /locus_tag="KPK72_000080"
     CDS             complement(79318..79647)
                     /locus_tag="KPK72_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487337.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(80132..80545)
                     /locus_tag="KPK72_000081"
     CDS             complement(80132..80545)
                     /locus_tag="KPK72_000081"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG2487338.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(80586..81716)
                     /gene="rodA"
                     /locus_tag="KPK72_000082"
     CDS             complement(80586..81716)
                     /gene="rodA"
                     /locus_tag="KPK72_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG2487339.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(81801..82085)
                     /gene="minE"
                     /locus_tag="KPK72_000083"
     CDS             complement(81801..82085)
                     /gene="minE"
                     /locus_tag="KPK72_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG2487340.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(82103..82900)
                     /gene="minD"
                     /locus_tag="KPK72_000084"
     CDS             complement(82103..82900)
                     /gene="minD"
                     /locus_tag="KPK72_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG2487341.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(82924..83607)
                     /locus_tag="KPK72_000085"
     CDS             complement(82924..83607)
                     /locus_tag="KPK72_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG2487342.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(83705..86683)
                     /locus_tag="KPK72_000086"
     CDS             complement(83705..86683)
                     /locus_tag="KPK72_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG2487343.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRMLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(86695..87183)
                     /gene="mreD"
                     /locus_tag="KPK72_000087"
     CDS             complement(86695..87183)
                     /gene="mreD"
                     /locus_tag="KPK72_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG2487344.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(87198..88091)
                     /gene="mreC"
                     /locus_tag="KPK72_000088"
     CDS             complement(87198..88091)
                     /gene="mreC"
                     /locus_tag="KPK72_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG2487345.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNTKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(88101..89111)
                     /locus_tag="KPK72_000089"
     CDS             complement(88101..89111)
                     /locus_tag="KPK72_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2487346.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(89232..89891)
                     /gene="radC"
                     /locus_tag="KPK72_000090"
     CDS             complement(89232..89891)
                     /gene="radC"
                     /locus_tag="KPK72_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG2487347.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(89936..90520)
                     /gene="maf"
                     /locus_tag="KPK72_000091"
     CDS             complement(89936..90520)
                     /gene="maf"
                     /locus_tag="KPK72_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG2487348.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARNMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(90740..91711)
                     /locus_tag="KPK72_000092"
     CDS             complement(90740..91711)
                     /locus_tag="KPK72_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG2487349.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(91727..92302)
                     /gene="rsxA"
                     /locus_tag="KPK72_000093"
     CDS             complement(91727..92302)
                     /gene="rsxA"
                     /locus_tag="KPK72_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG2487350.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(92317..92910)
                     /locus_tag="KPK72_000094"
     CDS             complement(92317..92910)
                     /locus_tag="KPK72_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG2487351.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(92923..93492)
                     /locus_tag="KPK72_000095"
     CDS             complement(92923..93492)
                     /locus_tag="KPK72_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG2487352.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(93492..94469)
                     /locus_tag="KPK72_000096"
     CDS             complement(93492..94469)
                     /locus_tag="KPK72_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG2487353.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(94492..95829)
                     /gene="rsxC"
                     /locus_tag="KPK72_000097"
     CDS             complement(94492..95829)
                     /gene="rsxC"
                     /locus_tag="KPK72_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG2487354.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(96045..96989)
                     /locus_tag="KPK72_000098"
     CDS             complement(96045..96989)
                     /locus_tag="KPK72_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487355.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(97100..98374)
                     /locus_tag="KPK72_000099"
     CDS             complement(97100..98374)
                     /locus_tag="KPK72_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2487356.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSKESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(98506..98877)
                     /locus_tag="KPK72_000100"
     CDS             complement(98506..98877)
                     /locus_tag="KPK72_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2487357.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
BASE COUNT        30968 a        18347 c        10638 g        39179 t
ORIGIN      
        1 gaaaagaaag aggtatgact caaaatgaat tagcagaaaa agcgaattat agtagagcat
       61 atatttctga tttggaaaat aagagataca aaaatattaa tatttctaca atcatagatt
      121 tatcacttgc tttagaaata gattttttag aattgtgtcg atattattgt gaagaagaat
      181 taagaagaag aaaattaatg taaaatatac ttttaaataa aaaatagagt agttcaaact
      241 actctattct tgtccacata gttaatataa aactcaaaat agtcagcgat attttttcaa
      301 gtatcttggt catttacata ccatatagtt aatataaaac ctcaaaataa actaagtatt
      361 ttcaatatct acacatatac aactcgctca aatttgcagt gaaccatgag tagtgcaatt
      421 gataagattt atcacatacc ctcaatgcct catatctcaa ctgttaagcc ctattttatc
      481 aaaaatatcg ctcactgcaa aatctctaca tttttattat atcaaaaaaa aatattattt
      541 ttgaatatct gtactaattt gtgatataat aaaagcaaga agaactacaa tctattttgc
      601 gttagagtgg agttcatcat aaacagagtt tattttttgg atttaaatat aaatttaaat
      661 tcaactcgta agtcactctt tgtgcgagag tggctttttg cttttttgaa tagtttactg
      721 attaagtaaa ctaccacact agctgttaaa ctagctaaaa cattaaacaa aaagttatcc
      781 atgaactcac ctcccttcaa atcgttggga ggataatctt ttgtacacga actccactct
      841 atagattgta agttaaatct tcttgctaca attattatat catattatgg taaattatat
      901 aattaaaaac acttgtttaa attctattat aattaaaata agtacatata aaaatgtact
      961 tatttttata aaaatattat taaaactcta aattataata tataatagta cctaaggagt
     1021 gtgattactt attatgagtg ttgccatata cttaagaaaa tcccgtgctg atgaagaggc
     1081 agaaaaacaa ggcgaatttg aaactttaag tagacataaa tcaaccctac taaaactagc
     1141 aaaagaacaa aacttagatg taattgagat aaaagaagaa ttagtttctg gtgaaagtat
     1201 aatacataga cccaaaatgc ttgaacttct aaaagaagtt gaagaaaata aatatgatgc
     1261 agtcttagta atggacttag acagacttgg tcgtggagac atgaaagacc aaggtatcat
     1321 attagaaaca ttcaaagaaa gtaagaccaa aattatcaca cctagaaaaa catacgacct
     1381 aacagatgaa tttgatgagg agtattcaga atttgaagca tttatggcaa gaaaagaatt
     1441 aaaacttata tcaagaagga tgcaaagagg tagaataaaa tcagttgaag aagggaattt
     1501 cataggaacc tctgctccat ttggatatga tgctgtaaca actggtagaa aagaaagaat
     1561 acttgtacca aataaagatg ctgatgtagt taggactatc tttgacttat atattaatga
     1621 agatatgggt tgtagtaaaa tatcaaaata cctaaataac ttaggcatta aaactgctac
     1681 aggtgctaat tggtacaatt ctgcaattac aaatataata aaaaataagg tatattgtgg
     1741 ctacattcag tggcaaaaaa aggactataa aaaatctaaa aatccaaata aaataaaaac
     1801 tgttaagcta agacctaaag atgaatggat tgaagcaaaa ggaaagcatg aacctttaat
     1861 aagtgaaata acatggaaaa aagctcaaaa tatactcaaa aaaaatggtc atgtcagcta
     1921 tggtaatcaa ataaaaaatc cacttgcagg aatagtgatt tgcaaaaatt gtggtagacc
     1981 attagtatat agaccatatg cagaccatga ctatataatt tgttatcatc ctggttgcaa
     2041 taaaagttct cgatttgaat ttattgaagc tgccatttta aaatctttgg aagacactgt
     2101 aaaaaaatat caattaaaag catctgatat agacttagat aaaaataata aagatagtaa
     2161 tatagaattt caaaaacgag tcttaaaggg cttagaaact gagttaaaag aattaggcaa
     2221 gcaaaaaaat aagttatatg atttactaga acgaggaata tatgatgaag atacatttat
     2281 tgaaaggtcc aacaatataa gttcaagaac tgaagaaatt aaagattcaa ttaagacagt
     2341 aaaaaataga ttgagtactg ttaaaaaaga caattctaaa ataatagaag atattaaaac
     2401 tgtgttatca ctttaccatg attcagattc ccttggaaaa aataaactcc taaaatctgt
     2461 aattaataaa gcagtttatt ataagtctaa agaacaaaaa ctagattctt ttgaattaat
     2521 ggtccatttg aaattacatg aagaccagta ataccaataa catataaaga gtagtgtgta
     2581 atgaaataag tcttttagat atacttggta cagaggctaa ctatgttctt gatgaagttg
     2641 aactaaaagt tcaagttgga aaactttacg aacaactaaa caaaattctt actcctagag
     2701 aaagagaaat tgtccagtta agatatgggt taactcctta tggctataag acacaaagag
     2761 aaattgcaca aaaactcgat atatcaaggt catatgtatc tagaatcgaa aaaaaagctc
     2821 ttaaaaaact agaaaaagag cttgttcaag aaagttaata ctatatttta aaaggatata
     2881 tcgtacatca ttagacaaaa ttttctatct aaaatcaaag ttacgatata tccttaaatt
     2941 ttatagtaca taatttattt tatactttca catatttcct taaatatagg tgttgcagat
     3001 ttatttcctt tttgagttcc ttctactaac acagttatta catacttagg tctttcttca
     3061 ggataaaatc ctgttatcca tccgtggtct atagcttttt tatttaaact gctttgagct
     3121 gtaccagttt taactccaca accaccttca atatctttta ggtctttagc tgtaccaatc
     3181 ctagatacag acttcatata ttctttaact tgtgtacaca catagggaga aataagttct
     3241 tctttttttg aagacttata agtttttatt gtattcatat tattgtccac aagactttta
     3301 tataagtata gtggtttgaa tgttccatta ttagctatta tttgagtcat ttgatttatt
     3361 tgaagtggtg taaattctat attttcttga cctatagcta aatttcttat tgatatgttt
     3421 tttggagctt ctctaatttt ttcttcatca agaccaatat caaccttttc aaataaatgt
     3481 aatttttcag cagatttgag tatcttttct tttccaagtt tcatagctat atctaaaaat
     3541 gcaggattgc atgaattaga aaaagcttgt cttaagtttt ggaaaccatg accatctctt
     3601 ttattacatc gcagtatttc attagtattc ccaactttag tcttacctgt acagttgtaa
     3661 gtatagttct catctataac accattttca agtgcagaaa ataaaactac catcttaaat
     3721 acagaccctg gtggatatgt cgcttttata actctatttt caaattctcc attttttcct
     3781 tttaaacttt ttgatatgtc attttggtca aaattaggtc tagagcacat tgcaagaatt
     3841 tcacctgtag atgcttccga gattattgct gctgtaggat tttcttcttt atttagtatt
     3901 tgttctaatt ttttttgaat gttataatca atagtagttt ttagatgtct atctttatcc
     3961 ttattatcaa cagtttttac acttcccttt agtatgttta gagatttttc atttcctgca
     4021 tctccagctt taaaagcact tatatacttt tcatttgaat ttctaagtac actatccata
     4081 ctcttttcaa ttcctgattg acctgattta tcatcttctt ttatatatcc tattgtatgt
     4141 gataataaac catcttttgc gtacctcata gttttttctt ctaccattat tccatttttc
     4201 tcaagttctt ttttcatact cttatcgata tttttagtct gaatctgtat tatagggcgt
     4261 gtcaattgct cttgtacagc tttatatata tctaaatcat tcatcttagt agctttctta
     4321 ataagctcca aaattttata gttattattt aatttttctt tttcaacaat taatacctga
     4381 gatttacttg tatctgtaag cttcttgttg tttctatcat agattattcc tcttccacta
     4441 tttaactcga ctttctcaac gctttgtgat tcaacacttt gtttgtattt atcacctttt
     4501 aatacttgta tatcaactag cctataaatt aaaacactat aaattattaa aattatagta
     4561 aatatacacc agcttctctt tcccactttt ttcaaaaatg gagtcttctt ttttgacatg
     4621 aaatcacccc ttagataatt tcattattga caatttctaa ggggctaata ctatttttat
     4681 caatttttta ttattcttca accttttctt ctataacttt tctaagcata taatctttac
     4741 caacttttac tgatagtttt aacttcacta tttgtctagg atgaggagct gattctatag
     4801 cttctccttc ttcattgtac atttcttcaa ttttagtaaa catagtttct ttatatggtc
     4861 ccataacttc tatttcatca ccaacacaca ttttatttct ttgctcaaca acaataagac
     4921 catcttcatc ttctgtttct ctaactatac ctataaaatc atagtttcta acatatgatg
     4981 ctgattgata attttggtct tctgttgttg gtttattcaa gtaaaaacct gatgtaaaat
     5041 gtctatgact accttttttt agttcttcca tccacattgg attgaacttc cagttttctg
     5101 ggtctctgta aaattcatct attgccattc tgtatgctct tacaacagta gctacatagt
     5161 atgcagtctt cattctacct tctattttta aacttgttat accagatttg attaactctg
     5221 gtatatactc tatcatacat aaatcttttg agttaaagaa gaatgtaccc ctttcatctt
     5281 cgtatattgg aaaatactca ccaggtcttt tttcctccac caaatgatat tgccatctac
     5341 aagattgagc acaagaacct ttgtttgcat ctcttccagt catatagttg cttattaaac
     5401 atctgccaga ataagaaata cacatagcac catgcataaa cgcttctata tccatatcta
     5461 aaggtgtttt tgctcttatc tcagatattt catcaaaaga taattctctt gccactacaa
     5521 ctctttttat tccatgctcg taccagaagt ttgcacttag ataattggtt gtatttgctt
     5581 gtgtacttaa atgtatttcc atattcggaa ttactttttt aatgacacta agcactcctg
     5641 ggtcagctat tataactgca tctattccta tttcttctag ttccaacaaa tagtcttcta
     5701 attgtagaaa atcttcatta tgaggtatta tatttactgt tacgaatact ttcttgcctc
     5761 tttcatgtgc aaaagctact ccctctgcca tatcttcctt actaaaattc ttcgctgcag
     5821 acctcattcc aaaaatttct ccacctatat aaacggcatc tgctccatat gttatagcta
     5881 ttttcaatct ctccaaatca cctgctggag ctaaaagttc tactttattt aattccatct
     5941 acatcacctc ttttactttc tttataactt agtgctacac catcacctat tggtaccaat
     6001 gatgtgctta aatagtcaca attacatata taatctaaat aatttctcat tcttttaact
     6061 atagtctttt ttcttcttac aacaaaatca tcatgagcaa ccataccttt atacaaaata
     6121 ttgtcagata ctaaaagtcc cccatctttt aatttatcta taactaaatc aaaaaatagc
     6181 ttatattgac ctttggctgc atctataaat ataaggtcaa attcaccttg gacttgacta
     6241 agtttttctt ctgcatctcc ttctagtata gttatgttat tgtcaaatcc agctaatttt
     6301 atgttttctt ttgctttttc tatcattttt tcatttctct caacagttat tatatctgca
     6361 tcttttccta taatagttgc aaaaaaaata gaagaataac caattgcaca tcctacttct
     6421 aaaattctct ttggtttttg aacctttaac aagactttta ataaatctga aacttcttta
     6481 tgtattatag gaacactatt ttcctctgca taaatttcta aatcttttaa aaatccttct
     6541 ttttctttta atgtcactct tatataatct tcaaccaaat cattaactat attactcata
     6601 aaaacactcc ctaaaataat aaatattcaa gttttatatt aatttattat tttttaaatt
     6661 catattgtta tatatattta cactttcttt attataaatc taagaataac aattttcaat
     6721 aaaaaagccc aacattattc ttaccctgtt agacttttta taatcatggt ttattttttt
     6781 gtattttctt tatttgttgc ttttgtatct tttgattgtt tatcccttgc ttctttgtat
     6841 tctttaacgt attttaaatg gtcttgatag tttgtactat aattatttcc accatcaatc
     6901 ttagctacaa aatataaata gtctgttttc tctggatata atgctgcttc aatagatttt
     6961 ataccaggat ttgatattgg tgtaggaggt aatcccttat ttttataact attatatggt
     7021 gaatcaattt ttaaatcatc atatgttaca attttctttc tctcttcaaa tatatattgt
     7081 attgtagcat ctgattgaag aggcatacct accttaagtc tattataaaa cacacttgct
     7141 attataggtc tgtctttgtc aagaacagct tctttctcaa ttattgatgc catttccatt
     7201 acttcttgaa gagtcatatt taattctttt tgtttctttt taaacttgtc cgtgtattta
     7261 ctgtcaaata cttttaacat ttcgcttaaa atatcttctt ctgattgttt ctttgaatta
     7321 aaataataag tttctggata caagaaccct tctaaagagg ttattccatc ttccttcaaa
     7381 tatggaaatt tatcataaaa ttcttgaggt ttttttatta aattttcaaa agtagcttta
     7441 tctcctaaat ttttacttac taacattgct ataatttctt tagatgtagc tccctctgga
     7501 atagtcactt ttataccatc ctgatatatt ttacctgaaa ctaaaagact tattatatca
     7561 ttgtttgaat acgattgatt aagtaagtat gttcctgatt ttatagacgg agctttattg
     7621 ctaaccttta ccaaaagttt aaataatagt tcattcttta ttaatttatt ttcatataat
     7681 atgtcagata tttttccaac agatgctcca tttggaacgt cgataattac atcttttttg
     7741 ttatttttat cataaggacc tatttgtata aacacaaata tacctgctaa gataatcaaa
     7801 ataacaatta ttaatactgc tattttcaat ctattttctt taaagttcat aatataatct
     7861 actccttatg tatatttatc cattttctat tataaaacta gatactttaa ttttcaatca
     7921 tcttgtagaa taaacttatt tatattgtaa aaaatgtttt aaaatatata tttttggtat
     7981 aatttatagt ttatcaaaaa ataaaaaacc taacattatt tttgacatgt taggcctttt
     8041 ataatcactt ttctaaaatt ttctaaaact ttttaatctc ttctttagtt acaataccat
     8101 atattaattt atttttttct atttttgaat ctacaataga gtaggaatca ataaatcttc
     8161 cctttgccaa tgaaagcttt tgttcatcat tataatacat gtaagctaaa acttcacctt
     8221 catttacata atctcctact ttcttttcta aaattatccc tgctgataaa tctaaaatat
     8281 catctttagt ttctctacct gctccaagaa tcatagccga tacaccaaca tcttcagctt
     8341 taattttact tatataacct gatttagttg atttaactgg atatttatac tttgattttt
     8401 tgaataaaga ataatcatct actacatctt tattaccacc ttgattctct ataaactctt
     8461 tgagtttttg tatagcagta ccagatttta ttgattctct tattttttca acaccatctt
     8521 caaaatcatg tgctaccttt gataaaacca acatatatgc tcctaatact tcgcatagct
     8581 caacaaggtc tttaggtcca tttcctttta atgtctcaat tgcttctata acttctaaag
     8641 agtttcccac tgcaaaacct aaaggttcat ccatatctgt tacaatccct attgtatctc
     8701 tattcatacc agtacctata tcaaccatag atttagctaa ctcaaaagac tcttctaatg
     8761 tcttcataaa tgctccatca ccagttttta catctagcac aattgcatca gctcctgaag
     8821 ctaacttctt agacattaca cttgatgata tcaatgatat attatcaaca gttgctgtaa
     8881 catctctaag agcatatatt ttcttatctg caattgctac ctttgcactt tgaccacaaa
     8941 ctgctatttt agacttattt acagaatcaa taaatttact tatctccatt tctgtcgaaa
     9001 aacctggtat agactccaat ttatcaatag taccaccagt atgaccaaga cctctacctg
     9061 acatctttgc aactggtgct ccacatgaag caaccaaagg tataagagct atggtagtct
     9121 tatctccaac accaccagta ctatgtttat ctacctttat tccatctatc tcagataggt
     9181 ttatcatgtc tccagagttc atcattgctt ctgtaagatg tacagtttcc tgtttattca
     9241 tcttatttaa ataaatcgac attaatagtg cagaagcttg atagtcaggt atatctccct
     9301 ttgaatagtt ttctataaag tagtttatct cttttttact aagctctagg ttatctctct
     9361 ttttttttat aatatcatac attctcataa ttgaaatctc cattctaacc tataataatt
     9421 taaataaata ttaaacttta aaacactttc aaatgttgta gtattataaa aaaactgtcc
     9481 tttattagaa ataactttct atatcaagac agctttttat taattctttt caaattaaac
     9541 attaactatt ttattataaa cttcctcaaa aaatctataa taagctttaa cttataaatt
     9601 tttaaccgtt gttttaacta aattcataaa ttctgattta actttttgag tagtttctat
     9661 tacttcctca tgacttagtg gttgatttaa gatgccagca gccatatttg ttatacaaga
     9721 tataccaata actcctatat tagagtgaga agctactatg acttctggaa cagtagacat
     9781 acctacagca tcagctccca aaatttgtgc cattctcact tctgctggtg tttcatagtt
     9841 tggaccacta aaaaacatat aaacgccttc tttgacatct atattacatt ctttagcgca
     9901 gtttttaact acatctacat atttagaaga atatgcatct gacatatctg gaaatctagc
     9961 accaaatctg tcatcatttt ttcctactaa tggattactt ccactaaagt ttatatggtc
    10021 tcttataagc atcaaatctc ctgctttaaa ttctttattt acgcctccag cagcgttagt
    10081 tactataaga atctcgactc ctaattcttt cataaccctt actggaaaag tagcttcttt
    10141 ctgagaatat ccttcatagt aatgaaatct accttgcata gcaataactt cttttccttc
    10201 aagttttcca attacaagtt gtccagcatg tcctgctact gttgatacag ggaagtttgg
    10261 aatttcacta tatttaattg tgacagggtc ttctatatca tttgctaaat cacctagtcc
    10321 agaacctaat atcaaaccaa tcttaggttt tatattactt ttagaattta taaatttact
    10381 actttgtgct atcttttcaa acattccaat ctctccatcc tattaagcta agtttttatc
    10441 attttatagt ttaaaataaa tcttctttaa agcttgaccc atgtttaggc aactttacat
    10501 ttagtatatc tgcaacagtt gctccaatat cagcaaaagt atcttttact cctaaattaa
    10561 aacctttttt aatcttattg ccataaatca taacaggtat atattctctt gtatggtctg
    10621 tacctttata tgttgggtca tttccatggt ctgcattgat tattaaaata tcatcttctc
    10681 tcatattagc cattatttca ggaagtctac tatcaaactc ttcaagagct ttcttatacc
    10741 cttctgggtc tctcctatga ccatatttag aatcaaaatc aacgagattt gaatatatta
    10801 gacctttatt atcttgtttc atataattta aagtttcatc cactccatcc atattgcttt
    10861 tagtgtggat agcatctgta attccttttc cattaaaaat atcttctatt tttccaactg
    10921 ctaaaacatc aagtcctgat tcttttatat tatcaagaac tgttggttca aatgggtcta
    10981 gtgaataatc tcttctattt gaagtcctca caaactcacc ttttttctta cctatgaatg
    11041 gtctagcaat tactctagct actgcattat cccccatcat tatttcccta gcgattttgc
    11101 acatattata gagttcttct aggggaatta cttcttcatt agcagcaatc tgaaatacac
    11161 tatcagctga tgtatatact attacatctc cagtttttat ttgatgctct ccatactcat
    11221 ctattattac agttcctgaa gctggtttat ttcctactac ttttcttcca gtttttttct
    11281 caaactcttc tattatatcc ttagagaatc catgttcaaa tgtcttaaat ggtttgtcaa
    11341 ctattatacc agtcatttcc caatgacctg tagtagtatc ttttccctga gacatttcct
    11401 tacactttcc aaaacaacct attggttctt ttatactttc aaaaaaatct ataccatcta
    11461 tgtttcctat acctaaattt ctcatattag gaatctgaat atctggatat tccttaaata
    11521 tatttccaag ggtacttaca tccttactat caccaaattt ttcagcatca ggtaatgctc
    11581 ctatcccaac actatcaatt acaatccaaa ttactctgct catataaata tgctccttct
    11641 aaattataaa atttgctata tagtctattt agcttctgct ttttgtaatt ctacatcaac
    11701 atcatttcta ggatgtttct cctttatttc tcttctcacg tttttatcta tatgatttaa
    11761 atatacttgc aaactagata aattaacatt tcctaaaatt ttacttacaa cagcaatgtt
    11821 agcaccttca tttaacaaat gaatagcaaa tgaatgtcta agcattgtag gatttatatt
    11881 tttatctata ttggctagat tagaatattt ttttatgact ttccaaagac cttgtcgtgt
    11941 aaacctttga cctaatgaac ttacgaataa tgatttttca ccttctacag ccattttagg
    12001 tcttgcttct tttagatact tttctaagta aagttttgta atatcacaaa gtggtatcac
    12061 tctttgattt ttacaacatg aattacaata tatataatca atatctaggt caatatcctc
    12121 tatattcatc tctactaatt cagatacttt tataccagta ccatataata cttcaaaaat
    12181 agccttgtcc ctaatcaatt taggtgttgt taactttgga aaatttaata gtctttcaat
    12241 ttcttcttct gtaagtatat ttatattttc tttttttatt ttaggttttt ttatatcctt
    12301 tgcaggatta tttgtacata tatgatttaa aaataaataa tcatgaaaag attttataga
    12361 ggatatcatc ctcgctattg tagctattga tacattatct ttttccaact ttattaagta
    12421 acttataata tcattttcta ctatatctac tagtttaatg cttttttgat ttatatattc
    12481 catatatttt tttatatcca taaagtatga tgccactgta ttctctgata attttttttt
    12541 atcttttatg taatctatat atccctctat aatatccatt ataaactcct ttatttagct
    12601 aggaacttaa taataccaat acttgcagta tttaatatta attgcaaccc caatgatagt
    12661 gctattataa ttataatatt tagtatatac ctcttaatta aaaattgcat attttttcta
    12721 tttttctttt taaattcata aaccatttct ttaaaataac ttaaactaat taatgctaat
    12781 atgattgcac atggaataat aatcaaattt ttaaataata ttattaaaat catttttata
    12841 cttttaaatt ttaatgccaa tatacaacta tttattgtat aaccaattga catccctttt
    12901 aatacaaaag ctaccaacac tgctggaatt gttactacaa atagacttaa tagagctatt
    12961 gagcccataa agattgcatc tgattttaaa ttttgtatca ctgtatcttt tatgcttata
    13021 tttagattat aatactctac agcaggattt atattgttta atatattgtt actagaacct
    13081 ggcaatattt tatttatgta agacccaaca acaatagaca tcataaacaa caatcctata
    13141 ataattattt gtctatttaa ttctctaaaa tcattttttc tatatgttct tctcaattta
    13201 attccccctt gtcctttata aaataatatg ctttaggaca aagggttatt cattttataa
    13261 atattaattt ttaaacatat cttttactaa taataatcca atagatgttt ttgcatcttc
    13321 aacgtcattt tttaatacca tgttatatgc ttcttcaagc tcaatttcat agacatctaa
    13381 aaactcatct gcatctaaat tattctctcc tggagtaaga cctgttgcta aatatacaaa
    13441 cattatctca ttagaaaaac ctgctgaagt gaaaaattta tgtattagct taatattttt
    13501 tgcactatac cctgtttctt cttttaattc tctttcagca cattcttttg gactttcatt
    13561 tttttctagt ttaccagctg gaatttcaaa gattggcttt tctataggtt ttctaaattg
    13621 cttaactaac acaactttat tatcatctgt gattgcaact attcctactg ctccaccaac
    13681 ttctactaac tccctttttt gatatccttg ccctggaatt tcaacagtat caacctttaa
    13741 agtaattact ttacctgtat aaaccctatc actactaata gttttttctt ctaataccat
    13801 cttaaacctc cattaagtaa tatgctgctg tgcttgcagc tttgaaaaat gcctcatctt
    13861 gttcataatt tcttcccata ctctttactt ttagattaaa atagtctaaa tcctctttcg
    13921 ttttattatt atctatataa attatattat gctttttgtc taaaccattt aaattgattt
    13981 gctcttttat aaagttcaat ttttcatcat catatatagt tattggtatg tctacattta
    14041 catttacaat ttctttaaat acagtcatac tatgatgtga aataccttga tgtctttctc
    14101 gtttgtcagc aaaactaatt cttggaattg caattgctct tccttcaagt ttttttacag
    14161 catcaagaat agagccttgt tcaatccctg taaatccata ttttgttcct gtcccagcaa
    14221 ttccaggtcc catactgaca aatactgcat ccgcttttag tatttctttt gctgtaatta
    14281 atgcagtata tatatttata cattcataat ctccaccaaa cgcatttcct atagttattg
    14341 tactatctat taattttttt tcttttaaag tatccacatt tttacttaaa taaattggaa
    14401 gagatgctcc atcagtcatt atatacacta attttttatt tggattattt cttttaaatg
    14461 aagctgcaaa tggagttaac atactatgaa gagtccctac tacaacagaa agaccttcta
    14521 atcctttaaa attcgcaaat ttatcatgat aaatactttc ttgttcctca acagaatcca
    14581 cttttatttg aagaggcgta tatctaagtt tcattatatg tccaccttca gttaaagtag
    14641 attctatatt atttaaatta gttattacaa aatggtatcc acctgtacct aagctcagtt
    14701 ctactgctgt tgtatttaaa accacttcgt cacctatatt tatagttcca gatatttttg
    14761 gataattata tgccttttgt atttctccat ttatatttac tcttatatca tctagagttt
    14821 cagtttgact tactattgat tcaacaatcc ccactctttt gcttatcata atactacctt
    14881 cccaatttat ctataaaatt taatagttta tttttttcta ttatcttagt taatgagtat
    14941 ttctcagtga atatatgcat aataactaca aatattaacc atcctaatct aatgttaaat
    15001 ggatacccaa aagaaatgag cattccaata gatattccaa ggacgtttga accagtatct
    15061 cccatcatag cccttgcttt taaatcataa ttaaagtatg caagtacatt tggtactata
    15121 agtaatggaa gaacttgaac aaatcctgtc aatgttaaaa atacaattac cattatgaat
    15181 aaatatgctt ttattgccct tcctggtctt aaatccaaaa gattcattaa attagttgat
    15241 agagctatta ttagtgtatt tactattata tcaattatgt cttttgatat agcaacagaa
    15301 actataagcc ctacaaatcc accaaataaa gctttaaaac ctcctgttgt aagacttcct
    15361 ttaaataaac ttttaaaatg cccctttaaa ccacttacat ctctatttcc aatgacatca
    15421 tctagtatcc ctgcaaaaaa catggatatc ataccaaaca taaacataaa tatatacgct
    15481 aaattcttaa attctgtagt aaagaaagca agtattattc cattgattac aatcataggc
    15541 aaaaatacaa ttcccatact tactggtatc atatcttttt tatagtttgg tctaagtaca
    15601 tttccattta tcaataaatt tttaaatagt ggaattacag cataggttcc aaataatcca
    15661 gttaataata aaatggtata aagtatataa tctttcacta agatattacc tccattcttt
    15721 ctttttttgt cctaaaacct tttttatgtg ataatactgt ttccctctat gaataaaacc
    15781 ttttaaattt cttcctgttt cattatgagt catatttact aatacttctt taattttaaa
    15841 tccatacttt aatatatcaa tggtcattcc aacttctact ccatatccaa aaggtatttc
    15901 atcaaacttt tctaaaactt ctcttttaaa tattctttga cctgataaag ttgcatctaa
    15961 ctcaacacca gtcatctcaa acacagaatt tttagctaaa ccttttacaa atcccaaacc
    16021 tccttttctc tttgcaggag gaaacttggc tattgtaaca tccgcctcac catttaatat
    16081 aggtactatt aatttttcaa cttctttagc agaacttcct aaatctccat ccagaaaacc
    16141 tataatatct gcatttttca ttgctaactt gataccataa tttaaagcat atcctttacc
    16201 tctattttta tctaacttaa aaaatttaat cttttcattt tctagctcag atacaacctt
    16261 agcagtattg tcaattgaac catcatccac aactattatt tcatttattt gcttaatgtc
    16321 caatatgctt tctaatgtat attttattct actttcttca ttataagctg gtattattat
    16381 acttatataa gaatccattt tttctctcct taaaattatt tctatttaat agatggcaac
    16441 aatgaagttg cagtatccaa tctaccaaaa ttaccaacta tattaccttg ttgtagaaga
    16501 atcgctagag atatacttcc tataccttca tcaatattgt ctatagttgc aactttgttt
    16561 ttagaatata gttctacata agaagtttta actcctgtat tttgaacttc tactaaatat
    16621 ttattctctg atttaagttt tgaaactaca aatttttcta ttttttcaaa ctgttttgta
    16681 ctgtcttttg catcatttcc accagctatt acaacggaat catattttag atatgtatct
    16741 ccaataaaat taaactttat tacatctagt tcttgaagat atgttaaaat gtcacttgca
    16801 tcttcctttt taagagtgtc aattatataa ttaactgcat catttgcatt ttttacatct
    16861 gtacctagtt tagttgacat ttcctttaac ttttctggat ttgttatgtt ttcttttaat
    16921 attatattaa atgcaatgct tccattagat ttatttatta tgtcttctat attttttgta
    16981 taatcgtcat tttcattagc agatattatt cctatactct taccattcag cgaaccagct
    17041 aaaatcttat ctacattttt atttataaaa ttaactgcct cttcataatc tctatttaca
    17101 cttgctaagt cactgtcaag tttatcattt ttttctttta agatattaaa cttatcatcc
    17161 aaatcattta ttatgtttgc ttgttgttca cttaattgtt gattattatt taaattaaat
    17221 cctactaaca tgcctatacc caaagctata aatatagccc ctatagtgac tatataatat
    17281 ttcatattaa tatgcatatt tatcctccta aaatttacat ctgtaataat actctaaact
    17341 ttaattgcat tagttgtatg aactgttgaa ctcctggtga caatgatgca actaccaaca
    17401 caggaaataa tgctgttgct attaatgccc atatgtactt tatttttaat ttacttctgt
    17461 ataacaaatt aacaccttta gcatctataa gctttgaacc tatttttagt ctaactaaaa
    17521 aagtacttgc catacctttt cttccttttt caagaaaatc aatcatattt gaatgtgtac
    17581 caactgctac tattaactca gccttatact cataggctat aagcatagct atatcttcac
    17641 tagttcctgg tgcaggaaat acaactgcat ctaatcctaa ttcctcaacc ctcttaagac
    17701 ctggtgctct tccatctgta taagcatgta ctactatttc actagccttt tttaaagctt
    17761 catcacttac actatccata tcacctacaa tcacatctgg agtatatcca aactcaatta
    17821 aagcgtctgc tcctccatca acaccaacca atactggctt tacctcttct atataagaaa
    17881 gcatagtact taaatcttct ttgtagtcct gacctcttac aactatcaat acatgtttat
    17941 tagcataatt agttttcact tttggaattt ctacttctcc aagtataaat cctttttctt
    18001 ttttagcata atctatagta ttatctataa atctatccaa ttctacagct aaattctcat
    18061 aggcagcctt tatttggtgt gaaacctcat acttgtctaa aacttctcct gcacctaaaa
    18121 attttccttt tctatatatc tttccatcta caacttctat agtttctcct tcttttaatt
    18181 cttcaaataa ctcttctcca acattatcaa ttattagtat attattttca gttaatatcc
    18241 ctggaccttt attgggatac ctaccactta tagaaggagc cgcatttatg acaagtttta
    18301 ttttagcttc tactaaagag tttgcagcaa cttcatctat gtctatatga tttataactg
    18361 ctatttcccc actttcaact cttttagcaa gttttttggt ttttcgatct acttttatag
    18421 gggcctcgac tctcatattt aaacctccac tatcattatt aaaatcacat tatattatag
    18481 cattacttaa caataaaata aatgttttaa gtttgttttg catctttggc atttacaata
    18541 ctatatttca tcattttttt atttaacata ttccacaata cgcttaaaaa tacaaagttt
    18601 acctgcatca aagtcaatat ttatattaaa aaataaacca ctgtcagttt acaacttgta
    18661 aagacacata ctatatccat agtataataa aattattata ttgatatagt aagcaaataa
    18721 taaagaaaat ttttatattt attaaaatag cgaaacggta taaccctagt ggttataccg
    18781 tttcgctgct tgtaatacgc atttatagcc aatgccttaa ttaaaaagcc ttacttttta
    18841 tgtcttgttt atttaaccat actatgttct agtcttaatt tatcagcaat cattgctata
    18901 aattctgaat tagttggttt tcctttagta ttgtgtaccg tatatccaaa taattgattt
    18961 attgtgtcaa cttttcctct actccatgca acctctattg catgtcttat tgctctttca
    19021 actctacttg gtgttgtatt gaatttttta gctatacttg gatataactc ttttgttact
    19081 gcacctaaaa gctcaacatt atcaataacc atttttattg cttctcttaa atataaatat
    19141 ccttttatat gagctggtac tcctatttca tgtattatat ttgtgatttc tgtctctata
    19201 tttcctacat tcttaacaaa atcacttcta gtcatttgag tctcttgaac tggtctaggt
    19261 tttggctcaa cttgtgtaac tctattagaa actagctctc taattctgtt tataaacaca
    19321 acaaaatcaa atggctttac tatgtagtaa tctgctccta gatttattgc gctttgagtt
    19381 atcttatctt gacctactgc tgatagtact attatttttg gcattttagg tatatccata
    19441 gtattcaatt tttcaattac acctaatcca tctagatgtg gcattattac atctagtatt
    19501 aataagtccg gttgtgtctt ttttactaag tctaatgctt caattccatc cttagctatg
    19561 cctaatatat cgatatcatc ttcattagac aaatactctt ttaatacctg acaaaaatcc
    19621 ttattgtcat ctgctaaaac tattttgatt ttttccacta aaaatccccc cattaaaaaa
    19681 catcttctta ttacagaaaa ctaaactttt tacacaattg cttcctaatt atctactcta
    19741 aagcatattc gacactccaa aattatattc ctcctagata attgataaaa taataaaatt
    19801 ttttaatgcc tcacctacaa ttttatcatt tcatctataa aaataccata tccttttttc
    19861 ggattatctc taaaaacatg agttattgca ccaataattt tattattttg tattattgga
    19921 gctccactca tcccctgtac gatacctcct gtgtattcca ataacttgtc atctactact
    19981 tttattacca tatctctata atttccttta tcatatacta tactttctat ttttatatca
    20041 tagcttgtta tatttttgtt tttatcctca aaaagaatta ctgcatctcc tatttttaca
    20101 tcttgtggtc ttccaacttc tattaattgc ttttctttat ttgctacatt aaattcttta
    20161 ttatggtttt ctgtaatatt accacttata cctagctctg aattatttga gaaattaccc
    20221 attaaattgc tgtcattaaa atctcctttt attttaccta ctttttcatt actagctttt
    20281 actattttta attgttctgg cttatatata tatccttgct ttatctttaa tagttcatta
    20341 gtatccgaat cttttatggc atgaccgatt gctttgaact tttccatact agggtcgtaa
    20401 aaagtcatag ttccaattcc agatatttta tctcttaccc ataatccaag tttatacttg
    20461 ccattttctt ttttagtctc tattacttct gttttatact tattttttct ctcaaaagtc
    20521 acttcaactt tacttttttt aatcttattc aaaatctctg atacatcttg actatttttt
    20581 atccttttat tatctatttt tactatatta tcaccgattt gtatgcctcc aatatagtct
    20641 acatcctctt cctcatatcc taagaccaat acaccatctg tatttgcttt tactccaatt
    20701 atatttccca taggatatac aaatttttta tcatgattct taattgtaaa agcttctatt
    20761 tgagtctctt tattaacatt acaattttta ttaaaaatta taaccatggt gaaaatcgat
    20821 acagctccta atattatcca aatatttttg aaatttttat ttattttgtt catatatcca
    20881 tctactccta tgctgtatta ttatgtattt ataattcctt tttcctgaga atttattcat
    20941 ttaaaaagtt tatttaaaat cttaataagt gatttttaat acataatatg atagtattag
    21001 attgtaacaa aaagtttgaa gggcggtaat atcttgaaat taaatgtaag aattgcagac
    21061 ataaataatt ggcatgattt agttagtttg tctgtagata aaagtcagtt agattatata
    21121 gaaagcaatg cactttctat tgctgaatct aaatttataa cagcttgggt acctgttgga
    21181 atatatgatg aaaattcttt aataggattt gctatgtatg gtcgtttaga agatgataga
    21241 gtttggcttg atagatttat gattgattca aaatatcaag gtaaaggata tggaaaagca
    21301 tcattagatt ttcttgtaaa tcatcttaaa aatgaatata actgtgatga actatatctt
    21361 agtatattcg aagacaataa aatggctatt aagctttata aagattttgg atttgagttt
    21421 aatggagagc ttgactatgg tggtgaaaaa gtaatggtat taaaatctaa ttgatattaa
    21481 gaaaaatata catatatcaa ggaggtgaag tcattatata aacatgtgta tataatggcg
    21541 gataaaatgg ctcaaaatat aactttacaa tttgttgaag aaaaagattt agaaaacctt
    21601 gaaaatgtaa aacttctatt tactgaatat tcaaattctc taaatataga cttatgtttt
    21661 caagacttta ataatgaatt aaaaacactt ccaggaaaat ataaaaaacc ttcaggctca
    21721 ttgatattgg catttgttga tgaaaatttg gcaggttgtg ttgctttaaa aaaacttgaa
    21781 ggtaaaatat gtgaattaaa aagattatat gtaagaaatc aatttagagg attaaaaata
    21841 ggaaaaatac tattggaaga aatcattgaa gaagctaaaa aatttggata tacacacatg
    21901 agattagata ctcttccaag catgaaaagt gcacaaggat tatatgaaaa gtttggtttt
    21961 tatgatatag aaccttatac ttataatcca attgaagggg ctagatatat ggaattaaaa
    22021 ttataatttt ttaatagagt ataagtaaaa ctactacagt tttatttata ctctatttta
    22081 ttgaacaaat ctatttgaat atttatttaa atatgtattt atttttctaa atcttgtaat
    22141 cccatagtta tatcttcagt tatatccttt gtatattcta ctccattatt gtatgtaagt
    22201 ttagctttaa tataaattat gccatcaata tcccttttag gaaaacttac ttcataattc
    22261 acttgatttc tatttgaatt attagttttg ccatcatcta agccttcagc taatttggct
    22321 ttgtatatag tgtctttttt tccttcattt acaataatat ctactttttc taatctaata
    22381 ttatcataaa ctttattttt tgacttttca ctctcatgat tgaaaagatt actattttct
    22441 actatttcaa taaagtattc tctattatct cttatcccag aattaaatat gggttgttca
    22501 ggcatataat cagatttagt aaatttttgc acatcagaac tttcagatat agctccactt
    22561 gtaactactt tatagtcata ttcacttcct gtatttatct taatttcaca tgcatatgat
    22621 aattctccat gttttgtcat ctcagtttca ttccatttat cactatcttc tttatacata
    22681 aaaaatactt ttccattttt tccaagttta cttaattgaa catctacatc aataacacac
    22741 tcttttttct tgattttttt tacttttaag tcaatactct ttgttatata actttttgac
    22801 atttcttcat ttatagtatt actaatatcc gacttagtac tcataatctc ttgtgacaaa
    22861 taatctaatt tattactaca actttcaata tttttattaa tctcacttac tgctaccaca
    22921 atataccata ataaaataaa aattacaatt gtaaaaatta agtgcatagt atctttttta
    22981 ttcattttat gcctcccctc actactacct taataaataa tattatactt aagcatttag
    23041 aaatttcaaa gttacaaatt tatacaaagt tcattccatt ttcaaaccta cttttcttca
    23101 atataggcac tagatatttt aaaataattt cattataaaa aataggtgta aagcatttct
    23161 ataaaaattc tttacaccta ttaaaatgct tgtttttcac taacattttt tagccaattc
    23221 aatgatttca cttgcatgtt caatagtttt ttctgttata ttattacctg ctataagtct
    23281 ggcaatttca ttttttcgtt gagattcatt taacttacta acattggtaa acgtcctatt
    23341 atttgaagta tctttctcta tacaataatg agtgtctgca tttgcagcaa tttgaggtaa
    23401 atgagttata catattatct gttttttctt tgctatatca ctcaatttct caccaactat
    23461 ctgtgctgct atcccactta ttccagtatc tatctcgtca aacactaatg tatcaatatc
    23521 atctatatca gccaatatgg ttttaaacgc taacataaat ctggacatct caccacctga
    23581 tgcaacttta tatataggct taatatcttc tcctaagtta aaagatatca taaattcaat
    23641 atcatctact ccatctaaag taaatggacc tttttcaaaa ttaaccttga acattacatt
    23701 tttcatattt aagcttttta attcatcaag aagtattttt tctaaagtag ttgctacttc
    23761 tattcttgct ttagtcaatt tactggcttt tatttttaaa tcttcttcta tattcatcag
    23821 tttgcttctt aactgttcaa ctctttcatc tctgtttaat atttcatcta atctatcttt
    23881 aatttttcca taatactcaa atatatcatc tatactgtct ccatattttc ttcttaagtt
    23941 attaatctcg tcgattctaa gctcaatctg ctctagctca taaggttcaa agtctatatt
    24001 atctttgtaa tttcttatct caccagatat gtcttgtaac tcatacatga ttctttctac
    24061 agtatcacta tattcactta aaacactgtc atatttagat atgtcattaa gttccttaga
    24121 tgcaagacct attaggtcaa taacattata ctcaccatta tgtaacttgc tataactcga
    24181 atttagatta ttatatatct tttcactatt tctataaact tctctttgtt ttaataaatc
    24241 ttcatattca ttcttattta gatttgcagc ttcaatttca tttatttgaa atcttaataa
    24301 atctatttct cgttgaattt gcatctcatc tttattttct gtcaagcaat ttaaagcttt
    24361 ttttacttca ctatacttat ggtacacctt tttataagca attttaaact cttccaattc
    24421 attttctcca aaaaggtcta gaaattttaa atgagtatct ttattaaaca aagcttgatt
    24481 ttgatgttga ccatgtatat ctataagtgt agatgcaatt tctttaagaa gagacacttt
    24541 aacagtcctt ccatttactc tagcagtact cttaccatct gagtatatca gcctagttat
    24601 taccaataaa ttatcatctt ccatacttat atcattttct tctaatattt ttttaagata
    24661 tctattgtct gaaaaaaata cagcctcaac aagtccctta tctgttcctt ttcttaaaaa
    24721 agacctatca tatttatcac ctaaacaaag tccaagtgca tctattatta tagattttcc
    24781 tgaaccagtt tcacctgtaa gtatatttaa gtttttatca atattcaacc ttagttcttc
    24841 aactaaggca caatttttca tatataattc gaggatcaaa tctaagcccc cttttaacct
    24901 atttgtctaa attatgagaa gcttcaacaa cttctactat tttgttattg tatgtctcac
    24961 catcaaattc atatccttca tttccatttc tcaaatgaat tagatattct atattacctt
    25021 caggaccttt aataggagaa aaatctaacc ctaaaatctc aaatccattt tcgactgaga
    25081 aatcagaaat catttttata acttctatat gtgtagattt ttctctaact actccttttt
    25141 tacctacttt ttctcttcct gcttcaaatt gaggtttaat taaagcgaca acttccccat
    25201 catgacgtac taactccttt gcctttggaa gtactaactt taacgatata aaagacacat
    25261 caattgacgc aaaatcagca aactgctttg tatcttctat tgtcacattt cttatattgg
    25321 ttctttccat acacacaacc ctatcatctt gtctcaattt ccatgcaagt tgaccataac
    25381 caacatctat agaaaatact tttattgctc catttttgag catacaatct gtaaaaccac
    25441 ctgtggatgc ccctatatcc atacatacct ttccatcaat agtcaaatca aagtttttca
    25501 ttgctttttc aagttttaaa ccacctctac tgacatatgg tattggattt ccttttactt
    25561 ctatactaca atcttctttt acttctgtac cagccttatc acacctttga ttatcaacaa
    25621 aaaccaatcc agccattatt gcttttttgg ctctttctct actttcaaag tacccttgtt
    25681 ctactaataa taaatctatt ctttttttca tacaattacc tttctgttaa aagctacttt
    25741 aatgataact aaagtagctt gaagtaacac acttgatatt tattctaatt gataaattct
    25801 atattttata taaattttaa tttacaatat ctacattaga tttttactca ataactaatt
    25861 tccttatttt atcagcaata ctttttggcg ataatcctac aaaatcatat aattcatcta
    25921 tattgccatg ttttacaaat tcttctggta ttgctatatt aagtatattt acgttatatt
    25981 cattatctat aataaactta tttattctac ttccaaatcc tccagttaca atattatcct
    26041 ctatagtaac tacattttta tgatttttta ataatgcttt taacatacct tcatcaattg
    26101 gctttaagaa tctagcatta actattgtcg gatttattcc ttctcttgat aatatttctt
    26161 tagcctctaa agcatgtttt accatacttc caatacaaag tatgactgta tcttgaccgt
    26221 catctaatac ctcatattta cccagtacaa tctctccata ctctccctta tctaggtaat
    26281 aactacttcc tcttgggtat ctaattgcta gtggacaatc taattttaat gacaaatcca
    26341 tcataagctc catctctctt gtgtcttttg gagccattac tacaatattt ggaatagaat
    26401 ttaagtaact taaatcaaac ataccatgat gagtttctcc atcatttcca actaatccag
    26461 ctctatctat aagaaaagta actggctttt ttgttatgca tacatcatga ataacttggt
    26521 catatgccct ttgcaaaaat gatgagtaca cagcaaagta aggtttcata ccattttttg
    26581 caagacctgc tgcaaatcct gttgcatgtt gttcagctat accaacatca taatatcttt
    26641 ttggatatgc actttcaaac aggtttagcc ctgtaccaga tggcattgca gctgttattg
    26701 ctactatatt ttcattacta ttagccatat caacaagttt ttctccaaca ctaattgaca
    26761 tagacttaac ttttgcagat gtaacacctg tctttatatc aaactttgat actccatgat
    26821 atttatcagg ttgttcttct gcaaatctgt acccttttcc ttttttcgta attacatgta
    26881 aaagaacagg cccttttttg tgtttagctt ttctcaatat atctattagt tctttagtat
    26941 tatggccatc tataggtcca taatatctta tacctaatga atcaaaaaat gaacagtcct
    27001 gaggagaaaa gctatacatt agtttatctt taaatctatg tgcagtctta gataaaattt
    27061 cacctgtttg tgtaacattt aaaattttat caacttcatc tgtcattttc tcaactgttg
    27121 agtttcttat aatacttgac agatatttag acattcctcc aacattttta tctatggaca
    27181 tctcattatc attaaggatt actatcatat tggtatctat atatcctaat tgatttaaag
    27241 cttcaagtgc catacctcct gttatagaac catctcctat cacagatata acactatagt
    27301 tttctttttt tatatcccta gcacaggcaa tacctgtagc tatagaaata gatgtactgc
    27361 tatgtcctgt atcaaaaata tcatgaggac tttcattctc ctttggaaaa ccacttagtc
    27421 cattaaactg tctcaaagat acaaaacaat cttttcttcc agtaactatt ttatgaacat
    27481 aagattgatg ccctacatcc catacaatct tatctttagg gctatcaaat actttatgaa
    27541 gtgctaatgt taactcaacc actcctagat tagaagctaa gtgtcctcct gtctttgaaa
    27601 cagattttac taagaacttt cttatatcct ttgcaagtaa atccatttct tctatactca
    27661 tattttttat atcctttgga gaatttactt tatctaaata tttatacata ttatcctcac
    27721 ctttattctg taaattttca atttacatca atattaaagc tgttactatt ccaagtaaag
    27781 ctccaaacca aacttctagt ggtgtatgac ctattaattc ttttaatttc ttctgctcta
    27841 tatgctttcc atgctgtata tctgccacca tttgatttaa tatagtagct tgtttaccaa
    27901 cagcccgtct aacacctgct gcatcataca ttataattaa ggcaagcacg gtaattatag
    27961 caaaatcagt agaattaaac cctttttcaa tacctaccac tgtagctaaa cttgtaacaa
    28021 aagaactatg ggaactaggc atacctcctg aaataagtat tcttgataac tcaattcttt
    28081 tttccttacc agtaaatatt tttataaatt gtgctaagaa acaagcaatc agacttattc
    28141 ccaaagcccc attgttaaaa atctccgaaa aaaaatccat ttttccctcc tcgattttag
    28201 ctgtatattt tagtattctc tatctattat atattcagcc aatcccttta aaaaatctac
    28261 atcatcagat aatttttcta tacttttctt tgcttcatca atcaaattat gtgcaatttc
    28321 tttagattta tctaaaccta aaagagatgg gtatgttgac ttatgatttt ctatatcact
    28381 tccaaccttt ttacctaact tagcttcatc tcctactatg tctagtatat catctactat
    28441 ctggaatgat aatccgatat tttttgcata tttagttatt tcttccattt gttcagaatt
    28501 tgctccacct atagtagctc ctgctctcat acagcctacc atcattgcag cagttttatt
    28561 catatgtata taatctaatt tttctttttc aatttgttta ttttcgcttt ctacatctac
    28621 tacttgacca cctatcattc catatatacc agcacctttg gcaatttcat ttatagcttt
    28681 tagatacttt tctggatttt ctttattgat tgagcctgca agcattactt caaaagcata
    28741 gtttaacaaa gcatctcctg ctaaaatccc catagcttct ccatatactt tatgatttgt
    28801 tggtcttcct cttcttaaat catcattgtc aagagctggc aaatcatcat gtattagtga
    28861 atatgtatgt atcatttcaa ttgcaatagc aaaaggaata gcttcatctt cattacctcc
    28921 aactatttta caagcctcca atgtaagtat tggtcttaat cttttacctc cagcacttaa
    28981 gctatagttc atagcttcaa taacagtttt ttgatacccg tcttctttag gcatatattc
    29041 tttaagtact ttttctacaa aacttgcttt ttcttttaga cattgtttaa attccaattt
    29101 tattcctcct ctattttaaa gtcttcttct attcccaatt gattaaattt acttattttc
    29161 aatttagcat catctaaaag cttattacaa tgtttataaa ggcttatacc ttcttcatat
    29221 aggcttaaag attcatccaa gctagcattt ttagattcta attcacttaa aatactttct
    29281 aatcttttat aagcttcttc gtaggttaaa ttcatactat ccctctttat tttcaatttt
    29341 atctatcata cattctaaat ttccatcttt taatactata tctatactat cctttatttt
    29401 taatcctttt atactattta ttacttttcc atttttttga gttatactat aacctctatc
    29461 catagtagct aatggactta aattatggag taatgctcca atttttacta atttttcatc
    29521 ttctaatttt aaattatttt ctattccaaa aattatttta tcatatattt tgtctaattg
    29581 tataacttta tcttttattg tgtacgaatc taaatagtta tttatcttat taaatactgt
    29641 ttctaaccta tattggtcta gctctatttg atttgtaaga gacttactca tcctactttt
    29701 tatgttttca agtttatagt ttatatcatc caatgatgga gtagcaatct cagctgctgc
    29761 tgatggcgtt ggggctctca tatcagacac aaagtcacaa atagtaaaat ccgtttcatg
    29821 cccaactgct gatattatcg gaatttgaga attaaaaacc tctcttgcta ccatttcatc
    29881 attaaaagac caaagttctt ctattgaacc ccctcctcta ccaactataa gtgtatcaac
    29941 attcttcata tgattgaaaa atctaattgc ttcacagatt tcttcagctg atttatctcc
    30001 ttgaaccgtt actggataaa gctttatatt aactttcggg tatcttcttt ttattacatt
    30061 tattatatct cttataacag cacctgttgg agatgttata actccaattg agtttggcat
    30121 tttaggtata ggtatcttgt atttagggtc aaataatcct tctttattaa gtttttcctt
    30181 tagtctatta aactctatgt gcaaatttcc tattccctct atttcaattt cattaatgta
    30241 taattgatac gcaccatctc tttcatagac tgatatatat ccattagcta taatttttac
    30301 accattatct aattttaaat tgcgattaaa attactttta aaaattacac agtttaactt
    30361 tgatgtttca tctttaagtg ataaatatac attcccacta ctatgaacct taaaattaga
    30421 aatttctcct ttaactttaa gattagaaag tattgggtcg tttattaaaa ttcttttaat
    30481 atatgagttt gcttcactta tatctaaagc tcttagtttc atactctttc tcctaaaaag
    30541 ttattcaatc ctattatagc acacccgaca gcattatcag ttgaatattg tggttctgta
    30601 aaataagctt ctatatgctt ttttcttaac ttcatgctta attctctaag aatatactta
    30661 cttgcagaaa ctccaccagc aaatactact tcatttacat tataagtttc acataaatac
    30721 actagagatt tagacatatt tctaactact gaatctaata ctaatttaga tatatattct
    30781 ttttgattag tattatctcc attatcattt ataatcttat ttacctgatt ttctaatcct
    30841 gataaattca tatacccatc tcttacagaa gttttcagac cactttctat tttaagatta
    30901 caatttaaag cattttcatc caaatacttt cctgaaggaa atttatatcc aagcttaatt
    30961 cctactctat ctataagttg accaaagcta atatctttac ttcctccaat tatatctata
    31021 ttatatccaa aattattgta taacttactt ttatcatctt ttttaatact tattttggca
    31081 attttaccta aattagtatc acatacattg tgatgactat cttgtttgct tgttaaaagt
    31141 atctctgtag taccaccaga catatgaaca gaaataaatt tattattatt ttttaattta
    31201 ctatttaata aacttgcttc aatatgattc tcttgatgag tagtctcata aaacctacaa
    31261 ccatatatac tagacaataa ttttccaaaa ttatgaccta cattgaatac aggcatatat
    31321 gagttttcaa caggtctcgg tttttttgat gagcaaactc cttctacatt aaacttatct
    31381 ttaaaagatt ttatcctatc actaagaatc cctaaattat tgatatgttg aaatacagct
    31441 tcactttgcc ttaaaccttt tgaattatcc cttacttcta acattatttt ttcattgaat
    31501 ataactttct tatctaaaga gatagctgct atagaagtag tatagcagct agtatcaatt
    31561 ccaattatta tattatttcc caatttcatc aacaacactt cccaatatac catttataaa
    31621 tttaggagac ttatcatcac aatatatttt tgcaagttct actgcttcat ttattgatac
    31681 tttattagga gtgtctaaat aaagtatttc acacacagac aatctaagta tagaaacatc
    31741 tacctttggc attctatcta ctgtccagtt tttagcatgc ttatttatta actcatctat
    31801 tttgtcatga ttttctttca atgctttgca tactgtattt atatattttt tgtctataac
    31861 atcttcaatt tcagtatctt ctaactttaa atttgtattt tttgagtatt gaagtgcaag
    31921 ttctttatat ctatttataa tatgttctga attatctttt aaaaaattat ctactttatc
    31981 ttctaaagtt tcaaaatctt ctttattaat atttatttgg tatataagtt tcattatata
    32041 ttctcttgtt gtactctttt gtgccctatc ttttttcatc aatcaaaatt ctcctttaag
    32101 tttaattatc catataaaat caattaccag caaaaaaccc tctgactttt cagagggcta
    32161 atattaattt ttcttagttg cttttgcttc ttctttatca actttatcct tcttaaagct
    32221 gattccttga atgtgtatat ttacttgaga aaccttcagt cctgtcattg tctcaacagt
    32281 attttttaca ttttcttgaa ctttaaaagc tgtatctggt attgacattc cataccttat
    32341 cgtaaccatt acatctaaat taacatcctc ttcttcgatt tgtattttta ctccattatt
    32401 ttttaacaac ttatctgtta atgttgctgt agtttctatg ccttcaactt ctaatgctgc
    32461 taaaccagct attgtagcta ttacatcatt agatattttt acttgtccaa atttattatc
    32521 ttccatatta aaacttacct cctgatttta aggaactatt tttataagta taataatacc
    32581 aaatacaagt ctactttgca agaaaataac ctcagttaat tctgaggttt atactttact
    32641 tattactatt attatttgta agtttaatat tttcatactt cacattggct tgttctgcaa
    32701 ctaaatcaaa tatttttgct gcatctttct tttctaattt agcttcattt acaactacat
    32761 taactttgtt atcgcttata taaactaacg cttcttcata tccttttgta ctcaaaaggt
    32821 tttctatttg aagttctttt tcttgaattt taactatatt taatttcata ttagaagctt
    32881 cttttctaga ttcttctgtt gtagatggat tatttatcat ttcatttaaa tcttgaacta
    32941 actcatttct ttgtttttct ctattcattt tcatatctaa aatataagat gcctttttca
    33001 tatttttctc tgatgataat tgagctttta tttccttact tgtctcagtt gccttttctt
    33061 ttactttact agctttgcta tcgactatat caatatcagc actttcctta ccaccttgtt
    33121 tatctacatt actagaatcc tctgtcttac tactatcact atcatcagta tttttttgca
    33181 actgtgcttc ttcatatgcc ttaaactctt ttgatgtttc taataaagat ttcttactta
    33241 gttgataatt cactgttcct actaccacta acattgcagt taaagttatt attacaaatc
    33301 ctcttccctt ataattaaac ttcattcatt tatccccccg gttatatgta tttatttaga
    33361 atatatttct acttgatgac cagctacttg taatgatgtt tgtactgcac tataaagtgt
    33421 ctctttaaca cttgggtcac ttgctccact tgctaccact atcactccct ttatctttgg
    33481 ctctgtagtt tttataacta cagggtcact tgaatttgaa gttatcattg ttttattttc
    33541 actagaagtc gtaactgttc tttctccacc ttgtgcatct ttttcctctg ttgtttctgt
    33601 tgtattattt gaattaaaag caggttgtat ttcttcactt gattcaaatg taaccattac
    33661 atcaacgtct cctgcaccat caattttaga tagtattgct gttaattttg actctaaatc
    33721 atctttttct tgttctttag tgacttgctt ttcagcattt ttattagtct ctgtttttcc
    33781 aacttgttta tcgcttttgc tactaggaaa acaagataat aatactaatg aaaccacaca
    33841 tattcctgct atagttatta gtgaatatac ctttctctta tctttgtcat ttaagttttt
    33901 caacattctc ccaactccta tttatcaatt tgtatatctt ctatagaaac atccagcacc
    33961 tttattaagt cgttttttaa tttttcttca tttaggttca acccatactc tgtacctttt
    34021 ttgaagactt gtttagaatt ttctttgtca ttagagttta tgttttcttc ctctctttta
    34081 gtactattat ttttattttc ttcattagaa ctgttattac tttttgcact attattcttt
    34141 tcttttattt ttatattatt tatatttgcc ccattaagct ctatatcttc taaatcataa
    34201 ccatattcat caagttttaa tttaagtact tcttttaacc catcctcata tcccttggat
    34261 agactattat ttccagtctc ttttgccaaa gcatttgtac tatctacata ctgcttgttg
    34321 tagtttccca tagattttaa tattctatct tctaaactca tatcttttga aaaaaatcct
    34381 actactggag ttattacaat aaatacaaac ataaagttta aaactaggtt gacataaggt
    34441 tttatcttag aacttggtaa aatcatatct actatattta ctataaatgc tcctatcaaa
    34501 acactaacta tccatgcctt tatgccctct aacatagcat cctcctctct aaaaacatta
    34561 gattaaatgt aattattttt cactgatata taaattaccc ttgacttacg acacttatcg
    34621 aggtcaagat agctacagtt acaaagaaca ttactgttat agcaattact gatgctagca
    34681 ttattatcat taaatttgcc acttcattta aaaaattaga tattccatcc tctccaacag
    34741 gctctacaat tattgctgct aatttgtaca ctactattac tgataaaatt tttattactg
    34801 gaagtaagca aattccaaca agtaagataa gccctattcc tccaaatata ttttttataa
    34861 gctgagatga agataataaa atatccacag agtcagatac aaatccacct acaacaggaa
    34921 taaaattacc tatagcaaat ttagcagtct ttacactgaa tttatcaaaa ctggttacat
    34981 acaatccttg tatggataca agacctaagt aaacagtaaa catagctcca atagaaacat
    35041 aatttatttg ttttacaaag gagaaaaatc tctttagcct tatatttttt gataaattgt
    35101 ttattattaa aattccaaat gcaacagtaa tagatacaaa tagaaaattt ttaaaaaata
    35161 catttataaa tgttactcct cctatgaaga taggattcaa agtagtcgac gttattggga
    35221 atccaattaa aagtaaaaat gttattaata ttggcattat aacttgcata agacctacag
    35281 tatggtctat tgcatcatag caaatctgta atacatcttt aaaaccaata agtgtaagtg
    35341 ataccattgt gataaatatt atatatgttg ctatttgact tactgcacct gatgaaaaag
    35401 agttttccag actctttaaa atagatgata acagagccag taccaaaatt actgctacta
    35461 cctttaaact tgctttaaat tcatcaaaca tcaatatctt caatccatct ttattgaata
    35521 aatctaagat atttttttct ccactaatta aatcttttac aaatgatttt aaattaacat
    35581 cattaataac tatttcttta tttatatagt cttgaatctc atttatatct agcttgtcta
    35641 attgaccatc tatatactta tctatgctat tttttgtttc accatattct ttatcttcac
    35701 tacttggtac ttcattagca aatatcataa tagcaaagat atttacaaaa aagaaagtaa
    35761 ataaaaaacc tataatcatt ggtaaaaatc tttttttcat aagcaaccat gcctctcttc
    35821 actctataaa cctcaataca acgaaagctc tctaatatac ttatattcat tccaagatac
    35881 acatctatgg aattatattc accaccattt ctacaatgga caaaagtata gggaaagaca
    35941 ttgacataac tattatttta cctccaaatt ctagttttga agcaatgttt ccttccccac
    36001 aatctttaca aagttgtatc gcaaattcca ttaagtatgc tatcccaatt aattttatta
    36061 ttaatgatat atacatagtc gggatgttag ccttgttagc caaatcttgt atactatcta
    36121 ctataaaatt tagcttaaac ataactgaca atagtattat tactccagtt attatagcta
    36181 tgaaatttgc tatctctggt ctatcttttt taattactaa acagagtgta gttgaaatga
    36241 ttgcaattcc tattaattgc attatttcca aattactccc tcctctttag taaagttgaa
    36301 acattgttct tacagtatta aacaagtcac ttatttctgt tattaccata cccaatacga
    36361 ttattacacc tgctaatgta gtaagacctg cccaatcttt tctatctgtt ttttccaata
    36421 tcatattcaa tactgatatt agaataccga cacctgcaac ctttaatatc aatgatattt
    36481 ccataataaa cctccccata ttaaatcaat ataatacata tacctatacc tatgaatgtc
    36541 actaattttt tatacaatac gccttttttg tttatatctt cctttgtctc ataggtttgt
    36601 tttttcaaat tttctataga taaatctatc attctttgtt gtgactctat atcgcttttc
    36661 cctaaagtaa gtattagatt tttcagttcc tcaatttctt tattgcctag ataagtttct
    36721 ttacttaaga catcaatatt ttctgatatt attatttcca aagttttact ttgttcattg
    36781 tgaagtcctt ctgatatttg atagaaaaat ttccaaaaac aaaactcttt atttccacct
    36841 atcctattaa agatttcttc taatgtataa agtccaaaag ataaatccat cctcaaaatt
    36901 tctaaaactc ttattaaatc gtttaattgt ttatgtctac gagtataagt tttgtatatt
    36961 tgttcaccta tcaaataact acttcctatc aagaaagcta taataattat tttaatttgc
    37021 aaaataccat ctcttttctt ctaaatcata aattttttca attgttcctg ctcctctttt
    37081 tgcagaaaga attataacct ttttaaataa ctctttatcc agaagtctat ttaattcttt
    37141 tctattttgt atatcctcta ttgaatctcc atgcacagta gttattaatc ctatacctcc
    37201 atttaatgct gtgtataatg cctttatttc tttttcactt ccaatttcat ctgtcacaat
    37261 aacatttggt gacatagacc ttaaaagcat tgtaataccc aagtcttttg ggcaagtttc
    37321 tattatatca gtccttattc ccacatccat ttgtggaaca cctagataag ctcctgctat
    37381 ctcattacgc tcatctacta aagccacttt taaaccttta aatccataat cttcattacc
    37441 attacttaaa tttcttacta tatctcttat taaagttgtt tttccacatt gaggtggtga
    37501 tattatcaaa gtattattaa tctgattttt tccctttatg atatgactta aaatcttatc
    37561 cgagcatcca ataatttccc ttgataccct tatatttaaa gatgatatat gctttatatt
    37621 tttgacttgt ccatcttcta ctattgcttt tccaactaac cctactctat gacctcctct
    37681 caaagttatg aatccttttt ttatgtcatc cataaatgaa tgtattgaat atttacatat
    37741 tatttgaaaa gtttgctcta catcttctct tgttactaca tatgagtttt gttgatttaa
    37801 atcaagtgtc atagtttttt gattgtaaaa ataatcttta gagtttgcat ttagtatcaa
    37861 tggtttttgt gagcgtaacc taatttcttc tatgttcaga ttattgtttg agactttttc
    37921 taccttttct cttatagttg tagaaagaga gtttattatt tcatcagaaa gtttattcat
    37981 ctcttgtcct cctcctttgt ttatataatt ctatttataa aggacaagga atataaccaa
    38041 aacatctata tttttttaat aatgtttata aataaaaaaa gtgaacccat ttatcttgga
    38101 ttcacttagt aagtataata taaactacta ttattaatct ttataatata tttaataagt
    38161 attaacaaaa agacacttca atcatgaagt gcctctataa catctacatt tcttttatta
    38221 gccctttctt ataagccatt aaaagtagtt ttaaatcttc tatttcctct tgtatcttta
    38281 ctccttcatc agtatctctc accatttttc tctttgcctt atgctctact acaacagttg
    38341 tcgataagat atcactttca tttgcaatgg cttcttcagc agagttaaat ggttcatgcg
    38401 atactaattg caaagttcta gaattgtata tcaaagtgta tccagctata ccagtagttt
    38461 tttggtaagc cctagaaaaa ccaccatcta taacgagaat tttaccatta gcttttattg
    38521 ggctctctcc atttttactc tccacaggaa catgaccatt tattatatga gattcatcaa
    38581 gttctaaatc aaattcttca aataatcttt tacaagccat ctcattttct ctaagtttaa
    38641 aatatgggtt cttattttct ttatgagtct ctttttcagc tataaagtat ctttcaaatg
    38701 tagtcatatc atctttacca aataatgatg aacatttccc agtccataaa taccacataa
    38761 tatccatgcc atacagtttt tcttctgcct tatctttaaa gaaaaatcct tctcttgcta
    38821 gagactccat cttatccata agagccttac ctttatactc ttttcccatt atattcatag
    38881 acataaagct tccatcttca tttaacggta cacatccatg aatcagtaaa tttgagttag
    38941 cctttaaata tatacttcct ttagaaaaca aaaatgaaac atgtttttgt aatttttcac
    39001 tacctctaaa tgaagatact aatttatcaa taacatttct ttcttccata gttaatttat
    39061 atgggtcttt tttgtctatt gtaggtaaat aagtatcttt taatttatat gttttacctt
    39121 ttaaattaat cgtaccttca tcatagttta tcatattaag caagagtcta tgttccattt
    39181 caaattcagg tcttcgtctt ataacctcac cttcaagctt aaattgaatt atacttattg
    39241 ctttgtgcat ttttgccatt aaagactttt ctgttgttgt tacactttgg tcattaacct
    39301 ttggaataaa ttctttacaa gggtcatctt tatacatttc tatagcaaaa gtggctaaag
    39361 gcaacagatt tattccatat atgtcttcta caatgtctaa attcgcatat ctagctgata
    39421 tcctcagtgc atttgctata caagtttttt cacctgaggc tgcacccatc cacaatatat
    39481 catgatttcc ccattgaata tctacacaat gatgttcaat aagcttatcg acaattatat
    39541 ctggtcttgg acctctatca taaatatctc ctattacatg aagtctatct acaaccaatt
    39601 tttgtataac tgtagaaata gcaattataa actccttagc tattcctatg tcaactattg
    39661 tttcaactat acttttataa tactcttctt tatgttcact tttaacatgc tcatgcaaaa
    39721 gttcttctat tatgtattta aaattttctg gtaacagttt tctaacttta gacctagtat
    39781 atttgctaga agcatattta caaagttcta taagtctata aatatttatt ctataaaaat
    39841 catctatatt ttcctcttgc ttaattatta ggtctaactt ttgttcagga taatatacaa
    39901 gagttgctaa catttttttt tcgctgtctc ttattgtatt tgaaaacaat tcttctattt
    39961 ttctctttat tacaccagaa ccatttttta atacatgtac aaatggttca tattcaccat
    40021 gtacatctgt tataaaatgc tctgttccct ttggaagatt taatatagcc tctaaattta
    40081 ttatttctgt acttgctttt gatatacttg gatattgttt tgataataac tttaaatact
    40141 taagcttgct gtttaaataa tcatctgata ttttacacag atttttcata attcgtcctc
    40201 caaaattata ttaagttata ttctttttta attataccat acttaatata cttttttcga
    40261 gataaaatgt acactaatta tattcccaat aaaagtgaca ttactattaa atcagtataa
    40321 acaccattat aataacattc tttctttagt atgctttcta cttcaaatcc aacttttttg
    40381 tataatccta tagcattata attttcttca ttagtgacaa gagttatctt cttggttatt
    40441 ccatttgatt tacaccattc tataaggtct aacataagcc ttttacccaa tccaattccc
    40501 caatattttt cttttacaac aattcctaat actccaacat gctttccttt tgctttttga
    40561 ttagaatcaa tcgttgcaat cccaactatt tcaccatcta taaatgcaat cagtttaaca
    40621 gaattatcag atgcttttgc tgattctata gcattttctt gttcctccaa agatatttta
    40681 tattcatcct ttccaaatga cagaaaatga gtttctccac ccacttcatt ataaaaatct
    40741 ataatgtttt gagcatcttc ccttactcct tctctaataa gtacgtctac accatttttt
    40801 aatgttattg ttggcataaa tttacctcct aataagtttt ttgtctataa acataatact
    40861 attgttcttc tattgttaca atatttttca cttattgata acaatttaag gctatattaa
    40921 aataaaaaat taataatatt ttaatatagc cttatagata cttatttgta tttaattttc
    40981 tgccaatgtc taattttaac tcaataaatt gttataacat aattaaacta ttttctttaa
    41041 attttcacca taaacttttc tattataaga cttcattata aagtataatg gtactccaat
    41101 tgccaaagct cctaatccca taattaattg atgcatagat gcttggaata acaaccatgt
    41161 tccaacacat acagcaatta gaggaatcac tgctccaaaa ggagttttaa aagtacctac
    41221 taattctggt cttttctttc taaacactaa tacggataaa gatgttggta tgtattgtat
    41281 gaatctagca actgcactta ttgctgctaa ttcagcaaaa cttccagata gagctattgg
    41341 tatagtgatt aacatagtag ctattatcgc tacatatggt gtaccttttt tatcttttct
    41401 tgcaaatatt ctcggtaaca ttttatcatc agctaaagct acacaacttc ttggatttac
    41461 aaaaacacca cacatacaaa ttccaccaac agaaaccaag gttccaaagg taaccaatcc
    41521 agctcccata tttccaaagc atattcttgc agcatctgcc actggtgtag atgtactaga
    41581 taatgaatct cctagtatac caacacatac aacttgattt aatatatata ttattgatgc
    41641 aattactatt acacttataa ttgctattgg tatatttttt tgtggattct ccatatcttc
    41701 tgctgcaact gctatagatt caaatcctgt aaatgaataa aacattaaaa gtgctgctgc
    41761 tccaaactct gttgctccag ctcctggagg taccattgtt gaagaagtaa aattaactcc
    41821 ttttataaaa aatataccta ttcctataaa cagtattaat ggaattagtt tacctacagt
    41881 tataacattt tgtacaattt ttgatagaga tacacctaat aagtttataa ttgtaagccc
    41941 aactatcatg gctacaatta taattttttg tatatgtggt tcacctgctg gagcccatac
    42001 tgctgataat gatgtaggaa aacctactat taaagctccc caagaaatac atcctattac
    42061 ccatttcata agacctactt caaaaccaca aaaatctcca aaagcttctt ttgtatatac
    42121 ataggcagca ccatttttat tgaatttacc tgcaacctca gcaaaacaga atgccataga
    42181 taaaactaac aacatatcaa atatatatac aaatatactt gctacaccaa atgatttcat
    42241 tgcatctcct ggtaatagaa aaactccagc tcctactact gcattaatac ctaaaagtat
    42301 aatacttatt aatcccattt tgttcttttc gttcatgttc aatcccccca aggtatatta
    42361 taaattcgaa caaatattta taaaatattt aaatagtttt tccattttat cacagtaatc
    42421 taccatcatt tctggatgcc actgtactcc tactacaaaa ttttcatcct ctttctcaat
    42481 agcttctatt ataccatctt ttgagtatgc tatggctttt aatccttttc caagctgatt
    42541 gacactttga tgatgataac tatttgtaag taattgtttt cctaaaattc cctctaaaat
    42601 acttccttct ttaacatcaa ctttatgagt agctacatgt cttttagaag attgtgtatg
    42661 ttttatataa cttccttcta taagattatt atcttgatat aaagttcctc ctaaacttac
    42721 atttaaaatt tgtagtcccc tacatacacc taatataggc tttttaagtt ctaatgctat
    42781 acttattgca attaaatcaa attcatccac ttctggatat ataaatgtcg tttcttctct
    42841 tgtttcttct ccatacagtt gaggattgat atcccatccc ccagatatta agactccatc
    42901 aaccatttca atctgttttt ttataacttc tttatctgta tttacaggaa ttattactgg
    42961 actaccgcca ccttttagca ctgcatttat ataatcatta tttacatatg acctttcaag
    43021 acctggaaac ataccatttt ccattattat taaatttcct aaaatcccta ttattggttt
    43081 cataattaaa ctcctaacta atcgattgtt ttatgtatta ctttatattc atataatttt
    43141 ccaatgctag agacagttgg tctgggcaag atgttggctt tgttttacac tctattcctt
    43201 ttaacttttg tattacatct tctatattca tgccttcaac taatactttt agccctttta
    43261 aatttccatc acatccacct ataaagttta catttgtaac tttattttct tttacttcaa
    43321 attcaatttc tgtagcacaa gtccctgttg ttttgtattt catcattaaa cctcccaatt
    43381 attttgtcgt cttatctcta taattagcaa tatgtatgcc aaaatctata attattttta
    43441 tttattattt atctagtatt ttcaatagtt ttattagttg tattttttac ttctaattct
    43501 atgtacactt ttcttacaaa taaaaatagg taatgggtta ttctaactta acctattacc
    43561 tatctaatcc tattttaaaa acctattaat atttaattat aaattacaat attctttctg
    43621 acataatata aattaatctc tataaatata aaagtttatt attatttaaa tatctaaatt
    43681 caaaatatgt gatttcaata tcaaatactt agcttaattt atattattat aaagttttat
    43741 cccctctgag gttatttgac ttcctcctct accagattta gatgatatgt atccacattc
    43801 acttaaataa gatagcacct ttctaacttt gctttcagaa atctcaatac cttcctgtat
    43861 aagcttttcg attataaacc ctcttccaat tccagaggat gactctcttc tttcatatag
    43921 aatcctcagt atttcctcaa attcttttct tgtataacca aatccatctt cataattgtt
    43981 agcacattct acccttatat atggtggtaa gtcatctcta aaaaccatac tatcacacat
    44041 tagacttaca taagaagcaa cattttgtaa ctctcttata tttcctggcc aattatattt
    44101 ttctaaaata gttataactt ctggcgaaag tcttatattg tttttcaaaa agaaattcat
    44161 aagtgttatt atatcttctt ttctattcct caatgatggt acatctattg gcaatacatt
    44221 tagtctataa tacaggtcct ctctaaattt tccttctcta atcatcttta gtaagttttt
    44281 gtttgttgct gctatgaccc taacatctat gtttataaca ttatgggaac ctataggcat
    44341 aacctgcttt tcttgtaata cccttagtaa ctttgtctgt agcaatggag gcatatctcc
    44401 aatttcatcc agaaatatag ttccattatt agcaagttca aatagaccct tttttccttc
    44461 tcttaaagct cctgtaaaag agcctttttc atatccaaac agttgacttt ctaatagact
    44521 atctggaact gctgcacaat taactgctat aaacggttga ttacttctat tggaagcatt
    44581 atgaattgat tgtgataata attctttacc agtaccactt tctcctatta ttaatacaga
    44641 aaggtctgat ttagatattt ttttagccaa ttctacacac tttaccatat ttttattttt
    44701 agtttttata tctttaaatg tatatcttgc tatctgacct tgttctctta atttattagt
    44761 aagattttgt tctaattttt ttatatacgt tatttcttgt aagttataat atatcccagc
    44821 tttattcccc atagaaaaaa tacttttttt atttacattt atatattttt tatttgtaaa
    44881 taccacttca tcaattattt ctttgtcttc aagtaaaata tttaaatctt cagaaatgat
    44941 atcttctatg tttttgcctt gtggattttc atttatacct aaaattttta aagcttcttt
    45001 attaaatgtg tttattatac catctgctga agtaaatatt attccatctt ttgatagatt
    45061 caatataaca tcttgttctt ctatttttaa aaacagttgc ctataattat ctttaactcc
    45121 attatctaaa cttattaaac tctcagaata tgccattaaa tttttacgta tttctttaac
    45181 atctattcta agaccattta ttatttctat aaaggtagat atatcaatat atctatttcc
    45241 aaggtcgatt actttattta tataacttgg tacttttccc tttactccag gtgttatagc
    45301 tatgtctata tttttataaa tttttccttc aatatatgga gtaaacttta aattttttac
    45361 tcctattttg taaaataagc ttatcgtttc taaggtagtt tcttttgtat catttacaac
    45421 caatacttca gttccatttg gtattgaaaa taatccatat atttcatttt ctttaagtgt
    45481 tctccttatc actataatct tactatcttg tactaaatgc tttctcatac tgtttaatct
    45541 atcatcattc attactagta caaaatcacc ttgtatttta tcattctctt ttaaaacttc
    45601 cagataatag tggtttatat caatataatt acctaaaacc aaacgtatat tttcttctaa
    45661 gaaagatgat agtttagtta ttttatcagt tactatagct attgattgtt tcatatttat
    45721 acctcttatt tatatagtat ataattactt atatactact ttatttctac caagttgttt
    45781 tgctttatat aaatttttat cagcttcttc tagtacatct ttgctatctg aaacatgagc
    45841 tacacctgca cttatagtca cagtcacatc atcattccac tttttatttg caattgcaac
    45901 ccttatactg tcaattatat ctgctacttc atcaccagat ttatttctta ttaatacaga
    45961 aaattcttct cctccgtacc taaagacttt atcatcttgt tctaacataa cacaaatggt
    46021 atttgttatt ccctttataa cagtgtcacc aaatttatgt ccataattat catttatgag
    46081 cttgaaatta tcaatatcta tcatcacaag tgcaaaatta tcatctttct ctctgtactt
    46141 ttcatacata atatcaaaac tttttctatt ataaacattt gtaagaccat catgtaaatt
    46201 ttgtgcttta aaatacttca atcggttgta taaaaaagta gaccctaata tagcaattaa
    46261 aattactgta aataatattt tataatattt tatttctttt ttagtttttt caaatagctt
    46321 actttcgttt gatactttat caattatata gaaactagtc tctgaatctc ttatactttt
    46381 gttctcttta tattcattta ttaacatctt ttgatactca tttaaagatt ctatatttcc
    46441 ttgtttttca tatatattta atatcttatt taaatttttt attttctcct gggttaactt
    46501 tcttttggta gatatctcta aacttttttt ataattatct attgcagaat tataattttc
    46561 gcttttatat tctaagtttc ccattgccat ataatagtac atatctttat ctaatatata
    46621 aactgttcta tctgatttta taagataatc agctttttct aaaaaagtct ttgccttttg
    46681 tatattattt ttgcccacat acgcatttga ctgcattata gaggccatta tatcaaaatc
    46741 attgtaatta tccgtattta caaacttttt ataatctttt atcctgctac ttatttcaat
    46801 agctttatca tattctccct cttctacata tatttctgct agatttataa tagcatataa
    46861 tctaaccatt ccattttttt caccttcatc aatatctatt ttaaaagaat cctttattaa
    46921 tttctctcct gtttcatagc cacctatctt taaaaatatg ctcgccaaat caacatctag
    46981 tcttgctttt tcataattat tccccaattt ctcagccaac tttatagact ttacagttaa
    47041 atctactgcc ttagaataat tattatccat tatatacact gatgctaatc ttttttctat
    47101 attaaatcta cattcatcag ataatttcac atctgattct ctcatctttt ctaaagaatc
    47161 aatgactatc ttcgttccat ctggaacata tgaaatagtc tttaacatat cccaaacttc
    47221 attatatact ttattgtatt ttttttctgg aatagattcg aaagaagtct taaccacttt
    47281 ttttgcttca tcatactcct tatctgccac aaggctttca gacatatatt ttcccttatt
    47341 tataatttct ctttctttca atccattttg gtcatacttt ataaacaaaa taatactaat
    47401 aagtattata catataaaaa ttatcttttt gggtaacctt ttctttggta gtctaaaatt
    47461 caaaatttta gtcctccttt tgtatattgt agaatgaaaa cttgcatata taaataacct
    47521 cttaataatt aataatacaa tatatcaaaa tgatttacaa catatgctag acttaaatct
    47581 taataaaaaa gtctcctaaa ataatttagg agactttaaa tttaagctaa tataaattta
    47641 tacagttaaa acaagtgctt gttttttagt tttatattta atatttattt atacatattt
    47701 ttttagtact attgtagcat tatgcccacc aaatcctaaa gaatttgata gtgcatatct
    47761 aatatttttt tctttacctt gatttggaac ataatctaaa tccaaatctt caccaacttc
    47821 ttgtatgttt atagttggag gcacaaaact atcttctata ctcatagcac aaacaacagc
    47881 ctctatagca cctgatgctc ctaataagtg accagtcata gattttgttg atgacacaca
    47941 taagtcatat gccttttctc caaatacagt ttttatagcc atagtttcat ataaatcatt
    48001 gtaataagtt gaagttccat gagcatttat ataatctact tcttctaatg gaacatttcc
    48061 atctttcaaa gccatattca tagacctagc tgctccctct ccattttcag ctggagttgt
    48121 catatggtat gcatcagcag ttaaaccata tccaactact tctgcatata ttttagcacc
    48181 tcttgctaaa gcatgttcta agtcttctaa gataacaata cctgctcctt ctcccattac
    48241 aaaaccatct ctgtctttat caaatggtct tgatgcagtt ttagggtcat catttctagt
    48301 tgacatagcc ttcatgttac agaatccagc aaaagcaaga cctgttacag cagcttcaga
    48361 accccctgct accataatgt ctgcatcacc tctttgaatt accttaaaag catcacctat
    48421 tgaatgagtt cctgatgcac aagctgtaca tacattagtg ttaggccctt ttgctcctaa
    48481 aaatattgaa acttgaccag ctgccatatt tcctatcatc ataggtacaa aaagtgaact
    48541 tactctttta tttccttttt ccaaaagaat tttatgttga gtttctatag cttcaactcc
    48601 acctattcca gaacctatta taacaccaaa acgttctcta tcaactttat ctaaatcaag
    48661 atttgcatct tcaactgcta atttagcaga tactattgca aattgagcaa atttatctaa
    48721 tctcttcaat tcttttttac ttatatattc ttctggatga aaattcttaa cttctcctgc
    48781 tatctgagtt tgatatgtgc tagcatcaaa attagttatt ttatctatac cagatactcc
    48841 agattttata ttactccaga actcttcttt tcctgttccc aacggcgtaa cacatccaag
    48901 tcctgttatt actactcttt tattcatcgg atacctccac ttttttaaag ctcgactatt
    48961 tattagcttc tatatactta actatatctc ctatagattt gaagttttct gcttctgtat
    49021 ctggtatttc tataccaaat tcatcttcaa gtgccattat tatttctact gcatctaatg
    49081 aatctgcttc taaatccttc attaatgatg tttccatagt tatttcttta tcttctactc
    49141 ctaattgctc tattattatt tctacaactt tattaaacat tgtgtacctc ctaaaatttt
    49201 ttattttaat agatttctct actttttaaa ttattattgc attaccattc caccatctac
    49261 atttattact tgtcctgtta tataacttga catgtcacta actaaaaatg ctacggcatt
    49321 ggctacatct tctggtttac cataagattt ctttggtatg tttgctagtg ttgattcttt
    49381 tacatcatca cttagtactt tagtcatatc tgtttctata aatccaggtg ctactgcatt
    49441 tatatttatg tttcttgatg ctaactctct tgcagaagct tttgtaaatc caattacacc
    49501 tgcttttgat gcacaataat tagtttgccc tgcattaccc ataattccaa ctactgaagt
    49561 catatttatt attcttccat acttcttttt catcataggt ttagtaactg cttttgtaca
    49621 attaaagaca ccttttaagt ttatatctat aactctatca aaatcttctt ctttcattct
    49681 cataagcaga ccatctttag ttatgcctgc attattaacc aatatatcaa tttttccaaa
    49741 ttcgcttaca acagaatcta tcatttgatt cacttcatca aatttagata catcacattt
    49801 tatagaggta cactttaccc ccatactttc tatttcattt ttagttttta gtgcttcatc
    49861 ttctttagaa gtataattga ttactacatc agctccaaaa gatgctagtt tttttgctat
    49921 ttcttttcct atacccctag agccaccagt aacaactgct acttgtcctg taagatttat
    49981 cattatatct ctccatttct ataattttgt acaaattcat taagagaatc tataccatct
    50041 atattataaa tattgacatt tgctttttta ttggaagcta tcttcttaat aaatgctttt
    50101 aaagtttttc caggtcccat ttctataaaa gtatcatatc catcatttaa aagtctttct
    50161 atagaatctt cccaaagaac agatgaactt acttgtttta taagcaggtc tttaccttcg
    50221 tttttatagt aatctgcatt tacatttgcc accacatcaa ctttaggttt agttatgtta
    50281 actttttcta attcactggc caattttaca ccagcttctt ttaacattga tgagtgaaaa
    50341 ggtccactta catttaattt aactgccctt tttgctcctg cttctttgca ttttaccatt
    50401 gcttcttcta tcttttcatt ttcaccagat ataactattt gtccaggaga attgtaattt
    50461 gctacttcta caatacctcc ttgtatatct ttaaccacat tttcaagtac atttctatct
    50521 aaacctaaaa ttgctgccat agctccaaca cctgctggaa ctgtttcctg cataaactta
    50581 cctctttttt taactaaaca tacagcatct ttaaagtcaa ttgcatctgc tgcaaccaac
    50641 gcactatact ctcctaagga taatcctaag catgcattat attttaaatc tatttgagct
    50701 tgtaccgctt ttaatactgc tacactatgt gtaaccatag caggttgagt gttttcagtt
    50761 ttagaaagtt cttcttcatt tccattaaac ataaggtttt ttaaatccat atttaaggca
    50821 tcactagcct catctattac acttttagca acagtattat tttcatataa atccttggcc
    50881 atacctacat attgagctcc ttgaccaggg aatactagtg caacttttcc catcttgctc
    50941 ctcctgattt acagttctat tgctggcata atctctcttg cttgatttac catagcctct
    51001 ataatttcct tacaaggtgt tatttctttt accatagcag cactttgacc tgccataaat
    51061 gaaccttttt ctatgtctcc atctactgtt gcaaatctta aagctcctgt acctttttta
    51121 tccagttctt caggagtagc tccattttgt tccatcttta aaaattcttt tgatagttta
    51181 ttttttaatg ttcttactgg atgaccagta cttcttcctg ttacaattgc atctctatct
    51241 tttgctttta gtactaagtt tttatagttt gaatggacag aacactcttc actgcaaata
    51301 aatctagttc ctacttgaac tgcactggca cctaatgcaa atgatgctgc aattcctcta
    51361 ccatctacaa ttcctccagc agctattaca ggtatgttta cagcatcagc aacttgtgga
    51421 actaagacca tagtagtaag ttctcctata tgtcctccac cttcagtacc ttctgctatt
    51481 actgctgtag ctcctagctt ttccattctt tgtgccaaag ctattgtagg tactacagga
    51541 ataacctttg ttccagcttc ctttaatcta tccatatact ttgcaggatt tccagcacca
    51601 gtagtaataa cttgaacttt ttcttctata atcaaatcaa ttatatcatc aacaaatggc
    51661 gacataagca ttacatttac tccaaaaggt ttatctgtta attttttaca ctcaacaatt
    51721 tctttcttta tagcttcttt tggtgcgttt cctgctgcta ttatgccaag tcctcctgca
    51781 ttagatacag cacttgctaa tgatgcagta gctacccatg ccattccccc ttggataact
    51841 gggtatttta tatttaatat tttgcaaatt ttattcatac aattctcctt cacttgaaat
    51901 cataaaagta aactttttat tattaatcaa ctttattttg tgtataagtt taattttgtt
    51961 aattaaatca ttttagatat tttatactat aaagtccact tgacaactga agccccccaa
    52021 gttagacctc caccaaagcc aactaaaact acattatcac cctttttaat tttaccttct
    52081 ctataagctt catctaatgc tactggtata gatgcagcag acatatttcc atatttgtct
    52141 atatttacat aaactttttt catatcaagc tctaatctct tagaagaagc ttgtatgatt
    52201 cttatattag cttgatgtgg cactaagtag tctatatcag ttgtatttag atttgctttt
    52261 tcaagagctc ttaaagaagt ttctgccatc tttctaactg caaatttaaa cacatcacta
    52321 cctgccattt ctattgtatg tagattagct tctacagtct ctttacttgc cttaagtctt
    52381 gaacctccag caggtacttt taaacaatct tttcctttac catcagagcc catatgtgta
    52441 gacagtattc caccctcatt tacagggcca ataacagctg ctccagctcc atctccaaac
    52501 aatactgctg ttgttctatc atcataattt aaaaacttag aacaagtttc tgctcctatt
    52561 acaagcacat ttttatagca acctgtttcc acaaattgct ttgcaatagt tactccatat
    52621 acaaatccag aacaagctgc tgatatatca aatgcagtag cattcactgc tcctattgca
    52681 tcttgtacta tacatgcagt tgaaggcaaa gacatatcag gagtaattgt agctaatata
    52741 atcagctcta tatcctctac gtttaacttt gcacattcta tagcttttaa ggcagcttta
    52801 gatgctaagt cagaagttgc ttcagactct ttggcaaatc ttctttcctt aataccagtt
    52861 ctagtactta tccactcatc tgatgtatcc attatttttt cgaagtgaaa attatcatat
    52921 gattgctcag gtagataact tcctactcct aatattccag cctttgtatt cattatatat
    52981 taaacctcca aaacgagttt attcatcttc gttattttta ttttcttcat tgtatttgct
    53041 tataaattgg ttaatatctt caacgacttt tccctttgaa aattttattc cttgatttat
    53101 agcatttttt atagctttag catcagaact accatgagct tttataactc ctccatttag
    53161 acctaaaagt ggagctcccc catactcaga ataatctata aaacttttta attttcttaa
    53221 atcatccttt ataagtaatg ctcctatttt actttttgta ctggccataa aagtttcctt
    53281 tataagactc ataactgaaa gtgctacacc ttcagcagat ttaagtaata tattacctgt
    53341 aaatccatca catatgatta tatctgtgta tgcatttata acttctctag cttctacatt
    53401 ccctataaaa tttaaatcta attttttaat ttcttcatat gaccttttga ctaaatcatt
    53461 gcctttgcct tcttctagtc caacatttgc taatgctacc ttaggatttt caagacctaa
    53521 aacttttctt gaataaatat tactcatagc tgcaaattca actagattct taggtttaca
    53581 atctgcattt gcgccacagt ctgctataag tgtcattcct cgctttacat ttggtattgc
    53641 tgaacataag caaggtctat cgattccttt tattctacca acaacaaata atccaccagc
    53701 aagtaaggct cctgtatttc cagctgatat tatagcatct gcctttcctt cttttacaag
    53761 atttaatgct actaccatag atgaatcttt tttacttctt atagccttta ctggtttatc
    53821 ttcgttttct ataatctcag tagtatgaac tatctctaat ttatttctat caaattcata
    53881 atttgaaaat tccttttcaa gtaaatcttt atctcctgtg attattaaat caacctgata
    53941 ttcttttata gcatttactg cgccttctac attagattta ggtgcattat ctccacccat
    54001 tccatcaatt actattttca taatttccct cctaatctaa ttcttcaaag ataaactttc
    54061 ctctaaatat ttgctcttgg gctttattgt tgataacaac ttttacataa tgctttttat
    54121 ctatatttct atacacttct gcttttgcta taagtctatc ttgttgttct atcaacttaa
    54181 aactttttac atttgcaact ctcataataa ccttaggtgc gtctattata gccattgcca
    54241 atgaatctgc tagagaaaaa atatacgtat cttttaaagt attagtctta gagtaaatca
    54301 tgtcctgtgt aacttctaac atagatatac ctaatcgccc tacagataaa tcaatgattt
    54361 ctcctgtaat ttcctttact cctaaagtct ttacttttga actctgagtt tctgctattg
    54421 atttgactct ttctcttagc tctggtatgt ttagactcat tctatctaat cttatggtct
    54481 gtatacttac atcaaacaga cttgataatt cttcatcagt ataaaaagga tctgtcttta
    54541 gcatatctat aagctctttt tgtctttggg ctttactttt ctttttcatg ttccctccaa
    54601 attttatagc tgtaattagt accagctact aatagtagta tatagtaaca cacaaaaatt
    54661 ttcaatactt atattattat ttttctattt tttaatatgc ataaaatcca cttattttct
    54721 ctagtaaatc atcttatttt tttatatgta caaaaaaaac atgtagagta tactctacat
    54781 gttttttaga agctattact tagacacaac ttctttacct ttataatatc cacagtctgg
    54841 acacactcta tgtggtaatt ttggctcatg acattgtggg caacttacaa atcctgttgc
    54901 ttccatcttt gagttagctg ctcttctcat ttttgtgttt gatttagacg ttttacgctt
    54961 tggtactgcc atttacgcca cctccttagt caaaatttaa ttaaataaat cttttaattt
    55021 agcaaaacga ggatctatgt attcctcatc gtttgtaatt tcactgcaag aacactcttc
    55081 ttcatttaag tttgccccgc atacttgaca aagaccttta cagttttcac tacaaagaac
    55141 tttatgaggt acattaaaat ctaatgtctg ttctattata tcaataaggt cgatttcttc
    55201 accatcgaat ataaaaggat caaattcctc aaactcatct tcatcataat cttcttcttt
    55261 tactaaaaaa ccttgtatag aatactctag tggtatttct acttctttaa gacatcttga
    55321 acaattgtca acaattgtaa aattgacatc tatatctaga tacaaacctt tattagtctt
    55381 tgacactttc ccttttaaat ttacaggtga tactaatttg taaatctcat cacaataact
    55441 aatagtatca attttttcac aaaaattcaa gtctattttg tcagtttctt ttctatttat
    55501 tttttctatg ctaactttca ttaagttcac ctcaacatat caccaaattt attatacaaa
    55561 tctagcattt gtttgtcaag tatttttact tgataggcat aggtttaatt taattatttt
    55621 accaatctta atgtatctct agctatcatt aattcttcat tagttggtat taaaagcatt
    55681 tttactttag aatcagcagt agatattact gtttcttttc ctctaacatc attagcttct
    55741 ttatctaatt tcattcctaa aaactccatg ttagaagcaa tatctgctct tatagccttt
    55801 ccattttcac caacgcctgc agtaaatact acaacatcca atccattcat ttcagcagca
    55861 taagcgccta tatatttttg tactttttta acatacgctt ctaatgtagc ttgagctctt
    55921 tcgtctccat ttttagctgc atcttctata tctctgaagt cactagaaat tccagtcatt
    55981 ccatatactc cagattcttt attcattaat ttatccaatc catcagcatc taaaccttct
    56041 tttctcatta agaatggtaa tatagctggg tctatatctc cacatctagt tcccattatt
    56101 aatccttcta atggagtgaa tcccatagat gtatctacac attttccacc atcaacagca
    56161 gctatagaag caccatttcc taagtgacaa gttactattt ttaaatcttt tatatctttt
    56221 cctaatatct ctgctgctct ttgagataca taattatgag aagttccgtg gaatccatat
    56281 cttcttaccc catatttagt atatagctca tgaggtaaac catataagta tgaagattta
    56341 ggcattgttt gatggaaagc agtatcaaat acacctacca ttggtacatc tggaagtata
    56401 gctttacaag catctattcc cattatgttt gctggattat gaagtggagc taaatctata
    56461 cattctttta aagcttcttc tacttctgga gttattacta ctgaagttgc aaatttttct
    56521 ccagcatgta caactctatg tcctactgca tctatttctt tcatttcttt aactgcacca
    56581 aattctggat gagcaacagc ttctaaaact aatgctattg catctttgtg gtccttcatt
    56641 ggttgttcaa caacgtattt atcatctcta cctgcttttt catgttttaa tatagaacct
    56701 tctattccta ttctttcaac caatcctata cataaaactt cttcattatt catatctatt
    56761 aattgatatt ttaatgaaga actcccacag tttaatacta atattttcat tattatttcc
    56821 tccttagatt tttttataaa taatatgtaa taaattttga tttgtaataa atgtctaaat
    56881 aaaactctat cttttattat tttgttatag atatattatt gtatacatta aacgattttc
    56941 ataaataata tatcaccttt taatataaaa gtatatactt tttttttata ttatatcata
    57001 tttctagtat ggtaaattgt gtatttcact aataatatat taacaagtta acttaatgta
    57061 gattattctt tcccatttgt tataataata taatataaag agctaataaa gttattttaa
    57121 taacatttaa ccagtttttt atgtaggtaa catttctaca tttttataaa tttttttacg
    57181 ttttttaatt ttataattaa aaatttaaat tttatacata ttataataaa ttgtgatata
    57241 tataaaataa ttaaagataa aaggagtatt ttatgaacat acttggatta atagttgaat
    57301 ataacccatt tcataatgga cacatatatc atttatctaa atctatagaa aaaacaaatg
    57361 caacacatac tgtagcaata atgagtggaa attttctaca aagaggcgaa ccagctttat
    57421 ttgataaata cactagagca aaaattgctg tcgaaaatgg tgtagactta gttatagaat
    57481 taccaacttt atttgcttgt caaagtgctg aaattttttc tcatggtgct ataacaattt
    57541 taaattcact aaactgcata aattctatat gttttggtag tgaagaagga aatacagaaa
    57601 ttctttataa tatagctgaa attttagcaa atgaaccaaa tgaatttaag ttacttttaa
    57661 aaaaatattt agatgatggt ctgttatttc caacagctag agctaatgca ctttttgatt
    57721 atatacatca agacaaaaaa aataatttta tacaaaatta ttcaaaggaa caattatcaa
    57781 aaacattaaa ttcatcaaac aacatactgg gtatagaata cataaaaaac ctcatccatt
    57841 taaacagtag tataaaacca tttactatct ccagggtcca atcagaatat aattccgaag
    57901 aaattgagag tgatatttgt tctgctactg ctataagaac ttcacttaaa gaaaatacag
    57961 acctttttca tttaaaaaat gttattccag aaaaaactta taacatccta aatgaaaaaa
    58021 tagataatgg ttttttacct atgtttgatg actttttctt tgatatttta aaaacaatta
    58081 tacttagaga tatggacact ttaaaagagt attttgaagt aaatgaaggc atagaaaaca
    58141 gaatctataa agatatcttt acatctgaat ttctacatga actacaatta gcaattaaat
    58201 ccaagcgata cactttaacc aaaatcaaac gaactctaaa caatatattg ctaggtatta
    58261 aaaaaagcga catgcaactt ataaaaaaca tagatactat accttatata agaatacttg
    58321 cttttaatga taaaggtaga gaaattctaa aagagataaa aaataaatct gaaataaaaa
    58381 taataaacaa gttttcaaat atatcatttt cattagatga tacgattttt aaaacactca
    58441 taagttatga tataaaagct acaaatatat ataatttaat atattataaa aacaataaag
    58501 atttagtaaa aggccctatg gatttttata ctcatccaat atatgtaaag tagtttttta
    58561 aaataactat atttataaaa atcattttat taactcttta ttatataaga tacaatagtc
    58621 ttactttatt tatgtacaca tgcttttagg atgacaacat caacttaaat attatgttac
    58681 tttctaaatt taagtaaata ttgccatcct aaaaatttta caatttagct tcagatttaa
    58741 aagtaaagtt taaaatatta tatttaaatc taatttaata ttttttgttt tgtaaatatt
    58801 attaaattaa tatttctaat atattttccc tggatttaaa atatttttag ggtcaaaagc
    58861 taactttata tttttaatta gcatcatata aacatctgaa tttgattcat gtaagtactc
    58921 ttttttagca tatcctatgc catgttcacc agaaacttga ccactaagtt ctctagattt
    58981 tttatacata tagtcaaaag tttcttttaa tcttattttc catgtattat catccatacc
    59041 atcttttaaa atatatatgt gtagatttcc atctcctgca tgaccaaaac tttttattct
    59101 tatttttaat ttgtcttgaa gctcgtgagt gtatcttata aactctgcta tcttatctct
    59161 tggaacaacg acatcacatt catccatttg tgtcgtagat gcctttatag cttctaaaaa
    59221 tgcacctctg gctgaccata tagaatcgtt tctttcttgt gtatctgata taaatacatc
    59281 taatgcacca ttttctaaac ataaattagc tactttttca tattcttttt ctatatcttc
    59341 agtgctattt ccatcaaatg taagcaaaag ataagcatct gatgtattat ctggaaactt
    59401 tttacctaaa aattcttctg ctgccaatat tacatctctt tccataaatt ctatagccgt
    59461 tggtattgat tttgatttta ttattttagg aacagtttct attgccattg ataaatctgg
    59521 aaatggtata agcaaactta tacttttctt tggtaaagga agcaatttta atatagcttt
    59581 agtaactatt cctaaagtac cttctgagcc cactaataaa tcctttatac tatatccaga
    59641 actattttta actacctttc cacctacatt tataatttct ccatttggta gtacaacttc
    59701 aagacctctt acataatccc ttgtaacgcc atattttact gctctcatac cacctgcatt
    59761 agtgtttata ttacctgcaa ttgtagcact tttttcacct gggtcaggtg ggtaaaataa
    59821 atctctatct tgaacatatt gacctattgt catcaataaa acacctggtt ctactgtcaa
    59881 tgttaaattt tcataatcaa cttcaagtat tttattcatt ttacatagat taatcattat
    59941 tccaccattt atggcaacag cagcacctac aagaccagta ccttgacctc ttggtgtgac
    60001 tggtatatta tttttatatg cgtatttcat tattttagat acttgttcag tttcaagtac
    60061 atttactaaa acttcagggt acttctcaat ccctccaagt tcatcatgtg aaaaatcttc
    60121 atttatatca cttccaacta atacattttc ttctccacaa gtatctatta aaaaatctat
    60181 atctttttta tcgattaatt tatacatttc tatcccccta tttagctgct gatgccatat
    60241 atttttgatt attatctttg ttattttcta ttttttcaat caatttagga ataatatcat
    60301 aaatatcacc tattaaagct aaatgtgcta catcaagtat aggcgcttta tcatctttat
    60361 ttattgctac tatatagtca gcaccctgca tccctgcaac aaattgaaca gctccagata
    60421 caccacaagt tattatcaat ttaggtttta cagttctacc actaagacct atttgttttt
    60481 tagcatctat ccaacctgct tctattacag gtcttgttcc tgccacttga gcgtttaact
    60541 tatcagctaa tttatatatc atctccatat cttcttgttt ttttattgct ctacttgcca
    60601 caactatcac ttcagcttct tccagaccaa cttctttatt tttttctttt acactcagta
    60661 ccttaatttt agattttagt tctttttttt ctactttaca taaagttatc tttcctgtag
    60721 tattctctat tttttctggt gctgaaaata ttttatatct tacagtggca aattgtggtc
    60781 tattattagg tgtgtttata tgagccatta tatttccacc aaatgctggt cttatttggt
    60841 ctaaatcagt atttgattga atatcaagta ttgtacaatc tgctgtaaga cctgttctaa
    60901 atctagcggc aagtcttgga gctaatgacc tacctaatgt agttccacca actaatacta
    60961 tcgttggttt tattttatct ataaagtttt ctattgcttt tgaatatggt tctatcctaa
    61021 aatctttaaa ttcatcttca tcatatacaa atacttcatc caccccataa gaaatcaaat
    61081 tactgcactt atcttttatg tcttttccta caaatacaca atacacaggt tgttttattt
    61141 ttgatgccat ctcacgagct ttacctataa gctcgtacgt tacaggatgt atatttccat
    61201 tatgatgttc aacatatact gcaattcccc tccattcatc tttattaatt tgtactttac
    61261 tactctctat aaattcaaat gcgccttttg gtccactttt gacacacaac ttgcacatct
    61321 tacagccagc acttatttct actatacctg acttttcttc tatagcccca aaaggacata
    61381 tttctaaaat tctctttaag tcagttattt tatcttgatt tacaactatc tttgacattt
    61441 attactcccc cttacaccaa ctttaattcc tctaatttat ttacaagtgc aaaacttagt
    61501 tcatctgcat ttcctcttac aatattttta tctgtatttg gctttggagg aaatattctt
    61561 acaacttgag taggagaacc attaagtcca tagttatttt catttttatc ttcaaagtca
    61621 tctaagctat atacaggtat ttctctatct ttagtattaa gttttaattt aaatgaaggt
    61681 agtcttggtt gaaaaatgtc tttttctaca gttattaaac atggatattc aatctcacat
    61741 acttgtaaat catttggcat atctatttca acaactatac tttcatcttt aacttcaatt
    61801 aattttgtaa cgttagtaac atgaggtata tctaaaaatt cagctacttc aggtcccacc
    61861 tgtgctgtat caccatcagt agtttgtttt ccacaaatta ttaggtctac ctttcccatc
    61921 ttcttaatac cttgtgatat ggtataggaa gtagccaata catctgctcc tccaaatctc
    61981 ctatcactta ttaatgctcc ttcatcagct cccatcataa aactttcttc aattaccttc
    62041 tttgcctgag gaggacccat gctaagtact tttagattcg ctttcttctc ttcctttatt
    62101 ctaagagctg tttctaaagc atataggtca tagggattca tttttgaatc tactccatct
    62161 ctttttagta ctcctgtagt ttcatcaact tctactgaag tcgttccagg aacttgttta
    62221 atacatgtca aaatttccat aaagtacact ccttttttat ttaactataa ctgatactcc
    62281 atcaggtata actactactt tgctctcctt accaactata ctattcgcca tttctaaagc
    62341 ttctttaaag ttaaatgcat gtttcatatg catttttttt atcatttcag gattacactt
    62401 atctgttacc attatcacag tatatttaga taatattctc gctagtatct gaaattccca
    62461 ttggtctgga atcgtatcta ctctatcgac acttctaatt ttatctaaaa gttgttttgg
    62521 agaatctgca tttgctacat tttcataaaa agattgacct ccatgaccat cattacatgc
    62581 tgaaatcatt attattactc caccgtcttt acaagtagct tcagctgctg tcattccttt
    62641 aacagattga taaatatttt ggtctagtgg atatccacca tttgtagtta caacaatatc
    62701 agattttata gaatttactt ttgataactc tgttacaaat ctacatcctt tagtatgtgc
    62761 tttttcgaca tcaccagcaa aggaagcaat tattttttta tctttatcta ttacaacatt
    62821 caatatgaat gctaattttg caactttagc tgcatatacc atatcctcat gaataggatt
    62881 attattcaat tttccagttc ttgaattatc actatctata aagtcactac agtgattgta
    62941 cattatagtc tttgcagatg cgattcctgg aagtacgctt tttctgccac ctgaaaaacc
    63001 tgcaaagaaa tgaggttcta taaaaccttc tgctatcaaa agttctgcct catatgctag
    63061 tttgtttata tataaatctc cacctgaagg caatatccct actttacaca tagatttatc
    63121 atcagtagat atatgcataa ctatatcttc attatctact atttcttctc ccattttata
    63181 aattagctct tcccttgtac ttggcctatg aaatccagtt gccactataa tcttcacatc
    63241 aatttctgga ttaacattcc taatccttct taatataatt ggcatagtta ttctacttgg
    63301 tacaggtctt gtatggtcac ttgtaattat gactacattc tttttcccct taaccagttc
    63361 ttccagagaa ttagagccta taggattatt catagattct tcaactatct cttcttgagt
    63421 cttatgttct atataattat ccgattttga ctccaaaaca ccgagaaaac gttcatcagg
    63481 tatttctaaa atcattcctt gttttgaata cggtactttt aactttacca tttttaatct
    63541 cccattataa taaactataa aattggtatg tggtcatacc agttttacat ttatatgata
    63601 gtaccattat ctaccatttg caagaataat ttattttata acaaactttt aattttcatt
    63661 ttttctataa tttttactta taaaatccat atgttccttt gctttctcac atgcaagttc
    63721 tacatcattt tctacaactg cattgtatat agcttcatgc tgtttaagca atatattttc
    63781 attaaaaggt tctgcgatta ttttttctct tgcatcttct ataaatccat caaataattt
    63841 tgatgctgtc ataaaaagac tttgaattaa ataattttta cttaatgttg ctagtttact
    63901 gtgaaatttt tggtctaata acaccagtac tcgattatta tcttttttat tagtttctac
    63961 cctcatttca tctattatat gtttcaattc tataacttca gaagaagtta ttctctttga
    64021 agcaagtttt gcaatctcaa tttctaggat tattctaagc tctaatatat cttcaagtgt
    64081 accattatta agtttaaaaa tcatagataa aggttctatt aaagtttttt ctatatttgt
    64141 acatataaaa tttccttcac cctgtctgct ttctatcact cccatagtct ctaataccct
    64201 aatagcttcc cttatggaag ttctactcac attcatttgt tcagacagtt ctctttctga
    64261 tagtaattta tccccctttt ttaactctcc atttagtatt ttatattgta tttgctcaat
    64321 tacttgttca taaacttttt tactagctat actattaaac attttttcct ccaatatgat
    64381 aatcgttgtt gtaatatata tctattatat atcttaagcc attacattca tatattttta
    64441 ttacttttaa tataatatct tataactcaa atttacaggt gatattatga aaaaaaataa
    64501 aattattgct ttttttgctc catctatcat agttggagtt ctagttttag ggattatcct
    64561 atacccaaat gaagctataa aatctgcaag agaaggcttt tctatctggt ctaatgtttt
    64621 agttccatca ctactaccat ttataattgg agcaaactta attgttgatt taaaaatagt
    64681 ggatattata gggtttatca tcaatcccat aactagattt gtatttaatg tttcaggaaa
    64741 gagcgcttta gcttttgcta tatctacagt atctggttat ccagtaggag taagtttagc
    64801 ttctgaattt aggagtaatg gacaaatttc taagtatgaa gcacaaagac ttgtgtcttt
    64861 ttgttctaca tcaggacctt tattcataat aggttcggtt ggtacaggta tgttccaaaa
    64921 ttcaaactta ggttatttaa tgattctctg ccattatcta ggaactatat tggttggtct
    64981 tttctttaga aattatggta atgaaaatct tccaaagaca aaattggatt taaaaacaaa
    65041 tataaaaaat gtaatcaaca ttagaaattc aagtgggaat ggtttttttg ttttatttgg
    65101 aaatgctgta tttaatggag taaacacatt acttacagtt ggtggatttg taattgtttt
    65161 ttcagtagta tttaaaatac tatctctttt taatgtaatc tcattaatag catcagttat
    65221 atatttgcct ctttcatttt taggtgttag taaagaactc tgtcaagcat ttgtaagtgg
    65281 tctatttgaa attactatag gatgcaataa agtatctagt ataacaagtt ctccagaaat
    65341 tttaagagca tctttagcta gttttttgat aggatttagt ggattatcca tcctagctca
    65401 atgttgtaca tttctatcaa aaacggacat agatttaaaa ttatacatac taagtaaatt
    65461 tgcgcatgga gttttagctg ctatctttat atttatactt tatccaattg caaactctgc
    65521 tgtgttggtt tctagctttg ctgatacata caacgttata tataataatt taatctggtt
    65581 ttattacctt agctattatg atacaattct tcaaattgta gtaataattt atctaatctc
    65641 agccgtattt attgcaaaaa aacatatgaa aactttatct acaaatggga aactaatgcg
    65701 atataaaaat acttttttca ctaagaaatt ttcacgaaaa aaataaaact aaaaataaaa
    65761 ctaaaaataa aactaataaa acttaactaa atacttccat ttttttatta tatttgtcat
    65821 aattttaata acctttttaa ttagtttaac agcatagtta tttcatcaca atttaataat
    65881 tttattagaa atatttttca aaaaattctc cttaatcaca tatatcacac ttgtataagt
    65941 ttactgggaa atttttccat tatataatat ttcatttata ttatataata aataccgacc
    66001 taaacttacc aaatcaaact aagaggagtg gttgaaaatg gcaacaagac ctatagaaat
    66061 agacgaatat aaaaagataa tggaactatt acatactggc tttacttata gtgaaaatgg
    66121 cgttgaaaaa agatttagaa aaaatcctaa agttgcattg gcactaatgt tagaagctaa
    66181 tttaggtcta agaatatcag atattttaag attaaagata ggtaatttta aaggtaatat
    66241 gttggaaata aatgaaaaaa agacgggcaa attacagtat agaccagtta ataaaaatat
    66301 aattgagtcc ataaatgaac atgcaagaaa gtataatctt aaatcaaatg actacttagt
    66361 aaatataaag acaaaggcta ttcaaaaaca gttgaggata atttgtaaac acttaaatct
    66421 gtataatata agtacacata gctttagaaa gctctatgct actactcaat ttgaaaagag
    66481 taacaataat ttagagttgg ttaaagaact tttaaatcac agctctgtag ccactacaca
    66541 gcgatatata agggtaactc agcaagctat taatgaagct agtgaaaatt tctttattgg
    66601 ttaattataa cgtatacatt tttctattat cttattaatt caccagatat taaaaaagag
    66661 tgtctcaaaa tgaacttttt agttcatgag acactctttt tttataaaat caaatatatt
    66721 tttattattt caacactgga aaaaaggctt atctctctat tttgagacac cctattaaat
    66781 tttcataatt cttgcataaa catattaatc tatcttcaat aattttttag ctgattcact
    66841 gtaattattt ctttttacaa tttccaaaac tctatcaatc tcatgttctg actttgcact
    66901 tacaaaaatc gccaattctt catcatctat aatatcataa ctatctacat atttatctaa
    66961 acattctaaa attccatctt tattattata cccaccaaat ctaactactg cactacactc
    67021 atcttctata agtccactta aatccaattt cccttttgaa aaagacttta tatttgaact
    67081 tctattatct attatatcta taatatctgc ataaacagca cttcctgttg cacctcttcc
    67141 agctccctta cctacaaaag aaagttcacc tactgaatca cctactaaaa taactacgtt
    67201 gaactcatta tctatctttg aaagcatatt attattgtcc actaatgctg gtctaacaaa
    67261 tccacttact tctcctgatt catacttact tctagcaacc aattttatct tacaatttaa
    67321 tttttttgca tattctatat ctttttcatc tattgaagtt attccttcta ataataaatc
    67381 tttccaatat actatttttc cataagataa agtagacaat atagataact ttctagcact
    67441 atcatatcct aaaacatctg catctggatt aacttctgca aatcccaact cttgtgcctg
    67501 ttttaaagcg acatcatacg gtaaattttc atcatacatc tttgataata taaaattaca
    67561 agtcccattt aaaatagcgt ttatgctctt tatattgttg ccttccagtg attctatcaa
    67621 tggttttaaa actggaatac cacctgccac agatgcttca aacttgatgc taacattatt
    67681 taagttagct aattctataa ggtcactacc tttttcagcc agtaaatctt tattagctgt
    67741 cactacatgt atttttttat ttaacgctct tgttacataa tcaaaagctg gatttaatcc
    67801 tccaattact tcaactagta tatcaatatc cttgtcaaat atatcttcta tatcagtagt
    67861 tatcttatca aagtacttct tatacttgtg tttttcttta tttttaacta aaattccaac
    67921 aacttctata tctctttttg aagtattatt ttctattaaa tcaattaatc cacttcctac
    67981 tgttccatac cctaatattg ctatcttcac tgtaacatac ccccatcatt ttgattatat
    68041 taaattattt atatatataa atataccaat aaaaaaagct tgagtataaa ctcaagccat
    68101 atgacgggtt tatactcatc tttcagcata aatgctgcag gatttagcac caatgtataa
    68161 ttaaatatac tggttgccgg gcttcacagg gccagtccct ctgccactct tgataagatt
    68221 gctaatttaa atttatgttt aatagtataa tacaatttct tccaattgtc actatttttt
    68281 taacttattt ccctaaaagg atataaacta ttatctaaca gctcttttag ttggtttacc
    68341 cttttttgta acattattat ttttagtctt tgctttcgcc tgttcatgtc ctggaaaaag
    68401 tattttaggc tcttcaatat tcctcttgta aatagtaaat ttaaaaccaa tagcctgcac
    68461 aaattcagct cttaaagctt cacatactgc attagcagtt tctttagtat caagacctgc
    68521 attttctaat atagtaactt ttactaattc tctagttctt aacataccat ctaattgctc
    68581 taagaaactc tctgtaacac cttctttacc aatttgtgta gtaggcttta aagtatttgc
    68641 caaagatctt aaatacgccc tttgttttcc ttttaacata ttctcacctc tataattcta
    68701 tacttaaatt atctataatc tatatttaaa ttaattgtaa aactcaaatt ctaattcata
    68761 aattttaacc acatctccat cttctattcc catttctcta agcctatcaa aaacaccttg
    68821 actctccata gctttttgga aatattgaag ggattccata tcatcaaaat ttacagaata
    68881 cattattctt ctaagtgctt tacctgtaac tacatacaca ccatcttcaa tatctatact
    68941 taactcttct tcagtaccaa tatcaagttc tggtctatac atttcttctt ctgaaactag
    69001 ctccacatct tctacttctt ttagcatttt agaaacatag gctattactt catctatgcc
    69061 ttgacgagtt gctgctgaca ttttaaatac tgtatatcct ctgccttcta attcactctt
    69121 aaacttctca aacttactct catcttctaa tatatcaaat ttatttgcaa ctacaacttg
    69181 tggtctcttt gaaagctttt cattgtacaa tttaagctca tcatttatct tgtcaaaatc
    69241 ttcaataggg tctcttccct ctaatccaga tatatctaca atatgtatca atacctttgt
    69301 tctctcaaca tgtcttaaga actcatgacc taatcctatg ccttcagaag caccctctat
    69361 aattccaggt atatctgcaa gtacaaaact atctccaaac ttagtttgaa caacacctag
    69421 atttggtgta agtgttgtaa aatgatagtt tgcaattttt ggtttagctt tagttacaac
    69481 tgatagaaat gttgatttac ctacatttgg aaatcctaaa agaccaacat ctgctatcat
    69541 tttaagttca agagttatcc atctttcttc tccatctgta ccagattttg caaaagctgg
    69601 agcttgtctt attgcatttg caaagtgttg gttaccttta ccacctatac caccttttgc
    69661 aactatagct gtatctcctt ctttttttaa atctgctaat acaagacctg tagcctcatc
    69721 tcttattact gtacctgctg gtacttttaa tactaaattt tctccatttt taccagctct
    69781 ttttcctttg cttccgtcgc caccattttg agcttgatat tttttctggt acttaaagtc
    69841 cattagagtt ctaagcccca aatcaacttc aaatatgatg cttgctccac gaccaccatc
    69901 tccaccgtct ggaccaccag ctggaacgta tttttctctt ctaaatgcta ccgaaccatt
    69961 tccaccattt ccagccttaa caaatattct tgccttatct ataaacaaat tcttcaccta
    70021 cttaattaaa aacttttaat tagcattacc aaaaatacca ttataaattc atcagtatta
    70081 taagaaaaag gagtgcctca acactccttt tatgtatatt actcagctat tgctactgga
    70141 tatatactaa ctttttttct cttcttgtct tttctttcga atttaacagt accatcaact
    70201 aatgcaaata aagtatcatc actacctttt ccaacattag ttcctggatg tattttagtt
    70261 cctctttgtc taactattat acttccagca gttactaatt gaccatcgaa tctcttaaca
    70321 cctaatcttt tagatataga atctctacca tttttagaag aacctacccc ttttttagat
    70381 gctaataatt gtaaattcat gtttaacata tggagtcacc ccctactttt ttaaaatttt
    70441 tatatttttt gggtattccc gtttaatttc tttaaccgct aattgaaagt gatttagtaa
    70501 taattgtgcg tcttttacat gtgtatcttt gactatacac tctatatgac cttcttctcc
    70561 tactactttt tcaaattcaa tcttttgtaa tttgtctaaa gaatttataa ctgcaatagc
    70621 attagatgta actgcagcac atactatatc atagcctatt tcagcaaaat cagcatgtcc
    70681 ctttaaacaa aaaccttgca acaagaaatc atcattataa taatatttaa cttttatcat
    70741 aacttaacca ataactaagc gtttattttt tctacaacta tcttagtgta tgattgacga
    70801 tgtccttgtt ttcttctgta atcttttttt gctttatact tgaaaactat aactttctta
    70861 gctttacctt gttcaacaac tttagcttga acagatgctc cttctactac tggagaacct
    70921 aattttaatt ctccatcttt agagcaagct aatacttcgt ttaaagtaac aacatcacct
    70981 gcgttagctt ctaatttttc aacgaataat acatcacctt cagaaacttt atattgcttt
    71041 ccaccagtct ttactatagc gtacattaaa tacacctcct cggcctcaaa ctcgccagta
    71101 caaggtactt catttgaaga tttaaacctt tactgtgcgg cattacaatt attaattata
    71161 aaatatttac attttattgt caactatttt atcgaaaata acattggttt tattgtgctt
    71221 taatgtacta tttgcgttta aagaaatctt tatattatat ttttcaccaa ttttatcaat
    71281 tatatccata taatattctt ttatttgttc aaaaataaaa tcattaaatt ctaccattat
    71341 gtttttgtaa acagtatgtt cacttattct cataatttct ttttcaatac tatctagtat
    71401 ataatgtaca gattttattg tttgttcacc atcacaacat ggacaagaca ttaaataata
    71461 cttatcaatg gattcttttt ctctacgcct agcaacttct actaaaccaa gttttgtcat
    71521 acctaatact tcagcttttc ttttatcttt attaaattct tcttctagga tgtgtaaaat
    71581 ctccttttga tgcttctttt tatgcatatc aataaaatcg attattatta tcccagctat
    71641 gtctctaatt ctaagctgtc ttacaatttc ttttgcagct tcaagattag ttttataaac
    71701 ggtttcatca agtttaccag ttccaataaa cttacccgta tttacatcta taaccgtaag
    71761 agcttctgtt ttttcaatta ttaaataacc tccactcttt aaccatactt ttttatttaa
    71821 taatttttct attttagatt ctattctgta taaatcaaat acatctctat tttcctcaag
    71881 acacagttta tcaacataag tcttatctat acctcttaaa atagacttaa gttcagagta
    71941 cttatcataa ctattggtaa ttatagattc aatatcatca ttcacattat cttttacata
    72001 tttagttgca aaatctaaag acttatataa taatttcgga cctataccaa gtttatattc
    72061 ttttaaaatg ttttcatact ttaatttcaa ctcttctata tctttcttta tttcatcatg
    72121 agtgcaacct tgagcctcag tccttattat taatcctaga ttttccttat ttactgcctt
    72181 agtaattttt tttaatctaa atcgttcttt ttcatcagta attttatttg atattgtagt
    72241 tctatcattg gaaggtatat agacaatata ccttccagaa atactaatct cagtgtttaa
    72301 ttttgcgcct ttagttccta tttcttcttt atttatctga accaaaatat cctgaccaga
    72361 ttttatagta cttccctttt ttaattgtaa ataagcaagt ttcttaaatc caatatctac
    72421 aaaacaagct tctattcctg gtataacttt ttttacgatt ccacgatata tattggatac
    72481 agtcttctta tttaaattgt cttcaacaaa aagttctgtt aatctttcat cttcaagtac
    72541 tgctgttttc tgggacccta ttagggactc aataactatc tttttcacaa cttcacctct
    72601 cgagcttaca taggagtcac taattcccca tcttctaata catataaatc tcttctcaat
    72661 atatcaacat ctaatgggtc aatatcaagt tcaaacatct ctaatatctt tggtataagt
    72721 atattagtat ttaaattagt ttttgagcct gtagctatag tagcagtaag agtaatatgt
    72781 tcatcttcta aatttaaaac atcaaaggtt cttatcatag gtcttatatc tacttcaacc
    72841 atcttacctt ttttattctt ttttgttatg attatttctt tattattcat aaagtcaatt
    72901 acttttcttt ttacaaactc tttcgtcata ggtcttttca aatctatatt aaacaaatat
    72961 tctccatagt ctatcacaga agaaagagaa ggtgtttgtc tatcaatttc ttttgctttg
    73021 ataaaatcta tcccatctgg aagttgaatt gatactttat ttaaaaattc ctctactgat
    73081 aaatcatctt cttctatttc aatatctaca tactcacctt gactttctgt ccctaaagcc
    73141 aaagcattgc cataactcat tttagggtga ggattaaatc cttgagaatg tgataagttt
    73201 atttcagctc ttctaaacgc tctttgtaac aatctctgaa ggtcaagatg tgatatatat
    73261 atcatgtcgc cttccttttt aaattttact cttattatct tactcatatt agcaaagccc
    73321 ccttcctata tcatgtatat ttattccaca accattgcag ttatgtctac aatcagaagt
    73381 gattttttct tttttagcat tttcgtcttc tctaattaag aattttttac taatacctac
    73441 atctatatgg tcccaaggaa atacctcttc atagtttcta tttctatgtg cataaaaatc
    73501 tatagaaaga ttattctttt ccatagcttc tttccatata tctaaattaa aatgttctgc
    73561 ccatccatca aatttagcac ctagtctaaa tgcatcatat ataacttttc ctattcttct
    73621 atcgcctcta gctactactg cttccatcaa actagtcttt gaatcatgat aattgtattt
    73681 tatattgtta tttcttaatt tatttactaa atgtctctgc ttattaataa cttcttctgt
    73741 agtatcttga ccatgccatt ggaaaggtgt aaatggcttt ggtacaaatg tagaagtact
    73801 cactgtaaca ctaaaacttc tctttagctt tcctccattt acttttctat aaatatcaat
    73861 aactttataa gctaatttag ctataccatc taaatcttca taagtctcag ttggtaaacc
    73921 aatcataaaa tacaacttaa cactattcca ccccatctca aaagctcttt ctgttgcatt
    73981 ttccaaatct tcttctgtaa cacctttatt tataacatct ctaagtcttt gagttcctgc
    74041 ttctggagca aaagtaagtc cagattttct tacttgttgt attttatcag caatttccat
    74101 tgaaaaatta tctaatctaa gggatggaag cgaaatccca atattattag atgcatattc
    74161 atctaccaaa taatcagtca actctgataa cttactataa tcactcgtac taagtgatga
    74221 taatgatatt tcatcatatc cagtattctt aactaattta tctaatattt ctttaagtct
    74281 tttaacactc ttttctctta ttggcctgta tatcatacct gcttgacaga atctacatcc
    74341 tctagtacat cctctaaata attctagtac tattctatca tgaacagtat ctatataagg
    74401 tactattagt ttttctggat aatctacagt ctcaacatct tttattatcc ttttagtagg
    74461 atttttagta atcccttctc tattaggagt tacactttga actgtaccat cttcattgta
    74521 cttgacatca taaaaacttg gtatgtatac tccttctatg ctagatattt tatataaaaa
    74581 atcttctcta gtcgttttat ttttcttcca ttctttatac tcatttacta cttctaaatt
    74641 aacctcttca ccttcaccta atattactac atctacaaaa tctgctaatg gttcggaatt
    74701 ataagcacaa ggaccaccaa ccattataaa tgggtcttcc aaagttcttt cttcttttaa
    74761 tattggtata ttggctaaat ctaatatatt taagatatta gtgtatgaaa gttcatattg
    74821 aagtgtaaaa gtgacaaaat caaagtttgt tataggctct cttgactcta atgcaaataa
    74881 tggtatactt ttttctctca ttatattttc catatcaact gcaggagaat acactctctc
    74941 acaaaataca tcttcatcct tatttattac atcatataat atatgactcc ctaagtgact
    75001 cataccaact tcatataaat ctggaaagca atgtgcatat cttatcaatt cactattact
    75061 agtatcttta tgaattgaat ttatttcatt tccaagatat ctagcaggct tttctacttt
    75121 ttttaagatt tttttcaagt ctactttatt catttattaa aattcctcca aacaagctct
    75181 ttatttttat tcttatttat aagtaatatt atacacgata aaatattttc tatcaaaaat
    75241 ataactaaaa tattgaattt aaaatacata ttattcacaa aatagaatag ctaaaaatat
    75301 ttaagaatat tttctattaa atttttattg aaatacaatt tttttacaaa gtatgatata
    75361 ttataaacat aaaactattg ttttacatga cttatcatga catcttttgt ttcattcacc
    75421 tcaaaatggg gtatgttact tatataatga taaattttag gagggttaaa aatgagtaaa
    75481 gtttttgaag taaaaaaaga tatctacttt actggagtag tagatgaagg tttaaaagta
    75541 ttcgacataa taatggaaac tgagtttggg acaacttaca actcttattt aatcaaggac
    75601 gaaaaaacgg ttttatttga cactgtaaag gcaaatttta aagatgagtt tttaagtaat
    75661 ttatcagagg ttactgatat agctaaaatt gactatgttg taattcatca cacagaacca
    75721 gaccatgcag gtagtttaaa atacttatta gatataaatc ctaatataga agtttattgt
    75781 actaaagctg ctaaactata cttagatggt caaataaata gaccttttaa ctgtcatgta
    75841 ataaaagatg gtgaaatttt aaatatcggt aaaagaaatc ttagatttat aactgctcca
    75901 ttcttacatt gggttgatac aatgtttact tacattgagg aggacaagac tcttttaact
    75961 tgtgatgcat ttggttgtca ctttgcaagt gttgatgcag aggtagtgaa tagtgaagat
    76021 tatctaaaat cagctaaaca ctactatgat tgtatagtta agccatttgc taaacatgtt
    76081 ttaagtgctg tagataaggt ggtaggacta aacatagaat ttgatactat attgacttct
    76141 catggtccaa tgcttactaa agacccaatg gctgctgtta aaagatatgt tgagtggtct
    76201 acagaagctg ttaatacaac taatcaaaat caagtttcta tattctactt atcagcttat
    76261 agtaatactt tagagatggc taaaaagata aaagaaggac ttgataaaga aggtgcaaag
    76321 gctgaattat atgatttaga agatatgaca ttaacagaaa tgcacgatac attagtagtg
    76381 tcaaaagtaa tattactagg ctctccaact ataaacaaaa ctatggttaa acctatgtgg
    76441 gatttattct ctgttataga cccaatggct aaccaaggca agatagcagg agtatttggt
    76501 tcatttggtt ggagtggtga aggtataact atggctgaga ctttacttaa atctatgtca
    76561 ttcaaaatgc ctgtagaatc tcttaagaaa aaattcttcc ctagtgaaga gacattaaaa
    76621 gagtgtatgg catttggtgc agaatttgca aaattagtta aatagccatt aatcacacat
    76681 aatattattt aaaataaatg attttatatt taaaaataaa ttaaaatgga gttgtctcaa
    76741 aattaatttt tctaatctaa ttttaagttc aactcctttt ttattttcta aaatgactga
    76801 tataatgata aatgcaccct ttgtgcgagg gtgcattatt tataatatgt tctatttgta
    76861 cactaatata tttgtattaa ttcacatatc tagcgtatgc taccatccct cttccagaat
    76921 tagatgccgt ttcaccaatt tccactttaa taattacatt attttcttta cacttttttg
    76981 ataattctgc tgcagaaagg ttcttaccag taaaatctaa agaaaatggc ttatcatatt
    77041 caaaaggata ctcatcaggt acaccatctg gagctaatgc aaccatacca tatggtgtat
    77101 tcataaaccc tattacagta actgtatgct ttgatgggtc taaatttgaa tttttgcttg
    77161 taaacttaat tgtatttatt gatgagttga ttaaactaca cggaactccc tcatacattt
    77221 caatttttgg acgtagatta tttccataaa cttcatcaag tattgcccct tgttgatctg
    77281 ctatcatagt gtgattccca ggtccaactg gcgcagaagg cttagctggc gtaggtttct
    77341 taacagttgg cgttggattc tgtgtcttta ctacttcgcc aacagctgga tatgctttct
    77401 tgtcaaatgg taatgggtga gtaagactct tgtatatgat gtctgctcct gctgatttgt
    77461 tggcatcaat atctacatga tacaaacgtc catcctcaaa acgaataaga ttccactgat
    77521 ggtcatttgt tgtactatgt acataataac attcaattcc catagcatca cataaagctt
    77581 tcaagctcct acacattcca gaacaagcac cttttttagt aaccaatgta taagcagtat
    77641 tggctccctc tgctgcgttc cagttaacat atgttgtgtt gtttttaatg tgattataag
    77701 caaccctaat cttagcatct gtggtcatgt tctttgtaat tttcttgttc ataaagtctg
    77761 ctgttgcttg tgctaccgaa ttaagttgag cttgtgataa ctttggacca tatatactgt
    77821 tggttccagc tacataactt ttcggtgtct ttgcagtaac atctttagct gtcataccat
    77881 aaactgttac actcatacat gaggttaaaa ccatcgttgc aatcaatagt ttagtagcta
    77941 atttcctgat ttttttcata ttagcattct cctttataat atattttact ttcaattccc
    78001 taccattaat taagttcatt ttagatttaa aatatggatt atcaacatgt atgcatagca
    78061 attcacgtgg tcatagaata tttagatttc ttggaaaatc tgttctatat ttcctatata
    78121 gttcgtatac aatccaactc acaaacctgc atttttgata tcatcacaat aaaatgataa
    78181 taattaatat attcaaaaat gttggtctgt aacaggtatg tcaattttcc aattattagt
    78241 aatggcgaga gtgaggagaa ttgtcaataa tacatttaaa attaaaattg tatacacttc
    78301 atacattctc ccctaacaac attatacaga ttataatttg tatattcaat acattttttg
    78361 gaaagtataa ttatatcaaa atatgtgatt atgtatagat acaatccact atggagaaat
    78421 ataaaagaaa agagtataac aaacaataaa aaataagatg attcaaattt tattttgaaa
    78481 catcttattt actctacaaa ttaagattat ttggtgaatt atttaattat tattttttat
    78541 taaagaaatt ttaaatctta atataaaata cgctattata cataataatc caaatacgaa
    78601 tgctactgga attaacatct ccgattcaaa ataaaagaaa tattctaaga aaatatctgg
    78661 atttactata ttcccattct tgtctaaaaa atcttttcca ttatatccta aatgctgtat
    78721 aaaattactt atgttattat aatctgcttc cctctcatac accatattag cattcaatcc
    78781 agaagaactt aaattagtgt ttccatcttt catatctatt acatatagtc taagagcttt
    78841 gactttacta tacttatctt tatcaagtat tttttcaagt tctaacttat attcaccaat
    78901 ctttgtcata tcatgtcttt ctgccaattg ctttatttct ccatcaagtt cttttcctaa
    78961 attatacata ttatgttgta aactattatt gtaataccac ataaatccag aaattaaaaa
    79021 tgcaattata cttatgtaac ctaatacttt tttaaacccc attacaattg atttatgcct
    79081 atccaatgat aaagatagtt cttttattat attacataat tcatactgca tttcatcacc
    79141 atcatcaaat ctttcaattg cttttttaca tgcttcttct tcatctaatc cttctaattt
    79201 aaattcattt gcagattcta taagatggca tctcatttcc tgttttaatt ctatagtact
    79261 tttattgtca cattttttat atagtttatc taaatattca tctattagtt tcataaatta
    79321 atccacctct cccaaaaatt gattcataac ctttttaata aatctccatt cttgtttctt
    79381 tatttctaaa aaatctttac cttcattagt taaattataa tattttcttc ttccacctga
    79441 actttgttca ttgctataat aagatttaat taaattttta gattccattc ttttaagtgc
    79501 aagatacatt gttccttctt ttaattcaaa ctcacttctt tctcttactt gctttgcaat
    79561 ttcatatcca taacaatctt tcttttctaa tatagaaaca attaatatat cgatataacc
    79621 ttttaacact tctttattta actgcattat gtaccaccgc cttttcacct actactctat
    79681 aatgtatagt acttatattt actataataa tacatagtac tctataatgc aaggtttata
    79741 ttctacaata gactcaaaaa taataaatag ctgtatatta atatttaatc tataaatact
    79801 aatatacagc tatttatcaa attttaaaaa taaatttttg ttttattaat gttgtcataa
    79861 caatatttac ttggttgaaa tagtattacc tataagttta aatatatata ataatagact
    79921 tgattctatt tcatattatt aaaatagagt aatagtttaa attctactac tctatctata
    79981 atctatttcc taattattta atctcaaatt tagctcttca tcatttaaat ctttcataag
    80041 atttctacag gtactaggtt tattgcaaca gaaagactag ggcattaaac acttatatat
    80101 ctgtttcttt agactgtaat attaatattt tttaaatacg ttgacttttg ctttttctaa
    80161 cttctctcca tgatagttct cctctatcaa gagcttttat aaatatttct gctgaagcta
    80221 gatttgtagc tattggaaca tgataaacat cacaaagtct tattaatgct tgtatatctg
    80281 tttcatgagc ctgagatgtc aatgggtctc ttaaaaaaat aactaaatct atttcttgtg
    80341 ttactattaa agaacctatt tgttggtctc cacctaaagg accagatgct aatctattta
    80401 tatttaattc agtttcatcc attattcttt ttcctgtagt tccagttgca tatagtccat
    80461 acttttttaa aattgattca taacctatac aaaatccgac catagtgttt ttcatttggt
    80521 catgtgctac taatgctata ttcatatatc taatctcctc aatttatgtt tagatgtatt
    80581 taaatttaga aatttatttt ctttcttctc atacacacat ttagtactaa tcctaagttt
    80641 gctaaagatg tcaataacga acttcctcca taacttacaa aaggtaatgt tactccagtt
    80701 actggtatta acgcaaccgt catacctata ttttggatta tctggtaacc aaacattccc
    80761 ataacaccaa ccactatgag ggttccataa aaatcttttg catctcttgc aattgctaaa
    80821 agccttaata ggaatatcat aaataagatt atcaacactg ccattcctat tacaccaagt
    80881 tcttcaccta caacagcaaa tataaaatca ctatcttgta caggtaaaaa atcttcttga
    80941 ttttgacttc cattgtaaag tcctttacca gttacgcctc cagaaccaat tgcaatcaga
    81001 gattgcataa cttgatagtt tccttttaga gttacatctt ctggatttag aaaagcttct
    81061 attcttacct tttgatggtc tgccatcatt aaatacatca gtggcattga cactaatagt
    81121 atgattattc ctctttttat caactttgag ctaagacctg ctgtgaacag cattgcaaat
    81181 atcatacaac aaaatacaat tcctgttccc aagtctggtt gtgcaatcaa taatccgata
    81241 aatggaagag aataaactac aacaggcata acttccttta acgtatttaa tttatccttt
    81301 ttggattcaa gtatctttgc ataacttagt acaaatgtta atttgactat ttcagatgtc
    81361 tgcaagtcaa gtggtcctaa gttaatccac gacctagctc caccttttac tgtcccaatt
    81421 ccaggtaata aaactatggc taacaaaata agacttatta tataaagcgc tttataatac
    81481 ctgcctagaa ggttatagtc aaaaaataat atgactataa tcattcctat accaagtaca
    81541 aaagctaatc cttgtttaat caattgatta tatgaacctg ttgaattagc atgtgttgca
    81601 ctactaagta taacaagccc aaaaataaag atagctaata ctgtaactat aagtttccag
    81661 tctaactgtt ttatcaactt aattgtcgac ttataattaa tattgatttt tttcaaagaa
    81721 tcaccgtctt tctatcttta aacatataat taaataaaag gggcatatac caataaggat
    81781 atcccccttt taagcactaa ttacatacat ctatctttta tattctttag tggtatatta
    81841 gcaactaatg cagatacagg tttgtcatca tgctcacctc tgcatttaga catttttatt
    81901 tcaagaccat catcttctat ttctgcataa ttagctatta ctttcaaaat gtctgttttt
    81961 atcatctcta aaagttgtgg tgaacaatct actctatcat gaactaaaac tagctttaac
    82021 ctctctttag caacagattt actagtttta gcctcgttag aaaaaactct aaataaatct
    82081 aacacagatg tatcctcctt tgctacttag ccatgctaaa cattttttta agtctaccaa
    82141 agaatccatc ttcaacttca agatcaagta gaggaacttc ttcatttagt atcctttttg
    82201 cgatattttt gtatgcttga ccagcaagtg acttagaatc aagtatagct ggctctcctt
    82261 tatttgttga tataattatg ctttcatcat caggaacaag acctaacaaa tctattgcta
    82321 atatttctat tatatcttgt ttatccatca tgtctccacg cttaaccata tcatttctaa
    82381 ttctatttat aactaatctt atctctttta tttcatttgc ttctaataga cctattattc
    82441 tatcagcatc tcttactgct gatatttctg gattagtaac tacaatagct ctatctgccc
    82501 cagctactgc atttttaaag ccttgctcaa tacctgctgg acaatctatt attatatact
    82561 cgaaagattc ttttagtttc tcacacaggt caatcatctg ctcaactgag acagcatttt
    82621 tatctctagt ttgtgctgct ggtaataaat atagattatc aaatcttttg tcttttatta
    82681 gagcttgctt aagcctacaa gttccttcaa ctacatctac tatgtcataa actattcgat
    82741 tttcaagacc cattactaca tctaagtttc taagtccaat atctgcatcc acaactactg
    82801 tttttttatt ttctaggctt agcgcagttc ctaagtttgc tgcggtagta gtttttccaa
    82861 ccccaccttt accagatgtt ataactataa cttcgctcat aatactaccc cctatttttc
    82921 tttctatttg tctaactttg acaaatagct ttcgattaca attcttccat tacttacaaa
    82981 ggctatttct gggctaattt ctgattcact ttcataattt tcttcatcat ctggagcttc
    83041 tgcaatatta tctgctattt gtaatatttt aggattaaga tttccagcaa caacatatgc
    83101 aaattcattg ccttctgcac ctgcatgtac aaaaccttct attttaccca ttacaaaaac
    83161 attacctcct gctacaacct tagcaccagc atttatatct cccataacaa ctgtatttga
    83221 ttttgaaagg acttttgaac caggtttcat atcattcaat ataactacat caccttcaaa
    83281 ttcaatattt tcaccagatc ttagggtatt gacatatttt gttggaaaat tactattttc
    83341 ttcaattttt tgatcttcaa caaattccac atcaaatcta gaagtaattc cttctttaag
    83401 ttctagaatc tccatgtcag ttaaacaatc actattaatt ttactaattt tagccccatt
    83461 aaaaaagcca acatatgctt ccaatttgtt tattattttt tcctgtattt cctcaaatgg
    83521 agcttctctt tttatattta ctattatccc tcttttatta cccttgaact ctactagttc
    83581 ttgactacaa atttctctta aagacataac aacctccaga gtgatatatc atttatattc
    83641 ttcaaatacg ctttaaaccc ttttattttt tctatataaa gctaaagttt atacatagat
    83701 tgagctatct attaatttgt gactcgaaat ttatattttc gtcagaagaa ttgttaacat
    83761 cattcgtctt gttgtcattt ttactattag ttggctctaa tcccatataa gttccaataa
    83821 cttctctagt tggtaaaagt gagaatacac ttgaatcacc ttgaggaatt acacatacaa
    83881 cagctatttc tgggtcatct gctggagcaa aagcaacagt ccacgtaaaa gaaccataat
    83941 cttgtttaaa tctatctatt tggtcatctg ttatttttgg atttaattct tttatagctt
    84001 ttcttagata tgaagaattc accttatcag tgtcctctaa tctaactttt actccatctt
    84061 ctaattcttc ctctaatttc tttctttctt cgtctttcaa atctttattt tctaatttct
    84121 tttttatttc cttttcctta gctaatgaaa gctctttttc tttttctgct ttcatcttat
    84181 cagctagttt aatcgcatca tttaggttga cattataaga tgacatatga ctttttaaat
    84241 attcatactc gttatctgta ggtattttcc cacttttctc agctgtacca gtttttgctg
    84301 ctacatctat tggaaaatca gcaaaagcac tcttcgctgt accttgtctt gcaaccaatt
    84361 tcataccttt tgttaattct tttaagttat ctggattttt aaaaggtatt ttttcgactt
    84421 tcttctgatc attaatttta actgatgaat aatcacttga aacagctcta tcaactacag
    84481 ataattctac taaattacct ccatttgcta tagcagccac atatcttgat atctgagctg
    84541 gtgtataggc attctcacct tgtcctatag ctaagttgaa tgtatctgca gtactccatt
    84601 ttgcaaagtt aaaataactg aatactgcta aatctgctac ttcttcaatt ctatcttctt
    84661 taacatccaa tttttttagc ctattcattg tttcaactct accaggagtt ttcttttctg
    84721 ctgcccagct tacaatctca ttgattcttt tctcatattc tttaggattt ttttctctag
    84781 ttatatcaac aaaatcattt gccatttctc tttctaaatt tgactttaga agagcttgtg
    84841 ttgatttcaa tttatcttct gtgctaggaa ctttacctgc tctttcttct acttcatctt
    84901 gaagccctgt attttggtca agcccaaata attttgcata ctcaagtatt ttatttgcat
    84961 tcatatctat accaatgctt tttccatctg gccatgtttt acctgtacca attgttgcca
    85021 tatatatatt acaagactct tgtattgctt tatataagtt tgtcatacca tggtttcctc
    85081 taccatggtt ccatacatag tcaccaaaag attttttacc taacattata accccagggt
    85141 catttatagt aaagtttgga tccagaccat tttctaacgc tgccattgaa gttaccatct
    85201 taaatgaaga acctggctga aattcccctt gtgtaaccaa gtttaaaagt ggacttcctg
    85261 ctagtaagtc atttggattt tttggttgaa gtgctttata atcctctgta gaaatacctt
    85321 ttgcaaattt atttgggtca taattaggat aacttgatga agctaatact tctccatttt
    85381 taacatcaat agctataaga gctgctgact gtgcttttcc tgcatatgca ctaattggtt
    85441 tgtccccaaa tttactttta aaagtacctc ctttagaagc aacttcgatt atctgtttta
    85501 atgcattatc tgaaacttct tgtaaatctt tatctagtgt aagatacaca gtatctcctg
    85561 atttaggttt tttagattca atttctttac tgattctacc caatgcatca actttaacca
    85621 ttttataacc atcagttcct cttaatctac tttcattact cttttctact cctgcaagac
    85681 ctaccatatc tccagtttca taaccttttt gaaggtatga ttctatttga gtggatggca
    85741 tttttcctac atatcctaac atatgagaag ctaatgaccc attaggataa tatcttactg
    85801 gttctactgc tattgaaaca ccaacaaggt ccatagcact ttcttcaatt tgagcaatcg
    85861 tagtttctcc tacatcctta gcaatagtta ctggattata ttgagaatat ccttttgatt
    85921 ttatcaaatc ccttacgacc attatttttc ttgcatcttc atcactcaaa cttttatcaa
    85981 tttctaatgc atcactattt cttacttttt caaacgcttc tttggcactc agtttagttt
    86041 cttttatctt ataactagcc aaccaatctc tcttatctct ttctgctgtg aacatccatt
    86101 tactaactaa aataggtgga taatatccat tttcatttag ttttgcctgt aatcttgtcg
    86161 catccaatct tttatcttct tgagataata ttccagctga tataagtttg tctaccaagt
    86221 aataaaaact ttccttagca ttataatcat ttggaatacc attttctgat ttatattccc
    86281 taatatctct gtcatatgta taatagtact ttccattttc tacatatata ggaaactcat
    86341 ctacatattc ttcaccattt ctctccagca aatttataag ttttaatgat atttcatttg
    86401 ctcttgaatg cttatttgca tctttcttat taatatcgtt acctgaaact tgaacagtaa
    86461 ataagttttt atttcctgcc aacattcttc catatctatc ttttatttct cctcttggag
    86521 cttctattgc caacttttta tatgttttat tctcagctaa ctcattgtaa tattcatatt
    86581 taaatgtagt catatagatt atttttacta aaatagctaa aaatatcacg cccatgacta
    86641 tcttaattat ttttagtcta tcaacttgtt tattttcttc catgaaatca ccaactaatc
    86701 ttctttaagt tttaaaactg ctcttttaga taagcgatat attactaaac caattaaact
    86761 attcactatt ggtgctacta gtagaccttt tacaaaaagt gttgatatgt tgtagctttg
    86821 ataaattata ctagatgtac ctatattgac aattgaatca attaaactgc ctataaaaat
    86881 taaaacgaaa gtaataatat aactaccttt atatagctga tctctcatgt atccaattgc
    86941 atatgcaata ataaacaaaa ttaatgcatt tacaccaaaa attccaccaa cggttatgtc
    87001 tttaataagc cctattattg ctgctataat cccaccttct aattcatcta aataaagaga
    87061 gattatggtt acatatataa gaactaaatt aaagcttatg ccaaaaatat ctatataatt
    87121 tgttatacta ttttctatag tcataagcac aatccccaac agacaaagta aaactttttt
    87181 cattgttttt atccccttta tcctatattt cttggttcta tgactacaac gtcatttata
    87241 ttcttaaaat caacataagg ttttacaact acatatttta atgacttatc tttatcatca
    87301 accacttttt caacttcgcc aattggtata ccatctggga agaatccaag acctgatgtt
    87361 gttattatat ctccttgaat tacattatat gatgagtcaa acatatatcc ttgcaaatat
    87421 cctttactat tataactatc tttattatct aaagtagtat tttgagtaat tgtaccttta
    87481 gcattggcat cctttgctag tttaaaactt acagatgcct tagaatccaa tagtgaaatt
    87541 gccttactat aattgttgga aacttcataa actatgccaa ctaatccact accattcata
    87601 actatacttt ctttttttac acctgaattt tttcctgcac ctatgacaaa actctcatac
    87661 cagtttccat catttttacc aacaacactt gctgaaatac ttgtcgcctt atactcttct
    87721 tgtacaaagt tcagtgtctt ttttaactct tctagcgatt ctgttttatc tagtttggta
    87781 tttaaagcta tcatattttt tttaagtttt tcattttctt cttctaattt ttttaccttt
    87841 ttagaattat ctttaaaatt tgctacatct ttaaaaccat ttttgataaa agaaaatcca
    87901 tcatttattc ccttacctac acttgtaata gcatctgaag ctactcctaa atttactgga
    87961 ctaccactag aaaatttacc aattgatata cccacaattc caattaaagt gatagcaact
    88021 acgcccgttg ctatcacttt aacattaatc ctttttttct catttttgtt cttgtcaaat
    88081 ctcaaggcca tcaccaactt ctaattctta gtattcatca ttaatacttt ttcaaagatt
    88141 tcttggtctt caacagattt acctgttcca agtgcaacac aatcaagagg tgtttctgct
    88201 atttgtacag acataccagt ttcctgtgta ataagcttat caagacctct aagcaaagcg
    88261 cctcctcctg ttagcattat tccgttttcc attatatctg aggctaattc tggtggagtt
    88321 ctttctagtg tagattttat tgcatctact attgagctta caggttcttt taatgcttct
    88381 ctaacttctg tagagcatat ttctattgtt ttaggtaatc ctgagattaa atctctacct
    88441 cttatttgca tatttatttc ttcatcatct ttaaatgtag aacctatgtt aatttttaca
    88501 ttttcagcag ttctttcacc tatcataaga ttatattctt tttttacata agccactata
    88561 gactcgtcaa attcgtctcc acctattctt atagatttag cagtaacgat tcctcctaaa
    88621 gatattacag ctatttctga agtacctcca ccaatatcaa ctaccatatt accttctggt
    88681 tcttcaactt ttaatccagc ccctatagcc gctgccattg gctcttctat aaggaatgca
    88741 tctttagctc ctgcttgtct tgcagcctct tctatagctc tcttttctac ttctgtaact
    88801 ccaaaaggaa cacatatagc tattcttgga cttacaacac cttttttatc agccgctttt
    88861 tgtataaaat agctaatcat tgattgagtt atgtcaaaat cagctattac tccatctttc
    88921 ataggtctta tagctactat atttcctggt gttctaccta tcattttttt ggcttcttca
    88981 cctactgcta atacctcttt actatcatct cttattgcga caactgatgg ttctctaaca
    89041 acaatacctt gtccttttat gtaaactaat gtatttgcag tccctaaatc tattcccata
    89101 tcctttgtca tcttattaaa actaaaaaaa cctttttttt ctttcttttt ttccttagcc
    89161 atgattttac tccttcctac tattaagaat catgtttaaa acattctcct attattttat
    89221 cacatataaa gtcaaattat catattttct ttaaagctaa agtataatcc atctccaata
    89281 attatatggt ctattatttc tattccaatt atttctccac atttaataag tcttgatgtg
    89341 atatttttat cttctcttga tggttctaca cttcctgaag ggtgattatg catcactatt
    89401 atcttacttg cacttcttct tatagcttct ttaaaaacct ctcttggatg gactaatgac
    89461 gagcttaaag tccctataga tacatctaca tcagatatta tctcattttt tgtgtttaaa
    89521 agaaccgctt taaaaacctc tttttttaaa taccttaggc tatccatata atacctctgt
    89581 atatcccaag gattccttat tttatacttt ataggtttaa aactagctac tctagagcct
    89641 agttctaaag ctgctttaat ttgggttgat tttgataatc caattccatc tatattacac
    89701 agctcctcta ttgtcatatc ttctaaatgt ctaataccct gaatatctct atttattata
    89761 tagttggcta attcaatagc atttttattc ttgttgccag ttcttaaaag tatagccaac
    89821 aactcagcat ttgataagct ttttacgcct ttggcaagca tcttttctct gggtctttcc
    89881 tctggtgcca tttcttttac cttaatggtt gaattaaatg actttttcag ataaatcaca
    89941 ctccataaaa aagatttata ctaaaatatt tttttaatag gtcgcctaac cttgaaattg
    90001 gaagaccaac tatattaaaa taatctcctc taatttcttc aactaacaaa gctccataac
    90061 cctgtatacc ataggctcct gctttgtcaa atgactcatt agttttcaga taatcctcaa
    90121 tatcctgctc tgataacgtt ttaaatttca cattgctgat tacatagtca attacctttt
    90181 tatcttcgca caagtttatt aagctaatac ctgttatgac ttgatgctct cttccagata
    90241 gtctttttaa catatttctt gcacaagact catcttttgg ttttcctaaa atagcattgt
    90301 ctaaaacaac aactgtatca gctccaataa caacatcttc attatgttca gatgctacag
    90361 acatactttt ttcaaaagct aatctcataa ctaaatgttt tggtgtttca ccttccaata
    90421 taatctcatc aatttcattt ttaattatat caaatcttac attcgtattc tctagaattt
    90481 ctttccttct tggagaggct gatgccaaga ttatattcat ttattcacct catttgtacg
    90541 taatttgtac ctatttagtc tatcactata ttgtaaaaaa ttcaacagaa atgtcgaacc
    90601 ttttttgtta cactattgtt atatttatta ccattagaat aaatatacat aaaatagagg
    90661 tatggaaatt attccatacc tctgttttat gtactcatta gagatatgga aattattcca
    90721 tatccttatt ttgtccatat tacaaaattt taatagcttt ctttggacat ttttccatac
    90781 ataaatggca tcctacacat ttatctgcat ctaccttatg tttttctttt aattctccct
    90841 ctattgcatc aaacttacat tgttttttgc acactgtaca tccaacacaa agttcttggt
    90901 caatagtaac tttctttttc ttagttatgt cacctgatat tacttttgta gggcattttg
    90961 ctacacattg catacaacct acacacttat tagggtctat cttagctatt ttgtcttcaa
    91021 atattattgc atcaaattta caagctttta cacacattcc acagccaata catccagcag
    91081 tacatttttc cttaacagct ttaccaaact ctttgctatt acactctaca actacttctt
    91141 gactttctgg ttttgtgatt attataccct taggacaaac ttctttacat tttccacaat
    91201 ttacacactt ttcttcatct attactgcta ttccatcaac tatagaaata gcatcaaatt
    91261 tacaagcatc tttacatgtt ccaagaccta aacatccaaa tttacacatc ttagcacctg
    91321 agtttaaaac atttgctgct ctacaatctg atattccttc atactcatat ttatcttttg
    91381 cacttgaaca agttccttta caaataactt ttgctacttg tttctcacct gcttcagcac
    91441 ttattcccat tatttctcca acttttgcac caacatctgc tccacctaca gggcaaccat
    91501 taactggtgc attgccttca actatcgcat ttgcaagacc tccacaacct ggaaatccac
    91561 aacctccaca gttggctcct ggaagtactc ctagtatagc ttccactctc tcgtctactt
    91621 ctacagcaaa ctttttagat gcaaaatcta gtactatccc aaagattaag cccataattc
    91681 ctaaaactaa tacagctgta agtatcacca ttttcacccc tcttttttat agctttataa
    91741 gtcctgtaaa tcctaagaaa gctattgaca ttaaacctgc tgatataagc gttataggga
    91801 aacctttaaa tgcttctggt atatcagcta attctagtct ttctcttata cctgcgaata
    91861 ttactattgc taaagtaaat ccagctccgg ctccaaatcc gtttattatt gtttctacta
    91921 aattataact tttttgaaca ttaactagag ctataccaag aactgcacag tttgtagtta
    91981 taagaggtaa ataaactcct aatgcttgat ataaagatgg actcatcttt tgaattacca
    92041 tttcaacgaa ctgaactata gaagctataa ccaatataaa agctattgtt tgtaagaatc
    92101 cagttccagt ttttactaat aaaatctgta taaaataagt tataattgaa gctagagtta
    92161 aaacgaacgt aactgcaact cccatcccta ctgctgtatc aaccttctta gaaactccca
    92221 agaaaggaca tatacccaag aattgagatg ttataacgtt attaacaaga acgatactta
    92281 aaaataaaag tattaaattc atttattaca cctccactat gcttttttac ttcttaattt
    92341 attaaatcct gcaaataaga atcctaatgt taagaatgca cctggtggta atataaatat
    92401 taatactggt tggaaagatg ctccaaataa tgtcattcca aataaactac catttccaag
    92461 taattctctt actgcaccta ttattgtaag tgcaacagta aatccaagac cttgacctac
    92521 accatcaact attgaaggca tagctcctgt tttgaaagca aaactttctg cacgagctaa
    92581 tattaaacag ttaacaacta ttaatggtat atatatacca agagccttat caagagcagg
    92641 aacatatgct tttaaaagca tacctactat tgtaacaaat gtagctataa ctactataaa
    92701 tataggtatt cttatcttat ctggtgtaat ttttcttatt aaagatataa ctaaatttgc
    92761 acatattaaa actgctgctg ttgaaagacc cataccaatt ccatttattg ctgaagttgt
    92821 aacggctagt gttggacaca tacctataac ttgaacaaat gttgggttct catctattag
    92881 cccattttta aatactttag ctaaattcat aattaaccct ccttatttat taagagcact
    92941 attgaaaaca tctatagcag catttactcc tgtagtaact gctgtagatg taatagttgc
    93001 acctgatatt gctaatattt gattatctgc tgttgtactt ccttttgcta cttcaagagg
    93061 ctcagcagtt tttcctttgt attgaccctt aaatgcatca tccttagctt ttgctccaag
    93121 acctgctgtt tctgacatat taccaatgtc aacacctgta acttgacctt cagatgatat
    93181 acctaccata acttcaattt ctccaccata tccacttggt ttagctttta aagtatatcc
    93241 aaccacttca gaaccatcta aaccttcata cacttcagct ataagatcat ctccaaggcc
    93301 tttgtaagta tcttccttca tttctttaaa ttcttttgct tctggaagga ctgcttttct
    93361 aagttcatta tttgcttgta tatttctttg ttctattact ggagctgtaa cctgattagt
    93421 agctccaaga gctaatgcag ctattgcact tatagcaagt aaagtaccac ctaatctcac
    93481 catactgttc attatttagc cacctcccca aatactctat ttctaacaaa cttatctata
    93541 agtggagcaa gacagttcac taatagtatt gagtaagata caccttctgg atatccacca
    93601 tacattctta taacagttgt tataagacca gctaatacag cgtatattat ttgacctttt
    93661 ttagtcatag gcgaagttgt ataatccgta agcatgaaga atccacctaa cataagtcct
    93721 ccagcaaaca gttgatatat tgcgaaagtt atattaaatc cacctaaaag gaatgttaat
    93781 ataaatacag ttgctatgta aaatactggt ataacataat ttattatgcc tttatacatt
    93841 aggtatactc cacctaataa taataatatt gctgatgtct cacctataca tccaccaata
    93901 ttccctatca tcatgtcagc aagactaatt ccattgctag aaagatctgc cagtcctgct
    93961 gctccatttt tcaaaaagct taatggagtt gctgtagtaa ctgcatctaa atttttacct
    94021 atccagttaa cacctggttg agtccaagca gtcattgcta ctgggaatga tgctaataaa
    94081 aatgctcttg cagcaagtgc tggattgaca aagttacatc caattccacc aaataccatt
    94141 ttaactacta atattgcaaa tattcctcct actagacaca tccaccaagg aagtgatgct
    94201 ggtacgttaa atgctaataa taatcctgtt acaacagctg aaaaatctcc tatagtactt
    94261 ttttgcttcg taaatttttg atataaaaat tcagtaccta ctgtaccaag tatacaaaaa
    94321 aacatagcac tcaaagcatt aagtctaaag aagtatactc ctgctactgc tgctggaagg
    94381 agtgctataa taacttgttt cattatatat gaagtatctt cattacttct cacatgagga
    94441 gaagatgata ctatcaactt attttccatc gtttttcccc cttaactttc tttatttctg
    94501 agcagcttgc tttcttctct ttgctcctat ttctctcttt gctgatctta tagattgaag
    94561 taatggtctt cttgctggac aaataaatga gcaagaccca cattctatac aatccattgc
    94621 tctatgatat tcagctgtgt catagtcatc ttttaaagaa tatgcactta tgaaaagtgg
    94681 ttgtaagaat gatggacata catctgtaca tcttccacat cttaaacagt tttgaatatc
    94741 tggagtacgt gattcttcct catccaaaca taaaattcct gaagaacctt tgttagtcgc
    94801 tatttcagta gtatattgag ctattcccat cataggtcca cccattataa ccttaccaat
    94861 cttcttatct tctttaaatc caccacattg ttctattatt tctgaaacta atgttccaac
    94921 ttttgttatt aggttctttg gctctttgat gcaactacct gttattgtag tgattctttc
    94981 aaccaaaggc atacctgttt taatagactt agctatctgt gctgctgtgg caacgttgtc
    95041 aacaactgca cctgctgcta ttggtaatcc accagatgga acttctttgc ctgtacacgc
    95101 ataaataagt tgtttctctg ctccttgtgg atatttcact tgaaggccaa caacttcgat
    95161 ttcgctatag tctttagcta cattttgaat tgcttctata gcatctggtt tattcgtttc
    95221 aattcctata aatccttttt tgacatctaa taccttcatt aatgctctta aaccaaacac
    95281 aacatcttct ggattttcaa ccattaatct gtggtctgca gttaagtatg gttcacattc
    95341 tgcaccattt aatataacta cttctgcctt gctatctggt ggaggagata cttttacatg
    95401 gttagggaat gtagctccac ccatacctac tattcctgcc tcttttatga tacctactat
    95461 atcttctttg ctcaaatcct ctaactgacc ttttggttga acactctcat gtaattcttc
    95521 cttaaagtca ttttcgataa ctacacattg agctgaacct cctggtactt catgttgttc
    95581 aatagcaata actttacctg atactgaaga atgaacgtta catgaaacaa aaccttgttg
    95641 ttcacctatt ttttgcccaa actttacttc atcaccaact tcaacaatag gtttagctgg
    95701 tgcccctata tgttgctgaa gaggaatgta aactatcttt ggagcttgag ctttttcaag
    95761 tgcctttgtg ttagagtact cttttctata tggaggatgt atccctccct taaaagttaa
    95821 gagtttcatt gttttacccc cttatctata aaaaaacatt ttttctgcct tctataagta
    95881 tactatatat attaaaaaat cttaacaatt gttaaaaaaa tatccgtatt tttataattt
    95941 attttataaa tcaatacctc tatattaagt caaaccgagg aaaaggattg cttaattaaa
    96001 caatcctttt catatagctt tctaaagtcc aaattttctg tattttattt tatagaattt
    96061 ataaacatag agtactcctg catggatgat aaaagcagac ttttacttac actatatttt
    96121 tcttcatttg ctgcttttga agctgtatca attttttcat tcacattctt tacatactct
    96181 attaaattat ctttaaataa acgcatttca gaatagtcaa tcttttcagc ttcttttgat
    96241 atattttgtg atattgcctt tctatttgtc aaaatagtat tatatttttc catatctaca
    96301 ttattttcag cattcgacca aaaacttgat tcttctttaa agtttgttat taatttattt
    96361 aattctttag atatactctc tatataagcg tagttacttg tatactctaa agacaacata
    96421 ggtgcatcta agtgtgataa aaatgcatct ggaaacacct ttcttacact gcttatctgc
    96481 tttctaacat cattttcatc aaaagaggaa taggtctgta ttttttttac tccatccttc
    96541 tccataactg aaaaaggtat gtcattatta actaatgatg tctgtatttt tttcaaatca
    96601 tcattattat ctattgcagc aacttgaata gtatacatat cccagccttc tatagtagct
    96661 aatttgactt cttctggctc tttagtgtta tttaccgctt tttctggatt agtttcttgt
    96721 ttatcatctg aattggtttg tgatttagat tttttccctt tttttattga atttaattca
    96781 tcagatatat ctttataagt tatatctgat gaattattta gaaatggtat tttagccgat
    96841 acatactcca atatcttgct atcttttatt tttgtataag aataatagcc aattagacac
    96901 aagcaagcaa tcgttataac atatttacgt aaatcaatct tatgtctatt attattaaat
    96961 cctctgtata tcgtttttct tctattcatt agtttgtccc tcccaaaatt atattattaa
    97021 taaagtatat ttttaagagt cacaatttat gaatataaat aattatttct tctgattttt
    97081 taataagaaa ttgtacttac taaacaaatc ttcttgcacc aagtaatact ttagaccaat
    97141 atgaactagt taaattatcc actttaacct tattaggatt agtctgagta ccactacaat
    97201 ggataaattt actatttcct acatatatac ctacatgatt agttgttcca tcaccatcag
    97261 tgtcaaaata aactaaatct cctggtgcat aatttcccat agaaattgca cttccaaaat
    97321 tagcctgttc tctagaaact ctaggaatag aaacgcctac agaatgttta aaaacatact
    97381 gtgtaaatcc agaacagtca aatttatcag gtcctgaagc tccccattga tatggtattc
    97441 caataagagt atatgcaaaa tctacgattg ttgaaccttt tgctgtagat ttatctgaat
    97501 ttccagtaaa tgatggcttt gagttactat tagattgaga attttggtta gatgatgaat
    97561 ttggtttttc ttttgtatcc tcattagtct cagaaatata actggcactc acccatccaa
    97621 ttgttccact cgataacttt accttatacc aaccattact tttagctttt aattcaacaa
    97681 catctccacc atttaatttt cctattatag aatagcttgt cccaggacca cttcttacat
    97741 ttaaactcac agcagatgtt accttaccat tcttgccttc aattgattct ttgctagatg
    97801 gttttttatc agaattatta gtactatttg aattactaga attgttcgtt ccaacatctt
    97861 ctgatgtttt agaaatatat tggctactag cccatccagt aactccattt gatagtttta
    97921 ttttatacca tccattgctt tgttctagta actttactac atctccatta tttgctttac
    97981 caactaatga atagttagta cctgcaccac ttcttacatt cagtctagaa ctcacattta
    98041 ctttaccatt tccactaata gttgtcccac ttgaagttgg attattttga cttgatgtac
    98101 cttcagacga accacttgtt gatatgtatt ttgcacttcc ccatccaaca acagaactcg
    98161 atacccttac tttataccat ccattagact tttctaaaat ctctacagtt tttcccttgt
    98221 ataattttgc taccttatcg caatcagaac ttggtccact tcttatattc aaggcacttg
    98281 ctgttactgt tcccttttca agagcacttg catttatgga gctaacagat actgctactg
    98341 ccccaattcc taaagctgct atagcttttt tcactactat cactcccccc aacaaatttt
    98401 tatagtcata aattcataac attattatcc atttttagtc cctacaattt aatgataact
    98461 tattttctta ttaattgtaa agaaattata aacatatcct taaaattagt gatttttagg
    98521 aagtatttca atccaataat tatctgggtc cgctataaaa taaattccca tactttcatt
    98581 ttcaaaacat atgcaatcta aatctttatg aagttttttt gcctcatcaa actcctcaac
    98641 ttctaaagcc agatgaaatt cattgtctcc taaattatat ggtctatccc aatctctaag
    98701 ccaagttaat tctaatgtat gactagtaat gccatctcct aaataaacta atataaaact
    98761 tccatcttct gcttccttgc gctttacctc tttaagccct aaagcttttt tataaaaatc
    98821 taaacttttt tctaaattag taacattgaa attgttatga caaaaattaa acttcatttt
    98881 gaaatcactc tccaattttt atttttttta tattatatca ttattataaa tttatttaaa
    98941 tcatattaag ttaaaaattt gaatgatgat gaccaattac caaatcatgg taaattattt
    99001 aaaagaatat gtcaaaaaaa gaataagctc tttcaaatgc ataaaaaatt tatacccaag
    99061 ttatacttaa tcatgcaaat tggcagaact tattctttaa aactctaact tttaagtttt
    99121 tctttaataa ta
//
LOCUS       DAEMHZ010000002        87578 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000002 DAEMHZ010000000
VERSION     DAEMHZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 87578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.002"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(243..1004)
                     /locus_tag="KPK72_000101"
     CDS             complement(243..1004)
                     /locus_tag="KPK72_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2487358.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1223..1699
                     /locus_tag="KPK72_000102"
     CDS             1223..1699
                     /locus_tag="KPK72_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2487359.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEIDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1797..1949
                     /locus_tag="KPK72_000103"
     CDS             1797..1949
                     /locus_tag="KPK72_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG2487360.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(2109..2297)
                     /locus_tag="KPK72_000104"
     CDS             complement(2109..2297)
                     /locus_tag="KPK72_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG2487361.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2658..3008
                     /locus_tag="KPK72_000105"
     CDS             2658..3008
                     /locus_tag="KPK72_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2487362.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            3027..4670
                     /locus_tag="KPK72_000106"
     CDS             3027..4670
                     /locus_tag="KPK72_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG2487363.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4751..6808
                     /gene="recG"
                     /locus_tag="KPK72_000107"
     CDS             4751..6808
                     /gene="recG"
                     /locus_tag="KPK72_000107"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG2487364.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISVLPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6867..7421
                     /gene="rsmD"
                     /locus_tag="KPK72_000108"
     CDS             6867..7421
                     /gene="rsmD"
                     /locus_tag="KPK72_000108"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG2487365.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7423..7920
                     /gene="coaD"
                     /locus_tag="KPK72_000109"
     CDS             7423..7920
                     /gene="coaD"
                     /locus_tag="KPK72_000109"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG2487366.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7924..8448
                     /locus_tag="KPK72_000110"
     CDS             7924..8448
                     /locus_tag="KPK72_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487367.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8792..10711
                     /locus_tag="KPK72_000111"
     CDS             8792..10711
                     /locus_tag="KPK72_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2487368.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            10724..11011
                     /locus_tag="KPK72_000112"
     CDS             10724..11011
                     /locus_tag="KPK72_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2487369.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11144..12403
                     /locus_tag="KPK72_000113"
     CDS             11144..12403
                     /locus_tag="KPK72_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG2487370.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINRAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFVGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            12406..13146
                     /locus_tag="KPK72_000114"
     CDS             12406..13146
                     /locus_tag="KPK72_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2487371.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVGPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            13487..14779
                     /locus_tag="KPK72_000115"
     CDS             13487..14779
                     /locus_tag="KPK72_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2487372.1"
                     /translation="MEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSKKDGEKE
                     KLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDV
                     GYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLY
                     YNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNW
                     YPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEMIESVFG
                     PGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKEL
                     AYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPLVVGPHG
                     VEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            14877..15794
                     /locus_tag="KPK72_000116"
     CDS             14877..15794
                     /locus_tag="KPK72_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2487373.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            15810..16619
                     /locus_tag="KPK72_000117"
     CDS             15810..16619
                     /locus_tag="KPK72_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2487374.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            16677..18425
                     /locus_tag="KPK72_000118"
     CDS             16677..18425
                     /locus_tag="KPK72_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG2487375.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDMLVEQYLPQTFWVEGQVPEDFEENNFNIGIDIFKSFGYEDEEKACTIDVP
                     VEVKNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIIDNYLKELASLGEKV
                     ATIIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            18687..19709
                     /locus_tag="KPK72_000119"
     CDS             18687..19709
                     /locus_tag="KPK72_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG2487376.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSNNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            19714..22020
                     /locus_tag="KPK72_000120"
     CDS             19714..22020
                     /locus_tag="KPK72_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2487377.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFTLICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            21992..23107
                     /locus_tag="KPK72_000121"
     CDS             21992..23107
                     /locus_tag="KPK72_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487378.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            23125..24543
                     /locus_tag="KPK72_000122"
     CDS             23125..24543
                     /locus_tag="KPK72_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG2487379.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            24558..25766
                     /locus_tag="KPK72_000123"
                     /pseudo
     CDS             24558..25766
                     /locus_tag="KPK72_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25847..27235)
                     /locus_tag="KPK72_000124"
     CDS             complement(25847..27235)
                     /locus_tag="KPK72_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG2487380.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            27576..28910
                     /locus_tag="KPK72_000125"
     CDS             27576..28910
                     /locus_tag="KPK72_000125"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG2487381.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(29039..29956)
                     /locus_tag="KPK72_000126"
     CDS             complement(29039..29956)
                     /locus_tag="KPK72_000126"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG2487382.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDV
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            30164..31048
                     /locus_tag="KPK72_000127"
     CDS             30164..31048
                     /locus_tag="KPK72_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG2487383.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            31261..33102
                     /locus_tag="KPK72_000128"
     CDS             31261..33102
                     /locus_tag="KPK72_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG2487384.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGEHTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVLPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELINKNHESDLILSENEKNINDNEDNLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            33407..34114
                     /locus_tag="KPK72_000129"
     CDS             33407..34114
                     /locus_tag="KPK72_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2487385.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            34108..35418
                     /locus_tag="KPK72_000130"
     CDS             34108..35418
                     /locus_tag="KPK72_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2487386.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTIVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            35535..36218
                     /locus_tag="KPK72_000131"
     CDS             35535..36218
                     /locus_tag="KPK72_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487387.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            36211..38598
                     /locus_tag="KPK72_000132"
     CDS             36211..38598
                     /locus_tag="KPK72_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2487388.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            38878..40020
                     /locus_tag="KPK72_000133"
     CDS             38878..40020
                     /locus_tag="KPK72_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2487389.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            40039..40563
                     /locus_tag="KPK72_000134"
     CDS             40039..40563
                     /locus_tag="KPK72_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2487390.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            40663..41481
                     /locus_tag="KPK72_000135"
     CDS             40663..41481
                     /locus_tag="KPK72_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2487391.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            41559..41870
                     /locus_tag="KPK72_000136"
     CDS             41559..41870
                     /locus_tag="KPK72_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG2487392.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(42047..42730)
                     /locus_tag="KPK72_000137"
     CDS             complement(42047..42730)
                     /locus_tag="KPK72_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487393.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(42823..43464)
                     /locus_tag="KPK72_000138"
     CDS             complement(42823..43464)
                     /locus_tag="KPK72_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2487394.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(43638..45641)
                     /locus_tag="KPK72_000139"
     CDS             complement(43638..45641)
                     /locus_tag="KPK72_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG2487395.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMEHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAMSLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLSSFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKVPIASRTAIVLLDNGI"
     gene            complement(45794..46432)
                     /gene="recX"
                     /locus_tag="KPK72_000140"
     CDS             complement(45794..46432)
                     /gene="recX"
                     /locus_tag="KPK72_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG2487396.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            46708..47397
                     /locus_tag="KPK72_000141"
     CDS             46708..47397
                     /locus_tag="KPK72_000141"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG2487397.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            47398..47970
                     /gene="yqeK"
                     /locus_tag="KPK72_000142"
     CDS             47398..47970
                     /gene="yqeK"
                     /locus_tag="KPK72_000142"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG2487398.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            48087..48437
                     /gene="rsfS"
                     /locus_tag="KPK72_000143"
     CDS             48087..48437
                     /gene="rsfS"
                     /locus_tag="KPK72_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG2487399.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            48791..51211
                     /locus_tag="KPK72_000144"
     CDS             48791..51211
                     /locus_tag="KPK72_000144"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG2487400.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDINWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            51800..51967
                     /locus_tag="KPK72_000145"
     CDS             51800..51967
                     /locus_tag="KPK72_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487401.1"
                     /translation="MWRFLIRIYSQGMVASGDMERNQFIDMVTIFLSNLMKESQEYHT
                     EVRQELTGGIN"
     gene            52066..52734
                     /locus_tag="KPK72_000146"
     CDS             52066..52734
                     /locus_tag="KPK72_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2487402.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(53041..53313)
                     /locus_tag="KPK72_000147"
     CDS             complement(53041..53313)
                     /locus_tag="KPK72_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2487403.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(53537..53958)
                     /locus_tag="KPK72_000148"
                     /pseudo
     CDS             complement(53537..53958)
                     /locus_tag="KPK72_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(53977..54075)
                     /locus_tag="KPK72_000149"
                     /pseudo
     CDS             complement(53977..54075)
                     /locus_tag="KPK72_000149"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            54358..55728
                     /locus_tag="KPK72_000150"
     CDS             54358..55728
                     /locus_tag="KPK72_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG2487404.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            56087..56245
                     /locus_tag="KPK72_000151"
     CDS             56087..56245
                     /locus_tag="KPK72_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487405.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     regulatory      complement(56766..56863)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            57089..57472
                     /locus_tag="KPK72_000152"
                     /pseudo
     CDS             57089..57472
                     /locus_tag="KPK72_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(57661..58656)
                     /locus_tag="KPK72_000153"
     CDS             complement(57661..58656)
                     /locus_tag="KPK72_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG2487406.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(58777..60405)
                     /gene="aspD"
                     /locus_tag="KPK72_000154"
     CDS             complement(58777..60405)
                     /gene="aspD"
                     /locus_tag="KPK72_000154"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG2487407.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            61064..62281
                     /locus_tag="KPK72_000155"
     CDS             61064..62281
                     /locus_tag="KPK72_000155"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG2487408.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            62376..62756
                     /locus_tag="KPK72_000156"
     CDS             62376..62756
                     /locus_tag="KPK72_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2487409.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            63040..63522
                     /locus_tag="KPK72_000157"
     CDS             63040..63522
                     /locus_tag="KPK72_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2487410.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            63543..64358
                     /locus_tag="KPK72_000158"
     CDS             63543..64358
                     /locus_tag="KPK72_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2487411.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            64408..65958
                     /locus_tag="KPK72_000159"
     CDS             64408..65958
                     /locus_tag="KPK72_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2487412.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGIVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLGAY"
     gene            66055..67407
                     /locus_tag="KPK72_000160"
     CDS             66055..67407
                     /locus_tag="KPK72_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2487413.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEVCRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELK
                     "
     gene            67538..68296
                     /locus_tag="KPK72_000161"
     CDS             67538..68296
                     /locus_tag="KPK72_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487414.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDNN
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            68343..69062
                     /locus_tag="KPK72_000162"
     CDS             68343..69062
                     /locus_tag="KPK72_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2487415.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIGWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(69186..70586)
                     /locus_tag="KPK72_000163"
     CDS             complement(69186..70586)
                     /locus_tag="KPK72_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG2487416.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(70590..71498)
                     /locus_tag="KPK72_000164"
     CDS             complement(70590..71498)
                     /locus_tag="KPK72_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2487417.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            71759..72406
                     /locus_tag="KPK72_000165"
                     /pseudo
     CDS             71759..72406
                     /locus_tag="KPK72_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            72731..75370
                     /locus_tag="KPK72_000166"
     CDS             72731..75370
                     /locus_tag="KPK72_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2487418.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTKFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(75428..76531)
                     /locus_tag="KPK72_000167"
     CDS             complement(75428..76531)
                     /locus_tag="KPK72_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG2487419.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKNIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            76891..77712
                     /locus_tag="KPK72_000168"
     CDS             76891..77712
                     /locus_tag="KPK72_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2487420.1"
                     /translation="MGYKLIITDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    77791..78002
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            78089..79405
                     /gene="argH"
                     /locus_tag="KPK72_000169"
     CDS             78089..79405
                     /gene="argH"
                     /locus_tag="KPK72_000169"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG2487421.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            79654..79992
                     /locus_tag="KPK72_000170"
     CDS             79654..79992
                     /locus_tag="KPK72_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG2487422.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(80119..81408)
                     /locus_tag="KPK72_000171"
     CDS             complement(80119..81408)
                     /locus_tag="KPK72_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2487423.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            81488..82192
                     /locus_tag="KPK72_000172"
     CDS             81488..82192
                     /locus_tag="KPK72_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG2487424.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            82284..83330
                     /gene="selD"
                     /locus_tag="KPK72_000173"
     CDS             82284..83330
                     /gene="selD"
                     /locus_tag="KPK72_000173"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:82332..82334,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG2487425.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            83348..84760
                     /locus_tag="KPK72_000174"
     CDS             83348..84760
                     /locus_tag="KPK72_000174"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG2487426.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            84874..86757
                     /gene="selB"
                     /locus_tag="KPK72_000175"
     CDS             84874..86757
                     /gene="selB"
                     /locus_tag="KPK72_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG2487427.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            86862..>87578
                     /locus_tag="KPK72_000176"
     CDS             86862..>87578
                     /locus_tag="KPK72_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG2487428.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIII"
BASE COUNT        33828 a         9964 c        14875 g        28911 t
ORIGIN      
        1 gttttaaaaa ctttaataaa atattttttg tttttagcat aaatagtata agtaagtttt
       61 gtataatttg ttattatttt gtattgatta taattattat acaataaaga ttctgtctca
      121 aaatagagag agaaacctgt ttttcattga taaaatgata aaaatatatc tgatttcata
      181 caaaaaagag tgtctcatga actaaaaagt tcattttgag acaccacctt tattatttta
      241 tattatttat ctcttgtata tattactata gcatccctta aaaacttagc taatccaggt
      301 tgttctctat cataatatga tgtaaaacgt tcatcatata catacatctg tgctagagag
      361 gaatgagctt ctttagagta tgtgttccaa gtgtaactca accatttatg atgtaatttt
      421 gcaacttcct gagccagttc actagaggca tcaccagttt taaaagcttt tttaagttta
      481 ctaattattt cgatatttag gttttgccaa ttttcataat cttgcttaga catattttta
      541 agttttttat ttgatgtatc tataatatct ttaccatatt tctctctaat ttcagttcca
      601 taattttttt cattttcatc aatcatcttt tctttaaaac cttcaaattt ttctatatct
      661 gtcatagtgt attttccctc cttcagagct atagttttag ttacattttc tattagtaaa
      721 tcaatttgct tccttttggc tagtaattta ttatggtgtt cttttaacgc acttaaacta
      781 tcgaagtttg gtgaatttat aatgtttttt atattttcta agttaattcc taattctcta
      841 taaaaaagta tttgttgaag tttattaact tcattttgac catatatacg ataacctgaa
      901 gaatttattt taagtggttt aagaagacct atttcatcat aatatctaag tgttctagta
      961 cttataccag ctagtttact taatttttga actgtatatt ccataagtag tacctcctat
     1021 aaattaaata tacactttta cgcaacgtaa atgtcaatac attaaaatat aaaaatttat
     1081 atcttttaat aaaaatataa aaaaatatct ttactggata ttttacacac aactgtcaat
     1141 actcactaat gaaaattaaa aaataaaatt tatagactat ctttactaaa aaatagaggg
     1201 tctatagtct aggaggaact gtatgaattt aaaaagaagc aatgaaaaac gagaagagta
     1261 cagaagaatg tattcagata aagaaagttt tttaagtttg attcaaaatt ttacaagtgt
     1321 ctcaatagca aaagaattaa ctcttaaaaa tatagagtta gaaacatctt ttatatgtga
     1381 atataaagga aaagaaattg atataattta taaggtattt tctaaaagcg gcaaagtatc
     1441 acattatata gtgttagaat ttcaaacaga aatggatact gaaattgtcc caagattgaa
     1501 atcgtataga gaacaaattt ggaaaagttt tattatgaaa aaaagtcttg aagaaataga
     1561 agataaaaat tttaaacttc ctaaagttat accagttgtt ttatatagtg gacctgaaag
     1621 gttaagtaga aaaaaaggaa ttttggatat tgtagaatct atatcggagt tcagtaataa
     1681 ttctagtatg aaaaattaaa attacataat gtgtttattg gtctaaaatg gaaaactgtc
     1741 gcataaactt gaatatgaaa atatttatag atacaggttc tatgtggagg tgttccatga
     1801 gaaataacaa gtctaaggta ttgctaggaa tactttgtct agcaacagga atggctgtaa
     1861 ttctgtcaat gttgcttcct ggctggattt ggtcagcact taccgcatta atacttattg
     1921 gatgtggagc attattattt ttttgttaaa atagttttaa ctataaagtg tggggtgagt
     1981 attgatgaaa gtagtaacaa taaaattacc aaaatggatt tcaagtataa tcctaaaatt
     2041 tatgagaaag agtaaaaatg attaaattaa aaagcctact tacggaagta ggcttttaaa
     2101 cgtggttact aagctctttc aattttacca gaacgtaaac atctagtaca aacttttact
     2161 ctcttaggag ctccatctat aatcgctctt acgcttctta agttagctga ccatgttctt
     2221 ttattatgtt tatttgagtg tgatacttgg tttccagaaa ccttaccttt accacatacg
     2281 ctacatactt ttgccatcta cgcacacctc cttaaaatga taactattta aagactaaaa
     2341 acatatttat attatcataa ataacaaaaa tttgcaatag aaatatatat ttgccttaca
     2401 catattgaag aatttcttat tatcatataa aattatatat ataattttat ataaagtaaa
     2461 tatatttgga aaataattat tatttttctt tgaatatata tactatagaa gaaattttat
     2521 ccaaaaactt ttctaggttt aaattatacc atatttatag tatgatatga ttataatttt
     2581 atataataaa taagtaaaaa tagtaaaaaa taaattaaga aatgttttta ataaaaataa
     2641 gcttaagggg gtcaaccatg agtgcaaagg taacgaatca atatggaaat atagaaatag
     2701 ataatcaagt aatagctcaa gtcgtatata gagctgcaat ggaaagttat ggcttagtag
     2761 gtttagcata taagtctaaa ggaatagtag agctattaaa aggcgagaac gcaactaaag
     2821 gtgtaagagt tgaggaagta gaagaagatg ccatagcaat cgaactttat gttataatac
     2881 aatatggtac aaatatatct actgttgcca ataatataat agatagagta aaatatgtag
     2941 ttgaaaaaat gactggcgta agagttacta gaattgatat taatgtacaa ggaataagag
     3001 ttaagtaatt tcataggagg aaacaaatga ttcaatatat agatggtaaa aggttaaggg
     3061 agatgtttat ttcaggtgca aacaatctcc aaaacaataa agagttagtt gacaagttaa
     3121 atgtattccc tgtaccagat ggagatacag gaactaatat gtcattaaca atatcttatg
     3181 ccttaaaaga attagcaaaa gttgaaaatg ataatatatc agatatcgga aaagcattat
     3241 ctaaaggttc tttaatggga gctagaggaa actcaggagt tatactttca caaataatca
     3301 gaggtatagc taaatctata gaaggaaaaa gtaaattatc aacagaagat ttagcaaaag
     3361 cttttaaaaa tggttcagac acagcatata aagctgtaat aaaaccaatt gaaggaacta
     3421 tacttactgt agttagagaa agtggagagt ttgcaataaa aactgcgaaa aaagagaaag
     3481 atgtagtaaa gtttttaagt atgcttgtga aggaatcaaa ttcttcatta gaaagaactc
     3541 cagaccttct aaagaatcta aaagaagctg gtgtggttga ctctggtgga aagggtcttg
     3601 tacttatata tgaaggaatg ttagcttcta taaaaggcga taacatagaa ataaaaaatg
     3661 ttgacctaga tactaatatt tcaacaagta tggattttgc aaagagtact actagcacag
     3721 atgatataaa atattgctat tgtacagaat ttattttaga aagttctaag gtagaagata
     3781 ctaaaataag agacataatg atggcttatg gtgatagcct agcagtagtt ggagatgatg
     3841 gagtcataaa ggtacatgta cacactaatg acccaggaaa tgtacttcaa gaagctctaa
     3901 agtacggtca attattaact ataaaaatag aaaatatgaa gttacaacat gaaaatacat
     3961 tactggatgt agaagaaaag aaagaaaatg atagtgaacc tttagaagaa gaaaaagagt
     4021 ttgggtttat agctacttct atgggtgaag gtttggcaaa tatatttaaa gatttcggag
     4081 ttgaccatat aatagaaggt ggtcaaacta tgaatccaag tactgaagat tttatgaatg
     4141 ctataaagca tataaatgct aaaaatatat ttattttccc aaataacagc aatataataa
     4201 tggctgctaa tcaggcaaaa gaattgagtg ataaaaatat aatagttata cctacaaaaa
     4261 atacgcctca aggatttgct gcattagtaa cttttaatgg agaattaagc gaagatgaaa
     4321 ataaagaagc tatgatgaat gctttaaact cagttaaatc aggtcaagta acttttgctg
     4381 ttagggatac agtgatgaat gaaattgatg ttaaagaagg taatataatt ggaattgcag
     4441 aagggaattt attatcagca ggagattatg tggatgaagt aacatcaaac cttattgaaa
     4501 agttggttga tgaagatact gctataataa cattattctt tggtgaagat gttacagaat
     4561 ctcaagcaaa tgaactacgt acttctttag aagaaaagtt tgaagatgta gatgtagagt
     4621 tatattatgg aggacaacct ctttattatt acttaatttc agttgaataa tgattataag
     4681 aacctgtatg accaagtagc ttttataaaa ttggttgtac aggatttttg tgtagtaggt
     4741 gatatatttg ttggatttat ataaagatgt tcaatatgtt aagggaatag gacctaaaaa
     4801 agcagataaa ttaaataaac tgggtatatt tacactaaaa gacctattgt attattttcc
     4861 aagacaattt gaagatagaa ataatctaaa gaaaatagca caattagaag atggtgagaa
     4921 agttacgata aaagcagtaa tatcaagtat aaatacattt agcccaaaag aaggtatgac
     4981 tttaacaaag attgatgtaa aagatgagac aggctcggct aaattagtgt tttttaataa
     5041 gagttatata aaaaatactt ttcgtccagg agattcaata ttagtttttg ggaaggttaa
     5101 gaaaaaattt aataacttag aacttacatc atgtgaatta gaatatctta ctaattcacc
     5161 taagaataca tgtagattta tgcctgtgta ccagcttaca tacggtgtta caaataaaga
     5221 aattatgagt ataattagaa cagtacttga agataaagaa ttaattatac aagaatatat
     5281 gccacaaaga attatagaaa aatataggct atgtagtata gattttgcag ttagaaacat
     5341 acattcacca agtagtaagg aatcattaaa aatagcttta tatagaattg tatttgaaga
     5401 attacttata ctacaactag ggttgtttgt atttaaaagt ggcagaaata aagaagatgg
     5461 aataaagttt gaaacaagta aagacttaaa aaaaataata agtgttttac catttaaact
     5521 gactaaagct caaaacaggg cattagatga aattatacaa gatatgaatt tagagaaaat
     5581 aatgaataga ttggtacaag gtgatgtagg gagtggtaaa acagtagtag ctctcttagc
     5641 acttgcaaat tgtgttttaa atggttatca gggcgctttg atggcaccta cagagatatt
     5701 agcagggcaa cattatattt cacttactga gtctctaaaa gattttggaa taaatgtagg
     5761 tctattaata ggtagtctta caaaaaaaca aaaggataca gtattggaac aaataaagaa
     5821 taatgagatt gatatattaa taggtacaca tgcactaatt gaagataagg ttgaatttaa
     5881 taatattggt ttagttataa cagatgaaca acatagattt ggtgttatgc aaaggagcaa
     5941 actttcattg aaaggagcaa atcctgatat attagtaatg acagcaacac caataccaag
     6001 aactttagca cttatactct atggagattt agatatttct attatagatg aacttcctcc
     6061 aggaagacag ccaatagaaa caattgctat tgaaaaaagt aagcgagata gagcatataa
     6121 taatcttgta agaagagagg ttgaatcagg aagacaagtg tacattgtat gtcccttggt
     6181 agaagaaagt gaagccatag aagccaaatc agcagttgaa cttgttgagg agttaagagc
     6241 ggaatatttt cacgacttaa ggcttgggtt gcttcatggt aaaatgaagt caagtgaaaa
     6301 agatgaagta atgagacttt ttaaagataa agaaatagat attttagttt caacaacagt
     6361 aatagaagtt ggtgtcaatg tgccaaatgc aacattaatg ataatagaaa atgcagaaag
     6421 atttggactt gcacaattac atcagcttag agggcgagtt ggaagaggta gtcataaatc
     6481 gtattgtgta ctaatttatg attctaaaac agatgtatgt agacaaagaa tggctataat
     6541 ggaagagacc aatgatggtt ttaagatttc agagaaggat ttagaaataa gaggacctgg
     6601 ggaatttttt gggacaaggc agcatggtct tccagaactt aaggtagcaa acttatttaa
     6661 gcatataaaa atactgaagt tagcacagca agaagctcga tatatattgg gtgaagataa
     6721 taatctacaa ttaaaagaaa acatggcact caaaaaagaa attatagata aatttaaaga
     6781 tacattgaaa gaaatttcat taaattaatt ttaaattgtg ttaaaatatt actaatgtaa
     6841 taatgtgctg aagggagatt gtcatattga gagtaatttc aggaaaagcg agaggattaa
     6901 aactaaatac tccaaaaaat gaagatgtaa ggccaactac agatagagta aaagagtctc
     6961 tatttaatat gataaattca tatataatgg aaagtgaagt tctggattta tttgctggga
     7021 ctggttcttt aggaatagag tgcttatcta ggggtgccaa gtcatgtact tttgtagata
     7081 tcagtaagga aagcatagat attgttaagt ccaatataaa aaaggcaaga gttgaaagtg
     7141 aaagtattat tttaaacctt gattttaaga ctgcaataga taaacttaaa ttacaaaata
     7201 gtaaatttga cataattttt atggaccctc cgtattataa aaatatgttt atagaagcta
     7261 ttgaaaaaat tgataattct aatctattga acgaagatgg aataatagtt gtagaacatg
     7321 atacgaatga tttatttcca gataagatat gtaaattgga gaaaactaga gataagaaat
     7381 atggaaatac tacattaact ttttataaga tggaggcata atatggaaaa taaacctaga
     7441 aaagctatat ttgcaggaag ttttgaccca attactaatg gacatttaga tattatttgc
     7501 agggcttcga agttatttga cgaattacaa ataggagtat taaataatcc taataaaaag
     7561 ggtctattta gctttgatga aagagtaaaa cttatagaaa aaagtacaag ccatttaaat
     7621 aatataaaag ttgtgacttt tgatggttta ttaataaatt actgtcagga aaatgggatt
     7681 ggagcattag ttagaggtgt gagaagtggt gctgatgttg attatgaact tcagatggca
     7741 catatgaata gagagcttaa tccagatata gagactataa ttttaccaag ttgtacaaaa
     7801 tattcattta taagttcatc tttaataaaa gaggttttgc tctttgatgc tgatataaaa
     7861 aatttggttc caaagattgt tttggaagaa ttaaagaaaa aaactagtgg gggtaattga
     7921 tatatgaaga tagatttaga aatgattgaa ctatttgagc agttagagga gatatttaga
     7981 agtgcttcta caatcccttt ctcacataaa tgcacagtgg ataaagatga ggttttagca
     8041 ataattaatg atataagaac tttaatacca gaagaagtaa cacaggcagt atggattaat
     8101 aaagaaagaa ataaaataat aagtcaagct aagcaagatg ctacaaatat agttgaacaa
     8161 gcgaaaaaag aagctgagag aatacaacaa gaatatcaag ataatataga ggaattaaag
     8221 aaaaattctg aagatgtagt aaaggcatat gtagaatcca gcgaacctgt tgttcaagca
     8281 gaccaaagag caaaagatat agttgataga gctgagagaa tagccaatga gataagaatt
     8341 gggtcaattg aatatgcaga ggatgtgctt tctagtgttg agtataattt aagagaaata
     8401 ttagaagaag taaaaagaga taaagctgaa cttagaccta aaaaataaat tttaaataaa
     8461 taaaaatgga gctatcacac aaaaaattcc aatttatgtg tgatagctct cttttaatat
     8521 agcaattttt tgttatataa ttgtgctttt tagattactt atatcttatt ttgatattat
     8581 aatgtagttt aatcttttat ttgttgaaat caacttattt atttgtataa ataatatagg
     8641 ttattttcaa atttttttaa taattttacc actgtcttct tttagattag agttagactg
     8701 tgcaaaatta taaaataata ctaaatcatg tgcaattatt tggtatggca ctacatgtag
     8761 aaaatataga taataaaatt aaggaggtag tatgaaggaa agggctaaaa aaatattaga
     8821 ttttattatt aaaaataaca atgtaacatc acaagaacta caagaaaagt ttaatgtttc
     8881 aaagagaact atttattacg atattctagc tataaataaa caactaggaa aaagtggtaa
     8941 tataaaaaat gtaaaacaca aatttatatt tgaaggtaat ttatgtgatg caaggaaaat
     9001 aatttcaact gaagaagata aatttttaga tagtgattac agaaaaactt ttatattaaa
     9061 caagatatta cttggcgaaa aaatatcaat agaaaaacta acaaatgaaa tgttactttc
     9121 aaaaaacaca gtagtacaaa caataactga tgtaaaaaaa taccttcaaa caatggggtt
     9181 aagattagag tataagggaa agtacaaaat tataggtgat gaatacgtta ttagagagct
     9241 gtttctgata attgttcaag aaaatgtact ggaaattaat tctataagtg aggaagtaag
     9301 tagtttcgat acaaaaggac atataaaact tactgattat tctttattaa atctaactaa
     9361 gtttgtggaa tttctaaata aaaggataag agatgggaaa acattatact catataaata
     9421 tctaaatgaa gctaaaaaaa ttagttactt tagtaattgt aaagaacttt tatgtgaaga
     9481 agctaatgaa aatgaacaag cctatatatg cacttacata agttcacttc ccagcttaaa
     9541 tagtgaagtt aaggaagatg ttgttgaaga atatgtagat aaacttatag ataagtttga
     9601 agtaaataca gcaataaaat tagaaagtaa gcatgagttt aagaaaaata ttttaaggca
     9661 cctccactct tcttacaaca gaatacgatt taagtttcct attagaaatc caatgttaga
     9721 tgagactaag tataaacatg agtcacttta taaaattata aaatcaatta tagagaatga
     9781 agaagagttt cctgtatttg aggggataag agaagaagaa ataggattta tagcagcgta
     9841 ttttggagga tatttaagag ggtctaggga taatggtcta agaagaaata aagtcttact
     9901 agtttgtcct aatggtctta tggtatcaaa gagtttggaa atacaactat ataaatacat
     9961 accaaccata gaaatagtag gtatagtatc cattaagcaa ctaaaagaag taaatgtata
    10021 ctacgattac ataattacaa ctatagatat acaaaatgta aacaacgtaa ttgttgtaaa
    10081 tcctctttta acatcatcag atgttcaatt acttatgaac aaactaataa gtgtaaaaga
    10141 aaacgagaag tatttcaatc ttgaattaat aatacaagct ataagaaaaa atggagttat
    10201 caataatgaa gaagcactaa aagctgattt attaaacata atacacaaga tagatgaagg
    10261 agagatgtat caaccaatgt taaaggaatt aattaatgca gaaagggtca atataataaa
    10321 aaatgttaga gattggaaag aagcaataaa aatagcctca aagccattgt tagaagataa
    10381 ctctatagaa gagttatata tagaaaatat gataaagtca gttgagaaat atggaccata
    10441 tattgtttta gctgatagat ttgctctacc acatgcaagt tctaaagaag gagtaaacaa
    10501 gcttgctatg tctttactaa tagtagaaga tgaagttgat ttattaggaa aaccagtaaa
    10561 tatatttatg gtattagctg ctgtagataa cactactcac attagagcat tagcatcact
    10621 atcagagatg atgtacgagg aagaaaatgt aaaactaata attaatggtg ataaaagttc
    10681 aatactagaa cttataaata agcaaaatta ggaggaatga aaaatgaaga tattagcagt
    10741 atgtggtttt ggtgtaggtt catcaatggt attaaaaatg acaatacaaa aggctctaaa
    10801 gcagttaggt atagaatgtg aagtagaaaa tacagatata aattcagcta gaggtgctga
    10861 ttgtgactgt atattcacaa gtcaagaatt gttaaatgaa ttaaaaggaa cttgtaatgt
    10921 agatgtatat ccagttaaaa agtacatgga tttagcagaa gtcacagaag ttgttcaaac
    10981 ttttttaagt gataaaaatg ataagaaata gtataagttt taaaaagtaa atttaagtta
    11041 atcagaataa aataaaaact tactaaattc aaaaatagca agaaagagag gagggattta
    11101 gtggtagaaa acttaaacag ttgacctatc actaaaaaat ataatgcttg attttatttc
    11161 aaataatatt ttacgtaatc catctatgtt aattggatta atagcagcat ttggtcttgt
    11221 actacagaag aagaaagcat ctgatgttat aaaggggtca ctacttgcag ctttcggaat
    11281 tataattatg gagactggaa caggtatgtt agttagttca atagcaccaa ttaatagagc
    11341 tttccaaagt ataagtggtg gtgcagtaac aaaaggttta agtgatgtta cttttactgc
    11401 aagttatggt ggaattatag gtttagcaat gtttgtgggt ttagctattc acctaatgat
    11461 agcaagattt actcctatta agacaatctt cttaacaggt cacatgcttt attggttccc
    11521 attcatattt gtagcttcag gagttgaagc tggattaaat ggtggagtat taatagcttt
    11581 tgcagcagta ttatcagcac tctattggtc aataatgcct tggttattaa gaaaatatgt
    11641 gtttgatgtt ataggagatg attcttttac attaggtcat ccatctggtt gtttagcact
    11701 tatatcagga tttgttgcta aattagtagg aaataaagaa aaatctacag aagatataaa
    11761 catacctcaa agtttatcat tttttagaga aatatctata agtggtggaa tggcagtgtt
    11821 tttaatgttt atagttgttg ggatagtaat accaggtgcg tttaaagata gtaatgagtt
    11881 tattggaacg tatgcaataa agcaaggtct ttcttttgga gcaggtctta taataatgct
    11941 tcatggggta agaatgctaa taaatcaaat aatgccagca tttcaaggta tatctgaaaa
    12001 agttgtgcca aatgcactac ctgcttttga ctgtccaata ttatttaatt ataaaccaaa
    12061 tgctgtatta ataggatttt tagttgctat gctaacatct acagctatga ttttaatatg
    12121 taactacttt aatgtattta atttgctact tataccactt gttataactt cattctttga
    12181 gtgtggagca gcagcagtaa taggagaatc acaaggtggt atacgtggag ctataatagg
    12241 tacagctata gctgcaatat ttatggtagt tttagtagga atatctgctg taatttactc
    12301 aggaactatt caaaattgga tactaatatt tggaggtaat gacctttcat tatttggtat
    12361 aatcagtagg tttataggtt cactatttgg aggaatatta tagatatgaa atttaaatac
    12421 atagatgaga taagaagatt aattgatata atagaaaacg aagaaaaagt caatatggaa
    12481 aaagcagtac aaatctttgt tgaagcaata gaaagtaaaa atgctatatt ttcatttgga
    12541 gcttcacatg caggaatatt aacagaagaa ttattctata gagcaggtgg ctgtgttctt
    12601 ataaatccag tatttgcaaa atctctaatg ttagatacag cgcctataac tcacacaagt
    12661 aagatggaaa gattagaagg atatggtaca gctctagctg ataaaactcc aattaaaaaa
    12721 ggagatgtag ttatagttca ttcagtatct ggtagaaatc cagtaagtat agagtttgct
    12781 atgaaagcta aggaaaataa ggcaatagta atatgtataa ctaatttaag ttactcaaaa
    12841 agtgtatctt caagacattc ttcaggaaaa aaactttatg aagtatgcga tttagtgata
    12901 gataatcatg gtcaaaaggg agatgcctgt gtagagatag atggattaaa ccaaaaagtt
    12961 ggtccaactt ctactgtaat agcaactact gtgatgaact ctattatagc tcaagctact
    13021 caggaattag ttaataaagg gctaaagaat cctccgattt tctatagtgc caatattgat
    13081 ggtggagatg agttaaataa gaagattttt gatgaatata aaagtgtaat tcattatgaa
    13141 tattaaggtt aaaaaaatat gttaatgaac tttgaagttt atcaacaaag aatattaata
    13201 atatataata aatgctatca tttaaaagct gtaggtaaaa atctacagct ttttattatt
    13261 gtagattaaa aaggtgcgta attaaatttt aaattggaga aaataaattg gaaggtatca
    13321 attataaata ttagaaacgt tgtcatagaa aaaaactata ttaaaaaaaa gtacatagaa
    13381 aaaactgatt agacaataaa tacatgcttt gataatattt taaatggagt gaaaatgtgt
    13441 agaattataa tgaaaaaaga aaataaaaat aaaaaaagaa aataaaatgg agggaataaa
    13501 agtgggtatg ttaaaaaaat tatgttcact agctatggta gcaattatga ctacaacact
    13561 tataacaggg tgttcaagtg gaaatggaaa agattcaaaa aaggatggcg aaaaagaaaa
    13621 actagttctt tggatgccac cagtagaaaa taatatgaag gaagtttggg accctatttt
    13681 agataaattt gaggaaaaaa ataactgtga agtagattta cagataattc cttgggataa
    13741 ttattctgaa aaatttgcaa cagctataag tgctggcgaa ggtccagatg taggatatat
    13801 gtatgcagaa atgtatccac agtttataga aagtggagca gtagaagatt tgactaatta
    13861 tgcaactaaa gaggataaag agcaatctat ttatataaaa acatctgaaa tgatgggtgg
    13921 aatgtatggt atgccattcc aagcagcaaa tccaggggta ttatactata acaaggatat
    13981 tttagagaaa ttaggagaaa aacctcctaa aacatgggaa gattttaaaa gaatttgtaa
    14041 aaaagcaaca caagatactg atggtgatgg aaagatagac caatggggat tagctcaggg
    14101 atggggagct aaaacttttg gtaacttaaa ctggaactgg tatccttatt tatggcaagc
    14161 tggtggagat atatttaatg atgatttaaa atcggttaaa tttaatgata agagtggttt
    14221 agaagcagct aatttcttaa aagaattaca agcatatgtg cctgaagatt ctttatctaa
    14281 agatagtaat gaaatgatag aaagcgtatt tggaccaggg aaagcagcat ttacaataat
    14341 gttatcttct gctgcaacaa gtgtatttga tgaatcattc ccggatttaa attggggatt
    14401 tgtaactgga ctagaagata agaaagcagc aacttttgga gcagtagacc atttatcttt
    14461 aatgtcatct gctaaaaata aagaacttgc atacaagtta atacaacata tgttaagtgt
    14521 tgaatcaatg acagaatttc ataaagcaat accaagagca ccaataacaa aaggtgaacc
    14581 atatcaaggt gatgaaagat tcaaagaaat ggtggaaaat gacaaaaatg tatatagacc
    14641 attagtagta ggacctcatg gagttgaaat atatgaatat ttatggaaag aacttcaaac
    14701 tatgatttca ggagataaaa ctccaaaaca agcgttagat gatgctgcaa aatattcaaa
    14761 tgatttatta gcacaataaa agtttaaagc tgtaatatgt tgtaaaggac tttacagatt
    14821 aaaattagca ttgtatttat aaatacaatg ctaatattta tattggagag gataaaatgg
    14881 aaaacttagt tagagagttt gaagaaaatt caaaaagaaa aaaaacaaaa ttaaatatta
    14941 aaccatatat gtatatattg ccattgggaa taattttagt ggcgttttat gtgataccaa
    15001 taataatgtc aatatacttt agttttacaa agtataatat tattagtcca gctactttta
    15061 taggtttaga aaattataaa aaattattta cagatgaaac attaaaagta tctattataa
    15121 atactattaa atttactgtt gttgtagtac cttgccaaac tatattatca ttgattttgg
    15181 ctgtttggat tacaggaaaa ggaaatagta aaatagcttc atttgctaaa ggtgctatat
    15241 ttataccagt attatcatct atggttttga taggtatggt atggagggct ttattgaatg
    15301 gagaaggttc tattatatat caggtattag gtgcctttgg aatagaatca tctaaacttt
    15361 taggagatag taagacagca ttacctacac ttatgtttat atctatgtgg aaaaatatag
    15421 ggtatttcat ggtaatttat atttcagcta taatgaattt accaaaacac tgctatgaag
    15481 tagcgaaggt tgatggggct acaaaacttc aagaatttat aaaaataact gtacctttat
    15541 tgaaaccaac tacaataatg gttgtatttt tagggtcgat atggtcatta caagtatttg
    15601 acttaattta tactgtgaca ggtggaggtc caggtatatc tactatgagt atagttatgc
    15661 atgcatttaa cttaaacttt aaaaacttta attctggata tgcaatgact gtagcaaacg
    15721 tattattttt gttaatagca gtagtttcga ttttacaaaa taagcttgta agacgagaca
    15781 attcagattt ttagaaaggt aggtataata tgaaaacagc aagaagatta ttaagtgatg
    15841 gtttactttt atttatagcg tgtgcaagtt tggtaccttt tatatacatg ttgataatat
    15901 cattgaagat aacatataac tcatatagtt tggatatatc tttttcaaca gtaacccttc
    15961 aaaactatat agatattttt actaaaaaag gatttgcaca atattttttt aatacagcaa
    16021 tagtatcttt ttcaggtgta ttattaaatt tagtatttag tacattagca ggatattctt
    16081 ttgcgaagat ggattttaaa ggtagtgata agttattttt atttatgata atgactttaa
    16141 ttataccttc ccaagttaca atgataccac tatatataat aatgaaacac ttaggatgga
    16201 taaatagtta tcttgcactt attatgccaa taccaactgc atttggcgta tttataatga
    16261 gacaagctat tttaggagta ccaaaagagt tattagaatc agcaaagata gatggttgct
    16321 cggattttag aattttaata caaatagtgt tgcctttaat taaaccagca ttaattacac
    16381 tagcaatatt tacttttatg ggagcatgga acgagtttat gtggcctctt atagctacaa
    16441 ctaaagatgc aatgagaaca ttaacagttg gattatctac tcttaagaca ttccagataa
    16501 ctaattatgg acagatgatg gcaggggcta caataacatt tttacctcca tttattttct
    16561 atttgatttt acagagtaaa tttgttgaag gtgtgtcttt gtctggtata aaaggttaaa
    16621 tagtctaatt taataatata taaagaaact tatataaata tggaggaatt taaactatgg
    16681 aaatttcata tgtactgaaa gattgcacat tcaaacacaa taaatactca gttgtagaag
    16741 ctgaaacttg gaaagataag gatttggata ttgtagtagc taaaggtgaa aaatttgcat
    16801 ttcaaattat gttaaaagca acagaagaat ttaattgtac aattgataaa agtagtgcta
    16861 tatcatggaa aggcctggga aatagagtaa gattagcatt aaatgtacct aatagtttag
    16921 aaaataattt tagtataaat atactaggat atgttcaaga tgatacaaaa gcatttgtga
    16981 atgatgctat tttaagggat aaagatatgt tagtagagca atacttgcct caaacatttt
    17041 gggtcgaagg tcaagttccg gaagattttg aagaaaataa ctttaatata ggtattgata
    17101 tatttaaaag ctttggatat gaagatgaag aaaaagcttg tacaattgac gttcctgtag
    17161 aagtaaaaaa tgttgtatta aagcctttag atgaaagtaa gttcttttta gatttatggc
    17221 aacatccaag ctgtctagct agaatgtata aagttgagct atggtctgat atacattttg
    17281 aaataatcga taactatcta aaagaattag cttctttagg agaaaaagtt gcaacaatta
    17341 tagtttcaga ttatccatgg gcagggcaaa gttgttataa agtttataaa aatccatcaa
    17401 acttatatga atataacatg gtaagtgtat caaaaggatt ggatggaaag attaaatgta
    17461 actttgagag tatggatagg tatataagca tagctgataa gtataaaatg gctaaggaga
    17521 tagatttatt tggtctatta ggtaattggt gtgctggtga gtttggaaat cctgtagaag
    17581 gttataagga ccctataaga gtaagatatt ttgatgaaaa ggataaagta tttaaattta
    17641 taaacaatac aaatgatttg aaagaatata taggattagt attaaatcat ttaatagagt
    17701 gtggattatg ggacagagta aggattatag ctgatgaacc aaataatcca gaagtagtca
    17761 aagagtgtat agaatttata aattctactg taggtactca tcaagttaaa tataaatcag
    17821 caactcacga ccaaaacttt ttagacagag ctaaagatga aattgatgat atgtcaataa
    17881 atttaaaact tactatacaa aattataaag atatagaaag tcttaagaaa aaaatcaatg
    17941 acaagggtgg gatattgact tggtttgtat gctgttttcc agaaaaacca aatagttttt
    18001 taagttctcc ttttgtagaa aatagaataa taggatggta tacttattac tttggtcttg
    18061 atggattttt aagatgggac tataatttat ggacagaaga cccttggaaa gattcaagtt
    18121 acaaattccc tatttggaaa gctggagata tgttctttgt ttatcctgga aaagatttaa
    18181 agccagttag aagtgtcaga atggagaatt taagatttgg aatacaggat tttgagttat
    18241 ttacaatgtt agaaaaagaa aagggaagag agtatataga agttgagctt atgcaagaat
    18301 tattaaataa aaaagaaaat gctgaaatta aaggttttgg tgacatagaa ctaggatatt
    18361 cacttgataa ttggggatat gataaagtta aaaaatatgt attagagttg ttagacagag
    18421 tataaaaaat ataagagtgt ctcaaaatga actttttagt tcatgaggca ctctttttgt
    18481 atgaaatcaa tatagtttca ttgtttcaac aataaaaaat aggattattt ctattctaag
    18541 acagcccttt agttttttag ttgtattcaa tgttttaaaa ctaactataa acgctataaa
    18601 gtttataaat gacaataatt atatgtaaca tgatgtactt taatttgcta taatagggat
    18661 tgaataatat tatatggaag ataacattgt ttggggggat taatataaaa agaataggta
    18721 ttattataat gtttttttta agtatatctt ttattggatt ttatcttgta ataagagata
    18781 acagtgatac agttaagatg caacgaggga taaatatagg taatgcacta gaatcaccta
    18841 aggattttcc atgggatgta aaaatgtcaa ataagttttt tgatgatata aaagatgcag
    18901 gttttgacac agtacgtata ccagtaaggt tttctgatta tacgtcagat tctaataatt
    18961 ttaaaattga tgaagaattt tttaaaaaga ttgataaata tgtagattat gcattggata
    19021 aagacttaat agttgtgttg gatttacacc attttgaaga aattatgaaa gaaccaagag
    19081 ttcataaaga aaagttttta aaaatatggc aacagatagc taataggtat caaaaatatg
    19141 ataaaaaatt ggtatttgaa ttattaaatg aacctaaaga aaatttatct tcacaattac
    19201 taaacgaata cattgaagaa gcaataaaga taattagaaa aactaacccc aaaagaacta
    19261 ttatagtagg accatataat ttctatcaaa ttgattattt gaatgaatta aatattccta
    19321 aagattctaa tatagtagtt tcttttcact attacgagcc aaatgatttt gcatttcaag
    19381 gaaatatata tcataaggga tttgagcact taagcaatat tacttgggaa ggaacaaatg
    19441 aacaaatgga ttatttaaaa aaaaggtttg atactgtgga aaattgggca aataaaaata
    19501 aggtaaaaat cttcttgggt gaatttggaa taacaaaaga ggcaccagaa gcttcaagga
    19561 gagcttgggt aaaagctgta agagaagaag ctgaaaaacg aaacttttca tgggcttatt
    19621 gggagcttgc atctggattt ggtatataca atcaaattga agggacttgg gatagagata
    19681 tattaagtgc attaatagaa aagaggtgat tttatgaaga aaaattcaaa aaagatactt
    19741 ataactttaa ctattattac taatgtcata tatatattgt ggagaatttt ttacacagta
    19801 cctaaagaag aagggaagtt taccttaata tgtgccataa tacttctttt tgtagagata
    19861 atgggcatga tggaaatgtt tgtgcattat tatggtatgt caaatattga gtatcctgaa
    19921 aaaccaatca taagtgaaga actttatcca catgtagatg tatttattgc aacatataat
    19981 gaatctgttg atttggttag aaaaactgta aatggatgta ttcatatgca gtatccagat
    20041 aagaaaaagg tacatatata catatgtgat gatggaaatc gtgaagaaat gcgtattcta
    20101 gcagaaaaaa tgggtgtaaa ttacataact agaactgaaa gagaaggagc aaaagctgga
    20161 aatttaaata atgcaatgca acatacaaat tcccctttga ttgctacatt tgatgcagat
    20221 atgataccaa tgcatgactt tttaattgct acagtaccat actttttaaa aaatgagcaa
    20281 gctaaaaaag atggagaaaa agaggagtat gaaaaggttg gttttgtgca aactccacag
    20341 agtttttata atccagattt gttccaattt aatctgcact ctgaaggaag gattccaaat
    20401 gagcaagatt atttttacag agatattcaa ttggctagaa ataggacaaa ttctgtaatt
    20461 tatggtggta gtaatacagt tatttcaagg gaagcacttg aagaagttga tggattttat
    20521 acttattcta ttacagaaga ttttgcaaca ggtatcttaa ttcaaagtaa ggggtataga
    20581 tgttatgcta ttccagaagt acatgcatca ggtttatctc ctacagattt aaaaagtctt
    20641 attaagcaac gtgaaagatg ggcacgtgga tgtatacaaa ctggtagacg tctaaatatt
    20701 ttgtttagga gaggtttagg tttttggcag aaaataagtt atatttcttc aattacttat
    20761 tggtatgcaa gtataaaacg ctttgtatat ataatggcac ctattttatt ttctgtgttt
    20821 aatgtgattg tagtaaaatg tacgttactt caagtattgg tattttggtt gccaatgtat
    20881 attttaagta gtttatctct taaaatattt tctcaaaata taagaaatac tagatggacc
    20941 aatatatatg aaactataat gtttcagtct ttaatgccag cagtaatact tgaaacgttt
    21001 gcaatatcta aaaataaatt ttcagtaaca aataagagta aattagaaga aaatagaatg
    21061 tataagtttt tacagggaat accttatttt atttacatgg tcttatctat cataggaatt
    21121 ttaaaaatgt ttgtagcaat atttaaaatg agttcaatga catattcagt tgtattattt
    21181 tggttgattg gaaacctttt taaccttgtt atggctacat tatttatatc agggagacag
    21241 cagttaagaa agtctgagcg ttatattgct gaaattgatt ttaagttaaa gcaaaattct
    21301 tatgttcttt cttcaaaaac aattgatata tcagaaaatg ggtttgcatt tttgctagaa
    21361 aatccagaat atatttcccc agaagaagag tttgaggtag agtttagaga aaaatctgga
    21421 aatgaaatgt atatagctaa tatgaaagca aagattgtta atgttgtgga agtaaattca
    21481 aagtggaaat atgcagctta tattacacat attgaagata gtgaaataga taattggatg
    21541 tgtattgttc acgatagaat acctacttta ccaatgacaa tatctaatca actaggtttc
    21601 tttgatgatt tgcaaattaa tgtaaaaaaa cgtattgaaa aaactagaac attatcacga
    21661 agaagtccaa gaatcaatat gaattttcag atggatataa aaaatacagg taaattaaga
    21721 atagtgaatt ttaattatca atatgtatta ttaaattttg agaataaaag tatttatcct
    21781 aaagaaatag cattggaaat taatgaaaat attgtgttag aatgtgattt gtgtgagggt
    21841 aaaattgatg aaagaggtat cttatataaa gtaaataaca ttgacagcat aatgcaaaat
    21901 ttctttttaa gagatgaaat gatggattgg atattacaaa ataagaaaat tcttgattca
    21961 aagcctagtg aaaagaagga aaaaagtata gatgagtttg aacccatgga atatatttag
    22021 gagagatgaa atgaagactt taaaagtgtt tttttgttct acaaaatatc ttattttggt
    22081 gtttattttt tttataaata ctacttttat aagttcagca acaagtgata aaattttaga
    22141 tttatccttt aaaaagatag agactgactt aagttctaag attacatatg aggatacagg
    22201 tgtaaaaata gaaacggata gttctaaaag cgataaggaa cgttatttat atatttacca
    22261 aaatataaaa gaaaattgga gcatgtataa taatttttat attgaaattc aaaataaaaa
    22321 taaaagttct cagagaataa atttaagcat acaaagtaaa aatatgcttg aatttagatt
    22381 aaaagaaggt agtgaagtat ttttagaagg taaaaatata atatattcag ataaaataaa
    22441 agaaggatgc atagaagtac caggtgaatt tgaaggcaaa atttatgtga actttaatag
    22501 ccttataaat gaggaaagta atgttgtgct ggactcaaat atgctttcaa acattgttag
    22561 ttggggaatt acgtttattc catctgatga agaacataat attgttatta taaaaaagat
    22621 atctttattg tctgaagaga aattaagatt tttaaataat attaaaataa ttggcgatga
    22681 agaagtacaa ataccagtat tgggtcaaag tataagtcag tatgaggtat taggtcttaa
    22741 aagtgattct aaaattaagt actctcttat gggaaaacaa gataatgtga gtatatctca
    22801 aaacggtaaa ttaacattaa gtaataaagc caaacctgga caaattattt tacaagttaa
    22861 tgttgatgat aagtttaaaa ttggaaagaa aataacatta actgaatctt ggagtattaa
    22921 taaaaaagat aaggatggtg ttccatatac attagtatct cctgagcaat caccaacagt
    22981 acaagatatg aaaaaaataa attttatgaa taatatcata acgtttgttc gaattttatt
    23041 tgtatctcta gtaataatat gctttggaat atatttatat tggaaaaaat gttcaaaaac
    23101 taagtaaaga gaggaggtta gattgtgtta ttttcaagtt taattttttt attttatttt
    23161 ttacctataa ctttggtttt atattatgta tttaggttta accgaacgat acagaacatg
    23221 atattgcttg ctgttagttt atttttttat gcttggggag agccaaaatt tgttgttatc
    23281 atgatagttt ccataataat gaactatata tttggtttac tggtagatag atatagggaa
    23341 agtaaaataa aagtaaaagt ttttttagtt ttgatgtgtg catacaatat aggtgtttta
    23401 tttatattta aatatctagc ttttgcgcta agaaatatta gtgaacttat tagtactgaa
    23461 ttgactatac ctagtattgt tttacccata ggaatttcat tttttacatt tcagggaatg
    23521 tcttatgtta tagatgtata tcgtagacat ggagaagtcc aaaagaaccc tttttatgtg
    23581 ggattgtata tagcattttt cccacaatta atagcaggtc ctatagttag atatgagagt
    23641 gtggcagaac aaattctcaa tagaaaagaa acttggaata aattttctat tggtacttgt
    23701 aggtttataa caggtctagg taaaaaagta ttaatttcaa ataacatggc tatagtagca
    23761 gactatattt acactatgaa tagtcaagga gaaatagctg catcacttgc atggcttggt
    23821 tcaatagcct atactctgca aatattcttt gatttttctg cttattcaga tatggctata
    23881 ggcttaggtc ttatgtttgg ttttaaattt gaagagaact ttaattatcc atatatatca
    23941 aaatcaatta gtgagttttg gagaagatgg cacatttctt tgggaatgtg gtttaagagc
    24001 tatatatact ttccgcttgg aggttctaga gtagcaaata aagatattat gattagaaat
    24061 atgtttatag tgtggttatt cactggaata tggcatggag cagaatggac atttgtaatt
    24121 tgggggattt taaactttgt tttcctaatt atagagcgat ttataatgtt tgaaaagatt
    24181 gaaaatcaca attttataaa acatatttat gctttattag tagttaattt tggatgggta
    24241 ttgtttaggt cacctaattt aaaagaagca tataattatt ttaaagctat gtttggaggc
    24301 aatggagcta tatggagtga ttatacatat atgtttttaa aagaatactg ggttttcttt
    24361 atatttgcat ttatttttag tgtacctatt gcaaagaaaa tcaataagtt tgttgttgaa
    24421 caagctaaat atagtatgat atttaacgta ttttatccaa tctcagctat tttattgttt
    24481 tttatttctg tgacatattt agtaactgga agctataatc catttattta ttttaatttt
    24541 tagttgggag gaaaagtatg aatagatttt ttaaagtaag attaataact gcaattatgt
    24601 tcttaattat tttattcgct ttctctatct ttaatgtttg ttattctgga aaagatacga
    24661 tacaggaaat aaaaaattca atagctgaaa aaagtagttt aaaagataca atatcatcta
    24721 ttgataatac agtgaatgac aaagtaatat acaaaaatac gttcattgaa acttatggct
    24781 atttacaaaa attaatgggt aaaaatgagt tttcaaaatt tagtgttgta aaagatactt
    24841 ctggaaagat gcattataca tattttgcta aagctccaaa tccagttggt gtgatagcag
    24901 atagggtaag aaaattttct gatgaaatgg aaaagcaaaa cactaaattg gtttacctaa
    24961 tgactccaga taagtatata aaaggagtga caaaatttcc aaaaggaata ccttattctt
    25021 atagtaatga gacagcagat aatttttaaa atcaactaaa aaagtataat gtggactata
    25081 ttgattttag agaaaatatt ttaaagagtg gtattccaaa agatgattta ttttttaaaa
    25141 cagaccatca ctggaaagta gaaacttctt tttgggcatt tggagaatta gttgagcaac
    25201 ttaataaaaa atacaatatg aatttagatg aaaatcatta ttatagagat aaggacaatt
    25261 ataattcaat tgtatatcct aagtcatttc ttggttctat gggaagaaag actggtatct
    25321 tatatggtgg tatagatgat tttacattaa tttatcctaa atttaagact aattatactt
    25381 attatacaga ttctaaatct caaaaatttg aattaaaagg aaggtttgaa gagagtttaa
    25441 tattatcata tccttttaat gcagaccttg atttaatgga tggtcaatca gataagtatt
    25501 ttacatattt attggggaac agaccattag ttaaaataaa aaatatagaa aatccagatg
    25561 gattaaaagt gctgtttgtt aaagattctc taattgtacc aacagcagca tttttttcct
    25621 ctgtttgttc atctatagat atgatagacc ctagatatta tgatggagat atactagaat
    25681 atgcaaaatc ccatgactat gactttgttt ttatatctgt atatccccaa aatcttacaa
    25741 aagaattttt tccgttttgt gagtaaattt ctaaagaaat tttcttataa aataaaaaat
    25801 aaatggctaa ctggttaaaa gttagccatt tattttaagt gttactctaa gaaatatttt
    25861 ttttactaaa tacttcttta tctatttttc caagtgattt tcctacagta attgatgata
    25921 ccatagcacc attaacattt atcatggtac gtcccatatc tagtataggg tctattgcaa
    25981 gtataccacc aactaatgga aaataagaac caagaccaac tccagagata acaactgaaa
    26041 cggccattgt agcagttcct ggaagcccag ctattccaaa cgaactaatt gttataacaa
    26101 ttaacaacat aacataaaag ctcatatcca tgtttactcc agccatattg gcaagtgtta
    26161 cagccattaa agcaggatat atacctgcac aaccattcat acccatattt gcacctaaac
    26221 ttgctgtaaa gcttgataca ccctcatcaa ctccgtgatt ttgtactaga ctttctatcg
    26281 ttacaggcaa tgttcctaaa ctagaacgtg aagtaaatgc cagaagtaaa gcttcagttg
    26341 aattttttat ataagtaatg ggatttagac catttaaagt tataaaaatt aaatgtataa
    26401 taaataatat tgcaactgat atataaagag ctaatataaa ctgtaaaaca gatactattg
    26461 aactaatacc ttgtgaagtt attgtatttg caagcagagc aaccacagca taaggcataa
    26521 atttaataac agtcatagac acacttacaa ttattttgta agatgcttct ataaaatcta
    26581 taaatggttt tattgtctca aaatgttttt tacttaatct ctttattgag gtagctataa
    26641 atgtagcaaa tattattgta gctacaatat ttccatttgc catagccatt acaggattag
    26701 atggtagtaa tcctcttaaa gtatccacaa gagagtttat ttctttaagt tcttgatttt
    26761 caactaaagt aggcatatta gctcctacac ctaacttcat tatatttgca acgataattc
    26821 caacaattgc agctatagca gtagtagcaa gaagcatacc taaagattta aatgtcagag
    26881 ttccaagatt gttatctttc atatttataa ttacttttat aatggataag aaaactaaag
    26941 gtactactaa catttttaat aagtccataa atccagaacc aaataggcta taccacttac
    27001 ttacttcttg tagccaagtg acttgagttg tatcattagg aaaaccagca aaagcttgta
    27061 ttatgattcc caatatgagt cctagtactg tagcatatat tgttctcaat gaaaatttta
    27121 tttttttctt ttcaaattgt ctaacaataa agaacgtacc tagaagtaat attatgaaaa
    27181 aaattgtttt aaaatctgta atcataagaa attgtgataa aaatgaactt tccaatattt
    27241 ttctcctttt atttaaaaat aaatttttct aagtttaaat gatatcaata tattaaaaaa
    27301 atgtcaatat tatatcacaa tttacttaaa tagagtaaaa caagttgatt ttgaaaaatg
    27361 tgattaagta cagaaaaata gggtataata aatgtttaaa gaatttgaat tgtggtaaga
    27421 aaataaaaaa tagcttttat aagatttgat ttcaaattaa aatatgttaa ataaataact
    27481 aaaaattatt ataaaaaatg ttttcataga atatatatca aatgaaaagc ttaaaataag
    27541 tgaagaaatt aactagacat caaaggagag ataaaatgag agtaataata attggtggtg
    27601 ttgcagctgg tatgagtgct gcagcaaaat taaaaagaat aaaacctgag tatgaagttg
    27661 ttgtttatga aaaaactgaa atagtatctt ttggtgcttg tggattacca tactttgttg
    27721 gtggattttt taatgatgct gatgaattat tagcaagaac accagagaaa cttagagaag
    27781 ctggaattga tttaaatata tttagagaag tcgttgaggt tgatagtgaa tctaagaaaa
    27841 taaaagtaaa aaacataaaa acagatgaaa tatatgaaga ttactatgat aaattgatga
    27901 tagcaactgg agcaagaagt attatgcctc ctataaaaaa cataaaattg aagaatgttt
    27961 ctacattaaa aagtttatat gatggagaat atctaaaaaa acttttaagt aatgaagata
    28021 ataaaagagt gacaataata ggagctggtt ttatagggtt agaagcagtt gaggcatgta
    28081 agaaattagg taaagatgta gatgtaattc agttagaaga tagaatattg ccacaagtat
    28141 ttgataagga gatgacggat gtattagaag aagaaataaa tagacataat gttaatttgc
    28201 acttagatga aatggttgta gaactttgtg gagaagataa agtagaaaaa gttataacaa
    28261 acaaagggga aatagataca gatgtagtaa taattgctac tggcgtgaga cctaataccg
    28321 agtttttgtc aaatactaat ataaaaatgc taaaaaatgg agctatagta gttgatgagt
    28381 atggaagaac ttctgtagaa gatatatact ctgctggaga ttgtgcaact ataaagaata
    28441 tagtaagtaa tgaaaatgta tatgttcctt tagctactgg agctaataaa ttgggtagga
    28501 tagtaggaga aaacctagca ggaagagaag tatcttatca aggttcacta tcttcaagtt
    28561 gtataaaaat tatggatatg gaggcagcat caactggaat tacagaaagg caagctaaag
    28621 atttaggcat aaatgtcaaa tctaaattta tttctgatta taatcaaact cattattatc
    28681 caggaagaaa taaaatatat gtaaagctta tttatgatgc agatacaaaa gttattttag
    28741 gcggacaagt ggctgggttt aaggatgctg tccaaagagc aaatgtattg gcagctgcta
    28801 tctttggaaa aatgaccaca agtcaacttg gaatgttaga tttatgttat gctccaccat
    28861 ttgcaagaac atgggatgtt ttaaatgtag ctggaaatgt atctaaataa tatttagatt
    28921 atagctaata tagttgacaa gcaattaagg gaatgaattt acctaaacta tttttataat
    28981 ggtaaaattc attcctaaat ttttattcca aatttttaga aataaggctt ctattaaatt
    29041 attcatcaaa atttaaacgc aagcttaatc tatcttctcc aagaatagta ttttcaaaaa
    29101 cattagtagc attttctaaa tacatactag gtatgtctaa agaaggacta aaatgagtca
    29161 gtaaaagttg tttgacattt ccaagttttg caagattagc agcttctcta aaagtcatat
    29221 gtttgtttct aacagctttt gatatatcta aatcatcacc atacatggct tcacatataa
    29281 ataaatcact atcttttatg aaatctggta ttgattctat aggtcttgta tcggttgtaa
    29341 gacttatctt aactcccttt ctcaattctc ctaagaccat atctggagtg tattgttttg
    29401 aatctaaaac tatattttga ccttcttgga gtttgttcca taatatttta ggaacttcgt
    29461 ttttagttgc cttatctaca tcaaatttag ggtttcttct aaaattaaaa ctgtatccta
    29521 aacactctat agagtgttct aaactgattg ttgaaatttc taaatttctt aaaactttat
    29581 tatctaaaga aaaattgcct tgagggtttt ctataatttt taaagtgtat ggtacatatt
    29641 ctactaaatt tctcatagaa cgtatacaat cgactattcc aactggacca actatagtta
    29701 aatcacttgt acgaccacta tttccaattg ttgataaaag tcctaaaagt ccaaatatat
    29761 ggtcaccatg taaatgggtt atacaaatca agtctatatc tttaaagcca cattttttta
    29821 gcttcataga tacctgagta ccttcaccac agtcaattag tatttttcta cctttatagt
    29881 ttataaatac agaggataga aatctgtttg gcataggtac atttccacca catccaagta
    29941 aagttaaatc tatcattttt tcccctttct aaaattaaca caaatctgtt gtttgttttt
    30001 attatgatat cctattataa ttattttaac attaaatata acaataaatg tgtatttgag
    30061 tcaagaaata ttgtcaaata aagtttttat atagaaatgt ataaaaattt ggaaaacaaa
    30121 aagtgaaaat tatatttagg agcatttaaa ggaggaaaat ttaatgaaag tattaaagaa
    30181 aattactatt ttgtcattga ttacattaac cttagttggt tgtagtaacg gagaagataa
    30241 aaaagtggtt gaaggttcta agaaaattca aaattctaaa aaagatgaac agcctataat
    30301 agtacataaa gatgcaaata tattgaaaga taggtatgag ccaccaaaag gatttaaaag
    30361 agttgatgta gaaaaaggta gttttgaatc atttcttagg aatactaagc taaaaaatta
    30421 tggagagaaa gtaaagtatt atgatggaag aactaaggaa tcaaaaggta tttatgatag
    30481 tgtatttgat gttgatattg gcaatagaga tttacatcaa tgtgcagatg caattatgct
    30541 tatgagagca gaatacttgt atcagaataa actatatgat gaaataagtt ttgattttgt
    30601 tgatggtttt aaggcgaagt attcaaaatg ggcacaagga tatagaattt cagtaaaaga
    30661 tagtggttca agctggtata aagcaactga atattcaaca tcttatgaga gttttagaaa
    30721 gtttatggat atagtgtttg catattcagg aacgttatct cttgaaaaag aattggagcc
    30781 tgtaaaagtt gaggatatga aaataggaga tgtgtttata gttggtggaa gtccaggaca
    30841 tgcaagtata attatggata tggcagaaaa tgaaaagact ggtgaaaagg tatttatgat
    30901 agcccaaagc tatatgcctg cacaacaaac gcaacttctt attaatcgta aaaatccaaa
    30961 tttgagtcct tggtatagtt tagattttga aggtgatttg acaatacctc aatggacttt
    31021 taaaagagac cagttgaaaa gattttaaaa taataatttt atataataaa agaaaaatat
    31081 agatgtgaaa aaatgtaatt agattaaaac taattggata tggacaaaat taaattgata
    31141 gataaatatt aaaaattaca ctaattaatc ttactatagt ttaatattta tctaaaaagt
    31201 tttatgtaaa atataaattt tatattaaag ctaaaactaa tttgaggagg ctaatttata
    31261 ttgaagatta gaaggtattt aattttggta attataattc taattacaac aagtcagagt
    31321 tttatttctt atgggctaga gcgatttgaa gaaataacca tttttcacac taatgatata
    31381 catggaagat atgctgaagg tgatgaccat atacaaatag gaaatctagc cacattgaaa
    31441 aaagaaactc caaactcaat tttagtagat gcaggagatt gtttgcatgg acttccaata
    31501 gtaaatatgg atagagggaa aagtgcaatt gaattaatta aatgtgctgg atatgactat
    31561 attactcctg gaaatcatga ctttaattat ggaaaagata gactgtttga gttatctaaa
    31621 agtgctaatt taggagagca tactttaaaa tttttatcat caaatatttt tgaaaatgga
    31681 aaaaaagttt ttgatagtaa tgatataaaa gaaatagatg gaataaaaat aggatttttt
    31741 ggactctcaa cccaggaaac aaagagcaaa actggattta aaaatgtgga aggattagag
    31801 tttaaaaatc ctatagaaag tgcaacagaa caagtaaatg aattaaaagg aaaaggtgcc
    31861 aatgtaatag ttgcaatatg tcatattggt acaaatagtt taagtaagcc aaatagtata
    31921 gatatagcaa atgaggttca aggaattgat ttgattattg atggacacag ccacactaag
    31981 tttgaaaatg gtaaacaagt gggtgataca ttgattgtaa gtgcaggaca atatttagaa
    32041 aatattggcg agataaaact aactctggat actaatgatg tagaaaatat aaaaatagaa
    32101 aataaatcag ttagattaat tggaaaagaa gaagcattaa agtataagct tgactccgct
    32161 gtaagtaaaa aggttgatga aataaaagaa caacaagaaa aaatattaga taaagttata
    32221 ggggtaacta caaatactct agatggttca tatgaaaatg taaggacaaa agaaactaat
    32281 ttgggtaatt taataagtga cattttacta gataaaacta aggctgatat ttctttattt
    32341 aatggaggga atattcgaga tacgatagaa aaaggcaata taacaagaag agatattgta
    32401 gatgtacttc cttttaacaa tactatagtt accaaagaac ttacaggagc acagattaaa
    32461 gatgtattgg agcatggagt aaaactttac ccagaaaaaa gttcagcctt tttgcaagtg
    32521 ggaggaattt catattactt taatcctaaa caaaaagaag gcgaaaaaat aacaaatatt
    32581 caaaaagaag gaaagccatt agatttaaat aaaaagtatg tagttgcaac gaatgattat
    32641 atagcatcag gtggagatga atttccttgt ttttcaaaag agccaatact aaaggaattt
    32701 ggaaatttag agagtgcagt cattgaatat atagagtaca aaaaagaaat atcaaaaaat
    32761 gtagatggaa gaattggtat taaatctaaa gatgagttaa taaacaagaa tcatgagagt
    32821 gatttgatat taagtgaaaa tgaaaaaaat atcaatgata atgaagataa tctatctaat
    32881 aatataaaaa gctctgatag tgataaaaat aaaagcttag ataaaacaag ttctagagaa
    32941 agttctaatc taggaatatc taaaagagaa gctaaagaaa aatctcctaa aacaggagat
    33001 ttaggttttt caaatagtat aattatattt atagtttcaa gtacattgat atgtttattg
    33061 aattttaatc aaaaagagtt aaaggataaa aagtcaaaat aaaaattaat gagcaaaaac
    33121 tagtagatag taatttaatt ttagataata gtaaacagca aaggaaatta gttatataaa
    33181 tactaatttc ctttgtttta tgttttaaca tgattacttt taaataaaat tgtattttat
    33241 gaaaaataat tacactaaat ggcatagtat tatggggatt tttagttttt attcaagtaa
    33301 atgaaatcat attaacaact tctgatatat ttacaaattg ataaaaaaat gattttatta
    33361 gtatgatata atttagctag tatatatgat gatggaggat atagggatga ataatatatt
    33421 acttttggaa gatgacaaat ctttaaatag aggaattagt tttaaattaa aaaaagaagg
    33481 atacaatgtt tttagtgcat ttggtataga agaagcaaaa aatattttcg caaaagaaga
    33541 gatatgctta attatttccg atataggtct acctgatggt agtggttttg acttttgtga
    33601 ggagattaga aagaaaagtg atgtttacat aataatgttg acagctcttg atgaagaggt
    33661 ggatattgtc acaggatatg atttaggtgc agatgattat atcacaaagc cttttagctt
    33721 gatggtatta atatcaaaag tgaatgcgct tatgaaaaga gtaaatacag taaaaaatta
    33781 tactctatta gtttgtgatg atttattctt ttattatata gaaaataaat taattataag
    33841 aacagatgat aaggaagaag agataatttt aagtaagaca gagacaaaac tattaaaata
    33901 tttgatggaa aattctatgc agacattgac taaagagcaa ctgttagaat ctttatggga
    33961 tagtagtggt aattttgtag atgataatac gattgcagta aatataagaa gacttagaca
    34021 aaaggttgaa aaaaatccat ctgaacctaa atatataaaa acagtaagag gggttggata
    34081 tatgtgggga gaaaggagta taaaaaaatg ctaaaaaata tgaacctcaa agatggttta
    34141 agtgtgaaag atgattatat aatgagaaga gttattgtat attctgtttt atctgtacta
    34201 atagtaacca ttgtatttat actttttgaa tatagcagat tacataaatt gtatgaacat
    34261 caaataaaaa atactcaaaa tattgctgga atattaattg aaaaatatcc taatgatgaa
    34321 atagatatag taaattctat ttataattct gattatagta aggtgtctac tggagaaaag
    34381 gcatttaaga aatttggcta tggattggaa aataaaatgt caaatgataa aaattttagc
    34441 ttatacttaa aatctttttt taaggaatca ttttttattt ttttaatgat gtttatagta
    34501 gttataggta taatatatta ttttataaga tacataaata aaagattgag taaaatttat
    34561 tttatagtag ataagatgtc acaagaaaac tacttgaagg acactactga gtcagaagaa
    34621 actggttatc tagtagaata taatgaatac tttaaagaag gcatatttag taagataaat
    34681 aactgtctat atgagcttaa tagaagttta aagataaagt ttattaaatt agaaaaggaa
    34741 aaagaaagtg taaaatcatt agtaacagat atttcccatc aattaaaaac tcctttagca
    34801 tccttaaaat tatacaatac attacttata gaagaagaat tagaagaaga tgaagccaac
    34861 gagttcttat taaccaataa ggcttctata aataaattag aaaatttaat aaattcttta
    34921 gtgaacatat caaggttaga aattagcatg ataaacataa aaaaagaaga taatgatata
    34981 aaaaatacta ttctaaatgc agtaaaaagt gttaccccta aggctaagag caaaagtata
    35041 acattaaatt taaatgaatt taacagtaga attataccac atgataaaaa gtggactgag
    35101 gagagcattt ttaatgtatt agacaatgct gtaaagtata cacaggtaaa tggagaaata
    35161 aatatatctg tagaagaaac tgctaatttc tttaaaatta taatagagga taatggcata
    35221 gggattgaaa aaagtgagtt taataatata tttaaacgtt tttatagagg ttctgctgat
    35281 gaaattgaat ctgtagaagg tagtggggta ggattgtatt taagtagaaa gattttagaa
    35341 gaacaaggtg gaaatattat agtttcttca aaagtaggaa tagggagcaa gttttctttg
    35401 tttcttacaa ctatgtaata ttaaatgaaa ggaaaatgta agcctctaca tttattatat
    35461 aagtataaac agctccagaa ataaaaaatt aataaaatat ggagtaatat aacttagaat
    35521 gagaggataa ttttatgaaa atactatata ctgaaaattt atcaaagcat tatggaaaag
    35581 gagaaagctt agtaagagct ttggacaatg ttgacttaga aataaatgaa ggtgaatttg
    35641 ttgcaataat aggaaaatca ggttctggaa aaagtacact tttgcatatg attggaggtc
    35701 ttgatatacc tacatctggg aaggtgtata tagataataa aaatatattt acattaaaag
    35761 aagaagaact tgctgtattt agaagaagaa aaatagggtt tatatttcaa agctataatt
    35821 taattccatc tcttaatgta tgggaaaatg tagtgttgcc aatcggttta gatggtagag
    35881 aagtagatga atcttttata aaagaacttt taaaatcatt aggacttgaa aataaacacg
    35941 acgttttacc aaatacatta tcaggtggtc aacaacaacg ggtggcaata gcaagagcat
    36001 tagctacaag accagctata atacttgctg atgaaccaac tggaaaccta gattctaaaa
    36061 cgtctgatga ggttatgagt atattaaaat ctatgtctaa gaagtacagt caaactctag
    36121 ttatgataac tcatgatgac agtatagccc aaatggctga tagagttata ttcatagaag
    36181 atggcagggt gtcaaaggtt ggtgataaaa atgattaata ctactaaaat agcaaaaagt
    36241 aatttaaagc aaaataaatc aaaaagtatt ctaataataa ttactataat actttcaacc
    36301 ttaatgcttt cttctattgg aatttatatt gttgatgcag gagcatatca aaaagaaaac
    36361 actataaagt attcaggtaa ttatcaaggt atattagcaa atgtagatga aaaacaggct
    36421 gatatattga gtaatcatgc agatgttgaa ttaactggtg aaatgaatgg agtaggtgtt
    36481 gaaaaactgg aagatgattc aaatatttct ctagcataca tgaatgaaga tgctttgaaa
    36541 ttaaatagtt ttgaatttat caaaggaaaa ttgccatcaa aagagaatga aattgtgtta
    36601 gatagtggag ctttaaaggc acttggttat aaaaataaag atttaggtga aaagataaaa
    36661 atatcttatg atgactataa aagtgataaa aaaattgaaa aagagttcat tataagtgga
    36721 atcttaaaaa ctagcgaaat atctgaagca ggtaaatatt attatgctat tatatcagaa
    36781 tcatatatga gaaatactag agatatgtca caagaagatt ttaatatata tgtgaaatta
    36841 aatgataaaa gtaatttatc gatagaacaa gcaaaatcaa aattaaataa gattgctaat
    36901 gatgtaggac tggacactat taatgtagct gtaaatgaaa actatataaa tgctttaaaa
    36961 ccagatatgg aaacaattat gggtggagta tttgtagggt tagtaattgt attgtctagt
    37021 attttagtaa tatacaatat attttatatt tcaatagtta caaaggttca agaatttgga
    37081 aaacttagag caattggtgc aactaagaaa caaataaaaa atatagtgtt taaagaaggg
    37141 ttcatattag ccggaatagc aataccaata ggaataattt taggctatgt acttgcaaat
    37201 attataataa aatcatttat ggatatagat gtaaaatcat cacgattacc agttatatta
    37261 tcagttgtgg taataagctt tatatctgtt gttttatcac ttttaaaacc aatgaaagta
    37321 gcatcaaaag tatccattgt tgacgcagta agatatacag gaaataaaat aagtaataag
    37381 agtaagagaa aaggatataa aaatataaat ctaaagagat tatcacatgc caatttagaa
    37441 agaaataaaa aacgtactta catgacattg gcatcactta tacttagtgg aactatattt
    37501 ataacagtat caactgcctt agaaagtttt gatgctgaga aaatggcaag agaacacttt
    37561 ccttatgata tagaagttag actatctggt tatgaaatga acagtgataa aaatcctaaa
    37621 gataatttga atattttaca aatggataat cctctaagta aagatttttt taatcaaata
    37681 aagaacatgg aaggaattaa aagaatagaa tctgcaagaa gtgtaaaaat tggtatggag
    37741 gactatgatg ttgaatttaa gtatgacctt ttacaaagta taaatgaaaa tgatgtaaaa
    37801 tcattaaaca agaatataat agatggaaaa ataaatcttg aaagacttca aactggagat
    37861 gaaatagtaa ttactcatgt tgatacagca aaagaaatgg gagtaaaagc aggagataaa
    37921 ataagattaa ctttatatga tggagataaa aagataaaaa aagaatttaa agttcaagca
    37981 atagctatgg gggttccaag ttttggtata gggaaagatt ttatagatag aactttgaaa
    38041 tacgattcaa ctagtagctt gggaatatat acagaagaag gaaagtatca agaaatcaaa
    38101 gatagtatca agaagattgc taaaaataat ggatttttag agactgattt tatagatagt
    38161 agaattgagt caaataaggc tactatttca tttataaaga taatgggata cacgctaaca
    38221 ggcataatag gtgtgattgg atttatgaat cttgtcaaca caatgataac tagcatagtt
    38281 actagaaaaa aagaacttgg tatgcttcaa gctattggtc ttactaataa acaattagtg
    38341 aaaatgctta attcagaggc aatatattac acttctggaa tgatgatagg aagtattcta
    38401 tttggaggta tattagggta tattgctgtt atatttctaa aaaagactgg attatcttat
    38461 gcaacatatt ctttgccaat agtaccaatc ttgttgatga ttgtgtgtat tcttatagct
    38521 caatttataa caacttattt gataggacga agttttaata aagaatctct tattgataga
    38581 gtaagataca gtgaataaaa tgcaaatatt tatggttagt ttaactagca aaaaacatat
    38641 aaaaaaatat agttaaagag attcataatt aaataggtta aagcatagta cagagcaaac
    38701 aatttaagca gatgtttgct ctgtatttta ttatgtaatc aataatatat ctaatatact
    38761 gatattattc atgttagaaa atgtaccggt acaattctaa aaaagtataa aattgtacca
    38821 tataaaatga aaattttgta taataatcgt atagaaaata aataggagct gatatatatg
    38881 aataagaaga ttaattatgc accaggacca actgaaacta gggaaaatgt aagattagca
    38941 agagcagaaa aaacaacaaa tccagatata gatattgatt ttgttgaatt ttataaaaag
    39001 acatgcgaga agtttggtaa tataattgga acaaaaaacg atgtttacat attgagtggt
    39061 gaaggaatat taggtcttga agcagcatgt gcatcactta cagaaaaagg agatagagta
    39121 cttgtaattg ataatggtat atatggagaa ggttttaaag attttgtaac aatgtatggt
    39181 ggagagtatg tgcttttttc aagtgaatat actaaaagta tagatattga tgaattgaaa
    39241 gcatttttag atgaggatag taattttaaa tatgcaacag tagtgcattg tgatactcca
    39301 actggcgtat taaatgatgt aagcaaaata tgtccattac ttaaagaata tggaatatta
    39361 actgtagttg attctgtagc aggtatggta ggagaaagat taagtgtaga tgaatctaaa
    39421 atagatataa tattaggtgg ttcacaaaaa gctatttctg caccagcagg acttactata
    39481 gtcggaataa gtcaagatgc aaaaaattgt ataaaaaata gaaaaactga tgtaataggt
    39541 ttttattgta atttaaatat atgggaaggc tattatgaaa aaaaatactt cccttataca
    39601 atgccaataa gtgacataat ggggcttgat aaagcacttg ataatatatt agaagaagga
    39661 atagaaaatg tattaaatag acatgaaaag atagcttata gtgttagaaa atctattgag
    39721 gaatatggtc tagaactatt tttagaagaa ggatattcta atacagtcac agctataaaa
    39781 gttcctgaaa gtataggagc tttagaactt actgattata tgcttaaaaa ttataataca
    39841 cttgttgcaa catcactaaa tcaatatatg gatactattt taagaatagg tcatatggga
    39901 gagaatgcaa atctaaataa aatagaacat gtactaaatg ttttggataa aagtttaagt
    39961 gcactaggat tcaaaggaaa tggaaatttg ttaaatttat ttaataagta ttatttttaa
    40021 tagatagggg gatatagtat ggaagttaca gctaagcaaa aatttacaac gaaagacatg
    40081 gtggaaacct cactattaat agcattagta tttattgcaa ctaaatttat aaacataaga
    40141 ttaccaatat ctataaatgg tgggcttgtg catttaggaa ctgcaatgtt gttcatatca
    40201 gcaatagtat ttggaagtaa aaaaggtgct ttatcaggag ctattggtat gtcacttttt
    40261 gatttgatat ctgggtggac tttatgggca ccgtttacct ttatagttag aggagttatg
    40321 ggttatttac ttggaaagat tgcatgggca aatggtaata atgggaataa ttttttaatc
    40381 aatgtaatag gaatatgtgt ctcatcaatt tggatgctat ttggatatta tgtaacagaa
    40441 gttatacttt atggtaattt tatagtacct cttacttcaa taccaggtaa tttaatgcaa
    40501 gtattgatag gtcttataat agcgcttcct atttctaagg tattgaaaaa atgtataaaa
    40561 taaatataaa gtgaactaaa atttatgata gcataaatta tactgtataa tattaggata
    40621 gagtacataa ttaaaaaatt ttagtataga ggttagttca caatgttatc agttacaaga
    40681 tattcaatca aaaatgtccg tttaaagccg tgggtaaagt ttatttggta ttttgagaca
    40741 aaatcaaata tactgttaaa taataagtta cttccgacag acagtataga tattattctc
    40801 aatctttctg atgttatgga atataaaatt gaaaatcaag actatactgc tagtaatatg
    40861 cattttaatg gaataaggga taaacatgga tttattattc aacatggaaa tatacgtgtt
    40921 atagggattt ctttttatcc atttggtctc tatccatttt taaagatacc aatttcagag
    40981 tttaacagac aaattgttga cctagaagca gtatcacagc tatttgcaaa aaaattggaa
    41041 gagtcattaa atccaacaca atcagtagaa aaaattgttt tatatttaga agaagtatta
    41101 ttatccatat tagaagaaga tttaatttca aataaatatg taaagcttct aaattcattt
    41161 atatatgtta atagatatag caatataaaa gcattttgtg atgatacaaa tataaatatt
    41221 aaaacattgg aaagaatctg tttaaaatat acagggtata cacctaagat tttaaagaga
    41281 atttacagat ttaaaatggc aagcaatcaa cttatttata attataaaga tgatgaactt
    41341 tttgatttta tttatgaaaa tgagtactat gaccaagcac actttataaa agaatttaaa
    41401 aaattttcag ggacgtcacc aattaaattt agtggtgaga ataagacgat taaagaaaat
    41461 ataaagtaca gctatttata attatactgt cgatttttta caatacctcc aatttatttt
    41521 ttgatatgat ataggaaaat aaattggagg ttttacatat gaaaattatt acagaaatag
    41581 ttgagtttaa tgtggaagaa aatttatcaa aagaagagtt tatagaaatt gtaaatgact
    41641 tagaattaaa atttcattct attcaatctg gttttattga tactgagctt ttatatgatg
    41701 aaaagtctca aaagtggatt atgatacaac attgggaatc tatagaagac ctcaagagtg
    41761 cttctagtaa aatgtttaaa gattcttcaa ctgagaaatt tagaaaagct ttaaagaatc
    41821 aaactgttaa gatgaatata ataccacaaa ttaaaagttg gaaattatag aactgaatgt
    41881 aaaagatagt taaatagtat caaactaaat tagccaaata acattttgta tttaaattgt
    41941 tatttggctt ttagtgttta ttttaatatg agtgttatat actagaaaaa ccaacaaact
    42001 gttagagtgt ggattatcat aatgtcatat tcgggagcta aaaggcttaa tactgatttg
    42061 ccaattggtc aatccatgca tgtccatctg gatatgctgt tattaaagca tttacaagcc
    42121 aatccctttg ctcttgtgat aatagtggga ctgttacatc gaagtctttt tgataaccaa
    42181 gcctatctat gtatattgat ttgcaaaaca aaaccacttc gggagccata tatgggatat
    42241 tatctgatgt tatcagtctg gccttatcca tatctcttat aattctttga ttttgggaaa
    42301 aaagaaattt tccatctttc tgtggattat atatcaattc aataaaatcg aactgtaact
    42361 gttcgtcaca ctgtatattc attttatagt tatcatcctc gacttttttt aattcaataa
    42421 atgagcaatc aggcttaatc gcaaaaacac aaatttgctc tatatgttgc ttgttaaggt
    42481 cgataatagg tcgtaataaa taattaccca agggctcata tatatcccat ccttgattaa
    42541 gcatatactg aatgctcttt tctttatctg tgtcagaaat ccatatgtca atatcactgt
    42601 gtgggcgaat atgcatattc gcaaataatt ctagggcata tccaccacat attgaatatg
    42661 aaaagccaca atcattaaaa aagtttacac aatctttaat tagtttgttc atagcaacct
    42721 ccttttgcat gtactgaaat aaattaaatt tttaaaaata ccaactaaat gtttgttttg
    42781 tgtaaatata aaactgccga tattaaggca gttttatatt tactatttag aaaatttatt
    42841 aacaaatttt tgatattcac acattatctt atcattcata aaagaaatac ccataaaagc
    42901 agcttgctca ccaaacaaag taaaacgttc tatcatctgt tgtggattac ctggataaac
    42961 tgttggaata caccatgtgt ttagaagaaa accaaacatt tgagcagcaa tctctggatt
    43021 tttaacttcc atagaattat cttgatttcc ttcttcaatc aatgctttta tccaaggcac
    43081 aatatcattt ataatctcat caaactgcgt agctaaatac ttaggattac gcaatagtga
    43141 tgatgacatt aaaaaaatgt cttgtttatt gtgttgttca taagcatgaa gcaacctata
    43201 ttgtaatttt tctaatgcgg tctttccttc atcatttttt acctcatacc cataaaatcc
    43261 atcattatac aaccgacttg tgattgcctt aattatttcg tcttttgatt taaagtgatg
    43321 ataaatagca cctcttgaaa ggccatctaa attatctaca atgtcttgaa tcgttgtctg
    43381 ctcataccct ttttctaaga ataacttatg tgaaatatca agtatttttt gtatagtatt
    43441 atcaggttct ttttttcttc ccaaaaagct cactcctcaa aacaatcatt tggtatgttt
    43501 atcttagcaa atacataatg atttgtcaat gtgtcttttt agcatgttga acaggtatat
    43561 tgccatgctt tcattatttt atttttttgc cttatactgg actatatttg cagtttccta
    43621 atatatttgg ctcatattta aataccatta tctaaaagca ctattgctgt acgagaggca
    43681 ataggcactt tagaagaagg aggagttgag ctaaagtata catcataatc tcttaagttt
    43741 tctttttttg tgtttcctcc aaactctacc atatcagtat ttaatatcgt tttgtagttt
    43801 gtattttgaa actttgaaat atcaactgat tgtattagat tagggtggaa attaaatata
    43861 aataaatatt tgcccttact gtacaccaaa atctttttat cattatccat atgtataagt
    43921 tcatgactat tttggataaa aatatcactg tattttgtaa gttcaagcat agatttatca
    43981 aaacttgata attgtttata ctttaagttg ttatcttctg aaaggctcca ttgtcttctg
    44041 gcatacttat agctccaatt gttgccttct cgtggaaaat caatccactc aggatgtcca
    44101 aattcatttc ccataaaatt tagatatcct tcacctgcta gagaaaaagt tattaattta
    44161 attaatttaa taagagacat tgctctattg ataacatggt tatttgagtt tatttccata
    44221 ttccagtaca tttccttatc agctagccaa aaaataattg ttttatcgcc aactaatgct
    44281 tggtcatggg attcacaata tcctatattt ttttctccag gtcgtctagt agtaagttca
    44341 taccacattt tgcccaaatc ccagtcttca tcagataagt tagagatagt ttttatccaa
    44401 aaatcaggta ctcccattgc gagtctatag tcaaatccaa ttccgccgtc ttttataggg
    44461 atacacatac caggcattcc actcatatct tctgcaatac ttatcgaatt aggtttaatt
    44521 tcttttatta attcatttgc aaattggaga tatgttatag cttcaatatc tgtattcata
    44581 ctaaaatatt tttcataact atcaaatgaa actccaagac catggttatg atataacatt
    44641 gaagttacac catcaaacct aaagccatca aaatggtact catttaacca aaattttata
    44701 tttgaaagca aaaaatgtat tacttcaggt ttaccgtagt taaaaagttt tgtaccccaa
    44761 gcaggatgat ttccctttga acctgagtgg aaaaattgat gttcacttcc atcaaactca
    44821 tttattcctt ctaaagtatt ctttacagca tgagaatgaa ctaaatccaa taaaacagca
    44881 atccccattg aatgagcagt gtttatgagg ttttttaagt cttcaggagt accaaaccta
    44941 gatgagattg cataaaagtt tgaaacttgg tatccaaaag atgcataata tggatgttcc
    45001 ataatagcca taagctgaat ggtattgtat ccagcttttt ttatcttagg aagtattttt
    45061 tcagtaaatt cattataagt tccaatagat tcgctttcag tagacatacc tatatgacat
    45121 tcatagatta gtggagaagt tatgtttttt aagtcaaaat cattgtcagt ccagataaat
    45181 ggagtttttg gttgccatat ttgtccatta aatccaccaa aatctttttg aattactctt
    45241 tttatatata atggtatacg gtcaaatgtt tttccatttg cagtaacttg tacctttacc
    45301 tctgatttgt gaggtaagct attttgacca ggaataaata tttcccaatt accagaccca
    45361 atttttctta gaggatgaga ttttctattc caattattaa agtcaccaat tagtgacaaa
    45421 ctatctgcat ttggagccca ttccctatag aagaatccat catttgtctg atgaaatcca
    45481 tagtaaagat gagcatttgc aaaagaacta aaatctttat agtttttcaa tgtttttgat
    45541 ttcatatcct cataatgttc cattcgcttt tttatatcag attcaaatgg ttgtaggtat
    45601 acatcaatat ctaaaatttt aaatcttttt tgacttttca ttgtatttct cctccataat
    45661 aaatattaca tctaaaaaat aatatttatc tcatactatc ttctctgtat aattgatata
    45721 tattatataa tcctaataaa aagcttaagc caccaatgat taatttcata atttacctcc
    45781 ctagtattta tacttatata tcgtattcat caaaatttaa aagtttgttt aaaactcttt
    45841 taataatatc ataatcaaag cctttatagg aaagatgttg agaaattttt tgatatattt
    45901 tctttttatc ttctttcttt actctttcat accttttttt tgctagatac atagcatttt
    45961 caaattcagt gtccttgtct aattcagata ctgcttcatt gattgtactt ctattgatac
    46021 ctttattata taaattctgc ttaattctgt ttttgccaca tttatttagg ttaacatttg
    46081 tgttaacaat tttttgagct aatacactgt catttacgag attataactt ttaagaaaat
    46141 ctatgaccct ctcaattata ttttcatcaa attctgatga tagtttttct tttaattttt
    46201 tttcagattg gtcagcatga gataaaatat ttaatgcttt atttttagct tttatataca
    46261 tttcatcatc taaaataggt tttaattctt cttcatttat ttcattccct ttttttaaat
    46321 taaatgtata aacaagttct ttaaatatag caataaaaaa tttatcatct acataaatat
    46381 taactctatc atcatttctt ttttgttgtt ctatttttgt aattataccc ataagtcctc
    46441 cttttttata tgtaattata ttaacaaaag caactaataa aatttaacaa aaaacttata
    46501 tttgtatgtt atattttatc aaaattaaga caaaatagta aaaagttatt tttattaaaa
    46561 agtaaagcag aatcatgtta aataattatt ttttaaagtg ttttttagca tataaatata
    46621 attatatacc caaaaaaaca aattataagc tttaagaaat aaaaaataat ggtacaatag
    46681 tattataaca ttaaaaggag acaaaatatg agaagtgaaa atctagttaa gatggcagag
    46741 ttgaaaaata ctgaaaaact agagaaattt aatcgccata aaggcaaaat caaaataggt
    46801 atattgggag gtacttttga ccctatacac tatgctcatt tagcaacagc agaatttatt
    46861 agagacaagt atgacattga caaaataatc tttataccat caggaaatcc accacataaa
    46921 ttatgtatca ctacagataa gtatgataga tataatatga cacttctagc aactgaaagc
    46981 aacgaagatt ttttagtttc aaaagtagaa atcgaaagaa agaaaaggac ttatacaata
    47041 gatacattaa aatacttaaa gaaaaaatac aaaaatgcag acatttattt tataacaggt
    47101 gcagatgcaa tttgtagtgt tgaagagtgg aaagatgtaa agaaaaactt tgagttagct
    47161 acttttatag ccgcaactag accaggaatt agtttgttaa ggtcacaaga gactatagaa
    47221 aaactaacta agaagtataa tgctgacatt ataacagttt atgttccatc actggatata
    47281 tcgtccacat atataaggga acagctaaat gaaggcaagt caatacgtta cttagtaccc
    47341 gaaaatgtag aaaattattt atatgaaaat aaactatatc aatatggaga tgattaaatg
    47401 aacttagaaa atattaacca aagactaaat caaatgctac cagtggggag gcttagccat
    47461 tcaaaaaatg ttgcaaaatg tgctgaaaaa ttatgtgaga tttatggatg tgacaaagaa
    47521 aaagcttact tagcaggaat gattcatgat tgtgcaaagt atttaagtga caaggaaata
    47581 gaggattatg taaataagta tgaaatatat ttagacccaa tagaggatgg aaatcgttcc
    47641 ttgtctcata gtgtaattgg ggcgtacata tgcgagtatg aatttgaagt agaagatgaa
    47701 gatattataa atgcaatcaa atatcataca acagggagag aggacatgtc tcttttagaa
    47761 aaaataattt acatagctga tttaattgaa gaaggaagaa aattcccagt tgtagataca
    47821 ttaagagaat tagcatatgg agggaagctt gatgaagcac ttttaacttc ttttaataat
    47881 actttaatgt ttgtcatcaa taaaaaagaa gagatacatc caagaacagt tatggcaaga
    47941 aactatctaa ttaaagaaaa gttattataa aaaagctata taaaggcaaa catattaata
    48001 aatttaaatt tgactatgtt acaaaatcgt attaatatgt tacaataact aagtgtattt
    48061 taattaggaa agtgaggaat ttttatatga cagtggaaca aatgacaaaa atagcatatg
    48121 atgccataga agataaacta ggtcaagata cagttataat aaatatagga aaagtttcta
    48181 gtttgtgtga ttattttatt attactacag catcttctca gagacaggta aaagctattg
    48241 ctgacaatgt agaagatgaa ttagcaaaac taggattaga gccaagaggt aaagaaggac
    48301 aaggaactca aacttgggta ctccttgatt atggagacat aatggttcat gtatttaatg
    48361 aggaaaatag agggttttat aatttagaaa aattatggaa agatgcacct tatatagata
    48421 ttgacacatt agcataaaca tgataatata tgttattata attagaaaat ttcaaaaatt
    48481 atattaaatt gaatagacta gagcagtaaa agattatatt ttttcagaga gttagtggaa
    48541 ggtgagaact aatattatat aactttgaac ttactagatt aaaatctatt ctccatggct
    48601 ggattaaaag ctatctaaga gagtctatat atgtagtagg ctaattaggg tggtaccgcg
    48661 aagatttatc ctcgtcccta aacgtaagtt tagtgacgag gattttttat tttcaaagaa
    48721 ttatttggaa attataagta aataatacta aataaggtag ttatacacaa ttaaaaatag
    48781 gaggcaaaat atgagcgtat ataattttaa agaagtagaa tcgaaatggc aaaaaatttg
    48841 gaaagataat aatcagtata aaatggatac tgcacaaact gaaaaaccta attattacac
    48901 attagaaatg ttcccttatc catctggaaa aatacacatg ggacatgtta gaaattattc
    48961 tataggtgat gttgtcgcaa gattcaaaaa aatggaagga tataatgtac ttcatccaat
    49021 gggatgggat tcttttggat taccagctga aaatgcagct ataaaacatg gaatacatcc
    49081 acataaatgg acaatggaaa atatagaaga aatgaaagag caattaaatt tattaggtat
    49141 tagttatgat tgggataaag aagttgcaac ttcaacgcca gaatactata gatttacaca
    49201 agaaatattt ttgaagtttt tagagcatgg gcttgcatat aagaaaaaat catatgttaa
    49261 ctggtgtcct tcttgtgaga ctgttcttgc aaatgagcaa gttgttcaag gagcatgtga
    49321 gagatgcaaa gcaacagtat taaagaaaga tttagaacaa tggtatttta agactactga
    49381 gtttgcagaa gaattactta atgacttaga tacattagat ggatggccag aaaaggttaa
    49441 aacaatgcaa aaaaattgga taggtaaaag tacaggagca gatttagttt ttgatataga
    49501 tggaactgat aaatctatga cagtatttac aactagacca gacacaactt atggagttac
    49561 ttatatggta cttgctccag agcatgaatt agttaaagaa ttggttgcag ggactgaata
    49621 tgaagcagat gttgaagctt ttgttcaaaa aatgcatact atgacagaaa tagaaagaac
    49681 agcagctgat gtagaaaaag aaggaatgtt tataggtaga tatgttataa atcctttaaa
    49741 tggcaagaaa gttcctctat ggatagctaa ctatgtatta gttgagtatg gaacaggtgc
    49801 tataatggca gttccagcac atgatgaaag agatagagat tttgctgaaa aatataattt
    49861 agacataatt gatgtaataa cagaagataa caaaatgata aattcagaag aatttaatgg
    49921 attagatgct tcagaaggat ttgaaggaat aatcgataag ttagaaaaag aaggtagagg
    49981 aaagagaaca attaattata gacttagaga ctggttagtt tcaagacaaa gatactgggg
    50041 ttgtcctata cctgtggttt attgtgatga atgtggaata gttccagtta aaaaggaaga
    50101 tttaccagtt cttttaccaa cagatgttga atttactgga aaaggggaat ctccacttac
    50161 gacttcaaag caatttatgt caacaacttg tcctcactgt ggaaaaccag ctagaaggga
    50221 agttgatact atggatacat tcgttgattc ttcttggtat ttcttaagat atgtggatag
    50281 caataatgaa aatgaaccat ttagtaagga attagttaat agatggcatc cagtagacca
    50341 atatataggt ggagtagaac atgctataat gcatttactt tatgcaagat ggtttgtaaa
    50401 ggcatttaag agtatgggaa tggtagactt caatgagcca tttaaaaact tacttactca
    50461 aggaatggta cttatggatg gttctaaaat gagtaagtct aaagggaata cagtatctcc
    50521 tatggatata atagatgagt atggagcaga tactgcaaga cttttcgtat tatttgcagc
    50581 accaccagaa agagatttgg actggtcaga gcaaggtgtt gatggatgct ttagattctt
    50641 aaatagggtt tatagattgg ttgacgagtt agcagatgtc gttaaaaagg atgtagagtt
    50701 tggagaatta aattcccaag ataaggatat gagatatact atacactcta cacttaaaaa
    50761 agttactgct gatttaagtg aaaaattcgg atttaatact gctatatcag ctcttatgga
    50821 attaataaat gatatgtata aatataaaga gttagataat ataaacgaag cagttataaa
    50881 agaaggtgta caaactatag taactataat tgcaccattt gcccctcatt taggtgaaga
    50941 actatggact atgataggta aagaaggaag tgtatttgac ataaactggc ctaaatatga
    51001 cgaaaaagct ttagtaaaag atgaaataga agttgttgta caagttaatg gaaaagttag
    51061 aggaaaatta actgttaact caaatatatc taaagatgag atggaaaagg tagctttaga
    51121 agatgaaaaa ataaaaggat tagttgaagg taaaactata gttaaagttg tagctgtacc
    51181 taaaaaacta gtaaatatag ttgttaaata actcaaaaat atgaacaaaa atataaattt
    51241 ataaaataaa cgaggctgtc tcaaaataga gataagcctt ttttcattgt tgaaattata
    51301 aaattgttga aattataaaa atagatttgg ttttatacaa aaaaatgatt aaagaactaa
    51361 aaagttcatt cttatatacc ctaattgttg ttttaaatat tattataatt aatgttaaat
    51421 cgagttaatg tattaagtgg tatgtaaatc attcagtttc caaagtgttg ttcatcaagg
    51481 aactgctcta agcttaaacg aaaaaaatat aattattgta ttttgaaatt tttttctata
    51541 taatgaaaac aggcaaaaaa ggtgcatatc tatgctttat ctgaaacacc cagacaacga
    51601 gattcagatt catataatag ctaccatgtt tctaaaaagg aacttgcaaa aaaaataggc
    51661 gtttcaactt cccagttgat taaaattatc accaggaaaa caaggatggt cagcagtagt
    51721 attctaatgg gtgttgctga tatatccaag ttgagattgt ttgaggatgt tatcaaataa
    51781 cttgtgatag gtgcagtaga tgtggagatt cctgatacgg atttattctc agggtatggt
    51841 tgcttcggga gatatggagc gtaaccagtt tattgatatg gtaaccattt ttttgtctaa
    51901 tttgatgaaa gaatcccaag aataccatac agaagtaaga caggaattaa ctggtggtat
    51961 aaactgataa aggcaaattt aaaaattttg ataggatgat tttaagattt atcttctatt
    52021 ttgtgtttta gaaataattg aaatgttatg aggaggtatg tacaaatgga gactgtaaca
    52081 aataatcaat caggtgtaga aattcaactc agtctgtttg aagctatcaa agatttattg
    52141 ttaattaatc ctattttctt gttgggaatt atcttgattg gtattttctt agttgtaata
    52201 ttcaagaaaa atagtgatat tccaaaggta aaaacatcta ttttatcact ggcaatgtat
    52261 tactacttat gtttaacact atcgcatatt gttggtatac ctacattcgg tgaatatata
    52321 agactgtcac agttagggga aacgttcttt aatccaaaca taaatcttat tccttttagt
    52381 gatggattta gtttgagttt tattttgaat attttacttt ttgtcccatt aggtttttta
    52441 tgtccgctta taagcaaaac ctttgaacgt ttgagaaata cattcttgat tggttttgga
    52501 ttatctcttt ttatcgaaac tattcagctt tttacgttac acagagcaac cgatatagac
    52561 gacttactca cgaatgtaat tggaacaata ataggctatt tctgcttcaa actgattgct
    52621 aaattgcgaa ttgtaaaact acattcagat gatgactcta tggaacagga ttacacagta
    52681 tgtattccga ttgccattat aatagttgcc cttatattag gattttttag ttaagttcca
    52741 aatagctaat ctgggaatcc agacaatata acagatagta tgttaccttt tacaggagca
    52801 tactgtctgt tatgttgtca acatctcatt ttatggtttg tgtgtagttt cctgtaaact
    52861 gaactaaata attaaataga agctattaga attaataata acagatagat attaataagc
    52921 ataaacaaat agtaaaatgt gtagatatag acgtttttaa gtgaaactat tataaaataa
    52981 ttgactgata atcaactgac aaaacctatt tttttaggtt taactgacag tcagttttta
    53041 ctatttaaat agaagttcgc ttagattgtc ggaagcttct ttgtccattt ctttaagtac
    53101 atgagaatat ctattcatag ttatttttat atctgtatga cccaatcttt cagaaacaac
    53161 tttcatatta gttcccccta aaagcagcag agtggcactt gtatatctta aatcatgtat
    53221 tctaatcttt tttaaattat tttttttgta aaattgtgaa acgtttttct tagtgaagaa
    53281 ggtagaaacg gatttaaatt ggtgtttaga caaactaagt tatttctgtt ttccaggata
    53341 ccttacaatt ttaatttgct ttgtcttagt ttttcaattt ttcaattaat tcttttggag
    53401 ctgataaagc tctttttgat tttggtgttt ttggctcttt aaaagatatt ttgccattag
    53461 aatagacaag aatttgatta acatttatca gattgttatc taagtcaaca tctgaccacc
    53521 taagcccagt agcttctcac attcttaaac ctagaagtaa cattaaaaga ataggtattt
    53581 ctatattatt accaagtaac ttttctatta gcaataatac ttcttctcta ttttatattt
    53641 catttgtaga atatttttct ttagaaggaa gttttataaa atcacatgaa ttttcttgta
    53701 tttctcttaa tctatacgct tcccttatta cagaacttag aaagctatat cttactttag
    53761 cactagtaaa ggtagaatta ttaaagttat aatttacaaa agcttgtact gaactaggat
    53821 ttacttctgt gagttttaga ttcttaaaaa aggttctatg taatttttaa cccaagattt
    53881 tgtatttgta acagtatatg gagaccaatt atttttattc atgtctatat atttataaca
    53941 tctttctaca aatgtcatac cttttggagc aacaaactta ttattattta tagtagactt
    54001 tatttcaatt aaatgttttt cagcatcctt tttcttttta tagcttccat aacttttttg
    54061 tttctttttg ccagtttctt attccacata tacatagaat ttttcccatc tttttcttat
    54121 aaaagctgat ttgatattca tatatgtacg ctccctttat aagttagttt tataaaagaa
    54181 cagttggact gctctaatta tgtcattatc tttttaattc tttacagtta cattataaca
    54241 cgtatttgta tacacttaaa ttaacaggaa gataaatatc gttaaaggag gaaaagaaaa
    54301 tttaatgtag aattatgtaa gatatatagt atttcatata taaaaggggg agatattgtg
    54361 aaattaaaga aattattatc actagggtta gccgtgtcaa tgtttgctgc tggtcctata
    54421 tcagctaatg cacttgatac aatttatact attcaaggaa aagataaata tgaaacggct
    54481 gctcttatag ctgatgaaca aaattatact acagctatat taattaatgc agacagcaca
    54541 atggctgatg gattaagtgc gagtggactt gcaggagcaa caaatgcacc aattttttta
    54601 actaagaaaa ataacatacc taatgcaact cttaaaagag tagagaaagc taaaaaggta
    54661 tacataatag gtggagaaag ttctattgat aaagcaacag aaactcttct aaaagataaa
    54721 ggaatagagg ctaaaagact tcaaggtagt gatagaatta agacaagcta taatgttgca
    54781 aaagaaataa actcaattaa caaagtaaat aaagttatat tgactaatgc ttttaaagga
    54841 gagcctgatg caatgagtgc tgcacctgta gcagttagag ataaagcagc tatagtatta
    54901 actgatggaa aaagtgttcc atttaataca actggtgtag aaagttatgt aataggtggt
    54961 acttcatcaa tgagtgacaa actagttaat gatactaatt caactagatt aggtggagta
    55021 gatagatatg atactaataa aaaaatagta aataagttct ataatggcgc aaaagaattt
    55081 tatatagcta gtggtactga tttagtttat gctttagttg cttcaccaat ggctaaaaat
    55141 aatcctattg tactagttga tattggaagt aataaagata tacttaaaaa tgctgcaaaa
    55201 gtgactggga taggaaatct aagtgataaa attatgctag agtgtgaaaa taccatcaat
    55261 aatattagtc ttccgtcttc aaataaaaat ataatatgtg gattaaagat tgattttgat
    55321 tcaaagcaca taatttggca agataaagga agttatcttg aatgttatga tgataacctt
    55381 gaatattata ctgaaaaata taaagaccca acagttccta taaatataga aaatacaagt
    55441 acacaaaatt tagatgtaac aaaattaaaa gtttatggag aagctaattc aacattatat
    55501 atggggcaat atgctaaaat aaaaatggat tttaatagtt tcggtgtatt aaagccagga
    55561 gaaaaatgta ctatttattt agatataatt tcattagatc catcaagaag catgacatta
    55621 acattttctt atgtaggtga taaagaaaat tataaatatg aacaaggatt ggcaagtaga
    55681 atattatttt ataacccaga tggagaagat ttttttgaat ataaataact agtgggtatg
    55741 taaattaata agggttttat aagtttaatt tttttatttt cgttttatat taactatgtg
    55801 gtatgtaaat gtctcgatat tgctcatatg ttgagttatt atatttagtt tcatatgaac
    55861 tatatggatt ttaaaattaa aaatacttaa aaacatttac tttaatagta ggtgtttttt
    55921 tgattaaaag aatattatta taatgtaaaa agtaataaat aggtaaaata tgtaagaatt
    55981 atatgttata atatttttag caagaagatg taatctacaa tttatagagt ggagttcatg
    56041 tatcaaaaaa ttatcctccc gacgctaaga agggaggtga atatacatgg ataatttttt
    56101 acaaggcata ctagcaagtc tatctgctag cttaatagtt tatatagcta gcaaactatt
    56161 tagaaagcgt aaaaaaccac tcaaagcggc aactaagagt ggttgggaat ttgatttaaa
    56221 aatcagattc cataaaacta agtaatttct aaattatgaa ctccactcta cagcaaaata
    56281 gattgtagtt cttcttgctt ttattatatc acaaattggt acagatattc aaaaataata
    56341 tttttatgat ataataaagt catagaaatt ttgcagtgtt cgattttttg aagaaattaa
    56401 ggcttaacag ttgaaatata aggcattgag gacttgtgat aagtgttatc aattgcacta
    56461 ctcatggttc actgcaaatt tgagagattt gtatatgtgt aggcattgga aatactcaat
    56521 ttattttggg gttttatatt aactatatgg aatgtaaata atgaactatc taaaccaagg
    56581 tcataattta attgagtttt atattaacta tatggaatgt aaattgtatc caaaaaatta
    56641 caataatcat acgaactgct aatataagca gtcaatatat gtgtacattt atccatttta
    56701 tctctaaaca tataaattat tatcccctta aaaaactttc ataaaaacaa aaagaacaat
    56761 atctctttgg caactggctg acataactca taagatattt atataagtct tagtccctat
    56821 agctttgcgt cactatattt ctctagtttt gccgatttag ttttattcta taactaaata
    56881 atacaataga attagttatt attcaacaag attgtttgaa aattgaataa atttataggt
    56941 atgaacgtaa tttcacactt aaaaacagac taaatacgta gaatatatag aaaaatttac
    57001 ctgtagtttt tgtgataaaa agctcctatt attaatttaa gctagataag tttgaggaag
    57061 tgacataaat tgaaagaagg aatatattat gttgaataaa aaattaccag atgcagaatt
    57121 aaaaattatg aaatacatat ggaatactgg ttataaaact gtaatatcaa aagatgttgc
    57181 agattaaata gaaaaaacat atgcttggaa gcatacaact acaattacac ttttagctag
    57241 attaactaaa aaaggttttt tcatatcgca gagaattggg aaacatgtac actatagagt
    57301 attaataaaa gagaaagaat atctcaaaat agagggaaaa agaatttttg gaggtctaca
    57361 taacaatcca ttatcagaac taatttcaaa attgcatgat ggagaagaaa taactgaaga
    57421 aaaaataata gaaataggaa attggataaa aagctggaaa gatgaagatt aataaataaa
    57481 aggtagctac taatataagt gctacctttt ttcttgtgga taatattggg gataagttgt
    57541 ggaaaatttg tgtaaaaatt gtggataaaa tattttgctg acatttaact gacataagta
    57601 ttagattttg atttaaaaat gttgaaatat caataaataa ttaaatagaa gttattagaa
    57661 ttaacaatag attccttttt caaagaagtc tttgatttca tcaaggttat cagtttttcc
    57721 aagtgctaag ataagtttta ttctagcttt ttgacctggc aagtccccac caaatataca
    57781 accaagattt tttaaatctc taccagaacc aaagtaacca taactatcga atactctacc
    57841 tgaatgacat ctagaaacta taactacagg aagtcctttt tctctagcat attcaactcc
    57901 agaaagcatt tgaggaggaa tattacctct gcccattgcc tcaataacta tacctttata
    57961 accactatca acagcaaatc ttataaagtc agcattttct cctacatatg ctttaaataa
    58021 ggcaacttta gattctacct tatcagtatc tataattgtt ctatttacaa tatctcttgt
    58081 aaggacaagt tcgttgcagt caattattcc taatgggcca ctagttagag atttaaatgt
    58141 atttagagaa agagtatttg ttttagtagc ttcagaagct aataatactt cattgtttaa
    58201 aactacaagg actccttttc ccatagcatc tttggatact gcagtacaaa ctgatgcaga
    58261 taaattactt gaaccatcat atccaagttc agaactactt ctcatagcac ctgtgactat
    58321 tacaggtttg atgttgtcaa ttgtaaggtc tagaaaataa gctgtttcct ctaaactatc
    58381 tgtaccatgt gttattacaa cacctgtaat atcttttctt gctaataaat catttacata
    58441 atttttcaat tccatcattc tataaggtgt tatgtgaggt cctggaattt catcaaaatt
    58501 aaaaacttca acatctgcaa ctttatcaat gttggtaacc attgatagta tttgttcacc
    58561 tgataaagtt ggtatagctg ctccgacttt atcatctact gtcatagata tagttccacc
    58621 agtaaaaact atggcaactt ttttcttaat actcatacta ttctctgcct ttcataaata
    58681 aaatttataa ttccgatagg attatatcaa aaaaagtagt taaggtctac ataaactctt
    58741 ttattcatat aatttagata tttcagtgta atataattat ttcttaattt tttgccattc
    58801 aacgatataa ccatttaata tctctcttat ggcttttcct atcttagtat aagcttcatc
    58861 atatagattt gctagagata ttctaattga ccattcagga ccatagaatc catttccact
    58921 aagtaaaact actgaatagt cattagctaa tttataaaga acatctactg gagatgcagt
    58981 tttacttata aatttaaata agtcttctcc gtagtttaat ttagcccatt cttcaatatc
    59041 aaattgagtg taatatgcag cattgttttt gttttcatta attggaagct ctaaagcttc
    59101 aaataatagt ttttgacgtc tatggcagat actcatattg agttttttgt aggcatctac
    59161 tttgtcaatc aatgcaaaag cagagaaaaa tgccatttgt acttgctgtg gagtagaaag
    59221 accagcagta tggttgagag caacaagtct gctatcagca acaactcttt ccataaatga
    59281 aaggctagaa gggttaagac tcatatcact gtaacgctta tctactgatt tttttaattc
    59341 tccagttaaa tcatttattt tcttatcaaa aacatttttc ttatgaaggg caaatgtacc
    59401 aagtctccat cctgtaactc caaaatattt tgaataagaa tatactccaa cagtgttgta
    59461 aggtagtttt gacattaagg atgaaaaatc ttctacaaaa gttccatata catcatctga
    59521 tattatcatt aaatctttat tataattttc aattacatct attagattat tacaagatgt
    59581 ttcatccata gctatagaag caggattatt aggattaaca acgaataaag ctttaataga
    59641 tttgtcacga agtttctcta attctttttt tgtatattgc catgtatgag cacctttttc
    59701 atcaacttca ttagcattta tatttaccac tttaaagtcg taacgaggaa ggttaggtat
    59761 ttcaagataa ggtgtaaata ttggagtcat taaggctatt gtatctcctt tttttagaag
    59821 ttcatttgcc attaatgaat caaatatata gcacatagca gctgtagcac cttcaacagc
    59881 aaaaatttct acatcaccat atttacctga tatatcatat ttcatttctt gaactaggta
    59941 atcgtgtact attttttcca tatgaggtaa cattctatct ggaagtggat agttatcacc
    60001 aataattcca tcagctagtt catgaacaaa ttcatctcca tcaaatccaa gttcatttat
    60061 accaaagttt ataatatctc tagctaaata tgctccaggc atatctatat tttcatttat
    60121 aaatttaaaa aatctttctt taataccctt tttttgaggc attccagcca aatgacctaa
    60181 atcccaatta gaacgagttt cagtaatagc gaaatgacca aaagtaaaaa aggcttctct
    60241 agcagttgaa caagtccagt ttggatttcc acgacctgca tcaagtaatt ctctgttatt
    60301 ttcttttgca gtgtattttg caatatctat taatttgtcc ttaaattcaa aagggcttat
    60361 ttttccatat atgtttttga tttctttata agtatgattt ttcatgttaa attcctcctt
    60421 atgtaataag tttcacattt ttataataaa ataaacaagt tgcaaaaatg attatacttt
    60481 aattataata ggtttataga aaaagtaaag tatgaaatta aaataataac tctatttagc
    60541 cttattaaat atatgttaaa ataaaaaata ccttttaata aaaaatgatt gttatataaa
    60601 ataaatattc aatttaatta tcaccttagt ttttttattg tattttatta tatatgtata
    60661 gttttctaat tttgaataaa aataaagtaa aactttcttt tatattgaag ttgtttagta
    60721 agacatgtat tttattaatt tattaatctt aataatgtta aataaaatca aaaaaataga
    60781 ttttgggtaa ataaaataat ataaaatata aattttaata aaaataataa agtatatatt
    60841 attgctttaa taagaatgta aaaaatattt ttaaattatc atttaatgta atttaaatga
    60901 attgaagata tatgtttatt gtatgcaatt gttgtatttt taggtttttt gtgattaatt
    60961 aaaaaattat aaaaatcgtt tacatgttga tttttaaaaa aaaaagatat agaattatat
    61021 agaaagtaat aaacaaaaat tggatttacg ggagtggaat gaaatgatga atgttacctt
    61081 aacagatgtg aaagaggcta gagagaccat taagaacata gtaaaaagaa ctgacttact
    61141 tgaaagtgtt aaacttagcg aaaaaacagg agcaaatgtg ttctataaat gtgagaatct
    61201 tcaaaaaaca ggttctttta aattaagagg agcttgtaac aaaatagcga gtttaacaga
    61261 tgaagaaaag gcaaatggag ttatagcttc aagtgcaggg aatcatgctc aaggagttgc
    61321 attgggagca aaaatgactg gtataaaatc tacaatagta atgccagcaa ctgcgccttt
    61381 agcaaaagtt agtgcaacta aaggttatgg agcagaagta gttttaaatg gtgcggttta
    61441 tgatgatgca tatgcaaaag ctgttgaaat acaaaaagaa acaggagcaa ctttcttaca
    61501 tccattcaat gataagtatg ttatagcagg acaaggaact atatctcttg aaattttcga
    61561 acaattagac aataaagttg atactatact atgcccagtt ggtggaggag gaatcatctc
    61621 tggtgttgct gttgcagcaa aagcattaaa tcctaatgta aaaatagtcg gtgttcaaac
    61681 tgcaaatata ccttcaatga aagagtcaat aaaaaatgga aaagtaacaa cagcatttaa
    61741 tgatactaca atagctgatg gtatagctgt taaaactcca ggagatttaa cttttgaaat
    61801 aataaatgaa ttggttgatg aaatagtagt ggttgaagag acagaaattg cagaatcaat
    61861 attatttatg atggaaagtc aaaaaatagt ttcagaaggt gctggtgctg tatgtacagc
    61921 tgctatatta agtggtaagt atgtaccagc aaaagatgaa aatgtagttt gtataatatc
    61981 tggtggtaat atagacataa atacgttata tagaataata ggtgttgctt tagctaaaga
    62041 aggaagaaga tattcattta gtacaattat ggaagataag ccaggaaact ttgctgagtt
    62101 aactagaata ataagtgaga atggtggaaa cattttaagt gctaatcaag gtaaattatc
    62161 agcaggggaa gctctaggaa aacaaagtgc ggaatttata ttagagacaa tagattatga
    62221 ccatatagct agaataaaga aagctataga agaaaaaggt tttaaaatta tagaattata
    62281 ataaaaagtg tatataatat acaagaaaaa tagtataata gtgtatgaag atacatttta
    62341 tgtgtattag attaacgaat taggaggatt ttaaaatgaa acatgaagta atacatacta
    62401 atgatgcacc agcagcttta ggaccatatt ctcaagctat aaaagctgga aacttattat
    62461 ttgtatctgg acaagttcct cttgtaccag agactatgga agtagttgaa ggagatgtac
    62521 aagctcaaac tgctcaatct ttaaaaaatc ttaaagctat attagctgaa agtggagctg
    62581 atttttcaaa tgtagttaag actacagtat ttataaaaga tatgaatgaa tttggggcta
    62641 taaacgaagt ttatgctgaa tattttggag aaaataaacc agcaagagct tgtgtagaag
    62701 ttgctagatt accaaaagac gttaaagttg aaatagaagt aatagctgta ttataaaaat
    62761 acaatatttt taatttgaaa aggctgaaaa actatattct agtgattcag cctttttata
    62821 aaaaaataaa tcataataaa gaacatgata aatattgtcg aattaaaaaa taaaattaaa
    62881 ataaatattg caatattttt gagaaggttt tataatgagt atataatttg aaacgtgtaa
    62941 ctattaaatt aggcatgagt tcttggatat ttttatcctg aggattcatg ccttttttgt
    63001 attgaaattc aaaaatctat ttaataagga ggaagtactg tgttaaattt tttcaagaaa
    63061 aataaaagct ataagttaca tgcagtagtg agtggtaaca gcattaatat tgagaaagta
    63121 aacgattctg tgttttcaaa aaagttaatg ggagatggag ttgcaattat tcctaatagt
    63181 aatgttgttg tagccccttg taatggtaaa gtaactgtgc ttacagaatc aaagcatgcc
    63241 tttggtatgg tctcagatga gggtgttgaa atattagtcc acataggaat tgatacagtt
    63301 agtttacagg gtgaggggtt taaaaatgaa gtgtctcaag gagatactgt taaaaaaggt
    63361 agtccaataa taagttttga aagagaaaaa ataaattctc agggaattga ttgtacaaca
    63421 ataataattg tattaaatca tagcgaattt tcagaaataa attgcatggt agaaaatgaa
    63481 gttgttgcag ggcaagatac tgttattgaa attatgaaat aataggaagg gattaattca
    63541 atatgagagt agtaaaaatc tttaataata atgcattatc tacagtaact aatgatagga
    63601 aagaagctat tttactaggt cttggtatag gttttaataa acgtccagga gataaagtta
    63661 atgaagataa aatagaaaag atttattatg ttcaagacca catgcaaaca aaatttttag
    63721 aattattaaa aaatgttagt ccagaagtga tggatgcttc acaacaaatt ataagtctta
    63781 taccaggaaa tgagggaaaa tttaataata aagggatatt atctttagtg gaacatatta
    63841 gttttgcaat agatagaatg aaaaataatg tttttttacc taatttaatg cttttggata
    63901 taaagatgat gtattcaaaa gagtttgaat taggagtaaa agctttagaa attatatata
    63961 gagtatgtca tatccatttg cctgaagatg aagcaggtta catagcttta cattttgtca
    64021 atttacaatc aaatgataat ttggcatatg atactttaaa atttgtaaaa ggtagtattg
    64081 atttgattaa agaatgttat ggattagaat tagacgaatc gagtttaagt acattacgtt
    64141 ttagaactca tcttaaattt cttgctcaaa gaatttttaa gaatgaaatt tgtcaggatg
    64201 ataaaatgat tgaaatgtat gattatttaa ttaacaatca tcctaaaaat aaagaatatt
    64261 tagaaaagtt aaatgtatat attgaaaagg agtttaggta taaattagat aaacctgaaa
    64321 aaatttatct actactacac ctaacaaaaa tcttataaga atgagatgaa ttagtaatag
    64381 aaatatataa ttttaggggg gataagattg aaagaaaaac tacttggagg actggaaaca
    64441 tttgcaaagg caatggttca gccattaatg tatttatcga gtgcaggaat attaatggtg
    64501 gtaggtgtac tacttacaaa tgatatgtta ttaggagtac ttccattttt taaatgggag
    64561 ccaattcaaa tatttggaag cctaatttat aactgtataa tggctattat taataatctt
    64621 gctgttatat ttgctatagg tattcctgct gcactagcaa aaaaagagaa gcacaaagca
    64681 gcgttaatag gatttatgag ttatataata taccttacag cttctaacac tatgttaaca
    64741 tcactaggtc aactagcaaa agaaagcaag atgcttggac tgatagggac tggacaagct
    64801 actattttag gtattcaagt tgtagataca agtgtatttg gtggaattat tttagggtgt
    64861 atagctggat atgtatttaa tcgtacaagc aacaaaacat ttaaaggagc tttacagata
    64921 tactcgggag caaattttag ttttatatgc atgttctttg tatccatttt aattggaatt
    64981 gtgagtaact ttatatggcc attagctcaa caaggaatag cttctcttgc aaatgtcatc
    65041 aaagatacag gtacatttgg tttattctta tatggttttt tagagagaat attagtacca
    65101 actggacttc atcatttaat ctatacacca tttcagtttt ctgatttagg aggagtgctt
    65161 aaagttggtg cagatacagt ttcaggagcc tatgctatag ttatgacaga aatgaattta
    65221 ccagttgagc gtttctcaga tagtatatac tacatggcaa ctggatttac aaagatgttt
    65281 ggttatatag gtataggagc tgcctttatt tatactgcat ataagcaaaa taagaagaag
    65341 gttaaggcaa cgattattcc actaataatt acggcagtat tagcctctgt aacagaacca
    65401 ttagacttta tgtttgtatt ctgtgcacca cttttattct ttgtacatgc agttatagct
    65461 ggtcttttta tagctttatt aaaaatattt gatgttactg cattatgtgg agggaactta
    65521 ttagtatcaa ttataatgaa tactacagta ggcgtagaaa aaacacactg gccagtaatg
    65581 atgatattag gacttgcaca aattattgta tattttgtag tatttagttt tttaattaag
    65641 aaatttaatt ataaaactcc aggtcgtgaa gatgagagta cagctacagg cgaagaacaa
    65701 tcaaaacaac ttaatttaga tgcaggtata gaaaatataa ttgatgggtt aggcggaaaa
    65761 gaaaatatta atacagtaga aaattgcatc actagattac gtgttaatgt aaaagatgaa
    65821 agtaagatta atgaagatat aattaattta acgccaaata gcgggattgt aaggaaagga
    65881 aaagacatac aaataatttt tggacttcat gtgcatgagg taagacgtgc agtagaagat
    65941 tttttaggag cttattaaag atatatattt atatagcaag ggtaaataac agctttaagt
    66001 gctgtttact atttattaaa gatacatata atttatagag gaggaataat tattatgatt
    66061 attactattg ttggtggagg aagcacattt actccaggta ttgttaagtc gattgcgtta
    66121 agagaaaaag agttagagct tgaggaaatt cgcttatttg acattgataa agaacgccaa
    66181 gataaagtag ctgtagttgt caactggata ctaaaagaag atgttaaaac agatgttaaa
    66241 ctaactgtaa ctaattgtga aaaagaagcg tatacagatg ctacatttat atttgcacaa
    66301 atgcgtgttg gtaaatatga aatgcgtgag caggatgaaa aaattccatt gaaatatggt
    66361 tgtgtaggtc aagaaacttg tggttgtgga gggatggcat acggactgcg tacaatacaa
    66421 ccaatgataa aggttattga tgatgtagag aaatatgcta aaaaagatta ttggatatta
    66481 aattactcta atcctgctgc aatcgtttct gaagtttgtc gtaagttaag accaaatgca
    66541 aggattataa atatctgtga tatgccaata gctattatag atatgatagc ggggtcatta
    66601 aatatcaatg acgttcataa tatacgctat gattattttg gtctaaatca ttttggctgg
    66661 tttacttcaa tagattataa acatagagac ttaatggaag agattaagga atacataaaa
    66721 gaaaataaaa tattattacc aaaatcttat atagaaaact taaataaaag tcgtagtaca
    66781 ggaaatcgcc atgctgtaag tagctggata catgtttggg aatctgttta tacaatgtta
    66841 gaaagcttcc caaaatatct gccaaacact tatttaacat actatctttt aagtaaagaa
    66901 acagtggaac attcaaacat aaatcgtact cgtgcaaatg aggttatgga aagccgtgaa
    66961 aaagacttat ttgaaggtgt gagacattac ttagaaactg gagaaatttc tgaaaaagca
    67021 ttttatgcag gttcacatgg agattggatt agtgatttgg cagtttcaat taaaaatgat
    67081 acacgtagtc gttttttagt tataactgaa aatcgtggag ctataccaaa catgccatat
    67141 gatgcaatgg tagaaattcc agcatatatt ggtaaagatg gtccagaagt tattgctcga
    67201 ggtccaatac ctctattcca acaaggatta atgatgcaac aacttaacag tgaaaaattg
    67261 atagtagaag cttatataga aaatagttat gaaaaagctt tacaagcatt cacattaaat
    67321 aaaactattc caagtatgaa tgttgctaaa gaaattttgg atgaaatgat agaagttaat
    67381 aaggaatact ggccagaatt aaaatagtag gataaaagaa agggttttta tttaataatt
    67441 agatatgttt tcagagtctt ttatcaaaat gtattcacaa acatatggca atatattaaa
    67501 ataaagagtg atatttttag aagaatgggg tgatgcaatg aacattataa ataaaatttt
    67561 ttggcaagaa gataggattg gcatgattac aaataattta gaggcagata cacatagtca
    67621 ctgtatgtta caactgttct tagggataga agacagcatt gaaataacag tcaatgaaaa
    67681 attagttaaa tgtaattgta tcatagtaga taaaaatatt tctcattcat tttcagcaag
    67741 aaaaaaagtt tattactctg ctattattga acctacatct atatatgcag aacagcttac
    67801 ctcaaaaatg aatgattttg gctactggat atgtgacaat aatggattag agaaattacg
    67861 tcaacaagga acttttttga tagataactc tagtaaagaa caatatttaa gatttatgga
    67921 aatgcttaat aactatttga atatacctat aactctaaaa cattatgatg acagaattac
    67981 tgaattgctc aatttattac gtacttgtaa ttgtgataat catactattt caagttttgc
    68041 agataaagtg tctctatcag caagtagatt atcacatctg tttaaagaac aaatagggat
    68101 acctttaaaa agctatatct tatttcatca aatggagtgt gcttttagag aattactttc
    68161 tggaaaaaat gttaccgaag cttctatgac tgctggattc gatactccat cacactttgc
    68221 agggacagtt aagcgaatga tgggaatgcc tgtttcttta tctttgaaag atagcgagtt
    68281 tttgaaagta tattaaatat tctcgttata taatgaagtt gtactaagaa aggggctttg
    68341 ttatgaataa agaatattta gaagaaacaa aaatgttaaa ttataatgaa ccacaactta
    68401 agcttttggt atcatcaaag aattggttgg aattagatga ttttcataaa ataaaatcta
    68461 tttatgagtt tgtacagaat gatattttat ttggatataa tgcttctgat atgttaagcg
    68521 caacacaggt attaaatgat ggtatgggac aatgcaatac aaaagctact cttttaatgg
    68581 cattattacg tgcagtgaat atcccttgta ggcttcatgc ttttgatgta acaaaagatt
    68641 ttcaacgagg agctacatcc aaattgattt cactattagc accaaaatat attcttcata
    68701 cttgggtaga ggtattttat caagataggt ggattgctct ggagggtgtc ataactgata
    68761 aaaaatattt agaagcaatt cagaagaaat tttttaatca tggtggaacg tttaaaaagt
    68821 atgcaatagc tacaaatgat ttgaaaaata catctattgg ttgggatggt aaagatactt
    68881 ttatccaaaa agaagctatc gtgtatgatt atggtatttt tccatcacca gacgtatttt
    68941 tttctactca ttcacaacat atgtcaaaat taaaaaattt catatatgta catcttatca
    69001 gaaaaataat gacaaaaaat gtatgtaagg ctagaaacaa ttatattgat aaaaatgaat
    69061 gagtgtttaa gaggtaacta tataggaaat atatagaata attaaaactg tactggttaa
    69121 aatgaataaa aaggagttat cacagagcaa acttaaattg ttcataggat aactcctttt
    69181 ctgtattaac ttctaataat ttgatgtctt tttataaaac atatagatac aattaaacca
    69241 gtaaatgcaa gagtagctgc tattgaaaat gcaacatcta ttccatgaat gttagacagc
    69301 atgtttgaac tcatatgagc tttcttagta gcacttgtca ttatagtaat aagtatagca
    69361 gaacctatag ctccagatat ctgacggagt gtgttattaa ttgctgtggc atgagatata
    69421 agttctctat ctaatgtttt aattccccat gtagttaatg gcattaaaac cattgaaata
    69481 ccaatcatac gaatacaata catcaatgac acatgaatta aagatgtatc tctgcctaaa
    69541 aaagcgaagg ataaagttcc taaaaacaaa catcctgttc ctaatattga tagagcatga
    69601 ggtccatatt tatctaaatg acgtcctgca attggattga gtacaaccat caatatagca
    69661 ccaggtaaca ttaatgaacc tgaagataat gcagaaaatc ctcttacaga ttgaacgtac
    69721 agaggaatca ttaatgtagc agacatcatt gaggcatata caatcataat taatattgta
    69781 gaaataagaa atggtttatt tttaaaaacc ctcaattcta aaaaaggttc tttagatttt
    69841 aattgacgca atgtaaataa aattaatgac ataattccta ttaatattgg taaatatgtg
    69901 gcaatattag tccacccata attaccttga ttagtaaatc caattaataa accaccaaaa
    69961 cctagagatg atagtactat tgaaggtata tctaatttta gtttttgagt ttccccaaca
    70021 tttcttaata gaataaatga tagtataaca tctaatatag caatagggga aagaaacaga
    70081 aataaactat gccaaccaaa tgaatctact aaccatcctg ataaagttgg acctattgct
    70141 ggagcaaatc ctactgtaat accaactaaa ctcatagctg caccacgttt ttcctcaggg
    70201 cataaataga gtataataac ttgtaaaagt ggcattagag aaccagaacc cattgcctgc
    70261 aacacacgag caatcaacat agttgaaaaa ttattagaaa aaagtgcaat aatacatcct
    70321 atagaaaaga tagacattga tgtaataaat aactttctag tactaaatcg atttattaaa
    70381 taagctgttg tgggtacaat aatacctgca ataagtagat aaattgttgt taaccactga
    70441 ccaacatcag cagtaatttt aaaatctgac attatattag gtaatgctgt tgacaatagt
    70501 gtttgactga gagaacttat aaaagcacca gtcattacaa ttgctattat taaatttcct
    70561 tttttattgt tactttgggt ttccataatt tataacgtct ccttttttaa ttgattttta
    70621 atgctgttca ataaaagtac ttttggtctt aaagtgtctt ctttttctaa atatgcaatc
    70681 attgcatcca taattaaaga atcatattgt tgattttttc cctcgacgta taattttttt
    70741 attgcttcta gtaatttagg attttcaagg agagaagtat cagttttgat tagttcttct
    70801 atatcttgta tcgttttaaa aaatagcgat ttattatgaa gggaaaagta aacttcatct
    70861 tcttgtttat gtaaaaacat tgatttttca attaaaggat ttagattatc catattttct
    70921 acaactaaat ctagaatttg tatagtaaat ttggcaataa agtatacatt tatttctctt
    70981 ttttgtacat gaataatgta tataaactga tgaattagag catccaataa gaaaactaaa
    71041 tcaaatataa ttttttctat tttatcacca tagttatcaa ataagcttaa tttgtattca
    71101 ttcaataagt tagctcgtga ttttgtttgc aatttacttg tagccttacg ttgctcttca
    71161 gaaaaatttt ctcttatata ggtatttatg gtagttctag aaaaaatatt tttccataca
    71221 gcaattatct ttctgtttaa cttttctgat gctggaatgt cattattttc atttatggaa
    71281 tgtataattt ctaaaaactt ttcatcataa taattgatta tttcgcagat aaaactttcc
    71341 ttagtttcaa aatgcttgta aaaagtagct ttagacattt tacactcttt tacaacatcc
    71401 gatattgaag tattttgaaa acccttttgg tcaaaaattt tttgtgcaat agttataatg
    71461 agcctttgtt tttctgtcac tttattatct cctttcacgc aaaaacgttt agtactatag
    71521 agtacctaca agttttaata aatatattat acatcatttg gaaaaaaaat caatatatag
    71581 actgtaatat gaaatttttg aaaaatttgt tgttattttt agtcgtataa aaaagaaact
    71641 taagagtttc ttaagaattg tttaaagaag ttgcaataaa ttagtggtat gattatagta
    71701 tgaaagagaa tgtcttgaaa tttttctgta tcaaattcca attatggagg tgtagtatat
    71761 gaaaaagaaa atttgtgtag caataatttt tgctctattt atagtttttg tagaaatgtt
    71821 tattgatagc tctagcgaag gaagtaaaat tatgacagct gaattaagta aatacaggaa
    71881 aggcgtaaat gtattatcaa taaacaatat tatagtagcg aataaaaaat atagtttgcc
    71941 caaagattat tctcctcagg aaagcagtga agcgagagat gctttttata aaatgaataa
    72001 agatgctcaa aagtcaggat taaatttaaa agcttttagt acatatagaa gttatgaata
    72061 tcaagataga ttatttaagt catatgtaaa agaacatgga gaaaaagaag caaatagatt
    72121 ttcagctaaa cctggagaga gtgagcatca aactggactt gcttttgata ttggaggaga
    72181 tgaccagtct tgttgggcaa ataaaaagtt caacaataca aaataagcta agtggttgta
    72241 tgaaaatgca tataaatatg gttttatact tagatatcca gaaggtaaag agaatataac
    72301 aggatatatg tatgaatctt ggcactatag atatgttgga actgaacata gcaaaaactt
    72361 tgcaatgaat aatttgactt tagaagaata tttacatata aattaagaga tggtttgtta
    72421 aaattaggtt aaaataaaaa ataacctaga ctattagctg tgtaaagtga tataatgtaa
    72481 atacaattta agataactac tcgaagggga gtagcttaca cgaaagtggt aattaatcgt
    72541 caagacagta gaaatactcg gttaattagc aagggattcc ttgatagcaa gaccttcaaa
    72601 ataagcatta ggtatgtttg ttttggggtc tttttttatt caaggaattt ttatttaaaa
    72661 aaatttaaat ttcaagtata cataagagcc cccaaacata aaataaattt attgaagagg
    72721 agggcaacaa atgtcttaca aaagtactta tcaagaagta attaaaaatt tagactcaag
    72781 tttatctggt ttgagtaaag aaagagcaga agagttgtta gaaaaaaatg gagctaatga
    72841 attaaaagaa gcagataagg ttcctactta taagcttttc ctagaaagtt ttaaagaccc
    72901 attagtaata attctattga tagctgcttt agtacaaata tttttaggtg aaacagtgga
    72961 gtctataata atttttgcag ttattattat aaactctgtt ctgggtgttg tacaaacaaa
    73021 aaaagcagaa agttcattag aaagtttgaa aaatttatct gcaccaaacg caaaggtaat
    73081 aagagacaat aaaaaaatga caattccagc aaaaaatcta gtagttggag atattgtatt
    73141 cttagaagct ggcgattaca taccagcaga tggaaggctt atagaagcac aatctctcaa
    73201 ggttgtagaa gggatgttaa ctggagaatc agaaccagtt ttaaagcata cagaaaaaat
    73261 aagtgaagat gtaagtttag gtgaccagaa aaatatggta tttagtggtg ctgtagttgt
    73321 ttatggtaga ggttcttttg tagtgactgg aacaggtatg gatactgaaa tgggtaaaat
    73381 agctgggctt ttagaaagtg ctgaaaataa acttacacca ttgcaacaaa aaatagagga
    73441 ttttagtaaa aaacttggtg taggtattgt aatacttgct cttttaatat ttataattca
    73501 agtagcaaga aattacttta tggctggaaa tggagaaatg acacaaatta ttttagattc
    73561 attcatgttc tctgtagcag tagcagtagc agctatacca gaagctctat catcaatagt
    73621 cactatagta ttggctgttg gaacaaatag tatggcaaaa aaacaagcca taataagaaa
    73681 attaccagca gtagaaactt taggttcaac aagtgtaata tgtactgata aaacaggtac
    73741 tcttacacaa aataaaatga cagtagttga ttattatatg tatggaacta ataatgatga
    73801 gttagataaa gaaaaagtaa actattctta tcatgctaaa ttattgacta tggtttctac
    73861 attgtgtaat gactctcata taactagtga tggtaaagaa gttggtgacc caacagaagt
    73921 ggctttaata aattattcaa gtaaaaatga tttagattat gataaattaa gaagtaagta
    73981 tatcagaata aatgaaatac catttgactc agacagaaag cttatgtcta ctgtaaatag
    74041 tatatatgga gattatataa tgtttacaaa aggtgctcca gatattgtgt ttagtagatg
    74101 taaatatgct ttgaaaaatg gttcaaaagt agatataaca gatgagataa tagaagaata
    74161 taaacataaa aatgaagagt tctcaaatag agcacttaga gtattagctt ttgcaataaa
    74221 agatgtccca gaagaagatt ttataccttc aattgatgac gaacatgaca tgacattggt
    74281 tggaataatg gcaatgatag acccaccaag agaagaagta atggatgcag taaaagaggc
    74341 aaagagtgct ggtataaaaa ctgttatgat aactggtgac cataaaacaa cagcagcagc
    74401 aattgcaaaa gagattggta taatggaaga aggagacttg gctcttactg gaaaagagct
    74461 tgatgctttg agtgaagaac aactatatga aaaactagaa aatataagtg tatatgccag
    74521 agtctcgcca gaaaataaaa ttcgtatagt aaaagcatgg caacataaga ataatataac
    74581 agcaatgact ggtgatggtg ttaatgatgc acctgcatta aaacaagcag atataggaat
    74641 tggtatggga agtggtactg atgtagcaaa agatgcttct gctatggtat tggttgatga
    74701 taactttgca agtatagtaa atgctgttga agttggtaga acagtataca gtaatataat
    74761 aaaatcaatt acttatctat ttgcaggaaa cttaggagct atagtatcca tagtatttgc
    74821 agtgtttgca ggttggtcaa atccatttac agctttacaa cttttgttta taaacttagt
    74881 aaatgactct ttaccagcaa ttgcattagg gtttgaaaag tcagaaaaaa atataatgaa
    74941 taaacctcct agacacccgg atgaaagtat actagcagga ggaacattga aggcagttgc
    75001 tataagaggt tgtataatag gtgtagttac aataattgca caatatatag gattacaagt
    75061 atctccagaa cttggagtag caatggcatt ttcaacattg atattagcta gaataatgca
    75121 aacattacct gcacgttcta ataatcaaac agtaataaaa ttaggttttt tcagcaataa
    75181 atacgttata ggagcagttt tagtctgtct tgggctttac tctttaacac taataccagt
    75241 gctaagagga atattctcta taccaacaaa gtttggattg taccagttgg ggatttgcct
    75301 agggttagca ttaatttcat caatattaat ggaacttaac aaattaataa atagaagtga
    75361 agtaaaataa tcaaaaaatt aaagggcata ttgattttaa caatatgcct tttataaata
    75421 gtctaattta ttctatatta tcgattattt ggaatgcttt tataacttca ggtaattctt
    75481 tttcttcagg cattctaaat cttatgaaat ttttatcaag tccagtaaaa tcacttccag
    75541 atataactct tatttttaat ttagcaaact cttgttgtaa atctatgttc ttgtttttat
    75601 gttcaactgt cataattgat acagtatcac tagtttcagc tatattaagg tttttccatg
    75661 gaattaataa ttgatttttt atatcttttg ttttttcttt taattcttct ataaattgaa
    75721 catcatctaa taaatttgct gcaatactac gagatatttc agacacttcg taaggagtag
    75781 aaatcttttt aatagggcta acaagttgtt ctggaagaac tgcatatcct gctcttagtc
    75841 ctgctaaacc gaatcctttt gaaaatgttt ttaaagcaat tacattatca taattattta
    75901 gaagcttgac agctgaattt tcttttggca tgtattcacc ataagcttca tctaccataa
    75961 ctgctatatc atatttagct gcttccttta ctatattttc aattgatgat aaaggtataa
    76021 tttgtcctgt aggattgttt ggattgtcta tgtaaattac tttatattct ggatttattt
    76081 tttcaataaa ttctttttca ttaaacttga aattatcctc tttctttaaa agaacataat
    76141 cataagttgc accatgcatt tttatatctg tctcatattc tgagaactga ggtgaatatc
    76201 ctaaaacttt atctcccttt tcaataaata gtctatttaa aaggtaaata acatgtatag
    76261 aaccatctgc caaacaaatt ctgtcagtat ctaatgctat aaaatctttc caataatcaa
    76321 ctatattatc ttttaaagca attgaatgag gatatcctct tatcatatca aattttaaag
    76381 cattaaaagc ttctacagct ttgtttgagt aagacacggt atttatacct tcaccacagt
    76441 ctatctcgaa tacatcttct gaatgtccag attctattgc ataacttttt ttcaactgca
    76501 tttcaatagc tttgttaatt tttagtttca tactttatca ctccttattt agtcaataat
    76561 aaatgttata aaagttgcta gatttaaaat attatgaaaa tatataatag taaaaatgaa
    76621 tataaatatt ataatagctc actttattta atatgattat atatcatata ttgtaaaatt
    76681 tctagcatta taagactata aattgaaaat attcagaaaa atactacaag aaacgattta
    76741 ttttgctctt aaaataagtt aaatgaatgt tattaatttt gtaaattgaa atagtttaag
    76801 tataaagtta tgatataatc taataagggt aattctttag attatattaa ggaaatatag
    76861 gtatacaata aaaagttatg gagttggatt atgggatata aattaattat aacagatatg
    76921 gatggaactt tattaggcaa taatcataaa gttacagatg aaaataaaac tgcactacaa
    76981 aaagttataa aaagcggtat taatgtaact ttagcaacag gaagagcctt cgattcagct
    77041 aaatgcaatg tagattttct taaagaggac atgccaataa tagcttgtaa tggttcatta
    77101 ataagagagc aaaatggaaa tataatatat tcaaataaga ttgatacaag aacatgttta
    77161 aacattttag atgtattaga taaatacgat atttactacc aatgtaatag tatagattct
    77221 atgctttcga aaaaaataga aggacgtgaa gatagattaa gtgtattttt aggctctgaa
    77281 acagaagtaa ttgtaaaaga tgatttgaga gaagaaatct ttaaaaaaga tatactaaaa
    77341 tttgtaatca tagaagaaaa aaatccatct attttggatg aaataagaaa agaattgaga
    77401 aaagttcaag gaataaagat aacttcttca tggccaaaca atattgaagt catgaatgaa
    77461 ggtgttgata aaggtaatgc agttaaaatt ttagctgaaa aaatgaatat agacagagaa
    77521 gatataattg catttggaga caattacaat gacatagaaa tgataaagtt tgctggtctt
    77581 ggggtagcta tgggaaatgc agaagaattg attaaacaag aagctgatta tgttacagat
    77641 acaaatcaag acagtggagt tgcaaaggca atttataaat tcctagagtt aaaagaatca
    77701 cttagctctt aaaacagtat ataataggaa aaaataggtt gactattttg tagatagtaa
    77761 ttataataca aatagataaa acataaaaat ttagattcag tgaaaaagag agtagatata
    77821 gagaaaatct cagcgaattg ggggatggtg tgagcctaat gataacactt tatattgaaa
    77881 agaactttgg agaatactac ttgaaattca gtatggtagt aggtaatctc gccttaagag
    77941 aatagagtgg gtaaacattg tttaccaact agagtggtaa cacggaatat ttcgtctctt
    78001 ggtctttttg accaagagat tttttagttt cattgagtta caagacttat aattaaaata
    78061 caaaaaatta aaatatgagg tgaaagttat gaaattatgg ggcggacgct ttagaaaagc
    78121 ggaaaatcaa cttatggaag aatttaataa atcttttggc tatgattgtg tactgtacaa
    78181 aaaagatatc gaaggaagtg tagcacatgt tcatatgcaa gtaaaatgtg gacttttaac
    78241 agaagaagaa ggaaagtcta taacagaagg tttaaaaggg atacttgaag atgtagaaaa
    78301 tggaaagtta gtattggatg gagagtatga agatatacat agttttactg agataaattt
    78361 aattcaaaga attggagatg ttggcaagaa gcttcataca gcacgtagta gaaatgacca
    78421 agttgcagtt gatatgagat tgtatgctaa agaaaaagca aatgatttgg tggatttaat
    78481 atctgaattt aaagctacta taaaagatgt tgctgacaaa aacccagtga tgatgccagg
    78541 ctatactcat cttcaaaggg cacaagtagt gacatttaag catcacttaa tggcatatta
    78601 tagtatgttt gatagagatg agaaaagaat aaaaaatgct atagaaatat tagatgaatc
    78661 acctttaggc tgtggagctt tagcaggaac tacgcatgat atagatagaa gtataacttg
    78721 tgaacaatta ggatttaaaa aagtagtaga taattttatg gatggagtaa gtgatagaga
    78781 ttatttacta gagctaatga gtgatttttc aattataatg atgcacttaa gtagattaag
    78841 tgaagagttg atactttgga gttctcaaga atttggattt gttgagatag atgatttata
    78901 tacaactgga agtagtataa tgcctcagaa gaaaaatcca gatggtgcag agcttataag
    78961 aggtaagact ggtagagtat atggaaactt atttggtctt tttacagtaa tgaaaggaat
    79021 accattagca tataataaag acatgcaaga agataaagaa ggttttttcg atagtgtaca
    79081 tactcttgaa atgtgtatac aaataatgga tagaatgata gccacactaa aggtaaatga
    79141 ggataaaatg aaacaagcag taaaaaatgg ttttttaaat gcaacagaag ttgcggatta
    79201 tttggttaaa aataatgttg cttttagaga tgcacatggt atagtaggaa gtatcgttat
    79261 atattgtgaa gataataaaa aagcaataga agatttaaca ttagaagaat tacaaaagtt
    79321 ttcagatgct tttaaggaag atatttatga ttttattgac tatgagagta tattaaataa
    79381 gggaataaaa aagaatttaa aataatgtat ttatttttaa aagctgtaaa tttgaatgtt
    79441 atcatttaaa tttacagctt tttattgtgt aagattttat tatataaaag taagaaactt
    79501 aaaagtagta gtatttaaaa taaagacaaa taaaaaattg ttattaaaat gataattaat
    79561 atcaataagt attgacaaaa ttatttctaa atactattat atataatata aataataatg
    79621 aaaatcaata tcaaataaat gggaggaaat ctaatgaact tattagtgat tggtggtcat
    79681 gaaagaatgg aaaaagatta tatggttatg gccaagaaaa aaggttataa gacaaaggta
    79741 tatactacca tgtcatctaa attaaataat tctataggaa gaccagatgc agttgtcata
    79801 cttacatcaa ctgtttcaca taagatgtct agaacggttg agtctcaagc taaaaaacaa
    79861 gatatattaa tcgtaaggca taaaaatagt agtaaggtag catttaatga atgtctagat
    79921 atggttgatg aatgtctggg aaattgttca aaatgtaagc ttaaagacaa acaaaaacaa
    79981 aaaaatatat aggtataaaa aatacataaa cataaaatta aaagtactga gataatagtt
    80041 ataattatta tcttagtact tttagattta taaggataag tatagtataa attttttatt
    80101 ataatttcca actacttgtt actttttcat atttttttta cttctagctt tttttctttt
    80161 tttcttcttt atatttgaag ctattataaa taaaactaaa agcactagaa tacctgcaat
    80221 aatcttaaca aaagtcatta aaacattatt ttcagtcaaa aatggaagac tgctatcaag
    80281 attatttttt gcaattaagt ctatactgtt ttctagttta ccattattat atacatttaa
    80341 agtaccaacc ttagctcctt tttttatagg caaatcaatt ttatttaact ctgttttggt
    80401 atcataattg ccttgagatt catctttttc taagaatatc ctataactat tttttggttc
    80461 ataaatcaat tcatgttgtt ttgtaaacaa tactttttta gtatctgtgt atttttcttt
    80521 atcaacaata gtagcacact taaagttatc aaatccataa tcaatcagaa tacgtgagtc
    80581 taaatataaa tcagcattag ttgaattaaa tacagcaacg ataactcttc tgccatcttt
    80641 tacagcactc gataaaagac atttaccagc agcatcagta tatccagttt ttataccatc
    80701 aactatatca tattttatat ttataggttg acctttgtat gtaatcttat catgtgatgt
    80761 caaaaactta ttagtgttta caaagtatct ctcataagga tatgcttttg tagcttcaaa
    80821 tttaagtgac ttagttttta caatttgtct aaaagtatca ttattcatag cttctctagc
    80881 tatgagagcc atatcatgag ctgttgtgta gtgatttggg tctggtaatc catgaggatt
    80941 attaaaatga gtattagtag ctccaatttt ttttgcctca gagttcatga ggtcaataaa
    81001 tttttgtaca ttgccaccac caacatatct agctaataca aaagcagcat cattagcaga
    81061 atgaaccaaa agagcatcta gtaactgctt tacagtaaaa gattccccat tttttagata
    81121 catactagag ccatctattt gaggtaaatc tttaatttct attacttgat ttaaatcttt
    81181 aacctccttt agaactaaac aagctgtcca tactttagtt gttgaagctg gataaagctt
    81241 agaatttcca tccttattat atagtatttt gccagactca tagtccatta aaactgcatg
    81301 tttagcaact atagctggtt cattatctgc aaaagagata ttagagcttc ctaaaaatga
    81361 agtaaatatc aataaacaga ttaataaaag tgataaattt cttttcattg tgagcctcca
    81421 aattttaaat tcatctttta gtatagcata tatttgtcaa taattaaaag aaaggagtga
    81481 ttaatatatg aaaaaaaaag taaaatttat ttcgtttttt gttttattta ttttaataat
    81541 atcatcaata atcgtttata aatcgcttat tttcaaagat gatatttatg ttgtaagtaa
    81601 aaaaaattta cctaatgaaa aaacacatgt tgtagagaat gaaattaaag gtgaagataa
    81661 taatcacaat aaagtaacta aagacataga taaaaatttt caaaataata aaataactat
    81721 atacataagt ggagcagtaa atagacctgg aattgtaact atagagtcag acaagagact
    81781 ttatgatgct gtagaattac taggaggaac gacaaaagaa gcagatttaa atggagtcaa
    81841 tctatcagta agattagaag atgaacaaca ttacataata cccaaaatag gtgaagcaac
    81901 atctgttaca agtaatgatg attccaataa accaaatcag aggaatgagt caagaggtga
    81961 gcctaaaaat aaatccaata aaaatatatc taacgaatca aaagtaaata taaatgtagc
    82021 gactatagaa gagttggatt ctctacctgg ggttggagaa gctacagcca ataaaatttt
    82081 acaacacaga gaagaaaatg gacagtttag ttcaattgag gaaataaaaa atgtaaatgg
    82141 tattggagat aaaaagtatg aaaatataaa agatttaatt tgtgtagact aaaatataat
    82201 acaaaactta catatagata tgttattata aatatgtatg tatatgtgtg tacatgtgga
    82261 aatttagtaa aggaggcact tttatgaaaa aagaacaaaa aaaacttaca gagatgacaa
    82321 cagcaggtgg ttgagccgct aaaatagggc cagaggttct ggcatcagtt ttatctcaat
    82381 tgcctaaaaa tgataatata gaaaatttat tagtaggact agatacagca gatgatgctg
    82441 cagtttataa attaaatgat gatatggcac ttatccaaac tttagacttc tttacaccaa
    82501 tggtagatga cccatacgta tttggtcaaa ttgctgcatc aaattcacta tcagatgtat
    82561 atgctatggg aggcaaacct ttggttgcta tgaatattgt ttgttttcca tcatgccatg
    82621 atatggatgt acttgcagaa atattaaaag gtgggtttga taaagtgaaa gaaagtggag
    82681 cacttttggt tggagggcat actgtagatg acaaagagcc taaatatggt ctttcagttt
    82741 ctgggattgt aagtccaaat aaagttttat caaatgctac tgcaaaacct ggtgataagt
    82801 taattataac aaaaccaatt ggagtgggtg tattaaatac agccatgaaa gaaggtatgg
    82861 tagaacaaca tatagcagat aaagtaatag aaataatgat acatttaaat aaatatgcag
    82921 caatgagttt tgagaaattt gatgtaaatt cggttacaga tataactggt tttggtctac
    82981 ttggtcatac tttagaaatg gccaaagctt ctgaagtaag tattgaaatt gaatcaaaac
    83041 atgtaccaat tattgaaggt gcaatagaaa tggcacaaat gggaattata cctgctggta
    83101 tgtataaaaa tatgcattat gtatctaaag atgtagaagt tgtaggaaat atagaagttg
    83161 cagttcaaga tattttatac gacccacaaa catcaggagg gttgttaatt tcagtaaaag
    83221 aagaacttgc agaggaactt gtaaaagata tgaaattaaa tggagcaata gaagctaaaa
    83281 ttataggttc tgtagttcca aaaggggaaa aatatattac agttttataa aatattatag
    83341 agaaagagtg attgttttga gcaaaagaga attgtttgca atgttgcctt cagttgatga
    83401 agtattaagt gataatagaa ttgtagaaat aataaatgaa tatcctagaa gtttagtttt
    83461 agaatctgtg agagaagtaa tagatttaaa aagacaattt atattaagat taaaagaaga
    83521 tgcatcaaac tctgtaacaa tagagtttgg tgaaataata gaatctgcaa tagagagagt
    83581 taagttaaat tactcattat ctttaaaaaa agttataaat gcaactggta cagttattca
    83641 tacaaattta ggaaggtctt tacttagtga agatataaaa gatgaattat ggtgtgctgc
    83701 ttcaaggtat tcaaacttag aatacgattt ggataatgga gaaagaggtt caagatactc
    83761 tcatttaact agtacaataa aaagacttac tggagcagaa gatgtactag ttgtaaataa
    83821 taatgcagcg gcagtacttc ttgtacttag cacaatggct aaaggaggag aagcaattgt
    83881 atctcgtgga gaattagttg aagtaggtgg ttcatttagg attccaagta taatggctct
    83941 tagtggtgca gaattagttg aagttggttc aactaataaa actcatttga aagattacaa
    84001 ggaggccatt acagaagata caaatgtatt gatgaaggtt catacaagta attatagaat
    84061 aatgggattc acagagagtg tttctataga agaattggtt aatttaggta aaaaatataa
    84121 actacctgta atagaagact taggaagtgg agtttttata gatttatcaa aatacggttt
    84181 atcttatgaa ccaactgtat tggattctat tcgacaaggt gcagatgtag taacttttag
    84241 tggagataag atgcttggag gtccacaagc aggtattata gttgggaaaa aagagtatat
    84301 agaaaaaatg aagaaaaatc aacttactag agcacttaga gttgataagc ttacaatatg
    84361 tgctcttgaa gcaacattga gaatgtattt agatgagaca aaagctatag aaaatatacc
    84421 tactttaaaa atgttgacat ataaaattga agaattagaa gtaaaagcaa ataaactatt
    84481 tgaaaaaata actgctttaa acttaaatgc aaacataaat atagaagatg gattttcaca
    84541 agtgggagga gggtctatgc ctcttgaaac tataagtaca aaggtaatat caataactcc
    84601 tgaacatatg aatgtttcat ctttagaaaa aaaactaaga cttagtgaag cacatataat
    84661 agccagagtg tatgataata aatatgtact agatgtaaga actatatttg atgatgaatt
    84721 tgatgtaata gttgcggaat tgagaaaagc atttaattaa ataaatttga gttaataaat
    84781 gttaaattta gaagtataag tatgaagttt taattaaaaa atagctacac acacttgtga
    84841 ctttagtcat agcaggttta gagggggcaa aaaatgaaaa atgtagtaat tggaacagca
    84901 ggtcatattg accacggaaa aactactctt ataaaagcat tgactggaag agagactgat
    84961 acgttagatg aagaaaaaaa gagaggtatt tcaataaact taggatttac ttattttgat
    85021 ttaccaaaca atagaagagc tggaattgtt gatgtcccag gacacgaaaa atttataaaa
    85081 aacatgttag ctggagcatc aggaattgat atagttttac ttgttgtagc agcagatgaa
    85141 ggggttatgc cacagacagt tgagcattta gatattctaa gctttttaaa tatcaaaaat
    85201 ggaatagtgg ttctaactaa gtctgatact gtagatgatg aatttagaga gctagtaaaa
    85261 gaagatattt gtgaaaaaat aaaaggaact tttcttgaag atgctgaaat aattgaagtt
    85321 gattctatat ctaaaagagg tatagacact cttattgaaa aaatagataa aatgagtgat
    85381 gatatagaag ataaaaatga aaactctcca gcaagactta atatagacag agtattttca
    85441 ataaaagggt ttggtactgt aataacaggc actttaatag aaggaaagat aagtatagaa
    85501 gatgatttgg ttatatatcc acaggtatta aaaacaaaaa taagaagtat acaagttcat
    85561 ggagaaaata aagatacagc ttatgcaggt caaagaacag caatcaatat atcaaatgta
    85621 aaagtggatg atataaaaag aggagatgtt ttagctgcac caaactcatt ggaggaatca
    85681 atgatgttag atgtaaaatt atcattggta aatcatttag ataaaggatt aaagcactgg
    85741 gatagattaa gattgtatca tggaactaga gagatacttt gtagagcagt tccactagat
    85801 aaagaattaa ttgaaagtgg agaaagtggc tatgttcagt tgagacttga agaaagcata
    85861 gtatctaaga aaggtgatac gtttgtagtg agacgttatt ctccaatgga aactataggt
    85921 ggaggtgtaa taattgaccc atctcctaaa aaacataaga aatttgatga aaaagttata
    85981 gaagcattaa aaataaaaga aaaaggcgaa ctaaaagata taatagaaga gtacttaaaa
    86041 aggaatttaa aaaattatcc aaatataaaa gaaataatga gctatagtgg tgctcatgaa
    86101 gaagatgtaa aaagagcgct tgaaacactt atatcagaag ataaagtatt tattatagga
    86161 aatatgtata tgcatataaa tcaatacaat aaattaaaag aaaatacaat aaagctttta
    86221 agtgagtacc ataagaagta taggcttaga aaagggatac taaaagaaga agtaaggtct
    86281 aagatagaga gtaattttaa gactagggag atggatattt tattagaaaa attgtctaca
    86341 gaaaatgcta taaaaataga aaataatata gtatctctat tagattttga ggttatttta
    86401 aatgataagc aaaaagaaat agcaaaaaaa atagaaaaaa gattaaaatc atgtggagtt
    86461 tcttctatac tgacaataga tgaagtttct gaaggaaatc ataactatgc tgaagtattg
    86521 gagtcaatga taggtaataa agtagagaaa ttagatgatt tgtatataat ggacaaagat
    86581 atttatgaaa atgcaaagaa cattttgata aattatataa aagaaaataa agaaataaca
    86641 ttaggagaat acagagattt gattgattct agcagaaaga attgcatgat tatattggaa
    86701 aacttcgata gaaacaaaat aactaaaaga gtagaaaaca aaagaatttt gttttagcag
    86761 aaaatgtctg tttttgcttt aaggtataaa gaaggaatta taatgtcgag tatcgtataa
    86821 ttaattagta taaaatatta attaagagga agtgtaaagt attgaagaat ataatacctg
    86881 atgtaaaacc agatataaaa gaaagagaag acatgagcct tttatttata ataatgggct
    86941 cagtactttt attatttatt ataaaagata tagagaaata tgaaacttta ttacatgcca
    87001 tatatacgat atttttagta gctacaggag taattatatt taatactaca aaatttaaag
    87061 tagattcatt ttctacattt ttaggattaa tttttatagc tactggagta ttggagtgta
    87121 tatatttatt taattccttg gggattaaaa ctaagtctat aatggagatt aacatcacaa
    87181 tatcagcaat aacagattta tttcctatac tgggtgtata cttaagtttt aaatttgtta
    87241 aggataacaa acaaatttat tcaagtgtaa ttttatttat agcaactgct acattaacaa
    87301 taacatcttt attcataata tcaggaattt gtgactatct gggaaatggt aaatcacaat
    87361 atgctttagg agttataata tctatattca taataattat ttctatcatt agtggaattg
    87421 aattaaattc aagttcaaat aaaggtaaat gggaatatga tgaaaaaaag atttttaaca
    87481 gaataatagt aataataatc ttatcaagag ttccaaatct attgcatata gttattgata
    87541 atagaaatgt agagaggata ttatcacaaa taataata
//
LOCUS       DAEMHZ010000003        69960 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000003 DAEMHZ010000000
VERSION     DAEMHZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.003"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      complement(136..231)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            454..1341
                     /locus_tag="KPK72_000177"
                     /pseudo
     CDS             454..1341
                     /locus_tag="KPK72_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1359..3047
                     /locus_tag="KPK72_000178"
     CDS             1359..3047
                     /locus_tag="KPK72_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2487429.1"
                     /translation="MITIKNKRRISDSKIQKCFSVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            3059..4126
                     /locus_tag="KPK72_000179"
     CDS             3059..4126
                     /locus_tag="KPK72_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2487430.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            4318..4848
                     /gene="rnmV"
                     /locus_tag="KPK72_000180"
     CDS             4318..4848
                     /gene="rnmV"
                     /locus_tag="KPK72_000180"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG2487431.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            5019..5888
                     /gene="rsmA"
                     /locus_tag="KPK72_000181"
     CDS             5019..5888
                     /gene="rsmA"
                     /locus_tag="KPK72_000181"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG2487432.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            5954..7219
                     /locus_tag="KPK72_000182"
     CDS             5954..7219
                     /locus_tag="KPK72_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487433.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSSDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            7370..8485
                     /locus_tag="KPK72_000183"
     CDS             7370..8485
                     /locus_tag="KPK72_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2487434.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            8671..9567
                     /locus_tag="KPK72_000184"
     CDS             8671..9567
                     /locus_tag="KPK72_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2487435.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            9637..10434
                     /locus_tag="KPK72_000185"
     CDS             9637..10434
                     /locus_tag="KPK72_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487436.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(10489..11841)
                     /locus_tag="KPK72_000186"
     CDS             complement(10489..11841)
                     /locus_tag="KPK72_000186"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG2487437.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            12060..12938
                     /gene="purR"
                     /locus_tag="KPK72_000187"
     CDS             12060..12938
                     /gene="purR"
                     /locus_tag="KPK72_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG2487438.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            13094..13372
                     /gene="spoVG"
                     /locus_tag="KPK72_000188"
     CDS             13094..13372
                     /gene="spoVG"
                     /locus_tag="KPK72_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG2487439.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            13585..14964
                     /gene="glmU"
                     /locus_tag="KPK72_000189"
     CDS             13585..14964
                     /gene="glmU"
                     /locus_tag="KPK72_000189"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG2487440.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            15059..16009
                     /locus_tag="KPK72_000190"
     CDS             15059..16009
                     /locus_tag="KPK72_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG2487441.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      16852..16937
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            17099..17617
                     /locus_tag="KPK72_000191"
     CDS             17099..17617
                     /locus_tag="KPK72_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG2487442.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKMPATTSASDLSELKTYMDTLPDKADIGGKYQLLLVDK
                     KLVLQIGDKDGGATLTKAQSAKLLSDLGENKIYKKADLKDANELNKDDEINNENLYIV
                     LIDNADMDSATA"
     gene            17768..19444
                     /locus_tag="KPK72_000192"
     CDS             17768..19444
                     /locus_tag="KPK72_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2487443.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            19456..20664
                     /locus_tag="KPK72_000193"
     CDS             19456..20664
                     /locus_tag="KPK72_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2487444.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            20679..22391
                     /locus_tag="KPK72_000194"
     CDS             20679..22391
                     /locus_tag="KPK72_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG2487445.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            22384..23292
                     /gene="pilO"
                     /locus_tag="KPK72_000195"
     CDS             22384..23292
                     /gene="pilO"
                     /locus_tag="KPK72_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG2487446.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEGEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            23319..23885
                     /locus_tag="KPK72_000196"
     CDS             23319..23885
                     /locus_tag="KPK72_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2487447.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            23922..24449
                     /locus_tag="KPK72_000197"
     CDS             23922..24449
                     /locus_tag="KPK72_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2487448.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            24470..26008
                     /locus_tag="KPK72_000198"
     CDS             24470..26008
                     /locus_tag="KPK72_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487449.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILEEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPDQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            26108..27190
                     /locus_tag="KPK72_000199"
     CDS             26108..27190
                     /locus_tag="KPK72_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG2487450.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            27171..27953
                     /locus_tag="KPK72_000200"
     CDS             27171..27953
                     /locus_tag="KPK72_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2487451.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGFLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            28055..28705
                     /locus_tag="KPK72_000201"
     CDS             28055..28705
                     /locus_tag="KPK72_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2487452.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            28719..29279
                     /locus_tag="KPK72_000202"
     CDS             28719..29279
                     /locus_tag="KPK72_000202"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG2487453.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAAYAIDSIIRENIDMSMNKYNG"
     gene            29296..32682
                     /gene="mfd"
                     /locus_tag="KPK72_000203"
     CDS             29296..32682
                     /gene="mfd"
                     /locus_tag="KPK72_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG2487454.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            32713..33708
                     /locus_tag="KPK72_000204"
     CDS             32713..33708
                     /locus_tag="KPK72_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2487455.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            33832..34371
                     /gene="spoVT"
                     /locus_tag="KPK72_000205"
     CDS             33832..34371
                     /gene="spoVT"
                     /locus_tag="KPK72_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG2487456.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            34491..36104
                     /locus_tag="KPK72_000206"
     CDS             34491..36104
                     /locus_tag="KPK72_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2487457.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            36211..37659
                     /gene="mazG"
                     /locus_tag="KPK72_000207"
     CDS             36211..37659
                     /gene="mazG"
                     /locus_tag="KPK72_000207"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG2487458.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            37748..38026
                     /locus_tag="KPK72_000208"
     CDS             37748..38026
                     /locus_tag="KPK72_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG2487459.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            38098..38340
                     /locus_tag="KPK72_000209"
     CDS             38098..38340
                     /locus_tag="KPK72_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG2487460.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            38402..38656
                     /locus_tag="KPK72_000210"
     CDS             38402..38656
                     /locus_tag="KPK72_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG2487461.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            38657..39190
                     /locus_tag="KPK72_000211"
     CDS             38657..39190
                     /locus_tag="KPK72_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487462.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            39196..39480
                     /locus_tag="KPK72_000212"
     CDS             39196..39480
                     /locus_tag="KPK72_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG2487463.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            39593..40513
                     /locus_tag="KPK72_000213"
     CDS             39593..40513
                     /locus_tag="KPK72_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG2487464.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIKGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            40603..42975
                     /gene="spoIIE"
                     /locus_tag="KPK72_000214"
     CDS             40603..42975
                     /gene="spoIIE"
                     /locus_tag="KPK72_000214"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG2487465.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            43326..45218
                     /gene="pepF"
                     /locus_tag="KPK72_000215"
     CDS             43326..45218
                     /gene="pepF"
                     /locus_tag="KPK72_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2487466.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLFVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            45332..46297
                     /gene="galU"
                     /locus_tag="KPK72_000216"
     CDS             45332..46297
                     /gene="galU"
                     /locus_tag="KPK72_000216"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2487467.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            46797..48401
                     /gene="rho"
                     /locus_tag="KPK72_000217"
     CDS             46797..48401
                     /gene="rho"
                     /locus_tag="KPK72_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG2487468.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            48554..48754
                     /gene="rpmE"
                     /locus_tag="KPK72_000218"
     CDS             48554..48754
                     /gene="rpmE"
                     /locus_tag="KPK72_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG2487469.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            48899..49792
                     /locus_tag="KPK72_000219"
     CDS             48899..49792
                     /locus_tag="KPK72_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG2487470.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            49810..50658
                     /gene="prmC"
                     /locus_tag="KPK72_000220"
     CDS             49810..50658
                     /gene="prmC"
                     /locus_tag="KPK72_000220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG2487471.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            50702..51766
                     /gene="prfA"
                     /locus_tag="KPK72_000221"
     CDS             50702..51766
                     /gene="prfA"
                     /locus_tag="KPK72_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG2487472.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            51895..52575
                     /locus_tag="KPK72_000222"
     CDS             51895..52575
                     /locus_tag="KPK72_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG2487473.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            52604..53644
                     /locus_tag="KPK72_000223"
     CDS             52604..53644
                     /locus_tag="KPK72_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG2487474.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIILGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            53661..54110
                     /locus_tag="KPK72_000224"
     CDS             53661..54110
                     /locus_tag="KPK72_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG2487475.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            54127..54579
                     /gene="rpiB"
                     /locus_tag="KPK72_000225"
     CDS             54127..54579
                     /gene="rpiB"
                     /locus_tag="KPK72_000225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2487476.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            54615..55244
                     /gene="upp"
                     /locus_tag="KPK72_000226"
     CDS             54615..55244
                     /gene="upp"
                     /locus_tag="KPK72_000226"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG2487477.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            55545..56360
                     /locus_tag="KPK72_000227"
     CDS             55545..56360
                     /locus_tag="KPK72_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG2487478.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            56586..57023
                     /locus_tag="KPK72_000228"
     CDS             56586..57023
                     /locus_tag="KPK72_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2487479.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            57208..57435
                     /locus_tag="KPK72_000229"
     CDS             57208..57435
                     /locus_tag="KPK72_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG2487480.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            57440..57826
                     /locus_tag="KPK72_000230"
     CDS             57440..57826
                     /locus_tag="KPK72_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG2487481.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            57829..58533
                     /gene="atpB"
                     /locus_tag="KPK72_000231"
     CDS             57829..58533
                     /gene="atpB"
                     /locus_tag="KPK72_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG2487482.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            58602..58862
                     /gene="atpE"
                     /locus_tag="KPK72_000232"
     CDS             58602..58862
                     /gene="atpE"
                     /locus_tag="KPK72_000232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG2487483.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            58985..59482
                     /gene="atpF"
                     /locus_tag="KPK72_000233"
     CDS             58985..59482
                     /gene="atpF"
                     /locus_tag="KPK72_000233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG2487484.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            59479..60024
                     /locus_tag="KPK72_000234"
     CDS             59479..60024
                     /locus_tag="KPK72_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG2487485.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            60041..61543
                     /locus_tag="KPK72_000235"
     CDS             60041..61543
                     /locus_tag="KPK72_000235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG2487486.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            61571..62449
                     /gene="atpG"
                     /locus_tag="KPK72_000236"
     CDS             61571..62449
                     /gene="atpG"
                     /locus_tag="KPK72_000236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG2487487.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            62462..63856
                     /gene="atpD"
                     /locus_tag="KPK72_000237"
     CDS             62462..63856
                     /gene="atpD"
                     /locus_tag="KPK72_000237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG2487488.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            63859..64119
                     /gene="atpC"
                     /locus_tag="KPK72_000238"
     CDS             63859..64119
                     /gene="atpC"
                     /locus_tag="KPK72_000238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG2487489.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            64581..64961
                     /locus_tag="KPK72_000239"
     CDS             64581..64961
                     /locus_tag="KPK72_000239"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG2487490.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            64976..65437
                     /locus_tag="KPK72_000240"
     CDS             64976..65437
                     /locus_tag="KPK72_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2487491.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            65458..66048
                     /locus_tag="KPK72_000241"
     CDS             65458..66048
                     /locus_tag="KPK72_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487492.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            66063..67220
                     /gene="alr"
                     /locus_tag="KPK72_000242"
     CDS             66063..67220
                     /gene="alr"
                     /locus_tag="KPK72_000242"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2487493.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            67253..67540
                     /locus_tag="KPK72_000243"
     CDS             67253..67540
                     /locus_tag="KPK72_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG2487494.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            67509..67892
                     /locus_tag="KPK72_000244"
     CDS             67509..67892
                     /locus_tag="KPK72_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG2487495.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            68063..68890
                     /locus_tag="KPK72_000245"
     CDS             68063..68890
                     /locus_tag="KPK72_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2487496.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            68883..69824
                     /locus_tag="KPK72_000246"
     CDS             68883..69824
                     /locus_tag="KPK72_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2487497.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT        28158 a         7491 c        12981 g        21330 t
ORIGIN      
        1 gaatttacat ctttattata tcgcatttta agaaaaaata aaactgtgaa tgctagaaat
       61 atattttatt ttatctctaa gtatatatat tatcccccta aaaaactttc ataaaaacaa
      121 aaagaacaat atctttttgg caactggctg acataattta taagaatttt ataagtctta
      181 gtccctatag ctttgcgtca ctaaatttct ctagttttgc cgatttagtt ttattctaca
      241 actaaagcaa tgcaatataa ctagttatta ctcagcatat acgtttgaaa attgaataaa
      301 tttataaata agaataatat tttattccta aaaaggaaca gagtatttaa aggtcaaggt
      361 aagttagaat agtattgtgg actaacattt gattgtatat cgcttatttc aattgttata
      421 agtactttat ttatttggac taactaataa tatccaagtg gttgtggtaa aagtacattg
      481 cttagaataa ttgcaggact tgaggataaa catgatggaa aaataataat aaatgataag
      541 gatatgacta actcaccacc tgaaagtaga aactttggta ttgttttcca atcatatgca
      601 ctatttccaa atatgaatgt atataaaaat atagcctttg gattagaaaa taaaaatata
      661 agtaaatctg atatagataa gaaggttaga gatattttag aagttgtaga attaagtgga
      721 tatgataaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcactagct
      781 cgtgcgattg ctttagagcc agactttttg cttcttgatg aacctttatc tgcattagat
      841 gcaaaagtaa gacttaaact tagggaacag attaaaagtt tacatagaaa actaggcatc
      901 acaactataa tggtaacaca tgaccaagag gaagctcttt gtttagctga taaaatggtt
      961 gttatgaata atggagaggt aatacagatt ggaactccta aagaagtcta taaaagtcca
     1021 aaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tgatgtgaat
     1081 atgatagcaa ttagaccaga agatataaag gtagaattta atagagactt taaagaaatt
     1141 gatgtaaaag taggagagat tttggatata gaatttagag gattcaacta tagaattaca
     1201 gttgaatatt gttctaagca aataaagttg gacgtagttt caaaggtagt ggaacaaatg
     1261 aaactatgtg taggttctaa aataaacttc aagataccta aagaaagaac catacaatat
     1321 aaatcagagg gatgtgctta atagctagag gtggataaat gataacaata aaaaataaaa
     1381 gaagaataag tgatagtaag atacaaaaat gtttttcagt ttttttagtg attgcattag
     1441 tagtatttat attatttcca ttaatatcac tattaaaaaa tgcatttatg gatacaagtg
     1501 gaagttttgt aggaatttca aactttagta aatatattga aaatcccact cttatagcat
     1561 catttaaaaa ctcaattttt gtatctacaa tttcatcctt aatatcacta tgtttagcgt
     1621 ttatatattc atatgcaata acaagaacga atataaggtt taaaaacttt tttaaaacta
     1681 tgggcatatt tccgcttttc gcaccagcta tgctatatgg tatttcactt atataccttt
     1741 ttggaaacaa gggaatcttt acaattatag gcatagaagt accactatat ggtccattag
     1801 gaataattat ttgtcaaata ctgtttacat ttccacaggc ctttttaata ttatctgtag
     1861 cactttcgat ggctgattat agactttatg aagcagcaga tagcttaggg gctagtgaat
     1921 ttaagaagtt cttcaaaata acaattcctg gtataaagta ttcattagtt agttcgttct
     1981 ttgtatcttt tatacttgca tttacagatt ttggagcagc aaagatagtt ggaggaaact
     2041 acaatgtatt ggctactgac atatataaac aagtggtagg tcaatttaac ataccaatgg
     2101 gagctactgt ctctatggta atgttaattc ctgtactaat agcatttaca gtagataaga
     2161 tagcaagtaa aaagcaaggg atgactataa ctgcaaagtc tatgccatat aagattaagg
     2221 aaaataagtt tagagataga gtatttttaa tagcatgtag ttttataact ttattaatct
     2281 ttattttgat atttgtttca gttataggct cagttataaa attatggcca tatgatttaa
     2341 gctttacaat ggaacactat acgtttaaag gtatatcagg aaatggaatc aaaacatatt
     2401 taagcagttt aaaaatatca attttaacag caatatttgg aactatattt gtatttttta
     2461 gtgcttatat gatagaaaaa gtggataagt ttcctagact gagacaaata tcttattttt
     2521 tatcaatgat accaatggca ttacctggtt tggctttagg tatatcgtat ataacttttt
     2581 tcaattcagc aagtaatcca ttaaattttt tatatggaag tacaacaata atggttatag
     2641 ccaatatagt tcatttttat tcagtttgct ttataacttc aaattcatct ttaaaaatgt
     2701 tggatagaga gtatgaatta gttgcaaaat ctataaatat tccattttat aaggtgtttt
     2761 tttatgtaac acttccaatg tccataacat caatacttga agtggctgta tattacttcg
     2821 taaattcaat ggttactgtt tcagcattaa tatttttata tatacctcaa actcaaactg
     2881 catcaatatc gatactaaag ttagaagaga tagggtatat agggccatca gctgctatgg
     2941 cagtattagt gtttttaaca aatataatag taaaactttt gtatgaattg acaacaaaga
     3001 aattaaaaac taagacacaa aaatggcaac aaaaagaagt tgcatagagg aggtaaaaat
     3061 gaaatttagg aaattagtct ctttaggaat attatcaaca cttttattga gtgtaataac
     3121 tggatgtaat aatgcttcat caggttcaaa tttaaagtct aataataaga taaaaggaga
     3181 gttaacagtt tatacagcaa ttgaagaaga tagtatagat acatatttaa ctacatttaa
     3241 agagaaatat ccagatatta aattgaatgt acttagatta tcagtaggag atataacagc
     3301 aagattatta gcagaaaaag ataatcctaa agcagatgtg ttatgggggg ttgcagctac
     3361 aagtttgata attttagatg acaaagaaat gctagagcca tacgctccga aaggctttga
     3421 taaggtaaat cctgtattta aagatgataa gaaagttcct tcttgggttg gaatagatgc
     3481 ttggatgact ggtataacag taaatactaa agaattaagt gataagaaca taccagttcc
     3541 tagttcttat gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
     3601 agcttcttct ggaacaggat atttaacagt atcagaactt atacaaatta tgggtgaaga
     3661 aaaagcatgg cagtatatgg ataaattgca taaaaatatt ggtgtataca ctcagtctgg
     3721 ttcagcgcca gctatattag cagcttcagg agaatatccc attggtatat cttttggaaa
     3781 tagaggtata aagttaaaag gtgaaggtta tccagtagaa gtagtatttc caaaagaagg
     3841 ttctggatgg gagatagaat caaatgcact tgtgaaaaaa gataatataa aagaagcatc
     3901 aaaagtattt ttagattggg ctataagtaa agatgcaatg aatgagtatt ctaagaattt
     3961 tgcagttact acaattgaca cgggaaatcc ggtgccagaa ggatttccaa agaatccatt
     4021 agaacagatg atagataatg atttaaaatc agcagcaaga aatagagagg ctatactaaa
     4081 taaatggata gaaaaatatg atggaaagac agaaaaagag agctaagcct tacaataaaa
     4141 tttagtacaa aaagcaccta gaatataagt tttatatcta ggctcttttg atatatttat
     4201 ttaaaatgca tatttctata taaaaataaa tagtttagta ccttgattaa attaaataaa
     4261 aaccttaaaa taaagagatg taaagaaaaa ctaaaaacga ataaaaggtg aatttatatg
     4321 ataaaagaaa taatagtagt agagggtaga gatgacgtta cagcagttaa gagagcaata
     4381 gatgcagaac ttataactac aggtggattt ggatttccaa aaggtgtaat ggagcgtata
     4441 aaagctgcta atgaaagacg tggtgtcata atatttacag accccgattt tgcaggtgaa
     4501 aaaataagaa aaaaaatagc atcagaagtt ccaggatgta aacatgcatt tctaccgaga
     4561 gaggaagcta aaaaggatgg agatataggt attgagaatg ctactcctca aagtataata
     4621 gcagctctga ataaagttag aactgaaaat acagaaaaaa gagatgaatt taagcaggtt
     4681 gacttaatta gaaatggtct tataggtaat gaagaagcat cacttagaag agatgcactt
     4741 ggaaaaatac ttggaattgg atatggaaat gcaaaacaat ttttaaatag attgaataat
     4801 tatggagttg aaagagaaga atttatagat gcattaaaac aaatctaaac ataatattta
     4861 atgtatgaaa aatatgtata taaaataaat tgtggaatta cggttaattt aagataaatt
     4921 atttataata tataccttat tttaaatcaa tttaaataca tataaataag tataattaat
     4981 aaaattaaat atcaaaataa gttaaaaagg agtaaataat ggatagacta tcttcacata
     5041 atgcaacaaa ggaagtagta caaaaatata attttaaatt ttcaaagtct ttaggtcaaa
     5101 atttcttgat agatagtaat ataattgata aaatcttatc tggagctaga atcacaagag
     5161 gagataatat catagaagta ggacctggta taggaactct tactcgtgag atgggtaaaa
     5221 tagcagaaaa agtagtggct atagaaatcg atagaaattt aattcctata ttgaaagata
     5281 ccttgtcaga tttagataat acagaggttg taaatcaaga tatactaaaa gtagacatac
     5341 aggaattagt aaaagataaa ttaaatggtg ggccagttaa gcttgtagca aatcttccat
     5401 attatataac tactccaata gttatgaaat ttttagagga agacatacct gtaacagaca
     5461 tagtagtcat ggttcaaaaa gaagttgcag atagaatgaa tgctattccg ggtactaaag
     5521 attatggggc attatctata gctgttcaat actattgtga tacagagatt gtggcaaaag
     5581 ctccaagaca tatgtttata ccacaaccaa atgtagattc cacagttata ggtctacatg
     5641 taagagataa aagaaaatat gatgttcata atgaagacat attttttaaa acagtaaaag
     5701 catcctttgg acaaagaaga aaaactcttc ttaattcgtt gggaggtctt ggttttctta
     5761 ataaagatga aataagagaa atattaaaag aagctaatat agacgaaaaa agaaggggtg
     5821 aaactcttag tatagaagag ttttcagttc tttcaaatat aataaataca aaagtatctt
     5881 ctaaatagaa ggtacttttt ttaattttgt taacatatat tgtttagaga aactgtgtta
     5941 aggggggatt gcagtgggtt ctaaaagaaa gtttaaaaga gactatataa tactagaagc
     6001 taaagacata aactttagat acaaagaacg agtactgcca aaagcttttg caaaagttga
     6061 agtaaatgat gaaaaagcaa ttattgcatt atatgtagaa aatctaaaat atgttaaaga
     6121 tgggtataaa gcagtagcaa taaaagatga ccatggatta atagacttag gaaatataat
     6181 tttaaatgaa caaggaagag gtgaatttgt tttagattta gaagattatg atgatgagat
     6241 aaaaggtatt gcattattgt atgaaagaaa tataccttta attggattta aaggaaataa
     6301 gcttgaaaat tatgaggaca tattatttca agaggtagag gaagaagaat atgaagaata
     6361 tgaagagttg gaagaagaag acgaagagat agaggataat gaggaaagtg atagagacga
     6421 aggtagtgaa gaagaatata ttatagaaga atatgatgaa gatgagtatg aagactattg
     6481 ggaagatgat gaagatattg aagaaggaga gttaaaagca agaacggaag tagaagattc
     6541 tagtgatgag gaaatagaag agttcaataa ttataaggaa atagaaaatt ataatagggt
     6601 taaagtacag gaagaatata tagacataga atatgatgag gaagaagagg aagaagaaga
     6661 agtttttgaa gtaaatagag aagaagatag gtttgtttcc tcaaaaccta ctaaatcaaa
     6721 gaaagttcaa aaagatcaga ataatattga aaaaataaaa agagctaatg gagtaagtca
     6781 aggagagagt ggaaataaac aaaccagtac attacttatg ccaagacaaa taaaaaaagg
     6841 tttgaaatta tttaaagaag taaaaccttt tgtaacagat tatatagata agacaagatg
     6901 gtggaaaata gagataaatc ctacaacatt gtgtggatat acaatgcctt atttaggata
     6961 tttaaacgtg ttgaattaca ccatgtatag tgatgcagtt ttacattcat ataaatacag
     7021 acactattta tttggagttc agtatgatga atataataag agaaaacatt atatttatgc
     7081 tatcccagga aatagaaatg aacagccaga caatggaagc acaggatttt taagatatca
     7141 agcttgtgat aatagaacaa atagtttagg atactggcta tgttttatag attctagaca
     7201 aagaaatata gttaggtaaa aataataaaa atgaaaaaat ttagactcaa ttcttatttt
     7261 actgaaaaaa ttaatgaaat gtagtacaaa actttaaaaa ctattgataa tatttaaata
     7321 ttacactaca atatatcttg aaatacctat tatattatgg aggaaaatta tgataaacga
     7381 gcaaagaatt ttaaatgagt ttcttgaatt agtacagata gatagtttat ctttaaaaga
     7441 ggggaatgtt gctaaagtat tagttaaaaa attagaagaa ataggttgtt cagtagtaat
     7501 agacaatgct ggtgaaaaag caaatggaga aactggtaat ataatagcta cattaaaagg
     7561 aaataaagaa ggaaagaaaa tattatttag ttcacatatg gatacagtaa ctccaggtat
     7621 aggggtaaaa cctatagtgg atgaagcaaa tggaataata aaaagtgatg gtactacaat
     7681 attaggttct gatgataaag caggtatagc agctatatta gaaggcttaa gatatataaa
     7741 tgaaaataat atagaacata ctgatataca agttgtattc tctatatgtg aggaatgtgg
     7801 attagttgga gctaaaaatt tagactatgg aaaaatagat tctgaatatg catttatatt
     7861 agatagtggt ggttctccag gtgaaataat agtaaaagct cctgcacaag atgtaataaa
     7921 tgttaagata ttaggaaaga ctgctcatgc aggtcttgaa cctgaagctg gaataagtgc
     7981 tataatggta gctgccagag caatagaaaa tatgaattta cttagaatag acgaagaaac
     8041 aactgctaac ataggaatta taaacggtgg aacagctaca aatatagtaa caggagaagt
     8101 taatataatt gcagaagcca gaagtctaaa agaaaataaa ttagatgttc aaactaaaca
     8161 catggtagag acttttgaaa aagctgcaaa agattttgga gctcaaattg agatagatgt
     8221 caatagagca tatacaccaa tagatgttag tgaggattct gaaataataa aacttgcaaa
     8281 gaaggctttt tcaaacttag gtatagaagg tcatactgaa tctacaggag gaggtagtga
     8341 tacaaatata ttaagtaaga atggtattga agctataact ttaggaattg gtatgaaaaa
     8401 tgctcataca ctaagtgaac atatagctat aaaagactta tatgattctg ctaaaatggt
     8461 agtggagata ataaaggaag cataaattta aataatagtg tataatatat atatcgccta
     8521 gcttaagcta ggtgattttt cattttatag aagattgata atctctattg aaagtaaaga
     8581 gtatacattt gcatataaga gagtgtttaa tttatgttca aatgtaggtt cttaataata
     8641 tttagattgt ttcataaggg aggaataaat atggaaacaa ggtatgaaga agcaaataaa
     8701 ataacaatac aatcaatact ttggaatgta gtattaacaa taattaaggt tatagctggt
     8761 gttattggta attctagcgc tatgattgcg gatggattac attcagcatc agatattata
     8821 agttctatag gagtactaat aggcaattat gtatcttcta gacctggaga tagagagcat
     8881 aattatggtc atgagaaagc agagacatta gtatcatttg tgttatcaat attgcttatt
     8941 ttcgtttcta taacaatagg tatagaggca attaaatctt tatttaactt agatgcatta
     9001 agtgttccat caatattacc attagtagta tcagttattt caatcctgat aaaagagtat
     9061 caatatagaa taacaataaa agttgctaaa aagataaatt caccagccct aaaagctgat
     9121 gcttggcatc ataggtcaga tgctttatct tcagtagctg cttttatagg tataggtggt
     9181 tctattttag ggtttaaacc attagaccca attgcgtcag ttgtagtggc tatatttgta
     9241 gctaaagtag gaattagtat acttataagt tctgttaatg aattgatgga tgtttcagtt
     9301 gatgaagaag agataaaaga attaaaattt attgtagcag atacagaagg tgttaaaaat
     9361 cttggtgata taaagactag aaaacatgga gctatggcat atgtagattt gactatatgt
     9421 gtggatgaaa atttgacagt taagcaaggt catgatattg ctaccaagct tgaaaaacat
     9481 ataataaaac atatggaatt tgtaaaaggc ataacagttc acgttgaacc atgtactaat
     9541 tgccaaggaa ataagtgtaa taattaatta aaacttaaaa aaaagtgtaa aaagttatat
     9601 aattgtttta tgattataaa ctctaggggt ggttaaatga gcaagaaaag aaaactaaag
     9661 aaaaaagctg caatattaat aggagtagtt gttttcttta taatatactt tatatccttt
     9721 agaataggaa taaatatgga aaaggataaa agtgaagtgc caaagaaggt agaaaaagaa
     9781 gaagaagata ataggtcttt aaggactcaa ataaaagaga agaagaaggt ttatatatca
     9841 gataaaaata tagaaaacat aaaaattgaa gaagatatgt gggatgaaat aaagttattt
     9901 ttctcagatt ttaagaaagt aagaaatact acttcttctt acgaagcagt ttatgaaggt
     9961 tattctgatg atggagttaa attttctact gaccttaact tttttagagt atatactgta
    10021 aataaagaag aatattataa agtaccagtt agttctaagg atgcttttaa gaaactttta
    10081 gatgagagta tatatacttc gtttgacctt ttaagtcatt ataaagattg gaaagaggtt
    10141 actataactc atggaaaaga aattaaaaaa gttcatagat ggaaatttga tgatttagca
    10201 cataagatga tttcaaagag ggttgtagga aaagttcaac ctgagaaaag taaggaaaga
    10261 agtgactata attttactat aaatataaaa ggtgataatt ttgatgtaaa agttgataca
    10321 atgggaaaag attatgtaaa agttacttct aagaaaaatg tatcttacta tgaggttcat
    10381 acaagtttat ttgattacct taaaaatgag atatttgaaa ttggaaagaa ataagacttt
    10441 aagaaggcta tcaaattaga tatttgatag cctaaattat tttatatatt atttttcaac
    10501 caacaagtct gcaaccttat aaattggacc agcaccagca gttattacta aatcattatc
    10561 cttaacattt tcacgtaagt attttactat atcttcaaat tcttttatat agatggcatc
    10621 tacattgttt tgatataatt tctcaaccaa gtccttagaa tgtatatctc ctgggtcttt
    10681 ttccctagca gcatatatat ctgttatgat aactttatca gcagcataga atgcctctga
    10741 aaattcacct aaaagagatt ttgttctagt ataagtatgt ggctggaaga tacaccataa
    10801 agttgatttt tttaattttt tggcagcagc taaagttgct tttaactcag ttggatgatg
    10861 tgcataatca tctactacta aagcattctt ataataccct ttagtttcaa atcttcttcc
    10921 gacaccttta taaattttta tattttttct tatagtctct aagtcaatac cagaaacaag
    10981 agctgccatt atggcagaag aagcattgta tatgttatga agaccatata cagaaagttc
    11041 aaattctcct aaatctcttc cctcatattc tattctaaat attccatgac cactattgtc
    11101 aaagtgaata tcttttatta cagcatcatt gtcagaatct ctgccgtatt ttataatagt
    11161 agctttaaca tcatacaata tgtcatcaat attttcatca tcgccattta ttatgaaata
    11221 gccttctggg ggtaataatt ttccaaattt gttaaaagaa gccttgattt catctattcc
    11281 agagaaataa tcaagatggt cttcttcaac atttaaaact atagatatct ttgggttaaa
    11341 attaaggaaa ctatctacat attcacatgc ttcagttata aagtggttgg aattacctat
    11401 cttaacatta ccacctatca tacttaagtt accaccaact agtatagttg ggtctaagtc
    11461 tgcatattca aagattgtag ataacataga tgttgtagaa gtttttccat gtgttccaga
    11521 aactgctata gaatttttat attccctcat gatttggcct aaaaaagttg ctctgttcat
    11581 taccaattta tttttttctt tagcagccac aagttcttca ttgtctggat gaactgctgc
    11641 tgtataaaca accatattta catcatctga tatattatct tttttctgac caatataaat
    11701 agttgcccct tggtccctaa gtttgtctaa taggtatgat tcgtttgaat ccgaacctga
    11761 aacttgataa cctttattga tacatatttc agctaatgca ctcatgctga taccgccaat
    11821 tcctataaag tgtatattca taataaaacc acctttcttt ttaattaagt cctacaatat
    11881 gaactatttc tgtggtataa taatataatg ttcaatattt tataattaat atgcatataa
    11941 tattatatgt attttatttt tgaatataat aaaacataag taatattata ccacaataag
    12001 aattgttatg cattagttag tttaacgtta tataaaggta cttgtaggag gaaataataa
    12061 tgaaatttaa aagaacagag agaattggag caatagttaa aatactgtct gataatccaa
    12121 ataaaatata tactttaagt tattttacaa atcaatttaa tgcagcaaaa tcaacaataa
    12181 gtgaagattt attagttgtg aaaaatgtat ttgagaagct acatttaggt aaagttataa
    12241 ctatttcagg agcagcagga ggagtaaaat atatacctaa aacttctata gcagaaaatg
    12301 cagagttctt aatggaatta tgcgaaagaa tatgtgacaa gtctagaata ctatctggtg
    12361 gttttttata cttgattgac ctaatttacg accctacaat agctgctaag ataggaaaga
    12421 tattcgcatc aaatattgag tatgtagatg cagattatgt tgtgacaatg gaaacaaaag
    12481 gtatacctat ggcacttatg actgcaaaag ctatgaattt acctctagtt ataataagaa
    12541 aagatataaa ggtatcagaa ggtcctacac ttagcatgac atatgtgagt ggaaacagtt
    12601 ctaaagttga aagtatgagt ttgccaagaa aagctcttaa accggatagt aaagttataa
    12661 ttatagatga tttcatgagg ggtggaggta ctataaaagg aatggtggac cttatgaatg
    12721 aatttggtgc agaggttata ggtactggag tattcatatc tacaacgaat ccttcagaaa
    12781 aaatggtaaa agattatatt tcgttgattc aattggatgt taatggagat aaaatagtag
    12841 tagaaccaaa cttaaagact tttaaagatg aatatagaaa tgaagattta gacgaagaag
    12901 aagattcaga atttgaattt gagatagatg aagattaata aaaaaaatat ttagaaaaat
    12961 atgtaaaaat aaaaagagga tatccctagt tgttcataga attatttaaa aaacagttta
    13021 tattaattta ttccagcgat aataatttta atataaatta atttctgtca aaatcaaagg
    13081 gggatatttt ggtatgaaga taactgacgt aagagtaaga aaattaacag aagaaggtaa
    13141 gatgaaatgt atagtttcaa taactttcga taatttgttt gtagtgcatg atataaaagt
    13201 tatagaaggc cataatgggc tatttatagc aatgcctagc agaaaagtag gcgaaggaaa
    13261 tttcagagat atagctcatc caataaatgc agaaatgaga caagttttag aagatgcagt
    13321 tttacaagcg tatcatgaag cattagttca atgggaagta gctgctgaat aataataaat
    13381 atataaaatg gagagagccc gaacatttag gctcttttta aatgtagaat ttctattaaa
    13441 atatgtactt tttagtaaaa aagtgctata ttattagtga taatgtatat gttgtataca
    13501 aattgttaga taaatgtatt ataatgtata ttgattaaga gaataatacg aataaaatta
    13561 aattagattg aatggagtgt atcaatgaac tttaaagcta taatccttgc tgctggcaaa
    13621 ggaacaagaa tgaagtctaa atacccaaaa gttatacata aagtatgtgg aaaagaaatg
    13681 gttaaccata ttatagatgt ttcaaaaaaa tcaggggtaa aagatactgt tgtgatttta
    13741 ggacatgaag ctgaggtagt taaagaaaag cttgcagaag aaattataat agccatgcaa
    13801 acagaacaac ttgggacagg acatgctgta aagatggcta aagaatatat taatgatgaa
    13861 gatacaatag ttgtactttg tggagatact ccacttataa aagaagagac actaaagaga
    13921 ctatttgaat atcatataga aaataaatat catgctacag ttcttacaac aagagttgga
    13981 aatccaacag gttatggtag aataataaga gataagaagg gtgaccttct taaaatagtg
    14041 gaacaaaaag atgcaaattc agaagaaaag atgataagtg agataaactc tggaatttat
    14101 tgttttaatg gaaagagtct tagagaagca ctagatttgc taaataataa caattcacaa
    14161 ggtgaatatt atttaactga tactgctaaa ataatgagag ataaaggatt aaaagttggt
    14221 gcatttgcag gttcaactat tgaagaactt atgggcgtta actcaagagt agaattatca
    14281 aaggcagaag aaattatgag acgaagaata aatgaatctc atatggtaaa tggagtaact
    14341 ataatagaca ctaactctac ctatatagaa tctgatgtta tgataggcaa tgatactata
    14401 atatatccag gtgttatgtt acaaggaaaa acaaggatag gttcagattg tataattgga
    14461 atgaattcat ctataacaaa ttcagaaata ggtgatggta cagaaataaa aaattcaact
    14521 ataattgata gtaaggttgg agaaaattct actgttgggc catatgcata cttaagacca
    14581 aagagtgact taggaaataa tgtaaaaata ggcgattttg tagaagtaaa aaatgctatt
    14641 atagaagatg gctcaaaagc atctcacctt tcttatatag gagatgcaca tgtcggtaaa
    14701 aatgtaaata ttggatgtgg agtagtattt gtaaactatg atggtaaaaa taaatttaaa
    14761 tcaatagtaa aagacaatgc gtttataggt tcaaattcta atttagttgc accagtagtt
    14821 gtagaagaaa aaggatatat agcaacaggt tcaacaataa cacatgatgt cccagatggt
    14881 gctttggcaa tagcaagaga aagacaagtt atcaaagaag gatgggtaga aaaaaagaat
    14941 caaaaagacg accaaagtaa gtaattatgt agtttattat tttggctaaa aaatttagct
    15001 cgaaattatt ataaattata ttcattagta ttttaaaatt ttcaggaggg ttacataaat
    15061 gaatactagc ggaagcgaga ttaaaattat tgcaggtaat tcatcgaaag agttagcaca
    15121 aaaaatagct gactatatag gtgtatcagt actagattgt gaagtaggta catttagtga
    15181 tggtgaaata tgtgtaaaca tgaatgagac agtaagaggt tgtgatgtgt ttgtagtgca
    15241 atctactaac agcccagtaa atgataactt aatggagtta ttaatcttaa ttgatgcgtt
    15301 aaagagagca tcagcaggta gaataacagc agttattcct tactatggat atgcaagaca
    15361 agacagaaag gctaaggcaa gagatccaat cactgctaaa ttagttgcaa atttaataac
    15421 tgctgctgga gcagatagag tattaacaat ggatttacat gcagctcaaa tacaaggata
    15481 ttttgatata ccattagacc acttactagg tggaacaata ttagcaaatt attttaatga
    15541 aaagaaaata gaagatttag tagtagtatc tcctgactta ggaagtgtta ctagatcaag
    15601 aaaatttgct aatactttaa atggagaagt tccaatagct ataatagata aaagaagacc
    15661 gaaagctaat gtatgcgaag ttatgaactt aataggtgat gttaaaggta aaaatgttat
    15721 actattagac gatatgatag atacagcagg aacaatagtt aatgctgcaa atgcacttaa
    15781 agagtttgga gcaaaagatg tttatgcttg ttgtactcat ggtgtgttat caggtccagc
    15841 tattgaaaga attgctaatt cagaaataag tgagttaata gttcttgata ctatacaact
    15901 tcctgaagag aaaagaatag ataagataaa gataaaaaca gtagctcctt tatttggaga
    15961 tgctataaga atgatattct ctaatgaatc agtaagtaaa ttattctaat ttattgtatt
    16021 tatgagagtt gtttaaaagg atataaacct tttgagcaac tcttttttga ttttatactt
    16081 atggaatgtt atttttttct tgttagaaag gtatatatta ttgtaaaaag agattatagt
    16141 ttaaaattat gtatatttaa gtaaaataaa cctaataaag gatatattat tcaaaatatt
    16201 atgaatataa aaaatattac aaaaaagtat tgaaaaaatt cagattatgt agtattatgt
    16261 aaatataaat aaagaaagaa agaggtgttt ccaatgggaa ggttagaaga tgaaccccta
    16321 ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga aagtttattt
    16381 ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt tctggattat
    16441 ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg aagagtttcg
    16501 tttatataat gattgtggct gaatcataac tataatagat agaatgttta taatagatga
    16561 ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta gcaaaggatg
    16621 gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga tatataataa
    16681 ttgtagggag atgtttcttt tttttgtata gcataatttt tctagaaatt ctgtttcaat
    16741 atgtaaaaag ttggtatata taaatagtat aaacagtgtt gctaaaatat ggtagatatt
    16801 atataatata agtggatatg tatatacatt atgagttata taatttgaat ttaataaaat
    16861 agaaacgttg atttatgttc tgtaatgtgg gcaccttgga gcatattgag ttagtggtgc
    16921 aaccggctat gaaattgtat ttatttataa atactattat tttcatagct ttttttattt
    16981 aaaaaatggt tatatggtag aaataaattt ggttagtacg tttaaaaaat tttacttaga
    17041 tttttaacca gatatattat aagtaagtat taattaaaaa atttaagggg gaataaaaat
    17101 gaagttaaag aagaataaaa aaggtttcac tttagtggaa ttattggtag taattgcaat
    17161 tataggtata ttagcagtag tggcagttcc agctttattt agtaatataa acaaggctaa
    17221 ggtagcaagt gttgagtctg attatagttc agttaagagt gcagcattgt cttattattc
    17281 agatactaat aagatgccag ctacaacatc tgctagtgat ttaagtgaat taaaaacata
    17341 tatggataca cttcctgata aagcagatat aggaggaaaa tatcaattgc ttttggttga
    17401 taagaaacta gtacttcaaa taggtgataa agatggtgga gctacgttaa caaaagcaca
    17461 atcagcaaaa ttattgagtg atttaggtga aaataaaata tataaaaaag ctgatttaaa
    17521 agatgcaaat gaactaaata aggatgatga aataaataat gagaatctat atatagtact
    17581 tatagacaat gctgatatgg attcagctac tgcgtaagac cttatgttca ttaggtaaga
    17641 tagtataaat ttaatgaaaa atataatata aaagacagga agaaaatctt cctgtttttt
    17701 tatactattt tctttaatgt tcaattatga atgatgatat aattgagata gtaaaggagt
    17761 gaaacctgtg gctaaaaaag ttagaatagg agacaaactg gtcgaaaaag gatatataac
    17821 agaagaacag cttaaatggg cactatcaga acaaaaaaac agtggaaaaa gactaggaga
    17881 atttctagta caagaagggc ttatagatag taacttacta ataagtgtat taaaagaact
    17941 cttggatata gaaagtatat ttttagaagg gacagaaata gacactttag ctacaaaaat
    18001 ggttccagaa aatatatgca aaagatatac tgtatttcca tttaaaattg atggaaataa
    18061 aatatgtcta gccatgtcag acccacaaga cagagaagca gttcaagatg taaggcgtat
    18121 gtcaggtaaa gacgtagaga tatttatatc aagtacagaa gatataaaca aagcaatagg
    18181 tcatgcttat gcacattcag aaataaataa ggcaatgact gagtacaaca aaaatagaac
    18241 tggtggagta agagaaacag taatacttga agaagatgta aatgctgctc caatagtacg
    18301 attagtaaat aacatactag aaaatgctgt tagaatggaa gcaagtgata tacatataga
    18361 acaaagtgaa aattacatgc gtgttaggtt taggatagat ggaatgttaa gagaatatat
    18421 gaggatgaac tcagcaccat ataaggcagt aatcagccgt atcaagataa tgtcagatat
    18481 aaatatatct gaaaagagaa taccacagga tggtagaatc tatttaaaag tagataataa
    18541 acccatagat tttagggttt ctacaatgcc aactaacagg gatgaaaaga tagctatgag
    18601 ggttcttgat aagagtaatt ttatggttag taaagaagta ctaggaatag acgaacatgg
    18661 ctctaagata tatgatgaat tgataaacac tccttatggt cttatattgg ttgttggccc
    18721 tactggaagt ggaaaaacca ctacattgta ttcaatgtta aatcaactaa atacagaaaa
    18781 tagaaatcta ttaacaatag aagaccctat agagtatgaa ttacctggtg taaatcaatc
    18841 tcaaataaat gaaaaggcag ggcttacttt cgccagcgga cttagagctt ttatgcgtca
    18901 agacccagat ataataatgg ttggagagat aagagatact gagacagcag aaatagcaat
    18961 tagagcttct cttacaggac atttagtttt aagtacatta catgcaaata cagcagttgg
    19021 agcaatttca cgtcttttag atatggatgt ggaaagtttt ttaataactt catctgtttt
    19081 aggtgtaata tctcaaagac ttacacgaaa aatatgtgag cattgtaagg tttcatatga
    19141 agcagatatt ggagaaaaaa aggctcttgg tattgatgta aatgaatcag taactattta
    19201 tagaggaaaa ggttgtgaaa ggtgtaataa tactggatat aaaggacgtc taggtatatt
    19261 tgaaatgctt gaaattactc cagaaataaa agagttaata gattcatctg caaatcaaag
    19321 agaaatttta aagatggcta gaaaacaagg tatggttagc ttaaaagagg atatagttaa
    19381 aaaggtttta aatggaaaga caacagtaga agaaatgatt agaataattt tgatgactga
    19441 ttaaaggatg atattatgaa aagttttaaa tacacagtaa taaaagagga cggaaaaaag
    19501 caaactgatg tgattgaagc taacgatttt aatgaagcta gaaatcttct tagaaagaga
    19561 aatcttagag ttattgaaat caaagaaagc aaaaataaaa atataagatt atctagtaga
    19621 aagagaaaaa aagacttagg agctgaccag ataagtcact tttgtagaca gtttgctatt
    19681 atagtatctt ctggtataaa tagtatttct ggattagaaa cattagctag aaggtcaact
    19741 aacattacat taagagaaga aattaatcgt atagtggcag aaataaaagt aggttctact
    19801 attgcagatt caatgctttc atcaaaatca aagtttccaa agttacttgg tgctatggta
    19861 gctacaggtg aagctacagg taaattagat gaagtattaa aaagtatggc ttcattttat
    19921 gcaagtgaac acagggttaa acaaaagctt agaaatgctg caacatatcc attaatagtt
    19981 ttgatttctt catttataat gatttttata tttacaacat ttatggtgcc taagatgatt
    20041 aattcaatta caacagtagg agcaactctt ccacttataa caagaattgt tatggggttt
    20101 ggtatgttta tgaagaagtt ttggataata gtacttttag ttattatatt atttatctat
    20161 cagtttaaaa aatatctaaa aacgtcagtt ggaagggctc ataaagatag agttataaat
    20221 aagatacctg ttttaggaaa aggaataaat tgtatggtag ctacacgatt ttcaagagca
    20281 ttgtatctat ttgtatcaac aggataccca ttagttcaag ggcttgatta tattatagat
    20341 agtgtaaata acactatggc agagaagatt ttagcatcag ctaaagatgg aataacaaga
    20401 ggagaaggct tagcagaaaa tttagagaga tatacgtatt ttgactctgt gttggtacag
    20461 atgatagcta taggggagca gacaggggaa ttagagaata taagtagaca gatggcagaa
    20521 ttttatgagt atgaatccga gatttattta aataggatgg cctcaatgat agagcctgtt
    20581 ttaataattg cagttggtat tatagttgcg atactggttg tatcaatatt tatgccaatg
    20641 cttagtatat atgatgccat gtaggaggga aaggacaaat gaacaaggtt tatgtatcat
    20701 attatggcga ttctatttcc atagttgagg gcagatataa acacgataag gataagtttt
    20761 atgtaaaaaa ttttaatgtg ttttcaatag aggatattgc acaagatttt tctaaggaaa
    20821 agtatgctct attgagatac gcattagaaa atgttaagtt aaaatcaaga catgtagttt
    20881 tttgtttaaa tacaagagat gtaattttaa aaccccaaaa acttcctaat atggacaaaa
    20941 aggatttaga cggatttatg aagctagaga tggacgaaat gatggctttg gagtcagatg
    21001 aatatgtatt ttcgtatgaa gtatctagtg aaacacctga taattcagat ggcgatgaat
    21061 caagcttaaa tcttattatt ggagctatac aaaaggaaga ggtaaatgca attgtagaga
    21121 taatgcatga atttgattta gtattagata gaatagatac attatcaaca gcttacttaa
    21181 gaatgcttaa aaatttagac tacaatgata taatggtaac caatataagt gacaatagca
    21241 caattataaa tatatataag aaagatactt tatttattag tgataatata cctataaaga
    21301 taactgctaa agatatggac atgcaattat taaatgtagt agaagaatca aaaggtctta
    21361 tgaattacta ttcgtcaaga aactttggta aggttacgga tactatatta atattaggaa
    21421 aaagatattc caataaatta atttatgaaa cttttagctc tgcattttca agctatgttt
    21481 ctcaaggcct tactgagata atggatatat ctgatatgat aactggagat atagatgaag
    21541 atgatataaa ttcaattgta gagcttttag gaagtatgat agaacctaaa gggaaaagag
    21601 attttgaaaa tataaacttt ttaccaatgg atatacttag aaaacagata aaagctaaac
    21661 aaatgaaaaa catactgaaa attgctccaa tagttatttt gattcttgct ataccttatt
    21721 tatgtttaat tttttcaaat aatttagcaa ataaggaact tgaaaatgtt aatggtgaaa
    21781 taagaaatat tgaatctgca tattatcaga ttggaaatat agaaaaaatg atacagagta
    21841 aaagcgaaga aataaaggta tatgatatgt taattgggaa aaaggtaaag tgggaaaatt
    21901 tattggatga tatagataaa aatactccaa gtaaagttga actaacaagt ttatcaacaa
    21961 cttatcaaaa tcttggaact aataataaag ctgataattc aaaaagtaat aagactacaa
    22021 cagataaatc aagtaacaat gaaacaaaat caagtacgaa aagtgatagt aatggagata
    22081 aaacagataa tgatttaaat gaagataaga ctacaaatgt gtcagaagca ttaactggaa
    22141 atagtgagac taatagtaat aacacagata aagatgataa aactccattg tatgataaga
    22201 taccaaatgt tataaatata gaaggaaatg ctaaagattc ctcttatgta ggtcaatttt
    22261 tatataaatt aaagggatta tactatttca aggatgttaa attacatagc ataaaaagta
    22321 atgcagaaga ttctaactat acatttagta tgacattata cctgaaagaa ggtgtattaa
    22381 ctaatgaata aagatatatt taaaaaagat ataaaagata tatttaaaat ggaagttagt
    22441 ctaaaaggga ttcttataaa ggttataatt ttattagcta ttttatcata tggttatttt
    22501 ggactttacc ctcaatatca gaaatatatt gctacaaaaa attctgtaaa acaggctact
    22561 gaaaaattaa atatatataa agagaaaact gcacttatgc caaaatatga aaaacaactt
    22621 gaagaagttg aaagtgaatt aaaagaaaaa agcaaaaatc ttttatatga tatgcaagat
    22681 ggtctttttt taataggttt agataaaaaa atgaaggctt ataatattga cttaaccagt
    22741 tatgacgttg gaaatgttaa agaatacgaa catttttatg gtatatcaac aacaataagt
    22801 gttagaggtg attatagaaa tgtcaaaaaa ttaatggctt atttagaaga acaaaaaaat
    22861 gttactcaaa tactagatta tacaatgact tcatatatag aaccagaaaa agaaggggaa
    22921 gaaacaacaa acaataagga tttaattaaa aatgtgaaca taaataaaga tacaaaaata
    22981 tactgggtgg aaggcgaaaa taagtggcat ttatataagg attgtccatt gataaaagag
    23041 ggagagattc taaaaaatga caaactttta gataggtcta ctaattatct atgtaatact
    23101 tgtgtactta gagctgcaac agaaaaagaa gcaaaaaaag aagtcgtagc tccaaaggca
    23161 gaaggtgtag tagatgctac atttaaattt ataatgtaca ctaaagagaa tactacaatg
    23221 atacttgata cagataatcc atcttcatgg agaccaggaa aatataatcc atttaagtct
    23281 agtgcgaaat aaagctaagt aagctttaaa gataggtgat gagtttgtac aaaaataatg
    23341 aaaaaggtct tactttatta gaagtaataa tagctgtttt tatactgaca atagttttaa
    23401 gtatttctta taaagtgttc aatggtataa catcagcagt aaaaaagcaa cagattatta
    23461 cagatgctca agtaaatatt aatctaatta ataagtatct aaatagagat ttggaaaact
    23521 gtaaagaact aactaaaact ggttcaggta ataattatga atacaatata gagatgccag
    23581 ataatgttgt aaaatatgaa gtttctatag aaactaaaaa aaatacagag gtatattctg
    23641 taacgagaat ccaaaacaat acaattgata cagaaaatga agttagagaa gagataatct
    23701 ataatcaacc actagttcaa aacaataaag aaatgaagga aacaccattt aaaatagaaa
    23761 agcaaactgg taaatctatt tatactgtaa gtatatacta taatgaatca gtgcaagaaa
    23821 gtcataaaaa tagcaattta aataacaaaa cttatacatt tgatgttatg tcaagaatag
    23881 gataataaga atttattaaa aaataagtag gttgataaaa tatgagtaaa aagaatagtg
    23941 aaagaggatt ttccttaatt gaagtgttag tagctatggc tattatggga attgtactat
    24001 ttgcattttt taatattatc aatactaata acaaagcaaa cactaaaaat gacacagata
    24061 taacttcctt aaactatgtc caaagtgaaa tagaaaatct aagagaaaag ataaaaagtg
    24121 gagaatttga ttttgatagt ttagataaac tagaagatgg aactgtcgta tatgaaaaat
    24181 taatagataa atcaaaaaaa gttgtatatg ataaagttct tagtgaaggt gatgtgagct
    24241 tatatgatac tccatatgaa aagattacaa caataaaaga tgaagatggt aatttaatcg
    24301 acaaaggaaa tattacaaat aagataaaga ctattgtaga agataaaagt ggtcagatat
    24361 ataaaatagc tgtaactggg aaaagcatga atgactattc aagtaaaaaa gaggttaaaa
    24421 ttgtaactga aatatttaaa gataaataat tttacttggg ggtgagattt tgaggaagtg
    24481 gaataaattt aaaagtgaaa gaggggcagc tttagtttta gttttggttg tagtagcatt
    24541 gctttctatt gttggattga tattttcaaa tcagatagct aatagaataa aatctacaaa
    24601 gactactaat gaaggtatac aagcaaagta tttagcagag acatgtgtgg aaaatagtat
    24661 tgataaagca tatgaaaaat tgtatgatga gcttgagaaa atggataatg agtttaaaag
    24721 tgaaaatcaa gaaaaatcca tatctaggtc aaaattaaga gatatatcag atgaagattt
    24781 taataatcaa gatgaggaaa atattgaagc agaaagactt ggatatatga ataatataaa
    24841 tttctattta aataaagctt cttcagactt ggaaaaagct agtatggaat tgaaaaagct
    24901 atatgattta gatatgttgg actatagaga cattgaatat gtagatgcaa atattatttc
    24961 acatagagat tctatcttag aaatttgcaa gaattatact tctggagata ttagtaaaat
    25021 aaatgaatat attctggaag aagatataga ctcaactaca ttgattgagg caaagttagt
    25081 gaataatgat attcttttaa aaatgttttt agaagaaaat aaaattgaaa atgaacattt
    25141 aaattcagcc ttttcacata cttataaggc attagataac atttctttgg cgatgcaaaa
    25201 tatgattgag tatagacata cgtttcatat agatgagcca aaagtagaag tatctaatgg
    25261 gattccagat tctcaacaat attatgaact aatacaaaat cctataataa atagtatgga
    25321 gtatatctgg aatagtaaat gggatacatt agaaaaccta ttggaaattt taccagatca
    25381 aactcaagga tttaattctt taagagttca tttgagaaat aatgtaagaa aatttgagaa
    25441 gttaagcgat aatatatcta gtggtaaaaa aaatacagca aaaaatttct taaagtataa
    25501 agagttattg tatgaaatat cagaccaatg taatcaacta aaaagtatgt cttatgagaa
    25561 aatacctgtt aaatatgata acatggcttt gataacaact tttgactata tacaaaatga
    25621 gcttttagca gagataaaat gcagattaaa agaattaaaa ccacaagaaa ttgataaaac
    25681 agaaggtatt actataaaaa taccttttta taaggctgac tacgacatga caaaagaagg
    25741 atggcctaaa ttaaaagaaa atggttcagg ggcggagtta agcttaatgg taactggtga
    25801 taaagatgga ataaaagaag tagaagttac agatggtaaa aaaaatatta ttggtttagg
    25861 tgttgaagaa aattcaaatt caaagtataa ggttgacgcc attgtaaact ttaatctaaa
    25921 tatagataca aatgtagttg gaaattatga tataaaagat aaaattttga ttaatcatga
    25981 tataagttca tacaaaaaag taaactaaat ataagaagca acttagtata aataaataat
    26041 aaaataattt tattatttac tactaaaaaa ataagtaaac taatagaaga tatggagagt
    26101 aaatattatg tatatttatg atttattaga gcaaggtata agactaaacg catcagatat
    26161 acacataacc gttggaacaa atcctgtagc tagagtaaaa ggtggatttg taaaactaag
    26221 tgaacaaata ttgacatcag aagtaacaat gcaaatggca aaagacatag cgggggaatc
    26281 tatgtttaaa gtaatagaag aacatggaga agctgatttt tcagcttctc taaaaacagg
    26341 agaaagattt agggtaaatg catataggca aaaaggaaat tacgcaatag caataaggac
    26401 tataacagca gaaattccaa catttgaaaa attaggcctt ccagagagta taaaatcttt
    26461 tactgaaaag cataaaggat tagttttagt tacaggtcca acaggaagtg gtaaaagtac
    26521 aacacttgca agtatgataa atataataaa tgaaaaacaa caaaaacata taattacact
    26581 tgaagacccg atagaatatg tacatcatca taaacaaagt ttagtaaatc aaagagaagt
    26641 aggaactgat acagaaagtt ttcattcagc ccttagagct attttgcgtc aggacccaga
    26701 tgtaatacta ataggagaga tgagagaccc tgaaactgtt tcaatagcat taacagcagc
    26761 agaaacaggt catttagtat tttcaacact acatacagtg ggggcagcta agacaattga
    26821 tagaatagta gacatgtttc aaccatccca acagcaacaa ataaaaacac agttatctac
    26881 agtttgtgaa ggtgtggtat cacagcagtt acttcctaca gcagatggga aaggtagaat
    26941 agcagcaatt gaattgatgt ttgctactcc agcaataaaa aacctgataa gggaaggcaa
    27001 aacatatcaa attccaaaca tgatacaaac tggagttaaa gtaggtatga aaactatgga
    27061 ccaagattta atggaacttt ataaaaatgg aaagattaca aaagatatgg cacttagtag
    27121 gtgtactgac caagagttta tgacaagaat gataggtgga gtaaactaca atggatatta
    27181 taatcgttaa tctatatgta tttatagtgg gtatagtgtt tggaagtttc tttaacgtct
    27241 gtatttttag gattccagaa ggtatatcta tttctagacc accttctcat tgtatgagtt
    27301 gtaatactag actaaagcca atagacttaa taccagtgct aagctattta atgagtggca
    27361 gaaagtgtag atattgttat gagaaaattt ctagtagata tgctatagtg gaattaatta
    27421 caggattttt atttttaaca gtatttatgt tatatggaat atctacatca actatttatt
    27481 atttggttct tgtgtcttta ctgattataa ttacatttat agatattgac cactttataa
    27541 taccagataa aatattggtt tttggagcta tattttctat gatttttaac cttttattta
    27601 aagaaattcc agttaaggat tcaattctag gtgtttttat ttgtggagga agtgtctgga
    27661 ttatagttct tttgattgaa tttgtaataa aaaaagagtg tatgggtgga ggagatatca
    27721 aactatttgc aatgctaggt ttttacatgg gtgttaaaaa tggtcttttg acagccttac
    27781 taagcgttta tgtgggagca gtctatggaa tctgtgtaat aattataagt agaataaaag
    27841 gaaaggaata taattctgtt ataccatatg gaccttttat atctattgga gcattaataa
    27901 caatcttatg tggaaagcaa ttacttgaac tttatttttc tatggttata tagatgatag
    27961 tcaagttaaa taaagtttta atttaaaaaa atgaattaaa gtatttaata aggacaatat
    28021 tattattaac cctattacta aagtgctaca aactatggag tatataataa taatttcctt
    28081 attttttgca catttgataa ataaatctct atgtgttttt tgtgatagag aaaagaaaaa
    28141 tttatacaat acgtttttta tttacataat aactgtagtt ttatctgtat taatatatct
    28201 aaaatttggg attagtgtag aatgtgtaca atatatggtt ttaattccat ttttaataat
    28261 tatatccatt gtagattact acacaatata tgtatatgat ataacaatag ttagtggtat
    28321 aatagttcaa ggtataattt ttttggtctt tttcaatata gttcaatttg attatataaa
    28381 tcatatattt ggactattga taggctttat tataccttat attattgcta aactaacaaa
    28441 aggattaggc tatggagatg taggtctcta tagcttatgt tgctttgcat tgggaaataa
    28501 ttatgggttt tatgtgatag ttttatcatt tattttagcg tttttgtttt ttattatttt
    28561 atacataact aaaatattta aagatgtaga tataagaaag caaattcctt ttacaccttt
    28621 tatttccttg tcaacaattt taataatatt tacagagtat aatatattta atttttatta
    28681 taatttgtta tacaaattta tataacagga ggtaagaaat gtacgtagta gtagggttag
    28741 gtaatccagg aaagaagtat gagaagacta gacataacgt tggatttgat gttatagata
    28801 ttcttgctaa agaatacaat attagtgtta ctaaaataaa gcataaagct ttgattggtg
    28861 aagggagagt aggtactgaa aaagtattat tagttaaacc acaaacttat atgaatttaa
    28921 gtggagaaac attaatagat atatataaat attataaggt tgatttaagt aatatagtag
    28981 tagtatatga tgatatagat ttagaagttg gaaagatacg tataagaaaa aaaggaagtg
    29041 ggggaactca taatggcatg aaatctataa ctaagtgcct aggttcaaat gactttccaa
    29101 gagtgagagt aggtgtatct aaaccagaag ctggtcaaga cttagcagac tttgtacttt
    29161 ctagatttag aaaagaagaa tctgataaca taaatgaagc attagaaaaa gcggcatatg
    29221 ctattgactc aataataaga gaaaatatag atatgtctat gaacaaatac aatggatagt
    29281 gagagggaga tttaaatgaa tgatgttttt ttatatcctt tgcaaaattc aaaagagtat
    29341 aaagatataa taaactgtat aaaaaataca aaaggctcat tattggttaa tggactttta
    29401 ccagtacaaa aacctcatat atcgtattct atatttaatg atttatcacg acaaatgatt
    29461 tttataacga gtagtgattt agaagcaaag aaagtatacg aagatttaag tttttatatg
    29521 gaagataaag ttgaatattt aggttttcaa gatatttatt tttatcactt ggatgcaaag
    29581 gacagaaacg aagaagcgaa gaaattaaaa gtattgttaa aactagcaaa taaagaaaaa
    29641 atcattttag taacttctat tgaggcagtt ttaagaaaat acataccaaa acaagttttg
    29701 ctcgatagtg tatctcatta taaggtggga gatagcttag acttagaaaa gttaactgag
    29761 aaattagttt cattgggata tgaaagagtt tcaaaaatag aggggtttgg tcaatttagt
    29821 ataagaggtg gaattattga tgtattttca cttgaatata caaatccaat tcgtatggaa
    29881 ttatttgatg atgagataga ttccatacga acttttgacg tatattcaca aaaatcaatt
    29941 gataaattgc aacaattctc tataactcca tctagggaat ttatttatcc cgaaaaaact
    30001 acagatgcat tagttaaatt aaaaaaagaa acaactaaaa atactgatga aggtacattt
    30061 caaaatatag attatatatc aagtaagact tattttgaag gtgcagagaa ctatatagat
    30121 tatatttatc cagaagaaaa caaaagtata tttacttatt tagcagatga tgcaatagta
    30181 tttattaatg atataacgag acttaaagaa agatgcgaaa attatataaa tgagtttaga
    30241 gaaaattata aattaaatct tgaaagaggt ctggctatta aaaaccaagg taaattgtta
    30301 tatcactata cagatttaga gtacttagta aaagacaagt ctgtggtatt aaactcgcta
    30361 ttgccaaagt ctataaataa cttttctata aaatctataa taaattttga aagtagagaa
    30421 gttcctacat ttaatggaaa ggtagatttg cttgtagaag aattaaatcg gctgaaatat
    30481 aatggataca aaataatttt agcaacaaat accttagaaa gagcaaataa attaggaaaa
    30541 gatttattgg acaaaggctt agaaacgact atttctaaag atagagatat tgaaattaaa
    30601 tcttcacaag ttataattgt acctgcacac ataaacagtg gttttcagta taaatctata
    30661 aagtttgttg ttataacaga taacgaaatg ataggtgttt ataaaagagc gtctaagact
    30721 agcaataaaa aagttaaaaa aggaaagaaa atagaatcct ttttagattt aagtgttgga
    30781 gactatgtag ttcatgaaaa tagtggtgta ggtagataca cagggattga acaaattaca
    30841 gtcaatgcta ttaaaaaaga ttatatgaaa atagtatatc aaggtggaga taatctatat
    30901 gtgccaatag accagatgga caaagtacaa aaatatattg gtgcagaagt agaaaaagtt
    30961 aagttaaata agcttggaac aaatgagtgg acgaaagcaa aagcaaaagt taaaaaagaa
    31021 attgaagata tgacaaaaga cttgattgag ctttatgcaa aacgtgaaaa aatacaaggg
    31081 tataagtttt caaaagatac accttggcag gcagagtttg aatcgttgtt tccatatcaa
    31141 gaaacagaag accaattaaa ggctatagaa gaaactaaaa aagatatgga atcaagtaag
    31201 gtaatggata ggctagtatg tggagatgtt ggttatggaa aaacagaggt agctattaga
    31261 agtattttta aagcttgtat ggaccaaaaa caagttgctg tattggttcc aacaacaata
    31321 ttggcccaac aacattataa tacattcaaa gagaggtttg aaaattatcc tttgagagta
    31381 gaagttttaa gtaggtttaa aacaccaaaa caacaaaaac agattattga agatgctaaa
    31441 aaaggattag tagatatttt aattggaaca catagaataa tatccaaaga tataaattta
    31501 ccaaacttag gtcttgtagt cattgatgaa gaacaaagat ttggagtaaa acataaagaa
    31561 gctttaaaga aaattaaatc tacagtagat gtacttacac tttcagcaac tcctatacca
    31621 agaacattac atatgtcttt aagtggaata agagacatga gtgttataga agaacctcca
    31681 caagaaagac atcctgtaat aacatatgtt acagagagta aggaaagcgt aattcaagat
    31741 gagatagaga gagaattaag tagaggtgga caggtatttt ttgtatacaa tagagttgaa
    31801 cacattgaag aaatggcaag tatgattcaa aaactcgtac cagatgctag agtggctgtt
    31861 gcacatggaa gaatgacttc taaatcatta gaaaatataa tcctaggatt tttaaataaa
    31921 gattatgatg tactagtctg tactacaata attgaaactg gtatggacat atcaaatgca
    31981 aatacaatga ttatatatga tgcagataaa atgggattgg ctcaacttta ccaattgaga
    32041 ggtagggttg gaagaagttc aagacaagga tatgcatatc ttttgtatga aaaagataaa
    32101 actttaagtg agatagcgga gaagagatta aaagctatca gagaatttac agagtttggt
    32161 tctggtttta aaatagcaat gagagacctt gaaataagag gagcaggaaa tattttaggt
    32221 tctcaacaac atggtcatat ggcagttata ggttatgatt tgtatgttaa aatgcttaat
    32281 gatgcaatta aaaaagtcaa aggtgaacct attgtggagg aaattgatgt tgaaatagat
    32341 ttaagtgtaa atgcatacat tccagataat tatatcaaag atgagttgat taaaattgaa
    32401 atgtataaga aaatagcttc tattgaaaac aaagaagaca tgttggatat acaagaagaa
    32461 cttgaggata gattctctga tatacctaaa ccagtccaga cacttcttac aatagcttat
    32521 ataaaatcgt tatgtaagat attaaaaata gaaaagataa gacaattaaa agatgaaata
    32581 ttattagttc caataacaaa gtatagaact aaacagaaaa taggttataa tatagttaca
    32641 gaattagaag aattattaga aaagatgtgc aaggtaaaat aacttgtaaa tttaagaagg
    32701 gatgttttaa atttgaaaaa agtaataaca ttggtaatag ctatgatttt ggtagtatct
    32761 gtgactgctt gcagttctag taaaggagaa actgtggcaa cagttgaagg tacaaagata
    32821 tcatcagatg aatttaaaaa aactatagct ctttataaag attctatgga acaaacttat
    32881 ggaaaagata tatgggataa ggaagttgaa aagggtgtaa aatacaagga caagtttaaa
    32941 gatttaattc ttgaccaact tataactaca gaagtaatat attctcaagc taaaaaagat
    33001 aacttacttc ctaaaaaaga ggatgttgaa aaaagcttta aagaattaaa agacgctatg
    33061 ggtaaagatg aaaaatataa agaacaactt aagaaattag gtatagatga tgagttttta
    33121 agagaccaac aagagaaaga tttagcaatg caaaactatc aaagtaactt tgctaaaaag
    33181 actaaaatat cagatgaaga aatgaaaaaa tactatgata cacataaaga tgaatttaaa
    33241 aaagatgagg tagaagcttc tcatatttta ttgaaaacag tagatgataa taacaagcca
    33301 ctttcagata aggaaaaagc tgaagctaag aaaaaggctg aagaggctct taaagaagtt
    33361 aaatcaggtg aggactttgc aaaagtagca aaaaaatatt cacaagatac ttcagcaagt
    33421 gatggtggta aattaggatt cttttcaagg ggtcaaatgg ttgctgaatt tgaagatgct
    33481 gctttttcta tgaaaaaggg tgaagtatct gatttagttg aaactcaata tggataccac
    33541 ataattaaag tgacagacag aataaatgag caaacatcat ttgaagatgc taaagaaact
    33601 ataaaagacc aactgttgaa aaataaatat caagaacaaa tagaaaaatt aactaaagaa
    33661 gctaaggtag aaaaagatga aaaagtaata aataaaatca caatctaaga cttaaaaaaa
    33721 gagtaatatg cttaaaaaag ctatatctgg ataagatata gctttttttg tatatttttc
    33781 ttaacaatgg taaaaataaa cttaaagtaa tttatggagg gattaaaaaa tatgaaagca
    33841 acaggtatag ttagaagaat agatgatctt ggaagggtag ttatcccaaa agagataaga
    33901 aagacattaa gaattagaga aggagaccct ttagagatat tcacagcaaa agatggagaa
    33961 gtcatactta aaaaatactc tccaataggt gaattaaatg aattttcgca agaatatgct
    34021 gaaacattag ctgaagtact aggatatgga gtgttgataa cagacttaga ttctataata
    34081 gctgtgtcta agcttcctaa aaaagattat aaagaaaaaa gtataagtga tgagttagaa
    34141 gcaataataa gtgaaagaac agtaaaatat tcaaaagata aaaacatttt accattattc
    34201 aaggaagacc aaaaagaata tactagtcaa gttatcatgc ctataattag tgcatctggt
    34261 gactgtatag gttcaattgt agttgtttca aaggataaag atacaattgc agaaagtgaa
    34321 gaaaaatctt taaaaattgc tgctaacttt ttaggaaaac aagttcaata attttatcat
    34381 aaagaccttt tacatgaagg tcttttgaag tttttgtgta aaatattgaa tatagcatta
    34441 aaaatatggt atattaaagt ttgttatata aacagtatgg aggttatgtt atgaacaata
    34501 acaaaaataa agactccttt ctaaaaggag cccttatact tgggctagca ggtgtaatcg
    34561 taaaaataat gggtggattt tttaggattc cccttggaaa catgattggt tcagaaggaa
    34621 tgggatacta tcaggctgct tatcctgtgt atactctatt tttaacatta gctactgctg
    34681 gatttccaac agctgtggca aaattggttt cggaaaaagt tgcaattgga aattttaaag
    34741 gtgcaaataa gatatttaag gtatcacata cagttttatt cattaccggt ataatatcat
    34801 tttgtatatt attttttgga gccgactaca tagtaacaaa cgtaatgaaa aatccaggtg
    34861 cgttatactc tatgaaagcc attgctccag cattactatt tgttcctgca atgtctgcat
    34921 atagaggata ttttcaaggg agacaagata tgactaagat tgctgtgtct caagttgcag
    34981 agcaattttt tagagttgta ttaggtctta ctttggccta cttgcttatg aagagtctag
    35041 gtcagacttt tggtgcagca ggtgctatat caggggctac aataggttca atagcatcta
    35101 tgctttattt ggtgtttgct tatatgctag ggagaaaaga gcgtagagca gagatagatg
    35161 caagccaaag atttaaagat gaaagagtgt cttatatttt taaaaagctt ttaactgttg
    35221 ccattcctat aacaatagga gcatcagtaa tgccacttgt aaatatgata gataatgtta
    35281 ttgtaataag aagacttatg gaagcaggat ttacatacaa agtggcaaat tcaatgtttg
    35341 gtcaattaac aggtatggca atggccacaa taaatttacc agcagtcata acaactgcaa
    35401 tgagtatgag cttagttcca gcaatttcta aagcatatgc attaggaaat aagtcaaaag
    35461 ctagaaaaga tactaagagt gcagttaagg taactttact tatagtattg ccttgtgcgt
    35521 ttggtatggc ttctttagca ataccaataa tgggtctttt atttccacat gaaccatctt
    35581 ctgtaggaac tattttgttt actcttactc catgtgtatt atttttagga ttaatacaga
    35641 cattgactgg tataattcag ggaatgggaa aaccaatagt acctgtaatt gctttatgtg
    35701 ttggtatgct atgtaaaatt gtaataagtt atactttaac aggtatacca gatattaatg
    35761 tactgggttc agcatttggt acagttacag catactttgt ggcagccatg attaacttac
    35821 tatatgttaa aaaacacatg aatgtaaact tttctaaaaa agaatttata ataaagccat
    35881 ttataacagt tatgacaatg tttataatgg ttaagttgag ttatggtgct ttagttggat
    35941 tcttaggaaa cagcatatct acaataattg caatatgtgt tggtggaatt gtatatgtag
    36001 tagtaatact tggaataggt ggaattaaaa aagaagagat tctaactatg ccaaaaggtg
    36061 ataaattata taaattgtta aaaaaagtta aacttataag ataatatagg aatatatatg
    36121 caaataaagg aaatactata caaatagtaa atctccttat ttgtataata atataaaaat
    36181 aaagtgaaat ttgctaatag gagtgatgtt atgggtaaga taagtattgt aggtcttgga
    36241 ccaggagact attctcttat aagccaaggt gtgttagaat ctttaataaa tagccctaaa
    36301 gtttttttta gaacagagaa acatccaata attgataaac ttaaagaaac tatacaatac
    36361 acttctttag actactttta tgaaaaagag gaaaattttg ataatgttta tttgaaaata
    36421 tctgaattta tcatagaaga atcagcaaag ggtgacctag tgtatgctgt tcctggtcat
    36481 ccaagagttg cagagaagac tgtaagtata atagagagat tagccaatga aaagcacata
    36541 gcagtagaaa tattgccttc tatgagcttt gtagacgcta tgtttaacta tttggctata
    36601 gacccatctg agggatttaa actggtagat gcttttgaaa tagaaaattc atatatagac
    36661 ttggatacaa gcatgataat aactcaggta tatgataaat ttatagcatc taatgtaaag
    36721 ttaaaactta tggaacatta tgactataat caagaagtat gtatagtaaa tggagcaggt
    36781 gtcaaagatt tagaaagtaa aaagtttata aagctttgtg aattagacag aaatgaaaac
    36841 ttttttgact atttaactag cttatatata cccaaaagtt ctaaaaagat gtataataca
    36901 gtacatgact tagaaataat agttaataca cttagaagtc catctggatg tgaatgggat
    36961 aaaaaacaaa cacatcaatc attaaaaaat tctgttatag aggaagcata tgaactttgt
    37021 aatgcaattg ataataatga tatagatgaa atggtagaag agttaggtga tgttctttta
    37081 caggttatat tccattgtca gataggaaat gaggagggct actttgattt aaaagaagta
    37141 gtaaatggca tatgtaaaaa attaattcat agacatcctc atatatttag tggtgcaaaa
    37201 ttagatatgt ctaagtttga gaaaacttgg gaagaattaa agaaagaaga gaaaggcgaa
    37261 agtactataa cagatggcct tagaaggata ccaaaacatt taccagctct tatgaaggct
    37321 agtaaagttc aacataaagc agcacttgtc ggctttgatt gggacaatgt agaagatgta
    37381 tttaaaaaaa ttgaagaaga atatcaggaa ttacttgacg aacacagggt cgggaatata
    37441 aaatacataa aggaagagct aggcgattta ttattttcga tagtaaatct agctagattt
    37501 ttagacatag actcaggaga agccttgaac tgtactacag aaaaattcat aaatagattt
    37561 gactttatag aaaaaagtgc aatgaacttg aataaaaaat tagaagacat gacacttgaa
    37621 gagatggatg agttttggaa tcaggctaaa aaacaataaa attgtggaag gaaatttgtc
    37681 aaaaatctag aacttttatc acaaagatag aagtctatat tataaacatt tttaggaggt
    37741 aaatatcgtg aataaagctg aattagtatc aaagatggca gaaaaaagtg gattaacaaa
    37801 gaaggaagca gaagctgcgt taaacgcatt tatgagttct gttcaagatg cactagtaaa
    37861 taatgaaaaa gttcaattag ttggatttgg aacatttgag acaagagaaa gagctgctag
    37921 acaaggaaga aatccaagag atccagagca agttatagat ataccagctt ctaaagcacc
    37981 agttttcaaa gctggaaaag gattaaagga tataataaat ggataattag aaatcttgtg
    38041 aaaaagtgtg gataaatttg ccacactttt tctatagaac caaggaaggt gataattatg
    38101 agattagata aatttttaaa agtatctagg attataaaaa gaagaactgt tgctaaagat
    38161 gcatgtgata aagggattgt cactataaat ggaaaagttg ctaaatcttc ttcagaggtg
    38221 aatattggag atacattaga aatacaattt ggagagaaga agatgaagtt taagataaat
    38281 gaagtaaaag agcatgtatt aaaaaatgat gcaaaagaaa tgtacgaaat tttagaataa
    38341 aattaatagt cctctagtat attaatagta gtattattga aatatttact ggggggatat
    38401 tatggaacag aacataactt tgaaagatag atcaaagctg gttatttcgg gagtcgagca
    38461 catatactct tttaatgaca aaagagtaga gctcaaaact tctgtaggcg agatggtaat
    38521 tgaaggcgaa aacctagata tgagcaagct aagtatagac gaaaatataa ttagcataga
    38581 tggaactata aattctatgg tatatgcaaa gcctaaaaag ccacaagaga gtttcttgaa
    38641 aaaggtattt agatagatga taccttttac tcaagatgta agcatttttt atgctactat
    38701 ttatggtgga atactcatag gggtgttgtt tgatttttat agagggctaa gagggaattt
    38761 taaattcatc aattattttg ctataatctt tgatgtattg ttttggtttc tagctacggt
    38821 tataatattc gtaacaataa accttacaga attttttgat ttaagatatt atcattttgt
    38881 ggctttgttt attggtttta ttttatacta taacaccata agtaaaattg ttttatcaat
    38941 tatcaataaa ataattcgtt ttgtgagaaa ttcttttaaa aaagttacac attacatagt
    39001 cagtttttta aataatttgt attacgttat aatctattca ctacacctac tatttgatat
    39061 tatattttat ataccaaaca tatttatagc aacaagaaaa agtataaaaa gaaaatcaaa
    39121 taaaaaatta aagaacaaga aaaaatcaaa acctaagaag aaaaaaaata aaactaaaaa
    39181 aagggtgtag gaaaaatgaa cttaagaaaa aaattttcag gacagattat tgttatcagt
    39241 ttatttttag gaatatcaat ttttagtatg atgacaggat ttgtatttga gtatacaaaa
    39301 gctaaggaat ataaaaaaga aatagcaagt ctaaataaac aattaaaaaa gactgaaata
    39361 caaataaaca gcttaaagaa agatgaaaaa tcatatgaag gagacttaga ggatatagca
    39421 agaaaaagat tgaatatggt aaaaccaaat gaaacagttt atgtagacat aaatcgttga
    39481 ttaaaacttg ctaataagtt tacagatgta ttgattttaa aatttaagat ttaataaaaa
    39541 agtcattcag ttaaggatgg ctttttgttt ttattgagga gggaatttca taatgaaagt
    39601 aggagctata gatattggga caaactcaat gagattactg atagcagatt atagagatgg
    39661 gaattttata aacagaaaaa aatttgttaa tacaactaga attggttatg gtgtggatag
    39721 taaaggatat ataaaagaag atgctatcaa aagaaatata aaagcattaa aagaattttc
    39781 tgataagtgt aatgatgaaa agtgtgaaaa aatttattgt atggggacat ctgctcttag
    39841 agacagtaaa aatggagatt actttgttaa attagctaaa gaagaaactg gaatagatgt
    39901 tgatatagtt acgggaatag aagaatcaaa tttgggcttt aagggtgttt tagaaggaat
    39961 aaaaggtgat gatactatta ttgttataga tattggagga ggttcaacag aatttattgt
    40021 aggtgattca gaaggaatta tatttaataa aagtgagaat gtaggtgctc ttaggatgac
    40081 agagaggttt ttgaagcacg atattattga agatgaagag tttgaagcta tgagttcatt
    40141 tatatatggt gagattgaca aaacgttaaa tcatataaaa gacagaggat taaaaactct
    40201 tataggtata ggaggtacga ttacatcatt atcagcaatg aatcaagaat tagagattta
    40261 ctctatggaa aaaatacata atagtaaaat acacaaaaaa gaaatagaag aaattctaca
    40321 aaaattaata aagatgacat taagtgataa aaagaactta aaagggctac aagaaaaacg
    40381 tgcggatatt ataactgctg gtgtaaaaat attgaatata ataatggaaa aattagaaat
    40441 agaagaaata accgtaagtg aatacgataa tttggaaggc ttaatatgtc aaaagtcaaa
    40501 aaaaatgtat taatattttt tgatttttaa aaccgattat gacaaaaaaa attactcctc
    40561 tttgttacaa ttcttaatat aaacaaaaga ggaggttttt ttatgcaaag aagtgtagca
    40621 gttgtgaaga ataagactgt aaaaccaaat gtcataaaat taataagcgg atatattgat
    40681 tttactacgg cggcattcgc tgtgatggga tttttactta gtaggtctat tatagtagac
    40741 tctgtagctc ctttaggaat tgcctttttt atatgtgcat caaagattga taagtacaag
    40801 atacctgtat tcatatcaac attattagga atattgttat catctaatga ttttggaaat
    40861 gtagcaaaat atgctgtgtg ccttacaata tttatggcta tgaataaaaa attaaaatta
    40921 tcagattcaa ttgctaaggt tgcatttata ggtgcaataa tactacttcc aatctccatg
    40981 ggacaagctt tcttttcaaa taggtatgta tatggtcttc ttatagcagg agtagaagca
    41041 ataataatgt ttatatctat atatgtgttt tcttatggga taggtttaat ggccaatgtc
    41101 aacaatagga tgtctgtacg gacagaagaa gccatatcaa taagtttact gctaaccttt
    41161 agcataatgg gaataggaga tatatctctg tttggaatat caataaggac cattttatcg
    41221 accgtattga tattgctgac ctcaatagta ggtggagcta ccatgggagc atctagtggt
    41281 gtaattattg gtgtagcatc cattttaaat aatatgacat cagctgttta tatggggata
    41341 tattcttttt caggattaat aagtggagca tttaataaaa taaataaata tttctgtata
    41401 ctaggatata ttctaagttg gacaataata tacctatata cttctggtat tacttctaat
    41461 atgatgcaat taagagatat actattaggt tgtttgatag tactagtatt gccagagaga
    41521 ttatttaata agattgaaaa attaataaaa agtaatgtgg cttcaaatga aatcgtatac
    41581 gattatatta tgagaagcaa aaacctaact aatagcagat tgaacagtat ttataaaact
    41641 tatgatgacc ttgcagatac atttgataag ataagagaaa aagacaaagt gctagaccaa
    41701 agagacatag ctaatgtaat agatatgatt cataatgatg aatgtaaaag ttgtagtatg
    41761 agaagaatgt gctgggagtc aagatttaat catacataca ctatgatata taacattctt
    41821 gaaaaaattg aggagaaagg ggagttaagt ctaaacgata tacctaaaaa ttttagaaag
    41881 gaatgtatga aaccagagtc aatagtgaaa atatcaaatc actattacaa aatgtttgta
    41941 ttagattatg attggagtgt gaagttttca gaaagtagaa agttaatagc aaatcagata
    42001 agaagtattt caaaatccat taaatcctta tctcaagatt tagaaggcga cataatgcta
    42061 gatatagaaa aggaaaaaaa tatatatgaa cagttagaaa gatatgatat aacagttgat
    42121 aaagtaagtt acttaacaaa aagtaacagt gagtttgaaa tatcaataga aaagaaaact
    42181 tgccatgatg gatgtatgtg tgaagataaa attgtaaata taatttcaga cctagtaggg
    42241 gaaaatatgt ctgttagaaa aataggatgt cactgtttag gaggtaaatg caaggcaaca
    42301 ttcgttaaat ctcaaaaata taaggctgtt acagaagttt cagcaatgtc aagggatggg
    42361 catatccttt gtggagacaa ttacacatac atggagataa atgatggtaa atacatgatg
    42421 gctataagtg atggtatggg aaaaggtaaa aaagcatatg aagaatcttc tgccactatt
    42481 gatatactgg aaaaaatgat agatgcaaaa attaaagatg agatagtcat tgatactata
    42541 aataatatgt tgttgttaaa gtcttcagaa gagatgtttt caactctgga tttgggaatt
    42601 ttagatttaa aacgaggttg tttagaaact ataaagatgg gagcgtgttc tacttatata
    42661 aaaagagagg atggagaagt tgacttaata tcgtcatcat cattacctgt gggtatatta
    42721 tcagatgtaa aaattgatag aaaaaatgta aaagttaagg aaggagacta tgttataatg
    42781 gtttctgatg gaattgtaga tgcaggaaga aataacaatt taggagacaa ttggttaata
    42841 tacttcttaa agaatataga gactacaaat ccaaaggaaa tttcaaattt aatattggat
    42901 agagcattag agttacaagc gttacaaatc gaagatgaca tgactgtttt agttacgaaa
    42961 atatgtacaa aataaggttt acaagattat gtgtataaga tgtggataac ttgtgtataa
    43021 tatgttaata gacttttgaa aaaagtatag ttattaacaa aaataacata taaatcaaca
    43081 aaatttgttg ataagtatgg cagaaatgtt aataaaatgt aaaaaaagaa aaaacaaaag
    43141 ctattatcaa aagagattca tgttgaaata actaaatttc taagtaaaga aaacctgtga
    43201 atatgttgat tattatgtgg ataactatta attcgatatt tatttatata gtatgaataa
    43261 ttgaataata tatctaaaaa aagccatcct attattaagt aaacttattg aaaggatggc
    43321 tttttatgaa tagaaaattg tgcaaagcaa taataccttt atttatttct attttttgca
    43381 tgttaataat aagtataaaa gctgaccaag gatatcaaaa gaataatgac agagcaatta
    43441 ctgtcatgaa taataatgat actgaagatg atgctgaaaa aaaacaagaa atagactatg
    43501 aaaaaacatg ttggaatttg caatctttgt ttaaaagtga tgaccaatgg aaaaaagaac
    43561 ttaagagttt tgataaagat actaaagagt tgaaaaatta cataggtaaa gtaacaaaat
    43621 cgccaacaca tttgtctttt gccttaaaca ttaaagaaaa acttgatata agactaaata
    43681 aactttctgc ctatgtaaaa ttaaaacaag atactaataa aaattcgtat aagtacttag
    43741 atatgaatga tagtatgagt aaatcctatg ggaattatat ggggatttgt tcagatttag
    43801 agttagaaat actaaaatta tcaaacaaag attacaagaa aattatttca aataaaaata
    43861 taaataggaa atatggtgca tatttaagag atataagaag gaataaagta cattatttgg
    43921 atgataaatc agaagatatt ctaagcaaag tatcttcaat aagtagtctt ccaagccaag
    43981 tgtatgaatt atttagaaat atggatagaa aaactaattt gacaccagct caatatgcga
    44041 gtgaattaga gtcatcagat agagaaaata gaaaaaaagc ttatcaagat gaacttataa
    44101 cttacaatga taatataaat acaattgcag gtttaattac tggtcaagtt aaaaagaata
    44161 ttttttattc tcaggaaaga ggttatgagt cttctttaga aatgtacctg gaatctgata
    44221 atgtagatga aaaagtatac aataatttga taaggactgt aaatagtaat atggacagtc
    44281 tacacaaata tatagattta agaaaaaaag tattaaaatt agataaggtt cattcatatg
    44341 atatgtttgt gcctatggtt aaggctgttg ataataatat taattatgaa aaggcacaaa
    44401 gtatagtata ttcagcgttg tcacctttag gaaaagaata caatgatgta gtatataaag
    44461 ctttcaatga aaaatggata gatgtttatt cacatgaaaa taaagtaagt ggtggatact
    44521 gcttatttgt ttatgaaaat catccatata tacttttaaa ttataataat tcattaggtt
    44581 cagtttcgac tttatcacat gagcttggac atgctgtata tgaatattta agttctaaga
    44641 atcagaatta ttttaattca aatccgtcta tatttactca cgaagttgca tctacaacga
    44701 atgaagcatt actttacgag atgcttataa aagaagctaa aggcaatgat gaaaaagctt
    44761 attatataac acagtattta gacttgataa aagatactct ttatacacaa actatgtatg
    44821 ctgaatttga aaaaactata cacgagttag tagaaaaagg aaaaagtgta aacgcattag
    44881 tattaaatga tgtttgggga caacttctta aaaaatatta tggacaaagc tatgaattag
    44941 accaattatc taaggtcgga tgggctagaa tcccacattt ttataatagt ttctatgtct
    45001 ataaatatgc tacaggttgt agtgcaggag taagttttgc ccaagacatc ctaaagaatg
    45061 gttcagataa ctatatgagc tttcttaaaa agggaagttc agattatcca ataaacttgt
    45121 taaaatcaag tggcataaat ttaactaata caaaacccat agaaaataca atcaaaaaat
    45181 ttgatagttt agtagcagaa cttgaaaagc tattataaac attattgtaa tccacatggt
    45241 aaatatatcg aattaatgat aaaatatact aaaagtttaa agtcctatca agataggact
    45301 ttttatttat taattagtgg gagatgattc tatgcaagtt aaagttaaaa aggctgtaat
    45361 accagcagca ggtttaggaa ctagattttt gccagctaca aaggcacaac caaaagagat
    45421 gttaccaata gttgataagc caactctaca atacataata gaagaagctg tggcctcagg
    45481 aatagaggaa atactaataa taacaggtag aaataaaaaa tctattgaag accactttga
    45541 taaatctgta gaattggaac ttgatttaga gaaaaaaggt aagaaagaat tgttggaggt
    45601 agtacaaaac atatctaaca tgattaacat acattatata agacaaaaag agccaaaagg
    45661 gttgggagat gcaatttatt gtgcgaggca ttttataggt gatgagccgt ttgcagtaat
    45721 gttaggggat gatattgtag ataatgatgt accttgtcta aaacaactaa cagatgcata
    45781 tgaggaatat agaactacaa tattgggtgt tcaaaaggta aatcaagaag atactaataa
    45841 atatgggata atagaagcaa aaaatataga aggtagagtt tacaaggtaa aagacatggt
    45901 agaaaagcca gagtcaggaa aagctccttc aaatattgct attttaggta gatatataat
    45961 aactcctgaa atctttgata ttcttaagga tttaccacca ggtaaaggtg gagaggtaca
    46021 acttacagat gctttaaaaa tattatctaa gaaagaagct atgtatgcat acaattttga
    46081 aggcaagaga tatgatgttg gggacaagct aggattttta gaagctactg ttgactttgc
    46141 tctaaagaaa gaagacctta aggaagattt tataaaatat ttgaaacatg tatgtagtga
    46201 atttgataaa aataacaatg tgttatacaa taatacagat gaaaaaatag atttaattga
    46261 agtcaatgaa gctagagaac ttgaaattca aaaataatta atgaaaggtg atgtatggtg
    46321 gcaaaatcaa gtcagaaaag aattaataga ataatagctg ttgggattgt gggaatcctc
    46381 gtaataacga gtataacagc tgttttgagt atgattgcaa ctatgtaata agataaatga
    46441 cagcagttga tatacataaa tataaaaacc atatataact tgatgtagtc aaaaacttac
    46501 aatattttag ttatatatgg tttttagaat ttataaatgt aaaaaaaaat ttttataata
    46561 gttgcatatt ttaaaggaaa atattattat aagatataaa tatagtgata aatttataaa
    46621 aacgtgctaa gattggactt gaaatacagt aatatagtag gtaataatgt tttaaatttt
    46681 atttattgac cataatatat aaataatgat attattagac ttaactgttt aaataaaact
    46741 tttctttttc atgccttcaa tttccaaaat aagtatgtat ataaatgaat atatagatga
    46801 ggaggatttt tttgatttta gatgataaag tattagaagg attaggtagt aagaccttag
    46861 ttgagctacg agagatagct aaagaattaa aaataaaatc aattactaca tataagaaaa
    46921 atgagttaat tgagattata aattccaaaa gtaaaaatga ggaaaaagta aacgaagaga
    46981 ctaaagaaaa aaatgaagat attaaagaag acataaagaa tagaaaagaa aatatagagc
    47041 gtgaaagtat agaaaaagaa gttgaatata agagccctac caaatcatac aacaagaaag
    47101 aaatagatac ccaaaatatt tcccaaaacc aagaacatag aaatcaaaga aatgactata
    47161 ataagacaac gagtgtaaat gatagtaata agtcatcaaa tatatctaat agaatggtca
    47221 gaaataataa taaaaactat tatatgccta aacaagttga tgaatctaag atagtagatg
    47281 aatttaatac atcaaaagag gatgaagtag taggtgtact tgaaattctg ccagatggat
    47341 ttggtttttt aagaggttca aattatttgt ctactgaagg agatgtttac gtttcaccat
    47401 ctcaaataag aagatttaat atgaaaacag gagacaagat taagggaata actagacatc
    47461 caaaaagtgg agagaaattt agagcacttt tatatgttca aaaaataaat gatgaaaacc
    47521 ctgacacagc tatacaaaga aatgcgtttg agacattaac accaatattt ccagaagaac
    47581 gacttacact ggaaactaat agaaatgaaa tagctacaag aattatagat ttaatttcac
    47641 ctataggaaa aggacaaaga ggattgatag tagcacctcc aaaagctggg aaaacagttc
    47701 ttttaaaaag cgttgcaaat agcatagcta aaaatcatcc aaatgttgaa cttatagtac
    47761 tccttataga tgaaagacca gaggaagtta ctgatatgaa agaatctatt gaaggagacg
    47821 ttatatattc tacttttgac caagtatcaa gtcatcatgt caaggttgca gagatggtgt
    47881 taaatagagc tcaaagatta gttgaacatg gtaaagatgt agtaatttta ctagatagta
    47941 taactagatt ggcaagggca tataacctta caatatctcc tacaggtaga actttatctg
    48001 gaggtattga ccctggtgca cttcatggac ctaaaaagtt ctttggtgca gcaaggaata
    48061 ttagacaggg tggttctttg acaatattag gaacagcatt ggttgaaact ggttctagga
    48121 tggatgatgt aatatttgag gaatttaaag gtacaggaaa tatggagcta catttagata
    48181 gaaaattagc agaaaaaaga atattccctg caatagatat ttacaagtct ggtacaagaa
    48241 gagatgattt attgcttgat gatgaagaaa agactgctct ttggagatta agaagagaaa
    48301 tgagtaataa ttcagtaatg gaaattacag ataaagtcat tgaacttata aagagaacta
    48361 aggacaataa agagtttgta aaatcaataa agaatttgta aaatcaataa tacaaatgca
    48421 aatatggatt atataaacta atagaaaaat cacttgaatg gcataataag ttgtgctata
    48481 ataatgtagt tgataatgta aaaagaaagt taatataatt aattttttta aataaagaga
    48541 agaggtgact ataatgcaaa aggaaataca accaaaatac aatccagttg aagtgcgttg
    48601 tgcttgtggg aacacatttg ttgctggttc aactaaggac gaaataaaag ttgaaatatg
    48661 ttcagagtgc catccattct atacaggaaa acaaaagaac atagaaaaag gtggaagaat
    48721 cgacaaattc aagaaaagat tcaaaatgga ctaattttta taaaagtgga gttggaggaa
    48781 gccaactcct attttattac aaaaaaacat tcatattcta ctttaaattg tctttttgta
    48841 ttataataat aaatatgtaa aattgagtat tctaaataca ataaaatgag gtgagtggat
    48901 ggcaaaacaa gctgtaggtg ggcaagctgt tattgaaggt gtaatgatgc agtcaaaaga
    48961 caaaagagcg gtagctgtta gaaaaagtga cggagaaata gcactaaaag aagatagaat
    49021 aaaaagttgg gtaagagata agaatataga taagatacct tttgttagag gttcttttgt
    49081 tatgatagat acaatgattc aaggcataaa aagcttaaac ttttcttcag agttttttat
    49141 ggaagaagct gaagaggata aatttgattt atttataaag aaaattttta aagataaggc
    49201 aaatgatata attataatat tttcattggt catagcaatg ttactttctg ctggattatt
    49261 catttttata cctacattgg taggaggagc tttttctaaa gtaatgccta atgattttat
    49321 gcttaattta atagaaggaa tcataaggat agctatatta tttgcatata tagtattgat
    49381 ttcacgaagt aaggatatag aaagagtctt tcaatatcat ggagcagaac ataaatctat
    49441 atattgctat gaaaatgatt tagaacttac agtagagaat gctagaaaat ttaaaagatt
    49501 acatcctaga tgtggtacaa atttcctatt tatagttatg gctgtatcaa taatattgtt
    49561 tgcatttttt ggttggccaa atccaattct tagaatattt atgagaatta tatgtgtgcc
    49621 tatagtagca ggtttatctt atgaggtaat tagagttctt gggaaatatg ataatggatt
    49681 tactaaaatt attgcatatc caggaatgat gttgcaatat tttacaacaa aagagcctga
    49741 tgatgagcaa ttagaggttg ctttggaagc attgaaagca gtagtagatt agagaaaagg
    49801 atatttgtta tgacgataaa agatataatt attaaatatt cagataaact aaaagatata
    49861 agtgatactc caagattaga tacagagtta ttgttacaaa aaacattggg tgttgataga
    49921 ttatatattc acttaaatct gaataaagag ctaactgaag aacaaaaaac aaaatttatg
    49981 ggttttgcag aagaaagatt aaatggaaga ccaatagcat atatagttga aaatagggag
    50041 tttatgggat tagatttttt tgttaaggaa ggcgtattga ttccaagacc agatacagaa
    50101 actttagtag aagaaataat tgaaatatgt agagaaaaaa aagatgtaag tatattagat
    50161 ataggaactg gttctggagc tattacaata agtttagcaa agtacattga aaattctaaa
    50221 atcatgtctt ttgatatatc agagacagca ttagaaatag ctaaaaaaaa tgctattata
    50281 aatgaagttg gtgaaaaaat aaagtatatt aattctgatt tatttacagc aataagtgat
    50341 agtaacataa aattcgatat catagtttca aatccaccat atattaaaaa acaagatata
    50401 gaaactctgc acaaacaagt taaagattat gaaccatata atgctcttga aggtggagaa
    50461 gatggattag acttttatag aagaataaca gaacaaggta agaaatattt aaataagtgt
    50521 ggaatattag catatgaagt tggtcataat caagctgaag atgtaataaa tattatgaaa
    50581 agtaatggat ataaaaaaat atacacaaag aaggatattc aaggcattga tagagttgtt
    50641 ataggttaca atatatgata taatcaacaa ttgatatgtt acatttaatc gaggtgaaag
    50701 tatgttaaaa aaattagagg tattggaaga tacatataaa gacttaagtg aaaaaatagg
    50761 tgatccggat gtaataaatg atcaaaaagt ttggcagaaa tatataaaag aacatgctga
    50821 cttggaacct attgttatga aatacagaga gtataaaagc gttttagata gtataaaaga
    50881 atcaaaagaa attttacaag aagagtcaga tgaagaatta agagaattag ctaaaatgga
    50941 gttagctgag atggaagaaa aagtagcacc tctggaggaa gaaataaaaa tactattatt
    51001 gcctaaagac cctaatgatg ataagaacgt tatagttgaa attagaggtg gagctggagg
    51061 agatgaagca gctctatttg ctggtgattt atttagaatg tactcaaggt atgctgaaag
    51121 aagaagatgg aaaatagaat tattgagtgc aagtgatact ggagttggtg gatataaaga
    51181 agtatctttt atgataaaag gtaaaggtgc ttattcaaga ttaaagtatg aatctggagt
    51241 tcatagagtt caaagaatac catcaactga atctggtgga agaatacaca catcaacttc
    51301 tactgttgca gtattaccag aagttgaaga tgtagaggtt gaaataaatc caaatgattt
    51361 aagaatagat gttttccgtt cttcaggtaa tggaggacaa agtgtcaata ctactgactc
    51421 tgctgttagg gtaacacata tacctacagg tgaagtagta tcttgtcaag atggaaaatc
    51481 acaattaaaa aataaagagc aagctttaaa aatattaaaa gctagacttt acgataaagc
    51541 attggcagaa caacacaaag atatagcagc agaaagaaaa agccaagtcg gaacaggaga
    51601 caggtcagaa agaataagaa catataattt ccctcaaggt agaataagtg accatagaat
    51661 aaatttaact ttatataagt tagatgcatt cttagatgga gatatagatg aaatgataga
    51721 tgctttaatt actgttgacc aaacagaaaa aatgactgca atataagtaa tgttttaaag
    51781 aagctgtttc ttcctagaaa ttattttcta ttttgaaaca gctattttgc attttaggtc
    51841 atgttatcat attctattgt atataattgt tatatacaag tataggaggg ttatatgata
    51901 ctaaaagtaa caataattgg gcttttggca ggtgttatag ggacaggatt aggaggagta
    51961 atatcagcca tttttaaaag agaggtcgat aaatatctaa gtttttttat gggattatct
    52021 ggtggaataa tgttggctgt agtggtgttt gatttaatga aagaatccat ggacaaaatg
    52081 gggataatca atacagttat atttacattt gttggagctc tcataactat gtacataaaa
    52141 acaaaattag atgtgtctgg aaatatggca tctggatatc ttatattcat aagtatatta
    52201 ctacataatt taccagaagg actagccatt ggttcttctt ttatgtcaac tgaaagttta
    52261 ggtattacac ttgctatagt tataggtcta cataatatac cagaagggtt agctatggca
    52321 ttaggcttag tttgcaataa aatgaaacta agtaaagtta ttttgtttac tgtaatagct
    52381 ggattaccta tgggtctagg aagtttttta ggtgtttatt ttggaggagt atttacttct
    52441 cttataggag tatttcttgc tacagcagga ggtactatga tgtatgttgt attggaagaa
    52501 atttttccac attcaaaaag tgtgtattgt ataattggtt tcttgttagg tacaatgatt
    52561 gtaaattata tataaaatta aaatgaaagc aggcatacaa aagatgaaaa ctattataag
    52621 taatattgat ataaataata ttgattatga ggaaataaaa atacaagcaa aacttcttag
    52681 agaaggtaaa acagttattt ttccaactga gacagtatat gggttgggtg caaatgcatt
    52741 agatgaaaat gcagtaaaga agatttatga agctaaagga agacctagtg acaatccatt
    52801 gatagtccat atatatgaaa aagaagaagt atatgactta gccaaagata taagtgataa
    52861 agctaaactt gttatagaaa agctttggcc aggacctata acaattatat taaataaaaa
    52921 agatataatt ccatacaaaa ctagtggagg gcttgataca gttgcaataa ggatgccttc
    52981 taatgttatt gccagggcaa taataaaaga agctggtata cctattgcag ctccttctgc
    53041 aaatatctca ggtcgcccat ctccaacaaa agcaaaacat gtatatgaag agatgaatgg
    53101 aagagtggat ggaataattt taggaggaga cagtaatttt ggattggagt caactgtatt
    53161 ggatttaaca gaagagactc ctatgatact gagaccagga agtataacta aagaagtttt
    53221 agaggaactt cttggagaag taaagcttga cccatcatta agtaaaaaag aagataatca
    53281 aaaagctaaa gctcctggaa tgaaatataa acattattct ccaaatgcag atgtatatat
    53341 aatatctgga aaaagagaaa atgtagcaac aaagattaat gatatgataa aatctaatag
    53401 agacaaaggt ctaaagacag gtgtaatgtg tattagtaga aaccgagagt tttatgatgg
    53461 agaagtaatc gatttaggaa actctcttga agaagttgca agtaatctgt ttgatgctct
    53521 tatagaaatg gacaaaaagg gtgttgatgt catatattca gaagagttcc ctaagactgg
    53581 agttggacaa gcaattatga atagactact gaagtcagca ggatacaaag tgataaaggc
    53641 ttaagaggga gttgtaaaat atgaatatac ttattgtgtg tacaggtaat acttgtagaa
    53701 gtcctatggc tgaggctata ctaaggaaag caattaaaga atctggaaga agtattgaag
    53761 aatattctat atcatcagca ggtatatcta cagcaaatgg aatgggtgct tctgaaaatt
    53821 ctatagaagt actaaaagaa atcggtatag acttatcaaa tcatagaagt aaagtaatta
    53881 ctaaaaaatt aattgatgaa tcagatataa tactaacaat gactaaatct cataaagaaa
    53941 ttttagtaca agcagttcca aaatgcaaag aaaaagtgta tacttttaaa gggtttgcaa
    54001 ataaaaatga ggaagatata tctgacccat ttggtggaaa tttggatatt tatagaagca
    54061 ctatgagaga aataatgtat tctgtcaacg aaatagtaaa aaaaatatga aacggaggat
    54121 aaacaaatga agataggatt aggctgtgac catggaggat ataatttaaa aaaagaaata
    54181 ataagctatt tagaaagtaa aggtatagag tgtgtagatt atggaactaa taatgctaca
    54241 gattctgttg attatcctgt ttatggagaa atagttgcaa attcagttat aaataaagaa
    54301 gtagattatg gaatattatg ttgtggtaca gggataggaa tatctttagc tgctaataag
    54361 gttcctggga taagatgtgc agttgtatca gatgtatttt ctgcaaaaat gtcaaaagca
    54421 cataatgatg caaatatgtt atcattaggc gaaagagttt taggaaaagg tctagcactt
    54481 gagatagttg aagcatggat taatacggat tttgaaggtg atagacatgc tagaagagta
    54541 aacatgatta aatcaataga agaaaaacat aataaataat aaaaattaca gttttatcag
    54601 gaggaattat caaaatgagc aaagtggtag agacaaacca tccattaata caacataaat
    54661 taactttaat gagagataaa aatactggtt ctaaagactt tagagagctt ttaactgaaa
    54721 ttgcaatgtt aatggggtat gaaataacaa aagatatacc attaaaagat gtagaaatag
    54781 aaactcctat tcaaaaaaca tcatctaaag tagttgcagg taaaaaatta gcaataattc
    54841 caattttaag agcaggtctt ggaatggtag atggattagt gagcttaatg ccagcagcaa
    54901 aagttggaca tgtaggacta tatagagacc cagaaacatt aaaaccagtt gaatattatt
    54961 gcaagcttcc tcaagacata ggagaaagag atataatagt agttgaccct atgcttgcaa
    55021 cagggggttc tgctgtagca gctatagatt tacttaagtc taaaggagct aaaagtataa
    55081 aattagctaa tttagtagct gcaccagaag gtatagcaga agtgcaaaaa tatcatgatg
    55141 atgttgatat atatgttgca tcagttgacg aaagattaaa tgaacatgga tatataattc
    55201 ctggtcttgg tgatgctgga gatagattgt ttggaactaa ataaaaataa ataactataa
    55261 atatttatta gggactttct tatatagtgt atataatttt atgtaagaag gttctttttt
    55321 atatattttt tctcaaaata ggaattattg aattgataaa tttctcgaat tgagaataaa
    55381 aagtttttac caattaaaaa agattgctta ttttacaaga ttttatagaa tagtagtcta
    55441 ttttaatgtg gcatgttagt tgcttacgat taagattata ggtattaagt gtattagtta
    55501 aattgagaat tgaatattaa taatattatt gggagtggta atacatgaag aattgtatag
    55561 tagtaagagg ggctggagat ttagcaacag cagttattca taaattacac aacagtggtt
    55621 ttaaagtagt tgcgttagaa atagaagagc cattagctat aagaaggcaa atttcttttt
    55681 gtgaggctgt ttatgaaaaa aaagtaactg ttgaaggtgt agaatgtata ttttgcaata
    55741 ctgaaaaaga aatagaagaa gttttatctc aacataaagt agccttgatt atagacccaa
    55801 aaggagaaaa attatctaaa ttaaatccaa tcgttttggt tgatggaata ctcgctaaga
    55861 aaaacttagg tacaactaaa gatatggcac cacttacaat agctttaggt cctggatttt
    55921 gtgcaggaga agatgtagat gttgttatag agacaatgag aggtcataat ctaggaagaa
    55981 taataactaa tggtagagca gaaaaaaata caggagtacc aggagcaata aaaggttttt
    56041 ctaaagagag agttatgcat tcacagtatg atggcataat ggaaaatgta tgtaagatag
    56101 gtgatatagt tgaaaaggga gacctacttt gctatattaa taattatgaa aaaaaacatg
    56161 aagttagagc aacaatatct ggagtcctta gaggtttatt aaaggatgga gcaaaggtaa
    56221 acaaaaattt aaaaatacta gacatagacc ctagaaaaga agaagttaag aatgctttta
    56281 caatatcaga taaagctaga tgtatagctg gaggggtttt agaagccata ctaaacaatg
    56341 ggatttatcc ccaaaattaa tttatattag ataaagctat taatatttta ttaagatgat
    56401 ataataaatt taatagatta aagagagcat atgacgggta tttaaaattt aaatggtaaa
    56461 aatgctcttt ttttattgga cattcaatta acatgtgtat ataatataaa aataagtatt
    56521 tatttaggtt gaaatctaat aaatatataa taccagtaca taagcaaaga ggagaaaagt
    56581 aaattatgag accatcatgg gatgaatact tcatggaaat tgcagaagta gtgaaaaagc
    56641 gttcaacttg tattagaaga caagtaggag ctgtaattgt aagagacaaa caaatattaa
    56701 caactggata taatggttct ccgagaaatc ttgaacactg tgaaaatatt ggatgtaaga
    56761 ggcaggaatt aaatattccc tcaggagaaa ggcatgaatt atgcagagca ttacatgctg
    56821 aacaaaatgc gataatacaa gctgctcata atggtattag tgtagatggg gcaactttgt
    56881 atgttactac aagaccttgt gttttgtgtg caaaaatgtg cataaatgca ggtatattga
    56941 aaattgttta tgaaggtgat tatccagatg atatgtctac agaattatta aaagaagcag
    57001 gaatagaact cataaaattt tagtggataa aatttataag acaataaatg tcaaattatg
    57061 acactgaaat acaatggtat caaatgatta aaagaaagta tttgaaaaat catcagatta
    57121 gtagtaagat atattttata attaaataaa tttatgagat agattaatta catgttattt
    57181 attttataat aatgagggat actatttatg agtaaaagaa atacacatac agaaattgca
    57241 aagttgttta gtttaataag ccaaatagga ttgatgattg taatatcaat attaggatgt
    57301 actttcttag gaaaattttt agatagtaaa ttgaatacta ctccagttct gacaataata
    57361 tttttattgt tgggtgttgg aggtgctttt agtggtgttt ataaaactct aatagtatat
    57421 acacgaagga agtgatatat tggatataaa attacttaac caggtaaggt cagtaacaaa
    57481 aggtatagta atatttgatg tagttttaat tgtgctaatg ttgataacat caacattaag
    57541 taaaccagta ttattaggac ttatatttgg ctctataata gctatgttaa attttagatt
    57601 attggcgata tcactggaaa aatcagtgac atttcctgct gggaaggcac aagcttatgc
    57661 atcagcacaa tatgctgtta gattatttat aatggcagtt gttctaattg catcagctac
    57721 agtaccacat ttaaatataa tcggaacagc attgggatta ttgagcacaa agtttgtcat
    57781 attatcaaaa aactttataa ataaattaaa gaggaaggag gcataagtat gaatcaagcg
    57841 cattattatc tcataggaaa ctttaagatt agtgaaacaa tactagtaag ttggggaata
    57901 atggcctttt tagcgatagc atcttatttt ttgactagaa acttaaaaaa agttccaact
    57961 agtaaagttc aaatattctt agaatttgca gtaggtggtc ttgctaaaat ggttacagat
    58021 gttatgggta aaaatacagt aaaaagaatg ccatatatga taccatacat aggaagcttg
    58081 tttttatttt tcgcatgttc aaatttaagc ccattacttg gattaaggtc accaacaacc
    58141 gacttagaca caactgctgc atgggcaatg attacatttt ttatgattta ttttgcagga
    58201 gttaaattta aggggttatc atactttaaa gaattggctc aaccacttgc cataatgtta
    58261 ccaatcaata ttataggtga gttagctaga ccaatatcat taagtttccg tccatttggg
    58321 aatattttag gtggaacaat aataatgggt ctgttttatc aacttttagg ttttatatct
    58381 agtttaatac caggattatc aataccaatt ggagaaatat taataccagt cccattacac
    58441 ttgtactttg acttatttgc aggattgtta caagcatata tatttataat gttaacaatg
    58501 gtatttatat caagtgcagg agagttagat taagaaatca acttatatta agataaaaat
    58561 aaaataattt aaaaaattaa agttttaagg aggaaataat tatggaaaca gcaatagtag
    58621 cggcagcatc agcaatagga gcagggatag cagttgcaac aggtataggt gcaggtatag
    58681 gtcaaggtat agcagcagct aaagctgcag aagcagtagg aaaccaacca gaagctaaag
    58741 gtgatataac atcaacttta ttactaggag ttgctatagc agagtcttca gcaatatatg
    58801 gtttggttat atcaataata cttctattcg taaatccatt ctttaaatac ttaggaatgt
    58861 agatttagtc cttaaatatt ttgagatagg tttataggta aatcatctca aacttataat
    58921 catgagatga actttatcat ctcgtgatta taagtagtta tgatttatgg aaaggaggat
    58981 aattatggaa aaagcactag taggtataac gtgggaattt gtgttccaaa ttgttaatac
    59041 ttttataata tttttacttc tgagaaagct tttattcaag ccagtattaa atataattga
    59101 atcaagagaa aatgatatta aaagtgattt ggcagaaggc gaaaaagcaa aaaatgaagg
    59161 tttagctctg aaaaaagaat atgagtcaaa aatcaatttt gccaaggatg aaggtcaaga
    59221 aatcataaaa caagcaacta taagagcaga gcagaaatca gatgatatag taaatactgc
    59281 aaagaaagat gctcttgata taaaagaaaa agcaaacaaa gatatagaac aagaaagaca
    59341 aaaggttata aatgaaatca aaaatgatat atctaacata gcactacttg cagcatctaa
    59401 agttattgaa aaagatttag ataaatctaa acatgaagaa ttgatagaaa actttataaa
    59461 agaggtaggg gaagctaaat gataaatgta atagcaaata gatatgccga agccctattt
    59521 caattaggag aagaagaaaa ttcaactgat gtcttattca aagaactaga aaaagtagta
    59581 gatatgatga ctaaagttag taaagatttc tacaaagttt taaaatcacc attagtttca
    59641 aaatcagaaa agaaaaactt ggtagaaatt atttttagta aggaagtaag ttctaacata
    59701 aaaaactttt taaaagtttt agtagataaa gatagaatat catatttaga agatatcgaa
    59761 ttagcttata aagaattatt aaataagaaa aacaatgtaa ttgatggagt agcaataagt
    59821 gctattccaa tgagtgaaac agatattaaa gaacttgaag tcaaactttc aaataaatac
    59881 aacaaaaatg ttactataga aaatgttgtt gacaagacta tactaggggg agttctagtt
    59941 agaattggaa atgaacaaat agatggcact gtgaaaactc gtcttgataa gatgaaagaa
    60001 aagctatctg aagtaatatc ttaagaatgg cggtgaaccc atgaacttaa aacctgaaga
    60061 aataagttct ataattaaac agcaaataaa aaattatgag aataaagttg agttgacaga
    60121 tacaggtagt gttttaacag ttggggatgg tatagcgagt gtatatggat tagaaaaagc
    60181 tatgtctggt gagttgctag agttcccagg tgaaatatat ggtatggcac ttaacttgga
    60241 agaagaagta gttggagctg ttatccttgg agatgacagt gaaataaaag aaggcgatat
    60301 agttaagaga actggaagaa tagttgaagt tccagttgga gaagctttaa taggtagagt
    60361 tgtaaactca ttaggtcagc caatagatgg aaaaggacct atagcgtaca caaaaactag
    60421 accagttgaa tctgaggcac ctggtataat agatagaagg tctgtttatg aaccattaca
    60481 aacaggaatc aaatcaatag attcaatgat accaattggt agaggtcaaa gagagttaat
    60541 aataggtgac agacaaactg gtaaaacatc tatagttatt gacactatac taaatcaaaa
    60601 aggaaaagat gttatctgta tatatgtagc aattggacaa aaacgttcta ctatagctca
    60661 attagttagc tctttagaaa aaggtggagc tttggattat acaatagtag tttcagcaac
    60721 agcatctgaa tcagcaccac ttcaatatat agcaccatat gctggagcag ctatgggtga
    60781 agaatttatg tacaatggga aacatgtact tatagtgtat gatgatttaa gtaaacaagc
    60841 tgttgcatat agagaaatgt cattgttact tagaagacca ccaggacgtg aggcatatcc
    60901 tggagatgta ttctatttac attcaagatt acttgaaaga gcagctaagt tatctgatga
    60961 attgggtgga ggttcaatga ctgccttgcc tataatagaa actcaagctg gtgacgtttc
    61021 tgcatatata ccaacaaatg ttatatctat aacagatggt caaatatact tacaaccaga
    61081 gttattctat tcaggtgtaa gaccagcagt tgaccctggt atatcagtat caagggttgg
    61141 tggttctgcg caaattaaag caatgaaaaa agttgcagga acattaaaac ttgcatattc
    61201 acaatataga gaacttgcag cattctcaca gtttggttct gacttagatg aagatactaa
    61261 aaagagactt gctcaaggtg aaagaatcgt tgagatatta aaacaaggtg agcatcaacc
    61321 aataaaagtt gaaaatcaag ttatgataat atatgctgtt ataaataatc atttagagga
    61381 tattccgata gataatatag ctagatttga atcagaattg tatgcatttg tagataataa
    61441 ttatccggaa atatcaagaa aaatattagg tggagaagac ttcactcatg atttaacaga
    61501 tgcaataaat gaatttaaag aaaagtttgt tgttgaagta taatcctttt taaaaaagga
    61561 ggtaaattaa ttggcaggag ctggaatcaa agcaatcaaa acacgtatgg gaactgttga
    61621 aagtacaaag caaataacta aagcaatgga attagttgca tcttctaagc ttagaaaagc
    61681 aaaagaaaag gctgaagatt caagagaata ttttaatact ttatacaata cagtaaaaga
    61741 tattgcccaa acaacacgtg gaattagaaa tgaattttta aaagaaagag aagtaaaaaa
    61801 taaatgctat gtagtagtag ctggagacag aggacttgca ggtggataca attctaatgt
    61861 attaaaggaa tctgtctctc atatgaaagg taaaaaggaa acagtgatta ctgttggaaa
    61921 gaaagcacat gaattttttt ctaaaagaaa ctacaatata gcaaaagata taaatagtgt
    61981 tgaagcttgt ggatatgaag atgcgacaga aatttcacaa actattattg acctgtataa
    62041 aaaaggtgaa gtagatgaag tttatattgc ttatacaaaa tttaaatctg ctttatcaca
    62101 agaagttgaa attttgaaat tgcttccatt agtctttgaa aaagatacag acgaacaagg
    62161 aatggcagta aaagaagaaa aacaaaatag atcaaaagta caatatatac catcagcaga
    62221 agcagtttta agttatattg tacctcagtt tatatcaggt acagtatatg gtggaatttt
    62281 ggaatctttt gcatcagagc aaggtgctag aagaactgca atggaatcag ccacagacaa
    62341 tgcgaatgaa atgctatcta atttagagtt aagttataac agagcaagac agtcagctgt
    62401 aactcaagaa ataacagaga tagttggtgg agttgaagct ctaaaatagg gaggttagga
    62461 aatggcaaat gtaggtaaag tagtacagat tgtaggagct gtactagatg ttaagtttga
    62521 tagtgaacaa agcctaccta acttattaaa tgcgttagta ataaagttag gagataaaga
    62581 aatagttgca gaagttgctc aacatatagg tgatgatact gtaagatgta tagctatgag
    62641 tgcaacagac ggacttgtaa gaggtatgga agttgtagat acaggagggc caataagtgt
    62701 tcctgttgga gatgaaactt taggtagaat attcaatgtt cttggaaaac cagttgatgg
    62761 gaaaccagct ccaaaatctg cacctaaatt acctatacat agaccagcac cggcgtatga
    62821 tgaactagaa actacagcag agatactaga aactggtatt aaggtagttg acttactagc
    62881 tccatatcta aaaggtggaa aaataggtct atttggagga gcaggagtag gaaagacagt
    62941 tctaatacaa gagcttataa acaatattgc taagcaacat ggtggtattt ctgtattttc
    63001 aggagtagga gaaagaacaa gagaaggtaa cgacctttat ggcgaaatga gtgagtctgg
    63061 agttataaat aaaacagctc tagtatttgg tcaaatgaat gaaccacctg gagcgagaat
    63121 gagagttgct ttaactggac ttacaatggc agaacatttt agagatgagc aaggacaaga
    63181 cgttttactt ttcgttgata atatattccg tttcacacaa gctggttcag aagtttcagc
    63241 acttctagga cgtatgccat cagctgttgg ttatcagcca acattagcta ctgaaatggg
    63301 tgcacttcaa gagagaataa catcaactaa gaaaggttca ataacatctg ttcaagcagt
    63361 atatgtacca gcagatgact taactgaccc agcaccagct acaacattct ctcacttaga
    63421 tgcaaaaaca gttttatcaa gacaaatatc atcactgggt atatatccag cagttgaccc
    63481 tcttgaatct acatcaagga ttcttgaccc tagtatagta ggaaaagaac attatgaagt
    63541 agctagaggt gtacaatcaa tacttcaaag atataaagaa cttcaagata taattgctat
    63601 acttggtatg gatgaattat ctgatgaaga taagttaata gttgcacgtg ctagaaagat
    63661 acaaagattc ttatctcaat cattcacagt tgctgagcaa tttacaggaa atccaggtca
    63721 atatgttcct gttaaagaga ctgtaagagg atttaaagaa attcttgaag ggaaacatga
    63781 tgacttacca gagtcagcat tcttatttgt aggaacaata gaagacgctg ttagaaaagc
    63841 gaagggaagt atgtagtcat ggccagtgaa ttttcattga aagttgtaac tccaagtaat
    63901 actttttatg atggagatgt tgaaatgata gtagttagaa ctacaaaagg agacaaaggg
    63961 gtgttgaaag gtcatatacc atttgtttca ggtatacctg taggaacttt aaagataaaa
    64021 aaagagggaa gttttaaaga ggcgaaaata gcagaaggat tcattaatgt aactcaagat
    64081 aaaactgtca tattgacaga atctgctgag tggatttaat aaattaattt agagtataaa
    64141 aagtcatttt gaccaaaaaa acttttgggt aaaatggctt ttttgcgttt tgatgttaat
    64201 aaaataacga taaaattgta agtttttagt gataataaat tcacaaaatg tttaaattta
    64261 ctgccaaatt atgttgtaat taataagata aagaagattt aaatagcttt tatgtaagtg
    64321 aaaaagtctt catataagaa atacatataa aatttttata aaatttaaaa aatttgctta
    64381 gttgtgtagt ataataaata atataataga tttgtatata atattcatta caaataattt
    64441 taaaagttac agttttatta taatgattat taaattaaga taagtaacat aaactaaatg
    64501 ataatgtgta ttatcgatag taaatttata aattcattat taattattga aagaagtatt
    64561 ataaattttc tggaggaaca atgaatatat ttgatattgg cgtagatata attgaaattg
    64621 atagaataag aaaggctgta gacaaaaata ataggttttt ggagaagata tttacagata
    64681 gagaaataga gtattttaat tctaagaatt ttaaagcaga aagtatagca ggtaatttcg
    64741 cagccaagga agctataagt aaatctatag gtacaggaat tagagtgttt aattttaaag
    64801 atatagaagt tctcagaaat gaaatgggaa aaccaatagt aaaaacttat aataatctag
    64861 caaaaatgtg catagattat aatgttttag aaataaaagt ttcaatatca cattcaaaag
    64921 attatgctat agcaaatgcg ataaccataa ttaaggacta aggagtgatt acattatgat
    64981 ggataaaaca gcaaaagaaa taatgacaac agatgtaata gtagcaaaac aagatgatag
    65041 tatagcagat gtagctaata tgctaattgc agaaaaaata ggaggtctgc cagttgtaga
    65101 ttcagagaat agagttgttg gaataatttc tgaaacagat attcttaaaa aagaaaaata
    65161 tatagaagca cctctctata taaatctatt acaaggattg attttcttag atgacttaaa
    65221 aaaagttgaa aaagatataa aacaagtagc tgcttataag gttggagaac ttatgtctaa
    65281 agatataata aaggttcatg aagatgataa gtttgatgat gtagctaatg taatgattaa
    65341 aaaatcaata aacagagttc ctgtagtaga tgatgacaac aaacttaaag gtataatttg
    65401 tagatatgat ataataaaag ctttatacaa tgaataaact gaagtaggag gtgtgatgtg
    65461 agaaaaaagt ggaccatagt atgtattatg ttcttggcac tggttataat tgtaatagga
    65521 tgccaaaaac gacagtccac aaaagaagaa gtatacaaag attttcaaaa gcagatttct
    65581 gatatgaatt attacagttg taaagctgaa gtagaagtag ttggaaataa aagcccacac
    65641 aattatgttt taattcatac ttataaaaaa accgataact ataaattaga agtaatttcc
    65701 ccaaaacatt taaaagggaa aagtatagaa tatcaaggtg ataaaatatt agttaaaaat
    65761 ccaaagatta gtgatgtagt ggagttacct aatacaggaa aaaataatca atatcttttt
    65821 gtaggagact ttataaaaaa ctatcttcaa aatgaggaga tgaaagtaaa gttatccaaa
    65881 ggacatttgg ttttagagac ttttattcct ggggataata aatattttaa caagcaagta
    65941 ttatatgtaa atgctgacac aaaaaatcct gaaaaaatgg aagtgttaga taaagaggga
    66001 gtgccaagat ttacagtaaa atacaaagat tttgaataca gaaactaagg gggactaaag
    66061 acatgcaaaa aataacagtg cctacatggg cagagataaa tctagataac ttaagattta
    66121 acttaaataa tattaaaaat ttattagaag aagatattaa gatttgtgga gtaataaaag
    66181 ctgatgcata tggacatggt gcagtagaag ttgcaaaatt gctagaaaaa gaaaaagtag
    66241 attacttagc agtagcaaga actgctgaag gaattgaact tagacaaaat ggcataacac
    66301 ttcctatttt gaacttggga tatactccag acgaagcttt tgaagattct ataaaaaata
    66361 aaataactat gacagtttat tctttagaaa cagcacaaaa gataaatgaa attgcaaaat
    66421 ctttaggaga aaaagcctgt gttcatgtta aaatagactc agggatgact agaataggtt
    66481 tccaacctaa tgaggagtca gtacaggaaa taatagaatt aaataaatta gaatatatag
    66541 atttagaagg tatgtttact cattttgcta cagctgatga agtaagtaaa gagtacactt
    66601 ataaacaagc taataattat aaatttatgt ctgataaatt agatgaggct ggtgtaaaaa
    66661 tagctataaa acatgtatca aacagtgcag ctattatgga ttgccctgat ttaagattaa
    66721 atatggtaag agcaggaata atattatatg gtcattatcc atctgatgat gtatttaaag
    66781 atagattaga attaagacca gccatgaaat taaaatcaaa aatcggacat ataaaacaag
    66841 ttgaaccagg tgtaggaata agttatggac taaaatacac aactacaggt aaagaaacaa
    66901 tagctacagt tccaatagga tacgcagatg gatttactag aatccaaaaa aatccaaagg
    66961 ttcttattaa gggagaagtg tttgatgtag ttggtagaat atgtatggat caaataatgg
    67021 ttagaattga caaagatata gacataaaag ttggagatga ggttatacta tttggagaag
    67081 gcgaagttac agctgagcgt atagctaaag acttaggaac tataaactat gaagtgttat
    67141 gtatgatatc aagaagagtt gaccgtgttt atatggaaaa taatgagctt gtacaaataa
    67201 acagttattt gctaaaataa aaccatgata ttataatctg ggaggcgatt ttgtgtacga
    67261 taagagtaaa gaaaaaatag aagttacttt acctgactct ctgatttctg aagttgatag
    67321 tatagttcag atggagaaca gtaacagaga agattttgca aaagctgctt ttcagttcta
    67381 tataactcag aaaaagaaaa ttcatattaa agaatctatg aaaacgggtt atagagaaat
    67441 gggtcaaatt aatctgtcac tagctgaatt aggtatgaca gttgaagaag tatcttctga
    67501 tgataaaaat gaagggaaga ttgcccaagg tgagtactaa ttgtgaaata aagcgtggtg
    67561 atttatatta cgctgattta agtccagttg ttggttcaga gcagggaggc gttagacctg
    67621 ttctgataat acaaaatgat gtaggtaata aatatagtcc tacagttata gtatcagcta
    67681 taacatctca gattaataaa gccaaattac ctactcatgt agagataagt tccaatgaat
    67741 atgggcttaa taaagactca gtaattcttt tagagcaaat tagaactatc gataaaaaga
    67801 gactgaggga aaaaattggt tgtctagatg aaaatatgat gataaaagta gatgatggac
    67861 ttcaaataag tttaggattg tttacttttt aaagagggat ttgtctttta ttagacaatc
    67921 ccttttttct tttaaatatc tcttaaggtt taattttaat gtactatgtt aataaaatta
    67981 aaaataacgc aaaaaaatag cataaaagtg atataataat atggaattga tataaaatta
    68041 cgaactcaag ggaggtaatt gcatgagaga ccataaagga ttaaatgaga ttgcgcgtat
    68101 cataagaaga gatatagttt caatgataca tagagccaaa tctggacatc caggagggtc
    68161 attatcagta gttgaaatac ttactgcgtt atactttgat gaaatgaatg tggattcttc
    68221 aaatcctaaa atggaggata gagatagatt tgtattatca aaaggacatg ctgcaccagc
    68281 attatatgct acattggctg aaaaaggcta ttttgataag gaagaactga atggattaag
    68341 gaagatagga aggatgttac aaggtcatcc agacatgaaa ggcacgccag gtgtggaaat
    68401 atctactggt tctttaggtc aaggtttttc agttgcatgt ggtatggcta tggcatcaaa
    68461 gttggataat gctccgtgga atgtgtatac tttgcttggt gatggtgagg ttcaagaagg
    68521 aattgtttgg gaagctgcta tgagtgctgc acattataaa cttgataatt taattgcttt
    68581 tttagataat aatggacttc aaatagatgg agatatagaa agcgtaatga gtttaggttc
    68641 aatagttgat aaatttaaag cttttggttg gaatgtaatt gagatagatg gacatgattt
    68701 tgaccaaata tttgcggctc ttgatatagc taaatcaaca gttaaaaaac ctacaatgat
    68761 agttgcaaaa actataaaag gcaaaggaat atcttttatg gaaaatcaag caggatggca
    68821 tgggacagct ccaagtgatg aggagctaga aaaagccttg cttgaattag gaggtgctga
    68881 caatgagtaa aatggcgacg agagaagcat atggtaaagc attagtaaaa ttaggtcaaa
    68941 taaatgacaa tgttgtagta ttagatgcag atttatcaaa gtctacaaaa acacatgatt
    69001 tttacaagtc atttccagat agatttttta atatgggtat agctgaacaa aatttaatag
    69061 gcgctgcttg tggattatca actgctggta aaataccttt tgcaagtact tttgctatgt
    69121 ttgctacggg aagggctttt gagattataa gaaattcagt ttgttatcca aagctaaatg
    69181 taaaaatatg tgctactcat gcaggtctta ctgttggaga ggatggagca tctcatgaaa
    69241 gtgtggaaga tattgctata atgagagcta taccaaatat gacagttctt gttccagctg
    69301 atggagttga aacagaaaaa attatttttg aaatagctaa gtataatgga cctgtttatg
    69361 ttagattagg gagaagttca gtaccagttt tatttgatga agattataaa tttgaaatag
    69421 gaaaaggtac agtcttaaga gaaggaaagg atgtatcaat tatagcttgt ggaattatgg
    69481 taaatgaagc tctattagca caagaaaagt tgcaagaaga aggtataagt gctagagtta
    69541 taaacatgtc ctcgataaaa cctatagaca aagatttaat attagaatca gcaaaagaaa
    69601 cgaatgctat tgtaactgta gaagaacata gcataattgg gggattaggt tctgctgtaa
    69661 gtgaggtagt aggagagtct tgtccaacca ttgtaaagaa agttggaatt aaagatacat
    69721 ttggagaatc tggtactcca aatgaattgt taaaaaaata tgaattaact tgtgatgaca
    69781 taataaagac tgttaaagaa gctattattg caaaaagaat gtaagagata taaattattc
    69841 atgtttgtta ataatataat atatttaaaa gagaggtata aagtgttata ttaatttata
    69901 tctctctttt ttttatatga tatcaatata tcaaaaatgt aataaaatat tcaaattttt
//
LOCUS       DAEMHZ010000004        62742 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000004 DAEMHZ010000000
VERSION     DAEMHZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.004"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(315..836)
                     /locus_tag="KPK72_000247"
     CDS             complement(315..836)
                     /locus_tag="KPK72_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2487498.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNTAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQILIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            1002..1568
                     /locus_tag="KPK72_000248"
     CDS             1002..1568
                     /locus_tag="KPK72_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2487499.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKSTSSYS"
     gene            complement(2090..2959)
                     /gene="gatY"
                     /locus_tag="KPK72_000249"
     CDS             complement(2090..2959)
                     /gene="gatY"
                     /locus_tag="KPK72_000249"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2487500.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(2979..4895)
                     /locus_tag="KPK72_000250"
     CDS             complement(2979..4895)
                     /locus_tag="KPK72_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG2487501.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGSLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(4898..5818)
                     /locus_tag="KPK72_000251"
     CDS             complement(4898..5818)
                     /locus_tag="KPK72_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG2487502.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(5920..6672)
                     /locus_tag="KPK72_000252"
     CDS             complement(5920..6672)
                     /locus_tag="KPK72_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2487503.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(7064..8509)
                     /locus_tag="KPK72_000253"
     CDS             complement(7064..8509)
                     /locus_tag="KPK72_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2487504.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(8531..8884)
                     /locus_tag="KPK72_000254"
     CDS             complement(8531..8884)
                     /locus_tag="KPK72_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2487505.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(8913..10160)
                     /locus_tag="KPK72_000255"
     CDS             complement(8913..10160)
                     /locus_tag="KPK72_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2487506.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(10168..10485)
                     /locus_tag="KPK72_000256"
     CDS             complement(10168..10485)
                     /locus_tag="KPK72_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2487507.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            10727..12640
                     /locus_tag="KPK72_000257"
     CDS             10727..12640
                     /locus_tag="KPK72_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2487508.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     NRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(12763..13917)
                     /locus_tag="KPK72_000258"
     CDS             complement(12763..13917)
                     /locus_tag="KPK72_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG2487509.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(14197..16812)
                     /gene="mngB"
                     /locus_tag="KPK72_000259"
     CDS             complement(14197..16812)
                     /gene="mngB"
                     /locus_tag="KPK72_000259"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2487510.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDRDNNNLDFEVVHKEEIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNILANHTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRVDSFIDFDIVINVPNDKAIIDVVFNINNQVRDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(16956..18881)
                     /gene="mngA"
                     /locus_tag="KPK72_000260"
     CDS             complement(16956..18881)
                     /gene="mngA"
                     /locus_tag="KPK72_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG2487511.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(19011..19736)
                     /locus_tag="KPK72_000261"
     CDS             complement(19011..19736)
                     /locus_tag="KPK72_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2487512.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(19999..20778)
                     /locus_tag="KPK72_000262"
     CDS             complement(19999..20778)
                     /locus_tag="KPK72_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG2487513.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDASISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(20771..22198)
                     /locus_tag="KPK72_000263"
     CDS             complement(20771..22198)
                     /locus_tag="KPK72_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2487514.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            22589..23311
                     /locus_tag="KPK72_000264"
     CDS             22589..23311
                     /locus_tag="KPK72_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487515.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            23508..25175
                     /locus_tag="KPK72_000265"
     CDS             23508..25175
                     /locus_tag="KPK72_000265"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG2487516.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTKENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRAIIENKMEIKPYESIMIYYK"
     gene            complement(25271..26590)
                     /locus_tag="KPK72_000266"
     CDS             complement(25271..26590)
                     /locus_tag="KPK72_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2487517.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(26631..27377)
                     /locus_tag="KPK72_000267"
     CDS             complement(26631..27377)
                     /locus_tag="KPK72_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2487518.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(27621..29630)
                     /locus_tag="KPK72_000268"
     CDS             complement(27621..29630)
                     /locus_tag="KPK72_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2487519.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKNKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(30068..31501)
                     /locus_tag="KPK72_000269"
     CDS             complement(30068..31501)
                     /locus_tag="KPK72_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2487520.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(31517..32941)
                     /gene="ascB"
                     /locus_tag="KPK72_000270"
     CDS             complement(31517..32941)
                     /gene="ascB"
                     /locus_tag="KPK72_000270"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2487521.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDIWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(33028..34944)
                     /locus_tag="KPK72_000271"
     CDS             complement(33028..34944)
                     /locus_tag="KPK72_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2487522.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(35018..35878)
                     /locus_tag="KPK72_000272"
     CDS             complement(35018..35878)
                     /locus_tag="KPK72_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2487523.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(36229..37416)
                     /locus_tag="KPK72_000273"
     CDS             complement(36229..37416)
                     /locus_tag="KPK72_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2487524.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(37430..38821)
                     /locus_tag="KPK72_000274"
     CDS             complement(37430..38821)
                     /locus_tag="KPK72_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG2487525.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(39416..41116)
                     /locus_tag="KPK72_000275"
     CDS             complement(39416..41116)
                     /locus_tag="KPK72_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2487526.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVVYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(41088..42491)
                     /locus_tag="KPK72_000276"
     CDS             complement(41088..42491)
                     /locus_tag="KPK72_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2487527.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(42756..43247)
                     /locus_tag="KPK72_000277"
     CDS             complement(42756..43247)
                     /locus_tag="KPK72_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2487528.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(43273..44085)
                     /locus_tag="KPK72_000278"
     CDS             complement(43273..44085)
                     /locus_tag="KPK72_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2487529.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(44098..45249)
                     /locus_tag="KPK72_000279"
     CDS             complement(44098..45249)
                     /locus_tag="KPK72_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2487530.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            45519..46160
                     /locus_tag="KPK72_000280"
     CDS             45519..46160
                     /locus_tag="KPK72_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG2487531.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            46186..47730
                     /locus_tag="KPK72_000281"
     CDS             46186..47730
                     /locus_tag="KPK72_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2487532.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(47930..48556)
                     /locus_tag="KPK72_000282"
     CDS             complement(47930..48556)
                     /locus_tag="KPK72_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2487533.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(48691..49407)
                     /locus_tag="KPK72_000283"
     CDS             complement(48691..49407)
                     /locus_tag="KPK72_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG2487534.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            49833..51047
                     /locus_tag="KPK72_000284"
     CDS             49833..51047
                     /locus_tag="KPK72_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2487535.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLRTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIISSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            51665..52993
                     /locus_tag="KPK72_000285"
     CDS             51665..52993
                     /locus_tag="KPK72_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487536.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(53131..54321)
                     /locus_tag="KPK72_000286"
     CDS             complement(53131..54321)
                     /locus_tag="KPK72_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2487537.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(54427..55806)
                     /locus_tag="KPK72_000287"
     CDS             complement(54427..55806)
                     /locus_tag="KPK72_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2487538.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIKNNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPIP"
     gene            complement(55837..57045)
                     /locus_tag="KPK72_000288"
     CDS             complement(55837..57045)
                     /locus_tag="KPK72_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487539.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNRDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(58070..59524)
                     /locus_tag="KPK72_000289"
     CDS             complement(58070..59524)
                     /locus_tag="KPK72_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2487540.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDKKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFQNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(59544..61415)
                     /locus_tag="KPK72_000290"
     CDS             complement(59544..61415)
                     /locus_tag="KPK72_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2487541.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVKGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(61405..62265)
                     /locus_tag="KPK72_000291"
     CDS             complement(61405..62265)
                     /locus_tag="KPK72_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2487542.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT        20552 a        11131 c         7025 g        24034 t
ORIGIN      
        1 gatgttgtat caaaatatag ataaatttat ttttcattgc taaaatgatt aaaatatatt
       61 tgatttaata tacttgattt aatataaaaa aagaatgtct cataaactaa aaagttcact
      121 tttagacact cctttatata tattatctac ttcttaattc atttattacc tttctacatt
      181 ttctttcagc tagtggatac atctttataa gtactaatta ctccacttaa aaatggttcc
      241 tgtactgtcc atcagtcata tttttctgcc aaatataatg gtgtttcata atgagacaat
      301 aaatatttat atctctactt atcttctcta agctcgtcaa atccaactat aggtatattt
      361 aaattatatt ctttaagtag cttttctagc tcttctataa aaataccttt cccatccacc
      421 atttgtatat catttatttt atccatcaat tcattgcaac cacttaactc accaaaccaa
      481 cgtttagaac tacatgtctt attataacca caacttggac tgccatctat tgcaatgata
      541 ctttttatat cataatcatt ttttaaatat tcatcaaact gccttacatg aggtattaat
      601 atttgcctac attgttctct aaaaaacaaa ttgtcaaact gttcttttac atgtccccac
      661 ctatttattc catacataat catttctgga catggtagtt gaattattcc atacccttta
      721 tttattaaat ctattgctgt attttgtgct cccttaaatt ctgacaatcc ctctacttta
      781 gaattacaat ttaatataca atgacatact actacaatct ctctatttct tttcataata
      841 atctccttag ttattcttaa ttttaatgat actacatatt ttgataatta tattatttat
      901 ttgttctata tttccaatat tctatataga aatatacaat tagtaatctt ttataatcta
      961 tactatgata aatgtatata tttttttagg aggtattata attggatact aaaaaagtca
     1021 cattttcttc aatgtgcata gttattaata ttgtcttagg tatggttata tctatgataa
     1081 atataccatt attattttta gatacagttg gtactgtact tggagctgtc gtattaggtc
     1141 ctttttgggg tgctcttatt ggtgggtgta caaacttagt tttaggtgtt atatcaggtc
     1201 ctacaaatat accttttgcc cttgtaaata ttgttcttgg tcttattgtt ggatatattt
     1261 ctaagaaaaa aggttttggt tataaagaag ctattattac aggagtaatt ttatctattg
     1321 tttgtccttt gattggtact cctatttcag ttttattgtt tggtggttta agtggaagtg
     1381 gtgctgattt attggttggt tttttagttc agtctggtca gaagatattt actgctgcgt
     1441 tctttccaag gattcttagt aatatagtag acaaaccttt gtcttgtatt atggtggtat
     1501 attttataag taaaatgcct aaaggtttta taaaacaata tagtaaaagt acgtctagtt
     1561 attcttagta aatttgtaca tactatatga ttttttaaat gttataacaa gtttatacaa
     1621 atatatttgt ataatatctt tgctacttta tttttcatta attttagtat tagtgattta
     1681 attatttatt atctgtatta atttaattaa atacaacctc ttaatataaa tttagattct
     1741 aaaattatat caagaggtta tatttttatt ttataatata tttaaagttt tataacaatt
     1801 tatagaatat atgtttttgt tttagctaaa atacctctct aaaaatcatt ttttacaagt
     1861 atttcttata ttaacaaata cattctcatt ttctataaac aactcataca tatcttcaaa
     1921 ctctagtata ccatatccat gagagtcatt atttcttaat tttttaagtg tttttaataa
     1981 tatcttcttt attctatatt tctaatttga cctcttaaat atacaaacta aagtaagata
     2041 tctactatag acatcttact ttagcttcat ttatgaatag gttttattat taacacttca
     2101 ctgtctctat tgcttttcct acacatccac aaacttccat cctactctta acaaaatctt
     2161 taactgactc taaacctttt ttaccatatt tcttagggtc aattacctct gggtcagaat
     2221 taagcacatc cttaacccca ttgctataag caatccttaa ttcagttgca taattaacct
     2281 tgcaaatccc tctttttata gattccttaa taacttcatc tggtattcca gacccaccat
     2341 gtaatactaa tggaactgat acaacatctc taatttcaga taatctatct aaatcaagct
     2401 ttggctctcc cttatataat ccatgtgccg tacctattgc tactgctaaa gatgataccc
     2461 ctgttctctc tacaaactct tttgcttcta atgggtctgt atatgctccc ccatcaccac
     2521 catctaaatc gtcttcctta cctccaactt tacctaattc agcttcaaca ggtatatctg
     2581 aaggcataca tgcatctaca acagatttag atattgaaat attatcttca taactttcat
     2641 gagaaccatc aatcataata gaagtatatc caactctaag tgcctgcata gctagtccaa
     2701 agcttgaacc atggtctaag tgcattgcta ctggaacact tgccttctct gatgctacct
     2761 ttacattcgc taaataataa tctagtcctg catactttat tgttgaaggt gttgtttgca
     2821 atattactgg tgatttaagt tcttcagcag cctctattac tgccataacc atctccatat
     2881 tttcaacatt aaatgcacct actgcataat gcccttcttg tgcatctaat aatatttgtt
     2941 tagttgtcac taatgccata atttctcctc ctatatgttt atactgattt gatttcttcg
     3001 ttatttaatt ttgatactat ttctctactt gttttgctat ttaataactc atttaaaaat
     3061 tcttcattca ttaattttga agatagttga gataatgttt ttaagtgtaa attatcacca
     3121 ttatctgtag ctgctatcat aaatataata tgtgctaagc ttccatcttc tgattcaaag
     3181 tcaaaccctt cttttgatac acctactgct acacgtggta cctttactgc atctgttttt
     3241 gcatgtggta tcgctatccc cataccaaaa cctgtagatg atatttcttc tcttttaagg
     3301 atttcttctt taaacttctt tttatctaaa agaacaccag atttatcaag tattccaatc
     3361 atttcatcaa ttacatctgt tttatttttt cccttaacat ctaaaattac tgtttcttcg
     3421 ctatataaag agatattatt agatacgttt tccgattctg cactttcatt tttatgttca
     3481 tcttttgtta catttgtatt ttctatagta tttttctcat ttactacttt aggtgtaacc
     3541 atcatataaa gtacagcacc tactaatgaa cctacaaata tacaccctac ccataaaagt
     3601 ggcttattga ctaatcctag aaccaaaaac cctccatgtg gagctggtac ttctattttc
     3661 attatataag ttaaaattga tgatattgat gatgcaatca taagtactgg caacactttt
     3721 aatggatttt ttgctgcaaa aggtattgcc ccctctgtta tatgagtact acctagtata
     3781 taattgatta atcctgctgc tctatcttct tctgtaaatc tatttttaaa taaagtagat
     3841 gctattgcta ttaccaatgg tggtgttata caagctgcag atacacctgc cataaataaa
     3901 taatttccct gtcctagaag catagttcca gttacatatg ctgctttatt tactggacct
     3961 cccatatcaa atgctgacat acatcctatt actattccca aaattatagg acttgaattt
     4021 tgtaaccccg atagaaagtt catcatagca ttgttaagtg ctgaacatgg cccaccaagt
     4081 aacaacattg ctacacccac aagacctacg ctgacaatag gaattataaa aatcgattta
     4141 agaccttcta attgtcgtgg taattttctt aacaaatgta caaatagttt aactaagtaa
     4201 cctgctaaaa atcctgatat gattccacct aaaaaccccg aacctgtatc aaatgatatc
     4261 attcctccaa caagtcctgc caacatacca gctcttcccg atatagattg agctataaat
     4321 gctgccaata caggaaccat caagttcata gaatatccac caacagattt taatgtagct
     4381 gctatttgat tatattgttc tgaatttggg tcaaatgaat aaatccccca taaaaatgat
     4441 atagcagtaa gtactccacc tgctactact aaaggtaaca tgtgagatac accattcatt
     4501 aaatctttat acaacttttg accaaacgat aaacttcctt cttctgcaat tttactgctt
     4561 gataagtttc cttttctaat agctactttt ccatcaatac atctttgtat aagttcatta
     4621 gctttatgta taccttcttt gacaggtact tctattaccg gctttccatt gaatctgtcc
     4681 atatcaacat ttttatcaca agcaactatt attgcatctg ccatttcgat atcttttgct
     4741 gttaacttat tttctacacc tatagctcca ttcgtttcaa cttttatgtc acagcctaat
     4801 tccctggctg aattttttaa tgcttcttct gccataaaag tatgagctat tccagttgga
     4861 caacctgtta cccctagaat cattttcttt cccattttta taccaccttt ctgactctta
     4921 tattttttat ataatcttcc acattctcca aattacctat accattactt tcagccacac
     4981 ttgctcctgt tgctgctgac ctttttaatg cttcttcaat atgttctgga ttttccaacc
     5041 atatacttaa aaaagcagct aatgcagaat caccagcaca tgcagagctg actactgtaa
     5101 ctggttgagc actagcataa taaattgatt ttccattata aaaataagaa cctttttctc
     5161 ctaaagttag caatatattt tgagcacctt ttaaatggag taatttaagt acatccttaa
     5221 tatcttcttc atctcttaca ataattccaa atatatcttt gatttcttca tcatttggtt
     5281 ttatcaaata tggattatac tgtagtaact cctttaattt tggtgaactt atatcgagta
     5341 ttgtctttat acttttattt tcacaaattt taaatatatc ctcatagtaa ttatcatcaa
     5401 taccaggagg tagacttcca cttattgata agtaacttat atcttctaat gactgtatct
     5461 tatctagcat atctagtttt tgtttttcgt ttacatatga cccagagttt acaaatttaa
     5521 attcatcact tccatcattt aaaaatatat ttatacgagt tgtatcttct acccaagtag
     5581 gcaatacgtc aaatcctctc ttttcgcttt cttcaactat atattttcct gaaaatcctc
     5641 caaaaaatcc taatatctta gattctatac cataatgacc tagtacaaaa gttacattta
     5701 atccctttcc attaggagaa tatacagcat ttgatgtacg atttactaat tttctcttta
     5761 tcccattagt agatatattc atatctattg caggatttgt agttaatgta tatatcattg
     5821 tcttccccct tgcttgtgct gtgtatgttt tcattataat aaaaaagtct atgaaaatct
     5881 tttcacaaat agaaaatcga ttttcataga cttttcaatt taattttcag aattattata
     5941 tctgaccata taatctataa ttatcctaga tataatatac aagggcaatc tggaagatac
     6001 ttcatattca gaaattgata tatgttcatg tttgtatcct acaatatata aatccgaata
     6061 ctttataagt tttgcacttt cactacaaca gcaagttata accttagctc caactaaatt
     6121 ggcattctgt gctgattcta caagctctat tgtatttcca ttcaatgaaa atataattat
     6181 aagttcgtct ttttttattg atttagattt tattttcatt atgttggggt catttataaa
     6241 aacaacatta aaatcaagta attgcaattt aagactaaac tcttttgcta catattcagt
     6301 aagccctctt gctaaaatat atatcttact agatttattt atagttttta ttatattgat
     6361 tagtgtagtt aaagatattt gttctataac tctctgtgct tctataaagg atttatttat
     6421 tatatccccc atattttgta tatcatcatt ttcatctttt actgttaatc tatacctaag
     6481 ttcattgaat ccatttaaac cacattttct aattgctctt gaaacggttg ctggagatga
     6541 atatgtttca tgagcaatct ctactataga taattctggt aatattgatt cattttcatt
     6601 tataaattta attatttcaa gttccgtctt ggttagattt tcagaattac ttttgtttag
     6661 ctcaattatc atatgttatc tccccttata taagttttat ttgtctaaat ttatcgagta
     6721 atataatata ttacctctta actacttatt tttatataca attaagattt agtcaatgtc
     6781 tatatatgta aatttataat ataataattt tttgcttaaa gtttattatg ttatcttata
     6841 gcattttaaa ataaaaaaga attgtacctc taccaaaatt tggatgaagt gcaatagata
     6901 ttccttatac tgatatgttt atgattaaaa gtataaatat aaatttcgct atgaatcata
     6961 taattaataa atcattttat ttacaattat aacccccctt ataaaactat attaataata
     7021 tatacagtat ttataagggg ggttgtttat atttaaaaac tatttacttt tcttttaatt
     7081 cttctccatt agttcttatt acattttgat accaataaaa actatctttt ttataacgtt
     7141 ttttacttcc atttccattg ttatctatat ctacatatat aaatccatat cttttttcca
     7201 tttcttgaga tgaacaactt actatatcta ttggtgacca catacaataa gctaatattt
     7261 cggttccatc agctatagct tcacctattt gttctatatg ttttcctaaa taatctattc
     7321 tgtaactatc atgaactttt ccatcttcaa atttatcatg cattccaaat ccattttcag
     7381 ctatcattat tggcttttgg tatctatcat aatattgaga taaagtattg tataatcctt
     7441 ttgggtcaat tgcccacccc caaggatttg cttttaaata tggattcttt atattgtcag
     7501 ttggattcat ggtgttttta cttgctgata ctgttgtaga gtaatagtat gatagagcta
     7561 gaaaatccat agtattttct tttaagattt tatcatcacc ttcctccata actatattta
     7621 tgttattttc tttgaaaaat ctcttaatat agactggata ctctccccta aaacttacat
     7681 cagtgaaaaa atattgtagc ctatttcttt ttaaagcaaa tactacattt tcagggtcac
     7741 aatcttctgg ataagatgta caatctgcca tcatcatacc tatcatcata tctgaattta
     7801 tttcttttcc aagcttctta gtcaaagctg tagctatgaa ttgatgatga atagcttgat
     7861 atctagcctc ttctacatta tctacttggt ctacacaaaa tgcaactgaa ttaaatgatt
     7921 cataatgtat taaatttatt tgatttataa ctatccaata tttaacttta tttttatatc
     7981 tatttagtat taattctgca tatctaacaa agaaatctac cacttttcta ttagaccatc
     8041 ctttatattt taatactaaa ttcaaaggag tctcatagtg aagtagtgtt acaataggtt
     8101 ccataccatt atctattact tcgtttataa gattatcata aaactttaat ccctcttcat
     8161 taggctctaa atcatctcca tttggaaata ttctagccca atttattgaa aatctaaaac
     8221 actttaaccc tagctctttc aaaagcttta aatcttcttt gtatgtatga taaaagtcta
     8281 ttccactacg cttaggataa tatccattat tatctttcat agcaaacttt attctttcta
     8341 tagtcatatc agactcataa ggtttagata tatctctatc tttatcatac atatgtacat
     8401 cagctaaaca ccaacctttg ccaccttcat tccatgcacc ttctaattga ttagctgcta
     8461 cagctcctcc ccataaaaaa cctttaggaa acttacccaa tttaatcact ccttatttta
     8521 tttatttaaa tcatttacta ttatttacct tatattctat agactttact atacctacta
     8581 aagacatagt tatatttaac tcgctgaata tggtcataag agtatcttga gcatgtgcaa
     8641 ataacagcga atgtgctctt ttctctccac cagcttcccc ttgtataatc ttggtttgag
     8701 ctctatgtgc ttctgtcata aacccttttg ctttagctag ttttaaatcg gcatttctat
     8761 aatcaaactt ttccaaatca ttaagagcat gtttaatttc tgttctagcc tcacctgcac
     8821 gaagtattat ttgcattgct acttctacta attcttcatc tattatatgt tcttcaattg
     8881 tcatatttat aatttccttt caatttctaa atttaaacct cttggctctc taattttatc
     8941 tcttctttct cataagcttt aaaaaatgga taccatatca taaatagcaa tactatattt
     9001 attgctaaaa caataagccc tctaacatct tgatttacca taaaagtaga tataccaaaa
     9061 ggaacatacc ataaattaaa caccttactt ggaattgtca caaatcccat actcattgat
     9121 aagtaagtag ttattgatat tactactgtt cctatccaca taggtaacat taaaaatggg
     9181 ttccatgcca ctggagctcc aaatattact ggttcattta tattcattat agaagggaca
     9241 atacatgctc ttcctattgc ttttattctt ccaactctgg ataaacacat cattataact
     9301 aatggtaatg tagcacccaa ccctcctaaa gttatccaac ctgtttgaat aacttcgtaa
     9361 acatatatat tagttgctac tcctcctgat gctactgctg ctgcattagc tgcaattgca
     9421 ccaagttgaa ttgaataagt tataggagat agtacccacc cacttattcc taaagaatat
     9481 ataaatacta ctaagaagtt taagagtaca aatccaggta atgtctgtgc tatatctgat
     9541 actggtttaa atactctaac tattatttca aacatgtcaa agttgagtcc ataaattaat
     9601 ccccagccta caaacattat cactgttata ggtaacatat tatcaaacca cttgcttaca
     9661 aaatctggaa gtgctgaatc ttctccgaaa aatgaaaaag aaccaaataa agacattatt
     9721 aatccagaga ataatcctgc aactagcgat acaagcattc cttctcctcc aaacctgttg
     9781 aagttaaata tagctccttc attcgtaatc tctggacgaa tcatcattaa cattaatcca
     9841 gcgcctgcaa gaccagctac tacactcata ctttcaaatt tcttattctt cattatttcc
     9901 ataggcaata aaaatgctat aaataatcct aaaagtccaa atgtatattg atttattgga
     9961 cttaaatcag gtaaaaagct tatgtaattc ttcaagacag aaactatagt tactaatgag
    10021 cctacaaata ccattggcaa aactttcatt atagttgatt gaactgattg tacccatata
    10081 ttcttagtca tattatttac aacaggagta aatttttcac ttaaaaaatc tataaattta
    10141 ttcataaaca ctctccccat aaatatttta tttgtttgat attagttcta gtgcttcttc
    10201 caaagccttt gcaccatcta gtcttccata tgcagtttta gatatacatg ctactggaac
    10261 gttatattca ttagcctttt gttgaatttc ttccttcata taatctaaat gtggtcctag
    10321 taataaacaa tctatttctg ataaatactt atctatctct gactcacttc tcgcttttat
    10381 atctgcttct ataccctttt tctttgcact ctttctcata ctagttgcca taaaacctgt
    10441 acttgctcct gaaccacacg ccaaaagcac tcttatattt ttcataatga tacctccaat
    10501 atttttatcc attaatttat ttcttaatga actttttatt ttattatgtt tatattgtac
    10561 ttaccaaata agttcatttc acataaatat atttcctaat aataggaaat cagttactct
    10621 taattaaaca tattaatata gtataatgta tcttataata cgtacattca attatataat
    10681 tatcatatca tccagatttt attttaaaac tatgcgaggg aatctaatga aaacatctaa
    10741 aaaagaacta tttttaaatt tcataaaaac acaacatgat tggatagatt catctacatt
    10801 ggctaattat cttaatgtct caacgcgttc aattagaaaa tatgtaaacg aaattaattc
    10861 caatggtgaa tttatattat cctctaaaaa aggatataaa gtaaatctca ataataattg
    10921 tcaaaccaag gttgatagtt cagaaaatat atcacctgat aacaggttaa atttaatttt
    10981 aaaagaactt atagttaata gtaatggaat taatatattt gatttaagtg aagaattgtt
    11041 tgttagccca gctactattg aaggggatat agtcaaagct aataaattta taggaagtta
    11101 taatttaaag ataaagcaat cgaaattttt attgaaacta attggaaatg aatctgcaaa
    11161 gcgcaagtta atgagctcta taatatttaa agaaacggga agtgattttt taagcttatt
    11221 tgatgtacaa aaaatatatc aagagtataa tttgactaaa ctaaaagaaa atataatata
    11281 tatattaaaa aaatacaatt tatttataaa tgagtatgct ataaacaaca tacttcttca
    11341 tttaatgata actatagaca gaataaaaaa gaataattac atagactctg ttgaagtagt
    11401 aaactacata gacaataacg tagatataaa tattgcaaaa gacatatcaa attttttaga
    11461 atctgaatac aatataactt taactagtgc ggaattatac tatttagtat ttcagttaac
    11521 aaacaaaact acagtcttaa actacaatca aatggatact aaatcattgt caaattatat
    11581 agatgaacac tttgttaaat taactaaaaa aataattaaa aatgtatatg acttatatct
    11641 tatagattta tctgatgagg aatttgttgt aaaatttact cttcatgtaa aaaatttaat
    11701 cagtagagcc aaaaataatc aagttttaag aaatcaaata cctcaaaagt taaaagattc
    11761 ctaccctttg atatatgata tatctgttta tatttgcaat cagattcaaa ctttagaaaa
    11821 tgtagatata gacgaagatg aaatatctta tatatctctt catgtaggtt cattctttga
    11881 tagacaaaaa ttattagaag ataaagttct atgtgcatta attactccaa attactatga
    11941 tttacaattc aaaatagtaa gagacctcga gaagagattt aatgaatcca tagaaattat
    12001 tcagattttt tcagatactc ataatcttga ttttgacaat aaggtagaca tggttattac
    12061 tactttacca attaacaata gatgccctat accttttgtt tatgtaaatc cttacctaaa
    12121 tagaaaggat tatgataata ttcaaagtaa atttattcag attaaagata gaaaaaatat
    12181 acttactgta caaaatcatt tagaaatgta ctttagtgaa tctttgttta tgaaaaatat
    12241 atatttagat agtgctaaag attatataaa atttatggga aatattctgt acgaaaataa
    12301 atatgtcaaa cccaattata tagatgatgt tcttataaga gaaaaaatgt cttcaactgc
    12361 atttaataac aatgtagcaa ttcctcatag catgaaaatg gatgctttaa aaactggagt
    12421 atgtttaatt gttaacgata aaccagttaa atggggtgag gaaaaagttc aaattatagc
    12481 tatgatacct ataaatgaaa aagaaaaaga aaaattcaat tacatatttg aaagctttat
    12541 tgaaatatta tctgagtgga ataatgttaa agaacttact aaggcagata actatagtag
    12601 ttttatgaat agaatagctt atcttattca aaatatctaa atcaattaat ttatttccaa
    12661 tatatgtttc ttaagttatt aacataaaat agataagctg tcacactaaa aagtctaaat
    12721 taatatgtga cagcttttta cctaatatac cttgttttaa tactacatat tttttgactg
    12781 ttgtatacta gttataagat tgaatatttg tgttgttgta gctgtcatat tctcactagc
    12841 agttgcttta tccattgctt cttctattgt catagctcta tttactatag aaaaataagc
    12901 atctataccc tcttcattac atttaactgc atcttctgtt gtggctccag ccaaacctat
    12961 tacttttacc ccatattttt ttgccagttt tgcaactcct attggagcct tgcccatagc
    13021 agtttgatta tccaatctac cttcaccagt tatgacaaaa tctgcatctt ttaactcttc
    13081 ctctaattta atttcttcta atattatttt tattccagat tctaattttg aatttaaaaa
    13141 tgctaaaaaa gcaaaaccta atcctcctgc tgctcctgct ccttctacat tagctacatc
    13201 tttacttaag taatttttaa ctactttgga aaaatttcta agcccattat ctaactcctc
    13261 aattatttct gatgttgcac ctttttgaga cccgtatatg tatgcagctc catttttccc
    13321 aaacagcgga ttatttacat cacaggctat tttaaactta cattcatcta attcctttaa
    13381 tctattttct atttttatat gtcttatttc atttaaaact tttccaccta atcctactaa
    13441 cttgttattt tcatcataaa actcaaaccc taaggcttga agcataccta tcccagcatc
    13501 attagtagca cttccaccta ttccaactat aaaatttcta tagcctttat ttattgcttc
    13561 ctttattatt tccccaacac cataagttgt agtgtataaa ggatttctaa gttcagtcgg
    13621 aaccaatgtt aatcctgatg cttgagctat ttctattatt gctgtacttg tattttttaa
    13681 taatccatat gttgcatcca cttttttagc tattggtcca gtaacttcta tagtctcttt
    13741 tttaccattc atcccttgaa ctaaagcatc tactgtccct tcaccaccat cagcaagtgg
    13801 caatatcttt acttgcgcat cttctttaac cttcaatatc ccctttttta ttgcatttcc
    13861 tgcctctatt gaagataagc ttcctttcaa agaatctata gaaattaata ttttcatatt
    13921 tatctactcc tttttattta ctctttattt taaatatata attttataag cttaatttat
    13981 cttttatttt atactctata tttcactttg tatacaactt ataccattaa tattctttta
    14041 tcttataaaa aagggagtgt ctcaaaatga actttagttc atgagacact ctttttgtat
    14101 aaaatcaaat atatgtttat tatttcaaca atgataagga ggattatctc tattttaaga
    14161 caattccttt ttagcactat ctaatcattt ttagcactat ttaaccagta tagttttaac
    14221 ttggttcatt aagcactcta catttacaga gttattatct attgatatag attctaattc
    14281 tttttcattt aggttaatcc atttagctgt tttaatattt ttgttaaata ctatgttagc
    14341 tgattttgtt tcttctttag aattataaaa tctaactact aatgcctctt gtttttcagc
    14401 cttttttaca acacttaaca caaggtcctt attagtttcc ttaagtaaac tatatgaata
    14461 atctacttct atttctgatt gattcaattt catagcatta tatggcattt tattataagt
    14521 ttgtattttt gttaaatact ctttagccat atttgcaaca tttgcttcta gtgtagattt
    14581 ttcatgagta actattgcta agtctatttt tatatctcct atcatttgag aatctggagt
    14641 aggcaacttt attcctgatg gacgacctgg gcgtcttgcc atttcttctt tacctaggaa
    14701 tcctacactt ctaaataatg ttatagctat tgtgtcaaat tttttcccta ctatttcaaa
    14761 ctctcttgta ctgtttgtaa gaacagctac tccatgctct ttgtcagaca atccaacgaa
    14821 tgttagcatt ggatatatag aatctggtct ttcactccat tcttcctgtt gccaaacttc
    14881 catagcatca tcatacacac ttctctttat atgtccaaac tgattatcag acacagaaaa
    14941 ctttgaagct atatttgttg gtatataagt tctaacccta caatctctta cttggttatt
    15001 tatgttgaat actacatcta ttatagcttt atcatttgga acatttataa ctatatcaaa
    15061 gtcaataaat gaatctactc tattattttt tctagcttgt atatcttttg gtacttttaa
    15121 tctataattt atctttatct cattattaaa tctatttttc tttatagtgt gatttgctaa
    15181 tatattatcg ttaaatatta atttctcatt tggaagtggt gaaaaatcat actcatctcc
    15241 ttcatctcct ccattttcca tcaataatac attttcaaaa gttttattta attttttatc
    15301 aaatatcttt aatgtcccgt tttcattaac ttgaatagta taaaagtcat tatctaccat
    15361 gtcacatact tcataagctt tttctaacat atgttcaact tcttttatca tatacgcctt
    15421 ataccccatt gcaggtattt tatctttaaa gtttattgta tataccataa atgaatcata
    15481 gtttccataa tgaacaattt gacggtctat taatcctgca tctatttctt ctttatgtac
    15541 cacttcaaaa tccaaattat tattatctct atcaaatatt tcaaatgctt tcattttagt
    15601 tataacttgg gcgcttacta tttcattcct atcgtaaggc attaagttaa atgctactaa
    15661 cttatctaaa gccatttcac aactcattgc atcaactatt cttcttttgt aaaatgttat
    15721 taattggtct actttttcct cagctaagaa aaatctattc attatttcct ggtgaacttt
    15781 atctgaacaa caacatccaa tgctgtcatg agcatgattt ttcattattt ctttccaaat
    15841 taactctatt aatccatgat gatattcaaa tcctagagaa tatgcaatag aagctagtgg
    15901 ctccaatatg ttggttatct ttgattctat tctagcattt gctgatttta tatccattct
    15961 tgttgagaat atacttctat gaaccctcat atatttccca tctaaaaatt ccccttttat
    16021 tgtatctata tcctcatttt tttctagctc tttaaatatg ttttcatatc tacttaagaa
    16081 aaattttctc tctggataac attcttttaa tttatatata acttcaaata tattcttttg
    16141 aattggcatt tggtcgtgtc catttggaag tattatatgg tcagtagttg cacccttatc
    16201 aagaaccggc aaatatttat ccattcttgt tcttagctca tcttcattca ttggtagata
    16261 ttttcctatt gcataaccta atggaagtat ttgaaccaaa acagaagacc catcatcact
    16321 cttccaatta aactctgttt tattagttcc ttttctttca gaacatcctc tccaaaacat
    16381 tgagtatttt atatcaaacc catttagtat ttgtggcatt tgagcacttt gaccaaatga
    16441 atctggcaaa tatcctattt tcatatattc tccaaattca tcacaatctt ttattccata
    16501 cagtaaattt ctaagtatgg attctccacc aactaccatt tcatcagttt gagtatacca
    16561 aggacctatt attagccttc cttcttgaac taattttttt attctctcct tacattcagg
    16621 cttaactgct aaataatctt ctaatatagc agtttgacca tccatcacat aatatggata
    16681 gttatgatta ttttctaaca tatccattat ttcttccata ttatttacaa gtaatattct
    16741 agattcctca gttgagaagt accattctct atcccaatgc atatgaggaa ctatgtgtac
    16801 attttttttc attgcttatc ctccaaaact tatctgataa tattttctgt ctaattaaaa
    16861 atccaataaa aatactactg agtttacaca attttttaat atttatttta tcactataat
    16921 taaacctttg atttaaacat ctaataacgt atcttttaat gtatctttct ttgatttttt
    16981 tgtttcctag ttattattaa taatattgct gatattatag ctccaagtac tgccgcaaag
    17041 aaccatattg atgctataat aaatcctgac atacttccct ctaaaagcgc caatgagaat
    17101 atccccgctc caggcacttg aagacctata ttgaagaatg ctactataga cccagttatg
    17161 gctgaaccag ctactaatga tggtataaca cggattgggt cctctatcat aaatggtatt
    17221 gcaccttcag ttattccagc caatcctaat atccaagttt gctttcctat ttccttttct
    17281 gattctgaaa ataaatctct gcctaaaaca gtagctattg taactccaaa agctgaaacc
    17341 atcttaactg atgcaaatat tgcataaggt ataaaatttc cacttgctat tgctcctatt
    17401 gcaaatgtat aggctgcttt atttattggt ccacctaagt caaatgaaac catagctcct
    17461 attattgcac ctagtagtat tgcatttatt ccagtcatcc catttaacca atttataaga
    17521 ccattgttaa gtgctgctac aggctttcct attacaaata acatcaataa acctactacc
    17581 aaagaaccaa ctactggata tacccaaaaa cttacgaatc cagctaatgt gcctttggct
    17641 ggtatattct tctttaaaaa ttttactata taaccagcaa gtaaacctcc taacatacct
    17701 ccaagaaatc cactacctat taaatcagct gctagacctg ctgcaaatcc tggtccaaga
    17761 cctggttttt cagctagtga gtaagacata tatgctgata atactggaac catcaatgta
    17821 cctaacatcc ctccagaaag ctttcttaat tgccataacc aagaaccttc ttggttgtat
    17881 agttcttgta gaccaaatgc ttgagatgct aatactgcaa atgcaagtat catacctcct
    17941 gccacaataa gtggtattat atgagatact cctgtaagta ttgctgattt tacatctttt
    18001 ttccatgatg atttttcaca atatgatata tcagatgatt tttctatata atcaccttta
    18061 ccttgttttt gtgctacttc taaagcttcg tatattattt cttttgctct ttttagtgga
    18121 gctgctactg acgtttttat gtttggcaaa tgttcaaatc tttctgtatt tttaggtgct
    18181 acatctgttg catatattat tgcatctgcc ctttttatta tatctggtgt atgagggtcc
    18241 accataccat tagctccttg cttttctaca tacacatcca cacctatttc tttaccagct
    18301 ttaattaatg cttctgctga catatatgta tgtgctatac cggcagggca tgctgttatt
    18361 gctaatataa ttttttttga ttcattttga atcctaatct cttcttttat ttgttctttc
    18421 ttttctttta tatctaagtt attaaataac tctttcttag tttttgaatt taacaacata
    18481 ttcatataat cttcatttga cagtctagta actaactcag aaagtatttg tatgtgtgta
    18541 tttccacctt cattttctgg tactgctatt aaaaatatta tctctacttt attatttggg
    18601 tctacgcttt cccaattttc tattggtgtc tctaaggtca tagctgcaaa tgcagcttct
    18661 ttaactgact tagattttcc atgaggaatt gcaagccctc tttcaaaacc tgttggagat
    18721 aaagtttctc tgtacattac agcttctaaa tattcctcat aagatgttaa cttgccttca
    18781 tcatatagct ttcttgctaa gaactttatt gcgtcttcct ttgtttttat tttttgtttt
    18841 aaaaaaacaa ggttttcatt tgtgattttc ttaaattcca aaattacgcc ctccttaaat
    18901 catttaatgc tatgaatata gcataaaata aatcatgaaa atgttttttt gaaattatac
    18961 acacaatttc ttcttaaaaa ataaagattt gtgtatttat acacaaatct ctatcttaac
    19021 cagtattgct ctattattgc tctcataatc atcataattg gaaccttatc tgatatattg
    19081 tacccattta cttgtttaat tgttgaatag caatataaac ttatatctgc aatttgttgc
    19141 aattgatttt cacatatatg tgttagagct actaccttac aacccttatt aactgctgtc
    19201 tttgctgctt ctataacttc tttcgtttct ccacttaaac taagtgcaaa aaacatatct
    19261 tgttcaccta attttcctat atcatgaagt acttcatgtc tttgactata gtaagttata
    19321 ttatcatgcc cacctctaat ttcttttact aatatctcac ataaaggaat gttttgacct
    19381 attccataaa ctataatact cctagatttt tgaattatat ctgctacttt atttattttt
    19441 tcataatcta ttagctctaa tgtctttttt atatctgatt caaaaccatt ttttggctct
    19501 ttattctcat tttttaactc ttcttttaaa ttatttttta gttgagagaa accatcatac
    19561 tccatttttt tacatagcct aactatggta tttgggacag taaaaaattt atctgctaaa
    19621 gattgtattg ataattttac tatttcttct ttgttttttc tcatatattc aattatcatg
    19681 tcttctgtat ctgtaaattt atactcatat ttttttgctc tttcataaaa ttccatagtt
    19741 tctcctccat ctattttatt attcataagt aaaattcaaa aatattagct aaaattttcg
    19801 ttaatataat tataatttaa tatttttaat ttattaatgc atactactct ttttattata
    19861 cataaaaaat gtgtacttcc tatattgata gagatgattc aagtttttat tttgatacat
    19921 ctctatcaat atttttttag aaaatacaca tttttttcaa atacttattt ttaattaaaa
    19981 ctttaggtaa tctattcttt atatataatt attagacttg aaaatatctt taaaatcaac
    20041 caattcaata ttatttcttt ttaaccacat tttaacttcc ttatcacata gcattgcatg
    20101 ttcatatact ctctcttgaa taagagaaga tgattttata agactataat ctataaatcc
    20161 tggatgagta accatcaatg ttatatcttt atctaaattt atatattttt cttcaaataa
    20221 tttaacaaaa gatttattta acttgtaaaa atcattctga ggatatttac ttggcatgct
    20281 aatacatgaa tccaaataat agggacaact tggtataccg tattcttttg cagcatcaca
    20341 cattgctgat attgatgcat cttcttctat accatgtata tcaatatact caggcaattt
    20401 actcatatac aacttgtaag tttcaatttg agcacaaact tcttcatata cttcttcata
    20461 attaaaaacc tctttacctg tcagtagttg cgttcttctt attttgctag aaacaaattt
    20521 tccaccttca tctaacaaat tctttatctt aatatgttct ttgcaacaag gttctcctat
    20581 tactaaatta acatgtaaac caaaacatac atcatcattt tttaccattt ctatagcttg
    20641 tcttgaaaaa ggcatattca ccatgattcc agcacttgta acaataccgt tcttataaga
    20701 agatattatc cctagtgtaa caccttctga atatcctata tcatctgccc taactactaa
    20761 tttcttcatt ttatgcatct gcctgctttc tcaactcatc aagttgcatt tccatctcat
    20821 cctttacttt aacaacatcc gttcctatga tgatttgaac tgatgtatta ctcaatttaa
    20881 ttaccccttt ggctcctgtt tttttaatat tatcatcatt caaaatagtg gcatccttca
    20941 cttctaaacg taatctcgtc atacaattat taagagtcac tatattttca taaccaccac
    21001 aatttttaat tatctgttta gccatatatg aaaaattacc atgagaaagt tttaaattct
    21061 tttctttttc actaacttta tcacctatct ctatctcaac ttcacgtcct ggtgtttgca
    21121 cattatcttt tattattata tatttaaatg ttacataata taatacaaag aatacaactc
    21181 ctacaggtat aactaaccac gctttatctc cataactaaa acttaagaca tagtcaatta
    21241 tacttgctcc attaaatgaa cccaagcgag cacccagtaa atatagtaca actccaccta
    21301 gaccagaata aaaagaatga attatatata actttggaga tgcaaataaa aacgaaaatt
    21361 ctattggttc agttactgtt gccataaaag aagtcaatgc tccagcggtc attgcacctt
    21421 ttacttcttg cttcttttta tcttgtgctg ttttatatat agcataagaa atagcaggta
    21481 ttgaaaacat cataattatg taaaatcctg tccagaagaa tcctgcattt gggtctcctg
    21541 ccataaatct aggaatttct cctcttataa cttctcctgt tgatgttgta tattcaccca
    21601 tttcaaataa tatgtaagca tttaaaactt gatgtaaccc agttggtatt agtgcacggt
    21661 tacagaataa aaacaagaat attcctattg caccagctcc tcccaaccaa aaaccaaatt
    21721 gattcattgc agcttgtgga tatatccata ataaactaaa tatatatgat aaaattaaag
    21781 caaccactgg tgatagtgta attacacatt tttctcctgc aaagaagctt aaaaatgctg
    21841 gaactttaac tttatatgtt ttattgtaag tccatgctat acaagctcca acaattatcc
    21901 caccaaatac acctgcattt aatgtatcat tcaataactc taaatttgac ttaaatatca
    21961 tatatgcaac tacggctgca accattacac ttgtcttgtc tttagacttt gagtaggcac
    22021 tagctgctcc taataaaaat aagtagtcca catttgaaaa tactatatta gatatattag
    22081 atatagctgg cacattgagc atatttggac caaataataa tcctaatata cctgctgctg
    22141 gcattacagc tattggcact agcatagtct ttcctaaaag tgacagttgt tgcatcattt
    22201 tgtttaagaa cctcttcata tccaatcccc tttctatttc ggtttaattt tttgtgaaaa
    22261 cgatttattc acattttata ttttaaatat tatatttgtt taaacaaatt gtcaatattt
    22321 tttattcatt ttatttttat catttagttt aacatataaa tccttttaag tgtattgcta
    22381 tatctagtct atttttttaa ttcatttttt ttatttcaaa aatatgttta accatgcatt
    22441 tgaaacaacc cacaaaataa ttctattttg tcctttttat ggttgatatg attctatttt
    22501 cactatacat gtctaaacac atttgatata atttgttcat atcagttcaa taatattact
    22561 aaagaccttt aaggagggat aggggtatat ggctaaaaat aaattttcag agatatatga
    22621 agtattgaaa gaggaaatac ttgatggtaa atatacaagt aatatgatgc tgcctactga
    22681 acttcaactt attgaaaggt tttcatgtag cagaaacact gtacgtagag caatttctca
    22741 attaaacaca gaaggatatg ttcaaagtat aaaaggcaag ggtgttgtag tgttagaaaa
    22801 tagttgttct aatgattttt ttctaaatat gcacaatttc aaaggtgtag aatcgattgt
    22861 agaagataaa aaggttaata ctgctactag tgttctccac ttcagcaaaa tcttaattga
    22921 taataagtta tctaaaaaga caggttttaa agttggtagt gaagtatatt accttcacag
    22981 actaagatat attgataaca tacctaaaat tctcgacatt aactattttc tatgtagtat
    23041 agttaaagac cttgatgttt ctatagcaca aggctctatt tataaatata tagaagagtg
    23101 tctaggtaca aaaattgttt catccagaaa gatatttaaa attgaaaaag ctacagagct
    23161 tgagttaaaa acgctacctc taaatgatta taattgtgtt ggtgttatta aaaatagtgt
    23221 atatactgat gatggaaagc tttttgaata tactgaatca aagcatacac ctgaaacatt
    23281 tgtattcatg gatgttactc aaagatatta aaaaaattat gtttaattaa acataatttt
    23341 tttacgttta gacactttat cccatactaa cctcactatt ctatatacaa ttatatgttt
    23401 atacaaataa ttatttattg tatattttag ctttaatatt agtttaacgc ttgacaattt
    23461 gtttaaacat attataatta acttataaat tacaggaggt atcaagtatg aaaaattggt
    23521 ggaaaaaagc aactgtctat caaatatatc caaaaagttt caaagatagc aacaatgatg
    23581 gtataggtga tataaatggt attatagaaa aattagatta tctgtattct ttaggtgttg
    23641 atttattatg gctgactcct atgtacgttt ctcctcaaag agataatgga tatgatatag
    23701 aagattatta taatatagac ccaaaatatg ggacaatgag tgactttgaa aaactcttaa
    23761 aagaagctca taaaagagac ataaaaataa tgatggatat ggtcctaaac catacatcta
    23821 ctgaacataa atggtttaaa gaatctaaaa aaagcaaaga caatccatat cgtgattatt
    23881 atttctggaa agatgctaaa cctgatggtt ctgttcctaa taactggata tccagattta
    23941 gtggaactgc ttggaaatat gatgaaacca ctaaccaata ttatttacat ttatttgaag
    24001 aaactcaagc tgatttaaat tgggaaaatg aaaaggttcg tgaagaatgt tacaaaatac
    24061 tagaattttg ggctgataaa ggtattgatg gttttcgttt agatgtagtc aacctactct
    24121 ccaaaacacc tggattacct gatgacccta taacagggcc aaaaggagat ggaagaactc
    24181 actacgctga tggtcctcgt atacacgaat atttacataa tatgaatcaa aaagttttca
    24241 aacctaaaaa tatagttact gtaggtgaaa tgtcatctac tacacctgaa gagtgtatca
    24301 actatactaa agaaaatcga gaggaactat ctatggtttt tagcttccat catatgaaaa
    24361 cagattataa aggaggcgca aaatggacta atgaaatgtt ctctctggat aaattaaaaa
    24421 aagcacaatc tgactttcaa tataaaatgt atgaaggaaa gggatggaat gctctatttt
    24481 actctaatca cgaccaacct agagctttat ctcgatttgg agatgataga ttttttcgtc
    24541 aagaaagtgc taagatgtta gccataacat tatttggtct acagggtact acatacatat
    24601 atcaaggtga agaaataggt atgacaaatg cctactttac taaaatagat gaatatgatg
    24661 atgctgaaag taagaatgca tactatcata tgattaaatc tggtattgat gaaaaggagg
    24721 cattgcttat acttcaacaa aaatccagag ataatgcccg tactcctatg caatgggata
    24781 atactattta taaggggttt tctaatcata aaccgtggtt aaaagttaat aatgacaata
    24841 ttagtgtaga aaatgagctt aatgattctc gttcagtatt ttatacttat caaagattaa
    24901 tcaaatatcg taaacaatat gatattttta cagaaggtac ttatagattg ttagatgaaa
    24961 atcataaaaa tctatttgtt tatgaaagag agtataaaaa ccaaaatctt cttgttataa
    25021 gtaattttac tcatgataat gtagtatata aattacctga aacttatttg aataaattaa
    25081 actacacaat acttgaaacc aattacacta gagctataat tgaaaataaa atggaaataa
    25141 aaccttacga aagtataatg atatattata aataaagtta tttatgtaaa aatcggtata
    25201 acaccctggt gttataccaa ttttactaaa ttcaaagtaa tatttataga atatctatag
    25261 attacaataa ttatttgact tctacatcat tatatttctt tgaatatact ttagtcatat
    25321 agaaatatat aggaactaca attatcatcc cacctaatcc ccaaactatc ttcataggtt
    25381 catcaatacc tgcttttatc agtaataata tacttataat aactgcaaat attggtataa
    25441 ttgctcctcc aggtattcta aaatttacat ctttaccagc atacttcttt ctcaatatta
    25501 gtacagctaa acatgtagga atatattgaa taaatctaga tacaacactt atttgagata
    25561 atgttgtgaa tgaacccgac catgctatta gtaaagttcc tatgacagat actataattg
    25621 ctatataagg agctccattt ttattagttt gtcttataga cttaggcatt aatccttgtt
    25681 caaccaaagc agcagcagac cttggtgtaa atatggatga tgcaatattt atacctccaa
    25741 cagatactat agttcctatt gtaattatat acccacctat aggacctaac atcttaatgg
    25801 cagcatctgc aacaggagta gctgaccctg ccaatccatt acccaacacc cctatactta
    25861 ttcccagtat aagcatatat attactgata ctacaaacat taccattacc aatgctttag
    25921 gtacattttt cttagggttc tccatatctt ttgctgcaac agctaaagat tcaaatcctg
    25981 taaaagcata aaataaagtt atagatactg ctacaaaagc tgatgacgca ctttgacctt
    26041 tttgtattat tacaaatggg gtaaaattag aaccctttat aaagaataat cctacagcta
    26101 taaataaaac tattggtaat aattttccta tagtcatgat attatttaag tgcttagatt
    26161 gtttcatccc cataaaattt ataatcccaa gtcctacaac taatatacca attattatat
    26221 ttttaataag tggttctgct gcttgtggcc atacagatga tagtgttaca gcaaagaaat
    26281 ttgccatagt agcccaagca atcatactaa ctatccactt cataaatcca acttcaaatc
    26341 caacaaaatc tccaaatgcc tcttttgcat aaagataggg ccctccatca gtatcaaacc
    26401 aactacctgc ttcagcaaaa cataaagcaa tagaaacagc taaaagcata caaaaaacta
    26461 atactaatat acttgctgga cctatatctg tataagcatc accaggtaac ccgaatatac
    26521 ctgaccctat tattgcattg attcctaata gtacaatact ccaaaaacct aacttaatgc
    26581 ctttttccat tgttattccc cccaaagcca acttaatata tttatatttt ttatttagat
    26641 gcttctttaa ctattgccat aaatattttt ttcatattat cataatccct agtcatcatt
    26701 tctggatgcc attgtatccc tattacaaac tcacttcctt ccttttctat agcttcaatt
    26761 aaaccgtctt tagatgtagc tgaaacaata tatcccggtg ctactttgtt gacaactaag
    26821 tgatgaaaac tattaactaa aacttctttt tctcctagta tttcatataa ttttgtcccc
    26881 tcctttatct gtactgtatg tgtaggtaca ttagatagat gttgctgatt gtgtttaata
    26941 taagcacctt caattaaaga taaatcttga tacaaactac ctccttcagt tacatttata
    27001 atttgttctc ctctacatat acctaaaact ggttttttca tatctaaagc atgttttaat
    27061 aatttcatat caaaactatc tctctttggt gatattgccc ccaacttatt atgaggttct
    27121 tctttccata tgagtggatt tacatcttgt cccccagata gtataagcgc atctatatta
    27181 gatacctgct cctttataat ctcatcatca tatacaatcg gtacaatata tggtatagct
    27241 ttacacataa ctactgattg tatataatca ttatttacat aagccctttc atagccagga
    27301 aacattcctc cttcatcaat tagtaaatta cctgatattc ctattattgg atatttcttt
    27361 ttagccaatt tcttcacccc ttaacttatt tgtgaacaat aaaaaatgct acttctctta
    27421 aagagcaagt agcataccaa gattttattt atttgaacat tataatttat tttagaaata
    27481 ctttttttga atacgagtaa tataaataac tttatttact tatattttat tttaatcctg
    27541 tttagtttga cttttttaaa ttattttaag tacaactaat aggtaaaata ggcatttaga
    27601 ttttcctttt tatcaaccta ttaaatactg tttaatatat tttttcccct tctatttaat
    27661 tcacttccct ttctaccaga ttcacatatt atcaaatcca gctcttttaa catattaagt
    27721 attcccctta cctcaccttc tgttatattg tagcctaatt catttattga attaacaata
    27781 ccatttctac ctatattttt attaagatta ttggcatttt ttaaacactc cattactaac
    27841 tttactttct ctatagatgt tctatccttt aatatactta tttctttact aaaatcttca
    27901 gttatattta ataagttatg aggtaaatca tttattaaaa taacatcctc agaactcata
    27961 atgttaatat acatagcagt attttttaat tctcttatat tacctggcca ttcatatgca
    28021 ttaataatgt cttgaacatc ttgactaatt gctctcttcc ttttcataaa atgctttaac
    28081 ataacttcta tatcttcttt tcgctctctt aaaggtggta tatttatagg caatacattt
    28141 atcctatagt ataaatctgc tctaaatcta tctcctttta tcatttctaa taaattttta
    28201 tttgtagccg caattactct aacatctata tctattacat tttctcctcc aaccctcatt
    28261 acttgattct cttgtaatac tctaagtaat ttgctttgta agtatatagg catgtctcct
    28321 atttcatcca aaaatatagt tccattgtta gcctgttcaa ataatccttt tttacctcct
    28381 tttaaagctc ctgtaaaagc accttcttca tatccaaaaa gttcactttc aagtaaattt
    28441 tctggcatag ctgcacagtt tattgctata aatggctgac tatgtcttgg tgatgcatta
    28501 tgaatagact gagccatgag ttcttttccc gttccacttt ctcccgttat aagaattgta
    28561 taatcagatt cagctatttt catacctaat ttcttagttc tttccatctt cgttgaaact
    28621 gtttttatat catcaaaagt atatctagca acttgtccct tttctctaag cttctttgat
    28681 aggttttgct ccaattttct tatgtaagtt atttcttgaa tacaaaaata atatccagta
    28741 acccttccaa aactctctat attatactta tttacgttta agtacttatt attaaatttt
    28801 ataacttcat catgtgcctt actcaaacta aaaatctttt taatattctc attctctaat
    28861 tcatctatat ttttccctat aatattactt tgaatatcta gtatatccct taaactatta
    28921 ttacaaataa ttatgtttcc cttatctgat accattgcca tacctaagtt tgataaattt
    28981 attatggaat ttagttcatc tgcttttata gtaagttctt tgtatttagt atttattcct
    29041 gaatataaac taattatttc atctgtatac ttaattagcc tctcagtaat ttctttatca
    29101 tccagtttga gttttgctat tatttgaata aacgttgaac tatcaatata cctatggcct
    29161 aaatcaataa cttcttctat atattttgga actaaacttg actctcctgg cgttatagct
    29221 atcttaatat ttctatattc ttcattggga ttgtatggta taaaattcag atgatttatt
    29281 cctatattgt ataaattaga tattgtttcc aatacagtat gcttattatc gtttactact
    29341 aatacatcta ttccttctgg caatgaaaat aacttatata tatccttttg ctttatgctc
    29401 ctatttatag ttattatctt tgagacatct tctacatatt tttttatttt taatactcta
    29461 tcatctatca ttaccaatat tatgtcatct tttattaatt ccttctctgt tattttattt
    29521 aaataataat tttttatcat tactttattt ttaaatatca tttctagatt tttatttaag
    29581 aaacattcta atttagacaa cccatcagtt actactgcta ttgttttcat ttgattactc
    29641 cttttaaaat atactctact cttttataat atacatctga ttttcacttt aacagcacaa
    29701 aaaattgtac atttagtata ctaacatatt cctttattag ttttatgtcc ctaaatatca
    29761 tagttgcaat atctatcata tttcttaaac ttctataaag caaaaattgt gccaaattaa
    29821 taattttctt aattttattc taaagttcct caatagtatt tacagactta gcacttatag
    29881 cttataagtc tttatattat taatctattt ttataggtta attgtacaaa tcatatatca
    29941 cttttaccca ttaaatgcat tataaaaaag atggaataac gattcgttac tccacctttt
    30001 ttgtaataaa aaattgtatt tataaacact atttactact cttctaagta caaacagtat
    30061 ctacatacta taacctttca ccattagatt ctattacttg tttataccag tcaaaactct
    30121 ttttcttaat cctagacaag tctccacttc catcatcata cttatccacg tatataaacc
    30181 catatctctt ggccatctca ccagtagatg cacttactaa atctatacat ccccatggag
    30241 tatatccaaa taaatcaaca ccatcttcta cagcttcctt catttgctct atatgtgctt
    30301 ttaaatactc tatacgatag tcatcattta tcactccatt agattctttt ttatcataag
    30361 ctcctaaacc attttcaact accatcatag gtattctata tctatcataa atttcattta
    30421 gtgcatatct aagcccttca gggtctattt gccatcccca ttcactagac ttaagatatg
    30481 gatttttcac tccacctaat atgttgccac ttgtattatc ttctttagaa tcactactta
    30541 cacatgtaga catataataa ctaaatgtat aaaaatctac acaaccttct tttaaaattt
    30601 ctaaatctcc tttttccatt tttatttcaa tattattttc tccaaaatat ctattcatat
    30661 aagttggata ttcgccacgc acttgtacat ctccacagaa ataattcatt actctactat
    30721 ctctttgagt tttaactgca tcatctggat tacaagtata aggatacatt gttgcaaata
    30781 tttgcataca tccaacttga taatttgaat ctatttcatg agctaactta acagccttag
    30841 cacttgctac aaattgatga tgtaaccctt gaaaacgaag ttgaggtata tctacttggt
    30901 ttataaaatc tgtagtccct tcatttaata ttccttgtga taagtagcct cccataggca
    30961 ttgtagctga atttatttca ttaaatgtca gccaatattt tacttttcct ttatatcttt
    31021 taaatattgt ttcacagtaa ttcatataca agtctattac atctcttgaa acccatccat
    31081 tgtatttctc tgttattgca aatggtattt catagtgaga tattgttatt aatggttcta
    31141 tattatattt taaacattca tctatcacat tttcataaaa tttaagtcct gcttcatttg
    31201 gttctttctc cattccagtt ggaaatattc tagtccatgc tattgaaaat ctaaatacct
    31261 taaatcccat ttctgcaaat aaagctatat cttctttgta tctgtgataa aaatctattg
    31321 cttcatgact tggataaaat gttccttttt ctataactct cgttattctt ttacttactt
    31381 tttgagaacc tccagtacac atatctgaac aagaaggacc tttcccatct aaattccatg
    31441 ctccttcaca ttgatttgct gctgttgctc caccccataa aaaatctttt ttaaatgaca
    31501 tacctatatc ctcctattat aattcttcac catttgattc tattactttt ttataccagg
    31561 caaaactctt ctttttactg cgttttaaag ttccatttcc atcattatct ttatccacat
    31621 atataaatcc atagcgcttc ttcatttctc cagtacttgc acttactaag tcaatacatc
    31681 cccatggtgt gtacccccat aaatcaacac catctaactc aacagcatct atcatggctt
    31741 ttatatgagc acgtaagtaa tcaattctat aatcatcatt tatatttcca ttttcatctg
    31801 gattatctac tgctcctagt ccattttcaa ctataaataa tggtttttga taacggtcat
    31861 ataaagagtt catagttatt ctaagcccta gtgggtctat ttgccatccc cactcacttg
    31921 catctagata tggattttta agacttgcaa aaacatttcc ttctgttaaa atatttactt
    31981 ttgggtctgc acttgtaagt cttgatgagt aatatgagaa tgaaataaaa tctacggtgt
    32041 tctctttaag tatcttttta tcatcttctt ccatcactat atttattcct tttctctcca
    32101 attcttttaa tgcatatgtt ggatattctc cacgagattg tacatctata aaaaagtaat
    32161 tttccctatc tttttctata gatttccata tatctagtgg gttacaagta ttaggataag
    32221 tatttccagc tgctaacata caccctattt taaattcttg atttatgtta tgtgctagtt
    32281 tagtaactaa cgcactcgca actaattgat gatgtgcagc ttggtatttt atttgttctt
    32341 cattttctcc ctcttcaata tagagacctg cacctataaa tggtaaatga agtatcatgt
    32401 ttatttcatt aaatgttagc caatacttta ccttaccttt aaaccttgta aaaataactt
    32461 cacagtattt tacatataag tctactagct tacgatttct ccatgctcca tattttttta
    32521 ccaaatacat gggaacatca aaatgacaga ttgttactac tggttctatt ccatacttta
    32581 aacactcatc aaataaatct tcatagaatt ttagtccctc ttcgtttgga acatcctcaa
    32641 ctcctgttgg aaatatccta gtccatgcta ttgacatacg atagcattta aatcccatct
    32701 cagcaaataa ttttatatct tctttataat tgtgatacat atctattgct tcatgagctg
    32761 ggtaatagtg ttcatcatca cattcaaaca ttttcatttt cccagccaaa actggaaatc
    32821 tatcttttcc ttgcggtgta acgtcaacat tagctagacc tctaccatct tctttatatc
    32881 caccttcaca ttgatttgct gctgttgctc caccccataa aaaatctttt ctaagtgcca
    32941 taattctacc tccaataatt tatttatagt aactttattt taaaataaaa atacatatat
    33001 ataattctta attaatagaa ctctctttta cgctaaaaca gttatcaatt tctgactatt
    33061 atttacatgc ttttgtgtat tttctacaat atccacataa tcagaataat tagtaacaac
    33121 tactggtgtt tctaagcaat atccttcttt tgaaatactt tctatgtcaa aagttattaa
    33181 tgtctgacct tttttaatct tatccccttg attaacaaaa gatttgaaat atttaccttc
    33241 caactgaata gtatttaacc ctatatgtat caatagttca caaccattat cagatataat
    33301 tcctattgca tgttttgttg gaaataaagt cattacagtc ccatcaaatg gtgccactac
    33361 ttttccttct ttaggtgtta ttgctacacc ttttcccaat atacctgttg aaaacgctgc
    33421 atcttgtatc tgctccaaag cctttacttc accttttatt gggctatata ctacttctct
    33481 tttgtttttt tctataacgc cttgcccatt atcatcttta gaaatttcta ctatagaatc
    33541 atctttccaa aaaaataatg ttaaaacaaa tcctactaca atagctacta ttattgctat
    33601 tatagttgca taaacacctt tcatatctcc agttgttgga tttatataat taggtatacc
    33661 aaatattcca agtccaccca ttacataact ttttacattc attatcccca ttacagctcc
    33721 tgccacagca ccacctacac aactatatat gaatggtgtt tttcttggta aagttattcc
    33781 atatattgca ggctctgtta caccacatat ccctgaaata actgcaggta tacacagctc
    33841 ttttatcttc ttatctttta atttaaagta cattgctgct actactgctg tttgtgcaaa
    33901 agatggtaca aatgctccca ctaatattgt gtcaaacccc atagtcataa ggtttgacat
    33961 agctattggt actaatcccc aatgtagacc aaatattaca agcacttgcc acaataaacc
    34021 agttaatgca ccacataaaa ttggtgaaaa gttaaataaa gctaaaaatc catttccaac
    34081 tatattagaa gcataggtag ctataggccc tataaccata aatcccaatg gtaaagaaac
    34141 taataaaact gccattggta caaagaaatt ctttactaca tctggtatta ttttagcaaa
    34201 cactttctcc aacttagctg ctaataatac tattaaaatt actggtacta ctgttgaagt
    34261 gtagttcatt gctattactg gtaatcctaa gaaagttata tatactggtg attcaaatat
    34321 tgttccagta aatagattgt ataaaggttg acctgtagat gctagagtat ctaattgaat
    34381 acctgaatag cataaaactg cacctataag cattccaata aatggtttta atttgaattt
    34441 cttagcagct gtatatccaa gaattattgg catatactgg aacattgcat ctccaattgc
    34501 atttaacata atatatgcac catcagtttc tccatacaaa cccaatgctg taaatactgc
    34561 attaaatcct tttatcatac cagcagcaca cagaactcct aatattggtt gaaaaactcc
    34621 tgatattatg tctataaact tattaagtaa tccttttggc gcatctgagt ctgaattatc
    34681 tattgtatct cctcctaatc cagccaaatc gcacacaact tcataaacat ctggaacatg
    34741 attaccaata actacttggt attgacctgc acttttcatt acagtaacta taccatccat
    34801 tttttttagc acatcatcat ttgctattga ctcatctttt aatttaaaac gtagcctcgt
    34861 tatacagtga gataaactgc taatattatc ctttcctcct acattcgcaa tgatttcttt
    34921 tgatagttgt tcatactttc tcattttttc tcctccattt tctttttgaa atatgaaggt
    34981 ttggccaact taatgtcacc acccaatata tataaaccta attattagga tttatatctg
    35041 acttaatact tttacttacc actctttcaa cgtgaatagt taagtataat ttttcctcat
    35101 ctgttaaatc gtagttgtat tttttatgta taaattcatc caacttttct atgaatttat
    35161 aagcattttt atatttaact tttattacat ttaataaatc atcttcatta ttttcatgta
    35221 aattattact aacaattcgt tctgcaaaat actttaagtg agtaataaat ctatgataat
    35281 aaactgaatc ttcatcaaat actattctgt aatggtattt aaccatattt gaaatttctt
    35341 gtattatttg tgtaatctca tatatagttt ttatactttg atccatttgt gcattaacta
    35401 tatgcagtgc tataaatcca gcttcatctt ctgatagtct aatttcaaat tttttttcta
    35461 tataatccaa tgcctctaac cctattttaa attcactctt ataaaatctt tttatatccc
    35521 aaagtaatac attttttaca cttactcctt cacgagctct ttctattgca gtatacatat
    35581 ggtctgttaa agaaatataa atactatcat ttaatctttt ccctagtttt tctttagcat
    35641 ataaaattat ttcatctgat aattccatat atcttatagg aatatctgct attagttctt
    35701 taaatttatc agatatattt gggtctgaaa gtttgaatat cttatcaatt ttatcctttg
    35761 atatatgctc tcctgtacgt tttttataag ctaaacctct ccccataact actatttcct
    35821 tatgattttc atcagttgta ataattacat tattattgag tattttttca ataatcattg
    35881 tgatgccccc tttattagca ttaagttaaa ataaatcttt tttaattatt tagaatattt
    35941 aaaatgtaat ttattacata aaaaaaaacc tatttattac acattaaata gactcccatg
    36001 agtcctgagt aataaatagg tttagcttgt atttaaacaa tcactatccg tttgtatttt
    36061 attttaatta ttatttatta tatgttagca aatttatttt ttagtgtcaa ctttttctca
    36121 atattttaat tttaaataaa ttatataaat tgaatcttca aagtactatt tttatcaata
    36181 aacaaaataa aaaaggtatt actttcttta agtaataccc atttattttt aatttaagtg
    36241 gtttttctta atctcatcaa gtagtccatc ttctaaataa atttctaatg cacatcctac
    36301 cattgccttt actgctttgt gaagtttatc ataagcttcc tcagaatttg cataatttag
    36361 aaattctttt tcatgaacat ataccattgg acttatatct aaggctattt ctgtatacat
    36421 agtagggcaa acttgactta cattacctat atctgtagaa cctactggac catctttacc
    36481 ttctaatata tctgttatac ctgcctcaat caaattattc tttgttatct tttgaagtac
    36541 tttgttattt attatgttgt caaaagaatt ttcaaagtat ctataagaaa gcttagctcc
    36601 tgtcattaac tctgcacctt ttgcacagtt tataactttt ttagttactt catctaaata
    36661 acttctttca gcagctctta cgaaaaactt acaagctgct ttttctggaa ctatatttgg
    36721 agcctctcct ccatttgaaa ctataccatg tattctaaca tctgatttta catgttgtct
    36781 tagtgcattt atcccagcaa aagtaagttg tactgcatct agtgcattga ctcccatatg
    36841 agggtgacta gctgcatgtg ctgatacccc ttcaaaacta aactctaatg aatctatcgc
    36901 caaagcctta cagtttatat tattaaaagc ttctatatgc atctgataac ttacatcaac
    36961 atcatcaaaa gctcctgcct taactaaatc acattttcct cctgttgttt cttcagctgg
    37021 tgttcctatt agtacaattt ttcctttaaa attttctttt aacttactta atactataca
    37081 agctcctagt gtagatgctg caatccagtt atgtccacaa gcatgagctg gttctttatt
    37141 ttctccatat ccaggaagtg catcatactc agctaaaaat gctatggtag gtccatcatt
    37201 atctcctaat tcagctctaa aagcagtctc catattacaa tatggataaa ctgtatcaaa
    37261 tccatattcc ttcatttttt ctactaagta ttttgatgat atgtattctt cttcccctaa
    37321 ttcaggattg ttaaatataa aattacaaac ttcttcaatt tcttttccaa gttcattctc
    37381 tatctcatag gctcttttct ttatactctc tatcataact tacctcccac tagaatccta
    37441 gatataatcc tattgtcaaa aataccatta caagaattgt taatactacc attaaaggaa
    37501 ttacgaatct aacccacttt gaccaaggaa tattggctac tgctagtgct cccattaaaa
    37561 cacctgatgt aggtgttatc aagttagtca cacctatacc aaattgataa gctgtaacta
    37621 tcatttgtct tgatacatct actaagtcgc caagtggtgc aagtacaggt attgtaagtg
    37681 atgcaagccc tgatgctgat ggtattaaaa atgctattaa tccttctatt aagaaagtta
    37741 aatttataaa tatagttttt ggtaatccat ttaataaatt tgctgcagaa tttaaaattg
    37801 tatctattat aaatccattt tctgctatta ttacaattcc acgtgcaaat gctatacaca
    37861 atcctgctgt cattaaatct cctgctccac ttataaatga attaactata tcatcttgtt
    37921 taagacctga tgctatacct gatattatac ctatagctgt aaatatcatt gcaatttcag
    37981 ttatatacca tcccaacttt aaaactccat atatcataat cgccatacct attgtgaaaa
    38041 ttgctattac catggcttct ctttttgtaa attcctttat atttgtatca ctattaacca
    38101 ttgattcttg atttagtgat atatcgtgta ctaaagattt tttagggtct tttttaactt
    38161 tattggcata catcataaca aatcctatag atatagccat atatattata aacataacaa
    38221 ctctaaactc tattccagaa cctggtgcta tttgtgataa tgcttgtgct attcctacag
    38281 agaaaggatt cgtagtagct gctccaaatc cagctgttgc tcctataaat actgtggcca
    38341 ctgctgtcaa tgaatcatat cccatactgg tcataagtgg aataaatatc atataaaaag
    38401 gtagtgtctc ttcactcatt ccaaacgtcg tcccacctag tccaaataga atcattgaaa
    38461 ttggtattat taaaaattga aacttcttca ttttatttac tgtatgagct ataacagctt
    38521 ctatagctcc tgttttatta actatcccaa aagctcctcc aactattaaa acaaatccaa
    38581 ctacttcagc tgcatctata aatccgttta taggtgctgt aaatacatca gtaattcctt
    38641 gtggatttga tggtgctttt tcataagttc cagctacaac aactgacctt ccgtctatat
    38701 ctactctttc aaaatttcca cttggcacta tccaagtaag aattgccatt aacactatta
    38761 gcaaaaatat tattgtaagt gtctgtggca tagtaaactt tttctttttt aaactgctca
    38821 tttttaatct ccccactttt taaattaaat attttattat tgcatccctt tatcctattt
    38881 tgtatagatg taaaaaagct caacatggat acatgttgag ctaaatgtgc taaatacaac
    38941 atacctgcat cttcccttta tcttgtttaa gatagctctc catcaaaatt cgggataatt
    39001 ttgatgacag tactgtacct attaaataca gtcccagcac tagcttcgga cactagcact
    39061 tcggcactta ctccttttat attgcctcat tggtttccaa cctcttcaat attaatctta
    39121 gtaaatgcaa cctctacctc aatctgtttg ttagatgagg taattcttat taatttttat
    39181 taattcaaag taattgtagc ataatatgtc gagattgtaa atattttcag tataataatt
    39241 cttactttat gatttcattc catttttcac aaataatttt ttctccatat aatttaaata
    39301 ctgtagtata ttttaaatac acacaaactt tccacaaatt tttaattatt ataatacctt
    39361 tataataaaa aataaaagcc atgtaaactt atcaatttac acagctttat atacattaat
    39421 ctactccaag tacagcttta agctttgatg ctattgtaat aactcgtgga ccaaatataa
    39481 cttgaactga gtttttcttt ttaacaactc cacttgcacc caattcattt acccagaagc
    39541 catcttcata gaatccatca attgaattta gctctaccct aagtctagtt gcacagtttg
    39601 taacattttt tatattatta cttcctccta aaccttctat tatagaatgt acaagctttg
    39661 tatctttatc atctacttcc tcttctactg cattgttgta tgctgtttta ccagcatcta
    39721 cagattgtct ttctctatat tctttcttac tataaagctt aacttcttct gaacttcttc
    39781 ctggtgtttt tatatcaaac ttcttgattg cccatgtaaa tattatataa gtaactataa
    39841 aaaatattgg tatcaaaaat aataatggta acgcatccac tttttgtggt tgtaataaat
    39901 taggaaacat atttctaaat gattctccca ttatagaaac attaaacatt tctgtaaata
    39961 catacgtcaa tcctgccagt ggtacatata ctacaaaata taataatggt gcaacaaata
    40021 ggaatgtata ctctattggt tccgttaccc ctaccaacat ggctgttact attgctggta
    40081 tcaatatact tattgctttt ttcttattag cattatcagc caacttatac atagctaaag
    40141 ctgctgctgg taatattgca aaatgtatta ataaacgacc tgttgtgaag ttacgagtta
    40201 tataagatag tgtatctggg tctcccattt gagctaatct tatattatta acaccctcgt
    40261 aaattgctcc acctacttcc attgttccac ctactcttgt atattctata gggaatgcaa
    40321 ttaagtgatg tataccaaat ggtaataatg ctttatccat tgctccaaaa gtaaatgttc
    40381 ctaataatcc actagaacct atccaattac caagtttaga taatgccaac cctactggtg
    40441 gccatataaa gtacattgct gtacccaata ctattgatgc tataaacatc ataatcattg
    40501 aaaatcttgg acctgagaaa aaagataatg cactaggaag ttctatgtca acaaatttgt
    40561 tatgtactgc actagctaca aatccagcta ttatagctcc aaatatacta caatcatatg
    40621 aaaagattcc taagaaagaa ccatataaag aacttcgact atatgcttct gcctctccta
    40681 atccagatgc cataaacgct tctgctgtaa ctgtatctga atttatacca tttgctgcta
    40741 acaatgtact tataactgtg tgcattccta taaataaaac aattcctcct aatgcacccc
    40801 atcctttttc ctttttagat aatccaaagg ctatacctgt aacaaagaat attggtaagt
    40861 atctcattac cataaatcct aaatcgttta ttactttaaa gaatacattt ataaatgacc
    40921 cttcttttat tagtccgcta agccctaatg cagaaacgtt ttccatattt gcaaaagctg
    40981 accctaatcc tataaatact cctgctacaa caagtaatat aacaggtatc atcattgctc
    41041 cagaaagtac ttgtaattta tccttcatag tattatttcc tccattttta ttgaaatgcc
    41101 aggctatata atgcatatat atatacttct attgctttgt ataatgtatc tatacttata
    41161 ttttcatttg cctgatgtga atttggatta tccccctcta ttgtgggtcc aaatgcaact
    41221 ccatttttta acactcttgc ataagatgca gctccttttg taaaaagttc ggcttctttt
    41281 ccaaaacctt gtttatatgc atttgataat ttatttatta attcactatc tggttttaca
    41341 tacagtaatt ttaaatcctt atatatatca actattaaat tttctttttt tccaatttca
    41401 ttaattctat tagttataaa ctcccaactt atacccttag gatatctaaa gtctattttc
    41461 atattcaatt catttttctc taagaagata ctcattatac atattgtaga aactcccata
    41521 tctacatctt ctttatatat ccctaatttt tttccatgat tatcatctgt aaagtatgaa
    41581 tttaaaatat cccttattat aacgccttta tcatttagtt ttttaatttt ctctaaatct
    41641 tttgctaaac taaatgcaca gttatatgac ctgtgtggat ttctcgataa agccctttct
    41701 cctgtatatc ttactaatac tttaccttct tctagttctt ctatctctgt ataataagct
    41761 aatgattcta ctaaatcttt cttatcatta gttttaaaaa cagcctcaat tttgtcacat
    41821 acaaatccat cttctttgtt tgactttata tcaactaaat tgtgatttct aaatttatct
    41881 ttatctattt tctttcttag attgaagtat aaaatacctt tttctccatt tactattggg
    41941 aagtttccat ctggagaaaa tgcaaatttt ggctgttcat tgacactaaa gtaatgctcc
    42001 atacattccc aagtcgtctc ttcagctcca cccactatca atctaatctt tctcttcggc
    42061 ttttcattaa gttctcttaa gaaaagtaat gcatataaaa tccctatcaa aggtcctttg
    42121 ttgtcattaa ctcctctacc ataaagatag ttatcttttt gacatacttt aaaaggttta
    42181 ctcttccaaa gttctttatt atatattggc accacatcta tatgattaag cacccctact
    42241 acttcttctc cttctccata ttctatatga attgcatatc catcaaagtc ttttacaaca
    42301 aaatctttgt cttttgctat ttgaataagc ttatcgaagg catttcttat ttctatccca
    42361 aatggttgat ttatatctgt tgtactttca tctctaattg aaggtatttc aactaaactt
    42421 ctaatatcat ttatgatatt ttcttgattg gattttaaat aatcaataat tagttctttg
    42481 tttactatca tcttaattat ttatcacccc tttatattaa ttttacaaag catttcagtt
    42541 catatctaca ttataaattt tgaatattat tttttcaata tttcaaatac ttctctaagt
    42601 taccataatg atacggtaaa caatcaacag attatgacaa aatcatacta tacctattac
    42661 taatatttta acttatctat tacgtctttt ttaatgtaag tttattccaa aaaatacata
    42721 aaaaaagatg tagccttagc tacatcttct tatatctatt caacttccat tattatgcta
    42781 ttaatatcta catttccagg ttctatccta tcttttactg ttctcttaac agaatcagca
    42841 tcatccatta taactacagg agttattata tttaaatcat ttgacgttat aaaatctaaa
    42901 tctacttcca ttattaaatc tttagctttt accttatctc ctgcctttac cttaacatca
    42961 aacggctttc cttctagtga cattgtctct aaacctacat gtattaatat atttgtatta
    43021 tcattactct taatcattat tgcatgcttt gtatcaaata cattgactat ttcaccatca
    43081 acaggagaat ataacttacc ctcacttggt attattgcta ctccatctcc catcatcttt
    43141 tgggcaaaaa ctgcatctgg aacatcttct aagttcacta aatttccctt aaaagggcta
    43201 tatattttct caatcttatt ttcttttttc ttaaatatat taaacataac acactctcca
    43261 cattataaaa ttttatgata atatttttct taaagctttt gcgacaccat catttacatt
    43321 agtatcagtt acaaaatctg ccatagattt tacttcatct gtagcatttc ccatagcaat
    43381 accaagacct gcaaatttaa tcatggatat atcattttca ttatcaccta tacaaataac
    43441 ttctttgcta tctataccta atatatcaca aaatttttta actgcaatac cttttgaagt
    43501 acctttactc ataacttcaa agtttatctt tccagaactt actacttcta aatcttcttg
    43561 ttttttaaat tcttcctttg cttctctcaa agaatctacg ttttctgaga aaagtataca
    43621 ttttaaaatt tcattatcag ctttttctaa taaagtttca caacttgcat tttctattat
    43681 ttcaattctg tcttcttctt tatttttact atttattaat ctatatggat gttcttcacc
    43741 tatttcattg ttggatataa ctattttaca gcctttaaaa tgtttgttta aattatactt
    43801 ttcacctatt gtatagattt tctttagagc ttctttgctc aaaaccttct tatactcata
    43861 tcccagtaat ttaaaatatg tcccattagt tgatataata tatatatcat cccctaaaac
    43921 acttgtaaaa tatttcatag cattataagg tcttcccgta actaaggcta ccttgatacc
    43981 cttttccatg acttctttta gtgctttaat attttcttct gatacttcaa atcctttccc
    44041 catcaaagta ccatccatat cagtacaaat taatttatat cgcatttgta tcacatccta
    44101 gtaagatttt tctaatattt caacaaggtc ttctttcttt ccaaaaggta aaaatcctaa
    44161 aactggacag cttgttattt gttctaattg ttcctttgtt acattaaact cagacatttt
    44221 tgtcttaagt cccactctct ttaaaaaact ttctatttca tttcctaaag ttttagctgc
    44281 ttcatcatct tccacttttt ctaattcttg gtttagcatt ctcgctactt tggcaaattt
    44341 ctctatatta tccttataga acttttctgc aaattttggg aacactatag ccaaagcctc
    44401 accatgagga atatgtgtaa ctcctcctat tatttcactt agtggatgtg gtgctgctgc
    44461 tccagcattt gccagtgaac ttcctcctaa tgtatctgct actgctaact ttgttctgta
    44521 ttctatatta tttttatctt ccattacttt aggtagatag ttaatgacta attccataga
    44581 tttttcagac attatctctg taaaatcatt agcatttgga tttatataac tttcaaatgc
    44641 atgagtaaaa gcatcaaatg ctgttgatgc agtaattctc tctggcagag ttaacataag
    44701 ctctgggtca actatacatt cttttgagaa acaattttga tgaaatattg tatttttctc
    44761 gcttcctctc gatattactg ctgcttgtgt aacttggctt cctgttccag aagtagttga
    44821 tacagctatt aatggtaatt ctttttcact taccttatcg tatattttaa atggatttgt
    44881 atatgtacta aacatataat cccaatctat cttttctaaa ccaaaagtca atgctaatac
    44941 ttttgcagta tctatacttg aacctcctcc aacagctaaa acaacatcta cagattcttt
    45001 tttagctaat tcaaaccctt catttattaa ttctacagtt gggtttggct ctactctatc
    45061 aaagtgtaca acttctatac ctttttctat taaattcaat gtaactcttt tatataactt
    45121 gtctaatggc tctacatttg gagtagtaac taacaaacat ttctttccat attttcctat
    45181 tattctaggt aattcactta aagttccttg tccaaaattt attcttgatg gttgataaaa
    45241 gttaatcata tttttcctcc cttaaattat tgccttcata ttcaaattat acatatcata
    45301 ggatttttaa ggcaatacct tcactgttaa ctcttattta ctaaagctta tggcaattat
    45361 tagacaataa atgttttatt attaccatat cattatgtaa attttattaa gtacccatgt
    45421 tataatttta taatcaatct tattgtataa ttgacttaat aataaaaata gagtatatac
    45481 acttttttat gacaaatagc ttaaaggaga attaaattat gaaatatttg atagacagtg
    45541 ctaatcttga tgaaattcaa aagtgtttaa agtatggatt taagggaata accgctaatc
    45601 catctatgta tcttaaagag aaagtaaact tccatgaatt tattgagaca tgtgctaatt
    45661 taaatccaga aatattaact gctgaagtca ttggaaataa tttatacgaa ttaattaatt
    45721 catgtgataa gatattaaat ataaataaaa acactataat aaaaataaat ttctctgaag
    45781 ttggcttaga acttataaac atattaaata gtaaaggtgt aaaaactgca tgtaccttga
    45841 ttttcaatgt aaatcaagca tcattagcta taaatgcagg tgctgattac ctattccctt
    45901 ttataggtag aaatgatgaa aatgggaatg atggtataaa aaaccttaca gaaatatgta
    45961 accttatatc aataaatgga tataatacta aagttgttgc agcttctatt aaaaatgtat
    46021 tccacttaac tcaagcatct ctttgtggat gtgattatgc agctgtaaca tatgatttat
    46081 ttaaaaaagc ttcttttaac cagcttacaa tagatggtgc caatactttt gaaagagatt
    46141 ggtccaaggt taataaatag taactaagat ttaggagggt ttgcattgaa caaaagatta
    46201 ttagatattg ctgaattatt actcaacagc tcagactata ttactgtaga tactattgcc
    46261 gaagaattaa aagttagtaa taaaactatt cgtaatgacc ttgttatttt agatgaatgg
    46321 cttttagagt ttaatttatc cctagataaa aaaactggtt caggggttat tattctaggt
    46381 aatgaagata taaaacttaa agttattaga gatatcaatg ataaatcaaa ttgtatatat
    46441 gcatactctc ctgaagatag aaaaaggtat attctaagta aattatttat taaaaattct
    46501 aagtttagga ttcgtgatat ttgcaatgaa ctacatgtta gtagagctac tgtgcataaa
    46561 gacttggtag ttattcaaaa ttttttgcaa aactttaaag tgactctagt tagaaaaacc
    46621 aataatggag tatatttaga gggaaaagag aaagatattc gaaaagcaat ttttgaactt
    46681 gctacggtta ataaaagtta tactgaactt aaagaaatat tattctcgaa atctaatgaa
    46741 tgcaaagata ccccttcaac aaaaatattt aaggaactat ttaactatga ttttgagaaa
    46801 ttatctcaaa tcactctaca caccttacaa gtagaaaaaa gtaaccttag tgatgagtat
    46861 tatataaact tcttaataca tatagctata tgtattaaaa gaatttcaat taaaagatat
    46921 attaacttat ctgaaggttt ttttagagaa ttattagaac atgaatactt tgaaaaatca
    46981 aaaattttat gtgctaacct aagtgaagct tttgacgtag aatttattga tgaagaggtc
    47041 tgttatatcc ttcttcatat agaaggatta cttaaatcaa taaagaaaac tactactctt
    47101 gaacctctta aaaatgaaga aattacagag tataatctat ctgaagcaat agctgttcat
    47161 tggggaagta tactaaactt aaacttaaag gatgatttaa tattaataaa gtctcttgct
    47221 aatcacctta aatcagttct acacagaata aactatgggt ttactattac aaatcccata
    47281 ttaaatgata taaagagaac atttccttat acttataagg ctgctaaaga gtctaatact
    47341 gttatcaaac agttgatgaa ttatgaagtc aatgatgacg aaataggata tcttgcttta
    47401 tatttaatat cagcaataga tagaagcaaa tctcccctaa atactatttt aatatgtcac
    47461 tgtagtgatg gtgtatctaa tttattagtc caaaagttaa gttttgaatt taatcagatt
    47521 aatatagtta aatctatacc tctatcttct atttcattta cgaactttga tgatgtggac
    47581 ttaatactaa ctactgctcc tgtagacttt gaacataatg ctgaactgat aaatataaat
    47641 gctcttttaa gtaagaatga tatttctaga cttagtacaa ttataaaaaa actatattct
    47701 gaaaaaaata aaatattatc ttataaataa acaaaaatag gcttgtctca acatagagat
    47761 aaacctattt ttcattgtct aaatgataaa aatatatttg atttcataga aaaagtgcct
    47821 catgaactaa aaaattcatt ttgagacacc ttcatttttt atatttaatc tttttattta
    47881 agcttttata ttttttaaac ttttatattt aaagtaaatt ttattatttt tattgagcac
    47941 aagcattatt tatattatta atttcttcag tattcttacc aaactttgta gttacaaatc
    48001 ctattgtttt tattatgagt aataaggata tcaatgaata tatagatatc ataaaattct
    48061 tacttattat tccagacgct actattataa atccatctag cataaataat ataactggca
    48121 acttcagaaa cttaattacc ttattaataa gcattgcaac taaatcagtt ccaccagtac
    48181 tacagcccct tattattgct atccctatag cagttcctac caataaccca cctatcactc
    48241 ctgctactaa tagattgtca gttatgtgag tttgtggtat tacttttaag aataatggac
    48301 ccataagagt tatatataat gtttttattc caaaaccttt ccctaaaaat aataatccca
    48361 ttactaaaag gggcatagat attcctaatg atatattctc aacaggcatt gataagaatg
    48421 tgctaagtac gactgataat cctgttatcc ctcctggagc taatgtatgt tctgcaaaga
    48481 aaaaattaac tgctaatgcc attaaaaatg ctgatattgt tatcagtaaa taatctttta
    48541 tcatttcttt tttcatttaa taacctccta tgattatatt gtttcagtat aaaaaatcaa
    48601 ttaaattagt ctagcttcct gtagtttttc ataaaattaa tgcttcctta aaacattaag
    48661 aaagcatctc ttttatcata gtttattatc ttattctaaa ccaaactttg tagctgcttc
    48721 atctatagct tttctacaat tcttaacacc tgctgttata ccatctttat ccttgaatat
    48781 tgctgaagat aatattacaa cgtctggatt acattctatt aatggctcca tattgtctgc
    48841 tcttagtcca ccatctattg ctaattcaca cttaggattc ttttcgtcta tcatttttct
    48901 agctcttttt actaaatcaa cagcactctt tctccatccc cagttatctt ttccatcagt
    48961 ttcatcaaca ccatgaacga ctatttgcaa tctatctata tcatatatag cttcatcaac
    49021 aaaacataat ggtgtgtaac atccaattgt taatcctact ttcataccaa attctctaca
    49081 gtaatttatt atgtatgcaa gaggagcacc taaaaaatgt tctgctggta ttatcaacaa
    49141 gttacatcct gctgctgcta ttttttctat aaataatcta tcacaatctc ttgtgtatat
    49201 atggcattct attggtttat ctgtcactgg tcttattgct tctattattt gatgaccacc
    49261 cataagttgc atatttttta agtcatgcat atctgctgca tctgaatgta cgtagtctac
    49321 acctgcatcc attgcttctt taactacggc tgatatatga ccgtaattta catgtgctaa
    49381 tcctgctgct atttttatgt gtttcatatt tgtatacctc catattttac tttaattagt
    49441 attaaatatt agtttttgat atttttttaa tattttttaa tccagtatta cttttattta
    49501 gcattacttt tattgctgta tatatttctt ttatgaactt attctgcttt tgttaactgt
    49561 gaacttattt tgctttgtaa attaatatta cactgatata ttttttttca caacactttg
    49621 actactcctt aaaagtacct ttatattgtg gtaaattata gacattaatt gtatataaaa
    49681 agttagtttt ttaccacatt cttatggcat ttattgtcta tactaactca attgatatag
    49741 ctagttcatt taatatataa tcagactata aatataaatt tactcgataa aaagtaccat
    49801 tcataacaat attaatgaaa gggagatgta aaatgagaaa ctgtattatt gcccaatctg
    49861 gaggccctac agctgttatc aatgcttctg cagttggtat ttatgaaaaa aatttagaaa
    49921 ctaaatactt tgataaagta tattttggta tcaatgggat tgaaggtata ttaaacaaaa
    49981 atataataga ttcagactcc ttaaacaaag ataatgtatt aaatttaaag caaacacctt
    50041 cgtctggtct tggttcttgt agatacaaac tggctgatta taaaataaat tcttatgatt
    50101 atgatagggt tataaatata ctagatgaat atgaaataga tacattattt tatataggcg
    50161 gaaatgattc tatggacaca gtgatgaaat tttctcaata ctgcaaaaac actacttgta
    50221 aaataaaatt tataggtatt cctaaaacaa tagataatga tttattaaga actgacaact
    50281 gtcctggatt tggttctgct gcaaagctta tttctacact aataatggaa acatacttag
    50341 atttttctat atacaaaaat aatggtatat ttatagtaga aactatgggt agagatactg
    50401 gatggttagc agcttcttct tcactagcta gacttaatga tactccactt gttgatttaa
    50461 tataccttcc agaaattgat ttctatgaag ataagtttat agaagatgtt agaaaaatat
    50521 ttaaagaaaa aaataaggct tatatagttg catcagaagg tataagggat aaatacggca
    50581 actttatatg cgaacaaaaa acttctgctc atgataaatt tggtcattcc caattaggtg
    50641 gagtaagcaa ctatctaaag aatcttataa tatcatcatc aattacaaca agagtaaaaa
    50701 ccttagaact tggtgtaata caaagatgtg ctatgcattt agcttcagat actgatttaa
    50761 aacaagcagc tgaagttggt gcatatggga tagaactatc taaaaaagga tatacaggag
    50821 ttatggctat cattaataga acatgcaata atccttataa gtatgaagtt agtcatactg
    50881 atatttctaa aatagctaac aagacaaaat attttcctaa agagtggata aatgaagaat
    50941 gtaattatat aactgatgaa gccctaaatt atctatatcc acttattcaa ggtaaaccag
    51001 atatcaaata taaaaatgga ctaccttatt tttcaattat ttactaggaa attattataa
    51061 tttgatatac actcaattct atcgctctta aatacctaag tggctgttaa ataaaattat
    51121 aaattgttat atgacagtca cttaatcgtt taattattta actgtttagt tttttaattt
    51181 tttaatcttt ttaatactaa tacaatttgt tgtaataata tctcacaccc attttcattt
    51241 tggatagatt ataattagtt agacaataaa tattttgtca tgatattatt atatatagtg
    51301 atacaaatag aaatactttt atcatggcta gaaaaacaaa gattacacaa aggatttaaa
    51361 aatcaaacaa cttcaattaa aataaatggt taaaataact tctaaatcaa ctacataaac
    51421 aataaaagaa cataaagtag ttaaatctac aattataaaa ttagtaagac tataataact
    51481 taaataaaat taaagttaac aaagactatt aaagttagca aatattcctc tacctaaata
    51541 attgcttttt ataaagagtt atttatttta attgaattta attgaaatac gattttaact
    51601 agaacaatag attaatgtaa tatttaatat aaaataatta taataattga aaaggaagta
    51661 atctatgatg aaaatagcag tcataacaac cgaattttta aaagagtttg tagataattc
    51721 tataaaaaag ttaaatatta atgcagaaat tgaaatatat atctatagag atttttctca
    51781 tgttggagat ttatatttag aaatagaaga taggtttgat ggatttgcag ttagtggccc
    51841 cattcccaaa aaagctatta caaaaaaggc tggaactatt aaaaaacctc tagttgattt
    51901 tggtactgac ttacaaagtt actatgatat gtttctaaag cttatcttta aatataatac
    51961 attagatttt tctagagcgt actttgactt aacagactgg gcagataatc caaaagatat
    52021 atcatattat ttatcaaatg gcacatttgg agacttaatg gatagtattg atgaaaatgc
    52081 atctaaaatg agtttatctg acatagcaaa acttgaaaaa aagattgcaa aaaaacatat
    52141 taatctatgg aatgaaggga aaatcgattt ttctactact cgttttagta gtattgttcc
    52201 tatgttacaa aatgcaggag taaactttca tttcatatac cctgatatag atttattaaa
    52261 aaatactttt aataacttaa taaaagatat tcatttacat agaatgaagg gaaatcaacc
    52321 aagtgtaata tatataacag ttaaagatac aaattatgat gactctactc tagaccaagt
    52381 ttataaaata ttaaatagta ttaaaaaaga taaattaacc gattttataa ttgaaaaaaa
    52441 tagtaattct tttaaaattt tcactaaatg tgaaacgatt aaatcattaa ctaaaaattt
    52501 tactgcatgt tatataaaag aatctattga aaatagtaca aatattaaag tatgtgttgg
    52561 atatggaatt ggtaatgata taaagcaagc tatgtctagt gccataagtg ctaataagga
    52621 atctaaaata aatcatttaa attacagttt cttagttaac gaaaattatg agacaatagg
    52681 acctttactg tgtgataagt tacttactgt ttcaaataaa gtaactccac atataaatac
    52741 aattgccaaa agttctaaat tgtctactct tactattcaa aagattatat ctgttactga
    52801 aattctacaa agagatgaag ttactcctaa ggaattatct tcatgtttaa atgtaacagt
    52861 tagaactgct aatagatttc taagtagtct cttaaagtct ggaaacgcta ctatattata
    52921 tgaaaaacaa aactcaacaa aaggtagacc tgaaagggta tacaaattat ttatatcaga
    52981 aaataaatat taaaatccaa aatgcataaa aatattttat catctaatat taagtaaaat
    53041 tatatatgac tttctaaaag gtatatcttt gcatcttttt aagatatacc ttttatgata
    53101 tttaataata tttaataata ttttatgtta ttatctacta aaattatttt ctaaccattt
    53161 tactgcacaa tgagcaaata aagctgaacc ataaactaat ccctcttcat taaaaattgc
    53221 tttaggatta tgcataccaa aattatatcc ctgactaata cttcccattc caatccataa
    53281 aaatactcct ggaaccctta gtaaaacctc tgaaaaatct tctgaaccca ttgcagatgg
    53341 aaagtctatt acattttcat ttcctataac ttctttaaca taagatacaa tctcatctcc
    53401 tacatccaaa tcatttataa ctgcaggagt ccccatttta aactccactt tagcatttcc
    53461 tccaaatgat ttagctattc ctgtagatat ttcaataatt ctatttttta atttttctct
    53521 aacaaattgg tcaaatgttc ttaaagttcc tgacatagat gctttatttg gtatagcatt
    53581 tggtacgttt cctgcattaa acataccaat agttaaaact acaggttctt tctgaggttt
    53641 ttctcttgaa agtatagtgt ttaatgctat ttgtatatgc gacattatgt ttattgggtc
    53701 cactccaaat tcagggcttg ctccatgagt tgcttttcct tctactgtta tttcaaatat
    53761 atccgcagat gccatagctg gaccacgtga ataagctact tgacctgtgt taagaaattt
    53821 acccaccata ctgtgaaatg ccatagctgc atcgacctga ggattttcta atataccatc
    53881 atcaatcatt gattttgcac ctctggtagt ctcttcatct ggttcaaata caaactttac
    53941 tctaccttta agtaagtgct tattatccat aagtatttta gctgctccta agagcattgc
    54001 tgtgtgatta tcgtgtccac aactatgaga tgcatcttta ttcttacttt taaaaggtaa
    54061 atccgtctcc tccctaatag gaagtgcatc catatctgcc cttaataata aggtctttcc
    54121 accattttca ctgtctaaaa cagctactat tccattatta gacgtcaatt tcggagaata
    54181 tcccattttt tctaaagtac tggtaacaaa ggttactgtt ttcggtagat tgcaaccaat
    54241 ttcgggattt tcatgtaaat accttctcca tctaataatt tccccttcta ttgactgcgc
    54301 ttctttaaaa aaatttttca tattccttcc tcctaatcag atagtcagca aaatctcact
    54361 ttcttttagt aatgaaatga tgctgatata aaaattataa ttttatatat taaatttcat
    54421 gtctttttaa ggaattggaa tatcatctgg aataggacat ttatatgttt taccattcat
    54481 agatttatca aattcttctt ttgccttttt agctatctct ggattattta atattttagc
    54541 tccaaacata gccatgattt ttgatgcata tatcatacct ttttctccta tagaacttcc
    54601 tgcacaagat gtaaactgcc agctatgacc tggtgctcct agattcgtac accctgtaaa
    54661 aaagtatgct gtcggaacta tatgcattac atctccaata tcagtagagc cataactatt
    54721 aaagtttgta acatttcctc caccataata tagatgagtt ccttctggca aattatattt
    54781 acttatcata gccttatatt gttgtgggct ttgtctatct aattcagctg caaaatccaa
    54841 ttcctctcga gtccaaggct cttgtttaat ctcattcata cattctgata caagatttgc
    54901 taaaacatga ttgtttaaag tattataaca tcctcctaaa aattctactt caacttcagt
    54961 ttcagtcatc attgcagctc cttttgctac ctttacaagt ctttcatatg tactttctac
    55021 agcttctcta cttaaagctc ttgtataata ccatactgat gctttatcag gaactatgtt
    55081 gggtgctact cctccatctg taattgtata atgaatcctt acatcagaag taacatgttc
    55141 tctcaaataa ttagctccca cattagtaag ttccacagca tctagtgcac ttcttccatt
    55201 ttgagggtct gaccctgcat gtgcagtctt tcccttaaag tgaaacttta cagagttaac
    55261 agctgttgaa gtaccaattg tagcttcact aattttattt ggatgaaaat ttatggctaa
    55321 gtctaaccct tcaaatgctc cacctctagc cataaatcct ttacctgtta attgttcttc
    55381 tcctggacat gcataaaata ctatagtccc acttaagtta ttttttatca tctctctttt
    55441 aagccctact actgccccta catgtgctgt acataataaa ttatgcccac atccatgacc
    55501 atatgcaccc aattcaaatg cttcttgctt tgttgaaact ttttgattta aaccaggtaa
    55561 tgcatcaaat tctcccataa aacctataac tggtttacct gaaccaaaac tagctctaat
    55621 agccgtggct aaacctccta cacctacttc cacatcaaat ccttcacttc ttagaaagtt
    55681 tgctgtattt tcacatgctt taaattcttt aaatgctttt tctggatttt cccatatttt
    55741 cttacttaaa tcatcaagtt tgaatctttt tttatctatc tcatttatag atatacttcc
    55801 tatcattttc tattcctcct ataaatctat ttactatcaa ccaaatataa ttggacctat
    55861 tatactagca aatgccactg atgcaatggt tacagtaaca aatcctccta taatcatctt
    55921 tggtaataaa taatctgttg ccctttgttt ttcttcttca gttccttcca tagcatcaat
    55981 accttcattg gaaattatct gtgttgcagg ataagctagc atacaagtta caccaattgc
    56041 agctgacatt cttggagaat accccaatag ttttcctaca attgcagtaa atagtattat
    56101 aaatacagag cctacaacaa gtattcctat agttggaaca atcatcccaa acaatgctgt
    56161 tggagttacc ttagcaagtc ctccaggtaa catcaacata caaccaaaca ttataattcc
    56221 attagattga gatttatcta aaattccttt ttctaaaaaa cctattcgtg taaaaatcaa
    56281 ccctactaac aaacatgcta tattaggatt taaataatag tttgcagggg taaatcctgg
    56341 tattaaagta gccaatccta caaaattacc aatcatagca acaaatgaaa gctttgcaag
    56401 atacactaga gtagatgatt ttttacctaa atcttcttta aaaattctca tacttggaag
    56461 tttaaaatta ctttttgact catctttcaa aaaatccctg ttatctcttt taaattttaa
    56521 ttctttattt atacaatatg tagttatcgg aataccaaag aacttttgaa atgataatac
    56581 tagaattaca aatgcagcaa gttcaggtct atttgcagct tctgcaacag ttgttacgat
    56641 aatcccagca acagtagaac ctgctattgg aggtgctgct attaaagcat actctctgcc
    56701 aaataacata ctacccactg ttaaacagcc aactcctatt cctacaatac ctactaatgc
    56761 aattacaacc gttttccatt catgacataa gtcttcaaga tttataagtg ttcctaagtt
    56821 agttattaat aaagctatac caaagttgct cattgcagct aatatccctg atgctcctgt
    56881 aatgtctttt ggtaaaatat ttgtccaaaa tcctagtaaa aatataatac atacaaataa
    56941 caatgttgaa attgcacctt ttgttttctt agataaaaac tcactaaaag cataaatcat
    57001 tacacataca gtaaagcaag taagtggatt ccaaaaattt tccattgttg tttcctccta
    57061 aatataataa ttttttatca ataaaacaat aattataaaa tgtatattat gcagttattc
    57121 ttaactacat atggtatagc ttgatatttt ttgatgatat tatatacgtc ctaaattatt
    57181 cctaaaatat ctaaataaat ataactagag ttaactctct aaatattaac tatagttatc
    57241 aacttactag atttcttttt gatttttact ttctacataa ttttacgagt tagaattgta
    57301 cttcaactac tctctatatt agtatacatg ttggaagact tatatttatt taaatattag
    57361 atatagaata catttttgca aacgtcttaa attattctta aaatatctaa aacgaaataa
    57421 taatataccc attaaaccaa tattatcaga atatacttca ctttaagtct tctttatata
    57481 gaaattttat ctattagtgc atcccctcct ttgtaaatta gactatttct aaacgaattt
    57541 agatatctta ttcctaggaa ctattactag aaacaaaaat tatttattat attaaatcaa
    57601 tgataagcca tactttaaaa ttctttctat acaagtatga tttatcatat aatgacatct
    57661 atacctttat ctttatagta gtcctaaatt attcttaaat tgacaataaa aataatttca
    57721 ctatttgtaa aaatacttgt ttttatattg tcaaactatt cttaatatag attataattt
    57781 taaatctttt ctttgtcaat atattctgac atttttccag aaaatttata aagttatata
    57841 gtaagtaaat cagactattt ttatataatg agtaaattat actatgaata tgtctaaata
    57901 ttgacttaag tataaacata aaaaatgtgt attctccttt ttatagtaat tttagttgcc
    57961 attactttta agaagaatac acactttact ttaaagaccc tatactctat tttaaatgct
    58021 ttactagaca ctaattaaca ttagttccta acttctattt atcttattcc taccctacta
    58081 ctatatccgt agaaaaatct aaaacttctc cattagattt tattacttct ttgtaccaat
    58141 caaatgattt tttcttatat ctcttaaaac ttcctttacc ttcatcatct aagtcaacat
    58201 atataaatcc atatctctta ctcatctgac ctgtagttgc actgactaaa tcaattggtc
    58261 cccacatcaa atatccaaaa cattcaacat tatcttcttc aattgcagac ttcatttgag
    58321 atagatgctt acttaaatat tcaattctat aatcatcatt tatagttcca tcttcttcga
    58381 ttttatcata tgcccctaaa ccattttctg taattaagat aggtaattga tactttctgt
    58441 aaatatagtt caaggtaaat cttaaaccta tagggtctat tgcccacccc cacttacttt
    58501 gttccaaata tggattttga atcccaccaa ataaagattt atcacctgat aaattttctt
    58561 ttggagtaga tacacttgaa aaataataat taagtcctat aaaatctaac ttaccttctt
    58621 taaatgcttt tttgtcttca tcagtcactt caatagttat attatttttt tcatattctt
    58681 ttaatttata ttgtggaaac ttcccattgc acattgcatc aatttgataa taatcacggt
    58741 cattatctaa aaatgcattt aacacattac taggattaca attgtatgga taaattggat
    58801 ttagtccaaa tacacatcca actttattgt tgggattgat ttctctagcc aactttgcaa
    58861 cctttacacc cgccaaagtc atattatacc ctacatttgc caatgtttga accttatttt
    58921 ctatttcact atacttaata cctgctatca tatacgtaaa aatatccgag tgctctgttt
    58981 gagggtcaat atggttcatc tcattaaatg taacccagta ctttacctta tcatcaaatc
    59041 ttcttattac agtctcactg tactttaaat acaaatctat aagttttctg ttattccatg
    59101 aaccatattt atgtactaaa ttcataggta attcaaaatg atatagtgtt acaataggtt
    59161 ctatattatt ttctattaat gtatctatca gaccttcata gtatttaatc cctaattcat
    59221 taggattttc atcatctcca ttttgaaaaa ttcttgacca atctatagat attcttaatg
    59281 cactaaatcc catttcttta aataaagcta tatcttcttt atatctatta taaaaatcta
    59341 ttccattatg agatggataa tccaattttt cttctatttt atcagtaatt actcttggcg
    59401 tttcataaga gcccttagtt acaaaatcca taattgcagg tcctttttta tcactatccc
    59461 atgaaccttc acattgatgg gctgcaatac ttcctcccca gaaaaatgta tctttaatat
    59521 tcatgaattt tccctcctaa aatttatact attacagtta ataattcttc tgattcatca
    59581 tttgatgaga ttacatctaa ataatcgcta gtatttgtaa ctacagttat tatagtagaa
    59641 tcaaagcctt cctctataat tttatcagat tcaaatactg ctaaagcatc tcctttttta
    59701 accctttgat tttgtgttac aagactttca aagaattttc catctaagtt tacagtatct
    59761 atcccaatat gaattaatat ttctaatcct tcatcagtca ctataccata tgcatgttta
    59821 gttggaaata ctatagttat agttccatct acaggagata taacagttga ttttgattca
    59881 ctaggtataa ctccaacccc tttacctaat gctccagatg aaaatgcttt gtcattaact
    59941 tccccaagtg caattacctg accttttgca acatttccaa tactaatagt tcttgaattg
    60001 ttctcgttga ttattagttc taattctttt tcattatcac ccttaacttc ttctttttca
    60061 tctgaaggta ttcctagtaa ataggaagct gctgttgctg ctacaaatga tactactaca
    60121 cttattatca taaatgtaaa gttagatact tctcctccaa caagatatgt tggtaatgca
    60181 gctaatcccc aaaccattcc atatgtttta acttgagcta aacctgctat taaaccacct
    60241 atacctccac ctatcattac tgctacaaat ggtcttctat atttcataaa cacaccaaac
    60301 actgaaggtt ctgtaactcc cataagtgca ctaaaactta ctgtaccaaa taattgtttt
    60361 tgttctttat tcctagtttt taagaaatat ccaaacattg caccacaaac tgcaatatca
    60421 gatattgttg cagcgcccat aaaaattggg tcatacccca atgttgtcac agaatttaaa
    60481 gatataggca tagtaaagtt tgctgcacca aagactatta agagtggttg taaagctgca
    60541 tacagtgcaa caacactcca attacccaat gtatcaatta aaaacataca tgcatagttt
    60601 aatccatcac caatccacat accaataggt ccaaatacag ttaatgtaac tgatagagtt
    60661 attattaaag ataatactgg aactaaaaaa tagtgtagtg ctttaggtaa tatttttttc
    60721 aaaccttcaa ttataattcc cattgcccat actcctaaaa gtattggaat aaaactgttt
    60781 gcataagttg tagcctgtaa atttatacca aataaactta atccttttac accatctatt
    60841 gatgaagata ataacgtaac tgctaatact actgctatat atggatttac attaagtctt
    60901 tttgctgatg ctattgcaat taatactggt aagaagtgaa atgtagcact tcttaatgta
    60961 tctaatattt gatatgtagg actgtcactc tgaagtatgc cagacatatt taaaagtgct
    61021 aatatacttg caagtatacc agctgcaagt agtacttcaa ttactgaagt catagtctct
    61081 gagactacca caaagatgga attaaatata cctttgatac ctctaattcc cttttttctt
    61141 tcacttttta tacttgattc acttgaactt aaacctatca tagacataaa ctcgttatat
    61201 acatctacaa catgagtacc tattataatt tgataagcaa catcattaga aactacatca
    61261 attactcctt ttaatttttt aatttcttct gtttgagcga tactcctatc atttaaagta
    61321 aatcttaaac gagtaacaca atgcgctacc gactttatat tttcttttcc ccctataagt
    61381 tttattatat tttcatttaa atctttatat ttcatctgat tttgcctcca gtctttgagt
    61441 caccctattt atatgcaaca ttaaataagt ttcttcactt atagatatat cgacattaaa
    61501 ttgtgatttt atatattttc ttatcttttg aacacttcca aatgactttg gataaagttg
    61561 acttacttgt ttatataaat ctatagaatt atcttcatct attgaagctt catccgacat
    61621 aattctttgg ataaagtact gtagatgagt agtaaatctc atataatttg ttgaagtttc
    61681 atctagctct acattaaaat gatattttat tatagaaact atatctgcta aagttctcaa
    61741 ctctacaatt gtactatcat gagactcttt atttacctcc atatttacaa aatgaagtgc
    61801 tattgatact gcctcatcat caggaagatt aattccctta ctatcaccaa ttaattttag
    61861 tgcatgcatt ccaatttcaa aatgctttgg ataaaatctt ttaacattcc ataccaaagg
    61921 actttttata aattgattct tttcacatct tttaagtaaa aactctaaat ggtctagtaa
    61981 ggttaagctt agataatcac ttgactttat atttaaagct ttctcaccat attcaattat
    62041 ttcattaata agaactatat tattggagtc tgagttttgt agtagataac taaagtgttc
    62101 tagcatatcg tatgaatcta gtatatatgt tctctctatt tcattttcat ttattacttg
    62161 tcccactctt tttttaaagc caattccttt aacaaataca ataatttcat ttttacctct
    62221 ttttactaag gctacattat tatttaaaat ctgaattatt ttcatatatt cctcctttca
    62281 ataaaaagaa ctcaagcata tatgtataga gtatccctct atttttcaca tatatacttg
    62341 agttctgcct gctaatcagt aacatactca aaattatgac tacgtcgcca taattaaaat
    62401 tcttttttgt ttaatttcat attttatatg ttatactatc catataaatc tgtcaacggt
    62461 ttcctagata tttttttatt ctataactac aagctctgtt atacgattat ctgacaaggt
    62521 ttatatacta aattgatact attttaccgt cactaatatt tataatattg tctgaaccat
    62581 tatctatttt actattacga attatcatag taatgctttt aaatataatg aactgtatgt
    62641 taacaaataa attcttaaga tatattaaca tataaagtct taagtttagt tttatttaat
    62701 cactattaag atatattaat aggtaaagct tcaaatttag tt
//
LOCUS       DAEMHZ010000005        62477 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000005 DAEMHZ010000000
VERSION     DAEMHZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.005"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            96..509
                     /locus_tag="KPK72_000292"
     CDS             96..509
                     /locus_tag="KPK72_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487543.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKYGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            577..993
                     /locus_tag="KPK72_000293"
     CDS             577..993
                     /locus_tag="KPK72_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487544.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLSTKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            994..1206
                     /locus_tag="KPK72_000294"
     CDS             994..1206
                     /locus_tag="KPK72_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487545.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAVDIAKVFDTTVENLFEFIEEE"
     gene            1603..1773
                     /locus_tag="KPK72_000295"
     CDS             1603..1773
                     /locus_tag="KPK72_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487546.1"
                     /translation="MSALLYGVCNKPILISIVTNMQEFKIGVSMDYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            2316..3194
                     /locus_tag="KPK72_000296"
     CDS             2316..3194
                     /locus_tag="KPK72_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG2487547.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            3230..5125
                     /locus_tag="KPK72_000297"
     CDS             3230..5125
                     /locus_tag="KPK72_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2487548.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            5393..7300
                     /locus_tag="KPK72_000298"
     CDS             5393..7300
                     /locus_tag="KPK72_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2487549.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINNLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSESNSSYNDESDRLSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            7290..7739
                     /locus_tag="KPK72_000299"
     CDS             7290..7739
                     /locus_tag="KPK72_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2487550.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPKSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            7839..8726
                     /locus_tag="KPK72_000300"
     CDS             7839..8726
                     /locus_tag="KPK72_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2487551.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            9131..10579
                     /locus_tag="KPK72_000301"
     CDS             9131..10579
                     /locus_tag="KPK72_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2487552.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLCERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            10605..12995
                     /locus_tag="KPK72_000302"
     CDS             10605..12995
                     /locus_tag="KPK72_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2487553.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            12997..13668
                     /gene="pgmB"
                     /locus_tag="KPK72_000303"
     CDS             12997..13668
                     /gene="pgmB"
                     /locus_tag="KPK72_000303"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2487554.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            13707..15107
                     /locus_tag="KPK72_000304"
     CDS             13707..15107
                     /locus_tag="KPK72_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2487555.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDHISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIDNVVEEV"
     gene            15424..16266
                     /locus_tag="KPK72_000305"
     CDS             15424..16266
                     /locus_tag="KPK72_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2487556.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTYDELIYLTIHLAKVVK
                     DSNM"
     gene            16411..18339
                     /locus_tag="KPK72_000306"
     CDS             16411..18339
                     /locus_tag="KPK72_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2487557.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMVIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALFGIA
                     ISMVVAFVLTMILYKDEKVEKSKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            18400..19827
                     /gene="ascB"
                     /locus_tag="KPK72_000307"
     CDS             18400..19827
                     /gene="ascB"
                     /locus_tag="KPK72_000307"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2487558.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            20238..21014
                     /locus_tag="KPK72_000308"
     CDS             20238..21014
                     /locus_tag="KPK72_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2487559.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            21030..21395
                     /locus_tag="KPK72_000309"
     CDS             21030..21395
                     /locus_tag="KPK72_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2487560.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(21559..22308)
                     /locus_tag="KPK72_000310"
     CDS             complement(21559..22308)
                     /locus_tag="KPK72_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2487561.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKGTSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            22786..22920
                     /locus_tag="KPK72_000311"
     CDS             22786..22920
                     /locus_tag="KPK72_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487562.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     "
     gene            23279..24013
                     /locus_tag="KPK72_000312"
     CDS             23279..24013
                     /locus_tag="KPK72_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487563.1"
                     /translation="MSKPKKVIWVILGIIIFVFSVFLGLGYLGQITGGNSLIQRTEMN
                     DKYVPEEITKYYPIENLNSKENLLSDKNYANSIQDALLSASIEFEQGEEYKAHIDKII
                     KEFENENYKSVLYISEKNDIESSLTFSKFKIKEVDGKKRYAYITSVHEVIKKDRPYDK
                     DTMSLLKSQLALSDSLQDLNISPDNSRFLYGFVHDEDIYNTKIENKKPDEIIYFELCE
                     KPFYFWYYENFQSDKSGKSLSIEIER"
     gene            24049..24357
                     /locus_tag="KPK72_000313"
     CDS             24049..24357
                     /locus_tag="KPK72_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487564.1"
                     /translation="MKKLYCMNCGAEIKGGIPNECSCGAEFDLADTKNIKKIITSIIF
                     FVILMSPLIILIHFGRQYLQASIILYALLLILGIFWCRIVQTILVRLGLVTMTNIEIK
                     "
     gene            24809..25339
                     /locus_tag="KPK72_000314"
     CDS             24809..25339
                     /locus_tag="KPK72_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487565.1"
                     /translation="MEKILENTLLLMILPWIFVWGILCILEKRKRKNEDGNKDEKRYV
                     IQNDNIFFPLFSLGMFLFSFCLFYTVFFTEFDISEVISVSAFVIFFAILTLDSVIWRV
                     IVDGDIIKYRSFYGITRTYNFNDITKGVYTKKGSLKIYIEGKRIFTFYDSLEFSLFEN
                     QMRKRKIPLEYKRKEK"
     gene            25703..25927
                     /locus_tag="KPK72_000315"
     CDS             25703..25927
                     /locus_tag="KPK72_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487566.1"
                     /translation="MDCEMTDIIAIISSIIAVGYSIYSIVTKGYIRQYNLKKFEHFSL
                     NQKKVCIAFNFIMTLIIGISGVYILLLYFS"
     gene            26024..26398
                     /locus_tag="KPK72_000316"
     CDS             26024..26398
                     /locus_tag="KPK72_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487567.1"
                     /translation="MLILGIIVVIVSLYLLYLKYRVVFTGEKCKGKIIGIVDQNAGYV
                     VGGAFVKKHAYIIKIKNKKYYTAHGCILLSLGKRKIGKEIFVFKNEKYGKEVFKCFDF
                     RIEIVTLLTCLLGVFLIYESLQ"
     gene            26530..27744
                     /locus_tag="KPK72_000317"
     CDS             26530..27744
                     /locus_tag="KPK72_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2487568.1"
                     /translation="MNDKNFIETLRQKREEYGVTQTRIAVACGISREYYNRIEKGKQP
                     LNDELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQLESYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDRAGILDIPKLKEKYMAGECVSYFRKQKNYGSTEKCGDDMPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKIGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNEDWAWFVGDNREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMIQALDRENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            28502..28747
                     /locus_tag="KPK72_000318"
     CDS             28502..28747
                     /locus_tag="KPK72_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487569.1"
                     /translation="MELLWAMTKAVELLRKDFYRKGVCVIADIVKTRQEKTNNKRSIG
                     NRGKTKIVVKEHFSEKGKTMEELLTDVMLEKAKQTIA"
     gene            28859..29035
                     /locus_tag="KPK72_000319"
                     /pseudo
     CDS             28859..29035
                     /locus_tag="KPK72_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(29100..30776)
                     /locus_tag="KPK72_000320"
     CDS             complement(29100..30776)
                     /locus_tag="KPK72_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2487570.1"
                     /translation="MLQADKITALYCRLSQEDMQAGESESIQNQKLILQKYADEHHFF
                     NTRFFVDDGFSGVSFEREGLQAMLREVEAGNVATVITKDLSRLGRNYLKTGELIEIVF
                     PEYEVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAKAQRGERVNG
                     EAPYGYLIDPNNRNHLIPDPETAHVVKQIFAMYVRGDRMCEIQNWLRDNKILTVGELR
                     YRRTGSKRHPRPQLNAWYNWPDKTLYDILTRKEYLGHTITGKTYKVSYKSKKTKKNPE
                     EKRYFFPNTHEPLIDEETFELAQKRIATRHRPTKVDEIDLFSGLLFCADCGYKMYAVR
                     GAGTPERKHAYTCGNYRNRARNDMLCTTHYIRKSVLKELVLADLQRVMSYVKEHEQEF
                     VETANECSAKAIQKTLTQQRRELDKAQSRIDELNILFRKLYEDNALGKLSDEQFAFLT
                     SGYDEEKKTLTRRIAELSQEIDTATERSADVKRFVVLVRKYTAIEELTYENVHEFIDR
                     ILIHELDKETNTRKIEIFYSFVGRVDTGDKPTESISYFRQIGADVKSYAI"
     gene            complement(30877..31044)
                     /locus_tag="KPK72_000321"
     CDS             complement(30877..31044)
                     /locus_tag="KPK72_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBG2487571.1"
                     /translation="MNGNHAAVVTTEHKIGKTTYFVCSSSSERATDTLDKKIKKLIRK
                     DLENNAGNARK"
     gene            complement(31509..31769)
                     /locus_tag="KPK72_000322"
     CDS             complement(31509..31769)
                     /locus_tag="KPK72_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2487572.1"
                     /translation="MNGTNGNQPGYPENALVPYPVIVAATKGDPNAMKMVLQHFSGYI
                     ARLSMRKLYDERGNVYFGIDHDIRERLQAKLMMAVLNFRTEE"
     gene            complement(31750..32190)
                     /locus_tag="KPK72_000323"
     CDS             complement(31750..32190)
                     /locus_tag="KPK72_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2487573.1"
                     /translation="MEPNSREFYKQCSFQKYCNAVLHNEACDAHRELRTHKAKEVTFS
                     DMTLDEARQLHTFDEYFKRETAETVFEKAGKKITPKLLLEAIRTLPEEKRKAILLYYF
                     EGMSDTEIAELLNTPRSTIQYRRTSSFEQLRKYLEENADEWDEW"
     gene            complement(32294..32479)
                     /locus_tag="KPK72_000324"
                     /pseudo
     CDS             complement(32294..32479)
                     /locus_tag="KPK72_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333800.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32698..32889)
                     /locus_tag="KPK72_000325"
     CDS             complement(32698..32889)
                     /locus_tag="KPK72_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487574.1"
                     /translation="MSKSEKNRDTANFQMLKEQVSEREYQNAKYEAKKKKRISYWLMI
                     IAPFIMILAIIILYYCFWR"
     gene            complement(32989..34593)
                     /locus_tag="KPK72_000326"
     CDS             complement(32989..34593)
                     /locus_tag="KPK72_000326"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012235.1,HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487575.1"
                     /translation="MKKYSFLFSYFKKAKILSLFFLMIISSVLTLLPPYLVSYILDEG
                     VVKTSIETIVYCSIFLVFIYICSFVVNYFISQNLTKTSSYFIADLKNDLFTKILKLPM
                     EFFDKQQTGYIFERIKETESLNVFFSPVFLKFVTSIVSFVGALVLVCSIRWELSLILL
                     LFLPVIYYFTNYSSIKIKKASKALLETTAQTSGKVQENIGGISAVKELKMENQRSSEI
                     SRQLKQVADKSVKRGKMMNIASEGIVGITNIASVLLIVFSGIFIIKGKMSLGNYWAVS
                     QYALLIFAPMQLLASISIMVQPGIAALTRINELLKLKTEDEICGNRQVGKIENIVFSD
                     VSFGYNEKTVIDNFNMEISRNEKVALLGKNGSGKTTIAKLLLGFYKDYSGSIRINDIE
                     IKNVSLSDLRNQVGIVAQNIVLFSGTLLDNIRFIAPQLDEHEITVALSKAGLDISEFT
                     GGLQAVISENGKNLSGGQKQKIALARMIIKDPDVMIFDEATSNLDIETSNLVKQSIHT
                     IFAEKTCIIITHDPDMASLADTIIKI"
     gene            complement(34590..35594)
                     /locus_tag="KPK72_000327"
     CDS             complement(34590..35594)
                     /locus_tag="KPK72_000327"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487576.1"
                     /translation="MQKLAVGKILQGLRKAKGITQEQLSEILGVSCAAISKWENEQMY
                     PDISFFPILARYFNVSIDCLFGFSNELTEKEYNDFKAECTELFAQKQYLNGMEKIKAL
                     CYLFPTNDRLKLDLIIAVIPYIALAQEVEIREKVVYQMITLCQNCASENVQAQKHFVL
                     GHLFMMIGQYKHFPFSFSDSEVKNFAAVDMTNSLLLRTDENAINKIETAIISLGVQLL
                     HELRNKISYLQKEDDLQGALSVTKNQIALAELLELDHSVYFMLYMNVSHLYCLLKEKE
                     NAEQAVEKFVKLLREKSIDDSVLLQVYKTGFSAKPFDLIRDTLIFAQLITMLNEEVTK
                     "
     gene            35821..36174
                     /locus_tag="KPK72_000328"
     CDS             35821..36174
                     /locus_tag="KPK72_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2487577.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPAGKDTKRRQLDALLDDMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            36435..36764
                     /gene="mobC"
                     /locus_tag="KPK72_000329"
     CDS             36435..36764
                     /gene="mobC"
                     /locus_tag="KPK72_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG2487578.1"
                     /translation="MNGRKRNIQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            36725..38068
                     /locus_tag="KPK72_000330"
     CDS             36725..38068
                     /locus_tag="KPK72_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2487579.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLTDAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNISDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYTSCRAPGQ
                     ERFTRLKTLGADYTEEAIRERIAGKRTKAAKAPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHQIEQYADLVSRIEDINAENEKTADALKSVEKRLADMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAVKTLKAAGITKLPNLA
                     TMQEEYGKLQAQKEALYADYGKLKKQVKEYDIIKQNIDSILRQGKEQERGKETER"
     gene            38140..38763
                     /locus_tag="KPK72_000331"
     CDS             38140..38763
                     /locus_tag="KPK72_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBG2487580.1"
                     /translation="MKSILLHGLGQTSSDWDKTIEVMENRMDIICPNLSEWLVDKEPC
                     YNTLYQMLENYCNQFNEPLNLCGLSLGGILAMQYGIEHTDRVNSLVLIGTQYTMPKRL
                     LRFQNMLFHIMPKSVFSQIGFQKKDFISICSSMIELDFQQSLRDIQCPVLVLCGSKDN
                     ANKTASVDMEKMIPCAKLSIIQNAGHEVNKDNPVELGNQLNTFFNLK"
     gene            complement(38932..39297)
                     /locus_tag="KPK72_000332"
     CDS             complement(38932..39297)
                     /locus_tag="KPK72_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG2487581.1"
                     /translation="MTGVKRLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQIAVLPLDKLLYAELFKTEDKKRCTECGAFFASTSNSVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(39294..39500)
                     /locus_tag="KPK72_000333"
     CDS             complement(39294..39500)
                     /locus_tag="KPK72_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG2487582.1"
                     /translation="MIRLTVEETNLLSIYSEGGKRGLMENINAALPFMDEDMRELAKC
                     TLAKIAPLTENEFAELAIFAADEV"
     gene            complement(39503..43531)
                     /locus_tag="KPK72_000334"
     CDS             complement(39503..43531)
                     /locus_tag="KPK72_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2487583.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKTGRSWNAEYFRLEMGDIL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDMNLDELTAGVRRHYENGYSNIGEFIE
                     AHDDRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYIFDGSMSMEDYELMHRMIE
                     NERSERMAETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEVFDRIGIDGKNYGEL
                     HITEYQSSIEGLAGKLTELESLDELNYLSELLKMQFDDDREKFVAAMAYGDHTRDLQD
                     IINLAQNLDCYWLYPSVQSEEDYGHYLIEELDELELPEESKKYFIYEEYGRDAAINDG
                     GRFTEQGYIYNNRNTFTEWYDGRDVPEEYRVTPQPPVQEKEQADLDASAAIPTTATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGLYESDRYADYLRTMSKFHDYSLNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDED
                     GKPVTEEKEIKIPAFRVVSVFDISQTEGKELPALTYELTGNVERYKDFFAALEKTSPF
                     AMGFEALSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAAKLI
                     DTIDGHFAEIQKAQDKEQTTAQAQPAQEATKQPEAEAAAPELSEETAPVQEKEAQPEQ
                     AAPAAPYYTINEAAAKRAKDMNSFSDYKQGSATAEYRHYVDEAVQLAERQKQRVDPMY
                     HEKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNFPTRKKEKQNAARDSNYRE
                     WQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKSQETMKAVNAYYRKHKTLD
                     GCPHLPPEELEKLKADMASSWHLEDKPFATWALSNNSAEIRRVKDRIKSLSQQKEIGF
                     VGWEFDGGKVEANTEANRLQIFFEDKPDEATREALKSNGFRWSPKAGAWQRQLTSNAY
                     YAADYVKSIQPLTGEKPTELQRAHIRQQKVAAQEQPVQEQPEQKAPQDKDTFSIYQIK
                     GGDETRDLRFEPYDRLTATGHRVDPKNYALVYSAELTPGTSLEDIYTCFNIDHPKDFK
                     GHSLSVSDVVVLHQNGQDTAHYVDSFGYKKVPEFFKEQEKALIPDNLETGETIRTPRG
                     TFYVTAMSREQMEAAGYGLHHQSDDGKYLIMGNGTRAFAVPVQPENYLKAAEQTTEQN
                     YNMIDGQINNTPTTAELEAKVKAGETISLVDLAEAVKADKERGKAAKTEKKPSIRAQL
                     RADKEKTAQKKAAKSKHHELEV"
     gene            complement(43662..45752)
                     /locus_tag="KPK72_000335"
     CDS             complement(43662..45752)
                     /locus_tag="KPK72_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2487584.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWSYDSLPILPQEWKYTVAADKEKQFKILKELMHRADVSEIVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDGAIKEGFSCLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASEKLSDRSEADRLKSACEASKAACTSLVKEKKTAAPPKLFDLTSLQREANR
                     IFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFPFIAGKD
                     FTPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESEKNILTLAGARLLMATAALHT
                     FEAVTAVFECAGQSFTARGKTILCEGWKEIDRRYRAALKSKPETDDADRDTEKTLPPF
                     TEGQTFENPTATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     TAFMEGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     DCIFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIVLADTG
                     GKYVNYRIELPKKK"
     gene            complement(45875..46348)
                     /locus_tag="KPK72_000336"
     CDS             complement(45875..46348)
                     /locus_tag="KPK72_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2487585.1"
                     /translation="MDYIIREMRSEEYCLLSDFLYEAIYIPEGIEPPPKSVIECPELQ
                     EYIVEFGNRKHDKALVAEMQGNVIGAIWVRIMNDYGHIDNDTPSLAMSVHKKYRGLGI
                     GTSLLKQLLQVEKLAGYSKISLSVQKSNYAVKMYEKVGFTIVDENNEEYIMIVNL"
     gene            complement(46371..46619)
                     /locus_tag="KPK72_000337"
     CDS             complement(46371..46619)
                     /locus_tag="KPK72_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG2487586.1"
                     /translation="MEDKKILLDNIDKIHTTEMGIDRIKRNLKIDTADVVEYCKNKVL
                     DKNCNIYKQGKNWYCEVENIKITINSYSYTIITAHIVK"
     gene            complement(46891..47619)
                     /locus_tag="KPK72_000338"
     CDS             complement(46891..47619)
                     /locus_tag="KPK72_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2487587.1"
                     /translation="MKNKLKKTMTALCAALILMGGFSVPAYAQGTATEPPAEDATNDS
                     NVVVEEKEETPPLTPKGNATLVDDFGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPEICNCTEKCEAGKVNTSCPVCVTSMTGCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFVLLGGGAAFYYFKFLKPKQNVKGNTDLEDFEFED
                     YDEDEPEADTGETPEDEETEEETL"
     gene            complement(47609..47866)
                     /locus_tag="KPK72_000339"
     CDS             complement(47609..47866)
                     /locus_tag="KPK72_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG2487588.1"
                     /translation="MAMNKIDRINKEIVKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMTSAPAEENEQEENADEE"
     gene            complement(47898..49814)
                     /locus_tag="KPK72_000340"
     CDS             complement(47898..49814)
                     /locus_tag="KPK72_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG2487589.1"
                     /translation="MTRDGAVEINETQQTAERISSREADSDFSQPDNTATERVMEHLD
                     AAHTRKASKKAVKKAQEATVLRTSTSRLQFTDEERATPELEPYIKKSEKAADKLDAAK
                     AALPKQKKLVKERTFEEATGKAKTRLHFEEQEKPIPGSKAHNNPLSRPAQEAGIFVHN
                     KIHSVEKDNSGVEGAHKSEELAERGARYGTRKLKQGYRSHKLKPYREAAKAEKAAFKA
                     NVNFQYHKALQENPQLTSNPFSRFMQKQQIKRQYAKAAKKGGAKAAAEATRKTAKKTA
                     EETRKAAAFAARHPAGILIAVAALLLFIMISAGLSSCGAMFSGSMNSVLGTSYTSEDS
                     DLVATEQSYAAMENGLQSEIDTIESTHPGYDEYRYDLDSIGHNPHELASYLTALLQSY
                     TPQSAQAELKRLFGLQYTLTLTEEIEIRTTTDEEGNEEEYEYRILNVKLTNKPIASLA
                     EELLNPQQFEMFKVYLQTQGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRPGNQELMEL
                     AKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQGVPWFQSR
                     GQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGDACKIKSYD
                     LNYQCIKGYGLMNW"
     gene            complement(49841..50770)
                     /gene="dcm"
                     /locus_tag="KPK72_000341"
     CDS             complement(49841..50770)
                     /gene="dcm"
                     /locus_tag="KPK72_000341"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBG2487590.1"
                     /translation="MKKLTHFSLFTGIGGIDLAAEAAGFSTVCQCEWAEYPNKVLERH
                     WPYVPRFQDITTVTKEAFIEKTGQETITLISGGFPCQPFSAIGPKQGFSDPRYLWPEM
                     CRVIKELRPRWVLGENVANFINMGLHKTLFDLEQAGYAVWTFVLPACAVGAWHERKRT
                     FIVGADVSHAPCFRHRLKSKCGKDKHIPERGISQEKTGRDILVGGTVGNRLFPHADPG
                     FDFALQSGMGGMADGLPTGVDGNFLWGIEPADIPRLAPDKKDRTKRLKTLGNAVVPAQ
                     VYPILRYIADIELGRCREWCVFSENGGAGLSSS"
     gene            complement(50767..53196)
                     /locus_tag="KPK72_000342"
     CDS             complement(50767..53196)
                     /locus_tag="KPK72_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2487591.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKVAQRRYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDAFNSIRTEYSDMLKTQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDVLNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFRFGEGRYFAMGKKTGAVSFLEILAPELNDRILADMLD
                     LETGVIVNLHIKSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSIPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREITNAFLITDDDIFICDPEAEYFALVQRLG
                     GQVIRLSPTGKGMDGKPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRDYLADPDPAKMPILGDLYNELLKQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELNNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNIKDLLASREVENIFENSDFVL
                     MLNQAQGDRAILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDHFPKDTELYRI
                     MTTKPEEVSSL"
     gene            complement(53144..53548)
                     /locus_tag="KPK72_000343"
     CDS             complement(53144..53548)
                     /locus_tag="KPK72_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2487592.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSAALLIGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKERPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGSRRKQA"
     gene            complement(53591..54460)
                     /locus_tag="KPK72_000344"
     CDS             complement(53591..54460)
                     /locus_tag="KPK72_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487593.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNANIFNMIQNLSENVIVPIAGLVITYVLCYELISMITEKNNMHDVDTFLFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMVQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            complement(54479..54694)
                     /locus_tag="KPK72_000345"
     CDS             complement(54479..54694)
                     /locus_tag="KPK72_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2487594.1"
                     /translation="MEFFNSAIGVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            complement(54886..55056)
                     /locus_tag="KPK72_000346"
     CDS             complement(54886..55056)
                     /locus_tag="KPK72_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487595.1"
                     /translation="MNRRIRKKKDKKITEAINGLVLIAERREQQRQAAIRQFVKRCEA
                     LYAQQRKEELTQ"
     gene            complement(55049..56839)
                     /locus_tag="KPK72_000347"
     CDS             complement(55049..56839)
                     /locus_tag="KPK72_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2487596.1"
                     /translation="MNAINWKKLILPNIPYLLFIYLFDKVGQAVQLSPGTDLSGKVLS
                     LADGFSAAFANPLPNLAPMDLLIGIVGAVLIRLIVYVKGKNAKKYRKGIEYGSARWGN
                     AEDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMKYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMIN
                     ASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLA
                     PFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKA
                     DDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKD
                     NADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLG
                     KQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHL
                     RRRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            complement(56836..57318)
                     /locus_tag="KPK72_000348"
     CDS             complement(56836..57318)
                     /locus_tag="KPK72_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2487597.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDKQKI
                     PQGKQTLKQLMKQNAGVSNIEITKDNIKAFEHTAKKYGIDFALKKDSTETPPRYFVFF
                     KGRDADALTAAFKEFSAKKLTQEQRPSIRKLLSTLKDKAAALNAQRDKVKNKDRGITR
                     "
     gene            complement(57412..57630)
                     /locus_tag="KPK72_000349"
     CDS             complement(57412..57630)
                     /locus_tag="KPK72_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG2487598.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLLVRI"
     gene            complement(57651..58631)
                     /locus_tag="KPK72_000350"
     CDS             complement(57651..58631)
                     /locus_tag="KPK72_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2487599.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPVLENPTADKPILENPIPDNPTSENPTQLNKDIQKTNLPKKEKLNKDISSTHSIPF
                     HSLNPSPLEDAAQPPERKRKETEPSAFDIYEEIIKDNIEYDILMQNNRLDKDLINEIV
                     TLMLETVCTKRKTIRIAGDDYPAELVKARFMKLDSSHIEFVLNCMSENTTKIRNIKQY
                     LRAVLFNAPSTMNSYYTSLVAHDMASGALTPKKPQYGDPDYYFCNEGESL"
     gene            58930..60372
                     /locus_tag="KPK72_000351"
                     /pseudo
     CDS             58930..60372
                     /locus_tag="KPK72_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            60401..60724
                     /locus_tag="KPK72_000352"
                     /pseudo
     CDS             60401..60724
                     /locus_tag="KPK72_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            60756..61943
                     /locus_tag="KPK72_000353"
     CDS             60756..61943
                     /locus_tag="KPK72_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG2487600.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCREFKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTNIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            62135..>62477
                     /locus_tag="KPK72_000354"
     CDS             62135..>62477
                     /locus_tag="KPK72_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG2487601.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRS"
BASE COUNT        19904 a        10645 c        12635 g        19293 t
ORIGIN      
        1 aatattttca atattctatt gcaatttatt tggaaaagtt atatatttat aacataaggt
       61 gttataaata tataactttt aaggggtgat tttatatgtc taaatcagag cttggtgtta
      121 aaacaaatct tgtttcagag ttacttatga tatcctattt ttttatagac tcattattaa
      181 aaaagtttat ttttaaatat ggaaatcttt gtattacttt tataatttta ttcgcttttt
      241 gggcatgtga cttcatgtat aataaaaaat ataatgattt agttgacgag gcgtcaaaac
      301 ttatgttatt aaaggttgat catattgcat caaaaataat gttatattct attttgctaa
      361 tagctgtttt cttttcagca aattatacaa gaggtttgga tatatcaaat ttggatatag
      421 gtgtaattct cttagctatt ttatttatac aatctttatc taaattaatt ttgtatatac
      481 attttgatag aaagggaata tgcaactagg tgataatatt gataaaaacg aaaattaaag
      541 agtatagaga atataatttt taaggagtga ttttatatgt ctaaatcaga aactggtgta
      601 agatcaaagc ttatcctgga gttgcttgta ataacttatt tttttatagc tgtattatcc
      661 actaagtata tttttaaagg tccaagcact gcttctcttc cagggttgag ttggttaata
      721 gtttttggat gttattcaat ttataataaa aaatataatg atttagttga tgaatcatct
      781 gaatttatac tatcaaaggt taataagatt gcaataaaaa taatgatata ttctatagta
      841 ataatatcta ttttctttgc aacaccttat acaagtaata taaatatatc aaacctagat
      901 acaggtataa tcctcattgc agttttattt atacagtctt tatctaaatt agtgttgtat
      961 atatactttg ataaaagggg gatatgtaac taagtggcaa tattgatgaa aacaaaaatt
     1021 aaagaataca gagaaaaact ccttatgacg caaaatgaac ttgctaaatt ggttggggta
     1081 agacgtgaga ctattgtaca cttagaaaat gggaaatata atccttcttt gaagttagct
     1141 gtggatatcg ctaaagtatt cgatactaca gtagaaaatt tgtttgaatt tattgaagaa
     1201 gaatagtgga aaatttataa acatttataa gatggataat aaaatatagt tatttaatca
     1261 ttaagtttgt taaaaatatt tatataatat aacatgtgat taaggtcaga ttataattgt
     1321 tttaagataa aatgtaataa aatttaatgg agaaatttta taggtagata aattttaata
     1381 tttataagat tatgttaagg aaacacttaa aaattagatt aaaattgtgg gtatttaaat
     1441 caactaaaag atattgacta tttagtcaat atcttttttt attaaaaagg aatatacata
     1501 taaataggtt agattaaaaa ttgggataaa tatgatttat atagcatcat ttaattaaaa
     1561 ggagctatat tagtattcat tcatacaatt ggataatata caatgtcagc cttgctttat
     1621 ggtgtttgta ataaacctat tttaatttct attgtaacta atatgcaaga atttaagata
     1681 ggagtatcta tggattatgg tgtattagta attggtatat gctatatagc attaggaata
     1741 atttttaaat tagatagaaa aagatttaca taagataaca aaggctaatt tataaattgc
     1801 tgcttagaaa accaaatcac tttattagct agagatgaat ttggagggtg tcactttggt
     1861 gttttatgta aagtttatgg aagtaaaaca ttagataatc gtgagtatga tttctataaa
     1921 tcatatatac tattggaatc caagaaaagg tattggccaa atagctaata tctttttaat
     1981 ttttttgact ctaatatagc attaactatt gcatgagtat ctttaaattt atagttaatg
     2041 tatcttaatt gtttattata aaatctagga aggctaaagc ataaaacact taaaaagaga
     2101 agatgtatat gagaaatata attaactatg atgcagactt ctaaaaatta tttagtgaaa
     2161 aggaacttag agtgtataat aaaagttcct tttcactaag gaaaacgatt gttattttct
     2221 gatcttcatt accctgatat tatttaaata taaacaacaa caaaattaac aagaagcaaa
     2281 ttcaacctaa aatttaaaat atcgggaggt acaatatgtt aataaatatg aaggaaatgc
     2341 taaaagtggc acaagaaaat caatttgctg taccagcatt taatatagga agtgggcaaa
     2401 tacttaaagc agtagtacag agtgctaacg aaaaaaatgc accagtaata cttgcaatac
     2461 acccaaatga gctaagtttt ctaggagata gctttgtagc tagttgcata gaagaagcaa
     2521 ataagagtaa agtacctatg gtaatacact tagatcatgg ggaaaataaa gaacaaatat
     2581 taagggcaat aagatgtgga tttacatcag taatgattga tggctcacat ttaccatatg
     2641 aagagaatgt agctatatct agggaaatag tagaaatagc aaaaggatta aatgtgtccg
     2701 tagaaggaga acttggaaca ataggaacaa caggaacatc aagtgaaggt ggtactgatg
     2761 aaataatata tacagattca aaattagcta aggattttgt tgaaaaaaca ggagtagaca
     2821 cactagctat tgcaattggt acagcacatg gtatatatcc aaaaggtttt aaaccagagc
     2881 ttaaactaga tttattaaaa gaaataagag aagtagtaga tataccttta gttcttcatg
     2941 gtggttcatc aaatccagat gaagaaatag cacaagcagt taaactagga gtttgtaaaa
     3001 taaatatttc gagtgatgtt aagtcggcat attataagaa gtgcagagaa ctattagaac
     3061 aaaacccaag cttatatgag ccagatacta tatatccacc atgtataaaa tctgcaagag
     3121 aagttataga gtttaaaatg aatctattca atgctataga taagttaaag tgcttttata
     3181 aaaataaaaa ttaattatga ggtaacaaat taaaaggagg ggagaattta tgataggtaa
     3241 aatactacga agcgattgta ttgtactaga taaacaatta caaacaaaaa aagatgtaat
     3301 taattatatg gcaaatcatt tgtaccatgg aaattatata aataataaag aaaagtttat
     3361 agctgatatt aataaacgcg aaaaagaatc atcaactaat atgggtaatt taatagctat
     3421 acctcatgct aagtcaaatg tagtaaatca acctgtaatg atgtatttaa gaacagatag
     3481 agagattaca gataatgatg gtcaaaaact taggtcattt tttatgatag cagttccaga
     3541 atcaggttca gatgaacatc ttaagattat atctaaacta gctacatatc taatggaaga
     3601 aaattttctt aagcatctac taagtgcaga aactccatac gaaatattaa gtatatttaa
     3661 taataaagct gcagaatcag gagattttag taataatcaa aataaaggtc ttatagttgg
     3721 gataacagga tgtccaacag gtatagctca tacttttatg gcaaaaaaag cattagagga
     3781 agcagggcaa gaacttggat atgaagttaa aatagaaact catggagcta taggtgtaga
     3841 aaataagttg actaaagaag atataaaaag agctgaatac gtaataatag cttgtgataa
     3901 agaagttcaa ctgagtagat ttataggaaa gaaagtgcta caaaagaaag tagtagatgc
     3961 taagaatgtt tcattagcta aaaatttaat tataaacgct gaaaatggaa tggctaaaga
     4021 aattaaacca ggtggaaaga ctttagaaga ttcaaaagaa atgcttcaag ggtcagagaa
     4081 aaagggtgtt tataagcact taatgagtgg tgtatctgaa atgattccat ttgtagtgat
     4141 aggtggaatt ggtatagcca tagcttttat gtttggtata tttgcatcag acccaactca
     4201 tgaaacatac aaccctatag ctggattctt tggacaactt ggaggcgatt cagcatttaa
     4261 gttatttata ccgatacttg ctggttttat agctaaatca atagcaggaa gacaaggatt
     4321 tgctcctgct atgatagctg gttttatggc agttattgga ggttcagggt ttataggtgg
     4381 tatggtagct ggttttgcaa caggatatat agctttagga gtaatgaaga taacagaaaa
     4441 tttaccagat gtattaaaag gagtaaatgc tgtattgata tgtccacttt tgattgcatt
     4501 tacgagtgga gctataatgt tctttgtagt taatacgcca gcatcatggt taaatgaagc
     4561 tttacaatct tggttacaaa gtatgtctgg aactaataaa gtattattag gtgcaatatt
     4621 agcagggatg atggcatcag atatgggagg acctattaat aaaacagctt cagcttttgg
     4681 attggctatg ttttcaagta atatatttga accatcagca gcattaatgg taggaggaat
     4741 ggtacctcca ttagcaatat cattggctat gttaatattc aaaaataagt ttactaaaga
     4801 agaaagaaag tcatctctac caactgctat aatgggggca tctttcataa cagaaggcgc
     4861 aataccgttt gcagctgcag acccattaag aatgattcca gcaaatataa ttggttcttg
     4921 tataggtggg gcaatatgta tgagtttaga tataacttta atggcaccac atgggggaat
     4981 atttgtaata ccatttgcat gtagtgcacc agttgcatat atagcttgta tattgattgg
     5041 tacagtaata acagcattga ttataggggt aacaaagcct actataagga gtgaaaatag
     5101 tgaagttgaa ttacttggtg cttaaattat aaataatagt aaaaataatt aatggaataa
     5161 caacaagtat atagaataat aataaggata tttagaatca gttaactact tctaaatatc
     5221 cttaattttt attttgaaat tatattatct attatatcaa tttagtaagg tgtatcataa
     5281 attcttttat gatacagaag tatcttagta attttacata gttagtaaat atgttaaaat
     5341 agtacttaat ataaacatag aaatatataa acataggtag ggagggctaa gtatgcttgt
     5401 gtcgaggcaa attgaatttc ttaaattgat actaaacgaa aataaatata gacctataaa
     5461 atactttaaa gataaattaa atgtatctga taaaacttta caaaaagatt tgaagatgat
     5521 tgagaaatat ttagaaactt ttaatataaa aattgatata aaaagaggct atggaatatt
     5581 aatagaagat ttagcaaaga agaacataga cttaattaat aatttaaata tacaatcgaa
     5641 ggaaaataaa tctttatcag tagagcagag aagaatggag attttaaagt atttattact
     5701 aaattcaaat aatataactt caataaatca gttagcagat aaatattatg taagtaaaac
     5761 ttctatagta aatgatttta aatatataga ggcatggata aaagaataca atttaaaact
     5821 taataaaact ttagaaggaa ctaagattat aggtaaagaa gtggatataa ggaaatcgat
     5881 tgccaaaatg atggatgatt tattagatga aaattatgaa caacatgata tatctgagtt
     5941 aacaagatta gattctgcta catttagtgc attgataaat ttatttgata ttgattctat
     6001 tatatttgta gaaactataa ttacagaatt agaatacaat ttaggatata ctataaatca
     6061 gccctactat ataaatttaa ttacccatat acttatatgt ttaaagagaa tcgaagaagg
     6121 taatcaaata gaatctcagg aagaaagaga aatttctgta gataatttgg acgagttagt
     6181 atataaaaat gtcactacac ttatagataa aatagaaaat agatacagag taaaaataac
     6241 gaaagatgaa cttatgtaca tatatatttt tttggtatca tctggattta gtagcgaaag
     6301 taatagtagt tacaatgatg aatcagatag attaagtgaa agtgaaaaag tttcatctat
     6361 aatgatagag aatatgtcac aatttttaaa tataaatctc aaaaatgatg acttactaca
     6421 aaaaagtcta gcatctcatg taaggcctat gttaaacaga ttaagatatg atatccaaat
     6481 aaaaaacccg ttgttaggag agatacaaga gcgattttca gatgtgctgg gattatgtat
     6541 gatgactata aatattatga cagagtatga taatctgaag aatattagta tagatgaagt
     6601 ttcatatctt gccacatatt ttcaagcggc aatagaaaga aatatgagta gtaaaagagt
     6661 aatagtagtt tgtcatacag gatatggaac gtctcagctc cttgcagcca gattaaaaag
     6721 agaatttcct gaatggacta tagttgatat agttcctatg catcagttga gtaagcgaaa
     6781 tttagatgat gtagactttg taatatctac tgtaaactta gatttgaaag ataaattaca
     6841 tatagttgta tcagtactat taatggaaag tgatattaat aatataaaaa atgctttact
     6901 ttctaaacct aaaaaaagta tgaacttaga tacaaactta ttaggaatgt atttagaaga
     6961 taatatatat tttaattttg atgtagctca gatggaaagt ttaataggtg tgaatttagg
     7021 gagtaaatat gaatctattt cattaggaaa agatttaaat atatatatac ataagcaaag
     7081 taaattaaat aaagggataa tgaatatcaa taatgttact agaacgattg atatttattt
     7141 agaaattagt aatcaaagtt ttttaaagaa tattttaatg gaaatatcaa atttatatag
     7201 gcaaaagaac tatataaact atataataga ttgcaagagc aaagaggata ttaaaatatt
     7261 atttaagaat aatctattgg agaaaacaaa tggaaattag agaaatatta acagaagaaa
     7321 gcattatttt agatttgaaa tcaactacaa aagaaggcat aatacaggaa ttagtagata
     7381 aattatacga tacaggaagt ataactttaa aaagtaagtt tataaaggca ttaaaacata
     7441 gagaaaaaat aggctctact agtataggtt atcaaatggc catgccacat ggaaaaacaa
     7501 aatatgtaaa gaaggcatcc atagtaatag gtaaatctaa aaaaggtgta gattggccaa
     7561 gtatagatgg cttacctgta aaggtaataa ttatgtttgc tatgccaaaa tctggaggca
     7621 atgaagatat gatattaata tctaaaatag cttacatttt aggtggtaaa aatataggtg
     7681 aaagactttt aaatgcacat acaaaaaaag aaatattaga tatattttat gaagtgtagg
     7741 tattgtataa attataaatt tgaggttttt aaaataaaat gtgtattctt ttaaatagta
     7801 tagattaatt tttagacact agagaggaga ggatatatat ggcaatatat ttagaaatgc
     7861 ctaaattaat acatgaattt ccttttcgta ctctatcaaa tgaaggagaa gtattaacaa
     7921 caccacactg gcataaggaa atagaaatat tacttataac agaaggtgta gtaaatctat
     7981 ttataaatga caagcctatg caattaaaaa aaggcgagat agtgataata aaaggaggag
     8041 atattcatta tatactgcca tcaccgggaa gtgaaagatt agtatttcaa tttgatatat
     8101 ctttttttaa tgatttaatt ttattaaatg aggaaaaaga aagcttaatt aatttattca
     8161 gtagtataaa aaaatgtagt agagattggt cagcaaaaat aagaaagtca gttttcgata
     8221 tattgataga tatctacaat gaagacctat acaaaataga aggatatagt tatgcaataa
     8281 aaggtaaaat gtataattta attcctatat tatatagaca gattcctaga gaaattgaag
     8341 aaaatacagt agaatatgaa ataaactcta aagagatatt agagagattg aacaatatat
     8401 tcaaatatgt agaaaaaaat tataaacaac ctataaaatt agaagatgtg tcttatgaag
     8461 taggatttag tgtatattat tttactaaat ttttcaaaaa aaatacagga aaaacattta
     8521 taactttttt aaatgaatat agattagaaa aagcaaagtg gatattactt aataacaata
     8581 tatccattgt agatttggtt gaagaagttg gttttggaag tacaaaaact ttttacagag
     8641 tatttaaaga aaaaattgga gtctctccat tagaatttaa aaaaagatat aatagcttgg
     8701 agaaaaaagg gagtgtctca aaatgaactt agttcatgag gcactttctt ttatatgaaa
     8761 tcaaatatat tttcagtatt tcaacaatga aaattccatt catttataaa gtctaaattt
     8821 tagttctcaa tacaacctct atttcgaaat aaatcctttt atatatataa atatacagca
     8881 atatatgaga ataagataac aataatgagg atataaaaat gtatttgcta tatatataat
     8941 tatatacata gatatctaat attgatattt aatatgaaag tattgaaaat agacaatatt
     9001 aattatagtg agtaaaaaat ataattggat ttttaaattg aattatttaa aaggtatagt
     9061 atgaaaaaaa tcttataact aaatatgaga tagaaattat aaaactttat attgtgaaag
     9121 aggtagatgc atgaaattag ggaaagattt tttatgggga ggggcaattt cagccaatca
     9181 aagtgaagga ttttatttag atggaggaaa aggtttatct cagtttgata aactcccatt
     9241 aaatgataag agattaaaag atgtatgttt agatgaggat aatattttaa atgaaaataa
     9301 tcaatactat ccaagtcata tagggattaa attttgtgaa aattacaaag aagatatagc
     9361 aatgttagca gaaataggtt ttaaatgttt tagattttct atatcttggt caagaatatt
     9421 cccaacagga gaagaaagtg aaccaaatga aaagggatta caactttatg ataatataat
     9481 aaaagaatta aaaaaatata atatagagcc attaataacg ataagccact ttgatatacc
     9541 ttattctcta gttgaaaaat attctgggtg ggctgataga agagtggtta atttatatgt
     9601 aaaatatgct gaaattttga tgaaaagata taaagatgat gtcaaatact ggattccttt
     9661 taacgaaatg aatatgataa tgcatattcc atatataggt gctgggttaa cttttatagc
     9721 tgatgaaaat caacttcaga aaaaatatca agcagcacac catcaattat tagctaatgc
     9781 aaaaactata gaaataggaa ggtatataaa taaagaattt aagtttgggt gtatgttagc
     9841 tgctggaaaa acatatccat acacatgtaa accagaagat gtatttgcaa ctttagagca
     9901 tgaaaggcat aatttgtttt tttctgatgt tcaagttagg ggtgtgtatc caagctttat
     9961 gaattattat ttaaaatcaa atcaaataaa attaataata aaagaggaag atttatcaac
    10021 acttaaggat aatactgtag attttgtatc ttttagctat tattctagtg cttgtagttc
    10081 agctagagct gaaggtttag aaaaatcaaa ggcaaacgga ccagaaacca taaaaaatcc
    10141 atatcttcca aaatcaaata gtgtatggca aaacgaccct gtagggttaa gaataactat
    10201 gaatcagtta tgtgaaaggt atcagttacc tttatttata gtagaaaatg gactgggtac
    10261 tcaagatatt gtaaatgaag atggaaaaat aattgatgat tatcgtatag attacatgaa
    10321 agaacatatt aaaaatatga ttcttggagt aaatgaagat ggaatagagc ttataggata
    10381 tcttatgtgg ggatgtatag atgtaaccag tgtaagtgaa ggaaagatga caaaacgata
    10441 tggcataatt catgttgatt ctgatgacta tggaagaggc tcatttaaaa gaactaaaaa
    10501 acaaagtgct ttttggtata aaaaagtaat agaaacagat ggagaaattt tatttaatga
    10561 tatggaaact catgcataat tcattttaag aaaggagtgt tattatgagt ttaatattta
    10621 ctactaagga ccaacttata gagcaagggt ttagtaagga tacaataaaa tccaatgaaa
    10681 cgttgtttac actagctaat ggacatttag gagttagagg gaatattgaa gagtctgatt
    10741 ttaataatga gtatgtagat aatatgggta catatgtaaa tggattttat gaagaatctc
    10801 caattacata tggagaaagc gcttatggct atgcaaaaca aaatcagact atctgtaagt
    10861 tacctaatgc tcatatagta aatttctcta ttgaaggaga ttattttgat ttatcaaaag
    10921 gtattacaag tgaacattct aggatactag atttaaatga agggattctt aaacgttcat
    10981 ttatatggga aaactctcgt ggtagaaaag ttaaagtatc tatagaaaga ttagtatcac
    11041 aagatataga agaattaatg atgatttcat atacaataac accattgaat tttaatggaa
    11101 aaataaaatt taaaaataaa atggaaaata gtatggagaa gtttaattgt gaaaaagtag
    11161 atgacccaag agtggcatct ataaataaga agcaatttaa aattaatgta aaaaaatata
    11221 acaataagta ttttatgaat ttagttacag aaagaagtag attagggtta tggtgtgggt
    11281 ttgattcata tattacttgt ggaaatatta caaatgagac ctataatgtt tcagatgaat
    11341 gtattgagcg agaaatagaa gtctatgcca aaaaagatga aaggttaaat ttagaaattg
    11401 ttgtaggcta tagtagagta tattctgatt taaatctaaa tgaagaacgt ttaaaatcat
    11461 tagaatgtct ggagtcgtta aaatgtttag aatcagaagc acatttaaaa tcattagaat
    11521 tggttttaag agaagcatgt aatattggat ttgataaagt caaaaatatt cagagaaaga
    11581 aaatgaatga gttttggaca acctctgatg tagagattga aggcgataaa actcttcaac
    11641 tggggataaa aaccaattta tttcatattt atcaatcagt aggaagagat ggaaagacta
    11701 atatatcagc aaaaggtctt acaggtgatg gctatgaggg gcattatttc tgggatacag
    11761 aaatgtatat attaccattt tttatatata ctcaaccatc cattgccaga gctttattag
    11821 attatagata ctcaatctta gatgcatcaa gagaaagagc tagagaactt ggaataaata
    11881 aaggttgctt atattcatgg agaactataa atggtaatga atgcagtgca tattatccag
    11941 caggaacagc acagtttcat ataaatgcag atatagctta tggtattaga acgtattttg
    12001 aagcaactaa tgatgaagaa tttatgatgc aaaatggctt agaaattctt atagaaacag
    12061 caagattttg ggttgaattt ggagattata tagaatcaaa aaataataaa ttctgtataa
    12121 atggagtaac aggaccagat gaatatacag caatagtaaa caataattac tacactaatt
    12181 taatggctaa gtataattta atatatgcag tacagatggt agataaattt aagcataatt
    12241 tattttcaaa tgaagtattt agaaaagtta aattacaaga atatgaagtg aaaaactgga
    12301 taaatgccgc tcaaaatatg tatttaccat atgatgaaga gaagaaaata agcaaacaag
    12361 acgattcatt tttagaaaaa tcagtatggg attttaaaaa tacaccaaaa gaaaaatatc
    12421 cattacttct tcattatcat ccattaacaa tatatagata tcaagtaaat aaacaagcag
    12481 atactgtact ttcagcgttg cttttccctg atgagtttag cttagaacaa aagaagagag
    12541 attttgaata ctatgaaaaa ataactactc atgattcatc tttgtcaagg tcaatatttg
    12601 gaatgatggc aagtgaaatt ggaaatcatg aaaaagctta taactatttt atggacacag
    12661 cattaatgga tattaataac ctacaaaaaa atacaagtga tggaatacat gctgctaata
    12721 tgggtggaac atggatgagt ttagtatatg gatttgcagg aatgaaaata aaaaatgata
    12781 aattaagttt tgaaccaagg atacctaaac actggaaaaa tattaaattt aatatattat
    12841 ttagagataa tttagtttct gtaaatatag aagaatataa atcttattat aagttaatca
    12901 aaggtagaaa tataaccata ttacatgata ataaagaaat aaaattatca aagaacataa
    12961 tagaagttaa taattgtttt aaagggagta attaatatga aagcagtctt atttgatctt
    13021 gatggagtta taacagatac tgctaaatat cattttgaag cttggcatga gatagcaaaa
    13081 tcgctagata tagaaataga tgaaactttt aatgaaaaat taaaaggcat aagcagggaa
    13141 gaatcattag ataaaatatt gttatctgct aataaagtag atagttatac taaagaggaa
    13201 aagttaaagg tgtgtgaaga aaaaaataac atttatataa agttaataaa ctcattaaca
    13261 tataaagata tattaccagg gataaatgag cttataaaag aacttaaaaa aaataatgtg
    13321 aaattgggaa tagcatctgc tagtaagaat gcacctagga tattagaatc tttgggaata
    13381 aaagatgaat ttgattgtat agtaaatcca tctttattaa aaaggggaaa accatatcct
    13441 gatattttta ttgaagggag caagattcta aatttaaaac aatatgattg tgtaggaata
    13501 gaagattctt attcaggtat taaagctata aatgctgcta atatgacatc aattggtgtg
    13561 gggaaaaaag aagtactagg aaaatgtgac catattgtag aagatactag cttattaaat
    13621 tacaatacac tattacaagc ttggagtaaa aagattaatt tggattaaaa ttatattaaa
    13681 aattatttta aaagagagga taaggtatga agaataaata tgaagatgta gctcaattga
    13741 taattgatgg acttggagga aaggataata taattgactt aactcattgt atgacacgat
    13801 taaggttcat cttaaaagat gaaaagaaag tcaatgcaga taaattaaaa tctatagata
    13861 aggtagttgg agtaaactca acttcaactc aatatcaggt cataataggt aatgaggtag
    13921 gtgttgtatt taatgcaata ataaataagg ggataaatac cacaggagat attaaaagcg
    13981 atataaaaaa taaaaataaa aaaaatagta ttttaaataa tataatagat acaataactg
    14041 gttgtatgac acctatgata ccagccctta cagcatctgg gatgataaaa gttatattat
    14101 ctctttgcac cacttttaat tggctatcat caaattcaag tacatataga gtattagata
    14161 taataggaga tgtagcattt tactttatgc ctataatact tgcaataaat gcagctaaaa
    14221 aatttaatgt taatacatca atagcagtta tagtagtagg tgtgttttta catccaaatt
    14281 tctctgcatg ggtatcaagt ggggaccata tatcatttat aggagtacca attcaaggtg
    14341 ttatatatgc agcatctgtt atccctgcac ttttaacagt atggatgatg tcatatatag
    14401 aaaaattcat tgataaatta acacctagca tgttaaaaac aattttaaat ccaactttag
    14461 tattattaat aagtgcgcca atagcattaa tagtaattgg accaatagga aatttcttag
    14521 gagagggatt agctagtata ataaatttac tacaaggaag gttaggattt ataatggttt
    14581 gtttattagc agctgcaatg ccatttatcg ttatgacagg gatgcatcat gcattgactc
    14641 ctatattcat atcagcattt gcaactacag gacaagaatc gctaatatta atatctcaag
    14701 tttgtgctaa tttagcgcaa ggtggcgcga cattagctgt agcattaaga agcaaagata
    14761 gtaagatgaa acagttagca tcagctgcat ggatatcagc tatcatgggt ataactgaac
    14821 cagctcttta tggagttaca ttgaaactta aaaaacctgc tatgttagca tctataggag
    14881 caggaatcgc agggttattt gcaggaatta ctcatgtgac tttatatgtt ccacaaaata
    14941 gtataatggc agtacttgga ttatcagggg aaaaggggat ttcaaatata gtaaatggac
    15001 ttatagttat atttatagca ttggttgctc catttattat gacatatgtt tttggagttg
    15061 aaaaaaatgt taaggaaggt atagataatg tagtagagga agtataatat taactattta
    15121 cattaataga atttttatta ttattgacta taacttgttt tgtatttata ttgatgcaaa
    15181 gaaataagtt aatattaata agaataatta ttagaaatga caaaaagcaa cataaaagat
    15241 tgatgaagta tatggaaata aaaagttgac aaaatatgtc tgatgtttta tcatattaat
    15301 tggaattagg tagtaattgt ttaagtacaa gctaagctta ttcattaata atagcagtat
    15361 cctaggaagg agtatgccca ttattatgga taagcttttt ttatgcttaa aaggaggata
    15421 atcatgataa ttaataagat attaaacaat aatgttgtaa tcacattaga tgataatgat
    15481 gaagaagtta tcgttatggg taaaggaatt ggttatcaaa aatcaaaagg caatctaata
    15541 gataaaacta aagtaaataa agtatttaga atatcaaata aagaaatatc aaacaaacta
    15601 caagaattac tcaataatat tcctatagaa cacatgaaat tatcaagtga aattatagaa
    15661 tacgcacaaa taaagttaaa caagaagcta aatgaaagta tttacatatc cttaagtgac
    15721 catacatatt ctgcaattca aagaatgaaa gaaggaataa atgtaaaaaa tgcaatcctt
    15781 tgggaaatta aaagatttta taaagaagag tttgaaattg gaatgaaagc actagatatt
    15841 attgaaaaca aaacaggtat aaaattacca gaagatgagg ctggatttat agcctttcat
    15901 atagtgaatg ctcagttaag tgaaggtcat actttggcat ctgatattac gaagttaata
    15961 caagaagtac ttagcatagt taggtatcat tttaggatag aatttcatga agaatcagtg
    16021 ttttattata gatttataat gcatttaaag ttttttgcgc aaagattgct tttagatagt
    16081 gcacatgaag gtgagacaga caaagaatta ctaagcatta ttaaatctaa atataataaa
    16141 gagtttgaat gtgtagttaa aattaaaaat ttcattaaaa aacaatataa ctacatatta
    16201 acatatgatg agcttatata tttaacaata catttagcta aagtagttaa agactcaaat
    16261 atgtaagtga aataattttt cagtagtata ttaagaatga ttgaaaggag tgatactaac
    16321 aaaattatac ttatggttgg atggtaacat tgagttggcc aaaccttcaa atttcaaaaa
    16381 attaatttaa acgaaatatg gaggagaaaa atgggaaaat acaaacaatt agcacaagag
    16441 atagtaaaca atgttggtgg aaaagaaaat attagtgggt tagtacactg tattacaaga
    16501 ttacgtttta ctttaaaaga tgaaagtata gcaaatgaca atgtattaaa aaacatggaa
    16561 ggtattgtta ctgtaatgaa aagtggagga caatatcaag tagttatagg aaatcatgtt
    16621 gaagctgtct ataaagatgt agttgaaata attggtttag atgattcaag tacaagttca
    16681 gaaactaaaa aatcaggtaa tatactagat aaaggtatag atataatatc aggaattttt
    16741 cagccagtgt taggaataat ggcagcttgt ggtatgttga aaggatttaa tgcattattt
    16801 gtagcaatgg gattatatag tgatgtatct ggtggttata tggtaataaa tgcagcggga
    16861 gatgcattat ttacattcct tcctttgttc ttaggatata cttcagctaa gaagtttgga
    16921 ttaaaaccaa tgttgggatt agcactagga gctgcagtat gttatcctgc tatacaaggt
    16981 agcagtatat cagcaggagc agatgtgatg tacactttat ttaaaggaac aatgtttgca
    17041 tcaccagtat atattgattt ctttggacta ccaattgtat caattgatta tactggaaca
    17101 gtcgtaccaa ttatatttat agtgtatttt gcttctaggt gtgaaaaatt atttaataag
    17161 tgtgttccag atttagttaa gtttttcttt gtgccaatgc ttacattatt aataacagtg
    17221 cctgttgcac ttatagttat aggtcctata gcaacatttg gttctacact tatttcacaa
    17281 attgtaattt caataagaga ttttagtcca ctattggcag gtgctatagt agggtttact
    17341 tggcaaattc ttgttatttt tggtatgcac tggggattta ttcctgtata tataaacaat
    17401 gttatgactt taggatatga taatgttatg atgccattct ttgcctgtac atttgcaagt
    17461 tcggcagttg tgttggcaat atttttcaag actaaagata aaaaactaaa agagatggta
    17521 ataccaaact ttatatctgg tatatttgga gttacagagc cagcaattta tggaatatta
    17581 ttgcctttaa agaaaccatt tataataagt tgtattgcta gtggtattgg aggcgctttc
    17641 tatggattct ttaatcttag aaaatttata actggaggaa tgggaatatt tgaacttcca
    17701 gcaatgattg aaccagatgg gggaatgggt aacttaatag tagctctttt tggtatagct
    17761 atatcaatgg tcgtagcttt tgtattaact atgattttat ataaagatga gaaagtagaa
    17821 aagtctaaaa aagtaagaaa taagggtaaa gaagaaataa aagaagtcaa gtctactaaa
    17881 ttagaaagag aaatagtaac aagtcctata aaaggtgaag tattaaaatt aagtgatata
    17941 gaggatgcag catttgcatc tggtgtacta ggtcaagggg tggcaataat acctagtgat
    18001 ggaaaagtag ttgctccagt agatggggta gttacaacct tatttcctag tctacatgct
    18061 atagggattc tttctgatac aggtgttgaa gtgcttattc atattggatt aaatactatt
    18121 caattagagg gaagaggttt tgaggcatgt ataaaacaag gtgatagaat tacaaaaggt
    18181 caaactatat taaactttga tgttgatgca ataaaagagt tggggtatag tactgtaact
    18241 ccaattgtta ttacaaattc atcacaattt ttagatgtag tagaaacaga aagtaaaaat
    18301 attgaattag aagacaatct aattacagtc ttattttagt aaagattgag gtattagcat
    18361 aatctagtgc tttggttata attttaggag gctttgacta tgagttttaa gaatgatttt
    18421 ttatggggag gagctactgc ggccaatcaa tgtgaaggtg gatacaatga agacgaaaga
    18481 ggattggcaa atgtagatgt atgtccaaca ggaaaagata gaacagcagt tattacaggg
    18541 aaactaaaaa tgtttgattt tgatgatgag cattattatc cagctaaaac tggtattgat
    18601 atgtatcata actataaaga agatataaag ttatttgctg aaatgggatt taaagtatat
    18661 agaatgagca ttgcatggag tcgaatattt ccaaaaggtg atgaagaaac accaaatgaa
    18721 aagggacttc aattttatga aaatatattt aaggagtgta aaaaatataa aatagagcct
    18781 ttagtcacta ttacacactt tgattgtcca atgcatttag ttaaaaagta tggtgcatgg
    18841 cgtaatcgaa aaatgatagg tttctatgag aaattatgca atgttatctt tagaagatat
    18901 aaaggattag tgaagtactg gctaactttc aatgaaataa atatgatttt acatgctcca
    18961 tttatgggtg ctggtatttg ctttgaagaa ggtgaagatg ttgaaaagat aaaatatcaa
    19021 gctgcccatc atgagcttgt agctagtgca attgcaacta aaatagcaca tgaagttgat
    19081 tcaaataact taattggatg tatgtttgca gctggaagtg tatatcctta tagttgtaat
    19141 cctaatgatg tatgggaagc tacaaagtta gatagagaaa attacttctt tgtggatgta
    19201 caatctaaag gtaaatatcc taactatgcg ttaaagtata tggagcaaaa aggtattaca
    19261 ccagaaatgg aacctggtga tattgaatta ttgaataaat atacagtaga ttttatttca
    19321 ttttcatatt acaatagccg ttgtgttaga accgatgaaa atgcagatga tatggctgaa
    19381 ggaaacatat ttacatctgc taaaaatcca tatctttcat acagtcaatg gggatggcca
    19441 attgacccat taggtttgag aattacacta aatcatgtat atgatagata tgaaaagcca
    19501 ctatttattg tagaaaatgg attaggtgct aaagatatag ctgatgaaaa tggttatgta
    19561 gaagatgatt acagaattga ttatataaga gaacatataa aagctatgca tgatgcagtt
    19621 tctatagatg gggtcgattt attaggttat actacatggg cacctattga tttagtaagt
    19681 gctggtacag gtgaaatgga aaagcgttat ggatttatat atgtagatag agacaatagt
    19741 ggtaatggaa gtctaagaag aatgaaaaag aaaagttttg aatggtataa gaaagtaatt
    19801 gcttcaaatg gtgaagattt atattagaca agaaatctat gttaaataag aaatttgtat
    19861 taaataagaa gagtataaaa attaataata agtataattt ttaacttttt taactataga
    19921 aaatcaatac agaattattt tacattgtat tgatgcaata gtatgattca caaataaaat
    19981 tttagaaaat aaaattgtta agaagtatgg gaagtaagtg caatttactt tgtaataaaa
    20041 gatgaaaaag gcaagcttag gttttaagct tgtctttttt attatgtata aactatgata
    20101 ctttaaaatt ctatgaaaaa tttatttaat agagaaatta aaaatttaat ggtaatcaaa
    20161 ttctttacca atgaatataa aaaatgtcta cgtgttataa tagtttaaac taataaacga
    20221 aaggtagatg aatgaatatg aaataccaac atataataga agttgacaaa gaactttgca
    20281 ttggatgtgg gctttgtaaa aatgattgtc cagtaaacaa tataattata gaaaataaaa
    20341 agtcagtaat taaaaaacaa gattgtctaa tgtgtggaca ttgtgcagca atatgtccca
    20401 caaaagctat taccttgaca ggatttgatg aacctcctat agaacttaca aataagccaa
    20461 agctagattc agatgaatta ctaatggcaa ttaaatctag aagaagtata cgtaaattta
    20521 aagacaaaga agtttcttca gaaattataa agcaaattat tgaggcaggt aggtataccc
    20581 ctagtgctaa aaattcacaa gatgtatcat atattgtttt agacaataaa aagtcaattt
    20641 atgaaaacga agcagtgaaa ttttttagaa aaataaaacc tattgcaaat atagctatta
    20701 aatattcaaa agaagttgag attgacgata actttttctt taagcatgct cctattgcaa
    20761 taatgataat tacaaaagat aagattagtg gttcacttgc agcatcaaat atggcattaa
    20821 tggcagagtc ttatggccta ggtgttttat ttagtggata tttttcagat gtagcaaata
    20881 attcgccaaa attaaaaaag cttttaggtc taaaacgcag taatcatgtt cttacaacat
    20941 tagtaattgg atacccagat gttaaatata gacgtactgc acagaaagaa gtagcaactg
    21001 ttagatactt ataagagagg gtggatttta tggaaaaaga atgtcaacaa agaatgcaag
    21061 aaataacgtc taaatttaaa gaatgtcgca aagctatctc tgctatgggt gatgagacta
    21121 gacaattgat tttaattgct ctcttagaga gtgattttaa tggtatacga gtgggagaaa
    21181 taacaaaaaa aacacatctt tctcgtcctg ctgtatctca tcatcttaag atattaaaag
    21241 aagcagagat tgttaatttg cgtagagaag gaacgaaaaa ttattattat cttgattcaa
    21301 aaaaatcaca atggaagtca ctaacagagc taataaatct tatatttgtt ggaatacaac
    21361 atattagtga aaatgatata agaaaaggtg attaaattat acttgattta atacaaaaag
    21421 attgatttag gaacaaaagt tcactttgag acactatctt ttgaactata cccatcttag
    21481 gagacagtta aaatatgtaa aactgtataa aaaagtgggt atagttcatt ttataatggt
    21541 ttaaatatta agaatatatt attctaaata gtatctttct ctgttttgag ttttattttt
    21601 atactcaata gcttcagtgg ggcaaccaca aatacatgcc atacaatgag tacagttatt
    21661 tttccaaact ggttttttgt tttttaaatt aatattattt aaaggacata gctctacaca
    21721 ttttccacag ccaatgcatt tatcagtata atgaaatcct ttggcactaa taattgtctt
    21781 ataaaaaact gtatttatta taccactttt aattgtacct cctaagccac taggagtaat
    21841 agtttcaaat tcattttcat tgcgtatatc agaaataatc ctatatattt gtttatctgc
    21901 ttctttaatc atttgttttg atgttccttt gtctgttgta gaaaataaag ctatataatt
    21961 ctctggcatt ttaatttctg ccataccttt taattgccat tccttatatt tacatagttt
    22021 ttgaatatag tttatggatt ttgctgtatc tccaccacaa gtcattacaa agtaaactct
    22081 gttgcttcct agaaattttg tttcttttat aaaatccgta accactattg gcaatctcca
    22141 tgcataagta ggacatacaa atatgaatgg tttatctgct gatattaatt catctgtttt
    22201 ttcctctttt ataagttgat ttagtgatat cagttcgtca tttaattctt gtgcaatttt
    22261 acgagccaca tatcggctat taccagttcc gctaaaatat aaaatcataa taatatctcc
    22321 ctaactattt tttattttaa ctcttaattt tttatttatt atagtaattt attgatatat
    22381 tctattcaaa tgtatgtaaa atttagtagt taagaaaatt ataacacatt agtaattttt
    22441 atacttattg gtaaagaatt tgattaccat taaatttagc aacaagacat caactggact
    22501 tgtatatgag aagggataat aatttgctta tgaaagatta taaatataaa ggttatttta
    22561 aaggtgtaga aatatttgat aatgtaaatg accttaactt aaatacttta acaacagtta
    22621 gtagatttgt gcttgttttt ttgtttacga taacttaagg aaatatgtta cctgaataat
    22681 aaatttatat aataaaagta tataaatata tatatgaatg ttaagattag ttgatagaat
    22741 ttaaataata aatccaatat tataaacata ggaggtatga aaaatatgat taatgagaat
    22801 ttaaaaagtc taagaaaaat caaccagtat acacaagaag aattagctga aaaacttaat
    22861 gtttctagac aatcaatagc aaaatgggaa agtggagttt cccaaacaaa aggatattaa
    22921 ggaataacct gtgtttacct gcttgcgaat aaaagaataa tgctattaag ttagcgatac
    22981 tgcataaatg tgctggtatc tctttttcgt tggcttgaaa caacagacta tttcattttt
    23041 gcttgattac tctaaactta tcgggatatg atttgtcaag ggcttgttgc gtaagcaatg
    23101 cttagcaccc tttacaaatt atggacggat aagtagcttt tcaaaatcag aaaaaatagt
    23161 aatcttaaga ttttgaaaca ttgtagcagg tagtggaaaa tggtataatg taaatacaga
    23221 gaaacatata aattggagtt tgtatttaac aatcaataaa ttgaaagggc ttaatcttat
    23281 gagtaaacca aagaaggtaa tttgggtaat attgggaata attatttttg tgtttagtgt
    23341 ttttttaggg cttggatatt taggtcaaat aacaggtgga aatagtttaa tccaaaggac
    23401 agaaatgaac gataaatatg ttccggaaga aataacaaaa tattatccaa tagaaaattt
    23461 aaactcaaaa gaaaatttac tttctgataa aaattatgca aactcgatac aggatgcttt
    23521 gttaagtgcc tctattgaat ttgaacaagg cgaagaatat aaagcacata ttgataagat
    23581 tataaaggag tttgaaaacg aaaactataa aagtgttttg tatatatcag aaaaaaacga
    23641 tatagagtca agtctgacat tttcaaaatt taaaattaag gaagtagatg gtaaaaagag
    23701 atatgcttat attacaagtg tacatgaggt tataaagaaa gaccgcccgt atgacaaaga
    23761 tacgatgtcg ctactaaaat cacagttagc attaagtgat agcttgcaag acttaaatat
    23821 cagtccagac aatagtcgtt ttttatatgg atttgtacat gatgaagata tttacaatac
    23881 aaaaatagaa aataaaaaac ccgatgaaat tatttatttt gaactttgtg aaaagccttt
    23941 ctatttttgg tattatgaaa attttcaaag cgataaatct ggaaaatctt taagtataga
    24001 aattgaaaga taataataga acaattccag gttgtagagg tgattagaat gaagaaacta
    24061 tattgcatga actgtggtgc tgaaataaaa ggaggaatac ctaacgaatg ttcttgtgga
    24121 gctgaatttg atttggcaga tacaaaaaac ataaaaaaaa taattacaag cattatattc
    24181 tttgtaatat taatgtctcc actgattatt cttattcact ttggcagaca atatttacaa
    24241 gcgtcaatta ttttatatgc tttactcctg attctaggta ttttttggtg tcgtatcgta
    24301 caaactattt tagttcgtct tgggttagta actatgacga acattgaaat taaataacaa
    24361 caataacatc ttcacaactc tactgagcag gaaatctgaa aaggtctcct gcttttttaa
    24421 ctggaaaaaa agattaaaaa tctgattatc cgcatgaaca cagggacatt aagcaatgtg
    24481 aatgaaaata caaaagaaat ttatcactaa tttactacga ttgaaagaac cttgatgcga
    24541 caaagaaaaa ttgcataaaa aaggcatttc atacaagata tgagatgtct ttttgttgat
    24601 gtaacatagc ataatttgtt ctttttctgc ttgattatct atcatttttt agagcatgaa
    24661 ttgtcaagag cttgttgcgt aagcaatgct tgcactcttg ataaggcatg ggataaaaaa
    24721 tattctgaca gatagcagaa aagtaatgaa ctgaaatgag gtaagtgcta taatatattt
    24781 atcaattttg gaaaacgtgg agagatatat ggaaaagata ttagaaaaca cattattact
    24841 catgatactg ccatggatat ttgtctgggg aatactatgt atcttagaaa agagaaaacg
    24901 gaaaaatgaa gacggaaata aagatgaaaa aagatatgtt atacaaaatg ataatatatt
    24961 ctttcccctt ttttcgttag gtatgttttt attttcattt tgcctttttt atacggtgtt
    25021 ttttactgaa tttgatatat cagaagtaat atcagtttct gcctttgtca tattttttgc
    25081 tattttaaca ttagatagtg taatctggag agtaattgta gatggtgata taatcaaata
    25141 ccgttcattt tatgggatta ctaggacata taattttaat gatattacca aaggagttta
    25201 tactaagaaa ggttctttaa aaatatatat tgaagggaaa agaattttta cattttatga
    25261 tagtttagaa ttttctttgt ttgaaaatca aatgagaaaa agaaaaattc ctttagaata
    25321 taaacgcaaa gaaaaataat aaggaaattt tttagaatta gtttaataat attaccactt
    25381 taaagacgga gtgatccaga cacggtggca ggaaaatctc tcagattttt ctgccatgtc
    25441 ttgcaagtgg ctggttcact tcgtctgtga gcgagccgtg gcgagcgaag attaagcccc
    25501 cgttatctaa caaggggggc agaaaaaaca agagacaaga gacatttaca aacggaaaac
    25561 gttgtattta caaggtaaaa gtgcatttta taaggtgtga tataaaaatt acctatttca
    25621 caaaggaaaa cactatctaa ttaaatatta caataattta atttttgtgg taacaatgtg
    25681 taagctttta tggaggaaag atatggattg tgaaatgact gatattattg ctattatttc
    25741 aagtatcatt gccgttgggt attcaattta ttccatagtt acgaaaggtt atattcgtca
    25801 atacaacctt aaaaaatttg agcatttttc tttaaatcag aagaaagttt gtattgcttt
    25861 caattttatt atgacactta taattggaat aagtggagtt tatatattgc tattatactt
    25921 ttcttaatgg gattgaaaac attattgtaa ttaaaaatca ctggcttaaa tattgttgtt
    25981 ctgatatgaa aaaatataga gattgcatga aaggaaaatt tgaatgttaa ttttaggaat
    26041 tattgttgtt attgtcagct tatacctgtt gtatctaaaa tatagggttg tttttactgg
    26101 cgaaaaatgc aaaggaaaaa taataggtat tgtagaccaa aacgctgggt atgttgttgg
    26161 tggagctttt gtaaaaaaac acgcctacat tataaagatt aagaataaaa aatattatac
    26221 ggcacacggt tgtatcctgt tatcactggg aaaaagaaaa atcgggaaag aaatatttgt
    26281 atttaagaat gaaaagtatg gaaaagaggt tttcaagtgt tttgattttc gtatagaaat
    26341 cgtaactctt ttgacgtgtt tattgggggt atttctgatt tatgagagct tacaataaag
    26401 gtaaataatt tcatattcat actcataagc agttggtctt aggattgact gctttttcta
    26461 tacagatttt tattaacagg caattatcaa gatacggtag ttgccttttt tgattggagg
    26521 aattttagaa tgaatgataa aaactttatt gaaacattaa gacaaaaacg tgaggaatac
    26581 ggagtaacac aaacaagaat tgctgttgcc tgtggtatca gccgtgagta ttacaaccgc
    26641 attgaaaaag gcaaacagcc attgaatgat gaattaaagg aaatcataga aaaacagatt
    26701 gagcgtttca atccacgaga gccgttattc ctgctgattg attactttcg tgtccgcttt
    26761 cctactacgg acgcattaaa gattatccgt gatgtattgc aattaaaagc cgattatatg
    26821 ctgtatgaag attatggaaa atacggatat gaaagcaaat atgtgcttgg cgacatcaat
    26881 atcatgtgtt ccatgcagga acatttagga gttctattgg aactgaaagg caaaggctgt
    26941 cggcaactgg aaagttatct gttggcacag gaacgctcat ggtatgattt tatgcttgat
    27001 tgcatgacgg ctggcggtgt gatgaaacgg cttgatttgg ctatcaatga ccgagcagga
    27061 atattagata ttccaaagtt aaaggaaaaa tatatggctg gggaatgtgt ttcctatttc
    27121 cgtaaacaga aaaattatgg cagtacagaa aaatgtggtg atgatatgcc aaagaataca
    27181 ggcgaaactt tatatcttgg ttcaacaagt agtgaattat atatgtgtgc ttatcaaaag
    27241 aactatgagc agtatgtcaa aattggcaca gaagttgaag ataccgagat taagaaccgc
    27301 tttgaaattc gcatgaaaaa tgaacgagcc tattatgccg ttgtagacct actgacttat
    27361 cgggacgctg aacgtactgc tttttctatc atcaatcatt atgtccgttt tgtagataga
    27421 gaggacgata aaccgaaaag tcaatggaaa atgaatgagg attgggcgtg gtttgtaggg
    27481 gataacagag agccgatacg cttaactaca aagccagagc cttacacctt gcaaaaggca
    27541 ttgcattggt tacaaagaca ggttgcaccg accataaaaa tgatacaggc attagacaga
    27601 gaaaatcata caacgatact gaaagatatg attgagcagg cagaactcaa agacaagcat
    27661 aaacacttat tacagttaga aaaatcaacc atagaagaac gtatagatac cgttgttcca
    27721 caagagaatg acggtatttt ttaatttctt caaaaaatct gcaacaaaac ggctacttgc
    27781 gtacagatat ggtgcgagaa aagaaaatct aacttttttt caaaattact cattaaatcg
    27841 aggtttccat tccctatatg gagtgaaaga agaaaaattt ttgaaaaaat gtcggaaaat
    27901 cgagttgagt ggacagtgtt ctatgaaaag agaaaaacta tcatcaattt gtattcaaac
    27961 agatagtaac agttgagtat tggtgtgaaa agaagaaaat cttttcattt tcaacttaac
    28021 atttggcagt ttccgttccc tatatggagc aaagaaaatc ttttattcca cagttggcag
    28081 ttacggcgtt gcatgggtag ttagggtgtg aaaagaaaat cagatttttt catcatcaac
    28141 ttagcagaat gacactttct ttccctatat ggtacaggaa aagaaaaaag ttttgaagat
    28201 tggttacgtt tctgctcttt tccaacgcgg tatataggaa agacgatttt cttctttcgt
    28261 gttctttgac aactgaataa agcagttcaa tacgtttgac agacagcgag ccgttgaaac
    28321 agatacgcca tgaccttttc cctgctaaag cgagcgaacc ttatctgtgt tcctgtaaga
    28381 aacttgtcct ttcctaccgc catgacctgt tctgtctgat aatgatactc ccgtacagcc
    28441 acagcttgag cgttcagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
    28501 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaag atttttacag
    28561 aaagggggta tgtgttattg ctgatattgt aaaaacaagg caggaaaaga caaataataa
    28621 gcgtagcatt ggcaacagag gaaaaacaaa gattgttgta aaagagcatt tttccgaaaa
    28681 gggaaagaca atggaagaac ttctgacgga tgttatgctg gaaaaagcaa agcagacaat
    28741 cgcataaaat cggtgcagtt gtaagaaata gattgaatga caaaaagtac ccgtgttata
    28801 ctttacttgc aagcaggtat tgtaaacacg ggttagttat aaaggaggat aactgacaat
    28861 gaaacaacag atttacaata ctgcacttta tcttaggtta agccgagatg atgaattaca
    28921 gggcgaaagt tccagcatta ccacacaaag aagtatgttg cgtctatatg caaaagaaca
    28981 tcatttgaat gtgattgatg aatatattga tgacggctgg tcgggaacaa attttgttga
    29041 ggtaagataa cgtaaccttt tttgcagagg gcgttatctt acctcttttt ttatgtatgt
    29101 tagatagcat agctctttac gtcggctcct atctgtctga aataggagat actttcggtg
    29161 ggcttgtcgc ctgtatcaac tctgccgaca aagctataaa agatttcgat tttacgggtg
    29221 ttcgtttcct tatccagttc atgaataaga atacggtcaa taaattcatg gacgttttcg
    29281 taagtcagtt cttcaatcgc ggtgtacttg cgtaccagta cgacaaacct tttcacgtcc
    29341 gcgctgcgct cggtggcggt gtcgatttcc tgtgacagct ccgcaatcct ccgggtcagc
    29401 gtcttttttt cttcatcata gccggaagtc agaaaagcaa actgttcatc tgaaagtttc
    29461 cctaaagcgt tgtcctcata gagcttgcgg aataagatgt tcagctcgtc aatccggctt
    29521 tgcgccttgt caagctctct ccgctgctgc gtaagcgtct tttggattgc ctttgcgctg
    29581 cactcgttgg cagtttcaac aaactcctgt tcatgttctt tcacataaga cattacgcgc
    29641 tgcaagtctg caaggacaag ttcttttagt acgcttttgc ggatataatg cgtagtacag
    29701 agcatatcat ttcttgcccg gttgcgatag ttgccgcaag tgtaggcgtg tttgcgttca
    29761 ggtgtcccgg ctccgcgtac tgcgtacatt ttatagccgc agtcagcgca aaagagaagc
    29821 cctgagaaca ggtcgatttc atctaccttt gtcgggcggt gccgggtggc aatccgcttc
    29881 tgtgcaagct caaaggtttc ttcgtcaatc agaggttcgt gcgtgttggg gaagaaatac
    29941 cttttttcct ccgggttctt cttcgtcttt ttcgacttat aagatacctt gtaggttttc
    30001 cctgttatgg tatgccctaa atactctttc cttgtcagaa tgtcatatag cgtcttatcc
    30061 ggccagttgt accatgcgtt gagctgcggg cgggggtgtc gtttgcttcc tgtcctacgg
    30121 tagcgcagtt ccccgacggt cagtatctta ttatcccgta gccagttctg gatttcacac
    30181 atacggtcgc cccgtacata cattgcaaaa atctgtttta cgacgtgtgc cgtttccggg
    30241 tcgggtatca gatgattgcg gttattaggg tcgataaggt agccgtaagg ggcttcgccg
    30301 ttgacacgct cgcctctctg cgcctttgcc tgtttgacag cccggatttt ctttgaagtg
    30361 tcgcgggcgt aaaactcgtt aaaccagttc cgcagcgggg taaattcgtt atcttctctt
    30421 gcggtatcta cgccgtcgtt aatagcgata taacgtacct catattccgg gaatacaatt
    30481 tctatcagtt ccccggtttt tagatagtta cgtcccaaac gggagaggtc tttggtaata
    30541 acggtcgcca catttccggc ttcgacttcc cgcagcatag cttgaagtcc ctcgcgctca
    30601 aaactaacgc ccgaaaatcc gtcgtcaacg aaaaaacggg tattgaaaaa atggtgttcg
    30661 tcggcgtatt tctgtaaaat cagcttttgg ttctgtatgc tttcgctttc tcctgcctgc
    30721 atatcttctt gacttaatct gcaatataaa gcggtaatct tgtctgcctg taacattttg
    30781 tcctcctttc cgacagcaga caagcatggt atttgtacta agtgcattat accatagacc
    30841 gctgcttttg tctgtgaata aataaaagaa aacgccctat ttccgggcgt ttccggcatt
    30901 gttttcaagg tcttttcgga taagtttctt tatcttttta tcaagtgtgt ccgtagcccg
    30961 ttcactggac gaagaacata caaagtaagt tgtctttcct attttatgct ccgtcgtaac
    31021 aacggcagca tgatttccat tcatatagat tagccccctt tcaatcatgg ctaatacaac
    31081 ttatgaataa cggtttgtcc gatattcaag taacttcata agtggacttt aatcggacgg
    31141 ctattagcgt aacctcatgg gacttgcacc cctccggcgg tctgccgaag ccctacccat
    31201 tgcctgcgac gcttttaacg ctcggactat ggctgtaagg aagtatcatt atgtattctg
    31261 tgcgttatcg cccacagtct gctatatctg ctttgcggcg tggtggttct cgctcgcttt
    31321 tccagtaggg aaaaggtcat ggcgcacccg ccgtatggct cggcacaaaa ggaacgtatc
    31381 cgatttgccc tatactgcgc cgccgttttc ttgcgtcgct ttctttgtca aggttcaaaa
    31441 tggctatgct gccgcattag tagcggaacg gaaaaggggg agtaaagacg ttccaccgcc
    31501 gattgcggtt attcctctgt tctgaaattg aggacagcca tcatcagttt ggcttgcagc
    31561 cgttcccgaa tgtcgtggtc tatgccaaag tagacattcc cgcgctcgtc atacagtttc
    31621 cgcatggata aacgggctat gtagccgctg aaatgctgca agaccatttt catagcgttc
    31681 gggtcgccct ttgtcgctgc cacaatgaca ggataaggaa caagggcatt ttccgggtag
    31741 ccgggttgat taccattcgt cccattcatc agcattttcc tccaaatatt ttcttaattg
    31801 ttcaaaagag cttgtccgtc tgtactgtat tgtgcttctc ggtgtattga gcaactctgc
    31861 aatttctgta tcgctcattc cctcgaaata atacagcaat atggctttgc gcttttcttc
    31921 cggcaaagta cggattgctt caagaagcag cttcggcgtg attttctttc cggctttttc
    31981 aaagacggtt tcagcagttt cccgtttgaa atattcatca aaggtatgta gctgccgcgc
    32041 ttcatctaag gtcatgtcgg aaaaggttac ttcctttgcc ttatgtgtac gaagctctct
    32101 atgagcgtcg cacgcttcat tgtgcagtac cgcattgcaa tacttctgaa aagaacattg
    32161 cttataaaac tccctgctat taggttccac attctcacct cctttcttgt tgaaagttgg
    32221 tggtgcttcc tccttttcgc ggggtaatac ggagctttga aaaaatgcca aatattttcg
    32281 ggaatttttt caaaaaattt tttaagcata caaaatgcgc ccgtccacaa aaagacgcgg
    32341 acaggcgcat aggaataata aaaagtattt agatgattta atagttcata tctaagggta
    32401 gaaatgttcc cggcggcggt cgggcttttt ttgataagtt aaagaatgta agaatttgtc
    32461 gatatggttt atagctcatg gcggctccct cctaaagccg ccatgccttt tacagggttt
    32521 agggtcgtcg tacagacaaa aagaaacggc ggccttgaac tactcaaaac cgccgtatca
    32581 gtcagtgata caggaaagca cacgaaatcc tctgcccatc agcggttttt ttcttttccc
    32641 cgctgtggag gcagatgttt aaattaattc aatttaccat aatctacaaa ctaaatacta
    32701 acgccaaaag caataataca gtataattat tgcaagtatc ataatgaatg gcgcaataat
    32761 cattaaccaa taagagatac gttttttctt tttggcttca tattttgcat tttgatattc
    32821 tctttcggaa acttgctcct tcaacatttg aaaattagca gtatcgcgat ttttttcgga
    32881 tttgctcata aaaatctcct tcctattata ataaataagc gtgtgaaaat gtatctactg
    32941 gacgacgctt ttttctttcg ctgtcgtccg gaagaataag aagtatattt agattttaat
    33001 tatagtatca gcaagagaag ccatatcagg gtcatgcgta ataataatac aggtcttttc
    33061 tgcgaaaata gtatgtattg attgctttac caaattactt gtttctatat ccaagtttga
    33121 cgtagcttca tcaaaaatca ttacgtccgg gtctttaata atcattcttg caagtgcaat
    33181 tttctgtttt tgtcctcccg acaggttttt accattttca cttatcactg cttgaagtcc
    33241 gcctgtaaat tcagaaatat ccaatcctgc tttacttaat gctactgtaa tttcatgttc
    33301 atctaactgt ggtgcaataa atctaatgtt gtctaataaa gtacccgaaa aaagaacaat
    33361 attttgtgct acaatcccaa cttgatttct caaatctgac aaagaaacat ttttgatttc
    33421 aatatcgtta atgcgaatag agccggaata atctttataa aatcctaaaa gcaattttgc
    33481 aatcgttgtt tttccacttc catttttccc taatagtgca actttttcat ttctgctaat
    33541 ttccatattg aaattatcaa taactgtttt ttcattatag ccaaacgaaa catctgaaaa
    33601 tacaatgttt tctatctttc caacctgcct atttccacat atttcatctt cggtttttaa
    33661 ttttagcaat tcgttaattc ttgttaaagc tgcaatacca ggttgtacca taatactaat
    33721 acttgctaaa agttgcatag gtgcaaaaat caataaagca tattgagata ctgcccaata
    33781 gtttcccaaa gacatttttc ctttaataat aaagataccg gaaaagacaa taagcagaac
    33841 ggaagcaata tttgtaattc ctactatacc ctcggaagca atattcatca tcttaccgcg
    33901 cttaacggat ttatctgcaa cttgtttaag ttgacgggat atttcagatg agcgttgatt
    33961 ttccattttt aattctttaa ctgctgaaat accgcctata ttttcttgta cttttcccga
    34021 tgtttgggct gttgtttcaa gcaaagcctt tgatgctttt tttatcttaa tgcttgaata
    34081 attagtgaaa taatatataa caggcaaaaa cagcagtaaa atcaaagata attcccaccg
    34141 gattgaacat acaagaacca atgcgccaac aaacgaaact atacttgtta caaattttaa
    34201 aaatacaggg gagaaaaata catttaatga ctcagtttct tttattcgtt caaaaatata
    34261 tcctgtttgt tgcttatcaa aaaattccat aggcaacttc aatattttag taaataagtc
    34321 gtttttcaaa tcggcaataa aatagctgct tgtctttgtt agattttgac ttataaaata
    34381 attgaccaca aaactgcaaa tatatataaa tactaagaat atactgcaat atacgattgt
    34441 ttctattgaa gttttaacga caccctcatc taaaatataa ctaacaagat atgggggtaa
    34501 aagtgtcaat actgatgaaa taatcatcaa aaaaaatagt gaaagtattt ttgctttttt
    34561 gaaataagaa aatagaaaag aatatttttt catttggtaa cctcctcgtt cagcattgtt
    34621 attaactgtg caaaaatcaa cgtatctctt atcaaatcaa atggcttagc tgaaaatccg
    34681 gttttatata cttgaaggag aacactatca tcaatcgact tttccctaag taacttaaca
    34741 aatttttcaa ctgcctgttc tgcattttct ttttccttta acagacaata taaatgcgaa
    34801 acattcatat agagcataaa ataaacacta tggtccaatt ccaataattc agcaagagca
    34861 atctgatttt tagtaacaga taaagctcct tgcaaatcat cttctttttg caaatatgaa
    34921 attttatttc gcagctcatg taataactgt acccctaaag atattattgc ggtttcaatc
    34981 ttatttatag cattttcatc agttcgcaat aataagctgt ttgtcatatc gacagccgca
    35041 aaatttttta cttcactatc agagaaagaa aatggaaaat gtttatactg tcctatcatc
    35101 ataaacaaat gccctaaaac aaaatgtttt tgtgcctgca cattttcaga cgcgcaattt
    35161 tgacaaagag ttatcatttg atatactact ttttctcgta tctccacttc ttgagccaat
    35221 gcaatatatg gaataacagc aattattaaa tccagtttca atctatcgtt tgtcggaaac
    35281 agatagcata atgcttttat tttttccatt ccattaagat actgtttttg tgcgaataat
    35341 tccgtacatt ccgctttaaa atcgttgtat tctttttctg ttaattcatt tgaaaatcca
    35401 aacaagcaat ctattgaaac attgaagtat cgtgcaagaa taggaaaaaa acttatatcg
    35461 ggatacattt gctcattttc ccactttgaa attgctgcac atgaaactcc taatatttca
    35521 cttaattgtt cttgtgtaat accctttgct tttctcaaac cttgcaatat ttttcctacc
    35581 gctaattttt gcataagtaa tccctccata tatattgtac cctaattgtt ttacttgtcc
    35641 catgtacaag tggttaactt gtaatatata tttttgtaac ctattgttaa tataatgtgt
    35701 tttagcaaag caatcaacac ttgaagaaca aatagcggaa atatttaagc taatggttgg
    35761 aactatgtag acatatccaa gaagaaaggg gaacagtaaa atgtaactgg gtgaataaat
    35821 atggcaaaaa gaccagtaga aaaatatgac ttcaaagcat ttggagaagc gataaaagct
    35881 gcgcggacag gacgcaagga aagccgcaaa caggtaagtg atgaaatgta tatttcccct
    35941 cgctaccttg cgaatattga gaataaagga cagcacccca gtctgcaaat cttttttgag
    36001 cttatgctcc gttacaatat atccgtagac cagtttcttt tagatacgcc cgccggaaaa
    36061 gatacaaagc ggcggcagct cgacgctctc cttgatgata tgagcgataa cggcatacgg
    36121 attgtaacag caaccgcaaa ggaaatttca gaagtcgaaa aagaggacaa ataaaaatgt
    36181 ccggcaaaat tgaataggat ttaggaagcg ttgtaatctt tgcagattgc agcgtttttc
    36241 ttttgcattg atttggcaaa acggcttttt ctccgtagag ggaaataagg acataaaaga
    36301 taaaaaactt tcttagcaag cataggaaaa gagtaccctt ttccgtattt tcgccctcct
    36361 gtcctgcggt gcgtcggttg aaaattgctt gttgggaagt gtcccaaacc ctcggaatag
    36421 aaaggagcga ttgcatgaac ggacgcaaaa gaaacataca aatcaaattc tatgtaacag
    36481 aggaagaaag ggcattgatt gagcagaaaa tgaagctcgt ccccacccgg aacatggcgg
    36541 cgtatcttcg caagattgcc attgacgggt atatcattca gacagaccat agcgacataa
    36601 aagccatgac agcggagata cagaaaatcg gggtcaacat caatcagatt gcaaagcgtg
    36661 tcaacgcgac aggcagcgtc taccaagagg acatagagga aataaagggg gtgcttgcag
    36721 agatatggcg gttacaaaga ttaagcctat taaaagcacg ttgagcaaag cccttgacta
    36781 tatccaaaac ccagataaaa cagacgggaa aatgcttgtg tcctcttttg ggtgcagcta
    36841 tgaaacggca gacattgagt ttggatttac gcttgcacaa gccatagaaa aagggaacaa
    36901 tctcgcccac catttgatac agtcctttga accgggagaa gtggactatg agaaagccca
    36961 cgaaatcgga aaacagctta ccgacgcggt aacaaaggga cagcatgaat acgtcttaac
    37021 tacccacata gacaaagggc atatccataa ccatatcatt ttttgtgcgg tcaattttgt
    37081 tgactaccat aaatacaatt ccaacaaacg cagctactac ggcatacgga atataagcga
    37141 taagctgtgt cgggaaaacg ggctgtccgt tgtcgttccg ggcaaaggca gcaagggaaa
    37201 aagctatgcg gaatatcaag cggaaaaagt cggtacaagt tggaaaggca agctgaaaat
    37261 tgccgttgat acgcttatcc cacaagtggc aagttttgaa gaattgttgc agcggttaca
    37321 ggcggcgggc tatgagataa agccgggaaa atatacctcc tgccgcgctc cgggacagga
    37381 acggtttacc cgcttaaaaa ctctcggtgc agattataca gaagaagcaa tcagagaacg
    37441 gattgcgggc aagcgtacaa aagcggcgaa agctcccaaa gagcagcgcg gtgtatcgct
    37501 acttatcgac attgaaaaca gtatcaaggc acaagagagc cggggctatg aacagtgggc
    37561 gaaaattcat aatctgaaac tggcagcaaa gactatgaat ttcttgaccg aacatcagat
    37621 tgagcagtac gcagatttag tcagccggat tgaggatata aatgcggaga atgaaaagac
    37681 agcagacgca ttaaagagcg tggaaaagcg tcttgcagat atggcggtgc tgatgaagca
    37741 cgttaccacc taccaaaaga caaaacctgt ctatgaagca taccgcaggg caaaggataa
    37801 ggacgcatac cgggcaaagc aggaaagcag cctgatacta catgaagcgg cagtaaagac
    37861 actgaaagcg gcgggcatta ccaagctccc caaccttgcc actatgcagg aagaatacgg
    37921 gaaactccaa gcgcagaaag aagctcttta cgctgattat ggcaagctga aaaaacaggt
    37981 caaggaatac gacataatca aacagaacat agacagcatt ttgcggcagg gcaaagaaca
    38041 ggaacgggga aaggaaacag aacgatagca taagccgaca tggtatgcta taataacttt
    38101 accttatgtt cgcttacaaa aaagaaagag gatagtttaa tgaaatcaat attacttcat
    38161 ggtttagggc aaacttcttc agattgggat aaaacgattg aagttatgga aaatagaatg
    38221 gatattattt gcccgaattt gtcggagtgg cttgtagata aagaaccctg ttataatacc
    38281 ctatatcaaa tgttagaaaa ttattgtaac cagttcaatg aacctttgaa tttgtgcggt
    38341 ctttctttgg gtggtattct cgctatgcaa tatggaattg aacacactga tagggtaaat
    38401 tcgttggtgc tgataggaac gcaatatact atgcctaaac ggttattaag atttcaaaat
    38461 atgttgtttc acataatgcc caagtctgta ttttcacaaa ttggtttcca aaaaaaggat
    38521 tttatcagta tatgtagttc tatgatagaa ttagattttc aacaaagttt gagagatatt
    38581 caatgtccag tgttggtttt gtgcgggagt aaagataatg caaataaaac tgcttctgtg
    38641 gatatggaga aaatgatacc ttgtgcaaaa ttatctatta ttcaaaatgc aggacatgaa
    38701 gtaaacaaag acaaccctgt tgaattagga aaccaattaa atacattttt caatctcaaa
    38761 taaaaagtgg gcgcagacag ccaacaaaag actgtccgcg ccctgtttta tgtaggtgcg
    38821 tttttgtatc tgttacgcaa tagaacgtca tttttaggga ataaggtata aaacccttgc
    38881 cgcgtcattt tcaagcactg aaagacccgt aaatcaaggc ttattttgcc cctactgcgt
    38941 aacaagggcg cgtcgtttcc tcatacgctc cgctgcctgt ctgcgggtaa tacgcttccg
    39001 gcagtccggg cagtatttga cgctgttaga ggtggacgca aaaaaagctc cgcactcggt
    39061 acaacgcttt ttatcctcgg tcttgaaaag ctctgcatac agcagcttat caagaggaag
    39121 tacggcaatc tgaaaccatt tgcatagaag cgaataggaa ataagctgcg ggcagacaca
    39181 ttcctccccg tcgtccagta aaatacagtg tccgttatcg cagttgcagc actccttttt
    39241 tacaaggctg ttgactttcc ggctttgggg cggcgtaagc cgcttaaccc cggtcatact
    39301 tcatcagcgg cgaaaatggc aagctccgca aactcatttt ctgtcagagg tgcgattttc
    39361 gcaagggtgc acttcgccag ttcccgcata tcctcgtcca tgaacggcag cgcagcgttg
    39421 atgttctcca taagcccgcg cttgccgccc tcactgtaaa tgctcaacag gtttgtttct
    39481 tcaacggtca gtctaatcat gctcatacct ccaattcgtg atgttttgat tttgccgctt
    39541 ttttctgtgc ggtcttttct ttgtctgctc taagctgcgc ccggatagag ggcttctttt
    39601 ccgtttttgc tgccttgccg cgttctttgt cagctttgac agcttccgca aggtcaacaa
    39661 gggaaatagt ttctcctgcc tttacctttg cttccagttc tgcggtggtc ggggtgttgt
    39721 ttatctgccc gtcaatcata ttgtagtttt gctcggtggt ctgctcggca gctttcagat
    39781 aattttccgg ctgaacggga acagcaaaag cccttgtgcc gttccccata atcagatatt
    39841 ttccgtcgtc ggactggtgg tgcaatccat atccggcagc ttccatttgc tcgcggctca
    39901 tggcggttac atagaaagtc cccctcggtg ttctgattgt ttcgcccgtt tccaagttgt
    39961 cgggaatgag ggctttttcc tgctccttga aaaactccgg cactttctta taaccaaagc
    40021 tgtctacata gtgggcggtg tcctgcccgt tctgatgaag caccaccacg tcggaaacgg
    40081 aaaggctgtg tcccttaaaa tctttcgggt ggtcgatatt aaagcaggtg taaatatctt
    40141 caagggaagt ccccggcgta agctctgcgg aatagacaag cgcatagttt ttcgggtcta
    40201 cccggtgtcc tgtggcagtc agacggtcgt aaggttcaaa gcgcaaatct cttgtttcgt
    40261 ccccaccttt tatctgataa atggaaaagg tgtctttgtc ctgtggggct ttctgttccg
    40321 gctgctcctg tacgggttgt tcctgtgcag ctaccttttg ctgccggata tgcgcccgct
    40381 gtaactcggt aggtttttct cctgtaaggg gctgaataga tttcacataa tcagccgcat
    40441 agtaggcgtt actggtaagc tgccgctgcc atgctccggc tttgggcgac caacgaaaac
    40501 cgttactttt taaggcttcc cgcgtggctt cgtccggctt atcctcaaag aagatttgca
    40561 gtcggttcgc ttcggtgttt gcttccacct tgccgccgtc aaattcccaa ccgacaaaac
    40621 cgatttcttt ttgctgtgaa agggatttga tacggtcttt cacgcgccgg atttctgcgc
    40681 tgttgttgga taacgcccaa gtcgcaaagg gcttatcctc caaatgccaa ctgctcgcca
    40741 tgtctgcttt cagtttttcc agttcctccg gcggtaaatg cgggcagccg tcaagggtct
    40801 tatgcttacg gtaataggcg tttacggctt tcatggtttc ctgtgatttt tcaaggcttt
    40861 ccagtttctt ttctaacttc tgtactgcct gcgggtcgtc tgcgctgata ccgcccatgc
    40921 cggtactgcg gattttatca agaagcccct gtatgtcctg ccactcccgg taattgctgt
    40981 cgcgggctgc gttctgcttt tctttcttcc ttgtggggaa gttagagccg cctgcaatga
    41041 ggatagaggg aacacgcgcg tcaatctcat agcctttgtt catgttcgcc gccagtttcc
    41101 gggcgtaggt gtcaagcagg ctgtcgattt tctcatggta catcgggtct acccgctgtt
    41161 tctgcctttc tgcaagctgt acggcttcat ctacatagtg tctgtattca gccgtcgcgc
    41221 tgccttgctt ataatcggaa aagctgttca tatcctttgc acgtttggcg gcagcttcat
    41281 tgatagtgta ataaggggcg gcgggtgcag cctgttccgg ctgtgcttct ttttcctgta
    41341 ccggggctgt ttcttcggaa agctccggcg cggctgcttc tgcttcgggc tgtttggtag
    41401 cttcctgtgc gggctgcgcc tgtgcggtgg tctgctcctt atcctgtgcc ttttggattt
    41461 ctgcaaaatg tccgtctatg gtatcaatca gctttgcggc ggtactgcgg attgtttcaa
    41521 gagagccttt cagttctgca agttcttttc cgctgctcca cccggcgata tacccaaaag
    41581 aatagtccga cgtatcaagt ccgtaatgtt ggcagacagc ataggcgacg ctttccgcct
    41641 gtacctcgcg ggtgcggcgg tcggggcgtt ccggcatggc aagggcagta tcatgcagcg
    41701 tggcgtgggc gatttcatgg attgcggtct tgatgttctg caattcgtcc ataccctcgt
    41761 tgatgatgat acgcttttcc tcataattgc agcgtccttt tacgccgccg cttagtgctt
    41821 caaatcccat agcaaagggg gaagtctttt caagagcagc gaaaaaatcc ttgtatcgtt
    41881 ccacgttgcc cgtcagctca taggtaaggg caggaagctc ctttccctcg gtctgtgaaa
    41941 tatcaaagac agatactaca cgaaaagcgg gtatcttgat ttccttttcc tcggtaacag
    42001 gtttcccgtc ctcatcgaaa acaggctttt gggtgtccgg gtcgattttt tctacctgtt
    42061 tcttgacggt aaacggggac ggcgcaagta tcttgatagc tttttctccg ggctttacat
    42121 ggcggtcaaa ttccttttcc cattgtttat agcctttcac aagattgccg ccctgcatgg
    42181 cgataaggat tgtattgtta aggctgtaat catggaattt tgacatagta cgcagatagt
    42241 cggcgtaacg gtcgctttca tacagtccta aaatcccctg ttccagtcgg tcggtaatct
    42301 ctttcatttt atcggcgggc ttttcagaag ataggataat cgggagaacc gggggctgct
    42361 ctgtggcggt ggtcggtata gctgcggaag cgtcaaggtc tgcctgttcc ttttcctgta
    42421 ctggcggctg cggcgttacc cggtattcct ccggcacgtc gcgcccgtcg taccattctg
    42481 taaaggtgtt gcggttattg tagatatagc cctgctcggt aaatctgccc ccgtcattga
    42541 tagcagcgtc cctcccgtat tcctcataga tgaaatattt ttttgattct tcgggcagct
    42601 ccaactcgtc cagttcttca atcagataat gcccgtaatc ttcctcactc tgtacggacg
    42661 gataaagcca gtaacaatca aggttttgtg caaggttgat aatgtcctgc aagtcccttg
    42721 tatggtcgcc gtatgccata gctgcgacaa atttttccct gtcgtcgtca aactgcattt
    42781 tcaaaagttc gctcaaataa ttcagttcgt caaggctttc aagctcggtc aattttcctg
    42841 ccagtccctc aatagaggac tggtattctg tgatatgtag ctccccgtag tttttgccgt
    42901 ctatcccgat acggtcaaag acttctttca aatgctcggc agtcgtgggg aatgataccc
    42961 attcgcccgc aggtctgccc tcggtgtact ttccaagatt agaaagatac ccgcttaaaa
    43021 tcgtttctgc catgcgttca ctcctttcgt tttcaatcat gcggtgcata agttcgtagt
    43081 cctccatgct catgctcccg tcaaaaatat aggggtcggg ttcttcgccg tcccggtagg
    43141 ctttttcaga gaaaggaagc cccgcagcgt gggcggcggc tcttgcttcc tcgataagct
    43201 ccggcggcag cctgtcgtcg tgggcttcga taaattcccc gatattgcta tatccgtttt
    43261 cgtagtggcg gcgcaccccg gcggtcagct cgtcaaggtt catatcctcg ccaagatagc
    43321 cggaataata gccgtttacc acaacggcgg cagggtcagc ctgtttgatt tcttccatgc
    43381 ggcttctgtc ctccgggtag agaatatcgc ccatttcaag gcggaaatat tcggcgttcc
    43441 aagaacgtcc cgttttccaa aacgccaccc aagcgatacc gtcccttaat tcttcttgat
    43501 agtcctttac ggtgtcccgt aaacttgcca tgtgtaacct cctttcttcg gtttcagagg
    43561 gtttgggaaa cttcccaaca agcaaaaatc cccgccgttc tcggtagaga aagcagggat
    43621 tttacggaaa agggtactct tttcctatgc ttgctattca gttatttttt cttcggcagt
    43681 tcaatgcgat agttgacata tttcccgccc gtatcagcca atacgatagt tccgtcgtaa
    43741 gttttgcctg tctttgggga atagagcttt ttcacattta ccttgccgga ttttaagagt
    43801 gcagcggcaa tctttggggt aaaggttact tttcgttctt caaagaaacg gtcatttttc
    43861 cacatggtaa agatacagtc cttgttgctg caatagtagt ttttctttcc ctcatagaca
    43921 ggagaaccgc agcggggaca tttcccaagc tccggctttt cctctttgaa acgccccttg
    43981 tcggcttcgg aaagaaacgg ataggtcttt accagttccc gcgccatatc ctcgataccc
    44041 tccataaatg cggtagggtc tgccttgcct ttggcaatct gcgtcagatt gttttcccat
    44101 tctgcggtaa gctgcgggct tgtcaaggtg tccggcagga cgcacacaag gttgataccg
    44161 tcctttgtgg gaagtagctg cttgcctttt cgctccacaa agccgccctt taccagtttt
    44221 tcaatgacgg cggcgcgggt ggcgggagtt ccaagccctt tgcgttctgc gtccgggtct
    44281 gtgtcctcgc ttcctgcgcg ctccatagca gagagcagcg acgcttcgtt gtgaggtttt
    44341 ggcggtgtgg tgtcatgctc cgttaccgtt gccgtcggat tttcaaaggt ctgtccctcg
    44401 gtaaatggcg gcagggtctt ttcggtgtcc ctgtctgcgt cgtccgtttc gggcttgctc
    44461 tttaaggctg ccctgtatct tcggtcaatc tctttccaac cctcacaaag aatggtcttg
    44521 ccccttgcgg taaaggactg tccggcacac tcaaaaactg ctgtaaccgc ttcaaaggta
    44581 tgcagtgcag cggtcgccat aagtagacgc gccccggcaa gggtaaggat atttttttcg
    44641 ctttccggca gcgcggcaag gtcggtcttt gcaagctcca tagtcgggat aatagcatgg
    44701 tggtctgata cctttttact gttcaacacc tttccaagct ccggcgtgaa gtcttttccc
    44761 gctataaaag gaaatttccc acaaagcagc ttgataatgc ccgccgctgt gtcgcccatg
    44821 tcgtcagtca gatagctgct gtctgttctc ggataagtaa gcaaacgctt ttcatacagg
    44881 gcttgtgcaa ggtcaagggt ctgttttgct gtatatccaa aaatacgatt tgcttccctt
    44941 tgtaaagaag taaggtcaaa gagtttcggc ggggctgcgg tttttttctc ttttacaagg
    45001 gaagtgcaag ctgcttttga cgcttcacaa gcagatttca atctgtcagc ttcggaacgg
    45061 tcagacagtt tttcgcttgc agcttccgcg ccagataagg caaggcgcac atggtagtat
    45121 ttttctttct tgaaagtggt aatcgctgcg tccctgtcaa caagcatttt taaggtcggg
    45181 gtctgtacgc gtcccacgtt caatgtgtgg ttgtaaagga cagagaaaag gcgggtggcg
    45241 ttgataccga taagccagtc tgcctttgcc ctgcacaatg cagaagcaaa gagcgcgtcg
    45301 tattcctcgc cgttcttcag acaggaaaag ccctctttga ttgcgccgtc ctccatagag
    45361 gaaatccaaa ggcggcgcat aggtttcttg cagcccgcca tttcatagac aaagcggaaa
    45421 atgagttctc cctcacgccc tgcgtcgcag gcattgacga tttcggaaac gtctgcccgg
    45481 tgcataagct cctttaagat tttgaattgc ttttccttgt cggcagcaac ggtgtatttc
    45541 cattcctgcg gcaaaatcgg taagctgtca tagctccact ttttatactg ctccccgtaa
    45601 gcggcagctt ccgcaagccc taccaaatgt cccacgcacc aagaaacgat atagccgctt
    45661 cctgtgagaa atccgtcttt cttttccttt gccccaagta cggcagcgat tgtctgcgcc
    45721 acactgggct tttctgcgat aactaaaatc aaaaataaat cctcctttcg ggtaaaagaa
    45781 aagagcaacc gatttttcga ttgctcctac aagtgattgt ataaaattgt gcgatatgaa
    45841 atagtaaaat aatttgacaa actgtagctt tcggttatag atttacaatc ataatatatt
    45901 cctcattatt ttcatcaaca atagtaaatc ccactttctc gtacatcttg actgcataat
    45961 tacttttctg aacggataag gatatttttg aatatccagc caatttttct acctgtaata
    46021 gttgtttcaa taacgaagtt ccaatgccta atcctctgta ttttttgtgg acagacatag
    46081 caagcgatgg agtatcatta tcaatgtgtc cataatcatt cataattctt acccaaatag
    46141 ctcctattac atttccctgc atttctgcaa ctaaggcttt atcgtgtttt cgatttccaa
    46201 actcaacgat atattcttgt aattctggac attctattac tgatttaggt ggcggttcaa
    46261 ttccttctgg aatataaatc gcttcgtaca aaaaatcact taataaacag tattcttcac
    46321 ttctcatttc tcgtattata tagtccataa tttcactcca aagtccgatt ttacttaaca
    46381 atatgtgctg tgataattgt ataactatat gaattgatag taattttgat attttcaact
    46441 tcgcaatacc aatttttacc ctgtttgtat atattgcaat ttttatctaa caccttattt
    46501 ttgcaatact caacaacatc tgctgtatct atttttaggt ttcttttaat tctatcaatt
    46561 cccatttcgg ttgtatgaat tttatcaata ttatcaagca atatcttttt atcttccatt
    46621 ctttcttttc ctcaacttca aatttgttta actctgtcat ttcctatggt tggaacaatt
    46681 atatcatatt tcatatcgca agtatatctt tttatcgttc tgtcggttgt ttgttttcaa
    46741 tctttctgat acagtaccca acacaagggg actgcccctt ataagggcag gaagcgcaag
    46801 ccgggggata aggaacaggc ggcgcattat cacgccgccc gttcggtttc tgtatcatca
    46861 tcttttcaaa gggattgtcg gtaaacaggg tcataaggtt tcttcctccg tttcttcatc
    46921 ttcgggagtt tccccggtat ctgcttccgg ctcgtcctcg tcgtagtcct caaattcaaa
    46981 atcttcaagg tcggtatttc cctttacatt ctgcttcggc tttagaaact taaagtagta
    47041 gaacgctgcg ccgccaccaa gcagtacaaa caagactaag gcaagcacca caccgggatt
    47101 gccgcctttt tctttcggtt cttccggctg ctccgttggt gttggggtgg gttctttgcc
    47161 tgtgcagcct gtcatactgg taacacatac cgggcaggac gtgtttacct ttccggcttc
    47221 gcatttttcc gtacagttac agatttccgg ctttttggca gcttcgccgt tttcggtcag
    47281 tgccataagg tcggcttcgt ccacttgatt taggaaatgt acggtctgtt cgccctcggc
    47341 agcgcggtca atgaggatat agaaatagtt gccgcttttg gtggtaacag tgataagctg
    47401 cttattgccg ccgaaatcgt caaccagtgt tgcgtttccc tttggggtaa ggggcggcgt
    47461 ttcctctttt tcctccacaa ctacgttgct gtcgttagtc gcgtcctctg cgggcggctc
    47521 tgtggctgtg ccctgtgcat aggcagggac agaaaaaccg cccataagga taagggcggc
    47581 acaaagggca gtcatggtct ttttcagttt attcttcatc tgcattttcc tcctgttcgt
    47641 tttcttctgc gggtgcggaa gtcatgccgg gaataccgcc gccggacagc atagcgttaa
    47701 gttcctgcgg agtcagacgc atagctcgta caagctgtac gatttcaagg ttttccgctt
    47761 cggttttctg tgcttccagt ccttttaact tgttctgata ctctgtgatt ttctcgcggg
    47821 tctttacgat ttccttattg atacggtcga ttttgttcat tgccataaat aaattctcct
    47881 ttcttttcgg ggctgcttta ccagttcatc agcccgtagc ctttgataca ttgatagttc
    47941 aggtcatagc tctttatctt gcaggcgtcg ccggaattgc cctcaacggt atagacgcgg
    48001 ctgccgtccc tgccaagcac aagccccaca tggtcggcaa cgccgtctaa atcccaatcg
    48061 aaaaagatag cgtcacccgg tgcgatattc tcatagccgc gtgcgcccca ttgtcccctt
    48121 gactggaacc aagggacacc ctgccattcg cagcccgcaa agcgcggttc gctttttccg
    48181 gcttgattgt agcaccatga tacaaagcag gcgcaccatt ccacgcggct gttaaagccg
    48241 taccaagacc agtaagggta accgcccaca tttccgactt gctgctttgc aagctccata
    48301 agctcttgat ttccggggcg cgtaccgttt acaaactcca cgccgcttaa atcctcggac
    48361 gggctgccgt cgggagaacc gccgccaaaa atcaacggtt tgttgccctg tgtctgtaag
    48421 tacaccttga acatttcaaa ctgctgtggg ttcagcagtt cttccgcaag ggaagcaatc
    48481 ggcttgtttg tcagcttcac gttcaagata cggtattcgt attcttcctc gtttccctcc
    48541 tcatcagtgg tggtgcgtat ctcgatttct tccgtcagcg tcaaggtgta ctgtaatcca
    48601 aagagccgct ttagctctgc ctgtgcgctc tgcggtgtgt agctctgcaa aagggcggtc
    48661 agataagacg caagctcatg tgggttatgc ccgatactgt caaggtcata gcggtattca
    48721 tcatagccgg ggtgggtact ttcgatagtg tcaatctcac tttgcagccc gttttccatt
    48781 gcggcatagc tctgttccgt tgccacaagg tcgctgtcct cggacgtgta ggaagtccct
    48841 aacacgctgt tcatgctgcc ggaaaacata gcaccgcaag aggacagccc tgcggaaatc
    48901 ataataaaga gaagcagcgc ggcaactgca atcaaaatgc ccgccgggtg tcttgccgca
    48961 aatgcggcgg ctttcctcgt ttcctcggcg gttttcttcg ctgtctttct tgtggcttcc
    49021 gctgcggctt ttgcccctcc tttctttgcc gcctttgcat actggcgttt tatctgctgt
    49081 ttctgcatga aacgggaaaa aggattgctt gtaagctgcg ggttttcctg caaggctttg
    49141 tgatactgga agttgacgtt tgccttaaac gctgcctttt ctgcctttgc cgcttcccgg
    49201 tagggtttca gcttatggct gcggtagccc tgtttgagtt ttcttgtccc gtaccttgcg
    49261 ccgcgctcgg caagttcctc cgatttatgc gcaccctcca cgccggaatt gtctttctcg
    49321 acgctgtgga ttttgttgtg aacaaaaatc cccgcttcct gcgcggggcg ggataacggg
    49381 ttattgtggg ctttgctgcc gggtatgggc ttttcctgtt cctcaaagtg caggcgggtc
    49441 tttgcctttc cggtggcttc ctcaaaggta cgctctttta ccagtttctt ttgcttcggg
    49501 agagctgctt ttgccgcgtc cagtttgtcg gcggcttttt ctgatttttt gatgtaaggt
    49561 tccagttccg gtgttgcccg ttcctcgtcg gtaaactgta agcgggaagt ggaagtacgc
    49621 aaaacggtgg cttcctgcgc tttcttgact gcctttttgc ttgcctttcg ggtatgtgct
    49681 gcgtccagat gttccatgac gcgttccgtt gcggtattgt ccggctgtga aaagtcgctg
    49741 tctgcttccc ggctgctgat acgctcggca gtctgctgtg tttcgttgat ttccaccgca
    49801 ccgtcgcggg tcattttttg tgtgatttta tcgcgtggtt ttaactgctt gatagtcctg
    49861 cacctccatt ctctgaaaat acgcaccatt ccctgcagcg tccaagctct atatctgcga
    49921 tatacctaag tatcggatat acctgtgccg ggacaactgc atttccaagc gttttcaatc
    49981 gttttgtcct gtcttttttg tccggggcaa ggcggggaat atctgccggt tctatgcccc
    50041 ataggaaatt tccgtccacc cctgtgggaa gcccatcagc cattccaccc attccggatt
    50101 gaagcgcaaa gtcaaatccg gggtcggcgt gaggaaataa acggtttccg acagtcccgc
    50161 cgaccaaaat gtcccgtcca gttttctcct gcgaaatgcc ccgttcgggg atatgcttat
    50221 cttttccaca tttgcttttg agcctgtgtc ggaagcaagg ggcgtgggaa acatcagccc
    50281 ccacgataaa agttcgcttg cgttcatgcc acgcgccgac ggcgcaagca ggaagtacga
    50341 atgtccaaac tgcatatccc gcctgttcca agtcaaagag cgttttgtgg agccccatat
    50401 tgatgaagtt agcaacattt tcgccaagca cccagcgggg gcgcagttct ttaatgacgc
    50461 ggcacatttc cggccagaga taacgggggt cggaaaatcc ctgtttcggg ccgattgcgg
    50521 agaacggctg gcaggggaag cccccggaga taagcgtgat tgtttcctgt cctgtttttt
    50581 caataaaagc ctcctttgtt acggttgtaa tatcttgaaa gcgcggcaca tacggccagt
    50641 gtctctcaag cactttgttg gggtattctg cccattcgca ctggcagacg gtagaaaacc
    50701 ccgccgcttc cgcagcaagg tcaattccgc caatcccggt aaacaggcta aaatgcgtta
    50761 attttttcat aggctgctca cctcctccgg cttcgtcgtc ataatgcggt aaagctcggt
    50821 gtctttcggg aaatggtcta caaagggcag caccacattt ccataaaaga taagtccctc
    50881 gcctgcttcg gtgtgggtta catacttcat ctgctgtggg ctgatattga gctgctttgc
    50941 aaggatagcc cggtcgccct gcgcttgatt gagcatgagg acaaaatcag agttttcaaa
    51001 gatattttcc acctcgcggc ttgccaaaag gtctttgata ttctgtgtaa tggctgtcgg
    51061 tatgccgccc catttacgga aacgcttcca aatctcaacg gaataggctg cggtctgttc
    51121 ctcttttaag agcaagtgaa attcgtccat atagtagcgg gtggactttt tctctgcccg
    51181 gttaatggta acgcggttcc atatctggtc ttgcacaatg agcataccta actttttgag
    51241 ctgtttccca agctgcttaa tatcaaaaca gacaagacgg ttattcagtt ccacatttgt
    51301 tctgtggttg aatacgttaa ggctgccgga aacatacagc tccaatgccg ccgcaatgcg
    51361 ggcagcttcc ggttccggct gttttaacag ctcgttgtaa aggtcgccca agataggcat
    51421 tttcgccggg tcggggtcgg caaggtagtc ccggtacaca ttccttacgg cgcggtcaat
    51481 gacggtctta tcaacgggct gcaagccctc cttaccacca atgacaagct cgcagaggga
    51541 aaggataaaa tcacttttca gtgcaagggg gctgtcgtcc tcgctgtaat tgaggttcat
    51601 atccataggg ttgacatact ggggcttgcc atccatgcct tttcctgtcg gggataagcg
    51661 tatcacttgc ccgcctaacc tctgcacaag ggcaaaatac tcggcttccg ggtcgcagat
    51721 aaaaatatcg tcgtcggtaa tgagaaaagc gtttgtgatt tcccgctttg ccgcaaagga
    51781 ttttccactg cccggtgttc ccaagattaa gccgttggga tttttaagct gcttgcggtc
    51841 gcagagtatc atgttgttgc ttaaagcgtt cagcccgtaa tacagggcag ccccggtctg
    51901 aaatagctcc tgtgtgatga acgggataaa aatagcggtg cttgatgtgg taaggccccg
    51961 ttgtatcggg ataaggttct cccccaaagg gatagaggac atcagccccg cttcctgctg
    52021 atagtcaaga cgggtcaagg cgcagttgta tttctgtgca atgcccgccg ccgcaaaaat
    52081 gtcattttcc agtttccgct tcgtgtctgc catgtttacc acaaggaatg tgagaagaaa
    52141 cattcgctcg ttgcggctct gtaaatcctg caacaaattc tttgcttcat ttccaaaagt
    52201 ggcaaggtcg gacgggatta tatccatgtc ataaccgctt ctaactgcct ttttctgttc
    52261 ctcaatcttc atcttgtcaa ggtcagtgat ttttcgctta atggtcttga ttgcttccga
    52321 ctggtcgata cttttaatgt gaagatttac aataacgccc gtttccaaat caagcatatc
    52381 cgctaagata cggtcgttca gctccggggc aaggatttca aggaacgata ccgcacccgt
    52441 ctttttcccc atagcaaaat aacgtccctc tccgaacctg aaagaggacg gggcgataaa
    52501 gtcctttgtg gtaagccctg tcggggtcag ccagtcataa gagaatgaga acggctcgcc
    52561 cgccgggtgg aataccccat gcagcacctt taagcgttca tagccggata aggggtgcgc
    52621 cgatacgcca agcaccttga agttattgag tacgtcggtt tcaatacggg aaaggcgcgc
    52681 ccttgctgcc gccggattgt ccgcttcaat ggaaaatgtg atgtacttgt gctttacaag
    52741 cccgttgttc cctttggcaa gctgcgtttt cagcatatcg gaatactcgg tgcggataga
    52801 gttaaaagcg tcattttgcg gcgggatatg gattgcccgc gctgcctgtt cctgctgtga
    52861 gccttgattg atgaaagaaa gctgcacgtt cacgcttgcg tcaaagtaat tgaggaaatc
    52921 gcaccagttt tcaaaaatgg cggtcttatc gtctgcctgt gcaagctgat agttgatgtc
    52981 ctcaaaggca atgctctttg aatacctgcg ctgtgccact ttgcagatac cgtccgggta
    53041 catggcaaga tagggaatgg tctgctgtgc ggtgtgggct ttgccgtccc cctttgcctg
    53101 ccggataacc gccgctatct gtttcttttc cgcgcgggtc agtttacgcc tgtttcctgc
    53161 ggcttcccgc gtcttttttg tctgtccttt tgacaatcgc tgatacctcc ttttctaatg
    53221 cccgctgttt ttccaaaagt gcatacagat tttccgtctg atacggtcgc tctttggggc
    53281 gggtaaattt tgtctgaatg atgttcttta ccaccacttc aaggggctgt ccatgctttt
    53341 catacatggc aaccaagaag cagggaagca tgacgacaat cattgccatt gccgcaaggc
    53401 ttgtccctgc gctgtcttta agcaaaaaga aaagcggcaa tccgataaga agcgccgctg
    53461 aaaaacaaag aatttgccgc tttgtcagat tgaaagcgac ttttgttttg actttggata
    53521 agtctttggg tacaggtaca tacgccaaga aaaaacctcc tttctgcggg actttcccgc
    53581 gtgtgttatg ttaatgtgca ttgaaaatgg attttgccag tgcgccggat ttgaacaggg
    53641 agaaacagag gataacggta taggctgcaa gggaaaaaat cgcgctgtgc aggttatccg
    53701 ctaagaccat ttcgcctacc aaaactgcgt aaatgccgac gcatatcata ataagaaaac
    53761 cttgaaaacc aagtgcaaac agccccttta ggtagttgtt cccaatctgt ccccattccc
    53821 ggttggtcat agtggcaaac gggatagggg aaacggaaca gtaagcgtat atctcaatca
    53881 ttctgccgta gaggataacg gttatcagta cggacatgat tttcatacaa aggcttacaa
    53941 ggctcgtttc catgacaagt aacagtagtt cggggatttc catatctttt agtccctcct
    54001 gcatggcggc aagggcttcg gtcacgtcga tattcgtatc gccgccgatt acacccgccg
    54061 caccggacac aatatgctgc accatatcaa agactgccat tgttatatca aaggtatgcg
    54121 ttaccaagaa aacggcaaca aaggctttga aaaaccattt gaaaaataga aatgtgtcaa
    54181 cgtcgtgcat attgtttttt tccgttatca tgctgataag ttcataacag aggacatagg
    54241 taatgacaag ccccgcaatg ggtacaatca cattttccga cagattttgt atcatgttga
    54301 aaatgtttgc gttccacccc tgcggggtct tgcctacctc cgctgcaata gtgcctactt
    54361 tttcgtttac gtccccgaac atagtggaaa ggttgccgtt aatggctccg ataaggattt
    54421 cttttatcca ttcgttaatc gcttccagta tgctctccat aggctaccct ccgtaacctt
    54481 atccgaacag accggaaaga agcggtacaa ggatagtgcc gataagggca acaccgccgc
    54541 ccgccatgag ctgcttaatg ccctgtgatt ttgcacccgg attgtcgttt ccatatccct
    54601 ctaagagatt gataacgccc caaataccaa gtcctgctcc aagagcgata accaaagtct
    54661 gtaatacgcc gattgcgctg ttgaaaaatt ccataaaact cctttctgcc gcaaatgcgg
    54721 cttgccccgt aaaagggcat aaaaaacggc ggtcagtttt caaactgccg cggtcataag
    54781 tccccgcgcg gcgtaagtgt ctgccgcgcg gcggtattca gttgtccgtg taggtaatag
    54841 gaatttttgt aggggcacgt attctgtaag ttgtcttttt ctttgttact gtgtcaattc
    54901 ctcctttcgt tgctgcgcat acagggcttc acaccgtttc acaaactgcc gtatagcagc
    54961 ctgccgctgt tgttccctcc gttcagcaat caaaacaagc ccgtttatgg cttctgtgat
    55021 tttcttgtct tttttctttc tgatacgtct attcatggtc ggtgtcctcc tgcaaatcgc
    55081 tttcgctgat ttcgtagtag tcaaatactt cgtccggctt cacaagggcg ggtctgcgcc
    55141 ttaaatgctt ttccatatca aaagtatttt tcttgtcata gtcggaaaga tatttatagt
    55201 tggggtgctt tgtaatgtcg tacttatcgg aaaagaacgg gcgcacccct cgtagctgta
    55261 aaatacattt cccgccgtcc ataaccgcta tttcatcttc cgtcataagc tgcttgccta
    55321 acttctgata gttcagccca tgcgatacct cgcgcccccg gttctcggaa gtgttgaagc
    55381 tgtcaatggt ttctttcccc aagatttccg acatttcttt aagggtggtt ttctccttgc
    55441 cgcccaagaa aagggtggtg tcgcagttgc cgacaatggt atcggcgttg tccttataga
    55501 ttgcctttaa ctggctttgc gactgtaaga taatcgacgc ggagatttcc cggcttcgta
    55561 tggtggctat gagcttttca aactttggta tctgcccgat atttgcaaac tcgtctaaca
    55621 gacagcgcac atggacgggc agcctgccgc cgtattcatc atctgccttg tcgcaaagca
    55681 gattgaaaag ctgcgtgtaa agaatactca caacaaagtt aaaggtatcg tcggtgtcgg
    55741 aaatgataac aaacagggcg gtcttgcggt cgcctatggt gtcaagctcc atttcgtcgg
    55801 tttccataag gtcgcgcagt tccttaatgt caaaaggtgc aagccgcgca ccgcaggaaa
    55861 tgaggataga gcttcttgtc tttcccgcag atagcaggaa tttcttatac tgccggacgg
    55921 caaagtgttc cgggtctttt tcctccaacc gttcaaacat gaggtcaacg ggggactgaa
    55981 attccgggtc gtcctcgcgg gcttcactgg catttatcat ttcaagcagc gtcgtgaagt
    56041 tcttttcttc ctccggggct tcgtaccaaa tgtagccgat aagggcgcag taaaagagcc
    56101 gttccgattt tacccaaaaa tcctccccgg ctttttcccc gtcccctttg gtgttggcaa
    56161 tcaaggtatt taccagtttc aaaatgtctt tttcgctgcg gagataggca aagggattgt
    56221 atttcatact ttttttgaag tttattgtgt tcagtacctt tatccgatac ccgccgcgct
    56281 gtaagagctt cccgcactcg actaaaaccg tacctttcgg gtcggttaca acgtagcttg
    56341 agtgcatttg cattaaattt ggcttcacaa aaaatctcgt cttgccgctg ccgcttcccc
    56401 cgataacaag gatatttttg ttccttgcat acttcggctg cttcgggcgg ctgttcatgg
    56461 taagccgttc cgtctgtgtc agcagcacgt tattctgaaa taccgggtcg gtgtaaggct
    56521 ttatatcttc ggcgttgccc caccgggcag agccgtattc gatacctttc cggtatttct
    56581 tcgcgttctt gcctttcaca tagacaatca gccggataag cactgcgcct acaatcccta
    56641 tcagtaaatc cataggggca aggttcggaa gtgggtttgc aaaagcagcg gaaaagccgt
    56701 ctgcaaggga aagtaccttg ccggaaaggt cagtccccgg agagagctgc accgcctgcc
    56761 cgactttatc aaagagatag ataaagagca gatagggaat gttgggaagt atcagctttt
    56821 tccagttgat agcgttcatc ttgtaatccc cctgtctttg ttttttactt tgtcccgctg
    56881 tgcgttcaga gctgccgcct tatctttcag agtggaaagt agcttacgga tagagggtct
    56941 ttgttcctgt gtcagcttct ttgcggaaaa ctccttaaaa gctgcggtca gtgcgtccgc
    57001 gtcccgtccc ttgaaaaaca caaaatagcg gggcggggtt tcggtactgt ctttttttag
    57061 ggcaaagtca atcccgtatt ttttcgcagt atgttcaaag gctttgatat tgtcctttgt
    57121 gatttcaatg ttggaaacgc ccgcgttctg cttcataagc tgctttaggg tctgcttgcc
    57181 ctgcgggatt ttctgtttgt catgctgctt tttcatttgt gcaagcaggg ctttcatagc
    57241 cttttggagc tgctgcgccg tcagcttccc ggtcttaatg tagagggcta tggtcttttc
    57301 gttggtttcc tcctgcaatg gttttcctcc tttcttgtcc tatggtggta tgcccgccgc
    57361 tgggaagcag ggcgggcatg aaaaaagagc gtccgttttc acgccgccct gttaaatccg
    57421 taccaaaagc ccggagaggt ctgccgcccg aatacccaca agataccagt tatccccgta
    57481 ctccatacgg gttggcttcc atttgccccg cacaaaaacc tccatgcagt cgccgcagtg
    57541 taaccctccg taatagtcgt ttacgtcaaa gcgaatgtcg tagcggtcag cggtttcatc
    57601 aaaaatcaaa gctcctgttt tctgtgtcat aatcaaatcc tcctttggtt ttacaggctt
    57661 tcgccctcgt tgcagaaata ataatccggg tctccatact gcggcttctt tggtgtcagt
    57721 gcgccgcttg ccatgtcgtg tgccacaagg gacgtgtaat aactgttcat ggtagacggg
    57781 gcattaaaaa gcaccgcccg caggtattgt ttgatgttgc ggatttttgt ggtgttctcg
    57841 ctcatgcagt taaggacaaa ttcaatgtgg ctgctgtcaa gtttcataaa tcttgccttg
    57901 acaagctctg ccgggtaatc gtccccggcg atacggattg tctttcgttt ggtgcagaca
    57961 gtttcaagca tgagtgtcac aatttcattg atgaggtctt tatcaaggcg gttgttctgc
    58021 ataagaatgt catactcgat attgtccttg atgatttcct cgtaaatatc aaatgcgctc
    58081 ggttccgttt cctttcgctt ccgttccggc ggctgtgccg cgtcctctaa gggagaggga
    58141 tttagggaat ggaaaggaat ggaatgggta cttgatatat ctttatttaa tttttctttt
    58201 tttggtaagt tagtcttttg tatatcttta tttaattgcg ttggattttc cgacgtcggg
    58261 ttatccggta tcggattttc caatatcggc ttatccgctg ttggattttc caataccgga
    58321 caatccaatg ccgggggctg tggctgttcg taaatggtgt actcgatagc ggtcattttg
    58381 cctttctcgt cgcgtccctg ccgtcttgtg atatatccgg ctttttcaag ctcccataca
    58441 gcggtgcgga tagcgtcgat actttcccgg ttgataaggg acagtcctgc aagggtatag
    58501 tcccaatctt cgggaagtga gagcatttgc gacaacaagc cctttgcctt taaggaaagt
    58561 tccttgttgc gtaaatggtg gttgctcata accgtataac ctttgttctt ttccacgcgg
    58621 aaaactgcca tagcttcatc actcctttgg ttggatttgt tacgcagtag aaacgcagtt
    58681 ttgagggaaa aggtatcaac ccttgtcttt ctcatttcca tgctctaaga gccgcataaa
    58741 tcaaggcttt gtttgccctt actgcgtaac atgagggtaa aaaaagcagc gttcctgttc
    58801 tcctgtgtag gagtgagaaa cgccgcctta tgcggtacat tattcagttt ttagtacaat
    58861 acctgcttgt ctgttgctcg aaaaacattg attttcttgt attttcaaaa gtatcgttat
    58921 ctaaccctat cttttgaaag accgagtttc cagagaatga ttgaagatat agaggcagga
    58981 aaaatcaact gtgttgtaac gaaagacctt tcccgacttg gcagaaatta tattatgaca
    59041 ggacaatata cagaattgta ttttcccagc cataatgtcc gttacatagc gattgacgac
    59101 ggtgtagaca gcgaaaaagg cgaaagtgag attgcaccat ttaagaatat catcaatgaa
    59161 tgggtggcaa gagatacaag ccgtaaagtg aaatctgcat ttaagacaaa atttgcagag
    59221 ggtgcatatt atggggctta tgctccgtta ggatataaga aacaccccga catcaaagga
    59281 aaactgctgg ttgatgagga aacaaaatgg attgttgaaa aaatcttctc cctagcctat
    59341 caaggttacg gtagtgccaa aatcacaaag gtactgcgag aagaaaaagt tccgacagcg
    59401 tcttggctga attttacaag gtacggtact tttgcacata tctttgaggg aaagcccgaa
    59461 agcaagcgtt atgagtggac gattgctcat gtcaaggcaa tcttaaaaag tgaagtctat
    59521 atcggaaaca gcgttcataa tcgacaatca acagtttcat tcaagagcaa gaagaaagtg
    59581 cgtaagcctg aaagtgaatg gtttcgagta gaaaataccc atgagccgat tattgataag
    59641 gaagtgtttt atcgtgtgca ggaacagata aaatcaaggc gtagacagac aaaggaaaaa
    59701 gcaacgccga tatttgcagg gcttgtcaag tgtgcggatt gtggctggtc tatgcggttt
    59761 gcgacaaata aggcaaataa aacgccttac agttattatg cttgcagtta ctacggacag
    59821 tttggaaaag gtacttgttc tatgcactat atccgttatg atgtgttgta tcaagccgta
    59881 ttggaacgat tgcagtattg gactaaggca gtacagcagg acgaagaaaa ggtgttgaat
    59941 aagatacaaa aggctggcaa tgcagagcga atacgggcaa agaagaaaaa ggcaagtgct
    60001 ttgaagaaag ccgagaaccg acaaaatgag attgaccgct tgtttgcgaa aatgtatgaa
    60061 gatagagcct gtgagaagat aacggaacga aacttcatca tgctgtcggg gaaatatcaa
    60121 aaagaacaga tagaactgga acagcagata accaacctaa gagaagaatt aagtaaaatg
    60181 gaacaggata tgataggtgc tgaaaagtgg attgagttaa tcaaggagta ttccgtacca
    60241 aaggaactga cagcaccgtt attaaatgca atgatagaaa aaatccttat tcacgaagca
    60301 acaacgaatg aggataacga aagaatacag gaaatagaga tatactaccg atttattgga
    60361 aaagtagact gataaagagg gttcatatct ttaactaagg gaaactggag aatctattcc
    60421 tgatatatgt agttgcatta aacttgcaaa attgtacaat gtcaaactag atgacttagt
    60481 taatcattca gaagaaaaaa caggtattat agtacctcct aaaggaaagt ttttctttgg
    60541 agcagttgta gttggtgaac gtggtcagat tgtcattcca aaagaagcaa gagaagtatt
    60601 taatataaat gcaggagata aattattggt acttggagat gaggaaagag gattaggaat
    60661 tgttcatcaa cgtgatttaa taaattttat aggtcatgtg ggagtagtac gtaaagaaga
    60721 atgattttaa aaaattataa aaggaggtat gaaatatgag taaaatagta ttttttagca
    60781 ttcctgcata tggtcatact aatcctacta ttgaagtagt tcgtgaatta gtggatagag
    60841 gaaatgaggt attgtattat tcttttaatg aatttaaaga taaaattgaa ggtgctggag
    60901 caaaatttat ttgttgtgat aaataccttc cagaattact ccctggagat gagaaaaaaa
    60961 ttggtaagga ttttcctgga ttaattgaga tgatagtaga tactactatt tctttagatg
    61021 aaaaagtttg tagggaattt aaagatttta atccagattg cattgtatca gattcattaa
    61081 gtttttgggg aaagttattt gcacaaaaac ttaatattcc atatgtatgc tctacaacaa
    61141 cttttgcatt taataaacat acagcaaagt tgatgaaaca aaactttata gaaattatac
    61201 gtatgatttt tggtgtaaga cgcattaata aaaaaataaa gcttttacaa agaaaagggt
    61261 atgaagtaaa aaattttatt tctatagttt ctaatgataa tgatacggat accattgtat
    61321 atacatcaaa agaatttcaa ccaatggtag aaaccttctc tagtaagtat tcatttgtag
    61381 gtccatcagt atcaaagttg attattgaac ctaaagagag aaaaagaaag ctaatatata
    61441 tttcgcttgg tacaattaat aataaaaatg ttagctttta taaaaactgt ataaatgcat
    61501 ttaaagattc tgatgttgat gtgattatgt ctgttggtag aagtactaat attaaaagtt
    61561 taggaaatat accaaataat tttgaggtta aaaatagtgt tgaacagatt gctatacttc
    61621 aaaaaactga tgtgtttgtt actcattctg ggatgaatag tgttaatgaa agtttatatt
    61681 atggagtacc tatggtatta tttcctcaac attcggagca gagaatggta gctaagagag
    61741 ttgttcattt gggagcagga attatgttaa aagaggataa gtcagaaagt attaaaaaag
    61801 cagtatttca agttataaat gacaatgaat ataaagaaaa tgcaactaaa ctatcaaaaa
    61861 gcttttataa tgcaggaggc tcaaaaaaag cagcagatgt aatattacag attatatgta
    61921 atagtagaca agttgacata taatagattt gttaaaaata tatatacata catgtaattt
    61981 aatcaaagta ctgacagtgg ggtcttttaa cctaccagtt ggggcatata tattgatgct
    62041 aaatatgagg gatgttatat tttaataaaa aagataacat ccttttttta gtgcattata
    62101 tcgagtttta aataataaat gaaggaggat taatatgaaa aaatataaag ttatttttat
    62161 agtattaatt ataggtatta taagtgtaac agggtttgtt gtatataaaa actgcattaa
    62221 accatggaaa gatgctgaaa catatattga taagtatatg gtaaaacaag atatttcaaa
    62281 agataatata aaaagtatta caaaagaaaa agccaagaag gcaagttacg aaggaatact
    62341 atacaaagtg tattacaaag atgaccctcg atatgagtat caatattttt atagtgataa
    62401 ttattatgct ctatatgtaa ataaagtaat gttacaaatt tatgatatgg aaaataataa
    62461 taaactttta agaagta
//
LOCUS       DAEMHZ010000006        60619 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000006 DAEMHZ010000000
VERSION     DAEMHZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.006"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            25..471
                     /locus_tag="KPK72_000355"
     CDS             25..471
                     /locus_tag="KPK72_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2487602.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            473..1567
                     /locus_tag="KPK72_000356"
     CDS             473..1567
                     /locus_tag="KPK72_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG2487603.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMTKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1643..2173
                     /locus_tag="KPK72_000357"
     CDS             1643..2173
                     /locus_tag="KPK72_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2487604.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2501..3334
                     /locus_tag="KPK72_000358"
     CDS             2501..3334
                     /locus_tag="KPK72_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2487605.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYVYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIALLIIGMYKLFKKY
                     N"
     gene            complement(3585..4961)
                     /locus_tag="KPK72_000359"
     CDS             complement(3585..4961)
                     /locus_tag="KPK72_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487606.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            5229..6401
                     /locus_tag="KPK72_000360"
     CDS             5229..6401
                     /locus_tag="KPK72_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2487607.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKEGLCAFNMETGLVDNSLCIGRKGRIGCNIAVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6415..7062
                     /locus_tag="KPK72_000361"
     CDS             6415..7062
                     /locus_tag="KPK72_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487608.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVTSIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            7064..7573
                     /locus_tag="KPK72_000362"
     CDS             7064..7573
                     /locus_tag="KPK72_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2487609.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7937..9049)
                     /locus_tag="KPK72_000363"
     CDS             complement(7937..9049)
                     /locus_tag="KPK72_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2487610.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIKIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            9347..9613
                     /locus_tag="KPK72_000364"
     CDS             9347..9613
                     /locus_tag="KPK72_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG2487611.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9858..11585
                     /locus_tag="KPK72_000365"
     CDS             9858..11585
                     /locus_tag="KPK72_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487612.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11585..13375
                     /locus_tag="KPK72_000366"
     CDS             11585..13375
                     /locus_tag="KPK72_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487613.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13537..13845)
                     /locus_tag="KPK72_000367"
     CDS             complement(13537..13845)
                     /locus_tag="KPK72_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487614.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(14107..14655)
                     /locus_tag="KPK72_000368"
     CDS             complement(14107..14655)
                     /locus_tag="KPK72_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487615.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14758..15429)
                     /locus_tag="KPK72_000369"
     CDS             complement(14758..15429)
                     /locus_tag="KPK72_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487616.1"
                     /translation="MVTFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15825..16730
                     /gene="garR"
                     /locus_tag="KPK72_000370"
     CDS             15825..16730
                     /gene="garR"
                     /locus_tag="KPK72_000370"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG2487617.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            16866..18248
                     /locus_tag="KPK72_000371"
     CDS             16866..18248
                     /locus_tag="KPK72_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2487618.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEGDDSSKVPGFGISMFSLLFPILLILISNAFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            18319..19755
                     /locus_tag="KPK72_000372"
     CDS             18319..19755
                     /locus_tag="KPK72_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG2487619.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19941..20795)
                     /locus_tag="KPK72_000373"
     CDS             complement(19941..20795)
                     /locus_tag="KPK72_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487620.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(21063..21386)
                     /locus_tag="KPK72_000374"
     CDS             complement(21063..21386)
                     /locus_tag="KPK72_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2487621.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21589..22218
                     /locus_tag="KPK72_000375"
     CDS             21589..22218
                     /locus_tag="KPK72_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487622.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22530..23039
                     /gene="ruvC"
                     /locus_tag="KPK72_000376"
     CDS             22530..23039
                     /gene="ruvC"
                     /locus_tag="KPK72_000376"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG2487623.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            23032..23634
                     /gene="ruvA"
                     /locus_tag="KPK72_000377"
     CDS             23032..23634
                     /gene="ruvA"
                     /locus_tag="KPK72_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG2487624.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23741..24760
                     /gene="ruvB"
                     /locus_tag="KPK72_000378"
     CDS             23741..24760
                     /gene="ruvB"
                     /locus_tag="KPK72_000378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG2487625.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            25078..26103
                     /gene="queA"
                     /locus_tag="KPK72_000379"
     CDS             25078..26103
                     /gene="queA"
                     /locus_tag="KPK72_000379"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG2487626.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEFLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            26115..26600
                     /locus_tag="KPK72_000380"
     CDS             26115..26600
                     /locus_tag="KPK72_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487627.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26679..27800
                     /gene="tgt"
                     /locus_tag="KPK72_000381"
     CDS             26679..27800
                     /gene="tgt"
                     /locus_tag="KPK72_000381"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG2487628.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27934..28227
                     /gene="yajC"
                     /locus_tag="KPK72_000382"
     CDS             27934..28227
                     /gene="yajC"
                     /locus_tag="KPK72_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG2487629.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            28285..28647
                     /locus_tag="KPK72_000383"
     CDS             28285..28647
                     /locus_tag="KPK72_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG2487630.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28713..28856
                     /gene="scfA"
                     /locus_tag="KPK72_000384"
     CDS             28713..28856
                     /gene="scfA"
                     /locus_tag="KPK72_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG2487631.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            29483..31378
                     /locus_tag="KPK72_000385"
     CDS             29483..31378
                     /locus_tag="KPK72_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG2487632.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYIAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            31840..33243
                     /locus_tag="KPK72_000386"
     CDS             31840..33243
                     /locus_tag="KPK72_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG2487633.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQDVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKSGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      33415..33500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            33624..39587
                     /locus_tag="KPK72_000387"
     CDS             33624..39587
                     /locus_tag="KPK72_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG2487634.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEETPKEEGNISKDDNSTTSTYSKSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKILTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMARLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGEPGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDAKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGRLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            39797..41677
                     /locus_tag="KPK72_000388"
     CDS             39797..41677
                     /locus_tag="KPK72_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG2487635.1"
                     /translation="MSKRKSFIRTIAVSTMAVVVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRSE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASIKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            42044..43645
                     /locus_tag="KPK72_000389"
                     /pseudo
     CDS             42044..43645
                     /locus_tag="KPK72_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
     gene            43864..45453
                     /locus_tag="KPK72_000390"
     CDS             43864..45453
                     /locus_tag="KPK72_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG2487636.1"
                     /translation="MKVNKRVLSIGLTISLIMAGVPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEALQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            45678..47897
                     /gene="slpA"
                     /locus_tag="KPK72_000391"
     CDS             45678..47897
                     /gene="slpA"
                     /locus_tag="KPK72_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG2487637.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAANEFVADSNYTVVQGKYQKV
                     ITGLQDALKDGKISKIDVIFDGSSIGEVEPGGDPAAAAVKLKNLVDDKLDNLGDGKYV
                     QFNVAYSTTSVITKAELKNYYNKLDKEKILVSNEPTTTVNGLIKADQSPNQANGVAAN
                     EPLKLSDVITLSYDDEAGTLTATQNATNVVSNKVKDLKFGTATTGSYTAGKEITVPAT
                     ATITAGTSTTADFNLDDPSKAFKFDPSSAEISGFVTGQAGQTLKASIKIINAKEESID
                     VDSSSHRTAEDLAEKYVFEPADVNAAYEALNDLYKEGLTSDLITQDGGKYQVVLFAQG
                     KRLTTKGATGTLADENSPLKVTIKADKVKDLKDYVEDLKNANNGYSNSVVVAGEDRIE
                     TAIELSSKYYNSDDDNAITKDPVNNVVLVGSQAVVDGLVASPLASEKRAPLLLTSAGK
                     LDSSVKAELKRVMDLKSTTGVNTSKKVYLAGGVNSISKDVENELKDMGLKVTRLSGDD
                     RYETSLAIADEIGLDNDKAFVVGGTGLADAMSIAPVASQLRNSNGELDLKGDATPIVV
                     VDGKAKDINSEVKDFLDDSQVDIIGGVNSVSKEVMEAIDDATGKSPERYSGEDRQATN
                     AKVIKEDDFFKNGEVTNFFVAKDGSTKEDQLVDALAGAAIAGNFGVTVDNEGNPTVVG
                     KKASPAPIVLATDSLSSDQNVAISKAVNDDANTKNLVQVGKGIATSVISKIKDLLDM"
     gene            48144..50489
                     /gene="secA"
                     /locus_tag="KPK72_000392"
     CDS             48144..50489
                     /gene="secA"
                     /locus_tag="KPK72_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2487638.1"
                     /translation="MSVIDAILDKADEQEIKKLNVIVDKIDALEESMKKLSDEELKDM
                     TAIFKDRLKKGETLDDILPEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLDMTVGVIISNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLNAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMLGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            50904..52775
                     /locus_tag="KPK72_000393"
     CDS             50904..52775
                     /locus_tag="KPK72_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG2487639.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEVFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESINEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNIERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            52882..53589
                     /locus_tag="KPK72_000394"
     CDS             52882..53589
                     /locus_tag="KPK72_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2487640.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKARKIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKEFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPENGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            53622..55454
                     /locus_tag="KPK72_000395"
     CDS             53622..55454
                     /locus_tag="KPK72_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2487641.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKGIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTKNKNDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVTQIGGLGNEDVVDNIIDIQEKTKYTV
                     ETIEEFYVAMKKADANDVIIFETKKDDSINDSFEVKTTKAITVEFDGIFKKTITIDMP
                     NGDVNNFGEISDDIRINNIRKNTLINEGSIQGIDIYSKNGCKIENKSDGDIWLITIVS
                     EARGVYIVNDGDISKISNSCSDVIIKNSGKINLVTGTEEPAISGKKPITNDTEYDDER
                     AHGLSVKTEACSTPQKNYIIVTISSKPKNSNYAIYYRVVGDKPSAMYVGEKINPRDWY
                     SVSKSDDSFIEKAKNGSYIEVVEINSSNNRVSRWGRSSSTDDGL"
     gene            complement(55498..55899)
                     /locus_tag="KPK72_000396"
     CDS             complement(55498..55899)
                     /locus_tag="KPK72_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG2487642.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDIIVKAFTNIVVIVLNYFFSKMIIFKNKK"
     gene            56127..58538
                     /locus_tag="KPK72_000397"
     CDS             56127..58538
                     /locus_tag="KPK72_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG2487643.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEVYLKLKGYGTYDLSEEHLRWWATGGKYGWDLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDLK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            58835..60412
                     /locus_tag="KPK72_000398"
     CDS             58835..60412
                     /locus_tag="KPK72_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG2487644.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLSDALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT        24241 a         6870 c        10749 g        18759 t
ORIGIN      
        1 ataattatag aagggaaatt taagatggat tttaaaaatt tacagtatga aagtttgtca
       61 tatattattt gtaacttgca aaaaaacttt aaactttatt gtgaaaaatg tttaaaacca
      121 tataaactta cgaatggtct atatttctat ttaatttata ttaataaaaa taggaattgt
      181 tctttgaatg atgtttctac agaatttgaa gttgataagg ctcatacaac aagaactata
      241 tctaggttag aacaagatgg atacatagaa aaaatccaaa accctaatga tagtagagct
      301 tttcagttga gagtaacaga taagggtgag gaagtattag gggatataaa aaacatattt
      361 tcaaagtggg ataatcacat aaaaaaagag ttctcggata cagaatataa agaactggta
      421 aagaatttac atgtggtaaa ggatataaaa acagctgtgg aggaagaata ggatgtattg
      481 taatttatta aaaccaatta aaattggaaa tttggaatta aaaaatagag tatcttttgc
      541 acctacaagc atgggtctta aattggaaga aaaaattaaa aagttttctg atattgctaa
      601 gtcaggagta gctctgatta ctctaggaga tgtatctatt agacctagtt ttcataaggt
      661 agctataagc ctatcggatg aagatggagt catgaaatac aagaaaattg tagatgaaat
      721 acacaattct ggtgcaaagg tatcagcaca actattttgt tcagattatg atgttaattt
      781 aattaaagat actatgaaga tgggaattac ttcacatgat gagataaaaa aaataatgaa
      841 tgacggagta aaagattaca taacaaatat gaccaaagaa gaaattaaaa atattataaa
      901 tttatttaaa gtaacagcat tgaatgcaaa aaaagcaggt tttgacatga ttcaaattca
      961 tggtgatagg cttgtaggaa gtttttcatc aagtatattt aataatagaa atgatgaata
     1021 tggtggaacg tgtgataata gaagtagatt tgcaagcgaa ataattagta gtatccgaga
     1081 tgaagttaag gacattccaa tagactataa atttgcaata agacaagaaa atcctcatta
     1141 tggaaatgca ggagttttac tttcggaagt tgagtatttt gttaaaaaat ttgaatccct
     1201 aggtgtaaac agttttcatg taacacttgc aaatcattca aaattagaag atactatacc
     1261 aaccaataat catccttact ttaaagatga aggctgtttt ttatatttag ctgatgaggt
     1321 aaagaaacac acaaatcttc cagtatgtgg ggttggaaaa ttgtctagcc cagattttat
     1381 agaaagtatt atttcaaata atagagtaga tatggtttct atgtcaagac aattactagc
     1441 agatagtaat tggttacaga aagtaaaaga tggaagagta gatgagatta aaaagtgctg
     1501 ttattgtaat aagaagtgtg cagatgcttt acaaacacgc tctcagtttg gatgtatact
     1561 agattagaat atccttataa aaataaataa tttgttaagg ttaatatatt tttggtgatt
     1621 tagataaaga ggaggagaca tcatgaaaag tttagtttta tattcaagtt taactggaaa
     1681 tactaaaaag attgcttata ctatatatga tgaaattcaa gaagaaaaag acattaaaga
     1741 tgtaaatgaa ttagttgatt atagtattga ctatgaaaat tatgatatag tattcttagg
     1801 ctattgggtg gacaagggaa tttgtgataa aaattcaaaa caagtgcttg aaaatattca
     1861 caataaaaag atagcactat ttggaacaat gggagcaagt aaaaaaggaa gttatggagc
     1921 tagtataata gaaaagatag aatcaattat cccaaaagat aatgaaatat taggttcatt
     1981 catatgtcaa ggtaagatag cagaaggatt gaaagcaaaa tataaagaga tgttaaaact
     2041 ttctcctgac aacgaacata ttcgccaaca attaaataac catgaggaat ctcaaagtca
     2101 tccagatgaa caagaaatat atgaagccag tatgtttgcc aaaaatatga tgattaaagc
     2161 tagtattgtg tagtagttaa acaataagtt catataagat atagaataaa taatttagta
     2221 aaaaagtata gcaaataatt tgctatgctt tttttatgaa atcaaaaaaa taataaaaat
     2281 acttgactgt aaaccttgtt tatacattat tttatagaaa ataagatagt tttaaatata
     2341 aaaaaagata tattattgaa aggagatatt attgtatgaa aacttataaa agataattag
     2401 tttgatttta agatatacta aatatacaat tttatagagt gaaaatattt aagactatat
     2461 tatagttata aattaataga atagagaggt agtgtttaaa atgacaatta aagaagttga
     2521 agaaaaaaca ggattgacac gctctaatat tcgtttttat gagaacgaga atttaattaa
     2581 accccttcgt aatgagcgta atggctatag agaatattca gatgaaaatg tagaggacat
     2641 caaaaaaatt gcatatttac gtacattagg aattactatt gatgatatcc gtaatattat
     2701 attacagaag gtatctttag ttgaagttgt agagaaacag gagaaagttc tcgaaactaa
     2761 aatatcagac ttagaaaatg caaaaactat gtgtaataaa atgttagaat ctgatgagct
     2821 aagttatttg aatttgaaag tagaacaata cataactgag ttgccagaat actggaataa
     2881 aaacaaacct gtttttaaac ttgactctgt aagtttctta tatatatggg gagggaaaat
     2941 tacctgggga attattacta ttgcttgttt gttgattgca ataacagtat ttccaaagtt
     3001 gccacctcaa attcctatcc aatggaataa tggtgtagct aattatgtag ataaaaaatt
     3061 tatttatgtc taccctgtca tttgtgttgt attcagaata cttttacgtc catttatatg
     3121 gagatggtta caaatacatg ctttttatag tgatactatt gcagattatt taacgaattt
     3181 tatgtgtttt gtagcgttgt ctatagaaat cttctctata ctttttattt acaatattgc
     3241 cagacatgtt acaataatta tttttataga tatacttgta tttattgctt tactgataat
     3301 aggtatgtat aaattattta aaaaatacaa ttaattttta aaaattaagt aataagtgat
     3361 agtgtaaaaa gtagccactg caaattatgg attgtagtgg ctattttatt atttgtatat
     3421 gaaattattt tgtttttcta ataagttacg atatattatg gtttatttca ctgataaaag
     3481 gtttaggtat tttaaagtaa aaagttgtat attctaataa aatttaaaca ttttgattct
     3541 ttaaaattag agaaaataca ttgcttttat taaaaaatta aatattaaga atttaatttc
     3601 tgcatcttat ttagaaaaga taagaaatca actttatata tttttttggg ccttcctctt
     3661 tgattatcga gttttgtatt tacaatagtt gcaattcctg ctttatataa gatactcaat
     3721 attctattag cacttctaag agttatgttc atatagtctg ctaatatttc tgctgacaca
     3781 ttatttgaat ttctatcatt aatcatacac atgattttag atacatttgt attagataga
     3841 ggtataaaag aagcaacttt attggctatt gagctatctt caatattatt agaattttta
     3901 tttgaatata aagggcctat tgtatcatta gtatcattta ctataaaagt acagtttcca
     3961 ttcaaagaag cagatttacc attagcttga cgagcatttt gctctgcatg aatatttgta
     4021 tttccaatgc cccaacctat atttaagcta tatttaattt cttttaaatc ttctgaaata
     4081 aaaaaatctg tatagttatt ggtcatattg tttaacattt ctttagtagt gtgtatctct
     4141 attttcatat catttttttt gacctgaact ccatagaatc catgtcttga tagagaattt
     4201 aatatagtgt tatatatttg attttgtatc tcatcatttt taaatatcaa atcttcattt
     4261 atgtactctg aactatttac tgtaactata caagttgcag atttattttt gtctaagttt
     4321 tgtaatgtta tttcattcac cagagattta aatgtattca ggattgattc tgtagatgga
     4381 accatgtaaa tacaatcata gccaagctta ttaagttcac aactaatgat gtaatttcta
     4441 gtaaaagaaa ggttaatctt attatctcgc aataagtcta aatgtatttc caataattta
     4501 tcatttaatt ttttaaagtc atatacaggt tgagggatat taaaatcttt aattgtatat
     4561 ggacatttat tagagtctat taatgaatat atatcataat aattgttgtc ctctcttaaa
     4621 aaatccataa atgattttgt aaagtcaata tctttttggc tatttaataa tttgaataag
     4681 tatttataaa aatctctttc atcaagcttt aaaaaattta atggaacttt tatgtctttt
     4741 atatttatta taagttcact atatggaact ataccactgc atacaaatcc atcaaaatta
     4801 tctacttgct catagtatat attaggtaaa ttattaaaag gattctcccc tttgagctca
     4861 ataaatgtaa tttcacaagt atcatttaat tctttaagag cttgtttaaa cttatctgac
     4921 ataaaataag atgttgtcaa aaaaccaatt ttatatttca taaaacctcc tttaaaattt
     4981 cgagagtatt gtaactaatc aatcaattta taacctcatt ttataatagt agataaaacg
     5041 cgtcaattta ttttacaaaa ggataattga aagaaaaatc agaaaatata aaaaagactg
     5101 aaaaatatgt tgactggcaa gaataattgt gctattttgt aattaaggac tagtctttta
     5161 agatttccta aaataaactt aggttttagg aaagaaatac attttatttg aaaataaagg
     5221 agaagattat gaatacatat ttaaatgaaa tttctaagtt tgttgatgag aatagagaag
     5281 aaatagtttc attatggaaa gagattgtta atatggaaag ctatacacat tgtaaagaaa
     5341 gtgtaaacaa gttagctgag agattaaagc ttgaatttga aaaagaaggt ttagattgcg
     5401 aattagtaga tgttggagat aatggtagta cattgatagg tacacttggt tctaatatag
     5461 ataaaaagcc aataattttt tcaggtcaca tggatactgt ctttgaaaca ggtacatttg
     5521 gagagaatcc atttaaaata atagaaggaa aagcatatgg cccaggtgtt ttagatatga
     5581 agggtggtat aataatttcc ttatatgtta ttaaagcatt aaataaaata ggatacaaag
     5641 aaagaccaat aaaaatagta ttttctggag atgaagagat aggacataag gattcaacag
     5701 gtgcagatgt tatattaaga gaagcaaaag aaggtctttg tgcttttaat atggaaactg
     5761 gattagtaga taatagttta tgtataggta gaaaaggaag aataggatgt aatatagctg
     5821 taaaaggtgt agaaacacat gcaggtaatg attttgaagg tggaagaaat gctatagaag
     5881 aaatggctaa taagatacta cgcatacaaa aattgactaa tttagatgtt ggaactacag
     5941 taagtgtaag tgtgattaag ggtggaagag tggaaaattc tattccagaa gattgtagta
     6001 taaaagtaga ccttagattt gaaaaagtag aagaaatgga gaatgttaaa gaacaagtta
     6061 gagagatatg taaagaaact tacatagaag gaacttctac agaagttaat tttataagtg
     6121 aaatgatgcc atttgaaact acagaagatg ttatgagatt tcatacattt gtaaatgaag
     6181 tgtcaaaaga aaatggattt ggagaattaa atgctaagag acttggaggt agttcagatg
     6241 caagttattt aactattgca aacgttccta caatctgttc atttggtgta agaggggaat
     6301 ggaaccacac atcaagagaa tatgcagtag tagattctat gtttgagagg gtgaaattaa
     6361 tttctacagt tattttaaac ttagataaat ttgaggtata aagggggatt ttatatggat
     6421 gtaacagctg gagataataa ggtaacatgg aaagggtatt tatctttatt tgtattgata
     6481 gtattatttt caggagtgtt taaagatagt caaggcttcc ttaaagcatt agattttgct
     6541 aatctgacag gttcatttgg tactattgca gatggtgtag actttatggg taaaggagga
     6601 acgggagcaa aagatggttt cttatttgct ctgactttga ttccaactgt ctgttttgct
     6661 gtaggtctta tagatgtagt tgaatctatg ggaggaatga aagcggcatc aaagatattt
     6721 aatccattgc ttagacctct gcttggtata cctggaatag caggaatagc atttgtatct
     6781 tcatttacta gttcagatgt aacctcaata atgacgaaag aattatttga aagtggggaa
     6841 ataacagatg atgaaagaac tatatttgta gcatatcaat atgctggttc agctgttata
     6901 ctgaacacaa taaatactca agctccactt ttaccaatag cattattggc attaggtcca
     6961 ataatcctta tagagatatt ctgtaaaata cttggtgcta atctagttag attaatcata
     7021 ggtgtaagaa ataaaaagaa taaggctaag gaggctgtgt agtatgagtc aacaaaatac
     7081 tggaaatgca attgtaaata gtaaacctaa gaaaactgct gttgagatat ttatgggggg
     7141 agcaaaaaaa ggtttttaca taggtgttga acagatatta ccagccatga taatgggtta
     7201 tgtaatagtg cagtttttac aattaactgg tttagttgat attttaggaa atatttttgg
     7261 accagtaatg ggtatttttg gactacctgg ggaatctgtt gtagttttga tagcagcatt
     7321 ctttgctaag gcagcaggag cagctacagc tgcaaactta tttgcacaag gtctaatcac
     7381 tgcaccacaa gcaaccatat taattatgcc atgtatgtta atgggtactc tagttggtca
     7441 ttatgccaga atagtgcttg tagcaaatgc cagtactaag tatagaatgt tactgttggc
     7501 agttcctata tttgatgcaa tagtggggat gttgatagta agagttttac tcactgttat
     7561 gggattaatg taaataacat aaaattataa cctaactata aatgatatat aagaattaat
     7621 atatacaagg attaatgagt agtaattgat atatagtaat aaactaagat agtaattaat
     7681 gtgtatatta attaaaaaaa gagagtatct caaagtgaac tttttagttc atgagatact
     7741 ctttttgtat gaaatcaagt atattttcat aatttcaaca atgaaagaga ggcttatctc
     7801 tattttgaga cagcctcttc ttttaaattt atatttctag aatttatgtt ttttatttat
     7861 ccaacattaa cattagttat tagaaataat tttaagaaaa atatactttc atgttaatat
     7921 cagaataaat ctgtttttaa aggatgattt gttttgaatt ttcacgaata tctacttcta
     7981 tgactttttc tattgtatct cctctaaaac ctaataaatt acttgtcaag tttgcagtgg
     8041 aacaagaatg attaggtata attcttattc tatcaccaat ttttatatca gtttcaccat
     8101 ttactttaat ttttccaact tcttctgaaa gtgaatcaat agtaagttct ggatgtccac
     8161 ataccctacc ataaccaaca atactactat ttccatgtgc acctttatca agtccaaggc
     8221 acttacttcc acaatcaatt aaatagtatc cactgctatt ttttgagatt acagtgcaca
     8281 caacagtaag agcacacata tcttcagtag cacaacctaa acctatctgt atagcatcat
     8341 agtatacata gtttccaggt cttacagcag ttatagtttt agaagcagct tcagattcaa
     8401 ctgttggagt actaccagaa gcgacaattt ttatattaaa tccattatca gttagagctt
     8461 tgtatgcttt ctccatagaa gaatcttctt gaacacaaac atctggaaca ccttctggag
     8521 aactacaacc atacacttga cctggatgcg aacttatacc aacaatcttg atgttttcta
     8581 gcttagaaat ttcagtagca agttcaacca ctttttctgg ttcaacccca aaacggtgta
     8641 agccacaatc tacaataata agtagcttgc actctatttt tgatttagaa aaagcttcac
     8701 tataagcctt agcacattct atattatctg tagcacagat taaatttatt tttgatgcca
     8761 tagtcacaat tctatttata tttacttctc ccatgtaagg ataagccaac ataatatctt
     8821 caaaacctgc ttttatcaga gcttcacctt catcaagtgt tccaactaga aaaccactag
     8881 cacctgcatt tttttgaagt ctcgctatgt aagtactttt atgtgtctta agcataggcc
     8941 aaagttgttt attattattt gtacaaattt gttgaatttt atctatattc ttttctaact
     9001 ggtctaaatc tagcaaaaaa tttggagtag cgacttctga aattttcatg ttatttacca
     9061 cctttcaaca aaagtaaatt aactataagt attataatta ttatattagt aatatcggct
     9121 ttataatatt aatagcttat aattatatta tagaactagc gttaacagag agcaatattt
     9181 atttgtaata attagaattt tcaataagtt agtgtggttt taaatttttt atataatata
     9241 tttttaattt tatgcaaagt ttatttaaat tagtttgagg atttattaat agggtattat
     9301 tgaatgagta aaaaagtgat gtttacatag aataatcaca ctataaatta atttagaacc
     9361 taaaaaatta attgagcaaa ttggaaaaga gaaggataaa aaagatattt ggatagttgg
     9421 tggaggcgat atagtaaaat tatttatgaa agataactta attgatgatt attatatata
     9481 tatattgcca ataatattgg ggagagggat ttcactattt aagtctggta tagataagac
     9541 taatttgaat tttaagaaag ctagtaatat tggagaactt gtaaaattgg aatacagtaa
     9601 aaaacaaaaa taaattacat gccaatttgt tacaatgtta tgaaatgaag catttaaaag
     9661 acttttggct atatgaattg acattacttg tcaaatatgt taacatataa gcgacttgtc
     9721 ggagggtgta ttaaaaagaa tatgctggag aagagagtgc gttaactctt ttctcctttt
     9781 tttataaaat taagcattta taaatttagt attattacat gattacacta tctcaaaaat
     9841 taagaaagga gaaaaatatg aaattaattt taagttattt aaagaattat aagttattaa
     9901 tagtattaaa catactggct atatttagtt ttgcattagt cgagttgggg attccaacta
     9961 taatagctaa aataattgac aatggtattg caaatcagaa tatagcttac ataaaacaaa
    10021 tgggaatagt gattgttatt atttctatta taggggttgt aggtagtata ttgttgggtt
    10081 attgttctgc taaggtatca acaagtgtaa ccagagatat aagaaatgac atatttgaga
    10141 aatcacaaga attttcacat actgaataca ataaatttgg aatttcttca atgattacaa
    10201 gaacaacgaa tgatgcattt caaattcaac aatttgttaa tatactacta aggactgcct
    10261 tattaactcc agttatgttc attataagta taataatgac aattagaaca agtgtggaac
    10321 tttctatagt attggctata tcagtacctt ttataattat tggagtagca ataatagcaa
    10381 aagtatctca ggcaatatct tcaaaacaac aaaaaggact agataaatta aaccttatat
    10441 cgagagaaaa tttaactgga ataagagtaa ttagagcttt tggaaatgat gattatgaaa
    10501 cagagcgatt tgaaaaaaca aatacatatt atgctaatgt ttctaagaaa ctatttaagc
    10561 ttatgtcaat tactcaacca gcattctttt tactattaaa tatagctgtt ttggctgtat
    10621 tttggatttc aagtgagaag ataaatattg gagagcttca agttggacag ttggttgcat
    10681 ttttggaata tcttttccat gccatgtttt caattatgct tttttcaatg gtatttatta
    10741 tgtatccaag agcagaggta tctgcaaata gaataaaaga actattaaat gaagaaccat
    10801 tgataaaaaa tcctgaaaat ggaattaagg atactgaaaa taagggtatc atagaatttg
    10861 ataatgtaac ttttacctat ccagatggag aagcttctgt acttaaggat atttcattta
    10921 ctgcaaaaac aggtgaaaca gtcgctttta taggaagtac tggtagtggg aaaagtactc
    10981 taattaactt gatacctaga ttttatgatg taacagaagg tagcattaag ataaatggag
    11041 tagatataag agaatatgat ttaaaagctt taagaaaaaa gataggattc atacctcaaa
    11101 aatcactgct ttttactggt agcatagcaa ataatattag atttgggaaa cataaggcag
    11161 gagaatcaga attagaatat tccgcaaaag ttgcacaagc atatgagttt atatcaaaaa
    11221 aacctagaaa gtttgatgaa ttgattagtg aaggtggagc aaatgtatca ggaggtcaaa
    11281 aacaaagact ttctattgca agggctatta taagaagacc tgaaatatac atttttgatg
    11341 acagtttttc tgcacttgac tttaaaactg atgcaatact tcgtgctaaa ttgaagaagg
    11401 aaacaaaaga tgcaattgta ttgattgttg cccagagaat tagttctatt attgatgctg
    11461 ataagattat agtactcaat gaaggacaag ttgtaggaat gggaactcat aaagaattat
    11521 taaaaaactg tgaaatatac tatgaaattg caacatctca gttaaaaaag gaggaattag
    11581 aataatgaaa aagtctaaag gttttaagaa aacaattggt agacttcttc catttgtaaa
    11641 gaaatataaa ttttccttta ttatagctat tatatgtata atttcagcag ctactatgaa
    11701 tgcacttgca cctaaaactg aaggattgat aataacacaa ttgactaaag atgtaattag
    11761 tatcgcaaaa ggagtaccag gggcttccgt aaattttgat tatgtaacaa aaatattggt
    11821 gattttagca tgtatatatt ttgccaatgc aatttttacg tatacttcaa gttttctact
    11881 tacgaatgct attcaaaaca caatgagaga tttaagaaat gaagtcgaaa ataaaataag
    11941 aagattgcct attagttatt ttgatagtaa tagttttgga gatgtattaa gtcgtatatc
    12001 aaatgatgta gatacaatat caaatgcatt gcagcagagt tttatgcaaa ttgtaaactc
    12061 aatcttagtc ataattttgg cactttcaat gatgtttaca attaatatat atatggctct
    12121 tatagctctt tttataattc caattagtta ttttgtatct aaatttgtag taaaaaaatc
    12181 tcaaagtaga ttttcattgc aacaaaatgc tcttggaaaa ttaaatggga aagtacaaga
    12241 aatgtatact ggatttaatg aaataaaact atatggtaaa gaagaagatt ctataaaaga
    12301 atttaaaaaa gtaaatcaag aactttgtga aaatggattt aaggcacaat ttatatcaag
    12361 tatgatgaat cctatggttt cattagtaac ttactttgga attgcagctg tggcagttgt
    12421 cggttctata tatgcagtaa gtggaggaat aacagtagga aatttacaag catttgtgcg
    12481 ttatatatgg cagataaatc agccactttc acaaatgact cagttatcta cagtaataca
    12541 atcttctttt gcagctatag aacgtgtatt tgaaatttta gacgaagaag aggaaatacc
    12601 tgatgttgaa aatccagtta agatagaaaa tgtaaaagga aatgttacat ttgagcatgt
    12661 taattttgga tatggagaaa attcaacttt aatagaagat ttaaatgcag aagtaaaaag
    12721 tggtcagatg gtagctattg ttggacctac tggagcaggt aaaacaacat taataaactt
    12781 attgatgagg ttttatgatg taaagaaagg tgctattaaa attgatggag tagatataag
    12841 agatatgaaa cgtaaagatt tgcgttcaat gtttggtatg gtgcttcaag atacatggtt
    12901 atttaatgga accatttttg aaaatataga atatggtaga tttggagcta ctaaagaaga
    12961 aattatacaa gcagcaaaag tagcaaatgt tcatcacttt ataaccactc ttccagatgg
    13021 atacaatatg tttttaaatg aagaagcatc taatatatct ctaggggaaa aacagctttt
    13081 aaccatagct agagctttta tatcagaccc ttcaatattg atacttgatg aagctacgag
    13141 ttctgttgat actaggctgg aacttatgct tcaaaaagca atgagaaatc ttatgaatgg
    13201 tagaactagt tttgttatag cacatagact ttctacaatt cgcaatgctg acttgatttt
    13261 agttatgaat aatggaagta ttatagagca ggggaatcat gatgaattaa tggaaaaggg
    13321 tggattttat gagaagcttt ataatagcca atttgcagac aaagaatcag aatagataac
    13381 ataaactttt gtataaaatt ttgtatcaaa ttcaaatatt aatcatataa ttaaaaagag
    13441 tatcagtaag ttgttttttt atagatttac tgatactctt tgattttaag actaaaggta
    13501 tttatgattg gtacttataa ttcacaacta gtcttgttac atctgataac cagaattaca
    13561 gtacttttct tttttacctt tttgttctct aacatatagc aattcgctta acttatcaat
    13621 gtaataacca tcatcactta tgcgaattaa actagtcaga taatatccat ctttgcttct
    13681 aggaatcttt ttatttattg ctaaatcaat agctgtcttt gctgcttcaa aatgctttaa
    13741 atgtgtatga ccttcttcaa aactctttct agtattataa actacataac cttttttaac
    13801 ttgaaaaact ataaattctt tcttttcata tattttatta aacattcaat cagcccttct
    13861 caagatagtt ttatagcata acattagaag cttctaaaaa attttacaaa gaacataaat
    13921 attttataaa ttataaatct aaaactataa tttataaaga taactttctg attatttata
    13981 tgataattca tatagattaa aagtaaatta tctttaggtt aaacaaaagt aaaataaaag
    14041 actagttata atatttatat gaaaaagtac aaaatatagt atatgaatat agaaaattta
    14101 aattatttat ttacagtttt tatttccata agtatattta ccttcagata ccttgtcatc
    14161 atggaaatat attctttcac aacttttatc tccccataga atgttttttg ttattttatg
    14221 ggaagtatct cctgtaaata cttcagctac agtagtatct gtaacatcac agtaaagaga
    14281 agcaaatttt ttaaaccaag gattttcctc aaatcttaaa agccaggaag ctccataggg
    14341 gcaatgtaac tcttcaaatt caggaaccca tcctagaaat ggaacatcag ttgtgctaat
    14401 aatattgtct gcaattaatt catctccatt ttcatgagct ctttctctca tagcaatagc
    14461 tctttcttca gacctttcaa ataaaacttt tcttaatatt tcgctagttt tatcttcacc
    14521 aaactcttca aataacactt tggaaaaata aaaatataaa tcaccaaaat gtttgcatgc
    14581 catacgaact tcttcaatag cataatcttt gtcaatagtt tcattagtta caacatcttc
    14641 gttcaaaaaa atcactcctt agaataagta ttagttacaa tataattata gaatattttt
    14701 tataaattag gaaattaatt tgagatagat atatttttaa taaatgaaag tataatacta
    14761 aaataactta ttgttacaaa aatcttctaa attaatgtat ttaggagttt ccatatgaat
    14821 gagagtagtt ctgtaaatat cttcaataca tccatttgca atattattaa aaatatctgt
    14881 gtgctctttt aatgttgttt caagccttcc ttcatgtgac aatgaatcga gagctaattt
    14941 ttttattctg tacatataat tgttaaacat tttgtcaaat tcagtatttt gagcatatga
    15001 aactatagtt gtgtgaaata atgtatcgta ttctgcaaat gagtttaaat ttttatcacc
    15061 taataaaata tctttttgtt tatctaaaag atgttttaat ttacttatag tttctactgc
    15121 tctagcttct ttgtattgac ttgtaattaa aaaagtacaa aatccttcta ttgcagagcg
    15181 tatttggaat atctctacaa tttcatttgc tgtaatttga tggattctaa atcctttgct
    15241 tgggattata tctataaatc catcttgact aagtctttga agagcatctc ttataggtgt
    15301 tctagagata cctatttcac tagcaagttt tgtttcagaa tatatttcat tttctatcat
    15361 ttctccagat agtatcaatt cttttaagta gtcatatgct tgttcttgta atcttttaaa
    15421 tgtaaccata cttaataata cccccctaaa caatataaaa atacaaaaac ttatataatt
    15481 ttaagcatac aacatagtga aaataaggtc aatcttttgc aatttaaatt gcagataaga
    15541 ttaaaaaata taaaaaatta ttgaagtgtg aagtatgatt aacattaaag gcagattaat
    15601 taaaactaat tgaaattagt taaaatacaa aactgtaaaa gtagtatatt atatttgaaa
    15661 acaatagtat gtattaaaac ttatatagga tgactaaata taataaatga ctttaaaaat
    15721 tactatttat tgttaaaata aatactttta tattttatat tgaccggtat actggtatgt
    15781 agtatactct aaaacataaa aaacgttttc aaaaatgtga agatatggag ggttataaaa
    15841 tgaaattggg atttattgga ttaggaataa tgggaaagcc aatggcaaaa aatttattaa
    15901 aagatggatg taatcttttg gtgtatgaca ttaataaatc tgctgtggat gaacttatat
    15961 catgtggagc aaaatacgca agtgttttag aaatgggtca agaatgtgat attgtattta
    16021 caatccttcc aaatggaact attgtgcagg atatattgtt tgggatggat ggattggcaa
    16081 aaactttaaa agaaggaagt atagtagtcg atatgagttc agttactcct acagaatcca
    16141 tattgtgtgc aaataaatta aaagatatgg gcttagaatt tatagactca ccagtaagtg
    16201 gaggggagcc aaaagctatt gatggtacac ttgcttttat ggctggtggt aaggaagaaa
    16261 tttataagaa agtagagcca ttttttaata taatgggaag ctcatctatt ttaattggag
    16321 ataatggtag tggaagtgtt acaaaactta caaaccaagt aattgttaat ttgacgatag
    16381 cagcagtttc tgaggcattt gtacttgcag caaaagcagg tgcagaccca gaaaaagtat
    16441 ataaggcaat aagaggaggc ctagctggta gtacaatatt ggatgcgaag ataccaatga
    16501 ttatgaatcg tgattttaaa ccaggtggaa aaatatctat taatttgaaa gatattaaga
    16561 atgtaatgca aacagcacac aatttagatg taccgctacc tatgacaagt caattattag
    16621 aaattatgca gacattaaaa gtacatggac atttggaaga tgaccatagt ggaatagcac
    16681 agtattttga aaagttggca ggtgttgaaa taaaaaaaca cgataattaa tatttaaata
    16741 tatcaatata attaaataca tattattagg attagattat ataaatacag cgaggttaag
    16801 ttatcatagt aaaagaaaaa taaaattgat aaataataaa gtagaatgat aaaggggtaa
    16861 agattatgat tacaggatat ggacttttga tagcatttgt tatatctata ggaattttac
    16921 ttgtatcgat tattaaattt aaagtaaatc cgtttttggc attactgata acatctataa
    16981 ttacaggctt tatggttaga atgcctataa atgaaatatc aaccacaata tcgactggat
    17041 ttggaaatac attaggtagt ataggtattg taattggact tggaataata tttggaaata
    17101 ttctttcaga atcaagagca acagaatcta ttgcaaaggg gttgttagct aagactggag
    17161 aaaaaaacag tgctttagca gtaactacag caggtttttt aatttcaatt ccagttttta
    17221 tggatgcagc ttttgttata atgatgccta taattaagta tgtttcgaga gtaactaaga
    17281 aatctctaat ggtttttgta tgtgccttag gagttgggac tatagtaggt catgcacttg
    17341 taatacctac accaggacct ttagcagttg cagcaaatgt aaatgcaaat gtaggtagtt
    17401 ttatacttta ttctataatt gttgcttttc cagcagctct tataggtgga tggatttatg
    17461 gaaaacgttt tgaaaaatat ccagcttatg ctattgatga gaatgataga gaaaaaaatt
    17521 tagaacaggg aaaagatagc ataaaaattg aaggtgatga ttcttctaaa gtaccaggtt
    17581 ttggaatttc tatgttttct ttattgttcc caatacttct tatacttata tcaaatgcat
    17641 ttagtatgtt cttagaaaaa ggttctacaa tgagtggagt acttgcattt ataggagata
    17701 aaaacatagc aatacttctt ggaatattgg tggcaatagg atttttaaag aaatacataa
    17761 ataaacctat gggagatgta gttattgaag ctgctgattc agcagggcta atattattga
    17821 taacaggttc aggtggtgcg tttggcagtg ttataaatgc aagtggtata ggtaacttct
    17881 tagttgatac tatgtctggt cttagtatat cagttgtagt gcttggattc ttattaagtg
    17941 ccttgcttag aatttctcaa ggttctgcta ctgtagcctt agtaacaaca tcatcaattt
    18001 taggacctac aattttggca actggaatgt caccagtttt agtagggctt gctatatgtg
    18061 ctggaggagt tggtttctcc cttccaaatg attctggatt ctgggtactt tcaagattta
    18121 gtggattatc agttaaagat actttaaact cttggacaat tggaggaacc attgctggag
    18181 ttgtagcatt tataatggtt ttattgctta gtgttataaa tggaattata ccacttccag
    18241 gtctataaat aacaaactat tgtttatatc atataaatta catttagact atacaaatta
    18301 cataaagggg cgttttaaat gagagaagta aaaccaatta gcatagatat acttaatact
    18361 tttaaacaag tagatgagga tagattaaat aaacttcttg cagatgagct taagcattta
    18421 gatagaaaaa ttgttgtatt agatgatgac ccaacaggtg tacaaacagt tcatgatatt
    18481 tctgtttata ctgattggga taaggatagt atggaacaag gttttaatga gaaaaatagt
    18541 atgtttttta tacttaccaa ttcaagggga tttactgttg ctcaaactac aaaagcacat
    18601 aaagaaattt caaagaatat tgtggatgta tcaaagaaag ttaataagga ttttataatc
    18661 ataagtcgta gtgattctac tatgagagga cattatcctc ttgaaactaa tttattaaaa
    18721 agcgaagtag agagattatc ggggaagtta tttcagggtg aaataataat gccatttttt
    18781 aaagaaggtg gtaggtttac tatagataat gtacattatg ttaaagaagg agaaatactt
    18841 gtaccagcag gaatgactga atttgcaaaa gataaatctt ttggatataa atcatctgat
    18901 ataggtgagt ggtgtgaaga aaagacaaat ggaaagtaca aatctagtga tatgatatat
    18961 atttcgtcaa aagaacttag agagctaaat attgaagcta taacagaaaa acttaaaaaa
    19021 gctgaaggat tcaataaaat tattgtaaat gcaaccgatt atgtagatgt aaaggtattt
    19081 acaattgcgt ttatacgagc agtaaatggt ggtaaggaat ttatgtttag aagcgctgct
    19141 gccataacaa aagtacttgg cggagtgagt gacaaggaac tacttactaa ggatgaatta
    19201 gtatcaaaag gtaataccaa tggaggaatt atacttgttg gttcacatgt gaataagact
    19261 acccaacagt tagaagaact taaaaattgt aaatatccga tagaatttat agaatttaat
    19321 caacatttag ttcttcaaga aaatggtcta aaaaatgagg taaaaagagt tataaaagtt
    19381 gtagaggaaa aaataagcag gggtaaaact gttgctgttt atactagacg agagcgtttt
    19441 gacttggata cgaatgataa agataaacag cttatgattt ctgtagaaat atcagatgca
    19501 gttaccagta taattggtat gcttaatgta agaccaaatt ttattatagc taagggtgga
    19561 attacttcta gtgatgttgg tactaaagca ctaagagtaa aaaaagctac agtaatggga
    19621 cagataaaac ctggtatacc agtatggatg acaggcaaag aaagcaaatt tcctaatatg
    19681 ccatatataa ttttccctgg aaatgtggga gagattagca cacttagaga atcagttgag
    19741 atattgatgg ggtagtgtat tagtgtattg aagtttaaac gcttaaattt actgagttaa
    19801 aattaaaata aactaaaatt ataaattaag attaaaaata atagtatatt aattagggtt
    19861 tagagtattt tacaaaaaat attctaaacc ttaatcttat gtattagtat ttttattatt
    19921 taacttatgt gtttatttta ttatttatct tttttaattg atttaatatt ttctttgatt
    19981 ttatttaaaa tatctgtatg cttattagag cttatgttgg ggaatgcttt gattatacgt
    20041 ctatgaagta atttgtggtt agacagcaaa gtattaagtt tattttttag ctcatcaata
    20101 cgatttttag catagcgata gttttctttt tctttaagta tttcaaacct atcttccaaa
    20161 gtatccagtc tattttcaac taaagattca agtttgtttt ctatagtttc ttgtgtaata
    20221 agtttgtagt attctttctt atcttttaat tccttaatta taagattgat ttgttttaac
    20281 ttaccattta attgtaaaat tgtatggact gtgataacta aatctaaaac aaaataaatt
    20341 tctatagata tagcaaatat aaatagcaca tttttagaga tagagttaat aaagtcaacc
    20401 acaataggat gtactataag cattacaatc aatgataata caccaaacat aagtgagttt
    20461 tccagacaaa ctcttccatg aatattaaat ttatgtttag aataatccca ccaagtcata
    20521 ttaaatattt tttctaaaat aaagctcgta caatactcca ttatgctagt tataattata
    20581 ctaaacaaaa ataacaaagg aatattgtgt acaaaaggag tcaaggtagc aactattgct
    20641 aatgctccaa atccatatat agggcaaaat ggaccattta aaaaacctct atttacaaat
    20701 tgtttttgta aaactgaaca atatgtagtt tcacaacacc atcctataaa gctataaatt
    20761 acaaaataaa gaaattgaat ataaaatgta ctcataagca cctcatttct agttagtata
    20821 acactatata ctcgatattt tatttttatg attaatttac taaattcaat ttacatattg
    20881 attataccaa tttatagaaa tttatcaaat ttaattacag ttagaacatt ataaacatgg
    20941 ttataataat gaaattttat ttaaaataaa taagggtgtc aagtataatc aaactaatag
    21001 ttgtagatac tctacactat gttagtttaa ttacatttga cacccttata tttatactat
    21061 gactatttac attcgaaact agagcaacat gtttcaatat aaagatttac tctatcagtt
    21121 tcaggagcaa ccaatatatt atcaagtgta cataagctac catctttgtt gtatttacaa
    21181 ttttttacag tacaaactaa ttcctcacaa tttctattat taacgttgct agataaatta
    21241 ctataaatag attcatctaa aaatgaacta caacaagtat gtctgtcatt gtgagctttt
    21301 tcaccattta taaaaatttt atctgcataa catatatggc tgttgttatg tgaacagtta
    21361 attacattac agtttattct ttccaaagtt attacctcct aaacgattat ttattataaa
    21421 ttatgtccat ataattacaa gaatataacc attaagagaa aactaccttt tagaaaatat
    21481 ctattcttag agtaatttga aatataaata tattttcttc taaaatttac ctaaattgta
    21541 taaaaactta cagcttggag aaaataatag aaaaaggagg cgtaatcaat gtttgaaaag
    21601 aagaaaaaag aattatttcc ttggaaaata tctgccttaa tactggttgg agtatttgta
    21661 ttaagtagcg gagtttatat agggacaaga ttaaaacaca atggagttca aactggtgaa
    21721 gttgtaagta ctagtgcgaa tatgaatgaa aagtcaaaag aagcttttgg attaaataaa
    21781 aattgtgaag tttggcttca aagtgaaggc gaaaatgaaa ctaactataa gagaacactt
    21841 acaatggcag gaatagttcc agagtcatta ttagataaga cgaagtcaga aatagtatca
    21901 tattttaaaa aagaatatcc aactaaaaaa ataaagagta tggataaaaa tgaaatcata
    21961 ttggtagaat ctaaatatag tgataataaa gatgtacaag caatggatgt tactataaaa
    22021 ggtaaatata ctattgaaaa tgataatgga aatatagcac tatataaata tgatgatagc
    22081 ggaagtaaaa cattagtaga aaaaacaaaa ataagagtag attcacttcc taaaacagtt
    22141 caagatgaaa tcaaaaaagg tgtagttatg gatactgaag aagaggccta ttcaagatta
    22201 gaagactttg caagttaata gtaaattaat attattttaa gttaaatact aagtaaatta
    22261 ataataaata atttaatatt aaatagatta gtattaaaat tgtagtcaca tttaattcct
    22321 ataaaaatac aataatttat ttgtaataaa tataaaagtt atattgaggt aaaatcttgc
    22381 tttgatataa cttttttatt tgattttata ttttacatag agcattttat gatttgttaa
    22441 tattatttat gatataatga tttagaacat aagtttttat gagaaataca ttttggtatt
    22501 tcttttattt ttagggggaa ttttaaaaaa tgataatatt ggggattgac ccaggtatag
    22561 ccatagttgg atatggtata attgaataca aaaatagcaa gtttaaagta atcgattatg
    22621 gagcagttac aacacctgcc catatgaata tatcgagaag attggaactt gtgtataaag
    22681 gaattgatac aatagtaaag aattacaata tagatgaagt tggaatggaa gaattattct
    22741 ttaacaagaa tgtaaaaaca gctataacag ttgcacaagc tagaggtgtt actatgcttg
    22801 catgtgctca taatgggaag cctgtatatg aatacactcc acttcaagta aaacaaggtg
    22861 tagttggata tgggagagca gataaggcac aagttcaaca gatggtaact tcatttttaa
    22921 gtctaaaaaa agttccaaaa ccagatgatg ttgcagatgc tttagctgtg gctatttgcc
    22981 atgctcattc aaacaaactt gaaaaaactc taaagaatat aggtggtaag tatgtatagt
    23041 tatataaaag gtacagtaga agaaatttat atagacagta tagttgttga aaataatggg
    23101 ataggatata aaataaatgt atcatccaat acaataatga atttacaagt gggagaagcc
    23161 acaaaaatat atactaaatt aattgtaaga gaagatgata tgagtctttg tggttttgta
    23221 agtagggaag aacttaagat gtttgaatta ctaacatctg tatctaaaat aggacctaaa
    23281 gtggcgcttt caatactatc atttgcatca ccagcacagt taggagcata tattttaagt
    23341 gaagacattg gaaaattatc caaagctcct ggtgttggta aaaagacagc agaaagaatc
    23401 gtcttagagc ttaaggataa ggtagacaaa aataacatag agtttgaacc aacgttatta
    23461 tcacaaaaac ctaccttgat atcacaagat gagtctgtag atgcacttgt agctttaggc
    23521 tatactttgt cggaatcaaa agaggcagtt caaaaatgta aaaaagatgg tatgaataca
    23581 gaagctatta taaagaaagc acttacatat ataatgagta aatccttgaa atagtatttg
    23641 gattttataa tatttggatt ttaaaacaat atttaaggag tttatgtaaa tttgaaaata
    23701 tataaagtag attttcaaaa gtacgagagg aggggtataa atgcaaggtt ttgaagatga
    23761 aaataggata ataacttcta caatgaagat ggaagatata gatatagaaa acagtttaag
    23821 accaaaaact ttagaagatt atttgggtca agaaaaatcc aaagaacaac ttagtatatt
    23881 tatagaagca gctaaatcga gaaatgagca gttagaccat gtgcttttat atggaccacc
    23941 aggtcttgga aaaactacac tagcaagtat aatagcaaat gagatggggg taaatttaag
    24001 aattacgtca ggacctgcaa tagaaagagc tggagatttg gcagccatac ttacgaattt
    24061 aaatgaaaat gatgttttat ttatagatga gattcataga atcaatagaa gtgttgaaga
    24121 agttttatat ccagcaatgg aggatttttg cctagatata ataataggga aagggccatc
    24181 tgctagaagc ataagacttg accttcctaa atttacactt attggagcta ctacaagagc
    24241 tggtatgctt acaaatccac ttagagatag atttggtgtt atttgtaaat tggattatta
    24301 tacagttgat gaactttcaa aaatagtact taggtcttca agcatcttag atgcagagat
    24361 acagtctaat ggagcattag aacttgcaaa acgttcaaga gggactccaa ggatagcaaa
    24421 tagattacta aaaagagtta gagattttgc acaagtcaga gctgatggaa aaataactga
    24481 taaggtggca aaagatgctc tggaactttt aggagtcgat agtttgggtc tcgattttgt
    24541 agatgagaag cttcttatga ctataataga aaaatttaga ggaggaccag tgggtttaga
    24601 tacattggca gcttctatcg gagaagatag aaatacaata gaagatgtat atgagccata
    24661 tttgcttcaa ttaggattta taaatagagg tcctagagga agagtagcta tgccattagc
    24721 atatgaacac ttaaaaattc catatcctaa tgaaaaataa aaatagatta tacgagtatt
    24781 tataaaataa aagagtcatt aaattaaaat atttctaaaa tggctaaaaa ttcaaacata
    24841 tccacaaaat ggtaattata tctgtagata ctgtggataa acacgataaa aattgtgtgt
    24901 attgtatcta tatattgtgg ataagacatg tatatcgtgg aaaaatatct atatattgtg
    24961 gatatggtgt ctgttaaaaa tagcaatttc ttgatagatt atggtaatat tgtggaaaat
    25021 aagttgtttt tttgatatta ttgtagctgg agaaatttta agagaggaag tttaacattg
    25081 aaaacaagtg attttaaatt tgacttacca caggaactta tagctcaagt tcctatagaa
    25141 gatagagcta gctcaagatt gatggtatta gataaagaga caggtaatat tgaacataaa
    25201 gtatttagag atataataga atttttaaat ccaggagatt gcttagtatt aaataatact
    25261 agagttatac cagctagatt aataggagag aagcttgaaa ctggaggtaa aatagagttt
    25321 ttattattaa aaagaacaga ggaagatact tggcaggctt tagtaaaacc aggaaaaaga
    25381 gcaaaagtag ggactaaatt ttcttttgga aatggaaagc taattggaga agttgtagat
    25441 ttatcagatg aaggttcaag aattataaaa ttccattatg atggaatttt tgaagaaata
    25501 ttggatgaac ttggaaatat gccactacct ccatatatca ctgctagact agatgaaaaa
    25561 gaaagatacc agactgttta ttcaaagcat aatggttcag cagcagcacc tacagcagga
    25621 ttacatttta cagaagaatt gttaaataaa ataaaagaaa aaggtgtaga tatagccttt
    25681 gtaactctac atgttggctt aggaactttt agaccagtaa aagtagagga tgttttaaat
    25741 cacaagatgc attcagaata ttatatggtt agccaagaag cagctgacaa aataaataga
    25801 gcaaaggaaa atggaaaaaa tgtaatatgt gttggtacta ctagttgtag aactatagaa
    25861 tcagcttgta atgaggatgg aaagatgaaa gaaactagtg gatggacaga aatatttata
    25921 tatccaggtt ataaatttaa agttttagat aaacttatta ctaattttca cttaccagag
    25981 tctacactaa taatgttagt aagtgctatt tgtggaaagg ataatgtttt aaatgcatat
    26041 aatgaggcag taaaagaaag atatagattc ttcagttttg gagatgctat gattataaag
    26101 tagggaggta atggatgcat agtttacaaa aacaaaaaaa gaataaccct atattaaaga
    26161 ttgcttttat atttgaacgt gttttagcag tagtagttct aatagcagta tttttaggaa
    26221 ctatagatgt tctaagactt atgtggggag catatatagt tgattttcaa aatccagtac
    26281 aatacagtca gcttaatgat tttttagcac aaatattact tttagtaata ggtgttgaac
    26341 ttgtagtaat gctatctctt catataccag gagcatttat tgaggcatta ctttacgcaa
    26401 tagctagaaa aatgttgcta ctgcctaaaa atgaaggtat gatagatgtt ttaataggag
    26461 ttatagctat agcaggacta tttgctataa aaaagttttt agtaacaaaa gacaagagtg
    26521 ctcttaatat aatgagtatt catgatgaag aagcaattaa ggaacaacag aacaaagaag
    26581 acgaagataa atctatttag acaataaatt atatgaatag cttattacaa atatatctta
    26641 agaaataatg agaaataaat atgattaagg agaactttat gtacgcagta agatacgaat
    26701 tgataaagac ttgtaagcag agtggtgcaa ggttaggaag attacacact ccacatggga
    26761 ttattgaaac accaatattc atgccagttg gaactcaagc aactgtaaaa tctatgacac
    26821 cagaagaatt aaaagagatt ggttcacaaa taatacttag taacacatac catttatata
    26881 tgagaccagg tcatgaactt ataaaaagag ctggtggtct tcataagttt atgaattggg
    26941 ataaaccaat acttacagat agtggagggt tccaggtatt tagtctaggg cctttaagaa
    27001 agataaaaga agaaggtgta gaatttaggt cacacttaga tggttcaaaa cattttttaa
    27061 ctccagaaaa agctatggaa atacaaaatg cattgggttc agacataatg atggcatttg
    27121 atgagtgtgc accataccca tcagatagag aatatgtaaa aaattcttta gaaagaacaa
    27181 caagatggtt aaaaagatgt aaagatgccc ataataatac cgacaagcaa gctttatttg
    27241 gtataataca aggtgggatg tataaagact taagggaaca atcagcgaaa gaaataacta
    27301 atattgattt accaggatat gctattggtg gacttagtgt tggagaacca aaaccactta
    27361 tgtatgacgt gttagagcat acaactccac ttatgccaaa ggataaacca agatacctaa
    27421 tgggagttgg tagtccagat gacttagtag aaggtgttat aagaggagta gacatgtttg
    27481 attgtgtcct acctactcgt atcgctagaa atggaactgc aatgactagc caaggtaaag
    27541 tagtagttag aaatgcaact tatgctgaag actttactcc acttgaccct gaatgtgatt
    27601 gttatgcttg taaaaactat tcaagagctt atataagaca ccttataaaa gcaaatgaaa
    27661 tactgggtgc aagattaata actactcata atcttcactt tttattaaat ttaatgaaac
    27721 aaataagaca agcgataatg gaagatagat tacttgattt tagaaatgaa ttttttgcaa
    27781 aatatggata tgaaatatag gttttgcact attaattata atcattttaa tatataatat
    27841 tcaatagtac tcaaagatag agaaagtaaa ttgttatata aacatttatt atgatttaca
    27901 aaagagtata atttacaaag gagtgaaaaa ttaatgccac agcaacaaat aattatgagt
    27961 ataggtctat gggtagtagt aatcgcaatc ttttatttcc taatgattag acctcaaaag
    28021 aaaaaagaca aacaattaaa agaaatgaga agtagcctaa gtgtaggaga caaggtcatt
    28081 acaataggtg gaatagtagc taatgtagct aaagttgaag atgatgttgt aatattagaa
    28141 ttaggaccaa atagaactaa agttccattt gaaaaatggg ctataggaac tgttaaatct
    28201 aaaaaagaag aaatagaaga agactaaatt agtaatttaa aagccttttc tcgaatatat
    28261 attaatttga gaggaggttt ttttatggga aaatctaatt atatttttaa aggtctagga
    28321 tatgcatata taataacttt ggctgtttta ttagtgtata acttattttt gacatttaca
    28381 gatattggtg gagataatat aactatggtc tcatcattta taactactat ttctgcagct
    28441 ataggagggt tttacacttc aaaacacatg aaagaaaaag gacttatgta tgggctttta
    28501 gtaggacttt tgtacatagt atgtattttt ttgactgtgt ttttagcaca agaaaagttt
    28561 gtatttgaaa tagggatgat ttataagtta ttgctaatat cagcagcagg aggcattgga
    28621 ggcgtattgg gtgtcaactt taaatagagt ttatattaaa gtagttttat gatataatca
    28681 ataggaaaca attagaaatg gaggcgttga aaatggaaaa taaacatgta aaaactttat
    28741 ctcaagctac tttaaagcaa agtgctgcaa aaggtggatg tggagagtgt caaacttctt
    28801 gtcaatcagc ttgtaaaact tcttgtacag ttgcaaatca agagtgtgaa agatagaaaa
    28861 ttattgattt agttgtttgt aagagggtat ctcaaagtga ttcttttatc attatgaggt
    28921 atcctctttt attttcaatt attatataaa aatatatatt ctatttatgt aaaattaaat
    28981 ttgatttctt atcgtttaat tatgcaaaaa tatctaatca tataagagca tttattttaa
    29041 agtagctact cttatatttt attttttatc aattacatgt attagaataa tttttaattt
    29101 taaactctaa tttatgatga tttttataat atgttatata ttttaatata tggagataga
    29161 tattaattta gtgatatata tttaagtaat aagagtaaag caaaaattag tgcatagtag
    29221 tatataaaaa tggagtttga atacttttgt tagtatatga ttgtttagta aaaaatcact
    29281 ataaaatagg atttctatct ttgagcataa ttatagagag cctaagtagg gggaagggtt
    29341 tcaagaagat attattagtg tttgtgttag taaattatgt aaacacataa aatatattga
    29401 aaaataatat tatatataat attatttatg tgtgggaaat acgaatataa aaaataaaaa
    29461 ataatattcg gaggacataa atatgttaag caacaaaaag agaagtatgg caatagtaat
    29521 ggcaggtgct acagttatga gtgcagcagc accaatattc gcagacaata ctgtgacaga
    29581 aaatgttgat aaaaattaca cagtaagtgc gaaagattct gctaaactaa tcgaagaggt
    29641 tagaaaagca ttagaagtta agtttgaaga tacaaaagca ggagcaaatg taaatgatag
    29701 agtttacgat ataaaagtag acaatgtaaa cttaactaat gcaactcaat tacaaaataa
    29761 aataaactct ttaacagaag ggcaaagttt aaaagttact attcaagata aaggacatca
    29821 agtgttaggt ggaaaagtag ttgactataa aattgaaaac tacaaaactg ctcaagaaat
    29881 agtagatgca gttaatgctt acaatgcaac tttagcagaa gattctgata acaagttaac
    29941 agcaactata aaatctacaa atacagttga agtaaaaaga gcaaaagatt cagcaaatgt
    30001 tattacatta aatgtaggag accaacattt agatttctca aaagtgataa catcagaaga
    30061 aggtactttt gaaggttatg aaaagagtta tagtgacata gattcaaaag aattacatac
    30121 gataacagtt aaaaacgcag atttacaaga catatcagca gaagaattat ttgatggaat
    30181 tagattaact acattaggaa gagaaatagt taataaagtt aaaaatggat atgctttaac
    30241 atttgaaaat gaagcaatcc ttactcaaga acaagaagat agtgatgata aagataaacc
    30301 agaaaaatca agctttgata tagttttaag caaggcaaat gaaaagccag aaacaataag
    30361 tgtttcaagt aaaaaccata agttagttag agacttacac aaagtattaa ctgatgtaaa
    30421 agatggaaaa gaattaaaag tagaagtttt atctggtgat tcaagattta caacagcagt
    30481 agaagttagt aaagagagat ttaaagatgg agaagcagaa gctataattt tagttggaga
    30541 agatgctata gttgatggat tagcatcagc accacttgcc tctcaaaaaa atgcaccaat
    30601 attattatct aaaaaagatt cactaccatc agaaatagaa gctgaaatat taagagtact
    30661 tggaagtaat ctatcttcta agaaaatata tatagtaggt ggagaatcta aagtatcaaa
    30721 agaaactgaa gaaaaacttt ctaaattagg tgtaagtaaa gttgagagag tttctggaga
    30781 agatagattt gaaacttctt tagaaatagc aaaacaatta aaagatacat ttaagactgc
    30841 ttttgtagta ggtggaaatg gagaagctga tgctatgagt atatcagcta gagctgctca
    30901 atttggtgct ccaataatag ttacaggtaa cgaattagat gcaaatgctg aaaaattatt
    30961 aaaaggaaaa gaattagaaa tagtgggtgg agaaaattct gtatcaaaag aagttgaaga
    31021 caaattagtt gatatagatt taaataataa agttgaaaga ttagctggag aaaatagaaa
    31081 agatactaat gctaaagtaa tcaataaata ctatgcaggt gcaactaaag catatatagc
    31141 aaaagatggt tatgtaggtg gaaatggaca attagttgat gcacttacag cagcaccact
    31201 tgcagctagt tcaaaggctc caatagtatt aactacagaa gaactttcta aatcacaaga
    31261 agaagtagtt gagttaagac ttaagaatgc tactaagtta gtacaaatag gtgaaggaat
    31321 agctaaaaac gctattgaaa aaatagcaga aaaaataaac ttatttacta agaactaata
    31381 agatatagga attaatatta aaagtttatt tagataatat atgattatac aatgcgttaa
    31441 tatgtaagac atagtgtaga tattataaaa tatacaaata acaagataaa ataataaaaa
    31501 taaggtgtct catttagaat tagattgaga taccttattt tattttttga ttataatgtt
    31561 gagaaattca tattgcaaaa attattataa aaagatactt cttataatga ttaaacatct
    31621 tattaacata aattattttg aacttataac ttaattaaat aaaataaatt ataatgtttt
    31681 gaatttatag agaataaaaa ctttaggtat attgtttgag ttgtatggat aactatggta
    31741 tcatatataa aatatggtaa caaaattgta acttttgatt aaaaataagt atgagtacta
    31801 ataaaaaata tgaaaagtac attgggggta gaaaataata tgaagaatag taaaaagata
    31861 ttagctatag gacttacact atttttagta atggtaaata ctcctatggt aaatgcgttg
    31921 acatcagttg agcaaataaa agggaatgac agatatgaga cagcagcaaa aatagcagat
    31981 aagcaaaact acaatacagc aatactaatc aactcagata atagcttagc agatggtcta
    32041 agtgcaagtg gtttagctgg agccttaaat gcgcctattt tgatgacaaa acaaaaccag
    32101 attccaaaca caactatgga aagattaaac aaagcaaaga ctgtgtatat aataggctca
    32161 gaatcaacaa taagtaaaaa tgttgagaat cagttactat ccaaaaagaa ggttgtacaa
    32221 agaatctttg gtgaaaatag atttgataca agtataaaaa tagctgaaaa aatcaaagaa
    32281 attaagccaa tagataaagt aattatagcc aatggattta caggagaagc agatgcgata
    32341 agtgcatcac cagtagctgc tagagatgga gtacctataa tacttacaga tggaaatagt
    32401 gtgggatttg acacgacagg cttaaagagt tatgcgcttg gttctagtga aataatcagc
    32461 gacgaattag ttaagagcac aaattctatt agattgggtg gcacagatag atttgaaact
    32521 aataagatag ttatacaaga gttttataaa aatagtaaag aattttatct aagtaaaggc
    32581 ttacaattaa cagacgcttt ggctgcctct acaatagcaa aaaatgctcc tgtagtttta
    32641 gttgaaaatg gcagtaacaa gagtatttta agtggtgcag acaaattgac tgtactagga
    32701 ggcataaatc aggacgttat caagcaatgt ataaatcaag cttcaccaaa tcaacaaggg
    32761 ttatattaca atccaaatga tagagcattt aaagagagaa taaagggtaa agtatatgct
    32821 cttacaaagc agtatagaaa agagaatgga gtaagagcgt tatctgtagc aagcagatta
    32881 gagggtctag caaatgattg gtctaattta atggcaaata agaagacttt atctcatacg
    32941 ataaatggaa aaaattcata ttctactttt ttgaaatact tagattggag tgagattaaa
    33001 tctggatata tagcagttca aggtgaaaat ataattaaat ataaaattcc agataaacct
    33061 gtatatacaa atagagatgc agatgatata ggaaacttta tttttaatga gtggaagaca
    33121 aatccagaag aaggcactaa tatgttgcat aaagggtatg aaataatggg atttggtata
    33181 gctataacag gagataagaa tctatatgct acacatgaat tttatggaag atataaagaa
    33241 taagaaaata tacacaaaat tatgctttta aggaagctat ataaataatt aaattataat
    33301 ggaaaataac atttatatta ataaattaat ataaatgtta tttttttata atttaatgta
    33361 aaaaaattgt attaaaaagt ttaaatatag tataaaatgg gtaagaaagt agtgaaaatt
    33421 aaatggcaaa attagggaaa tctaatgacg caaaactata gggattaaag cttttaaagc
    33481 catatcagcc agttgctaaa aagagtaaag tctttttatt ttataattaa gattctcttt
    33541 gagtgtcttt ttttattgtc tgaaacttat agtatttaaa ttcatgaaaa attagatatt
    33601 tattaaggga ggattgttat ttcatgaaaa aggcaatatc ttgtgtacta gcagtatcta
    33661 tgtgtagtgc accactaaac gtttttgcag aacctatatt agaggggaag ctaagggcag
    33721 ttgaaaaatc agttacagaa aagttaaggg ggagtcttga agtagattta aatttttcac
    33781 tgccaataaa aaatagcgag tcagaaagta tgactaatat gcagttacga ttaaaagatg
    33841 atagtaacaa tagtggaata ataaagcttg gagaaaaaga ttcaggaaca gtaaatgttg
    33901 ggacagataa tatagaatat actataaaaa aattatctgc aagtagaagt gaaataaaaa
    33961 ataaagatga gaatatatca tattataata ttgtatttaa taatttacct gttggaaaat
    34021 acaatgtaga agtctctgga gatggattta agaacaaaga aattaaagac attgatatat
    34081 caagttattc acaaagagtc ttattaagca atagagcaag tgtagataat aaaaataaac
    34141 ctttaaatta tgaactattc ttaatgggtg atgtaaatgg agacggtaaa gtagataaga
    34201 gtgattataa taaggtttta gagaatatag attcaaataa aagagaattt gatttaaata
    34261 gagatggaaa ggtagatatt gttgacttag attatgtaca gaaaaattta ggacaaagtg
    34321 aagatgcaaa aagcctagaa agtatagtat ctaccaatcc tatagtagat acaagtaagg
    34381 ttgaattaaa aggaaatagt gatgtagata ttaatggtaa tgtagaaaat ttatttagtg
    34441 gagaaaataa tggagttaca atatcttcaa aaaatgatga aatatctgag gaaaaaccag
    34501 cagtaatgga tatagcttta tcagaagcac ttaatatgga agtcatcaat ataaaagctc
    34561 cagaaggtac tgctccagca aaaggaatag ttgaggttac tgacaaagat ggtaatgttc
    34621 aacagattcc ttatgaaaac aaagaatata tgaaaaactt aaaagatgag gagcaacaag
    34681 aggaagtaaa taacgaacaa gaagaagtaa atcaagaaga agtaaataat aaagatgaaa
    34741 atataaacca gactgataat aactcaaata ataaagatga aaataatgat aaacaacagg
    34801 aaaatagcga taaacaagaa gcaaactcta ataccaatca agataaaaat aatgataaag
    34861 aaaattcatc aatggataaa cctaattcag atgaagagac tccaaaagaa gaaggtaata
    34921 tcagtaaaga tgataactct acaacatcta catatagtaa gtcagcatta gaaacatatg
    34981 cagatgttca aaatagcgat atagtaataa atcttggtaa acaagtagca gtaaaaaaga
    35041 taactataaa aataacagct acaacagcaa atgatagaaa tcttgcagaa atatcaaaag
    35101 ttgaattttt aaacaatgta tataaagaaa taccaaagcc agagatgaac atacctaaaa
    35161 taaataaaat attgactagc actgctgttg gaaatgaaaa agtatctttt tattggaata
    35221 atgaagtaaa tgtatcagca tacgaactta tactagacaa aattgatagt aagggaaaag
    35281 tattatctac taaaaaatta caaactagta aaaatacaat agaagttaga gatatagaag
    35341 catatggact atatagagtt agcatacaat ctttaaatgg agattggtct agtggttata
    35401 aagatgcaac accagaaaac ttagacggag taccagaaaa tgtagattca aactataaac
    35461 ctatacaatt tagcccagat agtatattag aatttcaagt tgttccagac acaaaaccag
    35521 aaccaccaga aggtataaat gttaaaggta aatttaaatc tcttgatgta agctggaaaa
    35581 atcataaaca agctaaagat tttgacttgt actataaaga aaaaggttct aatgggtctt
    35641 ggataaaata caatgataag cttattcgtg ggacaagttc aaagatagaa ggtcttaagg
    35701 ataatacaac atatgaagtt agaatgacag ctactaacca cttgggaact agtggaatgt
    35761 ctaaaacata tataggtaca actgaaagca tggatgcacc aacaacacct aactatagat
    35821 taattaatac tcctaaagag aatggtggat ttggaatacc aacaaatcac attgtagatg
    35881 ttacttatcc agcaggatat gacaaaagtg aatatagtga taaaaatcca tttgataaat
    35941 tcaatgtagt agatggggac tttacaacac attggacatt cccaacatat aatgcagctg
    36001 agggaacgaa tagagggcca ataataactt ttgataaatc ttatactatg gatactttta
    36061 tggctacacc tcgttttgat gtttctgata agacaataaa tgactttgag gtacgatatt
    36121 gggatgaaaa tggtcaatta cacaatctgg gggatgttcc ttttgaaaca aagacagacc
    36181 ctgtaaataa taataagtat gtaatggcta gattaaaaga accaataact gctaccaaga
    36241 tacaagtaag tgtttgttca tcctattcat ataagtctaa tatatcagaa cttaagtttt
    36301 atgagtatga cagtatagaa gatgatgtaa gaaatctatt tatggatgac ctacaagttg
    36361 aattaaaaga agatgtaact caagaaaaaa taactgaatt aaaagataga ttaaatactc
    36421 cagataaagc tagtggagaa tatcatccat ataagacagt tattgagaga gaattaaaat
    36481 tagctcaaga cttatataat gatagagcca cacttactga tgtaactact gtaaatcaag
    36541 aaataaataa attaggaaaa tataaagata aaaacggaca gtatactgta aattccaata
    36601 atttaggtat gcaaaatgac tggcaggcat taggagtagc tgctagagca ggagatgaaa
    36661 tcacagtata tgttggaagt aaaagtggaa aaactcctaa gttgatatat acacagtact
    36721 atggagaacc tggtgcatat aagagtggag aaataaatct taacactgga aaaaatgtaa
    36781 ttcaattaag caaacttcat agtttagata tagaatgtgg aggaagttta tacataagat
    36841 atgctgaaga tactccaagt ggaggagata taaaagtacg tgtagcagga gctactaaga
    36901 taccacatct taatctaaat ggaacaataa atgataagag tcctgcggga gtttctgaat
    36961 ctaagaaaaa gataaaagca tatatagaag aattagctaa atataaagat gctgtaaaag
    37021 gagaaccata ttatccagga acaaatgttg gtaattcata tggatattca gaaaaaactg
    37081 gtgtgttaaa cactacagat attgaaagtg ataaagtaac attaaatgtt cctgcaacag
    37141 cagtatatca agggatatca aaaggaaatg ctgatttaaa tacacaagta gatagattat
    37201 ataatagtat gcttgcatgg gaacaaataa tggatttagt ttactcagaa agaggagtat
    37261 ttaaaacaca agatttagat ggtaatggaa ctgtagatga taatgaattt aacttaacta
    37321 aaaatgatat ggctccaaaa tctcgtatga acataaaata ccaaagaatg tttattggag
    37381 catttatgta tgcttctgga ttacatgtag gagtagaata tggttcagtt ccagggctta
    37441 tgaatggagt acctttccaa atagatgata gtggaaaagc tacaggagga aacttatttg
    37501 gatggggaat aggtcatgag ataggacatg ttacagatat tggaaagatg acatatagtg
    37561 aaactagtaa caatgtactt gctttacttg ctcagacctt tgatgataag acacattcaa
    37621 gacttgaagg ttcaacaatg gataagatat atgagcatgt aacttcaaat agcttaggta
    37681 taccaagcaa tgtatttgaa aggcttggaa tgttatggca attacatcta gcttatgatg
    37741 atgattttac gggttcaatg ttaaagaata actcagatgc tgatttaagt aatgatacat
    37801 tctatgcaaa gatttcaaga aaatacagag cactttcaag tagtgaccca attaatagtt
    37861 taccaaaaga ccaaatgcta gtagcaatgg catcaagtgt tgttgaaaaa gatttaagag
    37921 attactttaa ggcatgggga gtagaaatta caccagaatt aaacagtata atggatagta
    37981 agaactacga aaaagaatca agaaagatac aatatgtaaa tgacgaagct agaagaaaga
    38041 ttttaaatga aaatatagct agtatggcaa aagatgctaa agtaagtgct agcttttctg
    38101 atggaataca aagtggttct ctagttaaat ctaataaaat atctatagac ctagatgtag
    38161 ataaggataa agataagata ctaggatacg aaataataag aagtgatgga aattatttag
    38221 atggtgaaaa tggaacacag gtaaaataca gaacagttgg atttgtggat gcaaaaggaa
    38281 atagtaaggc taaatttact gataatatat ctcctcttaa taacagagca tttacatata
    38341 aggtagttgc atatgattat cacctaaatc caacagaaga atttgaagtt ggcactgtaa
    38401 aattatcaga tgaagggaaa ataaataaat cagcatggtc attcataaca aatactgttt
    38461 ctgacggtga tgttagaact gaaaatgact cacatggacc attacaaaat cctgaaattg
    38521 ataatataaa agataatgat gcaagtacta catataaagg aaagataata agtaaagcag
    38581 agtggaacaa agaccctcaa aaagaccctg atataaatgt agaagaaaat ccatatataa
    38641 tagtagacat gaaagaaaca ttacctatag taggactaaa atatacaaaa ccagaggcag
    38701 aagctaggaa gttctcatta aaaggattat ttaactttag aaagaatact agcacaacat
    38761 acaatccttt aactaattac aagatacaag taagtaatga taaaaagaac tgggaagatg
    38821 taagtcaagg aacttttgaa tatggacaag atttagttgg tggaggaaaa gaccaagata
    38881 atgaagctag agttacattt aataaagatg gtagactttg gacataccaa gctagatatg
    38941 taaaactaat ctcaaataat aagtctaata tagaagttgc agaattagat ataataggac
    39001 ctccaggtga taatatagaa attggcataa taaataatgc agacgaaact cgtactaatg
    39061 gaattggaaa attagcagaa gattatgtat atcaaagtga tgactctagc acaagtgaag
    39121 atgaaacagg taagattcca gcaggttcaa tagttattac aggtgaatac agtggaaatc
    39181 cagcatttaa tttaccacta cttatagata aaaacaataa gactatatct ggagaagcac
    39241 ttttatttgc agaagttcca gaaaaaggtg aactaggtga aatcagtaaa ggtacatggg
    39301 tttactatat gccaaaagaa aactttgatt ctttaagtga taaggttaag gctgaactat
    39361 atagatacaa tgaccttcaa ggagatactc cagtaggtca aagatttgta agtgatacaa
    39421 tgtatgtacc tgtaggagct aagaattatg atgagttaaa gacaataagt cttactgata
    39481 gtaatagtaa agctagaaaa gctacaagaa attctattga tatcagtaat aagaaggtta
    39541 taactgtaag caataaagta aagggtaata ttgttagaga taaatagtag gtggaactct
    39601 aaaaacgtaa aaatactaac actaacattg tatatctaat aagattaggt actagaatag
    39661 ttttaaaata gtagatatta aaagtaaagt atataaatgt aaccataaat ttagggtaat
    39721 ttataaatta aatataaaag ctaggtggat aagtaaattt atccacttag ttaatgatag
    39781 ataaggacgg gtgttaatga gtaaaagaaa atcttttata agaactatag cagtatcaac
    39841 tatggcagta gttgtaactg gtagtgctac atgtgcatat gcagctccag ttttacaagg
    39901 aacaaaaacg tatgagaaag taaatactat agatatatct gtggactcag tagaaaacat
    39961 agtatattca tttcaagcca gtataaaagt acaaggtgag gttgaagtag tagataatga
    40021 gcagaaagaa aagattactt ggtctgataa tataaaatct cagataaaaa gtggaaatgc
    40081 aaatgctact tgtagatcag aatacaacaa atcaagtaat acaactacat tagatatata
    40141 tgttacatca aatgaagact tactagacgg aaatagattg aatataggaa gaatatctgt
    40201 aaaaaaatct ggcagtaact caaatgctga ctataaagtt ttaggaaaag gaacaagcga
    40261 taaaccagct ttaaaaattg taacatataa caacaaaaca gtggattatg aaaatataag
    40321 ctctgatgaa ggattaatat ttacccttat taatgagagt gaagtaaaac caattggtgg
    40381 aactggttct agtaaaaatg accctgaaaa gtataaggta gagaaaagtg aagcacttga
    40441 atatttatta aacaatatta ggataaacta ttctattgtt agtaaagaaa cacaagaaag
    40501 tggttcaaat gtaatattaa aacttggatt agcacaaaaa acaactaagg gaagaaaagc
    40561 tacaataaac aaatatgttg aagttacact tccaaaaagt ttagaataca tagttgaaaa
    40621 tgaattatca aaaccagatg aattaccacc agataatggc aatggtggaa ataatggcgg
    40681 aggaagtaat tcaggtggct cgtctagtgg tggaagctct ggaggaggaa attcaagtga
    40741 ttctacatct aatgtcacag tcaaaaaatt aaaaggtgct gatagatttg aaacagctat
    40801 caaaatatct caaagtggtt ggacaaaatc agatacagtt gtaatagtaa atggagaaga
    40861 taaaagtatg gtggatgggc ttacagctac accacttgct agtataaaaa actcaccgat
    40921 tcttttatca tcaaatgata aacttcctca aaaaactgta gaagaattga aaaggctaaa
    40981 tccatcaaaa gtagttgtga taggtggaaa caactcaatg ccaaattcag ttgttgaagc
    41041 tatcaaagct gtaaattcaa aaatatcagt acaaagaata ggtggagata caagatatca
    41101 aacttctatt aatatagcaa aagaaataga taagacgaat aatgtaagca agttgtatat
    41161 aggagcagga aatggagaag ctgattcatt atcaatagca tcactggctg gtaaagaaaa
    41221 aactccaata gtacttacac aaaaagatgg tgtagataat gaagctgaac aatttattaa
    41281 atcaaataag gtgtcaaata tatattttat tggtggagta gaaaaaatat ctaacaaggc
    41341 tattgaacaa gttggaaaaa tagcaaacaa agatatatca aacaataggg tggcaggtca
    41401 aactagacaa gagacgaatg ccaaagtaat agataaattc tactctcaat ctaagctaga
    41461 tggagttgta gttgcaaatc aagataaact tatagacgct ttagcagttg gaccattagc
    41521 agctaaaaac aattcaccag ttatacttgc tacaaatacg ttagataaat cacaagaatc
    41581 gtcattaaaa ggtaaaaatt catcaaagtt gtttgaagta ggtggaggaa tagcctcatc
    41641 tgtaatagac aagattaaga gtttaattga aaaataaata ttaagtaaat agaaaataag
    41701 aatgtattta gatataaagt tatatattta aatgcattct tattttattt tcattatctt
    41761 aacaatctaa taatattgag gttaaaatgt ataataattt gagctatatt aagtttaatt
    41821 ttaaataaag cagatatctt ttatatatat aaatataaat tcaattttga atatgtttaa
    41881 aaagaagtta aaaataattt tttaaattta ataaaataaa acactaatat atagacgaga
    41941 tatattcata tcatatatac tctataatat atggaaaata aagagaaaat aaatggaaaa
    42001 taatcacaat ataaatataa aatataaaaa tcatcagggg gatatgaaaa tgaacaaaaa
    42061 aatattatca ttaggtctag cagtatcatt aatcttagta aacttcaaaa gtgtaaatgc
    42121 atcatcagta gtagaaaaaa tatacggtaa agatagatat gaaacagcag caaagatagc
    42181 tgataaacag aattatgaaa cagtaatttt agtaaataca gaaaaatcac ttgcagatgg
    42241 attaagtgca agtggattat caggagctac aaaagctcca atactattta cacaacaaaa
    42301 taagatacca gcagacacaa atagatgtct aaaaaatatc aaaaaagcat atataatagg
    42361 tacagaagat actataagta aatcagtaga aaaagaacta gattctaaga atatagaagt
    42421 caaaagaatt ggtggagaag atagactaaa aacaagctat ttaatagcta aagaaatagc
    42481 aactataaaa aaagtggata aagtactatt aactaatgca tatagtggag aagcagatgc
    42541 aatgagtgta tcatcagtag ctactagaga tggagctcca atcatactta cagatggaaa
    42601 gagtgtgcct tttgatgtaa aaaatattca atcatattgt ataggttcag aggagataat
    42661 gagtaatctt ctagttaaaa atacaaactc agtaagaata gaaggaactg actgatttga
    42721 aactaacaaa aacgtaatag actacttttt taatagtgca gatggattct atgtatcaga
    42781 tggataccaa ttagtagatg caatagcagc cgcaccactt actaaaaact ctccaatggt
    42841 actagtaaat gatggaagcg ataaaactgt attagaagga gctaagaata taacctctgt
    42901 aggtgaaata gatgagaagg taatacaaca gtgtataaat gcttcaaatt caaatggaca
    42961 acccccaaca attacagttg ggagtacaga ggtatataag ggtgaaaagt ttgacactgg
    43021 caagttaaat atagtagcta aagataacac agggaaggta ttaccaatag aagttgatgg
    43081 gtttatagat acaaatagag taggtacata catattgaca ttaaaagcta cagacgaatg
    43141 gggaaaaagc acaggaaaaa gagtagaaat aaaagtgttg gatgacaaat ctcatgatta
    43201 caacagccca gaatttaaaa aaatggtatc tactgaaatg tataacttaa tcaattccta
    43261 tagaaaagaa aaaggcaaag agcctttggt agtgtctagt aggctagaag gtatggcaaa
    43321 tgcatggtct aagtatatga tggataaaaa agtatttgca cattatatag atggaaaaaa
    43381 tgctccacaa gtattttctg agtttggaat gagaagtgag gaaaatatag catatattta
    43441 tatagattct aagaacgttc aaactacaca agatgcaaaa gacttagcaa aggctatatt
    43501 tgaggtgtgg aagaagtctc cagagtacaa tgcaaacatg cttagtgatg agttctatag
    43561 tactggattt gggctttata tattatctga tggtcaagtg catgctactc aggagttttt
    43621 aaatggaaat gaaggtagct tgtaaaaaat tccataaatc ttgttttttt gttaatttac
    43681 tgatattata aaatgaaaat cacatctaaa gtactttttg taatctgttt tgttattgct
    43741 ttacaggtta tggtatttta ggtgtttttt gttttagtag tttatagatt ttgagggaat
    43801 cactactgag gatgtaattt atgattagct gaattatata aaaaaattag taggaggagt
    43861 ttgatgaagg taaataagag agtattatca ataggattga caatatcatt aataatggca
    43921 ggagtaccaa atatcaatgc actttcgagc atagaaaaga tacaagggaa agatagatat
    43981 gagacagctg ccaaaattgc acagaaacaa acatatgaaa atgtagtatt agtaaataca
    44041 gataatacac tagctgatgg attaagtgca agtggattag caggaacagt taaagcacct
    44101 atattattat cacaaagaaa cagtatacca tcagacacag aaaaaatgtt aaaagatgta
    44161 aagaaggttt atataatagg aactgaagat tcaataggaa aatcagttga aaatgaatta
    44221 aagcaaaaag gaatagaagt taaaagaata ggtggtaatg atagaattga aacaagttat
    44281 cttatagcaa aagaaatagc ttcaataaaa cctatagata aggtttttat aactaatggt
    44341 tatacaggtg aagcagatgc tatgagtgcg tcatcagtag catcaagaga tggagcacct
    44401 ataattttaa ctaatggtaa aaatgttcca tttgagaaaa aagaaggtgt tcaatgctat
    44461 gctcttggtt cagaggagat aataagtaat gatttagtta aaaaaactaa tgcagttaga
    44521 ttagctggag aagatagatt tgagactaat aaaaaagtta taaaacattt ttatagttca
    44581 gcaaaagaat tttatctttc taaaggttat caattagtag atgcagttgc gggttcttct
    44641 atagcaaaaa atgcacctat tgtattggtt gatggaaata gtgataagtc tgttcttaga
    44701 agtgctgata aaatcacagc actaggagga atagatgaaa agacactaga acaatgttta
    44761 agtgcatcat cactggatgc aagtgcacca acaataactg ttggaaattt aaatatttat
    44821 caaggtgata agtttgatat aagtaaactg aatatcgtag caaaggatag caatggtaat
    44881 gatttgacac cagagcttat aggaaatatt aatactgata aggttggtaa atacaaagtt
    44941 actataaaag ctactgatat tggtggaaag actacctcaa taagtgtaga agtaaatgta
    45001 cttgaatata agactaatga tatgaatagt agtgagttta aaagaatggt ttctagtgaa
    45061 atgtatagtt tggtaaattc atatagaaaa gaaaaaggga aagaagcatt acaagtttct
    45121 aaaaacttag aagggctttc taattcctgg tctaagtaca tggcagataa aggccagttt
    45181 tcacatgtaa tagatggtaa gaagtctgtt gaggtattta ctggatatgg gttgagaagt
    45241 gaagaaaata tagcttttgt accacttgtt acaaagagca cttatacgac aaaggatgct
    45301 agagaagtag caaatgtaat atttacagtt tggaaaaaat cggataaata taatgaaaat
    45361 atgctaaaca gtgattttgt atatactggg tttggattgt atatattgcc taatggagaa
    45421 gtgtatgcta ctcaagaatt tttaaataag taatttaaat aagtaaaatc aaatttttta
    45481 agttattaaa tattggagtt taagggtata aaaactcata tgcttgggaa aaataataat
    45541 ttcaaaaaaa tgacttgcaa aaagaataaa aatggattat tatagagatg tgagaaatat
    45601 taggaatata tggatgatta ttctatgtac ataataaaga gatgtaattt aatataatgt
    45661 tgggaggaat ttaagaaatg aataagaaaa atatagcaat agctatgtca ggtttaacag
    45721 tattagcttc ggctgcacca gtatttgcag caaatgaatt tgttgcggat tctaattata
    45781 ctgtagttca aggaaaatat caaaaggtta taactggttt acaagatgct ttgaaagatg
    45841 gtaaaatatc aaaaattgat gtaatatttg atggaagttc aattggagaa gtagagccag
    45901 gtggtgatcc tgcagctgca gctgttaaat tgaaaaattt agttgatgat aaattagata
    45961 atttaggcga tggaaaatat gttcaattta atgttgctta ttctactacg tctgtaataa
    46021 ctaaagcaga attaaagaat tattataaca aattagataa agaaaaaata cttgtgagta
    46081 atgaacctac aactacagta aatggactta ttaaagcaga ccaatcacct aatcaagcta
    46141 atggagtagc agcaaatgaa ccattaaaat tatcagatgt aataacactt tcttatgatg
    46201 atgaagctgg tacacttacg gcaactcaaa atgctactaa tgtagtttca aataaagtaa
    46261 aagacttaaa atttggtact gctactactg gttcatatac tgctggtaaa gaaataactg
    46321 ttccagctac tgcaacaata acagcaggaa caagtacaac agcggacttt aatcttgatg
    46381 atccatcaaa agcatttaaa tttgatccat caagcgctga aataagtgga tttgttactg
    46441 gtcaagctgg tcaaacttta aaagcaagta taaaaataat aaatgcaaaa gaagagtcta
    46501 tagatgtaga ttcaagttca cacagaacag ctgaagattt agctgaaaaa tatgtatttg
    46561 agccagctga tgttaatgca gcttatgagg cattaaatga tttatataaa gaaggattaa
    46621 caagtgacct tataactcaa gatggtggaa aatatcaagt tgttttattt gctcaaggaa
    46681 agagattaac tactaaagga gcaactggca ctttagcaga tgaaaattct cctcttaaag
    46741 taacaataaa agcagataaa gtaaaagatt taaaagatta tgttgaagat ttaaaaaatg
    46801 ctaataatgg atattcaaat tctgttgttg tagcaggtga agatagaata gaaacagcaa
    46861 tagagttaag tagcaaatac tataactctg atgatgacaa tgcaataact aaagatccag
    46921 ttaacaatgt tgttttagtt ggttctcaag ctgtagttga tgggcttgta gcttcacctt
    46981 tagcatctga aaaaagagct cctttactat taacttcagc aggaaaatta gattcaagtg
    47041 ttaaagctga gttgaaaaga gtaatggatt taaaatctac aacaggtgta aatacttcta
    47101 aaaaagttta cttagctggt ggagtaaact ctatatctaa agatgtagaa aatgaattaa
    47161 aagatatggg acttaaagtt acaagattat ctggagatga tagatatgaa acttctttag
    47221 ctatagctga tgaaataggt cttgataatg ataaagcttt tgtagttgga ggaacaggat
    47281 tagcggatgc tatgagtata gctccagttg cttctcaatt aagaaactca aatggagaac
    47341 ttgacttaaa aggtgatgca actccaatag tagttgttga tggaaaagct aaagatataa
    47401 attctgaagt aaaagatttc ttagatgatt cacaagttga tataataggt ggtgtaaata
    47461 gtgtttctaa agaagtaatg gaagcaatag atgatgctac tggaaaatca cctgagagat
    47521 atagtggaga agatagacaa gcaacaaatg ctaaagttat aaaagaagat gatttcttta
    47581 aaaatggaga agttacaaac ttctttgtag ctaaagatgg ttcaactaaa gaagatcaat
    47641 tagtagatgc tttagcaggt gctgcaattg ctggtaactt tggtgtaaca gtagataatg
    47701 aaggaaatcc tacagttgtt ggtaaaaaag cttctccagc gccaattgtt ttagcaacag
    47761 attctttatc ttctgatcaa aatgtagcta taagtaaagc tgtaaatgat gacgctaata
    47821 ctaagaatct agttcaagtt ggtaaaggta tagctacttc agttataagt aaaataaaag
    47881 atttattaga tatgtaatat aagttttaat aaaactttaa atataaaaag acttctcaga
    47941 tgagaagtct tttttgtgaa aaaaataaat acaaaataaa atagaggcta tttatagcct
    48001 ctattttatg tgagaaatac tcaaataaaa agatgtttaa tttatttaag taaagtcatt
    48061 atactataaa tcaaaataat tgtctactat tatggatttt taatgtataa tagtaattgg
    48121 acaatagaaa aggaggtact tatatgtcag ttatagatgc gatacttgac aaagcagatg
    48181 aacaagaaat aaagaaatta aatgttatag tagataaaat agatgcactt gaagagagta
    48241 tgaaaaaact atcagatgaa gaactaaaag acatgacagc tatatttaaa gatagactta
    48301 aaaaaggtga aactttagat gatatacttc cagaagcatt tgctgtagta agagaagtat
    48361 caaaaagaaa attaggaatg cgtcagtata gagtacaatt aattggtggt atagtaatac
    48421 atcaaggaaa aatagcagag atgaaaactg gtgaaggtaa aactctagtt gaagtagcac
    48481 cagtatattt aaatgccctt acaggtaagg gtgtacatgt aatcacagta aatgactatc
    48541 tggcagaacg tgataaggag cttatgcgtc cagtttatga atctcttgat atgacggtag
    48601 gagtaattat atctaaccaa gacccaagta taagaaaaga acaatataaa tgtgatataa
    48661 cttatggaac aaatagtgaa tttggatttg actatttaag agataatatg gtgccagatt
    48721 tatctcataa agtacaaaga gaactaaatt ttgctatagt agatgaagtt gactcaatat
    48781 taatagatga agctagaacc ccgcttatta ttgcaggaga tggtgatgaa gatttaaaac
    48841 tttatgagtt agcaaatagc tttgtaaaaa ctgttaaaga agaagacttt gagatggaca
    48901 gaaaagataa aactatagca ttaacagcaa gtggtataag taaagctgag tcattttttg
    48961 gtataacaaa ccttactgat ataaagaaca tagaattata tcatcatata aatcaagctt
    49021 taagaggtca caaacttatg gaaaaagatg ttgactatgt tatttcaaat ggagaagtaa
    49081 tgatagttga cgaatttaca ggaagagtaa tggatggtag aagatataca gatggacttc
    49141 accaagctat agaagccaaa gaaggtgttg agataaagaa tgaatctaaa actatggcta
    49201 ctgtgactta tcaaaatttc ttcagactat atgaaaaact ttctggtatg actggtactg
    49261 caaagacaga agaaggagaa tttgagtcaa tctataaatt aaatgttgtc caaataccaa
    49321 ctaataaacc agtgattaga gctgatttac atgataaggt atttaaaaca gaagaagaaa
    49381 agtatagtgc tgttgtagaa gaaataataa ggatacataa gactagacag ccaatacttg
    49441 taggaacagt ttctgttgaa aaatctgaga agctgtctaa aatgcttaaa aaacaaggta
    49501 ttaagcatca agtattaaat gctaaacaac atgataaaga ggcagagata atttctaaag
    49561 ctggtaaatt aaatgctata acgattgcta caaatatggc aggtagagga acagatattt
    49621 ctctaggtgc aggagataaa gaagaagaac aagaagttaa agatttaggt ggactttatg
    49681 ttataggaac agaaagacac gaatcaagaa gaattgataa tcagcttaga gggcgttctg
    49741 gtcgtcaagg agacccaggt acatcaagat tctttgtaag tcttgaagat gatgtaataa
    49801 agctttatgg tggaaaaact atagaaaaac ttatgaagag aacaagttca aaggaaaata
    49861 cggctattga aagtaaagca cttacaagag ctatagaaag agcgcaaaaa ggtgtagaag
    49921 gtaaaaattt tgaaataaga aaaaatgttc ttaaatatga tgatactatt aatgagcaaa
    49981 gaaaagttat atataatgaa agaaataaag tgttaaatga tgaagatata caagaagata
    50041 ttcaaaaaat ggttaaagac atcatacaag aagcaggaga aacttattta attggaagaa
    50101 aaagagatta ttatggatat tttaaacatt tatatagtac atttatgcca gcagatacat
    50161 tactaatacc tggtgtagat aaaaaaagtg tccaagagat aattgatagc acatatgaaa
    50221 tttcaaaaag agtttatgac ttgaaaaaga tgatgcttgg tattgataag gttgcagagt
    50281 tagaaaaaac agtactttta aaagtggttg atcaatactg gatagatcat atagatgcta
    50341 tggaacagtt aagacagtat ataggtctta aatcttatgc tcaaaaagac ccatttaagg
    50401 aatatgcttt agaaggatat gacatgtttg aagctttaaa taaaaatata agggaagcaa
    50461 cagtacaata cttatataaa tttaactaaa aaagttcatg taatttttat taaatgaaaa
    50521 ataaaatatt ttgattcttt atattgtaat tacaattaaa taattaaaaa atacaaatga
    50581 aaaagaagct gaaaatcagc ttctttttct gtgtgagaaa tacggaaaaa agagatgctt
    50641 gaaatttctt tcttccattt aatattatac tataaagaag atgaaaagtc tattagttat
    50701 aaactggatt attaaaaaaa attacaattt tgttactttt ttgtatacat aaatattaaa
    50761 aaaataaaat ataagaaaaa gtttatatct tttgtttaat tattacaata agtctcattt
    50821 attgaaataa tatcaaatat atattataat tggtaaataa ggaaaaaata ataaaaattt
    50881 gaatttttta gggggaaaat accatgaata aaaaaaatct ttctgtaatt atggctgctg
    50941 caatgataag tacatcagta gctccagttt ttgctgcaga aactacacag gtaaaaaaag
    51001 aaacaataac taagaaagaa gctacagaac tagtttcgaa agttagagat ttaatgtctc
    51061 aaaagtatac aggaggttct caagttgggc aaccaatata tgaaataaaa gttggtgaga
    51121 ctttatcaaa attaaaaata ataactaata tagatgaatt agagaaatta gtaaatgctt
    51181 tgggagaaaa taaagaactt attgtaacta taacagataa agggcatata acaaatagtg
    51241 caaatgaagt agttgcagaa gcaactgaaa aatatgaaaa ttcagcagac ctttccgctg
    51301 aagctaactc tataacagaa aaagctaaaa ctgaaaccaa tggaatttat aaagttgcag
    51361 atgtaaaagc ttcatatgat agtgctaaag ataagttagt tataacttta agagataaaa
    51421 cagaaacagt aacttctaaa actatatatg taggtattgg tgatgaaaaa gttgatttaa
    51481 cagcaaatcc agttgattca actggaacaa atttagaccc ttctgcagaa gtatttagag
    51541 taaataaaat cgataaacta ggtgtagcag gagctaaaaa tattgatgat gtccaattgg
    51601 ccgaaataac tataaaaaat agtgacctaa atacagtttc accacaagat ttatatgatg
    51661 gatatagatt aactgttaaa ggtaatatgg tagcaaatgg tacatcaaag tcaattagtg
    51721 atatttcagc aaaagattca gaaacaggaa aatataaatt tactattaaa tatactgatg
    51781 catctggaaa agcaacagag cttactgtag agagtattaa tgaaaaagat ttaaaagatg
    51841 ccaaagccgc attagaaggt aattcaaagg ttaaattgat agctggagat gatagatatg
    51901 caactgcagt ggctatagca aaacaaacaa aatatactga caatatagtt atagttaatt
    51961 caaataaact agttgatgga ttagcagcta caccacttgc tcaatctaaa aaagcaccta
    52021 tattattagc atctgataat gaaataccaa aagtaacttt agattatata aaagatataa
    52081 ttaagaaaag tccatcagct aaaatatata tagtaggtgg agaatcagca gtatcaaata
    52141 cagctaaaaa gcaattagaa tcagtaacta agaatattga aagactagct ggagatgata
    52201 gacatacgac ttctgtagca gtagcaaaag ctatgggttc ttttaaagat gcatttgtag
    52261 taggtgcgaa aggggaggct gatgctatga gtatagctgc caaagctgct gaacttaagg
    52321 ctcctataat agtaaatggt tggaatgatc tttcagcaga cgctatcaaa ttgatggatg
    52381 gaaaagagat tggtatagtt ggtggctcta acaatgtatc tagccaaatt gaaaatcaac
    52441 ttgctgatgt tgataaagat agaaaagttc aaagagttga aggagaaaca agacacgata
    52501 ctaatgctaa ggttatagaa acatattatg gaaaattaga taaactatat atagcaaaag
    52561 atggatatgg aaataatggt atgctagtag atgcattggc agcaggacct ctagcagcag
    52621 gtaaaggtcc aatacttcta gctaaaactg atataacaga ctcacaaaag aatgcactta
    52681 gtaaaaaatt aaatcttggt gcagaagtaa ctcaaatagg taatggagtt gaattgacag
    52741 taatacaaaa gatagctaaa atactaggtt ggtaataact tatactgtgg agaagattcc
    52801 ttaattaata caggaatctt tttctataat tatgctattt attgtaaatt ataacatttt
    52861 ataaagtagg aggatgtagt agtggtagat gcaactttat tttttacacc acatcaagat
    52921 gatgaaacct taagtatggg aagtgctata attgaacatg ttgaaaaaag tgatacacat
    52981 gtcatattat gcacagatgg tagtaaatct ataataagaa aagttttgga tgatggaggg
    53041 tcatgttctt atcatattaa agatgttcat aaatactctt tatcagaaag tgaattttca
    53101 aaagatagag atgaagaatt taaggatagc tgtgaagcaa tgggtgtaaa agaaagtaat
    53161 atacatatag aagataatag agcacatgat ggtgagttaa gtaaagaaaa ggcaaggaaa
    53221 ataatactta agtacttaga agaatatcca gatgcaaaag taaaaactgt tactccattt
    53281 aaagctagtg gaatacatga agaccataga gcattaggag aagctgcatt agagttatat
    53341 agagaaggca aaataaaaga tttaagattt tatgtagaac catacgacta taaggagttt
    53401 aaaaaagtta atccaaatgt agaagtatgg aaggtattac ctagtcaaga ggagaagtta
    53461 ttaagtgcaa tgaatgcata taaaaaatgg aatcctgaaa atggacatta tgctatagga
    53521 tatcattcag taaaatctca ttttgatgag ttagctacaa ataaaataca gtatgtacat
    53581 gctccataaa atattataaa agaattagga ggtaagaatt aatgaaaata tcaaaaaaga
    53641 tagtgtcttt gttaactatg acatttttaa ctgttacatt atatggaaat acatctaatg
    53701 catctacaaa agatacatta acgggttctg gaagatggga aacagcaata aaaataagtc
    53761 aagcaggatg gaaaaagtct gaaaacgctg tgttagtaaa tgataattca atagcagatg
    53821 ctttatcagc tactccattt gcaaaggcaa aagatgcacc aatattatta actcaaagta
    53881 ataaattgga tagtagaaca aaagcagaat taaaaagact tggtgtgaaa aatgtatatt
    53941 taataggtgg ttcaattgca ttaagttcag agattgaaaa gcagttaaat gcagaaaata
    54001 taaattttga aagaatatct ggaaatagta gatatgatac ttctttaaaa ttagctgaaa
    54061 agctagatag ggaaaaatct atatctaaaa tagtagtagt aaatggagaa aagggtcttg
    54121 ctgatgcagt gagtgttgga gctatagctg ctcaagaaaa catgccaata atactttctg
    54181 attcagagaa tggaactgaa gtagctgata attttataga tagtaaaggt atagaaaaat
    54241 cgtatgtaat aggtggtaca tattctattt ctaattctgt agaaagaagt ttgccaaatg
    54301 caacaagaat agcaggtagt agtagaagtg agacaaatgc aaaaattata gaagaatttt
    54361 acaaagatac tgatataaaa aatatttatg taacaaaaga tggtacaaag aataagaatg
    54421 atttaataga ttctttagca gtgggtgtat tagcttctaa aaatggctct ccaatacttt
    54481 tagcaggaaa caagctagat agttctcaaa aagatgtatt aaatactaaa attatagata
    54541 aagttactca aattggtgga ttgggtaatg aagatgttgt agataatata atagatatac
    54601 aagaaaaaac aaagtatact gttgaaacta tagaagaatt ttatgttgcg atgaaaaaag
    54661 ctgatgctaa tgatgttata atatttgaaa ctaaaaaaga tgatagtata aatgattctt
    54721 ttgaagtgaa aacaactaaa gcaataactg tagaatttga tggtatattt aaaaagacaa
    54781 taactataga tatgcctaat ggagatgtga ataactttgg tgaaatatct gatgatataa
    54841 gaataaataa tataagaaaa aatactttaa ttaatgaagg aagtatacaa ggtattgata
    54901 tttattctaa aaatggttgt aaaatagaaa ataaaagcga tggagatatt tggcttataa
    54961 caatagtatc agaagctaga ggtgtgtata tagtgaatga tggagatata tcaaaaattt
    55021 caaatagttg ttcagatgtc ataataaaaa actcaggaaa gattaattta gtgactggta
    55081 ctgaagaacc tgcgattagt ggaaaaaaac caataactaa tgatactgaa tatgatgatg
    55141 aaagagcgca tggattatct gttaaaacag aggcatgttc cacacctcaa aaaaattata
    55201 taatagtaac aatatcatct aagcctaaaa attcaaatta cgctatttat tatagagtag
    55261 tcggggataa accttcagct atgtatgtag gggaaaaaat taatcctaga gattggtatt
    55321 ctgtatcaaa atcagatgat tcatttattg aaaaagctaa gaatggatca tatatagaag
    55381 ttgttgaaat aaattcttca aataatagag tctctagatg gggaagatca agctcaactg
    55441 atgatggact ttaaagtgta aatatgattt taaaagtaga ttactatttc tagtagtcta
    55501 ctttttattt ttaaaaatta tcattttact aaaaaaataa tttaaaacta taacaactat
    55561 attagtaaat gcttttacaa tgatatcatt tacaaaaagt aaatcaatca ttacatacat
    55621 aattaacatt tcaacgacac cagaaagtag cctaaaacta acaaaagaca aaaattcctt
    55681 aaataagaac tttatttcaa ttcttttact ttcaaacaca aaaaacttat tagtaacata
    55741 agcaaataaa acagcaagta tccatgccaa agcatttgca accataaaat taaataaaat
    55801 aactctagta aaaaataaat acgaaactat atttacaaga gtagtaaatg ctccaaaaaa
    55861 taaatataat attgtctctc tatgtttttt taaaatcaaa tataatcctc ctaagctaat
    55921 ttaatataaa tatacttaaa gatactaaca aacattagta tagcacatct attaataaat
    55981 caatatatct taaaatatta taaaaaataa aactataata ttctataatt aatatgtact
    56041 cataatccat attttatcaa aataaaatat gaccttaatt tagaaatctg atataataaa
    56101 aattaaatac atataagggg gtaaacatga gaaagtataa atcaaaaaaa ttgtcaaagc
    56161 tactagcatt gttgacagtt tgctttttaa tagtgtcaac aatacctgtc tcagcagaaa
    56221 accataaaac tctagatgga gtagaaactg cagagtattc tgaaagctat cttcaatacc
    56281 tagaagatgt caaaaatgga gacacagcaa aatataatgg agtaatacct tttccacatg
    56341 aaatggaagg tacaacactt cgaaataagg gtagaagtag tcttccatca gcatataaat
    56401 caagtgtagc atacaaccca atggatcttg gtcttactac accagcaaaa aatcaaggaa
    56461 gtcttaatac atgctggtct ttttcaggta tgtcaacttt agaagtatat cttaaattaa
    56521 aaggatatgg aacgtatgac ttatcagaag aacatttaag atggtgggca actggtggaa
    56581 aatatggatg ggacttagat gatatgtcag gtagttcaaa tgtaacagcc ataggatatc
    56641 tgacagcatg ggcaggtcct aagttagaaa aggacatacc atataatctt aaatctgagg
    56701 cacaaggcgc aactaaacct tcaaatatgg atactgcacc tactcaattt aatgtaacag
    56761 atgttgttcg tcttaataaa gataaggaaa ctgtaaagaa tgctataatg caatatggtt
    56821 cagtgacatc aggttatgca cattattcaa catattttaa taaagatgaa acagcatata
    56881 actgtacaaa taagagagct ccattaaatc acgctgtagc gatagtagga tgggatgata
    56941 attattcaaa agataacttt gcatctgatg taaaaccaga atcaaatgga gcatggttag
    57001 taaaaagtag ttggggagag tttaattcta tgaaaggatt cttctggatt tcttatgaag
    57061 ataaaactct actaacagat acagataact atgcaatgaa atcagtatca aaaccagata
    57121 gtgataaaaa aatgtaccaa cttgaatatg ctggtcttag caagataatg tcaaataaag
    57181 taacagcagc aaatgtattt gattttagca gagattctga aaaacttgac tctgttatgt
    57241 ttgaaacaga ctctgtagga gcaaaatatg aagtatatta tgcaccagta gtaaatggag
    57301 ttcctcaaaa caattcaatg acaaaacttg caagtggaac agtatcatat tctggataca
    57361 taaatgtacc tactaattct tacagcttac caaaaggtaa aggagcaata gtagtagtta
    57421 tagacaacac agcaaatcct aatagagaaa aatcaacttt agcatatgaa actgatatag
    57481 atggatacta tctgtatgag gctaaagcaa acttaggtga aagctacata cttcaaaaca
    57541 ataagtttga ggatataaat acatatagtg aattttctcc ttgtaacttt gttataaaag
    57601 ctataacaaa aacatcttct ggacaagcta cttcaggaga atctttaact ggagcagata
    57661 gatatgaaac agcagttaaa gttagtcaaa aaggatggac ttcttcacaa aatgcagtat
    57721 tagtaaatgg agacgcaata gtagatgctt taacagctac accatttaca gcagcaatcg
    57781 actctccaat tcttttaaca ggaaaagata acttagattt aaaaactaag gcagagttac
    57841 aaagattagg aactaaaaaa gtttatctaa taggtggaga aaattcttta agcaagaatg
    57901 tacaaactca acttagtaat atgggtatat cagtagaaag aatttcaggt agtgatagat
    57961 acaagactag tatatctcta gctcaaaagt taaatagtat aaaatctgtt tcacaagttg
    58021 cagtggcaaa tggtgtaaat ggacttgcag atgcaataag tgttggtgca gcagctgctg
    58081 ataacaatat gccaataata cttactaatg aaaaaagtga gttgcaaggt gctgatgagt
    58141 ttttaaattc atcaaaaata actaagtctt atataattgg tggaacagct actttatcat
    58201 caaatttaga aagtaagctt tcaaatccaa caagacttgc aggaagtaat agaaatgaaa
    58261 ctaatgctaa gataatagat aaattctacc ctagttcaga tttgaaatat gcttttgttg
    58321 ttaaagatgg ttcaaagagt caaggagact tgatagatgg attagcagta ggagcattag
    58381 gtgctaaaac tgattcgcca gtagttctag ttggaaataa gttagatgaa agtcaaaaaa
    58441 atgtacttaa gtctaagaaa atagaaactc ctattagagt tggtggaaat ggaaatgaga
    58501 gtgcttttaa tgaactaaat actcttttag gaaaatagtg aatggaaaga tagtaaggat
    58561 atacatttta ttattaaaaa atgttcaata gaagtttata aatataaaat aacaaaaagg
    58621 ggtgtctcaa aatgaacttt ttagttcatg aggcactctt ttttgttgtt ttatattttt
    58681 atcatttaaa taatgaaaaa taggattgtc tatattttta gatagttttc ttattattga
    58741 tgaaaaatgt aataatttta aactgtaaaa tagtgtaaag ttattttata atataaatat
    58801 agtaagattt taaacaaaat aagggggaat aataatgaaa gcaccaaaaa ctattttaac
    58861 aatactaaca atagcgctta ctttaagtag catttctata ataccatcat atgcacttac
    58921 agaggaaaaa ttaataggta atggtagata tgaaactgct gtaaagataa gtcaaaaggc
    58981 atacagttct agtcctaatg tagtgctagt taatgacaat tcattatctg atgcactttc
    59041 agctacacca tttgcaaagg ctaaaggagc accaatactt ttgacagaaa gtgataagct
    59101 agatgatagg actgaaaaag agataaaacg tctaggagct aaagatattt atttgatagg
    59161 aggtactgct gtacttaata aagatataga gaacaagtta aaaggtaatg gacttaatgt
    59221 agaaagaatt aatggtaagg atagatatga aacttcactg attttggcta ataagcttaa
    59281 ggatataaaa gatattaagg aagttgctgt agtaaatggt gaaaaaggat taagtgatgc
    59341 tgttagtgtt ggagcaccag cagctcaaaa taagatgcct ataattttgt ctagtcctaa
    59401 ggacggagta gaagcatttg ataaatttat aagggatgag aaagttataa aagcatatgt
    59461 aattggaggt acaacttcag tttcaagagc tgtagaaaag agtcttccta atgcagaaag
    59521 agtgagtggg aaagatagaa atgagactaa tgctaaggtc atagaaaagt tttacactga
    59581 tacaaattta agtaatttat atgtaactaa ggatggtagt aaaaatgaga atcaactaat
    59641 agactcgtta gctgtcggag tattggctgc aaaaaatgag tctcctgttg ttttggttgg
    59701 taataaatta aacacaaaac aaagagatat acttagtact aaaaaattaa atactataac
    59761 acaggtaggt ggaaatggaa atgaagaagc ttttgatgaa attaaaagtc tacaagaaaa
    59821 aactgtgttt gaagcaaaaa ctgttgaaga attaactgat atgataaata tagcaagtcc
    59881 taatgatatt ataaacttta aaccaaaaga gaatactgta aatgaagctt ttagaatggt
    59941 aacaaataaa ccaataactg tgaatataaa gggtgattgt tcaaagactc ttacagtaga
    60001 tatgcctaat ggtgaagtga ataactatgc tactttggta aatgttatag ttagaaatat
    60061 tggagaaggt ggatttaata atcatgatac aatcactata ttgtcagttc gtgacaaaaa
    60121 tggtagagtt atagaaaata ctagaaattc agatatagat acgttgatga tattagcttc
    60181 tgctaatgat acaaaattga ttaatgatgg atatatagga aaacttatag ataattcatc
    60241 taatagtgat ataactaata atggtactat agataaaaaa gttaatcagg ttgaagattt
    60301 agaggctaaa gtagattcaa ttgaaaaagc tatagattct ataagtcaaa aagtcaataa
    60361 aatacaagat atactagata agcttggatt tctaaagaaa tttcttagct aagaatatat
    60421 atatttaagt gaagtgtgtg ttgtaattgt tctaaattta tgtagttaaa caggtttata
    60481 tacaaataga tttatagata aatatgtagt ttagtatata tttgaataag ctttggggag
    60541 gtaacatggg gattggaatt gaatgtaaca aaaaaataaa taaaagtatt gacaaaacta
    60601 gaataaagaa aaataatct
//
LOCUS       DAEMHZ010000007        55139 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000007 DAEMHZ010000000
VERSION     DAEMHZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.007"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..1570
                     /locus_tag="KPK72_000399"
     CDS             23..1570
                     /locus_tag="KPK72_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2487645.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1573..1971
                     /locus_tag="KPK72_000400"
     CDS             1573..1971
                     /locus_tag="KPK72_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2487646.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2011..3744
                     /locus_tag="KPK72_000401"
     CDS             2011..3744
                     /locus_tag="KPK72_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG2487647.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKNGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3976..5187
                     /locus_tag="KPK72_000402"
     CDS             3976..5187
                     /locus_tag="KPK72_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2487648.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5380..5640
                     /locus_tag="KPK72_000403"
     CDS             5380..5640
                     /locus_tag="KPK72_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG2487649.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            5993..7705
                     /gene="ptsP"
                     /locus_tag="KPK72_000404"
     CDS             5993..7705
                     /gene="ptsP"
                     /locus_tag="KPK72_000404"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG2487650.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            8028..9577
                     /locus_tag="KPK72_000405"
                     /pseudo
     CDS             8028..9577
                     /locus_tag="KPK72_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            9603..12305
                     /locus_tag="KPK72_000406"
     CDS             9603..12305
                     /locus_tag="KPK72_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2487651.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNAYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(12811..13476)
                     /locus_tag="KPK72_000407"
     CDS             complement(12811..13476)
                     /locus_tag="KPK72_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487652.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            13820..15250
                     /locus_tag="KPK72_000408"
     CDS             13820..15250
                     /locus_tag="KPK72_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2487653.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEETEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            15618..16196
                     /locus_tag="KPK72_000409"
     CDS             15618..16196
                     /locus_tag="KPK72_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2487654.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCLIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            16226..16372
                     /locus_tag="KPK72_000410"
     CDS             16226..16372
                     /locus_tag="KPK72_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2487655.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            16677..18056
                     /gene="scfB"
                     /locus_tag="KPK72_000411"
     CDS             16677..18056
                     /gene="scfB"
                     /locus_tag="KPK72_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG2487656.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            18085..18582
                     /locus_tag="KPK72_000412"
     CDS             18085..18582
                     /locus_tag="KPK72_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG2487657.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            18880..20544
                     /locus_tag="KPK72_000413"
     CDS             18880..20544
                     /locus_tag="KPK72_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG2487658.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            20557..23007
                     /gene="recJ"
                     /locus_tag="KPK72_000414"
     CDS             20557..23007
                     /gene="recJ"
                     /locus_tag="KPK72_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG2487659.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            23020..23532
                     /locus_tag="KPK72_000415"
     CDS             23020..23532
                     /locus_tag="KPK72_000415"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG2487660.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            23606..25813
                     /locus_tag="KPK72_000416"
     CDS             23606..25813
                     /locus_tag="KPK72_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG2487661.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            25838..26287
                     /locus_tag="KPK72_000417"
     CDS             25838..26287
                     /locus_tag="KPK72_000417"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG2487662.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            26302..26916
                     /locus_tag="KPK72_000418"
     CDS             26302..26916
                     /locus_tag="KPK72_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2487663.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            27007..28563
                     /locus_tag="KPK72_000419"
     CDS             27007..28563
                     /locus_tag="KPK72_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG2487664.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            28553..29815
                     /locus_tag="KPK72_000420"
     CDS             28553..29815
                     /locus_tag="KPK72_000420"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG2487665.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            29889..31676
                     /gene="aspS"
                     /locus_tag="KPK72_000421"
     CDS             29889..31676
                     /gene="aspS"
                     /locus_tag="KPK72_000421"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG2487666.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            32135..33367
                     /locus_tag="KPK72_000422"
     CDS             32135..33367
                     /locus_tag="KPK72_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2487667.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            33381..34187
                     /locus_tag="KPK72_000423"
     CDS             33381..34187
                     /locus_tag="KPK72_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG2487668.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYMISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(34299..35168)
                     /locus_tag="KPK72_000424"
     CDS             complement(34299..35168)
                     /locus_tag="KPK72_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2487669.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            35540..36967
                     /locus_tag="KPK72_000425"
     CDS             35540..36967
                     /locus_tag="KPK72_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG2487670.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(37044..37313)
                     /locus_tag="KPK72_000426"
     CDS             complement(37044..37313)
                     /locus_tag="KPK72_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487671.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(37316..38758)
                     /gene="nhaC"
                     /locus_tag="KPK72_000427"
     CDS             complement(37316..38758)
                     /gene="nhaC"
                     /locus_tag="KPK72_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2487672.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(38783..39949)
                     /locus_tag="KPK72_000428"
     CDS             complement(38783..39949)
                     /locus_tag="KPK72_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2487673.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNTIN"
     gene            complement(40174..42294)
                     /locus_tag="KPK72_000429"
     CDS             complement(40174..42294)
                     /locus_tag="KPK72_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2487674.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            42522..42923
                     /locus_tag="KPK72_000430"
     CDS             42522..42923
                     /locus_tag="KPK72_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487675.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            42966..43259
                     /locus_tag="KPK72_000431"
     CDS             42966..43259
                     /locus_tag="KPK72_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG2487676.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            43274..43714
                     /locus_tag="KPK72_000432"
     CDS             43274..43714
                     /locus_tag="KPK72_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG2487677.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSSESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(43806..44279)
                     /locus_tag="KPK72_000433"
     CDS             complement(43806..44279)
                     /locus_tag="KPK72_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2487678.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(44279..45067)
                     /locus_tag="KPK72_000434"
     CDS             complement(44279..45067)
                     /locus_tag="KPK72_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2487679.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            45395..46639
                     /locus_tag="KPK72_000435"
     CDS             45395..46639
                     /locus_tag="KPK72_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2487680.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            46962..48059
                     /locus_tag="KPK72_000436"
     CDS             46962..48059
                     /locus_tag="KPK72_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2487681.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILDKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            48050..48748
                     /locus_tag="KPK72_000437"
     CDS             48050..48748
                     /locus_tag="KPK72_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2487682.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIINRMLKENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            48751..49815
                     /locus_tag="KPK72_000438"
     CDS             48751..49815
                     /locus_tag="KPK72_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2487683.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            49998..50702
                     /locus_tag="KPK72_000439"
     CDS             49998..50702
                     /locus_tag="KPK72_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2487684.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            50695..52017
                     /locus_tag="KPK72_000440"
     CDS             50695..52017
                     /locus_tag="KPK72_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2487685.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            52183..53556
                     /locus_tag="KPK72_000441"
     CDS             52183..53556
                     /locus_tag="KPK72_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487686.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(53928..54866)
                     /gene="pdaA"
                     /locus_tag="KPK72_000442"
     CDS             complement(53928..54866)
                     /gene="pdaA"
                     /locus_tag="KPK72_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2487687.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        21481 a         5960 c         9357 g        18341 t
ORIGIN      
        1 tttaaaaaat aggggaattt atatgaataa aaatagcatg acaagaattt acagtttaaa
       61 taaaataatt tttattataa ttggaatttt aggaatagtt ttactcacaa gacatattga
      121 ggtagatatt aaagttttaa ttttaatttt attaaccttg tttagtataa atgtatttat
      181 ttcatatctt aaaattaagt tgtatgagga taaaattaat ttaggattga taaataattc
      241 agataagttt ataaatatta ctaaagatga gaacttccta aaacatgttc aaaattatat
      301 tatcagtaat gaaaaagaaa actgtgtaat ggcttgtttt gatttatgta gactcaaact
      361 tataaatgat atatatggtt atgagctagg ggatgaagtg ttaaataata ttttaagtaa
      421 cttaaaaaat tattttggaa aagaagctat ttatggaaag ttaaaaagtg atgtttttgt
      481 attaataata aagcttgaaa atagggaaca gaagattcct tatttagtaa atcttattaa
      541 gaataaaatt gtgatactaa gtcagattga cagttttaaa atggatataa atattgaggc
      601 ttctattgga atttataaat ttaacaataa tgaaattgat ataaaaaaag ctatagataa
      661 tgctgatatg gcaaggctta aatcaaaagg actaaagcac atagagtatg tagaatttga
      721 taatgctatg gaagaagaaa tacaatcaat tatgaaaata gagagagatt tattcttggc
      781 tattaaaaat aaagagtttg taattcacta tcaacctaaa gttgattcaa gtacaggtaa
      841 cattataggt tcagaggcat tgataagatg gttgcaccca tcattaggca tggttggacc
      901 aaataagttt atacctatag ctgaaaaaaa tggtctgata aacaatattg gtagatggct
      961 aattcaagaa gtttttatta ccattaacaa gtggatttca gaaggtataa atgtcttacc
     1021 agtctcaata aatctttcta gagtagaact atataaaaat gatttagttg atttttttaa
     1081 actgatgttt aatacatata atattccaaa agaacttata gaattagaaa ttacagaaac
     1141 gacagcacta agagatgtaa aatttataag tgaaaggctt tatgagatta aatctttagg
     1201 tatgaatata tcattagatg attttggagt aggtaattca aattttatca accttaaggg
     1261 gataccacta gatattataa aaattgatag gtcacttata ttggatatag ttacaaatac
     1321 aaaaacaaag tttatggtga aagcaattgt taacctttca catgatttaa acgtaactgt
     1381 tatatgtgaa ggggtagagg atatgcatca ggtcaaagtc ttaagtgaac tgggatgtaa
     1441 tttcatacaa ggttatgtat tttttaaacc tttggatgag atgaattata aaaaacttct
     1501 tactgacgga agttatataa atctgaaaga tactctttta gataaatgca tgacagttga
     1561 ggaggagtaa ggatgatttc agaagatgta gattattcaa taagaatcat aagagctata
     1621 tttaataatg gagaagataa accaatagat gctaaaatga tattaaaaag tgaagatata
     1681 ccaatactaa cactatacag actgcttaga aagctttgtg caaacaatat tcttactttt
     1741 aacaaagaag atggcaagac atacatgtta aatgtgtcat tggatacaat aagtctaaga
     1801 actattgtag aagtaattga tggaaaaata tatataaata aatgtacgag atatgtatct
     1861 aattgcagta attgcttaaa aggttgtaaa gtaaatcgtg aattgctaga aatagaaaaa
     1921 gaaatttata aacttttatc gagaaaatct ataagacaaa tactagaata aaaaattttc
     1981 agctatgata ataattggag gttttcggtt atggatgata tatctcaaga taattttcta
     2041 ctgtctaaag agtatgagaa tagcttagat gtagatacta aaaaagcaag tggaatatat
     2101 tatactccta aaataattgt agattatatt gtaaaaaaaa ctttaaaaaa tcacgatata
     2161 attaaaaatc catatccacg aatacttgat atttcctgtg ggtgtggaaa ttttctttta
     2221 gaagtatatg atattttata tgacttattt gaggaaaata tatatgaatt gaaaaagaag
     2281 tatgatgaaa attattggac tgttgataac atacaccgtc atatattaaa ctattgtata
     2341 tatggtgctg atattgatga aaaagctata agtatattaa aagatagctt aacaaataaa
     2401 aaggtagtaa atgatttaga tgaatctgat ataaagatta atctattttg ttgtgatagt
     2461 cttaaaaaga aatggcgata taagtttgat tacatagtgg gtaatccacc ctatattggt
     2521 cataaaaaac ttgaaaagaa atataaaaaa tttctcctag aaaaatatag tgaagtttat
     2581 aaggataaag ctgacttata tttttgtttt tataaaaaga ttatagatat tttaaagcaa
     2641 ggagggattg gcagtgttat aactccaaga tattttttag aaagtctatc aggtaaagat
     2701 ttaagagaat atattaaatc aaatgtcaat gttcaagaaa tagtggattt tttaggtgca
     2761 aatatattta aaaatatagg ggtatctagt tgtattctaa cttttgataa gaaaaaaaca
     2821 aaagagacat atatagatgt atttaaaatc aaaaatgagg atatatgtat aaataaattt
     2881 gaaacattgg aagaacttct aaagagtagc aaatttgaac attttaatat aaatcagaga
     2941 ttgctatctg acgaatggat acttgtaaat aaagatgatg aaacttttta taataagata
     3001 caagaaaaat gtaaatatag cttggaagat atagcaataa gttttcaagg aataattaca
     3061 gggtgtgata aagcgtttat tttaagtaaa gatgatgtaa agttgaacct tgtagatgat
     3121 aaatttttaa aatgttggat aaaaagtaag aatataaata aatatatagt agataaaagt
     3181 gaatataggc taatatactc aaatgatata gataatgaga atacaaataa aaggatacta
     3241 gatgaaatta tagggctata taaaacaaaa ttagaaaaca gaagagaatg taaaaatgga
     3301 ataaggaaat ggtatgaact tcaatggggt agagaaaagc ttttctttga aagaaaaaag
     3361 ataatgtatc cttataaatc aaatgaaaat agatttgcta tagattatga taataatttt
     3421 tcaagtgcag acgtatattc tttttttata aaagaagagt atttagataa attttcatat
     3481 gaatatctag ttggaatttt aaattccagt gtttatgata aatactttaa aataactgct
     3541 aaaaaaatga gtaaaaatat atatgattac tatcctaata aggttatgaa gataaggatt
     3601 tttagggata ataattatga agagatagag aatttatcaa agcaaataat atcaatttta
     3661 ttaaataagt ctatagataa aggaaaagta gaaaaattgc agataaaaat ggataacttg
     3721 ataatggatt ctttaggtat ataagcagtt gtaagtatta aaaaattata atcttttaat
     3781 tgtttattta aatttagaaa taaaaagtta gccatataaa aaatattaaa aatatatact
     3841 tttcaatatt tttttgataa aataagaatg aatttgtgca attttatttt tctccaataa
     3901 ctaaaaatag ttttttgaat taatatttgc ttaagaataa aaatcagaaa cgtttttgat
     3961 atagtaggtg ataatatgaa aggattagtt ttagaaggtg gaggaacaaa aggagcctac
     4021 caaataggtg catataaagc tttaagagag cttggtatag agtttcaagg agtaactggc
     4081 acatcaattg gtgctttaaa tggagcatat ataattcaaa atgatattga agtaatggaa
     4141 gatatttggt taaattatga ctatactcat tttatgaata ttgatagaga aacctatgag
     4201 aggtttaaaa atgtagattt cactactaaa aatctcaatg cagtgtttga gcttataaat
     4261 aaagctagaa aaaatgaagg tatagatata agcccattaa gacaactaat ggagacaaca
     4321 ttaaatgaag acataataag gaaatcaaaa aaagactttg gacttgttac ggtctggtgg
     4381 gataaaaaaa ttaatccata tccattgtat atcgaagata tacctaaagg aagattgatt
     4441 gattatttaa ttgctagttc cagtttgcct atttttcaaa cgaacatgat ggatgataag
     4501 ttgtatcttg atggaatgtt ttgtgataac atgccaatag gactcttgga agacaaaggt
     4561 tatgaagatg ttgttgtaat taggttgatt gatgatttta ttgggaaaat gaatctaaat
     4621 cgacatcaaa atataaatat aaaaactata ataccatcag aatatctagg aggttctctt
     4681 aataaagata gagataatgt agaaaaaaac atccgactag gttacttaga tactatgaaa
     4741 gcatatgaaa gatgcactgg aattaagtat tattttgatg tagagtataa atataatgaa
     4801 gaatattgtt ttgataaatt tagaagtatg agcaatgaaa caatacaata tatctgtaat
     4861 ttattgaata taaaaagaga tgcttcactt agaactgtta tggaaagtgt aataccaaag
     4921 cttggtgagg tgcttggatt gccaaaggat ttttcatata aagatttgtt ttactgtata
     4981 tatgaaaaga aacttgaaga aaataatata aacaggataa aattatatga ttttaacaag
     5041 gttatagatg ttgtaaatac aaatattgat tctaatagaa atttagcttc taattttgaa
     5101 gttagaccta aaattagtat taaaaagaac aaaaacacaa aattattgac aaattgtatt
     5161 ataaaagaca taaaaaatca aagttaaata aaaaacaaaa aaaaatttgt aattatactc
     5221 gaaaaaagtg tatattagta gtataatatt tttgtagcac tagtgggaac aaaaaatgac
     5281 cataggacaa aaaagtacca cttagtacca ttaaagtaaa aaacaggaaa acaattttaa
     5341 aaaatataaa atgtaaagtt tataggagga tttaaaaaaa tggaaaaagt agttagtatc
     5401 aaaaatgcaa gtggattaca tgctagacca gcaggaatgt ttgttaagaa ggcagctgaa
     5461 tttaaatcaa cagtagaggt tgtagcaaaa gacaaaactg taaatgcaaa atcaataatg
     5521 ggaataatga gtttaggttt agctcaagga gaagaattaa agttagttgt aaatggagaa
     5581 gatgaagaag tagctttagc tgcaatggtt gaattagttg aaagtggatt tggagaatag
     5641 atataataat ttttaaaaat ttgatattta gaatctaata ttacttaaag taaagtaaca
     5701 gagaagtatt aaacatatac agaccaacat ttgtagatat ttaatttctt tgttactttc
     5761 atgtttattt aatcagaaga ttaagactat tgttaattgg gtataaaaat ataaatattt
     5821 tagaaaatga aataagatat ttttaatcat aaattcagag taaaaataca gtaacaaata
     5881 gtttataaaa ttttataaaa aattataagt aaataaaact ataaaaatgt aaattattta
     5941 tataaattta taaagtttat gtgctagatg atatttttta ggaggtaggg ttatggctta
     6001 caaaggaata ggtgcttctc ctggtgttgc tttagggaaa gcgttagtag ttgaacatag
     6061 tgaattagta attgaaaaaa aatctataga taatgttgaa gctgaaattg ctaaattaga
     6121 agatgcagtt gcagtttcta aagaagaatt agtaaaagtt aaggaaaagg cttctgaaga
     6181 attaggagca gaggaagcag aaatatttga agcacattta ttagttttag aagacccaga
     6241 gttaattggt tctgcaatag ataaaattaa aactgaaagt gtaaatgcag agtatgcttt
     6301 aaatgaaata aaagagatgt ttgttagcat gtttgagtct atggacaatg agtacatgaa
     6361 agaaagagcg gcagatataa aagatgttac aaatagaata cttagacata tattaggaat
     6421 taaagttgtt gatttatctg ctttaagcga agaagttgta ttaatagcac atgatttaac
     6481 tccatcagac acagcaacta tgaataaaaa aatggtttta ggatttttaa ctgatatagg
     6541 tggaagaact tctcatactg ctatcatgtc tagaacttta gaaatagctg caatcgttgg
     6601 attaaatgat ataacttcaa aagttaagga tggagatttt gtggttttca atggagacac
     6661 aggtgaagta atcgtgaatc ctgatgaaga gactataaat aaatatactg aattaaaagc
     6721 taagtatgaa gatgagagaa aagcattaca gctattaaaa ggaaaaccat ctgtaacttt
     6781 agacggaaaa catgtggaat tagctggtaa cataggaaca cctaacgata tagaaggtct
     6841 aataaagaat gatgctgaag gtgttggtct ttatagaaca gaatttttat acatggatag
     6901 agattctttc ccaacagaag aaatacaata tgaagcatac aaagctgtat tagaaggaat
     6961 ggatggaaag cctatagtta taagaacatt ggatataggt ggagataaag aattaagtta
     7021 tttaagtatg gaacctgaaa tgaatccatt cttaggttac agagcaataa gactttgcct
     7081 agatagaaaa gatatattta agactcaatt gagagcttta tatagagcta gtgttcatgg
     7141 aagattaaga atcatgttcc caatgatttc atctttagaa gagttgttac aagctaaaga
     7201 agttgtaaaa gaagttttag cagaacttga ttctgaaggt gtggcttatg ctaaagatgt
     7261 agaaattggt atgatgatag aagttccatc tgctgctgtg atatctgatg tattggctaa
     7321 acacgtagat ttcttctcta taggaactaa tgatttaatt caatacactt gtgcagttga
     7381 tagaatgaat caaaagataa gttatcttta caatcaattt aatccagctg ttttaagact
     7441 tataaagact gttatagaca atgcacataa agaaggcaaa tgggcaggta tgtgtggaga
     7501 atctgctgga gaccaaaaaa tgataccaat acttttaggt atgggattag acgagttctc
     7561 tatgtctcca atatcaatac ttcctgctag aaagttaata acttcagtta aagagtcaga
     7621 tatgaaaaaa ttagctgatg atgtacttaa tatgggtact gctgaagaaa taaaaagcta
     7681 tatagaaaag acttttaata tataattaat gttatatagt tactaaaatt catttttcta
     7741 aataacaaaa ttatatttta cgaataaaaa aactatagta tatcatatat actatagttt
     7801 ttttgttata taaattacgt atcaaaattt gtaaaatgaa tagatataat tactaaatat
     7861 gtgatagtat tggctataat aggatataaa aaataatatt gtaataaaaa gtttatattt
     7921 agtaatgaaa atttaacgtt ttacttgaaa ttatgtgttt tgtaatgtat tattaaggtt
     7981 ctaataggaa cattttcatc aatttttcta tagtgggggt aggaattatg aggtacaaaa
     8041 aaagaaatat agataagtca agtgaacttt catttcaatc tctagcaaaa tattttataa
     8101 ctttctatat agtaattata ttaattattc tttctatgtt ttgtataaat acaatatggg
     8161 ttatgaaaaa agataatcaa gatttattat ctacaacgtc aaatcgtatt gaagataggg
     8221 taggagaaac aattaagcta ttgaactctt tgagtaaaga taattacata atagataata
     8281 gtgtgaatgg catagataaa agtaaagaac tttcaatgta tagtgatgaa tttgggtata
     8341 tgatgatatg ttttgttgat aagaatataa ttgtatggga tgaagaaggt ggtaatatta
     8401 gtctagctgg tcgtgattat atgcagaaac tttttgcaac aaaaaaatat caagttacag
     8461 atagttttgc agcaggggct gatggaaaaa ctttaaacta cactattgca tcacctataa
     8521 taaagaatag aaatgtagat ggttgtgtat ttgcagcaat ttactttgat gagataaagg
     8581 atatactcaa acaaggaaaa ggtcaacagc aacagaattt catattattt ggaagtgaaa
     8641 atcaaataat gaatttaaca agtgatgaaa ctgcatatgg agagaagtat atgaagttat
     8701 cgcagaaaac aaaatatctt ggaaccaatg caaaaaagta gaaaaggata tgtttaataa
     8761 taaatcaggt gattattggg cttttgaaaa tggaaaatta tactttatgt cctataaacc
     8821 aattaagaac acaaattgga gtttattttg taaagttaat ttcttagata tttttgcatt
     8881 aaatttaatt cctatgggag ctattctatc tgttataact ttagtattta ttattttgat
     8941 ttgtatggag aaaagcttca tgaaaaaacg aatggaagtt attgatatgc ttttaggttc
     9001 tgttcaagaa ttagaaaaac gtatatatca aacagaaaga cctagtgaag tagactttaa
     9061 agaaattatt cagctcacaa gtaaaggtct gactgatgga ttaactggtg ttgtaacacg
     9121 tactgtattt tttgaccaaa tttataatcg tgttgaaatg gaggaaaatg ataaattatc
     9181 tgtaatttca tttgttgatt tagatgattt gaaatgtatt aacgacttat atggacatga
     9241 aattggggat atggttttga aaaatgttgg atatgtactt cgtgaatatg agataaaata
     9301 taatggttta attgggcgct atggtggaga tgaatttgtt atgttgttat ctggttttac
     9361 atcgagagaa gaggtggagg atttattgga tgagattgct attcgcttac atactgaagt
     9421 actaacagat gatggtatca ttcaaactca ttgtagtgtt ggagctgtag tatgtagtgt
     9481 aaaagacaat aaagatatag ataatttatt aaaacaggct gatgaagctt tatactatgt
     9541 taaacgtaat ggaaaaggta attatcattt agtttaatgt atgtggaagg agtttatatc
     9601 atatgaaaaa tgtatctaaa aaattttttg tatgtactat tattgtatta ataacatttt
     9661 tatgttttag taaatttgct atttatgttt ttgacaaaca agaaaagaaa gaaacaaaag
     9721 tagtagaaaa catgttaaat gcctacaaga ataacattat tttaaatctg actgaaaaat
     9781 ttaattatag caaagacttg acatcagttt tagtttcaaa caattattct atagataaat
     9841 taaaacctat tgcagatgaa attcttaaaa acaattcttg catatatatt tcagttgtag
     9901 aaaaatataa tgtagctgca tttttttcta attctgtcaa aaaagatatt atcaatactc
     9961 caatacagga actgaattat tcttattcat tagcaaattc tatcaaagga ttagtcgtag
    10021 aaggtccttc taaagaccag ttttcaaatg gagatgtgtt tttaattact aatcctattt
    10081 ataaaaatgg agagtactgg ggagaaatta cagtagcttt agatgccaaa aaagttgtag
    10141 attcttttaa cttaaataaa ttaaaagaat ctggatttga gtatgaactt tggcgtgtac
    10201 acctagataa tggttcaaaa tctacaatag aaatttctac taaagatttt aatttttcca
    10261 aatcacatga aattaagttt aaacttccga ctgtttggac tcttagcatt gtgtacaaaa
    10321 atggatggaa taataagaat atagatttta ttgtatttgt tatattgtta attatttcca
    10381 tattaatagg tgtgatgaca tttatttaca ttgaaaagaa gtcgattagt aaggaattaa
    10441 atcaacagag tcttaaaaat ttagaaactg gtattttgaa tagagaaggt tttttaatta
    10501 agttacaaga gcatctgaat agaaaaaata atgaaatagt gttgctgtat atatgtatta
    10561 ctaattatag taaatttgct ccagttatga gctttgaaaa aagacaagat tatcttacac
    10621 atatagtaaa aaatctagat aattacataa aaagttctca ttttattgct cgtataagtg
    10681 aggaaggttt tgttctagct ttagaagaaa attgtgataa aaatgaattg cttaacattg
    10741 aaaaagggat ttccttagag cttttacgaa aaattaaagt agaaaataaa caagtatttt
    10801 taacgattgg ttatggctca tctaggtgtg ttgatgatag aaaagatgca gaatctctgt
    10861 tactttcagc tattgaagat tatgagagaa gatttccagc aatgtctcct attcatgatt
    10921 ttactaaaaa gtgtgaagat atgcttgatg aaaagaaaca gattgtattt ggtgaatatc
    10981 cagatttaga aataaacaag ttgtctaaag taatatacaa atattataaa aaagtacagc
    11041 gtattatgta taaagattat aatattgata ttggaaatag acgagggtat atgcgtgatg
    11101 tcgaagtatt gattgattat aattctgaga gaatgttata cttattttta atagacatat
    11161 gcaagtttgg aaaatttaat gaactgtttt ctattgaaat aggagatgtt attttaaaag
    11221 aagttagtaa caaattaaaa acaatttttt ctaattatct atatcgtatt aatggagatg
    11281 tatttttagg catttcaata gataaagaat cttacttaga tactttacat aaggttaatg
    11341 aagtatttaa tagttcttta agtgtagaag atgtactttt tgatgtaaaa tgtaatattg
    11401 gtatttgtca atatccatat catggtgaaa atccatatga ccttctggaa aaggtacaaa
    11461 ttgcattgga gtatgcaaaa aaggatgtca aaagtaatgt taaagtttat gataaggaat
    11521 tgacagaaga gatttaccat gaaaataata ttattaagga tttaaaaaaa ggtctggaag
    11581 aacaaacact agaagtttgg tatcagccaa tttttaatgt tagtaaaaac tgttatataa
    11641 aagctgaggc actaattaga ttgagaaata aagagggcgg ttattattct gcctatgaag
    11701 ttattactta tgcagaaaag catggactta ttaaggatgt aggtctatat gtactaaaaa
    11761 aggcttgtga tgtaactaca aaaataaaag ataaaaatat agattttgaa caaatgcaat
    11821 taaatttatc tgttcagcag attgtacata agcagtgtgc aaatgacata ttatcatgta
    11881 ttgagaattg tggtctagat aaaaagtata tttgtgttga agttacagaa acagtactta
    11941 tggaaacttt tgaaattgta aatgttactc ttgataaact aaaaaaagaa ggcattagca
    12001 tagctttaga tgagtttggt tctggattta gtagtataaa ttatctggca aatttaccta
    12061 ttgaaaattt aaaaatagat aaggaattag tagaacaaat tacctattct aaaaaacaat
    12121 ttgaattatt aaaaactata gttcaaattg ctaagattaa tggcatggat attgtagcag
    12181 aaggtgttga aacaaaagaa actctggatt tagttgtaac ttcaggtgtg aatttgattc
    12241 aaggtcatta tttttcagaa cctttaagtg aagataaatt tattgaactt atgattgaag
    12301 aataaaggtt aataaaataa agatttaagg tattgaaaaa taattaaaag tatttaataa
    12361 tagaattagg gaagaataag tttataagta aaaataattg attagtagtt aataaaataa
    12421 atgtgatatc caaataaatc ctaaatctaa tttgagtttt aatatagata gtctaaagtt
    12481 tgattattaa agatttaatt gtaagttgtt acagttgatg atattgatta gagattaaag
    12541 tttagagtag aaagttacct taaatttaaa aatgcaagat aacccaatct aaaaattata
    12601 atcactcgat aggtttaagg atttatttgt ttattataaa taggagggtg tctcaaaatg
    12661 aactttttag tttatgaggc actctctttt atatagaatt aaatatattt tattatttca
    12721 acaatgaaaa agaagcttat ctctattttg agacagcctc tttgtatact attattaatt
    12781 taatcaataa cataatttaa aattatatta ttagttaaga tttcctgctt tattagtaaa
    12841 taattcttta atagcattgt ttctggaaac taaatcgtta tcagagttgt ttgttttttt
    12901 cataatcact gaataacgtt ttttatttat aaaaaatatt aaaatcttat catcatcaaa
    12961 attgtattct ttatatctgt caatagactc ataacctaga tttagaatgt ttttgtcttt
    13021 tatactatct acatctaaaa tatatgatgg gtcagccttt agtaatatat tgattaaaca
    13081 tttttcttca ccagcacaga ataactccat cttacttgat atagatttag attgctctga
    13141 agttattttt gcaggaataa ggttgtcatt atcatctctt agaagataac catgtgtgtc
    13201 ttgttgatat actaaattaa cctttttctt aggaacacta atcattttat ctaaatgtac
    13261 ataatgatga ttttcaacaa aaccttcatt taatatttca acaaagttat taattttacc
    13321 taggcaaaaa gattctatag attctttaca gaatttattt gtaaatggta ttatgtttac
    13381 gttttcctcg tctgtattcc atgctatagc ataatccaac gaatatactt cttcctctat
    13441 atctgcaaaa ttatattgta ctatgtttcc tttcaaaatt actcgacctc catcttataa
    13501 tatatttaat atactttata aaacttatat tatatttaaa taatatcata aattgaataa
    13561 atatgatata gtaaataaga aaaaatttga aaaaaatcac tataaatgag taataaatat
    13621 aaatagtgta tactaatggg gtggaaaatg tccactatta actttattac cccacatata
    13681 taagataaaa ttattaatat aaaatataat ctatcgacaa tgaatagact tttaaataaa
    13741 cttaatataa aattaattaa ataagtgctt aaaaacgatt tactttaatg atagaatcat
    13801 attaaaggag gataatttta tggatataaa gaataaaatt aaatctatat ttttaccaga
    13861 aaaaaatgac tatttagatg atgaagaatt tgagtatgat atagaaaata atgaagaaga
    13921 tttaaaggaa gataacaaag tacatttaaa tgatttaagc agagtaaaat atgattacag
    13981 agagttaaaa gacatagaaa accaaacaga agagatgtgt ctgctagcag taaagcagga
    14041 tggtactata attgagtttg taaaggacaa gacttataaa gtatgtatgg aagcagtaaa
    14101 acagacatat aaatctctta agtatataac aaatcaaaat gaggatattt gtatagaagc
    14161 agtaaaacag aactacagag ctttgtacta cataaataat aagacagaaa atgtactaat
    14221 tgaggctata aaaaatgcat caacttatga tgtcatggaa gtatttaaat ttgtggaaga
    14281 ggaaacagaa gatgtatgtc tagcatttat agaaagggca tctaaaaatg atgttgcaga
    14341 gatattaaaa ggaataaaag agcaaactcc agccatatgt ttagaagctg taaaaaaaga
    14401 tggaaaatct ttagcttatg taaaggaaca aagtaatagt atatgtttag aagctgtaaa
    14461 agaaaactac agtgcactat cttgtgtaaa agagcaaact gaagaaatat gtatagaagc
    14521 agtaaaacag aatgactttg cattgtatta tgtaaatgaa cagacagaaa aaatatgtat
    14581 ggaagcagta aaaagaagtt atatggctct acagtatgta aataagcaaa cagaagagat
    14641 ttgtttggaa gcagtaagaa ttgatggaag agcattacag tacgtaaaag agcaaacaga
    14701 agaaatttgc ttggagtcag ttagacaaaa tggaaaagta ctgcaatatg taaaaaaaca
    14761 gactgaaaat atatgtatag aagcagtaag aggttcattt gaagaacttg aaataaaaga
    14821 aatattaagt tatgtaaaga taccaacaga aagaatattt gtagaagcag taaagcaaaa
    14881 tggaaaaatt cttaaatatg tagaaaatca gacagaatta atatgtttag aggcagtaag
    14941 agaaaactat aatgctttag cttatgtaaa ggaacaaaca gaaaagattt gtttagaagc
    15001 agttaatcaa agttatgaag cgcttaaata tgtgaaagaa caaacagaag aagtttgtct
    15061 aaaagcagta aaacaagatt atagaatgtt gaaatatgta aataatcaaa cagaaaaaat
    15121 atgtttagaa gcagtaaagc aaaattatag agctttagaa tttgttgata atcaaacaga
    15181 aaaagtttgt cttgaagcag taaaacagaa tagaaaagca ctacagtatg taaaacaaaa
    15241 acaaagttaa aaggctaaaa tatatagaaa acatatgcaa ataaataaaa tttgatattt
    15301 tgtaataaat aaaatgggta tctcaaaagg aactttttag tttgtgaggt actcttttta
    15361 tatgaaatga aatatatttt tagcctttta acaatgggga aaaggtttat ttctattttg
    15421 acataggctt tttatgcata taaagcttga taaaattgtt ttattataat tgttttgtta
    15481 tataaataat aagaagaaaa ttatataggt ctttttttga ccagttttac atttttaaca
    15541 actagttttg taaagtatat tgaattttct caaaaaagaa tgtactctat atatgtaaaa
    15601 tttttttgga ggtaaaaatg tttaaaagat atgctgaaaa aatgacatct gttttaattt
    15661 gtaataatat gatagataat aatgaatcta aagtctattc ttatggattt gaaatattaa
    15721 tagctttcat agttaatata accaccatgc tgtttatagg ttttttattt ggtaaattta
    15781 catatgtatt atttttttta atgtgttatt gtctaataag acaattttca ggagggtatc
    15841 atgcagacaa ttattttaga tgcttattaa cctttatttt tataatttta tctacaatct
    15901 taattataga aaatataaat atagacttat ttaaaaatat tattatgatt attgcaagtg
    15961 ttagttgggt tggaatttgt gttctgtgcc caatagaaca taggagtaac cctataagtg
    16021 atagggaaaa attggtttat aagaaaactg ctatatttat atctacagta gtgttattga
    16081 taacaatttt aagtctttct ttgagtatat ttgtagatta tttcacatat agcgcatttg
    16141 ccatgttttg gatttttgtg atgttagtat taggtaagtt aaaagctaaa gtataagtaa
    16201 aatatgtatg agggagagtt aagttatgaa aaagtttatt gtaagattta tgaagtttgc
    16261 tagctcattg gcacttagca ctgctatttt gtcagctaat agtacctgtc cttggattat
    16321 tcatcagcct aaagtaccca aagaaatcag caatctaaag aagacaaatt aaatacaagc
    16381 acttatttat acaaaattat caggaaaaaa gaaattatta tattttaata agattaaaac
    16441 aatataaaat cagatacaaa atttatttta tttattttat aggagcctaa tacctatttg
    16501 gtaatatatt agtgcgccaa tttaggggga tatagtgtat ttaatgaaat gacttacgga
    16561 gagtaagtca tttttgtatt gtatgtattt tatgtaaact tatgatataa tgaagggtaa
    16621 agtttaatta aaataataaa aaactttagt atagatagta atttaggagg tagggtatga
    16681 gtatgataca taaattttct atgaatggat ataatatcgt tttagatgtg aatggtggag
    16741 ctgttcatgt gttagatgat gttgcatacg atttgttaga tttttataaa gaaaaaagta
    16801 aagaagaaat attagagata ttaaaatcaa aatatcaaga agaaaaaata aatgaagcat
    16861 atgaagaaat attaaatttg gaaaaagagg gtcttttata tacagaagat acatatcaat
    16921 atcatccaag ctttgtacat agagagcctg tagtaaaagc tttatgttta aatgtagctc
    16981 atgattgtaa cttaaaatgt aaatactgtt ttgcagctca aggtgatttt ggaggagaaa
    17041 aagaacttat gagttttgaa gttggtaagg cagcaattga ctatttgatt gctaattcag
    17101 gaagtagaaa gaacctagag atagatttct ttggtggaga gcctttgatg aactttgaag
    17161 ttgtaaaaca actggttgac tatggaagaa gtgtagaaaa agattataat aaaaatataa
    17221 gatttacaat aactacaaat ggtgtactgc taaatgatga gattatagat tatataaatg
    17281 aaaatatgca taatgtagtt ttaagtttag atggaagaaa agaagttaat gataatatga
    17341 gaccaacttt aaatgataaa ggaagttatg atattacatt accacgattt aagaaacttg
    17401 tggaaaaaag agcaaaagat aagtattatt atataagagg tacttttaca agagataatt
    17461 tagatttttc aaaagatgtt atgcattttg cagatttagg atttaaatta acctctgtag
    17521 agccagtcgt tggagatgag agtaatccat atgcacttag agaagaagat ttacctaaga
    17581 tatttgaaga gtacgaaaaa tttgctgtag agtatgcaga tagacaatta caaggagatg
    17641 gatttaaatt tttccatttt atgatagacc taaatcaagg tccatgtgta ataaagagaa
    17701 taactggatg tggagctgga aatgagtatc tttctgtaac tccaaatgga gatatctatc
    17761 catgtcatca atttgttgga aatgaagaat ttaaaatggc aaatatattt gatgaagaaa
    17821 tagtacttcc agaaaattta aaaaatatgt ttagagaagc tcatgtatat actaaagaag
    17881 agtgtaaaca atgttggaat aagttttact gttcaggtgg atgtcatgca aatgcgataa
    17941 actttaataa tgacatatca aaaccatatg aattaggctg tgaaatgcaa agaaaacgta
    18001 cagagtgctc tataatgata caagctaagt taatgttaga aggagcaacc aactaaattt
    18061 atattttaaa ggagttgttt atatatgatt agagattatt tagaagataa acctttaatt
    18121 gacgaaagtg tatttgtggc aaaatcagca gatgtaattg gaaatgttaa aataggcaaa
    18181 gattcaagta tatggtacaa tgcagtagtt aggggtgatg aaggaccaat aactattgga
    18241 gaaaacacta atatacaaga ttgttctata gtgcatggag atacagaaac tataattggt
    18301 aacaatgtaa cagttgggca taggtctata gttcatggtt gtaaaattag tgataatgtg
    18361 ttaattggta tgggttcaat aatattggat aatgctgaaa taggagaata tactctgata
    18421 ggagcaggta cactaattac atctaataag aagtttccac caggtgtttt gataatgggt
    18481 tctcctggta aggttgttag agagcttaca gaagaagata aaaaatatat tgatgaatct
    18541 tatgaatggt atttagaggc agcacaaaat caaaaatatt agtttaaaga gtggttgttt
    18601 caaaacatat ttaaatctgt tttgaggcaa ccactaattt attttattag attttgttga
    18661 tattttagtt ttcaaaaagt atttgattaa accagatata aaatgtcaaa aataaaaatt
    18721 aactgagtta aataatttta ataaaattgg tagtatatgt gataaaatta aatattaaag
    18781 aggatatagg atattagaat atcaattggt gaaataatgt catatacgat aaattaaggt
    18841 gtaagttttt gggtatacct atcttaggag tgaaaagatt tgagaataag ttatagaaat
    18901 ttaaagcgat atagagaaat tggatatgtt ttaataaagt atggttttag ttttattgta
    18961 gaaagactaa atatagaagg aattgcctat aaaataccac tatttgaccc accagaagaa
    19021 ataaaaaata tgacaactgg tgagagaatg aaaagagttt tagaagaact aggacctaca
    19081 tatataaaaa taggacagat attgagtaca agaaaagact tgttagacca ggatataatt
    19141 gatgaaatat caaaactcag agatgatgta gagaaatttg attcaaatat agctattgat
    19201 atatttaaag aagaagtggg tttaagtata gaagaaatat tcttagaatt taaagaagaa
    19261 ccaatagcag cagcttcaat tggtcaagtt tatgaaggtg tacttaaaac gggagaagaa
    19321 gtaatcgtaa aaattcaaag accaaatata gagaaaataa taaaatctga tttagagatt
    19381 cttagaacta ttgcaaatac tttaaaagat ttaaaaaaag attttaattt agatttagta
    19441 cagatgattg aagaatttca aactcaatta atgagggaac ttgattatac atttgaagct
    19501 ataaatgcaa ctaaattctc taggatattt aaaaatagtg atgaagtata tatacctaaa
    19561 gtatattcag aatataatac taagaaaatt ttagtaatgg agaaagttaa tggtacaaag
    19621 ttaagtgatg tagaaaaaat aaggagacta ggttataaca caaaaactat tgttgaaata
    19681 ggcgttagat cattttttac acaggtatta tctcatggat tttttcatgc agacccacat
    19741 ccaggtaata tattcgtagt tgctaaaaat aagatagcat atatagattt tggaatgatt
    19801 ggaattattg ataataaaac tttaaaccaa ttaaatgaaa ttgcattagc aggtgtagaa
    19861 aagaatgttg acaagataat atatctattg atagaaatgg atgcattaaa tggagaggct
    19921 gatattaagg gacttagaca ggatttactt tatctaatac attattacta tgatatttct
    19981 atagaaaaaa taaatgtaac agatatatta aatgaactat ttaggttttt tagacagtat
    20041 aaaatagtca tgcctgcaca atttgtaact cttgcaaaga cagttataac attagaagga
    20101 acaagtagaa ctttaaacac agatttttct tttggttcta tgggaaaaga gtttatgaaa
    20161 catcattata agagtaaatt taaccctaaa aatgttgttt taagttcaag acaaaatgta
    20221 gaagaaatat tactggacat aaaaactatt ccaaagcaat taaaagcaat tttaaaaaat
    20281 atagaaagaa ataatataaa aatgcaaatt gaagatgtta aaatgacgag gttagaaaat
    20341 tgtataatag agctaacatc tcaaatatct ttgagtttag tgttggcatc tattatagta
    20401 ggttcttctc ttataattgc atctccaaat atagaaaata atatatggat aaaatttact
    20461 gcaatagcgg gtttctttat atcctttata ataggccttt gcttagttat aagaagtatt
    20521 aggtcaaaat acaaaaaaga ttagggggaa ttaaatttga aaaaatggat attgaagtat
    20581 aaaggtaaga tagaggaaag tgaatttagc aaaaaactta atatttcccc ggaaatctgc
    20641 caaattttaa aaaatagagg gatatgtact gaaaaagatt cagaaatatt tatgaatcca
    20701 tctttagatt atttaagaga ccctttttta atgaaagata tgcaaaaagc tgttgataga
    20761 ataaagttag cagttgagaa gaacgaaaaa atttggattt acggagatta tgatgttgat
    20821 ggtgtatcat ctacatctat cttgtgctta tattttaaga gtataggtta caatgttgat
    20881 tactatattc ctaatagatt agaagaaggg tatggtataa atgaagatgc tattaagctt
    20941 ataagctcta ggggatgtga cttgattata agtgttgact gtggaattac atcagtgtct
    21001 gaggtcaata cagcgaatga tttgggcata gatgttataa ttacagacca tcatgaatgt
    21061 caaagtgaaa ttccatcagc ctttgcagta ataaatccaa aacaagaaga ttgtaattat
    21121 ccatttgatt cactatgtgg atgtggtgta gcatttaaaa tgattcaagc cttgactcca
    21181 aaagaagaat ttaaaactag tatgtataat tatttggaaa tagtaacact agcaactatt
    21241 tgtgatattg ttccacttat agatgaaaat agaataattg taaaaaatgg tttaaaatca
    21301 atgaaagaag gcaaaaatat tggattaagg gagcttataa aagtttgtgg tgtagaaagt
    21361 gataagatag gttcttctca tataggtttt gcaataggtc ctaggataaa tgcttcaggt
    21421 cgattgggat attcttactt aggagtagag ctatttacta ctcaatcaca agaagaggca
    21481 gtagaaatag ctagtatatt ggaagaaaaa aataatgaaa gacagatgat agaagctaag
    21541 atgtatcatg aagctgaaga aatgctaaag tctaatagca gatacaatga tgacaaggtg
    21601 ttggttttag ctaaagaggg ttggcaacat ggaataatag gaattgtagc atcaaagtta
    21661 actgaaaaat actacaaacc aacaattctt ctaggtatag aaaatgggga ggctactggt
    21721 tcagcgaggt caataaaagg atttaatata tttgaagctc tcataaagtg caaagacttg
    21781 atgactaagt ttggtggtca tgaacaagca gcagggttat ccttggattc agacaatgta
    21841 gaaattttag caaatgaaat aaataaattt gcagattata atttaactga agatgatatg
    21901 atagagaatg taaatgttga gtttgaatta caagaaaatg taataaattt aaatcttgta
    21961 gaagaattac ataagttaga accctttgga ttaaataatc ctaatcctag atttatagtt
    22021 agaaattata ttttaaaaga tttgaaagtt ataggtaaga atcaacaaca tctgaaatta
    22081 agtatagaaa aagagaaaag ctatgaatgt ataggattta atatgagtca tttaaaatca
    22141 atgtataaag taggagacaa ggtagatgta ctattccaat tagatgaaaa taactatatg
    22201 ggaaatagga aagtacaatt tttacttaaa gatatacgat tggctcgacc taaaagtgca
    22261 agtaatgaca aactttcatt gaaattaatg tcaaaaataa taccaaaaga tactcaaagc
    22321 ctgtataata tatctgtgtc tgattttgaa ttatttgatg gaaatacaga tataaatata
    22381 tttgattatt ttgaaaaaga tacactaata atatctaatt ctataaatgg attttataga
    22441 gcaatgtcag atatatcact tatagatttg gattttaaca taaattacaa tattattgaa
    22501 gatgttagca aaaatactga taaattagag cttatatttt ctcctaatat tgataaaata
    22561 gacttaaaaa gatataataa tattattctt tatgattatt tatacaataa aggcgaatat
    22621 tcgtatatat atgagaataa aagagaagaa agtgaaatta taaaatatta taataaaaca
    22681 gacctattat atttaaagaa tgtagttagt aatatagttc catcaagaga tgaatttata
    22741 acaatttata aacaagcact cataaaaaaa gaaattgatt tggatatggt aaatataagg
    22801 gaaactttta atgtaattcc attaaagttt tttacgatat taaatgtatt tagagaatta
    22861 aatttacttg actttaattt aaattatgaa aaaaattctg tactcataag aatattgcca
    22921 aaaccacaga aaaaattaga cttaaatgag agtttaatac tcaataatct aaaaaattta
    22981 gaaaaacaat acaatagcag ttattaggag gcgtttccca tggatttaaa aaactttata
    23041 aggaatattg atgatttccc aaagccaggc atagatttta aagatgtgac aactctattt
    23101 aaagatggag atgcatttaa atatgcagtg gattctatag ttgaagaatt gaaagataaa
    23161 gatgtagatt tagtaatagg accagaagca agaggatttt tgatgggtac acctgtagca
    23221 tatgctttag gagtaggatt tgttccaatt agaaaacctg gaaagttacc aggtgaagtt
    23281 gagagttatg agtatggatt agagtatggt acagatactt tagagataca taaggatgca
    23341 attaaaaagg gtcaaaaagt agcaatagta gatgatttat tggctactgg tggaactatg
    23401 gaagccgctg ccaagcttgt agaaaaatta ggtggagaag tagtttctat gcaatttttg
    23461 attgagctaa aattcttaaa tgggagagaa aagttgtcta actatgatgt taattctctt
    23521 ataaaatact agtactttta taatagttgg tataacacca actattttca tatatattaa
    23581 aattttatac aaaataggtg gttatatgca tgataaagaa ttacaagaaa taattgaaaa
    23641 aataaaagtt tatgcaccta atgcaaattt tgaaatggtt gaaaaagcct ataatcttgc
    23701 aaaaaaagcg catgaaggac aatatagaaa atctggggaa ccatacttta ttcatccatt
    23761 agcagttgca aatattttaa tagagatgga attagatatt gaaactataa cagcaggttt
    23821 acttcatgat gttgtagaag atacagaata tacctatgaa gacataaaaa aagaatttgg
    23881 caaagaagta gcggatttgg ttgatggagt aactaaactt ggtcaaataa aatatcgttc
    23941 gaaagaagaa actcaatctg aaaatttaag aaaaatgttt ttggcaatgg cgaaggatat
    24001 aagggttata cttataaaac ttgctgatag attgcacaac atgagaacat taaaatacat
    24061 gcctccagaa aaagcaaagt ataaagcaaa agagacactt gaaatttatg gtggtatagc
    24121 tcatagatta ggaatttcca agataaagtg ggaactagaa gacagagcat taagatttat
    24181 ggacccagaa gggtattatg atttagttag tagagtatca atgaagagaa gtcaaagaga
    24241 ggattatatt caaggaattg ttgaacttct taaagataag tttaaagatt taagtatacc
    24301 atgtgatgta tatggtagac caaagcattt ttacagtata tatagaaaga tgcaaaaaaa
    24361 acataagact tttgaagaga tttttgattt aacagcagta aggatattgg ttgataatgt
    24421 aaaggattgc tatgcagtac ttggtatagt tcatacatta tggagaccta ttccaggaag
    24481 atttaaagat tatatagcaa tgccaaaacc caatatgtat caatcattac acaccactgt
    24541 tgtaggtcct gatggagaac ctttagagat acaaataaga actcatgaga tgcataatat
    24601 agctgaaaat ggtatagcag cccattggaa atataaagaa ggtatctcta gtaaagagtc
    24661 aaaggttgaa gaaaaacttc aatggttgag acagatgatg gagtgggaaa aagatttaaa
    24721 agacccacaa gagtttatgg atgcacttaa agaagatgta tttaatagtc aagtttatgt
    24781 atttacgcca aaaggagacg taattgaatt accagcagaa tcaacaccta tagattttgc
    24841 ctatagggtt catacaaatg taggtaataa atgtgtagga gctaaaattg atggaagaat
    24901 agttcctatt gattataagc ttcaaaatgg taatattgtc gaggtactta catcatctaa
    24961 ttctaatgga ccaagtaggg attggcttaa tatagtgaaa accccaaatg caaaaagtag
    25021 aattagacaa tttttcaaga aggaaagaag agaagaaaat gttgaccgtg gaaatacaat
    25081 attagaaaga gaatttaaga agcatgatat accacaaaaa gaccctcttg ttgaaaaata
    25141 tatggcatta gttgctaaga aatttaatca gccaagtgtt gaggacttaa ttgccactgt
    25201 aggttatggt ggtataatgt catctcaagt ggtatctaag gtaaaagacc tatatttaaa
    25261 agaggttaaa aaagctaaaa aggaacaaca gatacaagaa gaagatttaa ataaacacaa
    25321 tataagtgaa acagaataca gtaaaaagag aaagaaaaac gcatcacaag gaattatagt
    25381 aaaagggctt gacaatatct tagtaagatt tgctaagtgt tgtaatcctc ttccaggaga
    25441 tgaaattgta ggatatatta ctaagggtag gggagtagcc atacacagag ctgattgccc
    25501 aaatgcaaac aataatggtg atttcttcca aaatagattg gtggaggttt catggagttc
    25561 agcaggaaaa ggtaagtttg aagcggaaat tcaaataaaa gctattgata gaagaggaat
    25621 tgttaatgaa ataactcata ttgttgcaat ggataaactt agtttaaacg gaataaatgc
    25681 aagaaaaggt aaggatagta ttgtaagtat aaacttactt gtagaggtta atgacacaga
    25741 ggaattaaaa attctaatga aaaaattaaa agcaattcct ggtgtagaaa gtatttatag
    25801 aatgacaaat tagagttaaa ataataggga ggaaactatg agagcagtag tgcaaagagt
    25861 atcttcatct aaagttacag tagatgaaaa cactataggt caaataaata aggggttact
    25921 agtattgtta ggagtgactc atgatgataa atcaagtgat gtagattata tgattgataa
    25981 aatattgaat ctgagaattt ttgaagatga aaatgacaag atgaatttat ctctaatgga
    26041 tataggtgga gagcttttag tagtatctca atttacttta tatggagatt gcagaaaagg
    26101 aagaagacct ggatttagta atgcagctag accagaactt gcaaataacc tttatgaaga
    26161 atttgtaaaa aaagcaaaag ataaaggtgt gacagttggt acaggtcaat ttgcagctca
    26221 tatgatggta gagttaacta atgatggtcc agtaacaata ctattagact caagtaaatc
    26281 tttttaaggg ggaaaacttt aatgattata aaaaaatttg tagatagtta ttttggagta
    26341 aatacttatg tattaggtga tgaaaagact aaaaaatgtg cagttataga cccaggtgga
    26401 agtttagtag agacattaag ttttgtaaaa gaaaatgaat taaatattga atatattatc
    26461 cttactcatg ggcatggaga ccatatagga tatgttaagg atattaaaga aaaaactggt
    26521 gctaaagtag ttgcacatgt tgatgaaaaa gaattattaa atgacaaaaa taaaaatcta
    26581 agttatacaa tgagatgtgg agcacaagag tttgatgcag atatctatgt aaatgataaa
    26641 gacaagcttg aacttggtga attaaaaatg actttcttgc atactccagg acatactcaa
    26701 ggatgtatgt gtataagagt tgaagatgaa atgttcacag gagatacttt gtttgctgga
    26761 agtattggta gaactgattt atatagtgga gactttaacc aaatggaaaa atcacttaag
    26821 aaattatgca aatatgaaga tgctgttaga gtatatcctg gacatggacc aaccagtact
    26881 ctaggggttg aaaagaagac aaatccttac atgtaatttt atattagtat ttaaaaaagt
    26941 ataaaatatt taataactat attagtgagg gggttcttct actgttaaat agaaggagtt
    27001 aagttgatgt tgtatgtttt tttaaaagga catgattata agtatgaagt agcagaactt
    27061 ataaaattgt ttaccagtga atttaagttt aaagactcta atgctaacaa taatatagag
    27121 tcaaagcatt taataaatag attgatttat gagaatggtg tattattctc aagtactgaa
    27181 tactatgaaa atggaaactt aaagtacgag tcaatacaga atatagaaaa tatgaatctt
    27241 gaatttttag atggatttga tagtagcaaa acgattgaag aatttcttaa tagcttagat
    27301 agtgaaagta agaggaactt taaaaagctg tgtaaagaaa atatcaaaaa aagcatgttt
    27361 gtagtgctta aacaagtatt taattcttat gtaccatggg gaattttgac tggtattaga
    27421 ccagtcaaaa ttgtccacaa tttaatggat aaaaagctag atgataatag cataagaacc
    27481 attctaaaag ataattattt tataatagat gagaaaatag atttagctct tgaaatagct
    27541 aaaagagagc gattatttat gtatcctata gataaaaata aaatttctct ttatgtaagt
    27601 attccatttt gtccaactag atgtgtatat tgttcatttc cttcaaattc attaaaacaa
    27661 tttggacatt taaaaagaga atatgtttat aaattgattg aagaaattaa ggggtttgcc
    27721 aaagttatta aggatactaa gaaagaagtt gaaactctat atattggtgg tggaactcca
    27781 actacacttg atgaggaaga attggatttg cttataaatt cattgttcaa tgaattagat
    27841 ttaagtaaaa ttaaagaatt tactgtagaa gcaggtagac ctgacactat tacagaacaa
    27901 aaactaagag tgttaaaaaa acacaatgtt agtagaatta gtataaatcc tcaaactatg
    27961 aatgatgata ctttagtaaa gattggaaga gagcatagag tatctgattt agtagattgc
    28021 tttaatatgg ctcgtagaat gggatttgat aatataaata tggatataat tttaggtctt
    28081 atagatgaag acttaaatat ggtaaagaac acattggagg agataaaaaa actttctcct
    28141 gaaagcctta cagttcatac attagccata aagagagcat ccaacttaaa tataaatatg
    28201 gataggtata aagaacatct aacacaatat gaagaaatgg taaaaatgat agatttatca
    28261 atgaaatatg caaaagatat gggattaaat ccatattata tgtacagaca aaaacatatg
    28321 ttgggaaatt tagaaaatat aggttatgct aaagaaggat atgagtgcat ttataatata
    28381 cagattatgg aagaaaaaca aagtaactat gcattaggtg caggtgctat atcgaaattt
    28441 gtttatgtag atgaagatag aattgaaaga gtagaaaatg taaaaaatgt tgaacaatac
    28501 attgaaagag tagatgaaat gataaaaagg aagaaagaag aggtatataa aaatgttgac
    28561 taaagctcct agaggaacta aagacataac tccaaaagag gcttacaaat ggcgttatgt
    28621 agaaaataaa tttagagaaa tatgtgcttt atatggatat gaagaaatgg taacacctat
    28681 atttgaacat acagaacttt tcaagagaag tgttggagat actacagata tagttcaaaa
    28741 agaaatgtat tcttttaaag ataaaggtga tagagaaatt accctaaagc cagaaggtac
    28801 agcaggtgta gtaagagcat ttatagaaaa taaattatat gcagatactc aaccaacaaa
    28861 attattttat gtgacacctt gttttagata tgaaagacca caagctggta gacaaagaca
    28921 atttcaccaa tttggaatag aagcacttgg aagtgatact ccttcaatgg atgctgaaat
    28981 aatcgcacta gctgttcaat tttttaatga agttggatta aatgatttgg tagtgagtat
    29041 aaattctgtt ggttgtccag tttgtagaaa agagtacaat gctttattaa aagagtatct
    29101 tgactcaaaa gctgatatat tgtgtgatac ttgcaatgag agaagagaaa aaaatccaat
    29161 gagggttata gactgtaaaa atcctacatg taaagaaaat atcaaagata taccatttat
    29221 tgcagaccat ttgtgtgatg attgtaaatc tcactttgat aaacttcaag aatacttaaa
    29281 ggaaatgaat ataaactttg taattgataa aacaatagtt agaggcttag attactatag
    29341 aaaaactgct tttgaaatta tatctaatga tataggagct caaagtactg tatgtggtgg
    29401 aggaagatat gatggtcttg ttgaacaatt aggaggacca aaaggtataa gtggaattgg
    29461 gtttggacta ggaattgaaa gacttttact tacacttgaa ggtaatggaa ttgaaataga
    29521 aaaccctcaa tctacggata tatttatagt aactataggt gaaaaagcaa atacaagaag
    29581 ctttaaattg ttaaaagact tgagacaaaa tcatataagt gcagataaag accatataga
    29641 gagaagtgtt aaagcacaat ttaaatattc agataaaata aattcaaaat ttacaatagt
    29701 aattggagat gatgaactta aaaatgacac tgcgacatta aaaaatatga aaacatcaga
    29761 gcaaactact gtaaaattaa gtacattagt tgaagaatta aagcaaaagc tgtaaaataa
    29821 gaaagtatga tgttttgtgt attagataaa tatatagcat taaaattaaa taaaatggag
    29881 gtagtatctt ggaaacttta aagggtttaa agagaactca ctactgtgga gagttgagag
    29941 aaaaaaacat aaatgaagaa gtcgtactta tgggatgggt acagaaaaaa agaaatttag
    30001 gtggtcttgt atttgttgat ttaagagata caagtggatt atgtcaaata gtttttgata
    30061 cagatgtaga taaagaagct tttgaaaaag ctgaaaaatt aggggctgaa tttgtaatag
    30121 ctgtaaaagg aaaagtatgc gaaagacaat ctaaaaaccc taatatgcca actggtgata
    30181 tagaaatatt tgcaactgaa cttagacttt taaacaagtc agaaactccg cctatttata
    30241 taaaagatga tgatgatgtt tctgaagcac taagattaaa atatagatac ttagatttaa
    30301 gaaaaccatc tatgcaaaga aatttaaaat taagacataa agtaatgaat attacaagaa
    30361 attacttatc aaacaataga ttttgcgaaa tagaaacacc atttttaata gctccaacac
    30421 cagaaggagc aagagattat ctagtaccaa gtagagttaa tccaggaaaa ttttacgcat
    30481 taccacaatc accacaatta tataaacaat tattgatggt aagtggtatg gatagatatt
    30541 tccaaatagt taaatgcttt agagatgaag acttaagagc agatagacaa ccagagttta
    30601 cacaaataga ctgcgaaatg tcttttgttg agcaagaaga tgttatgagt atgattgaag
    30661 gtttgcttga agcgatattt aaagaagttt tagatgtaga attggcactt ccacttccta
    30721 agatgactta tgcagaagct atgtctaaat atggttcaga taaaccagat actagatttg
    30781 gatatgagtt aacagatata tctgatgttg tttgtaactg tggatttaaa gtgtttgctg
    30841 atgctacaca accaggaaaa agtgttagag gtataaacgt aaaaggcaaa gctgacgact
    30901 ttactagaaa acaaatatca tcactagaag aacatgctaa gacatataga gcaaaaggtc
    30961 ttgcttggat gaaagtagga caagaaggag taacttctcc aatagcaaaa ttctttaatg
    31021 aagaagaaat gaacgctata cttacaagaa tgaatgctga agtaggagat ttacttttat
    31081 ttgtagctga taaaaactca atagtatttg atgctttagg acaagttaga cttgaagtag
    31141 caaataggct taatctattg gataaaaatg tatataactt actatgggta actgagttcc
    31201 cagtttttga agaagatgaa gaaacaggca cattctcagc aatgcatcac ccatttacat
    31261 cacctatgga tgaagattta gacaagttag aagaaggtga taagtcatct ttaagagcga
    31321 aagcttatga tattgtattg aatggttatg aaataggtgg aggaagtgtt agaatatcaa
    31381 attctgatgt tcaatcaaga atgtttaaag cacttggttt tacagaggaa agagctaatg
    31441 aaaagtttgg ttatttatta gaagcattta aatatggtac tcctcctcat gctggtctag
    31501 cttttggtct tgatagactt gttatgttac ttgcaggaac tgataatata agagaagtta
    31561 ttgcattccc taaaaatcaa aatgctgttt gccctatgac aaatgctcct acattggcgg
    31621 aagatgagca attagaagaa ttaagtataa aagttgatat aaaagataat gaataatatg
    31681 tagttgcaag aaattaaaat gtaaaaagtc tggatataaa gtatatttat attcagactt
    31741 tttttattag aattatttta tattttttct ctatatattt tcataaatta tacttgtatt
    31801 ctcaaccaat ataattaaaa tactatgttt aaaaatttta cataaacttg gttgttttag
    31861 aaactacttc tgtaatgagt acacgatttt aattaaagaa tatataacaa atttgaaata
    31921 agtctaatat atgacttaat agattaaatt aaaataataa atttggacta tttatctatt
    31981 tataacttaa attatatcaa attgataaca aactagatat tatacttttt tgtagaatag
    32041 aattacaatt atataaagaa aaattctatt aatagtaaaa aaaaagtaga aaaaagtaga
    32101 aattattctt ttataaagaa agggagggat aatattgcaa gagagagaag aattagtttt
    32161 aaatccagtt ccaaaagagg aaagagttgg atggttagct ccattgttta atatgcttgg
    32221 ttctaacata gctatatctg aactaatggt tgggggaact ttaattttag gaatgactct
    32281 ttctaatatg attattacat caattatagg gaacttaata ttagttgcaa ttattatgat
    32341 acaaggttat ataggttaca aagaagggtt aaatacgtat gtattagcta agggggcatt
    32401 tggagagata ggaggaaagt atcttatatc tctattactt ggtataacta gttttggatg
    32461 gtttggtgta caagcagggg ttgcaggact atcagtacaa aagatattcc caagtgtaaa
    32521 cctaacatta gttacagtta tacttggatt atttatggtt gtttttgcac tgtatggttt
    32581 taaagctatg gctaaattta attacttagt tataccacca cttatgatat taatggtgtg
    32641 gggagttttt aaagcttttt ctacttatgg agttgaagct atttataatt atactcctca
    32701 aacaactatg agtatggttg aaggtcttaa tattgtagta ggtcttgtta tagttggtgc
    32761 aattatatca cctgaccagt taagatatac gagacgagta aaagatattt ggattatcag
    32821 ttttttaggt ctagggatta tttcattatt ccaacaagtt gcagctggtg taatgtctat
    32881 gggagcacct acttgggata taacagaagt attggctaat ttaggttttg gatgggtagc
    32941 gtttgttatt ttaatacttg catcttggtc tacaaacgta tcaaatgctt attctggtgg
    33001 attagcatta aaaactattt ttccaaatgt aaaaagaaat gttttgactt tagttgcagg
    33061 tctaataggt acaataatag cagctactgg tataatattt aaatttcagt catttttatc
    33121 tttcttggga atcgcagttc ctgcaatagc tggaattatg tggtgtgagt attattttat
    33181 acaaggaaga acttataagc atagagaagg aataaattgg attgcagtaa taagctggtt
    33241 aataggtttt gcagcgtcgt attatagttc aaaaataaat ttcttaatac ctccgataaa
    33301 tggaattgta gtgagtatgg ttatttatta tatattaatg aaatgttttg gaatcaaaga
    33361 taaataaagg gggcaattac atgggtcgta aggtgaaaat aggtataatt caacaacaca
    33421 gtgtacttgg agatgttaaa aaaaatatag aaaaggcagt agagatgata gatgacttag
    33481 gaaaacaagg ggcagatata atttgtttac cagaactatt tgctacagga tataatttag
    33541 agtctttagg tggagttaaa acattagaat taataagaga acataataag tacatagagg
    33601 aatcaatgtc agaagctgct aaaagaaata atgtatacat gatatctcca tatggaacct
    33661 tagaaaaagg gtctacacat gtttataatt cagcagttat atttgataga aaaggtaaga
    33721 ttatgggaga atactgtaaa aatcatctgt ggtcattaga agcagtatac tttaaaggag
    33781 gggaaaaagt agaggtatat gatgctgatt ttggtagatt tggtgtaatg atatgttatg
    33841 atgcaggttt tccagaagta tccagagaat tgacattaaa aggttctgaa ataatattta
    33901 taccatctgc atggagaata caagatgaag atatgtggga tttaaatgta tctcaaagag
    33961 ctttagagaa cactgtatat acagtaggtg taaatttagt aagtaatgac tctaatttaa
    34021 tactatttgg aaagagtaaa atatgcaatc ctagaggtac tgtaataact caattagata
    34081 catacaggga aaaatatgta ttatcagaga ttgatttaga tgaaataaaa aagtataggg
    34141 aagatatccc ctatttgaaa gacagaaaaa gtagctataa catataattc tatactataa
    34201 tacaagttca atataaaatg ataaaaagag gatgtatcaa tattaagatt aaatttatta
    34261 tactttgata tatcctcttt aatattatca actttgattt aaatattttt aaaatagttg
    34321 ataagaaagt ttttgaacaa caacacagct ttagattcat agttttcgct aaaccatgaa
    34381 agatttaaat atcttttaaa ttcaatatct ttgatatgta acaacttaat ctcagggtct
    34441 tgagataatc cccatgattt tccacatata aatccaactc cttgaagtga ttttataaga
    34501 gcataaattg tatatggtga atcagactca aatgctattt tgggtttgaa attagctgat
    34561 tcgcacagga tatcaattac ctctctataa ttctcaccct tcgttataac tataaagttt
    34621 tcatctgaaa cttcgcttag atatacttca tcctttaatg ataaaggatg gtttattgaa
    34681 actcctaata gtatttcttc gcatgttaaa gttatgctat tttctgagtt tagctttgta
    34741 aatgaagaag atatgtacaa atcaaaatca ctttttttat aagatgatgg caaagtatgt
    34801 gacacattaa aagtaatatt gggatatagt tttctgaaat tactcaaaag tgatggtaac
    34861 acattagccg ctgcaggaga taatatcttt acttctcctg aattttctaa agaagtgtct
    34921 tgtaactcct tttttgaact ttctactaaa tctaaaatgc tatctactct ttttagaaac
    34981 aatcttccat actcatttaa aacaatatat cttccttttc tgtcaaataa ctgaactcca
    35041 agttcttttt ctaaggaaga tatagttttg cttaaagctg gctgtgctat atttaacttg
    35101 tgtgatgctt ttgttatgtg ctccattcta gcaacagttt gaaaatactt aagatgcaat
    35161 aaatccatag tataaaatac acctctttta atatgacttg agttatatca attttataat
    35221 caactccaca ttatacatta taatataaag tttatataat aaactacaat atattaaaaa
    35281 gttatttaaa ttgttattta tattttgtac ataatctaat aaaaaaaagt aactttaaat
    35341 gattaactaa ggaaatgttg ttacaaactt tagttatata aaaaattcat ttgatgagca
    35401 aagtactaat atcaagtaag aataaaagct tattagataa gaaaaataat attttataat
    35461 aatgatgtta cttaaattat tatgttatac tattttaaat aggaaaaatt tagatactat
    35521 aattttaggg ggagtcaaga tgaaattaac taaatcaata tcatcaatgc tgtgcttatc
    35581 tctagtttta agcttatcac cagtagcata tgcaaatgaa ataagtagaa caacagttga
    35641 gaatacttta gtaggtcagg atagatatca aactgctgtt gaagtaagta aaaaaggatg
    35701 gagtagtgca aatgaggctg tagttgtaaa tagtgaatct attgtagatg cattagctgt
    35761 aacaccattt gctaaattaa aaaatgctcc tatattattg acagaaaaaa ataatctaaa
    35821 ttcacaaact gagaaagaat taaaaagatt aggagttaaa aaagtttata taattggtct
    35881 atctagttct gtatcaaatg atgtgcaaaa acagttagaa tctaataatt taactactga
    35941 tagagttggt ggtagtgata gataccaaac agctttgagt atagctaaaa aaatagaagg
    36001 attaaaagat atatcagaaa tagctgtagt aaatgggtac acaggtcttg cagatgcagt
    36061 tagtatagct tctgtagctg ctactaatgg aatggttata cttccagtat ctgatgcatc
    36121 tggtatatct agctttaaag attttataga atctaagaat ataaataaat catacataat
    36181 aggctctact aatgctgtat cagataaaat aaaacaaagt ttacctaatt cagaaagaat
    36241 aggtggagca gatagaaatg aaactaatgg taaagtaata gaaaagtttt atacatctaa
    36301 taaattaaac aatgtatttg tggctaagga tggaatgaaa aatcaaaatc aacttataga
    36361 cgctctagca gtaggagtac tagcatcaaa agaaaattct cctgtactaa tcgtaggaga
    36421 acaattaagt gcaacacaaa agagtgtatt ttcgaataaa aatgctacct ctataactca
    36481 agttggtagt ggaggaaatg aaaatgcatt taaagaatta aaagatttac aggcaaatag
    36541 taacaataag agagtaatgt atgtaactaa tacagataag gtaaatataa gaaatgatgc
    36601 tacaatagaa gcttctgtta taggatatct aaataatggt gatgaagtag aagttttaga
    36661 tgtgttaaaa actggatggg taaaaataaa atacaatgaa ggtattggat atgttagtgg
    36721 gtcttatttg acaaataata agcctgataa tagtaatgaa aatataaaaa taaagtatgt
    36781 aaaagaaaaa gatggtctaa atgttaggaa aggaccaagt acagaagatg aaaaaatagg
    36841 tcaactttca tatggaagca aagtagaaac agtagaaatg tttgctactg gatgggtaaa
    36901 aataaagtat aatggtggat atggttatgt aagtaatgat tacttatctg atacaccagt
    36961 tatttaatta taagtaacaa taaaaaaact gctttaattt gtaaagcagt ttttttataa
    37021 aaatttaagt ctaataacta tttctatttt gataccttaa tcctgggatt tattattatg
    37081 gatattatca acatacaaac actccaaatc cagtgtctaa ttccaaactt agatattaat
    37141 aaattcagtc ctgctatggt aaataagcag tatttaatta caattaaaag tgtatttata
    37201 gaaagcccct tcatagaaag tatcctaaat ccaataatag acattctaag tcctataaat
    37261 gccaataata tacctgcaat atcctgaatt aagtaatagt attcaaattt catattcaat
    37321 caactcttct ctgagcagta tcaaatattt ctaactcttc aatttcatca tctgtgtatg
    37381 taagcatagt aaaaccagtt acaccataaa taaatgaaat tataggtgta atccaactta
    37441 agaaacaata tggaataaat tctagtgggc ttacaccaag agtctgagca cagaaaacag
    37501 catttgaatt ccatggtatt aatggaccac caagtgtagc tgtatcttct ataattctag
    37561 atagattttg tggttttaat ttaaattctt tgtatattgg agacattaga gttccgacaa
    37621 atacagaagt gaacatcatt gatgaactaa atgcatttgc aaagtagctt actataaaag
    37681 tagacccaac caatttagtt cttgattttc ctattttagt tataagaggt tgaagcagta
    37741 cttctaagaa tccagttttt tggagaatac cagcaataac gaaaactaca aatactagta
    37801 aaacggtttc agccatactc attattccac cacgatttaa tagtttgtcg gcatagacaa
    37861 atccagtatc tatagtaaat cctgatagca tgtaatttaa aacatcacct attttttctc
    37921 cttcttgaaa tactgctata ataagtccca tgatagctga acttagtata gatataatgg
    37981 gtggcttttg tagcaataaa agtaataata aaaatattat tggaatcatt gctactaaac
    38041 caatatgaaa gtttgaattt aatacatcct tgatttgatt tatttgaaca tagtctatag
    38101 tattatttga gtacttaaat ccaattactg tatacagaac tatacatatt acgtatgcag
    38161 gtcctgtggt ataaaacata tgcttcatat gtgtaattac atctgtctta caaactgctg
    38221 cagctaagtt tgtagaatct gatagaggtg acatcttatc tccaaagaaa gcaccagaaa
    38281 tgactgcacc agctgttaaa ccaagaggaa tacccatact agtaccaata ctcatcattg
    38341 ctataccaga agttccaaga gtcccccaag atgtaccagt agcaacagaa gttaatgaac
    38401 atacgattaa tgttgtaagc agaaaatatt taggggttat tattgttagt ccactatata
    38461 ctactgttgg tattgtacca gcagctatcc aagctgctat tagtacacca acagaaagta
    38521 taattaagtt tgattgaaag gcatctttac caacttcaaa tccaaaagct tctatttcat
    38581 cattagtata accaagtttc atggctgcag gaactactat aagccatgaa agtaaaaaca
    38641 ttgtagttat agaagcatta aatacaacta taccaactga tgttagggct attattgcaa
    38701 gcattactaa caaggaatat ctaaatgaag gtatttttct ttctgtattc attttcatag
    38761 tgcaaattct ccttaaatat ttttagttaa ttgtattttt tattcttata agagcttctt
    38821 caagcatact tcttggacaa gctaagttta ttctttgata cccacttcct ccaatgccaa
    38881 agctgtttcc ttgatttaga gcaactttac ctttttgtac aagtatactt tctagttcct
    38941 catcacttaa tccaagtgca ctaaagtcta cccataataa ataagtacct tctggttttc
    39001 taactttaag ttttggcata ttttcattta tatattttat agcaaaatct atattagatt
    39061 ctaaatattc taaaaatgat tctagccaag attcaccatt attatatgaa gcttcagttg
    39121 caactaagct aaagcaatta tttctcttta tatcaattct ggtaaaggca tcatctaaca
    39181 atttatagtc tttttcatca ggtagtatta catatgatga ttgaagacca gctatattaa
    39241 aagttttagt tggagccata caagtaattg tatttttttc aaattcttta gaaatagatg
    39301 ccataggaat atgcttatgt ttttttaata taatgtcact atgtatttca tcagatatga
    39361 tttttacatt atgcttaaga caaatatctc ctagtttttt taattcatct tttgtccata
    39421 ctctaccaac tggattatga ggattacaaa gtatgaataa ttttacatct ttaatcttgt
    39481 tttcaatgtc ttcataatcc attatgtagt ttccattttc tagtttttgt aatgggttta
    39541 taattagttc tctattgtta ttttttacaa cactattaaa agggctgtaa actggttctt
    39601 gtatcattat tttatcattt gcttttgtga gttcatttat taaaagactt atagctggga
    39661 taacacctgg actataaatt aaccactcac tttttatttt ccagttatgt ctcctatata
    39721 gccagtttac tatagattca ttgtaagaat ctggtcttgt tgtgtagcca taaatctctt
    39781 gttctagtct attttttaag gaatcaatta tacaaggtgc agccttaaaa tccatgtcag
    39841 ccacccacat aggaagtaaa tcattagttc catacttttt ttccatttct gaccatttag
    39901 atgaaaaatt attgcttcta tcaacaatct cattaaaatt ataattcatt gtaaatacct
    39961 ccagtaattt taattttatg ttaataatat taagcaaaca acgtgccaat attgataata
    40021 aaaaatattt attcacaagt taatatttaa tattaagtaa tttcagagca tagataagat
    40081 aaaaatatta attatactta aaactttgtg gaaaaggtta tttctaataa atttttaaat
    40141 atatgaaaat gtaattaaat aggttaaata ggtttaactg tttaacttgt tagacctatt
    40201 tatatttttt agaaattcta ctccttttga tgttataatt gaacctccac gaccaacaag
    40261 tatgtctaca agcccaaagt cttgtagttc taaaagcata tttctaatct gttgttctgt
    40321 caaaaatcta ttttcctcta aagctatttt acataagctt tttctaccta ttctttcttt
    40381 taaatcataa gcctttttta aattatccaa tataaatatg tattcatcta gtgacctttt
    40441 aaaattatag tcgtatttca tgatgttttt gtctttgcca atgttgaatt gatacttttt
    40501 tatattatca cttactttat tacaaacggt tctactataa tttgaatcta tagtatccaa
    40561 tatatattca ggtaaatcac atatttctat aatatctttt tctaagtaac agaagtattc
    40621 tcctaagttt cttaattctc ttacatttcc ttcccatcta tacattttaa agatttcttt
    40681 tacttcctca cttagaataa aattacatgg aatatcatat tttaatgaac caaaaatttc
    40741 aaatatatca gcaggtcttt ccctaagtgg atgtatttta aggggaagaa cattcaacct
    40801 aaaatataaa tctcttctaa acatattttt agatacaagt tcttttaaat ttctattagt
    40861 agctgcaatt attcttacat caatgtcaat gacattatta gaaccaatac gtcgtacttg
    40921 tttttcttgt atgactctaa gtaatttaga ttgagaattt aaatccattt ctccaatttc
    40981 gtctaaaaat atagttccat tgttggctaa ttcaaataaa ccaattttcc cacctttttt
    41041 agcaccagta aaagcccctt catcatagcc aaataattca ctttgcagta gattttcttg
    41101 aaaagtacta caatttactg cgacaaaagg accatctttt ctcctagaag cactatgaat
    41161 ggattgagca aataattctt taccagtacc actttcacca attataagta tagatgaatt
    41221 tgattgagcc attttattag caattttttt tgtattaata gtttgtatac tagaacctaa
    41281 tatatcatca aatgtgtatt tactgatatt accagagttt actagttgtg ctcttaattt
    41341 agcttgcttt ttttctaatt gagaaaactt tgtcatcttt agtataaatc catcaaatac
    41401 atttaattgt atatgtttaa tctctaaatt tatatcaata tgatttattt taatcaattt
    41461 ctcaaaaaat ggagttttat tggtcaatat gctttgaaaa ttgatgtctt ttatgcaatc
    41521 accaacaaat gagtcaatca tttcatttgc atttatagac aaaatttttc tagccatatg
    41581 attatagaat tgaattattc catcgttatt tgtgcatata attccatcat caattataga
    41641 tagtaacaaa tcaaattgtc tttcaaactt atttgcatct aacaataact cacctgtact
    41701 ataacttgta ggaattattt tttctacgta tttttttatt aggtcatctt ttattagatg
    41761 ttttaatttg gtttttgtag caatttcaac aatacaactt aaatctatta ttctgtattt
    41821 taaatctata acttcacaat ttttaaactt aggcaagatt ttttcgccag gagttaggat
    41881 tacagcatct gtgggagtaa actcaatctc tggatagcag ggcaggatat ttatattgtc
    41941 tattcctaat ctatgtatta aagaaattgt ttctattgcc atatctttac ttaagttata
    42001 tacataaata attttatctt tttctaattt ggatatcata tcaatactgt tcttttcaaa
    42061 tgtcaacttg ggaacaatga tttgagcatc atagtgagat aattttacta tttcatcata
    42121 ttttaaataa gcagatagga gtataagttt ttctttaaaa aactttaaat ttccctcttc
    42181 aaatgagtat aaatttatct ttagattatc acctaagaat tgccttattt gattttcata
    42241 aatttctcca gcgtcttttt ttaaacttac aatagctaat tccatttgtt tcattttaac
    42301 ctctcataca aatttgactt attttagatt acataataat taaattaaat aatattattt
    42361 aattattatt ataaatttat tatagactac acattgaagt aaaagaagta gctaaaatta
    42421 ttccttttat ttttgttttt tgtatctatt tggtataata gtattagagc gtataataat
    42481 ttaggcaaaa ttttttaagg aagactagag gagaatataa aatgaaaaaa tgtctttact
    42541 gtaataggga tataaagtca ccaaataata aattagctat aaaatatgat gacaatatga
    42601 atatatatcc atgtcgtgca gaatgtaagg aaaaaattga agaatatata gcaaaaaaac
    42661 ctacatataa gtttataaat atattagagg ttcttttagg tgtgggcgga atattctgct
    42721 ttttgagtaa atggactatt gcagcccaag ttgtacttgg aattgatgca ctggttatat
    42781 ttttattccc attggcagga tttaagatga atggaaagtt aagtatggaa caaactaaga
    42841 atcaaagtag gtctattgca atatcaattt tggcatttat agtaataata actgtattat
    42901 attttaaaca agttggtaag tagagcataa ttgtataaga catcattaga gtatgaggtg
    42961 agtttatgga agaaagaaaa ttagcaccaa acaatgagag ggaagctctc ataaaaagat
    43021 taaaaagaat agaaggtcaa gttaagggta tacaaaagat ggtggaacaa gaacgttatt
    43081 gtgttgatat attggttcaa atatctgcta taaggtctgc tataaataga gtaggcacaa
    43141 ttatcttaga gaatcatata aaaggatgtg tagcagaaag cattaaacat gatgatgctg
    43201 ataaaactga agagatgata aaagagctta tggatactat aaataaattt acaaaataag
    43261 gaggcggtta gttatgaatc aacaaggatt tattatggaa attgttgatg atagaactgc
    43321 aaagttaaaa atgcaaagac attcagcttg tgctgcttgt ggaaaatgtg ctactacaaa
    43381 ttcatcagag tcaaaagaaa tattagtaga agtagacaac acaattggtg caaaagttgg
    43441 tgaccatgta gaagtgagta tggataatat gaatgtatta aaagcagctg taatggctta
    43501 tataatacct cttatagcac ttcttgtagg aacaataggg acttattata tattgggggc
    43561 aattggaatg acaactggag ttgaagccat aagtggatta gttggaattt gtcttacaat
    43621 aatctgttat ttatacttaa agaagaatga caaaaaattt agagacagta gagaatttat
    43681 accagtaata acaagaatat taattgacct ttaagataat tataattaaa aaatagcaat
    43741 gtgcttttta aataaatact caatacgtat ttgtagagtt taagtacatt gctattttta
    43801 tatttttatt ttgattttct gtaaggaata aagttttttc tgatttttaa aagattcaat
    43861 gttcttacaa aagaagttac aactaataca ccaagtgcta ttgcagcact tagaaatagc
    43921 gcatctgtac cttttgacat agccaaagaa taatttttct gaatcaaaga caacattgta
    43981 ttatatagcc caattcctgg tattagagga agaatacctg gaattacgaa cacaatagct
    44041 ggttgcttta gttttcttgc taaagtctca ctgcacgctg agaccaaagc agcagcgata
    44101 aaacctgcaa aaactgcatt tgtagtaaaa ttaaataaat aaacatatac aatccaacca
    44161 attccgccag ttactccagc tggaagaagt agatatagtg gagcattaaa gaaaacagca
    44221 aatccagcag ttgcaacacc tgaaaataaa aaatgcaaaa tcatagacat atcagtcatt
    44281 agaaaggacc tcctattcta taccatgtat caaggacaaa accaactcca gaagcaattg
    44341 caataataat catagcagca tcaaaagcac gagaaattcc tgatattaaa ttccctgata
    44401 ttaaatctct aatagctttt ataaaagcaa ctcctgggag tagtggcatt atagcgccga
    44461 ctataagcat tttagggtca tctaaaatgc ctatttcaac tagtagaact cctgaaagtg
    44521 caattaaaaa agaagatact aaacaggaaa atgcagatat tgcacttatc ttcatcgttt
    44581 tatcataagt aattgcagca aagattgcta ttataaacgt aagtataaaa tttaatacat
    44641 aattccctcc aagcaaacaa gcaaaacaag cagatgcaat tcctgtacaa gagtaaaata
    44701 accataaagg ataaggtttt acatcttgga tttcataaag acgtgcaatg gcattatcaa
    44761 tatcatactc tttgtcacta acaaattctc gagaaaaact gtttaataaa gaaatcttat
    44821 ttaagtttat acctcttgat tgaatcgttt tcataaacga aaagccatca aatttctcat
    44881 ctgaaataat tattactgta ggagttgtaa aaatatttac atgataaaac cctcttgacc
    44941 tgcatattct aaggacactg tcttcaaccc tagatgtttc agctccattt tttatcatca
    45001 gttcccctac aaatagagct agttttagaa tatttttctt atattcaagt tcctcatttt
    45061 gattcataaa tacacctcca aattaaaaaa cgtaatattg aaattatatc aaatttttaa
    45121 gtaatattcc ttaatatcta tacaaatata ttatcttttt tttgtttcaa aatagtcaca
    45181 tttaaattat atccaattaa aagaattaga caatattatt tattatattt taacttaaaa
    45241 ataaagaaag taactatgtt aaatatggaa aaatggcaaa acacttgact attaattggt
    45301 aaaggactat aataataaat tatcatattg ataaaaacga aaattatgaa atgtctgtaa
    45361 aaataaaatt taaataataa ctaggaggag taatatgttt gagaacttga aaaaagcgga
    45421 ccctgagatt tatgaaagta tgaaaagaga gttaaaaaga caacaaagaa atattgaatt
    45481 aatagcatca gaaaacatag tatcagttcc agtaatggaa actatgggaa gtcatcttac
    45541 aaataagtat gcagaaggtt actcaggcaa aagatattat ggtggatgtg aatacataga
    45601 tgaagttgaa actcttgcaa tagatagaat caaaaaaata tttggagcag aacatgctaa
    45661 tgttcaacct cactctggag caaatgcaaa tattggtgta tattttgcta tgttaaaacc
    45721 aggtgatgtt gttatgggaa tgaatttatc tcaaggtgga catttaactc atggtgctcc
    45781 agttaatatt tctggtcaat attataaatt ttatgaatat ggtatagata aagacagtgg
    45841 acttatagat tttgatgaag ttagaaagat agcacatgaa gtaaaaccta aaatgatagt
    45901 tgcaggagca agtgcatatc ctagagaaat agattttaaa aagtttagag agatagctga
    45961 tgaggttgga gcattgctta tggtagatat ggctcatata gcaggtcttg tggctgctgg
    46021 tcttcaccaa aatccatgtg aagtggctga ttttgttaca acaacaactc acaaaacttt
    46081 aagaggacct cgtgggggtg ttattctttg taaagaaaaa tatgctaaag acatagacaa
    46141 agcaatattc cctggtattc aaggtggacc tcttgaacat ataattgctt ctaaggcagt
    46201 ttgctttaaa gaagctttaa gtgatgagtt taaagaatat caggtacaag ttgctaaaaa
    46261 tgcaaaagcc ttagctgaag aacttattaa aagagatttt aaattgattt ctggaggtac
    46321 agataatcat ttaatactat tagatttgac taataaaaat ataactggta aagcagcaga
    46381 aaagagattg gatgatgctt atataacagc taataaaaat acaattccat ttgacccaaa
    46441 tggtgctctt gttacaagtg gaataagact tggtactcca gctgtaacta ctagaggtat
    46501 gaaagaagaa gatatggcaa taatcgctga ggctatagac ctatgtttaa cttatgatga
    46561 agaatctaag gctagaacat tggttgttgg tttaactgaa aaatatcctt tatatgaaga
    46621 atataatatt atggattaat tataaagaac aactaaataa aatacattta aataattaca
    46681 atgattgtgt atgtatttga atatatttag atataaaaaa ggtgtctcaa aatgaacttt
    46741 ttagtttatg aggcactttt ttgtatggaa tcgaacatat ttgtattatt taaataataa
    46801 aaaatatggt tatctctatt tttagatggt ctctttgtat tttttaatta aaactttaag
    46861 atttcttaag aattatacta ggtttttcta aagatttaaa gtctatactt ataagtatag
    46921 taatcatatg aaaagaaaag ctttaacgag gagggaatct tatgaaggta ttaattctta
    46981 caggaaaatt tggtatgggt cattactcag catcaaattc tttaagtgaa gatataaaag
    47041 caaaatttga taattcagaa ataataatta aagatatatt tgagtacatt atgcctaact
    47101 attcagataa aatgtacaag actttttcta ttcttgtaaa tcggggaagt agtctttata
    47161 atttatttta taaatgtgct gaaaatggaa agaaagatat aaaatttact ttttcagatt
    47221 attttttaaa taaattagat acattacttc atgaagtaca accaacagta gttatttcaa
    47281 cttttccatt ttgttctcaa ttagtatcaa gatataagga aaaatataac tcaaatcttc
    47341 ctttaataac atgtataact gatataagta gtcactcaga atggattagt aaaaatacag
    47401 actgttattt agtagctagt aaatctacta aggaagaact tgtttttaaa ggaatagatg
    47461 aatctaagat taaagttaat ggtattccag taaaaaaaga atttaaaaga atagaacatg
    47521 taaatcattc aactaaaaaa aatattctta taatgggtgg aggattagga ttactgccta
    47581 aatcagaaca attttacaaa gaattaaata gtttagaagg agtaaaaact actgtaataa
    47641 ctggtaataa taaaaaaatg tactataaac tatatggtag atatgaaaat atagaggtag
    47701 taggttacac caatgaagta tataaatata tgaaagattc tgaccttatt atttctaaac
    47761 caggtggaat aacactattt gaaacaattt attcagaact tccaatattg gcatttaatc
    47821 catttttaca acaagagata gataatgcta gttttatatt aaataatgaa attgggcgaa
    47881 tacttgataa aaataaaaaa tactatgtag atgagataaa agatttaatt tatgatgatg
    47941 ctactcttaa agaaatgagc agtaacatga aagaattaaa aaaacaattt gataataata
    48001 ccttagagaa tattttattt tcacttgatg aacaaggagc gtgtagagaa tgtatgtagt
    48061 aggtttaatt atcgttgttg cattgatttt tttagtccat tcaattatac ctacatacta
    48121 taataaacta ttaaataaag aggtattaaa aaatatggct ggagaaaatg aaattgcact
    48181 tacatttgat gatggtccag ataagagata tacagaaaaa ttactagatg tgttaaaaga
    48241 aaatgatata caagctatgt tttttgtagt agcaaagaat gctgaaaaag agcctgaaat
    48301 tataaacaga atgttgaaag aaaatcatat agttggttta cattcattgg aacacagaaa
    48361 tgcatggctt tatagttata gttatgttaa aaaagatttt attgaaagta caaatataat
    48421 gaaaaattta ggtgtagatg taaactacta tcgtccacca tggggtcata ctaatatatt
    48481 ttcaaattca tttgttaaaa aatataatct aaaaatgact ttgtgggatg taatggcaga
    48541 agattgggag aaagatagta cagtagacat tattataaac aagttaatga gtagaacaaa
    48601 agaaaattct ataatttgtt tacatgatgc aggtgaaaat tcaggtggtg cagtaggtgc
    48661 acctgagcgt actattgaag ctttaaagat agctattcca aaacttaaag ccagtggttt
    48721 aaagttcgta acaccagaaa ggatgtagtt atgataagta aaactgaaaa gaaaaataaa
    48781 tgcctaggta gtgtagcttt tttagtttta ctaatgggca taacaggata ttttgttttt
    48841 agagggcagt cagttgaatc attgataaaa tcattaaaag gtgcaagccc tatgtttata
    48901 ctaattggat ttgcaatgat gtttatatat gttgcctgtg aaggtataaa tatatactta
    48961 ggtatgaaag ctttaaacca aaagactact cttttaaaat gtatgggata tgcttttatt
    49021 ggattttatt ttagttctat tacaccttca gcatcaggtg gtcaaccagc acaagtttat
    49081 tatatgaaaa aagacgatat aaatatatca tattcttcac ttatactatt agtcattgtt
    49141 gttattcacc aagtagttat actggcatat agtggaataa tgttcattat ggaacgtgag
    49201 tttatattaa ataatgtaag tggtatgaac attttactaa tatatggtgt aataacaaat
    49261 gttgctcttg tcattggagt aattgcaatt atattttcaa aaaaacttgt aaacaatttt
    49321 ataatatcaa taacaaattt attaggtaag ttaagaataa taaaagatgt tgaaagttca
    49381 agaaaagtta taatatctca aattgaagag tatgttaaag gtgctcaata tataaagcag
    49441 aatccaaaac ttgtagtcca aattttagtc ataactattg tacaaataac agctatgttt
    49501 ttagttcctt tctttgtata caaagctttt catctatcaa catatactgt atttgagata
    49561 ttagcgatac aatccttgct taatattgct gtttcttcat taccattgcc aggggctgtt
    49621 ggagcttctg aaaatagttt tatgacatta tttaagatat ttttccctgg tcacttatta
    49681 gttccggcaa tgttacttag tagggggata agtttttatg catttgtagc tataagtgga
    49741 ttgatatgta tagtagtaca tgtaaaatct tctgaaaaag taaaagcagt gaaaaaagtt
    49801 gcttatgtaa gataatatta tatagattat ttttattaat atgtcaattt gtgtgtatat
    49861 tttaattaag ctttaaacat gtttacagga gtagaaacta ttaaaacgtg tgacattgct
    49921 aagcttttgc taataaagta taatatttta tatagtttga aatatttaag aaatatagca
    49981 aaaaaaggag cttggtgatg aataaagaga ttccaaaaaa aattttagta gctgatgata
    50041 atcctgagat tagggaaatc gtagaaatac ttttaactgg tgaaggttat gaagttatta
    50101 tggcaacgaa tggagaagaa gctgtaaact tagtagatag tacgattgat ttgattattt
    50161 tggatgttgt aatgcctgta aaaacaggat ttgttgcatg taatgaaatt agagaaaaaa
    50221 cgacagcacc aatattattt ttaactgcaa aaactcaaga ctctgataaa gttattggat
    50281 tttcagcagg aggagatgac tatttatcaa agcctttttc atattctgaa ctaatttcaa
    50341 gggttaagtc tctacttaga cgttattata tataccaggg aaaggaaaag gaaagagagc
    50401 caaatatatt aaaaacaaaa gagttatgtg tcaatttaga tactcaagat gttacactaa
    50461 atggagaacc tgtgtttttg acaactatag aatatagtat attaattctt atgttgaaaa
    50521 atagaaaaaa agttttttcg tctgaaaatt tatatgaaac tatttgggat gagcagtatt
    50581 tttatacagc aaataataca ataatggttc atattagaaa tttaagaaaa aagttagaaa
    50641 aagaccaaaa aaatccacaa tatataaaaa ccgcatgggg aaaggggtat tatattgatt
    50701 aacaaaatta agccatctaa gttgggagtt atactattga tatatgtagt aatctcagct
    50761 gcagtggctt tcattttaaa tataccattt tactctgctg ttgaatctat agtaaaagat
    50821 gtatcagaag ggaaagtata ttttaaaaga gaagtaaatc aacttatgga taattttcaa
    50881 acttatgtga ataacaatgg tataagagca gatgatggag atgatatagc aaaatggaat
    50941 agtgataatt ggtttgtttc tatgaagata tataaaggaa ataaggtttt ttatgataca
    51001 ttatactatc ctaatgaagt acctgataag agtgaggaaa atgagtttta tagcagtccc
    51061 atggctagaa actatgagat acaattttct gatattaaag ctaatatagt tgtagtagct
    51121 ttatttagat ttagatttca aaattttata gaagcaattt ctattacatt tatggcactt
    51181 acatttatta taacattttt actgttgttt agtagaaaga taaagtacct aaggaaaata
    51241 gaaatgggta taaagatagt agagagtggc agtttaaagt atagaatccc agtaaaaggt
    51301 aatgatgaat tatcatcact tgcatctagt attaatgaga tgagtcatac attggagaat
    51361 caacttataa aagaaaggga gatgaagaat aaaaataaag atatagtaac ttcaatatca
    51421 catgatgtta gaactccatt aacgtcagta atttgttatt tagatttgat taaagaccat
    51481 aaatacaata gtcctgagca attggaacaa tacataaaaa atgtaaggtc taaagcatat
    51541 caaataaaag atttaacaga tgaccttttt acgcattttg ttaattctgg agaggaagtt
    51601 tcaaaagggt atgaaaaaat aaatgctaat gaatttattt ttcaattact tttagatagt
    51661 tcatatacat tggaggagaa aggatttcat ataacaatag aaaatgaact ttataaccaa
    51721 tatcatattt atgtagatgt tacacaattt agaaggatat ttgataattt atgttctaat
    51781 ataataaaat atgctaggaa agataagcct atagtatttt taattaaagt agaggattct
    51841 aaattactaa ttatacaaaa aaatagagta agtaattttt ctaagaatgt agaaagtttt
    51901 ggaattggta taaagaattg tgagaaaata gttgcatatc ataatggaag cataaatgta
    51961 aattcagatg ataaagaatt tgctattgaa atatctattc cagcaaaaga atcataaaaa
    52021 tttaaattat atatagtgta attgcttatg taaaattcta tgaagcagga agcatgtgac
    52081 tttagttatg ggatgttcaa tattgtgtac ctctaggcaa gtaacaagtt tacttacaag
    52141 accatataat attattatct taaaaaaagg ggacataata atatggtttt gacaggagca
    52201 cattatattt acattctatt tatggtaatt atattaatta ctatgattat gaaaaaggat
    52261 acaatagtac catgtatatt aggtgttttt tttatgggat tgttctttga aaaaaacatc
    52321 tttggtgcta taacagcagt gtttaactct tttattatat ctttaaatga gttgggtcca
    52381 attatcttga ttatagctat tatggttgca ttatctaaag ccctagaagc caataatgct
    52441 atacagtata tggtgagacc attttcgaga gttataaaaa attctaatac agcatttttt
    52501 gtaactggat ttgttatgtt agttctttct tggtttttct ggccaactcc agctgttgcc
    52561 ttagttggag ctgtattttt acctgttgct atgagagctg ggctaccagc aattggtgtt
    52621 gcagtagcat taaatttatt tggtcatggt cttgccttgt ctacagactt tgttattcaa
    52681 ggagctccaa gtataacagc tggtgcagca ggtgttgcag tatcatatgt tataaatgat
    52741 ggtatgattt tgttttgggt gatgggaatc gtaaccatat ctatggcatt ttatacatta
    52801 aaaagagata ttaacaaagg aatgtttaga gaagaactaa aaggttttga aagtgaagaa
    52861 gtagaagagt tcaatggaaa atctaaaata gcaactattt tagtagctct gggatttctt
    52921 gctgatataa ttgctatgta tatatttgac ctaaaaggag gagatgcatc agctcttttg
    52981 ggtggaacag cagtattttt aataataatt ataaatgtta taaattttgg caaagatagt
    53041 cttgaaaatg tatgtgagaa tataattgat ggttttgtgt ttgggataaa aatatttgga
    53101 gcaattatac caatagcagc atttttctat atgggtgaag ttgcaccatt gacaggtgta
    53161 tttggaaaag tattagcacc aggttcacaa ggtttattat cagatatagg catagctctt
    53221 tctcaaacaa taccacttaa taaatttgcc gtatcaggta tagaaactgt agtgggagca
    53281 ataactgggc ttgatggttc tggattttct gggatttcat tagttggttc acttgcctct
    53341 gtatttggaa ctgctataaa tgcaagtgtt ggtgcactag ctgcattagg acaaatcagt
    53401 ggtatatggg taggtggagg ctgtctagtt ccttggggtc ttatttcagc agcggcaatt
    53461 tgtggagtta gcccaataga attggcaaag cgaaactttg tcccagttat aaccggattg
    53521 gttgtaacaa ccatagtagc aatgtttatt atttaataaa tcgaataaat ctttaattgt
    53581 ataaattaaa aatagatgtg tagctataat ttattataat tgttttacta attctaagcc
    53641 atacatctat ttttattgat atttttatta gtattcttta ataaaataat atttttcatt
    53701 atactataca taacatctaa atataacatc taaaatactg ttatctatat ttaatatttt
    53761 gccaaatttt ttgatgtttt attgccctgt cccaccaaca tatataagct atcacaagtt
    53821 tttacctata ctatcaccaa acaaaaattc taattttaat ttattatttt ataacctata
    53881 ttatattaac tatattagtt tgtagacttt agatgtaatt ataaatatta atactccaag
    53941 agttcaaatt catatcctag ttttcttgca tttgatataa aatcatttaa aatttctgta
    54001 ctagttttgg aaactgcatg tagtaacaag atagaaccat catgtagatt atccattatt
    54061 ttttgcgttg cttcttcatg tgatggttgt ttatcagtat cccagtctct gtatgcaaaa
    54121 ctccaaaata cagtcttgta tcctaaattt ttagtcattg ccaagctttt ttctgagtat
    54181 tttcccatag gaggtctaaa aaatttaacc atatctttcc cagttacatc tttatataac
    54241 ttttcaacat catataattc gtcattaaag ttttttagat tgcttgtttt tgttggcata
    54301 gatggatggg ttttactatg atttccaact atatgtccct cagaaaccat tctttttact
    54361 aaatctttat tttcttttat atatggagct gtaacaaaaa aaactgcttt aacctgattt
    54421 tgttttagag tgtcgagtat ttttgtagtg tatccatttt cataaccttc atcaaatgta
    54481 agatatagtg ttttttgacc atcttttgta ggtccattgt atagggcatc atagtcagaa
    54541 aatttaaatt caatgtccgt atttacttct ggtgttttat gcttattgtt tggtatataa
    54601 aaccaatcaa gagtagtgtt atctaaagaa gatacatcta atttattatt atcattatta
    54661 gtagtggctt ttgatggttt atctgttccg tctgaaacta ttacattgga agtatcttta
    54721 ccactatcta ttttttcctt atcttcttgt aactgtgttt ccttattttg attttcgttt
    54781 tgattgtttt gtgaatttga acatccaaca ataaaaaaaa tagaaataga aagtagtgtt
    54841 aaataatgca atacttttct aaacattttg aagctccttt ctaaattaat aataattgca
    54901 tataataata attatatgat taattacaat tgcatatagt attattataa ttggaaatta
    54961 ctattaataa tagttatatc tttaaaaata aagtcacaat agtatgacaa agggtgaata
    55021 atcaataaat ttaaaaaaaa atgaaaaaaa tagttgacca tattatgaga agatgttatt
    55081 ataataaatg tgctcaacaa gaagtaacaa aataacaagc acaaagaagt taattgaat
//
LOCUS       DAEMHZ010000008        53920 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000008 DAEMHZ010000000
VERSION     DAEMHZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.008"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(464..2068)
                     /locus_tag="KPK72_000443"
     CDS             complement(464..2068)
                     /locus_tag="KPK72_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2487688.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2454..3380
                     /locus_tag="KPK72_000444"
     CDS             2454..3380
                     /locus_tag="KPK72_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2487689.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3500..4498)
                     /locus_tag="KPK72_000445"
     CDS             complement(3500..4498)
                     /locus_tag="KPK72_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG2487690.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4602..5039
                     /locus_tag="KPK72_000446"
     CDS             4602..5039
                     /locus_tag="KPK72_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2487691.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5125..5712
                     /locus_tag="KPK72_000447"
     CDS             5125..5712
                     /locus_tag="KPK72_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2487692.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6012..6632)
                     /locus_tag="KPK72_000448"
     CDS             complement(6012..6632)
                     /locus_tag="KPK72_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG2487693.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6640..7407)
                     /locus_tag="KPK72_000449"
     CDS             complement(6640..7407)
                     /locus_tag="KPK72_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG2487694.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7448..8269)
                     /locus_tag="KPK72_000450"
     CDS             complement(7448..8269)
                     /locus_tag="KPK72_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487695.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8628..10538)
                     /gene="phnW"
                     /locus_tag="KPK72_000451"
     CDS             complement(8628..10538)
                     /gene="phnW"
                     /locus_tag="KPK72_000451"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG2487696.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10817..11602)
                     /locus_tag="KPK72_000452"
     CDS             complement(10817..11602)
                     /locus_tag="KPK72_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2487697.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11819..12049)
                     /gene="dltC"
                     /locus_tag="KPK72_000453"
     CDS             complement(11819..12049)
                     /gene="dltC"
                     /locus_tag="KPK72_000453"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG2487698.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12068..13216)
                     /gene="dltB"
                     /locus_tag="KPK72_000454"
     CDS             complement(12068..13216)
                     /gene="dltB"
                     /locus_tag="KPK72_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG2487699.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13216..14730)
                     /locus_tag="KPK72_000455"
     CDS             complement(13216..14730)
                     /locus_tag="KPK72_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG2487700.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14851..16041)
                     /gene="dltD"
                     /locus_tag="KPK72_000456"
     CDS             complement(14851..16041)
                     /gene="dltD"
                     /locus_tag="KPK72_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG2487701.1"
                     /translation="MRKLIYFITPFIIGIVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16329..17348)
                     /locus_tag="KPK72_000457"
     CDS             complement(16329..17348)
                     /locus_tag="KPK72_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2487702.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17524..18063)
                     /locus_tag="KPK72_000458"
     CDS             complement(17524..18063)
                     /locus_tag="KPK72_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG2487703.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18164..18844)
                     /locus_tag="KPK72_000459"
     CDS             complement(18164..18844)
                     /locus_tag="KPK72_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG2487704.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18873..20162)
                     /locus_tag="KPK72_000460"
     CDS             complement(18873..20162)
                     /locus_tag="KPK72_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2487705.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(20626..22227)
                     /locus_tag="KPK72_000461"
     CDS             complement(20626..22227)
                     /locus_tag="KPK72_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2487706.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELDINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(22338..23015)
                     /locus_tag="KPK72_000462"
     CDS             complement(22338..23015)
                     /locus_tag="KPK72_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487707.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(23032..23727)
                     /locus_tag="KPK72_000463"
     CDS             complement(23032..23727)
                     /locus_tag="KPK72_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG2487708.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(23779..24978)
                     /locus_tag="KPK72_000464"
     CDS             complement(23779..24978)
                     /locus_tag="KPK72_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2487709.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(25239..27284)
                     /locus_tag="KPK72_000465"
     CDS             complement(25239..27284)
                     /locus_tag="KPK72_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2487710.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(27695..28504)
                     /locus_tag="KPK72_000466"
     CDS             complement(27695..28504)
                     /locus_tag="KPK72_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2487711.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(28588..29121)
                     /locus_tag="KPK72_000467"
     CDS             complement(28588..29121)
                     /locus_tag="KPK72_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2487712.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(29271..30620)
                     /locus_tag="KPK72_000468"
     CDS             complement(29271..30620)
                     /locus_tag="KPK72_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2487713.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGADLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(30632..32014)
                     /locus_tag="KPK72_000469"
     CDS             complement(30632..32014)
                     /locus_tag="KPK72_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2487714.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            32258..33472
                     /locus_tag="KPK72_000470"
     CDS             32258..33472
                     /locus_tag="KPK72_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2487715.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVNFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            33649..35562
                     /locus_tag="KPK72_000471"
     CDS             33649..35562
                     /locus_tag="KPK72_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2487716.1"
                     /translation="MKYGGIIMKEILVIAPTKETYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            35833..36807
                     /locus_tag="KPK72_000472"
     CDS             35833..36807
                     /locus_tag="KPK72_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2487717.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            36828..37103
                     /locus_tag="KPK72_000473"
     CDS             36828..37103
                     /locus_tag="KPK72_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2487718.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            37116..38279
                     /locus_tag="KPK72_000474"
     CDS             37116..38279
                     /locus_tag="KPK72_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2487719.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            38616..40895
                     /locus_tag="KPK72_000475"
     CDS             38616..40895
                     /locus_tag="KPK72_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2487720.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFTNQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(40990..42330)
                     /locus_tag="KPK72_000476"
     CDS             complement(40990..42330)
                     /locus_tag="KPK72_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2487721.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(42342..43124)
                     /locus_tag="KPK72_000477"
     CDS             complement(42342..43124)
                     /locus_tag="KPK72_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487722.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGDDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(43139..44161)
                     /locus_tag="KPK72_000478"
     CDS             complement(43139..44161)
                     /locus_tag="KPK72_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2487723.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(44167..45180)
                     /locus_tag="KPK72_000479"
     CDS             complement(44167..45180)
                     /locus_tag="KPK72_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2487724.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(45308..46246)
                     /locus_tag="KPK72_000480"
     CDS             complement(45308..46246)
                     /locus_tag="KPK72_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2487725.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            46516..46980
                     /locus_tag="KPK72_000481"
     CDS             46516..46980
                     /locus_tag="KPK72_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487726.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQSKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(47081..47398)
                     /locus_tag="KPK72_000482"
     CDS             complement(47081..47398)
                     /locus_tag="KPK72_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2487727.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(47411..48142)
                     /gene="chbG"
                     /locus_tag="KPK72_000483"
     CDS             complement(47411..48142)
                     /gene="chbG"
                     /locus_tag="KPK72_000483"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG2487728.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(48168..49478)
                     /locus_tag="KPK72_000484"
     CDS             complement(48168..49478)
                     /locus_tag="KPK72_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2487729.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(49575..50888)
                     /locus_tag="KPK72_000485"
     CDS             complement(49575..50888)
                     /locus_tag="KPK72_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2487730.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(50910..51221)
                     /locus_tag="KPK72_000486"
     CDS             complement(50910..51221)
                     /locus_tag="KPK72_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2487731.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGSQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(51421..52125)
                     /locus_tag="KPK72_000487"
     CDS             complement(51421..52125)
                     /locus_tag="KPK72_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487732.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(52216..53577)
                     /locus_tag="KPK72_000488"
     CDS             complement(52216..53577)
                     /locus_tag="KPK72_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2487733.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVAIGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
BASE COUNT        18201 a         9238 c         6164 g        20317 t
ORIGIN      
        1 ttttttaaat tagaattatt ttgatgaatg attcaagtaa taggtatgaa attgaatctt
       61 ttagaactgc ttacaaaaaa tactcaaaga aaaatagtaa ttttctgaga ttcacaatat
      121 attttatatg aaatgttata aggtaatcta tttaataaat ttaaatttgc tattatagat
      181 ttagttccaa agtcttactg caaagtaata tacatagttt tttaataaac taataagttc
      241 aattaataac tacttaattg tttaatatag tacatagata actttaataa atcgacagtt
      301 atttaaaaag gtagaatcaa taaaattata cttataaatc ttcaatacaa taattaaaat
      361 ttaataaaaa tataattgtt gatttaactg taatcttatt taattctacc taacttatta
      421 tataaaacta ttttttatag attataaaat atctaacttt catctacttc aaagaataca
      481 atacttcccc ttctcttata gtctgcaata ctttaatatc ttttattttc atagggtcga
      541 ttgtaagtgg gttttcatct aatattatca aattagctaa tttcccttct ttaattgaac
      601 cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc tttaaagcat
      661 ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta attctattga
      721 cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc tgatgaagag
      781 tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac gctctatctt
      841 ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca aagtatgaag
      901 gtatcatatt tattactttc atatcatcaa tctggtctgc tcttactgtt tgagcatgta
      961 tcataactgg tcttatatga ttttcactac tttgctcttt taagtttaaa actttctcaa
     1021 acgcatctat caactggtct gctgctgcat ctccattaca atgagttaag agttgcatct
     1081 tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat ataggatatc
     1141 cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc ttaccttgtg
     1201 gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata tattttttta
     1261 taaactccct attattatct actacagact tacttttttt caaatttaca tatcctacaa
     1321 catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat tgattttctt
     1381 ccattaaacc ttcttgagct gttgtaatac catagcttag atatatgttc tgtgcttttt
     1441 caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct acattaaaaa
     1501 atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta cctatatgtc
     1561 caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc aatgtatttg
     1621 caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa gatgcactat
     1681 cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca tatgaaaatc
     1741 caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt atttttttta
     1801 ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta gtagcaaaag
     1861 ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct tgtaaatcta
     1921 taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct atctttttta
     1981 ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt gtgatgatat
     2041 ttccattgta ataaattttt gctttcataa aattctcctt acgttgtcta cttttaataa
     2101 atttttttca ttattttctg cttaattata ttatcttctt tataaacttt ttctttagag
     2161 tttaatcata taaaagtttt atttttaatt attgtttgcc aagaagtaaa tactattcat
     2221 tgtaaataag acaaattaat aggctttata ctaattgaaa aaatattata agttgttaaa
     2281 tttaaatatc taaactcaat tatatctgta caattgtaat tttttaaatc atgatgtatt
     2341 attttaaata tacagttaca ttattattca caatataagt atcttagttc tcaaataaca
     2401 tctcatgtaa tctattcaat gcaaaaaatt taaatatttt ttaggaggtt tttatgagca
     2461 aggaaaataa aaatgtaaga attgctgttg tccaggcgtc accagtcata atggatttag
     2521 aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc tggtagaaaa ggggcaaata
     2581 tcgtagtttt ccctgaagca ttcatacctg catatccaag aggtctttca tttggatttg
     2641 ttgttggaag tagaacaatg gaaggtagag aagattggaa aagatactat gataattctg
     2701 taccagttcc tagtgctaca acagatttat taggaaaagc agcacaagaa gcaggagtat
     2761 atctatcaat gggtataaca gaaagagatg gtaatgatat aaattgtacc ctttattgca
     2821 caaacttatt tttctctcca gaaggtaagc taattggtaa acacagaaaa cttaaaccta
     2881 caggaacaga aagatgtatt tggggagaag gagatggaag tactcttaca gtagttgata
     2941 ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa ttatatgcca ttagctagaa
     3001 cagctcttta tgctaagggt gtaaaacttt acatagcccc tactgcagat tcaagagaag
     3061 agtggcaagc aactatgaag catattgctt tagaaggaag atgttttgta attggatgca
     3121 atcaatatgt tgaaaaaaat atgtatccaa ctgaccttaa ctactataag gagctagatg
     3181 cagaaccaga aattatgtgt ccaggaggaa gttgtatagt tgaccctttt ggaaaatatg
     3241 tagcaggtcc tattttcaat aaagaagaaa tgctaatagc tgatttagac ctagaaaaaa
     3301 tagtacttag tagactagat tttgattcag aaggtcacta ttcaagacca gatgtatttg
     3361 aattaattgt tcatgaataa tctttttaca taaacatcta cataaatctt tttacataaa
     3421 aaaatgagat actcataagt aaatttcaag tatctcattt ttgcttaaaa cctataatcc
     3481 taattataaa tttattcacc tatatttttt ctaaatcaac tactgttctt ccaacatgtt
     3541 ttcctgccat tattgaatct atactatcat ttaactcttc caatgatact acatttacca
     3601 tatcatctaa atcatctaat ttccattcat ttgataataa atcccatatt tttaatctta
     3661 agttcattgg gcaatccaca gaatctatac catataaagt aattcccttt aagataaatg
     3721 gatatactga acttgtaaaa tcaactcctc ctgcattacc acaagttgtt actgctccac
     3781 aataattagt tgtctttatt gctgttgcta gagtatttcc tcctactgta tctattactc
     3841 cagcccatct tggtctaagc agaggtcttc ctgagttatc atccacttct gacctatgta
     3901 taacatcttt aacacctaag tttagtaata cttctttttg ttcttctaat tttcctgttg
     3961 aaactacaac tgaataacct aatttaatca atattttagt tgcaattaca gcaactcctc
     4021 cagtaccacc agtgactaat acatctccca tattcttgtc tacagaatct ataaatttat
     4081 acacagataa agctgctgta aaccctgctg taccataatc cattgcttcc tttagtgaaa
     4141 gatttttagg aagtttaact acccaactag aaggaactct tatgtattct gaaaaaccac
     4201 cagaagtatt cattcccaag tcataacctg ttactaaaac ttcttctcca attttaaaat
     4261 catcacaact actagattct actataccag atgcatcaat tcctggtgta tgaggatact
     4321 ttctcgtaac acccttatta ccatttgcag aaagtgcatc cttgtaattt aaagaagaat
     4381 attttacttt tattagaact tcgccttctg gcaatgtatc cacttctctt tcaactatat
     4441 ttcttctaaa atttccttct tcttctgtaa tatatagtga tttaaatttt ttattcataa
     4501 atttcctccc aatacattta gttttatgtt aaactaatta tagtttataa atagttttat
     4561 gtcaaactaa tttaaataga ttaaagattg gagaataatt tatggaagat aaatatattg
     4621 tacatttcat aagtaaaaca aaggctagta tgattaaatt cattgaaaat aaacttagta
     4681 aaaatggttt aggtgagtta atacctactc atggaaatat tttaactgca ctttatgaaa
     4741 ataatggaat acttacaatg aaagaaattg cagctaaaat aggaaaagat aaatccactg
     4801 ttacagtgtt ggttaataaa cttataaacc taggatattt agaaagacaa aaatgtacaa
     4861 atgacaaacg tatcacgtat ataaagctta caaaaaaggc actgcttata gaagatactt
     4921 ttaatagcat ctcaacacaa gttaaagaga cagcatacca taatattaca gaaaatgaaa
     4981 aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa ttttaaaaat gctgactaaa
     5041 ttgagcataa ttatatctcc tattggaata ataaataaaa agaatcaaat attgtaaaaa
     5101 tttattgtga tagagaggta gaatatgaat ttaaaagata gtaaaactaa agaaaattta
     5161 ttaagagctt ttgcaggtga gagtcaagct agaaatagat acaatatttc tgcatctgta
     5221 gcaaaaaaag aaggattata tataatagag tctttattta attacactgc tgaccaagaa
     5281 aaatcacatg caaaacaatt cttttctaaa ttaaaagaat ttgctggtga aaatataagt
     5341 atatcaggag cataccctgt agatttatat gacaagacag cacaacacct taaagctgca
     5401 caacataatg agcttcaaga gtgggatgat gtatataaaa actttggaaa aacagcacgt
     5461 gaagaaggat ttgaatctat agctaagtta tttgaaaata tagctgaaat agaaaaagtt
     5521 catggtgata gatttggtag atatgctact gatttagaag aaggtacttt attcagaaga
     5581 aatgaagata tacaatggat atgtacacat tgtggccata tacatatagg taaagaagct
     5641 ccaaaagctt gtcctgtatg tcttcatcca caaggatatt tcttagattt taaaaactca
     5701 ttatttgaat aaattaaggc aataattcta tttaaacaat ttactatatt tatatcaagt
     5761 atttttataa atactgtatt atcttaataa caataaaatg aaccattgat attactataa
     5821 gtagaaaatg tagtttaaat acaatttata gtaattcatc atggttcatt ttattattta
     5881 ctaatcccta taacctactt aaatctatct ttaactttta catactcaca taaaatctta
     5941 tctttaaact aaatatttaa ataaagtgta ttttaagtat attattataa tacattatat
     6001 aagataatac actatgataa ctttatactt atatcattta tttcttttgt caagtcaatc
     6061 tcaataatct taactacatc tttttcaaac tttacttttt tataatccag acatcctact
     6121 acgaatggaa ctccaaactt tctaaacaaa taaatctttc cactttcatt gtatattgat
     6181 gtaaaatttt cttctattat attcactatc aattttaact cttcaaaagt tattatagat
     6241 ttaactaaaa acttctctat tatgctcaat ataagcagtt cttttttacc tatgctttgc
     6301 tcttttttat ataaattttg aaatatttct ttggtataat tacatataac ttctttactg
     6361 agaataaaat caatgtcaaa ttctttatta atatcctttt tcataaacat atttgcaata
     6421 tcatcaagag aagtctcttc tttcatagac aatattaact ctattctttc tagtatttta
     6481 tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa ccattcctct
     6541 ggtattatgt tttttctttt ccatctatat aattgaccat aagatatatt agttttactc
     6601 aataaatctt tcttagatat taattcttcc ataacttttc tagcccacct ttatactgtt
     6661 ttttacagta gaattatctg aaactgataa attatcactg tattcaatat ttccaacgtc
     6721 acaattatct actagagata tgcttttacc tcgtatatct tttattgatg catcttctaa
     6781 ttcaatagta tctccctcta tcaaattaca tttaaacttt cctgaaaaaa tattaatgat
     6841 tttaattaat gaaaaagcat ctttgcttct tcctattttt atattagttg cccctatctc
     6901 tttacaataa gaacctcctc ctgaataaac atatatattt tctccattta aaagaccatc
     6961 acattttatt actccatata cctttatatc ttcacattct atatttgatt tatttctcat
     7021 ttcgccatag atttttatat cttgtgcata catattttca tttacactta attgtcctct
     7081 tattgtaaca ttattacatt taagtttgcc atccactttc tgttgagcag ttataactaa
     7141 gtcatctatt tctgcatctc cctctatatt aagttgacca ttcactctta cacgctctgc
     7201 tttaagattg cctcttataa tagtttttcc gttaacaaca aaatttttta tattgatgtc
     7261 accttcaaat tttgcatctc caaggacttt tacttcgtta cttttaacat ttccttttga
     7321 tttagaactt cccacgattt ttatattttc atactcacca ctacttataa agccttctcc
     7381 aagtattttt atagaccctt tttccatatt attacctcat tttaaatttt atttttaaac
     7441 ctatttatta cttatccttc tttaatttta ttttttcact tatattcttt atttcaccaa
     7501 ctattgaatc ttcatcaatc tctatacttt cactgtactc caaactttct acataacatc
     7561 ccttgcctat tttaatgttt tttcctctta caactttgac ttcactattt tctaagctta
     7621 tttcatctgc ttctataata tttgcaaaca atttactttc tgtatacttt ctaattgata
     7681 atagacctaa acttattaaa tttgaagaca ctttttcaaa gttgatatgc tttttttcta
     7741 gcatacgaaa ttttttgcaa taatctccat gccccttttt tatatttata ctacttgctc
     7801 caacttcatt aaaattagaa gttccattta aactaatatt aacttcttca caattcaaaa
     7861 atccattaca ctccaagatt ccacttaaat tgatattttc acaatctata ttctcatttg
     7921 cttttaaata tccttctcca tgaataatat ccgattttat acttccttct acattaataa
     7981 ttccactagt atcaatttca acagaattaa tatttcctgt acatctaaga agtcccgata
     8041 tctcaacttt tgatgccagt atgtttcctt ctactttact taaaccagat gtaaaaaatt
     8101 cattacattc tgttgtccca aaaacttttg ctgaaccaga agaatcaaat ttactacatt
     8161 ttacacttcc ttctattttg cctgttcctg atatattaac attatcaaaa attccacctt
     8221 tacattttcc aacaccatca aatcttagtg aacttaattc ttcattcata atgatatcct
     8281 ttctttaccg tattttatct ttaaacctaa tataacaaaa cattgttacg ttgtcaagtg
     8341 tgataataat acttaataag gcaactatca tttattttct ataaatgggc ataaaaataa
     8401 tagggaaatg ctttatttaa actataaatc taatttctta gattgtggtt taaataaaat
     8461 atatccctat tactcaatgt ttatatattc attgtataaa cttctaaaat ataagtcttt
     8521 aaattctact gtcaatactt tttatacact gtttgtgcta tcatctcaca cacctaaagg
     8581 agtaggcttt gcgcactatt ctgtaaccta caagacacta tcttcttcta tctattgata
     8641 aatttttcta ttacatcagc tagtctctcc atatcagttg gataaacttc acctatgcta
     8701 cctattctaa atgtatctat atcagtcaat ttacctggat agataacaaa tccattatct
     8761 tttaaataag tataaaattc cataaattca aactttgcat ttttaggata taagaaagtt
     8821 gttattacag gtgattgagc attatcattc accaaagtat caaatcctaa agattttaat
     8881 cttgaagcta ttgtaaactg attttcttta tatctagcat atcttttttc aacagaacct
     8941 tcttcttcca attctaataa agcttgataa aaagctctta caacatgtgt aggtgaagta
     9001 aatctccatt taccattatt tttctccata gtctcccatt gcgcatacac atcaagagat
     9061 aatgatttag ctataccttt acatttagat aattcctctt tattagctat tataaaacca
     9121 aatccaggta ctccttgtat acatttgtta gaactactaa ctaaaaaatc tatattaaaa
     9181 tcttcaacat caatctcaat tcccccaaaa ctactcatag catcaactat ataaattttg
     9241 ttatattctt ttgcaagttt ccctacctct tgtataggat ttaatctacc tgtagtagtt
     9301 tcacagtgaa ccattgatat atgtgttata tccttgtttt cttttaataa attttctact
     9361 acatttaaat ccactgcttc tatatcttta aaagtgcagt caacaaattg tatgttaaga
     9421 taattacata tatctttcat tctctttccg tatgctccat ttgcaattac aagaagttta
     9481 ccatcttttg aaatagtact tcctattata gcttctactg aaaaagtccc acttccttgc
     9541 attaatacac ttgtgtattt atctgtattt tgggttgcta gagatactaa tcttcttcta
     9601 acatcttgta ctaggttatt atattcaaca tcccaagtac accaatcttt taacatagat
     9661 gctcttactg atttagtagt agatagtggt cctggtgtaa gtaatatgta atcattttct
     9721 ggtacaaaat ctgatttaat agtttcattc tctattttta ttagtatatc ttctaactca
     9781 gccatagttt ctattacaaa atgtgctcct gcctctttaa atttctttga aactatactc
     9841 atcttagctt ttaattcttc cttatccatg ttctctactt cttcttgtgt aagccctaac
     9901 tcacttgaac cttttattac agcaacactc cacattcctg catttacgcc ttcttttaca
     9961 tcacttattg tatctcctac ttttaccata gaactcatag gagacacccc taaagcttca
    10021 gcatttttat agcacatcca aggatatggt cttccttgag atacttctga tggtgttaca
    10081 agaaaatcgg gcgagtatcc tttctttgct gcatttggtt caactatgtt catcatctct
    10141 cttgtataac ccgttgtaga acctattttt aatccatttt ttctcaactt ttcaatagtt
    10201 tcaacaacat gaggaattgg agtcgtatac tcttctaatg tctcaaataa cattggctca
    10261 aattcagcat aaagttcatt tacatcatct tcagttggca ctttaccaaa cttatctgac
    10321 caaatattct ttattctatc catttcacac atttctctta tatggtctat ttttaatttt
    10381 cccataggtt ttcttgcttc ttccattgtt acatctatac ctcttctttt aaatatctct
    10441 ataaatacat taagaggtgc aaaacatcca taatctactg ttgttccagc ccaatcgaat
    10501 actactgctt ttattttttc tccatatatc tttttcatat ttatagcctc catagattct
    10561 ttttagaatt tttgtgtttt taattgtttt tacttttaaa gtataacttt catatgttaa
    10621 ttatacatca tatttaggtt aaatatatat taaatttctg tttttagttg tatttagttg
    10681 tttttatttt gtatcatatt cccataaaat cctaagttac aatctaaaat ttactttcta
    10741 atcttaatct atgaactgac tatattttat gaatttagtt gtattgagca tagttgtatt
    10801 atataaaact aagggtttat tttgcttcta tccatgttat atttatctca tttaacttat
    10861 ccttccattc attcggaggt tctacatcac aaataacaaa atctaattcc tctaaatctg
    10921 ctatcttata catactagca attcctattt tagaactatc acataataca aaactttcct
    10981 ttgaattttt tatatatgct tttgacaaca atgccttatt ttggtctata ctactaattc
    11041 caaaactatc aagtatacct tcactagata taaaagcttt atctacgtat aagtttttca
    11101 tagtatctat tgatattgga catgctgtcc tttggtgttt taaatttact tttccaccaa
    11161 taaatataac ttctccatca aacaaacctt gattctcgta agaaatcaat tttgacagaa
    11221 ctggataaga gtttgtaata atttttaaat tgtttacaga tattatataa tctacaatct
    11281 gcaaattggt agtaccttca tctatgataa ttgactcatt gtcatttaca aacttagctg
    11341 catatttagc aatactttgc tttttatcta ggttagtatt gtttctcttt atatgttctg
    11401 gttcaatcac tccataattt atttttatag caccaccata tacctttttt aatttatttt
    11461 cattttctaa ttcttccaaa tatcttctta ttgtttctga tgatacttct aatcttttta
    11521 ctaattcttt agtgtttacc ttcccttttg aatctagtaa tttaagtatt tctttttttc
    11581 tatctatcga atttaaagac attcattttc ctcctaaata taatatattt ttctcctata
    11641 atattacaat gatacctaag atttatgaaa tcatatttat gtattaaatt ttttacttaa
    11701 ttaaagttat tttcaaaaga ctttgtatta tcattaaaaa agtccatcaa atattagtat
    11761 cagatggact ttgtaaaatt ttattttata aattaatatt taaagtctaa ctactaattt
    11821 attttcttgt ctccaagaat tttactaaat tatttacagt agacatatct tttctctcta
    11881 aatcagttgg ttgaagctct atccctaatc tattttcaat ttcaagtaaa acttcaatta
    11941 tagctaatga gtctaaaagt tccgtctcaa ataaattaag gtctaaatct tctcttatct
    12001 catcagtccc taatacatct tcaaatattt ctataactgt ttcttgcata aattttcctc
    12061 cttaaaatta agctaaataa cctgagaata tcagcaatcc aaaacacaca atatggaatg
    12121 taatgaaaat ctgcactctc tcgaaccatt tatctttttt atgtttttta taaaatttag
    12181 attttcgctg atatatatca gttaacacaa gtgccaatcc ttggtataca ccataagcaa
    12241 cataatacca agttagacca tgccaaaatc ccattgttat catggttatc atctgagcca
    12301 catgagcagc agtagtcctt ttcttaaatc ttttctttct cattgatgac ataacaaatc
    12361 tagaaaaaat ataatctcca aaccatcttg aaagacttat atgccatctt gtccagaact
    12421 ctttcatatc tttactgata aatggcttgt caaagtttat aggtacttgt attccaaata
    12481 tgtaccctgt acctatagca aataaactat atcctgcaaa atcaaagaac aggtataaac
    12541 tatatgcgta catataactt aaaatattta taaaactcat atctttaggt attcttgata
    12601 cccaatatgt atttatcaaa aatgctataa caaacttata acctactccc atgacaatat
    12661 tttttattcc tggcagtaag tattcttcta tatactcttt tctagagatt tgtttttcca
    12721 agtcctgttc aaatcttctt gacctatcaa taggtccaga acttaaagtt ggaaaaaata
    12781 acataaaata aagcatcttt gaaattttaa cttcttttat agctccatca taaatttcaa
    12841 taaccatttg tatagttctg aaatttaagt atgatattcc aataaatcca attattccaa
    12901 aagaagtaac tggagatatt ttatttatta ttattgggag cattgatgca aacaagaatc
    12961 cccaatatat gtatttattt ttagtttttc ttcttacata ttcatatcct ttaactatta
    13021 ttacttcaag aataataaat attaccaagt actttagttg gacatcaatt cctactatta
    13081 agaatatcat aaacaaactt gccaacatac cataatactt tatattcttg cccattagac
    13141 ctaaaattac tgctggtatt gatgttaata ataatatata aagataaaaa taatctccat
    13201 attgtgaaaa aatcattaaa tttcctccat aagtttcttt ctgtctattt taccattgat
    13261 atttgttgga aactcagaga ttattgaaat atttcttgga atcatatatg aaggtatata
    13321 ctttccaagt tcctttttta ttatcatccc atttttaata ttacttaaat cattcttttc
    13381 atttaactca acaatacctt ttaagtaagc aattttttcg tccttatata ctggtaaaac
    13441 tactgcattt tttacattat gtacttttct caaattattt tctatatctt ctatttcaat
    13501 tctaaatcca tttaatttaa tctgaaaatc ttttcttcca caataatata tatttccatc
    13561 taaaagatat cccatatctc cagttttata agctctccac tttacaccat ctatttcatc
    13621 atagaaaaat acttcatcag tcttttcttt attattgaag taacctttag aaactgatgg
    13681 tccaattatt ataatttcac ccttttcatt ttctttaagc tcatttccat cttcatcaag
    13741 tatttttatt ttacaatttg acatagggta tccaactgga agactttttt catcatctat
    13801 agccttttga gtcatatcat ttacacttac accaacagta gcctcggttg gtccatatcc
    13861 attgataatt cttgtgtttg gaaatctact cataagttct ttagtaagat tttttgaaag
    13921 tgcttctcct atgaatatca ttgactcaag gttaggaagc attttactat taaactcttt
    13981 ttcagttata catactcctg caaaagaagg agttgacacc catacagcta tatctgagat
    14041 actaaattgt ctaaatagtt ctttatagtc tgctaataca tcttttgata ttgaaaataa
    14101 tgtagctcca tggataagac cagggtatat tgttgtaact gaaaggtcaa aagaatatgc
    14161 tggttggttc attattactt tttcacttcc atctatgttt agatatgggc ttatccaatc
    14221 tgaaaagcta tctaaattgt ttgaacttat ttgaacccct tttggctttc ctgtacttcc
    14281 tgaagtaaat agtatatatg cattttcatc atcttttacc cagttttctt tatctaatga
    14341 ttttccctga tattcattta ttatataatt taatttatcc atatcaatta cattgatatc
    14401 tccaaaattt ctttcatcac taaagttaaa taataccttt ggttttatct ctttagtgac
    14461 ttcaaaaact ctatcaattg gaaaactaat atcaagaggc acatacgctc tacctgattt
    14521 taaagctcct atcatacatg ccattatcat gttttcttta tttccataaa ttactatagg
    14581 agtatcttct tctttgtata catcctttag aaatactgat atagcatctg aatattcatt
    14641 taaatcttta taagaaagtt tatctccatt acacattagt gctgttctat ctgtatttga
    14701 atattttttt attccttcaa taattttcat ataagaatgc tcctttaaaa tccgttataa
    14761 ataaatggca attctttaaa tggagtaaat gttagaactc ctatcgccaa tattataacc
    14821 atagttaaac aaatactagt taacaaatct ttattgttct ttaaatattt taaataatct
    14881 ctcacagaca tctacccatc ctttcgtacc aagatgcatt acatccctta aataatactt
    14941 atctgaccct ttatctttaa ggtttagaac ttcaaagcct ttgctttctg ctattttttg
    15001 tgctttatcg tagtactgat ttctttcttt ttcagaaata ccagtcaaat tatagaattt
    15061 atccatactt gggattaata caacaactgg cttaatacct aagtcagtac atacatttag
    15121 catcagttta taattttcaa actccttaga tgttaataaa tttacatctt tgtctcttcc
    15181 tttgtattgc tctataccat ctttaaaatg ctctttataa tagtacttat caatatttag
    15241 tggattttta ccaactcttt ttttagcatc ttcaatggct ttctttctct catatgacca
    15301 atttattggt ctttttcttt ttgttgctct ctttttatct agcttaatta atcgtttata
    15361 aagaataccc ttttctttaa gagctataca atactttctt ccttgaaaat aaggctctaa
    15421 aagcaccttt tcagcttttt caggaaatgt cttaggctca tatagcttag catataatgc
    15481 ttctgcttta tactcgtcac ttccccataa taacttactt gatttttttg catattcaat
    15541 cttattttgt ttagatattt ttggattatc taaaaatcga taaaattgta ttggtgaaaa
    15601 tctagactga taatggtgac ttgtgactcc gtctttttcc ataaaccatt gcatagaaat
    15661 taacaagaca actttcttat tatcaatatt aggattcata cttcctaata ttgcagcatc
    15721 ttgtaatgtc tgagtatagg ctctcccaat tgcaaacacc ttatttttac tcctatttgt
    15781 attaaaatag tactttggat gttgttttgt tgaattacta agttctgatg accccaaaat
    15841 cataatatcc ttttctctta aaaaatgatt atttgctgta acccctttat cttttatatc
    15901 acttaaagta tcatccataa ttggaagtag atttttctcc ctcaaaagtt catcagtttt
    15961 agaatcaaga aatttgtcta acccaaataa aaatactatg cctattataa aaggtgtaat
    16021 aaagtatatc aattttctca tattttctct cctctaaaaa tattcaaatt tatgttatta
    16081 ttaatcatat tagttttgat atgtatattc aactacaaaa ttgtaataaa atcgtcaaaa
    16141 ctttttttga tactgttaca aaattgtaaa aaaaaagaaa aaaacgccaa aaataaattt
    16201 ctttttagca ctttttcata cttataattt gacattttaa cacttggtta agtatacacc
    16261 ttaccttaac ttaaaattaa atttatttaa gaatatatat tataaattct tatattttct
    16321 aatataaact atatactatc agctggataa acaatactac tttgtcttct tatctcatcc
    16381 ataatttcca ttactattat tgaattttca tgagaattta tatttgattc tattttattg
    16441 ttatttataa gacttataaa ctctacaagt tcataataca ttccttctat ttctctaggc
    16501 ttttcctctg ctttttcttt tggaatagat atatcttctt ctcttccatc tctgtattta
    16561 ataatagctt tttcaaatag attaagcttt tcaattatta tacttccctc ttctccttgt
    16621 atttcagatg gtatataaga atcagctact ttagaatact gaattatcgc atccatatca
    16681 tcatattgaa atacagctga accttctcca tccactccag tttgaagtat atgagaaact
    16741 gcctttacag tttttggagc accaaataaa ttcaccatag gatgtataca atacactcct
    16801 atatccatta aagctccatt tgacaactct tttttaaagg cattttctat aattccattt
    16861 ttatatttat catatcttga agaatactga caatagcttc caaaaaatct tcttattttc
    16921 cctattttat acaagttttc tttaactgct ttaaagtttg gaacacatgt aattctcata
    16981 gcttccataa gcaatacatt attatctttg gctgttttta tcattgattt gacttctttt
    17041 aaattagatg ctaacgactt ttcacaaagc acatgttttt tgtgttttaa acaagtaatg
    17101 gtttgctttg aatgtaatgc atttggagaa gctatgtata cagcatctat atccttcgat
    17161 ttagccattt gttctaaatc atcaaaaaaa atacttgctc catatagagt tccaaattct
    17221 ctagcttttt ctaaatttct agaatataca gcaaccaatt caaagtcttt aaccctagat
    17281 gcagctctta gaaatatatt tgctatattg cttgtcccaa caataccaaa tcttatttta
    17341 cccatcataa atcactcccc attttctaca ctttatttta attttaataa aaaactttac
    17401 aacttataga tatattataa aataatttta tcaaataaaa aagaaagcta attttaaatg
    17461 atttcaccat tttaacatag ctttctttta actacaatct aaattcaact tatattttta
    17521 aagttatctt tctactatat ctttatttcc cctcaaaaat tttatagtaa gaatttcatc
    17581 cacaaacaat gttactaaaa gtatagttac aacaattggc caataaggta ttataagact
    17641 atataaacta tcaactatat acacttgaaa atatctaact ccaataaatg tcaatatagt
    17701 ccaatataca gaaaacttta gacatactat tcctctacta ttgtacttat aatctgaata
    17761 atcccaatag accttattaa aatatacttt cataagtatt cctgtaatat attctactac
    17821 tgtcggcact actagacata ataaaagcaa tgcaaatata ttattggggt caataaatct
    17881 tgagacctct attataatcg acattgcaat tgcatacatt ggtttaaatg gaccatttaa
    17941 aaatccatct tcttgaaaat gcccttgttt ataataacta tataagtttt caattatcca
    18001 acctataaaa ccatacaaga aaaaattaaa taaaaaatat aatagagaac ctacattatc
    18061 catgaatatt cctcctagct gtttatactt attttttctc atattcatgg atttattcta
    18121 aatcaaagaa attatattta atttatttaa ttaatacttt atattataga ttgtgacatt
    18181 ctttagagtt taaacttttt aggtattctc taacttccag taaattttca agtatcttat
    18241 gtgattgtga tttaatttgc tcatctggct cttttaaatc tggtacattt atacatctta
    18301 taccaccatt gtatgcagct tcaactccca taggcgaatc ctctatgaca atacaatttt
    18361 ggggattaac atttatcttt ttagctgctt ttaagaatat ctcagggttt ggtttagagt
    18421 ttacaacatc atctccacaa actatagcat caaagtaatc ttttaaattt gccttagcta
    18481 atctctttac tgctctttct ctctttgttg atgttgcaac agccattttg tatccatttt
    18541 cctttaaaaa agagattagt tcattgactc ctaatttgat aggagctccc ttcctctcca
    18601 taaattcaat catattttta gttttttcgt catataaatc tattattggt acagaactat
    18661 cgtatatatc tgtaagacct tctattattc ctttacgatt tcttcccata actgatgtat
    18721 aaatttcctt agtcatagta tatccatact tttcaaacgt ctccatccaa aattcaagag
    18781 aaattctctc tgagtcaaac aaaactccat ccatgtcaaa aataattcct tctacttttt
    18841 gcatacaatt cctccaatat tttttgatat aattatatta cactttttat gattaatttt
    18901 acaaccctca atttatccaa ttctaatatt gtaaaattgt aattttcaaa agttacactt
    18961 tggtttactt taatatttga accaagcata taggatatcc atccacctat actgtcaaat
    19021 ccatcatgct ctatatcaag tccaaaatac ttattaatat agttgataga ttcagttcca
    19081 tcaactaagt aagaacctgc tcctattttc ttaatatgag tctcttcttt gtcaaattca
    19141 tcttgaattt ctccaactat ctcttctagt atatcttcta ttgttaccac accagatgta
    19201 ccaccatatt catccacaac tatagcaagt tgaagttttt cctttcttat tctctcaaga
    19261 gctttatcta tagttaaatt ttcagatata tatataatat ctctcaatat ttcttctagt
    19321 tcaattttat tttcatttat cttttgattg tataaatctc ttatatgtac aaaacctaat
    19381 atatcatctt tattttttct gcatactgga tatcttgtaa caccttcctc ttttaaaata
    19441 gctaatatct tttcttcaga atcactttca tatatacata ccatatcagt tctaggaacc
    19501 attatctctc taattttctt ttcttcaaat tcaaaaatat tatcaactaa tctttgctct
    19561 gattcatcta taagtccact tttataactt tcttctacta atagtcttat ttcgtcacct
    19621 gtatggggtt catcaacctc atcagcttgt gaatatccaa atggtttaag aaataaatca
    19681 gtacttaagt tgaaaaaata tattattgga tatgttaact tataaaatcc tactaataaa
    19741 aaagatgtac tcaacattat tttttctgta ttataaagtg ctaaagcttt aggaaccaac
    19801 tctcctatta caacctccat cattgttata attaaaaaag aaatggaaat tgatatagta
    19861 tatataatac tttcactaag attaaataca cttactattg gtaaaattaa ctctttaact
    19921 gtagattctc ccatccaacc taatgcaagt gaacaaagtg ttataccaag ttgacatgca
    19981 gaaagatagg aatttaagtt gtcttttatt attttacatc tttttgcatt attattacct
    20041 tcagttatca tggtatcaat cctagaacta cgaagtttta ccatagcaaa ttcacaagct
    20101 acaaaaaaag catttatcaa aacaagcatt actataagtg ttacttttaa taactcccgc
    20161 ataaagtatt ggttcttata atgtaagaaa tctataagaa ctcaaagttc cccccttata
    20221 tttataaatt taatttataa tatatattct atcatattta ttaatatatc ctactttatt
    20281 acaaataatt tttataaact aaataacccc ttttaatatt tatgactggc tatttgataa
    20341 atattaaagg ggttatttag ttatcatttt ttatttctaa aaatcttttc tcaaatgatt
    20401 tagttatcta tttatccaat tttttacata atttaatgat taaaaatatt aattttttca
    20461 ctataaagta taaatatttt ttgtataaat attatctgat tattaaaata aatattaata
    20521 ctataaataa aaacgaagca tgatttttat ttaaataaat ctcaagcttc gtctttactt
    20581 acttagatat ttatttactt tctaaatcct attattttta aaaccttatc tttttaatac
    20641 ttcaccacat ttattctcag taatctctcc attttccaac gcaatttttc catttacaat
    20701 tacatattca acaccattgg ctagtaattg tggctttgaa aaatcaatat cttctgacct
    20761 agaataacta tagttatcca tgtcaataac tgctatatca gctatcaacc cttctttaag
    20821 aatacctcta ttttttattt taaattgcct agctggtaat gaagtaactc ttcttacaat
    20881 ctcttccaat ctaacaccag actttctgta catattgaag aaaattggaa aagctcctct
    20941 tctttgtagt tcaaaccatg acatatcccc acccacacta aacaaacaat ctgaaccaac
    21001 cataacaaat ggattatcaa ttattttatc atcatgtagt ttatctggga aatcagctct
    21061 atacattcct cctaaacaaa agataagttc ttcgtctcca tctaaaagta aatcgagtaa
    21121 taacttgtct tcatctattc ctttttgtgt agctatttca cgtattgatt tcttttccat
    21181 attaatatct ttagtgttta gaagtattat actcccccta tctccaagta taaatgcttc
    21241 ttttaatata agtgttcttc cctcttcatt tctaagagca tccattaccc catttatatc
    21301 taattcaaat agactagatg atattgccat aataaattga attactcttt ttttcttcat
    21361 acaatgccca gaacttcttg gtactgtgtc aaccattatt tcaacaccat tatacctagc
    21421 ctttaatatc tcatcaaatg cttctatttc aactggactt aagtgagata cttgaaccct
    21481 agcaccactt ttttgcccaa cctctatggc ttcttttact gcattatatc ttcctatgta
    21541 atctctaata tgagatgtat atataccatc atgctcttta atttgccttg caatatcaac
    21601 aagttcatca atatcagcat acttacttgg tatatatgcc aatccagtag atattccaaa
    21661 agcaccactt ttcattccat catttattat atctgtaatt tcattttttt ccttttctgt
    21721 aggaggtcta tccttagaac ctcccataac actccatctt atagtcccat gccccaacaa
    21781 gaatcccata ttgacagaaa taccactttt agatataagg tcacaatatt ctgtaaaatt
    21841 attccaacat ttatttttat ctataagata ctttttttct tcttcaagta atagaccatt
    21901 tttatacata tattcataca cattttcaga agaatatggt gttatagaat gtccacaatt
    21961 accatttata gttgttgtaa ctccttgttt tagaaacttt tcaaatttac catctagtat
    22021 tgctactatt tcttcatgaa catgagggtc aataaatcca ggtgttataa cttttccaca
    22081 acaatcaatc tctttatttg tattgctatt tatcttagaa tctatttttg ctattaagtt
    22141 gtctttaatt gctatatctc cataaaaacc aggattacct gtaccatcta ttataaatcc
    22201 attttttaag attaaatcat atatcaaaat attccctcct taaaaaccaa tttttaaata
    22261 atcttaatgt aatttttata tttgaaaatc aaattattaa atttcatatt tgaaaattca
    22321 ctattatttc attgtaatta gtcactaagc ttatctttat acatagctct tgcaattaat
    22381 atagtaccaa atacacatat aagaactact agagactgtg gtattatacc ttgctttgat
    22441 aatataatag tcaatacaga tattacaatt gctactagac ctaatttttt atcttgtaaa
    22501 aatacttgtc caaaaactga cccaaataga gctggtaaaa ttaagtttaa tttttctact
    22561 acttcagctg gaattttaga tagtacgctt cctcctaaaa ttacccctat agttagtata
    22621 cttatattca ctaaaataga tacagctatt cctattgtag atattatcga gccttcttct
    22681 gtacctgatt caacttctgc tgccttttga gctgctattg aacatggtag cctcatatta
    22741 gatatattac cagaaagaaa actcatataa gttcctggta tacctaagat tggtgaataa
    22801 gaaactggtt ctattatgta atttggagca ctcatactga caattgccat tgtacttgct
    22861 aataatgctg atattggagg cataatacca aatccaaagc tcaaaattat tccaggaact
    22921 aatgctgcta taatacccaa agatagtgtt aatcttccat accttatcat aaaaggtaaa
    22981 tattcttcat tgtatataga ttttacttta ctcataaaac taactccccc cttatattac
    23041 tgaagctatt atcattccgc taaacattga tattgtaagc ccccactctc ttaaccattt
    23101 tatatttttc ttcttttcaa gcatacacaa tattaccatt atagccattc cacttataca
    23161 tgcaattgtt ccattatcaa aacgcaacac cctatcagca tttagatatg caaatgaacc
    23221 caacattgct cctagagata ttattggtat aaatgatttc ttgccactag atataacatt
    23281 ggttatctta tctagtttat gtccaaatag taatgtgaat attatccatc ctaaagaacc
    23341 taatatcata gtccaaactg catttgtaaa tgcaagtttt gtcatttcag gagaacctaa
    23401 agttatacct aatgcatctg ttccaaatcc ggctgacatt gattcaaaaa ttacagaacc
    23461 tataaatgat aatctcatcc atgctatagg cccacccata gttactataa gcgataccat
    23521 tccagcaaat atagtcattg aaggcccaat agctgaaata gcactactct taacagctga
    23581 ctttatttgt ttatctgtta ttcccattgt tttaccaact ttgcaagatt ttatagcaaa
    23641 tataagggat tgtaccacta ctattgatac tgctactgta ctcgctatcc acaaaagtgg
    23701 atgattagct aattttaaat aatccattta taaaacctcc ctataaatcc tcaaatttta
    23761 ttaatatttc taatatatct atttccaaac ctgttttagt actctaagta tatttccacc
    23821 tagtaccttc tctatttcct catcactata tccatgcttt actaaatatc taacaatgtt
    23881 ttttgaagtt tctgttgggt tctcaagacc ttttacatat tctactttgg gatgttcttt
    23941 tgttcctgtt atttttttta tatcaaattt agcagctagt actgagtgta attttacatg
    24001 gtcgccatat actgtatctg gtccaaaagc tacatggtct attcctacta aatttttaat
    24061 atactcaaaa tgttccataa atgactctat tgaatgttct ttattcttct ctgttattgt
    24121 agtatgaggt gctgcttcta cacctataac ccctcctttt ttagcacatg ctattaatac
    24181 ttcatctgga gttagtctct ttgtgttcca taaacttctt gccccaacat ggcttaatat
    24241 tattgggtcc ttgctatatt ctattacatc aagtgtagtt tgaactccaa cgtgagagca
    24301 atctattgcc attccaattt tattcattct ttcaacagct tttttcccaa aatttgtaag
    24361 acctccatct ttctcttctt ttagtcctga acctaatgca ttggattcgc tgtatgttat
    24421 acccattaac cttataccaa atccatacag tatatctatt ctatcaagtt cattttctat
    24481 tggtgctgca ccttctaatg ttggtatcaa tgctacttta ccttcacttt tagctctgta
    24541 tatatcttct actttttcac actttatgac aaaatcttga tgagctaaat cacataatct
    24601 catgcctaaa tctattaata tatcatccca tttccaccct gaatttgatg ttatcgtaca
    24661 tacaccatcc attaaattat caaatatagc atcataatat cctcttgata atgcttcata
    24721 agcacatctt tgtttacctt ccctattata ttcaaatata tctctttcta ggtgttctgg
    24781 aaataatact ggatgttcat gtaatgatat aaatattttt tcatccacta tacttttaac
    24841 cttagcttct tgaatatcat ctaattcaat aaggtattct ttaactcttt tatctccttt
    24901 acacatctca atagatgaaa tatcttttcc tttttctaaa taactatagg cttcatatcc
    24961 tttgtaagct gacttcataa caacatcctc caagttctac aattttttat atttttccta
    25021 tgtagcaatt gatgtgccaa aatataaatt ttaattgtat aataaatctt gcaaaaaata
    25081 aaaacagtag taaggttgat atttactact gttttattaa aaataatctt cttagattat
    25141 ttattcaata tttttgaatt atcttaatct taaagataat tttatttatt tctatccatt
    25201 atagggattt ttttagggtg ttttgccttt ttagtcccct atatcactcc cttaattttt
    25261 acatatgata taaagttctt tccttcatca gttataaaac taccttgttt ttttactcca
    25321 gataaaagta aaccatggct ctttaactta tctattcttt ttctaatttg gtctacactt
    25381 aaaactatat ctttttcttt taaaatctct tgcagtttat ttcttcctaa taatatattt
    25441 ttattattcc aagtttctat actggtcaat atacatatac tttcctcaaa gaaattagat
    25501 tgtttgaaat ctaagataat tgaatcgaaa ttttcattaa ctaaagtatt gtttttttca
    25561 aatctaaatt gttctggcaa atgctcataa tctactctat cttcctcaac tatagtatca
    25621 atataataaa ttaaattttt aagctctctt acatttcctg gccattcata caatgttaaa
    25681 gcttgcattg attcctctgt aaaacatttg ttgctattta tttcatctaa aaaatactta
    25741 cttataagag gtatatcttc cttacgttct ctaagtctag gtatctctat atgtaataca
    25801 ttaatcctat aatacaaatc ttctctaaaa gaaccttctt taattttttt ctttaaatct
    25861 ttatttgttg cagctataat tctaacatct attggtataa ttttacttcc gcccattctc
    25921 ataacttctt tttcctgtaa tactcttagc aatctctgct gtacatctaa agatatatca
    25981 cctatctcat ctaaaaaaat tgttcctgta tgtgctcttt caaatatacc catttttcca
    26041 ccttttattg ctcctgtaaa acttccttca tcatagccaa aaagttcact ttcaataagt
    26101 gtatctgaca aagaagatag attaactgca acaaatggct tgttttttct tagagattca
    26161 ttatgtattg cctgggcaaa tatttctttt cctgttccat tttctccaag tatcaacaca
    26221 gaaaagtctg tagttgctat ttttcttgca atattaattt tttcttttat tattttactc
    26281 tcacctacaa cactctcaaa tgtatacttt gatacaaaac cttttgactg aagtttattt
    26341 tgaatcttgt cctcaagcac ttgaattgca cttatatttt taatgcttat aatactcttt
    26401 attcgttcat tattttcata aacatttact ttattaatta tcagtttttt gttatttaaa
    26461 tttactactt catcattaac ttcatcttct tgaaaaaata tttttttgac aaccttatca
    26521 ctaaataagt ccataaagtt attagatata atttcatttt ggtctattcc aactagatta
    26581 gaaaatactt tgttgcaata tataaatttt cctaatttat caacagaagc aataccttca
    26641 tctataatct ccaaaaaact cttcatggtt ttattcatag ttgtataata cctatatcca
    26701 cttactgtat cttcattgta tttttcctta ataatatgta gtttatctat agatatattt
    26761 agttttgtaa atatttctat aactgtattt atatcaatta ccttatcacc tatgtcaatt
    26821 atttgcttta tgttttgtgg tatgctgttt ctgcttcccg taataattcc aatctcacat
    26881 ttagacttat cacaacctgg ataatatggt attagattaa tatgaattaa tcctagtttt
    26941 ctaattatct tggcagtttc atctgctgac tctttataag catctataac cataacatca
    27001 gaatcatttt ctatacttat tatctgattt atattttcaa tatttatagt acgatgtact
    27061 attacaatag gtatattttt atcaatatta ttatgtatat attcctttat atcattgtct
    27121 gaacatacta caagctcata gcttgacagt tcaatattta tcctatttac atataccaag
    27181 ttatctatat tacaaaattc tccaaatata ttttccaatt gttttgatat atctatattt
    27241 attttgtcat cagttgatac aaataaaata ttatgttttt tcatagtccc acctcctgtt
    27301 taacgagtat agaaagtaat aatttggtct attgtagcac tttttaaaaa agttttaaat
    27361 cttaaaaatc aaattttgta ccatttacta ttattttcca taataaaata atgtaagcta
    27421 tcttttttca catataattt aatttctaaa acggttagtt tgacattatt ggaatataaa
    27481 caaaaattta atataaattc gttaatatat taatacattt ttaagaatga caaggaataa
    27541 attataacaa attttaggaa tatataataa tagggtctca gaacttagtt acaagttcta
    27601 aaaccctatt tgtttagtgt aaaaaacatc ataaaattta gcttaaatct tttcacataa
    27661 tattactctt tttctaagtg ataataaaat ttatctattc ctctaaaaac tgtaaaaatt
    27721 cctgattaaa tagtttaagc tcatcataga atatagcatg accactatac tcaaaagtat
    27781 ataaaattga atcttctatt ttttcattca taaaaactgc aaattcatac ggacatattt
    27841 catctaattt cccatgaaat ataccagtag gcactcttac acattttaaa tcattaaata
    27901 attcttcatc tcttaaagat acagcagttc caatagtgcc aatacctgat gcactaaaac
    27961 caatatcatt aaaccatctt ctaaagcttt ctggtggatt tgatgcaaat actttttcgc
    28021 caaaatcggt taccatttct ggtctatcag tacatgcttg tgctatcaat ttatttacat
    28081 cttcacgtgt cataccatat ggaaattctg gaggtctttg tacaaatgat ggtgcagcag
    28141 ctgcagctaa taccaatttt gacactccat atccattaaa taaactcata tacctaagta
    28201 cgattgctcc tcccattgaa aaaccaacaa gggtaaagtc ttttaatcca atagcatgta
    28261 ccaccttata aatatcgtct gctaattggc tataagtata tcctcctgat gttgcatcag
    28321 atttaccaaa acctcttaaa tcaattgaaa ctgttctata tccgagtttt ggcaatatat
    28381 ttgtttgata ttcaaatatc ttatgtccta agggccaacc atgtataaaa agtacagttt
    28441 ttttagcact cggattcaaa tcatatactg ctatgtttat atcattcaca tatacataat
    28501 acatatcaac agcaactccc ttcaagaaaa tgtatgatgg ttattttata tatatgaata
    28561 aaatgatgaa taactaaatt ttcaaattta tttacaatac ttctctattg ccttttcaaa
    28621 taaacttaca tgaagttttt catctaggat tattctattt aatatagctt ttatataagg
    28681 gtcttcaata agacttatat ctttataata attattaata gccacatgct ccaaatgcaa
    28741 atctagtttt aatctgttac aaatagaatt tccataacat acaaaaccac cattccaata
    28801 tgcaccacaa gaacttgtag aacctctata tacaggattt ccacccaata atcttatagt
    28861 ctttgctaat atctccatat gtaacatttc tgttatagat atatttatcc acatttcact
    28921 taactctcta tgcgtttcat ctaaatcaaa attatggtat agatattgat ttacacttgt
    28981 aaattcactt gatactccgg catagtcatc catcaataat tctgcatagt acttatttgg
    29041 acctaaaact tttatctctg gatatggctc atcacttgaa tatccatttc gcttgtgcat
    29101 gtcataatgt gcatcactca taatatctcc tcctttaaat ttaatgtatg atattaattg
    29161 tatgcatcac aactttttca tataacttag ataataaaat ctacttaaat aataaaaagc
    29221 catattaatt aaaattaaca tgacctttta tccacaaaat tactttttaa ttattcatct
    29281 tttccagcta ctgatagctc tttaataaca actgaaggcg aacctatact actcattgga
    29341 aattctaaat ctgctcctat agcttcaata tcttttaaca ggtcaaaata gtttcctgct
    29401 actgtaattt gctctactgg aaatactttt tttccttctt tgatataaaa gcctttggca
    29461 gctaaagaaa agtctcctgt tactgaattt gcacctgaat gaagtcctgc aaagctagta
    29521 accatcaatc cttcatctat atcctttata atttcatcta aagatttatc tcctttctct
    29581 atataaaaat tagttggata tatgcttata ggtgatgaat atgaagattt tattccattt
    29641 cctgtagtct ttacacctgc cttattagct gtttttaagt tatgaagtaa agttataagc
    29701 tttccattta aaacaacttc ttttttaaat gtagctactc cttcatcatc aaaaggagta
    29761 gatgccatac cattttctaa aagtgggtca tcaactattg taactatagg agatgctatc
    29821 atgtctcctt ctctattttt caataaagat aatccttttt gagctgaatc tgcattaaat
    29881 atcccagaaa atgtgctaag caaagatacc atagcttcat taagcaatat agttttatat
    29941 ttacctgatg gaatactctt accacctact ttagataaag cattatcaac accaagtttt
    30001 gctatctcac aaggttttat ttcttcaata gaatttgcta tattatatcc tattccatca
    30061 tatttttgtc cattgtcttc tatgatagga actacaaacc ctataagcat attagtttta
    30121 ttgcttaaat taagaccttt agtattagat ataccattac ttgatacaga ataagatata
    30181 gtacatttag ataaatttac caccttatct gaataagctt tagtctctct ttccatttct
    30241 aaagctaaat caatcaactt atctgcctct aaattttcta gctctttaga ataagtcttt
    30301 acatcattat agtgcttatc tccttcatat ataaattgta catcttcatt ttcaatagtg
    30361 tttactccat cttttacatt ttttatcaac atatcaacag ctttatcatc taaaatttct
    30421 gtataagaat aacctatctt tccatttacc ttacctctaa aagataaacc aaaagattta
    30481 tcaagattgt atttctcaac ttctccttca tatatattta tacttaaatt ttctcctgta
    30541 taataatata tttcacactc ttcaaaacct tcacttagag cctttttaaa taatatatct
    30601 ttaaaatttt taagttccac aattatccct cctatcttcc acctacagta atttctttta
    30661 cccttatcat aggttggcct acattagttg gtatacttcc tgatgatgag ccacacatcc
    30721 cctgagcttg ttttacgtta ttcccaacca tatctatatt cataagtata tcgctacctt
    30781 ttccaattaa actagcacct ctaactggct cttgtatttc tccatttttt attaagtaac
    30841 cttctgaaac tgcaaaatta aactctcctg ttacaggatt tacagaacca ccacccattt
    30901 ttttagcata aagtccatct ggtattgact ttataatatc ttctggtcta tcctctccag
    30961 cagcaatata agtgttagtc atccttgaag ttggtgcaaa tttgtagttc tgtctacgag
    31021 aacttcctgt aggattcatt cccattcgtc taccattaag tttatctatc atataagact
    31081 tcaaaattcc attttcaatc agtatattct tttgagttgg atttccttca tcatctatat
    31141 tcatagaacc ccagtagttt ggtattgtac catcatctat agctgtaatc tttgtggaag
    31201 caatctgttg acctaattta tctgcaaata cagagttccc ctttgcaact gatgtagctt
    31261 ccaaagaatg tccacaagct tcatgaaata ttactccacc aaatccatta tctattgcta
    31321 ccatcatatt acctgcagga caatttttag catgcaacat agtatgcgct actctagctg
    31381 attcttttgc ataatattct gggtctattt cttcaaatat ttcaaaccct ttacaacctc
    31441 caggaccttc aaatccagtt tgattttcat tgcccttaga tgcaattgaa cttatcccca
    31501 gtctggttct tattcttcta tcctcaacat atataccttc agtgtttgca attaatattt
    31561 tttggtcttt atccaaatag ctaacactta cttgagatat atcattacta aattcttttg
    31621 cactcttata tgcaaccttc ataacactta ctttatctct atttcctatt gaatttggat
    31681 aaatttttat attatgtatg ttatttattt taatactgtc atttaatatt acactttttc
    31741 catcttccaa ttttcctaaa gcaacagcag ctttgtatgc tacatccaac agactattta
    31801 atgaattgtc acttgtataa gcatatatac ttttcaatcc tttaaatatt cttattccta
    31861 tgccataatt tcttccacct attgcatttt ctactttatt atctattaaa ctaatagaat
    31921 tatttatagt gtcttcttca aaaatctcag caaaatctcc tccagtaatt agacactttt
    31981 ctaatacctt agaagctata ttttttgaaa gcataaattt acctcctact ttaaattttt
    32041 aattaacttt tattcaatct ttttacttgt taacaaacat tatatactat attacttaaa
    32101 ataaggtaat tatgaatata ttatacatat gttttatact aaaatgttat cacattttat
    32161 ctatacatac ccatataact agataattaa aatgtataat attatttgct atagaaaata
    32221 atacactcat acataataaa ttttaggagt gaagtatatg aatcaagaat atgtaaaagg
    32281 aaatcctatt tttactaata ttaataaagt tccaagacaa tttccatacc ttactgatga
    32341 tattgatact gatgttataa ttgttggtgg tggcgtaact ggatgtatct gtgcttacta
    32401 tctagctaaa aacaatataa agtctgtcat tcttgaaaaa ggaaggattg cacatggaag
    32461 cactagcgtg acaacttctc tattacagta tgaattagat gacaatttga tagatttaac
    32521 agaggttatg actctaaaag atgctctaaa agcatataac ctttgtactt cagcacttga
    32581 agaattagat acatttatag agctatatgg aaataagtgt gattatgcca aaagagatac
    32641 tctactttat actgccaaca aacttgaagt taaggctatc aaagaagaat ataacttaag
    32701 aaaagaaaat ggatttgatg ttgaatatat tgatgaatcc acaaaccctt ttagctttga
    32761 tttaaaatca ggattgatag caaagaatgg aggacgagaa ttagacccat ataaatatag
    32821 tcaccatttg attgatgtaa gtttaaaaaa tggtcttcaa gtatatgaaa atacagaagt
    32881 aaaaaaagta gatttatcaa atgataaggt tactgctgaa gtatcgtatg gatacaaagt
    32941 tcatggtaaa aaattaattg tagctacagg gtacaataca agtttattta caaaaagaaa
    33001 ttttgcaact aagtcaaata cttttaatat agcaacgaaa ccacttaaaa acatagcttc
    33061 ttggaaaaac aacattttaa ttagagataa ctgtgaccca tataattatc taagaactac
    33121 taaagacaat cgattaatta taggtggtga agatgtaaat tttgatgata tagaaaatga
    33181 aactttagcc aatgaaaagt acgatatttt agaacaaaga ttgaaaagta tgtttaagga
    33241 tataaaagac attgaagttg aatataaata ttgtggttgt tttgcttcaa ctttagataa
    33301 tttaggtttt attggtccag ataataaaaa taacaatcta tggtattgct taggttatgg
    33361 agcaaatggt atactctttg caatattagg aggtataatg ttaagtgaat tatatcttgg
    33421 caaacaaaat aaaaatatga aattatttaa agtagataga tttgataaat agatattata
    33481 tattaaaaga ctgtaaaaag atgaaacttt ataattctaa aagttaactt tgatacagtc
    33541 ttttataatg ttccttttaa aaatacttta tttataatta aatataaagt aaaatgttaa
    33601 aatataactg ataaaattta gtcataagat aatatttaag tttatttgat gaaatatgga
    33661 ggaattatta tgaaggaaat actcgttatt gctcccacta aagaaaccta tgaaaaatca
    33721 attcacatag ttaaaaaaaa caaatataca aatattgatg ttgtatttgg caatctaaaa
    33781 gaaggtatac ctcttgctga aaaaagtatt aatcatggta ctagaattat cataagccga
    33841 ggtggaactt acaatatgtt aaaagctact tacaatatac ctatagttga aattaaggta
    33901 gatgcttatg atattataaa aagctacaag gaggtaaaaa attccaacga accatttgga
    33961 ataattggat ttaataatgt catctacggt ttcgatatta tagaagaaat attgaataag
    34021 aaaataacta tgattgaaat tgaaaaagaa gaagaaattt atgatgctat agaaaagtac
    34081 agaaagaaag gtataaacac atatatagga gataccactg ttgcacatat tgttaagaga
    34141 cttaattgta agggaatatt aatcgaatct agagaagaaa acatacttag agccattcaa
    34201 caagcagaac aaatactcga agcaaccaag gatgaacaaa aaagaagatt acagatagaa
    34261 gctatgacag attttgtaca tgatggaata ataacagttg ataaaaattt taaaataaca
    34321 ttatttaata agagtgctga aaaaatattt ggtataaaaa aagaaaatgc actttatcat
    34381 aatgttgtag atgttatccc aaacacagtt cttccacaag tagttgagac tatggaacct
    34441 caaattggtg aaatacaaaa tatagggagt acaaaaatta ttacaaatag agttccaata
    34501 attgttgatg gtcagattca aggagctgtt tcaacatttc aagatagcaa ggagataagt
    34561 tcttttgaac atacaataag aaggagctta ttaagcaaag gtctatctgc taaatacact
    34621 tttgatgata taattcatgc tagtgaaaag acaaaagatt gtatagaact tgcaaaaaaa
    34681 tatgcttgtt atgatactcc tatgattatt actggagaat ctggagttgg taaagaacta
    34741 ttttgtcaaa gtatacataa ctatagtaag cgtaaaaatg caccttttgt tgctgtaaat
    34801 tgtgctgcca tacctccatc tctaatagag agtgagtttt ttggttatga ggaaggttca
    34861 tttacaggtg ctagaaaaaa aggtaaagca ggtgtattcg aactagcaca tgaaggcaca
    34921 atcttcctag atgaaataag tgaaatccct cttgaacttc aaggtcgatt attgagggta
    34981 ttgcaggaaa aacaagttat gagaattggt ggtgataaag ttattcctat agatgtaaaa
    35041 attatctgtg cttcaaataa agacttaaaa aatatgatgc gcaatggttt atttagacaa
    35101 gacctttatt ttagaataaa tatactaaca ttatatcttc cttcattaaa agaaagaaaa
    35161 gaagatatta taaaattatc agaatttttt atacataaat attcaagcaa atacaataaa
    35221 acgcctttaa ttatcactcc aaatattaaa aaggcacttc ttgaacgaga atatgaaggt
    35281 aatataaggg aattggaagg tatgatggaa agggctgtta ttgtggaatc atttgactcg
    35341 attttattag aaaagcttag caatgaatat acaaataata tagttgataa agataatagt
    35401 gattttgaat gcagtaattt agatttaaaa actttagaaa aaaaatatat ctataaaatt
    35461 tataacgaaa ataatagaaa tactacaaaa acctgtgaaa ttctaaagat aactcgctcc
    35521 actctatggc gtaagttaaa agaaatagaa gatgatgttt aaaaataata catatctata
    35581 tatatcaaaa cgttttatgt ttaattttaa tacataagac gtttatttgt tttcaataaa
    35641 aaatacaatt atatgataaa aagaatcttt gtaagtgaaa ttctcgtcaa tcatactttt
    35701 aattataagt aatctacagc atttcaatcg attattaaaa ataatcataa taactaaaga
    35761 tacaaactct atatattggc atagtaattg cttttataat acctatataa aaatgaaagg
    35821 gagtgatact agatgcacat cttaaaatca gtgaaaaaga taccaggagg acttatggtc
    35881 tgtcccttgt tatttggagc actatttaat acagtatgtc cacaagctct tgaaattgga
    35941 ggattcacaa catccttatt taaaactggt gcaatgagta tacttgctct attttgtcta
    36001 tgcaatggag ctcaaatcaa tatcagacaa gctggtaaac cattgactaa aggtatagta
    36061 cttacagcaa caaaatttat aattggtgca gtattaggta ttattgttag taaatttatt
    36121 ggacctaaag gtataattgg tattacacct cttgccatag ttgctaccat gactaattca
    36181 aatggaggtc tatttgctgc tcttgcaggt gaatatggag actccacaga tgttggagca
    36241 atatctatct tatctctaaa tgatggtcct ttttttacaa tgcttgcttt tggagtaact
    36301 ggacttgcaa atgtacctat agtagcacta tttgcagcac ttataccaat actacttgga
    36361 tttatacttg gtaaccttga tgaagatata cgagaatttc ttgcacctgg tacaacatta
    36421 ttaattcctt tcttcgcttt ccctttagga gcagcattaa actttaatca aattataact
    36481 gctggactac ctggagttgt acttggttta ggagttacta taataacagg tatgggtggg
    36541 tactttgtaa tgaaacttat acgtgcagaa catccagcag taggtgcagc tacaggtaca
    36601 acagccggaa atgcagctgg tactcctgaa gctttagcag ctatagaccc tactctagct
    36661 acaatagcag ctgtttcaac agtacaggtt gctgctgcta ttattgttac agcaatatgt
    36721 tgtccaatgt tagtttccta tctagataaa agagaaaaaa agaaaaaatc tatttttaaa
    36781 acaaataaaa atattgaagt aaattaatac ttaaggaggt aaaattaatg aatgcaatta
    36841 ttcatgcaaa tgaaaaagat aatctagtta catgtgtcag acctttagtt aaaggtgaaa
    36901 aagtttcaat tgatggaaag tcttacatag taaatgataa tatacctgtc tttcataaga
    36961 tggctacaga agatgtaaaa aaaggtgatg ttgtatacaa atacggtgaa atcataggaa
    37021 ttgcaacagt tgacataaaa actggtgact atgttcatgt acataacatt gaaagtacac
    37081 gtggtcgtgg agataaaaaa taggaggaag ttaaaatgaa attaatgggt tatttaagag
    37141 aaaatggtca gtttggtatt cgtaatcatg ttttaattat tccaacatct gtatgttcaa
    37201 gtgagacagc tacaagaata gcatcacttg tgcctggagc aatagcaatc cctcatcaac
    37261 atggatgttg tcaaattggt tctgatatag agcttacagc aaagacatta ataggatttg
    37321 gaaaaaatcc taatgttgcc gcagttttag tagttggtct tggatgtgat ggtattcaag
    37381 caaaagaatt agcatctgaa atagctacaa caggaaaaaa ggtagattac gttgttatac
    37441 aagaatgtgg tggaacatta aaaacagttt ctaagggagc tgaaatagta agtaaaatgg
    37501 ctcgtgaagt gtcaaaagaa gtaagagttg aatttggtat gagtgaaatt acattagcac
    37561 ttgaatgtgg tggttctgac ccaacaagtg gaatagcttc aaacccttca ataggcgttg
    37621 catcaaacct tcttgtagat gaaggtggaa gtagcatact aagtgaaact acagaagtaa
    37681 ttggtgcaga acatttgttg gctacccgtt ttgaagatga agaaatgaaa gataagtttc
    37741 ttaaatttgt aagtgatgta gaaaaaagag caattgctat gggagaagat ttacgtagtg
    37801 gtcaacctac tccaggcaat aaagctggtg gactttcaac aattgaagaa aagtcacttg
    37861 ggtgtatgta taaggcaggc aataagccat tcaaaggtgc tcttgaatat gctgatattg
    37921 tacctcctga taaaaaagga ttatatttta tggatactcc aggtcaagat atagattcta
    37981 tcactggtat ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc
    38041 caacaggaag ccctatttca ccagttatta aaatcactgg gaatagtgat acatacaaca
    38101 aaatgccaga caatatcgat ataaatgcag gtagaattat aacagatggt gctaaaatag
    38161 cagatatagg tcaagaaata tttaatgaaa taatagaagt ctgtaatggt aaacatacca
    38221 aagctgaaag tcttggtcat agagaatttg gcatttacag aatttcatct acattttaga
    38281 ttgtaacaaa atatctttta taaatataat acttatgtag atacataagc taaaagttct
    38341 ttctaatact ataaaaattt tctatactga aaaggaactg tatcaaaatt attttgaaaa
    38401 cagttccttt tttaacctct tttactcaaa atattataaa ataagattta aacaataaac
    38461 gtatcttgat taatctctaa gaagacataa ataaagctaa atgtaaagtg gaataaataa
    38521 aatttaatat ctttgtatga aaagatattt ggtataatat gtctgagaga attcttattt
    38581 gtaaacaaat aaaggatgga ggatgaaaca aaagaatgaa ctttgaaaac acaaaaaata
    38641 aaaaaataat agctacttta ctattgacac tccttttaat cgtgtcatta acatgtgtca
    38701 cttttattat gaatctaaat aaagtaacac ataatgaaac agaacgatat cttgtagaag
    38761 tttcacaaag tatttccact acaattaatg tgaaatgtaa aagtatattt aggacactat
    38821 attctacggg agatacttat tttgagatta atgatagtga aaaagaagtc aatagatact
    38881 tatcaaaaaa attaaaatta tttgaatttg attggttagg tattatagat tctaatggaa
    38941 attttgtaag tgctcttaaa gatacaggta atgttaaagg acaagacttt tatagaaaag
    39001 cccttcaagg caaagaaaca attactcctg ttaaatcaca atctggtaca tatggaattt
    39061 tatattctgt accactatac aatggcgata aaattgttgg agctgttgct gcttggaata
    39121 ctattgatac tatgagaaat ttattaagtg tagaaagctt tcatggtgaa ggattttccc
    39181 aaattatcga ccaagatgga aattttattg taagttcagc aaacaaaaat gctccaaaaa
    39241 atgtggtaaa tttttttgac atgattaaac accaaggaaa acaggttgat aaaaataaac
    39301 ttgaagaaat gaaaataaac ttaaaaaaga acaaaactgg ttctatgtat tatacattaa
    39361 atgatggtat ttctaaagca atgcactatc ttccattaca agaagaaaac ttatacctcc
    39421 tttcaattgt tccaacaaaa acagcaagtg cacagtttga tttacttcta aaaaaagcaa
    39481 tcttcatcaa tattttaatt gttatactat ttttattgct tattatattg attatgttta
    39541 caaatcaaaa gaacagggca caacttataa aaatcgccta tgtagactct gtaactaaag
    39601 gttacagtaa agcacgtttt gatattgaag ctaaaaaaat tatacagtca tcttcatcag
    39661 aaagctattc attggtatct ataaatattc agaaattcaa acttattaat gattcttttg
    39721 gtagtgaagc aggagataaa acattaaagt acatacatga tgtcattagt aattatctag
    39781 aaccaggaga attgctttgt cgagtttcta atgaccagtt taatgtactt attaaaacta
    39841 ttcctcaaaa agaaattctt aagtacatag aaaatatcac caatgatatt aatagtttca
    39901 ataaaaatga agaatgtaaa tattttatag ctctatctat aggtgtatat aaaattgatg
    39961 acccaaatct acctttagtt tcaattcaag accgtgcaaa tgttgcacga aaaaataata
    40021 aaggaaacat tggaaatcat ccactttata catgtgtttt ttatagtgat ttagagcgtt
    40081 taaaaatgat taaagaaaaa gaaatggaaa atagaatgga ggaagcatta aataataacg
    40141 aatttatagt atatcttcag cctaaaataa gcctcaaaga taatactatt gtaggtgcag
    40201 aagcattagt aagatggcaa gacccaaata aaggtcttat accaccagac gagtttattc
    40261 ctttctttga aaaaaatggt tttattacaa aactcgacca ctatgttttt gaaaaagtat
    40321 gcagtatgat aagaaaatgg atagataatg gagaaactcc aatacctgtt tcagttaatt
    40381 tttcacgtgc taatttaaat aatatagatt ttttagaaaa atacagaatg cttcgtaata
    40441 aatacaacat tcctgctaag ctcttagaaa ttgaattaac tgaaacactt gtatttgaaa
    40501 atctccaaaa attaattaat ataatagacc aaatccatga agaaggtttt caatgttcac
    40561 tagatgactt tggaagtgga tactcttcac tcaatatgtt aaaagaaata aaagttgaca
    40621 cattaaaact tgaccgtgct ttttttagct cacctaatgc agataatatt gctgaaaatc
    40681 atgtaataga atctatagtt aatttggcaa aaaaattaaa tatgaatagt atatcagaag
    40741 gtgttgaaac aatcttgcaa atggattatc ttaaaaaaat taattgtgat atgatacaag
    40801 gatatgtttt ttcaaaacct ctgcctcaag aaatatttga gaaaatgact tttggaaaaa
    40861 aactagaaga aaccagtatg gatagttccg tttaaataaa ataaaaaact tgtctctata
    40921 aaataatttt aatcatttta taaagacaag tctttttatt ttccaattta attttatttt
    40981 ctaaatttct tacactcggc ttgttttaaa ttcccttttt aagaataata atacgattat
    41041 catagcaata aaatctgcaa ctggctgtgc caaccaaaca ccatctaaat taaattgaat
    41101 tggaagtaca atgattaatg gtataaataa aataaattgt cttaaaagag ttaaaaacat
    41161 tgaatgttta actttactta tacactgaaa atacacaggt cctacaatag aaatccccat
    41221 tataggcaat gtaattagat ttaggcttat acctcttact gctatatcca tcaaatctga
    41281 atctttatta aatataccta caaatacttc tgggaaaact tgtactacta aaaatccaaa
    41341 tgacaatatg acaactgaca ctatgataga taatattaaa gttttctttg cacgctcata
    41401 ttgttttgca ccataattgt atgatattat agtttgcata ccttgattca atccaaacac
    41461 aggcatcata aacatcaata aaatagatgt aagtgctgtc atagctccaa tagctaaatc
    41521 tccaccataa acctttagag aattatttgt aaacatgtga gttatactta tagctacttc
    41581 catagcaaaa ggtgtcattc ctattgcaaa taccttcttt actatatctt tattcaattt
    41641 taagttacat ttttttagtt ttaaattgga cttccctctt ataaaataat gtattaccca
    41701 aaagaatact actatttgac agagtactgt tgctatagct gctcctttta tacctaaatt
    41761 aaatacaaat ataaatatag ggtccaatat caggtttaat acacatccta caatcattgt
    41821 ccttgctgct aattttggat taccttctgc cctaattgca ttattaagag aaaatgcaac
    41881 taaattaaat atacttccaa ccaatatgac actcatatag tcttttgcat aaaatattga
    41941 atctttactt gcacccaaaa taaaaagtat atcttctaag aaaactaatc ctaatatagt
    42001 tataattaaa gagataatta tagatagtgt caaagcatta cctaatatct tctctgcttc
    42061 ttctctattt ctttccccta actttataga caatcttgtg ctagcaccta ctgataccaa
    42121 cattccaaaa gctattatta gagtaaatac tggcatagtt acaccaagac ctgcaatagc
    42181 tatagagcca ataccttcca tagaaccaat aaaagctcta tctactacat tataaagtga
    42241 agttaccatc attgctatta ttgcaggtat cgaataccta aataataaaa tccatatttt
    42301 ttcatgtctt aatgcttctt gattctccat gttgtcacca tttacaccat gtcataagtt
    42361 atacaaattg gtttatttgt tcttgggtct gttgctattt ctgcatcaat attaaataat
    42421 tcttttagat tatccttcgt cataacatca tatgcatcac cttcacaaac tattttacct
    42481 ttcttaatac ctatcatgtg gtcagcaaat cttgctgcat tattaagttc atgtattacc
    42541 atgacaatag ttcttccttg ttttttattt agttcatcta ataattttag tatctccaat
    42601 tgatgtgcca aatctaaata tgttgtaggc tcatccagta ataatatatc agtttcttgt
    42661 gccaaagcca tagctatcca tgccctttgt ctttgtcctc ctgataaatc ttccatattt
    42721 ctatccttaa attctgcaat tccagtaact tctaaactcc aatttacaat ctctctgtct
    42781 tctttactag aattaccaaa tccactttta tgaggaaatc ttccataaga aattaattct
    42841 gaaacggtaa gaccactagg agcttgaggg ctttgtggaa gtacagccat atcctttgct
    42901 atttctttag acttcttttt gtaaatgtcg tctccattta tatatatttt cccactatta
    42961 gggctaataa ttctacctat agttttaagt attgtcgatt ttccacaacc attagcccct
    43021 atgatggtag ttatttttcc ttttggtata tttaaattta aattttttat aatatctgtg
    43081 tttccatatg atacatttag attcttagta tttatacagt tcataacaac accctttcct
    43141 aatcatttac caacattaaa taaataaagt acggcactcc tattatagaa actactatac
    43201 ctactggtat ttctataggt gctagaagat ttcttgatat agtatctgct attaaaagta
    43261 ataatgttcc tatcaatgct gatactggta ttaatctttt atgctttggt cctattattt
    43321 ttctacctat atgtggagct attaaaccta aaaatgaaat actacctgct actgctgtcg
    43381 atacaccaga taaaattact gcatatatta taagtcttct tctttgtttt tctacatcta
    43441 caccaagact tatagatact tcatcaccca aatttaaaac atctatatat ttagatttat
    43501 aaatagttat acccataaaa atcactataa atggaagcac agctattaca tatttccaac
    43561 tggttcccca aatgcttcct gatgtccatg ccataactct attaaattct tgtgttgtaa
    43621 atttcaattg gaatatcgtc aaaattgaag taaaagctat gtttacacct ataccaataa
    43681 gtagtaatct tgacgaattt ataccacctt tccatgcaag agaatatata agaaatgctc
    43741 caaataaagc tcctataagt gcaacaatag gcattgtaaa tacagtcaag ttactcatac
    43801 cttcatatac attaccattc ataagatata tataaactac tacaaacaat gcagcacctg
    43861 aatttattcc caaaattcca gaatcagcta aatcattctt tgtaactcct tgaagtatta
    43921 cacctgctgt tgataatgca gaaccaacca aaagtgctat tactattctt ggtaatctta
    43981 atttaaatat tgcaatttca tgattacgct gaccctgccc taaaaaagtc tttataacat
    44041 ccattggttc tatagaaaat gagcccatat ttaaactcaa taaaaaagtg ataaaaataa
    44101 gcaccattaa aattgatata acaagtaatg ttttagaatt tatttttata atatttttca
    44161 tagtacctat ttctccccct ttctaacaag gtatataaat attggcactc ctataagtgc
    44221 tgttatagaa cctactggtg tctcatatgg aggatttatc atcctactta atatatctgt
    44281 atatacaagc aatacagctc ctaatactaa agagctcggt attatatact tataatctga
    44341 acctactatt cctttagcta tttgaggaac tataagacct ataaaaataa tattaccaga
    44401 cactgacaca gatgctccag tcattaatat tacaagtact atacatataa atctaacaaa
    44461 gttagttttt tgtcctaatc ctattgccac ttcttctcca agacttaata tagttattct
    44521 tggagccata ataatagcta aaataaatcc aataccacca gctacaaata gcaattttat
    44581 accttcccac ttcactccta caagccctcc tgatatccag aaacttaatt gttgagatag
    44641 gttaaaatac atagatattg ctgatgccaa agaaattagc aatgtaccaa gagcgactcc
    44701 tgcaagcgct aattttacat tattaactct tcctcttgat ttagaactta taaaatatat
    44761 aaataatcca cttatacttg ctccaataaa tgcaaacatc attacactaa aacttccatg
    44821 tataagttga ggaagttttt tttgaagtac cagtgatatc gaaattgcga atgtagctcc
    44881 ttgtgtaatt cctataacag atggttcagc tataggattt ctcgttattc cttgcattat
    44941 tgccccagaa actgctaaaa atcctccaac aagtgcagct gctattgccc ttggaagtct
    45001 aacatccttt actattctca tatttattcc atcttccatt tttatcaaac tatctataac
    45061 agttgataga ttcatatctt ttgccccaat agttatggat aatacaattc caaatacaag
    45121 tacaactata cttactaata tcaacaaata agttatattt tttttactca gcttagtcaa
    45181 ttatcctacc tccttttccc ttttaatcgt cagtataata tcatctatta agtattttaa
    45241 ttttaagatt tattaaaaat aggtgtgaaa aatgaatatc attatctcac acctactaag
    45301 ttaagaatta ctttgttaat tcatttttaa ctgattctaa taataactct tttccaattg
    45361 gattgtatga ttgactgaaa aatggagcag catcaagtat tgttacatta ccatctttta
    45421 ctgctcttat ttgagcccag atagcactat tttctaaatc ttttttatct gcttctgtag
    45481 caattacaac tatatggtct gcatctatat ctgttaaacc ttccatactt accattggta
    45541 gtgttattcc atcctgtttt ggcatattct taggtctagc taatttcata tcatcattga
    45601 ttagagtacc tattccacca tcactaaata ccataaattg acctgaactt gccaacactg
    45661 gtaaataagt tttttctcca ttcttttcta taacttcctt acctaacttt gtagcttttt
    45721 catcatactt ctgtagccaa cttttagctt cttcttcttt gtcaaataac tttgaaacat
    45781 cagttaactt gcttctccag tcatttgcat aatctttcat cataactaca ggagctattt
    45841 cttttaattg gtcatatatt ttttcttgtc tttgactcat tattattaaa tcaggattta
    45901 cttctaatat agcttccata tccatagtat ccatcattga gtgcccaact atctttacac
    45961 ctttaagctc ttctcttatg taacttggta atttatcagt ttcatatgag tctacatttg
    46021 ctgttgcaac aggcttataa cctgctaata atagttcttc actacttcct gatatatcaa
    46081 ctattttttt aggatttgat ggtatcttaa cctctccttt aacagattgt acaactcttg
    46141 tctctgattt atctttatcc tctgtattct tatctgaaca tgctgtaaca agtactaacg
    46201 tagttataat agatataaat attgcaagtg attttatttt tttcatgatt acttctccta
    46261 cttgtttatt atacttatat gatattgatt atcataagca catatttatt atagcatttt
    46321 tctattttta gtcaatatta ttttgtaaaa ggataaaaat tcctaattaa tcttaattat
    46381 atatagctta gataaggttt tcttatatta aacagtattt tttttaagaa ttggacacaa
    46441 atacaatgac ttttcagtta aacaatgata aaatgcaatt tatagataag gaatttagta
    46501 gggaggattc aaaacatggt acatttagta tattgtgata atgcaggaaa aaaaggagaa
    46561 aaggtcttag ataaaatctt ggctggaaca aaaacaatgg ttattagagg tgctgctggt
    46621 agaaaaatac cacatagtag agtatttgaa gatgagttac tttattttat ggaaaaagga
    46681 actgcaaaaa tatctgcaaa agccactgtt aaaaaggtag aaaactatgt taaattaaca
    46741 gaagatgaaa tttctaaaat tttaaatgaa aatcaatcaa aattaaattt atctgaaaag
    46801 caaaaagttc gttggcataa aaaatgttta tgcttagttg aatttgaaaa tgttgaagaa
    46861 ataacacctc ttgattttga ccatcaagga aatatggatg attggcttat tgttgaaaaa
    46921 attgaagatg tagttgttgg tactagtata ccttacaatt atgagaagtc taaattttaa
    46981 agtatcaata acataatact atattgctaa actctactta aataaaaaaa gagtgtataa
    47041 aaattatttt tatacactct tttttaataa taattttaaa ttacttactt tctaatcttt
    47101 catatacatc tataaattct tctgctaatt gttgaaaatt catagctgtc attaaatggt
    47161 cttgtgcatg aataagtata acacttattt cagttttatt accattagct tcattttgta
    47221 tcaattctgt ttggaattta tgagctttat gaattgattc tttcgacttt tcaatacatt
    47281 ctctagcttt atctatattc ccgctctttg cttcttttat tgcttcaaat gctagccctt
    47341 tcgcatcacc tgcaaatcca attattccaa aagatatctc tatcatcttc tcatccattt
    47401 attcgacctc ctaaatatct ctatagcttg atatgactaa tccattcttt tctaaaaatt
    47461 ctttgacttt tttagatgta agtatcttgt gctctttcgt tctatcaata gcataagatg
    47521 tagaatttaa aatataatca tctaaaaaag ctgggtgaga cataatatcc actacatcat
    47581 acttcattat ttcgtctata ttttttataa aatattcttc acttacattt tccttataaa
    47641 aactatctat taaaggaact actttagaat actctaaatt atattcaaat ccacctctta
    47701 tcggaagatt atacttggtt acaatcttct ctattactgg ctttaaactt actaaagtat
    47761 ggatatgatg gtgactatct aaatgagata tatctattcc aagccctttt accctatcta
    47821 tttgagcaca aagttctcta taaatctcat cacaatccat tttttcaata atttcgtctg
    47881 taatcctcct aataaaaaaa ccgtctttat caacaatagt ttttaagtta gataataatg
    47941 gtctaccaca acttaaagtc atatgaactc cacaatttaa agttttattt tctttaagta
    48001 agcctactcc atgttctacc ccaggcatat ttgccatcat agaagtagac ctaactatac
    48061 cattattatg agctgaaatt attccatagt tcacagcttc acaatatcca aaatcatctg
    48121 catttattat aattttagtc ataccataac ctactttcta tagacaacta tctattaaat
    48181 ctaggaagat aatctttatg agcttccaac atttcgtcta atatagtttt tgctaagtca
    48241 tcagatggaa tcaatgggtt tatacaaagt gccaaaagag cagattcata atcgccatat
    48301 acagctgctt tagctgccaa tacttcaaat gatttgattt gacttactaa tccatgcaca
    48361 gaatctggta aatatcctat acttattggt tttgggccat tttttgttat tacactactt
    48421 acttctaccg cttgttcatc tttaaaatct cttatagaac ccttatttaa tgtattaact
    48481 acttggatat cttttttgtc attgtatata gaacttatta aattacaagc agcatcacta
    48541 tagtaagccc ctcctctttt ttcaagttga ggtggtttta catctaattt ttcatcctta
    48601 tataattcaa ataattgttc ttccaattct ttaactactt gacctctagc ttttccctct
    48661 ttaaactctc tcaactcatc ctcaagcatt tctttagttt tatagtagta tctatggtat
    48721 ggacaaggta ttgcacccaa agattttaca aattcagcat taaaatcaat tgcctttata
    48781 ttttgcatac ttatatctgc tttgacaaat ttatcaattg cttcttttgt aacatcttct
    48841 ccatctaaag atacatttag accataaacc atatgattaa gacctgcaaa atccatactt
    48901 attctgtcac tttctacatt aaatagtttg gcaacatcat tttttaaatg aattggcacg
    48961 ttgcatagac ctatatatct tctaaaatta gtatatttga atacagcctc tgttattatt
    49021 cctgttggat ttgtaaaatt caccatccat gcatttggac ataattcttc cacatctttt
    49081 attatatcaa atataactgg tattgtgcga agggctttaa ataaacctcc agcaccatta
    49141 gtttcctgcc ccataactcc atgagataaa ggaatagttt catcttttat tctcgcatct
    49201 aaaagaccta ctctaagttg tgttgtcaca aagtctgcat ctcttagagc ttctcttcta
    49261 tctaaagtaa gatttatttt catatctact ccagcttttt ttaccatcct cttagctaga
    49321 tttccaacta tctctaattt ttcttttcca gcttctatat ctacaagcca taattccttt
    49381 acaggtaact cttcatatct atttataaaa ccctctacaa gctcaggagt ataacttgaa
    49441 cctcctccaa ttgtaactat ttttagtccc tttttcatct cattatacca tccttaccta
    49501 taatttaata tactctacaa aaccctatac atttatttaa aactctatac atttatttac
    49561 cgaaataaaa ctacctagtt gttaattttc aatttattag cttccatttt ttcagacgct
    49621 atgacaaatg gaatatatat taatattgat aatattaaat taaatagtgc taaagctccg
    49681 ccagtccagt cacctgtagc cattgctcca cctaatatag gaggtgttac ccaaggtatt
    49741 ttaggtaaaa ttactggtgc tactaatcca aaatctattg ctaagtatga tacagcacta
    49801 catattactg gtgcaagtac aaatggaatc ataaatatag gatttaaaac tattggaagt
    49861 ccaaatatca atggttctcc tatgttaaat attgcaggtg gaccacctaa tactatcatc
    49921 tctttacgtc ttcttccagc tattaataaa gctactatta atcccaatac cattccagaa
    49981 ccacctaaat atacaaatgc atcaaagaat gcacctgcaa gtacatggta tccttccgtt
    50041 gctcctgctt gagccaaggc agcattttct ccacctaatt tcataagtat agtttctgta
    50101 aaaggaagtg ctatatttgc accatgaagt ccaacagtcc aaagtatatg aattataaat
    50161 gcaataagca ttgtagaacc taaactatca gctacatttg atagtggtgc tcctaaataa
    50221 gtatttaaaa tatctgttaa gaaacttcca ttggaaagca tttttataaa tattcctatt
    50281 acagtaaata tcattatagt taacattcct ggtattaact ttgcaaatga tcttgaaact
    50341 gctggtggaa ctccatctgg catttttata gttaatttct ttaccttaga taatcttaca
    50401 aatatctcag ttgataataa tcctattaat atagaagaga ataaagctgc tgtacctaag
    50461 taggtttgtt gtatcattcc ccatccttca actactgtag ttcctccctc aggaactatt
    50521 ttaccaattt gtggagccat tataaaaaag atagataaag ctataagccc agattgtaac
    50581 ccatcactct cataagattt tgctagaaaa taagcaacac ctattactaa gaatactgcc
    50641 attgtaccta ttgctcccca ccaaaggtcg cctccaaatg tggtccagtt ttctccaaat
    50701 atacttgcca tcaaattttt gtatgcttcc aatggtaagt tatttatcaa tgttcccaat
    50761 gcacctacca tagttatagg tatcattact ataaatgcat ctcttactgc tactaagtgc
    50821 ctttgagctc ctattttagc agctacaggc actatatatt tatccataaa tgacatgaat
    50881 ttttccattt tacatcctcc agtacaactt tattttgaac tatttaactc tacagctcta
    50941 tctaacacag cttttccatt acatcttcca taatccatca tatttataac ttctactgga
    51001 atattatatg gttttgccat ttcagttgct tttcctaata catatctaac ttgagaccct
    51061 aaaagtaaga catcacaatt ttctatttca ttttttaagt tcacttctga tatagcccat
    51121 atttttgctt ctataccgtt ttcctttgct gcatcctcca tttttgtaac taataaacta
    51181 gttgacattc ctgctgaaca agctaacatt atttttatca ttttttcccc tccaaattag
    51241 attcattaaa tattttttaa gtcataattt ttttaaacta tacatttaaa atacgctttt
    51301 ctattaattt tttcttaatg tatatatctt atttttaaat gtatatacat ttaattcttg
    51361 atattattat ataccaaatg tatatacatt tcaagaaaac tttttcattt tatttaaatt
    51421 ttattcgaca acagcattca aagttaatgt atatccatag tgatatgtgt gtgagtactc
    51481 aaaaatctca ctattttgta acttacaaat ttggcttata tataaaagtg gctctggttc
    51541 ttctaatttt agattatcca tacactcttc attcgatatt acagcttgta cttctctctc
    51601 tgacatggct atcttgtatc cacacacttc ttcaacaaaa ttataaagag aattttcaca
    51661 atgagtctta tttaaatttg gaaataactt aactggcata taagtaatca tatatgaaac
    51721 tcttgcatca ttggctaatc ttactctttt tatttcccaa acattttctt ctaaacttat
    51781 attcaactta cttgataaat tttcatctgc ctgaactatt tcaagagata ccaatatatt
    51841 agatacttta tagcctttac tttccatctc tttagtaaat ccacttacag acgatatatt
    51901 atcatatata ttatttgcaa taactatact acctacacct ctttgtctag ttatatagcc
    51961 ttctttaact agattattaa gtgcagacct tacagtcatt ctagttacat taaattcttc
    52021 actcagctgt ttttcagaag ggattaaatc cccttctttc aaactgtctg aatttattaa
    52081 ctctctaaca tgattttcta taactttata aattggctct ttcatttaat tcactcctta
    52141 ttaaatcttt tatataataa gataatttta tatacaaaca aagaaaatgt ctatacattt
    52201 aagataattt aaattttatt cagaaatctt gtacaacttt ccgagtttta ataaagatat
    52261 aaatagactt atcattaata taccttttaa tatacttgat aaggttatac tccaccatac
    52321 accatttaga cccaacattt ctggtctaga tataaagtaa gccaatggta ctcttgcacc
    52381 agtcagtata gtaattataa tagaaggtat ataggttctc cctaatcctt tgaaagctcc
    52441 tgttgttatt atttctaaac acataaaaaa ttgagaatag cctaatattt taagataaac
    52501 tgctcctttt tctattgcct cactttcatc tataaaaata gaaaatatac tctctcctgc
    52561 aaatactaat actaaagtag ttattatacc aagtacagaa gatacagtta tagctatttt
    52621 gcaaccttta tttattctag aatacttctt ggcaccataa ttttgtccaa caaaactgcc
    52681 taatgcaact gatagccctt cagccgtcat ccaggaaatt gcttctattt gtgagcctac
    52741 tttttgtact gcaacagcca caggccccca tgatgctact attactccta atagcattga
    52801 gaatactgta aacataccac tttgtattgc tataggtaat cccaatttat acagaacttt
    52861 ataatattta gtctctattt ctttaaatag tcttaattta aaatattctt ctttactttt
    52921 aactattata tatataaaac atgtagtaac tactatctga gcaaatacag ttgcaatagc
    52981 agctcctgca acaccaagtt ttggagcaaa tccccatcca aaaattaata tagggtcaag
    53041 tataatatta gttattaatc ctactgtatt aatacgaaaa ggagttttac tatttccaag
    53101 accattgaaa attgcagtaa atactggatt aataaaataa aataccatac ctatagctac
    53161 tattatcaaa tactgtctag acatggttat aacttctaag tctcctaatc taaaaaaacc
    53221 tatcaattgt ttattaagta ctattaaaga aattgtatat attattgcta gtataatatt
    53281 tatttctatt gctgacttca tatatgattt agtagtgctt atattatttg caccaatact
    53341 ttgtgctact tttatctctc cacctacttt tgaaatcata ataaatgcca ttgcaagcca
    53401 tggaaaaaaa ccagctgttc caactgcagc tactgcttta cttccggctt taccaaccca
    53461 tatcatatct atcatgttat accccatctg tataaatgaa gtccccatta ttggaagtga
    53521 tagcttaagt aatttttctg taattcttcc ttctgttaaa ttaagtctgt ctttcatttt
    53581 ccacctcttt tatattaaat ttatttgctt atatagctag attaatcatt ttatttgatt
    53641 gagttcacta tccttcaatt tatattatca tatctcagga ttgcatctca atcatactta
    53701 tgtttaagat acagtatttt acctatctat gataaaatta atacttatga atataaatct
    53761 agccatttca tcaatacata ttctaaccct taacttaact tcaattaagt atacatatcg
    53821 taaccaatta gaatatttta tacggtatct tccattaata aacaaccaat atcaatgatt
    53881 tatatataat tttatttata cataaaatag tatactataa
//
LOCUS       DAEMHZ010000009        51195 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000009 DAEMHZ010000000
VERSION     DAEMHZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.009"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            295..1080
                     /locus_tag="KPK72_000489"
     CDS             295..1080
                     /locus_tag="KPK72_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2487734.1"
                     /translation="MIAKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIHRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSASTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDIAGKIYHNIGYKEINEWTILFK"
     gene            complement(1177..2001)
                     /gene="pdaA"
                     /locus_tag="KPK72_000490"
     CDS             complement(1177..2001)
                     /gene="pdaA"
                     /locus_tag="KPK72_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2487735.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            2222..5341
                     /locus_tag="KPK72_000491"
     CDS             2222..5341
                     /locus_tag="KPK72_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2487736.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLEKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSML
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNVFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            5359..5958
                     /locus_tag="KPK72_000492"
     CDS             5359..5958
                     /locus_tag="KPK72_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG2487737.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMKYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(6200..8329)
                     /locus_tag="KPK72_000493"
     CDS             complement(6200..8329)
                     /locus_tag="KPK72_000493"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG2487738.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            8595..8843
                     /locus_tag="KPK72_000494"
     CDS             8595..8843
                     /locus_tag="KPK72_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487739.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            9009..10400
                     /locus_tag="KPK72_000495"
     CDS             9009..10400
                     /locus_tag="KPK72_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG2487740.1"
                     /translation="MNFRDLFEKAIDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLVQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            10515..11984
                     /locus_tag="KPK72_000496"
     CDS             10515..11984
                     /locus_tag="KPK72_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2487741.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            12049..12642
                     /locus_tag="KPK72_000497"
     CDS             12049..12642
                     /locus_tag="KPK72_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG2487742.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            12824..14170
                     /locus_tag="KPK72_000498"
     CDS             12824..14170
                     /locus_tag="KPK72_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2487743.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            14352..14723
                     /locus_tag="KPK72_000499"
     CDS             14352..14723
                     /locus_tag="KPK72_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG2487744.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            14716..15405
                     /locus_tag="KPK72_000500"
     CDS             14716..15405
                     /locus_tag="KPK72_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG2487745.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            15925..16791
                     /locus_tag="KPK72_000501"
     CDS             15925..16791
                     /locus_tag="KPK72_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2487746.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            17033..17842
                     /locus_tag="KPK72_000502"
     CDS             17033..17842
                     /locus_tag="KPK72_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487747.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            17986..19050
                     /locus_tag="KPK72_000503"
     CDS             17986..19050
                     /locus_tag="KPK72_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2487748.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIYEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            19078..19797
                     /locus_tag="KPK72_000504"
     CDS             19078..19797
                     /locus_tag="KPK72_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2487749.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            19821..20405
                     /locus_tag="KPK72_000505"
     CDS             19821..20405
                     /locus_tag="KPK72_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG2487750.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            20407..21756
                     /gene="pabB"
                     /locus_tag="KPK72_000506"
     CDS             20407..21756
                     /gene="pabB"
                     /locus_tag="KPK72_000506"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG2487751.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            21746..22489
                     /locus_tag="KPK72_000507"
     CDS             21746..22489
                     /locus_tag="KPK72_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBG2487752.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYTKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            22523..23224
                     /locus_tag="KPK72_000508"
     CDS             22523..23224
                     /locus_tag="KPK72_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487753.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKEIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKISLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            23250..23822
                     /gene="folE"
                     /locus_tag="KPK72_000509"
     CDS             23250..23822
                     /gene="folE"
                     /locus_tag="KPK72_000509"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG2487754.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            23886..24677
                     /gene="folP"
                     /locus_tag="KPK72_000510"
     CDS             23886..24677
                     /gene="folP"
                     /locus_tag="KPK72_000510"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2487755.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            24688..25053
                     /gene="folB"
                     /locus_tag="KPK72_000511"
     CDS             24688..25053
                     /gene="folB"
                     /locus_tag="KPK72_000511"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG2487756.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            25046..25552
                     /gene="folK"
                     /locus_tag="KPK72_000512"
     CDS             25046..25552
                     /gene="folK"
                     /locus_tag="KPK72_000512"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG2487757.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            25533..26318
                     /gene="aroF"
                     /locus_tag="KPK72_000513"
     CDS             25533..26318
                     /gene="aroF"
                     /locus_tag="KPK72_000513"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2487758.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            26573..28363
                     /locus_tag="KPK72_000514"
     CDS             26573..28363
                     /locus_tag="KPK72_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG2487759.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            28381..29547
                     /gene="rpoD"
                     /locus_tag="KPK72_000515"
     CDS             28381..29547
                     /gene="rpoD"
                     /locus_tag="KPK72_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG2487760.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            29712..30401
                     /locus_tag="KPK72_000516"
     CDS             29712..30401
                     /locus_tag="KPK72_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2487761.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            30415..31512
                     /locus_tag="KPK72_000517"
     CDS             30415..31512
                     /locus_tag="KPK72_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2487762.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            31575..32147
                     /locus_tag="KPK72_000518"
     CDS             31575..32147
                     /locus_tag="KPK72_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2487763.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPVKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(32207..32674)
                     /locus_tag="KPK72_000519"
     CDS             complement(32207..32674)
                     /locus_tag="KPK72_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2487764.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(33060..33536)
                     /locus_tag="KPK72_000520"
     CDS             complement(33060..33536)
                     /locus_tag="KPK72_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2487765.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(33656..34747)
                     /locus_tag="KPK72_000521"
     CDS             complement(33656..34747)
                     /locus_tag="KPK72_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG2487766.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(34740..35129)
                     /locus_tag="KPK72_000522"
     CDS             complement(34740..35129)
                     /locus_tag="KPK72_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487767.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            35321..35767
                     /locus_tag="KPK72_000523"
     CDS             35321..35767
                     /locus_tag="KPK72_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487768.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            36031..36756
                     /locus_tag="KPK72_000524"
     CDS             36031..36756
                     /locus_tag="KPK72_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2487769.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            36743..37987
                     /locus_tag="KPK72_000525"
     CDS             36743..37987
                     /locus_tag="KPK72_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2487770.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSGKYPNLESDIVDIITQGKSMENIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDIKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            38067..38744
                     /locus_tag="KPK72_000526"
     CDS             38067..38744
                     /locus_tag="KPK72_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487771.1"
                     /translation="MEILRVENLTKSYGKNEIKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            38745..41306
                     /locus_tag="KPK72_000527"
     CDS             38745..41306
                     /locus_tag="KPK72_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2487772.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNAEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLFTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            41527..42801
                     /locus_tag="KPK72_000528"
     CDS             41527..42801
                     /locus_tag="KPK72_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2487773.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(42962..46003)
                     /locus_tag="KPK72_000529"
     CDS             complement(42962..46003)
                     /locus_tag="KPK72_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG2487774.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            46209..46508
                     /locus_tag="KPK72_000530"
     CDS             46209..46508
                     /locus_tag="KPK72_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG2487775.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(46746..48974)
                     /locus_tag="KPK72_000531"
     CDS             complement(46746..48974)
                     /locus_tag="KPK72_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG2487776.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYVPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            49267..51042
                     /locus_tag="KPK72_000532"
     CDS             49267..51042
                     /locus_tag="KPK72_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487777.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            51055..>51195
                     /locus_tag="KPK72_000533"
     CDS             51055..>51195
                     /locus_tag="KPK72_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487778.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLV"
BASE COUNT        20488 a         5410 c         8513 g        16784 t
ORIGIN      
        1 aacatgacta tagaaattta actttaaaat aacttatgat aatttagagg aggaatatat
       61 atgaatatat caagaaaagc tatgaaaata atagaattag cacaaaaaat tgcaaataag
      121 agaggtattt cagtagaaga agcttggagt gaagcagtaa cagaatataa aaataaatac
      181 gaacatattg catagatatt taaaaattta gaatatttgg tagaatataa tgattttaaa
      241 tgatactata aattataagg tatatcatta ttaacataga aggaggatat ctgcttgatt
      301 gcaaaaatag ataatacttt agaaaaagaa ttttggcaat atgtatcaca tgaagaaagt
      361 ttaaatttat tcataattgg atacgttgag aattatggat ttagctctca atatcaagat
      421 atatggtctc aggtagaaga tggaaatata acctctataa tattaaaaaa taagtcaaca
      481 cttataattt atagttttaa aaataatttt aatataggag aaatgaagaa tcatataaaa
      541 gatttagatg tagaaagcat aagtggaaaa aaatgtgtaa tacatagact aataagtaaa
      601 tacaaagatt tttatgaaaa actagataat aaattttgtg tattaaaaga aataaaagaa
      661 atagattttt ccaacatgaa ggagtataaa atagaaaatg ctcaggaaaa agatattgat
      721 gaaataggaa aacttttaaa taggtcagac tataaagtaa gtaaaaatta catagaagaa
      781 agaaaagttc atttaaaaga gggaaatgta agagcatatt ttattagaaa tgatgatact
      841 atgattagca ctgtgtcaac tggtatggaa actagttttt tggcaatggt ggtctctgca
      901 agtacagata aaagatatag aggaaaagga cttgcaagtt atatggttta caatttgagt
      961 aaagaattat tgttggaagg aaaagttcca tgtctttttt ataataatga tatagcaggt
     1021 aaaatatatc ataatatagg atacaaagag ataaatgaat ggacaattct ttttaaatag
     1081 ctataaaaaa taaaaaactc tatcgagaat taaagtattt ataaataact atatttttac
     1141 tataattcat gatagagttt ttttaaattt atgatactat tttttattat ttaaataatc
     1201 aagtgtttta agtttataac cttctttttc ccaatgtgta agaacctcat ctaatatctc
     1261 agtatttgtt gatgaatttg gatgcagtaa aactattgca ccaggatgag ttcttgaata
     1321 aattttgttt ttggcaaatt catgagtagg ttgtttcttt tcataccaat ctacataagc
     1381 aaaactccaa aatattgatt tatatcctaa atcttgtgta tattttaaag attgttcgct
     1441 aaatttaccc attggaggtc taaaatactt aggcatttct tttccagtaa catctttgta
     1501 tgctttttct acacttgtaa tttcttcttt gaatttttca aaatctgtaa tacctgccat
     1561 tgatggatgg ctttttgaat gattacaaac taaatgtcct tctttttcca tacgctttat
     1621 taactctgga ttgcttttta tataactttc aactacgaaa aattgagctt tagcattgtg
     1681 ttttttaagc gtatctaata attttggagt gtttccactt tcatatccag catcaaatga
     1741 tagatatata actttttcat tgggatttcc tacataataa gctccgtatt ttttaaaaaa
     1801 atcagcttct tttataggtg atggctgttt accatcttcc ctagggttaa aataccaatc
     1861 atattcatgt gtgtcgagtg ttggactaga aatttgagtt tttgttttat ctaatgattt
     1921 aaaatttata ctagcaattc caaaaagtat tagagcaaat gctcctatca taatgtattt
     1981 ttttaggcta tcttttttca caatttcacc tcttatttaa aatttaaagt atatttctta
     2041 taatatagtt tgaatattta tagttaaaaa tattcacata tacaaataat aaatgaaaga
     2101 agtttactgt aagatataaa gttgatatta attatcttaa ataaagtgta tataaatgat
     2161 aaaatataaa gagaaatgat tgtaaataag ttaggctggt ttaacttgaa gggatataaa
     2221 aatggataat ataataagtg tattgaatga tgttgttttt ttagatatag aagttagtgg
     2281 attagattgt ttaaattcag aaattttaga agttggagct gtcaaggtta aagactggaa
     2341 aatatatacc tatgaatcat taataaaaaa caaatttgag gtacctatag aagtattctc
     2401 agtatgtaaa aatttagaaa aaaatgattt agaaatagct aatgaaatag aactggtgga
     2461 agatagactt gtaaattttg tagaagatag tttcattata tgtcatgatt taagtctgaa
     2521 aaaaaagttt tttgagtatc atatgccaaa gttaaaaaac aaatttattg atttaattga
     2581 attagcagtt attttagaac catatcataa agattatagc ttagaatact taaaaaatac
     2641 tttaacaaat tgtaattcta aagtggaaaa tagagcttta tcagatgcta ttgatataat
     2701 aaatattgta aattgccttt tagtaaaatt taataactat gaaaaaacta ctttggagcc
     2761 attgagtttt aaaataaatt catatcttaa aaaatttaat cttccaacat gggaatggag
     2821 caaattttta gaagaggcaa attatgattt atcaaataat ataaatataa aaaaagaata
     2881 taatatattt gactcaaaag aagaaaagaa gaaggaaaga gaaaccttaa aaattttaaa
     2941 tgaagaagaa aaaaattatg aagaattatt aaaatacaaa actatttggg agaataaaga
     3001 aggatttaca tatgaatata gacctggtca atatgagctt actaaaacaa taagagagtt
     3061 gtttagaaat agcgaagatg aagaaaaaat tgcatgtata gaggcaccaa ctggtatagg
     3121 taaaagtgta ggatatttat tgcctgcaat attagaggct agaattaata aaaaaagatt
     3181 attaatttct actgacacaa aggaattgca gatacaatta ataaataaag atattccaaa
     3241 tgtgttagat tctttgggtc taaatggaaa agtaagttac ggatatataa agggaaaaaa
     3301 taactatatt tgtatagata gactggaagc atatatagat gattatgagt ctcaaaaccc
     3361 tactaaagga gaaattttat ctttaatatt tttgaagaga ttagttgaag gtggcaagta
     3421 tggagatata gaagaaataa attatttagt gtttgacaac ttcaaggaaa taagtactca
     3481 cttaagaaat gtaagttgtg acccaaatat gtgcagacct aaaaaatgca aaaaagattg
     3541 tttatataaa aacagaatag aagaattaaa agaagaacat ataacagtag taaatcattc
     3601 actattagct aaatggccgt ataaggatga aaaaccttta gaaaatataa tagtagatga
     3661 agcacataat ttgactgaaa aaggatatga tttcttttca agcattataa attcaaagtc
     3721 tttaaggtat ttattacaag aaatttatcc ttacgagttt attcaaaata gtagctttat
     3781 atataaaaaa tatagtagaa atatgagaaa aataaaggct tttgataaat tttataatgt
     3841 attaaaaatt ggaagagaag ataaacaaaa aattgctaga agcataaatc tcattattga
     3901 agagatagat tcaatgttaa attttggtaa ttgtaatgaa tataataatg taagtaatta
     3961 taatttaaga tgggaactga atttgcaaat agatgaaatt gtgggaaaac taaaaaagga
     4021 tggtatagat actgaaataa gttatagagc ctatagtgaa aagataaagt tgagttgtga
     4081 gaaaataata aaaaatttag tttcaataat aataataatt tataggaata ttgatgatga
     4141 tagtatagat aaagaagcag atatatataa atttggaaag gcaaagacaa gagatttaga
     4201 ggatataaag attatatttg aaatcttttt agagtatgat gaaaaggatg attatgcaag
     4261 gatagttgaa attgataaga attataatgt ctttgaattt cgagtagtgc cattaaaaat
     4321 agcagattta tttgaagaaa atattttatc tcaattagaa aaaggaatat ttttatcagc
     4381 aactttgagt ttatctgaaa gtatgtctta ttttaaaaat acattaggaa tagacagagt
     4441 taagaatgtg gaaaagatta tagaaccaat ttttgattat aaaaatagag tctctgtagt
     4501 tggattttct gatatttgtg agtacagaaa ttctgaattt ccaaatgaga tgagtaaaat
     4561 tatatctaat atatcaaaga ttacagaagg tcatacatta gctcttttta atagtaaaga
     4621 tagacaagaa aaaacttatg aaatattgaa aaaatactta catagtttta acttagaaat
     4681 ttatgctgat aaaaagggga ttagacacct taacgattta aatagaaaat gtgtagtact
     4741 tggctcaaaa ggatgttttg aaggcgtaga cattccaggg gatggtttgg tttgtgtaac
     4801 tttagacaag cttccgaatc taaatccaaa agaccctttg tattttacta taatgaagaa
     4861 gtatggtata gactactata ctataaatta tcctcaaatg accataaagg taaagcaagc
     4921 tatggggaga attttaagaa gcaaatatga ctatggatgt tttgtgattt ttgatgtagg
     4981 aactaatata agtgtgttaa agagattaga gaaagattta cacgattgta aaatttcaaa
     5041 ggttaatagc aatgagtttt atacttatat aaggcgacat ttaaataaat ctagaagttt
     5101 aatactaaaa tctgttatat ttgatactat aaaagcgttg aatgttgatg ctaaaatgga
     5161 taacaatgat gttgataagg atattataaa aaaagatata aatgaaaata taagacaaag
     5221 agctgtaaaa ggagaagtgt accatattga tattataaaa aaagatatga aagtaaaata
     5281 ttttgataga aactatctta taaatttaga tatatttatg agagaagaag ataaaaatta
     5341 agagtgggag tgatatattt ggcaaaacaa aagttttatg ctgtgaaaaa aggtaaaaat
     5401 ataggtgtat ataatacatg ggatgagtgc aaaaaacaag taaatggatt ctcaggagct
     5461 gaatataaga gtttttctac ttttcaagaa gctaaagaat atattgatgg gtcagaaaaa
     5521 ttgagttttc aagaagataa ggagtttata gaagcatatg tagatggaag ttatgaacat
     5581 tcagtaaaaa tgtatggttc gggtgttgtg attttaaaaa ataatgaagt tataaaaact
     5641 tatagtgaaa aaggaaaaga gaaaacttta gtaagtatga gaaatgtagc tggagagata
     5701 gaggcttcta aaattgcaat gaagtactgt attgacaata atgttcaaaa cttgatttta
     5761 tactttgact atgaagggat tgaaaagtgg tgtactggag tttggaaaac aaataaagaa
     5821 ggaactatag catataaaaa tttctatgac agtattaaga ataagcttaa tgtaaaattt
     5881 acaaaggtta aagctcattc tggaaataag tataatgagg aagctgataa attagctaaa
     5941 aaagctattg gtgtataata aaaaaatatt tatacataat tacatggtat aagagtatat
     6001 ctaaattcct actttataat taaacaactt gtaattaaac aacttataat taaataaatt
     6061 ttgattaaaa aagcagattg tattatgaaa aaataagtat ctaatttata aacaattaat
     6121 aatagtatta attagataca acttatttaa tataataaaa tctgcttttt atataactat
     6181 aaaattttcc tacattctat tagaattgcc cataaatacc ttcaagttct tcttttaata
     6241 cttcagggtc atttatacca atagacatgg ttctagccag tatatttgct tggtcataag
     6301 ttgatgtcca tgagaataca ccacctagac aatttctaag aacatattga gtttttaaga
     6361 atattgataa agcatcatca tatgacattg caaaatctcc atccttaact aaatatggaa
     6421 cttgagcagt attatcaaat ctatattcat atccattttt atttatataa tcccttctaa
     6481 gctcatcata agttcttgtt atagtagcac ctaatcttcc ataaaatggt ataccaagaa
     6541 ggattttttc agaaggcatt ccagcattct caagatttct taccattgca tcaacactat
     6601 atcctggcaa agataagtct gaatcaaaaa gatttgcttg atgttttcta ccatttgggc
     6661 ctgtttcacc tgctgtaaaa tcataactca taagattaaa ataatctatt ataggagcta
     6721 ttttatctat ttcagcactt gaatttatat atcctctatc tcctgttcca gctacactaa
     6781 gccatttatc atctcctata acatctctta tggcagttag taagagtgta aagttttctc
     6841 tatcttgagg tcttgatgtt attccagatg cactacttcc aggatattcc cagtctatat
     6901 ctattccatc taaagcatat tcatttatca tctgattgac ctgtcttgca aaattatatc
     6961 tagatgtagg tgttaatgct gcatcagaga atccttcagc tccccaacct ccaatagcta
     7021 caattacttt taagctaggt ttttcacctt taagagcaag taattgattt aaaaatctag
     7081 gataaggaat aaacaaattt ccagactggt ctatctcagc aaaagcaaat ataactgcat
     7141 ctaatagttg agcatctaca ttagttggat ttccaagaac atattctgct acaattggat
     7201 ttatatctgg acagtttgca ttttcaacta ttggattgta tgggtctgtt atttgtggtc
     7261 taactaattt ttttgaatct tttaaagttt tcatagctat ttttttagta tttttttcgc
     7321 tagtataatc gtagttttta taattatgtt tatagaaatc ttcataacta ctatcttctt
     7381 tgttatagct agaatttatt ttgtcgcaag attcatcttt atcatagtta aaattcattt
     7441 catcacaaga cttatcttta aacttatctt gtttggagtt gtgaactttg ttagtatttt
     7501 tagtatgttt tttctcatgg ttgcaatcat ctttaatatc agtcttgtgt tcttgtttac
     7561 agtcaagagc tttgttcagg tagtcatgat gttcatgttc acagttacat tcattttcaa
     7621 tgtttttagt atgttctttt ttccagctac aagtattatc aattttcttg ctcacttctt
     7681 catcattata attatctggt acaaaacata agtaccagtc catacatgaa tatttcttat
     7741 tacaactgtt aactctattt tttaattcct tatagttttt aggataaggt aaaatcactg
     7801 gcattccagg aaaccagttt gcaggagtaa ctatattatc tctatcacta gtctgaagtg
     7861 catctactat tctaagtatt tctggaatat ttcttcctgt agttagtgga taataaagaa
     7921 tcgttcttag aatttgatta ttatctataa taaatacaga gcgaacagtt gatgtatcac
     7981 tcattggttt tgatatcatg ccgtataact tggcaattct catatctcta tcttctataa
     8041 taggaaatgg aatttctaca cctgtaagta aagaaatatt atacatccaa gctaaatgtg
     8101 aactgttact atcaacactt agaccaatta gttctgtatt tcttttttta aattcgtcat
     8161 aatatttagc aaaacataaa aattctgtag tacaaactgg tgtaaaatca ccaggatgtg
     8221 aaaataaaac aatccaatta cccttatagt cagagagtct aataggacca tttgttgtat
     8281 tggctttaaa atcaggagcc tttgacccta aacttggcaa atttggcatg taaatcaccc
     8341 ctcaataaat tatcatttat tttattctat taattaatac atattagtgt tactatttta
     8401 ttaaaagtta agtaatcctt atgtcaaaaa aagatattta ttaaaaagat ttaatatttt
     8461 cctaatataa atgacaatag taattgctat ttttttacta atttggtata gtaatggata
     8521 aggggtagtg tcaattttag tgattatacg aggtgagtgg attgaaaaaa attctaagaa
     8581 ttttagttat ctccatgatg atatgttttg caatgacttt tgcattagtg ttaataaatt
     8641 cagttatggg agtacatata ggattggatt attacttttt gatgggaagt gtgcttacag
     8701 tttgtttaac aattggaatt gttttatgca atgctagaaa aatatttgat agcaaagcta
     8761 tacacaagac aagaacgaaa actgtcagta ataataaaaa acaaaagagt cattctaggg
     8821 atataaaaag aaagatatca taatttatgt aaaggaaata tttatgtata aaataatgat
     8881 tgataggtaa aataaaaaca cgatttaact tgtgttttta ttttttttca agaattatga
     8941 tataataacc aataagaaat ttagtttacc ataaaatata aagattatta aatggagagg
     9001 gaactataat gaattttaga gatttatttg agaaggctat agattttatt gatgaaaaaa
     9061 gaaaatctat agttgcgatt tcattatcaa cattaggtgt aattgctttg ataatagtat
     9121 ttttcttaag tagtaacgag tttagtgtag gaaatgaagc taatgagttg ttaaagatta
     9181 tagagaaaag acagtattct atagctgttg attactacac aagtatagaa aaaaagtttt
     9241 ctgattcaaa aatggaaagg tttaataagt ctgtttctaa aaaaataaat aaattgctat
     9301 taaatagtgg tgataaatat ttagatggag acataagtca agaaagtttt attggattaa
     9361 taaataccgt gaaagaatta aacaaaataa gtatagatgt ggatgactta ttagttcaag
     9421 ctgatagagt tagagaaatg tataaagaag aaaatactac ttatgatatt gccattaact
     9481 acataagtac tgtttcaata ttaaatggta tgggtagtaa cctagatgta tacaaacaga
     9541 atatagaaac aatcaaagag tctagagatg tttatgactc agcagtaaaa gaccaaaaag
     9601 catataagta ttatgaagct attgaaaagt ataataaagt attaaaagaa gatgaaaagt
     9661 attacagtat ggctcaaaat gggaaagaac aatgtattga agaaatgtat gattactata
     9721 taagtagagc tgaagaggca aatacttctg gagattatga aagagcatta caatatattg
     9781 agtatttaaa agacgattat tcagatgatg aaaaggtaca gagtttagaa aaaaaatata
     9841 aaaagaattt atcattgtat actttaacat ctgatgacat tataaatgta atagctaaaa
     9901 aaagtggcaa agacaaggca aatctatcta taaattcact tccacaaatg ataaaaaata
     9961 ataaatacta ttatgctgaa gtatatgagt atgataaatt aataaatgaa gttttggtaa
    10021 gtgcaaaaac taaagattta tattcatata aagatggtaa aaaagattac aaagtagatt
    10081 atggtgatgg atactttaga atattagaag atggaagtta tcaatttggc attacaaaag
    10141 ataaagctaa gtttgtgtta actaatactc tcgatgaaaa agaaaataag tataaaaaga
    10201 ttgagatatt agatatagag aaagcagata gatatgtaaa aagtaaaaag agtttagaag
    10261 aattgtttgg aaaatataaa aatatttatt attatgctgt agtaaataaa ggcttattta
    10321 gaggcaaaga agtttatgct attaatattt acaatgaaaa aatatatgcc atatcagaaa
    10381 atggactaaa tgaatattaa ttaataatat taaattaata tataattaaa aagtttttgg
    10441 ggttttaagg cgcattatgt agtaatataa taatagggat atataaataa ttttattgta
    10501 aggagaagta tgggatgagt aatgtgaaca aaaagctagt tatagctatt ggagctgtag
    10561 cattagtggt aataattttt ataggcataa tgtgcatgca atttaaaggg gagaaaatag
    10621 caaaaaacac atatgttaat ggagtaaata ttggaaaatt aactaaatca caagcaaaac
    10681 aggaattagc taaaaaatac aaattagaga atgttgattt taattacaat gataaaagtt
    10741 ggaaagtaaa gagcaaagat ttaaatttat ctactgacct ggataaaaca gtagaaaacg
    10801 catataattt aaatagaaag agtgctttct ttggcaatct atcaaaaact atttcagcaa
    10861 attttggcaa aaaaagcaac ttagtagttg tgataaatta tgataaaaat aaattaaaag
    10921 cagaaatgga aaagatagct aaagaaatag atgtagatgt aaaagatgct acacttgata
    10981 taagtggtga aaaagtcaag gttataccag attctgatgg tcttaaaatg gatatttcta
    11041 agagtatgga gaattttgat aatcaaacta aaaaaggtaa ctataaaaat gagttagtag
    11101 taaaagctac tccagcaaaa gtaaaaaaag agcaacttgc taatatagat acaaatttag
    11161 gtacgtattc aacaacattt aaaacttctc aaataaatag aagtataaac ataaaattag
    11221 caacagataa tataagtaat gtattactta tgccaggaga aactttctca tttaataaac
    11281 atacagggaa aagaagtaag gaaaatggat ataaaagtgc acctgtaata atggaaggtg
    11341 agatggaaga agattatggt ggaggagttt gtcaagtttc atctacgtta tacaattctg
    11401 tgttatacgc aggtttagaa attgttaatg taaaaaatca tactatacca tcaagttatg
    11461 ttccaaaagg aagagatgca actgttgcag atagtggaat agatttctta tttaaaaata
    11521 atctaaagca tccagtttac ataaaaaatt acgtttcagg aaatcaaatt gtttgcaata
    11581 tatatggaag tgcagaagat aaacaaaata taacgatatc aacaaaatta gatggcgttt
    11641 cacaaactac aatgaaaaga gttaatgacc ctacaatgcc taaaggtaaa gaaaaagtag
    11701 acaaaagtgg aagaaatgca tattctgtat caacatatag aacatttaat gatgcaaatg
    11761 gtaaaaaaat taaaactgaa aaaatagcta attcatatta tcctaagaag gaaggtatta
    11821 tattagttgg gactatggaa ccaaaaccag aggaaaaacc aaatacagac gaaaataaga
    11881 ataatcaaaa tacaaacaat caaaatccaa ataatcaaca aaaaccagaa acaccaccaa
    11941 ctgataataa gcctaatgag acacaaccac aaccgcaagc ttaaagttct caagaatagg
    12001 atttatgtag agtaataaaa aatataaatc taaataggag ggattacttt gaatttaaat
    12061 aatataacga attacgttca aagtatttgc gagaatattt ctaatgtctt ggatgtagat
    12121 gttactttag ttacaaaaga tttaactagg atagcaggta caggaatatt cagaggtaaa
    12181 ataggagaga caatatcaga gaagtcagtg tactctcttg ttttaaaaga tggaaaatcc
    12241 tatgttataa ataaagaaat agaagagaac tgtaataaat gtgcaaatag agaaaattgt
    12301 aaagagttag ctgatatatg cactcccgtt aaaatgggag agcatatact tggaatcttg
    12361 ggtatagctg cgtttaatga agaacaaaaa gaaaagatac tttcaaaaga taaggagtta
    12421 actgcttttg taaggagtat gtctgattta atatctttta aactagatga actttataaa
    12481 caagaagtta agactataga tgaccttgag agggaagcta tagaaaatgc cataaagaaa
    12541 tatggaagta atacagaagg tatgaaaaaa gtagctgagg cattaaatat aggtatagct
    12601 acactctata gaaaagtaaa aaaattcaac attaaaagtt aatagtattg aaaaagaggt
    12661 aaccttttta tgaaaagaag aggattacct tttttattat gagaatggtg gaaaaatatg
    12721 tatagttttg acttgacata ttaaatatgt tatcttaaat atatatttta gagtggaggt
    12781 gagtattatt ttagttaaag atataaaaca agacatagaa agcatgccta gaaaaatttt
    12841 agatatatct ttgatagaag ataataaaag tgatttgact ttttttaata tagaagaaga
    12901 agacggtgat gaaacattaa aagcattaaa acagagttat gtgactacaa attataaaga
    12961 agaaactcta ttaaacttgc aaggagcttt aaattcaaag tcagacacaa cgtctaagga
    13021 gttaaaatta gaatcatata aaagatataa caatgcattg gatttagcat ataaaaatta
    13081 tataactagt gccattaata tggttaataa agctctggag ataaatccta aagacattga
    13141 tatactaaat cttagaggtc ttttaaaatt attaaaatgt gattttgcta aatcatttga
    13201 gagtttttat actgctttat gttatgaaaa taatcatata tctagaaaat atgtaaatct
    13261 aatttcatct gaagatttta aaactttttt aggaagatac aatcattcta taagatttat
    13321 aaatgaagaa ttaaattatg agtcaataca tattttagag aacataattg aagaagaacc
    13381 tgaactaata gagccatatg taattttatc tttgttatat gataaactag gtaatgtaaa
    13441 aaagagagaa ggatatttag acaaacttaa agagctagat aaagataatc ctgtatttga
    13501 aaaaaatgaa gaagaaaaag aagatacaag taaaaaggaa gaaataaaaa aagtaaaaag
    13561 aaagaaaagt attttgccat atattatagt tggctgtgtt ttaataggta tggggatata
    13621 tttgattcaa agcaaaaaga gaatagaaaa cttgaataac caaataagta gtaaagagga
    13681 aaagttaagt gaaactgata aaaagttagg agaaacgagt aaaaaattag aaaaaacgaa
    13741 taaggaatta aatgaggtta aaaataaaga accagaaaga gagataactg tagctgatga
    13801 ggaagattta tattatcaag ctctaaattt aaagaaaaac aaagaatatg aaaaagccat
    13861 agataatttt aaaagtgtag tttcttctgg taaaactaaa aaatatattt ctgagtcaat
    13921 atatcaatta gcaataacaa ataaattatt aggaaataag gatgaagcta taaaatatta
    13981 caaaaaatat ataaatactt atactaaaaa tgaccagtat tatgatgatt catattacga
    14041 gttgggtatg ctttattatg ataatggcga tttaaaaaat gcacaacaga ctttttatag
    14101 tttgagaagt gaagttccag acagtatgta taacaattca aaaataaaag aaattttaag
    14161 tgaaaaatag gtttatatag ttaagttttt tgaaacaacc aagtatgtat tttattaaat
    14221 taaaataaaa ataaatttaa aaagagattg tgtcaaatta taactttcta ttttaaagaa
    14281 agatatttgc ttgttgataa aactacaaat atatgatttg aagcaatctc tttaattttt
    14341 ggaggtggaa aatgaaagtt ttcaatcaat tagtaataat tttatctatc tgggtaatag
    14401 gagagtatat aagttccttt atacaaggaa ttatacttat tcctgggagt atagttggta
    14461 tgctattatt atttctacta ctacaattta aagttttaga cttgagtagt atagaaaatg
    14521 taagtggatt tttcctcgat aatatggcta tattttttat tccagcaggt gtatcactta
    14581 taaagtcatt agacctaata agggaaaatg tattagtgct tttattagtc atatgtttaa
    14641 gtacactaat agtaatgtat actactggaa ttattgtgga gaaaatgata aaaaagaagg
    14701 aagaaggaca aaaaaatgta taatgtatta cagacaccag tatttggtat aatagtaaca
    14761 atagtttttt tcaatttagg aagatacatt caacgaaaaa caagtaatcc tatatgtaac
    14821 cctttattaa ttgcaatcgt aggaataata ttgtttttaa gcatatctaa aatcccttat
    14881 gagaattaca agataggagc tgatagtcta aatttctttt taggtcctgt cacagtagta
    14941 cttgcagttc cactttataa acaatttgaa ttatttaaaa agcatatgtt tgagattatt
    15001 gtaggaatag gttgtggtat agtgatttct tttgtatctg tattgataat tggcaaaata
    15061 gctaattcag atgtaagtat aataaattca ctaattccaa aatctataac aacaccaatg
    15121 ggaatatctt tagcaaattc tttaaatggt atagaatcaa taacagttgt agccataatt
    15181 tttacaggta tttttggtgg tatggttgct tctacagtat tcaaactggg gaatataaat
    15241 catccagttg ctaaaggaat tgctcttgga acatctgcac atgctttagg aactacaaag
    15301 gcttttgaac ttggagaaat agaaggagct atgagtggtg tttctattgg agtctctggt
    15361 acaattacag taatactaat tccaattata atgaatttta tataaaaaat aaaattaaga
    15421 cgagtacctt gaatacttaa aacaaaataa aataaaaaaa atttaaaata aaaactgaac
    15481 gaaaaaaatg caaataatga aataaaaaaa tcatgtactg aaattataaa aagaaaaatt
    15541 tcatttagga attaattata aagagacaat tactaaactt gaatttgcta aaataaaata
    15601 aattactgcg aaaatagata tttagaaaaa aatactataa atttactata ttgcaagtta
    15661 aataataagc agtatcatat tatgctagaa aaagtttcat ttaaggcata aatgagatga
    15721 tttgaacgtt tttaaagatt agaatataaa agattgcact agacatatat gaaaaagata
    15781 atataataga tgatgtaata gatgacagtt caagaaatta taaaaaaaat ttatttgaaa
    15841 tattatctat tacatcagga ataatatatg taagagtaag cttttaaaat tctaaagatt
    15901 tagaaagatt gaagggagaa agaaatgagt gaagttaaga ataccctaca aaaacatcca
    15961 gttttaagat attcaacaat gctatttggg atgttgatga catctatagg aataaatggg
    16021 ttcttaagac cagcacatct acttagtgga ggtgctacag gtatagcaac atctataaac
    16081 tatttgacaa atataaatgt aggtttatta acatttttaa tcaatattcc aatatttatc
    16141 ttagggttca tataccttga aaaagagttt tgtatttcaa gtttggtaaa tatgattgta
    16201 ttttcattat tattaggagc aactcaggaa ataagtaata ttatacctat acatgatata
    16261 ttgcttcaaa gtgtatatgg tggtatatta tctggtttag gagtcggagt tgtatttaga
    16321 actaggtctt ctcaaggtgg tacagatata atagctgcaa tactaaaaat taagaagaat
    16381 atagaaatga aagacactgc tttagcaata aatggcttga tagtacttac aggaagtttt
    16441 ttatttgggt tggatttagc attatacact cttataggtc tatttctaaa tgcgtattca
    16501 atgagtttta ttaaagatgc aatgaattac caaaaatctg ttatggtaat gtcaaatgaa
    16561 gtcgatttaa tagcagaaga tataatgaag agtttagtta gaggtgtaac ttttttagat
    16621 gcagaaggtg catacactca tcaaaaaaag aagattatat atacaatagt ttcatcaaat
    16681 gagataccaa agataaaaga tatagctttg aagtatgata aaaaggcctt tatatctgta
    16741 aatgatgtta cagaagtaaa aggaagaggg tttaaagcaa aagatttata gaaaatttta
    16801 caagctggga gtttataatg taaatttgta tattatatct ctcagcttat ttaaatttag
    16861 tatacgaaaa taaaggtttt attaatgatt tgtgtatcac tttaaaagat atcattttaa
    16921 aattaaaatt gaaaaaatta cttaatatgt agaaaacatg accactatta ggatataata
    16981 aatttaatag atttagtaaa atatctagga ataacgttgg aggaatggca ttatgaattt
    17041 atctaaagaa tatgttaatt tgtatgataa ttatttgaaa tatataaatg aagtaaacaa
    17101 agagattaga agtataaaga acatgaagaa acgtgtttta aaaaggggcg attttgctcc
    17161 agaaaatgaa gtattagaat tggaaggaaa aacaatacgc gatatagatg atgtcgatac
    17221 aaaaaaacca aaaaataaaa tctataaatt ttcaaatggg gatgtatatg tagggaaatt
    17281 cttagaggga aagatggagg ggaaaggctc atatactttt tttgtagatg aaggaacagt
    17341 tatggagtac ataggagaat ttaaagaaga taaaaaaaat ggaaaaggaa actttatatt
    17401 ttcaaatgga aatgaatata taggttattt tgaggaagat atgatgagcg gtattggaaa
    17461 tatgttatat agtagtaaag atgaatatat tggaacttgg aaaaacggaa aaaaagatgg
    17521 aaaaggtatt tataagtgga gtgatggatg tatatatgca ggtgaattta aaggtggaaa
    17581 gatggaaggg tacggtattt gctataatag taaaggggaa gtactttatg aaggagaatg
    17641 gaagaataat ttgattcatg gtaagggaac ttatatatgg gaaaaaggta aaaagtatat
    17701 aggtgaattt atgcatggta aaaaacatgg gcaaggaact ttttatctaa ataatgaatt
    17761 ggtttatgaa gggacttgga aatttgataa gccatctata tttgatagaa cattagatga
    17821 aatattttcc ttgagactgt agctttatct aattagtaga taaagctttt ttctaatact
    17881 tactgtaaat aggtatgaat gatattgatt agtatacact atcaatatta acttaataaa
    17941 tgcaatatta aaaaattttg attgatttta agaaggagag atatattgaa gagattaatt
    18001 gctttagtgt tagtagtact tattgtttta ttttatagtc ctgaattatt aaaagaagga
    18061 tatgatttaa aagaaaagtt ttttcaaagt gaaatatgga atgaattttt agacaaaata
    18121 aatataaatg ataattctaa gaaagataaa aaagaaaaaa gtcaaaattt acaatcaaat
    18181 aatactgaag gggagaactt aggagagtca gatataaaga attttgaaaa aataagctta
    18241 ggagataatt taagctatgt tttatcaagt attggtaaac ctggtagaat tgatattagt
    18301 gaatatggtt ttgattggta cgtatataat caatatggaa aagaatttgc aatggttgga
    18361 cttgaaaatg atgaagtagt agcactttat agtaattcta ttaattcatg tgaaaatcaa
    18421 gatataaaac taaatcaaga tagacaaaca gtgagaacta agattacacc attaaaatat
    18481 aagagaaaag gaaataccag atatataata aactcagaaa atcaatatga tataataagt
    18541 aaggaaggta agtatattac aattttctac gatatttatg aagaaaatag agtctgttca
    18601 tatttaataa ttgataaaag cactgaagat gagtttaaaa atttatatcc agatgatagc
    18661 gaagaactta aaaaatgttt tgaacttgaa gttatagacc ttgtaaatag tgttagaaat
    18721 caaagagggt taaactcatt gagatacagt gaacaggcaa ctttgagttc aagaaaacat
    18781 agtgaagata tgagagataa taattttttt gaccatgtaa ataagaagaa tgaaactcca
    18841 tttgacagaa tgaaaagaga aggtatagta tatacttcag ctggagaaaa catagcagca
    18901 ggacagatta atgcaatata tgcgcatgaa gcttggatga actctgaagg acatagaaaa
    18961 aatattttgg ggaactataa taatattgga gttggagtaa tatttggagg aagctataaa
    19021 acatattaca ctcaaaattt ttataaataa ctaaaatatt tgaaaggaag ttaaaaaatg
    19081 acatcatgga aaaaaaagac agtatataag tgcttaatag cagtagtctt gttttgtgga
    19141 atagtgttaa tatctaattt tagtaaagtt tctgctttga tgatggatac taatggaaat
    19201 gttttgatta aacatggttc aagggaaaaa aagttaatag caattacctt tgatgatggt
    19261 cctcatccta aagaaactag tcaagtttta gatgtattaa agaaatacaa tgtaaaagct
    19321 acttttttta tagctggaaa acatgcaaaa tggtataaag aaccattagt aagagcaagt
    19381 aaagaaggtc acgagatagg aaatcatact ttcaatcatc ctgatataag taatttaagt
    19441 tctagtcaga tagaagagga gattgttaag tgtgaagaca ttttgaagga agtaactgga
    19501 aaaaaaccga cattatttag accaccattt ggaagttata gagaaaaaga attaatagaa
    19561 attgcaaaaa agcatgacta taaagttgtt ttatggacag gtgtagatgt gaaagattgg
    19621 aaaaatccag gagctaatag cattgcagat aaaataatta ataaagtaca aaatggagat
    19681 ataatacttt tgcatgatta tgcaactaat gatacagttg aagctttaga tatgtttatt
    19741 ccaaaaatga ttgaaaaagg ttttaaattt gtaacagttt ctgagttgat aaaataattt
    19801 ttagggggga gatttacagt atgattctta tgatagacaa ttatgattct tttgtataca
    19861 acctagtcca atatatagaa gaattagggg aaacagttgt agtaaaaaga aataatgaaa
    19921 tcaaaataag tgacattgaa gaactaaatc cagaggtaat tgtgttatct ccaggaccgt
    19981 gttctccaaa agaagctggt atttgcatag atattgttga gcattttaag gggaaaaaac
    20041 ctatactagg aatttgttta ggtcaccaaa ctattggaca tgtttttgga ggagatatta
    20101 taaaagcaca acagcctgtg catggaaaag tatatagtat caaccatact aataaaggag
    20161 tttttagggg acttaaaaat cctttaaatg ttactaggta tcattcacta atagttgact
    20221 ccaatacagt tccaaaagaa ttagaaatta cagcaattac tgataaaggg gaaataatgg
    20281 ggattaggca taagaaatat ttaatagaag gagtacagtt tcatccagaa gctattttgt
    20341 cagagtatgg tcatgaaatg cttaaaaatt ttataacaga agctagagaa agggtacata
    20401 tgtaatatga taagagagat aaatactaaa ttaaattctt ttgagatatt tactattttt
    20461 agaaatgaac atgacagttt tatattagac agtgctatgg ataaagaaaa attgggaaga
    20521 tattctttta taagtagcca accatttaag gtattaaagt ataaagatac tgatgaaaat
    20581 cctttagaag ttttaaaaga agaactacat aagtataggg ttgtaaatga tactaatctt
    20641 ccatttgtag gaggtgctgt gggatattta tcttatgatt taggtaatta catagagaat
    20701 ttacctagaa ctgctgtaga tgatattgag atgcctgata tgtattttgg attttataat
    20761 catgtaatag ttatagacca tcttgtgcaa aaaacttata tagctactcc aaacatagat
    20821 atcgaattag aagaaaagat aatagatgat atagaacaaa gaatattaaa agaagagaaa
    20881 aaaggtatag atagcatatg ttatgaagaa aaagaagtaa cacctataag acttaaatct
    20941 aattttacaa aggaagaatt taaaaatgca gttcaaagtg ttagagagta cataagacaa
    21001 ggagatatat atcaagctaa tttaacacaa aggttcagtg gagaaacaga acttacaagc
    21061 tttgaattgt atagggactt aagaagattt agcccggcac catttggggc attcttaaac
    21121 tttgaagata cacatatatt atcaaattct ccagaaagat ttataagatg tgttaataag
    21181 agaatagaaa ctagaccaat aaaaggtact cgtccaagag ggaaagataa agaagaagat
    21241 ttaagacttc aacaagaact tagaaatagt gaaaaagata gagctgaact acttatgata
    21301 gtagatttag aaagaaatga tattggcaga atatctaaaa ctggaagtgt taaagttcca
    21361 gagctatttg taattgaacc atatgcaaat gtaaatcact tagtttccac agttgtaggt
    21421 gagttaaaag atgataaaga tgctactgat gtaataaaag caacttttcc aggggggtct
    21481 ataacaggag cacctaagat tagagcaatg gaaattatag acgaattaga gccaacgcaa
    21541 agaaatgtat atactggttc aataggttat ataggtttta atggtgatat ggattttaat
    21601 atagcaatta gaacaataat caaaaatgac aaaaaagtct actttcaagt tggaggagga
    21661 atgacttggg attctgaccc agatgaagaa tatcaagaaa cacttgataa agctaaatcc
    21721 ataatgaagg ctttgagggg atattatgaa gaataatgta ggttttgata gtagtttaag
    21781 taaatttgga attggtttgt ttgaaactat tagagttaaa aatggaatag cagtagattt
    21841 agatatccat atagaaagaa tgctaagttc aataaattgt ttggatttag atataaacta
    21901 tacaaaagat ttcttgataa atgaaattgt tacatatata aaaaaagaaa atgtcattaa
    21961 taaagcactt agaattactg tttttgatga aggttataat atatctatta gggatattcc
    22021 atataatcaa gagacgtatg agaaggggtt taaattagct atatctccaa tagttagggg
    22081 gaatagttta attcatagac ataagactac taattatttt gaaaacatat ataccaaaaa
    22141 tatcgcaaat aagactggtt ataatgatgg tatattttta aattcagaag gtgtaatatt
    22201 agaatgttct atgagcaata ttttctttgt aaaaggagaa agagtgtaca ctcctagtga
    22261 tagattaccg atattaaatg gaataataaa aaaaaggatt atagagattt gtgacgaatt
    22321 acatatagag ttgatagaaa atgaaattaa tatatcagaa attagtagtt ttgattttgt
    22381 ttttgtcaca aatagtctta tgggcgctat aaaggttaca gaaattgaca aaataaaatt
    22441 taataaagaa aatgttgttt ttgataaaat agtgaagtta ttagaataaa ttaaaatatt
    22501 aacaatttgt tggagacaaa agatgatagg ttgtatttat gaagtataca atgaagagat
    22561 aactaaaata atagaaaacc aaaacacttt ggctgtatat ttagtgggtt ctagtaaaga
    22621 cgtagatttt actctttatg atgtagagat aaacgatatt gatgtgttcg tttttgtaaa
    22681 agaaggggaa aaacaagaga gaatactgtt taaaaaaaag gaaatagaat ttgatgtaaa
    22741 ttacttttca aaagatggtg taaaaaaatt attatcaaat agggaatatt tctttgtgaa
    22801 agaaatgaaa gatgctaaag tactgtttga tagggagaat atatcacata ttataaaaga
    22861 tataagtaga gatatttact tagaaggacc tagtaaaata tcattagagg aaaagtcttt
    22921 tataaagcaa gatattggag caaaaatatc gaatttaaaa aataaagaaa aatttgatgt
    22981 ttttgaatat cattttttga cgaatttata tttaaaagat ataataattg ggtactttaa
    23041 tataaatgat aaatgggtac caaaagacaa aaaattgcta aaagttttaa aaaaagaaaa
    23101 taatgagctt tttgaactag tgtcaaaagt aagtgaaaat tatgactatc aaagactttt
    23161 agatgtttat aattatattt ttaaagaaat tgagacaaaa gaggttataa aactaatctt
    23221 ctagtcacaa atcaatgggg ggagtttaaa tgaataaagt tgataaagaa aagatacaac
    23281 atgcagtaag agaaatacta gaggcaattg gggaagaccc agatagagaa ggtctaatcg
    23341 aaactccaaa tagagttgct aggatgtatg aagagatatt ctctggtctt agtgaagaac
    23401 caagagacca tttaaaagtt ctttttgcag atgaaaaaca tgaagagtta gtattggtaa
    23461 aagatattcc attttattca tgttgtgagc atcatctagt tcctttcttt ggaaaagctc
    23521 atattgctta tttaccaaaa ggaggaagac ttacagggct ttctaaactt gctagagtaa
    23581 tagatacatt ggcaaaaaga ccacaattac aagaaagaat cactaaaaat gctgcagata
    23641 taataatgga agaattacaa ccttatggtg tcttagtagt agtagaagct gaacatatgt
    23701 gcatgacaat gagaggcgta aaaaaaccag gttcaaaaac tgttacatca gcagtgagag
    23761 gaatatttga aaaggacatt gcgtctagag ccgaggctat gagtttgatt actatgaagt
    23821 agcaatttat tatagatata atcatttatt gagataaaat ttaatttaag ggagtgtcat
    23881 taattgtgtt tgattatggt aaaagaactt atataatggg aatattaaat gtaactccag
    23941 atagtttttc tgatggagga gattttaata acttggacat agcaatacaa catgcaaaag
    24001 atatggttga ccaaggtgca gatataatag acctaggagg agagtctaca cgcccaggtc
    24061 atagctatgt agactcagac gaagagctaa gaagagtaat ccctgtaata aaaaaactta
    24121 aacaggaatt ggatatacca atatcaatag acacttataa ggcagatgtt gcagaagaag
    24181 cattaaaatt aggtgtcact atggtaaatg atgtttgggg tcttagaaaa gataaaaata
    24241 tggcttctgt aataggaaaa tatgatgcag aagtgtgtat aatgcataat caagatggga
    24301 caaactatga caaagatata atggagtcaa taaaagactt ttttaaggta agtatagaaa
    24361 tggcaatgag ttgtggtgta aaaaaagaaa aaatagtact tgacccaggt gtaggatttg
    24421 gcaaggattt tgaacaaaat attgaagtat tgagaagatt aaatgaatta aaagatttag
    24481 ggtatcctat tttgcttggg acatccagaa aatcagtaat aggaaaagta cttccagttg
    24541 agcctaaaaa aagattagaa ggtacaatag ctactactgt tcttggtata agagatggcg
    24601 tagatatagt tagagtacat gatgtttatg aaaatttaat ggcagctaga atgactgatg
    24661 caatatatag aaagtaggta taatataatg gataaaatat tacttagtaa tctaggattc
    24721 tatggatatc atggtgttct taaagaagaa aattttctag gtcagaaatt ctttgtcgac
    24781 atggaacttt atattgattc aagagaagca ggtcttagtg acgatataaa taaatctgta
    24841 agctatgccg aggtttacaa tgttgttaaa gatattactg aaaataaaca atttaatctt
    24901 ttggaggctt tagcggaaaa tattgctgaa gaagtcttga ataaatttat acttataaat
    24961 ggagttatgg tcagagttag aaagcctgag gctcctgtaa atggtattta tgactatttt
    25021 ggtgttgaga taaggagaac aagagatgaa taaggcttat ttgggaatag gtactaacat
    25081 gggtgataga tttgataatt tatcaagagc atgtgaactt ttgaaaaata gtgattctat
    25141 atataaagta aaagaatcaa gcttatacga aacaaaacca tggggatata cagaacaagc
    25201 agattttcta aatatgtgtg tagaaataga aacagagttt gaaccttatg agttgttaga
    25261 atattgtcaa gaaatagaga gagaactaca cagagagaga atagttcatt ggggaccaag
    25321 aactatagat gtagacgtac tattttttaa tgatgtagtt tccactgatg aaagactaac
    25381 aatacctcat ccaaggattc aagatagagc tttcgttttg ataccactta tggatttaaa
    25441 tgaagaactt ataataaatg aaaaaacaat aaaagaacat ttaaacctcc tatctgctga
    25501 agaaagagaa gaagtaaagg agcttgtagg ttatgaaaga aaacctattt aataataatt
    25561 caaagataga gataaataca aataacaaaa agataactgt aaaaaaagat aaacttataa
    25621 tagctggacc atgcgcaata gagagttatg agcaactttt ggagactgct aaatttgtaa
    25681 aaagtcaggg agcaaacatt ttaagaggtg gagcatacaa gccaagaaca tcgccaaatt
    25741 catttcaagg attaaaaaaa gaaggtcttg aaatattaaa agcggttaaa gacgaagttg
    25801 gtatggctgt tattacagaa cttatggatg taagagatat ggacgaactg tatagtataa
    25861 gtgatataat tcaaattggg tcaagaaata tgcaaaactt tactctttta agtgaagtgg
    25921 gaaaacaaaa taaacctgtt atgttaaaaa gaggaatagc atccacaatt actgaatgga
    25981 taggtgcttc tgaatatata gcaatagaag gaaatagtaa tattataatg tgtgagagag
    26041 gtataagaac gtataatgat tatactagaa atacattaga tttagctgcg gtacccatta
    26101 ttcaaaagga aacaggtctt cctgttgtgg tagacccaag ccatgccact ggagttagat
    26161 atttggtaaa acctatgtca ctggcatctt ttgcatgtgg agcagatgga ataatggtag
    26221 aagtacatcc tgacccagaa aatgctttgt cagatggtgc tcaatctctt tgctttaatg
    26281 aatttgaaga tttaatgaaa tcaattaata attattaaaa gaggcaagag cctctttttt
    26341 attcgtaatc gttgctaagg taaatttata agttataatt tgatagttag attatttaat
    26401 ataaataaag atttaatatt gatagaatta atataaaaaa taaaaatttt ttgattaata
    26461 agaaggatat caataaaaaa agtcgaattg tgtatattga gtgattgaca aatttagaat
    26521 atagtgtaga atagaaatgt taacacaaaa aattacacca gaaggtgata atatgaatga
    26581 tataaaagac attattgaag aaataaaagc tagatgcgat atagcaagta taatatctga
    26641 atatatgaat ataaaacaat ctggagctaa ttacaaagga ttatgtccat ttcatggcga
    26701 aaagacacct tctttttata taaatacatc aaaacagatt tataagtgtt ttggttgtgg
    26761 tgaaggtgga gacgtaataa attttgtcat gaaaatggaa aatttggatt ttatggatgc
    26821 tgtaaaaatt ttagcaaata aatgcggtat agaaattaac actaacatga atgaagaaac
    26881 tagaattaaa atagaaaaat ctaagaaatt tcaagatatt catacggaag cagctagatt
    26941 ttatctttca aatttattgg gaagcaaaaa tcttgggtat gaatatttaa gaataagagg
    27001 actagatgat aaaattatta agaaatttgg tttaggattt tctttggatt catggaattc
    27061 acttatgaat actctaatca gtaaaggata taaaaaacaa gacttattgg agtgcggtct
    27121 aattgcaaaa aatagagatg gaactaattg ctatgataaa tttagaaata gagttatgtt
    27181 tcctatattc gattatagag gaaatataat aggatttggg ggaagagtat tagatgattc
    27241 tttgccaaaa tacttaaatt ctccagacac attaattttt aataaaaaac agaacttata
    27301 cggattaaat tttgctagaa aaaatttaga aagtaaaact atagtcttag ttgaaggata
    27361 tatggattta atttcgcttt atcagtatgg tataaaaaat gtagtagcaa cccttggaac
    27421 agccttgact gaacaacaag gcattttaat aaagagatat gcggacactg ctattatatc
    27481 ttatgattct gatgaagcag gaataaaagc gactttaaga gctatagata tacttactaa
    27541 attaggtatc aatgtaaaag tgttagactt aaaagatgct aaagacccag atgaatttgt
    27601 aagaaaatac ggacttagtg attataaaaa agctatggat gtttctactc actacataaa
    27661 atataaaata gatcatctta aaaaagaatt taacattcaa aaagatgaag aacgagtgaa
    27721 gttcgcaaaa gaagcttcta aaattataaa acaattgaca agtcctgttg aaattgattt
    27781 ttatacaaag tatttaagca atcaaataga tattaatgtc gagtctataa agagagaagt
    27841 ttatggtaaa aattacaata agccatataa taacaaaaat caaaagaaaa tagaagaaaa
    27901 agtaattgaa aaagtcgaag tcagacaaga cggaaaacaa ttagttgaag aaactctaat
    27961 aaaaataatg ttagaagaca aaaaaattag agaaatagcc ttacttaaag tagaagaaag
    28021 tgatttttta ttgaaagaaa gtaaagaaat tttaaattac atgattaaaa atcaagaatt
    28081 ggacaaaata actattgaca aattgaaaag tttaaacata tccgaagaat acttaaaaga
    28141 attaaattca atttctttga atagtataaa cttagagaac acaaaagaaa tagaaggaat
    28201 aataacaaat atcagaaaaa actcattaga agagcaaatt aatagcttgt tgagagaaca
    28261 acaagaatta gaaaataata atgatatgaa agaggtagat ggtagagtta tggaaattgc
    28321 tttaaaaata gttgagataa ataaaatttt aaagagcttg tagagaaagg aggtttgagt
    28381 gtggaaaata aatcgaataa aaaagagtta aaaaaggtta ctgctaagac attaatagaa
    28441 aaaggaaaaa agcaaggttc tctgacactt gcagagataa tggaagcttt ttcggagact
    28501 gaacttgata aggatcaagt agaaaatctt tatgagactt taggaaattt gggaatagaa
    28561 ataacagaaa caaaaaacta taaagctgat atagattttt cggttgctga tgacgattta
    28621 agtataggcc acttagatga agatgcagag gcaatttcac atgatgactc ttctgcaata
    28681 gaaatagaaa ctgtggattt atctttacca aaagggataa gtatagatga ccctgtaaga
    28741 atgtacttaa aagaaatagg aaaaattcct ctacttaaac cacatgaaga agtagaattt
    28801 gctagaagga tgcacgaagg tgatgagata gcaaaacaaa ggttagttga agcaaactta
    28861 agactagttg taagtatagc aaaaagatat gtaggaagag gtatgctttt cttggattta
    28921 atacaagagg gaaatttagg tcttataaaa gcagtcgaaa aatttgacta tacaaaagga
    28981 tataagttta gtacttatgc aacatggtgg ataagacaag ctataacacg tgctattgca
    29041 gaccaagcta gaactataag aataccagtt catatggtag agactataaa taagctaata
    29101 agagtatcaa gacaactact tcaagaactt ggaagagatc caaaaccaga agaaattgca
    29161 aaagaaatgg aaatgacaga agataaagta agagaaataa tgaaaatagc tcaagaccct
    29221 gtgtctttag aaacaccaat aggagaagaa gaagatagcc atttaggcga ctttattcca
    29281 gatgatgatg ctccagcccc agcagaggca gcagcatatt ctttattaaa agaacaaata
    29341 gaagatgtac ttggttcatt aaatgataga gaacaaaaag tattaaagct tagatttggt
    29401 cttgaggatg gtagagccag aactcttgag gaagttggaa aagagtttga tgtaactaga
    29461 gagagaataa gacagataga agcaaaagca ctaagaaaac taagacatcc aagtagatca
    29521 aaaaaactta gagattattt agactaaaaa gatacatcat tgatgttttt gtgataaatg
    29581 ccatgagata agtgatatct catggctatt ttgtagtaaa gatttaattt tatttttgta
    29641 gttatttcag tctaatatct ataacttgga ttaaatccta taaataaagg gaattgaaag
    29701 gagaattata tttgaaatta acagatagat tattgaaaat agcatcatta gtgtcagatg
    29761 gtaaaaaaat agctgatata gggacggacc acggatacat accagtatat ttgttaaaag
    29821 agggaaaagt accatttgca gtgctagcgg atgttaataa aggacctctt gacaatgcac
    29881 ataaagaagt catacagaac aatcttttag ataaagttga tttacgattg ggttctggta
    29941 tagagatact tgaaataggg gaagtagaag aagtgataat tgctggtatg ggtggaatct
    30001 tgataagtga gcttctcgaa gctaaaaaag aagtagcaca taatgtagaa aaattaatat
    30061 tacagccaat gcaagcacaa gaagaactta gatattacct tttaaacaac ggatatgaaa
    30121 ttttagaaga agttttagtg agagaagatt ttagaatata tgagataatt gttgccaaat
    30181 atacgggtaa aaatactata atagaagatg agatatatta tgaggttgga ataaagcttt
    30241 tagaaaacaa agattctctt tttaatgatt ttatagaaaa aaagataaag acatatagtt
    30301 ctatagttaa taaattagaa gggaaaaatg gggaagctat agataagaag agaaaggaaa
    30361 gcgaagttgc aataaaaaaa cttgaaaatt taataaaata aataggaggt atatatgcta
    30421 ttaaagagtc taactagaaa aattgaaaaa aagtatcctc taaacttagc agaggattgg
    30481 gataatgtag gtcttatagt tggagatttt gatatggatg taaaaaaggt attggtatct
    30541 ttagaagcta atgaagatgt tattgatgaa gctatatcca aaaatataga tttaattgtg
    30601 acacatcatc cttttatatt tgggaaaatt aataaaatta atagtggaga tttaaaaggc
    30661 agacttatac aaaaacttat taaaaatgat atatcacttt attctatgca tacgaatttt
    30721 gatatagcat ttgatggtct aaatgactat tttatggaaa ttatggaatt tggtaattca
    30781 aaggttttag atataactaa aagcgaaaat ttgtataaat tagcagtata cgttccacat
    30841 aactattcag atgaacttag aaaggtttta agtaattcag gagcaggtca tatagggaat
    30901 tatagtgatt gtacgttttc aatagaaggt gaaggacaat ttaagccatt agaaggctct
    30961 aatccgtttc ttggaagtgt aaatgatata gaaactgtaa atgaagttaa aattgaaact
    31021 gtagtaccac agaagctttt aggtggggta ataagttcta tgttagatgc acatccttat
    31081 gaagaagttg catatgattt gtacaagtta gaaaataaag gtgagatatt tggactaggt
    31141 agaataagta aacttgataa aagtatgaca ttagaaagtc tttcaaggaa aataaaagag
    31201 aaattaaata tgaaacacat aagagtagtt ggaaacctta gtacagacat aacgaaagta
    31261 gccgttgtaa caggagctgg ttcagaattt gtaaaaaaag ctaagagaca aggtgcagaa
    31321 gtactaatta cgggtgatgt aaagtatcat gaggcacaag atgcacttga tattggaatg
    31381 tgtatagtgg attgtggtca ttttgataca gaagatatat tcaaaaatgt tatgaagaga
    31441 tttttagatg aatttagtga aattgaagta ataaagagta atgtatattt gaatccattt
    31501 agtactattt agcagtgttt gataataaaa atactatata aaaaataaat ttgagtttaa
    31561 ttagggggat aatgatgaaa gtagcagtaa tttttcatag tgtttgtggg agtacatatt
    31621 tattagctag agaatataaa gaagcgcttg aagaaatgaa tatcgaggta gatatattta
    31681 gagtaagtga cgaagttgca aaaacacttc ctcaatatta tttgataaat tccaaagaat
    31741 acaaagacga atttgaaagt ataaatgtaa ttaaatctgg aaaagaaata ttagattatg
    31801 atgctatatt tatgggttca ccaacatatt atggaaatgt atctggacca gtgaagatgt
    31861 ttatggatag tttttctgat atttgggtag gtgctccttt gagtggtaaa atatttggtt
    31921 gttttgctac agcaggttca caacatggag gaggagaatt agctttgcaa gctatgaata
    31981 tatttgccca acatatggga atgactcttt tgtcagttcc atgttcagta agaggaggtt
    32041 atccagcgta tggaatactt catatagcag gagataattc tgatataaga ccaaatgatg
    32101 atgccaagat aggtataaga gattacctaa aaagacttaa catataagaa tatcatataa
    32161 aaatacctaa aagcgactgt atatactttt aggtattttt acttaattat aaacgtgaag
    32221 ctttaccaga aatatggtca atttctattt taacaacagt tgttaagttc atatccttct
    32281 ctatgtactt catgccttct tcaaagaatc ctttagaata tttttttatt atttctacaa
    32341 gagcattttt cttttcttca ttttctacag tgcaagcttt tccaaaaact atagcactag
    32401 aataagtagt actaaattta tctggaataa cactttcatt agcaactact aaaaaagaaa
    32461 ctttgttgtt tttcgatata ttatctaact tatgcccatt tcttgcacaa tgaaagtaaa
    32521 tagaatcatt aaagtataca taatttacag caactccata aggataacca ttttcagata
    32581 tagtggaaaa agtaccaaat tcaccatttt ttaatacttc aacagtatct tcttttgtca
    32641 tttctctttt tttcaatctc atttctttaa acataatatc atcctttcta attttgatgc
    32701 ttaaattaaa ttttagaaca tttataaaga ttatgctatg ttttttacaa attttaagaa
    32761 aaaaatatag tttaagataa aaggtagtga aagagaagct atttggaggt ttatttatga
    32821 tattattgtt agagtagtat aaacaatact aataaattta atagctggtt tcttgttatt
    32881 taagatagta agcatgtcta taagaagggg aagttatcga gggtagaagt tgttatttat
    32941 aactatatta taaagaaaat attattatat aaaataataa atagtaaaga ggtatcaagt
    33001 tgaatataat acttaatacc tcttcaaaat taagttataa aagtgttgtg ggaattgtcc
    33061 tatacatggt aaggagataa taaagaatgt atctttaaag tctcaactaa acagcaaaat
    33121 ttatccaaca aacatacaat aaatgcttct ttgtatctag gtggcttaac agtaagtggc
    33181 cacatgtgtt ttaaaattat gtccgtttct ttttcattta tttgaaaaaa taaagttgca
    33241 ttttttaatg cttctctagg gtgagtaaat ccatgtaatc cttttctatc acctttgtaa
    33301 tgccaatcat aaagaaaaaa gtcgtgtagt aaagctcctc taataacact ttgaatatca
    33361 acacttaagt gcaatttttt acataataga taactataat atgctactga caaactatgt
    33421 tcaagacatg aaacattgcc atgatgagga aatgttctca taagttgaac tttttcagaa
    33481 gaaagtatct cagaagcaca ttcattaaaa tatttgattt cagcttttga caacattatg
    33541 aaaatctcca ttcttagtaa tatataaata ctatacttca acgataatat gaaatatttc
    33601 ttatcctaca aaatataata cccaataata caagtaagat accttttatg gtattttaaa
    33661 gtaagagaga ttttattagg ctaaacaatg aaaaaattaa aagtaaagct atagcaatat
    33721 atagtatttt tccaagagga ttgccaaaat ttatagtcca tccaatacca aaccttttat
    33781 taaccattaa tgatgggtca tttggattat tatacatgat gcctgctatc caatacttct
    33841 catcttcttc tggagaataa gatgagttga attttaaatt tggggaatta atatatgttg
    33901 ctattaaata aattataact aaaagcgttg taatattcat agcagttgat agttttgttt
    33961 ttattgataa aagagtagta aaaaactgtg ttgtcattat aatcatcaat attaaaaaca
    34021 aataaccaat tttatttaaa tatttaatgt tttcaactct actttcttca attctatttg
    34081 tatctatctt aatccttgac tttatggatc caatggaagt tatatataat aaaaccatta
    34141 aacaaaagtc agtaccgatt aatttaaata cattaagaaa tgatttatct ataaaaacat
    34201 caggagaccc atttatgttc caatgagtgg caattgagtc tggcaattga ttataattaa
    34261 atgctataaa aatactcatt cccaaagtaa atagtatcgg tattaaatag attattttaa
    34321 atttttttat tattttattt ttcttattta taaaatccat atctactatt aattttgaat
    34381 ctatatctat atgacctaat ttagaataaa tttctgattt aagcttctta gcttttttat
    34441 gtgtatgaat atatagtata ctttcatata ataaaaaacc tataatacca ataaaatagg
    34501 aaagttccaa tttattaaat gtaaagacca gaataaaaac taatataaag tctataataa
    34561 aacctaaaga taatagtttt ttaaattctt tatctaactt tataaagtaa ttataatttt
    34621 tatgctcttg gtctatagaa actccataga tgtgtttttt acctacaaaa tagggcataa
    34681 aataaaatgt agcatacatc aaaattagaa ctggaaatat tgtaaataga cattcaatct
    34741 tactcataat atttcacctc atacttgtca aataaacttg aacttagtga caaaaattct
    34801 tctttactaa tacctgataa ataagatttt gccaccaata attccaagtc aagtttattt
    34861 ttttgacgag ttgtattatc tattttcatt ggtagttgag ctacaatagc accttttctt
    34921 ctatcaatat ttaagaatcc ctcttctttt aattcattgt aggatttgtt cactgtatgt
    34981 aaatttacac caatattttc agccatactt cttacagatg gaagtgattc atttattttc
    35041 aattctcctg aagctatagc cttagttatt tcttctttaa tttgtacata aatagattta
    35101 tcactattaa aatctaaatt taaaaccata aaaaacctcc attatgcttt ataaatgaaa
    35161 acgattgtta tatacatagt ataacataaa aaataataac aaacacaaaa tataagttta
    35221 tattttgtaa aaattgtaat ttaaaaaata acataatttt tattaaaata atacagatac
    35281 tatttataga tagaaataaa aattagaaag gagaaaaaat atgttaacat atttaatgaa
    35341 aagacaaatg cataaaaaag atgatggaat gagtaaacca atgatgatta caggagctgt
    35401 aattacaggt gtaggagcat atgctgtata taaaatggta aaaagccata aatctaattc
    35461 tcaaatggtc aatacaaatt attatggaaa tagcgattat gatgattata aatatgacga
    35521 atttgattat gactgtgatt gtagagacac aaaacaagat tatgaatatg aagaattaaa
    35581 aaaaaaagta aaagaattta attcaagaag aataaattgt gaaaattgtc cacatgtttc
    35641 tcaagaagaa atgatgcaat atgtaaatag tgttaaagat gtaaaggaag agattgattt
    35701 acatgaagat gctaaaaaag ataatattta caaagaagat gaatacaaaa aatatgaaga
    35761 agaataagta atattttgta taactgaaca aatagtaatg tcaaattatt taatatctag
    35821 ttttaatatg taagttgtaa cttaacttga aagaatcagt atcattcttt agatagttaa
    35881 gtaacaactt acattttatt tttataaatt ttaatagttc tttgtgacta ttttgtaatt
    35941 ttagaaatat tttagtatcc agggtaattg tgtataataa aacttgtata gatagatagt
    36001 atgaaattaa gcagatagtt taaaattaaa atgaaaagaa aggaaatagt tatgttaaag
    36061 ttgttgatag ttgaagatga tagtactata tcttttggaa taaagtatgc tttagaacaa
    36121 gaaggattta gtatagatat atcaaaggat ttatctagtg gaaaagaaat gatttcttct
    36181 aatgaatata gcatgatact gttggatgtt acattgccag atggaactgg ttatgagctt
    36241 tgtcaatata taagaggatt ctctcaggtt cctataatat tcttgactgc ttgtgatgaa
    36301 gaagtaaata ttgtaatggg tctcgatata ggtggagatg attatataac aaaacctttt
    36361 agaattagag aattaatatc cagaataaaa gctgttttaa gaagaaaagg taatacttca
    36421 gaagaaaata agaaaatact taaatttgga gatttaagta tatatacatt agaagctaga
    36481 gtatataaaa atgataaaga aatatttctg acatcagttg aatataaact tcttttaatt
    36541 cttatacaga ataaaaatac tgttttaagt agaactcaaa tattggaaaa actttgggat
    36601 gtaacctatg attttgtaaa cgacaatact ctcactgttt atataaaaag gcttagagaa
    36661 aaaataggtg atgatttatg tgaaccaatc tacatagaaa ctgtaagggg gattggttat
    36721 aaatggatag gaagtgaaaa tagtgtttct atataatcca gaagtgaaga agtttttaag
    36781 taagtatgtt actttgatgt ttattgttat cataatatct attggattta gcataatcaa
    36841 cgtatcttta actaaagaga tggttgtaag aaataatcaa gctataatag ggactttatc
    36901 gggtaagtat ccaaacttag aaagtgatat agtagatata ataactcaag ggaagtctat
    36961 ggagaacata gattatggtc agaaaatatt aagtaagtat aattatgata aatctattaa
    37021 gataaactct gagcctatta tttctaaatt agttttagag actatcaaaa taaatataat
    37081 attagtatgt ataatattta ttttgatatt tgtgttggtt gttagatatt ttacgtcaat
    37141 ttacaatgat ttatcagata tgactaagta tgtttactat agttcagagg gtaagccatt
    37201 tgatataaaa aacaagaatc aagaagggca gataggtctt ctaaaaactg aactcttaaa
    37261 gatgacgaca attcttaatg aaaaggttga acttttaaaa agagaaaaga tatttttaaa
    37321 caatacaatt tccgatatat cacatcaatt aaaaactcct atgacttctt taataatgtt
    37381 gaatgattta ttatataatg atgtaccata cgaagtaaaa atcgattttt taaataagat
    37441 aaaaaatcaa ttaaatagaa tggattggtt gattaaaagc atgcttaaat tatcaaaggt
    37501 agaagcaaaa gtgataaatt ttaaaaaaga taaggtaaaa tttagtgaac tcatacatag
    37561 agcaatgcta tctatgaaaa tacctatgga gattaaaaat caaaaactga ctatagaggg
    37621 aaatgataat gtatcttata ttggtgatat tgattggtcc gtggaagcat tagtgaatat
    37681 aatcaaaaat tgtgtagaac atactcctga atttggaaat ataattataa cctataaaga
    37741 aaatccatta ttttctgaac taataataaa agatgagggt gaaggtatag ataaaaaaga
    37801 tataccacat gtatttaaac gattctatag aggaagaagt agttcaaaag aagatagtgt
    37861 aggaataggt cttgcaatgt caaaatctat aatagaaagc caaaatggcg atatatatgt
    37921 aaacagcgaa aaaggtaagg gtacagaatt tcatataata tttcataaaa tgtatgatag
    37981 tgactaatct gtaatattta attcacttta tagtaatagc caaaacctaa tatatgaagt
    38041 atagaatata aatattggag gtagttatgg aaattttaag agtagaaaat ctaactaaaa
    38101 gttatggtaa aaatgaaata aaagttgatg ctataaaaaa tgtaagttta tcagttgaaa
    38161 aaggaacgtt tatagcaata acaggtccaa gtggaagtgg taaaagtacc ttattacatt
    38221 tattaggagg agttgacaga ccaactagtg gtaaggttta tataaatgat gttgatatat
    38281 ataatttaaa aactaaggat ttggcgatat ttagaagaag aaatataggt cttatatatc
    38341 aattttacaa cttaattcca gtcttaaatg tgaaggaaaa catattgctt ccagcagagc
    38401 ttgacaatag agatattgat agagaatatt ttgatgattt aatgaaaaca ttaggtctaa
    38461 ttgatagaga aaagcacctt ccaaaccaat tatcaggagg tcaacaacag agaacctcta
    38521 taggacgtgc attaattaat agaccttcta taatattagc agatgagcca acaggtaatt
    38581 tagatagtaa aaattcaaag gaaatattag aattgttaaa attgtcagta aaaaaataca
    38641 atcagacact aataatgata actcatgaca aaaatatggc acttcaagct gatagaatta
    38701 taagcataga agatggaatc ataaaaagtg atgaggtgat gtagatgaat ttatatacat
    38761 cactaaccat tagatactta aaacaaaata aaagaagaac cattgtaacc ataatcggta
    38821 taatactttc aacagcttta atttgtggta taggaaatat atttgaaagt tttatggatt
    38881 atcaaatgag agaaactata aagaatgatg gaagtttcca tgttacattt tatgatgtaa
    38941 atagaaaaaa tgcggaatat gtaacgaaat cagctgaaat agaaaaacat gcatttacca
    39001 aacaacttgg atattctaaa ttagaaaata gtgaaaatgc tatattaagt ataaagcaat
    39061 atgataaaaa tgcaataaat ggatataaag tttctataaa agaaggtaga tttccagcta
    39121 aggaaggaga aattgtttta agtgaaagta ttattaatct tatagatgat aagttaaaaa
    39181 taggagataa gataacttta aaagtagggg atatgtttga ctcaagcaag aaaaagattg
    39241 atagtatgac atttcatgaa ggtgattata tagcaaatga aaaagataga acttttaaag
    39301 ttgttggtat aattgaaaaa cctgggtttg aacgttataa tggaattgca acagctatag
    39361 cctactttaa tcctatgaat aattacaatg atacaattaa tgtatcagta gcagtaagaa
    39421 atcctaaaga tgtgtataag ataagtaata aaataagtaa aaatatatat tcaaataaag
    39481 ataaagaagc agtttctgat atgataaaat acaatgaaca tttattgagg ttacaaggtg
    39541 ctagtaagta ttcaaatata aatagctcta taaagtctat aattatagca gtaactattt
    39601 tagtaatcat atgtacaatt gctacagtgt acaattcctt tagtatttct ataaatgaac
    39661 gaaaaaaaca gtttgggata ttaaattcta taggtgctac atctagtcaa attaaaagat
    39721 tagtttttat agaaggtatt ataattagtt taattggtat accaatagga ctaatatctg
    39781 gaacagtagc tatagactta ttgtttaaaa ttataaataa gtattttact gaatcggttg
    39841 ttactaagat gagtttgcag atagtgtata atccaataat aattattgtt agtataataa
    39901 tagttttgtt tacaatcttt atatcaattc tactacctgc aatatcggct tctaatattt
    39961 ctccccttaa tgtgattaaa aatactggag agtataaagt gggcaaaatc aaaagctcaa
    40021 gacttattaa aatgattttt aaaactgaag gagtacttgc atataaaaat attaggagaa
    40081 atagaaaaaa atttataata actctttttt ctttaatggt aagtataata atatttatat
    40141 cttttagtgg ctttacatta cttttactaa gaggtgaaga aattagaaat tctcaaagaa
    40201 aatatgattt gtatttaact gcaaagaaca cagctaggtc tgtagaggat actataagtg
    40261 aactagaaga tattgatggg ataaaaaact ttgaattggc aacaggacaa tatgtgtcta
    40321 taagagtaag tgaaaataaa ataaataaat ccaaggaaga tttaatcagg aaatattatc
    40381 aaaaatataa gattggagat agttatgaat atgatttcag taataatgaa ttaatttttc
    40441 caggagattt tgcagtaaaa aacaaaataa ataacttagt acagggctct tttaataaag
    40501 agagagcaat tgaagaaaat ggtgttatat tagttagaaa aagtgcattt gaagaaaatg
    40561 ggaagaaagg tgtagtagaa cttaccaact ataaagtagg agatacagta aattgtgaat
    40621 atcttgatga aaatgcctta agtaaaaaag taaaggtcaa gattcttgct ataactgatg
    40681 aagaacgtct aggtcttggt tatcaaaata tggggttaca atttataact tatgatgaag
    40741 ttgctaaaaa tttaaattta aaattaagta aaagtcttat atttattgat tcaggaggaa
    40801 gcattaagac taagaaggaa atagaagctt tggcaaataa aaataatttt aacttttatg
    40861 atgaaagtgc aagcaatgaa agtgaaaaac aagatttaaa ggttattaaa atatttgtat
    40921 atggttttat agtagtgata tctttagtga gtgttacaaa tattctaaat acagtaagta
    40981 caagtataaa tctaagaaaa agagaacttg cagtaataca atctataggg gttactccta
    41041 aaggatttag gaaaatgata tatttagaaa gttttatata tggtatactg tcattgttgt
    41101 ttggaatacc aattagtatt ggtataattc taattatgaa taaacttatt tcaggtgtaa
    41161 ttgaattttc tccgattata ccgtggacag ccatagttat ctgtatagta agtgttttta
    41221 taataacttt tatagcaggg tatataccaa tgagtaaatt aaataaagaa aatataatag
    41281 ataatataag aagagaaagt atatagatat aaaaataatt cttaactata taaaatatta
    41341 ttaaaatcat ttaagtggta taaaatatta ttatatcact taaatgattt tttatttata
    41401 taagaaatct aataataaat tactagaggt atttaactat ttagaaagaa ttggtattat
    41461 ataaagtaag agaatatata ataagaaaga atatttgatt ttattggaaa acaaaagaaa
    41521 aagaaggtga ttactatttc tcatataact gcgaagaata tgtataaaag tttagaggaa
    41581 agaataaaca agtttccaca aggtgctcca ccatctgata cattatacaa aatactaaat
    41641 gtactatata cagaacaaga ggcgaagtta gttgctcagc ttccaataaa gcctttcaga
    41701 gtaaaaacag cagctaaaat ttggagtgta agtgaaagtg aagcatatag agtattggat
    41761 aaacttgcta gtaaggctct gatactagat atagaagata ataaagggaa gaaatatata
    41821 atgcctcctc caatggctgg tttttttgag tttgccatga tgagaacgag acacgatata
    41881 gaccaaaaac ttttggcaga gctttactat caatatatga atgtagaaga agattttatc
    41941 aaagatttat tttattcaac agaaacgaag cttggaagag tatacgttca agaggaagtt
    42001 ttaactaatg ataatgaggt tagtatttta gactatgaaa gggctactca tataattgat
    42061 gaatcaactc atataggtat aagtatgtgt tactgtagac atagaatgca acatgttgga
    42121 aaagcttgtg atgctcccat ggatatatgt atgacgtttg acaatgtagc aaattcttta
    42181 ataaataata aatttgcaag acgtgtggat aaaatagaat gtaaagaact acttcatcaa
    42241 gcatatgaac ataacttagt tcaatgtgga gaaaatgtta ggaaaggggt tacgtttata
    42301 tgtaattgct gtggatgttg ctgtgaagca atggtagcag ctaaaagatt tggaaatctt
    42361 catccagtac agacaacaag ttttatacca aatataaacc atgaaaattg tgtaaagtgt
    42421 ggtaaatgta taactgcatg tccaatagat gcgataagca aggttaagga agatggtaaa
    42481 gaatatataa aaattgatga ggatagatgt ctaggttgtg gagtttgtgt tagaaattgc
    42541 cataaaaata gcattatgtt attaaagaga gatgaaaaaa taataactcc agcaaattca
    42601 gtacatagag cagttctcat ggcaattgaa aaagggcagt tacaaaattt aatttttgat
    42661 aataatgctc tggctagtca tagagctatg ggagctatac tgtcagctat attaaagctt
    42721 gaaccagcta aaaagatttt agccagtaaa caattaaagt cagtatattt agataaattg
    42781 ttaagtatga atgataaata gaaactaact taaaaaaggc aaagttttag acttatctat
    42841 atcttaataa acttatcatt taatatttaa atttaatttt tacacatata aaacttataa
    42901 cttaaataaa aaaaggctat attcaattaa tattacttga atacagcctt ttttagttat
    42961 gttaagctat ttcttttatt tgcttaaatg agttttcatt tccatttcca ccaatttgtg
    43021 ttacagattt aaatctcata gttttaaata gttctttttg attataatct agtgagtttc
    43081 ctactagcat tacaggagat ttagtttttc cagccaaaac tccaacagat aatccgtcta
    43141 ttaaatcatc ttgtttattc attccatttt tagcaacata taaattgtct atcttagaat
    43201 ccttgtaaaa ttcttttatt acttttgcat tagtttcatt tctagtgcta cctgatattc
    43261 tctgtggatt ttgaagttta ctttctatat ttttagaaac tgtatattct ccaccgatta
    43321 cataagattt atcaatcttt ttgtttttaa ataagttatt tatttcagtt atattactat
    43381 tttcattagt aagtattata ggcatatcat tttgagctga tacagctcca atgcttactg
    43441 catcggctaa tcctttttcg ccattaacta ctgaaatctt tgatatattg ctaatattgt
    43501 tcaattcttt agcaatgttc atagaagttt gatatctatc acttcctgaa attctagata
    43561 cagagatatt gtttgatttt atagtatcta aagatttttc atttatagaa gcttctccac
    43621 caactacata tacattttta acttttaatc tctttaattc agccaaagtt ttttcattta
    43681 tttgagagct tccagtcaat aatataggag cgtttttctt gtatgcaaat ggtgttgcag
    43741 ccaaagcatc tgcaattgct gaatcgttta ttaaaactgc attttcagca ttttcccaac
    43801 ctgcttgact tatcttaata gaagtttcat atctatcagt tcctgtaagt gcacttgcat
    43861 ttacttcatc ttgatatgta acctcaaatc tagcatttgg acttcctaag aaaacaatta
    43921 cgcctccttt tggcaatcta actctattgt tctttgtaac taaagaatag tttacaggaa
    43981 cacctttatc atcataaaca gcaaaagctg aattttgagg aagattaact tctatttttt
    44041 tatttgcgat atcgtcacca atcttatacc attttgcata accattggat tctagttcac
    44101 aagtaaaaga tttttctgct ggcaaattag ttatagaatc ttcactaaca tacgttagtg
    44161 ttccaaaaga taaatactct gtaccatttt ctttatgaag tttaatgtcg gataaatcac
    44221 gacctattac accaggtatt tctataaaag catttgagtt attctcatcc attattttag
    44281 tgttaactat ataacctgga gtttcatcag aaagactaaa acttgctttt acagagccaa
    44341 acatatagct ttgagaagta tatttttcat ccactagata gtaacctttg ccacttcttt
    44401 ttttccacac ttctttgaca ctatttgata tattgttgtc atctactttt tgagctacat
    44461 agtataaact tgctgtctga cctaatccag ggacatcatc ataagagctc atatttaaat
    44521 atgtgatatt atttttttct tttacaaatt ttaaacaact attacctttt tcagatacaa
    44581 atctatcttg acctatgtat acgtatttat cttcaggtcc attttctata taaggagaag
    44641 atactgttaa agtaccatta tcattaacat ctacttttat catatttgaa gaagcataaa
    44701 gaccactatt ttcttttaaa gagctaggca ttttaacttg ttgtggctta ctaaatgttt
    44761 tatcaggttt tatttcttta attttacctt tttcttttaa tgcagatagt aagatttctt
    44821 gtccaattat ttcattaagt tgactacttc caccagaaga aagaactgca actgccatat
    44881 tttcatctgg aagaactata agattactgt ggaataataa agagtctcca ccttttgtaa
    44941 gagcttttaa attatactga ttaaatggat aagtgttgac acaatcccac cctaaaccat
    45001 atccaagtat agagtcttct ccttcaggcc atagaccatt taaatattct ttgttttcca
    45061 tagcttttac tgaagcagga gaaagaatac catttgaatt tttcataaat gtttgtgcaa
    45121 atgtacatag attttctgca cttgaatata aaccacctgc acctatagca ttaaggttat
    45181 cttgtggaac tgcatcttcc caataaggta cgtatgcttt tgctagtttt gaactatcaa
    45241 agctattttc tgtagttttt gtattttgta agttaagtgg attatttatg tatttatcaa
    45301 gaaaattagt aaatgacata ccagatactc tttctactaa aatttcagct aaagtaaatc
    45361 catcattaca ataaacagaa aaagctcctg gtttagcttt taatctttgt ttttgtaatt
    45421 cttttaagaa attatcatga ccataagaat cattgtctgc taataaaata gtgtttttaa
    45481 aactacttcc catcaaccct gatgaatgat ttaataacat tcttggagtg atttccttgt
    45541 atctatcatc agccatttta aattcaggta tatactttac aacaggagta tctaaattta
    45601 attttccatc atcaaccaac ttcataacag ctgttgtagt aaacatttta ctgatagatg
    45661 cgatactata catattatcc ttatttaagt ttttgttatc ctgtttgctg taaactccac
    45721 cattaccaga aatctcaatt ttgccattat caatcaaagc atattgagca cttacagcac
    45781 catactttga tagcaaagtt tggattttct ctttagcaag agctttagtt tttgtgtaag
    45841 attccgcgtt tttactataa tcatgtaatt ctatatcaga actttgatat ttttcggaat
    45901 tatcttttgt attatcagca aaagtaatat ttggagcagc agtaaataac atactcaata
    45961 ctaatgtact agataataat ttagagagcc tttgcttaaa catattacat tacctctttc
    46021 cctttttaat aatttataat tagcataaca ttcttaatta ttttaatcaa cagattatta
    46081 tcaataaaaa aagcttttat aagtatagct ttttatataa cagagaaaaa tataaaaata
    46141 atatttgttg ttgtataaag aataaagggt ataggtaata tataaataaa atggaggaat
    46201 tttagacaat gtataagaat ataaataaga agcaacttaa agaaatgatg aaaaatgaaa
    46261 aggatgtttt attattagat gtaagaacta aagatgaatt taaggaatat aaaatagaaa
    46321 atgctataaa tatatcttta caagagttaa tcaataatat agatgaaatt tacgattata
    46381 aagataagaa agtagtagtt tattgcagaa gtggtcatag aagtgttact gcatgtaatt
    46441 tgttagctga agaaggtttt gagcatttat ataatttaaa ttcaggaata attgattata
    46501 tgagttaaat aatgtaagtt atacattaaa tactatgtat agctctgctt attaagtagg
    46561 aatatatcta aatatttata agataaattt aggaaagaac gaattttttc gtttttttct
    46621 atgacagttt ttgtagtaaa tattagtttt taatttggaa aaaggtagta gctttttagc
    46681 tactaccttt taatatgaac ttagaatatt attaaaaaaa tatatttaaa gacagaatta
    46741 attttttaat gaccagttaa attactatcg ccgaatttat cattaaatgg ttttataact
    46801 ctatttatag gtccttttag aagtacattt aaacaaatag acagaatcat aaatagtaat
    46861 agaactagta tatctttagt tagatttgga acatatatac caccaactgc ttcccttgta
    46921 gcacctattg cataggtaaa tggcattata gggtttattc tttggaagaa tgtaggtgta
    46981 acttgtatag ggaatgtacc accagaagaa ccaatctgaa cgactaatag tactattgca
    47041 attgcttttc ctacatttcc aaatacagaa actaaagaat acactataaa gttaaatacc
    47101 atactagtaa acataagcag aactatcaat aaaattggat gttttgctgt tactcctaac
    47161 aataatatat ctccagttcc tattataaaa ccttggacaa gagctatact taaaaaagta
    47221 aggcctcttc caaagtaaat ttcataaggt ttatagtttc catgtacaga tgtagacaat
    47281 agtgctgaaa taagtaatat accaacccat gtagaaagaa cactataaaa tggtgtcatt
    47341 gcagaaccat aattttcaat tggatataat ttctctgttt tcacctcaac aggttctttt
    47401 aagaaattag actttgtcaa tatatcactt tcaagcaagc tgactaattt tttcatatct
    47461 tcaccattac taattttgct taaagtatca atcaaatcat ccaacattcc ttttgctaaa
    47521 ggaagtcttt ctcttatcaa tgatatactt tcctgtgcat ttcctgaaag ttttagagaa
    47581 gtagtaagta tttcttcaac ctttggaagt tttgcttctg ccttatctaa aacagtaatt
    47641 atatcatttg ctacttttat actgtttgca aatatactat ttataggttt tgatattttt
    47701 gagtcaaaat tatttaaaat atttaaatta attcttccaa taccatttga tagacttaaa
    47761 acattattca aagcacttat agatggttgt tgtccagata taactttgtt tttaatatca
    47821 tttaatgttg atatagatga atctatttta ttgctagagt cttcaagatt atctatgaca
    47881 tcatccaatc tattatttga tgtaagttgg tttaatttag ttaaaaaatc caccaaagta
    47941 tcatttaaag attgcaaatt agaaagtttt tctgataaat tatctatcaa cttaggtaca
    48001 tcttcactgc cactatttac tgcatcaatt aaatttaatg taagagaaga agcagaagat
    48061 gaaaggtcaa ccattaaatt taagtcagac ttaataagag gagaaagttc gtctaaatta
    48121 tcatttgtat cctctaagaa tttttttaag tcacttgata acagttttgt atcattcaga
    48181 gtctttttaa ttgtaggtaa atcagattta atatctttaa ctgtatcaga taattttgaa
    48241 gtagcatcag aagctaaatc aacagtctta tttatatggt caaatctctt ttgaaggtca
    48301 ataagggaat tttcaacttt tgtcaatttt ggaagactgt gttcaattcc tgcaccaact
    48361 ccgtttgata taccaagaac gatttcacta actgttttta ctatagtctg attaacctgt
    48421 agttgtattg tagaagcacc tttatctgta atctttggtg caactgcatt tattttttca
    48481 ttaacagtat agataatttc ccctttttta acatcatctg taattaaaga tgtcaaatct
    48541 tttgtaaaat tttttggaat cttaagtgat gcataataag tacctttttt aacaccttca
    48601 agagcagttt tttcatctac aaactgccaa cctaaactct tgttatcttt gagttgttca
    48661 attaattggt ctcctacatt aatcttttgt cctgctattt catttccttt atcttcattt
    48721 gttactgcta caagcatatt agcagtgctt ccataagggt cccaagatgc ctttatattg
    48781 aaccaagcat acagagatgg taaaaaagca agacctaaaa ctattacaag tacggcataa
    48841 ttgccacgta tatcttttag gtcactttta aaaatttcta atatattttt taaacccttt
    48901 gaaggtctta aattcttgat attattccag tttaaaattt cttttaaact attagttttt
    48961 ttcaaatgtc tcacctccta aaatattttg ataaagaaaa aagttagatt tataatcaat
    49021 tttaaattat attacttttt acacaaatag taaagttaaa attttgtctt aattttaata
    49081 catatatatt atatataata gtatgctata aatatatata aatataggaa ataaaaacaa
    49141 ataatttata aaatatatgg tataaaaatt aataataaat caaatattta taattgatat
    49201 aatcaattaa attagccatt atgatatact tttggtaata agaaaattct atgaagggat
    49261 gattgaatga ggtattttaa aaaatacata aaaaaatata tagtattatt tctaacagca
    49321 gtattttttt taacattgga agctttttgt gatttagctc aaccaactat aatggcaaaa
    49381 ataatagata taggggtttc aaataagaac ttagactaca tactatcaac aggagctata
    49441 atgataggta taactttttt aggtgcaata tttgcaataa taagaagtgt aatctcggca
    49501 agagtttctc agcatttttc ttatgattta agacaggatg tatttgaaaa gataaataca
    49561 tactcattaa aacaaataga taaatttggt agagcttcat taataacaag aattactaat
    49621 gatatcaatc aaatgcaaca attcgttaat ggaatgatga gagtttttat aaagtcacca
    49681 ataatgtgta taggaagttt aattatagct ataaaattaa atttaaagat gtcaataatt
    49741 attctaattg cagttttaat tatatttatt ataatattgc ttaatatgaa gatagggtat
    49801 agattattta aaaatgtaca agaaagtaca gataaagtaa attcaaaatt aagacagttt
    49861 ttaagtggaa taaaagtagt taaactattt tgtagatacg attatgagaa ccaaagcttt
    49921 gaagagttaa atgatgaact ttttaaaaat agtagaaaag ctacaactat gatgtcattt
    49981 tttagaccga caattacaat aatcattaat gtttccataa taataatatt actgattgga
    50041 aggcatttaa tttattcagg agaaattaaa attgggatta tagttgctta tgtaaactat
    50101 atgactagaa tattaacatc tcttataatg atttcacatg tttttaatgt ttttgttagg
    50161 gcaaaagcat cttatgagag aataagtgag gttttggttg atgagaatga agaagtaatt
    50221 gagagagaag aaagtttaga cgttggagaa aaagatttaa agtttgtaaa ctgtgatata
    50281 atttttgaaa atgtagattt ttcgtataac aaaaatacag atgaaaaaat attaaaaaac
    50341 gtatccttta aaatagaaga aggtcaaaat attggtgtga ttggtcaaac tggttcagga
    50401 aagacttctt tagtaaattt aattcaaaaa ttatatataa ttgataaagg tgatattaag
    50461 attggaggac gtagtatata cagtatttct gaagaaagtt taagggaaag aataggtgta
    50521 gtatttcaaa atagtagtgt gtttggaaaa agtatattgg agaatgtagt tatgggaaat
    50581 agagatatat gtaaagagga tgttgtaaaa tcatgtgaaa tagcaaatgc aaatcaattt
    50641 ataacaaaac tcgaaaatac ttatgatgaa gttttattcc aaaggggtaa aaatttatca
    50701 ggaggacaga gacaaagaat tactatagct agagctatag ccaaaaaacc acaaatatta
    50761 attcttgatg attgttttag tgcaatggat attaatacag agacaattat aagaaagaat
    50821 ataaaagagt atataagtaa tttaagagaa aaggaaagta agaaatgtac tgtaataact
    50881 atttcacaaa agattagttc tataattgac tgtgataaga ttttagtctt ggatgagggt
    50941 gaggtggttg gatttgctac acatgaagat ttactggtta gttctccaat ttataaaaaa
    51001 atttttgcgt tgcagaattt agtaagtgaa gatgtaaatt aaggaggaag tatattgaat
    51061 ttaagtactg gaaatcagaa ggtcagtaga ttaaagaaaa aagaaaagcc taaagatttt
    51121 aaaaaaacgt ttaaaagaat ttttatgtac tttaaaaaag ataagaagat tactagattt
    51181 atttttttgc tagta
//
LOCUS       DAEMHZ010000010        47608 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000010 DAEMHZ010000000
VERSION     DAEMHZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.010"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            207..518
                     /locus_tag="KPK72_000534"
     CDS             207..518
                     /locus_tag="KPK72_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2487779.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            566..883
                     /locus_tag="KPK72_000535"
     CDS             566..883
                     /locus_tag="KPK72_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2487780.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            1096..1797
                     /locus_tag="KPK72_000536"
     CDS             1096..1797
                     /locus_tag="KPK72_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487781.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            2021..3322
                     /locus_tag="KPK72_000537"
     CDS             2021..3322
                     /locus_tag="KPK72_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2487782.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLVLVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            3459..4553
                     /locus_tag="KPK72_000538"
     CDS             3459..4553
                     /locus_tag="KPK72_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2487783.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFLKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            4555..5688
                     /locus_tag="KPK72_000539"
     CDS             4555..5688
                     /locus_tag="KPK72_000539"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG2487784.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            5706..6815
                     /locus_tag="KPK72_000540"
     CDS             5706..6815
                     /locus_tag="KPK72_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG2487785.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVESGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRSMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            6880..7620
                     /locus_tag="KPK72_000541"
     CDS             6880..7620
                     /locus_tag="KPK72_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG2487786.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            7625..8617
                     /locus_tag="KPK72_000542"
     CDS             7625..8617
                     /locus_tag="KPK72_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG2487787.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(8805..9686)
                     /locus_tag="KPK72_000543"
     CDS             complement(8805..9686)
                     /locus_tag="KPK72_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2487788.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            9959..11347
                     /locus_tag="KPK72_000544"
     CDS             9959..11347
                     /locus_tag="KPK72_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487789.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNIAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            11375..12328
                     /locus_tag="KPK72_000545"
     CDS             11375..12328
                     /locus_tag="KPK72_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2487790.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            12388..12612
                     /locus_tag="KPK72_000546"
     CDS             12388..12612
                     /locus_tag="KPK72_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487791.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQDREATNYNIGNG"
     gene            12605..13117
                     /locus_tag="KPK72_000547"
                     /pseudo
     CDS             12605..13117
                     /locus_tag="KPK72_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            13149..14003
                     /locus_tag="KPK72_000548"
     CDS             13149..14003
                     /locus_tag="KPK72_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG2487792.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            14537..15331
                     /locus_tag="KPK72_000549"
     CDS             14537..15331
                     /locus_tag="KPK72_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2487793.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            15435..16241
                     /locus_tag="KPK72_000550"
     CDS             15435..16241
                     /locus_tag="KPK72_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2487794.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTASIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRYSPLKA
                     SIIVGFCWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            16303..16824
                     /locus_tag="KPK72_000551"
     CDS             16303..16824
                     /locus_tag="KPK72_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2487795.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCILLEETLRKMK"
     gene            17234..19321
                     /locus_tag="KPK72_000552"
     CDS             17234..19321
                     /locus_tag="KPK72_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2487796.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            19336..19623
                     /locus_tag="KPK72_000553"
     CDS             19336..19623
                     /locus_tag="KPK72_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2487797.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTELEKFLASKEE"
     gene            19650..20921
                     /locus_tag="KPK72_000554"
     CDS             19650..20921
                     /locus_tag="KPK72_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG2487798.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTNWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            20979..21578
                     /locus_tag="KPK72_000555"
     CDS             20979..21578
                     /locus_tag="KPK72_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2487799.1"
                     /translation="MENTLSEFLDNISNELSVFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            21583..22488
                     /gene="dapA"
                     /locus_tag="KPK72_000556"
     CDS             21583..22488
                     /gene="dapA"
                     /locus_tag="KPK72_000556"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2487800.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            22594..23226
                     /locus_tag="KPK72_000557"
     CDS             22594..23226
                     /locus_tag="KPK72_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG2487801.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVRKI"
     gene            23704..26211
                     /locus_tag="KPK72_000558"
     CDS             23704..26211
                     /locus_tag="KPK72_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2487802.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTDMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQEYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            26553..28715
                     /locus_tag="KPK72_000559"
     CDS             26553..28715
                     /locus_tag="KPK72_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG2487803.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            28720..29337
                     /locus_tag="KPK72_000560"
     CDS             28720..29337
                     /locus_tag="KPK72_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487804.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            29526..29870
                     /locus_tag="KPK72_000561"
     CDS             29526..29870
                     /locus_tag="KPK72_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG2487805.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            30440..31306
                     /locus_tag="KPK72_000562"
     CDS             30440..31306
                     /locus_tag="KPK72_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2487806.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            31419..31823
                     /locus_tag="KPK72_000563"
     CDS             31419..31823
                     /locus_tag="KPK72_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG2487807.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            31990..32439
                     /locus_tag="KPK72_000564"
     CDS             31990..32439
                     /locus_tag="KPK72_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2487808.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(32595..32972)
                     /locus_tag="KPK72_000565"
     CDS             complement(32595..32972)
                     /locus_tag="KPK72_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2487809.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            33234..34151
                     /locus_tag="KPK72_000566"
     CDS             33234..34151
                     /locus_tag="KPK72_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG2487810.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            34635..36053
                     /locus_tag="KPK72_000567"
     CDS             34635..36053
                     /locus_tag="KPK72_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG2487811.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            36129..36941
                     /locus_tag="KPK72_000568"
     CDS             36129..36941
                     /locus_tag="KPK72_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2487812.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            37377..37994
                     /locus_tag="KPK72_000569"
     CDS             37377..37994
                     /locus_tag="KPK72_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG2487813.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(38151..38603)
                     /locus_tag="KPK72_000570"
     CDS             complement(38151..38603)
                     /locus_tag="KPK72_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG2487814.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            39051..40409
                     /locus_tag="KPK72_000571"
     CDS             39051..40409
                     /locus_tag="KPK72_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2487815.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            40682..41062
                     /locus_tag="KPK72_000572"
     CDS             40682..41062
                     /locus_tag="KPK72_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2487816.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            41187..41387
                     /locus_tag="KPK72_000573"
     CDS             41187..41387
                     /locus_tag="KPK72_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487817.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            41583..42311
                     /locus_tag="KPK72_000574"
     CDS             41583..42311
                     /locus_tag="KPK72_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2487818.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            42333..43250
                     /locus_tag="KPK72_000575"
     CDS             42333..43250
                     /locus_tag="KPK72_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG2487819.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            43392..44576
                     /locus_tag="KPK72_000576"
     CDS             43392..44576
                     /locus_tag="KPK72_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2487820.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            44683..44871
                     /locus_tag="KPK72_000577"
     CDS             44683..44871
                     /locus_tag="KPK72_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2487821.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            45020..45670
                     /locus_tag="KPK72_000578"
     CDS             45020..45670
                     /locus_tag="KPK72_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2487822.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            45773..46711
                     /locus_tag="KPK72_000579"
     CDS             45773..46711
                     /locus_tag="KPK72_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2487823.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            46737..47588
                     /locus_tag="KPK72_000580"
     CDS             46737..47588
                     /locus_tag="KPK72_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2487824.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT        18745 a         4962 c         8396 g        15505 t
ORIGIN      
        1 atatctaatt aagataagta aaaaaagctg tcacagaaca aatttaattt gctaatgcga
       61 cagcccattt ttgttaattt gacacttaat aatattaaaa taacaaaaaa gtattgaaag
      121 tatattgcat ttaaggtaga attaaataaa atattataac attataatgt ttatatagtt
      181 gttaataaaa atttggagga aaatatatga taaaaataat gttggcctgt tcagcaggaa
      241 tgtctacaag tttattagtt actaaaatgg aaagtgcagc taaagaaaat ggtatagagt
      301 cgcaaatttg ggcaatacca gaatcaacta tacaaaatga aatagaaaaa tgtgatgtat
      361 tattactagg accacaagtt agatatgtat tacctaaggc tcaagaaatt gctaagccat
      421 ataatatacc agtagaagtt ataaatatga tgcactatgg aacagttaat ggagaagctg
      481 ttttaaatag agcgatagaa cttaacaaca ctaagtaata taaattataa tgtattttta
      541 atatataaat tgggagaatc aaaggatgga tgaaaagatt atagaaatat cttttaatat
      601 aataggttat gcaggtgaag gtaaagggtt agcttttgaa gcaataaaag aggctaagaa
      661 tggaaatata gaaaaagcaa aagaattatt aaaagagtct agggaagtta taaataaagc
      721 acatagatat caaacagaat taatacaaaa cgaagctagt ggaaacagga ctgaaataag
      781 tgtcatactt atacatgcac aggaccactt aatgaatggc atgaattttc aacagttagc
      841 tgaggaaatc gttgatttac atttgaaatt acaagataaa taaaaattaa agataataaa
      901 ttaagagagt tcttaaaaga tacattatgt gtagcacact aattgttaga ttattgattc
      961 aatatttagt gagtaataca ttaggattat tttgaggact ctttttatat ttagtttaaa
     1021 gttaataaat atttattatt aataatataa gattatatgt tataatatta taacatataa
     1081 tttaagaggt gaatcatgtt aagaaatcaa ccattatata tacaagttgt agatatatta
     1141 aagcaaaaaa tattaaataa cgaatataaa attgatgaaa atattccaac agaaagggag
     1201 ttagagcaag agtttggagt cagtaaaatt actattagaa aagctattga gatattagaa
     1261 tcagaaggat atgtagagaa aaaaagtgga aaagggacaa aagtaataag taatagcctt
     1321 tttaataagc tatcaaaagc agagtctttt tcaagtatac ttttatctaa aggtcataag
     1381 ttatctaaag agactatttc aattgaaaaa ataataaatg atacaaattc acaagtattt
     1441 agtatatttg gagaaaaatg tagcaagatt actagaatgt attatttaga tagtaagcca
     1501 tatatatttt ttactcatta tattcctttt gatgaaaatc taactgttga aagttttaat
     1561 aatgaaagtt ctttatatat gtacttatat aggagaaata taaatataac tagatttgaa
     1621 gatgaatttt ttatagaaga atcagaacta gaggtatcaa aggctctaaa tctagctaaa
     1681 gacatattat taggaagaaa aagaaagacc tatgatgaga atgataatat agttgagcta
     1741 tcatttgcaa gatataatac agaagtacaa aactatgtaa ttaaatacga aatataataa
     1801 gaatcctgag tttattgaaa tgataatatg ctcaggattt ttgtttagtt gtaaaaacaa
     1861 aaaatattat aacattataa tgtttacaac taaacattat aatgttataa tatttttaat
     1921 aaataagtga tattatcaat tacataaaaa ttagtttatg ttatgaaatc gttattgcat
     1981 tataaataaa ggataatatc aataatacag ggggataact atggaaaaat tcgcaagttt
     2041 tttagagcgc aagattatgc ctgtagcagc gaagataagt aatcagagac atatgagagc
     2101 tgtaagagca ggtataatag caacattacc actaacaata gtggggtcat ttttcacaat
     2161 attattaaac atacctattg atgcttatat ggattttata gctccattta aagcaatatt
     2221 agatatacca tttagatata ctgtaggatt tttatctcta tatgctactt ttgggatagc
     2281 atcatcactt gctaaaagct atgatttaga tacaacaggt gttggtatgc ttgcagttat
     2341 ggcattttta gtttctacaa tagttccaac tcaagtagtt gaaccagtag gtggagttat
     2401 agaagctggt agatggatgg atatggcaaa acttaattcg ccatcattat ttggagctat
     2461 cgtaacatca ttaattacag ttgaaatata tcgttttatg aaggaaaaga atattacaat
     2521 taagatgcca gcaggagttc cagtagaggt ttcaaattca tttactgcat tattaccgac
     2581 agcagtaatt ttaatgttat tctgggtaat aagacatatt ctaggatttg atataagtgt
     2641 tgcactaagc gctatgttat cacctttaaa aggattctta tcaggaaata gtttaggtgg
     2701 tgggttactg acagtattcc taataacttt attctgggta cttggaatac atggtccagc
     2761 tataatgggg ccagtaataa gacctatatg ggacatgtct atagcagaaa atataactgc
     2821 attcacagaa ggagtatcag ctcatgcaat gccaaacata tttacagaac aatttttaca
     2881 gtggtatgta tggatagggg gagcaggggc tactcttgta ttagttgtaa tgtttatgtt
     2941 ttctaagtca caatatctaa aaagtttagg tagattagga tttttaccag ggttgttcaa
     3001 cataaatgaa cctataatat ttggagcacc aatagttatg aatccaatac tagggatacc
     3061 atttataatt ggacctataa taatgacaat tctatcatat gttcttacta ttacaggggt
     3121 tatacctatg atggtagcaa gattaccatt tgcaatgcca gcaccaatag cagcagtaat
     3181 gagtactaac tggagtatat tagcaggtat cttagtatta atcaactttg tgatatcatt
     3241 tgctgtttat tatccattct tcaaagtttt tgaaaaacaa caattacaaa gagaacaaga
     3301 agaaatgagt gaaaatttat aaaaatacat agatatagag gttttgtact atataaaata
     3361 tagaaaaaac ttataagtta ttaagggtga aggtaggagg aatgatcttt gcctttaatt
     3421 ttatatattt taaataatgt aatttgagga ggagaaagat ggaaagaaga ttaggaatat
     3481 cagtatatcc agagcatagt aacatggaag atgataaggc atatataaaa atggcttcaa
     3541 aatacggatt taaaagaata tttatgtgca tgctttcaat tacaagacct aaggaagaag
     3601 ttaaaaaaca ttttaaagaa attataacct atgcaagaga attaggattt gaagtaatcc
     3661 ttgatatagc tccaaacata tttgatatat tacaaatatc ttatgatgat ttaacatttt
     3721 ttagtgagtt aaatgctagt ggaataaggc ttgactatgg atatgatgga agtaaagaag
     3781 ctatgatttc tttcaatcca tatgatttaa aagtggaact aaatatgagt aatgatgtag
     3841 catacttgga taatataatt acttacaagc caaatgtaaa taatatatat ggttgtcaca
     3901 atttttatcc tcaaaggggt acaggcttgc catatgattt ttttgtaaga tgttcaaata
     3961 gatttaaaaa gtatggaata agaactgctg cttttgttaa ttcaaaatat gcaacaatcg
     4021 gaccttggaa tataaatgat ggattgtgta cgcttgaaat gcatagaaac ttagatattg
     4081 ctacagcaac taagcactta ttcgcaacag gattgattga tgatgtaatt gtagggaatg
     4141 catatgcaag tgaagatgaa ttaaaaagaa tgtctgaagt aaataaatat caagttgtat
     4201 ttaatgtagt aactgaggaa aaaacttctg aagtagaaaa gacaataatg gcaaatgagc
     4261 aacactttag aagaggggat ataaccaacc aagtagcaag gtctacagaa gtaagaaaaa
     4321 aatatgctaa agaaaatttc cttaagcatg ataatacgac catctttaaa agaggagatg
     4381 tagtgattgg taatgatgac tttggaaagt ataaagggga attacagttg atacttcaag
     4441 attgtgcaga tgaaagaaaa aataaggtgg gtagaataaa ggaagacgag ataatacttt
     4501 tagactttat tgaaccttgg agtaaattta agattatttt agagggagag taaagtgaag
     4561 atagacatat taataaaatg tggaaaaaca gtagataaaa gtataattga tatagctata
     4621 ttagacgata aaataataga agtaaaaaat cagatagatg attctaagta tcaggcaaaa
     4681 caaattataa acttagatgg agagaaattt attagtgctg gttggataga tatacatacg
     4741 cattgctacg aaagactaga cctttataag gattatccag atgaggttgg aataaaaagt
     4801 ggagttacta ctgtagtaga tgcaggaaca acaggagctt tagatatagg taaattttat
     4861 gaagatacta aaaaatataa aacaaatgta tatgctttaa taaatattgc taagcaggga
     4921 ataacatctc aagatgaact ttcaagtatg atgaatattg atgaatatga acttaaaaga
     4981 gcagttaaaa aatacaaaga ttttgttgtt ggaatcaaag ctagaatgag taaaagtgtt
     5041 gtaatttcaa atgatgttga acctttaaaa gtagctaaaa gaataaaaaa tgaacttaat
     5101 ctacctatga tggttcactt tgggagttct cctccaacga tagaggatat atttgactat
     5161 atggaaaaag gagatatatt aacccatata tataatggaa agccaaatgg aatacttaga
     5221 ggcaatgagg ttaaaaaaga aatcatagaa gctagagaaa gaggtataat ccttgatgta
     5281 ggtcatggaa ccgaaagttt tagtatggat attgcaatga aatctaagga tgcaggaatt
     5341 tttccagaca ctataagtac agatatatat attaaaaata gaataaatgg tccagtgtat
     5401 aatctatcta caacaatgga aaagtttata tatatgggct attcacttga agatataata
     5461 gataaagtta ctaaaaatgc agcagatgca ataagtttaa aaaataaagg tttaataaaa
     5521 gaaggatatg atgctgattt aactatattt gatgtggtta atgaagaaaa agaacttcaa
     5581 gattctttga ataagtcagt aatcacaagt acgtctataa agccaagagc agtagtagtc
     5641 aatggagaat atttaaatat aggtaagtct ataggagaga atgagtagaa gggagaaact
     5701 attgtatgag tagtatattt gaaaaatact ctgtaagacg tgttataaat gcttcaggca
     5761 aaatgactat actaggagta tcaacagtag ataaggaagt tattgaagca ataggtgaag
     5821 ggtgtaaaaa tttctttata atagaagaac ttataaataa aacaggtgaa tatatagcaa
     5881 atttattagg gtgtgaaagt gcactaatag tttctagtgc atctgctggg attgcacaga
     5941 gtgtgggagc aatgatagca aaagatgata tgagtctagt ttacaatgta aataatccta
     6001 agaaacaagt taaaagggaa ataataattc caaagggaca taatgttgat tatggagttc
     6061 cagtagaagt aatgataaga cttggaggag gagaagttgt tgagtccggt tatgctaata
     6121 tgtgtaacaa ggaacatata ataagagaga taaatgaaaa tacagcagca attatgtata
     6181 ttaaatctca tcattgtgtt caaaagagta tgctaagtgt attagatgca gtacaagttg
     6241 ctaaagaata taatttacct ttgatagtag atgcagcagc agaagaagat ttaaatgtat
     6301 attatgaatt gggagcagac ctagtgatat acagtggtgc taaggctata gagggtccta
     6361 gctcagggtt agtaataggt aagaaaaaat acatagataa tgtaaagctt caatcaaagg
     6421 gtataggaag atctatgaaa ataggaaaag aaaatattgt aggtctaaca tgtgcaattg
     6481 agagatatat aagtaatgat aaagtaactt taaaagaaat ggaggacaag ctaaatccat
     6541 ttatagacaa gataaatact ataaagggag tgagtgctag tataactagg gattcagcag
     6601 gtagagaaat acttcgtgga gaaattgact ttaatgaaga tataataaat aagtcaacac
     6661 aacaaattat aaatgaactt agaagtggag agatagctat atacactaga gactataagg
     6721 ctaatgaagg caagattgag attgatataa gaagtgtaac tggaagtgat ttagatacta
     6781 tttataatag gataaaaaat atagtagaaa gttagagtgt agagtaagtg acattataat
     6841 gatttaataa attaaatttt accaatggag gggataaata tgaattgtat accacaatat
     6901 tataaaaata gagtttgttt aaatgttctt gcaggaagca taaaaaatgc taaagaagta
     6961 tatgatgctt gtgacgggca tgtattgata ggagtactat caaagaatta cagtactgta
     7021 gaagcagcta ttgaagatat gaaaaaatac tcaaaggaaa tagataatgc aatatctgtg
     7081 gggcttggag ctggagaccc aaatcagtca aatatggtat ctcagatttc taaagaactt
     7141 cagccacaac atataaatca agtgtttaca ggtgtggcta cgtctagagc attgcttggt
     7201 caaaatgata gtataataaa tggattagtt tctccaactg gtaaaccagg atttgtaaaa
     7261 atatccacag gtccattaag ttcaaaagaa aaagatggta tagttcctat agaaacagca
     7321 atagctatga tgaaagatat gggaggaagc tcaattaagt tttttccaat gggagggctt
     7381 aaaacaaagg atgaatatat agaagttgca aaagcttgtg caaagcataa tttttattta
     7441 gaaccaacag gaggtataga cttagataac ttcaaagaga tagttcaaat tgcacttgat
     7501 gctggtgtag aaaaagttat acctcatgtt tatacatcaa taattgataa agaaacaggt
     7561 gaaacgaaag tggaagatat aggagtactt tataaaataa tgaaagaatt attgggatag
     7621 atttatgaat atattagctt ttggtgaaat aatgatgaga ctttcagtac ctgactacaa
     7681 gttcttaaca caaaccaatg aactaaatta tataattaca ggcacgggat taaatatatt
     7741 atctggactt aaaaactttg gatacaatac ctatatgttg acaaagttgc ctaataataa
     7801 tgtaggtaaa gcatcaagtg caaatataag aaagcttgga gtaaaagatg actttatcac
     7861 gtatggaggg aatcatatag gagtgtattt tcttgaaaat gggtatggag aaagaccttc
     7921 tgaagtgact tatttgaata ggttaaatag ttcattttgt gagagtaaat tacaagatta
     7981 tgatattgat aaatgtttag aaggaatgga tatagtccat atttgtggta ttgctatgca
     8041 acttacagat tcagttaaag agattgcttt agaactagct agaaaatctt atgaaaaggg
     8101 gataaaagta ttctttgatt ttaattttag acaatcatta aatccagaaa ggacttatga
     8161 tgatttagta aatgattata agaaaatact accatattgt catgggctgt ttgctagtta
     8221 tagagatata aataagatac ttaatataga tagtctaaat ataaaatcta gttcagaatt
     8281 gattaaaaaa gagtatgata tagatattgt agctggaact atgagaagat ttgatgatat
     8341 gaataataga tttataaagg gatatatatc aaatgatgat ggttattttg aaagtaggga
     8401 gtataagtta gaaatatatg acagggttgg tgccggagat ggatatgtag caggaattat
     8461 atattcatat ttggaaaata tgaagagtga agatatagtt gagtttgggg ttgctagttc
     8521 ggtgttagct catacaactt atggggataa ttctttagta agcacaggtc atgtacttag
     8581 actgatgaac aatgaaaaaa tagatatatt gagataatac atttgtatat aataaaattt
     8641 ttatcttttt tagacacatt tttgacttgc ttatatttgt taaacagagt tatttttaat
     8701 gattataaat attaaattaa aagttaaata ataaaatagt agaaaatagc attaactata
     8761 gaaaaaatgg ttaatgctat ttttatcgtt aatattgatt aattttatat tatagaaatg
     8821 aacttttcta atgctggatt tgtatttttt ttattccata caactacaac ttcaattcta
     8881 ttttctactt cttttatagg tactattgca atatcacgtt ctatcattgt atctgtaaca
     8941 caaaatgaag gtaaaatgga tattcccatg ttagaagata ccaatgattc aatagtatta
     9001 agattatttg aacgttgtac cacattaggt acaaaccctg cttcacgata tctattctgt
     9061 atcatagatt tttgtccata ctcatcccct gtttccttta caaaaataaa tttatcatgt
     9121 tgtaattcaa ttaaatcgat agaatcctca ttgtaaagtg gatgaccagg aggtaaaaga
     9181 gctaccaaag aatatctttc tacagtttta tattcaaaat cttttaatgg ttgattttct
     9241 ggattaataa caaaagcgag gtccatagaa ttatttttta ctaagtttaa aagttcggtt
     9301 atttcattag tacaaatatc catagatata ttagggtatg tattagaaaa ttcttttaaa
     9361 tattgttgaa gtttgttttt ttcatatcct attaaggttc caatgctcaa agttccaaaa
     9421 aaaccatgtg caaattgttg tgtttttgca atagcatcat tgatattttt tattatagta
     9481 cgtgcctcag ataaaaaaac ttttccagct ggtgttagtt caacatgacg tttacttctt
     9541 gtaaatagag ttacatttat ttgttcttct aaagctttta tttgctgtgt aacagcagtt
     9601 tgagaaatat aaaatttttc tgctgttttg gtaaaactta gattttctgc tacagatata
     9661 aaatattcta attgtttagt attcaaataa tcacccctat ttaaacttga ttgtttgtaa
     9721 accttattta tctataatta aagcttatta tagtataaga aaatttactt ttaaagaata
     9781 ttagaaaata tttgtataaa aatatatgtt ttaagtatat ctttacatta gattttataa
     9841 atttattaat aagaataact tatatattcc aatgaaaacg atattggatt taagtaagtt
     9901 tgtctcaaaa aatgaaagta tatcaattga taatacttta aataagaagg gagaaaaaat
     9961 ggaagataaa ttttatgcaa aaggcaacgg aaataacgga tatattaaaa atcttgaagt
    10021 ttgttccttt aataacttag atggaacttg tggaatgttt caaatggctc tgtacaaaag
    10081 agatgaaaaa tactatttat atggatgctg ttttggagga aataaaaaaa atggagtaat
    10141 gattagcgat attacagacc cttataatcc acaatttata aaacattttc aaatgttaga
    10201 ccctaaagag tatcctacaa caacaactcc caaaattcaa atagcagatg atttaatgat
    10261 agtagcaatg agttgtggaa gtggaccagg agcacttgtt gaccaagcta aattagcaaa
    10321 tattaagtgt gaagcaggaa ttagaatata cagtttaaaa gaagaccctt taaatcctaa
    10381 gtttttagga tattgggatt gtggcttaaa gcatgtaatg ggtgttcata gattcatgta
    10441 caatggtgga agatatgtac atttatcaag tgattgtgtt ggctttgaag gtctgattta
    10501 tagggtcata gatataataa atcctactaa tccagtggaa ataggtaaat ggtggagacc
    10561 agaccaatat gcagatggat atccaaatag aacttttgat gcaggagcac ctcattgccc
    10621 agaatttatg gataaaggat ggcttcatgg acctccattt gtaagagatg gaaaagcata
    10681 ttgtggttat ggaggagctg gtttagttgt attagatgtt gaagatttaa caagaccaag
    10741 atgcttaggt gaattgccat ttacgcctgc attttctagt agacttgcag gtgcaagaac
    10801 tcatacagca ttaccattgc caggaagaga tttagtcgtt gttcaaaatg agggagaaag
    10861 attccagttc tttaaaccag ataacattac agatgttcaa gctatgaata atatacatat
    10921 ggttgatgtt agtgacccaa caaaaccaac attaattgct caatttccat atcctgaagt
    10981 tccaaaagat ttcccttatc ctaactttaa tattgcagga ttaggaaaac cagggccatt
    11041 tggtccacat aatcttcatg aaccaatgga taataagcca tggttagagc aaagaggaga
    11101 tagagtatat tgctgttatt tccatgcagg gctaagggtt tatgatgtat cagacccata
    11161 ttatatcaaa gagctagcat attttatacc accaaatcca aataaaacac cagaagaatc
    11221 ttatttccca ggattcccag gaccacgctt ggcagtaaca gaagatctta tcgttgatga
    11281 tagaggctac atcatcatag atgctttaga tgatggattc tatatattaa aaatgaaaga
    11341 agattaaata aatactaaag agggagagtt gaaaatgaaa atactaggta tttcttttgg
    11401 aacaaaaaat ggaaataatg acacaatgtg taaagttgca cttaaaggtg ccaaagaagc
    11461 aggggctgaa gtagaattta ttcaaatgtc atctattaac attaaacatt gtactggatg
    11521 ttgtgcgtgt gttaaaactt tattatctgg gaaaggtagt atgtgtgtat taaaagatga
    11581 ttttgaatgg ttgcttgaca aaatgaaaga tgcagatggt atagtagttt cagaccctat
    11641 ttttgaagaa ggggcatcag gattattcca tactataatg gaccgttttg gacccagagc
    11701 agatagagga aataacatta taggaacaaa agttgcagaa gctataggag gaaaaattcc
    11761 agattcaaga atgcttaatg ataaagtaat atcatttatg ggaataggag gttctgactg
    11821 gggtacaaga gttcaatgtg aacatgctat gctagctctc ataccaatgt ggaaagttat
    11881 agataatgct tggttcccat gggcaaagga attagtaatg gatgatacaa ggcttgttca
    11941 agttcatcaa attggtttaa atatagttga agcagcaaaa gattttgaaa aagcttcata
    12001 tcaaggagaa gaaggtgtat gtccacattg ccacaataga ttattctacc ttgagccagg
    12061 tactaaaaag gcaatttgtg ctctatgtgg tatagttgga gagttagata ctataagtgg
    12121 aaaaactata tttagttttc cagaagagca attaggtcat gctcatgata ctttaccagg
    12181 taagtttata catggtgatg atattaagaa aatggaaggt cattatgcag aagttcgtaa
    12241 aacacaagaa tttaaagaaa gaaaaacatc ttataatttt atagagacat taactaaaga
    12301 aaaacaagct ggagtttcat taggataata attaaaatac taagactctt ttttaaggga
    12361 tgttagatgt tataggaggc ttattgtatg aatgaaaagc caagttttaa acaaattgaa
    12421 gttattttag cagttatttt tttggtactt acatattaca aggttagatt aactccagca
    12481 tttattgtta tatttatacc actatattta agttattttg cagtatctat gattgtaaat
    12541 caaatagcaa ttaagctatt ggagataagg caagataggg aagctactaa ttacaatata
    12601 ggaaatggat agaaagaaaa aagaaatatt aacactgtct atggggattg ctttactacc
    12661 tcctttatgg gcagtattag caccttatat aggaataaaa actggagctg tagcactaat
    12721 ctgtgcagga ttatatgtaa ctaatggtaa taaacaaaaa gatggattaa aaataatgtt
    12781 tggtttttgg tgtggagatt tatgggcagt gttagccatt ttaattatgg gatatatgaa
    12841 tttcaatcaa aacctagaat tatttttaac acttagtata ttaggttttt ttgcagttgt
    12901 aattgcatcc ttatttgaaa aaataatttt cctaccatca tggctttgtg gttgagctat
    12961 aggtctaact attatgacag gtgaaaatat tattaattta caagatattt gtattcaaat
    13021 tgctgtagct atggcagtag gagtatggta cgtgggtgca ggtgtagatt tgtttacaat
    13081 atatattttg agtaaagata ggaaaaaggg aaattgaaag catgtgaata tactttgatt
    13141 ataggagaat ggctatgggt tttgaattta aaataatgag aagtttaata tatgtaggac
    13201 ttgccaagga agaatataga cctaagctaa tggactggtt atatcgtcac catattccag
    13261 atagtattag cacttttgga ccatattgta ctaaatatgc cttttatcaa gcatatccta
    13321 caccaaatga aggtgagcgt tttggtgcac gtaagatgca actaacagaa cattattggc
    13381 ttgtagatga acatatgcct gagatggcaa atagaattat gacagaatat atgcctatgg
    13441 atgttctacg ttggcaaggg tgtataccag atgtagaaaa taaaagggtt catgaaaatg
    13501 cagaaagtgg agatgcagga cgtgcagtag gtggagataa tggatgtcca ccatttatat
    13561 ttgcctttgt tccaataaac tgggaagaag actttagagg aaaaggacgt actgtacaag
    13621 atggaccaaa ctatcgttgg caatttatga ttaagtatcc agatggtatc tctaaagaag
    13681 aaggagaaaa atggttctat gatgaggtag tgccatactt tacaaactgt tgctatgtta
    13741 atcgttttgt cagtagtaaa ataatgatta attatggagc aactgctttt gaccgtgtat
    13801 cagaactatg gtttgaaggg gaagaagaat ggtataaagc tgtggttgaa gagacaaagt
    13861 cgtttattaa aaaaccagaa tgggcacaag aagaggagtt cccatattta aaaccacaat
    13921 tcaatatcgc atcagtattc ttaggtgata tagcaactat ggatgcatac tcacagtatc
    13981 gtggatatat accaatgaga taaaaattat agtttgtgat aagtagagaa ggcatttact
    14041 cttactggta gaagaagact tattgtatcc ttaaaagtag gtgaatatat agttttatat
    14101 ttaaatttat ataaaactaa gactaataaa tatgaaacaa tagaattaga ataatttgat
    14161 aatatttgtt aaaagagcat ttagaatgtg tttaaagatt aagatattcc ttttatggta
    14221 gcagatagtt gaaataataa aactgttatc atgaaaggaa tatttttatg tataggaaat
    14281 ttttaaaaat aaaagttaaa tatgtttaag aacatcttaa agtgaaagaa tcattgttag
    14341 aaatatgtag acaatatagt ctttataacg tatcatatta acaagtttac acttggatta
    14401 tgaaataaaa agagtaattc caaaggttgt aaatactatg ctgactttga tacttcaaag
    14461 tttaggtgat tttgtaattg ttaatagaaa tacgtttaat gagcataaag aatttatcta
    14521 ataataggag aatataatgt atacaattgg acaagtatct aaatttttag gaatatctag
    14581 agatacacta aaattttatg aggacaaagg attagtaaat cctaagaaaa atgatgaaaa
    14641 tggatataga atatataatc aggttgatat ctatgatatt gcaacaataa acttttatag
    14701 agaaattgat atagaaatta aaaagattca agaaataaga aaaagtaaga gtataaacaa
    14761 tttagaactc ttactagaag agaaggaaca aatcatacta aaagagatag aatataaaaa
    14821 gcttctctta aaaaaggttc agttagtcaa agaagattgt gacaaaatca aaacatattt
    14881 tggaaaatac acaataaaag aaatgaagcc tttggaagta aaaggtaaaa taaaacatgt
    14941 tactaactat gaggaatatg aagccctaaa agagaataca aacaacttaa aaaaagcagt
    15001 tacattaact gatttaagaa gagtaataaa ttttaatgaa gaaggtataa tagaagaaaa
    15061 atttataatt gtaagggaag tagatgacta caacaaaatc atagaaggtg aagttctatc
    15121 tcatcccaat tgtatttata ctattattga agatggcaga tggtcaactg gaggagaaaa
    15181 tattgataat aaagtggaag acagattaag agaaatagca atagaaaatg aatgtgaatt
    15241 acttggaata gtctatgtta atatattgct tactacttat gaagaaggga ttgagcgtat
    15301 atttttagag atttatgcac ctataaaata atataaaagc attgacctgg gacttactca
    15361 taggtttaaa ctcatgatat aagcagttat aacttatata gcaattaaat attaatttga
    15421 aatgaggatt acttatgaga acagagaaat cattaaaaag gcctatagcc agttttatta
    15481 ttctaacaaa tataattttt ttaccacttt ttttattggt agtagtgact ataatgttaa
    15541 aacttccaac agtgatatct gacattgcgt tgtgtatatc agcgtggtca tccacttttg
    15601 catttatgat attatttaaa aaaatttatc caggtaagaa atttttagta catgtaaaag
    15661 atagatttag aaataaactt aaattttcaa cagcatctat aataattagt atacaagtcc
    15721 taatagctgt agtagttata tttatattag catctaaaaa tcatggaata atgcctaact
    15781 ttacagtttc atcatggggg gcatttatat atttattttt aaaaaattta tttgcaggat
    15841 cattaggtga ggaattaggt tggagaggat ttgttcaaaa tcatcttcag aaaagatatt
    15901 ctcctttaaa agcatccata atagtaggtt tttgttgggg gatgtggcat ttaccaatat
    15961 ggtttacaac aggattcaaa gggcttgatt taataaaata tattatattt tttatgattg
    16021 caattatatc aatcagtatt ataatgacaa ctttttataa tctaaataaa actttgataa
    16081 tacctattgt aatacatcaa ctatttaatt ttttaatagg gataattaat ggagatttaa
    16141 tagagcttat aaattactat gcaatgttat atttaatatt tgcaattgca ttaatagtta
    16201 taaatccagg aaatgtatta tataaaaata ttagagagta atatatatct ttatggtaag
    16261 atataaagaa attattagaa gaacatccaa ggaggaataa acatggtttt actgattgtt
    16321 gatgcacaaa agttaataac aaacgagagg ttatataaat ttaatgaatt tgtagtaaat
    16381 gtagaaaatt taattgatac agctagaaaa aataatatag aagtaatata tgtacgtcat
    16441 gatgatggag ttgaaaatga attgactaaa ggtaaaaatg gctttgaaat atacgaaaaa
    16501 tttaaaccat gtaatggaga aaaaatattt gataaaaaag taaatagtgc ttttaaagaa
    16561 actggtctat tagaatacct aacaaataat ggtgaaaaag atattattat tgctggtctc
    16621 caaacagatt attgtattga tgccactata aaatgtggat ttgaacatgg atttaatata
    16681 atagttccag cttattcaaa tacaactgtt gacaattttt ttatgtcagc agaacaatca
    16741 tataaatact acaatgaatt tatatggaat gaaagatatg cgaagtgtat tttattagag
    16801 gaaacactta gaaaaatgaa ataaaaatag agtatgaatt gtatatgtac ataagttgat
    16861 ttcataaaaa aggatgttaa attccaagtt aaaaattgga ttaaatatat tatagataag
    16921 gcacatcaat tttattttaa attaatgtgc tttttaattg tagtaattta tttataatca
    16981 atatgatttt tattatttaa atcaattttt cttgagtttc tttgtctaaa gaaacttttt
    17041 ttatttataa aatctacagt tatattatag tatctatttt agatgagttt aatttttgta
    17101 tcaatctaaa acaaaagtta tacttaaatg aaattgaaaa cgataaaatt ctcaaataaa
    17161 ataagtattg tggaggtgat gaaagaatcc aatgataata tgaagactaa tcttaataag
    17221 aaagtagggg ttaatggtag tggacaaaga aattgtatca attctccaga ttttattaaa
    17281 tagcacattg attacaacta atggattaca agaggagtct aattcgtcaa agagacaaat
    17341 tacttataga ataaataaaa tcaatgacat gcttaaagta aaaaatgtac cacttatatg
    17401 tttaagagcc gacaaagata ttatcgttag aaaagaaaca aaaaaagcaa ttaaagaaat
    17461 tttagaaaaa aactattcta agaatactta ttattttagt aagacagaaa gacttttata
    17521 tatgtattta atgcttttta ttaacatgga ggatatttca attaatcatt ttataaattc
    17581 tataaaagta agtaggtcta ctgtaaatct tgatttcaag gatttgatac cagagcttga
    17641 aaagaagaac attaaagtta agaacaatcg tataaatgga tattacatag ttggcaatga
    17701 gatggaaatt aggagagtac ttattaaaaa cattatagaa actttgtcta atgagaaaag
    17761 ttcaagagta tttgatattt ttattaaaga atataattta gatagttttg aagaggctaa
    17821 aaaaattata ttaaagctaa taaaaaaata tgaaattact tttgttgaag atagattagt
    17881 agaatttata tatatattta tctttctaaa agcaaggatg tattcaacta gaatagttac
    17941 tcaggaaaat gtagatattc ctaatatttc agttatgagt tcaatgaagg agtataaatt
    18001 tgctaaggaa ttattagaag tatatgggag tgaagataag ataaagcctc acaatataat
    18061 gtatattagt gcttggattt taggtatatc agtaggaaat tatgaagaag atacagaaga
    18121 taaggatgta atttcaaaaa ttgtaaataa aatgatgaca aagtttggat atttaagcgg
    18181 agtttactat ataagccaag aaaaaatatt taaacaatta tattctcatt ttagacctgc
    18241 ctactataga cttttattta aattacctat ttataatccg atttgtgaaa aagtaaaaga
    18301 agaatataaa ttagtttata gaattgtaag taatgcaatg aaagagtttt gcggattatt
    18361 tggagaagaa attcaggaag aagagttagc atatcttaca atgcactttg caacactttt
    18421 ttcaaataaa aaagaatttg atggttttag aaaaagaata gcattaatag tttgttcaaa
    18481 tggcgttggt agttcagcta ttttacatgc agaacttaca aacttatttc ctgacttaca
    18541 ctttatacca atgtcggaat cagttggatt agaaaatata agtgaacctg tagatattgt
    18601 atttactact aattataacg cagagggttt aaaaacagat gtaccagtaa ttaaagtaag
    18661 ccctgttatg accacaaaag aaaaatataa gattacacgt gaagtgtata ttcaattagg
    18721 tgatattttt ttaagacaac ctaaaataga tgaggttatg gatataataa aaaaatatgc
    18781 aaatgtaacc tctgaaagta ttctttcaag tgagctatta gcttatttta cacagataga
    18841 gaattttaca tctaaggaag gagaggggcc tatgctaagt gagattacta atgaaacact
    18901 agtaaaaata aaggtaaaag ctaagaattg ggaggaagct attagacagt cagcttctgt
    18961 attagttgaa aataacaaag taactgaagg gtatgtagat gctatgatta actcagcaaa
    19021 agcatctgga ccatacatag taattacaaa acatgttgca cttccacatg ctagaccaga
    19081 agctgggtca aaagagattg caataggcat tgctacttta gatagtccta ttgagtttgg
    19141 gaataacgaa aacgaccctg taaaatatgt attttgtcta agtgcaatag acaataatag
    19201 ccatttacgt gctatgtctg agctagttga actattggaa gaagatgagt tttttaaggt
    19261 tttagatagt gctaaagaag ctaaagaaat tattgatttt ataaaaacac acgaattata
    19321 aaggaggatt taattatgta taaaatttta atcgcatgta gagcaggagt tggctcaagt
    19381 ttaatgttaa aaataaagac tcaacaaatt attaaggaaa ataactttcc aattgaagta
    19441 gaacatggtt cattggattc actaaatgga ttcaatggag atgctgtaga aacattaatt
    19501 gatgttgctg aagaattaaa aaataaaaat ttgaagtttg atataattgg gattcacaat
    19561 attgttgacc gtaatgaaat aaagacagaa ctagaaaaat tccttgcttc aaaagaggaa
    19621 taaatttaag atgataagga ggaatattta tggagtttat tattagtttg ctgagtacac
    19681 ctgctgtttt gcttggcgta gttgcatgta taggattgat tcttcaaaag aaatcagctg
    19741 ttgaagtatt tacaggctct tcaaaaacac ttatcggttt tttgatattt ggtattggtg
    19801 cctctgctat gacaggagca cttcaaaatt tcaataaatt gttccaacat ggctttggaa
    19861 ttactggtgt tatagcttca ccagaagcag ctacagcact tgcacaaggt acatatggtt
    19921 ttgcagtttc atgtacatta attcttggat tcatattaaa tttagtattt gcacgtatta
    19981 caaagtttaa gaatattttc tttacaacag gtcatagttt gttcttttct tgtgtattag
    20041 ttttaattat gaaagcacat ggatttgata atacaatcac tattttaata ggtggaacta
    20101 tcttagggtt tatgtctgct gcactacctc aattctgtca accatttatg cgtgaattaa
    20161 caggtggcga cgaacaagct attggtcact ttaacatgat tggttatggt ttatcaggtt
    20221 atattgggcg tttattttca aagcataaag atgatacaac ggagactata gaatttccta
    20281 agtggttatc aatattcaga gacttcttaa tgggtctatc catagttatg ttaatcttat
    20341 tttatattgc aacattaaaa gctggtcaag catttactca agaaattgct ggttcaacaa
    20401 attggttggt ttttccgctt atagaggcat ttacatttgt ggcaggtatg tcaatattga
    20461 tgtcaggtgt tcgtatgttc ttagctgaaa taactgcagc atttgtaatc atttcagaaa
    20521 agtatattcc aggttcacgt cctgcgttag atgttccaac agtatttcca tatgcaccaa
    20581 atgcagttat tattgggttt atatcagcat atgctgctgg tctattagca attgctatta
    20641 tggcatcatt tcctagtata tttcctgtag taattattcc agctgctcac atatgttttt
    20701 tctcaggagg tacagctgca atatttggca atacttcagg aggatggaga ggggctattg
    20761 ctgggtcatt tgttgtgggg ttattacttg catttttacc agttatacta tatccagttt
    20821 atgggactct tggtatagaa ggagcaactt tccctaatat tgattacaat gtagttggtt
    20881 cgatattaca taatatttta caatttataa aacctattta aactaaatta tataaaagta
    20941 tagtcatagt tactatgatt tcaaggagga tacatcttat ggaaaataca ttatctgaat
    21001 ttttagataa tatatctaat gaattgtcag tatttattgc ctcaatagat gagacatcta
    21061 taaataaagc atgcgaatta attcttgaag ctgaaaaaaa tcacggacgt gtacatgtga
    21121 ctggtattgg caaaccagga catgtatcgg gatatatttc atcattgctt tcatctactg
    21181 gaacgtcagc atatatattg catggtacag aagcagtaca tgggtcaagt ggtcaagtag
    21241 ttgaaggaga tgttgttatt gcaatatcaa atagtggtga aactcaagag ttaaaagcaa
    21301 ctcttaagac tctaaaagta aatggagcaa agataattgg tgtaagtggt aatgagagtt
    21361 catttttaaa aaatatttct gatatattcc tgtttgcagg tgttaaacag gaaggagatt
    21421 gtcttaataa agctcctcgt gcttcaattt tagctgaaac aataatatta caatcattaa
    21481 gtgttgtatt acaatatgct aaaggtctta atacgcagca gtatttgaag tggcatccag
    21541 caggaagtct tggaaagtct attagagagg gaatataatt ttatgaaata taaagggatt
    21601 atatgtgcaa tgataacacc atttgatgaa aatcaaaata ttaattcaca agctacatgt
    21661 caattaattg attatttgat tgaaaaggga atttatggat tatttatttt gggcactaat
    21721 ggtgaatgtc atgtattaac agatgatgaa aaagttgaat ttgcaaagat tgttattaat
    21781 catacaaata atcgtgtacc tgtttttgtt ggaacaggag gtaattcaac tcgtgaagtt
    21841 gtagatttat ctaagagaat ggaagcgatt ggagcaagtg cattatctat tattacacca
    21901 tactttgtta caccaacaca acaggaatta atcttacatt acaaaactat tgctaattca
    21961 gttaatttac ctattataat gtataatatg ccaggtaaga ctggaattaa tattgaacca
    22021 gagtcagtac gtgaattatc aaagataaag aatattgttg gaattaagga tagtagtggt
    22081 aaacttgaca acatgaaagc ttatattgaa gcaacgaaga atgagaattt tagtgtgttt
    22141 tcaggctcag attcacttat tctggatagt ctaaaagctg gtagtcaagg agctgttgca
    22201 gcaacaacaa acttcctcac agagattggt atctctatct atgaaaactt tatgaaaggg
    22261 gatttggaag aagctcaaaa atctcaaagt agtatagaag aacttcgacg tatactgaaa
    22321 cttggaacaa ttccatctgt tatgaagaaa actattgtct tgaatggaat taatgttgga
    22381 actgcacgac ttcctgtaac tgaaccaact ggggaagtac ttgaagaaat taaacgagta
    22441 gttgaaaatt ataggacaat acttaataaa agtagtgaga atagataata taaataggga
    22501 aattacactg gtatataatt aatatatata ttataaaaca tgactaaaat taatgaataa
    22561 gtaaagtggt ttgatgagta tagaggtgat aatatgaaaa agatgaatat aaccaaaaga
    22621 atgtcagaat gtggggctct tgcaattgta agagaagaaa atcttaaccg tgcatgtgaa
    22681 attgctgaag gatgtataaa aggtggtata acagttatag aaatgagtta taccctaaat
    22741 aatgctggag aaataattca agggcttaat aaaaagtatg gtgaaatact ttgtgtggga
    22801 gcaggaacag ttttggatag tgaaacagct cgtcatgcta tattacatgg agcccaattt
    22861 attattgcac caaattataa tgaaggtgta gcgaagattt gcaatagata tcagattcct
    22921 tatgctccag gatgtacatc attgactgag gcagtagacg cactaagttt aggagcaact
    22981 tttataaaag ctttcccaat atcagatttc tatggaccta aactagtaaa ggtatttaag
    23041 acaccaatac ctgatatgcc agttttagca agtggagggc ttaatattga aaacctacat
    23101 atatggttag aaaatggagt tgatgtatgt ggatttggtg gtttattaac taaaggttct
    23161 gttgaagata ttgctaaaaa tgctaaaaag attcgtgaaa tcattaaaaa tgtaagaaaa
    23221 atttaacata gggctgtatc atagtatctt ataattacta tgatacagcc tttttaatat
    23281 tattcaaatt taaaacagtt tacatttcta ttatattaca tataatttca gtaattttat
    23341 cttaattttt ctatctggaa aaaaatgatt taaaacttaa gttatgatat aatttcatta
    23401 aaagaaggcg atttttagta aatatgagaa aataatgtgt ttttagggga aaagttaaat
    23461 acagatttaa gtgaacaaga atcgataatt aagatattaa atggtgaata gggcatcaaa
    23521 aaaattacta aattaaaagt tttagaggga gaatcttagc aagtgtgtgt gaaaattcaa
    23581 tttcaaacaa aaaataattg taaatgaaat caatttatac acgttgaaga ttcactttta
    23641 tcattatatt aaaaagcaga gtgaattaaa agtgagtcta aagataggag gataattaaa
    23701 acaatgaaag aagacattag gttacatcga atgtgtaaaa aagaacaagc aacaaagaat
    23761 gaagtcatat cagaggatat tctaaaatct atgagtattg gggtcagtaa acatttttta
    23821 gatgattcat ttacaataat atggggaaat acatcattct atcaattatt aggatataca
    23881 gaagaagaat ttttagttaa attttctagt ttaaaagatt attatgaaca gaatctttat
    23941 cattttgaat ctatgaaaaa tcatttttta gaggcttatc atcatggcga aaagagtata
    24001 gaatatcaaa tttgcatttc agcaaataat ggagaccctg tttgggtaat aatgactggt
    24061 atattaattg aatcacaaga taatgagaaa tcaatggtct atatattgta ttcaaaaatt
    24121 aatgagttaa aaataaagca agaagaacta tctgatatgg aaaaagagag tacagagaat
    24181 tttaaatgga tgatgtcaga gtatgctggg aatgtttata ttagcgatat ggatacatat
    24241 gaattactat acttaaataa gcactcatgc aatactttac aggcatcagc aaatgaactt
    24301 attggaagaa aatgttatga ggtaattcaa ggtagaactt cgccatgtcc gttttgtaca
    24361 aattcgtatt taaaaaaaga tgacatatat gaatgggaat tttataataa aaaactgaaa
    24421 cgaacattta taattaaaga ccgtatgttg aattggcaag ggcgaagagt tcgtattgaa
    24481 ttatcttatg atatgtatag tgctgaatat aaattagcaa aaaaagatca ggaaagagaa
    24541 tcaattttga aaactattcc agcaggaatg gttagattag atgcaagaga ttatcttact
    24601 atactttggt gtaatgatat atttttgaat atgattgaat atacaaagga acagtttgca
    24661 gaggaactta ataatcaatg tggatattta ttttctgaag attataaacg tagtaagaaa
    24721 ctggcacaag atttgaagga gtctggggat aatgttgtat ttgaagcaaa aatatataca
    24781 cgttcaaggg aagaacgtat atggactgtt acattgtgtt atatcagtgg ggaagacagt
    24841 tgggatggaa taccatcttt ttatagtata ggtattgaca taacaaagga aagaaaacag
    24901 attgagaaat tacagcatat atcagagaaa gatgcattaa caggaattta taatcgtgct
    24961 gagactgaaa ggcagattaa gaaatatttt gaaaaaaatt taaatgttat gggtgcactg
    25021 tttatgattg atacagataa ttttaaacag attaatgata cagaagggca tatgattgga
    25081 gatatagtac ttactgatat ggcatcaggt atgaaaaaaa taatgcgtga cagtgatgtt
    25141 gttggaagaa taggtggaga tgagtttaca atttttatga agaatatttc ctcagttaaa
    25201 gatgcagaaa agaaagcaga agaactattg tatatgttcc gtcatctatt tcagaaagaa
    25261 aaaagttttc taaaagtaac ttgtagcata ggaatagcta tatatccaaa ggatggtact
    25321 acattcaagg aaatatatgc aagagctgat aaagccctgt atcaagcaaa aaatatggga
    25381 aaaaataatt atgtaatcta caaccaagat tattttaaag aattagaaga atttgattgt
    25441 tcttctcttg gtacagttat tgattcagaa aaacaatacg ctgaatatcc tgataatctt
    25501 acacgttata tattccgtat gctttatcag actgatgata tagaccaggc gattaatatg
    25561 atacttgagg ttgttgggaa acagtttgat gtaagtcgtg tttatatttt tgaaaataca
    25621 gaagatggta gatatacttc taatacttat gaatggtgta atgaaggaat ctcttctcaa
    25681 atggcttatt tgcaaaacag caattatcaa gaatatgagg attatgaaaa gctttttgag
    25741 gatgaattag ttttttattg tcgagatatc catacattaa gtcctaaact agaagaattg
    25801 ttttctagtc aaggtatcca ctctacactt caatgtgcat ataaagaaga tagagttttc
    25861 agtggatttg ttggatttga tgaatgcaca ggactacgtc tatggtcaca agaggaaata
    25921 agtacattat cattgatttc tcaaattata tcaatgtttt tacaacgtaa aaaaaataat
    25981 aaacttaatc aacagattca acaataccag aatattttag ataatattga ccagtgtatt
    26041 tgtgtgatta ataaagaaaa taatttttta ttatatgtaa ataaaaaatt taaaaataca
    26101 tattctaaat tccaaattgg acaaccttat tatcttgatt ctatagatat tcataagata
    26161 tccattatat ggaataaaaa agaagcatac ctatgtacta gcacgattta atggtaaagt
    26221 aaagtttata ttggacttaa attaaatttc gttttaaata agatattatt ttagttgctt
    26281 actgaaaaga ctagaatcta tattaatgaa ttatactaat tttataccac tattaaaata
    26341 ttgtttgcta aaaataaaaa atagccttaa aacagttgat tataaaaaat taactgcttt
    26401 aaggctattt ttatctttgt gacgaaatag acacttgcca tttagaatgt gacaatatag
    26461 tagttgttta gaaaaggcac aaagcattat actttaacta aagtaaaatg tattttgctt
    26521 ttagaataaa aacaattagg agggagctat aattgaaaaa gataatatat gtattattta
    26581 caattgaact attaatagta tctttattag gtctaaatat agttaaagat aatgaaatta
    26641 ataatatttt atataacgat acaacaagta tatctgtaat gtttaaagat tataaaaagt
    26701 taaataaaaa ttatagtaag tggattaaag atattgcaga tgaaaataat gtggctatat
    26761 caaagtatgt ttttaataat aatgaaaaat tatctatata tacgactgat acaagtttga
    26821 ataacaatat aaagttaaaa tcaggaaaat ttcctaatac taattcaaat gaatttatta
    26881 gtaatataaa cactaataat agtaatcaag taggtaaatt ctcaactatg agtaaagaca
    26941 tatcaatttt aattagggat tttggtaaaa taagtgaagt tggagaatca ggaatcttat
    27001 atatttcaac tcaaaatgaa gatacaataa ataaaatatt aagagaacta aatgtagaca
    27061 gaagtatttt tgtagcaaga ttacatgata gatatgttaa ctcaaattta tacttaaatc
    27121 cttcaataat tagagattta atactaatta tcctttgttt cttggctaca ataatacatt
    27181 attcaattag tgtgagtaga gaggcttcta ttttaagatt aaatggatac tctaagctag
    27241 atataattgc tagagttata aaaaatatac ttagaattat gattttatca tctgtcacag
    27301 cattattaat atatataatc tatattttaa aattaaatat aggtgttaga gcatgcttat
    27361 actttgctat gttttcgatt atatgtattt tgataaatat tttgatatct atattaatag
    27421 ttggttttaa tagtagaagt tctaaatatg tgctatctct aaatggaaaa aaatcttatg
    27481 gttttgtaaa tataatgcat ttcacattga aaattgtatt tgtatcattt cttatattat
    27541 ccataaataa ctgtatttta aattataata tgctacaaac tcagttagga aatctatctg
    27601 agtgggataa agcaaaaaat gtttataatc ttactcttaa agatacaggc gaacaatctt
    27661 taggaaagga agaagtaatt aaaaatgcca agattaaagg tttctataaa aatttggtag
    27721 aagaaaaaga tgctttctta atagattcaa ctaattttga aaaacttgaa gatggaagtt
    27781 acatgtatga aataaattca aagggtaaaa acccagaagt aagtcaatat ggtaaaaata
    27841 taaaaataaa taaaaactat cttaaagtta accctattta ctcaaatggc aaagaaatat
    27901 ttaatctaat cgattatgac gataagacta taaatttatt agttcctaaa aagttacaag
    27961 tacatgaaaa tgagattaaa aaagagttta gagaattatt ttattttgaa aaaatagaag
    28021 tagaaaatat gtacaataaa gagttaaata gagagttaaa taaaacaaag aagtcagatt
    28081 taaatgttaa tataatatat gtagataata atcaaagcta ttttacttat aactcccttg
    28141 ttatggatga caataggaat ctcattgatg acccaatagc aatagttgac atagataata
    28201 ttgatgattc tttttatctt tcttatatat ctagatgtgt atattttaat tctaagaaat
    28261 tagatgccct tgcagatata agtaacatta ttgaagctca aggtgtagaa gcgcatatcc
    28321 aaagtttaca tgctgtttat aatgagtatg gtttagaaat aaataaacta gaaaaatatt
    28381 tgaatagtga aatttttact ataataataa tagcaatatc aaacttaatg attacctaca
    28441 atatagtagc tagctactat gaaagaaata aatataagtt atatattaaa aaaatatttg
    28501 gttactcaac tacagctaga agtatgttgt taatagtctc attaatatta acaaacatta
    28561 ttccaatagg cattatatca acaagagtag atttaagtaa taatattatc ttatttggac
    28621 ttctaatatt agttatagaa gtaattgtat ctattgtatt agacaagatt atctcaaata
    28681 gttcatttaa taaaattata aaaggagaac attaaaaaga tgatttcatt aataaatata
    28741 tctaaaaaat ttaaagataa atctataata gacaaattta acctagaaat ttctaagggc
    28801 gagtttgtag cgataacagg aaatagtggt tcaggtaaga caactttact taatataatg
    28861 ggattattag aaaaacctaa ttctggtgat attataataa ataatataaa aaatccaaat
    28921 agtaaacaga taagaatttt acaaagggat ttttatggct atctatttca aaattatgct
    28981 ttaattgaga atgaaaatgt tgaaaataac ttgaaaattg ctttaaagta tcaaaaaaat
    29041 atcaaccata atgaaaaaat aaattacgca cttgagtctg taggtcttag aaaatacaat
    29101 aaaaaaaaga tatacgaatt gagtggagga gaacaacaaa gggtagcatt agcaagagta
    29161 atattaaaag aatgtgaagt aatatttgca gatgaaccta caggtaattt agataaaaaa
    29221 aatagagaca tagtatttga tatacttaaa agtctaaata agaatggtaa aactatcgta
    29281 tttgtaactc atgatttaga gttatcggag caggcagata gagttataaa attgtaataa
    29341 tataaactat aataaatata agaaagagat tacagctaaa tcaagtttaa taataatacg
    29401 aatagaattt aaacattaag taaattagta gtttgtaaat ataagtataa tatgatatca
    29461 taaaggctca aaagaaattt tatataataa gaaatctaaa aataacaaat ggaggacata
    29521 taaagatgga aaatttacca aattgcccga aatgtaattc agaatatact tatgaagatg
    29581 gaactctttt agtttgtcca gaatgctctc atgagtgggc attaggattg ggagctgaag
    29641 aagattcaaa tattatcaaa gatgtaaatg gaaatgtatt aaatgatgga gattctgtta
    29701 cagtaattag agaccttaaa gtaaaaggaa gttcatcatc tataaagatt ggaacaaaag
    29761 tgaaaaatat ccgtttaatc catgactcat cagatggaca tgatatagaa tgtaagatag
    29821 atggttttgg agctatgaag ctaaagtctt ctgttgttaa aaaagcgtaa tattataaat
    29881 aagctgtagt gtttagtgtt tttcagtaca gtataaaacc ataagaatct attaagtaat
    29941 aaaagtttgt ttatttatct ttatattttc ttatggtttt attaaatata aattttattg
    30001 aacaccaaaa tattctctcc tctacaaata agccaatctt atttaaacta atatactttg
    30061 tactatttat actgaaattg taaatttaaa tgctatgtta tttaggtttg tatagttaat
    30121 ttatatattt ctgattttat aaattgtatt tattattata tcttgattct aaaatcatta
    30181 aaaatttcaa cttctataaa gatttacaat ttatcaaata cattatgagt atataaacaa
    30241 tttattatta cagtgctaca tttaaaataa gtcttagtat aaaggtattg ttacaagtat
    30301 aaagaaactt atgtatgcta ttggaataaa taatacctta aattataatt aagaataaga
    30361 actgttaaat aagggacttg attatcattt agttatagat aatataatat taaaaataaa
    30421 gcttaagagg agaaaaaata tgatagatat acataaaaat atatatgata ataagttatt
    30481 tgaggagcta aagattgatt gtaagaagtg ttttggactt tgttgtgtag ctctttattt
    30541 ttctgcttca gatggatttc ctatcgataa ggaatcagga aaaccctgta ttaatttaca
    30601 accagatttt aaatgttctg tacataatag tcttatgaag cgaggtttta aagggtgtac
    30661 tgcatatgat tgttttggtt caggtcaaaa agtagcacaa gtaacatata aggggataga
    30721 ctggatgcaa tcaccagaat taacaaatca aatgtctgaa gtgtttttaa ttatgagaca
    30781 attacatgaa atgttatggt atcttaaaga agcatctgta ttaaatatat cagatacaat
    30841 caaatcaaaa attgatttaa taattgaaga aactgaaaag ataactaata tgagcccaga
    30901 acagataatt aatttggata tcatatcaca tagaacaaag gttaatttat tattatcaca
    30961 agcgagtgag tctgtgatgg gaaaggttaa atcatttatc aagacttcta ctcttaaaaa
    31021 catgaaaaaa ttaagtaaaa atatagattt aataggagca gaccttagaa aaataaatct
    31081 aataggagca gatttaagag gtagatttct aatagcagca aatttaagaa atacagattt
    31141 gagtggggca aatttaatag gagcagactt aagagattgt gatattagag gtgctaatct
    31201 tgaaaatagt atatttttaa cacaattaca agtaaatact gcaaaaggag attctagtac
    31261 gaagttacca gcttcattaa tacgccctaa atactgggaa aaataattaa attaatacat
    31321 aattttacaa aatatgaact tttttgactt taattcatat tatacattag agattcttta
    31381 caagaatttc ttaatataat atagggagag atataaaaat gagttatcaa aattataaat
    31441 gtgaaagaga ttatgtgtat gatgattata aatacaataa gtgcaacaag tgctataatg
    31501 attatgagga aatgacacat gttcatgaat attcagaaag tgttaagtta gcagaagaat
    31561 gtgaggatag acataaccac cgtgcagcag gggttactgg tgaagttata ccaataaatg
    31621 gaggtacaaa tcatgttcat aaaataaatg ataatgtaga tttccttgac catttccata
    31681 agatatgtgt tacaacaggg ccagcaatta gaatacctgg aactgataaa cacattcact
    31741 taatatgtgg agaaactact gttaatgatg gtcattgtca taaattcctt tttactactc
    31801 aaatagaagc acctttagta taagctgatg tgaattattg tagtgaattt gctagattat
    31861 agtataataa tatataatag ataatattta aacttaatga ggactatgat atttaacctt
    31921 cgtagtcctt tctaagtttt gtataaaaaa gtgttatgca attaaaagtg atatagggag
    31981 gaaattttta tgaaatctgg aataattctt gaaatgaaac aaataaattc aacagaatat
    32041 gacgaaatgt tgcaagtctg ggaatcatca gtaagagcaa cgcatgattt tttaacagaa
    32101 aaagatattg aatctttgat accattagtt gagattggac ttaaagaagt agaaaatata
    32161 gtttgtataa aagataatga tataataaag ggctttatag gaatagataa agataaaatt
    32221 gagatgctct ttatagaaga taaatataga ggaaatggta ttggtaaaaa attaataaaa
    32281 tatgctatag ataaatacaa tgtaaaatat gttgatgtaa atgagcagaa taaaaaagct
    32341 gtaggatttt atattcactt aggatttaaa gtgtttgata ggtctgaaat agatggacaa
    32401 ggtaacccat ttccaatact acatatgaaa ttaatttaaa gttatacttc tcatttattc
    32461 gcaaaaatat ttacttaaat ttttatacaa ataaaagtcg tgacagtctt aggacacgac
    32521 ttttttacta cactattaaa ttgaaattta tctgttctat attatgatta ttagatgtag
    32581 tttttttatt ttaactatgt acttaaatac caagtatacc catacctatc aagtacttca
    32641 gcagcgccat cattttgatg tggtggtggg tgtggtaaaa attcttgtac aagtgtacca
    32701 ccttcggcaa gttttacaga tatttcttct atcgcttctc gagatggtag aacaacacaa
    32761 atcgccatgc cagaaattac aacaggcttg tctgtatccg cacccattat acaaccttcg
    32821 tattttgtga aagtaacact tgcgtgcata ataggattgt ttggattgct gtctaatcgc
    32881 gcaaatatag tttttcctcc tccgaaagcc tcagcataaa aattaaatgc tttttcacaa
    32941 gtaccattaa atattaaata tggagttagc atgaccaata tatcctcctt gatatagaat
    33001 aatttatagt tgcacctgat aatctaaatt atgatttagt aaattgtatc atagggaaac
    33061 cttgaagtat ataatttttt attattatat tatacaaatt tattaattta aaaaaatgtt
    33121 atatgtacgg aaggtttaca ggataaaata taataaaaag attaaggtga cattataaaa
    33181 aatgtatatt gacattgacg ctacgtaatt gtatatactt tgtcttaaag gagatggtaa
    33241 atatgaatat tatagaaatt cgctcagata aaatatacaa gaagataatg gatgcaccaa
    33301 taaacaaaaa agaagatata tacagatatg aattgatgaa gccttttgaa tttaagtgga
    33361 agtgtatgaa tgttccaata gttgctagac agaaaggtgg atatgatgta attatagcaa
    33421 gtgaaatgtt aggggtttta tcgcctaagg atattgatga aaagcaaaaa aagaatataa
    33481 atgtgttatc tgctgataaa atttgggcca cttgtaaaga aaccatagaa aactctataa
    33541 atgcttttat aaaagaaggg tatgatttaa acattaagga ctataaatat tcaatattat
    33601 tggcgaatcc aaatagtcct tatacaatat taagtgatgg atactggggt gatggtggga
    33661 ttcctggata tatatttcta tcattggttc ctaatgaata tactatcaat agattaccag
    33721 tattaatagc acatgaatgt aatcacaata ttagatttca gtttatagag tggaataata
    33781 atataacatt agaagaaatg atgataaatg aaggtcttgc agaaaatttt gcaacatgga
    33841 tgtttggaga ggaaatgtta ggaccttggg tcagtagaac agatatcgaa acattaaata
    33901 cttatataaa gccaataata aaaagtgctt taaaagaaac tggatttcaa aatataacat
    33961 cttatcttta tggtgatgat atagctaaaa tgcaaggata ttttccagta gggttgcctt
    34021 attgtgcagg atatgcttgt ggatattata tgattaagta ttatttagaa aagacaaata
    34081 aatcaataat cgaagcgact ttattgcctt atagtgagat aatcgaagca gtaaaagagt
    34141 tttgggaata aattttataa attgcataat ttgaaataaa ttatgcaatt attttatttt
    34201 taagaaaaaa taaattgaca ataatttata agaaatatac aataatatat ataaagaaat
    34261 atttcaatat ttaaaagttt taagatttca tatctagagt acctaacatt acatgttagt
    34321 ttcttgttac ttatctaatt actttatatt tcgtagttat atataaaatt actataaaaa
    34381 atgaaatata ttaaacaatc aactaaaaaa tgaaatatta ataaaaaaaa attcaaatta
    34441 taaatattaa atgaatataa ttaaaacaaa tatataaatt taagtataac actcacattt
    34501 taatgtattg tgaaattttt gttgaacttt atgtaccata acttataaaa tttatgtcat
    34561 gttatatttg aataagtaat aaaattgtag atgtaaatta atctacaaaa tttatatgaa
    34621 atggagttga tgctatgaca gaattaatga ttataagtgg gttagtacta ttgatttgta
    34681 taacttctag caaggtattg tataaatttg gagtaccaat attattgatt tttatcttac
    34741 tagggatgtt atttgggtct gatgggatgg taggaatcta ttttgataac tatgaactga
    34801 caaaacaatt atgttctatt ggactaatat ttataatgtt ttttggaggg tttggaacaa
    34861 actggaatat ggctaaacct gttgctatac cttctatatt aatgtcttca ctgggtgtaa
    34921 taattacggc tggcgttaca ggtatgtttt gccacatggt aataggaact agtttacttg
    34981 aaggtttatt aattggggca attgcagctt caacagatgc tgcatctgtg tttgctgtgt
    35041 tgcgttctca aaaactaaac ttaaagggtt cacttgcatc attattagaa gttgaaagtg
    35101 gaagtaatga cccaatagcg tatatgttga cactaattct tttgacattg atgaataatt
    35161 ctggggtaga acttattgca ccaatggtaa taaaacaaat agcttttggg ttagctatag
    35221 gatttatact agcaaaatta tctgtttata ttttaagaca ttcaaacttc gagatagaag
    35281 gattttatac tatatttata acagctattg caatactttc ttatgcattt agcgagtatc
    35341 ttggaggaaa tggatattta agtgtttaca tttcaggtat tattatagga aatagtagga
    35401 tacctcttaa aaaaagtttg gttcatttct ttgatggaat ttcatggatt atgcaaataa
    35461 tgttattctt tatgttagga ttgttatctt tcccttctaa gttaccaaat gtatttggta
    35521 tagcaatggc catatctata ttcatgatag ttattgcaag accattagca acatttatag
    35581 ttctagcaaa atttaaattc tcaaataaag aaaaattatt catatcgtgg gtaggtctta
    35641 gaggtgcggc ttcagtagtt tttgcaattt atgctgttac tcaaggagta gccatagaga
    35701 atgacatatt ccatataata ttttttatag cgttgctttc agtaggaata caaggttcat
    35761 tgataccaac tgtagccaga aagttggatt tagttgatga taatacacct gtattaaaaa
    35821 catttaatga ttatgatgga gaaattagct ctaaacttat cgaagtaaat gttgatgagg
    35881 gtaataaatg ggctaataag agtataatag attctaatat accagacgat atattaatag
    35941 ttatgataaa aagaaaggaa caggttttaa tacctaaagg ttcaaccatt ataaaaacag
    36001 gggatatttt ggtattgagt ggagatagaa ttgaagaatt aatacaatta taatttattt
    36061 tgtattgact ttaacctaag gggaaagttt aaacttaaat taatcaagtc taatgcgcag
    36121 gtgatgagat gttttctata ggaatgtttt ctaaaataaa taaaataact acaaagacat
    36181 taaggtatta tgaagatata gggttgttaa aaccagaata tgtagatgaa tttacaaaat
    36241 ataggtatta tacaacagag caattaccta aattacatca gataataact ttaaaacaaa
    36301 tgggtttatc attagctgag ataaaaaaag ctgtagaaaa tcctgaagaa gttgaaaata
    36361 ttttaatgaa taaagaaaaa gaaatgttgg atgtaattaa aagtgaagaa tataaaataa
    36421 ttaaactacg taattacctt atgaatttga aaggagaagt caatatgaat gatgtaatta
    36481 ttaagtcttt accaaaagtt aaagttgctt ctatgagaaa agtgttaaag gggtatgatg
    36541 aactttttaa tctatttcca aatgtaatgg ctgaagaaat gatgaaaaca gggtgtacat
    36601 gtgcagttcc tgattattgt tttaatatat atcatgatgg tgaatataga gagactgata
    36661 tggatgttga aatgtgtcaa gctataaatg atttaaagga agatacggat atattaaaat
    36721 ttaaagaatt agatgcagtt gaaaatgcag tttgtatatt acacaatggt ccatattcaa
    36781 cattaggaaa aacttacgca gatgcattta aatggataga ggacaataaa tataaagtgg
    36841 ttggaaatcc tagagaatct tatatagatg gtatatggaa taaagagagt gaagaagact
    36901 ggttgacaga agttcagata ccagtgaaac gcatattata atatgtctac aaagtataaa
    36961 taagaataaa aaaggtggtg tctcaaagta aactttttag tttatgagac actctttttc
    37021 atatgaaatc aagtatattt ttataattac gattatgaat acaattatga aaaaaggctt
    37081 atctctattt ttagacagcc tcttattttt ttattataat agttttctaa agatgctcta
    37141 tttataaatt tttattaaag actttataaa taattgtatt taatatacaa aatatatata
    37201 aatattgatg ttttatatat aaagttgtat atttttttaa ggatataaag taaaaaaaat
    37261 atacagtatc aagaaaaaaa gatactgggt atttaaagta atactttatt taattttaag
    37321 taaatcgggt atctacatat atagatacta atttcaaaga ataggagggt gtttatatgg
    37381 aaatattcgc aataggacat tcaaattatc catatgataa gcttattaat atgataaaaa
    37441 aatatgacat aaattgtgta gttgacatta gagaaactcc atattctaaa tataatattc
    37501 aatacaataa agaggcattt aacgaatctc ttagaaattc cggattttta tatatataca
    37561 tgggaaaaga atttggagct aaaagaacta ataaagatgt atatacgcaa gaaggttatg
    37621 cagattttga gaaagtagca aaagaagata tttttttaaa cggaatagaa agattaaaaa
    37681 agggttgtca aatgggttat aggatagttt tattaggagc tatgcaagaa cctataagat
    37741 gtcatagaag tatcttggtg ggaaaagtat taaataaaga aggttttgat gtaaaatata
    37801 taatgcatga aggaaatttg gcatatcagg aagatataga ggaatcatta ttggataagt
    37861 attttagtga tagaaagcaa ctgagtattg acaatttatt agggagtgca ttaacgagag
    37921 aagaaatgat acaagaaggt tataagctag ctaataaaga aataggatat agaactgaga
    37981 aactaggtaa ataaaaatat acaagtagat aaaatacagt acttatctta agtaaaataa
    38041 tattatgaat ttataatagc taggcatgat gtgccaatta aaaatattct agttatttaa
    38101 taatatcatt aaatttaggg tattttttaa tagcacatca tgttgcagtt ttaaaaaata
    38161 aaatctctaa tattgccata atcaactgtt ctattaagag taggatgact agatataaat
    38221 ctaactgtaa tttttgaatt taacaaacaa ggatttgaaa ttaaaaatac tctatctcca
    38281 ttgacaatag aagtaatagc tactggcaaa atatctacaa atgtatcata tctgttttta
    38341 gcaatttgat aaggataatt tcctacaaaa acaaatagat ttaaatcata tatattatca
    38401 gtaaaccaag cccattgacc ataaactttg tcgatattca ttgtgcctaa accaagtgta
    38461 tcaaagtatg ttgctatagt taaataaata ttttccatgt tttctaaata tgaaacagta
    38521 tatcttctga aacgtatggg tgctgtggaa gaaataggat aaaccatatt tacatttaga
    38581 ttatttgcat ttaaattaac caaagtatca catcctaaaa ttagtctata aaatacaata
    38641 tttaaatgga tgcgattttg ttacatctaa agtgtaatat tttatatatt gaaaaagtgg
    38701 taaaagtttg gatgattaaa taatattata aaaaatatat aaaatgtact tattgttatt
    38761 gctacacgat agtgatacaa tattaacgaa atatagctac ttttaatagt aacggaaaat
    38821 atttatatta aattaataaa tattttaaat gtggtatcca taaaaaataa taaacataag
    38881 ataattataa agatatactg tgttcaaatg atattaaaca agatttatat tatttgaaag
    38941 cagtatatta taatgcgaac aatacaattc taaactttgc attgtcaatt tacagttcga
    39001 taaactaatt aaaaattaaa tttatataaa gagggaaaag aggaataggg atgtttataa
    39061 ggaaaaatgt tataaagtta gcaattccta taatgataga gcagacgttt gtcatgcttt
    39121 taggagtatt taatacaatg atggcaggtc atattgggga agaagctgta tctgcagttg
    39181 gaatggttga ttcaataaac aatatattta tatctgtatt tgctgcacta tctgttggag
    39241 ctactgttgt tgtagctcaa caaataggaa aaaaagatag tgaaaaggtg aatgaaacta
    39301 ttaagcaggc tctagtgtct ggagtaatat tatcctttac aataacaata ttgatgtggg
    39361 tttttagaac tggtgtaatt aatttatttt atggttcagc agaagaattg gtaaaatcaa
    39421 atgcaaaatt atacatagag tttacattat ttacgtatcc atttattgcg acacaacaaa
    39481 tagcaaatgg tattttaaga gggtgtggag atgtaaagaa acctatgtat gtgactatgt
    39541 ttatgaatat agtaaatgta acattgggat atatacttat atatggtatt gatttaaatc
    39601 ctctaggaat aaatttgcaa acaccatcct atggcataac aggtgcagct atatctattg
    39661 ctatagctag aataattgga tgtatagtaa ttataagagc tttatttaaa ggaaacgaat
    39721 ttatatctat gggtaaaata tttccattta agattgatgt tgaaactcaa aagtctatat
    39781 ttaatatagg aataccagct ggagttgaac aactactttt taatgcaggt aaattgattg
    39841 ttcaagtatt tatagttact atgggaacat cagcaattgc atccaatgca ataggaatgt
    39901 caattgcatc cataataaat gtaattggga atgctttagc tttgtctgct acaaccttgg
    39961 tgggacagta tataggacgt ggagatatta aaggagctaa gagtacttta ttatatttaa
    40021 ctaaatttgc aacaatttgt ttatttatag taggagctat atttatacca attgcaccct
    40081 ggatttcaag tatgtatact caaaatgcta gtgtaattga catctcaaca caattaattc
    40141 gaagtgattg tttagctatg gtaatatggc ctatatcctt tgtattatca gcagggctaa
    40201 agggagccgg tgatactaga tatacaatga tgactgcagt aataggtatg tggattttta
    40261 gaatttttac aggttacgta ttgggggttg tattagaatt tggagtgtta ggaatttgga
    40321 taggtatgta tactgattgg cttgttagag ggactttata ttgtttaaga ttaagaggaa
    40381 caaagtggct aaaacacaaa gttgcataga atatatgaaa tcacttttag atggataact
    40441 ctatttagaa gtgatttttt atgtagttat tatcataatt ataagtatga tatataaggt
    40501 gattaaatta cataaataaa ggctataata gtatggatat tataaattat atattgagca
    40561 gaattacata atttggcgat aaatattaga aatatatatc tatagaaaat aatatatatg
    40621 ggtatattat taaatataaa aaatattagg tatttattta ataataaagg tgggtattgt
    40681 tatgataaat aaaataggaa aaataactct ctatgtaaat aatcaagagg atgcaaagtt
    40741 attttggaca gaaaaattga actttgtagt aacttatgaa aaggaaatgg gtccaaatat
    40801 gaaatgggtg gaagtagcac caagtaaaaa tgaatttaca acatttgtgt tgtatgataa
    40861 aaatctaatg atgaagcaaa acaaagagat aaatgtatct catccatcaa taattttaag
    40921 tacaagagat attaatggaa cttatgaaca aatggcaaat aacgaggttg aggttgggga
    40981 attaatggag atgccatatg gaaggatgtt ttcttttaaa gaccaagatg gaaatgatta
    41041 tttggtcaga gaggataaat aattttacaa agtattaaat ttactcaata tctgatataa
    41101 ttagagacaa taattattat aaactagtct aggattagaa ttatataatt aaaaattaag
    41161 tgtaaatttt gagtagaggt gataaaatgt ttaagaaaat ggcagtacta aaagatatag
    41221 caactaaaat aggtcgtaaa aaagcgtatg aactattaga aatggttgaa ggtaatgatg
    41281 cctttgtagc tgaggtaaag ataaaaaaga atggaataga atctaaaaaa gaagaaatta
    41341 tgttaaaaga taatcaaaaa ataatattag agtatataga aggttaaatt ttaaatacat
    41401 ataagttgat atactaaatt tctgagatga gtttacataa aactcatctt ttttactttt
    41461 caataacttt gttttattat ttaatataca ttttatctcc ttagagttgc tgatatataa
    41521 aaattactga tataataaaa gaatggatat taatgtaata ttttatagaa aagaggggta
    41581 ttatgcttga aataaaaaat ctttccttta atgttgagtc taacaatgag gaactaggaa
    41641 taataaatga tgttagcctt tcatttgaga ggggaaaact tattgttatc acaggtccaa
    41701 atggtggagg aaaatctaca attgctaagc ttattatggg aattgaaaaa gctacaagtg
    41761 gtcagataat tttagatgga gaagatataa caaatcttag tattacagaa agagcaaaaa
    41821 aaggaatagg atacgcattt caacaaccac caagaataaa aggtatgaca gttgaaaatt
    41881 tacttacact tgctcatgga aaaccattgt ctactgatgt atgttgccaa taccttacag
    41941 atgttggatt gtgttcaaaa gactatttaa atcgtgaagt tgataactct ctttctggag
    42001 gggaaatgaa aagaattgag atagcaactc tttttgctag agatttaaag gtttctatat
    42061 ttgatgaacc tgaagcgggg atagaccttt ggagctttgg aaaactcaat gaaagtttta
    42121 agaaaataca tgaagagtct aatcagacta taatcataat ttctcaccaa gaaagaatat
    42181 tagagttagc tgatgaaata attgttctac aagatggttc agtaaaaagc catggaacaa
    42241 aagaggctat attacctgaa atcatgtgtc aagttaattc aagttgtgaa ttaatgaaag
    42301 acatgaatta attagaaagg aaggcaaatt aaatgaacaa aatagattta aatcttttag
    42361 aaaaagttgc tgaacttcgt ggaactccat taggagcata caatatacgt aaaaatgggc
    42421 aaggagtaga gagacattca actgaaaatg taactataat tcctaaaact gataaacctg
    42481 gaatagatat tatagtaaag ccaaatacaa aaggagaaaa tgtacatata ccagtaatag
    42541 taacagaggc tggtgtacag gatatggtat ataatgattt tgaaattgga gaaaatgcag
    42601 atgttgttat aattgctggg tgtggtatac ataactgtgg ttgtgatgat agtgaacata
    42661 atggtataca tagatttttc ttgcgtaaag gagctcgcct aaaatatgta gaaaaacatt
    42721 atggtgaagg tgatggagaa ggaaagagaa ttttaaatcc agttactatt gtcaatatgg
    42781 atgaggattc atattgtgaa atggaaacaa ttcaaattaa gggtgtagat tcaactaaaa
    42841 gagaaatgga tgcaaaccta ggtaaaggag ctaaaatagt tgtatttgag aaattattaa
    42901 ctcatggtaa tcaagttgca gaatctaata tgaaggttat cttagagggt gaaaactctt
    42961 ctgctcaagt agtttctcgt acagttgggc aagataattc taagcaaaag tttaatcctt
    43021 gtgttattgg ggattccctt tgttctgcac atgttcaatg tgattcaata attatgggaa
    43081 attctcaaat tagtgctata cctgaaattg ctgcaaatca tccagatgct cttttagtac
    43141 atgaagcagc tattggaaag attgcaggag accaaatact aaagatgatg acattaggtc
    43201 ttactgaaga agaagccgag caacaaatac ttaattgctt cttaaagtaa attaaagatt
    43261 ttatttaatt taatataatt aatatagtat aatatagtga ataagtctaa aatattttta
    43321 tttttaggct tattttcttt atgtaagaaa agctattaga gaaattatat tgtaattagg
    43381 atgtggaata aatgaaaaat ttacttataa gaaatgggac aataatatct ccaacagatg
    43441 gtatcaaatt tgaaaaaaga gatatcttgg ttttacatgg gaaaatatat gctattggat
    43501 tgaaccttaa agagaaaata agaaaagaaa aattacttac aaataaagag ttcgagataa
    43561 taaatgccga taatatgtat atttccccag gatttataga tgttcacacc cattgttata
    43621 agagaaaatt acctacaggt atggattctg attcaattgg aattgaaaaa ggttctacag
    43681 ttatatttga tgcaggtaca gctggtccct caaactacta tgattttaaa aaaaagtata
    43741 tggatgagtg taaaacagaa gtatattctt ttttaaatgt tacaaatgag ggactaaata
    43801 tattgaatga atcaacttct ttagatgtta taaattttga caaggttgag tcaattgtag
    43861 aaaaaaatat agaaaagata aagggtataa aagtaaaagc gagtaaattt caatctaatg
    43921 aaagtggtat agatataatt aagaaatcaa agcaagttgc taaaaaatta aatttaccct
    43981 tagtggtata tataggagag tatccatcat atatagatga agtaatgaat attttgggaa
    44041 aaggagatgt ggttacaccg gtatatggaa atagtacaaa tggaatcctt aaagaatctg
    44101 gcacattaag aaaatcagta ataaatgcaa agaatagagg agtattgttt gatgtatgtc
    44161 atggagtgga ccaatttgat tttaaagttt ttaaaaaagc aataaagcaa ggtttagagc
    44221 ctgacctaat aagtactgat atgcatataa aaaacatgaa agacgtaaac tattctcttt
    44281 taaatgttat aaataaaatt atggaacttg ggatatccct agaaaagtgt atagaaaaag
    44341 taactgaata cccaacaaag attttcgatt tacatggaaa taaaggcaga ataaagattg
    44401 gtggagaagg tgattttaca atatttacaa tggaggagtg caatgatatt ataaaagact
    44461 acaataataa tgaacttgtt ttaaataaaa aattaagatt gcaatatact gtaaaatcat
    44521 ggatgaaaag cagtgaagtt tatagacatg gatacgaaaa tactataata aactaaacaa
    44581 tagtgataaa ataataaata tatgtttaaa ttggtatata ttggataaat atctaaggaa
    44641 acaacaatta aaaaataata attttagggg aggtatttta gtatgaaagc atttgttgat
    44701 aaggatgtat gtgtagcttg tggagcatgt ataggaacat gtccagacgt ttttagtatg
    44761 agcgatgaag ggcattcagt agcaattgaa ggagaaatac cagcagattt acaagattta
    44821 tgtaaagaag ccgaagaagg atgtccagtt tcagctataa cagttaaata gagctgttta
    44881 aatttatttt taaaataaat atataagatt taattggtac ttaagctata gtattagaaa
    44941 tttctattac tatagctttt taaaatagat aaacttaata tataagatat tactttttac
    45001 aaatatggag gactgaaata tgaaaaaaaa ttatgaaata gtattattcg atttagatgg
    45061 aacacttaca gaccctaaag aaggaataac aaagtctatt cagtattctt taaatagctt
    45121 tggcataaaa gaagatttgg aaaacttaga ccaatttata ggaccaccat tacatgatac
    45181 tttcaaggag tattataaat ttgaagataa aaaagctaaa gaagctgttg aaaagtatag
    45241 agaatatttt gctgataaag ggatatttga aaataaaatt tatgagaata tgaaagagat
    45301 attagaaatg ctctataaaa atggtaaaat attattagta gcaacttcta agccaacagt
    45361 ttttgcagaa actatactta gatattttga tatagataga tattttaaat atatagcagg
    45421 tagcaatctt gatggtacaa gagtgaataa aaatgaagtt atacaatatg tactagacct
    45481 atgtaatgta aaagataaag ataaagttat tatggttgga gatagaaagt atgacataat
    45541 tggagctaaa aaaataggta tagattctat tggagtattg tatgggtatg gttcttttga
    45601 agagattagt gaaagtgaac ctacttatat tgtcgaaaat gtagagtcta taaaagatat
    45661 tttgttataa atatctttta tagacaaaaa tattacaaaa tggtgtcgca tatttaaaaa
    45721 tatgatatta tagttaagga agtcatttaa aaaagaggag gatgcactta aaatgaaatc
    45781 tattttaatt atgggtggaa gcgacttcat aggttctgca cttgctaagc gcttgattaa
    45841 atgtgggtat caaattgata tacttacaaa tggtaaaaag gaaatagatt ataatggttt
    45901 taaagaacat ctaatttgtg atagaaaagt tcgaaaagat atggaaaaca ttatcacagg
    45961 aagaaaatat gattacatat atgatatgac agcatataca aaagaagatg taagtaatct
    46021 gattgatttt ataagcatgg ataacttaaa aaagtatata gtattgtcag caggagcagt
    46081 atataaagat agtggaagaa atataaaaga agaaaatgag aaaggtgaaa atgaaaactg
    46141 gggaaaatat gggttaaata aaaaagaggc tgaagatttt attataaata gccctatacc
    46201 atatataatt attagaccaa cttacatata tggtgaaaat aataatttat atagagagta
    46261 ttatttcttt gaaaaaatag aaaaaaatga aaagatacca gtaccaaaag gaaaacaagt
    46321 tagtaatcaa tttatatata ttggggattt ggtaaaggta ttggaaagca taatgaaaaa
    46381 tcctcatgta agagaggcct ataatgtgac aaatccacag ttaatttctt gggatgacct
    46441 tatatataca tgtggagaaa ttattggcaa agaacctata ataaaatatg tagatatgga
    46501 aaaagtagag tttagagaaa gaacgtattt cccatttaga aatatagact ttaatcttga
    46561 tataaataag cttattgaac atgggcttta tataccaaac gtgttactaa aagaaggtct
    46621 tacagcaaca tataaatggt tttcagcaaa taagccaaaa atgcatgaca gaaaaatgaa
    46681 caaagttgag tctgtaatgc aaatagttta aaaaaataaa taagtaggta gatattttga
    46741 actcaaaaat taagtatttt ttaaaatttt ttatcttaat atttattttg atttttatat
    46801 actcagttgc gatagagccg agtctattaa ttgtgaaaaa atataatata agtgaaaatg
    46861 gagcattgat aaaaaatgat tcaattgaga gtaaatctga aagtgtaaaa gtagttcaaa
    46921 tttcagatac tcaaataggt agtttttatt caactaagaa tctcaaaaaa gtagctaata
    46981 agattaacac tttgaatcca gatataatag tatttactgg tgatttaatt gattactcca
    47041 ataaaaatcc aagtgtagat gaaattacaa ctattttatc aagtatgaat gctaggttgg
    47101 gaaaattttc agtatttggc aatcatgatt atatgtataa acttcctagg tattataggc
    47161 agattatgaa aaattccaac ttcaatcttt tagtaaatga aaataaaaaa ataaaattaa
    47221 aagatgataa atatattaat atattaggtg ttgatgaaat attaaatgga aatcctaata
    47281 taaaatatct ggagagtcaa atagataata aaagttttaa tttattgtta gcacatgaac
    47341 cagatttagt agatatgctt agtaaagata caatgaattt agttctatct gggcatagtc
    47401 atggtggtca aataagatta cctattaagg gagcattagt tactcctcca tatggaagga
    47461 aatatactaa agggttttat gatataaatg gaaatcattt atatgtgagt tcgggtcttg
    47521 gaagtacaaa gttaccattt agatttttta atataccaga gataatagaa tttaatatta
    47581 taatctaaca taaaatattt agaaaata
//
LOCUS       DAEMHZ010000011        47602 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000011 DAEMHZ010000000
VERSION     DAEMHZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.011"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            270..3818
                     /locus_tag="KPK72_000581"
     CDS             270..3818
                     /locus_tag="KPK72_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2487825.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKILEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFMKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYILENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4003..5937
                     /locus_tag="KPK72_000582"
     CDS             4003..5937
                     /locus_tag="KPK72_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG2487826.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNISKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6043..7038
                     /locus_tag="KPK72_000583"
     CDS             6043..7038
                     /locus_tag="KPK72_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2487827.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIRFNYVNSASELVPLLATGKVKTGIVPEPALSALMSRNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7010..7795
                     /locus_tag="KPK72_000584"
     CDS             7010..7795
                     /locus_tag="KPK72_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2487828.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7783..8481
                     /locus_tag="KPK72_000585"
     CDS             7783..8481
                     /locus_tag="KPK72_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487829.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVENKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8604..9077
                     /gene="ybaK"
                     /locus_tag="KPK72_000586"
     CDS             8604..9077
                     /gene="ybaK"
                     /locus_tag="KPK72_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG2487830.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9274..10113
                     /locus_tag="KPK72_000587"
     CDS             9274..10113
                     /locus_tag="KPK72_000587"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG2487831.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10126..10674
                     /locus_tag="KPK72_000588"
     CDS             10126..10674
                     /locus_tag="KPK72_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG2487832.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10740..11921
                     /locus_tag="KPK72_000589"
     CDS             10740..11921
                     /locus_tag="KPK72_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG2487833.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12094..12357
                     /locus_tag="KPK72_000590"
     CDS             12094..12357
                     /locus_tag="KPK72_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2487834.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12883..13566
                     /locus_tag="KPK72_000591"
     CDS             12883..13566
                     /locus_tag="KPK72_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2487835.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13618..14796
                     /locus_tag="KPK72_000592"
     CDS             13618..14796
                     /locus_tag="KPK72_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2487836.1"
                     /translation="MFIIVGQIVVISITNNYKKTLIEHDYNIAGYLYSIGVDKSKIPS
                     AFVTHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLFQSKYIKTMSIYLLVFFTMIIF
                     ILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEEGSLSKLFSSIDVMSTSLNTHI
                     LKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQEESHNSDVVEAFVQKSQSELER
                     IETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILYSFETRAEKEGKVICVNGSENT
                     LLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFETTITTIVSVKDTGNGIHVEDIH
                     HIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTISVESKLSQGSTFTISFLKLTNM
                     "
     gene            14868..15551
                     /locus_tag="KPK72_000593"
     CDS             14868..15551
                     /locus_tag="KPK72_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487837.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15548..17878
                     /locus_tag="KPK72_000594"
     CDS             15548..17878
                     /locus_tag="KPK72_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2487838.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18059..18901)
                     /locus_tag="KPK72_000595"
     CDS             complement(18059..18901)
                     /locus_tag="KPK72_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2487839.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19196..19930
                     /locus_tag="KPK72_000596"
     CDS             19196..19930
                     /locus_tag="KPK72_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487840.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            19940..21073
                     /gene="nagA"
                     /locus_tag="KPK72_000597"
     CDS             19940..21073
                     /gene="nagA"
                     /locus_tag="KPK72_000597"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2487841.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21172..21921
                     /gene="nagB"
                     /locus_tag="KPK72_000598"
     CDS             21172..21921
                     /gene="nagB"
                     /locus_tag="KPK72_000598"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG2487842.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22096..22368
                     /locus_tag="KPK72_000599"
     CDS             22096..22368
                     /locus_tag="KPK72_000599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487843.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKNNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            23028..23732
                     /locus_tag="KPK72_000600"
     CDS             23028..23732
                     /locus_tag="KPK72_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2487844.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            23734..25221
                     /locus_tag="KPK72_000601"
     CDS             23734..25221
                     /locus_tag="KPK72_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2487845.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKLPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25426..27039
                     /locus_tag="KPK72_000602"
     CDS             25426..27039
                     /locus_tag="KPK72_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2487846.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(27402..28232)
                     /locus_tag="KPK72_000603"
     CDS             complement(27402..28232)
                     /locus_tag="KPK72_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2487847.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHRLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIRPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            28305..30032
                     /locus_tag="KPK72_000604"
     CDS             28305..30032
                     /locus_tag="KPK72_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487848.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            30069..31931
                     /locus_tag="KPK72_000605"
     CDS             30069..31931
                     /locus_tag="KPK72_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487849.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            32264..32953
                     /locus_tag="KPK72_000606"
     CDS             32264..32953
                     /locus_tag="KPK72_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG2487850.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            33058..33744
                     /locus_tag="KPK72_000607"
     CDS             33058..33744
                     /locus_tag="KPK72_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG2487851.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            33761..35668
                     /locus_tag="KPK72_000608"
     CDS             33761..35668
                     /locus_tag="KPK72_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG2487852.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIAESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSMPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            36121..36810
                     /locus_tag="KPK72_000609"
     CDS             36121..36810
                     /locus_tag="KPK72_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487853.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            37246..37785
                     /locus_tag="KPK72_000610"
     CDS             37246..37785
                     /locus_tag="KPK72_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487854.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            37804..38847
                     /locus_tag="KPK72_000611"
     CDS             37804..38847
                     /locus_tag="KPK72_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487855.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            38849..39685
                     /locus_tag="KPK72_000612"
     CDS             38849..39685
                     /locus_tag="KPK72_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2487856.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            39790..40464
                     /locus_tag="KPK72_000613"
     CDS             39790..40464
                     /locus_tag="KPK72_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2487857.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLVAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            40464..41516
                     /locus_tag="KPK72_000614"
     CDS             40464..41516
                     /locus_tag="KPK72_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2487858.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            41746..42594
                     /locus_tag="KPK72_000615"
     CDS             41746..42594
                     /locus_tag="KPK72_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2487859.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            42584..44248
                     /locus_tag="KPK72_000616"
     CDS             42584..44248
                     /locus_tag="KPK72_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2487860.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            44297..45553
                     /locus_tag="KPK72_000617"
     CDS             44297..45553
                     /locus_tag="KPK72_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2487861.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            45576..>47602
                     /locus_tag="KPK72_000618"
     CDS             45576..>47602
                     /locus_tag="KPK72_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG2487862.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFIT"
BASE COUNT        18913 a         4839 c         8231 g        15619 t
ORIGIN      
        1 taattagaaa aggaatgaat aataatgaat atattacatt attacttttt gataaatcaa
       61 aaataaagtt ataattttta atattaatta agaatgatga aatatgaata aagattttac
      121 aaataaatct taagctatct tatttaattc taatacagga agaaggtaaa tgtatattga
      181 aaaaatcata gaaatagaag taaacgattt aaataaatac aaaggtttgc aaaagaagta
      241 atataattat atacaatgag gatgtgagta tgatgaacag actaaagcca agaattttag
      301 gtattagtat cttgtttatt attataatta ttttaacatg tggagctgtt ttcaagttta
      361 aatttaaatt agataataaa ttaaaagaaa ccacacatac atatttgtct gagtttacag
      421 aacacaatat aattaatctc aagacaagac ttagtggtca atttgacatg cttgaatcta
      481 ttgctaactt cataggaaac ttgaataata taaatgatga aatggtgatt gatttaatgg
      541 attctgatgt aaagcgaagt tctttaatta gaatgactat ctctacatta gatggggaat
      601 gttattctaa tgatggaatt acccataatg ttcaagatag agattatttt aaaaaagcta
      661 aaaatggaga aaagaatata tcagagcctt taaaatctat tgttaataat aaagaaataa
      721 tagttttatc taccccagtt tataaaagtg ggaaaatagt tggtgtttta agtggaattt
      781 atgattcaaa aaagttaaat gagttattag gattatctgc atttgatgaa aaagcagatg
      841 tttttgttgc aaagaaaaat ggagatatag ttgctcaaaa aacaaataaa aatagaaaga
      901 cgctatcatg tatatttcag ctttttgaca aagataattg tgatgaagat gattgtctaa
      961 aaactataaa agaaaatatg aaaaatgata agtctggata ttttaattat aacttagata
     1021 atgaaaaatt tatgttaaac tatcagccac ttggagttaa tgattggtat gttttttctg
     1081 ttgtgccaaa taaaatagta tctattcagt cagagaatat atcttatgat gtatatattt
     1141 ttgcattaga aatactacta gttatattga tttttataat atatatagca tgtattataa
     1201 ataataacac caaaattatt ataaatgaaa aagaaaaatt taaagtactc actgataata
     1261 tattaggagg ggttaaaacc tgtttaaaag atgaatatat gactatgaca tatgtaagtc
     1321 aggggttttt agagcttaca ggttactctt gtgaagattt aaataaaata tttaaaaata
     1381 gatttaaaga tatgatttat aaagatgatt tagacaagtt taattgtatt attctaaatc
     1441 aattagaaaa acaagataaa attgaagttg aatatagaat tgtaagaaaa gatggaagta
     1501 taatttggat tttggagaga agtaagtcta taaatgataa taaaggaagt gagtgtattc
     1561 agggagtttt aactgatata acagaactta agaaaataca agaagaatta gattcaaaaa
     1621 gatttgaaat acaggctatc aatgatagta ttgttggtgg aatagtgata actgagataa
     1681 atgatgattt tgatagtata tatttaaatg aaggttattt aaatatgatt ggatatacac
     1741 gcaaacaact ggaagatgag ctaaataata aattaatcaa acttattttt aaagatgata
     1801 gaacagaatt aattaatagt ataaacaaac aactcaaatt aagtgataca tttgcatctg
     1861 aacaaagagt tgaaaaaagg gatggtacta ttatatggat tttattaaaa ggaagaaaga
     1921 tgatttatga taatggaaaa tctgtacttt gttgtgttat tgtagatatt acttctgcca
     1981 aaatattaga gcaagaactt aaaaagagtg aagaacgtca tgaaatcatt ctaaagcaga
     2041 cgcaaaatat tataatagaa tgggatatag aaaaagataa aatgaatata tcacctaatt
     2101 ggtataataa gtttaaatat gaccctataa ctgaaaacgc aacaaaaaat ataattaaat
     2161 cagtagatat atatcatgat gatatggata agtttatttc attaattaaa cttacttcac
     2221 aaggtagtga atatgaagag tgtgaaatta gacttagaac tagagatact atttacgctt
     2281 ggtttagtat aaaggcaaca actttatttg ataataatgg taaaccttat aaagtagtag
     2341 gaagtttgac tgatattgat gaatcaaaac gagaagctga aaagttgaaa gaaagggcag
     2401 aaaaagaccc tcttacagat ttgtataata aaaaagcatc acaatcactt atagaagagt
     2461 acataacatt ggataaaaaa agaaaaggtg ctttaatgat tattgatatt gatgacttta
     2521 aaggaataaa tgataattta ggacatttat atggggatgc tgtattaagt gaaattgcaa
     2581 atgacttaat aaatctattt agagcaaccg atatagtagg cagaattggt ggagatgagt
     2641 ttattgtatt tatgaaagat atttctgaaa caagagatat tataaataaa gcagaagaat
     2701 tggtaaaaac atttgaaagg tcttttttag gtaaaggacg aaattataaa atatcattaa
     2761 gtgtaggaat tgctaagttt ccacaagatg gtatggacta tatgacacta tttagaaatg
     2821 cggatatagc tctttactca gccaaaggga agggtaagaa ttgttataca ttgtatgata
     2881 aaaaaattga taaagtccaa tatataacta atacaagaaa tgcaggagat agtgataatg
     2941 tagagatgtc ttttaaagaa catatcacaa attatatatt tgatatacta tatgaatcaa
     3001 gtgatataga agtggcagta aatttaattc taagtattgt aggtaaacac tttaatgtga
     3061 gtcgcatgta tatacttgaa aattcagatg ataatcgagt atgtaaaaat acatttgaat
     3121 ggtgtaacga aggtgtaacc tcggaaatta ataatcttaa aaatatttgt tacaaggatt
     3181 taggcaacta tcttttaaat tttaatgcag atgggttatt ttattgtaat gatattgaaa
     3241 atttaggtag tgatgtgaaa aaagtactta agccacaagg catacgttca atattacaat
     3301 gtaaaatttt agagggtaat gaatttagag ggttcatagg atttgatgaa tgttcatata
     3361 atcgtatgtg gactaatgat gaagtgaaca ctttgacatt tatatctaaa acgctcagta
     3421 tgtttttatt aaaaatgcgt attcagagta agctagaaga gtctttaaca attcaaaaat
     3481 ctgtgatgga taatatggat atatgggcat atgttgttga caggaatacc tatgaattat
     3541 tatttatcaa taaaaaaatg cttgagtcag caccaaacac tcatgtaggt gatttatgtt
     3601 ataaagcgat atggaataat caagataaac cttgtaaaaa atgccctatg atatatttaa
     3661 aagatggtga tgatacttat aaatcattca tgcataataa aaagtttgag gtacaggcaa
     3721 atataactac aactactttg aaatggtttg atggtaaaga tgcatgttta atatttggaa
     3781 gtgaaataac tagtattgaa gctgaagaaa atacttaaaa tactgtgtta tatatatact
     3841 taaaatcgca atttgaattt attagactta aaatgtctat ataaatcatt taagttgtgg
     3901 ttttaagtat tttttatgat ataattaagc gttaagaaca aaattaaaag atacttttgt
     3961 ataaaaaaat atttgttaaa tatatattga gcaggtgata atatggaaaa taagttttta
     4021 ccaataagca aacaagatat gatagataga ggatgggaag aattagattt tgttctagtt
     4081 acaggagatg cttatgtgga ccatcatagt tttggaacag caataatatc tagagtttta
     4141 gaaaatgctg ggtataaagt tggaataata gctcaaccag actggagaac tacagatgat
     4201 tttatgaagc taggaaaacc tagacttgca tttctagtta atgcaggaaa tatggattca
     4261 atggtcaatc attattctgt aagtaaaaag catagagaaa aggatatgta ttctccaggt
     4321 gggaaaatgg gttatagacc agatagagct actatagtat attgcaataa aataagagaa
     4381 gcatacagtg atgttgcaat agtaataggt ggaattgaag cgagtttaag aagatttgca
     4441 cactatgatt attggagtga taaggttaga aagtctatgt taatagatag tggagcagat
     4501 ttattagttt atggtatgag tgaaaagcag attgtagaag tagccaatgc acttaatgat
     4561 ggatatgacc caaaatacat tagacatatt gatgggacat gctatatttc agatacattg
     4621 gaagaaatat atgataaata tatattaatt ccatcatata aagaaatttg tgaagataaa
     4681 atgaagtatg tagaagcttt taaaattcaa tatgatgagc aagacccatt tagaggtaat
     4741 ataattgttc aacctcatgg aagtaagtat ctggttcaaa ataagccaga aaaaccactt
     4801 agtagagaag aactagatga agtatatgga ttaccatatc agaagacata ccatcctgtg
     4861 tatgaaaaat ttggaggaat accagctata gaagaagtaa aatttagtat agtaagttca
     4921 agaggctgtt ttggtagttg ttcattttgt gcaataacat ttcatcaagg tagagcagtg
     4981 caaagtagaa gtgaaaaatc aattattgat gaagcaatag gaataacaaa tttagatgat
     5041 tttaaagggt atatacatga tgttggaggt ccaacagcta attttagaag accagcatgt
     5101 aaaaaacaaa ttactaaagg tgcttgtaaa aatagacaat gcttatctcc atcaccatgt
     5161 aaaaatttag atgcagacca cagtgaatac ttacatttac ttagagctgt aagaaaatta
     5221 cctaaaataa aaaaggtgtt tgtgcgttct ggaattagat acgattatgt aatggcagat
     5281 aagaacaata aatttcttag agagttgata gagcatcatg taagtggtca gttaaaggta
     5341 gccccagaac atatatcaga agaagtttta aagtatatgc aaaaaccagc aggaaaaact
     5401 tatgataaat ttagacagaa gttttttgct ataaatgaag aattaggtaa aaagcaatat
     5461 ttgataccct atttaatgtc tagtcaccct ggttctacct taaatagcgc tatagagtta
     5521 gcagagtatt taagagatac tcactaccaa ccagagcagg ttcaggattt ttatccaact
     5581 ccagggactc tatctacaac catgttttat acaggtattg acccgcttac aatgaagcca
     5641 gtttatgttc ctaaatcaaa acgtgacaag gcaatgcaaa gggctttatt acaatataga
     5701 gcacctagaa attatgattt ggtatactct gcgttagttg aagcaggaag agaagactta
     5761 attggttttg gacatagatg tcttataaag ccaaaaaatg aaaagcctta ttttaataga
     5821 aataattcta aaaaaaatat atctaaagga acaaataaaa acaaaaaaac taatacaaat
     5881 aacagaagta ataaaaatca acaaaaaagt tcaactaaaa aaaagaaaaa acattaaagt
     5941 ataaataaaa atacttaaaa aagaaggatt attcgataaa ttaacagaaa ttagtataaa
     6001 taaatataat tattttaatg acgttacctt tgggaggaag agatgaaaaa gtatatttct
     6061 ataatattat tagtggtggt gactatggtt gttttggtag gatgttcacc aggaaaagat
     6121 aaccctaaaa ataaggagct atcagttgtc aaacaagtaa aagtagctgt tccagatgga
     6181 ctaccagcag tggcaatagc taaacttgca aatgaaaatc cagagattaa agaaggatat
     6241 gaaacagtgt atagcattga aaagacacct gaggcgattt ccactagagt gatgaaaaaa
     6301 gatgcagata tagctatagt tccttcaaac atggctgcaa tagcgtataa taaaacttca
     6361 tcatataata ttgttggaac tgtaggaatg ggttcacttt atttggtgtc aacagaaaat
     6421 ataaaagatt attctgattt agttggtaaa gaagttggat gtactggaaa aggacttact
     6481 ccagacataa ctataaaaag tcttttgtta caaaaggata taaattattc agatataaga
     6541 tttaattatg taaattcagc aagtgaactg gtaccattat tggctactgg taaagttaag
     6601 acaggtattg ttccagaacc tgcacttagt gctttgatgt ctagaaatcc agacataaaa
     6661 attataaaaa gtttaaatga ctcatggaag gaagtaagtg gttcaaaaga cggttatcct
     6721 caatctactt taattgtaaa gtctgaattt ttaagagata ataaagattt tgttgattct
     6781 tttgtaggtc aattatcaaa tagtatagat tgggcaaaca aaaatcctga agaattaggt
     6841 gcatattctg aaaaaataaa aatatctact gaatcaaaga taataggaaa atctcttgaa
     6901 agagctaatt taaaatatat tccagttaag gacatgatta aagattataa aaattactat
     6961 gagaaattag ctaattttga tgataagaca ttaggaggca aagtaccaga tgaagcaata
     7021 tactttgttg agaaataaaa aaaataaaga acaatttata gataaaatgg aggtaacact
     7081 ttcttgtgtt atcctcttat ttttatggca aattattgct ttaaaaattg ataatgatat
     7141 ttttttacca acagtaggtc aagtatttaa tagtataaaa gagattatgc taaattctag
     7201 tttctatata gatatacttt actctatggg gagatgtttt tttagttttt tactggctat
     7261 gatttttgcc attatacttt caatagtatc ttatttaaat agatttttta gaaacttatt
     7321 aaagccaatc aatgctttga cacgttcaat accaactatg atattggtag tacttgcact
     7381 tatttggttt gaaaaggata gtaccccttt tatagttgga tttactatag tttttcctat
     7441 attgtatgat aacgttttgg gcgctatatt gaatatagac aagaacttat tagaaatggc
     7501 taatgtgtat aaaataaggt ttatagataa ggtgttaaaa atttatttac cagccataaa
     7561 atttcaaatt atatctatac tagtatcaac attctcttta agtttgaaag ttgctatagc
     7621 aggagaggta tatagtcaac ctacatatgg tattggaact atgatacaga ctgaaaaaat
     7681 taactttaat acatctggaa tatttgcatg gataattatt gttcttttaa tatctgctat
     7741 tttacaaata gcccaaaagt ttttagcaag gagagctttt ttatggaaaa gataaagttg
     7801 agtattgaaa atataaataa aagatatgat agtcgtatta tatttagaga ttttaatatt
     7861 gatttttatg taaatgaggt aaattgtata cttggaaaat ctggttgtgg aaaaactaca
     7921 ctcttgaaca taataagtgg gattattaaa aatgacacca acaatctaaa tatcaaagaa
     7981 aatttaaata gagtagaaaa taagcttgaa gctagttaca tatttcaaga tgatagactt
     8041 atagactggt taacagtaga agaaaatata aagatagttg taaataaata ttataataaa
     8101 actcaattaa ataaaatttg tgatgaatac ttggaattgg ttggaattag tgattataaa
     8161 aaattctatc ctcaaatgct tagtggaggt attagacaaa gagtaaatat tgctagagct
     8221 tttatatatc catcaaaaaa cattataatg gatgaacctt ttaaatctat tgatgcaaag
     8281 aatacacagc ttataatgga taatttcaga aatatattaa gaaaagaaaa gagaactgtg
     8341 ttatttgtaa ctcataatat tgaggaagca ttgtttttag cagataggat atttatttta
     8401 ggagactctc caattagaat aaaaaaaata ttaaaaaatt ctaaggaatt ggaaaaaaat
     8461 gaagttttga agttgatata gagttataat aatactgtcg ttttaaaata tatttggtta
     8521 aaatctataa gactatatta aaatagtaca gaatattcaa aactgtgtat aatgttcgta
     8581 ttttataaaa aggaagtgaa tgtatggtta agactaatgc tatgagaata ttagattcaa
     8641 ataagattga ttataaagta atgtcttatg aagtgaaaag tgaacatgtg gatggtgttg
     8701 aggtggcaca tgatatagga agagatgtaa atgaagtata taagacatta gtaactcaag
     8761 gagttagtaa gaatatatat gtatatgtaa taccagtaca tgaaaactta gacttaaaaa
     8821 aggctgcaaa agtagctaaa gagaaaagtg tagagatgat tcatgtaaaa gatataaata
     8881 agcttacagg ttatattaga ggcggatgtt ctcctatagg tatgaaaaag ctttataaaa
     8941 cttttgtaaa tgaaagtgca aagaatttag atacaattat tgtgagtgca ggaaaaatag
     9001 gatatcaaat agaactatct ccatttgatt tacaaagact tataaaagta gaatttgtag
     9061 atgtaataaa aaaataagaa ttacatataa aatatagagc ttatattctt aataataggt
     9121 gttgtaatat taataaaatg aataataatt ttaaaataaa tattcagtta cttatgtgta
     9181 attggtataa aaatattgag aaattttaaa aacaatggta aagtgtaagt ataatcacga
     9241 atatttttta taaagtaatg gagggatgtt cgcttgagaa aaataaaatc tgaacaaatc
     9301 gtcgagcagg taaaaaaact ttgtatagaa gcgagcctat atttaggaga agatgtttta
     9361 agttgtataa aagaaaaagc gaaaagcgaa aagagtgaag ttggaaaaaa tatattgaat
     9421 atattagttg aaaatgctga aatagctaaa gaaaaaaata taccaatatg tcaagatact
     9481 ggtatggctg tattttttgt tgaaatagga caagaagttt taattgaagg agacacactt
     9541 actgatgcta taaatgaggg agtaagacaa gggtatgaag aaggctactt aagaaaatct
     9601 gttgtaagtc caattaacag agtaaataca aaagataata cacctgctgt tattcattat
     9661 gatatggtaa aaggtgataa aattaaaata gaatttgcag ctaagggatt tggaagcgaa
     9721 aatatgagta agatgaaaat gttaaagcca tcagatggat tggaaggaat taaaaaattc
     9781 ataatagaca cagtttcaga agctggacct aacccatgcc ctcctatggt aattggagtt
     9841 ggtataggtg ggactgtgga caaatgtgct caaatagcta aaaaggcact ttttagagaa
     9901 ctaggtgaat ttaataagga cgaaaatata gcaaaacttg aaagtgaact attaacagct
     9961 attaataaac ttggtatagg acctcaaggc ttaggtggta ctacaacagc tttgggatta
    10021 aacatagaga cttttcctac acacatagca ggtctacctg ttgttgtaaa tataaactgc
    10081 catgcatcaa gacataagaa ggtagtaatt taggaggaaa ataaaatgat aaaaataact
    10141 acacctgtaa atgaaattga tatagcaaaa ttaaattgtg gagatacaat tagtttgagt
    10201 ggtatacttt atacagcaag agatgctgct cataaaagat tgatagattg tataaataaa
    10261 ggtgaagaat taccatttga cgtatatggt caaggaatat attatgtagg acctactcct
    10321 acaaagccag gagaagttat aggtgctgca ggtccaacga cgagttatag aatggatgat
    10381 ttgactatac ctcttttaga aagaggtctt agattgatga taggcaaggg aaaaagaagt
    10441 gatgaagtta tagaaggtat gcaaaaatac ggagcagttt atcttgctgc aataggagga
    10501 gctggagcgt atatttcaaa ttcaataaaa tcatgtgaaa ttattgcata tgaagattta
    10561 ggagctgaag ctataagaaa gattgtagta gaagacctta aacttacagt agcaatagat
    10621 agctatggaa ataatatata tgagcaaggt cgagcaattt atgaatgtaa ataatgttta
    10681 gaaataaata tgaatatcaa aattaaaaat agttgtgtat attttaagga ggaaaattga
    10741 tgtctaagga ttatgcaaaa ttggcattag aaatgcatga aatcaacaaa gggaaagtaa
    10801 gtgtagaaag caaggtagaa gttaaaacta aggatgattt atcaacagca tatacaccag
    10861 gggttgctga accttgttta aaaatacatg aaaatcaaga tgatgtttat agatacactt
    10921 ctaaagggaa tctagtagca gtagttagtg atggaagtgc tgtacttgga ttaggtaaca
    10981 ttggtgctga agcatctata cctgttatgg aaggtaaagc tatattattt aaacaatttg
    11041 cagatgtaga cgcttttcca atatgtttaa agactaatga tgtagatgag atagttaaat
    11101 ctgtagagtt gatggagcct gtatttggag gtataaattt ggaagacata tcttctccaa
    11161 gatgttttga gatagaagag agattaaaaa aatctttaag tatacctgtt tttcatgatg
    11221 accaacatgg gacagcaata atagttgcag cagccataat aaactcaata aagttaattg
    11281 aaaataaaaa aatagaagat ttagaaatag ttataaacgg agcaggagct gctggtatag
    11341 ccatagcaaa gatactactt aatatgaatg ttaaaaatat aatcttatgt gatagaagtg
    11401 gagcattgga agctagtata gaaaatctaa actatgttca aaaagaaatg ttaaaagtaa
    11461 caaatataag aaatgaaaaa ggacctttaa aagatattat aaaaggaaaa gatgtattta
    11521 ttggtgtttc aggtccagga gctgttacaa aggaaatggt tgaaagtatg tctgagaagc
    11581 caataatatt ggcaatggca aatcctactc cagagataat gccagaagaa gcaaaattag
    11641 gtggagcatt tataatggga acaggtcgtt cagacttccc taatcaagtc aataatgtac
    11701 tggcttttcc tggaatattt agaggagcat tagatgttaa ggctaaagag ataaatgaag
    11761 aaatgaagat agctgctgca tatgcaattg ctaatacaat aagtgatgaa gaaatatgcc
    11821 cagagtatat attaccagat gtatttaata agaatgtagt aaaaaatgtt gctaaggcag
    11881 ttaaagaagc agcgataaaa actaaagtaa atagaatata aatttttata acttattaaa
    11941 aactagaaaa gtataaaaga ctagtgaaat tatgtacagt gggatgattt tattagtctt
    12001 tttattttaa atatgacaca ataatgtcat attaagcttg atataatttt aatatggtta
    12061 ggagccaaag aaaaatataa ggagtgattt aaaatggaag aaactaaatt ttgccaatgc
    12121 tgtgcaatgc caatgggaca aactgatgaa ttatatggga caaataaaga tggaagtaaa
    12181 agtactgatt attgtagtta ttgttttaaa gatggtgagt ttacagctga tataagtatg
    12241 gaagagatga tagaagtttg tatccctcat atgcttcaaa gtaataagga tatgactgaa
    12301 gatgaagcaa gaacaatgat gaacaatttc tttccaacat taaaacgctg gaaataattt
    12361 tatgcaaaat tagttatatg caaaacatga aatatggtgt tgagaataag gggtggtgtg
    12421 ctttatagca taccacctgt tttgttactt gctagttcag ttattcttgt atgcttaaat
    12481 atgttctcat ctctctttct attttaaatt gtaaaaatct aaaatatgtt atttatattc
    12541 tacttcttta tccatgtctt tttctacctc aagtcaaata caactacaaa atcatcattt
    12601 tcaattcact gtttaataga attaacagat aaagagttat aaaattttct attttttata
    12661 taaaataata caggtcaagc tgacttctag ctacttggga tagtgtatat atttatgata
    12721 gttataggag taataccaat cattagactg ttgatttgtg tagtcttttg taaaaacatt
    12781 tgttttaata tattgagttt ttgaaaaagt aaaattataa ttataataaa ttaagtgtaa
    12841 taatgtatta gaagtaaaaa taattaaaat agaggtgatt aaatgagcag tatattgtta
    12901 gttgaagatg atttaagctt gatatatgga ttagagttct ctatacaaaa aagtggattt
    12961 agtatagaca tagcaaggac agtaaaagaa gctatgcaaa tgtatgagga aaaaaattat
    13021 gacttattgc ttttagatgt atccctacct gatggtaatg gatttcagat ttgcgaaaaa
    13081 gtacgagagg tttctaatat acccattata tttttgactg cttcagatga agaggtaaat
    13141 gtagtgatgg gtcttgatat gggtggcgac gattatataa caaaaccttt taaattaaat
    13201 gaattaattt caagaataaa agcattactt agacgttata atgttgcaag taatgtgaat
    13261 gaattaagtt caaataatat tactattaaa ttacttgaaa atcgtgtttt taaaggtgaa
    13321 tttgaggtgg aacttaccac agcagaatat aaattattgt gcttatttat gaaaaataaa
    13381 aatattgtac ttactagaaa aaacatattg gacaagttat gggatggaaa tggtagcttt
    13441 atagatgaca atacattatc agtatatgta agacgattga gaaataaaat tgaagataat
    13501 cctgaaaatc ctaagtttct attgaccgtt agaagaatgg gttataagtg gaatgtaatt
    13561 aagtgagggt attaatatga agatattttc taataaagat ataaaaatat tttagtaatg
    13621 tttattatag tagggcaaat agtggttata agtataacta acaattataa aaaaacgttg
    13681 atagaacatg attataatat agctggatac ttatatagta taggtgttga taaatctaaa
    13741 ataccatcag catttgttac acatgaagat aaatatattt taaaaggaaa agaaatacta
    13801 gatacagctg gatatggatt tgatatagat gttagcttta caccaagtgt aaaattgttt
    13861 caaagtaaat atataaaaac tatgtctatc tatttattag tattttttac tatgattatt
    13921 tttatactat atatgtattt ttttaggcaa caaagtaaga tagaagaggc tagttataaa
    13981 attgatttat ttatgaatgg aaatataaat gaaagactaa atacatatga agaaggtagc
    14041 ttatcaaaat tatttagttc aatagatgtt atgtcgacat ctttaaatac acatatatta
    14101 aaagaaaaac agagcagaga atttcttaaa aatacaattt cggatatatc gcatcaatta
    14161 aaaactccac ttacagcttt aataatgtac aatcagatac tacaagaaga aagtcataat
    14221 agtgatgtcg ttgaagcttt tgtacaaaaa agtcaaagtg aactagaacg cattgaaaca
    14281 ttgattaaga atttattaaa gattacaaag atagattcaa aaacaataat tctcaataaa
    14341 aataatgtta atcttaaaaa tttaattgtc aaaattttgt atagctttga aactagggca
    14401 gaaaaagaag gaaaggttat ttgtgtaaat ggttcagaaa atacattgtt gttctgcgat
    14461 tatgaatgga tgtcagaggc tttaagtaat cttataaaga atgcattaga taatacaaaa
    14521 gagaatgata aaataacgat agagtggttt gagacgacta taaccaccat tgtttcagtt
    14581 aaagacactg gaaatggaat ccatgttgag gatattcatc atatatttaa aaggttttat
    14641 agaagtaagt tttcaaaaga taatcaaggt ttaggactgg gattaccact agttaaatct
    14701 attgtggaac aacataatgg tactatttct gtagaaagta aactatcaca aggaagtaca
    14761 ttcaccataa gttttttaaa gcttactaat atgtaagata aaatacatct tgcagtaagg
    14821 tatatagatt attattatat taatcaatta gaaaggtgag agagaaaatg gatattcttg
    14881 tggttaataa tttatgtaaa acatatggaa atggatatac aaaagtagag gcattgaaag
    14941 atgtatcctt tacagtttca aaaggagagt ttgtgactgt agttggagaa tctggttcag
    15001 gaaagagtac actattaaat ctcttaggag gtcttgatac tccaacatca gggagaatat
    15061 ttattgatgg taaagaaata tgttcaatga aagaggaaaa acagactatt tttagacgtc
    15121 gtaacatagg gtttatattt caggcatata accttatacc agaactaact gtggagcaaa
    15181 atataatatt cccattgtta ttagattata aaaaaccaga tactatttac ttagatgaag
    15241 tattgactat tttaggtctt aaagatagga aaaatcatct tccaaatcaa ttatctggtg
    15301 gtcaacaaca gagagttgca attggaaggg ctttgattac aaagccaagt attatactgg
    15361 cagatgagcc aacgggaaat cttgacagta aaaatagtaa tgatgtaatt tcacttttaa
    15421 aagtgactgc agaaaagtat aatcaaacca ttattatgat aactcataat acgaatatag
    15481 cttctacagc agatagaata ctacaggttt ctgatggtgt gcttacagac ctgggggtga
    15541 atagagaatg agaagctact tagaattagt accacaatat gcaaaagtac ataagaaaaa
    15601 aaatagaact acaattttat gtattattat tgcagtatgc ttagttactg ctatctttgg
    15661 acttgcagat atggctatac aatgtcaaaa gatacaattt attaaagatg atggaaattg
    15721 gcacatagta attagaaata ttgatgaaaa gtttatatca gaaataaata gtagagtaga
    15781 tatagctcac catggtaggg ggcaagtcgt tgacaatgga gagtttaaat caaaggagat
    15841 tttgatacaa ggtttagatg aagaagtagc aaagcaattt aacttagaaa taataagggg
    15901 tcgttatcct attggggaga atgagacttt aatagatgag gatgcagtta aaagctttaa
    15961 tctaaaaata ggagatttta tcaaaataaa attagcagat ggtagaaata aaaattataa
    16021 gatagtaggt gaatattcat caatatcaag cttaagagct acagataaac atggattaat
    16081 actttcaata ttgggtacac aagctattaa aaaatcagaa acgtacgatt attattatat
    16141 tacatttaaa gaaaaggtta atattagaaa agccacttct gatattaaaa ataccttcaa
    16201 tttaagtgat aaacaaatcg gtaaaaatga aagattactt tctattatag gtcaaggcga
    16261 ctctgacttt tctaaaccac tgtatttaac agcaggatta ctttttgtac ttgttttagt
    16321 agcaagtatt atcatgatat ataatagttt taacatgagt gtaattgaga gagttaaatt
    16381 ttttggtctg ttacgttgtc tagggtcatc aaaacatcaa gttaaaaaat ttgttgtttt
    16441 agaaagttta attttaagta taaaagctat tccaataggc ttagtattag gaagtcttgt
    16501 aactattata tcttctatat ttcttaagta tgttaatgag ttattcagct caatgccaat
    16561 tttaaaagtt agttttattg gtattgcatt tggaataatt gtagggtttt taactgtaat
    16621 attatcagct attattccag caaaaaaagc ttcccgtgta tctccactta gcgctgtacg
    16681 tgggaatcta atgtccaaag aaattttaaa taacgggaag acaagtaaag ttgcaaatac
    16741 aggaagtttt aaaattgaga cttctatggg attaaatcat gctttttcaa gaaagaaatc
    16801 ttttgtactt atgacttctt catttgccat tagtattata ctatttttag gttttagtgt
    16861 gcttatagac cttatgtatc aaggacttaa gcctatgaag ccagaagctc ctcatatatc
    16921 tattgttgac cctaatttta agtctgtttt aactaaggaa gatattaatg aaataagtaa
    16981 tatcaaagga gtaaagaaag tatacagtag aatgatgacg gatatagaag ctacatataa
    17041 tgggaagaag acaaaatcac aattaatttc ttatgaaaag tatcaattta attgggcaaa
    17101 aaaatatcta ttaaaaggag aagttaatga aaataaacta cagtcagaaa accttgcatt
    17161 agtagaagag gggcatggat ttgatatagg agataagata tatattaaaa atagaaatgc
    17221 tgaaaaagag ttagaggtag gaggtatact ttcctcaatt ccatttgagg ctaaagataa
    17281 atatattgga aatattataa catctgaaaa tacttttacg aatataacag gtataaaggg
    17341 ttatataata atggatattc aggttgataa agatactcct ggagatttaa ttgaaaatat
    17401 aagacgtgta gtagatgata acttagaggt cagagataaa agacaaagta acttagaggt
    17461 taaaaattcg ttttatacta tggcaacatt tgtatatgga tttgtgttta ttattgcgat
    17521 tataagcatg tttaatatca taaatagcat gaatataagt gttacaagta gaaagaatta
    17581 ctatggtgtt atgcgtgcag taggcatatc caacagacaa ttaagaagaa tggtaatagt
    17641 agaggcaggt tcatatgcaa tgagtggttg tttattaggt agtatactag gattaatgtt
    17701 acataggtat atgtttatat cgttagtaag ttcaatgttt catttagagt ggcaagtacc
    17761 ttttaattta ctacttatca ctgtactaac tatgataata ataactttat tatcagtaag
    17821 aaaaccagtc aaaaagattt gtgaattaga tataatagac tcaataaatg cacaataaat
    17881 atagctgttg atttaaatta aaaaaacgaa gtaaaaatga aatagaaaat atttttcatt
    17941 tttacttcgt tttttatatt ataaaatttt tgttatgtta caaaatttct ataaagatga
    18001 tgtgaatcta aacattatat gacaacgtga gcatttaagt taaaatattt taaatatact
    18061 attttagata agttccttct tcatcttgat aaattaatcc ttttttcata agcactccaa
    18121 gagctcgttt aaaatttttc ttacttgaat taaataatat agaaatatct ttagggtcag
    18181 atttatcatt aaatctcata tatccatcag aaccctctaa ataacttaaa atcttatttt
    18241 ctaaagtatc aagttcatcc tttctatttc ctctaggaga aagaccaagt tttccatctt
    18301 catatatttt tatgacatgt aaattttcaa gaacatcacc agcttttagt tcagtaaagt
    18361 actcattatg aagtattaca ccagcatatt tattgtctac acatatcatt gcagaattat
    18421 tagtttgaaa tccatataca actccagtta caatatctcc tacattgtaa gtatggtctg
    18481 ttaataaata tgaatctata tctgtagtag cagcaattct tttgcttttg tctaaatata
    18541 tataaaataa atatttttcg tctctaaata gtggataagt cttagcttta aaaggaacta
    18601 gtatgtcttt aggaagtccc atatctataa aagtaccaat gtccgtatta tcaactactt
    18661 ttaaatgtgc tacatcccca accttagcta aaggcggtat taaagttgca gcagttctgt
    18721 catcagaatc tttaaatatg aaagcattta cttcatcacc tatgtttatt tcattttttc
    18781 cgattaatct attatgaaga agtatatcgt cttttgtgtt attagtttgt ccatccaaaa
    18841 aatatccaaa ttcagttttt ctttttactg taagtttatt aaaatcacct atgtttatca
    18901 aattaaaatc atcctctcat tttatattta taatttagcc ataatatact tatgtagtag
    18961 tttgtcacag tatctatatt agtattagtg aaatttacaa taaataatat agttatttat
    19021 gtatttttca attctctagc ttcttatata gtataaactc caatttcttt tttgtcttta
    19081 ggttttacta aaatttttac aaaataagtt aaaaagtatt gaaataaaat ttcttttaaa
    19141 gtataattgg tatataccaa aattggtata taccatatcg aaaagtggtg actatatgaa
    19201 aatagtaaat aaatctagta atataccatt gcatactcaa ttaagttcaa taataagaga
    19261 gatgatagaa actggtgagc ttaaagaggg tgatgcaata atgccagaaa gagaactttg
    19321 caacatacaa aatgtaagtc gtatgacagt aaataaaacc attgttggat tagtcacaga
    19381 aggacttctt tatagggtac aaggtaaagg gacattcgtt gcaaagcaaa aaaagaagta
    19441 tcagttttca aatgtaaaag gttttactga tgttatgaaa gaaaagggag taaacataaa
    19501 aacggatata ttatcctttg agatggaact accagatgat ttagttaaaa gaaagcttgg
    19561 aataagtgat aatactacaa atatatataa gattgtcaga cttagatata ctgatggaga
    19621 gccatttgga ttagaaatag tatatctatc agaagaaatg tgtaaaggtc taacaaaagg
    19681 gatattggat aattcttcat tatatagggt tttaaatgaa aaatatggct ataagataca
    19741 aaaagctgaa caagtaatgg agccagtaat actttcagat gaagaaagta agcttcttga
    19801 aacagatgaa ggtgctttag ctcttaaact tcatagaaat tcctataata gagaaggtag
    19861 ccctatagaa tataccatat caatatttag aacagataaa tatcaatatg aaatagtatt
    19921 gagtgagtag gtgaataata tgaaatgttt aattaatgga aagataatat taaaaaatca
    19981 aatcttagaa aataaagtat tagtatttga tgaaaaaata attgatatag ctgatagtgt
    20041 accaaaagat tgtgaagtta tagatgctga tggaaaatat atatctccag ggcttattga
    20101 tattcatata catggaaaca tgggaaaaga tacaatggat tcgactgatg aatctattga
    20161 aacaatttca aaatcaatta tgagacatgg agtaacatct tttcttccaa ctacaatgac
    20221 tatggataaa gaacatgttt atgatgcact tgaagttatt aaaaaagccc aaaatagaaa
    20281 gcttgaaggg gcacaagtat taggagcaca tttagaagga ccttttataa atgagaatta
    20341 taagggagca caaaatgaaa aatttataat aaattcaaaa tacgaattta taaaagaata
    20401 taaagatgtt ataaaagtta taacttatgc tccagaaaaa gatattgatt ttgattttac
    20461 tagagaaata aaaagatgta cagatatagt tttatctatt ggtcatagta atgcaaatta
    20521 tgaccaagct aaagaagcta taaatttagg agtaactaat gtaactcaca tgttcaatgc
    20581 aatgactggt ttaaaccata gagacccagg cgttgttggt gcagcactta caactaatgt
    20641 gtacagtgag ttaatagcag atacaatcca tataaataaa gatttatttc aatttatact
    20701 aaataataaa gggaaagaaa gactgatttt aataacagat tctatagaag ctggtggtct
    20761 tgaagatggc aattattctc ttggaggtca gaaagtcata gttaagggta atgaagctag
    20821 acttgaaaat ggagcattag ctggaagtgt gttgtcttta aataaaatgg tatttaactt
    20881 cttagataat acaaacctta aagtgaatga agcaattaat ttagcttctt taaatccagc
    20941 aacatctttg ggaataaatg acaaaaaagg aagcttagaa ataggtaagg atgcagatat
    21001 agctgtattt gatgagaatt tagattgtaa aatgacactt tgcttggggg acgtagtata
    21061 taaaaatata tagtatacaa aacaaacttt aaagacagta aaaacaatat aaaaaataat
    21121 aaattaaact tacattagtt aaagtagtag ttaaaattta ggaggaataa gatgagagta
    21181 ttagtgtgta aagattatga tggaatgagt aaaaaagccg ctgaaatgat agcagctcaa
    21241 atagttttaa aaccaaattc aatactggga ctagctacag gaagtacacc agtaggtatg
    21301 tatagagatt tagtaaaaaa atacaatgat aatatagtag atttttcaga tgtgatgagc
    21361 tttaatttag atgaatatta taagttacca atatctaatg accaaagcta tgattatttt
    21421 atgaaagaga atctatttaa tcatgtaaac ataaagctag aaaatactca tctaccaaat
    21481 ggaatggctg atgatattga aaaagagtgt ctgaactatg aagcttctat agatgcagct
    21541 ggtggtatag atgtacaagt acttggaata ggtcgtaatg ctcatatagg ttttaatgaa
    21601 cctgatacta aatttgcaaa aagaactcat gtagttgaac ttacagagtc tacaatagaa
    21661 gctaatgcaa gattctttaa atcaagagaa gatgttccta aaaaagctgt aagtatggga
    21721 ataggttcaa tattgaagtc taagaaaata ttgcttttag cttcaggaga agaaaaagct
    21781 gatgcagtat ataatactgt ttacggagat ataactccag aagttcctgg ttctatactt
    21841 caacttcata aagacactat agttatagta gatgaagcag cagcaagtaa attgaatcca
    21901 aaagattata aattagtata agaatagaat gtagtttaag taaaaaatag ctatcattga
    21961 ataatcaaat ttatttaatg atagctattt ttatttaaat tttttacttc tttttattta
    22021 aattagttat tattgtttaa attacttgct tattactttt tacgaaaact cgttatcaag
    22081 aagtatccaa ataagatgtt aaaaattatt atgtttagtg ggattctatc aagcataata
    22141 aaattaccta taattgggat attaccaact ggaaacataa agaataatac aggcatcagt
    22201 gatagtaaaa aagatggaaa acaaagaaga agtaatcttg gaatatcaat ataccaacta
    22261 ccatcctttt tgtattctct aatcaataat tcagttccta gtaataaacc aataccacca
    22321 gaggaaatta aattaataaa aatgtatctt ttaaagtctt gcatataacc agtgtgaaat
    22381 tgagtataga aatcataatt taatttaacg gctaaaaaat acataagcaa tagaatgatt
    22441 aaaaataaaa gatatctaag agcttttttc attttaaatt ctcccttcag aaatacttta
    22501 ttcagtttta ttttgttgga atctgatttt atcatttagt ttaagaaatc cataatatta
    22561 gtgaagaatg aaataagtat tatatatgaa taaatatttt tagaattaag gtgaactgta
    22621 gtgcttaata taactataat attaattgtt tgaatattca ataaaaagtt tgaaaaatat
    22681 caattttgat ataaactgaa taaatacagt tgaatattaa attattattt tatcaaaaat
    22741 atgcaaagta ctacaccttc aaatatcacc taaagtaatt aaaaggcaat taaagtgaag
    22801 tagttcaagt atttctaaaa taagtttaat aaattctata ataatttatt ttttgcctta
    22861 aaattgcctt aaaatccaaa caattttaaa actaatgtac tataaaaagc tggtataatt
    22921 taaatatagt aaaaaataag atttatctta tctattggga tttaattatt taatcaatat
    22981 atttatacca tttaatttaa ataaaaagtc tattaaggag tgagcttatg agtaagttaa
    23041 tttatatagc agatgatgaa gataatataa gaaatttggt aaagacattc ttaaaaaatg
    23101 aaggtcatga tgttatggat tttaaaacgg gtgatgaact tttagaacaa tttaatataa
    23161 aggagtgtga cttagtaata ctagatataa tgatgcctgg ctcaagtggt tttgaagtat
    23221 gtacaaaact tcgtgagaaa agtacagtcc caataatcat gcttactgca agagatacag
    23281 atatagatta tattacaggg ataaccttag gtagtgatga ttattttaca aaaccattta
    23341 gcccaatgtc tttggttatg agagtaaaat ctatatttag aaggatagag tttgaaaaga
    23401 aacaaaatta tgataaatat agtaactcga tagatatgga attaaaattt ggtgatgtaa
    23461 taataaataa aaagaataaa atcgttacaa gtaaaaatgt aaatatagac cttacaccaa
    23521 atgaatataa cctattgact tatttgtttg aaaatataga tagagcagtt tccagagatg
    23581 aattattaaa caaaatatgg ggatacgata tagaagttga gacaagggct gctgatgata
    23641 cagtgaagag attacgtaaa aaaattttaa atacaaatat tttaatagaa actgtatggg
    23701 ggtttggatt taggttgaag gagaaaagtt aagatgaaga atggaaatga caagaataga
    23761 attggaaaga ttaaatccaa atttaaatta aagttctcaa agaaaaatat aagcataaaa
    23821 actaggattt tgttgagtat actttgtttg ataattatag tatttatgat aatattagta
    23881 tcttttaatg tatttcttga tacatatata aaaacaactg cgaatgaaga attgacaaag
    23941 tctacagaaa tagtagaaca tatggatagt aattttagac ccattaagct accacaagat
    24001 gatggaaagc ttcctcccaa agtattatct aattttatga gaaatgtaca agataaagtt
    24061 aggatggcag aaacacaatc agatgctgat gctatggtag ttgattctaa gtataacctg
    24121 atatttccaa caagagagga tgatttctta aaaaatgttg atgagatgga gctgattagg
    24181 gagtgtattc aaaatgaaaa attagattta aattcagatg caaatacacg gatatctacc
    24241 agaaataagg attattatat ttcaactgta aaaattacta gtatatatgg tgaacaagac
    24301 aagtatctta tactttttat agacatatca aaaactctaa atctagcaca aaagataaat
    24361 attgtattaa tatctgtaat gtgttttgct ggaatattag caatatttac agctgtgatt
    24421 ttatctgaga aaattgctaa accgataaaa gagttgtgtg agtttgcaaa aaaaatgggt
    24481 caaggagatt tcaaaagaac ctactttgac ttttcagata aagaattagt agagctctca
    24541 actgttatga ataagagtgc tgagtatctg gataagtatg ataatgaaca aaaaatattt
    24601 tttcaaaatg catctcatga attaaggaca ccacttatgt ctattaaagg ttatgcagaa
    24661 gctataaaat ataatgttat agatagtaaa catgcaagtg atataattct tgaagaaagt
    24721 gataggttaa gtgatatggt tgaagattta ttatacattt ctaaaataga taatataact
    24781 aaagattttg aacttgtaga atgtgattta agagaagtac tttcaaattg tggagctagg
    24841 caaaatgtaa gagctataaa taaggggata aaatttattt atgaatttga tgaagaaatg
    24901 gttttatttg aatgtgatga aaaaaacatt tccaaagcat ttatgaatct tatagaaaat
    24961 gctttgagat acgctaagag tgaaataaaa atagtgtgta agtataatca aaaaaatata
    25021 gttgtgataa tagaagatga tggaattgga atcaagaaag aagatttacc tcatgttttt
    25081 gaaagattct ataagggagt aggaggaaat catggaattg gattatcaat agttaaatct
    25141 attgtaaata agcatggtgg gcgtatatac gtagaaaatg gcaaaaaagg agctaaattt
    25201 actatattat ttaaattgta aaaaaattag agaaaatata gagatgtttg aagttgtttt
    25261 ttatatatat ctgttgtata ctgttgaaaa aagaacaata ttaagctgac atatattacg
    25321 atagttgaca tttaaagtaa atgcttaaat atagatacga aaggaaaagt aaaatctgaa
    25381 gtgtatttag gtttaagtaa gcttcttatt ttataaaaaa tatgtatgaa agaacagctg
    25441 atttttaata ttacaaattt agtaacggtt gtatttgaag cttttgtcat ccatatgttt
    25501 ttatcagact ttttaggcca gaaagaaggg tatataaaag ttgtaagata tgctaaactg
    25561 ggttttataa tatgtttagg attctgtaat ttaataactt taaaccctaa aatcacaatg
    25621 cctttaattt tcatccttat tttttcaaca tcatttttat ataaaggaaa tttaaagaca
    25681 cgattgttta ctactgtttt attaagtata ttctttatac tgtcagaaat tgtagttaca
    25741 agtatttttg ttttatttgt aaaagaaggc tttgaaataa tgcttgaaaa taatagtata
    25801 agagtattgg cgacaatttt atctaaaatt gtctttttgt tgacttgtaa aattatttgt
    25861 ctatttaaaa aagatgtaca tttagatatg cccataaagt attggctccc tttattttta
    25921 atacctatat ttagtttatt tctgtctgtt tctatctttg atgtaagtaa attttttagt
    25981 cttgaaagtt taaaatttct aagcttaata tctagtgttg gtattttata tattaatttt
    26041 attgtctttt atttatttaa atttattata gataaaacta aactatctat gaaatatgaa
    26101 atacttgaaa aagaaataat tcataaggaa gaattaagac ttagtaatga gtgttataaa
    26161 attattgttg agcagactga ttctgtcgtt ttcgaatgga atatcaaaga aaataaatca
    26221 tttgtatcgc aggcttggac tgaaaaattt ggatataaca atgcatgtaa gaatattttt
    26281 aaagaaatta aagataaaga cttagttcat tctgaagata aagctatatt tgaaggtttt
    26341 cttgaaagta ttaaaaaaaa gaatatgcac aatcaagcag tatatcgttt aaaaaaatct
    26401 aatggtgaat atatttggtg tagaacatca attacaagca tttataatga tgaaaatgaa
    26461 cttttaagag ttgtcggagt aattgtagat gtagatagtg atataaaaaa atatgaagag
    26521 ttaagaacta gagcagaaag tgactcattg accaatatat acaataaagg tacatttgaa
    26581 aaattagtgg aagaaactat agttatgaat acaggtgata aaaaagatgc attattcatt
    26641 attgatttgg atgattttaa agaaataaat gataattttg gtcatccatt tggtgatttt
    26701 gtactaaaaa catttgctga taagatacaa actagttttg gtagtaaaga cttagtcgga
    26761 agaattggtg gagatgaatt tgttgtatat atgcaagatt atgttacaga agtaaatttg
    26821 cataaaaaag ctaaagaatt aaatagagta ctttcggata attacactga tttgagtttt
    26881 agttttgatg cttctgtcag tataggtatt gcaaggtatc ctcaagatgg aacttcattt
    26941 tttgaattgt ttaaaaatgc agatagagca ctttatagca taaaagcatc aggtaaaaat
    27001 tcatattgtt tatttgaaga agaactatat gtacagtgaa tttgaattta gattttatgt
    27061 gagagtaaga tttatatata cttgtggctg tctgatttat aaaatatgtt actttgtata
    27121 tttttatatg tttcagtaag gttaattaac atgagcaaag gtgtaagtaa atttatatta
    27181 gtttaagttt acttacacct ttataaaaat acaaataata gattcattta cattcttaaa
    27241 tgattaaagc tctttacctt ctaaacatgc ataacatatc gttataattc cagtaaataa
    27301 gactaaaatg cccattccta agattacaat ttcatcatct attactccaa atgtttttaa
    27361 agtaacttat ttcgctttgt atcataataa tttatctcct tttacatttt aacttctgct
    27421 gagatttgag ttagatattc gttagaatct cgcacactta tatcatatag aagatattct
    27481 tcataaacaa aatccccaat acgtaaatta ttctgattaa aaaaatttaa taacttttca
    27541 taagtcttgt aaatagtttc ataacttcct ttatgataag cagttacata aagtcccttt
    27601 ggtctgattg atacaggata tttcttgtac tctttattaa cttttgtaaa taaatatgaa
    27661 tatgattcaa agtctttatt aattatatct tcacctttaa tgattgagcc aatcgtacca
    27721 taatcactca attcatagtt gtccataaag ttaatacatt catataaaaa attttcttcc
    27781 tcatctccta aaaattgttc tttatatgta acaggtgttt tgaccaagta ctcttcttca
    27841 tgttctttta aagtaatagt attcgcatct atattacagg catttttagt gaatgaaata
    27901 gtagattcca ttagagcttg aatattattt agtttttcaa tttcattttt aatatcaatg
    27961 gttttctttt ctaaaagctc aattagtaaa ttaggtgttc ttttgtctat ataagcttta
    28021 atttctttta aaggcatttt tacttcccta agtgaagaaa ttataccaaa aatgctaagt
    28081 cgatggtatg agtagtatct atatccattt tctttcttta attctggtga gaaaagacct
    28141 atatcatcat aatggaaaag tgttttttta ttgattccac aaatctttgc aaattctcca
    28201 gttgtaaaat aattttttga aacttgactc ataattataa ttcctccttg actatcctgt
    28261 tacagtatag tttatcatag tattttgtaa aggagtgtga ttatatgaaa aaattaattc
    28321 attttttaaa accatataga gttttaattg ttgtagtttt gatatttacg tttttacaaa
    28381 cacttggaac tttgtatata ccaacactta cagcaaatat agtaaataat ggtgtagtaa
    28441 agggagatat agattatatt gtaaaaacag gtttaatgat gatgatagta gctggtataa
    28501 cagctttatc agctgtgtta gtttgtaaag tatcagcaaa tctttcatct ggattttgta
    28561 gagatattag agaagcagta tttataaaat cacaagattt atcaataaat gattttaata
    28621 atataggtac agcatcaatg attactagga gtactagtga tattacttta ataggtcagt
    28681 ctgtgtttat gtttattcaa ttagtattac cagcaccaat tataactgtt tctggtctat
    28741 tcttagcata ttctatagat aaagcaatga caattattat tgttgtagta atgtttttat
    28801 tcatgctttc agcattttta gttggtaaaa agcttataaa gttgtttaaa atgatgcaaa
    28861 taaagatgga caatatgaat agggtgttac gtgaggtagt aactggagta agagttataa
    28921 gagcttttaa taggagtcgt tttgaaaaaa agagatttga tagaactgca atagattatt
    28981 ctgaaacagc aatttcaata aataaaatat ttgcagtact tatgcctatt gtaatgttaa
    29041 taatgaattt aggtatagta agtattattt ggtttggtgg gatgagagtc agtaatggaa
    29101 atatggaaat aggtcatata atggcacttg ttgaatattg tatcttaatt ttattctatt
    29161 taatcatggg agtaatggta tttatgtaca tacctagagc aggtgcatgt gcagatagag
    29221 taaatcagat attagatata gaacctgaaa ttgtagatgg aaatggtcat aaagatactg
    29281 tctcagagag aggacattta gtatttaaaa atgtaacctt ttcatatgca caatcagaag
    29341 aacctgtgtt aaataatata acatttgaag caaaatctgg agaagtaact gcaattatag
    29401 gaagcactgg ctcaggcaaa tctactattg caaatataat accacgtttt tttgaaattc
    29461 aatctggaga aatatctata aatggtcagg atattaagaa gattcctcaa aaagaactta
    29521 gagataagat tggatttgta ccacaaaaag cttttttatt tagtggaaca atagaagaaa
    29581 atataaggta tggaaaagaa gatgcaagta ttgaagaagt aaagcatgct gcaagtatag
    29641 ctcaagctga tgaatttatt tctgatatgg aagacaagta tgactcattt gtagcacaag
    29701 gtggaaataa tttgtcaggt ggtcaaaaac agagaatatc cattgcaaga gcgttagtta
    29761 gaaagcctga agtatatgtc tttgatgata gtttttctgc attagatttt aaaacagata
    29821 aaagacttag aaaagcgttg aaaaatgaaa tcaaagattc atcagctata attatagcac
    29881 aacgtataag tacaatcatg gatgctaatc agattatagt tttaaatgat ggaaaaattg
    29941 taggtattgg aaaacataaa gatttacttg aaaattgtga agtttataag caaattgcgg
    30001 attcacaact tagtaaagaa gaacttgcat aagatgaaga attaatctga tatttggagg
    30061 tgtatgatat gagtgaaaaa agaatagatg aacaagatat agaagtactt agttttgaag
    30121 atggcatgtc agcagaaaaa gctaaggatg caaaaaaaac tactaagaga ttattaaaat
    30181 atatggcaaa acaaaagttt aaaatattaa ttatttttat ttcagtattg ataagttcag
    30241 cgcttacagt tttagcacct atggttatgg gaaaagctat tgaccaacta tttaatggaa
    30301 taaaaactgc agtccaaaca ggtacaaaat tcagtgtgaa ttttagtact atggggggta
    30361 tagtatcaat acttctaggg ttgtacttaa ttagtgcagt atttatatat attcaacagt
    30421 atattatgtc aggagttgca caaaatctag ttcttagcat gagaaaagat ttaagtgata
    30481 aactcaataa acttcctctt aagtactatg attctcataa aaagggggaa acacttagta
    30541 tagtaacaaa tgatttagaa aaagtagcag atagtttaca agaaggatta atgcaactta
    30601 ttacagcagt agttacagta gttggttcta ttgtaatgat gataagtata agtgtaccac
    30661 ttacaatagt atcagctata acattacttg tttcattggg tattactgtt gtaattgcaa
    30721 gaaaatcaca agaaagattc tctgaaaatc aaaaggcact aggagagtta aatagcaata
    30781 tagaagagat atttactggt caaatagtgg ttaaagcatt tagtaaagaa aaggatacta
    30841 taagaaactt taaaatatta aatcaaaatc tttataatgc aagtagaaaa gcacagtttt
    30901 cgtcatatgc aatatctcca attattagat ttataaacct aattggctat gttattatag
    30961 cagttgtagg tgggatattt gcatcaactg gagcaatgac tcttggtagt atacaagcgt
    31021 ttattcaata tgtaaatcaa gcatcagagc ctactacaga aatatcttat attgtaaata
    31081 tgcttcaagc agcaatagca tctgcagaga gagtttttac agttatggac gaggtggaag
    31141 agataaagga tgaagaatct tcaaaggtta ttagcatgcc taaaggtaaa gtacaatttg
    31201 agcatgttaa atttggatat agtgatgatt ttattcttat gaaagatatt aatatcaatc
    31261 taaatgcagg agataagatt gcaattgttg gacctacagg agctggaaag acaactcttg
    31321 taaatttact tatgagattt tatgagatac aaggtggaag gattactatt gatggtgtta
    31381 atataaaaga tttaaagcgt ggagaactta gaactatgtt tggtatggtt cttcaagata
    31441 catggctatt taatggaagt ataaaagaaa atattgcata tagtaagtca gatgccacaa
    31501 tggatgaaat tgtaagagca gcaaaatcag caagagtaga ccattttata aggactttac
    31561 cacaagggta tgacactata ttgactgagg atgcttctaa tctatctcaa ggtcaaaagc
    31621 aacttctaac aattgccagg gcaatacttt cagacccatc agtattgatt cttgatgaag
    31681 caacatctag tgtagatact agaacagagg tagaaattca aaaggcaatg aataacttga
    31741 tgaagggaag aactagtttt gttattgcac atagattatc aactattcga gatgctgatt
    31801 taatattagt aatgaaagaa ggaactatta ttgaacaagg aagccataag gaacttattg
    31861 aaaagaaagg attctatgaa gaactttata acagtcaatt tacaagtgaa tatgatgaag
    31921 atgttgtatg attatcaagt tttaggcaac taaatgattg ataaatatta agttaaatat
    31981 agtaacatgt aaggttatat ggaataagag tatttctcta tttcatataa ccttttttat
    32041 ttatcaaaat aagataaaat ttcctttcta aattagattg aagaatgtat ttatcaatat
    32101 aaaagtaaaa taatcattat tattagtgaa gtaaatattg tttgccacat atatgccata
    32161 aagatgcact tattttacgg agatttaaca aagtagtgta aagtatactc taaatataaa
    32221 ataaatcaaa tagagttaaa taaattgaga ggtgatataa atagtgaata aaaaattgaa
    32281 ctatagattt gaaatgaagc acaaaataac tgaagcagat gtgttagctt taaaatcaag
    32341 actctatcca ataatgaaga aagatgaaaa tgcatctaaa gatggaaaat atcttattag
    32401 gagtttatat tttgatactc ctgaagataa agcacttttg gataaattaa atggcgttgc
    32461 aataagagaa aagtttagga taagatttta taataatgac tgctcttata taaggttaga
    32521 gaaaaaaatc aaacattaca acatgacatc aaagctatca actaatttaa ccaaaaaaga
    32581 agtaataaat atactaaata atgatattga atttctaaaa gagtcgacaa attgtttatt
    32641 aagagaattt tatttgaaat taaaatgtga aagattagag gcaaaaagca tagttgatta
    32701 caatagagaa gcttttattt atccagttgg aaatgtaaga gttacaatcg atagtgatat
    32761 taaaacttct gtaaattcgg tagatttatt taataaagat ttgccaacta tttcggtaat
    32821 tgatgagaat atgactgtat tggaggttaa atatgatgaa tttataccag attttataaa
    32881 ggatttaatt caaattaata agactacctc tacagcagtt tcaaagtatg cttcaagtag
    32941 attatatatt taagaataaa ggctatgatt tatataaaga tatacataaa taaggttaaa
    33001 aaacaaataa gttaaaaata aataaaataa aaaataaata agttaaaaat aggaaggatg
    33061 attttaatta tgacatttaa tgatattttt aaatcaagtt ttatagaaaa cgtatcaggt
    33121 ttttcatttg tggacagtgc tttagcacta ggttcagctt ttttagtagg tttgtttata
    33181 tatatggtct ataaaaagac ttatatggga attatgtatt ctcgaccatt caatgtgtcg
    33241 cttgtagcac ttacaatgct tacaactttt gtaatattgg cggttacttc aaatgtagtt
    33301 ttatccttag gtatggttgg tgctttatct atagtaagat ttagaacagc aattaaagac
    33361 ccaatggatt tagtatttct attttggtca ttaggttcag gtatagtttt aggagcaggt
    33421 ttgattccct tagctgtcat gggttcaata attatgggat tgattctaat attcttttca
    33481 aataaaacaa tatctgaaac tccatatata ttgatggtaa attgtaataa tgaagattca
    33541 gaagatatag caactgataa aattaaaaaa gtatttacta aatatcaaat aaaatcaaaa
    33601 agtgttacac cagaaaaagg aatagaatta gtgtttgaag taagaatgaa agatggagag
    33661 acaagtctta taaatgactt gagtcaagta gatggagtta ctaatgcagt tcttgtaagt
    33721 tataatggtg actatgtagc atagagagga gtaatctttt atgaaagata aaaaatttac
    33781 ccttcttatc tcgattatga ttatattttt atgtgctgta gttggagttt atagtacatc
    33841 tagcaacaaa agtgctgatt tatatagtga tgtatatatt gaaaaatatt ttaacagaga
    33901 caaggttatg gaagttaata tagagataga tgaaagtgac ttgaaggata tgaatgaaaa
    33961 tgctataaaa gaagaattta aggttgcaaa agtaactgta gatggagata catatggaaa
    34021 cgtaggtata agaactaaag gaaattcaag tcttatatct gtagcaaata gtgatagtga
    34081 tagatacagc tataagatta attttgataa gtataatact agtcaaagta tggaagggct
    34141 tactcaatta aatcttaata actgttactc tgacccatct tatatgagag agtttttaac
    34201 atatagtatt tgcgaggaaa tgggattagc gactccagaa tttgcatatg ctaaagtctc
    34261 tataaatggc gaatatcatg gtttgtattt ggcagtagaa ggattaaaag agtcttatct
    34321 tgaaaataat tttggtaatg taactggaga cttatataag tcagatgaag gaagctcgtt
    34381 gcaatataaa ggagatgatc cagaaagtta ctcaaactta atcgctgaaa gtgataaaaa
    34441 gacagctgat tggtctaaaa tcacaaaact attaaaatct ttggatacag gtgaagatat
    34501 tgaaaaatat cttgatgtag attctgtcct taaaaatata gcaataaata cagctttatt
    34561 aaaccttgat agctatcaag ggagttttgc ccataactat tatttatatg agcaagatgg
    34621 agtattttct atgttaccat gggattttaa tatgtcattt ggtggattta gtggttttgg
    34681 tggaggtagt caatctatag caattgatga acctacgaca ggtaatttag aagacagacc
    34741 tctcatatcc tcgttattaa aaaatgagac atacaaaaca aaataccata aatatctgga
    34801 agagatagta acaaaatacc tagattcaga ctatttagag aatatgacaa caaaattgca
    34861 tgacatgata gcatcatatg taaaagaaga cccaacagca ttttatactt atgaagaatt
    34921 tgaaaaaaat ataacatctt caattgaaga ttctagtgat aataagggat ttggtaataa
    34981 agggtttgac aacaataact ctaataacag tgattctaat aataattcta atagtgaaaa
    35041 taagcgctct ggaaatcaaa gtgatgaaaa agaagttaat gctgaattaa catcaagcgt
    35101 agtcaaagct aatacagata atgaaactaa aaataaaact acaaatgata gtgaaagtaa
    35161 gaataataca gataaagata aaagtggaaa tgataataat caaaagctag aaggtcctat
    35221 gggtaaagga ggtaagtcaa tgccaggggt tttggaagtt gcagaagata tgagtaaaac
    35281 tataaaatct caattaagtg gagaaacttc ttcgacaaag caaaactctg gtgatgaaag
    35341 ttcaagtgga attaaaggta gtgaaaagtt tgatgaggat atgagtggta tgccagaacc
    35401 acctgaggga atggatggta aaatgccacc aggaatgggt aatatggata agggagatat
    35461 gaatggtaaa aatggcaata tgaatatgga tagaaatcaa gataatccaa gagaagctgg
    35521 aggttttggc aatagaggag gaggctctgt gagtaaaaca acaacatact tcaaattaat
    35581 tttaggtgga gcttcaatga taataatgtc gattatgtta gttggtgtat caagggtaaa
    35641 gagaagaaga tttataaagt caaaataaga ttaaaattga atataaataa ttttaatact
    35701 gttaagtcta aatatttact tagcagtatt tttttagaaa ataatgtatt tggttaataa
    35761 tataaggtaa taaattgata aaaattaaat acagcatatt aataatacat aataaagtaa
    35821 taaaaaaact gtttaagtat aataacttat atttagacag tttttttcat ttataaataa
    35881 tataaatgaa attgaaaaca tttgaaggat agaaattggt tggataaatg tataaattac
    35941 ttcaccaaaa aataatgaca aaaactgtaa ctaagttgta ataaaatggt agttatttag
    36001 cataaaataa tgtatcatag aagtatatta taaaattaat aaaataagat ttttgtaaga
    36061 aaactgctgg cttttgatat ttagagtgag tagttaaatt atgtttagga ggggtagaat
    36121 ttgttgcaaa aagtattatg gaaattttta aagctagtag tcggtttatt tatatgttca
    36181 gttggtatag tattgacgat aaattgtaac cttggattat ctccctggga tgtatttcat
    36241 caaggacttt caaatcatat aggaataact ataggaactg cttctataat tgtaggctct
    36301 atagtagtta ttgctgatgt cgttcttgga gaaaatgtag gatggggaac tgtttttaac
    36361 atgctactaa ttggattttt tatggattta cttttatata gtaatttaat tccagaggct
    36421 gatagtttat ttgtaggaat aattatgttg atattaggat tggttctttt gtctgttgga
    36481 atggtatttt atatgggaag tggtttagga agtggaccta gagatggttt gatggttgca
    36541 attcaaaaga aaacaggaaa atcacttaaa ttaataagag gtactataga agttggagca
    36601 ttaatagtag gatttttatt aggtggaaaa gttggtatag gaactataat aagtgcattt
    36661 ggattggggt atttcacaca aatggtattt ggcttattta aattagattg tagtaagatt
    36721 aaacatagat ttatagtaga tgatataaaa tttatcaaag tgtatatgag tggtgagaaa
    36781 aaagctgctg ttcaaaatat agttaagtaa tttaagaata aattcaacga aatgttaagt
    36841 attttaaatt aacattttgt ttagatagat aaattgaata ttaaaagaat attaaaatat
    36901 gaggattgct agaacactat aaaatataga tttctagcaa ttttttattt gttcaatatt
    36961 tttttagtac tagaagtctt attctctatt taatgaatga attaatttat aattagagat
    37021 actaaacttt atagatatta atataaacac ctttaaactg tgtattttcc aaagccttta
    37081 aaatgcttgt atttattcta aataaaaaaa taaaaaaatg tcaaaaaaac tcttgtatta
    37141 tatccaaatt ttatatataa ttacctatgt tgtttagaaa ttttaaactc aatgtttagt
    37201 aaacaaaatg tttagtaaaa taaaacagaa actaggaggt gcaacatgga tataggtgaa
    37261 aagataaaaa gattaagaac agaaaaacaa ctgacccagg aagagttagc taatagatgt
    37321 gaattgtcaa agggatttat ttctcaatta gaaaataact taacatcacc atccatagct
    37381 acccttatag atatacttga aatactaggt acaaatttaa gggaattttt taatgaaata
    37441 gatgatgaaa gaatcagttt tacaaaagaa gatatgtttg aaactgaaga tgaagattta
    37501 aaatataaat taaagtggtt aattccaaat tcacaaaaaa atgaaatgga gccaataata
    37561 ataacattgt atccaggagg acagtataaa gaagaaaagc cacatgaagg tgaagaattt
    37621 ggatatgtat tagcaggttc aatatatgtc catataggcg aaaagaaaaa taaagtaaaa
    37681 aaaggagaga gtttttactt tagaccgaaa gcaaatcact atatatcaaa cgagggcaaa
    37741 actacagcaa aagtgatttg ggtaagtacg ccaccatcat tttagaatag aggtgtttaa
    37801 taattggtag aaaatataat agaattaagg gatatatcta aacattatga agaacttact
    37861 gttttagata atttcaattt agatataaag aaaaatgagt ttctaacatt acttggacca
    37921 agtggatgtg gaaaaactac aacattaaaa ataatagcag gatttgagta tgctgatgat
    37981 ggtaaagttt tatttgaagg taaggagata aataatttgc caccatatga aagacaagta
    38041 aacacagtat ttcaaaaata tgctttattt ccacacatgg atgtttatga aaatgtagct
    38101 tttggattaa aaattaaaaa gactccaaaa gatgtaatag atgctaaagt aaaagaggtt
    38161 ttgaaacttg ttgcactaga aggttttgaa agacgacaag ttgaatcatt gagtggagga
    38221 caacaacaaa gaattgcaat agcaagagct ctagttaatg aaccaaaggt attattacta
    38281 gatgaaccac ttggagcact tgatttaaag ttaagacaag agatgcaaat agaattaaag
    38341 agaatccaaa aaaaattagg aataacattt gtttttgtaa ctcatgacca agaagaggct
    38401 cttactatgt cagatactat tgttgtaatg tataagggca aaattcaaca aatgggaaca
    38461 ccacaagata tatacaatga acctaaaaat tcatttgtag ctaaatttat aggtgaaagt
    38521 aacatatttg atggaattat gatagaagat tataaagtta atttctgtaa tagagatttt
    38581 gagtgtgttg ataaaggatt cgaaaaaaac gaaaatatag aagtagtaat aagaccagaa
    38641 gatataaaaa tggtaaagcc agaagaagga atgttaaagg ggaaagttac atctacagtg
    38701 tttaaaggag tgcattacga gatagaattg aatgaaaatg gaagattgtg gatacttcat
    38761 aatactaaaa atgctgaggt tgggactgaa ctaggcatgg atatatatcc agaagatatc
    38821 catataatga gaaaagagaa taactaagat gaaaagaaaa tcttttttag catatccata
    38881 tgtagtttgg agtgcgatat ttgtaataat tcctttaata ttagtagtat tttttagttt
    38941 tacaaaagaa tcaggtggag gttatgcctt tacacttgag aattataaag aagtaattga
    39001 ccctatatat atgaaagttt ttgggcgttc tattctttta gcaggaggag ctacattaat
    39061 atgccttata gttggttatc cagtggcata tataatttca aaggctcgtg taagtagaag
    39121 aggttcatta atattattgt ttattcttcc tatgtggatg aactttcttt taagaacata
    39181 tgcatgggtt gctatattag gaaaaaatgg acttttaaat acatttttag gttggtttgg
    39241 aatacaacca ttagcaattt tgtatactaa ttttgctata cttttaggta tggtgtataa
    39301 ctttttacca tttatggttc ttccaatata tacagcacta tcaaagatgg ataatgattt
    39361 gataaacgct gctcatgact tgggtgcaaa taatatgaca gtttttagaa aaattatatt
    39421 tcctttgagt ttgccaggtg taatgtctgg aataactatg gtatttatgc cagcagtcac
    39481 tacttttgca atatcaagac tgttaggtgg aggtaaaata atgctggttg gagatttgat
    39541 tgaacaacag tttacagttg ttggagattg gaactttggt tcagctatat cgatatttat
    39601 gatgatagta atattaatat ctatgtctat aatgtcaaaa tttggagatg aatctgacaa
    39661 agaaggcggt gggttactat tttaaaatta aagaaaataa cttctaatat atatttagca
    39721 ttagtattct tatttttgta tgcacccata tttgctttgg tactattttc tttcaatgat
    39781 tcaaaatcaa tggcaagatg gaatggattt acatggaagt ggtatggtca attacttcaa
    39841 aatgagagta taatgagtgc tttatattat actattgtaa tagctatatt agcgtctgta
    39901 atatctacaa tagtaggaac tataagtgct ataggaatac ataagatgag aggtaagagt
    39961 aagaaattaa tactaaatgt aaactatctt cctattttaa atacagagat agttacagca
    40021 gtagctctta tgagtctgtt tgtatttgta aaaatggaat ttggatttac aacaatgcta
    40081 ttggcacata taatgttttg tttgccatac gttatacttt cagttttgcc aaaaatgaag
    40141 caattacctg ataatataga agatgcagct atggacttgg gagcaacacc tatatacgct
    40201 cttagaaaag taattttacc acaaataaaa ccaggtatag tttctggatt tttagttgca
    40261 tttactatgt caatagatga ctttataata agtttcttta atgctggaaa tggtgtaagc
    40321 aatctttcta tagaaatcta tggaatggct agaagaggta taaaacctga aataaatgca
    40381 ttatcaacaa taatgtttgc agtggttcta ggtttacttt tactagctaa taaaaaagaa
    40441 tctatagtaa gggggattaa ataatgatta agtttaaaaa agcaatatcc cttatagctg
    40501 taggtatgat gacagttact ttatttacag gatgtaataa agcaactgat tctacaaaag
    40561 ttttaaatgt atataatgtt ggagattata tagatgagtc tcttatagat aagtttgaaa
    40621 aagaaacagg aatagatgtt caatattcaa cttatgatac taatgaaatg atgtatcaaa
    40681 aagtaaagag tggtagtaca cactatgatt tagtatttcc ttcagactat atggtagaga
    40741 aaatgaaaaa tgaaggtctt ttggaaaaat tagattttaa gaatatacct aatatgaaat
    40801 atttagataa gagtttttta aatcctgtat acgacaaaac taatgaatat agtgttgcat
    40861 atatgtgggg aactcttgga atattgtaca ataagaaaga agttaaagac cctatggata
    40921 gctggaatat actttggaat ccaaagtata agggaaatat aatgatgttt gactctgtta
    40981 gagatactat agggataact cttaaaaaat taggatatag catgaatagt gtaaatccaa
    41041 aagaaataaa tgaagctaaa aatcttttaa tgaaacaaaa ggatttagta ttggcttatg
    41101 taaatgatga aggtaaggat agattgcttg gtggagaagt ggcaatgggt atgctgtatt
    41161 ctggagatgc agttacacta atggagcaaa atcctgacct tgattatgct atacctaaag
    41221 agggaactaa taaatgggta gatgctatgt gtgtacctac aacagcacaa aacaagaaag
    41281 aagctgagct gtttataaac tttttattag acccagagaa tgcaaaagta aatgctgagt
    41341 acattgggta ttcaacacct aatacaggag cactaaaatt attagaccca gaaataactc
    41401 agaatcctgt tgcatatcca tctaaagaag tacttgataa atgtgaaact tttgaagata
    41461 ttggtgaaaa tataaaatta tatgacaagg cttggataga gttaaaatca aaatagggtc
    41521 ttgttgtttt aagtaaattt acttaataaa tacttaataa aactgttctt ttttagaaaa
    41581 tttattttct aattattgaa cagttttatt ttttgtttaa gttatattca tatgcaattt
    41641 aaatagttta ttaataaatg aaaacttatt tgtataatga ctttaataat aattagaaaa
    41701 aatgggcaac ttaatagaat aattggaggt gtaaatttat aaaaaatgaa taggataaat
    41761 aaaaaaatag atttattaat attggctttg ctcgttttta tatttttaat tgttggaata
    41821 gtaatattat cgattaggac agaaaataaa ttgaatgtgt tcataatgtt agcaataatt
    41881 ttttttataa taatgttaac ttatttatca aattcagtag tggggttgat aacaagttca
    41941 ataattatat ttatgtatac atcctatatt ttatataaca atattactca caatatggat
    42001 gttgaattta tatcatatat gtggataata gcaacaccag tatcttcaat tataatgggt
    42061 aatctgaaca aaagcataaa tgaattgcaa aatacaaata aaaaactatc tgaacagtat
    42121 aaggaattag tgactattga tagtgaaact ggacttagaa atttaaagat tttttataat
    42181 gatgtaaata tggagataag taaatctatt agacataata ctgatttttc tcttatgata
    42241 gtaaaattac catactacgg aaacctacag actatatttg gtgaaaacaa aactaataaa
    42301 atagtaaaat atattggttc aaatattata gaatgtacaa gaaatgaaga tataatttat
    42361 agtttacaaa aagatatgat agggatacta atgccaaata catctttgga aggttcaaaa
    42421 gttgttaaag acagaataaa gaaaaggata aaagaattaa acttagattt aaacaatagg
    42481 ggaaaatatg taaatataga tgtcaaaatt gcatttttac aatataagag tagttttgga
    42541 gatagtataa attttaagaa tatagttgaa gaggaacttc aatatgatgt ttaaaatatt
    42601 taataaaagt ctattaaaat tagtagttgt attatcaata gtactgacaa tatcaaattt
    42661 tagcttatct tcatatgcat cagaaattac ttccagcaca gataaggtat taatcatata
    42721 tgattctaag aaagaaacag cttataatag agatatttta aatattatga ggactctatt
    42781 gggaagattt agttcagata tagagctatt aaaattgagt aactatgatg gtgagataaa
    42841 taaaaattat tacagtcata tatttatttt aggaatcaat gaaaactcat ataataatga
    42901 taaaaacact aaaaacttga ttagttcctt aaattcatat aaaggaacta tatgttggtt
    42961 aggatatggt atagaaaatc ttttggaaca taaaaaatac aatttagact atgtaggaaa
    43021 aacaaacaat atagttagtg taaattatag aggtaaatca tataacttgg atgaacatta
    43081 tgtttttaac attgtagaat caaaagatac aagtaataaa gtaataggaa gtattaatga
    43141 taccttaaat aagtatccat atataataaa tgataaaaat cttttttatg tatcaaaatt
    43201 agatttagat ggagttttat tttatatatt ttgcgattca cttaatgata tttttaatat
    43261 taagacgttt gataaaggac gtatatttgt aagaatagaa gatgtacatg cttttagaga
    43321 accaaaaaat cttgtagaga tagcggatta tttaagtagt aagaatatac catttacgat
    43381 tgcattaata ccagcatatg taaatcctaa aaatcataaa gtaataactt tatcagaaag
    43441 ccctgaaatt gtcaaagcaa taaaatatat gcaagataaa ggtggaactg tgatattgca
    43501 tggatatact catcaataca agaaagaaga agtttctgga gaagggtatg aattttggga
    43561 tggtaaaaaa gatgaaccct taaaagaaaa tatgaagata tttgtcaaag atagagtttt
    43621 gaatggtctt agagtatgta tagagaatgg aatttatcct cttgcatttg aagcgccaca
    43681 ttatgcaatg gaatcagagg gatataaaga attaaaaaaa tatttttcta cttatatggg
    43741 gcaacatcaa aataatgata aaaaatttag cacaaataca tatccttata ttattaggga
    43801 tacagaggag tttaatatat ttatacccga aaatttagga tatatagacc cagaagataa
    43861 gtttactttt caaaatataa aagaaaattt aaataaatta tccattgtaa gaggttttag
    43921 tggagggttc ttctttcatt catatttaaa catagagtat ctaaaaaaca caatagaata
    43981 tttagaaaaa cagaatatag aatttatgaa tttaagagat tttaataatt gggtaaaagt
    44041 tgatgaaata caaataagaa ataatggaga tgaaattata gtcaactatg ataaggactt
    44101 agatgaacta actaaaagtg atactagatt taaatccata tccaatatta gtaaaattct
    44161 aatattcata gtatctataa gtgttttaat atttgtgatt atatttatat attttaagag
    44221 aatagataaa aagaaatttt taaaataaat gattttatat tagaatcaat attttaaaat
    44281 attttaggag aaatttatga gagatttaaa tgtatcagag tatttgttcg tatttagttt
    44341 gtttagtata tggtctttat tgcttataaa tattatttta gctatgggtg gatatatatt
    44401 ttattttaaa aactttgata aagaaataaa agaaattgat gaatatccta tgatatcaat
    44461 acttgttcct gctcataatg aagctaaagt gataggtaga actgtggagt ctttgctatt
    44521 actcaattat cctaagagta agatggaact tattgttata aatgacaatt cttctgataa
    44581 tagtaaagaa attttagaaa atataaagga taggtacaac aattataatt tcactataat
    44641 aaatacagat agtttaacag gtggaaaagg aaagtctaat gcattaaata ttggatatac
    44701 gatttcaaaa ggagatttta tagctgttta tgatgcagat aacacaccag ataaaaatgc
    44761 acttagatat ctagtacaaa ctatagtaat gaatgatgaa ttaggtgctg ttattgggaa
    44821 atttaggact cgaaataaaa ataaaaacct attaaccaaa tttataaata tagaaacatt
    44881 aagctttcaa tggatgtcac aagctggtag atggcaactt tttaatctat gtacaatacc
    44941 aggaactaac tttatattga ggagaagtat catagaagaa attggaggat gggatagtaa
    45001 agcaattgca gaagatacag aaataagttt taggatatat aaattgggat acaagataaa
    45061 gcttgtacca caatctataa catgggaaca agagccagaa actgttaagg tgtggataaa
    45121 gcaacgtact agatgggcaa aagggaatat atatgtatta atgaaatata taaaaaatat
    45181 atttaaacag ggaagaaata agattgtgtt tgatatagct tacttttttt ctgtatattt
    45241 tctattttta acatctgtaa ttatatccga tattttattt gttttaagta tatctaaact
    45301 agtagaaatt tctattccaa ttaacttttt tctaatatgg atactatctt atcttttatt
    45361 tattatagaa gtgagtataa gccttactat agaaaaaggt gaagcaacaa ttgaaaatat
    45421 attcatagtt gcaataatgt attttacata ctctcaacta tggctgtttg tagctataaa
    45481 aggaatgata gagtatctaa aagatataat ttttaaaaga gaggttaaat ggtataaaac
    45541 agaaagattt tgattcatgt acaggaggaa ttaacatgaa aaaatttatt atatcaatta
    45601 tatctttagt attgtttttt tcaaatatat cacttatata caaagtaaat gctgatgaga
    45661 ctaaagttaa aaattataag tttgaaagag atataactat tgatggtgtt ataggaagta
    45721 atagtacttt ttttgaagta aataaaaatt gggatattga agaagtttta ttgcatctta
    45781 attttagtaa gagccaaata ttaaatggag atgtttcaag tttaactgta ttgataaata
    45841 atgtaccaat aaaatctatt aaattaaatg ctaagacaaa ttataaaaat acattagaag
    45901 tattagttcc aaaagattat ataattcaag gatataatga aataaaaata aaaacataca
    45961 aaacaatatc tgataaaatt tgccaagatg attccaatac tgggaactgg atggttattc
    46021 acaaagaatc atatatatca attagatata agcagaaaaa ggtggaaaac tcaataaatg
    46081 aatatccgta tccatatgca gaaattgaaa ataatcataa attagataca actatagtag
    46141 taccagataa tatgactaga ggagagacta cagcagtgtt taatttggca tcagcatttg
    46201 gaaaaataac aaaaaatgat gatttaaagt tagatgttaa attatattca gaaatgaaaa
    46261 attggtcaga tgataatatt atatatattg gtaaacctga aaatacagca gaagaaattt
    46321 tggatatttt aagtataaag gaacaaaccc tattatcttc aaactgtata atcaagcaag
    46381 tagattcacc atacaataag aataagaaga tgatggtggt aattggtagt aatgaagatg
    46441 atttaataaa ggcatcaaat ttattaatag aaaatagact ttctaaccaa gttttatcat
    46501 catcagtact tgtaaacaaa gagactaata taaaaattaa tagggaacaa aaattaaatt
    46561 taggtcattt aacattaaaa gacttaggat attctgactt tttgttggaa ggagcattta
    46621 atcaacaagc tttgtttgat gtcaaaatac caacgggtaa ggtattagat gatggttcaa
    46681 agattatctt aaatttaaga tattctgaca atttagattt tgaaaaatct ttagttacag
    46741 tgtctataaa tgatgttatt gtgggaagta aaaaattgga taggtcacat tctaataatg
    46801 ataagttaga attaaaaata cctaaagata tagataataa aaactattat caagttaaat
    46861 tgacatttaa tttgagtata aaaaattcga attgtgttac tagagagagt aataatcctt
    46921 gggcatatgt atcaaataat tcttatttgg cgttatctac gaaagaaaat gaaaccttat
    46981 cttttgaaaa ttatccgtat ccatttgtta gagatgatga atttaatgat ttaactgtga
    47041 taatgccaga ttattcaggg tcacaagcta tgacttggat gtttagatta ggtgttactt
    47101 taggtgcgaa tataaattca cataatggaa atataaatgt aattagagga aaagaattta
    47161 gtgacaagta taaggatact aatattgtag tttttggagt gccacataat aattctgtta
    47221 taaagatgtt aaataataat ttaaatataa agttcgataa aaattactcc aattttatat
    47281 caaatgataa gataagcttt attgatgatt atggaaaaaa tatttctacc attcaattaa
    47341 taaaatcacc atataataat caaaaaaata tcatggtaat tagttcaatg aatgaaaaaa
    47401 acttatattt aggaatggat tatttgttga ataaaagtaa agttaatgat ttaaaaggag
    47461 atacattgat aattgatgaa tacggagagg tagaagattt agcttataat ttaaaatcta
    47521 agaaagaagt aaaagattca agttggaata tgagtataaa taagacaact aaagtatttt
    47581 taatgatttc ttttattact at
//
LOCUS       DAEMHZ010000012        46909 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000012 DAEMHZ010000000
VERSION     DAEMHZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.012"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(472..1722)
                     /locus_tag="KPK72_000619"
     CDS             complement(472..1722)
                     /locus_tag="KPK72_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG2487863.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1754..2695)
                     /locus_tag="KPK72_000620"
     CDS             complement(1754..2695)
                     /locus_tag="KPK72_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG2487864.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2935..4011)
                     /locus_tag="KPK72_000621"
     CDS             complement(2935..4011)
                     /locus_tag="KPK72_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2487865.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLSMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4384..4899)
                     /locus_tag="KPK72_000622"
     CDS             complement(4384..4899)
                     /locus_tag="KPK72_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG2487866.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5276..6346)
                     /gene="leuB"
                     /locus_tag="KPK72_000623"
     CDS             complement(5276..6346)
                     /gene="leuB"
                     /locus_tag="KPK72_000623"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG2487867.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6353..6844)
                     /gene="leuD"
                     /locus_tag="KPK72_000624"
     CDS             complement(6353..6844)
                     /gene="leuD"
                     /locus_tag="KPK72_000624"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG2487868.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6859..8136)
                     /gene="leuC"
                     /locus_tag="KPK72_000625"
     CDS             complement(6859..8136)
                     /gene="leuC"
                     /locus_tag="KPK72_000625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG2487869.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIKDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(8093..9835)
                     /gene="leuA"
                     /locus_tag="KPK72_000626"
     CDS             complement(8093..9835)
                     /gene="leuA"
                     /locus_tag="KPK72_000626"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG2487870.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9959..10205)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10353..10799)
                     /locus_tag="KPK72_000627"
     CDS             complement(10353..10799)
                     /locus_tag="KPK72_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487871.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10820..11539)
                     /locus_tag="KPK72_000628"
     CDS             complement(10820..11539)
                     /locus_tag="KPK72_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2487872.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSYMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11657..12931)
                     /locus_tag="KPK72_000629"
     CDS             complement(11657..12931)
                     /locus_tag="KPK72_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG2487873.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(13131..14498)
                     /locus_tag="KPK72_000630"
     CDS             complement(13131..14498)
                     /locus_tag="KPK72_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2487874.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14748..15212)
                     /locus_tag="KPK72_000631"
     CDS             complement(14748..15212)
                     /locus_tag="KPK72_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG2487875.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(15217..15846)
                     /locus_tag="KPK72_000632"
     CDS             complement(15217..15846)
                     /locus_tag="KPK72_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG2487876.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15880..16767)
                     /locus_tag="KPK72_000633"
     CDS             complement(15880..16767)
                     /locus_tag="KPK72_000633"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG2487877.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16796..18034)
                     /locus_tag="KPK72_000634"
     CDS             complement(16796..18034)
                     /locus_tag="KPK72_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487878.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKVAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18418..19560)
                     /locus_tag="KPK72_000635"
     CDS             complement(18418..19560)
                     /locus_tag="KPK72_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2487879.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19701..20567)
                     /locus_tag="KPK72_000636"
     CDS             complement(19701..20567)
                     /locus_tag="KPK72_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2487880.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20901..22448)
                     /locus_tag="KPK72_000637"
     CDS             complement(20901..22448)
                     /locus_tag="KPK72_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2487881.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(22441..23577)
                     /locus_tag="KPK72_000638"
     CDS             complement(22441..23577)
                     /locus_tag="KPK72_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2487882.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(23840..24937)
                     /gene="dinB"
                     /locus_tag="KPK72_000639"
     CDS             complement(23840..24937)
                     /gene="dinB"
                     /locus_tag="KPK72_000639"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG2487883.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLIK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(25327..26832)
                     /locus_tag="KPK72_000640"
     CDS             complement(25327..26832)
                     /locus_tag="KPK72_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG2487884.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(27094..27444)
                     /locus_tag="KPK72_000641"
     CDS             complement(27094..27444)
                     /locus_tag="KPK72_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487885.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(27667..27858)
                     /locus_tag="KPK72_000642"
     CDS             complement(27667..27858)
                     /locus_tag="KPK72_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487886.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(27976..28890)
                     /locus_tag="KPK72_000643"
     CDS             complement(27976..28890)
                     /locus_tag="KPK72_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2487887.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(29035..30531)
                     /locus_tag="KPK72_000644"
     CDS             complement(29035..30531)
                     /locus_tag="KPK72_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2487888.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(30811..32301)
                     /locus_tag="KPK72_000645"
     CDS             complement(30811..32301)
                     /locus_tag="KPK72_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2487889.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            32768..32968
                     /locus_tag="KPK72_000646"
     CDS             32768..32968
                     /locus_tag="KPK72_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2487890.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(33148..34026)
                     /gene="speB"
                     /locus_tag="KPK72_000647"
     CDS             complement(33148..34026)
                     /gene="speB"
                     /locus_tag="KPK72_000647"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG2487891.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNILNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(34016..34867)
                     /gene="speE"
                     /locus_tag="KPK72_000648"
     CDS             complement(34016..34867)
                     /gene="speE"
                     /locus_tag="KPK72_000648"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG2487892.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(34897..35310)
                     /gene="speD"
                     /locus_tag="KPK72_000649"
     CDS             complement(34897..35310)
                     /gene="speD"
                     /locus_tag="KPK72_000649"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG2487893.1"
                     /translation="MKFETLGRHILVEYYNCDENILKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(35397..36872)
                     /locus_tag="KPK72_000650"
     CDS             complement(35397..36872)
                     /locus_tag="KPK72_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2487894.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(37510..38163)
                     /locus_tag="KPK72_000651"
     CDS             complement(37510..38163)
                     /locus_tag="KPK72_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487895.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPMEEMKKEKLL"
     gene            complement(38408..40273)
                     /locus_tag="KPK72_000652"
     CDS             complement(38408..40273)
                     /locus_tag="KPK72_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG2487896.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELIICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRILILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(40278..42698)
                     /locus_tag="KPK72_000653"
     CDS             complement(40278..42698)
                     /locus_tag="KPK72_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG2487897.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(42712..44154)
                     /gene="glgA"
                     /locus_tag="KPK72_000654"
     CDS             complement(42712..44154)
                     /gene="glgA"
                     /locus_tag="KPK72_000654"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG2487898.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(44183..45313)
                     /gene="glgD"
                     /locus_tag="KPK72_000655"
     CDS             complement(44183..45313)
                     /gene="glgD"
                     /locus_tag="KPK72_000655"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG2487899.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(45315..46475)
                     /locus_tag="KPK72_000656"
     CDS             complement(45315..46475)
                     /locus_tag="KPK72_000656"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG2487900.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT        15127 a         8423 c         4772 g        18587 t
ORIGIN      
        1 gattcagtta gcaaattaat tttgtataca taaaatttaa accatataaa aacatatact
       61 ttagattaat actcaaaact taagttacca gataataatt ctagtaaaat attataatat
      121 ccttttacta ttttattttt ataattataa taattatatt tataaaaata aaatataaat
      181 ttcaaaatca ctttttttat taactttttt ataattataa taaaaatata taacagtact
      241 attataaata cacatttaaa accttataaa tatacaaata caattatatg aattttcatc
      301 ctcaattata gcatagaata tgactatcta tatcctaaaa ttatccattt ctaaaaaaaa
      361 ggtaactatt attaagaata tcaacttata gaatttatat tccacatacg attttcaatc
      421 aaaaaaacaa gggctaaaaa gtgaattgtg cactttttag ccctttatta attattcatt
      481 tatttcatgt aagaatagac cacatcttac tttaggttca aaccatgttg acttagctgg
      541 cattatctta tttgcatctg ctactcttat taaatcttca atactagtag gatacattgc
      601 aaatgctact ttcatatctt cattaactct tttttctaat tcttcaagac ctctaattcc
      661 accaataaaa tcaattcttt tatctactct aggattatca attcctaaaa tagcatcaat
      721 aacatagtct tgtaatatag aaatgtctaa tgagtcaaca gggtcttcta tttctaataa
      781 atttttattt gctttcattt tataccactt atcacctagg tacattccga aagttccttt
      841 tttgtcaggt ttatattttt cttttccttc tatttcttct agttcaaaat tttcttttat
      901 tttatttata aattcttcct cgcttaaacc atttaagtct ttgataaccc tattataatc
      961 caaaaccatt aaattctcat ctggtgcaat cattgctatg aagtaattaa attcttcttt
     1021 gccagtataa tttggatttt tagctctcat ttctttggct acattttctg ctgaagctgt
     1081 cctatgatga ccatctgcaa tgtatagata atctaatttt tcaaatttat ctactaggtt
     1141 atctattata tcatcattat caatcttcca tactgtatgc tctatcttgt catctgttat
     1201 gaaatcatat aaaggttcat tattatccat aaaatcattg attatatcat ctatcaccct
     1261 ttgatgtttg taagttacaa gtatagttcc cgtattagca tcacagtatt ttatgtgttt
     1321 agttctatca atttcctttt ctggtcgagt gtactcatgt ttttttattg ttccattcaa
     1381 gctttcatca actgatatac atgctactat accaacttga gctctaccat ccatgatttg
     1441 tttatatata tatatacact ctttgtcatc ttttactaga atttcttgtt ttcgaaactc
     1501 atataaattt tctcgcgctt ttaaataaac tttttcatca tataaatcta tattttcatc
     1561 taaatcaatt tctgatttat ctatatgcaa aaatgaatat ggattccctt ctgcaattct
     1621 tctagcttct ttactattca taacatcgta aggaagtgct gctactttat ctactatata
     1681 tttatttggt ctaatcgcct taaatggttt aatcgttgcc attactaatt tcctcctaat
     1741 tatacaaaat attttataat gctaccccta ttagattgtt gaagtcaaga aagaaatctt
     1801 gaatcacatt tactatttcg tctcctattc tttcttgagc ttctactgta gatgccccaa
     1861 tatgaggtgt aggagaaact cttggatgat ttactaagtc taaatttaat gtaggttctt
     1921 gttcataaac atctatagca gctgctgata atttaccttc atctaatgct tttagtaaat
     1981 ctttttcatc aactaaaccg cctcttgcac agtttattaa ataagcccct ttcttcatct
     2041 tatttatttc tttttcagtt attactgccc ccttattctt atcaaatggt atgtgtaaag
     2101 ataagaaatc tgcattttct aaaacttctt ccatatcaca gaatttataa ttgttaaagc
     2161 cttgcgcctc acccattatg tcagtatata taacattcat acctaataat tctgctctct
     2221 ttgcaacttc ttttgcaatt cttccaaaac ctataagtcc aagtgtcttt ccattaattt
     2281 ctgtaccttt atactttttc ttctcccatt ttccatctct catagttaca tttgcagtat
     2341 ttatgaatct tgcaagaaca aataattgac ctattgtaag ctctgcaaca gatatactgc
     2401 ttgcatttgg agtattcata acttttatgc cattagcttg agcatattta acatctatat
     2461 tatccagacc tactccacct cttactatca acttcaactt tttaccctct aatgctttat
     2521 ctattacatc tttagtaact ttagtagctg aacgtactac aagtacatca acatctttaa
     2581 gtttatctcc taaaacatct ttttcataaa actgctctat cacattaaaa tctaactctc
     2641 tcaattttct cgctgcttct ttctctattc catcagtaac taatatatta tacataacaa
     2701 actcctccaa tttttattca cttaattttt ccaattttta ttttttaaag tattttcatc
     2761 aataaaaaac tttacttaat ttaactttct gtttatataa ttttcaaaat atttttttat
     2821 taaagtcata tcaaattttc ctattgtttg ttaaaaattt aatatgtaat ttttataaaa
     2881 tattatttat tatatattct aaatttatct taaaacatac atatttttac aatattattt
     2941 attaaaacct aatatatcat caatattatc taataattct tgtacatctt cgacagtata
     3001 gtcacccata tgtgaaattc taaaagtttt ttcttttaaa tctccatagc catttgctat
     3061 ttggaatcct cgttcttgta attttgaatt taaatttgat acactaatac cttgagtatt
     3121 ttctataaca gtaagtgtat tggataagtg attttcattg gtaaaaattt ggaagtgttt
     3181 tttagcccag tttctaacta agtttgccat gtcttcatgt ctgttgaatc tgttatctag
     3241 gccttcatct aatatattat ctaattggaa atctaaagcg aacatgtgag aaagagatgg
     3301 tgttgaaggg tattggtagt ttttcttgat taagtattca tacattgata acaaatcaag
     3361 atatactcct ctaaaaggta cttgcttagc tctatcgatt gctttttgag aaaatgtgca
     3421 aatagacatt cctggtggta atcccagtgc tttttgtact gatgttatac aaatatctat
     3481 tccaatctta tcaacctcga cttttattcc accggctgaa cttacagtat ctacacagta
     3541 gattacatct tcatactttt taacaacttc tccaatttct tctattggat ttcttattcc
     3601 agttgaagtt tcattgtgag ttacagttat taaatcgtat tctccggtag aaagcacctt
     3661 atcaaccatt tctggcgttg ttgcctctcc taattcaact ttaaatatat ccgctgggac
     3721 attattagcc actgccattt tgtaccatct atcaccaaag gatccacagg aaaatactgc
     3781 tgcctttttg gatgtacagg aacgaatgga gccttccatt aaaccagttc cggatgacgt
     3841 ggataaaagt atcgtgttgt ttgtatagaa tagtttttgc atcttttcac ttatactctt
     3901 ttggagcata gatgcatctt ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa
     3961 gacttcttct ttaacatcaa taggaccagg aataaataat ttcttactca ttataattcc
     4021 ccccttttta gtttgtaata tggatagcgt ttttactgtc cttattttta ataactataa
     4081 tatcaagctg aattaactat gtcaacataa aatttatttt ttatatataa tttattttat
     4141 atattttatg aaaacattca ttttctacct tttcataagt ctgaattttc tgaattattc
     4201 tctcaaaaaa aatatacttt ttatctatat aaattaattt ttttagtatg taaatttgac
     4261 gctattacga taaatctttt tagtataact aatttatttt tatcttgtat ttttattgta
     4321 tttttaaatt ttattttttc taaatataaa atttatgttt taaaatgtaa aatttttatt
     4381 taatcaaagt tcaatatatt ccactttttc tttcgattct tcaaaaaacc attcttcatt
     4441 agaaaaatca ccttctgcaa tttgtccaat aggcattttc atatactttc cagttgctgt
     4501 cactgctata tctccatttg gtagaattat ttctcctgta ccttcaaata attttctgtt
     4561 ttctttagtt attcttccaa caacttttat agtagcatct gttggtactg gtttcttata
     4621 tttcaactct aaagatactg ttactcccca tatttggtca tcatttatac ttacagctct
     4681 tccaatagtc tcatccaata ttgcagcaga catacctcca tgaactctcc caggataact
     4741 ttgatgccaa tcctttgtat tacaaattga tacaagctct ccattttcaa gttcataaaa
     4801 atctgcttta agtcctagct cattttgtgt tccacaaact aaacaagatt tactactatt
     4861 ttgcttcttc aaaactttat actttgccaa aaaatccatc tcctaaccga cattagtagt
     4921 cgcttaatat taaataattc taaaatatat tatataatat taataaaatc cctgcaaact
     4981 tctaataata aaaataggct acttcaatat gataagttta atttaaagta ttattttcca
     5041 aaaaataact ttatcaaaat agaaactcaa agtagatata agcctctttt tcgttattaa
     5101 aatagtaaaa atatatttta cttaatataa aaaagagtgt ctcattaact aataaagttt
     5161 attttgagac acccctcata aaacaaatta tactacaata gatttttact aattctacaa
     5221 gttatatatt caaattatat tattcaaatt atattattca aattaaacta taattctaaa
     5281 tttcatttac tattaattcg cccattgctc ttgttcctac aacatttccc acaccattat
     5341 agatatcaat agttctataa cctttattga gtacactttg tacagcatct tcaatacact
     5401 tagcttcttc ttctaagtta aaactatgtc ttaacatcat gcttacagat agaatagtcg
     5461 caattggatt tacaatatct tttccagcta tatcaggtgc acttccatgt attggttcat
     5521 acataccaaa actatcttct ctaagtgatg ctgatggtaa catacctata gaacccgtaa
     5581 tcatagatgc ttcatcagat attatatctc caaatatatt attagtcact attacatcaa
     5641 attggctagg gtcttttaca acttgcatag cagcattatc aacatacata tgtgacagtt
     5701 ctacatcttc aaactcttta gctaagtctt ccattatact tctccaaagt cttgaactct
     5761 caagcacatt tgccttatct acacttataa gttttttatt tctatttctg gctatttcaa
     5821 aagctctctt accaattctt cttatttcat tttcgttata tatttctaca tcataagcat
     5881 tttcgatacc atcaatgatt tttcttccac gttctccaaa ataaatgccc cctgttaatt
     5941 cccttacaac aacaaaatca atgcctttct caactatatc cgttctaagt ggtgaagctt
     6001 cttttatact ttcatacatt gtagctggtc ttaaatttgc aaataaaccc aatgactctc
     6061 ttaattttaa aagaccagac tctggtcttt tatctccctt acatttatcc cactttggtc
     6121 ctccaacaga accaagcaat acagcttcgt tttttcgaca agtttcaaca gttttattag
     6181 gtagaggttc tccagtttca tcaatcgcgc atcctccagc taatacatat tcacaatcga
     6241 atctatgatt aaatttacaa caaattttat ttagaacctt tataccttca tctataattt
     6301 ccggtccaac accatcacct tttatgactg ctatattaca attcatactc cactaccccc
     6361 tatttgcttt tattgagtta attaagcctt catttaatat aatattttgc ataaactcag
     6421 gaaatggttc tgctttatat tcttttcctt ttgttatatt ttttatcaca cctgtagaaa
     6481 aatctacttc tatattatcc ccttcatcta tgttattggc agcttcttca cattcaagta
     6541 taggaagccc tatatttata gagtttctaa aaaatattct agcaaaagta gaagcaatca
     6601 cacaggatac tccactttct tttattacaa ttggtgcatg ttctcttgat gaaccacaac
     6661 caaagttttt agttgcaact ataatatctc ctttttttac cttagagata aatttatcat
     6721 caatatcctc catacaatgc gttgctagtt ctttataatc agctatattt aaatatctag
     6781 ctggtattat tacatctgta tctatattat caccgaattt aaaaacactt ccattggcaa
     6841 tcataaattt tacctccctt aaattatttc tgatggtttt gatatttttc cagttatagc
     6901 tgatgctgct gctacagctg ggcttgcaag atacacctct gaatcaacat gccccattct
     6961 tcctacgaaa ttcctatttg ttgttgaaat ggatttttct cctgctgcaa gtatacccat
     7021 atgtccccct agacatggac cacaagttgg tgtagaaact gctgctcctg cctctataaa
     7081 agtggtaata tacccttttt ctatagcttc aagatatact ttttgagttc caggaaatat
     7141 cataactcta actccatctg ctactttttt gcctttcatt atctcagcta ctacttcaag
     7201 gtctgatatt cttccatttg tacaagagcc tattacaact tggtctatct ttatatcctt
     7261 atctatctca tctactgttc tagtattttc aggaagatga ggaaaggcta ctgtttcttt
     7321 tagttcagct aaatctattt cgtatacttc atcatagact gcatcttcat ctgcttcata
     7381 tatatttaca tcttttgtta tagttgaaca cttatgtgaa tttatatatt ccatagtctt
     7441 atcgtctact ggaaaaattc cattttttgc tcctgcttct attgccatat tacaaatagt
     7501 aaatctatca tccatagata agtattctac tccatctcca caaaattcca ttgacttgta
     7561 aagtgctcca tcaacaccta tctcccctat tatatgaagt attatatctt taccacttat
     7621 ccatttattt ggtttatttt tcaaaacaaa ttttattgct ccaggaacct taaaccaaca
     7681 ttttcctgta gccatacctg ctcccatatc agttgaacct acaccagtag aaaatgctcc
     7741 taatgctcca tatgtgcaag tatgagagtc agcacctata attacatctc cagcagttac
     7801 tatacctttt tcaggaagaa gaacatgctc tattcccata tctcctacat caaaaaaatt
     7861 tacaactcct tttgattttg cgaaacttct tgtaaattta cattgttctg cactctttat
     7921 atctttatta ggtgtaaagt ggtctaaaac catcactact ttagtcttat cataaacatc
     7981 tttaattcct aatttttcaa attctctaat ggctactggt gatgttatat cattacctaa
     8041 aactacatct aaatctgctt cgattaattg ccctttctta acttcattta atttagcatg
     8101 tttggctaat attttctgcg tcatagtcat ccccattttt tatttcctcc tcaatataac
     8161 ttttatttat tgcattcata agtgcattaa tagctgctgt atttatgttt tccgatatac
     8221 ttacaccaaa atagacttca ttattgtgct ctattcctac ataagctacc cctcttgaat
     8281 gtgacccttc ctctaatgca tgttcccagt aatatctaaa tttataatct ttcatgccac
     8341 attgctttag tgcattatta aatgaatcta caggtccatt tcctatacca acaatccttt
     8401 gttcatttcc catataactt attcttgcag tcatctcaat catgttagta tcattagaat
     8461 catcattttc atagtttaat tcatccattg attttatttt atacttctta actttataag
     8521 ggctctcaac ttcaatatat tctttcttaa acaaattaaa tattgcttca ctactaagtt
     8581 cttccccaat tctatcagat tccattttga ctacatttcc aaaatcagaa tgcatattct
     8641 taggtatcat aaatccataa tctgtttcca ttatataaac agctcctcct tttccagatt
     8701 ggctatttat ccttataatt tctttgtatt ctcttccaat atcatgaggg tctattggta
     8761 aatatggaac ttcccagtaa tcattatctc tgtttttcat tgctatcatg ccttttctaa
     8821 tagcatcttg atgtgaacct gaaaaagcac aatgtacaag attacctgca tatggatgtc
     8881 tatcatgtac cataagtttt gtacagtctt catatatacc tattatctta tctatattgc
     8941 taaaatctag ctctgggtct attccttgtg tataaagatt aagtcccaca tttactatat
     9001 ccatattccc agttctttca ccatttccaa atagtgttcc ttcaagtcta tcggcaccag
     9061 ccaataatcc aagttcactt gcagccgtac aagttcctct atcattgtga gtatgcaaac
     9121 ttagtataat agaatcccta caacttatat ttttacaaaa ccattctatt tggtctgcat
     9181 atacgtttgg tgttgccatt tccactgttg atggaagatt tattattact tttttttgag
     9241 gagttggttt ccaaacatct atcactgcct cacatatttc aagtgcataa tccatctctg
     9301 ttcctgtaaa actctctggt gaatattcaa aagtaaattc tgtttccgga tattttttta
     9361 cttcttcatt aaataatttt gctccttcaa ctgctatatc tattatttct tgtttactca
     9421 tattaaaaac cacatctctt tgaagaacag atgtagagtt atataaatgt ataattgctt
     9481 ttttacatcc ccttattgat tcaaatgttt tttctataag atgagcccta gattgtgtaa
     9541 gtacttgtat agttacatca tctggaatca tattctcatc tattaatttt cttaaaaatg
     9601 tatactctgt atttgatgct gatggaaatc ctacctctat ttctttaaat cctgtatcaa
     9661 taagcatttt gaacattctg actttttcat ttacactcat aggtgttggc agtgactgat
     9721 ttccgtctct taaatcgaca ctacaccaaa ttggagcctt aaatatttca ttatctggcc
     9781 atttacgatt tactatttgc attttatcat attttttata ctttccacac ttcatgttat
     9841 ttcccccttt ttctgttttt tgtattcagt atacctcata tcgacgcaat cctgcacaat
     9901 tcataaggat aaatgtcaat tttatcatta gaataaaaaa aacctcatct ctaataaaat
     9961 tagagacgag gttttgctcg cggtaccact ctatttagct ttctaaaaag ctcccttagt
    10021 aatagccaat acaataatta taatgactat atttcctata acgtggaatt actccgatta
    10081 ttctaagatt taaatcttag aaaacagctc ctagacaagt tcagaactat caactactaa
    10141 atcacaccat cctttagctc tctgaaagtt tcattgttct tactacttct attcatcact
    10201 tttttaatca tttaaatttt ctaaatattt tgatttaata gttattatat acacattaaa
    10261 atgtattgtc aacacaaaaa ataattatct caatttttta tttttgataa gcaattctat
    10321 aaaacatgct aactattgaa aacagtatga tattatgcct taaacttttt catcttttca
    10381 tctatgatat tcaataattt tttttcatct tccaaactca atgcccttaa tctaatttta
    10441 taattagatt cgtcagtgta tatatttaat atatttttta ctacttctat tttttctatt
    10501 atcttataat taaacttttg actttgttta gattttatac tgaatataca ttgattattt
    10561 attaatattg aatgattatc gagagtaata tatacttttc cctcatattt atttgaaaaa
    10621 tcacgacctt gtttaaatag aaaaactgct gttattatac ataaaatata taatattgtc
    10681 gttgaatata taaaaattgt agctaaaatt ataagtgcta aaaccatata tatagtcatt
    10741 ccttttttaa aataaccatt gtcataataa ttcatataga taaacttatt tttactcaaa
    10801 ttttttcact cccaagattc taaaaattta tatcaaaaaa ttctataatt tcatcttcat
    10861 gataatttat gactaattca tctggaaaat ctactgcttc taaaactttt tctgcatttt
    10921 caaattctcc tatagaataa catatatgag agtcagtacc aagaattact cttgcaccat
    10981 atgttttaca caatgtcaac atataactta cattttccca agtacccgtt ctatgagagt
    11041 ttgaacttaa tgatgaatta tttatttcta aaaggatatt tttatcttta gcttttttta
    11101 ctataggttc ataatctaaa ggatacctac tgtcatctgg atgtccaatt atttttacct
    11161 taggtttatc cataactttt aaaatagcat ttgtattttc ttcttttgta ccagaagcta
    11221 tacaaggtct atgcaaactt gcgattatat aatccaaatg ttgcaacata tcttcttgaa
    11281 catctaaatt accattataa tccattatat ttccttctat tcccctaaga atctttacgc
    11341 cttgcacttc acgtggaagc aaatgcaaat tataaaaata gaatggatgt ggtcctcctg
    11401 gcatattagg tccatggtct gatattccta agtattttat attcttctta ctcgcctctt
    11461 cgacattttc cttgaaagta ctatgtgcat gtccacttat tagagtgtga caatgtacat
    11521 ccataataga ctttttcatt aaatttcctc ctaatatttt acataaattc gtgaaaatag
    11581 ctaaagggct atttcaaaac taaagctgtc ataatagaca gtgtaacgtt ttaaaacagc
    11641 cccattttca attattttac acttctaaat ctttaaattt atctttaaat aattctccaa
    11701 tacttcctaa agattcctct acttctaggt attctgttat atcctcttct ggctctctag
    11761 ttgcttcttt tattgaaacc aacattctct tagtctttgg tttaaaatca agaatcttaa
    11821 ctttaacctc atctccaata ttaacaacat tagtaacttt agccactcta gtatcactca
    11881 attcagatat tggtaaatac gcatctacac cttctttaat ttgaacaaaa gccccttttt
    11941 ctattattct aagaacttta gcattgacaa catcatcaat ttgtacttct tttgctatta
    12001 aatcccaagg gtcattattt atgtctttta atgccaaggc aattttttta gtctctttat
    12061 ttacatccaa cacataaact tctacttctt gtccctctga aacaacatct tcaacacttt
    12121 caactcttgt ccaagctaaa ttatttatat gtgctaatcc ttcaatccca ccaatatcca
    12181 caaatgcacc ataaggcatt atctttgtca ccttaccatc tcttttttgt ccaacttcaa
    12241 gagaattaaa taagtcttct ttagctgctt tgatacgagc tttttcttct tctctttctt
    12301 gcttaattct tgctctttca gcttcttttt ctgccttaat ttgagctctt ctttcttctc
    12361 tctctttatt tatacgctct tgtaatacat ctctatgaga tgctacaagt ctattttttc
    12421 taacatccaa ttcttttata taaacttcta agttaagacc aacaaattta gatgtatctg
    12481 taataaattt tgtatcaagt tgagaaattg gtataaaaaa tgtatatgta ttgtaattag
    12541 caaatacacc tttatctata ttcttttcaa catttacagt tattattcta tgttcatcat
    12601 aagccttttg taactcggca aaatcatttc ttttgtcaag ttgtaattta gataaagtta
    12661 ttgtaccatc tttttgagat acttttgtta ttacagcagt tatttcatca cctatgtggt
    12721 atatatcttc tatatattga tttttttcta tgttcaattc acttataggt attattccat
    12781 caaacccata gtctaatcct aattgtactt catcatttct cactgcagtt atttttccag
    12841 ttgtcaattc tccaactttc actttgctga atacttgttc ttgctggtca agtaattctt
    12901 gcattgttaa atcattttcc attataaaca tcctttcctc ttattgatta tcatatctgt
    12961 aaatattata ccattaatat tgcaaatact tacatttttc ttactataat ttttaaaata
    13021 aattcaactt catatattta tacattatag aaaatatctt attaaattaa aatacaaatt
    13081 ctaattttta tctaataaaa tattttcata aaaaatttat attgtatatc ttattctttt
    13141 attgctttag gttttgatag aaaacattta taaaatataa agaaagaaat tgctgttata
    13201 gtagcggctg ttatatctgc aactggctct gctaaaatta ctgcaaaaag tttttcatta
    13261 aagaatgttg gaagtataaa tattaaaggt attaaaagta caatctttct aagtagagct
    13321 aaaaccaaag atattgttgc ttttccaagt gctaaaaatg tctgttgaca agctatttga
    13381 atcccaaaca taaaagcacc agcaaaatat atttttatac tccaactagt catactaagt
    13441 aactgcggat tcttattaaa tatactaaca aaaaatgcag gaaatatcat taataaaagc
    13501 cataaaatag tagaatatgt aaaacatgac aataaacaca acttaaatgt cttttttact
    13561 ctatcaagtt gacctgaacc ataattataa cttattattg gttgtgctcc ttgtgtaacc
    13621 cccatattag gaagcataag aatttgcatt atactgctca ttatggccat tgaacctact
    13681 gctaaatctc ctccatacat ttgtaattta ctatttaagg ctataagtac taaactttct
    13741 gtagattgca ttataaatgg agagattccc aagccaagca ctggtaagat gatacttgct
    13801 tttggtatca tatacttctt ttttatttta agtatacttt tctttccaaa gagaaacata
    13861 agtacccaaa tagcagaaac gccttgtgca attatagtag ctaatgcagc tccttttaca
    13921 cccatattaa agccaaatat aaaaatcggg tcaagtatta tgtttattaa agcacctata
    13981 gcaactgtca tcattccagt tgtagcaaac ccttgggtat ttataaaagt attcataccc
    14041 attgaaatct gaacaaacac tgtacctatt aaataaatat ttaaatagtc cattgaatat
    14101 ccaattgtag attcacttgc tccaaacatc caaagtaaag gctcttttcc aatcttaaaa
    14161 cctatagtca aaagtattgc taaaatcacc agtacagaaa aactgttact cattattttc
    14221 tctgcctcat cattgttttg ctcacccatt tttatagcag ctaatggacc tcctcccata
    14281 ccgattaaat aactacatgc agttattata attattattg gaaatgccac tccaacacca
    14341 gccatagcaa cttctccatt ttccattcta ccaataaaaa tcctatcgac tatgttgtat
    14401 aaaacattta caatttgtgc gataatagca ggtgtagcaa gtttaaataa taatttaccc
    14461 acactttcac tacctaagtt agtgtttgaa ttttccattt taaaacctcc tcaatccaat
    14521 aattaaaatg tatcactaaa attttattct atataaaaat ttcattttat tagcagacaa
    14581 atttagagta ctttccctac aagtaatact atactatatt tcttttgcca tagtaaacat
    14641 tttagaacta actgaagtta aatactttat ctcatttttt atttattatt aaaaattttt
    14701 cacatatact cataaaaaaa ctatccaagt atattactta gatagtttta tattatatgt
    14761 aaagaaagag ctgaaactat aacccctgat aacaaattgc caagaaatac tcccataaaa
    14821 gcatctttta ttctcatttt taatatggaa gctatagcag atgcagtcca tgttcctgta
    14881 gttggcagtg gtatacctac aaatagtatt ataccaatta tcgaaacact ttttaatttc
    14941 ttcattcttg agtttatttt tctatcaatt acatcagcaa tccctttaaa tagtttcttc
    15001 cctcttaaaa attgtaatac atgtctgaat gtcaatatca agggaatcga taccaatgta
    15061 gaacctatta cactaaatac ataaacccat ataggattta agcccattgc aatacctata
    15121 ggaatggctc ccctcaattc tgttacagga accatagata aaattaatat gtgtaaatat
    15181 cccaaactat tccctcctat tctaagtgac attaagttat gaatttattt gctctataac
    15241 ctcaccatcc tttttactaa ctaaaaatgc atttttagtc aatgaaagca ttggtaaatc
    15301 actcttagag tctgaatatg catatgaatt ttcataatca atctctatcc caagtttacc
    15361 taatacagcc tttattcttt ttaatttttc aattccctta caattttctc caatgaattg
    15421 atttttatat cttgaatttt cataaaaaaa ctccgtgcct ataacctcgt ctgcaagacc
    15481 ataatactta aaatatttca tatatgcata tggagatgca gttatcataa ttataacatt
    15541 atcttcttct ttatttttgt aaataacctt tttcatagat tcgttaattt ttttcattat
    15601 gcaatcatca aaaaactttt ttaaatcttc ttcactaaaa tctactattg gataaaagat
    15661 tgcctctttt gccttcttca aaggtatcat tctaattaaa tacataaata gaataaaaat
    15721 aatgtatggc aactttataa tagttttaaa ggttttattt tttaaagagt aaataaaaaa
    15781 atctatcatg ctatcagtat ttataacagt ataatctaca tcaagtaata atacactttt
    15841 tttcataagt atttccccat cctatcacta aaaatatcac taaaaaatca atattattaa
    15901 acatgtaatt atccataaga ttacgtttat ttttaaagca ttatctttta ggacaatatc
    15961 agtaggatta ttttcatttg tagtgttaat tatataatta tatctaagta caccataaac
    16021 tacaaatatt gttgtaaata tcatatgtgg cttttctgga ttacttgtag aatacaatgc
    16081 ataacataca attgtagaac ttaatataac tatcatcatt tgatttagat tttctatgtc
    16141 ataatcttcc aatatttttc tatgttcaac tgcatcatct cttagagttt ctatttcttt
    16201 tttacgtttt ccaagagcta agtacagaga taagaaaaat gtacataaaa ttatccaatt
    16261 tgataaacta acacttatgg ctacacatcc tgcataaacc ctaagtataa aaccaagaga
    16321 tattgaaaac acatctaata gtattatatt ctttagttta agagaatata atatattgtt
    16381 tatcaaatag agactgagaa ttatagaaag attaaaacta acaactgttg ataaacagac
    16441 tgaaattaac gctaatacta caaatagtat attagcactt tttttgctta ttcttccact
    16501 tgccactggt ctattacatt ttttaggatg ttttttatct ttatccatgt ctatagcatc
    16561 attcagtgca tatactgatg atgacattag gcaaaaacac acaaacgcaa taaacgtatt
    16621 taaaaataaa tttatattta gtatgttgtt tgaaaaaatt atggctgaaa atacaaagaa
    16681 attctttata tattgcttta ttctcattaa ctttatataa tctgaaattt tagtaagaag
    16741 ccttatactt gtattggtct gtttcataaa cttactaccc tttcaatatt attttttaaa
    16801 caggatatga ttttatccct atgtttaaaa tttctataaa tccattgaca ttactgatta
    16861 ctatagtaac caataacagc gatagaaata tattatacat attcttttca ttttttctaa
    16921 gagctttcgt aaagtaagcc aataaaatta taactgcaaa actaaaattt atagccataa
    16981 tacttgaatt gactagatta tatcctacta taccatgtaa agttagattg aatataatta
    17041 ttcccataca tagcttagga actctttttt ctgaattttc aataactgat aaaacacaaa
    17101 aacataaagc taataatgta agaattactg gtattgtagc aacttctcca ttttgcaaaa
    17161 ataacccttt tttaatactt gctccaggaa aataaaaaac cgaatacaat aatctctcta
    17221 taatcattgc aaccttttcc aaaattggtt ctccacttat aaatttcgta tcatactgta
    17281 ttacatttcc aaggttcata aaactctcat aagacttcgt tgaaactaca caaatatatg
    17341 ccattaggat aaatgtaatt aaatgtttta acatttttcc tatttttaat ttaaactcaa
    17401 atatcatatt aaaaaaatac aaatatatat tactaatagt tattccaaat agaggaatcg
    17461 ctgctaattc tactaaataa gaattttttc ctttcatctt atttgcaata actatgaaaa
    17521 atattaatga aaattggcta tatataaatc tctctacatt taaagtcata aataactgtg
    17581 gaaaagaaaa tatcattatc atagtaagta aaattaaagt taatctctgc atttttatct
    17641 ttttaatatg attaaagact aaagttattg acatagcact agttattatt tgtagacaaa
    17701 ttagtccaaa cccataggca ctattaaaat ctgttgtccc aggatttgct attgaagtac
    17761 aaaataggta tattggatat gcaaaaattg atataattgc tgaaaacaat atatgcttac
    17821 tattgtcttg acttatagca aatacattta aattgaataa aactcctgta tctgtatcta
    17881 acaatacatc ataagtattg tttaaatcat aaaatatagt agtcttagaa aaaaatatct
    17941 tagttattat taagtaagtt attacaacta atataaacaa aaacatctcg tttttatatt
    18001 ttaatatttt actcatcaat atatcaccat ccattcatta tataggattt gctgacacct
    18061 acaatttatt ccttatattt aatagccatc ttcataatgt tcttacttgt tttattgtaa
    18121 ttgaataact ctttatttac aaccacaata tttactttat tgatgttata atcagtcaag
    18181 tattgtttat ataacataga ttagtgttgt ataaaatgag aatttgtaag caatattaat
    18241 gtaaaatatt tgaaaaactt tatataaaaa tatacaatgt tgcttatgga attaaactat
    18301 aacaaattaa agcataattt aaggagacaa aattataatc tgtctcctta aatattaaaa
    18361 ctatatttta aaacattact aaatactata caaatttcaa atatagtata aaattaatta
    18421 gactttaaca ctattagctc catcttcttc ccatttttta atttctttta atctatctat
    18481 tataggtaaa tgctgagtac attcaacttc acattttcca caagatatac attctttagc
    18541 ttttgcctta ctattcattg tgtaattcca atgtcctaag ccataaacta actttgtcat
    18601 ttcttcatca ctctttttaa acatctgctt ttcattataa aaaagcatat aggctggggt
    18661 attaatacct tttggacata ctttacaata accacaacct gtacatattt cgttcatatt
    18721 cttatttaat ctagtctcaa tatcatgtat atcctcatct gaataaattc tattttcgtc
    18781 tgcaatttta catgcatcat ctatatcttg ttttttatta aaccctatca aagatatact
    18841 tatttgtgaa attcctatgt tgaatcttaa agctgattct atagtacttt cgccatcttt
    18901 tgatagaaaa gaaagttctt tattatactt tggaatagtt ccaccactta atggattcat
    18961 tgccacaaca ccatatccat tatcaacagc atatttggca ccttttagcc tatatggaaa
    19021 gtttaatata tttattccca tagttacacc ttcaaactta ttttcactta gaatatctat
    19081 aacctcatct cctggttgat gagatgaaaa tactatatgg tctatcaatc cttcttcttt
    19141 acatttcaat aatccttcat attgacctcc aggtctcatt gctagttcgt aatgttccat
    19201 ctttctaata ttccaaacat gataaaaatt tattttagat acgcctaatc gttctattga
    19261 tttttttaca gcctctatag ctttttctga tgtatcacat gcttttggct tttctttagt
    19321 tgatacataa aaatcggtct taccttcctt tatcatttgt ctaaaggcta gtccaaatat
    19381 atcttcactt ctgtcatcac aataccctgg tgctgtatca aagtaattaa tacctttttc
    19441 atacgcatat ttgactaaat cagcattttc ttgattactt ttttctaaat caaaccttaa
    19501 tcctccatag ccaactactg aaaccatttt ccctgtcttt ccatattcaa cgtatctcat
    19561 tgtaaattcc tccttagata tattaagact taatttcttt atagaaaata ttataccata
    19621 aataattttt ataaattatt tatggtataa tatttttttt ataaattata tttttttata
    19681 acttgtattt tataactaag ttaaaaacta taaaaaggtt caatatggac tattacttga
    19741 gcattttcat taatttcttc ttgtatttgt ttctcaattt tatgtgttaa atcatgagat
    19801 tgctctacgc ttataaatgg gtctaccatt acatgcatgt caatataaat agaacttttg
    19861 ctacctctac ttctgatatt gtgaacatcc cttacctcat taaattcaaa cacaatttct
    19921 tttatataat cctcatctac agccactcta tcgaccaaaa ttccaataga tggcctaaat
    19981 attccataag ccgaataaat tatgaatgca gaaataataa taggaactat agattctatt
    20041 attactggaa atcctaactt aacaccaact aaagtaacca atacacctag agacacaaat
    20101 atatcacttc ttgtatgcaa tgaatccgaa atcaaaacat aactatttag tttagtccca
    20161 attctatatt catacatgca tacaaaaata tttataaaaa gtgtaataat taaagcaatt
    20221 aaactttcta tagttatagc aggtacaact ggattagcta ttcttaacac tgcactaaga
    20281 attatcttaa ttgctataac aaacaacata ccacttataa ataaactggt tataatttca
    20341 aactttttat gtccataagg atgattttta tcttttggtt tagatgcaaa gaaaatacct
    20401 ataagcccca ctatattaga tgctccatct gacaaagaat gaaatccgtc tgctgtcata
    20461 ctatagcttt ttatttgatt accaataatg atttttaata atgctactgc aaaattagca
    20521 aataaaatta tccacaatac ttgcttcact ttcttatagt tatccataac aaattccccc
    20581 tcccttcgat tcaataaaga aagctatcta tcaatatcaa tcaaccagac aataaaatcg
    20641 tatataagca catttactat atttatattc aaaattggtt gtacttgttc attctatttg
    20701 aaacttaatt ataaataaaa atcgtaggta tggttttggt atacataaaa ataagagact
    20761 gtctcaaaat aaagataatc ttctttttta ttgttgaaat gataaaatat atttgatttc
    20821 atataaaaaa gagtgcctca tgaactaaaa agttcatttt gagacactcc cttaaatttt
    20881 tgatataaac tattaatttt ttatactaag ccttctttaa ctaagaatga atgagcaact
    20941 tcttctggtg atttaccaag ttcatccact tgataattaa gttctctcat agtttcttca
    21001 tttatttttc ctgaaagcat attcataact ttttctaact caggatattt cttcaaagta
    21061 tcttctctta ctaatggtgc tgcataatat ggtacaaaga aatttttatc gtcctctaat
    21121 atcttaagat taaacttttt aataagacca tcagttaaaa atgcatctat aacctgagat
    21181 tcattattat ctaaagctgt atatcttaaa cttccattca ttgcttttac atctttgaat
    21241 tttaaaccat aatctctaga aagccctaca agaccatctt ctctattttc aaactccaaa
    21301 gttggtgaga atgtaaattc tttaccatat ttagtcaaat cagaaatagt atttattcca
    21361 tacttttcag cagtttcagg catcatggct aatgtgtaag tattattaaa tccaagaggg
    21421 tcaagtaaag ttaactgatt ttctttttcc atagtatctt ttacaacatt atatgcttca
    21481 tcagcatctt taatcggagc atgtctcatt acatttacca aaagtgtacc tgtatagtca
    21541 acatacatgt ctaactcacc agatttaatt gcattaaatg caacagatga accacctaga
    21601 tttaaattct tcttaacttt aagattcgta tttttttcta ctaattcagc ataaacattg
    21661 ccaagtataa gttgttctgt ataatcttta gaaccaatag ttattgtatc ttttttactt
    21721 gaaaaactag aactaaaaac aacgaacaac attgaaacaa caagcactac agatattact
    21781 ttaagtgcta tataattctt cttaggtgct tttggattta atccttttgg agtaactgca
    21841 acttcaattt tactaaataa ataatcaact aacagtgcaa ttatacatga tgggattgcc
    21901 cctgctaaaa tcatattatt atttacagtt tgaacccctg aaaatactaa atatcccaaa
    21961 ccaccagcac ctataaatgc tgcaagagtc atcaaaccaa cagctgtaac tgcagatatt
    22021 ctaactccac ccattattat aggaagcgct aaaggaaatt gtattttaaa taatgtctga
    22081 tttcttgtaa gaccaatacc tttagctgat tctaatacta ctgggtcaat acttgttata
    22141 cctatatacg tattttttac aattggtagt aatgaataaa ccactaccat aaatatggct
    22201 ggtttacttc ctataccaag tataggtaca agaagaccca aaagtgccat agacggaact
    22261 gcttggacta agtttacaaa tccaagtaca aactttctaa gagatgagat tcttgaaata
    22321 attattccaa gaggaacacc aactattata gctattaaaa tagatgtaac agttagactc
    22381 atatgttgta ttaaaagctc tataatctta tctttttgta atagtataaa gttaaaaaaa
    22441 ttagccaaga acactcacct ccatttctaa gaattgttca cttaaaacag aaagtaaact
    22501 acttctagta ataagcccta ccagtttatt ttcatcagaa ataaccggaa tatatccaac
    22561 cgaattacga ttcattacat ttagtatttc tactaaatta tcaccttcat ttacatgcaa
    22621 aggctcactt gacataatat cagcaagtaa tattgactta tcatcaatat ctttcatatc
    22681 ttttacagta acaatacctt ttaatgtttt tgctctatct ataattagta agctatcaac
    22741 cttactcgtt ctcataattt ctattccttg agttacagtt ctagtagaat taacagcaat
    22801 aggatttttt atcataatat ctcttgcttt tatatattct ggattatccc aaactctatc
    22861 ttctccgata aaatctttta caaagtcatt tgcaggcttt ctaagtatat tttcgggagt
    22921 atcatattgt gctattcttc catccttcat aatacaaatt ttatcagcta tctttaaagc
    22981 ctcatccata tcatgagtta caaatattat agtttttttc aattcatctt gaagagaaaa
    23041 taattgttct tgtaaagatg ttctcgttat tgggtctaaa gcactaaaag gctcatccat
    23101 caatattatt tcagcatcag tagcaatggc tcttgcaaca ccgattcttt gttgctgtcc
    23161 accactaagt tctgaaggat acttattcaa aaattcatca ctatcaagtc ctaccatctc
    23221 aagtagttgc aacgttcttt tttcaatttc ttcaacggat ttttcctttt ttaggcgtgg
    23281 aattagctct atattttctt ttattgtaag atgagggaat aggcctgtgt tctgtataac
    23341 atatcctatt tttcttctaa gctctatttc attttcctga gaaattggtt tgccattaat
    23401 atatatctca ccagatgtag gttttattaa tttgtttata agcttcaaag tagtagtctt
    23461 tccacaacca cttgaaccaa tcaaaactac caatgtacct gtttctacaa ctagagaaat
    23521 atcatctaat ataacattat ttcctatttt ttttgttaca tttcttatct ctatcataat
    23581 ttatcatccc ctttttcgaa caagcaacct tcttcaatat tataagttgt ttttataatt
    23641 atatcatact attttattta tgtaaacaaa gtacgatttt taggtcacta tttgtctgaa
    23701 tattgctaca aatataaagt atttatatat agtatataat atataatgtc aaaaaacaaa
    23761 aaatataaaa atattcataa aataacacta gcctcaatta tatagtatta aagctagtgt
    23821 tatttcattt aaacaaaact cacatttgga tactatccat gtattcaaat aatgtaatat
    23881 ctttataagt ttctttttta tcacttaggt ttgatattgt aactcccaat agcctaattt
    23941 gttttccatt aacatcaaca tttctcaaca aattatctac accacttctt atatcattat
    24001 attcgtcaat atagtgttct aaactaaggc ttctagttat ctttgtaaag tcctcatatt
    24061 taatttttaa tgttaatgtt tttccaaact tctcagagtt ttttaatcta tgacatactt
    24121 cttcacaaag ttcatcaaga atgttgcgtg tttcttcttc atcaatatcc aaattatgac
    24181 ttaatgtagt ctcagcccca acagattttc taaccctatt tggttccact ggtctatcgt
    24241 ctattcctct agcaaattta tataattcat aacctctttt tttaaatata ttttcaagtt
    24301 caaataaatt taaacacctt aaatcatatc cagtttttat acctaaattt ttaagagtat
    24361 ttgaagtaac ttttccaaca ccaaaaaatt tattcacagg taatttatct aaaaagtcct
    24421 gggcattttc ttcggttatt acagttaacc catttggctt tattaagtct gatgcaatct
    24481 tagcaagaaa cttattgtat gacacaccag ctgaagaagt aagaccaact tctctaaata
    24541 tatctttttt tatttgcttt gctatctcaa ttgatgaatc aatatttttt ttattttttg
    24601 ttacatctaa gtaagcttca tctaaagata atggttcaac taaatccgtg tatctataaa
    24661 atatatctct tatttgctct gacacactct tataaacatc aaatctaggt cttacaaaaa
    24721 ctgcatatgg acacctattg taagctacag ctgatggcat agctgaatgg ataccaaatt
    24781 ttctagcttc ataagaacac gttgcaacca cacctcggct ttgtggattt ccaccgacaa
    24841 taacaggtct tccttttaat tttttattat ctctttgttc tatagatgca taaaaagcgt
    24901 ccatatctat atgaattatt tttctataat gtttcattat tatcaccttt taaagtataa
    24961 atagattaat tttatttata ttacttttta attataccaa acttatgttt ctttttggta
    25021 ttttttcatt attttcttta tatattcata ttttccataa ataaaaccat ctataatcca
    25081 ttaacttcaa tataatttat aaaaaatcct taaaaacact tgcaaaaact atataaaagc
    25141 tttacaatag gtattccaag gaactaaatt taaaattcct tatggataac agtatatata
    25201 tttaatttat gggatgtttt aaaaacaaaa gactgtctta tacaagacag cctactgatt
    25261 ttattataat tgtaatggac tgctcaaact attactttaa aacagcccat atttttttat
    25321 ataattttaa ctatagtaaa gattttgtga aggatattct gggtcacaag ttacatctat
    25381 accaagcttt ctaaatgttt gttcatcatc tctatttaat atagtagtag aatgagcttg
    25441 acaccctttt aataatgcta acttttccat agcaaattgt gcagttggat tagttacagc
    25501 acaaatacta agtgcagtta acacctcttg gcaactaagt gaaacatttc tactagctaa
    25561 agtctttgtt tttaagttta tgataggttc taatattaca ggtgatatta agtgcatatc
    25621 atcagatata ttggctaaat gtttaatggc atttaatact actgctgcag ttccattcat
    25681 taaatcagaa cccttaccag ttaatattgt accatcttct aattctaatg caactacagg
    25741 acaagtatca ttcttgtcgg cactttcttt caatttatta ctgtagtttc tagcaggtaa
    25801 tacaacattt ctatcttctg gctttaagtt aagctcttcc ataatcattt tcattctatc
    25861 ataagcacct ttatcaactt gtcccttttt atactcacag gcagttttaa agtatcttct
    25921 tattatttct tctatagaag cttttcttat tacttcatca tctattattc caaagccaac
    25981 tctattaaca cccatatcag ttggagattg gaaaattgat tcttttccag ttatcttttc
    26041 tattattttt ttaagcactg gaaataattc caaatctcta ttgtagttta ctgacatttc
    26101 accatacgtt tcaaggtgga atgaatctat catattcaca tcttttaaat caactgtagc
    26161 tgcttcataa gctatattca aaggatgttt tacaggtaca ttccaaactg ggaatgtctc
    26221 aaacttagaa tatccaacta cattacctct tttattttca tgatacaact gacttagaca
    26281 agtaccaagt tttccactat taggtcctgg tgctgtgact actactattg gctttgtagt
    26341 ttctatatat tcatttgctc catatccctc atcactaact atagtctcaa catctgacgg
    26401 atatcccttt gttggataat gcttataaac tttaattcct ctacgttcta atttatttat
    26461 aaatacagtt gtagcaggtt gtccttcaaa tctagtaatt actacactat ttacatctag
    26521 ttcataacct cttaaatcat ctattaatct taaaacttcc atatcataag tgataccaaa
    26581 gtctcctctt attttgtttc tttctatatc tcctgcatag acacaaatta caacttcaac
    26641 tttttctttc atatgttgta atacttttat ctttgcattt tcatcaaatc ctggaagtac
    26701 tctttttgca tgaaggtctc ccatcaattt tcctccaaac tctaaataaa gtttgtcaaa
    26761 attatttact ctttctaaga tatatttaga ctgctcttct aaatattttt tgtggtcaaa
    26821 tcctactttc atcttaacac ctcactaaat taaatttctt attgataaca gtatatttat
    26881 atatattttt ttagtaaaac atcttataaa aaaatgttaa tactttttca ctcaaagtaa
    26941 tattatacaa ttaagattaa tttttttcaa gatagtttta tggtaaataa gatataccta
    27001 atataactaa taaaataaga atactatatt tttatatata agtataatat tctcacctta
    27061 gcattaattt atttcaatat tttcatatat cttttattct aaattcaata cttttctaat
    27121 atcttctata ccttgataaa cttcttgttt agaaaaattc atagcttcta attcttcatc
    27181 agttttttcc ataagcatag tataaaattc tattgctata tactgcatat tttcactatt
    27241 tacactctta gctttatcaa ataaaatttc ttctgcttcg tttacatttc cctgaaaaac
    27301 taatcttttt aaagttataa gtataacttc ttcttctgta ttatacacta aatctttttc
    27361 ttcatattca ctgtattttt ttcccaataa aagtcttgca accataagtt ttatctcatt
    27421 gaaaatctta tccactatag ccatttttac atctcctata aataaatttt attttctttt
    27481 taattatatc agactttatc gtaaaaatta ttatatgtaa atgaaataat tcaaaaataa
    27541 attttaacaa taaaaaaaag aaggtagcgg gggatttccg actaccttca aacactagat
    27601 tgtgattggt ttggtatttt atttataccc accataagct ttattaaatc atctaaattg
    27661 atttttttag catccacatc ctccatcaaa aacgtcacca cattcacaaa gtattaataa
    27721 taatattaag aaaggtatcc atgcacataa gtccatttca gcccaacaat cttggaatgc
    27781 taagaataag aagaataata ttattatcca ccaagcacat ccaccaaatc cgccttcacc
    27841 aaaaaatcta tccaacataa tatgttacct cctagtcatt ttgtatgtgg agtttcagct
    27901 cctaaaatat actatgttta aggggtaata tttgctaatg atttttttaa atttttttag
    27961 atatttttaa aaaaattatt gattcacact caatgtctta ccatcactat tgataactac
    28021 ctctccatta gaagtcaaaa atgtttttat atttaatctt tccaatattt ttaaaacacc
    28081 ttcctctggt tcttttttct ctgaacatgt aataacagca tattggggtg aaatagactc
    28141 taaaaatgtt tcagttaatt tgtcatatct tccatgatga ggcattttaa caaaatcata
    28201 ttttaaagta tttccattta taaactctga caatctttct tcctctgcat ctcctgcaaa
    28261 taaaaactta tgtgctccat agcttatatt agtgattatt gaataatcat ttgaactctc
    28321 gtacttgctt tttgaagccg gatgtatgtt tatttctgca ctattgatta caatattcat
    28381 attatctttt agtagcactg gttctatatc agctttttta agtgagtcaa tatattcatc
    28441 atattgctta ctatctttct tataatttgc ttgtataata ttctttactt taacactaga
    28501 taacacacta tctactccac ctatatggtc cttatctaaa tgagttaaaa ttaaatagtc
    28561 aagtgtattt attccttttt cttttaacct atccactatt tgttttccat ttttatcttc
    28621 tcctgtatca atcattatga ctttgttacc tatctcaacc aatatacagt cagactttcc
    28681 agtacttaat atagtagtct taaatgcagt attttttgtg ctactctgaa tattattttg
    28741 actcacatca tttttgtcac tttttaaata agattcattc gttgatttta tcttactatt
    28801 agtagttata tttgttacaa gcaataatat tactattata gataatgtag taactaaaaa
    28861 caggatatct tttaatacta ttttcctcat ccaaaagctc ctataaattt tatttattac
    28921 atatttatag tatagcaacc ttaactctgt aaaatacaca tataaaaagg gcaaatttta
    28981 ggcatatttg tggcatgata aaaacatgat aaaaaaatga ctgtaccaaa acaattatct
    29041 ttctaaattt acatcttcaa attctttaat cttatcatta gataaaacac tctttttagt
    29101 agattgtcca ttttttctat caaaagtatt tttagctttt acacctgata aaaacttacc
    29161 tgctccatta atacaacctt cttcacacat cattccttct ataaaattac cttctaatct
    29221 tcccaattta gccattgtca ttgtcttttt aatttgtctt ggaccagata cttttatagg
    29281 ttcaaaatca aaactaagcc ccttttcagc aatatagttc tgtactgatt tggcaagtcc
    29341 accagatgct ccaaatcccc ttccaaaaat cgaaccatca tttatatctt catttgaaca
    29401 ttttgataaa tctatattaa atgcttcaaa taatgccatt agttcttcaa aagtaagtac
    29461 ataatctact gaatctttta aacttttctc tgttgcctca ctctttttag ctgtacatgg
    29521 acctataaat acaactttag catcagaatc tatttttttt actaatcttc ctgttgctac
    29581 cataggagac actgtagatg atattctatc tttttcatct gggaacatct tctctatata
    29641 cttaaaaaat cccggacaac atgaatttgt catgtaatta tctcctgatt ttattctttc
    29701 aataagttca ttgctttcat gaactgtaac tgcatctgct ccacaagaag cttctatcat
    29761 atctacaaat cccagtgatt ttatagcatt ttttaactga ccatatgtaa tattactatc
    29821 tatttgacct gttatagctg gagctactat tgcatacata ttttctttct ttgcaagttt
    29881 ttttgcaaca ggtgctatca aactcttatc agatattgct ccaaaaggac atgctgacat
    29941 acatgctcca caatttatac aattatcttc atgtatcatt gctttcatag tgtttctatt
    30001 aaaatccaaa gctcctgttg gacaaacgct tttacaaggt cttacaactt ctgatatagc
    30061 atcatattga catgaacttt tgcacattcc acaagcctta cataaatcat gatttatata
    30121 tgctctccca tttatataag aaattgcccc aaatttacat gcttccttac atctatgagc
    30181 caaacaccct ctacaaagct ctgtaactgt aaattcattt attggacatc tatcacatgc
    30241 tgcttcaact atatatataa tttgctcatc tgtgtccaaa ctaactgaat ctggatttat
    30301 atctccatat ttactatttg gagttaaact taatgcaagt tgcgcccttt caagaactac
    30361 ttctctctct ttataaacac aacatctata tctcgccttt tctcctttaa ttatttcata
    30421 tggaattttt tctatatttt cttttgttaa attttcttca atagatagtc ttgctactgc
    30481 tgttaaaact tcatgtttta acctttttag ttgcgtttca aattcaaaca taagtaatcc
    30541 tccctataaa tcacaccttt ttgctatatt tctactatta tgtatattgt attttttatt
    30601 tatttctata aattattata acataggcat atactttgtt aagttatata caaaatatta
    30661 tatatgtaat ttttatttta agtgtatata atttttattt tagttgttca ataattaaat
    30721 atataaataa aaatagagtt gtccaaaagt taaactttaa cttagaacaa ctctattaac
    30781 aatatgtatt tttgttgctt tgtattatct ttatttttct aaatttatat tactaaaatc
    30841 ttttatcttt gtatttgcta agacactttt cttagttgat tgagaatttt ttctatcaaa
    30901 agtggcttta acctttgcac ctgataagaa tttaccagaa ccatttatac aaccaccttc
    30961 acacatcatt ccttctataa aattaccttc tagctttcct aaattagcca tagtcatagt
    31021 tttcttgatt tctcttgaac cagaaatctt tacaggttca aaattaacac caataccttt
    31081 ttcagcaaga taattttcta tagcttttgt aagaccacca gcagctccaa aacctcttcc
    31141 aaaaattgat gcatcatcta tatcttcatt tgaacactta gccaagtcta tatcaaatgc
    31201 ctcaaataat gccattattt cttcaaaagt aagtacataa tcaacagctc cttttaattc
    31261 tttcttagct gcctcactct ttttggctgt acatggtcct atgaatacaa ctttagcatc
    31321 tggttcatga ctttttataa atctaccact agctatcata ggagacactg tagaagatat
    31381 ttttcctaca tgatttgggt atgttttttc tatataacca acaaatcctg gacaacaaga
    31441 atttgtcata aaagcttctc ccttttccat tctttcagca aattctaagc tttcatgaac
    31501 tgtaactgca tctgctccac aagctgcttc taatacatca acaaatccta gtgattttat
    31561 agcatttttt atttgaccat atgtaacatt tgcttcaact tgacctgtta tagctggagc
    31621 taccaccgca tacattttct ctttttgaac taactttcta gctacaggag ctatcaaact
    31681 cttatcagat attgctccaa atggacatgc tgacatacat gctccacagt ttaaacattt
    31741 atcctcatgt atcatagctt tcatattttc tctatcaaat cctaaggcat tagttggaca
    31801 aacactctta catggtctaa caacctctga gatggcatca tattgacaag cttttttaca
    31861 cattccacaa gccttacata attcatgatt tatataggcc attcctccga catgtgttat
    31921 agctccaaat ttacaagctt ctttacatct atgtgctaga catcctctac aaatatctgt
    31981 tactgtaaat tcatttattg gacaactgtc acatgcagct tccaaaatat atataatttg
    32041 ttcatcttct tctatatcca ttgtatctaa atctatctta tcttgacctg gttgtaaccc
    32101 catacttaca tttactctct cagatattat agctctttct ttataaacac aacatctgta
    32161 tgtaggtttc tcacctttta ttatttcata ctgaatatta tccattgctt ctctggtaag
    32221 gttatcttct tttccaagtc tagctattgc agttaaaaca tcatgtctta atttgtgtat
    32281 ttgtgtttca aattgaaaca ttttaatacc acccccgtaa atgattttat acgataccat
    32341 tataacacaa tatgtccttt tattaatata tacataaaat attatatgtt taaattttat
    32401 tttatcgaat atatatagtg tttttttgtt ttattatata atattattga tatttctgaa
    32461 atttcactat ttttattttt acttttcaat ctaatatata atatttttta ttatatattt
    32521 taattttact ttttacttac ataccttttt gttacatttt ttgatattaa atttattatc
    32581 tacatttaca ttttcgtcaa attttacaat acttctcact gtataattta acaattttta
    32641 ttaaattcta aaagtaatta atttttttaa aaattttatt tgcttttatt taaaatttat
    32701 gttatattaa tagaacagac ttattttttt agtttgtatt ttttaataat gcggaggttt
    32761 tcttataatg aaaaacggaa tagtaaaatg gtttaacaat gaaaaaggat ttgggttcat
    32821 atctgtagag ggagaagatg atgttttcgt acatttctca gctatacaaa atgatggata
    32881 caaaacttta gaagaaggcg aaaaggttag ctttgatata actcaaggta atagaggacc
    32941 tcaagctgaa aacgtaaata gaatataatt atttaattta aatatatata ttttagaaaa
    33001 ggagctatcg agggctcttt tttgttgttt attatattta tttttaatta caaaaataag
    33061 aagttacttt agaaaagcgt caaacttaaa ctaacttttt taaaataact tcttatatta
    33121 taatatatac tatttatata aaatactcta tttaatttta tcagatatta ttaagcataa
    33181 ctctcttaag attttacaag ctataactgt cgatacacct gttgtatcgt aatcagggct
    33241 taactctaca atgtcacaac caactatatt tatattagag ttttttataa tcttaaaaat
    33301 ctcttgaaac tctctataat ttacaccacc aggttctggt gtacctgttc ctggaaacac
    33361 agatgcatcc aatacatcta aatctatagt tagataaata ttctttccat ttagtatatt
    33421 aactatattt tcaaacgtat ctattcctcc tatttccatg tatgtgtgtt tttcttctgt
    33481 agcaaattta aattcttctt ttgtcccaga ccttatacca aattgaaata ttttattatc
    33541 acctacaata tcccatattc ttttaattac tgttgcatga gaatttttac tattattata
    33601 ttcttccctc aaatcagtat gggcatcaaa atgaattaca tatatatcat tatacttttc
    33661 atgtactgct ttaaaggctg gtaatgtaac taaatgctct cctccaatca taaagggtac
    33721 ctttgaatct ctaacaatct tatatgtctc ttgatagatt tcttttaaga cttgttctgt
    33781 acttccaaca ctaatttcta aatctccata atcacatata ttataatcct ctaaatccaa
    33841 atctaaaaaa ggactatatg tctcaagacc gtaaaattct tttctcatag agctacttgc
    33901 aaatctagcc ccaggtctgt tagaagtagt tccatcaaat ccaacaccaa atactataag
    33961 attagattct tcataatttt tgtccatact cataaaagtg ttcatatgat agaaattatt
    34021 cttcatctag catctccttc acgtattgag gtaacataaa cgaacctaaa tgtatatcac
    34081 tattataata tttagttttt aaacttaatt tctcccatcc atttctatct tgatttttta
    34141 ctggattaag tttttttgaa gcaaatccaa acaaccagtg accagatgga tatgttggta
    34201 tatgtgcttg ataaacttct gatattggaa atatctgttt taatttttta ttagctcttt
    34261 tcatctcttt agcattaaag tcaaaatacg gactttcact ttgatttact aaaattccat
    34321 catcagtcag tattctataa caatccttat aaaaatcagt tgagaaaagc ccttcgcctg
    34381 gtcctattgg gtcagttgaa tctactatta tcaggtcata tgatttatca tgtgcacact
    34441 taacaaaatt aacaccatct tcaaaatata aactcactct tttatcatct aatttacatg
    34501 agcatatatc catatactcc ttagatacat ctacaaccat tttatctatt tcaaccatgt
    34561 caatcttttc tatttgagga taacggctta attctcttac tgtacctcca tcacctccac
    34621 ctatgactaa tactttcttt atgtttaaat ttgtagccat aggaacatga gttatcatct
    34681 catggtatat aaattcatct ttatatgtta ccatcattaa tccatctatt gttagaaatt
    34741 ttccaaattc atcactatca aacacatcta tcctttgaaa ttgagacttt ccttcaaata
    34801 gatgtttatt tacttttata gaaaatctta cattttctgt ccattcttct gtataccaaa
    34861 gttccatata ataatcctca ctttctcttg tatgttttat actgcttctg gtttatgagt
    34921 aatcttacct aaatcttttt ttgaatattt tttaattttt gtagttaagc ctcttggaat
    34981 ttctgtagac tcgcttcttt cagactttaa aagttgctct aataattcaa aacttttcca
    35041 tgggtctata tcaccacaag taaaaaaatc tgctgctgca taaccatatt ccggccatgt
    35101 atgtattgtc aaatgagatt cagcaattat aacagctcct gaaacccccc aaggattaaa
    35161 atggtggaat acagaatcca caattgtcgc ttttgcattt aatgctgaat cattcataaa
    35221 attttctata agacgtgggt ctttaagtat gttttcatca caattatagt attctactag
    35281 tatatgtctt cctagtgttt caaatttcat ttcaacccca actcctttat acttttaata
    35341 agttctattt gataaactct acttaacaaa cacttgccct tgcaaaataa agtaatttat
    35401 tttattacta gtattctatc taagttttta tcttgtacat ctgtaagata cgcattgcta
    35461 ttttttaaga aaataatata ctccataatt tcttctgtta tcaattctcc tggagcaata
    35521 atcggtatac ctggtggata tgccattatt gattctccac aaattctatc tatacatgaa
    35581 ttgatttcta cactttcctt tggtgcataa aaagcttctc ttggattaag tttaataatt
    35641 ggatttattt gaaccatatt aaattcattt aatgttgatt cttttctata aatctttgca
    35701 attatagaaa gtgcctttac taatctatct atatctgatt tatttgttcc aatggacaca
    35761 agtgccagta tattatatga atctcccaat tctacttgaa ttgaaaaatt tttatataat
    35821 aaatcgtata cttcaaatcc agtcaaattt aattctttta catttataca caattttgtt
    35881 tcatctataa atttaacacc tttttgtttc aatatttctg tagataaaac ttttattcca
    35941 tcaattttgt ttaatttaga ttttgcatac ctagatagat ttaatgcttt tgacagttgc
    36001 ttttcaccat tctcaactag attaactcta gcaccatcta tacttgccat aagcaaataa
    36061 gatgctgatg ttgattgtag catattgatt gattgaagaa ccttttcaaa acttacatta
    36121 tctctattca ataagagtgc tgaagattgt gtcaatgctc ctccagtttt atgtatactt
    36181 acagctacca tatcagctcc caaacttatt gcagaaggag gtaaatctaa atgaaatgga
    36241 aaatgtgcac catgagcttc atcaactaac actaaaacat tatttttatg gcatagttct
    36301 ataatactct caaggtctgc acaaactcca tagtaagttg gattaaggaa aaataatgct
    36361 tttatattac aatctttttc tatctctgtt ctaacttttt caagagtaat atttaaactt
    36421 atacctagtt tttcatcaaa ttctggttgt ataaatattg gtattcctcc acaaagtatt
    36481 aacgcattta ttactgattt atgtatattt cttggaagta atactttttc acctggtttt
    36541 ataacactca aaatcattgc atgtattgct tgagttgtgc cattcgttat aaagaatgcc
    36601 tcatcagcca tatatgcttg tgctagcaaa tcttgtgcat tttttataat cccatttggc
    36661 gcagatacat tatccatagt tggtgatgaa tttatatcca ttttcatgag actttcacct
    36721 agatatttat ttaatatagc tacacctcta ttttttacat gtccaggcac atcaaaacaa
    36781 gctatatctt tctctgagta ttcctttaac gactctaata atggagcaca ctcatagctt
    36841 aattcaactg catttgcaaa ctcgctatcc atgatttcaa attcatctcc tctctatatt
    36901 caaatatttt taatatttat tatttattat ttctattttt tatttttact tttattaaac
    36961 tcatgatatt tattatatta acatctcagc atttttacaa gtgttttttt gaagttactc
    37021 aaaaacatat acattagcta tatcaatatt tataaacttt ttaatttttt gttgtttata
    37081 ttaatttttt taaaacttag tatttataaa ctttcttatt ttttattatt ttggtttttt
    37141 tacttttttg agttttagta agcttttgag tttactattt ttaatttata agtattttat
    37201 tactaaactt ttatattatt cttatttcac atattaaaat tcttggttta gttggactaa
    37261 ataattttcc tatttttgta ttttaatttt aatataaaat ttattaaatt ttttaattga
    37321 taaaataaat tctatattta tgatattaaa attctataag ttcattgatt tatatatgtt
    37381 aatttaaata cattattcta tcgtagtgcc ttagagtttt tatttgtatt taaagttaaa
    37441 tttattcttg tttttccttt aatatttaat agtaaaacaa caaaattata aaaaaaataa
    37501 agagaaactt tataaaagct tctctttttt catctcttcc ataggtatcg ttttccaatg
    37561 ttctctcaaa actctttcaa cattttctaa atcattctcc ctcatagatt ttactacttc
    37621 aatatgttga ttattgctgt atgtcaacat tttatataac tcatcttcat tatcactagt
    37681 atatgacttt gacataaaat tatatcttaa agagttcaat agatttacta acacctgatt
    37741 atctgattta gttatgtata tatcgtgaaa ttcattactt aattttaaat agctattgta
    37801 atttttatac tttatagaga ccgccatcat ttctgttaat tcttccattt ttactaggtc
    37861 tgattcagtt aaattttgta atgctgaaga accggctaac gcatccaata ctccaattac
    37921 ggaataggtg tctaatttct ccttttcctg aaattctttt actacaaatc ccttattagg
    37981 tattttttct agtaaatttt cacctgctaa cctagttaat gcttctctcg ctggtgttct
    38041 actaactccg atagatttac aaatctcaga ttcagtaatt ctctgattag cttcatattc
    38101 tccattttgg attttctttg atatatagtc atatacttga gttgtaagtg gatctacctt
    38161 cataagacgt tcctccatat taagattata aaagttaaat ctccctacaa ctttggtaga
    38221 gagttcagtt ttatcttact aatttatata atatcacaat tatgatattt tttatacttc
    38281 tttgtaatat tttgaaatac taaaattctt atgataattt atacaaaaaa agtagaaaaa
    38341 gttttgataa aacaaaactc tttccacatt caccttattt tctatttatt tttctaaaaa
    38401 aagatattta atttactcta aaaattctaa atgatttaga gtctaaatct actttcaact
    38461 cattattttc aatttgtata gtactatttg catttttaag actatacttt ataggtattt
    38521 cctcaataaa actactctct atacataatg atatactttc cacattttca cttctattta
    38581 ctaaaattaa tattctatca ttattttcat taactctaat aaaagaaaat acagaatcat
    38641 tattagtata tataaattct gtatttccag aacttaaaag tttattctta tttctaattt
    38701 ttataacatc tctataaaaa ttaagcatat cttcgtcttc atttttccat ggataagttc
    38761 ttctattatc tgggtcaaca cctccacaaa gacctgcttc atctccataa tatatatatg
    38821 gtactccttc aaatgtcatc tgtatagcta cagcaagctt cactaaatct acatcatcat
    38881 ttaattccgt tttagctctt gtaacatcat gtgttccaat taaatttaaa ttagacctaa
    38941 atgattcttt tggataattt tctttaatct gcatatatct attacaaagt tcatatgaat
    39001 taatttctcc ttttaagaaa gaaaacatgt tatttctaaa aggataaccc ataacagaat
    39061 ctaattcttc acctaataaa taacttcttc tctgaccata gctaatctta tttgatgcat
    39121 cttcccatac ttcacctata agaattgagt catcatctgc cttttttaat tcctttttta
    39181 actctcttat aaacttagtt ggtaattcgt ctgcaacatc tagtctccag cccttaatac
    39241 ccatatttac ccatttattt ataacgctat cattgtcata tattatataa tccatatagc
    39301 tatgttctaa ttcatttata tttggcaatg tcttaacatc ccaccaactc ttatattttt
    39361 gaggaaattc ttcaaacata taccatgaat aatatggaga ttctttagat tgatatgccc
    39421 ctaaactatt atagtttcca tacatattaa aatatttact atctgctcca gtatgactaa
    39481 aaactccatc taatactatt gatatacctt tttcttttgc tttatcaata agttctttaa
    39541 atgtatcttc atccccaaac attgggtcaa tttttttata gtcacctgta tcatacttat
    39601 gattactaga ggcttcaaat atagggctta gatatagtat acttacacct aattttttta
    39661 aatatccaag tttattgatt atccctctta aatttccacc atggaaatcc catcttataa
    39721 catccccttg actatccttt atgtacatag gtgtatcttc ccaatttcca tatatgaaac
    39781 tatttttttt aggattatca accttaccac tacgatttcc attattgaat ctatctacaa
    39841 atatatgata aagtattcct tctttgtacc atgttggtac tttaaaatct ttgtaaacag
    39901 tgatttgata tttattaata tcactataat tatattcaca agaatatcca ttatctctgt
    39961 tttttccata aaataaagtc tttatctgac catccatttc aacatcaact ttaaagtaat
    40021 aaaaataaac acctgtacca tcaaactttt caatcttacc acaaaagcac tttccaagtt
    40081 tatcattatc taattcttca tttaaagtta ttgttctcat ttcttcttct ctatttattt
    40141 ctagggaaat agacttgata ttgtatccct catttacttt aacacaaatg atcaattcct
    40201 catcaaattt taaagctcca aacggagcct taaaattttt atcccaagag ttatattcta
    40261 ttacactttg catttaatca caccctttta aaccaaatgt cttttgcata ctcagaaatt
    40321 gttctatcag aagaaaaaaa tccagagtta gctatattaa ctaaagacat tttattccat
    40381 ttttctttat ctctatataa cctgtttata tctgcttgag cttgaccata attttcaaaa
    40441 tctctcaata caaaatactc atcattgtaa gtagtgagtg aattataaat acttcttcct
    40501 tcttcgccaa gattaggaat aaatccattt attaaatcat ctactaccct ttttatatcc
    40561 atattagaat gatataggtc aagtgaagaa tatccaccat atttattata atttagtact
    40621 tgttctgcac taagtccaaa taaaaacata ttttctttac caacctgttc acatatctct
    40681 acatttgcac cgtcaagtgt agccaaagtt atagcaccat tcatcataaa cttcatatta
    40741 cttgttccag atgcttcctt agttgtagtt gaaatttgct cactaacatt tgctgctggt
    40801 attattattt ctgctaatga tactccataa ttctctaaaa atactacttt taacttgtcc
    40861 tttactctaa catcattatt tatagtactt gcaacagaat ttataaattt aattatacat
    40921 ttagccaaat aatatcctgg tgctgctttt gcgccaaata taaaagttct tgggtcaaga
    40981 ttcaaattag gattatcaag taattcatga tacatatgaa gtacattaaa tatattcagt
    41041 aactgtcttt tatacgcatg caatctctta acttgaacat caaatatcga acttgggtct
    41101 acatttaaat cgtatttatc ttttataaac tttgctagat ttgctttatt attgtatttt
    41161 atatcatcaa gtctttgtaa aacagaggaa tcatttttaa atttttcaat attttttaat
    41221 tgtaatgtat ctttcttcca tgaatcacca attaaatctg taattaaatt gctaagttga
    41281 ggatttgaac ttataagcca tcgcctatgt gcaattccat tagtcttatt attaaattta
    41341 tttggttcat cttgatagaa atcttttaat acctctgttt caagaatatg agtatgaagc
    41401 ttagcaactc catttacact atgacttgtg acaatacata agtttgccat attgatattg
    41461 tcacagtcta ttatactcat tctttttatt ctatcttggt catatccttt attatttaat
    41521 tcctctacat atcttctatt tatttcttct attatcatat aaattcttgg taatagttct
    41581 ttcatcatac ttacaggcca tttttccatt gcttctgaca ttatagtatg attagtatag
    41641 gacactgttt ttttagttat ttcccatgct tcatcccaag ataaattttc ttcatcaagc
    41701 aaaattctca taagttctgg tatgcataag gtaggatgtg tatcatttat atgaattgct
    41761 accttatcga ataaattatt aatatttaat ttgttctttt tatattttct tattatatct
    41821 tgtatccctg cacttacaaa aaagtattct tgtttcaacc tcaacaactt acctgcatag
    41881 tttgaatcat caggatataa tacctgtgat atttcctcag tataatactt atgttttaaa
    41941 gcctcttcat agctaccaga ataatttaga gcatttgagg taagttcacc aaaatctctc
    42001 tttggtattt cactcttaaa caatcttaaa gtatttatac attggttttg gtatccgatt
    42061 attggtatat cataaggcat tgccattaca ggaaggtagt ttttatgaat tacttttaaa
    42121 tgactacctt ccttgattaa ttctacctca ccaccaaact tcaccattgt tgcttcattt
    42181 ggtcttacag tttcccatgc atatcgtcct tctgtaagcc agttatcagg cacttctacc
    42241 tgataaccat ttacaaattt ttgttcaaac agaccatatt tataccttat tccatatcct
    42301 tgaccactta tgttcaaaga tgccatagaa tctaaaaagc aagctgctaa tctaccaagt
    42361 ccaccatttc ctaaggctgg gtctacttca gcttcaatta aatcatccaa gttgattcct
    42421 aattcagata aaccttctct aacttcttct tcaatattta aatttaataa attagatttt
    42481 aactgtctcc ctattaaaaa ttctaaacta aaataataaa cttctttttt cttatctaac
    42541 tttgtagcaa cccatttttt tgctatctca tctttcaata gactacataa tacatttaac
    42601 aattgttcat cagtagcttc cttgatagat tgtgcatata agctatacat ttttacttca
    42661 aatttttgct tgaatttctt tttgcttatt gttaccaaaa aaaccactcc tttaatcaca
    42721 tatgtctcta tatgtttcta tatactcctt agctgatttt ttccaactat tatctgtttt
    42781 cattgcattt tcaactaatt taatccattt ttctttgtcc ttaaatactt tgattgcatt
    42841 tttcaaacag aaaaacatct catgagcatt gtaattttta aaactaaatc cattgccttc
    42901 tccagtaaat ttattataag gtgttactgt atctctaagc cctccagtct ctctaactat
    42961 tggtaaagaa ccatatctca tagctaacat ttgacctata ccacaaggct caaataatga
    43021 tggcattaag aacatatcac ttgcagcata tatctgttga gccaatgatg cattaaaagt
    43081 aattctagct gatactttat ctgaaaaatt agaatcgtaa tagttgaaca ttgattgata
    43141 ttgatgttct ccagttccta aaacgacaat ctgtaaatct tctctcataa gttctggcat
    43201 catgtatgct attaaatcaa atcctttttg agaaactaat ctcgaaacta tacctatcaa
    43261 cggaatagat gaatcttttt taagtcccaa tatatcttgt aatctcaatt tattttctac
    43321 ttttttgtca attgagtcta catcataatg aacaaatata tctttatctt ttgcagggtc
    43381 atttaaatca taatctatac catttaatat accttttaat tttcctgact cttttctaag
    43441 taatccatct aattgttcgc cataaaaaga tgtttgtatt tcatttgcat aagtaggact
    43501 aacagttata attttatctg caaaatttat accagctttc ataaaattta ttgagccata
    43561 atactctata tctccattat caaagtattt ttgtggtaaa tccaatatat catataaaac
    43621 atgcttatca aatacacctt gatattggag attatgtata gtatacatag tttttatatt
    43681 tttatacccc tcaagggttg catatctttc ttttagaatt aatggtaaca tccctgtatg
    43741 ccaatcattt atatgtatta catctggata aaaatcaatt ctacttatag cttctaaaac
    43801 tgcatctgta aagaaagtat atctttctgc atcatctcca tatccataga tactctttct
    43861 ttcatcttct cttctaaaat aaaattcatt atctataaag taaaatttta caccatctaa
    43921 ttccatttct aaaagaccac agtattgatt tctccatcca acacgtacac taaacacagc
    43981 tatttctttt agttgatctt ttaaataact tggtatagtt gaatatttgg gcattattac
    44041 ccttacatct acaccttctt tttttaattc tttaggcaat gcataagaaa catctcctaa
    44101 tcctccagtt tttgcaaagg gccaacactc tgctgttaca taaaaaacct tcattagtca
    44161 ttcccccttc taaaaaattt atttatatat ttgtattctt ttccactaca agaggatatt
    44221 tttcatctcc tcttaattct ttcccatttg atacactcgt atttttatct aatattgcat
    44281 tatatagttt tgcatttgac tttattacac aattttgcat tactattgag ttctctataa
    44341 ctgttccttt ttcaacattt acctttctaa atatgatact atcttttaca gtaccttcta
    44401 ttaaacaacc agatgctata aagctatttt ctactttagc actatctgta taaattgtag
    44461 gtgattcatt cttttctttt gtaaatatct ttctttcaga atataataat tcatctgcaa
    44521 cttctaaatt taataaatct ttgctcattt caaaatatga ttcaacagaa tttacacatt
    44581 ttagatatcc tgtatattca taggctccta ttttcatgtt ttctgcttct tcaataataa
    44641 attcttctaa ataacaataa ttgcctttat ttacaatgtt atatatacat tcaacaaaat
    44701 cttcacgctt cataatatat gtctccatac atatgtttgc tctagggaac ttacctaaat
    44761 tatttcctac atttattacc ctattgttac tatctaaatt tagagttaag ttaccagcaa
    44821 aatctttatt tgcattattt acatttttat atattatagt tatatcatta tgagattttt
    44881 tatggtattc caatgcttga gaataattta aattgcatag catatagctt ggagctatta
    44941 aaatatattc ttcttcactc ttttctatat aatctagatt agctaagata gtatatatat
    45001 caccttctct atgtgagtaa gtatttctat acttagtgtt ttctggacta aaaattgata
    45061 ggccaccttt ttttctactt aaatcccaat ctttaccact acctatatgg tctgttaatg
    45121 atctgtattt ttcttttgcg aatattccaa ctttagtgat tcctgaattt atcatattag
    45181 ataaagcaaa gtctattatt ctatatcttc cagctattgg tgtagatgct attggtcttc
    45241 catagttaag tttgtttatt gctgggtctc cttttttgtt taaatttatt atccctaaac
    45301 attcatttct catcttaaag acctccttct attaatacgc atctaggttc gatattttca
    45361 tactcagaaa ctacatttat ttcattattt acattcttta tctgaacatt atcatttata
    45421 gttgcacact cacctatcat agctttttct atagttacat tttcacctat tacaacattt
    45481 ggcattatta ctgaatcaat tactttactt cctttaccaa caacaactcc atgtgataat
    45541 acagagttac ctacttcacc taaaattcta caaccatcag ctatcattga tctatgaaca
    45601 tttgcatttt tacctatata ttgtggaggc attgccatag tatttgtata tatcttccac
    45661 ttagggtctg ctaaatcaag tgtttctgga gattttatta tatccatatt agcatcccat
    45721 agactttgta tagttcctac atctctccag tatcctttaa atggataagc atacattcca
    45781 acattatctt ctaacatttt aggtattaaa ttttttccaa aatcatcata atttatttct
    45841 ggattttttt ctgcttcctt aaaataattt ctcaacattt tccaatcaaa tatgtatact
    45901 cccatagatg ccaaattgct cttaggttca cttggttttt cttcaaattc atagatactt
    45961 gaatcttcat ttgtattcat tatcccaaat cttgaagctt catcccaagg aacttcaatc
    46021 actgctattg tagctttaga gcctttttcc ttatgaaatt ttaacatttt gctataatcc
    46081 atcttgtata tatggtcacc agataatatc aatacatatt ctggattata ggtatctaca
    46141 aagtccatat tttgatatat cgcatgtgct gtaccattat accaatttcc ttctttttca
    46201 ttcataaatg gttgtaaaac atagacacca ccatttattc tgtccaaatc ccaatgactt
    46261 cccatgccta tatgtgaatt taatatcaac ggtctatatt gagttaaaac acctacagta
    46321 tcaataccag agtttgaaca attgcttaac acaaaatcta taattctata cttacctcca
    46381 aatgatacag ctggtttggc aattcttttt gtaaaaacac ctaatcttga accttgccct
    46441 cctgccaaaa tcatagctaa catctctttt ttcatttaat gacactcccc tcatctagta
    46501 attaatattt ataattgcat ttttactttt ataaacttta tcagtcaata aatgtcgtgt
    46561 ataaatgttg cttaaataat accttaataa aaataaaata atatcaacta tacaattgat
    46621 ataatgtata ccccataatg gacttaccga cgtatacatt attttccata agcactatag
    46681 tatttattaa tattcaatta tcggttaaaa aataatacaa attttatttt ataaatataa
    46741 aaacattttt tataaatcct ttgtgtcata tgaacatgct tattttttaa tttgttaaat
    46801 tcgtaatagc aaaatataat ctaatatttg ttaaataatt atccaaagaa aatcaagtaa
    46861 ttattaatca actttatttt ttaattgata cttctttgtc tataattta
//
LOCUS       DAEMHZ010000013        44371 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000013 DAEMHZ010000000
VERSION     DAEMHZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.013"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(189..794)
                     /locus_tag="KPK72_000657"
     CDS             complement(189..794)
                     /locus_tag="KPK72_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2487901.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(919..1036)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1096..2262)
                     /locus_tag="KPK72_000658"
     CDS             complement(1096..2262)
                     /locus_tag="KPK72_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2487902.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2410..4551)
                     /locus_tag="KPK72_000659"
     CDS             complement(2410..4551)
                     /locus_tag="KPK72_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG2487903.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4695..5802)
                     /gene="prfB"
                     /locus_tag="KPK72_000660"
     CDS             complement(join(4695..5726,5728..5802))
                     /gene="prfB"
                     /locus_tag="KPK72_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG2487904.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5817..8492)
                     /gene="secA"
                     /locus_tag="KPK72_000661"
     CDS             complement(5817..8492)
                     /gene="secA"
                     /locus_tag="KPK72_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2487905.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8624..9175)
                     /gene="raiA"
                     /locus_tag="KPK72_000662"
     CDS             complement(8624..9175)
                     /gene="raiA"
                     /locus_tag="KPK72_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2487906.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9728..10402)
                     /locus_tag="KPK72_000663"
     CDS             complement(9728..10402)
                     /locus_tag="KPK72_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG2487907.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10405..11457)
                     /locus_tag="KPK72_000664"
     CDS             complement(10405..11457)
                     /locus_tag="KPK72_000664"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG2487908.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11506..12465)
                     /locus_tag="KPK72_000665"
     CDS             complement(11506..12465)
                     /locus_tag="KPK72_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG2487909.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12561..13079)
                     /locus_tag="KPK72_000666"
     CDS             complement(12561..13079)
                     /locus_tag="KPK72_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG2487910.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13090..14424)
                     /gene="celB"
                     /locus_tag="KPK72_000667"
     CDS             complement(13090..14424)
                     /gene="celB"
                     /locus_tag="KPK72_000667"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG2487911.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14463..14789)
                     /locus_tag="KPK72_000668"
     CDS             complement(14463..14789)
                     /locus_tag="KPK72_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2487912.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14813..15121)
                     /locus_tag="KPK72_000669"
     CDS             complement(14813..15121)
                     /locus_tag="KPK72_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2487913.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15262..17175)
                     /locus_tag="KPK72_000670"
     CDS             complement(15262..17175)
                     /locus_tag="KPK72_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2487914.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17507..18289)
                     /locus_tag="KPK72_000671"
     CDS             complement(17507..18289)
                     /locus_tag="KPK72_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG2487915.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18292..20511)
                     /locus_tag="KPK72_000672"
     CDS             complement(18292..20511)
                     /locus_tag="KPK72_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG2487916.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20775..21872)
                     /locus_tag="KPK72_000673"
     CDS             complement(20775..21872)
                     /locus_tag="KPK72_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487917.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22187..23380)
                     /locus_tag="KPK72_000674"
     CDS             complement(22187..23380)
                     /locus_tag="KPK72_000674"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG2487918.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23517..23620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23809..24318
                     /gene="yyaC"
                     /locus_tag="KPK72_000675"
     CDS             23809..24318
                     /gene="yyaC"
                     /locus_tag="KPK72_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG2487919.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24429..24857)
                     /gene="fabZ"
                     /locus_tag="KPK72_000676"
     CDS             complement(24429..24857)
                     /gene="fabZ"
                     /locus_tag="KPK72_000676"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG2487920.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24873..25892)
                     /locus_tag="KPK72_000677"
     CDS             complement(24873..25892)
                     /locus_tag="KPK72_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2487921.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26089..26349)
                     /gene="spoIIID"
                     /locus_tag="KPK72_000678"
     CDS             complement(26089..26349)
                     /gene="spoIIID"
                     /locus_tag="KPK72_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG2487922.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26447..27115)
                     /locus_tag="KPK72_000679"
     CDS             complement(26447..27115)
                     /locus_tag="KPK72_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG2487923.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27181..28245)
                     /gene="spoIID"
                     /locus_tag="KPK72_000680"
     CDS             complement(27181..28245)
                     /gene="spoIID"
                     /locus_tag="KPK72_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG2487924.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28332..29585)
                     /gene="murA"
                     /locus_tag="KPK72_000681"
     CDS             complement(28332..29585)
                     /gene="murA"
                     /locus_tag="KPK72_000681"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG2487925.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29606..30343)
                     /locus_tag="KPK72_000682"
     CDS             complement(29606..30343)
                     /locus_tag="KPK72_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG2487926.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30792..32087)
                     /locus_tag="KPK72_000683"
     CDS             complement(30792..32087)
                     /locus_tag="KPK72_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG2487927.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32538..34370)
                     /gene="glmS"
                     /locus_tag="KPK72_000684"
     CDS             complement(32538..34370)
                     /gene="glmS"
                     /locus_tag="KPK72_000684"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG2487928.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34637..35983)
                     /locus_tag="KPK72_000685"
     CDS             complement(34637..35983)
                     /locus_tag="KPK72_000685"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG2487929.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36153..36710)
                     /locus_tag="KPK72_000686"
     CDS             complement(36153..36710)
                     /locus_tag="KPK72_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2487930.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36711..37463)
                     /locus_tag="KPK72_000687"
     CDS             complement(36711..37463)
                     /locus_tag="KPK72_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2487931.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37463..38542)
                     /locus_tag="KPK72_000688"
     CDS             complement(37463..38542)
                     /locus_tag="KPK72_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2487932.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38571..38786)
                     /locus_tag="KPK72_000689"
     CDS             complement(38571..38786)
                     /locus_tag="KPK72_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2487933.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38829..39506)
                     /locus_tag="KPK72_000690"
     CDS             complement(38829..39506)
                     /locus_tag="KPK72_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2487934.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39496..40575)
                     /gene="buk"
                     /locus_tag="KPK72_000691"
     CDS             complement(39496..40575)
                     /gene="buk"
                     /locus_tag="KPK72_000691"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2487935.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40597..41499)
                     /gene="ptb"
                     /locus_tag="KPK72_000692"
     CDS             complement(40597..41499)
                     /gene="ptb"
                     /locus_tag="KPK72_000692"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG2487936.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41803..42984)
                     /locus_tag="KPK72_000693"
     CDS             complement(41803..42984)
                     /locus_tag="KPK72_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487937.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(42981..43820)
                     /locus_tag="KPK72_000694"
     CDS             complement(42981..43820)
                     /locus_tag="KPK72_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG2487938.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        13210 a         8455 c         4730 g        17976 t
ORIGIN      
        1 tagttaatcg ttttaattct tcctttattt taaaatttaa atttgtctaa atatttctta
       61 ttttatatac attttttgat aaattaataa agccataata tatttaatat atcaatcaca
      121 atataggact gaagttataa ataaattatg gctattcaat acatttaatt ttatattttc
      181 ttgtctaatt atttctttat tatatttccc atttttttat aaagtggcaa tgttactaaa
      241 gatattataa cagccttcag cacattaaat ggagctatca tccaaattac aaaagtgaat
      301 aaatcatgaa cttgaggatt tatagctgaa cctaaaccta ttatagcatc tagtcccata
      361 agttgaccat aaagtggcaa cattacaaaa taatttacta atgaaccaat tatagtcata
      421 gatattgttc ctaatacaaa accaatcagt acacctttta aatctccact tctcttatat
      481 gaataacaaa gtatcattac ataagaacca ccaattaata tatttgcaaa ttctcctatt
      541 cctccagttg ttgttgctgt tattgcttgt aacacatttt ttactacaac tattccaaat
      601 ccaactaatg gtcctagaga cattcctccg aatattgctg gaatatctga taagtcaatt
      661 tttaagaaat ctggaaatat acctggtatt ggtgcttgaa taaacattaa taaataagat
      721 attgcagata aaattgacat tactactaaa gttcttgttg aaatcatatt gcctcttttt
      781 gctgtgtttt gcataattat tttcccccta atcaatatgt ttatattttt taggaaaaac
      841 aaaaaactcg aagaccctat cttcgagttt ttatggatat agtttaaaca aactaaaata
      901 aacttattta tcttccataa atatcttctc tcatccagac tttactgtcg gctttggaat
      961 ctcaccaaat caaccaccta agtagttcgc gggctatacc gccggtaggg aatttcaccc
     1021 tgccccgaag atcttctttg attttttaat tttccttaca ctcttatatt agttaatttt
     1081 ttaataattg tcaagttatt ttttctcata cactactttg ccatttatta tagtgtaaag
     1141 tacattactt tgaatctcta gtggagaatt atcccaaatt actaggtctg catctttacc
     1201 aacttctata gagcctactc tatcttctat tcctaaagtc ttagctggat ttatcgtaat
     1261 tgattctatt gctttttctt ctttcatacc atgttttact gcaatacctg cacatattgg
     1321 aagatattga actggtatta ctggatggtc tgtcattata catacatcaa gacctgcatt
     1381 tgaaagtatt cctgctgtat taaatgtcaa atttctaagc tcaaatttag acctttctga
     1441 caaagatggc cctactatta caggaaaccc ttcttccact aattcatcta caataagatg
     1501 accttctgta caatggtcta atgtaagctt taaattaaat tcttttgcaa ttcttattgc
     1561 tgtaaacata tcatctgctc tatgagcatg tgccttaaaa ggaatttctc ttcttaaaac
     1621 tggtattaaa ctttccattt ttatatcata ttcaggtttt tcacagtctt catcatcatg
     1681 actctcatac atatcaatat cctctaaata ttcttctgct ttttttaggt tttctctaag
     1741 taaagcagct atagccattc tagtttgagg agatttatca tcctgaccat aacagctctt
     1801 tggattttct ccaaaagcaa ttttagatgc aactggattt tttattacca ttttatcaat
     1861 tctttttcca catgtcttta tagctataca ttgaccaccc ataacatttg cacttccagg
     1921 ggttgtacaa acagaagtta ttccaccttc aaaagcttct ttaaaagttc tatccattgg
     1981 attaattcca tctataggat ttaattgtgg tgttattggg tcagtctctt catttccatc
     2041 tgctccttca aaacctatac catcttccca taaacctaag tgagtatgag catctataaa
     2101 tccaggaaac acaagtttat tactagcatc tattacctct acatctaaag gagctatcaa
     2161 atcttctcca atttctatta tttttccttc atctataagt atatctccat gaattatccc
     2221 atttgttatg gtatttattt taccattttt tataaaaatc attcattttc ctcctgattt
     2281 tcaagactat cgttcttatt ttaatatatt ttcttactat atacttttac aaatttttaa
     2341 catttataca ctcgagtaat cttatttaca aatatagaat ctaagtacta taaccataca
     2401 taaacttatc tattttttca ttgataaggc aactcttttt ttgtttaagt caattccaat
     2461 gacttttaca tctactatat cacctactgt aacaatactc atagggtctt tcacaaaact
     2521 attactcatt tctgatttat gaactaatcc gtcatttttt attccaatat ctacaaatgc
     2581 accaaagtct acaacatttc ttattgtacc ttttaaagtc ataccctctt gtatatcttc
     2641 aatttttaag acatcagttc taagtatagg ttttatacct tcttctcttg ggtctcttcc
     2701 tggttttttg atttcagcta tgatatcttt taacgtaact tgtcctactt ccagtttttc
     2761 acttaattcc ctcaagccaa tttcttttat tttttcatct atttcaccta tattcttatt
     2821 ctttacatca tctaaagaat atccaattat ctcaatcatt tttttacata tatcataact
     2881 ttcaggatgc acacctgtat tatctagtgg atttttaccc tctaagattc tcataaatcc
     2941 agcacattga gtaaatgctt gaggtcctag tctttttact tttttcaatt gtgctcttga
     3001 cgtaaaatct ccattttctt ctctatatgc tattatattt tttgctatag ctttacttat
     3061 tcctgcaaca tgttctagta gggagtatga tgcagtattt aaatctactc caacactatt
     3121 tacagaatct tcaacaactc catctagtac ttcttctaat ctctttttgt ttaaatcatg
     3181 ctgatattga ccaacaccaa tacttttagg gtctattttt acaagctctg ctaatgggtc
     3241 ttgtaatctt ctagctatag atatagctcc tcttattgat acatttatat ctggatgttc
     3301 ctcatttgca agttcagatg ctgaataaac agatgctcct gcttcactaa ctatcacata
     3361 ctgcacttcg ctatctattt cttttatcat ttctgataca aaagtttctg attctctaga
     3421 tgcagtacca tttccaattg aaattatatc tatatcgtat ttttcaatca atccttttaa
     3481 aactttcttt gctccctcta catcattttg agggtctgtt ggatatactg ttgtataatc
     3541 tagtagttta ccatttttat ctactacagc tattttacat ccagttctaa atgcagggtc
     3601 aaatcccata actactttgt ctttaactgg tggttgaagc aataaacttt taacattctt
     3661 tccaaataca cttatagctc tttcttgagc tatttctgtt aaatgatttc ttatttctct
     3721 ttctattgat gggaaaatta atcttttata agaatcttct attgaactta cgatttcttc
     3781 tttatttttg aaatttttat catttacata ttcgtttatt atgtaattta aaactttatc
     3841 attatttatt tccaacttaa cctttaggaa gctttctttt tctcctctgt ttattgccaa
     3901 aactctatgt ggagccatac tttttactgc ttcgctataa tcataataca tatcataaac
     3961 cgatttctca tcagtagcac ttttgctaac tatcatacct tctcttaaag ctaattctct
     4021 tatatacttt ctaatcttag catcatctga gactagttct gcaattatat ctctagcacc
     4081 ttttagagca tcttcaatag aaagaacttc tttctcttca tcaagataat tctttgcctc
     4141 tatttctata ttatctaaat tattctctag tattgacaat gctaaatttt ctaatccttt
     4201 ttcttttgct attgttgctc tcgttctttt cttttgtttg tatggagcat atatatcttc
     4261 tacttcttgt aatgtttttg ctttttctat attttgtgtt atttcatctg ttaatttccc
     4321 ttgttcatct attagtctaa ctacatcatc ttttctactt tgaagatttc tcaaatacat
     4381 taatttttca taaaattctc ttagtgtaac atcactcatt tcgccagtca tttcttttct
     4441 atatctagct ataaatggta ttgtatttcc ttcatcaata agttttaatg tattatttat
     4501 ttgttcatct ctaagattaa actctttttt taagatttga tttatatcca atttattcac
     4561 tcctaatgtt agtttttcat aagtataaca aaacagaacg aaggctgagg gttatcgtcc
     4621 tcagcctaag tgctgtaagt gcatattaag cactaacctt cttatacata tatcttaatt
     4681 acagtgcata tttattaaac tattttattc atttttaagt attcattaat aaacaagtct
     4741 atatttccat tcattacact atctacattg ccaaactcag catttgttct atggtcttta
     4801 accaatttat atggttgaaa aacatatgac ctaatttgac taccccaagt tatttgtgag
     4861 tatttgcctt gtatatcttc tattttttcc ttttgctcta actcttttaa ttctatcaat
     4921 ttcgccatta aaagtctcat agctctgtcc ttatttagat gctgagacct ttcattttgg
     4981 cactgaacta ctacaccagt agggatatgt gttattctaa ctgctgaatc agtcgtattt
     5041 acatgttgtc ctccagctcc agaagctcta tatgtgtcta tctttaaatc tgatggatta
     5101 atttctactt ctatattttc atctaactct ggtgttacat ctatagatgc aaatgaagta
     5161 tgtctttttc ctgatgggtc aaatggagat attctcacta atctatgaac tcctttttca
     5221 ctctttaaat acccataagc atttgttcct tctacaagaa ttgtagctgt ctttatccct
     5281 gcttcagggt ctgatattat atctaaaagc ttaactttat aacccttact ttctgaccat
     5341 cttatataca ttctcaaaag catttgagcc caatcttgag catctaagcc accagtacca
     5401 gcatttatag ataatatagc attattttta tcatactctc catttagaag agtctttatt
     5461 ttcatttcag ataatttttc ttccattgat tctatggact tttctatatc tctttctaca
     5521 gaatcatcat cttcttctaa acctatttca attaatactt ctatatcttc taataagctt
     5581 tttaagcttt catattcttc taatgtctct tttaaagatt tattttcttg aagtactctt
     5641 tgagcgactt cattatcatt ccaaaagtct tgttgattca tcttttcttc attaatttgt
     5701 atatttttca tcaatgaagc tatgtcaaag agaaaccctc aattcttcta aatttgaagc
     5761 catctgctcc atactatgtc tatactcttg taactttaac atttaatcct ccgagattat
     5821 cttccacaac agtttttata ttttttacca cttccacaag ggcataagtc atttcttcca
     5881 accttttcat cttttttaac agttttactt gttggaagtg ttccatctga tggagaagat
     5941 agattttcaa catctacaac tgcttttctt tccactggag tttctattgt tatattaaat
     6001 aaatatctta cagtatcttc tttgatatgt ttattcattt catcaaacat atcaaaacct
     6061 tccattttat aagcaataac tgggtcttgc tgacctacag cacgaagacc aataccttgg
     6121 cgtaattggt ccatagcatc tatatggtct atccaatggt tatcaacagc ttgaagtaag
     6181 attactctct caacttctct cattctctca tagccaactt gttcttcttt tgaagtatat
     6241 atcttcatag ctatttcata aactttttct gttatttcaa ctggattaag tttttctatt
     6301 tctggtattt ctatgctacc ttttggcaag aacagattat acatatgttc tttgaatcct
     6361 tcttcatcaa atcctttatc ttgtgtataa agagtaactg cttcttctat tatagaatga
     6421 gtcattgatt gtatttgttc ttgtaaatct tcaccttcta atacacgttt tctctctgca
     6481 tatattattt ctctttgttt attcataaca tcatcatatt gaagtacatg ctttcttatt
     6541 ccaaagtttt taccttcgac tttcttttgt gctccttcaa tagacttaga taatattcta
     6601 tgttctatcg gcatgtcttc ttcaagacct attttatcta caataccaga tattctatca
     6661 ctaccaaaca atctcataag gtcatcatca agacctatat agaatcttga actccctggg
     6721 tcaccttgac gtccagcacg acctctcaac tggttatcta ttcttcgaga ttcgtgtctt
     6781 tctgtaccta ttatagcaag tccacctgct tctacaactt gtttttgttc ttcttgtgtt
     6841 tgttgcttaa acttttcaaa tagtttttca tactcttctc tagcttcaaa tagaatttca
     6901 ttacctttta caggtatacc ttctattgga gtatctactc tatttactat ttcttcttta
     6961 aatccatttc ttctcatttc tctcttagtt aaaaagtctg gatttccacc tagaactata
     7021 tctgtaccac gaccagccat attagtagct attgtaacag ctccaagtct accagcttgt
     7081 gctattattt ctgcctcttt atcatgatgc ttagcattta atacctcgtg ctttatgccc
     7141 tttttcttta gtatttgtga taataattct gatttttcta ttgatactgt acctacaagt
     7201 attggttgat tgactttgtg cctttcaatt atttcttgtg ctacagcatt aaatttacct
     7261 atttcacttt tgtatacaca atctggtaag tcttccctta tcataagctt attagtaggt
     7321 acttggaaaa catccatttt gtagattgct ttaaactctt cttcttctgt ttttgctgta
     7381 ccagtcatac cagaaagttt tttgtacatt ctaaagtaat tttggaatgt tacagtagct
     7441 agtgttttag attctctttg tattttaaga ccttctttag cctctattgc ttgatgtaaa
     7501 ccttctgaat atcttctacc aaacataagt ctacctgtga actcatctac tattactatt
     7561 tctccatctt tagcaacata atcaacatct ttcttcatta ttacatgtgc tcttaaagct
     7621 tgatttatat gatggtataa ttctgtatga gatatatctg ttatattatc aacattaaaa
     7681 tacacttctg ctttttgtat acccgatgct gttagagata cagccttatc tttttcttct
     7741 aattcataat catctggttt taaggttaaa acaaatgtgt ttgcatctga atataagtga
     7801 gttgatttgt ctccaggtcc agatatgata agtggtgttc tagcctcatc tattagtata
     7861 gagtcaacct catccactat agcataattt aatcctcttt gaactctttg ctctttgtgt
     7921 attaccatat tgtcctttag atagtcaaat ccatattcat tatttgtacc ataagttata
     7981 tcacaatcat attgttcttt tctaactttt ggattttgac catgtataat aacgcctaca
     8041 ctcattccta aaaactcata gatttttgcc atttggtctc tatcacgttt tgcaaggtaa
     8101 tcatttactg ttacaacatg tacacctttt ccagtaagcg cattcaaata aacaggagca
     8161 gttgcaacca aagttttacc ttcacctgtt ttcatttctg ctattcttcc ttgatgtagt
     8221 actatacctc caatcatttg aactctgtag tgtctaagtc ctaacactct cttagaaact
     8281 tctctaacta ctgcaaaagc ttctggaagt atatcatcta tactttcacc atttgctaat
     8341 ctttctttaa atatatttgt catattttta agttccgagt ctgccataga ttcaaattta
     8401 ggttctaatg aatctattat atcaacagtt ttattgaact tttttaattc ttttttatct
     8461 gccatgttga ataaattatc cataaagctc atattttaca tctctcctcg ttctttattg
     8521 tactattatt cattttacca taaaaactat acttttaata ttaatttaaa aagctatcga
     8581 taatttattt atcgatagct aatatgatta aatctaaatt gctttattca tgttctatta
     8641 taccatatcc accattatta cgcttataaa ctactgctat ttcatctgaa tctatgttct
     8701 tgaacatata aaaatcatgt cctattaatt ccatctgtaa tacagcttct tcttcactca
     8761 ttggcttaac actaaatttc ttacgtcttt gaattactaa ttcttgattt tcatccccat
     8821 aatcctcttg ttcatcagct agatttatct ctgcatcttg gaatcttata ctcttgttgt
     8881 ctttatgttt atcttgtaat ctctttttat atttttttaa ttgtttactt aatttatcat
     8941 atacaacatc tatagctgtg tataaatttt cctgcatatc ttctgctcta attataggac
     9001 catttacagt agctattgtt acttctattt tttgtcttgc ttttctcgca cttactgtaa
     9061 cttttacatc agtgtctgga tgaatgtaat tatctaactt acttagttta gtttcgattt
     9121 tattttttat tgcatcagtt aattcgattt gttttccaga tataattatg ttcataaata
     9181 catctccctt caattatgcc agtataagaa aatttatact ttaataatat attctacacg
     9241 aactctcata atcctctttt tattatttac ttttatgatt ttttctattc aattgataga
     9301 attatttatc atttttagtt acattgatac ttatgtggat taatattatt acaaacacct
     9361 gtggataatg tggataaaac tgttaataac tcttgttttt gtttatttaa agctattttt
     9421 actcctattt ctatccacaa aaatctccat taattgttta ttaatatgtg aattcatgtc
     9481 ataagatggt aattttagga aattatgtgt attatatgcc aatatacctt tataattttt
     9541 taataaatat aaaccctcta aaacctccat aatctatctt ttctttcatt atttatattt
     9601 aaaactactt aaaagttata aagcatactc aacaataatt tcatatttaa tacctattaa
     9661 cattcatata cccaaattat aattaactta ctctctttat ttaaactaag tattttatat
     9721 tcatatacta tcttgaaatt atatttacga gttgtttaag cttttgtcta tctatatctt
     9781 caaaaggact gttatttatt ctaatcgctg taccaaaatg aaaattactt gccttagtta
     9841 attcctttat cttttctata ttattaaaat taagacctcc acctaaaaga atttttatgt
     9901 gattagaact aagcatcata ttctttatca tttgtatgtt atcaactata cttcctttcc
     9961 ctcctgatgt taatatattc gtaattttat cataatcctt caaaatttta acactatcta
    10021 ttgtatttga ttcatctatg gctctatgaa atgtaacatc aagattatca caacatttca
    10081 ataaaacatt tagatttttc tcatctatat tattgttttt atccaataca ccaaatacaa
    10141 ctccattagc accaatctct ttaaccacac ctatatcttt ttgcattata cttatatctt
    10201 ctttactgta tacaaaactt ttcgcatgat gtcttatcat tacgttcaca ggtatcttta
    10261 cactgttaac aacactttct ataagaccaa aacttggtgt caatccacct tcagttaaag
    10321 cactaactaa ctctattcta tctgcaccac aatcttctat aattttagcg tcttctactg
    10381 acattcctat tatttctaac ataactactc ctttgctaat tcatatattg catttgcaaa
    10441 tatctttgca tttaatatta tatcttctat atccatgtat tcatcaggat tatgagctat
    10501 tcctttttgt cctggaaatg aaggtccaaa cggaactata tttggcatta cttttgcata
    10561 cgtacctcca ttagttgtta cgggagttcc atctaatgat gtaacccttt cataagctag
    10621 ctgaagcttt ttaacaagat tactctcttt atcaaaatat acaggattaa aattagaaat
    10681 tacctctata tcgatactat ttggaaaact agcttttatt tcatccaata catctttcaa
    10741 tttataacta aaatcgtatt ttacctttat atctaatata gtatcactat tttctatatt
    10801 cagctcttta tatgtaacaa gcacattttc agacatattt tctataaaac ctaggtatac
    10861 atcttcttca taattgattt tactttttat tctaatcttt gccatgcctt tttcaccata
    10921 tacaacagga tacttacaat ctggtgtaaa tcccataata ggtgcttttt ctttttctaa
    10981 ataatatggt atatcttcaa atccactttc ttcatttgta ccaaatatta ttctaactct
    11041 cttatctaat tttaagttta attctttaat agcatataat ccatataagg cagacattat
    11101 aggtccttta ttgtctagta ctcctcgtcc ataaattctt ccattagttt cttctccctt
    11161 ataaggctga tgcttccaac catctccttc atgtacaaca tctacatgtc caattacaca
    11221 aacataatca tcactatctc catgctctgc ataaccaata taattatcca agtttctaac
    11281 cttaaatccc aatttttctg atatttctaa tgctttatta agtgcctccc ctactttttc
    11341 gccaaaaggg aagccatttt ctgactcccc tttgacacta ggtattttta caatatctat
    11401 aatatcactt aataaatctt cttttaactc atctatttta ttacttatga tattcataaa
    11461 aatctcctta ttataaaact ctattttaat tatgtaacca ctactttact cacttacttc
    11521 tattggtgct tttattctag cctcatatgc ttccatccac tcatttgata taaccttatg
    11581 tatagtcttt ccatctactc tatttgaaat atatacagta ggagattctt ctttagttac
    11641 tactgaaaaa gtaaaatcag ctatatttgt agctactccc cattcacctt tattattcat
    11701 ggcaactaat gataaatcac cagcttttcc tcttcttttg ataagctgtt tatcaagttc
    11761 atttactgca atatcacatg cttcttgtgg atgcttccct tctttcataa gtctaactat
    11821 ctcataagat atacatccct tcattaaatc ttctcccaaa ccagtagctg tagctcctcc
    11881 tatactacta tcaacataga atcctgaacc agatagtgca gaatctccta ttctacctct
    11941 tttcttcata aacaatccac ttgttgatgt agctgcacac atttttccag ttttgtctaa
    12001 agatatcata ccaacagtat catgaccagc atatgggctt aatcctttgt ctaaagtctc
    12061 tttttttctc tttttataat gtaacttcgc tctatcagtc aacatatttt ttctttcaaa
    12121 tccattttta tgagcataat cttcagcacc tattccaact aaaaaattat ttaccttctc
    12181 ataacttaac ttcctagcta tgctgacagg attagaataa tctcttatac cagcaactgc
    12241 gccaatatct aatgtgttac catccataaa tgcagcgtca agttctacct cacaattttc
    12301 atttggaagt ccaccatatc caaccgattt atagaatgga tagtcttcaa ctcttcttac
    12361 agcaagctct acagcatcac ctgcttccat gcctttttct aaatgttctg atgcctcagt
    12421 tattccttct agggccattc tccatgtcgc tatcattccc cacatgttaa aattcccctc
    12481 cttaaattat atagatttta ctaatgtatg cttattataa atagtataat atttaaactt
    12541 gctaataatt aatactctta ttaataacaa aattcttctt tcatcaaagt tatatcttct
    12601 ttatttgcta ttgcttttgc caatttacat atgttattta atgaatcatt aagaccaatt
    12661 ccaccttttt tgggagtctt aactatacat atgtcatttt tatattcgtt tatcacatct
    12721 atattttctt ctgacatagc tgcaaaagct ggtatatctg ttttatcatt tatattttta
    12781 gacagtattg ctgacatctt agaaaagttt tcgtaattaa agcttggtcc acagattact
    12841 acatcagggt ttaatttttt taccatagct atcatcttac tcgttacttc ttcttgatta
    12901 tttacaaaaa attcatcccc acaaaataag gtagctatta ccttagattc atttaaaaat
    12961 tgctccatca ttacacctgg tcctaatggg cttgattttc cagcaggagg tatattactt
    13021 ttctcttttc ctccagctcc agcttgtatt tggtccaata tcataactat tttcttcata
    13081 atcttcctcc tataatacta aatcatcaaa agatatatca tcatcttcct cttcaacagt
    13141 ttcattttct tctctcaagt attgcttatc tagcattttt ataaatggta tatacataaa
    13201 tattcctagt ataagaagta ctaattgtaa gactgcacct tgccatcctg ttgttaaaaa
    13261 accagagaat ataattggag ttgtccacgg taactgaact ccatttgtaa gaggcactaa
    13321 tcctgctgac atacagaagt aagctattat tatatttatt gttggaacta ttgcaaatgg
    13381 tatcaatagt attgggttaa gcattattgg tagaccaaat atcaatggct catttatacc
    13441 aaatattcct ggtaaaattg atagttttcc cattgacttt atacgtttac ttttacatat
    13501 taataacata gctatgacta atgatagtgt actacctgct ccaccaaatg tcgcaaacat
    13561 atcttggaat tgacctgtta ttatgtttgg tatttttgaa ccagcttgaa aagcagctaa
    13621 gttttcagct gatagaattt ttaatattgg attatacaca gccccaacaa cactacttcc
    13681 atttattcca aagaaccaga ataaatgtaa gaatatataa gcgattgcca tagcacctaa
    13741 actatttcct aattttaata atggcatttg taaaaactta aatataaatt catgtatact
    13801 tccatatggt gtagcttcaa aagctatctt aactaaaaag aaaactacca taactattgc
    13861 acttggtatt aatgctgcaa atgattttga tacagttgga ggaactccat ctggcatttt
    13921 caatgtccat ccttttttta caactgcagc aaataatttt acagaggtta ttgctgttat
    13981 catacctaca aacattccct tagaacccat ccaacctagt ggtatacctg ttaccatgta
    14041 aactgattct gttccttctg gtgtatatgg tattacaaat ggtgttatta taaaaaaggc
    14101 aactattgca actgctgctg atgctattgg gtctacatct atttgctttg cataagaata
    14161 tccaatccct aaaactgcaa gtagagtcat tacttcaaat gttgctacag ttggttgttg
    14221 caattttaca gcccaatctg gaccaaaaat ttgacctaca aattcattcc atccttttat
    14281 aggaaaattt gctactaata aaaataatga acctattatt aataatggtg atgatactaa
    14341 aaaaccatct ctaattgcaa ttaataattt atttttacct attttttcag ccaatggcat
    14401 aagtattctt tccaatctat tcatctatcc accctcattt cctattattt tttactataa
    14461 ttctacgctt gtttagattt tattagcttt atagcaagtt ttaatacttt ttccccattc
    14521 atagttccat aatcaacttc attgattata tctataagtt ttccttgagc ttcaaacttt
    14581 ggttttaact cctttagcat gtatttaact tgtggtccta ataatataca atctggattt
    14641 ttttcttcaa ctattttgtc aatttctgct atcggaaatg cctctacttc tattggtaaa
    14701 ttgtgttcat ctgctacctt ctgcatttta tctgcaagta aacttgttga cataccaaag
    14761 ctacaaaata aatatacttt tcttttcatt ttttaaatcc cccttaattt ttttatttta
    14821 atttttgaat ttcttttctt aacataatca tttcttttat aaggtttctc tcactcatac
    14881 aagtcattaa atggtcttga gagtgaatca ataaaataga tatatctgat ttatctcccc
    14941 cagcttcctt ttgtataagt tcagtttgta ctttatgtgc ttttaaaatt tcttcatttg
    15001 cattttcaat taattcatct gcttttttaa aatcgttttc ctttgcatag tcaagtgctt
    15061 cataaagcat agattttgca tttccagcat gaattataat ctttaaaact atttcttcca
    15121 ttttttcctc ctaagttgct tctctacttt tagattattt ataccttaca aacatataat
    15181 atcttataaa aacaaaaaaa catgcacaac tttttccatc acatggaaaa ttttgtgctt
    15241 atccatagtt cactatgaat tttatataag aatatctttt accttttcaa tgtcacattt
    15301 acttagaata tctagcatat tttcatcctc tgttaaatta gatatttctt taaataattc
    15361 tctaaatact tttctatcat catctttttt atatgctaac aaaagtataa tctttatatc
    15421 ctttccattt ctaattttta ttggtgtttt taaccttgca aaacataaaa cagattgttt
    15481 tatatatttc atgtctccat gagggaataa tattcctttt ccaacattgg ttggatatga
    15541 cttttctctt gttataatag attgtaaata ctctttttct gcataatgtt tttcaattaa
    15601 taaattagtt aaatatgtta tagcttcata aatatcatca tgttctaact ctcttattaa
    15661 ataatcagac atcattaatc tgttcataag tttatatgaa gtgtcttttt cttttatttt
    15721 gtttgtgaac tctttaagtt tattctcatc ctcatcagta aataatggtg ttgttataaa
    15781 aattggtttt tggatatttt catactcttg aaatgcaatt ataaaatcta tagtcttatt
    15841 aaagttatct ctttttaaat cttcctctct taatgtttca ataattttta tgttatcaaa
    15901 tctcttttct atctttactt ttaatagcat acttactcca aaactaaatg gacatacaat
    15961 acttgccttt ttatttgatt cccttttgct gtattttctt tctagagagg tttgaaagtg
    16021 aagtgtaaga taaccaattt catcttccgt tatttgtttt ccttttaatt caatttctat
    16081 tgaacttgat attactgtaa ataaaaaaca aaatttagtt tttatgttat ctaaaagtgg
    16141 attttctaag taaacattgc ttttcatttg atgcattgta acattcaaat gacatattaa
    16201 ttgttcataa agtaatatat cattattaaa atttattcct gattcttctg atactaaatt
    16261 tataattttt tttgtataac ttgctaattc ttcatttaga tataaagatt tcttaatatt
    16321 attactattt atttgtttgt tcatacccaa tattaaagat tctataaaca atttttcttc
    16381 ataatttaat actatggata aattagattt caattcatct tctatatcca atataagcat
    16441 ttttagctta agctctttct tagtatttga tacttttaaa gaatttccca gccttattcg
    16501 tattatattt ataagcaaaa atagcatcaa ttgcttgaat gatatatctg taagtcttat
    16561 atccatatcc tcctctatag taagcattat gttttttaca attattatat ctactatatg
    16621 gaagtactct atttcatcta tgtttaatct gtatttatct atgtatttaa ataagaatga
    16681 tactaacatt attcttatgt ctttctcatc cccttcaata tatataccac tgcctttttt
    16741 agtcgagata tttaaattat aattatttaa tgctactgat aatttactta tttcttctct
    16801 gacaatattt ttatttatgt acaacttatt agataaatca tttaaagtaa cttttttatt
    16861 attacaaagt agtaaattta atatctcaac ttgcttatga agctcactat ttactctttt
    16921 aacttcacct cgcaacttct gatttaccag tattttgtct gttccatctc cactaaattt
    16981 aatcccaata ttaggttttc ttttgatata agcattacaa aaagtagata accattcatc
    17041 aatatatttg caatcatttc ttattgtcct ttctgaacac tcaaaatcat tggcaatctt
    17101 attaacattt ataaactctt cttttaatag caatgttgtt aataaatttt cttgtctttt
    17161 attcaaacat atcacctctt tatgactcaa cctaaattta ttatatataa aaaagagcta
    17221 tacataaagg tatgctagct ctcttttaat atgatattta gctgacctgg tttttgaccc
    17281 cacactcgcc tactggaggg ttcgcttggg ttcgccgcac tactactctc tctcgatttt
    17341 attatcggta ggtcttactt ctaagccgca ccatgatcat taacccaatt tttagtgtta
    17401 acttacgtgg atccctttgc ctgcgactgg cttggacttt caaccacaga cctaaatatc
    17461 acttattcat tttaatattt taaactaaaa ttgtcaatat ataagattat tccataatat
    17521 aattatcatt tgcttttgta agtagagtca atacaaaaac cttattagca cctgataact
    17581 ttaaaagttt agaaatttcg tttgttgtta gaccagtagt aaatatatca tctattaaaa
    17641 gtatattttt attatttatt aatttaacat tttctttaac aacaaataca ttttttaatt
    17701 cttcttttcg ttctttctta ttcaatttat atagcattct tgtatatttt tttctactta
    17761 tgcaatctaa taatggaata tttactattt tacttaaatt aaatgctatt ttttgtgcct
    17821 gattaaaacc tcttttattt agcctttttt tatgaagtgg aacaaataat atataatcaa
    17881 attttatgtt ttctaaatat aatttttctt tcattatttg agctatatac ttagccataa
    17941 aggttttttg gttatacttc agccctaaca ccatcttttt tgtcacatca ttgtattcaa
    18001 tacaagatat tgatttatca aaataaaagt ctttattaaa acaataacta cactcttcaa
    18061 taaattcttt ttcaattgaa tgacgtatta ttggttttcc acacttcatg caaccatctt
    18121 gtataaaatt catttcttta aaacaacttt tacatattga ataagtattt gttttcttta
    18181 tggatttatc acaaattatg caacttatat tctcaggata tataaaatcc aaacatttat
    18241 taatcatatt tttaaaattc ttaataaaat tatcattgca tattttcata tttattctat
    18301 caacaaccct tcttgtttaa atttatttag cttatatcct aaatttgaat acctttgatt
    18361 aactctatta ttttttatca tatattcaag atatttcaca tctccaacta atacaactaa
    18421 tttttttgct cttgttactg ctgtatataa taaattacgg ctaagtaaca taggaggagc
    18481 ccacgctatt ggcaatacta caactggaaa ttcacttccc tggcttttat gtattgttgt
    18541 acaaaaacta tggtcaattt catctaattc atcatatagg tatgatacaa ttttagtctg
    18601 gtcaaataat atataaatag ttttcttatc tttatctatg tgataaacat atcctatatc
    18661 tccattatag ataccttctc cactttcttt ttggtcttct gtttcccatt tttttgtata
    18721 attatttttt acttgcatta ccttatcgcc aactctaaat aatcttttag aaaaactttc
    18781 ttcaacttta aatttttctt ttttgttaag atacttctgt aactctatat tgagattagt
    18841 aactcccaat tcaccttttc tcattgaaga taaaacttgt atatctttta atttatcaac
    18901 tttataaaat ttaggtaatc tttcattaac caatcctatt atttcattta aaatttcttc
    18961 attagttgat tttcttataa aaaagaaatc tttacctttt gtatttagat aaagtggttc
    19021 tccattgttt atcttatggg catttacaat tatcatactt tcttgcgctt gtctaaatat
    19081 ttcatttaat ttaactacat ttataatatt agaattaatc atatccttaa gtacatttcc
    19141 agcacctaca gaaggtaatt ggtcactatc tccaactaaa attactcttg ttcctaactt
    19201 tatagctcta agtaaattgt acattaagat aatgtctacc attgatacct catcaacaat
    19261 tattacatct gaatttattg ggtcttcttc atccttaaaa aaagttaaat catcatctgt
    19321 tgaaaatccc atttctagca acctatgtat agtttttgct tctttattag aagtttcact
    19381 cattctcttt gctgctcttc ccgttggagc tgccaaagtc acacttttac cattattttc
    19441 aaatatatct atgatagcat taatggtagt tgttttacca gttcctggtc caccagttat
    19501 aattaatact ccactattaa cagattcttt gactgctaaa atttgttttt ctgctaactt
    19561 aattttctta tcttcttcta aaacattaat ctcgttatct atatctattt ttaagtcttc
    19621 aaattcatac tgtgataatt ttactatttg actacatact ccattttcag ctaaatagta
    19681 tggcataagg tatataagat tttcattgtt tactttctct atatgtattt tttgattata
    19741 aactagcatc attacacact cttttattat ttctccattt agctccaata atttttccga
    19801 atcttgtatt agaacatgct ctggtaaata tgtatgccca ttacttaaag atttatttaa
    19861 agtgtataag attccctgac aaattctatc tttagagttt ttatctatac caattttact
    19921 tgctatacta tcagctactt taaatccaat tcctcttata tcttcagcaa gttggtatgg
    19981 gtttttattt ataacttcta ttgcactgct tttatatttt ttatatattt ttaggcagta
    20041 atttggtgtt attccaaaag gagatagctg tattattata tttctcaatt ctctgtcttc
    20101 ttcataactt tttactattt gttttacttt tttactccct ataccttcta cttcttgtag
    20161 tttttctggt gaattttgta tcacctctaa cgtatctaca ccaaacttat caattattct
    20221 ttttgccatt ttttcaccaa taccatgtat cattccagaa gataagtaaa cataaattcc
    20281 ttctaaggat gatggtgtta ctggcataaa actattcact tcaaattgag tcccataaat
    20341 cttatgattt acccactttc cttcaacttc tatgctttct ccaattgcca aagtaggcat
    20401 acatcctaca accacaattt catcattttc atttaccagg tgagctattg tataaccatt
    20461 atcttcgttt ttaaagacta tttcacttat cattccttct aatttttcca tttttatctc
    20521 ctaaaaatac taataatcgc tccatttaaa tgttagtata attttatata ttaaaactat
    20581 gataatcgta gaaaagtttt atatttttat acttaacgta atttccaact attatacatt
    20641 ttatcagaaa caaaaaaaat ctgcatccat aaaatatggt accatgaaat acaggtacca
    20701 tatttaaata gctattttat tttaattgct ctatatttag agtataaatt aaattccata
    20761 agtatattac agtactaact tatttcagat ttattctcct tgtacccctc agttttagta
    20821 attcttctat aaagcataca tagtaaaatt aaaattccca tgtaacctgt atatggatat
    20881 agtgtaccta ctaacttatc aaatggcagt aaacctccta tacaagctaa tatacttacc
    20941 actattgtta taattttaaa cttagggtgg tcatcttcta caaatcggtt tgttactgac
    21001 cataacaatg gtacagctgt agtgtatatt cctagtaaaa gtactactga gaataaaata
    21061 cctattattg gagatatctt atcagctaag taaagtgctg gtatctcttt tgtgtatata
    21121 tttcctatgt ctgaaagtaa tgccaaattc atcattatag ctgctgccat aagtgctact
    21181 ccacctacta taccacccca tacagcatct ttcttattta ctgcagaagc tcccattcct
    21241 gtcaagaatg ttattactat tattacattg taacaagtat aaagtactcc cgagaaatat
    21301 ccatttgatg tagcagtttt aatatttaaa ctatcaacca tatttccagc atttgataaa
    21361 gcatcaatat tgctaaataa actaattgct cctactaaaa gtgtaaatat tattattata
    21421 ggtcctatat tacctagtat tttagaaagc cttgtcaaac ctaatgaaac agttattaga
    21481 gaaacgattg ccataccaat cctacctaca tatggattta atccatagta ctctgataat
    21541 gtagctcctg ctcctgcaat cattattact aatgttccaa ataaaaacaa tggaccaaac
    21601 cattcaaaaa atgttccaag atattttcca caatatactt gataaatttt tattggctcc
    21661 ttaagtttca attctcttcc cttatctata acttctgctc caaaccatga aaatagtacc
    21721 atagagatta ttccacctat aatacccgca tatccataaa acgtaaaaaa ttgcagtatt
    21781 tcttgaccag ttgcaaatcc agaacctatt acagtagcaa cataagctcc agcaaaactt
    21841 ataaccgttt taatatttac tttgcctgac atacactact ccccccaatt aaaattattt
    21901 ttaaagtgta agtttattaa gattatatta gactcattat tgtgatattt caatatattt
    21961 ttaattattt aacattagtt aacacattct taaccatcct ttaacatata tcccataata
    22021 tgtataaatt tagtagagta ttatataaaa tgtattgacc agattaaata ctgtttattc
    22081 attatatgat gataaattaa catttattaa aaatatttaa ctcataataa aaaagctgtt
    22141 agaaagtaat cataaaatta cattctaaca gctttttagt taaaaattat tcacctaaag
    22201 catcttttct taactgctca accttgtcag ttctttccca aggtaaatct atatcagttc
    22261 taccaaagtg accataagca gcaacctttt tataaagagg ctttcttaaa tctaagtctc
    22321 ttattatcgc tcccggtctt aaatcaaagt gtttatttac taattcaaca agtttttctt
    22381 ctgaaacctt gcctgtccca aaagtatcta taaatataga taatggtcta gctataccta
    22441 tagcatatgc aagttctatc tcacatttgt ctgcaagacc agccgcaaca atattttttg
    22501 caacatatct agctgcataa gcagcagatc tatcaacttt tgtagggtct tttcctgaga
    22561 aagctcctcc accatgtcta gaatatccac cataagtatc tattattatt tttcttcctg
    22621 taagacctgt atctccttgt ggtcctccta taacgaatct tcctgttgga tttatataaa
    22681 ttttagtctc ttcatcaagt aattctgctg gtataacttc cttaataaca tgattaatta
    22741 aatcttctct tattttatca tttgatactg tttcacaatg ttgagctgat ataagaactg
    22801 tatgtactct tacagcttta cttccttcat attcaacagt aacttgagtt tttccatctg
    22861 gtcttaaata atcaactaaa cctgtttttc taacttctgt aagtcttctt gataatttat
    22921 gtgccaaaga tatcggaagt ggcattaatt ctggagtctc attacatgca aatccaaaca
    22981 ttataccttg gtctccagct ccaacttttt ctatttcttc ttctatctct tctccagtct
    23041 tactttctag accttcatca actcccatag ctatatctcc agattgttca tcaatagaag
    23101 ttataactgc gcaagtatca caatcaaatc catattttgc tcttgtatat ccaatttctc
    23161 ttactgtttc tcttactaat ttaggaatgt ctacataagc agatgtactt atttctcctg
    23221 ctactaaaac taaaccagta gtaactgtag tttcacaagc tactctagat tgtgggtctt
    23281 tttctaataa tgcatctaat attgaatctg atatttggtc acatatctta tcaggatgtc
    23341 cttctgtaac tgattctgac gtaaataaat gtcttgccat ttttattccc ccttatttat
    23401 tcttaatttt tattataggt tttaccttat ttttaatttt agaaatttac tcaaaataaa
    23461 aaaacctctc ctttataaga agaggttaat aatcgtaatt acatactaat taactgacct
    23521 catctttcaa gacttttgtc ttgtaggaat tagcaccttt tcccatttgg gtggttgctg
    23581 ggcttcacag ggcctatccc tcagcctctc ttgataaggt aatcaagatt taagttttgt
    23641 tttactttac tagcttacta tttttttaaa attttgtcaa tatttttagt catatttttt
    23701 ctattccttt ttttgtaaga caaataaaat tttctatcta tctgaaattt aaagttaggt
    23761 ctttttaatg ctttttattc atatattttt ttgaaggggg tgttcctatt gtatttagct
    23821 aaagtaaact acagcgatga tgatgttata cagatgctaa gttcagttct aaaaactata
    23881 attaatgaaa atacgattgt tgtttgcata ggaacagaca gggctatagg tgatacttta
    23941 ggccctttag ttggaacaat acttaagaac agtaacttta aataccccgt ttacggtaca
    24001 ctagataacc ctattcacgc cttaaatata tatgagtctt tagatactat aaaaaataca
    24061 catatacaag gtaacttctt ggctatagat gcttgcttgg ggtcgcagag taacataggt
    24121 aatatacaga ttagagaagg acctatcctg cccggaaaag gtgttggtaa aaaactacct
    24181 caaataggaa attattctat agtaggaatt gtagataaaa ttgatgaaaa taataaactt
    24241 tcatttaata atatacgcct tagctttata ttagatttag ctgaaacaat agccttggct
    24301 ttattagtgt ccacctaatc taaatataaa ctaaatgata aaattcttta tataaaaagg
    24361 tagatatttt agagtatctg attaaatact ccaaattatc taccttttta cttttatatt
    24421 tcaagttttt atttttctac taaagcaaac attaattcac ctttacagac aagttcttca
    24481 cctacatatg ccttagcatc tgccttagct atattttttc taagagttgt catttcaact
    24541 tccatagtta atgtatctcc tggtcttact tctctcttga ttctaacttt atctatacct
    24601 gcaaatacac ctagttttcc tttgttttcc tctacagaca tcattgccac tccacatact
    24661 tgtgctagag cttctactat tagtactcct ggcatcaaag gatattctgg aaagtgacct
    24721 tggaaaaatg gctcattaac tgttacattc tttataccaa cagctctttt cccaacttct
    24781 agctctgtaa tcttgtctac taataaaaat ggatatctat gtggtattaa tttttttatt
    24841 tcatctatat ttaacattct aaactctcct aattattttc ttttgaaaga cccaccagtt
    24901 tcaagtaagt ctattgaacc taaagctatt cctgtacctt ttgcaacaca agataatggt
    24961 tcatctgcta ttgtaacagt tattcctgtc ctctgttcta ttattttatc taatccatat
    25021 aataatgaac ctcctccagt cattattata ccgacatctc ctatatctgc tgctaactca
    25081 ggaggcgttt tttccaaaac agcatgagta gcaactacta tttgctcaac acattctcta
    25141 agtgcttcca acatttctgt tgaatttata gttatagaac taggaagtcc tgtaattaaa
    25201 tttcttcctg ttatcttcat atatttttct tcttctctct taaatgcaga acctatttca
    25261 aacttgattt tctctgctgt tctttctcct ataagaagat tgtgttgttt tttcatatat
    25321 ttaactatag catcatcaaa tctatctcca ccaaccttta tagattcact tacaacagct
    25381 cctcctagag atattacagc tatatctaca gtacctccac caatatctat aatcatatta
    25441 ccattaggct gtgatatgtc tacaccagct cctatggcag ctgctattgg ttcttctatg
    25501 atatatactt ctctagctcc agcttgtctc gtagcatctt ctactgctct tttttccact
    25561 tctgtgacac ctgttggtac acataccata attcttggca taaaaaatct tccaaatccc
    25621 tttttatcaa ctatcttatc tataaaagat tttaacatct tctcagttac atcataatct
    25681 gaaattacac catctcttaa aggtcttata gcgactatat tacctggtgt tctccctatc
    25741 atctttttag cttcttctcc cacagctaaa acagaatctg ttcttttgtc tatcgccaca
    25801 acagatggtt cttctaaaac gattccctta ccactaacat aaacaagcac atttgcagta
    25861 cctaaatcta tgcctatatc agctccagcc attttttcaa ctcctttatt tataatatta
    25921 gattatatta taagcctatt ttaatactcc tataataaag catatacaaa aatctcgctt
    25981 taatcaagag aaaataaatc ttttgagagt attattattt tgctatttat aattacttaa
    26041 tatatttctt tttataattt aattcaataa aaaaagtagg tttttaacct acattttttt
    26101 atgttccttc tcatatttta atttcgttgc taatccccct ctaatatgtc tctctgattt
    26161 atttttgtcc agcacatttt taacttcttt ggcaagggat ggatttattt ccttcaatct
    26221 ttctgtaaca tccttatgaa ttgtactttt acttactcca aatgtcttgg cggtttgtct
    26281 tactgtagtg tttttttcaa gtatatattt tgctaccact atggcccttt cctctatatg
    26341 agatctcaaa gattaccccc ctcacataat agctttaatg atatatatgt aaaaacacca
    26401 aaaaatataa caagcttaac caaatatgat taaacttgtt atactattac ttaattagac
    26461 tcattgggtc tatagatttt tctccactat acgcctctaa atgcacatgt ataccttctt
    26521 cactttctat ttgagaagta ctacctacag tacctagaga ttgtccttct gttaccttct
    26581 gttctttctt aacactaaca tttttatcta gatttgaata tactactaca atgttattgt
    26641 cactttttat tttgactgat tgtccatatt cttcatcatc aaatacatct actactattc
    26701 cgtttaatag agattttact tctttgcctt tagtacaact aatatctaca cctttatgag
    26761 tttcccaaac atcaagtgtt ttagaataac taggctcttt ttctgaatat cctcttatta
    26821 ctttatctcc aatgtaattt aattttgttg tactcgattt attttcctct tttgacttag
    26881 ctttttctaa gttttgtttt gaatccgttg cagtagggat agcatctttc ttgtcttttt
    26941 caattaaatg tatttcctct tcactatctt tattagcatt ttccataatt cctttattag
    27001 aagccaattt atctacatta ttatttgtaa accaaactcc acctactgct aataaacaaa
    27061 cacatacaaa caaagataaa taaaaaccat ctttttctaa cagctttttc ttcattttgc
    27121 acctccaaat atatttctac atttggatta ttgacgtgtt tttggcattt tatacaatta
    27181 ttagtatata tcttttattt ttgtatctgt gtagtaatgt gataatatat cataatattt
    27241 ataaccctct tctgccattc cttctgctcc ccactggctc ataccaacac catgaccata
    27301 tccttttacc acgaaatcta tgtatccttc tccaaatttt atgtcaaagt ttgcagagtt
    27361 caatttaaaa acagttctaa tgtcttttcc agtcaattct ttatttccta attttatttt
    27421 ttctacagtt ccggcatcac ttcttttagt tattgaaact tgtttactta aattattcac
    27481 atctatcaca atagtgctat atgcccttct aagactttta acaaaatctg tgttacttat
    27541 tttaagtgtt gacgcaaatt taggggaata cttatcataa ggactttcca cagactttaa
    27601 ataaggatat tttgcagaaa atacttcttc actattttct gtttttcctg aagatgtaga
    27661 aaaataaaga ggaagtatcg ccttatcatt ataagttatt atttgacctt tagttcctct
    27721 aactgcttct tggattttgg aatatttatt cttaatccat tcttcaccat ttaatttttt
    27781 aagagtatca taactcttat attcttgaca atgtttataa tcagtacata ctactgcatt
    27841 tttatgttta ctagatttac catgttcctg tttatatact acatatgttc tagctgctac
    27901 agattgagct tttaaagctt ctatatcaaa ctctgaagac atctcaccag ctagtactcc
    27961 acataaatag ttctccatgt ccatcttttc ggttttccct gttatatgat tatatacatt
    28021 gataattggt gcttttttat ttacagtttc atagttcact gtattttttt cttctttatt
    28081 acctttgtct tcaatatcac tttttttaat agttttgttt atagatactg ggctctctgg
    28141 tttttcagtt aattctacat ttttataaga aactaaagtt ataagtgatg gaactaacac
    28201 agagcaagtc acaaatccta acaaaacaac caatgggttc ttcatatact ctcctcctta
    28261 gcttttaaaa ccttgtattt aagtatatga acaaccctat taatttataa ctttcttcta
    28321 taaaccaatt attaatcttc tactatttcg atttgaccac ctagtgctgt tatcttctta
    28381 tctatatcaa cataacctct ttggatatgg tatatttctc ctatttgagt ttctccttct
    28441 gctataagac cgcataaaat aagggctgcc cctgctctta aatcagtagc gtttactgct
    28501 gcaccgtgta gctcatctac accattaaca accgcacttc ttccatctat ttttatatta
    28561 gcacccattc tattaaattc agcaacatgc ataaatctat tttcaaaaac agtttctata
    28621 actacaccag ttccattggc tacagtaagc atagccataa attgtgcttg aacatctgta
    28681 ggaaatcctg gatgtggtaa agtttttata tctattggtt ttaatacttt tggacctaca
    28741 actcttacag aattgtccat ttctgttatt tcacaaccag cttctcttaa tttagctaca
    28801 actggtttta aatgctccat taaaacattt tcaacagtta tatcaccttt tgtcatagct
    28861 gctgcaacca tataagttgc tgcttctatt ctgtctggaa ttacattatg ttctgctcct
    28921 tttaactctt taacaccttt tatttttata gtattagtac ctgcaccttt aacatctgct
    28981 cccatttcgt tcaagaagtt tgctaaatct actatttctg gctcttctgc tgcattctct
    29041 attattgtag tcccttctgc taaagaagct gccatcatta tgttttcagt tgctccaaca
    29101 gatgggaaat ctaaatataa tttatttcct actaactttt ctgttgctgc ttcaacaaaa
    29161 ccatggtcca tttctatctt tgcacctaag gctttaaatc cttttaaatg taaatctata
    29221 ggtcttgttc ctatagcaca tcctcctggc atagaaattt tcgtactatt aaatctagca
    29281 agcaatggtc ccataaccaa aaaagaagct ctcatctttc ttacaagctc atatggagct
    29341 tcacaagttt ttatattgct tgcatcaaca gtaagtgtat tttctttata ttcaacttct
    29401 gctcctacat gtcttaataa atctgatata acatgaacat ctcttaaatt tggtactcca
    29461 tttaatgttg attttccgtt tgctaataaa gtcgctgcta ttattggtaa aactgcattt
    29521 tttgcaccat ctattttaac actaccttta agtggattac tcttttttac tattatctta
    29581 gccatatttt ttgatctcct cataattaat aatctaattt tataattggt gtggcaatat
    29641 atatgagtgt ttttccgtca tcctcattat atttaattcc tatatttaaa ttaatttttt
    29701 cccctaaata ttctaattca taatcattta atactttact atatgctgtt atagataaaa
    29761 agtttacgtc tttaacttca tctatttgct tagcactcat attatataat attttttgta
    29821 aaatatcatc atatttgttg ttttgtaacc tttttgtgta ttctccaaca atacaagcag
    29881 acacatctac ttgagatgaa tatttattta acacattttc tacgttacta taaataacct
    29941 ctttatgttt atatactttg ttatccaata tgtcaactac tatgtaactt tctttgttat
    30001 ttttatttat tccttttata gaaatttctt tatcgctacc ttttatagta gcacataatt
    30061 gtattttatc gccagaccct ttttgattcc aatttattcc ttttttatcc aactttagat
    30121 tgttaattat ttctataaaa atattatgaa attctttatt tgataaatta tgatttataa
    30181 cagaattaat tttaacatta taaaatttaa aatttgcttc tgatatttca agagctttac
    30241 ttaatctatt gtatctagta tttatacttg tatctgcata agatattgtc attcctataa
    30301 tcattaatat aaaaaagctt atagctaatt ttactttttt catagtaaaa aatccccctt
    30361 aaatatgttt ttctgtataa tagaattatt aacaaattta agagagatat actttctcaa
    30421 tatgtatttt tatacatttt tgtatttttc atctaataat ctctttcaaa ctcttcaatc
    30481 tcaattttct ctatccttct cctggacttg ccaatttctt atatgtatca aatacatctt
    30541 gcattaattc ttatatattc tcttctgtaa tataaattac ttagtctata cttaaatcta
    30601 taatctataa tctataatct ataatctatt catattttac caacaaaata aacagataat
    30661 acttttttat caatttccat agctccaaat gaacatacct aagtacactt cccaatacac
    30721 aatttacaca ctcttcctaa taaatcaaaa acacctttgt tttaaacaac tctattgcac
    30781 tttcaaaaac tttatttgtg ttctcccact tcaattaaat ttgaattatc aagactccag
    30841 cccatccatc catcactata aagggaggta ttttccactc ctatgacatt tgcatatgtt
    30901 aatacttcag cagcacgcca tccacttcca cacataaata taagctgttt attcacatct
    30961 atattttcct tgtcccacat ttctaaaatc tcatatttat ttctcattgt attatcaata
    31021 ttacgatatt cttctagtga aacactatca ctacctgaat gtccatataa agctcctggt
    31081 atacgccctt ttttatcata atatgtatat ccagatactt ttcctatatg ttcatcccaa
    31141 gtacggttat ctactagtat aaacttattt ttttctttta gtttttgtct taattcagat
    31201 gtagtaacaa ttaattgtga gttaactggt atatctgctc caaaattagt acatgaatgt
    31261 tttggattac taataaattc taattcatat cctgctgata accatgagtt tgttccacca
    31321 ttcaaaactc ttacatcttt tactcctata tatctcaata taacagctag tctataagat
    31381 gccataggtg tactacttga aactattaca gtatcatcct ttgttaatcc atagtctaaa
    31441 gcaaacttag ttaagttatc atcattatca agcatccatg ttggaggagg ttctataata
    31501 tcagtattta catgaacact tgtaggtata tgcccttttg tatacgattc ttctccccaa
    31561 ctcgcttcaa tcatttttat attctttgaa tcttcaaatg tctcaggaat ttttccatct
    31621 aatatatctt ttattataaa agcaggaact atcatttgat aatttttata tctttccatt
    31681 ggaagatttt cgtcgtctgc ccattgttta atatcatatt tatatagata tttatatcct
    31741 tttttagata aataatctgc tacaaccaaa gcatcttttc catttgcatc atacaatact
    31801 atatttttat ctaaatcaat tcttttagtt ttcaatgctt gttctaacac ttcatctttt
    31861 ctatctgagt atacacttaa ccaattagca gaaaaatcta ctgctccttt tatatgtcca
    31921 cctcttttta ctccatctaa cttccagcca ttataagcat catttaagcg agtatcaact
    31981 ataacccaag cgttatcttg cactttttct tgtaaaactt cagtagatat tgccttaaga
    32041 ttaacaaaat aatcttcttt cttttctttt ttaaaatcat ttgtcataat ttatactccc
    32101 aatttaaaaa aatttttatt tgaaattatt ttactaaaat aaagtaattt tcttatactc
    32161 tattagcttc attatattct tttataaata gttccttata ttcttatttt aaactactca
    32221 ctttatctta tcacaaggtt actaatacct tgtattttaa tttttctcat aagctattat
    32281 ttttttatct gatattgact atatgtttat taagtactca ctatatttta tatgaactat
    32341 atttatcgtt ccctatacct ggtatggtct atatatttat cattttacag atgatttaat
    32401 atcaatcttt ttataaaatt tattatagca aaaacaaaac aaatttaata tttatctaaa
    32461 aaactcatcc tataaaatac aaataacagg atgagttctt ttcaataaat attatctatt
    32521 taatttttaa tctgttttta ttcaactgtt actgatttag ctagatttct tggcttatct
    32581 acatcgcatc ctcttagtac agagacatga taagcaagta attgcattgg tacaactgct
    32641 aatattggtg ataaaatatc atctgaattt ggtatataga ttattttatc agcagctttc
    32701 tcaacatcct tattatggct ttgagctatt gctactacat aagctcctct tgctcttact
    32761 tcttccatat tagaaaccat tttttcaaat aacttttctt gagtagctat ggcaattaca
    32821 ggagttcctt tttctattaa agctattgtt ccatgcttta gctctcctgc tgcaaaagct
    32881 tctgcatgta tatatgaaat ctcttttaat ttcaatgaac cttccattgc taagctatag
    32941 tctatacctc ttcctaaata gaatgcatgt tcagaactaa caactgtttt agcaacttct
    33001 tttatatatt cttcattgtc tagtatttct tgtatttttg atggtatttc tttcatctta
    33061 gaaatcatac tatcataaaa ttctctagtt attgttcctt ttttgatagc aaagtctaaa
    33121 gctatcatgt ataaagaagt tatttgagta gtatatgcct tagttgatgc aactgctact
    33181 tcaggtcctg cccaagtata gaatacatca tctgattctc ttgctataga tgaacctaca
    33241 acgttagtta ttgaaagtat tcttgctcct ttagcctttg aatctcttaa tactgctaga
    33301 gtatctgctg tctcaccaga ttgactaact aatattacta atgaattttc atctacaaat
    33361 gggtcgctat atctaaattc tgatgctata tcagttatta ctggtatatt tacaaatttt
    33421 tctatggcat acttaccaag taatccagca ttatatgctg ttccacaagc cactatataa
    33481 actttattta ttttttctaa gtcttcttta gaaatattta tactgtctag aattatattt
    33541 ccattatcat caagtcttct ttctaaagtt tctcttactc ctgttggttg ctcatgaatt
    33601 tcttttgaca taaagtagtc atatccacct ttagacgcag cttctacatc ccaagttaca
    33661 tgaaatactt ctttttcaac aagatttcta ttagagtcat aaactgttac attttcgtct
    33721 tttagatgaa ctacttctcc attttctaag aaataaacat ctctagtata ttttaataat
    33781 gcaggtatat ctgatgctat aaagttttca ccttcaccaa caccaactac aagaggacta
    33841 tcttttctta ctgcaactaa ttcatttcca tgttctttac atataacacc taatgcataa
    33901 gctcctctta attttgatat agttttgtat acagcatcta ataaattacc ctcatagtat
    33961 ttatctacta aatgtgctat tacctctgta tctgtttgag attcaaactt aacaccttca
    34021 gaaattaatt cttcttttat ttccatatag ttttctatta tcccattatg aacaactgct
    34081 attgttttgg cttgattaaa atgaggatgt gagtttacat cagatggttc tccatgagtt
    34141 gcccatctcg tatgccctat acctaaattt ccatcaatag gattcttttc taagtcttct
    34201 gcaagtacag aaagtctacc tttaaacttt ctaatattaa gttctttttc attagaacta
    34261 ttaactgcta ctcctgctga gtcataacct ctgtactcta gcttagaaag cccttctact
    34321 ataacttctg ctgcctttct acttcctaaa tatccaacta ttccacacat tatttgtgtc
    34381 ctcctaaaaa tattatttat atattttaag agttaggtca tataagttta ctttggcttc
    34441 cctttgtcca taggattgct cctattattt aaagaagtct ttcgatggta aacacaccgc
    34501 agagcatccg ccgacaattc gattaactct gacctcgtca acttagttcc tatattactc
    34561 aataattatg tctataacta agttctggcg cttagaaagg tgtcctcagt atttatatct
    34621 gagaataccc tttaaattac gataattttt cttgtataag attagctaag tttgttgcta
    34681 attcttttat ttgaccttcc tctttaccct ctaacataac tctaactaat ggctcagttc
    34741 ctgatggtct tatcaaaact ctaccatttc cgtctaaaat agattctatt ctttctatct
    34801 cagtttttat ttctggatat tccatatatt tgttcttatt ttcatttttt attgttgcat
    34861 ttactaatac ttgtgggtat tggctcatta ttgatgcaat ttctgataaa ggtttctttt
    34921 cttgcaagat tatatttgca agtattagag aactaagcac tccatctcca gtagtgttgt
    34981 aatctaaaaa tatcatatgt ccagattgct ctccaccaag attatatcca tttttaacca
    35041 tatcttctaa aacatatctg tctccaactg ctgtagtaga taaatttatt ccattttcct
    35101 tagcagctat tgtcaaccct atattactca taactgtaac taccaaagta tcttgtgcca
    35161 atttattatt tttctttaaa tttaaggcac tcaatatcat tatatggtct ccatcaacaa
    35221 tttgaccctt ttcatttact gcaattaatc tatcagcatc tccatcatat gcaagaccta
    35281 agtcagcatt atgttccaaa acagcccttt gaagttgttc tggatgtgta gagccacatt
    35341 tatagtttat attatttcca tctggtgaac tatttattga aattacactt gctcctagtt
    35401 catcaaacac tattggtgcc actttatatg acgcaccatt agcacagtcc aaaactactt
    35461 ttaatccctt aaagtcagtg cttattattg attttaggta gtctacatag tctctttgtg
    35521 cacaatgttc atgtaatttt cttcctacat tttcaccaat tggaagacaa tcaattttgt
    35581 ctatatcgtc tatatattct tctattttca attcaatttc atcatctaat ttatatccat
    35641 tcttattaaa gaatttgatt ccattatatt caacaggatt gtgagatgca gatataacca
    35701 caccacaatc agcaccatat tttctagtta aatatgccac tgcaggtgtt ggtattattc
    35761 caacagtaat aacatcacat ccaacagaca taagtcctgc tattaaagat gcttccaaca
    35821 tatcacctga tattctagta tccttaccta ctactacttt tactctgtgg tctccttgag
    35881 ctaagacaaa tccaccagct ctaccaagtt tatatgctaa atcacatgtt agttctgtat
    35941 ttgctactcc tctaactcca tctgttccaa aatactttct cactaaagat tctcctttca
    36001 taattgaaaa gcttgtaatt tgtattatat ttttatagtt cataaaatat cattataggc
    36061 ttaatttcag tcataaaaat attttaatac ttatcagcaa aaaagacaat agaaataatt
    36121 ctattgtctt ttttttgaca tctttttctt tattagttag ttgctttaga acatgtagca
    36181 tccatacctt tttgtaaagc ttctctgttt aatggaacga atttttcttt tctaggtccg
    36241 aaaacttttt tgaatgcttc tagtacagat tctatatcta cagtatcaga tacttttaag
    36301 taagcgccta acattatcat gttagcaact ttatcatttc ctaattcagc tgctaattca
    36361 tttgctggta tataataaac atctacatct gttctatcaa ctttttcttt tattaaagaa
    36421 ctatttacta ttatttttcc accaggaata acatcatctt tgaatttttc aaatgctggt
    36481 atatttaata atatagctgc agttgcatca tctgttataa gtggagaacc aactggctca
    36541 ttagatacag ttactgcaca gtttgctgta cctccacgca tttctggtcc ataagatggt
    36601 aaccaagaaa cttcttttcc ttctagcatt cctgcataag taagtaattg tcccatagac
    36661 ataacacctt ggcctccgaa gcctgcacat attactctag ctgtagacat ttacttcacc
    36721 tcctcaactt caacattttt gaaatttcct aatggatagt atggtatcat gttgtctctt
    36781 aaccattgta aagcatcgtt tggagctagt ccccagtttg taggacaagt agataatact
    36841 tctactattc caaatcctaa tcctgcttgt tgaacttgga atgctttctt tatagctttt
    36901 ttagcttgtc taacatgagc tggagtatca actgctactc tttctacaaa tacagctcct
    36961 gttaatgtag ctaacatttc acttactctt attggataac cttgagtttc agcatttctt
    37021 cctgattgag cagtagtagc tctttgtcca actaatgtag ttggagccat ttgtccacca
    37081 gtcataccat aagttgtatt gtttacaaat atagtagtta ttttttctcc tctagcagct
    37141 gcatgaacta tttcagcagt accaattgaa gctaagtctc catcaccttg ataagtgaat
    37201 actgtatttt caggatgaac tctttttatt cctgtagcag cagctggcgc tctaccatga
    37261 gcagcttctt gagtgtcaca gttaaaatat ttatatccta aaactgaaca tccaactgga
    37321 acaacaccta cagcatcacc taatactcct agttcttcta atacttcacc aactaatcta
    37381 tggattatac catgtgtaca tcctggacag tagtgagttt gagtatcttg taatccttca
    37441 gttttcttaa atacaacagc cattatctag cacctcctgc aaccaattct ccggcaataa
    37501 tttttttagc tttttcaact atttctgctg gtgttggagt cattcctcct ggtcttccat
    37561 ggaagtaaac tggtaattta ccttctactg ccattttaac atcttccacc atttgtccca
    37621 tactcatttc aacagtcaat atgtttctag cattaggaat ttggttgaat gcttcgaatg
    37681 ggaatggcca taatacttta ggtcttataa gacctgcttt tattccttct tctcttaata
    37741 tatcaattgc gctttttact actcttgaag tagttccata agctgcaaat acaaattcag
    37801 catcttctgt tttgtacatt tcgtattgta cttcattctt ttccattgca tcaaattttt
    37861 cttgtaaatg ccaacaatgg tcttctagta cttctggctc taagtataga gagtttatta
    37921 cattaggttt tctttttcct ttagttccaa cagttgccca atctttagga ggcaattctc
    37981 ttttcttctc aggagctcta aattcaactg gctccatcat ttgacctatc ataccatctg
    38041 caactaccat aactggtgat ctgtaataat ctgcaacgtc aaaagcttcc attatcatat
    38101 caacagcttc ttgaactgta gctggtgcga atactggagt tctgtagtct ccatttcctc
    38161 cacctcttgt agacataaag tagtcagctt gtgaaggttg tatacttcca agtcctggac
    38221 cacctctcat tacattaagt acaacacaag gaacttctgc tcctactgca tatgttatac
    38281 cttcttgctt taaagcaact cctggtgaag atgaagaagt cataactctt gctcctgcac
    38341 ctgctccacc ataaaccatg tttatcgcag aaacttctga ttcagcttga acgaacgctc
    38401 cacctatttt aggtaactct cttgataaat attctggtaa ttcactttgt ggagttattg
    38461 ggtaaccgaa gaaatatttg caaccagctt ctatagctgc ttttccaaat gcttcgttac
    38521 ctttcataag tatcttagcc attaaaattc ccccttaaat attaattaaa ttaatctctt
    38581 tctactgtta taactacatc tggacacata gttgcacaac ttgcacatcc tatgcatttt
    38641 tccatttcaa aaacagttgc tgggttatac ccttttttgt ttattacatt tgagtctaat
    38701 tgtatgattt ttactggaca tgcttcaaca cacaatccac aacctttaca acgttcttga
    38761 ttaaaagata cttttccttt agccatttac ctaagcctcc ttaaactttc tatatatttg
    38821 agctcaaact acatccagtc ttctctcata tataatttta taggaaaaat ttctccttct
    38881 aaatcttttg gtaactgttc aacaagattt tctaaacatg caacatatct tatcggtata
    38941 ttacattttt cagaaacttc ttttgcaact ttctgacctc ttaaaacatc atcaatagta
    39001 gtaaacctaa taagatgagt attattaatt agacctgtta cctttaattt agaagatttt
    39061 tctatttctt ttatatattg gataacttct tcagaagttt gagttttctc cctatttgca
    39121 tttattacaa aaagcatatc ataatcgcct tcttttaaca aatgactaaa tcttcctata
    39181 accctagcac ctacattatc tccacctaaa tctataacag aattgtatga atggtctacc
    39241 ataggagaca ttacctctgc tgatactgct ggtaaatcta atgttggagc attaattgag
    39301 ctatctattg gttgtatacc taaggatttc attaactctt tcttttctct agttctaaag
    39361 tatacattta ccacatctaa atcggctata gcaactttgc cagaaaccat atctcttaac
    39421 ttaacaacat agttcatcgc aaattcactt ttaccacttc catagtgacc agttatgatt
    39481 cttattcttt tatcattagt tatcataaac ttgagcctct tcttcaccag ttaaaactct
    39541 aagtccacct tgagctaatg caatcatttc atcttcacct ggataaactt ttacatctgc
    39601 tataaattta actctatctg caatcatttc tgtaaacatt tttgaatatg cgattccacc
    39661 agttaataat attgctttta catctccctt aagaactgca gcactagctc ctatttcttt
    39721 agagatttga tatgccatag cttcatatac taatttagct ttttcatcac cagcttcaat
    39781 tctttcttca acttctctag catcattagt gtttaagtat gcaactaatc cgccattacc
    39841 ttttattttc tttttaattt catcttgagt atattttcca ctaaagcaca tttttactaa
    39901 tgcacctact ggtagtccac cacttctttc tggagagaaa ggtccttctc catctaatgc
    39961 gtttgcaaca tctactattt taccattttt atgagctcca acagaaactc ctccacccat
    40021 atgtgcaact ataagattta tatcttcata tttcttgttt atttctctag catatcttct
    40081 tgctattgcc ttttgattta aagcatgtac tacacttgct ctacttattt caggcatacc
    40141 agaaattcta gcaacatctt ctaattcatc tacaacaaca gggtctacta tgtatgaagg
    40201 aacatttact tcttcaccta tttgttttgc tattattcca cctaggtttg aagcgtgttc
    40261 tcctaaaact cccactttta aatcttcaat catagcagca cttactgaat aagtaccacc
    40321 ttttataggt ttaagaagtc ctcctctacc tactacagca tctaattcag atgtttttac
    40381 tccaccttct tttagagctt cttctattac ttgtttacga aattcaaatt ggtcagacac
    40441 cttctcatat tttcctattt cttctgaaga atgtcttaaa gttttttcaa atactaaatc
    40501 ctcattatca aatacagcta ttttagttga tgtcgaacca ggatttattg ttaagatttt
    40561 aaatattttg ctcatttgcc tatttccccc ttaaaattat gcctttgctg ccattaaaac
    40621 acctaaagct attgagttta gtttagtttc ttcactgtct gctctagaag ttaatattat
    40681 tggtgcttta gccccaacta taactcctgc attttttgat tttgagaaga ataccaaagc
    40741 tttatataat atgttaccac cttcaatatc tggggctaat aatatatcag ctcttcctgc
    40801 tacaggatga tttatacctt tatgtttagc tgcttctaaa gatactgcat tatcaattgc
    40861 aaaaggtcca ccaaccatac aacctttgat ttctcctctt tcatacattt cttctagttc
    40921 tttagcttca actgtatctt tcatttttgg atttactttt tcttttgcgc atattgcagc
    40981 aacttttggt tcacttatat ctaatgaatg tgctactgtg caagcatttt ctatgatttg
    41041 ctttttagta tttgtatcag gagctaagtt catagctgcg tcagttacga aaaataatct
    41101 atcatatccc tctacatcaa atactgctac gtgacttaat acatttccag ttctaagacc
    41161 tacttcttta tttaaaactg cttttagtat tattgatgtg tctactaagc ctttcattac
    41221 catgtcggct tttccttgtg aaactaattc aacagatttt agagatgctt ctgctaaatc
    41281 ttttatatct atcagttcat aattttcgat atccatgtct atgctttttg caatttcttt
    41341 agtcttttct atatctccta ctaaaatggc atttgctatt ttttcttttc tagccatttc
    41401 aactgccatt aaaacttctt tatcttggca acatgctact gatatagttt taggtcctct
    41461 ttcttttgca aacttaatta cttcttcaaa acttctcatg catttccacc tcatcttgaa
    41521 ttttttgaaa tagaactttt tatatctcaa ttaaattata actcgttcag caacgttttc
    41581 ctagagataa ttgaaatatt taattcaaaa tatttattta aatctattta tcagtctttt
    41641 ttcatatatc tagcgctttt tagatttaac aaatatctca ttgtccttat tttataatat
    41701 aactaaaata aacaagatgc aacaaaaaca taaaattaac aaacttaatt ttataggttt
    41761 ttttgtcgca tcttgtaaca actaaatatt cgaaatattt gttcattcca cttcaacaat
    41821 attaggttct atatttattt ttttaaattg atatgttgtt tcatatttta ttttatattt
    41881 actatcttcc tcaggacctt gtgataaatc aatatataat gaaatatctt ttttatttaa
    41941 cttactcaaa tcctccgttg actctacaac aacttttata tctacatcag aaattacatt
    42001 tggattatcc atatttgttg ggatatttct taattcaata tctcccgcat tatataaaaa
    42061 ttctgcactc aattgttttg tatcatttaa tttaattgtt atagagctat cctcaagagt
    42121 tataccctct ggaatttcta agctaacttc tttttcttca ccattagcca aatcagctaa
    42181 gtcaataggt tttgtcttaa tataacttat tttgtctata tcttctttct ttccttttat
    42241 tgtaacttgt tccttactta atttatagtt tttcagtaca tcattatctt tattatcatt
    42301 tagacttact cttattggga caattttttc cttcaatagt tctatctttg cagtcacaaa
    42361 ggatgaactt aatgttactc cttcaacctt agttccatca ctatttaatg gtattaattg
    42421 caatttttta gaaaaatcat ctgtctcatt accaacatct agattagctc ttacactttt
    42481 gacttcttta actagagttc ttggacctga tacatcgaca aattccttac taagttcaat
    42541 tttatttata tttctttttg aatttccact tacttctacc tttatatctc ttttttcttt
    42601 tatgtttttt tcaagattta ctactaaaac atcatgctta aattctagag taacaccttg
    42661 aggaatattt gcttttaaat atacttcctt ctgtccttgt attgggtctt caatggttcc
    42721 atatacatgt atatcttctt ttttaacact ttgtaccttt gataaactac cttttatata
    42781 tacatctgct gttaattttt cgtctggata tataacaaga tttttatcct ttaaatcact
    42841 catattactt atagatacag gtatatcttc aaacccttta gtttcttcag ggtcaactac
    42901 agccataaca tacatccata atgcaattgc acttaaaaat gatattattt ttattttggt
    42961 attatttttt agtctattta tcatttaaat ataccacctt tgaaaaaact tcttgtttct
    43021 gtatttgttt ttaaattact acgtaatata ttcatcattc tttctctaga aatatctcta
    43081 tataacttac ctgctttagc aattgatacg ccacctgttt cctctgatac aatcaaagtt
    43141 atacagtcag acacctcact tacccctatt cctgctctat gtctagtacc caaatcttta
    43201 cttaaatcct tactttctgt caatggcaaa aaacatgcag ctgcttttac cttagaatct
    43261 ctaattacaa cagctccatc gtgtaatggt gtatttggta taaatatatt tattaatagt
    43321 tgcctagaaa tttcagcatc aataatagtt cctgtattta ttatatcact tattctagtt
    43381 tctctctcca ttatgataag agctcctatt ttttgtctag aaagtgaata taaagcttct
    43441 acaatttctt ctatatttct attgagagtt tcttcagaag tatttacatt ctttccaaat
    43501 aaattaaact ttgctcttcc tatatgttca agtcctgctc taaattctgg ttgaaaaatt
    43561 ataagagcag ctataactcc ataatctaaa gctttaagag atatccaata aacggtatgc
    43621 aatttaaagg tgttactaag ttgagttgct aataaaagaa atattatacc tttaaacact
    43681 tgttctgctc ttgtatcttt tataaacata aatattttgt aaaaaatata cgctactata
    43741 gatatgtcaa ttaaatcata tatactcatt cttaatatta tattaaaaaa tccatttaaa
    43801 aaagaattta tatctaacac agttttatcc tcctgtttga taacttctta attatttaca
    43861 ctaagtatta tactatataa atctaactct atacaaacta tttaactttt atgtaaataa
    43921 ttctagtttt atatttggag attgttaaat aatctctaaa cacattaaaa atatcaactt
    43981 aataaataca tatttcttat taaagttagt ttttactcaa ataactcaaa ccatcaaggc
    44041 gcgaatacaa aatagtactc gcgccttaat gcagttttgt gttctaaaat atcttaatat
    44101 atctttgttt aatatatatt aattattata aattctataa tccacttatt aactataaac
    44161 aaatacaaaa tctaaatatt acttatcatc aatattattc cattagattc atttttatat
    44221 taaagtaata acttatttca tatctagatt agataaattt attttaatat cttactataa
    44281 actatcatta aaacttatta tgtataacta gatataatct cctacagtga aaataataca
    44341 caatacctaa agttatatat ccatttcaca g
//
LOCUS       DAEMHZ010000014        44207 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000014 DAEMHZ010000000
VERSION     DAEMHZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.014"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(289..1161)
                     /locus_tag="KPK72_000695"
     CDS             complement(289..1161)
                     /locus_tag="KPK72_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2487939.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1555..2133)
                     /locus_tag="KPK72_000696"
     CDS             complement(1555..2133)
                     /locus_tag="KPK72_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2487940.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2191..2907)
                     /locus_tag="KPK72_000697"
     CDS             complement(2191..2907)
                     /locus_tag="KPK72_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2487941.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3098..4183
                     /locus_tag="KPK72_000698"
     CDS             3098..4183
                     /locus_tag="KPK72_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2487942.1"
                     /translation="MIIISRENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4338..4997)
                     /locus_tag="KPK72_000699"
     CDS             complement(4338..4997)
                     /locus_tag="KPK72_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2487943.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5214..6506)
                     /gene="lysA"
                     /locus_tag="KPK72_000700"
     CDS             complement(5214..6506)
                     /gene="lysA"
                     /locus_tag="KPK72_000700"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG2487944.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6524..7717)
                     /locus_tag="KPK72_000701"
     CDS             complement(6524..7717)
                     /locus_tag="KPK72_000701"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2487945.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8078..8695)
                     /locus_tag="KPK72_000702"
     CDS             complement(8078..8695)
                     /locus_tag="KPK72_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487946.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(8865..10565)
                     /locus_tag="KPK72_000703"
     CDS             complement(8865..10565)
                     /locus_tag="KPK72_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487947.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(10962..11525)
                     /locus_tag="KPK72_000704"
     CDS             complement(10962..11525)
                     /locus_tag="KPK72_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487948.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11684..12397)
                     /locus_tag="KPK72_000705"
     CDS             complement(11684..12397)
                     /locus_tag="KPK72_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG2487949.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12689..14353
                     /locus_tag="KPK72_000706"
     CDS             12689..14353
                     /locus_tag="KPK72_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG2487950.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14460..15359)
                     /locus_tag="KPK72_000707"
     CDS             complement(14460..15359)
                     /locus_tag="KPK72_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG2487951.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15386..16024)
                     /locus_tag="KPK72_000708"
     CDS             complement(15386..16024)
                     /locus_tag="KPK72_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2487952.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16361..17359)
                     /locus_tag="KPK72_000709"
     CDS             complement(16361..17359)
                     /locus_tag="KPK72_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG2487953.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17457..18032)
                     /locus_tag="KPK72_000710"
     CDS             complement(17457..18032)
                     /locus_tag="KPK72_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487954.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKNLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            18348..18611
                     /locus_tag="KPK72_000711"
     CDS             18348..18611
                     /locus_tag="KPK72_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487955.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(19048..19425)
                     /locus_tag="KPK72_000712"
     CDS             complement(19048..19425)
                     /locus_tag="KPK72_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2487956.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            19768..20880
                     /locus_tag="KPK72_000713"
     CDS             19768..20880
                     /locus_tag="KPK72_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2487957.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(21088..21564)
                     /locus_tag="KPK72_000714"
     CDS             complement(21088..21564)
                     /locus_tag="KPK72_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487958.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(21763..23388)
                     /locus_tag="KPK72_000715"
     CDS             complement(21763..23388)
                     /locus_tag="KPK72_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2487959.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(23726..24745)
                     /locus_tag="KPK72_000716"
     CDS             complement(23726..24745)
                     /locus_tag="KPK72_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG2487960.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(24742..25311)
                     /gene="mocA"
                     /locus_tag="KPK72_000717"
     CDS             complement(24742..25311)
                     /gene="mocA"
                     /locus_tag="KPK72_000717"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG2487961.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            complement(25418..26140)
                     /gene="yqeC"
                     /locus_tag="KPK72_000718"
     CDS             complement(25418..26140)
                     /gene="yqeC"
                     /locus_tag="KPK72_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG2487962.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            complement(26323..27600)
                     /locus_tag="KPK72_000719"
     CDS             complement(26323..27600)
                     /locus_tag="KPK72_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2487963.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(27739..30045)
                     /locus_tag="KPK72_000720"
     CDS             complement(27739..30045)
                     /locus_tag="KPK72_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2487964.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(30038..30832)
                     /locus_tag="KPK72_000721"
     CDS             complement(30038..30832)
                     /locus_tag="KPK72_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2487965.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGI
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(30939..32297)
                     /locus_tag="KPK72_000722"
     CDS             complement(30939..32297)
                     /locus_tag="KPK72_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2487966.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(33644..34735)
                     /locus_tag="KPK72_000723"
     CDS             complement(33644..34735)
                     /locus_tag="KPK72_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487967.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMIVMKEDMSEVRQEINIMKEDISEVRQ
                     EMTVMKEDMSEVKQEINVLKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDVSEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(34965..36050)
                     /locus_tag="KPK72_000724"
     CDS             complement(34965..36050)
                     /locus_tag="KPK72_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2487968.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(36086..37399)
                     /locus_tag="KPK72_000725"
     CDS             complement(36086..37399)
                     /locus_tag="KPK72_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2487969.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNIEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLGILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(37400..39691)
                     /locus_tag="KPK72_000726"
     CDS             complement(37400..39691)
                     /locus_tag="KPK72_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2487970.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKGSDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(39681..40475)
                     /locus_tag="KPK72_000727"
     CDS             complement(39681..40475)
                     /locus_tag="KPK72_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2487971.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(40514..40960)
                     /locus_tag="KPK72_000728"
     CDS             complement(40514..40960)
                     /locus_tag="KPK72_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2487972.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(41032..42438)
                     /locus_tag="KPK72_000729"
     CDS             complement(41032..42438)
                     /locus_tag="KPK72_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2487973.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(42456..43826)
                     /gene="hydA"
                     /locus_tag="KPK72_000730"
     CDS             complement(42456..43826)
                     /gene="hydA"
                     /locus_tag="KPK72_000730"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2487974.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(43959..>44207)
                     /locus_tag="KPK72_000731"
     CDS             complement(43959..>44207)
                     /locus_tag="KPK72_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2487975.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIG
                     FGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSVYGK"
BASE COUNT        14078 a         7860 c         4609 g        17660 t
ORIGIN      
        1 ttaataaaat attatatact taaatacaaa taaaattaat tttataattg ttaattaagg
       61 aaatgatatt gtttagagaa ataaaaaaag cactctccat gaatggaaat gctttcttta
      121 ataaattagt tgacgtagta aagatgtatt cagaatacat ctttatgttt tgagtctcga
      181 aatatttatt tgtattattt aaacgaaaat ttatttttta agtgataatc gagtatttac
      241 taataaggct atgattctca atttcatagc cttattaata tacaaacttt actcatacac
      301 agaatatctt ttccaaattt gctctaaatc atcaaaatgc tgtttaatat cttctttttc
      361 cttcatccca acagatatag caagggcatt tgaaatacta agagctggaa ctagagaatc
      421 tacaaatgaa gccatattac tcttaactag aagagtgtta tctgcaagtg atgctactgg
      481 cgcaaataag ctatctgtta atgatattac atgggctcct ttttccttag cataattgac
      541 aatttgataa gatttcttag aataacgagg gaaactaata gcaactatta catcttcttc
      601 atttattctt acaatctgct caaaagcatc tcccatatcc attctaatta tatgaacatt
      661 gtcaagtatt atatctaagt aaaaacctaa atattgagct actacaaaag aactcctcat
      721 tccaagtata tatatttttc tcgctcttaa aagtttgtta gaagcttctt taaacgctct
      781 ctcgtcaatt tcttctagtg tagaccttat attatctata tcacttttta aaactttgtt
      841 tagtattgca aaatcatcgg aataatcatg tgccatttca actctttgta ctgtagttag
      901 tttatttttt atgagttctt gaagtgcagc ttgtaattta ggatatccac tataacctaa
      961 tgcatttgca aatcttacaa ctgtagactc acttactcct acagtttcac ccaatttaca
     1021 tgcagtcata aatgcaactt tatcataatt atttaaaatg tattgtgcta taagtttttg
     1081 acccttactc aatctcgtat attgagattg tatatttgat atcaaatgtt tactgtcctt
     1141 catttcattt gtttcttcca tttttttctc cttttagtta tataaactat tttgttttat
     1201 taacactgtc tttttctcta aaaattttat tttttatctc aatttcatct tagtttttat
     1261 catagcttct taaactcata aaattctaac ttttctgtac cttatacttc acatagtatt
     1321 ttcgattaaa tttattatac catctttcct tgcatttttt aatatttatc caatatttct
     1381 tttaaatttt tcaattttta ctcatttaaa agttatctat cttattttgc atatttagat
     1441 gtatattttg ttttgagaat aagctgttgt atacttatta tttaaagtat ttttaatagg
     1501 tcacactata tatattaact cttaatacaa tcttgtcaag ttttttagta tttattatgc
     1561 ttttttcttt tcaattaaaa tcaattgtgg tgggttattg acttgattta aaaacttaga
     1621 ttctaaaaca ttaaattcat cttgatttaa attattgaca aggttatata tgcaatcttt
     1681 ttcttcttga cctccatcat gccctgtata tatggcaatg cttattactc cattttcttt
     1741 tagcatttct aaacttaact ttatagcctt taaagttgta tctggtttgg ttataattga
     1801 atgtttcgct cttggaagat aacccaaatt aaataaaaca catgaaactt cttcttttat
     1861 atattgattc attttttcat gcccatcaaa tatcaatttt actctatctg tatatccttc
     1921 tttctctagt ttctttcttg tagactttat tgcttcttct tgtatatcaa aagcatatac
     1981 aaagcccttt tctccaacct tctcagctag ataccttgta tcatacccat ttcccatagt
     2041 agcatcaatt actatgtcac cttcatttat aatattttct aaataaaatt tatttatatc
     2101 tgtaatttta gttaaatact ttgcatatgc cataatacta tttctcccta ctattaactt
     2161 tatttcattt tttctcaatt tatttttatc ttattttatt gattttaaat attttatttc
     2221 atcttctgtt aggtatctat attctccaac tttggtgtct tttaatacta ttttccccac
     2281 agaaatcctt cttaaattta aaactggatg atttatagct ttgcacattt ttcttacttg
     2341 tctatttcta ccctcatgta tagttatttc acacactgaa taattttttt ctttattttc
     2401 tttagttacc cttattttgg caggtgccgt tgtgtaatct tctattttta gtcctgtttc
     2461 gaattgtctt atctcatcat cacttataat tcccttcaca cttgccacat acgttttatc
     2521 aatttcatgc tttggatggg taagcttata tgttaaatcg ccatcattag taagtattaa
     2581 taatccacta gtttcataat ctaatctccc tattggatat actctttccc ttacatcttt
     2641 aagtatgtct attacactgg gtctattgaa ttggtctttt actgtagtta tatatccctc
     2701 tggtttattt aatactatat atatataatt ttcacttggt tttacttttt tattatcaaa
     2761 ttctactatg tctttttctt catcaatatt aaaagataac tcttttacta cacttccatt
     2821 tacctttatt cttcctccaa gaattatttc ttctgctttt cttcttgatg ctattccaca
     2881 agaagctata tacttattaa ttctcatact taatccttcc ttatcttaaa ttatgtgtgt
     2941 ttaatatttt tatcaatgtt aattttgcta cattattata ctatactaga ccttaattaa
     3001 aaacaagcaa aaaaattatt taattttcct aaataattgt aatttctttt cacatactat
     3061 taatatgtaa tagctgacta aacactattt taaggaagtg attattattt caagggaaaa
     3121 tataaaatac tatttaatag tcgtgtttat tggaatttta ttttttaaat ttattaatac
     3181 tccatcagac tttatatcta gtattgaagg attcttaaag ttttttagcc catttttatt
     3241 agctatttta cttgctttac ttttaaatcc acttgtgatg ctttttgagg taaaattcaa
     3301 agcacataga cttttagcca tttttttatc ttatatattt ataggtttta ttttagcctt
     3361 tgccattagg ttattaattc catcaatagc taatacatta aatagattga taaatgaaat
     3421 gcctatgtat actgattata tagatagttt tatagaaaaa aatatgtcaa acatagattt
     3481 tctaaaaact ttaattcctc atatacaaca tagtttagat aatttattaa aagaagcaag
     3541 taattttgtt ggtaaaattc ctaaaaactt cttaatctat acacttagta tatcttctat
     3601 gctatttaat atgacaatgg gatttatact atctatatac attatatatg acaaagaaaa
     3661 aattgcattg ggatttaaga gatttctata ttcttctact gctagaaaca aggcagataa
     3721 cattatagaa ttttttagga ctactcatga tattttctat gattatttac ttggtagaat
     3781 attagactct cttattattg gtataattgc atttttagga tttcaatttg ttatcagaat
     3841 agaaaatgct ttatttttag cttcaataat attcttaggt aatataattc cctattttgg
     3901 tccttttatt ggtgcaatcc ctccaatagc catgactata ttatatagtc cacaaaaaac
     3961 tatatgggtt attgcatttc tatttatatt gcaacaatta gatggaaatt ttatagaacc
     4021 aaaagttatg ggtaatcaag ttggtatagg agctatatgg gtaatatctg ctattttggt
     4081 gggacaatct ctatttggtt tcataggagt ttttctttca gtaccaatag ctgctgttgt
     4141 aaaaacttat gttgataaat atatagataa tcgtttacaa tagcttatct ctactgtgac
     4201 tttaattttt agtttaaaat ttaactgcta tttattaaat tatttctcaa aaaatatctc
     4261 ttagtaagaa aaaattgcta agagatattt tttataatat ataatttgtt cattaaattt
     4321 atgcatatat tttatactta ttgattattt gagttttgag aatttccacc actatctgaa
     4381 ccaccagtag tatcttgtgg agttggtgct ggtgttggat tactattatt tggcttttct
     4441 tgattttgag aagaactgtt attattgtca gacttattac tatctgattt tttatcatta
     4501 tttttagaac tagatttatt atttttacta cttccactat aagaagattt ccttgcatct
     4561 gattcaactc tatacctatc ttctccagcc gtatcttttg cctttttctt tctttgttcc
     4621 tctgcttcgt tttgttcttt ttctaattta gttaactctt ctttcttttt agttgcctct
     4681 tctttaataa cagaggaacc atttttgatt ttctctataa gtttaagttc cttgattgca
     4741 ccctcataat tttttaattc ttcaaactcc gtcatatttg acattctcat agcttgcata
     4801 tacatagctc ttgcattctc attagtacta tcttcttcca gtgcagagga taatgattcc
     4861 atagcttttt tatagttatg tttttctaaa tatgtttttc cttgctctac taaatttatt
     4921 tcatcttttt tattaaatga acatcctaca agaaatatag ataatatcga tattaataaa
     4981 attactcttc ctctcattaa atccctcctt gtcatatact ttaaatttta tcataattat
     5041 aaagttttta aaatatttta tatttattac aagaaatttg ttagtaaaac aaaaaataag
     5101 cagatatgct acgaacgata caataaacca aaaattaatt tagagataca tatcctagca
     5161 aatctgctca taataaactt taatatttaa tttttattat atacttctcc attttatatc
     5221 tctaaagcta ataagtcctc atagctttgt ctcttacaaa ttatcttatc atttccatca
     5281 aatactgata ctactgctgc tcttggtatt ttattataat ttgaagacat tgaatatcca
     5341 tatgcaccag ttgatgttgt tataagtata tctccacttt taatatccat tatgtttata
     5401 tctccaatta atatatctcc actctcacaa cattttccag ctattgttac atgattcata
     5461 gcctcatgat taattttatt cacaatacta cactcataac tagcttggta caacgaaggt
     5521 cttacattat ctgtcatacc tccatctaca cttacataaa ccctaacatc ttttatatct
     5581 tttatagaac ctactgtata caatgtactt cctgcatttg caactattga ccttcctggt
     5641 tctattacca atgtaggtac ttctattcca agctctttac atgaagtttc tgctttagtg
     5701 attatggttt cacaaaactc ttttatagtt ttaggtttat ctccttcgct ataataaacg
     5761 ccaactcctc cacctaggtc aatttctgtt agttgtatgt tgaatttttc ttttatttct
     5821 tttaccaagt tcatcattat atccactgta tctatgtatg gttctacatc aaatatttgt
     5881 gaacctatat gagcgtgaag acccacaagt ttaatatttt tatattcact taatttttct
     5941 attgctctaa ataaatctcc atttactaaa gcaaatccaa atttagaatc tatctgtcca
     6001 gtttttatat aatcatgtgt atgagcttct ataccaggag ttattctaaa gtatatctct
     6061 tgagttttac ctttatcttc acataatgct tcaagcaaat ctaattcata aaaattatcc
     6121 accacaaatt tacctacacc taaatctaat cccatgttta tttcttcttt agtcttattg
     6181 tttccatgaa agaaaacttt ttccatagga aaatttgatt tataagctgt atataactcg
     6241 cctcctgaaa ctacatctaa acacatttgt tcttcattta taagattaca catgtaaaga
     6301 ggtaaaaaag ctttccctgc ataagcaacc ttattttttc cctctctagc cttaaaatat
     6361 tctatatatt ctctacaatt ttgtctaact aaagcttcat caaatacata aagtggagtt
     6421 tgatattttt tactaagttc taaacaactc actcctccaa tatataattc atcattattt
     6481 acagtcatag ttccatgaag tttcataata gcctccccaa attttatata ttaaatactt
     6541 ctgctatttt ttctatagct acttgcttat catcagaatt tatagcacaa gttatacgaa
     6601 tctctgatgt agttataagt tttatcatta taccattttc attgaatatc ttaaatactt
     6661 tagcagcaac tccagaagta tttttcatac caagacctac taaagaaaat ttagtgatat
     6721 tattatcaat cactacatgt tcttcatctg tatatttaga tactatcttt ttacattctc
     6781 ccaactcttc ttttggaact gtaaatgata cacttatctt atctaaaata ggtgcagttt
     6841 ggcttatcat atctacactt atacctatag ttgctatatc ttcaaataag ctagatatat
     6901 tttcagcttt aaaatctttt attgttatag aactgtcatc atcactagta gctaatcctg
     6961 ttattacttt atcctctaat ttcatgtctt ttccccctct tatatatgtt cctttttctg
     7021 taccacatgt acgtccgaca tagatttcaa caccatattt ttgtgcaagc tctatacttc
     7081 ttgaatgcat tacttgtgct cctaaactcg ctaactcaag catttcttca tattcaatct
     7141 catcaagttt actagccttt gaatattttc ttgggtctgt aaaataaatt ccatcaacat
     7201 ctgtatatat ctcacatttt ccatttaatt taaccgcaag agctacagca gatgtatctg
     7261 aaccacctct tccaagagtg gttacatcac catcttcgtt gattccctga aatccagtta
     7321 ctatcacgac atttccttca tctaaactat ttttaattct gtttacgttt atatcatcaa
     7381 tttgagactt tccatgaaga ccactcgttt ttatatttaa ctgataagca ttataactta
     7441 tagctttaca tccaagtgca tttaatgcta ttgataataa agatgctgaa atcatttcac
     7501 cagtagacat taaagcatct aattccctct tactaggttc attactcaat tcctttgcta
     7561 aagttatata ttcatcagtg gattttccca tggcagacac aactattacc atttgtggat
     7621 tttcttttct tcgttctatt atattttctg ctattgattt tattttttca gtatctgcta
     7681 ctgaacttcc accatatttt tgaacaatta tactcatgct ttctccccct caagctaaat
     7741 tttgataaat aaaaacgccc aaaggtatac tttggacgtg taaatttcat agaccaagta
     7801 accttgtagc tcaccacttt aaaagtgaca gccttatgca tattctgcat aagtccagaa
     7861 aatttgtccg tcaacaaaat ttcttcggct atataccctt tcatcaatta tcaacgctta
     7921 ccagctccta ttgaatacta ttgaatacag ctacctctac cctaggctca ttatttttaa
     7981 ttaatttatt aatatagttt tatcatattc tatataatat tgcaacaatt attttacaaa
     8041 atgtggcaaa tcgatagttt tcttttctaa agttagctta tatacaatta aatagtttat
     8101 ttaatcctaa atctataaaa tttatgctat actcattatc atatctctct aaatcattta
     8161 attcggatag attatctggt gtaactacca aaacgtttgg tactttaaaa aatggaattg
     8221 tagatttatc tattttttta gaaagatttt cgtatttaga aatgtctatt tcattattta
     8281 agtcaacatc aactaaaact tgctttatat taccaaattg gtctgtatat tcaataaata
     8341 aatcgtattt cacatcatct acactaacat ttcggtaaaa tctttttaca tcacaacctg
     8401 cagtattgat tctaacaact aattctgatc ctactagagc ctttattaaa tcatctccct
     8461 taaaaagttt actaccgttt aaatagtata cataagagag gtctttataa ttataataat
     8521 ccacatcaca aggatatttt tttagtgtat attcactaca catttttctc aaagtttttt
     8581 taaaattttt tttattgtat cttttttggt ctccataaat ttttctaaat tgagactctg
     8641 taacacatct aaccctgcta attaaaagta atatctcttt ttccatattc gtcatagtac
     8701 attctccttg aagtattatt tgcaaagttg tactaatatt atattaattt tatgacatta
     8761 aatttacaaa gaattaaaat aaaatagcta atttttatag aattaaattc tataaaaatt
     8821 agctatcact atgttaaata ctattatcaa tctttttatt taaattatac attaccttga
     8881 ttttcagaat ctttggaatc tgtattttca tcagttcctg ttttttcttt tgctttttta
     8941 tcttcaaagt ttgcaatatt taggttatca aattctgaca tttctattac accagattct
     9001 tcttttgaat acgatatttt gactttatca ccaacctgag tcaatggcaa ttcgctagat
     9061 acttttgaag tagccctaaa tattatatct ttatttgaat ctaaagttac ataataatta
     9121 gtgttaccac ctcgcacatc tgggcttatt cttgtcactg tgccttctaa tacctcatct
     9181 tttaaatcat tatcaagttt taaatcatta cccttagatt ctaatgcatc cttatagttt
     9241 cttaatgctt cattcttatc ttcacctatt ccaagtacat tataatcttc aacagataca
     9301 aatgcaactc tcttaacaag accagcctta tcttttaatg atgatacata tgttggctga
     9361 cccaatatat tatacattac agggaacgtc gcttcataat ttttttcttg aacattacct
     9421 tcagctgatt tcattgcagc cgtttcagta gctcctggtt gtttatataa tctagcttct
     9481 tttgttctag aatcaactaa cataaatcca attgtagatt catctccacc tgaagatgtt
     9541 atacctgtat accaataaga cctgttatca ttgccataaa ctaatgatgt accttctgtt
     9601 ggtactaaaa ctcccttttc tgatataaca gagtttaaaa atccattaac ataaacaccc
     9661 cagtctttaa tctggtctgt tacaaagctt tgtggttgta ttctatccac ccatttaggc
     9721 gtgtttttaa catcataaac atttattttt ccagtttctg catcaacagt ggctattcct
     9781 gttgcatttg ctcctccata gccaatttta tgttcatata aacttacaac ccaataaggt
     9841 cttccttcat catttatttc aagagtgaaa tctgtcatac caacatttac aataccatgt
     9901 atgtataaat gtctttgtaa atcctgatgt aagtaggctt ctggttggta aactatcttt
     9961 attggctttc catctatttc ttgaactaat tggacgtctt gaggattact agctgatact
    10021 tttacataac cacttgtacc acttagagat gttatccatt taattatgtc tctgtgaaca
    10081 agaggcgcta cccagtacaa ctctccatct acattttgta tatgaaattt tcctaatttg
    10141 gatacactcc ctattgcagg aacttctcca agctttttgt cacctaattt cattgccata
    10201 tcttcatcta caagacgaat gtcatttaca ctgacagggc taatgtcatc agtaaattta
    10261 ctttcagtta cttttcccaa tagttctcta tatgattttg catgaagtac aggtgtagaa
    10321 gttataaagg gaactactac aatatacaca agcaaagcta ttgctattga aaaattatat
    10381 ttagccattt tagatgctct ttcatttcta tctaacatca tatctagtat tcctgctaca
    10441 acaaagaaaa ttattcctac tgaaaataaa gatgtaaaat ctaatcttag tacaggtaat
    10501 ttaataaatg cataaattat tacaattatt aatgcaactc catatgtttt aagatatcgt
    10561 ttcatttaat actcctctct aaaaaatgtt attattacta ttatatccat atatttacaa
    10621 aaaataaata tttatcatat ttattatata ttaagatatc tttatatatt tatagttctg
    10681 cttattcaac tatatttaac attatacgtg tatgtataaa tactttttat acactgtttg
    10741 tactatccat cccgcaccta aaggagtggg atttttgcac tattctgtat tatatatatc
    10801 caaaaatatt ataaaaatac aaatattagc agaaatttaa aaactgaaga aagtattcta
    10861 actaagattt acaaattctt aatcctatcg aagttaaact actatcttca gcctaattat
    10921 ctagcaatat ttttttatat gaaaaactct aaatcaagtt tttaaatgtt tgcgattttt
    10981 gatacataag aatgttgtct agaattatat tctgataata aaccttcata ctctaaggtt
    11041 aagccagata aattataaaa atcatttggc gttacttcaa atgcttgttt atttaaaatg
    11101 tctatctcac ttttttctaa catgtcagca acaaaatttg aacatacata tttatattca
    11161 gattgtctct gaatattcat tagtaaatat attaatgcca ttgtatcgta atagtaatct
    11221 tctctataat cactaattaa ttttatattt ttatatagat tttcatattg taaattatct
    11281 acttctaacg aatataccct acacacagtg ttttttctta tagcatataa tccttgattt
    11341 atattttctt caacaaatcc tccaataaat ggattccttg gatttagcct tccaaaagaa
    11401 tacattggtt ttagattttc atttaatgat attgacacat gtgtatacag ctttttactt
    11461 atattcctta ttaagtaaga taatatagtt cctgtatatg ttgttacaat atagacttta
    11521 ttcatcattt ccccccaatc tagtaactaa ataatattat acacttacaa tttaatatca
    11581 taagatattc taacataata aatgtggttt tacaaatttt gattttaagg tactttaata
    11641 ttatatagta taataaaaat ataaatataa gttttctata tttttaacca acagatgttt
    11701 tcttcaaatt aatcagatga tttagtatat cttgaacctt tccagagtcc tttaaatatc
    11761 ctaatgcccc atttatttgt gattttaaat ctatttctac ttccatatcc aaatcaacat
    11821 ttcctttttt tatagatata tttttaagtg ttattggata aagataactt ttttccaaag
    11881 taatcttatt tccattaacc tcaaatggaa cttttttatt gtagttactt aatatttttt
    11941 ctagcatttt atcacttagt ttaaattttc caattttcac attctcaaga cttacaacta
    12001 agtttccatc ctttaaagta ggtctcaatt caagttcata ttgactattt accaaaccaa
    12061 ataccttata tggtcctgct acctttattt taccattttt catttcaaca aaattatttt
    12121 caagttcatt aattccatgc ttctgcatca atgtatatat taaattttta aactcttcct
    12181 ctgaaacaga tatttttcca accattctaa gtggattttt tactacctcc atagaattta
    12241 tatatgagcc ttttgataaa tattcttcat ctatgatttt attattttgt gaactaacat
    12301 catatctttc ttttggtgtc aagatatata caacaattcc tgcaagtact aaaacaataa
    12361 caattaaact taacaaaatc tttgcaacct ttcccataat cgtctccttt acaaatctgt
    12421 catcagattt aaaataaatt tagataacta acttcacata atattctcaa caatatacat
    12481 actataatac agtcttttaa aaattaattc aaccaatcta atgttttgta aataaactgt
    12541 aaaaacatgt ttttagtata aaagttaata ttttgcataa tactcttaat tacaccttta
    12601 tttttttcta atcttggatt aaaattaata ttatgcaata cagtgtagtg ttctgtatta
    12661 taatcaaatt aaataggagg tatagaatat gtcaaatgaa acgaactcat ctaactttat
    12721 aaagaatatt attataaacg acttagaaac aggaaaacat gacagtatca taactcgttt
    12781 tccacctgag ccaaacggat atttacatat tggtcatgca aaaagtatat gtcttaattt
    12841 tggattagct aaggagttta atggaaaagc taatttaaga tttgatgata caaacccatt
    12901 aaaagaggat gttgaatatg tagagtccat aaaagaagat gtaaagtggt taggatttga
    12961 ttggaacgag ttaaattttg catctaacta ctttgatgaa atgtataaga gagctttaat
    13021 actaataaaa aagggtaaag cttatgtatg tgatttgaca caagaggaaa tgagagaata
    13081 tcgtggtact ctaactgaac caggaaaaga aagtcctcat agaaacagaa ctattgaaga
    13141 aaaccttgat ttatttgaaa gaatgaaaaa tggtgaattt aaagatggtg aaaaaacatt
    13201 aagagccaaa atagatatgt cttctccaaa tataaacctt agagacccaa ttatatatag
    13261 aatatctcat tcaacacacc ataatacagg agataaatgg tgcatatatc ctatgtatgc
    13321 ttttgctcat ccaattgaag atgcaattga aggaataact cactctatat gtactttaga
    13381 gtttgaagac caaagacctc tttatgattg gtttgttaaa gaatgtgaga tggaaaatat
    13441 acctagacaa atagaatttg ctagacttaa tataaataat actgtcatga gtaaaaggaa
    13501 attaaaacaa cttgtagatg agggtattgt tgatggttgg gatgaccctc gtgttcctac
    13561 aatatccgga atcagacgta agggatatac tgcagaagca ttgcgcaatt tctgtagtga
    13621 aataggggta tcaaaagtta actctacggt agatagtcaa atgcttgatt atttcctaag
    13681 agaaaactta caaccaaagg ctcctcttac tatgggagtt ttaagacctt taaaacttat
    13741 tataactaat tacccagaag ataagattga gatgcttgaa atagagaata atgctaaaga
    13801 tgaatctcaa ggaaagagat tagttccttt ctctagagaa ttgtatatag aacaagatga
    13861 ttttatggaa gaacctgtta agaaatattt tagatttttc ccaggaaatg aagtccgttt
    13921 aaaaggtgct tattttgtaa aatgtactga tgtaattaag gatgaaaatg gaaatgtagt
    13981 tgaaatacat ggtacttatg accctgaaac taagagtggc tctggcttta caggacgtaa
    14041 ggtaaaatct acaatacatt gggttgatgc taaatcagct ataccatgtg aatttagatt
    14101 atttgaacca ttaattcttg atgatatacc tgaaaatgaa ggtaaacatt tcttagaaca
    14161 aataaatcct aattcattgg aaatattaca aggttttgtt gaacctacac agataaaaga
    14221 tgctaaacct tttgataaat tccaatttgt tagaaatgga ttctttagta tagataataa
    14281 atatacaact gacgaaaaat ttgtatttaa tagaatagtt cctctaaaaa gttcttttaa
    14341 accaggtaaa taatcttaca atcaaaaaat gaagacttaa aatataaatt acttctacta
    14401 taaataaact tagttagaag taatttttta tttgagtctt catcatttac tttaaatgat
    14461 taactttttt cactacagat tcccataact tctattccat cttttatgca ttctatgtta
    14521 agaggaaaat ctggtccttt ttctccgtca atatctgtta taagtgaatc ttcacattct
    14581 atatgcacct ttttagtttt aaagtatagt atttcatttc cattatattg atctaaataa
    14641 tcacctctaa gcacacttat caaaactggt attgattttg gtattggcat tcctttaaaa
    14701 ataattacat ccaaatatcc atcatctatt tcagccttat atgctagatt tatatttcct
    14761 gctgttttgc cattaaaaat caacataaga tacatatctc catcataata catttcctca
    14821 gaagatactt ttatcttaaa tttcctcata tacaatgctt cttctattcc ctttatataa
    14881 taagaaattc ttccaaatga attttttaag tctgtgttta tcctttgtga tacatctgta
    14941 aacataccag cacttgcaac atttataaag tacttattat tgactttccc tatgtctatt
    15001 ttctttggtt ttgaatttat gatttcattt atagcttctt ttatatcaaa tgaaatttga
    15061 agagcatttg aaaaatcatt tgcagttcct gtaggcaaaa ttccaattgg aatttttatg
    15121 tctaattctt tcattgcatt tactactaaa tctacagtac catctccacc agctatcaat
    15181 atgtgttcat aagaactatc aacttcctta aatgcattct ttacaggttc tttaatagtt
    15241 aatctatagg gtatcaataa ataatttttt tcttgatata attctatgat agtgtctaaa
    15301 tttgataata tctttttttc acctgaatta ggattgtata taagttttac ttttttcatt
    15361 atttctcctt actcatttta ttcttttagt atccaaaatc ttcatttaaa aaaatcacat
    15421 gtattctttc tggtgttaat tgtcttttta taagtgtata actacagcat attcttcctg
    15481 gacttgaaca gtctatacat ataccagtag tcttacatcc agtatttgta cttaatcttt
    15541 tacaattggc tggagcagct attgtttttg ttctttttat agcttcatct acatctttaa
    15601 ctattttatt tactcctgct ataactatta ctttatcagg tccatatatc atagcagcaa
    15661 ctctattccc attcccatct aaattgtaaa gttctccatc ctctgttatc gcattagtac
    15721 ttgtaaaata agcatctgca gaaaatgact ttctatatat ttctttcatt tcttctggtt
    15781 tgatatcttt tgcatatcta tcaagaaaat tatatttttc tcttataaaa tcaatcactc
    15841 ctgtttcaaa taaggtcata gaacctccac atcctactag agaacctact ttaactattt
    15901 cttcaatttt tttaagaagc tcttctttgt tacttactaa ataccctttc atattattgt
    15961 tttccagaga ttttattgtt ttttctattc ttttttcatt aagccaattt aaattttgat
    16021 ccattttaac ttccccctta tttttaatat gtacttaatt atttataaac tttattgtct
    16081 ttataagttt attttagtac atctaaaaaa aaatacaata taaaacttaa atagttttat
    16141 ataatttttt ccaaaataac ttactcaatc aaaaaaggaa agtagatgta ctattctact
    16201 ttccctatat tgtatgtagt atttattgcc taaaatctat atattaatta tatgtttcat
    16261 attatataat atcacagtta attaaaatgt aatactatct tctttttaaa catataaatt
    16321 gtattattaa atttaatata tccaacttca ataaatctta ttaatttgat gaagaagatg
    16381 ctatagctgc acttgttgct gccattacac atgcaatttg aatagcaaca acaatattta
    16441 aaaatatatt attagttata gagcctatat tattctcatt tcgtatcaaa tctgcataaa
    16501 ttgaagctac aatagcagaa ctaatcatta ctctattact ttttcctaaa gaccaatttc
    16561 caaagccttt ttcttcttta agtttatttg aaacattttt tatctcttct attatttcat
    16621 cttctctata atctaataat gaaattgctc ctattattgg atatccataa taattcattt
    16681 tacaatcatt ttctttaaat gcttttttaa gttcaatagt ctttttacat ttaagcatag
    16741 ttctatcatc actaaataaa agaatttggg ataatgcttg taaatcattt cctttataaa
    16801 aaccattttc atttagaaat tcataacatt tttctatgta ttctaaatct tcatctaaat
    16861 tttttgaatg aattgcaata agtgctatat tacaatagtc atcactagaa gttagaaatg
    16921 gatgtttttt cctcatgtaa tcatatatta ttttcatttt tgatatacat gtaagataat
    16981 ccattttctc tctattttca taaactattg atgctgtaag tggtaaatat gtatcgcccc
    17041 aaaacttttc acttttaagt tctttgtata tatctaaaat catcttaaaa ctactttctg
    17101 gatttgattc aaacgaaagt agtgttgcca tataaaacat attatatcct ctaaaattag
    17161 agaataatcc agtatttttt ttaataagct ctattcgttc ttccactaat ctaggactta
    17221 ttctcttgtt cttaagagta aatgcaagtg cacaactatg ttgtatcaat cccatttgcc
    17281 aagaaccttt aacttcattt aatgctttgt aattttctat taataagttt acttttccat
    17341 ttataaacga ttgttccata ttgccctcct agtaatatat ctcattgtac taatagtttt
    17401 atatttatta tatatatatt aaatactaca atcaatacat attacttcaa agaacatcat
    17461 tcttcaacaa taatgtagcc ttttttgtaa tgtcttccca tattattgac tctatcatag
    17521 tactctgatt cactttcatt actccatttg ggcacaaata catctattgc attagaatta
    17581 ctaaaatact tactagaccc cctatccata acagttttta attttccatc tacaataatc
    17641 ttagttccaa aactcaagtg attgtttgct acaaaaccat cttctaaatc atctcctgta
    17701 gcagttatcg ctccatatcc attttcttga ttaagacttg tgtaataact aacttcaaaa
    17761 gttataggta ctaaattctt tattttaata cctaactgat tttgtagttt ctccttttgt
    17821 actttcaact tatactcatc actattttta taatcttcca taccttgatt atacttggaa
    17881 ttaaagcttt tacctgactt tactatagta tctatattta ctatagattc tttacaatga
    17941 tctgcatcta tttttgtttc tccatttacc aataatggag ttaaacatac actacataca
    18001 acacatgtaa tcaatgcttc tttaagcttc ataaattccc cccttattat tttgatatat
    18061 tagcatattt aatattatat gtctacattt agatttaaat accacaccca tttgctttaa
    18121 gaagttgtct tatatttaat tcaccttaaa cacttataaa tcctctatct tagaatcata
    18181 tagaataaag cttttatctt taaatatttt ttattaatct tttaaaatct aaaactactt
    18241 tatctaattt tctctttttt agaatttaag aattaaaatt ctataatttt aaaacatatt
    18301 gaatttattt ttttgagatg atataatttt cttaagaggt ggttgatatg ataaaaaata
    18361 caaaacaaat tacagtagaa gttaatgaaa ttttattaca agaaatgtct aagataacta
    18421 atgacgaaaa taatattttg acagatttga taaatgaatc aattgtaaat tatattcttg
    18481 aaaacaaaaa acataatatt gaaaataaag atttagaaga agtgattaaa ttttacaaaa
    18541 aagaaaatac tgaaaaacct aaaagtgcag caagagctat agctgaagca aaaactcata
    18601 ctctgtatta atcaaataaa aaagttccta aaaactagga acttttttat attaataatt
    18661 attaatatat ctcaatattt atctcaaatc tatcaatata agcatgttta atacaatcta
    18721 ttgtatactt atcttaccct agaaataatc aaaacaaaaa taaaattatg ccctttatat
    18781 ctaaaacaat taatttacat gattaatata caaaaattct gacttttaaa ttcgtataca
    18841 atatcaaact aaaataatat taatgacaaa cccaatataa attaaattca aacaggtttg
    18901 attttacatc aaacttattg tattaatatt aaataacaag acaaaaatat ttataaaaat
    18961 cacaaatttt acataaaact ttatatttga ttcatttaac taattattgt aaaaactatt
    19021 tattttatga taattaacta caccaaatta ttcatctttt ttaatatcct taacccattt
    19081 ttcaacagag ttcaattctt cttcatttac cccctttttg aacaaggatt tcataaaact
    19141 ctcaatagaa cttccatgca tattatctaa aaaagttttt gtttcaaaat gtaaatactc
    19201 ttcttctcca ataagaactg tataatgtgt atgtctatca atttttcttg cacttaagaa
    19261 ttgcttattt actaatcttg ataaaagtgt tagtgttgta gtttgcttcc aattatatag
    19321 tttttccata gcttcaatga catcttttga tgctactgta aaatctgaac tccaaataaa
    19381 tttcatgact tttaattctg ctggtggtat cttagtaata gtcatatgat tttacactcc
    19441 tctcaaactt ctacataatt aaatactata ttaatagact tttcgagtca aaggttttat
    19501 ttaaaatatc tcatccaaat ttattaatac aaaaaattta tatttttatt caaaattaaa
    19561 cacacaatct attaatataa agataaatat ctattctata agtataacag attatcaaat
    19621 taaaattcta atatttgaca taatatatac attttttaaa taaatatata caatgtatta
    19681 aaatgtatgc aactctattt caaaattatt ttgtgatata tatatatgtt ataataaatg
    19741 tataaaattt tgattggtgg gttatatatg aatgcattta ttagaaaaag aaataaaaac
    19801 tatgttgttt acctggaatt tgaagatgaa aagacaggta aaagaaaaca aaaaaatatg
    19861 ggtacttttg ataaaaaaag ggaagcaagt aaaagactaa atgaggtaaa agaaagctta
    19921 tataatgatg gttttttagt accaagcgaa attacagtgt cagaatttct attagatttt
    19981 ctaaataaat atagtgaaaa tatatcttta gctacatata ataattatgt tagtatttgt
    20041 aagaattaca ttaatccatc tattggaaaa tataaaatac aagacttaca tccagttcac
    20101 atacaaaact acatagataa actatcttat aaactaaatc ctcaatcaat taagattcat
    20161 ataaatatat tgaaacttgc aataaaaaga gcttatagac tcaaattaat aaaagaaaat
    20221 gttatggata gtatagaggc tccaagatat aaaaaattta aaaatgagat ttatgataga
    20281 gaacaaatga taaaattatt agatttagca aaaaatacag aaatggaatt acctattaat
    20341 ttagctattg gattaggtct tagaatttca gaagttttag gtctaacttg ggataatatt
    20401 gattttgaag aaaatacaat aacagtaaat aagataactt cccgaattaa tggttctgtt
    20461 attctcaaag agccaaagac agaaagctct gttagaaaaa tatcttctcc caaagaatta
    20521 atgtctttat taaaagaata taaaataaaa cagaataaaa atttattaaa atcttccatt
    20581 agaaataata ataatttgtt gtttttcaat aagaaatgtg aacctattgc tgaagatgtt
    20641 atgagtaaaa aatttaaaag atttttagaa aagaatgaat tacctcatat tagattccat
    20701 gatttaagac actcacatgt tactttactt ataaattcta aagtacctat aaaggttata
    20761 tccgagagag taggacactc aaatataagt actaccctaa gcgtttattc tcatgtactt
    20821 aaagaaatgg ataaagaggc ttctgacaaa atttctgaaa gtttatttaa tgctaattaa
    20881 tattaattta tcttagcttt aaattagctt agcttattga gatagattca ttaatattaa
    20941 atgtatctat ctcaaattaa taactactta aataatctgt tttattttaa tttatcgcct
    21001 cagttattat acttttttac gaaaaattat actttacaat aaaaaagata ttcccttata
    21061 gaaaatatct ttttaaatac tatattatta tatacttgct aacattggaa tatctaacat
    21121 cttcttcatt acatcatcta ctttttttgc tttatattca ttgtatccaa ttaaatatcc
    21181 tttttcatat gaacttttta aaatctcttt ttcatataaa ttatcagatt tggcttctgt
    21241 gacacttaaa ttataaagcc ctttacaccc atcttcataa cctttattat accctttttc
    21301 tgcaaaatag tttttatgat ttttttcgtt atgtactgaa gatattgtta ctccagaatt
    21361 ttcttttgta ctagcataag gacaaaaacc tttattgtgt tgatgtgctg aatgtcctcc
    21421 acaataataa cgtgttaact ctgatttatt tttattatga tgacctcctg atgaattatg
    21481 ccctcctgag tgagcatatg aaaaattccc tacactaatc atagctaaac tcaatactac
    21541 agctactaac cttttcttaa ccataatcta ttccccccaa aatgttatat atttttatta
    21601 tatcatctac tatattatat ttaaattctt aatatacaaa atattactct attctgctaa
    21661 taaacatgtt taaacaacat aaaaaacctc ctaatatttg attaaaaatt tacacactca
    21721 tgcatagcat gtctcaaata ttaaaaggtt ttatactaat aattatttat cttttccata
    21781 cattttagct aattggtctt gtatctcttt actttctaaa tactcatcaa agtccatttt
    21841 tctatccatt attccatttg gagttatctc tataattcta tttgctatag tagatataaa
    21901 ttcatggtca tgagaagtaa ataaaagcac tcctgggaac ttttcaagtc ctttatttac
    21961 agaagttata ctttctaggt ctaagtggtt agtagggtca tctaatacaa gtacatttgc
    22021 atttgaaagc attagcttac taagcataca tctaaccttt tctcctccag atataacttg
    22081 agcttctttt aatgcttctt cacctgagaa taacattcta cctaaaaatc ctcttataaa
    22141 actttcactt ttttcttcag agaactgtct aagccagtca accaaagaat attctactcc
    22201 gtcaaaaaac ttattatgat tttttggaag ataatcttga gaagtagtta ctccccattt
    22261 atactctcca ctgtctggct caagttctcc cataactatg ttgaaaagtg cagtagtagc
    22321 tatttcatca cccataaata ctactttatc atctctatcc aatctaaatg acacattatc
    22381 aagaacctta accccatcta tagttttagt taaattgtgt acttctaaaa cttcattccc
    22441 aatttctctt gcaggtgtaa atcctacata tggatatctt cttcttgatg gttgtatatc
    22501 ttcaacttct aatttctcta actgtttctt tcttgatgtt gcttgtttag cctttgaagc
    22561 attagaacta aatctagcta taaattcctt taattgagct atcttttctt cagtcttttt
    22621 attttggtcc ttagccagtt gtaaagccaa ttgacttgac tcataccaga aatcatagtt
    22681 tccaacgtac atttgtattt taccaaaatc tacatcaact atattagtac atatttgatt
    22741 taggaaatgt ctatcatgtg atactattat aactatagat tcttctaaat ccattataaa
    22801 attatttaac cagtttattg atttaaaatc taagtggttt gtaggttcgt ccattaatag
    22861 tatttcaggc ttaccaaaaa gtgattgtgc taacaatacc ttaacctttt ccccaccaac
    22921 tagttccttc atttgtttgt aatgcatatc tttcgttatg ccaagaccca taagtatttt
    22981 ctcagcatta gtttctgcat cccatccgtc aagctctgca aactcacctt caagttcggc
    23041 agcttttatc ccatcttctt cattaaagtc ttccttcata tacaatgcat ctttttcttt
    23101 cattatgttc caaagtctct catgacctct tataacaaca tttaaaacag tttcttcttc
    23161 atattcaaag tggtcctgtt ttaaaactga cattctttct ttatcagtta tagatatact
    23221 acctgtatta ggctctattt ctcctgataa tatttttaaa aaagtagatt ttcccgcacc
    23281 attagctcct attattccat agcaattacc tttagtgaat tttaagttaa catctttaaa
    23341 tagttcctta tcaccaaatc taagtccaac acctgtaact tgtaacatta atatattttc
    23401 ctttcttttt gctcataatt tattttatca taaaactttg ataacatttt tattatatat
    23461 tatcacgtaa atagtatatt ttttatacta aatttgtgaa atatttatta aaataatact
    23521 aaaaattgta aatttataaa aattaacaat ctcttatatt atacttataa agaaatcaaa
    23581 ttgcaacttt attcattctt ttacacattg aaaataagtt attataattt ctaaataaat
    23641 atgctttttt taaaacatca ttctagctgt tttgtttcat atttaaaaag agtacttaat
    23701 ttgtaaatac tcttttttaa taactttata ttttagcaaa attacatgca ggataagtac
    23761 atacatcaca gttcatacaa aggccaccat gaccatatct agctatatca tacttatcaa
    23821 gcttttcatc aattaaaacc ctagataaaa ctatatcaaa tactgtagtt ttgtgataca
    23881 ttgcgcatcc tggtataccc ataattggaa cttctccata ataagcaagt aaaaacatag
    23941 ctcctggtaa tattggtgaa ccatatgtca ctaaatcagc tccagtttct tttatagctg
    24001 ttggcgtaac atcatctggg tctacactca tcccaccagt acaacagata agttctgctc
    24061 cttgacttat aaattctttt attgcactct ttataatctc tttatcatct gggcatatag
    24121 tctgtcctat aacttcacaa ccaaagcctt ttactttttc ttctattact ggtccaaatt
    24181 tatcaacaat tctgctataa aacacttcat ttcctgtagt aacaattcct actttcttag
    24241 gtttaaaagg tactacattt actatttttc tattaccaac tacttttttt gcatcttcta
    24301 attttttctc atctataact aaaggtatca ctcttgttcc agctactttt aaacctttat
    24361 ttactataaa attattatga agagtagcca tcattatttc tccaacacaa ttcaaatcaa
    24421 aaagtgcatc aacatctatc tttaatagac catcacatgc tgctataaaa tctattttcc
    24481 cttcttttat ttcactaaaa tctaaatttt ctccagcagt tagttctttt aaaaacaatg
    24541 ctccttcatt ttcatgaacc attccttctg acttttccca tacatacaaa ttatctttac
    24601 caagagataa taacactgga atatcttctt cttttactat gtggcccttt ttaaattttc
    24661 ttcctttaaa ttctccaggt attatttgag ttatgtcgtg acataaaact tgtccaacag
    24721 catctatagt ttttattagc ttcataattt acccccaatt ctctcataat ccatttttgt
    24781 atcgatatca aataacattt ctttaggaac ttctacatac tttatttttg atgaattttt
    24841 taaaatggtt ttaccttttt catcttcttt aagtgccaac aattcttctt tcttactata
    24901 tgggaatatg actggatttc cacacatatc tttgtatctt ggaattacta taaaatctct
    24961 atctaattta aaagtattta taattttatc tatatataag ttatcaataa atggctggtc
    25021 tcctacaaag aacatataac catcacattt tgagctattt aaaactccta attttataga
    25081 ttcactttga cctatatttg catttttatt atctatatat tcaaaaccat atttattaca
    25141 caataacaaa acctcttcat attgacttac tactacaact tgatgaaaat caatcttgct
    25201 aacttctcta aatacatgtt ctattataga acagccttta taatttaata aaagtttatt
    25261 tgctcccatt cttctggcaa agcctgatgc cattatcact gcatttatca tatatatcat
    25321 ccttggtatt tataatatat tgatagttct atgattttta taatatctct attgatatta
    25381 tctatatata taatatcata taaactattt taatacttca atatcttaca caaaattttt
    25441 gttttacact tccataaaat atctctattt cactttcata ttctttaata ttttctatta
    25501 attttatagc taaattttta tatttttcat tttcaacttt attaataaat aatactcttt
    25561 ttccagaata atttttaaat aatccatttg gatttaatac tatatttttt aagttttcaa
    25621 tacttactgt agaactacta ttaatactac ttgtatctaa atttgctatt tttttaaata
    25681 tttctactct atgtatattc tcttcattaa tattcattcc aaatgatgtt atatctaata
    25741 tccctatggt ttttgtagtt ttatgatata ctactggttc tttagcattc caacctttaa
    25801 gtttttttct ctttgagcca tctccctcta ttaaaactaa atcaaattgg tctacaattt
    25861 catccaaaat agaggaatca atacttacta atttattttc attatttata taactaccac
    25921 atactgttat tccatgacaa atagatggaa taacagttcc atctattgta ataattatat
    25981 tgtcataatc gtttggtaca tatattttag ttgttgttgt aagaagaact ttcaatttat
    26041 ctctataaaa atttgcaaaa tagtttataa acgaagtttt tcctcctgct ccaactacag
    26101 ttattatttc attcttttct attatatttg atagttccac actttcacct cttataacca
    26161 gttttctaat ctcttaatta ttatagttag acctttttag taaaagtgat ttttgaaatt
    26221 ccataaactc gaaagtaaat agattctgtt gtaaacaaca aggcggatcc aattcgccgc
    26281 taagctgtaa gcttacgcta tcagtttatc ttttaaatat aattaagata ttcttactct
    26341 ctgtggttgc tttaatattt gaggattatt ctgtaaatta gataaattaa ctacttgtga
    26401 tttttttagt atattaagac tcgcatttac atctgcattt ataatgattc caacactgga
    26461 tttgtactgt ccacgcgtta ttctttcccc tttaaagtca tattttttaa cattgtccac
    26521 attgatatca ggtagaaaat catcagcaaa aaaatctgat ttactagtat agctttcttc
    26581 ttgcctaata aaattgatat tttttatttt acacagatat tctaattttc cttttatctc
    26641 tccaaatggg attcttataa aatttctata tatttttctc tccatattat cacttttttc
    26701 taacttagca ttatatccta caattaaatt tccaatatta ttttttacac aataatcaat
    26761 tatataaaaa caggttttat ttacatagtc tttaacttga ttatttctct tattccaaac
    26821 tttaaattgt tttcttgtta ttgcagttat attctttttt aattttaagg cttctatttt
    26881 atcattctgt ttgtttgccc actgattaac agattttagc ctttttccat ctatgataaa
    26941 actatcacct aaattggtaa cacatgtaca taaattttct aaacccaagt ctatagctag
    27001 tgcatttttt tgatttgaaa cttcctctga ttttggtatt tcataacaat actgaatttc
    27061 aaagaaccta gctttatgtt taggtattat tcttatttct ttaatttttt tctctagtat
    27121 attttgtgga acttttatac ttactttccc atatagtttt ttaaatgctg gagacatagg
    27181 tacatttaaa atcccatctt ctttgattct tatttgacct attattatgc taaaaaaccc
    27241 ttctttatct aaatatttag gtgattttat ccctctaaag tcatattttc cttctttggc
    27301 taatttaatt aacccaaaaa atgacctaaa cgaactgtct acttctttta atatttgttg
    27361 cgctatatta ctattgagaa gcttatagtt ctcattttct ttgcatacat gataattagc
    27421 ttcatagtta agatgttctc cttgattgaa ataatgctgt cttacattat atgtaccaac
    27481 attatataag ttcttagcta agaagcacat ctctcgtaga gcttgatatt cttttttagt
    27541 taagtttttt aattgttgtc tctgagttcc atagtgtata ttttttattc tgtttgacat
    27601 gaactcacct cctttagaat atactctact agacattata tacggtattt ttacaaaaca
    27661 ccatatataa tgtccattta attactttat tttacaaaat aatttaaatt tacttgcttt
    27721 tatacacatt attatttatt ataattgttt attattattt ttcataccca tataaacttt
    27781 ttcaaaagtc ataggaagct ctcttaaacg aactcctgta gcattaaata ttgcatttgc
    27841 tatagctggt gctggtgtat gtataactat ttctcctaat gatttagcac cataaggacc
    27901 tcctgggtcg aaattctcag caaaatctac caatatattt ccaacttctt tttttgtagg
    27961 aactttatac tgtaggaaac tatctgttaa taatctccca tctttatcaa atatagggtc
    28021 ttcatacatt gcaaacccta tacccatcat cacaccacct tctacttgta ttctagctaa
    28081 agttggattt ataactgttc cacaatcagg tacacatgca aaatttatga ctttatattc
    28141 gcctgtattt ttatctaatt ctatctctgc aaatcctgct ataaatggtt ttattttttt
    28201 caacatgcca aaagattctg ttgcttccaa aactactggt acatcatcac tagcaaatcc
    28261 tattcctcca acagattctc tacccaattc ctctaataga acaaacttgc tattatcgtt
    28321 tttaacaaaa accctgtctt ttcccaactc taaatcttct tttttaaatc ctagctttga
    28381 ctctgcaact gaaagtaata tatcttttaa tttatttgct gtctttattg ttgcatttcc
    28441 tgtaactgtt gttgtacaag aagcaaatgc tcctgtatca tagggacata agtctgaatc
    28501 acctgtataa atagaaattc tatctacagt agtatttaaa gcttccgcag caatttgagc
    28561 ttgaattgta tcagcccctt gacctaagtc agcaagacca gataatagtt tatatgtgcc
    28621 atcttcatcc attcttatag tgactgttgc catatctaca cgcggtatac cagaaccatg
    28681 atttgctact gccataccta ctgctcttac tttgttgttt cctatatctt tacatggata
    28741 tttatcatcc caattaatta atttcttgcc cttttctata cattgtctca gctcacaact
    28801 taatatttcg tcttcagctg cttttccatt ggtataccta tctattatat ttttaattct
    28861 aaattcaagt ggatccattc caatcttatg tgccaattca tcaatagcac tatctattgc
    28921 aaatattccc tgtgtagcac cataccctct aagtgctcct gctggtaacc tattagtata
    28981 tactgttcta gcattaaatc taacagcatc aatatcatta tatattggta aggtattgtg
    29041 tccagattcg gaagaaacag agacaccatt cccaccataa gcacctgtat tgtttaatga
    29101 ctttagctct attgctttta aatttccttc tttatctgca ccaactttaa catcaaaaaa
    29161 catttcatgt ctactattag aacctacaaa agtttcactt cttgtaaata caaattttgc
    29221 tggtctacca gttttcatag taacaaatgc agtaaatata tctgttgctg ctatattctt
    29281 tcctccaaat ccaccaccta ttctaggttt aattactctt atttttgaag atggtatacc
    29341 caatactttt ctaagtattc tctttacatt gtgtggtgat tgtaatgatg aaactacaac
    29401 aattctatca tttatatctt tatatgaata tgacctatga gtttccatca tgcaatgcga
    29461 ttgactttgg gttttgtatg aagattcaac cacaaaatca ctttctttaa gcttagcttc
    29521 aacatctcca aattcaaaaa attcttttga tactatatta ttttttgcat ccaatccaaa
    29581 aggatatggc tctactatgt ctttttctgg atgtataaca acttcatttc cttctgcttc
    29641 tcttgcatca aacacaggct ttaaaacctc ataatctacc tttatcattt ttaaggcttt
    29701 aagtgccgtt ttctcatctt cagcagccac taaagctact tcatctccaa taaatctaac
    29761 tgtatcttca agtaaaactc tatcataagg tgatgcttct ggttctgttt gacctggcac
    29821 acaatatctg acctgtggta catctttata cgtataaata cccacaaccc ctggaatttt
    29881 ttcggctttt gatgtatcta tattctttat cttagcatga gcatgtggac ttctaagtag
    29941 ttttaatgtc aaaacatctt tatgcataat gaggtcttca gtatatagag gttttcctgt
    30001 gacaattgct gtactatcta tctttcttat ccccttatta atcattatac attccctcct
    30061 caatagcatt atacatcctt ttaactaatg cacttgccat atcctttctg tactctttac
    30121 tagctctcat attacttcca taagtaagtt ccttactagc aacaatagat gccttatcta
    30181 tatctttttc tttatttaat atattactcg cttcaagtgc taatctagct ttttgaggtc
    30241 ttgccccaat tgctattttg taagtatcat tttcttttag cattgctgaa tttaatacag
    30301 aaaaatctcc tgtgcatttt cttattgagt caaaaacagc tattgcatct ttttttggta
    30361 gtattacttc gaccaatata tccctactta attcactatc caaaaactca cttaactcta
    30421 gtattccttt attgtataat ctcaccttcg ctccaacaac caaaagcgct ggtattaagt
    30481 cagaaaagcc atactttgaa aatacacttg caccaattct agccccttgt ctaaattgaa
    30541 caccaactat attagaaaca gcgcttgaaa gtattccact acagtaattt tttatagttt
    30601 tatcaatttc taaacttcta agtgaagtat ctgcacctat caaaatattt ttttcatcct
    30661 cttttatata atttagattt atatctttta aatctatcgc tattcctata tttttattac
    30721 ttaattttat aaatccacat ccacctaaaa taacattatt ttttttagaa attaaaattt
    30781 tatatgcttc ttctaaactc tgtggaactg aatattcctt tatagtaacc atcttatccc
    30841 cccatcatta acttattaaa acacaaaagt ttttaataaa tcttttattt atatttataa
    30901 ttgtagtata tttaattcac atatattaaa cctttacatt ataaactttt atttttatcc
    30961 tcaaccttat cacttttatt tccaggaaat tttaccatat ataaagcttg aattattatg
    31021 gctaccattg ctcctgaaac tacacttgaa tctgcaagta atgtctctat aaattttgga
    31081 aatttttcaa gaacttgagg agctaccatg aatccaatac cagaagccat agaaagacct
    31141 aatatcatgc tattttcttg attaaatcca tccatactaa caagtcttaa accagatgtt
    31201 gtaagagttg aaaatataac gagtgttgct cctccaacca cacagggagg aattgttctt
    31261 attaaattag ctaataaagg cgatattcct gccaaaaata atccaattcc tccaatagct
    31321 agtacaaatt tacttacaac ctttgtattg gaaacgattg cagaattttg tccaaacatc
    31381 cccattggag ttgcagaaaa taatgcacct attgtagaga gtactccatt tcctaaaaca
    31441 gcagaagaaa gttcttcatc tgttacttgt ctattaagcc ctcctaccgc agaaagtgta
    31501 caagcaccca ttatatcagc tatctcaact aaatatatta ttgaaaacat tacaacaact
    31561 tcaagtctta tatctagccc aaaagcaagt ggttttggaa gcgcaaccaa agtaaactcc
    31621 tgaattgaag aaaaatttac tatacccaaa attaatgata aaatgtatcc aaaaattatg
    31681 ctaactaata ttgatgcatc tttaaataat ccttttccat acttatttaa tcctaatatc
    31741 ataagagata cagatattcc aactataaaa ttaattggat tcccaaaagt ctcattgccc
    31801 atacccccag ctaagttttt tatagctatt ggaaaaagtc caagacccat acatgcaact
    31861 attgttcctg atacagctgg agtaaatata aatcgtattt ttttaagacc aaaaccaatt
    31921 ataattatta ctataccagc tattatttga gagccaaata atactggtag tccatactta
    31981 cttgcaacag ataccccagc tcctataaac atagctgtag ctcccattat aactggtatt
    32041 cctcctccta atttaaaacc tttaaacaaa ggtattggat ataattgtaa taaagttgat
    32101 aatcctgcaa aaactaatgc ggcttgaatc aataatgttg acatcttatg attaagtcca
    32161 gctgcatttg caatcaatat cgctggtaaa caactaccta ttaccattgc cattaagtgt
    32221 tgtaacccta aaggtattgc ttcctttagt ggtggtattc cgtctaattc aaattttgat
    32281 gtactttttg ctttcataat aaaaactccc gttacataaa acttattgaa ttatataaat
    32341 acttaaaaat ctaatatatt aaattaatga tgttcataaa tatcaactat actccaacat
    32401 atatttaagt ttttagtctt aaaaaacaga tgcaataaat ttaattatta acaaatacac
    32461 gtattcttta attttgtata cattatatac catgctttac aaatataaga gtattatagc
    32521 tatgaatttt taatttttta tagtaaattt aattcaattt aaatctttca atcttaatca
    32581 ttataagtta taagttttca taagtattta catgtttcaa gttttaaaca tttacctata
    32641 aacgctactt tatagatgtc acttcaaatc cagtgacctc atactttcat atgagcgtag
    32701 actatttctt catcttcagc attacctgct aagagcaacc cgtttccact atcaatcgct
    32761 tatagtgtac tctctctcga gatagtcgtt tgaccttcct atttctaggc ttggcgacca
    32821 aatgtccatt tttaagtact taggctctca ccatatacca tcctcactat tgtttctggt
    32881 tttcaccaca ttcataaaag tttctttcta tcttttattg tagttgcaag gctttaggat
    32941 ttactggttt taaagttgtg tcctacatag atttctctat gtacggggcc aaaagtacaa
    33001 tttatttaac atactttgct ttaccccctg taaataatat tatttactct ttcactgttt
    33061 ttgttattaa attaattaaa tatcaattaa tctaagcata aaataattat ttaatttaaa
    33121 ctcaaaatat ttatgaatat tttataatat aattctcata agacagaaaa acttaataag
    33181 ctatgtatct cagttgtacc aatgtactct cctttactat atttgtatct gcaacataaa
    33241 atatacaagt ttctatctat ttatagatat atatatctat aagcaaaata gtaatattat
    33301 ttttatagta aaaattttat tcatattact atttataatt tttatagagc aattttagtg
    33361 ccaatatttg catacatttg tatagtataa aatttttatg ttctcattga taaaatttta
    33421 cttatttcaa gcgttctatt agattttatc atctaaattt taggttttat ttttataaat
    33481 taattgtatt tatataaaaa aaaagaccta aaaacaaatt tttaacggtc ttttctcaat
    33541 tttttatttt catttctaaa atttataata aatagtaaga acactataaa ttcttttata
    33601 taaaattctt agtttgtgga tgataaatta gtgaattaaa atactacttt actgatttta
    33661 gtttgacaat atcactccaa tttttactag tggctaactc tactaaattt aagtctcttc
    33721 ttatttcagc tatatcctct tttatagaat tagtgtcacc ttttatatat gctatgttat
    33781 gttctatatt atcatgagta gctttattta tttcagaatt atgtaataaa cttcctaaaa
    33841 tattattctg ttctatctgt atctctttca ttgatttatc ttcacattct agactatcaa
    33901 tcttaatttc aattccatat aatttttcat ttgctatgga tacctcgtcc ttaattccat
    33961 tcatatcttc attaatgtta tttattttat tttgcataaa gtttacttct gatttaactt
    34021 cggaaacatc ttctttcatt acagttattt cttgtctcac ttctgacatg tcttttttca
    34081 ttacatttat ctcttgtttt acctctgatg tatcttcttt cattacagtc atttcttgtc
    34141 ttacttccga catatcttct ttcattatat ttatctcttg tcttacttcc gacatatctt
    34201 cttttaatac atttatctcc tgttttactt ccgacatatc ttctttcatt acagtcattt
    34261 cttgtcttac ttcggataca tcctctttca ttatatttat ctcttgtctt acctctgaca
    34321 tatcttcttt cattacagtc atttcctgtt ttacttccga catatcttct tttaatacat
    34381 ttatctcctg ttttacttcc gacatatctt ctttcattac agtcatttct tgtcttactt
    34441 cggatatatc ctctttcatt atatttatct cttgtcttac ctctgacata tcttctttca
    34501 ttacaatcat ttcttgtttt acttccgaca tatctccttt taatacattt atttcctgtt
    34561 ttacttccga catatcttct ttcattacag tcatttcttg ttttacttcc gacatatctt
    34621 cttttaacac atttatttct tgttttactt ccgacatatc attttttatg ctaactacat
    34681 cttcatttat agtatataat aactctaaaa tcttctcttg cacttgcatc accatcccta
    34741 ttaattatta taacatttta cacaaatgct atacaatgtt aacatatata tcagaattat
    34801 taaaatttta attaaacaaa attgattcac tttattactt caaaactaat taatatctaa
    34861 aataactaca aataaacaaa aataaggtca acaacaaaaa aggcttatct ctttgagata
    34921 gcctttctta tttttattta aaactttgtt ttttataaaa taatctatac attcagctct
    34981 gaacatgaaa tttttcttcc aagtgctttt ggtttacaaa ctgatataca aagtccacaa
    35041 tatctacact cttcttcatt caattccatc ttatcattca attttctcgc ttgatatgca
    35101 caaactctct cgcacatctt acatttagta catttttctt catcataagt aaactcaaag
    35161 ctaagtcttt cattaacatc tcccatatga ttatgtacta taccacaagc attttctata
    35221 gtattatatc ctaatctatt aagattagat tttaaatcat catgtatttt agatattact
    35281 tgtgcgcctt ttaaaattac tactgaacat ataccaacaa agtcagcacc tgccataatc
    35341 atttctaagg catcatctcc acttacacat ccacctaagc caattattgg tatattaact
    35401 tgttttttta tttcatatat tctctgtaaa gttattggct tgatagtttc tccacttaaa
    35461 tatcctattc catttccacc taataaaggt tgccctgttt ctatgtctat tctaaatgct
    35521 ggtcctacag agtcacaagc agttattgca tctgctcctg cttcttctaa ttttttagca
    35581 aaatcgccta tctcatctat cataggagga agtttaacta ttacaggtat atttactctt
    35641 tcctttgcat ctttaagcat tggtatcaaa tctctatagt cataagatac taattcaata
    35701 aagtcagctc ctgcatttgc aactttctct actaattcgc tactttcttc aattgtatga
    35761 cctattgaag ctatacagac agtcccatct cttttcattt gtggtatttc aaaatctatc
    35821 cattgctctg gctcaaaatc tgtccatttt tcattattta aaagagcatt agctgtattt
    35881 ctaaccatat gtggtgctgg atttatagct ctttcttttc ttatagtctt agtcacaact
    35941 gcccctgctc ctgcatcact taatatcttg catccagctg cactatatgt taatggtcct
    36001 gaacctatta taagtggtga ttttaattct ttacctaaaa aattatacat tatctgcttc
    36061 cccttttcat atatatttac attcgttaaa atttccaaaa ttcacaagca acttctttca
    36121 tttctttaac aatagctttt ttatctaaat caactaactg tttttctttt aaaatatgtc
    36181 taccatctat gtatacattt gatataaatt cttttttacc ctgaactact atctggtcaa
    36241 atatattatc aagagtcaca ggagttttaa attcatcttc cataactatt atatcagcct
    36301 tattaccaac tgctatttta ccagaatttt gaagtcctaa tacatgagca ccatgctctg
    36361 ttgccattct aaatactaaa tttgctggca ttactgatgc atcttcttca actgatttat
    36421 gcattaaaaa tgcacctctc ataacttcaa aaaaatcatt tatatatccg tcagtaccca
    36481 gtgccacttt tatacctttt ttaatcattt ttggtactgg agaaaaacct ccacctactt
    36541 cacagttgct aagaggcata tgaaccactt ttacaccttt ttcttttaat atatctattt
    36601 cttcatcatt tacttttaca cactgagatg ctaatacagt ttcatccaat atacctaaat
    36661 cattatagta gtcaactgct tttttgccaa aatgtttttc agcatagttt ggctcatata
    36721 tactctcaca taaatgaaat tgcattggtg catttaattt ttttgcatct tctttagctt
    36781 tcttaataaa tctatcagaa catgtgaagc ttgtatgtgt acacattatt ccatttatta
    36841 atttactatt ttctctactc cactttatta aattagaatt ttcatctaaa catctaagtc
    36901 cattttcaaa gcttattctt tcacaacttt ctaaggataa aatcgctttc atgcctattt
    36961 cttctagtat ttttccttgc tcaattaaag ttccttcttc tatatttgga gcttctaggg
    37021 tatcacaaaa agctaccaca ccagattcta taagttctat tgctgatgct ttagttgttg
    37081 ccttcacttc tttcaatcca attctatttt ctataaatgg ccaccaatag tcatttaaga
    37141 aaccttcaaa gtcggtaaaa tgtacatttg ccggtattcc cctagaaaga actccatatt
    37201 gatgcatatg accattgacg aatcctggca ttattatagc atctgattta tctattattt
    37261 cttcaatatt tttatatttt atttcaactt cttcatttgg caatatatct tttataatgt
    37321 cattttctac tacaacagca gaatttgtat atatttcatc agcagaagat attaagtatt
    37381 tacttttaat agcaatcatt tatttacctc cctgatttaa ttttttcatg gccatataaa
    37441 ctttctcaga agttatagga agttctctta tacgaactcc tgtggcattg tatattgcat
    37501 ttgctattgc tggtgcaggt gtatgaataa ctatttctcc taaagattta gctccatatg
    37561 gacctgttgg ctcataatta tctacaaaat caacaattat gtttcctata tctttttttg
    37621 ttggaacttt gtattgtaat agattagaag tttgaagctt tccatcagaa ccatgtctag
    37681 ggtcttcata cattgcaagc cctatacctt gagttacacc accttcaact tgtattctag
    37741 caagtgcagg attcataacg gtaccacaat ctgtagaaca agcataattt attactttaa
    37801 actcccctgt tgttttatct aactctattt cagcaaatcc tgctaagaat ggtttagcac
    37861 tctcttgtat accataagat tcagatgaat ataaaacttc ttgatttcca ccaccaacag
    37921 attcttttcc taattcactt aacaaaacaa acttctctac atcttcacta taacaaactc
    37981 tgtcttcata tagtattaaa tcatctattg gtaaatcgag tttcttctgt gctactttta
    38041 atatcttttg ttttaaactt tcagcagatt ttattgtagc atttccagtt acataagtag
    38101 tacaagaagc gtatgcacca gtatcatatg gacacatatc tgtatctcct gtataaacgg
    38161 aaattctatc aatagatgta ttcaatgctt ttgcagctat ttgtgcaagt attgtgtcag
    38221 aaccagttcc taagtctgaa gacccagaaa acaacttata agttccatct tcatccatcc
    38281 tcatatttac tattgccatg tctatgttgg ctataccaga accatgagtt cccacagaca
    38341 ttccaacagc cctcacctta ttgttgccaa tatccctaac agggtacttt tcatcccatc
    38401 caataagttt tttacctcta attatacatt catcaagtgc acaacttctt attggaaaat
    38461 tcattattcc gccttcagtt tgttttttta taatattctt taactttatt tctgttgggt
    38521 ccatattaag tttatgcgct aattcatcaa ttgcacaatc aagtgcaaat gtaccttgtg
    38581 ttgctccata tcctcttaat gctcctcctg gaactaaatt agtatataca gttctcccat
    38641 caaattttat tgcaggtaca ttattatatg ttggaagtac attatgacct gattcagagc
    38701 aaactgaagg tccattatca ccataagcgc ctgtattgtt caatgctttc atatctatag
    38761 cttttatgtt tccttcttta tctgccccaa tttttatatc aaaaaacatc tgatgtctag
    38821 tattagtcat tgcaaaagtt tcttctctag tataaattag ttttgctggt ctttttgtca
    38881 tccaagttac aaatgatgca tatatctctg ttacagctat gttttttcca ccaaaacctc
    38941 cacctattct tggctttatt actctcactt ttgaaagagg tattcccagt gctttagaaa
    39001 cctgtcttct catatgataa gctgattggt ttgcagaagt tactactaat cttccattcg
    39061 tatctatata cgtataagct ctatgagttt ccatcataca atgggcttgt gattgagtca
    39121 tataagaatg ttctactatt acatcacttc ctttaaactc tttttctaca tcccctttac
    39181 ccattttttc tctcgataca atattttttg tagcatccaa tccaaaatca aatggacaaa
    39241 acaaatcatc ttcttcatgg attaaaattt tatttccttc cgattctctt gcatccaata
    39301 taggagtcat tacttcatat tcaactttta taagtttcat agctttaata gcagcccttt
    39361 catcttcagc ggctattata gcaacttcat ctcctacata tctaactaca tcttcaagta
    39421 ttaattggtc atgaggtgat gcttctggat atgactctcc aaccattgaa tatctataat
    39481 ttggaacatc tttgtaagta aatatgttta ctacaccttt tacctttaat gcattttttg
    39541 tatctatgtt ttttatcttc gcaaaagcat gtggacttct aagaagctta attgttaaaa
    39601 catctttatg aaaaattaaa tcttctgtat atattggttt tccagtaaca atggcttcac
    39661 tatctatttt tcttacctct ttatttacca tcacactcac ctccaatact attatacatt
    39721 ctcactacta aggcttttgc catatctttt ctgtattctt tacttcctct tatattactt
    39781 ccaaatgaaa gttcttcact tgatacttca ccagcttttt ctatatcatt ttctaaactt
    39841 aatatttcac ttgctttata ggcaattttt gctcttcttg gtctagcgcc tatcgcaatt
    39901 ttataaatac ttccttcttt taacattgct ccatttaaca ctgcaaaatc acctgtacat
    39961 tttcttatag aatcaaatac acctatagca tcttttttag gtaatataac ttctattaat
    40021 atatcttttt caagctcact ttctaaaaaa tcagctaagt tcataacccc ttttttatat
    40081 aacttaacct ttgcatctac cactagtaat gaaggtatta aatctgaaaa tccatactta
    40141 gcaaatacgc tagctcctac tctagctcca cttctaaatt gaactcctac tatattagaa
    40201 acagcgtttg aaattacccc attacagtaa tttttaataa ccttatttaa ttctaaagtt
    40261 cttaaacttg tgtctgctcc aattaatatg ctatcatcag tttcatttat ataatctaaa
    40321 gctaagtctt ttaaatctat tgctgagcca atatttttac tacctagttt taaaaatcca
    40381 catcctccaa gtattatgtt gttttttctt gatattagca gttcataagc ttcatctaaa
    40441 ctttttggaa ctacatattc ctttatagtt atcatactct ctccccatat caacttaatt
    40501 ttcacttaaa agtctaagtg tttaaatatt tatgtattgc tcttaattga cctttatatc
    40561 ctgtacatct acaaaggtta ccatttacat aatctataac ttcttcttct gttggatttt
    40621 gaagctctct tttcattgcc aaaaccatca ttataaatcc aggagcacaa aatccacact
    40681 ggtcagcccc ttcgtctgca agaaaatctc caaacttctt tgcttcatcc aagacaccct
    40741 caagtgttgt aatatgctga ccttctactc tagcagtaag ataattacat gaaagcacgg
    40801 ccttgtcatt taccatcact gtacataacc cacatgagcc tgtatcacac ccttttttaa
    40861 cacttacata tccattttct cttaatgtat ctgctaaaaa atcatctggt cttatactta
    40921 attctttttc tttattattt atggtacatt tgataatcaa ataaatcacc tcaaaactca
    40981 tatttttaag ctaaagtttt actatacttt tttatgaata aattacattt attaataaag
    41041 gctattccat ttatgacctt ccattctatt ttttatagtt cctcttatac aaagtctctc
    41101 agctgtatct tgagcatctt ttaatgcttt ctcttcatta gctgtcaata tttttgaatc
    41161 ttccattatt atatttccat ctactataac actctctata tttttcatac tagaagaata
    41221 tactaatgta gataccggat tatgcatagg tatcgctttt ggtgaaagca ttggattaaa
    41281 tattagtaag tctgcttttt tacctatctc caatgaacct atctcatctt ccattccaat
    41341 agctcttgct ccatctatag ttgccaactc aagaactttt tctgccgaca ttgctaatgg
    41401 gtcacactta ttaactttat gttgaagtgc tgttaatttc ataagttcaa gcatatcttg
    41461 agagttatta ctagcggctc catctactcc taaactaact gttattcctt ttttaagcat
    41521 ttctggcact ggtgcaacac ctgaagataa gtacatatta cttgctgtat tatgagacac
    41581 tttcatatcg tattttttag taagttccat atctttttca gttagataca cacaatgtac
    41641 cataagcaca tttggtccaa gtattcctaa tttttctaaa acatctatgt catattgccc
    41701 atgcaattct tttgctgctt ctctatcaaa aggagtctct gatatatgta ctgtaaataa
    41761 agcatcatca tattctttta ctactctcca tagcatttca agcatttctt gagaatttga
    41821 ccatattgct gcaggagcta ctcctatttt gattcttcca ttttctgtat tatggtgttt
    41881 ttcaaacaat cttcttacat ctttttcaac cgtttctaca tcttgcatta ttccaggatg
    41941 aactccaaat tgagctcctg tattcataca tcctcttcca agtattcctc ttattcctaa
    42001 ttctttataa gcatctatta ttccatcaca aagacctggc ttactatgag gatacatgta
    42061 gtcaaccatt gttgttatac cacttctaag tccttcaata catccaagca ttgcagcatc
    42121 ataagtatct tttggctcta aatagttagc tgctggaaat gtcatagttt ctaaccagtc
    42181 tttaagtacc atatcatctc ccaatccttt taataaagtt tggaataaat gattatgtgt
    42241 atttataaaa cctgggaata aaactttacc tttagcatct ataatttttt taacatcagt
    42301 atatttactt tctatctctt tagaatttcc aatatcagca atcttgttat cttttactac
    42361 taatgcccca tcaaaaataa cttctctctc tttattcaca gttactatta tggcattctt
    42421 aataagtata tccatcattc ttatttcccc ccgcattatt tagtaatttt tcttttaata
    42481 tattgacctg ttggatgttc tttaacatta caactttgta ctacatgatt tcctctcaaa
    42541 aatacatctt caactttaca tttaacctta aatccttcat atggagtata atctacattt
    42601 tgtttttgag ttttataact aattgtttct tctttattga tatttaaaat gactatatct
    42661 gcatcacttc ctacagctat ctcacctttt ttaggataca ttccaaatat ttttgcagca
    42721 ttcgtagaag taacttccac aaacttattt gcacttattc tattttctaa gactccatat
    42781 gtatataaaa gtgctaacct atgttcaaca ccaggtgctc catttggtat cttactaaaa
    42841 tcatcaattc ccaagtcttt ttgcccctta aaattaaatg aacagtgatc tgtgccaact
    42901 gtttgaatat ctccactaga caatccttcc caaaggtaat ttatgtcttc tttttttctt
    42961 aaaggaggag acattacata cttagcaccc tcaaatttat ccttattata taaattgtct
    43021 tctaataata gatactgtgg gcatgtttct actatcatat ctatattttt ttctctagcc
    43081 atttttacag tttcaagtga ctccctacaa cttaagtgga ctacatagga ttttgaatta
    43141 gataatttag ttatatcagc aagtcttgaa acagactctt tctcaactat gttaggtctt
    43201 gtaagtggat gatattttgg ctccaaatta ccattttgta tattttcttc tatcaataca
    43261 tctagtaaat ccccattttc acaatgaaat ccaactatac aacctaaatc ccttgctttc
    43321 tttaaaaccc tgtatattgt tccatcatct actttcatgc catcatatgc catatacatc
    43381 ttaaaagata ctatcccatc tgaaataagt ttttccatat gattaaatgt atctttattt
    43441 aaacatgtaa ttgtcatatg aaaaccataa tcacaataac aatttccact tgctttttta
    43501 tgatatgctt ctacaccttg cagtaggttt tccccctcat tggcttctgc aaaatctagt
    43561 attgtagtag ttcctccagc tatagctgct cttgttcctg tttcaaaatt atctgcagtt
    43621 gtaatagaac ctgcattcat atcaaaatga gtatgtgtgt ctattccacc aggaattaca
    43681 attttacctg tagcatcaat aaccttatca ccatttattt ctaagttatt tcctatttca
    43741 actatctttt catcttctat ccttaaatca cttatataag tcttatcaga ggtaattata
    43801 gttccacctc ttagtattgt tcccatatat acctcctaac ttttaagctt taaagtattt
    43861 tctaagtatt gtaaaacttt atttaaaatt gtattttcta aaggtgctat attcagcacc
    43921 tctagactat atttaaataa tttattgtga actaaatatt attttccgta tacagatttt
    43981 aatattccat agtaaccttc tgttcctata aataactgtt cttgctctat atactcatct
    44041 attgtgtgag caagattttc cttagaagga ccaaatccta tagtttttat tcccgcttca
    44101 cctgcatagt gacttccatt cgtacagaat gagtattgag ttatttctga atctattcca
    44161 gcttctttta atcccttata tgctgcttga acaaattcat cttcttc
//
LOCUS       DAEMHZ010000015        43379 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000015 DAEMHZ010000000
VERSION     DAEMHZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.015"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            126..755
                     /locus_tag="KPK72_000732"
     CDS             126..755
                     /locus_tag="KPK72_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2487976.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(869..2383)
                     /gene="glpK"
                     /locus_tag="KPK72_000733"
     CDS             complement(869..2383)
                     /gene="glpK"
                     /locus_tag="KPK72_000733"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2487977.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2627..3169
                     /locus_tag="KPK72_000734"
     CDS             2627..3169
                     /locus_tag="KPK72_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2487978.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3183..5675
                     /locus_tag="KPK72_000735"
     CDS             3183..5675
                     /locus_tag="KPK72_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2487979.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGVKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGLDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6096..6494
                     /locus_tag="KPK72_000736"
     CDS             6096..6494
                     /locus_tag="KPK72_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2487980.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6711..6920)
                     /locus_tag="KPK72_000737"
     CDS             complement(6711..6920)
                     /locus_tag="KPK72_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487981.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(6952..7161)
                     /locus_tag="KPK72_000738"
     CDS             complement(6952..7161)
                     /locus_tag="KPK72_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487982.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7460..7879
                     /locus_tag="KPK72_000739"
     CDS             7460..7879
                     /locus_tag="KPK72_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2487983.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8107..8562
                     /locus_tag="KPK72_000740"
     CDS             8107..8562
                     /locus_tag="KPK72_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487984.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8783..9043)
                     /locus_tag="KPK72_000741"
     CDS             complement(8783..9043)
                     /locus_tag="KPK72_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487985.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(9306..9659)
                     /locus_tag="KPK72_000742"
     CDS             complement(9306..9659)
                     /locus_tag="KPK72_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487986.1"
                     /translation="MFKFDKNKIEQIKQGIKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9676..14907)
                     /locus_tag="KPK72_000743"
     CDS             complement(9676..14907)
                     /locus_tag="KPK72_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBG2487987.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSSGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINASKEIFVSGCNLAGQLGSFFHTTFLYEFSKVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNIKLSDNFQDYKKIELTDNNMFIVMNDGSLYACGLNNAGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGSSTFVLKNNGTLYSCGLNSSGILGLKDNTNRNIFTKI
                     EIENIKEFCVEGNYVIALNHSKELYGWGNQSYIVYGDNRNYPYKDTRVSNVEKIATWH
                     DTLYILDSTGVAKTIGYSCNGSGGYPAPSTSSSYQSKGYNAWNTSYRTLEFYNTAQTK
                     LINLFAFYHGCMFFDESDRAYCIGENNMKFTSSSQITPESELRFSSNSGIYHTYSDGV
                     AYTCYQWTYKLIRCSVFDSSKSVVGNSKNILSLLKNNSTFRCTGSCLTYGQTNQNWSS
                     YLSDNCNGAVSLGNEFILKNYSGESVLKGYGKSNNGEFGSSTSISNASNYDTGLKDIK
                     DIIVKGNTVVVVDKNNNIYVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDI
                     KEITTSRNTMFIVKNDGTAYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDG
                     NTTFAIRNDGTLYSTGLNTKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDD
                     NTLYSCGENTHGQLGLGSESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCC
                     GYNNNSQLGMGNTTDQYSFIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGL
                     NSNSNQSSFSEIPISNVVKVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIK
                     KIASKAKDIGMNYRCGHYVSDNGDLYGTGFNDCGQLGVGNVTKRDTFIKTNTRVKKIL
                     PLEYANIAIKDTNDIYICGLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIK
                     NRHNFILLNNKIVIPTTKDIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLN
                     DGTMYGCGTNYHGELLQDLSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIG
                     KNSEYQLGIGHNNPVTELQRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALG
                     LGSDSENTIIKFFTKALTDIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEF
                     LKEFTIIPISEDVNTVKDVLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNK
                     DISRIEMLINGESVKSVSDLITEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFI
                     FKESTGNSIIKDSYVMIGNRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINK
                     TKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIK
                     RPSMIFRYDAENNEPQA"
     gene            complement(14929..15915)
                     /locus_tag="KPK72_000744"
     CDS             complement(14929..15915)
                     /locus_tag="KPK72_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2487988.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(15916..16614)
                     /locus_tag="KPK72_000745"
     CDS             complement(15916..16614)
                     /locus_tag="KPK72_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG2487989.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAAVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16611..17663)
                     /locus_tag="KPK72_000746"
     CDS             complement(16611..17663)
                     /locus_tag="KPK72_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2487990.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17656..18084)
                     /locus_tag="KPK72_000747"
     CDS             complement(17656..18084)
                     /locus_tag="KPK72_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2487991.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(18084..18410)
                     /locus_tag="KPK72_000748"
     CDS             complement(18084..18410)
                     /locus_tag="KPK72_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2487992.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18426..19955)
                     /locus_tag="KPK72_000749"
     CDS             complement(18426..19955)
                     /locus_tag="KPK72_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2487993.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVILSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(20237..20659)
                     /locus_tag="KPK72_000750"
     CDS             complement(20237..20659)
                     /locus_tag="KPK72_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487994.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(20659..23112)
                     /locus_tag="KPK72_000751"
     CDS             complement(20659..23112)
                     /locus_tag="KPK72_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487995.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFAP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23112..23279)
                     /locus_tag="KPK72_000752"
     CDS             complement(23112..23279)
                     /locus_tag="KPK72_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2487996.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23306..23752)
                     /locus_tag="KPK72_000753"
     CDS             complement(23306..23752)
                     /locus_tag="KPK72_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2487997.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(23866..24294)
                     /locus_tag="KPK72_000754"
     CDS             complement(23866..24294)
                     /locus_tag="KPK72_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2487998.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24308..25372)
                     /locus_tag="KPK72_000755"
     CDS             complement(24308..25372)
                     /locus_tag="KPK72_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2487999.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLAGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25377..25820)
                     /locus_tag="KPK72_000756"
     CDS             complement(25377..25820)
                     /locus_tag="KPK72_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488000.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVRDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(26069..26509)
                     /locus_tag="KPK72_000757"
     CDS             complement(26069..26509)
                     /locus_tag="KPK72_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488001.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(26614..26811)
                     /locus_tag="KPK72_000758"
     CDS             complement(26614..26811)
                     /locus_tag="KPK72_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488002.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            26980..27300
                     /locus_tag="KPK72_000759"
     CDS             26980..27300
                     /locus_tag="KPK72_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2488003.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27382..27888
                     /locus_tag="KPK72_000760"
     CDS             27382..27888
                     /locus_tag="KPK72_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488004.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            28589..29344
                     /locus_tag="KPK72_000761"
     CDS             28589..29344
                     /locus_tag="KPK72_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2488005.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(29487..29915)
                     /locus_tag="KPK72_000762"
     CDS             complement(29487..29915)
                     /locus_tag="KPK72_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2488006.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(30336..30536)
                     /locus_tag="KPK72_000763"
     CDS             complement(30336..30536)
                     /locus_tag="KPK72_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2488007.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(30719..31888)
                     /locus_tag="KPK72_000764"
     CDS             complement(30719..31888)
                     /locus_tag="KPK72_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG2488008.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            32124..32945
                     /locus_tag="KPK72_000765"
     CDS             32124..32945
                     /locus_tag="KPK72_000765"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG2488009.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDKMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(33021..34409)
                     /locus_tag="KPK72_000766"
     CDS             complement(33021..34409)
                     /locus_tag="KPK72_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG2488010.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVKFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(34544..35302)
                     /locus_tag="KPK72_000767"
     CDS             complement(34544..35302)
                     /locus_tag="KPK72_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2488011.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVAILVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     ASIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(35304..36020)
                     /locus_tag="KPK72_000768"
     CDS             complement(35304..36020)
                     /locus_tag="KPK72_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2488012.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYIFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(36022..36729)
                     /locus_tag="KPK72_000769"
     CDS             complement(36022..36729)
                     /locus_tag="KPK72_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2488013.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(36845..37381)
                     /locus_tag="KPK72_000770"
     CDS             complement(36845..37381)
                     /locus_tag="KPK72_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2488014.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(37483..38553)
                     /locus_tag="KPK72_000771"
     CDS             complement(37483..38553)
                     /locus_tag="KPK72_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG2488015.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(38546..39604)
                     /locus_tag="KPK72_000772"
     CDS             complement(38546..39604)
                     /locus_tag="KPK72_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG2488016.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(39591..39923)
                     /locus_tag="KPK72_000773"
     CDS             complement(39591..39923)
                     /locus_tag="KPK72_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2488017.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            40122..41243
                     /locus_tag="KPK72_000774"
     CDS             40122..41243
                     /locus_tag="KPK72_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG2488018.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELINNHYS"
     gene            complement(41371..43269)
                     /locus_tag="KPK72_000775"
     CDS             complement(41371..43269)
                     /locus_tag="KPK72_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG2488019.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        14264 a         7288 c         4834 g        16993 t
ORIGIN      
        1 aaatattatt ttaaaacatg cacaatattt gtacatattg tagacatata aacctataaa
       61 ttatacaata gtattgttaa gattatatat ttttttaaca taaattttgt attgggggaa
      121 aaacaatgga taaaaactat acgatgccaa tataccaaaa aatagcgttg gatattgcaa
      181 ataaaatata cacgggtgaa atacaagagg attcagtact atttggacgc tctgttttgg
      241 ctggtaaata caatgtatct ccagaaacga taagaagagc tgtcaaaatt ttagaggaca
      301 ttggcgttgt taaaagcata aagggtaaag gtgttattgt actatctcca gacaaagctt
      361 cttcttttat aaaaaaatat agagatatta caaatatatc atcatataaa tcaacacttt
      421 ataatttaat agatactaaa tctaatttag aaaatgaaat attagatact ataaataaaa
      481 ttttagacta ttctaacaga cttgaaataa tcaatccttt agtgcccgtt cagttcacca
      541 taaattctaa ttgcaaatat attggtcaaa cagctgcaca aactaaattt tggcaaaata
      601 caggtgctac aatagttgca attaaaagag gtgaagaact tataatatct cctggaccat
      661 atattgaatt tttagaagga gacattctct tagtagttgg tgaccaacac atttacaatt
      721 ctataccaat gtttttatat gaaaacgaaa aataaattat ataatttagt gtgattatat
      781 aaaaatgcaa gattaacaac aaaaaaagag cttcatattt tacataaagc tctttttaaa
      841 ttcaaataaa tttaactttg attaattttt aaatatccaa ttcatcttct ttttcccagc
      901 ttaaagctct cgatacagct tttttccatc ctttatataa tttacatctc ttctcttcac
      961 tcatatttgg attaaactct ttttctactg accactttga agtaatttca tttcttccaa
     1021 tatagaatcc aacagacaac cctgctaaat aagcagctcc taaagctgta gtttctacta
     1081 ttttgggtct atctatatct acatttaata tatctgattg aaattgcatt aaaaaattat
     1141 tattacttgc tccaccatca acttttaatg attttagttt taattttgaa tcgttttgca
     1201 ttgcttctaa aacatccttt gtctgataag caattgattc caatgtggct cttactatat
     1261 gttctttttt tgctcctctt gtaagtccta aaattgaacc cctagcatac atatcccaat
     1321 atggagctcc aagacctgta aatgctggca ctacatatac accattagta tcctctaccc
     1381 tatttgcata catttcacta tcttttgcag attcaattat ctttaattca tctctaagcc
     1441 attgaacaga tgctcctcca ataaatatac ttccttctaa tgcatatgtt acttttccat
     1501 caactcccca agcaattgtt gtaagtaacc catgcttaga tttaactatg ttactgcctg
     1561 tattcataag caagaaacaa cccgttccat aagtattttt tgcagtacct tcttcaacac
     1621 aattttgacc aaataatgct gcttgttggt ctccagcaca tcctgcaata ggtattttag
     1681 ctcctgataa catgcgctca tctgtatgac cataaacata gctagaaggt tttactaatg
     1741 gcaacatgct tattggtatg tccaattctc gtaatatatc ctcatcccat tctaaatcat
     1801 ttatattgta catcattgtt ctagatgcat ttgagtaatc tgttacatgt acttttccac
     1861 gagttaaatt ccatattaac catgtatcta tagttccaaa taataactct ccttgttctg
     1921 ctttttccct agctccttct acattatcta atatccattt tacttttgtt gctgaaaagt
     1981 atgcatctat taaaagacct gttttttctt ttattgattc ttccaatcct ttatttttaa
     2041 gttcatcaca tatttctgac gttctcctac actgccatac tattgcgtta taaataggtt
     2101 taccagtata cctactccaa acaaccgtag tctctctttg attagttatc cctattgaag
     2161 caatttcttc tggtcttata cctgctgttt ctataacttc tctcattacc ccactttgtg
     2221 acccccatat ttccatagga tcatgctcta cccatccagc ttttggataa atttgattga
     2281 actctttttg tgcaattttc actatttcgc cttttttatt aaatagtata gctcttgaac
     2341 tggttgttcc ttggtctaat gccattacat acttcttttc catcaccata ttctccttat
     2401 catataatat ttaatttatt aaaaagaaaa atttaatatc acatatttca ataaataatc
     2461 atacttaagt acaaatttaa tataatagat atttagtaag aagacactta ttttctatca
     2521 tatctatata ataatattat acattatagc ttacatattg attaataaat tttaaaggag
     2581 tataataaac agtgttcata ataattttaa taaaagaggt ggtaatatgc caaagatttt
     2641 agaaaatgta aaagaagata tattaaaagt gtctcgggat atgattttag aagaagatta
     2701 ctcgaatatt agcataagga aaatagctca aaggtgtggt ataagtgcag gtacagtgta
     2761 taattacttt aattcaaaac aagaaataat agaatatatc acaaaatctg aatgggattt
     2821 attgataagg cgaattgaat actccaataa aaacactgaa gattacataa aaaaactaaa
     2881 cattattttt actgaaataa gaaattttat aaacaaagtt cataatatcc aatacaatga
     2941 ctttttaaac acttttgaaa cagagagatt ttttgaaatg aaaaaacaca aagacgactt
     3001 tcatgaacag ctttcaaata aagtatatga agctttagaa aaagaaagtt tttttaataa
     3061 tgacaaatta gtatgcaata tcataataaa aatgtttttt tcatattcaa catatcttca
     3121 tatagaattt gaagatttaa aaccctatat tgaaaaatta atacattaag gtggtgttta
     3181 ccttgaatca taatactagt attaagaaca aggacagtaa tccaaaaata tttttagtac
     3241 ttacacttta tctattaggt atatttatgg gagctattga tactggaata gtatcaccag
     3301 caagaacgat tatacaaaat agccttggag taaatgaaaa aacgggaatt tggatgatta
     3361 ccatatacac attaacatac gcttctgtaa ttcctatctc tggaaaatta gcagataaaa
     3421 taggaagaaa atatgtgtat ctagttagta tatttctatt tggtagtggt tcattaatat
     3481 gtggattatc atcactattt tccaatttct atattttatt aattggtcgt gttattcaag
     3541 caattggtgg tggtggaatc atgccaattg ctacagcaga atttggaact accttcccag
     3601 aaaacaaaag aggcatggct ttaggtttag ttggggcaac atatggtatt gccaatattt
     3661 taggttcaag cattggtagc acaattctaa gtatctttgg aactcaaaat tggaagtggc
     3721 tattttttgt aaatcttcca atttgtttaa tcatacttat tggtggagtt ttctgtatta
     3781 aaaacaataa gtctgaatct aaagaaaaaa tagataagct tggtacattg atgttgatat
     3841 gtataatcgt atcactatta tatggtctta tgaatataga cttttttaat tttaaaaatt
     3901 ctatacaaga tatatcagtt tatccatact tattgacatt tattgtactt atacctattt
     3961 tcatatttat tgaaaataga gctaaagacc ctattttaag ctttgaatat tttttaaatc
     4021 cgagaatatt gatagtactt attttatctt taattgttgg tattggaatg atgggtatgg
     4081 tattcgttcc acagtatgct gaaaatgcat taaagataaa tgctggttct gggggatatt
     4141 ttgtagctat attgggatta tttgcaggta ttgctgctcc tttaagcgga agacttattg
     4201 acaagtttgg ggcaaaaaaa atactattac taggattttc tgtatctatg ataggctctc
     4261 tttacctagt tttaattgct ctaaaaacca atactgtatt tagtgtatgt gtaagtttaa
     4321 tatttatggg attaggaatg ggatttacta tgggaactcc tctaaactat atgatgttat
     4381 caaatacaag attagaagaa tctaattcag cacttgcaac attatccttg atacgctcaa
     4441 ttggtacttc aatttctcca gctataatga ttggatttat agcacatgca ggactatctg
     4501 tgcaagataa tattatggac ttagttggaa agccctcaac tcctaaaata gttcaacttg
     4561 aagaacttga aaacatgata gatgacttaa aatcagaccc tgaaatggca aaaaatttaa
     4621 aaaatgtctc tatcccaaat atgaatgata cttcaaatat aaatatggac atgacaggtg
     4681 tcaaacttcc aaaatcctta cttaataaag ttcagtcagc agatacaacc aacattacca
     4741 atataacaaa agaaatatca acaacaatgt ttgatgaaaa agttccatct ataattacta
     4801 aaatacagaa aaatgtacaa aaaggaatag atggaacaca aagtggaata aacggtattg
     4861 aagatggtga aaataaacta aatagtggta ttaaaggagt tcaaactggt attgacaata
     4921 tggtaaaagc tagagctggt attaatcaag gattagatgg tgtaaaaaaa ggtattgctg
     4981 gtgttgacaa aggtataaaa ggtatggaac aaggtcttga aaaacaagat gaggccatca
     5041 aggaacttac aactgcttat aataaaatac ctactaccga aaaaactcct actgataacg
     5101 aaaataactc aaatgaacaa aaccagagta ataccagtaa ttctgaatca gatgacacta
     5161 agaatgattc tacagaaaat aagaataaca attccagcga gaataaacca aatgtaaatg
     5221 aacaaaaaga aagtttaaat gctcaaatac agcaactaaa aaagacaaga gatgacttaa
     5281 atacaaagtt acaaaaaaat aaaactcaaa agaacgaatt aagtaaaaaa ctaagatcaa
     5341 tggaatctca aaagaaagag ctacaaagca agttaaataa ttctataagc caaaagaaag
     5401 acatggaaaa aagccttgat acaatgcaac agcaaaaaaa atcactccaa gcagttttag
     5461 caaagacaca agaggtaaaa actgaaatac caaaggcttt tgataaatca aaattggatt
     5521 atataaattc catagaaaat aatagaacaa aaatagaaaa cacactacaa tctactctaa
     5581 actcaggatt taaacagatg tatataacag tattctgtgt aaatctacta gcatttataa
     5641 tattattgtt ctataaagaa aataaaacta gataaaagag tgttatagta ttatattgta
     5701 aatttatctt attcttaaat attttattct aaagaataaa ttcataaaat ttttgtaatt
     5761 gattaatata aattacaata taatactatt tttctcttaa tctttttctc ttttacagac
     5821 aatcaaaaag caatcttagt ttatcgaaat aaatttatac aatctattca tacacccctt
     5881 tacattttta taacaattaa acctattgtt atatatacaa atcaaaataa accatatatt
     5941 ttcaactata atatgttaaa atcatattat gagcatatta atttaagcaa aatgtcattt
     6001 agactttaat ttagtagttt tatattataa atttatatat ttttaatttt aaatgtcatt
     6061 tagacattac taataaaaag aaaggagtgt attttgtgga tactttagga aaaagaattg
     6121 cttatttgag aaactccaaa aaacttactc agcgcaaact tatggatatt ttaaaatttg
     6181 aaaacttagg caagtatgaa acaggagata gaaaacctaa ttgtgatata ctaatgtcaa
     6241 tagctgatta ttttaatgtt acgactgact ggcttctcta tggtaaagag aaggtaaatg
     6301 tcaatagtag tgtaaaagaa gataaagaag attacttaca tgttactaat gatgaaatga
     6361 cgatattaaa tctatacaga caactaaacg aacgtgataa aataaaaata gaaggaatac
     6421 ttgaacttaa gatatctgaa tataaagact taaaaaagca ttcctctaat aataatgaag
     6481 ataaaatggt ttaaacatac ataatttgat acgattttac atcatctatt aaccttcttt
     6541 ttttgtagta tattactaaa atatggatat tggattattt aaaaacaagt atagtctaat
     6601 ttcaagcaca tttataatat ttagattatc ttatccatat tttagtttaa gtactttgaa
     6661 aaatataata catctctatg tataacttat attcctaaga aatgcattac tcatgttttt
     6721 aattcttcca acatctttct atacacaatt gtacatcctt cataattccc ctctttaaga
     6781 aggattgaac atgtactaga atatttagtt aaagtattta atctttccat atttaagctt
     6841 tttatattac tgagtgctgt aataaaatca tcaattgctc tacaacactc tataacccca
     6901 tctgaatcca atttacacac aagaatattc acctacttta tatactattt attaatcatc
     6961 ttctgaatat tctatattaa gtaattgata aattgatgat ttatattttt tacctgttct
     7021 tcttccattt aaaatagctg acaaatagtt ttcattaaca cctattttct tcgctaattc
     7081 cctttgagtc atatttaatt caataagtct ttgcttgact tgcactccca aaaaaggtat
     7141 ccattttttt cgagcatcca tttatcaact tcctttctaa gtgatttata tttactttta
     7201 tattatttta gcttacgcac cccctctatt tagtatatat ttttaatatg tacacatatt
     7261 aagtatataa atttatttaa tactaaataa gattttttga ttactttgtg ctaaaattag
     7321 gatgtaaatt cgtatcatta agttaattat aaagtcattt tgaccttaag tcaatgcttt
     7381 ttatgtcatt ttgaccttta atttgaaact atttacatct taaaaagtct ttttgacatt
     7441 aattgaatgg agagtgttta tggatacttt aggagaaaga attgtttatt tgagaaaagc
     7501 aaaaaacctt aaacaatatg aactagaaga aatgttgggc tgtgacaact taagtaaatt
     7561 tgagagaaac attagaaaac caaattatga aatattaaaa tctatagctg agatatttaa
     7621 tgtttcagta gactggctat taaatggaga taatctatca cataaatcag atttaatttg
     7681 cgattcctcc tcaaactatc cacttaatag cataaactct aatgaaataa agcttttaaa
     7741 caattttaga aagttaaatg attatgataa agctaaaata gaaggtatga ttgaattaaa
     7801 acttcatgaa tatgaaaaag agaaggattt aggaaaaatt gagtataaca aaaataaaga
     7861 tgagaaaata gataaataat ttttatgttg cttttgatac gattttacat catttctttg
     7921 ttctaatatt tttatttcat ttattttctt ttttataatt ttatgataaa ataattagta
     7981 tattttgctt tacttgaatg taattattta tacaaaagtc aaacataata taaatgtagt
     8041 aaatttttag tttcaagtaa tatctcgtta ttataactaa atatttatta aaattaggag
     8101 gataaaatgg gcttaagaga caaattcgcg caatcatttg ctagatcaaa aactatgagt
     8161 ggccctgaga aaaaggctaa tgaaataatg ggtaaattgc ttcttaagaa agctatatta
     8221 cctatcgttt taatgttcgt gataataatc gctggagcta tgcttaaaat caattcttgg
     8281 gttacacttg gcataaattt agttattgct gttggtgctt tcttctacat aagaaattct
     8341 agtaaaaagt atcagaactt taaaccttat gttggcaatt tgattagttt agagaaaaaa
     8401 ggtaaaaaag agtacgttgc tattataaaa caaggtaagt taccagttaa gttacaaata
     8461 gcttatggtg gtgaagacct tgaacatgtt aagaaaaatc aaatggttca aataagttat
     8521 aatccagatg ctaaaatagc tattttggtt aatagacaat aatttaataa aaaaaaggag
     8581 ttgtcttaaa attgattttt tctaatcaaa tttaagctca actccttttt taatttttat
     8641 atattatttt tatatattat ttttattact ttttttaatt aatattttta aatattaaat
     8701 ccttctcaat aaaatagtgc actatctaca atactacttt atcttttcct aacaatatat
     8761 aatctggtgc tactctttca tgttatacac cgttttgtaa catacctttt atatcagtaa
     8821 catcttcctt aatttcctct acatctgttt tcattattcc catttgaaca agtatatttt
     8881 tgtttatttc ttgttgttgt gtagataatt ctataaaatt ttctacagtt tttttataca
     8941 tgtctctatc ttcttttttc tcctgcatag tatttttaaa taatagagca catagtatac
     9001 ctattgcccc tagactactc aattcggtta ttaatttatc cattatagcc tcctattgtc
     9061 taaatttttt atacttatag ctttaatttt tgttgtattc ttacatcatg caatattaca
     9121 ttttcaggat tatcaattta atcttctgta ctttaaattg taggtgtaat ttatagattg
     9181 aataaataat aaatatatat acaatttcta atataacaaa aaacaagtac aaggcttact
     9241 tattaaagtt aagaccttgt actttacata aaaaaaataa ctaatacata gttattctaa
     9301 atttactatt caactggtat ttctaattct ttacatctaa ttctaacttg ctctctaatt
     9361 actgctggta cttcttctat tgtttttttg cctcttaaaa ttaaagctac atatacttct
     9421 accattccac tctcacctcc agattttctt acactaaaag tgcttatatt acttgcttca
     9481 ttgacagaac tttgatttaa caacatctca tatatttctg catttgctat aaaattattt
     9541 gttatatcat catcttgctt tacttgacct atacttatgt ctgagatatc tttatactgc
     9601 atttctactt ttataccttg tttgatttgt tctattttat ttttatcgaa tttaaacaat
     9661 tttcatacct ccattttaag cttgtggctc gttgttttca gcatcatatc taaaaatcat
     9721 agatggacgt tttatagctg tccatttatc tcctttttct actatgattt taggctgagc
     9781 actttttatg ttggcttctt ctaattcata gatttcttgg taacttgagt ctgatttttt
     9841 gatttcaact agtttcatgt ctttgaataa gtcagattta tttattttta cttgaacttt
     9901 agttttattt attaattgat atatagggtc tccaagattt aaatcttctt caagccctct
     9961 atctagtgta attgtaatat cttgttcatt ggatgttgta ttaactacct tgtacattct
    10021 attacctatc ataacataag aatctttaat tatagaattt ccagtactct ctttaaaaat
    10081 aaataaagat gcatataaat catcaccttt acaataagct ctaaatagta tcttattctc
    10141 tcctatttta attttatctg gtggtacttc aaatgatatt ttttcagtaa ttaaatcact
    10201 tacagatttt acactttcac catttataag catttctatt ctacttatat ctttattagg
    10261 gtcttgtatt tttatcttaa ttgatgaaat tgatttttca gtaatttcta tagcagcatt
    10321 tgtcgttcct acttctgata taatatataa tgtattgtct gttgcaagta catcctttac
    10381 tgtatttaca tcttcagaaa taggtattat agtaaattct tttaaaaatt ctactggttg
    10441 ttcaatttta tatacatccc tattttttcc agtaacccat actgaattat catttttaag
    10501 tactaatata tggtcacttc cataagattt tatttctctt atgtctgtta gtgcttttgt
    10561 aaagaactta attatagtat tttcactatc gcttcctaat cctaaagctc ccttatcatt
    10621 atatccttgt acaaacaatt cattacctgt agttactact aatgtatagt ttttaccaca
    10681 atgtacttct ttacaactag atatagttgt aattctttgt aattcagtaa ctggattatt
    10741 gtgacctata cctaattgat attcggaatt tttaccaata gaccaaagac tatcatcaga
    10801 ttttataaaa taagtaaagt tatctccaca tgaaacatgc tttacatctg atacattaat
    10861 ctgcacaaat tcatccactt gatttataga caagtcttgc aataattctc catggtagtt
    10921 tgtaccacat ccatacattg ttccatcatt aagtaatata ataatatgag tcttagaaat
    10981 agatacttct tttatatctt catatggaag ctcagtataa agatctcctt tgtataaatt
    11041 tcctaatact aaaccataat ctatgtcttt tgtggtaggt ataactatct tattgtttag
    11101 aagtataaag ttatgtctgt ttttaataga tgtcaaatct cccattacaa aattcatatg
    11161 cttataatta aatattctat tatcattatt tctactatcg tatctatttc caacacctaa
    11221 ttgtccatag ttatttaatc cacaaatata tatatcatta gtatctttta ttgctatatt
    11281 tgcatattct aaaggaagta ttttctttac tcttgtattg gtttttataa atgtatctct
    11341 ttttgttaca ttaccaacac ctaattgccc acaatcatta aaacctgtac catataggtc
    11401 tccattatca cttacataat gtccacatct ataattcatt ccaatgtcct ttgcctttga
    11461 tgctattttt ttaatcttct ctggagtctc acttcctgta cctgaaccat tactacattt
    11521 acctgcagta tatacatccc cttcacttgt aagtaaaagt actgcattat ttctgtttgg
    11581 agctactttt actacatttg aaattggaat ttcagaaaat gaactttgat tgctattact
    11641 atttagacct aagcaataat cagtatttaa acctgtacta tatgcagtat tatagattgt
    11701 tattatatag gtatttattt catttggtat aacttctttt acattttcca tacactttat
    11761 aaaactatac tggtcagtag tatttcccat acctaattgt gaattattat tgtatccaca
    11821 acaatatgca atgtttgtgt cctttacaat aaatgttgtt gtatctgagc agtacacatc
    11881 tcttacatta gttatattgt ttacagtaaa tgtcaatacg tctggatggt tgctttcgct
    11941 tcctaagccc agttgcccat gagtgttttc tccacatgaa tataatgtgt tatcatcttt
    12001 gattgcatgc gagtgagtag tccctaaaac aacatctctt acattttgga tgtttacttt
    12061 ggtaaatgta tttctattta ctatatcacc taatcccagt tgtcctttgg tatttaatcc
    12121 tgtggagtat agtgttccat catttctaat tgcaaatgtt gtgttaccat ctatacttgt
    12181 tgatattttc tttatattat caaggtttat ctgagtgaac ttatttctat ttattgtgtc
    12241 acctaatcct aattgtccag aactattatt tcctgtggca taggctgttc catcattttt
    12301 tactataaac attgtatttc ttgatgttgt aatttctttt atatcatcca taaatataaa
    12361 tgagtttgat tgttcagtta tatttgtgaa tttttttact ggttcgttgt tatattctcc
    12421 tatacctagt ttgttatttt gattcattcc tgttacatat atattattat ttttatctac
    12481 tactactaca gtatttcctt ttacaattat atcttttatg tcttttaatc ctgtatcata
    12541 attgcttgca ttacttatac tagttgaact cccaaactct ccattattag attttccata
    12601 tccttttaat acactttctc cagaataatt ttttagtata aactcatttc ctaatgatac
    12661 tgcaccatta cagttatcgc tcaaataact agaccaattt tgattagttt gtccatatgt
    12721 taaacaactt cctgtgcacc taaatgtaga attatttttt aataacgata aaatattttt
    12781 actatttcct acaacacttt ttgaactatc aaatactgaa caacgaatta gtttatatgt
    12841 ccactgatag caagtatatg ctactccatc tgaatatgta tgatatatgc ctgaattact
    12901 actaaatctt aattcagatt ctggagttat ttgagaagaa cttgtaaatt tcatattatt
    12961 ttcaccaata caataagctc ggtcactttc atcaaaaaac atacatccat gataaaaagc
    13021 aaataaattt attaatttag tttgagctgt attataaaat tccaatgttc tatatgaagt
    13081 attccaagca ttatatcctt tactctgata tgatgaacta gtactaggtg ctggatatcc
    13141 tccactacca ttacatgaat atcctatagt ctttgcaacc ccagttgaat ctagtatata
    13201 taaagtatca tgccacgtag ctattttttc tacattgcta actctagtat ctttgtaagg
    13261 gtagtttcta ttatcaccat aaactatata agattgattc ccccacccat ataattcttt
    13321 tgaatgattt aatgctataa cataatttcc ttcaacacaa aattccttaa tattttctat
    13381 ctcaattttt gtaaatatat ttctatttgt attgtccttt aatcccaata taccagaact
    13441 atttaaacca caagaatata aagttccatt attcttaaga acaaaagtcg agcttccatt
    13501 tccttttata tccaaaacat tatctatatc caccttagtc ataactgacc tgttaacagt
    13561 gtctcccaat cctaactgcc cagcattatt tagtccacag gcatataatg acccatcatt
    13621 cataacaata aacatattat tatctgttaa ctctatcttc ttataatcct gaaaattatc
    13681 acttaacttt atatttaaaa attcactatt atcctttgta acatataaat aatcatcatt
    13741 aaccaataaa cctgaataat tatctaaatt tgaagattgt acctttgaaa actcatacag
    13801 aaaagttgta tggaaaaatg aaccaagttg acctgctaaa ttacagccag atacaaatat
    13861 ttcttttgaa gcatttatta aaaaattaaa ttgagaacca catttaatat cttttatatt
    13921 ttctacatta accttagcaa aagtatttct tgatgctaca tctttattgg cattggctag
    13981 ttgaccataa ttgttttgtc ctgtactaaa tagtgtatta tcgtatttta atatcatagt
    14041 atgattattt ccacaagaag catacttaac attatttata ctagaaagag taaatacaat
    14101 tgaattatta ttattccctg taccaagctg accaactcca ttatagcctg tagtatacat
    14161 agttccatca ttcttaataa gaattgtatg actactacca cacgctatct gttttacatt
    14221 atccatacct tctattttat taaatacact tttatttaaa gtactattaa ttcccaactg
    14281 tccacttgaa tttaatccac atgcatgtgc tgtcccatcc atttttatga tgaatacaga
    14341 ttgattataa gtcactacat cttttacact atctatattt actttagtga aagtagctct
    14401 attgttagta tcacctagtc caagttgacc acttgagttt aaacctgctg aatataatgt
    14461 tccatcattt ttaagtacaa aagtaaagtc ataaccacat gcaactgttt ttacatcatc
    14521 tattggtagc ttagtaaatt cataatatac tgtagactca attacaccta attgacctac
    14581 attacttgtt cctgctccat atgcaaatcc atctttagtt actgctacac tgtgttttga
    14641 accacaagat acatatttta cattatcaat ttttacttta ctaaacatcc ttctctctga
    14701 cgaatctttt ccaagtccta gttgaaaacc tgtattatcg ccacaagcat ataaattacc
    14761 atctgatttt aaaccaaagg tactattact tataccagta aaaaattcta caaaatcata
    14821 atcattatat cttttatcaa aatcatcaat caatatgttc ttatcattaa aataagcacc
    14881 acgctgtaaa agtttatttt gcttcattat tagtactacc tcctttattt atcttatttc
    14941 atatgaatat ttacttagag ttatatcttt ttgttctttt aaataagtgt tactagattt
    15001 tgtctcaaca aagttattat ccatatcatg tatatatcta actctatttg cactaaatgg
    15061 taatactaca tctctaaaaa tcacattacc aatttcacct gttatacatt ttctatttaa
    15121 atctaactta agcccttcta tagctactat gttatttcca tttgctaatg tatcatacca
    15181 ataaccagcc tcatctacag ttacttctga ctctaattct aggtatatta atatatctag
    15241 cactttttca gataatttgt tatagctatt ttttaatact gatatatcag tttgtatttg
    15301 accacttata acatcatcaa aagatatatt ttctattttt ttaactacct catttatagc
    15361 tcctgataaa tctttagaat ctgtagttaa tatatctgta tctccaattt ttacttttat
    15421 ttcttttata tttttatcaa acttatcatc taaatcttga atatcctttt gagttacaaa
    15481 aaccaacgtc gggtctactt ctaatgtaac atttgaagta ttggacaatt gtaaaatcat
    15541 ttttacaacc aaatccttaa tactgccatc ttcagcacgt ggcttatacg tctctggata
    15601 cttacctata gctattatat tattctcatt atccagaaca gcagcttctc ttatcataaa
    15661 tccaccaact gttcctggta aaatagtctc tataactatc caattaggat ttttttcatc
    15721 aattcttaga gaatttatat ttccttccca aactacattc tttaaagcag tttgttcctc
    15781 tgttggctca taatattctc cattactatc tccaacttga aacttggtta atccaacaag
    15841 ctctcctagt gcagttgcat ttgctatttt ttctctacca attttggtta atattatata
    15901 aaatttttct tccaattata ccacctccga tttaggataa actactacat tttctacgcc
    15961 tgtatcattt gcaatagcta tattaatctt acaattattt tcaatgcatt taatgcttaa
    16021 aggataaacc tttatttctt ctctacttaa cattccagct ccacagttta cagcagcact
    16081 acattttata attggttcaa agtatggatg aacttttacc tcttcactac ataccatacc
    16141 tccaccacaa tagaccatac ttttatctag tattattggt tctaaatagt gtaataagtg
    16201 agcaggtttt actctttcaa tcatctcgct acaatctaaa agagttttat agtcacagtt
    16261 atttgctata aaactcaata cgaatgtaaa ttcatcacta taaactttta tatctgtttc
    16321 tgattttgta tatgcctcac atatactttt tataacttca atagtactag tacctctgct
    16381 tctcatttta gcttttatat tgctacgtct tgtgtcaaaa tcaagttctt ttttttcaat
    16441 gcagagtatg tcttcccaca tatctaatcc ccatgtcgct gtatcaacaa ataattggtt
    16501 aaaggtatca tcaatatctt ctttaagaat atttaattct gtatcgtatg caccttgtat
    16561 ctcctctaca atgtaatttc tatcaaatga tggtagttta tcaattagct tcattgattt
    16621 tccacctcac taaaattaac agttgttaca ctaggtattt tatcttcatt aatagttata
    16681 ttatctgtac ttccatttat aagtaggtta cttaaatcgt gtacaccagt agtatttata
    16741 agtattccca ttacttttgt atagattatc tctcctctaa tatctctaaa gtatgtattt
    16801 atactttcta ggaaagattc ttttacatta tctaatgtat atccatcttc tagtttcatt
    16861 actgcactta tacttatttc tattggtaat ggtgtcacaa ctgttatagt tggtccaata
    16921 ggcttctctt catctatatg ttgctgacat ctttcaatcg tttctgtatc aacagcttga
    16981 ttatttttcc caaatatcaa gaccttaact gttcccggac catcccatct tggataaacc
    17041 ttaacattat agactccatc tacttccaaa gcccattctt catagtgagc tttatttcca
    17101 cttgtagctt gatttctttg gattttataa aatctttctt ttagttcttc atctgtctct
    17161 atatctgtac caccttcaaa acttcttgtg ttagtaatct ttgttactcc agatatatta
    17221 tcaactagtt taaattcaca atttgtaggt aaattatatt tcttaccaac ttccagagct
    17281 tgaactgggc tattgtcacc ttcttcacta ccaatagtta catcttttat tactacaaat
    17341 agtaaatctc tatatgatat tattgtgcca ttatttatta cagtcccttt ctctccaata
    17401 aattcaactt ctccatttga ctctgtacct aactttctat atacaccaaa ttcattgact
    17461 cttttatcaa gaaactggtt atatgtgtct tgtataaaag ccattttatg tatctttgaa
    17521 agttctagat atatcttcga aagttctaga ttatttccag ataccatgtt gtttagaaaa
    17581 gaaccttctc ctttataaat atcaagatta atattttcaa gagttctatt ttttattact
    17641 tcatatgtct ggtcactata catttatttc cacctcccca tagatagtgg atacttttac
    17701 atttgcactc aatacgtctc tgttaaagtt agcacttttt acgtttactt ctaatatata
    17761 tggatttatt agaagggcct cttttatgaa tctactagct tcactttctg taagaccttt
    17821 tgtatatttt tgtcctatta gttctgaaag ctctgtccca tattctaaag agtatatctc
    17881 atgttcatat ctatttgtct ttatacactt gtatatccaa acttttaagg cttctttttt
    17941 ttcaattatt ttaaagtcac ctttttctaa aataggttca tctttttcaa aatcccatgc
    18001 cacttcacga aagattggca attcttctgt tttaggtaag atataatcct ctgggacacc
    18061 tataaaagga aatattgtac tcattataag ctcaccaact tacttacaac agcaaatttt
    18121 tcgcctattc taaacattat taccatatcc ccagattcaa agttatctat aaaaggattt
    18181 tttacttcat gctggtgttc ttgatttgtt tctgtgtcaa atgtttctag ctgtctatca
    18241 atcatccaac tatctatcaa aatatcttct ttttctaata tgatgttatt tgtctctatt
    18301 tttaaatctg gtaatttact tttaattttt ccaataaaaa aagaaggttg attatgatac
    18361 tttccttctt ctcttattat tcctataaat tcatttattg gattagccat tatatcacca
    18421 tccttttata atactctcct agctgtatta aaatctcttc tactacttaa cttacttatt
    18481 ttaactacat cacctttttg tggagcatgt aaaaattctc cattacctat atataatcca
    18541 acatggctga ctggattatg aaaaaatact aaatctcctg cttgaagatt tttttgttct
    18601 actttcttgc cttttttaga ttgttgattt gacgttcttg gcaaactaac attaactttt
    18661 ttaaaacagt agaccattaa accagaacag tcaaaactac ttggtccatt tccaccccat
    18721 ttataaggtt tacctagatg tttttttgct tctgatatta ctattttagc tttatcagat
    18781 acattaccag aataaccacc aataataatt tttccttttc ttcttccaaa tttactagct
    18841 tcttcaacat ttccaaataa aatatctata tggtatgttc catttttttc tatagttatt
    18901 gcaggtccta catcatttac tttatagact ccatcacgac cagaaactcc tgtaattacc
    18961 tgtatttcat ctccatagtt caataaagga tgtttattta aaaactcttt tgtataataa
    19021 gtttgctcat atttaccaac cataggagca gcgcaagttt ttttagatgg gtcaagtttt
    19081 ttctttctac aatctgtatc tccaccttct tcttttctag gacagtaagc tgtaaattct
    19141 gctgtaaact cttttcctcc tgcataatct tctcccccta aattactttc ttccttaggt
    19201 tcatcctgtc ctgctgactt ttcatccata agattttgaa aattaagttc aagctcaatt
    19261 tgatattctc cattttgcca agtatgtttg tctgtatcta tataaaatag tcctacaagc
    19321 tttgtataag aatctttaac ttttactcct ctaccagtta tacaacttac atctccataa
    19381 cctttaagag aacagctttt ttctatccca ttaaactcgc tatcaatatc tacatcttga
    19441 ttttcttgtt gctgaattac tttttgcatt attacattta cttccttaaa aatttcatta
    19501 tctatttttt cgctaatctt gcttccatac tggtctacca ctattacttt attttttaca
    19561 ttttccatgc tctccgaaaa ggtggtattt ataatattaa atccctcttc aaacataaca
    19621 cttaatataa cagttccttt ttcaataaca ttaaacttat ctaaattagc ctctatcata
    19681 tactttttct ttgtcttttt acttgcctct gtatatgcac tcattattgt gtcataacca
    19741 tttactccta taaacatctt tgtatacttg acattggtct ttgctattgt tccaactgaa
    19801 agcctatttt cagcaaatac ttgctttgct atgtcttcaa ctaacttatc tttaaaattg
    19861 tatgacactt cactttgtgt aagtaaaaat cccatatctt tagatacaaa actaatttca
    19921 ttactgcttg aatctttaga cctattaatt atcattcctc taaagagttc tttctcatct
    19981 acatagaaac agactgtact agctattggt atatcgattt gttggaaatt tacatcaaat
    20041 gatgattgta ttattgaaaa ctctagtgtc cttgatggag atttataatc acctgaccat
    20101 gataccttgt ctactatgtc agttatgtca tatatacttc cgttttttat gtgtacccaa
    20161 atcttaataa taaaaaatca cctgcctttt catttaattc tatcagcagt tacttacaac
    20221 ttattatcta taatagttat tgttttggag ttacttttgg tatctttatt cttttatact
    20281 cttttaaatc taatgtaaaa tatacatctc gagtcccatc tctttcttca taactaaacc
    20341 cttcaattat gacttccata tttatatttg tttccgtaat tgtaaatctt aatataaaac
    20401 cttccttcat ccatttttct atcttattta cacaatcata tggtgatgga aaacctgtat
    20461 agtcacagaa agtgtagtct ttatttggaa aaaaacttga tattgaaatg cttttaagag
    20521 ctacaccacc aaaagttgct acttcaccta ttttgagtat actagaagag tttacagcag
    20581 cttttccatt aatactaaag gaagatggaa atactggaaa tctaaatcta tcttctgctt
    20641 gtctaagcca catttccatt ataagacacc ccctaactgt gccaattgga tacttgcaac
    20701 taattttgat gttatctttt ctatatcagc ttcttctcta atcactattg tatcagctaa
    20761 cttagctatg tttatatttc caccattttt tctgcttcta tattgatttg cttcttgttt
    20821 agttaaaacc atttcacctt catggagtct ggtttgatag tcattataag gaacatagtt
    20881 caatccaaat gcattattac ctttcttact tgaataagct ttaccagaat caccagcctt
    20941 tgttccagca tatggattat ctttgtaatt ttttttactc ttttccacta gatttaaaac
    21001 tgttcctgtg atgcttccat tttttatctt attaatcata gtttgccacc atttgcatat
    21061 atctttaact attttacata ctttatctaa tatgttaaaa aaagctccca atattggctc
    21121 tataattttc catccagttt ccaacagagg tcctaaggtc tcccatacag ttttccaaat
    21181 aacacccagt gtctctataa aattgtttat ttgaggtgca tattcaccta aaaaagtaaa
    21241 aattcttgtg ataataggac taacagcttc aactacaggt ccgattagtt ggaatgctgt
    21301 agttactatt gtaccaaata tacttattaa tccttgtgca attggtgatt tattgtttat
    21361 actatctagt aaattattta ctaagtttac tactggaagt aatgaatcta ttaatgctgt
    21421 aaaatcaata ttttgtaata gtgtacttcc tgcgttaaca attgcttttg gtgcattccc
    21481 tatcattgtt gctgcatgat tttcaaaacg ttcttgctta ttttcaacca tcattttacg
    21541 cttttcagcc tcattagaaa ttcgatattg agtagaatca gaaaactctt ttccattttt
    21601 tacacttcta gattgcatct ggtcatattt gttgccaata attatcttac cattcattac
    21661 attgcctatt ttaccagcca ttttttcaga taagccagct aaattcccta atccactttc
    21721 tataaaagtt tttattttct ttagtccttg acctaaaata tcatcaaaag gtttaacaat
    21781 actcttcaaa ccattgttaa tgcttgtttg agctcctgat attatacctg gtattgtatt
    21841 actcatttct ttagctaacc ccatctgctt tacacttatg ccttccaatg cttcatcaaa
    21901 agttttataa ttttctccta ccagagtatt aagagattcc atatttcctt ttgctgcact
    21961 taagaaagct gctgatacat cttgttcact acttgtattc atatttaaag ctctaacatc
    22021 taccatagct tgagttatat ctttttcttt tttgccacca gtaatattac taatcatttt
    22081 agctttagtt cccattgcaa caacatcttc aggggcaaaa ggcgtatcat ttgccatatt
    22141 tactaaactg tcataatact tatcaacatc tttttggcta gtttttccag tatttctttt
    22201 cattacactt tctagaacaa ctttctgatt ttgttcctga ccaagtaagt caacaccctt
    22261 tttcacagcg acacctatag tagcaccatt tgtcaaatca gatatcttcc cttttatcga
    22321 accaagtatt ccacttgcca tatcctttac agctattgca ccactccata ccttacctgc
    22381 aaacaatctc aatttgggaa atactttact tataatgcta cttgccatat cttttatagc
    22441 tattacacca ctccaaaaag tatttttaaa caacattaac ttaggcaaaa ttctagccat
    22501 agcactactt accatatctt taagattaat acttccttga taaggacgtt tctctactcg
    22561 ttttaatttt cccaagatac tacttatcac tctcttagtc atatcttttg cttttattac
    22621 gatattccaa atcttgccac caatcttttt taaatttcca agtaatttat ttttagtttt
    22681 ttctacttca tcacttttag ctttaaatat taatataatc ataatatttg aattaaactc
    22741 atttttcttt tgaatcaact tattaaaata atcaataatc atagctatag ttattgctag
    22801 tctcataata cttccattta aacttccaat aactcctgaa acagcattta tgctagaact
    22861 taattttgcc atagttgaac ttacactact agcagtagtt tgaacttttc ttaacgagga
    22921 atttaaactg tttactctac tacttgcact tccaatagaa ttttttatgc tactagccat
    22981 tacattagtg gaaagtgaaa cagccacagt tgctctccta aaattatcag ttactgatat
    23041 aatttggttc atttttggtg tgtaattatc tgtaagatac atatttatac cttcttctct
    23101 cacatttccc attacatacc acctcctgtc aaaggacaca ctccatatcc atgtttcatt
    23161 ttttcaactt cttcctcaat ttctaaggcg aaaaaagctt caataagctt taattcgcct
    23221 ttatccattg cataaaaaag ggacggtctt ataccttttt tcttccaata atagtacatt
    23281 attgtagtct tggtatccgt ccctattagt tttttacctc ttctatcact gcatttttac
    23341 cataacccat aacatcttga agtattctat ataggttggt aatttcacct ggtaataata
    23401 gttttcttgc caattctttt ggtgttggag ccttaaattt atccattagt tccttatttt
    23461 taaataaaag ctttccatcc aaatcacaaa caccttctat aattgtaagc atctgcatct
    23521 ccggtaagtc tatatccgtt ttcatatttt catcaatttt aagacaagta ttttgtatct
    23581 cttcatcttt acttattgtt aaagctctac acattacttt aaattcctgc ccaaatatag
    23641 ttgataatct cttaagttca actatagtac ttggtctttc taagttttct gtatctgcat
    23701 ttaaaagtaa atctactatg tttatatttt ttgataatcc attttcattc attattattt
    23761 gctcctttat caatcaaatt caatcaacta acttttttac ttattaacta cctaaattta
    23821 ttaagtatat atctactaaa tatcagtata aaaccaaata taaaactaaa ttatatcaag
    23881 aaaatcatac tcagtaaatg tgaaaggtgc ttctatctct ccttttactc caacctccca
    23941 atcagctaaa gttaaatcat caaatgctat attttttact gctattcttt ctgctccata
    24001 agaatctggg tcatttaatt tagatatagc aacaaatcta ggttcagaac ctctctttat
    24061 cttttctcca attaacttgt gcattcttga actaacatgg tatagagtta ttgaaccttt
    24121 tcctttatat cccatatact ttgtatcagt acccatttga cctgctatta taatatcctc
    24181 ttttgtaaat tccatctttg cttgaaactt ctttacttct gctactttgt ttccatcaag
    24241 ccaaagttct ccccaagtac cactcattac atttctagct tccatatttg ccatattaat
    24301 aatcctccta tatttctatt gataaatcta tatcttccat agcatcaagt acttttattt
    24361 ttgcttttaa aaatacttta gaacctgtgt tagcttcttt tatttcttgt aatgtcatat
    24421 aagataaatc tactcctttt gattttaaat acgatttttg tgcttcaaaa tctatttcaa
    24481 cagtagaatc agattctata agtgctgact tttctaattc ttctaaataa ctttttatag
    24541 ctactatcaa taaacatttg ttgtcataac tgttagtaac ctttcctata tagtcatcta
    24601 ttatcacctt tcttatgtca ctatgtataa tatctaaagt gtcaactatt tttattttct
    24661 ggaacatttc tcctttttct tctgttaact cagttaaaga atttactcct cttgcaattc
    24721 ttatagcccc tgcttcctta ataagtataa gctctccttt attaaccctt gattctgcat
    24781 caactttcgt catcttaggt atatcaacta catcactaag cttagtataa gttactgatt
    24841 gacttaaagg tgttcctgct ataagtccag ccaccctact tgtaaactca tcaacactgt
    24901 atttcttttc tccagctaac acatcttctg tagtaaaatt aattatccct tcatgatttc
    24961 ctacaacttt tcctaataca gctttaacct taaccttatc tatatctcta agtttaatta
    25021 tccaattttt tatagcagtc ttatcagctt ctactgcttt tggcatacat agataattaa
    25081 attccttagt ctctaaaaaa tctaatgcag tttgaatatc tgcttctcct tctattacat
    25141 aaactaataa tttatttgga gtgttaacat ttcccatcaa agctaaattt atatattctt
    25201 tattttcagc agataaatca actggtatat cctctttttc atgtatttca ttaagaccta
    25261 gtgccttagc atctttaagc accattgcaa ttattcctct agctgaacgt tctttaacag
    25321 ttgtagctag ctccttaaat gatatgttga tacttggtaa tcctatagcc atactatcac
    25381 tctcctaaat ttaaattgat ttcttccata ttttcatata ctacatcttc ttcatataca
    25441 ctatccagat aatttagtgt aattgtaaag ttcaatacat aaattgactc ttctttttta
    25501 atactttgtt ctagctttgt tatatttaat tttctgtctc ttacctttat atttctgttg
    25561 aataacttat ttaattcatc tgaaatttca tacattctaa tactctttga ctgtggtttt
    25621 ggtaagtatt ttatagagat aacaatatct ttattattcg tatttaacat tataggtgta
    25681 caaataactg atagtatact tacaaaaaaa caagaccctt cagagccttg cacactttca
    25741 tcatcaataa aaatactttc agtaaagttt tttttgagaa tctcaataat tgtttctaat
    25801 atttccttgt atttaagcaa ataatcacca cactatcact aaatattctt gcttaatctg
    25861 ataatcttac tataaatgat ataagtcatt tttaaatata catctcattt attcctgtca
    25921 aatatcttct atatttttag gtaatattat tcttttcaat ttatcacctc cttcaatatg
    25981 gatttctttc acaataacat tatctcacat attttattgc ctagtgttgc aatagtgtat
    26041 ataaagtgtt gtaaagttgt catatttttt ataatatctc actcaattta aatatcattt
    26101 ttttctttaa attgtagcaa gatgttctat ctaagtgtag tttcatagaa atatatgtca
    26161 tattgttttt tgtcttacta ttataaaaaa gattaaaaaa ttccatctct atatcattta
    26221 gacatgttag agcattttct atcttctctt tttctatttc tatatcttct ttttccattt
    26281 ttaattttct taatctttcc tctcttttta acacctcatt ttccacagaa gaacttatgt
    26341 tatatgtgtt actagtcttt tctgtatata ccattgctcc acatccagag tattcattct
    26401 cacatctttt tatgtccaaa ttaatattct ttatctttat atccaaatac ttataattat
    26461 gtaatctttc atctgtttct ttaaataata tatctttttt gttttccata gtacttctcc
    26521 ttttttatat acttattatt agcttcaaat ttacaataat ctctttagtg cacagttttt
    26581 tactaatctc acagccttca agaacatatt gttctataac tgtataaaaa aaacttcttc
    26641 tatattttgt tctaaaatac tagaaatctt cttagccata agaagtccag gattttttat
    26701 acctctttct atttgagata tataatcatt tgtaattcca gcctttctgg caagttgaat
    26761 ttgtgtcata cctttgcatt ttctataata tttcaagtta tttttaaaca ttgttttata
    26821 cctccattac cattaagaat attttgttct tccacactta gattatatag aatcatttgt
    26881 tctttatcaa tagattttag aaaaaaatat tcttttatat tttattagtt ttatttttta
    26941 gaacaatttg ttataatcta tacataaggt ggtgttataa tgtttgccaa aagattaaga
    27001 gaacttagaa aggaatttgg attgactcaa agagaacttg gagaaaaagt aggcgtttcc
    27061 caaagagtct tagggtacta tgaaacagaa aacagatttc ctgatgaaca cattttaaac
    27121 aaattggctg atgtatttaa tgtatcagtt gattaccttc ttggaagaac cttagttaag
    27181 gaaaatattg atacagtagc tgcacataga aaaaatccac atgaagagtt acctgaagaa
    27241 gcacaagaac aacttaatga ttatatagaa tttttactaa ataaatataa aaaaaaataa
    27301 accatatgat gagcagtcta tttttctgct cttttaatat aatttaaagg caaacataca
    27361 ttctatttta gggggatttt tatgaataat ttggataagc tttttgaatt agcttctcaa
    27421 gaagagataa taattcatta taccacttat attgcaggtg atttagaagg gctatatata
    27481 aacaaacatg gtattaaaat tatatcatta ctcagtaatt taaaacaaaa ctcaaaaaaa
    27541 ttgacatcca tcttagctga agaacttgga catcatttca ctagccttgg atactatgta
    27601 tcctcttata acgattatta tacaaaaatt attatagata agtgcgaaaa taaagcctta
    27661 aaatgggctt gtgagttctt aattactgaa gaagatataa taaacataat taattctggc
    27721 attacttgcg tttatgaaat ggcagacata cttaatgtgg atattacttt ctttcaaaaa
    27781 agattagaat ttttatcatt aaaaaaacag tctttacaac ttggaaataa taaatattta
    27841 atattaacta atttgccgta tttctacata tttgatccaa tttcataata aaaatagtag
    27901 taattttaac tggcttaaca gtaaaatcta tatatttatt ttaacaaatt ttggtttcta
    27961 taaattcaat attaatatat aataatgata tcatattatt taaaattcta aaaacaaata
    28021 tatgcttttg ttaagaaagg ttgtttgatg aatgtataaa gaaaagttgt ttgataatta
    28081 ttttaagttc ttagcactat tattttggcc aattatgtgg tataagtgga tagtaatttc
    28141 aaatggtact cttgaaaata tgttatttac catatatgca atcgtcgcaa ttgttttcat
    28201 cattttatat tcggtgttta tgataaaata taaagacatt acacaaatag atttctttta
    28261 caggataagt actctacttg cttttatatt tacacttttt agctttttaa tatacccaaa
    28321 gagtttattt ttcttgtatt taaagattat ttttactgga atataccttt attattcaat
    28381 agtaaaaact cttaaattta aagatgacga aggtgtagtg ggtattatga gctcactttt
    28441 attaattgta ataactttgt tctactaaat ttaattctat ttgacttaat atggctttaa
    28501 atattagaca tctacattat tttctgatat tatttaagat ggtagtctta tattaataat
    28561 gaaatacact atgaaggagt taactattat ggaaaaaaaa gttttagcaa cagttggaga
    28621 aaaagagata actaatattg atattgaaaa tgcattaaaa tcattagacc cttatcaagc
    28681 tatgcaattt aaaactgaag aaggaaaaaa acacttatta aatgatttag tgaatcaaga
    28741 attattcttc ttagatgcta aagaagaaaa attagatgaa gaagaaatat ttaaattaga
    28801 aatgaaaaag atagaagaaa atgtattaaa gcaatttgct ataaataaag ttttatctag
    28861 tgttaatgta acagaagatg aaaaagtaaa attctttgaa gctaataaga gctctttctc
    28921 taaaccagaa agtgcaactg ctaaacatat attagtagat agtgatgaaa aagctaaaga
    28981 aatacttgct caaataaaat cagaagaaat atcttttgaa gatgcagctc ttaaacattc
    29041 ttcttgccca tctaaagata tgggtggtga tttaggaaca ttcggtagag gacaaatggt
    29101 tcctgaattt gaagaagctg tgttctctat ggctaaaggt gaagtaagtg aaccagttaa
    29161 aactcaattt ggataccata taataaaatt agaagacctt caagaaagta cagagtctac
    29221 atttgatgaa gttaaggctg aagttgaaaa aagtttatta tatcaaaaac aaaatgaagt
    29281 ttatggaaat aagataaatg ctcttaatgc taagtacgga aaccttgtaa aatacaacga
    29341 ttaatcataa ggtcatatat aaaaaagctg tcttttcaca atatttatta gaaaagacag
    29401 ctttttatat taaagtttaa atattcagtt tcatattatc attcttttat tgtaaagaac
    29461 tcagcttaaa agtttaaata agatttttaa ttatcattag ctaaattttt atatccacgt
    29521 attatctcat aacagtaacc tattacatct cttcttttta ttatcccaat aaatgtgtct
    29581 tggtcatcta taacaggaat gaaattttga tttaaagatt ttataactaa atcttcaata
    29641 tctgcgttga tggaaacagg agaattatcc atttttctat ttatatctgt cactggaaca
    29701 tcttccaatc cctttaagtc cagatttaaa tcatttttta atgtccataa aaaatcacct
    29761 tctgttatag ttccaacata tttaccatcc ttacttatta ttggaattgc agagtatttg
    29821 tgatattcca ttttttctag ggcttgcctt atagtgtagt cttcatatat atatgctact
    29881 tcactttttg gtgttagaaa gaataatatg ttcattacac tacccaccgt tactgaaaac
    29941 ttataaatat tactcaatat ttataatgtt acttttttgc agtaactttc tcctttctta
    30001 ttttctaagt attcttaata ctatacgctg taacaagcgt ttttcctttg ctcattttaa
    30061 tcttattatg ctttattaat atttttatat ggtctatcag tatcacttat tctcttttta
    30121 gaaaatttac taaaaattaa ttgacttaaa cttagccata ataattttac tctacactat
    30181 taattttaac atatttatct cattattaat gattttattt taataatttt aatttatttt
    30241 gctattaatt ttatttacaa aaactgaaaa aaccgctaaa tgattagcgg cttatctctc
    30301 atattactag aacactaata tatatgaatt tttaattata atctaactac gttagcagct
    30361 tgaggacctt tagctccatc tactacttcg aactcaacgc tttgtccttc ttctaatgtt
    30421 ttgtatccat ctccagttat agctgagaaa tgtacgaaaa catctccttc accttctaca
    30481 gatatgaatc caaatccttt ttcgttgtta aaccatttaa ctacaccagt tttcattaat
    30541 aaatcctcct aaaaaaacac aaatatttag ctactctttt ctgtaacctt attactactc
    30601 tatcatatat agctatgttt gtcaattatt attattttta tacattaata atcaattttt
    30661 ttactttata attttaaatg attattatct tttacacttt atttttcttt ataatttttt
    30721 aataatttat ttttattata tggaatctta tggacttatc actccaacct aaatcccatg
    30781 gcaccaacat tctatcagta ttatgacaag ttaccaaagg ataccctctt gagtctaatc
    30841 ctgtcactgt cgaaacatgc gttattctgc ctttcttctc ataaccaaca aaatcccctg
    30901 gacgtaaatt ataagcttct ttgtatactt cttcatagga acctttagca attaatgaac
    30961 ctcttccact atacagtgca taatttttaa aagcttgtgc atttacccat gcttttgtac
    31021 ctgctctctc gctataattc caaattgagt tctttttgaa ccttccactt tcaaacatta
    31081 tctgtgatgc aaagtttgca caatctccac cttctggatt ataatcccta tagtttggat
    31141 taaatttcat accatgttct tcatcagctg cggctccaca gtatttatgt gcataatcta
    31201 tagccttttt ttgttcatct gtaagctgta actctacacc atcttgttgc ccaatataat
    31261 ttcttatatc ctcagatttt atattttcta aatttaaaga gtctgcaaaa gggtctgtat
    31321 accactcttt agttatgaca tattgattat ctataatttt tacatttaga taatgatatg
    31381 tacctattct aaaggcattt tctttgtcct tttgattttc atatgaatac ttatattcag
    31441 tcgatactga acagattata ccatagaggt ctttttcttt ttcttttatt tttttaatct
    31501 taacccttgt ctttatgtct ttaaatttta caccttgctt actagaccag ttttctaaat
    31561 atttcatctt ttttacttca ttttcataag cccatagacc aaatttttta tccgtctcat
    31621 aaagaccttt taaaatgttt tcatttttct ctaatattgc cttatttctg tagtcaaata
    31681 agttttcaag caatagttta tattggtctt ttacatcatc accttcaaca tttgcagatt
    31741 ccatgacttt ttcttccata tttttcatac ttaaatcatt gattaaaaat acacctgctg
    31801 tagtagctaa agacaaaaat acaaatgcca ttataccttt gatgtactta ttcttatttt
    31861 tcagtatttt caaagctaca cctcccaaat agtacacctt acaaatcaaa ttacaaagaa
    31921 tattctgtca catatagtaa ttttcatatt taataattct ctattttatt atgtccaata
    31981 ttaaaaatta attgtatcaa tacactgttt tttttacaat tttttatttt ataatctact
    32041 tttttctttt ttttatagta atatatatgt atataaatat tttggtatca ttataattaa
    32101 tacatactaa tggagggttt tttatgcaaa aaaaagtcgc agcaattaat gacttatctg
    32161 gaataggaaa atgctcatta tcggttgcta tacctatact ttcagcatta aaggttcaat
    32221 gttgtccatt tccaacggcc atactttcaa gtcaaactgg ttatcctgaa tttactttct
    32281 tagactttac cgataaaatg gtaaaatatt caaatgtttg gaaaaattta aaagtcaatt
    32341 ttgatagtat atatagtggt tttttgggtt caaagcatca aattgagata gtggctaatt
    32401 ttataaacga ttatcccaat gcttttatcg tagttgaccc tgtgatgggt gataatggag
    32461 ttatgtatcc aatattcaca gaagaaatga gacaggaaat taaagaatta gttaaacatt
    32521 cagatttaac tactcctaat ttaacagaag cttgtttctt aactggaaat gattatacca
    32581 aaagcgatta taatagagat gagcttatat atattgcaaa atctgtatct gatttgggac
    32641 ctagcaaagt cgttattact ggtatattag aagatgataa tatattaaat ttggcttacg
    32701 atagagataa tgaccatgta ttctttacat cagtcaaata taataattgt tcttatagtg
    32761 gaactggaga tatattcaca tctatacttt gtggtatgtt agtaaataaa catgatttgg
    32821 gagtagcggt taatactgca acagatttta tatataaaac tataaattac acatctcaat
    32881 ttgatactga tagaaatgat ggagttatgt ttgaaaactt tttatcagat ttaactaata
    32941 tataaagtca aaaaaactat ccattttata aatacctata aatcataaaa tttaggtatt
    33001 tacaaaatgg atattattta ctaaactatt ggtatttcaa ttgttaccat agcaccacca
    33061 gttttcacag aattttcaat atataaaatc ccattatgtt tttgtacaat attatttaca
    33121 atatagagtc ccattccata atgagattta gaatttctac tcaaatctcc tgtataaaac
    33181 tgttctgtag cttttgacaa atctgcttta gaaaaaccac ttccacaatc tgtaactaca
    33241 aattttaagt atttattacg tactgataca cttatgtata gtttactctg gcttggtgaa
    33301 taatctatag catttgaaat tatattcata atagctctga ataacagttc tctatctata
    33361 gttatatttt taggcaaatt atcttgttta taaaacttca catctaagtt tttagtacta
    33421 gctaatgctt ctatttgcac taagacatct tttataaact cactcatact gattgcttct
    33481 ttatttaata tatagccatt ctctgttttt gatatatcta ttaaagtatt aatatatttt
    33541 tctatttgag tgacaccctt tgagatatat tccatgtatt ctaataactc aggattttca
    33601 tttatttcaa ttaataagtc agtatttcca tgtataattg ttagtggtgt ttttatatca
    33661 tgtgccagtg cagatatctg ttcctttcta ttttcttcta gcatccattg tttttctaaa
    33721 gaatctttta acgccaactt cattttatcc atagaaaaaa ggacattatt aatttcataa
    33781 attttactag attctacaac aaaatctaaa tcctgttgtt ctattttttc tgtagtattt
    33841 tttaaaagct ccatttcaat attaaatttc ttgccaaata cttttgataa tataacaatt
    33901 tcaataaaaa atattatact aaaaataatc atacccaatg tctctggttt tggtaaatat
    33961 gctcttaaaa ttggagaact atactctgat actattttgt attttattat aaaaacttca
    34021 tctttcttaa aaaactttat ataactttct gaatatgcat gcctttcttc aatatctctc
    34081 attaaattcc atacttcttt tgattctttt ttattaaagg tacctgaaat taaattccca
    34141 tctaaagtat aaaccccata ctcatatata cttggaatta tatcttcagt tactttttca
    34201 cttgaaataa tctgttcttt atatttattg aattgttttt ctgcataatt agctggtaaa
    34261 attacacctg atgataataa tacagaaaat gataacatta aaaggagtac aattgaaatt
    34321 gtcataataa agaataaaga taaatatttt ataaaaatag ttcttaactt ttcaactttt
    34381 ttgttgttag attttttacc tccaagcatt tgtaccctgt cctccaatag tttaattaat
    34441 tcataattaa tacctagcca gttttttatt tgattatttt atatcttttt attgctaagt
    34501 tatattttaa cataataata taaataaaca gtcaattttt actctattct tctgattttt
    34561 taccttccca tttagaaaac caaatacata aaattacaaa tgctaaaatt gttacaaaac
    34621 cacaaattaa acctgttgat tgatatgctt ttattgattc aacctttata tattcaaccg
    34681 ttttagaact tgaaaaagac gaccaaatag ttataaatct aacaccccat gcacaaggaa
    34741 aatactgcca aataccagaa cccaatccag ttaataataa tgctgaaagt aacatttcaa
    34801 ctatacctac tccaattgat gctccttttc cgaaccttaa acttaagaat aaatgtaata
    34861 tgtattcaaa taaattacac ccaattaaaa ttagaattga aatcatataa aagcttgatg
    34921 taaagtaagc cttagatata ccaaatttaa aacctaatac agacactaat attgctacaa
    34981 aaccaagaca tactaagatt atatatttac ttataagagt aagatatttt ggattagatg
    35041 ttgttaacat atgtttataa ttccctgcat aatattcttg ttcaacaaac attgaacaca
    35101 caacacctga caataaagga aaagctattg ctataacttg taaatatgct actatcttta
    35161 gtatattatc agctcctgaa accttataat accataaaaa caatcccatc cccattaaag
    35221 caatgcaaat gtgcattagt attataaaat ttctctttaa ttttattaaa tcagaacaaa
    35281 atattcttat taactgtctc atattacttc acctctctat tattgaacca aacccttgta
    35341 gctaaagcaa ataatacaaa taaaacaata gatattaata ctcctggcaa aataacagca
    35401 ttatttaaaa attcacttcc ctctggtaca ggaagaccat ttggcagaat atgcaataca
    35461 acagccatta atcttgaagg aatcgaatat ggaaatatat accacatttc tgtagttgag
    35521 aaaacagcaa ctccaacaat agtaaagaac atatttgtta gaactgtaac aaatatacca
    35581 aattttgaag ctaaaaacat acaaagagga atctgccaca taaatagtaa aataattaat
    35641 atagttgcaa tgatattttg acctattgaa atagtttctt tatagattat cccaacaaaa
    35701 actacaccta ttgcagaaat aattgatgct ataaaacaac aaataataca gtaaatcatt
    35761 tttccagccc aaacgtcttt catttcaatt ggcaatggta aaataccacg atatttactt
    35821 ttttcatctt ttaatactat taaagaagat attaatgtta atgctcctgg aagaaatgtc
    35881 gtataccacc agttgaaagc agcattttgc cctccaccta gtccacacaa aagtatacaa
    35941 aacactggaa ctatccatat caacttcatt ccaaaagttc ctttcatctt ttgaaattcc
    36001 actctaataa atctttccat attactgccc tctctttcta ttttcagcta caactttcat
    36061 aaaaagtttt tctaagtttt gttcttttga tacttctcct tgataaccca ttactccatt
    36121 agaaattatt cctatttcat ctacaacttg ttccacttca gacaagatat ggcttgataa
    36181 aataactgta attccttgtt ttggaaaaga acgaatcaat tctctaagtt cttcaatacc
    36241 aatagggtca agaccatttg taggctcatc taagattaaa agttttgggt gatttaataa
    36301 tgcaatagca ataccaaggc gttgcttcat gcctagagag aattgacctg aacgcttttt
    36361 aaaggtatct cttaaatcta cagtctctaa aacttcttca atgcgagagt caggaagacc
    36421 aagtaaagta gtatggactt ttaaattttc tcgtgctgtt aaattctcat atatagcagg
    36481 agactcaata agagaaccaa tatttgataa atcctttcta gtccatctat gtccctcaaa
    36541 cattattttt cctgaagttg gatttaaaat cccagttatc atttttaata aagtagattt
    36601 cccagctcca tttggtccta ataatccata tatagaatta gatttaattg ctagtgaaat
    36661 gttatcaact gccatctgct ccttaaattt tttagaaact ccttttacct gcaaaatata
    36721 attactcata attttcatcc tttcttttgt caaacttatt tacaagaatc attctaaatc
    36781 ataaatataa ggattttata agaattaaaa aacaaaaaaa gatgtgttaa aaacacatct
    36841 ttttttatct taattctatt aattttccat tgtagtatat ctctacttct tcaatccaaa
    36901 tgctattatc ttcttcttgg atgacatttt taataatatt atttatagta tatattatat
    36961 tttgttcatt caaataatca tctttccaag aaactctact ttttttatct ggttctatca
    37021 agtttatttt aacaagatta tctttttcct taacttctac ttccatgtct aaaccattaa
    37081 aatactcatt tgataatgta tctaagatta atcgcagttt atccttaagt gaactttctt
    37141 catcaatctg taagtacttt actattttct catcctttga aattatctct ataggtattt
    37201 tagtagtttt tttagaatta atcattttac tagttgtttc ttcttttgca tcttcatcct
    37261 tctttacaac tgctgattct ttagaatcaa ctagctcaac tgcactgatg ctataatcca
    37321 tacatgccgt actcaataaa gtagtagcaa ttattaaaaa acttactgct attttattca
    37381 taagtatccc tctcctaatt ctctttcgat acttattata ccatagtata gattatattt
    37441 ttatactttt aaatcaattt ataaattatt atagtatatt atttacaatt ctaagctatt
    37501 tgaatttatt tttatagtat attctttctc taaattcgac agatttttat tcaaaagccc
    37561 ttttatttcc ctcgtatttc tatacttgtt gctatgttct tcattatgtt cataatcata
    37621 tgaatcattg tcatattctt catcattatt aaattcttca attgcttctt ttgactttat
    37681 atcacaattg aatttatact tatttactgg tatatcaagt cttaccttag aatttggaac
    37741 ttctatgtcc aggaacttag caattggcat atcagagtac aagtaaaatt cactttcata
    37801 atttgaattt ctacctattt tctcttttat atttacattt tcaggtatat actcttctat
    37861 attattgata aatacatctc cattctctga agatatatcc aatgactctg attctatgtt
    37921 tacattttta acgcctaaaa ttgattttgt tgacaagtga agattgttat tagatattaa
    37981 gtttaggttt tctaatctat ttactttgtt ctctgttata gctctactaa aacctccata
    38041 agaagtttta tatgttatag tatttgaagc tacattatcg tatacaaata agtgtgagct
    38101 tatacttgat gcatttatgt ctacattatt tggtacatat attataattt ctctataatc
    38161 atgtgaaaac atactattta tagatgtatt taataattgg tcgaaatttt taatcttttt
    38221 aattttattt tcatattttc tttcaccttt tacaactaaa gtcttatctt ttaaagttac
    38281 attgtatttg taaaactcat tatttcctct tgttgttaca cgcacatctt cacttgaact
    38341 tttctttatt gtaacgttta tattgtcaac agctatatct aacttgtcaa tatttacttt
    38401 ttcttgataa agtttatttt cttttttaaa ttcagcttcc ttttctaaac caaagtttat
    38461 taggcttggc ataactaaaa tccctgacca tgttgtccct attataccta ttaccaataa
    38521 aactgcagca aatatagcaa gttttttatt catgtttatc ttctcctttc ttattttcta
    38581 tatcttcaag gtccttatat atcgcctcaa aatttatact atcatcatta tattcatcag
    38641 tttcttttat gctttcttct tctctattgt agtaatcatt attatatgaa tcttcattaa
    38701 tgtctttatt caaatcttca tcatttaaag tagctttttt tcttttttcc atcttttcat
    38761 ttgcctttat ataaatattt tttctcttta tccaatgtat ataattattt agtaattgtt
    38821 tacctagttt tatggcaaat aataaaattt gccatgcaag tatctcaagc cctacaaatg
    38881 ctatggttgc gaaaatacaa aacatcataa catctggaac cattgctata aatttaatca
    38941 caaatggaat taatataaca aaagcaatta atgagcatac taatgcaata gtagcagata
    39001 caatagcaca tacaaacgca aatgctggaa caattagaaa taaagataac actactaata
    39061 acccctttat aacttgtctg gataatcctc ctacaccttc ttctatactt ggtgtaagtt
    39121 tatcatttat attttctttt gatttattgt acttttcctt agcttcatct ttcaagatag
    39181 agtaatcctt ttttacattt gatttaaatc tactaaattt accatttaat atgtctgtat
    39241 tcattatttt tccatctata ttctcttttg atttactata cttttctttt gcttcatctt
    39301 ttaagataga gtaacctttt ttcatatctg atttaaattt actaaactta tcattcaaaa
    39361 catctgtatt tattattttt tcctcttcaa tatttttttc tttagtctgt aagataagtt
    39421 cttttgctat agattttggt gagccaagtg atgctattat ctccatatca ctctttccct
    39481 ctataagacc atctacgaaa tactcttcat aatctctcaa tatatcatta acttcgctct
    39541 cagaaaaatc tttttttaga taatccttta aaatttcaag aaactcagtt ttattcaagt
    39601 tcattacctc cctcatcttc acttattaaa atctcaacag cctttacaaa ttctctccac
    39661 tcttccatca gattggataa attctctttt cctagtgaag ttattctata gtattttcta
    39721 gcaggacctt cattagattc tagtatatat gtttcgaaat acaaatcttt tgtaagtctt
    39781 cttagaatag gatatattgt tccttcattt acatctatta ctttagagat gttatgtact
    39841 atctcatatc catacatatc tttcttagat atgagagcaa gtacacaaat ttctaaaaca
    39901 ccctttctaa attgtgtgtt catatactcc atcctttctt taaatacaca gtattgtgtt
    39961 ttacaaggta ctatgttaaa acttcttata tcctcataat aacacagtac tatgttctgc
    40021 acaatagtta tttttaaata ttcttgttga ttaaatcctt ctataaatgt ataatactct
    40081 aatcagatat attttatgaa agtggtgata aattggctaa aatgaaagat gataaaacaa
    40141 tattaatcct aactgctcaa tttggtgctg gacatataag tgctgcaaaa gcagtcaaag
    40201 aatgtattat tgaaaaatat agtaactata atgttgttat acaaaacttt ataaatgcaa
    40261 gtattcccat gatgaataag cctatggtaa aactttatga aaacaataca aaatatactc
    40321 ctggattata taattactat tattacttta agaaatcgtt tgattcaaga catgatttat
    40381 ctcataaatt atatacacct aaactttctg agtatattgc tgatataaat ccagatttga
    40441 ttatctctac atttccacta gctgctgctt gtgtaaataa ctttaagata aaaaatcctg
    40501 atataaatat acctacactt acagttataa cagatgttgt agatagtatg gaatgggttt
    40561 ttgaaaatac agatttgtat tttgttcctt ctcctgaaat aaaaaataga ttttttcaga
    40621 aaggaataaa tccagactcc ataaaggtta ctggagttcc tgttgataag agatttcaaa
    40681 ttgaaagtaa agaactatgt tgtgataaat atagactatt acttctaggt ggaggaagag
    40741 gtctatttga tatagatgag gattttatgc attggataga tgaatttatt gaagaacata
    40801 gtaattctat agaaattaca atagttactg gtaaaaacaa aaaattatat gacaatctaa
    40861 ctcataaaaa acctctaaaa aatattaaag tacttggatt tgtaaacgac atgtataatc
    40921 taataagaga atgtgaccta atgctaacta agcctggagg agcaactata tttgaagcta
    40981 ttcaatcaca aactccagta ctcgtaaaaa tgccaaaagt tggacaagaa attgaaaacg
    41041 ctaaatttat aatagataag ggtcttggca tgatttatag tgatgaccta gaccttaaaa
    41101 acatatttta taaattagta tcaaatgaat ttgattctat aataaacttt atgaaaaaaa
    41161 atttaaaaga atttaaaaca gtcattcatc cagagaaaat tgctgattat atatcagaat
    41221 taataaataa tcattacagt taaattgtac tattaaaaaa tttcaaacat aaaattaaag
    41281 gcaatcaagt atgcttttac acttgagaat ttactcatag tgtttaataa tacttgattg
    41341 cctttatcat atcaaaatca aattgttata ttagtatata tttttcttgt actgcatata
    41401 cagttgttgt atttcttcca tacttctctt taattctatt tgtaaatctg aagcagtctg
    41461 gtaatcctta ttttctatag cttctctcac tctagtgaat agtgattctc tagttaaagt
    41521 atccttcata atattgaact ttaaatccga tattgagttc caaactacct catcaagcgc
    41581 atccatattt tcttttgtgt gtatcttgac aatacttctt attttttgaa tcccagcctc
    41641 tattattcta gtttttaatt tcttttgcca cttatctata gctccaatct taaatgattc
    41701 tattatacta tcagtaaata catcaccttg ttttaaagat tttaattttg aagcatatat
    41761 ttctaagttt ttcatatttt cataaactgt agctggtggt attccaaatc ttgcatttct
    41821 ttcttccaaa ctataatgtt caaatacatc attttcatct ctatatgctc tatctttttc
    41881 taaatagaaa ctttcttcac ctacattttt agataactct ttttcaagtt cttttgtact
    41941 aagaccactc ttagctgctg cttttatacc atccaacatt gtttgataac atcctgctat
    42001 tactaaatac gtgttagata gagggcttgg tgaccttaat tcaaatctta cagtctttgg
    42061 atttttcata tctcttatta atccaacaag cactgaccta tttcttgatg gaatctcata
    42121 actatgccct aaagatgtaa ctatacatac aggagcttca aatccaggaa ctaatctatt
    42181 aaatccatca ttagaagctg ttacaattgg gtttaatact tcataattgt aaagaagtcc
    42241 cataagagcc ccatatccta gttcacttag ataatcttct tttaaatctt ttggagcaaa
    42301 tagatttttt atacttccat cctttaactt cgcacttact cctacatgtg tatgtccacc
    42361 acttcctgct actccatgaa ttggttttgc tttaaatgta acttctagtc catgtgatgt
    42421 aaatacatcc tctataacat ctcttactaa taattcattg tctgctgctt gtaatggtgt
    42481 agaaaacttc caagaaacct ctaattgctc catagcatgg tttgttcttc catctatact
    42541 aatagaactt tgtattcctc cgacctcttt gtgagccatt tcagggttta cacctagctt
    42601 ttgaagtata attaaacttt tctctaaaca agttcttata ataccatgag ttcttttcca
    42661 gtattgctct tttaggcttt gtgatacata taatttctct aagtcagctt tatcctcagg
    42721 agtattaacc cagaactcaa gttctgtagc tgctgttaac attatttcct ctatatcatc
    42781 tactgaatct attcctatgt tatttattac atgtggatat tctctaaata tttcatacat
    42841 tgacttttta aagtacttat ctgctttttg aagtactcct ctagaacata cttttttatt
    42901 atcatgtatt aaaaatgcag gtatctttaa tgttccaaca ggtagaccta cttcttcgtc
    42961 tatatattcc atattgtaat ctacatacca atgacaactt ttatctggca ttaaatctac
    43021 tttcgcatta tttaatgtag ctatattata aagctcaaca cttgaaccat cagtctgtat
    43081 agctgattcc aaaaatttat ctatatcctc caaaaataat tctacaggaa tcttttcatc
    43141 tgttgcattt cctcctaaat caacccccat taaggaaaca aatcttatat ttttattgct
    43201 ttttaggatt tctcttaatt cttcattagt gtgtttatct ttctctatta cgtataatag
    43261 actatccatt tttccactct cccataattt ttatgtatat tttattttgt taaatacaag
    43321 ataaatttca tttttcttac atttatgaat ttttcttgca aattattcgt gttttattc
//
LOCUS       DAEMHZ010000016        42720 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000016 DAEMHZ010000000
VERSION     DAEMHZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.016"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..280
                     /locus_tag="KPK72_000776"
     CDS             <1..280
                     /locus_tag="KPK72_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG2488020.1"
                     /translation="KTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRNDYWIFNPD
                     LDPVINILPEEFFFHSGMMILILILLVSILLFVMYKLCKSLSIKIFQC"
     gene            complement(422..682)
                     /locus_tag="KPK72_000777"
     CDS             complement(422..682)
                     /locus_tag="KPK72_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG2488021.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            899..2182
                     /gene="hflX"
                     /locus_tag="KPK72_000778"
     CDS             899..2182
                     /gene="hflX"
                     /locus_tag="KPK72_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2488022.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVNAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            2190..2867
                     /locus_tag="KPK72_000779"
     CDS             2190..2867
                     /locus_tag="KPK72_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG2488023.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2953..3396
                     /locus_tag="KPK72_000780"
     CDS             2953..3396
                     /locus_tag="KPK72_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2488024.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3701..4345
                     /locus_tag="KPK72_000781"
     CDS             3701..4345
                     /locus_tag="KPK72_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG2488025.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4431..4808)
                     /locus_tag="KPK72_000782"
     CDS             complement(4431..4808)
                     /locus_tag="KPK72_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2488026.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4808..5194)
                     /locus_tag="KPK72_000783"
     CDS             complement(4808..5194)
                     /locus_tag="KPK72_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2488027.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5373..6128
                     /gene="nadE"
                     /locus_tag="KPK72_000784"
     CDS             5373..6128
                     /gene="nadE"
                     /locus_tag="KPK72_000784"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG2488028.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            6211..6930
                     /locus_tag="KPK72_000785"
     CDS             6211..6930
                     /locus_tag="KPK72_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2488029.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGEGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(7097..8101)
                     /locus_tag="KPK72_000786"
     CDS             complement(7097..8101)
                     /locus_tag="KPK72_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2488030.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8218..9114
                     /locus_tag="KPK72_000787"
     CDS             8218..9114
                     /locus_tag="KPK72_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG2488031.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9252..10514
                     /locus_tag="KPK72_000788"
     CDS             9252..10514
                     /locus_tag="KPK72_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488032.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKKII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10590..11783
                     /locus_tag="KPK72_000789"
     CDS             10590..11783
                     /locus_tag="KPK72_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2488033.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12412..13206
                     /locus_tag="KPK72_000790"
     CDS             12412..13206
                     /locus_tag="KPK72_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2488034.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13301..14641
                     /locus_tag="KPK72_000791"
     CDS             13301..14641
                     /locus_tag="KPK72_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2488035.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKKENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14778..16010
                     /locus_tag="KPK72_000792"
     CDS             14778..16010
                     /locus_tag="KPK72_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2488036.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            16050..17192
                     /locus_tag="KPK72_000793"
     CDS             16050..17192
                     /locus_tag="KPK72_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2488037.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17203..17997
                     /locus_tag="KPK72_000794"
     CDS             17203..17997
                     /locus_tag="KPK72_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2488038.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            18008..19030
                     /locus_tag="KPK72_000795"
     CDS             18008..19030
                     /locus_tag="KPK72_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2488039.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19129..20505
                     /locus_tag="KPK72_000796"
     CDS             19129..20505
                     /locus_tag="KPK72_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2488040.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20836..21180)
                     /locus_tag="KPK72_000797"
     CDS             complement(20836..21180)
                     /locus_tag="KPK72_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488041.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21668..22678
                     /locus_tag="KPK72_000798"
     CDS             21668..22678
                     /locus_tag="KPK72_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2488042.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22814..23302
                     /locus_tag="KPK72_000799"
     CDS             22814..23302
                     /locus_tag="KPK72_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2488043.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23478..25406
                     /locus_tag="KPK72_000800"
     CDS             23478..25406
                     /locus_tag="KPK72_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2488044.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMSIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            25583..25993
                     /locus_tag="KPK72_000801"
     CDS             25583..25993
                     /locus_tag="KPK72_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG2488045.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            26015..26317
                     /locus_tag="KPK72_000802"
     CDS             26015..26317
                     /locus_tag="KPK72_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG2488046.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26534..27226)
                     /locus_tag="KPK72_000803"
     CDS             complement(26534..27226)
                     /locus_tag="KPK72_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2488047.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(27252..28439)
                     /locus_tag="KPK72_000804"
     CDS             complement(27252..28439)
                     /locus_tag="KPK72_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2488048.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWTPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28775..30652
                     /locus_tag="KPK72_000805"
     CDS             28775..30652
                     /locus_tag="KPK72_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2488049.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIIPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            30964..32370
                     /locus_tag="KPK72_000806"
     CDS             30964..32370
                     /locus_tag="KPK72_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2488050.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRVQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAINYYRTNCAEALPEDSQHTFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            32398..33246
                     /locus_tag="KPK72_000807"
     CDS             32398..33246
                     /locus_tag="KPK72_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2488051.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKTFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            33907..34599
                     /locus_tag="KPK72_000808"
     CDS             33907..34599
                     /locus_tag="KPK72_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488052.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            34596..35531
                     /locus_tag="KPK72_000809"
     CDS             34596..35531
                     /locus_tag="KPK72_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488053.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            35679..36602
                     /locus_tag="KPK72_000810"
     CDS             35679..36602
                     /locus_tag="KPK72_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488054.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            36586..37356
                     /locus_tag="KPK72_000811"
     CDS             36586..37356
                     /locus_tag="KPK72_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488055.1"
                     /translation="MKELLDIISCEFSKLKWLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            37366..38148
                     /locus_tag="KPK72_000812"
     CDS             37366..38148
                     /locus_tag="KPK72_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488056.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            38525..39061
                     /locus_tag="KPK72_000813"
     CDS             38525..39061
                     /locus_tag="KPK72_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2488057.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            39093..39482
                     /locus_tag="KPK72_000814"
     CDS             39093..39482
                     /locus_tag="KPK72_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2488058.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            39519..39905
                     /locus_tag="KPK72_000815"
     CDS             39519..39905
                     /locus_tag="KPK72_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG2488059.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            40032..41474
                     /locus_tag="KPK72_000816"
     CDS             40032..41474
                     /locus_tag="KPK72_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2488060.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            41771..42586
                     /locus_tag="KPK72_000817"
     CDS             41771..42586
                     /locus_tag="KPK72_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2488061.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        15916 a         5009 c         7996 g        13799 t
ORIGIN      
        1 gaaaactaca tcaataacac ttataattat gccattatta ttaacagttc caattttatt
       61 aaattttgaa aagtcattta ttatttttca taaattatta tttagaaatg attattggat
      121 atttaatccc gatttagacc cagttataaa tatattgcca gaggaatttt tttttcactc
      181 tggaatgatg attttaatat taattttgtt agtaagtata ttactgtttg taatgtataa
      241 attatgtaaa tctttatcaa taaaaatatt tcaatgttaa atagagggat atttttattt
      301 ccaaaataag ataataaaaa aggattggat taaaagttat acaataaaaa tagctgtgca
      361 ataatattta gactatgaaa gtagttgaaa aatatttatg cacagctatt tataatagaa
      421 tttacttttt actagtgatt ttaactaagt cttgaaattt actcataatg taattgtaat
      481 tatctttttt atgaggtaat atacaactac tgcaatcttt gataccttta tctgtatact
      541 taaagtttcc accacagttt tctcctaaag catacaaagg acaatagcaa aatagacagt
      601 taaactcttc tggtttatta gttttatgac aagggaaaaa ttcgcaatct ttgtgattaa
      661 aaaacttata attttcgccc atattttcct cctaattaaa taaaatttaa attttttata
      721 ctatataaga ttttggtgat aatataatta tactaaataa ttttatgaat agtcgataat
      781 atagtattat taatcatagt attaatggta taattagtca acagaaaaat aatgtaaaaa
      841 taatgtaagt gtattttgta attagaatat gttttataac aaaaaatgag gtgattaagt
      901 gaaagaaatt caagaaaaag cacttcttgt aggtctgaat ttgactacaa tagtgaaaaa
      961 aaatgatgat atagacacta atgaatctat ggaagaatta aaagaactaa caaaagctgc
     1021 tggggcagaa gttgtaggta gcttaattca aaataaacac tctgtaaatg cagcttatta
     1081 tataggaaaa ggtaaagtag aagaaataag agcctatagt gactctttag atgctacttt
     1141 agtaattttt aatgatgaat tatctggtgc tcaaatcaga aatatagaga atgttgttgg
     1201 aagaaaagta atagatagaa ctactttaat tttagatata tttgctcaaa gggcacttag
     1261 caaagaaggt aaattacaag ttgaattagc acaattaaaa tatagattgc cacgacttta
     1321 tggaatgggt ggtgagatga gtagaacagg tgcagggatt ggtactagag gtcctggaga
     1381 acaaaaacta gaaatagata agagacatat acttaataaa gcagcagata taagacgtga
     1441 acttaaagaa gtaaaaaaga atagagagac acagagagtt aagcgtttaa agtcaaatat
     1501 accgattgta gcacttgttg ggtatacaaa tgcaggaaaa tctaccctgt tgaacgaatt
     1561 aataaagaca cacaaggatt atgaacaaga aaaagaagtt tttgtaaaag atatgctttt
     1621 tgcaacttta gatgtaactt tgagaaaggc tcttttgccg aataaaaaag aatttttagt
     1681 agtagatact gttggatttg taagtaaact tccccatgat ttagtagaag cgtttaaggc
     1741 aacattggaa gaagtacagt atgcagattt aatcttacat gtaattgatg caacaaatac
     1801 tagttatgaa ttgcaaaaaa gtacgactga aggtgtatta aaagaacttg gtgtaaatga
     1861 taaaaaacat atacttgtat ataataaagt ggacaagcta gaattggata tatatcctaa
     1921 aagtcaagaa gatattgtgt atatttctgc aaaacaaggc ataaatatgg ataagttatt
     1981 aaatatgata gaaattgctt taatggaaaa tacttattca gtaagtttga tgttacctta
     2041 tgagagaggt gatatattta gtagaataaa agataaatat aatgtggaga attttgagta
     2101 tggggaaagt ggtataacgc tagatgtaga tttagatgaa gaagatttta atatctatag
     2161 agagtacata ctagaaaaat aggttgaata tggatataaa ctgggaaaat atagataatc
     2221 ttgaagatta ttttataacc tatcttcttt ataaagaatc taaaacagta tctcaaatta
     2281 gtaaaattag aaatatttca agtactgaag caagtgagca acttatacaa gcaaagttaa
     2341 aaataaagga aatgcaaaaa gatgattttg aggcatctaa ggatattttg gataaatttc
     2401 tagaactaga taaaattaag agacttgatt ttatggatag cttagatgac gaaaaaatgg
     2461 tatactttaa gagaaaggtt ttcaaaagaa tccttgtaga aaaaaatgct gaagatttaa
     2521 ttgttttaat ttgggcgaca ggtgagctaa aagatgatag gtttttgaaa ttgcttcatc
     2581 agctcacaaa ccacagacac tcagatatta gaagaataac ttattcagca atcagaaaga
     2641 ttgaatcacc aagtagtaga gaagtactgc aaaaaggtct ctacgacaaa aatgcacaga
     2701 ctaggcagta ctgtgcaaaa gctctttcaa aattgggaga tgaaaacagt ttaaagattt
     2761 tacagcagtt gaaagacaag aataagaatt ttgaaaaaga atatgtgctt agagcttatg
     2821 acgaagccat taagtcattg gaaaatgcaa aaaatttgaa aaagtaattt tttattaaag
     2881 caatttgttt acttaatgat gtatttctaa agtttaacaa ggtaataaat aaattaatgg
     2941 gaggggtttc aaatgatagt aagacgttgt gcaggtggag tagtgtttta tgctaataaa
     3001 gtacttattg taaaaaatga taggggtgaa tggacacttc caaaaggaaa gatactagga
     3061 gggggacttc catatgagag tgctgttcaa agagttaaag tagaaactgg catagatgct
     3121 aaaatgatag atgtggctgg cgatactatg tatgaatttt tctcaagaag tagacaacaa
     3181 gaagtttgta atgcaataat gtggtatgtt atggaagctt gtaacactga ttgtgtactt
     3241 gctcctgaat ttcaagaagg tggattttat aaagtaaagg atgctttaga aatgttatca
     3301 catcataaag agcaagcttt agtagaagta tcttataaaa agtttaagga attaaagaaa
     3361 ttagtttcag agggcgatga tgttccagca aattaattgt gaatttagag gattctgagg
     3421 aagggatagc cccttccttt tttaattgaa gcagttttaa gcgtaaatta tatagtctct
     3481 ttaaactatg atttatcaat ttagagattt agtagtttat atttaaaatc agtaagatat
     3541 atttaaaata aaaattaaaa agtatacaat tttattatat aattgtctta tgatgttgat
     3601 taaagaatta ttaaaagtcc atttattttt aaatagaata attttataaa tatatatttg
     3661 atgtgtatat tagaaaatga ttaaaatagg aggaaataca atgagcaatt acaggacatt
     3721 acatgaattt ggaacggatg aaataacgat tgaaaagtct gtttttattg gatatgctaa
     3781 gcctataaag agtgaagaag aagcattaga gtttataaat gaaataaaga aaaagcataa
     3841 agatgcaact cacaatgtgt gggcttatac tgtaggtaaa aatatgaata tacaaagata
     3901 tagtgatgat ggagaaccac aaggaacagc tgggatacct acattagagg ttataaaaaa
     3961 agaagatttg agagatgtag cggttgttgt tactaggtat tttggaggaa caaaattagg
     4021 tgcaggtggt ctagttagag cttatacaaa aggtgctaaa ctgggacttg aagcaggtaa
     4081 gataatttat aaggtaatgt atcaagaagt taaggttaag atagattata ctcaacttgg
     4141 aaaagtgcaa aatgagttaa tgaatttggg gtattttata aaagatactg tatatgagga
     4201 taatgttgaa atagtagtat attcaagatt agaagatgta gagaaattat cagaaaaaat
     4261 gattgatata acaagtggaa caggaaaaat agttttagga gaggaatttt atttgtctga
     4321 acaaaatgga gaaattttaa tataatctaa aaggttatta gaatagttaa tacctcaaat
     4381 agccaattag tatttttaag tgaaaagcca ttaaattgat ttaatctaaa tcaatttaat
     4441 ggctttttat cggattttgc acagaatcct ttttttactt caaataaacc gactaaaagt
     4501 aaaaatatcc caaatatttt ttttaatata tctgaagaca gataattagc taatagtgaa
     4561 ccaaatacag ctcctaaaac cccaaacaca gctataggta taaccaattt aaaaattaca
     4621 tttttatttt ttatatgtat agcaagtgct ataagtgtca tagggataga tgaaagtaaa
     4681 tttacgcttt gtgctatttt agtgtctatg ccagcaagta aaacgagtgc aggtataagg
     4741 attgttcctc cacccattcc cattcctcca ataattcctg caaaaaatcc aattagagca
     4801 aataacatta aaacaccatc cttaatgctg caattatcat aattattcca aaagaaattc
     4861 taagaaactt tccagtaacc ttatttagta atttagctcc tataatacca ccaagaatac
     4921 ttccaactgc aactttaatt gttaagttaa catcataagt acctttagat acatataaaa
     4981 cagaacttgc tgtcgttaag aatattatta tagaaagagc agttgcatga gatttatgtt
     5041 cttctacctt aacgatgtca tttaatattg gaactaaaag agtacctcct ccagaaccaa
     5101 aaactccatt tataaatcct gtaaaaactc caattatcgt attttttaag ttaaaatcaa
     5161 agtatttttt ctttaaatca tcagtttttt tcataaaaat caccttctga aatagtttta
     5221 ccaaaatagg caaattatat ttatataaaa taagattaaa tataatcttt catgtttgtt
     5281 taattttgca taaaaaaaaa catattaaaa caacatatta atatatggta taatatatta
     5341 aaacaaagta gcaaatttga gaggagataa caatgtcaaa tataaaaata caaatagaca
     5401 aaactgtaga atggcttata aataaggtta atgaggcaaa tgcaaaagga ttaattgttg
     5461 gtgtatctgg aggaattgat tcggcagtag ttgctaattt aattaaaaag gcatttccag
     5521 aaaactctat gggagttata atgagcataa aaagtaatcc tcaagataga gaagatgcac
     5581 ttaaggttat agagggatgt gatatagaat accttgattt agaccttata gaaccacaaa
     5641 gtgctatatt agacatggta gtaggaaatt taaaagataa acatttatat agagaagaat
     5701 atttaaagat gacagatgca aacttaagag caagagtaag aatgagtaca atttatacaa
     5761 ttgctaacaa tctaggatat ctggttgttg gtacagacaa tgctgcagaa atacacacag
     5821 gatattttac taaatttggt gatggtggag ttgatatttt acctattgca aatttaacta
     5881 aaggagaggt gtatgaatgg gcaaaagagc ttggtgttca tgaagattta ataaacaaag
     5941 ctccttcagc aggtctttgg gaaggtcaaa cagatgaaga tgaaatggga actacttaca
     6001 atatgataga tgctgtgtta gaaggtagat tggatgaagt tcctaaaaga gaccaagaga
     6061 taattgaaag acttcataga ttaagtgaac acaagagaaa aacgcctgca caacctccta
     6121 agttctaatt tactatttat aagatttgtg ataaaattta caagtgatga aaattagaat
     6181 aattaattgt acgtattaag gagtggaaat atgggacgta taggtaatat aattaataga
     6241 aaaggtaaac aagatgctca aagagcaaag atatttacta aacatgctag agcaatagca
     6301 gttgcagcta aagaaggtgg agcagaccca gagtacaatg cagcgttaaa aacagctata
     6361 gaaaaagcta aggcagataa tatgccaaat gataatatag atagagctat cgctaaaggt
     6421 gctggagcag gtgctggaga agactatgaa actatagttt atgaaggata tggacctggt
     6481 ggagttgctg ttatagtaga aactttaact gacaacaaaa acagaactgc tggtaacgta
     6541 agatactatt ttgacaaaaa cggaggaaac ttaggaacta gtggatgtgt gtcttttatg
     6601 tttgataaaa aaggacaaat attagttggt ttaggagagg gagtttcaga agaagaattg
     6661 atggatgttg cattagaagc aggagctgaa gatttcataa cagaagaaga tggttatgaa
     6721 ataataacta caccagaaga tttttctagt gttcgtgatg agttaaaagc taaaggatat
     6781 gagtttatat ctgctgatgt gaagatgata cctcaaacta caactgtatt gactgaagaa
     6841 tctcatttaa aaatgatgaa taaattagtt gatatgcttg aagaagatga tgatgttcaa
     6901 gatatatatc ataactggga agtagaataa atagtataag catattgaaa tttataagta
     6961 ttgaatcaat tttaacatgt gataaatttt atagaataag aaacactaaa atagacatac
     7021 gcacctctaa cctatatttg cataataata tgtaagcata ggtaaggggt gtttttatat
     7081 ttataggtta agaaatttaa agtaaatttt gagctggact cgattgtcta taaataattg
     7141 atgggtatat aacttgttgc tgaataccat ttatacgccc agttactgta tcaataatca
     7201 tcctaagact acgttctgct gtctcttcca tttttaaatc tataactgtc aaaggaggaa
     7261 tacttcttgc cattagtcgt gaaggtccat tacttgctgc aaatatttga atatcctctc
     7321 caattacaat tcccgattca tgaaatgcat tcatcatacc aagagctacc acatcatgat
     7381 tacaaaaaat tacttttttt agtgaatttt ttgcaagcat tttttgagca atatcatatc
     7441 cactatctat tttattagaa caataatata tagaatctga tagatgtatt ccatgttctt
     7501 tacatgtttt taaaaaagat ttacccctta aagtcataca tgtaaaggaa gttgggttga
     7561 atacaacagc aatatcattt ttaccatttt gaatagcatg taaagctgct atacgaccag
     7621 tttcctcatt atcaatagat acacttgaaa aaccaggtat ttcacgattt acaacaacta
     7681 caggaatttt aggtttattt tttactagat attctatatc atcagcattg atacctgtta
     7741 aaacaattcc atcataataa ttatttgcaa aaaagtattc atcatcacat agatgaccaa
     7801 ctttgtaagg tcgtatgatg atatttatgt ttaaatgttc taaagaaatt atgtggttta
     7861 ttgcttgaag tatattggtt gatgccaact caaatccctc atcctgccaa taaaaagcta
     7921 ttttaatatt attatgttta ataggagtag agcgtttata tccaagctta tcagctgaat
     7981 ttaatataag ctcttgtgta tgtagcggaa tcctgcgttc atttccttta ccacttaaga
     8041 cgtacgagac agttgctgaa gaaagaccac agtcttttgc aatatctttg attgttgcca
     8101 tgataaaatc ctccttaaat agttgcttaa ataagcttat gattaacttg aattaagcaa
     8161 agttaagcag gaacatgcaa tgctagcttg caatgtgact gcaaaggggg aaaaggtatg
     8221 agtaaggtaa atatatttga agttggtcct agagatggat ttcaaaacct gaaagagttt
     8281 ttagaaactg ataaaaagct taagattatt gatggtctta ttgcttctgg gattaaacaa
     8341 cttgaaatta gttcatttgt aagcccaaaa gcaatacctc agatgaaaga tgcaaaagaa
     8401 gtagcaactt attgtgtaga aaaatatcct gatgtgaaac tttatgcatt agttccaaat
     8461 ttatacggtg ctaaagttgc atgggagtgt ggtataagag atatatccta tgttatatct
     8521 ttgagtgaaa ctcataataa aaaaaatata aatcgtaccc atgaagagtc tattgatgag
     8581 cttaaaaaaa taatgaatac ttatccagac atgaatatat gtattggaat ggctactgta
     8641 tttggatgtc cttttgaagg tttaccaaaa attgaagatg ccataagatt tgctaaacgc
     8701 ctttgggaaa acggtatcag ggagataaat ctagcagata caatagggat tgctgaccct
     8761 aagcaagtaa gagaaactat tagtgcatta gttgaagctt taccagaatg cagatggcaa
     8821 gtacatattc atgatactag aaatatggga atggttaata ctcttgtggc tattgaatct
     8881 ggaattacta ctgttcagtc tacacttgga ggattaggag gatgcccatt tgctcctgga
     8941 gcatctggca atactgcaac tgaagattta gtatatatgc ttgaacgtat ggggtatgat
     9001 acaggagttg attttgttaa acttcttgaa ttatcaaagt atcagaagag ttttgtaaat
     9061 ggagtatata gtggtcatca tgtaaatata aagtctgata gttgtaattg ttagttctat
     9121 tgattaatcg attaaaaaaa tatgttgatt aaaaaaatat gctgattggt aaaatagatt
     9181 tattgataaa aaatataaat tataaaatac attatctaat aaaacaaata aaaaaattga
     9241 gggggattag tatggatatt atagctttac ttttattttt aggagttatt attattgcat
     9301 ttgtacgtaa gaataatgca ggtattttgg ctctagctat tggtgtcata gctgttagaa
     9361 tttttggaat ggatgataaa gaactttatg gagctataag caactcaatg tttactacac
     9421 ttgttggtat aacactactc tttgcaatag tgaactcaac aggagcatta aagcttgttg
     9481 cagaaaaaat tgtagctgct tctggtaaac gtgtttggct tatacctata gcagttttta
     9541 tagcaggatt tgttgttgct ggtgttggac ctggggcaat tccagcactt gcaattatcc
     9601 cagcaatggc tgttcctgta gccataacgg ttggttataa tcctgtaatg cttgcactta
     9661 taggtgaatg tggattaatg gcaggtcgta tgactcctat tactccagaa ggtcaaatta
     9721 taacaaccgt tgcagaaggg gttggaattg aaaatgttat gcctactatc ttagcctgtc
     9781 aaactctttc tacaattgta ttttcaatag tattgtttat aattttcaaa ggatataaac
     9841 ttaaaaaacc tataaatgtt atagatagta agcaaataga aaaattcaat ataaagaaga
     9901 taatttctct aataagcata gtagttatga ttgcttttat cataattttt gatatgaatg
     9961 ttgggcttgc agcttttgca gtttcagctg tacttatttt tttcaatatt gctgatgaag
    10021 gagaatgtat caaatcaatt ccatggagca cgatagtaat ggttcttgga gtaggtgcaa
    10081 tgatgacaat cgtagatgca gcaggaggta ttgacttgat gagtaatgca ttatcatcac
    10141 tgatgaatag taaaacagct actccaatta tgagtatttc agcaggactt atgtctatgg
    10201 ttagttctgc tttagcagta gtttatccaa ctatgatgcc tatgtgtgtt gatatagcta
    10261 aggcagttgg aggagtagac ccactagcac ttattgctgc agttggtgtt ggaggttctc
    10321 ttgctggtgt aagcccactt tctactggtg gggcattgat tttagcagct atgggttcaa
    10381 gtaagaaaga ctttaataag gaagaacaaa ataaggtttt tgtacagctt ttcattatga
    10441 gtgcagttgg cttattggtt attgcagtag tatcaatatt atcatttaat gcaataacac
    10501 atttaatgaa ctaattagaa taactgaaaa taaatcatta aatttaactt ttaatattaa
    10561 tcaatataat ataatttgga ggggaagtta tgaaaaaagg agcattaagc aatataaagg
    10621 tacttgacct tacaagagtt cttgcaggtc catattgtac aatgatgttg gcggattttg
    10681 gagctgatgt tataaaaata gagatgccaa gaaaaggtga tgatacaaga ggttttggac
    10741 cttttgtaaa tggagagagt ttatactatg caaatataaa tagaaataaa aaaggtatca
    10801 ctctaaacct taaatcagaa gaaggaaaaa atatattttt agaaatggta aaacatgctg
    10861 atgttgtagt agaaaattac agaccaggtg taatggaaaa gttaggtctg ggttatgatg
    10921 agttggctaa agttaacaat caaataattt atggagctgt atcaggattt ggatgttatg
    10981 gaccatattc agaacgtcca ggttatgata ttattgcaca ggcaatgggt ggtcttatga
    11041 gtataacagg gcaagaagga ggagaaccaa cgagagttgg taatgctatg ggtgatgtac
    11101 taggtggaat gaatttaacc attggtatac tcacagcact caacgctaga aaaataatag
    11161 gaaaaggaca aagagtagat gtttccctcg ttgattctgt tgtatcgtct ctcgaaactg
    11221 gtatacagag atactttgat tcaggacaac ctgaacttat gggtaataga tatgcttcag
    11281 cctatcctta tgactctttt aaggcaagtg atggaagttt tgtaataggc tgtggtaatg
    11341 acaaactttt tgaaaagtta tgtaaaaaag ttctttttag agaagacctt ttaatagatg
    11401 agagatttaa aacaaatctt aatcgttgtg aacattacaa ggctcttaaa ccagaaatag
    11461 aaaaatggtc tgttaagcat acaataaaag aagcagttga cttaatatca gaggctggag
    11521 ttcctgctgc tcctatctat aatcttgatt gtgttacaac agatgaacat atagcaaaag
    11581 caagagaaat gattgtagat attagtcatc ctattatagg aaatatgaaa gtcaatggaa
    11641 atccagttaa gttgatgaca acaaaagcta aaattgatat gccagcacca tctctagggg
    11701 aaagtaacga ggagatttat aatggttggc ttggttttga tagtgataaa ataaaagatt
    11761 taaaagaaaa tggagttatt tgattaaaaa atagctaaac acacttgtga ctttagtcat
    11821 gagaggttca gttagaataa tatcaaaaaa tattaaatca atactgtaaa tcaaatatag
    11881 aattgtcaga agcatttaag atgggaaaag ctacttagac attgagtctt tgtggctttt
    11941 cctattttca ttaagaattt tatattatca gaatagatat gtactaaact aaagtattaa
    12001 caatttaaat taatagaaaa taaactacct aattatgttg aataaagact actttagtga
    12061 aaataaactt atagagatgc tatttaaatt attttaatgg aaatatcaaa aatgagactg
    12121 cattctcaaa aatgagactg cgttttttaa gtattttctc gaaaatgaga caaaaatatt
    12181 gttttatggg ttttattgaa gaaaaattct tctgtgaatt acaatttaat caattttaaa
    12241 gttgaaaatt attaaaaatt tagtgaaatt tatgtatatt tcgtttattt tattaaaaat
    12301 aacaattttt tactaaaaat aaagaacttc aatggtgatt ggcatagtac ttgctatttt
    12361 atattaacaa gaaatgtaga aaaaataaca atgtataatg gggggaatat gatggaatct
    12421 gtaatggaaa atttgaacaa tcttaaggtg gaggttaagg acaaagtctg tgttattact
    12481 ataaatagac ctaaagcttt aaatgctctt aatagtgaca ctcttaggga actatcacaa
    12541 gttattgatg tagtatcaga aaatgaggca atattaggag ttattattac tggagaaggt
    12601 aaagtttttg tagccggtgc tgatatacgc cagatgcaaa actataaaag tgaggaaggc
    12661 agaaaatatg caggttatgc tcagggaatt tttgataaaa ttgaagctct tgagaaaaca
    12721 gtaatagcag cagttaatgg atatgcatta ggtggtggat gtgaacttgc tatgagttgt
    12781 gatattagaa ttgcatcaga aaaggctata tttggacaac ctgaagttaa tttaggagtt
    12841 attccatgtt ttggtggaac tcaaagatta tcaagacttg ttggaactgg aatagctaaa
    12901 gagttaattt ttacagggag acaagtaaat gcagaagaag ctaaatctat tgggcttatt
    12961 aataaagtgg ttccaagtga tttactttta gaagagtcta tgaagatgat gaaccaaata
    13021 gttgaaaaag cgccaatagc aataagatat gcaaaagttg ttattaacaa agggatagat
    13081 atggatttaa agaatgctct tgaactagag aaagatattg caggtttaac ttttgctaca
    13141 agagataagc aagaaggaat gaatgcattt atagaaaaga gaaagccagt ttttgaaaat
    13201 aagtagctat tataactaaa attggaacaa tgtagaatat attcaaaatc taaatttcgt
    13261 gaaaaaatac atatagttta attttaaaag gagttaattt atgaatacgg gagtaattat
    13321 aagtttagta gggattatca tagctctatc tcttctagtt gtaatggtta tgaagggaat
    13381 aaatattttt attatagcaa ttgtatgttc atcattagtt gctataacag gaagcctaaa
    13441 tgtgtatgat gcacttaaag tggattatat gacagggttt gttggatttc ttcaggcaaa
    13501 tttctttatt ttcctaacag ggacattaat gggaaaaata atggagatta ctggtggagc
    13561 aaaatctatt gctaagatga ttgtaagatg gattgggaag gataaagcac tactttctat
    13621 tccaatagct tgtggaatat tagcctatgg tggagtaagt gttttcgttg taagttttgc
    13681 agtgtttccg atagctttag aaatttttaa agaagctgat ttgccacgaa gatttatacc
    13741 agcagctttg acttttggat gctcaacttt tgctatggtt gcaccagggg caccacaaat
    13801 tcagaatatt gttccagcat caacattagg gacagatatt atggctggag cagtaaatgg
    13861 ttttatatca tgtgcagtaa tgtttatagt tggaagtacc attttgtaca aaatggtttc
    13921 aaaagaaaaa gccaatggtg gtcattttat agcacatgaa tcagatatat ttgatgaagc
    13981 agaagcaact aattctaaga aagaaaatgg tcctaatggt gtaattgcct taattccatt
    14041 gatagtgtca attttaataa ttaatgtaaa agttaatgga aaagcgatag taccaattga
    14101 ggtgggagta tttattggag ctttattagt atatttattg ttaaataaat atcaagataa
    14161 tactaaaata gttggacatg ttggtgatgc atgtaaaact acagtagtag caatatgtaa
    14221 tacttgtgct gtagttgcat ttggttcagt agtaaaatca gcagttggtt ttgacttcat
    14281 agttaatgcc atgactaata ttcctggtcc tcctattgca ggagctgcag ttggaactac
    14341 agttattgca ggtatttgtg ggtcagcttc aggtggacta ggaattgcag ctccacttct
    14401 tggaccagta tttttagcca agggtgtttc cgctgcagca ttaactagga ctatggcaat
    14461 ttcatcagca gcattagatt cacttccaca caatggatat attgttactg taacaaatgg
    14521 tctttgtaaa gaaacacata aggatgccta tatgccaata ttctgggtaa ctgttttaac
    14581 cccatttatt ggaaccatag tagcagtcct actgtttaca atgttcccgg gattgccata
    14641 agagaaaaga taaaaggtat aaattatatt ttgatattat aaaatcaatt ttcaaaatat
    14701 atacaataaa tttcatgttt taatactgta aatatgtatt taaaataaca aattgaccat
    14761 ataaggagga attgaaagtg ttaaataaac cattagaggg agttaaggtt attgacctaa
    14821 cgtattttgt agcaggtcca gggacttcta aaatattagc tgactggggt gctgacgtta
    14881 ttaaggtaga gccaagcttt ggagacccag gaagaaagac aggggcaaca atgacaatgc
    14941 caatagatga ttataataat cctttttaca gcacatataa ttcaaataaa agaggtttgt
    15001 ctataaatct taagagtgaa actggaattg aaattatgga taagctttta agcgaagcaa
    15061 acgtatttgt ttcaagttat agaacaggag cgttaaaaag actaggtctt gattatgaat
    15121 cattaagtaa aaagcatcca caccttatct ggggtcagat aaatggtttt ggcgattttg
    15181 gacctgctaa ggataatgca ggatttgata cagttgcctt ttgggcaaga agtggggcta
    15241 tgcttgatat agcagaaaaa gatacaagtc caataaatcc acctatagcc tttggagatg
    15301 caacaacttc ttgtagttta tctggaggaa tatgtgcagc tttgtaccag caagctaaga
    15361 ctggaaaagg acaaaaagta atggcatctt tatttgggca agcaatatgg aatgcatcat
    15421 ctttattagc atctacacag tttagtgatg agtatccaaa aactagaaaa aatgctattt
    15481 caccagtaat aaattcttat cgttgtaagg atggaaagtg gatattctta agtattttag
    15541 agcatgaaag atattttgag gcactttgta gattatttgg aagagaagac ttaataaaag
    15601 aagagaaatt tgcatcttca gtagaaagta agaaaaatgc tacagaactt attaatattt
    15661 tggatggtga atttgcaaag tttactcaaa atgagatggt tgaaaaactt actgctgctg
    15721 atatagctca tgaaaaaatt caacatgtta atgatgtagt gactgatgaa caggctattg
    15781 ctaataacta tatatatgaa catacaagta aaaatggaaa taagacaatg cttgcatcta
    15841 ctccagttaa atttggaaat attgaagtta acatgacttg tgatgctcca cttataggag
    15901 aacattcaga tgagatattg agagaacttg gatacagtga ttctgatata gaagctctaa
    15961 ttgaatctgg aattgtaaca atgaataaag aagcatgtga aatatgttaa taaacatgtg
    16021 aagtatgtta ataaatagga gggaatttta tggattttag attaacagaa gcacaattaa
    16081 tgcttcaaag agtagcaaaa gaatttgcag agaatgaaat agcaccaata gctgctgaaa
    16141 cagataaaac aggtattttt ccaagggagc tttttagcaa aatggctaaa ataggattta
    16201 atggaattgg aacacctgta gagtatagtg gttctggtgg ggcagatata gaaaaggtaa
    16261 ttgttgttac tgagattgcc aaaaaatgtg cagcatctgc agcaatatta tcaatacaca
    16321 caatatatgc tcaagctatt ttgaaatttg gtactgagga acaaaagaaa aaataccttc
    16381 caatgatggc tgaaggtggg tgtgtaggtg cttttgcctt aaccgaacct aatgctggtt
    16441 cagatgctgc aagagctgca acaactgcaa ttattgatga agaaactgat gaatatgttt
    16501 taaatggaac taagtgtttt atttctggtg gaggtcaagc agaatcatta ataatatttg
    16561 cactgacaga cccatcaaag ggaataaagg gaatgtctgc tatcattgtc gacaaaggta
    16621 ctccaggttt ttcgatagga aaaattgaag aaaaaatggg tattcatggc tcagaaactg
    16681 cagagttaat atttgataac tgtagagttc caaagtctaa cttattagga aaagaaggca
    16741 aaggatttaa tatagctatg acttgtttag atggagcaag aattggagtt ggagctcaag
    16801 ctgtaggaat tgctgaaggg gctttagaag aaagtatcaa atattctaaa gaaagagtcc
    16861 agtttggcaa gccaatttca gctttgcaag gtattcaatg gtatatagca gatatggcga
    16921 caatggtgga atcagcaaaa ttattagtat attatgctgc agatttgaaa gctagaggag
    16981 aaaaacacac taaagaagct gctatggcaa aatacaatgc gtcacgtact gcaagagagg
    17041 ttacaaattt agcccttcaa atccatggtg gatatggata tatgaaggat tatccattag
    17101 agagaatgta cagagatgcc aaaataacag aaatttacga aggtacttca gaaatacaca
    17161 aagttgttat ttcaagagca gtactaggat aggaggaaaa gaatgaatat agttgtttgc
    17221 ttaaagcaag ttccagatac taatgaagtt aaaataaata aagagacagg tactctaata
    17281 agagatggtg taccaagtat aatcaatcca gatgatagaa atgctttaga agaagctcta
    17341 aaaatgaaag atgaactagg agcagtgatt aaggttataa gcatgggacc tcctcaagct
    17401 aaaagtgctt taaaagaagc tttggcaatg ggagctgatg aagcttattt aatctcagat
    17461 agagcttttg gtggttcaga tacttgggct acatctacaa ttattgctgc tgctatagag
    17521 aaggttggaa agtatgatgt aatattctgc ggaagacaag ctatagatgg agatacagct
    17581 caggttggac cagaggttgc agaattttta gggataccac aagttactta tgcgaaagaa
    17641 gtaaaggttc aagatgacaa attactagta actcgttata cagagacagg ggattattta
    17701 atagaagcaa agatgccagt tttattaact gctataaaag agttaaacaa tccaagatat
    17761 ccaagtgtca aaggaatctt agaagcttat aataatggag atgcaaagat tactgtatta
    17821 acacttgcag atttagatgt tgatacaact caaattggat taaagggttc acctacaaac
    17881 gtttataagt cttttgtacc agttaaagac aagcataatg aaataataga aggtataaat
    17941 aaaaaagaaa aagctgagaa actaatagaa atactattcg atttgaaact agtataggag
    18001 tgttaagatg atgagagcaa aagttaatca aggtataaat ttaaatgatt ataatggtgt
    18061 ttgggtaata ggagagcaaa gggaaggtaa aattaatcct gttacgatag aacttattgg
    18121 tgaaggaaga aagcttgctg accaattagg aaaagagctt gcagttgtga tagctggata
    18181 tgaagtagag aaagaagtga aggaattact tcactatagt gttgataaag tatattacat
    18241 caatgaccct cttcttaaag atttcactac tgatggatat gcaatttcaa ttgcaaattt
    18301 aatcgaaaga aaaaagccag aggttgtatt ggttggtgct acttctatag gaagagatat
    18361 agcgcctaga attgcaggta aggttggaac aggtctaact gctgactgta caaaacttga
    18421 aatagattca acagataata agttattaca aacaagacca gcatttggtg gaaatttaat
    18481 ggctactatt gtttgtccaa aaaatagacc acagatgtca acagttcgtc caggagttat
    18541 ggcaaaagct gtaagaaacg aatcagagac tggaatttta gaagttgtta ctccagaact
    18601 tactgaaaaa atgattagaa ctagattagt agagatactt ccacaagaaa agaaatctgt
    18661 aaatctaact gatgctagaa taatagtatc aggaggaaga ggattaaaaa gagcagaagg
    18721 atttgagctt ataaaagaat tggctgataa attgggggca gagattggtg cttcaagagc
    18781 agcagtagat tcagggtgga tagaacattc tcatcaagtg ggacaaacag gaactactgt
    18841 tagaccagaa ctatacattg catgtggaat atctggagca atacaacatt tagcaggtat
    18901 gagtgattct aaatatatag ttgctataaa taaagatgca aaagccccta tttttagtat
    18961 atgtgactat ggaatagtag gagatttata tgaaattata cctgaaatga tagaatcatt
    19021 aaataggtaa cttttagaaa acattgaata tgtagaatga taagtcttat aatattgtat
    19081 tgtaagactt attatttttg ctgaaggtta tagtggggag atgaaacaat gaaagagcag
    19141 tggtataagg atatatttgc aagagtatta tctatgactg atgatggttt tattgttgta
    19201 aatacaagtg gagtaataat agatattaat gataaatatt gtgacttttt aggaaaagaa
    19261 agaaaagata taataggaca aaatatacaa agtattatac caaatacaaa aatgctagat
    19321 gttatgaaaa ataagtattg tgaagaagga gctatacatc attattcagg tggaaataca
    19381 aaagaaaaaa gtgtaatagt aagtaggtct tatgtggaaa atgacaatgg agaagtagtt
    19441 gcaggagtag ctcaagttaa atttagattg caatcatttg atgtagctaa aaagttaatg
    19501 tcagaatata tggagctcca atattataaa gagcagttta aggataactg tggttttgac
    19561 aagctcatag gtgaaaatag agacttcata gaacttaaga aaacaggtgt gaaagcttct
    19621 aaaacaaact ttccagtact tttaacgggt gaaacaggca caggtaaaga agtttttgct
    19681 agagcaattc acaataatag cagtaggtca gataaaccta tggttagtat aaattgcgct
    19741 gcaataccag aagaattatt agagtcagag ctatttggct atgacgaagg agcttttaca
    19801 ggagcaaaaa agggtggtaa aaaaggtaaa tttttagttg cgaataatgg tacaatcttt
    19861 ttagacgaga taggtgacat gcctttgacc atgcaagcaa aacttttaag agttcttcag
    19921 gaaagtgaga tagaaccagt gggaggtctt aaaactataa agatagatgt aagagtaatc
    19981 tctgccacaa gaaagaattt aagtaaaatg gttgaagagg gattatttag agaagaccta
    20041 tactatagat taaatgtaat aaacattcat atgatggaat tgaaggacag gcaagatgat
    20101 attttattgc tagcaaatta tatattaaac aaattaaatg ttgaatacaa ggaattgaag
    20161 gttttaagtg ataaggttaa aaattgtttt attaactata cttggccggg aaatataaga
    20221 gaactacaaa atgtaataaa aagtgcctat gctgtaagtg atgatatggt cattatgatg
    20281 tgtgaccttc catctaagat ggataatatc agcagagtag ctcaatgtaa tgtggatagc
    20341 aattgttcaa ttcatgaaat ggttgagaat tatgaaaaga gcttaattat tgatgtgtta
    20401 aggaagtata attggaaatg ttctaaggca gcagaggtta tgggaataca caagagtctt
    20461 ttatataaga aaataaaaaa gtatgaaatt gaattaaata attaagataa cttgaattaa
    20521 ggcaatgcta aaaaacgccc ttattcaaag cgaactgtat aaactgtttt atgcagttaa
    20581 aacgtgtaaa agtagatgtg aagtactaat gtcaatcatt agtacttttt tattatattt
    20641 ataattacta tctatatttc agtatctttt gatatctaag attattatgt ttaatttagg
    20701 atataacaaa accctcttag ttatggacag ttttttatat tttaactgtc tactaagagg
    20761 ggatagttta gtttaaattt agagtaaatt ttatctagca tttataaaaa atattttgaa
    20821 tagctttttt gttatttaat cataattttc tacttcttta tcaagttctt tgccacaaga
    20881 atcacactta tatatagcat tgtatccgta atcatattca tcaactattt tcttttgtct
    20941 aaatacacca ccacactcac ataggaaacc aatttttata agtgtaacga taaattgtga
    21001 ttttataaat tttatatcat tacatttatc gttagtgtaa aatgtataaa tataagagtt
    21061 atcgcactca tctcttaatt caaatctttc aatatatgtg ttatttttta attcctcaaa
    21121 ttcttcttta gttaaatatg atttttgttg caattcatgt aaacgcttaa tttggtccat
    21181 tatttatatc ctcccaataa ttttagtatt ttaagcttag ataaggaatc taagttttat
    21241 taaatttatc ccaaggaatt atttaaatca tatttagttt tattaattac aacatatata
    21301 aattacacga aaataaaact aattgtaaaa ataatatcct gtgaaaacct aaactactaa
    21361 ccccttacta acccgaaaac taaaaaaaga aaatatatcc aaatataagt gatatataaa
    21421 taaagatgtt tcatataaat ttaactttaa atattctgac aataatatat ataaatatat
    21481 tatagttaaa atataccaca tattttgggt attgtatact ggaattagag taattaattt
    21541 attttttaac atacaaatga ttttatgaaa aaaaacaaga ctcaaaaatt tttatattct
    21601 ttgatagaat aaaattgaga gtaaatttat tgaattttat aaaatttata aggggtgagt
    21661 ctaagatatg catgaagata aaaatgatat atttgaatca atagatactg aggattttga
    21721 taataaaaca gaagaagaat gtttagagct agtaagaaaa aatggattga atttaatata
    21781 cataaaaaat caaacagaag agatatgtct agaggctgtt agacaaaatt gtaatgcaat
    21841 aaaatgtgtt cagaatcaga cagaaaaaat atgtatagaa gcagttaaac aagattggag
    21901 aatgttagaa tttgtagaag agccaacaga agaaatttgc atgttggcta ttaaccaaga
    21961 tggaacagca ttaaaatata tagaaaatca gacagaggag ttatgcttaa gagccgttga
    22021 aaaaaatggt gcagctttgg agtatgtaaa agaacaaaca gaagaaatat gtatagaagc
    22081 agttagaaac agcgaatttg gattggcaag agtgaaaaat caaacagaaa aaatatgtat
    22141 ggaagcagtt aagcattgta gctataatct aaaatgggta gaaaatcaaa cagaagaaat
    22201 atgtatggaa gcagtgagac aaaatgggct tgatttgaaa tttgtaaaaa atcaaacaga
    22261 aacaatctgt ttaagagcag taagacaaaa tggaatggca ttagaatttg taaaggaaca
    22321 gactgttggt atatgcttaa aggcagtaag acaatatgga atggcattaa aatttgtaaa
    22381 agaacaaaca gaagaaattt gtacagaagc tataaaacaa gataaaagag cacttagttt
    22441 tgtaaaagga gataaggaaa aatacaaggc tctatatgat aataatgaac catttgcaaa
    22501 gagatatgtt agaaatgtaa tagaaaaaga aaacagagca ttgattaaaa aaggagaaga
    22561 aaatgcaaaa atcaaaattg caggaaaact ttatgataga ggcatgtctt ttgaagatat
    22621 atcagatata gttgagattg aaataagcaa gttaaaagtg agtttaggtg ttttttaaag
    22681 taataaatgt aggtgttttt tatgggaaat agttaataaa aaaataatcg actagctctt
    22741 tgtaggacag caaaattagt gatataattt gatgtataat aaattatttt tattagataa
    22801 gggggaatta aagttgaaga ttacagtatt ataccaaaca agaagtggaa acacagaaag
    22861 ggtcgctaag ctgatagaag atggagctaa aaaggtggat ggagtagatg taaagcttat
    22921 gagacttgat aatattgatt tagattattt aaatgaaagt aaagcagtaa tatttggaac
    22981 acctacttat ttagcaaata caacttggga agtaaaaaaa tggtttgatg aagattcaaa
    23041 aaaagttaat ttagctggaa aattaggagc tgtttttgct acatgtgatt atatatgtgg
    23101 aggtccagat gttgcgatac ttactatagt aggacatttg atggttaaag ggatgcttgt
    23161 gtattctgga ggtggttctt taggaaagcc atttatacat ttaggacatg tacattcaat
    23221 tgaaggtcca gagttacaag atgagaaggc tataatattt ggtgaaagaa ttgctactaa
    23281 ggcaaaagaa ctatttgctt aatttctata tgcataataa tgattagagt aaatttaggg
    23341 ttttaatgga gatttgaagt aaaagttata aaataaaaaa ttttccataa aattactcaa
    23401 gatgaattat aatataatta taaataaatt atattattaa aatatttaaa atcaaacgtc
    23461 aatagcgagg gaagtttatg gtatggcata tagaatatga gattttttct gcaattatag
    23521 ttattttttt aatggtatac ttttttagaa gtaaatttat acctacttta caaaacaaaa
    23581 tatattgtgc acttttaata ttctcatttt tatttataat ttcaaatatt ttaggttcat
    23641 tttgtctaaa taatatagac aaaattccaa tttttattac ttttttattg aatcagatat
    23701 acttattgtt attgccaata ccagcagcac ttgtatcatt ttatgttatg tctattattt
    23761 atcaagatat aaggtatatg aaaaaaagat tattattttt atctatacca cttataattt
    23821 gtataatctt atctattaca aatcctttta cgcatattct atttggattg tcaaaagaaa
    23881 caggttatat acgaggttgt ggatatgttg caacttttgc atcttttttc ttttatgcaa
    23941 tatattcagc atttttatct tttaaatata gaaaagcaat gcatgaatca aaaatatggg
    24001 ctattagagg ttttcttata gtttcaattg tggcaattat cttacaggca atttttttac
    24061 agtatcttct tacaggaact gcatgtgcat gtagtttact tttgacatat ctttctatgc
    24121 agaatcgagg tcttattatt gatgacctta ctggtgtact caatagacaa tcttttattc
    24181 aagagctaga tttgaatatc aatactgaac aatcaggatt tattattaca attgcgcttg
    24241 atgattttaa gtttatgaat gaaacttttg gaacaaaaaa tggagatatt gcactaaaag
    24301 aagttggaaa atatttgatt cagatacatg atgtaaacca tgtatataga atagggggag
    24361 atatattttc tattgttgtc aatagtcaat taggtataga accagatgat attatcagaa
    24421 agatagaaga aagatttaaa aagccttggc tagtagaaga gataagtttt aatttagcaa
    24481 ctagtattgc tgttgtttat tacccagaaa atgcagaaac gacagaagat gtaattgttg
    24541 ctatagattt ctctatacat gaagcaaaac gttctgagtc aaggcgagtt gtttatgcag
    24601 atgcgtctat aagtgaaaag ataaagaaga aacatgttat taaaaattgt ttaaaagaag
    24661 cattaaaaaa tgatgggttt gaagtgtact accaacctat attttctaat agcaggggca
    24721 ggtttacttc agcagaggca ttacttaggc tagaacacaa agagttaggt tttatacctc
    24781 ctgatgaatt tattccaatt gcagaacaaa caggattgat taactcaatt ggattagtag
    24841 tctttgagaa ggtatgtaga tttatagcta gtgatgagtt tgaaaattta aaattggata
    24901 atatagctgt gaatctttct gtagtacaat gtatgcaaaa aaacttagct gatgatttac
    24961 tttatcttat gaataaatat aatgtatcac caactaaatt taaacttgaa attactgaga
    25021 ctgtggctat gggctctttt aatataatta aagaaacaat ggaaaggtta attgatttag
    25081 gtgttaagtt tgcacttgat gattttggaa ttgcttattc tggagttact aatatgcttt
    25141 ctttaccatt ttcagtaatt aagttagata agagtttgat ttggtctatg aatgaagata
    25201 gtaggcataa acttacagtt gagacgatta tagcacttat aaataaattg aatatgaagg
    25261 ctgtagcaga aggtgttgaa accattaagt gtgctgaaga tttaatagct atggagtgtg
    25321 aatatttgca agggtattat ttttctaaac cagttccaga agatgttttt aaaaatttgt
    25381 tgaaagaaaa tacttttaca agttaacacc ctttattttt ttaattacaa ctttgttcat
    25441 tgacactgcc agcgatatat tataaaatta acacaataat aaatattaaa tttattatac
    25501 atattattaa tattaattaa tggagtaaag atttattata tattttattt aattatccta
    25561 aagtgagaaa ggaagatgtg acatgaataa aaagaaaata ttagtgccaa ttgatgggac
    25621 agaaagaagt atgcattctt tggaatttat aaaaggaata tttaaaaaag atgaagttga
    25681 agtagaaata atgaatgtaa aagaactagt atttattgat ggcatatccc tggctgaaga
    25741 gataaaaaat tctgaaaatt taggaagaag aatcttggat aaagcagcag agataatggg
    25801 agagtatgat gttaaagtac attttacttt tggatatcca ggagatgaga taattagaaa
    25861 agcaaaagaa gataatgtag attttatagt tatgacaaaa tctactaaaa aaggtcttac
    25921 aagaatgata ggttcagtaa cagcaagtgt agttaaacaa gcaaaatgta tagtgatgat
    25981 tgtaccggag tagaattaga aaggtatttg aaatatgaat aaaaagatag tagcagtttg
    26041 tgaaagtgaa agaagtttaa attctcttaa gacagctgca aatgatttag gatttgattt
    26101 agtttatgaa attcaagaaa atgataatat aaaaaatgaa ctttctattt atgatataga
    26161 agatgcatcc atagtgttat ttgttacaag tgattctata gaaagcatag agaagattga
    26221 tagatttata gatagagaat tttatgaagt tgatccaaag tatataatag aggatgcaaa
    26281 atctatagta agtgaaattt taatagactt aaattaatcg tgggtgtcgc actaagaata
    26341 gagcgacgcc tttttatata ttttacacaa gaaataaaga ggttgtatca aaatatagat
    26401 aagactcttt ttcattgttg aaatgatgaa aatatatttg atttcataca aaaaagagta
    26461 cctcataact aaaaagttca ctttgagaca ctccattttt atatgttagt tgcatttaag
    26521 gtaaaatata agtttaatca tgttgaaata actgaattac atcttctaaa tgattgtaca
    26581 ttgatgtata acctaagtaa gagtctttac tatcaaatgc aatcattatc ttgttatggt
    26641 ggctaatcat atccttgaga gaatttctat atttaaaacc gaagtattgg ttagaagaaa
    26701 ctgttttagg tattaactca taatatttta taaaaattgg attatgagaa gctaaaatta
    26761 tctctttgtg aaattgcaag tcagcatcat aacattcttc tatcaaacca tgagaaacct
    26821 tatccaattc ctctattgac tttaggtaag tatattttat tttttgaagt tctttagatg
    26881 tagcatttaa aattgctagt ttagtagctt cgctttcgat aagttgtctt gctcttaaag
    26941 attctacata aaattcttgt atgttatgat ttttatagat ttcaccatca tcaaataggt
    27001 caactttttt aaatccttca actataggac ttgaataagg cacagctcta agtattcctt
    27061 ccttacagag tatatttatg gcatttctta caggtattct acttatgtta aataggtcag
    27121 caatctttct ctcacttggt aatttactat taattttaaa agttccattt agaatgttta
    27181 atttaatata atcaataatt ttttcagtgg catttaagct tttcataaaa actcctaaac
    27241 atataatttt tttatgattg tattatatca tagttaagag ttaaatctac ttttttataa
    27301 tatgtataag acaggataga ggccaaaata gctataatag ccaagataaa gtatccatat
    27361 tgtattgaag tatattccga tattttacca actatcattg gaaatacaga cattcctata
    27421 gcataaattg cttgaaaaaa acccattgca gtagatttct gatgttttgg tacatttttc
    27481 atagactcgc tagtaaggta agaaaggagt atacctgtag atatacctgg tagtacttgt
    27541 agtacaaata ttatattaat attttttatt gttggaacta aaaagcaata ggctgaaact
    27601 aaaataaaaa ctaaaggagt ccaaaatttt ggtccatact tagtacaaaa tttagtagaa
    27661 gcaaagccag aagaagcaac agcacacacc atgtaaaata tagaagatag acctatgagg
    27721 gtagaactag aacctaagtt tttaagaatt tgagtagtaa atgacattgt tgtagacatt
    27781 tgaataccct gttgaattaa tgccataaag gaaaaaaata caagtctttt atccttgcag
    27841 atagataaat attttttaat tgatattttt tcaggtattt tttttgaatt atgatttttt
    27901 atatttaagg ctagaatcgt tccaataact gaagacaata cactcatgat acataaggct
    27961 gtcattccta atttttcata aaataaagta ctcataacaa atccaatcaa cattcctaga
    28021 ttgtttacca atattatcgt acttgtagct ttttgttgat gttctgtaga aaaaaagttg
    28081 gtatagtgaa ccatgtaaga aatccaagtt gatgatgata ttccagataa aatgttagct
    28141 ataagaaaac cattgcctcc aggagaaaag actcttataa gagatgctaa accagcaaag
    28201 aaaattccag acagtataaa tattttatgt ttatttatag aatcagcaaa tactccaaca
    28261 ggaagcctta ataaacattg tgatattcca tatgcaccta ttattatacc aatcatattt
    28321 gtagatacat tacatgcact taaatatgga ttttggaaag gtatatatac atattgtgaa
    28381 aaccagaaaa atgtaacaat agaaagtaaa agtaattttt gattagatat ttttttcata
    28441 ttatcctcca tatggtattt ttataatgca aataatatac catttagatt ttttaatcaa
    28501 gtactttctc tattaaaaaa aatttaaata tataattatt atttacaaaa aaattatact
    28561 ttatagttga attaagaaaa cgcttatgat actataaagt taacaaattt gaatatataa
    28621 cagtagacgt gttactggta atgcaggcaa gatcgaatca atttttatag attatctata
    28681 gagtactatg ggtataatct atttatttgg ttcgattttt ttgtttaatt ggccataaaa
    28741 caaaaagcct atagttgaaa atggaggatt ttttatggat tataaaaata tagctcaaga
    28801 aattctacta aatgtaggag gaaaagagaa tgtaaatgaa gtgactcatt gtatgacaag
    28861 acttagattt aaagtcaaaa gcgcttctaa agtgaataag gataaattat ctaaaactga
    28921 aggagtaata actgttgttg agagtatggg tcaaatacaa gttgtaattg gaaataaagt
    28981 aaaaaaagta tatgatgagg ttataaaaat tatacctcaa tcaaataaca taggtaaaaa
    29041 gaatgaaagt caagaaaagc agggtataat taattctata ttatcagcag tagctggtat
    29101 atttacacct acaatacctg ctatagcagg ggttggtatg attaaaggta ttttatcagt
    29161 tcttgctatg tattatatga ataaaaatgg agtagatata aaagaaacgc aaagctatat
    29221 tatcttaaat gccatggctg attctatatt ctattttatg cctattatac taggatatac
    29281 tgcggctaaa gtatttaatg caaataaaat aatatctatg gtattaggag ctacactttg
    29341 ttatccaact tttactgctt tgatggcagg tgaagaatct gttagatttt taggattggc
    29401 agtaacaaaa gctacgtata catcatctgt tattccaata attattgcta tatgggctct
    29461 ttcatacgtt gaaaaagtat tagaaaagta tatacctgag gtaattaaaa taataatggt
    29521 tccaacttta tcattggttg taatgttacc tgcaacatta tttttatttg gaccaatcgg
    29581 aatttatata ggaaatgtca taaacttttc ttataagtat atttatgaat taagcccagc
    29641 attgtgtgga gcatttgtag gtgggttatg gtgtgtgttg gttatatttg gtgctcatag
    29701 agcattatta ccaataggta taagtgatgt tgctcaaaca ggaagacaga atttattggc
    29761 gtttgcagga gctgcaaact tttcacaagc aggggctgct ctaggagtat tctttaaaac
    29821 taaaaatcaa ggactaaaaa caatttctat gtctgcaact ataacagctt tatttggtat
    29881 aactgaacct gcaatatacg gtgccaatct tagattaaaa aaacctatgg tatgtgccgt
    29941 aatatgtggt gcatttggag gtgcgataat gggaatgggt ggagcttatg gtaatgcatt
    30001 tgcaaaccaa ggtgttttaa ctatacctgt atatgctgaa gcaggtgcat taggtttctt
    30061 aagttatcta ggtggttgtg ctatagcatt ttttggttca gctatttcaa cttatctagt
    30121 tggatttgaa gatttggaag agtcttctaa taaggaaaat tcatctataa aagtagaaac
    30181 gaaggatggt gttattgata taacttcacc tgtagagggc gaatgtatag aattgtcaga
    30241 agtaaaggat gatgtatttg cttcaaaagc tatgggagaa ggtatagctg tgttacctac
    30301 aaaaggagta ataacagccc ctactgattg tgaagtagct tctttatttc caacattaca
    30361 tgcaatagga ttaaaattag ataatggagc agaaatgtta atacatgttg gaatcaatac
    30421 agtagagtta aatggtaaat actttacaaa acatgttaat caaggagatt tagtgaaaaa
    30481 aggtgataaa cttatatcct ttgatataga taaaataaaa aaggctggtt atgatgtaac
    30541 tacgccagtt atagtaaaca atacatttga ttttgggcaa gtagtttctt gtaaatctag
    30601 ttatgtttca accaatgata atataatttc attagtatta gctggtaatt aagttaattt
    30661 gactagagat aagtaaattg atttaaaagg aaataagtaa atcaacaatt aaatacatct
    30721 aaaagtaagt ttattgcaat gaattgttaa agaaaataat taatatggag taaatctatt
    30781 gcaatgaagt aacaaagaaa ataattaata tagagtaagt ttattgtaat gaattgttaa
    30841 agaaaataat taatataggg taaatctatc tcaatgaaat aacaaagaaa agagttaata
    30901 tagagtgaac ttattgcaat gaatcaacaa agaaaataat caatataaaa aggagaaata
    30961 tttatgaata caggatttcc aaaagacttt ttatggggag catcatcttc tgcatttcaa
    31021 gtggaaggag cttgggataa agacaataaa ggaaaaaccg tagctgacta taattctttt
    31081 aaaaagtctc atttacaggc agatactaaa gtcgcatcag acttttacca taattatgaa
    31141 gaagatattg aattaatgaa agaattaggg atgaaaacat atcgtttttc aatttcgtgg
    31201 gcaagaataa taccagatgg tgaaggagag ataaaccaga aaggtttaga cttttataat
    31261 aagataatag acaaactaat agagtgtgat attgagccat tcgtaacttt ataccatttt
    31321 gaccttccat ttaaattagt agagaaatat aatggttggg aaagtagaga aactgtctat
    31381 gcttttgaaa gatttgctaa aatatgcttt aagcattttg gagatagggt aaagtattgg
    31441 caacctcata atgaacagaa tttaattgtt agagtggagg agagaattaa tatatatgat
    31501 gaaacagatt catggaagat agataaaatg cgtgttcaga tggattataa tctttgctta
    31561 gcacatgcat tagcagtcaa tgcatgtcat gagatgataa aagaaagtaa aattggagca
    31621 gcagtatcat catctgtaac atatcctctt acttctaagc cagaagatgt atatgcagct
    31681 agaatgaatg ataattttaa agtgtactat atgcttgata tgcatcatta tggagaatat
    31741 ccagggtatt atatgaagta tcttgaaaaa agaaatatag ttcctcacat ggaagatggg
    31801 gataaagaga tacttaaaaa agctaaaatg gattttatag ctataaatta ttataggaca
    31861 aattgtgcag aagctttacc tgaagattca caacatacat ttggattaag agaaggaact
    31921 gttgatttta gtatgtatgg actatttaaa atgtcaatga atcctaactt agaggcttct
    31981 gagtatggag cagcaataga cccatcaggt cttagagttg ccttaaatga atattggcaa
    32041 agatatcact tacctgttat aataacagaa aatggtctag gagctaaaga tatattagaa
    32101 gatggaaaaa ttcatgatga ctatcgtata gactatttaa gaagtcatat aaatgcatgt
    32161 aaactagcta ttgaagatgg tgtagagatg attggatatt gtccatggtc atttacagac
    32221 cttttaagtt cttctcaagg ttttaataag cgttatggac ttgtttatat aaatagaact
    32281 gaccatgaaa ttttagattt aaaaagaatc aagaaagata gtttttactg gtataaagaa
    32341 gttatagaga acaatggaat agtaaaataa acataaatat atattgaggg ttttagtatg
    32401 tacattacaa aggtattaaa taattcgttg ctacttgcaa aggatgataa tgatgaagaa
    32461 ataatattga tggggaaagg cattggccat aattataagg ccgggtctga gttaaataaa
    32521 aaagatatag aaaagatttt tgttcttcat gatgaaaata taaaaaagag tattatacaa
    32581 ttggcaacag aaatagatgc agaatatttt ggtatagctc aaatgataat tgcatatgga
    32641 atagaaaaat ataacttaaa acttatgaac catatatatt tggccttaac tgaccacatt
    32701 gcctttgcag tgagacgttt taaagaaggt ataatgattg aaaatcacta tttgtttgaa
    32761 atcaaggaat ttaatccaaa agagtatgat ataggaaaat atgctattac tgtttttaag
    32821 gaggttcttg gtttagacct accagaagaa gagattggta atatagcagg ccattttata
    32881 aatgcacaac aagataatcc ttatagtgat agaaataaga gaagtgctaa aattgtaaat
    32941 gctattttgc aaatagttca ttatcacttt tctattgtat ataataaaga gtcattttat
    33001 tataggagat ttgtaatgca tctaaagacc ttttcacaaa gatttctggc taatgaacca
    33061 tcaagagaaa aaatagattt tatatatgag caagttcaaa aaaattgtaa ggaagagtat
    33121 gaatgtgtga aaaaggtagg agcttatatg tataaggaat actccagaga attaccaaga
    33181 caagaggagt tatatttgat gattcatatt cataagattt taggagaatt atgcgaaaat
    33241 agttagaata tcttaatatg ctagtaatat tataaaaaat taaaaattaa cgacacttta
    33301 aatatgaata attgcattac ttaagggggt tgtcttaaaa ttgatttttt taattaaatt
    33361 tgaagctcaa ctcctttttt aaacctttat atcattacaa ctttaatgtt tatacgcctg
    33421 tactttagat gtatatctac tttttacatt tctcaacatg caaacctacc attttttgca
    33481 gtatgttaat tgaaataaac cattgagtta accctaaatt tattctggac ttataggaat
    33541 agttccaaag atactttaaa atattttctg gaataattgc ttatttcaca tatgttttat
    33601 tgtgatgttt tgatttaata aattcttcaa ctctgtatat agtaaatgta ttatcatgtt
    33661 tgttttattc aagcacactt ataatacgct tgattttttc acaatgatga aatatactaa
    33721 tgggtgttat aggtataaga atagcattac acttgaagaa gtaagtgata aatgagatat
    33781 aaatcctcat atgataaact actatttcat tgaggaacat attctaagtg atgtcaagat
    33841 actacaatat gactaaattc ctaatgataa aaaagtctac gaatgagtgt agaaagggga
    33901 actaagatga ataaagtttt aattatagat gatgataaag atctttgtat tttgttaaaa
    33961 aagagtatct caattgaaca gattgaagca aactattgcc attctgggag agatggactg
    34021 gttgaacttt caaaacaaga ttatcagtta gtagttctag atgtgatgat gccaggtatg
    34081 gatggttttg aaactatgga acaaatacga aaatatagca gtattcccat cttaatgctg
    34141 acatcaaaaa gcgataatta cagcaaagtg catggattgc gagctggtgc agatgactat
    34201 ctaaccaaac catttgaaat tacagaattt actgctcgtg tactgtcttt aatccgacgt
    34261 tacacccgtt ttaatccaaa aaatgaggag ccacagcaac tttctttcaa aggtttgttg
    34321 attgaccttg accagcatac agttactacc aatggcgaac ctatgttgct tccacccaag
    34381 gaatttggtg tacttcttta ctgtgcaagg catcagggta agattttgac aaaacagcag
    34441 atttatgaag ccgtgtgggg tgagccatat gtatatgatg acagcaatat catggctatt
    34501 atcagtcgcc taagaaaaaa aattgaacta gacccatcat ctcccatcta tttgcaaaca
    34561 ataaaaggaa ttgggtatca attcaacacg gaggtatgaa aatggatgaa caaacgataa
    34621 ttcttttctt actcttgctg tcgattggtc tactttttct ttccatattt gctttttcca
    34681 agcttgggca tatttacaag cgcctaaaag atatagagga gatactggct gatgtacaaa
    34741 ttggagacga aaatcgtaaa atactaatta agccatgtga tgttatggct ccattggttt
    34801 atcagcttaa tgagattgtc tacgattatg aaaataagtt actttctctg aaaaagtcgg
    34861 ataaagctag caaacaactt atgacaagtt tatctcatga tgttcgtaca ccattaacaa
    34921 ctctgattgg ttatctggat gctgtacata gtggtatagt tatcggacaa gaacgggagg
    34981 aataccttga aatcgctagg cgtagagcct acgacctcaa ggattacatt gatgttcttt
    35041 ttgactggtt tcggttgaac tctgatgaat ttacgctgtc aatagaatct gtggaaattg
    35101 cagagttgtc acgcaatatt ttgaaagact ggattcctat attccatgaa aagaaactga
    35161 attttgaaat tgatattcca gaaaatcgac taatggtaaa cctcgaccca gatggatatt
    35221 cccgtgtggt caataatctg gttcaaaatg ttcttgctca cagtaaggct cggcaaatca
    35281 aaattaccat gagtgaggat tccagaatgg tattactccg tgttgaggat gatggtgttg
    35341 ggattgccag agaaaattta ccacatattt ttgagcgact gtataagtgt gataaaggac
    35401 gttcagaaaa aggaagtggt cttggtctat ctattgtcag tcaaatggtg gagcgaatgg
    35461 gtggtcagat ttccgtagaa agtgagattg gaaagtatac catatttaca gtgagcttac
    35521 cattaatata aaattgtttt tcccccatgt gacatgtata cacaggggga ttttttgtaa
    35581 gttataaagt tgcaaggtta atacaaggtt gttgcaaggt taatgtaaga ttgaggtggt
    35641 atgataactt taataaacaa aggaggcttt tattatttat gaataattta attattgaaa
    35701 caaaaaatct cactaagcaa tatggtgctc aaaagagcgt ttctaatttg aatattcacg
    35761 ttcagaaagg tcgcatttac ggattgctag gaagaaacgg tgcgggtaag acgacaacta
    35821 tgaaaatgtt attgggtcta acaaaaccaa ctactggaga aattaaaata tttggtcagg
    35881 acattcacaa taacgagaag aagcttcttc ctcgaattgg atgtttgatt gaatcaccag
    35941 ggttttatcc taatctgaca ggaacagaaa atctcaaaat ctttgcaagg cttagaggaa
    36001 taccaaaaaa aaatgcagta gaatcagcac tggatgtagt cggtttgcca aacaatgaca
    36061 aaaagttatt ttctcaatac tctcttggta tgaaacagag gttggcgatt gctcttgcca
    36121 ttatgcatga cccacaactg ttgattttgg acgagcctat caatggactt gatcctattg
    36181 gtattgcaga agtaagaacg tttattcgtg atctctgcac agaacgaggt aaaactattt
    36241 tgatttccag tcacattctt tctgaaattg ccttgttggc agatgacatt ggcattattg
    36301 accatggtat tttgttggag gaagaaagtc ttgcccaatt agaagaaaaa aacagtaaat
    36361 acatacattt tgctgtatct gatgcagcac aggcagcaag gcttctggaa acccaactta
    36421 acctgaaaaa tttcaaagta gcagataatt acaatattat gctatatgat acagatgttt
    36481 ctgttgcaga aataaatcgt gctttcattc taaacaacat tgaggtatca gaatctcatt
    36541 tatgcgaaga tactcttgaa gattacttca agaaagtaac agggggtgaa ggaattgctt
    36601 gatataattt catgtgaatt ttcaaaactc aagtggttaa aatttatact gatttctatt
    36661 ttaggtgctt gcttatttcc aattcccgca acaattttga ttgcaaagga caacttgcct
    36721 tttgagcaat tgttcaaatt agttgtcaat tttggatatt ttttgctatt accaattgta
    36781 ttaagtattg ttgctagcca actatttttt atagaaagag ataatgatgt attaaaaaat
    36841 cttgcaacag tgccagtgca aaaaggaaaa ctggctctgg caaaattggc tgtgttgctg
    36901 tttatatcat tgttctactc tgttgcaggg ttaggagcta ctatcattgg aggtttcatt
    36961 gttggaatag tagagggggt tgctgtaaaa ttaggaatga gcattgcttt tggaattatg
    37021 ttttttgtta ctgtacttcc tgttgtggta ctcattgtat atttcaatcg cagctatatt
    37081 ttttctatta tactttcttt tgtatatgct attttcaatt ttagtatatc cttaaatatt
    37141 atcaattttg aaccaaataa cccaatatta agtgtattac ctgctcctgt tataatgcgt
    37201 tggtggatgg cattttgggg agatcccact ggcgaataca cggcacttag acagccatat
    37261 ttgctctcaa ctcctgcttg tgtaggtatt ttatgtttga taaccattat agcagtcctg
    37321 ctgatttgta tgatatacaa aaagcaggag aattaggagg tatctatgct tgatataatt
    37381 actacagaat ttcaaaaaat aaaaagatat aatattctat ggattggtgt tgttgctgtt
    37441 ctgttttcag cactattggc agtttttcag gttgtaagtt cacacggcag tgaccctcta
    37501 acgtatgaga gctttgcaaa cggagttatc tggaataatt tcagtcttag ttttccattt
    37561 gggattacac ttattggagg atatcttatt aaccgtgaat atacagacca aaccttaaaa
    37621 aacatcctaa ctgttcctat ttcattacgc aaattgctag tgggaaaatt aattgcggtt
    37681 ggaggaatta ctgtattgtt aagtcttttc agcttcttat gcacatttat tttggggttg
    37741 ttattttgca atatcaatat atctgttaca ctcgttgtaa aatccttagt tcagattttg
    37801 tctgttaatc tgtactgtta tgttgctgtt ttgccgatta ttgcttattt tggtcgtaaa
    37861 caaaatgcat ttctgacggg tgtagggatt gcatttgtat acggtttttg tggtgtgttt
    37921 gttgctggaa gaaatctgac agacttttat ccaatcacag caggtcttgg tctagttgga
    37981 tacaacaatg gtgctggtac tgtttatcag ccacttatcg gtgctatgac tttgattgta
    38041 attctaatct tgacaactat tctacttgta tttacaccta attatgataa agttatgacc
    38101 atatctaaga aaaaagggat aaagcataaa aagtattctc ataaataaca taaatattgt
    38161 ttctattatt taagttgcta gacagtgaaa agaaaaaatc tgtcgtacag tggctatatg
    38221 ctcatgtatt caagaagcta cagttttgat ttttcataac tgtagcttct ttttgtattt
    38281 attaaatcac ggctatcata atacttgcaa taggtatagt aacaagagag agtactgtca
    38341 tatgtgtttt atatattaca ttaagtatat tctaaaatat atgttttaaa taatatattt
    38401 gaaaatagga atatttaaat aaaaaaaata atattatata tagttgacaa aaattggcaa
    38461 atgatataat atataaatat agaaatagta atcattacta ttattaaaaa gggaggaatt
    38521 aattatgaac ttaaaaggaa ctaaaacaga aaagaattta atggcaggat ttgcaggaga
    38581 gtcagaagca agaaataaat atacatacta cgcttcaaaa gcaagaaaag aaggttataa
    38641 tcaaatagct gctatatttg aagaaactgc taacaatgaa aaggaacatg ctaaattatg
    38701 gtttaaactt ttacatgatg gtatgccttc aacagaggaa aatttaaagg atgcagcagc
    38761 aggtgaaaac tatgaatgga ctgatatgta tgcaaaattt gctaaagaag ctagagaaga
    38821 aggatttgac aagatagctt atttatttga agctgttgga aaaatagaga aagagcatga
    38881 agaaagatac ttaaagcttt tagaaaactt aaatgaagga aaaatattta aaagagatga
    38941 agaagttgtt tggcaatgtc aaaactgtgg tcatgtgtat gtaggtactg aagcaccaga
    39001 aaaatgccca gtatgtgacc atccaaaagc atattttaat ataaaagctg aaaattatta
    39061 aaaattatca ttattaaaaa ggaatgttaa ctatgaaatt ttctaaacaa cgagaactga
    39121 ttttaaatga aatattaaat aatccggttc atcctactgc ggattaccta tatgaaaact
    39181 taaaaaaaga taatccaaat ttaagcttag gaactgtgta tagaaattta gctcagctaa
    39241 cggaacatgg ctttataaga aaggttagta ttccagggta tccagataga tttgatggtc
    39301 gaatagataa tcactatcat atcatatgtg aagtatgtgg agaggtatac gatttagaat
    39361 ccgaagttct taataattta caagaattaa tatctgagga gacagacata aaaataacat
    39421 cttataacat aagttttaaa gggatttgta ataattgtaa aaggtgtagc caagtaggtt
    39481 agttttcaaa ttctaaaaat aattaatgag gtgagtttat gtgtagtgaa caaaaatttt
    39541 ttatatgtga gacttgtgga aaccttgtag gaatgataca aagtggtggt gtacctattt
    39601 tctgttgtgg aaaacctatg aaggaacttg ttcctaatac aacagatgca gcagttgaaa
    39661 aacatgtacc tgtaattgaa gtggatggaa ataatgttac tgttaaagta agtagcacaa
    39721 ctcatccaat gactaaagaa catcatatag catgggtgta tcttatgact gaacaaggtg
    39781 gtcaacgcaa atgtctagca gtagatggag aacctgtagt taaatttgct ttgaatgatg
    39841 atgataaagt gatttctgcc tatgcttact gtaatttaca tggattatgg aaggctgaac
    39901 tgtaatattt aaataaattg agtcaaaaag gaggctataa atgtattttc atacaaatag
    39961 cctcctataa atttatataa attcaagttt taattttgtt taatgtcaat ataatataag
    40021 gagaaaataa tatgtcaatt tataaatgta gtgtttgtgg atatatttat gatgaatcta
    40081 agaatgataa aacatgggat gaattaagtg aagattggga atgccctgta tgtacaaaag
    40141 gtcgtagtta ttttggaaag ataagtactg tttactatga agaagatgaa aagatagcag
    40201 aagacatagt tgaagatgaa tctaaattaa atactgaaaa agagggagat ttaaattact
    40261 taagtacata tttaagaaga gatgatgaag ttgagaaaca tatggatata attcacgaaa
    40321 tggctgttac aggaaaatct ataatagaac ctatgagaac taaactacca gtaatttctt
    40381 gggatgatat tttaattatg ggtgctcaat taaatccatt acctttaaat gagcatgatg
    40441 aagtaaatac aacaacaata ataggaaaaa aagcaaagaa acctatgatt atagaaaatc
    40501 cagtttatat ttcccacatg tcatttggag ctttgtctaa agaactaaaa atagctttag
    40561 caaagggtgc agcccaaaat aagacagcta tgtgtagtgg tgaaggaggt atattaccag
    40621 aagagaagga agcttcatac aagtatattt ttgagtatgt accaaataaa tatagtgtaa
    40681 ctgaagaaaa tcttaaaaat tctgatgcta tagagataaa aattggtcaa ggaacaaaac
    40741 ctggtatggg aggtcatctt cctggcgaaa aagtaactga agaaatagca aaagtaagaa
    40801 ataagcctgt aggtcaagat gtaataagtc catcttgttt tgaggaaata caatccaaag
    40861 aggatttaaa gaaacttgta gatgaactta gagaagtttc agaagggcgt ccaataggtg
    40921 ttaaaatttc agcaggtcat attgaaaaag atatggaatt tatagcttat gctaaacctg
    40981 attttgtaac tattgatggt cgtggtggag ctactggagc aagtccaaag ttgcttaaag
    41041 atgcaacttc aattccaaca atatttgctc tttatcgtgc tagaaaatac atagatacac
    41101 atggattaga tatagatttg gttataacag gtgggcttcg tatatctact gattttgcta
    41161 aggcaattgc tatgggggct gatgcagttg ctattgcaag ttctgctctt atggcagcgg
    41221 cgtgtcaaca atatcgtata tgtggttctg gtaagtgtcc tgtaggagta gctactcagg
    41281 atgaggaatt gagaaaaaga ttacatatag aaaatagtgc taatagagtg gcaaactttt
    41341 taaatgtaag tttagaagaa cttaagactt ttgctagaat ttctggtcat aaagatatac
    41401 atgatttaag tgttgatgat ttatatactg ttaactctga aatatcaaat tatacaaaca
    41461 tacagcatgt atagattgaa gtttgaaact attagatttt agatagctat aaaagtaaat
    41521 gataaatata ttttataaaa gagagtgtct taaaatgaac tttttagttc ataagtcact
    41581 cttttttgta tgaagccaaa tatatttatc attttagcaa tgaaaaagaa tcttatctct
    41641 ttattttgag atagactcct tctatttatg aaaattaaag atattaaatt gtaaaatatt
    41701 ctgtgatgtc gtactattaa actaaagatt ctgtacttta aagtaaataa aaatttaagg
    41761 agtgattatt atggaaagat tgaagataca agttttagtt gacaataata cttatattga
    41821 tagatacttt gttggagaac cagcagtaag ctattatatt gagattgatg gaaataggat
    41881 tttgtttgac tcagggtatt cagatgtatt catatcaaac gctgaaaaat taaatataga
    41941 tttaggaaat ttaactcatg ttgttttttc tcatgggcat aatgaccata caagaggtat
    42001 ccagttttta gaaaataggt atgatttatc tacagttgaa cttatttcac atcctaactg
    42061 ttttatacct agaaaacatg gagagaaaag cataggagct ccattttctg ctgaggaaat
    42121 taaaaatatt tttatgtata atcctaagga taagccattt aatctaagca agaattgtgt
    42181 gtttttaggt gaaatacctt ctataaatga ttttgaaaaa agagctaaaa ttggtaaatg
    42241 taaaatagga gatttgtggg aagatgatta tgttttagat gattctgcta tagtttgtaa
    42301 aaccgataaa ggtattttta ttgttacagg atgttctcat agtggaattt gtaatatagt
    42361 agagtatgct aaaaaggtat gtggagatga tagagttatt ggtatactag gtggttttca
    42421 tctttttgaa ttgaataata ggttagatag tacaatacaa tatttggata aagaagaaat
    42481 gagaatgttg tacccttgtc actgtgtttc ttttaaggta aaagcgaaga tgaatgaaat
    42541 attgaatata aatgaagttg gagtagggct gaccataagt atatagtatt tgaaagtata
    42601 taagatttaa gttgaaggtt aaaaagatat tttctatata aaatatcttt tttgttacgt
    42661 ttattttaag agaaatcaat atacttttat atgaaatatt gtaaaaaaat ttatttttaa
//
LOCUS       DAEMHZ010000017        40634 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000017 DAEMHZ010000000
VERSION     DAEMHZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.017"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(106..1392)
                     /locus_tag="KPK72_000818"
     CDS             complement(106..1392)
                     /locus_tag="KPK72_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2488062.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(1407..2672)
                     /locus_tag="KPK72_000819"
     CDS             complement(1407..2672)
                     /locus_tag="KPK72_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2488063.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2988..4085
                     /gene="ychF"
                     /locus_tag="KPK72_000820"
     CDS             2988..4085
                     /gene="ychF"
                     /locus_tag="KPK72_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG2488064.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(4564..6843)
                     /locus_tag="KPK72_000821"
     CDS             complement(4564..6843)
                     /locus_tag="KPK72_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2488065.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNIISILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEVEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(7114..8568)
                     /locus_tag="KPK72_000822"
     CDS             complement(7114..8568)
                     /locus_tag="KPK72_000822"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG2488066.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPKAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            8732..9601
                     /locus_tag="KPK72_000823"
     CDS             8732..9601
                     /locus_tag="KPK72_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488067.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(9814..11886)
                     /locus_tag="KPK72_000824"
     CDS             complement(9814..11886)
                     /locus_tag="KPK72_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2488068.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMIDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            12213..15245
                     /locus_tag="KPK72_000825"
     CDS             12213..15245
                     /locus_tag="KPK72_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2488069.1"
                     /translation="MKKTLKRLCTGFLALATVVTALPTMAVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDSHSGISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDIAEVTAGTFYMKELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKVDMEAQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTPHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLEGAYMQAGDKSEQIKGLYLTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSADLLPYGTFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTDENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRSESNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLEKGTKYQLK
                     GWQMLKEENAELIINGKRVENVYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKEVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSENMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFKEKPEVPEKPQTPDTPHKTD
                     SPKTGDSTNLMAFIVMLLASAGGLAGTYLYKRRKMKKS"
     gene            15484..15810
                     /locus_tag="KPK72_000826"
     CDS             15484..15810
                     /locus_tag="KPK72_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2488070.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLIADDI
                     VAVEEK"
     gene            15826..16209
                     /locus_tag="KPK72_000827"
     CDS             15826..16209
                     /locus_tag="KPK72_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2488071.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAIVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNHIPQGQPKK"
     gene            16318..16731
                     /locus_tag="KPK72_000828"
     CDS             16318..16731
                     /locus_tag="KPK72_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 70 family protein"
                     /protein_id="HBG2488072.1"
                     /translation="MVGFKVKYYWFQIGSEDFLFSFFSTVAYNLENQKWGTKFPVIMN
                     ELYQGELKSTSIPMAIKELGSIKQGLANLPPDKIIWDIENLFAKPPWETSISSDITNL
                     SNYFVTSDGENFITVFNHALEKANELNCGVAITII"
     gene            16796..17539
                     /locus_tag="KPK72_000829"
     CDS             16796..17539
                     /locus_tag="KPK72_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488073.1"
                     /translation="MRYKKIGLGVIASVLCLGLLNGCSGYSHDFNSSEEAQKYVSSKL
                     EDKYNEEFTITEVKKYKEEKIGLNWIMVKVSSKEKPSQTATVYARNTGLFEDSYHVYY
                     YSDEIKELANPLFQDKSFIRDYQLEVQGHTTTTKWNGKESEEEYLKKKEYEIESHIYL
                     NEGKTDEEYAEEISCIMQEIIESDLVFNISVYTNDDDLIFYSLPEQHSQPDVEVILEK
                     IADVRRQQETQKNYKEWKKQNQKNENNQD"
     gene            17620..18183
                     /locus_tag="KPK72_000830"
     CDS             17620..18183
                     /locus_tag="KPK72_000830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488074.1"
                     /translation="MDTEVFKLLFCVAAFGWIVYMAFKEKLHKQDPKAFKFLALIFVG
                     LLLAFICFIIPGGAKYTFVLVGVTMGGMTTKIFYQNIKSAIICNEAVNGIYQGYSSYH
                     SGTVSSYAPIFKYKYNGQTYVRQCSNTYSFKKLDKEMVKGNEYQIYINPQIPSSFVLN
                     KRVGIKNVLCLMFGIMCIFIGLSALFV"
     gene            18288..18683
                     /locus_tag="KPK72_000831"
     CDS             18288..18683
                     /locus_tag="KPK72_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488075.1"
                     /translation="MLKEQDIKQYLNEKNIVFKENTSIIGVIFPSKFTYALGPVATAL
                     SMQYFAMNFNDNGIAIIGLNNTTGKLENEAFLFVPKEEITSVKFNKKLMSFELEIITS
                     QGTLAFKVNKTMVGASWHKENLATLISNL"
     gene            18773..20155
                     /locus_tag="KPK72_000832"
     CDS             18773..20155
                     /locus_tag="KPK72_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2488076.1"
                     /translation="MRMIWNKGHRIRASDKHLVYHFSIGSLLFVFVAVLLLLNIKQLM
                     RIDWEHFSLLENSLTLAPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHYGYIEYTLLYDMIANRITIDEVRAENGWLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNVFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAKERQ
                     VMPEQQEKEN"
     gene            20159..20575
                     /locus_tag="KPK72_000833"
     CDS             20159..20575
                     /locus_tag="KPK72_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488077.1"
                     /translation="MEIKEINKILQPEDKGEKAKSVKCICINYGDFLADCEGFVQKRY
                     HDFKCNPKHQFEKKADTILENAIHEKNFMPDLFLIRLNRKQSACNSQIDFVFELLDKS
                     FLETDPIRKSEISEETLNLCLSADVSFIMVYIGMNR"
     gene            20820..22016
                     /locus_tag="KPK72_000834"
     CDS             20820..22016
                     /locus_tag="KPK72_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2488078.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDT
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNNWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDEGNGTDYMETIEQQAKLTEKHEMIIRQQTTPA
                     KDLVES"
     gene            22029..22163
                     /locus_tag="KPK72_000835"
     CDS             22029..22163
                     /locus_tag="KPK72_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2488079.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYEMKQLKESEDN
                     "
     gene            22164..22385
                     /locus_tag="KPK72_000836"
     CDS             22164..22385
                     /locus_tag="KPK72_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488080.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            22448..22990
                     /locus_tag="KPK72_000837"
     CDS             22448..22990
                     /locus_tag="KPK72_000837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488081.1"
                     /translation="MMRLDLRDLHIDILKHNMTYDVFDYSHNGIQFNILFDVNCIPFK
                     LIFIKKKSGQYLILDVLAGYQLNTYLDEKLKILKDMLELKNGKSKFSTNQFFEEFNKK
                     IPHTIQNKKLSKATISHVYHCEESEKIYIRELRNWDKYPELGKHATKENKEKTRLLYP
                     DIYEEIKDKNISVFYTDKEK"
     gene            23057..23353
                     /locus_tag="KPK72_000838"
     CDS             23057..23353
                     /locus_tag="KPK72_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488082.1"
                     /translation="MSDIFKDMQEKVGCEYISDLPSYKRKVWHEMKRLNPADYEESQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQHHTGLTCR"
     gene            23272..23775
                     /locus_tag="KPK72_000839"
     CDS             23272..23775
                     /locus_tag="KPK72_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2488083.1"
                     /translation="MQETRVLVETRGTTGEETTSYWFDLPIDVAEFEEKLSVGAESGE
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            23793..24296
                     /locus_tag="KPK72_000840"
     CDS             23793..24296
                     /locus_tag="KPK72_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2488084.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNDMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            24415..24813
                     /locus_tag="KPK72_000841"
     CDS             24415..24813
                     /locus_tag="KPK72_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488085.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            24791..27241
                     /locus_tag="KPK72_000842"
     CDS             24791..27241
                     /locus_tag="KPK72_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2488086.1"
                     /translation="MFPIKYIDNNFVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNMKKSAWLTFTEFLHEVNHTLMNDFVSMPSDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGKALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPVSRNIADHIESFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            27241..29460
                     /locus_tag="KPK72_000843"
     CDS             27241..29460
                     /locus_tag="KPK72_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2488087.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVVLSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTSNGFSSSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYIKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMERRMARAVSHGSMVGAGTGAVTGAMLSGNGSSRSNPQKDKRTFSRSN
                     TSSSFGNRAGSAVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSD
                     FKRGMVQEQQSRQTGRLEKQEQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRP
                     HERPATASIIPKPSAEKTQEVKRPATATTSKASEPVKTNVIKERPLSSGASDVKATQS
                     VQPAHRQNVEKVVSQETRQNYTKDRRTKVQQTQNVQKNQQTTEKTRNLVTKKGQKKK"
     gene            29457..30464
                     /locus_tag="KPK72_000844"
     CDS             29457..30464
                     /locus_tag="KPK72_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2488088.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVTVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTYNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGEAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNKMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            30480..31391
                     /locus_tag="KPK72_000845"
     CDS             30480..31391
                     /locus_tag="KPK72_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488089.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQA
                     ISGYLTKELQDLNVDTIRTDIPTSSTVTDVLVWNIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTANEATA
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYLYSELVNPVFTKDGDNVKVKVAVKF
                     LDNQTKSTLVSQYELVLHKDSNWKIVG"
     gene            complement(31447..31809)
                     /locus_tag="KPK72_000846"
     CDS             complement(31447..31809)
                     /locus_tag="KPK72_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488090.1"
                     /translation="MEIDYKEIGQRIKIARIKKGITQEAVADLIDITPAHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVIASKIVFEKEAKDIFSDCDEYEVRFLVDLMK
                     SAKIAFRKDKDIRNQFQK"
     gene            31960..32184
                     /locus_tag="KPK72_000847"
     CDS             31960..32184
                     /locus_tag="KPK72_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488091.1"
                     /translation="MLRFKKKDSKELLKRVAKIHGCSVSEIREEMQYAIEEARNNDDP
                     DKQAEFQRLFGNRTPSPEEFIYTISKKLKV"
     gene            32204..32566
                     /locus_tag="KPK72_000848"
     CDS             32204..32566
                     /locus_tag="KPK72_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stathmin-1-A"
                     /protein_id="HBG2488092.1"
                     /translation="MKKAEMQEQEFLSVCSSYDYDLLTGKSEMTLKDYERITYLVSSL
                     GYSQYLQFLIGKYMDLAQADEEREEREHEIYLEYPEYYEDEGILEEEEKWLEEFLNQL
                     PENKREYFERLIKENQSY"
     gene            32748..33428
                     /locus_tag="KPK72_000849"
     CDS             32748..33428
                     /locus_tag="KPK72_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488093.1"
                     /translation="MEEKYKILIVDDEQSILDMLKLQLEFEGYSVYIANNAKEALDKL
                     SYLPDIILLDINMAGMNGLDLCVSIRDFISCPIIFLTARVSEQDKINGLMAGGDDYIT
                     KPFSVNELLARISAHLRREQRSHNKTKSKFSEELIIDYSDRSLYIKGNKIELSNKEFE
                     IIQLLSSNAGQVFDREKIYECIWGIDGNGDSVVIKEHIRKIRLKFSEFTDKVYITTVW
                     GVGYKWEK"
     gene            33416..34633
                     /locus_tag="KPK72_000850"
     CDS             33416..34633
                     /locus_tag="KPK72_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488094.1"
                     /translation="MGKIKKTFQKMSLKKSLLVLSVFYLSLVSVLSIVTILKCSNIRQ
                     EILDTRPIIITDYIIDDTNINGAENNNGVTAVPKKYTHGELTKENQVYYWCITILMVS
                     LPIAYIILASFMVAKLYYKWKLQIPLEHLKNGMYHISQQDLDFQIPYTSNDELGQLCD
                     TFEHMKNEIYRSNRKMWDMLQERKALTASVSHDLRTPITVINGYLDYLEKSIERETLT
                     SEVLQATIENITGATERLERYVDCVKDIQKMEDVEIKMDNYDLKELIAGITREFSVLA
                     TQQSKQFSVHDLSKTAFIQTDKDMLLKILENIFDNALRFSSEKIMCLIEEKEEYLYFS
                     IQDDGVGFTSEELKSATSFFFSSSTNGGNFGIGLSICKILCEKLGGDMCLDNTAEHGA
                     IITIRIKNNRKLF"
     gene            34689..35564
                     /locus_tag="KPK72_000851"
     CDS             34689..35564
                     /locus_tag="KPK72_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488095.1"
                     /translation="MEIKIEHLNKNYGKQRALKDISLHIPVGMYGLLGENGAGKTTLM
                     RILATLLEQSTGTVEINGIDIKRKKEIRKIIGYLPQEFSVYPNMTVYSALDYLGILAE
                     LPKDIRKSRVSELLKQVNLEQEKNKRFKNLSGGMKRRFGIAQALLSNPQILIIDEPTA
                     GLDPEERLRFYNLLSELAMDRIILLSTHIASDIEATCSNVAVLSSGTVIFEGQVKNLL
                     QLGYRKVYTTTIMQNELSQFKKKHHVISIHQNGTDVSCRFLSNTSPQKEWVSCQPTIE
                     DSYMYLLSSFKQGVE"
     gene            35565..36959
                     /locus_tag="KPK72_000852"
                     /pseudo
     CDS             35565..36959
                     /locus_tag="KPK72_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            36892..37539
                     /locus_tag="KPK72_000853"
     CDS             36892..37539
                     /locus_tag="KPK72_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488096.1"
                     /translation="MDTSCKNILEFLKIDFWLVFKNSLLLAVAYTFLIPVIRGISNLD
                     AAHSADVFGQSLALIGVFLFVPITSQELESSIKEIVYTKVWSYKKSVSLRLICGFLLS
                     TVIVTIFAGIMQLKNCDFPFLEYISVTILYAVFLGLLGLLFSQVGNNVIAGYLVSLGY
                     WSFCQLHILAEENLMYLFPIINGEIDMEKLLILVIVNICLLVLFFWIVKRTKGKV"
     gene            37803..38222
                     /locus_tag="KPK72_000854"
     CDS             37803..38222
                     /locus_tag="KPK72_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2488097.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEREIPFC
                     ELPEIVVEKLAVWDEYDSDYTLFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKKLKEKR"
     gene            38226..38474
                     /locus_tag="KPK72_000855"
     CDS             38226..38474
                     /locus_tag="KPK72_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488098.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIGKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            38850..39053
                     /locus_tag="KPK72_000856"
     CDS             38850..39053
                     /locus_tag="KPK72_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2488099.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            39132..40322
                     /locus_tag="KPK72_000857"
     CDS             39132..40322
                     /locus_tag="KPK72_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2488100.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEESLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILENRGKAQPLIVGGYSNFLFLNRGGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
BASE COUNT        13651 a         6828 c         7510 g        12645 t
ORIGIN      
        1 ttacctcaat ttatataaat atattttata aatttgtttt accaaaataa acatttaaaa
       61 acataaacta atacttaatt agttattctt attatgatta agtttctatt taggctctac
      121 aatagaaata acacaatact cttctttaaa atgctctttt aatctttctt caacttcctc
      181 aaaagtaata tctttaataa tatctaaata atccaataga tttatatcct taaaaacata
      241 agatataaaa ctattaccta taaagtttat agaatcaaag cacttaataa atgaccctat
      301 ttttttcttt ttagttctct caaactcttc ttttgataat ccttcttttt ttgatttttc
      361 tatataatca agaattattt cttttacttt tttaggctct ttagaatctc cagctattat
      421 gctaaaagca taatccactt gagacgaaaa tccagctcca aaattttcat ttattaaacc
      481 ttgcatatac aaatcttcat aaagcttact tcctttttta aataacatcc ctacaagtat
      541 atcagttaca atctctttac gtaaaagttc tttccctttt aaaccaacat tgctatcttt
      601 aaaacctatg ttgaacatag gcatagatat agggaatttt tctataacct ctttttcttt
      661 tacaccctct ggttcttctg gataaaatct ttctatactc ttagagagtt tgtctacttt
      721 gtaattgttt gactttttag ttacatctat aactttttct acatctaagt caccaacaac
      781 aaataaagcc atatttcctg gattatagaa agtattataa catttatata gctcttcctt
      841 agttatctta tatatactat caacagtacc tgctatatca attctagcag gataatttac
      901 atacattgcc ttcaagcaat taaagtatac attccaatca gggtcatcat tgtacatttt
      961 tatctcttgt gctattatac ctttttcctt ttcaacattt tcatctgtaa aataaggtgt
     1021 ttgaacatag tcaattagat gttctaaact ctcataaaaa ttttcagtag cagaaaataa
     1081 gtaggctgtc attgtaaaat tagtaaatgc atttgcattt actcctaatt ttgaaaattt
     1141 atcaaatgca tctcctccat caggttgctc aaacatctta tgctctagaa aatgtgcaat
     1201 accttcattc actctaattt tcttatcttc tccaattggt acaaattcta agtcatttga
     1261 accataatta gtagccaaaa tagcatactt tttcataaaa ccccttttag gcataaaata
     1321 tacatctaat ccattttgca atttctcata ataaacttct tctttcaata tatcattaac
     1381 tattttttcc atagacatct cctccattaa tttctcaaga agtatacagt atcaagctct
     1441 atatttttta tagcttcaac tatatctttt tttgtaacct tttctatact agatattata
     1501 tcttgtactt cagaatttgt ctttgccata gattgagaga aaacaaaatc agacatacca
     1561 cctatattat cagttataga tttcatagaa ttaactaaag caagtttact gttttctaat
     1621 tcttcatcag atatatcacc tgcttttaca ctttctactt gtttttttat caattctaca
     1681 gctttatcat aatctttagt ttctatacca gatgatataa acatgatact tttatacttc
     1741 tctattgaag agaatatata gtaacataaa ctttcttttt ctcttacatt aacaaataat
     1801 ttagagtgtg gaccaccacc taagacattg ctcccaacta ctaaagcata atatttatct
     1861 atgtctgcat aatccacatt tgctctataa cccataacta acttaccttg agttatttcc
     1921 atttgttcat ctataacttt tacctcatcc acatttttta taaaatctgc tcttggtata
     1981 tttataattt cttctctttc aaattttaga tttttactta ttatatctac aactttatct
     2041 tcatcaaaat taccttctac aactatatct ataggtgatg ttttaagtat gtttttataa
     2101 tgttcatata actctcttga agttatacta tctatttcat cttcataacc aaattcactt
     2161 acactatatc tctctccttt acacatagcc tcaatacatt tatcttgcgc atattccttt
     2221 ttatcattta ttattgattg tatcttttct cttaaattct ctttttcaat atttaaatat
     2281 tcttctttaa acccaccatc tacaacaagc gtattattta ttacttcatt aaaaaattct
     2341 actacttctt caaatatact ctcatctaaa tacttttcac ttatgttaat tactttaaaa
     2401 ctcaatactt gacgttctcc acgttttaca gcatcagctc ccatagatga accatataaa
     2461 tcatctaatt tattggatat tgctctagca ctaggatatt tagcgcttcc acttgctatt
     2521 attgatggta ttagagcatt ttttgtagtt tctttttcat ccaatagtct ctgaacatat
     2581 aaacttataa gattcgattt aaacttttct gtttttatca aagtaagatt aatattatta
     2641 cctaaattta ttattttttt agtattaacc atatgtacct cctattttta tttaaacatc
     2701 aattttaaaa ttgaaattta tttatttttt aattacatat actcacatat aaaaaccttc
     2761 tatttaatta tatccttttt attattttct aaaagcaata tttttattta aattaaatac
     2821 aaatattatt taaatcaata tgctttaaag tctagtttac aagcttgata tatatgatgt
     2881 ataatcaata tgtatgtcta taattaaaac aattttgcaa tcctatagat tgattgttta
     2941 aagttatata aagaatataa aaaagaataa gagagaggac gattaatatg aaattaggta
     3001 tagttggttt accaaatgta ggtaaaagca cactatttaa cgcaataact caagcaggtg
     3061 cagaatctgc aaattaccct ttttgtacaa tagacccaaa tgtgggagtt gtatctgttc
     3121 cagatgaaag attaaacaaa ctacaagaat tatataattc tgaaaaaata gttcctactg
     3181 ctatagaatt ttgtgatata gcaggtttag ttagaggagc ttctaaagga gaaggtttag
     3241 gaaataaatt cttatcacat ataagagaag ttgatgcaat agttcatgtt gttagatgtt
     3301 ttgaggatga aaatgttgta catgtagatg gttctgtaga cccacttcgt gatatagaaa
     3361 ctataaactt agaattaatt ttctcagata tagagattct tgaaagaaga attaataaaa
     3421 ctcaaaaagc tgctaaggct gataaaactt taggttctga acttgattta ttaaaatcaa
     3481 taatgtctac tttagaagaa agtaagtgtg taagaactat ggaatttaca gaagatgaac
     3541 aaacatttgt aaattcattg gatttactta catctaagcc tgtaatatat gcttcaaatg
     3601 tatcagaaga tgatttagct gataatggag aaaataataa atatgttcaa caagttaaag
     3661 catttgcaga aacagaagat gccgaagttg ttgtagtatg tgcacaaata gaagcagaaa
     3721 tttctgaact tgattctgct gaagaaaaga aagagttttt agaaacatta ggtcttgaac
     3781 aatcagggct tgataagctt ataaaatctt cttatgcttt acttggttta atttcattct
     3841 taactgctgg tccaaaagaa gttagagcat ggactataaa agttggaagt aaagctcctc
     3901 aggcaggagg aaaaatacat tctgatatag aaagaggatt tatcagagca gaaacaatag
     3961 cctttaatga tttggttgag catggtacta tggctgctgc taaagaaaaa gggttggtta
     4021 aattagaagg taaagaatac atagttaaag atggagatgt aatattattt agatttaatg
     4081 tttaaaatga ttaatcgtat acactttgac tattatatat aataactaaa aaactaagat
     4141 tataataata caatattatc ttataataat tatatagaat ttaaaaataa aagaagagta
     4201 tttcaaatga tttgtataaa ttattatgaa aaactctttt tttgatataa taaaattact
     4261 tacatatata acatttaaat ttataaatta tatttttatt ttttaaaata tgtataacac
     4321 acctatagaa attactatta ttactatcta aagtattttg ctataatccc atatactctt
     4381 acctcaaaaa cattcaaatg taaaaaatac tacatattaa taaactttgc ccaataattc
     4441 ctaaaataat atcctaacaa tgatttatgt ttttataata gtatattttt acataaaaaa
     4501 gtgaacattg attaacaaag ttcacttttt tataaatttt tatgttataa ttcacaattt
     4561 tatttaattt gtttcttttc tatttaaact atatctatct aatattaatt tttcatattc
     4621 ttctattggc attggtctag caaataaaaa tccttgggcc atatcacaac ctataaacct
     4681 taaaaactct gcttgctcta cagtttcaac accttctgaa acaactttta tatcaagttg
     4741 ctttgccatt tgaacaatac ttgaaactac aatttgactc ttttttgcat ttcccactgt
     4801 tataaagaat tgcctatcta atttcaatac atcaattggc atatctttaa gcatatttaa
     4861 agaagaataa ccagaaccaa aatcatccat agaaatttta aatccaacag atttcaaagt
     4921 ttgcattatt ttaaataata tttttgtatt gtcaaatatt gcactttctg tcaattcaat
     4981 ctcaattaaa tttggttcaa cttcatattc ttttgttatt ctttcaagct cagaaataaa
     5041 gcctggattc tcaagatgta ctcttgataa gtttattgat ataggaaatg tggggaaatt
     5101 ttcattttcc caacgtttaa aatttttaca aacttgggta aatacaaaca tatccaattc
     5161 tgttataaag ccatttttct caaaaatagg aataaataca cctggactta ttagaccttt
     5221 tttaggatgt tgccaacgta caagtgcttc agcacctaca attttagtag ttgataaatc
     5281 aaccttaggc tgtaaataaa ctttaaattg ttcatttttt aaagcatcat gcatttcttg
     5341 ttcaatatca cgttcttcta tgagagtttc ttttaaattt tcagcataaa tctcataaac
     5401 ttttaaatat tttcttttta ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga
     5461 aatatttgtt tctccctctt caacaaaata tacacctatg gatggaatta aattgagttc
     5521 tactcccaaa cttgttttta tcattgacaa ttttttacgt ataagttcac atatttctat
     5581 aattgactct ctattatttt tcttttcaaa tattatagca aaattgtcat ttgaaattct
     5641 tgaaaataca gattgctctt taaatatatt cttaattatc tctgctattt cctttaatac
     5701 tctatctcct tcttcatacc caaacctatc atttactagc ttaaacttat ctatatcaaa
     5761 ataaattaaa actagattct ttttctcatg agaggataaa aattttcccc cttcaagatt
     5821 aaatttattt gaattgccaa tacctgtaac gttatcttta aaagcaaatt cttccaattt
     5881 ttccttactc tttttatgta taaatagtat ataaattgtt aatgccgtaa agattataat
     5941 caagactaca catgctgata atgtaagttt tataatagca tatgatttct cagatatagc
     6001 agttttagga acttcagaaa aaagatacca atcattgatt cctattgggg cataacttaa
     6061 ataatatcct tttccaaaca tactataact taatgaacct gatttaaagt tttttatatt
     6121 tgacttcata ttgttgatac tagattttga tattgaatca cttctgccaa tgtaagaaaa
     6181 taaattttct ttagtagagt tggcgttttt actgcttgga tttaaaacaa tatcaccatt
     6241 acttctagtt atataagaat atccttttcc attaaatgta ggtacagaaa gtattttttc
     6301 atacaacttc gtactatgtg tggcaaatat aactccttta acctcattat ctttataaat
     6361 tggtacagaa tatacattaa tattactatt ggtattttta tcatcttcta ttacatcttt
     6421 taattttcct gttataacca cttctccttg cattgatttt aagaaataat ctcttttaga
     6481 gaagtcttct atataaccat ctgtagtgta tgctgtacca tttggtaaaa taactcccat
     6541 tcttttaaat gaactattaa tagcttgtct ttttagtata gaaatgatat tatctacttc
     6601 aaaagactct tctccttcaa taaatattga aattgactct agtaacttta tatctccatt
     6661 aatttgtttt tttaacacat tgacgctttg atttgctatt tctgaaagat attgattcgt
     6721 ttcttcacct aatgtcttat tcaaccttga accataaatc aaacaggaaa ttgtaattac
     6781 taagatactt atgacagcaa ttatggacaa accataccta ttattttttg catcacttaa
     6841 cattatgctt cctgcctttc tcgaatctat gtatgtataa tcattatctt atgacaatta
     6901 ttgtaaacat tttataataa tacttatttt tccatattta ctgtaattat acaaaattat
     6961 aaactttatt acaacaataa attaattaat atagatatta gtgtttacat tattgcatta
     7021 tctattttac aattatttac atataaaaaa atgtaatatc aaagtgaagc ttgatattac
     7081 atttttgttc ttgtattttg tttaattttt atgctacatc ttacttaaac actcatttgc
     7141 aacttcttta tcatcaaaat gatgcttaac tttacctata atttgataag tttcatgtcc
     7201 cttacctgtt ataattacaa catcatcctt ttttgccata cttatagctt ctgctatagc
     7261 ttgttctcta tcaacaacaa catggtaatt ttcttttttc ttgtcaattc cttcaagtat
     7321 atcttttatt attgcctttg gttcttcagt tcttggatta tcagaagtta taatacataa
     7381 atcagaatat ttttgtccaa ttgctcccat caaaggtctc ttttcagtat ctctatctcc
     7441 accacaccca aacacagaaa ttatttttcc ttctgcaaat tgttgagttg tctttagtac
     7501 attttcaagt gcgtcaggag tatgagcata gtctacaatt acgcttatac ctttatcatt
     7561 tggaactgtt tcaaaccttc ctgatactcc attagaaagt ctaaggcctt ctttatatat
     7621 tggtttaggt atgccaagta catagcaagc agctataact gctaaagtat tatatactgt
     7681 aaacattcct ggaactggta taaatattac ttcttcatat gaaggtgtta ttaatctata
     7741 agatactcca tccgaatctg attttatatc tctagccata aaatcagcct tagtatcaac
     7801 accataagtg taacaaggta catttatacc ttttatattt tcatatatct tttttccacc
     7861 ttcatcatct atgtttatta tatttgccat agtagtctta aaaaataatt tttcttttgc
     7921 ttttctataa tcctctaaat ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa
     7981 tatgcctagt ttaaagtctg tttcatcaac tctatttaat gccaatgaat gtgatgaaac
     8041 ttccatcgca cagtagtcac atccattatc caacatatta ttaaaatgat attgtaaatc
     8101 tatactttct ggagttgttg aattggaaac aatgtctttt tctccatcaa aaatttttat
     8161 agttcctata agtccaactt tattcttatt taaagttaat atctcattta gtatagtagt
     8221 tatactagtt tttccattag tacctgtaac tcctataacg ttaaattttt gagatggatg
     8281 attgtaaaaa ttatcagcta cttttgccat atcttttctt gtatctttta cctttataaa
     8341 tgtacatcct tttattgcta catcttcttc aacaagaaat gcacttgctc ccttttctat
     8401 ggcatctttt atatacttat gaccatctga cacaaatcct tttatacata taaataatgt
     8461 accttttgta acctttcttg aatcatattg tacattatta atatctatat ttaattcacc
     8521 cttgacattg attatatcca acccctgtat aatatcattt aatttcataa atgctttcct
     8581 ccctttaaac tctttctgct aaaattacac tacatataat attatattaa gaatttttta
     8641 ctatgtgaat ggttaaaaat gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa
     8701 tataactgag gaaaggtctt ctataaaaac tatggataat aaaatcgggt acttaaacga
     8761 taactttaaa atatttaata ttagagataa aaaagatata caatttgaat accataatca
     8821 tgattttaat aagataatta tatttataaa tggaaatgta acttattttg ttgatggtat
     8881 tccctataaa ctaaagcctt gggatatact ctttatcagc aataatgaaa tacataaacc
     8941 agttatagat tcaaatgtct tttatgagag aattgcaata tggattagcc cagaatttat
     9001 gaaaaaaaat agtgatgcta gtagtgactt agaaaaatgt tttaaaatag ctacagagaa
     9061 taaatgcaat agattgcgtt taaatatgca tgatattgaa tctattaaga gttttattga
     9121 gcaaataaat atttccgaat ccaatactga atttggaaat gaaatattga gaaatacatt
     9181 atttatacaa cttatgatat tcttaaacag aattttccta aaaaatgaaa atatggaatc
     9241 aacagaagat gttttttatg atgaaactat taagaaagta ttaaactata taaattcaaa
     9301 tatagatgat aagctttcaa tagatagcat atcgtctgaa ttttttataa gtaagtatta
     9361 tcttatgaga aaatttaaat ctcatactgg aacgtctata cacaattata tagttcaaaa
     9421 aagattaatt ttggcaaagt cactaataac caatggtcac cttatgagtg aagtttgtaa
     9481 taaatgtgga ttcaacgatt attctagttt tgtaagggca tttaaaaaaa actacggggt
     9541 atctcctaaa aattatataa acaataaaaa tcctttggat attactcttt tagatgaata
     9601 gataatttat gtaaatttta ttttaataaa acttgaagta ttagtgtatt tatcttacta
     9661 atacctcaag ttttttaatg ttttaaaata tatcaaaata taatgatata tatttctatt
     9721 acttaaaata tatattaaat aaattgcatg ttaactgaac ctatttatat atttattaaa
     9781 tcccacataa cttttagtta cactatcata tagctatcct attttttcaa tatcatctct
     9841 taatatactt actctattct cctcttttgc tgtggttact ccttttttaa tgcttaattt
     9901 ctgattttgt tctaaattcg taaatactac aggacttatt gttgatagac catttttttc
     9961 aatatattct aagtccattt taactaattt ttctcccgct tttacttcat cccctacatt
    10021 cacaaatact tcaaatccat ttccttctaa atttacagtt tctatcccta aatgtataag
    10081 tatttctact ccactttttt gggattttat tattattgca tgcttagttg gaaatataac
    10141 tccaatagtt ccatcaactg gtgaataaat caatccatca ctagatttaa ttgcaaatcc
    10201 atcccccatc agtttacttg aaaatacaga atctggcact tcttctatat ctaatatctc
    10261 tccatttgtt aatgaatcaa tcttttctac taaagcattt tccatatttg caacttccat
    10321 attttttttc actttatttt tatcaacatt tttagtaaca gtactttcaa cctttgtttt
    10381 tccctctatt atatctttta tatcagactt tattgaatcc gattttggtc caaatatagc
    10441 ttgaacattg ttgccaataa ccataacacc tgctgctcca agtgatttca atttttcttt
    10501 atctacttta tttatatcat ttacaaccaa tctaagtctt gttatacacg catctaggct
    10561 ctttaaattg tctttatttc ctaaagcaat caaaatctct gatgctaatt cattcttaga
    10621 catttcaatg acttcagcgt tagtttctga ctcatcttct cttcctggtg tctttaaatt
    10681 aaattttctt atcataaatc taaatacact atagtataat actgcaaaac caatacctac
    10741 caagatagcc aagtaccatg gagttctatt aggaagaaca ccaaataata agaaatctat
    10801 taatcctcct gaaaatgtga atccaatttt aactcctaat aattgcataa ttgcgtatga
    10861 taaacctgaa aataaacaat gtattgcaaa taatactggt gcaacaaata aaaacgcaaa
    10921 ctctattggc tcggttattc ctgttaaaaa agaggttaat gctgctgatg ccattatacc
    10981 tgctacaaat tttttctttt ctggctttgc ttcatgcacc atagcaagag ctgctgctgg
    11041 aagaccaaac atcataaatg gaaatgcacc tgtcataaaa gtacctgctg tgaaatttac
    11101 tccatcttta agttgagcaa agaatatctt ttggtcaccc attacaagct caccagcttt
    11161 atttacatat tctccaaatt gataccaaaa tggattatgg aaaacatgat gcaaaccaaa
    11221 tggtattaat agtctttcca aagaaccttc cataaatgct gctagtgttt gatttgcttc
    11281 tataacattt tttgagaaac taagtaatcc ctcttgtatt ggaggccaaa taaaagacaa
    11341 tataattcct attgctatag caactaatga agtaacgatt ggtacaaatc ttttacctgc
    11401 aaaaaaacct agatactgag gtaattcaat tttataaaac tttttataaa gataagcagc
    11461 cactatccct ataataatac caccaaatac ccctgtttga attgttggta ttccaacaat
    11521 atttgcatac atagaattgt ctatcatatc ttctgtaaca cctgccataa cacccataga
    11581 tgtattgaga attaaaaaac ctactatggc tgccaatgct gcaacacctt ctccttcagt
    11641 taaaccaaca gctaccccaa ctgcaaatat aagtggtaag ttattaaata taatttgacc
    11701 tgaactttcc attatactta ttataagttg aaatgtagga ttcgccaaag ctggaaaatg
    11761 agccaacaat gcgtctcctg ctaaagcatt actcacgcca agtaatatac ctgctgcagg
    11821 aagtaatgca actggtagca ttagagattt tcctactttt tgtaaaacac caaatacttt
    11881 tttcatttac ttttctctcc ttattatttt atattttttt ctatacaaaa acacaataaa
    11941 aatctgatta tccgctaaaa cacagcatct atcagcattt tcaaggaaac aaacacatca
    12001 atttactact tatttactac cattgaatga gccttgatac gcaagttttc ctagatatgc
    12061 aaagatatgg tacagcacat cagtattgaa acaagaaaaa aattgaataa ttcatagact
    12121 atggaacgcc taaaatgacg ttccttttct atttccagcc gttcttattg gatggctatt
    12181 ttattaccca aaatgaaagg agcattagat ttatgaaaaa gacattgaaa cgattgtgta
    12241 cgggcttctt agctcttgcg actgtcgtta ctgctctacc gactatggca gttcatgcag
    12301 aaagtaagca atactggacg gagtcaaagg agcgtgtcgg tattgttgaa aaagtaatga
    12361 atgacggttc gataggctct acctttaatg aggggcattt gaccgttgag ggcgaggacg
    12421 cttactgcat tgacatcaat acagatttta agaacggtta taagaccaga gctgacgcaa
    12481 gctcacgcat gagtgccgac cagataagcg acgttgcctt atctatcgaa tatgtgaaac
    12541 agtacacaga cagccacagc ggaataagca acaatcacgc ttacctttta agacagcttg
    12601 ttgtctggca gagattaagc gtacatcttg gctggcaatg tgataacgtg cgagcttctt
    12661 atgatgaaat accaaaggct acgcaggacg aagttttcgc tggtgcaaaa gcctttgtca
    12721 aagaaaataa aggacgctat gaatgtggcg gttatatcta ctctggcgag gggcaggaat
    12781 taggacaatt ctgggcgaag ctgaatgtcg gaaatgcgaa acttcaaaag acttccagta
    12841 ataccagcat tacagacggt aacggcaatt actctatcgc tggtgcaaca tacggtgtct
    12901 tttctgataa ggactgcaca aaacagcttg ccacccttac gactgatgaa aacggaaata
    12961 cggacattgc agaggtaacg gctggcacat tctatatgaa agaactttct gcaccagcag
    13021 gatataaagt tgatacaacc gtatattcct taaaggttga agctggaaaa acagcaactt
    13081 tgaacgtatc tgacacacca aaagtaacag acactttgat tgagcttttc aaggttgata
    13141 tggaagcaca gaaagacaat ccacaaggga acgcttcttt agcaggtgcg gaatttacat
    13201 ggaagtatta tgcaggcttc tacaataaag acaatcttcc tgccgaagct actcgtacat
    13261 gggttacaaa aacaatcgct gaaacagaca gcgacgggac accccactac atcacaaaat
    13321 tagcggacgc atacaaggta tctggcgaca gcttctatat gcaggacggc aaggctgttc
    13381 ttcctttagg aacactaacc gttgaagaaa cgaaagctcc aaacggctac ttgttagagg
    13441 gtgcatatat gcaggctggc gataagtcgg aacagataaa aggcttatat cttacacaga
    13501 ttaccgagga cggcgacctt gccgtattat ctggaagtaa ccagttttct gtatcagaca
    13561 aagttatccg tggcggtgtg aaaattcaga aacgagattt agaaacgggc gatacgaagc
    13621 cacaaggaag tgcaacattg aaagatactg cttttgacat catttcctta aatgataatg
    13681 cggtattggt tgagggcaaa ctttacaaga aaaatgaagt agtaaagaca atccatacgg
    13741 atattgaggg tgttgcttct acttctgccg accttttacc ttatggaaca ttccgtatcg
    13801 ttgagagtga agctccagac ggttatttga ctgacggtgc gaagcccatt gattttacaa
    13861 tcacagaaaa cggaaaaatc gtggacttaa ccgacgaagc ccattctatc tacaatcaga
    13921 ttaagcgtgg ggatattgag ggcgtgaaaa tcggggcagg cacacacaag cgtcttgctg
    13981 atgttccctt taggatcaca agcaagacga cggacgaaaa tcatgtagtg gtaactgatg
    14041 ataacgggca attctcgact tctgctgact gggcttctca taagcacaat accaacgcag
    14101 gaaagaccag cgaggacggc gtgtggtttg gaacttctga accagatgac agcaagggtg
    14161 cattgcttta tgacacctat atcattgaag aattacgctc tgaaagcaac aaaggctttg
    14221 agcttatccc accttttgaa atcgtggtat caagaaataa ccttgtcatt gatttaggaa
    14281 cgctgactga tgaatacgaa aaagaaatct ctatccatac cacagcgacc agcaaggacg
    14341 gcgaaaagac tattcttgca ggaaaagagg ttacgattgt tgataccgtg aagttggacg
    14401 gattggaaaa aggcacaaaa tatcagctca aaggctggca gatgttaaag gaagaaaacg
    14461 ccgagcttat cattaacggg aaacgtgtag aaaatgttta tacttttgtt gctgatgagg
    14521 aagaaatgaa agtggaaatt gcctatacat tcaatgcgtc tgctttaggt ggcaaaaacc
    14581 ttgttacctt tgaagaattg tatgatttaa gcaatccaga cgaacccgtg aaagttgcgg
    14641 aacataagga cattgaggac gacgggcaaa cagtattgat tacagagcgt atcatcaaaa
    14701 ttcatacgac tgctacggat aaggacggca ataaagagct tgaagctgga aaagaggtaa
    14761 caatcattga taccgtaacc ttagagggct tagaagtcgg cacacagtac aaacttgtag
    14821 gctggcagat gttgaaagaa gaaaatgcag agcttctaat caacggaaaa cgtgtggaaa
    14881 gtgattatac ttttactgcc gacagcgaaa atatgaaagt ggaagttgct tttacttttg
    14941 acgctacttc tcttgacgga aaacagcttg taacttttga agaattatac gatttaagca
    15001 atccagacga gccgaagaaa gttaccgagc ataaggacat tgaggacgag ggacagacga
    15061 ttacctttaa ggaaaagcca gaagtcccag aaaaaccaca gacaccagat acaccgcata
    15121 agacggacag tcccaaaacg ggcgacagta caaaccttat ggcatttatc gtgatgttgc
    15181 ttgcgtctgc tggtggactg gctggaacat atctttacaa acgccgtaaa atgaagaaat
    15241 cataaggcgg taacagtatg aggaaaggaa aatcacgttc tccgccgaag ctgtcaaacg
    15301 gcagacttct gtttattctg gcttgtctgc cctgaacctg caagaacacg gatagtcaag
    15361 ggtgcgtaag cattgagttt accttttact atctgggggc ttgctggtat ttcccgaacc
    15421 gccagaataa gaaatcacaa tcaaaaaact tatcaaaaat agaaagaaac gaggtaaaac
    15481 actatggaaa tgaaatatgt cgtgccagat atggcacagt cttttggaac tcttgaattt
    15541 gcaggcgaaa gcgagccagt ctttgaaaga gataaaaata accgcagggt cttagccaga
    15601 cgaagctata acctttattc tgacgtacag aaaggcgaaa atgttgtcgt ggaaattccc
    15661 gtgcaggctg gcgaaaagca ttttaagtat gaacagaaag taaaacttgt caatccgaag
    15721 ttatacggca ggggttacgc aatcggggat atgggacata ccgattatgt gttaattgct
    15781 gatgatattg tagcagttga agaaaaataa aggagtgaag aatttatgag attatcaaac
    15841 ggttttgtta ttgacaaaga gaaaacattt ggagaattaa aatttacagc ggtgcgtgat
    15901 gtgttcttgc agaatgaaga cgggacaccg agtacccaac ttaaaaagcg tatctatgat
    15961 ttgaagtgca gtctgcatgg tggaattatc cccgtgtccg taccgccaga agtaccgtta
    16021 agagaatttc cgtacaatgc tattgtggag cttgtcaatc ccgtagccga tacggtatcg
    16081 agaaagacct atacgggtgc agatgtggac tggtatgtaa aggcagagga tattgtgttg
    16141 aagaataaag gcaatcagaa cgcaggcagt ccacagaacc atataccgca gggacagccg
    16201 aaaaaatagg attgctgaat agattttcat tgtagcggat atatggaact gttataatat
    16261 ggatatgaag aaacgaacaa atttgaaatt gtcgcagatg tgttgggaga aagtgttatg
    16321 gttgggttta aagtgaagta ctattggttt caaataggaa gtgaagattt tctattttca
    16381 tttttttcaa cagttgctta taatttggaa aatcaaaaat ggggaactaa atttccagta
    16441 attatgaacg aattgtatca aggagaatta aaaagcacca gtattcctat ggcaataaaa
    16501 gaattaggaa gcataaagca aggattggcg aatttgccac ctgataagat aatatgggat
    16561 atagaaaact tatttgctaa accaccttgg gaaacaagta taagtagcga tataacaaat
    16621 ttatcgaatt attttgtgac aagtgatgga gaaaatttta tcacagtttt caaccacgca
    16681 ttagaaaaag caaatgagct taattgtgga gtggcaataa caatcatttg atatttgtac
    16741 aattttcagt ttgtataact gtattaaatc atgaaatcac attgaggtag cgattatgcg
    16801 atataaaaaa atcggattgg gtgttatagc aagtgtgctt tgtctaggac tgttgaatgg
    16861 ttgtagcggt tattcgcatg attttaactc tagtgaagaa gcacaaaaat atgtatcatc
    16921 caaattagaa gacaaataca atgaagaatt taccattacg gaagtgaaga agtataaaga
    16981 ggaaaaaatc ggtttaaact ggattatggt aaaagtttct agtaaagaaa agccctctca
    17041 aactgctact gtatatgcac gaaatactgg actttttgag gatagttatc atgtttatta
    17101 ctattctgat gagataaaag aacttgcaaa tcctttattt caagataagt cttttataag
    17161 agattatcaa cttgaagtac aaggacatac aaccactaca aaatggaatg gtaaggaaag
    17221 tgaagaagaa tatctgaaaa aaaaagagta tgaaatagaa tcacatatat atcttaatga
    17281 gggaaaaaca gatgaagaat atgcagaaga aatttcctgt atcatgcaag aaattataga
    17341 aagtgatttg gtttttaata tttcagtata tacaaatgat gatgacctta ttttctattc
    17401 tctaccagaa caacacagtc aaccagatgt cgaagttatt ttagaaaaaa tagctgatgt
    17461 tagaagacag caagaaacac aaaaaaatta caaggaatgg aaaaaacaaa atcagaagaa
    17521 tgaaaataat caagattaaa cactatgaat tattgtaatc actttaacga acaagaaaaa
    17581 tatcagtaag tgagattgaa agaaaggaaa ggagaagcaa tggataccga agtttttaaa
    17641 ttattatttt gtgtagcagc ttttggttgg attgtttata tggcttttaa agaaaaattg
    17701 cataagcaag atcctaaagc atttaaattt cttgcactga tatttgttgg gcttttgtta
    17761 gcttttatat gttttattat tccaggtgga gctaaatata catttgtact ggtgggtgtc
    17821 acaatgggag gaatgactac taagattttt tatcagaata ttaaatctgc tataatatgt
    17881 aacgaagcag ttaatggtat ctatcaagga tatagttcgt atcacagtgg aacagtatcg
    17941 agttatgcac ctatttttaa atataagtac aatggacaaa catatgtccg tcagtgttcc
    18001 aatacatata gttttaagaa attagataaa gagatggtta agggaaatga atatcagata
    18061 tatataaatc ctcaaattcc atcatcgttt gtacttaaca aaagagttgg tatcaaaaat
    18121 gtactgtgtc tgatgtttgg aataatgtgt atttttattg gcttatctgc actattcgtt
    18181 taaatatttt tttgtgctat gatgatactt gttgtaatcg tttttttaaa atgatagaat
    18241 gaaaacagta agttatgtga aaatcagttt tttaggagga caatataatg ctaaaagaac
    18301 aagatattaa acaatatctc aacgagaaaa atattgtatt taaggagaat acatctatta
    18361 ttggtgttat cttccctagt aaatttacat atgcacttgg tccagttgca acggctcttt
    18421 ctatgcaata tttcgcaatg aattttaatg ataatggtat tgctattatc ggtttaaata
    18481 acacaacggg taaactggaa aatgaagcat ttctttttgt tccaaaggaa gaaataacaa
    18541 gtgttaaatt caacaaaaag ttaatgtctt ttgaattgga aattattaca tcacaaggaa
    18601 cattagcttt taaagtgaac aaaacaatgg ttggtgcttc atggcataaa gaaaatttag
    18661 caacacttat atcaaattta taagtttttt caaaatttca tatccataca cataaagcag
    18721 ttagtcttag gattgactgc ttttttcgta tccagaaagg agtgattgat ttatgcgtat
    18781 gatttggaat aaaggacacc gtatcagagc cagcgataag caccttgtct atcacttttc
    18841 catagggtcg cttctgtttg tgttcgtggc ggttctcctg ctactgaata taaaacagct
    18901 catgcgtatt gattgggagc atttcagctt gttagagaat agcttgacgc ttgcccctta
    18961 caacttcata accatactga tagcgactgg tgtttgtgca ttggtcgctt ttctgtatta
    19021 ccgcttctgt tacgacagtt tcaagaagct cctgcaccgt caaaagctgg caagaatgat
    19081 actggaaaat aagtggtatg aagccgatac cgtgcaagac agcggttttt ttactgacct
    19141 gcaaagcaga tcaagggaaa aaatcgtctg gtttccaaag atttattatc aaatggaaaa
    19201 aggcttgctt catatccgtt gtgaaattac gctggggaaa tatcaagacc agcttttacg
    19261 gttagaggat aagttggaaa gtggcttgta ttgtgagctg accgacaaga ccttacatta
    19321 cggctatatc gaatataccc tgctttatga tatgatagcg aaccgtatta ccattgatga
    19381 agtacgggca gagaatggct ggcttagact gatgaaaaat cttgtctggg aatatgacgc
    19441 acttcctcac gctctgattg ctggtgggac tggtggcggt aagacttatt tcctgctgac
    19501 gctcattgaa gccctactgc ataccaacgc tgtcctttat atcttagacc cgaagaacgc
    19561 tgaccttgcg gacttaggga cagtcatggg aaatgtgtat cacaccaaag aagaaatgat
    19621 agattgcgtc aatgtctttt atgagggaat ggtacagcga agtgaggaaa tgaaacgaca
    19681 cccgaactat aagacgggcg aaaactacgc ctatctggga ctgccaccct gctttcttat
    19741 ctttgatgaa tatgtggcgt tttttgaaat gctggggaca aaggaaagcg tgagcctact
    19801 tagccagtta aagaaaatcg tcatgctggg acggcaagca ggctattttc ttatcgttgc
    19861 ctgccagcgt ccagacgcaa aatatttctc ggacgggata agagataact tcaatttccg
    19921 tgtgggattg gggcgtatca gcgaattagg ttacggtatg ctgttcggtt cagatgtgaa
    19981 aaagcagttt tttcagaagc gtatcaaggg gcgtggctat tgtgatgtgg gaacaagcgt
    20041 cataagtgaa ttttacacgc ctttagtccc gaaaggacat gattttttgc agactatcgg
    20101 caggcttgca aaagaaagac aagttatgcc agaacaacaa gaaaaggaga actaactcat
    20161 ggagataaaa gaaatcaata aaatattaca accagaagat aaaggagaaa aagctaagag
    20221 tgtaaaatgt atctgtatca attatggaga ttttctagcc gattgtgagg gctttgtgca
    20281 gaaacgctat cacgatttta aatgtaatcc aaaacatcag tttgagaaaa aagctgacac
    20341 tattctcgaa aatgcaattc acgaaaaaaa ctttatgccc gatttgttct tgatacgctt
    20401 aaatagaaaa caatcggctt gcaacagcca gattgatttt gtttttgaat tgctggataa
    20461 gtcttttctg gaaacagacc ctatccgtaa aagtgaaata tcagaagaaa cattgaattt
    20521 atgtttatcc gctgatgttt cgttcatcat ggtttacatt ggtatgaaca ggtaggataa
    20581 gttctgtctg cacaacggca ggaacggtcg gcggagcaaa tacgaccgtg gaagccgttt
    20641 tgcagagcag ggcttgtccc tgctctgctg attaacccct cggtatctaa cagaggggta
    20701 cgtttgcaaa tagacaatag acaaaaaaca taggaaacat aaggcttctg gcatggtttt
    20761 ctaggaaaat tcagttgtga agtcgcctta aaaaacttca cactttacag attgggggta
    20821 tggttctgaa tgaagaacaa tggataaaag aattacggga gaaacggatt gcttacggta
    20881 tttcacaagg caggctggcg gtggcttctg gtatcacaag ggaatatctc aataagatag
    20941 aaagcggaaa aatgaagccg tcaaaggaac ttctggaaac tctgcataaa gaactggcaa
    21001 gattcaatcc agaagcaccg cttaccatgc tgtttgatta tgtgaaaatt cgttttccca
    21061 cgctggatat acagcacatc atcaaagaca tattaaaact gaatatcaat tatatgctcc
    21121 atgaagatta cggacattac agttatacgg agcattactc tttaggggac atctttatct
    21181 atacgtcggc tgacgaagaa aaaggtgtcc ttttagagtt aaaggggcgt ggttgcagac
    21241 aatttgaaag ttacctgctg gcacagcaaa gaagctggta tgacttcctc atggacacac
    21301 ttgtagacgg tggagtgatg aagcgtatcg accttgctat caacgaccat acaggcattt
    21361 tggatattcc agagcttgcg gaaaaatgca ggaaacggga atatatcggg aagtccagaa
    21421 gttataagtt ctaccagtcg ggcgagctta tcaagcacag agaggacgac agagaatata
    21481 tggggcgtac cctttatctt ggttcgctga aatctgatgt atatttctgt atctatgaaa
    21541 aggactatga gcagtacgtc aagctgggga caccgctgga agaagcagac attatcaacc
    21601 gttttgagat acggcttcgc aatgaacgtg cttattatgc agtacgagat ttgctgacct
    21661 attatgacgc agaacagacc gccttttcta tcatcaatca gtatgtacga tttgttgatg
    21721 aagaacccga caagcgaaaa aataactgga aactcaatga ccgttgggct tggtttatcg
    21781 gagataatcg gcaaagtcta aaactgacga caaagccaga accttatacc ttagaccgta
    21841 cattgcgttg ggtacaaagg caggtagcac cgaccttgaa aatgttgaaa aagattgatg
    21901 aaggaaacgg tacagactat atggaaacaa tcgaacagca ggcaaagctc acagaaaagc
    21961 atgaaatgat aatcagacag cagacgaccc ctgcaaaaga tttagtagaa agttaggagt
    22021 gataaaaaat gtgggtatta ttaaaagact tcctgctggt atctatggga atgggtatcg
    22081 gcgtagtctt gatgtgtatt ttgaatgtcg gcaaagaagc tgactatgaa atgaaacaat
    22141 taaaagaaag cgaggacaat taaatgaatt tcggacaaaa tttatatcaa tggtttttat
    22201 caaacgcaca gagcttagtg cttatggcga ttgtggttat cggtatctac ttagggttca
    22261 agcgtgagtt ttccaaactt attggtttct tggtagttgc cttgattgct gtcggtttgg
    22321 tattcaatgc tggcggtgtg aaagatgtac tgttagagct gttcaataag attattggag
    22381 cataggaaca tattgaaacg ctataagctt cttgatataa ttaaggaaaa gtgtggaggc
    22441 gttttttatg atgagattag atttacgtga tttacatata gatattttaa agcataatat
    22501 gacttatgat gtatttgatt attctcataa tggtatacaa tttaacattt tgtttgatgt
    22561 aaactgtatt cctttcaaat tgatttttat aaagaagaaa agtggacaat atctaatttt
    22621 agatgttttg gctggttatc aactaaatac ttatttagat gaaaaattaa aaatcttaaa
    22681 agatatgttg gaattaaaaa atggaaaaag taagttttcc acaaaccaat tctttgaaga
    22741 atttaacaaa aagatacctc atactattca gaataagaaa ttgtctaaag caacaattag
    22801 tcatgtatat cattgtgaag aaagtgaaaa aatttatatt cgtgaattgc gtaattggga
    22861 taaatatcca gaattaggaa aacacgctac aaaagaaaac aaggaaaaaa caagattatt
    22921 atatccagat atttatgaag aaataaagga taaaaatatt agtgtgtttt atactgataa
    22981 agaaaaatag agaaaagcag ttagtcttag gattgactgc ttttttctta ccaaaatttc
    23041 agattggagt gatgaaatga gcgatatttt taaggatatg caggagaaag tcggctgtga
    23101 atacatttcc gacctgccct cttacaagcg taaggtgtgg catgaaatga aacggctgaa
    23161 ccctgccgac tatgaagaaa gtcagttaga agatttttca aagtatgtgt ttggtatgtc
    23221 ataccagacc ttaaaagatg tgatgaaaca gcagaaagga cgtgaggaac aatgcaggaa
    23281 acaagggtgc tggtggaaac gagaggaaca actggcgaag aaacaacatc atactggttt
    23341 gacctgccga tagatgttgc cgagtttgaa gaaaagttga gtgtcggtgc agaaagtggg
    23401 gagtaccgca ttatcgaaaa ggtgttgcct tatgctgatg aagtccacga acatacaagc
    23461 gtatatcagc ttaacgaatt agattttatg taccgccagc tttcaagtga tatgcaggaa
    23521 gaatatacag cacttctgac cgtatttgag aatttggaag cactttatat ttgcaggaac
    23581 gtgattaccg tttatcccga ctgcaaaagc atgatagatg ttgcaaggca aaagctgatg
    23641 aacgacccga cgtttaaaca tctgtccgag gactgtcaag aatattactt tgactttgaa
    23701 gcctacgctt ctcacttgca ggaacacggg aaatttttag taacagaaca cggtatcttt
    23761 gaactgccag agtaggaaat gaggtggtgc ttatgattga tgatatggcg gtttatattg
    23821 ctaatcttgg caaatacaat gagggctatt tagtgggggc ttggttcacg tttcccattg
    23881 acgaggaaga tgtaaaagaa aaaatcggtt tgaatgaaca gtacgaggaa tacgcaatcc
    23941 atgataccga taacttcccc attgcgattg gcgagtatgt ttccattgaa gaactcaatg
    24001 atatgtatga aatgatagag gaacttcccg actatatcgt agagtgtctg gacgaattta
    24061 tcagccacta tgggacgctg gaagaagtcg tggaacataa ggacgatatt tactattatc
    24121 ccgactgtga aaccatgaca gatgttgcct actactacat agacgaattg caggcacttg
    24181 gggacattcc acccagctta cagaactaca ttgactatga agcctacggg cgagatttgg
    24241 atatgggcgg ttgctttatc gaaacaagcc gagggatatg cgagatacca tattaacgct
    24301 ggaagatcag caacaaacat tgttgctact gacagcgtat ttctctttaa gggacagtct
    24361 gaaacatggc tgtccttttc tctttgaaga acttacgaaa ggagctgaaa gaacttgaaa
    24421 aagattaaat cttatacggg tatctggaac gtggaaaaag tcttgtatgc aatcaatgac
    24481 tttaacttac cctttcccgt tacttttaca cagattacat ggtttgtgat tacagaattt
    24541 attatcattc tgtttgggga tattccccca ctttccatga ttgagggagc atttctcaaa
    24601 tactttggta ttcccgttgc tctcacttgg tttatgtcac agaaaacctt tgacggaaag
    24661 aagccgtaca gctttttgaa atcacagata acctacgcgt taagaccaaa aatcacttat
    24721 gcaggaaaag ccgtaaaact gcataagcag atattgaatg aaacaatcac ggcagtaagg
    24781 agtgtgaact atgttcccga taaaatatat tgacaataac tttgtctgga acaaggacaa
    24841 tgaggtgttc gcctactatg agctgatacc gtacaactat tctttcttat ccgcagagca
    24901 gaaatttatc gtgcatgaca gctttcgaca gctaatcgca cagtcccgtg agggtaaaat
    24961 tcatgccttg cagattgcca cggaaagctc catacgaagt atgcaggagc agtcaaagaa
    25021 actggtaact ggaaaattaa aggaagttgc ctgccagaag atagacgaac agaccgaagc
    25081 gttggtatct atgattgggg acaatcaagt ggactaccgc ttttttcttg gctttaagct
    25141 catggttacg gaagaacagc tcaatctgaa gaacatgaaa aaatcggcgt ggctgacgtt
    25201 cacggaattt ctccatgaag tgaaccacac gctgatgaac gattttgttt ccatgccgag
    25261 tgatgaaatc aaccgttaca tgaaaatgga aaagttactg gaaaataaaa tctcccgtcg
    25321 ctttaaggtg cgtcgcttgg aaatcaatga ttttgggtat ctcatggaac atctttacgg
    25381 cagggacggt atcgcctatg aagattatga gtaccagcta ccaaagaaaa aattgcagaa
    25441 agaaacgctg ataaaatact acgaccttat ccgtccgaca aggtgtgtga ttgaggaaag
    25501 ccagcggtat ttacgattgg aacatgagga caaggaaagc tatgtgtcct actttactgt
    25561 caatgcgatt gttggcgagc ttgattttcc gtcgtctgaa atcttctatt tccagcaaca
    25621 gcaattcacg ttccccgttg atacttctat gaatgtagaa atcgtggaga acagaaaggc
    25681 gttatccact gtccgcaaca agaaaaagga attgaaagac ttggataatc acgcctatca
    25741 agctggcagt gaaaccagtt ccaacgtggt ggacgcttta gacagcgtgg acgagctgga
    25801 aacggattta gaccagacca aagaaagtat gtataagtta agctacgtca tacgggtgtc
    25861 tgcacccgat cttgacgagc tgaaacgccg ttgtgatgaa gtcaaagact tttacgacga
    25921 cctcaatgta aagctggtgc gtcctgctgg cgatatgtta gggcttcatt ctgaattttt
    25981 accagccagc aagcgatata tcaatgatta tgtgcagtat gtaaaatcag acttcttggc
    26041 agggcttggt tttggagcaa cccagcagtt aggggaaaat acgggtatct atattgggta
    26101 ttccgttgat acgggaagaa atgtgtacct gcaaccgtcc cttgccagtc aaggtgtgaa
    26161 aggtacagtt accaacgctt tagcttctgc tttcgtcggt tcgcttggcg gtggaaagtc
    26221 attctgtaac aatctacttg tatattatgc ggtgctgttt ggcggtaaag cacttctgtt
    26281 agaccctaaa agtgagcgtg gcaactggaa agaaacgctc ccagagatag cccatgaaat
    26341 caatatcgta aaccttacca gcgataagga caatgcagga cttcttgacc cgttcgtgat
    26401 tatgaaaaat gtgaaagacg ctgaaagtct ggcaatcgac atcttaacat tccttacggg
    26461 tatttcctct agggacggcg aaaaatttcc cgtacttcgt aaagcagttc gttccgttac
    26521 ccagagcgac agtcggggct tgctccatgt aatagacgag ctacgccgtg aagatacacc
    26581 agtatcaaga aatatcgcag accatatcga aagtttcacg gactacgact ttgcacatct
    26641 gctattttca gacggaacgg tggaaaatgc aatcagcctt gataatcagc ttaatatcat
    26701 tcaagtagcc gaccttgtac tgccagataa agataccacc tttgaggaat acacgaccat
    26761 tgaattatta tcggtgtcta tgctgattgt gattagtacc tttgccctcg acttcattca
    26821 cagcgataga agcattttta agattgtcga tttggacgaa gcgtgggcgt tcttaaatgt
    26881 ggcacaaggc gaaaccctat caaataagct ggttcgtgct ggacgagcta tgcaggcagg
    26941 cgtttatttc gttacccagt ctgcctatga tgtttcaaag gaaagtctga aaaacaatat
    27001 cggcttaaaa tttgcattta gaagcacgga tataaacgag ataaagcaga ccctagaatt
    27061 tttcggtatc gacaaggacg acgaaaacaa ccagaaacgg cttcgtgatt tggagaacgg
    27121 acaatgttta ttgcaggact tatacgggcg tgtcggcgtg gtgcagatac acccagtctt
    27181 tgaagagctg ctacatgcct ttgataccag accgcccgta cagaggaatg aggtggagtg
    27241 atgaaagaaa ggataaaagg tgcgttcaca aaaaagaaga ttttccactt tctcaaaatg
    27301 gctctgttcg tcgtggtact ctcccttatc ttgctttcac ttttggggac agtggctcat
    27361 gcgacggggc ttgtggacga caccatcaac gcagaaaatc tttattctaa atatcccctt
    27421 tccaactacc agcttgattt ttatgtagat aatagctggt cgtggcttcc gtggaactgg
    27481 ctagacggta tcggaaaatc ggtgcagtac gggctttact gcattaccaa ctttgtctgg
    27541 acgataagcc tttatttaag caatgcaacg ggctatgtgg tacaggaagc ctataaactg
    27601 gacttcatca atgatatggc agacagtatc ggaaagagca tacagaccct tgcaggtgtt
    27661 acgtcaaacg gtttttccag ctctggcttc tatgttggtt tcctgcttct cattatctta
    27721 gtggtaggac tttatgttgc ctatacggga cttatcaaac gggaaaccag caaggcactt
    27781 cacgccgtta tcaactttgt cgtggtattc attctgtccg cttcgtttat tgcctatgct
    27841 cccgactaca tcaagaagat aaatgaattt tcatcagaca tcagtaccgc ttcacttgac
    27901 ttgggaacaa aaatcatgct ccccaactct gacagcgagg gcaaggacag cgtggacttg
    27961 atacgggaca gcttattctc tattcaagta caacagccgt ggctactctt acaattcggt
    28021 aacagcaacg cagaggaaat cgggacagac cgtgtagaag ctctggtatc ggtaagccca
    28081 gaggacgagg acggaaagac tagagaagaa gtcgtgaaaa cagaaatcga ggataacgac
    28141 aataacaatc tgacgatacc gcaggtggta aatcgtttgg gtatggtgtt cttcctcttg
    28201 ttttttaact tgggtatcac gatatttgta ttcttgctta cgggtatgat gttgtttagc
    28261 cagatacttt ttattatctt tgcaatgttt ttacctatca gctttttact ttccatgata
    28321 ccgagttatg aaagcatggc gaagcaggca atcgtaaggg tatttaatac cataatgaca
    28381 cgggcaggaa taacgctcat tgtaacggtg gcgttcagca tttccagtat gttttataac
    28441 atttccacag attacccgtt tttcatggtg gcgttcttgc agatagtatg tttcgctggt
    28501 atttacataa agctgggcga cttaatgagt atgttctcct tgaacgctgg cgacagtcaa
    28561 agcatgggac gcaggatatt ccgcagacca tatttgttta tgcgacacag ggctaggcga
    28621 atggagcgac gcatggcaag agctgtcagt catggaagta tggttggagc tggcacaggt
    28681 gcggttacgg gagctatgct atcgggtaat ggttcttcaa ggagtaatcc tcaaaaagat
    28741 aaacgtacct tttcacgaag caacacttct tccagttttg gaaatcgtgc tggttctgct
    28801 gtgggtgctg tcttagatac gaaaaataag gtcaaagaca aggcaaacgc tgtcaaagag
    28861 aatatcaagg atatgccgac acagactgct tatgcggtgt attccgcaaa ggaaaaggca
    28921 aagtccagcg tgtccgactt taagcgtggc atggtgcagg aacagcagtc cagacagacg
    28981 ggacgtttgg aaaagcagga acagcataga caaaatatcg ctgacaagcg tatggagctt
    29041 caaaaggcac aagaagcaag gcaggcacag cgaaaggctg acggatcagc gacaacggga
    29101 gctacccgtc cccatgagcg accagccaca gcttcaatca ttccaaagcc gagtgctgaa
    29161 aaaacgcagg aagtcaaacg tcctgccaca gcgaccactt caaaagcaag tgagccagtt
    29221 aaaacaaatg ttatcaaaga gcgtccgtta tcctctggtg cttctgatgt aaaagcgacc
    29281 cagtcggtac agcctgcaca taggcagaat gtagaaaaag tggtatcgca ggaaacacgc
    29341 cagaattaca ccaaagaccg caggacaaag gttcagcaga cccagaacgt ccagaagaac
    29401 cagcagacca cagagaaaac ccgtaacctt gtaacgaaga aaggacagaa gaaaaaatga
    29461 aactgaaaca tatcgctctc attggcagtc tgtttcctat cctcttttct ctggtgcttt
    29521 tctttggtgt cttgattagt gcggacagcg acgacgagaa cagcaacttt tcttctggca
    29581 ttacgggtat gaacttatcc gcagaagtct tgaaacatca gcctatggtg gaaaaatatg
    29641 ccagagaaaa cggtatctcc gagtatgtca ctgtgttact ggctatcatt caagtagaaa
    29701 gtggcggtac ggcagaagat gttatgcaga gttcggaaag tcttggttta ccgcccaact
    29761 ctttagatac agaaagctca atcaagcagg ggtgtaaata ttttgcgtcc ctgctttctt
    29821 cctgcaaaaa tcaaggtatc gacgatttga atgtagcgat acagtcttat aactatggcg
    29881 gtggctatgt aggatatgtg gcagggaaag gaaagaaaca tacatacaat cttgcagaga
    29941 gcttcgcccg tgagaaatcg ggtggaaaga aagtaaccta taccaacccg atagctgttg
    30001 cgaagaacgg gggctggcgg tatggctatg gaaatatgtt ctacgtcgaa ttagtcaatc
    30061 aatatctgac cgtggcacat tttgataatg cgacggcaca agcgattatg aatgaagcgt
    30121 tgaaatatca aggctggaaa tatgtgtatg gtggcagtaa cccgaacact tcctttgatt
    30181 gtagcggact tgtgcaatgg tgctatggag aagctggtat ctccttaccg agaacggcac
    30241 aagcacagta tgacgcaacc caacatcttc cactttcgca ggcaaaggct ggggacttgg
    30301 tattcttcca ttctacctat aacgctggtt cgtatgtaac gcacgtcggt atctatgtag
    30361 gaaataacaa aatgtatcat gcaggcgacc cgataggata tgcagaccta agtagtagtt
    30421 actggcaaca gcacttaatc ggtgcaggac gagtaaaaca atagaaagga cttgaaaata
    30481 tgtttaagaa aaataagaaa cagacagaaa ctatcaaaga accaaaagaa aaaaaggtgc
    30541 gtactgtcaa ggtaggcaca cataagaaaa ccgtgattgc gttgtgggtg gtgcttatcg
    30601 caagtgtgag ttttggggtg tataagaatt ttaccgctat cgaccagcac acgacccatg
    30661 aaaaagaaat cattgaactt cgcttacagg acactaacgg gatagaaaat ttcgtgaaga
    30721 actttgcgaa gtcctattat acatggagta acagcaaaga agcgattgac gcaaggacgc
    30781 aggcaatcag tggttatctg acaaaggaat tacaggactt gaatgtggat accattagaa
    30841 ccgatatacc gaccagctcc acggttacag atgtgcttgt gtggaacatt gaacagtcgg
    30901 gaacggacac cttttctgct acctacgaag tagatcagca gataaaagag ggagaacaga
    30961 caagcaatgt gaaagcaacc tataccgtaa aagtccatgt ggacgctgac ggggatatgg
    31021 taatcgttca gaaccctacc cttgcaccag caatcgaaaa atcagactat gaaccaaaga
    31081 caccagaagc agacgcaagc gtggacgctg ataccgcaaa tgaagctacc gcatttctgg
    31141 aaacattctt taagctctac ccaacagcca cagaaaaaga gcttgcctat tatgtatctg
    31201 gaaatgtgct tgaacctatc ggcagggact acctttattc tgaattggta aacccagtat
    31261 ttacaaagga cggggacaat gtgaaagtca aggttgctgt gaaattcctt gataatcaga
    31321 caaagtcgac tttagtttcg cagtatgagc ttgtgctaca taaggatagt aactggaaga
    31381 ttgtgggata actgacataa aaagaccgta gtctgtaata cacatttatg tgccttacag
    31441 ctacggctat ttttgaaatt gattgcgtat atctttgtct ttacggaacg ctattttagc
    31501 agacttcatc aaatctacta aaaaacgtac ttcgtattca tcacagtccg aaaaaatatc
    31561 ttttgcttct ttttcaaaaa caattttaga agcaatcaca ttatcacata aaagagtatc
    31621 gactgataca gaaagtgcat tggcaatcgc aatcaaagtt ggtaaactga cttttgtttt
    31681 tcctgtttct acattactca tgtgagctgg cgtaatatct ataaggtcgg caacagcttc
    31741 ttgtgttata ccctttttaa ttcgtgcaat tttaatacgc tgtccgattt ctttgtaatc
    31801 aatttccaca gtttccactc ctttcttgct tttttatatt gtaaccattt gcagagtgta
    31861 atataatact ctgtataaat gaatatttta cacaatatat ttttgcgttg atttttttta
    31921 gatactatgg aaatttcaaa agaaaagggg gtagtctgta tgctaagatt taagaaaaaa
    31981 gacagcaaag aacttctaaa aagagtagca aaaatacatg gttgttcggt atctgaaatt
    32041 cgtgaggaaa tgcagtatgc gattgaagaa gcacgaaata atgacgaccc agacaaacaa
    32101 gctgaatttc aacgcttatt tggaaatagg acacctagcc cagaagaatt tatttatacg
    32161 attagcaaga aattaaaagt ttagtaaata cggagtgtgt gtgatgaaaa aagcagaaat
    32221 gcaggaacag gaatttttat cagtctgttc ttcttatgat tacgaccttt taacaggaaa
    32281 aagcgaaatg acattgaaag actatgaaag aattacttat ttagttagtt ctcttgggta
    32341 tagtcaatat cttcaatttc ttattggaaa atatatggac ttggcacaag ctgacgaaga
    32401 acgagaagaa agagaacatg aaatatattt agaatatcca gagtattatg aggacgaggg
    32461 aattttggaa gaagaagaaa aatggttgga agaattttta aatcaacttc cagagaataa
    32521 aagagaatat tttgaaagac ttataaaaga aaatcaaagc tactaaaaaa taaagttact
    32581 gtattcagat gtgctgatca cagtaacttt tttgcgttgc actaaatttg aagataaact
    32641 atatattttg tgttaaaatt agttttaata gagaattata aattgacgaa aagttgactt
    32701 ttatgtgata taaagtttct aatcttgttg ataaggagtg aaaaccattg gaagaaaaat
    32761 ataaaatttt aattgtagat gatgaacagt ctattttaga tatgctgaaa ttacagcttg
    32821 aatttgaggg atattctgtc tatattgcaa acaatgcgaa agaggcttta gataaattat
    32881 cttacttgcc agatattatt ctgttagaca tcaatatggc tggtatgaat ggcttggact
    32941 tatgtgtatc tattcgtgat tttatctctt gtccgattat ttttcttact gccagagtat
    33001 cagaacagga taaaatcaac ggacttatgg ctggtggaga tgattatatt acaaagccgt
    33061 tttctgtaaa tgaattgctg gcaaggattt cagcacattt acggcgtgaa cagcgaagtc
    33121 ataataagac gaaaagtaaa ttttcagaag aacttattat tgattattcg gatagaagcc
    33181 tttatatcaa aggaaacaaa attgaattgt caaataaaga atttgaaatt atacaattac
    33241 tatcttcaaa tgcaggacag gtttttgaca gagaaaaaat ttatgaatgt atctggggca
    33301 ttgatggtaa tggagatagt gtcgttatta aagaacatat acgaaaaata aggctgaaat
    33361 ttagtgaatt tacagataaa gtctatatta ctacggtttg gggagttgga tataaatggg
    33421 aaaaataaaa aagacttttc agaaaatgtc attaaaaaaa tctcttttag tattatctgt
    33481 attctatctt agtctggtta gtgtactctc gattgttacg attttgaagt gttctaatat
    33541 tcggcaggaa atacttgata ccagacctat tattattaca gactatatca ttgacgacac
    33601 aaatataaat ggtgcagaaa ataataacgg agttactgcc gtaccgaaaa aatacacgca
    33661 cggcgaactt acaaaagaaa atcaagtgta ttattggtgc attactattc ttatggtatc
    33721 tttgcccata gcttatatta tacttgcttc ttttatggtg gcgaaattat attacaaatg
    33781 gaaattacag ataccgcttg aacatctaaa aaatggtatg tatcacattt cacagcagga
    33841 cttagatttt caaataccat atacttctaa tgatgagtta ggacagttat gtgatacgtt
    33901 tgaacacatg aagaatgaga tttatagaag caatcgtaaa atgtgggata tgttacaaga
    33961 aagaaaagct ctgaccgctt ctgtttcaca cgatttgaga acaccgatta cagttatcaa
    34021 tggctatctt gattatttag aaaaatcaat cgaaagagaa acccttacaa gtgaagtttt
    34081 acaagcaacg attgaaaata taacaggtgc aacagaaaga ttagagagat atgtagattg
    34141 tgtcaaagat atacaaaaaa tggaagatgt tgaaatcaaa atggataact atgatttgaa
    34201 agaattgatt gctggtatca caagagaatt ttctgttttg gcaacacaac aaagtaaaca
    34261 atttagtgtg catgatttat cgaaaactgc ttttattcag acagacaaag atatgctgtt
    34321 aaaaatttta gaaaatattt ttgataacgc acttcgcttt tcttctgaaa aaatcatgtg
    34381 tttaatcgaa gaaaaagagg aatatcttta tttttctata caggacgatg gtgtagggtt
    34441 tacatcagaa gaattaaaat ctgcaacttc tttctttttt agctcatcta caaacggtgg
    34501 aaactttggt ataggtttat caatttgtaa aatactttgc gaaaaattgg gtggggatat
    34561 gtgtttagat aataccgccg aacacggagc tattattacc attcggataa aaaataatcg
    34621 aaaacttttc taaaaattga cttttatatg aaataatctc cttacagaag atgtaaggag
    34681 gtttttttat ggaaattaag attgaacact tgaataaaaa ttacgggaaa caacgtgcct
    34741 taaaggatat atcattacat atcccagtcg gtatgtatgg tctgttagga gaaaacggag
    34801 ctgggaaaac aacccttatg cgaatattag ctacattatt ggaacagtca acaggaacgg
    34861 tagaaatcaa tggaattgat ataaaaagaa aaaaagaaat tagaaaaata atcggctatc
    34921 tcccacagga attttctgta tatcctaata tgacggttta ttctgcttta gattatcttg
    34981 gaattttagc ggaacttcct aaggatattc gtaaaagccg tgtcagtgag cttttaaagc
    35041 aagtgaattt agagcaagag aaaaataaac gattcaaaaa tttgtctggt ggcatgaaac
    35101 gccgttttgg tattgcacag gcattattaa gtaaccctca aattttgatt attgatgagc
    35161 caacggcggg acttgaccca gaagaaagat tacgctttta taatcttctt tctgaattag
    35221 caatggatag aattatttta ttatccacac atatagcaag tgatattgaa gctacctgct
    35281 ctaatgtagc tgtgctatct tctggaactg ttatttttga aggacaagta aaaaacttgt
    35341 tacaacttgg atatagaaaa gtctatacca ctacgattat gcaaaatgaa ttaagtcagt
    35401 ttaaaaagaa acaccatgtt attagtattc atcaaaatgg aacagatgta tcttgtcgtt
    35461 ttttatcaaa cacctctcca caaaaagaat gggtttcctg tcaacctacg atagaggaca
    35521 gttatatgta tctcttatca agttttaagc agggggtgga ataaatgcta tttatcaaag
    35581 acttgaaaaa aatatgtttt agttttgtat atattctttt tgttggatta cttctgttta
    35641 gctggaatga aaatttttat ggtgttacaa gcaaagaaat acaagcgtct aatggaactg
    35701 atacttctat tacctcacag ataacaggtg tgtctatttt agccaaacca gaacagggtg
    35761 cagaaaatta cggctcaata agaaaagaag tgccagataa aatcatgtgt ggtggaacag
    35821 atatgctaat tattgaatat ttgaaaaatt cttatgccac atatcctttt aattattata
    35881 aggaagtgat tttaagtaca caggagtaaa aagaaatctt atctattatt gaagaaatca
    35941 caggtttatc tgaagaacag ataaaaaatc ttccagaaga ttactttccg tccgtaaatg
    36001 gaaatattat ccatataggc ggggatgttt cacaaaatca agatgagagt ttcagctttc
    36061 aaacggggaa taacgatact ttacaagatg aaaaagatta tacgaagcat tttatagcac
    36121 aggtttcata cgaaagattt aaagaactta tggcaaaagc agaggaaata ataggatctg
    36181 gctctaacta ttctatggat atgttattgg aatactacgg gcaagttgaa atgacctatg
    36241 atgaagctat gaatgagtat aataaaacga tttatgatga taaagtcagc accgcttttg
    36301 caagattgtt ttgtgattat atgacaagag atttaggctt ataccctgtg ttcttagtag
    36361 ctatcttctg gctaaaagat agacgaaacc gtatgaatga gttgattgac tgtaaacaga
    36421 ttgggacagc aaaacttgtc gcaattcgtt tcttagcaat gcttgtagct gttatgatac
    36481 cgatatttgt tttgagcttc gagtcgttaa taccattgat gaagttttct actgatacgg
    36541 gtatcgcaat agatgtgttt gctttcctaa aatatatagt atggtggcta ctaccaacag
    36601 ctatgattgt tacttctttg ggaatgtttt tgacaatctt aacatctaca ccgatagcta
    36661 ttttagtaca gtttgtatgg tggtttattg actctgctat tacagggctt tctggagata
    36721 tcagactata tactttgatg atacgtcata acacgctaaa tggagcagaa cttgttcagc
    36781 aaaattttaa tatcatttgt cttaacagag ggataatgat aattttatcg ttcattctga
    36841 tttgtttatc catagttatt tataacaaga aacgaggtgg aaaattagat tatggatact
    36901 tcatgcaaaa acattttgga gtttttaaaa atagacttct ggctcgtttt caaaaatagt
    36961 ttgttacttg cggttgctta tacattcctt atccctgtga taaggggaat ttcaaattta
    37021 gatgccgccc attccgcaga tgtattcgga caatcgctcg ctctgatagg agtattcctt
    37081 ttcgttccta taacaagtca agaattggaa agtagcataa aagagattgt ttatacgaaa
    37141 gtatggtcat ataaaaagag cgtaagtctt cggctgatat gcggttttct gctgagtacg
    37201 gttatagtta caatattcgc tggtatcatg caattaaaaa actgcgactt cccattttta
    37261 gaatatatat ctgtaacaat tttgtatgct gtttttctgg gactgctagg acttcttttt
    37321 tcacaagtgg gaaataatgt cattgcagga tacttagttt cattggggta ttggtctttc
    37381 tgccaacttc atattttagc agaagaaaat ttgatgtatt tgtttccgat tataaatgga
    37441 gaaatagata tggaaaaatt gctaattctc gtaattgtaa atatatgttt actggtgctt
    37501 ttcttttgga tagtaaaaag aactaaagga aaagtttaat gaatattatt taaaggagat
    37561 tatcttatgg aaagttttaa acagaatact atacgttcca acacagtata atcttaaaat
    37621 aattgcatat ttcagttttt tagctcgtac agactatttg ttttgtgcgg gcttttttgt
    37681 acccgaaaaa ttttttcaac ttttttcgac ttcaacttag caaatcacac ttttcattcc
    37741 ctatatggtg cggaaagagg gataaacact tttcacgtca taacggaaag ggggtgagat
    37801 tgatgaaacc gtctgacttc cagaaaactg ttcaatgccg ttttgagagt tgtttgaaaa
    37861 aagttgtccg tcatgtcgta aaggactatc agcagggatt gaagcgacga aaagaaagag
    37921 aaattccatt ctgtgaactt ccagaaatcg ttgtggaaaa gttggctgtt tgggacgaat
    37981 acgacagcga ttatacactt ttcaacgtat gcggtagtga tattcgtatt tgtgatgatg
    38041 aactggcaga agccttgaaa catttgtccg agcgtaaccg agaaaatttg ttgatgtatt
    38101 atttcttgga aatgagcgat accgagatag caaaaagaca aaatatttca agaagtggtg
    38161 tttttcaaaa ccgacacaat tcactagaac ttatgaaaaa aaaattaaag gagaaaagat
    38221 agcaaatgaa acacacaatg aaaaagccgt cctattattt gctttcacag gctatggacg
    38281 gggacgaaaa agcgattgga aagatactgg cattttatga cccgtacata tcaaagtgct
    38341 gtctgcgacc actctatgat gaatacggca atgtttatat tgttgtagat atggagctaa
    38401 aaggacttat cagagaagca cttattaaaa tgattttagg gtttgatatt gccttagaaa
    38461 tcgaagaaga ataacaagca gtaaccgcag agcatgattt gttcaatccc ctctttcctg
    38521 ctctgctccc tgcttttaca tgaaagcaac acgctttcgc cacagttctt tgacaactga
    38581 ataaagcaga cagatacgtt tgtgagatag cgagccacgg ggactgtacg ccacgacatt
    38641 ttcaaaagaa agcgagcgac ccacgcagtg atccgagagc gactgttgga aaagtcgttt
    38701 tgccacgacc tatcttcaca gaataatgat acgtccgcat ggtgcggttc tgcccacaag
    38761 aatgggaata gttgagatac taacggagct ttccaaagcc gtctgcctgc ttgtaaaaaa
    38821 tgacacatag taaagggggt gcatagatta tgaacaatac tgatgtgcct atctgggaaa
    38881 aatatacgct gacgattgaa gaagcgtcca aatacttccg cattggcgag aagaaattgc
    38941 gtaaattagc cgaagaaaat cttgacgctg gctgggttat cgtgaacggt aatcgtattc
    39001 agataaagcg aaaacaattt gaaaaaatca tagatacact ggacgaaatc taatgtcatt
    39061 gagccgtgaa tatggtataa taagatatag cgtatcacgg ctcattccat gacggaaagg
    39121 agctttatac tatgtctaat gtaaaacgaa aagacagtaa aaatcgcaat ttacgaaatg
    39181 gagagagcca gcgaaaagac ggaagatacg tttataaata taccgatata tacggaaagc
    39241 cacaatttat ctattcttgg aaacttgtac cgacagacaa gacacctgct ggaaagcgtg
    39301 atgatatatc gttgagggaa aaagaagcac agataaaaaa agaccttaac gacggtatcg
    39361 acacagtcgg cggtaaaatg acagtctgcc agctctacga gaagaagaac agccaaagaa
    39421 agaacatcaa gagggctact gaaaaaggac gacagtatct tatgaacgct ctgaaaaatg
    39481 acccgttggg tatgagggcg attgataccg tcaagcagtc ggacgctaaa gagtgggcta
    39541 tcagaatgag tgaaaaagga tatgcctata aaacgattga taactataag cgttccttga
    39601 aagcgtcatt ttatatggcg atacaagacg actgtattag aaagaacccg tttgaattta
    39661 agctaagtga tgttctggaa gatgataccg aacagaaagt tatccttacg ccagagcagg
    39721 aagaaagcct gcttgccttt atggaaacgg acaagattta tagcaagtat tatgatgagg
    39781 ttgtgcttct gttggaaacg ggacttcgta tttctgaatt ttgcggattg acgacgcata
    39841 ttgatatgca gaacagaata ctcaatatag accaccagtt attgaaagat agcgaaatgg
    39901 gctactatat tgaaacgcca aagaccaaaa acggaaaacg ggaacttcca ttgacagaac
    39961 gggcttatca agcaatccaa agaatactag agaacagagg aaaggcgcaa ccgctgattg
    40021 taggtggtta cagcaatttc ctattcttga accgtggggg cttgcctaaa gttgcaggaa
    40081 attatgaggg tatggtgcga ggactaatta agaagtataa caagtatcac acggataagt
    40141 taccgaacat cacaccacat tcgttcagac atacttattg tacgaatatg gcaaatagag
    40201 gaatgaaccc aaacactctg caatatctca tgggacacgc taacataacc atgacacttg
    40261 gctattacgc acatggtaca tttcaatctg caaaagcaga actggaaaga ctggcttgtt
    40321 aatatcggag cttatattta ctactcattt actacttttg gttgcatttt catgctggta
    40381 aatgccagct tatgcaaggt atcttccaaa aagaaaatac cgataaagcc ctaaaatacg
    40441 ggatatatcg gcatttacca acttatgaaa agataatcag aaatttagta agtttttcta
    40501 tacaaaaaga cttaagttta taaaaagtta cattagtata cttaattcta aaattaaact
    40561 tatgttaata taaacaaacc tatattaacc taatttcaga attaaaaata aactattata
    40621 atttttttat aaac
//
LOCUS       DAEMHZ010000018        39384 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000018 DAEMHZ010000000
VERSION     DAEMHZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.018"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..783
                     /locus_tag="KPK72_000858"
     CDS             <1..783
                     /locus_tag="KPK72_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2488101.1"
                     /translation="KRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQKVSSFK
                     KTVEELLDEQGVKYNFEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFDTKHVN
                     DSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIKYGTKE
                     KVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTKVYIPK
                     LGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            893..1483
                     /locus_tag="KPK72_000859"
     CDS             893..1483
                     /locus_tag="KPK72_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG2488102.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNIREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGHKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            1549..3246
                     /locus_tag="KPK72_000860"
     CDS             1549..3246
                     /locus_tag="KPK72_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2488103.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLEESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDNMVEKLK"
     gene            3460..4299
                     /locus_tag="KPK72_000861"
     CDS             3460..4299
                     /locus_tag="KPK72_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG2488104.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            4816..6672
                     /locus_tag="KPK72_000862"
     CDS             4816..6672
                     /locus_tag="KPK72_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2488105.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    6755..6995
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    7061..7296
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7381..8598
                     /locus_tag="KPK72_000863"
     CDS             7381..8598
                     /locus_tag="KPK72_000863"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2488106.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            8715..9197
                     /gene="cdeM"
                     /locus_tag="KPK72_000864"
     CDS             8715..9197
                     /gene="cdeM"
                     /locus_tag="KPK72_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG2488107.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            9462..10727
                     /gene="hisD"
                     /locus_tag="KPK72_000865"
     CDS             9462..10727
                     /gene="hisD"
                     /locus_tag="KPK72_000865"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG2488108.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIMPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(10930..11682)
                     /locus_tag="KPK72_000866"
     CDS             complement(10930..11682)
                     /locus_tag="KPK72_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2488109.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(11800..12069)
                     /locus_tag="KPK72_000867"
     CDS             complement(11800..12069)
                     /locus_tag="KPK72_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488110.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            12544..13638
                     /locus_tag="KPK72_000868"
     CDS             12544..13638
                     /locus_tag="KPK72_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2488111.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            13640..14590
                     /locus_tag="KPK72_000869"
     CDS             13640..14590
                     /locus_tag="KPK72_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG2488112.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            14583..15767
                     /locus_tag="KPK72_000870"
     CDS             14583..15767
                     /locus_tag="KPK72_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488113.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            15780..17276
                     /locus_tag="KPK72_000871"
     CDS             15780..17276
                     /locus_tag="KPK72_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2488114.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            17299..18249
                     /locus_tag="KPK72_000872"
     CDS             17299..18249
                     /locus_tag="KPK72_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488115.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            18270..19232
                     /locus_tag="KPK72_000873"
     CDS             18270..19232
                     /locus_tag="KPK72_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2488116.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDEKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            19604..20236
                     /locus_tag="KPK72_000874"
     CDS             19604..20236
                     /locus_tag="KPK72_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2488117.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            20647..20739
                     /gene="kdpF"
                     /locus_tag="KPK72_000875"
     CDS             20647..20739
                     /gene="kdpF"
                     /locus_tag="KPK72_000875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG2488118.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            20780..22462
                     /gene="kdpA"
                     /locus_tag="KPK72_000876"
     CDS             20780..22462
                     /gene="kdpA"
                     /locus_tag="KPK72_000876"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG2488119.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            22487..24544
                     /gene="kdpB"
                     /locus_tag="KPK72_000877"
     CDS             22487..24544
                     /gene="kdpB"
                     /locus_tag="KPK72_000877"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG2488120.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            24563..25189
                     /locus_tag="KPK72_000878"
     CDS             24563..25189
                     /locus_tag="KPK72_000878"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG2488121.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLRKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            25426..26334
                     /gene="cysK"
                     /locus_tag="KPK72_000879"
     CDS             25426..26334
                     /gene="cysK"
                     /locus_tag="KPK72_000879"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG2488122.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLVGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            26361..26951
                     /gene="cysE"
                     /locus_tag="KPK72_000880"
     CDS             26361..26951
                     /gene="cysE"
                     /locus_tag="KPK72_000880"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG2488123.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            27111..27299
                     /locus_tag="KPK72_000881"
     CDS             27111..27299
                     /locus_tag="KPK72_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2488124.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(27353..28222)
                     /locus_tag="KPK72_000882"
     CDS             complement(27353..28222)
                     /locus_tag="KPK72_000882"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG2488125.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(28419..29663)
                     /locus_tag="KPK72_000883"
     CDS             complement(28419..29663)
                     /locus_tag="KPK72_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488126.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            29954..30265
                     /locus_tag="KPK72_000884"
     CDS             29954..30265
                     /locus_tag="KPK72_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2488127.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      30358..30450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            30550..31344
                     /gene="thiD"
                     /locus_tag="KPK72_000885"
     CDS             30550..31344
                     /gene="thiD"
                     /locus_tag="KPK72_000885"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG2488128.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            31368..32156
                     /gene="thiM"
                     /locus_tag="KPK72_000886"
     CDS             31368..32156
                     /gene="thiM"
                     /locus_tag="KPK72_000886"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG2488129.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFVSKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            32179..32811
                     /gene="thiE"
                     /locus_tag="KPK72_000887"
     CDS             32179..32811
                     /gene="thiE"
                     /locus_tag="KPK72_000887"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2488130.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            32950..33252
                     /locus_tag="KPK72_000888"
     CDS             32950..33252
                     /locus_tag="KPK72_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2488131.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLIGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(33318..34065)
                     /locus_tag="KPK72_000889"
                     /pseudo
     CDS             complement(33318..34065)
                     /locus_tag="KPK72_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34059..35021)
                     /locus_tag="KPK72_000890"
     CDS             complement(34059..35021)
                     /locus_tag="KPK72_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488132.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNSSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(35025..35483)
                     /locus_tag="KPK72_000891"
     CDS             complement(35025..35483)
                     /locus_tag="KPK72_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2488133.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPDRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            35839..36195
                     /locus_tag="KPK72_000892"
     CDS             35839..36195
                     /locus_tag="KPK72_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2488134.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            36267..36983
                     /locus_tag="KPK72_000893"
     CDS             36267..36983
                     /locus_tag="KPK72_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488135.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            36976..37800
                     /locus_tag="KPK72_000894"
     CDS             36976..37800
                     /locus_tag="KPK72_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488136.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            37812..39011
                     /locus_tag="KPK72_000895"
     CDS             37812..39011
                     /locus_tag="KPK72_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488137.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            39020..>39384
                     /locus_tag="KPK72_000896"
     CDS             39020..>39384
                     /locus_tag="KPK72_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488138.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRIN"
BASE COUNT        15368 a         4173 c         6872 g        12971 t
ORIGIN      
        1 aagaggataa taatatcatc tttattaagt gtatcaattt taatgggttt ggtaagtata
       61 tattctatat taaataaaga agacataatt ttaactgtta agggtcaaga gcaaaaagta
      121 tcttccttta agaaaacagt tgaggaactt ttggatgaac agggtgtaaa gtataatttt
      181 gaagataaga ttaatccaag tttagataca gaactaaaag atgatatgaa aataaaagtt
      241 gttaaggtaa ctaaaagtaa aaaagaagag attgaaaaaa ttccatttga tacaaagcat
      301 gtaaatgata gtaatttgtt aaaaggaaaa tctaaagttt atcaagaagg tcaagaagga
      361 gaaaaaaaac tagtctataa tttaacttac catgatggaa agttagtcaa aaaagtctta
      421 tcaaaagaag taatatctaa ggagccaact acaaaaatta taaaatatgg aactaaagaa
      481 aaagtactaa tagcatcaag aggagcaaat ataagaggag gcaaacatat gaaagtggtt
      541 gccactgcat atgcaggaga tacaataaca tctactggta caactccaag atggggtgtt
      601 attgcagttg acccacgtgt aataccatat ggaacaaaag tatatatacc taaactgggt
      661 atgacttttg tagcagaaga ttgtggaggt gcaattaaag gtaatagaat agacattttt
      721 atgaacagtg aaggaaaagc ttcaaattgg ggaaggaaaa gtatagatat atatctacat
      781 taaattagtg aatagaataa ctttacttga aggacaaaaa agtataaaat atgaattaac
      841 taaactgtta gattattctt aaaacataaa aatatatcta aggagggata agatgaaaat
      901 agtctgctta ggagacagtt taacgtatgg atttggagta tcacgaagta attcatggac
      961 aaacattgta aataaagaga ctcgattaga aatagtaaat aagggtataa atggtgatac
     1021 aacaagtggt atgttagcta ggtttaatga agatgttgta aaaaactctc ctgatatagt
     1081 ttttataatg ggtggcacca atgattttat tgctggtgcg ggaaatgaaa ttattaattc
     1141 taatataatg gctatggtgc atcagtcata ttcaaaaaat attataccta taataggtat
     1201 accattaaaa ccagatattc caaatattag ggaagattgg agttgtttta ctgattttaa
     1261 tatagtatct caaaacttag aatcatatag ttattggata aaaaaatttt gtatgacatt
     1321 taatactaat ttcgtagatt tttattcaga atataataaa aatatggaac tagaaggaca
     1381 taaaaaatta tattttgatg ggttacatcc aaccaaagag ggtcatagaa taatggcaga
     1441 catttttatt aattcaatta ataagtatat agaagagtta tagattacat taaaatgctt
     1501 gtttatgcgt cagttgcaat aaatacatac agtagagagg ggtttattat ggattatact
     1561 aaaacttatg aagagtggat aaagggttca tattttgatg aagatacaaa gctagagttg
     1621 gagaatataa aaaataatga aaaagaaata gaggatagat tctataagga tttagaattt
     1681 gggactgcgg gtcttagagg aataattgaa gcaggaacga atagaataaa taagtacaca
     1741 gttagaagag ctacttttgg attggcaaac tatatattag aaaacacaac aaaagaagaa
     1801 acaagtagag gagtagtaat agctcatgat aatagacaca agtctagaca attctgtata
     1861 gaatctgcaa atactttggc agcatgtggt ataaaagcat atatatttga tagtttgaga
     1921 actactccag aattatcttt tgctgttaga agcttaaatg caatcgctgg tatagtcata
     1981 acagcaagcc ataatcctcc agaatataat ggatataagg tatactggga agatggagca
     2041 caagttatgc cagagattgc aaatgcaata acagagaaag taaatagtat acatgattat
     2101 agtacaatac caaccttaac ggaagctaat aaaaatttgg tagttttgtt ggaagaatca
     2161 caagatacta aatttataga agcagtaaaa agccaaatta taagaaaaga tttagttaaa
     2221 aatgtaggta aaagctttaa gatagtttat acacctcttt gtggaacagg gaatgttcca
     2281 ataagaagag cattgaaaga agttggtttt gaaaatataa ttgtagttcc agaagaagaa
     2341 aatccagacc caaattttgc agggcttgat tatccaaatc cagaagagaa aaaagcttta
     2401 aatagaggta tattactcgc aaaagaaaaa ggagcagacc ttgtaatagc aactgaccca
     2461 gattgtgata gagtaggtgt agcagtaaaa actactacag gtgagtatgc tttacttaca
     2521 ggaaatcaaa ttggtggtat gctgacacat tatataatag aaggattaaa agaaaataat
     2581 aaattaaaag aaaatcctac aataataaaa accatagtta catcagaatt tggagcagat
     2641 atagctaaag caaataatgt tgatgtatta aatgttctta caggttttaa atttattggt
     2701 gaaaaaataa aattatttga acaaaataaa aatagaagtt atgtatttgg atatgaagaa
     2761 agttatggtt acttagttgg gactcatgct agagataaag atggtgtagt ttcttcattg
     2821 ttaatatctg aaatggcagc attttactat tcaaaaggta tgagtttata tgatggctta
     2881 atagaacttt ataaaaagta tggatttttc aaagaacaga ctatatcttt gactttaaaa
     2941 ggtatagaag gtgtagaaaa gataaaagaa ataatatctt attttagaga aaatcaaatt
     3001 gactatataa atagtattaa agtagttgat aaaaaagatt ataaaaatgg tatagataat
     3061 cttcctaaat ctgatgtttt aaaatacttt ttggaagatg aatcttgggt agctataaga
     3121 ccatctggaa cagaaccaaa acttaaattt tacatagctg taaaaggagc tagtgatatt
     3181 gaagcagata aaaagattca aggtttgaaa aagtatatag ataacatggt cgaaaaatta
     3241 aaataaaata ttattatttt ataagataat tttgtaataa tatttattta gctgaaaatt
     3301 ataatttact ttcattgagt agaatatgga aatgttagta gaattgttat tttcagcttt
     3361 tatttgtgtt atttttactt aaataattat attatagaat gaatctgagt ataattaata
     3421 ttgtataatg aaattaattt gatagaaaag gggatattta tgtttggtat agataagaaa
     3481 ttgattgaat atataattgc aaaatccaat tacagtgaat taagttcaaa gtcagcagaa
     3541 gtatctttct tcttaatgtt gtccatattt ccttttttaa tatttacaat aagttctata
     3601 gcttatatac cgattcttca tttaaataag tatatagctt tgtttagaaa tatgatgcca
     3661 gaaggtgctt ttgctgtgtt atcttcaatc atagtttctg caatagacaa tagaaacctg
     3721 aaatttttag cagtcagttt tgtacttact atgtggacat tttcaagagc agtaaaagct
     3781 cttattaaag gaatgaatag agcctataaa gttaaagaaa ctaggtcttt ttttaaaatt
     3841 ttatctatat catttttatt tacaataatg cttttggtcc ttattttttt atctatgata
     3901 tttttagttt acggggaaaa aataggttat tttattttta atcttgtagg attggatgag
     3961 atatttataa aaatatggga tattctaaga tacactgtag gtataataac aataatagtg
     4021 atatttacac ttttatataa atacactcca aataaaaagt taactattaa agaatctgcg
     4081 ccaggtgcta tttttgctac ttttgcttgg tttttagtat catttttata ttcttattat
     4141 acaaattact atgctaacta tgaagtaatc tatggaagta tagctgaaat aattgtactt
     4201 atgacctgga tgtattttag tagttggtct atcgttattg gctatgaggt aaattcaaga
     4261 ttgtatttta gaaagataag acatgaaatg ttaaagtaaa gtatttaaaa taaaagagag
     4321 tatttagtat tataaatgta attgaattaa taaagaaata ttgacattta agttaaaaag
     4381 tctagaattt ttctgggctt tttttatttt tagtataact tgacaaattt tatttaatgg
     4441 gatagtttta atttataatc atctttaaaa agatgactat tgataagtaa gagataaaat
     4501 tggtaagtat caaaaaatat actaaataaa aattatagtc ctaatgagtt aatttctgta
     4561 ttttcaaata atgcagtatt tagtcatgtg tttttatatg cgcctgtatt tctaattacc
     4621 aatatattag aataagctac tgtaattaga tttagaaaaa agcgacataa ataattataa
     4681 acaaataata aattacataa aattaaattt attcacaagt atatcttata aaatatgtta
     4741 taattaatag aaaattgtca tataattaat taagtagtat tattataaat aacattgaaa
     4801 ggtagtggtt agaatatgga tacccataat aaatatgtaa attttataaa aaatatacct
     4861 gtaccttttt tatattgtag aattgtaaag agacaagaag atatagaata tcgagtagaa
     4921 tatataagta agggtatggg caaagtatta caattagaag aaggtatttg tgataaaaat
     4981 atattagatg tattgcctgt atttaagtct aaaaaatatt tcaaagagtt gttctcaaat
     5041 gaagtggatt gtataaaaag gtacattccg acacttaaaa actggataaa tataaaaaaa
     5101 cagataatag gggattcata cataattttg tattttggta aaattgtatt tgattataga
     5161 cagataatcg attcctttga taaaaaggaa aaagtagctt atattaaaga cgaagaaggc
     5221 atatatatag attgcagtga aaatttaata ccaatattaa ataacaacat taaaacaacg
     5281 aaagatatct ttggtaaaaa tgatatagaa gtatggggag agaatacagg aaaattattt
     5341 agagatgatt atagagaagg agtatctagt aaaaaaagat ttttacaaaa tctttttgaa
     5401 tatgaagaaa cattttttat ggttgaaaaa tattttttgt atgatgaaga cgaactttta
     5461 ggtactatag ggatagtaga caatataata tatagtggtt acagtaatag aaattataac
     5521 tcaaaagatt taatgaagat gatagaacac tcaattccag agaatatgtt ttataaagat
     5581 gtgtatggaa attatattgg atttaattca ggttttttga atttagcttg tatgaataaa
     5641 gaagaattat tagggaagaa tagttataaa atatcagaag aagaagcttt aatagataag
     5701 atatttgaaa gtgataaggg tgtagttgaa aataaaaaag tagttacatt tgagttaaat
     5761 ataagtatga atgatgaaaa caagtgtatt gagattacaa aaaggccgtt ttttgatagc
     5821 tatggaagtg ttattggaat aattggaaca gcaagggata taagtagaag aaaaagatta
     5881 gaagaggaaa tggataaaac taggatggaa ttttttgcaa atttatccca tgaacttaga
     5941 acgcctataa acttaatatc atcttcatta caagtgatag aaaaaaaaga agcagattta
     6001 atagaatcaa atgatacttt aaaaagaaat ttaggtataa taaaacaaaa tggaaataga
     6061 atactgaggt tagtaaataa tgttatagat tttacaaaaa tgcaatcagg atatttagat
     6121 tttaaaccag aagaatctga tataattgca tttatagagg agatatgtat gtctgtagct
     6181 gattttgcaa gccaaaataa tatacaactt acatttgata cagaaataga agaattttca
     6241 atgttatttg attcagaaaa attggaaaga ataatattaa atttactttc taatggaatt
     6301 aagtacaata agaaagatgg aaaaataaat atatttttat atgtgaaaga taatgtattt
     6361 aatatgaaaa tatctgatag tggaatagga ataccaaaag aaaaaataga taaaatattt
     6421 aataggtttg aacagattga caatgaactt tcttacagag ttaaaggaag tggtattgga
     6481 ctttcattag tgaaatcttt ggttgagttg catgagggaa gtatatcttt aaaaagtcaa
     6541 cttggtattg gaagtgaatt tatagtttca ttgcctgtta gaagtaaaaa taatattgaa
     6601 aaatataatc ataaaagaga aatcagcaat gaactaagta aaaaattaga aatagagttt
     6661 tcggatttat aagtttaaat ataaaaaata aaattttaaa aaagtattga caattgattt
     6721 agaagggttt aaaataaatt acaacataat aaataaaaag gcgatgaata ggaagagtaa
     6781 atctaattcg attaccagag agaagagtcc attggctgga agacttttta aattagaggg
     6841 attgaaaact acctaggagc tactttctga agtcatctgc attagtagat tttagtaaga
     6901 atagtcgtta gctgcgttaa agtgtaaagt gagttttcat gacatatgaa aactaaatta
     6961 ggtggaaccg cgggaataat actctcgtcc ttatgttata aggatgagag ttttttgtta
     7021 tttaaaaata taaacagtaa tcataataat tatagaaaat ataaaagcga tgaataggaa
     7081 gagtaaattt aattcgacta tcagagagga aagttcacag gctgaaagac tttctaagtt
     7141 tgagaaattg aaaactacct aggagctgtg gtctgaagtt actttaaaaa gttttagtaa
     7201 gactaaccgt taactgcgtt aaagtgtaaa gtggatttta tcaataaatg ataaaatcaa
     7261 attgggtgga accgcgagta tagactcgtc cctttatata gggatgagtc tatttttatt
     7321 gcaataaaag attgaaaata aaaaaaaatc ggaaaattat aaatttagga ggaaaataaa
     7381 atggagaata aagtgaaaat tggagtacta ggatatggag ttgtaggaag tggacttata
     7441 gatataatag ataataataa agaaaaaaga aatatagaaa tagttggaat tttagttaat
     7501 aatttggaaa aacataagga taaaaaatat tctaatataa taactaataa tatagatgat
     7561 atatttaata aagatataga tatacttgtt gaagtcatgg gtggtttaga accatcatta
     7621 tcttatataa aaaaagcatt aaacaataaa attcatgttg ttacagcaaa taaagatttg
     7681 ttagctgaat gtggagatga gcttgcaaaa ttagcaagtg aaaataaagt aagtattaaa
     7741 tttgaagctt cagttgcagg aggtatacca gtattaaaac caataataga atcactagaa
     7801 ggaaataata tagatagtat aaatgcaatt ttaaatggaa ctactaattt tatattatct
     7861 aaaatgtatg atgagaatct atcttatgat atggcattaa gacaagcaca agaattaggt
     7921 tttgctgaag caaatccaga gtcagatgta ttagggtatg atgctgctag aaaactatca
     7981 atattatcaa ctctagctta tgacaataga gtatattgga aagatgtgta tttagaagga
     8041 attacagata tagatgaaaa agatattgaa tatgcaaaaa aattaaattg taaaataaaa
     8101 ttgattggtc aaagtaagta tgaaaatgat aaagtaagtg cttttgttag acctgtttta
     8161 gtagaaaaag ataatatcct tgctagaata gacaatgagt tcaatgctgt catagtgaat
     8221 ggagattcag taggagaagt ttcttttgta ggtaaaggag ctggaagttt agcaacagga
     8281 agtgcggttt attcagatgt tatagatata atagataata gagtttcaag tatagattct
     8341 tttactaaag ataaaataca agttaataaa atagttagag aaaagtgtgg agcacttctt
     8401 agatttaaaa aatgtaataa agatgaaatt ctaaatatag ttgagaattg tttagtaaaa
     8461 ttcgatattt tgaatgatga tgatgaactt gctattatgg tttatgctga ttctgaatat
     8521 gaaataaaca acagtttatg tctaataaaa gataaaggat actgcgaaaa aatgaataaa
     8581 atgttgaaaa taagttaaaa aagaaaaaat ataagtttat atgtaacata ttttgacgat
     8641 tttaatacta tatagtatga gatgaaacaa aacatctcat aaaaaaatta aaattaaagg
     8701 gagagatatg ttatatggaa aataaaaaat gttattcaga agattggtat gaaagaggag
     8761 aatctacagc taaatggttc caaaatgata gagaagaata tgaaagagaa gcatatgatg
     8821 aagatagaga aagaagaggt tcaaactgtg gatgttcaga ttcaggagaa aatagaccta
     8881 gaaactgtga aagatttaga agagaagctg agataagaga aagagaagca agagaagcat
     8941 tttgtgaatc ttcagagaaa aagaaagagg cattagcata tgaatgtgaa gctagaaaat
     9001 tatgggaaga agcagaaaaa tactgggatg aatattcaaa atacaactat aaaggaatcg
     9061 aatatttagc agaagctgct agattatttg atgaaggtat ggaatgtgaa gctagaagaa
     9121 atggaaataa tggaggaaac aataataatt gttgccataa atgccataaa tgtaattgta
     9181 actgctgtag aaaataaaaa aattaattga caaatcaata gttaaaatat atcatattat
     9241 gtataataaa gagaaaattc agaaaaaaat aatgattaga agaaatataa gtatatttat
     9301 tagacaaata gtgctcagtt agtgaggtga gtacaatatg attaaactcc catcctctag
     9361 cctttgatag agggtgggag tttttattta tttatatatc ttaaatgagg ataatattgt
     9421 aaaataaatg agtatgagat tgtaaaatta ggaggtaaaa aatgaaaagt attaaaacat
     9481 caagtatgaa agaaaaggta gatgaaaaga tattaagaga gcaagtaagt tctataatag
     9541 aagatataag aaataataag gatgttgcac ttaaaaaata taatgagaag tttgatagaa
     9601 atacaagaga tgaatttaga attactaagg aagaaataaa ggaagcttat aagcatgtag
     9661 atgacgaatt tataaataac ttaaagattg ctgccaaaaa tataagagaa tttgctaaag
     9721 ctcaaaaact aagttttgaa aacccttttg aaaaagaaat ttatccaggt gtaatcttag
     9781 gtcaaactaa tattccaata gagtcatgtc ttgcatatgt tccaggaggt cagtatccat
     9841 tgttttcaac agctcttatg cttataatgc ctgcaaaagt agcaggagta aaaagaatag
     9901 tggcatgttc accaactatg aaaaatactg aaaagataaa tcctaaaaca ttggtagcta
     9961 tggatatagc aggtgctgat gaaatatatg caactggagg agttcaagct atagcagcat
    10021 ttacatatgg aacagaaaag attaatccag ttgacataat tgtaggacca ggaaataagt
    10081 ttgtaactga agctaaaaga caatgttatg gacaagtagg gattgatttt gtcgctgggc
    10141 caagtgaagt tcttataata gcggatgaaa cttcaaatcc agtttatatt gcagctgatt
    10201 tattagcaca atgtgagcat gatttaaatg caagaggaat attattgaca aatagcttgg
    10261 aagtagcaca aaaagttgaa aaaaatatag aaagtatgct aaaagatttg ccaacaaagg
    10321 acatagcata tagttcatgg gaaaataatg gagagataat attggttgat gatatggaag
    10381 aagccattaa aataagtaat ttttatgcac cagaacattt agaagttgca gtaaatgagt
    10441 ataatgatat ttgtgataga ttaactaact atggttcttt atttattgga aatctttctg
    10501 ctgaggtgtt tggtgactat gtttcaggta ccaatcatac attaccaaca cttaaagcat
    10561 caagatatac tggtggtgtt tgggtcggaa cttttatcaa aacttgtaca aagcagatat
    10621 ttaatgaaaa agctatacaa tcattagcac ctgtagctga aaagctggca aaagaagagg
    10681 gattatatgc acatgcaaag gctgctgagg ttaggtttaa aaagtagtaa aaaattttat
    10741 tttggcagtc gagatttagt attagactga gataaggaaa acgctgagaa cccgaataaa
    10801 tactatattt tgtgggcctc ggcgtttctt ttatatctgt atataatagg ataatactac
    10861 ctgtgtgttt aaatactaac ttaatatttc ttttctaaat aatgtttatg aaatagttaa
    10921 tatttttagt taatagttga aaaaaacttt tgctaactgt gcaaattcat ctgcatttgt
    10981 caatatagca gggtgtcctc cctgactaaa aatgtgaata cttgcttttg aaataaaagt
    11041 tgccatttgt ttgtactctt cttcaagatt atgacgacac atttcgtctt ccttacttcc
    11101 tgtaaataaa attggcattt ctaatttaca caattcttta tgaaacaaag ggcgtttctc
    11161 gtttgcacat tgcaatagag cttttgtatc aagattaaca acattctccc aatcttttcc
    11221 ctgacaccat tcatagaatt ttcgtgcttg aatatcttgc tttgattttt cccttccagt
    11281 gattagatta tctatgaagt tatcattaag agtacgtcca tcaaagctat ctgctataat
    11341 tgcttcaact aaatcgggac gttctaatcc agtattaaca gctgcccatg ctcctccact
    11401 tgttccaatt aagccaactt taccacaatt caaatgttca atcagtgcta ttgtctgtaa
    11461 ggcttcatca tgccatatgt caggagaaaa ttgagaaact ctatcagact gtccattacc
    11521 taaaaaatca attaagatac atctaaaatt ttctacatat aatggcacaa gtgctttaaa
    11581 catattagaa gaaccagtat taccatgcag gaaaattatc ggtttacctt gtccatattc
    11641 tttataaaaa caagaattac cttgatactg aaaatatgac atatattttc ctccttttta
    11701 tttgttaagt taattataac aatataggta tgaatgaaaa acctcatgtt ctttcttaga
    11761 ataaaaatgt gccacgcata aaacgtgaca catttctaat tataaatttt gctgtttaag
    11821 tattctttgt atgcttttct ctgtcaaaaa gtatttctct gataactgct tgatactcat
    11881 tccattttgg aaatttatac agatttgaga attacgagaa gctaagactt gtttggtgtt
    11941 tgtgttttca ccccaatgct ttttgtgctc ttgctttttg ggaatataaa tcactttacc
    12001 atctatatat tcttgcactt gctcaatcag ttctttaggc agtaagtcaa tcgaattttg
    12061 ataactcatt ttgcacctcc taaataatta ttctttagga ttagcaaagg ctactgccac
    12121 tcacaattaa aaataaaatt ttttattatg tacaatagcc tttgctatgc ataagttcca
    12181 attcccataa actaaatact cctttctggt ttgaaaaatt catctgaaca ttgatttcgc
    12241 ttttaagata gagttcaatg acaaatttac atttttatta tatatattca gatagataat
    12301 tgcaactgtc tttgtatgag gatattttat tatagtcaat actataatat gtatttatat
    12361 catcaaacta atatgaacag ttgaaaggaa tatgaacaat aaaacttagt caggaagaat
    12421 aattatgaag cagacaattg agaaatttga ttttaaagtt ttattgaaaa tttattgaca
    12481 aatttaactt gtaaatatta tactaaactt aataaataaa gtttataaag taaggtgttg
    12541 tctatgaaaa atatagttgg aaaaccgcaa acgataaaaa aagtaaatga agatttaata
    12601 aaaaacatta taaaaaatga aggtcctata acaaaaccag aaatttcaag cattactgaa
    12661 ttgagtttag ctacagttaa taagattgta gagcaattag ctttgaaaaa tgaagttaaa
    12721 gtaagtggac taagtgaatc tactggagga agaagagctc aattatttga aataaatgca
    12781 aacttagaac acataatagc tttatattat tatagaaatt gttgtatagg ggtagttgcc
    12841 aatttattag gtgaaataat ttatcaagaa gaatttaata taagaatgga tacgtatgaa
    12901 aacgttaacc aagatacttt ttttgttata gatactctaa taaaaaagga aaaaggaaac
    12961 aatataaggg ctattggaat tggtgtccca ggagttgtaa aaaaaggtgt agttaaaaat
    13021 ataccaacta taaaaagttg ggaaggtata aatttagaaa atttaataaa gagtaaatac
    13081 aatactaaag tgtttataga aaatgataca aatattacta ctattgggat ttatcaaaaa
    13141 cagtataaag acaaatataa aaatatggct cttatgtatt tagaagatgg tattggttca
    13201 ggtatagtta taaatggtga attgtattta ggttctacaa attttgctgg agagttaagt
    13261 tatctaaata tagaatttgg taagtataac tctaacaact taaatcttga agattatatt
    13321 atgagtttaa gaaaaagata tttagaaagt aaagataata aatttaagaa agaaatttta
    13381 tctataatat caaactttat aaggactctt gtatgtgtcc taaatccaca agctatgatt
    13441 atacagtgta gtatattaac caagagtgac atggagttta tagaaaaaga tgtaagcaat
    13501 aatgttggag aagaaaattt tcctaaatta ataaaagtag agtcattgag agaaggaagt
    13561 ataaatggag taattagtat gtgtttaaaa gaaactgact atcaatattc cttatctaat
    13621 aaaaaaggag gatattaatg tgaaatattt ggtttcaata gatggagggg gaacaaaaac
    13681 taaattttgt gtaagtgact tagatggaaa catacttaaa gaacatacca ctggttcaac
    13741 taattataag tcagttggaa ttaaaaaaac atatgaaaat attaataatg gatttaaaaa
    13801 aatattaaaa gatttatata tcgattatga cgatattgag tacactgtat ttggtatatc
    13861 tggttgtgat tctcctaatg attataagat tataatggat gaaatattaa aaattgggat
    13921 taacaaagaa aaaatatatc tggcaaatga tgcagtttta gctttttatg cacaagcgga
    13981 ttcccctggt ttagtaattg tggctgggac aggttcaatt atactgggaa ttaaagaaga
    14041 tggagaaata tatagagttg gtggatgggg gtataatttt agtgatttgg gttctggata
    14101 tgatataggg agaaaattat taaagaaagt attgctttat tgtgatgaat gtcatgagta
    14161 tagtgattta tttagttgtg tcttggattt ctttggagca aatagttttg aacaacttgc
    14221 atatatgatt actgatataa acaataatgt tgaaatagca aacttagctt cattagtgat
    14281 tgattgtgcc gaacaaggtg ataaattagc tattgaaata ttaagagaat catcaactga
    14341 actatctaaa ttagctcaag taatattgag aaaaatatct aatccaatga aaatggataa
    14401 aagcgaaata aatatagttt tgtctggtgg tacattaaat aaaactgtat atgctcaaat
    14461 gttaatgaat aatttaaaaa cagaaaatac agataaaaat ttaaaattta tatctcaaga
    14521 aaatcagcca gtgtatgggg ggataagact tgctatggca ttagctaata ggagggtcaa
    14581 gcatggatag aaaaaaggta atagtacaaa tttttactgg aggttttaag gctaaagaaa
    14641 taaagtttaa tgatataaaa tgtaaactac tagaattatt ggactgtatt gatatagaaa
    14701 aggttatcat gggatggagt gtagatgaag aattatatgt agaaactaaa aaactattaa
    14761 ataaatataa tgttgaacta tatctttggt tgccatgttt ttctgaaata ggattacttg
    14821 aaaaaagtaa tttactaatt gattatatgg ggaaggaagt caagggctat gctttacaag
    14881 aggatgaaaa ttttgagttt tattgcccaa ataataagga aaatataaaa aatataaaga
    14941 aaatatatag tacttatttt agtgatatta aatttgatgg aattttttta gataaaataa
    15001 ggtatggttc tttttcgaat aaactaaatg gtgtttttaa ttgtttttgt aaagattgtt
    15061 taaatatata tgaaaaaaat aatattgata aagagtcgtt aatgagagaa atgaataatg
    15121 tgtctaaggg agttatagat aatgaagata ctccatttgg tataacagaa tacattaatg
    15181 gaaaatacaa atttaaaaat aaaatatggg aagatttttt tgaaacaaaa aatcaaaata
    15241 tatacaaggc attattagag ataagtagtt attttaaaag taagggattg aaaataggta
    15301 tagatacttt ttctcctttt atttcttact ttacaggtca agatttagaa aaattacaag
    15361 aagtagcaga ttttataaaa ccaatgatgt atagaactac aaaagctcca gctggtttac
    15421 catttgaatt tgaaaggttt attgatgaat ccatacataa gaataaagag attggaaaaa
    15481 gaaatttctt agatatttta ggaattaagg agtatgaagg agatgtatac cctatagaat
    15541 ttgtactaga ggaaatcgat tttatgacaa gaaatcttaa taaagatata tatgtaggag
    15601 ttgaaataaa taaaaaggaa attatagctc ctgttacacc taagtacata agtgaaaata
    15661 tttcaaatat aaataagaca acagtaaaag gatatgtttt atcttgggat ttgttgtcag
    15721 caccaaaaga aaatattgat gaaattataa aatggtttcg gaattagtga ggtgataaag
    15781 tgagtaagat attggaaatg caacaaatat ataaaagttt tggaaaggta gatgttttaa
    15841 aagggataaa ttttgagtta gaaaagggag aagtaagagc tcttttggga gctaatggag
    15901 caggaaaatc tacacttatt aaaattttag gaggagttca ttcccaaact aaaggtaaag
    15961 taattttgac tggtaatgaa ataatgttta aaaatccaca tcattctaaa gaaaatggta
    16021 ttagtataat ttatcaagaa ttaagcttga taccaacgct tacagtactt gaaaatattt
    16081 ttcttggtag agaaatttta aaaggaagtg tgtttttaaa caagaaagac atgcttgagg
    16141 aatatatgaa aatttgtgag gattttgatt ttaatataga cccacatata gtagtttcta
    16201 atttaagtgt agcaaaccaa caaatggttg agataatgaa agctgtttca tgtaattctg
    16261 aaattatcat aatggatgaa cctacaacat cacttactaa taatgaaaaa gatgggctat
    16321 ttaaaataat aagtagacta aagtctaaag gtaaaacaat agtctatatt tctcacatat
    16381 tagaggaagt ttttaaagta tgtgatagtg caagtataat gaaaaatgga attatgattg
    16441 gaacttatga tgtagaggat ttaactaaat taaagataac tcaacttatg acaggaaatg
    16501 ataatcacaa acttattgag aagaaggatt atacatttgc aaaatatgaa gagccaccag
    16561 tactagaagt aaagaatata agctggtcta acaaaataaa aaatgtttca tttaaagttt
    16621 atagaggcga agttgtaggt cttgcaggtc ttgtaggttc aagaagaact gaaattataa
    16681 atttgatatt tggtatagat aataaagata gtggagacat ttttatcaat ggtaaaaaag
    16741 tagacataac ttctcctaag gttgctataa aaaacaaaat aggattgatt cctgaagata
    16801 gaaaaaatct aggattaatt ttagaacaag ctatatacac aaactcatca tcagtacaaa
    16861 ttgataaatt taagaaattc ttgtttatag atagaaataa agaaattgag ttttctaaaa
    16921 aaggtgttaa ggaattagga ataaaagtta gtgatgtaaa gcaagaagta aaagagctta
    16981 gtggtggaaa tcaacaaaaa gtagtagttt ctaagtggtt agagaaagat ttagatttaa
    17041 taatatatga tgagccgaca aagggaatag atattgctgc aaaagaagat attttcaata
    17101 caatcaaaga attttcaagt aaaggaatag gagtaatatt tatatcttct gatttagaag
    17161 aagtaataag agtaagtgat agaattttag taactagaga aggttctatt gtagatgatt
    17221 taaaaaatga gaatatacag gttcaagata taatgaacag tatatttaat gtataaaaaa
    17281 tgaaaatgga ggactgagat gaagaaagct tttgagaagt taaatttaag agattatgga
    17341 gtattattag gttttttagc tttatgtata gctataagta ttgcaactcc atcattttta
    17401 ggtaaacaaa atatacttaa tcttttacga caatcttcta taattggaat aatttcagca
    17461 ggtatgactt ttgttataat atctggaaac tttgatatat cagttggagc agtagcagct
    17521 cttgcaggtg ctataacaat gaaatttgcg actggtggaa ctaatttgtt tgcatccatg
    17581 ctcttaggtg tagcgatttg tgcagtaata ggattaatca atggtatagt agttgcaaag
    17641 ataaatgtac catcattaat agccactatg gctatggtca ctattgtaag aggattgctg
    17701 ttgatgctta caggaggata tccaattact gaaaatatac caatacttga ttacattgga
    17761 aatggatatt tgtttaatat accaatacca gtagtaattt tctttatagt ggtgttagct
    17821 tcatttattg tacttaataa aactaagttt ggtagatatg tttattctgt aggagggaat
    17881 caagaagcaa gtaaacttaa tggtataaat gtagattctc acaaagtgaa agtgtttatt
    17941 attaatgctg tacttgcagg aatagctgga atcgtactaa caggaagact tgggactgca
    18001 actgcgattg caggtgaagg atatgatatg gatgcaattg catctgtagt aataggaggt
    18061 acatcagtgg caggaggttc tggttctgta cttaaaactg ttataggagt attacttatg
    18121 agtgttataa acaacagttt taatcttctt ggaatagatg tatatttcca gtacatattt
    18181 aaaggattaa taattttagc agcagttgga tttgattctt atagcaagaa aaaacttgct
    18241 tcaagataaa aaataagggg gattttttta tgaaaaaatt aatgaaacat ttagcattgt
    18301 tgctatcact tgttatgata tttggtttag ttggttgttc aaatggtggt gacaaagaaa
    18361 aaaatggaga aaagaaaata gcagtattac taccgggttc aacaggttac tttgtagcaa
    18421 ctaaacaagg tatagatgaa aaatctaaag aattaggtgt aagcgtggaa tatgcagatg
    18481 ctcaatggga tgctagtaaa caattatcac aagctgaaga ttttatggct aaaggggtag
    18541 acatgattgc attatgtgga gttgattctg ctgtaagtga gagaatagtt aaagctgcta
    18601 atgattcaga cgttcctata gtagcattta caaattctat aggaagtaac ccaactggag
    18661 aatttaaagg tttagttaca tatataggac aaaatgagga agaaacagga gcattaactg
    18721 gaaaaattgc taaaaactta ttaggagaaa ctggtgggaa agctgtatta atagaaggag
    18781 taccaggaac tactccacaa gtattaagaa aaaaaggtct tgaaaaagaa ttaaaagata
    18841 gtaatataga aatagtttat aatcaaactt caaattggga gaaagagcaa gctttaaaag
    18901 taacagaaga tttaattcaa aagaaaactg attttaatgt tataatatgt caagatgata
    18961 attctgctac aggtgcaggt caagctttaa aagatgctgg tcttaaggat aaagttaaag
    19021 tcataggttt gggtggaagt aaagacggat taaaagctat aactgatggg ttaatagatg
    19081 gtacaactta tatgtctgct gtagaagaag gtggattagt catagaaaag gcatctaaat
    19141 tccttaaagg tgaaaaaata gaacctgtta cacaaattaa acaagttgaa gttaacaaag
    19201 ataatatttc agagttcaag ggtgaatggt aaattgtcat agataataaa taagattttt
    19261 agagttactt tagaaactat ataaagtaac tcttttttta atttaattta gtgataaatt
    19321 attgaatagt atttctaaac aaataaaaaa ttaatataaa caaagactta attatactaa
    19381 attataagta aaactaatat aaacttatgg aaaaaaggaa aacgtctaaa gtattggaat
    19441 tactatagtg taggtgtggt attaaaaaaa cataaaaaaa cataaaaaaa ttgttgaaaa
    19501 aaatatagaa attttgtata attaaagatt tgaaaagtgc ggtatacaaa gccaattctt
    19561 ttgtatataa tatatataaa ttcagaaaaa ggaagggata tgtatggcca ggcggttatt
    19621 tttagaaagg attggactta tatttttagg ttcagcaata ttagcatttg gagtttataa
    19681 tttttattat ttaaacaaca taacagaagg tggagtgtta ggtattttac tactgttaaa
    19741 aaatttattc aatatacaac cagcaatagc aaatgttgta atagatggtt tactactttt
    19801 agtaggatat aaatttttcg gtaaaaaatt ccttatatat tcaatagtag cttctataac
    19861 tttttcagtg ttatatgact tatttgaagc tataggacct ctagtaccac aatcacaaaa
    19921 tatgctttta tcaactattt tggcaggtgt aactgttgga actggtgtag gtatagtagt
    19981 aaaagcaggt tgtgcatcag gaggagacga tgctttagca ttagtaattt ctaagactac
    20041 atctttgaac atagggcaag tttatttagc aacagatgtt attgtattgt tgttatcatt
    20101 attttatcta tcagcgtttg atatctttta ttctttgatt gctgttacaa ttagtggcaa
    20161 ggtaattgat tttatttatt atcatggtaa aagtttggat atggatataa gtaatgatat
    20221 agttccagaa tgttaatata aattaaataa ctttagattt tattaggggt ggcagatttg
    20281 ccaccttttt tgtgtctaaa tctttataga aagtataatc ccatctaggt ttttgcaaat
    20341 ctcaaatata gaattatgtt ggaatgactg ggaatacgat tgctagatgt gcattttaaa
    20401 aacccaattt tatatttatg aaatactaat cattaatgga tgtttcttaa tcctatcttt
    20461 atacctaaaa tgataagata aatgaagtag caatacaaaa ctaaaatttg taaaagagat
    20521 taaattaaat ggaggagcta aaatgttgga tatagttatg atagcattgt tattcatagg
    20581 atttgtttcc atgaagttac ttgctgattg gtgtgggaaa caaactgaaa aaaaataatg
    20641 taatgtatgg agggattacc tatgtggttt ctggcagttg taattataat gttaataata
    20701 tatttggtat atgcgcttat taatcctgaa aaattttaaa cagtataagg ctaataagta
    20761 agttgaattg gggggaacaa tttttatggt gcagatagta atagtgttag cgatttttat
    20821 gattttagta atacctatgg gcaagtattt atatcatata gctactaatc aaaaaacatt
    20881 tggagataga ctatttgata aagtggacaa ttttatatat aaggtatgta gtatagataa
    20941 gaaaaaagaa atgaactgga aacagtatgc attagcttta ttatttacaa atgcagttat
    21001 ggtatttata ggatatatca tacttagaac tcaaagtatg catattttta atccaagtgg
    21061 aattaaaagt atggaacaag gactatcatt taatacaata ataagtttta tgacaaatac
    21121 aaatcttcaa cattattcag gtgaatcagg actttcatac tttagtcaga tgacagtgat
    21181 aatatatatg atgtttacat cagctgcaac aggatatgcg gctgctatgg catttgtaag
    21241 aggtctagtc ggtaaaaaga aaacattggg aaatttttat gtagatttaa ttaggataac
    21301 aacaagagta cttttaccag gagctttaat tattgggctt atattagtta cacaaggagt
    21361 accacaaact tttgctggga cagaaacagt gactactata gaaggaaagc ttcaagatat
    21421 agctagaggt cctgtggcag ctcttgaatc aataaaacat ttaggaacaa atggtggtgg
    21481 attctttggt tcaaattcat cacacccatt tgaaaatcca acaataatat caaatatagt
    21541 agaaatatta tctatgatga tattaccagg agcttgtgtt gttgcatttg gtcacatgat
    21601 aaagaataaa aaacaaggtt gggttgtgtt tggcgcaatg tcaatcatat ttttaatagg
    21661 tctagtagtg tgctttaaag cagaaagtgc aggaaatcct attttatcac aattagggct
    21721 taatcaaagt atgggaagta tggaaggtaa ggaagttaga tttggtatag cacaatcatc
    21781 attattcaca acagtgacta cttcctttac gacaggtaca gttaataata tgcatgatac
    21841 actgactcct ttaggaggat tagtaccact tttaaatatg atgctaaatg tagtatttgg
    21901 tggtaaaggc gtaggtctga tgaatatgtt gatgtacgct ataatagcag tattcttatg
    21961 tggattaatg gtaggaagaa caccagagtt tttaactaag aaaatcgaag gaaaagaaat
    22021 gaagcttatt gctttactta ttatattaca tccactttta atactaatgt tctctggtct
    22081 ttcagtagca ataccagcag gacttgaagg gatttcaaat cctggattcc atggattatc
    22141 acaggtattg tatgagtttg catcttcagc agccaataat ggttcaggat ttgaaggtct
    22201 aggagataac actatgtttt ggaatataac aacaggtata gttatgttct ttggaagata
    22261 tgtgtctatt atagtactac tagcaatatc aagtttgtta gcatctaaaa aagctgtaaa
    22321 tgaaagtata ggaacactta gaacggataa ttttacattt acaatagttt tagtacttgt
    22381 tgttttaatt gttggtgctt taacattttt cccagcctta gcgcttggac ctatttcgga
    22441 gcatctagtg ttgtggcact aaaaaattgg gagggagtaa agttttatgg aaactaaaaa
    22501 aacaaagttt attacaaaag atattttcaa aacttccatt atagaagctt ttaaaaaatt
    22561 aaatcctaaa tatatgatga aaaatccagt tatgtttgtt gtagaaattg gatttatagt
    22621 aacattgata cttacaatag tacctagttt gtttggtgat gaaggcaata acttaagagc
    22681 ttataatggg attgtaacaa taatcttatt tataacagtc ctttttgcaa attttgctga
    22741 agctgtagct gaaggaagag gtaaagctca agcagataca cttaagaaaa ctagaaaaga
    22801 tacagtagca agggttatag atacgttagg aaatgaaaaa atgataagtg caagtgagct
    22861 aaaacttggt gatattgttt tagtaaatgc tggagaagtt atcccaaatg atggagaagt
    22921 tatagagggt atagcttctg tggatgagtc agcaataact ggagaatctg cacctgtcat
    22981 gagagaatca ggtggagatt tcgcatctgt tacaggtggt actacagttg taagtgactg
    23041 gttaaagata agaataacaa caaaaccagg agaatcgttc ttagataaaa tgatagctct
    23101 tgtagaaggt gcttcaagac aaaaaacgcc aaatgagatt gctttaaata ctttgttagt
    23161 agggttaaca cttatattta tggttgtttt aatatctctt tatccaatgg caaactatgc
    23221 aggtgtaaag attccaattt ctactatgat agctcttttg gtatgtttaa taccaactac
    23281 tataggtgga ttgttgtcag ctattggtat tgcaggtatg gatagagtaa ctagatttaa
    23341 tgtaattgca atgtcaggaa aagcagtaga agcttgtgga gacgttgata ctatgatttt
    23401 ggataagaca ggtacgatta cttatgggaa tagacttgca tcagaattta tagcagttgg
    23461 aaatgcagat tcaaaagatt taataaatta ttctgttatg tgttcattaa aagatgatac
    23521 accagaagga aaatctatag ttgatttagg tatgaaatta ggttcaacag gtaagacaaa
    23581 agaggcagag gaagctgagt ttgtagaatt ttctgcacaa actagaatga gtggtattaa
    23641 cttagctgat ggtagagcta taagaaaagg tgcttatgat agcataataa aaagagtaaa
    23701 agaatctggt ggtacagtgc cagaagattt agaagaaaat gtaaatcgag tagctaaact
    23761 aggaggaaca cctttagtag tgtgtgtaga taatgagata tatggaatta tctacttaaa
    23821 agatacagta aaatcagggc ttgtagaaag atttgctaga cttcgagaga taggaataaa
    23881 gacaataatg tgtacagggg ataatccctt gacagcagct acaattgcaa gagaagctgg
    23941 ggtagatgga tttattgcag aatgtaaacc agaagataaa atagatgcaa taaaaaaaga
    24001 acaattagag ggtaaaatag ttgcaatgac aggtgatgga acaaatgatg ctcctgcact
    24061 tgctcaggca gatgtaggtc ttgcaatgaa tagtggtaca acatctgcaa aagaagcagc
    24121 aaatatggta gatttagatt cagacccaac taagatattg gaagttgttg aaataggaaa
    24181 acagctttta ataactcgtg gggctttgac aacatttagt atagcaaatg atgttgcaaa
    24241 atattttgcg attataccag ctatatttac tcttgcaata cctcaaatgg agatattaaa
    24301 tgtaatgaaa ctttctacgt catttagtgc gatattatca gctttaatat ttaatgctat
    24361 aattatccca tgtttaatac ctcttgcaat gaagggagta aaatatagac ctatgaaatc
    24421 acaagctatg ttaatgagaa atatgcttat atttggtctt ggtggagtta tagtaccatt
    24481 tttaggaata aaattaattg atttattgat tacaccttta gtaatggttc ttaatttagg
    24541 ttaataaatg gaggatataa aaatgaaaac attaaagcca gcattacttg taagtattgt
    24601 attgttagtt gtttgtggac ttgtatatcc acttgttttg acaggtgtaa gtcaagttgc
    24661 atttaaagat aaagctaatg gaagtatgat agaagtaaat ggtgtaaaag taggttcaga
    24721 acttattggt caaagcttta cagatgcgag attctttaag ggaagagtat catcagtaaa
    24781 ctataatact tatacaaaag aagatttagt accagataaa gatggaaatg catcttatgg
    24841 aggtgtatca tcagggtctt ttaattatgg agctacaaat ccagaacttc atgatagagt
    24901 acaaaaagat atagaaaagt ttttaaagga taatccaact gtcaaaagag aggatatacc
    24961 aacagattta cttacagcat caggttcagg acttgaccct aatataagtc cagcctctgc
    25021 aaaaattcag ataccagcta tagcaaaagc gtctggaata tcagaaagta aattacgaaa
    25081 aattgtagat gataatactt ccaaaaaact ttttggagtt ttaggtgaag atagagttaa
    25141 tgtactaaag gttaatgtag aggtcgcaaa aatattaggt cttatatagt attttgtgga
    25201 attttagcaa caaaatatta cgcaagattt aatttaaagg ttaaatttta aagagtgaaa
    25261 atcgataatg attttcactt ttttatttga atattactaa tatttggaat aaagtgattg
    25321 taagtataaa atccaaaaaa aggttgcatt atcaaaatga taatactata atatatttat
    25381 cgttatcaat ttgataatag atttggacat ataggaggaa atagtatgtt atataataac
    25441 gcattagagt tgataggaaa cacacctgta gtaaggctga ataatttagg ctgtactaat
    25501 gggtgttcta acatatatgt aaaattagaa aaagtaaatc cagcaggaag tataaaagat
    25561 agagctgttt atgaaatgtt agagggctta gagcaaagag gagaattaaa aaagggagat
    25621 gtactagttg aagcaactag tggaaatact ggtattgcat tatctatgat aggaaaatta
    25681 aaaggatatg aagtagtaat cgtaatgccc gaaacaatga gcgttgagag aagagattta
    25741 atgaaggctt atggagcaaa tttggtgctt acagatggta aagctggaat ggcaggttcc
    25801 atagaaaaag caaatgaact tttaaaagaa aatccaaact ataaaagctt aaaacaattt
    25861 gaaaataaag ataatccaaa tgcacattat aaaacaacag cagttgaaat tatgaatgat
    25921 gtcaaagatt tagatatatt cgtatgtgga gttggaacag gtggtacttt agtaggaact
    25981 gcaagatatt taaaagaaca aaatccaaat ataagagtaa ttgcacttga accaaaaaaa
    26041 tctccagcta tatcagaaaa caagactgga cctcataaaa tacaaggaat aggtgctaac
    26101 tttgttcctg aaaattatga ttctagtgta gttgatgaag taatattagt agatgatgaa
    26161 gatgcatttg atacagttaa attacttgca gcaaaagagg gaattttagt agggatatct
    26221 tctggagcaa atatatttgg tgctatagag atggcaaaaa agtatccaga taaaaagata
    26281 gttacagtag ctccagatgg agttgataag tatatgtcta tgggaatatt ttaataattt
    26341 tatttgaggg agtgaatact ttgtttaaga aaataaataa agatattgaa tatataatga
    26401 agaatgatcc agcagctagg tctaagatag aagttttttt actttatcca tcagttcatg
    26461 caatgataat gcataggatg gctcatgctc tttataagaa gaagaagctt tttacagcaa
    26521 gattaatatc ccagatatcc agatttatga ctggcataga aatacatcct ggtgctaaaa
    26581 tgggggaagg tatattgata gaccatggaa tgggtgttgt aattggagag acagcggaag
    26641 taggtaatag ggtgacaata tatcaaggag ctactttagg agctacagga aaagatactg
    26701 gaaagagaca tccaactgtt ggtgatgatg tattaattgg tgctggaaca aaaatactag
    26761 gacctttaaa tataggttca aattcaaaaa ttggtgcaaa ttctgttgtc gttaaggatg
    26821 tacctaatgg tgcaacagtt gttggtatac cagcaaaaat agtaaaaata agaaacctag
    26881 aacctgtaaa aaagaataaa aaagaagtgt cctatgagta tgatgaatta gataatgtat
    26941 attatatata ggatttatat aactataaaa taaactttat aaaaattatt ttaaaaggta
    27001 tttttttcaa atttgaggac ataataaaaa agaactgaat gttacagttt tggttcatca
    27061 atctttgata aataagattt taaatttgta gcgtctagga ggattttatt atgaaagcaa
    27121 atgttaatca agatacttgt ataggttgtg gattatgtcc atcaatatgt ccagaagtat
    27181 ttgatatgaa ggatgatggg aaatcacatg ttatagttga tgaagtacca agtgatgctg
    27241 aagaatcagc agcagaagct agagaaagct gtccagttga tgcaatagat gtgcattaat
    27301 aaaataaatg cctataaaaa ttgaattttc aatttttata ggcatttgtc ttttattcag
    27361 ttagtatttc aacacctgtt tctgtaacta aaaccataca ttcccattga gcagataatt
    27421 taccatcttt tgtataaata gtccaattat tttcttcatc aacaaaaatt tcgtgagttc
    27481 cttcatttac cataggttct attgtgaata tcatcccagg aaccaatagc atctcagtcc
    27541 cttttttaga tacataactt acaaaagggt cttcatgaaa gcttaatcca actccatgac
    27601 cacctatttc tctaacaaca gagtatccat ttttctttgc ataagagttg atagcatcac
    27661 ctatatctcc taaaaatccc catggtttgg caacttcaag acctttttgg acacattcct
    27721 ttgtaacttg aactaatttt tttgcatttt cattaacatt acctatcata aacattcttg
    27781 aagcatctga atagtatcca ttaaaaatag tagaaacatc tacatttata atatctccat
    27841 cttttaagat aatgtcctta cttggaatac catgacaaac ttcattattt actgatgtac
    27901 atacactttt aggaaatcca ttatagtcta gtggagcagg gatagctcca ttttgtagtg
    27961 taaagtcatg aaccaactta tttatatctt ctgtagacat tccaatttcg attttatctg
    28021 aaatataatc caaaattttt gtgtttatta cagcactttt ttttatttct tcaatttgac
    28081 tttcattttt tataatatca tgagttggta caatatgacc taacttagca aatgaatcta
    28141 ttttattatc catatccata tgacattttt tatatttttt agtacttcca caccaacagc
    28201 tatcatttct atttaacttc ataacttttc atccttcttt tattatttat ttaaattatg
    28261 ggttacataa actattttaa aacataacca ttataaatat tataacataa atactaagaa
    28321 atcctttata gatagtgatt tattcaaagt ataaattatt atatggttat tgttgatgaa
    28381 aagaggatag ttaaatgtat tattggatat tagcgcactt aatagtttaa ttcaagtttt
    28441 tcaagaaaat catatgttag aggacaattt tcctttaaag tattttttga atcttcatca
    28501 agataataat gcacaaatga ttctgcaaaa aattcttcta taaacttgct tatatatgca
    28561 tgtgcaactt gcttagggtc ataaagctta tatctttcct gagcaaatac ttccataaat
    28621 tcttcagaat ttgatttttt atttaaaaca tttttatcta tagcatgtgc agtttcatgt
    28681 aattctaaat tttttgatga atgtacattt gcatagctag ggttactata acctatttta
    28741 gccactatag aattatttct atatatacca ggtacatctt tccaagtctt gcctgatctt
    28801 acccaacatg gaggtaattg atattttaaa tcactaaatt ctggttcatc agttagattt
    28861 gaatttatta attttatttt tatatccttt tctttaagtg tctgtaaata tttaatattt
    28921 atttttgcaa gtctgttttt catattttca acttcatctt tatcataatt caagtcatca
    28981 ggaagtataa gcatttgttc aattatatct atactactgt cttttatatt ttcagattct
    29041 tcattaaatt tatattgaag agtattattt tcatagatag ttatatttgt attattttct
    29101 atagaataag agtgaatatc ctctaaatct aaaatattca tttgttgtaa aatatgtttt
    29161 gtttcgccac tccatactac taaagaaagt gaattaaaat taattagtgg attttctttt
    29221 gcttttatat cattgcttat atctacatca aatacatatt caattgagtc ttctatgtta
    29281 agactatctg tattcttttt aatatataaa tacgcttgtt ttaagtctat ataaatctca
    29341 tctaaagatt cgtcataaaa ttctgataaa ttattagtaa agagttcttc acttaaaagt
    29401 tcatttttat cagttatact ttcaattaaa atattaattt cttttatcat atcgtaaata
    29461 cttatttttc tatttgcttt ttcactagat acattgcatg gcgcaagagc aatgcaataa
    29521 aaatcttttt cttttttata aaaggcttta agtgattttg ataaaagtgt aaagtctaca
    29581 cctatctgta agttgacctt aagtgagtca ttttgtaata attcagtatt atttctttta
    29641 gctgtattta aattgttttc cataatcatt gccccctttt tattagggta tcataatttt
    29701 tttatatgtg tcaatttctt tttttgaaaa ttataatttt tttgaaaatt ttatttttta
    29761 taatttgtga attttttatt atagaaataa atatgttatt attatgtcaa aatttagcat
    29821 tagattcatg tagagtatgt tttatacata tattataatt aattctttga agattgcata
    29881 ttcaaaatac acaccatttt ttttaattct ataatataat atttatatat ttaatgttag
    29941 gatgtgattt aaggtgtatt ttacatatat tataagatgt aaagatgatt ctctatacac
    30001 tggatatacc agtaatatag tcagaagaat gaatgaacat aagttaggaa ttaattcaaa
    30061 atacacaaga gcaaagggat ttgaaaaact agaagtttat tttgtgacca acacaaaaag
    30121 caatgctatg aagttagaat attatataaa aaaattaact agaaataaaa aattgtctat
    30181 aataaaaaac ccaagtatac ttataaattt aattgataat aaagaagatt atattattgg
    30241 taaggaaata gaacaattaa cataaatatt tatagtaaca ttataatggg tgcatatagt
    30301 gtaaaaactt gacaataaat actattgtaa tataatgaag ttataaatat agtaaattaa
    30361 aatagctagg ggagccaaga gctgagaggt ataccgaccc ttacacctga tctggataat
    30421 accagcgtag gaaagcttag taaaaattca agtatgtatt atttgtgata tatgctattt
    30481 ctaagctata cctattgtag gtatagcttt tttaattaag ttatttaaat taaactttag
    30541 gaggcaacaa tgagtaatta taaaatacca acacttacaa tagctggatc tgattcatct
    30601 ggaggagctg ggattcaggc tgatttgaaa acgtttagtg caattggtac ttatggtatg
    30661 agtgtaataa cagctataac agcacagaat actaaaggag tttttgctgt agaggattta
    30721 aataagaaaa taataaaaaa acaaatagag gcagtctttg aagatatacc tccaagagct
    30781 gtaaaaatag gaatggtttc aagtcctgag attatacttg aaatagttga gaatctaaag
    30841 aaatacaatc caaaatattt ggttgtggac ccagtaatga tatctaaaag tggatattat
    30901 ctattaaagc cagaggcaaa agaaaactta attaagtatt tgataccact tgcatacata
    30961 attacaccaa atataccaga ggcagaagaa ataacaggaa taaaaataca taatgtagat
    31021 gatatgaaga gagtaggaga agaaatacta caacttggac ctaaatttgt acttatgaaa
    31081 ggaggtcatt tagatggaga agcagtggat attttagttg gtaaaaatat atttaaagtt
    31141 tataaaagtg aaagaattga taaaaaaaat acacatggaa cagggtgcac attatcttct
    31201 gcaataacat cttatctagc attaggatat gaaataacag aagcagtaaa tctaagtaaa
    31261 atctatataa cagaagcaat aaaaagaagt tttgatatag gtcatggagt aggaccagta
    31321 catcattttt ataaatttga gtaataaaaa aatattggag gtagaatatg tataatttaa
    31381 ttaaagatgt aaaaaaatta aatccattgg taattcatta tactaataat gtaactataa
    31441 atgattgtgc taatgtaact ttagctgtag gagcaagtcc tcttatgagt ttttcatatg
    31501 aggaagtaga agaaatggta agtgtagcaa actctgtagt aataaatata ggaactatga
    31561 actcaaatat gttggattta ttcctattag ctggaaaagc agcaaataag tacaataaac
    31621 ctgttgtttt ggacccagtt ggagtatttg taagcaaagc gagagcagaa cttacaagta
    31681 gacttttaaa tgaagtcaaa tttagtgttg ttaaagggaa tgtatcagaa ataaagttta
    31741 taggtggctt taatgtaagg ggtaaaggtg tagattcatt tgatgaagaa gaagattcta
    31801 ctgagataat aagaaagatt gctgaaaagc ttgaatgtgt agttgtagca actggaaaaa
    31861 tagatataat aactaatggt aaaggtacat ataaaataaa caatggaact gataaattaa
    31921 aaggaattac aggaactgga tgtatgactg ctagcttgat tgcaagtttt atggctgtta
    31981 ctgagaatat attagaagca gcaacaatgg gtgttcttac aatgtcttta agtggggaat
    32041 tagctaattt aaataatcca ccaataggta catttaaaga aaatcttatg aatgctattt
    32101 atcaaatgga tatagatact ttaagtaaaa attctaatat tgaattttta aattaataaa
    32161 gagtaaggga gacttaaaat gattgataaa gaatctttaa aaaaatgttt aaagttgtat
    32221 ttagtaactg actctgaaat gcttaaaggt agagactttt ataaatgtct tgaagatgca
    32281 atatcttctg gaataacaac tgtacaactt agagaaaaaa atgcttctgg aagagagttt
    32341 ttaagaaaag ctatgaaact tagagaaata actaaaagat atggtgttaa gtttataata
    32401 aatgatagag tggatatagc acttatttgt gatgcagatg gagtacatgt aggacagagt
    32461 gacattgatg ttagagaggt tagaaagctt ataggaaaca ataaaattct aggagtatca
    32521 gcaagaactt tagaagaagc tatttgtgca aaaaatgatg gcgcagacta tttaggtgta
    32581 gggtctatat tcacaacatc tactaaatta gatgcaaaat cagcttcgtt tgaaactgtt
    32641 aaagaaatta aagaaaaagt agacatgcca tttgtactaa ttggaggtat aaatttagac
    32701 aatatagata aacttaaatg tttagaaagt gacgggtatg ctataatatc agctatatta
    32761 aaagctgaag atatctctaa agaagtggaa aagtggacat taaaaatata aaattggctc
    32821 tggtagtgaa aaaaatggaa aaatttgata taatctaaga gtaaaattta agcaattata
    32881 aatgataaac ttttctacat atacttttta atcaagatat atgtagattt ggttaaaaag
    32941 gagctataaa tgattaggat attgttagtg tgtgttggag gcatgtcttc taccctattg
    33001 gtaaataaaa tggagaagga tgcaaaaaaa agaaatatag actgtaaaat atgggcagtt
    33061 ggagaaggag atataaaatc ggagcttgat aactttgata ttttacttat tgggccacaa
    33121 cttagattta tgcttgatga cgtaaagtct atagttggag atagagctcc agtatctata
    33181 atagatatgg taaactatgg tacttgtaat ggtcatgctg tgttaaattc ggttttagaa
    33241 atattaaaat aaataataaa agtggattct atataataga gtccactttt attatttgtt
    33301 attaataggc ttaaaactta gcttaccatt tcttttttta atctccacat agcaagtgaa
    33361 aaacttgaac agcacaatat taaaaatagt aggataacag gcattatatc tccaataaga
    33421 atagtctcac caaatattaa gctacgtaaa gcgttaataa catgggtaaa tggattgagc
    33481 atcactgcta cttttaatcc tccagataaa ctttctatag ggaaaagagc tgaacttaaa
    33541 aagaaaatag gaagtacaat tgcagtcata attgtttcat aaactatttc atttggcaat
    33601 aataaactta tagaataagc tagacttgat aaaaagaagg ctgtcataaa tatcagtact
    33661 atcataagta ggattccacc aatgccagat tctatcctta ctgagaagaa tatacttact
    33721 acacaaaata gttacttcta tgaaagaaac taagatagct tctaataatt gaccaaggac
    33781 gattgaatac ctactgatag gtgcaattaa gactctatag aaacttccac tgttcttcat
    33841 tataaagttt ataataccac cactactaca agaagaaaac acaaccagta caatgatacc
    33901 tggtagtata aaagcagtat aattattgat atttatatta ttcatagttt ggtttgcaat
    33961 agaactataa agcacaagcc aaagaaaagg ttgtaatata gtaactacaa atgagattgg
    34021 atttttaaaa cgccacttca tactacgcca taagatattt aacattccca ttcctccttt
    34081 aagctttttt tagaacttgt aagtgctaaa aatacatctt cgagacttgg ctctacaatt
    34141 tctattgcat caaattctat atcatgttct aaaagccatt tatttattaa agtaaaagca
    34201 gttctactat cgtttacact tataaaaatt gaattttctc gtacgctcat aaactttact
    34261 aaaccagtac ttttaattga atctttatat ttttttatat cttcagtatt atggaaagtt
    34321 atacgtagta tattttgtct aatgtaacta cgtaaacttg atggtgttcc ttgtgctaaa
    34381 tcctttccat tcttcataat acaaatatta tcactcaatt gttcagcttc ttcaagataa
    34441 tgagtagtta gaaatatagt tgtgccgtac tcatctctaa tttttaataa catatcccac
    34501 atagattttc ttgaatcgac atccatacca acagttggtt catctaaaaa taggatttta
    34561 ggagatgata ccatattcat agctatgtcc aatctacgtt ttacaccccc tgaatatgat
    34621 gaagtaggat attttaagta actagataag tcaaacttat caattaaaga gtctattctt
    34681 tgttttgcaa tctgtggttc tactttatac attttacttt gaaataccat attttccata
    34741 agggataaat gttcatcaat agagatttgc tgtgctacac aagcaatttg agtacgtatc
    34801 caagaagaat tatctactaa atcttttcca aacattttta catttccaga tgtaggtttg
    34861 taaaaggtag tcaaaatatt tataagagaa gatttaccag caccattagg accaagtaaa
    34921 gaaaaaattt ctccagcatt tacattaaag cttagaccat ttaaagcttt cacaccattt
    34981 ttatattctt ttactaaatt gtctatttca atcgctaaca tatattattc ctcctcttta
    35041 agtggaaata ttatctctgt aatatagtta tctgggtttc cttttatcaa catacctggt
    35101 cctttaataa atacttctct ccaaggagtt tgtattttta tattattttc atgaatatat
    35161 ttaaatatca tttcatacgt ttttggcaaa ttactataag agcctgtgtg agtaaaaaat
    35221 aaagcttttg ttttaggaaa ttcttttgta gttataccca tacgatctgg agtttcagca
    35281 gttggaacac ataattccat ttctgcaaca ccagtttttt ggtcaacatc atagtagcaa
    35341 aagaatggtg caccgttaga ctttcctttt atagctttaa atacatttgg aaaatacttt
    35401 gttgcctcag tcataggtcc attataacgc atggtagcca ctgtgactga ttcgatattt
    35461 ttgatttcaa ttttgcaatt catttgttta cctcctaatg tgtgttcata ttcttactag
    35521 atttttaagc attaatacat ataagatttt gattagttga aaacatataa tcttatctcg
    35581 tttgcttgtt ttcaagtcta acataacata aaatatatat cctctcattt gatgctccat
    35641 tttgtaaagt tattatttaa gctttacttt agatttttta gcaaattaaa ataaagcaat
    35701 ttataaaata agtatttaca aattaagtgt attaatcgta taatacactt aataggatac
    35761 tagataaagt ctgaaataaa ggcgtatttt tttaaagtaa gtgtactatc gtattaatac
    35821 actagaatag gaggaatcat gaattttgaa ttagataata caactcccat ttatttgcaa
    35881 atagtaaagt atataaagag acaaatagta acaggagagt tgaaaccagg agagacaata
    35941 ccatcaagaa gagaaatggc acttaatcta aaagtaaact taaatacagt tcaaagagca
    36001 tataaggaga tgggtgatat gaatataata aatactttta aaaattatca aagcagtgta
    36061 acagttgatg aaaatatatt aaaaaattta aaattagagc ttataaatga atcactatct
    36121 gtttttatag aagatatgaa agcaataaat gttagcaaag aagaagtttt aaaaataata
    36181 gaagataagt attagttttt taacaaatag aaaacttagt ttagaaagtt tatttataag
    36241 catattggat gtggaggata gtaaatatga tagaaataaa gaatgtcagc aaaacatata
    36301 aaaggatgca agggcttaaa ataagaaaga tagaagcttt aaaaaatgta agttttaata
    36361 tagaaaaggg aaaaataact gcattacttg gtataaatgg tgttggtaaa tcaaccatgt
    36421 taaaagcaat agcaggactt ataaacattg atagtggaga aattcgtata gatggagaaa
    36481 aaataaatga aaaagtatac aataaattgg cttttgttcc agatgtacaa tctcacttta
    36541 gcaatacaac aataaaagaa acatttgaat ttatggaaat tttttattca aagtggaata
    36601 aagaaaaatc aaaagaaatg atggacatat ttaaattgga tgaagatgaa ataattgata
    36661 acttgtcaaa aggaaacatt gcaagagtaa aattaatact tggattttgc caagacccag
    36721 agtacatact tcttgatgaa ccatttacag gaattgattt gtttaagagg gaagagttta
    36781 taggagtaat agcgcaatat atggaggaaa atcaagccat tatcataact actcatgaga
    36841 tagtagagat agaaagttta gttgatgagg ttgtaattct tgatgaaggg cagattataa
    36901 cttcatttaa tgcagaagaa ttaagagaaa gagaaggtaa atctatatta gataagatga
    36961 gggaggtata taagaatgaa taaacttcta tatctaataa atgtagattt gagaagaagc
    37021 aataagtttt atatagctta tatatcattt ttttcattaa ttacacttag tttaaattta
    37081 ttcgaaatta ataaatttaa aaattctaaa tttattatga acttagcaat agaagatttt
    37141 ggaggtatat tttatggact agggatactt agtaatcttg gttttatcca aagtatattg
    37201 ttctttggga tattgggact ttttatatac tatattttta tgtggaaaag agagttcttt
    37261 tcaaataatc aaagtattta tacacttatg atgttacctc aaaataagtt taagatttgt
    37321 atatctaaat caatagcctt gataacaatg atatatggtt ttttgataag tcagacagca
    37381 acattattta tatgtaaata tatatttaat ttcattttta gaaatatgcc tataatcaat
    37441 atgaactttg ccaaagattt atattattta aacttgaaga gtataccaat tgattttatc
    37501 tcttttatgg ctatttatgt atttttacta cttatagcta tttctattgt aaattgctgt
    37561 atattattta tcttttccta tataaataaa ttttatgtaa ttttaatcac tttgctgatt
    37621 atagcaggct tatatttttc aacaatatat ataaatataa tcactttatt tagaaaatat
    37681 tataatctta taggatttca atttaacaat ttagtagcag ggttattaag ttttttaatt
    37741 ataatgttta ttctaaatgg aatatcttat ttgctaatta aaaagaaagt atctctttaa
    37801 ggaggggtgg tatgaaatta aaatctaaaa tatctatatt tattatattt ttgactttag
    37861 caatatattt tagtataggt ttattaaata atgaagataa tttaaagata aaaactataa
    37921 aaggtgactc taatgaatta gaccatataa acataaattt tggatatgct ttgaatggat
    37981 atataaattc atattataca ataaatggaa gtaacaaaac caaaaagact gaattaataa
    38041 aactacaaaa ggatagtaag aacacaaata atttaattga tgtatctagt ggttcagatt
    38101 taattgctca tgttggattt gaaatgaatg aggagtcaaa agatataaac tttgtgatag
    38161 atgagaaaaa agaagattta gaaaaaggtg tatttccatc agaaggtagt gataaagaaa
    38221 ttgaatatga aaaattagat atgaataatt ttacaaggac aagatataaa gtagataaaa
    38281 gtacagatga ttattatgca aagatactag catcaaaaag atataaaaaa tacttatatg
    38341 tatctgtact ttatgctaca agtgattcat cagaaaataa caaagaatat tcaagtacta
    38401 tagatataat aaaaattaat ccagaaaata aaaaaatcaa gactataaaa agtataaatt
    38461 taagtaatta tttttccgaa aaataccaaa acatagagaa cataggtgta agttatgcta
    38521 gcaaatatga ggataagatg tatattttag taaatgtact aaatacaaac ataggtgata
    38581 atagtaattc taaatttcat ttacttgagt ataatcttga agaaaataag gaaagcataa
    38641 tggaattaaa aactgatagc aaaatggctt tagcaaaagc aaagttagaa gctaataaat
    38701 tatatatggt aatgtcgaat gattatgata atagttcaat tacagagtta aatttactta
    38761 cctataatat attagataaa agcttagatg aagataaata tgttttagat aatagagaaa
    38821 aaagaggaat tagaagtatt gaaattgatt caaataaaat cgttgcatat acatcaaggc
    38881 taaactctat attatcttgg gatgataaat ttatgctata tataataaat aaaaattcta
    38941 aggaaattgt atatgaagga gaattacaat ttaacagttc aaatagtatg ttagaatttg
    39001 aaataaagta ggtgagttga tgaaagattt aataagatta ttcgaatttg aattaaaaag
    39061 aaatataaag aattatatat ttataataat ttgttgttgt tcttttgtaa ttttaaacat
    39121 agtaaaaaat ttaaatgact ataattacat aatagaaaat gcagttaaaa gtgaacagtt
    39181 aacaaaaatc ggaaatacga tagaaacggt aaatgttaca ggtttctcaa gctttagaaa
    39241 tataatgggt tctacagaat cgtggtttat gtttggtata attgcatgta tttgctatgc
    39301 tttttttatt tggtatagag actttaatgg tagaagtaaa tctatatata cacttataat
    39361 gctacctaaa aatagaatca atat
//
LOCUS       DAEMHZ010000019        38965 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000019 DAEMHZ010000000
VERSION     DAEMHZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.019"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            174..797
                     /locus_tag="KPK72_000897"
     CDS             174..797
                     /locus_tag="KPK72_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488139.1"
                     /translation="MIEKLNESASLSQLITAFENSTNEIKTIKENIVNAVGNPITSND
                     KLSVIPNKLNDILSEKDKTITQLNAKYKVATGTVTSFADDTKIAYPYRTDRAVKPGTW
                     VKISNLDFKPNIFFADFDYYDIEYKNNYKLFLFACRGVTTQGGVDFSSVTAFIRKNSD
                     EHFHAKGWLYGNSEGDVYFNNAGVQIPAYSFNSTQRHIYKWYAIKFI"
     gene            812..1108
                     /locus_tag="KPK72_000898"
     CDS             812..1108
                     /locus_tag="KPK72_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488140.1"
                     /translation="MNVPNRVIYDQTGRIIFETGESCGDVLPHDKITELHYIDVEYGS
                     IDYTRNKIVGINIETKEPVLEEIPAYISKAEKEKQELENQLLLMTNKELGGGIL"
     gene            1108..1272
                     /locus_tag="KPK72_000899"
     CDS             1108..1272
                     /locus_tag="KPK72_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488141.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQEIK"
     gene            1470..2081
                     /locus_tag="KPK72_000900"
     CDS             1470..2081
                     /locus_tag="KPK72_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488142.1"
                     /translation="MIEKLNENASLSDLITAFENSTKELKASKDNFTNLLGNPFLEST
                     KFSEFEGKMQNLMSTFKDNLKSKGVSSGNTESLLSLINKVASIYIPTPIYTASGECNI
                     SYSGECKSYTLSGSSSVYFSKINLNFYPRFILIYNTGGASKYFTTSYFCSSVPFIAST
                     VESYTTYLLDRSDKQVQIQKGECVLPISSCRYASPYFYFYAIG"
     gene            2093..2398
                     /locus_tag="KPK72_000901"
     CDS             2093..2398
                     /locus_tag="KPK72_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488143.1"
                     /translation="MNDYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYVDYDKYKIIGINIETKQPILEEIPIYMSEEEKRIQELENQLLIAENEKVGGLL"
     gene            2540..2803
                     /locus_tag="KPK72_000902"
     CDS             2540..2803
                     /locus_tag="KPK72_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488144.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            2804..3601
                     /locus_tag="KPK72_000903"
     CDS             2804..3601
                     /locus_tag="KPK72_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2488145.1"
                     /translation="MKIALTAGHTLTGKGTGATGYINEGTENRILMELIAKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     ADRVNTKLATVFKNRGAKLDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIENSEDKKMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYV
                     PGKTENLYVVGGRACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIKK"
     gene            complement(3628..3837)
                     /locus_tag="KPK72_000904"
     CDS             complement(3628..3837)
                     /locus_tag="KPK72_000904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488146.1"
                     /translation="MKDVFEELISIKDACELYKKGESTLRLNIRNGKFVEGVDCKKFG
                     TTWVFKKTALEREYNKKDKKIESGN"
     gene            3986..4285
                     /locus_tag="KPK72_000905"
     CDS             3986..4285
                     /locus_tag="KPK72_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488147.1"
                     /translation="MKKITKKEIKKYVREAVNNNFNWEINKCGFYIKNNEIEFFISYK
                     GQGIDENVYNDTYEEIIYIEDIIEEYRRKEYNLEDVDSIVYDNVNDMINCYNEEK"
     gene            4374..5009
                     /locus_tag="KPK72_000906"
     CDS             4374..5009
                     /locus_tag="KPK72_000906"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488148.1"
                     /translation="MKIGDKFENLTILDIEQKNSRKYCLCKCKNCGNEKWIRADSLKR
                     IKGCGCLQKETQFKQNDLTNKKFGRLTALKNTNKKAKSGHYIWICKCSCGNEIKTTEN
                     NLTTGRTKSCGCLKKESNIKNAKVALKVHKEKNIIDDTNLSIIKKTEAYPNSKTKIRG
                     VSWNKEKRKYCAQIEFKKTHYNLGYYDNIREAEEAYKKAKEKFLKEINGKV"
     gene            5086..5556
                     /locus_tag="KPK72_000907"
     CDS             5086..5556
                     /locus_tag="KPK72_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488149.1"
                     /translation="MNKQKARRFLRVIDMNIDKIEEEAIKAFKESCLIKETNNIKIYI
                     DIQGKVEAIAVQTWAKLLDDNKEINIFTLNQTPTHLNDMLGEICYVNDYEEFENWCEN
                     EWENLDWDSYKKFNKENFEEIAERNIDDSTSVFLEELQKGIESCKQELQNIVEN"
     gene            complement(5858..6139)
                     /locus_tag="KPK72_000908"
     CDS             complement(5858..6139)
                     /locus_tag="KPK72_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488150.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVVNFLRYAGVDKDMKIKL
                     FQTIIEAVARKKITLPNDSKFTEGILQSSEFEMTENVGFILEGLSYRNN"
     gene            complement(6283..6618)
                     /locus_tag="KPK72_000909"
     CDS             complement(6283..6618)
                     /locus_tag="KPK72_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488151.1"
                     /translation="MPKFDGELLSNKEKPVFKRNGTIIQNDSTTTDNKTLNIYNLVNK
                     KSKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNER
                     NRRNKTKKK"
     gene            complement(6619..7428)
                     /locus_tag="KPK72_000910"
     CDS             complement(6619..7428)
                     /locus_tag="KPK72_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG2488152.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNKNYTNIDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIILIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTVLVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFANMLEELKVKGVL"
     gene            complement(7716..7898)
                     /locus_tag="KPK72_000911"
     CDS             complement(7716..7898)
                     /locus_tag="KPK72_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG2488153.1"
                     /translation="MKKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            complement(8720..8872)
                     /locus_tag="KPK72_000912"
     CDS             complement(8720..8872)
                     /locus_tag="KPK72_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488154.1"
                     /translation="MDNFLLNILADVIASLIFYIISKLFRQVKNHSAQKSGWEFDLKI
                     KFKKSR"
     gene            9335..10042
                     /locus_tag="KPK72_000913"
     CDS             9335..10042
                     /locus_tag="KPK72_000913"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488155.1"
                     /translation="MSLEEAKELEKNYKLPMPTFCLKEKGYFAREYAALMLLSNGGMK
                     IMHTEKNRYLYQNKFDIDALAKQLSISRATLYRNIKKLEELKCGILKIENTKNGTVYR
                     LKYGIETGYNDEVHKFVTIHHKILEELVINFKTPSVKLYCLLCYLTNEIDFKVIDEEF
                     LCNNIGLCGKSESNKNKVRSMIRVLMKCKYIDVKKENRTFWNEETKNKINKTLKLYRL
                     CSYEEWKNLNDKTDFKD"
     gene            10588..11262
                     /locus_tag="KPK72_000914"
     CDS             10588..11262
                     /locus_tag="KPK72_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488156.1"
                     /translation="MMLKYITKHKNTEDLRMKKVLLLITVCLLSVGLVACSSTNNKDA
                     GNTTKKEKKANENEVDGIKITIKNVTKEDIKGDINEDGSFNENGEYFSDGIEKKKAAD
                     YVYEVVNVEVENKTDKAVKLFQTGWNAVGTDGYEFKDIKATDKLDNQQVPANYKFDAQ
                     VKILVEKNMNVKEIVLKYNLKDYSRLFEAMEYANQGASKSDVEKKFPELYKDNWIELG
                     EIKVEQ"
     gene            complement(11286..11588)
                     /locus_tag="KPK72_000915"
     CDS             complement(11286..11588)
                     /locus_tag="KPK72_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488157.1"
                     /translation="MDTLLKYHRKKKKLTQRRLSELTGISQSYISKMENEKFKHSPTL
                     TQIILLADVLEIDQLVLSDFFLEKEREHLKNTKKYNKKAVKTLSRDFLKEYKIRTR"
     gene            11828..11983
                     /locus_tag="KPK72_000916"
     CDS             11828..11983
                     /locus_tag="KPK72_000916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488158.1"
                     /translation="MKKSQIKTKNRYVENKIETAICVNELKYISVDDYVFLKECIEKI
                     YIKNKRT"
     gene            complement(12017..12385)
                     /locus_tag="KPK72_000917"
     CDS             complement(12017..12385)
                     /locus_tag="KPK72_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488159.1"
                     /translation="MDEISKETINKEIGKRLNLLRTNEKLSQKAFGEKIFLSQDQISL
                     LEKGKRVLTERSINDICREFDVNEEWLRKGIGDIYKDCLSELEIDEDVKEITNKLYEL
                     EYEDRNAILQMIELLHKKNN"
     gene            12556..12732
                     /locus_tag="KPK72_000918"
     CDS             12556..12732
                     /locus_tag="KPK72_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488160.1"
                     /translation="MVYSSKEKIETALMIEFLKKNDPRNWESIETLISSTYTLSSFKK
                     GNKISQKRGDKIEL"
     gene            12713..12928
                     /locus_tag="KPK72_000919"
     CDS             12713..12928
                     /locus_tag="KPK72_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488161.1"
                     /translation="MIKLNFNKYQKGEIVQLYETKFSCNRNCIVTYADEKEIVLMYYD
                     KEIKELIYKTLTKEDLIFNDYELKLLS"
     gene            12960..13832
                     /locus_tag="KPK72_000920"
     CDS             12960..13832
                     /locus_tag="KPK72_000920"
                     /inference="COORDINATES: protein motif:HMM:NF014080.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2488162.1"
                     /translation="MELLKDSRKRDWFWVENDLIDREDLTIYEKMIYMTLVRHLNENS
                     FCFPSHKTISKKSGCSESQVKKVIKSLENKELIRKENRTKLNSKEKDSNIYFVLTAKI
                     SRAYGDLPVGHSETHPRACGDLPVGHVVTSNKTNINKTNLTNNIYSSAEQKEEKTKDK
                     EIYKRIVDYLNKTADKSFKSTTKKTISLIDARLKEGFTEEEFYKVIDNKVLNWLDNDK
                     MNAYLRPETLFGNKFESYLNETPKKSLKGNESVKGKTKTNNNKFANFNQTFDKYSEDE
                     LDAIIKKSQSAKFK"
     gene            13857..14459
                     /locus_tag="KPK72_000921"
     CDS             13857..14459
                     /locus_tag="KPK72_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488163.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKIPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            14505..14786
                     /locus_tag="KPK72_000922"
     CDS             14505..14786
                     /locus_tag="KPK72_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488164.1"
                     /translation="MIKNNVLSYEEKIKVLKEHGYNDSTKEYILKEFGKEHIFYESFL
                     RAIERDDSKKSVLIKKFEKGQHQFSEGFIKTHTISELLKEEERIRKILG"
     gene            complement(14829..15173)
                     /locus_tag="KPK72_000923"
     CDS             complement(14829..15173)
                     /locus_tag="KPK72_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HBG2488165.1"
                     /translation="MVTNQDCIRDILLFIESNTTPISPVLQFDTILDNLKYDESTIIH
                     HINLIYQNSLVDDVNYGDDTVLEVTQLSLKGYQYLDEIRDSTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            15262..15438
                     /locus_tag="KPK72_000924"
     CDS             15262..15438
                     /locus_tag="KPK72_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488166.1"
                     /translation="MQKRITEMSILEKVDLIGQKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKYFEYR"
     gene            15464..15790
                     /locus_tag="KPK72_000925"
     CDS             15464..15790
                     /locus_tag="KPK72_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488167.1"
                     /translation="MEELKEEIKQKEENLKEREKYIKELKERLECFARDALESEECII
                     DIENILIEQTVELEAYNLFIDCILNKDIRKAIAIYNRTKSIRIKHKNINSISKKIEKI
                     KQIISL"
     gene            15810..15983
                     /locus_tag="KPK72_000926"
     CDS             15810..15983
                     /locus_tag="KPK72_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488168.1"
                     /translation="MDKFVTCARCERTINVEENNHVKYEEETLNLSFTLYFCLGCVDK
                     LIEQSKMLEGENE"
     gene            15976..16191
                     /locus_tag="KPK72_000927"
     CDS             15976..16191
                     /locus_tag="KPK72_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488169.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYTLEYRSKFYEWIKLLE
                     RKGSLQNYKIVEVLNAERNGEEASFWN"
     gene            16250..16423
                     /locus_tag="KPK72_000928"
     CDS             16250..16423
                     /locus_tag="KPK72_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG2488170.1"
                     /translation="MKMINVFKLSSMYCICPKCGSDKLGEGEGKLIVDEHTYYRECKC
                     GFNVLVDEREDEI"
     gene            16438..16845
                     /gene="ssb"
                     /locus_tag="KPK72_000929"
     CDS             16438..16845
                     /gene="ssb"
                     /locus_tag="KPK72_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2488171.1"
                     /translation="MNSVILIGRLTKDPELKYIPGSGTAVSTFTIAVDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLGKGRLVAVEGSIKVNSYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTEKEYMFEPKGLDQQGFQAIDDPDIPF"
     gene            16862..17011
                     /locus_tag="KPK72_000930"
     CDS             16862..17011
                     /locus_tag="KPK72_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488172.1"
                     /translation="MSNINKKTAKICGWCGTTVYSYVDGKIAYCSKKCKKKREKNLEI
                     EGAKK"
     gene            17008..17289
                     /locus_tag="KPK72_000931"
     CDS             17008..17289
                     /locus_tag="KPK72_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBG2488173.1"
                     /translation="MKYIASFSGGKDSAAMLLLILEKKLPLDEIVFIDTGLEFEEIYN
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNKQGKRKGQIWGFPYLLGAW"
     gene            17410..17700
                     /locus_tag="KPK72_000932"
     CDS             17410..17700
                     /locus_tag="KPK72_000932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488174.1"
                     /translation="MKKTKIEEFITKVKESMQSVAKESIKDLENILKEIETGETEVKI
                     LFIDKEKKVEEIVEFTHDEYVKMIIEKMIFDSLKIIKEQHEKILKEFEEREV"
     gene            17703..17963
                     /locus_tag="KPK72_000933"
     CDS             17703..17963
                     /locus_tag="KPK72_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488175.1"
                     /translation="MNFKMIANIPKWTVKEQFEKVLEEVLELKEAIELKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKEIREGLKLHNKKLRKRKWKLEKIN"
     gene            18434..18895
                     /locus_tag="KPK72_000934"
     CDS             18434..18895
                     /locus_tag="KPK72_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488176.1"
                     /translation="MKIKKEYFDKVENALKMLGDKYRKLRVLKKEIAILKHKENYREI
                     NYEELGFKVQKSVKGIDDMVVTIEDAIYNKETEIEIIERKLEFYNIYLKELSEIEQKI
                     IELVYFYSWNGKMPITKMAMELNYDRTSLYNKKAIAINKIALMIYGDEALE"
     gene            19075..19623
                     /locus_tag="KPK72_000935"
     CDS             19075..19623
                     /locus_tag="KPK72_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2488177.1"
                     /translation="MNFVEPIRNLEKLESICAYLEETNMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITLKESKTGKTKPIEVNPILRRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            19796..20068
                     /locus_tag="KPK72_000936"
     CDS             19796..20068
                     /locus_tag="KPK72_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488178.1"
                     /translation="MNKYGKFKCNNCGKKVNVNNRDLKTKVIDNTTVTYFMCRRCFEK
                     FVVSCEDDYIKHKREEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            20058..20390
                     /locus_tag="KPK72_000937"
     CDS             20058..20390
                     /locus_tag="KPK72_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG2488179.1"
                     /translation="MSYKHMTLDRLKELILIDRLDIFYRSTDWRKLRKKALERDNNEC
                     QICKEKKKVSKAECVHHIKEVKEYPGEALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSEERW"
     gene            20562..21068
                     /locus_tag="KPK72_000938"
     CDS             20562..21068
                     /locus_tag="KPK72_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HBG2488180.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGYKKNDSVSELIKYNAQMLSLLDKLGIKATPKV
                     LGDDDIEL"
     gene            21052..22680
                     /locus_tag="KPK72_000939"
     CDS             21052..22680
                     /locus_tag="KPK72_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBG2488181.1"
                     /translation="MILNYKYNKYIDEYFNLVDKGEIVLCKEMELAVKKIKKDLKKPN
                     IYIDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKN
                     GFISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTK
                     EVITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPR
                     VFYITTDGNVRGGVLDDFKEESKQILNEEIKNSKMLPLIFKLDSIEEMENIDLLEKAN
                     PSIKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAQNLATVVAEWDDIVATNEE
                     LPDLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIEL
                     AKQKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFANKGLPIE
                     EVRNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPI
                     KRKTDGFFAFLHALSKDGELMQESTIKFYDCYTY"
     gene            22744..23889
                     /locus_tag="KPK72_000940"
     CDS             22744..23889
                     /locus_tag="KPK72_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2488182.1"
                     /translation="MSGADGEYFKKLEATIFYKEFALRSCISIIANALVLSEFQTFIN
                     SKSVKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQENEQLFVAESFNVDK
                     YVFYEDVYKNITIRNYKLDRSFRESDVLYFKLNVESIKTVIDYLYNDYKDLLSSAMKG
                     YKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDNAVLPLSNGLKYTENT
                     KASSIKDSRDIRAIIDDVMDFVCAAFHIPAGLIKGNVSGVEGITDNFLNFSVNPIARL
                     ITTEITRKMYGRDKFFEKSYVKVDTQKIANMGLEKISKASDLLFRIGVNSINDNLEML
                     GREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKSGIAGNKGQ"
     gene            23849..24478
                     /locus_tag="KPK72_000941"
     CDS             23849..24478
                     /locus_tag="KPK72_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBG2488183.1"
                     /translation="MEKRAVSLEIRDSNLESRKISGYAAIFNDDYTKLQDRWGDTFYE
                     KISRGAFLKTLADNTRDKFMLINHDWNKVIGRTNSNLILEEDERGLRFELDIPATTDG
                     NDLLENVRLGLIKGCSFGFNIVNSKTRFDDDWTYYRDITEVELFEITATPIPAYNDTE
                     INCRSDISIKELRESQKEKVKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            24482..25648
                     /locus_tag="KPK72_000942"
     CDS             24482..25648
                     /locus_tag="KPK72_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBG2488184.1"
                     /translation="MAIKNLDEATNVELRTNLMEAIKSNDEEKITTSFLRMAEDIQSN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSQVIEKRGFTDLEITMPRTIFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWVIRKTGTEAAWWGQLTDPIKKELEGGFAKINT
                     VAYKLSSYLPVAKSMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPIGMMKD
                     LKGSVVEGVYPDKTAKPLADFKPNTLGTEIMAPLTNGGKRTVTSVIMLVNPVDYWAKI
                     FGATTMLTSNGAYVYGVLPIPGNIIQTVAVPKNKLIVGVAKDYFMGIGSAGKIEYSDE
                     YKFLEDVRVYLAKQYATGTPKDNFSFLVFDIENLDTTLATEINSNTKTSATRAK"
     gene            25682..25951
                     /locus_tag="KPK72_000943"
     CDS             25682..25951
                     /locus_tag="KPK72_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488185.1"
                     /translation="MVEEVKEYLNITWIEEDAKIEKMVVRGINYLENEIAGIALDFKD
                     NFNRELLFNYCRYVRNNAIEYFEENFSKELFRLQIKSAANEVTKI"
     gene            25948..26598
                     /locus_tag="KPK72_000944"
     CDS             25948..26598
                     /locus_tag="KPK72_000944"
                     /inference="COORDINATES: protein motif:HMM:NF017343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HBG2488186.1"
                     /translation="MKKIKFDNFNDGIIEFGEYKESYDIEGNALDEKEFIVNSKLFYS
                     NEYIREQDRLKFDNTGLKVSIKLKVPYMKLIKTSDVIKLNNEFYSIVKLDISNDKTSI
                     YLYLSELVDVLDKHISIFIKERKNVLEDVEMKFYKKVWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            26615..26941
                     /locus_tag="KPK72_000945"
     CDS             26615..26941
                     /locus_tag="KPK72_000945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488187.1"
                     /translation="MHSKIVTILEKLNIGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVI"
     gene            26942..27385
                     /locus_tag="KPK72_000946"
     CDS             26942..27385
                     /locus_tag="KPK72_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2488188.1"
                     /translation="MSVEITTEGFDAVLSKIESMGKSGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSKIFYGKFLEFGASAHKIPIK
                     KGKKKGRIINHPGVSPKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            27385..27720
                     /locus_tag="KPK72_000947"
     CDS             27385..27720
                     /locus_tag="KPK72_000947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488189.1"
                     /translation="MININDLVIETLKPLKIPVSFQRFDGEEKTYITFFSYLEQNRYA
                     DDEVVGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHTIFRF
                     LFFAEKKED"
     gene            27724..28320
                     /locus_tag="KPK72_000948"
     CDS             27724..28320
                     /locus_tag="KPK72_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2488190.1"
                     /translation="MAIIGLRNYRLVKLLVDTESELEYDTKIMKLTGAKNIKIAPKVD
                     SAENYGDDQLLETASAMGAIEVEFEVASLTLEEKAFILGYKYKDGVLVEDKDFNPPNI
                     ALGFESPKSQGGNRMVWLTKGVCEPLEEEAKTKEDKVEFQSQKIKLKFMPRINDGRHK
                     ITADTDVENSPTAEEFFATAFLKTAAKPTAPAKTNLNK"
     gene            28335..28622
                     /locus_tag="KPK72_000949"
     CDS             28335..28622
                     /locus_tag="KPK72_000949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488191.1"
                     /translation="MEIKLTINEEEKIFKAPPIALRKLDKVFTLTDKIEDGLNSTELF
                     RELLDFTVDIYGEQFSKDELLDGFYPAGDFINKALEDLSKVSGGFEEKVKN"
     gene            28706..28861
                     /locus_tag="KPK72_000950"
     CDS             28706..28861
                     /locus_tag="KPK72_000950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488192.1"
                     /translation="MFLFEKNWSMTDIDNMDIFYYLDILVYMKENKKDNNNPNENDVY
                     IDQISWL"
     gene            28874..29092
                     /locus_tag="KPK72_000951"
     CDS             28874..29092
                     /locus_tag="KPK72_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488193.1"
                     /translation="MCKYCEVKINTEPFSMYDTVVRGELLTNDSDIDMYIELKNGKHF
                     LTGASYDYLGISSKETIKYCPFCGRKLI"
     gene            29161..34362
                     /locus_tag="KPK72_000952"
     CDS             29161..34362
                     /locus_tag="KPK72_000952"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG2488194.1"
                     /translation="MAEDVGELVVRVAMENSSFQQGVNNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSLVGKQMQDVGKKMTMSITA
                     PILAVGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMKELGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALISA
                     VQELGLKIYNVLKPSIAQAVSWFQNLTNKLNEMKPSTVETIVKIAGLAAALGPVLLIG
                     GKIVSMIGGAIGAFGTFSAAMAVVTTGAAAATPAVGALAAILGALSSPAAVAIAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSVSESTKEAVGAFMDLEEKTTISLNQLMWSGET
                     VSEKMKQSIVSNFTEMSNQIIAKLQESKEQGIQSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQEHRTLTKEESAEINQIKTDMLNTAVVTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRAEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQASKVDWTTGEIKSK
                     WQVLNDTVKSKMQEWDSIAGDWVEGLKEKISTGWANLKENTAILWDNIKLTVTTKWTE
                     IKESISLKIEEIKQSISEKWESIKLFFTTTWESIKLIFSNAWDAIKQQLEEKFAPFIE
                     RLKIIFDGIKEFFRSTWEVIKNIVLGAVLLILDLVTGNFDKLKTDAIQIWENIKQSLS
                     DAWESIKEVFNNALAAIGDLLSNMWESMKSTAITIWESITQTLSSIWENIKSTCSTAW
                     EGLKTICSNTWENIKSTLANAWESMKTAVTTKLGEIKNNIVTTWNNVITFFKTLPAKL
                     KQAAIDMFTRLREGIDSKKQDAVNASQKIGTDVVNKIKEFPSKLLTAGQELVNGLVKG
                     ISGFAYKVVDEAKKLGQKAADAVRNALGIHSPSRVMIEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEADIKLFNKQIDELSKQEKAALKKVKGNAKDALKEE
                     YAAKKEKIKEAIELRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAKQASIKKELEALDRWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKILAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKMIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            34381..35247
                     /locus_tag="KPK72_000953"
     CDS             34381..35247
                     /locus_tag="KPK72_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBG2488195.1"
                     /translation="MQKLICRNSKGQEIKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPVSAAIYAKNKEDLELKRAYLTSLFNPRLGEMEFIYTNNALTR
                     RVKGVVQDITFQTPVGLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVINNGDIECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
     gene            35249..36328
                     /locus_tag="KPK72_000954"
     CDS             35249..36328
                     /locus_tag="KPK72_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HBG2488196.1"
                     /translation="MQQSIRILDKNINLIAEIDNYEELQIIKRFHKVGEFSLKINANK
                     NHVDKLVKNNIILLGKNYNKVCLIMHREFMYTETGEKSDVLFVKGVDLKGLLNRRLIV
                     PKTGEAFESHEGTQEEIIKAFVNNNCVTPSSSKRVIDNLIISENKNLGSADMWRSSYE
                     NLSDKIQEITEFCGLGWEITLDAKQKKFIFDVIAGKDLTINQTENPPIIFRSDFDNIR
                     ARHFTESIINSKNVVYAGAREDATKLVISVGEVDGFERNEVFADVSENTISILQKEGE
                     IKLKEYEELKSFELEIDPKNTFIYEKDYNLGDIVTIQDRKLKVTMDTRIVEVQESYSK
                     NGMKLKITFGSSIPTLFSKIKRMVR"
     gene            36328..37053
                     /locus_tag="KPK72_000955"
                     /pseudo
     CDS             36328..37053
                     /locus_tag="KPK72_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37027..37269
                     /locus_tag="KPK72_000956"
     CDS             37027..37269
                     /locus_tag="KPK72_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488197.1"
                     /translation="MNKVKEDLASIEITAQRATVEDAENLFEADSQGNKNVEIALNQL
                     AKHYNSLAEQQGNLEIEVNGQRAKGITIVNNLIDMI"
     gene            37286..38431
                     /locus_tag="KPK72_000957"
     CDS             37286..38431
                     /locus_tag="KPK72_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HBG2488198.1"
                     /translation="MTTKLEENAKLREMIDALETAKNDLQVGKNNIANILGLPFTGND
                     KLETTKNTLNSIRSTFVSNLNKKGVSTFANTPFKILAENVGKVEQGNMIVPPWYVPKN
                     VFIEALSAYAKNGIACVSAEKKIYFINGYAATSDSNNLCYDAILNTTTTKNTPSSFSK
                     SALIFYNGYIYKLGGFSGYSAPTTICQKYNILTNSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSVYKIVVFGL
                     RSSISAHYLTTINYDPVSGTSSKAGEGFDSNTFAVPLGSTLYVSDYGYKAGSGKCGVV
                     IFSDSGIKKLTFDKGTVPSYSYFGASVDGRQIYFILNGKITCFIPEL"
     gene            38450..38815
                     /locus_tag="KPK72_000958"
     CDS             38450..38815
                     /locus_tag="KPK72_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488199.1"
                     /translation="MIKIWDKKEKINGVEAEEILKSNYDFQTSEVFLILDDYERVTNI
                     ESVNTIKSIYKLDKSLTALETAEKYLEMHKKQEEEIKAHEELEKNSKNATIATHEEVK
                     ALREETAALTFAMIEKELI"
     gene            38816..38950
                     /locus_tag="KPK72_000959"
     CDS             38816..38950
                     /locus_tag="KPK72_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG2488200.1"
                     /translation="MWYKIIKKYYEDELWSKEQVKTAVEKNKITSTEYKEIVGEDYIV
                     "
BASE COUNT        16154 a         4341 c         6798 g        11672 t
ORIGIN      
        1 aagagttact aaaaagcttt atacttgttt attttgcttg tttttctatt aaaaataaaa
       61 ttatttatac tatatatact ttgtaaaaat tacttaaaat caatcgcttt tctattttaa
      121 aaagtaacca ttacttgaca aacagttact aaaaatagaa aggtgatatt tttatgatag
      181 aaaaattaaa cgaaagtgca agtttaagtc aattaataac cgcttttgaa aatagtacaa
      241 atgagattaa aacaattaaa gaaaatatag taaatgcagt aggaaatcca attacatcaa
      301 atgataaatt gagtgtaatt ccaaacaaat taaatgacat attatctgaa aaagataaaa
      361 ctataactca attaaatgct aagtacaagg ttgcaactgg aacagttact tcttttgcag
      421 atgatactaa aattgcttat ccatatcgaa ctgatagagc tgttaagcct ggtacttggg
      481 ttaaaattag taatttagat tttaaaccta atattttttt tgctgatttt gattactatg
      541 atattgaata taaaaataat tataaacttt tccttttcgc ttgtcgtggt gtcactacac
      601 aaggaggtgt tgatttttca agtgttacag cttttattag aaaaaatagt gatgagcatt
      661 ttcatgccaa aggatggctc tatggtaatt ccgaagggga tgtttatttt aataatgctg
      721 gtgttcaaat tccagcctat agctttaact caactcaaag acatatttat aaatggtatg
      781 ctattaaatt catttaaaag gaggaaaaaa catgaatgtt ccaaacagag taatatatga
      841 ccagacaggt agaataatct ttgaaacagg cgagtcctgc ggggatgtgt tgccacacga
      901 taaaataact gaactgcatt atatagatgt tgaatatgga agtatagatt atacaagaaa
      961 taagattgta ggcataaata tagaaacaaa agaacctgtc ttagaagaaa ttccagcata
     1021 tataagtaaa gcagaaaagg aaaaacaaga attagaaaat caattacttt taatgacaaa
     1081 taaagaacta ggtggaggta ttttataatg aatataaata atgttgtagt aagaatatta
     1141 gcagaaagaa ttttaaacaa aggactgaac cctttgaaaa acagaccttt tgagttagat
     1201 gacgtgacta acatagagta cagaaaagca gtagatgatt atataaatac aatagaacag
     1261 gaaataaaat agtaactttt agtaactctt cttaaagaat aggtcactaa aaaattttaa
     1321 ttttgtttat tttacttact ttcacatact tataaagaaa tcattctaaa ttctacatta
     1381 aaagaaacgt tcaaatttaa tcatctttct actttaaaag taacctattc tttaaaaatg
     1441 gttactaaaa atagaaaggt gatattttta tgatagaaaa attaaacgaa aatgctagtt
     1501 taagtgactt aataaccgct tttgaaaata gtacaaaaga attaaaagca agtaaagata
     1561 attttacaaa tttgctaggc aatccatttt tagaaagtac taaattctca gagtttgagg
     1621 gtaaaatgca aaatcttatg agtactttta aagataattt gaaaagtaaa ggcgtaagta
     1681 gcgggaatac agagtcttta ctatctttaa ttaataaagt tgcaagtata tatataccta
     1741 caccaatcta tacggcaagt ggtgaatgta acattagtta tagtggtgaa tgtaaaagtt
     1801 atacacttag tggctccagt agtgtttatt ttagtaaaat taacttgaat ttttatccaa
     1861 gatttatact tatctataat actggtggag ctagtaaata ttttacaact agctattttt
     1921 gcagttctgt cccttttata gcttctacag tcgaaagtta tactacttat ttattagata
     1981 gaagcgataa gcaagttcaa attcaaaaag gtgaatgtgt tttacctata tcttcttgta
     2041 ggtatgcaag tccatacttt tatttttatg ctataggtta aggagtgatt ttatgaatga
     2101 ttatatagag gtaggaagaa gaatattttt tgatgaagaa ggcgaaataa tattctatga
     2161 agggcaatcg aaaggaaatg ttcccgaaag aaaaaatatt aaaaaaatag aatatataga
     2221 tttggaatac gattatgttg attatgataa gtacaaaata ataggtataa atatagagac
     2281 taaacagcca attttagaag aaataccaat ctatatgagt gaagaagaaa agagaataca
     2341 agaattagaa aatcaattac taattgcaga aaatgaaaaa gtaggaggat tattataaag
     2401 aacacaataa tgttgtagta agaatattag ctaataaaaa atagcgaagt agtaaaagaa
     2461 gcagaaccgt ataaggttct ttttttatag aattagataa tttataagtt ttattttgat
     2521 aaaaaaacgg aggaaaatta tggaacaatt attaacagaa ttaagtagtt taggagcaat
     2581 aggcatatta tgcgctttgc tatttaaaaa taccatgcag gagaaaaaag aagatagaga
     2641 tatgtataaa aagacagtag aaaatttcat agaactatct acacagcaac aagagataaa
     2701 taagaatata cttgttgaaa tgggagcaat gaaaacagac gtagaagaaa tcaaagaaga
     2761 tgtaacagac ataaaagaca tgttacagaa agaaggtctt taaatgaaaa tagcactaac
     2821 agcagggcat acgctaacag gaaaaggaac aggagcaaca gggtatataa atgaaggaac
     2881 agaaaacagg atactaatgg aattgattgc taagtggctt aaaaaaggtg gtgctactgt
     2941 atatagtggt aaagtagata agtctaataa ctacttagca gaacaatgtc aaatagccaa
     3001 caagcaaaat gtagatgtag ctgtacagat acattttaat gcaaataaga caactctaaa
     3061 tcccatgggt acagagacaa tttacaaaac taataatggt aaagtatacg cggatagggt
     3121 aaatactaaa ttagcaactg tatttaaaaa cagaggtgca aaattagatg tcagagggct
     3181 ttactggctt agtcatacaa aagctccagc gatattaata gaagtgtgct tcgtagatag
     3241 caaagcagat acagactatt atattagaca taaagacata gttgctaagt taatagcaga
     3301 aggtatttta aataagacaa tagaaaatag tgaggataag aaaatgtata aacatacaat
     3361 cgtttatgat ggagaagttg acaaaatccc tgcaactgtg gttggttggg gttataatga
     3421 tgggaaaata ctgatatgtg atataaaaga ttatgtacca ggtaagacag aaaatttata
     3481 tgttgtagga ggtagagcat gtgagaagat tggttctatg actaaagaaa aatttactat
     3541 gataaagggt aatgatagat ttgatacact ttataaagca ttagatttta ttaagaaata
     3601 agtttgtaga gagtggtaac tatttcatca gttaccactc tctatttttt tatccttttt
     3661 gttatattct ctttcaagtg ctgttttttt aaaaacccat gtcgttccga attttttaca
     3721 gtctacacct tccacaaatt ttccatttct tatattaagt cttaacgtac tttcaccttt
     3781 tttataaagt tcacaagcat cttttataga gattagttcc tcaaatacat ctttcatttt
     3841 aacaactcct tttatttata ttataatcgc aaaagtttaa aaagacaaga aaaaaataaa
     3901 cgtttatgtt taaaaaacta ttgacttaat aaacgtaaac gtttataata tgattaaaga
     3961 aagataaaca aagggggatt taaaaatgaa aaaaataact aagaaagaaa ttaaaaagta
     4021 cgttagagaa gcggttaata acaattttaa ctgggaaata aataaatgtg ggttttacat
     4081 taaaaataat gaaatagaat tttttatttc ttacaagggt caaggaatag atgaaaatgt
     4141 atataatgat acttatgaag aaataattta cattgaggat attatagaag aatacagaag
     4201 aaaagaatac aatttagaag atgtagattc tattgtatat gacaatgtaa atgatatgat
     4261 taattgttat aatgaagaaa agtaataaaa ataaatacag tgagtttaat caaattcact
     4321 gtatttattt ttacaatagt agaataatat tattagttca ggatgtgata ggcttgaaaa
     4381 ttggagataa attcgaaaac cttacaatac tagacataga acaaaaaaac agtaggaaat
     4441 actgtctatg taaatgtaaa aattgtggca atgaaaagtg gataagagca gatagtttaa
     4501 aaagaattaa agggtgtgga tgtttgcaaa aagaaacaca gtttaagcaa aatgatttga
     4561 caaataaaaa gtttggaaga ttaacagcac taaaaaacac taataaaaaa gctaaaagtg
     4621 gtcactatat ttggatttgt aagtgctctt gtggaaatga gattaagaca acagaaaaca
     4681 atttaactac tggcagaact aaatcatgtg gatgtttgaa aaaggaatct aatataaaaa
     4741 atgcaaaggt agcattaaaa gtacataaag aaaaaaacat tattgatgat acaaatctat
     4801 ctattataaa aaagacagaa gcatatccta attcaaaaac taaaattaga ggagtttctt
     4861 ggaataaaga aaaaagaaaa tactgcgcac agatagaatt taagaaaaca cattataatt
     4921 taggatacta tgacaacata agagaagcag aagaagcata caaaaaagcc aaagaaaaat
     4981 tcttaaaaga aataaacgga aaagtttaaa aaaccattga cttaataaac gcaaacgttt
     5041 ataatataat taaagaaaga gataatcaag aggaggatat aaaaaatgaa taaacaaaaa
     5101 gctagaagat ttttaagagt tatagatatg aatatagata aaatagagga agaagctata
     5161 aaagctttta aagaaagttg tttaatcaaa gagactaata atataaaaat ttatatcgat
     5221 atacaaggaa aagttgaagc gatagcagtt caaacttggg ctaaactttt agatgataac
     5281 aaagaaatta atattttcac attaaatcaa acaccaactc atttaaacga tatgcttgga
     5341 gaaatttgtt acgtaaacga ttatgaagaa tttgaaaatt ggtgtgaaaa tgagtgggaa
     5401 aatttggatt gggatagtta taaaaaattc aataaagaaa atttcgaaga aattgctgaa
     5461 agaaatatag acgatagcac atcagttttt ttagaagaat tacaaaaagg cattgaaagt
     5521 tgtaaacaag aattgcaaaa tatagttgaa aattaagtag aagagtatta ttattaatgt
     5581 atatagtatc gcttttaaga ttaactacga gggatttaaa atatttttta atttatttag
     5641 aaatgatatt gtaataagaa tttttatatt aactacgagg gatttaaatg tcaatttaat
     5701 ttatagagaa ataatataat atcacattat tttatattaa ctacaaggga tttaaatatc
     5761 aatttaatct atatctatat gatataatat ccaattttag attaactata cggaaataaa
     5821 aacagtgtat tcaataaata cactgttttt ttaatgttta gttattacga taacttaatc
     5881 cttcaagtat aaaccctaca ttttccgtca tttcaaattc gctactttgc aatattcctt
     5941 ccgtaaattt gctgtcattt ggtaatgtta tttttttcct tgccaccgct tctatgattg
     6001 tttggaatag tttaattttc atatccttat caactccagc atatcgcaaa aaatttacaa
     6061 cttcttgtga aaacttattt aattcagatt tagtcatttt ttcggataat tcttttccga
     6121 tttgcagata aacgttcatt ttttccaacc cctttttaga ttatagttaa aacatattat
     6181 ttaaacaata ttaatatatt gtaataataa tattaaaata ttacttaaat attatatata
     6241 caatatttaa gtaatatcta aacaatataa atataatatt atttactttt tcttagtctt
     6301 atttcttcta tttctctcat tgtatatatt taaattctct tcttttatag tgacatcaga
     6361 taaaatttga gttagtatat cacttaataa tttgctaacg tcgctgttca tatcaataga
     6421 aaaagatttt aatttgttta caagttcttc atcaagcgtt gcactcatag ttactttttt
     6481 acttttctta ttaactagat tatatatgtt taatgtctta ttgtctgtag ttgtagaatc
     6541 attttgtatg attgttccat ttcttttgaa aactggtttt tctttattag ataaaagttc
     6601 tccgtcgaat tttggcatct ataacactcc tttcactttt agttcttcta acatattagc
     6661 aaactcttgt ctgctgaaat gctctttagt tatttttgta taatctgtta gagatagttt
     6721 tttcaatagt gccttctcta taaactcgtt ttttctaata tatgtatcta acataatatc
     6781 tctcatatcg ttaaaccctt ccagataaga atcgaaagta tcactaattt gagttttctt
     6841 cttctcaaat ccaaccaaaa cagtactttt tatattatcc tctttatcaa aataggttct
     6901 atcaacatcc cagagctgtt taaataattc tgctcctctt aaagaagaaa tgtttttatc
     6961 ttgaattaga ataattatac tatctgctaa gaaaagtaca tttttagctg tcaaatcgta
     7021 gcgtggagat aaatcacaga taatataatc atactcactc aaagtattaa agttttgcat
     7081 gtaccagcga gctaaaaatt tctctcttgc tggtaaaggt gatagctctt gttcaaatct
     7141 agccatttgt atgtcggaag gaattaagtc tatattagtg taatttttat taggcgactt
     7201 gactattgca tcattagcgc taatcccttt taaaatatct aatgtagtat tatcattatg
     7261 atttatttca taaacatatt gtgttagatt tgcttgtaca tctaagtccc ataagagtat
     7321 ttttttatct ttatcttttg caagttcata tgctgtcatt atagacgttg ttgtcttata
     7381 tattcctcct tttatgttaa agtacgtaag tagctttgta ttagccattt ttctccacct
     7441 cattattata tttataatat tataataata tcatagtatt gtttttataa tattacaaca
     7501 atatattaat attattaatt taaatattta aataatataa atacaatatg taaatattgc
     7561 attcataata ttaatatatt atattcacaa tattaaaata ttgtatatgc aatactaaaa
     7621 taatattttt ataatattac agtattattt ttatattata gtaaaattta aaaaagaagg
     7681 ctaagaaaaa agcaaccttc tttttataga aaaagtcatt cattcttagc cttttcaatt
     7741 attatcctgt cgccctcgaa tgttgccgta acattagggt tttctcttgt tatgcccatt
     7801 tcatcggccc attttttggg aacactcatt ctaggagtaa acgacccact cccaccttta
     7861 tgaaagttaa actttaaatc tcttttttct tttttcattc attagctcct tttttgctgt
     7921 attacaaaga aaagtaataa tacaacacta aaaactaaac cgagtatgtt gcttgtacta
     7981 gagtttaata ctatagatac tatatttaaa actatagtta ttaaaactaa aacaatacaa
     8041 atcttataaa atttatcact catgtttttt ttagaaatgt gttataatat tagaaagaag
     8101 tcacctagag agaagggcta ttctctctag gcttatcctt acaactcttt taaaattctt
     8161 agaattgcaa ggattatgtt aagtaataat aagatgaagg agaaaacttt tttaactatg
     8221 ttaaatttgt tttctctttt ctttttactt ctttctaatc tccccattcc tgtgcctcct
     8281 ttcttttatt tctttttact ttctcacctc cttctctata aatatattat atcatgacgg
     8341 ggcgtcaaag gcaatagttt tctcctattt tttaaacaaa ttttggtaaa aagtaaagat
     8401 tttagtatat tagaaatgat gtaaaattgt atctttttta aaaaatgtga tataataaaa
     8461 gcaaggaaat aatgatttac tttacacaag agtagctatt tccaatcaga ttaaaaataa
     8521 cttattttct taaaccactc ttattggcgt ttgagtgttt tttaatttta tcgttcttaa
     8581 aataaacttt gataaaaata aaaaaataga atactgaaaa tgatgtaaag ttgtatcttt
     8641 taaagaaaat gtgatataat aaaagcaaga gaactgtatt ctagtcgtaa gagtagagtt
     8701 ttcgtctaaa gtaaaaaaat tatctagact ttttgaattt gatttttaaa tcaaattccc
     8761 aaccactctt ttgcgcagag tggtttttta cttgtctaaa taatttacta attatataga
     8821 atattaaact agctataaca tcagctaata tgttaagtaa aaaattatcc atacatattc
     8881 acctcccctc atagcgttgg gaggataatc ttttgtgtat agactccact ctttttttag
     8941 aatatagatt gcttctcttg ctaaaattat tataacatat atttacataa aaaaagactt
     9001 agtatatata ctaagtcttt taaaatataa tttattttca aaatcaatat atttttttat
     9061 tttatattta tgctataatt aatattaact tattacagat tacaaaaaca aataaaaaag
     9121 gaataagcac caagtcgcca aactaacact tattccttac cacggagctt gaagctctta
     9181 taagatataa ttatatcttt ttttaactaa tttataagtt atattataga ttaaaacaac
     9241 ttataaatca atgttttaga gttaaaaata atttgaaatt tttaacttaa ttagttaaaa
     9301 gtgcttcaag ccccgtttag aagggggaaa taaaatgtct ttagaagaag caaaagaatt
     9361 agaaaaaaat tataaattac caatgccaac tttttgtttg aaagaaaaag gatattttgc
     9421 aagagaatac gccgccttga tgttattaag taacggagga atgaaaatta tgcatacaga
     9481 aaaaaataga tacttatatc aaaataaatt cgatatagat gcattagcaa aacaactaag
     9541 catttcaaga gctacattat acagaaatat taaaaaatta gaagaactta aatgtggtat
     9601 tctaaaaata gaaaatacga aaaatggaac tgtatacaga ctaaaatatg gaattgaaac
     9661 aggttataat gacgaagtgc ataagtttgt tacaatacat cataaaatac ttgaagaatt
     9721 agtaatcaat ttcaaaactc caagcgtaaa actttattgt cttttatgtt atttaacaaa
     9781 tgaaatagat tttaaagtta tagacgaaga atttttatgt aataatattg gtctttgtgg
     9841 caagagcgaa agtaataaaa acaaagttag aagtatgata cgagtattaa tgaaatgcaa
     9901 atacatagac gtaaaaaaag agaatagaac tttctggaat gaagaaacaa aaaataaaat
     9961 taataaaaca ctaaaattat atagattatg tagttacgaa gagtggaaaa acttaaatga
    10021 taaaacagac tttaaagatt aaggtctgtt ttttttgtgg ataactatgt taataacctg
    10081 ttgataaaat gtggacaact tattgttttt taattagaaa tctaacttgt ctcaaaaagt
    10141 ggtgggtgca ttctcaaaaa gtggtggtcg cattctcaaa aagtggtggt ctaaatatat
    10201 agagaactta ataaatagaa aacataaata tgttttctat ttatttcttt atttaagaaa
    10261 atcgaaaact taaaaatttt taatctatgt aattagaaat ctaaccggat gcaaaaagtg
    10321 gtggtcgcta tttaaaatgt tattagcatt tttcaaaagt aaagaaactc ttagaatata
    10381 tcttttgata aaaagaaaat gattatttga ttaagaaact agaatatcat ttagaagcga
    10441 tacaagacac cagaaacaat ttttctattt gctataaata gtttatgtta aatagcataa
    10501 aaatattctt aaaatggaaa taaataggta ttgctctttt gaatcatttt tatactacta
    10561 gaatagaaaa ttaaaggaat ataagttttg atgttgaaat atataacaaa acataaaaat
    10621 acggaggatt tgaggatgaa aaaagtatta ttattaatta cagtatgttt attaagcgta
    10681 gggttggttg cttgttcatc aactaataac aaagatgctg gaaacacaac taaaaaagaa
    10741 aagaaagcaa acgaaaatga ggttgatgga attaaaataa ctataaaaaa tgttacaaaa
    10801 gaagatataa aaggagatat aaacgaagac ggaagtttta acgaaaatgg agaatatttt
    10861 agtgatggaa tcgaaaagaa aaaggcagct gattatgtgt acgaagttgt aaatgtagaa
    10921 gtagaaaata aaacagataa agcggttaaa ttatttcaaa caggatggaa cgcagtcggt
    10981 acagatggtt acgaatttaa agatataaaa gcaactgata aattagacaa tcaacaagta
    11041 ccagctaact acaaatttga tgcacaagtt aaaatattag ttgaaaagaa tatgaatgta
    11101 aaagaaatag ttttaaaata taatctaaaa gattattcaa gactttttga agcaatggag
    11161 tatgcaaatc aaggagcgtc aaaatcagat gtagagaaaa agtttccaga gctatacaag
    11221 gataattgga ttgaattagg agaaattaaa gtagagcagt aaaaaaaagg gctaggtata
    11281 agcaattacc tagtccttat tttatattct tttaaaaaat ctctactaag tgttttaact
    11341 gcttttttat tatatttttt agtgtttttt aaatgctccc tttctttttc taaaaaaaaa
    11401 tcagataaaa ccaattggtc aatttctaat acatcagcta gtaaaataat ttgagttaga
    11461 gttggactat gcttaaattt ttcattttcc attttcgaaa tgtaactttg acttatacct
    11521 gtcaattcac ttaatcgacg ttgagtgagt tttttctttt ttctatgata ttttaataat
    11581 gtatccatag tagaaattaa actataatgt taaaaaacat taaaatcaat aggaaaaaat
    11641 tacatttcac agaatgataa aattaattgg taaaaattgt aaaatgaggt cggaatattc
    11701 gtcgtacgaa tattttttac atacgttttt atgctactat tgtagtaaag atatgaagcg
    11761 catcaaatct ttaggaaaac aatttcaagt actattaatc aatattagta aatgccaggg
    11821 ggtaatcatg aaaaaatcac aaattaaaac aaaaaacaga tacgttgaaa ataagataga
    11881 aacagctatt tgtgtaaatg aattgaaata tatttctgtt gatgattatg ttttcttaaa
    11941 agaatgtata gaaaaaattt atataaaaaa taaaaggact tgatttttat caagtccttt
    12001 tattttttaa tttagattaa ttatttttct tgtgtaaaag ctctatcatt tgtaaaatag
    12061 catttctatc ttcatattct aactcataga gcttattagt tatctctttt acatcttcat
    12121 caatttccag ttcagataaa caatccttat aaatatcacc aatccctttt cttagccatt
    12181 cttcatttac atcaaattcc ctacaaatat cattgatact tctttctgtt agtactcttt
    12241 ttcctttttc caataaagaa atctggtctt gtgataagaa aattttttct ccaaaagcct
    12301 tttgtgaaag tttttcgttt gttcttaata aatttaacct tttgccaatt tctttgttta
    12361 ttgtttcctt agaaatttca tccatttttt aatcctcctt ttataattat tgtatcataa
    12421 aaatacgact ttgtcatttg ttaagaagaa tttacgacaa aatcataaaa attataaaaa
    12481 aagcattgca ttttaacgac aaagtagtta taattaaatt aagcttaacg acaaagtaaa
    12541 aaaaggaggt acgaaatggt ttattctagc aaagaaaaaa tagaaacagc tctaatgata
    12601 gaatttttaa aaaaaaacga ccctaggaat tgggaatcta tagaaactct aatatcaagt
    12661 acttacacat taagtagctt taaaaaagga aataaaatat ctcaaaaaag aggtgataaa
    12721 attgaacttt aataaatatc aaaaaggtga aattgttcaa ttatacgaaa caaaattcag
    12781 ttgcaataga aattgcattg ttacatatgc agatgaaaaa gaaatagtat tgatgtacta
    12841 cgacaaagaa ataaaagaat taatatataa aactttaact aaagaagatt tgatatttaa
    12901 tgactatgaa cttaagttac tatcttaata aataatttat aaacaaagga ggtgagggat
    12961 tggaactttt aaaagattca agaaaaagag attggttttg ggtagaaaac gatttaattg
    13021 acagagaaga tttgactatt tacgaaaaaa tgatttatat gacattagtt aggcatttaa
    13081 acgaaaatag tttttgcttt ccaagtcaca aaactatttc taaaaaaagt ggatgtagtg
    13141 aaagtcaagt caagaaagtt ataaaaagct tagaaaataa agaacttata agaaaagaaa
    13201 atagaaccaa attaaattct aaagaaaaag atagtaatat ttattttgta ttgactgcaa
    13261 aaataagtag ggcatatggt gacctacccg taggtcacag tgaaacccac cctagggcat
    13321 gtggtgacct acccgtgggg catgtggtga ctagtaacaa gactaatatt aacaagacta
    13381 atttaactaa taatatatat agttctgcag agcaaaaaga agaaaagaca aaagacaaag
    13441 agatatataa aagaatcgtt gattacttaa ataaaacagc agataaatct tttaagagta
    13501 caacaaagaa aactatatct ttgattgatg caagactcaa agaaggcttt acagaagaag
    13561 aattttacaa ggtcatagat aacaaagtgt taaattggct agataatgat aaaatgaacg
    13621 cttatttacg ccctgagacg ttgtttggaa acaaattcga gtcttattta aacgagacac
    13681 ctaaaaagtc tctaaaaggc aatgagagcg tcaaaggaaa aacaaagaca aataataata
    13741 aatttgctaa tttcaatcaa acatttgata aatattcaga agatgaactt gatgcaatta
    13801 taaagaaaag tcaaagtgct aaatttaaat aaaattaaaa aaggtggggt aaaaaaatgg
    13861 aaattaatag cgtaagagag ttaagagaat tgtttgaact ttttacagtt ataaaagcgg
    13921 aaggcaacaa tgttgtttct tgcaatgaaa ctgtaaacga gttaagtaac aaagaactaa
    13981 taaaagcaaa tgaaaacaaa tttaaaaaac cttggaatga tgctctatta tatgaaataa
    14041 atgctttaga tataataaaa aagcataagc acagtgatag aaaagaaaag gcatataaaa
    14101 cttatctagg attagaaaat gcagatatag aagaaattta taaaattcat tatggagata
    14161 aagaaaattc tgaaactgtt gcaagttgcg aagaaattag ttcagcaaaa acggatacaa
    14221 tatcttgcaa tcatgacagt gtttcaaaag gtaaagatac tagtgattca gaatgtactt
    14281 gttttatttg ttctaaaaaa atagaaaatc aagaagatgt atttaaaata ccttttttaa
    14341 cacgagatta tttgagttca gcaaaagaaa taaaaaatat tgaaatttgt gagaaatgtt
    14401 tggatggtaa ttcgtggagt attataagaa gaaagcagtt taagaaaatg aagaaataaa
    14461 tatttaaatt agtatcttta catgtgaaaa aggagagtga aaaaatgata aaaaataatg
    14521 ttttaagtta tgaagaaaaa ataaaagtat taaaagagca tggctataat gattctacca
    14581 aggaatatat tttaaaagaa tttggcaagg agcatatatt ttatgaatca tttttaagag
    14641 caatagaaag agatgacagc aaaaaaagtg tactaattaa aaaatttgaa aaaggtcagc
    14701 accaattttc agaaggtttt ataaaaacac acactatcag tgaactacta aaagaagaag
    14761 aacgtattag aaaaatacta ggataaggct tagaaatatc tataatctaa accttatatt
    14821 taaaactatt aaaacttacc taatatgtac tctattgcga taggtagaga catatttaaa
    14881 aaacttgagg tggaactttt tattttattc catttagtgc tgtctcgtat ttcatctaaa
    14941 tattgatagc cttttaaaga caactgcgtg acctctaaaa cagtatcatc accataatta
    15001 acatcatcaa cgagactgtt ttgataaatt aaatttatat gatgaataat agttgattca
    15061 tcatatttga gattatctag tatagtatca aattgtaata caggagaaat gggagttgta
    15121 ttagattcaa taaataataa aatatctctt atacaatctt ggttagtaac catagtatca
    15181 cctccttttg ctttagtata gcacaggaga aatgagatga taataaattt tactgtagca
    15241 gaatgagaag gagcaaagca aatgcaaaag agaattacag aaatgagtat attagaaaaa
    15301 gttgatttaa ttggacagaa acaaaagaaa ctacaagaaa aatataaaaa tctaagtaag
    15361 ggagaattgt tttctattgc ttgtaatgaa gttaaaaaaa tagaagagca ggagggaaaa
    15421 tattttgaat ataggtagtt atacattagt taagaaacaa ctaattgaag aattgaaaga
    15481 agaaattaag caaaaagaag aaaacttaaa agaacgtgag aaatacataa aagaattaaa
    15541 agagaggtta gaatgttttg cgagggatgc gttagaatca gaagagtgca taatagatat
    15601 tgaaaatatt ttgatagaac aaactgtaga attagaagct tataatttat tcatagattg
    15661 cattttaaac aaagatataa gaaaagctat tgcgatttat aacagaacta aaagcatcag
    15721 aattaagcat aaaaatatta attctatatc taagaaaatt gaaaaaataa aacaaataat
    15781 ttccttgtag aaataggagg agtttaatga tggataagtt tgtaacatgc gctagatgtg
    15841 aaagaacaat aaatgtagaa gaaaataatc atgtgaagta tgaagaagaa actttaaatt
    15901 taagttttac gttgtatttc tgtttaggct gcgtagataa attaattgaa caatctaaaa
    15961 tgttggaggg agaaaatgaa taaaattagc gttacacaag cattagagaa atttgacagt
    16021 ttactagata attggaacga ccttccaaat catgtttaca cacttgaata tagaagcaaa
    16081 ttttacgaat ggataaagtt gcttgaaaga aaaggtagtt tacaaaatta taagatagtt
    16141 gaagttttaa atgctgaaag aaatggagaa gaagcgtcgt tttggaattg aagaaatata
    16201 acttgaaaag gacttaaact ttttctagat agaagggagg tatataaata tgaaaatgat
    16261 aaatgttttt aaattatcat caatgtattg tatttgcccc aaatgcggga gcgataaact
    16321 tggagaaggt gaaggaaagt taattgttga tgaacatact tattacaggg aatgtaaatg
    16381 tggttttaat gttcttgtag atgaaagaga agacgaaata taaggaaggt gctggaaatg
    16441 aatagtgtta ttttaattgg aagattaaca aaagacccag aacttaaata tataccgggt
    16501 tctggaacag ctgtatcgac ttttacaata gcagtagata gagattatat taaaaaagat
    16561 ggaactaaag aaactgattt tatacctatt gaggtgatgg ggaagttagc agaagtatgt
    16621 gctaataatt taggtaaagg taggttagta gcggttgaag ggtctataaa agttaatagt
    16681 tatgaaaaag acggggagaa aagaacttat acaaaagttc atgctaataa gattaagttt
    16741 ttagactata aaaaagatga tactgaaaaa gaatatatgt ttgaaccgaa aggtttagac
    16801 caacaaggat ttcaagctat agatgaccca gatataccat tttaaagcgg gagggataat
    16861 aatgtctaat ataaataaaa aaacagctaa aatttgcggt tggtgtggaa caacagtcta
    16921 ctcttatgta gatggtaaaa ttgcatattg ttctaagaaa tgtaagaaaa aaagagaaaa
    16981 aaatttagaa atagaagggg cgaaaaaatg aagtatattg caagttttag tggaggaaaa
    17041 gattcagcag caatgctcct tctaatatta gaaaagaaat tacctcttga tgaaattgta
    17101 tttatagata caggactaga atttgaagaa atttataaca taatagatga ttttgaaaaa
    17161 agaataaact ttaaaataac aagaattaaa gcagaaaaaa cctttgaaga atacttttat
    17221 actgttaata aacaaggtaa gcgtaaaggg caaatatggg gttttcctta tcttttaggg
    17281 gcatggtaaa agtgaaatat ctgaatatat agaaaaaatt ggtggcaaac aattaaattt
    17341 tataaaaaga agagatagtg acttaacagt tgaaagttgc aggagaataa aataaggaga
    17401 aaaaataaaa tgaaaaaaac aaaaatcgaa gaatttatta ctaaagttaa ggaaagtatg
    17461 caaagtgtag caaaagaaag tataaaagac ttagaaaata ttttgaaaga aattgaaact
    17521 ggagaaacag aagttaaaat tttatttatt gacaaggaaa aaaaagttga agagattgtt
    17581 gaatttacac atgacgaata cgtgaaaatg attattgaaa aaatgatatt tgatagtctt
    17641 aaaataataa aagaacaaca cgaaaagatt ttaaaagagt tcgaagaaag agaggtctaa
    17701 aaatgaattt taaaatgatt gcaaatatac caaagtggac agtaaaagaa caatttgaaa
    17761 aagtgcttga agaagtttta gaacttaaag aagctattga attaaaagat aacaaaaaaa
    17821 taatggaaga aggtttggat gttattcaag caatttttac actatttaaa gtattaaaga
    17881 ttgataaaga aatacgcgaa ggtttgaagc tacataataa aaaactaaga aaaagaaaat
    17941 ggaaactcga aaaaataaat tagcataggg gggagttaaa atgagtatgt gtagaaaatg
    18001 tttgtgcgga gactatttag atgaactagt agtaggcgaa tgtattttat gtggtcaaga
    18061 attttttttc tataaaaact ccagttggga aaatgtgtcc tagctgttca gaaatgttca
    18121 aggggaattg ttctcaatgt gcaaaaccaa ttggaaatga aaaaaagtta tttaaaaata
    18181 aattaaagta aataggcaat aaaaaacgca attagattaa tttttaaatt aaaatagata
    18241 aaacgcttta taaatataga ttttatttaa acttaaaaaa atgaagtctt ataacaaaaa
    18301 aacaaaataa aaatacagaa tattttgaat aatatgcagt agttggaaat gctacaaaat
    18361 acctaactaa tcctaaaagc taaatttaag gattagttag gtatttataa caaaaagtac
    18421 aagaggtgta aaaatgaaaa taaaaaaaga gtattttgac aaagtggaaa atgcattaaa
    18481 aatgttagga gataaatata gaaagttaag agtattaaaa aaagaaatag caattcttaa
    18541 acacaaagaa aactatagag aaattaatta tgaagaatta gggtttaaag tacaaaaaag
    18601 tgtaaaaggt atagatgaca tggttgtaac aatagaggat gctatctaca acaaagaaac
    18661 tgaaattgaa attatagagc gaaagctaga gttttataat atttatttaa aagagctatc
    18721 agagatagaa caaaaaatta tagaactagt atacttttat agctggaatg ggaaaatgcc
    18781 tattacaaaa atggcgatgg aattaaatta tgatagaact agtctttata acaaaaaagc
    18841 tattgctatt aataaaatag ctttaatgat atatggtgat gaagctttag aataaacatt
    18901 atttttacag aaaaaagact atttttatac ttcgtttcta aagaaagcat gttagaattg
    18961 tatcatagaa aaaagtgtac attgacaact gaatatcccc cgaaacctaa agtctaaact
    19021 tgaagctcga aagtttaggc ttttttattg ctaaaaaagg aagggggtga gtaaatgaat
    19081 tttgtagaac ctatcagaaa tttagagaag ctagaaagca tatgcgctta tttagaagaa
    19141 actaacatga gaaacaatgt actcttcagt atgggcatat atacaggact tagagtaggg
    19201 gacatattaa agcttagaat aaaagatgtt catgacaaac ggtttataac tcttaaagaa
    19261 agtaagacgg gtaagactaa gcctatcgag gttaatccta ttttgagaag aatactaaaa
    19321 gaatatacaa aagataaaaa tcaaaatgaa tttttaatta agagtagaga gcagaccaat
    19381 aaagctataa gcagagtcat ggcttataag ataatacgag aagtaggcga aatgtttggt
    19441 atagataatc taggcacaca tacaatgcgt aaaacttttg gttatcatta ctataaaaga
    19501 acacatgatg ttgtaacatt gcagaagctt tttaatcata gttcagctac gataaccttg
    19561 cggtatattg gaattgaaca ggatgattta aacaaagcat ataggaatat taaatacttt
    19621 tagcttaaga gtttaacata aaaaggtcac gttaaactgg ataaaatata gtctaataat
    19681 aaagtcttga atgtattgtt aatactaaag tgtaaggctg tttgataata cttaaatgag
    19741 tttaacagaa tatatcttat gttaaactca actaggaaat taaaggagta tcactatgaa
    19801 taagtatggt aagtttaaat gtaataattg cggaaagaaa gttaatgtaa ataatagaga
    19861 tttaaagact aaagttattg ataatactac tgttacatac ttcatgtgtc gcagatgctt
    19921 tgagaagttt gttgtatcat gtgaggatga ttatattaaa cataagagag aagaatataa
    19981 gaagcttaaa gatgatgaag tcaagctatt gcaagacatg aaagagtata gcgacggtct
    20041 aggagagctg tatattaatg agttataagc acatgacact agatagatta aaagaattaa
    20101 ttttaattga tagacttgat atattctata gaagtacaga ctggcgtaaa ttaagaaaaa
    20161 aagcacttga aagagataat aatgaatgtc agatttgtaa agaaaaaaag aaagtatcta
    20221 aggcggaatg tgtacatcat atcaaagagg taaaagagta tccaggtgaa gctttgaagc
    20281 ttaataacct aacaagcttg tgcaaccttt gtcacaacga aattcacgaa aaaatactaa
    20341 aaattaatca agaaaaaaag aaaaaatttt tcagtgaaga gcgatggtag atagcccccc
    20401 tattgaaaaa actggctttc aaggcaaaat tttgtgaacc gaggtaggta catggctccg
    20461 caaggagacg tcaatttaac aaaaggtgtg gggggtgggg gtcttgcaac caaaattatg
    20521 gttgcgggtg cagaaaaaaa cataaaaatg aggtggtgaa aatggatgat aatgaggaat
    20581 tagaaattag gcgagacgta cagaatgact acgttttagg catgacttac aaagaaatgt
    20641 cagccaagta taacataccc gaaaacactt tgaaaagttg gagaaaaaga gggggttgga
    20701 ctcgcaactg caccccaaaa aagggttgcg tgaaaaatgg gttgcaagag tcgggaaata
    20761 atttaaaaaa ctctataaga aaagatttat taagacaaat aaaagagaat aacatgacag
    20821 aagaatactt caaagactta gttgaagatt atttaaaaat gtgggatata aaaaatgaat
    20881 taatagaaga tgtaaaaact agaggtgttc aagtcaaata taaaaatggt caaaatcaat
    20941 ggggctataa aaaaaacgat agtgtatcag agcttattaa gtataacgct caaatgttaa
    21001 gtttacttga taaactaggt ataaaagcga caccaaaagt tttgggtgat gatgatattg
    21061 aattataagt acaataaata catagatgaa tatttcaact tagttgataa aggcgaaatt
    21121 gttctatgta aagaaatgga acttgcagtt aagaaaataa aaaaagattt aaaaaaacct
    21181 aatatctata tcgaccatga aaaagttgaa aaagctatcg aatacatgga aaagtatttc
    21241 tataaaatgt atgcttggga aaaatttgtt gttgctttag tagttggctg ctttgaaggc
    21301 gacgaacttt tatatgatga aatttttatc atgatgggca ggggaaatgg aaaaaatggt
    21361 tttatatctc aattagctaa ttatttgcaa acacatttac acggtgtaaa aaattatggt
    21421 attgatattg ttgcaatgtc agaagaacaa gcaaaaacaa gttttgatga tgtttataac
    21481 atgttatctg aacatcctgc tatatgcaaa aagtttttta acagaactaa agaagtaata
    21541 acatttaaaa aaactaacag ctatttaaag ttccgcacaa ataatgctaa gtcaaaggat
    21601 ggattaagac ccggctgcat catttttgat gaggttcatg catacgaaaa ttatgataat
    21661 ataaaagttt ttacctctgc acttggaaaa gtaaaaaatc ctagggtttt ttacattaca
    21721 actgatggaa atgtaagagg tggggtatta gacgatttca aagaggaatc aaaacaaatt
    21781 ttaaatgaag aaattaaaaa ctctaaaatg ctgccactta tttttaagct tgatagtata
    21841 gaagaaatgg aaaatattga tttactggaa aaagcaaacc cgagcataaa gtataattca
    21901 gacttaagga cacaaatttt aaaagacatt gacaaagcta aaaatagacc gcagatgatg
    21961 atagaattac taactaaaag atttaatttg ccagcgcaaa atttagcgac tgttgttgct
    22021 gaatgggatg acatagttgc aacaaacgaa gaattaccag acctaaaagg cttttcgtgc
    22081 attggaggat tagatttttc tagtataaga gattttacaa gcgtggtatt gttatttaaa
    22141 aaagataaaa aacgatatat catgcatcat acttttatat gttataagtc tttagaaata
    22201 acaaatttta aatttgatat tgaattagca aagcaaaaag gtctttgtac aataatttat
    22261 gatgaaacga taaaagcaga gtatattgct gattggttca ttgaaaaagc ggaggattat
    22321 aatatattaa aaatctgctg tgactcatat agagctgcac atgtaaaaga agaatttgct
    22381 aacaaaggtt taccaatcga agaggttaga aatggttcaa taactcatgc gaaaatagcc
    22441 cctcttgtag aacaaatctt cgcagagaga acaattaaat ggggcgacga catgatgatg
    22501 cgttggtata ctaataatgt ttatgtagat acagacaaaa aaggaaataa aactttttta
    22561 aagatagagc caatcaaaag gaaaacggat ggcttttttg catttttaca tgctttatca
    22621 aaagatggtg aactgatgca agaatcaacg attaagtttt atgattgtta tacatattaa
    22681 aaataatgag gtggtgaatt gaattttaaa aattttataa ctaatttttt atttaaagat
    22741 aaaatgtctg gagcagatgg agagtatttt aaaaaactag aagctacgat attttataaa
    22801 gaatttgcat taaggtcatg tatttctatt attgcaaatg ctttagtttt aagtgaattt
    22861 cagacgttta taaattcaaa gtctgttaag gaaaaaaatt attacttgtt taatatagaa
    22921 cctaatcaaa atcaaaacgc aacagaattt tggcaagaag taattacaag acttgtgtat
    22981 gacaatgaat gtctagttat acaagaaaac gaacaacttt tcgtagcaga aagttttaat
    23041 gtagataagt atgtttttta tgaagatgta tacaaaaata ttacaattag aaactataaa
    23101 ttagatagaa gctttagaga gtcagacgtt ttatatttta aattaaatgt tgaaagcata
    23161 aaaactgtaa tagattatct ttataatgat tataaagatt tattaagtag cgccatgaag
    23221 ggttataaaa aaaataatag cgaaaagggc gtacttgaaa tagacacaaa ttacccacag
    23281 acaaaagaag cacaagaacg acttaatgat ttaatgagta ataagtttaa acgttatttt
    23341 gaaagtgata atgctgtttt accgctttcg aatggattaa aatatacaga aaatacaaaa
    23401 gcttcaagta taaaagatag cagagatata agagcgatta ttgatgatgt tatggacttt
    23461 gtatgcgctg cttttcatat tcctgctggg cttataaaag gtaatgtttc tggcgttgaa
    23521 ggtataacag ataatttttt aaacttttcc gtcaatccaa tcgctcgact tataacaact
    23581 gaaataacta gaaaaatgta tggtagagat aaattttttg aaaagagtta tgtaaaagta
    23641 gatacacaaa aaattgctaa catgggactt gaaaaaatat caaaagcaag tgacttactg
    23701 tttagaatag gtgtaaattc tataaatgac aatttagaaa tgcttggtcg agaaaaaata
    23761 aatgagtctt gggctgatga acattatgta actaaaaact atcagtcgat tttaaatccg
    23821 gatttgaaag gaggggctaa caatggaaat ggagaaaaga gcggtatcgc tggaaataag
    23881 ggacagtaat ttagaaagtc gtaagataag tggttatgct gctattttta atgatgatta
    23941 tactaaattg caagacagat ggggcgatac tttttacgaa aagatttcaa gaggtgcatt
    24001 tttaaaaact ttagcagata acactaggga taagtttatg cttataaatc atgactggaa
    24061 taaagttata ggtagaacta actcaaatct aattttagaa gaagatgaac gtgggttaag
    24121 gtttgaacta gatataccag ctacgacgga cgggaatgat ttgcttgaaa atgtaagact
    24181 tggactaatt aaagggtgtt cttttggttt taatattgta aattcaaaaa ctcgctttga
    24241 tgatgattgg acatattaca gagatataac agaagttgaa ctatttgaaa ttacagcaac
    24301 ccctattcct gcttataatg atactgaaat aaattgtaga agtgatatat ctataaaaga
    24361 acttagagaa tctcaaaaag aaaaagtaaa acaaaatgat aataaagata atatagaaaa
    24421 aaggaatgtc gacttagtat cggctttttt taatgcattt aatcgaggag gaaaataaaa
    24481 aatggcaatt aaaaatttag atgaggcaac taatgttgaa ctaagaacga atttaatgga
    24541 agcgataaag agtaatgacg aagaaaaaat aacaacatca tttttaagaa tggcggaaga
    24601 tatacaaagt aatttactaa aagaagcacg ttctattgct aatatagaat cagctgacag
    24661 ggcgatactt gacaagaggg gtatgccaca attaacaagc gaggaaagag catattatag
    24721 ccaagtgata gaaaaaagag gatttactga tttagagata actatgccgc gcactatatt
    24781 tgatagagtg tttgatgact tagaacaaaa ccacccccta cttagtgcaa taacttttga
    24841 aaatacgaca ggcgttacgg agtgggtaat aagaaaaacg ggaacggagg cagcttggtg
    24901 ggggcaatta actgacccta ttaaaaaaga gttagaaggt ggatttgcaa aaattaatac
    24961 tgttgcttat aaattatctt cttatcttcc agttgcaaaa tctatgcttg acctaggtgc
    25021 tgaatggtta gacagatatg taagagtagt tttgactgaa tctatatcat tggggcttga
    25081 aatgggaata gtagcaggaa caggaaaaga ccaacctatt gggatgatga aggacttaaa
    25141 aggctctgtt gttgaaggtg tttatccaga taaaaccgca aaaccattag ctgattttaa
    25201 accaaataca ttaggaactg aaatcatggc accacttaca aatggtggta aaaggacagt
    25261 cacaagcgtt ataatgcttg taaatccggt tgattattgg gctaaaatct tcggtgcaac
    25321 gactatgcta acttcaaatg gcgcttatgt atatggagtt ctgcctatac caggaaatat
    25381 aattcagact gttgctgttc caaaaaataa attaatcgta ggagttgcta aagattattt
    25441 tatgggaatt gggtcagctg gtaaaataga gtattcagat gaatataagt tcttagaaga
    25501 tgtaagagtc tatcttgcta agcagtacgc aactggaaca ccaaaggata atttttcttt
    25561 tttagtattt gatatagaaa acttagacac aactttagca acagaaatta attcaaacac
    25621 aaaaacgtca gctactagag caaaataggt gaaaaatatg gaaaatgaaa atattaaaag
    25681 tatagttgaa gaagtaaaag aatatcttaa tataacttgg atagaagaag atgcaaaaat
    25741 agaaaaaatg gttgtaagag ggattaatta cttagaaaat gaaatagccg gaatagcatt
    25801 agattttaaa gataatttta atagagagtt gttatttaac tattgcagat atgtaagaaa
    25861 caatgctatt gaatatttcg aagaaaattt tagtaaagag ctttttagac ttcaaattaa
    25921 aagcgcagct aatgaggtaa caaagatatg aaaaaaataa aatttgataa tttcaatgat
    25981 ggtattattg aatttggaga atataaagaa agctacgata tagaaggtaa tgcactagat
    26041 gaaaaagagt ttattgtaaa tagtaaactc ttttattcta atgaatatat aagagagcag
    26101 gacagactta aatttgataa tacagggtta aaagtatcta ttaaattaaa agttccttat
    26161 atgaaattaa ttaaaacaag tgatgttata aaactaaata atgaatttta cagcatagta
    26221 aagctagata ttagtaatga caaaactagc atatatttat atttatctga attagttgac
    26281 gtactagata agcatatttc tatttttatt aaagaaagaa aaaatgttct tgaagatgta
    26341 gaaatgaaat tttataaaaa ggtttgggct tgtgttgaag atttaaacag caaagaattg
    26401 atagaaaata attcaattaa aacttctaca aataaaaagt ttaaaattag atatattgaa
    26461 gaattagatt taagtataaa acaaaatgct ataactaatt ttgttattaa atacaaagat
    26521 aaattttaca atataactca aatcttaaat ttagaagaag aaaataaaat acttgaaata
    26581 acagcagagt ctaagtaaaa aaataggtgg tagtatgcat tcaaaaatag ttactatact
    26641 tgaaaaatta aatataggaa ttggctttca agaatatgac aatttttcaa atgatgaaga
    26701 atatataata ttttttatag aaaatgaata tgatgcaaat ttttctgata atgaaaataa
    26761 aagtattgta tattcaataa agatagagta ttggtataag agcttaaaga atataaacaa
    26821 atatattagt ataaaagata tatttaaaaa agaaaatttt atctttttaa attctagtga
    26881 ttttatagaa aatgaatatt attgtaaaga attaaaattc aaatatgagg aagttattta
    26941 aatgagtgta gagataacaa cagaaggttt tgacgctgtt ttatctaaaa tagagagcat
    27001 gggcaaatcg ggcgataaac ttctaaatga agctgtaaaa gctggcggca atgttatttt
    27061 acaagatgca ttaccaaggg tcagtaaaag aagcggaaag ttaaaagatg gtttaaaagt
    27121 aagtggagtt aagaaaaaag gtggtactaa gtatgtttta gttggtatta cgaaagagga
    27181 taattcaaaa attttttatg gaaagttttt agaatttgga gcatctgcgc ataaaatacc
    27241 aattaaaaaa ggtaagaaaa aaggaagaat aataaaccat ccaggtgtta gtcctaaacc
    27301 gtttttagca cccgcatacg agtcaaaaaa agatgaagct aaaaatgtaa tgaaagaaat
    27361 attaaaaaga ggattagggt tataatgatt aatataaatg atttagtaat agaaacatta
    27421 aaacctttaa aaattcctgt ttcatttcaa aggtttgatg gagaagaaaa aacatatata
    27481 acttttttta gttatttaga acaaaaccgc tatgctgatg atgaagtagt aggaacagaa
    27541 cattatattc aaatagattt gtttagtaaa aataaaatga gttatataaa taaagaaatt
    27601 gaaagacttc ttaaaaaaaa taattttata aaaagaagta tacacgaaat aaaagaatct
    27661 gattatagtt accatactat ttttagattc ttattttttg cagaaaaaaa ggaggattaa
    27721 gaaatggcta ttataggatt aagaaactat agacttgtta aattgcttgt tgatacagaa
    27781 agcgaacttg aatatgacac taaaataatg aaattaacag gtgctaagaa tataaaaata
    27841 gcaccaaagg ttgatagtgc ggaaaattat ggtgatgacc aacttttaga aactgcttca
    27901 gcaatgggag ctatagaagt agaatttgag gttgctagtt taacattaga agaaaaagct
    27961 tttattttag gttacaaata caaagatggc gttttggttg aagataaaga ttttaatccg
    28021 ccaaacattg cgcttggctt tgaatctcct aaatcgcaag gtggaaatag aatggtttgg
    28081 ttaactaaag gcgtatgtga accacttgaa gaagaagcaa aaacaaaaga agataaagtt
    28141 gaatttcaat ctcaaaaaat taagcttaag tttatgccac gtataaatga tggtagacat
    28201 aaaataacag cagatacaga tgttgaaaac tcaccaactg cggaagaatt ttttgcaaca
    28261 gcttttttaa aaacagcagc aaagccaaca gcaccagcaa aaacaaattt aaataaataa
    28321 ggagaaaatt aaaaatggaa ataaaattaa ctataaatga agaagaaaaa atattcaaag
    28381 cacctcctat tgcacttaga aaattagata aagtttttac actaaccgat aaaatcgaag
    28441 atggtcttaa ctctacagaa ttattccgcg aattgcttga ttttacagtt gatatatacg
    28501 gggaacaatt ttcaaaagat gagttattag atggttttta tccagctggt gattttataa
    28561 ataaagcgtt agaagattta tcaaaagtaa gtggaggttt cgaagaaaaa gtaaaaaact
    28621 aggtaatgga gaagatacag aaaataattc taataaagaa aataggtatc tatctccacg
    28681 tgattttata aaagatttat atagcatgtt tttatttgaa aaaaattgga gcatgacgga
    28741 catagacaac atggatatat tttattattt agatatactt gtgtatatga aagagaataa
    28801 aaaagataat aataatccaa atgaaaatga tgtttatatt gaccagataa gctggttata
    28861 aggatgtgag tgtatgtgta agtactgtga agttaaaatt aatacagaac cgtttagcat
    28921 gtatgataca gttgtaagag gtgaattatt aactaacgac tccgacatag atatgtatat
    28981 agaacttaaa aacggaaagc attttctaac aggagcaagt tacgactatt taggaatcag
    29041 tagtaaggaa actattaaat attgtccatt ttgcggaaga aaattaattt aatataagtt
    29101 ttatataaaa taaaccaaga taaaaatctt ggtttatttt tttgcaagtg aggtgagaga
    29161 atggcggaag atgtaggtga attagttgtt cgggtcgcta tggaaaatag ttcatttcag
    29221 caaggtgtta ataatctaaa taggcaaatg aagttaataa gaagcgagtt taaaaattct
    29281 ggcgctggag tgaaaaattt tggaacaagt cttgatggtt tgaaatctaa gcaacaaatg
    29341 ttatctagta ctatacagca gcaaactaaa gtagttgaag catataaaaa taaactaaat
    29401 gaaagtaaaa caactttaag caacacagca caaaagcaag ttgaacttaa agaaaaagtt
    29461 aatcaagcta agagtgctta cgaacagagc aaagcgacgt taggtgaaaa tgcagaagaa
    29521 acaaaaagat taaaatctga acttgatagt ttagaacaag aatatgctaa aaatgaagaa
    29581 aaaataaggt ctaactcggc agcggttgat aattggtcta ttcgagttaa taatgcagag
    29641 tcgagacttt ctgaaatgcg ttcagaacta gatgacacaa cagaaagaat aaatcaacag
    29701 gaaaataaat ggaatcagtt ggcaactaaa atgactgaaa taggtaataa attcagcttg
    29761 gttggaaaac aaatgcaaga tgtaggtaaa aaaatgacta tgagtattac tgctcctatt
    29821 ctagcagtcg gagcagcggc tagtaaactt ggaatggatt ttgaagcatc tatgtcaaat
    29881 gtacaaggat tgagtggggc aacagctgac gaaatggttc aacttgaaaa agcagctaga
    29941 gaagcagggg cttctacatc aaaaacagct aaagacgcag cggatgcgct tggatatatg
    30001 gctttggctg gatgggatgc aaaaacatca atggaggctt taatgcctgt tttgcgattg
    30061 tcggaagctg gaaaccttga tttggcaaga acctcggatt tagtaaccga ctctatgtca
    30121 tcacttggac ttagtgtaaa agagttacca acttacttag accaagttgc taaaactgcc
    30181 gcaagttcaa acactaacat tgacgcactt atggaagcta tgattgtgtc tggaggtact
    30241 tttaaaaatt taaacgtacc tctttcggaa gctaatgcac tgcttggaat acttgcaaac
    30301 agaggactaa aaggcagcga ggcaggaaac tctctaaatt caattatgat aaatttgaca
    30361 agtggagcgg gtcaagctgg tgtagctatg aaagaactag gattaagtgc ttttgattcc
    30421 aatggtaaat tcaaaggtat ggctaatgtt ttgatggaac ttaaagaaaa aacaaaagac
    30481 atgacagaag aacaacgaac aatgtatcta tccatgatag gcggaaaaac acaaatatct
    30541 actttgcaag ccttgctatc tggagttgga gaagagtatt cagaactcga aaaaaaagtt
    30601 ggaaatagta caggcgcatt agataaaatg gctaagacta tgcaaaataa taataaaggt
    30661 tctataacgg ctttaatatc agctgtacag gaactaggac ttaaaatata taatgtacta
    30721 aaaccttcta tagcgcaagc ggttagctgg tttcaaaatc ttacaaataa attaaatgaa
    30781 atgaagccta gcacggtaga aactatagtt aaaatagcag gattagcagc agcgctcgga
    30841 cctgttcttt taattggtgg aaaaatagtt agtatgattg gcggagctat aggggctttt
    30901 ggtacttttt cagcagcaat ggcagtcgta acgacaggag cagccgcagc aactccagct
    30961 gttggtgctt tagctgcaat tttaggtgca ttatcaagcc ccgcagctgt agctatagct
    31021 ggaattactg cgctaggcat atcggttgca acagtcgcta aagacatgtc aaaggatgct
    31081 atagagccaa ttagccgttt tggtaaaagt gtatcagaat ctacaaaaga agctgtaggc
    31141 gcttttatgg atttagaaga aaaaacgacg atatctttaa atcaattaat gtggagtggt
    31201 gagactgttt cagagaaaat gaaacaatca atagtatcta attttacaga aatgtcaaat
    31261 caaatcattg ctaaacttca agagagtaaa gagcaaggta ttcagtcttt gcaagaaatg
    31321 tttgcaactt ctaaaaattt aagtgataaa gaaaaagaag agttaataaa aaatactgaa
    31381 gaagcgtatt caagcaaaga agaaaagata aaaaagagca atgaaaaagt aaatcagata
    31441 atgactaaag ctagtcaaga acacagaaca ttaacaaaag aagaatcagc agagataaat
    31501 caaataaaaa cagatatgct aaataccgca gttgttacaa tgagtaaaag cgaagcagag
    31561 caagctgcaa taatggaaag aatgaaagca aatcatgttg atttgtcagc taaagaagct
    31621 gcggaagtag ttaaaaactc aataaagaaa aaagacgaaa gtataaaagc agctaatgaa
    31681 gaatataacg aaagattaaa aatagccgca cagcttagag cagagggtgg agcagaaaat
    31741 gaaaagttag ctgataaagt agtagaagaa gcaacaaggc aaaaagacga atctataaat
    31801 aaagctaaag aaatgcacca gggagtcgtt gcagaggcgc agaaacaagc agaaaaccaa
    31861 gcaagtaaag tcgattggac aactggagaa ataaaatcaa aatggcaagt attaaacgat
    31921 acagtaaaaa gcaaaatgca agaatgggac agtatagctg gcgactgggt agaaggcttg
    31981 aaagaaaaaa tatctactgg atgggctaat ttgaaggaaa atacagctat tttatgggat
    32041 aacataaagc ttactgtaac aactaaatgg acagagataa aagagtctat aagtttaaaa
    32101 atagaagaaa taaaacaaag tattagcgaa aaatgggaat ctataaaatt gttctttaca
    32161 acaacatggg aaagtataaa acttattttt agtaatgctt gggatgctat aaaacaacaa
    32221 ttagaagaga aattcgcacc tttcatcgaa agattaaaaa tcatatttga tggtataaaa
    32281 gagtttttta gaagtacttg ggaagttata aaaaatatag tacttggtgc tgtgttatta
    32341 attttggatt tagtaacagg gaattttgat aagttaaaaa cagatgcaat acaaatttgg
    32401 gaaaacataa aacaaagctt atcggatgca tgggaatcta taaaagaagt gtttaacaat
    32461 gcattagccg caataggtga tttactttct aatatgtggg aaagtatgaa aagtactgca
    32521 ataactattt gggagtctat aacgcaaact ctaagcagca tatgggaaaa tataaagtcg
    32581 acatgttcga ctgcttggga agggctaaaa acaatttgct ctaatacttg ggaaaatata
    32641 aagtcaactc ttgcaaatgc gtgggaaagc atgaaaacag ctgtaacaac taagttagga
    32701 gaaattaaaa ataatattgt gacaacttgg aataatgtca ttacattttt taaaacactt
    32761 ccagctaaat taaaacaagc agctatcgac atgtttacaa gactaagaga aggtatagac
    32821 tcaaaaaaac aagatgctgt taatgcgtca caaaaaattg gaactgatgt tgtaaataaa
    32881 ataaaagaat ttccaagtaa attactaaca gctggtcaag aattagtaaa cggtttagta
    32941 aaaggaatat caggctttgc ttataaagta gtagacgaag ctaaaaagtt agggcaaaag
    33001 gctgctgatg cagttagaaa tgcgcttgga atacattctc catcacgtgt catgattgaa
    33061 attggtaaat ttgttgatga aggtttagca caaggtattg ctaataatag cgactcggtc
    33121 aaaaaagcaa ctgaaaagat aacaaagatt atagaagaag aaacaaagaa agctacaaaa
    33181 gcatgtgaag cagatataaa actatttaat aaacaaatag acgaattatc taaacaagaa
    33241 aaagcagcac ttaaaaaagt aaaaggtaat gctaaagacg cattgaaaga agaatacgca
    33301 gctaaaaaag aaaaaataaa agaagctata gaactaagaa aagaacaaaa agaaaaagaa
    33361 gttgaacagt taaaagagat agcaaattca gctaaagagc agttgaataa agaattgcaa
    33421 gacagacaag aatttgtaaa aaaagttgat gatttaacaa aagcgatagt tgatgcacta
    33481 aaagaaaaat ataaacaaga atatgaagca aaacaagcaa gtataaaaaa agagttagaa
    33541 gcattagata gatggaagga agaaagtata gatagaatta atagtgtcta tgatgcgaaa
    33601 attaaagcta ttgacaacga attagaagct tttgaaaaag ctgaaaagga aaaagataga
    33661 cttgaacaag acaaagaaga attaaacaaa atagaagaaa ttaaaacagc tataaagttt
    33721 gaacatgatg acactaacaa agagcagttg caaaaagaat tagaaaagat tttagctgaa
    33781 agaaagaaaa gaatagagca gcaagaaata gaagataaaa aagaagcttt aaaaaagaga
    33841 aaagaagaat tggaagaaaa gaaaaaagac gaaatagaaa atataaatca aatctatgaa
    33901 agtgaaaaag aatattacaa taaaaggctt gaagatgcta aaaagttcta tgatgaaaga
    33961 ataaaagagt ctaaattaca agcagaagca gaaaaaatga taatggataa aaatcaaaaa
    34021 gaaatagttg cgttattaaa tagttattca gatgcatata aaaacgcagg tcaaacctta
    34081 ggtgaaaaat tacttgaagg atttaagcct gctataaatg aaatcaaaaa tctaattgat
    34141 agtattacaa gagaaataaa taatgctaga aatgaagcat tgagtttaaa tagcgatatt
    34201 agaaaaggaa attcagcagc taattcaaat tcaggaagaa gagaagcaac ttataataat
    34261 aattcaagaa cagcgaatta taatgtaaat gttaatagta cagactcaaa tacagcaaga
    34321 gaaatcgaaa gaacaataag aagtctagca tttagcttgt aaaaaaaagg ggtgtgaaac
    34381 ttgcaaaaat taatatgtag aaactcgaaa gggcaagaga ttaaactagg aaactcacgc
    34441 ccttttttgt tagaaaaaat agaaaacgga gcaaatacta gcgctaatat ttcagtttcg
    34501 aacaatctag gtgatggtgt aagtatagat gatataagta taaaagagag attgcttcct
    34561 gtttcagcgg ctatttatgc aaaaaataaa gaagatttag agttaaaacg tgcatattta
    34621 acctctttgt ttaatcctag gctcggagaa atggagttta tatatacaaa taacgcatta
    34681 actagacgcg tcaaaggagt tgtgcaagac ataacatttc aaacaccagt tggattaatg
    34741 caaaagtttc ttatacagct cttaacacca agtccgtttt ggatggatga gtttgtttta
    34801 aaagaagaag ttgctttgtg ggttggtgac ttcgaattta ctttagagat aagtaatgaa
    34861 ggaatagaaa tggggcatag agtaagtaat ttaatttgca atgtaataaa taatggcgat
    34921 attgagtgcg gtatgaaaat acagtttaaa gcacttgcaa cagttgataa tcctagtcta
    34981 tttaatgtaa atacaagaga atatattaag ataaacagaa cccttgaagc tggtgactta
    35041 ttagaaataa caacagaatt tgcaaataag agaatagagt tagtaaaatc aaactatgaa
    35101 agaataaatg ttttcaattg gattgacttg gatagcgaat ttttacaact tgaagttgga
    35161 gataatttat ttagatatga cgcagatgtt ggaattgata atttagaaat gtctatatat
    35221 tataatccgc tttatttggg ggtgtaatat gcaacaaagt ataagaatat tagataaaaa
    35281 tataaattta atcgctgaaa tagataatta cgaagaatta caaattatta aaagatttca
    35341 caaagtagga gagtttagtt taaaaataaa tgctaataaa aatcacgtag ataaattagt
    35401 taaaaataat ataattctac ttggtaaaaa ttataataaa gtttgtttaa taatgcacag
    35461 agaatttatg tatacagaaa caggagaaaa aagcgacgtt ctatttgtca aaggtgtaga
    35521 tttaaaaggt ttattaaata gaaggctcat tgtaccaaag actggagaag ctttcgaaag
    35581 tcacgaaggg acacaagaag agattataaa agcttttgtt aataataatt gtgtaacccc
    35641 ttcaagctca aaacgtgtaa ttgataatct tatcatttca gaaaataaaa atcttggaag
    35701 tgctgatatg tggcgcagct cttatgaaaa tttaagcgat aaaattcaag aaataacaga
    35761 attttgtggg cttggttggg agataacatt agatgctaaa caaaagaaat ttatttttga
    35821 cgtaatcgcg ggtaaagatt tgactattaa tcaaacagaa aatccaccga ttatttttcg
    35881 aagtgatttc gataacataa gagctagaca ctttacagag agtataatta attctaaaaa
    35941 tgttgtgtat gctggagcaa gagaggatgc aacaaagcta gtaatttcag ttggtgaggt
    36001 tgatggtttt gagagaaacg aagtctttgc agatgtaagc gaaaatacta taagtatact
    36061 acaaaaagaa ggcgaaatta aattaaaaga atatgaagag ttaaaaagtt ttgaattaga
    36121 aatagaccct aaaaatactt ttatatatga aaaagattac aatctaggtg atattgttac
    36181 aattcaagat agaaaattga aagtaacaat ggatactaga attgtagaag tacaagaaag
    36241 ctacagtaaa aatggcatga aattaaaaat tacgtttggg agcagtatac caaccttatt
    36301 ttcgaagata aaaaggatgg tgagatgatg gaaaaaagtt ttgtatttaa tagcataaat
    36361 ggcgatagga gatacaaggc agaggacttt agagaatact ttgcaagctt cataggaaat
    36421 ggtgtattcc ctaatccaag tacaaatttg caagtcgttg ataataataa tatgactata
    36481 acagtaaaaa aaggtaaggg ctggattaac ggagctatct atataaacac agacgattta
    36541 gtaattaata tagacgcagc ggacgggatt ttaaatagaa tagacagagt agtcttaaga
    36601 tttgatactt taaatagaaa tattaaatta gctgttaaaa aaggtacttt taatagttcc
    36661 ccagctgcaa cagaattaca acgtgatgca gatgcgtacg aacttggatt agctgatatt
    36721 tacatcaaag ctggagcaat aagtattaca caaagttcta taacagattt aagattagat
    36781 aaaaatctgt gcggaattgt aaaaggtaca gtagaagaaa tagacacaac aacattactt
    36841 gcgcaattga acgcttggaa ggatgaagaa ataagtaatt tcaatgcttg gcgggaaaat
    36901 caagagaata aacaagaaga ttggtataac actacaacaa caaactttgt atcagaattt
    36961 aacacatggt ttgaaagtat aaaaggcatt ttggatgagg atgcagctgg aaacttatta
    37021 aatgaaataa acaaagtaaa agaagatttg gcaagtattg aaataacagc acagcgagct
    37081 actgtagaag atgcagaaaa tttatttgaa gctgattcgc aaggaaataa aaatgttgaa
    37141 atagcattaa accaattagc gaaacattat aactctttag cagagcaaca aggtaattta
    37201 gagatagaag taaatggaca aagagcgaaa ggaattacta tagtaaacaa cttaatagat
    37261 atgatttaat aaaggagcgt gaaagatgac aacgaaatta gaagaaaatg caaaactaag
    37321 agaaatgata gatgcattag aaactgcaaa aaatgacttg caagtaggta aaaataatat
    37381 agctaatatt ctgggcttac cttttacagg aaatgacaag ttagaaacaa ctaagaatac
    37441 attaaattca attagaagta cttttgtttc aaatttaaat aaaaaaggtg tgtcaacatt
    37501 tgctaataca ccttttaaaa ttttagctga aaatgtaggg aaggtagaac aaggcaacat
    37561 gattgttcca ccttggtatg ttcctaaaaa tgtatttatt gaagctttga gtgcttacgc
    37621 taaaaatgga atagcatgtg tttcagcaga aaaaaaaatc tactttataa acggttatgc
    37681 agctacgtcg gattcaaata acctttgcta tgatgctatt ttaaacacaa caacaactaa
    37741 aaatacacct agttcttttt cgaagtctgc attaattttt tataatggat atatctataa
    37801 attaggtggt tttagtggct attcagctcc tacaactata tgtcaaaaat ataatatttt
    37861 aactaattca tggactgaaa tagcaaatat gcctaccgca agaggaagtg cttgttgtga
    37921 aatgtattca aatcaaatac atgttttgta tggcggagaa gattcttctt catctcaagc
    37981 agttagagtt gttgagtatt ataatacaac gttaaatact tggtctacaa aatcaactat
    38041 aatagattca aaacaatcgc attattttgt ttgtgctaaa gtcagcgttt ataaaattgt
    38101 tgtgtttggg ctaagaagtt ctatttctgc tcactatctt acaacaatca attatgaccc
    38161 tgtaagcgga actagcagta aggcaggaga aggctttgat agtaatactt ttgccgtacc
    38221 actaggttca actttgtatg tgtcagatta tggttataaa gctggaagtg gcaaatgtgg
    38281 agttgttatt ttttctgata gtggaattaa aaaattaact tttgataaag gaactgttcc
    38341 cagttatagc tattttggag cttcggttga tggtagacaa atatatttta ttttaaatgg
    38401 taagattact tgttttatac cagaattata aaaaaaggag agaatgaaaa tgataaaaat
    38461 atgggataaa aaagaaaaaa taaatggagt agaagcagaa gaaattttaa aaagtaatta
    38521 tgattttcaa actagcgaag tgtttttaat acttgatgat tatgaaagag ttacaaatat
    38581 agagtcagtt aatacaatta aatctatata taaactagat aagagtttga cagctttaga
    38641 aacagcagaa aaatatttag aaatgcataa gaaacaagaa gaagagataa aagcacatga
    38701 ggaactagaa aaaaattcta aaaatgcaac tatagctaca cacgaagaag taaaagcatt
    38761 aagagaagaa acagcagcat taacttttgc aatgattgaa aaggagttga tatagatgtg
    38821 gtacaaaata ataaaaaaat actatgaaga tgaattgtgg tctaaagagc aagttaaaac
    38881 agcagtagaa aagaataaga taacatcaac agaatataaa gaaattgtag gagaggatta
    38941 tattgtatag tcctttttta atttt
//
LOCUS       DAEMHZ010000020        38879 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000020 DAEMHZ010000000
VERSION     DAEMHZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.020"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..280
                     /locus_tag="KPK72_000960"
     CDS             <1..280
                     /locus_tag="KPK72_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2488201.1"
                     /translation="KLSRSISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNK
                     CGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            399..917
                     /locus_tag="KPK72_000961"
                     /pseudo
     CDS             399..917
                     /locus_tag="KPK72_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            944..2311
                     /gene="rlmD"
                     /locus_tag="KPK72_000962"
     CDS             944..2311
                     /gene="rlmD"
                     /locus_tag="KPK72_000962"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2488202.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRIIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHVETCVLL
                     QRQTM"
     gene            2555..5608
                     /locus_tag="KPK72_000963"
     CDS             2555..5608
                     /locus_tag="KPK72_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2488203.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFRNGYKTRADASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     IYPLTIKAGETATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNENAVLVEGKLYKKNEVVKTIR
                     TDIEGIASTSADLLPYGKFRIVESEAPNGYLTDGAKPIDFAITENGTIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSSDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     HLVIDLGTLTDEYEKEISIHTTATSKDGEKSILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEVPDEPEKPETPQTPE
                     KPSRPSDSPKTGDTTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     gene            5847..6173
                     /locus_tag="KPK72_000964"
     CDS             5847..6173
                     /locus_tag="KPK72_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2488204.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            6189..6572
                     /locus_tag="KPK72_000965"
     CDS             6189..6572
                     /locus_tag="KPK72_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2488205.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLKEFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNHAPQGQPKK"
     gene            6708..7103
                     /locus_tag="KPK72_000966"
     CDS             6708..7103
                     /locus_tag="KPK72_000966"
                     /inference="COORDINATES: protein motif:HMM:NF025919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HBG2488206.1"
                     /translation="MRLKEMMDEICSRWSLPKDYIEFLFNHENNLYVNVDDDDDDEDL
                     SYEIEIYGAKGLLVGQYGYSYNPVQKAVIEDWNPNYVVIANCNADPYCIDISMDNSPV
                     YYAVHGEGEWEFEKDSESLEEFFEFLGIR"
     gene            7166..7561
                     /locus_tag="KPK72_000967"
     CDS             7166..7561
                     /locus_tag="KPK72_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488207.1"
                     /translation="MLKEQDIKQYLNEKDSTFKENISIIGVIFPSKFTYTHGPVATAL
                     SMQYYALNFSDSGIAIIGLNNVTGKLENEAFLFVPKEEVTSVKFNKKLMSYELEISTS
                     KGTLAFKVNKTMVGASWHKENLATILKSL"
     gene            7650..7880
                     /locus_tag="KPK72_000968"
     CDS             7650..7880
                     /locus_tag="KPK72_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488208.1"
                     /translation="MQKIWNKGHRIRASDKHLVYHFSIRTLLFVFVAVLLLLNMKQLM
                     RTEWEHFSLFENGLTISPYNFICKGRGYCVEE"
     gene            8109..8516
                     /locus_tag="KPK72_000969"
     CDS             8109..8516
                     /locus_tag="KPK72_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488209.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     ISVIELSNLYSISLDVLLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLITL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            8532..8858
                     /locus_tag="KPK72_000970"
     CDS             8532..8858
                     /locus_tag="KPK72_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488210.1"
                     /translation="MDLNIVLAVICGAVALVGAFCVVFQIYHMTVIDATARGLKHPKF
                     WGVFTMSGNNSSGLLMYLIGRRKYPIVNMSESNSKELEKRKKSAGVGLLFLAIGVIGI
                     ICATLI"
     gene            8959..10350
                     /locus_tag="KPK72_000971"
     CDS             8959..10350
                     /locus_tag="KPK72_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2488211.1"
                     /translation="MRKIFNKGHRIRASDKHLVYHFSIGTLLFVFVVVLLLLNMKQLM
                     RTDWEYFSLLENDLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDRGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYSLLYDMIANRITIDEVRAENGSLKLMNNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDMGTSVISEFYTPLVPKGHDFLQTIGRLAQERQ
                     VMPEQQGKEDVIE"
     gene            10445..10834
                     /locus_tag="KPK72_000972"
     CDS             10445..10834
                     /locus_tag="KPK72_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488212.1"
                     /translation="MLDCFLDYSVNPNLRFHSRIDKIIDDVIREKINIPHKFRMRLES
                     RHCVESRIIYFRFRVLDKACLQCNVFLKGLSEEMKEALSDERIDFAVIYEGIQGSHQD
                     TDTDDKDGFCGSQPQVPFLADGACLVR"
     gene            10984..12180
                     /locus_tag="KPK72_000973"
     CDS             10984..12180
                     /locus_tag="KPK72_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2488213.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLDEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            12193..12327
                     /locus_tag="KPK72_000974"
     CDS             12193..12327
                     /locus_tag="KPK72_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2488214.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADCEMKQLEESEDN
                     "
     gene            12328..12549
                     /locus_tag="KPK72_000975"
     CDS             12328..12549
                     /locus_tag="KPK72_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488215.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            12614..12907
                     /locus_tag="KPK72_000976"
     CDS             12614..12907
                     /locus_tag="KPK72_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488216.1"
                     /translation="MGSGIVELIKDIVKYLSSGLAEYFLECKVSDKRQEYFSNKGGKW
                     AKFCFYMSLIVSIILIIIGIIGLVKKKSISIFFLIAGMGIALFMLVDWGTSKK"
     gene            12999..13625
                     /locus_tag="KPK72_000977"
     CDS             12999..13625
                     /locus_tag="KPK72_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBG2488217.1"
                     /translation="MKCIMLHGLGQSSESWIDTVKVFNDDFEVLCPNLSDWLSGKAPC
                     YDTLYQALETYCEQFEEQLNICGLSLGGILAMQYAIEHPEKVNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFQKTDFINLCKSMTELNFKHELKKISCSVLVVCGDKDK
                     INKRASLKLQQQLENAEIAIIENAGHEVNTDNPTLLGQKLNIFFRQYV"
     gene            13807..14280
                     /locus_tag="KPK72_000978"
     CDS             13807..14280
                     /locus_tag="KPK72_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2488218.1"
                     /translation="MDCIIREIKKEEYGLLNDFLYEAIYIPDGVEPPPKSIINCPELQ
                     EYVFDFGKRKDDKALVAEIQGNVVGAIWVRIMNDYGHIDNDTPSLAMSVFPKYRGQGI
                     GTALLQQLLLVEKSLGYSKISLSVQKNNYAVKMYKKAGFLVVAENSEEYIMTVNL"
     gene            14381..14677
                     /locus_tag="KPK72_000979"
     CDS             14381..14677
                     /locus_tag="KPK72_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488219.1"
                     /translation="MNDIFKDMQAKVGCDYISDLPSYKRKVWQEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLNCR"
     gene            14596..15099
                     /locus_tag="KPK72_000980"
     CDS             14596..15099
                     /locus_tag="KPK72_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2488220.1"
                     /translation="MQETRVLVETRGTTGEETISYWFELPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNNPTFKHLSEDCQEYYFDFEAYAFHLQEHGKFLVTEH
                     GIFELPE"
     gene            15117..15620
                     /locus_tag="KPK72_000981"
     CDS             15117..15620
                     /locus_tag="KPK72_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2488221.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            15729..16127
                     /locus_tag="KPK72_000982"
     CDS             15729..16127
                     /locus_tag="KPK72_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488222.1"
                     /translation="MKKIKSYTDIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPAALTWFMSQKTFDGKKPYSFLKSQITYAIRPKITY
                     AGKAVKLHKQTLNETITAVRSVNDVPDKIY"
     gene            16105..18555
                     /locus_tag="KPK72_000983"
     CDS             16105..18555
                     /locus_tag="KPK72_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2488223.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAERKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKDKEDGATLAKEILTFLTGISTRDGDKF
                     PVLISAISKVSEREHRGLLNVITELRQENTPIANHIANHIASFTNYDFAHLLFSDGTV
                     ENTISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            18555..20756
                     /locus_tag="KPK72_000984"
     CDS             18555..20756
                     /locus_tag="KPK72_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2488224.1"
                     /translation="MKERIKGAFTKKKIFHFLKMTLFVAALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLFMAHRARRMERRMARAVSAGGVSGGVAGAVVGNAVANKRAERKNTASKDNRGNPTS
                     SMGQRAGSKMGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQFRQTGRLEKQEQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASKPSAEKMQEVKRPATAPTPKASEPVKTTVIKERPLSSGASDKKATQSAQIAHR
                     QNVEKVVSQETRQNYTKDRRTKVQQTQTVQKNQQTTEKTRNLVTKKGQKKK"
     gene            20753..21760
                     /locus_tag="KPK72_000985"
     CDS             20753..21760
                     /locus_tag="KPK72_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2488225.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ENFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            21776..22687
                     /locus_tag="KPK72_000986"
     CDS             21776..22687
                     /locus_tag="KPK72_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488226.1"
                     /translation="MFKKNKKQTETLKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TTAVKATYTVKVHVDADGDMVIVQNPTLAPAVEKSDYEPKTPEADASVDADTVNDATS
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGDNVKVKVAVKF
                     LDNQTKATQVSQYELVLYKDSNWKIVG"
     gene            22866..23723
                     /locus_tag="KPK72_000987"
     CDS             22866..23723
                     /locus_tag="KPK72_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2488227.1"
                     /translation="MVNKVENIITVINYIENNLTEKLNLSSIAIGVGYSKYHLHRMFV
                     ETVGLSIHDYVQRRQLTESAKLLVFSDKSILEISLIAGYESQQAFTNIFKQMYKKTPN
                     EYRMNEEFYPLQLRFDLIQTVKQKLSQQEMEENIRFATTTDIPACLELAYLVIDGFPN
                     LNENEYLTELERGILEQRVLILTDNTVAIALMSINYHTGSIDFFGVHPLYRKCGIAKL
                     FLNKVMNELLVDKDISVTTFREGDKADTGYRKAYKELGFAEAELLVEFGYPTQKLVLF
                     SKNGGSTNE"
     gene            23716..25068
                     /locus_tag="KPK72_000988"
     CDS             23716..25068
                     /locus_tag="KPK72_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2488228.1"
                     /translation="MNSNPLAKDFNTGSLLKFAFPTISMMLFMGLYTIVDTIFVSRFV
                     NTNALSAINIVCPVINLIVGLGTMIATGGSAIVARKMGAGEPKRAKQDFTLLILFGFI
                     LGIVIFILGIIFIDEIIYALGADKVLFPYCKDYLTIILLFTPASMLQVLFQNLFVTAG
                     KPTLGLVLSVSAGVMNIVFDYLFMVIFDMGIAGAGLGTGIGYLIPAITGLIVFCKKSG
                     TLSFVKPIIDIKVLGESCINGSSEMVSQLSTAITTFFFNRTMMKLLGVNGVASITIII
                     YTQFLLTTLFIGFSMGVAPVISYNFGANNYYGLKKIYKICISFIFVGSLFIFALSFIN
                     GNSLVRLFTGTNEAVYKIAINGFMIFVFSFLFSGFNIFSSAIFTALSNGKLSALISFL
                     RTFGFIMIGLLIMPQFIGITGVWLAVPVAEILTLCISLYLNFRLWLSWNNQNNGMKKV
                     "
     gene            25079..25303
                     /locus_tag="KPK72_000989"
     CDS             25079..25303
                     /locus_tag="KPK72_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488229.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKI"
     gene            complement(25349..26641)
                     /locus_tag="KPK72_000990"
     CDS             complement(25349..26641)
                     /locus_tag="KPK72_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488230.1"
                     /translation="MLNNLYRKLHILFASSVMLIITLVISFVVANTVHTEKINESTLF
                     QRLTTLLIYQVESASSDMDKALKTYEESYHIFSLITDTRGNTIYQSDFPFPTSADNLL
                     HDVEKQISTQPLSQTESNTTSQGGFLEIRGTHHDTYFVIPATIRTANDTVYHATFLYQ
                     TASLTDILQKTLPIYLLIWFWACIVVIVLTRYLLKKSFAPTERILQSQKDFVATASHE
                     LKSPLAVMISNTDMLLDNVSLNEQAKQAVQTIDFECMRLSRLVKDMLLLASSDAKTWT
                     LHKSTINVDTLLITLYETYEPVCMKQNLELKLHLSEESYPAMLTDKDRLFQILCVFMD
                     NAIQHSKNNSLIEIEVIANEKNIAFSVVDHGQGISDEDKHYIFDRFYSGDKSHTNKSN
                     FGLGLSIAKELTQMLNGTINIHDTAGGGATFTVTFPLK"
     gene            complement(26635..27321)
                     /locus_tag="KPK72_000991"
     CDS             complement(26635..27321)
                     /locus_tag="KPK72_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488231.1"
                     /translation="MKILLLEDDLELCKNIKISLEKENYMVDCCNDGETAMLYALNTD
                     YAYDLAIVDRMLPIIDGLTIIKSMRRKGISIPILIITAMSTLDNRVEGLDGGADDYLV
                     KPFHIKELLARVRALTRRPANIQLSSKLQYGDLTFDKDSRTLSTPVNSILLTSKETEL
                     TFVFMQNPETLFSREQLILKIWGSSSEVETGNVDSYISFLRKRLKELKSSCKITTVYG
                     AGYKLEKETC"
     gene            27484..27981
                     /locus_tag="KPK72_000992"
     CDS             27484..27981
                     /locus_tag="KPK72_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HBG2488232.1"
                     /translation="MKKTNISKFIAVGLCICALTGCGASPDEKPDTSNPIVNSNTNEE
                     NANGSSENKGNDILESANLIGSVLEFTDNGCSVSQAKETDGGASLQIEASGMENKDNT
                     VSVTYTPDCEFVVATLNKQLGSVTNITTGSISDVKKQSNVYLYGEFADTLHFNATKVV
                     VARWE"
     gene            27993..29216
                     /locus_tag="KPK72_000993"
     CDS             27993..29216
                     /locus_tag="KPK72_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488233.1"
                     /translation="MKFYQLGFRYLQRKKGKTILLLIVLILVNSMILGTSMILRTTNE
                     SKQAMQEKTNSKIVAEITSEDSKITENEINKIETLEDVSSINRIGRQEVFPNNFAPVT
                     LNTSTNEENLKIALLSYDDLEKDSPFSEMQYRLASGDYIKHEKKGAIINANLANQNGL
                     KVGDTIELGTENGTKVSVQIIGIFMSAGNVEKDQPSETTAVNRIENQIFIDNSTYKEL
                     FKETEFEKLCVYSQRPEKLDVLETSLQKIFGNDVELSTSDTLYQQMSAPLEQISKVAN
                     LMLILTLITGTVVVSLLLCMWMRTRQKEVAIFMSLGKAKSEIFLQTFLEAGSVFTLSV
                     LGATAIGKLFSGVLQNVITGSESVTENLKVVLQIQDIGMLFLLGGAVILVALCCSILP
                     ILRANPKDILAKMEG"
     gene            29220..30662
                     /locus_tag="KPK72_000994"
     CDS             29220..30662
                     /locus_tag="KPK72_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488234.1"
                     /translation="MNFFGLARRSVFRKPVKSILLLLIVFAISTLLLAGVASKNASIE
                     VQDKTRQAIGAGFLLEKNAEYRTKRISELSEKIGEKDGELEGYHQEKVVVNGAEGWRG
                     WTTNEFETLDIKDIEKLAKTNGIADYNITTAITAVNPVNFQRIEDKDVDQSVDVGGVS
                     LIGNKDMKLDRNVLSGNLHIKEGRMITPKDKDMCVISEELAKQNNLKIGDKISFNDYH
                     DTENSKVSEAEIVGIYQVDQKMSPLMQGDTYRSENVIFTDLRFPEKAEGETSPLYERA
                     YFKVEDVEAYDEVKENLQKVDINWEQYDLIDNNGNSETMSSNFNDLAKVSEMMILVIS
                     VASFVILVFVFLFWLKNRVQEVGIFLSLGVPKLRIIGQIWSEAIMIAVLSLMLSFAVA
                     PAVSKVTANYLVSQQVQQMQEEEKNNEGKVSTEYVAPKQDVQNISVEVTPEMYLLDGV
                     SVLVLITASVLVSGIVILKRNPKDILSEMS"
     gene            30676..31311
                     /locus_tag="KPK72_000995"
     CDS             30676..31311
                     /locus_tag="KPK72_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488235.1"
                     /translation="MLEVKNVSYAYKTKKDKTILNNVSATFEEGIFYTIVGPSGAGKT
                     TLLSLLAGLDTAVKGTVLCNGEDITKKGLNYHRKHNISLVFQNYNLIDYLTTVENVKL
                     GGSGNAEKLLEEVGIGKEYWQRNVLQLSGGQQQRVAIARALASDAHVLLADEPTGNLD
                     ETTADEIIALLKKTAHELGKCVVVVTHSKHLAEEADEILEIKNGAISFLSE"
     gene            31586..32026
                     /locus_tag="KPK72_000996"
     CDS             31586..32026
                     /locus_tag="KPK72_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488236.1"
                     /translation="MKPSELQEKTCHQFDCAIKTVIRHRAIDFYRAIRRSEKKEMPFA
                     EIMDDLLNSIGTEDTYSTDYVAVFEVCGEKVSIEDEQLARALKAIEERKRNIVLQYFF
                     IGDTDTYIGKMLDCSRANVTKCRIQALDKLKQLIEEEKEKCQKS"
     gene            32011..32244
                     /locus_tag="KPK72_000997"
     CDS             32011..32244
                     /locus_tag="KPK72_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488237.1"
                     /translation="MPKELTPTFTVISKACDGDETAISKILEFYDPYINQCCMRPFYD
                     DNNNLRLAVDLEMKGFIKEAIIRKILNFDIKRF"
     gene            32549..32695
                     /locus_tag="KPK72_000998"
     CDS             32549..32695
                     /locus_tag="KPK72_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2488238.1"
                     /translation="MDKKLIRYSMQIAMLNQLLARKMISEKEYALVKSKLMQDYKIVS
                     DITA"
     gene            32768..34522
                     /locus_tag="KPK72_000999"
     CDS             32768..34522
                     /locus_tag="KPK72_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2488239.1"
                     /translation="MKEVEVIKARNDLQTRTRGKTIELMRVGAYCRVSTDSADQLNSY
                     KSQVAYYTDMIKKNKEWVLADIYADEAITGTQVAKREDFQRMINDCMNGEIDMVITKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCLGYDYHKDTKTITVNEEEAEIVRYIFNRYIEGAGGS
                     VIAHELENLGYKTKYGSSTWVQSTVIGIIKNEKYKGDLLLGKTFTVDPISKRRLENFG
                     EEDKFYIRNHHEPIISEEVFEKAQEILARRNVNRGKIGEGNTKREKYSRKYAFSCMLE
                     CGFCGGTLTRRNWHSSSQYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRL
                     LCDDNKDVLEEFLQRMDDTLSSSAVSKQLAKAEKEIDALEKKKSRLVDMRLEEIIDKE
                     TYESKYDDLVSKQEQLIEERHKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVF
                     ESIVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKPQRKNARGRHRTDELCS
                     HDSNEVDKMCSDSSNDTL"
     gene            35072..36106
                     /locus_tag="KPK72_001000"
     CDS             35072..36106
                     /locus_tag="KPK72_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG2488240.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDEY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            36193..36396
                     /locus_tag="KPK72_001001"
     CDS             36193..36396
                     /locus_tag="KPK72_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG2488241.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            36567..37727
                     /locus_tag="KPK72_001002"
     CDS             36567..37727
                     /locus_tag="KPK72_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG2488242.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFVTDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            37986..38126
                     /locus_tag="KPK72_001003"
     CDS             37986..38126
                     /locus_tag="KPK72_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488243.1"
                     /translation="MENLMMSIITGVIDSYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT        13319 a         6112 c         8350 g        11098 t
ORIGIN      
        1 taaactatct cgctcaattt ctgatgtgtc atggtcagaa tttattcgtc aattagaata
       61 caaggctaat tggtatggta gaaaaattgt aaaggtaggt aaattctttg caagttcaca
      121 aatatgtaat aaatgtgggt ataaaaacga ggaagtgaag gacttaaata taagagaatg
      181 gatttgtcct agttgtaatg aaactcacga tagagatata aatgcaagta taaatatatt
      241 aaaagaagga ttaagactaa taacaattca aaataaataa cgtatataag aaccgtagga
      301 actatgggga tagcctgtgg agaattagta agacatattt tagtatgcaa aattctgtga
      361 agcaggaacc ctgtgacttt agtcatggga ggttcaggat gaataaacct gatggatata
      421 tatcagcaac tacagataaa tatgacccaa cagtattaga tttaattgat ttatcatact
      481 tagcttttga accatttcct gttggaaggt tagataaaga cacagaggga ttattagttt
      541 taactaatga tggaaagctt tctcatagag ttttatctcc taagaagcat gtgcctaaga
      601 cttattatgc aaaaattgat ggagtagtta ctgaagaaga tgtagaagct tttttagaag
      661 gtgtagtgct tgatgatgga tataaaacta tgccatcaca gttaaatata ttaaaaagtg
      721 atgatgaatc agaaatagag cttacaatac atgaaggtaa gtttcaccaa gtaaaaagaa
      781 tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag attgtctatg ggcaatttaa
      841 aattggatga aagtttagag ttaggtgagt atagagagtt aacagatgaa gaagtaaaat
      901 tgattgaaga aagataataa agatactata gtaaattgga ggaatgtctg tgaagaaaaa
      961 agatgttata gaatttgaag tagataaaat ggaatttggt ggcacatctt taagccaagt
     1021 tggagataga attatccata tgaagggtgg aataagtggt caaaaggtga gagctggagt
     1081 taaaaaagtt agaagtaaga aagctgaagt taagatgata gaattattag agaactcacc
     1141 acttgaaact gaagaaactt gcaagcactt tagagagtgt ggaggatgta cactattgtc
     1201 tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa gttatggatt tatttttaaa
     1261 gcaagattta tttggatttc agtttttagg gatagaagga agtccagaaa acaaatatta
     1321 tagaaataag atggaatata cttttggaga tgaagtaaaa aatggacctt tgacattagg
     1381 tcttcataaa aaaggaaagc atatagatat acaaactgtt gaggagtgta tgttggttga
     1441 tgaagacttc tcaaagatat tagtttctag tgtagagttt ttcaatgaaa aaaagcttcc
     1501 ttattataga acaatgaatc acaaaggata tttgagacat cttgtagtta gaaaagggat
     1561 tcacactaat gagattatgg ttaatatagt aacttcttct caagaagatt ttgatatgta
     1621 tgagtttaaa gatatgcttt tagggattga tttaaaatct gagttggtgg gggtacttca
     1681 tactattaat gatggtttag cagatgcagt ccaatgtgat gagttaagag tattgtatgg
     1741 aagagattat atacaagaag aaattttagg tcttaagttt aagatttcac cattttcgtt
     1801 tttccaaacc aatactaagg gagctgaggt tctttatagt attgctaggg attttattgg
     1861 agattacaat gataaagttg tatttgactt gtatagtggt actgggtcta ttggacaggt
     1921 tatggctggt gctgctaaga aagtgtatgg tattgagata gttgaggaag ctgttgttgc
     1981 tgctaatgag aatgctaagt tgaatggact tactaattgt gagtttattg ctggggatgt
     2041 ggctaaggtt gttaaggatt taaaggacaa gcctgatttg attattgttg accctcctag
     2101 accaggcatt cataaggatg ctattaggga tatttgtgga tttggtgcta aggagattgt
     2161 ttatatttct tgtaatccta agtctttggt tgttgatttg gttgatttta aggggtatgg
     2221 ttatgagatt aagatggtta agtgtatgga tatgttccca aatacgccac atgtggagac
     2281 ttgtgtgttg ctacaaagac aaactatgta gaaatcctta attttaggca ctttttcaag
     2341 catatcgtct ttacgagcga gggcgataaa atcagaaata aagagataaa aaactatatc
     2401 aatgtttcag tagttgttga tatagagtgt gggaacacaa acaattaaat actaaacttt
     2461 aggggtactt gttaaaaaag tatccctttt caatgtaaga cgttcaaaat agagcgtcta
     2521 tttttttacc aaaaatgaaa ggagcattag atttatgaaa aagatgttaa aacgattgtg
     2581 tacgggcttc ttagctcttg caactgtcgt tactgcttta ccgacgacac ccgttcatgc
     2641 agaaagcaag caatactgga cggagtcaaa agagcgtgtc ggtatcgttg aaaaagtaat
     2701 gaatgacggt tcgattggtt ctacctttaa cgagggacat ttgaccgttg agggcgagga
     2761 cgcttactgt atcgacatta acacagattt taggaacggc tacaagacca gagctgacgc
     2821 aagcacccgt atgagtgccg accagataag cgacgttgcc ttatctatcg aatatgtgaa
     2881 acagtacaca gacagccaca gcggaataag caaaaatcac gcttaccttt taagacagct
     2941 tgtagtctgg cagagattaa gcgtacatct tggctggcaa tgcgataacg tgcgagcttc
     3001 ttatgatgaa attccaaagg caacgcagga cgaagttttc tctggtgcaa gagcctttgt
     3061 caaagaaaat aaaggacgtt atgaatgtgg cggttatatc tactctggcg aggggcagga
     3121 attggggcaa ttctgggcga aattaaatgt cggaaatgcg aaacttaaaa agacttccag
     3181 taacaccagc attacagacg gtaacggaaa ttactctatc gctggtgcaa catacggtgt
     3241 cttttctgat aaggactgca cgaaacagct tgccaccctt acgactgata aaaacggaaa
     3301 cacagatgtt gcagaggtaa aggcaggcac agtctatatc aaggaacttt ccgcaccagc
     3361 aggatataaa gtggataaaa caatctatcc attaacaatc aaagctggcg aaacagcgac
     3421 cttgaaagta tcagatacac caaaagtaac agatacgttg attgagcttt tcaagattga
     3481 tatggaaaca cagaaagaca atccgcaagg gaacgcttct ttagcaggtg cggaatttac
     3541 atggaagtat tatgctggct tctacaataa agacaatctc cctgccgaag ctactcgtac
     3601 atgggttaca aagacaatcg ctgaaacaga cagcaatggg acaacccact acatcacaaa
     3661 attagcggac gcatacaagg tatctggcga cagcttctat atgcaggacg gcaaggcggt
     3721 tcttccactt gggacactta ctgttgagga aacaaaagct ccaaacggct acttgttaga
     3781 cggtgcatat atgcaggctg gcgataagtc cgaacagatt aagggcttat atgtaacaca
     3841 gattaccgag gacggcgacc ttgccgtatt atctggaagt aaccagtttt ccgtatcaga
     3901 caaggttatc cgtggcggtg tcaaaattca gaaacgagat ttagaaacgg gcgatacaaa
     3961 gccacaagga agtgccactt tgaaagatac agcttttgac atcatttcct taaatgaaaa
     4021 tgcggtattg gttgagggta agctctataa gaaaaatgaa gtggtaaaga caattcgtac
     4081 agatattgag ggtatcgctt ctacttctgc tgacctttta ccttatggaa aattccgtat
     4141 cgttgaaagt gaagctccaa acggatattt gacagacggt gcaaaaccga ttgattttgc
     4201 aatcacagaa aatggaacaa tcgtggactt aaccgacgaa gcccattcta tctataatca
     4261 gattaagcgt ggcgatattg agggtgtaaa aatcggtgca ggcacacaca aacgtcttgc
     4321 tgatgttccc tttaggatca caagcaagac aacgggcgaa aatcatgtgg tggtaactga
     4381 tgataacggg cagttctcca cttcttctga ctgggcttca cataagcaca ataccaacgc
     4441 aggcaagacc agcgaggacg gtgtatggtt tgggacttct gaaccagacg acagcaaagg
     4501 tgcgttacct tacgatactt acatcattga agaattacgc tctgatagta acaaaggctt
     4561 tgaacttatc ccaccttttg aaatcgtggt atcaagaaat catcttgtga ttgatttagg
     4621 gactttgact gatgaatacg aaaaagaaat ctctatccat actacagcga ccagcaagga
     4681 cggcgaaaag agtatccttg caggaaaaga ggtaacaatc gttgatacgg tcaaattaga
     4741 cggacttacc aaaggcacaa agtatcagct caaaggctgg cagatgttaa aagaagaaaa
     4801 tgccgagctt atcattgacg gaaaacgtgt agaaaatgat tatacttttg tcgctgatga
     4861 ggaagaaatg aaagtagaaa tttcctatac attcaatgcg tccgagcttg gcggtaaaaa
     4921 tctggtaaca ttcgaggaat tatacgactt gagcaatcca gacgaacccg tgaaagttgc
     4981 ggaacataag gacattgagg acgacgggca gaccgtttta atcacagagc gtatcatcaa
     5041 aattcatacg actgctacgg ataaggacgg caacaaagag cttgaagctg gaaaagacgt
     5101 tacaatcatt gataccgtaa ccttagaggg cttggaagtc ggtacacagt acaaacttgt
     5161 gggctggcag atgttgaaag aagaaaacgc cgaacttctt atcaatggaa aacgtgtgga
     5221 aaatgattac acttttactg ctgacagcga aactatgaaa gtggaagttg cctttacctt
     5281 tgacgctact tctcttgacg gcaaacagct tgtaactttt gaggaattgt acgacttgag
     5341 caatccagac gagccgaaga aagttaccga gcataaggac attgaggata agggacagac
     5401 gattaccttt aaggaaaagc ccgaagttcc agacgaacca gagaaacccg aaacaccgca
     5461 gacaccagaa aagccgagca gaccgagcga cagtcccaaa acgggcgata ctacaaatgt
     5521 catggcattt cttgtgatgt tacttgcgtc tgctggtgga ctggctggaa catatcttta
     5581 caaacgccgt aaaatgaaga aatcataagg cgttaacggt atgatgaaag aaaaatcacg
     5641 ctctccgccg aagctgtcaa acggcagact tccgcttatt ctggcttgtc tgcctacaac
     5701 ctgcaagaac acggatagtc aagggtgcgt aagcattgat tttacccttt actatctgtg
     5761 ggattgctgg tacttcccaa accgccagaa taagaaatca caatcaaaaa acttatcaaa
     5821 aatagaaaga aacgaggtaa aacaatatgg aaatgaaata tgtcgtgcca gatatggcac
     5881 agtcttttgg aactcttgaa tttgcaggcg aaagcgagcc agtctttgaa agagataaaa
     5941 ataaccgcag ggtcttagcc agacgaagct ataaccttta ttctgatgtg cagaaaggcg
     6001 aaaatgttgt ggtggaaatt cccgtgcagg ctggcgaaaa gaagttcaag tatgaacaga
     6061 aagtaaaact tgtcaatccg aagttatacg gcagaggtta cgcaatcggg gatatgggac
     6121 ataccgatta tgtgttagtc gctgatgata ttgtagcagt tgaagaaaag taaaggagtg
     6181 aagaatttat gagattatca aacgggtttg ttattgacaa agagaaaaca tttggagaat
     6241 taaaatttac agcggtgcgt gatgtgttct tgcagaatga ggacgggaca ccgagtaccc
     6301 agcttaaaaa gcgtatctat gatttaaagt gcagtctgca tggtggaatt atccccgtat
     6361 ctgtaccgcc agaagtaccg ttaaaggaat ttccgtacaa tgcagttgtg gaacttgtca
     6421 atccagtagc cgatacagta tcacgaaaaa cttatacggg tgcagatgtg gactggtatg
     6481 taaaggcaga ggatattgta ttgaagaata aaggcaacca gaacgcaggc agtccacaga
     6541 accatgcacc gcagggacaa ccgaaaaaat aggattgccg aatagatttt cattgtggcg
     6601 gatatatgaa actgttataa tgtagatatg atgaacgtga taaattggaa tttgacatat
     6661 taaataagta atcaaaagtt tgatgatagg aaaggagaat cagaaatatg cggttaaaag
     6721 agatgatgga cgaaatatgt tcgagatgga gtttgcctaa agattatata gagtttcttt
     6781 ttaatcatga aaacaaccta tatgtaaatg tcgatgatga tgatgacgat gaagatttat
     6841 cctatgaaat tgaaatatat ggtgctaaag gtttacttgt tggtcaatat ggctattctt
     6901 ataatcctgt gcagaaggca gtaatagagg attggaaccc taattatgtt gtgattgcaa
     6961 attgtaacgc tgatccatat tgcatagata ttagtatgga taattcccct gtatattatg
     7021 cggttcatgg cgaaggcgag tgggagtttg aaaaggatag tgaatctttg gaagagtttt
     7081 ttgaattttt gggaatacga tagaaaatgg taatacaacc acaaattctg attgaatgct
     7141 gtaattttat aggaggataa taaaaatgct aaaagaacaa gatattaaac aatacctcaa
     7201 tgagaaagat agtacattta aggagaatat atctattatt ggtgttattt ttcctagtaa
     7261 atttacatat actcacggtc cagttgcaac tgctctttca atgcaatatt acgcattgaa
     7321 ttttagcgat agtggtattg ctattatcgg tttaaataat gtaactggaa aactggaaaa
     7381 tgaggcgttt cttttcgtac caaaggaaga agtaacaagt gttaaattca acaaaaagtt
     7441 aatgtcttat gaattagaaa ttagtacatc caaaggaaca ttggctttta aagtgaacaa
     7501 aacaatggtg ggtgcttcat ggcataaaga aaatttagct acaattttaa agagtttata
     7561 agtttttcaa aaattcatat ctatacatat aaagcagtta gtcttaggat tgactgcttt
     7621 tttcgtaccc ggaaaggagt gattgattta tgcaaaagat ttggaataaa ggacaccgta
     7681 tcagagccag cgacaagcac cttgtctatc acttttccat aaggacgctt ctgtttgtat
     7741 tcgtggcggt tcttctgcta ctgaatatga aacagctcat gcgtaccgag tgggagcatt
     7801 tcagcttgtt tgagaatggc ttgaccatct ccccttataa cttcatatgt aaaggcagag
     7861 gatattgtgt tgaagaataa aggcaaccaa aacgcaggta gtccacaaaa ccctacaccg
     7921 cagggacaga cgaaaaaata aatctaggtc atagatttta ctctgaaatt acagtataat
     7981 gagccaaaag agccgtattt aagctatgta aaataatttt gacaaggaaa aaaaggcttt
     8041 gtcgaagata ttggatagac atttgagggt taatatatga caatgacacc gaaaggagca
     8101 atcggaatat ggaaataggt aagaaactta aaaatgcacg agtacagtct ggaatgacac
     8161 aagaaaatgt tgcagaaaaa atcaatgtat caaggcagac aatttcaaac tgggaaaatg
     8221 aaaaatctta cccagacatt atcagtgtaa tagagttgag caacctatat tctataagtc
     8281 ttgacgtatt gctaaagggt gatgaaaaaa tgattgaaca tttagaagaa agtacaaatg
     8341 ttgtaaaaag caaccaaaaa atgatatggg caattattgt aaatatcatt gtcgtagcat
     8401 tgctgataac attaaatatg tttataccag ataaccgtta tttcttagtt ggaatatttt
     8461 gtcttatggt tatcacttca tcagcattac tttaccagat aatcaaaaaa ttttaaggag
     8521 gatattttgt tatggatttg aacatagttt tagcggtaat ttgtggagca gttgcacttg
     8581 taggagcgtt ttgtgttgtg tttcagattt atcatatgac tgttatagac gcaacagcca
     8641 gaggattaaa gcaccctaaa ttttggggag tatttacaat gagtgggaat aattcaagtg
     8701 gtttgcttat gtatctaatc ggaagaagaa aataccctat tgtaaatatg agtgaaagca
     8761 actcaaaaga attagaaaaa cgtaaaaaat cagcaggtgt tggactgtta tttttggcta
     8821 ttggtgtaat tggcattata tgtgcgacct taatataact aaaaatgcag atttatgatt
     8881 atatcttgca acatttacaa agcagttagt ctatacgatt gactgctttt ttcataccca
     8941 gaaaggagcg aatgatttat gcgtaagatt tttaacaaag gacaccgtat cagagccagc
     9001 gacaagcacc ttgtctacca cttttccata gggacgcttc tgtttgtatt cgtggtggtt
     9061 cttttgctac tgaatatgaa acagctcatg cgtaccgatt gggagtattt cagcttgtta
     9121 gaaaatgact tgacgctttc cccttacaac ttcataacta tactgatagc gactggtgtt
     9181 tgtgcattgg tcgcttttct gtattatcgc ttctgttacg acagtttcaa gaagctcctg
     9241 caccgtcaaa agctagcaag aatggtgtta gaaaataagt ggtatgaagc cgataccgta
     9301 caagacagag gtttttttac tgacctgcaa agcagatcaa gggaaaaaat cgtctggttt
     9361 ccaaagattt attatcaaat ggaaaaggga ctgcttcata tccgctgtga aattacgctg
     9421 ggaaaatatc aagaccagct tttacggtta gaggataaat tggaaagtgg cttgtattgt
     9481 gaactgaccg acaagaccct gcatgacggc tatatcgaat atagcctgct ttatgatatg
     9541 atagcgaacc gcattaccat tgatgaagta cgggcagaaa acggcagtct gaaactgatg
     9601 aacaatcttg tctgggaata tgacgcactc cctcacgctc tgattgctgg tggaactggt
     9661 ggcggtaaaa cctatttcct gctgacgctc attgaagcct tactgcatac caacgctgtc
     9721 ctttatatct tagacccgaa gaatgctgac cttgcagact tgggaacagt tatgggaaat
     9781 gtgtatcaca ccaaagaaga aatgattgat tgcgtcaatg ccttttatga gggaatggta
     9841 cagcgaagcg aggaaatgaa gcgacacccg aactataaga cgggcgaaaa ctacgcctat
     9901 ctgggacttc caccctgctt tcttatcttt gatgaatatg tggcgttttt tgaaatgctg
     9961 gggacaaaag aaagcgtagg cttgcttagc cagttaaaga aaatcgttat gttagggcga
    10021 caagcaggtt atttccttat cgttgcctgc cagcgtccag acgcaaagta tttctcggac
    10081 ggtatcagag ataacttcaa tttccgtgtg gggcttgggc gtatcagcga attaggttac
    10141 ggtatgctgt tcggttcaga tgtgaaaaag cagtttttcc agaagcgtat caagggacgt
    10201 ggctattgtg atatgggaac aagcgtgata agcgaatttt acacgccttt agtccccaaa
    10261 ggacacgatt ttttacaaac aatcggcagg cttgcacaag aaagacaagt catgccagaa
    10321 caacaaggaa aggaagatgt gattgaataa ggacacatta aatgtttatc acgcacccac
    10381 agagaaaaac tacctctgtt tgtgcttgaa tacagatatt tttacaacgg attgcaggaa
    10441 atatatgtta gattgttttt tagattattc tgtcaatcca aatctgcgat ttcattcaag
    10501 gatagataaa atcattgatg atgttattcg tgaaaaaatc aacattcctc ataagtttag
    10561 aatgaggttg gaaagtagac attgcgttga aagcaggata atctattttc gatttcgagt
    10621 tttagataag gcttgtctgc aatgtaatgt atttcttaaa ggactgtctg aagaaatgaa
    10681 agaagcactt tcagatgaaa gaatagattt tgcggttatc tatgagggca tacaaggttc
    10741 acatcaggac acagacacgg acgacaagga cgggttttgc ggttctcaac cgcaagttcc
    10801 gttcttggcg gacggggctt gtctcgtccg ctgataaaac ccctcggtat ctaacagagg
    10861 ggtacgtttg caaatagaca atagacaaaa aacataggaa acataaggct tctggcatga
    10921 tttcctagta aaattcagtt gtgaagtcgc cttaaaaaac ttcacacttt acagattggg
    10981 ggtatggttc tgaatgaaga acaatggata aaagaattac gggagaaacg gattgcttac
    11041 ggtatctcac aaggcaggct ggcggtggct tctggtatca caagggaata tctcaacaag
    11101 atagaaagcg gaaaaatgaa gccgtcaaag gaacttctgg aaactctgca taaggaactg
    11161 gcaaggttca atccagaagc accgcttacc atgctgtttg attatgtgaa aattcgtttt
    11221 cccacgctgg atatacagca catcatcaaa gatatattaa aattgaatat caattatatg
    11281 ctccatgaag attacggaca ttacagttat acggagcatt attctttagg ggacatcttt
    11341 atctatacgt cggctgacga agaaaaaggt gtccttttag agttaaaggg gcgtggttgc
    11401 cgacagtttg aaagttacct gctggcacag caaagaagct ggtatgactt tctcatggac
    11461 gcactcgtag acggtggcgt gatgaagcgt atcgaccttg ctatcaacga ccatacgggc
    11521 attttggata ttccagagct tgcggaaaaa tgtaggaaac gggaatatat cggaaagtcc
    11581 agaagttata agttttacca gtctggcgaa cttatcaagc acagagagga cgacagagaa
    11641 tatatgggac gtacccttta tcttggttcg ctgaaatcag atgtgtattt ctgtatctat
    11701 gaaaaagact atgagcagta cgtcaagctg ggtacaccgc ttgatgaagc cgacattatc
    11761 aaccgttttg agatacggct tcgtaatgaa cgtgcttatt atgcagtacg agatttactg
    11821 acctattatg acgcagagca gaccgccttt tctatcatca accagtatgt gcggtttgtt
    11881 gatgaagaac cagacaagcg aaaaaacgac tggaaactca atgaccgctg ggcgtggttt
    11941 atcggcgata acagacagag cttgaagctg acgacaaagc cagaacctta taccttagac
    12001 cgtacattgc gttgggtaca aaggcaggta gcaccgacct tgaaaatgct gaaaaagatt
    12061 gataaaggaa acggtacaga ctacatggaa actatcgaac agcaggcaaa gctcacagaa
    12121 aagcatgaaa tgataatcaa acagcagacg acccctgcaa aagatttggt ggaaagttag
    12181 gagtgataaa aaatgtgggt attattaaaa gacttcctgc tggtatctat gggaatgggt
    12241 atcggcgtag tcttgatgtg tattttgaat gtcggcaaag aagctgactg tgaaatgaaa
    12301 caattagaag aaagtgagga caattaaatg aatttcggac aaaatttata tcaatggttt
    12361 ttatcaaacg cacagagctt agtgcttatg gcgattgtgg ttatcggtat ctacttaggt
    12421 ttcaagcgtg agttttccaa acttatcggc tttttggtag ttgccttgat tgctgtcggc
    12481 ttggtattca acgctggcgg tgtgaaagat gtactgttag aactgttcaa taagattatc
    12541 ggtgcataaa ttattgtaaa gcatatatag aagtgctata atacctcaga aatagcagat
    12601 attataattt tatatgggaa gtggtattgt ggaacttatt aaagatattg tgaaatattt
    12661 aagtagtgga ttagcagaat atttcttaga atgtaaagtt agtgacaaaa gacaagaata
    12721 ttttagtaat aaaggtggaa agtgggcgaa attctgtttc tatatgtcgt taatagtttc
    12781 tatcattcta ataatcatag gtattattgg gttagtaaaa aagaaaagta taagtatttt
    12841 ctttttgatt gcaggtatgg gtatcgcttt gtttatgctg gttgactggg gaacatcaaa
    12901 aaaataattt tctatgcaat acagtttaat aaattttaaa gtaattagtc ttaggattga
    12961 ctgctttttc ttacctaaaa ttagattgga gtgatgaaat gaaatgtata atgcttcatg
    13021 gactgggaca atcttctgaa agttggattg atactgtaaa agtattcaat gatgattttg
    13081 aagttttatg tccgaattta tcagattggc tttctgggaa agcaccatgc tatgatacat
    13141 tgtatcaagc attggaaaca tactgtgaac agtttgaaga acagctgaat atttgtggac
    13201 tttctttagg tggtattctt gctatgcagt atgcaataga acacccagaa aaagtgaact
    13261 cattagtttt aataggaaca caatatgaaa tgccaaagta tctgttaaaa atgcaaaatc
    13321 tgatattccg tattatgcct tgttcagcat ttgaaaaaat gggctttcag aaaacagatt
    13381 ttatcaatct atgcaaatct atgacagaac ttaattttaa acatgaactg aaaaaaatat
    13441 catgttcggt acttgttgtc tgtggcgata aggataagat aaataaaaga gcttctttaa
    13501 aattgcaaca acagttagaa aacgcagaga tagctattat tgaaaacgca ggacatgaag
    13561 taaatactga taatcctaca ttactggggc aaaagttaaa tatatttttc agacagtacg
    13621 tctgatacac aatttcatat ccatatatac aaagcagtta gttttaggat tgactgcttt
    13681 tttcgtacct aaaattagat tggagtgatg aataagatta tcggtacata aattttattg
    13741 tggtgctgat atgcgaatgg tataattgag atatgaaaaa agataatata gattaggagt
    13801 tacgctatgg attgtattat acgggaaatt aaaaaggaag aatatggttt actcaacgac
    13861 tttttgtatg aagcaattta tattccagac ggtgttgaac caccacctaa atcaataatt
    13921 aactgtccag aattacaaga atatgttttt gactttggaa agcgaaaaga tgataaagct
    13981 ttagttgcgg aaattcaagg aaatgtcgta ggcgctattt gggtgcgaat aatgaacgat
    14041 tacggacaca ttgacaatga taccccgtcc cttgctatgt ccgtttttcc aaagtataga
    14101 ggacaaggga ttgggactgc attattacaa caattattgc tagttgaaaa atctcttggc
    14161 tattcaaaaa tttccttatc tgttcagaaa aataattatg cagtaaaaat gtataagaaa
    14221 gcaggttttc ttgttgttgc tgaaaacagc gaagaatata ttatgactgt aaatctataa
    14281 caaataatct agtttacaat ttcatatccc tacacacaaa gcagttagtc ttaggattga
    14341 ctgctttttt catacttaaa tttcagattg gagtgattac atgaacgata tttttaaaga
    14401 tatgcaggca aaagtcggct gtgattatat ttccgacctg ccctcttaca agcgtaaggt
    14461 gtggcaggaa atgaaacggc tgaaccctgc cgactatgaa gaaagacagt tagaagattt
    14521 ttctaaatat gtgtttggta tgtcgtacca gaccttaaaa gatgtgatga aacaacagaa
    14581 aggacgtgag gaacaatgca ggaaacaagg gtgctggtgg aaacgagagg aacaactggc
    14641 gaagaaacaa tatcatactg gtttgaactg ccgatagatg ttgccgagtt tgaagaaaag
    14701 ttaggtgtcg gtgcagaaag tcaagattac cgtattatcg aaaaggtact gccttatgct
    14761 gatgaagtcc acgaacatac cagcgtgtat cagctcaacg aattagaatt tatgtaccgc
    14821 cagctttcaa gtgatatgca ggaagaatat gtatcactac ttacggtgtt tgagaattta
    14881 gaagcacttt atatttgcag gaacgtgatt accgtttatc cagactgcaa aagcatgata
    14941 gatgttgcaa ggcaaaagct gatgaataac ccgacgttca aacatttatc cgaggactgt
    15001 caagaatatt actttgactt tgaagcctac gcttttcact tgcaggaaca cgggaaattt
    15061 ttagtaacag aacacggtat ctttgaactg ccagagtagg aaatggggtg gtgcttatga
    15121 ttgatgatat ggcggtttac attgctaatc ttggcaaata caatgagggt tatttagtcg
    15181 gtgcttggtt cacgttcccc attgacgagg aagatgtaaa agaaaaaatc ggcttgaatg
    15241 aacagtatga ggaatacgct atccatgata ccgacaactt ccccattgcg attggcgagt
    15301 atgtttccat tgaagaactc aatgagatgt atgaaatgat agaggaactt cccgactata
    15361 tcgtagaatg tctggacgaa tttatcagcc actacgggac gctggaagaa gtcgtggaac
    15421 ataaggacga tatttattat tatcccgact gtgaaaccat gacagatgtt gcctactact
    15481 acatagacga attgcaggca cttggggata ttccacccag cttacagaac tacattgact
    15541 atgaagccta cgggcgagat ttggatatgg gcggttgctt cattgaaaca agccgaggta
    15601 tgtgcgagat accatattaa cgctggaaga tcagcaacaa gctttgttgc tactgacagc
    15661 gtatttctct tttaagggac agtctgaaaa tggctgtcct tttctattat gaaaggagtt
    15721 gaaagaactt gaaaaagatt aaatcttata cggatatctg gaacgtggaa aaagtcttgt
    15781 atgcaatcaa tgactttaac ttgccctttc ccgttacttt tacacagatt acatggtttg
    15841 tgattacgga atttatcatc attctgtttg gagatattcc cccactttcc atgattgagg
    15901 gagcatttct caaatacttc ggtattcccg ctgctctcac ttggtttatg tcgcagaaaa
    15961 cctttgacgg aaagaagccg tacagctttt tgaaatcaca gataacctat gcgataagac
    16021 cgaaaatcac ttatgcagga aaagccgtaa aactgcataa gcagaccttg aatgaaacaa
    16081 tcacggcagt aaggagtgtg aacgatgttc ccgataaaat atattgacaa taaccttgtc
    16141 tggaacaagg acaatgaggt attcgcctac tatgagctga taccgtacaa ctattctttc
    16201 ttatccgcag agcgaaaatt tatcgtgcat gacagctttc gccagcttat cgcacagtcc
    16261 cgtgagggaa aaattcatgc gttgcagatt gccacagaaa gctcaatccg cagtatgcag
    16321 gaacagtcaa agaaactggt aacgggaaaa ttaaaggaag ttgcctatca gaagatagac
    16381 gaacagaccg aagcgttagt atctatgatt ggggacaatc aagtggacta ccgctttttt
    16441 cttggcttta agctcatggt tacggaagaa cagcttaatc tgaagaacat taaaaaatcg
    16501 gcgtggctga cgttcacgga atttctccat gaagtgaacc atacgctgat gaatgatttt
    16561 gtttccatgc cgaatgatga aatcaaccgt tacatgaaaa tggaaaagtt actggaaaat
    16621 aaaatatcaa ggcgttttaa ggtgcgtcgc ttggaaatca atgattttgg gtatctcatg
    16681 gaacatcttt acggcaggga cggtatcgcc tatgaagatt atgagtacca gctaccaaag
    16741 aagaaattac agaaagaaac gctgataaaa tactacgacc ttatccgtcc gacaaggtgt
    16801 gtgattgaag aaagccagcg gtatttacgc ttggagcatg aggacaagga aagctatgtg
    16861 tcctatttta ccgtcaatgc gattgtaggc gagcttgatt ttccgtcgtc tgaaatcttc
    16921 tatttccagc aacagcaatt cacattcccc gttgatactt ctatgaatgt agaaatcgtg
    16981 gagaacagaa aggctcttac cacagtaaga aacaagaaaa aggaattgaa agaccttgac
    17041 aatcacgctt atcaagcagg aagtgaaacc agctcaaatg tggtggacgc attagacagc
    17101 gtggacgagc tggaaacgga cttagaccag acaaaagaaa gtatgtataa actctcctat
    17161 gtagtgcgtg tatcggctga tgatttggac gaattaaaac gccgttgtga tgaagtcaag
    17221 gacttttacg acgacctcaa tgtaaagctg gtgcgtcctg ctggggatat gctggggctt
    17281 cattctgaat ttcttcctgc cagcaagcga tatatcaacg actatgtgca gtatgtaaaa
    17341 tcagacttct tggcagggct tggttttggt gcgacacagc agttagggga aactacgggt
    17401 atctatatgg gctattccgt tgatacggga agaaatgtgt acctgcaacc gtctttagct
    17461 tcgcagggcg taaaaggcac agttacaaat gcccttgctt ctgcttttgt cggttcgctt
    17521 ggcggtggta agtcgttctg caacaatctt ctggtgtatt attcggttct ctttggggga
    17581 caagcggtta tcttagaccc aaaatcagag cgtggcaact ggaaagaaac gctcccagaa
    17641 atcgcccatg aaatcaatat tgtaaatctt accagcgata aagacaatgc agggcttctt
    17701 gacccatttg tgattatgaa agataaagag gacggagcga cgctggcaaa agaaatctta
    17761 acatttttaa cgggtatctc tacccgtgac ggagataaat tccctgtgct tattagtgca
    17821 atcagcaagg tatctgaaag agaacacaga ggactgttaa atgttatcac agaattacgg
    17881 caagagaata caccgattgc gaaccatatc gcaaatcata ttgccagttt tacaaattat
    17941 gattttgcac atctgctgtt ttcggacggg acagtagaaa atacaatcag tctggataac
    18001 cagctcaata tcattcaagt agctgacctt gtactgccag ataaagatac cacttttaac
    18061 gaatacacga ccattgaatt attatcggtg gcaatgctga ttgtgattag tacctttgcc
    18121 cttgatttta tccattcaga cagaagtatt tttaagattg tagaccttga cgaagcgtgg
    18181 gcgttcttaa atgtggcaca aggagaaacg ctctctaaca agctggttcg tgctggacga
    18241 gctatgcagg caggcgttta ttttgttaca caatcttctg gcgacgtgtc aaaggaaagt
    18301 ctgaaaaaca atatcggttt gaagtttgcc tttcgtagta ccgacatcaa cgagataaag
    18361 cagaccttag aatttttcgg cattgacaag gacgacgaga acaaccagaa acgcttgcgt
    18421 gatttagaga acggacaatg cttattgcag gacttatacg gacgtgtcgg tgtggtgcag
    18481 atacaccccg tctttgaaga actgctacac gcctttgata ccagaccgcc cgtacagaga
    18541 aatgaggtgg agtgatgaaa gaaaggataa aaggtgcgtt cacaaaaaag aagattttcc
    18601 actttctcaa aatgactctg ttcgtggcgg cactctccct tatcctgctt tcacttttgg
    18661 ggacggtggc tcatgcgacg gggcttgtgg acgataccat aaacgcagaa aatctttatt
    18721 caaaatatcc cctttccaac taccagcttg atttttatgt ggataatagc tggtcgtggc
    18781 ttccgtggaa ctggctggac ggtatcggaa aatcggtgca gtacgggctt tactgcatta
    18841 ccaactttgt ttggacgata agcctttatt taagcaatgc cacgggctat gtggtgcagg
    18901 aagcctataa attagacttc atcaacgata tggcagacag tatcggaaag agcatacaga
    18961 cccttgcagg cgtaacacag aacggctttt cttctacggg cttctatgtt ggtttcctgc
    19021 ttctcattat cttagtggta gggctttatg ttgcctatac gggactgata aaacgggaaa
    19081 ccagcaaggc acttcacgct gttatcaact ttgtggtggt gttcgtgctg tccgcttcgt
    19141 ttattgccta tgctcccgac tacatcaaga agataaatga gttttcatca gacatcagta
    19201 ccgcttcact tgacttggga acaaaaatca tgctcccaaa ctctgacagc gagggcaagg
    19261 acagcgtgga cttgataagg gacagcctat tttctattca agtggaacag ccgtggttac
    19321 tcttgcaatt cggcaacagc aacgcagaag aaatcggggc agaccgtgta gaagctcttg
    19381 tatcggcaag cccagaggac gaggacggaa agaccagaga ggaagtcgtg aaaacagaaa
    19441 tcgaggataa cgacaacaac aatctgacga taccgcaggt ggtaaaccgc ttgggtatgg
    19501 tgttcttcct cttgttcttc aacttaggga taacgatatt tgtattcttg cttacgggca
    19561 tgatgttatt cagccagata ctttttatta tcttcgctat gtttctgcct atcagctttt
    19621 tactttccat gataccgagc tatgaaagca tggcaaagca ggcaatcgtg agggtattta
    19681 ataccataat gacacgggca ggaataacgc tcattgtaac ggtggcgttc agcatttcca
    19741 gtatgtttta taacatatcc acggactatc cgtttttcat ggtagctttc ttacagatag
    19801 tatgtttcgc tggtatctat atgaagctgg gcgacttaat gagtatgttc tcacttaatg
    19861 ctaacgacag tcaaagcatg ggacgaagaa ttttccgcag accgtatctg tttatggcac
    19921 acagggctag acgaatggag cgacgcatgg caagggctgt gagtgccggc ggtgtttcgg
    19981 gcggtgtggc tggtgcagtt gtcggaaatg ctgttgcgaa taaaagagca gaaagaaaaa
    20041 atacggcttc taaagacaat cggggcaacc ccacttccag catgggacag cgtgcaggct
    20101 caaagatggg tgctgtctta gatacgaaaa ataaagtgaa agacaaggca aatgctgtca
    20161 aagagaatat caaggatatg ccgacacaga ctgcttatgc ggtgtattcc gcaaaggaaa
    20221 aggcaaagtc cagcgtgtcc gactttaagc gtggcatggt gcaggaacag cagttcagac
    20281 agacgggacg tttggaaaag caggaacagc atagacagaa tatcgctgac aaacgtatgg
    20341 agcttcaaaa ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa
    20401 cgggagctac ccgtccccat gagcgaccag ccacagcttc aaagccgagt gcggaaaaaa
    20461 tgcaggaagt caaacgccct gccacggctc caactccgaa agcaagtgag ccagtcaaga
    20521 caactgttat caaagagcgt ccgttatctt ctggtgcttc tgataagaaa gcaacccagt
    20581 cggcacagat agcacatagg caaaatgtag aaaaagtggt atcgcaggaa acacgccaga
    20641 attacaccaa agaccgcagg acaaaggttc agcagacgca aaccgtccaa aagaaccagc
    20701 agaccacaga gaaaacccgt aaccttgtaa cgaagaaagg acagaagaaa aaatgaaact
    20761 gaaacatatc gctatcattg gcagtctgtt tcctattctc ttttctctgg tgcttttctt
    20821 tggggtgctg attagtgcgg acagcgacga cgagaacagc aatttttctt ctgggattac
    20881 gggtatgaac ctatccgcag aagtcttgaa acatcagcct atggtagaaa aatacgccag
    20941 agaatacggc atttctgagt atgtcaatgt gctattggct atcattcaag tagaaagtgg
    21001 cggtacggca gaagatgtta tgcagagttc ggaaagtctt ggtttaccgc ctaattcctt
    21061 agatacagaa agttcaatca agcaggggtg taagtatttt gcgtccctgc tttcttcctg
    21121 caaaaatcaa ggtatcgacg atttgaatgt agcgatacag tcctataact atggcggtgg
    21181 ttatgtggga tatgtggcag gaaaaggaaa gaaacacacc tttaaccttg cagagaactt
    21241 cgcccgtgaa aaatcgggtg gaaagaaagt aacctacacc aacccgatag ccgttgcgaa
    21301 gaatgggggc tggcggtatg gctatggaaa tatgttctat gtggaattag tcaatcaata
    21361 tctgaccgtg gcacactttg ataatgcgac ggcacaagcg attatgaatg aagcgttgaa
    21421 atatcaaggc tggaagtatg tgtatggtgg cagtaacccg aacacttcct ttgattgtag
    21481 cggacttgtg caatggtgtt atggaaaagc tggtatctcc ttaccgagaa cagcacaagc
    21541 ccagtatgac gcaacccaac atcttccact ctcgcaggca aaggctgggg acttggtatt
    21601 tttccattct acctataacg ctggttcgta tgtaacccac gtcggcattt atgtaggaaa
    21661 taaccagatg taccatgcag gcgatccgat aggatatgca gacctaagca gtagttactg
    21721 gcaacagcac ttaatcggtg caggacgagt aaaacaatag aaaggacttg aaaatatgtt
    21781 taagaagaat aagaaacaga cagaaactct caaagaacca aaagaaaaaa aggtgcgtac
    21841 tgttaaggta ggcacacata agaaaaccgt gattgcgttg tgggtggtgc ttatcgcaag
    21901 cgtgagcttt ggagtgtata agaattttac tgctatcgac cagcacacca cccatgaaaa
    21961 agaagtgata gagcttcgct tgcaggacac caacgggata gaaaatttcg tgaagaactt
    22021 tgcgaagtcc tattacacat ggaataacag caaagaagcg attgaagcaa ggacgcaggc
    22081 aatcagcggt tatctgacaa aggaattgca ggacttgaat atagatacca ttagaaccga
    22141 tataccgacc agctccacag ttacagatgt gattgtgtgg catatcgaac agtcgggaac
    22201 ggatactttt tctgctacct acgaagtaga tcagcagata aaagagggag aacagacaac
    22261 agcggtcaag gcaacctata ctgtgaaagt ccatgtggac gctgacgggg atatggtaat
    22321 cgttcagaac cctacccttg caccagcagt tgaaaaatca gactatgagc ctaagacacc
    22381 agaagcagac gcaagtgtag acgctgatac cgtcaacgac gctacctctt ttctggaaac
    22441 attttttaag ctctatccaa cagccacaga aaaagaactt gcctattatg tatcgggaaa
    22501 tgtgcttgaa cctatcggca gggactatct gtattctgaa ttggtaaacc ctatctttac
    22561 aaaggacggg gacaatgtga aagttaaggt tgccgtgaaa ttccttgata atcagacaaa
    22621 ggcaacgcag gtatcacagt atgagcttgt gctatataag gatagtaact ggaagattgt
    22681 aggataaatc acaagaagta gtttgcatgt catagaaata tgatttgcaa actacatttt
    22741 catcgaatta tgttcacaaa tagttctgaa tacgctaaaa tagtaattca cgcaaaaacc
    22801 gcaatatata ttaaaacttg aagaaaacta tttattataa tctaagctga aaggagggtg
    22861 ctgtaatggt caataaggtt gaaaacatta taacggttat caattacata gaaaacaatc
    22921 tcacagaaaa actgaactta tcctctattg cgataggtgt tggttattca aaatatcatt
    22981 tgcatagaat gtttgtcgaa actgttggat tgtctataca tgactatgta caacgcagac
    23041 aattaacaga gtctgcaaaa cttttagtgt tttctgataa atctattctt gaaatttcgc
    23101 ttattgctgg ttatgaaagt cagcaggcat ttacaaatat ttttaagcag atgtataaga
    23161 aaacgcctaa cgaatatcga atgaacgaag aattttatcc gttacagctt agatttgatt
    23221 tgatacagac agttaaacaa aaattatcac aacaagaaat ggaagaaaat atcagatttg
    23281 ctacaacaac agatatacct gcctgcctag aactagctta tttagtaata gacggtttcc
    23341 caaatttgaa cgagaatgaa tacttaacag aattagaaag aggtatttta gagcaaagag
    23401 tgttgatttt aactgataat actgttgcta ttgctttgat gtctattaac tatcatacgg
    23461 gaagtattga tttctttgga gtacacccgt tatatcgtaa atgtggtatt gcaaaattat
    23521 ttttaaataa ggttatgaat gaacttttag ttgataaaga catttctgta acaacgtttc
    23581 gtgagggcga caaggctgat acggggtatc gtaaggctta caaagaatta ggctttgcag
    23641 aagcagaatt attagttgaa tttggttatc ctacacaaaa attagtatta ttttcaaaaa
    23701 atgggggcag tacaaatgaa tagtaatcct cttgcaaaag attttaatac tgggtcttta
    23761 ttaaaatttg ctttcccgac catatccatg atgttattta tggggcttta cacaattgta
    23821 gataccatat ttgtatcacg atttgtcaat acaaatgctt tatcagcaat caatattgtt
    23881 tgtcccgtta tcaatttaat agtaggattg ggaacaatga tagctacggg tggtagtgcc
    23941 atagttgcaa gaaaaatggg ggcaggagaa cccaaacgag caaaacaaga ttttacacta
    24001 cttattttat ttggttttat tttgggaata gtaattttca ttctaggaat tatttttatt
    24061 gacgaaatta tatatgctct tggagctgat aaggttttat ttccatattg taaagactat
    24121 ttaactatta ttttactgtt tacacctgca agtatgttgc aggtactatt tcaaaattta
    24181 tttgtaacag caggaaaacc aactttagga cttgttcttt ctgtaagtgc tggtgtaatg
    24241 aatatcgtat ttgattatct tttcatggtt atatttgaca tggggattgc tggtgcagga
    24301 ttgggaactg gaattggtta tttgatacca gcaattacgg ggctaattgt cttttgtaaa
    24361 aaatcgggaa cattgtcttt tgtaaaacct attatagaca ttaaagtact tggagaaagt
    24421 tgtataaatg gttcatcaga aatggttagt cagctttcaa cggcaattac tacattcttt
    24481 ttcaatcgca caatgatgaa gttattaggt gtaaatggtg tggcttctat taccataatt
    24541 atatatacac aatttttgct tacaacatta tttattggtt tttcaatggg ggttgcccca
    24601 gttattagct ataattttgg agcaaacaat tattatggat taaagaaaat atataaaatc
    24661 tgtatttctt tcatttttgt aggttcatta tttatttttg cgttatcctt tataaatgga
    24721 aatagtttgg tacgtttatt tactgggaca aatgaagcgg tatataaaat tgcaataaac
    24781 gggtttatga tatttgtatt tagttttctt ttttcgggtt tcaatatatt ttcatcagca
    24841 atttttactg cattatctaa tggtaagcta tctgctttga tttctttttt aagaacattt
    24901 ggctttataa tgataggctt gttaataatg ccacagttta ttggaataac aggtgtatgg
    24961 ttggctgttc ccgttgccga aatattaaca ttatgtattt cattatattt gaattttcgt
    25021 ctttggcttt cgtggaacaa tcagaataat ggtatgaaaa aagtatagaa aggttattat
    25081 gataggactt aaaaaacgag atattaaaaa agccttgaaa gcaggtgcaa aagctggcgg
    25141 tgtatcattg gctgatgttc gggctgatat tgaagcgacg attgatgaag ctatgaacag
    25201 caccgaccca gaagtgcagg caaatttcaa aaagtatttt ggaaataaac gccctacccc
    25261 agaggaatat atttacaaga ttaccaaaaa gacaaagatt tgaaaaaagg cttgctccat
    25321 acatattgta tgatctgcaa gccttaatct atttcaaagg aaatgtaaca gtaaaggtag
    25381 caccgccacc agcagtatca tggatattga tagtaccgtt cagcatttga gttaattctt
    25441 tggcgatact tagtcccagt ccaaaattcg atttgttcgt atgggacttg tcgccactat
    25501 aaaagcggtc aaagatatag tgcttatctt catcagaaat gccttgcccg tggtctacca
    25561 cagaaaaagc aatattcttt tcattggcga ttacttctat ctctatcaag gaattattct
    25621 tggaatgttg aatggcatta tccataaaga cacataagat ttgaaataat cggtctttgt
    25681 cggtaagcat ggcaggataa ctttcctcgg ataaatgcag ttttagttct aaattctgct
    25741 tcatgcagac gggttcgtag gtttcatata acgtaatgag caaggtatct acattgattg
    25801 tgcttttatg caatgtccat gttttagcgt cggaagaagc caaaagcaac atatctttga
    25861 ctaaacggga taaccgcata cattcaaaat ctatggtctg tacggcttgc tttgcctgct
    25921 catttaagga aacattatca agaagcatat ccgtgttgga aatcataaca gcaagtggag
    25981 attttaattc atgggacgct gttgcaacaa aatctttttg gctttgcagt attcgttccg
    26041 ttggagcaaa cgactttttc agcagataac gggtcagcac gataacaacg atacaagccc
    26101 aaaaccagat aagaagatat ataggcaatg tcttttgtaa aatatccgtc aaactggctg
    26161 tctgataaag aaaggttgca tgatacacgg tatcatttgc tgttctaatt gtagcaggaa
    26221 taacaaaata agtatcgtga tgtgttcctc ttatttccaa aaaaccgcct tgtgaagttg
    26281 tgttgctttc tgtttgtgat aatggctgtg tagaaatctg cttttctaca tcatgcaaca
    26341 gattatccgc agaagttgga aatgggaaat cactctgata aatggtattc ccccttgtat
    26401 cagtaattaa agaaaaaata tgataacttt cttcgtaggt tttgagtgct ttgtccatat
    26461 ccgaagaagc actttccact tgataaatca gcaaggttgt caatctctga aataaggtgc
    26521 tttcgttgat tttctctgta tgaactgtgt tcgcaacgac aaaggaaatc acaagggtaa
    26581 taatcagcat aacagaactt gcaaacagaa tatgaagttt tcggtataaa ttatttaaca
    26641 tgtttctttc tccagtttgt accctgcacc gtagaccgtt gttattttac aggaactttt
    26701 taattctttt aggcgttttc gcagaaaaga aatgtaactg tctacattgc cagtttctac
    26761 ctcggaagaa ctgccccaga ttttaaggat tagctgttca cgggaaaata gtgtttctgg
    26821 gttctgcata aatacaaaag tcaattcggt ttcctttgaa gttaataaaa tagaattgac
    26881 gggtgtagat aaggttcggc tgtctttatc gaaagttaaa tctccatatt gtaatttaga
    26941 cgataactgg atgttggcag gtcgccttgt caatgctcga actcttgcaa gcaattcttt
    27001 gatatgaaat ggctttacta agtaatcatc agcaccgccg tctaaaccct caacccgatt
    27061 gtcaagggta ctcatggcag taatgataag gataggaata gatattcctt ttctacgcat
    27121 agattttatg attgtcaaac cgtcaataat cggtaacata cggtctacaa tggcgaggtc
    27181 ataagcatag tctgtattta aggcatacag catagcggtt tccccgtcat tgcaacaatc
    27241 aaccatatag ttttcttttt ccagcgaaat ttttatattt ttacataatt ctaaatcatc
    27301 ttccagaaga agtattttca taatgtatca atccttttct atatagatta tagacagtat
    27361 aacagaaaag tctgtaaaaa tcctctaaaa aatgatgatg attttcttgc ttttttacag
    27421 gatttttaga atggtttgcc tttacaatag gggcatatcg aacaagaaag gtggaataca
    27481 aaaatgaaaa aaacaaacat cagtaaattc atagcagttg gattatgtat ctgtgcattg
    27541 acggggtgtg gtgcaagtcc agatgagaaa ccagatacgt ctaatcctat tgtcaattct
    27601 aatacgaatg aagaaaatgc aaatggttct tcagaaaata aaggaaacga catattagag
    27661 tctgctaatt tgataggaag tgttttggaa tttacagaca atggttgttc cgtaagtcaa
    27721 gcaaaagaaa ctgatggtgg agccagcctg caaatcgaag cgtcgggaat ggaaaataaa
    27781 gacaatactg tttctgtaac ctatacccca gattgtgaat ttgttgttgc gacactaaat
    27841 aaacagttgg gttctgttac gaatataaca acgggttcta tatctgatgt aaagaaacaa
    27901 tcaaatgtat atctctatgg agagtttgca gatacacttc atttcaatgc aacaaaggtt
    27961 gtcgtagctc gttgggaata gaggtgtgaa gtatgaagtt ttatcaactt ggttttcgct
    28021 atttacagcg aaaaaaagga aaaaccattc ttttgttgat tgttttgata cttgtaaaca
    28081 gtatgatttt aggtacaagc atgattttaa ggactacaaa tgagtccaaa caagccatgc
    28141 aggaaaagac aaattctaag attgtagctg aaattacaag tgaggacagc aagataaccg
    28201 agaatgaaat aaacaagatt gaaacgctgg aagatgtttc gtctatcaac cgtatcggca
    28261 gacaagaggt atttccgaat aattttgctc ccgttactct caatacttct acgaatgagg
    28321 aaaatttgaa aatagcgttg ctctcctatg atgatttgga aaaagacagt cctttttcag
    28381 aaatgcagta ccgcttagct tctggcgatt atatcaaaca tgagaaaaaa ggtgctatta
    28441 tcaatgcgaa ccttgcaaat caaaatggac taaaggttgg agatacgatt gaactaggta
    28501 cagaaaacgg aacaaaagta tctgttcaga ttattggaat tttcatgtcc gcaggaaatg
    28561 tggaaaaaga ccagccgtca gaaactaccg cagtcaatcg aatagaaaat caaatcttta
    28621 ttgacaacag cacttacaaa gaattgttca aagaaactga atttgaaaaa ctctgcgttt
    28681 attcacaaag accagaaaaa ttagatgtgc tggaaaccag cctgcaaaaa atattcggaa
    28741 atgatgtaga gcttagcact tccgataccc tttatcaaca gatgtctgct ccattagagc
    28801 aaatcagtaa ggtggcaaat ctcatgctga tacttacctt gataactggt acggtggttg
    28861 tttccctgct gttatgtatg tggatgagaa caagacaaaa ggaagtggca atctttatga
    28921 gtctaggtaa agcaaaaagt gaaattttcc tgcaaacttt cttagaagca ggtagcgtat
    28981 ttacgctttc ggttttaggt gcaacagcca ttggtaaatt gttctctggt gttttgcaaa
    29041 atgtcattac tggttcagaa agcgtaacag aaaatctgaa agtggtgttg cagatacaag
    29101 atattggaat gttgttcctt ttaggtggtg cagttattct ggtggcattg tgctgttcca
    29161 ttcttcccat attacgagca aacccaaaag atatattagc gaaaatggag ggatagtaca
    29221 tgaatttttt tggattggca aggcgttctg tgttcagaaa gcccgtaaaa agtattctct
    29281 tattgttgat tgtttttgca atcagtaccc tgcttcttgc aggtgtggcg agcaaaaatg
    29341 ccagtattga ggtgcaggat aaaacaaggc aagcgattgg tgcaggtttt cttttagaaa
    29401 aaaatgcgga gtatcgaaca aaacgaatta gtgaattatc agaaaaaatt ggcgaaaagg
    29461 atggagaatt agaagggtat catcaagaga aagtagttgt taatggtgca gaaggttgga
    29521 gggggtggac aacaaacgaa tttgaaaccc tagatataaa ggatattgag aaactggcaa
    29581 aaacaaatgg aatcgctgat tacaatatta caacagcaat cacagccgta aaccctgtaa
    29641 acttccaaag aattgaagat aaagatgtag accagagtgt tgacgttggc ggtgtaagcc
    29701 taattggaaa caaagatatg aaacttgaca gaaatgtttt atctggaaac ctgcatatca
    29761 aagagggacg aatgataacc ccaaaggata aagatatgtg tgtcatttca gaggaacttg
    29821 caaagcaaaa taatctgaaa atcggcgata agatttcttt taatgattac cacgatactg
    29881 aaaattcaaa ggtatcagaa gctgagattg ttggaattta tcaagtggat caaaagatgt
    29941 ccccacttat gcagggagat acttaccgtt cggaaaatgt aatatttaca gatttgagat
    30001 ttccagaaaa ggcagagggt gaaacaagtc cattatatga aagagcctat ttcaaagtag
    30061 aagatgtgga agcttacgac gaagtaaaag aaaatctgca aaaagtagac atcaactggg
    30121 agcaatatga tttgattgac aataacggaa attctgaaac catgtcctca aacttcaatg
    30181 atttagcaaa agtaagcgaa atgatgatat tggtaatctc tgttgcaagt tttgttatcc
    30241 ttgttttcgt atttcttttc tggctgaaaa atcgagtgca ggaagtcggt attttcttat
    30301 cactcggtgt tccaaaactt agaattatcg gacaaatttg gagcgaagcg attatgattg
    30361 ctgttctttc ccttatgtta tcctttgctg tcgctccagc cgtttccaaa gtaactgcaa
    30421 attatctggt atcacagcag gttcagcaaa tgcaggaaga agaaaaaaat aacgagggaa
    30481 aagtatccac agaatatgtt gcaccaaagc aggacgtgca gaatatcagc gtagaggtta
    30541 cgccagaaat gtatctgttg gacggtgtaa gtgttcttgt gttgataacg gcttctgttc
    30601 tggtatctgg aattgtaata ctaaagagaa acccgaaaga tattttatcg gaaatgagtt
    30661 aggaggacga cgaaaatgtt agaagtaaag aatgtaagct atgcttacaa aacaaaaaaa
    30721 gataaaacca ttttgaataa tgtatctgct actttcgagg agggtatttt ttatactatt
    30781 gtcggtccga gtggtgcagg aaaaacaaca ctcttatcgt tgttagcagg cttagacaca
    30841 gcggtaaaag ggactgtcct ttgcaatggc gaggacatta caaaaaaagg gctgaattac
    30901 cacagaaagc ataatatctc attggtattt caaaattata atctgattga ttatttgaca
    30961 acggttgaaa atgtcaaatt aggtggtagt ggaaatgctg aaaaattatt ggaagaagtc
    31021 ggtattggaa aagaatactg gcagagaaat gtattgcaac tatctggcgg acagcaacag
    31081 cgtgtagcga ttgcaagagc attagccagc gacgctcatg tacttttggc tgatgaaccc
    31141 acgggaaatc ttgacgaaac taccgctgat gaaatcattg ctttgctgaa aaagactgca
    31201 catgaattag gaaaatgtgt tgttgtcgtt acgcatagca agcatttggc agaagaagcc
    31261 gacgaaatct tagaaattaa aaacggtgca atttcttttc tgtctgaata aattagaata
    31321 gtgccgtaaa ggagttgtca ttatggaata ttttagatat acctcgacgc aatcagtttg
    31381 aacctaacgg catttttata acaactgaat acagcacagt tttaaaagct cgtacagact
    31441 atatgttttg tgcgggcttt tttcatgcca aaaaaaattt ttttaagttt tttttgattt
    31501 tggttacgct gtcccccact ttcaacgcgg tatatatgaa aaggggcaga cacctctttt
    31561 cgactagcga aaggaggtga ggaaaatgaa accgtcagaa ctacaagaaa aaacctgtca
    31621 tcaattcgat tgtgctatta aaacagttat acgtcataga gcgattgact tctatcgagc
    31681 gatacgtcgt tcagagaaaa aagaaatgcc attcgccgaa attatggacg acctcttaaa
    31741 ttccataggt acagaagata catattctac ggattatgtt gcagtatttg aagtctgtgg
    31801 agaaaaagta tcaatcgaag atgaacaact ggctcgtgca ttgaaagcta ttgaagaacg
    31861 aaagcgtaat atcgttttac agtatttttt cattggggac acggacactt acattgggaa
    31921 aatgcttgac tgttccagag cgaatgtaac gaagtgcaga atacaagcac tagacaagtt
    31981 aaagcaactg attgaggagg aaaaagaaaa atgccaaaag agctgacacc gactttcacg
    32041 gttatttcca aagcctgcga cggcgacgaa acagcaattt caaaaatctt ggaattttat
    32101 gacccatata tcaatcagtg ctgtatgcgt ccattttacg acgataacaa caacttacgt
    32161 cttgcggtag atttggaaat gaaaggtttt atcaaagaag cgattattcg taaaatctta
    32221 aactttgata ttaaacgctt ctaattcata tcgggattga ttatgcccgt ctggtctttg
    32281 acaactgaat aaagcagaca gatacgtttg tgagatagca agccacgggg actgtacgcc
    32341 acgacctttt caaaagaaag cgagcgaccc acgcagtgat ccgagagcga ccgttggaaa
    32401 agtcgctttg ccacgaccta tcctcacaga ataatgatac gtccgcatgg tgcggttctg
    32461 cccacaggaa tgggaatagt tgagatacta acggagcttt ccaaagccgt ctgcctgctt
    32521 agaaattaca agcaaggggg aattatatat ggataaaaaa ctaatacgat atagtatgca
    32581 gattgccatg ttgaaccaac ttcttgcccg taaaatgatt tctgaaaaag aatatgcttt
    32641 agtaaaatca aaacttatgc aagattataa aatcgtttca gatataaccg cttgaattgt
    32701 atatttgaat attgtataat aacactacaa aaggaataca aaatgcaaat ataaaaggag
    32761 tggttctgtg aaagaagtag aagtaatcaa agcaagaaat gatttgcaaa ctcgtacacg
    32821 aggaaaaacg attgaactta tgcgtgttgg tgcatattgc agagttagta cagatagtgc
    32881 agaccagcta aacagttata agtcgcaggt agcttactat acagatatga ttaagaagaa
    32941 taaagaatgg gtacttgcgg acatatatgc tgatgaagca attacaggta cacaagtggc
    33001 aaaaagagaa gatttccagc gtatgataaa tgattgcatg aatggagaaa ttgatatggt
    33061 tattacaaaa tctatttcca gatttgcaag aaatacgcta gataccttaa aatatgttcg
    33121 tatgttaaag gaacgcaata tagcagtata ttttgaagat gagaaaatca atactttaac
    33181 tatggacgga gaattgcttt tagtggtact tagttctgtg gcacagcagg aagttgaaaa
    33241 tatatctgct aatgtaaaaa aaggtttgaa aatgaaaatg aaacggggag agctggttgg
    33301 ttttcaaggt tgtcttggat atgattatca caaagacacg aaaacaatta ccgtgaatga
    33361 agaagaagca gaaattgtac gttatatttt caatcggtat attgagggtg caggtggttc
    33421 tgttattgca catgagcttg aaaacttagg atataaaaca aagtacggaa gttctacgtg
    33481 ggtgcaatcg acagtcattg gcattatcaa gaatgaaaaa tataaaggcg atttattgct
    33541 gggaaaaacc tttacagtag acccgatttc aaagagaaga ttggagaact tcggagaaga
    33601 agataaattt tatattcgta atcatcacga accgattatt agcgaagaag tttttgaaaa
    33661 agcacaagaa attcttgcta gaagaaatgt taatagagga aagattggag agggtaacac
    33721 aaagagagaa aaatatagta ggaaatatgc tttcagttgt atgctggaat gtgggttctg
    33781 tggtggcact ttaacaaggc gtaattggca cagcagttca cagtacagca aagttatatg
    33841 gcaatgtgta acagctacga agaaaggaaa gaaattttgc aagcatagca aaggtattcc
    33901 agaaacagct attgaagaag cgtttgtaga aagttatcgt ttattgtgtg atgataataa
    33961 agatgttctg gaagaatttt tgcagagaat ggacgatacg cttagtagta gtgcggtgtc
    34021 gaaacaactt gcaaaagcag aaaaagagat agacgcatta gagaagaaaa agagtagact
    34081 tgtggatatg cgtctggaag aaattattga taaggaaacc tacgaaagca aatatgatga
    34141 cttagtaagt aaacaagaac agcttattga agaaagacac aaattacaag aaacatctga
    34201 taatgaaaag gacataaaaa aacgtctgaa agagtttaag aaaactttag aacagaatga
    34261 ggttcttgat aaatttgacc gctatgtgtt tgaaagcatt gttgagaaag taattgtagg
    34321 tggacttgat gaaaatggaa atgttgaccc tgcacagttg acttttgttt ataaaacggg
    34381 cttgaagaat agcgtagatg gtgctaaatt taaaccacaa agaaaaaatg caagaggacg
    34441 acatagaact gacgaattgt gttcacatga cagcaacgag gttgataaaa tgtgttccga
    34501 tagtagtaac gacacattgt gagactgtag ttaaacttat tagagaataa cagggtttca
    34561 gacttttata ttgtgtctta aaagtagatt tgccacctat ttgccaccat aattattttt
    34621 gggtggcaaa tttattctac aatgatttca aaaatattta ccatctggtt tctcatgtta
    34681 ttagttacat gtaaatatgt atccatagtt gttgaagttt tagcatgtcc gagtatatgc
    34741 tggatatctt ttatattagt accattttct aataacatag tatcggggtt tttaaaataa
    34801 agtgtgtatt cttctaaagt agtgataatt aaaatatcaa atatcactat acaaaggaaa
    34861 gtgcacgctt tttatgttta acaaaaacga aatattaaaa aacttattga agaatatgat
    34921 gtcaaaacaa ctacagatat tcaagatatg ctaaaagatt tatttacaaa tacaattcat
    34981 caaatattta cattgtcgaa ttttaacggt ttaaacataa tatattgact gatataataa
    35041 aatcatagaa tattttaggg ggaaatagat tatgtttaag aaaaagttaa tagctgttgc
    35101 aatgagtata actatgatta gtgtggggag cttttcgtat gctcattcag gaagaactga
    35161 ttcatcagga ggtcatcgtg ataataaaaa taaatctgga ttaggaagtt atcactatca
    35221 ttgtggtggt tatccagctc atttacataa taatggtggt tgtccttata ctggaggagg
    35281 aagcagttct ggaacaacaa cttcggttaa taatgaagaa aagcaaaaga ggtctgttgg
    35341 agaaaaagga tataatcaag ggtatgaaga tgagtataaa ggaaattata gttcatcaaa
    35401 ttatagtgga gattatagtg atacatatga atcaaagtac tcagaaggat atgaaaaagg
    35461 taaggctaaa ttagaagagg aagaaaaggt ggctaaagag actggatata atttagggct
    35521 tacaggtgct aaatctaata atacatatga aaaagaagtt ttaaaaaatg cttatgatac
    35581 cggatattca actggataca atgaatataa gactaaaaag atagaagaat ataaagctaa
    35641 agggatagag gatagcaata aagataaaga aaaaatgaca tttgaagaaa acatagattc
    35701 tgaatttata gatgcttata ataatgctta tgacgaaata caagaacaat taaaaaatga
    35761 ttacactact caaggatttg agagtgctat caaaggagaa agatttgaca catcaactat
    35821 aggtaatgtt aaatatgcta attggtttaa agaaggatat gataatggta aggttaagtt
    35881 acctaaagca aaagaaagtg tatataatca aggttacaat gaagaagatt ttagtatacc
    35941 agatgaaatg aagtctattg aaactaagtt aaaaggtgta tatgatgaag gtttagaaaa
    36001 aagagaagaa gagaaaagta gaaatgctac ttatggggtt ggagctggaa cagcagctgt
    36061 agttgctgga gtagtatata agaagaaaaa acgtaaaaag atatagatgc tagatatacc
    36121 aatgatgaca agtgtataac aaatataatt ttaagtataa atatttaaaa tatagaaggg
    36181 aaggttatat ctatgggatt taactttagg aaaagcataa atctaggagg aggactcaaa
    36241 ttaaatttag gtaagaagag tgtaggtata agtgctggtg ttaaaggtgc tagagtgtca
    36301 gttaattcaa aaggaagaaa atcagctaca ttgagtatac ctggaactgg aatatcatat
    36361 acaaaaacta gtacaaagaa acgtaaacct aaataaatat ttgatatact aagaaataga
    36421 ctgttacaaa tagtctattt tttagtatat agatttaaaa agtgacaaaa ttttgaaata
    36481 taaactaatt tcataaagga aaatattctt ttatgtagaa ttataatatt taagtaatat
    36541 tataaaattt ataggaggat ttcattatga aattaagcaa aatactattg gcaataggga
    36601 tgtctgtttc attattgatt gctagttgtc caacatcaaa cgctcttagt tctattaatt
    36661 caataaaagg tacaaataag tatgaaacag caggtttaat agcagataaa caaaattatg
    36721 atattgcaat acttattaat tctgaaaatg gattatctga tggattgtca gcaagtggat
    36781 tgtcaggagc taaaaatgcc ccaatattgt taactaagaa agatacaatc ccttcagaaa
    36841 cattaaaaag attagaaaag gtaaaagaag tatatctaat tggaggaaaa aattcgatag
    36901 gagcatcagt tcagaaaata ttaagtgata aaaaaataag cataaataga ataagtggaa
    36961 ctgatagaat agatacaagt cataaagttg caaaagaaat aggaagagta agtacggtta
    37021 aaaaagtaat attaactaat gcttttaaag gcgaaccaga tgctatgagt gttgcttcta
    37081 tagcagttag agataaatct ccaatagtat taactgatgg aaatactaca agttttgtta
    37141 cagatggatt agaaacttat gcaattggtg gaaaagtcag tatgagtgat aagttaataa
    37201 atgaaactaa agcaacaaga ataggtggag tagatagata cgatacaaat aaacaaataa
    37261 taaataaatt ttataatggc tcaaaagaat tttacatagc aagtggaact gatttagttt
    37321 attctttagt tggttctaca attgcacaaa aaacacctat agtgttagtt ggaaatggaa
    37381 gtaataagag tatattaaaa aatgcaagta aaataactgc tattggaaat attgatgatg
    37441 ttgttataaa acaatgttta aatataacta ataatgttgg ggattcaaat acaggtattg
    37501 taaaagatac taattcaaat aatacgaaca aaccaagtaa tgagcctagc tctaataatc
    37561 ctggattaac tgataaaagt acagtatatg taactcctaa tggaaaatca tatcatctaa
    37621 caaaaaattg ttctacacta aaaagaagta aaacaataaa taaagtaaca ttaaaacaag
    37681 ctaagtcaca aggtaaaata gacccttgta atgtttgtgt aaaataatgt attagccaaa
    37741 tatatattaa aaaagacaat taattaagat tgtcttcttt attgaagaaa tataaattta
    37801 tattatgtat tattaaaaaa aaattattaa ttataaaaaa taataataaa gacgataagt
    37861 attaaaattt tatgtttcca atatttatga tataattaat atggcaagga ataaatattt
    37921 gaaaaagcta tgagtggtgt ttccatggaa gtttcttcac tttcttatga gaggaggtga
    37981 aaactatgga aaatctaatg atgagtatta taactggtgt tatagacagt tacatttacg
    38041 ataaattaaa gagccactct gagcgaccaa ctaagagtgg ctgggaactt aatataaagt
    38101 tccagaaaag taaatcttca aaataatttg ttgaaggaaa catcactcaa agttaagtaa
    38161 aatatgtttc cttgctttta ttatatcaca aactaaaaat attaacacaa aattttgtga
    38221 atatatactt attattgttc taaaaaaata tgtaaataat tgatttgatt tcatagaatt
    38281 attgttgtaa tgtttagtta aatatacaca atctcaatta aagaagataa gtactataaa
    38341 ttcatatcaa aaaaatacat aaacttttaa gttataaatt tataaaaagt cttttagtat
    38401 cattatttca agaggaatta acatatgaaa aaataacttc attagaaaag tggataaaaa
    38461 actggtaaga agatgactaa agattacata tactattact taaattaaaa atttaatata
    38521 atttataatg tatacaattt attataaaaa atttaatttt aattttctat ttttgatact
    38581 ataatatata atcttgataa tttaattaaa aatctagatt ttacatacca aactggtttc
    38641 ttatttcgct atctttactt atgaactcat atattcctat aaaggttatt ttagaaaaag
    38701 ttggttattt aaacattaat accacaattt ctatagattg tttgttgaat aatctataat
    38761 tatattatgg actaaaaaat ccaatgagct aatttattgt ttttttatat ttgagagatt
    38821 acaacttatt agtttgtata tctctttttt taataaaatc aataatatat aaaactatt
//
LOCUS       DAEMHZ010000021        38847 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000021 DAEMHZ010000000
VERSION     DAEMHZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.021"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..461
                     /locus_tag="KPK72_001004"
     CDS             <1..461
                     /locus_tag="KPK72_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2488244.1"
                     /translation="LKENHITFEEYDHEPILSFEKAKEIREKLQYTGVESKSLFIKDR
                     SGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPFGYEKNISL
                     VIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF"
     gene            complement(1160..2314)
                     /gene="nagA"
                     /locus_tag="KPK72_001005"
     CDS             complement(1160..2314)
                     /gene="nagA"
                     /locus_tag="KPK72_001005"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2488245.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFETINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(2397..3122)
                     /locus_tag="KPK72_001006"
     CDS             complement(2397..3122)
                     /locus_tag="KPK72_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2488246.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITTGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3391..4320
                     /gene="pfkB"
                     /locus_tag="KPK72_001007"
     CDS             3391..4320
                     /gene="pfkB"
                     /locus_tag="KPK72_001007"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2488247.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            complement(4449..5366)
                     /locus_tag="KPK72_001008"
     CDS             complement(4449..5366)
                     /locus_tag="KPK72_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2488248.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            5837..7006
                     /locus_tag="KPK72_001009"
     CDS             5837..7006
                     /locus_tag="KPK72_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2488249.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            7055..7906
                     /locus_tag="KPK72_001010"
     CDS             7055..7906
                     /locus_tag="KPK72_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2488250.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQLVKDFVKEKIKV
                     CGSVNRV"
     gene            8232..10133
                     /locus_tag="KPK72_001011"
     CDS             8232..10133
                     /locus_tag="KPK72_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2488251.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            10136..11179
                     /gene="ypdE"
                     /locus_tag="KPK72_001012"
     CDS             10136..11179
                     /gene="ypdE"
                     /locus_tag="KPK72_001012"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2488252.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVIEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            11194..11499
                     /locus_tag="KPK72_001013"
     CDS             11194..11499
                     /locus_tag="KPK72_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2488253.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            11596..11931
                     /locus_tag="KPK72_001014"
     CDS             11596..11931
                     /locus_tag="KPK72_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2488254.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            11963..13285
                     /locus_tag="KPK72_001015"
     CDS             11963..13285
                     /locus_tag="KPK72_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2488255.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            13295..14365
                     /locus_tag="KPK72_001016"
     CDS             13295..14365
                     /locus_tag="KPK72_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2488256.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            14370..15557
                     /locus_tag="KPK72_001017"
     CDS             14370..15557
                     /locus_tag="KPK72_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2488257.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(15754..16626)
                     /locus_tag="KPK72_001018"
     CDS             complement(15754..16626)
                     /locus_tag="KPK72_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG2488258.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNVLWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(17020..17211)
                     /locus_tag="KPK72_001019"
     CDS             complement(17020..17211)
                     /locus_tag="KPK72_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488259.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            17431..18477
                     /gene="cobT"
                     /locus_tag="KPK72_001020"
     CDS             17431..18477
                     /gene="cobT"
                     /locus_tag="KPK72_001020"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG2488260.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            18509..19069
                     /gene="cobU"
                     /locus_tag="KPK72_001021"
     CDS             18509..19069
                     /gene="cobU"
                     /locus_tag="KPK72_001021"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG2488261.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            19079..19852
                     /gene="cobS"
                     /locus_tag="KPK72_001022"
     CDS             19079..19852
                     /gene="cobS"
                     /locus_tag="KPK72_001022"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG2488262.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            19872..20486
                     /locus_tag="KPK72_001023"
     CDS             19872..20486
                     /locus_tag="KPK72_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2488263.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKVKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      20553..20732
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20862..22361
                     /locus_tag="KPK72_001024"
     CDS             20862..22361
                     /locus_tag="KPK72_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG2488264.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            22400..23776
                     /locus_tag="KPK72_001025"
     CDS             22400..23776
                     /locus_tag="KPK72_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG2488265.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALIKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            23777..24748
                     /locus_tag="KPK72_001026"
     CDS             23777..24748
                     /locus_tag="KPK72_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2488266.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYVIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            24780..25850
                     /locus_tag="KPK72_001027"
     CDS             24780..25850
                     /locus_tag="KPK72_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2488267.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            25852..26739
                     /locus_tag="KPK72_001028"
     CDS             25852..26739
                     /locus_tag="KPK72_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2488268.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            26727..27359
                     /locus_tag="KPK72_001029"
     CDS             26727..27359
                     /locus_tag="KPK72_001029"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG2488269.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            27368..28594
                     /locus_tag="KPK72_001030"
     CDS             27368..28594
                     /locus_tag="KPK72_001030"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG2488270.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            28591..29199
                     /locus_tag="KPK72_001031"
     CDS             28591..29199
                     /locus_tag="KPK72_001031"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG2488271.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            29189..29764
                     /locus_tag="KPK72_001032"
     CDS             29189..29764
                     /locus_tag="KPK72_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG2488272.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            29757..30509
                     /locus_tag="KPK72_001033"
     CDS             29757..30509
                     /locus_tag="KPK72_001033"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG2488273.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            30493..31614
                     /gene="cbiG"
                     /locus_tag="KPK72_001034"
     CDS             30493..31614
                     /gene="cbiG"
                     /locus_tag="KPK72_001034"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG2488274.1"
                     /translation="MNIEVNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVQFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            31611..32336
                     /gene="cobJ"
                     /locus_tag="KPK72_001035"
     CDS             31611..32336
                     /gene="cobJ"
                     /locus_tag="KPK72_001035"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG2488275.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            32333..33085
                     /gene="cobK"
                     /locus_tag="KPK72_001036"
     CDS             32333..33085
                     /gene="cobK"
                     /locus_tag="KPK72_001036"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG2488276.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            33104..34582
                     /locus_tag="KPK72_001037"
     CDS             33104..34582
                     /locus_tag="KPK72_001037"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG2488277.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            34612..35517
                     /gene="hemC"
                     /locus_tag="KPK72_001038"
     CDS             34612..35517
                     /gene="hemC"
                     /locus_tag="KPK72_001038"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG2488278.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            35507..37006
                     /gene="cobA"
                     /locus_tag="KPK72_001039"
     CDS             35507..37006
                     /gene="cobA"
                     /locus_tag="KPK72_001039"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG2488279.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKMYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            37023..37988
                     /gene="hemB"
                     /locus_tag="KPK72_001040"
     CDS             37023..37988
                     /gene="hemB"
                     /locus_tag="KPK72_001040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG2488280.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            38141..38719
                     /locus_tag="KPK72_001041"
     CDS             38141..38719
                     /locus_tag="KPK72_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG2488281.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEKRYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        15510 a         4079 c         6844 g        12414 t
ORIGIN      
        1 ttttaaaaga aaatcatata acgtttgaag aatatgacca tgaacctatt ttatcatttg
       61 aaaaagctaa agaaataaga gaaaaattac aatatactgg agtagaaagt aaaagtttat
      121 ttataaaaga taggtctgga aattactata tatttgtaac cacagaagga aaaaaattag
      181 atccaaaaac tataaaaaat gttttaggtg gaaaagtatc tatatgttca aaagaagaat
      241 taacacaaaa aacacaatgt ttgcctggat gtgtatctcc ttttggatat gagaaaaata
      301 tctctcttgt aatagatcaa gatatattta accatgataa aataatcttt tcaccaggga
      361 taccagaaaa gacatttatt attacagcta gtggatttaa aaaagtaata aacaatataa
      421 acaatacaac ttataaatat atatatatta atgaatttta atcataattg taaattagag
      481 aaaatagcat tagaaatcat gcttaactat agtgtaaata aattatattc aacacaaaag
      541 attgatgcta ttattacaat agttactgtt aagctcaaat tcaatgtatt atgagttatt
      601 atttttaaat tttaattaca aaagcagggt agttttaact actctgcttt atatttaaca
      661 taaaatatat aaaatcaaca aaatgatttt agtctatcta ttagatattc ttcgctctca
      721 atgcttagaa cttttagata tatacaataa gtgctttcat ttgtaaaaat ttctatagta
      781 ttatcttgtt tatttacacc tacaatatct ttaaaattga tatgattttt agtattttta
      841 catttgaaaa tatatctgtt atcaatcaaa atctcatcat tagtaattga aatatagggt
      901 ttatctcatg ctttattaaa aatttatatg tttgctcaag ataaaaactt acaaataata
      961 atagaaaagt tactttaaat gtacatatta tcgaaaatat tatcacaaaa atacttataa
     1021 tatatatttg tttaaaataa cctctatcgc aatagtttat taacgatatg gaaataaaag
     1081 tgttaaatca gattttggag aaataacttt aaatatttcc agactctttt tagacagcct
     1141 ccataacctt atattgaacc tatttattaa ctttgtactt acagatacca tttatataag
     1201 tttcgtgtat attaaagtca ttatctgtta ctataaagtc tgcatccata ccttgatata
     1261 tagagccaca gacattatca atattacaac ttaaagcagg gacatatgta gccattttta
     1321 ttgcatcttc taaagttgat atgttccaat caactatatt tctcacagcc ttgtccatag
     1381 ttaatacact tccagctaaa ctcccactat ttaatcttgc aacactattt tttacattta
     1441 cattaaactt accaagagta tattttccat cctccatagc accagctctc atacaatctg
     1501 taattaaagc tatgttatta ctagacttag catccatcat tattttaaca gctattgggc
     1561 ttacatgatg accatcacaa attaattcag cataagtatc ttttagtacc atagcagccc
     1621 caaccattcc aggttctcta tgatttaaag gactcatccc attatatgta tgtacaaaca
     1681 ctttggcacc tgaattaacg acttgtttaa cttgctcaaa tgtagctgaa ctatgaccaa
     1741 gagaaactga tatattattt ttagttgcat atcttgttaa ttctattgaa cctttacgtt
     1801 ctggagcaat tgctattttt cttattaaat ttttcgattt tttttgccat aattttaata
     1861 tattgatgtc agggtcttta aaatattttt cattttgagc gcccttatat ttttctgtaa
     1921 aaaatggacc ttctaaataa atcccttgta tttttgcacc ctcaactcga ttgaagctat
     1981 tgcttatatt ttctacagaa tcacttagtg tatctacaga atcagttaaa gttgtaggta
     2041 ggaaagatgt tactccatat tttaataagc cttttgaaat tgtgtttatt gtttcaaatg
     2101 tattgtccat gacatctgca cctaaaagac cgtgaatgtg agtatcaact aagccaggag
     2161 caatatattt acctgaaaaa tctataacat tatcagtaat attaggtctt tcttgttgaa
     2221 attcaccgaa tttgccatta attatactaa ggtaaccttt ttctttgatt ccatgtttca
     2281 taaaaaattt atctgcataa ataaaacttt tcaaaataac ctccttataa ttatactatc
     2341 attgtggcat tgacgtttat aatatcattc tattttcttg aaagctattt aacttactat
     2401 cttacatgtc ttactctata tacaaattta tcgcttctag cgacactcaa agtaaattca
     2461 ataactatat tgtcatcatt atatgtggta cgaagtaatt tcagacaagg tgaatctcct
     2521 ggtacttcca ataacttagc ttccttatca gagattattc cagctgaaaa ttcttcatca
     2581 gcaactttaa taatctcgcc ataatcttct ctaaatattt catataaagg tttttgttcg
     2641 attttttctt ttgtcaatcc tataaattct ttcataggaa gatatgttct ttcaagcatc
     2701 ataggaaggt catcagcaag tcttaaacgc attattttta tcattttttc accagtagtt
     2761 atacctaatt tttcagagaa atactttgta ctttctacta cctcaaatga taataccttt
     2821 gtacctggct tttttccttg tgttctcata taatctgtaa aactataact ttccattaag
     2881 ttttgagaat tttgacttag tgcagcaaca aaagttcctt ttccatgtct tttatatata
     2941 tatcccatat gctctaactc agccatagct agtctaactg tggttctact tactccatat
     3001 tcatcacaaa tttttctttc ggaaagcatt ctatcgtttg gttccatctc attttcaatc
     3061 ttagttctta atatctcaac aagctggtca tatattggtc ttttatcatt tctttttatc
     3121 ataaaaatat agtcctttct aagtggttat taccacaatt tgattataga atatagaatt
     3181 atttatgtca atgaataaag tttaatcaaa attcaataag taaggctatt aatttattat
     3241 aaaattatgg tacattgaca aaacaagtaa aataatttat attcaaattg gtaataacca
     3301 ctttggatat attatagatg aggtattttt tattttacag attaatttat tgtttagaaa
     3361 agtaaaaatt aataatgagg agaatctagt atgattacag taataacatt caacccatct
     3421 atagatagaa tgtatagagt gaataatata aacataggtg aggtgcaaag ggtagtaagt
     3481 aagaatgcaa ctgctggagg taaaggatta aacgtagcta aggtatgtaa aattttaaaa
     3541 gaaaatcctt tagctatggg atttctaggc ggatttaatg gagaatttat aaaaaatgaa
     3601 cttagaaaac tagatataca aaataaattt acaaaaatag agcaggaaac tagaaattgc
     3661 ttaaacataa ttgaggacaa taaagttagt acagaatttt tagaaaaagg accaatagta
     3721 gaaaaaagcg atttagagag atttgaaaat gatatagagg aagtactaaa aaatactaaa
     3781 atactggtag cttctggtag ttattgtgag aatatgccga tagactatta tgaaaagata
     3841 ggaaatatat gtagagcaaa taatataaag tttatattag atacatcagg agaagcatta
     3901 aaaatagctt taaaatcaaa accatattta ataaaaccaa atatagatga aataagacat
     3961 ttattaggaa tgaatataga atcaagagag gaaattatct tgtcaggaaa aaaacttatt
     4021 gaaatgggtg cagagaatgt atgtatatct ttaggaaaag atggtatggt gtatttaaac
     4081 gaagataatg tatatgatgt aaaagtacca gttgtaaagt gtataaatac tgtaggtagt
     4141 ggagatagca cagtggctgg atttagtgta ggtattttaa ggggttataa aatagaaaat
     4201 ttacttaaat ttgcaaatgc ttgtggtata agtaatgctt taaatatgga aactggattt
     4261 gtagatttgg tagaaattga taaatatcaa gatttagttg aagtaaaaaa gttaagttaa
     4321 atacatttaa tgggaatata ataaatttaa tgttgttgta atagatttta attttaatat
     4381 aaaagatata gtcttatata aaaataggtt agaaaaattc atttttaacc tatttttata
     4441 ataaatatct attttactga atttataatt ttctctagta atctttgagc cttgatacac
     4501 tcttctccat taatagctgg ttttatatta ttttctatac aatcaataaa atgatttgat
     4561 atatcctcaa aacccttttg ttttaatatg tttacccatg caccactttg ctttaaagtt
     4621 aggtttccac cttcttctat ttccagtaca ttaagattct taactctttg aatcttaccc
     4681 tttgaaagaa tttcaacttg ttctaacttt gttccagaat ctctatgcat agaggtgttt
     4741 atagagaaat ttttaccttt aagtttatga cttacaaata taagattctt attgtctgtt
     4801 aaaaataaat cctctccact aatttcaaca tcttcatttg ctaaccataa agcagtgtca
     4861 ataacatgta tatagtcatc tataagagta ctgtcgaatt taacatttct taaggaattt
     4921 aatccatgtt tacatatatt gatagataca atttctgtag cattattttt aatttctttg
     4981 tacataggac agaatctacg gttgaatcca accattaaat ttaagttttt tttcgtactt
     5041 aactcaataa gttcttctcc ttgacttact gttgaagcaa gaggtttgtc tacatataca
     5101 tgaacaccta aatttaataa tatttttata atttcgtagt gtgtttctgt agagctatgt
     5161 agaaaaatac aatcacattt tttagctagg gattcaatag aatcgaatgg catgattcta
     5221 taatcactac agactttttc ccttttaact ttatttggag taaaagctcc tacaaactca
     5281 aatctctcag acttagttaa tataggtaaa tatgcctttt gagctatgga gccaagacct
     5341 atcattccca tttttatatt tttcataata ctttctccta aggtttttat atttacagaa
     5401 ttgattataa ttcagacaca taaatttgta aactttgtat caaaaaataa atttaaagct
     5461 tatataagta aattagcgaa ataagaaata ttgataaagt aattttgtag gatatctata
     5521 aaattgattg atataaatat tttatgtatt gacaaaaaat tcaaattaat ttatatttgg
     5581 attagtggta attaccactt tggtctgaac aagtttttat cgtaaaataa aaatactaaa
     5641 gtatatagag tatgatttta ttaaattttt aaagataaac tataaaatta attaaaatct
     5701 atagcgtata ttttatagtg agtttaagta gtactaaaaa atctaagcaa aaaattataa
     5761 aagagtatat ttttttatag tttaaagtgg ttataacctc attaccaaat gtaaacgtta
     5821 caagtatgaa aggagaatga aaatgtttaa attagaagaa caaaaactaa aagatttagg
     5881 tgcaataata acaactaatg aaataaaaca acagcctgaa ttatggttag aaacttatga
     5941 aatatataaa tctaacaaag aaaaattaag tagattcata gatacaataa gtaataatca
     6001 tgggcagttt agagttatat ttacaggtgc tggaacttca gcttacatag gaaattcaat
     6061 acttccttac cttaaaaata aaaatgacat aagaaaatac atatttgaag caatacctac
     6121 tacggatata gtttcaaacc catatgatta cttaaaaaaa gatataccaa cattattaat
     6181 ttcatttgca agaagtggaa atagtcctga aagtttggct gctttaaatt tagggaataa
     6241 gattgtagat aatttttatc atttagctat aacttgtaat ccagaaggag aactggctaa
     6301 gatgactaaa aacgacgaaa ataattactt gttattaatg ccatcaaaat caaatgatga
     6361 aggctttgcc atgacaggaa gtttttcgtg tatgatgtta tctgctatgt taatattcga
     6421 tagtttagaa gatgatgtag aaaagtcata tataaatgca ataattgaaa tgggaagaaa
     6481 tgttatagat agaaaagatg aaatacatga gttaataaat aaagattttg atagagttgt
     6541 atatttaggt tcaggaggat taggtggttt gacacaagaa gcacaactaa aacttttaga
     6601 acttacagca ggaaaaatat ctacagtgta tgattctcct atggggttta gacacggacc
     6661 aaaatcattt attgatgaaa atacacttgt atttgaattt gtatctaact gtttatatac
     6721 aagaaaatat gatttagatg ttttagagga gataaaaagg gataaaattg ctaaattcac
     6781 ttgtgcagtg tcagtagaaa atgaaaataa cttctcagga acaaagtttg aatttaaaga
     6841 aaagtataat aaattaccag atgtgtatct tgcaatgcca tacatattgt ttgcacaaac
     6901 tatagcattg tttgtatcgg taaaggttgg aaataagcca gatactccat cagctacagg
     6961 aacagtaaat cgagtagtaa aaggtgtaac aatatatgaa tattaaaaga taagattaaa
     7021 atattataac taaataattt gggagggtat caatgtggca ttagtaacaa caacatcaat
     7081 gcttaaaaag gcacaagatg aagggtatgc agtaggagca tttaatgtag aaaatatgga
     7141 aatggtaatt gctgttatag aagctgctga ggagttaaat tctccagtaa tattacaaac
     7201 tactccatct actgtaaaat atgcaggtct tgattattat ctagcaaatg caaaggttgc
     7261 tgcagaaaga gtaaatatac ctgtagcaat gcatctggac catggttcaa gttttgacct
     7321 tgcaatgaaa gctttaagaa gtggatatac atcaataatg atagatggtt ctcatgaaag
     7381 ctttgaaaat aatatatcta tatctaaatc agtagtaaaa gcatgtagtc cttctcttat
     7441 accagtggaa gcagaacttg ggaaagttgg tggaaaagaa gatgatttag atggcgggaa
     7501 tagtaatgat tatacaaatc caaaagaggc aaaagagttt gtcgatagaa caggaatatc
     7561 ttcattggct attgctatag gtactgcaca tggattgtat aagggtgaac ctaaaataga
     7621 tttagatagg ttatcagaaa ttaaaaatgt agtttcaata cctttagtat tgcatggagg
     7681 ttcaggaata catgatgata ttataaaaga agctataaaa agaggtattg caaaagttaa
     7741 ctatgcaact gaattaagaa ttgcatatag taaaggggtt aaaaaagttt tagatgaaga
     7801 ttctgaggtt atagacccta aaaagtatgg attagcaggg cttcaattag taaaggactt
     7861 tgtgaaagaa aagattaaag tgtgtgggtc tgtaaataga gtataaaaat aaaatatcct
     7921 aaatttattt tagataagtt taggatattt gttatatgaa aaatttatat taattatatg
     7981 taaatggtgt atttttttca tttaattgat gaaaaatatc agcttttatt ggtaaagtat
     8041 tacatatata attaaaatat actaaaaatt gaactatata ttttattatt ataaaatatt
     8101 tatattaaat tgtggaatta aaatatacta ggatataaaa ttcttaaata aaaagaaaag
     8161 gttgttttta taaaaaatat tttttatacc actttaaaaa acacaataat aacactgagg
     8221 gaggtgggga tatgagagaa cgcattattc ttgaaacatt aaaaaatagt agtggttatc
     8281 taaacataga ttattttgca gataagttgg gggtatctac tcgaactatc agaaatgaaa
     8341 taaaaaagat agaaactata aaagaatgta atggttttaa attagaatat aagtctaaat
     8401 taggatatat tctaaatatt aaagaccaag ataaattcga gcattatcta agaaacttac
     8461 cctcagattt agttgaaaat ccagaacaac ggttggagtc tataatagta gagcttttag
     8521 taaatgaagg ttataaaact atagaacagt taagtaaaaa atttctagta agttcatccc
     8581 aaataaaaaa tgatttaaaa aagatagatg aaaagataaa ggatacagaa ttaaaactag
     8641 agcgaaaggc tcattatgga attaaaatag aaggttctat aaaagaaatt caaagtatat
     8701 tggtggatag ttattttaga gggaatagaa atataacaga gtacaggaat aagtttatag
     8761 ataatttaaa attagctaat attagaagta caataaaaaa tgtacttaat gaacatgatt
     8821 tagaagcaaa tttaacagaa cttgaggaaa tattagcaca aattattata ttgtatataa
     8881 gagttagtat gagagtagcg ggaaatgcta gcgatttaaa attatataca gaagatttaa
     8941 ttatagataa attattagat aaaattttta gagataaaaa atataacctt aattatgatg
     9001 aaaagtatta tttaaaggag cttataaaac taaagactaa agataagaaa gccactataa
     9061 agaatattga taagaataaa cttcaagata ttatgttcga attttttaga gagatagata
     9121 aaaaatataa ctctaatttt ttagaagata aagagttttt taacttattt tacttacata
     9181 ttgcatgttt aatagaacgt ataaagaaga accacaagat aataaatcct ttttcagtta
     9241 aaataagtca acagtatcca actgttttta atttatctat tcaattttca aaaattatag
     9301 aaaatgagta tcatataaaa ataagtcaag atgaaatagg gtttatagct actcacattg
     9361 ctgttccttt tgaaaagaga gaagaagcta attttaataa aaaatataaa atagcaataa
     9421 tatgctcttc tggtggaggt agtgcatttt tgataaattt aagacttaaa gaaatttttc
     9481 caaatgctga gataagaaat ttttcattac tagaagaaaa agctgtaata gaatttgcac
     9541 cagatttgat tttttcaata acaaatcttt tatttgaaat aaatgctcca gttatactta
     9601 taaatgaaat tttagacgaa ttggattatt tagatattaa agaaagtgtt agatttgcag
     9661 accatatagg aaatatatca agcccaaaac aatatatatt aggtctattt gataagaatc
     9721 actttaggtg tataaaagat aaagctaagt ataaggatat ccttgatagt atgtcaaaat
     9781 gtatagttga cgagggagct tgttctccaa cctatcccaa agatgtatgg gaaagagaaa
     9841 gctatttgag tactatttat acaaatgggg tggctatacc tcatccaatc gagatgacag
     9901 gaaacaaaaa tataatatca gtagctttga tacaaactga tattatttat gaaaataggg
     9961 tgcccaaaat tattttcatg atttcattaa taaagggaaa tttagaactt cataagcaga
    10021 tatctaaata tttaagtaag attatggcaa ataaggatat ggtagacatg ctaaataagt
    10081 cacaatctta tgaagagttt atgtataagt taaaaatcta tctgggaggt tagacatgaa
    10141 tatagagttt tttaagaggt tgtcagaagc agatggtata gcatctaatg aagaagaggt
    10201 tagacatgtt ttattggaag aactaaaagc ttatagtgat aaaattatct gtgatgggct
    10261 agggagtatc atcttttcta aaataaaaga tgagagtgca ccaaacgtaa tgatttgtgc
    10321 tcatatggat gaagttggat ttatggtgag aagtatagac aaacttggta tgattcactt
    10381 aataacaata ggaggagtaa aacctcttgc acagtttgta caaaaagtta gaattacaac
    10441 aaaagaaggg aaaaaaatcc ccgctgtcat aaatgctaca tataataatg gtaaggctga
    10501 aaatatatat gcagatattg gagcatatac agaagaagat gtatataacc tagggattaa
    10561 tgttggggat atggtaacat atacaacaag ctttgaggaa tttactttac cagaccgatt
    10621 agtgggaaag gcttttgatg ataggatagg atgttttgtg atgggagaag ttttaaaaga
    10681 acttaggaaa gaaaatctaa attgtaatat tcactttgcg gctacaagta gtgaagaggt
    10741 tggaataagg ggagcaaaga catcaacaca gttaataaat ccagatatag tatttgtaat
    10801 agatgtagca tgtgctaaaa atgagtttgt gagagactat acaaatcaga aacaaattga
    10861 taaaggtgta atgctaatgc atagagatag gactctagtc cctagtagaa aaatgataga
    10921 ttatgtaata gaaattgctg ataaaaataa tataccactt caacatgata tgtttgaatc
    10981 aggtgggact gatggaggag aagcacatct cgtaaatgaa gggaaacctt gtgtagtaac
    11041 ttgtgtacca gttagatatg gtcattgtgc attttcaata gtaagtaata aagatttgga
    11101 gaacattata agattgtata cacagttaat tttaaatttt gatgaagaac aatataaaca
    11161 tataagaaat tttatctaaa ggagaataag aaaatgtcaa cagaagtaca aataatggaa
    11221 cttatatcaa ctgcaggaga gagtaaatcg aaagcttttg aggctttaaa aaaagtgaag
    11281 tcaaaagatt ttaaaggagc aaaattgtta atagaggagg ctcgtaaaat agatattgaa
    11341 gctcacagga tacaaacaga attaataact caagatttaa atgaagatgt tgaaagtcca
    11401 gcaatgagtc ttttaatggt acatgctcaa gaccactata tgacttctca gctagcaaaa
    11461 gacttgatag aggctttgat atattgtctc gataagtaat tgtaatgtag aaatagatta
    11521 tttatgtaaa aattctttta aaagtttaga tacttttaag agataataaa aatacaaact
    11581 ttaggaggaa ttattatgaa aatattatta tgttgtgcag gtggattttc tactaatatg
    11641 ttaatgcaaa atatgaaaaa ggtggtaaaa gagagtgcta aactcaatga aaaagatttt
    11701 gagtttacag ctataccagc agattcaata gaaagtgaag ttgatgattg ggatatagta
    11761 cttataggtc ctcaaatagc tcacaaaata gacttcttaa aaggaatatt agagccaaaa
    11821 aataagcctt ttgcagtaat agataaagat gtatatggaa aaatggatgg agctacagtt
    11881 atgaagttgg cattaataac ttataagaaa catcaattag ctcaaggata ataaaaataa
    11941 tgagggaaaa gggagattag atatgttttc aaaatttgaa gcatttatga atagagtatt
    12001 tatgccttta gctcataagg ttgacaatca acgtcacctt ggttctataa aggctggtat
    12061 ggttgctatg acaccattca caatattagg aagtatattt gcaatacttc cagcacttcc
    12121 aaatatgctt ggagaaaata atccaataag caaattcata cttaacaatt cagcaatatt
    12181 ggatttacca gtaagtcttt ccataggttt aataggatta tatgcttgta tgtgtattgc
    12241 ttttaatctg gggaactact ataaattata tataccaggt tgtgttgcac tatcaacttt
    12301 ttctttttta ttcctagtag cctcatttac accagatgga gctttagaaa taggaaattt
    12361 aggagctaaa gggctattta caggtatgat agcagggctt ggttctgtgg agttatataa
    12421 tttttgtaat aagaagaata tcacaataaa aatgccagaa aatgtacctg attttgtatc
    12481 taggtctttt gaattaatac ctactactat aatagtttgt ggaactttta tagttgctcg
    12541 ttttatttca ttaaaagtat ttggagaatt acctccacag atacttactc aatttttatc
    12601 tccattagta ggaagtatgg ataacccatg gtcagttctt gcacttaact ttgctatatg
    12661 tactatattc ttttttggaa tacattcatc agtatttagt cctataacta gacctataat
    12721 gattacattt atagctgaaa acatagcagc tatgcaagct ggagaaacaa tacctcattt
    12781 ctttacagca ggagcttcaa gtgcattctt tggatttaca ggatgtggaa ttagtatagg
    12841 ttgtgtaatt gcatgtatgt tatcaaaaaa tgcttcctat aagaagattg gacgtgtatc
    12901 attattccct gcattatttg ggataaatga accaatatta tttggagcac cgataatatt
    12961 aaatccaata atgtttatac cacatgtatt tggtggagct ataataggta cactaccaat
    13021 gttttttatg cactggggat ggttagcaaa gccagtattt gaccctccat atgtaggggt
    13081 attcttagaa ggatttttaa ctaatggaga ttggcattct atattagcaa atgcattgca
    13141 attagtatta gcagttttat tatattggcc attctttaaa ataatggaac gtcaacaaaa
    13201 taataaaaaa caaaaagaag aaactaagaa tatattctct aaagaagaag aagacttatt
    13261 ggatgattta ggattggatt tctaatattt tgttatgaat aatacaatgg agagattagt
    13321 atccttaatc tataaaagag gtgttgacgc tcttttgatt aaagcaaaaa atactaaaag
    13381 atatatagga gctttatcag gtagtggtgt atatgtgctt gttactaagg ataaaaatta
    13441 tcaaattcta gatggcagat atattgatga agcagataaa aaaacgactg gtttcataaa
    13501 aaggattgta tctcaaggtt cttatatacc aactataatt gagttattaa aagaattaaa
    13561 tataaaaaag ttagcaatag aaaaccaagc tatgagtata caagagtatc tttcactaaa
    13621 aaatgaaggt tttgaaatca atttaataac aaatgaatta tggaagctaa gggctataaa
    13681 atctaaagaa gaaattgaac ttattaaaaa ggcttgtgag attacagatg aagtatttga
    13741 tgaggttatt tctgaaatta aaaaagatat gactgagcta gaagtatcag ctctaattca
    13801 gtatcatgca ttaaaaaaag gtgcaagtgg aatgtctttt gaaacaatag tggtatcagg
    13861 agaaagaggt gcaatgccac atggaagacc tacaaacaaa aaattgaaag tgaatgaagc
    13921 tataacaata gattttggtg ttgtttatca agggtatcaa tcagacatga ctcgtacaat
    13981 atctataggt aaacctccta aaattattaa agagatatat gatgtagtat tggaagcaca
    14041 attatctgca atagagtcaa taaaggaagg aactagggca agtgatgttg acaaagtggc
    14101 tagaaaaatt atagataaac atggatttgg cgaatatttt aatcatggac tgggtcatgg
    14161 tataggtctt ggagatggtg aagttcccac tttaaatcca aatagtgaag atatattagt
    14221 ggaaggaatg gtaatgtctt gtgagccagg tatttatatt ccaaatgtag ggggagttag
    14281 aatagaagat gatattgtta taatagatgg aaaaggtgtt ccactcaata agacatccaa
    14341 agagtttata atattaggag agtaaaatta tgaaatataa ttttaataaa gtattaaatc
    14401 gtaaagaagg aaattgtaga aaatggagca accatgtgat taaagaaaaa tttggcttaa
    14461 atgaagatgc aattcctatg gatttagctg atatcgattt tgagtgtgct cctgctataa
    14521 aagaatctat cttaaaaaga gcatcagtag gagattatag ttatactttt ataggagatg
    14581 acttctatga tgctattata aattggaata aaagaagatt tgatgtatat atagaaaagg
    14641 attggataaa attaactttt ggtactgttt caacccttca ttatatagtc caagcatata
    14701 ctaaagaagg agagggtgta ttgataaata cacctgctta tgaccctttt gcagaggctg
    14761 tagtaaataa taatagaaag ttgtgttgta gctcattaaa attagataac aatagatatt
    14821 atttagattt agaggatatg gaaaatcaaa tgaaatacga gaatataaga ttgcttatat
    14881 tctgtagtcc acaaaatcca tcaggtcgta tatggacaaa agaagaacta tatcaagtgt
    14941 cagagttatg tttaaagtat aatgtaattt tggtaagtga tgaaattcat agagatataa
    15001 tatttaaaga ttatagattt gtatctttat ggaatgctca tccagaaatt tataaaaata
    15061 gtataatctg tgtatctcct aataaaggct tcaacttagg tggattaaaa acatcatatg
    15121 tattaataag agatgtaaaa attagggaga ctttattaga aaggctaaaa agtaattcaa
    15181 taacttctcc aaatgtattt gcaataccag caatagttgc agcatacaat gaaagcgaag
    15241 aatggctaga tgctatgact agttatgtag aggagaattt tgaaatagtt tatgatttct
    15301 ttgaaactaa tataccaaaa gctaaagtga tgaagtcaga ctcttctttt ttagcctgga
    15361 tagatgtaag agaagtattt agaaatgaag aagaatctaa agaatttttt agacatgcaa
    15421 acttaactat ggtagttgga agttattttg tgaaagatgg agatggattt attcgtatta
    15481 atgtggggtg tcctagagaa acattaaatg aggctctaaa tagaatcaaa aaaacgtata
    15541 taagtatgta ttgttaaatc ataaaaaaag tatctatggt ttatataaaa acatattatt
    15601 tagttatcaa atattactgg aattttgtat tccagtaata ttttttgatt aaggaatatt
    15661 attgaggaat attaaataat attatttcat acccttatat taacttatat aaaaatattt
    15721 tattattttg ggaacgggtt agctaacctt tatctagtgt atatttatat tgtaagaagc
    15781 agtaaaaact tttcctttac taagtttatt aataaacttc ttatctttat aaattttgtt
    15841 agaatttata ctatcagcaa gaccatacca aggttctata cataaaaaag gagcagtaga
    15901 attagtttca ctataataag gagtccaaat accaactaga gggaaatctt ccatagatat
    15961 agttacatat ttactaccat cattattaca tatagacact tcatctatgt tggtgtatat
    16021 caaagcatca ttcttgaata cttctggatt taactgtaag ttttttaatt ttccaatagg
    16081 tattatatca tctgtaaaag aaccatttaa attgattttt tctacatcat ttttactttt
    16141 aaactcaagg tagtgtttag aaaaatcatt ttgttcgtaa aaaggaaggt tgaatgcagg
    16201 atgtccacct atagaaaaga atatatcttt agaatcagta ttttttacaa tccattctac
    16261 ctcaatacta ttatcattta atgtatagtt tatgattagc tcaaaagaat atggatactt
    16321 ctttaaagta gattcattat ctgttaattt ataagttaca aatgtatctc ctttatctac
    16381 aatctcaaag tccatatctc tagcaaaccc atgttgagtc atgctatata aattttcttc
    16441 tattattgtt tcattatcaa aaagtttacc tactataggg aacaatatag gtgattgtct
    16501 accccaatat tttttattac catcccaaag aacgtttttg tcgtattttt tagaaaatat
    16561 tttagttagt tcagcaccaa aactgcttga ttcgataatt aaattttcat tttgtaatat
    16621 attcatttaa aatctccttt attatatttt atgagtatat tatactataa agtataaaga
    16681 ttattaaatt ttatttgata ttatatttga aaggtagatt ttagtatatt ttgaaaattt
    16741 tgttttgaaa atttgggatt atttaagaaa ttttgtatta taaatagaag gaaatagata
    16801 aattgtatag aataaaatat attaagataa gttaataatg ttaaatggga agtgagttta
    16861 tggaaaaacc aaatacaaaa gttgttaact ttcaagagta taaagacaga aaaaaccaaa
    16921 gagaagtaaa tagagacgaa cttttaaagc ttataaaaaa aattgtgact tcaaaatagg
    16981 gagttgttaa actccctatt ttaatgggtt taataataat taagacattc taagtccatc
    17041 tatataatct ttgaattgtg agaaatctga ttcactactt aattctatgg tttctgaacc
    17101 ttccgtataa aaattcttaa gatagatagt ataattatta tcaacacaat catgataaat
    17161 acatctatat ccatcttcat acaaactttt taatttagaa aaatcatcca caatatcatc
    17221 tccttaaagt tcttttaaat atatttttac aaaaagcatc taattgtatg caaattgaca
    17281 atgtaaaata aaggaaatga acataaaatg tagaataatt tttatatgta tgtaaaaata
    17341 aaaagaaaaa gttgaaattt ttagaaaata agagtatttt ttatctaaaa agagagttta
    17401 aaaaataaaa aaatgttggg aggataaaaa atgagtttat tagaaagtat atcaaaaaac
    17461 atatattccc tagataactc ttctattgaa aaaacaaagc agagattgga taggcttata
    17521 catccaactg gaagcttagg aaagatagaa gatatttgta tgcaattagc aggaatattt
    17581 ggcaatgaaa attttgacac aagtaaaaag gttataattg cttttgcggg agaccatggt
    17641 gtatatgaag aaggcgtagc acctgaccct caaaatataa caaaattaca gtttccaaat
    17701 ttttcgaaag gtctatgtgg agttggagta ataagtaaat ttgtaggggc agatgttgta
    17761 gctgtagatg taggaataaa ctgtgatgaa aaactggatg gggtattaga ctacaagata
    17821 agaaaaggga catcaaatat ggcaaaaggt ccagctatga gtaaacaaga agcaattaga
    17881 tgtttagaga taggtattga aatagcagag cagtgtatag aaaaagatta taaggttatt
    17941 ggtattgggg agatgggtat agcaaatact actccaagta cagcaataat atctgtaatt
    18001 tcagggtgtg acccgttaga agttactggt ataggtgcag gtcttaagaa agaaagacta
    18061 aagcataagg ctgaagtaat tagaaaggct attgaaatta ataatccaaa tccaactgat
    18121 ggagtagata ttttatctaa agtaggagga tttgaaatag gctcaatggc aggtgttata
    18181 ttaggttgta gtgctaatag gattccagta gtaattgatg ggtttatttc ttatgcagca
    18241 gcattaattg catataagat aaatccaaaa actagagaat atatgatagc atctcattta
    18301 tcagcagaat caggaactaa aagagcatta gacatattaa aattagaccc tctattaaat
    18361 atggatatga gattaggtga gggaagtgga gctgctttag catttgatat aatagaagct
    18421 tcaaactata cttataaaaa tatggcaact tttgatgaaa ttgacatggg aagataaata
    18481 taaaaattta atatgtagga gtgttttgat gagtaaaatt attctagtaa caggaggagc
    18541 taggtcaggg aagagtaact ttgcagaaaa actatgtttt gacagaaata ataatacagc
    18601 ttatatagca acctctattc catttgatga tgaaatgaaa gatagagtaa aaaaacatca
    18661 agagaataga cctagaaact ggaatactta tgaaatatat gaagatatat attccataat
    18721 taaggagata tataaagaac atgaaactgt tatactagat tgtgtaacat tattagtaaa
    18781 taatctaatg tttgaacaca atctaaatat agaagaatct agtcaagaag aaataaacaa
    18841 attagaacaa tacataaaag aacaagttgt caaacttatt gaagaaatag aaaaaacaaa
    18901 tctatacttt gtatttgtaa cgaatgagtt aggtatggga attgttcctg ggaataaact
    18961 tagtagaatt tatgctgata ttgtaggtcg tataaatcaa tatatagctt cgaaaagtaa
    19021 tgaagtttat tttgtagtta gtggcatagc tatgaagata aaggagtagt atgcaattat
    19081 gaaaagattt attttaatac tacagttttt gacaaggata cctataaaat taaatgtagg
    19141 atttgatgat gaattttata agtcaatagt atattttcca cttgtaggtt ttgtcatagg
    19201 tatattatcc tatttaatag gatggatttc tatgctttta tttgaaccat ttatagcttc
    19261 tataataata actctagctg gtgtcttaat tactggagga cttcatatag atggattagg
    19321 agatacattt gatgcaatat atagttatag ggataaagaa aaaatgcttg aaataatgaa
    19381 agattcaaga ttaggtacaa attcacttct agcaattatg tttgtacttt tattaaaggt
    19441 tggattcgtt tacgatataa taagtaataa ttcattgtgg gttataattt ttatgccaat
    19501 gattgcaagg ttaggagtaa tgttattgac atataaaact gtgacaccaa gagaaaaagg
    19561 aatgggaaat ttatttatag gaaaattaac tacaagtatg ttaataactg ctataatata
    19621 cacattattg atagttgctc ttattactaa atttatattt ttattaccta atatagtgtt
    19681 gattaaagta ttaggttcga ttatagtggt ttttgtattt ataatattat tcaaaaaaca
    19741 tatttataaa aaaattgacg gagtaacagg agatatatta ggctgtggaa tagaactttc
    19801 agagcttgta tatttaattt acatatattt gttaattttt atgttttttt aaagtaaaaa
    19861 gtggtgataa tatgataaga ttaatattaa taagacatgc actgacaaat gataataaaa
    19921 aaggaagatt atcaggccat ataaatagtt gtattagtga agagggcaaa ctacaaataa
    19981 ataaaattac cagatatctg agtaatgaaa acatagataa ggtttataca acaccatcta
    20041 cacgtactaa agatacagta gaaaaaatat caaaattgaa attattagaa atagaagaaa
    20101 aagaagcttt aagagaaatt agttttggag actttgaagg aagaactttt gaagaaatta
    20161 aagtcaaata tccaaatgag tttgaaaaaa tgataaaaga aggtaacaac tatagatatc
    20221 caaatggaga aagtctgata gactcttata aaagagtggc gaaagaaatt gataatataa
    20281 ttttagaaaa tgatagtaat ttagatacaa agacaatact tatatgttct catgcaggaa
    20341 caataagaaa tataataaca catttgattt ctggtagtta taagtatcac tggaacttta
    20401 aaattgataa tgcatctatt acagttttag aaattgatgg tggatttgct gtaatagata
    20461 aaatgaattt tacagacttc atataattta atcaaaacca aacattacaa tctgtgtttg
    20521 taatatatct gtaaaattag caatgaaaat agaatattag attaaaatta ggttcttaat
    20581 tagatgttaa aagggaaaaa ggttagatgc ctttgcagcc cccgctactg tgaaaccaac
    20641 gaaactataa ataccactgt cagtttgatg ggaaggttat agaagtagga tgaagttaag
    20701 ccaggatacc tgcctaattt aatttaaaac aagaggtctt cggggtgaaa gatttttaat
    20761 taatatatat aaaagtccta tctttagaag tattaggact tttttttatt tgttttttag
    20821 taaattttaa caaggaggga tggaaagtgg ggaaaaaaat aatgctacaa ggaacagctt
    20881 caaatgtagg taaaagtgtc ttagtagcag gtttgtgtag aatatttaag caagatggtt
    20941 atactgttgc tccttttaag tcacaaaata tggctttaaa ttcttttata actaaagaag
    21001 gattagaaat gggaagagca caagtatttc aagcagaggc tgcgaaaata gagccaatag
    21061 ctgatatgaa tccagtttta ttaaaacctt ctggaaataa taaaagtcaa gttattgtaa
    21121 gaggtaaagt tgtaggagaa atgccttctt ctaagtatca tgattataaa ttggagttag
    21181 tagatgtgtt gaaggaaaca tttaatagct taaattcaag gtatgatatt gtagttatgg
    21241 aaggggcagg aagtaatgca gagataaact taaaagatag agatatctct aatatgggta
    21301 tggcagaaat agctgatgca cctgtaataa tagtcggaga tattgataga ggaggcgtat
    21361 ttgcctcatt agcaggaaca atgcttttat taaatgaaga tgagaaaaga agagtaaaag
    21421 gtgtaataat caataaattc agaggaaaaa aagagctttt acaagatggt gtaaagatgc
    21481 tagaagacat cataaaagta cctgttatag gagtagtacc ttatacagat ataaaaatag
    21541 aagatgaaga cagtgtaact acgagattta agaaaaaaat gaataaaagt gatatacaca
    21601 tagaaataat tagaactcca catatgtcaa attttacaga ttttaatata tttgagacac
    21661 aagaagatgt aagtattaga tatgtagact atggagaatc tttaggaaat ccagatatat
    21721 taataatacc tggaactaaa agtactatag acgatttaac atatataaga aaaagtggtc
    21781 ttgaatcgca aatcaaaaat ttacatagtc aaggtaaatt gatatttggg atatgtggtg
    21841 gataccaaat gttaggtaaa aaattaaaag acccttatca tgttgaaagt gaaattgaag
    21901 aagttgatgg aataggtctt ttagatacag aaacaacgtt tgagaaagaa aagactacaa
    21961 cacaagtaga agctactatt gttgaatgta tcagtgaata catgaataac cttaagggaa
    22021 aaaaagtaaa aggttatgaa attcatatgg gaattacaaa gcgaggtaat gatgtaagaa
    22081 gtttagattt aataactaaa aaacttgata aagaagtgaa ttatactgag ggaagtgtaa
    22141 ataaagaggg gaatgtaatt gggacttatc ttcatggaat atttgatgaa ataagcttta
    22201 caagagcaat tttaaacaat ataagaaaga aaaaaggttt aggagaatta gaaagcaaag
    22261 taaactcttt taatgaattt aaggataaag agtatgataa gttggctgat tttttaagag
    22321 aacatttaga tatagagaaa atatatgaga ttatgaaata atgatgactt ttatttataa
    22381 tttaggtagg aggtgtatta tgaaaaaaat tttaattgcg ggtactaata gtggagtagg
    22441 taaaacaaca atatcattag gaataatgca agctttgatt aagagaaata tgaaagtaca
    22501 gccatataaa gtagggccag actatataga cccttcatat cacactttta taactggaag
    22561 acattctaga aatttagatt cttatatgtt agatgatgaa aaaataaagt atatagtaaa
    22621 taagtcctca aaagatgcag atatttcagt aatagaaggt gttatgggct tatatgatgg
    22681 ctttggaata gatttagata attgtacaag ttcacatact tcaaaggtat taaagtcacc
    22741 tgttatatta gttataaatg gtaaatctat ggcagcatct agtgctgcta tggtacttgg
    22801 atataaggaa ctagataaag atgtaaatat aaagggtgtt atagtaaata atgtaaagac
    22861 taatagccat tatcaaataa ttaagagttc tatagaaaaa tattgtaatg ttgaagtatt
    22921 ggggtatttt cctccaaata acaaattctc tttggaatct cgacatctag gattagttcc
    22981 tagtgtagaa atgaaatcac taagggaaaa attttatacc ttagcagatg aaattgaaca
    23041 ctatatagat attgacagaa taattgaaat atcagaaagc gatgaatttg agagtgattt
    23101 tgattttcaa actttaatag aaaataataa aataaaagaa catgttaata ataaatcaat
    23161 agctatagca tatgataaag cattcaattt ttattataga gaaaatatag agcttttaga
    23221 ggaacttggt ttagaaataa actattttag ccctcttgaa gatgtatcag tgccaaatgc
    23281 agattatatt tatattggag gaggttttcc agaaatattt ggaaaagaat tagaagcaaa
    23341 tgagtcaatg agaacttcca tattagaagc acataacaat aatataccta tttatgcaga
    23401 atgtggagga ctgatgtacc taggtgagaa actattgaat caagaagaac aagaatttaa
    23461 tatggttgga atatttagtg gaattagtaa gatgacatct tctttaaaga gatttgggta
    23521 ctgtattggt gtagccaagg aaaatactct tttatctaaa aaaggggaat ctattaaagg
    23581 gcatgagttt catcattcaa tatttgaaag tgatgaaaaa tgtgtatatt caatgagaaa
    23641 agaaagagat ggaaatatag tagaagagtg ggatggtggt tatagtaaag gaaacacttt
    23701 ggctacatat cttcatactc atttttataa taacttaaat tgtatagaaa attttttgaa
    23761 aaaaggaaat gaataaatga gtattttatc tatttatata ggatatgtga cagacttaat
    23821 tgtaggagac ccatattctt ttcctcatcc agtgagattt attggtaaac ttataaattt
    23881 tactcaaggt ataataagaa aggtctctaa aaatgatagg cagttaaaat ttgggggatt
    23941 tatattatgg tttataaccg ttggaataac atatttaaca acttatgtta tagtgaagtt
    24001 atttagtttt aatatattat tgagtattgt agtgaatagt tttataattt atacaacatt
    24061 agccacaaaa tgtttaaaag acgaagcatt aaaaatttat aatgtattaa aaactgaaga
    24121 tatagaaaaa tctaggattc agctatctta tatagtaggt agggatacta ctaatttaag
    24181 tgaacctgaa ataataagag caactgtaga aactgttgca gaaaatactg tagatggtat
    24241 aattgcgcct atgttttatg catttattgg aggagcacca cttgctatgg cctataaagc
    24301 tatcaataca ttggactcaa cagttggata taaaaataaa aagtataaag atattggttt
    24361 tgcatctgct aagattgatg atatagccaa ttatatacca gctagaatat ctgtcattct
    24421 tatgacaatt ggaagtttct ttttaaatta taactatagg aactgtttta aaatctctat
    24481 tagagataga aagaatcata aaagtccaaa ttgtgctttt tcagaaggtg cagtatctgg
    24541 tgctctagga atacaactag gaggtaccaa cgtttatttt ggggaaaaag tatataagcc
    24601 aacaataggg gataagctta gggagataga taaagaagat ataattaaaa ctaataaaat
    24661 tatgtatgca tcttcatttg tatctatgtt ggtatttaca ctaatatatg tgttatgtag
    24721 gatgatttta attaacttaa ttatataaat tattgtttgt taacttaagg gggacataga
    24781 tgaaggattt agggcatggt gccaatgtag atgaaatggc aagattatat gggaaaaacc
    24841 caaaagaaat aattgatttt agttcaaaca taaatccaaa tgtattacca aatttagaga
    24901 gatacatttt aaaaggatta gaagagtgta ggaattatcc agatataaat tatacaaact
    24961 tgagagaaaa tatatcaaaa tatatagata taaatccaga ctttataata cctggcaatg
    25021 gtgcaacaga agtgatttat ttgcttatga agagtattaa aaaaaaattg gctataataa
    25081 atccgacttt ttcagagtat agaagaagtg ctgagttaaa taatttagat attatagatt
    25141 tagagttaga tttagaaaat aactttaaac ttaatataga tataataaaa gaaaatataa
    25201 aaagatttga tagtttattt atatgtaatc ctaataatcc gagtggtaat gtccaagatt
    25261 taaaggaact tgttcattta ctagataaac ataataaggt actaataatt gatgaaactt
    25321 tcatggaatt tgttgaagat gaaagtaaat atagtttggt taaatatata gaatcaaata
    25381 aaaatatatt tataattaaa gctgtgacta agttttttgg tatgcctggt ttaaggctag
    25441 gatatggtct tacaagcaac actgaaatta tgaacaaaat ttatgaacac aaagagccat
    25501 ggactatcaa ttcttttgca gatatactat ccaattttat ttttgaagat aaagagtata
    25561 ttaaaaatag taaagaatat tacatagaag aaagaaaata tatgttacaa gagttgagaa
    25621 acataagaaa tataaaggtc tatgatacag atgctaattt tatcttgata agaatatata
    25681 aaaaaacaac taaagaattg aaaaaagatt tgtttaagca aggaaacata ttagttagag
    25741 atgcatctaa ttttatagga ttagatgata gttttataag agtggctata aaatctcatg
    25801 aagataacaa aattcttata gaaaatatga aaaatttatt gggtgattaa aatgaaatct
    25861 tatggaatat gcccagcatc ttgtggagaa tttgtacaag gtataattga tgatgaagaa
    25921 tatttatgtt catatgcaat agatatgtat tctaaagtgt atattgaaga aaaattagta
    25981 gatataaatt tagggagata taagtcaagg ttagcaattg aaaaggtatt tgaaaaattt
    26041 aatttaccta aaaaatatac taaaaatatt tctttaaata taaatagtaa aatacctgtt
    26101 ggaaaaggaa tggcaagttc aactgctgat ataggagcta ctataaaggc aacattatca
    26161 ttgatagata aagatttaag tagcgaggag atttctaaat tagcagcaga aatagaacca
    26221 acagattcta tatttataga taaaaatagt atatttaatc ctctaaatgg aactgttata
    26281 aaatacctag gaaatctgac caatgcgaaa gttgtaatac ttgaaccaaa caaagtacta
    26341 gatactatga aaattagatt gaggcaagat tataataaat taaaagtaga gaataaagaa
    26401 gtaataaaaa aatcatttgc tcttttagaa gaaggattga aaaaaaataa tttatctcta
    26461 gttggagagg cttgtacttt gagtagctta gcaaatgaga atatagaaaa aaaagagtat
    26521 ttaaatgaaa taataaaaat atcaaaaaaa tatggagcat atggagtaaa tattgctcac
    26581 agtggaactg tagtaggtat tttaattgat aagtttatga atgataaaaa gatgatagat
    26641 gccttatgtg aatctaacat aaattctgtc tataataaaa tttatacaca aaacataatt
    26701 aatggaggaa ttaaggggga aatagaatgg aatacataaa gaatcctatg aaaatagaag
    26761 aaaaaagttt tgaattgata caagaaataa tagacgaaat tagaccagat tataaattta
    26821 agaataatat cgaagaaaaa ataataaaaa gagctataca tactactgct gattttgatt
    26881 acttagatat attaaaaata tcagaagaag cagttgatag tataatagat gcattaaaaa
    26941 ataatgcaag tatttatact gatacaaata tggctcttag tggtataaat aaaagaaagt
    27001 tagaatcatt aggatgtaag tataaatgtt tagtggcaga tgatgaaact gtaaaattag
    27061 ctaaagaaaa aggaattact cgttctatgg cagcagtaga aattgcagct aaagaagaag
    27121 gaagaaaaat attcgtttta ggtaatgctc caacagcttt atataaagtt atggagatga
    27181 aatcagaagg tagattagat ttagatgcag ttataggagt tcctgtaggt tttgtaggag
    27241 cacaagaatc aaaagatgaa gttgaaaaaa cagatatacc atatataatt tctaaaggta
    27301 gaaaaggtgg cagtaattta gcagcagcaa tagtaaatgc tatattatat actatgtagg
    27361 tatctatatg gaagaatatg tatatataga tggaaaaaaa tatagaagag gatatactac
    27421 aggttcatgt gcaacaggtg catctaaggc tgctgtatat atgttgatta caaaaaatag
    27481 aatcaatact ataaatatag atactcctaa ggggatacca ttgctactaa aagttgataa
    27541 tataaatatt tctgatacat ttgtggaatg ttccataaaa aaagatggtg gagatgatat
    27601 agatgcaact cataccatgg atatttatgc aagagctgaa attgtagcta aaaatgataa
    27661 gaacaaaggg tatcttactc taaaggatat agatagttta agcaccaata gtgagtgtaa
    27721 aagtgagctc tataagttta ttagggttta tggcggaact ggtattggtg tggttacgaa
    27781 aaaaggtctt agcgtggatg ttggtaaacc tgctataaat ccgactccac ttaaaatgat
    27841 aaatcatgaa ataagaaagc ttataggaga taattttgaa tctatacttg gaaatgataa
    27901 agttttaaaa ataacaatct ttgctccaca aggagaaact gtagcaaaaa aaacatttaa
    27961 tccaagatta ggtatagttg gaggaatatc tataataggt acaactggga ttgttgaacc
    28021 catgagtgat gatggttgga agaagtctct atctatagaa cttcagatga aaaaagaaca
    28081 aggcttagat aaaataatat tggttccagg aaatcatgga gaacagttta taagagaaaa
    28141 attaaactta gatattaagt atgtagttag ggtaagtaat tttataggct atatgattaa
    28201 agaagctcaa agaataggat ataagaaaat cttaatggca ggtcatatag gtaaatttat
    28261 aaaagtatct gctggtattt ttaatactca tagtaaggta gcagatgcta gaagtgagat
    28321 attagtggca aacttagcct taatgggagc tagatacgaa tttttaaata aaataaatca
    28381 atgcgtaact actgaagaag ctgtagagct aataaataat agtgaatata gagaagtata
    28441 taatatatta agtaataaat gtaaggagag agtaaagcaa tacttgaatg aagattcaga
    28501 tgatattgat gtggaagtaa tcatattttc aatggataag tctttattag gtaaatcgga
    28561 taatacagat gatttagtgg aggtctttat atgataaaca taataggtct aggacctggt
    28621 aacttagact atataacaaa aaaaggtgaa aatttaattt ccacttctga tgtattaatt
    28681 ggtggtaaaa gaaatttaga gtctataaaa aattttgaag gagaaaaaat agttctggat
    28741 tctaatttaa gagaaattat agagtacata aataacaata aagaaaagca aatatcaata
    28801 atagcatctg gggacccatt aatatatggg ataggtagat atctatctaa aaatattgat
    28861 aataagatgt taaacgtggt ttctgggatt agttcattac agtacatatt ttcaaaaata
    28921 tatgtagata tgaatgatgt atatattaca agtagtcatg gcaaagtacc agattttgat
    28981 tacatattat cacataaaaa agtatgtatg gttacagatt caaagatagg accaaaacaa
    29041 ataagtagag aaataataga tagaaatcta aataaaatta ttgttgtagg agaaaatctg
    29101 tcatatgaca atgaaaaaat aacaatagca aaaccagaag agataataag aatggacaat
    29161 tttgatatga atgttgtggt gattttagat gaggaatagt gaatttataa caggtaaagt
    29221 tccaattact aaggaagagg ttagagcaat atcaataagt aagttaaatt tagtaaatgc
    29281 taaaaacctc atagacatag gggctggtac aggaagtgta agtgttgaag ctgcatataa
    29341 ttatcctaat ttaaaggtta tatctataga gaaaaatgat gatgctatag agcttataga
    29401 gaaaaataaa aaaaaattca acttaaaaaa tatagaagtg ataaaaggat atgctccaat
    29461 taatctaggg ttaaatgtta aagttgagtc tatttttctt gggggaacag gaaataatct
    29521 ggaagaaata atagaatggt ctaaaaaaaa tctcgttaca ggaggaagac ttgtagctaa
    29581 ttttataata atagatacat ttaatcaaac tttaaaactg cttaaaaagc atggatttaa
    29641 agaaatagat gtatgtgttt tgaatgtttc taaactagag aaactaggaa aaggtgaata
    29701 ttttaaacca ctaaatccta tatatataat atcttgtgaa aaaggggaaa atgatgatga
    29761 ataaagtaca ttttgtagga gcaggacctg gagataaaga gctgataaca ttaaaaggat
    29821 acaaattatt aagtaatgca gatgtagtga tatatgcagg ttcacttgtt aatcctgaac
    29881 ttttggaata ctgtaaagaa gattgccaaa tacataatag tgcacatatg gatttacaag
    29941 agataataga tgttatgaga gaaggtatag aaaataataa atctgttgta agactgcaaa
    30001 caggtgattt ttctatatat ggttcaatta gagaacaagt ggaagattta aataaattga
    30061 atatagatta tgactgtact cctggagtaa gttctttttt aggggcagca tcatctttag
    30121 gggttgaata tacagttcca gaaatttctc aaagtgtaat tataacaaga atggaaggta
    30181 gaactccggt tccagagaaa gaatcaatac aatcatatgc taagcatcag acatctatgg
    30241 taatattttt atctgttcaa gaaattgaga aagttgtgtc taaattactg gaaggtggat
    30301 atccaaaaga tactcctata gcagttatat acaaagcaac ttgggcagat gaaaaaatag
    30361 ttaaaggaac tttaagtgat atagctgtaa aagttaaaga aaacaacata aataagactg
    30421 ctttaataat ggttgggaga tttttaggag aagagtataa taattcaaag ttatatgata
    30481 aggatttcaa acatgaatat agaggttaat attgctataa tctgtattac agaaaatgga
    30541 aaaaaattag ctctaaagat aaattctcta gtaaataact gtgttatata tcaagtaaaa
    30601 aataaaaaaa gtaatttagt tgttgaagaa acatgtgtgc atacagttca aaagaagtta
    30661 agtgattttg taggagaaat atttagcaaa tttgattaca tagtattcat aatggctaca
    30721 ggtatagttg tacgaagtat agcaccatac ataataagta agtttagtga ccctgcaatt
    30781 ttggtaatgg atgaaaaagg caaaaacgta ataagtttat tgagtggtca tattggaggg
    30841 gcaaacagct tgacacagta cataagtagt cttattgatg ctaatccagt aataacaaca
    30901 gctactgata taaatcaaaa agcttcctta gatatgatag caaaaaaact aaatgcacat
    30961 attgataatt ttagagaaag tgtaaaagaa gtaaattcta tgctagtgaa taataaatgt
    31021 gttggattat atattgatga aggatatcaa gttgatacta ggggatttaa acttatagaa
    31081 agtaaggatg agttaagaga tagtttttca aacacatata tagaaaaatt agttgtagta
    31141 agcaataaaa taaattgtat tgaagatatt gagaatttaa acctagataa taaatttgaa
    31201 cattgctata gtaccaaaaa aataataaag gtaattccaa gagatatagt gataggaatt
    31261 ggttgtagaa gaaacacaga tagtgaactg atgtataatt cacttgaaga gtttcttaat
    31321 aaacaaaata tagatattag agcaataaac acagttggaa gtatagaact gaaaaaagac
    31381 gaaaaagcaa taattgaatt atcaaaaata ttgaaggtgc aatttaaaac attttcagta
    31441 gatgaaatat ctaaggttga taatcttttt gataaatctg attttgttaa aaaaaatgtg
    31501 ggcgtgtatt cagtagcaga acctgtagca tatcttttga gcaaaggtaa tttgttagtt
    31561 gaaaaacata aatataaagg aataactttt tcagtgggga gaataaaaat atgatatacg
    31621 ttgtaggaat tggaccagga agtaaagata caatgacttt agaagcaata aaagcaatag
    31681 aagattctga ggctattgtt ggatataaga cttatataaa actaatagaa gaatttatac
    31741 aaggtaaaga aataatccaa aatggtatga ggcaagaagt tgatagatgt aaacaagcag
    31801 tagaggaagc aaaaaaagga aaaaaagtag cagttgtaag cagtggtgat gctggaatat
    31861 atgggatggc aggtcttatt ttagaattaa tatctaaaga agatgaagat ataaaagtaa
    31921 aggtaattcc aggtgttact gcaagtattg gtgcagcagc tatacttgga gctcctatta
    31981 tgcatgattt ctgtcatata agtttgagtg atttaatgac tccgtgggaa gtaattgaaa
    32041 aaagattgag attggctgct gaagccgatt ttgtaatttg tttgtataat ccaagaagta
    32101 agggaagaag tgaacatcta agcaaagcat ttaaaataat gggtgagttt aaaggtggag
    32161 atacacctgt tggagtggta aaagatgtag gtagagaaaa agaagaaaag tttgtatgta
    32221 cttttgaaaa tatggacttt gaaaaagtcg atatgactac aatggttata ataggaaata
    32281 agtctaccta tattaatggt gaattgatga taactccaag aggttataca gtatgatttt
    32341 aattttagga ggaacttcag atagtttatc tatatgtgat aagataaatg aactgaaaaa
    32401 tcaaccatat atcctttcag ttactacaga ttatggaagg gaacttgcac aacaacatgc
    32461 agagaatgta attttaggta agcttggaaa agaagatatg ctaaaattta taaaagataa
    32521 taatatagta aaaataatag atgccactca tccttatgca gttgaagtat ccaaaactgc
    32581 tattacttgt gcaaaattat tgaatataga ttatataagg tttgagagaa aatctttaat
    32641 tgaagaaata gattatgata ataaatttat agtagataca attgaagaag cctgtgaaat
    32701 agcaaataaa atagggacaa atatattcat aggaacagga agtaaaaatt tgaacagatt
    32761 tatagaggaa ataagtgata aaaatttagt tgttagagtt cttccaacga gtgaagtcat
    32821 attaagttgt gaaaaattag gtttaagtgc agataatata gtagctatga aaggtccatt
    32881 tagccaatat ataaatgaag aaacatataa acattacaat atagatttag tcataacaaa
    32941 agaaagtgga atagctggtg ggttcttaga gaaggtaaat gcatgtaaaa ctttcaatat
    33001 accagtaata attataaaaa gagaatcgat taactacgaa aatgtattta atgacataaa
    33061 taaaattaaa gacatacttg tgtaattttt aggaggagaa tatatgaaga aagccttatt
    33121 agtagtgagt tttggtacaa gctatcatga aacgagagaa aagacaatag atatatgtga
    33181 agataaaata aaaaattcgt taaaaagcca tgattttttt agagcttata catcaaatat
    33241 gataataaat aaaataaaaa aaagggatgg aatagaaata gataatccaa tacaggcttt
    33301 agataaaata tatgaacaag gatatgatga agtaataatc caaacacttc atattatatg
    33361 tggcgaagaa tttaacaaat taaaagaaca agtagatgga tattcttcta aattcaaaaa
    33421 gattgtatta ggaagacctt tgcttacaca tattgaagat tatcaggaag ctgtagaggc
    33481 aattaagcat caaattcctc atatggaaag taatgaagca gtagtattta tgggacatgg
    33541 aacacttcat gaatcacatt cagcgtatcc agcattggaa tacatgctta gggataatgg
    33601 aataaatgct tatgttggta cagttgaagg gtatccagaa atagaacatg taataagaag
    33661 attaaaagaa ggaaatataa aaactgtaaa tttgatgcca tttatgttag ttgcaggaga
    33721 ccatgctata aatgatatgg caggagatga agaagattct tggaaaacaa tattagaaga
    33781 aaatggattt aacgtaagag ttcatttaaa aggactagga gaaaattctt atatacaaga
    33841 taaatttgtt cgtcatgctg taaaatgtgc agagaatgcg aagttctatg gtataggagc
    33901 agggccagga gatggttctt tacttacgat aaaagcggtt aatactttaa agaaactgga
    33961 tattctatac actccagaat caaaaaaggg tggagatagt ctagctttat caatagttag
    34021 tgaatattta cctgaatcag tggaaataaa gtctaggcat ttcccaatga gttttgatgg
    34081 gaatgaaaaa agtttagctt gggacaatgt ggcacaagaa attatatcag atgttaatga
    34141 tggtaaaaat gtaggatttg ttacattagg agaccctatg atttatagta catatgtata
    34201 tataatgaaa agaatattag gagaggtaga agtcgaaact ataccaggaa tttcatcatt
    34261 ttccaatata gcatctaatc agaattttcc attagtcatg gataaagaag ctttagtggt
    34321 aattccttgt actatggaag atgataagat agagtatgct ttgcaaaatt ataattcaat
    34381 agtattgatg aaagtatata aaaattttaa agaaataata aaaaaattag aaaagtatga
    34441 tttaatagaa catgctattt tagtaagtaa ttcttcccaa gaaagcgaaa atgtgtttac
    34501 agatttaaaa gaagcgcacc ttgaagataa gatatcttat ttttctacta tactagtaaa
    34561 taaagataat aaaataaaat aggtgaaagc aatatttaaa ggagacttaa tatgaatata
    34621 gtggttggaa ctaggggtag taatctagcg cttatacaaa cagaatgggt aataaatgaa
    34681 ttaaaaaaga agtatccaga aattagtttt gagattaaaa tcataaagac aaaaggagat
    34741 ttaattcaaa atgtttcact ggataaaata ggtgataaag gattgtttgt caaagaaata
    34801 gagcaacaat tattagatgg aaaaatagac atagcagtac atagtatgaa agatatgcct
    34861 tcttatttag cagatggact aaaatttgct catacgccta agagagaaga cccaagagat
    34921 gttctaatac ttagagaagg atataaaaat ttagatgatt tacctcatgg agcagtaata
    34981 ggaacaggaa gtaaaagaag gaagtttcaa ttattgaaac aaagacctga tttaaatata
    35041 gtacaagtta gaggtaatgt ggaaactagg atacgaaaaa taaaagatga aaatatgcat
    35101 ggtattgtgt tggcagcatc aggaataatt agagctaatt tgcaagataa aataagttct
    35161 tatttacctg ttgatgttgt tattccagca cctgcccaag gagctttagc tatagaaatt
    35221 agaagcaatg atagtacgat agagggaata gtaaattcac tgaaggatga gaatacagaa
    35281 atccaaatat tggctgaaag aggtttttta gatggtgtaa atggaagttg tcatattcca
    35341 atggcagctt attgtgaaat aatccaagat aaaatacatc ttactggctt atatggagat
    35401 tcagaaggaa aaaaagtagt aatcaagtct atagatggag acatttcttc tcctagagag
    35461 ttaggtttaa agttagctaa acttgtattg aaggagtatg agaattatga agggtaaggt
    35521 ttacttagta ggggcaggac caggtgatta taaactcatg actttaaaag gtttagagtg
    35581 tataagaaaa tcggatgtaa tagtatatga tagattagca aatagtagtt atttaagaga
    35641 agctaagcct gattgtgaac ttatatatgt aggcaaggca tcaagtaatc atattttgcc
    35701 acaagaagat ataaatagaa ttatagcaga aaaagctaaa gaaggaaaaa ttgtaactcg
    35761 tcttaaagga ggagaccctt atgtatttgg tagaggggga gaagaagcag agactttagt
    35821 agatgaaggt atagaatttg aagtagttcc aggtataact tctgctatag gaggtttatg
    35881 ttatgcgggt attcctataa cacatagaga ccatgcttcg tcatttcatg taataacagg
    35941 tcatttgaag ggtgatgata gtggagaatt aaactggaat gctcttgcaa ataacaaagg
    36001 tactcttgtg tttttgatgg gaatttcaaa cctagagaaa ataagtgaaa acttaatgaa
    36061 agaaggaaaa gataaggata cacctgttgc tcttataagt tgggcaacta gatataatca
    36121 aagagttgtt acatctacac ttgaaaatgt ttatgaaact gctattaaag aagaagtaaa
    36181 accgccaact cttatagtag ttgggagtgt tgtaggactt agagaaaagt tgaacttctt
    36241 tgaaagtaag cctttatttg gaaaaagtat tgctgtaact aggtctagaa atcaaaatag
    36301 tgtattggta gaaaaaataa tggatttagg tggaaatcct atagaaatac ctactattaa
    36361 ggtagaaaaa atacaaaata atattaactt agaaaatgaa ataaaaaata taaataagta
    36421 taattatcta atactaacta gtaaaaatgc tgtagaaatc ttctttgaaa agatgtatga
    36481 aatgaatttt gatttaagga tattatctaa tttaaaggta tgtgctatag gttctgctac
    36541 atcaaatgag ttgaaaaaaa gaggaattat agcggatata gtaccaaaaa aatttgtagc
    36601 agagtcactg tatgaagagt tagcacctat tttaaatgat gacgataata tacttatacc
    36661 tagagcagaa aatgctagag attatctggt tgacaaactt aaggctattt gtgaagtaac
    36721 agaagtacat atatatagaa cagtaataga tgaggagaaa aaaggtgaga tattagatat
    36781 tttaaattct aatgacatag attatataac atttactagt tcctcaacag taaataatct
    36841 tgttgaaatt ataggaaaag aaaatattaa aatattagaa aaaataaaaa cactatctat
    36901 aggtcctata actacagaga caataaatag tttgggaata aaattgtaca aagaatctgc
    36961 tacatctact atagattcgt tagtagattt aattataaaa aattaatttt ggaggcttga
    37021 atatgttaag aagaccaaga agactaagag caagtaaagc aatcagaaat ttggtaagag
    37081 aaactaaggt gaatgtagaa gacttaatat atccactgtt tgtagtagag ggggaaaata
    37141 taaaagaaga aattaaatct ttaccagatg tctatcattt atcaatagat atgctagaag
    37201 acgaaataaa agaaataagt gagttgggaa ttgaacatat aatgttattt ggtgttccag
    37261 atgaaaaaga tgagtgtgct tcacaagctt ttaatgataa tggaatagtt caaaaggcaa
    37321 ttagaaaggt aaaagaaata aattcagata taaatgtaat aacagatgtc tgtatgtgcc
    37381 aatatacaag tcatggacat tgtgggattt taactgatac tggttatgtt gataatgata
    37441 aatcattgga gtatttggct aaaatatctg taagccatgc taaagcagga gctgatatgg
    37501 ttgctccatc tgatatgatg gatggaagaa tacaagcaat aagagaagct ttagatgaaa
    37561 atggattaga aaatgttgga atcatggctt atagtgttaa gtatgcttca aattattatg
    37621 gaccttttag agaggcagct cattcagcac catcttttgg agatagaaaa acatatcaaa
    37681 tggacccagc aaatagcaat gaagctataa tagagactga acttgatata ttggaaggtg
    37741 cagatatact tatggttaaa cctgcattat catatttaga tattataaga agagttaaag
    37801 ataattttga cttacctcta gcagcttata atgtaagtgg agaatactct atgcttaagt
    37861 ttgctgtgaa ggaaggttta ttaaatgaag atgtaattct tgaatcaatt acatctataa
    37921 aaagagcagg agcagatata ataataactt attttgctaa agacattgct aaactgttga
    37981 aaaaataaat tctagtagta taaagaatat caaaaagtat aggaattgta attaactatt
    38041 atacagtagt tttgtttaga catttattga agaataatag aaactaattt gcaatttcta
    38101 tactttattt atgtaactaa ataaaatttg gaggtagaag atgctatatc ctattaattt
    38161 aaaattagat gagttagatg tagttataat tggtggtgga gaagttgcat ataggaaatg
    38221 taaaaatttt ttagagttta acaaaaatgt tactatagtt tctaaacaaa ttttaaataa
    38281 attttatgat ttaaaaggaa atataaaaat aataaaagat gattataaag aaatatatat
    38341 aaaagatgct tctgtaataa ttgctgccac aaataataga gagttaaata tggaaatagg
    38401 attatattgt agagcaaaaa ataagctagt gaatgtagtt gataatatag aaatatcgaa
    38461 ctttactgtt ccatcatata taaaaagagg agatttactt ataagtgtgt ctactggggg
    38521 caagagtcct tcattatcat caaagataaa aaaagaaatt gaaaagagat atactgaaga
    38581 ttatgaggaa tatttaaatg tattaggaga tataagaaaa aaggttataa aaaaatacga
    38641 agataaaagt aaaagaaaag atgtgcttaa tatgttggta acacttgacc tagaagaact
    38701 taagaagttt gatatataaa aaataaaata aaaaatgtta aaaaggtgtt gacggattat
    38761 ttttaagatg atatagtatt acttgtcctt aagaaaaggg caaacataaa aaatgaactt
    38821 tgaaaattaa acagtaggtt aatttat
//
LOCUS       DAEMHZ010000022        37986 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000022 DAEMHZ010000000
VERSION     DAEMHZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.022"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            372..509
                     /gene="rpmH"
                     /locus_tag="KPK72_001042"
     CDS             372..509
                     /gene="rpmH"
                     /locus_tag="KPK72_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG2488282.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            587..931
                     /gene="rnpA"
                     /locus_tag="KPK72_001043"
     CDS             587..931
                     /gene="rnpA"
                     /locus_tag="KPK72_001043"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG2488283.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            963..1214
                     /gene="yidD"
                     /locus_tag="KPK72_001044"
     CDS             963..1214
                     /gene="yidD"
                     /locus_tag="KPK72_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG2488284.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            1246..1953
                     /locus_tag="KPK72_001045"
     CDS             1246..1953
                     /locus_tag="KPK72_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2488285.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1922..2551
                     /locus_tag="KPK72_001046"
     CDS             1922..2551
                     /locus_tag="KPK72_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG2488286.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2663..4042
                     /gene="mnmE"
                     /locus_tag="KPK72_001047"
     CDS             2663..4042
                     /gene="mnmE"
                     /locus_tag="KPK72_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG2488287.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4140..6032
                     /gene="mnmG"
                     /locus_tag="KPK72_001048"
     CDS             4140..6032
                     /gene="mnmG"
                     /locus_tag="KPK72_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG2488288.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            6025..6744
                     /gene="rsmG"
                     /locus_tag="KPK72_001049"
     CDS             6025..6744
                     /gene="rsmG"
                     /locus_tag="KPK72_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG2488289.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6907..7692
                     /gene="noc"
                     /locus_tag="KPK72_001050"
     CDS             6907..7692
                     /gene="noc"
                     /locus_tag="KPK72_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG2488290.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7827..8600
                     /locus_tag="KPK72_001051"
     CDS             7827..8600
                     /locus_tag="KPK72_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG2488291.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8602..9465
                     /locus_tag="KPK72_001052"
     CDS             8602..9465
                     /locus_tag="KPK72_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG2488292.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9498..10640
                     /locus_tag="KPK72_001053"
     CDS             9498..10640
                     /locus_tag="KPK72_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2488293.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10774..11349)
                     /locus_tag="KPK72_001054"
     CDS             complement(10774..11349)
                     /locus_tag="KPK72_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG2488294.1"
                     /translation="MKNKKIMLVLSILSISIFSVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11609..12901
                     /locus_tag="KPK72_001055"
     CDS             11609..12901
                     /locus_tag="KPK72_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2488295.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            13006..13608
                     /gene="yedF"
                     /locus_tag="KPK72_001056"
     CDS             13006..13608
                     /gene="yedF"
                     /locus_tag="KPK72_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG2488296.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13681..13944
                     /locus_tag="KPK72_001057"
     CDS             13681..13944
                     /locus_tag="KPK72_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG2488297.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            13959..14144
                     /locus_tag="KPK72_001058"
     CDS             13959..14144
                     /locus_tag="KPK72_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG2488298.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14247..15443
                     /locus_tag="KPK72_001059"
     CDS             14247..15443
                     /locus_tag="KPK72_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2488299.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15657..15935
                     /locus_tag="KPK72_001060"
     CDS             15657..15935
                     /locus_tag="KPK72_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG2488300.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            15972..16406
                     /gene="ssb"
                     /locus_tag="KPK72_001061"
     CDS             15972..16406
                     /gene="ssb"
                     /locus_tag="KPK72_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2488301.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16425..16652
                     /locus_tag="KPK72_001062"
     CDS             16425..16652
                     /locus_tag="KPK72_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG2488302.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16795..17115
                     /locus_tag="KPK72_001063"
     CDS             16795..17115
                     /locus_tag="KPK72_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2488303.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17123..18079
                     /locus_tag="KPK72_001064"
     CDS             17123..18079
                     /locus_tag="KPK72_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG2488304.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            18090..20084
                     /locus_tag="KPK72_001065"
     CDS             18090..20084
                     /locus_tag="KPK72_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG2488305.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            20085..20534
                     /locus_tag="KPK72_001066"
     CDS             20085..20534
                     /locus_tag="KPK72_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG2488306.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20553..21881
                     /gene="dnaB"
                     /locus_tag="KPK72_001067"
     CDS             20553..21881
                     /gene="dnaB"
                     /locus_tag="KPK72_001067"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG2488307.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YAGIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            22086..22394
                     /locus_tag="KPK72_001068"
     CDS             22086..22394
                     /locus_tag="KPK72_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488308.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22476..23765
                     /locus_tag="KPK72_001069"
     CDS             22476..23765
                     /locus_tag="KPK72_001069"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG2488309.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            24091..24166
                     /locus_tag="KPK72_001070"
     tRNA            24091..24166
                     /locus_tag="KPK72_001070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24124..24126,aa:Lys,seq:ttt)
     gene            24173..24247
                     /locus_tag="KPK72_001071"
     tRNA            24173..24247
                     /locus_tag="KPK72_001071"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24206..24208,aa:Glu,seq:ttc)
     gene            24252..24327
                     /locus_tag="KPK72_001072"
     tRNA            24252..24327
                     /locus_tag="KPK72_001072"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24285..24287,aa:Val,seq:tac)
     gene            24361..24435
                     /locus_tag="KPK72_001073"
     tRNA            24361..24435
                     /locus_tag="KPK72_001073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24393..24395,aa:Thr,seq:tgt)
     gene            complement(24818..25810)
                     /locus_tag="KPK72_001074"
     CDS             complement(24818..25810)
                     /locus_tag="KPK72_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2488310.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25819..26877)
                     /locus_tag="KPK72_001075"
     CDS             complement(25819..26877)
                     /locus_tag="KPK72_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2488311.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDKIIKKSQKEKFK"
     gene            27002..28465
                     /locus_tag="KPK72_001076"
     CDS             27002..28465
                     /locus_tag="KPK72_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG2488312.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            28567..29364
                     /locus_tag="KPK72_001077"
     CDS             28567..29364
                     /locus_tag="KPK72_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2488313.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDISNVYEMK"
     gene            29377..31479
                     /locus_tag="KPK72_001078"
     CDS             29377..31479
                     /locus_tag="KPK72_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG2488314.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIRGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            31547..32026
                     /gene="rlmH"
                     /locus_tag="KPK72_001079"
     CDS             31547..32026
                     /gene="rlmH"
                     /locus_tag="KPK72_001079"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG2488315.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32093..33784
                     /locus_tag="KPK72_001080"
     CDS             32093..33784
                     /locus_tag="KPK72_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488316.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEE
                     EKRKKELFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEI
                     AQEQYRLWQQRAAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDL
                     VDMQDRGENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGI
                     TYSEAVVYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESIN
                     KSQNINNQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYED
                     ITEDEIIIDNIFCLVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNL
                     IRAYANTLYKQTKDGFANEKVFFTYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            34016..35488
                     /locus_tag="KPK72_001081"
     CDS             34016..35488
                     /locus_tag="KPK72_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488317.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVNQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEVFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDSVYGIPQHLLLEVIKNGKEFFTK"
     gene            35718..36527
                     /locus_tag="KPK72_001082"
     CDS             35718..36527
                     /locus_tag="KPK72_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488318.1"
                     /translation="MGDIHIVDDSLCKYSKTVLVLSNQEFAKVLKDQIISCIDDNIKI
                     LSNYSFAMLILPMNLLYGNDMKIIHEYSNNIFLNFFSRKDLNLDTYFEEFNSIEDIAL
                     ALNPGIESYIVFDEGEDLTKNLEERFKSFIDKGKVPLPAHKSDAFKFWLIIYGFITQA
                     LDIILSCKGFNLTPYLRYRVTLQYVLLLGDNVFDIEDLSNMLLKCKVAHVLYAIFDKS
                     EIKNISFVQFYKKLSEYKFSYKVEDDIKENININQIAEILNKNITLFLNYI"
     gene            36667..37824
                     /locus_tag="KPK72_001083"
     CDS             36667..37824
                     /locus_tag="KPK72_001083"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2488319.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFIDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCEIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIAKLNNYIDRLKKYTKDLHIMGERNSYSKTDNDATFMRM
                     KKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKFKYPKI
                     VADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKNDYYIC
                     KNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLENRTKNL
                     QVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
BASE COUNT        15329 a         3977 c         6649 g        12031 t
ORIGIN      
        1 gataacattt atgcacatta tcaacaagta tatttataat atcaaaatat atgtcaaact
       61 tcaatgattt gtaactaatt tatccacaat attaactaat tgttgataaa aatataaaca
      121 tgttaaaatt actttatttg tgtatataat tgaaaaaaat aatttagaat aaaattaaat
      181 agatttttac tagtattttc aataaaaaaa atacataata tatatatggt agattttgtg
      241 atataagcgt cccaaaaact cgttgacaac agtagtaaaa gtatttataa ttatatgtgt
      301 tatgtttact ttgagggtaa agttattatt tttataaacg gaaaataaaa tctgaaaggc
      361 ggtgcaaaat aatgagtaaa agaacttatc aaccaaaaaa gagacaaaga agtaaagaac
      421 atggtttcag aaaaagaatg aagacgtcta acggaagaaa cgtgttaaag agaagaagag
      481 ctaaaggaag aaatagatta actcattaat tacaaaaggg ccgctgcaaa ggtggccttt
      541 ttgtgtaaat aattacaatt catagctgtg tttatggagg ttaactatgg actttaatag
      601 gactaaaggg ttgaaaaaag actctgattt tagaaaagta tataaacacg gcaaatcttt
      661 tgcaaacaaa tatttagtaa tatatatact taaaaataaa tcagattata gtagagtagg
      721 tatttctgtt tcaaaaaaag taggaaaagc tattactagg aatagagtaa gaagattaat
      781 aaaagaagcc tacagattaa atattgatga aaaaattaag cctggatatg atatagtatt
      841 tatagcaagg gtatctagta aggatgctac ttttaaagac atagacaagt ctataaaaaa
      901 tttagttaaa agaacggata tttctattta attgtatgct ataaatatgg aggttaatga
      961 ctttgaataa aataatttat atattaaagg aaataagtaa gtatttatcg aatttatgta
     1021 tttatttagt gagattttat caaaagtata tatctccctt aaagggacct acctgtagat
     1081 tttatccaac ttgctcacaa tattcaatag aggcgtttaa gaaatatgga tttgtaaaag
     1141 gtatgtattt aacgataaga agaattttaa aatgtcatcc ttttcatcca ggaggttacg
     1201 accctctaaa ataaaagaga gtaactatta ggaggtataa aaaaattgaa tataatagga
     1261 aatttcttag gattgattct taaggtaata tttgaatttg ttaatcatta tggtatttct
     1321 ataatacttt ttacgatact tgtaaagatt attttattac cattaacaat taagcagact
     1381 aagtcaacaa aggcaatgca agatatacaa cctcgtataa aagagataca agaaaaatat
     1441 aaaaataagc cagaaaaaca aaatgaagaa attgttaagt tatatggaga agctaaaatt
     1501 aatccactat ctggttgttt acctttatta atacagtttc ctatattaat aggattattt
     1561 agcgttttga gagaaccagt tgctcatggc gtttttgcaa acaaagctgc atttttagca
     1621 gctgacaatg ggtttttatg gataaaaagc ttaacatcac cagattatgt attagcagta
     1681 ttttcaggag caagtgcata tgtcatgcaa aaagtaatga caccaaaaga tcagttacaa
     1741 ggctctatga aagtaatgac atatgtaatg gcaggtatgt ctttctattg gggatttata
     1801 ttcccagcag gacttacatt gtactggaca gtaagtaact tattctcaat agctcaatat
     1861 tacttaataa tgaatccttt aaaagctaaa ctggctgcta actctaagga ggaaaaagtt
     1921 aatgagaaaa agcctaaaat taataaaaag taagagtaaa gaggaagcta taagtaaagc
     1981 tatagcagag ttgaatttaa aagcagagga tatagaagta gaaatacttg aaaatccaag
     2041 taaagggttt ttaggtttta taggtgctaa agatggtact tatgaaattt ttgttatcga
     2101 gagggaagta gatgtagcta aaaattttat agaggtaatg ttgaaaaatg caaatgttga
     2161 tgccaaagtc aatgtatctc aaaaagataa tttaatcatg gttgatattg aaggcaaaga
     2221 agctgcaagt ttaattggta gaagagggga aactttagat tctattcaat ttttgactgg
     2281 attagctctt aataaaataa ataaagattc tcatacaaga gtacttgtag atatagaaaa
     2341 ttatagatca aaacgagaag aatctttaat aagatatgct aataaagttg ctagagaagt
     2401 agcgaaaaca agaaaaacta aaaaattaga ttatatgaat ccatatgaga gaagaataat
     2461 acattcagct cttcaaaatg acaaatatgt aattacatat agtgaaggaa ccgatccata
     2521 tagaagatta gtaattgagt gtaaaagata aagtcaacct cctaaacatt agggggtttt
     2581 attttttaat attattaaat cattaatgat tggttaaatg taaatctata tgtaaaaaat
     2641 tatgaattaa gaggtgataa atttgtttat agatgataca attgcagcaa tagctacagc
     2701 acctggagaa ggtggtatag gtatacttag aataagtgga gaaaaagctt tgaaggttgc
     2761 agaagaaatc tttaaatcta tgtctggaaa gagtattgaa gaatacaata agagaacatt
     2821 aatatatgga aatatagtgg ataatgaaaa tataattgat gaagttttat tggcatatat
     2881 gaaaggacca aattcctata caggagaaga tgttatagaa attaattgcc atggtggatt
     2941 tatttcggta aagaagatat tagaattaat actatcaaaa gatgttagat tagcagaggc
     3001 tggtgagttt actaaaagag cattcttaaa tggaagaata gatttatctc aagcagaggc
     3061 tgtaattgat gttataaaag ccaaaactga tatagctcat gaagttgctc aaaatcaatt
     3121 agaaggttct ttatcaaaga agataagaga attgagagat aaagtcactg agatattagc
     3181 tcatgtagaa gttgctatag attatcctga ggaagatatt gagcatataa catatcaaac
     3241 tcttaaagaa aaaactgatg aacttaaaaa agacattaaa aaattatatg atacagctga
     3301 aagtggaaaa atacttagag aaggcttaaa gacggtaatt gttgggaaac caaatgtagg
     3361 taaatcttca ctacttaatt ctattttagg agagaataga gcaatagtta ctgatatacc
     3421 aggaactact agagatgtta tagaagagtt tgtaaacata aaaggtatac ctttaaaaat
     3481 tgttgacaca gcaggtataa gagatactga tgatattgtt gaaaaaatag gtgttgaaaa
     3541 gtctaaagaa tcatttacta gtgcagattt gatagtaatg gttttagatg catctagaaa
     3601 gttgtcagaa gaagatatag agatattaga aaaacttaag gataaacaaa caatagtgct
     3661 gttaaataag aatgatttga aacaagagat tgaagaagaa aaaatactta aatatgtaga
     3721 aaataatagt ataataaaaa tatcagcttt acaacaagaa ggaatagaag aattgcaaga
     3781 taaaatagag tctatggtat ataaaggtag tatcaaaaat aattctagtt tagtagtaac
     3841 aaattcaaga cataaagatg cacttagtaa agcatataaa tctgcaacag atgctttaat
     3901 tgctttggaa caaagtatgc catttgactt tgtagaagtc gatttaaaaa atatatggga
     3961 ctatcttgga tatattaatg gtgatacagt aacagaggat ttactagata atatattcca
     4021 taatttctgt attggtaagt aatgtacaaa agtaaacttg tttttattat agagattttt
     4081 aaaaaatttg aagtgtttct catgaaacat taattttgaa aagaaaggaa ttataaatga
     4141 taaagtttga agcaggaaaa tatgatgtta tagtagtcgg agcaggacat gctggatgtg
     4201 aagctgcttt ggctactgca agaatgggat ataaaacttt gataataaca atgtctttgg
     4261 attccatagc attaatgcct tgtaatccat caattggagg gactggtaaa ggtcaattag
     4321 ttaaagaaat agatgcctta ggtggtcaga tgggattaaa tatagacaag acatatatac
     4381 aaagtagaat gttaaatact gccaaaggtc cagcggtaca ctctttaaga gcacaagcag
     4441 ataaatttaa atatcatgaa gaaatgaaaa agacattaga agatgaacca aatcttgata
     4501 tagctatgga tgaagttgta gaaatacttc atgaaggaaa tgttgtaata ggagttggaa
     4561 ctaaactagg atgttcattt aaatctaaag ctgtaatatt ggccactgga gtttatctga
     4621 actcaaagat atatatgggt gaagttgcat tttatgaagg tcctaatgca ttggggtatg
     4681 caaaatatct tacagatagc ttagttgagc ttggccttag aatgagaaga tttaagacgg
     4741 gaactccagc aagagttcat agagatagta tagacttctc tgtaatgtct ttacaagagg
     4801 gtgatgaaaa agtaactcca ttctcattta tgaatgaaaa tatagaaaaa aaacaagaac
     4861 cttgttattt aacaagaact acagaagaaa ctcaaaaagt tatactggat aatttaaaaa
     4921 ggtctgctat gtatagtgga gtaatagaaa gtactggtcc aagatactgt ccttctatag
     4981 aagataaagt agtaagattt agtgataaaa catcacatca attatttata gaaccagaag
     5041 ggttaaatac taaagaaatg tatatacaag gaatttcaac tagtttacct tttgaagttc
     5101 aattagatat gtacaaaact ataaagggac ttgaaaattg taagataatg agacctgcat
     5161 atgctattga atatgattgt gttgacccaa ctcaactaaa aataagtttg gaaataaagg
     5221 gtgttgagaa cttatttagt gcagggcaat ttaatggaac ttctggatac gaggaagcag
     5281 cagctcaagg gcttatggct ggtataaatg cagttagaaa gatagaagga aaagagccat
     5341 ttgtattaga ccgttcagaa gcttatatag gtgtcttatt agatgacctt gttactaaag
     5401 gaactaacga gccttacaga atgatgacat caagagcaga atatcgacta tatttaagac
     5461 aagataatgc agatatgaga ttaactcaaa aaggttatga tataggatta gttaaaaaag
     5521 atagatatga aaggttccta aataaaaagg ctgctgtaga gaaagaattt gaaagactaa
     5581 aaaatgaaag agttacacca aaagaagtaa atagtttgtt agaagaaaaa ggagcaacac
     5641 caataaaggt tggaatatct ctatatgaat ttttaaagag accagaggtt acttacgaat
     5701 tgcttgaaga attagggaaa ggcgctggag aagatgtttc aagagaagtt aaagaacagt
     5761 gtgtaataat aactaaatat gaaggttata tagagaaaca attgaaacaa atagaccaat
     5821 ttaaaaaact tgagaataaa aagctggatg agaaaataaa ttattcatct atagaaggtc
     5881 ttagattaga ggctagacag aaactagatg atataaaacc tatttcaata gggcaagcat
     5941 ctcgtatatc tggtgtttct ccagctgata tatcagtttt acttatatac ctagaacaaa
     6001 tcagaagaac tagaggtggc aaaggtgaat aatatagaac ttctgaaaaa cgggattgaa
     6061 ggttttaata tagatacgaa tgacagtatg ttagaaaaat ttaaaatcta tagagagata
     6121 ttagttgact ggaataaaaa gatgaatctt actggaatag aagatgaaaa agaagtgtat
     6181 ataaaacatt ttttagactc tatttcagct gtaaagaatg gatatataaa aaatgggatg
     6241 tctataatag atgttggtac aggtgctggt tttcctggaa taccattgaa aatatgttta
     6301 gatagcttag aacttacttt gttagattcg ttaaataaaa gaataaactt tttagaggaa
     6361 gttagtagag tactagagtt agataatatt acatttatac atggaagagc tgaagatttt
     6421 ggtaaagatg aagactatag ggaaaaatat gatgttgcta cggcaagagc tgttgctgga
     6481 ttaccgattc ttatggaatt ttgtgtacca tttgtaaaag ttggagggta ttttatttgc
     6541 ttaaaaggtc cgaatgctaa tttagagcta gaagaatcac aaaaggctat tgatgtttta
     6601 ggattggagt atattgaaaa aatagatgta gagttaccag aggtagattt aaatcataat
     6661 atattagttt ttaaaaaagt gatggaaaca ccagttaagt accctagaaa agctggtaaa
     6721 ccagccaaaa atcctattaa gtaatgaaaa atttattatt ggtaagctta attatttctc
     6781 aggaaataat taagcttact gtaaaaaaac aaaaataaag gaattattaa agtagatgca
     6841 gaaataaatt tgtatgtaca tatagagaac aaaaataatt tatatctaaa ataagagagg
     6901 tatgttatgg aagataaaag agttatggag atacctatag aagatgtagt tccaaatcca
     6961 tatcaaccaa gaaaaatatt ttcacaagtt tcattggagg aattgagcaa ttctataaag
     7021 gtatatggta taattcaacc tataacagtt agagcaaaag atggcaaata tgaactaatt
     7081 gcaggagaaa gaagattaag agcagctaaa ttagcagagc ttaaaaccat accagcaatt
     7141 ataaataata tgaacgatga atcttctgct gttctagcgt tattagaaaa tttgcaacga
     7201 gaagacttaa atttcattga agaagcaatt ggttatgaaa acttaataaa agaacatgct
     7261 tttactcaac agcaattagc agaaaaattg ggcaaaaatc aatctactgt agcaaataag
     7321 cttagaatat taaagcttcc aaacgatata aaaatgaagc taatagagaa taatttaaca
     7381 gaaagacatg ctagagcatt tcttaaacta ccaagtgaag atttaatgca aagtgtgtta
     7441 gacaaagtca taaaaaatga attgactgtt aaaaaaacag aaaaattgat acaagatgta
     7501 ttagaagaaa ctaaagttca agaagagcct gataaaaaac agaatataaa gggtgctatg
     7561 agtataagaa tttacataaa tactataaag caggcctttg atgctatatc aaatacagga
     7621 attgatgcta agtataatga aatagataag ggagattata tggaagttgt tgtaaaaatc
     7681 cctaaaaaat agagattact gcttaccagt aatctttttt tgtaatataa gccttgtaat
     7741 ataaattgat ttatagacat tatttatact taaaaaacac ctattcaaca tttaaaatta
     7801 atttaattct taaagggggg aacatcatgg gcaaagttat agctgtattc aatcaaaagg
     7861 gtggagtcgg gaaaactaca acaaatgtaa atttaagtgc tagcttagga actttgggaa
     7921 agaaaatatt agtattagat ttagacccac aaggaaatac aactagtgga tatggtatta
     7981 ataaaaacga agtagaaaat acaatctatg aaattatgtt agatgggtta catattaaag
     8041 aagcaataat aagtactgaa tttgagaatg tagatgtagt tccatcagct actgaactat
     8101 caggtgcaga gatagagctt acatctaaaa ctaatagaga gtatatttta aaaaattcca
     8161 taaaagcggt aatagatgaa tatgattata tatttttaga ttgtccaccc tcactgggga
     8221 tgcttacaat aaattgttta actgctgtag atagtgtttt aattccaata caatgtgaat
     8281 attatgcttt agaaggtgtt agtcaattaa tggagactat aaaattagta aaatcaagat
     8341 taaatgctga tatagaaatt caaggtgttg ttttaagtat gtttgatgga agagctaatt
     8401 tatcaataca agtagttgaa gaagttaaga aatactttaa aggtagtgta tatacaactt
     8461 taattccaag aaatgtaaga cttgcagaag ctccaagtca tggaaaacct gtaatttatt
     8521 atgataaaag atgtagaggg tctgtagctt atttggagct agcagaagaa tttatagatt
     8581 tagaagagga ggaatggtag tatggagaat aagtctaaaa gaagtaatag attaggtaga
     8641 ggtcttagtg cattgatacc agaaattaaa ggagaaactt cagaaaaaga aatagtcaac
     8701 atagatatag acaaaatata tccaaacgaa gttcaaccta gaaagcaatt tgatgaagaa
     8761 aagataaaag tattgtcgga ttctataaaa aattatggtg ttttacagcc aatagtggtt
     8821 aaaatggatg aaaataataa atatatgata atagctggag aaagaagatt tagagcatca
     8881 aaacttgcaa ataaaaatca aatacctgct ataattaaag atatagatat gaaagatata
     8941 atggagatag ccttaattga aaacttacag agggaagatt taaattcaat agaagaggct
     9001 ctagcatata agagtttaat agagcattac aatgtaactc aggaagaaat atcagaagct
     9061 gttgggaaga gtagacctca tataactaat acactaagac ttttaaatct tggacaagat
     9121 gttatagaaa tgatagacag tggtagaatc acagcaggcc atggtaaggc tttattgaga
     9181 attgcagata aagatttgca acttcaaata gcgaaaaaaa tagaagaaga agaactttct
     9241 gtaagagaag tcgaaaatat agctaaaaaa atatctgaaa ataaacaaga agaacctaaa
     9301 aaatctaaac ctaaggatgt atttatttta gatgttgaag ataaacttag aaatatattt
     9361 ggaacaaagg tgaatatttc taaaggaaag aaaaaaggta aaatagagat agaatactat
     9421 aatgatgatg atttaaatag tattgtatca atgcttcttg aatagaacaa aaatataaaa
     9481 cagagaggaa gattgttatg atttatttag ataatgctgc aactacatat ccaaaaccag
     9541 aaagagtgta taatgctgtt ttagattgta tgaaaaatta ttgtgcaaat ccaggtagag
     9601 caggccataa acttgctatg agagctgcta gggagatata tgatacaaga gagaatatag
     9661 ctaagttgtt caatgtaagt aatcctatga atatagtatt tacatctaat gcaacagatt
     9721 ctttaaattt agcgataaaa ggtgtattac aagaaggaga ccatgtaata acaacgtcta
     9781 tggagcataa ttcagtaata agacctataa aagcattgga aaaaaggggt attgaaaata
     9841 ctgtagtaaa atgcgattat gagggatttt tagattatga agatttagaa aaatcaataa
     9901 agaataatac taaattgatt gtaacaacac atgcgtctaa tgtatgtgga actttaatag
     9961 atataaaaaa agttggagaa atagctaaaa aacataatat actattttta gtagatgcat
    10021 cacaaacagc aggtgtttat gatatagacg taaatgaatg taatatagat atgttggcaa
    10081 tgccaggtca taaatgctta tttggacctc aaggaacagg tatattatat gtaagagaag
    10141 ggttgaactt aaatatatta aaagaaggtg gaacaggtag taaatcagaa gaaatagttc
    10201 aaccagagtt attcccagat aaatatgaat caggaactca taatactcca ggtatagcag
    10261 gtcttaacca aggtatacta tttatatttg aaagaggaat caacaatata agacaacatg
    10321 aagaagaatt atgtcaatat atgatagaca aattagagga agtaccagat ataaagatat
    10381 atggaccaaa ggacagtaaa aaaagagcat cagtaatagc tctaaatata ggagatatgg
    10441 attctggaga ggttactttc ttattagata gtgattataa tatagcgact agatcaggaa
    10501 tacattgttc accactagca cataccactt taggtacttt aaaacaaggt gcagtaaggt
    10561 tcagtatagg atatttcaac acaaaagatg agatagataa agcagtggaa gctttgaaaa
    10621 aaatttcaaa aaataaatag catgagattt aataatatag aaataaaaaa aggggatatt
    10681 tcaaaataaa aatttgaatc atctcctttt ttgtacaaaa aagaaaaggg gttcctaaga
    10741 acctctaatc tatttgataa tagtttcaag aaattacttt aagtaatctc cttcttcaag
    10801 aactatatga ccactttcgt aattagaacc atcaacagta atttttattt gttcaacatt
    10861 catatttttt atgtaagtac gagcaactga atcaagcatt aaagtttctg catcactacc
    10921 taagtttgac tttgtaaagt cagagctaac atctaatata ccagttttta caccatcaat
    10981 gtcttttgta gtaaatgaat ttaattttgc agattctgga acaactttaa gtttttgaag
    11041 ttcttcaaat aaagtgttct catcaatttt atttaaatca actttatttt caattaaatc
    11101 tgtcttatct acatcaaaag agtaaatagt agctgattga gctttagttt cttcttttgg
    11161 agtaggttta gctttgttat taggaacctc tttttcagta ttgttttctt cttttggttg
    11221 ctcaacattt gtattgctct tagtttcttt tttagatgaa tcacttccat tttgagcatt
    11281 agtacaacca actgagaata tagatataga taatatactt aacactaaca ttattttctt
    11341 atttttcata aaaatttctc ctctcttaaa aactattctt atatataatt atagtcgtta
    11401 tgagcaaata tatgaataca aaatagtaac aaatttaaaa aatgatttat ttaaattaat
    11461 aaaactcaaa ataaactaaa ttttagtcat ttttatggga aatcatttaa tgtacagtga
    11521 aaatattgta aaataaaagt agtgttttta aaataaatat aagttataat tatattataa
    11581 aaaaatgtct tcaaaaggtg ggaatagtat ggaaattttg agcttaggag aaaagattaa
    11641 aaaattaaga aaagaaaaaa acatgacatt aaaagaatta gctggagata ggataacagc
    11701 agcacaaata agccatatag agagggataa atctcatact agttatgaac tgttggaata
    11761 tctaggcgat aagctagatg taagtgtaga atacttatta gagaccaagg aaatgcaatc
    11821 aaaaaagata actgataatt taatactaca gagcgaaatt tatataaaag aaaatgaatt
    11881 aaacaaggct gaggaccaaa taaaagaagt tttagatata tgtaagagat acgatttaat
    11941 ggagaactat ggcagatgta attttttaat ggctaatatt aatttgaaaa aatctaatta
    12001 taatgatgct atagaaaact ttgaaaaggc attgtacttc tttataaaaa atagtgatag
    12061 tgaaaatata cttaagtgtt atttaaacat agggaaaata tatatgcagg aagactttta
    12121 taaaggtgct ataagccact ttaattttgc agaggaaatt ttgtcagaga ataaatttga
    12181 ggatgttaac atacacaagg aactatatag taagatggca tattgttaca taaaactaga
    12241 taatccaaat atgtcattga agtacataga aaagataaat gaaatagata atagaaatga
    12301 tagaaaagaa gatgtcgata ttttggtgtt aaaagcaaat aatatgcttg agattggaaa
    12361 atatgaagaa tcaagagatt actttaaaaa agcactaaaa atacttgaga gtgaggataa
    12421 taaaactgga ttagcaaatg tgtatttaac aatttgtgat gtttacagaa aaataggtga
    12481 aacagataga gtacttgaat attcacaaaa ggtatatgac ataaagaaaa atgatgaaga
    12541 tgaatatatg atgtcaggat tatttaagat aatagaagca tatatagata aagaagatta
    12601 tgatttagct agaaaatatt gtaagatagc cttagcttca tcaataaaaa ataaaaataa
    12661 attcaatgaa tataaagcat tgagattcta tgctaatatg tataaaaatc agaatgaagt
    12721 aactcttgca attgaatatc tgattaaatg cataaaaata gtatcagatt taggcaacaa
    12781 tagaatattg gccaatttat atatagactt aggtcaatta tattcaaata tatcaaaaga
    12841 aaaagagtta gaatattatc aaaaaggtgt atttatgtac aaaaatttag aaataatgta
    12901 gggtaaataa acctataagg gtatagaaac aatctataac aagcaagata atatctatga
    12961 taaaatataa attgtatgaa tatttttaag gagatgaatc agacaatgag tataaaaata
    13021 gatgcgagag ggcttgcttg tcctaaacca gttataaata caaaaagaga attggataac
    13081 ctagaagaag gtgttgtagt tacaatagtt gataatgaaa cagctaagga gaatatatta
    13141 aaacttgcta aatctttaag ttgtgaagca aatattgttg acgaaaaaga gaattttata
    13201 tctatagaaa ttagaaaagg taaaaatata ctagataaac aaaatgttgt agataataaa
    13261 gaaagtgaat tagacaatac atgtatattt atatcttcag ataagatggg acttggaaat
    13321 gacgaattag gaaaagtgtt aataaaagga tttatatata cattaacaga atctaaacct
    13381 tatcctaagt atgtactttt agttaatgga ggagtaaaat taagtgctga aaatgaagaa
    13441 accatagaaa atctaaaaat acttgagaat atgggtgtag aagttttatc ttgtggtact
    13501 tgtttagatt attataattt aaaagacaaa ttacaagtag gtacagttac aaatatgtat
    13561 actatagttg agacattaaa gaatgcatca aataccattt ctatataatg tatgtatgtt
    13621 taataatata aaactaacaa taaagattaa ctcaaataga tagtcagaaa ggattaagta
    13681 atgaatcaaa tgtatatagt gtcatttaat tcgacacatc atgcgataag atcagaaaag
    13741 ttatttggtg aaaatagtct aaaagtaatg gctcttccaa caccaagaga aataacagca
    13801 agttgtggaa tatcaattaa attttctttt gaagatatgg aaaagataaa aactattttg
    13861 gttgaaaata atgtagatat aaaaggaatt tattgtataa gtaaattaga aaatggttca
    13921 aaagaagtag agaagttaga ttaggaggct aattatttat gccaatggat ttaaaaatag
    13981 gtgatattgt tgaattaaaa aagcaacatg catgtgggtg taaagagttt gaaatcataa
    14041 gaacgggaat ggatataaag ataaaatgta ctaaatgttc aaggctaata atgcttgata
    14101 gagagacctt agaaaagaga gtaaaaaagc taataaaaaa gtgataaaaa tttaacactt
    14161 aaaaaatgtc aaataccgta tattaatatc caaaattttg gtataataag aattgaagaa
    14221 aacattttca tgggggtgct ttaattatgg cagttaaata tgcaaaaaga atgcaaggat
    14281 tacaaggatc agaaatacgt gagcttttaa aacttactca acaaccacaa ataatatctt
    14341 ttgctggtgg aatgccggct ccagagttat ttccagttga ggaaatgaaa aaagtatcag
    14401 ttgcagtgct tgaagaaaat ggaagatcag ctatgcaata tactacaaca gaaggatatg
    14461 aaccattaag agaaaaaata gcagctagaa tgaatgataa aaacaaaact aatgtaaaca
    14521 aagatgatat attagttaca agtggttctc aacaaggtct agattttgca ggaaaagtat
    14581 tcattgatga gggtgatgta attttatgtg aaagtccatc ttacatagga gctataaacg
    14641 catttaaatc ttatcaacct aaattcatag atgttccaac agattctgat ggaatgataa
    14701 tggaagaatt agaaaagata ctagaaacaa ctgatagaat aaaaatgatt tacgtaatac
    14761 ctgacttcca aaatccaaca ggaagaactt ggccacttga aagacgtaaa aaattcatgg
    14821 aaatagtaaa taaatttgaa ataccagtaa tagaagataa tccatatgga gatttaagat
    14881 ttgaaggaga gactttacca tctttaaaat caatggatac aaaaggatta gtaatattct
    14941 taggaacttt ctctaaaata ttctgcccag gatacagatt aggatggact tgtgcttctc
    15001 cagaaatttt atctaagttt aactttgcaa aacaaggtgc agacttacaa gcatcaacta
    15061 tatctcaaat ggaagtaagc aaatttatgg atatgtatga cctagatgca catgttgaca
    15121 aaataaaagc agtttatgtt aaacgtagag atgtaatgct taagactatg gaagaagagt
    15181 tcccagaagg attagtattt actcatccag aaggtggatt atttacatgg gtagagcttc
    15241 caagtaattt aaatgctaaa gaattaatgc caaaatgttt agataagaat gttgcttatg
    15301 ttgctggagg aggattcttc cctaatggtg gtagagaaaa tactttcaga cttaactatt
    15361 caaatatgcc agaagaaaaa ataatagaag gtataaaaaa tatagcagct gtattaaaag
    15421 aagctatggg tgtagaagct taattaaaaa taataactta tatataatac aagggatggt
    15481 tgttgccatc ccttgtttgt ttgaaaagta tataattcac cttatactaa actttgtcca
    15541 aaatttaaat tgacagaaat aagaataaat gataaaatgt ataggtatga gattttagtc
    15601 tcatttctct gctctaagag tgtagagccg ttaagtccaa agggaggtga attatagtga
    15661 gaaattatga attagtttat gtggtaaagc caaactctga tgaagaagta agagaggcta
    15721 tactaaacaa agttaaggaa gttgttgcaa ctgacggaga aatagttaaa gttgatactt
    15781 ggggaactaa aaaattagct tatccaatag ctaagtttac agaaggtttc tatgtattag
    15841 ttaacttcaa atcagcagta gacgttccta aagagataga cagaaactta aagataaatg
    15901 aaaacgtaat aagacacatg atagttgttg cttaataact ggttttatta taaaaaatag
    15961 gtggtgtttt tatgaatcaa gttgtattag tgggaaggtt gactagagac cctgaattaa
    16021 gatacatccc aggtacagga acaccagtgg catcctttac aatagccatt gatagagact
    16081 atgtaaaaaa agatggatct aaagaaactg attttatacc tgttgaagta atgggtaaat
    16141 ctgctgaatt ttgtgctaac tacataacta aaggtagatt agtagcactt caaggatcta
    16201 taagagtaga caattatcag acacaatcag gtgaaaagag aacttttaca aaagtaagta
    16261 ctagatcagt acaagctttg gatagtaaga ataaatcgga aaattcatat agagagagtg
    16321 ctccagcttt tgagccaagc tttgaacctc aaggtttaga cccacaaggt ttccaagcta
    16381 tagatgatga cgatatacca ttttaaaaag gagggaaaaa agtcatgatg aataagaaaa
    16441 gacgtaagaa aaaaagagtt tgtcaatttt gtgctgacaa aaacgctaaa atagattaca
    16501 aaagtactca aagattacaa aagtatataa ctgaaagagg aaagatatta ccaagaagaa
    16561 tatctggaac ttgtgctaag catcaaagag aattaacagt tgctataaaa agagcgagaa
    16621 atatagcact tttaccatat acattagact aaaaaaatga gccttgtgct cattttttat
    16681 tatatgtatt tatatatcta aatgaggcga gctataaagc tcgttttttt gtttatatac
    16741 ttaaatatat tattatttat gatataatat tatatattaa ggagggttgt aaacgtgaaa
    16801 atagataaag gttctgagat aacgagaaac ataaagataa tagaatggat gaaaacagaa
    16861 atattaatga gtgtaagtga tttgtttaat ttattgttta aaggggtaaa accattagat
    16921 gaagcaattc aagatacttt agcaaatata ataatgataa catatctctt agcaaagaga
    16981 ttaggaatta gttttagaga tgtggattat aaagtcaagg aaaaaataaa aattggtata
    17041 gatgaggacc atagcgttga aagatggtat ggagatttat ctaatttaaa aaaacatatg
    17101 gataataggg agtgatatta aattgaataa taaaattaga ctatctaagg caatgtctat
    17161 aatagtttta gctataataa tcgctttagt aatagcctat attccaatgc taggaatatt
    17221 tagtatgtta gcagctgttc catatgtaat tataggtgct attactgata gaaagtattc
    17281 tcttatatct attttggtta cattttgtgt actgattcta tttgcagaca tttcttatgc
    17341 attaaatata tgtataatgt attctttacc aggtatagta ataggaaaga tgctaaagag
    17401 aagctttgaa caagaggaca gtaataaatt cgaaccgata tatggtggaa ccataatatt
    17461 tgtactttca atgcttgtat atttttttgt attaaagaca tttttaaatg tgaatctatt
    17521 agatgaagtt tctaagatga tatcagaaat tgtaaatatt caaaaaaata gtttttctgc
    17581 aactgaactt aaagtgtttg ataaaatgaa gccagatgag attgtaagtt attttacaaa
    17641 tatgctacca atgatgttat ttttacaagg gttgttatca gcgtttgtca catattattt
    17701 gagtgtattt tttataaaaa ggattacaaa gatgaatata agatttccta agtttgctga
    17761 tttttattta ccgggaaatg ctatactcac aactttctta ctttatttat tagtcttatt
    17821 tattcaaatt attggttcaa aattatatac agaattaata atgactaatt tacagctagt
    17881 ttttaattta atgtttgtaa ttcaaggtat agcagtttgc atttatttct taaagaaatg
    17941 gataagacag ggaccaaata aaattatgtt tttttcaggg ataattttat gtttatttgg
    18001 atttatggga atatcttttg ttggtatggt agatagtata attgacttta gaaaggtaag
    18061 aagctataaa tccacttagg aggatagaaa tgagtaataa acaaaccttt aaattaaata
    18121 tgccagaaat aaatttatat ataattgtta ttggtatttc tagtataatt ttgctttatt
    18181 acaacttgta tgtagggtgt ttatttttct gtatttttgt atatatggtt tttcacaatt
    18241 ggagaactac taatattaga cgtcatgaat ggacagagta tattcaaaat ttatctttag
    18301 atatagatga aacaactaaa aaagccataa taaatcttcc aataccatta tgtattttag
    18361 aatttgatgg aaatatatcc tggtataatg gaaagtttta tgatatgatt ggtcaaaaag
    18421 atttacttga taaaaatata gaggatatag ttaaaaatct taatttaaga aaagtattaa
    18481 atgaaaataa agagatgtac acagaaataa actataaaga aaaagaatat acaataattt
    18541 ataatgttat aaaaaatgat caagaaaaaa atcctaaata tttgatgata ttatactgga
    18601 tagacaagac tgaatattta aaagtaaaac aaaattatga tgatgaaaaa aatgcaatga
    18661 tgctaataca agtagatgga tatgatgaag tattaaaaag tgcagcagaa gataagagag
    18721 ctttaattaa tgttgaagta gagaaaatac tttctgcttt agaattaaat tcaaatggtg
    18781 cattgagaag aacttcaaaa gacaaattct tcttggtaat gcataaaaaa gagttaaaaa
    18841 aattggaagc tgagaagttt tctattttag atacaataag acatattgat tatggaaata
    18901 atcttcctgt tactataagt ataggaattg gtatagatgg agatacatta aatgagaacc
    18961 taaaacttgc tacgggtgct ctcgatttag cacttggtag aggtggtgac caggctgttg
    19021 taaaaacaaa agataaattt gtgttttatg gaggaaaatc taaagctgta gaaaagaaaa
    19081 ccaaagtaaa atccagatta ataggtcatg ctttaagaga ggttatacaa caaagtgacc
    19141 aagtttatat aatgggacat aaatatcctg atatggatgc tatgggagca gcagttggtg
    19201 tttatgatat atgtaaatcc tgtaacaaaa ctgcaaacat agttttacaa tctgtaaatg
    19261 aatctataga aatatttata aataaaataa atgaaaataa ttactataaa aaattgttta
    19321 ttggaaaaga ggaagctatt gacaactgca ctaaaaatac attagttgta gtagttgata
    19381 ctcatagacc aaattatact gaatgtgaag aactgttaaa gctatcagag aaggtagtag
    19441 ttatagacca tcatagaaga ggtgtagagt ttataaatga tgcagtgctt ttattccatg
    19501 agatatatgt atcctcaacg tgtgaaatgg ttacagagtt agttcagtat atggatgaag
    19561 atgtaacaat aaataaactt acagctgaag ggcttttagc aggtataagt ctagatacta
    19621 aaaactttgc attcaaaaca ggtgttagaa cttttgaagc tgcttcttac cttagaaaag
    19681 ttggagcaga tactatagag gtaaagaaat tcttcaattc tgatgtaaaa gatttcataa
    19741 taaaagctga aataattcaa agtactaaaa taattaataa tagaatatgt ttagcatatt
    19801 caagtactga gatagatagt ataaatgtta taatagctca aactgctgat gagttattaa
    19861 atatcaaaga agtagaagct tcctttgtat taggtgaaaa agatgatact atatttataa
    19921 gtgcgaggtc tttaggacaa ataaatgtac atgtacttat ggaaaaatta ggaggcgggg
    19981 gacacataga tatagctgga gcacagttaa aaaatgtttc tctaaaagaa gcttataaga
    20041 tggtaaataa aataatagag gagtatttgg aggaggaaga atagatgaaa gttatattat
    20101 taaaagacgt aaaaggaact ggtaaaaagg gagaaatgaa agaagtaagt gatggatatg
    20161 caagaaattt cttatttcct aaaaagatgg ctgtgcaagc agatagtgta gcaataaaag
    20221 aattaaatga aaagaataaa tctaaagaaa taaaagcaca aaaagagtat gaggaagcag
    20281 ttttattagg taagcaaatg gaagaaatta atatagaaat atattcaaaa gcaggagaag
    20341 gtggaagatt atttggttca ataacttcta aagaaattgc tgagcaatta aagaaacaaa
    20401 aagacataga tgtagataaa agaaaaatat tattagatga accaataaga tctttgggtt
    20461 caacttttgt agaaataaaa atacatcaaa aagtaactac taaaataaga gtggatgtaa
    20521 aagaaaaaca gtaataacta gaggtgatta agatggaaga tatgacgaga attcctcctc
    20581 atagtgtaga atcagaacaa tcaatattgg gttctatact tctagataaa gatgctataa
    20641 taacggttac agaaactata aagcctgatg atttctataa agaggctcac aaaataatat
    20701 atgaatgtat gataacttta agtaataaag gtgagcctat agacttgata acattaacag
    20761 aagaactaag aaaacaaggg catcttaatg atattggagg aatcagttat attacaagtc
    20821 tttctactat agtaccgacg acttcaaatg taaaatacta tgctggtata gtaaaagaaa
    20881 agtctgtact tagaaagctt ataaaagctt ctaatgaaat aataaatcta ggatatagtg
    20941 gagcaactaa gattgaagat gttttagaac aagctgaaaa gagtatattt gatatatcac
    21001 aggagaaaac tagtgatgat tttaaatcta taaacttagt tttgatggat gcctatgata
    21061 tgatagaaaa attatacact aacaagagtg atgtcactgg tataactact ggttttaaag
    21121 atttaaacaa aaaaataaat ggtcttcaaa ggactgattt aatattaatt gcagctagac
    21181 cagcaatggg taaaactgcc ttttctttaa acttggttca aaatgcagcc ttaaaaggtg
    21241 atgcctctgt agctgtattt agtttagaga tgtctaagga acaattagta caacgtatgt
    21301 tatcttcaca atctagtgta gaattaaaaa aaataaagac tggaacactt aatgataatg
    21361 attggcctag aattatagat gctatggcag tattatcaga tgccaaaata cacatagatg
    21421 atactcctgg tataaaaata tcagaactaa ggtcaaagtg tagaaagtta aaaatagaaa
    21481 aaggactaga tttagtgctt attgattatc ttcaacttat ggaaggtgaa ggtaataatg
    21541 aaagcagaca acaagagata tctaagatat caaggtcact aaaaatatta gcaaaagaat
    21601 taaactgtcc tgtagtagca ctatctcagt tatcacgtgc accagaacaa agagcagacc
    21661 atagacctat gttatctgat ttaagagagt ctggagcaat agaacaggat gcagatatag
    21721 ttatgttctt atacagggac gaatattatc atgctgattc tgagagtaaa aatataggag
    21781 aagtaataat agcaaaaaat agacacggtg aaactggttc tgtggaactt gtttggcttg
    21841 gtgaagttca aagatttggg gataagttga gagacctata atctaacgaa tatccacaga
    21901 acgtcctttc tttctacagg aaaaatgtgg atacatgtca ctaaatatgt ccaaacattg
    21961 aaatttagct aaaaaactat ccacactatc cacagatggc ctgttgataa gtgtgaataa
    22021 gtagataaag caagcttttg gcttgcttta tctgtgtata aatattaatt attaggagga
    22081 ataatatgga aaaaataagc ttaaaatata tatatcccaa tataattaaa gtacttgatg
    22141 aaataaactt atttagagta attgacaata atttaagaga gtctattgtt gtttatgcta
    22201 ataatgtgga taatcagtat catataaaca tgactaatac aaactttgga aacataatta
    22261 acatatgtaa attagaaaaa cttttagatg tggataaatt tatggaaaaa gttataaaat
    22321 atgaaaaaga aataatagag aaagaagagt tttcaaaaat agaagaatat atgttaaata
    22381 ttggagaata ctaaaaaagt tgaaaaaaat acgaatgttt gatagaataa gtaagtaaag
    22441 tgttcgagag tatataaaat atagaggtga attacatgaa aacagttgca attgttggtt
    22501 ctcaatgggg agatgaaggt aaaggtaaag ttatagatta tcttgctact caagcagatg
    22561 tagtagtaag agggcaaggt ggaaataatg caggacatac attagttgta gaaggtaaaa
    22621 aatacgcatt acatctaatt ccatctggag ttttaaatcc aaatacagtg aatataatcg
    22681 gaaatggaat agtgtttgac cctaaaggat ttttagaaga attggaaatg tttaaaacgg
    22741 ataatataag tacagaaaat ataaaaataa gtgatagagc acatgtcata ttcccatatc
    22801 ataaagaact agatgcattg tcagaagaag ccagaggcga tttaaagata ggtacaacaa
    22861 aaaaaggtat tggaccttgt tatatggata aaacagagag atctggtatt agaatatgtg
    22921 acttaatgga taaagataaa tttgctataa aattaaaagc tcaaatagat gctaaaaatg
    22981 aaatagttaa aaatatatat ggaaaagaag aattatttga ttttgaaact atatataacg
    23041 aatatctagg atatgcagaa caaataagaa aatatgtggc agatacgtct gttattgtat
    23101 atgatgcagt aagagcaggt aaaaaagtat tgtttgaagg agcacaaggg actttattag
    23161 atttagattt aggaacttat ccatttgtta catcttctca tccaacatct ggtggatttg
    23221 caattggtgc aggtatagga ccaaatatga taaaagacgt tgttggtata gtaaaagcat
    23281 atactacaag agttggagaa ggaccgtttg ttacagagca aataaatgaa actggagata
    23341 agataagaga acaaggacat gaatttgggg taactactgg tagaccaaga agatgtgggt
    23401 ggtttgatgc agttatagtt aagtatgcag ctagagttaa tggattgact agtatttcat
    23461 ttatgttact tgatgtatta actggatttg ataagataaa agtttgtacg tcttataaaa
    23521 tgggcgataa gataataacg gatttcccag cttcattaga tgatttagca aaatgtgaac
    23581 ctgtatatga agaattagat ggatggaatg aagatataac tcaaatagat aactttgatg
    23641 accttccaga aaatgcaaag aaatatgtag caaaaataga agaactagtt ggagtaagtg
    23701 tagatatggt ttctgttggt ccaaatagag ctcaaactat aataagaaga aatatatttg
    23761 cttaatattt tatcaattaa aaaatttaaa atagttgaag aagagtatct caagataatg
    23821 tttatagtta ttagaggtgc tcttttatat ttgacttaca ttgatttttt attaagttaa
    23881 agctacttat acatattcta actgaaatta aagttgaatt tatgtagata catataggtt
    23941 ttttaaaaag aattttgttg aaaataaatg gctaaagatg attttaatta tattctaaaa
    24001 aaagtaaaaa aaactattga ctaaaaatta tatagacgct ataatataac ttgtgagtga
    24061 aagaaacgac gaaaaaagtc gaaaaaatgt ggtctattag ctcagtcggt agagcacctg
    24121 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaat atggctcgtt
    24181 ggtcaagagg ttaagacacc gccctttcac ggcggtaaca ggggttcaat tcccctacga
    24241 gtcaccagta tgggactata gctcagctgg gagagcacct gccttacaag cagggggtca
    24301 caggttcgag ccctgttagt cccaccaaat tttgaattag agataaatac tagattatat
    24361 gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta ggttcgattc
    24421 ctatcaccag ctccacaaag tacataaatt caaattaata taattttcta tgttacagga
    24481 tacttttaag tatctttttt taatttcatt tttattgtat taaaaatgaa attaaacata
    24541 caacaaaatt ttgaattact tgcaatgtga tagttttaaa tataaaattt agaatcattc
    24601 tagcatagta ttatgtaata tagcaaattt aatttatagt atcaataaat atagactact
    24661 aagaatgtaa atgatttgtt agattaagat acattagata tgtaaataga aaaataaaaa
    24721 gtatgtttta atttatgtga tataagtttt aaacagagaa gcaagtttgc tcttacctct
    24781 ctatttaaaa ataaaaaaat gttttttaag ggctatttta tttaaatctt tcccatctca
    24841 aatcatctcc agtaaattct aaaatcctaa acttatctaa tattctagag agagttcttt
    24901 gagtgtaagt atttcctatt tgagatggtg ttaagttggt tgaaataatg attttcttac
    24961 cagcaacaag tcttgtattt acaatattga agatttctcc actagtaaaa gaattattaa
    25021 gttcagttcc aaggtcatct attattaata aatcacaatc aaacaagttt ttataattat
    25081 cttcacttat ttgattattt gtgtctcttc taaatttata atcttcaaga atatctaaaa
    25141 ttctaaaaga agtttgatag attacaacat tgcctttatc taacaattcc tttgcaatac
    25201 aattgcacat ataggtcttt cctagtccag tagaaccata aaacagtaga ttttcactat
    25261 tatccttctt aaaatcatga acaaaatttt cgcagatact taaattattt agcatatttt
    25321 ctctaggaga tatttcacca tcagagcctt tcttaggaga aaatatattc aagttaaaat
    25381 tcgaaaaatt ttcctgactt aaaatcctat ctaaattaga cattttataa gcttcattta
    25441 caatttcttg tttgaggcaa gaacattttt ctccattagg taaaaatcct ttatctttac
    25501 aagaaataca ttgaaacttt atttccaagt aatcaagagg tatgttactt tcagctaaaa
    25561 gtctttcttt ctttactttt aaagactcta tattctcttt agtcttcttt acaatttcat
    25621 ctttagactt gggatttaaa agtactatct tagctaaact gagacctatt ttagaaattt
    25681 catcatctat agattttatc tcagggattc tattatatac ctcatttttt ctatgttcta
    25741 ataagagttc attatcatct cttctcttag catatttagc aagtattttt cttattttat
    25801 cctcattcat ttaatcacct acttaaactt ttctttttga ctttttttaa taatcttatc
    25861 gagctcatca gaggtatact gagtgaatgt ttcgttgaaa ttatgaaact tagttttttt
    25921 gtaagtattg ttgttttgag tagtattaat ctgcttctta ttaatattct ccttaactat
    25981 acgttcttcc tctttttgct ttaaatcatt taaatttttt atattttttt cattccaatt
    26041 tttaattata ccatttatat aagatatact tgggtttgaa atattcttag acttagaaca
    26101 tgcatttatt atcaaatcta tatccatatt aaatttatca aaccaggtgt ccataagttc
    26161 tttttctgat tttgatggtt gtcttgaaaa tcccaattca ttgaaaatcg ttttatataa
    26221 aatatatcgt tcacttctta ccaaaaagct ttcttcaaca tcttttggtg tatataaatt
    26281 tgaatcatac caatttctaa taatgccttc aatgtattta acgggctttg atgtaccagt
    26341 tttatctttg acgtattcat atgcgcaaag tatcatatcg gggctcatat tgtatttatt
    26401 cattatatcc ataatactca tcttttcact tgggtcgaga tatcgcccaa ctattttatt
    26461 tatagaatta aacatttttc taatacttgg attttcagaa gtagatacaa ccttatctgt
    26521 attagatttt atagatgaat tattgcttaa tatattctca atatagaaat tttttaaatc
    26581 taaaaattct attgaataat taaaattatc atattctcca ttgtcatgta tttttataat
    26641 cttcttttct tcccagaatt tccaagcaga taacacatca gacaatggta tatttaggtt
    26701 tttggctatt gaattattgt cgaacttagg attagaagta atatcacatg cttgtctata
    26761 tcctaaaaga taaactttta cgtataaccc gtccgccatt ggcataaata tatctataaa
    26821 gatattggga atagtgattt cacctaaatc tatttcattg ctttctttaa aaaacatttt
    26881 atcacctcag attaaattat atcatttttt atattatatt tgtctgtaaa caaataaaag
    26941 gtcacaagat tagcatgtaa atattttcta aaagtattca ttttgaaaag gaggagatat
    27001 tttggttata aaattcaacc aaaaagtaag aagaacatta tcagtagcga ttctatttat
    27061 ggtattaata ataggttgct ttatagaaaa ggatgacagt ttagaaacat acaaagaaaa
    27121 aactaattca aaaataaatc agtataatat ggatgttata tttgatgatg agactaagag
    27181 gttaatgtgc aatcagaatg ttgactacat aaataataca aaatcaaaca tagataaaat
    27241 atatttccat atatatccga atgctttttc caagaaagat tttgctcctt ttgaaaaaag
    27301 tgaaatgaca agggcttatc ctaatggatt taacgaagga tatatagata taaagaatat
    27361 attaaataat aatagtaaaa tggagtataa aattaaagga gacaaaaatg acatattaga
    27421 aatagaatta gggaaagagt taaaaccaaa tgaaagaatg tcattagata taaaatataa
    27481 cgtaaaaata ccaaattcaa ttggaagatt tggatatgga gaaaatacta taaatgttac
    27541 taattggttt cctatagctt gtgtaaatga tgaaagggga tggaatttaa agagttatga
    27601 aactattggc gacccatttt atagtgagac tagcgatttt catgtgaaac tattgattcc
    27661 aaataaatat aaaattgctc atacaggaaa acttattgat gataaacatg ataataagaa
    27721 aatgctatat gaaatagaag ctgaaaacgt tagagatttt gcatttatac taagtagcaa
    27781 atttgatgta aacaaagtag attcaaaggg agtagctatc aatacatata atttaaataa
    27841 aaagctttct aaaaaagcaa ctgaagtggc aaaagattca ataaatatat ttagtgaatt
    27901 atttggaaag tatccttaca aagaattttc tgttgtagca agtgactttt ttataggggg
    27961 gatggaatac cctatgcttg ttatgataga ccaaagttta tataatgaaa aaaatgaatt
    28021 tttactagaa tatgttattg cccatgagac agcacatcaa tggtggtatt ctgctgttgg
    28081 aaatgatgaa ataagtgaac cctggttaga tgaagcactt actgaatatt ctacaatagt
    28141 ttattttgaa caaaagtatg gtaaagatat ggcaaacaag cttataaaaa ctatggagat
    28201 acagacaaaa agttatttga gcgaaaatat atttaagcca gcaaatgagt ataaaaattc
    28261 tactgaatac agcttgaatg tttatacaaa aggcgcaata gcatttaatg aaataagaaa
    28321 agaagttgga gataaagtat tctttgagac tttaaatgaa tactataata agtataagca
    28381 taaaaatgct aatggaagag cttttgtgga actatggaat agtaaaggtg tagatataaa
    28441 taagattata agtgaatgta agtaaacagt gaatgtaagt aaattttgtg cagagtatta
    28501 aattttattt aatactctgt ttttttggta taatataatt tataaggcag aaaaagaggt
    28561 gaaggaatgc taaagtattg ttcagtaggg agtggtagta gtggtaattg ccactatata
    28621 gggtataaaa atactaatat actagtagat gcaggattaa gtggcaagag aataactaca
    28681 ggtcttaaag acattggtgt agatgcagat aaattaaaag gaatatttat aacacatgag
    28741 catgttgacc atataaaagg agcaggtata ttatcaagaa agtttgatat accagtattt
    28801 gctaatataa aaacatggtg ttctatgaag gacaaattag gtgacatcaa agataaaaat
    28861 atgaaagtat ttgaaaatga taaaacctac tcattaggtg atatcatagt aagacctttt
    28921 tcaatagaac atgatgcgtc tgacccagtt ggatataatt tttatacaga gagtgatgaa
    28981 aaaatgtcta tagctactga tataggatgt ataactcaaa atataaaaaa acatctatat
    29041 aaatctaaat tagtagtatt ggaatcaaat tatgacccta atatgcttat gatgggttca
    29101 tatacatatg ctttaaagaa aagagtaatg tcaagtactg gtcatttatc caatgaagat
    29161 gcagctaatt tctgcgttga acttattaat gaaggtacag aatctatatt attagcacat
    29221 ttgagtaaag aaaataattt tcctgagtta gcgtatgaga cttcaaaagg agtattggca
    29281 tcaaatgata tagtagttgg acaagatgta aaactagatg tactatcacg aaatgatatt
    29341 tctaatgttt atgagatgaa gtagaggtga ataactttgc taaagagaaa ttttgaaaat
    29401 gttgaggata ataaaatttt atactctata aagcagggca tgattttagc gatacctgct
    29461 attatgactg gttcaacggc tcttgtaatt ttgaatcttc caataaaggc ttatcaagaa
    29521 tatttatcag gcttgtttaa tggagaggtt acaaatattc ttaactttat aaatgattct
    29581 actttaggaa ttatttcatt aataatatta ttaacaataa gctatagtta tggaaaaata
    29641 tatgggagca agtatacagt acttgttcct atagttgcaa tgtgctcttt tttagtattt
    29701 tcgcatggta atgagcttaa ttcttatata gaaatattta agacaaagtg gttatttaca
    29761 tctataattg tttctatgac atcttctgta ttgtttgtaa aacttacaga atcttttgtt
    29821 acaagtgtaa aatttcatac agaaggggct gatgcagact ttaatatggt agtttcggct
    29881 atagttcctt tcataatagt agtatttttg ttttctattt ttagagtgat aatgataagt
    29941 ctaattggca gttcaaactt tcaagatata ttttataatt tattttcaaa tgtttttaat
    30001 aaaatgggaa caaaccttat gagtgctctg ttgtttattt ttttaatgca ctttatgtgg
    30061 ttttttggga ttcgtggtag taatgtatta gataccgtcg caaaaaattt atttgaaaat
    30121 agtatggcaa ttaacataaa tttagttaat aataatcaat taccaacaga aatttttact
    30181 aagacctttt ttgatacaat ggttttattg ggaggatgtg gctctttact atgtttagta
    30241 attgcaattt ttcttagtga aaaacgtata aatgtaagaa agttagctaa aattgcttca
    30301 ataccagctc tttttaatat caatgagatg ctagttttcg gtataccaat tgtttttaac
    30361 tatattatgt ttgtaccttt tgtaattact cctataatct taaccatcac aagttatgtg
    30421 gctatgtcta caggtattgt accatgtaca gtttcagctg tagaatggac ctcacctata
    30481 tttttaagtg gatatatgtc tacaggttca ataagaggta gtttgttgca agtatttaat
    30541 ttatgtgttg gtgttatgat ttatattccg tttataaaaa tgtcgcaaaa tagatataca
    30601 tatatgttta aaaataatat agaaaattta acaaacttat taaaaaggta tgaattaaca
    30661 ggtgaacagc ctaatcttct aaaccataat ggtaaaatag gttcaatttc aaaaatgttg
    30721 gcatcagact tgaagtttgc tatgaaaaga ggagaaatag agctttttta tcaaccacaa
    30781 gtcaattata atggaaatgt tgtgggagca gagggattac ttagatggaa acattcaata
    30841 ggagggttca tttatccacc actagttata attttggcta aagaagaagg gttcttagat
    30901 gagcttggag agtatattat taataaagca tgtattgatt taaagaagag tgaaaagcta
    30961 ttgagaaatc ctgttaagtt ctctgtaaat atatctccag accaacttga tgaccctaag
    31021 ttaccagagc aaataaaaaa tatcattagt agtaatgata taaatcctaa tatgttaggt
    31081 atagaaataa cagagcaaat tgcattatca ggttcacaaa taattattga acgtatcaat
    31141 gcgatacata acttgggaat aaagttaatt atggatgatt ttgggatggg gcatagctct
    31201 cttatatatc ttcaaaataa taactttgat atagttaaat tggatggttc attagttaaa
    31261 gagatactta caaataaaag aagtagtgaa attataatga ctattgtaaa tctttcaaaa
    31321 aatttagatt ttgatatagt tgttgagtat gttgaaaata tagaccaaag ggataagtta
    31381 tatggtttag gatgtgaaat atatcaagga tattattata gcaaagcaat accttttgaa
    31441 gagtttgtag aatatgcaaa tcaagaaaga gagatataat tttataagag cttgtaaatt
    31501 ctagtaatgc atagatttgt aataaaatta agtttaggag aaatatatga atataagtat
    31561 agtagtagta ggtaaaatta aagaaaaata tttaaaatta ggtattgatg agtttaagaa
    31621 aaggctttct aagtattgta agttagaaat tatagagttg gatgatgaaa aagctccaga
    31681 gaatttgagt gttaaggaaa tggaaattat aaaagacaaa gaaggaaaga aaattttagg
    31741 gaagataaag cataatagtt atgttatagc cttggctatt gatgggaagg gtttatcctc
    31801 tgaagaactt gctaagacta tgagtgattt agcagttaga ggtaatagta gtctctgttt
    31861 tataatagga ggttctctag ggctttctga tgaggtttta ggaagggctg attataggtt
    31921 atctttttct aggatgactt ttcctcatca gatgatgaga ttgatattgt tagaacagat
    31981 ttatagggct tataggataa ataatggaga accgtatcat aaataaatga tacggttttt
    32041 tatgatataa ttgaattata aaataaaatc aaaatgtaaa gtaggtgtag taatggacag
    32101 actagatata tttatcgaaa attatgattt taaagaatat cttgaaaaat tacctagaga
    32161 attaatagaa gaaaattata aagattttgt tatagaattt tggaaaaatc ctagtgtaga
    32221 tattattaaa ctaaaatcaa aatatagtat aaaaaatgta gtatctataa gtagtatatt
    32281 aatgcaaaat cctatccaag agtattatga gtttcttgaa gatatttgga aaaataacat
    32341 aaaaatagat gaattaatta ataagtattt aattacagca acaagaatgg atcaaattaa
    32401 attcccatta atatatattg atgaagattc tttttgtcct tattgtttag gtaataaatt
    32461 caatattatt aataatatta agcaacaaga tataaaagtt gaatgtgaaa agtgtggtga
    32521 ggcaattagt tattttattc ataaggaaga agtagataat ataatagaag aagaaaaaag
    32581 aaaaaaagaa ctatttataa gaacattgga gaatgtatat aataaaataa acaagattaa
    32641 atgtcataga tgccaatcag atttatatgt gtgtattaat gataaaataa tggagtataa
    32701 tattaggtgt gatagatgta attcacagta tgaatcggtt gaaattgcac aggaacaata
    32761 tagattatgg caacaaagag ctgcaatgat ggctaacata aaaaataaag aaaatgaatt
    32821 gttgctaaag acactatcaa ttaaggatga aaaagatatt aaatttaaac tcgaagatat
    32881 aattacggag agtgaaagta tagatgtaat tagtgattta gtagatatgc aagatagagg
    32941 agagaatatt ttcaagcaat tatttaataa ttatgttaga ctaggaagat tagacagaag
    33001 gttattggta actgtgtgta aagattgcaa gcaaaatgaa aatacttata catgggaaag
    33061 aggcaaagaa catttaataa atatgagggg gattacatac agtgaagcag ttgtatatga
    33121 gttaatggaa aaaacaaagc tattgccaat aagagcaatt attagaagac ttatggagaa
    33181 aaaactattg tattgtgatg aaaggagcaa taatttacaa gtacaccctg ttttgattga
    33241 aaatttagct aaactagaat caataaataa atctcaaaat ataaataatc aagtagctta
    33301 tctaatattc aataagcaaa aatatatatg ctatcattgt ggtgaatcag gaagaccctt
    33361 aaagatagga tatctaacaa tagataaaaa tataaatgat ttatcaagtt tagtgggaat
    33421 atgtgatagt tgctatgaag atattactga agatgaaatt attatagata atattttttg
    33481 tctggtagat gaaagaaaca atgaattagg ggaagcttgg aaatttttaa tacaatatga
    33541 tagtagtttt agaaataata caattgcaga agtagagcat gcaagattat atgatatgta
    33601 tgatgaagat aatttaattc gtgcgtatgc aaatacattg tataaacaaa cgaaagatgg
    33661 gtttgctaat gaaaaagtat tttttacata tgcaagagca atattgaaaa atagcaaagg
    33721 gcaagtaagt gtactacaaa tagcaaaaga aaaatttaaa ttagaaaagt ggttaaatgg
    33781 ataaagtgat aatacatatt aaaattataa atttatataa aactgtattg caattaagca
    33841 atatggtttt tatatataag ataatgatac aattaaccaa aatgtggtag tacataagtg
    33901 ttatgagatt taaatattag cgattaaaat aatgattaag taaaattgta attgatgagg
    33961 agtattaacg tattagttta tttttatata taattaaaat ataggaggga agaatgtgag
    34021 tcatagtaaa ctatctgatc acaaattaaa aaaggggaaa tttatagcac cttttaatga
    34081 atttatgaca ccattgaata gtaatgagtc atggtgcctt ggtcgattgc cagagtatat
    34141 atggatagga ttattaataa ataaatatgg tagaaaaaaa gggatggaaa taatttataa
    34201 aataattgta aaacttcatt caattgaatc agatattgta acacctaggt tgtctgagat
    34261 tttatctatg gaggatgtaa atcaggatga attctttagt tatattctaa ctattgttga
    34321 aaaggaagct ttatctccat tgacattaat atttacatat agtaattatc ctatttttgt
    34381 taaatatttt gcagagccaa gtataaaaat tgaagatagg gttacctatt tagatgagac
    34441 tttaagaaaa gctatgcatc atcaaagtga atttgctaca gacataagat ttatagtttt
    34501 atattatttt tgtatgtcag gaaaactcgt attgcaaaaa gaacagatta ataatttgct
    34561 ggaatatcct aatttagaac acactgatga aaaaatgaga atgttacgtc cgtttattcg
    34621 tgcagcagaa ttaatggttt taaatatgga aaaaaaatct gattggtatt tagatatttt
    34681 ttgggggagg attagtgtta tgactgaatg taaattatac gcaactaaat ttgaagatga
    34741 aactgctaat acagacttgt atatggaaca attacatgag gtttttgttt atttatcaga
    34801 tttactatgt aaagtaaatc cattagacga gaaaatgtta gtattacttg gaatagcaat
    34861 atattcttat aaacgattac gagaagtagt tgagcataaa ttatttaatt ccatttcagg
    34921 tagaggctca gtaagggtat tgatagaaaa ttatattatg atgaagtatt tagtaaaaat
    34981 agaagctcaa catgaaaata tatggtcaga atatcaattt tatggaatag gtcaatataa
    35041 attaattgtg gctagatata gagaacaaga acaagattta aaaaatagtc atgttgatta
    35101 taaatatatt gaagctttag ttaatgaatt tgtaattgaa gaaacaatag atatggatac
    35161 taaatatttt gataagcaaa atatacgtag taaggctgaa atagttggag aaaaagagtt
    35221 atatgggttg tactatgatt atgattccgc atatgaacat ggtttatggg gatcaataag
    35281 agaaagttca atgttaaagt gtgataatcc agctcatcac tatcattgta taccagatgt
    35341 agacaataat caaaatttaa aaagtgtttg gcatgattgt gtaaacataa tggaaaaaac
    35401 tttacttttc ttagatagtg tatatggtat accacaacat ttgctattag aggtgataaa
    35461 aaatggaaaa gaatttttta ctaaataaaa taaatagtat tcagaaggaa tatttagaat
    35521 tattagtaaa gttaaaagat aatatagata ctatagatta tatttatata atagatgaaa
    35581 taaatatatt ttggtattca tatagagatg ttataaagct ttacttaggg aatattacac
    35641 aaaaagacaa tgcatatatt tttacaggtg caacatatct agatgttgat gatttagaac
    35701 attatccatt tgttatgatg ggagatatac atatagtaga tgattcgcta tgtaaatatt
    35761 caaaaacagt actagtatta tcaaatcagg aatttgctaa agtattaaaa gaccaaatta
    35821 tttcttgtat agatgataat atcaaaatat tgtctaatta ttcttttgcg atgttaatat
    35881 taccaatgaa tttactatat ggtaatgata tgaaaataat acatgaatat tcaaataata
    35941 tatttctgaa ctttttttca agaaaagact taaacttaga tacttacttt gaagaattta
    36001 actcaatcga agatatagct ctagcattga atccaggtat agagagttat attgtttttg
    36061 acgaaggtga agatttaact aaaaatctgg aagaaagatt taaaagcttt attgataagg
    36121 ggaaggtacc cctaccagca cataaaagtg atgcttttaa attttggctg ataatttatg
    36181 gatttataac tcaggcatta gatattatat tgtcatgtaa aggatttaac ctaactcctt
    36241 atttaagata tagagttaca ttacaatatg ttttattact aggcgataat gtttttgata
    36301 ttgaagatct cagcaatatg ttgttaaaat gtaaagtagc tcatgtactt tatgcaatat
    36361 ttgataaaag cgaaataaaa aatatttcct ttgttcaatt ttataagaaa ttaagtgaat
    36421 ataaatttag ctataaagta gaagatgata tcaaagaaaa tataaatata aatcaaattg
    36481 ctgaaatttt aaataaaaat attacattgt ttttaaatta tatataaatt tatttatata
    36541 attacattcc ttagggtaca taacagaaaa aaggctgtcg cactaaaaaa tagtgctacg
    36601 ccctttttta tacaaaaaaa taaagctcaa gttcaaaaga gcctgggatt cctgtataat
    36661 aattgtatgc taaaacaaaa tactatacaa attgattata ctacttatgg taataattat
    36721 caatttaaat taccactaaa tattgattat atgattccaa aagatgctat atttattgat
    36781 ggtacaaaaa tagagtcatc tgccaataga tatacttttg tttggaaaaa aactataaac
    36841 aaaaatatgg ttagagtgat gaatgatata tctaatttca tattaaaatg tgagattgat
    36901 tttggtatta aaataattta tagaaataaa atcaaaatat atcatttaaa aaaactttta
    36961 aagaagctta aaaaaatatc aaaagaatca aatataacat ttgttcatgg aaaaggaaag
    37021 aaaaaatcta tagcgaaatt aaataattat atcgatagat tgaaaaaata tactaaagat
    37081 cttcatataa tgggtgaacg aaatagttat tctaaaacag ataatgacgc aactttcatg
    37141 agaatgaaga aagaccatat gaaaaatggg caattgaaac cagcatataa tatacaattt
    37201 ggcgtagatt cagaatacat agtttgggta agttcagagc ataaacctac tgatactaca
    37261 acatcaatac catttttaaa atctattgaa gagcatacaa aatttaaata tcctaaaata
    37321 gtagctgatt ctgggtatga aagtgaggaa aactatacat ttttagagtg taataatcaa
    37381 ttatctttta taaagcctgc aaattatgaa attagtaaaa ctagaaaata taaaaatgat
    37441 attgggcgta ttgataatat ggattatgat gaaaaaaatg attattatat ttgtaaaaat
    37501 ggcaaaaaac tatttgctaa caaaataatc aatagaaaat caaaaacagg atataaaagt
    37561 aaaataactt gttatacttg taaagaatgc tgtgattgtc aatataaaaa cggttgtatt
    37621 aagggaaata atagtaagat tcctcttgaa aatagaacta aaaatttaca agtatctaag
    37681 ttatttcatg aaaaaagaaa agaaaattta aagagaatta taagccaaga aggttgtcaa
    37741 cttagaatga atagaattat acaagcagaa ggaactttcg ctcaagtaaa acaaaatatg
    37801 aaatttagaa gatttttaag ctgaggcaaa gaaaatgtta tatcagaatg tattattgta
    37861 gcgatagctc ataacatttc tagactacac aacaaaataa taaaaaataa atgtggaata
    37921 tatcttcatc atttaaaaga agtattctaa atttattatt tatagttaga tattatctga
    37981 aaaaat
//
LOCUS       DAEMHZ010000023        37453 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000023 DAEMHZ010000000
VERSION     DAEMHZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.023"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            342..1364
                     /locus_tag="KPK72_001084"
     CDS             342..1364
                     /locus_tag="KPK72_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488320.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDMRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGFLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            1378..2265
                     /locus_tag="KPK72_001085"
     CDS             1378..2265
                     /locus_tag="KPK72_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488321.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            2271..3044
                     /locus_tag="KPK72_001086"
     CDS             2271..3044
                     /locus_tag="KPK72_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488322.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAEIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            3156..3881
                     /locus_tag="KPK72_001087"
     CDS             3156..3881
                     /locus_tag="KPK72_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488323.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            3885..5288
                     /locus_tag="KPK72_001088"
     CDS             3885..5288
                     /locus_tag="KPK72_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488324.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKHLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            5299..6123
                     /locus_tag="KPK72_001089"
     CDS             5299..6123
                     /locus_tag="KPK72_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG2488325.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6472..7092)
                     /locus_tag="KPK72_001090"
     CDS             complement(6472..7092)
                     /locus_tag="KPK72_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2488326.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            7358..7690
                     /locus_tag="KPK72_001091"
     CDS             7358..7690
                     /locus_tag="KPK72_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488327.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(7810..8451)
                     /gene="pcp"
                     /locus_tag="KPK72_001092"
     CDS             complement(7810..8451)
                     /gene="pcp"
                     /locus_tag="KPK72_001092"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG2488328.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIIISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(8472..9404)
                     /locus_tag="KPK72_001093"
     CDS             complement(8472..9404)
                     /locus_tag="KPK72_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG2488329.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANILGIILILSSLLTLSKQVVFAKYEEPDEDFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASILPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(9426..10091)
                     /locus_tag="KPK72_001094"
     CDS             complement(9426..10091)
                     /locus_tag="KPK72_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG2488330.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(10661..11137)
                     /locus_tag="KPK72_001095"
     CDS             complement(10661..11137)
                     /locus_tag="KPK72_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG2488331.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(11140..11544)
                     /locus_tag="KPK72_001096"
     CDS             complement(11140..11544)
                     /locus_tag="KPK72_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG2488332.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(11519..12148)
                     /locus_tag="KPK72_001097"
     CDS             complement(11519..12148)
                     /locus_tag="KPK72_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2488333.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      12513..12556
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12657..13625
                     /locus_tag="KPK72_001098"
     CDS             12657..13625
                     /locus_tag="KPK72_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG2488334.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGVFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            13627..14190
                     /locus_tag="KPK72_001099"
     CDS             13627..14190
                     /locus_tag="KPK72_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2488335.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            14198..14887
                     /locus_tag="KPK72_001100"
     CDS             14198..14887
                     /locus_tag="KPK72_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2488336.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15014..15742
                     /locus_tag="KPK72_001101"
     CDS             15014..15742
                     /locus_tag="KPK72_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2488337.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            15756..16430
                     /locus_tag="KPK72_001102"
     CDS             15756..16430
                     /locus_tag="KPK72_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2488338.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            16456..17292
                     /locus_tag="KPK72_001103"
     CDS             16456..17292
                     /locus_tag="KPK72_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488339.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            17308..17979
                     /locus_tag="KPK72_001104"
     CDS             17308..17979
                     /locus_tag="KPK72_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488340.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            17969..19201
                     /locus_tag="KPK72_001105"
     CDS             17969..19201
                     /locus_tag="KPK72_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488341.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            19351..19668
                     /gene="trxA"
                     /locus_tag="KPK72_001106"
     CDS             19351..19668
                     /gene="trxA"
                     /locus_tag="KPK72_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2488342.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            19685..20563
                     /locus_tag="KPK72_001107"
     CDS             19685..20563
                     /locus_tag="KPK72_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2488343.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            20833..21201
                     /locus_tag="KPK72_001108"
     CDS             20833..21201
                     /locus_tag="KPK72_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2488344.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            21204..21575
                     /locus_tag="KPK72_001109"
     CDS             21204..21575
                     /locus_tag="KPK72_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG2488345.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(21720..22175)
                     /locus_tag="KPK72_001110"
     CDS             complement(21720..22175)
                     /locus_tag="KPK72_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488346.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(22214..23479)
                     /locus_tag="KPK72_001111"
     CDS             complement(22214..23479)
                     /locus_tag="KPK72_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG2488347.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            23949..25400
                     /locus_tag="KPK72_001112"
     CDS             23949..25400
                     /locus_tag="KPK72_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2488348.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     IFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(25620..26081)
                     /locus_tag="KPK72_001113"
     CDS             complement(25620..26081)
                     /locus_tag="KPK72_001113"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG2488349.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(26186..27379)
                     /locus_tag="KPK72_001114"
     CDS             complement(26186..27379)
                     /locus_tag="KPK72_001114"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG2488350.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDIYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(27402..28067)
                     /locus_tag="KPK72_001115"
     CDS             complement(27402..28067)
                     /locus_tag="KPK72_001115"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG2488351.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(28101..29213)
                     /gene="ribD"
                     /locus_tag="KPK72_001116"
     CDS             complement(28101..29213)
                     /gene="ribD"
                     /locus_tag="KPK72_001116"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG2488352.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(29345..29466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            29655..32138
                     /gene="recQ"
                     /locus_tag="KPK72_001117"
     CDS             29655..32138
                     /gene="recQ"
                     /locus_tag="KPK72_001117"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG2488353.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      32227..32338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            32480..33781
                     /gene="thiC"
                     /locus_tag="KPK72_001118"
     CDS             32480..33781
                     /gene="thiC"
                     /locus_tag="KPK72_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG2488354.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            33835..34029
                     /gene="thiS"
                     /locus_tag="KPK72_001119"
     CDS             33835..34029
                     /gene="thiS"
                     /locus_tag="KPK72_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG2488355.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            34022..34825
                     /gene="thiF"
                     /locus_tag="KPK72_001120"
     CDS             34022..34825
                     /gene="thiF"
                     /locus_tag="KPK72_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG2488356.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLFENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            34888..35658
                     /locus_tag="KPK72_001121"
     CDS             34888..35658
                     /locus_tag="KPK72_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG2488357.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            35675..36778
                     /gene="thiH"
                     /locus_tag="KPK72_001122"
     CDS             35675..36778
                     /gene="thiH"
                     /locus_tag="KPK72_001122"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG2488358.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            36779..>37453
                     /locus_tag="KPK72_001123"
     CDS             36779..>37453
                     /locus_tag="KPK72_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2488359.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYN"
BASE COUNT        14345 a         4259 c         5981 g        12868 t
ORIGIN      
        1 taaaaataag attcaagata aataaagtta tatgcaacta tttaataatc tttccataat
       61 aaggacagta ggttctattc ttaaaaaact ccatctttaa atttaaagaa taactacaat
      121 ttgaacaaca ataaaatttc caatcaataa aaatatcaaa aataataaaa ataattgaaa
      181 atgtataatt tcccagaaaa ataataaatg taactccaat aataaacaca cctataaatt
      241 ataaaataat ttaagcttta tttaaggtta gtattatatt tatataagat ttaagtttta
      301 atatatatgt gtaattaaaa tgatttagga ggagaaatat attggtaaat tgggaaataa
      361 aaaaattggc taaatctaag agtatgttta tatctctagg gatattagtg ttaatactaa
      421 tgattagtat ctttattacg cctgttttag aaacagaaag ttattatata gataaagaaa
      481 aaggacgtat agaagatatg agaagtggta tcgacgtagg taatgagaaa tttcaaaata
      541 agattaatgt tttaaagcag ttttcaaatc aaaccgatac aggtgaattt tctaaaaaaa
      601 ttgaattaat gtcaaagaaa aaacttgata atctaagtgt gaatgaatat aaggatatta
      661 gtttttggaa agtatttaac tatagagtca caaattcact tataaatgta ggaatgttaa
      721 tcattatatc tataattata tccaatattt atgtagatga agtcgttact tcggtaaaag
      781 atatcatact atcatcaaaa gagaagaaaa aagcattaaa gtctaaaata tttgttgctt
      841 tattagttcc attagtaatt tattctgttt atttaatggg aatatttatt ataacttgta
      901 tgcaacaagg agtacctgtt aatggagact tagaatcttt tagaatagta gacaatgtag
      961 ttgcattgaa aactaattta agtataacaa agtatgtaat attaaatata ggcatagagc
     1021 ttttgatgtt tgaaggatgg gccatgatag ccatatttgg gtcttttata agtaagtctt
     1081 caatatcttc aataagtttt tttatattta ttatatctat aactaagata atgtcggtaa
     1141 tccatatatt acctaaaaaa ttactttcag ttgtatcaaa tgtaaattat tatgatttga
     1201 tatttggttt taataaaata ataggaaatt atctaggtga tattatgata tttaaagtga
     1261 atatagacat tgtcaatata gcaataggat ttttgatggc aatattgata ggtgctatgt
     1321 cattatgttt tttagtaata aaatcagatt atataagtag ataaaagggg agttaagatg
     1381 aataaattag aaatagaaaa tctttcaaag atatatggta aaaaagtagc aaacgacaag
     1441 attacagtaa ctctagaaaa tggagtttat ggtcttctag gaccaaatgg agcaggaaaa
     1501 tctacattaa tgaaacaaat aacaacgttg ataaaacctg atgaaggtca gatactttat
     1561 aatggtgagg atatatttaa aatggatgct gattatagaa atattttagg atatttgcca
     1621 caagaatttg gagtttataa aaattttaca gcaaaagaat ttttacagta tattggagct
     1681 ttaaaaggtt taaggggaaa atatttaaat agtaagatag gagaattatt agatttagta
     1741 ggtctttatg atgtgagaaa taagtctata ggaaaatttt caggaggtat gaagaggaga
     1801 gctgggatag cacaagtttt actaaacgac cctaagataa tagtccttga tgaaccaaca
     1861 gcaggtcttg acccacaaga aagagctaga tttaggaatt taatagcaaa aatgtctaga
     1921 gataagataa taatactttc tactcatata atttccgata tagaatcagt tgcaaaggaa
     1981 acaataatga ttaaagatgg caaacttcta atgaaaggaa cacacaaaga catattaagt
     2041 cacatggaaa ataaagtata taatgttcaa gttaaaaatg aatgtgaagt tgatgatata
     2101 caatctaaat ataaggttgt aagtattcaa agtgatattg attcagtagt acttagaatt
     2161 gtttcagagg atattccaag tggggataat atacaaccag ttcaagcaaa attcgaagat
     2221 gtatacatgt tttattttga tttggaagat gcaagggagg tgtagtagaa atgatgtatt
     2281 tagttaaagc tgaaatgata aaaatattaa aatcaaagag tgtttggata atttggctgt
     2341 ttttaatatt ttttggattt tttctgatta gaaagtttga tgttttagat acttatgcag
     2401 atatatttta caagatagaa ggctcaatac ctctaatagg tttgataatg tttataataa
     2461 cttctggaaa ttacattaaa gaatatgatt ctaatatgac aggtttaata aatacaacta
     2521 aaaagggaaa aaaatcagta gtattatcaa aactagtagc taatggatta attttatcta
     2581 ttattaatat ttcttttata ttgttagtag gaattagagg ttttagtttt tttgatttta
     2641 aaaatttaaa tgaaccgatt cataacttat ggtactttgg aaatagtaat ttaaatctta
     2701 cagtaataca aatgtatata attgttatat tgactactat ttttgcttct tttatatttg
     2761 cacaaatagg cttaacactt tcatctatat ttaaatcggc tgaaattcca tttatactag
     2821 gtggattgat aatggcaatt ccatacttta gtgtaggttt tataccagat aaagctataa
     2881 aatttatgtc agtgactcca aattggatta tgatgagcca acaaatggtt aagtataatg
     2941 taccaagtat tttgatagtt ttttctatag taatctcaat tatattaatg atagtgttaa
     3001 ctaaaataac atatgaaaat tttacatcaa gcaaaaggtt ttaactaaaa ataatagtaa
     3061 ttttatttat attttagttt aattaaaaat aagataaatg aattttagat aaaatatatg
     3121 ctaatatata tgtaaattat tacaggaggt tgtttatgga aaatcgtgtt ttaataatag
     3181 atgatgaagt agaaatattg aagcttttag aaactgtcct aaaaaaagaa ggccttaata
     3241 atatatatac tgcaaaaact aaaaaagaag gattagaatt atttaaaagt attaatcctg
     3301 atttgattgt attggatatt atgttaccag atggcgaagg ttatgatatt tgtaaagaaa
     3361 taagaaaaac ctccaattct ccaataatat ttttatcagc taaaaccgaa gaattagata
     3421 agttacttgg actagcgatt ggaggagatg attatgttac aaaacctttt agccctaagg
     3481 aagtggcttt tagagttaaa gcgcatttga gaagattaag ttattttagt gatgctcaaa
     3541 acgaaagtaa aaatctaaat aataatgaag aaaaaattat atcatttggc ccatatatat
     3601 taaatgaaag tagggcagaa cttataaaag atggaaaatc tataggactg tctgcaaagg
     3661 aactaaaaat cctatcttta tttgcacata atcaaaatca aattataagt aaagaaaaac
     3721 tatgggataa agtgtgggga gaagattatg ttggatttga caatactata atggttcata
     3781 taagaaaaat tagggaaaaa ttagaggata atccttcaaa gcctgaatat attctgacaa
     3841 taaaaggtct tggatataaa ttagcagtaa aggaagatta gaatatgaag tggaagatta
     3901 ctagaaattt tatatttaca attgtgtttg tagctatatc agtagtaatt ataaatatca
     3961 tatctatatt gtatgtgatt tcaactaata gtttttttaa agttgtagat tctggaaata
     4021 atcctgaaga atttgcacgt tcttttgaaa aagatttata tgaaaaagat ggagaattta
     4081 aactatctaa gattggagct gaaaaattag aaaaatcaaa ttcctggata caggttttaa
     4141 atgatttagg agaggaagtt tatggagtaa atgtgccaaa ggatactccc aaaaaatata
     4201 caccctttca aatggtgaat aattacaagt atatagaaac aaagtatgtt aattttgtac
     4261 tagaaaaaca tttaaacaaa aaacatctaa acataatagt aggtatacct agtagagata
     4321 tatcaagaat aattttaacc tattctcaaa ataacattaa aaaaacttta aataaagtta
     4381 tcattataac actagtaatt gatagcgtgg tagctttagg tgtaggatat ttatttagta
     4441 gaaaacttac aaaaccaatt tcaagcgtac tatggagtat tgagactatg gctaatggaa
     4501 attatagttt atatcttaag gacagaggta tatatgaaga agtttttaaa aatataaata
     4561 tgttagctga tactttaaga gtaaatgaag ttgagagaaa agaaaatgaa gagttgagag
     4621 aggaatggct ggcaaatata acacatgata taaagacacc tctagcatct atacaagggt
     4681 atgcagagat aattaatgac aaagattatg agtttgaaga agatgaaata cgagagtata
     4741 cagaaattat atataataag tctaagtata taaaagattt agttgatgat ttaaatttat
     4801 ctactcgatt aaaaaataat actatagttc tggacaagaa aaaaataaat ttagtcagtt
     4861 tagtcagaga tataataatt gatattttaa atgataatag atataaaaat aggaatattg
     4921 aatttgaaag taatgaagat ttaattgaag tatatgtaga ttcaatctta ttcagaaggg
     4981 ctataaccaa tcttatattt aattcaatag ttcataatag cgaaggaact ttgataagtg
     5041 tagaaattgt aaaaaaagat aatatagaaa ttataataaa agataatgga attggaataa
     5101 gcaaaagtga tttgaaacat atttttaaaa aatattatag aggtactaat acaggtgaga
     5161 tgcataaagg ttcaggtctt ggtatggcta tttctaaaga aataatagag attcataaag
     5221 gtaaaattta tgtaagtagt gaaattggaa taggaacaaa aattattata gagataaaac
     5281 aaaattagga gatatgatat ggattttttt aaaaaaggta taagtgggtt tactattaag
     5341 attttagcat taatatttat gacatttgac catatagcag catttatgcc acaaacaatg
     5401 caaataccaa tttggtttca ttgggtaggc agaatatcag caccactgtt tatattcatg
     5461 gctgttgaag gattttatca cactagtaac agaaaaaaat atataagtag attgtatatt
     5521 tggtctgtta ttatggctat aggaaatcaa ataattaata atgtattttc acatccagaa
     5581 ggagctataa ttataaataa cattttttca acattatttt taatagcgat ttatttacaa
     5641 gcaatagagt tcataaagaa atttagaaaa gagaaagaaa ttaaatattt tattatagga
     5701 ttattgatga taatcattcc tataatttta ggaatattta cagtagcttt actttttaaa
     5761 gtcactaaca gagttattac tttatttatg attttggtac cagtaccatt tttagtagaa
     5821 ggtggaccaa tttggattat acttgggatt attttttatt tgtgtagagg taaaaagttc
     5881 agtttgagta tttgttatgt tttaatgtgt atatttatat ttacaacaat gtcaaatgga
     5941 gattatagtt taaaaaattc tattttacaa aattaccaat ggatgatgat tgcttctcta
     6001 cctttaatgt tactttacaa tggagaaaaa gggaagagta tgaagtattt attttattta
     6061 tactatccaa tccatgtata tatattgtat atcttaggaa tatacttaat aaatggtttt
     6121 tgattgtaag tgatattttt aatacaaaat gaacaatatg aggattgttt taggtctatt
     6181 aaaattgtta tttagatgag atttgtagaa tatttaaatt tatgagaaga aattcagtta
     6241 ttgtaatttc aataacatta gaatataata ttataattta tttagaaata tatgatgtta
     6301 tatatggttt ggttattaat gtaataataa aacagaggtt tgatataaaa ttcaatacct
     6361 ctgttttagt gctttttatc ctacatttat gcttatttta tttataaatt agtgttttat
     6421 tttatttata aattagtgtt ttgttttaat taatattagt gcatttttta tttattttat
     6481 aaaattagtc ataattgttt cttcatattg tggttcttta attccagctt ttttacctgc
     6541 tgatatacac ttaagaagcc atgccatgtt ttctcctaga gttcgcatag tctgtaatcc
     6601 ttcttcatcc ttcataacat cctctggagt aaagccatgt acctggttcc aatactgtga
     6661 tgatacaata ggcatattgc tgattgaaaa atatttattt agttggtcaa atgttgcact
     6721 tgcaccacca cgtctacatg acacgactga agcacctaat tttgtagcca tgcgacttcc
     6781 agctatgaaa aatagacggt caagaaaaga agttaattga ccagttgcac tagaaaagta
     6841 aacaggagaa ccaataataa taccatcaat attatccaga tcttcaatca attcattaac
     6901 tttatcattt cgaatacatt ttccagtttc aaaacaggat gcgcaactaa tacatcctgg
     6961 gattgcttct ttacctaaat acagtatttc tgttttaata tcgtgtttac tcaatacttt
     7021 agtaatctca tttagtgctg tataagtaca tccatattgg tttgggctac cgtttatcaa
     7081 taaaactttc attataaatt tcctccaatg aattttattt aatagatttt ctaagagata
     7141 ttatttttta taaaatagga ttttcatgaa tattatcaat ttatatagaa atgtctaatc
     7201 ttagaatcct atttaaaata actctttttt tagtaaatac acctcctatt ataaaattga
     7261 ctttcaatta ttcttacact ataattttat tataaattat tctaaaaaca agtacgcaca
     7321 tttttgttca atactaacca aaaggagagt gtaatatatg agtatagact gtatatcaaa
     7381 tacagattta agtgaaacag gatttagtta tacactttca ctgattagtg gtaaatataa
     7441 gatgataatt ctttactgtc ttgtagaatt tgaagttgta agatataatg cattaaaacg
     7501 ttatataaat actatctcct ataaaacttt gagtttatca ttaaaagaat tagaagcaga
     7561 taacttgatt attcgtactg aatatcctca aatacctcct aaggtagaat atagtctatc
     7621 agaaagggga aaatctctga ttccaatttt agatgcaatg tgtgagtggg gagagaggaa
     7681 tagaccttaa ttttgcatct gcagatacca taaaaagact gtgagtaaaa ttgtttgaat
     7741 aatctactca cagtctatat ttaaattaat agttagtatt tttaatttat aaaaatacta
     7801 aaaaatttat tatgaaattt caccaccaat ttcttttaca tcttctttat atataataca
     7861 agcttctata gctaattcta agccttttac tatttcttct aaagacatga atggtgtatt
     7921 ttttttatct attacttgtg aagttgtata tgggatgtgt ataaatccac cccttatatt
     7981 aggatatttc ttgtctatca tataaagaat tccatacatc acatgattgc aaacaaatgt
     8041 tcctgcagtg tttgaaacac tagctggaat tttattttca ttcatatgtt ttaccatagc
     8101 ttttacaggc aattttgaaa aatatgcagg ttcaccatct tcttttataa tagtatctat
     8161 aacctggtta ccctcattat cttttattct aaaatcatct atatttatag caactctctc
     8221 aggtgtaatg tcaaatctac ctccagcttg tccaactgat attattatat ctggattgtg
     8281 ctcttgaata gctttatcta tagcttcaac agattttgtc ataactgttg gtatagttat
     8341 ctttatgatt tctgcaccgt ttatattatt attgaccctt tcaactgctt cttgagctgg
     8401 gtttattggt tctccaccaa atgggtcaaa tcctgttaac aaaattttca ttattttacc
     8461 tcctaattat attaaaatcc taaaaagtac attactaata tgtgggctat aattaatacc
     8521 aaagcaactg gagcttgata ttttataaca atataatcat ttttacattc tagcaaggct
     8581 gctggaacta tattaaaatt agcagccatt ggagtaagta gagttccaca atatcctgct
     8641 gttaatgcta atgctccaat tatagcagga tttccacctt gagacaatac aaaaggaata
     8701 ccaattcctg ccgttatgac tgaaaatgcg gcaaatgcat ttcccattat catggtaaat
     8761 ataaccatac ctaatacata agctataaca ccaaataatc tgctatcagc agggactaca
     8821 ccagatatca tggttgaaat aacttcaccg actccggctt gagtaaataa agctcccagt
     8881 gcaactaaaa gttgtggtaa tatactagca ggtcccattt gttgaagtaa tcttgagcca
     8941 tcttgaactg atgtagcagg ttttgcctta gttattataa gggctgatat taatgcaatt
     9001 attgaaccca cacctattgc aaactgagaa gaattatcaa aattagaaag gctcatagct
     9061 actactactg cagcaaatgc taatattaat gatggtataa atattttatt tttaagtttg
     9121 tctgcttgtt cttttccaaa atcttcatct ggttcttcat actttgcaaa tacaacttgt
     9181 ttagaaagtg ttaataaact agataatatt aatattattc ctaatatatt ggctggaatc
     9241 aatctaccaa atatgaatgg cagtgatata agtatccaaa ataatgatgt accatatttc
     9301 ttattgttat ttatggtagt aaaagactta tatgctataa caatcattaa aaatcccatt
     9361 agtgcgtaaa agatttccaa tgatatgttt ataaaatttt gcatttttat tcctcctaaa
     9421 taaaactatt ttttatgact tttgtttaat tttttatcat ataaggtaaa ctggattgta
     9481 gccactacaa gtgctattac tgccataata gtactagcct ttacaatatt aagtccagtt
     9541 acattgtatc caaattgagt cagagtagat gctataagta atactccact actacctgca
     9601 aataagtttt gaccataaaa atttccatag ttttctgatg cagcagataa tgcctttatg
     9661 gcttcttcat ccgattcttt tagttcatca ctattactta aaccagcagc ttgtgccata
     9721 ggattaacaa gtggtcttac aaattgagga tgtccactca ttcttattga tagagcacca
     9781 gctatttgtc tagctatcat gtatatagta agtacttttc ctgttgtaag tctttttaat
     9841 ttttctatta aagaaacagc tctttgtttt aaaccatatc tttctaatac accgattact
     9901 ggcaatgtta atatgaataa tgatacacct ctgtttgaga caaaagaatc tcctaaaatt
     9961 gtaaaaattt gattaaaatc taatcctgca acaattcctg ttgccactcc tgctaataat
    10021 acagtaaata gtgtgtcaat tttaagtata aatccaatta caactatcaa tataccgatt
    10081 agttttatca taaattgttc tccttccatt tctatatgtt ttaatttaaa tttatttaat
    10141 ggataaagtt aaactctttt tatagtattt gcgttaatag gactattatt ataaaattta
    10201 ataaaaaata gggaagatgt tttaaagtat caaagtaaat aatggttgtg tataggaatt
    10261 tgaataacaa agattatatt ttaagctaat atgaattgat tttgtaagtc tatagtttaa
    10321 gtatatgtca atatttgaaa taggttttaa attagatatt atattataaa aagagaatgt
    10381 gtcaaacata gagacaagtg tatttttcac ttttgaaatg ataaaaatat atttgatttc
    10441 atacaaaaag agagtgtcta atgaattttt tagttcatta gacactctct ttttgttatg
    10501 attaataaaa catatttgtt atgcttaata aagcatatat taagtgctag tttcattaga
    10561 tttcactaag aaaaataaaa ttttgataaa gtttaaagac tattttgagc aatattactt
    10621 ataaataaaa gaatttatca tagaaataat tagttttatt ttagctatta agatttatta
    10681 aaaaattatc tatattatcc tgtgatattc taaaactgtc ataattacct tttgaaacag
    10741 ccttcataat aagtttatct aaaaatttca ttttttctaa tcgagcttca ctaccaaaaa
    10801 ttccaatagc atttgcactt tctaataagg atgatggaaa atttgatttt aaatatttat
    10861 ctgcttgttc agaaaagcca cagcaaagga aaatgccaac ttgttttttg tttaataact
    10921 ctacattatc attacatagc acttgtattt tttttgaaac tgaaccaaca tatatagaac
    10981 ttccaataat aattttatca aattttgata actcaatttt attattatta ttattaatat
    11041 caacaatagt gacattatct gatagtttat tttttagtat atttgcacag tcttttgtac
    11101 aaccatattt acttgaatat ataataatag tattcataat tattctccct ttcttaaagc
    11161 attttctaca gcttttcgta tagtattact gctataagtt gcagaagtta tagcatccac
    11221 atctaaagac tgttgtttaa taatacttgt tgtaattttt tcagcaggtt tacctaaaag
    11281 atggtcgtgt tttaagatat taatattttg gattttacca ttattaactt caacagacac
    11341 agtagctttt accaagttat tatcagcact tccagtataa atgccatttt taacattatt
    11401 aatatctata tcttttacta ccaagttttc tggctttaag ttgcttacaa ttacaataat
    11461 aatgcatata aatacaaaga atagagcaat agatattttt acatattttt tctttttatt
    11521 agtagccttg ttgcgaattt tcattgtaaa gtcctcccac taaaatatct aagaaatttt
    11581 ctataagttg agaaaaatta tgtaaagaat atagtttatt ttcaacaaca cataaaacga
    11641 atcctaacaa tgcacttata agcatccatg agcttttatc tatatttttt ttgaatattc
    11701 cttgttcaat tccaatggaa aggaatttat caagcatttt tataccatta ttgctaggcg
    11761 tattgtttgt attttcagat gcaaatataa tattcattct actatgcatg atagattttg
    11821 ccatctctgg ctcactagaa agagttttta taaagctagt cataatttct atgatttgtt
    11881 tatctattgg tagtgatgag caattattca taatgtcaat ggcattactt tctactgtgt
    11941 tataaagttt attggtcata tcgctgataa tctctgcttt gtctttgtag taaagataaa
    12001 ttgtagttgg agaatattct atttttgtag caatttttct tattgataaa ttttcatatc
    12061 catgttggtt tataatttcg atgctagcat ccataatctt tttcttcatt tcttcttttt
    12121 catttttacg tctttctttg atactcatat aatcacctct ttgcttaacg gtgttaagtt
    12181 aatttacact gttattgtat aatttaattt taatactgtc aataaattat taaataatat
    12241 aagttaatat atttttatgg ataataaatt aatgttatta attgtaatat ttaagatatt
    12301 attgtataat aataatcggt ttaaatgcgc cacatcttat taaagttggt tcataattct
    12361 aaataaactt taataagata cattaaaata taataaattc ttttatgcta taaaatttat
    12421 tttatagcta ttaaatcaga tgtttaaaaa ttttttaaca gtctatagtt tttaagagtc
    12481 tatagtaagt agactaaaaa tatgatttta catctgtact tgttcgagaa ctccaagtat
    12541 aaaaaactaa ggaattatat agtggcagta tataattaat acccttagac acaccttttt
    12601 gaaagtggaa aaaagatgtg tagttctcct cgaccacaaa tcaaggagga taaaaaatgg
    12661 aaaagagaaa agtaataatt gattgtgacc caggaattga tgattctttg gcaattcttc
    12721 tggctttaaa ctcaccagag ctagaagtaa ttggaattac cacatgttgt ggaaatgtcc
    12781 cagcaaatat aggtgcagaa aatgcactaa aaacacttca aatgtgttct tcactaaata
    12841 ttccagtata tataggagaa gaagcaccac taaaaagaaa acttgtaaca gctcaagata
    12901 cacatggaga agatggtatt ggagaaaact tttatcaaaa ggttgtagga gctaaagcaa
    12961 aaaatggagc agtggatttt ataataaata ctttatataa tcatgaaaaa gtatcaataa
    13021 tagcacttgc accacttaca aatatagcta aagcacttat taaagataag aaagcatttg
    13081 aaaatctcga tgagtttgta tctatgggag gagtatttag gattcatgga aattgctctc
    13141 cagtagcaga gtttaattat tgggtagacc cacatggagc agattatgtt tacaagaatt
    13201 tatctaaaaa aattcacatg gtaggtttag atgtaactag aaaaattgta cttactccta
    13261 atattattga gtttataaat agacttgata agaagatggc aaagtatata actgaaataa
    13321 ctagatttta tattgatttc cattgggaac aggagggaat aattggctgt gtgataaatg
    13381 accctctagc agtagcatac tttatagata gaagtatatg taaaggattt gaatcatatg
    13441 tagaagttgt agaagatgga atagctatgg gtcagtctat agtggattct ttcaatttct
    13501 ataaaaaaaa tcctaatgca attgttctaa atgaagttga tgagaagaaa tttatgtaca
    13561 tgtttttaaa gaggcttttt aaaggttatg aagacattat agactctgtg gaaggagtga
    13621 tatagtgtga aaagaaaaat atgtgtaaaa actattacac ttatagcttt tggtatttct
    13681 ataaatataa taggcgcttt tatagctatg ggattaagat tacctgtata cttagattca
    13741 ataggaacta taatgatagc ttcactttta ggacctaaat acgctgttgt tacaggtgtg
    13801 tttggaagtt taataagtgg aattacgttt gatgtgtatt ctctctattt tgcaccagtt
    13861 caaatatcaa ccggattact tgctggaata gcatttaaaa agaatttttt aaggggttta
    13921 aaaactccac taggagtact tttatttgcc attccaacat ctataataag ttcaataatt
    13981 tcagcatttt tatttggagg tatgacctct tcaggttcat catatatagt gcaaatcttg
    14041 aaagtgttag gactgggaga tgtttttagt gtgtttgtaa cccaagtatt tactgattat
    14101 ggagataagc tgttagcagt tgttttagtg aatttaggat taaatgcagt tccaaaaact
    14161 ttaaaggtat cattgactgg aggaaaatag atagaaaatg gatagatata gtgagataac
    14221 aaataaaaat caaagagaaa ttgttctgct taaaggattt ccttgtatat ggggaaagtg
    14281 ttctttttgt gattatatag atgataattc taatttagaa gaagagatga ataaattaaa
    14341 tctaaaggtt ttaaaaaacg taactgggaa atatggagtt cttgaagtca taaattctgg
    14401 aagctgtttt gaattaccta aggatacttt agaaaaaata aagtgtatta taaaagagaa
    14461 gaatataaaa aaattatttt tagaaagtca ttggtcttac aaaaataggc ttaaggaaat
    14521 gagagagtat tttgaaattc ctgtggtatt taaaattgga gttgaaacct ttgacaatga
    14581 ttttagaaat aatattttaa ataaaaatgc aaattttaag actccacaag atgtaaaaga
    14641 atattttgat tcaccttgta ttatggttgg aataaaagga caaacaaaag agatgataga
    14701 taaagatata gagattatcc taaatacttt tgaaaaagcg acagtgaatg tttttataaa
    14761 taattcttct agtataaaaa gagatgaaga gttagtgaaa tggtttgtta gtaagtataa
    14821 attcttggat gaaaatccaa atatagaagt tttatacaat aatacagact ttggtgtagg
    14881 agattagcat taatttagtt taaatttaaa attgaaaagt ttcttatgtt tttgaaaaag
    14941 cttctaatag ttagaagctt tttttgtaga gttttatgtt acaataagaa aaaaggaatt
    15001 aaggagcaat taaatgatgt ttgatagggg aaaatggaat aaattggatt ataatgaact
    15061 tttagaatat atgatgtcta tacaagatat aaaatataga gattttaata aaaagcttat
    15121 acctggaaca gaaaatataa taggtattag agttcctaat ttaagaaagt tatcaaaaga
    15181 aataagccaa gggaattgga aggaattttt agaagtagca gaggatacat actatgaaga
    15241 agttaggcta caaggtatgg taattggcaa tataaatagt aattttgaag aaactttata
    15301 ctatgtaaaa agatttatac ctaaaatcga taattggtca gtttgcgatg gattttgttc
    15361 tgatttaaag tctatgaaaa aatataaaga aaatatgtat gatatactta aaaaatatgt
    15421 ttattcaaag aatccatggg aaattagatt tgcattagtt atgtttttaa tatattatgt
    15481 tgatgataaa catattaaag aaatatttga atactgtaat aatatacaaa gtgaagaata
    15541 ttatgttaaa atgggtatgg catggttgct atcaatttgt tttattaaat gtgaagagga
    15601 aacttttctc tatataaaaa ataataattt agatgatttc acttataata aaatgctaca
    15661 aaaaataatt gaatcaaata gagtagattt agaaaagaag aatataataa gaagtatgaa
    15721 aagaaagaca agaagatgtt gacggaggaa tattaatgaa taacaatgaa gacagaattg
    15781 acaaattttt agaacttcaa agcaaaggat ttaaatttgc agagatagct caaaatctaa
    15841 agattagtca aagtacttta agaacttttt taaataaaaa aggatataag tcagaaaaag
    15901 gtaaatacat attaaaagac gaattaaatt ataataaaag tttggataat agaaacttgg
    15961 aagaagagaa agctaaggag tatgaaaaaa gtaaaaagaa tactataagt aatagaaaaa
    16021 aagtaagcac caaaaaaaag atttctaatg aaaaggcttt aagtaaaagt gttgaacaaa
    16081 ttgagtttaa tgatacaggt ttaaaaaatg caaagccaaa acaaaatgtt aagcctaaga
    16141 aagatagaaa aataaatata actcaagaag atttagataa gctttgtgaa gtatatgatt
    16201 ggtatttaga agtaaaagat tacaatataa agcctaaaaa agtatctaat aaaaaagatg
    16261 tacatataga tgatgaacac atagatgaat taaaaacaac aagaatacgt atagataaag
    16321 aaatatggaa tgattttgaa agattatgta gtaattcgaa ttttgaaaaa catcaaatac
    16381 ttacacaggc attgaaagaa tttatgaaaa agtataaaaa tctactataa aatactacta
    16441 tatgaggtgc ttaatatgaa taaaatagta aaaaaatcaa tttctctaat gattatatta
    16501 acaatattta tttttatgtt gacagcatgt gaaaaagatg aacagcctga ttcagttgat
    16561 attcaaacag aagataaaaa tgagataaaa atagatgagg gaaatgcaaa agtattgaat
    16621 ataggaaaat cagaaattat aaatatagat gaaggtgata aagtagatac atcaacaaag
    16681 gtagaaaata attcaacatt taatatatcc aatgttgaat tgatatataa tgaatatgat
    16741 gagaataaaa aaattatatc ttcggatagt aaggctttgt tggacatgac tttaatgcct
    16801 gggaaagttg cttacataga atgtgggcat aaaacttttg taaagggtgt agaagtttat
    16861 gcatatgaat atgaggcaga gggtaagatt gtgtatgtta atttaaaaga aaatactatt
    16921 aatataagaa ataataatat taagctagaa aatagtagtc agtatgaggt gctatctaca
    16981 tcagaactta aaaaagtaaa tgaatcaaat gagggaatta cgtatcaagt aaaagtaaaa
    17041 aattcatcat caaaagattt aggaaatatt attcttaaga cagcagaagt taatgaaaat
    17101 ggagaatatt taacagtaaa tcgtgtgcca tcatataaga ttttaaagcc ttcagaagag
    17161 acagatatag atatattgtg ttcaacaaag gcaaaaagtg ttgaaatagt gggatatacg
    17221 tatgatgata taaaagaaaa agccaatgtg gatattgact taaaatctca taaagttaag
    17281 atagacaaat aaaggtaggg gatacttatg aaggttttaa tagttgaaga taataagata
    17341 cttttagaaa gtgtagtaga agaacttagc aagcattttg aaactgaaaa gtgtgaagat
    17401 ggagaagaag ctttatactt agtaaatcaa aatatatatg atttagtcat tttagactta
    17461 atgcttccta atataaatgg atttgatatc ctaaagaaaa tgagagttaa taatatagat
    17521 acacctgtac ttatattgac agcaaaagaa acattagatg acaaagtaga agcatttact
    17581 ataggagcta atgattattt gacaaaacct ttttatatgg aagagttagt agctagggta
    17641 tacgctatat taaggactaa tggaaaaata aaagaaagaa acggattaga atttaaatct
    17701 ttatatttag atacactaga aaaaagagtg tatattgaaa aagaagaaat aaaacttcaa
    17761 aacaaacagt ttaacttgct tgagtatttt gtattaaata aaggttcaat tttattaaaa
    17821 gagcagattt atgatagaat atggggaatt gattcagatg cgactattga aatagtggaa
    17881 gtatatgtaa gtaatctaag aaaaaagcta agtaaatatg gatatgataa atatattaaa
    17941 actaaacgta aggtaggata tatattcgat gataaataag aatgtattta ccagtactaa
    18001 aaatcattta ataaaaatgt atattattgt agtaggtagt tttttaataa tcttttctat
    18061 atttatatac tcatatttta ggggacttac atatagtgga attgatagtg agataaatga
    18121 tgaacttgaa tatatagtct ctcaatttaa aagaacttca tttttgaacc caataagact
    18181 gaaagatcct aaagatatgg tgtatgtata tgaagatgga aggataagtt actatactca
    18241 aaatgaatat tttgatgagt tacttccaga tagaagacta gataaaaaaa attcgttttt
    18301 taaatacaca gaaaatggat atacttttag agaattaaat gtagatgtag gtagatacca
    18361 aatacagata attagaaata tagattctga gatgaattct ttaagacagc ttacatctgt
    18421 tcttataatt ggaatattaa tttctgtaat aattacttac tttgtggctg tatatctcac
    18481 tagaaaagct ttaattccaa tagaaacagc atggaaaaat caagctaagt ttatacaaga
    18541 tgcttctcat gaattaagaa caccaataac aatagtttca tcaaaattag aaagtatgtt
    18601 aaaatcccca gaaagtacag taaatgatga agttgaaaca atagctactg caatgaagga
    18661 aactagaaga cttaaaaaaa tgataactga tttattgtct cttactaaag aggactctat
    18721 tgttaaagtt aatttagaag aaatagattt agaaaagtta ttggaagaaa tatcagaaga
    18781 ctatattgac attgctgagt ttcaggaaaa aagattcgtg tttaattcta aactgaagaa
    18841 taaagttata ataacagata aaaataaatt aagacagctt atattaattt tcattgataa
    18901 tgcctttaag tatacaaaat tgggtgatga gatagcatta gaattgaaag aagatataga
    18961 ggatgaggta actttactta taagtgacac tggtataggc ataaaaaaag aagagatacc
    19021 tcttatattt gatagatttt ttagaagtga aaatgtaaga aataaagatt tagaaggttc
    19081 tggaatagga ctttcaatag ctcgcatgat tagtttaaac ttatctattg atataaatgt
    19141 aacttctgat gtagatattg gaactacatt tgaattgtca ataccaaaaa agttaaaata
    19201 ataaaaatat aaaaaattgt ttttagaaat gaagaatggg tatactaatt agtataaaat
    19261 ataaaaagac cttataaaag taaatttata tttataaatt tcatagtata attatatagg
    19321 gataataaag tttaatagga ggatttaaaa atggcaaaag ttataaatac atctgaattt
    19381 agaagtagtg ttgaaggtag taagggagta gttgtagttg atttctttgc aacttggtgt
    19441 ggaccttgta acatgttggg acctgtattt gcagaattag gtgaagagat gaaggataaa
    19501 gctagatttg taaaagttga tatagatgag agcttagaaa tagctcaaca attcaatgta
    19561 tctacagtac caactatgat tatatttaaa gatggaaaac cagtagaaac tttaattgga
    19621 ttcatgccaa agaacaaaat tgaaatgcaa gtaaaatcat atttataaaa aatgggtgag
    19681 tattatgaga tatgatatag ctataatcgg cagtggtcca gctggattgt ctgctgctat
    19741 aaatgcaaaa ataagaaata aaacaattat catgtttggt aatgataatt taagtaataa
    19801 gcttgttaaa gctccttcaa tagataatta tttaggtttt tatgatataa gtggtgatga
    19861 attaaaagac aagtttaaat cacatataga tagtatggat atttcaattg aaaataaaag
    19921 aataaataat atttatgcta tgggagaata ctttacaata atgtctggaa atgatatgta
    19981 tgaagctact actgtaatcc ttgcaactgg ggtagagtat actagaccaa tcaagggtga
    20041 agaagaattt ctaggaagag gtgtgggcta ctgtgctaca tgtgatgcac cactttatag
    20101 aaataaaaag gtagcagtta taggatataa tgaagaatct aaagaagaag caaatttttt
    20161 aagtgaactc acttcaaaga cttattttat acctatgtac aaaaaagata atcttatgag
    20221 aagttcagat aacctagatg attctattga agttatacat gacagaccag tacagataga
    20281 tggagataaa cttgtaaaca aagtatcttt taaagaaaac cacattgaag ttgatggagt
    20341 attcgttata aaagatagta cagctccaag tgcattggtt cctggaatag agattgatgg
    20401 cattcatatt aaagttgaca ataatatgaa gactagtata gatggatgct ttgctgctgg
    20461 agattgtgtt ggaaaaccct attcttatat aaaagcagct ggtcaagggc aaatagcagc
    20521 tttaaatgct gtttactatt tagataaatt aaagagggct taatatttat attgattaac
    20581 tttttaaaga tatttataaa aaactgtctt gaaataattt tattatttca agacagtttt
    20641 ttatttttaa gttattaata aaagtatttt aaaatctata tgtaattact tttattaata
    20701 aaactcaaag ttatattagt aaaaattgga attatttata atatactcca aaatttaaaa
    20761 ataatggttg aaaaatctaa ttaagagtac tataatttaa ttaagtaatt acttaatcaa
    20821 aacgaggtac ttatggaaaa cttggttaaa atatttaaag cattgagtga tgaaactaga
    20881 cttaacatac ttatattagt atccaagaga aatatatgtc aaaaggggat ttctaaatat
    20941 ctagggattt cagattctgc tgtttcacaa catataaaaa tattaaaaga tgttgggata
    21001 ataacaggat ataaagaagg atactatgtt ttatatcata ttaataaaga atcatttaat
    21061 atatgtgtag attttataaa ttctatgtta agtaattcta gtgaaagctt tatagatgta
    21121 tttgatgtaa atacaataca tctaggatgc agtaaagaat gtaaatctat aaaaaaatgt
    21181 tgtaagagaa gggagaaata ataatgaaag tatgtatacc agtagaggag aataaaggat
    21241 tggatagtaa accatatgga cattttggtt ctgctccaat ttttgttgtg tgtgatttag
    21301 aaagtggaga agttaaatct ttagataatg gagatttaga tcatgaacat ggaaaatgtc
    21361 aaccattaaa agcattgtca gggacagctg tagatgcagt agttgttgga ggaataggac
    21421 aaggagctat aattaaatta aatggaatgg gaataaaagt ttacagagca gaaggtgata
    21481 ctataagtgc taatttagac ttacttaaaa atggaaaatt agtagaattt ccatcagacc
    21541 atacttgctc acatgacgga tgtggacatc attaaaaaaa aacagctgat gaaatatata
    21601 cttcatcagc tattttttct tttaataaat acgattttat tcttttaaaa ccaaaattta
    21661 gatttttata ttttttgtag tatccttaat aatatttcaa gtttacaagt ttactacttt
    21721 tagatttttt tggtatgttt ttctatatag tcaatatcat ttaatatgtc attgagttct
    21781 ttattaagtg tttttaaatc atcaccatct acattgtagt ttaaaaatag ttcctctaca
    21841 ttatcaatga tagaattgac ttttctagta acttcaacaa atgtcttaaa gttactacta
    21901 tttttagcat ttagaaattt ttttaaatcc tttgatataa atgatactat ttcatattca
    21961 tcattaaata gacctctacc aataggatat ggttcaattc tctgtaaaaa atattggtct
    22021 ttagcattaa tattataagt ataagatatt tcccaaccgt ctcctaaatc aaacttgata
    22081 aaatataatg tactacttac ttttgatatt cttgtggcac caagctgtct taaaagaact
    22141 tcaatcgaat caccttgaac agtattaata ttcatatata cacatcccct ttagtattat
    22201 tatactatat tttttaaaat tttataatta aataaacagt agatataata atagatataa
    22261 tcataagtgg aaaacctatt ttgaaatact ccttaaaaga gataggatga ccgtgtttat
    22321 tacctatacc tgctagtaca acattagcag atgctcctat tagtgttcca tttcctccaa
    22381 gacaagctcc aagagatgtt gcccaccata gtggcattac atcaatacct tcagcttgca
    22441 tagtcaaaat aagaggaatt agtgttgcaa caaatggaat attatctaag aaagaagaga
    22501 ctatagctga caaccataat atcaacaaca ttgtaaatac aagatgtcct tctgtaagtc
    22561 caatcaatgc ttgagccaat ttgtttatta tcccaacttc tactaatcca ccaactacta
    22621 caaataatcc cataaagaat aaaattgtag accattcaat acctgccata atttcatcta
    22681 catcttgttt tcctattaaa agcattatag atgctcctgt cagtgctaca acagaagaat
    22741 ctatgtggat tgtactgtga aacataaaac caaaaagtat gaatgctatt aatataaggc
    22801 tttttataag tagaggttta tctttaacag attttttttc atctagtttt aatattgcat
    22861 ttttagctct ttcattcaca acaagttctt ttccataaat aaatctaaag catattatag
    22921 ttatagcaag tattactata acagcaggac ctaaatttat tacaaaatcc ataaaactaa
    22981 gattggcagc actaccaatc ataatatttg gagggtctcc tataagagta gctgttcctc
    23041 ctatatttga agctaaaatc tgtgttatta aaaatggaac tggattaagt cctaatattt
    23101 gtgtaataac tatagtcatt ggtccaataa gtaagacggt tgtaacatta tctaatacag
    23161 ctgataaaat agctgttata atagcaaagc aaatcattat cttccatggg tcacccttag
    23221 ctttctttgc agtccagata gctatatatt caaataaccc cgagtttttg acaacagcta
    23281 caaatagcat cataccaact aatacaccaa tagtattgaa atctatatgg cttaatcctt
    23341 catcaagtgt aagtatattg aatattatca ttaatagtgc accaccaaca gctgctacgg
    23401 atctgtttac cttttctgta ataatagctg ccataactat taaaaatata catatagcta
    23461 ttatttgttg aaattccata atataagtac ctccatttgt attattttgt gcctaatgct
    23521 agtactatat atagtattac ttttttattg ttttttcaat ggatgtatga aagtatatta
    23581 tttaacaaag tttttgaaaa attgacagaa tttaaagaaa atttttgtta tttaaataaa
    23641 ttttaaaatt attccattga taataatatt aaacgacaaa cgaaaaaata taccctttaa
    23701 actagaattt attatatcta ataacaatta taaaatatta aatatatcta tattataaaa
    23761 acaataaaaa aaggtttgat ttgaatataa aactgcaata tattaaaaaa attattcatt
    23821 ttaaaccttg taatttgatt ttataaatat tataattaaa ttaatgaatt tttaacaagg
    23881 aattattcat ttttttgatt ttaagattta ttgagaaaac aaaactaaat aggggggtaa
    23941 ttaatataat ggattttttc aatggtattt taacgagtat taacaatgca gtagtatatg
    24001 taaacaatta cttatggtcg tatatcttaa ttataatgtt agttgtaatt ggtatttact
    24061 ttactattaa aacaaatttt gtacaattca gatattttat agaaatgttt agacttctgg
    24121 gtgatggtac agcgaataaa aatgcaaaaa aggaaggaaa aatctcttca tttcaagcat
    24181 tttgtataag tacatcttca agagttggaa ctggaaatat agctggaata gcaattgcag
    24241 tggtagcagg aggacctggt gctgtatttt ggatgtggct tatagcatta attggttctg
    24301 catcaagctt tgttgagagt acattagctc agatttacaa agtgaaagat ggtcaagctt
    24361 ttagaggtgg accagcgtat tatatggaac aaggtctaaa taaaaaatgg atgggtattt
    24421 tattctcaat acttataact atatgttatg gttttgtatt taatgcagtt caagcaaata
    24481 ctgtttcact tgcatttaat aatgcatttg gaataagtaa aatggctatg ggaataggtc
    24541 ttggaatttt aactgcaata gttatttttg gtggagtgca tagagttgca aaagtttctg
    24601 aaataatagt tcctgtattt gcaggattat atatattagt tgctttggct atagttgtaa
    24661 tgaatataac agagatacca agtgttatag cattgatagt tgaaagtgca tttgatttta
    24721 aaggaatggc gattggtaca tttatgggag ttgtaatgac tggagtgaag agaggtctat
    24781 tttctaatga agcaggtatg ggtagtgctc ctaatgctgc tgcaacagca catgtaactc
    24841 atcctgtaaa acaaggttta attcaatctt taggtgtttt tacagatact ataataatat
    24901 gtagttgtac agcattcatg gtattattat attcaggtta tagtagtagt ggagcaactg
    24961 gaattgaatt agctcaagaa gctttaactc atcatatagg accagttgga aatatattta
    25021 tagcagtttg tatattcttg tttgcattta gttctatagt tggtaattac tattatggtg
    25081 aatcaaatat ggagtttatg agtggaagta agactaaact taatatattt agagtctttg
    25141 ttgtaggtat ggttttattt ggttcactta ctcaagtaga tgtagtttgg aatcttgcag
    25201 acttatttat ggcattgatg gcgataataa acttaatagc aatagcactt ttaggaaaat
    25261 atgcatttat tgctttaaaa gattatacaa gtcaaaaaaa atcaggaata aaagaccctg
    25321 tgtttattgc agatgaaata gaaggcctag aaaaggtatc agagtggcat agggaattat
    25381 ctgaggaaca tttaggataa aaaatagtat aaaattataa aaccattttg aaagactaga
    25441 gtatgtatat actttatatg aatataatat tgaattaatt tatattactg gaatttttaa
    25501 ttccagtaat atttttttat tatataattt agctttatta aaagttttta ttattaaaaa
    25561 tagcttattt atcgtaaagt gaatgatatt gggtttttaa ttattatgaa tgttagtatt
    25621 taatctagag acttaaataa atttgccatt tctagcgcat tcatagcaca atcataacct
    25681 ttatttccag cttttgtacc tgctctttca atagcttgtt caatattttc tgtagttacg
    25741 attccaaata taacaggtaa ttcactgtct aaagatactt tggctatccc tttagagact
    25801 tcactacata cataatcata atgagaagta gcccctctta taacacagcc taaacagatt
    25861 atagcatcat acttaccact tttagccatt ttttgagcaa caagaggaat ctcaaaagct
    25921 ccaggtaccc aaactatttc tatattttct ggagaaacgt catgtcttaa aagacagtct
    25981 tcagctcctg ataaaagttt tgatgttata aattcattaa aacgagaatt tattatacct
    26041 atttttaaat cttttccaat aagttttcct tcatatatca tttttatttc ctccaatttt
    26101 atttatgtta tagatttata tttataaata tctttaattt aattataaaa aactacttag
    26161 tagaaaaata tcatcatata atcatttata taatatctaa aagatgtccc attttatctt
    26221 ttttagtttt taaatatagc ttattaaagt cattaatttc ttcattaata gggattctat
    26281 tttcgatttt tattccatat ttctctaatt gatttatttt atctgggtta ttacttaaaa
    26341 gatttatact ttttatgttt aaatttttta atatttgagc agccatatag aaatctctca
    26401 tatcttcttc aaatccaagc attaaatttg cttcaacagt atcgtaacct tcttcttgaa
    26461 gtttataagc cttaatttta tttaaaagac caatacctct accttcttgc ttcatataaa
    26521 ttaaaactcc acttccattt tcaacaattt tcttcataga agattctagc tgtaatccac
    26581 aatcacattt tagagagtgg aacacatctc ctgttaaaca ttcagaatga agtctaacta
    26641 gagtattttc tatatttata tttccataag taagggcaat gtgttcttca tttgaataaa
    26701 tatctctata tcctatgatt tcaaaatttc catattttgt cggaaggaaa gcactagata
    26761 ctttttctat agtagtttca tttattttgc ggtattctat aaggtctttt atagtgatga
    26821 tttttaaatt atgcttttct ttgaaaatca ttaaatcatc aactcttgcc attgtaccat
    26881 catctttcat tatttcacag ataagaccaa tctctttaaa acctgcaagt ctagcaaggt
    26941 ctactgaggc ttctgtatgt ccatccctga caagaacccc accagttttt gctattaaag
    27001 gaaagatgtg accaggcgtt ttgaaatctt tacttgtaga attttcatca cagaacaatt
    27061 ttacagtttc agctctttca tatgcagata taccagttag agtatctgct ccatctatag
    27121 taacagtaaa ggctgtttga aatgtatctg tattttctct aaccataaga ggtatattaa
    27181 gacttttaaa ttttttttca gtggcaggta gacaaattag acctctacca tatgtagcca
    27241 taaaatttat tgattcagga gtagccattt cagctgccat caataaatct ccttcattct
    27301 ctctactttc atcatcaact acgattacca ttttcccatt ttttatatct tcaatagctt
    27361 cttcaatagt attaaacatg taattcactc ctttattatt tttaaaaacc atttttaaat
    27421 aaaaaatctt tatctatatt tgatttatgg ttactactat tagaatttaa ttccttgtag
    27481 ttattagcca taaaattatt aacatacttt ccaattacat cgctttctaa attaacgaaa
    27541 tctccaacat tctttgtcaa gagaatagtg ttaattttag tatgaggtat aatagaaacc
    27601 ttaaatattt catcatctaa atatgatata gttaaactta caccatcaat agcaacagaa
    27661 cctttgagta tcattgagga taaaagtttt ttatcaggtc gtatacttac taaaactgca
    27721 ttttcatctt tttcaaattt gcagatagtt cctttaccat caacatgacc tgttactata
    27781 tgaccaccaa atcttgaaga aagactcata gctctttcaa gatttacctt gtcatttgca
    27841 tttaataaac ctaaatttgt ggaacgaatt gtctccatca taacatcggc tgtaaattca
    27901 ttttttccga ggtttgaaac agttagacaa actccattta cggctatact atctccaaga
    27961 tttgtgccat ctaatatttt atttgatagt atggttactt ttccactctt accattagca
    28021 gtaatttttc taagtattcc aacttcttct actatgccag taaacatcct aacctcctat
    28081 atgaatgtat taacaaaaat ttaatttaaa acatctgatt ctataagaat atcgccatct
    28141 atcaaagtta cattattgat tgttaactgt gttgaatctg acaaatgatt tattccttct
    28201 ccagaaacga atgtaggtgc atctttacca cctataatct tgggcgatag aaataatttg
    28261 actttatcaa ctaacttatt tctaaagaga ctgtcattga gagtagcacc accttcaact
    28321 agaacagaat caatgtttaa ctctcctaat ttatacatta caaactccaa atctactaag
    28381 ttgttttttg aaggagaaat tattgtttca acattatttt cttttaacat agataatttt
    28441 atactttctt tgcaacatgt gaaaactata gttttactgg ttttatcttt aaccaaatta
    28501 ctattaagtg gcaactttag gtgtgtatct attactattc ttgtaggatg agacactttt
    28561 tcttgattga gcctacaagt tagtaaagga ttatcgttca aaactgtatt tatgccaacc
    28621 attatacttt ggtatttatt tctgtattta tgagttagat accttgatga ttcattagat
    28681 atccattttg attctagtga ttttgtggca attttaccat ctagacttac tgctgatttt
    28741 acaatacaaa gaggtctttt gttttttata tgataaaaaa atgcctcatt taaatctctt
    28801 gcttcatctt ctaatattcc cacagtaaca tcaatattgt tatcttttaa tttttttact
    28861 ccattaccac aaacaagtgg atttgggtct aatgtagata ttacaactct cttaatctta
    28921 ttttgtataa ttttatctac acaaggaggc gttttcccat aatgagaaca tggttctaag
    28981 tttacataaa gagtagagtc ttttagactt tgtttagcac tgtttatagc atttacttca
    29041 gcatgattag agccaaattt ctcatgatat cctttgccta ttatattact atctttaaca
    29101 attacacatc caactaatgg atttggattt acaaagcctt ctccattttt agccaattca
    29161 atagcttttt tcatataata aatatctttt tctttttgat tcacaaaatt cacaatatgt
    29221 acacctcctg tttaattgga taatttttaa acaaaaaacc ctgagaatta actcagggtt
    29281 tagaataatc tacaataaat gtttaataaa tgtagaacaa aattgctcta caaaaacact
    29341 cattaaatat cttcttccat ccagacttta ctgtcggctt tggaatttaa ccaaatcaac
    29401 caagttttac attggctcgc gggctatacc gccgattggg aatttcaccc taccccgaag
    29461 attaagtatt aaatttatat ttagaatgta tcataaatta ttttactttg caaactattt
    29521 ttttaaaaaa tatatgttaa tatataatat agaatgtcta taaactttga aataccacga
    29581 ttgtgaatat aaaaataaaa atttgaacat aatcaggaaa gaagtctaat taatttaaat
    29641 taggagagta atatatgaat aaaaaacttt tagaaatact atcaaaatac tatggatata
    29701 caagttttag aaaaggtcag gaatctataa taaattctat cttaagcgaa aaagatgtgc
    29761 tagctattat gccaacagga gggggaaaat caatatgtta tcaaattcca gctttgattt
    29821 tagatggaat gacaatagta atttctccac ttatatcact tatgaaagac caagtagatg
    29881 ccttaaagac tatgggaatt agtgctgctt ttataaatag ttcattaagc agcaaagaat
    29941 ttaatgaaat attaaataat attagaaaaa ataattataa aatactctat attgctccag
    30001 agagattaga tgcacaagag tttattgagc taataaataa taacaatgta agtcaagttg
    30061 cgatagatga agcacattgt gtatctcaat gggggcatga ttttaggttg agttatagaa
    30121 ggatttctga ttttataaaa aatttgccta aaagacctat agttacagca tttactgcaa
    30181 cagcttcaga agaggtaaga actgatataa taaatttact gtgtttagaa aatccagatt
    30241 attatataac tggatttgat agagagaatt tatcaataaa tatagtaaaa tcatctagta
    30301 aaaataagta tattttagat tatattcaaa atcataaaaa tgagtctgga attatatatg
    30361 ttgcaactag aaaggaagtt gaaaatatat ataatggttt atctaagaga aatatatctg
    30421 tgtcaaaata tcatgcaggt ttatctaaaa atgaaagaag taaaaatcaa gaagatttta
    30481 taaatgataa tgtggatata atggtagcaa caaatgcatt tggaatgggt attgataaac
    30541 ctaatataag atgggttata cattacaata tgcctcaaag tatagaaaac tactatcaag
    30601 aaataggtag agctggtagg gatggagaaa aaagtgagtg tgtactttta ttttctccag
    30661 gagatgtaca tatacaaaaa tatttaattg atattggagt tgaaaatcct gaaagaaaat
    30721 tatttcagca taagaagctt caatacatga ttgacttagt ttacagtaat tcatgctata
    30781 gaaagacaat attgagttat tttggagaaa attatataga caattgcaat aattgtagca
    30841 attgtttagt agagggtgaa atagtagata aaactataga tgcacaaaaa gtcatttcat
    30901 gtgtagctag aatgaaaaga agttatggtg ttactactat tgtagatgtg cttagaggtt
    30961 ctaagaataa aaagatttta cagcttggat taaatacttt gtctacatat aacattatga
    31021 gagattattc tagtgaagac ttaaagaatt ttataaatac attggtttct catggttttt
    31081 tagatttagt tgagactcta ggcaatggaa atagaggtag ttttcctact ataagattaa
    31141 atgagatgtc tatgaaagtt ttaaaaggtg aagtgaaagt agagtttaaa gaaattgtta
    31201 tgacaaaatc attggaagta gaggatgagt tatataatgc tttaagagaa cttagacatt
    31261 caattgcaag tgaagaaagg atagctccat atatggtatt tggagatggt acacttagag
    31321 ccatgtctag tagttatcca attaataaag aagaaatgct agatatctct ggagtaggag
    31381 aaattaaata ccaaaaatat ggtagagatt ttgaagcagt aattaaagaa tatgtcgaaa
    31441 aaaataatat aaataaaaaa atagatgaaa aaaatgtcga ttcaaagaac gttaatagtg
    31501 atttcttaga ggttaataca gataaagttt tatatgaaaa actgttagtc attagagaag
    31561 aatatgcaac aaaggaaaaa ttacctgcat atatgatttt agccaataag gctttgaaag
    31621 aaataagtgg aagatatcca cttgatgaag aacaattaaa agatatttct ggcataggtg
    31681 gagtaaaatt tgaaaaatat ggagctagta ttttggcaga ggttaataaa tatgtaaaag
    31741 aaaataatat taatgttatt tgggagaaaa agggcaaaag gaagttaata ttagatggag
    31801 aaagtagaaa aaataatgaa atagcattag atttgctaaa tcaaggaaaa gatatacaaa
    31861 gtatcagtca agaaatagaa gtttcactgt caaccattat tggatatgtg catgactatg
    31921 taaaggatgg acatagtata tcatttgaat taggactaga aaatttttat aatgaagata
    31981 aaaagaagat gattttagaa gtttgtgaaa aagttggcta tgataattta aataacataa
    32041 aaagaaaact tccagattca ataaagtatg aaaatataag agctgtgata cttgactctt
    32101 atcttgaaaa cttaaaaaat aatattatag cagagtaatt tttaacttaa taaaaaataa
    32161 aatttgtatt attgtgttga actaataatt ttgctgtgtt atactgaatt tgtaaaaata
    32221 atgaaaaatt agagctaggg gcgctagaat tggctagctg agagataaag ccgtaactta
    32281 caaaccctaa taacctgatc aagataatgc ttgcgaaggg aagcttttta atataatttt
    32341 attatataaa taggcaaatt attcaatgtt aggagaaatt tgtctaagtg tatatacgat
    32401 tataagagtg ctttccaaac gaggaaaagc acttttttta ttttcaaaaa ctataacttt
    32461 aatcttggag gattgtaata tgaactatac aacacaaatg gatgctgcta gaaaaggcat
    32521 aataactaaa gaaatggaaa tcgtttctca aaaagaacag gtagatgtaa atgaattaag
    32581 agaattaata gcaaatggac aagttgttat accagctaat aaaaatcata aatctttaag
    32641 tgcagaaggt gtaggtaaaa atttaagaac aaaaataaat gtaaatctag gaatttcaag
    32701 agattgtaag gatatagaaa aagaacttga aaaagttaga gtagcaatag acatgaaagc
    32761 tgaagctata atggatttaa gtaattatgg caaaactaga gaatttagag aaaaggttgt
    32821 tgaaatgtca cctgcaatga taggttcagt acctatgtat gatgcagtag gatatttgga
    32881 aaaagaatta aaagatataa cagaagaaga gtttttaaat gtaataagac aacatgctat
    32941 tgatggagta gactttatta ctatacatgc aggtcttaca agaagtgttt gccaaaaaat
    33001 aaaaaatcat gagagattga ctcatatagt atctcgtgga ggttctttat tgtttgcatg
    33061 gatggaatta aataataagg aaaatcctat ttatactaat tttgataaaa tattggatat
    33121 atgtgaagaa tatgatgtta cattgagttt aggagatgct tgtagaccag gttgtataaa
    33181 ggattctact gatggtgttc aaattcaaga acttgtagta ttaggtgaac ttactaaaag
    33241 agcatgggaa agaaatgtac aagtaatgat agaaggtcct ggacacatgg ctattgatga
    33301 aatagaagca aatgtagtat tagaaaagag attatgccat ggagcacctt tttatgttct
    33361 tggaccattg gttacagata tagcaccagg ttatgaccat ataacaagtg caataggagg
    33421 agctttagca tgtgccaaag gagtagattt tttatgttat gtaactccag cagaacattt
    33481 aagattgcct aatttagatg atatgaaaga gggtataata gctgcaaaga tagctgctca
    33541 tgcaggagat atagctaaaa atgttaaagg tgctcgtgaa tgggataata agatgagtaa
    33601 agctagagct gatttagact ggtgtgagat gtttagactt gcaatagacc ctgaaaaagc
    33661 taagagatac agagatgaat caactcctac tcatgaggat agttgtacta tgtgtggaaa
    33721 aatgtgctca atgagaacag ttaagaaaat attaaacaat gaggaactta atctaatata
    33781 atttttggct agtatgactg gaataaaaag aagctgaaaa gaggaggtga tttagtgaag
    33841 gttaatggga aggaaattga atttgaaaaa gatttgactg ttatagattt attaaataaa
    33901 tataatttaa aaagtgatag agttgtagtt gaagtgaatt tagagataat tgaagaatct
    33961 aactacaata catatgtatt aaaagacgag gacattgtag agcttattag ctttattgga
    34021 ggtggttaaa ataaaaatat ttgtaaatga attattaaca gatgttgagg aaaatacaac
    34081 tgcttataaa gttagagata gctataacaa taaatgtgat gtattggtgt taaatggata
    34141 tccaattaaa acagatatgc ccctttttga aaatgataaa ttaacactta tacaaaaagg
    34201 agttaagcca tcacttgatg aattagaaag cttgttgatt gctagacata ctccaaatat
    34261 acataataaa ttaaaaaaag ggaaagttgc tatattggga ttggggggac ttggttccaa
    34321 tatagctatc tctttagcta gaataggagt gggagaactt ttacttatag attatgatgt
    34381 tgtagaacca tctaatttaa atagacagca gtattttata gatgatatag gaaaactaaa
    34441 aactgatgcc atgatagaaa atataaaaaa aataaatcca tttataaaat taaataaacg
    34501 agatattttt ttaaacaaga acaatatgga tactattaag gattcagatt taataattga
    34561 ggcctttgat gatgcttcat gtaaagcaca ggtatgtaat tatgtattaa taaatttaaa
    34621 agataaatat ttaatagcat catctggtat ggcaggttac tatgattcaa atataataac
    34681 tactaagaag ataaaagaca ggttttatat atgtggggat tttgtgaatg aagctaaatt
    34741 tggtgaaggg cttatggctc caagggttgc catctgcgca aatcatatgg caaatttggc
    34801 aacgcaaata cttatagata tgtaaatatt aaaattagat ataaaaaaga ttgaatatta
    34861 aaaaaattta acgtggaggg aaataatatg gataaattga tacttggtgg acatgaattt
    34921 aatagcagat tattggttgg aacaggtaaa tatggttcaa ataatatatt gcctgaggta
    34981 ataaaagaaa gtggaagtga aataataaca atggcactta gaagagtgga tttagacaat
    35041 aaacaagaaa acatattaac gtatattcca aaagagatga caatacttcc aaatacatca
    35101 ggagctacta atgcagaaga agctgtaaga atagctagaa tatctagaaa aatgggatgt
    35161 ggagatttta taaaaataga agtaatatca gatactagat atttacttcc tgataatgaa
    35221 gaaactataa aagctacaaa gatacttgct gatgaggggt ttatagttct tccatatatg
    35281 acaccagacc tttatgctgg aagaagactt atagaagcta atgcagcggc agttatgcca
    35341 cttggagctc caattggttc aaatagaggt cttcaaatga aagaaatgat aagaataatg
    35401 atagatgagt tggatatacc aataatagta gatgcaggta ttggaaaacc atctcaagct
    35461 atggaggcta tggaaatggg agctgatgca gtacttgtca acactgctat agcttcagca
    35521 ggagaccctg tacaaatggc tagagcattt aaattagctg tagaaggtgg aagagaagca
    35581 tatattgcaa aaacaggaaa tgtgtcagaa tttgcaaatg catcttctcc tttaacaggt
    35641 tttttaggca atttatagaa agtggtgatt ggtgatgagt ttttatgatg taatagaaaa
    35701 atatagggat tttgattttg atggatattt aaataatgtt actgataatg atgttttaag
    35761 aagtttatca aaagacaagc ttgaagattt tgatatatta aatttgcttt ctaaaactgc
    35821 agtaaaacat ttagaagata tggcacaaaa agcacataaa ttaagtgttc aatactttgg
    35881 taaaacagta tgtttatata caccaatgta tatagcaaat tattgtgtta atcaatgtgt
    35941 ttattgtagc tataatataa agagtggaat aaaaagaaaa aaattaacta tggatgaaat
    36001 tagagaagag ggagaagcaa tttcaaaaga aggtttcaaa catctacttg ttttgactgg
    36061 agaaagtagt tttcactcaa gtgtagaata tataggtgag gctatagaaa tattaagaga
    36121 gaaatttccc tctataggta tagaagttta tccaatggaa gtagaagaat ataagtacat
    36181 tgtagataaa ggtgttgaag gattaacagt atatcaagaa acttatgacg aggaaatata
    36241 taagagagta catataaaag gcccaaaatc aaattataaa tttagactag atgctcctga
    36301 aagaggggca aaagcaggta tgaggacttt atcaataggt gcacttttag ggctaaatga
    36361 ttttagaaaa gaaacttttt ttacaatatt gcatgggaaa tacttaaaaa cgaaatatcc
    36421 tcatattgag ctttcatatt caacaccaag aatgagacct tttaaagggt gttttgagga
    36481 attggtagat ataagtgata ctgatttagt tcaagctatg gtatgtatga gattatttga
    36541 ccctcatgca gctataaaca tatccactag agaaaattta gaaatgagaa gtcatataat
    36601 acctttagga gtaactaaat tgagtgcagg tgtatctact gatgtaggag gtcattctca
    36661 agatgaacat gatactgctc aatttaagat aaatgatgaa agtactgtta aagatgtgga
    36721 gaaaatgcta aactcaattg gttatcagca tgtgtttaaa gattgggaaa gattttaaat
    36781 gtatctgata acaaacagaa aattatgtag tgaggaacga tatttagaag ttattaaaga
    36841 gtctatattg agtggtgtag aaaatataat aataagagaa aaggatttag aatatcaaga
    36901 gctaagaaaa ttatatatga aaataaaaac taaaatcaat tgtatagatt ttcaagagca
    36961 aatatcagat gaaagtttga aaactaatat aaatcaaaaa gagtttagaa ataaattcaa
    37021 ggttaatttt ataataaata gtaacattga attttttgaa aaagtggatt gtcaaggtat
    37081 ccatcttcct tttaaattat tcttgaattt aattgaaaat aagtataatt ttaatgaaaa
    37141 taaaatacta ggattatctt tacataaagt agaggaagta gattatttag aaaaattaat
    37201 aagaaatcaa aatataaaaa tagattacat aacattatca catatatatg aaactaaatg
    37261 taaagaagga ttaaatccaa aaggaattga gcttttaaaa gaagctaaaa agattactga
    37321 tattaaaata attgcacttg ggggaatttt accatctaat gtcaaggaaa cattaaaata
    37381 ctgtgatgat tttgctataa tgtcaactat aatgaggtct aaagatatta aaaaaaccat
    37441 atcaaattat aat
//
LOCUS       DAEMHZ010000024        37430 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000024 DAEMHZ010000000
VERSION     DAEMHZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.024"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(141..335)
                     /locus_tag="KPK72_001124"
     CDS             complement(141..335)
                     /locus_tag="KPK72_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488360.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(644..2173)
                     /gene="lysS"
                     /locus_tag="KPK72_001125"
     CDS             complement(644..2173)
                     /gene="lysS"
                     /locus_tag="KPK72_001125"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG2488361.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2188..2667)
                     /gene="greA"
                     /locus_tag="KPK72_001126"
     CDS             complement(2188..2667)
                     /gene="greA"
                     /locus_tag="KPK72_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG2488362.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2776..3744)
                     /gene="dusB"
                     /locus_tag="KPK72_001127"
     CDS             complement(2776..3744)
                     /gene="dusB"
                     /locus_tag="KPK72_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG2488363.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3881..4651)
                     /locus_tag="KPK72_001128"
     CDS             complement(3881..4651)
                     /locus_tag="KPK72_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG2488364.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4670..5260)
                     /locus_tag="KPK72_001129"
     CDS             complement(4670..5260)
                     /locus_tag="KPK72_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2488365.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5444..6412)
                     /locus_tag="KPK72_001130"
     CDS             complement(5444..6412)
                     /locus_tag="KPK72_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG2488366.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6405..7382)
                     /locus_tag="KPK72_001131"
     CDS             complement(6405..7382)
                     /locus_tag="KPK72_001131"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG2488367.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7697..9667)
                     /gene="ftsH"
                     /locus_tag="KPK72_001132"
     CDS             complement(7697..9667)
                     /gene="ftsH"
                     /locus_tag="KPK72_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2488368.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9701..11089)
                     /gene="tilS"
                     /locus_tag="KPK72_001133"
     CDS             complement(9701..11089)
                     /gene="tilS"
                     /locus_tag="KPK72_001133"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG2488369.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDNFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDCIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11272..11700)
                     /locus_tag="KPK72_001134"
     CDS             complement(11272..11700)
                     /locus_tag="KPK72_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG2488370.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11717..12523)
                     /locus_tag="KPK72_001135"
     CDS             complement(11717..12523)
                     /locus_tag="KPK72_001135"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG2488371.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12725..13210)
                     /locus_tag="KPK72_001136"
     CDS             complement(12725..13210)
                     /locus_tag="KPK72_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG2488372.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13261..13920)
                     /gene="spoIIR"
                     /locus_tag="KPK72_001137"
     CDS             complement(13261..13920)
                     /gene="spoIIR"
                     /locus_tag="KPK72_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG2488373.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14065..14745)
                     /locus_tag="KPK72_001138"
     CDS             complement(14065..14745)
                     /locus_tag="KPK72_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2488374.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14729..15619)
                     /locus_tag="KPK72_001139"
     CDS             complement(14729..15619)
                     /locus_tag="KPK72_001139"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG2488375.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15737..17287)
                     /locus_tag="KPK72_001140"
     CDS             complement(15737..17287)
                     /locus_tag="KPK72_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2488376.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17381..17647)
                     /locus_tag="KPK72_001141"
     CDS             complement(17381..17647)
                     /locus_tag="KPK72_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG2488377.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17795..18655)
                     /gene="yabG"
                     /locus_tag="KPK72_001142"
     CDS             complement(17795..18655)
                     /gene="yabG"
                     /locus_tag="KPK72_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG2488378.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18739..19677)
                     /locus_tag="KPK72_001143"
     CDS             complement(18739..19677)
                     /locus_tag="KPK72_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG2488379.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYKEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19787..19948)
                     /locus_tag="KPK72_001144"
     CDS             complement(19787..19948)
                     /locus_tag="KPK72_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488380.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19958..20455)
                     /locus_tag="KPK72_001145"
     CDS             complement(19958..20455)
                     /locus_tag="KPK72_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2488381.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20570..21280)
                     /locus_tag="KPK72_001146"
     CDS             complement(20570..21280)
                     /locus_tag="KPK72_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG2488382.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21456..21986)
                     /locus_tag="KPK72_001147"
     CDS             complement(21456..21986)
                     /locus_tag="KPK72_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488383.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKPIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22003..22233)
                     /locus_tag="KPK72_001148"
     CDS             complement(22003..22233)
                     /locus_tag="KPK72_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488384.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22248..23642)
                     /locus_tag="KPK72_001149"
     CDS             complement(22248..23642)
                     /locus_tag="KPK72_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2488385.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23768..25021)
                     /locus_tag="KPK72_001150"
     CDS             complement(23768..25021)
                     /locus_tag="KPK72_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2488386.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25072..26226)
                     /locus_tag="KPK72_001151"
     CDS             complement(25072..26226)
                     /locus_tag="KPK72_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2488387.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(26786..27355)
                     /locus_tag="KPK72_001152"
     CDS             complement(26786..27355)
                     /locus_tag="KPK72_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2488388.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27558..27785
                     /locus_tag="KPK72_001153"
     CDS             27558..27785
                     /locus_tag="KPK72_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2488389.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(28004..28747)
                     /locus_tag="KPK72_001154"
     CDS             complement(28004..28747)
                     /locus_tag="KPK72_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2488390.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28915..29787
                     /locus_tag="KPK72_001155"
     CDS             28915..29787
                     /locus_tag="KPK72_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2488391.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDVYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29787..30452
                     /locus_tag="KPK72_001156"
     CDS             29787..30452
                     /locus_tag="KPK72_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2488392.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30473..31330
                     /locus_tag="KPK72_001157"
     CDS             30473..31330
                     /locus_tag="KPK72_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2488393.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31486..34032)
                     /locus_tag="KPK72_001158"
     CDS             complement(31486..34032)
                     /locus_tag="KPK72_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2488394.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPVEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     TEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34032..34718)
                     /locus_tag="KPK72_001159"
     CDS             complement(34032..34718)
                     /locus_tag="KPK72_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488395.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34801..35790)
                     /locus_tag="KPK72_001160"
     CDS             complement(34801..35790)
                     /locus_tag="KPK72_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488396.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35777..36463)
                     /locus_tag="KPK72_001161"
     CDS             complement(35777..36463)
                     /locus_tag="KPK72_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488397.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36963..>37430)
                     /locus_tag="KPK72_001162"
     CDS             complement(36963..>37430)
                     /locus_tag="KPK72_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2488398.1"
                     /translation="LGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEI
                     AKDMKELGYTFVATEGTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPT
                     KGNDSTRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT        11963 a         6939 c         4191 g        14337 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt
       61 ggtttttttt aataaatata attaaaacta atagatataa cccttgatat tacaaacaat
      121 gcatgcatat ataattttac ctattttgca attttatttt cataagaata tgaattagct
      181 ctcactctat ctgcccaaac atttatactc ttaactttat taacattagt tgtaccctca
      241 aaaatatcca ttgcatcctt acaaaaatat atcataacat caagaaccaa aaatccaaca
      301 caaaataaac ataaaaaatt aattacttga aacatcagac attcccccaa taatttttta
      361 tatatagtat tctcataaat tattcttttt accttctcat aaattattct aaatattttc
      421 ataataaatt actttcaatc atattttcta aatataaagt ctcaatcaac aaaaactaat
      481 atcaatttct aacgtttgtt ggtaatttat aaattaaata tctaaaagaa tctttgaagc
      541 atataaaaag cattctacac tgacatttta aataaaattt agctatttat tgataattta
      601 atagctaaac tttatttaaa acttagtaga atgctaatat ttcttagttt tcttcttctt
      661 tatttggatt gttatcaata ggtttcatag tcgggaataa taatacatct cttatagatg
      721 gagagttagt taataacata attactctat ctatacctat tcctagtcct cctgtaggag
      781 gtagacctac ctctaatgct tttaagaaat cttcatccat ttcaaaagct tcatcatcac
      841 ctaattctct ttttctcaat tggtctataa atcttccttt ttggtcaata gggtcattaa
      901 gttcagaaaa tgcattagct aactcccact tattagcaaa tgcctcaaat ctatcagttc
      961 ttcttgggtc ttcaacattt ctcttagata atggagatac ttcaactgga tgatgagtta
     1021 taaatgttgg ttggattaat ttatcttctc caaattcttc aaagaaagca tttattactt
     1081 ctcctcttct cattccaggt tttatctcta tacctttttc tctagcaact tctagtgcct
     1141 cttcatctgt atttatagta gaaaaatcta caccagaata ttctttaaca caatcttcca
     1201 tactcattct tttccaaggt ggtgtaaagt ttatttctgt tccttgatag ttaactatca
     1261 tacttcctgt agcaacttca gccatatgtg caataacatt ttctgttatt tccatcatat
     1321 cagtatagtc tgcatatgct tgatataatt ctattgcagt atattcagga ttatgtttta
     1381 aatccatacc ttcatttcta aacattcttc ccatttcata aactttatca aatccaccaa
     1441 caataagtct ttttaaatac aattcattcg ctattcttaa atacattggt atatgtaaag
     1501 tattatgatt agttataaat ggtctagcat ttgccccacc tgctatagta tttaatattg
     1561 gagtctcaac ttctaaaaat cctctatcat ctaagtatgc tcttaatgct tttaatgctt
     1621 ttgttctaat taaaaaagca ttttttactt ctggatttac tattaagtca acatatcttt
     1681 gtctatatct tagttcttgg tcttttaatc catgatattt ttctggtaaa acttgtaaag
     1741 atttacatag taatactaca gatttagcct ttacagatat ttcacctttt ttagttttaa
     1801 agatttctcc ttcaattcca actatatctc caatatcata tgttgaaaat acactatatt
     1861 cttcctctcc tatagcatct agtcttacat aagattggat tcttccctct tggtcttgaa
     1921 tatctataaa gccagctttc ccttgtattc tcttgctcat tatacgtcct gctatttttg
     1981 tagttttacc ctcaagagaa tcaaagttat cttttatatc catagaataa tgagttctat
     2041 catatctact ttctttaaaa ggatctctac ctgactcttg taattttttt agtttatctc
     2101 ttcttacttg aagtacttca ctaaggtctt cttgaatttg cgcttcttca ttgctttgtt
     2161 gattattttt catttttttc ctccaaatta tcttcttatt tctaatattt caaacttagc
     2221 aactccatca ggaacttgca cttccacaac ttctttcgca gctctaccta aaagtgcttt
     2281 ccctacagga gattcgtttg atattttacc atcatatggg tctgcttctg tcgaaccaac
     2341 tatagtatat tcaacctctt catcaaattc taaatcctta actttaacta ttgatcctat
     2401 tgtaacaaca ttaaggtcta ttttgctttc atctatgata acagcttttc taaccatatt
     2461 ttctaatttt aaaattcttt cttcaacttg tgcttgttcc tttttagctt catcatactc
     2521 agcattttca gataaatcac caaaagatat agcaactttt attctctctg caacttcttt
     2581 tctctttaca acctttaagt tttctaattc ttcttctaat ttatcatatc cctcttgtgt
     2641 caataaaaac tctttatttt cttccattat aaatacaact ccttcacaaa tttcttagga
     2701 attttcctaa ttatatttta aaaattttat aatctttttt taatttatgc gctatattat
     2761 aagtcactat tatttttatg tcaaggagtc ttccatatag tactccaatt tcgacacaac
     2821 ttcttgatag tcttctattt tgttaatttc atcccttaat tttgcagagt ttctaagtcc
     2881 ttttaaatac caagcgatgt gttttctcat ttcccttaca gctacatact ctccatgttc
     2941 ttctactgca agttttaaat gcttaatggc agtacttatt ttttcgttaa gagttggctc
     3001 tggcaaaatc tcgcctgtat tcatataatg gtttattctt ttaaatatcc aagggttacc
     3061 ttgagcaccc ctacctatca ttatagcatc acaaccagtt ttgtctaaca tatttattga
     3121 atcttctatt gtaaatacat caccatttcc tataacaggt atactcaaat ttttcttaat
     3181 ctctgcaatt atatcccagt ctgctttgcc tgtataaaac tgttctctag ttcttccatg
     3241 tattgctaaa gcacttattc cacaatcctc tgctatcttt gctatttcaa cagcatttac
     3301 actattatca tcccagcctt ttcgaatctt taaagtcact ggcttttttg agttcttcac
     3361 tactgccctc aatacctctt ctgcaagctt aggatttttc ataagtgctg aaccatctcc
     3421 atttttaact acttttggag ctggacaccc catatttata tcgagaatct catttgaata
     3481 atcattcatt atctctgctg ctcttcccat aaactctggt tctgaaccga atatctgaac
     3541 tgctacagga tgttcttcct cttctatctt aagcattttt ttagtatttt catcatcata
     3601 gcaaagtgcc ttaccattta tcatttctgt atataaaagc ccacatccct gttctttaca
     3661 aattaatcta aatggaaggt ctgttacacc tgccataggt gatagaaaaa ctttattttt
     3721 caattctaaa tttcctattt tcattgtttt tctcctattt ttgcctttat tttgtgattt
     3781 aactttaaat tcttatttcc tcattataat ataccacata tagctagaaa tctatcataa
     3841 aaagggaatt ggatatataa cccaattccc tgtatattaa ttattcttgg tttttttcat
     3901 atattattct caatccttct agtgttaaaa aaccatctac ataatctata cttttcgtct
     3961 ctgaagcaat cagtttagct aatccacctg tagctataac cttaacatca tcacaattca
     4021 attctttctt catcatactt attattttgt caactaaacc aacatatcca taaattattc
     4081 cagattgcat ggctgataca gtactcttac aaatagtcat acctggctta gctaattcta
     4141 ctctaggtaa tttagacgca ctttgaaata acgcctcact agatattttt attcctggtg
     4201 ctattgttcc acccaaatat tcaccttttt cagagatagc acaaaatgta gttgctgttc
     4261 caaaatcaac aagtatactt ggtgctccat acttttctat ccctgctaca gcattaacta
     4321 ttctatctgc cccaacttgt tttggattat catattttat atttagacct gtttttatgc
     4381 ctggacctac tattaatggc tgttttttac agtactttat acaaaagttt tcaagagaat
     4441 gcataacatt cggaactaca gatgatatta taacatcatc aatatcactt atattcacat
     4501 tatcataatc aaataggtta cttatcagga ttccatattc atcagacgtt ttttccctat
     4561 ctgttttaat tctccagtaa ttaactaatt tgtcaccttt atatatacct aaaaccatat
     4621 tagtatttcc aacatcaaat actagaagca tatgtcatta accttctttc tattttttgc
     4681 ttttcttaag tacagtggca acagcagcta ctactaaagc ccctactatt gcttctggca
     4741 ccccatttgc tgcggcaaga gccattatta cttttcctgc tgaaccactt ttacccattg
     4801 catgaagata cttgtcagca tataagacat atatcattcc taaaactcca gctgtattta
     4861 acagagaacc tattgcccct gtaattaaac ctgatatgta taccttattt gtagccttaa
     4921 ttattaactg atatacataa taagctaata cacctatcat aactcttggt aatattgaaa
     4981 ctaatggatt tataaaaaca aaagatgtta ctgttgggtt tgttatagca tttaagagac
     5041 ttgatatccc aaatataaat cctattgctg ccccaactat tggtccttcc ataatagctc
     5101 caataataac tggtatgtgc attatagttg cttttgtagg tccaactgga ataaatccca
     5161 atggagtcat agatagcata atagaaatag ctgatagtac tcctattata gtcatctttc
     5221 ttacatttac ttttcttact gatgcacttg tgttcatcat aaaaaataca cctccgttcc
     5281 agctcattaa gatgccggac tacttaattt tttaattttt ttcgtttttt tcttataact
     5341 atgagtcaag ctaccattgt aggatgcccg ctctaacaag aataatttta tcaagatgag
     5401 ttgccattgt caacaatttg acaagaaaaa tatgctaatt tcatcatatt tctcgttttt
     5461 ttactatatc tttatatgcc attatatttt ctacactatc cgcttttttt actgcattta
     5521 ttatactttt ttccacaacc tctacagcca atgaacctac taatgttata tctgcttcta
     5581 tttctcctgt tgatattgtg aatattgtat ctccatcatg aggtgtatgg ataggaaaaa
     5641 tagacttagc atatccatca tgtcccattt gagatatctt tttacatcca gctttggtta
     5701 acttagcatt tgttgcaatt atcccaattg tagtattatc tacactaaat ccacctttac
     5761 tttcaccttg tttcattaat ttatatgtat caattgtttc tgtcttttta tcatttaaag
     5821 caccagctat aaccttacca ttttcataaa catctccaag agcatttaca gcaattaatg
     5881 cagatacaat tagaccatta tctaatttta tagaatagct acctattccg cctttcatag
     5941 catattcctg acctcttatc tttccaacag tggctccaca acctgctcca taatttcctt
     6001 gatttaaaat atcagtattt gcaacttcac aagctctata acccatcttt aaatctggtc
     6061 tacatttaaa gctccctact cctaaatcaa ataaaactgc tcctacaact ataggcactt
     6121 tacctacacc tacatcaaat ccaattcctt tttcttctaa gtatctactt acaccacaag
     6181 tggattcaag accatatgca gaacctccag ccaaaactac tgcatgaact ttttgaacca
     6241 tatttacagg gtctaataaa tcggtttctc tagtacctgg agcagagccc cttacatcaa
     6301 caccgcaagt agctccttct tcacatatta ctactgtgca cccagtaaga ccatttctat
     6361 cctcaacctg cccaaccttt aagccttcta tatccaaaat attattatac atatcctttt
     6421 tctcctctta tagaaacttc acctgaaata atttctttta tacttccatc acttctttga
     6481 actaataaat ttccctcttc atttatgtct aaacatttca ctagttctct attatctcct
     6541 tttaatacat atatattttt atctataatt gcagaataat ctctacatat ttttaaggtt
     6601 ttttctttag aattatttac agtataatct aaatataatt tttcaaattc agtcaataca
     6661 ttcctaacaa tatctactct ggataacata tacccttctt tatacaatga tgtggctttg
     6721 tcagctattt cttgttcaaa atccattatt ttaacattta ttccaattcc aagaacaaca
     6781 taatttgttc tttcaatttc agcagataac tctgttaata taccacatat tttttttcca
     6841 tttaaaataa catcatttgg ccattttatt aatgtatttg ctcctagatt gtttaaagct
     6901 ttcgttacac ttgctcctgc tattaaagtt ataaatgggg ctttgaatgg ttccatatct
     6961 ggttttaata taatactcat ccagattcct tcatgaggtt ttgaatccca tcttcttccc
     7021 aatcgtcctt tccccatatc ttgatgttca cttaaaatca cagtcccatc tacactattt
     7081 atagctatcc ttttagcatg ttcatttgtt gaatctatag tgtcaagatg ttcaatattt
     7141 tttccaataa actctgtatc taatttatag taaatattct gtgggcttaa taaatcagtt
     7201 ggattttccg ctaatcgata ccctttttta tttacagatt ctatattata tccctcttct
     7261 tttaaggctt tcatatgttt ccatatagct gcacgtgaca cccctaattg ttttgatagt
     7321 tcttctcctg aaataaattc agatttatta tttaatataa tctctattat tttatctcgc
     7381 atatttcacc tctaatatga cataatttca tgacttattt tcttctaata aataattata
     7441 cattattaat atacttttaa tactattaat tttaaatttc ctttttaatt attataatgg
     7501 ttgttttata ttttatattg atgatttata taattttaca ttttatttaa ataattttaa
     7561 aataataaaa aaactgaaag tatcaattaa attaatacat gcaaatataa tttgcttatt
     7621 aatttaatat tatactttca gttttagtat ttaatccaca tcagataata atcctaaaat
     7681 gaccaaggtc ttaaaactat ttatctttat cagatttatc ttctaaattt ttatttatat
     7741 caataacatt gtttctttct tcttcatctt tttctaattt tatagttaat tgttcttcaa
     7801 cttctttaga agaattttcc tctgtaactg cattttcaat ttcattcaga ggtaactctt
     7861 tattaaatgc ttttacaaat tgttctgcat ctaaagtttc atatataagt aaagcttgtg
     7921 ctacatactc taatctatcc atattttctt gtaatatact tcttgtctta tcatatgcac
     7981 catcaactat acgctttgtt tcttggtcta tctcaaaagc aacttcttca gaataatttc
     8041 tcttacttga aaaactatta cctaggaata cttcatcatc actgtcaaat gacattggac
     8101 caagtttaga actcattcca tactttgtta ccatacttct tgcagtagct gtaactcttt
     8161 ctaagtcatt tgaagcacct gtagatacat ccttaagtac tagctcttct gctactctac
     8221 caccaagtaa aactacaata ttttctttca tttcattttt tgtagcatag aacttatctt
     8281 caacaggaag ttgcattgta aaccctccag ctcttcctct tggaactatt gtaacttgat
     8341 gaacaggact tacttcttca agtacatggg cacaaactgc atgccctcct tcatggtatg
     8401 ctgtaagtct tctttctttc tcacttatta ctttcgattt tttagccaca cctgctataa
     8461 cctttgttat agcttcttct atagtttcca tcttaatttt cttttctctt tttctagctg
     8521 ttaatattgc agcctcattc attaagttct ctatatcagc aggagtaaat cctggtgttc
     8581 ttctagccaa gacatccatt tttacatcat cacttaatgg tttatttctt gagtgaactt
     8641 taaatatagc ctctctacct tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc
     8701 ttcctggtct aagtaaagct gggtcaagta tatctggtct atttgtagct gccatgatta
     8761 ttataccttg atttacccca aatccatcca tttcaactag aagttgatta agagtttgct
     8821 ctctttcatc atgacctcca ccaagacctg cacctctttt tctaccaact gcatcaattt
     8881 catctatgaa tatgatagct ggagcacttt tcttagcctg ttcaaataaa tctcttactc
     8941 ttgaagcacc aaccccaaca aacatctcaa cgaaatcaga accacttata ctgaaaaagg
     9001 gaactcctgc ttctcctgca actgctcttg atagatatgt tttaccagtt ccaggaggtc
     9061 ctaccataag cattcctttt ggtattcttg cacccaattc aatatatttc ttaggattct
     9121 ttaagaaatc tactacctcc tgcaaatctt ctttttcctc atctagtcca gctacatctt
     9181 taaatgtaac tctagttttt tcatcatctt tatgaacctt agcttttgat ttgccaaaac
     9241 tcataacctt tccgcctcca ccttgagact gattcataaa cacgaaccaa ataatcacca
     9301 tgaagaatat taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag
     9361 cttcaccacc aaaagttagt ttcccagcct tagcttggtc taacacctca tctgcaagtt
     9421 tatttcccat cacttctgtt ggaacatatg acttaaactt tgtatttgta tcttttatag
     9481 taccttctac agaagtttga tttacaaagt ataatcttga tatattctca tctgttaatt
     9541 ctctatatac ttcagagaat tttaaatctt ttattttttc tgtaggctta cctgagaatt
     9601 gaactatacc tactataatt ataaaaacaa gtagataaaa acctgcccct tttagcaact
     9661 tattcaatat agaagccctc ctttcataca ttgctttgcc ctataatttg ttaaaattaa
     9721 tttttaatac ttcttttgtg ttttcatcaa ttttataatt ttcactgatt ttataatctc
     9781 caacacatat tattccttca ctatctgtga ttataggaat tttacatctt tcttctcttg
     9841 ggatttttaa atctatgaat aaatctttca cttttttact tcccatagca agctttatct
     9901 tatcaccttg ccttctgctt cttattacta tacccccttt taccttatta aaatcaaacc
     9961 ctttagatga attgtctaat ttcatactct tatacctatc tatgctcatt acttgggtct
    10021 caataatttg ttttaattct tttattttaa taaacccatt actaggtaca ttgtaataaa
    10081 attcaatttc ctcattaaca atttcctcat tcgttaatat tatacagtct ttttttctat
    10141 aaacaaatat atttcttgga agagttaaca ttttatttac ttttgaatta tcttctaaag
    10201 acattatatc ctctatatgt ctttggtcaa caaaattagt atctcctagt atatgtttta
    10261 tggaatttct aagaactcta actttgatag catcatgtaa gcaaacaaaa ttgtctaaat
    10321 ttatctctac aaagcctttt tcttttatat tcgaaacttc tctaaattta gcttctgcct
    10381 ctttctcaat ataatcgtta tcacttttta aactattgct catcctcact atagattcta
    10441 ttacatttga attaaaatta tccttcatat atggtaaaag gtctaacctt atcttatttc
    10501 tagtgtatat attttctaaa tttgttttat ctatcctcgg atttaaatta taagcttcac
    10561 aatactcctc tatctcattt ctttccacgt ctaatatggg tctaattatg caattatctc
    10621 taatataatc aattcccttt aaacctttta aacctgttcc tctcatgata cgcatcataa
    10681 ccgtttcggc ttggtcattg agattatgac ctattgctat tttattggct ttaatcttat
    10741 ccttaatttc ataaaacatt tcatatctta gcttccttgc tccttcttct aatgataatc
    10801 cctcattttc acaatatttt ggtacattaa tagactttac aaagaatata attcccatat
    10861 cctcacaaag tttagataca tataatgcat ctttttgagc ttctatccct cttatttgat
    10921 ggtttaaatg tgctgcatat atttctatat taaaatcttt ttttaatcta tttagtacat
    10981 gcaataaaca aactgaatca ggtcctccag aaagacctaa aactatttta tcgccttttt
    11041 gtattaaatt atgtttattt atagtactta gtaccttatc aaatatcata tttatccctc
    11101 ttacacttag ttttattcac ttttaagtat actttctata aaaaaacgct aaacccttgt
    11161 atcaatttta agaaattaaa gtttaatata aatataataa taccaaataa attaaataaa
    11221 aggtagtttt ttcactacct ttataaaatt tctggaaaat agccatagtt attatttagt
    11281 acctatgtat atatctattt tattcctacc taaaaataaa tcttgtacct ttatattata
    11341 gtctattttc aattttatgt gttccccttc ttgtatattt atatctaatt ctcttgtatc
    11401 aatatcaatt aataaagtac cttgtggggt cacatacttt gtagtactac tttctccaca
    11461 tttaaacagc attgttgagt taatagcacc aaactttttg atactaactt gatttttaag
    11521 tatctttata gtattagtta tttctatatt ttcttctttt tccttgtaaa taacatatat
    11581 tccatcattt ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc
    11641 tttttcatca tattgtctag tatttatact tatttttaca ctcaacgatt tttcctccat
    11701 agattcttca tataaactag tatttttgta tttctacttt ttgaatctta tcaatttttt
    11761 tctttaagaa ctctcttgct atatctgaaa atttttcagg tatatctgat acataatatt
    11821 gataagttcc atgaatttct gtattgttta taatattttg cacctctaaa atctttttta
    11881 aatcctttgc agtctcttta gctggattaa caagttttat atgctctcca acttcttcac
    11941 ctattgttct ctttaaaata ggataatgag tacatcctaa cactaaagaa tctatatttt
    12001 tttctttcaa ttcttgtaaa tatatctttg ctgtcaattt agccacttct gtatttgccc
    12061 atccttcttc aacaataggt acaaataaag gacatgcttt attaacaatt tctatactct
    12121 catctatttt agatatttcc aagttgtatg cttttgaact tatagttcct tcagttccta
    12181 ttattcctac aattttgttt tttgtagaac ttacagctgt tctagctcct gcttctataa
    12241 ctccaattat cggtatatca tatttttctt gagcttcttt taagcttctt gcagtagcag
    12301 tgttacatgc tataactata gcttttacac cttttgtttt taaaaagttt atagcttgaa
    12361 aagtatattt aattatagtt tctttggatc ttgaaccata tggtattctg gcagtatctc
    12421 caaagtatat tatatcctca tttggtaata tcttcattat ctcttttaga actgttaatc
    12481 cacctaatcc cgaatcaaaa actcctattg gtttattgtt catctttata caacacctac
    12541 tttacatttt tgtgtttaac aacaacttat ttatcgctaa aatttatatt tatattcata
    12601 attaatatat tattacctaa atgattatta tagtttattt tacacaaaaa ataaaggagc
    12661 tatcttaaat tagaaaataa tttctacaaa aagacaactc ctttcactac ttaaaaattt
    12721 aaatttattt tgcaaacaca tgatttccta ttctcatata aggattcaat ctatttatcc
    12781 atttgcaagt ggctatttct ggattccaaa aatatactgc tttattagtt gggtcagttc
    12841 cataaagagc ttcctgagct gctttacgtg ctgagtctgt tggtgccatc tttattgaac
    12901 catcaagcac aactgagaaa gcattttttt gatatatgac ttcttctatt gtgtctggaa
    12961 atctagggtc caatactcta tttattacta cagctgctac agcaacctgt ccctcatagc
    13021 tttctcctct agcttctcct gcaaccaact tagataataa catcaaatca ttatcagata
    13081 aatttattac ttctttcttt gctatatttt tttcttgttt gtttactttc ttaatatttt
    13141 ctgttggttc atatgcaaat acctctttaa taggcataaa tataaaagtc aaagctaata
    13201 gtgtagccat cagtttactc cacctgtcca tgattaaaac cactccttac attcttattt
    13261 ttaaaatttt ttctctaaat ccttgactac ttcaagtact ttaaacttaa tttttactct
    13321 acttgttttt tttggtgtat cttgttttat taacttatat tctttatcag ttaaaacttc
    13381 cttcaactta tcatctgtag atttatctat cactccatta gattcatcaa caaaacatag
    13441 tggagggaac ataacacacc accagttttt tccttcacct ttcccaatga taatctttaa
    13501 tgctttatat tcaccagctg gaagaactat attagaatat tgtttagtag ggaaattgct
    13561 atattgtata cctaccttta cactgtaatt atatccattt ttaagaataa tatttttact
    13621 tatttcttcc agactactat attccttttt tataatagct tcactttctt ttatactttt
    13681 tgaattttga agctttggct gtaaatattt tattacttca tctctaactt ttagtttaag
    13741 ttcttggtct tcatctgtat tgctgtttgc tataacatga aatcttatta atttatcttt
    13801 ataatctttt gatatattgt ctactttttt tacctcacca tttattacta ttgtcattat
    13861 tgatatgact gatataagac ttaatattaa tagtcccaat ctaatcttaa tttttctcat
    13921 tgaaaattcc ccctcaagat aaatttatat catcaattat gtccaattaa atttaattta
    13981 ttcataatat tttttaatac taataaaaaa agctatctta aaatgattac aatcatttta
    14041 aaatagcttt ttaaacctta tattttataa gtctttacta atcatagcct tatctatcat
    14101 aaactgctct gctttttcta tatgctctgt agcatacttc ttggatagct cactatcacc
    14161 tttaattata gcatcaagta aatatttatg ctcttcaaca atattatgtg atgtatcata
    14221 agcagacata taggtaactc taaatctata tacttgttcc cacaatgagt ttattaattg
    14281 gattaatttt ttattattag ttgctttaaa tatacattca tgaaattcta catcaagttt
    14341 taataacgca tctacatcgg attccaaagt gcttttttta aattcttctg atatatcttt
    14401 aagcttttta atatcttcat cactaattct ttcagcggca agatatgctg ctaagccttc
    14461 taggcttgct cttacttcta atacatccat aatatccttt aatgacatat ttgccacata
    14521 cgcacctttt ctaggcaaca taacgactaa accttctaat tcaagctttc ttattgcctc
    14581 tctcactgga gtcctactta ctcctaattg ttctgctagt tgcacttcca ttagcctttg
    14641 accaggcttt aattttcctt ctaagattgc ttctcttaaa ttctcaaaga ctacatctct
    14701 taatggcttg taattatcca agttcagttt agttaaattc tccacaaatt tcaacccctt
    14761 tttgactgct attaacaaca tacacctgtt tatatttttt taataattct ttttttccaa
    14821 ttagagcatc ttctttattt ttaaatagtc caaaaactgt aggaccactt ccactcatca
    14881 tagaacctaa agcattattt ttcatcatta cttgttttat atcacttatt tctttgtgtt
    14941 tacctatagt aacattttca agaatattta ccatgctttc tgatacagct tttatatctt
    15001 cagattttaa acactctatc aaatatttat tatttggtct ttttttaata ttattcaaat
    15061 ctaatccttg ataaacttct ttagtagaaa cgaataagtc tggtttgcat atcaatatat
    15121 ttgtatcaca tggtaaacct ttgatgtttg ttagcttttc tccaatacct tgagctaaag
    15181 caggtcttcc tgatatacaa aaaggaacat ctgcgcccaa attaagtcct atttctttta
    15241 attcatcttc agataatcta agttcccata ggtgatttaa acctactaat actgcggctg
    15301 cattagaact tcctccagcc atccctgctg ctacaggaat atttttttct atgaatattt
    15361 ctacaccttt ttttatataa aatttatttt taagtatttt ggcagcttta taaacaatat
    15421 tatcttcatc taaaggaata tctaaactag tgctttttac ctttatttca tcttcgtcta
    15481 attcttttat ctttactata tcgtataaat ctatcgtttg cattatcatt tcaacaaaat
    15541 gataaccgtc ttgccttttt cccaagacat caatggataa atttattttt gctctactct
    15601 ttagtcttat aaaatccatt ctatcctcct tgtttaaaga ataatgcatc actaacaata
    15661 atattatact acaaaaaata aaataatagg gtattataat accctattat acttaattac
    15721 tttaaatttt ttaatattaa tctactaata cgactttttt ttctaaaata agacacttac
    15781 caggcttaat tggttcatca agttttatat cattaagttc tgcaatttca ttttctgttg
    15841 tattgtattt ttttgctata ttccagaaag tatctccttc tttgcatata tatacgatta
    15901 tgctaggagc tttagataaa tcatatacac cttggtcttc tcccttgata ataaaatttt
    15961 ctgctttctt atctaatgct tcagtaaatc tcttaatttt gataatcagg tctatctggt
    16021 ccctattcaa atcaacttcg actttatcaa tacatgctgt attaaacaca gatgctgtat
    16081 cagttagatt atccatttct atatcatgtt caaatggaat ttcctcacta attttataaa
    16141 ccaattttaa accttcaact gggacaaata aaatttcaac ttttatgatt ccttgtataa
    16201 tgcttttatt tccttcaata taactatttt ctatagacat ggttggacaa acgcttacaa
    16261 tatcttttat ttgtatatca tcattatcat ttcttatgcc ttctctaacc ataaatgttt
    16321 cttcactacg tcttaaagtc ttatttaatt gaataggttt gtggtcaaat tttattatct
    16381 tttgaggcga atatgcatct tgtaatactt ctcttgtaac ttcatctgta acttttacct
    16441 tacaacaagc catacaatct atttctaaaa gacctgtgtt gctttcacta ttttgcttga
    16501 aaatatgatt aaaatctgac atagacaaca atacttcttc agtcatacca tcactaactc
    16561 cagggacttc tacaaattga gtaaactcaa tacctactct atccaactct acaagttcac
    16621 cctcatatgt acaagctaac ggatttattt ctaatactcc accaataata actttattgt
    16681 cagtcactct actttctttt actttaacac atggatttaa acttattata gattgaattt
    16741 cctctgtgtt tattgttata gtatctctta tagaactttc tgctttttct attccaacta
    16801 tatcttgaaa acaaatttcc tttctgtgct tttgtatgcc ttccacttgc gccacatctt
    16861 ttacaatgtc taacctttgc ttttcaaaca aacttcccct tatattcata agagctccta
    16921 ctttaatctt tctctcattc attatagtac aatccatatg ctctacttct gaaaataaca
    16981 tgtattccat atcttgaata acattgtctt tttctattac ttcgtttata tctacttttc
    17041 cgtctacatt agaaatagta tttttatcat ctgcaatata gattacatta taattaaaac
    17101 tacctctgca aagtatcttc ccatctgcca tttcaatttt tttaagtgat atgtatcctt
    17161 ctgtttttac aatttcatat acatctaatt ttttgtctgg tacaactgcc tccgcttcaa
    17221 tgaatgtttg aaattttcca aagtctattc tattgtcaac tttaattaca tctttaatta
    17281 attccatagt ctacctcctt aagtattata aaaatatcac actatattta tatttaagga
    17341 gcaaaaaaaa aattacacct atttttaaat aggtataatt ttaacttatt tgtaatttat
    17401 cttgatctct aaaaacttgc aactttacat tagatgttaa taaatctgag taactataag
    17461 atactctagg atatccatta ctttcatcat ctaactttat aacaaacacg cttggataaa
    17521 ctttttctag tataccctct tttgtaataa tctgctttct tcctttatta gctttcaaca
    17581 atattttctt tcctatatgt ctctctaagc ttactcttat cttatctaga gtttgaacag
    17641 tagccacaat atcaccttct ttaacgttta tatgtttaca taatatcaca tataaacatt
    17701 tttgtcaaat gtttaaacta attattttat atattttttg caaaaatgtc aatactattt
    17761 ttgaagatat acagtataat tatgttaatt tttgctaatg taatattgtt tttggcattg
    17821 cccatctaaa ttttcctctt gtttcagaac caccaattcc cttaacacct gtaatagtct
    17881 gctctataac ctcatcaata ggaactacta catcaattct tgaacaagca attttctcca
    17941 ctataaaaac agggtctaat gcatgtatca ttattcttcc aggcgaactt gcatagtttg
    18001 ctcctgcact tattattttt tcataatttg attggcatgc tcctgcgaat atgacaagat
    18061 tatccaaatt tggttgccat ttacgtgctt cttttactgt tttaacaaaa tttgaagagt
    18121 ttctatagtt actcatatct tctatatctc ctctttttat tgtcattgca tcatgacctg
    18181 ttattactag tatatccgga ttatattttt ctagtagtgc tcttacttct ttatactgat
    18241 tttgctctga aacagctaca cctactgcag gtattccaag ttgagcatat acatccaagc
    18301 aaatttttaa atattcttta tctccatcta tctgtagtac ttttccaggc attccatatg
    18361 tgttaggatt taattgaagt tttggaactg ctctagtcat tcttttttgt ctttctttag
    18421 cttttcttac tgacttatat aataaatttt ctacattttt atctattaat atatctttta
    18481 tatcaggtgc ttttactaat tctaggtcat caatataagc atctgctatt actctaaatg
    18541 caattccctt tagtatagct attttctcat tattttcatc tacaccaaaa gagactattt
    18601 taaatactat atctttgtta tgtgactttc tagctacaat gtctcctacc ttcatagtta
    18661 aacctccaat aaatttttat cttagtttat actatgttcg atataaaact ttgtttccta
    18721 atgtcaaaaa aacatttctt ataatacttc ttttatcact cttaaagcat ttttatagta
    18781 tatcttttct atatcatctt cactaaaccc ttcttttttt agaggttcat acagtttacc
    18841 catctgagat atatcttcaa tctcaacttt tgaatctatt ccatcaaaat ctgaccctag
    18901 agatacaaca tctattcctc caacatttac aatatgtttt atgtgtctaa ccatatcttc
    18961 aagtttactt tcacttttat cacttaaaaa taagtggaag aagtttatac ctgtaactcc
    19021 acctttattt gctaatactt taatcatatc atcagttaaa tttctagaat gattcatcat
    19081 tgctcttgaa ttagaatgtg tagcaattat tggtttagaa gaaatctttg caatttcata
    19141 aaatccacca tctgaaatat gagaaacatc aaccaacatt cccaattcat tcatcttatg
    19201 taccacttct cttccaaaat cagtaagccc tttttctcta tattctactt tattatgagg
    19261 aaagctcaat tcattcacat gattccaact tattgtcatc attctaaccc ctaagtcgta
    19321 aaatttcttt aaattttcta atttaccttc aagtactgca ccttcctcaa tagataaaag
    19381 agctgtaagt ttaccttctg attggttttt cataatttct ttatagttag tagctaaagc
    19441 tatttcatca ctattttttt tcatttcctc atgaaattta tttgccatac tcatgcaata
    19501 atcaaaagga tgttttactt cttcagtatc aacaaaaaga gcaaatgttt gagctaatga
    19561 atcccccttt ttcaatctgt ctatatcaac agaatattta ttagacttaa gttcacttgt
    19621 ttctacattt tccattaatt ttgctatagt atcacaatgt aaatcaagaa atttcatatt
    19681 tataccccct gtatatttta tatttatatc taaaaataga aatagttgtc cttatagaca
    19741 actattgaca actatttttt aatattcttt ttaatattac ttaatcttat tcttctttta
    19801 agttaggtat tactttttta acttcttcaa tttgatgcca tgttttatct atattatttt
    19861 cactaatata tttttttaaa tcagttaaag atgtaaaatc tttaactgat aaattgtcat
    19921 catcatctgc aaatcttaaa ataaacatga gactccctta ctttccacaa catttcttat
    19981 atttttttcc gcttccacat gggcatgaat catttcttcc tatttttgct tctttaacta
    20041 ttgttttaga cttcttataa gcctttatta tttcttttct ttggtctgct ggtagtatat
    20101 cttcccaacc ttgtaaatta tatagccaat gagcctcaac agttaccata ttgttatata
    20161 atttttccca atctattttt atagaaactt ctttatcagc ttctacttct tctaaaacta
    20221 tatcttcttt taagctttca cttattccat ctatgaatcc catgaaaaat tcattagtag
    20281 ttccaaattt ttcagccaat tcacttactt taccttcaac aacatccatt ttattattta
    20341 atatttcatt gtatattcca gcttcaactt tcaaatattc ttcccagaat tttacttctg
    20401 cttcttgact ttcatgatta tcacttaaat ttctccattc agtatataaa ctcatacttt
    20461 ttcctcctaa gtatacctat atttttatag tttttatagt ataaaatctt ttagttagat
    20521 tggaagtttc ttctttttga ttataccata ttctatttaa agttaactct tatattatat
    20581 cataattaaa caattctttt aatactttta tagcatactc ttggtttata aaataagggc
    20641 ttgtcgcaat tgttattact ttggcttttt taattaagtt ttttatcttg taaaatttga
    20701 gttcaaatgg tatatagtcc atatcacttg aaaatatatc caaatctatg tcaagaacaa
    20761 actctgattc tacttctaaa tcaaatccat atagactatc tattattatt aattctgaaa
    20821 atatgttata ttttaaagca ggttttataa aacttcccac atttaaaact tcatttgtat
    20881 atctaaatac atcttttaga ttatttacat ctacatcata gttctcaggt tctctagtat
    20941 ccttatgttg gtctacatgc actaacttgc atccttttgt aaactcaccc tttaacaaag
    21001 attttgccca aaaataaaat gcatggttat gattgtcaaa cacatatatc tttttatttc
    21061 ccaggacata ttcgaccata ttctccaatc cttttgcttt tatttcctta ttttcatcta
    21121 cttcattaaa tactacctca ttgcctaacc ttacatcatt taatgtacca tttataagtt
    21181 ttggaacgta tatgctctta ttttctcttt tatcatatga aaatacatta tttcctactg
    21241 gtttttctat ataaaatcca ctatattcat aattttccat aaaactttat ttcctccatt
    21301 gttatataaa tatataagtt ataattttaa aaattaaaat gcacttattg taaatcatat
    21361 catctattat agaaaacatc ttatataact aaatataata tcttctctca atatgtatta
    21421 tacaataagt gcaaaatata tttatataaa atatactaca ttcctataac aaaaccatca
    21481 gcttcaattg gaaggtaact aactcttcta ttcaactcca cctttccaaa aaatactacc
    21541 caatatggtc taaaaatact ttcaatttta actatttcaa tctctgggat tcctcccata
    21601 aacctatgag ttaatctcat agccatttta tttgcctttg cttttactct tcgttcgtcc
    21661 ttatcactat actgcacaat attacaatcc acatttttta caacttcata tgtaggaaaa
    21721 tcttgtacta tactaccaga acctgtggaa ccattcacta aaatttttaa ttcatttata
    21781 tcattagaat atctcctatt cttgaaaatg ggtttcgttt tgtgaatttt aagtgtgata
    21841 attttaaact ctatataatg aagtctaact tcatgagcat atttgttctt taatattagc
    21901 ttactaaata aagaattttt tccactttta gctatctcta tagcttcttc ttttgtaatt
    21961 cttataggta acgctcttat atccataaaa tcattctctt tcttattttt ttcttgtcat
    22021 ttttaaaact ctatatatat aatcaaaaaa tattacaatt acaactgcca taactccata
    22081 tggaattatt tttccaacta taatatcatt tctagtcaat ataccataaa atataaatcc
    22141 tgttattaaa aagtacaata atactagaaa accgtctatt ccaagccatt ttatttctaa
    22201 atcttttttt accttagaca ctaatttaga catagcagtt tctcctttta tagattattt
    22261 tgtgtattta aattttcacc tattctatta ccaatcatcc atgcaataaa tgaaaaaggt
    22321 atagctatca caagaggctc taactcaaag ctctgaactg gaaatatttt aaaataacca
    22381 aaagtcactc caccaacaat cattcctaaa ataccaccca tagatgttct tcttccattt
    22441 ccaagaagcc cacctactat aggtacaaat gccgaaccca tccaaatact tcctacaaat
    22501 atacatgcat catatacatt ttcaaatcta aatgctacgc taagccccat aactgctgct
    22561 aaacatacac ctattctagt aaactttact aattgtttct gagataactc tttttttgta
    22621 attcttccat atatatcatt tgctaatgta gctccagctg ctagataata gctatccaat
    22681 gttgatatta tagaacctaa aattcctatt ataaatatta ctcttattcc ttgtggtaat
    22741 gtatctaaaa ctaaagttat atatccagca gctggagcca tttctggata agtagctctt
    22801 actataagtc ctgtcatagt taacaccatg tcaaaacata tccatagtga aaaacaagtt
    22861 aataaggctc ttcttccaac cttaggagaa tctgctgctg aaaatctttg ataaaaagtt
    22921 gggtctgaat aagctccaaa agaatataca attaacatca atgctttcat agctgacata
    22981 ccttttgtag ggtgcataag ctgtggtgca tctttgagtg cttctgtaag accttgaaac
    23041 cctcctattt gttcaaattg acctggcata accatagtta aaccaaatac catacaaaaa
    23101 aacaatacca tatctgtaat ggcaactccc ataagcccac caagtactgt aagtacaacg
    23161 actactccta ccattattaa tcctattaat tctttatcta ttttccatgc tacgttacct
    23221 atagttccca tagcagttat ttctccaagt tgtgcacaat atataaacat caaaattgcc
    23281 ccaattatag caacatgctt gccataaaaa ctttccagta aatcaggaac tgttatattg
    23341 gttcttatat gaatccttgg accaacccac aatgctaaag gcattctccc aatatgtgca
    23401 gctattgacc aaactgccca tgcagatatt ccatatatag ccgcatactc tacactacct
    23461 actgtaccaa ctcctccata ccatgatgca accaaagttc ctactattaa ggaccatggt
    23521 aaacttcgat ttcctaggta ataactttcc atatctttta catttctaga aaaataataa
    23581 cctattccta ataatataaa tgcaaatata actataatcc cattttccca aaatgttaac
    23641 atatctaatc tccacattct tatattttaa tttttaactt taatatctaa atatgaacaa
    23701 ctctacccat ttccataaaa ataggtagag ttatataata attataagta tttaagtaat
    23761 cttgttatta agcaatttta ttgtttaaac ctttattttc tcttttattt attttattta
    23821 aagcataagt agcaaatatc cctataactg cacatactac agataaaata aatatatatg
    23881 tatatcctgt ttgtccatat ttatctaacc aattacctgc aatagtgtaa taaaacgcat
    23941 ctggagaaaa tcctataaat gaagcaaatc caactactgc tcctgtagtt tctaaagata
    24001 tattagactc tgaaacagat gcaaagtata tacctctaaa tccaaataaa attacactta
    24061 gtattatcat gtttattaca gctatccata taaatcctgt actcttagga ataaccaagt
    24121 aagcagctgt acttactgtc attaatataa atcctacaaa cattactctt atagatgaac
    24181 caactttatc tgttataaca ccagcaactg gagatgctcc catttttata acataagttc
    24241 ttattattga taaagcagaa actagagtca ttggcattac ataaacttct gataagtatg
    24301 gacttaaata agttaaactt gagaatacta aataagttga aaatattata agaccaatta
    24361 accaagcctt tggcattgta actactttta acaatgactt aatacttaat ccttcatctg
    24421 atgctccact atcttttttc tcttctacta agaaaaatag aagtatacca catattatag
    24481 atgctgcaga atataaccat actacatatt tgaatcctac tgttatttca gcaaatttac
    24541 caaagaaata aagacctata aaagaaatta atgtacctga tacacctgat aacccttcat
    24601 aaaaaccaaa tattcttcct tgctctgact cacttccttg cattctaaca accttaacag
    24661 aggctgagaa gaaggttaat attgtcgtaa atccccataa tacaaatatt aaaagtatca
    24721 tactatatga tgggaatgta gagaaataaa atccaagtat tcctgaagca attaaagaaa
    24781 aagttattaa ttttctaact gagaatctat ctgcaaacca tcctcctata aaataagata
    24841 ccattcctac taatccatat aaactcaata agttccctat ttgtgtatga cttaatgcta
    24901 aagcttgttg cattgggtca taaaatgatg atttcatata tggtaaacta tacatagctg
    24961 ttgttccaaa tgccaatatg aatattatca aaaatctttt aacattattt ttgctttcca
    25021 ttgattctct ctccttaaaa ttttattgtt tataatctca ttcatgtaat tttattttaa
    25081 tttttttatt gcttgtttaa agtcatttat tatatcattt gaagattcta atcctacagc
    25141 tattcttatt agattttccc cattcatttg aactaatgta tgctcttccc ctaagcttgt
    25201 tgctattgtt gcaagctcta aagaattcat taatcttcta ctagcttggt ctcttgtcaa
    25261 tccattgata ccatccttaa gtttaaatga aactacacct ccacctaaac cattcatttg
    25321 agttttacaa agctcatatt gaggatgtga ttttaatgct ggatacttta ccaattccac
    25381 attctcttct tcctctaaaa attctgctac tgctaatcca ttttcacaat gctttttcat
    25441 cctcatatct aacgttttca taccccttaa aatcatccat gcactcattg gtggtaaaga
    25501 tgctcctgta aaacaacata aacttggcca tctaactaaa tctattaatt cttttgaacc
    25561 tgctactgct ccccctaaag tatctccatg tccatttata aacttagtaa gactatgtat
    25621 tactaaatct gcacctaaat ctattggtct ttgtacaatt ggtgtcccaa aagtactatc
    25681 aactatagct aagcttttat tttcatgagc aatttctgct attgttttta tatctactaa
    25741 gtctaatgat ggatttaaag gactttctat ataaactatt ttagtatttg gtctcattgc
    25801 ttcttttaca ttttgtggat ttactgcatc tacaaatgta acttcaactc caaatttaga
    25861 taacaactca gacatgaata atttagtgtg tgaaaatatt ccatctgaac taactatatg
    25921 gtcaccactt tttaccaatg ctaatatagt agatgttatt gcccccatcc ctgatgtact
    25981 agatatggca ctttctgcat tttcaagcat agccaactta gcttctaact ctgtaagagt
    26041 aggattacac tctcttgtat acacatatcc ccatgtcata cacaactctt caaaatgctc
    26101 tactgtcttt gcagcaaatg ttgctgtttg acatattggt gtagccaatg cacctgttgt
    26161 tgggtctgca ctatgtcccc aatgtattat ttttgtctct aaatcttgat taatattgtt
    26221 actcattttg atttcctccc attttgactt atttatacaa cgttttccct accaaatttc
    26281 atcaatttag cttagtctta atacattttt tcaagtatac ttttttattt gttataattt
    26341 ctaaaaatat gacacttatt ttgttttttt acagttctaa ttataaactc tatagtaact
    26401 atagagtcaa tatgtttttg atattttttt tacaatgttt aaaatagtca gtacaaaaat
    26461 aggtatatct tagaataact aaagtcatcc taagatatac ctatttttat atcacaatat
    26521 aaataattac atataagcat tagttttttt atcataaata gatttttcaa gatttatcat
    26581 cttttcataa atatattata gttaaatatt ttaatattac ctaacatcta caataaaata
    26641 tactcttaaa ataaaattat ataattaaaa atactctata aaagctaaaa agtttttcta
    26701 ttcgctacat ctaaaaataa ttagatagag gtataaatgt atttagttta tttatacctc
    26761 tagtctttta taaattatat ctctactaaa ttgatttttt agcacgaata agtgtgctct
    26821 tggagatacc agtcatcttc acaacctctc tatatggctt agaatttaat aaccccaatg
    26881 catattgtat ctgttgattg ctgtatttct ttggcctacc atctttaaag tcctttttct
    26941 ttttagcaaa ttcttttcct aattgagtac gttctattat catatttctc tcaagttctg
    27001 caactgcaag taatgtagtc aaaaagaatt tacccattga agtattttct aaaaggccta
    27061 tatttaaaat atgaacactt acttctttag caaataaacc ttctattagt tgtattcctt
    27121 ctgatgtact tctagctagt ctatccagct tagttgcaac aagtaagtct ccctttttta
    27181 gagatgatat aattttttca aatttaggcc tgtttgtagt agtgcctgta taagtttcct
    27241 ttattatttt agcatcatta tatttattca aaatttcttg ctgttgctga tacaaactgt
    27301 tgttgtctgt ttgatttttt gtacttacac ggcaatatcc atatataata atcatcactt
    27361 cctaacataa agattttgta cagaagttac ccagaagctt gtttttactt atctttatcc
    27421 attttactaa aacgattttt gggtgtcaat actgttaact tttgacacca taataattcc
    27481 tttataattt gtttgactat gtcaaaaatt accaatataa tgaaattaat gagtttatac
    27541 aaattggagg aaataatatg aatagtctgt taaaagaagc aatagatgaa ttaaaaaaca
    27601 tccattgcgg ggaagttttt ttattaaaag aattgttcaa aggctatgaa tggaacaggt
    27661 tggagatgaa tatcagattg aaattaggaa aacttttttt gtatgaagtg gtaactaatc
    27721 ccaatttaaa tgtagaggtc atcaataaaa attctgctta tcagcaaatg tataaaaaaa
    27781 aatagtgact taaatgtata taatagtata atcttcttat tgattcaaaa gttaaaactt
    27841 ttaatacaaa acaatcattc tattttaaca tttatcaata tgtctaaatt tatataatta
    27901 acacatacga atagtattgt ttaaatcaca ttatttaaga tagataaatc ttaaccctac
    27961 tccagcataa atactaaaat agggcttaga attatctata tttttatccc ataaaatgct
    28021 tagctcttcc tcttgattga acatatatat ctggggaaat acaatagaag cgtaaattta
    28081 taaaatttcc aaagtcagaa aacttcagtt caaacttagg tataccaaaa ctttcaggtg
    28141 ctatttcgtc aacgccaaaa tagatgataa ttccattatc tgtaagataa aatgcttggt
    28201 cttcaggtat aactaactca acattagaat aatacatttc tctattttca tttatttgag
    28261 atttaatgta atcagtaatt atattaatat aatcaactcc atctctaaaa atgtcattaa
    28321 gtaaaagttc ttttccagtt gtcaaatcta tattgtaatt attcaatgta ttgtatttag
    28381 gcccatagtt attagcaaac cccataaggt ttaatatcat actcagtaca taatttttat
    28441 taaaagtaac tgcgtaactg gtagataccc aatagctttg ttttggaagt gaattttgag
    28501 aagctgcgtt attatattct cgttcttctt cctgtaagcc atttttaaaa ttatttacag
    28561 tcattcttac agcattgttt atgttattta aaataactgg gtttacatac gtggattgat
    28621 ttctaatatt gtatataaca ggatacttta catcatatga aaaatactta cttctttcaa
    28681 aatcttcatt aaattgtaca tataatatat tatatgtaca ggtatttgta gattgataat
    28741 tttccattaa aatcctcccc cttttataaa aaaatcagat acaatataat atgcttaaaa
    28801 cagtaataat gttaatggta aatgtaaaga atagaaagta tctaaaacag caaaaaatga
    28861 ctaattttgt ggtataatac ataggattga gagttatttt ataggaggaa aaatatgtta
    28921 ttattagcag ataaatggaa agattatgaa cttatagata tgggaaatgg agaaaaatta
    28981 gaacgttggg gaagttatgt acttagaaga ccagacccac aggttgtatg gccaatggaa
    29041 tctgaatggg ctttatggaa aaaccctcat ggacattatc acagaagcaa taaaggtgga
    29101 ggtcaatggg aacacaaaaa aagatatcca gaacaatgga ctataaatta taaaaactta
    29161 aagtttcaca ttaaaccaac tggctttaaa catactggat tattcccaga gcaagcagct
    29221 aactgggatt ggatgataga gaaaataaag aaagctaaca gacctataaa agtgttgaat
    29281 ctttttgcat atacaggtgg agctactgta gcatgtgcat ctgctggtgc agaagtatgt
    29341 catgttgatg ctgcaaaagg tatggttaat tgggcaaaag aaaatataga attatcaggt
    29401 cttaaaaatc aaacagtaag atttatagtg gatgatgttg taaagtttgt tgaaagagaa
    29461 ataagaagag gtaaaaaata tgatgcaata ataatggacc ctccttctta tggaagaggt
    29521 cctaaaggag aagtatggca aatagaagaa aagttataca gttttgtaga tttatgtatg
    29581 ggagttttat cagataaccc tttatttttc ttaattaatt cttatacaac aggattttct
    29641 ccaatagttc ttgaaaatgt attgtctaca acaataggta aaaagacaaa tacaggagat
    29701 gtatacggtg gggaattagg aataccagct tccagagatg gtaagatact tccttgtggt
    29761 atatttggaa gatgggagaa aaaataatga taaaagtcat atatgaagat aaccatttat
    29821 tagttgtaga aaaaccagtt aacatattgt ctcaaggaga taatactaat gataaagata
    29881 tggtaaatct tttaaaagac tatattaaaa aaaagtataa caaacctgga aatgtatata
    29941 ttggtttaat acatagattg gatagaccag taggtggagt tatggtgttt gcaaaaacat
    30001 caaaagcagc atctagattg tctgagcagg taagaagtaa gactttcaaa aaaacttatc
    30061 tagctgttgt tcatggtaag ataaaatcta actcagatat actaaaagat tttttatata
    30121 aaaataaaaa aactaatatg gtaactgtag tgaaaaaaag ccataaagat gccaaagaag
    30181 ctgaacttaa ctataatgta ttagatttta aagaaaatct aagtttagtt caagttaatt
    30241 taaagactgg tcgttctcat caaataagag ttcaatttgc aagcagaaaa catccattat
    30301 acggagacca aagatatggc aaagaaataa ataagattgg acaacaaata tctctttggt
    30361 ccaataagat agaaatttgc catcctacta caaaagaaaa aatggaattt acatgtcatc
    30421 cacctgacaa atatccatgg aatatatttt aaatctcaaa tgaggtgatt atatgagtaa
    30481 aaaattttca ataggagaaa tgtctaaact tcataaaata agcatacaga cactaaggta
    30541 ctatgaccaa atagatttat ttaaacctgt tcatgttgac caagaaagca attacagata
    30601 ttactcaatt gaccaatttt cacaattaga tattataaaa tatttaaaat atttaggtat
    30661 gcctttaaaa gaaattaagt caaagcttga tgaaaataat gaagaaattc taaaattact
    30721 aacaaataaa attaatatag tggatgaaaa aattaaagaa ttagagctta taaagaaggt
    30781 actaagcaca aagaaagaaa ctatggaaaa aggtatgaaa gtagatgaag ttggtaaaat
    30841 agtgagaaaa catattccac gaagaagtat cttatctata gagtatgaag aaggattaag
    30901 tagtgaagaa actatggaac tttccagaag acaaatagca aatatattag aagataatat
    30961 atctgtgttc tatggtggag taagtggact tgtatctata gataaatttt taaaagaaga
    31021 taaagttatt tatgacaata gttttgttac tgttgaaaga gacttatttg atataaaatc
    31081 acaaaaacat ataacagaaa tttcatctgg tgattttatt tgtatgacat ataaagggcc
    31141 ttacaaagaa aattataccg cttttaagaa actggttgat tatgttgaat ataaaagaat
    31201 acctattgaa aattatatat atgaaatacc tataatagac cctctgtcta caaacaacga
    31261 agaggaactt ctaacagaaa ttcaaataca agttaaaaag agagaaattg acttaagatg
    31321 tctagtgtag taatctagtt tacttacaca tcttaatata gttgtctatt atcaacatat
    31381 gatttttcta gatagtgatt cctaacagtg agattttcaa aaaataggag gaaatatggc
    31441 taatgctaat tccctcctat ttttaattaa gccatatcat tttaattaag ccatgtcatc
    31501 acgaagtgca tcaataatat tttctttttt tatcttatta atagtataca acattgtaat
    31561 aaatactata aataaaacac taaatatact aattccaata ctaaaccatg gaatcacaaa
    31621 atcaatatca tctgtaacca ttactttatg aattagccat gaagaaatta ctgctatagg
    31681 aagtccaaag aacaatgctc tcatgccata aaaggcacac tcaaaattca tcatcttttg
    31741 gaaatcatgc tcagacattc ctacagagcg aagcatggca agttctctac gacgtaactt
    31801 aatattagtt gaaattgtat taaacacatt ggcaatcgaa atcagagaaa tcataattat
    31861 gaaagtataa gcaaaaacat tggcaataaa aatgtaatta cggttctcat ccatcattcc
    31921 atacatattg tacagattgt actcattcgt aactccctca ctctcaataa tttgctccat
    31981 ttctgtaact gactgagtag gatttttcga ttgaaagctt aaactttcaa ttcctgtaga
    32041 aacaccagga atctcaaact tatctttggc tgaataagga gccaatactc tgaagcttgc
    32101 tttcttcaca tctgaacttc tgattcctgg aggagaatca ggtggcacta ctttgacaaa
    32161 tgaaatattc acattttgtc tctcaggtat ctccaacttg ccattagttt caggagtgag
    32221 gctaacattc atagaagaac tgttgaaaat atcaataata ttattatcct ttgttaccat
    32281 agaaacagca atcagttttg cattttgtcc agtatattct tctactggta atccaagact
    32341 tttaataata ttcagataag ttctatcatc aagaaattgc atatctactg gtaattcaag
    32401 catttcactc atagaatgtg aaccctcata gttcaaataa tcgtctgtaa gctcacttac
    32461 tttggcaaca catgaatagg tcataagttt ctgatacaaa ccttcgtaaa caccatttgc
    32521 agtttttagc ttatcgtaaa gcttcaacat ttcgttgtct ttcatacctt gtgtagaaaa
    32581 aataatatcc tggtcagtaa cctctgacct ctgatttgac atctgtttta ggtctgctac
    32641 taaagcactt gtagatataa ataaaactat gcttaaaaca agcgacaata caatacttcg
    32701 ataacgtttc ttgtttcttt taaagttttt catagcaaga actccctcaa gaccataaac
    32761 tcgctgtgtt agttttgatg tcttcacaga tttagattca attttcacct catttgtctg
    32821 acgaatacac tccatcactg gtgtgttggc agcctttctt gctggtatgt aagcagaaat
    32881 taagattgta accatactta ccactgctgc aacgatgatt gcagggatag acaatgttaa
    32941 tgtcaacgga acgtttacat agagaatatt tgcaaagttt ttagctatga cagaaatcac
    33001 aagtccaata ctgactatac cgattgcaac accaattggt ataccaatcg caccaatgca
    33061 aattccttca aatagcaccg aatttcgtaa ctgttttgct gtagctccaa cagatgaaag
    33121 tattccaaat tgatgtgttc gctcgttcaa tgacatgtta aatgaattat atattagaaa
    33181 aattgagcct agcatgatta tggcaagcac aatgccacca accgagtaca aaaacatatt
    33241 gaacaaattg ttatcagaaa cacctatgaa gcgcaacaca ttgtcattga aaatggaagc
    33301 atggtcttta gtgctactta tgtaagagcg aatctgatgt ggatttttca atgtgacaaa
    33361 aaggctaaga ctgtcagtct tgacttgttt atcttctttg gttatcaagg tatatcctgg
    33421 tgcagaagat tcttcaaata cgggctttct acagataccc acaactgtat aagttttctt
    33481 atcctgtggc acaaaaactt cactcccagc agtgtatggg tcactctgac caagcttttc
    33541 attttcactc attcgtttcc ctacatccaa agaaatagta tcacctactg caaatttgac
    33601 accaccatct gtcgcaactt tacttgaaac aagaatctct ctactatttt caggtaatct
    33661 tccagaaatt aaagttatag gcaaagtatc aaaagcttct ttgctaaatc cagcaataaa
    33721 aagatatggt ttatctggat ttctgttacc ttcaagtgtt gcatagccaa tattttcaaa
    33781 tgttgcagta tttgcaactt catcattgac tctttgttcc tgtacaaaag aagaatccac
    33841 atccaaaaac tggacatgcc aatcaccata ttttataata gctccatttg tcatatagtt
    33901 catcaaagaa actccaaagg tagtaactgc tgtaatcata gcagctgaca gaacaactcc
    33961 tataattgtt acaagagttc gagtgcgatt ctttttcaat ccttgcagag caactttgtt
    34021 aaaaatgttc attaacgatt caccttcctc tcatcacgca ctacttttcc atctgaaata
    34081 cagataattc tatctgcctg taaagcaatg ttttcatcat gtgtgacaat aatgagagtt
    34141 tggtgatatt tactatggct ctctttcagt aactggataa tctcttgtcc atttttactg
    34201 tctaagctac ctgtaggctc atcagcaagc ataactgctg gtgcattcat caatgcacga
    34261 ccaatggcaa cacgttgctg ttggccacca gaaagttgat ttggtaaatg tgtttttcgt
    34321 tcttttaaac caagtaattc taacaagtca tttaaccgtt cttgattgac cttacgctta
    34381 tccataagta taggcaatgt gatattttcc actacattta atgtgggtat cagattgtga
    34441 aactgataaa tcaatccaac ttgtcgccta cgaaaaatag caagtttttc attgctttgg
    34501 ccatatacat cttgcccctc cagatatatc tttccacttg ttggtacgtc tactcctgca
    34561 atggaatgta gtagtgtaga tttaccagaa ccagaagaac caatgattgc agtaaactcc
    34621 cctttttcaa tagtaagtga gacatggtca agtgcagtaa ccttattttc attcttacca
    34681 taaaccttac ataaatcttc aatctttaaa aacgccatta tataagtctc ctttcatatt
    34741 ttataaatat atcataggac tttcaactta catttaagtg acatttaaat gagagattcg
    34801 tcactttgga aaacgaataa caaatattgc accaccttgt gggtgatttt gtgcagtaat
    34861 tgttcctcct tgtctcataa taatcgtctt acaaagtgcc aatcctattc cataaccggc
    34921 tgtgcttgaa tttttcccac gataaaatct gttaaataga catggtaagt cttctttttt
    34981 aaagcctgct ccgttgtcat gaatagtaag ttgagtaaac aaaaagttgt cctcacaaat
    35041 gatatcaatt tttccattat cacccacact ctccatgcag tttttaaata tattttgaat
    35101 tgcttctgaa agccaattta aatcacccaa aatccttact ccttttggta tatcaatatg
    35161 taaagtgata ttgtgcagtt ccatgggaat caaaagttga tgaagtgcag aactaatcaa
    35221 attattcaca tcaatttgtt ctttttgaaa gactacaata cctgcatcta aacgtgataa
    35281 ttttaaaagg gaagtaatca gccagtccat tcgtataagt aattcctctg tttctcgtat
    35341 caactctttt ctctcatctt catcagaagt attctctaac agtgacaaaa taagagttac
    35401 tgatgtaaga ggagtgcgaa gctggtgggc tatgtcagct agtgagtcag caaggtgttc
    35461 tttttctttt ttcagtgcgt aattttgctc tctaatgcgt aacgtcattt tagttatttc
    35521 actttgtaga atagaaagct caccctcatc tgattcagca atatacagat ggttagcatt
    35581 atgaagcaca agattaattt gttctgaaat ctgtgcaata cttttgtatc gatttttagt
    35641 aaacacaaaa aatgctattc caaaggtagt ggcagaaaca atagaaagta ttccagtcat
    35701 aatactgatt gcaaatccca gggctacagt gagaatagta attaataaga ataaaatagc
    35761 aaattttcga aactctctat ttcgaaacat aacttccccc caatctatac cctgtcccac
    35821 gaacagtcaa aattatttgt ggacttgaag ggtcatcttc tatcttctct cgcaagcgtt
    35881 tgatgtacac agtcaatgtg ttgtcattaa caaactcacc tgccgcatcc cacaattcat
    35941 caagtaatct taccctcgta ataatacttt taggattgct aataaacacc aataacaaac
    36001 ggtattctaa agctgaaaga aaaacctcat tgccattctt tttcacaaca ccggttgccg
    36061 tatctacata aagcccacaa atttcaaaag ttgacccaaa acgcccactt tttcgtagtg
    36121 ctgttctaat tcgcgcaatc agttcacgtg gacgaaaagg tttagtaata taatcatctg
    36181 cacccatatt cagtccagta acaacacttg attcatcacc ggaagctgtc aaaaagataa
    36241 caggaatgtc ttgcttttct ttgatttctg tgcaaatcgt aaacccattt ccatcaggta
    36301 aagaaatatc aataagtgcc aaatcaaatt tattcttagc aatctctaca agtgcttcac
    36361 ttcttgtgga tacatgggtt actataaatc cttctgaatt aagcaaaaga ataagatttt
    36421 tggcaattgc tttatcatcc tcaactaaaa atatccgttt catttacttt catcctcctt
    36481 tattttatta gttttatata ttccatcaga gcaatagtac actactattt tacttatcat
    36541 tacacaattt tcagtagttt cttttgttct tatataatcc atgcataata cctccattga
    36601 tttattatat ttcttataac ataaatacac aagataagac aaaaaacata atgtaagtgc
    36661 aagtttttta actcactaac ttataaacat acactcctac taacactact ttcttgatga
    36721 cacatcttac aagcttatat tcatacatca atgaaaaatt aaatttatcc atttattaaa
    36781 ataaaaggca atagcttcta tagctaatgc cctttatctt aaaatgattt aaatactata
    36841 caatttttat aaatggctat aaataaactc gtatagaata taaaatttat ctacagtctt
    36901 ttgttataca aaatttataa cctcttatag aacaaattta ccactttgca aacttatcac
    36961 ttttactcag ctatattata aacttttaat ttatctttgt ttaaatgttt cttcttaact
    37021 tctactaaag cttttagcgt atccaaagat gtcattattt cagtagaaaa ttcaatggct
    37081 gttcttctca tcttaaatcc atctcttgta gagtcatttc ctttagttgg tgtatttatt
    37141 actatatcaa cttgtttatt tcttatagca tctaatatgt ttggtctagg ttcttcaatt
    37201 ctatttacta tatcagcctc tattccattt tctcttaaac tcttcgctgt tccttctgta
    37261 gcaacaaaag tatatcctaa ttctttcata tcttttgcta tctctataaa ctcatcttta
    37321 tcatgattat ttacagtagc aagtactact cctctttcat cagacatatg tctacctgct
    37381 gccaagaacc ctttatataa ggcttcttct aaattttctc ctacacctag
//
LOCUS       DAEMHZ010000025        36456 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000025 DAEMHZ010000000
VERSION     DAEMHZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.025"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            235..612
                     /locus_tag="KPK72_001163"
     CDS             235..612
                     /locus_tag="KPK72_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2488399.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            605..1753
                     /locus_tag="KPK72_001164"
     CDS             605..1753
                     /locus_tag="KPK72_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG2488400.1"
                     /translation="MNNYMLYVFENLIKTIIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNTNEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1770..2372
                     /locus_tag="KPK72_001165"
     CDS             1770..2372
                     /locus_tag="KPK72_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG2488401.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2465..3487)
                     /locus_tag="KPK72_001166"
     CDS             complement(2465..3487)
                     /locus_tag="KPK72_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2488402.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3816..3998)
                     /locus_tag="KPK72_001167"
     CDS             complement(3816..3998)
                     /locus_tag="KPK72_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488403.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4311..4730
                     /locus_tag="KPK72_001168"
     CDS             4311..4730
                     /locus_tag="KPK72_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG2488404.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIIQIENCIV"
     gene            4861..6702
                     /locus_tag="KPK72_001169"
     CDS             4861..6702
                     /locus_tag="KPK72_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2488405.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6729..8615
                     /locus_tag="KPK72_001170"
     CDS             6729..8615
                     /locus_tag="KPK72_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2488406.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDDSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            8623..10623
                     /locus_tag="KPK72_001171"
     CDS             8623..10623
                     /locus_tag="KPK72_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2488407.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10885..12144
                     /locus_tag="KPK72_001172"
     CDS             10885..12144
                     /locus_tag="KPK72_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2488408.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12205..13992
                     /gene="iorA"
                     /locus_tag="KPK72_001173"
     CDS             12205..13992
                     /gene="iorA"
                     /locus_tag="KPK72_001173"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG2488409.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13994..14569
                     /locus_tag="KPK72_001174"
     CDS             13994..14569
                     /locus_tag="KPK72_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG2488410.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14622..15692
                     /gene="buk"
                     /locus_tag="KPK72_001175"
     CDS             14622..15692
                     /gene="buk"
                     /locus_tag="KPK72_001175"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2488411.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15910..16611
                     /locus_tag="KPK72_001176"
     CDS             15910..16611
                     /locus_tag="KPK72_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488412.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16671..17175
                     /locus_tag="KPK72_001177"
                     /pseudo
     CDS             16671..17175
                     /locus_tag="KPK72_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            17350..18372
                     /locus_tag="KPK72_001178"
     CDS             17350..18372
                     /locus_tag="KPK72_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2488413.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIGALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18519..18827)
                     /locus_tag="KPK72_001179"
     CDS             complement(18519..18827)
                     /locus_tag="KPK72_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2488414.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18887..19315)
                     /locus_tag="KPK72_001180"
     CDS             complement(18887..19315)
                     /locus_tag="KPK72_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488415.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19538..20995)
                     /locus_tag="KPK72_001181"
     CDS             complement(19538..20995)
                     /locus_tag="KPK72_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2488416.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21413..22327
                     /gene="nadA"
                     /locus_tag="KPK72_001182"
     CDS             21413..22327
                     /gene="nadA"
                     /locus_tag="KPK72_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG2488417.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22340..23641
                     /locus_tag="KPK72_001183"
     CDS             22340..23641
                     /locus_tag="KPK72_001183"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG2488418.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23622..24458
                     /gene="nadC"
                     /locus_tag="KPK72_001184"
     CDS             23622..24458
                     /gene="nadC"
                     /locus_tag="KPK72_001184"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG2488419.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(24592..25413)
                     /gene="yidA"
                     /locus_tag="KPK72_001185"
     CDS             complement(24592..25413)
                     /gene="yidA"
                     /locus_tag="KPK72_001185"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG2488420.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMNLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            25906..27600
                     /locus_tag="KPK72_001186"
     CDS             25906..27600
                     /locus_tag="KPK72_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG2488421.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      27932..28042
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28146..28517
                     /locus_tag="KPK72_001187"
     CDS             28146..28517
                     /locus_tag="KPK72_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG2488422.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            28613..29560
                     /gene="trxB"
                     /locus_tag="KPK72_001188"
     CDS             28613..29560
                     /gene="trxB"
                     /locus_tag="KPK72_001188"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2488423.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKSEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            29664..29981
                     /locus_tag="KPK72_001189"
     CDS             29664..29981
                     /locus_tag="KPK72_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG2488424.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            30250..31536
                     /locus_tag="KPK72_001190"
     CDS             30250..31536
                     /locus_tag="KPK72_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2488425.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            31648..32121
                     /locus_tag="KPK72_001191"
     CDS             31648..32121
                     /locus_tag="KPK72_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:31780..31782,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG2488426.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            32153..33463
                     /gene="grdB"
                     /locus_tag="KPK72_001192"
     CDS             32153..33463
                     /gene="grdB"
                     /locus_tag="KPK72_001192"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:33200..33202,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2488427.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            33720..35252
                     /locus_tag="KPK72_001193"
     CDS             33720..35252
                     /locus_tag="KPK72_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG2488428.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            35263..36408
                     /locus_tag="KPK72_001194"
     CDS             35263..36408
                     /locus_tag="KPK72_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG2488429.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
BASE COUNT        14334 a         3947 c         6463 g        11712 t
ORIGIN      
        1 aggaattttt attaaatagt tttaatgtgt aaataacatt ctacactatt gtaattaaat
       61 atgtaaaaag taaaatattt aaatagatat attttaaaat ctaactttta atattgtaaa
      121 aaaattagaa atttaacaaa aagtacaaaa attcacattt ttttcataaa gtgtttgact
      181 aaaaaattca catgttgtag aattaaggta gcaggagttt tgagggaggt gtaaataatg
      241 ataagaaaaa taccacaagc agaactaaaa gtaatgaaat tcatttggaa agtagatgtt
      301 acagtgacat caaaagatgt tattgaagct atggaacaaa aatatggctg gaaacaaacc
      361 accacactga cacttttatc taggctggta aaaagaggat tcttagatgc tcaaaaaata
      421 gacagatata cacattacac aataagagta aaacacaaag attatttaag ttttgagaca
      481 aaggatttcc taagtaatat acatgatgac tctttaagaa gcttaatttc agcattacat
      541 gatgatgaaa atataggtaa agataagcta gattttctag aaagttattt tgataattta
      601 aaggatgaat aattatatgc tatatgtttt tgaaaatctt ataaagacaa ttattgtatg
      661 tagtcttggt atttgtctat tattattttt gaaaagatat ttatttaaga agtttagtaa
      721 gagattcaat tattatattt ggttgataat agtctttaga atgttacttt ttctatttaa
      781 ttatacaata gtatatgaag taaaagaacc caaagaaaat gcagtaggaa ataatattac
      841 acaaattgat atttctacag ataataatct tatgctgtat gtagcttatt tatggttatt
      901 tgtaactata gtcattgcag tttatacctt tataaagtat acaagattta aaaatctagt
      961 tgttgatgta tcttatgata ttgaagataa tgatataaac tgtatctaca aaaacttgtt
     1021 aaaagaatta aatataaaaa agaaaattga attaagaggt tctgatgaat tgataagtcc
     1081 agcaggcatg ggattattta aatcttacat attcttacca gactaccctt atagtaaaga
     1141 tgaactaact tggattttga aacatgaatt aatgcatttt aaaaataaag atattttaat
     1201 aaagttttta gttttgtctg ttagaataat atattggttt aatccacttg tatatgtaat
     1261 gagcaataaa gtaaatctag attgtgaatt atgttgtgat gaaagtgttt tggtagaatg
     1321 ttctttaaaa gataaaaaag aatatgcact agcacttata aagtcaatta agtttagtaa
     1381 aaattataat agtaagattt tgacaaccga gtttgataag acaaatttgg aaaagagact
     1441 tgagagtatt gttaaaaaga aaggcaaaag tggaatacta attgctgttt tagtatttat
     1501 gacttctata acatcttttg ttgaagttga tgctcaagtt agaattgatg agggtaataa
     1561 tggaatgaat aacaatacta atgaattttt attgaataag acgactgata aaattataaa
     1621 tatttattat aaagatgcac caaatgaaat taggaaattt tatgaagata aatgcgaatt
     1681 agaaggcaaa atacctagag actatgacat aatagaaatg gatatgaaag attataatca
     1741 attaataaaa taattataag gaggatttta tgttaacaaa attaagaaaa atagtagcta
     1801 ttatgtgttg cttattgctt acttttacaa gtgtggcatt tgctgacaca agtaaagaga
     1861 aaggaggaag taaagagact gttccagaga aaaacctaga ttcttttact ctttctgggt
     1921 atatatatac atatgaaaat gcgccacaag aaataagaga tgaacataaa caaaattgtg
     1981 aagagataaa tatagaccct aagccagatg atgagatatt tgttccagaa tcagctaatc
     2041 taatgaatga ggacaatagt aaaggtgttt atgcaagtta tactgtttct tataatatag
     2101 gtgctaaaac tataacaatt atgagtaatg agtatttgac tataagtaca acaaaagtta
     2161 tacgaaaggg taatagcgga aaagaagtaa aagctgcaca aatcatgtta actttacttg
     2221 gttataatgt aggtatagat agttcttttg gaagtaagac atataatgca gttgtaagtt
     2281 tccaaaaaaa atacggtttg tctgcagatg gaataattgg tccagctact tgggataaat
     2341 tgggtagact aactgaccct actctttcat aggattaaaa tctaaaaata tattaaatag
     2401 tgtctcaata tgatttttca atttaaatga gacactattt aaatataaat gttataaaac
     2461 tattttacaa taaattccat ataactctac catctatatc cttaacattt tgacaaccag
     2521 tcaaaatcat agtctcacat aactcattcc taaccttctc tataaaaaac tccacaccat
     2581 caagaccacc gccaaaagaa gcagtaacaa aaggtcttcc catcaaaaca gcatcagcac
     2641 ccaatccaag catcttaact acatcaacac cagtcctaac tccaccatca acaagtattg
     2701 ttatctttcc ctttacagac ttagctatat ctggaagtac ctcacaagta ccaggagtat
     2761 aatcaagtac tcttccacca tgatttgaaa caataatggc actagcacca gactctacag
     2821 ccatcaaagc atcatctaca gtcataattc cttttataat aaaaggtaat ctagtggatt
     2881 caaccaattc tctaatctca tcaattgttt taggagaaaa aggattttct tgaaattgat
     2941 tattaataag tccacaagca tcaaggtcta caccaactgc aaaagcacct gcttcttcag
     3001 acaatctaat tttatctatt atctttgaat tattccaagg ctttataaat acaattccat
     3061 ttcccctatt atccttcaat acatctaaat tatccagtaa aaaagtatct acattagtat
     3121 cgccaaccat agcataaatt ccagaattag aacaacctct aacaacaggc tctatgtatt
     3181 ctttttcgga aactttacca cccatgttca atatagtacc tgatacaggt gcagcgaata
     3241 taggagaact catttttcta ccaaataatt ctatggatgt atctggcttt gaaacattat
     3301 gtatgactct catatttatc tttatttttt ctagactttt tctattttca atgaaagacg
     3361 aaccacttcc tttaccaccc ataccaggaa catctccagc acaagctaat ccattacaaa
     3421 ttttacatac tttacatgtt ccattaaagt tctctctagc tgattttagt aagtcattgt
     3481 aattcataca aaatctctcc tttttataat attaaaattg agtattacta tattgaaaat
     3541 atcttaattg taaatatagg gatgttatta tgatgagcta aactagataa tacatattag
     3601 atatttttta ttgttgtaga tagttaaatt atatcatatt agatagtcaa attttgaaaa
     3661 aaactaactt atttaagtat acattttgaa aaaattaaaa aagctatata tagattttta
     3721 aagaaatcta tatatagctt ttaagtttat gtcaatcttt tttgttaatg aataaataat
     3781 atagaataca atatacaaaa aagtttcttg aaatgctaag ataatcccca tttacaaagc
     3841 aacatcatta tactgtgtaa atttgaatca acaatgtttt caccatattt catcataaaa
     3901 tcctctaatt gtgtgtcaat aaatgaataa tatccactta tactatcgat gtatgcagtc
     3961 attaagtgta tacattcatc acttttatct ttatacatat atatctcccc ctatatacaa
     4021 tatataaatt tagataccaa atcgtatcat attaaatagt taagtgtgta acatctatgc
     4081 agttatatat ttatatcatt atattaataa ctaaagtgag ttttgacaat agattttggg
     4141 aaactggatg ttcaaagtgc aaacttggat tttaaaatca ttaattaaga tagcgcatag
     4201 gaatatatgt atacataact taattataaa gattataaac aattactttt attaacttta
     4261 tttaaatggt agaataactt ggagattaat aattataagg aggatataat atgagtttat
     4321 ataagtttat atatgatgat aaggaatatt tattaaaaga ggagaactgt agtgctttaa
     4381 taaatgatga agaaaatcca gttcaaggag taagtatttc aaaaataatt gatatactta
     4441 atgaagcaga agaagttgat tttgatgtag aatattatca agaagcatgt ccattatgtt
     4501 tagaaggagt aaaagaaaag aaaaaattct ttcctttttt agaatatcac ttttatattt
     4561 tcagtaaaga tggagaatat gtaataagca atatatctgt tgattataaa ggactatctt
     4621 ttaataaact atcaagggca aataaagttg ataatagtta tattgtaagt gtgataattt
     4681 gcgaaaattg tcaggattat ataattcaga tagaaaattg tatagtttag tgtaaagtat
     4741 gtcttaattt aagagaaatt tctaaattga gacatcaact tttttcaaaa atagtatgga
     4801 aaattactaa atttatgaga taatgtaagt cggaggtgag ttacaatata tagtaaatat
     4861 ttggaagaga tagatgatac aattttagat tcaatgttta aactaacctt gaatgaaaga
     4921 ttattgacta ataacgattt tattgaaaat aaaattgatg tagtaaagaa tttaattgat
     4981 tgctataaaa ctgaacaaaa tagtttaatg ttattaaaat caaatttttt gctagcttta
     5041 ttatatggta ttcaagggtt tagtgaaaaa atgaaagagt atattttgat ttcctgtaaa
     5101 tatattgata aatgcttacc caaagattat aaatttttag cacgatttta ttcacaaata
     5161 gctattttaa gtttaaaaaa tgaagatgta aataaggcaa atttatatat ggataaattt
     5221 aatcttattt gtgatgaaaa taattttcta attgaagaaa ttatttttaa atcacaactt
     5281 atttacatta aggcaagtaa gtgtatagaa tcttctgtaa taattaaaga aatagaaacg
     5341 ttgtatataa agataaaaga aattaacgat tttaattgta aaaaaatata tttttttatt
     5401 attggtaaaa tatatttttt gtttttggat gatgctctaa ttgctaaggt aaatttttta
     5461 aaagcaagaa aatatgcaga tttactaaag gatatgcaag tatcttcact atgtaatata
     5521 aagataggtg agtgtgaatg ttcgtttgaa aattatgaat gttccatgga atatttcaat
     5581 gaagtaataa gcaataaaaa gtatagaaat gtaaatataa ttcaaaaata tagagcatca
     5641 aacaacataa ctaaaatatt aataaagact agaaattatc ctgtcgcaat aaataattta
     5701 gcaaaatctg aagtatatct agaaaaaatt aaaaatcata gcttaaaaga agtagagaag
     5761 tttgatttat ttataagttt agccatgtat tatgcaaaaa gtaatgaaaa atcttttgaa
     5821 caatcaatat gctatttaga tagagctaaa aacatattaa atatgataaa aaatatagaa
     5881 gaatatataa tatatgattt agaaataatt tatcaccagg caaagatata ttatatcttt
     5941 ggatattatg acaatgcctt aaacacaaat aaaaaactcc ttgaaaaatc taaaaatgct
     6001 aaaaattata attatgtgaa gttagcatac aagagtatat atttgtgttt tgaaaagatt
     6061 caaaattatg aaatgagtat gaaatacttt aaaatgtatt atgagttaaa aatgatgtat
     6121 ataaaagctc gaaatagaaa ttatatagat tccttaaatt atagacatga gtatatagaa
     6181 aaagaaataa ataacatgag aaaagtaaaa cttgatttag ataaaaaaaa gtatgttgac
     6241 catttgacaa gtgcatataa tagaactttt ttgaacgatt ttttagaaaa acaagaagtt
     6301 agtgaatatt gcacagcatt tatgatagat gtagattact ttaaagaata caatgatact
     6361 tatggacact ataatggaga tattgctctt aaaaatataa ctataataat aaaaaaatat
     6421 ctaaaaaaag acatgcttat gataaggtat ggtggagaag aatttctgat attatctgta
     6481 tgtaaagatt ataaaaaaag taaaatattt ggaagaaaat tatgtaggat tgtaaagaaa
     6541 tttttaaaca ataatttgac agttagtatt ggcattgata catgtaaaaa tagtagtata
     6601 gatataaaag aaataattga aaatgctgat aaagctttat ataaagcaaa ggaaagtggt
     6661 agaaatagat gtttgcatta tcatgatttt aagaattttt aactgttgaa ataggattgg
     6721 tgatttactt gaaaaggagt ttaatagaag atttaaagag aaatataaac aagatagaat
     6781 acgatatgtt gactaaattc tatctaaatt tgacagattt aaactacatc ttggattatc
     6841 acaaagacct tttaaataca aaattagaag atgatagtag agtctatact ttatatttaa
     6901 ttagtcttat aaactatata aaaaaagata gagaagcatc atacaactac ttgatagagt
     6961 cattaaagta tattaaaaaa ttgtcattaa aagatgattc agatataata tctaaaattt
     7021 atatttatct tactttatca gctatttcta taagaaaata tgataaggta aataattttt
     7081 atttctccgc aaaaaaaatt ataagacaaa atcatttaaa tgagttgttg gtattgttaa
     7141 atatgagttt atgtgtggaa ataagtgctt tatatcagac caaaacggat gtaatagctt
     7201 tagtagaaga agtgtctttt tttaaaactt taaaaaataa agctcttgta agtagggcaa
     7261 attatatatt tgggagagtt tatctatatt tatttaataa ctttataaaa agtatggaat
     7321 acttaattga atgtctaaaa ttggctcaag aaaataattt atactctata gaggcttttt
     7381 gtaaatgtat aataagtttg tgttatttag agagtggaaa taatgtagaa gctataaaat
     7441 acataaataa tgtattagat gttgcacaca gtaataactc aatatcagta actgaaagag
     7501 tgtctataaa aatagacctt atatgggcat atttaaaaga agatatgaat aaagaagcag
     7561 aagttatttt attaaagact ataaaattaa tgaaagttgt tgaaggtgta tataaggatt
     7621 atttatattc atttatattt ttatattcag ctgaaattga gttaaagaaa gataattgta
     7681 gttttgaaaa ggtaaacacc tatttaattt tatcaaaaaa tatatataaa agttttggag
     7741 atgtttttat atataattct aaactagatt acatatataa gttgtatgga gatttatata
     7801 taaaatttgg taatatagat gaaggtataa gttgttatat aaaaggttta aatttagtta
     7861 aaaagagttc attgaatctt agaaaaatat ctatatttta tgggctaata gcaaatggat
     7921 atgagctaaa aaaagatttt aaactggcaa tagaatatta taaaagtatg gattggtatt
     7981 ctgataggtg ggaaaaaaat aattcctata aaacatctca agcaattcac aaacaatatg
     8041 aactcagaca aaaacaagag agtttaagat tactagtgga tgataataag aaattagaaa
     8101 atgatatttt aaaagatgga cttactaaac tatataatag aagatatctg gaacagcagt
     8161 tggatttatt taatggaacc agagacaaaa atatggtcgc aatactggtc gatatagatt
     8221 gttttaaaag ttataatgat aattatggtc atctagcagg agataaagtt atagttaagg
     8281 taactgatat tataagaagt gtatttatag atattaccga acacataatc agatatgggg
     8341 gagaagaaat tttaatatta gtagattcta tagaatcaat cggtgttgaa gagattgata
     8401 atatagaatt atatattaaa agagttttca aacttcttga atttgagggt attgaacata
     8461 tatattctaa aataaaaaac tatataacta taagtgctgg agtaagtata aaaagatgtc
     8521 gtagtagaaa agatgttgaa atattaattg aagatgcaga taagaaccta tataaatcaa
     8581 aaaaagcagg tagaaatcaa tacattataa tttaaaattg aggtgatttt gttgcgaaaa
     8641 aaaataggaa ttatagcctc tgatattgaa ttaaaagaaa gaatagaaga attatataga
     8701 gaagatgtag aaaatggaac tataataatc gacatactta acctagactt aatggaaaat
     8761 caaggtcgta tacttgttga aaaaggtgca caagctataa ttggtagagg tggaggttat
     8821 agtttagtaa tagatacagt aaatgtacct gttataccaa tgaatatgaa gtcaactgac
     8881 cttttaagag caatagaaat agcaaagaaa tattctaaaa aagtagtatt gattcttgga
     8941 gacaatgaag tttcttttga ctatgttggc tggagaaatg tcataagtac tgaaataact
     9001 gaagaatggt ttgaatctaa gtatgagata agaagtaaag ttgtaaaata tatagaccaa
     9061 aaagatgaag ttgtaatcgt aggaggaggt ttggcatgtt cttttgcaag gcaatatgga
     9121 attgatagtg tatttgcaac tgctagtgat gaatctataa gagaagctgt agaatattgt
     9181 aaaaaattat tagatacatt aggtgaagaa aagtttaata atgaagtgtt acgaaatata
     9241 cttgatggta taaaagatgg agtaattgct atagatagta atggcagtat aattttgtac
     9301 aatgaaagtg caaaaaacat gcttaaagta gagagaaaat gtgcacttaa taaatacatc
     9361 ttagatgtat ttcctaaaat ggagtggatg ttagattgtt tacatgaaaa agaggacgta
     9421 gaagatagaa agataagaaa tataaataat ctaattgtta atactaggac aacattgata
     9481 aaagttgata atagtactta tggagtttta ggaataatac aagatataac caagctacaa
     9541 aacttagaaa gaaaaataag atttgacttg aatcaaaaag gtctctatgc tagatatact
     9601 tttgatgatt ttttatttaa agataaatta actaaagaat ttattgaaga agctaaaaaa
     9661 attggaaaaa gtgattatac aactttactt tatggagaaa gtgggtctgg taaagaaatt
     9721 atagctcata gtatacacaa cataagtaaa agaaaagata ggccttttgt tgctataaat
     9781 tgtgctacta tagcagaaaa tttacttgaa agtgaacttt ttggctatga agaaggtgca
     9841 tttactggtg ctagaaaggg tggaaaaaga gggctatttg aacttgctca tggagggact
     9901 ctttttttag atgaaataaa tagtctatcc tttaatattc agacaaaact attaagagta
     9961 atagaggaac gtcaaataat gagaattggt tctgattata taatacctct ggatataagg
    10021 attatagcag caacgaacga atctttaacc gaaaaaatag ttatgggtac atttagagca
    10081 gacttatttt atagattgag tagtttagag ataaatatac ctcctttaag agacagaaga
    10141 gaagatataa tacctttgtt taacaatttt gtaaatgaag tattaaagga tgatggtctc
    10201 aatggaataa acagtataga tgaaaatttt gttttgacta aagatgaaat ggataagcta
    10261 tataattata gctggcctgg aaatgtaaga gagttaaaga caatagctca aaaatatgtt
    10321 gtgacaggta aaattaaatt gagacaagat agagatttta aaactaaaaa attacttcct
    10381 aactctgaag tagacaaatt taattctgaa acgacagcaa gtgtagaagt ccaagatgaa
    10441 tctataaata taagtaagat aaatgatggt aagataagta tagatataaa agaagtaaat
    10501 aagtatgttg aagaaaaaat aataagtatg ctttttgctc aaggactttc taaaaatgaa
    10561 gttgcacaag ttcttggaat aagcaggaca tctttatgga agaaatacaa taaaaatatt
    10621 taaatataat tacatataaa ttattttgaa gtaatgttaa ttttttaaaa caagtgttaa
    10681 gaaaaattaa cattactttt ttgcaaattt ttattttttt agacaggcat atatatttat
    10741 aagaaaaata gttaaaacac ttaatttaat aatgaaaaaa ataataattc taaacaaaat
    10801 caattttaca taaagtggga tttattggca tagtaattgc ttaatatata atgtaagagt
    10861 aaatacaatt ttaggggtga tggtatgagt caaagtatgg ttggtaagca tgcaatgtgg
    10921 ccaaaagaaa atgatgttat atttagcata tctggcagag ctcaagcagc agagaaggct
    10981 tttggtatgg ataatgtaat aaatgcaaca ataggcgctc taatggatga ttcaggaaaa
    11041 ttaataacaa tgaaaactgt atatgaagaa tataaagcat tagataactg tgagataggt
    11101 gcatatgcag ctcttgaagg tcaaccagat tacttagaag cagttaaaaa agtatttttc
    11161 agagattact taccagaagg acatataaga gtattagctt caccaggagg aagtggtgca
    11221 ataaaattag ctgtatggaa ctatactaat gaaggagatg aggtattaac atctgattgg
    11281 ttctggagtc catatgtaag tatagctgaa gaggctaata gaaaagtagt taattaccaa
    11341 ctttttgatg aaaatagaag gtttaatttt gagtcgttta aagaaaaatt tgtaaatata
    11401 gcagaaaaac aaggtagagt ctttacaatt ataaacacac cagcgcataa tccaactgga
    11461 tatagtgttg cagatgatga gtgggataaa atattagact tatcaaaaga agtcgcaaaa
    11521 gataaagaca aaaagataat atttttcgta gattcagcat atattgactt tgcaggtgat
    11581 gatgatgtat gtagaaagtt ttttaagaaa ttttctaatt tgcctgaaaa cgttctcgta
    11641 ttggttggat tcagtatgtc aaaaggattt acagcttatg gtatgagaat gggggctata
    11701 atttgtatat cttcaagtga agatgtagct gaagagttcc attactcatg tgttcattca
    11761 tgtagagcaa attggtcaaa ttgtaataga agtgccatgg cagtattatc taacattgta
    11821 aatgacccta aaaaattcaa agaatatgag gatgaaaaag aaatatacaa aaacatgctt
    11881 actagaagag cagatgtatt tgtaaaagaa gctgaaagag taggattaga aattcttcca
    11941 tatatagcag gtttctttgt aagtatacca tgtgataatc caaaagaagt atgtgaagaa
    12001 ttgactaaac ataatttatt tgcagttcca ttaaaaatgg gcttaagatt tgcagtttgt
    12061 gcagtttctg aagataaatg taagaaagca ccaagtatta taaaagaagc acttgaaagt
    12121 ttagaagtaa aaataaataa ttaatttcat tgaacaaatt gggtggacta tgaatttaat
    12181 aattgtagat ggagggaatt ttatatgaaa caattgatga caggtaatga agcaattgca
    12241 cgtggagcct atgaggctgg tgttaaatat gcatctgcat atccagggac tccaagtaca
    12301 gaaatattag aaaatatagc aacatataaa gatgcaatag ttgcagagtg ggctccaaac
    12361 gaaaaggtag cacttgaggc tgctataggg ggttcaattg caggagcaag aacaatggct
    12421 tctatgaaac atgttggttt gaatgttgca gctgatccaa tatttactta tgcatataca
    12481 ggggtaaatg gtggtatggt acttattaca gcagatgagc cgggaatgca ttcatctcaa
    12541 aatgagcaag acaataggat gtatgcaaaa tttgcaaaga tacctttatt tgaaccatca
    12601 actagtcaag aagctaaaga tatgataaaa gaagcttttg aagttagtga aaaatacgat
    12661 acaccagttt tatatagagt tacaacaaga ctttgtcatt caaaaggatt agtggagtgc
    12721 tatgatagag aagaagttga aattaaagag tatgttaaaa atgctaagaa aatggtaact
    12781 gttccagcaa atgctcaaat aagaaggggc gttgttgaag aaagaatgga aatccttaaa
    12841 aaattctcaa atgaaactga tttaaactac tatgaaataa atgatacaaa aataggtgtt
    12901 atagcctctg gtatgtgttg taattttgcc aaagaagttt ttggaaaaaa tgcatcatat
    12961 atgaaactag gatttacaaa tccattacct tatgaaaaaa taaaagaatt tgcagaaaaa
    13021 gttgataaaa tatatgttat agaagaaaat gacccattta tagaagaaca aataaaagca
    13081 tatggaatag attgtattgg aaaagatgta ataccaccat atggagaaat gacaccagat
    13141 gttttaagaa aagctatatt tggaaaaaca aatgatacta tagaatacaa atctgaatta
    13201 gtaacaccaa gaccaccaag tttctgtgca ggatgtccac atagaggatt cttctatgaa
    13261 cttggaaaga gaaaaaattt aatagttggt ggagatatag gttgttatac tcttggattt
    13321 gctcctccat ataatggaat agactatgtt gtttgtatgg gttctgcttt tggaactgca
    13381 catggagctc aaaaagttct taatatgaag gatgataatg aaaaaagatt ggtaggtgta
    13441 cttggagact caacattctt ccatacagga ataaatggac ttttagatgt tgtttacaat
    13501 agaggtaatt ctatttcggt tatattagat aatagaataa ctggaatgac aggtcaccaa
    13561 gaaaacccag gttcaggata tactttacaa ggtgctaaaa ctaaagaagt tgatatagaa
    13621 ggattagtta aagcttgtgg aatagaacat gtaagagtta taaaccctaa taatttaaaa
    13681 gaagtaaatg aagcactaga ttgggcttta gctatagaag atgaaccatc agtaataata
    13741 acaagatggc catgtgtact taaaaaattc tctaaagaag atatagaaga atttaataat
    13801 ccatttaaga ctaagtgtaa agttgaccat gataaatgta taggatgtaa attatgtttg
    13861 aaaactggat gtccagctct atcatttgat aaagagaata agttatcaaa tatagataga
    13921 aatcagtgtg taggatgtgg agtatgtgca caagtttgtc caaaacaagc aatagttaag
    13981 gaggaaaaat aatatgacta agagtatact tttggtaggt gtaggaggtc aaggaactat
    14041 acttgctagt aaattattaa ctatgggatt gatggaagct ggatatgatg taaagatgag
    14101 tgaaatacat ggaatgagtc aaagaggagg ttcagtttcc tcacaagtta gatatggaga
    14161 ttgtgttcat tctccagtta tagaaattgg tggagcagat atattagtat cttttgaaaa
    14221 aatggaagct ttaagatggt tcaattattt aaaaccagaa ggaaaagctt tagtgaataa
    14281 tcataggata gattctatga ctgttttaat cggaggagct gaatatcaag aaagtgatat
    14341 agatgcagag ttaagtagat taaatgcaaa agttataaat gcagctgata aagctgaaga
    14401 actaggaaat tcaaaaataa tgaatgttat tttattagga tgtctagtta aatctatgca
    14461 attagagtct attgattggg aaaaaattat aagtgaaaat gttaaaccaa aatttgttga
    14521 attaaacata aaagctttcc atgaaggtat ggaaatggtt agcaaatagt ttataataga
    14581 tataaataga ctatagaaat taaggtgaaa ggaagaaaat tatgacttac agaatattag
    14641 ccataaatcc aggttctact tctacaaaaa tagcagtata tgatggagaa gaacaaattc
    14701 ttgtgaagac gatagaccat ccagctgaag agattgcaaa atataatact atacaagacc
    14761 agtttgaaat gcgtaaggaa gcagttttga atattcttaa agaaaatagt atagacttaa
    14821 aatctcttag tgcaatagta ggaagaggtg gagttttacc accagtaaaa tcaggagcat
    14881 atttagtaaa tgaagaaatg attgatgtac taagacatag accagtactt gaacacgctt
    14941 ccaatttagg tgctgttgtg gcacatgcaa tatcagaacc tcttggaatc aactcatata
    15001 tttatgattc tgttgcagta gatgagctta tagatgtagc gagaatatct ggactttgtg
    15061 gaatggatag atcaagtgca gggcatgcat taaatactag agcaatggct ttaaaatatg
    15121 ctaaggataa aggaaaagat tataagagct taaacttaat agtagctcac attggtggag
    15181 gagtaagtat ttatcttcat gaaaaaggaa gaatggttga tatgctatct gatgatgaag
    15241 gaccattttc tccagaaagg tcaggaagag tacctgctac aaaattagtg gctgcctgtt
    15301 attcaggtca atattcagaa agagaaatga ctaaaaagat aagaggtaaa ggtggtatag
    15361 tttcatacct aaatactgta gatgctagag aagttgaaaa aatgatagca gaaggaaatg
    15421 aagaagcaaa aattatttat gaagcaatgg cttatcagtt agcaaaaggt attggagagt
    15481 tagcaactgt agtagatgga aaggtagatg ctataattat aacaggtgga attgcatatt
    15541 ctgaaatgtt tacttcaatg gttaaaaaga aagttgagtt tatagcacca gtagaaatta
    15601 tggcaggaga aaatgagttg gaatcacttg cttttggaac tttaagagta ctaaatggag
    15661 aagaagaagc tagaatttat agtgaaaatt aataagaaaa actaaaaatg tttatatact
    15721 aaaaaagtgg tatatccgtt ttttatacac gaaagcttag agtataggtg aaaatgtata
    15781 aaatttggat tgccactttt tataattaaa attttatctt aacctaataa ataattctgt
    15841 aaaatttcgt atttatatat atattttatt ggtatttatt gttattatta ttatgaggag
    15901 tgatttttga tgggaaaaat aagtttaaaa agtatgataa gattattttc aggttttttt
    15961 atatttgcaa tttcttctgt gcttatgatt aatgcacatg taggtcttat gccttgggat
    16021 gtacttcacc aaggtttatc tataaagcta ggcataacta taggtcaggc ttctataatg
    16081 gtaggagttg taattgttat attggatgca gtttttggtg aaaatatagg atggggtact
    16141 ttactcaaca tgacatttat aggaattttt attgatttag taatattttc tggagtgata
    16201 cctcatgcaa gcaacactta tataggtgtg tttatggttg ttataggaat tattttggca
    16261 gctatagcaa gttttctata cttaggtgtt tgtttaggaa gtggaccgag agatgggctt
    16321 atgattgctc ttcagaaaaa aactaataaa tctgtacgtc ttgttagaac aattcttgaa
    16381 atactagcat tagttgttgg gtggttatta ggaggttctg taggtattgg tacgcttgta
    16441 agtgctttag gtttaggata tgtattacaa atagtgttta gaatatttaa atttgacaca
    16501 aaattgctga aacatagatt tattatagat gatataagag aatggaagga aaagaaaagc
    16561 aatgaacata aatgtaaatc aagtattgta ataaaaaatg agcagaatta attatgctaa
    16621 aatatatttg tagtttatct aatttatagt aaagtcaagg agattttgaa atggaattat
    16681 gggatttata taatgctgat ggaattaaaa caggaaatgt tataaaaaga ggtaattctt
    16741 gaaaaaggtt attaccattt ggcagttgaa gtgtggattt taaatagtaa ctctcaaatt
    16801 ttaattcaga aaaggtctaa aagtaaaaag acattgccaa atatgtgggg aatgacaact
    16861 ggatgtattg tttctgggga agacagttta gaaggagcaa ttcgcgaagc aaaggaagaa
    16921 atagggatag atataactaa agatgagatg aaagttttta gaagtatgat tcatgaagat
    16981 actctttggg atgtatactt agttaaaaaa gaatatgata tatctaaagc tattttacag
    17041 gaggaagaag taagtgatat aaagtgggta agcacagatg aaataagaca attactaaaa
    17101 gagggtttgt tttttgaata tccagagata tacgaattgc tatatgagat agataataat
    17161 aaattaaata tatgatgtag agcagatttc agtataagta agattattta aaatgtatct
    17221 tctctaaatt ttaatattgt tttatgaaaa ttaataagtt atttttagta tttttctact
    17281 tgttttttga gattaaaata aaaagggcag tcattataaa gtcataaaaa tttggggagg
    17341 atttaaacta tggaagataa caaaggattg gcacgtgata gttcaaaata taaaattgtt
    17401 tctatatctg tcgtagtaat tattctaatt acattttggt atatgttaaa tatggtgctt
    17461 ttaacattta ttatgacttt tattttctat aacttgcttg tagccacaag aaaacgaatt
    17521 aagaagtttt cttctttaaa tattccagat tcattgataa ttatagtgtt atatgcacta
    17581 tttgctatat tgttagtatt gattagctat gcagttgtac caataattat agttcaactt
    17641 acagagttga gtagggtttt tagtgatttt gatgtaaatc aatttgctca atctttagga
    17701 ccgaaattgt atcctattgt ctcaaagctg gattttaata aatatatttc acaagcaggg
    17761 ctacttatag catcaacagc aacaaaagtt ggaagttttg gagtaaatat attattggca
    17821 tttttactga gtttactcct tttacttgaa aaaaatgaaa ttaaaaattt tggagataaa
    17881 ctttctgata gtaagatttc ttttatttat aattcgcttg tattttttgg aaagagcttt
    17941 gttaaaaatt ttggtgaagt aatgaaagta caagttatga ttgcctttat aaattctgtt
    18001 gtttctatga tttttttagg tttcatggga ttccctcaaa taggggcttt aggatttatg
    18061 atttttgtgt tgggtcttat tccagtagca ggagttatag tgtctttgat tccattaact
    18121 gtaatagcat ttaatacagg aggaataaca aaggtttttg gagttttact tatgatttgt
    18181 atagtgcatg cagtagaaac atacatatta aaccctaagt tgatgtcgaa tagaactaaa
    18241 cttccagttt gttttgtttt tattatttta cttgtaggag aacattattt aggtgtatgg
    18301 ggattactaa ttggagttcc aatatttatg ttccttatgg atattctggg tgttaaattt
    18361 acttctaggt aatatattaa aataataatt tatttttata tataaattat gaattgtttt
    18421 aaaaaagaac ctataaatag acatcattaa taaagacatc tatttgatag gttcaatttt
    18481 gctttgtata aatactataa aattaaataa aaagaaattt atttcatttc aaaagtttca
    18541 caacttgcat ttctttcatt tatatgaact ttttcagctt tgcaaagttc atttttattg
    18601 tatttacatt ttacagcttc acaatgaatg ttatctgtat caacttggtt agtttcatta
    18661 gaacgactag ttataccaga tgtatatttg tcttcaaaag aagcacaata agtattttct
    18721 gtagtagttg cttgcattcc atctactttt atactaccag cataacaata tccactagta
    18781 ttgtaagcac aattagtagc agcacaattt aaattattat ttttcataaa taacctccta
    18841 agttttattt aaacttatta tgtgaagtta tctaaatttt atacacttat ttacaattac
    18901 atttatcaca tttaacaact acatctttgg aagtttccct tcctaaattt ttattaacat
    18961 gatatacatc aacattttca gttgatccac atatagaaca tttaaaattg ttttctagta
    19021 gggttctttc tttaaataac ttccaataca tagaattgat gtaatcatta taatttacct
    19081 tgaggcatgt ctcaagaaag tttatgtata gattgtctaa atctcctgta tttaattctg
    19141 tatattctgg ttcaaaatat tcacaacatc ctctaaaatc aggctttgta tctgaaataa
    19201 cacatctaat atcatatcta tagtctaggc taggcccctt aaatgaaaga tgtgtgcaaa
    19261 aagaacaaga tgtttctggt agattttttt caagttcatg taatttatta ttcataagta
    19321 ttcctccaaa aaatttattt taagtatata aatgaataga ttattttaaa acttttattt
    19381 atttatatta gagatttagg ataaatattc caataaggag taaatactta agtatttatt
    19441 actgtattaa ataaaaaaga ggcaaatcta acttgagatt gcccactttt ttatcatttt
    19501 ttaataaaat ttattctaag tattatttta tttagattta gtcagccaac ttagtatctg
    19561 ctttgctttt taactttcta ccagagcgta cagttaaaat agcatataat acagtaaata
    19621 tggcagctaa aacatatgat atatttatgt ttaagttaaa tccaatttga gcattaaata
    19681 tataactgaa tataacaaag gaataaaaca taccaggaat taagcttata atataattct
    19741 tttcttttat tataaggtaa actgtaatca ttgcaaatgc aaatatggca atagtttgat
    19801 tactccaaga gaagtatctc cacaatatat taaatccaga agcactaagc tttgcaaata
    19861 taaggatggc tatgacagga ataaatatac agattgacaa tataacacga tgtttctttt
    19921 ccttttggtc ataatgaaag taatctgcaa gcattaatct taaagatctt aaagctgtat
    19981 ctccagaagt tattggtaaa acaattacac ctattatagc tatgatacca ccaatagaac
    20041 caagtaaatc tcttgcaacc aaacctatta catcaggaga acctactaat tctttaggta
    20101 ttcctttatt gtaaataccc atagcagctg cagcccaaat cattgcaatt aaaccttcta
    20161 aaatcatcat attataaaaa gtagtttttc cttctttttc atttgaaact gatcttgcta
    20221 taagagtagc ttgagttgag tggaatccag aaacaatacc acaagcaact gtaacaaaga
    20281 atgtaggaat aagaggaata ccatcaggat gaataccttt ccaatttgca agacttaaat
    20341 tagctaaatc ataaccttgt gtaaagattc caattccaac accagcagca gataaaagta
    20401 gaatagctcc aaatattgga tatacctttc ctatgatttt atcaattgga aatagtgttg
    20461 cacacaagta atatagaaag ataagtccat aaactatcca gacaactgga ttatttatag
    20521 tcgatttcat atttaaaatt tgagtaacaa ctaaatcccc aggtgtataa ataaaaactg
    20581 caccaactaa aagcattaaa agacaaagga atatattata gacttgaaat actttattac
    20641 ctaaaaatct actgataaga gaaggcattt gtgctccttt ttctcttata ctaatcattc
    20701 cactcatata gtcatgcaaa gctcctccaa atacacagcc aataggtatt agtatgaatg
    20761 ctattggacc aaacaatata ccttggatag gtcctagaat tggtcctgta ccagctatat
    20821 taagcaactc aattagacta tttttccatt ttttcattgg gacatagtct actccatcac
    20881 tttgagcaag tgcaggtgtt tttctgtcat ctgggccaaa tactttttca caataagcac
    20941 cgtataaagc acctcctatt agtaatatag ccaagccgat taagaatgtt gtcataatat
    21001 caccccattt ataaaatttt ttggtctttg tatacatatt atctaaatgt gtagatttat
    21061 attgaattta attctggaca acctacaaat tctataaagt attacaacta atataatttc
    21121 agaaataaat acatatacat aaccatgtac ttagtataca ggtatttggt aatattttca
    21181 atgttatagc aataaagaat ataaacaaaa ttctgataat attttacaaa aaaattaaaa
    21241 aatataaaaa ttagtttaaa tagatggaga aaaaaggaaa atcaattgag aatataaatg
    21301 tctatatctt aacaatatta agaggtttaa aattcttgaa gaaagaatag tcaagtgata
    21361 taatgagttt acaggtgtat atacactagt acatatagaa agcggtgcaa ttatggataa
    21421 agatttaaca tatcaaatca aagagctaaa gaaagaaaaa aatgcaataa tacttgccca
    21481 tttttatcaa ccaccagaaa tacaagaatt agcagatgca gttggtgact catattattt
    21541 aagtgagatt gctagagatt gtaaagaaga agtggttgta ttttgtggag taaggtttat
    21601 gggagaaagt gctaagatat tatcacctga aaaaactgta ttgatgccag taagtaacgc
    21661 tgggtgtgct atggcagata tggtagatga agaaggcgtg ataaaattaa aacaacagta
    21721 tccaaatgct ttggttgtat gttatataaa ctctacagct aaagttaagg cacattgtga
    21781 tgtttctgtt acatcttcta gtgcaattaa aatactagaa aacatagata ataaagaaat
    21841 tatattcctt ccagataaaa atttaggagg atatattgca gaacaatttc cagataaaaa
    21901 ctttatattt tgggacggat attgtaaata ccataacaat ataagagctg aagaaataat
    21961 tgagttaaaa gataagtata aaaatgcaga agtattagta catccagaat gtaaaaaaga
    22021 aataagagat ttaggagatt atgtaggtag tacatcagga ataataaaat atgctactaa
    22081 tagtaaaaac aaagacttta taatagcaac tgaagaaggt atattacatg aattaaagaa
    22141 aaataatcct aataaaaact tttatattcc tggtggaaaa atactatgta cagatatgaa
    22201 aaagacaaca ttagaaaatt tatatagtac acttaaaaat atggaaaatg aagttattgt
    22261 agaagatgaa atcatggaaa aggctttaaa ttcactgctt aacatgcaca aattagcaga
    22321 aggatagagg gatagactta tgaatttaga acaagatgtt ttaatagttg gaagtggagt
    22381 ttcaggatta tactgtgcat taaatttaga taaaagttta aatgtgctag tagtatcgaa
    22441 atcaacaatt gaaaacaaca atacatatct tgcacaaggt ggaatttcaa ctgctagaaa
    22501 tattgatgat atagaatcct ttgtagaaga tacaatgaag gctgggcagt ataaaaatag
    22561 agttgaagca gttcaagtac ttgcagatga atctatagaa aatgtaggtc agatagtaga
    22621 atatggtatg cctttagaca aagaaaatgg agagatagat tacacaagag aaggagcaca
    22681 tagtgtaaat cgaatagttc atagtaagga taatactgga gaagttgttt ttaaaacttt
    22741 attaaaagaa gctaaaacta gagaaaatat aactcttata gaagatgcat atcttttaga
    22801 tatattaaaa gatggcaata aatgtatagg agctagaatt tttaaatcta aaaaagagat
    22861 tcatgttttt tcaaaaatag tagttttagc tacaggtgga attggtgggc tttttaaaaa
    22921 ttctactaat caaagacatc ttacaggtga tggaattgca atagctttaa gaaataatat
    22981 aaaaatagaa aatttagaat atattcaaat acatcctaca gccttttatg aagaaaataa
    23041 tgaaggtagg agaatgctta tatctgaatc attaagagga gagggagcta aacttttaaa
    23101 taaaaataaa gaaagatttg tggatgaact tcttcctaga gatgtagttt caaaagctat
    23161 ttttgagcaa atggaaaaag ataaattacc ttatgtgtat ttagatgcaa cacatttaga
    23221 tagtgaatat ttaattaata gattttcttt tatttataat gaatgtttag ctagaggaac
    23281 agacataacg aaagaatgta taaaggtatc tcctgcacaa cattatttta tgggcgggat
    23341 acatgtagat ttggattcaa aaacatctat ggaaaattta tatgcagttg gtgaaattag
    23401 ttgtacaggt gttcatggag ccaatagatt ggcaagtaat tcattacttg aagggcttgt
    23461 attttcaaaa agagcagcta aaaatataaa ctcagttata gataatgtaa aagttaagtt
    23521 tatagatgta ccagatatgg atatagatat agaacaggta aaaaaagaaa ataaaatatt
    23581 agtcataaaa gaaattgaaa gaacaagtga ggattttggc gatgaattat ttgattattg
    23641 atagaatgat acaggatgct ttgatagaag atgttccaag tgaagatata acaactaatt
    23701 ctattgtgga tgaaaattct aaatctactg tagatttaat ttgcaaacaa gatggtataa
    23761 tagctggctt aggtgtattt aagagagttt ttgagatact tggtgatgta gatgtaaaat
    23821 tatataaaaa tgatggtgat agagtaaaga atcgtgaaaa gatagctgta ttaacaggta
    23881 gtactagaaa cttacttgta ggagagcgtg tagcattgaa ttatttacag agaatgagtg
    23941 gtatagctac tattacaagt aggtatgttg aaaagcttga aggtacaaat acaaaattat
    24001 tagatagtag aaaaacgata cctaatctta gaatattaga taagtattct gtcaaagtag
    24061 gtggaggatg caatcataga tttaatttat ctgatggaat attgcttaaa gacaaccata
    24121 ttggggctgc tggtagtgtt aagaaggcag ttgaattagc tagaaaaaat acttcttttg
    24181 ttagaaagat agaagtggaa gtggaaactt tagacatggt aaaagaagca atagaagcta
    24241 atgcagatat aataatgttg gataatatga gtttggaaat ggcaaaacaa gcagtagata
    24301 ttataaatgg aagagccatt gtagaatttt ccggaaatgt aaatttagat actatagaag
    24361 atataggaaa aattggtgtg gatgttgttt ctgtaggagc gcttacacat tctgttaaag
    24421 ctctcgatat aagtatgaaa aatttaagaa atatataaat gataaaaaga ttacctaaat
    24481 atgacagaca tatttaagta atcttttttg taatttgcat attataacct gttttataga
    24541 ttttaatcta caataatatt tagatgttag tatactaggt ttattaatta tttatatagc
    24601 cttattaagt atatatttat ttataacctt tgcaactcca ttttcattat tagtatcagt
    24661 aacataatct gcaatttcct ttatactatt acgtgcattc cccatagcca caccaagacc
    24721 tgcatacttt atcatatgtt ggtcattttc ttcatcgcca acagcaatag cattttctat
    24781 aggtatattt agattcgtac ataatgtttt aagagcagtt cctttattag tagttttacc
    24841 taaaaattct agataaaaag gagcagacct tactatagta tatttattga ataaattttt
    24901 aggtagttgc tgtatgattt cttccaatct ttctggttca tcaactatca taattttaca
    24961 gattttaata gattcatcaa ttttatcttt ttgtatatat ttgacttcta tattatttaa
    25021 tttcgcttca tgaagtgtat atttgccagg cgtttcattt ggtgtaatta aatttgtcat
    25081 tgtatttatg tgtattttag tcttatattt tttacaaacg ttattatata taaaatcaaa
    25141 atcatctaaa ttcatttcta catcatgtat gattttctca cttttaactt cttgaataat
    25201 tgctccatta aagcatataa cataatcacc ttcatctgtt aaatttagtt gatttagaag
    25261 ttcttttaca ccaggaagag gtctgcctgt tgttataact acttttgccc cagccctttt
    25321 tgcctcctga atggactcaa agacttcagg agtaatcctt ttttgtgtat ttaatattgt
    25381 tccatcaatg tctagtgcga ttaatttgta catatcaacc tccaataaat tatatactca
    25441 aatttcctgt gaaaacgttg acacagctag tgttgaacaa agtttcagtg ggaaatatgg
    25501 tttgttttgt atactattat atcaagagat aagtaattta gaaagtttaa taatattaat
    25561 tttttgataa aaatattact tatgtgagat gagaatgtaa aaactttgta atcaataatt
    25621 ccaaaattct taattacaaa taatggtatc gtttgcattg ttaatataaa taaatgtata
    25681 ttatatagtt agaagtttga ataaatagac tgatggtaat ttgttatatt agttagtttt
    25741 gtgttataaa atattgtaat tccattaatc atatgttatt attcaacatg attataatat
    25801 aaagaatatt tgatatatat aataatttat aaaagagtat tacatagtac atgtaaatct
    25861 atatttataa atacaataaa gtaaagttgt aaaggagtgc tttaaatgaa gttttcagag
    25921 tttaagtatg aaagacccaa ttataatagt atgaaaaaag agtttttgag ttgtgtagaa
    25981 gatataaata attctcgtaa ttataaagag caacaaaaaa atatacataa gattaattta
    26041 ttaagaaata aaatagaaac gttgtcaaat atagcttcga taagatatag tacagacaca
    26101 tttaataaat tctacaaaga agaaaaaaat tattgggatg aatatatgcc tttgtatgaa
    26161 gaattaaatt catattttta taatgccatt gtaaattcaa aatttaaata tgatttgata
    26221 aaagagtttg gagagcaatt tttcacaata gtagagtatt ctttaaaaag tttttccaaa
    26281 gaaataataa gtgaattgca ggaagaaaat aaattgtgtt cagagtatac tagactttta
    26341 gcttcagctg agataatgtt tgatgggaaa ataagaaatt tatctggtat ggggaaattc
    26401 atgtattcta agaatagaaa aacaagagaa ttagcaaaca aagcatatta taatttcttt
    26461 gaagaaaatg aaactaaatt tgatgacata tttgataaat tggttaaact tagagataag
    26521 atgtctaaaa aattaggttt tgaagatttt gtagagcttg ggtatgttag aatgatgaga
    26581 agtgactaca gagaatatat gataaaaaat gttagaaaac aagtactaaa atatgttgtt
    26641 ccaatggcaa atgagcttta tgaaaaacaa gctaaaagaa ttggtctaga atatttaagt
    26701 tacatagatg aaggcgtgga atttttaaca ggaaatgcaa gtttaaaggg agattcaaga
    26761 tacataattc aaaatggtaa aaggatgtat tctgaactgt caaaagaaac aaatgaattt
    26821 tttgatttta tgttagaaaa tgaacttatg gatttagaaa cgaagaaagg aaaaggagct
    26881 ggagggtatt gtacttatat accagattat aaatcaccat tcattttttc taattttaat
    26941 caaactgctg atgatataga tgtgttaaca catgaggcag gacatgcatt tcagttgtat
    27001 atgtctagat ggattgatat gccagaaatt aattttccaa cgcttgatag ttgtgaaatc
    27061 cattcaatga gtatggaatt tataacatgg ccgtggatgg atttattttt taaagaagat
    27121 acagataagt acaaattcac tcacttatca tcttcaatta aatttatacc ttatggagta
    27181 attgttgatg aatttcaaca ctatatttac aaaaatccta atgtagataa gtcaaaacga
    27241 aaagaaatct ggagattttt agagaaaaaa tacttacctc atagaaaata tggtgataat
    27301 tcttttttag agagaggttg ttggtggttt aaacaaggac atatatttaa aaatccgttt
    27361 tattatatag attatgtatt agctcaaatt tgtgcgttac aattttggaa aaaaatgatt
    27421 caagatagag atgcaggatg gaaagattat ataaatatct gtaaagttgg aggaacaaaa
    27481 tcatttttag atattgtgag tatgggaaat ttgtattctc catttgatga tggctgtatt
    27541 gagtctatta taggtgatgt taaatcttgg tttgatgaaa taaatgacag caaattataa
    27601 aaaattatga gaattttatt gtataaaagt gaaaaattaa aaaatatata aaaagtgatt
    27661 atttgttgtg aaaatagtct tgtttttaac taaattgata aaataaacta aaaaagattt
    27721 gtacttaaaa aaaaaagaaa atacaagaaa tatgcaatat caacatttgg aaaaaaaagt
    27781 aaaaatgtat acaaaataca tataaaaaaa ctattgcaaa ttttatagaa tgtttatata
    27841 cttaagtcaa cagagataga aaaaagctat ttatagaaaa caaaggataa attgcttaat
    27901 tttaaatttt ctgaaaaaaa taataatccg gatgaagagt atgggagact gctttataaa
    27961 aagcgaccga agggacaagt ttaatcttac ttacccaaaa gattagatga aattctcagg
    28021 taaaggtgac tgtacttgga cggggctctg aaaatgattt taacagggaa gacaaattgg
    28081 gagatttgtt tttcctgttt tttatttatc aagtaataaa taaaagtttg aaagagagta
    28141 aaaaaatgat aataattacg aataatccaa aagtaaaaga agaagttcaa ggcagagaag
    28201 ttttgtttaa agacacaact tatattggaa tcttagaagc aagtagagat ttgatacatg
    28261 aaggttatga acttttatct catccactct atgggagtgt aaagccaaat gaaacacctt
    28321 acaggactgt tatactaaag aaaggaaatc gtttggatat aaactcttta accttaattg
    28381 aagaagcaat catcactgca agtaaatttc agaataataa aaagactcca aaatggacag
    28441 aaagtgttca agatgatttt agagtaattg attatgatat attttataat acaatacaaa
    28501 gaatgcagta tgaataaaaa aatttgttaa aaagttatta aaacagccta aatattaaat
    28561 ctaatttaat attaaataaa atgagaaatt taattgggga ggataagtca aaatggaaaa
    28621 tgtgtatgac ttagtaataa ttggttcagg accagcaggg cttgcagcag gtctttatgg
    28681 agcaagagca aagttaaaaa ctctaatcct agaaaaagac aagactggtg gacaaattgt
    28741 aataacacat gaaattgcaa attatccagg gtcagttcca aatgcaacag gaccaagttt
    28801 aatagctaga atggttgaac agtgtaaaga gtttggagca gaaatgctaa gagacaacat
    28861 tgttgatact gagctagatg gagatataaa agttttaaaa ggggaaaagt cagaatatag
    28921 agcaaaagca gtaataatag gaacaggagc aacaccaaga aagattggtt gtcctggaga
    28981 aaaagaactt actggtaaag gggtttcata ttgtgcaact tgtgatgcag atttctttga
    29041 agattttgaa gtatttgttg taggtggagg agacagtgca ttagaagaag caatgtattt
    29101 aactaaattt gctagaaaag taacaattgt acatagaaga caaggattta gatgtgctaa
    29161 gagtgttgaa gaaaaagcaa aagctaatcc aaagatagag tttttattag atacagtaat
    29221 tgaagaaatc aaaggtgatg gaatattaga atcagttgta tttaaaaata aagttactgg
    29281 agaaactcat gaatactttg cagatgaaga agatggaaca atgggagtat ttgtgttcgt
    29341 tggattagat gctcaaactg atttattcaa aggtaaagtt gatatggatg aaaaaggtta
    29401 cataataact gatgaagata tgagaactaa cataccaggc gtatttgcag caggagattg
    29461 tagatctaag actctaagac aggttgtaac tgcaactaat gatggagcaa ttgcatctat
    29521 agtagctgag aaatacattg atgaaaaatt tgggaactag aatctataaa ttaagaaata
    29581 gtgtctgtaa atttaatgaa taatatttaa aattaaaatt aatatttaaa attaaaataa
    29641 aaatttcaag ggggaataag aaaatgttag atttagataa ggctacattt gaagaagaag
    29701 ttttaaatgc agaaggtttt gtatttgttg atttttggag tgaaggttgt gagccttgta
    29761 aagctttaat gccagatgtt cataaattag ctgagactta tggggataaa ataaaattct
    29821 gtaagatgga tacaacaaaa gctagaagat tagctataaa acaaaaagta ttaggtcttc
    29881 caactatggc tatatacaaa gatggagaaa aagtagacga agttactaag gatgatgcta
    29941 cagttcctaa tatagaaaac atgattaaaa aatatcttta atatgttgat aaattattaa
    30001 cattaaaaga aaaaatctat ttttatctga agataagaag aaaaaggttt ttatgtccaa
    30061 ctttgttaaa aaaataattt taaataaaat aattttttta caataaatat tttttagtaa
    30121 taaaataaag atttaaggca taaaaacaca ataatatatc tttaaaaaaa taagataaat
    30181 tgtaaaatga tataattttg agaaaaactc aaatgcatat acacaaatta aataacagga
    30241 ggtgcaagta tgcgtcttga attagggaaa atctttataa aagatattca atttggtgat
    30301 gtaacagaag taaaagatgg agtattattc atcaataagc aagagatgct acaagaaatt
    30361 ggtggagatg agcatattaa atctatagat atagaattag ctcgtccagg tgaaagcgta
    30421 agaataacac ctgtaaaaga tgttattgag ccaagagtaa aagttgaagg taatggggga
    30481 atcttccctg gtatcatgtc taaagttgac actgtaggag aaggaaaaac tcatgcttta
    30541 aaaggtgtag ctgttgtaac tactggaaaa atagtaggtt tccaagaagg tataatagac
    30601 atgactggag aaggagctaa gtatactcca ttttctaaat taaataatgt agtagtaata
    30661 gctgaaccta ttgatggttt aaaacaatac gctcatgaaa aagctgttag aatgatagga
    30721 ttcaaagcag caatgtactt aggagaagta gcaagaaatc ttactccaga tgaagtttca
    30781 gtatatgaaa ctaaacctct tcttgaatca ataaaagaat atccagagct tccaaaagta
    30841 ggatatgttt acatgcttca gactcaaggt ttattacatg atacttatgt atatggagta
    30901 gatgcaaaac aaatagtacc aactttatta tatccaacag aattaatgga cggagcaatt
    30961 gtaagtggta actgtgtttc tgcatgtgac aaaaacccaa gttatgttca tataaacaat
    31021 ggggttgttg aagacttata tgcaagacat ggtaaagata taaactttgt aggtgtaatt
    31081 ataactaatg aaaatgttta cctagcagat aaagaaagat cttcaaactg gacttctaaa
    31141 ttatgtaaat atttaggatt agatgcagtt atagtttcac aagaaggttt tggaaatcct
    31201 gatactgacc ttataatgaa ctgtaagaag atagaaatgc aaggtgttaa aacagttata
    31261 gttacagatg aatatgctgg acgtgatgga ggttctcaat cacttgcaga tgcagatgta
    31321 agagctgatg ctgttgtaac aggtggaaat gccaaccaag ttgtagttct tcctaagtta
    31381 gataaagtta ttggacattt agaagtagta gatgtaatag ctggtggttc tgatggaagt
    31441 ttaagagctg atggaactat tgaagttgaa atccaagcta taacaggtgc aactaatgaa
    31501 acaggatttg gtcatttaac tgctaaagga tattaagaag tattttatta aacgattgag
    31561 taatgttaat aaaacatatt atagataatt ataataagtg ttatagatat ataaaaatat
    31621 attagaaata taataaggag gattattatg agtttactta gtaataaaaa ggttcttata
    31681 ataggtgacc gtgatggtat accaggacct gcgatagaag aatgtgtaaa aacagtagaa
    31741 ggagcagagg ttgttttctc atctacagaa tgctttgtct gaacagctgc tggggctatg
    31801 gacttagaaa atcaaaacag agttaaagat gctgctgata aattcggagc tgaaaatgtt
    31861 gtgattttac taggtgctgc tgaagccgaa gctgcaggtc ttgcagccga aacagtaact
    31921 gctggagatc caactttcgc tggaccactt gctggagttg ccttaggatt aagtgtttac
    31981 cacgttgttg aggaaccaat aaaatcatta tttgatgaaa gtgtatatga agaccaaata
    32041 agtatgatgg aaatggtttt agaagttgaa gaaatagaag aagaaatgtc tggtataaga
    32101 gaagaatttt gtaaatttta atgcctaaaa gcaaaagggg tgaaactaag agatgggaaa
    32161 actaaaagca gtacattata ttaatcagtt ctttgctggt ataggtggag aagaaaaagc
    32221 tgatactaag ccacatgtgg cagaaacttt acctccaata agtttacaac ttgataaatt
    32281 attaggtgaa gatattgaaa tagttggaac agtagtttgt ggagatagct atttcaatga
    32341 gaatatagat agtgcaagtg aagaagtttt atcaatggta aaaggttttg aaccacaact
    32401 tttcatagct ggtcctgcat ttaatgctgg tagatatggg gttgctgctg gaacaataac
    32461 taaagttgta aaagatgctt tgaatatacc agctttaaca ggtatgtatg ttgaaaatcc
    32521 aggagcagat atgttcaaaa aggatgttta tgtagtagaa acttctgatt ctgcagcagg
    32581 tatgagaaag gcacttccta agatagctaa acttgcagta aaacttgcta atggagaaga
    32641 aattggaact cctaaagatg aaggatacat agctagagga ataagagtta actacttcca
    32701 tgaagacaga ggttctaaga gagctgttga catgttagtt aagaaaataa aaggtgaacc
    32761 atttgaaact gaatacccaa tgccaaactt tgatagagtt gacccaagta aagctgtaaa
    32821 agatttatca aaatgtaaaa tagctttagt tacttctggt ggtatagttc caaaaggaaa
    32881 tccagataga atagagtctt cttcagcttc taaatatgga acttattcta tagcaggagt
    32941 tatggattta acagaagaga cttatgaaac tgcacatggt ggatatgacc cagtgtatgc
    33001 aaatttagat gcagatagag tattacctgt tgatgttctt agagatttag aaaaagaagg
    33061 tgtaattgga aaattacatg agactttcta tacaactgta ggtaatggta catctgttgc
    33121 aaactctaaa aaatatgctt ctgaaattgg agcagcatta gtggctgatg gtgtagatgc
    33181 agtaatatta acttctacat gaggtacttg tactcgttgc ggtgcaacga tggtaaaaga
    33241 aatagaaaaa actggacttc cagtagttca tatgtgtaca gtagtaccta tttcattaac
    33301 agtaggggct aatagaatag ttccaactat agcaatacct catccacttg gaaatccagc
    33361 gcttgaccct acagaagaaa aagccctaag aagaggcctt gtagaaaaag cattaaatgc
    33421 tttaactaca gaagtagatg gtcaaacagt atttgaaaaa taaaaataac acacacatat
    33481 attatataga taaaaatata aacacacact aaaaataaaa aataactata cacaaaaaaa
    33541 ataatataga tttcatgaat ggataaattg gagttattca ttcatgaaat cttaaaaaat
    33601 gccaaaatac ataaacaata atagaatcaa atatacaata aaatacaatg attagagctt
    33661 ttaataagtt taataagatt aaaaaataag tgttcataaa atttatggag gtgcacaaaa
    33721 tgacttatcc agttttaaaa ggggcaggct atgtacttat tcatactcca gatatgatag
    33781 tgcaaaatgg aagtacttgt acagttgaaa gagcaacaaa tccagattca gaatttttaa
    33841 aagaagtaag taatcatata agaagttatg aagatgtagt taactacatg ccaaatcaag
    33901 tttatatagg aaatagaaga ccagaagaat taagagattt accaatgcca tggtgtgaac
    33961 aaaagattga gggaactaga aatggtaagt ttggagaaat aatgcctcaa gatgagttca
    34021 tagctttaat gcaaataagt gatgcatttg atttagtaaa attatcacaa gaatttattg
    34081 atgaagtaaa gccaaaaatc gaaaataact accctgaaat tgcaccattt gtaggtaaat
    34141 taaagggtga tgatatagaa gaaggtaaag aattagtagc aacacacata gctgaaggat
    34201 tataccatga tggaaaattt gttggttatg taaaaagagc acatgatgta gatgttaact
    34261 taaatgccca tacaatgttt gaaaatctag tagtaaaagc atctggagtt ttatctgcta
    34321 ttcaaatgct aagacatagc aaaatagacc ctgctgaaat agattatgtt attgagtgtt
    34381 cagaagaagc ttgtggagat ataaaccaaa gaggtggagg aaactttgct aaatcaatag
    34441 ctgagatagc aggacttcaa aatgcaactg gttctgacac aagaggattt tgtgcagcac
    34501 caactcatgc attaattcaa gcagcagctt tagtaaaagc tggcatacat aaaaatgtaa
    34561 tggtagtagc aggtggagca agtgctaaac ttggaatgaa tgctaaagac catgttaaaa
    34621 aaggtcttcc tgtacttgaa gatgttgttg gaggatttgc agtacttgta tctgaaaatg
    34681 atggtgtaaa tccagttata agaactgatt taacaggtaa gcatactgtt ggaactggtt
    34741 cttcaccaca agcagttatg acagcactta taacttcagg tcttgataga gctaatttaa
    34801 aaataacaga tgttgatgtg tattcagttg aaatgcaaaa cccagatata actaagccag
    34861 caggagcagg agatgttcca gaagcaaact acaaaatgat aggggcactt gctgttaaac
    34921 gtggagactt agaaaagaaa gaattgaaag attttgtttc taacaaagga ttaccaggat
    34981 gggcaccaac tcaaggacat ataccatcag gagctcctta tataggattt ttaatagatg
    35041 atttaactac aggaaataga aatagagcta tgatagttgg aaaaggtagt ttattcttag
    35101 gaagaatgac taacttattt gatggagtat cttttatagc tgaaagaaat acaggtgtta
    35161 ctgaagagac ttcaggaata tctaaggatg aaattaagaa gataatagct gaatctatga
    35221 aaaaactagc attagatatg ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc
    35281 gcagatgtat tcttagaagt agctaatgca atagaaagcg gcgaatttgg caaaaaagta
    35341 aaaattggtg ttactacact tggaagtgaa catggtgtag aaaacatggt taatggtgca
    35401 caattagcaa aatctaattt atttgatatt gtgcttattg gaccaaaagt ggaaactgac
    35461 cttgaagtag ttgaagttaa tgatgaaaaa gaaatgcatg caaaaatgga agaattatta
    35521 gattcaggat atattgatgc ttgtgttact atgcattata attttccaat aggtgtatct
    35581 acagtaggta gagtaataac tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca
    35641 ggaacaagtg ctactaatag aatagaggca atggtaagaa atgccattta tggtattgct
    35701 acagctaaat caatgggtaa caaatgccca aaagttggaa tacttaatgt tgatggagca
    35761 agacaagttg agaagtgctt aaaagaatta aaagataatg gatatgacat ggagtttgcg
    35821 gattcaatta gagcagatgg tggatgtgta atgagaggta atgatttact tgtaggtgca
    35881 cctgatgtta tggttactga tacattatct ggaaatatat ttatgaaagt attttcttca
    35941 tatactacag gtggagatta tgaagctcaa ggctttggat acggacctgg agttggagaa
    36001 gactatgata gaaaggtact tatagtatca agagcatcag gttctccggt agtagcaaat
    36061 gctttaaaat acgcttatga tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa
    36121 tttgcaaagg ttaaaaaagc aaaatttgat gacattatat cctctcttac taaaaaagaa
    36181 gttaaagctg aaaaagtaga agttaaaatg ccagataaag aaattgttac tcgtcaaata
    36241 gctggtgttg atataatgga tttagaagat gcagtatctg agttatggaa aaacggaatc
    36301 tatgctgaaa gtggtatggg ttgtacaggt ccaatagttt tagtaaatga agctaaagga
    36361 gacttagcag ttgaaacttt agttaaagct ggatatactg caaaataagt acatttttta
    36421 tatgtaaata agaacatata gttataattt tattga
//
LOCUS       DAEMHZ010000026        35887 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000026 DAEMHZ010000000
VERSION     DAEMHZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.026"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..1045
                     /locus_tag="KPK72_001195"
     CDS             140..1045
                     /locus_tag="KPK72_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2488430.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1169..2122
                     /gene="msrA"
                     /locus_tag="KPK72_001196"
     CDS             1169..2122
                     /gene="msrA"
                     /locus_tag="KPK72_001196"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG2488431.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            2275..3405
                     /locus_tag="KPK72_001197"
     CDS             2275..3405
                     /locus_tag="KPK72_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488432.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(3522..4490)
                     /locus_tag="KPK72_001198"
     CDS             complement(3522..4490)
                     /locus_tag="KPK72_001198"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG2488433.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            4824..5012
                     /locus_tag="KPK72_001199"
     CDS             4824..5012
                     /locus_tag="KPK72_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488434.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            5272..6471
                     /locus_tag="KPK72_001200"
     CDS             5272..6471
                     /locus_tag="KPK72_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG2488435.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRIEDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            6523..7026
                     /locus_tag="KPK72_001201"
     CDS             6523..7026
                     /locus_tag="KPK72_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2488436.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            7133..7450
                     /locus_tag="KPK72_001202"
     CDS             7133..7450
                     /locus_tag="KPK72_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488437.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            7608..8567
                     /locus_tag="KPK72_001203"
     CDS             7608..8567
                     /locus_tag="KPK72_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2488438.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            8682..9467
                     /locus_tag="KPK72_001204"
     CDS             8682..9467
                     /locus_tag="KPK72_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG2488439.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDEFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            9759..11078
                     /locus_tag="KPK72_001205"
     CDS             9759..11078
                     /locus_tag="KPK72_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2488440.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(11153..11581)
                     /locus_tag="KPK72_001206"
     CDS             complement(11153..11581)
                     /locus_tag="KPK72_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488441.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(11672..13048)
                     /gene="hydG"
                     /locus_tag="KPK72_001207"
     CDS             complement(11672..13048)
                     /gene="hydG"
                     /locus_tag="KPK72_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG2488442.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMNLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(13049..14134)
                     /gene="hydE"
                     /locus_tag="KPK72_001208"
     CDS             complement(13049..14134)
                     /gene="hydE"
                     /locus_tag="KPK72_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG2488443.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(14170..15378)
                     /gene="hydF"
                     /locus_tag="KPK72_001209"
     CDS             complement(14170..15378)
                     /gene="hydF"
                     /locus_tag="KPK72_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG2488444.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(15393..15644)
                     /locus_tag="KPK72_001210"
     CDS             complement(15393..15644)
                     /locus_tag="KPK72_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG2488445.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(16025..17065)
                     /locus_tag="KPK72_001211"
     CDS             complement(16025..17065)
                     /locus_tag="KPK72_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG2488446.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
     gene            17535..18695
                     /locus_tag="KPK72_001212"
                     /pseudo
     CDS             17535..18695
                     /locus_tag="KPK72_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
     gene            19038..19199
                     /locus_tag="KPK72_001213"
     CDS             19038..19199
                     /locus_tag="KPK72_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488447.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            19392..20021
                     /locus_tag="KPK72_001214"
     CDS             19392..20021
                     /locus_tag="KPK72_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG2488448.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVSYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            20260..21519
                     /locus_tag="KPK72_001215"
     CDS             20260..21519
                     /locus_tag="KPK72_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2488449.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            21778..22773
                     /locus_tag="KPK72_001216"
     CDS             21778..22773
                     /locus_tag="KPK72_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG2488450.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            23019..24086
                     /locus_tag="KPK72_001217"
     CDS             23019..24086
                     /locus_tag="KPK72_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG2488451.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDSALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            24620..26530
                     /locus_tag="KPK72_001218"
     CDS             24620..26530
                     /locus_tag="KPK72_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2488452.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(26593..27216)
                     /gene="ytaF"
                     /locus_tag="KPK72_001219"
     CDS             complement(26593..27216)
                     /gene="ytaF"
                     /locus_tag="KPK72_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG2488453.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            27404..28048
                     /locus_tag="KPK72_001220"
     CDS             27404..28048
                     /locus_tag="KPK72_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2488454.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            28262..29320
                     /locus_tag="KPK72_001221"
     CDS             28262..29320
                     /locus_tag="KPK72_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG2488455.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            29424..30608
                     /locus_tag="KPK72_001222"
     CDS             29424..30608
                     /locus_tag="KPK72_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2488456.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            30815..31528
                     /gene="rpsB"
                     /locus_tag="KPK72_001223"
     CDS             30815..31528
                     /gene="rpsB"
                     /locus_tag="KPK72_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG2488457.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            31607..32518
                     /locus_tag="KPK72_001224"
     CDS             31607..32518
                     /locus_tag="KPK72_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG2488458.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            32603..33307
                     /locus_tag="KPK72_001225"
     CDS             32603..33307
                     /locus_tag="KPK72_001225"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG2488459.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            33333..33890
                     /gene="frr"
                     /locus_tag="KPK72_001226"
     CDS             33333..33890
                     /gene="frr"
                     /locus_tag="KPK72_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG2488460.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            33893..34096
                     /locus_tag="KPK72_001227"
     CDS             33893..34096
                     /locus_tag="KPK72_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488461.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            34125..34859
                     /locus_tag="KPK72_001228"
     CDS             34125..34859
                     /locus_tag="KPK72_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG2488462.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            34872..35651
                     /locus_tag="KPK72_001229"
     CDS             34872..35651
                     /locus_tag="KPK72_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG2488463.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT        14142 a         3916 c         5886 g        11943 t
ORIGIN      
        1 gtattaaagg tttgaatgta acttaaatat taaaagagga acattgtaat atgtaacttg
       61 cataaaatat atatactata gtgaaattat tttgattaaa atttaaaata taaggtgttt
      121 tatgtggggg tatatgtata tgaaaaattc taattttaat atattggcat ttgatggtgg
      181 tggattaaaa ggggcattga gtatatccat acttgagagg atagtaaaag agtatccaaa
      241 tctattaaac aatataaata tgtttggagg tacatctaca gggagtctaa ttgcactagg
      301 gcttgcgtat ggtgtaagcc ctaaagagat aaaagaactt tactcaattg aaaattcaaa
      361 gtatattttt aataaaagtt atgcagaaat actcagacca aagtatgaaa acaaaaactt
      421 aaaagaagtt ttattaagta tatttccaga ggaactagaa ttaaaagatt taaataaatt
      481 agtaatgata ccaagttttt atataggaaa tgaggaaaat gcatggaaac cagtatttta
      541 taacaatatg ccaaattctt ttacaaagac tagcaaagtt gtagatgtag caatggcaag
      601 tagtgctgct ccggtatttt ttccaacata taatcgtcat gttgatggag gaataatagc
      661 aactgacccg agtttagcgt gtataattca tgccattgat agtggattta gacttaagaa
      721 tacaagactt ttttcaatag ggactggata tgtatataac agtataaagg cagatactac
      781 cgaatgggga gctattgact ggataattaa taaagaacct gatttaccaa ttatatctat
      841 taccttagaa ggaaattctc aaatgagtca gttattttct caaaaacttc ttggagataa
      901 ttactataga ttaaatccta agatggaaaa agatgtagct atggatgatt gtgatgcatt
      961 agaatatcta atgtctttag gagaaaattg tgatataaaa gatgcttttg gatggataaa
     1021 gaaaaagagc ttttatagaa attagattca atttgcaatt gtatttttac acatgatttc
     1081 attgagtagg taaaaataat aaaatgcaga ttattaagtt acttcttagg gaataaataa
     1141 agtataattt atttaaggag tgatgaaaat gataaaactt gctacttttg caggtggatg
     1201 tttttggtgt atggtaaaac cttttgataa gtatgatgga gttattaaag tagtttctgg
     1261 atatacaggt ggtcatgttg aaaaccctac atatcaagag gtatgttcag gaactactgg
     1321 tcatttagaa gcaattcaaa taacatatga tgatgatata ataagttatg atgaacttct
     1381 aaatatcttc tggaaacaga ttaatcctac agatgatggt ggacaatttg gtgatagggg
     1441 aactcaatat aagactgcta tattttatca tgatgaagaa caaaaaaata tagctttaga
     1501 gtcaagagaa catctggaaa atagtaaaat atttgatagt aaaatagtga ctgatataat
     1561 tccagcaact aaattctacg aagcagaaga aaatcatcag gattattata aaaaaagacc
     1621 attaaactac aatatgtatt ataaagggtc tggaagatat aattttgtaa aaagtcattg
     1681 ggatggaaac aatataaata gggaagagtt aaaaaagact ttaactccta tacaatttga
     1741 agtcactcaa aatgatatga cagaagtacc ttttgaaaat gaatattatg acaataacaa
     1801 agagggaatt tatgttgata ttgtaagtgg agaagtgtta ttttcttcaa aagataaatt
     1861 tgattctgga tgtgggtggc caagtttttc aaagccagtt atagataaat ctatcattga
     1921 gagaagtgat ttctcacaag gtatgtacag agttgaagta agaagtgcta aaggaaattc
     1981 tcatttaggt catgtatttg atgatggtcc aaaagaactt ggaggtttaa gatattgtat
     2041 aaattctgct tctcttaaat ttataccaag agaaaatatg gaagctttag gatatggaga
     2101 atatttaaaa ctttttgagt aattaggaat atttattata aacttatcta aaaggtgaaa
     2161 atataaaaaa atattttcac cttttgtttt tttatcaata gtatgataaa ttattaattg
     2221 taatcttatt tataccaata gattaagaat ttaattttga aagagagaaa acaaatgaat
     2281 ttaaaagact ttaatggaaa gagattaaaa tcagctagaa tttttagagc aaagacgata
     2341 gaacagttgt ctaaagaaac taaaataaat aaaaaagatt taaaagcttt tgaggaaaat
     2401 aagtatgtac caaatataga aaatacatta aaattatcta atatattaaa ttttccaaaa
     2461 gagtattttt ataaaaatga aaatataaat atcgttgtag aggatagcca ctttaaccct
     2521 caatctaaac tacctagagt agaagagatt tcttatagag aaaaaataat tataatacat
     2581 aaaatatatt catttatgga gaattatatt aagtttccaa aagaaaactt gccaaataga
     2641 aaagtaatgt cagatattaa tataaatgat atagaatctc ttgcatataa gactagagag
     2701 ttttgggatt taaatagtac tccaatagta aatatggtaa gtttgttaga gtctaaaggg
     2761 attataatat caggtatgaa tgtagataga aaaggggcaa ctatctttac tcaaaaacag
     2821 agaattagta aagaatctaa atatctaata tctctgggta atgacaaaaa gtcagcatca
     2881 attagaaatt atactttggc atgtgagctt ggatatataa tatcaagtga attaagaata
     2941 ccaagtaagc aattttcaga agatgagtat gcctgtgcat ttttattgcc aaaagagagt
     3001 tttttaaaag atttgactca tccagaagat ttagattatt atgtagaact aaagaaaaaa
     3061 tggattgtac ctatatctgc tatgattttt agagcatata atctagaaaa gataaattac
     3121 aaaaaataca attatcttat gaatgaaatg gataaaaaag gctggttgat agaagaacca
     3181 ctagacaaaa tgaaaggttc aagcccaacg tatttaaaaa gagcagttga attattgata
     3241 gaaaataaaa taatgagtgt aaatagtata gtatcaagtt tagaggaatt tggaataaat
     3301 ctttatccag aagacttaga attattgatg ggattgaaaa aaggattgtt atctcaagag
     3361 gtaaataaaa aaagtaaagt tataaaattt gatggtaaaa aataggtttg tattttagta
     3421 ttaaaattaa aagcaggtac aaatgattta aacattaact tagttaaaag tcttttcaat
     3481 aagtcattta cctgctttta gtgtaagaaa gaatattgaa tctaaagtgt ttttgaaagt
     3541 gcaatatatt cccttaaaac agaacaagat ttattaaatt cgttttgctc atattcagtc
     3601 atattgattt caataatttc ttcaatacca tcttttccta taacacatgg aacacctgca
     3661 aatacatttc tttcgttata ttgaccttct agtaatgtag aaactggaag aacttttttc
     3721 tcattgtgta gtatagcttt tacaatttct actaaagcag tacctatacc aaattctgta
     3781 caatctttac catttattat acttattcct gtttttttat ttttttcaac tattgatggt
     3841 aagtctaaat tgctataagt acttgggttg tcttttatca tgtctaagat tggtttacca
     3901 ccaattgaaa catgagacca aggaaccatt tgagaatctc catgttcacc catagaataa
     3961 gcttgaattg attgttgagc gattccagtt tcttcagata aaatccttct aagtcttgtt
     4021 gaatctaaag ctgttccagt tccaatgact ttgtttttag gaaagccaga tttttcccaa
     4081 atataatttg taactacatc aacaggatta gatatgacta caaatatacc atcaaagcca
     4141 gaatcaacta tgggttttac tatagatttt atcattccaa tagttggtct tagtaaatct
     4201 aatctagttg tgttttgtct acgtaaaggc ccttctgaag agtctgcaac acttatcact
     4261 actatgtcag aatctttaca gtcagaaaaa gttccttttt ctatatgtac tttatgtggc
     4321 agatatgaaa ctgcatcagc taaatctaaa gcttgagctt ttgatttaga ttcatctata
     4381 tcaatcataa agagttcatc acaaactcct tgagttatta aactaaatcc acaatgagaa
     4441 ccaacatgcc ctgaacctac aatagatatt tttcttggct ttattgccat tttaaatcac
     4501 tccccctaaa ataacgatac attgaaaatt atagatgtta ttaaaagctg tttttgaata
     4561 ttttaatatt ctattatttc agaaaattta tttaatatga ttttatacta aaatttttaa
     4621 aaaagcaatg ttaatatact ctctattagt gaatattaaa gtattatatg tatattaata
     4681 attattaaaa tatgtaatgt cactgatagt tagaatattt aagtgttaaa agatgtgtat
     4741 aaataataat gatgtcagtt tataatgata ttaaattgag gtatactgtt tctccaaatt
     4801 aaaaaataaa ggagtaaatg gttatgaatt atcatacaaa agaagaattg gtggaagcac
     4861 tgagagtagt atcttcaagt attattaatt gtggaaaagg acagaagaaa ttttctgagg
     4921 agacatctca tcatacacgt tttaagaata ttatagaagc aatgtatatt tcaaaatcat
     4981 taataatgga tgaaattagt aaaagagatt gaagcttatt ttgcaattta acatgtttta
     5041 atatacattt tattatataa gaaaataagt aaacatacat gttataaaat attgaaataa
     5101 tatacaatta taaatttgtt aacattaata tctatatgtt aaaatatagt agaagatgtt
     5161 agtaaataat aggatgagtt ctattttaat acaattaaat taagcttagt ttgaaatttg
     5221 tatctaaaaa gtaaagtaat ctagaattaa gaaaatggag atagaattat aatggaaaaa
     5281 ttatattata caaatcagta tataagagat tttacagcag aaataattga aataaaagaa
     5341 gtagaaaata agtatcatgt tttattagat aagacagctt tttttcctgg tggaggaggt
     5401 cagtttggag acctgggaaa aatagagaat gatgatgtaa taaatgtgta tgaagatgga
     5461 aaaaatatat accatgtatt agataaaaaa cctataaaga tacataaggt aaagtgtagt
     5521 attgattggg atagaataga agatggaatg catcaacatt ttggacaaca tgtattgtct
     5581 ggatgttttt ttaaaacttt taatgcaaat acgacaggtt ttcatttggg aaatgagtat
     5641 agtacagtag atatagaagg taatttagaa gaggataaaa ttagagagat agagttgttt
     5701 gccaatgaaa ttattcgtca aaatatacaa gtagagacat tacttccaac gaaaaaagaa
     5761 ctaaaaaaga tttggttaag acgtgatttg ccaaatactg atgaagagat aagagtaata
     5821 aaaataggtg atttagatac gaatgcatgt tgtggagtgc atccaaaatc aactggtgaa
     5881 ttgagaatga taaaaataaa gaaatgggaa aaacataagt caagtacaag aatagagttt
     5941 ctagcaggta aaagggcagt ggatgaagta ttgaagaggg atatatattt gactaaaata
     6001 tgtagatatt taagctgtag tgaggaagat gctataaatg gtattgtaaa tctaaatagt
     6061 aaagtggaag aggctttaat taaaaaaaga agacttgaag aagtagtatc tacatatcag
     6121 gtcaaagaaa tgattgaaaa agcaaataag gttggcaata tttcggtcat taaaaaaatt
     6181 tatgatgatg aagatttgaa atatgtaaat aagatagcaa ataaaattgc agaatatgaa
     6241 aatagtattg cattattagc tgttaaattt aatgataaaa taaatttagt ttttgcatgt
     6301 tctaaaaatt taaaagatat taatatggga gttttattaa aggatgcaat aagtcttata
     6361 gatggcaagg gtggtggaag taaagtatta gctcaaggag gaggtaagaa taatggaaat
     6421 ttagaggcta cacttgatta tgcatttatg aagatagaaa aaactctata aatatattat
     6481 ataaaaaacc ttagtagata aaatagatat ggaggaataa gtatggagca acaaaaggac
     6541 tacatagagt tatatgaaaa tgaagaatta atagagataa gagaagcacg tatggatgat
     6601 ttggatacaa tagcaaaatt taattataat ttggctaaag aaactgaagg aaaagaactt
     6661 gatatggatg ttttaactaa aggtgtaaaa gcattgttat tagatgagag aaaaggaaaa
     6721 tatcatgtat atacagtatt tgataaagtt gttgcacaga ttatgtatac atatgagtgg
     6781 agtgattgga gaaatggaaa tttcctttgg attcagagtg tatatgtaga taaggaatat
     6841 agaagaaaag gtatctttaa ttatttattt aattatataa aaaatatatg tgataaagat
     6901 gaaaatatag ttggaatgag attatatgta gaaaaagaaa acataaatgc aaaagcaaca
     6961 tatgagtctt taaatatgta tgagtgtgat tataatatgt atgaatacga agtaatacat
     7021 tcgtaatata aatttgtaat actgttatac tgatgttata aagtattgat tatattgacg
     7081 attatatgaa ataaactgga tattataatt agaattttta gggggtataa aaatgggcgt
     7141 tttaaaagga atgttttatg tatttttatt tttagattta gtgtcaatat tctgtttctt
     7201 tttcaataaa ggaaaaatag catctaataa aattgtgttt aatgcaatag gagtacttac
     7261 atttgtactt tgttttatgc tttttagtta ttatcctaat aataatctta ttggaaaatt
     7321 tattgcttct ctatttttta tatttggagt tgcaggagtt atgttgaaag aaaaaaattt
     7381 tttatatgct agacttcttt taactgtagt tatagttttt tctacattaa gattgttcgt
     7441 tattcaataa aatgtatttg tatgaaaatt tattaaagtc tacacattta agtgtaggct
     7501 tttttgattt tttgtttgaa aattaaggaa attttaagga aattttaaaa tcgtcttaaa
     7561 tatttattga tatacttaaa atattaatag cgaaaggaga cttatcagtg atagaaagta
     7621 aaaagtcaga aaaattgtta ctgtcaatta tatacataat atttttggta tatattttaa
     7681 tgattataag aataatttta tttagagatg taccaattta cgctattttc aagggaacgt
     7741 ttaggaatgt aaatttaatt ccattttata caatatatca atttatagta gatagtaatc
     7801 ttgactttat gaaagctact gtaaatatta tagaaaatat aggtatattt attcctatgg
     7861 gaattttttt acctatagta tgtaaaaatc taaataaaaa aactataatt atagtaatta
     7921 tacttgttag cctagcattt gaattaacac aatatatttt tgctcttgga agtagtgata
     7981 tagatgatgt tattttaaat tcattaggtg gcataattgg aattaatatt tacataaaca
     8041 tgaataaatt atttcccaat gatattaaca gatttaaggc tataataggt actagtttag
     8101 ttttaggagt tataggatta ggagttattt ctaaaaatta tcataactta ctaactttta
     8161 aatttagacc tgatagaaaa atatcaaaaa tattaattga agaaaatagg gaaataatta
     8221 aagatataaa taaagataca gtagatatag ttgggacatt tgagagcttt aaaaaaggaa
     8281 taataactat taaagcaggt tctaataaca aagtaaagag accagagaat ttaattgatt
     8341 ctgatggaaa tataagaatt tatttaaatg aaaatacaaa attagtatct tatataatta
     8401 ccaaagaatc aaaaatggat atagttaaat atgaggaatt tgatgtgaag aatttagatt
     8461 tattaaggag atatgatact attaatgttt ggattgataa agaaaatcag ccaaaggata
     8521 aacatggtat tatggctagt aaattattaa ttggattgta tgaataaaat attaaacaag
     8581 aataaaagaa taacaaatat aaaaaacgaa aaaactattt ctataattat ttttacgttg
     8641 tgcataaaat aagaacatta gtgaaggagt agagataaat tatggagata ataaaatata
     8701 tcttgtactt ttttatatat tcatttttag gatggacagt agaaagtata ggatgttcta
     8761 tagcaagcaa aagaataatc aacagaggtt ttttaaatgg tccaatatgc cctgtttatg
     8821 gttttggagc ggtaattgtg atatcactat tgggaagatt taataatgta gtaatagtat
     8881 ttctattagg aatgatttta actacgatac tagaatattt tacaggattt attttagaaa
     8941 ccttatttca tgctaaatgg tgggattatt ctgacagaaa attcaatata aagggtagag
     9001 tatgtttaaa aaatgcaata tactttggtg taatgtctgt tttaataata agatttatac
     9061 atccatttat aaaatatttt gtaagtataa ttccatatag aatattaatt tcaatggcta
     9121 taattacaac actttggact atacttgatt tgattgttac tattataaca cttaaaaaat
     9181 tagatataaa gttaaactta ctagacgata taattacaga ccttaatgat ataaatgtga
     9241 aattggatga atttgataga ggagaaatac aagctttatt taaaacaata aataacgacg
     9301 aattagaagt tagagaaaaa ataaacaaaa taaatagtaa gttggaccgt atagagtcta
     9361 atattatttt acaaaaaaga gttataaaag cttttccaca tataaaacat aaaaaacaac
     9421 aagagcaact agaacatttt aaaaaaatta ttaatgagaa aaggtaatta tacaatttaa
     9481 tgaagacgaa tagaataatg gtatttataa gatgattttc agaggactct ataaaaatag
     9541 aatagagtcc tttatttttt gaaaaataat gtttatttga taaaaataaa atatattgtt
     9601 tctgaataac aaatattttg aaaattataa aaacatatat tgacaatttg tgtcaaaaat
     9661 actaacatta agtatataaa gaattttata tgagaatatc tttataaatc gcaataattt
     9721 taaaacataa atttatttaa agaggggatg atttgccaat gagcgagata actagtcaaa
     9781 tgataagtac agaagaaaaa aaatatgttg ctaaaactca aaagatacct tattatccag
     9841 tagcatttaa atcaggtgat ggtgcaatgc tatatgatta tgaaggtaat gaatatgtag
     9901 attttttagc aagtgcaggc tctgcaaatg taggtcatgg taataaggaa atctcacaag
     9961 cagttaaaga acaaatggat gatataacac aatatacact tgcatatttc catagtgatc
    10021 ctcctgtaaa attagctgag aagcttgtgg aaatagcccc tggagataat gataaaaaag
    10081 tactatacag tgcaactggt tctgcttgta tagatgctgc tataaaatta gcaagagggt
    10141 atactggaag aacaaagata atatctatgt gtgaatctta tcatggaagt acttatggag
    10201 ctatatctat atctgcttta agtacaaata tgagaagaaa aatgggtcca ttgttaccag
    10261 aagtttatca cttccattat cctgataaaa atagaacagc taaagaatgt ttagatgaga
    10321 tagaatatgc ttttgcacac tatctaccag cagaagaggt agctgcaata tttatagagc
    10381 caattgcagg agatgcagga ataatagtgc ctccagtaga gtgggtacaa ggtttaagta
    10441 aaatatgtaa agaaaatgga atattacttg taagtgatga aatacaacaa ggtatgggaa
    10501 gaacaggtaa gtggtttggt atagaaaatt ttggtgttga agcagacctt atagttcttg
    10561 gaaaatctgt tggaggtgga ttacctctag gagcagttgt tggaagaaca gaaataatgc
    10621 aaagtcttga tgcaccagca catttattta ctttagctgg aaacacaaca gtatgtgtgg
    10681 ctgctttaaa atctatagag ataattgaaa aagaaaactt acttcaaaag agtatagaaa
    10741 tgggagatta tattaaagca ggatttgaaa aactaaaaga aaaatacgat ataattggag
    10801 aaataagagg cataggatta tctataggag ttgatatagt taaaggaaaa ggttctaatg
    10861 aaaaacatcc agatgctaca gctaagatat gttatagatg tatacaaact ggattaataa
    10921 tgatattctt aggacaatct actttaagag tgcaaccacc tctagtaata actaaggagc
    10981 aagtagataa agcaatgaac atcatagatt ctgcaataga tgattattta aatggtagaa
    11041 ttggtgatga agtatatgaa gtaactcaag gttggtaaaa taaatattaa atgaaaatag
    11101 gagttatctc aattttagaa aattatttct gaaatgagat aactctttta agttacccac
    11161 aaataagaaa attgtttgat tcattaaaga ctatatttcg gatgtcgttt ttgtgagtat
    11221 cagagacagt attacgagaa taattacctt tattcgtact atctgttaat ttagttataa
    11281 ttaagatagc taaaagaata agaattactg tgtataaagc aataacatat ggttttcttt
    11341 tatatttgta tttgttattg aaaaaagaat gaccataatt atttttattt actttagatg
    11401 tatttgtatt ctttttcttg ttacctttct ttttatcatc cttttttcta tttttagggt
    11461 aaggaatgac tttgtgtgta ttatcaagtt tgatattttt atttttttga atattgtttt
    11521 ccaggctagc ctttttttct ccaaattgaa tagttttatt ggtattgttg ttatctttca
    11581 atgttaatct ccctccctta aactttccct ttagataata ttactactaa cctatggaat
    11641 atacaataaa taatatataa gtaataaaat tttataggta taaatctctt tctccacgct
    11701 ctaaggcagt catattgtta gatactatgt ttcttatatc ttctctttct atcttaccta
    11761 tttcttctgc gataagtttt ttacccataa ttttaagttc ttcatctcca taatcgatta
    11821 aaaattcatt taatgtagta agagcatttg gtgtacatac attatggatt tgtcgacttt
    11881 tagctaagtt catgaatcta tcgccagttc tacctttacg ataacaagct gtacaataac
    11941 taggtatata tccatcagta atcagttctt taagcacctc aacagggctt ctatggtctc
    12001 caacttcgaa ttgttctgta ttgtcaccat cttcatatgc tttatatcct ccaacacctg
    12061 ttagagaacc tgcactaatt tgtgatacac catattttag taactcattt ctcatttcag
    12121 cagtttctct agtggacatt atgattccag taaaaggaac agctaatcta gttatagcta
    12181 ctattttttt gaacatatcg tcagaaacta agtgaggaaa atcttctaag ctcatacctt
    12241 cagctttttt caatctaggg aaagatatag tatggaaacc tacaccaaat ttttcctcta
    12301 agtgttcgtt gtgcatcata agtcctagaa cttcaaattt agggtcagat aaaccaaata
    12361 aaactcctgc accaacatca tcaacaccag cttccatagc tctatcaaaa gctgttaaat
    12421 ggtaataata gtcatttttt atagattgac catgcatttt tataaatgtt ggtttgtgat
    12481 aagtttcttg gaataatata taagttccta ttcctttttc ttttagtaat ttatactcat
    12541 ctacacttgt agctgctatg ttaacgttta cccttcttat atttccgttt tcattatatg
    12601 ttgaataaat tgcatctaag caatctaaga tgtaatttat atcacaattt ttagggtctt
    12661 caccagcttc tagagcaagt cttttatgac ccattttttc taatatttta acctcttccc
    12721 ttatctcttc catggtaagt ttttttcttt taaatttgtt gcatttacta aaaccgcaat
    12781 aaacacattc atttacacag tagtttgata cataaagagg ggcaaataaa accactctat
    12841 taccatatat ttcatttttt atttgcccag ctatactaaa taatctatct agttgttttt
    12901 tatcttctac ttcaagaagt gtagctatat ctttataact aagtttttcc cttctatcag
    12961 ccttatcaag taccttttca atatcatcag aagttgaatt ttttgcatct tctaataaag
    13021 aattaataag ttcatggttt atgaacattt acaaacgcct ccattcaata ttatcaccgc
    13081 gagaatagtc aacttcaagt cctaatgact tgatgttttc ttctaaactt tggtgatatt
    13141 cacaagcttc ttgaccagta aaggctttgt tatcgtaaag agaatattgt tttctaaatt
    13201 cttgaggaga aaggtttggc ataattacat tgcaaccagc taaaagacct gcatttctac
    13261 cactagggtt tatagatgcc aaggctgttg tagcgggtaa taaaactttt ggaagtagaa
    13321 gtcttgtaat tgaaagcata agtaatgtct tctctaaatc tccatgttta taatccttaa
    13381 aaattgtatc atggtgtgga ataaaaggtc ctattccaac catatgagga tttaactctt
    13441 ttaggtataa taaatcacga acaaggtctt cgttgttctg aaatggtgat tcaaccataa
    13501 atccagcacc tacctgataa ccaatttctt taaggttttt taggcattct tttctagttc
    13561 taagctcaat gtttggcgga tgtaattttt tataatgact gtctgtagca gtttcatgtc
    13621 ttaaaagata tctatctgca ccacataaaa agtatttttt atacgaatca taactctttt
    13681 ctcctagtga tagagttatg gcacaatcct catatttatt ttttatactt gaaacaattt
    13741 cacatatttt atcatcagta aaatatgcat cttcaccacc ttgcaaaacg aatgttctgt
    13801 atcctatttt ataacctata tcacaacatt caagtatttc atcaagtgaa agcctgtatc
    13861 ttatgatatt tttattagaa gattgtattc cacaatagta gcaattattt ttacaatagt
    13921 ttgtaagctc tataagtcct ctcaagtaga ctctatttcc ataatgttta tttctcatgt
    13981 cgctagcttg tttataaaga tagtttctta aattttgttc gaagttatat tcctcattgt
    14041 cgatattttc tattaaatat agtagttcat caaaacttaa tgaattaatg ctgtaaagtt
    14101 tatctataaa gtatttaatc ttatttttag tcatattcaa atatgtaatt attaagtata
    14161 gttaaatttt taataattga aaagttcaag tgatctttcc aaaatacctt ttacatatgc
    14221 tattaagaca ccatagttta caataggtac attgaaagat tttgcctttt ctattctaga
    14281 taacattcca gctctattca tcatacaagc tccacagtga actacaaggg catatttatt
    14341 tatatcttca gtgaaggata ctccagaact aaattcaaaa gtaatgtttt tgccagtttt
    14401 ctttctaatc atattaggaa tttttactgt tccaatatca tcagtttgtc tatgatgggt
    14461 acatccttcg gccatcaaga ttttatcacc atcttttaaa ttttctaaag cataagctcc
    14521 attaatcaat tctttaaggt ctcccttttg tctagcaaac aatatagaaa aagatgtaag
    14581 aggaatatct tttggtgtat ctttatctac ttgtggaaat acttgagagt cagtaataac
    14641 aagttttggt tttttgccta aattagatag agtttctttt aaactatctt cttttgtaac
    14701 tatagaaatt gcaccattat ccaaaatatc tcttataact tgctgttgag gaagtataag
    14761 tctacctttt ggagctgctt tatctattgg aacaactaaa acaactaaat catttggttc
    14821 aattaaatct gataccaatt tgaactcagt gctatcctta ggcaaaactt ttattatttc
    14881 attttttaaa ttttcaattc caattttatc tgttgatgag acagaaacaa ctggacattt
    14941 aactttattt ttagttaagt ttaaaatttc tgattgattc ttaatagtat caattttgtt
    15001 aagaattaga atatgaggga tattcttatc attaaatttt tcaattagtg acaaatcttc
    15061 ttgtgatata ccaatttggc aatccactac tagtaaagct atatctgtct tttctaaaac
    15121 atctagtgat ttacctattc taagttcacc aagttcacca acatcatcaa gaccagcagt
    15181 atctataaga acacaaggtc ctataggaag gatttccatt ggtctaaata ctgggtctgt
    15241 agtagttcca gcaatatctg agactattgc agcactttga tttgttatag catttattat
    15301 gctagattta cctgcatttc ttttaccaaa gagacctata tgtactctta ccgattgtgg
    15361 agtagaattt agactcattt gtactcacca ccttaaactt ctttttttga tatactagtt
    15421 tttacagata catgaggtat gttgcccaat ttaccagtca aactattaat agtatccatt
    15481 tcgccaacaa ctgctataca tacgattgag acaccttctt cttcgcaagg aatgcccatt
    15541 cttcctttaa taataccttt aaaacctgat acaacctcat tgaatttaaa ttgacattct
    15601 tttggctctt ccaaaatagc acttataact gcaacctttt tcataaatat cctcctaact
    15661 cagaaaatat gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa agaaaagttt
    15721 ctacaaaaga aaagttatta attacacaag tataatgtgc tatattaaac tatttcctta
    15781 cagattagat aactcttttc tggtagggtt agcttgtaaa aatttaattt aacaacaagt
    15841 aactgaagaa aatttttata caatttatat aaattataat aaaaattaac taaagaaaat
    15901 tgaagttact tatataatag tagtaaattg aaattaagtc tatagaaaaa ccaatttttg
    15961 tataaaaata aaaattaaag ttaattattt gttaagaatt gactttggta aaatatataa
    16021 ttaattattc tttaagcata taatttataa gtaattttag tgtattttca atagcttcta
    16081 tatgtgttct ttcataatgg tgactattgt ttgttccagg accaacacat gctatttgaa
    16141 catcttctcc ccaacgaata gcttctgaag catctgaacc atagaaagta aatacatcga
    16201 cagcgtagtc taaattattg tcttctgcaa catcgacaag cttgtttctt aaattaaaat
    16261 catataatga taaattatct tttgcaacta tagttacacc atgttcacta gatgtttgac
    16321 ttggtccagt aggtgctata tctattgcta caaattcttt tgttttttca ggtaaacatt
    16381 tagaaacacc atgtccaatt tcttcataat tacttatata aaagttagta gtatgcttag
    16441 gtgttaaatt gttttcttta aaatatctac atatttcaag aaccattgct acagctgatt
    16501 tattatctat atatctagat tttataaaac cagattctgt agcctcaaat ctatggtcta
    16561 ggcatacaaa atccccaaca cttattccta aatctaatac atcttgcttt gtaaatactt
    16621 tttcatcaag tctaataatc attgtttctt catttctttc agtggcagct tttgtttgac
    16681 catatatatg agtagaagca tacttaaata caacacttcc tctgattttt ttaccttttc
    16741 tagttataac agtacagttt tctccctcta tagctgccca acatccacca ccaactttat
    16801 ggtatttaag agtaccatca gatgatattt cttttactat agctccaagt gtatccatat
    16861 gtgcagatat tgttatttga tttgaatcat cttcaccagg aagagtagct ataagtgcac
    16921 ctttttttgt aagaatagtg tcaagaccta aattttcaaa atcttttttg atttctaaaa
    16981 ctgcattttc agtatatcca gctgggcttg gaatgtccaa atattttttc attaaattta
    17041 tagtattagt taaatttaaa ttcatattgt ttcctcctaa ataaatatat ttatacaaat
    17101 tataacataa atacaaatgt tctgaaaact aaaattaaaa gaatggaatc taaacatatt
    17161 taaaaaaaaa gtatataaaa taaatttgat ttatattata tagatttaaa gtaaaaatag
    17221 ttttatttaa taattaataa aaataaatac aaaacaaaat aaataaataa taaaatattt
    17281 agtttaatta tctttacaaa taaaaatttt agtaataaaa taattgttga aaaagatttt
    17341 aaattcagat tattcatttc taaagtaatt aatgtgcaaa tctttattaa tatatgtcaa
    17401 tagttaggta tatatgtatt taattagcat gatatagata aaagtattta ttttaattgt
    17461 ctattatctg aaaaagtctt tacttagaaa tcataaaaat caaattaatt aggaaaagaa
    17521 agggtgtgta taatgtgatt gagactggat ttgtatattg tagtttcttg gttgtattcg
    17581 taggtgtaat tttttttatg gttgataaat tgaaatggaa ggtatttgat gttattaatc
    17641 caatgctttt aatatactta ggttcagcta tacttggatc atttggttta tttgcacaga
    17701 atgaagatgt tggaacgtat taaaacataa taacagtgaa cttccttcca ttaatgttag
    17761 tgtttttgtt aattcaatgt gatttaagaa aaatattaaa gatggggcct aaaatgttat
    17821 tatcattttt ctgtgctaca tttacattta tgcttggatt tgttatagca ttcataattt
    17881 ttaaaaattc aatgggtgca gattcatgga aaattcttgg ggcagcatct ggttcatgga
    17941 taggtggctc atcaaatatg attgcagcag ctaatgctct aggagttgca gaagaaggac
    18001 ttagttatgc aatacttatg ggttctatag gatatacagt atggatgtct gtttgtctaa
    18061 tgtctgtgaa atttggacct aaatttaaca aatggacaaa gtgcgataca agttttatag
    18121 atgaggttac atcaaaactt gaaaaagaaa ataaagataa tacggcacct acgtttgtag
    18181 aacttatgat tttactttct gttgggtttg gagttgcgtc ttttgcacaa attgtatcca
    18241 agatacttcc aacttcaggt atacttaatg aaactatgtg ggtagtactt gttgcatcta
    18301 cagtatcaat attattgggt atgacaaaaa tatcaaagct taaagggagt acactggtag
    18361 gaaatgtgtt tatgtattta ctccttgcag ctacaggtag taaagcaaat ttctttggat
    18421 tgtcagaagc acctgtttac ataatgtttg gattggtagt agtccttgta catgctattt
    18481 catttatagg gcttgctaaa ctatttaaac ttgatttatt tacttgtgaa gtaggaagta
    18541 tagctaatat aggaggagta actacagctc caatgattgc tggggttcac aatcctgtat
    18601 taataccagt aggtgtactt atggggcttc taggaaatat tatagggact tattgtgctc
    18661 ttatggtaac tcaaatatta catatgttag cttaatgaaa atatggtata aaattaataa
    18721 tttaaataag aaaagtcatt tcaatttaag cagaagcttt tactgaaatg gctttttttg
    18781 tttgacaaga taatctattt caagaagatg ttttgacata tttagtgtat aatattgtaa
    18841 aatttactaa aaacgagata tatttaattt ttatattgaa tcgaacgtct gtatgatgta
    18901 taataaatgt acagaacgaa tgtttgcaga acgggtgttt tttgaattta ttttaataat
    18961 ttatgaatat atatcagaat gcataaatat atttaaatat aaagattaaa aagaatttaa
    19021 aggagtgttg ataacttatg ataattccat caagaagaat acaagatata acattaacaa
    19081 atttaaaaaa tggtgaagta actttaattg aattggatga aatatacaag aagatgggat
    19141 ttttatttgt tgttagtgaa ggcaaattga aaaagataaa aaaggagaat catcattaga
    19201 aatgaatgaa ataataaata ttataatagt ttataaaacc taacttatgt agaatataaa
    19261 tacttaagtt aggttttttt gtgataaagt ttataagtac tcaattttat aagataaata
    19321 atggttttaa gtgaaaagta aataagttta aattttagta agtattatat tgcataagag
    19381 gtgatattat tatgaaaata gtaacctaca atattcataa aggtatggat tcaaataata
    19441 gattaactct aactaaaatg gggttatatt taaaacaatt ggattgtgat gtaatttgtt
    19501 tgcaagaagt atcatatcct caatttttag ctttaaaagc tgttttaaat atggatggtg
    19561 tctttgctac aaatgtaaaa aaagtaaata tgatatatgg tatttgtaca tttacaacat
    19621 ctaaaatgtt aaacagtaat cattttttct taactagcaa aaaagaacaa cggggagctc
    19681 tttgtataac tatagatgca tatggaagaa ttataaatgt aataaatact cacttgggac
    19741 ttgataggca ggagagagca aaacaattag acgaaataat agactatagg aacagattag
    19801 tggggatagt tattttatgt ggtgatttta atgagaaaaa tgtcttttta agtatgttta
    19861 atgatatggc tatttcacta aacaagtctt acttaccaac atttgaaaaa tcaaactcta
    19921 gaatagatta catttttgtt aataaaaata ctgaactaaa aggatacaca gtagaaaaaa
    19981 tttacctgtc agaccattat cctgtaatag gttacattta aatttcaata taaatgaaat
    20041 tagttgttat aaaataaaca attttttgtt aagaaatagt aaaagaaata taataaaaaa
    20101 tatcaaaaat aaaactaatt ataagacata aatttacaat aaagttacat ttgattaaat
    20161 tccttgttta acaaaagcta ttcaaatata atctaaggta gttttgaaat gggaactcta
    20221 ttatgcaaat ttaagctagt gttaaaatga ggtgaagtta tgggcagaag aacaacaaga
    20281 gtttcaagat taggtaaaga aaaaaaacga agtgttttaa ttgtattggc agctttagtt
    20341 ctaataatgg gtggatttac ttattttttt aacagtaaat ttctgtacaa tgggaaaatc
    20401 gctaaaaatg tatatataga aggagtaaat gtatctgata tgactaaagc agaggcatta
    20461 aaagctataa cagataagta tactccagaa gatttaaatc ttgcttatga tgggaaaaaa
    20521 tatacaatat caccaaaaga tattgattta aaatatgata cagaagatgt tgtaaaagat
    20581 gcttatgaaa gtacaaaaaa agggtcctat tttcaaaact taaaaaaata tattgatata
    20641 agagtgaata aggcaaatat gaagataaaa gctgaatatg atgaggcaaa acttagttcc
    20701 aaagtgtctt ctatagcaga tagtataaat gttaagatga aaaatgcttc tattagtgtt
    20761 ggaagtggtg gactaagtta tacagactct gttgtaggta gagaatttga tttggcagca
    20821 aataaagagt ctatatataa tatgataaaa aataaagagc ataaaacttt agaacttaaa
    20881 gtaaatcttc aaaaaccaga tataacaact gaacaagtaa aaactgtaaa ttctgttata
    20941 ggtcaatatt caactactta ttcacaagct gtagagggta ggtcatataa tgtaggatta
    21001 tcagcaagaa aaactagtga tgtattatta atgccaggtg aagaattttc atacaataaa
    21061 cttacaggac ctagtaataa agcaaatggt tacaaagatg cacctgttat agtttatggt
    21121 aagctagaac aatcagcagg aggaggtgtt tgtcaaacat cttcaacagt ttataatgcg
    21181 gctcttcttt cgggtatgga gataactcaa gttacaaatc attcatctgc atctacttat
    21241 gtgcctaaag ggagagatgc tactgtgagc gatggaggat taaacttaaa gtttaaaaat
    21301 ccatataagc atcctgtata tattaaaaat tatgcaggtg gaggaagtgt atcatcagta
    21361 atctatggta attctggtga caaaccaaat atatctatag aggtaaaaca aactggtcag
    21421 aataaatatt ctacatatcg aatatttaaa gattcaagtg gaaaggtcat aaaaaaagaa
    21481 catatatcaa atagttctta taaagaatta aaaaaataga taatttttta gagaaatatt
    21541 tgtaatataa aagggagtgt ctcaaaatga actttttagt ttatgagaca ctctttttct
    21601 gtgaaatcaa atatattttc atcatttcaa caatacaaaa gaagcgtatc tctattttga
    21661 gacagccttt tttgtaaaaa ttaacactat tagttcttgt tttaatatta caatcattaa
    21721 tatatagaaa atattcctat atattggtat aattgtaaat agatttagtt atataaaatg
    21781 tatgaggagg tgacatggtt gaattttata ggcaatagat atgaggtagt aaattctgac
    21841 gacgttttag aaattaacaa aatctataaa gctagagatg ttttttacag gaaaaatgtg
    21901 ttaataaaag ttataaagca caataattat atctgtgaag attttgtttc aaatttaatt
    21961 gatgaaagta ctgctttaga tgagataaac tctccttata tactaaaaat aatagatgtt
    22021 gggattcact gtacagaaga aacaacatta tattatgttg taagtgaaga ctttaatggt
    22081 atcggactag atgaattaat tctaggtaat tatcttcatt tagaagcaat tgtaaacata
    22141 atgatacaag ttttaaaagc tttagaaatg atacatagga atcattcata tcatggtagt
    22201 ttaaaatcga gtagtataat agtagataca gaatacaata taaaaatttg tgattttgga
    22261 attacaaaag caaataatgg ggttaataca agaagctatg gaaacaggag atatttatgc
    22321 ccacatcaac tatgtataaa ttatactgat aaagaaagtg atttttttgc aacaggtctt
    22381 attctatttg aatctatatt taaaaaattg ccttttggag aatcaaattc agaagaaaaa
    22441 atgctacaat ctattgataa gggattagat tggaatagta taaaagcaat taatggaaat
    22501 actgaactta taaatgttat aaaaaggctt cttggaagaa ataataagta tactagagcc
    22561 aatgatatta ttattgattt aagtaaagta atgtatgaaa aagcatatat agaagttgaa
    22621 gatgagaaag aagaagagca gaaacaagac aaagttatac aagttaaaaa agaatataaa
    22681 agtaaaatga gaaaaaaatt acataaaaaa cttgttgttg ccggtctagc tatagtcatg
    22741 atttcaatga ttattattag ttccataata taaaaattgt atattaagta ttttaaaatc
    22801 aatgtctata aaggatatac atagaaattt tagtgtagtc ttaaattatg aaaggaagtg
    22861 gttattatgt taacaaaaaa taatgaggta ttaaacgtct aggagtattt taacaaatag
    22921 ccacctgctt ttacagttta gaataaaaaa gctgtggcta aaggtcacag ctttttgcta
    22981 ctttttaaag gcaggagaat attaaaaagg agcaaattat gagcaatata aaaataatag
    23041 caagtaataa atgttggatt gaagatatag caaggaaaca acttgaagat atttcaaagt
    23101 taaatggaat tttaagagta gtaggattac ctgacttaca tgcaggtaaa atacctgtag
    23161 gtttagcagt tgagactaaa aatattattt atccacatat aattggaaat gatataggat
    23221 gcggaatgac tttatttaaa actggggttc taaaaaagaa atttaaaaaa gacaaatgga
    23281 ttaaatcact tagtaagatt aaagatttgt ctgatataga aataaaaaac acatataaag
    23341 aagaatgtcc aattttaaat ttaggaacta tagggagtgg aaatcatttt gtagaaattc
    23401 aatgtataag tgaaatttat aataaagaac aatttgaaca attaaaattt tcatctgatg
    23461 atattatgat gttagttcac tgtgggtcaa gaaattatgg agaagatatt ttaaaaaaat
    23521 tttatgataa agatggactt gaagttgaaa gtgagaaagc tatagattat attaaaaaac
    23581 atgatagtgc tttaatgtgg gcagagcgta atcgtgaagc tatcagtaaa aaaattatgc
    23641 agtcaatagg tacatctgga gatgttgaaa caatattctc tataaatcat aattttatag
    23701 agaaaaggga agataaattt atacatagaa aaggtgctgt ttcaagtgaa agaggagctg
    23761 tgattatacc aggttcaaga ggcagtctat catatatagt tatgcctact gaaaatacag
    23821 agatatcttt atattctcta tcacatggag caggtagaaa atggtctcgt tcagtttgta
    23881 aatctcgcct taaaagtaag tatagtaaag atactataaa acaaactaaa tttaaaagtc
    23941 agattatatg taatgatgta aatttattat ttcaagaagc tccagaggca tataagaata
    24001 tagagcagat tattgaaagt ttgatagaat atgaacttat tcaagtagtg gcaactatga
    24061 agccattgat tacttataaa ggataatttt taaagttaaa aaagattaaa attatattat
    24121 aaatagctaa ttttatagaa catttaaaag tgttttatta ttagctattt ttttattttg
    24181 tagagacgat aatttataga gctaaattct ttgatatata atgattacaa agttagactg
    24241 tattggaaaa tgcacataca tgaattgatg acaatataag tgattcccaa atataaatga
    24301 aagtataagc ttattttcac tataaaaatt ttcactgtac aaaaacatta atgacattat
    24361 ttttattgac ataaatatat aatttatgga ataatattag aagcactgat ataagtgttt
    24421 aaataagaat agaaaaggag aagtgtttta tgaaaaaaaa tactaacatt aatgagtccg
    24481 gagttaaact tcttaataaa taaggagttt gatatataat aactccttat ttattaagtt
    24541 gttcgcaaaa taactattat caaagtaaat gatagggtga tttaaaagac gataacttat
    24601 aaataaagga gaaaaaataa tgaatagtaa tacatttgaa agattacaac taaatgaagt
    24661 taaagaatta ataaaaatac attgtgtaag ttcactagga aaaaatttaa tagataaatt
    24721 aactcctagt gggaatattg gagtagtaag aaggaaatta gcagaaaata aagaagctag
    24781 aaagataatt gaaaatagta atcacatacc acttgaagga ttgtttaatg caggttctac
    24841 aatagataaa attgaaaaag gaatgataat agaacctgtt gaacttgtaa atattgaaga
    24901 ttttttaaga ggttgtagga agatgaaagc ttttatgtta gaaaaagaat tttattcacc
    24961 tacattaagt tcttatgcct taaatataac agaatgtaaa agtatagaag atgagataaa
    25021 ttactgtata aaatctaata aagttgattc taatgctagt aaagaattaa aaaagataag
    25081 acgaaatata gaaattacag aaggaaagat aaaagataga ttaaataaat ttataacatc
    25141 aactgtaaat aaaaagtata tacaagagtt tataataagt aaaagaaatg atagatatgt
    25201 aataccaatt aaatcttctt ataaaaatga agttaatggt actatattag atacatcatc
    25261 aaaaggaaat actgtattta ttgaacctat tagtgtttca aatttaagta cagagttaac
    25321 tatgctaaaa gctgatgaaa ctattgaaga atacaaaata ttatcttatt taacagaact
    25381 tatatttgag aagataagtc agataaaatt aaatatagag atactttcag aatacgatat
    25441 ggtatttgca aaagctaagt atagtcaaaa aataaaaggc attactccaa aaataaataa
    25501 taatggctat ataaaaataa ttaaaggtaa acatccactt ctaacaggag atgcagtacc
    25561 tttagatttt gaaataggca aaaattatag aagtcttata ataacaggtc ctaatgcagg
    25621 aggaaaaact gtaaccctta agactgttgg cttactaact ttaatggttc aatgtggatt
    25681 tgatatttct gcaaaagaag gtagtgaatt tagtgtattt gaaaaagttt ttgtagatat
    25741 aggggataat caaagtattg aaaatgcctt aagtactttt tcatcacata taaaaaatat
    25801 tgcagagatt atgagtttat caaataattc tactcttgtt ctgtttgatg aaataggaag
    25861 tggaactgaa cctaatgaag gagctggttt agcaatatca ttattagaag agttttacaa
    25921 aatgggatgt ataactatag catcaactca ttatggagag ataaaaaagt ttgctacatt
    25981 gcatccagaa tttgaaaatg ctggtatgat gtttgataaa gaaacgttag aaccattgta
    26041 taagttaact attggaaaat ctgaagatag taatgctctt tttatttcta aaaaaatggg
    26101 aataaaaaat agagttttag atagagctaa agaatatgta attgatagaa actacaattt
    26161 agatttagtt aaaagaagta agctacaatc taatattgaa gataaaaata gttctgaagt
    26221 cattttaaac tatacagatt ataatgtagg ggataaggta aaattactag atgaagatga
    26281 ttttggaatt gtatataagc ctatggacaa gtttaataat gtagaagtat tgtttaatga
    26341 taattttatt tcagtaaata taaaaagatt acaacttagc ataaaagcat cagaattgta
    26401 tccagaaggg tatgatttag atgtattatt tactagtttt aaagaaagaa aattagaaaa
    26461 agatattcaa agaggttcaa aaaaagctct taaaaagatt cagaaagaaa ttagagagaa
    26521 tagaaaataa atataaaaag cacaaaagaa agcaatctaa agagaaaagt ttatctttag
    26581 attgctttct tattaacata tcttcataaa tccaatgact aacaagatta aaccacttaa
    26641 ccaagataag tttaaattta cgtttttaac aaacatattt cctagtaaac aaccaagtaa
    26701 tactgcaatt atatttgata ttaaagaaaa tagaataact tgtatataat tcacacctac
    26761 aaggccacta ccaaaaccga tagctaatcc atctaaactt aaagcaagtg caagtgtaaa
    26821 tgcttcagca gatttaagca ctttagattt acttgtatct aagacatcag gttcaatact
    26881 tacattaaaa tttaatttaa ggtctaacat attaaaatta taattctttg agtgtataga
    26941 ttttttctta aaataagatt taaaaaaact ttcaattagt ctagcacttc ctaataaaaa
    27001 taaaataaag aaacatatta acattgttat attttgaggt aaaaaatctc taactattgt
    27061 acctgcaaaa agcgatattg ctaaaatagc tgaacaaact acgctgataa ttatatttga
    27121 atataaggga actttaactt tatttacacc atatccaaag cttgtcacaa aggcatctat
    27181 acaaagagaa gtaactaata aaattgattc aatcacagta gattaacatc ctttctctta
    27241 aattttgtac ttatattagt tatagtattg taaaaatgga taaattgtta cagataatcc
    27301 aataaattgt tgataaaatt ccaacatcat acaacaccta aataacaaaa gaatcatatc
    27361 atataataat ataaaaccaa tcataataaa gcgggtgaaa ataatggaac aaaataataa
    27421 ggttgggaat ttcggtgatt tgttgaagat tttattagat gaaaaagaat tatcaatggg
    27481 agaactaagt aaaaaaagtg gaattgataa atctactata tctcgtatag ctaataataa
    27541 acaaaagcca aatataaatc atttagaaaa gatggcaata catttaaata ttaacctaga
    27601 agaactgttg aaagcttcag gatatgaatt tagaaacagc aataatcaaa tatttaatgc
    27661 agatagtgat tttagtaact ttgatgatat tcttggattt gcaaatttaa tcaatgataa
    27721 gaactttaat ggaaatattg aaaaagagtt aagtaagtgt aagttatatg tccaaacaga
    27781 tgaaggtaaa aaattacttt ttgataattt taataaaaaa atagattcta ttgaaaaaca
    27841 aggtcaattt acagatagat taagacaaat gtatgctgat ttttgtacag atggacttac
    27901 aataaagaaa tatttattga ttggaagtat gctactttac tttgttatat caacagatgt
    27961 gattccagac ttcgtgttcc caataggatt tatggatgat ttagtagcat taaatatagt
    28021 tacaaaatta cttaaaaatg ataagtaagt tttagaatca tatttaatat ataaataaat
    28081 caaagtatat aaaatttatt attttttata tgaaacaaat taaaagcaca atgaagtaga
    28141 ttaaaaattt aaaactaaga atctaatata tattttgtaa ctatttaagt atataaaata
    28201 aaataatatc caaactaata caaaatagta ataattgttt tgtactggag gatatatatt
    28261 tatgaattgt aaatggatat ctaaaataga tgagagaaaa aaatgtcata gagaagcaga
    28321 ttcttctgga tattgtatct ttcataaaga aaataagtct gatgaagaaa tacagttgat
    28381 gatggatact attcataaag aagagataag tgaatttaat ggatttgttt ttgaaaatga
    28441 atttaatgcg gaagaaatat taacttacaa ttataaaata ctggattttt ctgaatccat
    28501 atttaaacaa aaagcaaatt ttaaaaaata tatttttaaa aagaatatta tttttaacta
    28561 tacagaattt agagataaag ttttatttaa tggttgtgta ttcttagaaa actgtgattt
    28621 taatagaacc atatttagta aaaactatat aaatgaccgt atatttgaaa aagtgaaatt
    28681 taaaggacct gatttggtag ttaataaagt agaaaatttt ccacgaatgg atggaataat
    28741 ttttagcatg tgtacaaaat ttgtattaaa gaatgttgaa tatggaaaaa gtgagtatga
    28801 gcatggcaag ataaattata gaatagctag aaatcaagca acaaaaattg gagaatatga
    28861 gatgatagga ttttactatt ataaggaaag aatttatagt agtaaaataa tgaaacgttc
    28921 taattatcct actttcagtg attatttagt tgaaaaattt tttgaccaaa tagcacgtta
    28981 cacaacaggt tatggagaaa agccatggaa tatattatta gttattatag caataataag
    29041 tgtttttgct ttattatact tatttgtagg catagaaagt tcaaatagca cacttgtagc
    29101 tttagatata aataatatag gtgattactc actaagtgag attttcaaaa tgtatatgga
    29161 tttatggtat tttagcatgg ctacatttag tacagtagga tatggagata tggttgctac
    29221 aagtttgatt gggaaagcat tggcaggtat agaagtattt tttggagtta caataggagc
    29281 aatttgggca tctgtgatta ttaaaagaat gataaggtaa tatttgtgta aatatggata
    29341 ggtattaatt actagatata aattggacta aatagggaaa atttaaatat aaatatactt
    29401 aataatccaa gaaggtgaaa aatttgaaaa atgcactaaa aaaaattata tcattagcca
    29461 tgattctagt ttttataata ccaaattatt catatgcaga tgataaatct gtagacaagt
    29521 attcaaaaac atctatactt atagaccaag aaacaggtag agttttatat agtaaagaac
    29581 ctgatatgaa ggtaccatta gctagtttaa gtaagatgat gactttttta cttgcaatag
    29641 aagctattga aaataaagaa gtaaaagaaa ctgataaagt aaaaatagac aaatctattg
    29701 catcagtaaa agggtcaagt tataaactta aagatggtga agaagtgcca cttatagagc
    29761 ttatgagagg tcttatgatt gtatcaggaa atgatgcggc aattgctata gcaaaacatg
    29821 tttgtaaaac gaaagaagca tttgtagata gaatgaataa aaaagcaaaa gaaataggaa
    29881 tgagtaatac acattttcta aatccaaatg gtcttccaat atacgattta agcaacccca
    29941 aaaaaccagc aaaagaaaat atctctactg caaaagatat agctattctt ggaaaataca
    30001 tgtttgacca ttatgaaaaa caagtaacag ctataactga tatggagaca tatacaaatt
    30061 cagatagaag ttttgaaaaa agcaatacaa atgcattatt aagaattata ccagaagtag
    30121 atggtataaa aactggatat actggcaatg ctggatattg cttatcattt tctatggttg
    30181 taaataaaaa tgataaaaat gagaaaaaac gtagagtaat aggggttgta ttaggtacta
    30241 accataaaaa taaaagaacc tctgcttcat tagctctact gaaatatgga aaagaaaatt
    30301 tcaatatgaa aaaagtaatt gataaagata gctttattgg taagaaatat ataaaaggaa
    30361 tgaaagagtt agaggttaca ttaaaagcaa aagatgaact ttatacaata ttaaaagatg
    30421 atgaaagtat aaagtcagaa gttaagatta aaaatataga gtaccctgtt aaaaaaggtg
    30481 atactatggg tattataaaa tattatacta attcaggtga cttacttgga agtgtagata
    30541 tagtaagtaa taatagtata gataacgtat cattaaaaac aaaattaaaa atgaaatttg
    30601 caaattaaat tcaaaaaatg gtataaaata cacaaaaaaa tataaaaata gaaaatatgg
    30661 aaaaaatatt gttatttctt cacctctgtg atatactact agaggtgaaa aaataaaata
    30721 aatcacacac agcattggga tccttaaaag gtgcctatta catggttttt tagggagaag
    30781 atcaagctgg aggtataaaa ccaggaggta agatatgtca gtaatatcaa tgaaacaatt
    30841 attagaagca ggtgttcact ttggtcacca aacaagaaga tggaacccta aaatggctaa
    30901 gtatatattc acagagagaa atggaatata tataatcgac ttacaaaaaa cagttaaaaa
    30961 agttgaagaa gcttacaagt tcactaaaga agtagctgaa acaggaaagc caatcttatt
    31021 cgtaggaact aaaaaacaag ctcaagatgc gataaaagat gaagctgaaa gatgtggaat
    31081 gtactttgtt aatgaaagat ggttaggtgg aatgttaaca aaccacaaaa ctataaaaac
    31141 aagaataaat aaattaagag aattagaaaa aatggaagaa gaaggcgtat ttaatgttct
    31201 tcctaaaaaa gaagttataa aattaagagc tgaaaaagaa aaactagaaa aatacttagg
    31261 tggaataaaa gacatgcctg aattaccagg tgcaatgttc gtagttgacc caagaaaaga
    31321 aaacatagct attcaagaag ctcatagatt aggtatacca gtagttggta tagttgatac
    31381 taactgtgac ccagaacaat tagactttgc tataccagga aatgatgacg ctataagagc
    31441 cgtaaaatta ataactggtg ctatggcaac agctgtaatt gaaggtagac aaggtgctga
    31501 ggaagaagta gctgaagacc aagaataata atttgacaat taaatggtaa gggtcgccct
    31561 taccatttat aatatggaat agaataagta ggaggaaact taaaaaatgg ctaatataac
    31621 tgctcaaatg gtaaaagagt taagagaaag tacaggcgca ggaatgatgg actgcaagaa
    31681 agctttacaa gaagctgaag gaaacatgga aaaagcagta gatttattaa gagaaaaagg
    31741 attatcaaaa gctgctaaaa aagctggtag agttgctgct gaaggtctag ttgcaataga
    31801 aatgaatgat gataatacag ttgcttctat ggtagaagtt aactcagaga cagatttcgt
    31861 tgctaaaaac gaagacttca aagtgttcgt taaagatgct gcttgtatgg cattagctac
    31921 tgataaagaa gatatagctt ctttattagg tgaaactcat aaagagggaa taactttaca
    31981 agaagtttta aataatagag ttgcaaaaat aggtgagaaa ttagacttta gaagatttgc
    32041 taaagttgta actaatggac aagttgctgg atacattcac ggtggtggaa aaataggtgt
    32101 tcttgttgaa atggaaacag aagctagaga tgctaaagtt ttagaattag gtaaagatgt
    32161 agctatgcaa gttgctgcta tgaatcctaa gtatgtttca agagatgaag tagatgctga
    32221 atacatagca catgaaacag aagtattaac tcaacaagct ttaaatgaag gaaaaccagc
    32281 taatatagtt gaaaaaatgg ttaaaggaag attagaaaaa gaattaaaag aggtttgttt
    32341 attagagcaa actttcgtta aaaatccaga tataactgtt aaacaattag ttgctgatgt
    32401 tgctaaggca gtaggttctg atataaaagt tgttaaagtt gtgagattcg aagttggtga
    32461 aggtatacaa aagagagaag aaaactttgc agaagaagtt gctaagcaac ttaagtaata
    32521 accaaagaga gaacacgcag gtgttctctt ttttaaaaaa tagaaaaaat tggatatgaa
    32581 ggattgaaat aggaggtttc tgatggataa accaatgtac aaaagggtat tgttaaaatt
    32641 aagtggagaa gcattagcag gagaaagagg ttttggtata aataatgatg tagttaatga
    32701 tattgctatt gctataaaaa aaatacaaga aataggtgtt gaagtagctg tagtagtagg
    32761 tggaggaaac ttctggagag gcagaaccag tgaaggaatg gacagaacta ctgctgacta
    32821 tataggaatg ttagctacag ttatgaatgc aatggcgcta caggatgcac tagaaaatat
    32881 agatgttgct acaagagttc aaactgctat agacatgaga caaattgcag aaccatatat
    32941 aagaagaaga gcagttagac acttagaaaa ggaaagagtt gttatatttg gtgcaggaac
    33001 aggaaaccct tactttacaa cagatacaac tgctgcttta cgtgctgctg agatggaagc
    33061 agaagttata ttgttagcta aaaatgttga tgcagtatat gacaaggatc caaaagtaca
    33121 tgcagatgct aagaaattta cagaattaag ttacatggaa gttatacaga aagaattaaa
    33181 agtaatggat tctacagcta catctttatg tatggataat aaaattccaa taaaggtatt
    33241 tgagcttaca acagagaata taataagagc agtaaaaggc gaaaatatag gaactactgt
    33301 aaaataatga agaatgaagg aggagtaaaa ttatgaaact ggaaatacat aagcaattag
    33361 aagagaaaat gaatgggaca attgatgctt taaagtttga atttggaact ataagagcag
    33421 gaagagctaa tgcacaaatg ctagacaaaa taagagttga ttattatgga actccaactc
    33481 caataaatca aataggtgct atatctgtac cagaaccaag aatattaatg atatctcctt
    33541 gggataaatc tgctatgcat gaaatagaaa aagcaatagc taactcagac ttaggattaa
    33601 atccatcaaa tgatggtgag gttataagac tttctgtacc tgctttaaca gaagaaagaa
    33661 gaaaagaatt agctaaaaaa gctagtaaag ctgcagaaga atttaaagtt agaataagaa
    33721 atgaaagaag agacgccaat gagaagatta aaaagatgga aaaaggcgga gaattaactg
    33781 aagatgaatt aaaaaaagca caagatgaag ttcaaaaaat gactgataaa tttataaaag
    33841 aaatagatac attattatct aaaaaagaaa aggatataat ggaggtataa tattgaaaaa
    33901 atattataac cttttaggtc ttcatgtaga tgaggtaaaa caatattttg atgagcaaaa
    33961 tataaattat accgtaaaat ctattgaagg taaaaaggat aaagaaaaac ttgtagttcc
    34021 tagagttata aaaatatctg agataggtga tagtgtagaa ctgataatca catacttttc
    34081 tgactcctta gtttaaggag tcagaacaat attggaggca aaatatgaat aacaacatta
    34141 tatatgatat agacttaaat aacataccta ctcatattgc tattataatg gatggaaatg
    34201 gaagatgggc aaaagctaga tttcttccaa gaactgcagg acataaggct ggagtagaaa
    34261 ctataagaga tatagtaaaa gaatgctcaa aattaggtgt taagcactta acattatatg
    34321 cattttcaac agaaaactgg aaaagaccga aacttgaagt tgacacactt atgaacttat
    34381 tatctacata cttaagaaat gaaatagctg aacttcacca aaacaatgta aaagtaactg
    34441 ctataggaga tataagtgct ttaccaaaaa cttgtataag agaattaaac tcagctaagg
    34501 aactgacaaa aaacaataca ggagttaact taaatttagc attaaactac ggaagtagag
    34561 cagatataaa aaatgctctt atagatatag taaaaaattg tgaaagtggt aaaatagata
    34621 taaacaatat tgatgaagat attataaaaa actatttaag cacaaagtct ataccagatc
    34681 cagacttggt aataagaaca agtggtgaac aaagattgag taacttttta ctttgggaag
    34741 ttgcttattc tgagttttac tttactgata tacattggcc tgattttaaa aaagaagaac
    34801 tacagaaggc tatatacgta tatcaaagaa gggatagaag atttggagga cttaagtaag
    34861 gagattattt tatgcttaca agaattattg cttcactagc tctggttcca ttatttctat
    34921 ttgttgtata tggaggtatt cctctatata tagctgagac agcaatagtt tatattgcat
    34981 tgcatgagtt ttataaggca tttaaaataa aagatgtaca tccaatattc attataggat
    35041 atttattttc tatttattta gctgtaaaaa atatttttaa tttaccatta gaatatactt
    35101 atgcagtaat ttttatttta tttttagcaa gtataattta tatgttgatg ggcaaaaaca
    35161 atgttataga tgtttcgatt acgtttttag gagtttttta tataggagta tttttagatt
    35221 ttataattat aactataaat ggatttgaaa aaggtagtat atatgtatgg cttatatttg
    35281 tgatatcttt tatgacagat atatttgcat attttagtgg ttatttactt ggaaaacata
    35341 agctaattcc taaagttagt cctaaaaaga ctatagaagg agctattgga ggaataatag
    35401 gaagtacact ttgttgtatt ttatttggat atttatttgg cattgatttg cttcaattag
    35461 cgataatagg aagtatagga agtgtaatag ctcaattagg agatttattt gcatcttcta
    35521 taaagagata tgtagggata aaggattatg gaaagataat acctggtcat gggggtatat
    35581 tagatagatt tgatagtgtt atattagttg caccttttgt atatagtgcg attaaatttt
    35641 ttattagata aagattggtt ggatttaaaa gagcaatcta taattgctag taatgaaaat
    35701 agctatgtaa cttttaaatg cagagatatt ctgcagttat tagttacatg gctattttca
    35761 ttgttcaaaa atttagaaga ccttggggaa ttgtataaga ataggtatta ctttataata
    35821 gtatactata tttgtagtga gtatattttt tatactgtaa ctatcattaa aaaatatttt
    35881 tttgttt
//
LOCUS       DAEMHZ010000027        35141 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000027 DAEMHZ010000000
VERSION     DAEMHZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.027"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(114..893)
                     /gene="hisJ"
                     /locus_tag="KPK72_001230"
     CDS             complement(114..893)
                     /gene="hisJ"
                     /locus_tag="KPK72_001230"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG2488464.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1439..1789)
                     /locus_tag="KPK72_001231"
     CDS             complement(1439..1789)
                     /locus_tag="KPK72_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG2488465.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1805..2221)
                     /locus_tag="KPK72_001232"
     CDS             complement(1805..2221)
                     /locus_tag="KPK72_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG2488466.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2240..2431)
                     /locus_tag="KPK72_001233"
     CDS             complement(2240..2431)
                     /locus_tag="KPK72_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG2488467.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2582..3397)
                     /locus_tag="KPK72_001234"
     CDS             complement(2582..3397)
                     /locus_tag="KPK72_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG2488468.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3444..4382)
                     /locus_tag="KPK72_001235"
     CDS             complement(3444..4382)
                     /locus_tag="KPK72_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2488469.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4379..5251)
                     /locus_tag="KPK72_001236"
     CDS             complement(4379..5251)
                     /locus_tag="KPK72_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2488470.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPYTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5276..7204)
                     /locus_tag="KPK72_001237"
     CDS             complement(5276..7204)
                     /locus_tag="KPK72_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG2488471.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWLSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7316..7693)
                     /gene="gcvH"
                     /locus_tag="KPK72_001238"
     CDS             complement(7316..7693)
                     /gene="gcvH"
                     /locus_tag="KPK72_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG2488472.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7780..9906)
                     /gene="cdhC"
                     /locus_tag="KPK72_001239"
     CDS             complement(7780..9906)
                     /gene="cdhC"
                     /locus_tag="KPK72_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG2488473.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(9963..10769)
                     /locus_tag="KPK72_001240"
     CDS             complement(9963..10769)
                     /locus_tag="KPK72_001240"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2488474.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10819..12186)
                     /locus_tag="KPK72_001241"
     CDS             complement(10819..12186)
                     /locus_tag="KPK72_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG2488475.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12217..13161)
                     /locus_tag="KPK72_001242"
     CDS             complement(12217..13161)
                     /locus_tag="KPK72_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG2488476.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13181..13951)
                     /locus_tag="KPK72_001243"
     CDS             complement(13181..13951)
                     /locus_tag="KPK72_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2488477.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14007..15392)
                     /gene="lpdA"
                     /locus_tag="KPK72_001244"
     CDS             complement(14007..15392)
                     /gene="lpdA"
                     /locus_tag="KPK72_001244"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2488478.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15436..16317)
                     /locus_tag="KPK72_001245"
     CDS             complement(15436..16317)
                     /locus_tag="KPK72_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG2488479.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16353..17027)
                     /locus_tag="KPK72_001246"
     CDS             complement(16353..17027)
                     /locus_tag="KPK72_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG2488480.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17064..17936)
                     /locus_tag="KPK72_001247"
     CDS             complement(17064..17936)
                     /locus_tag="KPK72_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG2488481.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18028..18660)
                     /locus_tag="KPK72_001248"
     CDS             complement(18028..18660)
                     /locus_tag="KPK72_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG2488482.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18765..20441)
                     /locus_tag="KPK72_001249"
     CDS             complement(18765..20441)
                     /locus_tag="KPK72_001249"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG2488483.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNVLRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKSDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20690..21469)
                     /locus_tag="KPK72_001250"
     CDS             complement(20690..21469)
                     /locus_tag="KPK72_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2488484.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21779..23698)
                     /gene="cooS"
                     /locus_tag="KPK72_001251"
     CDS             complement(21779..23698)
                     /gene="cooS"
                     /locus_tag="KPK72_001251"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2488485.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24305..25216)
                     /locus_tag="KPK72_001252"
     CDS             complement(24305..25216)
                     /locus_tag="KPK72_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2488486.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25332..25454)
                     /locus_tag="KPK72_001253"
                     /pseudo
     CDS             complement(25332..25454)
                     /locus_tag="KPK72_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(25537..26679)
                     /locus_tag="KPK72_001254"
     CDS             complement(25537..26679)
                     /locus_tag="KPK72_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2488487.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQATKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(26697..28451)
                     /locus_tag="KPK72_001255"
     CDS             complement(26697..28451)
                     /locus_tag="KPK72_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG2488488.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            28587..28793
                     /locus_tag="KPK72_001256"
     CDS             28587..28793
                     /locus_tag="KPK72_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488489.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(28798..29721)
                     /locus_tag="KPK72_001257"
     CDS             complement(28798..29721)
                     /locus_tag="KPK72_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488490.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(29975..31675)
                     /locus_tag="KPK72_001258"
     CDS             complement(29975..31675)
                     /locus_tag="KPK72_001258"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG2488491.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIKLLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(32136..32549)
                     /locus_tag="KPK72_001259"
     CDS             complement(32136..32549)
                     /locus_tag="KPK72_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2488492.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(32617..34995)
                     /locus_tag="KPK72_001260"
     CDS             complement(32617..34995)
                     /locus_tag="KPK72_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2488493.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
BASE COUNT        10380 a         7043 c         3874 g        13844 t
ORIGIN      
        1 ttattattat tttgtttaag ttctattact attttcaatc atataaattt acaaaagact
       61 gtacataaat ttattttata caaacttatc tacagtcttt tgtgtgaata tatctaaata
      121 atatcgtatt tatttatttc ctctgcacag tgagaatcag aacctaaaac taatggaata
      181 tcgtattttt ttaccaactc taataagtat ccatctatat aaggctcttt gcagtactct
      241 tttctatttc cagaaacatt ataatcaagt tcataacctt tttctttaat taactttatt
      301 aactcttcaa gaattgtatt tcctttataa tcgtatggaa atacctgatt aaactttcta
      361 actaaattta aatgacctat tctttttggt ttatgtatac ctaaatctga atttacagct
      421 tttatcaatg tttcataata cttattataa acagcttcaa tacttcctaa tttgtcgatt
      481 aaattttgaa actcctctac actgtaatct acacaataat aatcatcatc aatcttaatt
      541 atatgtactg ataaaagaga gtcttctaat tcttctccat atttatttaa attctccttt
      601 atcccttctt cgtatccttc tataaaatcc acttccgacc caacatttat cttgatttta
      661 ccctcatact tttctttcaa cttcttgaca tcattaaaat actttggtaa atcttcaata
      721 ctcatacaac tatcattttc aggagcaggg tctctaaatc catttgcata cggaaaatgt
      781 tctgtaaatg tcatttctgt tattccaaca tccattgctc tttgtacata tttttcaaaa
      841 tcatccttac ttccatgagg acaataaggt gaatgtatat gaccatcttt cataaataat
      901 tcactccttt tttaaagaaa aaatctaata taaatgcata aatcttttaa cttcaaaata
      961 atatttttaa tcatgctaat ttttattata tctaatatta gagtaatttt aaactgcctt
     1021 ttaagtatct tatcattagt actattttca acttaacatt aatattattt tttaacttaa
     1081 cattaatact atctttaaat agtaatactc aatatataat tacttaacat aaaataactc
     1141 aaattgcttg atgaagcttt tgcagtattt aaagaatata taattattta acattaaatt
     1201 atccaatctt gtaataaaat ttactcatta aagtatatta cagaaaatac ctaaaaataa
     1261 actttttagt tcatgagata ctctttttta catgaaatta gatatatttt tatcatttca
     1321 acaatgaaag ggaggcttat atccatcttt aaacaacccc ttagataaaa tttttaataa
     1381 ttaaacatca caaaatataa aaatcgctta tatcaaacgt ataagcgatt aattatattt
     1441 acatatgttt tttagcctct aacaacattt tattagcttc ttccatatac tctatagcct
     1501 tttcaatact ctttgctgtt tcttcttttc caatagctct agccttttca acccactctc
     1561 taaatccttc ttcatgagtt tcattgtgat ttatccaatg tactaaaagt atttttaaag
     1621 ttttttcatc cttagactct gatgattcct catgactatg ttcgtggtcg tggtcgtgag
     1681 catggtcatg tggatgttca tgacatatat ctccatgagt atgttcgtgg cagtggtcat
     1741 ggtcatgatg tccatgatga tatcttacaa tctctttatc aaaaatcatt agttattccc
     1801 cctgttattt tatattaaac tcttttttta taaggtttac agaatctttt acaagtagtg
     1861 ctaatggttt accttctaat ccaactaact ccacattttc tggcataata ggtaaaagta
     1921 tcttaagtgc ctcactgtct gcaatagctt cacttatatt acaagtaact tctcccatca
     1981 tagaattagg tattattaca gaaataggtg caactattat atttgctttt ttagctgata
     2041 ctactatagc attttcccct gtagctcctt tattagcatg agctttcatc atagaactag
     2101 ttgctataga attagtccct agtgcataaa tttctacata tgtaggtaac tcttctctca
     2161 aagaagaaac tatctgggct ccaatacctc cacccattcc atctatcact gctattatca
     2221 tatgaaattc ccctattaat taattttctt gtattattat cttatggtcc atcaatctta
     2281 tttctttaat tatcccatct attatctttt gctctcctaa taaatccgtt aaataaatct
     2341 ttccatcaca agggtctata ttaaccacat tttccataac cttttctaac tcattattac
     2401 ttttacaaat gaaagctgat gattcacaca tataaatctc ctcctaatat gatttttatt
     2461 aaaaatcaaa aaaagattcc cttaggtatt tcaatacacc aaagaaacct tcatgatttc
     2521 agtttttgta tattattata ccttaagtat atcactttaa tttatgtaaa gtcaattaaa
     2581 gttaacgctt tttattatac tcttcattct tgtatttttc atatagttct ttaactaatt
     2641 gcttctcata atcagtcaaa tcgccctcca caaactcaac attaaattgt tcagctaatc
     2701 cctttactat gtttttctgt agaatatcta tgtctatttt tctgccaatt tcattttcta
     2761 ttccacttgc ttttttagct gtaaatttca tagctctttc tttcaattct ggtgtcttag
     2821 tttttattaa tttaaacaat ttttctacat caaaatccag tattattgaa ccatgttgta
     2881 ataaaacacc atcttttctg ctttgagcac ttccaattac cttcttatta ttaatagtaa
     2941 cttcatatga tgcatgtgca ttaaaacatg cagctgataa attttcccta cttattctct
     3001 cgcctctatt taaattatct gtttctatac cacttagatt caatccttta actagacctt
     3061 cacttatata tctataagat aggttgatgc tcttatcgat taaaggatta tcttctccta
     3121 taattatact ataggttagc tcattatcat gtaatacagc tcttccacct gtaattcttc
     3181 tagtatagtc tatattcata catctacact tatctaaatc tatctcatct tccaatttct
     3241 gaaaatatcc tatactcaaa caagctggtt cccatgtata aaatctaaga gttggcttat
     3301 tatgtccttt cttatatgca gtaaatatag cttcgtcaat tgccatattc attgctcctt
     3361 catatgattt attatgtata actctccact gattcatgat tattacgccc ccatttttag
     3421 tataaatata tcctagaaat cctctataga tttatttctc ttattgctaa atctatctca
     3481 ctttgattta aaggtcttga atagttgtat atagtacttc ctggtctttc attatagtat
     3541 ggtctattac aatccttaca acctcttatt tcaaaaggat agcctttgtt tatgtctttt
     3601 aaaatatcat tgtcaagctt tatactattt aagtatccat ttttaaactt aaaatactct
     3661 tttggataac ctttatttat catataagac attaactgta cccttctata actttctatg
     3721 cttggttgac ttatcttttc cattttagtt cctctaactg gagtaaatgc aaataaacta
     3781 atcgttacat cattttcctt taaataagta tataaattgt atatatcttc atgactttca
     3841 cccataccaa ctattatatg tgtacttatt ttattgggat atgattttga catttcagtt
     3901 ataaatttta acttttcatc atagttattt ccttttattt tttcatatag ttctttattt
     3961 gctgcatcta ttgctattcc tattttatca actcctactg aaaaaaactt ctttatctgt
     4021 tcatcatttt ctaattttgc agatagtgaa attggcatgt ctatcttacc acttatatag
     4081 tttataaagt ctaaaacaga gttatgtgct tcttcacttg ccattgattg aatacatatt
     4141 ctctttatat tttttccttt ataagctttt aaagcatcta atatttcttc ttttgaaaac
     4201 tctggccaaa ttactcttga taatttatct tttctagtac tactttctat tgattgagaa
     4261 caaaaactgc atttatttat gcatttttct ccaatcatta tatatgctgt ggtaggaggt
     4321 atatcgcttt ttttgtttaa tatcccaagt tctattgctg tcccaactga tagtctaatc
     4381 ataaaacgca acactcctta cactcattta ttgtcatatt catttctata gccttattct
     4441 ttgcagacct tgatggtaat actattctat tgactccaca cattatagat aattggtcta
     4501 gctcttttct atacactcct cttggtctca tacaacctag attaatttca gtatatggta
     4561 aattaattct agcttcacat aaaatatctg ctaccccttc taactcagga ggttctacat
     4621 tttcatactc agtccctttt gttggaataa ggactataaa tgtaagctta ttaggaggat
     4681 ttttttgtag gtattctata atttcatatt caccctttat ttgtcctcct tttaaaccaa
     4741 tacatatatg tggtgctact tctgtatgtt cttgtattgt attatagact tcaatataat
     4801 cttctttact ctttttcatc ttataaactt ctcttattgt ctcatcatca aacactaaat
     4861 caaaagaaac tatatctaaa tacttacaca actctacaat gctatcttta tccataagac
     4921 ctaaatgagc attcaatcta tatccttgct cttttaagtt tttaatggta ttaatatgag
     4981 tatttatagg gacatctccc tcataactac aaccgccact caatagaaag ctagttatat
     5041 tccttgattg tactttttcc tcatattctt ctattggcac catattgttt aagtagtgac
     5101 cgttgcaatg ggcacatttc aaactacatt gattgcttgt agtactaaga gctagtgtct
     5161 ggttaggata agcaaactct attttatttc caaaattctt taatttaata tcaaatgcta
     5221 ttttcatttt ttcagataga ttcttattca tagtttattc cttcttaagt acattttact
     5281 cttctagtaa tttctttgct gttgggaact gctctatatt cgtatgtggt aagaaacatg
     5341 cagaaataaa ttcatccata taacttggat atacactaag ttcaacataa gttatttgat
     5401 tacctatttc ttcaagttta ttttttgcat ctttacttat aagagctaag taagaaccaa
     5461 caagtgaact attccctata taagtaaatt tttctctatc gatatcagga agtaacccta
     5521 taattattga gttttctatg tctaggttgt ttcctatacc acctgctata taaaccctat
     5581 ctaatatact aaaatccata cctaaactat ctacaagagt gtatgcgcct gaatatatag
     5641 ctcctttagc tcttatgaaa ttatctatat caacttcatt gactactata tcattttcta
     5701 aatcaaattc ttctttaaat gctaatacat actctcctat ctcatgctca ttaaatctaa
     5761 ctctcttatt atctaaatcc ctgtatattt tacctcttct atctattaca cctttggtaa
     5821 tcatttgaca gattaaatca attataccag aaccacatat accaactggt gcacattcat
     5881 caataatctt taatgttggc tctaaagtat ctttatcaat aattactttc tctattgctc
     5941 ctgctgatgc tctcattcca cagcttatcc ctccaccttc aaaagcaggt cctgctgaac
     6001 atgcacagct catcatataa tctttatttc caaatacaat ctctccatta gttcctaaat
     6061 ctataaataa aacattttct tcacttaacc atatccctgc tgataataca cctgctgtta
     6121 tatctcctcc tacataactt gcaacagatg gagctaagta cacatatgca ctgtcattta
     6181 caaaaagacc aacattttca cccataagtt ttggagattt taaaaatggt ggtatgtaag
     6241 gttcttgtct taagaagtct gtatacacac ccaagaataa acttgtcata gttgtattcc
     6301 cagcagccac taatgtaact acatcttcct tatctattcc atttctttca tatattgttt
     6361 ttaatagtgg atttatagtt tcctcaacta tagctttatg aagtgtctct aatccatttt
     6421 tcttagttga atatataatt ctatttataa catctgctcc atattttatc tgtgcatttc
     6481 cagaagatgc tttatctact acctcttttg agtataggtc tactaaacaa acaactactg
     6541 aagttgtacc aatatctatt gcaactccgt ataaggaatt ttctttattt ccttgttcta
     6601 tatttatgat tgtaagtttc ttttgttttt gaacatatgt tattgtaacc ttaaaatcac
     6661 tctttctaaa tacagttggc atttttctaa gtatatccaa tctaaaatct atttcattat
     6721 atcctaggtt atttctaaca tatctttcca atctatctac atcacttata ttatcatcta
     6781 aatttggttc ttccatttct atgtatttct tttttatatt tgttttaaat tgaaggttat
     6841 gttcttcaat tattttttta gctctttcaa aaatttccct atcctctttt ttattactac
     6901 cttctatctt cataccatgc atagaagatg atactttaga aggaacttca atttctatat
     6961 ctgatattac ctttgtacaa caagctagta tataaccttg ctcccactca tcatctgtta
     7021 tatgtcttgt tttttctgta tcaacttttc cattcaataa ttttacttta cacttcccac
     7081 aagacacgtt tccattacaa ggagcatcta tgaatatatc tgcatttctt gcaacctcta
     7141 gtaatatatc cccctcatta caataaacct ctttattgtt tggtgtaaaa cttactttta
     7201 tcatgttatc cctcccaatt tgttctatat atgataaaag gaaagtatag ctaaatttgc
     7261 gtgcactcaa attttactat actttcccca attataataa tatcttaatc tttaattatt
     7321 ctaatccagc ctcgtattct ttatcactta ataacgtatc aagttcagcc acatcagcta
     7381 gttttacttt taatatccag ttttcatatg ggtcttcatt tatacattct ggctcatctt
     7441 ctaatttttc attaacttcc aatacctcac cattaacagg agatattaaa tcagaagcta
     7501 ctttagaaga ctcaactact ccaaaatcta ctcccttagt tacagtatct tctacttctg
     7561 gtgtttcaac aaacaatatt tctcctaatt gatcttgagc atagtctgtt atacctatat
     7621 atacaacatc tccatcaatt acttttaccc actcatgatc tttagagtat tttaattctg
     7681 gtaataattt catttcgata gacccccaat attatattat tgattaaatt acttaaatca
     7741 tattaatatt atatcatatt attttaaatt ttccatcaat tacattacac tttccatagc
     7801 taatgctgga tgaccttttt cttctaagaa agctaatact ccttcagaat cgtcagctat
     7861 agtttcatca caaaccatat cacagaagtt ttctattcca gtcatttctt taactgtagc
     7921 atttaattta tcagctacat aatcttttaa ttcttttggc atccaaacta ttctttctgg
     7981 tccaccttct gcataagcga attttttaga agatatgaaa tgtcttccgt gtcccataaa
     8041 tcctggagtt tgaactcctc cacctgtcat agaagcaagt tctccgaaag tcataccaac
     8101 tggagtaaca cttgcaaatt ctctgttaac tacaacaaat ccatttgctt ctggcattat
     8161 accacagata cattcgaagc atccacaaga agtcattggg tcttctagta tagagtataa
     8221 tgtaacagat tcaactgcac cttgagatat ttggtttaca gtttcattta ctgaattcca
     8281 tacacctgtt ctatcatcta agcactcgcc tttttctatt ggttgacaag gacctgttgg
     8341 gtctaattct ttagtagcct tagcatctaa ccagctaact gctccacaaa gaccaagtct
     8401 ttcaggagta acaacacaaa cgtgagctgg tgcaaatgat tgacataagt tacaagaata
     8461 gaaagtgtca acactttcat caactaatga agctaatctt tcatctctag cattgtactt
     8521 agctattgct tcacctttta attcagaaac tttttcagca tctgtaatta tagttatttc
     8581 acacttatca actactgatt caaattcatc taacatctta gcatataaaa cttctcctat
     8641 atgctcaagt ctaaatcctt tatcaaatgc atctttagag attcttaccc aagccatatc
     8701 tctttgtcca acgtgcatta caccttctat atagtttaag aagtagtgga aacgtctttc
     8761 aagaactggc tcgaaatctt cttgcatatt tttaccagct attttaacta ttacagcaag
     8821 tggtaatctt aatacaccat ctgcatcaac ttcatctata tttggtccta ttatttctat
     8881 cttatggtct tcaacttctg ttgcttcttt cttaacaaca agctcccaag cttcagttct
     8941 gtttccacca aactcagcaa acatatcgct ctttctaatt ctctcacctt caaatgctgc
     9001 tgcaaatgaa actgggatag gtatttcagt aacttttatt tttattcctc tagcttctaa
     9061 tgaagtagct actatcttat cgtaatcttt ttgagttaat aggttcattg gaacttcaag
     9121 aactgtttgg tcagttataa ctgggaatcc taaagctatt gctccagcac ctgcagatac
     9181 aactaattca cttaatggtc caaatgcatt aacaaatgca ggaactctat tagctgtgta
     9241 ctctaatagt ccgtttaaat caccaggagt taatccaccg aatattaatg ctgctcttac
     9301 tgcaacagat acaacgtgta taacagaagt tacatcatat cctagaggta taactcttag
     9361 ctcaagaccc atttttactc cagcttctat agcttggtct ataactttac caactaagaa
     9421 agttagtaaa cctttagatt ggtaatcttt tataactttt gctgctgatt ctgcatctgg
     9481 acactcacca agaacaactg caacaccagg tatatctcct gtaactaatg gtacaccaag
     9541 tgatctgatt atagggtcag ttatatgacc tgcacatggc tcactatatg gagcatccat
     9601 agttgaatat tttaatgctt caattacttc tgctgctaaa gctgtagcta aaccagcatt
     9661 aaaagcatgt tctagtaaat gagttctatt tattaaagat tttactactt ctaaagcacc
     9721 ttctaagtcc cctaaagtgt tcatcttttg tcctgttgca gcatatatac aaggtaatga
     9781 atatgctgta tcagggaaag caactttatg ctcttttcca ttcttttcta tagcttcagc
     9841 taacatagct tgagccgcac ctaaagcttg ttctgaccct gtaaagatta tattatatag
     9901 attcattcta tttccccctt taatatttac acctattttt tattaactat accctaaaaa
     9961 ccctagaaag caaatccacc ttcatttaac tctaagaaag agtctgcata taaagatgcc
    10021 ccgttgatta tgtcacaaga ttttacagtt ttcattaatt ctgggtcaca tggattcatt
    10081 atagctgaac taaatccatt agccattgcc attgctaaga atgcactatc taaaacaggt
    10141 ctaacatgct taggacatcc attagatacg ttagataatc ctcctgtagt taaaagtccc
    10201 atctcagtca tcatttttat agcttctaaa acttctactt gtttttcttg catacctttt
    10261 ataactaagc ataatgggtc aaatagtata tcagttggct ccattcctaa cattaagcct
    10321 ttttctaata tttcttggca gtaagccata cgctcatcat tatctcttgg gataccttct
    10381 tttgcacata aacctataac cattgcttcg tattcagctg ctatatctat taaatcaaat
    10441 cttgaaccag catcagcaga gtttattatt ggttttgcat tagttctatc ataaactttt
    10501 aatccagctt cgatagcttt tttattagct gtatctaatg ctataggaac attgttgaac
    10561 tcagattgaa gtaatttgat tccccatgtc attatttctt cgccatctct ttcggcaggt
    10621 cctatattaa aatctatata gtgtgctcct gcttctaatt gctcttttgc tctttttaag
    10681 attggagctg ggtctctttc tgctaatgct tttcttattg aaggtgatat acaatgtatt
    10741 ctctcaccta taatcataaa tttttccatt tataatgtcc ccctttaaat ttaatattaa
    10801 tagttagtta atattttatt agtttgtttg gcatgctttt tcttggtatc cttttaagaa
    10861 cttaggtaat tccatagatt cctctggtcc tataactaca ttccatcctg gtaagttatc
    10921 ttctatatca cctttaagta ctgcaacttt tcctggaatg ataaggtctt tacaattagt
    10981 tacttcttct accttacttt ctttaatgaa agcagagatt gagtttccac caaatttacc
    11041 agcagcccaa gatgtaagaa ctgaatatcc acctgcatct ggtattacta accatactgg
    11101 aacttttgat ctttctatat caccagcaac tatgaagtaa gttaatgcaa agtcaacagt
    11161 aactaatact ggagaatttt catctgggtt gttgattgga taaatctttg gttcaactct
    11221 cattggtctt tgtgggtcag tgtatatgtt ttgtcttagt gcaaataatg gtaatgcctt
    11281 agcataacta atatcatcta ttactattat agaaccgtat tttatagtga atattgatga
    11341 taaagcaact tccatcatag ggtttgaatt agataatcta tttgcaaata ctattgaagg
    11401 atatccaaat gttctatctt gctcttttaa agctgttctt ctaacttgta tagcattagt
    11461 gtaagtatct tttatgtttt ctcctgttac atctaatact aattctttat atccagcagc
    11521 ttgaattaat tcaactgttt catataattc ttctaagcta ccagctttta cacctaatgg
    11581 taaacttgct cctttaacta cttctatcat atctttgtag ttctctttag ttgctccata
    11641 taccattggt ctagcatctt ttaatacttc aacagcttct ttaactactt gtgcatcatc
    11701 acaagctaaa atataagcta cttctaatcc actgcctttt attttatcta ttaattttaa
    11761 atacgcatct ttatctccac aatactctaa aacagccatt tcaactttca tttgttcacc
    11821 aattcttaca tagtcaactt ttttcatgtt agctatttta gcatctactg cttcatcact
    11881 catacaagta cagaatgaaa ctgcatatct atttctactt actaatgttt tttcatgtct
    11941 aaataatact gtttctccac ctagttcata ctcagatgca ccagcaccaa cttttaaagc
    12001 tttcattaaa ggtgcagtag cctctgataa ttttgctata gcgtcatcag acatatgtgg
    12061 gcatttacct acttctacag ctcctgaagc aaccttcata gagaaagcca tacaagttgg
    12121 gaatccacaa tccttacagt ttttctttgg tgttaattta aatatatcta aagcttttaa
    12181 tgccattatt ttttccccct aaccttaaca atttaattaa gctaatgcgt taaccaattc
    12241 ttttatagtt tctattgatt ttggatgacg aagtataact gcgtttgaac cacctactaa
    12301 aacacttgct gcagttgaaa cttccattgc tatagttctt tcttctggac aaccccaatc
    12361 tggctcatct tctattggag ctatagcttc tttaacatga cctacttcaa aagctactgg
    12421 tgttatgata ggcatttgta atgtcttatc attttgacca aatgctgcaa gtctaattct
    12481 atccatagta gatgcaacgt actcatatcc ataaccaact gctgaacatc caacattcat
    12541 aactatattt tcacctttaa cgcctagttg agttaacaat acgtttaatt gtttagctag
    12601 gtttatatca acagaagatt cagctcctac tttatgtgca taagccatac cagctgatgc
    12661 tcctactcct ttgtagttat cttctactgc tgacataaat aagcagttat gtccatccaa
    12721 tgtttgagct aatttttcaa ataatttacc atctttttca tgatttcctg aaccagctac
    12781 aactaaaggt aattttattg cttcaattac tgccttagct acatcagcac attcatcaac
    12841 tgacttatct aatccatttg ggtcagaacc atcaaatttt aaacaaataa aatctgcttg
    12901 tgtattagct tcaacatatt tagcccactc tactggacaa ttagctacat ctttgtataa
    12961 ttccttatat gaatcagtcc agctttcagg ataaacgtct gatatttgta tacctatttt
    13021 tggagaatta cctacttctc catcaaaact ataaaaaggt aatacatttt ccccacctaa
    13081 tttaattgct ttttccccta ttcctacttc aacttctgat atttttccag aatatttttg
    13141 agtagacatt ttaaatgcca tatttttttc ccccttaagc ctattttaat tctaatttag
    13201 caaaaatttc tttcatagcc actttagatt ttgaatcttc aggtaaatta actaaaggaa
    13261 tacctgtaga atcatattca tatatcaatt catccatagg tacaacaccg attagctcta
    13321 ggttatgctt ttcaatttct tctttaacac catcattcat aaccccattt ggaactttat
    13381 ttacaatcaa aagtattctt ccaacactta actttagttc ctcagctaaa tctcttattc
    13441 tagcaacagc ttgtatgcta cgtcttgagc aatcactaac caataaaagt gtatcaacat
    13501 gtctagtgac ttttctactt agatgttcca taccagcttc gttgtccatg actaagtatt
    13561 tgtaatgacc agatattttg tttacttgtt ctctaagtat cccatttaca aaacagtaac
    13621 atccttcacc ttctgaccta cccattacta ataagtcata tccttcgcct tcttcgatta
    13681 tagaatttaa tctaaattga agatattgtg cctttgtcat accaccaggg aatgcattgc
    13741 ctaacttttc tctttgattt acttcttctc ttatttctcc tattgttgct tcaacttcga
    13801 tacctaatac ttcatttatg ttagcattgg catcagcatc tacaactaat actggtccct
    13861 ttttgtcctt aaccaaataa tcaatcaaaa gccctgtgag acttgtttta ccagttccac
    13921 cttttcctgc aacagctata ttgtatccca ttttaagact tcccccttaa atctagccac
    13981 agagccttat gtactctgtc tgtctattat aatttaggta cagaatgaac acattctcca
    14041 tgtacatcat gaagtgcttc cattagccct tcagataaac ttggatgtgg atgtattgca
    14101 tcccctactt gttctactgt taatcctaga tgaacagcaa gtgacaactc tgtcaataaa
    14161 tctgtagcat gaggtcctac tactgctgca cctattatct tatcagtttc tttgtcagct
    14221 ataactttta caaatccttg gaaatgtcct atagcttgtg cttttccaag acctctaaaa
    14281 tcaaattgac ctacattgta ttctacacct tcagcttcaa gttgcttttc agtcttacca
    14341 acaccagcaa cttcaggttc agtataaaca catcttggaa ttgctctata atctactacc
    14401 ttagtcttac ctaaagcatt ttcaactgct acgatacctt ctttagaagc aacatgtgct
    14461 aggaaaggag tatctattat atcgcctatt gcgtatatac cttctacatt agtttcaaga
    14521 tgttcattaa ctactacttt tcctctttcc atttcaattc ctatatcttc tactccagaa
    14581 ttgtcaaggt taggtcttct accaacacat actaatgcat attgtgcttc tataaccttt
    14641 ccattagaaa gagttgcaac agccttacca tcaactactt cacaagtttg tactccaata
    14701 cctgtaataa ccttaatttt atctttctta aattgtctaa gtagttgttt agctacatct
    14761 ttgtcttcat ttaatagtat ttggtcaacc atctcaacta tagttacttc tgttcctaag
    14821 gctctaaaga actgccctat ctcacatcct ataactccac cacctactat taacatagac
    14881 tcaggtatct cttcaagtcc aagaacttca tcactagtta ttacaacttt tccatcatat
    14941 gggaacattc ttggtacaac tggaacagaa ccatttgcta gtattatctt atctgctttt
    15001 atagtttcta ctgttccatc atctttagtt acttctatag tgtttttatc aattaatttt
    15061 ccaaaaccat taactaagtt tactcctctt ttttcaaata agaactctat accacttatt
    15121 aattggttta caactttatt ttttctttcc attatggcag tgaagtttgg cttaattgta
    15181 ccatctattt ctataccaaa atcctttgct tcttttacag tattcaatac tcctgaagaa
    15241 gcaagaagtg ctttagttgg tatacatcct gcatttaagc aagttcctcc aactcttctt
    15301 ttttcaacaa ctgttacatc tgcaccaagc atagaagctt ttatagctgc tacatatcct
    15361 cctggtccac cacctactac tactatcttc atctttaatt cccccaaacc taatataatc
    15421 tacaatcttg caatactata aacctgcggc atccagtata gcaggtatat tctcttctgc
    15481 cccaattgcc attatatgaa caccatcaca taaatcttct tcttttaatt gtttgataag
    15541 ttcccctgcc atttttattc cttcacttac ccagttttct tttccagctg ctcttaatct
    15601 ttcgatttgt tcatctggaa caaatattcc tggtacattt gcagtcataa atttagccat
    15661 tcctggtgat tttaaaggta ctattcctgc taataccttg caatccatat cccttgttaa
    15721 ttctctaaac tttctcattg tatttatgtc ataaagtgct tgtgtttgga aaaatttagc
    15781 ccctgctttt attttctttt tcatttttaa taactgaact tctattggag aatattctgg
    15841 agttactgat gctcccagat aaaaatctgg agaacctttt aacttattac cagccatgtc
    15901 cgtacctgcc attaaagtct cagcagtttg aagtatcccc acagaatcta aatcaaacac
    15961 tcccttagct tgtggatggt ctcctacgct agtatggtct cctgttagtg ccaacagatt
    16021 gtttatccca aatacacctg ctgacaacat ttctccttgt atagctattc tatttctatc
    16081 tctccctgtt atttgaataa ctggctctaa tccagcatct tttaataatt tacatgtagc
    16141 aagagaagtt gctctcataa ctgccgattg aaagtctgtt acatttgctg catgaactct
    16201 tccaactaac ggctttgcac attctattaa atgtgaaagg tcagtccctt ttggaggagc
    16261 catttcagta gtaactgcaa attttccact ttctaatgtt tctcttaata agctcatctc
    16321 taatccccct tttaaccttc taaaaaaaac ttttatgaat ttgctgctgc ttctttcttt
    16381 ttctttgcac ttaaacttct tggattattt tgcttagaat agtcttttgg tggtcttatt
    16441 tctgctaagt tatctaattg tcctatagct tctagtcttt catatatctt tatccaagca
    16501 caatcattct ctgagtttac ttcacactta ccatttttag ctccaccaca agccccattc
    16561 ataagacctt tagcacacat agttactgga catatcccac ctgtccaacc aagttcacag
    16621 tctccacaag ccttacaggc ttcttcatat tctccaactc tttgtacttc ccctataaac
    16681 aaagtattgt tggctggata aactggctta tcttttaaat tcttaactat tgtctgtgta
    16741 ccatctccac atgctaaaga taaaactgca tcagcttcct tagtctcttc ttttagagcc
    16801 tttaaatcct ttttagactt taaaaggtta caagcagggt caagcattac atatcctaag
    16861 atttttttgc cttcaccttc aagagtttcc ttcattttta aaacttcttc ttccccacca
    16921 cttttacaag ttgctgcaca ttgattacaa ccaactaaaa ccagcttgtc aaaatctttt
    16981 aagtacccta gcacttcttc taatggttta ttttcagaaa ttatcatatc tacttccccc
    17041 ctaaaccttc catatatata tatctattta ttgttttgta atttacaagc cttaactaca
    17101 tgttttgcaa gtatagatgt tgtaactgaa ccaacccccg ctggaactgg agttatcatt
    17161 gatactttgt ctaaaactgc atttgtatca acatctccac ataaatttcc ttcttcatca
    17221 acatttatac caacgtctat aactaccgct ccatctttaa caaaactttc atcaaccatc
    17281 ttagctctac caatagctgc tattagcacg tctgcctctg ctgctacacc tgatagattt
    17341 ttagtttttg aatgacatat tgtaacagtt gcgtgttcac ttaataaaag cattgatact
    17401 ggctttccaa ctaccattga tcttccgaga actgttacct tacttccttt taaatcaaca
    17461 ttatagtgtt ttaatatctc aactactgct gtaggtgtac atggtggaaa tccactttta
    17521 tccccttcca ttaccttagc agagtttatt ggactaaaac aatctacgtc tttttccggt
    17581 gcaataacat attttataac ttcttcattt aagtgttttg gaagaggtct aaaacataat
    17641 ataccattaa catttttgtc atcattaact ctctttaata catcaatata ttcctcttgt
    17701 gttatatcct cagctaactc taacacttct gttgttatac ctatgttttg acatctcttt
    17761 agtgctcctc tttcataaga taaatcatca cttcttgccc caactctaac tattgttagt
    17821 ttaggattta taccttcttt aattagtaaa tcgacttctt ttattaattc ttcactaatc
    17881 ttatcagcta caggttttcc ttttataatt tgtcctttag ttgacattcc ttccatacat
    17941 tcacaactcc cctacaagca aacttaaatt aatttattct atataccaca tattaataac
    18001 atagtatcta ctttcgtcat ttaaaaatta ttttccaaga gctttttcaa ctttggcata
    18061 aacttcatca caaattttta caccatcttc aactaactta tctacttctg tttttacctt
    18121 attaacatat tctttatctt gtatagaatt tatatttata actacattta attgtccacc
    18181 aagtatagca gctcttaaac actgaactcc tacaccaaca tcacttatag caagtcttga
    18241 acctttatca actaaatctt catgtaactt aatagcttca tagcaaactc ttactatgtt
    18301 taatggtact tcacaagcaa cttttaatgc cttttccatt gtttctgctt ttattctttt
    18361 ttcttcttct gtttctttag gaagcccgta agcttttgat aatggtaaga aacattctgc
    18421 atcatcatct atcattttta ataaatcttt ttctaacttt cctgccttac ttaatatctc
    18481 ttttacactt tcttcatact cagcatactt cttcttccct attgtaaggt tacataccat
    18541 actacctaaa gccataccta ttgcacctac tagtgcagca gctcccccac cacctggtac
    18601 tgcttctttg gaagatagta cttctacaaa atcaacacaa gttttatctg ctattttcat
    18661 gataatcctc cttgaaagga tgtaggaaaa tatctcaaat tgttcgagat attttacgaa
    18721 ctattttaag atatctccta ctctttattt ataaattaat ttccttagaa taatccagct
    18781 attactccat tttcatcaac atctattttt tctgctgctg gaacttttgg taatcctggc
    18841 atcttcatta tttctccagt aagtgcaact ataaatccag ctcctgctga aattgttgct
    18901 tgtcttacag ttattctaaa tcctgttggt ctacctaatt tagtttggtc atcagttaaa
    18961 gaatattgag tcttagccat acatactgga actttagtaa atccaagttt ttctaagtta
    19021 gctatttcct tatcagcttc aggagtataa tctacaccat ctgctccata aatcttagta
    19081 gctatagcat ttatcttgtc ttttatagat aaatcatctt catagcagaa tctaaagtta
    19141 ttttcttctt catccaataa tcttaaaact tctttagcta ctgcaattcc accttctcca
    19201 ccttttgccc atacttcaga aagagcaaca ttaacaccta attccttaca cttgtcttca
    19261 acaagtttaa gttcagcttc agtatcaagt gggaatctat taattgcaac aactgctggt
    19321 aatccatata cttgagttat gttttcaaca tgttttaata agtttggaag tcctttttca
    19381 agagcttcta ggttttcgtt atttaagtct gctttagcaa ctccaccatt gtattttaat
    19441 gctcttacag tagcaactat tataacagca tctgatttta agtttgccat tctacatttt
    19501 atatctaaga atttttctgc tccaaggtca gcaccgaaac cagcctcagt tattacatag
    19561 tctgcaaagt gcatagccat tcttgtagct ataacagagt tacatccatg agctatattt
    19621 gcgaatggtc caccgtgtac aaatgatggt gttccttcaa gagtttgaac taggtttggt
    19681 tttaaagcat cttttaataa tgcagccata gcaccattag cttttaattg tctagctgtt
    19741 acaggttcac cttcgtaact gtatccaact attatatttc ctaatctttc ttttaaatca
    19801 gatatatcac ttgccaaaca gaacgctgcc attatttcag aagcaacagt tatatcaaat
    19861 ccatcttgtc taactgctcc atcacctttt ttacccatac catcaacgat atttctaagt
    19921 tgtctatcgt tcatgtctac acatcttctc caagttattt tcttagggtc tattctaagt
    19981 acatttcctt ggtgaatatg gttatcaagc atagcagcta aaagattgtt agcagctcct
    20041 atagcgtgaa aatctcctgt gaagtgtaag tttatatctt ccataggaac aacttgtgca
    20101 tatcctccac cagctgctcc acctttaaca ccaaatactg gtcctagaga tggttctctt
    20161 aaagcaacta atacattttt gtctagttta gcaaatgcat cagcaacacc tatagtagta
    20221 gtagtttttc cttcacctgc tggagttggg tttatagctg tagttagtat taacttagct
    20281 tttttaccag tctctctttt tagtagattg tagtcaactt ttgctttgta tttaccataa
    20341 agttctacat catcttcacc tagtcctaat ttttttgcaa cctctctaat atcttgaggc
    20401 ttagcttctt gagctatttc aatgtcagat ttaaatccca taactgaatc tcctcccaca
    20461 acctaaatag atttaaaata taatttttgt aaaaattgta ttaatatgta aaaattatat
    20521 tttataagat aattatacat gtaaaattac ttaaaaacta gctactaagc tattaaaata
    20581 ttcaaactta atagctagtt atttatctaa ttacttactt attacctatc tattgcctat
    20641 gtctaacctt taaccaactt aaaatcccaa atcaaaattt ctatctattt tatttatcct
    20701 taagtgacat taatttatct tttattgctt taatctgttc tttcgttgtt tcactagaat
    20761 catatggaga ttgattagct ctatcagaat caataacatc ttggttatag taaataaatc
    20821 ctaaagattc tccatcttct aagttttgga ttataaattc ttcatcttcc ttatttctaa
    20881 ttttatttcc tactacaaat actttattta cacctatatc atgtccaagt ttcttaactt
    20941 ttctataagt ctgtagactt ctctctcctg gttctacaac tactataaaa gcatctacac
    21001 cttgggctgt tcctcttcct aagtgttcaa ttcctgcttc catatccata accactacat
    21061 ctttattttg tagtattaaa tgtgagcaga gtctcttcaa aagaacatgc tctggacata
    21121 cacatccagt accaccagaa tctacggttc ccaatgtaag taatcttact ccattgtatt
    21181 ctttacagaa attttcaggg atatcgtcta ctttaggatt catcttaaac atttttccaa
    21241 atgaacctac agaagcagct gttctatctg aaactaattt tttcatttct gatataggta
    21301 ctattgattc atatacttct tttggaaaac ctagtgctaa agctaaatta gcatcagggt
    21361 cggcatcaac agcaacaact ctgtatccgt cttcagcaaa catcctagag agcattgaag
    21421 aaaaagtcgt tttacctact ccacctttac ctgttattgc tatcttcata atccctacca
    21481 ccttttatta ctatttgctt tttagtagtt aattttacaa ttaaatattt tttatactct
    21541 atatttttat acactaaaaa taaactacag ctagtcctta caggaaccaa ttaaatttac
    21601 cctactagct tttcaaagca gcatgtatag ttttattatt ctttaatatt gtttatattt
    21661 tatttattca gtatattcat tatatttcga tgttctttac tttaatattt gactttattc
    21721 aactttactc aaccagaaac ttcttataaa aacgtttatt ctataacttc actatttttt
    21781 aagcttcagc cttttctgcc attttttctt caactaatgc ttcaaagtga acacgttttt
    21841 tctctatatc ttctattatt gtagctgcta actcttttgg atttgtattt acagtaaatg
    21901 tagctccaac tttatctttt aactcactag ttaatatatc cactgctctt gaagaaccag
    21961 ctattggagg cattattcct aaataagtgt ctataccaga agctacaaca tagcatccta
    22021 tagcaactgc tttttctgac atccattctg gagctatacc tacaactgga aggtcacaca
    22081 tatccatatc taagtaatta gctgctgctc caactaaatc tagtatacga cttatatcaa
    22141 cacaagaacc catatgtagt actggtggga ttcctactaa tttacagaca gttgcaagac
    22201 ctttacctgc atatttttca gccgcatcag attccattaa accaaactta gctgctgctg
    22261 aagctccaca acctgtagta actactatta tgtcgttttt gattaattct ttcattattg
    22321 ttacgtgagc ctcatttgaa actcctttag cattgttaca tccaactaca ccagctgctc
    22381 ctcttaatac tccagctttt agacagtctg ttaatggctt aactgttcct gctgggtcta
    22441 tatgagagtt tacaactctg tctaattgat ttactactgc ttctacacta tacccaactg
    22501 ttgcgcctga ttttaattca ggtataagta cttttgattt atctctattt ttaaagttta
    22561 aaatagcttc tttaactatt gctttagcat catcaagtgc ttgttcttct ctaaattcaa
    22621 tataagttga acctgttatc ttagcttttg gagaagttgt tacaaattta gtatggtaac
    22681 aatctgcaac tctagctaat gcagggaata tacattgaac gtcaactatt acagcttcaa
    22741 ctgctccagt tattattgca agttcttgtt gatggaagtc tcctgctatt tttacaccat
    22801 gtctcattgt aatctcatta cctgtacaac acatacctgc taagtttatt ccatcagcac
    22861 caacttcttt agctaaagct actaagtctg gctcttctga tgctaaaact atcatttctg
    22921 ataatgctgg ctcatgtcca tgtaatatta tgttaacttt attttcttct aatactgcta
    22981 aattagcctc tgttcttctt ggcactggag ttccaaataa gatatcgctt agacgagtac
    23041 ctatcataga accaccccaa ccatcagcca tagaagttct aagactcatg tgtattaaac
    23101 tatctatatc accagtacat cctatatgag tagagtgcat tattgttgca atttctctat
    23161 ctatagctct tggctctata gcgtattctt tccatatttt ttgtcttggt tctggtgcat
    23221 tttttataaa tcttaatgtc ccaaaaggtt ttccaaattc cattaatgca atttctgcca
    23281 cttcatgtgc tacatcatag atatctctac cttctgtctc tacatcccat tctttagcta
    23341 aagtaattaa cttagcttcg tctcttattt tatagtttcc atttggatca gctaatgcta
    23401 atgtatgtgc tatatctcta gcatggtctg aatgggcagc tgtcccacca gcaaccattc
    23461 ttgcaaagtt tctacctact atagtatgct catctgctcc acataacccc cttggagtct
    23521 tagggcttat tctacatggt cccattccac atattctaca gcaaacacct tgaagaccaa
    23581 aaccacattg tgccttaaag ccttcttttc tatcatacat tgtttctacg ccttcttctc
    23641 tagctttagc tatcatcttc tggctgttta aatcaattgt taacattttt tcatccatat
    23701 tcctatcccc tttttttgat ttttaaaatc ccccaggtga ctgacttcat gtcataaaat
    23761 aataaaataa atgctttcta agttgattat atactaattt tcagtgcttt gctttagatt
    23821 taaatatttt tttaacaaaa ttagataata cataaaaatt ttattttcta cacttaattt
    23881 aagttgattt tatcgcttgt ttttggctat ttgttaatat ctaaacctac ttttttgata
    23941 cttcagttta attaaattat gttttgattt ttttaaatca aatttgaaat ttttctagtc
    24001 atttttatat tattgcagat tgtttgtgta tttattatct ttcactactg tttggactta
    24061 ttacagattt ttactcccta aacttaatta gtaaaaattt cttagaatag cattttttgg
    24121 actaattttt ttgaattttt ttatttttcc tactttaata ttaattatag cttcaaattg
    24181 aacatttgta tatattcatt tgaagctata attatttttt atataaaact tttttaatac
    24241 agcattaatt tagacatttt gactattttt tctactttaa ttcatttatt tcaagcgatt
    24301 tatcctattt catattttta gctacaatag ccccatatgc aatagaatat aatttagatt
    24361 catgtgaatc agctcttgac actaaaacta taggagcttt tgcacccata attatacctg
    24421 ctgatttaga gtctgccata tatgtaaagg ctttccctat tccattgcca acctctattg
    24481 ttggaaccaa tatgatatct acatctccag atacttcact tttaaaccct tttatctcac
    24541 tagcttcttt tgaaacagct aaatcaaaag ccagaggacc ttctacaatt acattttccc
    24601 caaattctcc ttctttacaa gcttttgcta acatatctgc atctacagtt gcttgcatct
    24661 taggatttac tttctcttta gctgccaaac atgcaacttt tacagtttcc attcctaaag
    24721 cttttgcagc ttttatagaa ttttttaata tctttgcctt ttgttcataa tcaggagcta
    24781 tattcatacc tccatctgta gttataagta atttatggta tttttctaac tcatatacca
    24841 taacatgact taataaacta tcagttctaa gaccatagtc tttatttaac acttctttta
    24901 atatgacaga tgtatctaat aatcctttca ttacaaagtc ggcttcttta cttgatacta
    24961 atttaactgc tatttctgca cactctttga tatcatcctt atccactatt ttgataccac
    25021 ttaaatcaaa tcctatttct tttgatattt ctcttatttt gccttcattt ccaaccaata
    25081 taggagtaat tatatttctt tctacagcat cctttattgc cattaaaact tctttatcat
    25141 gagctgctgc tacagagagt acaattttct ctcctccctt aagttgttct aaaatatcat
    25201 ctaatttttt tatcaaaagt aacccctccc ttatatatat ttaactaaat aataccatat
    25261 ttgctatacc ttttctcaat ttatttttta tgttataaag aatttatatg actaaaagac
    25321 agtttaatat tttattctgg gatttcatca atagctctta aaagctttgc aggaacacca
    25381 cctacaatag tattttttgt tacaactgct ccagcagcga taattgcatt atctccaata
    25441 gtaactcctg gaactcttca tacaaaaaag aggcttatct ctattttgag atagccccac
    25501 ttaaccgata aaattaaata caacctaaca atatccttac ttatttattc caagttctct
    25561 tagacaagca atagtaattt catcttgtgg atttaattca taagctcttt ctatatagta
    25621 agttgcatca tcaatatcac cattatttaa ataagccatt cctaaattgc aaagtatttc
    25681 tgggtcttcc tttatcttag tcgcttgttc aaaatactct attgctttat ctaaattatt
    25741 cttataagcc tcacaaagcc ccaattccac tatagtatcc acctgattag gctttattat
    25801 caatatcttc tcaagataca tcttagcctg ttctatttcg cccataccct tatatccaag
    25861 tgctatcata aacagcaagt tccaccaatc catatgttct tcttctaatg gaagtaactt
    25921 ctctaatcct tccttaaact taccttggaa tacaagtgta tatccttctt catattgaac
    25981 tttaaagtcc atcttgccca gattctcttg tacctcagct actaaatcag catctaaccc
    26041 tagccttaat gcttcttccc aagtcagctt agtctttaaa tattgacctt gattataata
    26101 atggtagcct aagtgataat aacctaatgc aaaattctca tcaacatcaa ttaccttttc
    26161 aagcttagcc atggcttcaa gtaagaatgc attcattgcc ttatcatcca tatctttttg
    26221 atactgttta gctatctctt ggcaaacaat cgcatagtta tacattgcat ctaaatcatc
    26281 tggatacatt gttataaatg cttttatata tattaaagaa tctgtatgtt caccaatctc
    26341 aaagtatttt ctcgacatat atcccatata cgatttcaaa tccaaatcaa tatcttttga
    26401 taaagcattt aaaaacttct catactctgc attgttttta aacttactat ctattccaat
    26461 tatatatatc attgcatctg ctatagacat tgaatttaac ttatcttgag cagtcttttc
    26521 ttttatccct tttactaata cctcattttt tataggtaca tctaatccac cttttggcac
    26581 tttatagctt tttaatttaa actctccacc atgttttata gttataaaag ctaattcttc
    26641 tgcttttttt agcaaatatt tttctatctt aaaatccatt ttatcctcct aaatacttaa
    26701 atctctactt tatgagtttt agatttctca aatatttttt tatcatcata ctcaaataag
    26761 actacatttt ctacttctct tatttctgta tttatatcat tttttaaatc cacaacatta
    26821 aatctaaatg gctcaaaatg aacatatttt attatatgtt tagctggttt atcaacaaaa
    26881 ctaggtaaat atgttgataa gttatcatta tcttgtaaaa atacatggca tctattctta
    26941 aaatcatcca catctagctt attaaaaaac tgtggtatat ttgatgtttt acccagtgat
    27001 atatagtcat attttaatat atcattgaat acatcattat ctatgttgat tttttcatta
    27061 tagaaatcca tcaatatttt atataactga ttctttcctt gtgccaaatc aaagtagcca
    27121 ttttcatcaa aatacttggc aaaaacctca aagaatttaa aagggctttg tttataaaat
    27181 ctgcctataa tatatctcat agataacaca aagtttttag aattatagta tctctccagt
    27241 atatcttcta tatcctttag cttcaaagtt tcttcatagc ttatataatt attgtataaa
    27301 aactcatatg gagcataatc cttatattta aaaccatgct catctgcaac ttttctcata
    27361 cctgtccctt taatcatctt taaaaagcct aattgcagat gctctatacc tagattaaat
    27421 acatcattaa aagaattttc aaagctctta taatcttcat aaggcagtcc tgcaattaag
    27481 tctaagtgct gatgtatatt tctataagat gctacagttt gtacaacatg agacaacttt
    27541 ttaaaatcat cccttctacc tactgcatca agtgtttttt cattcgttga ctgaacccct
    27601 atttcaaatt gaaaaagacc ttctttacaa tcagccaaaa actcaagcat cttatcatct
    27661 atcaaatacg ctgtaacttc aaaatgataa gttgtatatc cattatcatt tttcataagg
    27721 aaatccatga tttccatagc cacttttcta tttgcattga atgtcctatc tataaacttt
    27781 atctgtgata ctcttgcatc tattaaagct tttaaatcac tctttatcct gtctaaacta
    27841 aaatacctta gacctggaat agtagatgat aaacagtact ggcagttaaa agggcaacct
    27901 cttgatgatt cataatacac tattctattt tcatattctt ttggattcaa gttttcataa
    27961 ggactaggaa tctcatccaa atcttgaagt aattccatag gcttattcac tattatctca
    28021 ttgccttgtc tatatacaag tccatttaca tcatttattt tcatctttcc ttgcaagtga
    28081 agtactaagt ctctaaaaat taattctccc tctccataaa gtatataatc cacaaaatca
    28141 tactttttca ttgcctcata actatcataa ctcacttccg gtccgcccaa agctatttta
    28201 gtattaggtc taattttttt tagattatca caaagtttaa ctatatctcc cacattccag
    28261 atatatgttg agaataatat tatatcatat tcatttttat atatatcctt taaaatatag
    28321 tccaaatcgt tgtttatcgt gtattctttc atattaactt ctatcaaatc tcttacaaat
    28381 tcttttagat atcttatagc caaatttgta tgaataaact tggaatttaa tgttgttaat
    28441 aaaattttca aatatttctc acctcttttt aagattatac tgtctttttg taccccttat
    28501 aggttaaaat agtatatgta aagtctaaat aatatctata taattaattt attaatttca
    28561 agtaatgtga ggaaaggaag ttttttatgg aattaattga aaaagtatct atcaactctt
    28621 tatcaaaaag agatttacta cttattataa agtctcttga atacactaat gaaaatactc
    28681 aaattagtga tttcattgac cttaggaata atatagtaaa ggaattatgt tttttaactg
    28741 acactaagga aaaagatttt ttagattatc tagaaaaaaa tagcacactc taataaccta
    28801 aaccttttta aactttaata tataaatacc tgatttagaa tttattttta tgcttaatat
    28861 attatcatta aaggttttcg acataactgt tatttgatta aatatagggt ctattatagt
    28921 attaccttct tcaagtctta taagacttct tgggtctatg cttttattat atgccttaac
    28981 tccaatttta tattttctca atttaacaac atattcggac atagaaaaaa taaaatctat
    29041 attatcttca tttaatttac tatcttcacc caaaatggat aatatagatt tttttatgct
    29101 attaattcct tcattttcta cagttctatc ttccatatat acatcatata gaacatttaa
    29161 aacatttaat aaaatacttt cttcatcata atcttcagat ttcaaatcaa tgtacttatc
    29221 aattaattgt attgctttaa tccttgccat ctgaaacttt acaacatctg atttgtctaa
    29281 ttctatagaa aatcctatta tcttgtcttt aatactctgt agtaattcga catactctat
    29341 tttactatta tttataagat tgtgcataat ggaattatac actacagaac ctaataaata
    29401 atcaaataaa agttcttctt ttttaaaata aataacatac ttttttatta gtttaataac
    29461 ctcatcttcg cttttttgaa tattagtatt tgataaaatt agtggtaata aaatatttaa
    29521 aaaattgata tttcctattt tttctccata aaatctatag taaataagtt catctctcag
    29581 tctattttcg tctatataca cacatctttg cgttttatca tctattaagg attttgtatt
    29641 tattatagtt aaatctttaa gagccctact tgcactacta aaagaagatg tgtagacttt
    29701 ttttagtaat tcgtcattca taaatatatc ctttctgaaa aattgaatgt aaataaattg
    29761 ttattaaaaa aaaagagaca gcccgtttaa gaagctgtca tatatagggg gatagtagtg
    29821 tacaaaaatc attgtaatct actatctata tattattata cgatactact tttttatatg
    29881 taaagcatca gttttcgaca tccctatatt ttacagttat acaaattgcg tactaagttc
    29941 aattttattc tttttatttc aacattttat catgctacat tctttccgga gcgtgcattc
    30001 ccaacaattt cagagaattt tctaaagttt gttttgttgc taaaactaat gcaagtcttg
    30061 cttttcttat ttccaaattt tctactaaaa ttggattgtc atgataaaat ttattaaacg
    30121 cttgagctaa atctaacata aatctagtca ctatatgtgg ctcatttttc tttagagcaa
    30181 gtaaaatatt cttattgaat gattctataa ctctaagaac ttctgcacta tcttcatcag
    30241 ctaatagact ataatctata tcagaagtca cttctacttc tgcttttctt aaaactgcac
    30301 agcatctagc atgtgtatac tgaacatatg gtccagtctc accatcaaaa ctaagcgttc
    30361 tttcccaaga gaaggtatag tctttaattc tactgttaga taactcttgg aatactactg
    30421 ccccaacacc tacttgctta gatatttcat ccacattttt agcatttggg tttttagcta
    30481 gtataatttc cttagtttta tctatagctt gatttaaagc atcttctaaa aatactactc
    30541 ttccttttct agtcgacata gtaccttctt caagtgcaac cataccaaat ccaacatgaa
    30601 ttaaatcttt tgcccaatca tatcccataa gctcaattac cttaaaccat tgttggaaat
    30661 gtaagttttg ttgagaacca actacatata tacacttgtc aaaatcataa gtctcttttc
    30721 tatatattgc agcagctaaa tctcttgtca tatataatgt tgaaccatca ttcttagtaa
    30781 taagtgctgg tggcatttta tactcttcta aatcaacaat tcttgctcct ttagactctt
    30841 ttaaaagatt tttctcttct aatagtttga taactctgtt cattttatca gaataaaaac
    30901 tctctcctgc taaagaatca aattctatat ctaaaagttt atacactctg ttaaactctt
    30961 ttaaactctc atttctaaac cattgccata attcttgtgc ttcttcgtca ccattttcta
    31021 atttattaaa ccatgctctt gcttcatctt ccatttctgg gtgttgttca gcttcatcat
    31081 ggaatcttac gtataatttt agtaattctg gaattggatt agactctact acttcttttt
    31141 ctccccactt tttaaaggcc actattaact taccaaactg agttccatag tcacctaaat
    31201 gatttattct tatagtcttg tatccttgcg aatcatatat cttatataaa gcattaccta
    31261 taactgtagt tcttatgtgc cctatatgga aaggttttgc tatatttgga gaagaatatt
    31321 caactattac agttttatct tttccaaaat cactatgtcc gtaattttct ttttcatcta
    31381 aaactttttt aattacagtt tctgctaatt gagatttatt tacaaagaaa tttacatatc
    31441 cgcctaattg tattactttt gttatctctc cacttggttc tatactttcc gccaattcac
    31501 ttgctatcat gtttggagct tttctaaaca ctttggctaa tttaaagcat ggaaaagcat
    31561 agtcccccat atccttattt ggtggaactt ctataagtgc ctctatctct tctttcgata
    31621 aatcttctat tttttcttta aggcaatttg atattgccac cttaaaatct tgcattctgt
    31681 tgctcctctc tacattgttc ctggtttata ttgtgcttat ctctttatgt taaatcaaat
    31741 ttcgtgtttt ttcaagtatt tcattacagt gtaaaacact ttaataatta tgttttctgt
    31801 atatattaaa aatactataa ttgcagtttt taatcacact attattgatt ttttataaga
    31861 cttttttcta ttttattgtt tcttgtacta tccagttgac tttatatact ttttttaatc
    31921 ttttatcaat tttcatacat caatattaat tttatataca tttattttgc tcttttatct
    31981 atttttatac atcaatatta gctttatata ctttttttac tcttttatct atttttatac
    32041 atcaatatta gctttatata ctttttttac tcttttatct atttttatac atcaatatta
    32101 gctttatata ctttttttac tcttttatct attttctata aatctttttc actcttaact
    32161 tttattcctt cttcttctaa cagccttgct gttatacctt tccctggtat ctttttacct
    32221 gaaaaactac catcatatat tagattacat ccacaagagg ggctaccctc ttttagtatt
    32281 gcttcgctac atacatataa atttgctatt tttagagttt cattagcccc tcttacaaag
    32341 ttttctgtta catccttatt atctttagta attacttttc catcaactat ctcagcaggt
    32401 ggtcttggtg tagatagacc acctaactgc tctgggcata caagtgtaaa ttctttgcct
    32461 tttaaatatt ccttgacttt ttcattttca ttattatcgc cactatattt acaatttatt
    32521 cctattaaac atgcagatac caatatcata aaaatgtgct cctatacaaa ttcttaatat
    32581 attcttatac aaattactaa tgtattctta tacaaattat tttaatttaa caattgttac
    32641 tcctgctcca ccttctccat attgaccacc tctttgtgat tttacatgtc tatttcttct
    32701 taaaatatct tgtagccctg cttttaatac tcctgtacct attccatgta ttactgttac
    32761 agactctaaa cctgccacat atgcatcatc aagatacttt tctacttcca ttatagcttc
    32821 ttctaggttt aaccctctaa ggtcaacttc attttttaca ctaccagatt ttgaccttat
    32881 aatgtttctc gttgatttag ttacatttgt tgaaacatct ttgcgtgttt tttgtaaaga
    32941 tttaaaaggt agagtcatct tcattatacc aatttgcact actgcttctt ttctcttttt
    33001 atctactgaa actacactac cattttgatt taaagttatt accttaactt cttcacctgg
    33061 ctttaagtct tttatctcct tatttgatac ctttggaact atcatacttt ttactgttgg
    33121 ctgaagactt cccatagagc tggtcaattc ttttctaagt tcttctatct tacgattctt
    33181 ttcttttgaa gctctttctt gttcaagatt tctaagttct tttattataa tatctacttc
    33241 ttctttagct tgtcttataa ttgaaaatgc ttctgattta gctttttcaa tcatcttatc
    33301 tctttgactg accaattttt caagtttttc gtcatactct actttcagtt tttcaatctc
    33361 ttcttttaat ctttcagctt cttctctatc ttctactgct ttgattctat tcttttcaac
    33421 attctgtaac acatcttcta aagctatgtt ttctgtattt atatattctt tagcacgtga
    33481 aatcacataa tcacttagcc caagttttct agaaatctca aacgcatttg attttccggg
    33541 aacacctata agtaacttat atgttgggct taaagtttct atatcaaatt caactgctgc
    33601 attttctaca cctggtttag taagagcata atttttaagc tcactatagt gagtggtagc
    33661 tatacattta gctcctacag aatttatatc ttctaatact gctattgcaa gggcagctcc
    33721 ttcaacaggg tctgttcctg ctcctaactc gtcaaatata actaaagagt ctgctgttac
    33781 gttttgtaat atagatacta tattcgtcat atgagatgaa aatgttgata gactctgttc
    33841 aatactctgt tcatctccta tatctgcaaa tacattatca tatacacaca tactacttcc
    33901 ataatcagca ggtatatgaa gtccactttg tgtcataagt gcaaatagac ctacagtttt
    33961 tattgtaaca gtcttaccac ctgtgtttgg acctgttatc accaatgtat ggaagtctct
    34021 tcctaaataa attgtatttg ctactacctt ctttttatcc agcagtggat gtcttccatt
    34081 ttttatgttt aaatatttat cctcatttaa agttggttct attcctctca tctgtataga
    34141 aagttttccc tttgaaaatg caaagtccaa tcttccaagt atctcttgat tagagattaa
    34201 atcttcgctc acttctccaa ccatagctga aatctctgat aaaattcttt ctatttcctc
    34261 ttgctctcct agtttcaact gcctcaattc attgttcatc tctactatag tcattggctc
    34321 tataaaaagt gtcgcaccag aagatgactg gtcatgcact ataccagcta cttgcgacct
    34381 atattcagat tttacaggta ctacaaatct atcacctctt agagaaatta ttgcatcttg
    34441 taagtacttt tgataagtag ttgaacttat tattgaattt aattttgacc taatggattg
    34501 atttttttgg gctattcttc ttctgatatc tctaagtgta cttgaagcat tgtcagatat
    34561 ttctacttca ctaattatag cattgtatat ctggtcttct atgtccttgt acacatatag
    34621 agaatttgat agagattgta ttattggata attaaaatct tcttcatcac tactagataa
    34681 gctatttctt aatcttctag ccactcttaa ggtatctgct atcataatca gacttcctgg
    34741 gtcaagagaa gcacctatat aagctctctt taccttatct tctatatcat gaataccttc
    34801 caaccctact gaacctctct ttataataat tgattgagct tcactagttt cttctagcat
    34861 agatttcact tctacaaaat cagtatttgg tactaaattt tctatatatt ttagacctaa
    34921 agaagatgag gctttcttct ttaacaaatc tattatttta ttgtattcta gaactcttaa
    34981 cgatttctca ttcattttct atattcacca tcctcgatat tttaactttt ttatttaatt
    35041 ttaaaaatgt atgttttcaa gcatttccaa taaaaaatgc aagttttatt tctttttcat
    35101 ttctaaaatt attatatttt ttctctttta gatatatttt a
//
LOCUS       DAEMHZ010000028        34756 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000028 DAEMHZ010000000
VERSION     DAEMHZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.028"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(175..831)
                     /locus_tag="KPK72_001261"
     CDS             complement(175..831)
                     /locus_tag="KPK72_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2488494.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKDTIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(886..1515)
                     /locus_tag="KPK72_001262"
     CDS             complement(886..1515)
                     /locus_tag="KPK72_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2488495.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1646..2287)
                     /locus_tag="KPK72_001263"
     CDS             complement(1646..2287)
                     /locus_tag="KPK72_001263"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG2488496.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2296..2967)
                     /locus_tag="KPK72_001264"
     CDS             complement(2296..2967)
                     /locus_tag="KPK72_001264"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2488497.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2972..3856)
                     /gene="rsgA"
                     /locus_tag="KPK72_001265"
     CDS             complement(2972..3856)
                     /gene="rsgA"
                     /locus_tag="KPK72_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2488498.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3954..4691)
                     /locus_tag="KPK72_001266"
     CDS             complement(3954..4691)
                     /locus_tag="KPK72_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2488499.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4799..6814)
                     /gene="pknB"
                     /locus_tag="KPK72_001267"
     CDS             complement(4799..6814)
                     /gene="pknB"
                     /locus_tag="KPK72_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG2488500.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6831..7583)
                     /locus_tag="KPK72_001268"
     CDS             complement(6831..7583)
                     /locus_tag="KPK72_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG2488501.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7603..8634)
                     /gene="rlmN"
                     /locus_tag="KPK72_001269"
     CDS             complement(7603..8634)
                     /gene="rlmN"
                     /locus_tag="KPK72_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG2488502.1"
                     /translation="MEKKKVVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8642..9967)
                     /gene="rsmB"
                     /locus_tag="KPK72_001270"
     CDS             complement(8642..9967)
                     /gene="rsmB"
                     /locus_tag="KPK72_001270"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG2488503.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMLEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(10029..10730)
                     /locus_tag="KPK72_001271"
     CDS             complement(10029..10730)
                     /locus_tag="KPK72_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG2488504.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10799..11551)
                     /locus_tag="KPK72_001272"
     CDS             complement(10799..11551)
                     /locus_tag="KPK72_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG2488505.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKTPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(11561..12490)
                     /locus_tag="KPK72_001273"
     CDS             complement(11561..12490)
                     /locus_tag="KPK72_001273"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG2488506.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12505..12945)
                     /gene="def"
                     /locus_tag="KPK72_001274"
     CDS             complement(12505..12945)
                     /gene="def"
                     /locus_tag="KPK72_001274"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2488507.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12963..15488)
                     /gene="priA"
                     /locus_tag="KPK72_001275"
     CDS             complement(12963..15488)
                     /gene="priA"
                     /locus_tag="KPK72_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG2488508.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLKKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15727..16926)
                     /gene="coaBC"
                     /locus_tag="KPK72_001276"
     CDS             complement(15727..16926)
                     /gene="coaBC"
                     /locus_tag="KPK72_001276"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG2488509.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16931..17197)
                     /locus_tag="KPK72_001277"
     CDS             complement(16931..17197)
                     /locus_tag="KPK72_001277"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG2488510.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17200..17817)
                     /gene="gmk"
                     /locus_tag="KPK72_001278"
     CDS             complement(17200..17817)
                     /gene="gmk"
                     /locus_tag="KPK72_001278"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG2488511.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17847..18728)
                     /locus_tag="KPK72_001279"
     CDS             complement(17847..18728)
                     /locus_tag="KPK72_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG2488512.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18732..19562)
                     /locus_tag="KPK72_001280"
     CDS             complement(18732..19562)
                     /locus_tag="KPK72_001280"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG2488513.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(19849..20883)
                     /gene="ltaE"
                     /locus_tag="KPK72_001281"
     CDS             complement(19849..20883)
                     /gene="ltaE"
                     /locus_tag="KPK72_001281"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG2488514.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLRELIGVC"
     gene            21179..22954
                     /locus_tag="KPK72_001282"
     CDS             21179..22954
                     /locus_tag="KPK72_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG2488515.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23169..24821)
                     /locus_tag="KPK72_001283"
     CDS             complement(23169..24821)
                     /locus_tag="KPK72_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2488516.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCGHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25120..26331)
                     /locus_tag="KPK72_001284"
     CDS             complement(25120..26331)
                     /locus_tag="KPK72_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG2488517.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26615..27148)
                     /gene="pyrR"
                     /locus_tag="KPK72_001285"
     CDS             complement(26615..27148)
                     /gene="pyrR"
                     /locus_tag="KPK72_001285"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG2488518.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27508..28455)
                     /locus_tag="KPK72_001286"
     CDS             complement(27508..28455)
                     /locus_tag="KPK72_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2488519.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIQIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28424..28870)
                     /gene="lspA"
                     /locus_tag="KPK72_001287"
     CDS             complement(28424..28870)
                     /gene="lspA"
                     /locus_tag="KPK72_001287"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2488520.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29180..29959)
                     /locus_tag="KPK72_001288"
     CDS             complement(29180..29959)
                     /locus_tag="KPK72_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2488521.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(30014..30496)
                     /locus_tag="KPK72_001289"
     CDS             complement(30014..30496)
                     /locus_tag="KPK72_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2488522.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30762..32183)
                     /locus_tag="KPK72_001290"
     CDS             complement(30762..32183)
                     /locus_tag="KPK72_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2488523.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32329..33099)
                     /locus_tag="KPK72_001291"
     CDS             complement(32329..33099)
                     /locus_tag="KPK72_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488524.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(33104..34306)
                     /locus_tag="KPK72_001292"
     CDS             complement(33104..34306)
                     /locus_tag="KPK72_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2488525.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11066 a         6377 c         3494 g        13819 t
ORIGIN      
        1 tttaaatata tttaaaatta atatacaaaa aaaagtagtt cttattgtat taattaaatc
       61 aatgatttga aatataaaat taatatcaat aaaacctact ttttaatttt ttaaatattt
      121 atttttaatt tattttgttt ttaatttact tcgattttaa aatgtccttg acttttattt
      181 tataaatgta catatttctt ctactggttt tttagatgga ctcttcaaat tgtcctctgg
      241 cactccaaga gctaacattg cgcctaaaaa atactctccc ttttcaaaac cagtttcggc
      301 ttctaaaaca gcttctatct catctgctgc atagttttga cttgtcaacc aacaagaacc
      361 atatccaagt tcaattgctg atagtgtaaa gttttcaatt gcagcaccta gactctgcat
      421 acctggattt cttataaata atttatctat agtatcctta ggtgcatcta taagctctaa
      481 ttcataatat cctgatgggt tatatacctt tgtaaaaact aatactaaaa ctggagcttt
      541 taagtaaaat agtgtaaagt ttttaacaaa ttttctaaat ctattagcct tatccactga
      601 tactttatcc atttctacaa gtatttcttg ttgcttttta gttatcacat ctgctatctt
      661 ttccattaaa tcacggcgtt ttataacaac aaaatgccag ttttgaatat tcttgcctga
      721 tggtgctgca cctgctgctt ttatcatttt taaaatatct tcatctgata catcttgatt
      781 tttaaacttt cttacacttt gtcttttaaa aatagtatct tgtaattcca tttttaaatc
      841 cccccataac ttttttatta ttttaatata atttttaaac tcatcttatt ttaatatatc
      901 atgttctaac aaatctttaa atgaatctat aaaataatca tatacatcta cattcccaaa
      961 cccatatttg gcatatataa aaggtatacc tacaaactta gcactatttg catcaccttg
     1021 cgtatcacca acatagattg gatttattaa tttatttctt tcaattataa gtttattatt
     1081 ttctccttta tgcaaaccag tattaccagg acattcaaaa tctataaaat actgttctag
     1141 tttatgtgct tttaaaaaag attctatata tccagcttga caattactta ctataaataa
     1201 tttatatttt ttagatagta atttaagagt atcttctata tcatcaaata atttagctcc
     1261 attttcagat aaatagtcac attctttcaa agaacattca ttcaataaat tcattctttc
     1321 ttcttcactt aaatttggaa ataaacgatt tgctatttcc tcaaaagaaa gccccataag
     1381 acttctaaat aaatcttttg tgacacttaa ttctatttcc ttatgtttac ttaaaacatc
     1441 ctgccagact ttacatactt cttcagttga atcccacaat gttccatcta aatcaaaaat
     1501 aatactatct accacaaaaa atccccctta tgtcaaagtt tattatatat ttataattat
     1561 atactaaaaa gcagatacaa tcataaatat attttacttg attgtatctg ctctacaata
     1621 taaaaccaat tgtcaaaatc aaattttata aatttctaac tacaagtaca cagccttgct
     1681 ctactttaat cttacaagaa ttactcaaca taacatttga tattccaata ggtacagagt
     1741 atttcatata ataattatcg agagggtatt ctaagccagt aagtgttaca cccttagcat
     1801 caccatttat agctattaca gaaatagtgt cccctgggtt tccatacaat gaaatttcct
     1861 cattttcaac tatatacatc tcttctttat cagaaagtat cctagaatat ataccatctt
     1921 cttttaaata atataatagc tttatatttg caagagtgtg gtcaatcctt ccccctaaag
     1981 caccaaaaaa atcaatatga tttgctttaa gtgtcttagc taaaaaaaga caaagctctg
     2041 tatcagtctc atctttttta gatggaaatt tttcaaattt aacaccttta tttttataga
     2101 agtttatctt ttcttcttct actgaatcta aatctcctaa tatataatct ggcattattt
     2161 ccattttata agtgtgattt gcccctccat ctgcacatat tatgcagtca taacattcct
     2221 taattataat atctcttgta acttcgtaat cttcaatttc gccatttaaa acaatacaga
     2281 ttttcattat taaatttaaa ctaattcttc aagagaagca ttctttctaa ataaattaac
     2341 tgtttcttga atgtcatcac ttccaaatat tgcagaacct gctactatta cattagctcc
     2401 tgcttgtaca acttcggcta cgtttttagg gcttatacca ccatcaactt ctatatcaat
     2461 gtttaatcct tgactatcta taatagcttt taactcttta atcttatcta aaacacaagg
     2521 aataaatgat tgtcctccaa atccagggtt aacagacatt aataatacca tatctaaatc
     2581 agatagtaca tgtttaatcg tatctactgg tgtagctgga ttaagtacta caccagcttt
     2641 tattccatgt gattttatat tttgtatagt tctatgtaga tgaacacaag cctcctggtg
     2701 aacagttatt atatcacatc ctgcttttac aaattcttca atatactggt ctggattttc
     2761 tatcataaga tgagcatcaa ataccatatt tatattttct ttctttaaac ttttgactac
     2821 aagtggtcct aatgtaatgt ttggtacaaa gtgtccatcc attacatcga tatgaagata
     2881 ttcacatcct gcactttcta cttttcttac atcttctaat aattttgcaa aatctgctga
     2941 taatattgat ggtgctaatt taatcatgtt tctaatacct cctactatta ttttgtctta
     3001 tttcatgtag cagctgtata taactatcat atctttcttt agatatttct ccatttgtaa
     3061 cagcttcttt tatagcacaa ttaggttcat tttcatgaat acactttgaa ccaaacttac
     3121 aatcattaaa tttatcaaat tctataaaat aatctttaag ttcaacttcc tctatatctt
     3181 caagtgcaag ggaactaaaa cctggtgtat ctgcaaccat tccaccaaat tctaatttta
     3241 aaagttcagc gtgacgagta gtatgttttc ccctttttat tttatcactt acaactcctg
     3301 tttgaagttt aaaattttca tctatttcat ttaataagct tgattttcca actccagatg
     3361 gtcctgcaaa cactactaca ttttctttta attcttcttt tactttatct atatttaact
     3421 tagtaatatt acttacagga atgactttat agccactaag ttcatatata tttttaacag
     3481 tttctaatgt atcattatca tctaaatctg cttttgtaag gattattaca gtttctaaat
     3541 tttctttttc tgccaaaact ataaatctat ctaaaagaga taagtttggt ttaggatttt
     3601 ttattgcgaa tactattaat gctttatcta catttgcaat aggaggtctt ataagttctg
     3661 tatctctact gtcgatttct tctaagatac cctttttttc atcttcatca acaatgctta
     3721 ttttgactct atcaccaacc aaaggtgtta ttttctgctt tctaaatatc cctctagcct
     3781 tacattcata aataccatta taagtatcta cataataaaa tccactaatc ccctttataa
     3841 tttttccttc tagcatcaaa tgcctccctt tataaaattt atataaacac atactgttta
     3901 tgagtagtat atcgtgttat tctttcctat ttttatataa taccacattt tttttaaata
     3961 acaaatcctc cgtttggact aattacctga ccagtaataa aatttgattt gtctgatgcc
     4021 aagaaaatag cacaatttgc tatatcttct ggagttccta gcctcattag aggggtttct
     4081 tccacaagag cgtctatatc ttcctcatta tatccagata acatgtcagt attaatgaca
     4141 cctggagcta tgctatttac tctaatattt gatggtccta cttctttagc taatgccttt
     4201 gtcataccta ttatgcctgc ttttgttata gaataatgca cttcacaaga cgcacctgat
     4261 ataccccaga ttgaagatat attaattata tttccttttt tttctgatat catgtatttt
     4321 aaagcaactt gtgaacaata aaagcttcct tttaaattaa tattcatcat attatcccaa
     4381 tcttcatcag ttatatctgt aaacaactta tcttgactta caccagcatt gtttacaagt
     4441 acatccaatc ctccaaattc ttttatacaa taatctacca tatcctctac atcttctcta
     4501 tttgaaatat cagccttaaa aagttttaca gaaaaatttt tttcatttaa tatagtgtac
     4561 aactctttag cttcattttc tgacttatta aaatttacta atacattgta accttctttt
     4621 gcaaaagctt tagacatagc tttaccaatc cctctagctc ctcctgtaat taaaactgtt
     4681 ttcttcttca taaatacttt tctccttata ttataaccaa ttcagtattt tatacaaaac
     4741 aaactgccct ttaaagaaaa tattttctat aaaaggacag ctcttatttt attaatattt
     4801 attctgtttt accaatgttt gcgccagttc ctgatgaagt tcctccatta tctcctgagt
     4861 ttgagcctcc tgaactatct ccattgtctc ctgagtttga acctcctgaa cttcctccat
     4921 tgtctcctga gtttgagcct cctgaacttc ctccattatc tcctgagttt gaacctcctg
     4981 agtttccacc actatttcct gagtttgaac ctcctgagtt acttccatta ttgcctgagt
     5041 ttccaccact atttcctgaa tttgagcctc ctgaatttcc accactgttt cctgagtttg
     5101 aaccaggttc tgttggtgta ttattctctg gtgttgttgg ttttttatct ggtactgttg
     5161 gagtctgtgt atttgatttt tctgaacctt tgcttacata taggtttacc tcatcacctt
     5221 cctgagctct tgatgagcca gcacctggac tctgacttaa aactgtacct tctttatata
     5281 ttgagctgta ttcatactta acagttccca cttttaagtt attttcttca ataagttgtt
     5341 ttgcattact caaagtaagt cccactacat taggaacaga tgcctgactt gaacctttac
     5401 ttacgacaac atttatagta tcaccctttt tgacatttgt acttccacct tcaggtgtct
     5461 gtgatattat acaattttca tcaacttcac tattatactc ttcacttttt acactcaggt
     5521 ataatccttc tttttcaaca gcactttgag cttcttctaa tgtcatattt acaagattag
     5581 gaacagtcaa agatttattg ccaaaccctc cagcaaataa aaacttatat gctaagaata
     5641 cttgagcaca caatatcagt atcaaaacag ccgcaattac ttttaatctt cttctagatt
     5701 ttgggctttc ctgttttttc ttctttttct tagttctctt acttggagta ctcttaggtc
     5761 tctgattctt tttagctctc attatctctt cctcttcttc atcttcatca tcatcttctt
     5821 cataaaaatc atcatagtaa tcttcatcat catccaaatc agctacttca acaggcttaa
     5881 ccactttttc aggagctggt tttgcaagtg ttggatttac aactttatta atttcttttt
     5941 catcaatttt cttagttgca aaatcatcat attcttttat aaaatctaaa tcaatatttt
     6001 tttctatata ctctatatct tctatcaatt cttcagcagt ttgatatcta tctgcactag
     6061 atttttctgt aagctttttt attgtagttc ttacactttg aggaatccta actttttctt
     6121 ctgatgtaaa gtctatatca tcattaatgt gttgaagtgc tatagaaatt ggactatctc
     6181 ccctaaatgg aacttttcct atcaacattt catataaaac tatacctaga gaatacaggt
     6241 cagcattatt agttacaaat ttacctttag cttgctctgg tgaaaaataa tgcactgaac
     6301 ctattatact accaatatta gtcattgttg aatttgaaac agctttggct ataccaaaat
     6361 cagccacttt tactactctt ccttcatttg atatgagaat attatgaggt tttatatctc
     6421 tatgtataat cccctttttg tgcgctgcac taagtgccat agctatttgt tttgttatat
     6481 ctaatgcagt atattcatct aatatacctt catcttgtat aatctctttt agattcttcc
     6541 catctacaaa ctccattaca atgtagtgaa cttttccatc ttcaccaaca tcataaacat
     6601 ttactatatt tggatgtgaa agacttgcta ctgcttctgc ctctctttta aactttgtta
     6661 gaaattcatc atcatctaca aattcaggtc taagtacttt aagtgcaaca gtcctgttta
     6721 ataatctgtc tttagcctca tatacaaagg ccattcctcc atcaccaatc ttcctgatga
     6781 tttcatatcg atttcctaaa attgtatctc ccacaatatc accgtcctaa ttatactttt
     6841 atcactataa ccgaaacatt atctttacca gaaacatcat tggccatact tatcaattct
     6901 tcacttatat tactagttct ctcatattca taatctaata ttaaatctct tatatcttca
     6961 tcatcaataa acccagttaa tccatctgtc gcaagaagta ttatatcact ttttttgatt
     7021 tctttcttaa aaatatctac aacaaccata tcatctgttc caattgctct agttattcta
     7081 ttcctctgtg gatgtcttct tgcctcttct tctgtaatga catgggccat cataagttct
     7141 tcaaccacgg aatggtctat agttatttta tcaaattttt catctgttaa caaataacat
     7201 ctgctatctc caacatttgc cacatataag acattattat acactatagc cgtaactaat
     7261 gtagttccca ttccttcaaa ttctatatct tccatagatt ttttgtgcac aattgagttt
     7321 acattattat aagcctgttt taatatatca tctatatagt ctatttttac attatcatgt
     7381 tgtaataagt tttcctttag aaaatcaatt atattttcta ctgctaattt actggcgact
     7441 tcaccttttt tatgtccacc cattccatca gctatggcaa atattcctat tggaccatgt
     7501 tctgtatcta taacttctcc ctcacaatag tcttcattgt tttttcttat ttttcctata
     7561 tgggaggcac aactataaac catacgactt cctccttaca aattacttgt attttcttct
     7621 caattgccca caagctccac taatatcaga acccatagac atcctaactg tggcaggtat
     7681 attgttcttc tccaaactat ccctaaattt ataaataaat gctttatctg gcttttcgta
     7741 ttctctttct tcaactttat ttattggaat taaatttaca tgacatagca taccttttag
     7801 gagcttagct aatgcctttg cttctttttc tgagtcattt acccctttta taagagaata
     7861 ttcaaatgta acccttctat ttgtcttttt tatataatat ctacatgcat tcaatatttc
     7921 ttcaatagaa taagcatttg ctacaggcat aatttttctt cgttcttcat catatggtga
     7981 atgaagtgat aaagctaaat ttatagctat ttctaagtct gccaactcat acattttagg
     8041 tactattcca caagttgaaa gagttatatg tctataccct atattaagac catttttttc
     8101 gttaattatt tttaaaaatt gttttgtatt ttcaaagtta tcaagtggct caccactccc
     8161 catcaataca agatttgata ccctcttacc agtatcttct tgaattttaa taacttggtc
     8221 taaaatttcc catggctcta agtttctaat taatccatcc attgtagatg cacaaaaatt
     8281 acatcccatc ctacaaccta cttggttaga aacacagaca gtaactctac tatcataatc
     8341 catcatcaca gtttctatta tatttccatc atctaataaa aacaaatatt tttttgtatt
     8401 atctacctta gattctaact ttaattctat atctatatgc cctatacatg aaacctcttc
     8461 taatttattt ctcaaacttt ttggtatatt gttcatatca tcaaaagttt ttgcaccctt
     8521 atatatccaa gagaatattt gacttcctct aaagggcttt tcatctatag ttttcataaa
     8581 ttctttaagt tcatcttcag taaaattttt taatactacc tttttctttt ccattctctc
     8641 actaccttat tctctttaat tttgctatga aaaaaccatc tataccatga atattaggat
     8701 atatctttaa atatccttta tcttgattgt ctaagtctac atttactgta tcaattggaa
     8761 caaattcaaa gttgtgattt tcttcaacaa aagaagtaac tatatttata ttctccacat
     8821 cttgaattgt acatgtacta tatactaaag ttccaccaac tttgacatat ttagatgcat
     8881 tttctaaaat tttcttttgt atagaagtga tgtcttttaa ctcttcttct ttcttatact
     8941 taatttctgg tttacgtctt attataccaa aaccagagca aggaacatct gctaaaacat
     9001 aatcaaattt acttatacta ttttcatcaa tctcagaagc atcaaatctt tctacaaaca
     9061 catttttaag ccccaatcta tttacagtag agtttataag ctttagttta tgctcaaaaa
     9121 tatctctagc tactacttga ccagtattgt tcattaaagt agctaagtga gtagatttcc
     9181 ctccaggagc actacataca tccaaaacaa atgaaccttc ttttggatta attactttcc
     9241 ctactatcat agaacttata tcttgaattg taaataaacc agccttaaat aattcatttt
     9301 tttctatatt tttcattttt tcgactttaa tagcttcttc tagcatagga acttttaaac
     9361 atacaatacc catttcactt aatttttcaa taagttcttc tctacttatt ttaagtgtat
     9421 tcgttcttat atagatacta ggcttttcat tatttgcttc caacaagtct tcagcaaatt
     9481 cttgtccaaa tttatctatc cagtttttta taatccaaga gttataggaa tactttatag
     9541 ataagtattt tacactatct tcctcagtta tgtccattat agtctcttta gctcttattt
     9601 catttctaag aatagcattt atgaaaccag aagatttttt atcatatttc ttagataatt
     9661 ttacagtttc attaactgct gcataatctg atatactatc taaaaataaa atctgatatg
     9721 ttcccattct taaaaagatt ttaacataag ttgacatttt ttttaccttt atttttgaaa
     9781 gtttattgat tatataatct aaataatatt tattttctat tactccataa attaactctg
     9841 ttgcaagtcc tctatccata tcacttattt ctaaattttt aaaatgctta tttattgcaa
     9901 tatttgaata attgtcattt ttttctatat cacataatac tttaaatcca acttctcttg
     9961 catccatata aatttctccc ttttattaaa cataaaaaag ggttgtttct aaacaaccca
    10021 ctttactttt agtcatttct tctttgagct attagtaaca atctaagtaa ttgaagtatt
    10081 gaaaccaatg ctgctgcaac atatgttaaa gctgctgctg aaagtactct tctactttgt
    10141 cttgactcac tttcatctat aatccctaaa ttcccaagtt gaacgattgc tctacttgaa
    10201 gcattaaatt ctacaggcaa tgttattatt tggaataaca ctgaagccga aaacaatagt
    10261 ataccaattt ttaaaaatgg accagccata ataaatccta aaaatattaa aaaccaagat
    10321 atacttgaag caaaattcac tactggtact aaactacttc ttatttgaag aggtgcataa
    10381 cctttagcat gttgtatggc atgaccacac tcatgtgcag ctactgcaac agaagttata
    10441 gatgttccat aatatacatc ttctgacaac cttacagcct ttcttcttgg gtcataatgg
    10501 tcacttagat gccctctaac catttctatc tttacatcat acaaaccatt tgaatcaagt
    10561 actcttcttg ctgtttgctc tccagtatat cctcgacgtg tatttactct taaatactta
    10621 tttgttgttg aactcacttt aaattgtgca tatatagtaa gtaatatagc tggaattaat
    10681 acaaccattg ttgggtcaaa tccccaaaaa ccgccatata cagggtacat aaataacact
    10741 ctcctaaaat atatttttat tatacatcct cttaatattc acttaactta aaaacagttt
    10801 aattacaaac taatatattt tttcctgtta aaaagcctat agctttttca atatccatca
    10861 tgtctacatt agtatttaca caaggtccat taggtctttt gttaaataca cctaaaacag
    10921 gagttttctt aatatcttgt attccagatg tcaaatctct ttcacatgca acagctacaa
    10981 cagctttagg tttattatcc attataattt ttctagctag ggttcctcca gtagctacaa
    11041 atatttttac attagttttt tcctttagct taattaattc tcctacgttg catcttccac
    11101 attctttaca attatctata tcattagtaa cttttaattt acaactattc tcttgaatac
    11161 aatgaggtat taaaattatt atatcctctg gattaaaatt atatttatta ctgtatatat
    11221 attcattatt taactttata tatattcttc taatatcact ttttgacata ccaaatgatg
    11281 atgcaatgaa agatataata ggaaataatc cactgacaat attaaaattt attttcatta
    11341 tatttgagtt aacatacttt cctttaatta ctctataagt aactgctgtt gaacatagta
    11401 ttataagtat tagtgatatt attataatat ttatcatcaa ggagaatatt tgagttaaac
    11461 ttattataaa aaaattaaca actcctatca aaattcctaa tattaaaatc aaaatcccaa
    11521 cacttgttaa atacttcttt atatctgtca tacttttcac ctaacccaat actaaaccag
    11581 tttctatagt attccctttt atatattcac caaccaacat tctcttttta ttcggcatct
    11641 gaatttcttc aattaaaagt acattatcta tagtacttac ttttatccca tctttgctta
    11701 catcaattat tgtacctaca tcttttgtgc ttttttcatc taacacttta gttttccaaa
    11761 ctttcattat gacatcatta taggtagtat aagcacttgg ccatggattt actcctctaa
    11821 ctaaattgtg tatttctctg gcactttttg aaaaatctat gttacctaat gatttattca
    11881 ttattggcgc ataggaaaat tcttcatgat tttgtacttc tcttggagca tttccttctt
    11941 caattaatct taatgtttct tttaaagttt ctgctcctat attcatcatt ttatcatgaa
    12001 gttctcctgc tgttatattt tcatcaagat ttacttctgt ctttagtatc atatctccag
    12061 tatctagccc ttcatccata tacatagttg taacaccagt cttttcttca ccatttatta
    12121 ttacccaatt tataggtgcc gcacctctat attttggaag taaagaaaca tgaacattta
    12181 tacatccaag tttaggaatc tctaatattt cttttggaag tatctgtcca aaagctacaa
    12241 ctactattac atctggattt aaagatttaa ttttatctat aaattcttta tctctagcct
    12301 ttactggttg atatacaggt atattatttt caattgccag ttcttttact ggactcatac
    12361 ctagtttttt acctcttcct tttggtttat ctggttgagt tacaacaccc aatatttcat
    12421 acttctcatc tattattttt tgtaaacatg gaactgctat atcaggagtt cccataaata
    12481 ctattttcat caaatcaact cctactactt ttctattttg tcaacaaaca aaattccatc
    12541 taagtggtct atctcatgac aaaatgctct agctaaaagt tcttctccct ctaactctat
    12601 tgtttcacca tttctattta aagctctgac ctttacataa tttggtcttc taacatttcc
    12661 cgcttctcca acaacactta aacatccttc ctcatctatt tgctccccag atgtttctat
    12721 tatttcagga tttataagtt ctataagctc ttctccaata tctattacta ctactctttt
    12781 taatattcca acttgaggtg cagccagtcc aacaccatct gcatcataca tagtttctgc
    12841 catatcatct aataattgta ttatcttttc atcaattttt tcaacctttt ttgatttttt
    12901 tcttaatact ggttctccta tttgaactat ttgtcttaaa gccattataa ttcctcctaa
    12961 atttataata cactgtttgg attaatatct atagatacat tgatattctt atcaaatatc
    13021 aaatctcgtt ttgttataca tatatatttt attataccct ttaagagatt aatttcaata
    13081 ttttcatctt taaagagtat ttgccatcta taattttgat ttatttttga tattgaacat
    13141 ggatttggac ctaatataaa tcctaaatca tttatacccc ttttttctaa taaataaatc
    13201 aatgatgcat gcatattttt tatatttttt atcactaatc tttcatcttc accactaaca
    13261 actacactta acatattatt aaaaggagaa tatccaaatg cttttcttat ttttatctca
    13321 tcttcataaa aaccctcata atcatattca atagcatgct taatagcata gtgttcagtg
    13381 tcataagtct gaagtactac ttttccctct ttatcagctc tccctgctct tccagagact
    13441 tgagttatca attgaaatgt agtttcaaaa cttttaaagt ctggaaaatt taatatcatg
    13501 tctgcagaca aaatacctac taaagttaca ttctcaaaat ccagcccttt acttaacatc
    13561 tgtgttccaa taagtacatc tgcttcttta tctttaaatt tatttagtat ctcatctaaa
    13621 gcaccttttt ttgaagttgt atctttatcc attctcaaag tttttatatc tggaaaaata
    13681 tattttaatt cctcttcaat tttttgagtt ccaactccaa atggcttaac ataagtactt
    13741 ttacattcag gacactcttt tggaatttct ttctcatatc cacaatagtg gcatcttcca
    13801 gtattgcttt tcttatgata agttaaagat atatcacaat tttcacattg aaacacatat
    13861 ccacactttc tacaagatac aaaatttgca taacctcttc tgtttaaaaa tagtattact
    13921 tgattatttt cttcaatcgc ataacgtatt tcttttttta atttcaaact aaatatactt
    13981 ctatttcctt tatctaactc atctttcatg tctacaactt caatgtttgg aagaggctta
    14041 tcatttgctc tactttttat atttataagt ttgtattctc cagattttgc cctataatac
    14101 tcttcaattg agggcgttgc tgaacctaaa ataagagtta tattattttt taatgccata
    14161 aatctagcaa cttctatagc attaaatttt ggattcttct cagacttata tgatgactca
    14221 tgacattcat caattattat taagcctaag gaattaaaag gtgcaaataa agctgaccta
    14281 gctccaatta acactcttac tttacctgct ttgacagctt tgtaaacatc atgtttttct
    14341 ccttcagata gtttactgtg gaacactcca actaaatctc caaacctact ctttagccta
    14401 tctattgttt gaggagttaa tgctatctca ggtactaaga atatactgtc taagccttga
    14461 tttagagcaa attctattat ttccatgtaa acttcagttt ttccacttcc agttactcca
    14521 tgaattaagt atggtttttt atcatctaca aacatattcg actttatctc atcaactgct
    14581 tcttgttgct cattgtttaa ttttatactt tttagtacgg atttgtaaat actttttggc
    14641 tctctataat aatccttaaa ttctagggtt attagctttt tcttacttag agaatttata
    14701 ctttgttttg aagcatttaa taaatccaat aaatcattta tctctacatt ttcattattt
    14761 ttaagaaaac ttaatatctc tttttgttta cttcccacat ttattttatt ttgctctata
    14821 taatcgtcaa tcttatcact ttctaaactc aaagatatat aacatacttt cttttcattt
    14881 ttgtgatttt tatattccca acataatttt ataattccat ttttattcat tgtgtacaaa
    14941 gaactattta agtttggtat atattttaac ttatcaacct taattttacc tttactatca
    15001 atcactttct ctactatttc tctattttta tcacttaaag ttgatatttt ttccttcaat
    15061 tcaatatcat ttaatccact taaactttca cctaatagga ctactttata attatttaat
    15121 ttgtatccct ttggatatat caaatttata cattctatat aagtacacaa atatctattt
    15181 ttcatccaat aaaccaactc taaatcttct tttctaaata ttggattttc atctaatata
    15241 tcaataattt cttttatttt tattttttca tctaagctat ctgtaaattg aaatacaaag
    15301 gcttctgttg gtttattacc ttttccaaat ggtactaata ttctgtgccc gatttttatt
    15361 acatcagata aaaatacagg aatttgatat gtaaataaat tatctgtata tatagtatta
    15421 cttctaacta ttacctttgc atattttttc attataaaac ctctacttaa atttaatata
    15481 ttgttcatat tttaatatac tttacaagat attaaaattt cagaatttat aaacaatttc
    15541 aagttattaa tattaacttt attttaataa ttaactatta attatatttt atactttatg
    15601 acaaaataaa tgctgaaaac tacaatttaa ctgacatttg cgagttttta ctcgctaaaa
    15661 ttaaattgca gctttcagcc actttagctg tgataaaata cacaaatatc atatttaaaa
    15721 aaggtcttat ttctcaagca acgctttaac tttatctaaa ataacattgg ctacttcttc
    15781 ttttttcatc ttaggatact cagtaatatt tccctctaca tctattattt ttacaatatt
    15841 agtatcagct ctaaaaccag caccttcttt agttaaatca tttgccacaa taaaatctaa
    15901 atttttcttt tttatttttc ctttggcatg ttctataagg tcattagttt cagctgcaaa
    15961 tccaattagt atcttatctt cttttatttt tcctatttca taagcaatgt cataatttct
    16021 acttagttct attgataaat catcattctt ctttttaatt tttttgtcag aataagtttt
    16081 tggtttataa tctgcaactg ctgcactttg aattactatt ttattttcac ttaaatttga
    16141 tactactgct tcatacaaat cttgtgctga ctctatttga acaaattttt taagattttg
    16201 tggaatagaa agatttgtag gacctgatat taatgttaca tcagcacctc tagctatagc
    16261 ttctcttgct atagaatatc ccattttacc agaagaccta ttagttaaat atcttactgg
    16321 gtctatactc tctacagttg gaccagctgt aactatcata ctttgacctt ttaaatcttg
    16381 ctctttagta agattagtca aaacttcctc tactattata tctggattag ctagtttacc
    16441 tgttccaata tctccacatg caagccttcc actttccggt tcaataaatt tatatccaaa
    16501 gctctttaaa gtttgtatat ttttttgaac aattggattt tcatacatat tagtattcat
    16561 agctggtgct ataagaacct gtgcttttgt tgccataaca gtagttgtca acatatcgtc
    16621 agctatacca tttgcaatct tgcctataac atttgcactt gctggcacta ttaagaataa
    16681 atctgctttt tttgctaatg atatatgttc aacttcccaa gtttttggtt cttcaaacat
    16741 atcttctact acataatttt gactcagcga ttgaaatgta agtggtttaa caaattctgt
    16801 tgctgatttt gtcattataa catggatatt tgcatttagt ttttttaact tacttacaac
    16861 atcacaagcc ttatatgcag ctataccgcc acttatgcca attacaacag ttttatcttt
    16921 tagcatgacc ttactcctct tctgaaattt gttggtgttg ctcagcatga tgtctagctt
    16981 cttctatctc tatttcctct tctgtcaata atctatatgt tatttttcca cttgcaactt
    17041 ctttagttgc tacacataca ggcttttcct tagttttatt tgaaacatat ggttcttctc
    17101 catcaattaa ttttcttgct ctctttgaaa cagtccctac taaatagtat ctgttgtcta
    17161 ttttttctag tacctcattg atagatggtt ttaacataat tataattcct ccttaaattt
    17221 ctctattata atatttttat atctgctaac tttattcttt tcagatgata ttataccttc
    17281 tatttcttta gcagatgttt ttacatcttc attaaatata aaataatcat aatccttaat
    17341 cattttaatt tcttcatatg cacagctaaa tctcttctct atttcagctt ctgtttctgt
    17401 acctcttcca acaattctat tttttaattc ttcaagtgat ggtggtagta caaatataaa
    17461 tactccctct gggcaaactt cttttatttg ttttgctcct tgcatttcaa tctccagtaa
    17521 tacatcttgt cctttttcta aacattccat tattgccgct tttggagttc cataaaaatt
    17581 atcataaatt tgagcatact ccaaaaactc acctttttct atcatctctt caaacttttc
    17641 cttacttatg aagaaataat ttactccatc aacttctcca gttctgggtt ttcttgtagt
    17701 agctgataca gaaagtttta ttgtatcatt ttctttaagt agttctttgc aaatagtacc
    17761 ttttcctgca cctgatggtc ctgatacaac tagcaataat ccttttttca caaacattta
    17821 agcattcccc ttctaatatt gtattcttac tctatatttt gtatttgttc tcttattttt
    17881 tccaattcgc tttttacttc tactactaaa ttagttatat ttatatcaga tgacttcgaa
    17941 cctatagtgt tagtttctct attcatctct tgtattaaga aatctatttt tctacctata
    18001 gagtcgtctt taaatatagt atttttaagt tgccctatat gacttttaaa tctaactatt
    18061 tcttctgtta tactgctttt atctgcatat attgcaactt cttgtgctaa tctattctca
    18121 tctattatac ttgggtcatc cagtaattca cttattctca aattcaattt ttctctataa
    18181 tcttctacaa cactacttga atatttttca atctcttcta tatgattttt taataaatca
    18241 catctcatgg caatatcttc agctaatttt ttaccttctt cacttctcat ttctcttaat
    18301 ttgattaatg catcttcaac tgcttgattc aacattgacc acaataaatc ttcatcttct
    18361 tccttttctt ctattttcac aatatcagga aactttgcaa cattcataac gctaatatca
    18421 tctataatat caaatttatt ttttatttct tttagtatgt ctatatactg agatgctaat
    18481 ccctcatcaa aatttaagtt tacatcttct ttgcctaata agtcaaactt tatgtataaa
    18541 tctactcttc ctctttttac ataatttttt actaaatttc taactttatc ctctaaaaat
    18601 gaaatttttc ttggaagacg tatgtttata tctgaatact tatgatttat cgttttacac
    18661 tctactaaaa agtaataatt atcatccttg tattctcctc ttccaaaacc agtcatactt
    18721 atagccatta attaaacctc caaaacacct tcacatattt ttactgctgg acctgtcata
    18781 taagagaaat ctcctacatc tattttaact gttccgcctt gagacgttac atttacacta
    18841 ctttctacca tattaagtaa attgcacact attgctgatg cagtcatacc agtaccacaa
    18901 cctaaagtat atccacaacc tctttcccaa gtataaacat gtatattata cttatcttct
    18961 acctttacaa aatttacatt cgttttttca ggaaatattt tattattttc aatctctttt
    19021 ccatatttac aaacatcatc tacatctaat tcatctacta taactactgt atgaggaacc
    19081 cccatcaaca tagaagtaac tttaagctcc ctatctaaaa cctttatact ttcttgcaca
    19141 aaacgttctt tatttgtaag tacaggaacg tccttagcta taaaacttcc ttttcccata
    19201 ttgactctta tagagtttat ttctctattg gtaagattta tttttatatc ttttatacca
    19261 tctaatgtat ctactgtaaa actttctttt ttaactatac aattatcata aacaaattta
    19321 caaaaacatc taaggccatt tccacacata gctgctcttg aaccatcaga attataatat
    19381 accatttcta catctgcaac ttttgaatct ttcacaacta acaatccatc cgctcctaca
    19441 gaaaatcttc tatggcatac tttttttgct aaagcagtat aatcagatgg atttatctgg
    19501 tcgaatcttc catctattgc tataaaatca tttcctattc catgtagttt ccaaaatttc
    19561 ataagtatcg ctccttcgta aattaacgct taaactatta taacataaaa aaattcccat
    19621 aaacttataa tttatgggaa aagttttgta atgacaacta taatttgagg atgtgaggga
    19681 gagttgccat tacaattagg gtaattatat tatgttttta agctagttct ttctatgttt
    19741 atatattaat atttctatat gaattttatg tatgttttat ttaaactgtc tatgaatttt
    19801 ttaattaaac atctggtaac tagctataat ccttctagta gatatctatt aacatacacc
    19861 tattaattct ctaagtgttt ttaaaacata gtctaaatct tccttagtaa tatctacatg
    19921 agttacaaat ctatattcac cattttccat tttattaatt tttatatttc tttcaaaaaa
    19981 ttcttttatt aaaacatcct cttttataat atcttcactc atcttaaaaa ataccatact
    20041 tatatctgga ttaacattgt ttacttcaat gccatcaatt ttatttaatt cacaagctaa
    20101 atattttgca ttttcatggt cttcattcaa tcttaaagtc attttttcaa tggcaattat
    20161 accagctgct gcaactatac ctacttgtct cattccccca cccataactt tttgctttct
    20221 tctagctttt gctataaatt ctttacttcc agcaagtata gaacccattg gtgcagctaa
    20281 accttttgac acacagaaca taacactatc acaacacttt gctatttctt tagcttctac
    20341 acccaaagtt aatgctgcgt taaataatct tgctccatct aaatgtactg gtaagttatg
    20401 tttctcagct atttttttaa tttctcttaa attatcaaga ctcacaactg ttccacctga
    20461 atatgcattt tctacacata taagccctgt ctgtggttca tgtatgtcat ctcttctaat
    20521 tgccttttca acaagttcag gatttagagc tccactttcg tcttcatcta tagttctaag
    20581 ctgaactcca gctattacag caggtgcccc aacctcatga gtcacaatgt gataaccttt
    20641 accaataatt atttcttgcc ctcttaggca atgagtaaat agagctaatt gattcccaaa
    20701 agttccactt ggaaggaaaa tagcatcttc ttttccaact ttagctgctg ctaattcttc
    20761 aagtttattt acagtagggt catctctaaa gattgaatct ccaacaggag cctttgccat
    20821 agcttctctc atttcgtcag taggcatagt aacagtatca cttcttaaat cgataaatct
    20881 cattttttca accttctttc atatttatat actatagaac cttaaaattg attttttctg
    20941 taaagttcat atactttcat agcttatact ctacacaata attatactat atttacttta
    21001 tatttaatct actttaaatt attaatttta aaaataaagt ttcctctcta ataaatataa
    21061 ataattatac aatttaaata ttaatctcta ttgtttaaat tccccctagt cacaaagcgt
    21121 atattatgtg tataattaat ttactgactc attttggtca aagaaagggg tgctataaat
    21181 ggcattagat ggattagtta tacattcaat agtcgatgaa ttatcatcaa aacttacagg
    21241 tggaaaaata gataagatac atcaacctga agatgatgag gtaattttta atataagaaa
    21301 taataaagaa aattttagat tggttttaag tgcaagtgcc tcaaatccta gagtttatct
    21361 tacaagtaat tatcaaaaag aaaaccctct aaaagcgcct atgttttgta tgttacttag
    21421 aaaatacata caaggtggaa atatagtaga aatttctcaa ataggttttg aaagaataat
    21481 aaaaataagt gttgaatctt tagatgaatt aaaagaaaag acagttaaaa atattatgat
    21541 tgaaattatg ggaagacata gcaacataat aataactcat ggtgaagaaa ataaaattat
    21601 agattctata aaaagagtcc cttttagcat aagtagagtt cgtcaagttt tacctggtca
    21661 tgattattca ttgccaccag aacaaaacaa actcaaccct cttgatgata tatctaagga
    21721 tttgtttatt aaaaacctag aggaattgga aggacctatc tttaagtcta tatattctaa
    21781 atttctaggt ataagcccta ttatagctaa agaaatttgc tacagagcag gtgtaaatca
    21841 aaatgctatc ataaaggata tatctgatga acaatttgat gctttacaca aagtattttg
    21901 taatttattc aatgatataa attctaacaa atactctcct tgcattatta tagataaaaa
    21961 ggtggataag gtagttgatt tcagttgtat aaaccttaca ttatttagcg atttatcata
    22021 tataaataag gatagtatgt ctcgtattct ggaagatttc tatagaacta aagatattaa
    22081 agatagaatt aatcaacgtt catctgattt aaagaagagt atatctgtta agcttgatag
    22141 actttataat aaacttaaaa aacaagaaga agaattatca gaatccgaaa atgcagacat
    22201 ttataagata aaaggagaat tgattacttc atatatttat atggtagaaa aaggtatgga
    22261 aagtattgaa gttgctaact tctatgatga aaattgtaat gatgtaacaa tagaattaaa
    22321 taaaaacttg acaccatctg aaaatgctca aaaatacttt aaaaagtata acaaaatgaa
    22381 acatgctaaa gtcgagatat ctcatcaaat ttccttaaat aaggaagaaa tagattactt
    22441 agaaaacata attttaagta ttgaaaactg tgaaaattta gctgaattac aagatataaa
    22501 agaagaactt gctaaagttg gatacatcaa gactcaaaag aaaaacagta aaaaggatac
    22561 tatcccatct acaaaacctc atgaatttgt gtcttctgat ggatttaaaa tattggttgg
    22621 taaaaacaat aagcaaaatg actatcttac actaagactt gctgataatg atgacttatg
    22681 gatgcataca aagaatattc ctggttctca tgtaataata aagtgtgctg gtaaggaagt
    22741 ccctgataat actgtatttg aaggagctat gctagcagca ttctttagta agtcaaaact
    22801 ttcttctcag gtgcctgtag actatactaa gagaaaaaat gtgaaaaaac ctagtggttc
    22861 taaaccaggt atggttattt atgaaactaa cagtactatt tatgttactc ctgaagaaga
    22921 aacagtagcc aagcttaagg ttaaatctga ataaattctg cactaaatac aaaataaatg
    22981 ttagacaact acacattatc attctaaata aaaatagtgg ccttcttata aagtacaata
    23041 ataaggccac tttctaatta gcttactttt aattttctaa caaaatactt ttctgtttta
    23101 ataactatac ttatattccc aacaaatatt ttcagtcttc ttatatagat gtaattaaaa
    23161 agttaacact aaaagctatt ttttaattta ttgatttgtt ttagtaattc attatattct
    23221 gattcataca attctttttt tagattatct ttttctatag ataacataga tataacagtt
    23281 gtaagtctat tttctaatac tagaagattt tcttttttga tttggttttc tctactagga
    23341 gtttttttat ttctgctaat tatatattcg tcataattac aatcaaactc ccttatttta
    23401 gcatctttta tctctataat atagttacaa atatgtgata caaatactct atcatgagac
    23461 actataagca tagttttatc agtatttatc agtgcctttt ccagtgcttc catagatttt
    23521 atatctaagt aatttgttgg ttcatcaaat actaaaaaat tattatcttc caatattatt
    23581 ttacatagtg ctatttttac cttttctcca ccactaagta cttttacttt tttgtagaca
    23641 tcatcacctt taaacccaaa taggcttaag tttatcctta taaaagattc atcaaatgaa
    23701 ctattaactt tagtattgta tagaacactc ttttcctcat ctaataaact ttgattttgg
    23761 tcaaaataac ctacttttac cttgttattt atttttatat tgtcattttt atcagccaat
    23821 atttctttta tcagagtact ttttccacac ccattatcac ctaacaatgc tatcttttta
    23881 cctcttttta tcttaaagga aacattatct aacaggagct tattttctaa ctttagagtc
    23941 atatctttta cctctactag atttttgcta attatctcca taccatcttg catcttaatg
    24001 ttcatttctt tagaaacttt aggtttattc tttacctcaa gtttatcaat tctgcttttt
    24061 atagccttca catttgagtc caactttttc ttattagtct gaccacccat tttgtgcaac
    24121 ctagcttctg aattacccat tctttttggt gtttttctta tgccatcact taaagctttt
    24181 ttatataaaa gagctttttc aagccttttt ttctctttaa catattcttg atactcaaaa
    24241 tcagctcttt ttacttcttc atctttttgc attaaataat cagtgtaatt gcctttgtaa
    24301 gcttttagtt ttccatcttc tagttctaat atagttgtac ataactcatc cagaaaacgt
    24361 ctatcatgag atattaataa taatgctccc tcatgccttt taaacatatc ttccaaaacc
    24421 ccaatgcttt ttttatctaa gtttgaagtt ggctcatcag ctattatgat atttttctta
    24481 ttactcagcg catctgctat ttttaactta accttttccc ctcctgataa gtgttcttca
    24541 aatttatctg gagcatttaa aagactcttc tccttactat gaccacatgt ttcaatatta
    24601 ttttcactct gacttatata agaatagctt tctgtaaggt aagtatatcc ttcatcaatt
    24661 tctatttctc ccaatattgc ttttaataat gttgtttttc cttgaccatt tgaaccaact
    24721 agacctattt tgtcattttc taatatttca agtttatcta tatccaaaat taatttatct
    24781 gcataatatt tctttaaatt ctctactttt actaacaaca taaaaaatcc ctccttaaca
    24841 cacttaaata taaggagaga ttctataaat aggcataaca aataaacgta tagactataa
    24901 tctactctcc ttaatttaag cattgcaaat aaagccacca aaataggctt ttcaaaaatt
    24961 acaatacttt aaattataaa aaatagatta tattctcatt gtcctagtta ccttgccctt
    25021 tctttcattt atttttaatc attatatcaa cattaattac aaatgtctat agaaaattaa
    25081 ctaattttag ttattcaata tacaacttat tctttaaatt tattcttctt caactttaaa
    25141 gtcctgatta tttaaaattc tgtttaatac aattccaaca attgcagcta aactaagacc
    25201 agttatcttt actgtacttg ttatagggat tccaacagaa atacctttat tagataaata
    25261 tgttgttcca agacctatta ataatataac tgctatgatt aatatattta ttttactttc
    25321 tacacaagaa ctgtccctta tagtttttaa tcctacataa gttatcatcg aaaatagcat
    25381 tatactaatt ccacccataa ctgaacctgg tatactttga agaaatccac caaacttgcc
    25441 tacacatgca agcaatatag caaatattgc tgtacgtcta agtattgaag ggtcatagtt
    25501 tttagttata gcaagtactg ctgtattttc tccataagtt gtatttgctg gtcctcctaa
    25561 acaccctgca actatagtcg caaatccatc cccaagtaat gttctattta atccgggatt
    25621 ttctataaag tttttaccta ctacagcacc attagtagtc atatctccta tatgttccat
    25681 aaatacagct aatacaactg gagatataat cacaattgct cctaaactga acttaggaac
    25741 tgtaaagtta ggtattgcaa ataaacttgc ttctgtaata gtagttatat ctactaatct
    25801 taatatcaaa cagattatat acccagaaaa aactgctatt aatattccta attgttttat
    25861 aaatcctcta ccaaacttat tggttaaaat tgctattgct aaagttataa atgctattac
    25921 aaaattggct gaagccatac taaaagctgt tggcaataaa tttaatccta taactactat
    25981 catagctcct gtaacttgtg caggaaaata taatttaatt ttatttacac caactatttt
    26041 tactataatc gacattataa tataaattaa tcctgctact attataccac cttgagcata
    26101 tgctaaatct ccaccatacg cctctttagc tgctagtata actggtataa acgcaaaact
    26161 tgaacctaaa aaggcaggta cttttccttc tgtacaaaaa tgaaatatca atgtaccaac
    26221 accagcacag aaaattgcaa ctgacggatt aaaacctgtc aaaatgggaa ctaatactgt
    26281 cgctccaaac atggctaata gatgttgtaa agacattatc gtttttttca taaaaacctc
    26341 caaaattatt atatataaat aaatcctctg tttaattaat atcttttaat taattatcat
    26401 tccctagatt ctatttttta acctctatat acttattata taatttatca ttattttaca
    26461 ttttacgaca tcaaaaactc caataaagga gaataaaaac tacattaaga ctcacatcca
    26521 taaaattata atatttattt tgtgcaatct atgtaagctt tatcctcctc cctgactatt
    26581 caatgctaag cttttatata atacttaaaa ataattattc tttgattgta actgaatctt
    26641 ctccatcaat ttctaataaa ctgacagata taatttcatg tctagaagta ggtacatttt
    26701 ttcctacata atctgctctt ataggcaact ctctatgccc tctgtccact agcactgcta
    26761 attgtattga tttaggtctt cctatatcta taatagcatc tagtgaagct ctcacagttc
    26821 ttccagtgta taaaacatca tcaactaaaa ctactgtctt atcatttaca ttaaaatcta
    26881 aataagttcc tttaactaca ggttcaacat gaattttttc caaatcatct ctatacaaag
    26941 ttatatcaat atctcctgta tctacttttg ttccttctat ttgttctatt ttcttagaaa
    27001 ttctatttgc aattggcaca cctctagtct ttattccaac taaaactaga ttttctacac
    27061 ctttatttct ttcaattatt tcatgactta ttctagtaat tgctcttgct attgcctttt
    27121 catccattaa ttgagctttt tctaccatat cataattatt gtcaaagata taatatatct
    27181 atttgacaaa tctactcctt tcttttaatt ttttataaat ttatttttat ttaaaatttc
    27241 aatttaaaca acaaaaaaac ttctcatcac aagacaagaa gctcttatat aaaaaatata
    27301 cagtgcaatt tagcaattgt cttacgatat ctctgtatcg tttaaagata attttttaat
    27361 ctatcaaatc aaattatata tttatatttc aaatttttca agctttttta aacaaaatac
    27421 tttaatataa gatattttat ttaaatttca ctaaatttat ttacttatat aaaaacaaaa
    27481 aatgatttat aagtattata caaatggtta ttttatcttt ttaataactt ccttaaaata
    27541 ctcaggtaat tctgaattaa attcgatata ttccttagta gtaggatgta taaatcccaa
    27601 cttcttagcg tgcaaagttt gtccttttaa tttaaattta gtttcttttg gaccatatac
    27661 ataatcacct aaaagtgggt gatttataga caaagcatga actcttattt gatgagttct
    27721 accagtttca agcctagctc tcatatgtgt aaacttatca aatctttcta caacttcaaa
    27781 gtgagtgatt gcattttttc catcttttac tacagccatt tttaatctat ccttaggatt
    27841 tctccctata ggtttatcaa cagttatttt atcttcttta accacaccat agcatataaa
    27901 ttcatactcc ctagttatag tatgttcctt taactgttct gctagaaaat tatgtgcata
    27961 attgttctta gctatcataa gaagaccaga agtatctttg tctattctat gaactatacc
    28021 aggtctaata actccattta ttgatgacaa cttatcttta caatgatata aaatcccgtt
    28081 tactaacgta ttttcatagt ttccaggagc aggatggaca accatatcct gaggtttatt
    28141 tattattaag acatcatcat cttcatatac aatatctatt ggaatatctt gaggtaaaac
    28201 ttctaatact ttaggttctg gtatctgtat aacaatatta tcttcctctt taaccagata
    28261 tctagctttt tctactttat tattaacctt tacttttcca tcttttataa ttttttgtat
    28321 ataacttctt gacatatcac ctaattgttc tgctagataa atatcaagtc taacttcttc
    28381 ttcctcttca agtactaaaa actcttttac ttcatccatt atatcaccta cttttacttt
    28441 caaaaaataa aacatatata cataaaaaca cagttcctac aactacaaat acatcagcta
    28501 tattgaatac atattcccat ataattctaa agtcgaagta atctactaca aaacctaatc
    28561 gtactctatc aattagattt cccaatgcac cagatataat taatataatt cccaaccttc
    28621 caaatatatg tactttcctt gtattaagat agtatagtcc aaatactgtt gcaagtaatg
    28681 caacaattat aaatatccat tgattatttt gtaataatcc aaatgctgca cctctatttt
    28741 cgacataagt taaatgaaat acattattta ttattggtat tgttgataca tccaccaaat
    28801 tattcaatac ccatatttta gacagttggt ctaaacctat gagtagaatt attattaata
    28861 tatatagcaa tgatttttcc ccctataaat tatttaaaaa ccaattttat ttgaagcaca
    28921 ctattattat tatacaaaaa aaatgctcta aagagcaact ttattaaaat tgtattttct
    28981 tagtaaagct agaacaaaat ttaaaatagt actccttttt ttatattgta gatttctaca
    29041 tatttaaaat catatcatta tgtataaaaa atttaaaaat accatactaa tcaatacttt
    29101 ttgtgtattt tatagattaa agattaatga tgtctgtact ttaaacttac aagcaccact
    29161 aatcttcaaa caaatatttt tacttttgca cttgcttatt tttatctaaa tgttgtataa
    29221 gttcagatac tgttacgaat ttatatcctc tctcttttaa tttaggaatt actacttcta
    29281 aagcttgtat agtttgtgat ttatcatttc ttttagtgtt ataatcatgt aaaagtatta
    29341 tattaccatt ttggaccttt gtaaggatag tatttataat tgcatctact ccagggttag
    29401 accaatctct agggtctaag tttgaccaaa gaattatatt catgccttta gaaactataa
    29461 tatcacagac acttttactc atggctctat atggaggtct aaatatcttt ggttctactc
    29521 ctattacact ttttataata ttttgcgtat ctgttatttc tttttcaaca ctactataac
    29581 tattttttgc aacatttata tgtgtaaatg tatgattccc tatttcatgc ccctcatcat
    29641 attgtctttt taataattct ttgttatatt ctactttttc tccaactaca aaaaaagttg
    29701 cttttacatc atttttcttt aatatatcaa gtacttgtgg tgtaaaatcc tcatctggac
    29761 catcatcaaa agtcaatgct aaaatttttt catcctcatt tccttttttt attattacat
    29821 cttcataatc gtcttttata ttgtcattta aactatatgc ttcttttgaa ctatcttgat
    29881 tatgtgtatt atgattatat gtatatacac ctactataga taataaacat atacatatac
    29941 cccatattat tttcttcatg tcattacctc ttttctaatt cattctagca atatatttta
    30001 taatgtcagt tttttaatct ctcaatattc catttttatt taaatcagtt aaatttgtta
    30061 ggtcttctga ataaaagtta gaatcttcta catcagggtt ttgcattgca tcacctaata
    30121 aattattttc tttctttgca caatcattac ataaacttgt atctggcaat atatccaatc
    30181 tttctaaatc tatttgatta tgacataaat cacaaatccc atatgttcca ttctctattt
    30241 tagataatgc taagtctatt tgatacaatc ttccctcttc atgatttatt agactgtttt
    30301 gcatattttg catatataaa tctgttccca tgtctcctat atgattatca taactagata
    30361 attctcctga tgaatacttt tcacttgtat gtttagttgt atctccaaat aaagtattat
    30421 ctttcatatc ctctactaaa ttagttaaat tttctctttc tttcaataat ttatctttat
    30481 attttttagt atccattttt aacacctcta attttaatat ttacatcata aaaatagtat
    30541 gtggaaaagt aaattaaaca tacaataaaa ataagatttg attcgaaaag tagaatgtct
    30601 caaaatgaaa tttttagttc atttaaccct cttttttata taaaatcaaa tatattttta
    30661 tcctttcagc aataaaaaag agacttatct ctattttgag atagcctctt ttttgaacat
    30721 aatttaaata caatttttta atttttatag ttattatttt actaacttgc caatttatga
    30781 ttattttgtt ccttaatatg ctttgctttt ttaaagaata aaagaccaag aattattgct
    30841 acaataacac ctattccatt agatatatta actggtagtc taaatccttc ttttgcttgc
    30901 attatataac taaatgttac tacagtcata aaaagtgctg gtaatgtagt tatataatgg
    30961 tttttatttg ctttgactaa atatgcagaa ccagtccaca acattatcat tgcaagtgtt
    31021 tgatttgccc aagagaaata tctccaaatt atattaaaat ctataaatgt aagagcaaca
    31081 ccaactgcga ataatggtaa agctattaaa aatctatttt tgactgcatc ttgattataa
    31141 ttcattgcat ctgctattgt cagcctagca cttctaaatg ctgtatctcc agaagttata
    31201 ggacatgcca caactccaag tatagctaaa gctcctccaa taggccccat taatccaaca
    31261 gatactgtat ttactactgt tgctgctcca ccatcattaa atatcacatt aagttgaggt
    31321 atgccatgga aatatgacat ggctgctgct gcccaaacta aagctattat accttctgca
    31381 accattgacc cataaaatac tggtcttgcc tcactctctt tctcaacaca tctcgccata
    31441 agtggtgatt gagttgcatg aaaacctgat attgctccac atgctattgt tataaataag
    31501 tatgggaata aaggtgttcc atttggattc atattttgta aatttatatt tggtatgtta
    31561 taccccttta ttattaatcc tccagctaca cctactgcca taattaataa tgctgctcca
    31621 aataaaggat atattttacc aatcaattta tctattggta aaacagtagc cataatataa
    31681 taaagtatta tcactccaac ccatatttgt gcgtttacac ctgtcaatgt ctttaataat
    31741 cctgctggac ctgttacaaa tacaactcca accaatacta aaagaataac actaaatatt
    31801 ctcattgcta tttttgcacc atttcctaga taaaggccta ccaactctga tgcagatgta
    31861 cctttgtttt tcattgatag atatcctgaa gcaaaatcgt gtactccacc agcaaagatt
    31921 gttccaaaca caatccataa aaatgctgat ggtccaaata atgccccttg tatagctcca
    31981 aatatcggac ctaaccctgc tatatttaga aactgaatta aaaagattct cttccatttc
    32041 attggcacat aatctactcc atccctacaa gctatagctg gagtttgatt tttgtcattg
    32101 attccaaaaa ccttttctac aaaagtacca tagacaaaat aacctactat aagtactact
    32161 attgaaccta aaaagcttac cataaattca tccccttagt ttttatataa ttgctttata
    32221 aatttttatg agtttaagaa aactatttcc agttcaaata gtaatacttt tttatgctaa
    32281 tataaatatt aattacatta agtgcatatt taagtacatg aaatgacttt atatatgcat
    32341 taacagccta aattctttta ctttgcttct acttacagtt acttcatagt cactattttt
    32401 taattttaat atgtatgtat tattaaacca tggtataatt tcttttactt tggttaagtt
    32461 tacgatataa gacctatgtg ttctaaaaaa tgttttatca tttataagat tttcgacttc
    32521 tgatatgcct tcttttaata taaacttttc tttctcagta tatataaatg tctgcctttc
    32581 attagcttca cagtaatata tatcatcaat atctattaca actaatttat cacctttcca
    32641 aaggctaatt ttatgtgtta tttcttctat ctcctgattt actgcttcct tcttatattt
    32701 gtataaatta ctatttacat tagatagctc aatatcactc atctcacttt tttctaattt
    32761 attcagcata gaaataattc gctcttcaga atatggtttc aatatataat caaatacatc
    32821 caaagaaaaa gcatctactg cataactttc atatgctgtt ataaaaacaa ttttaggtct
    32881 tgatttaaac tgatttaatg tttttgcaag tagcattcca tcaagatgag gtatatttat
    32941 atctaaaaaa attacatcta ttttattttc ctgaataaag tctaacacct ctataccatt
    33001 ggtaaattca cttactacct cgattccact tttattttca ataaaatatc tcaattcctt
    33061 tcttgctgga aattcatctt ccactattat agctctcata cttctactcc tttacataaa
    33121 atactatcaa agtcccatta tctattcttc ttatattaag ccctcttcca tacatcagtt
    33181 taagccttaa atgtacatta taaagcccaa tcttattttc tggcatatta tcttgatata
    33241 cattatttat aatttcctca cttataccta taccattatc ctctattgat attctcacag
    33301 tattttctaa tgaatacggt aactttttta tggatatttt cactacacca gctctaccac
    33361 tttctaaaat accatgtatt attgcatttt ccactaatgg ttgtataatc aaacttggaa
    33421 tctttatatt tacatcttct atatcataag atacagttag taattctcca aacctagctt
    33481 tttctatctc tacaaaagat ttaacttgtt caatctcttt atttatatca attaagttat
    33541 cactatattc aagattatat cttagataag ttgataaatt tattattaac tctctagcct
    33601 tatctggact aaatcttata aatgaagcta ttgtgtttag ggaattaaac aaaaaatgcg
    33661 gattaatttg tctttgaagt gcttttattt ctgctttatt tctcgcttct atatcttttt
    33721 tatctttctc aatcccctct actatagata taacaaatcc tattccaatc tgcccaacta
    33781 ttaaaggtaa atatattcca ccgattattt gaattgctaa agaatgtgga tatgatatta
    33841 agtatataag taatatagta aaactttcag aaatcatgcc taccaatatt ccatacatta
    33901 ctctatgttg ttttggtatc ctcttatata gaaatcctga taatacacct gctagtatac
    33961 ttgataaaag acatggaaca gatgttatcc ccccaatatc cataaaatat ctatgaatcc
    34021 ctgaaaaaac tcctgctgta atacctacca ttggaccaaa taaaatgcca cctgatacta
    34081 tagatattat tctagtgttt actattgacc ccatataatt tataccacta taagttccaa
    34141 atatggctaa aaaagtaaaa actaatgcta tacagaccaa gtcattaaaa ctatattctt
    34201 ccttttgaaa tatctgctta aatattttta actttgttat tagaagaaac aatactacaa
    34261 taaggcttgt cttttctatt aatgcgatta aaattgatgt tttcataatc cccctttaaa
    34321 aaataaattt ttacacactc taaattttat tatggttgtt ataaattaca tatttgaagt
    34381 aatcctatca caaaactttc cacatttaat cttttaatta aataagaaat agttgtactt
    34441 ctattaacaa ctaatactag ctagcataag cttgtaataa cttgtagtta ttattaactt
    34501 tacaattcta gtatttaatt ttatacttta gaaatctctt ttcttagaaa taatactttc
    34561 gaatatcata taatacaata ttatgtagat tcttactcat acaacatatt tatttaaaaa
    34621 ttttatacta ttctttgttc aaatatctca tttgatttaa ttgtgttaaa cacacattat
    34681 catctctctg ttatatattt attatatagc cgtatatcga taaagtaaat aaatcccata
    34741 tttaaaataa tatgtt
//
LOCUS       DAEMHZ010000029        34738 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000029 DAEMHZ010000000
VERSION     DAEMHZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.029"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..823
                     /locus_tag="KPK72_001293"
     CDS             <1..823
                     /locus_tag="KPK72_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2488526.1"
                     /translation="YTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKL
                     KYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYW
                     FNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGL
                     HKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKD
                     VTNSAIVTMIEFTYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            1739..2761
                     /locus_tag="KPK72_001294"
     CDS             1739..2761
                     /locus_tag="KPK72_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2488527.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3057..3977
                     /gene="pyrB"
                     /locus_tag="KPK72_001295"
     CDS             3057..3977
                     /gene="pyrB"
                     /locus_tag="KPK72_001295"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG2488528.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4016..4708
                     /locus_tag="KPK72_001296"
     CDS             4016..4708
                     /locus_tag="KPK72_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG2488529.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            4701..5603
                     /locus_tag="KPK72_001297"
     CDS             4701..5603
                     /locus_tag="KPK72_001297"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2488530.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRTVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            5688..6272
                     /locus_tag="KPK72_001298"
     CDS             5688..6272
                     /locus_tag="KPK72_001298"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG2488531.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6487..7413
                     /locus_tag="KPK72_001299"
     CDS             6487..7413
                     /locus_tag="KPK72_001299"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG2488532.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEVDFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMMKVIESRF"
     gene            complement(7465..8355)
                     /locus_tag="KPK72_001300"
     CDS             complement(7465..8355)
                     /locus_tag="KPK72_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2488533.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8543..9574
                     /locus_tag="KPK72_001301"
     CDS             8543..9574
                     /locus_tag="KPK72_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG2488534.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            9868..10743
                     /locus_tag="KPK72_001302"
     CDS             9868..10743
                     /locus_tag="KPK72_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG2488535.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            10761..12251
                     /gene="cls"
                     /locus_tag="KPK72_001303"
     CDS             10761..12251
                     /gene="cls"
                     /locus_tag="KPK72_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2488536.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12444..12728
                     /locus_tag="KPK72_001304"
     CDS             12444..12728
                     /locus_tag="KPK72_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG2488537.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            12761..14389
                     /gene="groL"
                     /locus_tag="KPK72_001305"
     CDS             12761..14389
                     /gene="groL"
                     /locus_tag="KPK72_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG2488538.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVIKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            14651..15391
                     /gene="larB"
                     /locus_tag="KPK72_001306"
     CDS             14651..15391
                     /gene="larB"
                     /locus_tag="KPK72_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG2488539.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15475..16209
                     /gene="larC"
                     /locus_tag="KPK72_001307"
     CDS             15475..16209
                     /gene="larC"
                     /locus_tag="KPK72_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG2488540.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            16239..16658
                     /locus_tag="KPK72_001308"
     CDS             16239..16658
                     /locus_tag="KPK72_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG2488541.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKLSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      16718..16819
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17040..18575
                     /gene="guaA"
                     /locus_tag="KPK72_001309"
     CDS             17040..18575
                     /gene="guaA"
                     /locus_tag="KPK72_001309"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG2488542.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            19114..20544
                     /locus_tag="KPK72_001310"
     CDS             19114..20544
                     /locus_tag="KPK72_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2488543.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(20692..21042)
                     /locus_tag="KPK72_001311"
                     /pseudo
     CDS             complement(20692..21042)
                     /locus_tag="KPK72_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            21037..21558
                     /locus_tag="KPK72_001312"
     CDS             21037..21558
                     /locus_tag="KPK72_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2488544.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAFAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(21687..21923)
                     /locus_tag="KPK72_001313"
     CDS             complement(21687..21923)
                     /locus_tag="KPK72_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488545.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEVYDWFVENQ"
     gene            complement(22233..25661)
                     /gene="uvrA"
                     /locus_tag="KPK72_001314"
     CDS             complement(22233..25661)
                     /gene="uvrA"
                     /locus_tag="KPK72_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2488546.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDIGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSNCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            25980..28214
                     /locus_tag="KPK72_001315"
     CDS             25980..28214
                     /locus_tag="KPK72_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2488547.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28470..30500
                     /locus_tag="KPK72_001316"
     CDS             28470..30500
                     /locus_tag="KPK72_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2488548.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFRVSRSSIKNDLNVLSSELERNQIIITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDDQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30497..30949
                     /locus_tag="KPK72_001317"
     CDS             30497..30949
                     /locus_tag="KPK72_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2488549.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            30951..32006
                     /locus_tag="KPK72_001318"
     CDS             30951..32006
                     /locus_tag="KPK72_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG2488550.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32073..32381
                     /locus_tag="KPK72_001319"
     CDS             32073..32381
                     /locus_tag="KPK72_001319"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG2488551.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32485..33750
                     /locus_tag="KPK72_001320"
     CDS             32485..33750
                     /locus_tag="KPK72_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG2488552.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVRFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            33838..34551
                     /locus_tag="KPK72_001321"
     CDS             33838..34551
                     /locus_tag="KPK72_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2488553.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSKKC"
BASE COUNT        13405 a         3751 c         6093 g        11489 t
ORIGIN      
        1 ttacactata tttaaatata taaaacttaa aaatttaata aatgacttat cttatgatgt
       61 tgatgatgga gagattgtaa atctttataa aaatatatta gaagaattta atataacaaa
      121 agatataaaa ctcaaatact cttatgaagt tgaaacacca gcatttttta attcatgtgt
      181 gcttttgcca ccacatgagt ataaattgaa agagttagac tggattttta gacatgagct
      241 tatgcatttt aagagtaggg acctttattt aaaatatgta atcctgtttt taaaaacagt
      301 atattggttt aatccattta tatatataat ggataaacat atagatttag attgtgaact
      361 ttattgtgat gaaagagttt taaaaaatcg aaattcagag gaaaaaaagg attatgcttt
      421 aacaattcta aatgcaatga gaaaaggttc aaatacttca aataaattta tagctggact
      481 ccataagcaa tctgatatta aaaagagagt tatgaatatg tttaatgaaa agtacaagaa
      541 tggtatatta atggcattaa ctttatgttt attatcttct attacttttt tgaaggttaa
      601 ttcaacgagt attgttaatc ttaatatatt gccaaaagga atggaagaaa aagatgttac
      661 taatagtgca attgttacaa tgatagaatt tacatatgca gaagctccta aaagtatgag
      721 agaagaacat aaaataaatt gtgaattctt aggtttaata cctaaagact ctgataaaat
      781 aggtgttggt atagaatatt atgacagtct attgaatgat taaataaata ttattttaac
      841 agatattaat tataaagttt tgaatagtaa tttgttgaga aataaatata tccttataaa
      901 aatagatact tatataccaa gaataaaaaa tagttaagat agtacacaag gatatactta
      961 taaagaactt aaataagtga aataaattat taattataag gtttataatt ttaaaaaatt
     1021 agataaattc aggtgtaaaa tactataaaa cacaattcgg aaaagtgtat gttgaacttc
     1081 atttaaaatg gaatttcaaa gttgctatag taagtagttt aagcattctt cttatattaa
     1141 tctttgagaa taatgtattt aaaaaagtta gtaaaaaatt taactattgt atattaatga
     1201 tagttgctat tgatatatta tctaaaaaaa ttaagtatga aaagattact gctaggacaa
     1261 tcttttcata cttaattttt aatatttata ttctccaaaa ttttcatatt tttaacttag
     1321 aatttgagaa tcactattag gaaataggct ttgatttatg tagggttaaa aatttcacag
     1381 aatatctaac tttatactaa atccatcaaa aaatatacgc catataaaag cgcataaaaa
     1441 aacacataaa acaatattta tgcgcacttt aacctcgaat ggtacaaaat tctttacttt
     1501 tacagaccca tattatataa ttaagtaaat aatgtatatt taggtgacat attggataag
     1561 ctaatttatt tattttatta atataataat actataaaag ttatataaag tggtttacaa
     1621 aaaagttaca gatagagctt ttattaatgt attgtgtatt agtattttat ccttaggtac
     1681 acttcaccta caaaatagaa tcataagata attatgggaa aattttagga ggtttatcgt
     1741 gaagaaaaag atattaatac cagtatttgc atcagttatg gcattatcag taagttcaat
     1801 tgtaaatgca gatgaagtaa atgattcatc tcaaaataag gatgataaaa caaatactga
     1861 attaaattta ggtgaatata aagaagtaaa atataaagtt gcaaaaataa aagatggagt
     1921 agctataaag ataagagaag aaggtcaagt tcaaaacata gcttattctg gagatgaatt
     1981 tacggtttta ggaactcaag gagaatgggt taaggttaaa gttgaagatg gcgaaggttg
     2041 gttagctact agatatgtag atatatctga aggtgtagga tatactaacg ctgataaagt
     2101 aaacttaaga aaagataaga gtgaaagttc agaagtaatt gaagagctgg aaaaaggaag
     2161 ttctttattg gttttggaag ataatggaga ttggttaaaa gttaaagatg gggaaacaga
     2221 aggatatgta aaatcatctt atatatctga taaagctcct gtaatagaag agccacaagt
     2281 aaacccaagt attgaccaaa atgtagattc tacaaataat aatcaacaaa acaatgcaaa
     2341 tactaatcaa caaaataata attcaaatgt gccaacagct aactctaatg cagttcaagc
     2401 tgtacttaac ttggcatatt ctaagcaagg ttgtccatat gtttggggag cagaaggacc
     2461 aaacactttt gactgttctg gatttacaca atatgtgtat agaaatgcag taggaaagaa
     2521 tataccaaga acatctaaag cacaatctaa atatggacaa actgtaagta aagcaaattt
     2581 acaaccagga gacttagtat tcttcacaac taatggttct ggttctgtta gtcatgttgg
     2641 aatatatgta ggtgggggta atatgataca ttcaccatca acaggaaaaa cagttagtgt
     2701 aactagtata aattcatcat actatacagc aagatttgtt acagcaaaaa gaatattata
     2761 atcggtttgt aatatgacaa attgataaat acataaatat atataaacgc tctatattat
     2821 atttaattta atcatggaaa caaaaaatga ttatatttta aatgtactat agagcgtttt
     2881 tgtatcactt taaatctaat caagattcaa gttaatatga ctgttaaata ggagcctttt
     2941 catatgatga ttatttgcgc aatcccttga caggataggg caaaggtggt aagctactaa
     3001 cagtacaaaa atacttttaa ttttagttca gagaggctaa aaaaggagga cgaaagatgt
     3061 taaaatcaag aaatttaatc caaccagaag acttttcaat agaagaaata gatgaaatat
     3121 tagaattagc tcaaaaaata attgacaatc cgtcaaaata ttctcgtata tgtgaaggaa
     3181 aactattagc tacattattt tatgaaccat caacaagaac aagattaagc tttgaatctg
     3241 ctatgaacag attaggagga cgtgtagtag gcttttcaga gccaaattca tcctctgcat
     3301 caaaaggtga aactctagga gatacaatga ggatagtatc tggttatgtt gatataatag
     3361 ctatgagaca tcctcaatca ggtgcagctt cagaagcagc tagatatacg gaagtgccat
     3421 ttataaatgc aggagacggc aagaaccagc atccaactca aacacttaca gacttactta
     3481 ctataaaatc attaaaagga actttagaaa gccatacaat aggattatgt ggtgatttaa
     3541 aatatggaag aactgtgcat tctttggtta aggctatggc aagatataaa aatacaaagt
     3601 ttgtatttat agcgcctgaa gagttaaaaa tgccagatta tataaaagaa gctataaagg
     3661 gacatgcata ttatgaaact aataatttgg atgatgttat aggaagcttg gatgttttat
     3721 atatgactag agttcaacaa gaaagatttg aagataaatc agagtatgaa agacttaaaa
     3781 actattatat tttaaataaa gctaaattag aaaaggcatc taaagatatg ttagtaatgc
     3841 atccattacc aagagtaaat gaaatagaca tagatgtaga tagtgatgat agagctgtat
     3901 attttaaaca agctaaatat ggaatgtatg ttagaatggc tttaataata aaacttttag
     3961 gtataaatga agactaatag ttatcatata atagtaaaac aaaggagatt ttgggatgta
     4021 taaaattctg gaaaatatat atataggcga agatatgtat agaatgaaag taaaaggtaa
     4081 ttttgaaggt aaaatgggac aattctatat gttaagagca tgggatactt atccagtact
     4141 ttcaagacct ataagtatac atgatataga tgaagaaggg ataacttttc tttataaagt
     4201 agtaggagaa ggaactcaaa ttttgagtaa cctaaaggta aatgataata taaaattaga
     4261 aggcccttat ggtaatggat atgctaaggt tgatggaaag gtagctctag taggaggagg
     4321 aatcggtgta gctccactat atttggttgc taaaaatata aaaaattgtg atgcatatct
     4381 tgggtttaga gaagatgtta tattagaaga tgaatataaa caagtatgta ataaagtcta
     4441 tacaacagta ggaaatactt ttgtaacaga tataattgat gttgagaaat atgattatat
     4501 attaacatgt ggtccaactc caatgatgga aaaattagtt aagatggtag aaggtactaa
     4561 aacgagaatt atggtatctc ttgaaaacca tatggcttgt ggagtaggtg cctgtttagt
     4621 atgtacgtgt aaaactaatg gaggaaataa aaagacttgt aaagatggtc cagtgttctg
     4681 gggagaggac gtgattttta atgggtaatt taagtgtgaa atttggaagt gtagaattta
     4741 aaaatccagt aataatggcg tctggaactt ttggatttgg aaaagaatat aatgagattt
     4801 atgatataca aaaacttggt ggtataagta gcaagggtct tactttaaat aaaaaacctg
     4861 gtaacaatgg tatgagagtt catgagacac catccggtat gatgaatagt gttgggcttg
     4921 aaaaccctgg agttcaagga tttatagact atgaattacc tttttttagt aaactagact
     4981 tagtcagaat agctaatgtt ggaggtggga cattagaaga ctacttatta ggggttcaaa
     5041 tgttaaatga caagcctata gacattattg aattaaatat atcttgccca aatgtcaaag
     5101 ctggtggaat ggcttttggt ataaaaaatg aagtggcaag agaagttgtt cgtgaggtaa
     5161 gaaacataac gaaactgcct ttagtaataa aattatcacc caatgcagaa gatattgtag
     5221 gaatggcaaa agtttgtgaa gaagaaggtg ctgatggagt ttcattagtt aatactttta
     5281 aagcaatggc aattgatata aaaaatagaa gaacagtatt tgaaaatgta tatgctgggt
     5341 tatctggacc agctataaaa cctatagctt tgagaatggt acatgaagtg tgtaaaaatg
     5401 taaatatacc tgtaatggga atgggcggaa taactaaagc cacagatgct atagagttta
     5461 taatggcagg agctacttgt atccaagttg gaactgcaaa ttttatgaat cctagaatag
     5521 gtattgaaat aatagatgga attaatgaat ttatggatag agaaggaata aaaagtttag
     5581 acgaaataag aggaattata taaggtagaa taaatagtat tttaaagata aaaaatatag
     5641 attaaataaa caataatttt ataacattaa catggaggaa aaataatatg agtaaagtag
     5701 atgtagtgga tatattaaag aaaagtgatg cattgttgga gggacatttt ttattatctt
     5761 caggaaaaca cagtaataga tacgtacaat gtgcaaaagt attaagattt ccagaatatg
     5821 ctgctgaggt attaagtaca gttgttgaac aaataaaaga cttagatata gacttagtag
     5881 taggaccagc tatgggtgga gtaatagttt cttatgagtt aggaagacaa ttaggaaaag
     5941 aagctgtatt tactgagaga aaagacaata caatggagtt aagaagagga tttgaagtta
     6001 aaaaaggagc aaagataata attgctgaag acgttgtaac tactggtaaa tcaactatgg
     6061 agacaaaaag agtattagaa gccttaggtg gagaagttgt aggtgttgca tgtatagcag
     6121 atagaactaa tcatgatata ggtatgccta tatatagtgc tataaaactt gatattcaag
     6181 tttatgaatc tgatgagtgt cctttatgta aggaaggaaa attaccagtt gttaagcctg
     6241 gaagtagaga gttcaaggaa ttagggatgt aataaaaact taattattta tagtgttact
     6301 taaaaaatgt aaatttttta gagtaaaaat ttgtaatata acatataact taaaaataaa
     6361 aagcagttag ccttataaat gaaggttagc tgtttttttt gttaaaaata tatttacaaa
     6421 ataagagata gtagcagtac aatataagta taaggtaaaa gtattatata ttagggaggg
     6481 gtaagtatga aaaatcaatt aaatgaaaat cataaaaagg ttgtattttt tggagtaggt
     6541 gctgtaggag ctacttttgc agaacaattt tttaactcta aatatgattt taaaattctt
     6601 tgtgataatg aaagaaagaa aagatattta gaagaaggat ttataataaa tggaaaaagg
     6661 tatgattttg attatgtaac taaagatgag tataaacaag aggttgattt tataattata
     6721 ggtctaaaat ataataactt aaaagaaaat ataaaagaat tagatggatt agttggaaaa
     6781 aatacagtta taatgtctct gctaaatgga gttgatagcg aagagataat aggagaaaga
     6841 tttggaattg aaaaaatggt atattcatat gttaccaata tagatgcaaa gaaaatcaat
     6901 aataatatta tacacactac taatgggata attgtatttg gtaacaaaga taatagtgaa
     6961 gatagaaaaa ctaatataat aaccgaagtc tttgatgatg taaatataga atatacttta
     7021 tcaaaagata ttcaacgaga tatgtggtgg aagtacatgg ttaatattgg tgtaaatcaa
     7081 acttcagcta tacttggtgc accttatgga gtttttcaga gttctgagca tttaagagaa
     7141 ttagcaaaat ctgcaatgag ggaagttgtt gctatagcac aagcaaaaga catatctctt
     7201 acagaagatg atgtggaaca ttcattacat agaatactag aacattcaaa agaaggaaga
     7261 acatcaatgc ttcaagatgt ggaagctcat agacttacag aagtagatat gttttctaag
     7321 aatatctgca aacttggaaa aaaatataac atacctactc ctataaatca gactttcttt
     7381 tatatgatga aagtaattga aagtagattt taaagggtct ttgtaagaaa tattgataat
     7441 tattgatttt aaattttatt aatgttatat actatttaag ttactaaata tttttaactc
     7501 ttcaatgaaa atagttgctg cttttgataa agttatatct tttggaaata tatatgagaa
     7561 atgtctgttg tagctgcttc caagctctat gacagacagc tcattgttta gaacaggtaa
     7621 gcttgtcact agattagaga ctattgtaat tcctaaatta tttctaacag attctttgac
     7681 agcataatta cttccaaaaa ccatcatact ttttggaata atttctttaa cacttaaaaa
     7741 catatctaaa taatctctgg ttccagaacc atcctccctg actatccatt tttggttttg
     7801 aagtttatca aaactaaaat cttttaataa atgactcttg tatggaagtg ctaaaaccat
     7861 cttatcttca aaaaaatatt cttgaataaa tgaaggagaa gagcaagtgc cttcaataag
     7921 accaatgtct aaaatataat ccttaacatg agaagatacg attgatgtat tttttataaa
     7981 aacttctaca tctatgtcag gatacttttt agaaaataat gctagaaaat ttggtaaaat
     8041 atactctcca atagttaaac ttgcaccaat ctttaagtga ccagttatag catctgaaac
     8101 ttggcttaca tcatgataag taatatttaa aagatttaat atttctttag ctcttttgta
     8161 caagatttgt ccactttctg ttataaaaat ggttttctgt ttaatagacc tgtttataag
     8221 cacaacttta aaataatttt ctagattttt tatgtgttta cttacacttg gttgtgatag
     8281 atttaaatat tccccagctt tagtaaagtt tttgtattca acaacagcta caaatgtttt
     8341 taattcttca aacaattttt tgtctccttt acctaatgaa attttacatt ttttatatac
     8401 tttctacaga taattatact atatataata accattaaaa acattaatca ttacgatatt
     8461 atatatttat tttacttatg gttattacaa ttatatacta ttgctatgaa gaaaactata
     8521 ttaattataa ggtaggtata ttatgttaaa gaaaaaaagc agtttgacat taaaaactat
     8581 aagggaaata ttaccaggac tgtttgtatc agtattagta ggttatatta gtatatttat
     8641 ttctatttta attcctaagg taggggcagc atcaatttct atatttttag gtatgtttgt
     8701 aggaaaccta tttttaaatc aaaaggtatt tcaaaaaggt tataaatttt cagagacaga
     8761 tttgttatca tattcaatag tactattagg agcaacactc agtgtatcta ccttaataga
     8821 cttaaaagtt tcaggaattt tatttataat attgcaaatg acaataacaa taatagctgc
     8881 actttatata ggtaaaaaac taggatttga agaaaacttt agatttctaa tggcaagtgg
     8941 aaatgcagta tgtggttctt ctgcaatagg agcaacagtt ccagtggtta atgcgacaga
     9001 taaagaaaag ggaattgcag taactattgt aaatgttact ggtatatttc ttatgttttt
     9061 acttccaata atatctcaat ttttatatag ccatgaactt gtaaaaactt cagcaatgat
     9121 aggtggaact ttgcaatcaa ttggtcaggt tgtagcaagt ggagcaattg ttggagaaaa
     9181 tgtaaaagat ttagctacta tttttaagat agtaagagta atatttctag ttgttgttgt
     9241 attagtattt ggacatatga aaaatcaatc taatagggaa attcttgaag aagaaaagga
     9301 tgagataaaa aagaaaaaag tgactatacc atggtatgta attggattct ttataacttg
     9361 tgcacttttt tcaatgaaca ttataccaca agaagtatct gtgttgtgca aagaaataag
     9421 taacaaacta gaaataatag ctttagcagc aattggatta aaggttaatg taaaagattt
     9481 agtgaaacaa gggaaagaag tttctttata tggtttattt gtaggtatag tacaagttgt
     9541 atcagctgta gttttaataa aaatatttat atagcataaa aatagtattt gtaatattaa
     9601 tttgattaac taaaataata tgatactaat aatttataat ataaataaaa aatgaatttc
     9661 gataagttta agtgggtata aacttataga aattcatttt ttatttaaag taaaaattta
     9721 aagtaaaata ttattttaca taataataac cttataaatt ttgaaattac ttatctttgt
     9781 gttaaaataa gtaaaatgca catattagag ctaagttttt aagtaaataa acaaaatgaa
     9841 aatataaata ttaaggagat ttggtatatg aatgtttatg aaaaaggtaa tggagttata
     9901 ctagaaggag taacagattt tgacccagta catatatttg agtgtggtca atgttttaga
     9961 tggcacaaac aagaagatgg ctcatacact ggagttgcaa aggggagaat attaaatgtc
    10021 aaaaaagaga atgataaaat atatttaaat aatactaatt taaaggagtt taacagcatt
    10081 tggtataatt actttgattt aggaactgat tatacagaaa taaagaataa gctaaaaaat
    10141 atggatgaat atttaaataa ggctactgaa tttggatggg gaataagaat attaagacaa
    10201 gatggatggg aaatgcttat atcatttata atttcctcta ataataggat tcctatgata
    10261 caaagagcga tagaaaattt atcaagaaag tttggtaagt atataggtga atatgaaggt
    10321 aatgaatact atgcattccc aacaccagaa gaactaaata aggcctcaca agaggaaata
    10381 agagcatgtc aaacaggttt tagagataag tacataaaaa gtactacaca ggcagttata
    10441 gagaataatg atgaggtatc tgaatataca aatttaagta cagaagattg tagaaaagag
    10501 cttttaaaat ttaatggagt aggaccaaaa gtatgtgatt gtattgcatt atttggtatg
    10561 caaaaatatg attcttttcc agttgatgtt tgggttaaga gagttatgca agaattttat
    10621 atagatgaag acatgagttt acctaaaatg agaacttatg gaatcgataa atttaaagaa
    10681 atgtctggat ttgctcaaca gtacttattt tactatgcaa gagaactcgg tataggaaaa
    10741 taaattaagg gggagaaatt atgggagtaa taggaacaat atttctgttc tatttaatta
    10801 tatcatatct agctggagcc ataatttctg tgattatatt gcttgaaaat agagaccctg
    10861 caaaaacaat gtcttggcta ttgatgttta ttatatttcc aggtgtaggt ttaatgatat
    10921 atgctatttc aggtagaaat attagaaaaa gaaaattatt taagactcaa aaactagcta
    10981 ataatataaa agaaaagaaa ctatttgata cattagaaaa gataacagaa atagttgaat
    11041 tagaaaaaga atctataaaa caaaataagc ttttaagaga tgaagaggat ggtagttata
    11101 gaaaaagggt aataaatatg ttgttaaaga ctggtatgtt tccctttact aaaaataata
    11161 aagtggatgt atttgtagat ggaaatgaaa agtttaaaag acttatagaa gatataagag
    11221 aagctaaaga ccatattcat ttagagtact ttatcatcaa agatagtgaa ataggtaggg
    11281 tgttaaaaga agaacttata aaaaaggcta aagaaggcat taagataaga attttatatg
    11341 atgatgtagg atgctggaga ttttggttta atagaaaatt tttccgagaa atgagggaag
    11401 taggtataga aattgcagca tttttaccta caaaatttcc tataatagga gggaaattaa
    11461 actatagaaa tcacagaaaa attgttgtta ttgatggaat aattggatat acaggtggaa
    11521 taaatatagg agatgaatat ttaggcaaaa atgataagtt tggatattgg agagatactc
    11581 atattaggat taagggtatt tctgtataca tgcttcaaat gacatttctg attgattggt
    11641 attatacgac aaaagaagta ttagtgacta aaaattattt cccaagtgta gggaatgttg
    11701 gtgaaagtat gatacaagtt gttgcgagtg gtccagacag tgactgggaa gatattcatt
    11761 atgcttattt ttctgcaata tgtcaggcta gaaaaaatgt ttatatagaa actccatatt
    11821 ttattccaga tgaaagtcta ctaaaagcta taaagagtgc tgctcttagt ggagttgatg
    11881 tgagaataat atttcctaaa attgcagacc acaagatagt aaatatagca tcttattcat
    11941 attttgaaga aatattaaga gcaggtggta aagtttattt atataataaa ggatttatac
    12001 attctaaagt cgtaataata gatgataaaa ttgcttctgc aggtacagct aatatggatt
    12061 taagaagttt tatgcttaac tttgaggtaa atgcatttat ctatgatgaa gaagttatta
    12121 gggtaatgac agatgatttc tttgaggatt taagtcactg tgaagaactt aatttggagg
    12181 tatttaagaa tagaaatatc atacaaaaaa tcaaagaatc tgtagcaaga ttattttcac
    12241 caatattata aaaaattatt aaaaaaggta ttgaaaatta tgtgcgattg gtatattata
    12301 ataattgtta gcactctatt agatagagtg ataataaata aaaagtatat atataaatct
    12361 acatatataa gtagtattaa tttaaaaata actaaaaaca taggcgaact agtctatact
    12421 taatatttta ggaggcgtga ataatgaaaa taagaccatt agctgacaga gtagtaatta
    12481 aaaaagtaga agcagaagaa aaaactgcaa gtggaatagt tttaccagga gcagctaaag
    12541 agcaacctca aatagctgaa gttgtagaag ttggaccagg tggaatagtt gaagggaaag
    12601 aaataaaaat ggaattgaca gtaggagata aggttatatt ccaaaaatat tctggaacag
    12661 aagttaagat agaaggacaa gaatacacaa tactaagaca gagtgatgta ttagctgtta
    12721 ttgaataaat atagaataaa tttattagga ggggtttaaa atggctaaag aaattaaatt
    12781 ttcagaggaa acaagaagag ctttagaagc tggtgtaaat aaattagcag atacagtaaa
    12841 agtaacatta ggacctaaag gaagaaatgt tatattagat aaaaaatttg gttctccact
    12901 tataactaat gatggagtaa ctatagcaaa agagatagaa ctagaagata gatttgaaaa
    12961 tatgggtgct caattagtta aagaagtagc aactaaaact aatgatgtag ctggagatgg
    13021 tactacaact gctacagttt tagcacaagc tataataaga gaaggtttaa agaatgtaac
    13081 agcaggggct aacccaatac ttttaagaaa aggaatacaa aaagcagtaa cagtagcagt
    13141 agaagaatta aaaaatcaat caagaatagt agaaacacaa gaggctatat ctcaagttgc
    13201 ttctatatct gctggtgatg aagaagttgg aaaattaata gcagaagcta tggaaatagt
    13261 aggtaaagat ggagttataa ctgttgaaga gtctcaaact atgaatactg aattagatgc
    13321 tgttgaaggt atgcagtttg atagaggatt tgtttctgca tatatggtta cagatgtaga
    13381 taaaatggaa gcagttttaa atgacccata tatattaatt actgataaaa aaatatctaa
    13441 catacaagaa ttattaccag ttcttgaaca aatagttcaa caaggtaaaa agttattgat
    13501 aatagctgaa gatgtagaag gtgaagcatt atctacatta gtagtaaata aattaagagg
    13561 aacatttgat gtagtagcag ttaaagctcc aggctttgga gatagaagaa aagaaatgct
    13621 tcaagatata gcaatactta caggtgctca agtaatatca gaagaattag gttatgattt
    13681 aaaagaagct gatttatcta tgctaggtag agcttcatct gttaaggtaa ctaaagaaag
    13741 tactacaata gtagatggtt ctggagataa aaaagctata gaagatagag taactcaaat
    13801 taaacatcaa gtagagcaaa ctacttcaga ttttgataga gagaaattaa tggaaagatt
    13861 agctaaactt gctggaggag tagctgttat aaaagttgga gctgctacag aagtagagtt
    13921 aaaagaaaga aagttaagaa tagaagatgc tcttaatgca actagagcag ctgtagaaga
    13981 aggaatagta gctggtggag gaactgcttt tgttagtgtt ataccggcaa taggaacact
    14041 aattgagagt ttagaaggag aagttaaact aggtgctcaa atagttaaaa aagcgttaga
    14101 agagccatta agacaaatag ctataaatgc tggtcttgaa ggtgctgtaa ttgtgcaaaa
    14161 tgttgttaat tctgaagcag aaactggatt tgacgcattg aatgaaaaat atgtaaatat
    14221 gatagaagct ggtatagttg acccaactaa agttagtaga agtgctttac aaaatgctgc
    14281 atcaatagca agtactttct taactactga agctgctgtt gctgaccttc ctgaaaaaga
    14341 agatgcagga atgccaggaa tgggtggcgg aatgcctggt atgatgtaat ttaaaaaatt
    14401 taaaatataa attttaatat aaagaactat ttcgacttag attttaaact ctaagttgaa
    14461 atagttcttt tgtatatatc taatttttgt atatctataa attcaataca catatttaga
    14521 taatcagtat ctagtttgta ataatatttt agagtagtat tgttattttt taggttatga
    14581 ttttagatat atattaatta aggtataatt gttgatagag aataaatcaa atttaaaagg
    14641 atgcgataat atggacttaa gaaaattgct tgaagaagtt aaaaatgaaa atattgatat
    14701 agattttgcg ttagaaaagt taaaagattt accttatgaa gaccttggat atgcaaatat
    14761 agaccatcat agagaattaa gaaatgggta tccggaagtt atttattgtg aaggtaagag
    14821 tgatgagcat attttaggga ttattgataa aatgaatcaa aaaagttcta acatattggg
    14881 gacaaggtgc agaaaagaaa cttttctgaa aattaaagaa atctataatc atgctgagta
    14941 cgaagaggcg tctaaaattc ttaagattca aaatcatgat atagagaata taggaaaagg
    15001 taaaattgtg attgctacag ggggaacttc tgatatacca gtagcagacg aagcttatca
    15061 tacagctaag tttttaggta atgatgttga tagaatatat gatgttggag tggcaggtat
    15121 acataggctt ttaaataaaa gacataaaat agatagtgct agagtaatag tagccgttgc
    15181 aggtatggaa ggtgctcttg caagtgtaat tggtggactg gttgatgttc cagtaatagc
    15241 tgttccaact tctgttggat atggagcaaa ttttggagga ttagcagcat tactagctat
    15301 gttaaatagc tgtgcatctg gaatatctgt tgtaaatatt gataatggat ttggagcagg
    15361 atatttagca tcaatgataa ataaactgta aatacattga tataaattaa acaaaactat
    15421 ataataaaat ttaatttcta ttatattttt actattaaaa ttgaggagac atatatgagt
    15481 gaaaaaatac tttattttga tataataagc ggtatatcag gggatatgac ccttgcaagt
    15541 ttattaaatt taggtgtgcc taaggaaatt tttttggagg aaattcataa actaagaatg
    15601 agcgatgaat ttaatataaa catcagctcg aaaactgaaa atggaatagt aggtacaaaa
    15661 gtagaagtaa ttacaaaaga aaaacataca catagaaatt tagtggatat atttgaggtt
    15721 atagataaaa gcactctaaa cgaaaatgta aaatctaaag caaaaaaaat atttatggta
    15781 attggagaag cagaagcaaa agttcatgga actacaatag acaagataca ttttcatgag
    15841 gtaggggcta tagattcaat tgtagatata gtaggagctt ctatattagt tgacttactt
    15901 tgtgtagata aagtttgtgc tagtacagtg tctgttggtt caggatttgt aaaatgtgaa
    15961 catggaatta tacctatacc tgcacctgca actatagaaa ttttaaaagg tgtgccaatt
    16021 aaattaaata atgtaaatgg agaatgtaca actccaacag gagctgcaat cataaaagtc
    16081 ttgtgtgatg agtttgtaga tgagcttgaa ttttctccaa agcaaattgg atatggtata
    16141 ggtcataaga agtttgaagt accaaatata ctcagaactg tattaggtga aaaaaaaaag
    16201 aagaattaat ttatgaaatt agtgcaaaca ttgatgatat gtcttcagaa atctattctt
    16261 atttgtatga aaaaatatta gatgagaaag cattagatat ttatacagag agcatttata
    16321 tgaagaagaa tagaccagca acaaaactat caattttatg taatgaatca gatttagaaa
    16381 aatttattaa aatattactt ttagaaacta gtacttttgg agtgagatat aatgcatata
    16441 atagagaaat tctagagaga aaatttgtaa aattagatac aaaatatgga gtggttacag
    16501 taaaactagg gtattataaa ggaaaactag tcaaggctac tccagaatat aatgaatgta
    16561 aaactatagc aaaaaatgaa gatattccta taaaaaaagt gtttgctgat ataaatttta
    16621 gaataaaaga agaatttagt gaaaacttat tgacataagt aataaatttt gataaattaa
    16681 tattcagcct tattaaataa aagtaaacag aaaaaaatat ataaaatata aactcactcg
    16741 tatatgctca gaaatatggt ctgagagtct ctaccaagat accgtaaata tcttgactat
    16801 gagtgaaatt attataccaa aagacctagt taagtaaatg tgtaggctga gttaaactcc
    16861 ctatgtgttg gctataagat aaaggtattt tggaagcctg tatagtaatt tcacttttgc
    16921 tagaaattat ggaagttact atacaggttt tttgtgtaaa aagatttaat ataatttaga
    16981 ctgtattgtg ttaaaataaa tatataaaaa ctatattata caaggagaga tgtgaattta
    17041 tgaaacatga attagtactt gtaatagatt ttggaggaca atataatcag ttgatagcta
    17101 gaagagttag agaaaacaac gtatattgtg aaatacttcc atgtactgca agcatagaaa
    17161 gaataaaaga aaaaaatcct aaagggataa tatttacagg tggtccaaat agtgcgtatt
    17221 tagaagattc tccaacaata tcaaaagaaa tatttgagct aggagtacca atacttggaa
    17281 tatgctatgg tattcaaata atgtctcatg tattgggtgg agttgtaaga aaaggtaata
    17341 aacaagaaaa agaatatgga aaaacagcta taacttatgg aaaatcatca ttatttgaag
    17401 gaataactac aaatagtgta tggatgagtc atacagattt aatagaaaaa gttccagaag
    17461 gatttactat agttgcaaat actaatgatt gtccagtagc agctatggaa aatgtagaac
    17521 gaaacctata tggagttcaa ttccatccag aggtagaaca ctgtttggaa ggggacaaga
    17581 tacttacaaa cttcttatat aatatttgta aagtaaaagg tgactggact acagattcat
    17641 tcatagaaga taaaataaaa gaattaaaag aaaaaatagg cgataaaaaa gctttatgtg
    17701 ctttaagtgg aggagttgat tcttctgttg ctgcagttct tattcataaa gcaattgggg
    17761 acaacttaac ttgtatattt gttgaccatg gtttacttag aaaaaatgaa ggaaatgatg
    17821 tagaaagaat atttagagag aaatttgata taaatttaat aagagttaat gcagaagata
    17881 gattcttatc taaattaaaa ggagtttctg aaccagaagc taagagaaaa ataataggtg
    17941 aagaatttat aagagtattt gaagaagaat caaataaatt aggaaaaatg gatttcttag
    18001 tacaaggaac aatctatcca gatgttatag aaagtggaca tggaaatgct gctactataa
    18061 agtctcatca taatgttggt ggaatcccag aagatgtaga cttccaagaa attgtagaac
    18121 cattaagaga gttatttaag gatgaagtta gaaaaatagg attagagctt ggtatagaag
    18181 aaggattaat atttagacat cctttcccag gaccaggtct aggaataaga gttataggtg
    18241 atgtaactaa agaaaaatgt gatatattaa gagaagctga tgctgtttat atggacgagc
    18301 ttagaaaagc aggtttatac agagaaatat ggcaagcttt tgcaacatta ccagatgtta
    18361 agactgttgg agttatgggt gatgaaagaa catatgctta tttagttggt ttaagagcag
    18421 ttacttcttc tgatggaatg acttctgact ggtataagat gccttatgat gtattagaaa
    18481 gaatttctaa tagaataata aatgaagttg atggagtaaa tagagtagtt tatgatataa
    18541 cttctaagcc tcctggaact attgaatggg aataaaatat agaatgccaa aaatccacat
    18601 aaatacagga tttgaggtct ctcttacttc tatgttatac tggtttgata ctatcaatat
    18661 ttgccagtat cttatggatt aataagcaaa aaacatcctg ctgaatgata aattcagcgg
    18721 ggtgttttta ttttgtttta gaactccttt gctctgaaac tcgaagtatt taacactgat
    18781 gattttgtta tcttttagtt ccttttgtgt catgctctga aagacaaatt gatacaataa
    18841 aaaagtaatt tcttaatcat tgatggagat atgtatttat gttactcttg atggtaattt
    18901 gagtaatatt gttcatagct ttttatattc acttaattag aaaaccattt ggtataatca
    18961 taaataaact tgatgatata atagaaaaga tttataatta ttagaagtat ataaaagtat
    19021 tttatgtcta tgaaatgtat gtctataaaa catatagatg acttttgtat attaatgtat
    19081 gaatattgat attatagaag aattgagttg tcaatgatac taaatcaaat atttatagaa
    19141 gctggaggga ttatgagaaa taaaataatt ataggaatta ctttttcact tgtattacta
    19201 atgtctggat gtgcttcaaa acaaaccgca gaaataaacg tattagcaac tactgattta
    19261 catggagtta taccctatga gttaacttct tatgtaaaag aagaaagaaa aaaagataaa
    19321 aatctaactt tagtagatgc aggagatttt tttgatagtg gagaagtgtt tggttctagc
    19381 atggatagtt attttagtgc tagatataaa gatggtgata ataacactga aagttatatt
    19441 gagacgccta tttcaaaaga tatgaaagag gtaggttatg atgttgcagt tcttgggaat
    19501 catgaattta tatccaacaa taaattttat ctagataata tgatatctga ttttgagaag
    19561 caaaaaataa atattttatc ggcaaatacg tataaaaaaa atggagacag ttatacaaaa
    19621 ccatacataa taaaaaatat taatacacct gaaggaaacg taaaactagg aattttaggg
    19681 cttactataa aagaagttgg tgagaggaaa cagtggaaag atggaaaact tgttgactct
    19741 aagtctttag aactaaaaga ccaagagggt tacaatggtg aattgtatat gaatgattta
    19801 gtagaagatg ctaaaaagtg ggttgggata atggaaaaag agaagcctga cattatagtt
    19861 gctgtggcac atacaggtga gaaacctaag aaacctaaaa acccaggaaa tagaatacaa
    19921 gacttagctc aacaagttga tggaattgat gctatagttg caggtcataa tcatgttcaa
    19981 attgagcaac atgattataa aaataaatca ggagaaaatg ttatagttac tgagccagga
    20041 aagcatgggg aatgtatttc taagattaat tttaaattag aaaaaaacaa tgataaatgg
    20101 aatatagttg acaaatctag taaattaatt aaatttgaaa aaaatcaaga aagtgataaa
    20161 tttggagaat ttatttcttc aattaataaa gtaagtaaag ataagaaaga aataagatta
    20221 tcagaagtaa agactcctga atgggataaa atatatgttt ttaaatctgg aacatctgca
    20281 gatgatatat ataaaacaat aggatataaa tggagaaata tatcatctcc aaagggtgat
    20341 gacatggtgc agattgttgt aatgaataat aataagcctg ttagttatgt atatggtgaa
    20401 gctgaagtta tgccaattaa tataaaatgt gatgaatcag agtataaaga taatgttgta
    20461 actatttatc ctaataaaaa tgataaattt aaaattgaaa aaggtaaaca agaatatagt
    20521 attaatttaa aacatataca atagaaactt ttatcttatt taagtttaga ttgtacttaa
    20581 aataaagtgt gtattatttt aaaaattgat gataattaaa atcacaacat taaggaatag
    20641 tacacacttt ttatgctaaa aaatagaatg ttaaaaaatt aaacctttaa attaaagtac
    20701 attgcttata ttatccaata ttattttcaa aggttgtgta atatttttat ccttatgtaa
    20761 tattgctaac attttatttt ccatatctat cccatctaat tcaatcaatc gcaatttttt
    20821 atgaaagagt gaatctaata ataaattttc tggcaaaatg gttataccta accctgcttc
    20881 tgcagcttta ataagtgcaa aagaattaac actttcccaa acaggataag ctttttggtt
    20941 tgaaagagat aatacactgt ccaaagtatt gcgaatggca cttccttgct cacgtaacaa
    21001 taacggaaac gtgcataact gtcgtagaga taaggcttgt ttagtattgg tggtggttat
    21061 gcaatcatac ctttaatcca agagaaagta gtaaatcaaa ctggatggat aagtgagaaa
    21121 atgtttacag atattataac tatttcacaa atgacaccag ggccactggc tgttaatact
    21181 tctacttttg ttggacttca aattggaggg ttttggggag catttgcagc cactttaggt
    21241 tgtgtattat gtggtgtagt aatatctcta tttttatata atttttttca aaagcatcag
    21301 aactctactt atatatttga aatattaaat ggtctaaaat cagcttcatt aggtttaatt
    21361 atctcagctg cggctacaat tataattctt gcattatttg ggacgaataa agtgtacttc
    21421 aattctatat ttgagatgct aaactggact gcactaataa tcttttttgt tacactactg
    21481 atagtacgta agtggaaaat taatcctatt gtgattataa taataagtgg tatactggga
    21541 atattttttt atttataagt ttttgctaag taaaggatat ctattttttt atatcttaaa
    21601 tccaaatagc caataaagta aggctatttg gatttttaat aaattctgaa aaaataatca
    21661 gaaacccata taacatttaa attatatcac tggttttcta caaaccaatc ataaacttct
    21721 tggcaaaatt tctcactatc tatatagtaa ctattatgaa cattattaat ttcaacaagt
    21781 ttctttttac attttagtga attataatag ctatcaaaat attgggtttt aaaatatcta
    21841 tcatgaagtg tcttaaagac aagagtaggt atgttgactc catcttttat cttactatct
    21901 tctgcaataa agcattctaa cattgtatta attatctttg tactattttt ccaaatatga
    21961 ggatttttct tttcaaatat tttataaaat tcattatcat agacttcatt ataaccttta
    22021 tatttaggtg gttttactgt tatgtgaaaa ttaggaagaa acttaaagaa aaaatataat
    22081 aatttttatg ttctgtttta tatattattc atattttatc acagataata tactaaagtt
    22141 aattttttta ttgtacagct attataataa ttcttctata atataagaca ttctataatt
    22201 ttatatttaa gttcatgtgt atttaattgt ttttatataa aatgtccagt aatactattt
    22261 ggatttattt ttatttcatc aggagttcca gaagcaacaa tttcccctcc atttattcca
    22321 ctgtctggac ctatatcgat aatccaatca gaagcacgta taacctctgt attgtgttca
    22381 ataattaaaa gtgaatgctg ttcatttaca agttttctaa aagtaattaa caaattttct
    22441 atatctttaa aatgaagtcc agtagtaggc tcatccaata tgtacagcat tcgtttttta
    22501 gtttttgtcc ctaattcttt tgctaatttt atcctttgag attctcctcc acttaatcct
    22561 aatgtatttt gtccaagctt tagatatcca agtcccactt catctaataa ggagagtttt
    22621 ctatatatat cagagcaatt gctaaaaaat gtttttgctt ctgctacttc catgtcaagt
    22681 atatctgcaa ttgtctttcc cttgtattgt atagacagta tttctttttg atatctctta
    22741 ccttgacatt cactacaagt tacccattgg tctgccatat attgcagaat aatttttgtt
    22801 ttacctaatc cttgacagtt tgggcattgc ccctttttgc tattaaagct aaaatagctt
    22861 tcatccaata atttttgttt tgcttgtgga gtttcagcaa atagagaacg aatttcatca
    22921 aaaatattga tatatgtagc aggtgtagaa cggctagatt ttccaatagg tgtttggtcc
    22981 attaagataa aatcatcaat agcatcaaat ccagtaaatt cagtataatt tttatctggg
    23041 atagattttt gtttcaattt ttcttctaga gcaggaagta aactgtgaaa tacaagagag
    23101 ctcttaccag aaccactaac acctgtaata gagcacatac agtttaaggg aatttctaca
    23161 tcaatatttt ttaagttatt tgcatggcat ccttttaagc ttaaccattt acttggtata
    23221 attttatttt gtgaacttat tttattgtat gtgcttaaat atttacctgt tatagagttt
    23281 ggatttttta taatttcttg ggttgtacct tctgcaataa tgaacccccc ttttgtacca
    23341 gcacttgggc ctatatcaat aatataatca gcagacagta ttgtatcttt atcgtgttcc
    23401 acaactataa cagtattacc cttatctctt agctcttcta tcattttgat gattaaatta
    23461 tggtctcttg ggtgtaaacc aatagatggt tcatctaaaa tgtaggttaa gcctactaat
    23521 tcacttccta gctgacttga taatcgtact ctctgcagtt ctcctccaga taaagttgga
    23581 gctgttctat ctgttgatat atagctaaga cctaccttta atagataact aactcgaatt
    23641 tttaattctg ttaagatatc atttaccaaa gagagctcat ttttttgaag ttgatttggt
    23701 aattcacaca accaattcca taactgttct atcgtcattt ttgttagttc agggaagcga
    23761 taacctttga ttgtagtgaa tctagcctca atgcacagca attcgccacc acatgtattg
    23821 caaggaattt tgttcatata ttgatgcaag gtattatttt cagaactaga ttctctaaaa
    23881 agtctttgaa tgtggttgat tgctcctgaa gcaggtcttc taatctttga ttctcgtccc
    23941 tttgattcaa agctaaattc atatatctta tcgtctgtac catataagat tgcatctata
    24001 aaatctcttg gtaattcatt ccaaggtata tctaggtcta tatttttatc ttttgctatt
    24061 gcatataatt cacctaccat ccaattacca tttggctttt tccccctcaa ttttccaaaa
    24121 tagtaagtag ctccatctaa tattgaaata gttgggtctt ttacaattaa ttgaggatta
    24181 acttgatagg taaaacctaa tccattacaa tcgtgacaag ctccagtatg ggagttagca
    24241 ttgaaggttg aagtatttat cttagaaaaa atagtttgac aatgcggaca ataatttttt
    24301 gtattattct ttttttctaa tggaatttta caatatggac agtaactttt tccgattgat
    24361 acaaataaca ttcttaaata atcccaaatg cttgtaagag taccaacagt agagcgagga
    24421 ttttggcttc cttttttctg ttcgatagct atagtagggg ttaatccctg aagtaaatca
    24481 aattccggtt tttgaagctg ttctgaaagt tttgctccat tacttagatt ttcaaaataa
    24541 cgtctttttc cttcttcata aatcgtatcg aaagctaaag aagattttcc actaccactt
    24601 acacctgtaa taacagttat ttgatttttt ggaattgtaa cctcaacatc ctttaaattg
    24661 ttttgccttc ctccttttaa atagatagag cttttattat gacaaagtga atttttaatt
    24721 gatttattta ttgattttgt aaatttgaat ttcttttcta gttcaagagg attgcctaat
    24781 tcttttattt ccgaaatacc taatattttt aactgagaaa agattgattt caaataatct
    24841 tcaggcaatt tttctattaa caaattaagg tcttctattt ttatatttcc gcttgataat
    24901 atttctgcca tttcatctaa taaatttttt tctgctcttg ttaattgatt gtattttttg
    24961 gaacttaatt ctgtccataa atcccatgga agcaattcta aaccaatcat attattaaag
    25021 tcatgtagaa gattaccctt tacagaatgc caacctttcc aatttttatt atatccaaag
    25081 tcatctggat gagcatgtcc ttgccgacat cttatccagg cttctgaacc agttaaaaaa
    25141 ccatctgttt ttccgacatc atgagtgtca aaattaattt gaaaataatt tttttgtatg
    25201 tcatctattt gtgcatctat tcttatccat ctctttgtta gagtatcata gtattctaca
    25261 agccagtggt cttcataagt aagctcactt tcaaaataat tagcaaaacc tgctctcatt
    25321 ctagcctcaa aacctcgata tcttaggagc gatacaagaa gtaaagaata atctctgcac
    25381 attccaacaa gcctttgttt tgggtttctt ggaatagtta gtgggttaga atttatttca
    25441 aatattcttt ttaacatttg aggaattgta cgaagcaatt cctcatcaga ttgttgctta
    25501 ttaaaggata taccatataa ttttccttgg tctccatgaa ttactaggct ttgaaccact
    25561 tttaataact caattggagt ctttggtaaa tcttgatatt tattttcaaa ttttttagga
    25621 tttgtgatga tgctttgatt tttatagtta tttattttca tttatatttc acctctctaa
    25681 atggatgata taccctgaca tggtgtcata tgcaaggaca atttaaattt tttgtccaaa
    25741 atttaaaaat aaataaaaaa tgatacagtg ataaattgta atacaaatat agtttcaaat
    25801 tgcatataat ataattattt aataaaacca ttatctatgg aatgttaatg aaatgttaag
    25861 atggcattct tgaatattaa gaaatagaat actataatat agtgtgaagg atttttagta
    25921 aaaaaatttg taagtgttaa tatacattaa aaaataggtt tataatttgg aggaaaaaaa
    25981 tgaaattatc aaggtataag aaaaatagtt tagttctgtc tataatttta tgttcactta
    26041 ttatttcaat ccttgtaatt actgttttta ataccgtaaa actacacaat atatctaata
    26101 aacaatctaa aaactattta aatgatgtat ctacacagat tgtaatgaat gttgattcta
    26161 aaatcaaatt tattttatcc gacttaagaa taatggcaga ttttattaag caatatgaag
    26221 gtgatagtcg atatgagtat ttatctaaaa gaaagactag ttataagtat tatgatgttg
    26281 gaataataga tttagaaggt aaagctgagt ttttaagtgg aaaaaagttt gacttaaaga
    26341 atactgcttc ctataaaaaa ggaattgaag gtaaagaata ctcagaagta atagagtcaa
    26401 tgggttttgt tctttatagt gtacctatat ttaatgataa tcatgaagta tctagtattt
    26461 taataggtgt atccaataaa gaaagtatga atgagattct taatatagat aactttaatg
    26521 gagaaggaac tattgaaatt attaatagta aaggaaaacc tcttttcata ggaaaaaata
    26581 ctgaattaat aaaagacctt agccataaat ataaggattc tacagatgaa ccttgggcac
    26641 aaaagatgct taatgatttt aaaaatgata aatcagggaa tattacaata acctcatcaa
    26701 aaggtgtaca atgcttattg acttatcatc caattacaag aggtttaaat gattggcatt
    26761 ttcttctcat agtacctgaa gatgctgtac ttggtgaact taataagtta aattctttta
    26821 caataacaat gacatttata attacatgta ttattggaat tataacatta attttatata
    26881 ttataagaag aaaatatgta aatcaaatag aaaatattgc atatttagat agtataacaa
    26941 atggcataaa ttcaactaag tttagtatgc tagtcaaacc tttaatttca gaatcacctg
    27001 atagtactta tatgatgata gcaatgaata taaaagattt taagctaata aatgattgtt
    27061 tcggtagtga aaagggaaat atgacactaa aacatcttta caatattttg tataaaaaca
    27121 ttgatgaaaa tgaagaattt gtgtgccgtc atgatgcaga ccttttctat ttgcttatta
    27181 agaatagacc agtgttagaa gccctggagt tattatataa aatagaagct gacacaaatt
    27241 attttaataa aaatagggaa aatccatact ttttaagact atcagtaggt atttacacaa
    27301 ttgaaaatca taatgaagat ttaattacaa tacaagacca tgcaaataca gcaagaaaaa
    27361 gctttaataa aactcatcaa agtgattttt ctttttatag tgatatagaa agaaaacgat
    27421 taatatctga aaaggaaatt tcaaatttaa tggaaaaagc ccttgaaaat aaagaatttt
    27481 ttatgtgctt acaacccaaa attgatataa aaagtggaaa aattagtgga gcagagtctc
    27541 ttgtgcgttg gaaaaattca gaaaagggca taatttatcc aagtgatttt attcctcttt
    27601 tcgaaaaaag tggatttata tgtaagttag atttatatat actagaagaa acatgcaaat
    27661 tgatttctaa gtggataaaa gaaggtaaag aagtgttgag aatttctgtt aatgtatcac
    27721 gtcaacatct gaatgataaa ttgtttttag aaaaatataa gcatatatgc aataaataca
    27781 atgtaccaac atgtctgata gatttagaac ttaccgaatc tattttctta gaaaatccag
    27841 aagctataga tataatagag gatattcatt ctaatggatt taagtgttct atagacgatt
    27901 ttggatttgg gtactcatca ttaggaatat taaaggattt taaagttgat ataattaagt
    27961 tagaccgttc attctttgtt agcaaaaata atatagaccg tggtaaggta gttattaagt
    28021 ctattattga acttagcaaa aggctaggta tgaaagtaac agctgaagga attgaagaat
    28081 tagaacaagt tgagtttcta aaaaatgtgg gatgtgatta tattcaaggt tatgtttttt
    28141 caaaaccatt attaatttct gactttgaaa attttgcata tgaaaataat aaaattaaaa
    28201 atatccatta ttaaatgtac aagataaaaa atttaggcaa gttatataat taaaaatata
    28261 acttgtcttt tttatttata tatttaataa aaatatattt gcagattttg taaaaataat
    28321 tttcaaatgt tgtaagttgt ggctaaagct ttattgtatt attctgaata tagaaagata
    28381 gttactgtct tagataaaaa ctgaaacata cataataata taaaatttgc tatattaata
    28441 actctaatta ttttggaagg agggaaaata tgataagtgg gagaatgtta ctcatcatga
    28501 attttctaaa aggaaaaaat aagacaagtt acaaggatat tagcaaacaa cttagtatag
    28561 aagaaagaaa agttagatat gatataaata atttaaatat tgttttgcgt tcattaaaca
    28621 agtcagtaat acaaaagatg aacaaaggtg tgttaattat tcctgatgac ttccaagtag
    28681 tatgtataaa taataacgaa tatgtatttt ccttaactga gcgagtatct attatgaaaa
    28741 ttatatcaat gtttaaaata gataatctaa acttagaaaa attgagtaaa gaatttcggg
    28801 tttctagaag ttcaattaaa aatgacctta atgttttgtc aagtgaatta gaaagaaacc
    28861 aaataattat tacttatgat aaatctttta aaataagtgg agaagaagac attatatata
    28921 agcaaagatt gaacatatta aaatcgtatt cttatctttt tggtaaggaa aaatctgatt
    28981 taagtgtatt tgagaagtat ttagtgaatg taattgaaaa aatattcaaa gggatttatt
    29041 tgattgatat atataattgg tctatgaatt tattattgca aatgggatgg actttaaatg
    29101 atgaatcttt tacatggtat gtgtcgaaca ttttcctttt tacatggtat atacattcag
    29161 atgaaaaaca tcctttagaa tctgcttcat taagtgagcc gtgttttgaa aataagtttg
    29221 accaagaatt tgaaacaatt attcaaaaaa agttgactaa ggaacaaatt tttctgttaa
    29281 agagttttgt attttttaca aataaatatg caagtttaaa cgaggatatg gatttgatta
    29341 gtacagaaat tatagttcaa gacctaattt acaatatgtc agaaatatta gagattagct
    29401 ttaaagaaga tatgattctt tataaaggat tactcaatca tattgctcca ttgatagaga
    29461 gaatcaagca gaatatacag atttatgagg aattacttca tgtaatacca gataaatatc
    29521 aatatgtact tgaagctact aaggtttcta ttcaaaaaaa cagagtttta agtcaaataa
    29581 agaatgaaaa tgaaacaata cttttggcaa tacatttttt aggaagtata catagaaatg
    29641 agaggaataa ctacaaaaat atattgttag tttgtggatt tggatatggt gtaattgcta
    29701 tgttaaagga tagtttagta aatgattacc agataaatat agtagagagt attccatcat
    29761 ataagttaca agattataaa gaatgggaaa gtattgactt cattataaca acatcaaaga
    29821 tagtaataga cttgcctaaa acaattattg agataaatcc atttttacaa gaagaggatt
    29881 atttgaagtt tgaagaaaaa gggataaaaa gaaagaacgt cttaactaat tatatgtcaa
    29941 taaaaaaaag gttggacttt ttagaagaag atgaccaaaa aagagtatta agtattatat
    30001 gtcaagaact tggatacagt gatgagagaa tattatttag acaacttaat ttaagtgatt
    30061 taatagggat tgataccata aggataattg atggagatat gaaatgggaa gatgctgtaa
    30121 aatttagttc aaacatactt gctaattgtg attttgtatc agcacaatat gctgagaata
    30181 ttatagatat tttaaaaaat gtgggttttt atgctgtaaa agataacgaa tttgctttac
    30241 tacatggcaa tgatagttca ttagtcaaag taagttcgat aagtttatta atatgtaaag
    30301 aagcagttag ttttggagat aaaaaggtta aaatcatatt ttgcctagca agcagagata
    30361 aaaaagagca aattcctgca ataattaatc taactaaaat ggtttataag actaatttta
    30421 ttgctatgtt agaaagtgca aagacaaaag aagaagcaga agttttaatt cataaatatg
    30481 agaaggaggt cactgtatga ataaattagt taatgtaaaa tgttcattta aaatacatgt
    30541 taattcaaaa gaagaagcca ttatatcgtt agttgatgta gtgtcaaaag aaggatattt
    30601 aatagataaa aatcaatttt taaaagatgt tttaaagaga gaagaaacat tgtcgactta
    30661 tattggacat ggtataggtc ttcctcatag tcaaagtgta ggtgttaaaa actcttgtat
    30721 tacaatagga aagcttgata ctcctattga atggacagaa gaaggagaaa aagtggattt
    30781 aatatttttg atatcagtaa caaaagacaa tgaaaataat cttcatttaa agattttatc
    30841 aaaattagca cgtttattga tgcatgaaag ttttagaaat caaattagat gttcagatga
    30901 acaaactgtt tataatctta taaaagaaaa aatagaggag gaagattaaa atgagaattt
    30961 taaagttttt gcaaaaacat cttatgacag caacatctta tatgattcca tttgtggttg
    31021 caggaggaat attatttgct ttatcagtaa ctttaagtgg acaagctgct gttcctgaaa
    31081 caggctggtt ggctaaactg aatcaaattg gagctgctgg cttggcttta ttcattccta
    31141 tattaggtgg atatatagca tttagtatgg cagataaacc aggattagct ccaggtatga
    31201 taggtgcata tttagcaaaa gaagtaaatg caggttttat aggtggtatc atagcaggat
    31261 ttatagcagg atttgttgtt ttacaattaa agaaaattaa attagctcct acaatgagaa
    31321 ctttaggttc tatatttatt tatcctttag ttggtacatt gattacaggt ggtataattg
    31381 tatttttaat tggagaacct atagcttctt ttatgacatg gatgacaaac tggttaaatg
    31441 gaatgtctgg tgtttctaaa attcctttag gtggaatctt aggaggaatg atagcctcag
    31501 atatgggtgg ccctataaat aaggttgctg ctacttttgc ccaaactcaa gttgacacac
    31561 ttccatattt aatgggtgga gttggcgttg caatttgtat acctccaatt ggtttaggat
    31621 tagctacatt attgtttcca aaaaaattta gcaaggaaga aagagattca ggtaaagctt
    31681 cactattaat gggatgtgtt ggaattacag aaggagctat tccatttgca acatcagatc
    31741 cagttagagt aataccttgt attatggctg ggtcgattgt aggaaatatt atggcttttt
    31801 tattaggatg tttaaatcat gcaccctggg gaggtctgat agtgttacct gtagttgaca
    31861 ataggttggg gtatattgca tcagtattaa ctggagcagt agttgttgcg gtattaatga
    31921 aattagtaaa aaaagacgtg aaagaagatg aagaaattga agaagattta gatgaatcaa
    31981 ttgaattaat atttgaagaa ttataaatat aaatggaaag aatttgtata aaaaagtaaa
    32041 tataaaacta taaatttaaa ggagataaga atatgaaaat tgtagcagta acatcttgtc
    32101 ctagtggagt agcacatact tacatgtcgg ctgaatcact tgaattatca gccaaaaaat
    32161 ttggtataga aataaaggtt gagactcaag gaagctctgg aatagataat gaattgtcac
    32221 ttaaagatat tgatgaagca acttgtgtaa tattgactaa tgatgttgag attagaaata
    32281 tggaacgttt taagggaaag aaggtagtaa gaatgagtgt atctgagata attaagaaat
    32341 ctgatgcatt aattaagaaa ataaaagata cttttcaata aaaattgatt tattttacat
    32401 ataactcaaa gatgttttta aagaaatttt tattaaaagc atctttgaaa atatcttaaa
    32461 aatagaataa aaggagatat agtcatgaaa aagttaccaa taaagactgt cgtacaaaga
    32521 ttactaaact tacaagaaga ggggaaaagt gcaactcttt taggaattgg tcctatgtca
    32581 ccaaacttgt tacaagcaag ttttgaacta gcaaaagatg atgattttcc tttaatgttt
    32641 atcgcaagta gaaatcaagt agatgcagat gaactaggtg gcggttatgt aaatggttgg
    32701 aatcaggaaa cttttacaaa agatattaag aaggttgccg acaaagttag atttgatggt
    32761 ttatattatt tatgtagaga ccatggtggg ccatggcaaa gagataaaga gagaaatgac
    32821 catttgcctg ttgatgaagc aatggagtta ggaaaaaaat cttatttagc ggacattgaa
    32881 gcaggttttg acctattgat gattgaccct acaaaagacc catttgaaat tggaaaagta
    32941 attccactag atgtagtact agaaagaact gtagaattaa ttgagtattg tgaaaacgag
    33001 agaaaaagac ttaatctacc agacataggt tatgaggttg gtacagagga aaccaatgga
    33061 ggtctgactt caactgagac gtatgaaaca tttataacta gactaaaggt tgaactcgat
    33121 aatagaggtt tgccaatgcc aacatttata gttggtcaaa ctggtacttt aactagaaag
    33181 acagaacaag taggaacttt taattttaga aatgcttatg atttagctca aatggctaag
    33241 aaatatggag ttggtttaaa agagcataat ggcgattact tagatgatgt aactttatta
    33301 gaacatatac catctcaaat tattgctaca aatgtagctc ctcaatatgg tactgaagaa
    33361 actagggcat atttaaaatt agcagaagtt gagtgtaaat tagaaaaaga gggcttagtt
    33421 gagaaaactt ctaatatcag acatgttctt cttgttaatg caattgaatg tggacgttgg
    33481 agaaaatggg tagtaggaga acaaaaaaat ctaactactg aagagatatt taaggatgaa
    33541 gttttatcta atgaaatact agatattgca ggacattata catttaataa tgatgatgtt
    33601 aaaaaagaaa tagaagtgct ttatgataat ttaagtaaga ataatattga tggtcaaaga
    33661 tttgttgtag accatattaa acgtcctttg cgtgattatg cagaatgcta taacttaaaa
    33721 ggtgttacta cgaggatttt aaataaataa taggacttat agtggtaagt attgttatac
    33781 taggtattta tagttataat tatacttacc actattaaat ttaggaggac taatattttg
    33841 attaatttaa tttttgatgt agatgatact ttatataatc aattaacgcc tttttacact
    33901 gcatataata aagtgttttc ttcaataaag gatatttcta ttgaagattt atatatgagt
    33961 agtagaaagt atagtgatga ggtatttcat atgactgaaa atggagaaat gccaatcaaa
    34021 gaaatgcata tttataggat aatgaaagct tttgaggagt tgggaaattc tattacagaa
    34081 aaagatgcac aaagttttca agatgagtat atatatcaac aatcacaaat tacactaata
    34141 ccagaagttg agaggatttt aaatttttct aaagaaagaa atattaatct aggaattata
    34201 actaatggcc catcaaacca tcaaagaatg aagttaaagc agttaaatat tgaaaactgg
    34261 gttgataaaa gtaatatttt tatttctagt gaggttggat tttctaagcc agatactaat
    34321 atttttagag tagctgagaa tgttatgaat ttagatagag aaaatacata ttatgtgggt
    34381 gattcttata gaaatgatgt attaggtgct aaaaaagctg gttggaagag tatatggttg
    34441 aaccatagag gacatgaggt agaagagttg ttttacaaac ctgattttgt aattttggaa
    34501 cataaggatt tgatatcttt atttataaaa atatgtagta aaaaatgtta ataatagaat
    34561 tgtataaaac aattaaaata tgtgtaaaat cataaattta agttatttac aatatgtttt
    34621 atattaagaa ttgattattt ggagaaatac tttaatatca aaaaagatat taaagtattt
    34681 tttgatttaa aagatttttt atattaataa tataagtttt aaaaagtatt gtccgata
//
LOCUS       DAEMHZ010000030        34649 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000030 DAEMHZ010000000
VERSION     DAEMHZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.030"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            77..751
                     /locus_tag="KPK72_001322"
     CDS             77..751
                     /locus_tag="KPK72_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488554.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            739..1899
                     /locus_tag="KPK72_001323"
     CDS             739..1899
                     /locus_tag="KPK72_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2488555.1"
                     /translation="MDKINNMSLKKTFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNTVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSIFIENNSTNSAKITAKFSTRN"
     gene            2116..2643
                     /locus_tag="KPK72_001324"
     CDS             2116..2643
                     /locus_tag="KPK72_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG2488556.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2929..3597
                     /locus_tag="KPK72_001325"
     CDS             2929..3597
                     /locus_tag="KPK72_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488557.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3600..6068
                     /locus_tag="KPK72_001326"
     CDS             3600..6068
                     /locus_tag="KPK72_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488558.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIRKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVELIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6308..7732)
                     /locus_tag="KPK72_001327"
     CDS             complement(6308..7732)
                     /locus_tag="KPK72_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488559.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7751..8443)
                     /locus_tag="KPK72_001328"
     CDS             complement(7751..8443)
                     /locus_tag="KPK72_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488560.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8498..9274)
                     /locus_tag="KPK72_001329"
     CDS             complement(8498..9274)
                     /locus_tag="KPK72_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2488561.1"
                     /translation="MIKLLSSEWLRAKRTAVQGLTLCMPIIFSLCVVAYLTIKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAETISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9271..10041)
                     /locus_tag="KPK72_001330"
     CDS             complement(9271..10041)
                     /locus_tag="KPK72_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2488562.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTVLEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10038..10742)
                     /locus_tag="KPK72_001331"
     CDS             complement(10038..10742)
                     /locus_tag="KPK72_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2488563.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(10997..11509)
                     /locus_tag="KPK72_001332"
     CDS             complement(10997..11509)
                     /locus_tag="KPK72_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488564.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11835..12092
                     /locus_tag="KPK72_001333"
     CDS             11835..12092
                     /locus_tag="KPK72_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488565.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12235..12861
                     /locus_tag="KPK72_001334"
     CDS             12235..12861
                     /locus_tag="KPK72_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG2488566.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13111..13890)
                     /locus_tag="KPK72_001335"
     CDS             complement(13111..13890)
                     /locus_tag="KPK72_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2488567.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLGK"
     gene            complement(13958..14419)
                     /locus_tag="KPK72_001336"
     CDS             complement(13958..14419)
                     /locus_tag="KPK72_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2488568.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14395..15072)
                     /locus_tag="KPK72_001337"
     CDS             complement(14395..15072)
                     /locus_tag="KPK72_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2488569.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15305..15691
                     /locus_tag="KPK72_001338"
     CDS             15305..15691
                     /locus_tag="KPK72_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2488570.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15694..17493
                     /locus_tag="KPK72_001339"
     CDS             15694..17493
                     /locus_tag="KPK72_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG2488571.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLLSWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17848..19716)
                     /locus_tag="KPK72_001340"
     CDS             complement(17848..19716)
                     /locus_tag="KPK72_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2488572.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLGDG
                     FAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVAVK
                     QGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE"
     gene            complement(19721..21385)
                     /locus_tag="KPK72_001341"
     CDS             complement(19721..21385)
                     /locus_tag="KPK72_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG2488573.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDVNLLNKTVRLKPFHAVLLKAR"
     gene            complement(21399..22220)
                     /locus_tag="KPK72_001342"
     CDS             complement(21399..22220)
                     /locus_tag="KPK72_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2488574.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIFMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22319..23083)
                     /locus_tag="KPK72_001343"
     CDS             complement(22319..23083)
                     /locus_tag="KPK72_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2488575.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23080..23829)
                     /locus_tag="KPK72_001344"
     CDS             complement(23080..23829)
                     /locus_tag="KPK72_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2488576.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIVKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24111..25163)
                     /locus_tag="KPK72_001345"
     CDS             complement(24111..25163)
                     /locus_tag="KPK72_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488577.1"
                     /translation="MNVINVFNSNLKRNINKRIVFLVTLLFPIIIVVLGVLANYISKP
                     SFNIGILDEYKNECILKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFELYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQLFISFVTYIEERDGSFFNMNYICILTFIIIILALL
                     AMKFTQRSKYSSNRNY"
     gene            complement(25163..25903)
                     /locus_tag="KPK72_001346"
     CDS             complement(25163..25903)
                     /locus_tag="KPK72_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488578.1"
                     /translation="MRCFLTLFKLGIKRRSKDFFILFYNIVFPVIIIVLLGYLTSKSY
                     GKEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTTVFSIYNLITLVVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFMTILTIKFFKKEVFI"
     gene            complement(25908..26666)
                     /locus_tag="KPK72_001347"
     CDS             complement(25908..26666)
                     /locus_tag="KPK72_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488579.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKYALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPQILIM
                     DEPTVGIDPQSRNKILEAIKGINNNGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLKFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(26771..27493)
                     /locus_tag="KPK72_001348"
     CDS             complement(26771..27493)
                     /locus_tag="KPK72_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488580.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENVDKIILKLQNK"
     gene            complement(27594..28199)
                     /locus_tag="KPK72_001349"
     CDS             complement(27594..28199)
                     /locus_tag="KPK72_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2488581.1"
                     /translation="MKIKENVFMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEEIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            28378..29016
                     /locus_tag="KPK72_001350"
     CDS             28378..29016
                     /locus_tag="KPK72_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2488582.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            29148..29810
                     /locus_tag="KPK72_001351"
     CDS             29148..29810
                     /locus_tag="KPK72_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488583.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLVLLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSILQSRINAVLRRTYSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            29810..30991
                     /locus_tag="KPK72_001352"
     CDS             29810..30991
                     /locus_tag="KPK72_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2488584.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLTVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESILLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     KASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(31172..33832)
                     /locus_tag="KPK72_001353"
     CDS             complement(31172..33832)
                     /locus_tag="KPK72_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488585.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSGNYHADAPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAKVVYGSDNYKDQRDIEVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVGRLRSNRLIGLFKVNNREDIPRVREKLESICNKDYGSGDYSIEDVL
                     ESEKQDASGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(33829..34512)
                     /locus_tag="KPK72_001354"
     CDS             complement(33829..34512)
                     /locus_tag="KPK72_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488586.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT        12337 a         5556 c         4245 g        12511 t
ORIGIN      
        1 gaatttgata atttattgag atttttatac taaaatatac atactataca aacttaattt
       61 agaagggagc attcttatga acaataaaat actcgtcgta gatgatgagg aaggtataat
      121 aacactctta aaagattact ttgaaatgaa tggttatgag gtttacactg ctcaaagtgg
      181 aaaagacgca ttaaaaaaaa tatccaaaaa tccagatatc attctacttg atataaacat
      241 gcctgaaata gatggaattg aagtttgtaa tagaattaga gattatgtat catgccctat
      301 tctatttctt acagctagaa ttgaaaatac tgacaagata aatggttttc gtgctggtgc
      361 tgatgattat attgtaaaac cttttgattt agacgaactt ggagctagag tatctgccca
      421 tttaagacgt gaacaaagga agtcaaatca aactaaaatt aaattttata atgatttaac
      481 aatagattac gaaaaaaggt taatttgtat atataataat cagatttcac tttcaaaaaa
      541 ggaatttgat atagtagagc ttctctctat gaatcctgga caagtatttg ataaagaaaa
      601 aatatatgat agagtctggg gatacaatag caatggaaat agtgatgtta taatggaaca
      661 cattagaaaa atacgaatca aactatcaaa acatactctt gaaaattata tagaaacagt
      721 ctggggagtt ggttataaat ggataaaata aataatatgt cattaaaaaa aactttcttt
      781 gttcttactt tatcttcact aattatagca tcagtactta ctactttcgc atacatttta
      841 cttaataatc tatataattc tatacaagat aagtatttaa aaacacctat aaatacaggt
      901 attataacca tagttcaaaa caatgtgaac caatttagtg attatgataa aacattactt
      961 aatataatca acatacttca gcttgtactt ccgcttatct tttttatagg acttttatta
     1021 ttagctgata taatttttta caaggtaaaa ttaaaaacac ccatagaaat actcaataaa
     1081 ggtgcaatgg aaatatccaa caacaattta gacttttgct tagagtataa taataatgat
     1141 gaattaggta atctatgtaa tgcttttgaa aaaatgaggt ctgaactaaa taaaaacaac
     1201 ataaagatgt ggactatgat taatgataga aaacaactta atgctgcttt ttctcatgat
     1261 ttaagaaatc ccctaacagt tctaaaaggt tactcaaact atttaacaaa atacatacca
     1321 acaggtaagc ttagtgatga gaagatttta tcaaccacac aacttatgtc tgagcatatt
     1381 aacagaattg aatactatgt agaaaatatg agtaatgctc aacgattaga agatttagtt
     1441 gtttcaaaat ctatgtcgaa tataaacaag tttatagaaa acttagatga aaatatatct
     1501 atcattgcta aacaagaagg aaaatctttt acacttaaaa gtcaaataaa taatataaat
     1561 ctattttttg atgaaaatat cattcttaga gtcgtcgaaa atataatatc gaatgctttt
     1621 agatatgcta gaaatactgt atccatactt atatatcttg aacaagaact ccttaccttt
     1681 gtaattgaag atgatggtat aggatttagt aaggaatctc taaaattagc tttaagacct
     1741 ttctatagag ataagacttt aaatgacagc aatgcacact ttggtatggg tctttatatc
     1801 tcaaaaatat tatgtgaaaa acatggtggc tctattttta ttgaaaataa ttctactaac
     1861 agtgctaaaa ttactgctaa attctcaaca agaaattaaa atgattttgt aagctttcac
     1921 tgctattata taagctttgt tagtttagaa tttataaaat gtatttcaat ttacaaccaa
     1981 tatatatact attttgtaga agttgtaaga gttgcttcaa ttataatatt ttggcaaaag
     2041 gcatttgaaa taataataga aatatatatt taaatatgta gaatagtatg aaattagaat
     2101 tttggaggtg attatattaa aagttttgct aataatatta cattctctcg tattgtgttt
     2161 gcattgctaa tgcttagttc tacattctgt tcactgcctt tctggatatg ttatttttgg
     2221 tgtggaatta gtgatatact tgatggtctt attgcccgaa agttgagaca acaaagtgga
     2281 ataggtgcaa agttagatag tattgctgat ttcatttttg cggttacaat tttggttact
     2341 gtcggaaaaa atattgctct tcccatgtat ctatggtggt gtattgttgc aatagcattg
     2401 ttacggttga ttagttacag tattggtttt tttaagtatc gcacattttc atcacttcat
     2461 actttgatga ataaaatcac tggaatttct atctttgctt ttccattact ctatatttgc
     2521 tttggtcttg atgttgcagg aattatgata tgtatcgtgg cttttctttc ttcttttgaa
     2581 gaaatagcaa ttatgataaa atcaaaagaa ttaaatcgtg atataaaaag tatatttatg
     2641 tgacaactta gaatttgtga caaaaataat ttatataata atgaggaagt aaaacataaa
     2701 aaattatcca caagtttatg tgtaaaatag ttttaaagca atagagttgt attgtaaagc
     2761 ctttagttca taaattacat tttaaattaa aaatgatact gaaactgctt atcagcactg
     2821 cgaattatcc gcaaatagtg aagacttttt agatttaagc taaaagttgg gaaaaaattg
     2881 agttttaaaa cttacactct tcttatatag aaaaggagag tgttttttat gattataaaa
     2941 gcaaaacaat tatcaaaaat atatggttct aataacaata aagttatcgc acttaataat
     3001 gtaaatttag aaataaactc aggcgaattt gtttctgtaa ttggaccttc tggaagtgga
     3061 aaaagcacac ttttacatat tttaagtgga ttagataatc ctacctctgg acaagtgtta
     3121 cttgatgata aagatatata taaacacact gaaaaagaac tatccgcact tagaagaaaa
     3181 tcctttggat tcgtctttca acaatttaat cttttacctg ttcttacagc atctgaaaat
     3241 atttcaatgc ctgtacttct agataaaaaa caacctgata aaggctattt aaatgaaatt
     3301 tcatcattac ttggaattgc agatagactc aatcatcttc cccatgaact ttcaggcggt
     3361 cagcaacaaa gagttgcaat tgctagagca cttattgcaa aaccagatat aatatttgct
     3421 gatgagccta caggaaactt ggacagtaaa agtggcagtg aagttatgaa tcttcttata
     3481 aaaacttcaa aacagtttgg aaagacttta gtggtaatta ctcatgatga tagaattgca
     3541 aaacttgctg atagaaaaat ttcaattatt gatggtgttc ttatggaggt gaaatagaaa
     3601 tgaagaatta cctttcactt tcaataaaag aattaaaaag ccagaaactt atgactacat
     3661 tcatcattat agcaatagta ctatcaacta ttatgacaac tgtggttgga cagtcaattg
     3721 gtatcctaca aaatttaaga attgaacaag cccgttcttt taatggtgat agacatgtat
     3781 cctttcacca actcactaaa aatcaagttg atgacataag gaaagatgat agagtttatc
     3841 aagctggtac ttcaataact attggcagta gtaaaataaa ggattctggt atctctgtac
     3901 ttgtaaaaga gtatgataaa acaggacttt ctaactaccc aaaattaatg aaattaaaat
     3961 ctggacattt gcctaaagat aaaaacgaaa tagccttaga tgaaaacaca ctaaagctta
     4021 tgggtataaa accaagacta ggagtgacta tacctatgaa tttagatatt tcacttttaa
     4081 atgataccat accaccatat aattacacag ctaactttaa attgagtggc attttagagg
     4141 atgattatac aggatatgta agtggtatag taaatggtat tgtaggtaaa ggtacatcag
     4201 aaaatctatt acctaagaga tatattttat cctctttgga ttttaaaata aaacagcaag
     4261 aaaagtttca agaaatagta aatcaattag ctaaaaaaat aaatctttct cacaattcaa
     4321 ttcaatataa ttggatttac ttaaatgctc ttaagataca gtttgaaaaa gatgaaaata
     4381 gttctaatag tgatggtata tctatgatta tattggtatc tttatttgtt gccttattgg
     4441 ttttactggc ttctggactt gtaatttata atattttaaa aatctctgtt acaaagaaaa
     4501 ttaaagagta tggatgttta agagcaatag gagctgaacc aaaccagata tataagatag
     4561 ttattttaca aatcttaatt ttatgtactg ttgccatacc tattggagct gtaattggaa
     4621 ttatatcttc taaaggcata actggaatgg tcactaatat cttaaatcca gatatacttc
     4681 tagcaaatga taataaagaa attactgagc ttatacataa aaatacaaca gcatatatgt
     4741 tcccattagt attaagcact aatgtatctt tgattttttc atttatttca gcattaccat
     4801 cagcaatata tgcttcacat gtctctccca aaattgctat ggttggtagt actaccaaaa
     4861 tcaaaagaaa aattaaaaga gaaaaaacaa taaaaaactt tgaaagacat ctagcatggc
     4921 taaacttaaa aagaaataaa ggaagaacta taataactat actatcatta tttatgagta
     4981 taacagtatt cgttgcactg agtgagttct ctaatgtatt agatgtatct aggtcagtaa
     5041 gtaatttaaa agaaggagat ttttccttaa caaatgaaat atctggtttt gataaatcaa
     5101 cactagataa aatagctaaa atgaaaaacg tcaatcgcac ttcctttata aagtactctg
     5161 aatacaaaca gggtgaaata gatacagata taaattttga aaatagtggt gagatgttaa
     5221 aaatcattgg aattgatgag caaactttaa aagacttaat gccttctatt acagattcta
     5281 tactagaaga ctttaaaaat ggttctatct gttttataaa aaatccgatt gctatttcta
     5341 caccaggtgt aaaaactaga catacaaatt taaaacctaa agatagtatc acaatcaaca
     5401 aaaaacaact agatatatat agtacagtag ataaaatgtt ctttttacaa ggtaatggct
     5461 ggataaatgg tgttgatgtt attgtatatg attctgttta taacaccttg acaaataaaa
     5521 acaagttcaa ccagataaat atttatgcta aagataaatc aaaattagaa caaataagat
     5581 tgtctattga acagatttgt gaaaataatc ctggctctca ttggatatca tatattgaat
     5641 cagataaaca acttaaagag agttttaaac aaatagaatt tctagcatgg gcagttatat
     5701 tatttgtgga attgattggt actttaaaca tcattaacac aactcataca aatataaata
     5761 ctaggactaa tgagattgga gttaaaagag ctattggtat gagtaatagt agcttatata
     5821 aaatgttttt atgggaaggt gtttattatg gaatttttgc tgctatattt ggttcaattg
     5881 ctgggtatgc cagtgctatt attataaaca tggcaactat agaaaagctt gatttcacta
     5941 atataccaat tacttcaatt ttacaagcta ctattatttc tgtacttgca tgtattatag
     6001 ctactcttat tccgttaaga aaagttaaaa agatgaatat aattgactgt atagatatca
     6061 atttataact attttacaga taataatata aattaaatta tcaatgactg tctctatttt
     6121 ctttcaaatc taactaataa atctttttaa ctataatata agctttaaaa aaggcctgtc
     6181 tcaaaataga gataagcctt ttttcattgt cgaaataatg aaaatatatt taacttcaca
     6241 aaaaaagagt gcctcacgaa ttaaaaattt cgctttgaga cactcccctt taatttaaaa
     6301 tatatagtta aatgctatgc caaaatttca cacatgctcc acctgatttt gaattttcta
     6361 ttttaacaga acctccaagc tgttccacta attgtttgac aatgtaaagc ccaagaccag
     6421 aatgtccacc ttttgtctgc ctagcttcat ctcctcgata aaacttatca agagcctttt
     6481 ttaaatcttt agagctaaac cctctaccag aatcacaaat ctcataagaa atacaatttt
     6541 tttcatcctt tacagtaata ctaatattac cacctgatgg agtgtattgc agactgttgg
     6601 atatgatatt atcaaaaatt cgtgtcaacc tatccacgtc gatttgaatt ggggattgta
     6661 tattcccctg cattttcaat ataagttcaa cttccttttg atgagcctgt aattcatagt
     6721 cttgcacctt ttgcctcaaa aactcaggta aatttatagg aacaagattt aattgtatat
     6781 ttgacttttc taagtcagac gtatattgca tcttttggac aagagcagca cttttttctg
     6841 tattttctct tatcactgta agatacctat gaagtttttc attgtcatct gtattattac
     6901 caataagtgc atctgtatat cccaaaataa tagaaagagg agattttaaa tcatgtgcta
     6961 gagcttccac catttcaaca cgttcctgct ccatcttcca ctgtgcagat aaagagcctt
     7021 ttaattcgtc tttcatttca gaaaaggcac tacaaagttt accgagttca ttatctgaat
     7081 aatagtcgat ttcaaaatca aggtcctttt cttttatctt cttggacgca tcaatcaata
     7141 gatgtaatgg ttgattgata ttctttacaa agcgcttaga aagccatcta gtaaaaccca
     7201 cgatatatag aaatggagaa aacagagcca ttataataac tgcaaataca aatctccctc
     7261 taatatttgc aaaggtgatt ttcacttggt agaatagaat aacagctccc tcaatctttc
     7321 cattatcccc tttgattgga acagtatgga tataaccacc tttacgaaca gttttattga
     7381 taaaattatt aaataattct tcttttgttt taaatagttt tttggggtta gtaccatata
     7441 aaatattgcc attattatcc acaacttgat atagcatatc atccccacga atcgttcttt
     7501 ttaaaccttc ttcattcgat tgtgataaca aagttatgtt tttttcattg atatattttt
     7561 caattctagg aacttgtcgc tcataatagt ttggtggata gatatcctta tttaaggaat
     7621 gaatatacaa atagagagca aatacataag taatcactgt ggcaactata ctggctatta
     7681 taataaaaat aaaagtatgt ctaaattggg taataaggga ttgatttttc atttaataac
     7741 ctctctctct ttacatttta ttccacttat acccaatacc ccaaactgtg gaaatatatt
     7801 ctttttctgg tgtcacagca gaaaacttag ctcgtatttt ttttactcgt tcaacaacag
     7861 ttgaggaatc accttcagaa tcacaccccc atactttttc atagatttgt tctcttgaga
     7921 aaacttgtcc tgcatggagt gctagtaatt caacaatatc atactctatt tttgttaaat
     7981 caatattctt accatcaatt ttaattgcac gttccttcat atataagcat aacttcccaa
     8041 aatacaaagt agatctcttg ttctcttcat tgatatactg tgaacgcttt tccctacgca
     8101 gattagcttc aatccttgcc ataagttcac gcagtccaaa tggctttgtt acataatcat
     8161 cccctccaaa ggtcaaccct ttgattttgt cggtttctga ttgctttgca cttaaaaata
     8221 taattgggca taaaacatca tctctaattg cctgacatac ctcaaatcca ttcattttag
     8281 gcatcataat atccagtata ataaggtcag gctcctgttt tgataactct atacctgcct
     8341 gaccatcata agctatcaac acttcatgtc cttttgcctt caattcatcc tcaagtagca
     8401 ttactaggtc catttcatca tcaataatca atattttact caatataaca ccacctttct
     8461 cttatgggaa ggtcagtatt aaaacaatat ttgtctacta ataacttttc cttccctccc
     8521 acattttaaa ccaaataacg cctcctatca agaatataat caaacctatt gctactacta
     8581 taagctctat actagctgtt tgcatcactt tagagtaaaa tgcctcagtt gaaatagttt
     8641 cagcttcttt aataaaataa atccctggaa gcatacctag ttttactggc catgtccatg
     8701 gaacaaatgc ccatactttt tcaccaacgc tagttaatcc aagtattgct gccatcaaaa
     8761 ttccaccgat actaatacca atagatgcac ccatacccca tgcaaaactc acccaaagat
     8821 gaatagctaa tagtggaagt gtcccaatca ccacaagtcc tgctgctgat aggaataacc
     8881 atattgttgc tccagatgga actgcaatac tcattcctat gcagagtatc aacgtagcaa
     8941 taaatgtgca tatagttagg caaaacagaa gaaaaagaaa ttttcccaga tataatctaa
     9001 cacgagaaat acctacacat aaaaaaccat taaaatttcc agcaagctct tcctcctgca
     9061 ctataaatcc tgctaaaata cctactccaa taggaatgat gaatacagtc catactgtaa
     9121 aaaatccttc aaaagcaaat tcttgtgtac ttccagattt aattgtgaga taagcaacca
     9181 cacataatga aaaaataata ggcatacaaa gagtaagccc ttggacagct gtcctttttg
     9241 ctcgaagcca ttctgatgat aatagtttta tcatttgtca tccctcctat tgaaccaagc
     9301 tgcagtaagt gctgttaata ctataaacac aaccaaagaa acaacaatcc caataggaat
     9361 cactgaagta ttcaaaagtg ggtctccagc ttctaataca gtaccgttag gatgtatgtt
     9421 gatgattggg cataccattc taactgccca actccaaggt actgcaatcc aaaatggttt
     9481 tgtcgcagca aggacaccaa atatcatgcc taaagctcca actcccatac ttaggaacat
     9541 acctttccat gtagctagcc atagctgaag tggaagtata gcaagagaaa caacccagca
     9601 tacaatactt gcccctataa tctgtccaaa aggaactgga ccagctttca atattaaacc
     9661 agttataatt gttacgaata ttaaaactag cgttgaaata aaactgtaga ctgccatagc
     9721 tataacttta ttaatccaaa gtgtcatagg agacacattg tgtgtacgta aagcacgata
     9781 atttccagct tttttttcct ggaaagctac caaagtagca aacaatgcaa atccaatagg
     9841 cagaaataaa aatgaccacc agttaaaaat taatgctaaa agactttgcc acgcccaaga
     9901 agttgtttga gataatggat tttgcatgag tgttgattgc attataaatg catatgctgc
     9961 aaaagacact gggaaaaaaa caataagttt acccataaat gttcttttat actttaaaag
    10021 ttctgattgt aaagcattca tctgttttgt cctcctttaa gaataacatc tgtaaaaaat
    10081 tcttctagat tttcattagg gtcaggcatt ccttgaaata gtagtcgtcc tccatttata
    10141 attcctatat tatctacaac ttgagcaacc tctgatagaa tatggctaga aagaattaca
    10201 gttatacctt tttttggaaa agaagcaatc aattcacgca attcttgaat gccaaagggg
    10261 tcaagaccat tggttggttc atctagtatt aacaactctg ggtcactcaa aagtgcaata
    10321 gctattccta acctttgttt catacccata gaaaattgtg aagctctctt tttacctgta
    10381 tttttcaaat ttactatttc caacacttca taaattttat ctttaggtat tcccaatatt
    10441 tttgtatgta ctaacaaatt ttcctctgct gtaaggtttc catacaatgc aggggattct
    10501 attaaagaac ctatttttgc aagagattct cgtttccaag gctttctatc aaaaataata
    10561 cgaccatctg ttgggcgaag aagaccaaca agcattttaa gtgttgtgga ctttcctgct
    10621 ccatttggtc ctaaaagtcc atatatacta tttcgttcaa tttgtagaga aacattgtta
    10681 actgccattt gttctccata tctttttgat aagttttttg tttcaagaat taaatctttc
    10741 atattaattt ctcccttctt ataatacctt aaatctatta ctaagagaat actaacatga
    10801 aaaaataaca attctataac acctactaat tttaataaaa attaaaataa aaaatagagt
    10861 taccaatttt attttggcaa ctctatttaa gtttatgttt tacagttata ttagatttaa
    10921 gcttagtttc attatcacac taacttcaaa tttactctat aatccttaaa tcaagcacta
    10981 atttattaat caaacactaa tctattaatt tatatttctt tagacatcta atatagtttg
    11041 ctactaaata ataattattt atatcatatt tatctaatcc tttaaaagaa gttatttcat
    11101 cattaagctc atcactacct tctacaaaaa cacaacctgt ttcaaattcc atacctacaa
    11161 taatcttaca gccactataa gtttcctctt gagtatcgta caattttttt gcatttcctt
    11221 catttggaat tgtaaataca ataggtttta attctttatc actggaaatc ttttgtgaaa
    11281 aaggcttgtt taacttaata ttattgataa ctacatgttt aaattcctct gaagtttctt
    11341 ctactattac atattttcgc tttaaaaact ctattatttc taaaacagac ttagaattta
    11401 cttttaaaat acttttatat ttttcatata aagatttcca ctgttcaatt ttttcctgtg
    11461 gtacttcttg gatatgtgta cttatttttt cttgagatat ttttttcaat taaactctcc
    11521 tttcaatact taaatatcct tataaatctc acatgactaa agttacaagg ttcctactta
    11581 atagaatttt gcatacttaa atatgtctta ttaattctct acaggatatc tccgtagttc
    11641 ctacgatttt tacataagtt acctatttta aattgttatt agttagatta taaaaattat
    11701 ttattttatc actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc
    11761 ttttatttat aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa
    11821 aggaggtttt ccttatgatt ttaaatagaa ctgagctttt ccgaggatat tctttaaata
    11881 gatttaacga gatatgcaat atattatcag taaataaaat taaatataag tataaagtga
    11941 aaaataatgc ttactctaaa aaaccttttt ttaatgaaat aacacttggc aatttaggtc
    12001 aaaaggaaga tttctcttat gagtacttaa tttatgttca taaagacgat tatgaacagg
    12061 cagagtctct aataaattct aagttgcgat agattattta taaaacatga cattctgttg
    12121 tcatgtttta tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct
    12181 ttaaaactag ctttttatag taatttaaag cttaaaacta aggagactcg ttatatgaaa
    12241 ataaatataa aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacagc
    12301 agaataatat cctaccttcc taatataaca atagaaacag acaaaataaa aactataatg
    12361 attaatgagg ttgtcccaac taacaccaat gacgattttt atagtgtaga taaagatgct
    12421 gattatttaa aaactactat tcctttattt gacagtgcag gaatagaagt atctaacata
    12481 aatgatatcc tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa
    12541 tatacaataa ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa
    12601 ttatttaaaa acctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat
    12661 atgattacta aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt
    12721 cgtaataatg aactttatta tgataattta agagtgtttc catcatatat tatgactggt
    12781 ggaaacatac tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg
    12841 ttcgagatta taaacggttg atattacaaa ataataaaaa ctaatttagc caaataataa
    12901 gtgcctcaaa aacttctatt tatgaggcac ttttatttta tattagtgta actatatact
    12961 attaacataa tttattattt atattgttct ttttaatatc tattctattt agtttaggta
    13021 acattactct ttacaagatt actttatatt tcttttatat ctctaacatt tttatttaat
    13081 aaaacaatta ttaatacaag ttaattatat ctatttacct aaaaatatat cttttacagt
    13141 aatatctgga tggtgatact tcttcctatt cgttaaaaaa gattttttta cattaatagc
    13201 ctcttttgga caccagtgaa aacaagctag gcatctttca cactcattac cccactttgg
    13261 ctttttatca actatactta tattctctac aggacaaacc cttttacatg tcccacatcc
    13321 attacattta ttttctgcaa aaaatttagt agttgcaatt tgcatattct tacctactaa
    13381 acgaagtaca acactatcta tatgtgactt taaattgttt tttccttcta tatttccaat
    13441 ctccaagtta ttgatacatt ttgcaatctc agctacccta ttagtctgat ttttcaatct
    13501 ttcttcttca acatctgaag gtgttatcaa agcatttcca ggcatattga ttaaaaaacc
    13561 agcagataat tttttccccc tttttttaag taacttatct atagtaaata aactatgtcc
    13621 agctacccca ttacaagtgg caatggcata tatatgagta ttaatattaa aatttaattt
    13681 ctctacaaaa gactttacta tatcaggaac atcagcaaaa taaacaggaa aaacaattac
    13741 caatatatct tcatttatgt ctatattatt ttcttttcta tgtatagcta agggtataac
    13801 ttctttactt ccatttaacg tttcttttaa aatatttgaa actactagcg aatttcctgt
    13861 tccagaaaaa taataaatct tactattcat tcttatccct ctttctatat ttctacatca
    13921 tcaatagtta aaaagtctct taaaatagga tttatattta ctttccaatt ctatccaaaa
    13981 tatcagtaaa caaaggcaat tcttcatcag agatagattt aaatgtctct gtcgcatata
    14041 tacttgcaat ttcatttaat ggttcttcta attctctacc taaatctgtt aggaatattt
    14101 gaaacttacg tctatcttta acatctactt tacggacaat taatccctta tttttcataa
    14161 catctaagat tcttgtagta ttagatttat ccttacatga tttttcacat aaatctatct
    14221 gagaaattcc atcttgctcc catagaaacc ttaataacgc ataatggtca gttgttatat
    14281 catatccatt ttgtttaaac atctttttta aatcatcttt tacttttaac gaagtactta
    14341 ctaacttagt tgtagcttca tatagtttct caaaatcttt cattttacct ccatttaaat
    14401 aatcccttgc tcctcccatc tatcaattat tcccttatat tgctcaatat ctttaaaagc
    14461 cattggcttt aaaacagcat ttatagcaat aaaattatag atttgagaaa gcacaggtgg
    14521 atttttcttt tcctctatgc aataaataaa ttttttagat accttttgta tctttgattc
    14581 tactttttca gttataggat tttgattaaa tccagttttt atagataaac taccacaaac
    14641 ataacctccc gaatctaaga ccatggattt tataaaagaa attgcttttg aaccacgtgc
    14701 attttcatat gtagtaactg ctatacatgg cttacgatac aataatcttt caagcatcat
    14761 atgtgctcta tctgtaaaca ccttgaaaag tccagttaca ctacttccat aagttgggct
    14821 accaaatata acaccatcac tcttagtaat tatatcatga atatactcta ctttatcatt
    14881 ttgattgata cacttgccca ttttataaca atttaaacaa ccaatacaat atgatggatt
    14941 tacctcactt aaatcaaaat aattaatttt tacatctggt attaatcttt ctatatcttt
    15001 atacaagtaa gttaaaactt ttgatgtagc tccatttttt ctagggcttc catttataac
    15061 tgtaattatc atttctatat ctccttttta gttgttatat caacaataag tatatcaact
    15121 atatttttag ctgtcaatat aaaccattta tttaaagcta tacttacaca gcttaaataa
    15181 aagttttatt taactacatg tgcttattta atcaactata aatgaattat ttttaataat
    15241 tgacaaacta aattaatgat aatatacttt atgtattaca aatgtaagta gaaggaggta
    15301 tagtatgaaa aagttaccac atatttcaga agcagaatat caagttatga aaattatttg
    15361 gaaatatgct cctatcagca ctactgaagt aattgaaaag ttggtagaaa caagtacatg
    15421 gagtcctaaa acaattcaaa caatgttatt gagattagtt aaaaaaggag ctttaacata
    15481 tgaaaaaaat agcagagtat tcgtttatac tccacttgtt aaagaagaag aatacgtagc
    15541 tacagaaagt agttcttttt taaatcgttt ttataatggt actcttaatt ctatggtact
    15601 aaacttttta gaaaatgata aactatctga agatgatatt gaggaactga gagaaatctt
    15661 aaataaaagg acgactaagg gggataaata aatatgtatg ccccattctt tatacgattt
    15721 ttactcagta cattaacctt atcaatatta attgttgtaa ttttacttgc caaaaaagta
    15781 tttaaaaaac atatttctgc aaagtaccaa tacaatattg gatttatatt acttataatg
    15841 ttaataattc catttgtccc tcttaaatat attaatcttg gaaatgtatt taactattta
    15901 tatactttta atcaacttgg aagcagtact aataggactt ctatgataaa aaatagtcca
    15961 aattctgtaa ttaataactc aaatgcatta aacgattttt cagtatccct aaaccaagtt
    16021 aattttgata gtttaaatat tttattacta ctatcatgga taattggaat tttaatcatg
    16081 atagctatta ctattcattg caacattaaa ataaaaaata tgaaaaaatc aatacaaatt
    16141 ttaagagatg atgagatagt atttatattt gaaaagtgta aaaaagatat tggtataaat
    16201 aaggatttta tactatgcaa atcacctatt ataaatacac ctattacatt tggatacttt
    16261 aaaccacaca ttatattacc agataagtct atatctaaat tatctttaaa agatataaaa
    16321 tatatcttac ttcatgaatt acaacatttt aaaaataaag atatactgat aaattatata
    16381 atgtgctttc ttcaaatatt atattggttt aatcctcttg tatggtatgc atttaaagaa
    16441 atgcgtatag accgtgaaat tgcatgtgat atttcagttt taaaaaagct taataaagat
    16501 agtcatatag aatatggaca aactctaata aactttgtgg ataatatttc aagaccatca
    16561 aatcttacac ttacatcaga tattggtggg tcaaaaaaac aaataaaaaa gcgactattg
    16621 aagataatgg attttcataa tgaatcaaat acgttaaaat tcaaaagcat tttaattttt
    16681 acattgatag ctttaattgt atttggaaat attcccaaac tatctattat ggcagatatc
    16741 aacgaaaaat atagacctac aaatgaacaa actgtgtacg aggacttaag tcagtatttt
    16801 aaaggttttg atggaagttt tgttatgtat gatttaaaat ccaagcaata tcatatttac
    16861 aataaagaaa aaagtaaaac aagaatttct ccaaattcta cttataagat ttatagtgcc
    16921 ttattgggat tggaaaatgg gataataaca agaaataatt cacatatccc ttgggatgga
    16981 aagcatcatt ctaatagttc ttggaatgaa gaccaagact tattttctgc tatggaaaac
    17041 tccgtaaact ggtactttca gactctagat aaaaaaacag ggctaaaaaa catacaaact
    17101 tatcttaggc aaattggata tggcaattat gatgtttcag gtggtatctc aaacttttgg
    17161 ctagaatctt cattaaaaat atcacctatt gaacaggtag aattacttga aagtttttat
    17221 acaaataagt ttaaatttga agataaaaat atagaaacag taaaagattc attgttaata
    17281 tcaaaaaagg atggggtatc actttttggt aaaacaggca ctgccaatat aaatggaaaa
    17341 gatgtaaatg gttggtttat aggttatgtg gaaaaaaatg aaaatactta tttctttgct
    17401 acaaatattc aaagcaaaga ttattctaat ggtagttcag catctaagat aacattatcc
    17461 atattaaatg acaaaaaaat ctataatcct taatatattt actgtacatt ctaaaaaatt
    17521 cacataattt tcatattaat tatcaattaa attcatatac aaggcttatt atataaacat
    17581 agaagatagt aaaactagct taaaaacata atataatacc ctatacaaat agtggcaact
    17641 ctccctcaca tcctcaattg ttgccactat ttaatttttt gtatgaagtt aaatatattt
    17701 tcatcatttc aacaatttgg gtgcaataca atctctattt tgaaacaacc tctttaattg
    17761 ttttagagtt tatgaattat ttctcatact ttaaaaagat tgaattactc ttgataatct
    17821 tcttttgtag tcattacata tcacctacta ctcaacacat attctttgac ctatttttac
    17881 atccttgttt atatccaaaa tatttacctt cttaccatct ggaaacaata ccattgtcaa
    17941 agtagacttt cctaattcat gtattctctt taaatcaaca ttaacaagaa catctccctg
    18001 tttaaccata tctccttgtt taacagcaac atcaaagcca tctccattta gtgcaactgt
    18061 atcaattcca acatgaatta atacttctcc attagttcct ttaatcccaa atgcatgtcc
    18121 acttgaaaat actgtttcta atttacctga aataggagaa acaatttttc catctataat
    18181 atcaatagca aaaccatcac ctaacatttt tttagaaaat gtttcatctg gtacattttc
    18241 taatgcaaca attttcccct tagctggaga cacaaagttt tcattatttt cattaaacac
    18301 taattcttcg tcttcattta tagcttcttt aggcactcca aataaacatg ttacaataaa
    18361 gcagaatgca accacaaata ttgatactaa taagtatgtg attaaattat atccatcata
    18421 gatatacatt aaataagaag gtaaaactgc taatccatat gaattagatt gaatatgtaa
    18481 aattgataat attgctgctc ctaaacctga agatgcacac ataataatca aaggctttaa
    18541 attatatcga agctgaatcc caaataaaac aggctcactt ataccaaaaa gttgagatag
    18601 cattgctcca actgcagttt gtttaacttg attattttta gattttttca taaatgctag
    18661 acatgttcca atattcgcaa atccatacat tgcacaaaga gtaataaggg cattaaatcc
    18721 tgtatttgct aatagtgatg tttcaatcat tacataagta tgatgtaagc cagtaataac
    18781 agctaatgga tatgtaaatc caataataaa tcctccaatt cctaaaggca aatcaatcaa
    18841 taagctgatg atatcaacca tttttaattc tattgtatgc attatagggc ctacacctaa
    18901 caaaataact aacattgtta ttagcataac aaaaaatggt gtgaaaataa ggtctaatac
    18961 attaggcata tgtttacgga accatttttc aagattagct ccaataaatg ctgtaacaat
    19021 agctgttaaa acacttcctt gacaacctac aataggaata aatccaaatg ccataattgc
    19081 ttcaatttca tttgatgggt cagcaacaga atatgcatta ggaagtattg gggaaactaa
    19141 cataagtcca ataacaagtc caataacagg tgttcctcca aatactttaa atgctgacca
    19201 tacaataatt gctggtaaaa aagcaaatgc tgtttctgta agaactgaaa caagtgtctg
    19261 aatatacaat ggaatatttg cagaagacat tccaaataat ccaagtacat tatcattaaa
    19321 caaacaaccc tttaatccca agaataatcc tgttgctgca ataactggaa taataggaac
    19381 aaatatatct gctaaagttc tcataacttt cttaaatcca gtttcattat ttttaactaa
    19441 ttcatcttgt tctttttttg ataaagtttt taatcccatt ttttcaactt ctgcataaac
    19501 tttattaact attcctgttc ctaaaataat ttgatactgt ccagaagtaa agaaaactcc
    19561 tttaacctca tcaacttttt caactttctt atcattaata atttctctat ctttaaccat
    19621 cactcttaat cttgtggcac aatgtgtaat agaaataata ttatcttcac caataatttt
    19681 aataagctca tttgctattt tattatattt attcattttt ctacctcgct ttcaataata
    19741 ctgcatgaaa tggtttcaaa cgcactgttt tatttaatag atttacatca tctaaatttt
    19801 ggaatacaat atcatatttt tcttctactc ttatttctac aggtttatca gaaaaattat
    19861 aataacaatc aataactacc aaatctaatt gccttctata cgcaataaca tcttcacttt
    19921 ctatatcaat aggttctatt gaaccataaa tcaaacaatc agaatactca ctatcttgtc
    19981 tcaactttat catcttttta taaaattcta aaatactctc tgaattgtta atctgactct
    20041 gtgcattaat atctttataa ttttcaataa cctttaacca tggtgttgta tctgaaaaac
    20101 ctgcattttc tttatctgcc catggaaatg gcgttcttgc attatctcgg cttcttaaat
    20161 tcacaaattc taacgcttct tcttctgagt aaccttcatc tattgaacga tagtattggt
    20221 caatgctaga taaatcatta aaatcattta tggtatctct tttaaaattt gtcattccta
    20281 attcctgccc ctgataaata aatggggttc ctcttaaaaa gaaatacatt gctcccaaca
    20341 tttttactgc atcatcattc ctttcgtatt gcaaaagata ctttgttgta gctcttggtt
    20401 ggtcatgatt ttcaataaaa ttagctgccc atccatgttc ctgaacatac atttgactat
    20461 ctaaaatgca ttttcttaaa tctggaattg tccatgaaat tcttttaaac cactcactac
    20521 cagaagcaac atctaagtca gcatgtttaa agtcaaagac cattgaaaag aaaccatctt
    20581 ctccaataaa atcacctaac tggtcatagc taatacctgc tgcttcagca acagtcatac
    20641 aatcatattt tttaaacgta ttttctttta attcataaag cattttttct attccaggct
    20701 gatttctaac tgtttttgta catttaacaa gaccatcaac accatcactt tctaaacttt
    20761 gaaaacttaa attctttttg atgaatgtaa tagcatcaac tctaaatcct gccactccca
    20821 tttgagacca ggtattaatc atttggtaaa gttcacttct taatattgga ttttcccaat
    20881 taaggtcagg ttgtttttta ccaaaagaat gaaaataata ttcgtctcta ttctctacct
    20941 tttcccaaac acttccacca aaaacagacc tccagttatt tggtaaatcg tttccttttt
    21001 taaaaatata ataatcatga taaggacttt ctggattttt taatgcttct tgaaaccaag
    21061 gatgttcatc acttgtatga ttaagaacta aatctaatat aattttgatt cctctatctt
    21121 tactttcttt aattaactct tttaaatctt ccatacttcc aaattcactt gcaatatcca
    21181 aataatcact aatatcatac ccattatcgt ccataggtga tttaaaaatt ggacataacc
    21241 acaatgatgt aatacctaaa ttttttaaat aatctaaacg agaaataatt ccctgtaaat
    21301 ctccaattcc atcattatta ctatcttgaa agcttcttgg ataaatttga taaacgattt
    21361 ctttttgcca ccatttcttt tccataaatt actccttttt atatgatatt tcttcttttt
    21421 aattctttat aaattccatt atcgaatatg tcctcacata tagatgtcgc tatatcttta
    21481 agttgttgat gactattttt cattgcaatt gtaacgtctg atgcttgaaa catgaaaatg
    21541 tcattttgac catcaccaaa acatatggtt tctttttgag ttactcctaa tctttcttgt
    21601 aatctcttaa ttgctttgcc cttatgaaaa tctttttgaa taatttcata atactgacta
    21661 gaaatatctc tctgtgctaa ttccatttta tcttgtagta tatctttcac ttcgtcaaat
    21721 accttttcat ttgaccataa acaaatttta tgtatatctt gtgacttata ctcttcaatg
    21781 ttattttcat atgttatttt ttcttggata tgttgtttat ttatacaaga attagttcct
    21841 ttcaactgat tcattgtttc aaatatttct ttggcttttt gattcatgaa aactttttcc
    21901 tgactctcaa tcgaaaaagc aacttctcta ttttttaata aacaaacaac ttcctttatc
    21961 aatctttgat taaaagattg attataaagt aattcaccat gatattgaat gtaattacct
    22021 cctcctgcaa tatatccatc aactcctaat gataaaacat catcttgtat tgtacccata
    22081 cttcttccag tacaaataac aacactgcaa tgattctttt gacataaacg tatagcatgc
    22141 tttgcagatt ctgggattcc atatacctca tctctaagcg taccatctat atcaagtatt
    22201 aataatttgt acaatttcat cttcctttct tactatcaaa atcataatag cattcaaaaa
    22261 aaaagatatc aatattttga tatcttttaa aaaatatgtt catttttagc acaaattttc
    22321 attttcgggg aatatgttca ctttctaaat acatcttgta tttaatataa agcagttcaa
    22381 ttaaaataaa ataaggtgtt gttacatcgt agtccagttg tttatctaca gaaatagata
    22441 tagattgaat atacaggttt tcatcacata atcttgctaa aggattactt atcatttttg
    22501 taatagaaac catataaatt cctttcattc tcatttcttt agcaaaatca ataattgtag
    22561 atgcatctcc attcaatgaa ataaagaaaa tcatatcatc tgtttttata aaatgattaa
    22621 gtgcctgaat catatcataa ccataaacat catatataat tttccctgtt ggtaagaaaa
    22681 tccttttcat ctctttagca actctttcct gagaataacc ttctccaaag acgattatac
    22741 gatttgcctt tttcattctt tcaaacataa tcgaacaatc cttcttctct atatcatcta
    22801 tcaccttatt ataacaatcc attaattggt gtttactttg atttttatat tctacttctg
    22861 tttcatttaa gcgtataata actcttaatt cactatatcc aggtaattgt agtttttgag
    22921 caaaacgaga aagtgcactt gtagatatat ggtatttgtc tgcaaattca tctatagaaa
    22981 gtttaataca atcttcttta tgattaagaa tacactcaca gatatactta tcatttgctg
    23041 taaatgaatt ataatattta ttgaaaagtc cttcaatttt cattcttttc tccttctata
    23101 ctctatgtaa ttcatatgaa gcaaatctaa tagagcataa aatgctccta cgacttcata
    23161 agatatagct tgtaatgtat caagtttttg cgtttcaaca tacagattat actcacaaag
    23221 tgttgcaatt gtattattat caagcctagt taaagaaatc gtatgtatat gattgttgat
    23281 aaattttaca atttcaattc ctgcttttgt ttctccagac aaagaaatga taataaataa
    23341 atcacttttt tgaaatgatt ctctcataaa ttcaatttca cctatatcaa ataagtcaat
    23401 aacacattta cctactgaaa gaaatattct ctttatttct tgagctaaac ttttctgttc
    23461 attacctgtc ccataaatat aaattgtttc tgcatcatat attattttac aaatatcttg
    23521 aaatgatatt ttttgtaatt cattcattaa tagtcgataa ttctcgcaaa catcatgaaa
    23581 attattatca ttattaacaa attctagttg attattttta agtaaatact ttaaatcaga
    23641 gaatgaatct aatccaattt ttctacaaag ccttaataaa gtagctctag aaacattaca
    23701 ttcatttgca attttttcac acgaaaaatt acggtattct ctttgacaaa tcaatatttg
    23761 tttaataata attttatcat tttcatttaa attatcataa ttttggttaa ctaacgattc
    23821 taatctcatt ttttccactc cctaaatatt attttaatat acattagttg ctttttctag
    23881 ggaagagtct taaaaatatt tctattaaga caaataaaaa agagctatct cagaacaaat
    23941 ttaatttgct cataccacag ttctattata aataaaaaaa ttttcacatt attttttaca
    24001 ctatataatt tctattttta taattaaacc caatttataa tatatagaac catatttata
    24061 tttttatctc ctgatttgtt ttaattacat ctattcattt tctatcttat ttaataattc
    24121 ctattacttg aatatttact tctttgagta aatttcatag ctaacaaagc taaaattatt
    24181 attataaatg tcaaaataca aatataattc atattaaaaa agcttccatc tctttcttct
    24241 atataagtca caaaactaat aaacaattgt gttgggctta taatactaat tttttgtaac
    24301 attgttggca ttttttcaac tgaaataaat gaaccaccaa taagtgataa tataagtgat
    24361 atacatgtag aaaacaaatt tgcatgcatt tctttattga ataaagaaga tacaaatgtt
    24421 ccaaaagaag ttgccaaagc tgatatccat actcccatta aaattaaatc tatataatcc
    24481 ataccaatat caatattaaa taacttaatt atagatattg aaactatgta ctgaaaatac
    24541 gttattgtaa agttgtataa catattacct agcacatatc ctcttatatt ttgtggagaa
    24601 tatagcactc ttgtaaatgt acctgagttt ttatctttaa taatcattga tgctgttaca
    24661 gtagatgtta tcattaaaaa taatacgata aatgcaatta ttctttgtaa aactcctaaa
    24721 gtattttctt gtaatttact tatatctaca ggaactgggt cttttttata tgtttctatt
    24781 aaatacttta atgtatcatt cgtattttta tcttttatag aatataactc aaaaccattc
    24841 ttattaaaac atatgacagc tgaatacttg cctgttataa tatcagtttt aattgtttta
    24901 aaatctgcaa tctctgtatt aatcccttga gtattttcta aagccattat tgtattttca
    24961 taggttcttt tttcagattc ctttagaatg cattcgtttt tgtactcatc taaaatacca
    25021 atattaaaag atggtttact tatgtaattt gccaatactc ctaaaactac tataattata
    25081 ggaaataaaa gtgtaactaa aaacactatt ctcttattta tatttctttt taagttacta
    25141 ttaaaaacat ttattacatt cactatataa agacctcctt tttaaaaaat ttgattgtaa
    25201 gtattgtcat aaatatgctt acagctacaa atatgcatga tatattaaaa aggagttgta
    25261 aattattatc atagatacaa gcaactatgc ctctattaat ccatgttaat ggtgatatat
    25321 taatgattaa tgaaataact ggattcaatg aactaactgg aaagaacact cctcctaaga
    25381 agccaaaaat aactatcggt atattaatga tattacgtaa catatctaaa tttttacaaa
    25441 caagacccaa aaacaacccc atagatacta ctgcaataga ttcacacatt aaaaagaaaa
    25501 taacaactaa aaatttccca ccaaaactca tatgaaaaat tcctcttaac accactaaag
    25561 ttatcaaatt gtatatcgaa aatactgttg tgcaagaaaa aagttttgaa ataactaaat
    25621 ctactattga aataggagaa atcatataac gatatgatgt gttagaaatt ttttcatctt
    25681 gtgctacata tgaaacacta ataattccca taagaacaca aaatggtatt gtaacaatag
    25741 tataataatt atatgatgta aattccttac cataagactt tgatgtcaaa tatcctaata
    25801 gtactatgat tattactgga aaaacaatat tgtaaaacag aataaaaaaa tctttacttc
    25861 ttcgttttat tcctaattta aacaatgtta aaaaacatct catctattta ttcctccaaa
    25921 tctctcaaat ctgtacctgt aagatataga aaaatatctt ctaatgtctt acaaccaaat
    25981 tttaaatatt tatttttata taaactcttg tctaaatctt ctataattac tcctttatct
    26041 attaaaacaa ttctattaca taaagtttca atttcctcca tatagtggct agtatatatg
    26101 actgttgtgc cattattatt aatgccttta attgcttcca aaattttatt tcttgattga
    26161 gggtctatac ctactgttgg ctcatccatg atgagaatct gtggactatg tgcaattgca
    26221 caagctatgt ttagtctacg tttcattcca ccagaaaatt ttgatggaaa ctccttatga
    26281 ctttcccaaa gaccaacaaa ttccaaggca tatttagttc tctctttaag ttcattccct
    26341 ttaaatccat ataatgagca aaaaaattta acattatcat atgcacttaa atcttcatat
    26401 attgctaatt cttgtggtac aactccaagt gagcttttaa aaatattttg ttgattctta
    26461 atatcttttc ctttatagta tatgcttcca ctatctggag acacaatcgt agtaagcata
    26521 ttgatagatg tgcttttccc tgctccattt ggtccaataa aacctaaaat atctccttcc
    26581 aataacttaa aactttctct tgatacaact tccttattgt tatacttctt agataaattt
    26641 ttaatctcta acaatacatt actcatttat aaaatcctcc taatatttaa ccaacattat
    26701 atatccaata ttgtatatat agttttttta aaacacgagt gttttactcg tgttattata
    26761 ataaaattta ttacttattt tgtaatttca gtattatctt atctacattt tctatagctt
    26821 tatctggatt attaattatt tcatctctaa gtctttgaac ttctaataac tcatttgtcg
    26881 tatcagatgt aaataaaaaa tcttcttcta tatttgaaaa gtctatagat ttaattatat
    26941 tctgtgtctc gcaaccaaca gatatatact tatcgatatt atttaatatt tcttcatgcc
    27001 acaaaatttg ataattcaga ttatctatag tcatatcaaa tgcaattttt tctgttgcaa
    27061 gactcttttt atcaattttt ttaatctctt tccaattttc ccaatacttc ttctgctcta
    27121 taagatattt tatatgtttt tcaagatttt tttgcaaagt atccttaggt aatacatcca
    27181 aaatattatg taataggaat ttatttgaac cagttggaac tattggcatc tgaagctctt
    27241 tttgtatttc ttcccttaat tctaatttac cgctttgagt aatctcatag attttacgaa
    27301 tcctagctcc tgttttttct tcttttaaaa ctctaactcc tccatccttt tctaattttg
    27361 ccaatgcata atatattgac ccagattgta ttttagtcca tctatctgca ccagatactt
    27421 gtaaaaactt ttgaatctca tacccatgtg ttggcttaat atttagataa tataaaatta
    27481 aagtgcgtat cataaaaatt caccacctat actctatctt ggatattata ttatcaccaa
    27541 cattggatat agtcaataga taacatagga tatattacaa cttattattt ttattattta
    27601 atacaaggag ttccatgaga catacatatc cattcatatt ttaaattatc caattctctt
    27661 atagatttca tcatcttttt ataatcccaa ttccatggat taggataagg aactatattt
    27721 ccattcttat ttttgactaa atcacctgca aataatacat cttcaaaaat catgcagaca
    27781 tgccctggag tatgaccagg agtatggata atttttatat tcccaatctg catattctct
    27841 ccataaggtt caacatcctt aatgagcctt ctggaaattg cctttccaat aaacttctta
    27901 aacccaggtc tatccttctc tcctataata aaaggtatct cctctacatg agcccatact
    27961 tttgctcctg ttttttcttt caataattta atattagata tatggtcaac atcatgatga
    28021 gttaataaaa tatgcttaat atctgtaagt ttaataccaa gtgtttttaa ttctttaatt
    28081 atattttccc ccataaaagg aagacctgtg tctataagaa taatttcttt atcaaacaca
    28141 ataaaacaat ttgagccacg agtactatct aacataaata cattttcttt tatcttcata
    28201 aataaattcc ccttatttgg tattttacct atataattaa ttaataaagt tataaatagt
    28261 tataatactt gatattaatt ttatttttta ttaaagtata ctataggtat acaaatatta
    28321 tatttacata aatataccat cagtatactt ttttatcaat agaaaggaat aatttatatg
    28381 tcagaattat caaaaagaga taaagaaaaa atacaaagag aaaatgagat tattgataag
    28441 gcagaaaaat tattttgtct taatggattt gataatacaa ctatgaatga attagcaaaa
    28501 gaagttgaat atactaaacg caccatatac aagtactttt catgtaaaga agatttattc
    28561 tttgctgttg tcttaagagg atataaaaga ctttgggata atgttaaaat agaaagtgca
    28621 aaaggcaaaa cagggtttga aaaaataaaa ctttcatact ttgcttttca taaattttac
    28681 tctgtagaac cttctctatt atgccttatg ggaatgattg gtattgtaaa ctctgaaaaa
    28741 tcggatactg atatgctgtt taaagaaaag tttttttctt ttaacaaatt tatgtttgat
    28801 gaaatacaag gaatgtttga aattggaaaa aatgatagaa gtattaggca tgatattgaa
    28861 atacctactc ttatgtattc atcaatattt acacttacag gattttttaa tttactatct
    28921 gtaacaggaa aatcatacct gaacaacttt aatatagatg aggaaaaatt tattgaaaca
    28981 actcttgctc ttttaataga ttcaattaaa gcataaacct aagtttataa attaaagttt
    29041 atatacttaa atgataattt tttctatctc taatataaaa aatattaaac ataaaactaa
    29101 taacttagtt aatggaataa ctaaattact gaaaggatta aaagtaaatg gctaaaatac
    29161 ttcttttaga agatgatgat acaattacat ttggaataaa aacttccctt gaaaagaaaa
    29221 atcacactgt atgttgttat aatacgattg ctgaagctaa aaaaaatttt gataaaacta
    29281 ttgatttagt tctgcttgat ttaaatctac ctgatggtat tggatacgag ttttgccaat
    29341 atgttaaaag tatagaagat actccagtaa tatttttgac agtccgtgat gatgaaaaag
    29401 acattatacg tggattagat atgggtgcag atgactatgt ggttaaaccg tttttgctct
    29461 caatacttca atccagaatt aatgcagttc ttagaagaac ttattcaaat actaacaata
    29521 ctattacatg tgaaaatata agcttgaata aggttgaagt caaagcatat ttagataatg
    29581 ttgaagtacc tttgacagca ggagaatata aacttcttct aagtcttatg gaaaataaaa
    29641 atagagctat gactcgtaca aagttactag aaatgctctg ggatgtagat ggtaatttcg
    29701 taaatgataa cactcttact gttacaataa aacgtcttag agaaaaatta aaaaatccac
    29761 cttgtataaa aacattaaga ggcataggat atagaatgga ggacaactag tgaaaactcc
    29821 aagaaatatc aacagtacag ttgtttttat aatattctgc ttaattgtaa cttttttatt
    29881 gactgtaatt ctaacaacta taacttcttt taatgaatac aatgtactgt ctaaggctct
    29941 tggtgcatca tatgagtaca atcctaagtc tacagaaagt atactaaaat cactgaaaaa
    30001 ttctagtgat aaggatttta aaacaggaaa tgaaatttta tcaaaacatg gatattctaa
    30061 aagtacattt tggagtaaaa acttttttta ttttctagtg atatcgtttg gtataatttc
    30121 aacaatggct acagttgtat atataatcaa acatagaaca aataagcaaa aactgagtag
    30181 aataaacaat cttactcaat acttagaaga agtgaattta ggaaaagagt ctatactttt
    30241 acgatgtgaa gatgaatttt ctcaccttga agatgagata tataaaacag ttggggaact
    30301 tcgttgttca aaagaatttg ctttaaaaga aagacaaact ctttcagata accttgcaga
    30361 tattgctcat caacttaaga ctccaattac ttctatgtca cttatggcac aattactttc
    30421 agaaaattgt ccagatgaag aaatagatta tattaacaga cttacaaatc aaatctcaag
    30481 acttgagaga ctagtttcat ctttacttac attatcaaaa ttggatgcaa gtactttaat
    30541 atttgaaagc aaacttatag atgtacattc tcttcttact tatgcaattg agcctataga
    30601 aggttcttta agagaaaaac atcagacatt tactatcaag gcttctcctt cggtagaatt
    30661 tgaaatcgat attaattgga ctttagaagc tcttttaaat attcttaaaa attgtagtga
    30721 gcatatagaa gatggtggat atatagttgc ttattatagt caaaatcctc tatatgttga
    30781 gattatcata gaagataatg gtaaggggtt tgctcaagaa gaacttccat atatttttaa
    30841 tagattttat cgtggtaaaa atgctagtaa ggacagtatt ggtatagggc ttgctctatc
    30901 aaagtctatt atagaacgtc aaaatggcac tctacatgca gaaaattgcc ataatggtgg
    30961 tgcaagattc ataattaaat tttacacata aaatgctgga ttataggtaa aatacctata
    31021 atccagctat atctatattc tatgttatct attgttttag taatatactt ctatttacat
    31081 ttaaggtttt attaaacaaa caacttaact atccctatac ttgtaagtag tcttaaatcg
    31141 aaacctttaa ataagttcaa atagcttttg tttatattgt ttcatttttt atagattcta
    31201 ctatattttc attcttcaac tgcttgtatc ctgtcatata tgatgctatt acaataaaaa
    31261 gtatcaatat agtaaaacct attattgtag atataggtgc aaatggtaaa tactctataa
    31321 aatatatttc atttatacgt agaaaagcat acactattcc tatttgcaca ggaatactaa
    31381 gtagtaatgg tgtaattcct aagaacaaac cttccaaaac aagcatcttt tttatgccat
    31441 ctggagataa tccaacagaa cgcaacatag caaactcttg tcgtctactt ctaatatttc
    31501 ctgacactgt agcaagtaca tttgataatc caatcatagc caataaacct gtaataaaac
    31561 ctactatcaa attcatggtc tttcttccac tagcatcttg tttttcactt tctaaaacat
    31621 cctcaatact atagtctccc gaaccatagt ctttattaca aattgactct aatttttctc
    31681 ttactctagg aatgtcctca cgattattta ctttaaatag tcctataagc ctatttgaac
    31741 gtaacctacc tacttcatca taatttttag atatttcctg caccatagac attggcacta
    31801 tttgcattaa tgtatatttt gaactagtat ctactatttt aggaagtgtg cttgtaatag
    31861 ctcccacttt catgttgtaa gtataatccc ctttatcttc gtcatatact ttttcattta
    31921 atttaatgtt atctcctatt gaaagattta aataatctat atatacttta tttctacgtg
    31981 tactacgatt aatatcttcc tccttattat atacaattga ggttggattt tttgtatcat
    32041 aaaataactt tgggtctaca gaaatttgcc tacaatattc tgtaaaactc ttatcatcaa
    32101 gcccaattat atttgaaatt atacgaaact tgccatcacg ttctatagga gagtactttt
    32161 tagatgaagc tatattttta aatcctccat ttttcgcaag ttcttttgaa gcatcttttt
    32221 cagtcaacca agtggaagca gacatttttt gataatatag tgaacttttt acaccttctg
    32281 tattatctaa tttttcaatg aaagcagact ctacaggttc actattttcc aaataaactt
    32341 ctatatctct ttggtcctta taattatcac ttccataaac aacttttgct ccttcttgat
    32401 tggcaattat atgcaaaaaa cctgtcagta ataaaaaaga taatgttaaa gatattgttg
    32461 cagtacgata agatttttta cgagcacgta gtgcatttga tgcaagttct ccttctattc
    32521 caaatatctt tcctaatgaa tatttatgcg actttttgat tttaacacta tctccttgtc
    32581 gtattgcctc tataggagat atctttgata cttttcgtgc tggtataagt gcagaaatcc
    32641 agactgttac tattgtaagt aaaactgctg gcagaaaagc aggaatacca taagtaaata
    32701 ccacttctgg agcatcagca tgataattac ctgaattaat atagactata agcctattac
    32761 acaataacca tcctaaaaat aaacctattg gtaatggtat aattgtcagt aaaaatcctt
    32821 caaacactac agacctttta atttgttttg gtgatgcacc aacactagat aatatcccta
    32881 actgagtaag tctagcattt gcagacagtg aaaacgcgtt gtaaatcacc attacaaaca
    32941 cggctactgt aaaaattgct attgtaagat acataatagg agttgaaaat tttgatatac
    33001 tactcataga tgctttctgt tctggtgaaa agactaacat ctttaaaaga taattactat
    33061 tgtatttaag attgtatttt ccatattcat tagtcttcca ccccaatgat ttagcaagtt
    33121 ttggaagttc cttatacgtg tctcttatat ttttaaatcg caagtacaca gttatactat
    33181 cattaggttt tatacttgtt ttatcaagat atccaagagc tgtatatgca ggtattactg
    33241 atgaagtaga aacatctacc tttcctacta ttgtcaatgt agcctctgaa atttttttaa
    33301 aactttcccc tttcgatttt ggggctatgg gttcaagagg ctttccattt aggattctat
    33361 ctcctattgg gagagtaatt ttatcaccaa tttttacact tggattattc tcaaaatatt
    33421 gttttgataa tgcaatttca ttagcattat ttggtcgacg accctctatt atagtatcct
    33481 tttctggcat agaattccaa tactctgatg tagcatttct ataaattata taatctcttt
    33541 tctcatcatt aattttgcct acataccatt gtccttttac cattacatca tcaactgaag
    33601 aaaaattttc aattaatggt aagtcacttc cataagtata gtcaaacaat tcaccatgcc
    33661 agttaccttc tttctcaatt gaaagtctta taaaatctgt ccacatggta tacaacagtc
    33721 cacacaaaga agacatcatg gttgtagtaa gtaatatagc taacattata gctatactac
    33781 tacgtttatt gcgtcgtaca aagtctaagg tgtattcttg aagaatattc atttcttcac
    33841 caccttttca gagacaatca ctccatcttc catagttaca atacgatttg cctctagtgc
    33901 tattttctca tcatgagtaa taagaagtat tgtctgatta aatcgtttat ttgataagtt
    33961 taacaagtct acaatttctt ctgaattttt tctatcaaga ttaccagttg gctcatctgc
    34021 taataaaata gctggtctat agattaaact tctagctata gatacccttt gttgttgtcc
    34081 acctgaaagt tgacttggaa gatggtttaa tctatcagat aatcccaata tagatacaat
    34141 ctcagagaat ctatcctcat ccacctttct tttatcaagt aacatgggta ataatatatt
    34201 ttttctaaca tcaagggtag gtataagatt ataaaattga taaataagac ctacttttct
    34261 acgtctaaaa actgcaagag cttcttcctt taaacttgat atttctgtat cttcaacata
    34321 aatctttcca gatgtaggtc tatctactcc cccaagcaga tgtagcaatg tagatttgcc
    34381 agaaccagat gctcctacaa tggatacaaa atcacctttt tgaactgata aatttacatt
    34441 attaagggct gtcactctgg tttgatttga accatatatt tttgtgagat tttcacattt
    34501 taatatttcc atatacaatc atctccttta tggttaaatt ttagcaacct aaaatgacat
    34561 tatagtgaca tgaatacaaa tataaattac tattaaaaat attgtttagt ttttgcacat
    34621 tatttaaaat tataatataa aacaaaaac
//
LOCUS       DAEMHZ010000031        34518 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000031 DAEMHZ010000000
VERSION     DAEMHZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.031"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..1120
                     /gene="ytvI"
                     /locus_tag="KPK72_001355"
     CDS             68..1120
                     /gene="ytvI"
                     /locus_tag="KPK72_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2488587.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1210..2436
                     /gene="pepT"
                     /locus_tag="KPK72_001356"
     CDS             1210..2436
                     /gene="pepT"
                     /locus_tag="KPK72_001356"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG2488588.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2643..3695
                     /locus_tag="KPK72_001357"
     CDS             2643..3695
                     /locus_tag="KPK72_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG2488589.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVIEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(4031..4600)
                     /gene="yfcE"
                     /locus_tag="KPK72_001358"
     CDS             complement(4031..4600)
                     /gene="yfcE"
                     /locus_tag="KPK72_001358"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG2488590.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(4625..5425)
                     /locus_tag="KPK72_001359"
     CDS             complement(4625..5425)
                     /locus_tag="KPK72_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG2488591.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            5560..6420
                     /locus_tag="KPK72_001360"
     CDS             5560..6420
                     /locus_tag="KPK72_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488592.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            6420..7106
                     /locus_tag="KPK72_001361"
     CDS             6420..7106
                     /locus_tag="KPK72_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2488593.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            7158..7700
                     /locus_tag="KPK72_001362"
     CDS             7158..7700
                     /locus_tag="KPK72_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488594.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            7779..8246
                     /locus_tag="KPK72_001363"
     CDS             7779..8246
                     /locus_tag="KPK72_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG2488595.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            8955..10091
                     /locus_tag="KPK72_001364"
     CDS             8955..10091
                     /locus_tag="KPK72_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG2488596.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            10150..10932
                     /locus_tag="KPK72_001365"
     CDS             10150..10932
                     /locus_tag="KPK72_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2488597.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            10951..11961
                     /locus_tag="KPK72_001366"
     CDS             10951..11961
                     /locus_tag="KPK72_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2488598.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            11980..12777
                     /locus_tag="KPK72_001367"
     CDS             11980..12777
                     /locus_tag="KPK72_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2488599.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTILVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            12875..13720
                     /locus_tag="KPK72_001368"
     CDS             12875..13720
                     /locus_tag="KPK72_001368"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG2488600.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            13807..14982
                     /locus_tag="KPK72_001369"
     CDS             13807..14982
                     /locus_tag="KPK72_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2488601.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            15177..16079
                     /locus_tag="KPK72_001370"
     CDS             15177..16079
                     /locus_tag="KPK72_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2488602.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIYEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPIEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            16093..17097
                     /locus_tag="KPK72_001371"
     CDS             16093..17097
                     /locus_tag="KPK72_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2488603.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(17241..17468)
                     /gene="acpP"
                     /locus_tag="KPK72_001372"
     CDS             complement(17241..17468)
                     /gene="acpP"
                     /locus_tag="KPK72_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2488604.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            17640..18065
                     /locus_tag="KPK72_001373"
     CDS             17640..18065
                     /locus_tag="KPK72_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488605.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            18113..18319
                     /locus_tag="KPK72_001374"
     CDS             18113..18319
                     /locus_tag="KPK72_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488606.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            18501..18680
                     /locus_tag="KPK72_001375"
     CDS             18501..18680
                     /locus_tag="KPK72_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488607.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            18714..18914
                     /locus_tag="KPK72_001376"
     CDS             18714..18914
                     /locus_tag="KPK72_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488608.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            19167..20183
                     /gene="ccpA"
                     /locus_tag="KPK72_001377"
     CDS             19167..20183
                     /gene="ccpA"
                     /locus_tag="KPK72_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG2488609.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(20306..20704)
                     /locus_tag="KPK72_001378"
     CDS             complement(20306..20704)
                     /locus_tag="KPK72_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488610.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            20916..21821
                     /locus_tag="KPK72_001379"
     CDS             20916..21821
                     /locus_tag="KPK72_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488611.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            21936..22116
                     /gene="ssrS"
                     /locus_tag="KPK72_001380"
     ncRNA           21936..22116
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPK72_001380"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            22299..23516
                     /gene="cdeC"
                     /locus_tag="KPK72_001381"
     CDS             22299..23516
                     /gene="cdeC"
                     /locus_tag="KPK72_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG2488612.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCQMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(23617..24939)
                     /locus_tag="KPK72_001382"
     CDS             complement(23617..24939)
                     /locus_tag="KPK72_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2488613.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(24990..25784)
                     /locus_tag="KPK72_001383"
     CDS             complement(24990..25784)
                     /locus_tag="KPK72_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2488614.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLIDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            25894..27339
                     /locus_tag="KPK72_001384"
     CDS             25894..27339
                     /locus_tag="KPK72_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2488615.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGIFIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            27368..27577
                     /locus_tag="KPK72_001385"
     CDS             27368..27577
                     /locus_tag="KPK72_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG2488616.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            27597..28991
                     /locus_tag="KPK72_001386"
     CDS             27597..28991
                     /locus_tag="KPK72_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2488617.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            29231..29983
                     /locus_tag="KPK72_001387"
     CDS             29231..29983
                     /locus_tag="KPK72_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2488618.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            30009..30422
                     /locus_tag="KPK72_001388"
     CDS             30009..30422
                     /locus_tag="KPK72_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2488619.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            30426..31169
                     /locus_tag="KPK72_001389"
     CDS             30426..31169
                     /locus_tag="KPK72_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG2488620.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            31169..31639
                     /locus_tag="KPK72_001390"
     CDS             31169..31639
                     /locus_tag="KPK72_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2488621.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            31656..32414
                     /locus_tag="KPK72_001391"
     CDS             31656..32414
                     /locus_tag="KPK72_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2488622.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            32398..33240
                     /locus_tag="KPK72_001392"
     CDS             32398..33240
                     /locus_tag="KPK72_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2488623.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(33400..34305)
                     /locus_tag="KPK72_001393"
     CDS             complement(33400..34305)
                     /locus_tag="KPK72_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2488624.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        13443 a         3801 c         6152 g        11122 t
ORIGIN      
        1 attcaaataa aactagtatg aaatggacaa aataaaaata taaattagaa gaaaggagat
       61 ttttaagatg agcatactag aaaataattt tttatacaaa ttaaaaaaca acactatctt
      121 tttttgtata tatacattgt tatttatttt tatttacaaa gcgtttccat acatagctcc
      181 attttttcta ggtgctataa tagcacttat gattaatcct atatcacaaa agttagaaaa
      241 taagtttcgt ataaataaag gcatctctac tcttgttcta agttttcttg cagtggctat
      301 agtcagtaca gttactacaa taatagtaat aaattcaatg aaagaattaa tgggattttt
      361 aaataatata tctgctaatc ctgaggatat aagtaatact attatgtatt ttttaaataa
      421 aataaatgac tttatgaaat catttcaaga aatagccaat tttgatgtag aacaattagt
      481 aaacaaattc agtggtgaag taatgcaaat tacaaaaaat cttcttacta gtatacttgg
      541 attggctact tctattccat atattataat atttataatt accttgttta ttgctacgta
      601 ctttatagca aaagaccttg ataagattga aaatagtttt tataatatgt ttactgttga
      661 tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg ctttcacttg ttggatatat
      721 aagagcctat acaatactta tggctataac attttttgca atctggggaa gttttgcttt
      781 atttggattg aaatatggtc taatagtagg gtttgtagga gctttaatgg atttaattcc
      841 attcctaggt ataataacaa tatatcttcc agtaattgtt tattattttc ttataaaaaa
      901 ttattttata gctataagta tgactgtgat attttttgtt ttgtctttga ttagagaaat
      961 acttgaacct aaactagtat cagtaaatgt tggactaaat ccattagcta ccctagcagc
     1021 gatttttatt ggaatccaag taaaaggaat aattggagta attttttgtt tgggtttggt
     1081 atgtatgcat gatatactta agaaggtgga tattttttaa ttataataat tgatagattt
     1141 tttaaaaata aaattttatt cacaggtaaa taaaaaaata ttaatcttat taagaaagag
     1201 ggtatatacg tgaaacaaaa agtagtggaa agatttttaa aatatgtatc atttgataca
     1261 acatctaatt ctcagtgtga gaactgtcca tcttcagaag gtcaaagagt tttagctaag
     1321 tatatagttg aagagctaaa aacaatgggt gttgatgatg tgagtttaga tgaaaatagt
     1381 tatataatgg ctactttaaa aggaaatact gatggtgtgg atactatagg attcatatca
     1441 catttagata caattgaaga tgttagtgga aaagatatta aaccaagaat aatagaaaat
     1501 tatgatggaa aagacatagt tttaaatgaa gcgttaaatg ttattacgta tgtaaaggat
     1561 tctccagagt tggaagaatt taaaggtgac gatttaatag taactgatgg aacaactcta
     1621 cttggctctg atgataaagc tggtatagct gagatagtga ctgcaataga gtatctaata
     1681 aatcatccag aaattaagca tggagatata aaaataggat ttacaccaga tgaagaaatt
     1741 ggtagaggtg ctgatttatt tgatgttgaa aaatttggag caaaatatgc atatacttta
     1801 gatggaggaa tagttggaga acttgaatgt gaaaatttta atgctgcaaa tgcaactatt
     1861 actatacatg gtagaaatgt tcatccaggt tcagctaaaa ataaaatggt aaatgctata
     1921 catatagctg ctgaaatatc agaaatgttc ccagcagatg aaagaccaga aacaacagaa
     1981 ggatatgaag gattttggca tttaaattct ataggtggaa atgtcgagaa tgtaagtatg
     2041 gcttatatta ttagagacca ttgtaaagaa aaatttgaaa atagaaaatc tattatgata
     2101 gagaatatag aaaaaataaa taagaaatac gataatagag tggaattgga tttaaaagat
     2161 tcttattaca atatgaaaga aaagattgag ccagttatgt ttatagttga tatagctaaa
     2221 gaagctatgg aggaattagg tataaaacca cgtttagttc ctgtaagagg tggtactgat
     2281 ggagcaagat tatcttttaa tggattacct tgtccaaata tatttactgg tggattaaac
     2341 ttccatggga aaaatgagtg tataccagtt agttctatgg agaaagctac taaactaatt
     2401 gtaagaatag ctgaaaaata tgctgaaaga gtttagttaa aaggtattta aaaattaagc
     2461 atttaaagtt ataatagcta tatcattctt tataagtcaa gctgattata agcttgtgtt
     2521 gtatttaaaa tggtatagct atttttgtaa ttatttttat aaaagataat tatttttagt
     2581 ttagggtatc aataaaaagt atgaatatca ataggtatta acatttaaaa gggggaattg
     2641 tattgaaaat taaatctaaa aaaataatag catttgcact ttcacttggg attatgttgc
     2701 caagttgtgt atatgcagac actattagta aaaatcaaaa tgcacaggta gatatgaaca
     2761 attatcttca aggtagaaaa tctccaaagg aggaaaaact tattggtgaa gatagatttg
     2821 atacagcgat aaaaatatct caaagtggtt ggaataatgg ttcagaaagg gtgtttctag
     2881 ttaactcaaa ttcattgcca gatgcattgg cttcaactcc tttggcaagt aaattggatg
     2941 caccaatact gcttacaaat aaaaatagta taccatataa tgtaatagag gaaataaaaa
     3001 ggcttaatcc agcagaagtt attttaattg gaagtgaagg agctatttca tcaagtgtaa
     3061 aagaatctct tgaagatatg gaagtattag tcagtagaat cggtggagca gatagagaag
     3121 aaacatcttt acttcttact agacagctag atgatactgg tgatttaggt gtctcaaaag
     3181 tggcagtggt taatgggtat aatggactgg cagatgcaac ttcaatatca agcccagctg
     3241 caagtgataa tacagctata atatatacag gtaaggatag cataagaagt gaggcaaaga
     3301 attttattac acaaaactca actaagacat acatagtagg tggggagtac agtataagta
     3361 aaaaacttga aggacagtta gtaaactcag aaagactagc agggacagat agaaaagata
     3421 ctaatgcgaa agttttagaa aagttttatg gaaaatcaag taaggttaat aatatgtatt
     3481 ttgcaaaaga tggaagtggt agagaagctg atttagttga tgggcttgct gctggagtat
     3541 tagcatcaaa aacaaagtca cctgtagtac ttgcaagtgg aagtttaagt agtgcacaga
     3601 aaagttttat aaaaaaagta aaggctgata aatttgtaca agttggtgga ggtaaaaata
     3661 gtaaaccata tgcagaagca ttagccttac agtaatattg tgtaaatcaa aattaatata
     3721 taataatagc aagtaaacca agttgcactt caatatatat aataaaataa gtaaactaag
     3781 ttatatttca gtataatata taatgaaata agtaaactaa gttatactcc aatataatat
     3841 ataatagaaa caagtaaact aagttatatt tcaatatagc atataataaa acaagtaaac
     3901 cagattctat ttaattagaa gtttaaggta ttgtacttaa acttctaatt gagatagaaa
     3961 taaaatggtt tacttgtttt tattatgagt ttaataaatt aaagcttatt ttatttttat
     4021 taaaaattta ctaatcattg atatttatta tatttccagt attgatgtca attaagttta
     4081 tatcaagttc aagctcatcc tcatcatcag tttttatgat atctataatt gcgacagaat
     4141 ggcttccatc ttttgggata gaagtacttc ctggatttat tactattaaa ttatcatcca
     4201 tgtatagctt ttttacatga gtatgtccta aaactaatat gtcaccaccc attttctttg
     4261 ctttgtcaat aatttcttct tctgattcta tataaccgtg attcaatata attctaattt
     4321 caccaaattg gctcatgaca taaggactct gaataggatg ttctataacc atctggtcaa
     4381 catctgcatc acagttccct tttacaatca caatgttttc taatttattt aatgtctcaa
     4441 taaatttttt aggattatat ccttctggaa tatcatttct aggtccatga tataagacat
     4501 ctccaccatg taataaaaca tcacaatcag ataatacatt taacgctttt tcaaagtaaa
     4561 gtaagctacc atgagtatca ctcattattc ctattttcat attaaaaatc ctcctaagta
     4621 cagtctataa aaatttttca gatactctat gccaaaactc atagtttgac attctaagta
     4681 attttatttt tgcatcagat atactaactt ttatattttc gatttgtcta aatctgtgct
     4741 caactccatc aacgacaatc aatacagaat cttcaaatct atattctggt gcaatcttta
     4801 taacagattc atttgaacat attatacttg atgtaaagca cctatatgca ttggtattta
     4861 tcggatgtaa aggtgtaatc tgcataagtt caagatttat atcaacaata ctacctccag
     4921 cagaatagtt ataggctgtt gaacctgtgg aagtagaaat aatcatacca tctccactaa
     4981 aattttgtat atgtttatta tcaaggctta aatttaggtg tatagtccta gacttgtctc
     5041 ctcttataac aacctcattt acagcaagca tattagaacc actagtagtt gtatatactt
     5101 ccgcattaag aagtgacatt tcttgaataa tataatcttt ttttgtataa gcttcaataa
     5161 agctatcaat cttatctggt aaaatatcag gaaaaaaccc cagatgacct gtatttattc
     5221 ccattatggg aatctcagga aaatcgaaat ctctaactgt tcttaaaaaa gagccatcac
     5281 caccaattga tataataagc tctgtgtctg gataaatatc tgaatacact tcaaagccag
     5341 cattgataag ttttctagtt aagagttctt tagtctcaag ggacttattt aattggtttg
     5401 tatttatagt tataattctt ttcatatatt cacctcttca tactttgtat tataacatag
     5461 ttttataatt cttaatttat aaatttataa atgacctttg taatgaatgt gttaaaatat
     5521 gtataggtgg gtataaaaaa atgcataaga ggtgaagatg tgtttagcat aaggaatatc
     5581 acaaaaaaat taggaaaatt caagttaaat aatataaact tagagttaaa ggaaggggat
     5641 attgttggga ttataggacc aaatggttcg ggaaaaacga ctttaataaa aattataatg
     5701 gggattattg atgcagatga aggtgaaatt gaactttgta atgaaactat tgaaaattct
     5761 ccaataagct ttaaaaacaa tattggtttt gtatatgatt ctttacaatt ttatccgcat
     5821 ttaaaggtta aggaatttag aaaaatagtt tctttatttt ataagaattt tgatagagaa
     5881 aggtttgatg aataccttaa taagtttgat atagaagaaa atatgcatat tgaaaattta
     5941 tctaaaggtc aatctgaaaa gcttatgtta tcaagcgcac ttagtcacaa tgctaagtta
     6001 ttgattttgg atgaaccaac tgctgggata gacccaattg taagaactga aataatgcag
     6061 tatttacaag attttgtgaa aaatggaagt agtagtgtaa taatttctac tcataatact
     6121 gataatttaa ttaaaattgc cgattatctt gtttttataa atagaggaaa tcagattttt
     6181 actgttaaga aagaattaat agaacaagaa tataagataa ttagggcaaa taaagcagag
     6241 ttagaagcaa ttaaagaaag catagttggt gtgaaagagt ataaatatta taatgaggca
     6301 cttgttaaag ttggagatag cttgaaagta aaaagcctat tgatagaaat tgataagcat
     6361 aaggtcaaaa atcctactat agaagaacta atgtattatt atgtaaatga ggtgagatga
     6421 tgttcaattt aataagaaaa gatttaataa tagggattag ttcagatggt atccgtaatt
     6481 taaagtacat attgctattt tttgtgtttt attttttcct taattcaatt tcatattata
     6541 cagtaagtat tgttgttagt tatttgatat ttataaatac atttgagtgt gattatgaaa
     6601 atgattcaag gatttttata aggagtatgc cagttagtat agaagatata gtttactcaa
     6661 aatatttatt aggtgtagga ttaataattt ctgtaacaat aattgtgtcc ttactaagta
     6721 aattgacatc actagtgttc tttagaaata tggttttaaa tgatgtattt ttttctgtaa
     6781 atatattttt agcaattttg acgattttac tgcctttaat ttttaagttt ggatatggaa
     6841 aaatgaaagt ttgtggtttt atagtaagta ttttaattta ctttgtatat ggaagtcttt
     6901 tgaagatgat tagtatgatt gtatatcaag taaagcattt taattatagt aaagttggag
     6961 gagtatatct ttcaaattat gtaactgata tagctaatac taagtatata aatttatata
     7021 gtatgacatt tttaacaata ataattttta taatatcaat gtatttttct attaagattt
     7081 caaaaaagaa taagtttaat tattaaaatt ttaattaata ttatagcaca atagcacaaa
     7141 atataaggag actaaaaatg aagaagttta taaaagtttc attttttatt ttggggattt
     7201 ttacactatt catagctata aataaattag tatatagaga tatggtaagt gtcaaagatt
     7261 atgaaataaa tcatttagag gaaattgttt ttgattttga tagcaggaga agtagagatg
     7321 ttggtactat tgtaagattt aaaataagca ataatagtaa atatagttat aaattaaata
     7381 gtgctaaaat gaagtttgaa aattgtgtgg aaaataaaaa gggaatgaat aagtgttctg
     7441 catatgtaaa tgtagattta tttaatgaaa aaaattctat aactgaaagg actctgaaat
     7501 atggaataaa accaaataat caaggttatg ttgattttgt aattccaaaa ggactaaatt
     7561 ttgattatag gtactttaat gaatctggta tgagtgtaga atataaagga gaatatattg
     7621 tcaatattcc aatggcaaaa ggactttatc tagtagttgg aaaaaataat gatacatgga
     7681 atgctaatat tatggaataa tttgaaaaat aaatcaatta tagatataat taagaataat
     7741 atgaaattta taaaagttca gactaggagg ggtatttaat gtcaataagt aatgtaagag
     7801 agtattttaa acaatttggc aaggaagaca gtatattaga atttgaacaa tctagtgcta
     7861 ctgtggaact tgcagctgaa gcagctgggg ttattccagc aagaatagct aagacactct
     7921 catttaaaat aggagatgat gcaattttaa ttgtgacagc tggtgatgct aagattgata
     7981 ataagaagta taaggctgaa tttaattgta aggctaaaat gttgacacca gaagaagtct
     8041 tggagtttac tggacatgct attggtggag tctgtccatt tggtttaaaa aattctataa
     8101 aagtctattt agatgattca atgaaaagat ttgatacagt atttcctgca tgtggcagta
     8161 gtaactctgc aatagaactt acatgtgaag aaatggagaa gttttctaag tgtgaaaaat
     8221 gggtagatgt ttgtaagaat tggtaaaaaa atatagtaaa ctatagtagg aattagtaaa
     8281 aaactacctc tgtatacaat ctatacagag gtagtttttt tgtgtaaata tttataaatc
     8341 taagaggaaa atctaattta aactaataat ccaaaataaa gttaaaaaat tctgattttt
     8401 tttcgaaaca ataaaaaaat gacaaaaaag tggaaaaaat gtaataaata ttgttaaaaa
     8461 aataattaga aattattgaa aaattatcct ttgagtgata ctattataga tgtaagatat
     8521 ttaaaaataa aaaatagtca gataaaaata cgattattta gctctattaa aataattttt
     8581 atacgataaa aatcttctgt acatggctaa aaaacagatg tacagacaat atttaatgtt
     8641 taatataccc taatataaat atcgtaaaca aattatttta tctgtaacca gtgtgaaaga
     8701 aatcttattc taacttgaaa tttcacaaaa ttccaaaaag cgagattttt tttaagtata
     8761 gttgatttta aattaacaat ttatgattaa attaatgtca taattggtta gtataatcac
     8821 atataaatat agagcgtagt ttgattaagg ttataaattt ctcgacgtat attcaaatac
     8881 cttattatag atgttgccga gattttaatt aataaatgaa aataaaatat ttataaaata
     8941 ctgaggaggc tattatggat ttaaattcta aaaaatatca gatgcttaaa gagctatatg
     9001 taagcttcgc tgaaaatgaa gttaaacctt tagcaacaga acttgatgaa gaagaaagat
     9061 ttccttatga aacagtggaa aagatggcaa aagcaggaat gatgggtata ccatatccaa
     9121 aagaatatgg tggagaaggt ggagacactg taggatatat aatggcagtt gaagaattgt
     9181 ctagagtttg tggtactaca ggagttatat tatcagctca tacatctctt ggctcatggc
     9241 ctatatatca atatggtaat gaagaacaaa aacaaaaatt cttaagacca ctagcaagtg
     9301 gagaaaaatt aggagcattt ggtcttactg agcctaatgc tggtacagat gcgtctggcc
     9361 aacaaacaac tgctgtttta gacggggatg aatacatact taatggctca aaaatattta
     9421 taacaaatgc aatagctggt gacatatatg tagtaatggc aatgactgat aaatctaagg
     9481 ggaacaaagg aatatcagca tttatagttg aaaaaggaac tcctgggttt agctttggag
     9541 ttaaagaaaa gaaaatgggt ataagaggtt cagctacgag tgaattaata tttgaggatt
     9601 gcagaatacc taaagaaaat ttacttggaa aagaaggtca aggatttaag atagcaatgt
     9661 ctactcttga tggtggtaga attggtatag ctgcacaagc tttaggttta gcacaaggtg
     9721 ctcttgatga aactgttaaa tatgtaaaag aaagagtaca atttggtaga ccattatcaa
     9781 aattccaaaa tacacaattc caattagctg atatggaagt taaggtacaa gcggctagac
     9841 accttgtata tcaagcagct ataaataaag acttaggaaa accttatgga gtagaagcag
     9901 caatggcaaa attatttgca gctgaaacag ctatggaagt tactacaaaa gctgtacaac
     9961 ttcatggagg atatggatac actcgtgact atccagtaga aagaatgatg agagatgcta
    10021 agataactga aatatatgaa ggaactagtg aagttcaaag aatggttatt tcaggaaaat
    10081 tattaaaata gataaatatt aaaatataca caataggtcg atattaaaat agatatggag
    10141 gaaggattta tgaatatagt cgtttgtata aaacaagttc cagatacaac agaagttaaa
    10201 ctagatccta atacaggtac tttaattaga gatggagtac caagtataat aaaccctgat
    10261 gataaagcag gtttagaaga agctataaaa ttaaaagaag aaatgggtgc tcatgtaact
    10321 gttataacaa tgggacctcc tcaagcagat atggctttaa aagaagcttt agcaatgggt
    10381 gcagatagag gtatattatt aacagataga gcatttgcag gtgctgatac ttgggcaact
    10441 tcatcagcat tagcaggagc attaaaaaat atagattttg atattataat agctggaaga
    10501 caggcgatag atggagatac tgcacaagtt ggacctcaaa tagctgaaca tttaaatctt
    10561 ccatcaataa catatgctga agaaataaaa actgaaggtg aatatgtatt agtaaaaaga
    10621 caatttgaag attgttgcca tgacttaaaa gttaaaatgc catgccttat aacaactctt
    10681 aaagatatga acacaccaag atacatgaaa gttggaagaa tatatgatgc tttcgaaaat
    10741 gatgtagtag aaacatggac tgtaaaagat atagaagttg acccttctaa tttaggtctt
    10801 aaaggttctc caactagtgt atttaaatca tttacaaaat cagttaaacc agctggtaca
    10861 atatacaatg aagatgcgaa aacatcagct ggaattatca tagataaatt aaaagagaag
    10921 tatatcatat aatttaaggg gggataaaga atgggtaacg ttttagtagt aatagaacaa
    10981 agagaaaatg taattcaaac tgtttcttta gaattactag gaaaggctac agaaatagca
    11041 aaagattatg atacaaaagt ttctgcatta cttttaggta gtaaggtaga aggtttaata
    11101 gatacattag cacactatgg tgcagatgag gtaatagtag tagatgatga agctttagca
    11161 gtgtatacaa ctgaaccata tacaaaagca gcttatgaag caataaaagc agctgaccct
    11221 atagttgtat tatttggtgc aacttcaata ggtagagatt tagcgcctag agtttctgct
    11281 agaatacata caggtcttac tgctgactgt acaggtcttg cagtagctga agatacaaaa
    11341 ttattattaa tgacaagacc tgcctttggt ggaaatataa tggcaacaat agtttgtaaa
    11401 gatttcagac ctcaaatgtc tacagttaga ccaggggtta tgaagaaaaa tgaacctgat
    11461 gaaactaaag aagctgtaat taaccgtttc aaggtagaat ttaatgatgc tgataaatta
    11521 gttcaagttg tacaagtaat aaaagaagct aaaaaacaag ttaaaataga agatgctaag
    11581 atattagttt ctgctggacg tggaatgggt ggaaaagaaa acttagacat actttatgaa
    11641 ttagctgaaa ttataggtgg agaagtttct ggttctcgtg ccactataga tgcaggttgg
    11701 ttagataaag caagacaagt tggtcaaact ggtaaaactg taagaccaga cctttatata
    11761 gcatgtggta tatctggagc aatacaacat atagctggta tggaagatgc tgagtttata
    11821 gttgctataa ataaaaatcc agaagctcca atatttaaat atgctgatgt tggtatagtt
    11881 ggagatgttc ataaagtgct tccagaactt atcagtcagt taagtgttgc aaaagaaaaa
    11941 ggtgaagttt tagctaacta atttcttggg gagggtatta tgagtacaag tgatgttaaa
    12001 gtttatgaga atgtagctgt tgaagtagat ggaaatatat gtacagtgaa aatgaataga
    12061 cctaaagccc ttaatgcaat aaattcaaag actttagaag aactttatga agtatttgta
    12121 gatattaata atgatgaaac tattgatgtt gtaatattga caggggaagg aaaggcattt
    12181 gtagctggag cagatattgc atacatgaaa gatttagatg ctgtagctgc taaagatttt
    12241 agtatcttag gagcaaaagc ttttggagaa atagaaaata gtaaaaaagt agtgatagct
    12301 gctgtaaacg gatttgcttt aggtggagga tgtgaacttg caatggcatg tgatataaga
    12361 attgcatctg ctaaagctaa atttggtcag ccagaagtaa ctcttggaat aactccagga
    12421 tatggaggaa ctcaaagact tacaatattg gttggaatgg caaaagcaaa agaattaatc
    12481 tttacaggtc aagttataaa agctgatgaa gctgaaaaaa tagggctagt aaatagagtc
    12541 gttgagccag acattttaat agaagaagtt gagaaattag ctaagataat agctaaaaat
    12601 gctcagcttg cagttagata ctctaaagaa gcaatacaac ttggtgctca aactgatata
    12661 aatactggaa tagatataga atctaattta tttggtcttt gtttttcaac taaagaccaa
    12721 aaagaaggaa tgtcagcttt cgttgaaaag agagaagcta actttataaa agggtaaaaa
    12781 tctaattaaa aatatatttg ctgttaacta gattaaatag tattttaatc tagttaacag
    12841 aatataaata ctaaaataac aggaggttat aactatgaaa ttagctgtaa taggtagtgg
    12901 aactatggga agtggtattg tacaaacttt tgcaagttgt ggacatgatg tatgtttaaa
    12961 gagtagaact caaggtgcta tagataaatg tttagcttta ttagataaaa atttaactaa
    13021 gttagttact aagggaaaaa tggatgaagc tacaaaagca gaaatattaa gtcatgttag
    13081 ttcaactact aactatgaag atttaaaaga tatggattta ataatagaag catctgtaga
    13141 agacatgaat ataaagaaag atgttttcaa gttactagat gaattatgta aagaagatac
    13201 tatcttggca acaaatactt catcattatc tataacagaa atagcttctt ctactaagag
    13261 accagataaa gttataggaa tgcatttctt taatccagtt cctatgatga aattagttga
    13321 agttataagt ggtcagttaa catcaaaagt tacttttgat acagtatttg aattatctaa
    13381 gagtatcaat aaagtaccag tagatgtatc tgaatctcct ggatttgtag taaatagaat
    13441 acttatacct atgataaatg aagctgttgg tatatatgca gatggtgttg caagtaaaga
    13501 agaaatagat gaagctatga aattaggagc aaaccatcca atgggaccac tagcattagg
    13561 tgatttaatt ggattagatg ttgttttagc tataatgaac gttttatata ctgagtttgg
    13621 agatactaaa tatagacctc atccactttt agctaaaatg gttagagcta atcaattagg
    13681 aagaaaaact aagataggat tctatgatta taataaataa gaatttggga attaaaagtt
    13741 taaataaaat tgtttaaaaa acaatttcgg atatatgaaa aatctaattt aatgggggta
    13801 atgaatatga gagaagtagt aattgccagt gcagctagaa cagcagtagg aagttttgga
    13861 ggagcattta aatcagtttc agcggtagag ttaggggtaa cagcagctaa agaagctata
    13921 aaaagagcta acataactcc agatatgata gatgaatctc ttttaggggg agtacttaca
    13981 gcaggtcttg gacaaaatat agcaagacaa atagcattag gagcaggaat accagtagaa
    14041 aaaccagcta tgactataaa tatagtttgt ggttctggat taagatctgt ttcaatggca
    14101 tctcaactta tagcattagg tgatgctgat ataatgttag ttggtggagc tgaaaacatg
    14161 agtatgtctc cttatttagt accaagtgcg agatatggtg caagaatggg tgatgctgct
    14221 tttgttgatt caatgataaa agatggatta tcagacatat ttaataacta tcacatgggt
    14281 attactgctg aaaacatagc agagcaatgg aatataacta gagaagaaca agatgaatta
    14341 gctcttgcaa gtcaaaataa agctgaaaaa gctcaagctg aaggaaaatt tgatgaagaa
    14401 atagttcctg ttgttataaa aggaagaaaa ggtgacactg tagtagataa agatgaatat
    14461 attaagcctg gcactacaat ggagaaactt gctaagttaa gacctgcatt taaaaaagat
    14521 ggaacagtta ctgctggtaa tgcatcagga ataaatgatg gtgctgctat gctagtagta
    14581 atggctaaag aaaaagctga agaactagga atagagcctc ttgcaactat agtttcttat
    14641 ggaacagctg gtgttgaccc taaaataatg ggatatggac cagttccagc aactaaaaaa
    14701 gctttagaag ctgctaatat gactattgaa gatatagatt tagttgaagc taatgaggca
    14761 tttgctgccc aatctgtagc tgtaataaga gacttaaata tagatatgaa taaagttaat
    14821 gttaatggtg gagcaatagc tataggacat ccaataggat gctcaggagc aagaatactt
    14881 actacacttt tatatgaaat gaagagaaga gatgctaaaa ctggtcttgc tacactttgt
    14941 ataggtggag gaatgggaac tactttaata gttaagagat agttttagat tataatattt
    15001 acaaattaaa tatttaatta tgaatttaac ctgtaagaat aactatttgt tattcttaca
    15061 ggtttttata gttaaaaata attattatct attaaacaaa aatagtgtaa aatatataat
    15121 atcatgattt tgaagatata aaaagtagga agatataaaa gaaagtaggt aaaagtttgt
    15181 ttaaaaaaga gaatcaaaga tataatctta tatcgtatac aaatgaagaa gaaatgactt
    15241 taaaagaagt tttattggac aagctaaatt tctcagttag gtcattatca aaaatgaaga
    15301 gggaaaagag tgttttagta aatggagtat ataaaaaacc gagtttaaag gtgtatagtg
    15361 gtgatttaat agaagtaaaa atatacgagg aaaaagcaaa ttttgaacct caagatttaa
    15421 atttacagat aatttatgat gattttgata taattatggt gaataaacct ccgtttatgg
    15481 tagttcaccc aacaaaaagc cactatgata aaacaatagc aaatggtatt agttactata
    15541 tagataatca aaaagaaaac gtaaaaataa gatttgtaaa taggttagat atgaatactt
    15601 cagggttagt tatagtagca aaaaatgctt atgcacatca tactttgtct acagcaatga
    15661 gtgaaaataa agttgaaaaa aagtatataa cagtagttga tggaattata aaagaaaatg
    15721 agggaactat agatgagcca atttatagac ctatagaaga ttcaataaag aggataatag
    15781 atgaaagagg acagtcatct gttactcact ataaagtaat agaaagacta gaaaatgcaa
    15841 ctgtattaga agtaagctta gaaactggta gaacacatca aataagagta catatggctc
    15901 atataggtca tggaataata ggtgatgagt tgtatggtta tgtagatgaa gagctaataa
    15961 ataggcaagc acttcatgca tataagttag agtttgaaca accaagaaca aaagaaaaat
    16021 tgaaatttaa agcagatata ccagaggata tgaaagagtt aatatcgaaa ttaagataat
    16081 ggggtgatat atatgataat acataaattt ataatacatg ttttggacaa aaatagtgat
    16141 gttccaatat taaatgactt tgaaggcaaa gttaatcaag aagtagatgg ttttttccag
    16201 aaagctataa aaagaattgc aaaagatgag gatttaagaa aaggtgtttt taaagattat
    16261 aatgacaatt taattaaaaa ctgttgtgaa caaataatat atgatgaaag tacttttttg
    16321 aaaaattcta aagaaattgc atcttacttg tttgacgtta tgaaaattaa tgctgcacta
    16381 gaatcttgtg atttggcaat ttgtttatac actattaaag atgaaaagag tgtagcaata
    16441 ctgaaattag attataaaaa gttgtacact cattcaattg aatttgtgga tgataaattt
    16501 aatattcaaa tggtttcaaa tgaaattggt atcccagaga ctttaagaca aaagcaagga
    16561 gctttgatta gtctaagtgg tataaatgat gagtttcatc taaggctttt agataaggat
    16621 gctgaaaaag aagggtcaga atctaagttt gtaacagaat ttttaaatgc taaaaaaata
    16681 gatgatgata agtacaagac caaggtattt aaaaatacag ctgaaaattg gataaccaat
    16741 gctcttagta atgatataaa gcaagctgaa gatgtaagaa gtatattaaa ttatacactg
    16801 aaggaaaaac atgaagttga tataaatgac tttgttgata attcaattaa agatgatgag
    16861 ttaaaggata gctttaaaga gcatatggaa gaaaaaggtc ttgatgaaag ttttagtata
    16921 gataaaaaat gggttgagaa aaagcttaaa aaaagaagta taaaaacgga taatggtttt
    16981 gacataaaag ggaatctaac tgattttgaa gacccaatga agtatactgt aaaacaaaat
    17041 caagatggaa ctatagatat aataataaag aatgttacgt tttatgaaga aaagtaattt
    17101 atatacttag tatataaatt aaatataaga gcgtgtattt tatatattgt aaataaatgt
    17161 aatctagtct gtgcgaactc taatataatt tattatgtag aagtttacac agactattta
    17221 tatttgtaga caaaacgata ctatttgttt tcttcgatat atttacatat atcaccaata
    17281 catttaaagt tctcagcttc ctcgtctggt atttctattc caaattcatc ttcaagggcc
    17341 attataattt caacagcatc taatgaatca gcttctaagt cgtccattaa agatgcttcc
    17401 atagttatct catctaaatt gtctacccct aattgttctg ctattatttc ttttattttc
    17461 tcaaacattt taagcctcct aaagcagttt taattaattt taatgttagt ttaatataat
    17521 atcaagaatt tttcaatatg gaatctataa aaaataataa aattttataa atgtagtaat
    17581 aaagtattat ttgatgaata aactttattg ataacaaata taacttagga ggaaatgcta
    17641 tgaaatatgt aggactatta ctaacttcag taggaatgtt tttacttata gcagtaaatt
    17701 tttattataa ttcaataact ttagatatgc aacgtataga ggattatgta atggagacca
    17761 atttaatcct tgaagatgta gctgaaaagg agagttatgt atctaatgaa aaagaagact
    17821 acatatcaag gttaatgcat gtaaagaaag gaatagaaaa ttcaaaaaca tccttcttga
    17881 tagaaagata taaggagtac aaaataaaat caatagaaag tttaatctat actatatcag
    17941 aggagaaaaa agattatctg gatgaagttg atagatacaa taaacttggt gaaaaagaaa
    18001 taaataaact aatcaataaa aactttttag aagtaactta tctgtctatt actacatata
    18061 tttaattaaa gagacaaaac ttagtctctt aatgattcaa ggaggattag ttatgacaaa
    18121 attagaagac aatatggaac gtttctttgg aggatgcgaa ggattttttg ggaataaaat
    18181 gttgataata atagttatag tattcttact attatgcaca gacattttag aagacttatt
    18241 atgcgatgac aatatatgga tatgggttat attaatagta ttgttattat ttaacttcga
    18301 tgatggttgt tgttgctaaa aaatattgat tattaaatag gtaaaaccag taacttatac
    18361 aagttatggt tttacctttt ttagttcaaa ttaaaaaatt taaaaaaata gtaaaaatac
    18421 attagcaaaa tggaaaccaa atgcatagta tatgttagga gcgaaaagct caacaaaata
    18481 tcaacaggag gtaacgtata atgttagata gattctttgg agaagaagga tgttttggtg
    18541 gaggaggatg gtggataata gtactattct tcctattctt agcttttgga gatagctggg
    18601 cagaatttga cataatggca tggataccat tcctaatagt tttattaata ctttgctgct
    18661 gtggtggatg cttagaataa gaacacagtt attaaaaaaa ggagggcttt gttatgttag
    18721 atagattttt tggagaagaa ggatgctttg gtggaggagg atggtggata atagtactat
    18781 tcttcctatt cttagctttt gaagattgct ggagagacat tgacataatg tcatggatac
    18841 ctttcttaat attattgcta ataacttgtt catgtggtgg attctttgat ggtgaagtag
    18901 gttgcggctg ctaatactta aatcattaga ataaaaaaat gctggtattt tataccagca
    18961 tttttttatt ttatttttta atatgattta aattaagaat gaatattatt aaatttgttg
    19021 aatatagggt ataatataaa atataatgta tttaaatgta cttgctttta tggtaaaata
    19081 aaaacttata taaatgtagt taaaatagga accttcaaaa atttggttca gttttagata
    19141 aatttagtaa aataaggagg atgaagatga aaggcaatat aacgataaaa gatgttgcta
    19201 aacaagcagg agtgtcaata tctactgtat ctagagttat aaatgattca aaacctgtaa
    19261 ctgatgaagt caaacaaaaa gttttagagg ttataaaaga gactggatat ataccaaatc
    19321 cacttgctag aagcttagta acaaagaaga gtcaattaat aggggtaata gttccagaag
    19381 tttcagattc ttttgttaat gaggtgttaa atgggataga agaggttgct aaaatgtatg
    19441 actatgatat tcttttagcg aatacatact ctgataagga acaagaactt aagagtataa
    19501 atctattgag agcaaaacaa gtggaaggta tagttatgat ttcatggata gttgaacaag
    19561 aacatatcaa ctatatacaa aattgtggaa taccagcgac atatataagt aaaactgcta
    19621 gaaattatga tatatataca gtaagtacta gcaacgaaga agctactttt gatatgacag
    19681 agcatcttat aaagaaaggt catgaaaaga tagcttttat aatgacgagt aaagatgata
    19741 ctgttttaga aatggaaaga cttgctggtt atgagaaagc actttcaaat aacaatatag
    19801 aattagacaa gagtttgatt aagtatggtg gaactgatta tgagagtgga tacaatagta
    19861 tgaaagaact attagatgat ggaataatac ctcatgcggc ttttgtaaca ggtgatgagg
    19921 ctgccatagg tgctataaat gctatatgtg atgctggata taaggttcca gaagacatat
    19981 ctgttgcagg atttaatgat gttaagatag ctagaatgta tagacctaaa cttactacag
    20041 tatatcaacc tctatacgat atgggagcag tagcaataag aatggttata aaattaataa
    20101 ataaggaatt aattgaaaat aagaaaatag aattacctta tagaattgtt gatagagaaa
    20161 gtgtcacaga aagaaaaaaa taaaactatt aaagtcaatc aggtagtgcc taaggcacta
    20221 cctgattttt tatgtcaata atatcaaatt taataataga ttaactaaaa ataattatct
    20281 aattacttaa atagtttatt tttatctatt catgagggtt agacttttta gttttcttaa
    20341 ataaactttt agattttttt tgttgactac ctggtgtatt ttttggcgca ggtgaatttg
    20401 acttaccttt agtaggttta gccttcttag ttttagctgg tttagtatct ctttggtttt
    20461 ctgcgtctga gattttttcc attatattat ccagtttttt gcgttgattt tcatctaaaa
    20521 atgctttgaa ttttgatact acttcatctc tgccatctag gttcttacct atttttataa
    20581 gttcctccat aagttcattt tctgattttc ctttgtaact atcagccatc ttttctattt
    20641 tatccttgtc aatatttttt tgtttagcca ttttttctaa tgactctgta tctaattttt
    20701 tcataaaaaa acccctttct ataaatatat attgaattat attcatacta tttatatata
    20761 gaaataatat tgaagtggtg aattgtgagt gatatataat ataaataaaa attcttatag
    20821 ataatatata tgcactgagc tttaaaaatg gaacattaaa atatgaaaaa catactttaa
    20881 tatagggcaa ttatataata taagggggaa ttgtcttgaa taacttacta tttttgattg
    20941 gagtgattgc attgagtaat ggtaatatct ctatatttga tgataaaaat aaatcaaaag
    21001 gaaataatag agttagaaaa aacaaaaaaa atgcaaatga taaactttca cagtctacac
    21061 atggcaattt aaggtttgat gcaaatgata taaaaagagg tttaaggctc atggatttaa
    21121 gtgaagaaga tttagaaatg ggaatggaaa taataacaag aactaaaaaa tacatgtcaa
    21181 gagatgaaag aaaaatacta ataaaaatag aaagtgtttt agatttagtt agaggaataa
    21241 aaaaattaaa taatatagat atggtagata cagaagatga aacggatttt ttcagaaaaa
    21301 tggatagtga tgataaaaag aatatgatga taaaggaaat tatagatgta ttcccagaaa
    21361 aaagaaaaaa ttcagttgaa aaagctatag gaatgaaaaa gaagatagat ttatttgcag
    21421 aattattttt accagatgac tttggtgaag gtggatttag tttgagctct ttggcaaata
    21481 taaacaatct tggtagtatg aataatctca aattgttagg aagtctttta agaggtgatg
    21541 atagtaataa tgatgatgaa gacaataatg atgagttaaa ttatgaagac gaagaattag
    21601 attgtgaaga caaagaattg gatgatgatg agttaacaga tgaaggtgaa atggaggata
    21661 atgaagatga attagtagaa gataagaaca caggctatga aaatatagaa aatgatgaag
    21721 atatagtata cgatgaggaa ctatacaaca ttaatgagca agaatccgaa tacaattata
    21781 atgaaaaaaa tactaaaact agagctaaga aaaaacgata attaactaaa tacataaaga
    21841 aaaaaggaag aaaaagtaat gatagaataa gattatctca attactatat aaaaccactt
    21901 taaaaaattc aaaatttatt atataataat aatatgaaat cctgaaacat acgataaggc
    21961 ttttttaaat tcaacctaca aatgaaatac gggagaccgt gtctggtaaa ttataacatg
    22021 cctagttagt ttggacgtta aaggttactg gcaagtcacc cacctgggag tgcctagggt
    22081 gtgaatttac ttagccaccg gtgtttcggg acaaaaaaag tgctactatt tcaaatagta
    22141 gtactttttt tattaatatg catttttcta ctaaatacgc ctaattattg ataaattcaa
    22201 aaagcaagta acaattaaga ttataaaagc atagtattaa ttaggcttca aaaagccaaa
    22261 gacttaataa gaatcgctta cagggagggc aatgaaaaat gcaagattat aaaaaaaata
    22321 aaagaagaat gatgaatcag ccaatgtcta caatgaatga agaagaagtg tatacagatg
    22381 aaataaattc agaagacatg agaggtttta aaaaatcaca ccatcataat ggatgtaata
    22441 ctgataataa gtgtgagtgc catgatgatt gcaatccatg caacccatgt aatccatgta
    22501 aacctaaccc atgcaatcca tgcaaaccta atccatgtga tgacaattgt ggatgccatg
    22561 acaattgtaa atgtgattgt gaaccatgtc aaatggattc agatgaatgt tttgaaaaca
    22621 aatgtggacc agaatgctgt aatcctatat ctccaagaaa cttctctgta tcaaatgcag
    22681 tgccatttgc aatagaggct aatagaatat ttgatactat gcaattccaa acatttacag
    22741 atgcaacagg accaaatgga gagccattaa cttttgaaac agaagtagta gaagtatttg
    22801 gttcagttcc aagtgcaggt caagcaagtg taactataga aaaaatatgc ttaagtaatg
    22861 atggaatagt tatagacaca ggaatgacaa ctttagaaga tttcgattta gacccattag
    22921 gagatatagt aggaagaaac tgtgaaacaa cttttgaatt tgcagtttgt ggagaaagaa
    22981 acgctgagtg ctgtagacaa ggaaaaggca aatcagtagc ttataaacaa agaggattaa
    23041 ctgtagcagt tcgtaattta gtactagagc taagaggtag atgtggatgt acagagttcg
    23101 ttgcattagc tttcccagca gttagagcag gaggtggatg taagagaaga gttgattatg
    23161 tagaatttac ttttaacaca ctttcagcac caatatgctt gccagctgac ggaagagctg
    23221 ttactttaag acaagaatat caaactaact taactgtaga ttgtatagga aaatctatat
    23281 taaaattaga atgcaacgaa tgttgtgaac ctttctatga attaattata ccaaatgata
    23341 tagatttagt actttgctta caagaaacag ttagcacatt aataagtgaa caaatagtag
    23401 ttttagcatc accaaatcca atccaaccaa gacttgttga tactttctct aaagtatgtg
    23461 atttttcgca atgtggacct aatcatggaa gtggaaagcc aagttgccac agatagatag
    23521 gtaagatata tagacagttt aaattataaa taaaaatcat ctaaggaagg ctaccaaagt
    23581 gtataattta cattggtagc cttcacgttt ttataactag cgtatagatt ttagtgattt
    23641 aaaaagcact atagtatgta atagacaagt gaagattgat actatcgtca ttgtgtatat
    23701 gtatgcataa atattaagtc ctggaattgg aagaaataca tataaagcta ttagttgaat
    23761 taacatgcca gtaatagtat ttatacttga agctaattct tttcttattg aatgtaaaat
    23821 gccagataga gtttgattta gggacataaa tagtggaact aaaaacatag cttttagata
    23881 ttctcctgca agtgtatttt taaatactaa tatacataaa tcttttccaa agaaataaaa
    23941 aaatattgat gatagtatgc ctacaaataa agtaagaagt atggagtatt tgattttttt
    24001 aattacactc ttacgctttc ttagagccat ttcctgtgaa atagttggta ttaaatttac
    24061 aataagcgct gaaccaacag taaaaggtaa aaatataaaa ggcattacca tcccactgac
    24121 aataccatac atacttaaag attgttgata agtcattcct gataatgcta atcttgatgg
    24181 tatcatcata gaacttatag attgcaaaag tatgtttgac attctattac aagttatggg
    24241 aatagacatt tttaatgttt ccatagaaga attataaaaa tcttttatgc ttataatata
    24301 cctattgtca aatgatgaat ctcgccataa gcaaataagc ataaatataa tattactcaa
    24361 ctcaccaatt gatataccta atagagctat ataacaattc atagatttat tatttatgta
    24421 cataacaagt aaaaaaacaa ataatatttt accaatttgt tcaatgattt gtccaatagc
    24481 agggattttt acattcttta tgccatagta atatcctctt agtacgtttg aaagagttat
    24541 tataactata gcaggacaaa cagctagtat aaatagattt aatttagcat cttttaaaaa
    24601 tttaagtgat aaaaaagaac tattgaaaga tgcaactata gatataatta gtgctacgaa
    24661 aaatgatata tataaagttg atataaataa agcattagta ttgtgtctat cgtttaaagc
    24721 ttttctcttt gcaactagac aactaagtgc agtaggtata cctgttgttg taacagctaa
    24781 gcaaatcatc aaaaagttaa aaatcatatg atagatacct aaacctgttt cacctagtgc
    24841 tcttgataag aatattttat ataaaaaacc aaatattctt acaataaaat tggatataaa
    24901 taaaataact gttgatagta ctaaataagg tactttcaaa aaaatcacct ccttatcttt
    24961 taaatatatt cacaaggagg tgtaaaaatt tataatttta taaattctgg tttcaattta
    25021 ttaaatttag atacatattt aaatcccata ttttttagta atgaatatat gtagtcaaac
    25081 tcacatgcaa cttgacttat atgatgtgaa tctgaacctg tggttatgat ttcccctccc
    25141 aagtctttat acatttgaag tatatactta gatggggtta ggtctggaag attatatcta
    25201 taacaagagg tatttatctc gatacctttg ccatcataaa tagcttgttt aagagtttct
    25261 tcaattatat cagaaaatag tctatcatct aaaatagtgt cataaggtgc atacctttta
    25321 atcaaatcta aatgacctaa gacagagtaa tctttataat tcttaacaat attatataaa
    25381 taaaggtagt aattttcata aacttcatgt tgagttttat ctttaaaata attaccaagg
    25441 tataagtcca ttgtatctat agcatggata gaacatatta taaagtccaa tggatattga
    25501 tgtgcttctt ttttgtatgt atctattaat tgagtttgaa ctccaaattc aattcctttt
    25561 tttattgata ttttatcttt gtatttgctt tgaagagtct ctaaactctt aaaataatct
    25621 tcgtagacaa ttgaaaaaga atcatcagca tatacatcat agtcaacatg gtctgtgaaa
    25681 caaatttcct ccaaaccgag ttcaatagat ttttttacca tttcttccat tgtagatttt
    25741 ccgtctgtag agaaacttga gtgcatgtga tagtcgtaaa acataaatat ttcccctttt
    25801 tataaaaatt ttggtattat atatacatac taaataatat caaatataca taaaagcatc
    25861 aataaaatat aaatacaatg agcggtgata aatttggaaa aatacagaat agatttaaat
    25921 gagaaaaaat caactaaata tatacagata tttaatcata taaaagaatt gataataaat
    25981 aaagagttag tagaacatga aaaattgcct ccaataagaa aactatcaaa ttttttacaa
    26041 gtaaataaca ctactatagt taaggtatac gagcttttag aaaatgaagg atatgtatat
    26101 aaaattatag ggagtggtac ttttgtatca agtttaaatt cttataaaag cggtatagat
    26161 aataggaaaa ataagattat tgtgcaaaga gaagatttaa ttcattttga caatgggaat
    26221 ccatctaatg atatgtttcc tatcgattca tttaaaaatg ctgtaaatat ggcattatct
    26281 aaagatggta gttcaatatt tgaatatgat gaaggtcttg gctctgaaga attaagagaa
    26341 aaaatggtag agtatctaag tgatggaaat ataaaaacta ctaaggaaaa tattcaaata
    26401 atatcaggtg ctcaacaagg tatagatata gtttgtaaag gacttataag ttattcagat
    26461 gttgttttta tggaagaacc ttcttacaat ggagcaattg aagtgtttaa aagtagaggt
    26521 gcaaaaataa tatctatacc tatgttagat gatggaattg atataggtat tttaaaatta
    26581 aagttggaaa aaataaaacc aagacttata tacattatgc ctaattttca aaatccaact
    26641 ggaatatctt actcaacata taaaaaaaag aaattgatag aattggcaga agaacatgat
    26701 ttttatataa tagaagatga ttttataagt gattttgttt ttgaatcaaa ggacaataga
    26761 acaattagaa gttatgatga taaaaataga gtagtatata taaaaagctt ttcaaaaata
    26821 cttatgccag gtcttaggat aggaattgta gaaatgccaa atgagttgtt aaaaaaagtt
    26881 ttatgggcta aatattcatc agatatatca acaccaggtt taattcaaaa atcaatgtat
    26941 tactatatga ataattttga ttggaagaat tatttgagtc atatagaaaa gatatatact
    27001 gataagtata aattggcaaa aaacttgatt aatgacaaat tgagtggtaa attaaaagtt
    27061 agaaaatctt gtggaggaat aaactttttt ttagaattac caagaggata tacatctcaa
    27121 gcatttacaa attttatgtt aaacaaagga gtgtcaatgc ttccagggac ttattttttt
    27181 gacaatctag tggatgatag attctttagg ataaatatag ctcgcccaag catggaagaa
    27241 ctagaaaagg gaatatttat aataagtgat aatatagatg agtttttttg tgaatataaa
    27301 aacaagttag atataaaaag caataaatta ttttattaaa taaatagagg ttttttagga
    27361 ggaaaaaatg tttgacaaga agaaattaga taggattaat gaattagcaa agaaaaataa
    27421 agagggaatc ctttctgctg atgaaatcaa agaaagagaa attttaagga aagaatattt
    27481 agaaaacttt agagcccact ttagatcaag actagatagt gtcaaagtag tttcaccaga
    27541 agaatatgag caatacatga aaaacaataa aaattagaac ttagtaggag gaaaatatga
    27601 acttaaagta cgaatttaag aatgcttggg attttgaaag aaaagaaaat actattgaac
    27661 aaattatgca atattctagc aattatatgg agtttttaag taagtcaaag actgaaagac
    27721 tttcagtaaa agaaattata aaattggcta aagaaaataa ctatatttca atagatgaag
    27781 ctatggaaaa aggaagcata agttgtggtg ataagattta tgttataaat aaagagaagg
    27841 cagtagcact ttttgtaata ggtaaaaatt atatagaaaa aggtatgaag attataggaa
    27901 gccatataga ttcgccaaga ttagatttaa aaccaaatcc actgtatcaa gagagtaatc
    27961 taggtttttt taagactcat tattatggtg gaataaaaaa ataccaatgg acggctattc
    28021 cacttgcttt acatggtata gttatattaa atgatggaac aaaagtggac atatctattg
    28081 gggaagaaga tagtgaccca gtattttgtg taactgattt attgattcat ttggctggag
    28141 accaaatgca aaagaaacta tcagaaggaa ttagtggaga agctttaaat atattaattg
    28201 gaaatatgcc tttagaagat gaagaaaaag aacctataac agcaaatata ttaaagattt
    28261 taaatgagaa atacaatata gttgaagaag atttattaag tgctgaaatt gaagtagttc
    28321 cagctggtaa ggctagagat ttagggcttg ataggtctat ggtgttggga tatgggcatg
    28381 atgatagggt atgttcatat gctgctgtaa aagctatact agaaactgaa caaccagaat
    28441 ttacttcagt tgcattatgt gtagataaag aagaaattgg ttctaaaggg aatacaggta
    28501 tgcactcaaa gttttttgaa aatactgtgg ctgaactgat tgctttagaa ggagattatt
    28561 gtgatattaa agtaagaaga gctttagcaa atagtaaggt attatcagct gatgtttctg
    28621 ctggatatga cccaaatttt ggagaagcat atgaaaagag aaattcggca tatatgggaa
    28681 atggagtagt tttaactaag tatacaggtt caagaggaaa gagtggatgt aatgatgcta
    28741 atgctgaatt tatgtcagaa gtaaagagaa tttttaataa aggaaatgta gtatggcaaa
    28801 ctgctgaact tggaaaagta gaccaaggag gaggcggaac tatagcccat atattggcaa
    28861 atcaaggtgc agaggttata gactgtggtg ttggagtttt aaatatgcat gcaccacatg
    28921 agattgtctc taaagtagac atatatgaaa tgtataaggg atataaggca ttttttaata
    28981 taaatttata agtaaaaata taaatgtaat aagtgaataa aggctgttgt ataaacaatt
    29041 taatctgttg tacaacagtc ttatttttat gtaaaaatag caatttgtta cactagagaa
    29101 aataaaagaa aatactgtca aattaaaaat tatttaaaaa aaaattgaca acgatttcat
    29161 cttgttataa tatgtaataa agaacttgtt atataatgtt ataaaaatgt tacataggga
    29221 ggtgagcaaa atggatgaaa agagacaaaa aaaagaaatg gatttagaag taaattcctt
    29281 tgttccttta tatcaacaac tatatgacaa cataaaaaaa caaatagcat ctggcatata
    29341 taaaccagga gataaacttc catctgaagg agacttatgt aaagaattta atataagtcg
    29401 tataactgtg agaaatgcac ttaatgaact tgtaaaagaa gatatattat gtaaaaaacg
    29461 tggtaaaggg acttatgtaa caataccaga gagaatagaa gcaacatgtg caggaaatag
    29521 ttttactaat tcttgtcatc gtatcaatgc aaaaccaagt accaaaataa tctctgtttt
    29581 aataaaaaaa gcagataagc aagtagctga agctttaagt attgaactag aagagaaggt
    29641 aatatgtatt aaaagactta gattaataga tgaagtacct gttatttttg aagttgacta
    29701 ttttagaata gattacatgt ttttattaaa agaagaatta gaagataaat ctttaatgga
    29761 ggttatatct aataatataa gcacattacc taaacgagta gaaaatatat ttgaagtaaa
    29821 acattcaaac aaagaatatt ctgaccatct taaatgtgca agtaatatgc cactattgaa
    29881 agtaagacaa tcggtgtata cagaaaataa tgatgtctta tattacaatg agcaatttat
    29941 aagaagtgat aagtacaaat atgcagtatc agcagaaata taatctataa tttagtagga
    30001 ggatgagtat gaaaaagata atattagcat ctcatggaga cttttcaaaa gggcttttaa
    30061 atgcagtaaa aatgatagta ggagatttag cagattgtgt cagttcgtat ggtttgtacc
    30121 ctggacaaag tgcttcagat tttgctttag aattagaaaa aacaatatca gaagataaag
    30181 aatgtgaata tataatttta tctgacttat atggagcaag tgtatgtaca gcaatgttaa
    30241 gattaactaa tctacataat gttaggttat tttctggaat gaatttgaat atggtattag
    30301 aacttcttac aaggttttct gatgaattaa cagttgaaga tatagaccaa ttagtggaag
    30361 aatctagaag aggaatacaa agcgtgacat tacaaattaa agaagaagaa gaggtatttt
    30421 agtttatgaa aaaaaaggga atattatcag tatttaaaag taaaaaacca attatagcaa
    30481 tgatacactt aaaaggagat actccagaag atatctttga aagagctaaa aaagagatta
    30541 caatctttga agaaaatggt gtagatggaa taatgttaga aaattactat ggtaattatt
    30601 atgatttaga aagaatactt gaatatgtat caaaagctaa tttatcaata ccatatggag
    30661 taaactgttt aaatgttgat actatgggat ttgaactggc aacaaaatat aatgctagtt
    30721 atatacaggt tgattctgta gtaggtcatg ttaaacctag agatgaagct actttagaag
    30781 agttttttaa attacaacgt tcaaaatgcc cagcgtattt aattggaggc gtacgtttta
    30841 aatatcagcc agtattatca gaaaatgatg ttgaagaaga tttaaaaata ggaatgacaa
    30901 gatgtgatgc tatagcagtt acagaaaatg ctacaggtca agaaacatca atggaaaaaa
    30961 tagaactgtt tcgtaaaaat ttaggagact tcccattggt tatagctgct ggagttacat
    31021 tagaaaatgc aaaaaaacag cttaaattag gagatatggc aataatagga agttacttta
    31081 aagataatta caaagatttt ggggatgtta gtgtagaaca cgtaaaaaca ttcatggatg
    31141 aaatcaaaaa aattagggag gaattataat gattaaatta gtaagagtag accacagact
    31201 tatacatgga caagtagcat ttacatggac aaagttttta agcacagact gtatattgat
    31261 agcaagtgat gacttattaa aagatgaatt gagaatggca ggacttaaaa tggctaaacc
    31321 atctaatgtt aagttagtaa tgaaaagcat agcagattct ataaaagcac ttaactcagg
    31381 tgttactgat aaatacaact tattgatact ttgtgaatct gtagaagatg tttatagact
    31441 tgctaaagag gtaaaagcta ttaaatctat aaaccttggt ggaacaaaat cagatgataa
    31501 tcgtgaaaat atatctaaag cagtgcatgt atcaaaagat gatataaaga tgattaaaga
    31561 gttagattca gaaggcgtaa atgtatttgt acaattagtt cctgatgatg atgctacaaa
    31621 tgtaatgaaa ttaatataat tttaggggga tgaaaatgga atttacacaa gttattttga
    31681 taactctaat tgcattcttt gcatatatgc atagttttgt aggttctaca atgcataata
    31741 gacctatagt tgttgcacca cttgttggtt tagcactagg taatcttcat acaggtatag
    31801 taataggttc aacattagaa ttagtattta tgggtgcatt tcctgttggg gcaagtaatc
    31861 ctcctgattt tgtgtctggt tctattattg ccacagcgtt tgtaatatta acgggtcaag
    31921 atgtatcagc agcagttgta cttgcagttc ctatagcaac tctagtattg cttatagata
    31981 acttcttaat gacagtagta cttacatggg gagctcatat tgctgaccgt tatgcagaag
    32041 aggggaatat tgaaggcgta gaacgtgtgc aattattatt tggtatagga aataaattaa
    32101 tattagcaat aatagtaggt ataggatttt cattaggtgt tccagtaata gaaaaaatac
    32161 tttcatttat accatcatat gtaactcatg gaatggatgt tgcagcaggc gtgattccag
    32221 caataggttt tgccatgtta gctagaatga tgcttaataa gaaaacagta gcatttttat
    32281 tattaggatt tatattggtt gcttacctaa acataacagt aacaggagtt gcattgtttg
    32341 gattagcaat agctttaata tatgttaact ttgtaggaga gaaggaggta atagtagatg
    32401 acaacgagtt ctaagaaatt agagacaatt tcaccagata gtaaaataac gaggaaagac
    32461 ttttggaaat gttttagaag gtcattgaca ttagattcat cttggaacta cgaacgtatg
    32521 caaaatatag catatgcata catgatggca ccaataatcc gtagattata taaagatgat
    32581 aaagaaaaga aatctaaggc tttaaaaaga catttagaat ttatgtcagt tactcctcat
    32641 ataagtactc ttttggtagg tatatctgga gctatggaag aagaaaatgc aaagaataaa
    32701 gaatttgatg ctaatagtat aaatgctgtt aaatcaagtt taatgggtcc agtttctggg
    32761 ataggagatt catttttctg gggaacatta aagcttatag cagctggggt tggtatagct
    32821 ttagcttctc agggtaacat aatgggaccg attttattcc ttttaataat aaatgttcct
    32881 cattttatta tacgctatat atgtctagat aaaggattta agtatggaac tcaattcttt
    32941 aaagatgtga gtggttcaag tatagtttca aaagttatgg aagcagcttc aatgttaggg
    33001 cttatggtaa ttggaggtat gactgcatct aatgtaatgt taaaacttag tgttaatgta
    33061 ggtagtggag aatgggcaga gcctattcaa acttacttag accaaataat gccatgtatg
    33121 cttccagcta tgatattcgg tattatgtat tggttattag gaaaaaaggt aaagacaact
    33181 acgatattaa tttcagttat gattatttgt atagtattgg ctgctattgg agtagtataa
    33241 ataaaaaaat cccaagttca tttgaacgag ggattttttg actaaattaa ataaaaaaga
    33301 aagatggttc ataataattt ataagcattt atgtgatagt tataattagc ttattaatta
    33361 gttataaact caaaattaag cactacttta ttaaagttat tatattataa tttcgaaata
    33421 ctcacataat ctgtttagaa atttatcttc taatggggaa aaagttttct ttttagagta
    33481 aatgaagaaa aactttctag taaaatcaac atcctttatt ttataacatt ttattttacc
    33541 tgcattgaga taatcaatag ctgaaatgta cgatataaag gatactccaa gacctaatct
    33601 aaccatttcc ttaatagcct catttgattc tacatgagcg attacattta atttacttac
    33661 tggaaagtta tttttactaa gtgtgtttag tattaagttt cttgtacctg acccttcttt
    33721 tctcataata aaatttagat aagccaattc tcctatatcg atacaattat ttttattatc
    33781 aattttaaaa tctgaaggag tgataagaac aagttcatca tctaataagt ctaggtactc
    33841 tatttgagga ttattgattt tagaaccaac taatccaaag cttattcgtt catttaaaat
    33901 ttctgatata gcatattgag aatcataatg gcttatggaa aattttacat ctggataact
    33961 catagaaaaa ctcttcataa aatcaggtaa aatataagtt tcaggtatgg aactacacgc
    34021 tatatctata ataccttcta tttttcctga atattctttt atatcataaa ttgcacgttt
    34081 gcaattattc aatatatata tagcatgttc gtaaagaatt tcaccagatt ttgtgagtgt
    34141 aataactttg ttgcttctat ttataagaac agtttctagc tctctctcta aattctgtat
    34201 gtgtgcgctt acagtaggtt gagttaaaaa aagttctctt gcagcctttg aaaaactctg
    34261 atgttttgca acagcaacaa atacttcaag ttgtttaaaa tccattattt aatcctcctt
    34321 aaattatcta aaactattat aacatatttg ataaattaat agtcaaaggt gatttttaaa
    34381 atagaaaacc tttaaatatt catggagttt ttgattgttt aggaataatt tggtataatg
    34441 ttgtaaggta cttacaataa aaatatcgta aattgaattt tcaaaattca actttaaagt
    34501 gttttttatt acagaaaa
//
LOCUS       DAEMHZ010000032        33790 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000032 DAEMHZ010000000
VERSION     DAEMHZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.032"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(263..1738)
                     /locus_tag="KPK72_001394"
     CDS             complement(263..1738)
                     /locus_tag="KPK72_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HBG2488625.1"
                     /translation="MNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAINYMK
                     KYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLIEDCELIISNLNYLSNIIENSLN
                     KKTKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYTYEG
                     NDFPEDVPVIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNMLVY
                     VAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQILTMGSPKF
                     DSLIYNLKQKNINKNYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIENSQE
                     YGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSHALI
                     TDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYVVGEEDMDLIKFMNLVKN
                     NNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
     gene            complement(1776..2516)
                     /locus_tag="KPK72_001395"
     CDS             complement(1776..2516)
                     /locus_tag="KPK72_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2488626.1"
                     /translation="MGNATRIYEKHVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQVCDFINTLYDTKVKSKMPFDTILLAGVTMYINDDELKIALKN
                     LLGILDKKATIYISIPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLDEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            complement(2529..3173)
                     /locus_tag="KPK72_001396"
     CDS             complement(2529..3173)
                     /locus_tag="KPK72_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBG2488627.1"
                     /translation="MPTTKTIVVNCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINGGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            complement(3146..3763)
                     /locus_tag="KPK72_001397"
     CDS             complement(3146..3763)
                     /locus_tag="KPK72_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HBG2488628.1"
                     /translation="MKGNEMENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNE
                     TMLGNLSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNL
                     DIWICDLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSND
                     IKMIENADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
     gene            complement(4465..6606)
                     /locus_tag="KPK72_001398"
     CDS             complement(4465..6606)
                     /locus_tag="KPK72_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2488629.1"
                     /translation="MLLSIVMMVKNEENILEKTLKSLSVLRNSIESELIIVDTGSTDN
                     TIRIAKQYTEKVYFHNWNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFES
                     GLNKKFNSASVRLKNLYSFDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNI
                     ADFEHYGYLFEDEEIRINKVKRNEKLLLEELKEDEKSPYTNYQIGKNLIILGKYQEAL
                     DYLEKSYKLYSKLEIVPGYLITNLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYY
                     KLEKYDLVISEYDNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYREIPNSKVERDSF
                     YKSMEDFINNIKDDEKELIYKSLCDIEGNYGLLNKIRINKHVSLDTCRKILNEEKDIV
                     YASVIKIAFEEKVDLLDIFYNMEYVWIEKYLRYILALERSFNVSIYKYIISCPNTSEI
                     EKIRVYKVISKILLESVNLSDKKYKELFYIYIMYSYKYIKYIYSNLSDCELIKFVCND
                     IDKFTLEFKHVLDGKRKINNPNEKLEYLHNMRILLNEYPFYNRIIKIFISELEESITE
                     SEEFKLLKDNFIIRIENMIENGKINDAKLLIEDYSKTFADDVKILNIKAILFMCEDKF
                     EEADFALKKALSLDLENEDTIYNIEYLKNLK"
     gene            complement(6700..7572)
                     /gene="fliC"
                     /locus_tag="KPK72_001399"
     CDS             complement(6700..7572)
                     /gene="fliC"
                     /locus_tag="KPK72_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBG2488630.1"
                     /translation="MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEIN
                     NTEEREKIADELTQLKDEIERISSSTEFNGKKLLDGTSSTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTASIGSMKAGGTTGTDAAKTMVSSLDAALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(7673..8008)
                     /locus_tag="KPK72_001400"
     CDS             complement(7673..8008)
                     /locus_tag="KPK72_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG2488631.1"
                     /translation="MDKLEEKINLYKDISLKIINFIEKMEYKNISFQLDERQNIINSI
                     SEVDKSEFIQLYDSMELFEIDAKIRDALQEQLSEVKKELHEYKLTKQVNTMYYSSNRE
                     KVNIFNKKV"
     gene            complement(8011..9534)
                     /gene="fliD"
                     /locus_tag="KPK72_001401"
     CDS             complement(8011..9534)
                     /gene="fliD"
                     /locus_tag="KPK72_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG2488632.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSDIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKDDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKALAGYSSNYDDKGMFEKLKKIVFEYSGSSASKLTKKAGMENSSSA
                     SQNVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNKYSSQMNYFSQAQGN"
     gene            complement(9562..9912)
                     /gene="fliS"
                     /locus_tag="KPK72_001402"
     CDS             complement(9562..9912)
                     /gene="fliS"
                     /locus_tag="KPK72_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2488633.1"
                     /translation="MELDLNELNGLSKEELLLMLVDGTVKYTNISKVALLNKDYLKAH
                     NELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPIV
                     EQIRDTWHEVYNKL"
     gene            complement(9912..10307)
                     /gene="fliS"
                     /locus_tag="KPK72_001403"
     CDS             complement(9912..10307)
                     /gene="fliS"
                     /locus_tag="KPK72_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2488634.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDVQII
                     DNVLPVIEEIKDTWHEADRKSREERSRYK"
     gene            complement(10329..10541)
                     /gene="csrA"
                     /locus_tag="KPK72_001404"
     CDS             complement(10329..10541)
                     /gene="csrA"
                     /locus_tag="KPK72_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG2488635.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            complement(10535..10927)
                     /locus_tag="KPK72_001405"
     CDS             complement(10535..10927)
                     /locus_tag="KPK72_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG2488636.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDILKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCRMQIILQDEEYSIWHPLLRGDGGC"
     gene            complement(10943..11875)
                     /gene="flgL"
                     /locus_tag="KPK72_001406"
     CDS             complement(10943..11875)
                     /gene="flgL"
                     /locus_tag="KPK72_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG2488637.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     TSLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(11894..13204)
                     /gene="flgK"
                     /locus_tag="KPK72_001407"
     CDS             complement(11894..13204)
                     /gene="flgK"
                     /locus_tag="KPK72_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG2488638.1"
                     /translation="MAGLFSILNTANSGMNAHQKSIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNSSAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(13222..13638)
                     /locus_tag="KPK72_001408"
     CDS             complement(13222..13638)
                     /locus_tag="KPK72_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG2488639.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQLLGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(13643..13918)
                     /gene="flgM"
                     /locus_tag="KPK72_001409"
     CDS             complement(13643..13918)
                     /gene="flgM"
                     /locus_tag="KPK72_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG2488640.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEAARNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            complement(14069..14383)
                     /locus_tag="KPK72_001410"
     CDS             complement(14069..14383)
                     /locus_tag="KPK72_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2488641.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIEENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     VN"
     gene            complement(14407..14835)
                     /locus_tag="KPK72_001411"
     CDS             complement(14407..14835)
                     /locus_tag="KPK72_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG2488642.1"
                     /translation="MGINIDKLNISPSRNIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTSKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(14844..15512)
                     /locus_tag="KPK72_001412"
     CDS             complement(14844..15512)
                     /locus_tag="KPK72_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBG2488643.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKSRIENAVEKA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(15958..21039)
                     /locus_tag="KPK72_001413"
                     /pseudo
     CDS             complement(15958..21039)
                     /locus_tag="KPK72_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(21050..22582)
                     /gene="purH"
                     /locus_tag="KPK72_001414"
     CDS             complement(21050..22582)
                     /gene="purH"
                     /locus_tag="KPK72_001414"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG2488644.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLGDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(22575..23168)
                     /locus_tag="KPK72_001415"
     CDS             complement(22575..23168)
                     /locus_tag="KPK72_001415"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG2488645.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGVKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(23162..24238)
                     /locus_tag="KPK72_001416"
     CDS             complement(23162..24238)
                     /locus_tag="KPK72_001416"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG2488646.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(24232..25599)
                     /gene="purF"
                     /locus_tag="KPK72_001417"
     CDS             complement(24232..25599)
                     /gene="purF"
                     /locus_tag="KPK72_001417"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG2488647.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(25611..26309)
                     /locus_tag="KPK72_001418"
     CDS             complement(25611..26309)
                     /locus_tag="KPK72_001418"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2488648.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(26315..26791)
                     /gene="purE"
                     /locus_tag="KPK72_001419"
     CDS             complement(26315..26791)
                     /gene="purE"
                     /locus_tag="KPK72_001419"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG2488649.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            27241..27753
                     /locus_tag="KPK72_001420"
     CDS             27241..27753
                     /locus_tag="KPK72_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2488650.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            complement(28137..29300)
                     /locus_tag="KPK72_001421"
     CDS             complement(28137..29300)
                     /locus_tag="KPK72_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2488651.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(29297..29695)
                     /gene="tnpA"
                     /locus_tag="KPK72_001422"
     CDS             complement(29297..29695)
                     /gene="tnpA"
                     /locus_tag="KPK72_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG2488652.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            30143..30358
                     /locus_tag="KPK72_001423"
     CDS             30143..30358
                     /locus_tag="KPK72_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488653.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            30364..30657
                     /locus_tag="KPK72_001424"
     CDS             30364..30657
                     /locus_tag="KPK72_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG2488654.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(30881..32944)
                     /locus_tag="KPK72_001425"
     CDS             complement(30881..32944)
                     /locus_tag="KPK72_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBG2488655.1"
                     /translation="MGILDEIMNQERFDIKTSLDYINEYEKLLPFDSEIHTARAVTYF
                     FGEEYKLAEEHIRKALKFRPNNYDNNFYLANILIAMGEYPDAIKSAVLSVTFLKHFAK
                     IQGYDYINDKLFYYNEQVKTLIEYCIENIKDTNVLEDLKRFFGFIELSIAKFPVCFYG
                     DKWNILVGEYLNKDLNSEYNDYICFYSDTYNNMLSDFQKKLYTVINNSEISSICPIES
                     FKSMTAKEFSLDNGKYIIPIATTEYNQVIDFKIKNTNFKSQQVDPPGFFKYYRVNNGI
                     SLNSDKDVIIGKPIKLGFKEKNKKLVFTIFIDALSQKYLLETKYKSMPYTKAFFDKGI
                     IFENCYSAGEWTYPSLTSVVTGLYTTNHHFLHSSAGYKYPDDIDLFSEIMKKNEYFTA
                     NISGTYALAPYSSGFRGFDRTVFKNSLGFSDSVIINDAIKHIESFKETNQFVFMSLSD
                     VHKILDDIENYSVNYDIVNQTSLDIETSLSKRSSFVSAWQKYDEEAIKKYDVALRELD
                     RQLKGIYDYILQNYSEEEYLISIFSDHGASFLGKDDFLLKSNITNSVLMVRGSGSASN
                     GNTEYISNMDLIPIILKLANIEHDLSKYDCVLPKTLGGKGREFTYSESIYPGQTYKAM
                     VNNDKYCYILETKGITDIDGTINFDEYSDSVFDKKEDKDVKDNDILEYYREIVYNHIK
                     RNIKY"
     gene            complement(32962..33687)
                     /locus_tag="KPK72_001426"
     CDS             complement(32962..33687)
                     /locus_tag="KPK72_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP--phosphocholine cytidylyltransferase"
                     /protein_id="HBG2488656.1"
                     /translation="MKAIILAAGLGTRLKPLTSECPKPLVKVNGKPMIETQIECLLEK
                     GIKDIKVAVGYLKEKFYYLAEKYNVELIYNNKYDIYNNIYTMYLLREHLPDSYVIEGD
                     VYICNNFIDPDIKESTYFTLYKENFNNEFIFKFDENNKVYDMYVGDNKGYILCGVSYW
                     SLTDGNIIAKKLEETIENGDFESLFWDDVVMKNKDALNMYIQKIDFKDCFEIDNEKDL
                     RFSEKCIVQNNKFTMKKILQYSK"
BASE COUNT        10531 a         5926 c         3480 g        13853 t
ORIGIN      
        1 ggattttata tctatttaaa gttttccctt ttattttcac ttttagggat aaaaaatttg
       61 taattttctc tacttttgat aaattttatt ttagactggt tatataagaa tacagtctaa
      121 aatgttgtat agagtaacaa aataaattta aaaaaggtca aactttaatt aatttgccct
      181 ttattatctt atatttatta aaattgataa tgttttaact cctttccaag cctacattta
      241 tctaattatt aattaatttt aactaaatat ggttatttaa aacgtattct aatacattag
      301 tatgtatttt ttcaccacaa cttccatctg tatttgatag tacactattt aaagaattta
      361 atctttgatt tagcttataa tcattattat tttttactaa gttcataaat tttattaaat
      421 ccatatcttc ttctcctact acataatttg aaaatatatc gaatattctt gaatcataat
      481 atccacgttc catatctata tcataaatta atataggttt cttcgtttga atatattcta
      541 ttaacacaga agacctatct gttattaaag catgggataa gttgaatgat tcatagtaag
      601 atacagagtc gtctaaaata atattatttt ttatagatat tgaatctagc aaagttttgt
      661 atctattaaa acattctgga actttagatt ttacatactc aagttctaat ggatgtggtc
      721 tatatataag accatactct tgactatttt caataatatc aaatacattt tcaatctcat
      781 caatgacacc atctttgcca ttaggtattt tcattagatt agttgtccat aagaaaatta
      841 ttttaccctt taatttagat tcataatttt tatttatatt cttctgtttt aaattataaa
      901 ttaaagaatc aaattttgga gatcccattg ttagtatttg atttttattt ataccactac
      961 attctgctat aatttcaaat gcaggagatt gcacaataac cttatcaaaa tttctagatg
     1021 ctatcaattc ataaaaattt aagtaaaaac taacaggata agttccaagt attccataag
     1081 caacatatac aagcatatta gtatattgtt tcaagttata agaaaaataa ttactatcta
     1141 ccattgtagc attgttacaa tcatcatatt gattatgaac atatattata tcaggtttct
     1201 ccttagataa atcaaaatca tcataatgta ttactggaac atcttcagga aaatcattac
     1261 cttcatatgt atatatctga gttataccat taggcgtatc aattaacttg taataaggta
     1321 tgggaaccac ataacagtca cactgctcat ccaaaaccgc agatttccag atactttcta
     1381 aactattcca cattttagca ttataaggca taaatacaat tttagttttt ttatttagac
     1441 tattttctat tatatttgat aaatagttta aattacttat gataagttca caatcttcaa
     1501 taagtcttat atttttatac atggaaagtc ttaaaatttt tacataagtt tcatcaatta
     1561 aattcaacaa gtttttgttg cttcttccag aattatttat atattttttc atataattaa
     1621 tagcttcaag agatgaatta actattgatt cacttaaatt attatttttt atgtaatcaa
     1681 tgccctctat aagtatttgt ataagattta aaacttcatt ttttttatta tagttcatca
     1741 gattcacctt aattttgatt ttatttatat taaaactatc gctttagtat aaaataatgc
     1801 ctttctgtat cagagtattg atttatatct gttaagaagc ttttgctttc aacaatttca
     1861 aatccttcat ctagtaatgg ttttaaaatt tcttcatatt cttctatagt tctatatata
     1921 acattatatt ctgaatttaa ttctttcgag taaaattctt tcaaagttaa tctcttattt
     1981 attccaatag gtatagatat atatattgtt gcctttttat ctaatattcc taataaattt
     2041 ttcaatgcta tttttaattc atcatcattt atatacattg ttacacctgc caatagaatt
     2101 gtatcaaatg gcattttgga tttaactttt gtatcgtata gagtattgat aaaatcacat
     2161 acttgaaaat cacagtcatt atcagaaaat ctttttttag caatagatat taagtcttca
     2221 gcaaaatctg tcccaagata atagttacat ataggaacta tatcatcagc taacctacct
     2281 accccacaac ctatatccaa tatatttgaa tttctagtta tatttagttt aggaattatt
     2341 atttcttttt catatttatc taaattttta gcataatcag gattttggtc attacatttc
     2401 actgaaatat agggattctc tggaatatgc tttttagctc gtttattcca aaaatcttga
     2461 acagtatctt tttttatatc tacatgtttt tcataaattc tcgttgcatt acccatataa
     2521 aatctccttt attttttgtt cctataccat tttaaagctt cattatattc ttcaggtgta
     2581 tcaatttccc tcaattcaac atgagctatt ttaagtggta agttgggtga aatcattttg
     2641 tatacatgat ttgtaccaga gcttaaattt ttagaatata ttttaaacaa acctgtccac
     2701 tcataatctc catttgtcct tgaaaattct gtagcaaact ctccaccatt aataaagtct
     2761 gttttaacat agacagggtc tttagtataa ggttgacata caccaataat ttctccatct
     2821 gtgttaacaa aattatcgaa atcttctttg aaaaaaagta tatcaccatc tagtgatata
     2881 acagtatctc tagaatattt tttgcctaaa gataagcttg cagcagtctt agtgtttaag
     2941 taatcatgat taaatacaaa cattatatct ttacgataag aacatacaac ttcaattaat
     3001 ttttctgctt gaaagcctac tacaatacgt atgtcatcat aatcatcaag acactctaat
     3061 tgtcttatta ttaatggctt tccatctata tctattaacg acttatttga atttaatcct
     3121 agtctacttc ctctacctgc acagtttaca acaattgttt tagtagttgg cataaacgtt
     3181 cctcctcata aaatgcataa tcacatactt ttaatattcc aggagctata ggtcttaccc
     3241 ctccaaatcc aattccaata tcagcattct ctatcatttt tatatcatta ctgccatcac
     3301 caactgcaac tataggacct ttcatttttt ttataatatc ttcttttttt attataactt
     3361 taattttatc aatgaaatca ccatttgaat gagctataga tgaatagtat ttattttcta
     3421 aagaaagctc tttcattaaa tcacaaatcc aaatatctaa attatttgtc acaacagtac
     3481 atctatctgg attttctttt ataaacttta caattttttc attaagatta atcttagcaa
     3541 ctatactttt aactttagaa ataggaatac attttaataa atcgactctt ttcttaaaac
     3601 tttcctcaaa agataaattt cctagcatag tttcatttgt aagattttgc ataagttcat
     3661 atttatttat acacttagaa atctcaggaa gtatttcttg tttagttaat gttgaatcta
     3721 aatcaaaaac aaatttataa ttttccattt cattcccttt cattgaagca taaaatccta
     3781 tttttgagtg ataagtacat caaacaatat tgagttagaa atttcaataa ttttacagag
     3841 tttataattt tataatataa ttttattctt tattataaaa ttgtatatag ctcaatcaaa
     3901 aagctaaaaa ctaaatctta tcaaaataaa gaaatcttgc gttcttaact aacaaaaata
     3961 ctctataaat ggtttttaag tattaaaatg acttaataaa aattcaatta ttacaaatac
     4021 tgctgtttta tgtcgaatta attcaattat ttaaaaacac taaaatcatt gaaattacta
     4081 aaaaattgtc ttaatgcgca aaaccttcgt ttttaggata ttgcccgatt taattatata
     4141 catattatcg tctaatttta aaaaaaatta agagaaaata tgtataattt tcatttatat
     4201 tgttttttgt tgtttatttt ttgaacatgc ttgtctattt tattattata agtaaatatg
     4261 ataaaattag tttcccttga attaaatctt ctatatacta aaatcattga gtatattcta
     4321 ttttatttga tgccaatatc gatatatatt gctgtaccag taaattttac atcagtattt
     4381 ttatatgtat atattcaatc ttctattata tttttcatta actgttattg atattattat
     4441 tttctaaata acctagtttt aaatttattt taaatttttt aagtactcta tattatatat
     4501 agtatcctca ttttctaagt ctagtgataa tgcctttttt aatgcaaaat cagcttcttc
     4561 aaatttatct tcacacataa acaaaattgc ctttatattt aaaatcttta catcatctgc
     4621 aaatgttttt gagtagtctt caatcaataa ctttgcatca tttatttttc cattctcaat
     4681 catattttct atacgaataa taaagttatc ttttaataat ttaaattctt cactttctgt
     4741 tatactttct tctaattcac ttatgaagat ttttattatt ctattgtaaa aagggtattc
     4801 atttaataat attctcatat tatgtagata ttctaatttt tcattaggat tatttatctt
     4861 ccttttacca tcaagtacat gcttaaattc taaagtgaac ttatctatgt cattacaaac
     4921 aaactttatt aattcacaat cactcaaatt gctatatatg tattttatgt atttataaga
     4981 gtacattata taaatataaa aaagttcttt gtatttttta tcacttaaat taacactttc
     5041 taaaagaatt ttagatataa ctttgtacac tcttatcttt tctatttctg atgtattagg
     5101 acaactaatt atatatttat atattgatac attaaaactt ctttctaaag ctaaaatata
     5161 ccttaagtat ttctctatcc aaacatattc catattgtaa aaaatgtcta ataagtcaac
     5221 tttttcctca aaagctattt ttataacact tgcataaact atgtcttttt cttcatttag
     5281 tatttttcta catgtatcta atgatacatg tttgtttatt cttattttat tcaaaagacc
     5341 ataattacct tcaatatcac aaaggctttt atatatgagc tctttctcat catcttttat
     5401 attatttata aagtcttcca tacttttata aaaactatct ctctcaacct tcgaatttgg
     5461 aatctctcta taatagtttt ttatgtcttc aaatctattt aatttatata aagacataaa
     5521 taagctaaag tacacatttt tctttttttc cacatcttca atattatcat actctgaaat
     5581 tactaaatca tatttctcaa gcttataata aatttttatc aatgtaatta tagcttcatc
     5641 tctaaatcct acagcatcag tatctgaaag caaactattt gcttgcgtac tcatttcata
     5701 attatctagc aaataaatat atcttttata tgaatctata ctattttcat acttccctaa
     5761 atcaacttgt gcttgtgcaa tatgataata tatatcaata ttatttctgt ccttattaat
     5821 atattttaag cataattttt cacacttctt agcctttcct aagtataaat atacttttgc
     5881 aaggtttgtt atcaagtatc caggtactat ttctaatttt gagtataatt tataagactt
     5941 ttctagataa tctaaagctt cttgatattt tcctaatata ataagatttt tccctatttg
     6001 ataatttgta tatggagatt tctcgtcttc ttttaattcc tcaagcaaca gtttttcatt
     6061 tctttttact ttatttattc ttatctcttc atcttcaaac aagtatccat aatgttcaaa
     6121 atcagcaata ttattaaata taggattttt atacattggt tgttcatgaa ctttcccaca
     6181 gtatttaaaa tcaaggtttt tgaaaaatct caacactgaa caatatccat ataatttttt
     6241 atcaaatgaa taaagatttt ttagtcgtac tgaggcagag ttaaattttt tattaagacc
     6301 actttcaaaa aactttataa tatttgaata atcaattaat acttcatctg catctaatat
     6361 aagtaaccat tcgcccttag cgtaggatat agatatattt ctcatactag aaaaatcatc
     6421 attccaatta tgaaaatata ccttttctgt atattgtttt gctattctta tagtattatc
     6481 tgtagaacct gtatcaacta ttataagttc actctctatt gaatttctca atactgataa
     6541 cgattttaaa gttttttcaa gtatattttc ttcgtttttg accatcataa ctatactaag
     6601 taacatatat attttccttt caaaactata tttctaaaaa aagaaaagaa taaagcaaag
     6661 ccttatcctt tcttttttat tcaatatact aaatttattc tatcctaata attgtaaaac
     6721 tccttgtggt tgttgattag cttgagcaag cattgattgt gatgcttgaa ctaatatatt
     6781 cattttagat aagttaacca tttctgaagc aacatctgta tctcttattc ttgattctgc
     6841 tgctgtaaca ttctctaaag tattatttaa attgttttga gttgattcta gtctgttttg
     6901 ttgtgctcct aattttgctc ttgatgagtt taagctctta agagctgcat ctaagctaga
     6961 taccatagtt ttagctgcat ctgttcctgt agttccacct gccttcatac ttcctattga
     7021 agcagttgtt ataccagctg aagccattat acttgcagta tttactaatt gaatttttat
     7081 ctcattatta ttattacttg tacctgaaac attagttcca taacttgctc caacttgtaa
     7141 tcttattgtt gaagaagttc catcaagtag cttcttaccg ttaaactctg ttgaacttga
     7201 tattctttca atctcatctt ttaattgtgt taactcatca gctatctttt ctctttcttc
     7261 agtattatta atttcatttg cagattgtaa tgataaagtt ctcattcttt gtagtatatt
     7321 tccagtttct tctaacgaac cttctgctgt ttgtacaaca gatataccat cttgaacgtt
     7381 tcttcctgct tggtctaaac cttttatttg agctctcatt ttctcagata ttgcaagtcc
     7441 agcagcatca tcagcagctc tcttgattct aaccccagaa gataacttct ccatagactt
     7501 actttgtcca tttacgtttc ttcccatttg attgtttgct atcaaagcac ttacatttgt
     7561 attaactctc attattttac cctccttagt atagttgaca tccatgtcat ttctactttt
     7621 aacttttgta ttaactatcc gtttatatta tcggacaagt taatctataa ctttatactt
     7681 ttttattaaa aatatttact ttttctctat ttgaactata atacattgtg tttacctgtt
     7741 tagtaagctt gtactcatgt aattcttttt ttacttctga taattgttct tgcaaagcat
     7801 ctcttatctt agcatcaatt tcaaatagct ccatactatc ataaagttgt ataaattcac
     7861 ttttatcaac ttcacttata ctatttataa tgttttgtct ctcatctaat tgaaaactta
     7921 tatttttata ctccattttt tcaataaaat ttataatttt taaagatata tctttatata
     7981 aatttatttt ttcttctaac ttgtccataa ttaattacct tgtgcttgtg agaaatagtt
     8041 catttgtgaa gaatatttat tcaacaaaga ctctaaagct gaatatttag cataaagtct
     8101 tttctcttta tcattcattt tttcaactaa cctacttata tttctttctt gctctgcaat
     8161 ttgttttgaa tatacatttt gactagcaga cgaagaattt tccatacctg cttttttagt
     8221 aagtttagaa gctgaacttc cagaatattc aaatacaatt ttttttaatt tttcaaacat
     8281 acccttatca tcataatttg aagaatatcc agctaaagct ttatatacct tgtcactatc
     8341 atctataaga gccttcgaaa aagtatctgc attaaatgat atttgtcctt ttttgttata
     8401 attctcatgc tcactaatac ccagttttct taaagcaata atagtatctg catctccaaa
     8461 aaacatagac tgaatatctt caacaaaagc tcttaattca tcatcatttc taagtattcc
     8521 ttcctttgcc ttcttttccc acttttctat ttcttcagtt gtcatatcat ctttttgttc
     8581 atctgtaaga ggtggataat cttttgattt tttagtagta actaatccat aaaccttatc
     8641 catcaatgca ttataatcat ctacaaatgc tttcatattt ttagtagctt cttcagtgtc
     8701 agtaacagaa accagtttag cacttccttt tgaatttaca ttataatcaa tgttatctat
     8761 tttaaatgaa ttctgttctt tacttatgtg tgttactctt ccttggtcat cagttacatc
     8821 agcttctaaa ttttttccat atactactgc agatttagtt ccactagcat cagttgtaaa
     8881 tgtaatacca ttatcactgg caatttgtcc agctaaacta tcatttccaa ttacttttaa
     8941 atcaatatct gatgatgaac cagtctgttt tcttgagata atcaattcac ctgtcatctc
     9001 actataagaa gctcttatat cattatctgc acaaagggag tttatcttac ttacaatagt
     9061 acttgtagta tcttgttcag atattggtat atttacacca cctatattta aagaacttgc
     9121 tccactagca tttggaggaa actgttgaac aatagggtca cttgaattta acttaatagt
     9181 tacttttgct ggttcagcca tttgagaaac tgcaaattga taatttattt tttctgcacc
     9241 agctgagcct tttgctacta taatactttc atcagaacta gttattcttg tagcagaata
     9301 tgctttttta tttgttatac tattaggaga gtctccattt aaatacttgt cataaagatt
     9361 ctttgactct tttataatat ccctgtatat ttcttgtttc catttaacta tttgttgatc
     9421 ttgtttcgct ttattaactt tttccttatc tcttatcata ctagcttcta ttataccttc
     9481 catatcaaaa tttcctgaaa ggcctgtaac tcttactgga cttatacttg acatatgttt
     9541 atccccccat catgattata gttatagttt attatacact tcatgccatg tatctcttat
     9601 ttgttctact attggcaata tatcatcaat tattttaata tctttattta tatcagcctt
     9661 tactaattca tgttttatga attcataaac tttatacata tcctttgccc attgtcctac
     9721 actttggtct aatgtgacca ttaattctgt aaatatattt tgaactctaa caagttcatt
     9781 gtgtgctttt aaatagtctt tatttaaaag tgcaacttta gatatattag tatacttgac
     9841 tgtaccatct acaagcatta atagtaattc ttcttttgat aacccattta gttcatttaa
     9901 atctaactcc atcatttata cctacttctt tcttctctac tttttctatc agcctcatgc
     9961 catgtatctt taatttcttc tattacaggt aatacattat ctattatttg tacatctttt
    10021 ctgatatttg cccttgaaag ttcatatctt acaaaatcat aaactctata catatctttc
    10081 gcccattgac ctgcattttg gtctaatgta accattagtt ctgtaaatat atcttgaact
    10141 cttataagct ctctatgtgc ttttcgtgta tttttatcta taatagctcc tctagctatt
    10201 tttgtatatt ttactgcacc atcaacaagc ataagcaata attgttcttt tgacgccata
    10261 aaaatagcat tttgcttata agaattataa ggattttctc cgtacatata ttacttcctc
    10321 caagtgtttt attttaatga ttttaagatt tttatatttt tatttgtggc ttttatattt
    10381 tcatttttta ctttttcata tatttctttt cttaagatac ttatattatt aggagcagat
    10441 atagctaatt ttatgttatt tccatccact cctacaactt ttacttctat gttatctcct
    10501 ataagtactg cttcatcttt ttttctcgaa attactagca tccaccatca cctctcaata
    10561 atggatgcca tattgaatat tcctcatcct gtaaaattat ttgcattctg caattatttc
    10621 ttacatttat tactatagga gctttcatgt tcacagttga ttcttcaagt gtttgaccaa
    10681 cagtgattat attaagcaac ataatatcat ttggagattt tacttcaagt ttttctattg
    10741 tttcttgatt taggactatt tcatattcct taataatttc aaatggtgat acaacaataa
    10801 atcctataca atcttcttca gtagaattaa attcttttaa aatatcttca ttttctatat
    10861 ctaaaagctc atactctttt aagttttcaa atcctagtat tccttttttt aatgtaatct
    10921 tcatcatatc tgcgttattt tactatatat aatctaatat agatacatta aataactttc
    10981 ctcctgtttg tagtgaagct tgataagtta attctgcagc ctttaattga acaagctttt
    11041 ccccataatc tatttcatca ttttgcgata aaacatcctt taactgtatc aaaccttcat
    11101 catttttttc tttcatggca tctactgtgt ttgcctttgt accatatata gacctaacat
    11161 ttactgtatt atctattaga ttctccatgt cacctaaaca agttgttgtc aactctttta
    11221 tatttgcatc acttggattt gccagtttct tcgatatatt atttaacttt tcaaatggat
    11281 cagtttttgt tggatcaaac ccaagactac tcaacttaac attataatct acactgattc
    11341 cttgagctac atctacactt aaagaatcat ttaatttatt aagatttaag ctagtattaa
    11401 actttaaact tactgagcca tcagcattct ctacacactc aacaggtggt gtgcctgtat
    11461 ttgaacctga aaatatgtac ttaccttcat gtgttgcatt taaagcattt gctatttctt
    11521 tcattttttc attggttgaa ttactaatag tttttatttc gtcatctgaa tatgaaccat
    11581 tcccacttga taccaaaccc ttctttatat ctgctaataa attaccaagt tggtctaatg
    11641 aagtatcagt tgtttctaac caatctgctg tatctttaca attttcaata taagtttcca
    11701 tagtttttat ttcacttttt gaattcaata tctttatcgc cttatatgga ttatctgata
    11761 gtttatttat ttctttcgtt gtgttgattt gtctattgac cttgtctaat ctttgcaaat
    11821 tatcttgcaa tgaatttaga taactactgg acatcatacc tgtactaact ctcattataa
    11881 attcctcctt ttattaaatt atcccattta ttacaacatc caatagttgt actgctgttg
    11941 acataacttt tgcactagct tgataggaac gttgtacttt catgagttct gtcatttctt
    12001 catctaaaga tacacctgaa acactcattc gtgaattatc aacattattt ataattttac
    12061 gttgattcac ttcatcttga ttaacttttt gagaatctaa acctaccgat tctgcaaaag
    12121 ccttgtagtg gtcacttatt gtgcttactt ttccaccagc taccccaata tctatttttt
    12181 catcctttaa cttagcaatc tttgaagctt cttcagtagt aagcttcaat tcatttgaat
    12241 tactttttat tgctggattt acttttataa tatcttcacc agctacattt gaggttataa
    12301 aaaaatctct tacagttatt ccagcagatg aattataaac atcatttatc tgttttgcta
    12361 atccatcaga tacatctttt aagtcgcttt tatatgtatc aattttaccg tgcatttcaa
    12421 acaagccttg taaagttcct gatatacctg ttttccaagt tccatctgca tttttcaact
    12481 cattaagttc caatttacca tctgaaagca tatttttaac atcactattt tcaaggtcta
    12541 acttaaaact caagttatcc aatattctat ctctttcatc aagcaaatca tttggtgtag
    12601 agccagaacc agagataata tcaatagatt tatttaagtc ttcaagactt tttaacatgt
    12661 catttatttg ttttatttca gtatctatat tagaatctat atctcctttt aacttgtcaa
    12721 gcttcttata actttcgctt attaaagtcg ctaaattaga tgcattttct accactagac
    12781 tttttttact atcactattt atatcctttg ataatgactc ccatccttta aaaaaatcat
    12841 ctatacctgc agatattcca tagtctgatg gttcatttag tatagtttct atagtattat
    12901 aataatcata ttttgaaact gtattcccat acttatgagc ttctgcccta aattggtaat
    12961 cataaaaact atttctagct cttgttacat ctgttacatg gactccagta cctaattgtc
    13021 ctcttccaat tgaattgctc aaactaggca tatacattgg actatttgta gcaaattcgg
    13081 tcctttgcct agaataacca tctgtatcta ggttacttat attatgagaa attgtctgta
    13141 ttgatttttg gtgcgcattc attcctgaat tagctgtatt taaaatggaa aataatcctg
    13201 ccatatttct cctcccaata tctattttct aatgtttcca taagaattat aagttgtagt
    13261 ttgtttatca actggtttta tgaaattaat catttttttc gtgaaaataa gttctttctt
    13321 tattagagaa tcatttgatt cttgttgatt tttagctaaa gcaagaatat gctttaagtc
    13381 tatatatatt tcttttaaat attcatcatt actgttttca acaacctcac ttattgaaac
    13441 atcatcccct aaaagttgtc ttctttttat ttctatagat gcaagttctc tactaatatc
    13501 ttctagttta ggattcaacc tatttaaatt ttctaagtct ttttcaatta tataatcaaa
    13561 ttgttctttt agtaattcca tcatattact aattatcttt ttttcttcat atattatgac
    13621 ttttaagtct gtaatcatta ctttatcctc gcatctcctc tatcattttt ttagcaattt
    13681 cttctggttt aattttgtaa gttccatttt ctaatgcact ttttatttct tctatttttt
    13741 tatttctagc cgcttcatcg ctcatatttg atacacttga catttccttt gcaaatttag
    13801 atagttctat tttgtctgtt ttatcaactt tttgaacttg ttgtaccttt tttacatccg
    13861 atttgttctt tagataatta tttactacaa tatttgaact tacactttta atattcataa
    13921 cctcacctcc aaaattacat ccttatattt taattatcgc tcaaagaaga aaaatattta
    13981 tacgattcaa atagatttta aattttttat aaaaataccc cttgattaaa ccaatataga
    14041 ataatctaag gggatatttt ttaaagtatt aatttacgtc tgaatcttct attttagata
    14101 atcttacaga taatgtttca tttgtaataa tactttctcc ataagcaaac aatctgtcat
    14161 ttgcatatat gtctaatttt tcatctacat tcttatttag ttttattata gaaccttctt
    14221 ttaaatctaa tatatttttt attgtttctg ttatgtttcc tatagaaaca gccacattta
    14281 ctttagcatt aaaaacattt tgattttcct ctataatccc agtcttttct tctattaatt
    14341 gaccaaaatt caattcatat atatcttctt ttttgctatt cataaaacta ttctccttat
    14401 gtaatcttat ctatacatat ctatcaataa gccttgtatc atatcaacag tagaaacaat
    14461 atctatattt tctttttctt cactaagtag tttactagtc atatcctcaa gtttgttatc
    14521 aataatcttt acatttttat aatattgatg ctcccagaaa ctactacttt tatcaagtga
    14581 atacatatta tctacaacag attttaggta ttcttttact gcatttttat atctactaat
    14641 gtcaccatag ttttgtgtaa gtacaagcct ctccccagcc ttttttacac catctaaatt
    14701 cctttttata tcttctagtg aagaagattg ttttacaaag tttaagctgt cagaaaactt
    14761 attattttcc tttgtctttt taacttctat atttctactt ggacttatat ttagcttgtc
    14821 tatatttata cccatagtaa atcctaccct ctataatatt tcataacttt aggtatataa
    14881 ttttgtgtct cctttggcat tttatacaag tcagaaattg attctacgcc tctacttctc
    14941 attctaccag gtccaccatt atatgccatc aatcccattt ctatgctacc tccaaacata
    15001 tctatatatt ccttaatatg tctagtacca gcatctatat tttgttctat gttaaaaggg
    15061 tttgttactc ccaaatctct gcaattttct ggcataagtt gcataagacc cttggcacca
    15121 gcagaagata cagtatttgg gttaaaatct gattcaacct taattattgc ctttataaga
    15181 tttgcatcta cattatattt ctttgatgct ttctctacag cattttcaat tcttgacttt
    15241 acacttttat cttttgtatc aacttcaata aacttaccaa ctgtattatt tgctacacta
    15301 tcaagttcat taaacagttt ttcagaatta gatgataaat tatatccatt agaggtttgt
    15361 tgattatttt gtgataatgc tttcaacaat gtcatcataa tcatatcaaa tccagacgaa
    15421 ttattatatc cacagttgca ttgatttgtg gtattactac cagataattg ggtcaatgcc
    15481 agtatattta ttagtgaact tatttcgttc aaaataaaac tcccctttct aaattttata
    15541 aactttaaag ttatccttat ataattcaag atacttaagt tcctataaaa tcttcttgtt
    15601 atagactact ttgttaaatt aatttctttt atattcatta aaactttaga tttagtttca
    15661 ctacattatg attatcggat aaataaaaaa aatatttata ctaaaattat ttactaatta
    15721 tttttttaat tgactaccat acctttttat taatactttc ctttattatg attactatta
    15781 ataaataatc aactttaatt aattataaca tagtatattt taatttaagc tgaactatta
    15841 tttcttatat aaataaaaaa gagatgtaat attaaatgat tatcatcata ttattacatc
    15901 tctttaaatc taaatatcta aattatatat tctagctatc aattaaacat caaagtttta
    15961 tctaaaatac ttaactccag attcaaatat cttctggtct ttttccccta tgatattctt
    16021 atatacttcc tcaccaattc tttcagaatg tcccatttta ccaaacactc taccatcagc
    16081 actagttata ccttcaacag catagaatga accatttgga ttaaactcaa tatcataagt
    16141 agcttcatta ttaaagtcaa catattgagt tgctacttgt ccattagcaa ttaattctct
    16201 cataacatct tcactggcaa caaacttacc ttctccatga gaaattgcta tattatgaat
    16261 atctccaaca ttagtttgag ctaaccaagg agatttatta gatgaaattc tagttctagc
    16321 aatcttagct tgatggcgac caatattatt gtaagtaagt gttggagcac tttcacttgg
    16381 aactcttatt tctccatatg gaactaaccc taatttaatt aatacttgga atccattaca
    16441 gatacctaac attagtccat ctttttgagt taaaaattca tttatagctt cttgcactct
    16501 aggatttcta aatacagtag ctataaattt accagaacca tctggctcat caccagcact
    16561 aaatccacct ggtaacatta ttatttgaga agattttatt tcgtttacta ttgtatctat
    16621 agaatcttct atgtctttat aagttaaatt cttaaatact cttatacttg cattagctcc
    16681 tgctctttca aatgctcttg cagagtcata ttcacagttt gttccaggga atgctggtat
    16741 aaatactctt ggagtagcta tacttaatga tgatttttta gcttctcctt gtgatatata
    16801 acttatagtt tctatctttg attttacttc ttctgtttta gttgggaata taggctctaa
    16861 agtactacaa tgtgctttat ataattcatc taatgcaagt tcttcacctt tgataaatat
    16921 actagcttct ttaactgtac ttccaagcac aacataattg tatccttcta acatacttaa
    16981 atcatcattt gctaattcaa gtactatatt tccataacta gcctcaaata atttatcatc
    17041 tgtaaatgct ttttcagcct cttcactaaa cttaaatcct attttattac caaaagccat
    17101 tttacttatt gcttcaccta cacctgcaaa tcctaatgca tatgttgata aaactttatt
    17161 tccatgtata agttctctta ctttatctaa gtttctcttt aattcctcaa aatctacaac
    17221 atcattttct gccttgttta cacataacat tactactttt gagtctgctt ttttaaattc
    17281 tggagatact actttcttcg catctactgt gtcaacagca aatgaaacta atgttggtgg
    17341 aacttcaata tctttaaatg taccagacat actgtctttt ccacctatag caggtatctc
    17401 aaatttactt tgtgcataaa atgctcctaa aagtgctgag aatggtttac cccatttttc
    17461 tggattgtta ccaagttttt caaaatactc ttggaatgta agtcttgttg tactataatt
    17521 tcctccaata gcaaccaatt tacacacaga ttctactact gcatacaagg caccatggaa
    17581 tgggctccat tttcctacct ttggattgta tccataagtc attattgtag aagtattagt
    17641 ttctcctcca agtactggta tcttagcaac cataccttgt gttggagtcg cttgatactt
    17701 tcctccaaat ggcataagta ctgtattacc accaattgta ttatcaaaca tctctactaa
    17761 tcctttttga gaacaaacat taaggtcaga tagtactttt gtaaacttgt ctttcatact
    17821 tgcacactta acatctttta ctatttcatt agagctaaag aatgtatttt cttcatctac
    17881 tttagtaaca tgaacatcag tagtttgttt tactccgttt gtatctaaaa actctctatt
    17941 tatatctaca attgcttttc cattccagaa cattcttaaa tatcctgtat ctgttactgt
    18001 agctacatgc gtagcttcta agttttcttc tactgctaat tgtataaatt tatctttatt
    18061 ttcttttttt atggctactg ccatacgctc ttgtgactct gaaattgcaa gctcagttcc
    18121 atcaagtcca tcatattttt taggaactaa gtctaagttt atatctaagc tatcagcaat
    18181 ctctcctata gcaacacaaa ctccacctgc tccaaaatca ttacatcttt ttatcatttg
    18241 agcaacttct ttatttctaa agaatctttg tatttttctt tcgttaggag catctccttt
    18301 ttgaacttct gcactacaag ttaagattga ctcttcacta tgttctttag atgaaccagt
    18361 tgctcctcca cagccatctc ttcctgtttt tcctccaaga agaacgatta catctcctgc
    18421 ttcaggtctt tctcttacta cattctcttt tggtgctgct gctataactg cacctatttc
    18481 cattctcttt gcaacaaagt tttcatcata tacttctgca acttgtccag ttgtaagacc
    18541 tatttggtta ccatatgaac tatatccatg agcagcttct gtagttattt ttctttgcat
    18601 aagttttcca ggtaaagtat cttctaaagt cgttcttggg tcagcacttc ctgtaacacg
    18661 cattgcttga taaacataac ttcttcctga taatgggtct cttatagctc ctcctaaaca
    18721 agtagctgct ccaccaaatg gttctatttc agttggatgg ttgtgagttt catttttaaa
    18781 cattactaga tatttttcca tcttaccatc aacttcaaca tctacgttta tactacatgc
    18841 atttatttct tcactttcat ctaggtcatt taatttccca tttttcttta attcttttac
    18901 tgcaacagtg gctatatcca ttaaacatat atctttatgt ctgttcacat atacattgtc
    18961 tcttgagtta agatacattt gatacgcttc ttttactata tcattaaact taccatcttc
    19021 tatctttaca ttttctattt cagtcataaa agtagtatgt ctacaatgat cagaccaata
    19081 agtatctaat acttttattt ctgttatagt tggatttctc ttttctgtat ctttaaagta
    19141 tttttgaacg tgttgtaagt ctcctaaagt cattgctaat cctttttcac ttacaaatgt
    19201 tcttaatcca ttttcatcta aatctataaa tccatctaat acttcaactg ttgttggtat
    19261 ttcagtttcc atttttaacg tttctggttt ttctaaagat gcttctctac tatctactgg
    19321 atttatacag taatctttta ttttagaaaa ctcttcatca gttattttgc ctgataatat
    19381 ataaactttt gctgtattaa tagcaggtct tatcccttga tttacaattt gtacacattg
    19441 agctgcccaa tcacctcttt ggtcgtattg accaggaaga aattctattg caaatactct
    19501 ttcatcattt aattttggta tttcttcata atatactaca tctaaatttg gttcagagaa
    19561 tattgtcttt gctgcatttt cataaacttc ttctgatatt ccttctacat catatctatt
    19621 taatattctt aaattttcta tattatctat gtgtaaactt tctactaagt ctttttttaa
    19681 agctttagcc tcaaggtcaa aaccttctct tttttcaact aatactcttc taaccattga
    19741 gtctttattt tcagtattca acatacctta tcctccattt tagaaaaatt atcttcctat
    19801 gtctgttcta tagtgcattc cttcaaagtt tatcttcttt atgttttcat aaactttttc
    19861 tgctgcatcc ttaactgtag ttgactttgc agttatacca attactcttc ctccattggt
    19921 aaccaaatta ccatcaaaca tcttagtgcc actatggaat actactatat catcatccaa
    19981 gttttcaaga ccagttataa ttttcccttt ttcataactg tctggatacc ctcctgatgt
    20041 aagcataaca cacacagctt catcatcact atagtttatt tctatatctt ttaacttatt
    20101 atctagtata gcttccatta tctcatgtaa atctgtttct aatctaaata aaacagattg
    20161 tgtctcaggg tctccaaatc ttacattata ttcaagtact ttttctccat cttcagttat
    20221 cataagacca acaaataaaa tacctttaaa attaagtcca tcttttttga agccttctaa
    20281 agttttttct aatactgtct cttttacttt atttgctaat tcttctgtat atatttcact
    20341 aggtgaaaaa gttcccattc ccccagtgtt tggaccttgc tcatagttgt ttactttttt
    20401 atggtctttt gcacttgcca taggaactat tgtatcatta tctacaaatg ctagaattga
    20461 agtttctatt ccttttaaaa actcctcaat tactatttta tctccagcag ctccaaactt
    20521 cttatctgac atcatttctt ttaaagttgc tatagcatct tctctatttt caggtattac
    20581 aacacctttt ccagcagcta atccgtccgc ttttattact actggatatc caaaactatc
    20641 tatttctgat atagcttctt ctaagtttgt atattcttta tattttgcag taggtatgtt
    20701 atgttttatc ataaattctt ttgaaaaagc tttacttcct tcaagttgag cacatttttt
    20761 attaggtcca aatactctta atccttcttt ttcaaactca tctactattc ccataactag
    20821 tggtacttct ggtcctacta ttgtcagacc tatttcattt tctttggcaa attttaaaag
    20881 ttcttctata ttagtatcac ctatatttac acattctgct acgtttgcaa ttcctgcatt
    20941 acctggtgca caataaattt tttctacatt tttttctttg cttagtttcc agcatatagc
    21001 atgctctctt ccgccaccgc ctacaaccaa tatcttcata atatcctcct tagtgtttga
    21061 agtgtcttat tcctgtaaat accataacca tatcatactt gtcacaagct tctattgaat
    21121 ctttatcttt tatagagcca cctggttgaa ctactgctga tattccatat tcatgagcta
    21181 atgttacaca atcatcaaat gggaaaaatg cgtctgacgc taaaacagct cctgtaaaat
    21241 ctttatcttt gttattttca agagcatttt ttaatgccca aattcttgat gtttgtccac
    21301 aacctaaagc tagagtttgt ccatttttta ctattgcaat tgcatttgat ttcatatttt
    21361 ttactactct cataccaaat tccatgtcct taagttgtgc atctgttggt ttttcctttg
    21421 ttacattttc acttttttct gctaatatat tgtttctatc ttgtatcaat aattttccat
    21481 ctaaatattt catttcatat ggttgtaaac tattttctat tttagctaat tttaatattc
    21541 ttagattttt cttttgttta agtatctcta aagcttctgg ttcaaaatca tatgctacta
    21601 ctatttctaa gaatatttca tttaatttct ctgctgtagc tttatctata gtagaagtta
    21661 ttcctactat tcctccaaat attgaaacct tatccgcttc ataacatttt gtatatgctt
    21721 caaaagcatc ttttcctaaa cctaccccac atgaattagc atgttttata gctactgaaa
    21781 ctacttcttc actatcttta aattctctca ttacttctaa acatccatgt aaatcattta
    21841 agttgttaaa agaaagttct ttaccgccta attgctcata atttaagatt ggattttttg
    21901 catttgactg tgaatataag aaaccatttt gatgtggatt ttctccatat cttaaagttt
    21961 gttctttttg gaaagttaaa tttaatatgt caggatacgt atctccaact tctccagcaa
    22021 aataactaga tattaacgca tcatatcttc ctgtagttga gaaagcttta tatgataatt
    22081 tttttctgtc tcctaatgtc attgtatctg tttttaattt ttctattata gaatcataat
    22141 cttttatatc tactactact attacatcct tatagttttt ggcagcagaa cgtatcatag
    22201 atggtccacc aatatctata ttttcaatta tttcatcatg actttttcca gcttttaaag
    22261 ttccttcaaa atcatataga tttacagcta ctaaatctat tgaatgtatt ttgtgttcat
    22321 ttacagtttc tacatgagat tctttatctc ttttataaag tataccacca tgtatatatg
    22381 gatttaatgt ttttactctt ccatctaata tttctggaaa atttgtaaca tcttcaactt
    22441 gcattacatt aacaccattt tcttttaatg ttttaaatgt atttcctgta gatataattt
    22501 catagtctaa tttatttaac tctttagcaa attccactac accagtcttg tcagttacac
    22561 ttattaaagc tctcttactc atttataaac actctcctac cttgaagttt taatttattt
    22621 tcacaaaaaa gacttatact ttcttttaaa atcctatgtt caacttctaa cactcttttt
    22681 gcaagtgtct ttgcatcatc atcttgattt accttaacta catcttgcat tatgattggt
    22741 ccagtatcag caccttcatc aacaaaatga actgtagcac cagttacttt aactccgtaa
    22801 tctataacac cttgatgaac tttttctcca tagaatccag ctccacaaaa tgaaggaatt
    22861 aatgatggat gtatgtttat cattttgttt ctaaactcat taaccagctt tgggctgatt
    22921 atttttaaat aaccagccaa aactactaaa tctattttat tttccttaag aatttctatt
    22981 atcttgtctt catcagtttc acaaattgct tttatgttat gatttttagc tctctctagc
    23041 ccataagcac cttgcttact tgagataact actttaacct gaccttttat ttctccagac
    23101 tcagtaccat ctataactgc ttgtaaattt gttcccccac cagatattaa aactcctata
    23161 tttagcataa ttctacacct tcatgactat ctactacttc accaattata taagctttat
    23221 cttctaataa ttcactatat tttttatcta cataatcagc attgtcattt tctctattat
    23281 ttataaagtt tacaacatca tcagcatttt ctttatctac tattaaaact agcccaatac
    23341 ccatgttaaa actcttatga agttctctct catcaacagc attaaaacct tctatcattt
    23401 taaatatagg aggcttctcc caagattttt tattgatatc tagtcctaat cctttaggta
    23461 taactcttgt tatattttct ataagccctc caccagttat gtgagctata gcttttatgt
    23521 catgtttagc taatacatct aaagctaatt taacatatat tttagttggt gttaaaagtg
    23581 cttctccaac tgtcattcct agttcttcaa catattgttc cattttataa ttgtaagttt
    23641 ctaaaaatat ctttcttata aaagaaaatc cattgctgtg tactccactt gaagatatac
    23701 caaccaatac atctcctgat ttaacattgt ttccagaaac aatcttttct ttgtctgcta
    23761 ttcccacaga aaatcctgct aaatcatagt catcttctcc atacattcca ggcatttcag
    23821 cagtttctcc accaattagt gcacaaccag acattttgca accatctgca attccaccaa
    23881 ctattttttc tatatgctca ggcactaatt tcccaagagc tatatagtca aggaagaata
    23941 atggttttgc tccttgacaa attaaatcat tcacgcacat tgcaactaaa tctattccaa
    24001 cagtatcatg cttgtccatc atctgagcta tttttaactt agtcccaaca ccatctgtag
    24061 atgcaagcaa aactggttct ttcataccta caaaatcttt taaactgtat aatccactga
    24121 agttacctaa atctccaact acatttccat cataagttcc ttttatttta ccttttataa
    24181 ggtctactgc tctatttcct tcatctatat ctacacctga ttctttatat gttaacatga
    24241 aagtagttcc tccttatcaa ttttcttaac tgggtattct ccatcaaaac aagctttaca
    24301 gaaagtagac ttatgttctg ttgcatctaa cattccttct atatctagga attttaaact
    24361 atcacatcca atatattctc tcatttcttc tacgttgtta cttgatgcaa ttaatttaga
    24421 gcgatttgga gtatctattc catagtaaca agagtaagct actggaggag aagttattct
    24481 aagatgaatt tcttttgctc ctgcttctct taaagatttt actaattgtt tagatgtagt
    24541 tcctcttaca attgagtcgt ctactaaaat tatagatttt ccttttatta ttgttgaaag
    24601 aggatttaat tttattttta ctgctatttc tctttcttct tgtgtaggtt ttataaatgt
    24661 tcttcctaca tatctatttt taactaagcc ttcacttatt tttaatccac tagcatttgc
    24721 ataaccaata gctcctggcc aacctgaatc tggaactgga actaccatat ctgcctttac
    24781 agtttcattc tgagctaatc tctcaccaca tttaattcta aattcatatg catttacatt
    24841 gtctatagtt gcatcatttc ttgcaaagta tatgtgttca aatatacaac ttttcttaac
    24901 tggtttataa ttttctgaat aaaaatatga ttttagttct ccatctttga ctactattat
    24961 ctctcctggc tctacatctc ttataacttc tccgccaagt atatctattg cacaattttc
    25021 tgatgcaaat atatattcat cacccttttt accaagtaca agaggtctga atccatgtgg
    25081 gtctctaact gcaaccaatt cttcttgact cattattaca agagcatatg caccttttat
    25141 ataatccatt gtcactttta aactttctac tatatcccct ttgtaatatc tagcaagtat
    25201 atataaaatc acttctgtat ctgaattagt ttggaacata tatccatctt cttctagcat
    25261 atcttttaaa taatttgcat taactagatt tccattatga gctatggcta attgtctttt
    25321 tctacaactt ccaactaaag gttgacagtt agaaacatga cttcctccag ctgttgaata
    25381 tctaacatgt ccaatcccta tatttccttt taacttagat aattcatttt ccttaaaaac
    25441 atctccaact aatcccatat cttttttgta cttaatttct ccatcatcta aaagagctaa
    25501 accacagctt tcttgccccc tgtgttgcat agaatataaa gaataatata gttctttagt
    25561 tacatcttta ttagagtaaa tccctaaaac tccacacata catttgcctc ctatttatta
    25621 ttcattcttg aaagtacttc cgtataacct tctacaaggt ctccaaggtc ttttctaaat
    25681 ctgtccttat ctaacttttc atttgtattt acatcccaaa gtctacaagt gtctggtgat
    25741 acttcatctg ccaatattat atttccttca gaatccttac caaattctat tttaaagtca
    25801 actaacttta ggtttaattt taagaaaaat gcttttagta actcgtttat ttttaaagtt
    25861 tcttctctta agaattttaa ctcttctcta gttgctaact tcattgccac tgcataatca
    25921 tcatttagca ttgggtcacc ataagcatca tttttgtagc ttatttcaaa aattggctca
    25981 tcgaaaacta caccttcttc aagaccaact cttttacaga tagaaccagc agcaatattt
    26041 cttactatta cttctaatgg taaaatctct acttttttaa ctaacatttc tctatctgat
    26101 aaacttttta taaagtgagt atttatatta ttttctttta acatttcaaa gattattgtt
    26161 gatatcttat tatttagtat accttttgaa gatatttctg cttttttttc tccattaaat
    26221 gctgttgcat catctttata ataaacaaca tattcatttt cattatctgt agaatatact
    26281 tgttttgctt ttccttcata taataacatt tctgctaccc ccttaaattc ttgtcgtctt
    26341 ctaacacctt ttgtgccatc tcttctctat atgatttcaa atcctctttt aattttgggt
    26401 attttaaagt cattatttgt aatgccaata aagctgcgtt taaacctaag tctattgtaa
    26461 ctgttgcaac tgggattcct tttggcattt gaactattga taaaagtgag tctagtccat
    26521 ccatagttga tgatttaata ggtaatccta ttacaggaat taatgtttgt gaagctatta
    26581 cacctggtaa gtgtgctgct tttcctgctg ctgctattat tacatcagta tcatcttcta
    26641 tttcttttag gaatattgaa agttgttctg gtgttctatg agctgataat gctcttgcta
    26701 caacttctat tccatatttt tcaagcagtt ttatcccttc ttctaacttt ggataatctg
    26761 attttgaacc cataactact gctactttca ttagacttcc cctctccgtt tataagttat
    26821 tttacactac aaatcattcg tattattata cgaattatac accttaaatt ttatattaat
    26881 atacatatat tatcaactct tttttgaaat tttttaaaaa aaatatttct tgtaattttt
    26941 tcattcgcta cgtatcgaac tttatttatg tttttttgtt aaaataaaaa acagattatc
    27001 tcaaaattgg ttttctaatt aaattttaag cccaatactc ttttttaaat cttgtatatt
    27061 attttttatt acttcttttt taattaatac tttgggttta tagtcttgtt ttttagttct
    27121 gaatgttact tttacttaga taaaaccaac acaaagttta ttctttatat aagtaatagc
    27181 taaaatttaa agtaatgata ttatataaat agaaataatt ttatttaaag gagaatctgt
    27241 atgttagaag ttataaaaaa tagacgttca ataagaacat atatagataa aaatatagaa
    27301 gaagataaaa taactgaaat attaaaatca gctatgcaag caccttcatc aaaaaatgct
    27361 caaccttggg aatttattat agtagatgat aaggaactgc tgaaacagct ctcaaagtca
    27421 cagcatagag caaagcatat agaatttgca ccattatgta tagtagtgct tggaaataga
    27481 gataaattct taaaacctgg taaatggata caagatttag gtgcttgcac acaaaattta
    27541 ttattagaag ttactaatca aggtctagct gcttgttggg caggagtatt tcctaaaaat
    27601 aaagtggtaa ataaagttag acaaacttta gacttaccat taaaattagt tccttatgca
    27661 cttatatcta ttggatatag tgaagaaaaa aatgagttta tagaccgatt tgatgaaaat
    27721 aaaatacata gaaatgtata taaaaataga taatgtttag taatataaat gaaaatacat
    27781 agtaaaaatg cctttagaat acatttgtat tctaaaggca ttaactttga ctaaacacct
    27841 aattagcctc taaggtaaaa tttaactatc atttattatt tatataccaa ttgaatatta
    27901 tatccttcaa tatttttatt tatttcaaat atattaagaa tatcctacaa ttaaagttag
    27961 ctatttttca ttaaagtatt ttattgacac actccccata gctaaagcaa ggggattctt
    28021 ggtggctaac acacttattt atacttaaac agtacaacag aggtggttac aaagccaagc
    28081 tacttcggac ggtgtcttcg ccactactac cttaaagtta ttcgcccaca catggctcaa
    28141 acggtagata cttttaaatt tattggttgc ttatgattta ttctaaaatc tactaaacca
    28201 tatttatgct ttttatttaa aatattccaa gcaccaacta tatctctatg agtttcataa
    28261 ccacaagtac aactataagt tctaccagtt ggtttgtgtt ttttagaaca ctttgggcaa
    28321 gtttgagaag tgtagttttc tttaactaac ttaacttcaa taccttgctc ttttgcttta
    28381 tattttatat agtcctttat tcgaccatac tccatttgag atattttctg tctattaact
    28441 ctgtttaatc ttttcttttt cttagtatct ttttcaaccc cttttatatc cccaagaact
    28501 atttcacata ccttctgctc tacacaaaag tctataaact ttctagttgt ttgatgatat
    28561 aaattcatta actgattatc tgttttactt cttatcttat tttttaatct ccataacttc
    28621 tttgattgtc tactaccttt tttacattta cttaatcttt tagatatata agcatacatc
    28681 ttatttctaa attgtttaat acttctgcct tctctattag atagaataag ccctacacct
    28741 tcatttgtta cactagctat cgaatgtatt tcccctaagt ctacacctac ggtattagtg
    28801 cctttggcta caactccatt taattcattg tcaaaagtgt agtataaata atatccattt
    28861 cgccatacta tttcagcagt ttttatattc tcaacattaa aatctaattc taaatacctg
    28921 ccttttgaca ttgataattt taagttgcct ttagcatttc tattaatagc actagcttta
    28981 aaaggtatta tataaaattt cttagtcttg tatggatatt ttgctttagt attacctttt
    29041 tttcttaatt ctgatatagt ctttctattt gcatcaaatt tatctgatat agcttgaata
    29101 gtttgactat gaagattgta cttacctttg attaactttt gtatatcatt ctttttaatc
    29161 cattgtttat gttcaaaata gtattcttta gataagttaa ctatgtagtt ccaacagtta
    29221 gcactttcca tttgcttttc ccttactaat tcaaattgtg ttctagttag gtttaatttc
    29281 actctatgag tttttatcat ttctttgtcc taacttgttg attttctata tattgtttaa
    29341 ctacttctaa actcacagaa ccaacagtac ctacaaaata gctattagtc cacatgctag
    29401 gcatactaga ctttaaatgt ggaaagttct ctcttaatac tctcgaacta aagcctttta
    29461 aaccttttac tactttatga attccatatt gagggtcaca agatattaat aagtgcacat
    29521 gttctttatc agtttccatt tctattattt ctgctccaag ttcatctgct ttatatggta
    29581 gtatttcttt taacatcttt tcaacttcgc caactaaaac aggtttacga tacttagggc
    29641 aaaatacaat atgatatttg caatcataaa ctatattttt gttagattta tacataaaaa
    29701 ctaacacctc ctaaatatag ggtatcagta gcggtattag attacaagca ttattctaac
    29761 tttttttata ttaagacctt acctttcatc cctacaccta aaggagaggg cttttcggca
    29821 attttattgt aaattaataa tgataatcac aactttaaga aaaatatagc ccttaatgat
    29881 tatcataaaa attaattttt tgaaaaaact catattgact cttgaagaag tacatacaaa
    29941 taaattttgc tttatgaata ttcaaataat ttattttata gatatttaat gataaatatc
    30001 ctacaaaatt atattaatat attaggtcga ctaaattttg atataaagag aatataaaat
    30061 actgtaaaaa agtgtaatgg ttatttcctt gaaaagaaag acatactatt aaagcataaa
    30121 ttacaatata ggagatgatt aaatgagaat actttcagaa actgaaaaaa tatctttagc
    30181 agcagttata aaaatggagt ctgacggatt acttatgcaa agagcaataa atatgttaat
    30241 atcagatgaa gacctaaaaa gacaatcaga atctagtata ttagcaacag aaggaagaat
    30301 taaagctatg caacaattta tagttgaaaa tgaaatacta atttctgagg aggtatagat
    30361 aacatgagaa atatgataga aaatctaatt aagaacaata cttatataga cgataaagta
    30421 ataatgttaa gtatgctttc atctgctaaa gcatctgcca atatgtatct taattctgca
    30481 ttgacaagta gtacacctga attaagagct atatattcag cttcattaac tcaaatggta
    30541 gaaggtcaca cagcacttac agaattaagt attaataaag gatgggttaa accatatgat
    30601 aaacctattc aacagttaac atgtagttat aaagaatccc aaaatgttat tgattaatct
    30661 aaataaaata tattcataat tattttattt taactatata ataatataac aaaaaagaga
    30721 tatctcaaaa taaaaatttg agtatctcat tttttgtgta aaaattttta ttccctctaa
    30781 ttcaatagtt atattttgaa agatagttgt attttaaagg tagaatattt attaaaatac
    30841 aataaattat atctatttaa tatgtaaata atttttaata ttagtattta atatttcttt
    30901 ttatatgatt atatactatc tctctataat attctaaaat atcattatct ttaacgtctt
    30961 tatcttcttt tttatcaaaa acagagtcac tgtattcatc aaaattaatg gttccatcta
    31021 tatcagtaat gccttttgtt tctaatatat aacaatattt atcattattg accatagctt
    31081 tataagtctg accaggatat attgattcgc tataggtaaa ctcacgcccc ttacctccta
    31141 gtgtttttgg aagtacacaa tcatacttag ataaatcatg ttctatattt gctaatttta
    31201 aaattatagg gattaaatcc atattactta tatactctgt attaccatta gaagcacttc
    31261 cagaccctct aaccattaat acactattag ttatatttga ttttaataaa aaatcatctt
    31321 tacctaaaaa tgaagctcca tgatctgaaa aaatactaat taaatactct tcttcagagt
    31381 aattttgtaa tatataatca taaattcctt tcaattgacg gtctaattcc ctaagcgcca
    31441 catcatattt tttaatagcc tcttcatcat atttttgcca tgcacttaca aatgaactcc
    31501 tttttgataa agatgtctct atatctaaag atgtttgatt aacaatatca taattaacac
    31561 tataattttc aatatcatcc aatattttat gaacatcact caaagacata aacacaaatt
    31621 gattggtttc tttaaatgat tcaatatgct taatcgcatc attaattatt actgaatcag
    31681 aaaatcctaa actattctta aaaacagttc tatcaaatcc tctaaaacca ctgctatatg
    31741 gtgcaagtgc atatgtacca cttatatttg cagtgaaata ttcatttttt ttcataatct
    31801 cactgaacaa atctatatca tcagggtatt tatatcctgc actagaatgt aaaaaatgat
    31861 gattagttgt atataaccct gtaaccacac ttgtcaaaga tggatatgtc cactctcctg
    31921 ctgaataaca attttcaaat ataattcctt tgtcaaaaaa agcttttgtg taaggcattg
    31981 atttatattt ggtttccaat aaatattttt gagataaagc atctataaat atagtgaaaa
    32041 ctaatttttt atttttttct ttaaacccta gctttattgg cttaccaatt ataacatctt
    32101 tatctgaatt taaactaatg ccattattta ctctataata cttaaaaaaa ccaggtggat
    32161 ctacttgttg tgatttaaaa ttagtatttt taatcttaaa gtcaataact tggttatatt
    32221 cagttgtagc aattggaata atatacttcc cattatctaa actaaattct tttgcagtca
    32281 tagatttaaa actttctatt ggacatattg aagatatctc agaattgtta ataacagtat
    32341 ataatttttt ttgaaaatct gataacatat tattataagt gtcagaataa aaacatatat
    32401 aatcattata ctcactattt aagtccttat ttaaatattc tcccactaaa atattccatt
    32461 tatccccata aaagcagaca ggaaatttag caatacttag ttcaataaaa ccaaaaaatc
    32521 tttttaaatc ttcaagtaca tttgtatctt ttatattttc aatgcagtac tcaataagag
    32581 tttttacttg ttcattataa taaaatagct tgtcatttat ataatcatat ccctgaatct
    32641 tagcaaaatg ttttaagaat gtcacactta acacagctga tttaatggca tcaggatatt
    32701 cacccatagc aatcaatata tttgctaaat aaaaattatt atcataatta tttggtctaa
    32761 atttcaatgc ttttctaata tgttcttctg caagtttata ttcctctcca aaaaaatatg
    32821 taactgcacg tgcagtatga atctcactat caaatggtag tagcttctca tactcattaa
    32881 tataatctag tgatgttttt atatcaaaac gctcttggtt cattatttca tctaaaattc
    32941 ccatacaaat cccttcctaa cctacttaga gtattgtaaa attttcttca ttgtaaattt
    33001 attattttgc actatacatt tttccgaaaa tctcaaatct ttttcattat ctatttcaaa
    33061 acaatcttta aaatctattt tctgaatgta catatttaaa gcatctttat ttttcattac
    33121 aacatcatcc caaaatagac tttcaaagtc tccattttct attgtttcct ctaatttttt
    33181 tgctataata tttccatctg ttaatgacca ataggataca ccacataata tatacccctt
    33241 attatcacca acatacatat cataaacctt attattttca tcaaatttaa atataaactc
    33301 attgttaaaa ttttctttat aaagagtaaa ataagtggat tcctttatat ctggatctat
    33361 aaagttatta catatatata catctccctc tataacatag ctatcaggta aatgttctct
    33421 taataaatac atagtataaa tattattgta tatatcatat ttattgttat atattaattc
    33481 cacattatat ttttccgcta aataataaaa tttttctttt aaataaccta ctgcaacttt
    33541 tatatctttt atcccctttt ctaatagaca ttctatttgg gtttctatca ttggcttccc
    33601 attaacttta actaatggtt ttgggcactc tgaagttaat ggttttaatc gtgtccccag
    33661 cccagctgct aaaattattg ctttcatatt taatcctacc ttccaattga atattgtaat
    33721 gttttagagg ttttaagtta gtatcactta taaaatctcc aaatattccg atagaaattt
    33781 tttattagta
//
LOCUS       DAEMHZ010000033        33497 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000033 DAEMHZ010000000
VERSION     DAEMHZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.033"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(81..692)
                     /locus_tag="KPK72_001427"
     CDS             complement(81..692)
                     /locus_tag="KPK72_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2488657.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(784..1773)
                     /locus_tag="KPK72_001428"
     CDS             complement(784..1773)
                     /locus_tag="KPK72_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG2488658.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(1840..2703)
                     /locus_tag="KPK72_001429"
     CDS             complement(1840..2703)
                     /locus_tag="KPK72_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG2488659.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(2809..3405)
                     /locus_tag="KPK72_001430"
     CDS             complement(2809..3405)
                     /locus_tag="KPK72_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG2488660.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(3445..4248)
                     /locus_tag="KPK72_001431"
     CDS             complement(3445..4248)
                     /locus_tag="KPK72_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG2488661.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKIGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(4284..4724)
                     /locus_tag="KPK72_001432"
     CDS             complement(4284..4724)
                     /locus_tag="KPK72_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG2488662.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(4737..6839)
                     /locus_tag="KPK72_001433"
     CDS             complement(4737..6839)
                     /locus_tag="KPK72_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG2488663.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPNDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEIKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(7052..8755)
                     /locus_tag="KPK72_001434"
     CDS             complement(7052..8755)
                     /locus_tag="KPK72_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG2488664.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(8781..9740)
                     /locus_tag="KPK72_001435"
     CDS             complement(8781..9740)
                     /locus_tag="KPK72_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2488665.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(9876..10394)
                     /locus_tag="KPK72_001436"
     CDS             complement(9876..10394)
                     /locus_tag="KPK72_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG2488666.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(10437..10922)
                     /locus_tag="KPK72_001437"
     CDS             complement(10437..10922)
                     /locus_tag="KPK72_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG2488667.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(10919..11536)
                     /locus_tag="KPK72_001438"
     CDS             complement(10919..11536)
                     /locus_tag="KPK72_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG2488668.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(11601..11963)
                     /locus_tag="KPK72_001439"
     CDS             complement(11601..11963)
                     /locus_tag="KPK72_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2488669.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            complement(12707..13204)
                     /locus_tag="KPK72_001440"
     CDS             complement(12707..13204)
                     /locus_tag="KPK72_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG2488670.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(13379..14290)
                     /locus_tag="KPK72_001441"
     CDS             complement(13379..14290)
                     /locus_tag="KPK72_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2488671.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            15242..16093
                     /locus_tag="KPK72_001442"
     CDS             15242..16093
                     /locus_tag="KPK72_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2488672.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            16097..16567
                     /locus_tag="KPK72_001443"
     CDS             16097..16567
                     /locus_tag="KPK72_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488673.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            16580..17890
                     /locus_tag="KPK72_001444"
     CDS             16580..17890
                     /locus_tag="KPK72_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2488674.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            18102..18848
                     /locus_tag="KPK72_001445"
     CDS             18102..18848
                     /locus_tag="KPK72_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2488675.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENCFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            19210..20751
                     /locus_tag="KPK72_001446"
     CDS             19210..20751
                     /locus_tag="KPK72_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2488676.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMLPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            20786..21892
                     /locus_tag="KPK72_001447"
     CDS             20786..21892
                     /locus_tag="KPK72_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2488677.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(22271..23557)
                     /locus_tag="KPK72_001448"
     CDS             complement(22271..23557)
                     /locus_tag="KPK72_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2488678.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(23680..26778)
                     /locus_tag="KPK72_001449"
     CDS             complement(23680..26778)
                     /locus_tag="KPK72_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2488679.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKIISKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            27504..27662
                     /locus_tag="KPK72_001450"
     CDS             27504..27662
                     /locus_tag="KPK72_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488680.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(27949..28653)
                     /locus_tag="KPK72_001451"
     CDS             complement(27949..28653)
                     /locus_tag="KPK72_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2488681.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(28644..30251)
                     /locus_tag="KPK72_001452"
     CDS             complement(28644..30251)
                     /locus_tag="KPK72_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG2488682.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(30505..31683)
                     /locus_tag="KPK72_001453"
     CDS             complement(30505..31683)
                     /locus_tag="KPK72_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488683.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(31909..32403)
                     /locus_tag="KPK72_001454"
     CDS             complement(31909..32403)
                     /locus_tag="KPK72_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2488684.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(32404..33165)
                     /locus_tag="KPK72_001455"
     CDS             complement(32404..33165)
                     /locus_tag="KPK72_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2488685.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT        10996 a         5760 c         3747 g        12994 t
ORIGIN      
        1 tatactttga aaataaaata aactatcttc caaacatttt attatatgct taaaagatag
       61 ttttgtgtct ttaaaataaa ttaaattgta tatattcctt cacataactt ttgtatatta
      121 ctaggtggtt gccatttacc atctttttta gtatatgtaa tcatagttgt aacttcctta
      181 gttggtttct tattgtaaag gtctataatc atttttgtca tatcatcttg aacttctttt
      241 tcttttttat cgtaattctt ttcatcagtt acataagatt ttactaaagt gttcgcttca
      301 ctcattactt tactagtatc taaagcttta cttacaactt tcaaagttat cttatcctta
      361 ctagaagatt ccactgttac tgtcttttca acaccttgaa ttaatttaac aattgtttcg
      421 tatatctttt cagcattttc tttaccataa agaggtgtca attgagcaat cattgaaacc
      481 ttgtccttgc tgttataagt ttgaatctgt ttatattcag cttctgttaa tgcatcagaa
      541 attccttttt catcatactt atacatttct aatgcagcta aattagcttc aaaattcgac
      601 tttacttttt cttctgcatt agaacaccct atagttagaa ctaaaactag agccataatc
      661 gtagataata ttaacttttt taccattttc atacaaacat cctccctcaa ttaaaataat
      721 aaaaaattta tttatcaaca ttataacata aatttgtcta actatagaat tattttgaat
      781 attttaccac ttccagtcta ttccaatacc gttttctttt gctttatcgt atattctttt
      841 tgctgttacg acatcttgta cacctatccc aacagtttta aatacaatta tctcatcatc
      901 atcttctctt ccaggaatag ttccgtttat aacattccct aaatcaccac taaaatcatc
      961 tttacttatc ttaccttctt ttaatggaat taatatatct cctgactcag ataaaactgc
     1021 atctgttgat tcaaaaaata tcttagacgc tctagttaaa cagtttgggc acaactcttg
     1081 catatctggc atatatgaac ctactgcact tattaaagct ccttttttca ctaagtttcc
     1141 attaagtact ggttgttttg atggagtagc taaaactatc acatctgcgt tatctatggc
     1201 ttcatcagat gattcaactg caactatttt agtattatat ttacttaatt ttaagttcat
     1261 ttcttctgca aatttttctc tactttcctt gtttctacta aaaactctta cctcatccaa
     1321 atttctagct tctatcatgg cttcaagttg tggctcacct tgtcctccta gacctatcaa
     1381 agctccaatc ttagaatctt ttttagccaa tacatctata gctgctccag aggctgctcc
     1441 agtacgaatc tgagttacat aagtaccatc caatactgca ctaacttctc cagtttctcc
     1501 atctaataat agtactgttg ctggaattac tggtttgcct ttttgagcat ttttaggaaa
     1561 tacagataca atttttattc ccccacagcc taattcttct acatatcctg gcataaataa
     1621 catactagca tcttgacttg gaacagatat attggttctc aaaggattta cactctttcc
     1681 ttcacaataa attctaaaag cttccttatc agcttcaatt gcatctctca ttgtaaatac
     1741 tttttttatg tcgtcttttt ttaaaagtag catttttata cccccaattt taataagtct
     1801 ctatgctaaa tattatagca taacatttaa tttaatcttc tatttcaaat caaataaatc
     1861 tacatactct ggttttagca tagaacttat caacgcttta tttattgaaa attctggtat
     1921 tcctgctgca tatggagtta tatcatataa atcaaaatat acaactagat tctcatcttg
     1981 caagtaaaaa ttttgattct cttttatacc tttaaaatta tatattccga tattttcttc
     2041 atcctttttt tcaagttcag ctatttgtct tttaatttct tcatctataa cttctttata
     2101 tttagtatta tccttaaaca agtccattaa ttctaaaaat tttccagttc tcatatccac
     2161 attatatgct atatcttgat aaaatccatg tgctccacca ctataagtat aatatctcac
     2221 ttttatacta agagcagaat cagtattctt tttaagttca aagtctacat ttgcaacaaa
     2281 cttatttttc tcgtcaggat tatccttaag atactgcttt gcttctttat atgatttatt
     2341 ataaaattca aatatgtcat tttttatctt atcatttgta gacctttcaa caattttatt
     2401 tgaatttgtt attacaggca tgtttatagt actttctaaa tactcatcat ttttcactat
     2461 tgttttactt cctattgtac ttactgataa agtttgttta ctatttgtat taccctttgg
     2521 taatgactgg ttactcataa cagtcactgc ttccttatta ctatttaaat taattgaagc
     2581 atctttcctt aaattattat atttatagtc atgtttaatt gccaaaacag ccattatagt
     2641 caatgttaaa acaaatacat atcttaaaac tttagctttt gtatcgactt taaaaaattt
     2701 catattatca ttttcctcct aaatataaat ctctcttatt atatttggag tttctagggt
     2761 tttttatcca tataatttaa taaaagatta atttttattt ataaaaaatt aatctttttg
     2821 ttttaaaata ttaatatgtc ttatcaaaca gtttgaaatt tcaccaagag gtaattgtaa
     2881 cataacagaa cctctatcaa aagctaccat tggcattcca tatactatac aagaattttc
     2941 atcctgtcca attgtaaatg aaccattttc tttcattttt aaaagtccac ttgctccatc
     3001 ataacccata cctgtaagta ttattcctat tgaattttta cctgctactt ctgctactga
     3061 atcaaacaaa acgtctactg atggacagtg accacttatt ctttctcctt tttcacaagt
     3121 tacataatat ccttgtttat ccgtacacaa tttcatctgg taacccccag gtgcaattaa
     3181 cacatttcct tgttcgactc tatcattatt cttagcttct cttacattca taatacactg
     3241 cttgtcaagc cgttgtgcat acatataagt aaatatagct ggcatatgtt gaactacaac
     3301 aatccctggt gttgattttg gtaggtcttt tataatttca agtattgctt ctgtaccacc
     3361 tacagatgcg cctatagcaa taaccatatt attaaaacgt tccacattac cgcctccaaa
     3421 aaatcatata tcaaaaatac ctttttatcg tttatatata gcaggtttta catatttgta
     3481 tttagtacta cttctgttga ttacttctgc atgtcctaca aacaaaaaac tatctttttt
     3541 ggtagcatta taaaatttat ttataagttc ttctcttgtt tttaaatcaa aataaatcat
     3601 aacatttcta caaaaaatca aatcaaaatg ttgatatgag aaatcattca ttaaattaaa
     3661 cggcttaaaa attacacttt ttctaatctt atcacaaatt ttaaatgtac catccttgtt
     3721 gtctataaaa tacttcttct tccataactc aggaacattg ttaacatttg attctatgta
     3781 tatacctctt ttagcttttt caagtgagtt agttgaaata tctgtggcta tgattttaat
     3841 gttccacaac tctttattat atccaaaata atcatccaaa gtcatagcta ttgaatacgc
     3901 ttcttctcca ctagaacatc ctgcactcca tatatttatg tttcttattt tattattttc
     3961 ttcccaaaaa ggaagtatac tattctgtat aaactcaaaa tgttgaggtt ctctcataaa
     4021 aaatgtgtgg ttagtactca atctatttat taggtttata gcttcatttc ctatcttatc
     4081 tttaaataat agattcatat attgactaaa agaactaagt tttttctcta tcattgtatt
     4141 gtataagcga ccttcaatta acgctctttt tttacttaaa tcaataccat attctttttt
     4201 tacatggttt acaagaacta taaattcttc atctgtgagt tttatcatgc aaaaacttcc
     4261 tcctgctaat ttatcagaac taactatatg ctattaatca aaaactttcg aatatctaaa
     4321 attaatatag aattatcttc aactttcgca atctctttta tatctttatt aatattagtg
     4381 ttgttagaat tagtcgtctc tagtaaattt agattttcta tatgaataac atcaacaact
     4441 ttatctacaa taattccaac ttgatactca tcaacctttg taattattat acaagttctc
     4501 tcattataat cagctttagg aagattgtac tttatatgca tattaataac tggtattatt
     4561 cttcctctca aattaataac tccacttata tatgttggaa gacatggtac atacgttatt
     4621 gattgaaatc ttatggtttc aatcacattt tctaattcta ttccataaat aacatcattg
     4681 acataaaatg ttaatacttg tttaataatt tcatctttag ccatacttta aatcccctaa
     4741 tatatttcta taatgctatc tgcatctata attaaactta tacttccatc cccaagtatt
     4801 gtacatcccg ataaaccctt attttttata ttaaatcggt taaaataaac tggaaatggt
     4861 ttcacaacaa cttgctgatt acctattatt tcatcaacaa aaatgcatac tgctttatgc
     4921 tcattttcaa taattatcat attaccattc atcagattgg ttacttttgt ttgaatatta
     4981 tatattttat gtaaccttat aatgggaata catgctccat atattgtaat taactctgtc
     5041 ttatttcctt ctgtaattat ttgatttaag tctgtaagtt taaatgttct tcttatggat
     5101 gatattggta aagtaaatag tgaatctcca actttaaact tcataccctc tactatagaa
     5161 agagttaatg gtatttttat agtaaaggtt gtcccttcat cctgtttact ttttacttca
     5221 attgaacctc caactttttc aatactttta cgaacaatgt ccatgcctac tcctcttcca
     5281 gaaaattcag ttatagattc ttttgtagaa aagcctggag tcataatcaa agaattgacc
     5341 tcgtcatcag tatactcatt actttgatgt ttaagaagac cttgttcctt agcttttgcc
     5401 aaaatcttat ctctcataat gcctctacca tcatctgaaa tacttatcac agcttctccg
     5461 cctagatttt cagcagataa aactatcttt cccacttctt ctttaccttt cgctatacgc
     5521 tcctcaatag gctctattgc atggtccata gcatttctta taaggtgcat aaagggttct
     5581 gctaaattat caattacact cttatctacc tctgtctctg ctcctatagt tattaactct
     5641 acatttttat ctaatttttt attcatatct cgtacaattc tatgcatttt ttgaaataca
     5701 ccagcaattt gaatcatacg catagacatt actatatctt gtaattcatc tgtaagcttg
     5761 tgtaattgtc ttgttgcctt attgaaatta tctatatcaa cacctgtaga atttacgcta
     5821 tttacaacca ttgattccat aattacaatt tctccaacta tattttgaag taagtccaat
     5881 ttttctaaat ttacactcat aatactctgc ttacttattg gtaaatgttc attttcttca
     5941 cttagactat ctttttccat atcagttttt tcatttaaaa catcttcttg tatctcatct
     6001 tctgtatagt tattttgata acttttactt actttattat tatcttttaa tttattttct
     6061 actgcatcat tgtttgatgt attttttatt tctatttctt ttttactaat atttatgtat
     6121 tcttttacat agagattatc ttctatagtt ttataaacta ttgccttatc ctctttagct
     6181 acaaattgta tataaaaccc ataactttta atatactctg tagactctgc atttgtttct
     6241 acatcatttg gataaaaatc tataaattca cagtaatctc taagttcaaa tacaagcatg
     6301 aaagctctca cattttccat catgcaacct tgttcaaaga ttatttttac tacatcagag
     6361 tcttctttgt attcaaaatt tgaatcgatt acttcttttt cgtctgtact ttctaaattt
     6421 gcattatcct ttgatttcat agaacttgat aattccaaag tactcacata ctcttcaatt
     6481 ccttcaataa aattagagta atcagaagat atacaacctc ttgattgtaa ctcatctata
     6541 tctcttctta taaaatctaa tgattgaaag actaaatcaa ataataggtc attgtctaaa
     6601 acttctattt taccttcacg tatagcataa aacaagtctt ctacactatg tgctaaagca
     6661 gatatattgt ctaaccccat catagatgct gaacctttta tcgtatgcat tattctaaaa
     6721 gtttcattaa tgttgttatg agtaaatact ttctcttttt ctatttgaat cataatttca
     6781 tctaattgtt cgagtaataa acttgtctca aatataaaca tatctaacat ttgtctcata
     6841 ctcggttcaa tataattcac atttatcacc tacgacctac aaataatttt ctattatata
     6901 tgcatctcat aatttaaatc cttgatgatt ctcttatctc actatatgta cctcataatt
     6961 taaatacaca atcattctct tatttcaata tatatacatc tcataattta aatacatgat
     7021 aattctctta cttcaatatt cacctcataa tttaaaccca tgatgattct cttacttcac
     7081 tttcctttag atgaaatttt tctactaatt cttttaaaat ttgagcttga gcagacatct
     7141 cttctgaggc tgctgcactt tcctctgctg ttgctgagtt catttggact actatggaaa
     7201 tttgctctat tccagataaa atctgtgtaa ttgctacttc ttgactatgt acagccgaag
     7261 aaataccatt aatataatta gtagtttttt ctacaccatt tactaccaag tccaatgcat
     7321 ttgcagtctc tttagcaatt ctgacaccat tttctactgt tttaatagct tcttctatga
     7381 gaatagctgt attttgagct gcttttgcac ttttaccagc caaagaccta acttcatctg
     7441 ctacaactgc aaatcctcta ccatattgac ctgctcttgc tgcttcaaca gctgcattta
     7501 atgcaagaat attagtttgg aatgctatct catctattgt tttgattatt tttgatattt
     7561 cactcgattt ttcacttatt tcacccattg atgtcatcat atcatccata catctacttc
     7621 cgaaaccaac ttgtgatgca gattggtcag atacctcaca tgcaagacta gcactatctg
     7681 cacacttctt aattccatct gatatctcat taatcgtgga agctaattct tctactgaac
     7741 tagcttgttc agtagcaccc tgtgaaagag cttgtgcacc tgctgcaact tgttctgaag
     7801 cacttgcaac ttgttctgct gataaattta tttcagaaaa atcaccatta aaagagttta
     7861 ttatactttc taaagatatt ttaattgatt taaaatcacc tataaattct tggtctatct
     7921 ctactcttaa atctcctttt gcaatactag ttaatgtact gtctataagg tctatgtaat
     7981 ttaaaagcat actttgcatc tttttgacag aactcactaa tagccctatt tcagatttac
     8041 tttcttcaac gtctatactt gttcttaagt ccccagatgc aattcttgaa aagtgttttt
     8101 ctactttatt aataggaatc actattttta attttattac tgtatattct attattgtca
     8161 atattattaa aagggaaagt ataaatattg ttcttaatat tagactttgt aattcttttt
     8221 ttacttctac aattttggaa tttgaacggg taactatatc ctcttgaaat tcatttatta
     8281 aagaatccat tttctcttgg gagtttatat agtcactacc aaaaattata ctttgagctt
     8341 tactcttatc attactttcg actgccttta ttgcactttg ttctgttggt actaaagaat
     8401 ctgatatttc ttttgcttct aagattttat ttgactcgct ctttgtaaga ccaatttttt
     8461 ctaatttgtt taaagctgat tctcttgttt ttccgtataa ttctttttca taaatatctt
     8521 tattcttttt ttctcctgta aatacatatc ttcttaatac atcacttatc tctctagttg
     8581 tatcttttat atactgactt tgagtcctca aattattcat atcattaata cttttttcta
     8641 ctttttcaaa tgctttataa gatgaaatag aagaaaaaat ccctgctaga acagccaata
     8701 ctataataat caacaataca attgctaatt tagaaagctt tacttctctt ttcatatctg
     8761 tactccttca taaagtaagt ttatatcttt tttaatcctt tcattgtact cactacgcta
     8821 caatcttttc ctaagttttt accctcatac attgctttat ctgctaagga tatgttttct
     8881 tctaatgata tatttgagtt tacttctact actcctaatg taatagtgca ctttatttta
     8941 tgttctttat agttgaaatc aaactccttt atctctttac ggattctttc tgaaagtatc
     9001 ttagcacctt ctattggagt atttgtgagc attatcaaaa actcttctcc accccatctt
     9061 gatacagtat caatttgtct acaattcttt tttattatac ttgatattac tgttaacacg
     9121 aaatcccctg cttcatgccc ataagtatca ttgataacct taaagtcatc aatatcacca
     9181 aggataaggg atattgatgt ttcactatat ttattaattc ttctttgctc cataagctga
     9241 ttaattatat gtcgcctatt gtaaagacct gtaagataat ctcttacaac gagtctattc
     9301 aatttttcaa gagaagattc cagttcatta tttatctttt ttaatttatc ttgagatttc
     9361 tttacttcaa gatgagctat aacacgagct tttaattctg tcatactaaa aggtttcacc
     9421 atatagtcaa ttgctcctac accaaatcct tttactatgt ctgtatctgt attttttgaa
     9481 gttataaaaa ttataggtat atctgctgta cgagcattag attttacttc cttacaaaac
     9541 tcaaaaccag agttatctgg taaaattata tcaagcaaaa taatgtctgg tataaattct
     9601 tcaataaatt cttttgcctc tattgcatta aatgcttttt taaccttaaa attatctttt
     9661 gtaagtgttt tatctattaa gtcacacatc agaacactat catcaacgac taaaattgtt
     9721 ccctgttgct tatttttcat atacaaacct ctcttaaaca taatatattc aaattttaat
     9781 gatttaaaat ttttatactt aataattata ccactatatt cttaaatgtt ctcatcacaa
     9841 tctttttata ataacactct aactacatcc ctatgttaca tgatagtgca atcaaaccca
     9901 ttgattaatt catctattaa aacttcatct ttatctgtca aaagtttctc taaatctagt
     9961 aaagtaatta ttttaccttc taattctcca actccactta caaaataatc cgaatattct
    10021 atagtaactc ttggtggagg tgatatttta cttttttcta agcttactat atcatcaact
    10081 ctatcaacca caaatccaac taccaagtcc tttatttcag ttactactat atacatgcaa
    10141 tttgtagtca ttaattcatc ttttttaagt ctcaaacgca tatctattat cggaatgatt
    10201 tttcctctta gatttattat gcctttagaa tatgtaggta gttctggaat aaatataatt
    10261 tcttgatttc cagtaatttc tattaccttt tcaagttcaa ccccaaaatc ttgctcatct
    10321 actttaaact ttagatactt aattccttca tcaattagtc cataatctaa atatgggtca
    10381 tctggaaatc tcatttaata cctccctcct aaaatacact ttctatgaaa tatatattat
    10441 aatttcttct tacctttatt tattgaatct acaagtaatt cacccgtctc agtgtaaaaa
    10501 cgtattgttc taccataatt catacctgtg tcctcagcta atattggaat atttaatcct
    10561 aaaagagttt tttttactgc ctcaacattc ctatgtccta tacttgcaat tactgaattg
    10621 ctacttccta aaaacatctg agcacctcca gcaatttttg cttttatata tattctattt
    10681 gcacctatat tctccataga tttaatgagc tcatttattc ctgtgtcagc aaatttacac
    10741 ttattttcaa tgttaatagc atctttacta tatggcaaca taacgtgtaa cattccacca
    10801 attccagcta ctttatctat caaacatact cctacacaag aaccaagtgc ataagtaact
    10861 aaaacatttg gtctataggc tatcttcata tctgcaatgt tcactactat ctctctattc
    10921 atacttctaa tcccaacctc acgagtattt ctctaagtga gctcatttct ggaatcatca
    10981 aaatgtgact agtaaaataa ttatcactca ttttaaattt attttctata aataagactt
    11041 tgtcaccaac atctgaaaat cttatcatag gtacatttag aacagctcca accatatcaa
    11101 tacatatgtc tggaattgaa acacttatag tcatgtttaa catagatgca atagcattta
    11161 catatgaact tgccataata tttcctattt ccttaattgc tgaaatttca attgcctcaa
    11221 tctcatctaa aaaagcttct tctttattaa aaagagaact tagcataagt tgagttattg
    11281 attcatccaa caaaaacata aacattccat tgatatcacc tttcatatcg agtaaaatac
    11341 atactacttt gttttctgga ccaccaagca aatttgtcgc ttcattatat tcaagtatct
    11401 tgaccatagg tattaatact tctacattgc tatttatcat ggatgcaagt gcagtaattg
    11461 cattcccaga gcctatatta ccaatttctc taagagcatc tatatgaaca tcatcaagtt
    11521 ctaaataatt tataatataa acccctccct ttattattgc atttatttgc ttttcattgt
    11581 atcagtgtat atttacactt ttattttaaa acattattta caactttaac taatgtttct
    11641 gttttgaagg gcttcactat aaaatctaac gctccaagtt ttatggcttc tacaatcata
    11701 ttctcttgtc ctattgcaga gcacattaca accttagcac ttgaatccaa cttttttatt
    11761 tctcttagca cctctaaacc atcttttttt ggcattgtaa tatcaagcaa catcaaatca
    11821 ggcttttcat atttaaatat attacacgct tcttctccat catttgcttc tattaaatta
    11881 ttatagccac ttttactcag agcttcttta attatcattc tcataaaaat agcatcatct
    11941 actatcaaaa ttttattttc cataaaacta actcctccat atgtacaaca ataaacgctt
    12001 ttatcttcag ttattgtcta tactatctga ctatttttcg ctacacaata aatacatttc
    12061 attgataaat aaaagtaatt atttatgcca tattatactt aattctctta acataatttg
    12121 tcaacaaaaa aacttatata tctattgtaa tatatgtttg gcgttttgac gagttattaa
    12181 aaatataatt atataaaaca taaaaagtta tcaaaagtaa ttaaattata ataaaacaac
    12241 tttatacctt ttttatgttt gtataaaaaa atttttcata ttcattaaaa tcatttctgt
    12301 agattcatat aggatttgac tttctcaata tctaatagat agttgtttat ttacttaatt
    12361 atatttctct atatgtatta tcgattaaat tagatttagc tttatacatt attttttata
    12421 tttttcaaat tgtatactca taaatccaga agtattctaa tgtgtttata atactttttt
    12481 tcatatttta aattaaataa caaatcatta tatttacaag tatcatttat caataaacaa
    12541 tattaaatga agaatatgtc tgaagattca aagtgatact gataatatta attactaaaa
    12601 aagcagtaaa tcttttacag atttactgct tatagttata aataataaaa ctatctataa
    12661 taaatgtact atactcaata cttcattcaa tatagtaata acaaatctac tttattccta
    12721 tgtaaatatg aacttcttgt ttctttgtat caaaagtatt acattgatac tcttcaaaat
    12781 cacttatata agtcctatca aaatccattt gccaaaactt aaaccaaaaa tctcctactt
    12841 ctttttgacc tcctactata acaaactttg catacttacc tgctggtata actttactaa
    12901 taatactttc tccaacagta ttattagtgt ttaattcttc taaattcttc tctttatcag
    12961 aattactatt tacttcacaa catgcaacaa agctatacgg acttgtaaaa tcaccctgat
    13021 aatctgtata tagacctatt gtcttgtcat tctttttacc ctttatagca ttatatatcc
    13081 ctttgcccat aaattttttc cacaattcac ctatatcatt taccgcttgt ccatctttat
    13141 ttgtagtctc cttcgatatt cctataacta ttttctcatt tatctctact atttcataat
    13201 tcatacttta tacctcactt tgtttttatt aatatatttt attttgaact aattcaatta
    13261 taatttataa tatacgacac ctttatgtca tatttttaat ataattaaac ccagtgagat
    13321 attatatact aaatgcaaga tattatatat ttaataaaat ctttaatcta gcattatatt
    13381 ataatgaata aattttagac aactccctta gtctagttat tacattatcc ctaacatctt
    13441 taggttcaag tacttcaaca ttgtcaccaa atgataaaat atatccataa atccattcac
    13501 caataggaaa ttctgtaatt acatcatatg tatcatcttc atttttagtt attaaatcct
    13561 tattaaaatc atcaaatact ctaaaaagca tcttttcttt aaacctcaat ttcaacgtta
    13621 tagtattttc cttaattact ttagaatcat tcaaacatac ttcttctatt atctttcttg
    13681 aaaacacttc atcctttata gataaattcc ttatcctact aattctaaat atcctaaaat
    13741 catcttttag tttacaaaat ccatacaagt accaagtctt actcttatac ataagcttca
    13801 gtggttcgac agttctgttt gtctggcttg acaaactatt tacataatta aaatttatag
    13861 tattacgcct taaaatagct tcttttattt ttgtaaattt atcatcttca ttaaaattac
    13921 ttccccattc agtaaagtct atttcaatcc agtttgaaaa attctgctct ccaaatattg
    13981 agccaatttt atttactact gaatttattt ctggatactt agtcgcctgt aatgttttca
    14041 gtgcaactat taaactctct ttatcttttt ttgacaatat agccttgtct atagaatatt
    14101 cttctattaa agatatccct cctcctttgc ctttcgtcat atatactggt actcccgaca
    14161 tagacagagt ttctatatct ctatatatgg ttcttgtaga aacttcaaac ctatctgcca
    14221 actctttagc tgttactaat tttttattta atagaattac tattatttct gttaatctat
    14281 ttattttcat tcattaccac ctactaaaat tattaaattt atttaatcta aattaatcat
    14341 actaattttt aaataaataa aatcttagaa tcttatctaa attattctta catgatttta
    14401 tttactttct attatctttc tcactttgga tattatcatc ttaatttatt tattactata
    14461 tcaatttact tattatcatc tcatttgtta tcgtcttact taatttactt attgatatat
    14521 caattccact tattatcatc ttatttaact tacttattgc tatatcaatt cacttattat
    14581 ctcatttgtt atcatcttac ttaacttact tatttctata tcaattcact tattatcgtc
    14641 ttactcaatt tacttattgc tatgtcaatt cacttattat tatctcattt gttatcatct
    14701 tacttaattt acttatttct atatcaattc acttattatc atcttaattt atttgcttat
    14761 tatcatttac tttattcaat ttctatatca aaaattagta tttgtactta atacttaaaa
    14821 ttaatttgaa gtctttacat attatttatt ttttattgta atattcattt ccatactttg
    14881 ctaagctact tccactcatt tattttaaaa tttcaattta ctatttctca attctatatt
    14941 attatttttc aattttatat tactaatttt aaagcttttt agctattatt cctacaatat
    15001 ttaaacaatt ttttaataat aaagctatat tacaatatat tcaaaatatg taatatttta
    15061 gatatttttc gttgacaatg ttaaattttt gttttatcct taattaagga catgttattg
    15121 acatttctga aacttatttg aaatagtttt ttcattttat ttaggaaaac gtattatact
    15181 taagaaaacg tattttctca tttcagataa gtaaaatatt tattcttttg aggaggaaat
    15241 catggaaatt aataaaaaac attttattca agtattttta acttgcttaa tcttggtagt
    15301 aatatgtgaa tttatagggc aaagaactat agctattgga aaagcctcta tagtactgtt
    15361 ccctatgctt tatgcagtta taattggact tataataaca ccagatttat taggtaaaaa
    15421 aattaaagca cttaaaaaag tggttggaga aaaagaaata aatattgctg gtgaagttgt
    15481 tggaattgca ctagtaatgc ttggtataaa atatggtact acagttggac caaacattga
    15541 caaaataatt caagctggac cagcgtttat cgctcaagaa tttggtcata tacttgcacc
    15601 aatcgttgca ttacctcttg cattaatgtt tggaatgaaa agagaagctg ttggagctac
    15661 tgcaagtata agtcgtgaac cttctttagg agttatatca gaaaaatatg ggataaactc
    15721 accagaaggt agtggtgtac ttggtacata tttaatggga acagttcttg gtactatata
    15781 ctttagtgta ctaggttcaa tatctattta tagtggtctt cacccatatg ctttgggaat
    15841 ggcctgtggt gttggtagtg gaagtatgat gacagcagct gctggttcat tatctaccat
    15901 ggtaccacct gaaatggcag atacaatact tgcctatgct gcaacaagca atatgatgtc
    15961 tagtataaca ggaattatat tcttagtttt tgtatcctta ccatttacta attttatgta
    16021 taagatttta gaaccaaaat tttcgagaaa taaaaaaact aaagaaatta aatctacaga
    16081 aggagagtgc taataaatgt ttgctaattt aaatttaaag aaaagttcat atcaattatt
    16141 ggttatgtct gttattggag ttataattct tataggacaa agaattagcg ttggtacatc
    16201 tatagttaca gctcttcctg gtatggtgat gctaatatta gctgctatgg cagctatgat
    16261 tataaaagat ttatttccta aatcaatctt tccagctttt ggatttgcaa ctataatcgg
    16321 ccttttactt agtatgccat atagtccaac atctgaagta ttcttaacta acactaataa
    16381 tattaacttt atggctataa ctactccact actagctttt gcaggaattt ctgttggaaa
    16441 caaaatagaa gctttaaaag aaatgtcatg gaaaatagtt ataatttcac ttattgtatt
    16501 tacaactata ttctttgcat gtgcgagtat agctcatata gttttaagta ttcaaggtaa
    16561 aatataggag gaatttatta tgaataaaga acaattaaaa tctctagtaa taaaaactat
    16621 agatgaaaat agagaaaaaa tattaaaaat aggtcaagga atatacaaaa atccagaata
    16681 tggatataaa gaatttaaaa ctacagaagc tgtttcaaac ttttttgagc aagaattaaa
    16741 tttatctgta gaaaaaaata tagcagttac aggatgcaaa gcagatgcaa attctagcaa
    16801 gaaaggtcct catatatcta tacttggaga attggatggt atatcatgta aagaacataa
    16861 agatgctaat gaaataggtg cttctcatac ttgtggacat aatattcaaa tagctggaat
    16921 gttaggtgct gctgttggtt tagtaaaaag tggcgtttta gaacatttag atggtaaagt
    16981 atcttttatg gctactcctg ctgaagagtt tattgagttg gaatacagag agcaattaaa
    17041 aaaagaagga aaaataactt attttggtgg aaaacaagaa ttaataaaaa gaggttattt
    17101 tgatgatata gacatatcta tgatgtttca tgcttctgat ttaggagaag ataaggctct
    17161 agttggtcct gagagtaatg gatttgttgg aaaaaaggtt aaatttatag gtaaagaatc
    17221 tcatgctggt tctgctccac atgatggaat aaatgcttta aatgcagcaa tgcttgctat
    17281 aaacaacgtg aatgcactta gagaaacttt taaagagtct gaaagagtta gattccaccc
    17341 tatcatcaca aaaggtggag atatagtaaa tgttgttcct gctgatgttc atatggaatc
    17401 atatgttaga gctagaacta taaatggtat gattgatgct aatgaaagaa taaataaggc
    17461 tttgatggct ggtggaatgg cagttggagc tgatgttgag ataactgaaa ttcctggata
    17521 tcttccaata ttacgataca aaagtcttga taatttattt aaaaacaatc ttgaagattt
    17581 aggtctaaaa ggaaaagtta ttgatggtgg agattttaca ggctcttttg attttggaga
    17641 tgtgtctcat ataatgccta ctcttcaccc tatgattggt ggtgtaaaag gagctcttca
    17701 tacaagagat tttgaaatag ttgatgaaga tttagcttac ataattcctg ctaaagcaat
    17761 ggcacttaca gttgtagacc ttctttttga taatgctaag gatgctaaag atatattaga
    17821 tacctttact ccagttatga ctaaagaaga atatctaaca ttcttagaaa aacacgataa
    17881 aactttataa gtctattttt ataatagggt ggtatctact gtaaaagtta taccactctt
    17941 tgtttttgta acagtatact tcttttcttt tggtttttag attaaactaa aatatattat
    18001 cctccttaaa acttttagag ctacgttgaa atatcagtag aactatgcta tattctttct
    18061 aattaatata aatttctatt ctaattcaag gtggtgaaaa aatgtttaat gcaggaaatc
    18121 gaattataaa tacatggttg taccctattc aagatggctt tgtcttgatt gatactggat
    18181 atgaaaattg ttttaaacat ttcaaaaaac gaatttctaa attcaacata aaaatcaaag
    18241 atattcgata catatttcta actcatgctc atgatgacca tgttggtttt ctaaatcaga
    18301 ttctagacga atctcctaat acaaagattg ttatgcatga taaagcttta gaaattctat
    18361 ataaaggtca aaactctttt attggtggtt gtaccagccg tactgccttt ctcttttgcc
    18421 aaattatgaa actatttgga aaaggtgagc atagtttttc tccacttaaa caagaatttg
    18481 aaaaaaggtg tattcttgtg tctgacaaga atcgtaaaga aattgaaaaa gaactaaatg
    18541 gaaaaattat agatacacca ggacatactg ctgattcaat ttcactacta attgatgatg
    18601 gtgttttatt ttgtggagat tctgcaatga atggatttcc aagtactcac aaaattacca
    18661 tatttgcaga aagcaaacta gaatttattc aatcttggaa aacaatcatc aatacaaaac
    18721 ctaaaatgat ttatcctgga catggaaaac catttgaata cagtaaatta gaacaaaatc
    18781 taatctatgt aaataaaata aatttatatc ctctacataa ttcaaaagat attcacagat
    18841 ttttgtagaa aatacaaagt aaatttattt gattaattaa aaaatctaac actttactac
    18901 gctacttatt ttaataactt aatcttccac tagcttttca taaaattttg ttttaaaatt
    18961 aattttaaag tgcatatgtt atttaaatac attagataaa attcgtcatt aatctactta
    19021 aagcatattt aactaacacc actcaaccag aaaattcaga aaactgcatt gacacaggaa
    19081 aaagtttatt ttatactttt tttaggacat attgtggaca cgtcttaaat cgacaaacag
    19141 aatttaaatt gtaagattaa ccacaatact tattttttaa ttatattttt aaacattagg
    19201 gaggatatta tgaatgataa cgtagttaag aagaaaaact tttttgacag gacattaaac
    19261 agaatagaaa ctgttggtaa taaactacca gacccagtta caatattttt agggttatgt
    19321 gtacttttac ttatactatc tagtctagtt ggttctatgg gtatatcagt agttcatcct
    19381 ggtactggag aaactataac cgctgtaaat ttattgactg tagaacagct tcaaattctt
    19441 ttaggaaaca tagtcagcaa tttccaaggg tttgctcctt tgggtctagt tctagtaact
    19501 atgataggtg ctggtgtatg tgacaaaaca ggacttatga cagcaacaat caaagcatca
    19561 gtaagcaaga ttcctgaaac aagagtaact cttgttgtaa tgactatagg tatgcttgcc
    19621 aatatagctt cagatgcagg tacaatatta tttccaccat tagctgcact tgtatatctt
    19681 ggtgttggta gacatccact tataggttta ttttctggat atgcagcagt ctgtctagga
    19741 tttgccgcaa acattatgat aagtgtcaat gatatacttg ctgcaagttt tacagttcca
    19801 gcagctcaaa tgattgattc tggatttcaa gccaatgcaa caatgaactt attttttatg
    19861 atagcatcaa cttttgttct aatacttttg gcaacatggg taacagaaaa aattgtagct
    19921 cctagatttg gtaaatatga aggaaatgca gaactagatg ttgatggaag tcttagtgat
    19981 gtagaaaaga aagggcttaa aaaagctggt ataagtatat taatatatgt ggctattata
    20041 gtagctctta gtgtaatcgg taaaaaacca ttcttagctg acccagaaac aggagattta
    20101 ctatcaaaca atgctcctct tatgaaaggt atggtaccta taatagcact agcatttttc
    20161 atacctggtg ttgtatacgg aaaaactata ggtaaaataa agaaggataa ggatgtagtc
    20221 tctataatgg ctagtgcaat gagtgatatg ggtggatata taatactagc atttgctgct
    20281 tcacaatttt tgcaattatt cacaaatagt aatttaggtc tgatactagc tgtaaagggc
    20341 ggagaatttt taaaatcagc aggaataaat ggacctttat tgttgatagg atttattata
    20401 ttatcatgtt ttataaattt atttataggt agtgcatctg caaagtgggc tattcttgca
    20461 cctatatttg taccaatgtt tatgatggtt gggtacaatc cagcacttac tcagatggct
    20521 tatcgtatag gtgattgtat aacaaatcca cttagcccat tatttccgta cttccctatt
    20581 atactagcat tcacaagaaa atacgataaa gatgctggta tgggtactgt aattgctaat
    20641 atgttaccat actctatatc attcttaata gtgtggacta tactattaat attgtttatg
    20701 gtatttaata ttccactagg acctggaata cttccttcat atagcatgta atttatttaa
    20761 atctttctaa aaggagaaat aatttatgtt agataaaaat agacttataa ataattttat
    20821 ggatatggtt agaattgata gcccatctaa tcaagaatta gaaatgtcta agtggttggt
    20881 aaactactta aaagaaagaa atatagatgc cattatagat gatgcaggtg aaaaatatgg
    20941 tggcaataca ggtaatgtca ttgcctatat aaaaggtgaa gaaggaagta gaccactttg
    21001 tttatgtgca cacatggacc aagttcaacc ttgtttaggt gtaaaaccaa tactagatgg
    21061 taatgtagtt agaagtgatg gaactactac acttggtgca gatgataaag ctggaatagc
    21121 tgctatactt gaagctttag aacatgttat tacagaaaaa ataccacata gagatattta
    21181 tctttgtttt acaatctgtg aggaagccgg tatgcatggt gtcaaaaact ttaatcctga
    21241 caacttgcct tgtaaagata tggtgatttt agattctggt ggagctattg gttcgatagc
    21301 atacaaagct cctgctcaac aatctataaa aatctctttt catggaaaga aagcacatgc
    21361 tggtatagaa cctgaaaaag ggttaaatgc tatattagta gcctctcatg caataagcaa
    21421 catgcacata ggcagaatag attcattgac tacttcaaat ataggtaaaa tagaaggtgg
    21481 tggagctaca aacatagtaa ctgataaggt tacattgact gctgaaatta ggtctcatat
    21541 acctgaaact ttagagtatg agcttaatca tatggaaaaa tgttgtaagg atgctgcatc
    21601 taaatttaat actacttata cttttgaaca taatatgtct taccctagtt ttgaacttag
    21661 tagagatagt catgttttca agttaagtga agaagcaata agacaagttg gtattgttcc
    21721 aaatccaatg gtaattggtg gtggaagtga cgcaaatata ttagctaatc ttggatataa
    21781 ttgtgctata ttaagtctag gtatgtatga tgtacacaca gttaatgaat atgtaaatat
    21841 agacgaatta tatgatactg ccaaaatagt ttatcatatg attaaattat aaatatcata
    21901 atagaactct ttaaattata aaaataaaaa cctatgtatc ctcaacttta taagttatat
    21961 attagagttg ttttacatag gttttagtat ttttatataa taatttgttt taacattttt
    22021 cataataagc tttgatattt tatatagtaa tttgttctaa catttttcat aataagcttc
    22081 aatattttgc ataataattt attctagcat ttttaataat aggctctagt attttatatt
    22141 aataatttat tctagtattt tacataatta tttgttctaa tttttttcat aataagcttt
    22201 aatactttat atagtaattt gttgtagaat tttatacaat ataatgattt atacagtttt
    22261 ataaaatatc ttaaaacagt atttcagtaa tattttttgg tcttcctcca ccaatactag
    22321 tctcttttgc acatattcga cctaaacctg catttactat tttatttaat atacgtcttg
    22381 cacttctttc acttacattt agatactctg ctaactcttt tgaatcataa atcttacttt
    22441 ttctattctc atttatagca ataatttttg caactgactc acaactcatt ccagtctttt
    22501 tagatatttc gattaccttt tcatcagata gcatcaaaga ataatttaat tcattttcgg
    22561 aagctaaagg tcctcttatg ttttcatctt catctaccaa atatatacca atttcttttg
    22621 aatctatgga gtgtcttaat gctttatgtg cattattttc agcctgatac gcagttactc
    22681 cagtaccaat tcctatacta agagaaaagc ccatgtcttt tatctctctc tttagattga
    22741 atagcttatc ataattatac tcattatcag ctaccccttt atgaacaaat acaacataat
    22801 catttcttcc aagtaaaaat aaggaacctt gaatacttct tacatagttt actacaagct
    22861 tatccatatc tgattttttt atcatgtcag aataatagtt atctatcttc tctttataat
    22921 ctattaaact caatatttca acagcaattt gagaaaatct tgccttactt agagcatatt
    22981 cactctttac cttgtcataa ctttctttga ttaattgtat agtagcttgt aaacgaaata
    23041 caggatagcc tctttctttt aattcattat atactgcacc aaaaccagtc aacattatat
    23101 ccgtcttatt ttcttcaaat aattttatat gccattctac aagttcagat tctcctactt
    23161 ccaaagaaaa tggattacaa aatacactcg ttgctttaat gtcaaattct ttaagtgcat
    23221 cttccacaac atatctatca actacatcta tactgaattt atctaatttt atatttgcac
    23281 tttttattgc ccaaaatgcc ttcattatac tcgtcccact tctcggtaca aatgaatgtg
    23341 gcttacttat ctcatacttg gattttataa attcataaac tgcacatcct gtaaatataa
    23401 tagcatcaca ttcgtcttca catttagaaa taacttctat tgtatctact actttttctc
    23461 ttacatatat tttagtcttt aaatcactgt ctatctgttt taaatcatcc tttatcttaa
    23521 acccagagtc tgaaggacct ataataccca ctatcataaa cttcgctcct aatccataaa
    23581 tattatactt tttaaaaatt atatcctcac tattttctac ttctatttaa ttattttgaa
    23641 ctactccatc ctacctacat ttagaatgga ataattagct tatttttgat aaagtaactt
    23701 ctcaaaatca gcacttggaa taggcttaca ataataataa ccttgaatta ccacacaatc
    23761 taaactcact aaaaattcag cttgttcttt tgtctctaca ccttctgcca ctacatccat
    23821 atctaaatct tttgccatgg ctatagtgtg ctttaaaact attttttctc tgtctgtgat
    23881 tccttcctca ttaaagaaac cacaatctat ctttaatgta tcaatattaa tattttttaa
    23941 catatttaat gaagaatatg ctgaaccaaa gtcatccata tttaaaacaa taccatccat
    24001 tctaaggtcg gctattatct tagtaaataa ttttatattt ttaataaata aactttctgt
    24061 aagctctaat tctaaaaaag attttggtat actatattta tttattaaac ttaaaacaat
    24121 ttctttaaaa tgtggattat gcaaatgtaa tcttgataca tttacagaga taggaactgg
    24181 ctcttcacct aaatctatcc attttctaat caaagaacat acttgttccc atacatacat
    24241 atctaatctg ataataaacc cattcttctc aaatagtgaa ataaatttaa ttggaggtat
    24301 tatacctttt tctggatgaa cccatctaac caatgcttct gcaccaacaa ttttatttgc
    24361 attaagatta tacttaggtt gtaaatatac atggaattgc ccattttcaa gtgcgtattc
    24421 catttccctt tccatctgtt tttcttcaag tgcatcattt cttatagtat catcataaaa
    24481 tatatagtta ttgatacttt gtttctttac ttttttgtga gcatatcctg ccatttcaca
    24541 cgcatttgta attggaatat gattatccaa aattctataa accccaaaac atggtactaa
    24601 catatattca aatttttttc tatttaagct atttttaatc ttataaatta ccttatgaat
    24661 atctctatca gagtaactct cataaaaaat gcaaaataca tctgcataca tacgacaaca
    24721 tataccatta gaaccaatgt ttttttgaat caactctgca acatatatta aagctttatc
    24781 tcctcctcta actccataaa tgtcattaat taccttgaac ttatctatat ccatcattat
    24841 aattgcataa cgcttgtcag gatatctttt aagcattaaa cttatttgtt cacaaaaact
    24901 ttcataattg tatagtcctg ttaatgtatc tttttggttt ttatgtagtt gcataagata
    24961 atctatattt tttatagcaa ctaaaatatg tgtttgtttc tcacctaact tatttttact
    25021 aataatcttt aacatataaa ttaatgccca atagtacttt ttatctattc ctaaacgtct
    25081 atactcaaaa tttagttcta aatcgtcttg cttgctatta ttaagactat caagagattt
    25141 tagaaggtgg ttatcattaa ataatattat atcatcagga tgaattaaat gtttttttat
    25201 gtatgaaaaa gttttttcaa gattttcctt tataggaaaa ctaaaaatat tgctctctga
    25261 ttgataaatt ggagtaatta aattcgtatc aatgcaaata gataaaatta tatcatatgt
    25321 attataagct aatttttcat aagccttaga ataccctttt atcattaaat catcaaaata
    25381 aaccttatca actaactctt tatgtttaaa taaaatatta ttcgtcgaat cagctaagtc
    25441 actaattact ttcatttcat tatttctaag tttgttctta ttataatacc tcttcttatc
    25501 ttggtacatt ctctcatcag caactttaat tagcttatgt atatcaatat tatcgtcact
    25561 ccaaatactg cccactgcta cactatatat catctcatcc ttaaattctt cacttaacca
    25621 actcactaat tcttcaaata gttgttttga tatatcttca cataatacaa caaactcatc
    25681 tcctccaata cggtaaacat tataagaatg aaagtatttt tttattatta cacatgctga
    25741 cacaatagca gaatcgccat tagcatgtcc aaagttatca ttaatatctt ttaacccatt
    25801 tatatctaca aatacaactc ctatggattt aacttttcct ttttgaaaat tatctatata
    25861 cttaatatat ttatttctat tatatgcacc agtcaagaag tcatagtagc tcatataatt
    25921 taatttattt tctacttttc tttttttcat ctcagttaat aaaaaataag atagggatgt
    25981 caaaagagag gtatctccaa cagctttatg tggattatca actccaataa accctactac
    26041 tttatcatct tgttttagtg aagatgtcat taaactttca attttttgag gttttaaaat
    26101 ttcatattct attggctgtt cttcttttat attttctata tcatcaatta ttacaaaacc
    26161 tttttgttcg aataataaaa accaattctt taattcatta atatcaagat ttgttagtgc
    26221 ctgtttttgt tcatcaacac cttcattgca ccattcatat gtattagtta ctttttttct
    26281 atcttctgat acttcaaaaa catatgctct atcagcttta tgaaatttac caatattggt
    26341 taatacaaga tttacagcat cagaaaaatt agattctgtt ataagatgtt taatacatcc
    26401 aactaataac tcctttatct ctaatttttc tgctattgac ttacttatat cttctttttc
    26461 tgtaatatca agagctattt ctaatcgtgc atgtttgccc tcccatttaa ttaacttgtc
    26521 ctttaatata aaatgtctat ttgttcgagt attcgtattt tcccatatgt aatattcatc
    26581 ttcttttaaa atagagtttg tacaaaattc gcagggagaa tctttcccct gtaatgcttt
    26641 ataacatttt aaatgggata tatcattaag tcccattgcc tttttaccaa tttcattaat
    26701 atataaaagt tcatatgtat ctaaagaaga aacatataca atttcatcca actcatctat
    26761 taaatttatt acatccattt atatatatct ccttttattc attttccaga ttgaattttt
    26821 aaagctttta aaattttgct atatatttgg caatgatatt atacttcttt atgttttaaa
    26881 atagtacctt aatgaggttc tattttttag taaatagaat tattattgtg tcaatcgtac
    26941 aatagagttt tgtaatacct aatttaatat atttataatt aattatctca ttatgtgttt
    27001 ataattaatt ctcatataaa tgacaaatct ccacctcaat taagtattcc aatcatatat
    27061 gaagataata cttcattcat ttaaaaatat aaaactatta tatattaaaa tttcttcaat
    27121 aataaattat ctagattgtt actttgatat attatataat atcacaaagg ctgatataaa
    27181 aagacaaaaa gggcaatttt tttatatcaa aaaaaaatgt taaattgata ttatccatat
    27241 ataaattgat attatccata tagatgctat atttaacaat tctacttact tcccttacac
    27301 ttattagcat atagttatta agagtttgtt gcaaaacaac acttagcaca ctttaaaaag
    27361 tgtggtctaa taagtaatca acgaagaaga cataataaag cataaaatat gatatacttt
    27421 tccacgccat caaaactgag gattttatta ttaatatatg ctaaaatatg cttaataaat
    27481 taaattttaa tgggagaata gttatgaaaa tatttagttt cttgttgttt gtgataggaa
    27541 tatgtttatg tattgctgga tttataggac attacccagt atttgttggt acaacattga
    27601 gttttcttgc aattgcaatg atagctgtaa gacaagtcaa aactaagaaa aaaatgaaat
    27661 aggcaaatcc taactttaca agttaatatt acacaagtaa ttagtctaaa attggatact
    27721 ttttctatta cagaaataca tcatccactt tgtattttct taaaaaaata aaatagctat
    27781 gtaaattcaa gattagatag attaactctc tatccatttc attgtaaaat atacatagct
    27841 atttgtattc gatttaaaat acttagattt tcttacataa caaataattt ctatctgatt
    27901 attactcata ttcatttagt aatcactcag caactaatca tttagtatct attcatccgc
    27961 catacggtaa cctaccccaa cctcagtcaa aatatattct ggctgagcag gattcttttc
    28021 aattttcctt cttatacttg ccataaaaac tctaagcgac tgattttcac tttcatagta
    28081 atttccccaa actttatcta ttataaactt atgggttaag actcttcctg aatacttaga
    28141 catcaactca ataatcttat attcgattgg agttaaatgt atttcttcac cattaattga
    28201 cacaatatga ttttcataat ctatctttag gtttttaact tcaaaagact ttggagcatc
    28261 attatttata ttatcctgtt gagctttatt tctaagagcc acacgtactc tagccaaaag
    28321 ctctgttaca ctaaaaggtt tagttaaata atcgtctgct cctccatcta aagcttccac
    28381 cttatccctc tcatgctctc tcgctgaaac aacaattatt ttattagtct ttgcacatgc
    28441 cctaactcgt cctattacct caatcccatc aatatctggt agtcctaaat ccaaaattat
    28501 caaatctggg tcaagagaat gtataagtga tatagctgtt cctccattat ctgtctctac
    28561 acacctataa ttttgagttt caagagatac ttttataaat ttacaaattg gcttatcatc
    28621 ttctacaatt agaactaatg gctttatttc cacttgtctt tcccctcctc atcatacata
    28681 ggtatgctaa atctaaatgt agctcctcca gaaggattat taaaggcgac aatttctcca
    28741 ccatgagctt ctactataga tttacatatc tccaatccaa gaccaatacc tcttctttca
    28801 agatttctag acttattctt agtataaaac ctattaaaaa tattcccgat atcttcattt
    28861 ggtattcctt ttccattgtc tgcaacttca aatagtactt gatttgactc tttttttaca
    28921 gttagttcta tcttagaatt tttaggtgta tgccttatag cattatcaat tagatttact
    28981 aacacttgtt ctattaataa tacatccaca tgaacgatta ttatctcatc cggaatattt
    29041 gtttttatgt ctctactatt ggcaaacttt ttgactctta atatagattc agctattatt
    29101 tcatcaacaa cctctggtcg tttttcaata tccaattttc cttcatctat tctggtcata
    29161 ctaagtatat tctcaacaga atgagttagc caactcgtat cttcatatat atttttcaat
    29221 aattctactc tagtttcttt atctatcaca tcatcatttt caagtattgt tgaagatgaa
    29281 cctaatatac ttgtaagtgg agttcttaaa tcatgagaaa ttgacctaag caagtttcct
    29341 cttaatctct ctctctctgc ctcaagattt gcttttttat ttttctcaaa taaattttct
    29401 ctatcaattg ccaacgcaac ctgtgttgaa actgcttcta atagtatctt ttcattttct
    29461 gtaataatat ctatcttaaa acacgcaaca cctataacac caagaacatt tgattcttga
    29521 ccttttatag ggaaataata tgcactgctg tttttacaat attttgtccc aattccaact
    29581 gggttacctg tcctaaaagc ttcttttata gcttcctttt ctaatactga atggaatata
    29641 ttctcacttc catcattgtt acaaatataa atacttggct tacctagatt attattctca
    29701 tctgcaatag tcactattac agttctattt aatatattta ctaaattaga cccacaaaat
    29761 tcaataattt gatttttatt ttttgcttta agcaacgttt tgttattctc atagagcaat
    29821 cttatcattt tttctctctt atgagaaagt ctcacttctc tttttatcct agaagttaaa
    29881 gtacttgtta taattgctgc cattagcata atacaaaagg tcataggata atcttttcta
    29941 taagctaaaa aagtatataa tggatgcgta aagaaaaaat taaatgatag cactcctatt
    30001 actgaagaaa gtattccaaa aaaatatcca taggttatac ttgtagcaaa tagtacacct
    30061 aatatgtata ctagtataat attagattca ttaaaaccta ccttttcaaa aactactgat
    30121 attccagtag caattgtcat aaccaatata gtttttatga agtccttaat aattatcttt
    30181 ttactgagat agccatcttc atcataatca tactctttta agtcttcatg ttgtagtcgt
    30241 tcctttttca tttactacaa ttcctcctta ttgttactgt ttaaattggc tctattttat
    30301 ataatacaat tttatcataa taaattcaat caatacattt aattaatgta cttaaattac
    30361 taaaaggtgc tgtcttaaaa tgaaaataaa tctctatttc aaaacagcac ctatatattt
    30421 aaattactaa tttagttttt acatacttaa atcaatagtt ttattcaatt tcataaatta
    30481 taatacttca attatagaac atttctatcc aacaagcata aatataaagt gggcacatat
    30541 acctgcacat aaaccaccta tagtatgcga aagtatcgcc ttaccagcta atggtcttgc
    30601 atctaaagca tccatcatac ctatgtgtgt acttaaatat ccactccaac acatacccat
    30661 tgctgtgaat actgctatat cattaggagt tatagcgcct gattttataa actctggtac
    30721 taaagatatt gctgctccaa cagctcctaa tgcagttaca gggaatgcta ttgcctctgg
    30781 tgatgtgaat ccaaataatg gttctattat gaagctaatc ttatcaccaa tagcaggtaa
    30841 taattttata ccttcatatg cagctcctgt gtaaacagct tgacctgtag cagggtctgt
    30901 agatggtcca aaagttaata acataactaa tgtacaaact actaaaactc ctggtattat
    30961 agccataccc atttcaacac ctaacttacc accttcaagt atagcatcca atgttctttg
    31021 gaatacatta ccatctctta tttctctaaa ctcttcaagt tttgcacctt tatcatttat
    31081 ttgcttttca gtatcatttt tagggtcata attataatac tttttagtaa aagtcaacat
    31141 aattctaaca ctaataatac tacctattat tgccccaaca ttacctatta tagctggtag
    31201 tacatattct ttaccttgag ctatcataaa agttgttaca ataagtccca taccaaaggc
    31261 tgttcctaaa ttacataaag caggtacttg atacttctta aaatattgtg taaatgtttt
    31321 gtctttagca aatggtataa tagctggatt atcagataaa tatgttgcaa ctactcctgc
    31381 tatactagca ccaggtaagc cccatatagg tctcataaga cccttaaata tcttattaac
    31441 taaagatatt actccaaact ctgaaagcaa tgcactaagt gcacctgcaa gaactgccat
    31501 agccattatt aaaaatactg tgtctaataa aagcgcatgt gctgtactca ttattacttt
    31561 aaacatcatt cctgcaccca tgatgcttcc tacatatcca aatcctaccg ccaatagtac
    31621 aataaatacg aaggtttcca tacttactgc cttgactctt ctcttatccg acagcttttc
    31681 catcttgaaa tcttccccct tttaatttta aattaaggtt cctaacattg gtacaaattt
    31741 tttggtacaa actgagttct aaaacttaca ctcgtaatgc aagttgtcag ttttttgaaa
    31801 taaaaaaaaa ttacgtttat agaataagaa tatcctcttt aatttggata ctcttattct
    31861 attttatgtt attatattat catccttttg tttaaaagtc aattttttct atttttgttg
    31921 tttaatttta tttatactct ttttatattt ttcattttct tttatctttg taagtactcc
    31981 atataagttg acttttctaa atacagtagt tataaatacc aatcctaatg caattgaact
    32041 tgcacacgat aatgtactca ccccatattc tacagctttt atataattat tggtcaaaac
    32101 agaatacata gtattatata ttccataacc aggtactaat attattaaac agcaaacact
    32161 taatgtagtt gctggagtca tataaattct tgcacataac tcacagtata tcgcaaaaca
    32221 aactgtagct ataaaaaaac ataagttttc tgttactccc atatttaaaa gaaatttata
    32281 cgaaaaccaa gctatacttc cacctattcc tgctagtatt aagttttttc ctttcatatt
    32341 aaatagcact ccaaagctta aggcagtaaa aaaagctgct attacttcta taaacaaatg
    32401 cacttatatc ccccctaact tgaagtacaa tccaagtacc aagaacattc ctactgctat
    32461 agatacacct acaaaaaata cttctgctaa ttttgcaaga ccagaaatca actgtccatc
    32521 taacaaatct cttaatgcat tagtcaaagc aagcccagga actaagagca ttattgctcc
    32581 tgctattaaa tggtctaacg tctttatgaa tccgagcttt aaaattaaaa ttgaacaaga
    32641 agctataata actgctccaa ttgcatttac aaaaaagtca ttaagggaag atttacttaa
    32701 atacataata acaaacttag tcagaatccc aacagtaaat gcagctataa attcttccac
    32761 atttcctcca aataatatcg aaaaagttgc tgctgcaata cctgcaaaaa atattgttat
    32821 taaatctgag taatagttat cttcacttat ttcatctaaa attttctcac ataattcaat
    32881 atctaaatta tttatactta ttgctcttga taatgagtta acttcatgaa ccaagtttag
    32941 gtctactctt cttgagctta ttcttttaat tgctgaatgt aatttcccat ctacaaatac
    33001 agatgccatt acagctgttg gagttgcaaa tacatctacg tggtctattc caaaagattg
    33061 acatattcta gctatcgttt cttcaactct ataggtttct cctccacttt gcaacatagc
    33121 tttacctgcg tttgaggaaa agtttaaaat tctatctata tccacaaaac atatctccca
    33181 ttcttttata gtattattca tattttaata caagatatta tttatttaaa ctattttaaa
    33241 taattcatat ccaatattta cattttatga aaaaacttgt tttttatgct tgtcaaaatc
    33301 aaattttaag cttgtttaca ccataaaatg gctgaattaa tatttgataa acactttcaa
    33361 ccatttatat aaccttctaa attgtataac tttttactac aaataaaatt atatctcttt
    33421 taattctttc tcttgagtat ttagcttata gcaacttcat atttatatat gtacaatata
    33481 aaacattatt tttatga
//
LOCUS       DAEMHZ010000034        33492 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000034 DAEMHZ010000000
VERSION     DAEMHZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.034"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      118..208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            616..933
                     /gene="flgB"
                     /locus_tag="KPK72_001456"
     CDS             616..933
                     /gene="flgB"
                     /locus_tag="KPK72_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG2488686.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            941..1345
                     /gene="flgC"
                     /locus_tag="KPK72_001457"
     CDS             941..1345
                     /gene="flgC"
                     /locus_tag="KPK72_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG2488687.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1355..1672
                     /gene="fliE"
                     /locus_tag="KPK72_001458"
     CDS             1355..1672
                     /gene="fliE"
                     /locus_tag="KPK72_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG2488688.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1697..3247
                     /gene="fliF"
                     /locus_tag="KPK72_001459"
     CDS             1697..3247
                     /gene="fliF"
                     /locus_tag="KPK72_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG2488689.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3260..4330
                     /gene="fliG"
                     /locus_tag="KPK72_001460"
     CDS             3260..4330
                     /gene="fliG"
                     /locus_tag="KPK72_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG2488690.1"
                     /translation="MSTEITNVNAFDLSDISESDKNEKIEGEGLKKAAVLLMTLGPDV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAIRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDAISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIVNS
                     VRKLEREGAITIVRNFGDDFVE"
     gene            4314..5054
                     /locus_tag="KPK72_001461"
     CDS             4314..5054
                     /locus_tag="KPK72_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG2488691.1"
                     /translation="MTLLNNKIIKSKKASYKGVLDLSIIDKVRVGESNLNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIKKEFKLFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            5058..6377
                     /gene="fliI"
                     /locus_tag="KPK72_001462"
     CDS             5058..6377
                     /gene="fliI"
                     /locus_tag="KPK72_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG2488692.1"
                     /translation="MYKTDIYIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6408..6854
                     /gene="fliJ"
                     /locus_tag="KPK72_001463"
     CDS             6408..6854
                     /gene="fliJ"
                     /locus_tag="KPK72_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG2488693.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6883..8088
                     /locus_tag="KPK72_001464"
     CDS             6883..8088
                     /locus_tag="KPK72_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG2488694.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFNSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENII
                     DDDRVDLLA"
     gene            8106..8714
                     /locus_tag="KPK72_001465"
     CDS             8106..8714
                     /locus_tag="KPK72_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG2488695.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEQESPEEA"
     gene            8741..9724
                     /locus_tag="KPK72_001466"
     CDS             8741..9724
                     /locus_tag="KPK72_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG2488696.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQIYAFQNPAGLDAAGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9737..9940
                     /locus_tag="KPK72_001467"
     CDS             9737..9940
                     /locus_tag="KPK72_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG2488697.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9940..10758
                     /locus_tag="KPK72_001468"
     CDS             9940..10758
                     /locus_tag="KPK72_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG2488698.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10748..11443
                     /locus_tag="KPK72_001469"
     CDS             10748..11443
                     /locus_tag="KPK72_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2488699.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11455..11898
                     /locus_tag="KPK72_001470"
     CDS             11455..11898
                     /locus_tag="KPK72_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG2488700.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11913..12287
                     /locus_tag="KPK72_001471"
     CDS             11913..12287
                     /locus_tag="KPK72_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG2488701.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEVLKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12288..12953
                     /gene="fliP"
                     /locus_tag="KPK72_001472"
     CDS             12288..12953
                     /gene="fliP"
                     /locus_tag="KPK72_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG2488702.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12966..13235
                     /gene="fliQ"
                     /locus_tag="KPK72_001473"
     CDS             12966..13235
                     /gene="fliQ"
                     /locus_tag="KPK72_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG2488703.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13247..15061
                     /locus_tag="KPK72_001474"
     CDS             13247..15061
                     /locus_tag="KPK72_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG2488704.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15070..17145
                     /gene="flhA"
                     /locus_tag="KPK72_001475"
     CDS             15070..17145
                     /gene="flhA"
                     /locus_tag="KPK72_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG2488705.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17120..18136
                     /gene="flhF"
                     /locus_tag="KPK72_001476"
     CDS             17120..18136
                     /gene="flhF"
                     /locus_tag="KPK72_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG2488706.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKKPMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18133..19011
                     /locus_tag="KPK72_001477"
     CDS             18133..19011
                     /locus_tag="KPK72_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG2488707.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19025..19726
                     /locus_tag="KPK72_001478"
     CDS             19025..19726
                     /locus_tag="KPK72_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG2488708.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19742..20164
                     /locus_tag="KPK72_001479"
     CDS             19742..20164
                     /locus_tag="KPK72_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2488709.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRSNSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20149..20334
                     /locus_tag="KPK72_001480"
     CDS             20149..20334
                     /locus_tag="KPK72_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2488710.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20375..21148
                     /gene="flgG"
                     /locus_tag="KPK72_001481"
     CDS             20375..21148
                     /gene="flgG"
                     /locus_tag="KPK72_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2488711.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21169..21930
                     /gene="flgG"
                     /locus_tag="KPK72_001482"
     CDS             21169..21930
                     /gene="flgG"
                     /locus_tag="KPK72_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2488712.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21953..22834
                     /locus_tag="KPK72_001483"
     CDS             21953..22834
                     /locus_tag="KPK72_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2488713.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22837..23211
                     /locus_tag="KPK72_001484"
     CDS             22837..23211
                     /locus_tag="KPK72_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2488714.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23243..24646
                     /locus_tag="KPK72_001485"
     CDS             23243..24646
                     /locus_tag="KPK72_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2488715.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKENDVKSKKNTVKN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKPSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24762..26699
                     /gene="htpG"
                     /locus_tag="KPK72_001486"
     CDS             24762..26699
                     /gene="htpG"
                     /locus_tag="KPK72_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG2488716.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27086..28216
                     /locus_tag="KPK72_001487"
     CDS             27086..28216
                     /locus_tag="KPK72_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2488717.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKVDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28443..29465
                     /gene="splB"
                     /locus_tag="KPK72_001488"
     CDS             28443..29465
                     /gene="splB"
                     /locus_tag="KPK72_001488"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG2488718.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29647..30558
                     /locus_tag="KPK72_001489"
     CDS             29647..30558
                     /locus_tag="KPK72_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2488719.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30573..30989
                     /locus_tag="KPK72_001490"
     CDS             30573..30989
                     /locus_tag="KPK72_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2488720.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSENNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31089..31481)
                     /locus_tag="KPK72_001491"
     CDS             complement(31089..31481)
                     /locus_tag="KPK72_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488721.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31622..32017
                     /locus_tag="KPK72_001492"
     CDS             31622..32017
                     /locus_tag="KPK72_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2488722.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            32272..32478
                     /locus_tag="KPK72_001493"
     CDS             32272..32478
                     /locus_tag="KPK72_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488723.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(32635..33366)
                     /locus_tag="KPK72_001494"
     CDS             complement(32635..33366)
                     /locus_tag="KPK72_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488724.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
BASE COUNT        13664 a         3566 c         6041 g        10221 t
ORIGIN      
        1 tataaatata ttaaaaatat tataaaattt aatattagaa attaaattaa aaaattatat
       61 aaaaaaatac ttaaaaaaat atttaaaaaa tttgttattt attgtatact taagttaaaa
      121 ctaaataggc aaatctagag aaatctagtg acgcaaagct atagggacta aggtttatat
      181 acataaacta tgtcagccag ttgccaaaaa gagtcctagg tgtattgtat acctaagaaa
      241 agtctataat gactgccttt ttggcagtca ttttgttatg gaaaaatcac tttatttcag
      301 tagaaaatac atataataac atattttacc aattataaca taaaataaag aaaaattatc
      361 tcaaaaagtt actattttac aaaaaaaatg acttctttat ttatttaaat taatcgcatt
      421 tccattacat taatacaata atgtatcaat ttagtaagaa aactaatatt tttttaatga
      481 agtcccatga aaatataaaa aaattttaat atgtatttaa atatttcttc gaaaagttgc
      541 tctttttgta atatagcaac ttcatttttt attaataaac aaaatagata aaatatattt
      601 tgaggagggg tgagtgtgaa tacatatgac attataaaaa tgggattaga tgcaactaat
      661 ctaagaagtc agacaatagc aaataatata gcaaatataa atactgaagg atataaaaga
      721 cattatataa cttttgaaga aacactaaat gatgctatgc ctaaaataga agaaaaaaca
      781 gacaattcta aaggtttaag agtagatggt aataatgtag acatagaaga agaaaaagta
      841 aatcaagcaa tggcaacatt acaatataat gctttgataa gttttgcaaa tggaaaaata
      901 gcaatgacca aatcaataat aagtgggagg taattagaag atgagtgtgt tcagtggaat
      961 gagaatatct gctagtggat taacagcaga aagtttgaaa atggatatca tatcatccaa
     1021 tgtagctaat ataaatgcta caagaggtcc aaatggagaa gcatatagga gaaaagttgt
     1081 ttcatttgaa gaaaactatg ataataaatt aggtatgcta ggagtaaaag ttacatctgt
     1141 tgaagatgat aaatcacctc ttaaagaagt atatgaccca tctcatccag atgcaaagcc
     1201 aaatggatat gtagaatatc caaatgtaaa tatattggta gaaatgacag atttaataac
     1261 agcttcacga gcatatgatt ctaacgtaga tacattgaat gctcagaagt ctatgatatc
     1321 aaaagctttg gaaataggaa agtagggata aattatgcca attacacaaa ttaacaattt
     1381 aatgcaaggt gcaaagttag ataatttgat tggagaagca aagccattaa ctcttaataa
     1441 taaagaagaa aaaaagaatt ttgaagatgt aataatagat actatagata aagttaattt
     1501 tcaacaagta acagcagatg atatgaaaga aaaatttata aaaggcgaag atgtttctat
     1561 gcatgaagtt atgttaatag gtcaagaagc acaactatct atgcaatatt taatagaagt
     1621 tagaaataag attcatgatg catatcaaga aatgaataag gtgcaaattt agccttttga
     1681 acaggagtaa taatcaatga taaccagttt taaaaataag attaaggagc aagttgctaa
     1741 atctaggaca aagataagtt ctttaaaaaa gggaactaaa atagcattag gtataggggt
     1801 tattgctata tgtttagcgc tagtatttac agcaatatct gcgagcaaaa ataagtatgc
     1861 agtcttattt tcagaactag atgctcaaga tgctgcagat atatcagcag agctagagtc
     1921 taaaaagata gatactaagg taaaaggaag tacgatttat gtacctagaa cagaagttga
     1981 taagttaagg ctggagttag cgccaagtct cacaggtggt tctaaaggat ttgcactaat
     2041 ggatgatagt tcatcaatag ggcttactga tgaagaattt cagattaaga aacaaagaat
     2101 gatacaaggt gaaatagaaa aaacaataaa gagttttcca caagtggaag acactagagt
     2161 acatataaca tttggagagt catcagtatt tgagaaggag gctgttccag ggagtgcagc
     2221 agtttatttg atgctaaaac cagggacgac tttagaaaaa aaccaaataa agtctgttat
     2281 ggcacttata tcaggaagcg ttacaaatat accagagaaa aatgttcagg ttattgataa
     2341 caaaatgaat ttattatctg aaggtatttt ggaggatgaa aatgttgatg aaaataatca
     2401 accaataaat agtggcatta gtgttgataa aaataaggca tctgaacaag cattgaataa
     2461 acaactagaa aagtctattt tagaaatgct agaaccgata tttggtaagg gaaaggtgaa
     2521 agctacagta aatgctgatt taagttttga tacagctgaa aaaactgaga taaaaattga
     2581 ccctgataaa gttgcaataa aggagacaag gtcgaaaaat tcatctaaat ctgctgcaaa
     2641 taatgttcaa ggtgtagatg ctaacatgaa taaccaagga aataacaatg gtactaatac
     2701 agaagatagt ttagatgaga gttttgagta tgaaacaggt aagattgaaa ctcacactat
     2761 agttgcgcct ggagaactta atagattgac agcttctgtt gcaatagatg gaaaaatagc
     2821 taaaggtgtt caagctgatg tagaggatat tgttaataat gctataggta tggatggagc
     2881 gaggggagat actcttagcg ttgtttcaat ggtatttgac ccagaaggta agaaagaggc
     2941 taaacaagag ctggaagaga ttaaaaaaga aggatttaaa aagaacataa taaaagcagt
     3001 tattggagta gtagcagtaa tagcagttgg agctattgga tacttaatat ataagaaaag
     3061 aagtgaacga gaagatatgg attatgaaga tgaagattat atttctaaat ctgattctgt
     3121 tgataggaaa gataatcctc tattaggaga tgacccagaa ctgacattag aggaagctgt
     3181 taaaatgtat gcagaagaaa agccagacca agtaacagag ataataaaaa cttggttaaa
     3241 tgagtaaggg ggatagaaag tgtccacaga aattacaaat gttaatgcgt ttgacttaag
     3301 tgatataagt gaaagtgata agaacgagaa aatagaaggt gaaggtttaa aaaaagctgc
     3361 tgtattatta atgacattag ggccagatgt ctctagcgat ataataaaaa gtttgcctga
     3421 taaacaagtt caaaaaatag gtttggaaat tgcaaatatt tcttcaatag cttctaaaga
     3481 aagaagagaa atcttaaaag agtttgtaga aataaataga gcaaaagatt atgttataga
     3541 aggtgggctt gaatatgcaa gagagttatt aagtaatgct ttgggaaatg ctaaagcaaa
     3601 caaattactt gaaggtatat caataaatac aagtacaaag ccttttaaac atattatcaa
     3661 actagatgta aagaagatat tagaaatact taggaatgaa agtgcacaaa ctatagccgt
     3721 tttacttgca catatggaac cagaaaatgg ggcagaagtt ttatcatatt tagactttga
     3781 tttacaaaat gaagtagcta ttagaataag ttcaatgggt tcaatatctc cagaagtaat
     3841 aaaggtaatt gaaagttcat tagaagataa attacattca ataagtggtg atgattttaa
     3901 ggattatgga ggagtgtcta cattagttca aatattgatt aattctgata gaaaaacaga
     3961 aaagaatatc ttgggaacta ttgaagaaca aaacccggat ttagcagctc aaattaaatc
     4021 tcatatattt gtctttgaag atattgtcaa gttagatgat ataagcatac aaaaagtgtt
     4081 aaaagaagta actatgaaag atttagtata tgccttaaaa ggtgtggatg atgcgatttc
     4141 agatgctata tatagaaatc aatcttctag ggcagcagag gcacttagag aagaaatgga
     4201 aatggttggt gttataaagg ttacacaggt tgaggctgct caacaaaaaa tagtaaatag
     4261 tgtaagaaaa cttgaaagag aaggtgccat aactattgta aggaattttg gtgatgactt
     4321 tgttgaataa taagattatt aaaagtaaaa aagcatcata taaaggagtt ttagatttat
     4381 ccataataga taaagttaga gtgggagaat caaatctaaa taaagaaaat gattgtagat
     4441 ttagggatga ggtaataaag gaagctatag aatctgcaga gataaaaaag aatgagatat
     4501 tagaagaagc aaaaatgaac gcacaaaata tagaaaaaca agcatatgaa aaagggtata
     4561 gtcaaggtca aaaaaatgga tatgaggatg gatataaaga gtcatatgat gaatataccc
     4621 aaaaagctaa agatgaagca gattttataa aaaatcaggc aaatttaatc ttattacaag
     4681 ctaatgagaa gatgactgag tacataaaag aaaaaaataa agagattgta agacttgcta
     4741 ttaatatggc cagttctgtt ttaaaaaagc atttcgaaga tgaagaatct atgagtgatt
     4801 tgttaaagca aattattcaa gattatgaag gaagtagttc atttatagta agatgtaatt
     4861 ctttctataa agataatata aagaaagaat ttaaattatt taaagaggaa acatcactaa
     4921 aatcaagtgt atttgttatt gcagatgata gtatagataa aggaaatgct attatccaaa
     4981 aggaaaatgg aagaataata gttggtattg attgtgctat ggaaaaacta aaagaagaat
     5041 tgcttggtga ataaagtatg tataaaactg atatatatat agattttgac aaaataaata
     5101 agaaaataga aaatttagaa ctcactatat cagaaggtag agtaaagaag ataataggac
     5161 ttactgtaga agttgaagga ataaaagctt ttgttgggga gttatgcgtt atatacaacc
     5221 aagttaataa accagtgaat tgtgaagtag taggttttaa agataaagaa gtcatattaa
     5281 tggcgttggg agaattaaca ttgatagcac ctggttgtaa agttatttct aaggggatac
     5341 ctttaagtgt tatgtgtagt gataatttac taggtaaagt acttgatggt cttggaaatc
     5401 ctatagacaa ctcagatgct gttttaggag atagatacaa cctgaacaat gaacctcctg
     5461 acccaatgaa gagaaaaaag atacgaaata taatggaaac aggtgtaaga gcaatagatg
     5521 catttactac ttgtggtgaa ggacagagaa tcggcatatt tgcaggaagt ggagtaggta
     5581 agagtacgac tctaggtatg attgctagga atgcaaaagc agatgtaaat gtaattgctc
     5641 ttattggaga aagaggaaga gaagtactag attttattga taaagattta ggtgaagagg
     5701 gaatgaaaaa atctgttgtt gtgtgtgcaa catcagataa agccccttta gttaggttaa
     5761 aaggtgctct tactgctact gcaatagcag aatattttag agaccaaggc aaaaaagtaa
     5821 tacttatgat ggattcagta actagatttg ctatggcaca aagagaagtt ggtgtagcaa
     5881 taggagagcc accagctcaa aaaggatata caccatctgt atttgcaatg ttgccaaaac
     5941 ttatggagag aacagggaca tcagataaag gttctataac tgcattttat acagtattag
     6001 ttgatggaga tgactttaat gagccaatag cagatacaac aagagggata cttgatggtc
     6061 atatagtttt atctagagac ttggcaaata agaatcacta tccttctata gatgtattaa
     6121 acagtttaag taggcttatg aatgagattg cttcaaaaga agacataaaa atagcgtctt
     6181 ttgcaagaga catgcttgct gagtatagag aagcagaaga tttgataaat ataggtgcat
     6241 atgcttcagg tacaaataaa aaaatagatg aagcaatcta ttatcatgag catattatta
     6301 attttttaaa acaagggata aatgaaaaga gttcttttaa tgaaactata agtagtttga
     6361 gaagagtttt tgaataaaca attaaaaaaa tatttgaata ggttgtgatg gaattggata
     6421 aaaactttaa gtttagatta cagaaagttt tagacttaaa agtgaaagat gaagaagaga
     6481 taaaaatgga atttgctaag attcaacaaa aaaaaataga tatagagact aatttagaaa
     6541 acttagaaag taattatagc aaatattcta taagtaaaaa taacgatagt attcaaaatc
     6601 aaaaaataac tattaactat ttactagctt taaacaattc tataatggat ttatcagaag
     6661 agttggataa gtcaacaaat gaacttgaaa aagctagaaa gcagcttata agcaaacaga
     6721 tagagagaaa atcattagaa aaattaaaag aaaaaaagta tggacagtat tataaagaag
     6781 aaaatttaaa agaacaaaac actaatgatg agtttgcaag tatgagttat ttaaggaata
     6841 gacaagttct ataagaatag taggaagtga ggatattatt gtatgaagat acaagcagat
     6901 tcttataata ttattaaaga tattggggat agaaaaagtt catctaagaa caaaaatata
     6961 aaaaataata aatttgaaga taatctatat aaggcaacag accataaaaa ggttaaagat
     7021 agaagggaag taagtaagca aaatattagc tcagataaac cggaaaatag tgataatgag
     7081 tgtcatttag atgaagataa aagtaatcac ttagaagatg aaaatacata tgaaaaagat
     7141 tgtagtaaga ataatgaagc tttgcagcaa ttactgaatc ttttaaagga taattataaa
     7201 aaagataaag atataaacac tatattgaat aatatagaaa aactaagtat atcagatgaa
     7261 ttaaaacaag aacttaagct taataaaaat atattaaata agattgcaaa tcaaaattca
     7321 gagctaaata aagctaatat aaataactct aatgatatag aaaatttaga catagttaaa
     7381 ttaagagaac ttttggaaga taatgctaat aaagatattg aagtaattac taaaactaca
     7441 gaaaatgata ctaaaaatat agatttgtat agttttaata gcaatagaat ggataatctt
     7501 aactcaaata aacagaaaga tgatagctct gatattttgg aaaaactagc aggggtaaat
     7561 ggaaataaga gtgggaattt tggacaagta ataaataaaa atgttgatat gaataaaatt
     7621 aaaaatgata atgtcaattt aaaacagata gaatcaaatg taatggatga ctctataaaa
     7681 gctattaagc atatgaaaac aaatgatata caagaattga caattaaatt aagaccaaaa
     7741 gaattaggag atatgaatat acaattgtta aaagatggtg aaagtatgag agctgttgtg
     7801 actgttttca ataaagatgt ctttgactct ataaataaaa acatagcaga tttaaagcaa
     7861 catcttgaac ttactaatgt aaatataaaa gatgtatcag tgcaaatgca cagcgataat
     7921 acaaatacat ctgatacctt tgataaagca tttgaacaac agaatagaca aaataaccaa
     7981 gaaaacatga gtaagaagaa tacaaatgat aagcataata atgtagtcat agaagatgac
     8041 attaaagaaa atattattga tgatgataga gtagatttac tagcttagta agggggaagt
     8101 attgcatgta cggaatagca acggctttgg caggaactca gggaaactat aggtcaaata
     8161 acttaagcca acttgaaaga aatattaatg atattgccaa taaacaagtt agaagcaaag
     8221 gaaatcagac agtaacagac aatgggactc caattatagg acaaggtgat gatgctgata
     8281 aagacttgtt tatgaagata cttgtggcac agatgggtaa tcaagaccca atgaatccgc
     8341 aagacccaac agaatatgtg tcacaattag cacagtttgc aactttagaa aagatgtcag
     8401 ctatgaatga gaagctagat gtacttttag ccttaagtaa ttccactcta ataaactctg
     8461 ctttgagtat ggcaacatca ttaattggta aagaagtaga agtatctggg gaagagaaag
     8521 aagatgaaaa cattgtagga gtagtaaagt ccacatatat aaaagatggg gaagtacact
     8581 tagaattaga agtagatgga agtgacaaaa ctgtaggtgt taaatatgaa aaattactta
     8641 aaataggaga ggtagatgaa aattcaggag aagaaaatga ctcttcagaa caagaatctc
     8701 cagaagaagc ataatattaa atgagaaaag aggtaaattc atgataaaag caatgtattc
     8761 aggtgtaagt ggaatgaaag caaatcaaac aaaattagat gtaataggta acaatgtagc
     8821 caacgctgga actacttctt ttaagaaaag ttcagctaga ataacagatt cattttatca
     8881 aactgtacta tatgcaagtg ctccaacagc tgcattagga ggaacaaact taggacaagt
     8941 aggtgtaggt agtaagatat caagtataaa taaagatatg gtacaaggaa atgtacaacc
     9001 aactaataga ccatctgatt taatgataga tggagatgga tatcttccag tagtaagaca
     9061 aggaacagtt atgtatacga gagatggttc ttttaattta gatacaggat ttaaagcagc
     9121 tgatggtgct ttgaagaata ttaaaggtgg aagacttgta aatggtgatg gatatatgtt
     9181 acaaggtatg atttatgatg gggaatataa agctgatact ggagaatttg ttaaaaaagg
     9241 tgatgctaaa ggaaaagagc ctattgtaat accacttcaa agtataagtc ctaaggatgg
     9301 aacagttcaa aatgtactag agtataatat atctaaggat ggaacagtag aatttttatt
     9361 gagtgatggt caaagaactc aatttgtcta tgatatacct gatggtgcta aggtaggaga
     9421 tacacctgct ggaatagata aattaaaagg tacaatacaa aaagtgcaaa tatatgcatt
     9481 ccaaaatcca gcaggcctgg atgctgctgg aggaaacttg tttaaaccat cagcaaactc
     9541 aggagaccca caaatagcag gagctacagg cacaataata caaggggcaa tagaagcttc
     9601 caatgtagat atagcagaag aatttacaga aatgataata gcaagtaggt ctttccaagc
     9661 aaacagtaaa ataatatcga catcagacga gatacttcaa gaaataataa atcttaagag
     9721 atagtaggta catattatga taagagtaac ggatttaaac ggaagaaaat acatattaaa
     9781 ttcagacctt atagtgagga ttgattctgt accagaaagt aggattctcc ttacaacagg
     9841 agaaaaaaac cttgtaaaag agtcggtaga tgagatagta gataaagtaa taaagtataa
     9901 aagaaaaata aatgtaggtt atattaggag cgaaaaataa tgaaaaaaac agattcactg
     9961 acatttattg gactaatagt atctacagta ttagttttag ttggtgccgc aaaaggaagt
    10021 agttcagggt taaagaattt ttttgatgtt tcatcaatat taatcacagt gcttggttca
    10081 tttggagctc taatgattac atttactata gatgatataa aacttataaa aaatgcatta
    10141 caatactcat ttaagacaat gagtgtatca aagttagatt tactagaaca atttaaaaca
    10201 ctttctaaaa aagctagaaa agaaggtctt ttatcaatag agagtgatgt aatgggaata
    10261 gaagatccat ttatgaagaa agggttagag ctgtgtattg atggattgga aatcaatgag
    10321 ataaggtcca tcttagaact agagatagat tctgttgaag atacaaacaa cagatactct
    10381 aaactattta aaacttgggg aacctttgca cctgcttttg ggatgttggg aacactaata
    10441 ggtttaatac aaatgttagc tgaccttaca tctccagatt taatagcatc tggtatggga
    10501 aaggcattaa ttacaacgtt ttatggttct ttacttgcca atatagcttt gaacccaatt
    10561 gcatataata tagatgagaa gactgaaaaa gaaatttatg taaaggaaat gatgttggaa
    10621 ggtataataa gcatacaatc tggggagagc agtatagtag ttgaggaacg tttagccaca
    10681 tacctttcaa acaatgagaa gttagaaatt atgaaatcca ataaaaatac agaaagagca
    10741 atgagcaatg gcgcgtaaaa aaaataagaa aaaggatgag ataaatccag atgcatggct
    10801 ggctacttat gcagatacta taacactgat tcttacattt ttcgtattat tgtatgcaac
    10861 atcagttgta gatatggaaa aatttaaaaa catagcagat gcgttaaaag gtcagttttc
    10921 tgggacaagt atatttagta cgggttcaag tacaagtgag aaatctccaa tagacagtgt
    10981 tttagtggat aaaaatgata aatcaaattt agaacaggat ttaaaagata aagttaataa
    11041 ttctaactta caaaatagtg taacagtcaa agatgatagt agagggattt tactagagtt
    11101 agatgattca attttatttg actctggcat ttctgagtta aaagtagata gtaaagctac
    11161 tcttaataaa gtatatgaca tgataaagat gatgaaaaat aagatagtta tagaaggcca
    11221 tactgataat gtacctgtaa aatcaagtac acatgaatcc aattgggatt tatcttctag
    11281 tagagcagta agtgttgtaa gatactttgt agaagaaaag ggcatggacc ctagactatt
    11341 ttcggcaaca ggatgtggag agtatcaacc tttggtttca aatgaaacag caaagggaag
    11401 ggctgagaat agaagagtta atatacttat aactattgac taggaggaag aggtttgttc
    11461 aaaaataaaa aaattattat aattattttg atactgatta ttttctcagc gggagcaggt
    11521 ttttttatat taggagaagg tggaaataaa caagttgaca agattgcagg tttgtttatt
    11581 ccaacaggaa aaggtaaaga tgaagtaaag accgaaaatc ttgacttaga agagatggta
    11641 ttaaagctag ctgatgaagg tgtaagatat ttaaaagtat caataactgt atcttatgat
    11701 tcttcatata aagatataga aaaaaatacg cctttaatta gggataatat tataaattgt
    11761 tttatggcaa aaagggcaga tgattttact gctgataatt taaagaatat aaaagaagaa
    11821 attaaaaata cattaaatat acaactaggt gaaaatgtaa ttcaaaatat atatttcaca
    11881 aatatagtag tgcaataaaa ggatggtaag acatgatact ttatttacta aaaatattaa
    11941 tatgtatccc aataatttta atattgatag tcttatcttt aaaaattagc caaggaactt
    12001 tattaaaaaa atataatgat aaatatgtaa aaatactaga tattgtaaat ataagtaaaa
    12061 acaatagcat aataatttta aaaataggtg aagaaggatg cattatggca tcttcttctt
    12121 ctagcatgag taaaataaaa gatttgacca aagaagaagt attaaaaata gaaaacgaca
    12181 ttaaagaaag aaattttaaa attcaagatt ttaaaatgga aagaattaaa aagaaatatg
    12241 atttcagtag atatattgga aaatttacaa aaaaggaaga taagtaaatg gaactattat
    12301 caaaattacc tttggctgat gtaccatata caagttcaat gcaattgttt ttatttctaa
    12361 ctgcattaat gtttttacca tttgtaatgt tcatgatgac aagctttgtc agaatagtta
    12421 taagtttatc atttttgaaa tcagcattag gagcacagca agcaattcca agtcaaattt
    12481 tggtaggact tgcaatagta ttgacaatat ttataatgag accagtatta aatgagataa
    12541 acgaaaaggc attacaacca tatatgaaag aagaagttac tatggaagaa gctatgaagg
    12601 aagcagaagg acctattaaa gagtttttat taacacaaac cagacagaca gatttagatt
    12661 tatttgtaga acaggctggt ttaaaagaaa agaaattaac tcgtgaaaat ataccactat
    12721 ctgttgtagt tccagcattt gctataagcg aactaaaaac tgcttttcaa ataggttttt
    12781 taatatatat accatttttg ataatagacc ttgttgtggc aagtgttttg atgtctatgg
    12841 gtatgtttat gttgcctcca gtcatgatat cattaccatt taagttatta ttatttgtaa
    12901 tggtggatgg atggaacttg atagtaaaaa cattgatatt gggatttgga taggagaaaa
    12961 atagtatgag tgaatttatg ataatgagtg ttcttaaaaa tggcatgttt acaggagcta
    13021 tgattgcagc tccagtactc attgtttctc ttgtagttgg gcttttaata agtgtgtttc
    13081 aagctacaac tcaaatccag gagcaaactt tgacttttgt accaaaacta atagctattc
    13141 ttttagtagt agctattggt ggaagctgga tgttacatac tcttattagt tttacttctg
    13201 agatattcaa tatgatatct cttataacta aataagtagg atacatatga tttttgtatt
    13261 tattagaata gtagcattct ttggaacact aagaatacta tttccagctg gtacacctgc
    13321 tttatttaag tcactctttg caatcattat atcaatattg atttcaagta ctatgcaaat
    13381 tgaatatgca acaaatatag acaacattgt attgtttacc ttatatggtg taaatgagac
    13441 cataactggg attatgcttg gatatataac caacctatgt ttttatagta tcagaatggc
    13501 gggttctatg atggaccaac agatgggatt atcaatgatt aatatgtttg acccaaattc
    13561 aatgactcag acaactttaa ttgataatct tatgaactgg acagcactta tgatattttt
    13621 tagcatggat ggtcatcatg ttttaattag aggtataagg tatagttttg agttaattcc
    13681 aataggaaaa ccatttgtag ataataacat agattatata ataaacatat ttgtgcagtg
    13741 ctttttaact ggattcaaaa tagcaatacc tattgtactt tgtctgctta tggcagattt
    13801 tattttaggg cttatatcaa gaagtgtacc tcaattaaat gtaatgatag ttggtatgcc
    13861 attaaaaata ttggttggta ttgcattatt tatcatatca atacctttaa ttgtcaatca
    13921 aataagtcat ttattaagtc aaataccaaa gatgtatgag ggaacttttg cactagctcc
    13981 tatgtttttt atgggttcag cagataaaac agaagaagct actccaaaga aaaagggaga
    14041 acaaagaaag aaaggtaata tagcaaagag tagagaatta cccgttgcaa tgactctact
    14101 tgcattcacc ttattagtac ctacattatt ttcatatgta gtagatacac ttaaatcttc
    14161 gcttaattat tttttgagtc tagattttta tatgaacata aattattcaa acctagagaa
    14221 attagttata gcaggattaa tggatttttt taagatattt ttaccaatag ctattccctt
    14281 tttagtttta ggtataatag caaacctatt tcaggtggga atactgttta ctggtgaaac
    14341 tttaaaacca aatttatcaa aattaaatcc aattagtgga ttcaaaaaca tgttttctat
    14401 gagaagtttg tcaaccttaa taaaggatac agcgattata tctattttgg cttacatagg
    14461 atatacattc ttccaagata attatttaga tatattaaag ttaggtaaca tatacctacc
    14521 aactttaatg tacacagtta aagacttagt atattcaata ttgagtaaga tttgtgtagc
    14581 tatgatagct atagcagttg cagattatgt ttatcaaaga tacagtcata aaaagcaact
    14641 gagaatgaca aaacaagaag ttaaagatga atataaaaac tcagaaggag acccagaagt
    14701 aaaagctaag ataaaacaaa aacaaagaca aatttcgtct caaaggacaa tgcaagcagt
    14761 accaagtgct actgtaatag taaccaatcc aacccactta tctatagcag tgagatatga
    14821 aaaagggaaa gaccaagctc cagtagtggt ggcaaaaggt gcagattatt tagcttttaa
    14881 aataagggaa attgcaaagg gaaatgacat acctataata gaaaacaaac ctattgctag
    14941 gttgttatat aagcaagtag aaatagacca agaaattcca gaggacatgt atcaagcttt
    15001 tgcagagata ttagtggcag tatataaaat caaaaataga tacaaagtcc ccaaaaggta
    15061 ggataattaa tgaataagtt taatttaaag gaaaatatgg atgtaatagt ggcatttggt
    15121 gttgtaggga ttgttcttat gatgatagtg ccattaccta catttatatt agatatttta
    15181 ctggcaatta acataagttt atctgtgcta attttactaa tgacattatt tacaacaaat
    15241 gtacttgatt tatctatatt tccaacagtg ttacttgtga caacactatt tagattggga
    15301 ttaaatcttt catcaactag attgatttta acacagggat atgcaggtga agttatagag
    15361 gcatttggaa gttttgttgt aggtggaaat tacgtagtag gaataattat attcttaatt
    15421 atattaattg ttcaatttgt tgttataacc aatggttctg gaagagtatc tgaagtaaca
    15481 gctagattta cgttagatgc catgccaggt aagcagatga gtatagatgc agatttaaat
    15541 gcaggagtta tagatgacaa aactgctaga aaaagaagaa aagagcttca acaagaagca
    15601 gatttttatg gttctatgga tggagcaaat aagtttgtaa agggagatgc catagcaggt
    15661 cttatagtaa cagcaataaa cataataggt ggaattgtaa taggagctgt aatgctgaat
    15721 atgactctta tgggtgcagc acagacatat gtaagactta ctatagggga tggtcttgta
    15781 agtcagatac ctgctctatt aatatctaca tcagcaggta taatcgtaac aagagcttct
    15841 actgatgaaa actttggtgg attagtaggt aaacagttaa cggcaatacc aaaagcagta
    15901 atgatgactg gttgtgttct aatcgtactt ggtatgatgc ctggattacc taaaccagca
    15961 tttttttcac taggtatagg agctatagca gcaggttact ttttaggaag agaaaagaaa
    16021 gatactttag aagatttgga gtttgaaaaa cctgatatgg taaacccaga taaaaatgaa
    16081 gtagaagatg taacaaattt aataaatgta gaatcaatag aagtagaaat tggatatggt
    16141 ttaatatctt tagctgatga atcagcagga ggagatttac ttcagagaat agcatcaata
    16201 agaagacaat gtgcaataga catgggtata gtcgtacaac ccataagaat aagagataac
    16261 ttacagttaa atccaaacca atacaatata aaaataaaag gaaatgtagt tggtgggtat
    16321 gagataatgc ctaatatggt tttatgtatg gatcctatga atcaaggatt tagtatattt
    16381 ggaataaaga caattgaacc tacttttgga atagaggcac tttggataga aagtgacaag
    16441 gtagaagatg cagagttaaa gggttataca gttgtagatt catccacaat acttataact
    16501 cacttactag atataataaa atcaaatgca cacgaattac tgggaagaca agaagttaag
    16561 actattatag atgcagcaag agaaaattat agtgctgtag tagatgaatt gataccagat
    16621 ttgatgacac ttggtgaaat tcaaaaagta cttaaaaatc tgttaagaga aaaagtaaat
    16681 ataaaggata gagttacaat acttgaaacg cttgcagacc agtctagaaa tacaaaagat
    16741 atagagcttc ttactgaata tgtaagaata gccttggcta gaagtatttg taataattta
    16801 gttgatgatg ataagacaat tgtagtggct acattgtctc cagaaactga agagttgata
    16861 tctaataatt tacagaggtc tgtaaatgga acttatccag cagttgaccc agaaaataca
    16921 aataaaatat ttgaaagtat acaagaagta atgagcaatg tatatttcaa tcataatata
    16981 ccagtaatag tagtatcacc aaagataaga gctccattta gaaaattagt tgaaattgta
    17041 tatcctaatt tgactgtatt atcattaaat gaaataccta acgatattaa aatcaaagca
    17101 gaaggtgtgg tgagcatata tgatgacgaa aaagtataca gctaacacaa ttcaggatgc
    17161 tatgaatcta gccaaaatgg aattgggtga caatattaca ttaatagata aaaaagaagt
    17221 aagaaagtct ggaatacaag gtatcttttc aaagaaagat attgaactaa ctattggatg
    17281 ggaaaagaga gaaaatgtag aacagaaaga tttaaaacga gagatagaac aattaaagtc
    17341 aattattaac aatatggggt ttgataataa gaatgataat gatatagata aaatatgtaa
    17401 aaatctacta agtttagaat tgaatgaaga aattgtagaa tctataagaa ttgatttgca
    17461 agaaatgaag ttcaatggaa tcgatacaag taaaaattta gtagaaattt taaagaaaaa
    17521 gattaaaata gaaaatcaag taataaatgg taaaattgct ctagtcggac caccaggagt
    17581 aggtaaaacg actacaatag caaagttagc cgcaaaatta gtttttgaag aaaataaaaa
    17641 agtaggagta ataactatag atacatacag gattggtgct gtagaacagt tgaagatata
    17701 tactgatata atgaatattc cctttaaagg ggtaataagt cctgatgaaa tggaattggc
    17761 acttgacgag atgaaagatt gtgatgttgt attaatagat acaacaggta gaggctacaa
    17821 aaagcctatg caaatactag aaatcaaaaa tcttatagat aaagctgaaa ctgataatat
    17881 acatttagtt ttgaattgta caacaagaga aagtgataca aaagcaatta tagattcata
    17941 tagaaatgtg aattttaaaa gcttaataat tacaaagcta gatgaaacaa taacatatgg
    18001 ctcaatattt aacataatga attatgcaca aaaaccaata tcatatatta caacaggaca
    18061 aaatgttcct gatgatatta taaaacctaa tgaagataag attataaggt tactattagg
    18121 agttgaaagt atatgataga ccaagctcaa attttgagaa aaatggcaat ggaaaaaaga
    18181 ggacttgatg agtttatagt agagaatgag aatgtaccta agataattac aatagcttct
    18241 ggtaaaggcg gtgttggaaa aagtaatctc gctacaaatt tgtctatatg cttgaccaaa
    18301 cttaataaaa aagttcttat attagatgca gacataggaa tgtctaatat agatataata
    18361 atgggggtaa atgtaaaagg gactataatt gatgtaatta atggtgaaaa aaatatagaa
    18421 gatataattt cacagactaa gtatggtgta aatataatat ctggaggttc tgcccttaat
    18481 catatagaag actttacaga agctcaaaga aataagttta tacatagcat agaacagatt
    18541 cacgatgtgg attttataat aattgataca ggagctggta tgagcaaaag cctattgtca
    18601 tttatatact gtagtactga atttttctta ataacaactc cagaaccgac atcattgact
    18661 gacgcatata gtttattaaa agctataagt aattttggca taaagagaag tgcaaatata
    18721 ataatcaata gggtaattga tttagaagaa gcaaaatcta catacaaaag aataaaaatg
    18781 gttgtggaca aatttctaaa ccttaatcta gaattgtatg gatatataat agatgacaaa
    18841 aaagtaagtt tatgtgtaaa aaaacagata ccattcattt tagaatatcc tacaagtatt
    18901 gcatcaaaat gtattatagc catagcaaag agaatggtaa gtcagtcaag agaagatatt
    18961 gttgataata aaagttttct aagaaagatg tttagtatat ttaaaagcta aggaggcgta
    19021 gttaatgaat agagaagaat taataaaaga gaatatgcct cttgtaaaga gtatagcaag
    19081 aaaatttttt ataccaggga aaggttttga atatcaagac ttagtaaata gtggagtcat
    19141 tggattgatt gatgcaataa ataaatttga tgctgaaaaa ggggcaaagt tttctagtta
    19201 ctcttatata aaaataaaat ctgctatatt agatgaaatt agaaatcaaa gtccaatttc
    19261 taaacataat ctaactaaag taaataagta taatagagtg gtagaaaagt tgcaatctaa
    19321 attattaaga gagccaactt catatgaaat tgcaaaagaa ttaaaggttt cagaaaaaga
    19381 acttcatgat atcgaaagta atatagatat gttaaatatt gtatcattaa actatgtaat
    19441 ctttgaagat acaaatgaaa ctgtacaaga tgtaataagt gatagagaag aggaagctcc
    19501 agaaaatata atagaagaag aagaaaagtt agagattcta tccaaagcaa ttagtaattt
    19561 aaatgaaaga gaaaaattaa tactttcact gtactattat gaagatttaa atctaaagga
    19621 gatagggaaa gtgctaggtg tttcagaatc aagagtttca caactccaca gaaaatcaat
    19681 aagaaactta agaaacaaga taaaagaact taaatattct atatagatgg tgattgataa
    19741 gatgatacaa attatattaa tagtagtgtc tattttaata atagtaacta tagttataaa
    19801 tttacataag gaatcaaaaa ataaagataa cagatacttt gataaaatat ttgagcaaga
    19861 atatgaagca aatgatagat taataattgc agaaaaaaga agattctttt tagacaaagt
    19921 acttgcagaa aaaagaagta atagtcatat aaaagtttat gaaaattata acctaataaa
    19981 ggctaatgaa gaaagaaagg aaaacatatt tatagaatca aaaaaggaag taagacaaga
    20041 aaatgagtta tctcttaaag tgaaacatct tataagttca ggttatgatg atgtggaaat
    20101 atgtaagatg ctagatattg gaaaggggga actgttatta ataaggagtt gttacaaaat
    20161 ataaggatat tattatgtga taaaaaagta ttcttaggaa taggtattgg tatattaata
    20221 tcaactttat gtataatgct ttttagtaat aatgatatga gtaaggcaga gatagaaaaa
    20281 aaggccagag cgttaggcat gaaatataca agtgagatga aagttttaga atagatgata
    20341 ttgtttagac atttacaaaa aaggaggtca taatgtgata agaggattat atacagcagt
    20401 ttctgctatg ataactaatc aacaaaagca aaatgttgtt gtcaataacc tagctaatat
    20461 ggatacaaat ggatataaaa gtaaacaact tttgactaaa agttttgatg aattaaagtt
    20521 ggagggttac aataactacg caaatggaac taaacaaaag cagattatag gcgatataag
    20581 tccaggtatt tctatggatg aaactatcac aaattttaat caaggaccta taaaaactac
    20641 tgataataaa atggatgtag ctattcaagg taagggattt tttctagtta gtgatgcagc
    20701 tggaaatcaa ttttacacta gaaatgggaa cttcagaaca gacaatcaag gtgaccttat
    20761 aacaagtgaa gggtattatg tacttgggac gaatactgct actggtgctg taggtcatat
    20821 aaatgtagga aatcaaaaat ttgaagtggc aaaagataat acaatatcac tagacggaaa
    20881 tgctatgtat aaatttaata ttgtagattt taataactac aataacttaa aaaaagaagg
    20941 agataactta tattctggag gaggagctat gaaagtaaat aatgcactta caatacaaag
    21001 tgcgatagag tcctctaatg tagatatggt atctgaaatt aataatatga tgacaatatc
    21061 aagagaatat gaggcaaatc aaaagataat tcaggctatg gattctaaat tagcaaaaat
    21121 agcaagtgag attggttcag taagataggt aataggaggt gaatttttat gtacacaatg
    21181 ttttatacta gtaaaacagc tttatcagca aatcaaagta aactagatat aatatctaac
    21241 aatatagcaa attctgattc aacaggatat aaaaaaataa atatggaata ttctgattta
    21301 gtggatgaag tactaaatag accatcatac cctacaaatg gtaaagatat atcgactgga
    21361 acaggagcta aggcaacagc tcccatgaga aattatgaac aaggtgcatt ggctcctaca
    21421 gatagtaaga gtaatttggc gatagatgga gaaggattct ttagagtgat aagaaaagat
    21481 ggtacatatg catatacaag aaatggtgga tttaatgtag atgctttagg taagattgta
    21541 gatgataatg gaaatatatt agatgtccaa tttgatgcag gatataacta taataataca
    21601 aatatagata gtacaaacct gacagtttca agaaatggag agctattcgt agataataaa
    21661 aaaataggaa aaattaactt atatcaacct ataggaactc aaaattttat atctgaagga
    21721 gatagtttat ttgtcgcaac tgatgcagca cagattaaac aagttgagaa agtcgatata
    21781 gttcaaggct atagagagag gtcaaatgtt tctttacaag aagaatttgt tgacttaata
    21841 gccactcaaa gagcatttca aatgaactca aagggaataa agacagcaga tgaaatgtgg
    21901 caaatagcga ataatttaag agctaaataa ttatgtaagg tgaggaatat tcatgaatga
    21961 tatgaaagag tacgattttg gcaaacctca gagttattca agagaaaatt taacttgttt
    22021 aaatttttta ttagctgaat tttgtaaaaa atataaaaac tatataattt atgaacttaa
    22081 gtgtaattct agtatggttg ttgatagtat agaccaagta aattatcaga cttttttaga
    22141 tagagtaact tcttcatgcg tagtagtaca aaatgcaatg gaacctgtta tgaaaaactt
    22201 ttcttttaga atagatagaa gtgtagctga tatgtggatt gatataactt ctggaggaac
    22261 tggagttgta aaggataatg atagagagct tacagaactt gataaaaaag tgcttttaca
    22321 ccttatggat gatttagtaa gaaatatgca cttatttgag ggttttgaaa atatacaagt
    22381 attggataca catacacata ttaatttgcc acaattatgt tctccaactg caccagtatg
    22441 cgttgtagat ttaaaagtgc taaatgataa tgagtatatt ggaagtgttt ctctttgttt
    22501 tccatacaat ggtttagagg ctctgttaga aagtatatct gttatagaat tttttgataa
    22561 tgatgtagga gatgattcag aggaatttac ccaaaaaatc tataatagtg tatctaatat
    22621 agaattatca gttattgcag agataggaaa agtatcaatt aatgttgagg atttacttaa
    22681 actagaagtt ggagatgtaa tagtaacaaa taaaaaaata aatgattata tagatatatt
    22741 tgtagaaaat tcaaaatcat acacagcaac accaggattt atatcaagta aaaaaggtgt
    22801 aaaaataaat gatgctgttg gaaaagaggt gtagttatgg acaatagtaa tcttatttca
    22861 gatgaagaag caaagatgtt attaaatgat aaagttgaaa gtgaagttga tggagatgat
    22921 gagttagcct tagatgtctc tgaagttaat cgaaataata tacaaaggat acttgacctt
    22981 aagttagaat tatctgtagt tataggaaga actaagatga ctctaagaga tattttaaat
    23041 ttacataaag gttctcttat tgaactggat actttagcag accaagatgt ggaaatacta
    23101 atagataata aggttttggc ttatggaaaa gtagtggtgg tagatttaaa ttttggtgta
    23161 aagataacaa gtatagtaag tgaagaagac atggtaaaat ctcttgttta gagataacat
    23221 caattaatac ttaaggcatg aggtggtact tattttaaac ttaaatgata taaaaaaaga
    23281 tatactaaaa ctcaataaaa aagactcatg ttctctatat aatgaatcta ttaataatat
    23341 aaataataat tgtatatcaa ctggattaaa atttttaaaa caagcaagag atttaaaccc
    23401 agatgatgtt gatatattaa atctcacagg tcttgtaaac ttactaaaat gtaattttga
    23461 tgatgctgta gaaagtttct ataagagttc actttgtgaa aaaactgctc tgtgtgaaaa
    23521 gtatataaac atgcttacat ctgaggagtt tttaattttc tttgaaaagt acaatcaact
    23581 tataaggtta ataaataaag gaatgtataa agaagctata caagggttta aactcataac
    23641 tgaccaatac tgtgatttaa tagaacctta tgagttgctt gccttactgt atgacaagga
    23701 agaagaatat ataaaatttg atgaatgttt agaagtattg aagattattg ataaggaaaa
    23761 ttatttatta aatcaagaaa ataacacatt agatatgtat aatagtgcta atatacaaac
    23821 taaaagtaat ataaaagtag atgattataa gaatgagttc ttacataaaa gtagtagtaa
    23881 ccataaaatt aatgattata atgatgtaac taaagaaaag agttcaagca aaaaccagaa
    23941 aagttcagat tctaataaga aaaaaagtat taaaaaaaat aaatacctat atatagccat
    24001 aatattagga atattattag taggacaaag tatatatagt tcttataaga tgaataattt
    24061 aactgataaa atatctaaaa ataatgaaac caaaaatgaa gagtcaatag attctaactt
    24121 gactaagaaa gaaaataata tagaagaaaa tgaagatatg agtaaggctt ctaatataaa
    24181 taagagtaat aaaaacatta ataaagagaa tgatgtaaaa tctaaaaaaa atactgtaaa
    24241 aaatgaagat gataaagatt ctgacttatt tacagaggat gaattgatgt ctaaggcgaa
    24301 tactttaaaa tcagaaaaga aaactaaacc ttctatacaa ctatataaga aagttgcaga
    24361 tataggtgaa aataaaggga atacatctga agcaacttat caagttgcta ttctaagtga
    24421 aaagttaaaa gactataaaa ctgctgaaga atattataaa atgtatgtag aaaattactc
    24481 agaaaaagat gcttattttg atgaatctta ttataattta ggtatgatgt actataacaa
    24541 tggtgattta aaaaattcta aattgacatt aaagaagttg gttaataagg taccaaatag
    24601 tatgtataat aattcaaaag taaaagaaat tttaaaagaa gaatagttaa attagataag
    24661 aaaaataaat tattttattt taaaataata tgattataat atattgacat accattttaa
    24721 aaaatttaaa atattaatta taaaatgagg agggataaaa aatggaattt gaaaaaggga
    24781 gtatttcaat tcatacagaa aatatttttc ctataattaa aaaatggtta tactctgata
    24841 aagacatatt tataagagag cttataagta atggatgtga tgcagtaagt aaacataaaa
    24901 gacttgtatc actaggagaa atatcagaaa ataagtcatc agattataaa attacagtat
    24961 ctgtaaataa aggagaaggt acacttaaat ttattgacaa tggaattggt atgactgaag
    25021 aagagataaa aaaatacata aatcaagttg ctttttcagg tgctgaagat ttctttaata
    25081 aatataagga taagatggaa gagtcaaatg acataatagg tcattttgga ttaggatttt
    25141 attctgcatt tatggtgtct aaaaaggtac aaatagatac actttcatat acagaaggag
    25201 cgactcctgt aagatggata tctgaaggtg gtacagaata cgaaatatct gaatcagatg
    25261 ctagaaatga tagaggaact actataacac tatttattga tgatgatagt aaagaatttt
    25321 tagatgaatt tactgtaaga ggaataataa ataaatactg ttctttctta ccagtagaaa
    25381 tatatttaga agatgttgaa agattagaaa gagaagctaa agaagcagaa gaaaaagcta
    25441 aaaaacaaaa ggaagatgga aaagaagaga ttgtagatgc aaaagtaatt gagcctttaa
    25501 acgacactaa tcctctatgg ttaaaatctc caaaagactg tactgatgag gagtataaag
    25561 agttttatag aaaggtattc aatgtatttg atgaaccttt attttggata catttaaatg
    25621 tagattatcc tttcaattta aagggtatac tttacttccc taaattaaaa aatgagtttg
    25681 aactgactga aggaaaagta aaattataca acaatcaagt gtttgtggct gacaatataa
    25741 aagaagttat accagaattt ttacttttat taaaaggagt tatagactgc ccagacttac
    25801 cattaaatgt atcaagaagt ttcttacaaa atgacaggga tgtaagcaaa atttctaagc
    25861 atataattaa aaaagtagct gataagttaa aatctctttg taaaaatgaa agagaagaat
    25921 acaataagtt ttgggatgat atacaaatat ttattaaata tggttgctta aaagatgaaa
    25981 gtttctatga gaaggtaaaa gaatgtatat tattcaaaac tattgatgat gagtatataa
    26041 cattacaaga ttatttagaa aaatgtaagg acaaacatga aaataaggta ttttatgtaa
    26101 gtgataagga acagcaatct caatacatta aactatttaa agaatacgac ttaagtgctg
    26161 ttgttttaaa ctcatcaata gatactcatt ttatttcatt tatggaatat aaagagaatg
    26221 gagttaagtt taatagaata gatgcagacc tttctgatgt attaaaagat aaaaatgaaa
    26281 ataaagatag tgaagaaaac aaagaagaga tagctaagat agaaggtttg tttaaagaag
    26341 ctgtcggaga aagagtgaag aattattctg tagaaggatt gaaaaatgaa gacactccag
    26401 ctatggtatt agtatcagaa caatctatta gaatggcaga aatgcagtca agatttgctg
    26461 gaatggattt aggaatgaat tttgaggaag aaaaaactct tgtaataaat gagaacagtc
    26521 caataatcaa aaaattggtt tctcttaaag atgatgagga aaagaaagat aagattacgc
    26581 ttatttgtaa ccagatagca gatttagctt tactttctaa taaagaatta aagccagatg
    26641 agttagattc atttgtacaa agaagtaata agcttatgag tcttttgata gaattataag
    26701 gttgaatttg aatacattga aacaaattta taaaaagtat tcaatatcag tgatgagaaa
    26761 gaagtgtctc aaaatgaact tttcagttca tgaggcgctt tttttatgtg aaattaaatt
    26821 tattttaatc atttcaacaa tgaaaaatat atttatctct attttgagac agaccattct
    26881 ttattttttt atctttggtt aattttaaat atttaagtag gttctaataa aaaactctat
    26941 ttagttaaat atagttaact tgatatataa taagataata catataatta agtaaaagat
    27001 tgttttttag tttatgttta aatacatgat tacactttgt taaaaacata aaattattac
    27061 taaagtagaa ataggaggtt ttagcatgaa ttttaattat aatttgccag taaatatctt
    27121 atttgggaga ggcagaatta atgatgtggg aaaagaagta tctaaatatg gcaaaaaagt
    27181 attgatagta acaggtaaaa atagtacaaa gaaaactgga ttacttgata aaacaataga
    27241 tttactaaaa gactctaaaa tagaatatga agtttttgat agagtagaac aaaatcctct
    27301 tataactact atatattcag gagtagaaat tataaaatca actggatgtg attgtgtttt
    27361 agggcttggt ggtggaagta ttatggatgc tgccaagagt atagcttttt cttataaaaa
    27421 tccaggagat ttaaatgatt atatatttgg aattagacaa ggtgaagaag ctttacctat
    27481 aatcttagtt ccaacaacat caggtacagg tagtgaagga aattgttttg cagtacttac
    27541 aaattcagaa actaaagata aaaaatctat aaagaagaat agtatgtatg caaaagcatc
    27601 aataattgac ccagaactta tggttacaat gccaaaacat ataattgcat cagttggatt
    27661 tgatgcactt gcccataata tggaagcata tctttctaat ggaagaaacc ctcttgcaga
    27721 tgtacaagct atttatggga ttgaacttat atcagaaaac ctaataaagg tatataatga
    27781 tgttaatgat ttagaagcat gggaaaagat aacacttgca agtactattg gaggaatgac
    27841 gattggtaca gcgggtacag ccttgccaca tggaatggag catcctgtaa gtggtcttag
    27901 agatgtaatg catggaaaag gacttgcagc tttgactcct actattatag aaagttcatg
    27961 ggatagtgat attgaaagat ttggtaatat ttcaaaggta ttaggaggaa cttgtgcaga
    28021 agattgttca gatgcaatta gaaatttcct taaaaaagta gatttagata taaaactaag
    28081 tgatttaggt attgaaaaat cagatataga ttggttaact gataattgta tgaaaatatc
    28141 agttgcaaat attaagagac atccaaagta ttttaataag gaacaaataa aggaaatgta
    28201 ccataagtct ttataatttg actttacata tttagtttta tataaaaatt ataaattaat
    28261 aataaaaaat tatacgtaat taatagtaaa aaaattgtgt atagaagtta tatatacaat
    28321 aaacttgtct taagctttta ttttactttc taataaataa aggagtttaa aaaagggaag
    28381 attaattaag taatatttta taaaattctt cgatttaaaa actttaagta gaggtgaaat
    28441 aaatgtttaa tcccaaaaga ataatattcg agaaaggaac tttagacact gatgtgggta
    28501 aaaaaatata caataaattt agagataata aagatgtaga gataattaat atatcatcaa
    28561 ataggattaa agaacatatc ccaggtgaaa atctatatga acaatataga agtggaaagc
    28621 agactatggt agttgggatt agaaaaagtc tgaagtttca aacatgtaaa ccttctgcta
    28681 attatcaact accacttgtc agtggatgta tgggaagatg tgagtattgt tatttgaaca
    28741 cacaattagg tgataaacca tttataaggg tatttgtaaa tgtagatgaa atacttgaac
    28801 agacaaaaaa atatattgaa gatagaaagc cacaggtaac catatttgaa ggggcagcta
    28861 catcagaccc agttccagta gagccatata ctaattcttt gaagaaaaca atagagtttt
    28921 ttggaagaga ggaaaaagga agatttagat ttgttactaa atacagtgat gtggatagct
    28981 tattggatgc tgaacataat aatcatacag aaatacgatt tagtatcaat actcctagaa
    29041 ttataaatga acatgagcat tatacagcct ctgcaaataa aagaatagag tcagctgtaa
    29101 aacttgctag ggctggttat cagattggtt tcataatagc acctgtgttt ttatatgaag
    29161 gttggaaaga agagtataaa aatctgatta taagtataaa agaagcttta ccaaaggatt
    29221 ttgataaaaa aattatattt gaagttatat ctcatagata tacaactaaa gcaaaaaata
    29281 gaatactaga gatttatcca gatacactat tgccaatgga aaatgaagat agaaagttta
    29341 aatacggtca atttggatat ggtaagtacg tgtatgataa agaacaatta gatgatatga
    29401 aagagttctt tcttaaggaa ataaatggag tatttaaaaa tagtttaata aaatatataa
    29461 tatagcaaat cttacctaaa gatttgctat attttatttt tatatatttt atgtttataa
    29521 taaagcaatg agatttagca ttatcatata agaaaattaa aaagggcact atctatcaag
    29581 aacatttttt ctagttttga tactataaag gataagagta acaaaaatat caggaggagt
    29641 tatagtatgc atgcatggga gtctattcaa attacattgg atttaataga agcaaattta
    29701 tcagaagaaa tatcaattaa tgaattagca aataaagcaa atttatcacc attttactat
    29761 caaagactat ttaaaagatt ggtaaataag actgtaatgg agtatataaa gcttcgtcgt
    29821 cttgcaagag cttctgaata cttagcagaa cataagaatc gtatacttga tgtagcttta
    29881 aattttggat ttgctagtca tgagacattt acacgttcct ttaaaaaagc atatggatta
    29941 actccagaaa aatatagagc aaatccagta aaactcaatc attttattaa gccagaatta
    30001 attttaaatt atgctatggt tgatgaaggt gttccactga ttgcagatga tatagtgatt
    30061 gaagtcaatc gtaagacatt gagtaatcca aggacatttg ttggaataga aatagaagta
    30121 cctatttgcc agttggtggg cggtgaaaca actggaattg caatagtaga ggaattatgg
    30181 atgaaacttg gaagtcaaag acataatata ccaaatcaaa ttccaggtgg aaatgaattt
    30241 gctgctcttt atatgggaaa tgcaaaagaa ggaaattgta tgtatatggc aggtgtagag
    30301 gtagagtcag gtacatcagt agaaggatat agtacttttg agttgccagc taaagaatat
    30361 ttagtatgtg gatttgaggc agaaaacttt aatgagttag ttaattcagc tgtttttaaa
    30421 gcagataaat ttatggaaag atggatgaag aaacataatt taacaactac tgattttgca
    30481 attgagatgt attaccccac tacacctgaa gcagcatatc tggaacattg gattgttccg
    30541 gttcctattg aacaatagaa aggatgatga atatgaattg gcaagatgcg ttttttcaag
    30601 atactaaacc tacatttgag caaattaatg aatttattaa taatccatta tgggacaagt
    30661 taaataaaac cttggtttct acttataatg tagaacctaa gcttgaatac agtaaatgtt
    30721 caatgcaaag aggttggaat gtaaaatata aaaaaagagg taaatcacta tgtactttat
    30781 atccacaaga aggttatttt aaagctttgg ttattgtaag tgaaaacaat agagtagagg
    30841 ttgatttatt tattaatact tgttgtgatt atatcaaaaa aatatataat gaggtaaact
    30901 tttttaatgg aagtaaatgg ctaatgattc aagtagatag tttattggta ttaaacgata
    30961 tgcttgagct aattaaattt agagcttaac atatagaaca ggatgaatat ataagtcatc
    31021 ctgtttttat attcaatcag ataaattaat atatagtcta taatatgaat aatttagtaa
    31081 atttaaattt actttatctt tctacattct ttgcaactaa gtccacatgt gtttttttct
    31141 tgcatattag tagcacccca attagcaaat tgttgtaaaa taggcacaat acttttacct
    31201 ttacttgtta atgtatattc aacttttgga ggaatttcat tatattgttt acgatgaaca
    31261 ataccagtag actctaaatc ttttaatgat tgagctagca tagtatttgt aatgccatcc
    31321 aattttcttt ttatgctact atatctaatt ggattttcaa ctgctaacat acatataata
    31381 ggtaatttcc acttaccacc gacaatgtct aatgcataac ggattggaca ggttaattct
    31441 tcaacgtttt tagttatatt tttttcagta tttatactca ttttaattac tccttagtta
    31501 tagtagtatc ttttttatac tttatcagaa aaaactgcat acttgttttt tctgtatttt
    31561 atattaaaat tatatcaaag gataatcctt aatacaagta tttaaaaggg gtgtaagaat
    31621 tatgaaagca ttagaatttt tgaacaaggc aggagtttat tatttagcaa cagttgatga
    31681 gaacaatcaa gcacatgtgc gtcctttagg atttgttatg gaatacaatg gtaaacttac
    31741 attttgcaca tctaatcaaa aggatatgtt taagcaactt atttttaatc caaaggttga
    31801 actttgttgt atagaccaaa accttaatac tctacgtatt ttaggtgaag cagtatttgt
    31861 tacatcaaaa gaaacacaag ctaaggcact tgaaataatg ccaagtttag gtcagatgta
    31921 ctctgtggga gatggtaagt ttgaaatatt ctctattgat aaggcaaaag ttagttgttg
    31981 ttcaatgagt ggtaaaaaga ttgatgtaga gctttaatta taattgaatc gacatccata
    32041 gtgaaaatta agaaaacgtt attactgaaa tattgataga tttcttttaa aaaatatttt
    32101 aactataatt gatttttatt taatctgaaa aaaatattat gactataaaa aattgctact
    32161 tattatttgg atatttttgt atataaataa taagtagtat gactttatac attaataatg
    32221 atagaatata aattaataga taagatatga ttttaaaagg agtgatataa gatgagtaaa
    32281 tatagttctt tatgggaata tgttaagaaa aacaatagtc aatcgtttaa attaactttt
    32341 gaagaaataa aagatattgc tgggatagag atagaccatt cgtttttgaa atataaaaag
    32401 gaattaaatg aatatggata tcaagtagga aaaatctctt taaaagagaa gactgttatt
    32461 ttcgataaaa ttggttaaga cataaataat tacatataat aaaataattt gtattgatga
    32521 ttactacaaa taaaaaaagg cgtagcacta ttttttagtg tcacagcccc ttttttgttt
    32581 ataattatgt acatttcaca ttatcacgat ataagcattt ttcttatgag aaaattaatt
    32641 agaattattt ttattcatat ttaaattatt taaattgttt tcccagtttt ttatctcctt
    32701 tttaacttct tcacttaaag tatcacttga agatattttc tttaatttat cattcaaaga
    32761 tttcttttgt tcctctgaaa gatctgtttt tattgtagga tcaataatat ctattacatc
    32821 ttttacatca aaatcaacaa atttgcacag ttcttctaga tgatcaatta gaaaatctag
    32881 accttcatat gaccattctt tataatcttt tccgtttttt aacagaaact cattcacttg
    32941 gtaattctga tttgctaatt caaagaatga acctgaagga tccattatat catttataga
    33001 gttttcatag ttaacaatat taatttgtct tattaatttc tctttttctg ttaagttgct
    33061 ataattcttg ttaacaaaag ctactaatcc aaattcaaag tttaaaattg aatctttacg
    33121 tttttttact gaatcttctc caaattccct aagaccttct aaatttttat caatggcttt
    33181 atctctaatc tctattaatt gttctatact taaaccttct aagtttttag gtactatttc
    33241 ttcattattc ttagtaactt ctttcaattt attgttagta tttttattat tgaaaataaa
    33301 tatagcagtt aataaactta aaattattac taaaataatt aaacttacat tttttttact
    33361 taacatatta aacctccttt tttaaataag tattgtactt gtgaaaaata aatttgttta
    33421 tgaagataac atttactact gtttgttcta aagattataa tagaatattt ttttgatata
    33481 atctatattt tt
//
LOCUS       DAEMHZ010000035        32065 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000035 DAEMHZ010000000
VERSION     DAEMHZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.035"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            108..224
                     /gene="rrf"
                     /locus_tag="KPK72_001495"
     rRNA            108..224
                     /gene="rrf"
                     /locus_tag="KPK72_001495"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            344..535
                     /locus_tag="KPK72_001496"
     CDS             344..535
                     /locus_tag="KPK72_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG2488725.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            690..2099
                     /locus_tag="KPK72_001497"
     CDS             690..2099
                     /locus_tag="KPK72_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2488726.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2230..2919
                     /locus_tag="KPK72_001498"
     CDS             2230..2919
                     /locus_tag="KPK72_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG2488727.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2922..3857
                     /locus_tag="KPK72_001499"
     CDS             2922..3857
                     /locus_tag="KPK72_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG2488728.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3854..4747
                     /locus_tag="KPK72_001500"
     CDS             3854..4747
                     /locus_tag="KPK72_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG2488729.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4825..5571
                     /locus_tag="KPK72_001501"
     CDS             4825..5571
                     /locus_tag="KPK72_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG2488730.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5572..6405
                     /gene="rsmI"
                     /locus_tag="KPK72_001502"
     CDS             5572..6405
                     /gene="rsmI"
                     /locus_tag="KPK72_001502"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG2488731.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDILKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6428..7132
                     /locus_tag="KPK72_001503"
     CDS             6428..7132
                     /locus_tag="KPK72_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2488732.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLNVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7320..7748)
                     /locus_tag="KPK72_001504"
     CDS             complement(7320..7748)
                     /locus_tag="KPK72_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2488733.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7785..9215)
                     /locus_tag="KPK72_001505"
     CDS             complement(7785..9215)
                     /locus_tag="KPK72_001505"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG2488734.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9347..9931
                     /locus_tag="KPK72_001506"
     CDS             9347..9931
                     /locus_tag="KPK72_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG2488735.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9947..10468
                     /locus_tag="KPK72_001507"
     CDS             9947..10468
                     /locus_tag="KPK72_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBG2488736.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10534..10780
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10864..12801
                     /gene="metG"
                     /locus_tag="KPK72_001508"
     CDS             10864..12801
                     /gene="metG"
                     /locus_tag="KPK72_001508"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG2488737.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKGELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGEPYVLPFTQGAKDGCEVR"
     gene            12831..13601
                     /locus_tag="KPK72_001509"
     CDS             12831..13601
                     /locus_tag="KPK72_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG2488738.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13938..14369
                     /gene="phnG"
                     /locus_tag="KPK72_001510"
     CDS             13938..14369
                     /gene="phnG"
                     /locus_tag="KPK72_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG2488739.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14384..14959
                     /gene="phnH"
                     /locus_tag="KPK72_001511"
     CDS             14384..14959
                     /gene="phnH"
                     /locus_tag="KPK72_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG2488740.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14962..16047
                     /locus_tag="KPK72_001512"
     CDS             14962..16047
                     /locus_tag="KPK72_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG2488741.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFISELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16067..16936
                     /locus_tag="KPK72_001513"
     CDS             16067..16936
                     /locus_tag="KPK72_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG2488742.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16929..17795
                     /locus_tag="KPK72_001514"
     CDS             16929..17795
                     /locus_tag="KPK72_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2488743.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17836..18549
                     /locus_tag="KPK72_001515"
     CDS             17836..18549
                     /locus_tag="KPK72_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2488744.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18550..19719
                     /locus_tag="KPK72_001516"
     CDS             18550..19719
                     /locus_tag="KPK72_001516"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG2488745.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19723..20559
                     /locus_tag="KPK72_001517"
     CDS             19723..20559
                     /locus_tag="KPK72_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2488746.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20560..21555
                     /locus_tag="KPK72_001518"
     CDS             20560..21555
                     /locus_tag="KPK72_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2488747.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21573..23261
                     /locus_tag="KPK72_001519"
     CDS             21573..23261
                     /locus_tag="KPK72_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2488748.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23273..24340
                     /locus_tag="KPK72_001520"
     CDS             23273..24340
                     /locus_tag="KPK72_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2488749.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24777..24893
                     /locus_tag="KPK72_001521"
                     /pseudo
     CDS             24777..24893
                     /locus_tag="KPK72_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
     gene            complement(24916..26274)
                     /locus_tag="KPK72_001522"
     CDS             complement(24916..26274)
                     /locus_tag="KPK72_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2488750.1"
                     /translation="MLRVALYIRVSTEEQALNGDSIRTQIEALEQYSKENDFNIVGKY
                     IDEGCSATNLKRPNLQRLLRDVEKDKVDLVLMTKIDRLSRGVKNYYKIMETFEKHKCD
                     WKTILENYDSSTAAGRLHINIMLSVAENEAAQTSERIKFVFQDKLRRKEVISGTIPIG
                     YKIENKHLVIDKEKKYIVKAIFDEYEKSGSVRTLIETINNLHGELYSYNKIKNILRNE
                     LYIGIYNKRGFYVEDYCEPIISKKQFKQIQRILEKNKKTTPNKNIHYHIFSGLLKCKE
                     CGYTLKGNSSNVGEKLYLSYRCSTFYLNKNCVHNVTHNEKHIENYLLTNLKPQLHKHM
                     VKLEAQNEKIRQNKKSNKKDEKKKIMKKLDKIKDLYLEDLIDKETYRKDYEKLQSQLD
                     NITEEQESQIIDTSHIKKFLDIDINEMYSDLSRVERRRFWLSIIDYIEIDNDKNITIN
                     FI"
     gene            complement(26339..26812)
                     /locus_tag="KPK72_001523"
     CDS             complement(26339..26812)
                     /locus_tag="KPK72_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2488751.1"
                     /translation="MNSYEKLLSEADDNNIIVREVSLISNSHGLYKNNRIALNKNTLN
                     NMSEKACVLAEELGHHYTSYGNILDLNKVENSKQEYKARLMAYNKLIGLKGIIDSFNA
                     GCKTITEMAEYLDVTEKFLNEALECYKSKYGFSATLDNYVIFFEPRFSIMNANFL"
     gene            complement(26830..27285)
                     /locus_tag="KPK72_001524"
     CDS             complement(26830..27285)
                     /locus_tag="KPK72_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488752.1"
                     /translation="MNTENDLKMLEITNRIKNKRLELNMSYQDLANKTGLSKSTLQRY
                     ETGAIKNIPLDKLEILAHALDESPAFIMGWNEDCKQIKIKENKLLSSFNALNDIGQNE
                     AIKRVDELTQIGKYVNKNHIDTIAAHNEHLHEEGEIEKIYQDLDDMDNW"
     gene            27439..27660
                     /locus_tag="KPK72_001525"
     CDS             27439..27660
                     /locus_tag="KPK72_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488753.1"
                     /translation="MNISKLKGKMVEKKYTQKKLAEELGMTVQALNAKLNNRSQFKIE
                     EAVKISNILQIEEPSEIFFDNYIPKMQRN"
     gene            27708..28502
                     /locus_tag="KPK72_001526"
     CDS             27708..28502
                     /locus_tag="KPK72_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG2488754.1"
                     /translation="MSNNLQVIERNNKRVLTTQQLADVYETDSKNISNNFNNNKDRFI
                     EGKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDN
                     LEETYFKVKQQKPTCIEDVLIESLKEMKDLRLQVNQANNIALEAKTEVKTIKEVVSLN
                     ATDWRKDTQQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEE
                     GVSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            28560..28757
                     /locus_tag="KPK72_001527"
     CDS             28560..28757
                     /locus_tag="KPK72_001527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488755.1"
                     /translation="MRNKEKEAIINSNSAKKVIELKHEQTTLRIPEDLYKALIDLSRE
                     IGMPIASIVIIACWLYISKIN"
     gene            28768..28992
                     /locus_tag="KPK72_001528"
     CDS             28768..28992
                     /locus_tag="KPK72_001528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488756.1"
                     /translation="MQNECTALFIRELTDKLFSLAVFSSNSFRTLGILRVERTLSSLL
                     YETDLSSLTFEFLIMSLIAYRISEDLRIPV"
     gene            29176..29346
                     /locus_tag="KPK72_001529"
     CDS             29176..29346
                     /locus_tag="KPK72_001529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488757.1"
                     /translation="MEKIRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            29349..30137
                     /locus_tag="KPK72_001530"
     CDS             29349..30137
                     /locus_tag="KPK72_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBG2488758.1"
                     /translation="MNNLQIFKNNTFGEIRVIEIKEEPWFVGKDVAEKLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMRLINESGLYSLILSSKLPNAKAFKRWVTNEILPSI
                     RKTGGYIHTTENMSDDEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADAVA
                     SSDDSILVGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLKIM
                     ETKKRAIYNPDGSTKITKTPKVTGKGQIYFVNKLKSVNQISILS"
     gene            30198..30386
                     /locus_tag="KPK72_001531"
     CDS             30198..30386
                     /locus_tag="KPK72_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488759.1"
                     /translation="MGKKSEYTVTTVGEPNLDALGETLAKILTEKFGVLITHTYIENY
                     YVDKERGGDVDGAIESEL"
     gene            30361..30588
                     /locus_tag="KPK72_001532"
     CDS             30361..30588
                     /locus_tag="KPK72_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488760.1"
                     /translation="MELLKVNFNLEKLKIGNVVSVSSKRFGFNINCIVVVATEEELNL
                     AYYSKDKGCIEYQALIPEDIRYGDYILERLG"
     gene            30686..31003
                     /locus_tag="KPK72_001533"
     CDS             30686..31003
                     /locus_tag="KPK72_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488761.1"
                     /translation="MESLIIVRNAVEQQLNRANLEINKNEQLYTKLRKKEERNILEEI
                     ELSNALREKSANERLKIFAESLLELLDTQIKIKENEESEDYKRLCMYLDEFGRDRPID
                     VQI"
     gene            31089..31598
                     /locus_tag="KPK72_001534"
     CDS             31089..31598
                     /locus_tag="KPK72_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG2488762.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIISVIINIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVIKVDKNSIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            31608..>32065
                     /locus_tag="KPK72_001535"
     CDS             31608..>32065
                     /locus_tag="KPK72_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG2488763.1"
                     /translation="METNNIYMKLVNIQSALKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYS"
BASE COUNT        12745 a         3628 c         5605 g        10087 t
ORIGIN      
        1 attaagatat atgtgtagtt tttagagtgc taactctaaa gaaactagta ttactaggtt
       61 ctataaactt attaaaaact taatagaaat attaagcata aagattatgt ggttacaata
      121 gcagagagga tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg
      181 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtaatttt ttttattttt
      241 tgtcaaaaaa aacaaaaaat acgtatttct tttttcattt cgggaaaact aaacacagga
      301 attatggact ttataaaaat ttaattcttg aggagtgata taagtgaaaa aagttgcttt
      361 aattttagct ataataggtg cgcttaactg gggtgcaatt ggtattctag gtacagactt
      421 aataggtagt atctttggtg gaacatatga aatggtaagt agaataatat acttcattgt
      481 aggtttagct ggattatact taataccaag tttaatgtct gatgatagag aataatttat
      541 gcttttatgt taaatacctc attctaaatg tttagaatga ggtatttaat tttataaaaa
      601 tattaatttg tttctttttt gatggtaaaa tagataagaa ttgaagtttt aaaaagaagt
      661 attatgtaaa aaaggagtac tattaatata tgtttattat taatgaatta aataagattg
      721 taaatgaaga agtaatatct tttcatatgc ctggtcataa aaagggacag gtttataaaa
      781 agttaggata taaagatata ctaaaaaatt tgtataaaat ggatacaact gagatattag
      841 gaacagataa tttacacttt ccggaaggaa ttataaaaaa ttcacaaaaa aaagcatcaa
      901 gagtatttaa aagtgattat acttgctatt tagtaaatgg tagtagctgt ggaatacaat
      961 ctgcaataat gtcagtatgt agtcctaaaa gtaaaataat aacaaataga gactgtcatc
     1021 agtctgttat aaatggatgt attttaggag atatagatat agagtatgtg ccttgtgaga
     1081 taagtgaaga gacaaatatc ttaaaaggga tatctataag taatgtgatt gatataatag
     1141 ataaaaactt ggatgcaaaa gcagtactgc ttacttatcc tacatattat ggaatgacat
     1201 atgatttaga atacatatgt aattatgcac atagcaaaaa gatggtagtg atagtagatg
     1261 aagcacatgg tgcgcattta gggcttagtg agaggcttcc taaaacagct cttgagcaag
     1321 gtgcagatat agttgtacag agtacacata aaactcttcc agcatttaca caatcgtcaa
     1381 tgatacatat acagggagac cgagttgata tagataaaat attgaacatg ttaagaatta
     1441 cagaatcttc aagcccttca tatttattat tatcttctct ggaattagca gtagatatat
     1501 atgaaaataa aggaaaagaa ttaatggaag agcttttaaa taatattata actttcaaaa
     1561 ataaagttga ttcaaatatt aatatatacg acacaaatga taaaacaaaa atatttatat
     1621 catctaaaaa catgggttta acaggttatg aactagaaaa tattttgaga aataaatata
     1681 acatacaagt ggagttatct aattattatg gagttctttt aatatgtaca ataggaaata
     1741 ctgctgagga tttctctagt ttagaaacag cattaaacaa tgttgtaaag gaagacttta
     1801 aatttgaaaa gttagaaaat aaaaaatacc ctgttgttat tccaaagaaa atattaacac
     1861 ctagagaagc attttataaa actaaaaaaa gtgttaaaat atatgacagt ataggtaaag
     1921 tttgtggtga atgtatagtt ccatacccac ctggaataag tgtaatatct cctggtgaag
     1981 taataagtaa agaaataata gactatctaa tgttttgtca ttctaaaggc atgattgtga
     2041 gtggattgaa agatgtagat ttaaatttta tacaagtaat agatttagaa gacatttaaa
     2101 aattatgata taaaagtaga tagaagtaga tagtttaaaa ttgtgaagag aaataaatag
     2161 actgcttata tagagatatc taataaaagt actaaaaaag taaaaagtgt ttgtatggag
     2221 gatatgaaaa tgaaaggtaa gttaataatt atagagagtg gttcagatgc aagtggaaag
     2281 gctactcaga cagaaatatt atatgaaaga ttaaaaaaag aaggctatat gattaaaaag
     2341 gtagaatatc caaattatga atctccatca tctgccttgg tgaaaatgta cctaaaagga
     2401 gactttggta aaaatgcatc agatgtagac ccatatatag catcaacttt ttttacagct
     2461 gataggtatg catcatttaa aaccgattgg gaggagtttt acaatgaagg tggaataatt
     2521 atcgcagata gatatacaac ctctaatatg gtacatcagg cctctaaaat ggatatttgt
     2581 gatagagaag agtatttaaa ttggttagct gattatgaat ttaatttatt taagatacca
     2641 caacctgatt gtgttatatt tttagatgtg cctattgaat ttagtaaaaa gcttatggag
     2701 aatagaaaaa ataaaattac aggggaagaa aaaaaggata ttcatgaaag tgacatagag
     2761 tatttagaga aatcttacaa caatgcttta tatatagcag ataaatatga ttggaaaaaa
     2821 ataaattgtg ttttagataa caaattaaga agtattgaaa gtatacataa tgaaatttat
     2881 aaaatagttc ttgattcaat aaaacctatg gagagataaa tatgtatttt gaaaatatta
     2941 tagggcaaga ttttgcaaag aaatatttaa caaattctat aaagaaaaat aaacttaata
     3001 atgcgtatat gtttgaagga atggatggaa taggaaaaaa gaaatttgca gatgaattat
     3061 caaaactatt acttgattat gaaaatctcg aaaatagtcc agactatgta ctcataaaac
     3121 cagatggaaa tagtataaaa atagctcaaa tcagaaattt acaatcagat atagtaataa
     3181 gacctcataa agactataaa atatatataa tcaataatgc tgaaaaaatg actgtggaag
     3241 ctcaaaatgc acttttaaag acgctagagg aacctccaaa ctatgctata ataatcttag
     3301 ttacaaacaa caaggaatct ttattagaaa ctataaaatc tagatgtgat ataataaagt
     3361 tctcacctat accaattgaa gacttaaaac gttatttgat aaatacaggt atagaagaag
     3421 aaagagctca attattggct attttttcga gaggaagtat tgagaatgct ctaaatctat
     3481 ctcagtcttc tgagttttca atgatgagag aagatataca acagtatatt cagataatgt
     3541 tagataaaaa catagtggag attttaaata taccaaataa tatggaaaaa tatagaggta
     3601 agattatagc tttattagat atgatgataa actattttcg agatatcatc ctattaaaag
     3661 aaaatgtaaa caagaacatg ttaataaatg tagataagct agttttcatt caaaatatga
     3721 atggaaaaat tagttattct caattgtcca agattattga tataatagaa gatgcaaaga
     3781 gtaaaataaa aagcaattgc aatttcaata taagcataca agttatgtct ttaaatatat
     3841 acgaggtgat taaatgataa agatagtagg tataaggttt aaaagtgctg ggaaaatata
     3901 ttattttgac cctgttgatt ttaatataga gcaagatatg gatgtagttg tagaaactgc
     3961 aagaggcttg gagtatggga aagtagtcgt tggtccaaaa gatatggatg aaagcgaatt
     4021 agtttctcct ttgaagccaa ttataagaat agctacagaa gaagataaaa aagtatatct
     4081 agaaaataag gaaaaggcaa aagaaacttt tgagttgtgc caacaaaaaa taaaagaaca
     4141 tgagttaaca atgtttttaa tagattgtga gtatacgttt gataggaata agttgatttt
     4201 ttactttaca gcagaaggaa gaatagattt tagagaattg gtaaaagact tagctgctat
     4261 atttaaaact agaatagaac ttagacaaat aggtgtcaga gatgaagcta aatctatagg
     4321 aggtctaggg ccttgtggta gaaaactttg ttgttcatct tggttaggtg attttcaacc
     4381 agtttcaata aaaatggcaa aagaccaaag tctatctcta aatccaacaa aaatatctgg
     4441 aatatgtggg agattattct gttgcttaaa atatgagcat gatgtatata gtgaagcaat
     4501 agaaaaaatg cctgttgtag gtgcattggt gcaatcagat gatggaaaag gaaaagttgt
     4561 agaagtgaat cctttattag aacaaattaa agtggaattt caagacaaga cagtaaagat
     4621 atacacaaga gaagatataa aaatacttag agagcctaaa aaatgtgatg gctgtggtgg
     4681 taaatgtaag gataatgatg gattagatga agccacacta agagagctaa agaaattaga
     4741 agattaaatt taaggtaggg cttttcctac cttttttaaa gcaattaatg cttaaagtta
     4801 ttaatatttg aggagaattt atatatgcaa ttaaaagatt cagaaagaat agatgattta
     4861 caattaaaag gccttaaaat tatacaagat actaatggtt tttgttttgg tatagatgca
     4921 gttttattag ccaattttac taaaataaaa aaagatgcca aagtagtaga tttgggtact
     4981 ggaactggaa ttatacctat tttgatagct ggaaaaagtg aagctaaaaa aattattggc
     5041 gtggaaatac aagaagatgt atatgaaatg gcaactcgtt ctataaaatt aaatggatta
     5101 gaagaaagag ttgagattat aaatgaagat ataaaataca tagacaaagt cttagacata
     5161 aatggttacc atgtagtcac atcaaatcct ccatatatgc atattgatgg aataaaaaat
     5221 ccaaatgata aaaaagcaat atcaagacat gaagtaaggt gcaatttgga agatgtaatt
     5281 agagctgcat ccagattggt tatgcctaga ggaaaattct tcatgataca tagaccaaca
     5341 agattggttg atataataac tttagggaga aaatataaat tagaaccaaa ggttattcaa
     5401 tttgtgcatc caagacctca aaaagcaccg aacttagttt tggttcaatt tgtaaaagat
     5461 ggaaggccag agcttaaaat attggaccca ttgtatgttt atggagaaga tgggaattat
     5521 acaaaagaat taaaagcaat atataataat aaagatatag gagagttgta aatgagcggg
     5581 aaattgtaca tatgtccaac accaatagga aacctagaag atataacata tagaacttta
     5641 agaatattaa aagaagtaga cttgattgca gcagaagata caagacatag tataaaactt
     5701 ttaaatcatt ttgaaatctc aaaaccacta actagttatc atgaacataa taaggattca
     5761 aagggagatt atttgataaa taaactaatt gatggtgaaa atatagctct gataagtgat
     5821 gcaggtatgc ctggtatatc tgacccagga gaagagataa taaaacaagc tatacaaaat
     5881 aacatagaga tagaagtatt gccaggtgca acagcctttg taacggcttt agttggctct
     5941 ggtatggaca cacatagatt tgtttttgag ggctttttag atagagataa aaaagttagg
     6001 aaagcacaat tggaagaatt aaaggaagaa agtagaacta taattttcta tgaatcacca
     6061 catagattga aggatacact aaaagatata cttaaaattc ttggtaatag gaatatttct
     6121 ataaatagag aacttacaaa aaaatatcaa gaaataataa gagaaaatat agaaactacc
     6181 ataaaaatat ttgaggaaaa agaagtaaga ggagagtttg tattaatagt agagggtttt
     6241 catggtgaga agtcaaagaa agaaaattat gatgggctta cagataggga atatgtttta
     6301 aaactaattg aaagtggtat gaacaaaaaa gatgcaataa aaatagtttg taaagataga
     6361 aagttaaaga aagatgtggt ttataaacaa gttttagatt tataaaacct gaatctgagg
     6421 tgattttatg gaaataggaa aaattataat agagagttta aagagggaag atacacataa
     6481 aatatttata gaattagata ataagcatga attagaaaaa ataattccta aaataagctg
     6541 tatgaagtct gtaggtgaat gtaaatatca tgtagtaaat tgttttgaac attctataaa
     6601 tgcattaaaa gaattagaaa tagtattaaa tgataaggat ttttttccaa tacatctaag
     6661 aaaacatgtg tcaaactatt taaaaacata tattgaagaa ggaataaata agttaaatgt
     6721 attaaagctg ggaacatttt tacatgatat aggtaagcct gacagtatga ccttagatga
     6781 aaccggaaga gttcatttca caaatcatga aaaaataggt gctcaaataa ttgacaatat
     6841 gggaataaag ttagagttat caacagagac atatagactt ataagtaaat atgtaagata
     6901 tcatatgata cttttgtctc tatataagaa aaatgatttg agtaggaaag aattaattaa
     6961 tgtatttaac ttagtagatg aggatactat aggtgtaata atgcttgggt atgcagatat
     7021 agtgtctaca gtaaaattat tgggcaagag tggagaagtc agtgtgttaa agacatatat
     7081 ggaatatatt ttaacaaatt acttgtataa gagtaattat acacatgtat aaatttagtt
     7141 aatataattg aattttagat gattaattga agtttgaaat atacggattt caaaaacata
     7201 aaagaagttg tctttgttgt aaaattatga aagacaactt tttttatatc aaaaattgaa
     7261 attttattaa ggatacttaa tggtctatta attaataaac cattagtatt taatttatat
     7321 tacaatattg attttgcttg tattggcgtt gatagtataa cagaggtctt agtagaacca
     7381 attttcttta tagtatcaat cacattttct agctcgtaca tatcttttac aattactttt
     7441 aaaagtaagc aatcatcacc tgttatatgg tgacattcta caatcctagg gtcctttgca
     7501 gctgactcaa taaattctgt atatccattg cttggaagag aaatatgaat aaatgccttt
     7561 ataactctgc ctaatgaatc tgggttgaca atagctttat atccttctat aacaccagac
     7621 tcttctaatc ttttgactct ttctgaaact gcaggagaag ttaaaccaac tatttttcct
     7681 aagtccttca tagaaattct cccatcatcc tgtaaaattt ctatgatcct gtaatctgta
     7741 acatccataa ataaacagtc ccccttaaat aaaaataatt aatgctataa aactttttta
     7801 gaatctatca tttgtgtttt taaataccaa agatttctag ataaatcaac cttataaatg
     7861 tgtggttttg aaagtctttt gtattgttcc cagatagtac ttaattcata ttgtacatga
     7921 ttttttattt caagaacagc ttttttatta tatttgcatc tgccttttat atacaatggt
     7981 ttaagaagct ctttaacttt gtaatttgta aataccttcg ttttccatgt ataagtagga
     8041 tggaagattt ctaaaggttt agactcatct ataacttcat catgtaacat aatcaaatct
     8101 gcctctgcct tattgtcttc gttatatatt cttataactt tcttgtaacc aggattattt
     8161 attttttctg gattctcaga aatctttatc ttaggttcaa gaactccttt ttcacaagat
     8221 gcagctaatt tataaactcc tccaagagaa ggtgaatcga aagatgtaat gagctttgta
     8281 cctactcccc atgaatttat agtagctcct tctgctttaa gtgatgttat ggtatattcg
     8341 tctaggtcat tagatgcagt gatgctgata tctgaaaagc cagcattatc taattgtttt
     8401 ttagcttctt tagataaata agctaaatct ccagagtcaa gtcttatacc cataggtttg
     8461 tatccttttt cagaaatatt tttgaatact tttattgcat taggaactcc actgtttaat
     8521 acatcataag tatctacaag tagtaggcat ttatctggat aaacatcagc atatgcttgg
     8581 aatgcttcaa gttcattatc aaatttttgt acccaactgt gagcttgtgt accaattata
     8641 ggtatatcaa acattttccc tgctagtacg tttgcagttc cagcacaacc accaactacg
     8701 gcagccctag ccccatatat ccctgcatct ggaccttgtg cacgacgtaa tccaaattca
     8761 aatacagggt ctccttgagc tgcaaagcaa actctagatg ctttagtagc aataagagac
     8821 tgaaaattaa ctattgttaa caatgctgtt tcaataagtt gagcttgata taaaggtgcc
     8881 ttaacagtaa ttaatggctc attagggaac atgatagttc cttcctcaac agcatatata
     8941 tctcctgtga atttaaattc ttttaaaaat tttaagaact tgtcagaaaa taaatttaag
     9001 ttttttaagt actctaaatc ttcatctgaa aaatgaagat tatctatgta ttcaacaacc
     9061 tgatcaatac cacaaactat agtataccct ccatcacaag cgttttttct aaaaaacata
     9121 tcaaatacaa caatgtcttc atgaatattc ttttcaaaat agccatttaa catagtaagc
     9181 tgatataaat cagtaagaag tgttagattt ctcattattt ctctccttaa ttattattaa
     9241 atattaaaaa ttgtaaaaac tcccacactt atactaaaac ataatatagt acaactttca
     9301 agtataaagt attacctaac tatcataaaa ttatttaaag aggaggatga ttataatagg
     9361 aaaaatatgg ttttatatgg tatcgatagg aataatagga agcttgattt ctaaaaatat
     9421 gggtgaatta aataaagtta tattaagtga aacatcaaaa ggtatagagt ttgcaataag
     9481 tctagctggt atcatggcac tctggatggg gataatgaat atcgcaaaag aatcaggttt
     9541 aattgaaaag ataggtcaaa aactaaatcc tataatgaga agattatttc cttctattcc
     9601 aaaaggtcat aaagcaatgt cttatatagt tatgaatatt gctttaaata tgttgggtgc
     9661 tggaaatggt gcaacagcat ttggtttaaa agcaatgagt gagcttcaaa ctttgaataa
     9721 caagaaagat acagccacta atgatatgat tatgtttatg gtaatcaata tatcatctat
     9781 acaaataatt ccatttacta taatcaaatt aaggatggat atgggggctc aaaatccaag
     9841 tgaaataatt tttacgactt tatttgcaac cataatatct acagcagtag caataataac
     9901 atgtaaattt tttcaaagaa gatatagata aaggggatgt agatacatgg agatattttc
     9961 aaacttactt ataccaataa taattctgta catagtaatt tatggtaaat ataaaaaaat
    10021 agatgtgtat gatagttttg taaaaggagc aattgatgga ttaaaagctg catgggatat
    10081 cctaccatat ataataggta tatttttggc aataggaata ttcaaaactg gtaaaggtct
    10141 tgatatgtta gaatggctat ttacaccaat agcaaatatg atgagtatac caaaagaatt
    10201 aataggtcta ataagtgtaa aacctttatc tggaagtggt gctttaggga tgtacagtga
    10261 acttgcaaat agagttggaa taggaagttt agtagaaaaa atgggtgcta cgattgtagg
    10321 ttcttctgag actatttttt acacaatggc aatatactat ggaagtctga agataaaaaa
    10381 tactagacat acattatcat gtgctatgat tagccatgtt gcaggtgtca tagcagctgt
    10441 ttttatttgc tatgtaatat ttgtataatt tgttgacaat atgggaaaaa ttttataata
    10501 tagtgattat atagaaacaa atagtaatac aaattacaag ctatgaaaag aagagtaaat
    10561 aaaggttttt tctatcagag agctaggtta ggtgaaagct agtgtcaaaa gctttattga
    10621 agatggtctt ggagcttttt atctgagcaa gtgtgctagg gtaaaacggt tggacacgtt
    10681 atagtctact aaggtatcta taaaatgttt ctacatttca tgtgaaacat aactattata
    10741 gatgaattag ggtggtaccg tgaataatct cgcccctatg ttaactcata ggaggcgatt
    10801 ttttattatt taaatgaaag aatcatatga ttacgaacga taaaaaatta ggaggtaaat
    10861 aacatgagca aaccgagttt ttatgtaaca acaccaatat actatccaag tggaggatta
    10921 cacataggac atacttattc aacagtagct gcagatacaa tagctagatt caagcgtttc
    10981 tgtggatatg atgtaaagtt cttaacagga acagatgagc atggtgaaaa aattcaaaag
    11041 aaagccatag aacaaggaat gtcagagata gagtatcttg atggaatgat aaaagatata
    11101 aaggccttat ggaatactat ggatatatct tatgatgatt ttataagaac tacagaaaaa
    11161 agacatacag acataataca aaagatattt actaaactat atgagcaagg tgatatatat
    11221 aaaggtgaat atgaaggtag atactgtact ccttgtgaga gtttctggac agaatctcaa
    11281 ttactagaag gaaataaatg ccctgactgt ggaagagaaa catatttagt aaaagaagaa
    11341 tcttatttct ttagattatc taaatatgaa gacagattaa aagagttatt taaagatgat
    11401 agtttctgtt ttcctgctgc tagaaaaaat gaaatggtgg caaacttttt agacaaagga
    11461 ttagaagatt taagcgtaac tagaacgact tttgactggg gtataaaagt tccttttgat
    11521 gaaaaacatg taatttatgt gtgggtagat gctttatgta actatataac agcattaggt
    11581 tatatgactg ataatgatga agaatttaaa aaatattggc ctgccaatgt tcaaatagta
    11641 ggtaaagaaa tagtgagatt ccatacaata atatggccag cgcttttaat ggctttaggt
    11701 ctagaagttc caaaacaagt atttggacat ggatggatac tatttgctga tgataaaatg
    11761 agtaaatcaa agggtaatgt ggtttatcca gaacctataa tagaaagata tggaatagac
    11821 acacttaagt acttcttatt aagagagttt gcatttgggc aagatggaag ttatactcat
    11881 agaaattttg taacaagaat aaattatgat ttagcaaatg atttaggaaa cttaataagt
    11941 agaactgtag ctatggttga aaaatacaat aatggtataa taccaacagc aaaagtttca
    12001 actgattttg atgctgactt aaaagaacaa gctgtttcaa ctagagaaaa ctttgaagct
    12061 gaaatggata aaatgcaatt ccatgaagca ttagagagtg tatggaaatt agttagaaga
    12121 actaacaaat atatagatga aactatgcct tgggcactag ctaaagatga aacgaagaaa
    12181 ggtgaattag acacagtttt atataattta tgtgaatcta taagaataat cgcaacacta
    12241 ataaatccta taatgaatga gactgctaat aaaatatatg aacatatagg aataaaaggt
    12301 caagatgata taactacatg ggaaagtaca aaaacatttg gtcttattgg agaaaatgta
    12361 aaagtattta aaggtgaacc tttattccca agattagatg tagaaaaaga gatagaagaa
    12421 ttaactaaaa tgttctctgg gaaacctcca gtagaagaaa aacctttaga gcataaagaa
    12481 gaaattacta ttgatgattt agacaaaata gagcttagag tagggaaaat aataagctgt
    12541 gaaaaacatc caaaagcaaa taaattatta gtatcacaag ttaagattgg accagaaaca
    12601 agacaaatag tatcaggaat agctgaatac tataagccag aggatttagt aggaaaagag
    12661 gttacagtgg tatgtaactt aaaaccagtt aaattaagag gtgtagaatc tcaaggtatg
    12721 atattagcag caggtgatga tggagaacca tatgttttac catttacaca aggggctaaa
    12781 gatggatgcg aggttcgtta gtaggtaatt aaatagtaag gagattaaag atgttatttg
    12841 actcacatgc acatttaaat gatgaaagtt ttgatgaaga tagagatgaa cttattggtt
    12901 cattaaaaga taaaggtgta gatttggttg taaatccagg agcagatata gagacgtcga
    12961 taacagcaat tgaacttgcg aaaaaatacg actttatata ttcagcagtt ggggtacatc
    13021 cacatgatgt atcaaaacta gatgatacag caatagaaac tctaagaaaa cttgcaactg
    13081 agaatgaaaa agttgtagct attggagaga ttggattaga ttattattat gattattctc
    13141 caagagaaga acaaaaagag tggtttaaga agcaaattga attagctaat gagctaaaac
    13201 ttccaataat aatacatgat agagatgcac atggagatac ttttgagata ataaagaata
    13261 ctaaaaatcc tgaaatagga tgtgtacttc attgttatag tgggaatgta gagttagcta
    13321 gagaatatgt taagatgggt tgttatatat caattccagg tacggttact tttaagaata
    13381 ataaaaaaac tagagaagta gtaagagaaa tacctctaga aagattattc atagagactg
    13441 attcaccata tatgtcacca gagccacata gaggaaagag aaacaatcct tctcaagtat
    13501 cttttgttgc tgataagata gcccaggaaa aagggatatc ttatgaagaa gtatgtagag
    13561 ttacaaaaga aaatgcaaag aaatttttta atataaaata gacaaattaa atcttataat
    13621 gatatatata ataagtcatt ataagtatag gatgagagtg cctcaaaatg agttttttag
    13681 tttatgaggc actctttttg tatgaaatca aatatatttt catcatttca acaatgaaaa
    13741 agaagtttat catattttga gatagtttgt ttttatttat ttaattcata ttttaaaata
    13801 tattttctta acattgttaa aaaagaattt acaagatatt cattagaatt aactcaactc
    13861 taatattcac ttaacttaag ttgtttatta tattattaaa gagaagtaaa cgaaaagtag
    13921 attaaggagg ttgaattttg tatacagttg atagtactaa gatattgata aatatgaact
    13981 tagagaaact agaaaagata tactcaatga taaaagatac atcaagtatt tctataatta
    14041 aagaacctaa cttggctaca gtaatggtaa gagctaatga aagtgtaaaa aacacaacat
    14101 ttaatttagg agagatatta gttactgaat gtagtgtaaa ggtagatgaa agcttgggtt
    14161 acggaatagt ttcagaaaac aataataaga aagccattta cttagcagtt atagatgcag
    14221 ttttacatag caataacagt aaatttgatg agttaaagaa ttacataaat aaaagtgtat
    14281 atgaagagaa cttaaggtat gaacaagaga ttattgatga atttagcttg attaataaaa
    14341 cgaaagttca attcaatgct atggattaaa taggaggaga atcatggtaa attatacaca
    14401 agaaatatat agaaagatgc tagatagtgc ttcatttcct gggaaattaa ataatataga
    14461 taatataaat atagaaaata atacaaaact attgaatgga gcaattggta tagcaatcac
    14521 tttattagac caagaagtta ccttctatat agaaaattat actcaaaaag atataaaaaa
    14581 tataagagca cttacaatgt caaatcaaat agattataga aaatcagatt atatattttt
    14641 agatattaat tcagaagtgg atatattaca gattaaaata ggttcttttg aatatcctga
    14701 tgaaagtgca actatagtac atcaagtaaa tgatttatca acgtactctc aaactaagta
    14761 tataaagctt gatttgagtg gtcctggtat aaaatctaaa aatagtatat atataaatgg
    14821 aattagagaa gaatttatag aaagattatc tagcataaat aaagattatc caataggaat
    14881 agatttgatt ttagtagata aaaaaggaga aattgcattt ataccaaggt catcaaaatt
    14941 aagttgggag gtattataga aatggcttat gtagcagtta aaggtggaga agaagccata
    15001 ttacaggcta aaaatatact tgagttttat agggtaaaag gagatagctt gcctatagat
    15061 gtaaagcaga ttgaggaaca aatgtcttat gctgtagaca aagtaatgtc tgaaggaagt
    15121 ttatacaaca aggaactatc agcattggct ataaaacaag ctcaaggtga tatattagaa
    15181 gcctctttct atgtaagagc tttaaggtca acaatgccta gaattacata ttcagaaccc
    15241 ataaatacag aagaaatgag aataaaaaga agaatatcag ctacttttaa agatatacca
    15301 ggaggtcagt atcttggggc aactagagac tatgaaaata gaatattgaa tgtagaatta
    15361 ttagaagaag atgaaaaaaa gaatagagat tttagagata gttttataag tgaattagaa
    15421 acagtagatg aaatccctga atttacaaaa atatctaata tatttagaaa agaaaattta
    15481 ttacaagaag aagtttgtga aaacaaattg gaagaagtta ttgatgatat tacaagaaat
    15541 agtttaagat ttccagtttc taggtcagca aagttacagg cattaactag aggtgaaagt
    15601 ggagtagtgt caggttttgc ttatgctatt gtaagaggtt ttggtgatga acatccatat
    15661 ataagtgaac ttagaactgg taatgtattt ataaaagtaa aacatcccat ctatgaagat
    15721 gaagaaataa taataggtga tatgctggtt actgaatgtg aaatagtttc aaaagcatca
    15781 caaaataaga ctgaaaattc taaaactaat tcggaagata aaaataagat gtctttagga
    15841 tatggtcttt gtatggggaa taatgagaat aaggctatat ctatggccat actagacaaa
    15901 tctttggagc caaatagtga aaaactagca caagatgaag aatttgtatt gcttcatata
    15961 gatggaatag attcattagg atttatatct cactttaagc ttcctcacta tgtagatttt
    16021 aaagcggatg tagaaagaat aaaatagttt gtggaggtaa gtttaggtga tatataacta
    16081 tggtttttta gacgaaaaca caaaaaaaga aataagaaga aagatattaa aagcaatatc
    16141 aatacctggt tatcaagttc cctttggttc aagagagttg cctatagcta agggatgggg
    16201 tactggaggt ttacaattaa cactatcatt aattggtaaa aatgatgtag taaaagtaat
    16261 tgaccaagga agtgatgatt ctacaaatgc ttgtaatata aggagcttta ttgcaagtgt
    16321 aagtaatgta gaaaccacaa aagatacatt agaagctaca ttgattcaaa cgagacatag
    16381 aataccagaa gaaaaactta aatctaatca aatactaata tttcaagtac caataccaga
    16441 aacccttaga atagtagaac ctagtgaagt agaaacaaga agaatgcatt cagaagaaga
    16501 ttatagtagg atgtgggtat atctttatga agatatagtt agatttaatg atatatctat
    16561 agcagttgaa tatccatgta aagtaaatga tagatattta atgaatccat cacctatacc
    16621 tagatttgat ataaaaaaat taaatatgtc agacaattta ttcttgtttg gtgcaggtag
    16681 agaaaaaaga atttatgcta ttcctccata tacaaaggta gaacctttag agtttgagga
    16741 ttataagttt gaagaggaaa agtttgaagg aaaatactgt agtttatgta acagtacaaa
    16801 tacattttta gatgaagtat atgacagtga cactaatgaa aagtactata gttgctcaga
    16861 tacaagttat tgtgaaaaag ttagattaaa aaataacagt atagatgtga caataggagg
    16921 tacttggaat gagtaaaata aatgactcta aaagattaga aaatatagac aagcaagtat
    16981 taataggaag aaatatcaat cttatttatg gagatgggtg tgaaaagtgt tttgaaagca
    17041 caggatatga aagcaataat atatgtaagt attgtggttc tatagttgca tgtaataatg
    17101 taaatttaga actttatgaa ggagaagtcc ttggaattgt aggtgaatct ggttcaggaa
    17161 aaagcacttt attaaaaata ctgtttttcc aagaacatgc aaatagtgga gaagtatata
    17221 tatcccatta caataaagat acaaatatac tagagctttc agaccaaaaa aagagataca
    17281 taaagaatca ttttatgggt attgtgtatc aaaatcccca ccttggttta aatttagact
    17341 ttagtagtgg tggtaatata gcagaaaaat tattgatggc aaatttatat aatgttgaaa
    17401 aaataagaga tagaggcaaa gaacttttaa taaaaactaa tatacctgta gaaagaatag
    17461 accataaacc aaaatacttt agtgggggaa tgcaacaacg agttcaaata gcaaaagctc
    17521 tttcaaataa ccctccaata ttgctattag atgaggttac aacaggttta gatgtttctg
    17581 tgcaagcgga agtattggat ttaataagag aaatccaaag agaacttaag atttctatga
    17641 tagttgtatc ccatgacttt gatgttataa agatgcttgc tgataggaca attgtaatga
    17701 aaaatggaag tgtaattgag tctggtctta cagaccaaat aatggaggac ccacaacacc
    17761 catatactca gcaacttata aattcattgc tataaatatc gtttgtaaat aagcaaaatg
    17821 ggggttttta ataaaatgaa agaaactata cttgaagtaa ataattttag taaagacttt
    17881 gagttacatg cattgaacaa gactataacg gcatgtagta atataagttt tgaagtttca
    17941 aaaggtgagt ttttaggaat aataggtaaa tctggagcag gtaagtctac catattaaaa
    18001 agtatctata aaacatatat accaacaact ggagaaataa tttttaattc agaaatatat
    18061 ggtaatgtag acctggctaa aattggagac cgagaaatga taaatcttag aaagaaagaa
    18121 ataggatatg tatcacagtt tttaaagaca cttccacgaa taactgcaat tgaactggta
    18181 gtacattctt taattgagag tgggtttgaa aaagattgtt cgtatgatat ggcaaaagat
    18241 atacttaacc aatttgaaat aaaggaaaat ttatgggatg cgtatccaaa taatttttct
    18301 ggtggagaaa aattaagatt aaatttagct caagctatgg ttaaaaagcc tagacttttg
    18361 ttacttgatg aaccaactgc atcacttgat aatcaatcta aaatatatgt aaaagaaaaa
    18421 atgttggagc ttaaaagtca aggaactaca atgataggaa tttttcatga tatagagttt
    18481 atggaaactg ttatagataa gacctttact atggcaaaag ggactataag tgaaagtggg
    18541 gtagcctaga tgataaaact aattcataat gcaaatattg tattagaaga tatgatttta
    18601 gaaaatggtc atttaatcat agagggagat acaataaaaa aagtatcaaa tgataagatt
    18661 gattacgcat ttgactttaa tgagatagat gaaataatag atgcaaaaaa cttatatgta
    18721 atacctggta ttatagatat tcacagtgat gccattgaaa aggaaataga gccgagacca
    18781 agtacattat taccatttaa tatggcattt tatgaactag ataaaaaact acctgcaaat
    18841 ggaataacta cagtttatca ttctatatct ttaggggatg gagtaggagt tagaagtatt
    18901 gataactctt taaagatgat taaaaatatt gattcatata aaaatataga tagtaagagc
    18961 ataaatcata aagttcattt aagatatgaa gtgttgtatt atgaaggttt agaaaaggtt
    19021 ttagaactat tagatgaaaa taaaatagac tacttatcaa taatggacca ttctccagga
    19081 caaggtcagt atacaaatcc aacattttat aaagagtatg ctactaaagt gtggggagtt
    19141 acagaaaatt atgtagatac atggttagat gatttagtaa atctgcatga taatttagat
    19201 tggaataaaa tagctaatat tataggtata gccaaaacta aaaatataaa tgttgcatca
    19261 catgatgatg atacattaga aaaaatggat ttcataaaaa atataggcat aaatgtatct
    19321 gaatttccta taactcttga agttgcaaaa cattcaaaga aattgggtgt tctaacttgt
    19381 ttaggtgcac cgaatattgt aagaggtaaa tctcataata acaatttaaa agcaatggat
    19441 gctatattgg aagattgttg tgacattgtg tgttcggatt atcttccatc agctatgata
    19501 aagtctatgt gtatagttgc agaaagaata aataatttaa ataaagcagt cagtttattt
    19561 acctcaaatc ctgctaaagc agttggtata tatgatgaaa gaggtagtat aaaagaaaat
    19621 aaaaaagcag atttagtttt agtagatatt gatagtgaat atccaaaggt tgtaaatact
    19681 attgtaaatg gaaaaacagt ttataaaagg gaggtataaa gattgctaac agagttacat
    19741 tgtcatacaa gtatatcaga taataatttt actactgaag aaataataaa aaaagcagca
    19801 gaaaagaata taaatatctt agcaataaca aatcatgaca cattatctgg attggatgaa
    19861 gctcttgtgc ttggagagca ttatggaata acaataatac caggtataga aatttcagcc
    19921 tatgattata aaaatagaaa aagagttcat attcttggat ataacataga tttaaacagt
    19981 caagaaataa aaaatttgtg caatccaatg gtatgtgaca gacataaagc atctgttaag
    20041 atggtaaata aaattataga tttaggatac aaaatatctc tggaagatgt aaagaaatat
    20101 tcatcaaaga ctggaatatt taaacaacat ataatgaaag ctcttataga taaaggatat
    20161 acagataata tatattcaaa tctatataaa gaattgtttc atagaggaaa cggcaaggta
    20221 tatgtatctt taaaatatgt ggattacaga gatgcaataa aagctataaa aaattctggt
    20281 ggaatttgtg tgttagctca cccgggtcaa atggataatt ttagcgctat tgaagagatg
    20341 gtaaaagtag gattagatgg aatagaagta tatcatccat ctcacaacaa agaactagaa
    20401 aaagagtcgc tttcttatgc taagaagtat aatctagtgg tcacaggagg ttcagattac
    20461 cacggatttt attctcaaca taattatgaa cttggaagca agagcttaaa taaagaagat
    20521 ttatttaagt ttaggttagc tttaggaggg gtattataaa tgtctttttt acaagtaaaa
    20581 aatgtaggta aaagttatgg acaagttaag gtgttaaaag acatatctat tgatattgaa
    20641 aaaggagagt ttatttgttt actaggtcca agtggttgtg gaaaaagtac actacttaga
    20701 ataattgcag ggcttgagga taaacatggt ggaaaaataa taataaatga taaggatatg
    20761 actaattcac cacctgaaag tagaaatttt ggtatagttt ttcaatcata tgcattattt
    20821 ccaaatatga atgtttacaa aaacatagcc tttggactag aaaataaaaa cataagtaaa
    20881 tctaatattg ataaaaaagt taaagaagtt ctagaagttg tagaattaag tggatatgag
    20941 aaaaaatatc catcacaatt atcaggtgga caacaacaaa gggttgcatt agctcgtgcg
    21001 attgctttag agccagactt tttacttctt gatgaacctt tatctgcatt agatgcaaaa
    21061 gtaagactta aacttaggga gcagattaga agtttacata gaaaactagg cattacaact
    21121 ataatggtaa cgcatgacca agaagaagct ctttgtttag ctgataagat ggttgttatg
    21181 aatagaggag aaataataca agttggaacg ccaaaggagg tatataaaaa tccagaaaca
    21241 ccttttgtag ctgattttat aggaacaata aattttatag atgatggtat taataagata
    21301 gctattagac cagaagatat aaaagtagag tctaatagag attctaaaga taaggatata
    21361 aaagtgggag aaattttaga tatcgaattt agaggattta actatagaat tacagttgag
    21421 tatcgttcta agcaaatgaa attagatgta gtttccaaag ttgcagaaca aatgaaactg
    21481 tgcataggtt ctaaaataaa ctttaagata cctaaagaag gaattgtaca atataaatca
    21541 gagggatgtg cttaatagct aggggtgaat aaatgataac atcaaaaaat aaaagaagaa
    21601 taagtgatag cacaatacaa aaatatttct tgattttttc ggtaattgta ctaatagtat
    21661 ttatattatt tccattagta tcattattaa aaaatgcatt tatggataca agtggaaatt
    21721 ttataggaat ttcaaacttt agtaaatatg ttgaaaatcc aagtcttata gcatcattta
    21781 aaaattcaat ttttgtatct acagtctcat ctttaatatc actatgttta gcatttatat
    21841 attcatacgc aataacaaga acaaacataa agtttaaaga tttttttaaa actatgggaa
    21901 tgtttccact ttttgcacca actatgttat atggtatttc acttatatac ctttttggaa
    21961 ataagggaat ctttacaacc atgggtctgg aagtaccact gtatggtcca ttaggaataa
    22021 ttatttctca gattctattt acatttccac aagctttttt aatattatct gtagcacttt
    22081 cgatggctga ttacagactt tatgaagcag cagatagctt gggtgctagt gaatttaaga
    22141 aattttgcaa aataacaatt cctggtataa agtactcatt acttagttca ttctttgtat
    22201 cctttatact tgcatttaca gattttggag cagctaaggt agtcggagga aactacaatg
    22261 tactggctac tgacatatat aaacaagtag ttggacaatt taacatacca atgggagcta
    22321 ctgtttctat ggtaatgtta attcctgtac taatagcatt tacattagat aagatggcaa
    22381 gtaagaaaca aggtatgacc ataactgcaa agtctatgcc atataagatt aaggaaaata
    22441 agtttagaga tagagtattt ttaatagtat gtagttttat aacattgcta atttttattt
    22501 taatattcgt ttcagttata ggttcagtta taaagttatg gccatataat ttaagcttta
    22561 caatggaaca ctatacattt aaaggtattt tgggaagtgg aataaaaaca tacttaagca
    22621 gtttgaaaat atcaacttta acagcaatat ttggaactat atttgtattt tttagtgctt
    22681 atatgataga aaaggtggat aagtttccta aattaagaca aacagcttac cttttatcaa
    22741 tgataccaat ggccttacct ggcttagttt taggtatatc atatataact ttttttaact
    22801 cagcaagtaa tccattaaat tttttatatg gaagtacagc aatattagtt atagtaaatg
    22861 tagttcattt ttattcagtt tgttttataa cctcaaattc atctttaaaa atgttggata
    22921 aagagtatga attagttgca aaatccataa atattccatt ttataaagta ttttttaata
    22981 taacagttcc aatgtctata acatcaatac ttgaaatagt agtatattat tttgtaaatt
    23041 caatggttac tgtttcagca ttaatatttt tatatacacc tcaaactcag actgcatcaa
    23101 tatcaatact aaagttagat gagataggat atataggacc atcagctgct atggcagtat
    23161 tagtgctttt aacaaatata atagtaagac ttttgtacga atttgtaaca aagaaattaa
    23221 aaaataacac acaaaaatgg cagcaaaaag atgttgcata ggggaggtaa aaatgaaatt
    23281 taagaagcta gtttctttag gagtattatc aacactttta ttaagtgtaa taacaggatg
    23341 tagtactgat tcatcaggtt cagattcaaa gtctgatagt aagacaaaag gggatttaac
    23401 agtttacaca gcaattgaag aagatagtat agagccatat ttagctacat ttaaagagaa
    23461 atatccagat attaaattaa atattgttag ggcatcaaca ggagatataa cagcaagatt
    23521 attagcagaa aaggataatc ctcaagcaga tgttgtatgg ggagttgcag ccacaagctt
    23581 attggttgca gatgaccagg ggatgttaga accatatgct ccaaaaggtt ctgaagaaat
    23641 agagtctgca tttaaagatg ataaagaagt tccttcttgg gttgggatag atgcttggat
    23701 gacaggtata acagtaaaca ctaaagaatt aagtgataag aacatcccag ttcctagttc
    23761 ttacgaagat ttaataaagc cagaatataa aggtcttata tctatgccta atccatcttc
    23821 ttctggtaca gggtacttaa cagtatcagc ccttatacaa attatgggtg aagaaaaagc
    23881 atggcagtat atggataaat tgcatgagaa tattggtgta tacactcaat ctggttcagc
    23941 tccagctaca tcagcagctt caggagaata tcctattggt atatcttttg gatatagagg
    24001 tataaagtta aaagaggaag gctatccagt agaagtagta tttccaaaag aaggttctgg
    24061 atgggatata gaagcaaatg ctcttgtgaa aaaagataat ataaaagaag catcaaaagt
    24121 atttttagat tgggctataa gtaaagatgc aatgaacgaa tactctaaga actatgcagt
    24181 tactactatt tctacaggaa atccaatacc agaaggattc cctaaaaagc cattagagca
    24241 aatgatagat aatgatttaa aatcagcagc aaaaaataga gaagatatac taaataaatg
    24301 gatatcaaaa tatgatggta agacagaaaa agagagttaa gtaaaactat acaaatggag
    24361 taaaaagaaa gaattttttt aagtagattt tattataata atataagttg agaataatat
    24421 aggtcaagaa aagtgaatat tctataaaaa tgtatagagt aagtatacca ttcagtttga
    24481 atggtatatt ttttattttt agagattaat ttagaaatag atatactaca gtttaaaata
    24541 gtatatttta atttcattat taaagtatag atattatata taaagtaaat aaattattga
    24601 gcgattatta ataagatatg tataaatttt aatttgggaa cttgtgttat atatcatgaa
    24661 aaaagagaaa aaataaaagg taaattgttt cttgataact tgaaattgga agtgagaata
    24721 tttaactaga gaagaattat ttaagtttaa gtcagcttta ggaggaggaa ttataaatgt
    24781 cttttttaca agtaaaaaat gtaggtaaaa gttatggaca agttaaggtg ctaaaagaca
    24841 tatctattga tattgaaaaa ggggagttta tttgcttatt aggtccaagt ggatactata
    24901 tgttagtcca aataattata taaaattaat cgtgatgttt ttgtcattat ctatttctat
    24961 ataatctatt atagataacc aaaaacgtct gcgttcaacc ctgcttaaat cactatacat
    25021 ctcattaata tcaatatcta agaatttttt tatatgtgat gtatcaataa tttgactctc
    25081 ctgttcttca gttatattat ccagttgaga ttgtaatttt tcataatctt ttctataagt
    25141 ctctttatca atcaaatcct ctaaatataa atctttgatt ttatctaatt ttttcataat
    25201 tttcttcttt tcatcttttt tgttagattt tttattttgt ctgatttttt cattttgtgc
    25261 ttctaatttc accatatgtt tatgtaattg tggttttaag ttagtcaaaa ggtagttttc
    25321 tatatgtttt tcattatgag ttacattgtg tacacagttt ttatttagat agaatgtaga
    25381 acatctataa gataaataaa gtttttctcc aacattacta gaattaccct ttaatgtata
    25441 accacactcc ttacatttta aaagaccaga aaaaatatga taatgtatgt ttttattagg
    25501 agtagttttt ttattttttt ctaatattct ttggatttgt ttaaattgtt tttttgatat
    25561 aataggttca caataatctt caacataaaa tcctcgttta ttatagattc ctatataaag
    25621 ttcatttctt agtatattct ttattttatt ataactgtat aattcgccat gaagattatt
    25681 aattgtttct attaaagtcc taacagaacc tgatttctcg tattcgtcaa atatagcttt
    25741 aactatatat tttttttctt tatcaataac tagatgctta ttttctattt tgtaacctat
    25801 aggaattgta ccgcttataa cttctttacg tctcaattta tcctgaaaca caaattttat
    25861 tctctcagat gtttgagctg cttcattttc tgctacagat aacataatgt ttatatgtaa
    25921 tctaccagca gcagtactag aatcatagtt ttctaaaatg gttttccaat cacatttatg
    25981 tttttcaaaa gtctccatta ttttataata gtttttaacg ccacgactta gtctatcaat
    26041 tttagtcatt aaaactaggt ctactttatc tttttccaca tctcttaata atcgttgtaa
    26101 gttaggtctt tttaaattag ttgcagagca accttcatca atgtatttac caactatgtt
    26161 aaaatcattc tctttagagt actgttctaa agcctctatt tgtgttctta tgctatctcc
    26221 atttaaagct tgttcttctg tactaacacg tatataaagt gctactctta acattttaca
    26281 ttccccctta ttgtatattt aagaggagct gaacggctct taaatatact atgaattgtt
    26341 acaaaaaatt tgcattcata atactaaatc ttggctcaaa aaatatcaca taattatcta
    26401 gtgtagctga aaaaccatat ttacttttat aacattctaa agcttcattt agaaactttt
    26461 cagttacatc aaggtattct gccatttcgg ttatagtctt acagccagca ttaaaactat
    26521 ctattatacc tttaagacca attaacttgt tgtaagccat caatctagct ttatattctt
    26581 gtttgctatt ttcaacttta tttaggtcta atatatttcc atatgaagta taatgatgcc
    26641 ctagttcttc tgctaacaca caagcttttt cactcatatt attaagtgta tttttattta
    26701 aagctattct attattttta tataatccat gagaatttga aattaaagaa acttctctaa
    26761 caataatatt attatcatct gcttcagaga gtaacttttc ataactattc ataagttcac
    26821 ctacctattt taccaattat ccatatcatc taaatcttga tatatttttt caatttctcc
    26881 ttcttcatgt aaatgttcat tgtgtgccgc tattgtgtct atgtgatttt tatttacata
    26941 tttacctatt tgagtaagtt catctactct ttttattgct tcattttgtc caatatcgtt
    27001 taaggcatta aagcttgata aaagcttatt ttctttaatt ttaatttgtt tgcagtcttc
    27061 gttccacccc attataaaag caggagattc atctaatgca tgtgctaaaa tttctagctt
    27121 atcaagagga atatttttta tagctcccgt ttcatatctc tgtaaagtgg acttacttag
    27181 tccagtcttg ttagctaaat cttgataaga catattaagt tctaatcgtt tattttttat
    27241 tctatttgta atctccaaca tttttaaatc attttcagta ttcaaattta tcacctctat
    27301 aaaatatatt ataaagtatt ttttcataaa tgcaacacat atattggaaa atataaaaaa
    27361 atgttgcatt tttgacttga caatataaaa tggatgtatt aatattgaat tatcccaaat
    27421 aagcaacgag gtgatggaat gaatataagt aaattaaaag gaaaaatggt cgaaaagaaa
    27481 tacactcaaa agaaattagc agaagagttg ggtatgactg ttcaagcttt aaatgcaaaa
    27541 ttaaataatc gttcacaatt caagattgaa gaagcagtta agatatcaaa tattttgcaa
    27601 atcgaagagc ctagtgagat tttttttgac aattatatcc caaaaatgca acgaaattaa
    27661 ataagaaaac ctaacagata aaaattaaaa ctagggaggt agaaattatg agtaataatt
    27721 tacaagtaat agaaagaaac aacaaaagag ttctaactac acagcaacta gcagatgtat
    27781 atgaaactga ctcaaagaat ataagtaaca atttcaacaa taacaaagat aggtttattg
    27841 aaggtaaaca ttatttttta ttacaaggtg atgatttaaa gaattttaaa ggtattcata
    27901 cagaatatga aaacctaaaa tttgcttcaa aaatgtatct ttggactgaa agaggagcaa
    27961 ataggcactg caaaatatta gatactgata aagcttggga gcaatttgac aacttagagg
    28021 aaacatattt caaggttaaa caacagaaac caacctgtat agaagatgta ttaatagaaa
    28081 gtttaaaaga aatgaaagat ttaagacttc aagttaatca agctaataat attgcattag
    28141 aagcaaagac agaggttaaa acaataaaag aggttgtttc attgaatgct actgactgga
    28201 gaaaagatac acaacaacta attgcaagga tagcaaagaa acaaggtgga tttgaacata
    28261 taaatatgct tagaacagaa agttatgaat tattaaataa gcgattcgga gttgatttgc
    28321 atagaagatt aatcaataaa agaagaaaaa tggcagagga aggtgtatct gaatctaaaa
    28381 gagagaaagt taacaattta gatgtaatac aagatgataa gaagctaata gaggggtatg
    28441 tggctattgt aaaagatatg gctttaaaat atggaatatc aagtgattta agtaagaatt
    28501 aggttaaatc ataacagcac attgaaaact aaatataaaa ttttaaaagg agtaagtata
    28561 tgagaaataa agaaaaagag gctattataa atagtaatag tgctaagaag gtgattgaat
    28621 tgaaacacga gcaaacaacg cttcgtatac cagaggactt gtacaaagcg ttgatagatt
    28681 taagtaggga aatcggaatg cctattgcat ctattgtaat aattgcgtgt tggttgtata
    28741 tatcaaagat aaattaacca atgagatatg caaaatgaat gcacagcatt atttattaga
    28801 gaattgactg ataaattatt ttctttagct gttttctcta gcaactcttt tagaacacta
    28861 ggaatcctaa gagtagaacg aacgctatca tcacttttat acgagactga tttaagttca
    28921 ttaacttttg aatttctaat aatgtcatta atagcgtata gaataagcga agatttaaga
    28981 atacctgttt gacaagaaat tttttctaaa ttttcattta atggtgtata tattcttaca
    29041 gtgatagtag tcattttgac acctccttaa tgacattata caaaaaatat ttgaaaaagt
    29101 cttgacagta aatcggtgtc acaatataat ggatatagac agtaagacag tgtcataatg
    29161 aagggaggaa taagaatgga aaaaataaga atgactgtaa ggctcgtccc aaaattaaat
    29221 caatatgtac agaatatagc caaagaatgt ggaaaaagta aaaattctat tattgtggat
    29281 gcatgttggg agtttattga gaaaataaaa aaagaaaata aattcaatgt agaaagcgag
    29341 gaataaatat gaataatcta caaatattta aaaataatac ttttggtgaa ataagagtaa
    29401 tcgaaataaa agaagaacct tggtttgtcg gaaaagacgt agcagaaaaa ttaggatata
    29461 aagatacatc cgacgcatta aaaagacatg ttgatgatga agataagggg gtaggtgaaa
    29521 taccgactcc tggaggaaat caaaatatga ggttaattaa tgaaagtgga ctttattctt
    29581 taatattaag ttctaagtta ccaaatgcaa aagcattcaa acgttgggtg actaatgaga
    29641 tactgccaag cattcgtaag actggtggat atatacatac aactgaaaat atgtcagacg
    29701 acgaaataat ggcaagagca ttacaagtag ctcaaaagac aatagaaaag aaaagtagag
    29761 aaatagaaga aaaggataaa gtaattcagt tacaacaacc aaaagtactt tttgctgatg
    29821 cagtagcatc atcagatgat tctatattag ttggagaact tgcaaaacta cttaagcaaa
    29881 atggtattga tacaggcgag aaaagattat tcgcatggct tagagataat ggctacttaa
    29941 taaaacgtaa aggtgaggat tataatacac caactcaaaa gagtgttgac ttaaaaataa
    30001 tggaaactaa aaaaagagca atatataacc ctgacggaag tacaaagatt actaaaacac
    30061 caaaagtaac aggcaaaggt caaatatact ttgtcaacaa attgaaatca gtaaatcaaa
    30121 tatcaatatt aagctaaata aaaccagcac tttgaaaatt aaatacagaa tattttgaaa
    30181 atagaggggt gatttaaatg ggtaaaaaaa gtgaatacac agtaacaaca gttggtgaac
    30241 caaatttaga tgcattaggc gaaactttag ctaaaattct tacagagaaa tttggggtgc
    30301 taataactca tacatacatt gagaactatt atgttgataa agagagaggg ggtgatgtag
    30361 atggagctat tgaaagtgaa ctttaatcta gagaaattaa aaataggaaa tgtagtgagt
    30421 gtaagttcta agaggtttgg gttcaatatt aattgtattg tagtagtagc aactgaggag
    30481 gaactaaatt tagcttatta tagcaaagat aagggatgta ttgagtatca agcattgata
    30541 ccagaggata ttagatatgg tgattatatt cttgaaagat taggttagga ggaaataaaa
    30601 tggcagcttt aataatagta ggtttatttg caatatgttt agtaggatta gttcaaaata
    30661 gagattaaat taagggggat taattatgga aagtttaatt atagtaagaa atgcagtaga
    30721 gcaacaacta aatagagcta atttagaaat aaataaaaat gagcaacttt atacaaaact
    30781 tagaaaaaaa gaagaaagaa atatattaga agaaattgaa ttgagtaatg ctttaagaga
    30841 aaaaagtgca aacgaaagat taaaaatatt tgctgagtca ttactagagt tgttagatac
    30901 acaaatcaaa ataaaagaga atgaagaaag cgaggactat aaaagacttt gtatgtactt
    30961 agacgaattt ggaagagata gacctataga tgtccagata taaaaaaaga gccattagga
    31021 gtggctcgat tcagaaaata tttaaaaata aaaatatgac tatattatag cataaacgga
    31081 gggaaattat gagtacttta tatgaattaa ctacagattt attagaaata gaagaaggtt
    31141 taacagaaac aacaggaaat gaagctgaaa aactagagga aataaaagaa ataataaaac
    31201 aagagataca aaataaaaac actagaatca tatcagtaat aataaatatc gatagtgata
    31261 taaactctat agattcagag attaaaagat tgcaagagtt aaaaagggtc aaaaagaata
    31321 ctcttgatag attaaaaagc aatataaaag actgtatgga attacttggg actaaaaaag
    31381 tagaaacagt tttaggaaat ataagtataa gaaagtcagc aggtagctta gtcatagaag
    31441 atgaagaaaa gatacctgct atatataaaa cagtagagca agttataaaa gtagataaaa
    31501 atagcattaa agactttatt aaaaaaggtc atgaggttga aggttgcagg attgaatatg
    31561 gaactacatt aacaattcca aaagctaaaa aagagtaggt gggagatatg gaaactaata
    31621 atatttatat gaagcttgta aatatacaga gcgctttaaa agctcctaaa agtcaattta
    31681 atagctttgg taaatacaat tataggagtt gtgaggatat actagaaggt ttaaaaccta
    31741 ttctaaagga agaaaaagca ttagttatat tggatgataa tattgttcag ataggaaata
    31801 gattttatgt agaagctaca gcaactttaa tagatgcaga aacaggagag aaagtatcta
    31861 caaaggcatt agctagagaa gatgaaacta aaaaaggtat ggatttagca caagtaactg
    31921 gaagtgtatc aagttatgca agaaagtacg ctttaaatgg attattttgt attgatgata
    31981 caaaagatag tgatgcaaca aataaacatg gaaatgagca gaaaaaaaaa gaagttaatg
    32041 agagtgaatt aaatacacta tattc
//
LOCUS       DAEMHZ010000036        30321 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000036 DAEMHZ010000000
VERSION     DAEMHZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.036"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..1144
                     /locus_tag="KPK72_001536"
     CDS             32..1144
                     /locus_tag="KPK72_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG2488764.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLILKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KPK72_001537"
     CDS             1398..3422
                     /locus_tag="KPK72_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2488765.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KPK72_001538"
     CDS             3642..4328
                     /locus_tag="KPK72_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG2488766.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KPK72_001539"
     CDS             4423..5478
                     /locus_tag="KPK72_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG2488767.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKLYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="KPK72_001540"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="KPK72_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2488768.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="KPK72_001541"
     CDS             7195..8901
                     /locus_tag="KPK72_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2488769.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRVTLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKDYNVDCLNTLTGFKFIGEKIK
                     AFEENNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="KPK72_001542"
     CDS             8923..9996
                     /locus_tag="KPK72_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG2488770.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="KPK72_001543"
     CDS             10008..10730
                     /locus_tag="KPK72_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2488771.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="KPK72_001544"
     CDS             10746..11903
                     /locus_tag="KPK72_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG2488772.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGIVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="KPK72_001545"
     CDS             11906..12745
                     /locus_tag="KPK72_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2488773.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="KPK72_001546"
     CDS             12760..13935
                     /locus_tag="KPK72_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG2488774.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFNKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="KPK72_001547"
     CDS             13964..14713
                     /locus_tag="KPK72_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2488775.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="KPK72_001548"
     CDS             14735..15643
                     /locus_tag="KPK72_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2488776.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="KPK72_001549"
     CDS             15691..16443
                     /locus_tag="KPK72_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2488777.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16421..17764
                     /locus_tag="KPK72_001550"
     CDS             16421..17764
                     /locus_tag="KPK72_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG2488778.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDIDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17770..18861
                     /locus_tag="KPK72_001551"
     CDS             17770..18861
                     /locus_tag="KPK72_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2488779.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="KPK72_001552"
     CDS             18945..20858
                     /locus_tag="KPK72_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2488780.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="KPK72_001553"
     CDS             21057..21764
                     /locus_tag="KPK72_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2488781.1"
                     /translation="MMNIKNKKHILKKFIAMALIAGVVTIEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="KPK72_001554"
     CDS             22066..24177
                     /locus_tag="KPK72_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG2488782.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="KPK72_001555"
     CDS             complement(24375..25340)
                     /locus_tag="KPK72_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2488783.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIKFKQ"
     gene            complement(25625..26581)
                     /locus_tag="KPK72_001556"
     CDS             complement(25625..26581)
                     /locus_tag="KPK72_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2488784.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="KPK72_001557"
     CDS             26949..27737
                     /locus_tag="KPK72_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2488785.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKGLVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="KPK72_001558"
     CDS             complement(27860..28330)
                     /locus_tag="KPK72_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG2488786.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="KPK72_001559"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="KPK72_001559"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG2488787.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="KPK72_001560"
     CDS             29156..30061
                     /locus_tag="KPK72_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2488788.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12014 a         3298 c         5382 g         9627 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttaatact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact ttcttatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgaaata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagagtta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 tgaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccatagtaca aacttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggcttaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaattaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataat aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagtt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagctttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgtgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaagca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gctttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggat tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcttttgaag aaaacaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaagat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa taatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctaatct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgacccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcagca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattgat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttggg cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaataattt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccga
    11461 taatacttac tttatttagt gtaattattg gtatagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggcatgtata cttggctgaa aaggggaatt
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttaataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagaa tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tatagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagagcttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aaacataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagcgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttatgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taaaaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt gaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttgta tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggcg ttaatagcag
    21121 gagttgtcac aatagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggggt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttccggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaag aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaggaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattatgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcagctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta taaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaacttt atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctgtgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc acccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc attgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ttccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca cgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactgatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atcttataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa ggattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggtcagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaagg agacatattg aagatagctg tgtttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tatttttaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaagaagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat atctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 agatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatctc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactaatcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gggctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 agttaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgca aacattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat a
//
LOCUS       DAEMHZ010000037        30260 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000037 DAEMHZ010000000
VERSION     DAEMHZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.037"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..805)
                     /locus_tag="KPK72_001561"
     CDS             complement(<1..805)
                     /locus_tag="KPK72_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG2488789.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEEK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVE"
     gene            complement(858..1217)
                     /locus_tag="KPK72_001562"
     CDS             complement(858..1217)
                     /locus_tag="KPK72_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG2488790.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQENAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(1271..2356)
                     /locus_tag="KPK72_001563"
     CDS             complement(1271..2356)
                     /locus_tag="KPK72_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2488791.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNVKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(2527..3906)
                     /locus_tag="KPK72_001564"
     CDS             complement(2527..3906)
                     /locus_tag="KPK72_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2488792.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3899..4579)
                     /locus_tag="KPK72_001565"
     CDS             complement(3899..4579)
                     /locus_tag="KPK72_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2488793.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4970..6049)
                     /locus_tag="KPK72_001566"
     CDS             complement(4970..6049)
                     /locus_tag="KPK72_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2488794.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKNTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6337..6452
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6627..7997
                     /locus_tag="KPK72_001567"
     CDS             6627..7997
                     /locus_tag="KPK72_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2488795.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8262..8873)
                     /locus_tag="KPK72_001568"
     CDS             complement(8262..8873)
                     /locus_tag="KPK72_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG2488796.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8863..11226)
                     /gene="lon"
                     /locus_tag="KPK72_001569"
     CDS             complement(8863..11226)
                     /gene="lon"
                     /locus_tag="KPK72_001569"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG2488797.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDSEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11406..11918
                     /locus_tag="KPK72_001570"
     CDS             11406..11918
                     /locus_tag="KPK72_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2488798.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11906..12433
                     /locus_tag="KPK72_001571"
     CDS             11906..12433
                     /locus_tag="KPK72_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2488799.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12504..13754)
                     /gene="clpX"
                     /locus_tag="KPK72_001572"
     CDS             complement(12504..13754)
                     /gene="clpX"
                     /locus_tag="KPK72_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG2488800.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13773..14357)
                     /gene="clpP"
                     /locus_tag="KPK72_001573"
     CDS             complement(13773..14357)
                     /gene="clpP"
                     /locus_tag="KPK72_001573"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2488801.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14507..15793)
                     /locus_tag="KPK72_001574"
     CDS             complement(14507..15793)
                     /locus_tag="KPK72_001574"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG2488802.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15987..16457)
                     /locus_tag="KPK72_001575"
     CDS             complement(15987..16457)
                     /locus_tag="KPK72_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2488803.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(16457..17806)
                     /gene="rph"
                     /locus_tag="KPK72_001576"
     CDS             complement(16457..17806)
                     /gene="rph"
                     /locus_tag="KPK72_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG2488804.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17966..19990)
                     /gene="ligA"
                     /locus_tag="KPK72_001577"
     CDS             complement(17966..19990)
                     /gene="ligA"
                     /locus_tag="KPK72_001577"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG2488805.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20176..21114)
                     /locus_tag="KPK72_001578"
     CDS             complement(20176..21114)
                     /locus_tag="KPK72_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG2488806.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21216..21638)
                     /locus_tag="KPK72_001579"
     CDS             complement(21216..21638)
                     /locus_tag="KPK72_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG2488807.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21826..23109
                     /locus_tag="KPK72_001580"
     CDS             21826..23109
                     /locus_tag="KPK72_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG2488808.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23187..23702)
                     /locus_tag="KPK72_001581"
     CDS             complement(23187..23702)
                     /locus_tag="KPK72_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2488809.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23702..25078)
                     /locus_tag="KPK72_001582"
     CDS             complement(23702..25078)
                     /locus_tag="KPK72_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2488810.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25103..25654)
                     /locus_tag="KPK72_001583"
     CDS             complement(25103..25654)
                     /locus_tag="KPK72_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2488811.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25712..25927)
                     /locus_tag="KPK72_001584"
     CDS             complement(25712..25927)
                     /locus_tag="KPK72_001584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488812.1"
                     /translation="MQIHRELLKSENEYMCTKKEKQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(25896..26729)
                     /gene="fdhD"
                     /locus_tag="KPK72_001585"
     CDS             complement(25896..26729)
                     /gene="fdhD"
                     /locus_tag="KPK72_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG2488813.1"
                     /translation="MNNIYSKTKLQENGKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26778..28922)
                     /locus_tag="KPK72_001586"
     CDS             complement(26778..28922)
                     /locus_tag="KPK72_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28506..28508),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBG2488814.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29173..30231)
                     /locus_tag="KPK72_001587"
     CDS             complement(29173..30231)
                     /locus_tag="KPK72_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2488815.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKYLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         9098 a         5447 c         3295 g        12420 t
ORIGIN      
        1 tttctacttt tactttatct tttaatctta atggatttac ttcaatagat ttatctgtat
       61 atttaaagta ttcttcaata aaacaatttt ctataccata gtaataaaca tacttaaaat
      121 cccttaataa tctttttatg aactcatatg atttttcctc ttttgatagc aagtatgttt
      181 ctcttatttt tttatctttt gttatattta taattaactg acttttttta cattctataa
      241 ataccccttc ttctgaaaaa ttctctatta aatttaagtt tataagcttt tgtaatatat
      301 caaaatttat atttatagtc tttggattca tatttagatt ttctgaaatg ttcttaatta
      361 attgaataat actctttggg aaaagaatta cttgtatatt caatttatca tttttagaat
      421 ttataacttt atattttatt gaatagttat ttatgtttat tggaatgtat tgtgttattt
      481 caaattcaat catgcttaat atgtctcttt ttttcttagt atttagaact tcaacatttc
      541 taatgactat atcctgaatt tgaatgttaa agtataaatt tttatataat atttttgatt
      601 taccaatctc ctcttttaca aatctaacta catcttctat atatttaatc ttctcaatcg
      661 aattactatc attttttata tcaaagttaa tctcttttat aattgattta tctataaaaa
      721 cttttttatg actttttctt aaaaatacaa gccttatata atcatcattt atttctaagt
      781 ttagaatttt tttaattata gccatctcct tcttaaataa aaataatttt aaaacttaca
      841 gcaatcaata taaaatatta ttttggataa aattgcttag aatttatatt tacgcttata
      901 tctgttccgc tatcatttat gacaagttcg aatttacctg atgtactctg aggaactgga
      961 acagtattta ggtatccctt ttccttcaat gtctctacac ttagagagtc tattgtcttt
     1021 tcatcatcat taattgccat gctagctgcg gttactatat tagcagcatt cgtatagtct
     1081 gcatttaatt tggcattttc ttgaacctta ctatacttaa ctaatgctat actagatata
     1141 acgcctaaaa tagttactac tacaatcatt tcaacaagtg taaatccttt tcgtttttta
     1201 tttatcaaat ttatcaatta aatattctct ccttttcaaa agtttcatct aataaaattt
     1261 atttttctct ctaaaaatta gtaattgcat caaacatagg aacaaccatt gctaaaataa
     1321 aagtaccaat aattaatccc ataaatacaa ttagcatagg ttctataaat ttcattattt
     1381 gctctatttt gttatttaat tcattttcat aaaatttatt tattacattt agacttttat
     1441 ctagtttccc actttcttct cctgtatttg ccattgaaat aaataatttt ggaaaaacat
     1501 ttgctaaatt caaagattta cctatgctat ttcctttttt tatataatta ttggaaatct
     1561 ttaacctttc ataaataaat tcgttgtcta taacactagc tgacatgtct atagcttcca
     1621 caatttgtat tccactgcta attaaaacgg acaatgccct tgaaaatctt gttgtagcta
     1681 ctaactgatt aatttttttt ataaaaggta ttttgaattt taacgaatta atcaattttt
     1741 ttacattatc atttgttata aagaaataaa tgcttcctat tgaaaacaat ataaaactaa
     1801 atattatata agctaagtta tttgtaacca ctgtagatat tcccattaaa actctagtta
     1861 ttaaaggtgg ttcaattcca ttgttggtaa aaaccacctg aaaattaggt attacaaata
     1921 cgaatataaa caacattgat aagatagata atattataag tattattgga tatattgaaa
     1981 tagttattat tttcattttt aatttgtatt ctttatcata ataattagat aaatcactca
     2041 ttattatatc taagtttcca ctagtttctc ctgcttttat catacttata aagaatttag
     2101 aaaatatacc tgttatttga aatgattctg ctattgagtt acctttttga atatgatttg
     2161 atactatact aagcaaattc tttacttttt tacttgactg actctcaatg atttcaaata
     2221 tttttgtaat ttcacatcca gattccagca atatgcctat ctctttgcaa attactctaa
     2281 tttctttatc actgattcta tttaatacta aataaaactt ttctaaatta taaaatatat
     2341 ttttagataa tctcatgtac tataatacct tccaaattat attaattaca attaaataaa
     2401 cttaatattt tctaatttaa tacatttaat tttatttcac ttaaatataa tttatactca
     2461 aacatcttaa ttaaaataga ttaaataaag acaactgacc aaacgaagtt taactataca
     2521 attcagttaa atcatttcat ttactactaa acactcttct aatgtcgtta ttttttcatt
     2581 tattagtctt ttacaactat cttcaaatgt tatcattcca ttattttgtg ccacatcttt
     2641 aatagtacta gaatcctcca tatttcttat acatgtcttt atatcatcat taatttctaa
     2701 gatttcataa attgctgttc ttccaaagta tcctttatca ttacattttt cacaccctac
     2761 agctacctta gtatttacat ctccactaaa attatctttt atcataatct catggctaca
     2821 atgattgcag acttttctaa ccaacttttg agatataaca cctataagag aggcatttaa
     2881 aagatatggt ggtatttgca tttctagtaa tctagctatg gaagatactg catcattagt
     2941 atgtaaagta cttataacta aatgacctgt tatagctgct cttactgcta tcttagctgt
     3001 ttctgcatct cttatttctc cgaccattat tatatctggg tcttgtctta atatcgctct
     3061 aagacctaca tcaaagctca accctacttt tgaatttact tgaatctgac ttatgccatc
     3121 catcttatac tctacaggat tttctatagt cataatattt ttatttattc cttttaaatc
     3181 atttaataag gaatatactg ttgtagtctt tccactacct gtaggacctg tcactaataa
     3241 tattcctgct cttttattta ttatacgatt tatagatttt atagcttcct ctgaaaaacc
     3301 aagttctaat ttgtctttta aaaaagtatc tctattaagt atcctcaaaa ctattttttc
     3361 accatagaca gttggtattg atgaaactct aatgtcaatt aaatttccac ctaactttat
     3421 atccacccta ccatcctgag gaagcctttt ttctgttata tccattgatg cctttaattt
     3481 tataaccgta gacaatgatg gatagacatc aactaaaaac tttgatactt cctttagttc
     3541 tccatctacc ctcattctca ctattaaatg ttctttaaat ggttctatat gtatatcact
     3601 tgcgttttct tttatagctt tttctaaaag attttcaaat aatttttttg cataatcctc
     3661 atcaagttca aagctattat aatatttttc aatcttttca cttataagtt cttttttctc
     3721 cttcttcaat ataatttctt tacctgtagc taatctcaag tcctgtagag cataaatatc
     3781 ctcatcttct atttctacaa ctaaattatc atcttgaaac tcaactggaa atacattata
     3841 tttttttgca agtttttctg gtattaacat tggcaaactg ctttcctcta atataatctt
     3901 aatcaccctt tatatacttt aatttattag gtatattcat ttttttcata gagatatttt
     3961 cttcagtttt tatgcttatt ttcattggat aattggtttg tatgctatct aaatatttac
     4021 tacctttatc tacataatta gatagtttct ccttatctcc aatgcacttt atttcatatg
     4081 gttgtgctac aggaactcca tttatattta tatggcttcc tgcaagtaca acttctgaat
     4141 attgattaat tctttgacca tttatttcta tgtattttcc tccgtttact tttaattcat
     4201 ttataacttt tacaagaatc ttattatagt ctatactatt agctatattc cctgtttttt
     4261 catctatagc atctatactt aatattatcc cactaccctt tacatctgaa tatgatagac
     4321 tatctctaag tgtttctatt tctgatattt cttcagaatt tttttgattt tgtttcattg
     4381 aattaatttc tttatctaac ttgtctttct ctttggttaa ttcttttata gattttcttg
     4441 tcatttttat ttcttttctt attgacttgt ctttgcttgt ataaactgtt tcttcattgc
     4501 tataatctct aaatatggtt ccaacaagta tacctaaaat agtacttgct agtattataa
     4561 acacatattg ttttttcata ctgtattgct ccttacaaat ttatttttat atgataattt
     4621 tctttaatta aatattaaac atttacatat gcttattata tcaaagttat atattaaaat
     4681 tcatctttag tatataatat catacattga actcctatca ctaatttacc aatatcaagt
     4741 atgcattttt agatgttctt aaaattatat aaactttcaa taaacattgt tgtgatatta
     4801 taaaaaaaca taaattagga aaggaaataa taattatgaa aaacaaataa tggctttgga
     4861 gattattaat atcatttttt agcaaaaaaa gtaaaatggc atagcctcaa agctacacca
     4921 atttactaaa aattatatta ttactaacgt taattttaaa catatgcgtt tataagtttt
     4981 cctcatattt taaaattctt ttaattaact tatctcttaa tttccctact tcatcaaatc
     5041 tcatattagg actatagtaa ctttctataa tatcatgttt agacttggct ctcttaagat
     5101 ttagtactgc ccaacaaatt aaatcatcta aaactttttc atcaaattta atatcttctt
     5161 catatttaag tagtttttct ctatttaaaa actgatttaa atcaattgta tcttcttctt
     5221 taaaagtcgt acttatagtt atgcctatat ctaaatcagt taatagtaca gtttcaattt
     5281 tctctggaat aagtggataa tgataaactt ctacatttaa acctttttca atagctttct
     5341 tatatacttt gtttaataat gtagttttcc cagttcctat ttctccatct aaataataga
     5401 ctttctttac atcttttaaa atgttttctg tataatctaa acaccctaca ggagttatag
     5461 ctgaaccaaa taaatgcttt tctcttttta gatttcctgt gtttttaact ccatcaaata
     5521 acctttttat aaattcttct gtgaccaaat ttacttttcc ataatccata caatcagaat
     5581 atttttcttc tatgtctcta aagattggtt ctgctgattt taaaaactta aatgctcttt
     5641 taaatgaatt actaatatct ttatcataaa ctgatatttc ttgtttattc ttttctaatt
     5701 cttctgtatt ccagtaatca cctaaattaa ttatctcatc caaagctcca ggtagctttg
     5761 ggtccatagt atgtggggca gttgcatcca taagtaccac gcctaatttc tttataacta
     5821 aaccatccaa cgaacttggg tcagaaggac aatgatataa atcaatatca tatcctctat
     5881 gcaaaaactc tttataaatt tttttcataa gtgaggattt tcctactcca ggtccaccct
     5941 ttaaacaaaa aactcttttt acattttcag gaattatata gtcaaaaaaa gaatatgagc
     6001 cttgtgatgt atttccaccc gggaatgcct tctttacctt tccttccata ttagcgcctc
     6061 ctcataattt acaatatctc ttaattaaga tattcatatt ataagaattt tattacagct
     6121 ttctgatttt gataaattta aaacttacca tctaatacta ataacaggtt aattttatct
     6181 aattattata gattctcata ctgttcctta ttctatctat aaatacaatt taagtgtcca
     6241 tattgaaata tttgtattgt aatacagctg gatattactt aaatccaatt gtttccatta
     6301 taattttatg ttaaaataat attacaaaat acatctgttt ttcttcataa acgggtgaaa
     6361 ttccctatcg gcggtaaaag cccgcgagcc ttatggcata atttggtcat attccaaagc
     6421 cgacagtaaa atctggatgg tagaagaaaa tagtatatga gtacctttat gtaattttac
     6481 atgagtaatc tatacaaatc cttcaactac cgtatttatt catgaaatta gacacattca
     6541 aggtacctaa tatacaggtg ctttttttgt tgtttatttt acaattatat cgtacttata
     6601 aaatctatta agattggagt gttatcatga aacaaaaatg gatagtattg attatcatct
     6661 gtattggtgt atttatgtct actcttgatg gaagtatact aaatatcgca aatcctacaa
     6721 ttgctgctga ctttaaaatt aacatgtcac aaattcagtg ggttgtaaca gcttatatgc
     6781 ttgttgtaac agctactatg ttatttttcg gaaaacttgg cgataaagta ggttcaaatc
     6841 gtctatacac attaggattt tttatattta caataggttc ttttctgtgt agtatgtcaa
     6901 acaatttatc aacattaatt tcttcaagaa tctttcaggc agttggagca agtattttaa
     6961 tggctactgg gcttggtata gtttccaatg catttccagc aaatgaaaaa ggaaaagcta
     7021 taggaatcac tggagctgta gttggtatag gaaatatgag tggtcctgta attggaggta
     7081 taattttaga acattttgga tggccttcta tttttattat caatatacct attggtatta
     7141 tagcagtctt tcttggcatt aaatttctgc ctaaacctgt tttggatgaa caaaacaaga
     7201 gctttgatat tccaggtctt ctacttttcg catcttgcac tacgcttata ttacttgcaa
     7261 tgaatgagaa aggtaataca agattgtatt taggtataac agcacttata atcttcttat
     7321 tgttggcatt aagagaagta aaatttgaac aaagctttat tgacctacct ctatttaaaa
     7381 atagaaactt tactgtaggc aatatcattg gagtagcttg ttattttcct caaatggcag
     7441 tatcttttct acttccattt tatttagaac aattaaaaaa tttatctcct atgatggctg
     7501 gttatgtaat gactgtacat ccacttatta tggtacttat agcacctatt gcaggttcac
     7561 tttctgataa acacggtgca aaaaacattc taacagcatc tttttcattt atgaccatct
     7621 cactagttgg aatggcactt ttaaaagcag attctccatt gtatctttta attgtatgtt
     7681 tagttatttt tggacttggg ctaggagcat ttagttcacc aaacaatagt agtattttag
     7741 cagatgttcc tcctcaaaaa cagggatacg gaggcagttt tcttgcaact attcgtaatt
     7801 tatcatttgc tcttggaaca gcatttttta gtagtttttt tgcacaaagt cttacataca
     7861 atcaaaagtt taaaagtcac acatcagcct atgttatagc tagcaatcag tcttattgga
     7921 ttgcagcatc agtatgtttt atagggctta tactaactgt tttctttatg agaaaaacag
     7981 ataaatctat ttcttaaatg ctatttattg ctgactacat aagtcaataa ttttttagtt
     8041 gaactaattt atactaattt tcccatacct tactaaaatt tattagtatt tagcaaaatt
     8101 actgatattt attgtttata aacaataagg atgccaaaaa aagttcatta aaactatttt
     8161 aaggcatcct atattttgct ttaatattaa tataattaaa actaactatt attacattta
     8221 ttattcaaac aaatcttatg tcaatattta acattttaaa tttaatctac tgttatttca
     8281 tatccatgtt gattgtattg atttacaatc tcttcccata attcttcaac accagtcttt
     8341 tttagtgaag aaacaggaat tactttttca ctactttcca tttgcaattt ttttcttatt
     8401 atacttatgt gtttttgata ttgtcctcta gatattttat ctgccttagt tgcaataact
     8461 acacagttat atccaaagtg ctttatccat tcatacataa gcttatcatc tgctgtaggc
     8521 tcatgtctaa tatctacaag taggaatata gaacataact cttgtctact ttctagatat
     8581 cgttccatta tacctcccca cttttctttt tcagacttag ctatctttgc atatccatag
     8641 cctggaaggt ctacaaaata aaattcatta tttattagat aaaaatttat agttctagtt
     8701 tttcctggtg tttgactggt tctagcaaag tttcttctgt taagcaatgt atttattatt
     8761 gatgattttc caacatttga cctaccagcc aatgctatct ctgggattcc ttctgctgga
     8821 tactgtgatt tattcacagc actcatagtt atctctgaac ttctaatttt cattattttc
     8881 accactcttt aataaagctt gttctaatac ttcatccata tgttctacaa gcacaaactc
     8941 cattttttct tttacatttt ctggtatttc atctaaatca gcttcacatt ctttaggaat
     9001 taatactttt gttataccag ctctatgagc tgctaataac ttttctttaa ctcctcctac
     9061 tgctaagact ctacctctta aagttatctc tcctgtcatt gctatatttc caggcacagg
     9121 tcttttagta agtgctgaga ttactgcaag agccatagtt ataccagctg atggtccatc
     9181 ttttggaaca gcaccttctg gaatgtgtat atgaatatca ttcgtcttat aaaattcaga
     9241 gtctatatca aacttgtcca ctattgacct tatataagag atacctgtct tcgcagattc
     9301 cttcataaca tctcctaatt ttccagttaa tacaatttcg cctttacctt taagtacgtt
     9361 tacttctact tcaagtgtaa ctccaccaac ttctgtccac gcaagaccat ttactaatcc
     9421 tatttgaggg ttaacttctg caagttgata cttaaacatg tcttttccaa gatatgtctc
     9481 taggtcagac ttatttataa caacctcttc aagagttggg tcttcaacgt attttttggc
     9541 aacctttcta caaatcttac caatagttct ttctagtgtt cttactccag cttctcttgt
     9601 ataatggttg attatacttc ttaaagtttc atcatctatt tttataaagc tttcttttaa
     9661 agcatgttct ttaatttgtt ttggaagtag gtattttttt gctatattta atttttcttc
     9721 ttctatatac ccagatactt ctattacttc cattctatct aataatggtc ttggtatatt
     9781 acctagacta tttgctgttg taacaaatag tatttttgat aaatcaaaag gtatttctaa
     9841 gtaatggtcc acaaaatcct tattttgctc tgggtctaat acttctagca ttgctgaagc
     9901 tgggtcacct ttataatctg ctgccatctt atctatctca tcaaataaga atacaggatt
     9961 ttttgtttgg gcttctttta ccccattaat tattcttcct ggaattgaac ctacataagt
    10021 tcttctatga cctcttattt ctgcttcatc tctaactcca cccaaagata ttcttacaaa
    10081 ttttctattt aatgctctgg cgattgattt tactatagat gtctttcctg ttcctggagg
    10141 accaactaga catattatag gacctttaag tgacttagca agagttctta tagctagata
    10201 ctctagtatt ctctccttaa ctttttctaa tccataatgg tcttcatcta atatatcttt
    10261 tgccttagtt atatctaatt tatcttttgt ctctttattc catggcaatg agaatatagt
    10321 atctaaatag tttctactta cagatacatc tggagacata gaagatattt ttgaaaattt
    10381 atctatctct ttttctattt tctctttagt tgtcttaggt gcttttattt ttttaagctt
    10441 ttctctatac tcatctgctt cagagtttat atcttcttct tcacctaatt ctttctgtat
    10501 agcttttaat tgttctctaa gataatattc tttttgaacc ttattcatct gtttttttac
    10561 tcttaaagtt attttctttt ctatttttaa tatatctatt tcctctaata atatagaata
    10621 aataagttca agtctttttc taatatcaaa ttcttctaat atttcttgtt tttggcttga
    10681 ttttagatat atgtttgcag ctatcgtatc tataaatcta tctacatctt ctatatcagc
    10741 taaagatatt aatatttctg gtgataccct atttccaata tttatatact cttcaaaggc
    10801 atcaaataca tttcttacaa atgcttcaat ttcaacttct gtttcactat ccaaattatc
    10861 actatcaaat actatttctt ctattactgc tctgaaatac ccatcttctt gttctatttt
    10921 ctttactcta ccccttgata caccttcaac caaaacacga actgtatctc ctggtaattt
    10981 tatcatttgc ttaactttac agattgttcc cacatgataa aaatcctctt ctcctggctc
    11041 atctacctct gcttctttct gagaagttaa aaatataagt tcttcatcca tcatagcttg
    11101 gtctaaagct ttaagagata tctctctacc tatatcaaaa tttaatatca tatatggaaa
    11161 tatagctaat cctcttagag gaattaatgg taattcatga tctattttag tataattttg
    11221 ttccatcaac attactcctt ttctagtcta attgctttgc ttttaatatt atatatttga
    11281 acaagttaat tttcaacatt aatgataaat tcttagaata ctatcatact atattatctt
    11341 tttattatga tataatatta atattagtat acttagatgt tatttatctt ttggaggttt
    11401 ctattatgtt gaaattgttt ttaacctttt taaaaattgg tgcgtttaca tttggtggtg
    11461 gatacgctat ggttcctctt atacaagatg aacttgtaaa caaacaaaaa cttttagagc
    11521 aggaagaatt tattgattat ttatccatag ctcaaagtta tcctggagta ctggcagtta
    11581 atatatctgt attattagga tataaacttt atggtatacc aggagttctt atatgtacct
    11641 taggctctgc tttaccatca ttttgtatag tcttagtctt atcgtatcta tactttaaaa
    11701 attcatcatc taaaatttta gatggatttt ttaaaggagt tgcgcctgtt gtaatagctc
    11761 ttatattgta ttcttttacg agcatgttca agaaactacc aaagtctaaa actaacatac
    11821 tactattaat tatagcagtt ttatctgttg gtgtattcaa tataagccct atttatttaa
    11881 taattggagg aggtgtatat agtctatgtc agaaataata ctactcttct taatattcct
    11941 taaaataggg gcatttagtt ttggtggcgg ttatgccatg ctaccattta tccagcaaga
    12001 atttatatct aaatatcact ttataactaa tcaagagttt ttggatttac ttgctatatc
    12061 acaatctaca ccaggtcctg tagcaataaa tagtgctaca tttgtaggat ttaagactgc
    12121 tggcttcttt ggttctctag ctgcaactat tggtgttaca atttttgcat taataggtct
    12181 aactattata tcaaagttat tttctaaatt taaaaataat aaaaaggtag aaaacttatt
    12241 gacagttctt agaccaatta ctctaggatt catattatca gcagcttttt catctgccaa
    12301 agaagttaat tgggacttat atgggataat tgcatttata gcatcatttt atcttttata
    12361 taaaggaaaa attggttcca ttgcaattgt attcgtcttt ggtattctag gtatattact
    12421 cacattttta taataatgat acctatttta tatttagcat attaacataa aaaaaggagc
    12481 ttttaaagct ccttttcatc atcttatgca ctttcctctt gatccttaag tactatgata
    12541 ggatttactg agtcttcgtt cacagatttc tctgttacta taaccttctt tatattatct
    12601 ctagatggta cttcaaacat agtttccatc attacgcttt caactatact tctaagtcct
    12661 ctagctcctg tatttctttc tatggccttt ttagcgattg cacgaagagc accttcttca
    12721 aattctagct caacatcatc taactccaat aactttttat actgttttac tagagcattt
    12781 ttaggttcct gtaatatctg cattaatgca tcttcatcca acaactctaa agtagctaaa
    12841 actggtattc ttcctataaa ctctggtatt ataccatatt ttaataaatc ctctggaagt
    12901 actttttcat atagcttccc taaatctaat tcttttttac tttctatttt agctccaaaa
    12961 cctagtgttt tatctccacc acgtttttga attatcttct ctaatccatc aaaagctcca
    13021 cctaatataa ataatacatt agtagtatct atctttaaaa attcttgatg aggatgtttt
    13081 cttcctcctt gtggtggaac atttgcaaca gtaccttcta atatttttag aagtgcttgt
    13141 tgaacacctt caccactaac atctctagtt atagatgggt tttcagattt tcttgcaatc
    13201 ttatctatct catctatata tattatacct ctttcagctt tttctatatc aaaatcagca
    13261 gcttgaataa gttttaaaag tatattttca acgtcttctc caacatatcc agcttcagtc
    13321 aatgatgtag catctgccat tgcaaaaggt acatttagag ttcttgctag agtttgagca
    13381 agtaatgtct ttccagaacc tgtaggtcct aataaaagta tattactctt ttgaatttct
    13441 atatccttac tactagattt tttactgtat attctcttat aatgattata aaccgcaact
    13501 gataaagctt ttttagcttt ttcttgacct attacataat catttagaat ctccatcatt
    13561 tcctttggtt ttggtaaaga tgtagtatcc tcatctatag tatcttctat ctcttcttga
    13621 ataatttcgt cacaaagttc aacacactcg tcacaaatat atacatttgg acctgcaatt
    13681 aatcttctta cttgatcttg atttttaccg cagaatgaac actttaattg tcgtttttct
    13741 tcatatttag acatattaac acctctctct atctacggtc tctttgtaaa tacttcatct
    13801 attaaaccat attctttggc ttctagtgca ctcatgaagt tatcacgttc agtatccatt
    13861 ttaatttttt ctaatggctg accagttctt tcagataata tctcatttaa agtttctttt
    13921 atttttaaaa ttctctttgc atgaatttct atatctgttg cttgaccttg agctccacct
    13981 aatggttgat gtatcattat ttcactattt ggtagtgcta gtctttttcc ttttgctcct
    14041 gctgccaaca agaatgctcc catagaagca gccataccta tacatatagt acttacatct
    14101 ggtttgatgt attgcatagt atcatatata gccattccag aagttatact tcctcctgga
    14161 gaatttatat ataaatgtat atctttgtct gggtcttcag cttctaaaaa taatagctgt
    14221 gctactataa gccctgctgt ggcatcgtta acttgatctc ctaagaagat tattctgtct
    14281 tttaatagtc tagaaaaaat atcataagat ctttctcctc ttcctgtttg ttcaacaact
    14341 acaggtacta atgccatggt ttcatacccc ctttattcta tataatttac agtagctttt
    14401 tttaagctac tgtaaattat tatacgcatt taactaaatt atatttgcac atcattatat
    14461 actaaacatt tcataacgtc aactatatta gttagcgttt attttattaa gctattgttg
    14521 cattatcaac taataaatct attgtttttc ttatttttat ttgacctttt atatcttctt
    14581 tgtcagcatc tcttaaagaa gcttctattt tttctacttc catattgtaa gcagaagcca
    14641 ttttttctaa ctcagcctta aattcttctt ctgttgcttc aattccttca gctttagtta
    14701 ttgcttctaa tactaaagaa gacttaacta attttttagc atcttctttt ctctcttctc
    14761 ttaattcttc catagttcta ccagtcatct ctagtaattg ttgtaatcca aatccttgat
    14821 attgtaattg gtagttaagt tcatttaaca tattatctat ttgatgttgt atcataactt
    14881 ctggtatttc tatttccgta ttttcagcaa ctttttctac tactgaattt ctagtttctg
    14941 catctgctct atttttagct tcttcttcta acttagctct tacatctgct tttaattcat
    15001 ctaaagaatc aaattcacta gtatctttag caaattcatc atcaagtgca gataattctt
    15061 ttacttttac atcatttatt tttacattaa atacaactgg ttttccagct aaatcttgtg
    15121 agtgatattc ttctgggaaa gtaacattaa cttctacttc ttcaccagct tttttaccta
    15181 ctaattgttc ttcaaatcct ggtataaatg tgtttgaacc tataactaag ttataatttt
    15241 cacctttacc accatcaaaa gctacaccat tttcaaaacc ttcgaagtct attatagctg
    15301 tatctccatc ttctaaagct ttgtcttcta cacttactaa tctggcattt ttttcaacca
    15361 tttcttgtag tttagcatca acattttcgt cacttacagt attctcaacc ttagctattt
    15421 ctataccctt gtagtttcct aattcaaact ctggtttaac ttctacatta agaaccataa
    15481 ccaacccatt gtctttactt atttcttcta tatctaaatc tggattgtct attgggtcta
    15541 tgtctaattc cttgatagct tctggataaa cttctgggaa taatatttct atagcatcat
    15601 tgtagaatat accttttccg tattgagtct ctataacaat tcttggagct ttaccttttc
    15661 taaatccagg tatattgtat ttatttctgc ttttattata agccttattt actgctgctt
    15721 caaatttatt gttatctact gttattttta aagtaacttt attaccctct tttttaatta
    15781 gttctgcttt catccttcta atcctcctag tgatatttat gtaatacccg ataacggtat
    15841 tataattcat tctatttata attcaaccac ttaattatat cacataagtt tttttattta
    15901 caagtgttcc attaatatcg ttgtacttat ttgataaaat ttaatttttt gagcgttttt
    15961 tgccattttt ttagataaat acacctttat atgcgtattt cttcaatttt tatgtcatct
    16021 tcttctatat ccataatgac aaaactttta tacgatactc ctcttggaag tgaagtgctt
    16081 ccaggattta tgtaaagtac accatcttta atttctctaa atggggtatg ggtatggcca
    16141 aatataacta tatcagcatc aacttcagtt gccttttttt ctaacatatt agttccatat
    16201 tttacaccat acttatctcc atggcataag aatatggttt tatttgccac ttcaaagaca
    16261 acttcttcct caacagcatc aaagtcacaa ttacctttaa ctgcaataat acctacatta
    16321 gtcatgctat gaatatatct tgaatcagta aagttatctc ctgcatgaat tattaggtca
    16381 cattctttca aatatggtat tgttttttcc atatttttaa tcatcatatg tgtgtcgctt
    16441 actatgccta ttttcattac ttcactaccc tttttataaa ctcttgtttc ataagtttta
    16501 gtgcgtttgc tctatgactg attgaatttt ttataacact aggtatttca ccaaaagtct
    16561 tatcatatcc ttttactata aataagctat catatccaaa accattttta cctttttctt
    16621 caaatcctat cattccttca caaattcctc ttactacaaa ttcttttcca tcaggaaaaa
    16681 ctactgctat tgcagagacg aatcttgcat ttcttttgct cattggtaca ttttgcatag
    16741 cttttaacag tttttttcta ttttcttcat ctgttgcatg ttctcctgca tatctagctg
    16801 aataaacacc aggttttttg cctaaagcat ctacttctaa gccagaatca tctgatattg
    16861 ctataagttt agtctttttt gctattgttc tagcttttat caacgcatta tgctcaaatg
    16921 tttttccatc ttctactatc tctatacctg ctaaatcaac atcttttaaa gagtacactt
    16981 tatattcaaa atcttttaag atttcaccta tttcttctag cttatgagca ttattagtag
    17041 caatcactac ttcattacca taatccatac ccaatatctc atctgatatt tcacctaaag
    17101 cttctctttg aagccttatt aattctttat tccctttttc acctaactct aacaataaat
    17161 ttaaatctgc tctcgaaaaa ggtttctctt ctcctgtccc ctgtacttct acaaactcac
    17221 acttatcagt cattattata ttcatatcta cctgtgcttt agaatcttct tcataacata
    17281 aatctagcat gtgtattcca tttacaatac caacactaat tgcagacaca aaatttgtta
    17341 ttggcatctc ttttattgtc tttgctttat ataatttata tattgcttct gcaactgcta
    17401 caaatgcccc tgttatagat gcagttctag ttccaccatc tgcctgaatc acatcacaat
    17461 ctacccaaat agttctttct cctaatttat tcaagtctac aacagacctt attgctcttc
    17521 ctattaacct ctgtatttct tgtgttcttc catcaatttt tcccctagat gcatctctta
    17581 tctttctttg ttgcgttgct cttggtagca tagaatattc agcatttatc catccagtac
    17641 cactatttct taaaaaagga ggaacttttt cttccactga tgcagtgcat ataacctttg
    17701 tctctcccat ttctataaga acagaacctt ctgcatactt tgtataatct ctagttatct
    17761 ttacaggtct tatctgttca ttacttctat cataacttct ggtcattttt tatatccttt
    17821 ctcatatttt ttatttttta taaatttatt tttataattt ttaattttgt caaatattaa
    17881 ttattgtcat ttttcgtaat cttattagct tactgaacta ttctatcatt aattatagta
    17941 gttttctact aaattaagcg actttttaac tctctaatac agcttttact tcatctttcg
    18001 atgataactt taataattct aaaaatctct cttcatctat aactttaatt cctaaatcat
    18061 ttgctttagt aagtttagaa ccagcttctt cacctgccaa aacaaatgtt gtcgatttac
    18121 taacactaga tgtagctttt ccacctcttt tttctatcat atcttttgca tcatttcttt
    18181 ttaatgtagg aagtgtacca gtcaatacga ttttcatccc ttcaaatatt ttttctactt
    18241 tttcacctgt attatccaat gattttgtat ttactcctac attttttaac ttgtttataa
    18301 cgttcatatt tttgtcttcc ttaaaaaact cgacaacgct gtttgccatt atacttccaa
    18361 attcctctaa ttcttctagt tgctcactag ttgcagaaat tacttcatct aaatccttaa
    18421 agttatatgc aagtatctta gctgctttaa ctcctataaa tttaatacct aatccattta
    18481 taagtctcca taaatcattt tcttttgatt tttcaattga atttattaaa ttgtcagcag
    18541 atttttcgcc cattctctct aggtcgacta catcttcctt ttttatataa tataaatcag
    18601 atacatcttg aataattttt tcatttaata gcagtgtaat tatagattct cccaatccat
    18661 ctatattcat tgcatccctt gacacaaagt gtataattcc ccttcttatt tgggctgggc
    18721 aagacatatt gatacatttt acagcagcct ctccttctaa tcttattgtt ggctctgaac
    18781 aaactggaca cttttctggc attttaaaat ctatttcatc tccagtacgt tcttctttta
    18841 taacttctaa aacttgaggt attatgtccc ctgctttttg aactaatact gtatcattta
    18901 tttttatatc tttttctctt atataatctt cattgtgaag tgtagctcta cttacagtag
    18961 tacctgcaag tctgactggt tcaagaattg ctgaaggtgt tattgtacca gttctcccaa
    19021 cttctactat tatatctact attttagttt tcttcttttc tgctgggaat ttatatgcaa
    19081 ttgcccatct aggacttttg gctgtatatc ccatttcgtc tctttgtttt atattgttta
    19141 cttttattac cataccatct atttcgtagt ctaaattacc cctgttatct gtccagtatt
    19201 caatatgctc aataacttca tctatacttt tacaaacttt ataactctga cttactgaaa
    19261 atcctagttt ttctaagtac tctagtgatt cactgtgact ttctaaatcg tattcttcta
    19321 tattttccat attaaataca aatatatcta atggtctctt tgctgttagt tttgggtcta
    19381 attgtctcaa tgaaccagct gcaagattcc ttggattagc aaataatggc tgttcttgtt
    19441 cttcttgttg ttcatttact tttctaaaat tatcttttga tatatatact tctcctctaa
    19501 ctattagttc ttttttatca tttatcttaa gaggtatgct ttttactgtc attaagtttt
    19561 gagaaatatc ttctcctaca actccatctc ctctagttgc tcctttttca aactctccat
    19621 tgttataagt tagacctaca gaaagaccat ctattttaaa ttcaacaaca tattcaacat
    19681 tttctcccac atattcttga actctcttat caaaatctct caaatcttgc tctgaaaaag
    19741 catttgataa actcaacata ggagttttat gtacaatttg attgaactta tctaatggct
    19801 tacctcctac cctatttgtt ggagaatcaa ttctctttag ttctggatgt tcttcttcta
    19861 aagaaattaa ctccttcatt aacttatcgt attcataatc tgatatttct gggctatctt
    19921 gattataata cctttcatta tggtagttta ttgtctctat aagtttatta atttcatctt
    19981 ttacgctcac tttttgcacc tcttcatatt tttaaaatta aattattttg aaatttccct
    20041 ataattaatt atctcctatt tatatatttt ttagtatatc acttttttgg ctttaaattt
    20101 attacaaaaa aaactcttat tagtatcttt aatacgacta ataagagttt ttatttttat
    20161 caaaattttt tatttttaat agcctcttga tatgtctaca acattatcta caggtttatt
    20221 ctccattata tagtgtttca aattattata aaccatatta aatgttctct ctttgttttt
    20281 atctgacacc caagaattat gaggagttac tatgacattt tcgcactccc acaacttatt
    20341 gtccttgttt aaaggttcac tttcaaatac gtctaatgct acccctctaa atttctctac
    20401 acattcttct aaatctttct gattaactgt atttcctcta cctacattta taaatacaga
    20461 accatctttc ataagtttaa acatatcttt gtttactatt ccatctgtac tttttgtaga
    20521 aggcattgct acaactacta tatcacattc ttcaaataca atattcatat cctcagtagc
    20581 aaaacactta tcaaagtatt ttttactact tccatcagta ttgacacccc atatttctac
    20641 accaaaagct tttaatcttt ttgctgcttc tgaagatata gtacctgttc ctaaaaatcc
    20701 aactctttgt ccatacaatt ctgatacaga aaaatccatc ttccattctt tattttgttg
    20761 ttgtttaaaa aattttgcac tatttttata tatttctaaa atatacaata caatactttc
    20821 accaataggt acactatatc cacctctatt attagctatc ttaatatttc tcttcaatac
    20881 ctcttctttt ggaagttggt ctatacctac actagttgtc tgtatatatt gtaaattttt
    20941 catcttagaa atatctaatc tctcaaatgg attgtatgta actagtacat ctgctgtatt
    21001 tacatcctca ttattttcaa tactatcttc agagtaatgt atgacatcat agcctaattc
    21061 tcttatttta tcaaattttt cttttccata atttattgta aataaaactt tcatctcaaa
    21121 tctcctccat ctacccacat ttaaaatttt atattctaaa atataatatg atactcaatg
    21181 tacattagtt aaaattacta taagatatct taaatttact tatgttcaat tgtttttcct
    21241 aaagacatag cattacgttc ttgaactaaa ttttctatta aattattcaa tgtccaatca
    21301 atagatgtct ctgttaccat agctgtaaaa ggtctattta ttctcacttt ttttagccct
    21361 tctaaaaatg catttacttg tgtgcttgga gtgttattaa atattacaga cttactctta
    21421 atcccatttt cacattcatt tgatatattt ccatctaaaa tgtctcttat tatattctct
    21481 ccatttttat cacttaagat gatatggtct cttattccag caaagttact tacgttttta
    21541 attaatgata attctttttt attaaagttt acaagcataa tacacttcct gttgtgtatt
    21601 ttatctaggt tattattatt tattttttca aatgacattt tatccatcct ttcatttatt
    21661 tatatacttt aatttacccc atacatttaa aataatcaat ttttatagtt aatttttata
    21721 ttaataccca aattatattt aaatagttag gctatccaaa actaatatat aaaattagaa
    21781 taattagatt aatatctaca aatcataacc taaaggagag ataccatgaa tacattacta
    21841 aacaataaat ttagcattaa aaacttttca aatgcttggg ttatagtact tggttgtatg
    21901 ttgatacaag ctgttccatt tagtattgta gcaagtatac agcctcaatt tataagttat
    21961 gttgtagaag gagaaggctt tagtttagct tctttttctc taatattcac tattggtact
    22021 attgcctcag caattgcatc accttttata ggtgtattat tttctaaagt caatattaag
    22081 atactatatt tattaggttg tataatttct agtattgctt ttgctacttt ttctatatgt
    22141 actgaactat ggcaatttta cactgtatca tgtattgttc aaattggcgc tacaattata
    22201 tctgctatag gaatacctgt tttaataagt gcttggtttg atgaagaaag caaaggaaaa
    22261 gctttgggct ttgcttttgc tggtggttct attggtagta tatttctaca accacttgca
    22321 gttcgcttat tagcaactca aggatacagt cgttcttatc taatatttgg aattatagca
    22381 ttaatagttg gtatacctgt aatattattt atactcagga tgcctaaaga tattaatgaa
    22441 aaggtaaaat ctaaaggtaa aaaagagaaa gcatctgata caatttctcc ttcagtatgg
    22501 ggttatacat taaaagaagc taaaaaaatt aaatattttt ggattatgac tattggattt
    22561 atatttgttg gtatatatgt ttcatcatta tccgtacaat atccaactta tcttagaaat
    22621 tatctaaatc ctattctaat aggaacagta ggctccacat ttgctttatt tgctctcatt
    22681 ggtaatttat ttggaggatt attgtttgat aaattcggtc aaacaaagcc acttattata
    22741 tcttttgtat tggttcttat agcaacttta tcattactat tggtagataa atcttctact
    22801 tttgcatttg tatttgccat tttgaaaggt ctttctgtat ttgcttatat gattggtcct
    22861 gcttatttaa ctggctcttt ctttggtcaa aaggagtttg gtagcatata tggaatcgta
    22921 caattaattt ttgcagtggg atttgcatta ggttcaacat tatttggatt gttagttgat
    22981 aattttggat acagattttc ttggtctatt ataattttat cagttataat tgcctatact
    23041 ttacttatta taacttctat aggaatgaaa aaattgaata aagaaagatt agataaacta
    23101 atgcaataat tgatttttag atatataaat attataataa aaaagatgga gcttttaatc
    23161 ataaataaaa ctccatcttt tttatattac aatatagcta attttctctt atttttaact
    23221 ttactatcta tatcctcatc cttaactata gtaaatgcct tagttggaca aacatttaca
    23281 catgcaggcc cttctggcga ttctatacaa aaatcacact tatttgctgt cattctttct
    23341 ctaaagcaaa gctttccttc atctacatct tttaatgtaa ctatgtctat tgcacctata
    23401 ggacatgcta acatacaagt cttacatcct atacaatttt cttcatttat ttttattgtg
    23461 ttaccaaccc taactatacc accatttgga catgcctttg cacaaggagc atcttcacaa
    23521 tgtctacact gtattggagc acttacttct tttgtcttta tgacagaaag ttttggatta
    23581 aaatttattt catctggatt ttggtaaaaa atatcttcat ctgaatgtgc aactacacat
    23641 cctatcatac aagttctaca tcctatgcat ttatcagggt ctgctataac aaaatagttc
    23701 atcacttagc cacttccttt cctatacaat atgtagtgtg taatagatga tgagattttt
    23761 ctcctaatgg ttctaccaga tattcatcat acagtttttt aatatcagga ttctcatgag
    23821 attttctaat tgctaaatgt tcatcatgca cgtatgttgc ttttgttcta ttttcataag
    23881 ccaattcctt atattcttct agtaaaattt ttggttgacc tccaccactt acacacccaa
    23941 caggacaagt cataacttca ataaaatcta catctaattt tcctttttcc aagtcttcaa
    24001 gcactggtat tacatttttt aatccactta ctactccaac tcttaagtct aagtctccta
    24061 ttttaatagt tgactttcta aaaccatttt ctcctcttac ttgttttact tctacatcag
    24121 gaatactttc tccagttata agctcatatc ctgttcttat agcagcttcc ataaccccac
    24181 cagtaacacc aaagatagtt ccagctccac tataactacc taaaggacta tcaaattttt
    24241 cttctcttaa atttttaaaa tcaattccca tgtcttttat taaataagct agttctctag
    24301 tagttaatac tacatctaca tctctatatc cactagaatc catttcttct ctatcacatt
    24361 catatttttt acatgtacat ggcattatag aaacactaaa tatctttgat gcatctacat
    24421 tatttatctc tgcaccataa gttttaaata ttgcaccacc catctgctga ggtgatttac
    24481 aagaagataa atgattagtt aatttaggat aatttttttc catgaatttt acccaagctg
    24541 gacaacaaga tgtaaacatt ggaagagttc caccttctgt cactctttta attaattcac
    24601 taccttcttc cattatagtt aaatctgctg caaaattagt atcgtagact ctatcaaaat
    24661 ttaaagctct aagtgctgct gccattttcc cagcacctaa gtatcctaaa taatatccaa
    24721 aatcttctcc taaagcaact ctaactgctg gtgcacattg gaccatagtg aatacatttg
    24781 ggtcacttaa agctttcttc accttatcta tatcacatac attgtatgct gcaaatatag
    24841 gttcatttat agactctggt atctctcttt cttgtttttt ctcttgtaaa aactcaaatc
    24901 cttcatctat aactgaggca taagatttac aagtttgaac acattgaccg cacataacac
    24961 atctatctaa atttatctct tgtggctttc cctcttcacc ttcaatagca tttactggac
    25021 agacctttga acattctcta catccagtac ataaatcctt atctatattt attatcataa
    25081 acgtcaccta cccctgtatc ctttatttat ttgttaacaa taaactaagt gcagcttgtc
    25141 tattcttagc ttttttgtca tctattggag taacaagttt taatgcttcc tgaggacatg
    25201 cacttataca agctattttt tcattgtctt tacataagtc acatttataa gctatcttct
    25261 tattttcatc taaaattact tgttctactt ctttaccatc ttcatattgt ggaagtaagt
    25321 ctatagctcc aaaagggcat gctagcagac aagttttaca tcctatacac ttttcttcat
    25381 ttacagacat aatattgttt tctttaacta tagctttttg tggacatgag tttaaacaag
    25441 gagcatcttc acaatgtcta cactgtattg gcatacagaa attgtcacct tttactaagt
    25501 aaagtctagg tataacaggt atatcaactg ttccaactgt ataacccaca ttattatgct
    25561 gattatgcat tgtaaaacaa gctacttcac aagctctaca acctgtacat ttgtttgggt
    25621 ctgctattac aaaagagcct attttatttt tcatattatc ccacctttca atttgtatat
    25681 ttccaaccct ctagttattt tcatcatatg tctacttact ttcatcatat atatttatat
    25741 atttcactat tttctcagta cttatatttc gtttacattt tttgcacaaa ttgtatgtgt
    25801 tattatacac cttatcagta tcatcaatta atactccaca gatttcacac tttgaatatc
    25861 tttgcttttc ttttttagta cacatatatt cattctcact ttttaataac tctctgtgga
    25921 tttgcataaa tattcatccg tcttcctctt acaaatccaa taagcgttat atttaatttt
    25981 ttagaaagct ctattgataa attagtcggt gctgattttg atactacaat aggaatttga
    26041 gtcatagctg cttttaatat catttccaat gatattcttc cacttacagc cacaatttta
    26101 tcttttaatg ttatgtcatt taaaacacaa aatccaatag ccttatccat tgcattatgc
    26161 cttgctacat cttcacaagt gactattttt ttatcaaaat taaatacaga tacgctatgt
    26221 accccaccag tatttttaaa taattctgat gaatttaaat ttctatccat tatactgtaa
    26281 atcgtatcat aatttatttt tatattgctc tttacaggag cacatgttat atagtctagt
    26341 gaatttaaaa atataatttg ctcatcttga tttttttgtt tctcttgaat agttactttt
    26401 gcaaaacgtt cactctcatt tatctcaaat tttaataagc tttttttgtt ttctatgtat
    26461 ccttttgttt tcaagaatcc tactataagt tcctctaaat tatatggggt acataaaaat
    26521 gtatttacat atttatcatt taaaataaaa ctaagtggat attctgcaat tatttcttca
    26581 ttatctattg atacttcatg ttcatttatt ttgcttactt ctacattaaa agaatttaaa
    26641 tatttgtagt ccactagact ataactattt tcaaaaactt tatccttacc attttcttgt
    26701 aatttagttt ttgagtatat attgttcata aaatattagc ctttctgcct tttataaatc
    26761 ttaaatctaa taaatcatta gatatttgta gctgtcattt tgtctttcaa actttgatac
    26821 tcttctctta cacatttttc tgctaattct tggtcttcaa gtttttcaag tttaacagca
    26881 caatatttat actctggtgt tttagatata gggtctaaat ttgctattgt aagttcattg
    26941 caagctccaa cccaccactg ataagtcata tatgttgcac cttccttaac tctatcagta
    27001 actgttgctc ttgttattac acttcctctt cttgaactaa ttctaactaa ttcatcatct
    27061 tcaattccta atttttcagc atcattagaa tttatttgaa cacgtcctgg ttcatcttct
    27121 aatgaactaa gtgttctaca attaccagtc attgttctta ctgaatagtg tccaacttct
    27181 cttacagtac ataaactaaa tgggtattca tcatcttcaa cttccatagg gggtctccat
    27241 tcagcagcga acaaattacc tttaccatta ggagtagaaa acttttgacc ttcatacaga
    27301 tacatagtcc ctttatcttc catactttca ctcttacaag gccattgaac gcatccttga
    27361 gtttctatct tttcataagt agcacctaag aaacttggac ataattgtct aagttcgtcc
    27421 catatttcct tagtattttt ataattcata ggatatccca ttgctgtaga aatttcgctt
    27481 ataatttgcc agtctggttt tacatctcct tgtggctcta tagcttttct catcaattgg
    27541 aatcctctat ctgcacatgt atatactccc tcatgttctc cccaagaagt agctggaagt
    27601 ataacatcag cgtgaagtgc tgtcttattc ataaatatat cttgaacaat gacaaattcc
    27661 aattcatcta atgcttctct aacttctgat gcatctgggt cactttgcac tgggtcttct
    27721 ccaaatatat aataagcttt aagctttttc tcttttagta ctaaatttgg aacttgagtt
    27781 aaactataac cattattagg agataatttt accccccaag ctttttcaaa cttttctctt
    27841 atcttatcat cagttacatt ttgatagcca ggatatacat taggaagagc tcccatatca
    27901 caagctcctt gaacattatt ttgtccacgt acaggtccta ttccaacgct ttctctacca
    27961 aaattacctg taagtagagc aattgatgca agtcccttaa ctacatcaac agcttgacca
    28021 aattgacaga cacccattcc ataaagtatc atagcttttt taccttttgc atattctctc
    28081 atagcttttc ttataagctc ttctgggatt cctgtaatct tttcagcata tttagctgta
    28141 tatggtttaa caatttcttt atactcatca aatccttgtg tatgattttg aacaaattct
    28201 ttattgtaaa gaccttcttc tattaataca tttccaaaag catttactaa aaccatgttt
    28261 gttccacctt ttataggtag atgtatatca gcaattcttg cagattcagt tactctaggg
    28321 tcagtaacaa ttaatttagc accatttttt ttagctttta ctattctatt tgccactatt
    28381 ggatgtgaat ctgctccatt atatccaaat ataaataaaa catctgcatt ttctatctct
    28441 ggtatagaat tagacattgc accactacct aatgagtaag ccagaccggc tacagatggt
    28501 gcatgtcaga cacgagcaca atggtcaaca ttattagttc ctattgtagc tctcataaat
    28561 ttttgcatta tatagtttgc ttcattacca ggtcctcttg cagaacctgt tcccataata
    28621 gaatcaggtc catatttttc tttaatttca tttagtcttg aagctgtata gtttatagcc
    28681 tcatcccatt caacctcttc tagtacacct tgttttctta tcattggctt ttttagccta
    28741 ggtgttagaa ttttagggtc atttagaaaa tcccatccat aacgaccttt taaacacaaa
    28801 ctttcttgat ttgttctacc attagccggt tcagctctta atatcttatt atcttttact
    28861 actagatata attggcatcc agctccacag tatggacaaa ctgttaaaat ttttttctcc
    28921 atacaattcc ccccttagat ttatcccctg ataagtaaaa tttatcttaa agataatgag
    28981 tgtttttata atatgcaaaa tcataaatat tttgcaatac tatctattaa attcaacaat
    29041 tttctcacaa ttcctcttaa atttcaaaaa aaatatgtca taataatctt tatatttcaa
    29101 ccatttaatt aaaatattct agttcttatg acatattttt taatatactc aatatatatt
    29161 ctttaaactt atttagtttt cacttaaaac aatatttctg ccaattctat ttttcattct
    29221 atagtgagta attacagtaa atattccact tccaatcaga atcaagtagt acataaatcc
    29281 accatataaa aatactgcat atcccatcaa aggttgaggg aatactgatt tgaatatagt
    29341 gaaaaaggct aattcattag ctcctacatt cccaggtgtt ggtattggag atattgccat
    29401 atataaaaat gcttgtaatg tcagtatata aatatagcta taaccttgta aattaaatgc
    29461 tttatatatc caaaatgaaa cactaaagta agcacttaat tgaataaaag ttactattat
    29521 agttgaaatt aatacctttt tattctttgc aaaaaaattt attgctttac tataatcatc
    29581 tataaaactt tctatggatt caatcttatt atctaaaaat tttaaaaact taatttttga
    29641 taaatacttt atagtaattc tcataaaata ttttattttt ttaggattta aaataactaa
    29701 tattatcata attaagacaa atgaattaat ggctatccct agaaacacta gaggcataat
    29761 cattttcatt tgtttttcta acatagcaaa attcattaaa acaagtattg tacaataaaa
    29821 agtaactact atttggaaga taatagactt attggtaaca actgcacttg ccttactaag
    29881 tggcatttta cattttttaa gtacatatat ctgtactggt tggcttcctg aagcaaaagg
    29941 agttattaaa ttataataaa atcccataat ggctagctta aaaccaataa atctaatatt
    30001 aactttgtga gtgctttcta taattatctt cattactact ccttcaagca ttatgtgaca
    30061 cattattgcc aaagcaccta tgaataaaaa cttcttatct accataacca taacatttga
    30121 tagcatttta atatccaatg ttttaaacac taggtaaatt gttataccta ttaacaaaaa
    30181 taaaaatgta tactgtagta aactctttct aatattcttt agtctatcca ttagaaataa
    30241 ccatcctttg ctcaagattt
//
LOCUS       DAEMHZ010000038        30070 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000038 DAEMHZ010000000
VERSION     DAEMHZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.038"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(50..1138)
                     /locus_tag="KPK72_001588"
     CDS             complement(50..1138)
                     /locus_tag="KPK72_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488816.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            1263..1991
                     /locus_tag="KPK72_001589"
     CDS             1263..1991
                     /locus_tag="KPK72_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG2488817.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(2180..2671)
                     /locus_tag="KPK72_001590"
     CDS             complement(2180..2671)
                     /locus_tag="KPK72_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488818.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(2682..3125)
                     /locus_tag="KPK72_001591"
     CDS             complement(2682..3125)
                     /locus_tag="KPK72_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488819.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(3115..3954)
                     /locus_tag="KPK72_001592"
     CDS             complement(3115..3954)
                     /locus_tag="KPK72_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488820.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(4173..5558)
                     /locus_tag="KPK72_001593"
     CDS             complement(4173..5558)
                     /locus_tag="KPK72_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2488821.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEDRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(5731..6474)
                     /locus_tag="KPK72_001594"
     CDS             complement(5731..6474)
                     /locus_tag="KPK72_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG2488822.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(6494..7756)
                     /locus_tag="KPK72_001595"
     CDS             complement(6494..7756)
                     /locus_tag="KPK72_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBG2488823.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQGIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(7940..8938)
                     /locus_tag="KPK72_001596"
     CDS             complement(7940..8938)
                     /locus_tag="KPK72_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG2488824.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(8922..10433)
                     /locus_tag="KPK72_001597"
     CDS             complement(8922..10433)
                     /locus_tag="KPK72_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2488825.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDISSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKDILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(10487..11449)
                     /locus_tag="KPK72_001598"
     CDS             complement(10487..11449)
                     /locus_tag="KPK72_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2488826.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(11497..12387)
                     /gene="rbsK"
                     /locus_tag="KPK72_001599"
     CDS             complement(11497..12387)
                     /gene="rbsK"
                     /locus_tag="KPK72_001599"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG2488827.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(12429..13442)
                     /locus_tag="KPK72_001600"
     CDS             complement(12429..13442)
                     /locus_tag="KPK72_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2488828.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(13671..14648)
                     /gene="bioB"
                     /locus_tag="KPK72_001601"
     CDS             complement(13671..14648)
                     /gene="bioB"
                     /locus_tag="KPK72_001601"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG2488829.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(14854..15117)
                     /locus_tag="KPK72_001602"
     CDS             complement(14854..15117)
                     /locus_tag="KPK72_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488830.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQTMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(15187..16260)
                     /locus_tag="KPK72_001603"
     CDS             complement(15187..16260)
                     /locus_tag="KPK72_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2488831.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(16519..17277)
                     /locus_tag="KPK72_001604"
     CDS             complement(16519..17277)
                     /locus_tag="KPK72_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488832.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(17274..18191)
                     /locus_tag="KPK72_001605"
     CDS             complement(17274..18191)
                     /locus_tag="KPK72_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488833.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDGGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(18191..18391)
                     /locus_tag="KPK72_001606"
     CDS             complement(18191..18391)
                     /locus_tag="KPK72_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG2488834.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(18396..19490)
                     /locus_tag="KPK72_001607"
     CDS             complement(18396..19490)
                     /locus_tag="KPK72_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488835.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(20000..20140)
                     /locus_tag="KPK72_001608"
     CDS             complement(20000..20140)
                     /locus_tag="KPK72_001608"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG2488836.1"
                     /translation="MNGEGCILSNKITKECYKIGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(20450..21757)
                     /locus_tag="KPK72_001609"
     CDS             complement(20450..21757)
                     /locus_tag="KPK72_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2488837.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIKKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(21822..22301)
                     /locus_tag="KPK72_001610"
     CDS             complement(21822..22301)
                     /locus_tag="KPK72_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG2488838.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(22348..23211)
                     /locus_tag="KPK72_001611"
     CDS             complement(22348..23211)
                     /locus_tag="KPK72_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2488839.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(23211..23966)
                     /locus_tag="KPK72_001612"
     CDS             complement(23211..23966)
                     /locus_tag="KPK72_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2488840.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(23993..24481)
                     /locus_tag="KPK72_001613"
     CDS             complement(23993..24481)
                     /locus_tag="KPK72_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2488841.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(24499..24900)
                     /locus_tag="KPK72_001614"
     CDS             complement(24499..24900)
                     /locus_tag="KPK72_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG2488842.1"
                     /translation="MNKYKVVILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(24903..25400)
                     /locus_tag="KPK72_001615"
     CDS             complement(24903..25400)
                     /locus_tag="KPK72_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2488843.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(25424..25828)
                     /locus_tag="KPK72_001616"
     CDS             complement(25424..25828)
                     /locus_tag="KPK72_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2488844.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(26058..28625)
                     /locus_tag="KPK72_001617"
     CDS             complement(26058..28625)
                     /locus_tag="KPK72_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2488845.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRISVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            28791..29534
                     /locus_tag="KPK72_001618"
     CDS             28791..29534
                     /locus_tag="KPK72_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2488846.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         9654 a         5552 c         3066 g        11798 t
ORIGIN      
        1 taagcgagat taaattcagt taatctcgct taaataatgt ctaaatttat tattccaaaa
       61 aatcatcaaa atctaatgaa cctgattctg caaaatcata taatgcattt tgctcaaaga
      121 aaaatttctc ttcaaattta atatagtcgc tataattaga ttgacttatt tcattgatat
      181 aattgcaatc attttcgtta taagtaccaa taaaaaactt atttcccatt aagtaatata
      241 tagctttcat atcattattt attatatata cattttcttt tacatggtca ttattaaaat
      301 taaatctagt aaagaatata ccttcattca tattactttt taacagaaat ggattttctc
      361 tataaaatac tcctgcaaat tcttcaggtg aatttacact gtaaacactt acaaattcac
      421 ttttagaaat ctcatcaaat acctcaaaat catacatagg ttctttatct gtcactaata
      481 gagaattaat tctaaaacca gtttcataac acttacttat gcaaaaagca attttacata
      541 tataatatcc atcattatct tcatataaag cttcacttat atatctgcct tgacctttat
      601 cacttaccac gtttttaagt aatgttccat ttatattagt aatgtgcata tttgcaatct
      661 cgtcacttcc agaaaagtac tttaaacttt ctctatccca tgctataaat ctcatggtca
      721 tatagtttat aaattcaact tcctcgtcaa ctcttttaca tattttattg tataattttt
      781 ctatagttaa atctgtttta tagtttttta taatatcaat atactcacat ttatctcctg
      841 gcaatagctt atcataataa agattcttat taccaaaata agatactaaa aataaagatt
      901 catccttcgt taattcaact ctatctgaac caaatcctcc catcaatctt tcttcttcca
      961 tataagcttc ttcttgtgtt gggttattta aactcacata atccgctaat cctaatccct
     1021 ctgcatctaa taaaaaatat tgacaaaagt gattgttttc atcatctatc caatttatta
     1081 taagacctaa actgcccata agtctagaat ttgtagcctt tgcaaaatta aaattcatat
     1141 agtcacatcc tttaaactct aaaatacttg ttttttaata tataaattat tatacatata
     1201 ttttaacaac ttagatacaa tttataatat aatataagga aatgattaat tgaggtggtc
     1261 ttatgaaaaa tatagcagct ttttttgata tagatggtac tctttataga gactctctta
     1321 tggttgaaca ttttaaaaaa ttaattaaat acgatataat tgaccaaaaa aattggtata
     1381 aacatgctag agacactttt atggattggg ataaaagaca aggtaattat gatgattatc
     1441 tattagaaat atgtgacctt tatgttgatt cactaaaagg attagataaa acctgtatca
     1501 actttacaag tgaccaagta attaaattaa aagcagatag agtttataaa tacacacgtt
     1561 caaggattga gtggcatcta aatcaaggac acatagttat atttatatct ggtagccctg
     1621 gatttcttgt tgaaaaaatg gcaaaaaaat ataatgtaac cgattttcta ggaagtgatt
     1681 atgtctttga aaataatata tttacaggca cagttattcc aatgtgggat tctataagta
     1741 agaataatgc tataaacgat tttgttgtaa agtatgattt agatttatca aactcgtatg
     1801 cctatggaga tactaatggt gatataaata tgctaaaaag agttggaaat ccaatagcaa
     1861 taaatccaac aaaggaactt ttatctcaaa tatcacttga tgaggaaatt tctaaaaatg
     1921 ttgagataat agtagaaaga aaagatatta tttattctct atctacagat gtaaatatag
     1981 tggatattta atttatacta tctattttaa aaacttatat tatatctaac atataaaagc
     2041 tttgactaaa aatcaaagct tttatatgtt gcactaagaa tatattaaat ttagctttat
     2101 gctttaatgt atattgagat atatcattta cacttatcca aactacaatt tcattaaaat
     2161 taaatataaa aatcaactat tattttttaa atagcatctt tcttaatcta ttattgtaat
     2221 aacctataaa agaactgtaa acatagtcat atactataaa ggctatctca aatgaaccta
     2281 taataaatat gtttattgga atataaacta ttgttcttaa taaaaaatac aatataagta
     2341 tcattatatt tgcaaatact aatttcataa aatactcaat atatatagac ctatcttttt
     2401 ctataagata ttttacaata ccatatattc caaaggtaag tgaatataca atccattgag
     2461 acttacttgc catcactata aatcctaaaa ctatagaacc tatataaaaa gcaattccac
     2521 tcttaaagcc ccattccatt attacaattg atactaaaag tgatgctaac cccataataa
     2581 acaatgtatt tacaggaata atatttgtaa gagtcaacaa tatcacattt aatgctaata
     2641 aaactcctcc ataagcaact tttatactca tatttatcat cctaacaaca agttattaaa
     2701 tctccaccaa aacattcaca acatgaatct aaacaccaaa gcttagcaca attttcacaa
     2761 caatcgtcac ctagacaaca acagcaatca tcacagcaac agcaacaaca tccatctaaa
     2821 tcattacgtc ttcttctatt gtaattgtca gccctattgc tataatcatt tctatattgt
     2881 gtataacttc taagtgcatc actatattca ctattttcag gctccataaa ttttgctctt
     2941 tttaagaagt cttcaccttc ttcataatat cctatgttca ttgcagaaaa gccagttaaa
     3001 taataccact ctgcacattt gtcagtcaaa gtttttaaaa aatcatatgc ttttttaaat
     3061 tcactatttt ctatgaatgc tcttgctctc ataaaatttt cattattata agtattactg
     3121 tacattcttt ccacctctac tttctaatat attaatatat cttaaataaa caccagaata
     3181 aattatattt tctattattc caacattttt atttagattt agattatcaa ttcccctagc
     3241 taaaaaacct aatgatgtta ctattatttt ttttactttt tcttttaact catcattttt
     3301 atcaatatac tctataaatg gattatatct tcctttctta aaatcttcat ctaaatcttc
     3361 ataggcatct agtagatata tatacttacc tatattaaaa cctatcattc ttaagtttct
     3421 ctcattttca tcttttttat agacaaatac ttcacccatt atatttccaa atatactaga
     3481 tacttcatct atatttatat ttttatcttt ttctaactta tttaattcat ttagttgttg
     3541 ttttatatac tctgcttttt caggatactt ttcatatgct aattttaatt tgtttttata
     3601 tatataatat gccaatttat cttttatgcg tttatcatcc attaaattat cttccaattt
     3661 ataatacgtt aaaagaatat tcatacttgc tgcatattct gtaatatcat ttgtaattac
     3721 ctttttcttt ttaaatggat ttactataca cacttcctct gtacttatag actctggatt
     3781 atatactgct gttaataaaa caattaaaaa tgttatatca taattcaaag aaaacctaga
     3841 gatttctcca tgatttcttt ttaagtattt acatagtcca caatagtaag ctttgtaata
     3901 atcatattct ctaaatgtta aatccatttt gtttatttta acatatccaa acataaatac
     3961 tcctttttgt actatttttt atttagatta tatatagtat aacacttttt atcaagatta
     4021 tataatttac agtattctaa gaaacttaaa aaattccttc taatcattgt atcttaaatt
     4081 taaaataatt agaaggttgg gttaaaaata atattaaatt atatacttat attaactcat
     4141 acaagttagt tatgcttata tcaatttaag ttttaaataa ttggtatata tttaaaacta
     4201 cctacagtat ccttcaattc tttttttgct ttgtttataa ccaattcatc aagcattaca
     4261 tcatttattg aacatgatat agatttagat gccaataaca ttgccttaaa tcccatatca
     4321 gaacctgcac tggcacaaga ttgccatgta tgggcagaaa taccaatagg ccaaactgaa
     4381 caagaacact gtgctgtagg aattatgtag ctaacatctc caatatctaa tgataatgat
     4441 atacagttat tatgagtaaa tgtatcatcc gtaacttcat catgcagatt catcttagta
     4501 atatccttat ttccacttaa tacactagaa acactacttc ttttatctat agacactgtc
     4561 tcagctaatt tatctgcaaa atagtagtca tcttcattat agttttgcac acctactaat
     4621 ttcatatttt gtgatagaat atctgttaat attgtatttg gaatatagtc atatactcca
     4681 cttataatgt catattccat aatagtacca gtcattaatg ctgctccttt tgctatatca
     4741 acaatcctac caaatacatc tcttacatgc tccattttaa ctcctcttat gtaataccac
     4801 acttctgcaa aatcaggaac tatatttggc atttttccac cattagtagt tacatagtgt
     4861 actctacaat tatcaaatat atgttctctc aaataattac aacctatatt cataagttct
     4921 actgcatcaa gtgcacttct tccgttataa ggagcttgtg ctgcatgagc actaatccca
     4981 ttatatctaa atttcacaga gtaattagca agtgttggga ttcttatcgg cgtatttata
     5041 tcaaatggat gccaactaaa agcacaatca attccatcaa aaaaaccttt ttttatcatc
     5101 aatacttttc cagataaatc ttcctctgct ggacatccat agtattttat agtccctttc
     5161 agctttttta gtttgattaa ttcctttact gcaattacag ctcctattga gcctgtacca
     5221 agtaagttat gtccacatcc atgacctgca ccaccatcta caataggttc tttagtagtg
     5281 gatacttttt gagacaaacc atctaaagca tcatattctc ccaaaactgc tataactggt
     5341 ctatcttctc cataactagc cacaaatgct gtatccatac cttctaatcc ctctatacta
     5401 aaaccatgtt tttttaaaca ttctttttgt aaatttgaag catagtattc tttatactga
     5461 agctctggat ttttccatat cttatctgat atatctctta attctccctg tatctcatct
     5521 atatattcca tcaaaaactt tttatttaaa ttatccatat taattatata aacctccctg
     5581 tttctaataa cttatcgatt atcagccaaa atgactaatt ttaacttatt taaaaatatt
     5641 ttctatcata caaccatttt atacaataaa acctcttgac ttgtaagttt cattttatca
     5701 agtaagaggt tttcaagttt tataaaattt ttagcatgtt aaccaatcaa gcatattaac
     5761 ccataattta ttatagtatt tccaatcagt aaattctttt ggaccccagt gaggtgcaca
     5821 gtcagaacta aagatagcag attttccttt tccaaactcg ccaacagcta caaatgggtc
     5881 tcctccaata gttgctaaaa caggacaatt atctctagct actgttttgt tatatcctaa
     5941 aaactttggc cattctgttg gtataccttt taatactgga tgttcagagt caataacttc
     6001 tggaattatt cctgctggtt tttcaactct atcatcttta tctatcattg ttattggtaa
     6061 tacatctttg atagcagttt ctccaaatct agcttttgca tctattcctg taaatgacat
     6121 atatccacct accatgatta acgccccacc attatttaca tactctttga ttagttcaag
     6181 tctatctgga ttactattgc aatctataaa agtactatta gataataaga acgtattaga
     6241 gcctatatca gataatatta tacagtcata ctcatttaac tcttctaatt tatacggaaa
     6301 actatctgct gctacatgtg caggcatatg aactgtctca tatcctccag attctattgc
     6361 ttctttcaac cactttacag cttcttcata atgagttgta acaaacgtat caaatccttt
     6421 tatatgagtt atatttttta cccaagattc ccctactatt aatacttttt tcatttatat
     6481 attcctccta cttttataag tttaatatta gacttgcata cataagtata ctatctaaat
     6541 actccttaat ttcaacatat tcatcaattg aatgacattg ttctattgaa cctggcccta
     6601 atataagtgt tggtatttca gctaaatttt tcattactct cgcatcattg ccagctgttg
     6661 aaccttttat ctctaatttt tttcctagag tttcttctag tattttatgt gcattcacta
     6721 caaaatcaga atccttgtcc atctcaaaac ctaaccctga ttgatatatg tttatatttg
     6781 gtttattatc tttaagccat aaatctccat tagcccttgt cattatgaca tttgttacat
     6841 catttacaac ttgttcataa ctcattatat ttggcaagaa gtgtacacat aaattaaata
     6901 cacacttatc tggcacagta gaacctgctg ttccaccatt tattacacct aaatttattg
     6961 taggtgaagg taatagtgga tgtttatata tcataagcca gttatgttcc aactctttta
     7021 taccttgaag caacaacata gccttctcaa ttgcatttac accctcccac ttactaccac
     7081 agtgaagaga cacaccttta acctcaactt caaagaatac aaatcccata tgtgcaacta
     7141 ttaagttttg ttctgatggt tcacaaatta tgcaacaatc tccaccaata ccattcataa
     7201 ctgcatttat ggtaccattt ccgccacctt cttcatctac tactgacatt atttttacat
     7261 taccaggtac atttagacca ctatctttta taagctttac agctaatatt gatgctataa
     7321 gtccagattt catatcagca gttccaagtc cataaagttt tccattatct tctgtagcac
     7381 gatatggatt atatttccac ttacttatat cacccggtgg catagtgtct acatgaccat
     7441 taaatacaat tgtcttacca ggtaaatcat cactatactt acaaattaaa ttatatctat
     7501 cttgataatt gtgacctaga tttccttctt tatatatatt ttttgctttt ttaattaatt
     7561 cttcgctcat ttcttgtcta tctacactaa agcctatgta atttgcaagt ttttcaatat
     7621 attcctgtcc ttctttttca aatccaccta gtattccatg accaacatct tcagtcttta
     7681 tacttacaag ttcttttaaa taatctatgt attcttgttt tttactattt aatgtatcta
     7741 agagaatttt tttcatattt cctcctaaga tttattttta catttaaaat ttcaagtcta
     7801 atctcatcta tttaagccat agtaaacttt ttcacttagc atattcccat tcaatgtata
     7861 aagatgtatg caaattaaag tataaaaata tatacaaaaa cttaataatt tttatagttt
     7921 ataagctaaa atttaattac tatttagatt tagctgattt ttttaatttt atattattta
     7981 tccatatagc aaataacatt accaaacctt ttataatcat ttgattatat gaagatacac
     8041 ttaataagtt taatccattt cctaatattc ctattattcc gacacctata catgtaccta
     8101 ctatagaacc agaaccaccc atcatattag tgccaccaag cacaacggct gctatagcat
     8161 ccaattctgc tccatctcca acagttggtg aacctgatgc caaacgtgat gatagtatta
     8221 tcccagctaa tgctgttagt agtccagcta gtgcgtatac ttttactata actttattta
     8281 cttttactcc acataatcta gcggcctctt catttccacc tactgcatgt acatgacgtc
     8341 caaaattagt tctttttaat agaaaatgtc ctgctattac aactataatc ataattataa
     8401 ctggtattgg tattggtcca atatatcctc taccgagcat cttaaatgca tctgtattta
     8461 ctgatatagg tcttcctcct gtaaatacaa agcttgcccc acttattatc gttaacatgg
     8521 ctaaagttac aatgaatgga ggtagttttt gtgttgaaat aattgcacct tttatgactc
     8581 ctaatattgt ccctattgct aatgcaagta tcatacctac ccatataggt gcattataat
     8641 tcactactgt tgtagcccaa aatgtaccta ctaaagcaat tactgctcct acacttaaat
     8701 ctattcctcc tgtaattatt acaaatgtca tacctactgc aagtattgca tttattgata
     8761 tttgtcttcc aacattaatt aaattatcta aagttaaaaa gttagatgaa gctacactca
     8821 ttattataaa aattattaca agtcctacag ctgcactccc ttggtctcta aatacacttg
     8881 ttaattttgt caataatgga gaagattttt cttctttatg cttagttatt gaactcatta
     8941 attttacctc cgaaagccaa ttttaataca ttattttcaa ccatttcttc accttctaaa
     9001 aatccactta tatgaccttc tctcataacc attattctgt cactcattga tattaattct
     9061 ggtatttcag aagatatcat tattatactt acaccactct ccactaaatc tcccattaga
     9121 ttgtatatct ctgccttagc atttacatct atacctctgg ttggttcatc cataattaat
     9181 atatctggtt gtaataaaag ccatcttgct attatagctt tttgttggtt tccaccactt
     9241 aaactttgta taacttgatt catatgtggt gtttttatct ttaaagcttc tatgtatttt
     9301 tctaaaatat ctttttcctt ttttctatct ataattgatt taaacttaga tatctttttt
     9361 aacccagcca tagttgtgtt ttctttgaca ctcatttgta aaaacaatcc agtacctttt
     9421 ctatcttctg ttattaatcc tagtccattt tctattgcat ctgatggagt tttaaacttt
     9481 acttcttcat tgtttaaata tattttccca gaatcatatg ttctaagtcc aaatatagct
     9541 tctactattt cacttctctt acttccaact agtccaccta ttcccaatat ttcacctttt
     9601 cttatattaa agctaatatc attaaaaaca ttgttcttag ttagattttc cactcgcata
     9661 atttcttctt ttatttctac ttttcttttt gggaaaaatt ggtctagctt tctatctatc
     9721 attaaattaa ctaattcttc ctcagacgat atatctttgg tattcaaagt cgaaatcgtt
     9781 ttcccatctc gcattatagt tattctgtca caaatctcaa agagttcttc catcctatgg
     9841 gatatatata ttatactaac tccactctct tttaaatcat ttataagtct aaaaagtatt
     9901 tcagtctctt ttttacttat tgaagaagta ggttcatcca ttattaatac tttagtttct
     9961 gatgataaaa ctcttgctat ttctaccatt tgtttttgag ctatactaag ctctttgact
    10021 aaactatttg gatttatatc tattcccata ttatcgaaaa tctttttagc ctcttcattc
    10081 atttttttgt cattggttaa gactttgcca tttatttctt ttcccataaa aatattttgt
    10141 gctactgtta attcgtcaaa aactgttagt tcttgatata tggcatttat acctatcttt
    10201 ttagagatat ccggggatat attctctatt tctttgcctt caaaaataaa ttttccagaa
    10261 gttttcgaat gtgcaccagt taaaatcttt atcattgttg atttaccagc accattttca
    10321 cccagtagtg catgaacttc gccatgaaat aactcaaaat taacattatc taatgctctg
    10381 acaccaggaa attctttagc aatattactt aacttcagaa ttatattact cataatgaca
    10441 ctccttttta aataggattt ctttcaaata atctcatttt attttactaa ttcttaaagt
    10501 cttttatatt ttccttatca taaagtaaag ctggtactgg tatattcttt tctacctttt
    10561 ccccttttat agcttttact actgcttcta cagctaaacg tcccatctct ttaggttgtt
    10621 gttgaatcat agcaagaata gttccatctg ttattccact tgatgcttct tcagttaagt
    10681 caaatcctac aatcttaaca tccttatttt ttgcagattc aagtgcagct ttagctccta
    10741 gtacactatt ttcagctgtt gcatatatta tatctacatc tggattagat tgaattaagt
    10801 tttctactgt attaagagat tcttctctgt catatcctgg ttgtgaattt aatatcttaa
    10861 catttgctga accttttaat gcatctttaa atccatctat tctctgcatt tgtatttgag
    10921 attttaaatc tgtcactatt gctatttctg actttccacc taacttttca tctatgtatt
    10981 ttttagtgta ttcccctaat tttttaccag catcataact aactgttcct acataacttg
    11041 taatattttc agtatttaat acagcatcta ctgtaactac tggcattttt tcaaatgctc
    11101 cttctactgc tggtactata gccgaattat cagttccaag tactatcatt ccatctacat
    11161 tctgttgtat aaaatcttct attgctgatg tttgcttagc tacatcttgg tctgggtcat
    11221 ttactataac tttcacacct aattctttag ctttttcttt tattccctca tcaatatcta
    11281 taaaaaattg atgttgaaga gttattgtag atactccaat agttaaatct ttcttgtctg
    11341 aaccaccttt attatctgat ttacttggtc cttccattga acaccctgtt aacattccta
    11401 ttgctagtat tccagcaatt cctaaagata aaatttttct cactctcata attaacctcc
    11461 atttttaata gttttaaatt tgaacaaatt agatttttat ttattattta aatatttttc
    11521 aacatcttct ttatagggca taccagattg tgcacctagt ttagttacag aaagacctga
    11581 tactactact gcatattcta ctgctgtata taaatttttc ccttctgtta tagccactgc
    11641 caatgctcca ttaaatgtat ctcctgctcc tgtggtatct tgaacatcaa cacttataca
    11701 agatacatgc ttgatttcct caccatcata aaatctagct ccattttttc cttctgttat
    11761 tacaagttta tttggatatt tttttaatac ttcttctatc ccttcatttg tgtcaaaaac
    11821 tatcttatat tcatgttcat ttggtgttaa gtaagttact ttttctatta tatcttcatt
    11881 tagttttact gctggtgctg gatttaataa aactctaatc ctattttcaa aacagaaatt
    11941 cactacataa tttattgttt ttaatggaat ctctaattgt agcaatacta tatctgcatt
    12001 ctttatctct tcttcattct ttttaattaa ttctatatct actagctcat tcgtccctgg
    12061 tacaacaaca atactattat cattttcaca taatgttata acagcaacac cagaactttt
    12121 gtgattattt ctggatacta agctataatc aactttattg gttctaaaat ttcttattaa
    12181 ctcctctcct aagccatctt cccctatgca tgatatcatt tttacatttg ctcctagttt
    12241 agaagctgag atagcttgat tagccccttt tcctccaggt gatgtaaaaa aactattacc
    12301 taatactgtt tctccttttt ctggtctttt atctacagaa cataccatgt ccatgtttac
    12361 acttcctatg acaactatgt ttcccattac attactcctt tacatatcta tttatgtctc
    12421 tcgtgctgtt attcactttc ttttgttgaa tctcttatga ccaattctgg tttgaaaatc
    12481 aattcatcaa acgattcttt tttatctcct cttaaacaat ttatcaatat ttcactggct
    12541 ttatatccta tatcataaga cggctgtttt actgtggtaa gagaagggct tattagactt
    12601 gatgtttgaa tatcatcaaa tcccacgaca cttatatcat ttggtatact taatcctctt
    12661 tctttaagac ctcttatgac acctattgca atcaaatcat ttgcacaaaa tattgcatca
    12721 aaatctatct tttctaaatt ttttataaat tcttgtcccc attcaatctt atattttcct
    12781 tcaaatacat aagcaggatt gtagcttaaa ttacactcga ttagagcctt tttatatcct
    12841 tctaatctat ctactgctat ttcattttga agtggaccac ttaggtatat tattttctta
    12901 tttccaatat ctaataaatg ttttgtagct atataggccc cttcaaagtt atttatttta
    12961 acaattcctt ttaaggtttc tttgttcata cttatcttct tatcaatcaa gacagttggt
    13021 atttttatat ctttataatt tgcttctttc cagtttgtat ttgaagatgc tgcaaatata
    13081 attccatcta tcatcctctc tgatagtgtt cttattcctt catattcttt tttagcatct
    13141 tcatctgtat tacataaaat tatattgtag ccttcttcat tagctttgtc ttctacacct
    13201 cttgatacat ctgtaaagaa tggatttcgt atatctggta taagaagccc tatagtatta
    13261 gtttttttag ttactaaact ttgtgctaat ttatttggta cataattttt ttccttcatt
    13321 attttcaaaa ttttttctct tgttgcctga cttatatttt catctttatt attaatgact
    13381 tttgacacag ttgttttaga aacaccagcc aattcgccaa tttcttttat agttatactc
    13441 aagaagttca cccccaaaat ttaggaaacc gttttactta accggttacc tagatactat
    13501 cacatctttt ttccatttgc aaccatttta tataaatttt gatatttttc ccttttttca
    13561 aaatattgca acttataatt caattttaat atttaaatta atctgcaaaa aaaaattaat
    13621 atacttttat aattatctaa aagtatatta attaatttaa gcagtattta ttaaagttca
    13681 aatccttgct ctataatcat ttttttatca ttttcaatcc cacttccaac agttgtcaaa
    13741 taatcaccaa caattgcaga atttactccc ccattaaatc caactttgtc atatcccttt
    13801 aatgatagcc ttccaccagc ataccttatg tacacattag gaattataaa cctaaatact
    13861 gctgttgttt ttagtatttc cattggttct aatacatcat aattttccat tggtgtattc
    13921 ttaataggat ttaaaatgtt tattggaaat gattttacat ttaattctcg tatttcaaaa
    13981 gccattttta gtctctgttc ttcactctca ttcattccaa ttataccgcc tacacataca
    14041 tctagcccag cttttttagc atcttttata gtttttatcc tatctttata tgtgtgtgtt
    14101 gtacatatat tatgaaaatt atctctacac gtctcaacat tgtggtgata tgtagtcaca
    14161 cctgctgact tcaacatttt agcttgctca taatctataa tacctaaaga tgcacataat
    14221 tttaaattag tattttccct caatccttca tagatattta aaatattatt aaactccttt
    14281 cctgacatac ctttaccact tgtaaccaat gagaatctat gcactccttt actctccatc
    14341 tctttagctc tatttaagat ttcattgtaa tctaataatg aatattcttc tacaccagtt
    14401 ttataataag aagactgagc acaaaattta cagtcttcac tacatttgcc agatttagca
    14461 ttcattatcg tacataaatc tgcttttctt cccataaaat attctcttat ttcatttgca
    14521 gattttaata gaatatcaaa atcgttttta ttatttgtat caaggcttat taaattaaga
    14581 gcttcttcat aacttatttc tttttctctc aatactttat tttttaattt tattatgtat
    14641 ttttccatat atcctcctat ttgtcaactt aatattttta tgtagtttac ttattatata
    14701 tagtattgca tatataacat ttattggtca actttatttt agacacaagt ttactataca
    14761 caaaaaaata ctgcttcaaa ataatattag tatatggatt atacatatta ttattaaagc
    14821 agtatttttt attagaatat aattcttact ggtttataaa actatccttg tcttaactcc
    14881 atccaagctt tccattgtaa taacctctaa gccttcatat tctctaaata gccattttaa
    14941 atccttcatg gtttgattaa cctgactttt tgttagatta tttataaaat attcttttct
    15001 agtttcctca tactctttgc tagtcaaaaa gtttcttaga aaattaaacg caaatacact
    15061 tggaatattg tttacattag ccttcttgct gttttccttc tcatagtaaa ttttcataaa
    15121 tgcatactcc tttgatactt ttagatttct cttacaataa ttatatgcat ttaagtgcta
    15181 ctattcttat aattttatta attcatattc tttacttccc atacccattt cacatgcata
    15241 gtctattatt ctagttccat ttacatgagg atgttcatgt ttgaaaacat catgacccat
    15301 ttccttacaa atcaaatcat agcatgcttg ttcaatagct acagggtctg ttgatgctaa
    15361 tattcctata tcatgggcaa ttggtctacc tgaccaaggt acacaatcac aaagtggagt
    15421 tacattcata acaaaagtta tataccctac cttgtctttt ttatttgata ctgctccata
    15481 agcgtattct gccatctttt cgacaaacac atctgagtca ctttcccatt gtatagttac
    15541 tgctctagtt ggacagacag ttggacattc tccacatcca taacatacat cagaatctat
    15601 aactgctttt ttatccacta tacttatagc ttttgtagga caagaattaa cacactttcc
    15661 acaacctaca cacttctttt cctttactac tggagtaaca tcagaatgtt gcatttgttt
    15721 tcctgctgca cttgcacatc ccatagctaa gttttttaat gctcctccaa aacctgctgc
    15781 ttcatgacct ttaaaatgac tcattactat cattgcagaa gaattataaa tttctccacc
    15841 tatttttaca ctttcaaaat gttttttgtc tatttttaca ttctcataat ttctactata
    15901 taatccatct gcaattatta ctggtgcatt tacaactgca taagcaaatc cattttctat
    15961 agcagttgta agatggtcta cagaatttgt tctactccct gtatacagtg tatttgtatc
    16021 tgttaaaaat ggctttcctt cgcagtcttt aaccttatca accacttgtc taactgccac
    16081 aggactcata tatgtagtat ttcccttttc tccaaagtga agtttaattg cgacttggtc
    16141 atttttttct attaaatctt tgaatcctgc ttcatcaaat aatcttctta cattgtttgg
    16201 tacgttttta ttttgcgaat ttgagtataa atcacaaaaa tatacttttg acttttccat
    16261 atctacaccc ctttaaaata tttacttata ctaattttat cacttttaaa gtaattttta
    16321 gtatatttta acataattaa atattcttat atttatttga tacaaataat attatttgta
    16381 ttattttcta cacttaattc tatatattaa actacaccta aaaatcaaaa agacttgaaa
    16441 cttaagtcca agtctttttg atttttaggt gtagttttta acaacataag attactttac
    16501 cttgtgataa aggtattatt aaactggttt tttattaaat aaaataacag ctgctagtaa
    16561 acatactata gaaataagaa ctgttataat aattggaata ataaattctg gcattgccac
    16621 tttccctgaa atcaagtcta tattttttga catcaagaaa ttaggagtat aaggtgcaat
    16681 ctgtttagga atagaaatta aactcaataa gagagttgct accaatacaa gtaaaacact
    16741 ggcaaatgct ggtctaaata gtacacaccc aagaattaat acactaatat acataattcc
    16801 tataagccat aaattaaatg ctgaaaatat aatatgatta agatttgcag ttggccaaaa
    16861 atacattgta tagccataag caaataaaaa actcaaccaa aaaccaattg tcataataat
    16921 caccgtaaca gagaatttag acaatactat agatgaacgt gataaacctt tagtcagcat
    16981 aataacaagt gtccctttag aatattcatt tgataaaata ttacaaaaaa taattagagt
    17041 taaactcata ccaagaccag agatattgct aaaaaattgt gtccaagaat ctaaagcagt
    17101 tggtgcagca gtaactttta aagtcggtgc aaaatgagca atcaagtctg gcataaattt
    17161 tgcagaaata ggactactta taccaaatat tagaaataaa ataattaaac taaataattt
    17221 ataatttctt aaattctctt taaattcttt ttttgtaaat gctatgtatt ctctcacttt
    17281 actacctcca aaaataaatt ttcaagtgat aattctatta gttccatctt tactggacaa
    17341 agattcattt ctgcgagtgt agatataact gttttttgta ttgcttttat atctttacca
    17401 tgcaaaacaa tttccatatt agtttcttct gaatctttta gtaaggattt aataccatca
    17461 ctagacttaa acttttgaat ttcatcatta cttgcaaact ccagtaaaag tctatccttt
    17521 ccgtgcatac tttttatttc tgaaagtgta ccactaaggg caatgcttcc tttgtttaat
    17581 atagcaacat ggtcacagat tctttcaaca tctgatagta tatgtgttga aaatattaca
    17641 gttgttgagt ctttgatttt aagcataata tctaatattt cttttcgacc aacagggtca
    17701 agtgcacttg ttggttcatc acaaataagt aatttggggc gtgaaagcaa agcttgagct
    17761 ataccaagtc gttgcttcat tccacgagaa aacccaccta ttcgtctctt ttcatttcca
    17821 agacccacaa gtgataacaa ctcttcactc cttatctgta tttctttttt tgaaagtccc
    17881 gtaatttcac cacaaagaga aagatattca agtggtctca tatagttgta aaactctgga
    17941 acatcaggca gataacctac atgacgatta gaacttgttt tcccataggt cactttctca
    18001 ccacaaacat ctatagttcc accatctggt ttaagtaacc caagtaccat tttcattgtt
    18061 gtagtcttac ctgcaccatt cttacctaca aacccaaaga ctgacccttc tggtacagta
    18121 aaatttaaat tatctattat ctttcgatta ccaaaacctt ttgataatcc atttattgag
    18181 acaatattca tcattcttca cctcttccta ttgtaaaata caaaattggt cctattagac
    18241 taataaacag tactacaata atccatattg ccttatttcc aattttataa tttggatgtc
    18301 tcaatacatg aacaagtgca gtaattataa gtattaaatc taaaataata acaggaacca
    18361 aaagtggtaa gtattccatc aaattttcca taatcttatt cctccaataa agataatttt
    18421 tctataagaa atttgtattc tttgttttca cttaatacag aaaacattgg attattttta
    18481 atagcagcaa caatacctgc ttttacgatt gtatcatcac gtgtaatacc tgttccaatt
    18541 tcagaaatcc atctgtcaag tttattaaaa aagtcatccc catgtattat aattgggtat
    18601 atatcacgtg ttgctaaatc tacatatttt cgtaatgatt taatagctcc ttcattatca
    18661 ccatgtatta aattaatttg tgcctgtgtt aaatatgaag aaagcataat agcaggagat
    18721 aatgtatcaa gctcaaatat atctgaaatt gctagaatac gtctttctat ttccttcatt
    18781 tgtaatttat ctgcattttg caaaataccc actagatttt ggactaatga tataaggttc
    18841 tgatatgcac caagttgaaa tgtttctctt gcttcatcca tttgaccatt catcatttgc
    18901 ccttgtgcaa gaagtataga ttcatttatc atgggaaaat tgctgttttg caataaatca
    18961 atcacttgta tgggttgatt taacaatata taacaagttg cctccatact ttttgcttga
    19021 cggcaaatat ctatgtcatt acaagtttcc tgtatacgaa taaatatttc taatgcttca
    19081 ctaattaata gttcacgctt tttttcatct acaatatcat agtgattaac tattaaaaga
    19141 cccatccgaa aaatcaaagg aaaacatgaa taatattttt ttatgatttc tcttatttca
    19201 tccatgactt catcaaatgg tttaaccgta aagtctttgc acagtttatt atacagttta
    19261 ttaatatctt ccttcatcat ttgaggttca taacatatta actcatcaac ggtaatgttg
    19321 aaatatgtcg cgagttgcgg taaaaaggtt atgtcaggat agctttgtcc agtttcccat
    19381 ttagatacag aagctttaga aacgcctata taatttgcaa gttcatcttg tgtaattcct
    19441 ttttctttac gctttaaaat cagcgttttt gcgatgttta attcattcat gtcaatccct
    19501 ccttatgtat aattatatcc ttaaacatgt aaagctaaca agcgatttga agttgacttt
    19561 tgacgaaaaa atcaaccaca gagaaacaaa taaacaagta tataaaatat atatttaaat
    19621 ttatctttaa tctcttttgt aaaatatgta aactttgtaa ataaaatcca tgttatataa
    19681 aactaatagt taatctatct aaatacagtt tttatttatc acgtttttaa atattttgtt
    19741 taaaacctct tttaatataa acaccttatt ttcatttctc atttcatatt ctttttaaaa
    19801 tactgaatat aactcttcga ttttccattt aattctttta taaagttatt attcgtattt
    19861 attcctctaa ttataacagg caactatcat gtattggtat ttgtttatta tataaaaatc
    19921 tataaaagaa aagcagtcaa tcatttagat taactgcttg tacatatatc ttattaaact
    19981 gtataaacta taatttttat tattctccat tttcatccca agtcataaat ccccatccac
    20041 tatctggata agtatcaata gacttttctc taatcatata accaatttta taacactctt
    20101 ttgttatttt attagatagt atacatcctt ctccatttat ctaattccat ttaattaact
    20161 tttcttttca accttaactt ttataaaatc atctttttta ataatttcat acttattttc
    20221 tcttcaacat catcaaatga gggtgtctca aaattaactt tttacttcat aaggcactct
    20281 tttttgtatg aagtaaaata tattttcatc atttcaacaa taagtaagag gcttatctct
    20341 attttgagac agccccattt gatggtgttg aacatttaaa taaggttgaa ctcgttttat
    20401 ctctcttttt aagatatccg taaatttgca cataaagtct cttcttcttt tacaattcta
    20461 atagttttat taaagccaaa gtatatatct ccatattctt tcgtatatga tttattggta
    20521 ctctttcatc tactccatga ccaccagttt tatacgctaa gaacttaggt ccaaacgcca
    20581 ccatatcttt aaaccaactt gcatatgtgc caccacccat agcaattgta tatccatttt
    20641 catctgttac ttcttcatag gcttctaata attttttaag tactaaatta ctctcatcca
    20701 cataatgtaa gtctttatga gcaataacct ctattttcca accattacaa aaaacctttt
    20761 tgaatctatc tattatattg tttaaaacta tatttttacc ataccttata tctaactcac
    20821 atacgatttt ttcctcattt acttttaata ttcctaaatt taaggtagtg tttttgaata
    20881 cctcatcata ttgattaatc cccaatgcat ctccataata atcactacaa agatattttg
    20941 aaactaattc tatatcttct ctatgctgaa aatccatatt atcttcaact atctttatta
    21001 ccattttaga tatagcattt tctccctttt caggattact tgcatgtaca gctactcctt
    21061 tagtagttaa ctctgaaata attccattaa taatttttgc agtacaatga gatggaacaa
    21121 cattttcttt agttcctccc cttactatta tataaggttt acttttgtca attttaattt
    21181 ctttttttat ccttatatga acccttcctt tttctccatt aaccactgga tacatagcat
    21241 ctggagtaaa tcccatactt ggaatttctt cacctttatc taagtaatat ttgatacatt
    21301 tcatacctgt ttcctcattt gtaccaaaaa ttaatcttat tcttttatta attgtaggat
    21361 acatatcctc tatgtttttc aatacatgta taagcataga tacagcaccc ttattatcta
    21421 tgacacctct tccataaata atattgtcaa ttactgctcc cgaaaatggg tctacactcc
    21481 acccctctcc tggtggaact atatctaaat gcattggaat ccctattaat tcctctccct
    21541 ctccaatttc tgcatatcca atataattat caatattttt aacactaaaa cctaaactag
    21601 cacagtaatc taaagtttta tctaaagatt ttttaggtcc tataccaaaa ggacattgtg
    21661 aaataggttc accttgttgt gaatcaattt ttatcaattc ttttagaaat tctatggttc
    21721 tatttaaact attatctaca cttctgctta tatccataaa tcaacctact ttctatcttt
    21781 ttaaagcctg tccttaaaga acaggctttt agcattaaaa attatttttt tgtaaatact
    21841 aattcacaaa cttcatcatc atgagcacat ttttgagcaa aatctaattt aacaccttgt
    21901 gcacagtcaa ctcttccata atccccataa caagctaatc tacaaatttc ttttctttct
    21961 tctttagtta atccatattc tttccaagca gcatctaatg gacagtgatg gaattttaaa
    22021 acgccattct catctgtatt ttctactaat tccatttcaa acactttttg tcctttactg
    22081 ttatataaca tttctgccat ttttcctgga gtatctgcta cgttatattt ttgacctctc
    22141 attttaccaa atccatatat tgcttcttta catatatcat ctggattagc tcctaatttt
    22201 tcagcttctt tcataagaag atagaaccat actgctcttt ctcctgttgc tcctctaatt
    22261 ccttctaaat attccttttc tgtatacatt ttttctgaca tttctttctc ctcctaaaat
    22321 taaattctat attttaatca ctgtgaatta gaatattcca atgaatgacc ctaccaatgc
    22381 taatacaact aaaattacta ataacttagt tattgtccac ttcttgtatt tcattcccca
    22441 atatacgaag aatacaacac caagtggtaa taaacctgga gcaatcatat ttaatgtatc
    22501 ttgaacaaca attgctttac caccaatatc aaatgatagc ggagttgaaa gcttaactgt
    22561 agttgcagat aatgctccca tcataaacat tcctagaatt gaagcaccat caattatctt
    22621 attgacaata ccacctgata gaattttctt aattgaatct ctacctaaag aatatcctaa
    22681 atgaaataag taataacctt cgcaaaatag taatatacta aacattattg ggaaaaatgc
    22741 acctataaca cttccttctg ctccaaatga agcacctaaa gctaagaata ttgtttgaaa
    22801 tgtaccaaaa tctaaagtat ctccaattcc agccaaaggt cccataagac cattcttaac
    22861 acctgaaata acttctcctg ggatttttcc ttcagtagct ttctcttctt ccatagctaa
    22921 aactgtaccg tgaattaatc ctccccatat agcttgagta ttaaaaaatt gtaagtgtct
    22981 ctttaacgca tctactaaat cttcttttct cttatataac ttttctaaag ccggagtgaa
    23041 acttgcacaa actgctaatg cttgcattct ttcaaaagag ttggataatt ctgctgtcca
    23101 ccaccatctt agatatgttt ttgttacatc tttctttgta agtactcttg tcttattaac
    23161 ttcagttgga ccttgtccat ttttaacttc tttatttttt atttcttgca ttatacttca
    23221 cctacccttt tgtcttcttt cataaaggta attaaaagag ctatacaaac accaaatatt
    23281 gcagctgcag taattgatat ttcaaaatat tgaaccataa aaaatcctat aataaacatt
    23341 ggtaagaatt tatttttacc tatagtaaat attgttgttg caaaacctaa tgcaggtaat
    23401 ataccgccca ttactgttaa tccattcatt aaccattgtg gtataacatc taataatgat
    23461 tgaactaaat ctgctccaaa aaagttacat acaaaaacta ttggaaacct taaaacaaac
    23521 ccaaatatta aagaataaag tgttgcacat ctccagatgc cttttgtatt accttcttca
    23581 gcatacttat ctgccttatg aaccaataca gcattaccag ttcttcttaa gttattaact
    23641 aatactccta taacacctaa aggtaccgca atagatactg caacttctgc attaacacca
    23701 gtttgaagtg ctactggtat tgcaataaga gcagctagac atttatctga tggaatattt
    23761 ccccctgctg cgaccattcc tagataaacc atttctatac ttgcaccaac aatcatacca
    23821 gttgttacat caccccaaag taatccaagg ctaaaaccta taaataaagg agactttagt
    23881 gttgagaaaa gggtataccc tgcaagacct gctgcaaacc aataccataa tcctgttaca
    23941 attgccaaca tcaaaacaga ttccatttat aattcctcct aatttatatt ttttatttaa
    24001 atttctttaa cgcattttca tatgtcatag atgattcctc tggtataact tgtaggacta
    24061 tttcaacccc attttcatta agttcattaa gttgttctac atcctcattt cctaatgaaa
    24121 cagctgtaaa caccattttc tttcctgctt catttggaat acctcctaat tgaatctttt
    24181 catatttaag tccagcttta taagccttat ttgccatatc aacattttta aatagaataa
    24241 atatattttt atctcctaaa gtgttgtttt ggaatgcttg tcccgcatct tctgctgtta
    24301 aaataactac ttctactcct gatggtgctg ccattttata tatgtcagcc ataaattcat
    24361 cttgacctaa gaagtcatca actattatta ttttttgagc ttgagcgatt tttacccatt
    24421 ttgttataac ttgcccatga attaatctgt ggtctactct tactaatgtt atttttgcca
    24481 ttatattcac ctcataattt atattttttt aaattcttta attaaatcca cacaaaggtt
    24541 attatttttt ttgacgattc tttctggtaa tgtatgtaat ttatcttctt tgcttaattc
    24601 ttcatctacg attaaaattg tctgtaagct aagtccattt attgcatcta ttcctcttct
    24661 tatagcaaca gctattgcaa cacttgatgt agatcctcca tataaatctg atattataat
    24721 tttcacatct tctttatcta ctacttcttc aacctctttc ataaatacag aaacatcttt
    24781 ttcagaactt agtgataaaa cttcatattg atttgacaat ccaaagtttt tatttgtttc
    24841 ttctactaag acttttcccc aagttccatg gcacagtatg actaccttat atttattcat
    24901 tgctaaataa actctccttt atagcgatta atattttggt ttaattctac ttcttcttca
    24961 tcaggtacca tttgaattga tacattaata ttctttttaa taaattcatc tattaaaaga
    25021 aactcttttt tattaatgta tacattttga gatattttct tttttcctat tcctgtaggt
    25081 attcttccaa caataagttt tttgatatta atcccttctt ctatcattct atttgcagta
    25141 tctaaatttt taattaatac aaaaacgtta tcatcagctt gattattata aaaatatctc
    25201 cttgattcac ttacagttaa tatcgataaa tcaatattct ttggtgctgc catttttatc
    25261 aaacctttag caataacatc attggttgtt tcatcatcta ctactactat cttttttaca
    25321 cctagatgct taacccattt atatataact tgaccatgta ttaatctatc atcacatctg
    25381 caatatttta ctaaagacat ttttaatcaa actccttttt ctattacatt gagctaagaa
    25441 ctttattaat attgtttata ccagtttgtc ctgcttcaat acatgcttct gctaactctt
    25501 ctaaactttt tccctctcta ttaattaaag cttcaagtag cattggaaga tttactccag
    25561 taagtgcatg aaacttgcca ccttttttaa ggtttaaact tgcaacatta cttggcgaac
    25621 ctcctaatag gtcagctaat actaaaactc cgtcaccttc atctaattca tttaactttt
    25681 ctacaaaatt tttatttaag tcttcaatat tatcattcct atttagttta attgctgctg
    25741 ttctttctgc tggtcctgca ataagttcac agctttttat aatttcttca ctaaaactcc
    25801 catgagtcat aactattatt cctatcacca atttcacctc acttttcttg tcacttttta
    25861 tataagcaat tgccgtgcca acttatgtgt atcaataaca aatggctatt ttacaggatt
    25921 taatcaaaac tttttactca atttttttta aaaacttatt atttcgatat gtatcaaaag
    25981 attttttcaa cctgtatcaa atctgtatca aaaaaagtaa aaaaaagata gccttttggc
    26041 tatttttaaa ttttgtatca acttttgaat ttatctgtat catattgtga ttcaaatata
    26101 tctaatatat ttgcatattc catatctgaa ataattatat cgaatgtctc ttctactagc
    26161 ttaaaacttt cttttataac taaatataat tctttatttc tactaattct ttcttcatat
    26221 gatttataaa ctaaagactc ttttattatt actctttcaa tcatacatga acaatgaaat
    26281 ataaacttta ctaaaagttc atcttgaatt actaaagaat agtgttcact aattctatta
    26341 attacttctt ttaatataaa ataagccttt tccccattta aaaaagtcaa agttttacta
    26401 atattatcaa ttagtaatct atcaaaatat ctaccaccat tatctttttc agcatatgta
    26461 acttccatat ctccataatg tttctttata gattctataa tattaaaata taatttctct
    26521 aatgtcattt ctggagttag agcctttcta gttgcttcta ttacaatagg tgtacttacc
    26581 atatctattg cttttacttc ttttcctgta gcatcacgaa taactttagc aaatgttaaa
    26641 atagacccca tatcagttaa aattaaaatt ccctttcctt tatcaatttt attggctatt
    26701 tctacagctt gtactaaaat atcctgaact ctatcttcta atcccatatt aattgccact
    26761 gcatggttac aatccagtaa agtattggct acattcacca tgctagatgc tgtagattca
    26821 ccatgcatta tgactaatat tgctatactc tcctcattta atcccacata cgataaataa
    26881 agaaatgttg ctaaaaaaat tgcttcatct tctggaaact ctactccata cattaatgac
    26941 atgttagata atatttcttt tgctatgtta tattcatatg ctctttcctt cataatttca
    27001 tctttattag gatgctttac tatatttcca atctttattc tttcaattaa attctgaata
    27061 tgcaaagcta tagcttttat taatttatct aatccttctc ttttgctcca tttatcatgc
    27121 ttttgtaaaa tatattttac agtagttgca attgaatcat taacaatgtt gttataagct
    27181 gtttgattac tactattata tataaaagta ttcattaaat tataggaata cttctgtatg
    27241 tctccatcta taacacttct aatttgtgac tctttaatac cttcttgttg aagtttattc
    27301 caagtttctt ttatcattcc ataaaaatct aaatcatatt ttttattttc tattaataaa
    27361 ttttcacttt caaaatcctt ttcttctggt aaaaaaataa tatcatcttt tccaataaaa
    27421 ctgaaatttt gtacaaactc ctcttttcta ttatttccat ataatgattc tctaatatta
    27481 tttggtagaa gagagagcct tacatataca cattcccttt tataggtcat ataatctaaa
    27541 aaagctcttg cacaaattaa ttgtatatcg ctttttaatt ggcctatatt tcccttacac
    27601 ttatataaaa taaatgcctt aataacttct tttgaaacct ttaacttaac accaatccta
    27661 acactctctt ctttaaaaaa ataacatata agcttcattc tctcttgaaa gcctctttcc
    27721 tctaaagacg gaatctttat agttacagat attcttctta aaaacgtttc caacattgct
    27781 gtttctgggt cttctgtagt ggctgcaatt atcataactc tacactcgtg taccttatta
    27841 gcctctccta accttctata tatcccttta tccataagca gaaatagcat ttcttgtccc
    27901 tctgcagata atctatgaac ttcatctaaa aacaatactc cattattagc ttcatgtaca
    27961 ataccatact tatctttatc agctccagta aatgctcctt ttacatatcc aaaaagctgc
    28021 gctaataata attgctgatt atcagaataa tcagcacagt taaatatgat aaatggtgca
    28081 ttatcactat cttcttttat agttaaacta tacttataca tatattctgc aaatatagat
    28141 ttaccaactc ctgtaggtcc tattattaat gtacttaacc ccttaggtgg atatattact
    28201 gctgctttag cctgttctat ttgggacctt aaacttccat cacatcctac taaattaaaa
    28261 atattgttct ctttttttat actctctaaa atatactcat ctttgataga atcattttca
    28321 ctatattcgt ttatagagtt aatattatca aatatccctt tatttagttt ctttcctaaa
    28381 atactctcaa tactttcttt atataagtac tgaacaggct taccctttat ctttataata
    28441 atactttctt ttattaattg atttaattcc ttactagcat tacttctatt tatacctact
    28501 tcagtagcta tatattctgc atcaaatcca aatctaatgc cattattaaa gttcaagtgt
    28561 tcttctgtta accttttcaa gcaaattaaa actttttctt tccttgatag cttcttttgt
    28621 gtcatagtta acctccccta taagcagtaa tactgttaaa catttattat taagcttaat
    28681 tctacaatat tttaatattt ttgtctaatt atgtaactat tgattttctt tcctattcgt
    28741 tttataatat aaattacgtt accctcatct tttatagagg aggaatttat atgaattttt
    28801 tatcagattt acacacccac tctatagtca gtggtcatgg ctatagcact ttactagaaa
    28861 atattaatta ttgtaaagaa aatggaatta agatactagg tacatctgaa catggtccta
    28921 agatgcctgg ttctccccac aaatggtact ttcacaacat tcaaaacatc ccaagaatca
    28981 taaataacat tataatttta agaggctgtg aagcaaatat actagatatt aaaggaaaca
    29041 tagacctaga gccttttgta attcctcggt tagattatct tatcttgtca tttcacgaag
    29101 ctgttttttc tccaaataca ttagagaata atactaaagc tttaataaat gcaataaata
    29161 agcatgataa tattgaaata ttaggacacc ttggaaaccc aaactaccca atcgactatg
    29221 aattaatcat taaattagct atagaaaaga atatcttaat agaaataaat aattgttcta
    29281 ttaaaggcgt gtcaagaaat ggtagctcgg ataactgtaa gtatattgct acactttgca
    29341 aaaaatatgg tgctaaaatc atactaactt cagatgctca catttgtttt gacataggta
    29401 actatgagta ttctgaaaat atcttaaagg aaattaattt tccagatgaa ttaataatga
    29461 attatcctaa aaaacttatt aatcattttc accaaaaagg taaattacta gatgttgatt
    29521 atacaaatat ctaagtaata atacatgctt actttaactc ccataaaaat tttaatacta
    29581 tttatattat atgaaataaa gaaattataa taaagaagct atttaataaa gcttaattac
    29641 ttttatgaac ttgatggatt gatatggtct atgatagcaa tagaaattat aacattttta
    29701 ataagcttag ttttatatac cttatagtat aaatctaaaa aagcatcatc taaaaaggag
    29761 ctgtcgcatg agcaaattaa atttgttcta tgatagctct ttctttactt attttaatta
    29821 ggtatatttt taattatttt atataaagct tatgttctaa gtaaaattat gcataacaaa
    29881 caaaggtata aaaataccat tttttcagat aatatttaat tataaataat aaatttagaa
    29941 tacttctttt aaatgatgag agtctagaaa tttttatgtg tattctttaa ataaaaattt
    30001 ccagactcta gccccttttt gaatatttta aaatttaact taaactaagc aaattctata
    30061 atagcttatc
//
LOCUS       DAEMHZ010000039        29863 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000039 DAEMHZ010000000
VERSION     DAEMHZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.039"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(85..927)
                     /locus_tag="KPK72_001619"
     CDS             complement(85..927)
                     /locus_tag="KPK72_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2488847.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1502..2404)
                     /locus_tag="KPK72_001620"
     CDS             complement(1502..2404)
                     /locus_tag="KPK72_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2488848.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            2759..3937
                     /locus_tag="KPK72_001621"
     CDS             2759..3937
                     /locus_tag="KPK72_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2488849.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            3946..4602
                     /locus_tag="KPK72_001622"
     CDS             3946..4602
                     /locus_tag="KPK72_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG2488850.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            4602..5267
                     /locus_tag="KPK72_001623"
     CDS             4602..5267
                     /locus_tag="KPK72_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG2488851.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            5372..6148
                     /locus_tag="KPK72_001624"
     CDS             5372..6148
                     /locus_tag="KPK72_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2488852.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLDEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            6210..7544
                     /locus_tag="KPK72_001625"
     CDS             6210..7544
                     /locus_tag="KPK72_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2488853.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            8040..8966
                     /locus_tag="KPK72_001626"
     CDS             8040..8966
                     /locus_tag="KPK72_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG2488854.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKQELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(9358..12897)
                     /gene="nifJ"
                     /locus_tag="KPK72_001627"
     CDS             complement(9358..12897)
                     /gene="nifJ"
                     /locus_tag="KPK72_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG2488855.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAVPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTDEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(13415..13984)
                     /locus_tag="KPK72_001628"
     CDS             complement(13415..13984)
                     /locus_tag="KPK72_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG2488856.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(14056..14664)
                     /locus_tag="KPK72_001629"
     CDS             complement(14056..14664)
                     /locus_tag="KPK72_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2488857.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(14672..16435)
                     /locus_tag="KPK72_001630"
     CDS             complement(14672..16435)
                     /locus_tag="KPK72_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG2488858.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYLTEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(16478..16945)
                     /locus_tag="KPK72_001631"
     CDS             complement(16478..16945)
                     /locus_tag="KPK72_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488859.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            17128..17589
                     /locus_tag="KPK72_001632"
     CDS             17128..17589
                     /locus_tag="KPK72_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488860.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(17682..17852)
                     /locus_tag="KPK72_001633"
     CDS             complement(17682..17852)
                     /locus_tag="KPK72_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488861.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(18041..18262)
                     /locus_tag="KPK72_001634"
     CDS             complement(18041..18262)
                     /locus_tag="KPK72_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2488862.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(18346..19572)
                     /locus_tag="KPK72_001635"
     CDS             complement(18346..19572)
                     /locus_tag="KPK72_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2488863.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(19634..20566)
                     /locus_tag="KPK72_001636"
     CDS             complement(19634..20566)
                     /locus_tag="KPK72_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG2488864.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIAAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(20655..21182)
                     /gene="hpt"
                     /locus_tag="KPK72_001637"
     CDS             complement(20655..21182)
                     /gene="hpt"
                     /locus_tag="KPK72_001637"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2488865.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            21411..22487
                     /locus_tag="KPK72_001638"
     CDS             21411..22487
                     /locus_tag="KPK72_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2488866.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(22573..23781)
                     /locus_tag="KPK72_001639"
     CDS             complement(22573..23781)
                     /locus_tag="KPK72_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG2488867.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(23942..24331)
                     /locus_tag="KPK72_001640"
     CDS             complement(23942..24331)
                     /locus_tag="KPK72_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG2488868.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(24435..25436)
                     /locus_tag="KPK72_001641"
     CDS             complement(24435..25436)
                     /locus_tag="KPK72_001641"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG2488869.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELLPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(25495..25750)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(25884..27074)
                     /locus_tag="KPK72_001642"
     CDS             complement(25884..27074)
                     /locus_tag="KPK72_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2488870.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYKMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(27355..28476)
                     /locus_tag="KPK72_001643"
     CDS             complement(27355..28476)
                     /locus_tag="KPK72_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG2488871.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(28537..29022)
                     /locus_tag="KPK72_001644"
     CDS             complement(28537..29022)
                     /locus_tag="KPK72_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2488872.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(29024..29692)
                     /locus_tag="KPK72_001645"
     CDS             complement(29024..29692)
                     /locus_tag="KPK72_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488873.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
BASE COUNT        10061 a         5037 c         3584 g        11181 t
ORIGIN      
        1 ttattttata ttaaatttta aataatatat taaaattgaa cataatatat taaatctata
       61 cattctgtat taaactactc tttattataa attcagtaca gatataactc ttttgatatg
      121 taatgttaaa taaagtagtt catcttgact tacatagtaa tcataattct tttctacatg
      181 agtttttatt ttatatgcac aatcataatc cttttcatac tttactttaa ttatatctat
      241 aatttcatta tttgtatcat tgattttttc tttttttact aatcgttttg caaagaattt
      301 taaatgagtc agaagcctat cataatttat atcatcttct ttaaattcaa ctctataata
      361 atatcttata atatttagta tgtcttttac aattttagta attaatagtg attcctttga
      421 acttccctta tagtttgaat ttactatatg cattgctata aaaccagctt catctactgg
      481 aaatttcaca tcaaattctt tatttatata atctacagcc catttcccaa tctcatattc
      541 ttttttatgt attcttttta tctcatgtag taaatcattc tttatagtta tgttttcatg
      601 atatctttta attgcaaatg caatatggtc agtaagtgct acataaatat attcataaag
      661 cttagtattc aaattctcct ctgcataagc aattatttca gtactaacct caaaaaattt
      721 ctcatcaacc tggcttgcta gtttactaat tttatctaaa aagctttcat cttgtattac
      781 aaaagttctt tcaattttac ttttatctat ttcttgacct ttcttttttt gaaacccaat
      841 tccacatcct gttaatatta attctttttt tatattttca tctaaaacta atacaacatt
      901 attattataa atttgttgaa taatcataat caatatctct cccaaaatac aatttccctt
      961 attcataaaa aaatacctaa attaagatac aattcacctg tataccttaa cctaggtatt
     1021 gcctgcttac cagtaacaat ccagcttgtt aacgcttata tattgtattc aatttatggt
     1081 tttattctat tataaataaa tatatatgtc aagtaaatta ttgaaaacta tttcccttat
     1141 gatttttcat tactcagttt tgaaatagct tctttaaaaa ttagtttata tatctaatta
     1201 tcagaatgct ttctactctt atacatcact ttttaaaaga gctattataa ctctactaat
     1261 ctttctttta gtctctttac tttctaaaaa ataaacttac catagttcct aaagttattt
     1321 atattaaaat taaaaatatt tttgatatct taaaaaatta atgctttata aattctaact
     1381 tttgataata ctttacatta aatagaacgt attatcactt aattgatatg taattaaaaa
     1441 attaaaagtt cactttaaat aaaactgaat ctttttattt tgtgttattt taataacatt
     1501 tttaagagtt ctttttacta aagaaatcta taataaattt aaatacatgt tggtctacat
     1561 ggctataacg ttctttttta ggtctacata atataacttt ccatgtaata ggatgattaa
     1621 aaggcttttc tataattttg ctattttcaa aaaaatcatg aactggtgat ggtagaattg
     1681 ttataaaatc agaatttgtg gttgatagca acaaaaaatc ccatgaacca gacattatat
     1741 atctttttaa cttaacattt tcatcagtaa atttttgcat tagcttatga tgtatcataa
     1801 aagtaggatt aaatattgca agtaactgct cattcaaatc gttccaatct atcttatcct
     1861 tcttagcaag tggatgattt atatttaaaa atgctgtaag ttcatcttct tgaagcacat
     1921 gttcagttac tacactttta tcaattttat gtggatgtaa caatactgca taatctaatt
     1981 cctgtaagat aagcattcta tttaagtcat atgcaccctt ctctactata tcaaattcta
     2041 tatctggatt atttgcaact aactgtgcaa ctacatctga aaatgctatt ccaagtataa
     2101 gtggaggtat tcctattcta acttttcctt taaattgaac tgaatcttct cttaaatctt
     2161 ccatcatatt tttgtattca tttatgatat gttctgcatt tacataaaat ctttctcctg
     2221 ctggtgttaa cccatttaac ctgcctttat acctttcaaa taaatataca tcttcacttt
     2281 cttcaaaatt ttttataatt tgacttaatg ctggttgaga tatgtgaagt gtcttagatg
     2341 ctattgataa attaaaacca gatttcacta tttcaacaaa atatttcaac tgttttatat
     2401 ccattttaaa cccccttccc caaaagtata atttagatta tattttttct tctaatgata
     2461 ttttatagga aaatcaacat aatttcatta atattttaac aaaatatttt tctaaatcta
     2521 ctataaattt ttatcctata aatttttctt atacactata taaaaaatta gacttagaca
     2581 aaacgctttt ttcgccgtaa tctataatta gaaacaggaa aacctaaagc attaggaata
     2641 aaagtaattg aaatacttaa aaacaagctt gtattaaaaa agtactttat tatagtgttc
     2701 cctaatttct ttattcagaa aatttttaat tatcattcaa gaaaggtaac tattagatat
     2761 gaaagatgta gtaatcgtat cagcagtcag aacacctata ggaagttttg gaggtgtatt
     2821 taaaaatact tcagctgttc aacttggaac tatcgctgtt aaagaagcga tatcacgtgt
     2881 aggacttaat ttatctgaaa tagatgaagt aataattgga aatgttcttc aaacagggct
     2941 tggacagaat gttgctagac aaattgctat aaatgcaggt ataccaaatt ctgttccaag
     3001 ctacacagta aataaattat gtggttcagg gcttaaaagt gtccagctag ctgcccaatc
     3061 aatcacatct ggtgaaaatg atgtggtaat tgcaggtggt actgaaaata tgagtcaagc
     3121 tccttatatc gttccaactg ctcgctttgg tagtaaaatg ggtgacataa ccatggtaga
     3181 tagtatgcta acagatggcc ttattgatgc tttcaatcag tatcatatgg gaataactgc
     3241 agaaaatatt gcaactaaat ttgaatttac tagagaaatg caagataaat tggcattaga
     3301 aagtcaaaat aaggctgaga atgctataaa aaacaatcgc tttaaagaag aaatcgttcc
     3361 agtagatgtt ttaatacgtc gaggaaaaat agaaactatt gataaagatg aatatcctaa
     3421 attaggtatg acatttgaag gtttagctaa acttaaacct gcattcaaaa aagatggcac
     3481 tgtaacagct ggaaatgcct caggtattaa tgatggtgca gctatgttaa tactaatgag
     3541 ccaacaaaaa gcagacgaac taggtattag accacttgct aaaataaaat cctatgcttc
     3601 agctggtgtt gaacctgaag tcatgggtac tggtccaatc cctgcaacta gaaaagcact
     3661 gaaaaaagct gggcttagca tcaatgatat agacttaata gaagcaaacg aagcgtttgc
     3721 tgcccaagca ttagctgtta aaaatgaact tcaaatagat tcatcaaagt taaatgttaa
     3781 tggtggtgca atagcattag gacatccaat aggagcaagt ggagctagaa tacttgttac
     3841 cttaatttat gaaatgcaaa aacgtaaagt tgaaactgga cttgcaacac tttgtattgg
     3901 tggtggtcaa ggaatatcaa tggtggtttc acgataggag gaaatatgaa taaaatagtt
     3961 agcattgatg aagctctatc tcatgtaaaa gatggagata caataatggt tggtggattc
     4021 atggccaatg gttcaccaga aaatttaatt gattatttat gtagtaaaaa tataaaagat
     4081 ttgacactaa tttgcaatga tactggcttt attgacaaag gagttggcaa aatggttgta
     4141 aataagcaat ttaaaaagat aattgcttct catgtaggat taaataaaga aactggaaga
     4201 caaatgaaca acaatgaaac agatgttgag ttagttcctc agggaactct tgctgaacaa
     4261 atacgtgcag ctggttatgg tctaggaggt atactcacac cgactggtat tggtactctt
     4321 gttgaggagg gaaagcaaaa aattgttgtc gatagcaaag aataccttct tgaaaaacca
     4381 atttatgcag atgttgctct tctatttgca tcaaaggtcg ataaagcagg taatctagtt
     4441 tataaaggct ctatgaacaa ttttaataat ttaatggcat ctgctgccaa aattacaata
     4501 gttgaagcag atgaaatcgt tgaaattggt gatattgacc caaatgaagt taatacacca
     4561 ggtatttttg tgaattacat cgttgaagga ggtaatttat aatggataaa ttagaaatgc
     4621 aagaatatat tgcaaataga gtatctaaag aattaaaaga cggagccgtt gtaaaccttg
     4681 gaataggtct accaaccaaa gtagcaaatt atattccgga taatgtaaac gttatcttac
     4741 aatctgaaaa tggcttttta ggtttaggtt cagccgaaga tggaaattca agtgatgaaa
     4801 ctattgtaaa tgcaggtggt caaccagtca ctatacttcc tggaggatgt ttctttgata
     4861 gtgctacttc ttttggaata atcagaggag gtcatgttga tataactgtt cttggcgcac
     4921 ttcaggttga caaatatggt aatatagcaa actatatgat tccaggtaag atggttccag
     4981 gtatgggtgg agctatggac ctagtaactg gagctaaaaa agttattgta gcaatggaac
     5041 atacttcaaa aggttctgca aaaatattaa ataattgtac tcttccactt actgcaacaa
     5101 atgctgtaga tttgatagtt actgaaatgg gagttatgga agtaacatca gatggaattt
     5161 tacttaaaga aataaatcca gctttcacat tagatgatgt tatttctgct actgaagctc
     5221 ctttaatatt gtcagattca ttaaatggaa aaatatctgt tgtttaaaat aattaaaact
     5281 agaaaataaa aatctcttag acgtattaca tataaatttg tattgcttaa aatattaata
     5341 taaaaataaa ataaaattgg aggaaatgaa aatggttaaa gataaagttg tattcgtaac
     5401 aggagctgca agtggaatag gtaaacaaat agctgaatcg ttcttaaaaa atggtgctaa
     5461 agtaatgttc tctgatataa atcaagaagc tttaaataat accataacag agttacaaaa
     5521 aaaaggttat gattgcatga gtgtaaagtg tgatgtaact aaggaagaag aaattaacca
     5581 cgctatagac aaaactgttg aaaagtatgg cagattagat atattaataa acaatgctgg
     5641 acttcaacat gtttctatga tagaagattt cccaactgat aaatttgaat ttatgataaa
     5701 aataatgctg acagcacctt ttatagctac taaaaaagca tttcctataa tgaaaaaaca
     5761 aggctttggt cgtataataa atatggcctc aattaatggt gtaataggat ttgctggaaa
     5821 ggctgcttat aactcagcaa aacatggact tattggacta acaaaagttg ctgctcttga
     5881 agctgccgat tctggtataa cagttaatgc tttatgccct ggatatgtag atactccact
     5941 tgtcagaggc cagttcaatg acctttcaaa aactcgtaat ataccattgg aaaatgtctt
     6001 agatgaagtt ttatacccac ttgtaccaca aaaacgccta ttagatgttc aagaaattgc
     6061 cgactatgct atgttcttag caagtgataa tgctaagggt attacaggac aagcttgctt
     6121 actagatgga ggatatactg cacaatagtt attatattta gggcttgaag aaagtattct
     6181 tcaggtcctt ctactaagga gggatattta tgagtattat cgcgccaatt ggtataatta
     6241 ttggtatcat agctattata tatttttcag ttaaagaaat tcatataact atagctgctc
     6301 caatagcaac tttaatagtt gttattttaa ataaaatgga tgttgtaacg tcaatgctag
     6361 gtgctgacaa aaacaattat atgggtgcac ttggaaacta tataatgagt tattttgcaa
     6421 tattcttatt aggctcaata cttgctaaat ttatggaaga aagtggagct actgtatcaa
     6481 tagctgattt tattttaaat aaatttggct ctaatcatcc ttatagagtt ttagttgcta
     6541 tatttatagt aagttttata ttaacgtatg gtggtataag cttatttgta gttatgtttg
     6601 cggtcattcc acttgcaaga actctattta aaaaacttga tatttcttgg aaccttattc
     6661 agataccact ttggcttggt attgcaacaa ttactatgac tataattcct ggaacaccag
     6721 ccattcaaaa tgttatacct attcaatatc taaatacgtc tcttactgca gctactatac
     6781 caagtattgt aggtagtata ggttgtgcta cgtttggact tgtctatatg aaatatgctc
     6841 taaacaaaag tattagaaaa ggtgaaactt atgcaactta cacttcagaa ttagaagaag
     6901 aatctttaga tagagaatta ccaggatttt ttgcaagtat ttttcctcta ttagcactcg
     6961 taattatagc tataactgga agtacttttg gttcagaatt tatgaaaaaa aatgtaattt
     7021 acatagcttt aatagttgca attcttttat ctgctttttt attccataaa tttctatcat
     7081 ctaagataca gacattgagc ataggtgcta gtggttctat aggtcctata tttgctacat
     7141 cttctgcagt tgcatttgga gcagtaataa tggctgcacc tggttttgat gtatttagca
     7201 aacttataat gtcaatacca ggtagtccac ttataagtct tactgttttg acttcaacaa
     7261 tgtcagcaat aacagggtcc tcttctggtg cattaggaat agtaatgcca aactttgcag
     7321 attattattt aagtactggt ctaaatccag aattaattca ccgtgttgct acaatagctt
     7381 caaatatact aactatagtt ccacaaagtg gtgtttttct tacattctta agcttatcta
     7441 agttaacaca taaaactgga tttaaagaaa ctttcattgt agttgcagtt ggttcactaa
     7501 tagcagaaat tattgtaatt acattaggat tactaatgta ttaatataaa acaaattaaa
     7561 attcataata tgaaaggtgg tgtctcaaag tgaacttttt acttaatgag acactctttt
     7621 tatatgaaat aacatatgcc tatcatttca acaataaaaa aggccttatc tctattttga
     7681 gacagaccct ttttattttt taaagattta aataattata atttgttgct aatacgaaag
     7741 catctactga ataaatttaa ccaaataaaa aaaaatttat cataactata aatttacgtt
     7801 atggaattat tataaaaata ttattttctc taactaagta attccatact atacaggctt
     7861 ttaatacaaa ttgtcaaaca cactgaaata tacctattta ttccatatat atctttttta
     7921 catatcttta cagtaaaaaa agtcttaatc ttatgttagt atagattcac gggtaacccc
     7981 ctacactaaa ggttacaaat cagtaacatt tttagaattt ttagattaag aggtgtttaa
     8041 tgatgagaag aaatatacca acaaaattaa aaactatatt agctttagga gtgttaactt
     8101 tagcaacagc ttcaagtgct gttactgcaa gtgcttcatc acttgaaaat aatgtaaata
     8161 taagtaacaa aagtttagta tatagtaata ctaaatacaa gttttctgca aaaccaaatt
     8221 gttctgtaaa acaagaactt aagcctgata aagataactc ttgtaaggat aagaatcata
     8281 atgacaagaa ttgcaacaac actaataaac ctgaggacaa caataattca gactctacta
     8341 ataagcctga taacaataaa cctgagtcta ataaacctga agacaacaat aattcaggct
     8401 ctactaataa acctgataat aataaacctg agtctaataa acctgaagac aacaataatt
     8461 caggctctac tgataaacct gataacaata aacctgagtc taataaacct gaagacaaca
     8521 ataattcaga ctctactaac aaacctgatg ataataacaa ctcaggctct acaagtgaaa
     8581 gtttttcagc ttaccaaaaa gaagttgttg acttagtaaa tatagaaaga gctaaagctg
     8641 gattaaatcc tcttactctt gattcttcta tatcaaatgt tgctacaaaa aaatctcaag
     8701 atatgattga taacaactat ttttcacaca attctccaac ttatggttct ccatttgaca
     8761 tgttgaaaaa atttggaata agctataaga cagcaggtga aaatatagct atgggtcaaa
     8821 aaactcctaa agaagttgta aacgcatgga tgaattctga aggtcacaga aaaaatataa
     8881 tgaatccaaa tttcagtaaa ataggtgttg gagtagcaca aaaaagtggt ggttcaattt
     8941 attggacaca aatctttgta ggataaaata ctctttttcc atacatatca aaagagtatc
     9001 tctaagtgat aaaaaacaca aatgagatac tcttttgtat ctattttaaa attatcaaat
     9061 ccaaatatac atactataaa tattatttgt tctcttaatt ttagtgtgta gagcaatata
     9121 taaaaagtat tggcaagatg aaaatggaaa acttaaaaag atatttaata ggtaaaaaca
     9181 ggatcttaat atcttcataa aaaaaggctg tctcaaaata gagataaggc ctttttcatt
     9241 gttgaaatga ttaaaatata tttgatttca tacaaaaaga gtgcctcatg aactaaaaag
     9301 ctcactttga gacactctct tttatcaatt ctatataagc attataatac tttaatatta
     9361 ttcagctaat tttttgtatc cttcatatct ttctttagca tcagcttcag ctttagcaaa
     9421 taatccatca gcttcctctg ggaattgttt agctatagca gaatatctta cttgtgcaag
     9481 tatgaaatct ttgaagcttg cagttggttc ttttgaatct aaagtgaatg ggtttttacc
     9541 ttcagctttt acttctggat tgaatctgta taaatgccag tatccgcaag caacagcttg
     9601 tgcttcgtta gctacagttc ttcccatacc ttcttttaat ccatggttta tacatggagc
     9661 ataagctatt attagagatg gcccatcatg tttttcagct tctataacgg ctttgataaa
     9721 ttggttttta tcagcaccca tagcaacttg agctacatat acgtttccat atgtcatagc
     9781 catcattcct aaatctttct ttctagattt tttacctgaa gcagcaaact tagccatagc
     9841 agcagctgga gtagctttag aagcttgacc tcctgtattt gaatatactt ctgtatcaaa
     9901 tacaagtaca tttacatctt caccagatgc aagaacatgg tctaatccac cgtaaccgat
     9961 atcatatgcc caaccgtctc cacctagtat ccattgagat ctcttaacta gataatcttt
    10021 tctatctttt atagctttta ctaattcttt agctttttca tctgtacaat cacaagctgg
    10081 tttttctaat aattcaacaa ccttagcact tgctacttta gaagcttctc catcatttct
    10141 agaatctaac cactctgtaa atactgcttt agcatcttca cagatatcca tagaaactaa
    10201 ctctgtcata gcatctacta ttttatttct tatttgctta acagctgtgt acataccaaa
    10261 tccatattca gcattatcct cgaataaaga gtttgcccaa gatggacctt taccttcatg
    10321 gttacaagta taaggaattg atggtgcaga agctccccat atagaagaac atccagtagc
    10381 attagctatc atcattctat ctccatataa ttgtgtaact actttaatgt atgcagtttc
    10441 tccacatcca gcacatgctc ctgaaaactc cattaatggt tgtctaaatt ggcttccttt
    10501 aacagtgttt gcaggcataa catcaccttt tggagctacc tcatcaggat ttactgcaaa
    10561 tgcccaatta tctaactctg tatcaagttg agtatctatt ggcttcataa ctaaagcttt
    10621 ttctttagct ggacatatgt cagcacagtt tccacatcct gtacagtcaa gtggactaag
    10681 ttgcattcta tattttaatc cttctaatcc tttacctaat gcttttttag cttcaaatcc
    10741 ttctggagca ttagctaatt cttcgtcagt aactaatact ggtcttatac atgaatgagg
    10801 acatataaaa gaacattggt tacattgaat acagttatct attatccatt caggaacatc
    10861 aacagctata ccacgttttt cataagctgc agtaccacaa gggaatgttc catcttctat
    10921 accattgaat gtacttactg gtaagttgtt tccttcttgt gcagtcattg gtcttagtat
    10981 attttttata aattctggtt ctttaacttc tcctttatct gcatcaacag cattttccca
    11041 agaagctgga acttcaactt taactaaagc atttattcct gcatcaacag cagcatagtt
    11101 catgttaact attttttcac cttttttacc ataagcttta actatagaat cttttaagta
    11161 tttaacagca tcttcttctg gtattatttc agctaattta aagaatgcag attgcattat
    11221 catatttatt ctgtttccaa gacctatttc ttgtcctagt ttaacagcgt taactgtata
    11281 gaattttata tcatttttag ctatatattg tttcatatga gctggtaaat gttcttcaag
    11341 tcctgcttgg tcccatatag tgttaagtac aaatgtacca ccttttttaa gaccttttaa
    11401 taaatcatat tgataaacat atgattgttt atgacaggca atataatcag cttcatctat
    11461 taaataagta gctcttattg gagtatcacc aaatcttaag tgagacatag ttataccacc
    11521 agatttttta gagtcataat caaagtaagc ttgagcatat ttttcagtat ggtcaccgat
    11581 tattttgata gcttgtttat tagctccaac agtaccgtca gaacctaatc cccagaactt
    11641 acatcttata gttcctttag aagctatttt tattggctca gatggagtta aagaagtatt
    11701 agttacatca tcaactatac ctactgtaaa tccattttta ggttgttctg aaactaagtt
    11761 atcaaaaaca gtttttatat ctgaaggagt agtatctttt gaacctaatc cgtatcttcc
    11821 acctattatc ataggagcat tttcttttcc atagaataca tcacgtacat ctaagtataa
    11881 tggttcacca gttgaacctg gctctttagt tctatcaagt acacatattc tttcaacagt
    11941 agatggcaca gcatctaaga aatgtttcat tgagaatggt ctatataaat gaactttaac
    12001 taaaccatat ttttctccct tagcatttaa gtaatctata gtttcttcta ttgtttctgt
    12061 tactgaaccc atagctatta atacgtattt agcatcttct gctccatagt aatcaaataa
    12121 actatgtttt ctacctgtta aattactcat tttttccatg tatttttcaa ctatacctac
    12181 tatattttga tagtatttat tagaagcttc tctagtttgg aaatatatat ctgggttttg
    12241 agcagtacca cgagcaactg gatgatttgg agataaagca ttatctctaa atgcttgaac
    12301 tgcttcaaag tttaataaac ttgcataatc ttcattttct agtaattcaa ctttttggat
    12361 ttcatgtgaa gttctgaatc catcaaagaa atgtataaat ggtaatctac cttctattgc
    12421 agctaaatgt gcaacaggtg ctatatcagc tacttcttga actgaaccag aagctaataa
    12481 tacacatcca gtttgtctag cagccataac gtcttggtga tccccaaata ttgataacgc
    12541 ttgagatgct aaagcacgag cacttacatg gaatactcct ggtaataact ctcctgcaac
    12601 tttatacatg ttaggtatca ttaataataa accttgagat gctgtaaaag tagaagttaa
    12661 agctcctcct tgtaaagaac cgtggaatgt tcctgatgca cctgcttctg actgcatttc
    12721 aacaacatta actgtttgtc caaatatatt ttttcttcct tgtgatgccc actcatcaac
    12781 tacttcagcc atagttgaag atggtgtgat tggatatata gctgctacat ctgtaaacgc
    12841 ataggcaaca tgagctgcag ctgtatttcc atcaagtgtt ttcataaact tagccataat
    12901 atgtatcctc cttgatagct acaattgtac ttgtttataa caagtctatt tttttatatt
    12961 tacatataat ttacacaaca aaaatataaa cacacatata gtcgcgcaaa tactctatct
    13021 tctaatataa tactacagat tagataaaat tcctttgaaa attgtaaatt aattacatat
    13081 ttaagtagat tttttttaaa attaaatatg ttaagttttt ttcataattt tgtatacaat
    13141 atataataaa ttaagttaga cttttcaaac ttttttatct atttgtttat attagtacaa
    13201 aaagtaaaat aattatcttt attatatgta aatttttata tatataattt taccatatta
    13261 catcatattt ttgtaagttt ttattacatt ttttataata cccctttatt ttataaatta
    13321 atctaatcga ttaaatttat cctatagaaa tttattacat ttaaaaatat gctagcaaat
    13381 aaaatatcta ctagcataaa gtctatttat aatattaatc aactattaat tctactaaat
    13441 ctccattttt aacgcctgct gcatttccct cttccatatc aacatgcatt tctagattaa
    13501 agctgtcact agctcttact aatacatttt caaatactaa tgctcttggt ccagatgttt
    13561 taacttttac tatttgctta tcagttacac caaatttagc agcatcttca ttgctcatat
    13621 gtatatgtct agaagcaact ataactcctt ctttcatttc aacttctcca gcaggtccta
    13681 ctaatttaac acctggagtt ccagctatat caccagattc ttttattggt gcttttactc
    13741 ctaatgggaa accatctgct aaagatattt caacttgagt atgagctcta gctggtccta
    13801 gtactctaac tccttttaaa gttccttttg gtcctactaa atccactttt tcttcacagg
    13861 catattgccc tggttgagat aatggtttaa atttagttaa ctcatgacct gctccaaata
    13921 atacgtctat atcagcttga ctcaaatgaa catgtttatt agataatgct attggtaatt
    13981 tcattgaaat tccctccttt taattattta aattattgca atacaattat acataatatt
    14041 acctacaaaa tcaaattata ttgaatcaat atatatcttt aaatttttat atattatatc
    14101 tttaaattct tgattatcta tataagatac atcatctgta ttgtttaaat atatattgtg
    14161 taaaagttta gtatttatac tcttaaagaa aaaatccata acaatctgtc cacctttaaa
    14221 ctgtgaatca tatgatttac tccctcccac tgcaatatac attcctatcc gtttcttact
    14281 tctatcaatt gaaggtttat ttaatacata tttacttgca tatatagctt gagttctatc
    14341 aacaacagtc tttaatttag ctgatattga atcaaaatgt acaggactta ttactatagt
    14401 tccttttgaa tcatcaaaca ttttatataa tggcttcata tcatcattaa acttgcaata
    14461 ccctgttttt tcacaaaatc cacatgctgt gcaatattca atatccatat tgtagatatt
    14521 aaatatttta caacttaatc cacactcttc tagcatagct tttacttcat ttgctataaa
    14581 actgcaattt ttattaattc ttggacttcc atttattatt ataatctctg gacttttaag
    14641 tcctttttta tcgtttgaat ccataatcaa tctacctatc ctttaacata actattaaaa
    14701 tatcatcatc ataattatct tttgatgaga agttctttaa atccaatttt agattttcta
    14761 ctattaactg ttgatttaat ctaaaatttt cttttagaaa gttttctaat cggtttatac
    14821 catactcttc ttttgatgaa ttttttatct ctaaaattcc atcagtgtac atacatacca
    14881 ttgcataatc ttccattgaa agagtattat tttcataagt agcatcttcc attattccaa
    14941 taggtatgcc ctttttacaa tttaaattat tttctattgt gccatctttc tttacaacta
    15001 caggactata gtgacctgca ttagacacag taatcatatt ttctttagta tctataatac
    15061 ctatcaaaca agttgtaaat actcccatct tgtcaaattc atcatataac atcctattta
    15121 aatttgtcat tatttctctt ggtgtattgt attgattgca taatacttta aaagcacctt
    15181 ttatcattgc aacaacataa tttgatacca ttccatgtcc cattacatca gcaacaatat
    15241 aaactatatg ttcatcatcc acttttgtag cataataaaa atctccacct actacccttg
    15301 ctggtttatg gtaaaaataa agttctttct catcattaca atccatttta cctttatcca
    15361 taataagctg ttgttgttta tttaatatgt tcaactcgtt ttcaagaact ttgtgtttta
    15421 cttccttttc attatgttca tataattgca taccaacaga aacttgtttg gacaatatac
    15481 taagaatatt taaatcatca ctagtatagt ttttgctatt tgcacaaact atacatccta
    15541 tagtctcatt tttgtcctga agtttgtaat atatataact ttccaaattt attccctttt
    15601 ttgagcatgg ttttaaatta tttaaatacc cccccaatga ctctttcttt aaaaacatat
    15661 caatcatagg atataaattt gttttctttt tttgctcaac attataacaa ctaactatag
    15721 catccttatt atgatgacta tttataatta ttaaagcttc tttactatta gtaatattac
    15781 acgcttgttt acttaaattt tccaaaaatc catcaatact cttattaagg tatagttttt
    15841 tagtagcttc atttatggca cttattgagc ctcttagttt atcaattaaa atttgtacat
    15901 tattattttt attagttatt tctattgata aactcaagag tgatgcaaca gatgatataa
    15961 aatctatatc ttgttctgtt agataatcat cctcttttaa aaaacaagtc ataaatccaa
    16021 caactacatt atttattata aaaggaaaaa ctattctccc aatataacct tcagcttttg
    16081 ctaaatctcg ttcaccttct gctctagaat cttcaaatac attttccaca tatattatct
    16141 ttttttcatt tacagcctca tgtatatact ctggatatgt gtcaaaatca atttttgcac
    16201 ctttaagact atttattgga aaaatttgag gtacatattc taaggtttgc gaacatacta
    16261 agtaggcata tttagaatca tttttataaa atagattcac acatgccttt gtagggtgaa
    16321 caacttctaa cattttttct attatgtcat cttttatatt aaaaaaatct gtagattcag
    16381 tcaccatagc ggatatttcc actaaattct tcattttata actgccatta gtcatactca
    16441 atttctcccc ctatatatta aactatttat tagtatttta acatacattc tttatagtaa
    16501 aaacaaaaac aacgacaaaa ctagcaacta atatccagtt aaatcctgtc aataatccta
    16561 taagaagtaa tgtaattaat gcatttagac ctgttctccc tttttcatcc ataaaagtta
    16621 ttttagagat gtcgatatta tttttcacat tatttatatg ctcttttact tgttcttcca
    16681 tatttctttt atatagtata taaactacac caactaaaaa cactcctata cctaaaacca
    16741 tgtttaaatt aaaagctacc ataagcaata ttgaaccaaa agctaacttt aaaagcttaa
    16801 aaaatacact atctatcttt agagttatat ttctgatttc tttacattca tctcttaaag
    16861 aatatccact tgaatctaag ttattaatct ttgtttttac aatttcagaa taaaaaagct
    16921 ttattatccc atttaacatt ttcatcactc ctaaatattt tacatatatt tgtaagtttt
    16981 acaattataa tattttttat acttggtaat aataaaaaat aacaaatatt ttatgattta
    17041 gtatttattg taaataaatt attgaagctt tattagttaa gtatagattt ttaataaata
    17101 tttttataat tagaaggaag tggttttgtg caaatagaac aactcgattt agaaacgaga
    17161 aataaaatat actgctatac taaaaaaatt cttagaaaat atcaaaaagg cataacatct
    17221 ggaaaattaa cagcagataa atttgcagat aatatattgt ctcaagattt tatcagtagt
    17281 atacttaatg aaaaaatagt caatgagaca aactttaaga tatcttatag aaactatata
    17341 gaaactttaa taaatattca aaatgaaaat ttatctaact taagaaaaaa atctactaaa
    17401 actgcaaaat gtttcaatat ctctcaaata acccaattaa aaaacttact aagtaatact
    17461 ggttataatt tattaattcc atataaatac ttaactgcta gagatataga aggaatcgtt
    17521 actcttatta atactgggtc tattgaactt ggaaatgaga gaatctacaa ttatatatca
    17581 aaagcataat aatttaaacc tattataaaa aggcaattta tttaataaaa aatattttta
    17641 atattaaata aattgccttt ttaattttgg actaaatact gctatccaag tgctttttct
    17701 agtttagata aatgttgtat tggataatat tttagtagct ctttaaattg ttctactgat
    17761 aaaccttctg ttgttgtata tattcttatt ttttcatcta agtcactatt aataaatttg
    17821 ttttttaaat cctcaaaatt tacactactc attttacatc actccttatg taacatattc
    17881 atttgtatta ttttatccaa atacataaaa tttattacat aaaaatatat ctatttaata
    17941 atttaataaa aaaaagctat ctcaatttag aaattacttt ctatatttga gatagctcta
    18001 gctaaactaa tagccaatag ttataaataa ttatttataa ctatctgttg ctttttccag
    18061 ccatttgttg ttcagccatt tcaactaatt ttttagtcat gtatccacct acataaccat
    18121 tttctcttgc agtaaggttt cctttatcaa cttgttgata attactcata ccaagttcat
    18181 tagctatttc taatttcatt tggtttaaag cctcttttgc tcctggtact actgttctgt
    18241 tgttgttatt gttacttgcc atgttgatta ccttccttct gtttaattta ttttgtgttg
    18301 ctgataatat tatgtgattt taaacatttt taattcaggt aatttttagc aattaaaacc
    18361 tgccaaatat ccagtagaaa aagctatttg caaattgaat ccacctgtat atgcatctat
    18421 atctattact tctccagcaa aaaataaacc ttctactaat tttgattcca ttgtacttga
    18481 atttatttcg tttactttta caccaccaga agttattata gcttcttcaa taggtctgta
    18541 tctctttact gtaaacttta aattttttaa taaatggact aagttttttc tctgctctct
    18601 agaaatttgg tgaacaaccg tatttggatt aatttcactt aagtttatta ttataggtat
    18661 tagttttttt ggaagtaaat catctaatgc tttttcaaat tttttatttg tatacttctg
    18721 aaaatctttt tgaactcttt tatccaattt ttcctcatct aatgcaggtt ttaaatctaa
    18781 taatatagtg taattttctt tccttgtatc tttcattcta caacttgcag acttgattat
    18841 aggtccatct aaaccaaatt tagtaaattc aagttctcca aaatcactat aaactttctt
    18901 ttgtttgcta ttaaacaccc ttatttcaac atttctcaat gataattttt ctaaatcttt
    18961 tgtaaaactt tcttgtactt ctataccaat taaagatggt tttgtatcta taattgtgtg
    19021 cccctgtgaa attgcaaatt tatatccatc accagtagaa ccagtaagtg gataacttaa
    19081 tccaccagtt gctactacaa ctgaatcaca cttaatttct tttttatcgt ttaaaattac
    19141 tttttcaatt ttattatttt tagatattat tttttctact ttcgaattta ataatatatt
    19201 tactttctta cttttaagtt gtctttcaag agcatttaca atatcaaatg ctttatcact
    19261 ctctggaaaa accctttttc ctctttctgt tttagtcttt actccaagat tattaaacat
    19321 acttattaca tcatcatttg taaaagtata aaaagcacta taaagaaatt ttccatttgt
    19381 aggtacattt tctattaatt cctctatttc acaagcgtta gtaatattac atctaccttt
    19441 tcctgttatt gcaagttttt ttcctaattt atgatttttc tcaattaatg ttacatcata
    19501 gcctttctca catgcagttg atgcggctat catccctgct ggtccaccac ctattattac
    19561 taccttcttc aaataatcac tcttttctac ccttaatttt tttaatttaa actattgact
    19621 ctatacctta aaatcaattt aattttaaaa gtttaaataa tgctggagct cctgctattc
    19681 cataaaaaac aactactgta ttcttaataa aattcataaa aattatactc atagtagagt
    19741 cacttatatt taaagtgact attaacattg aaataaacaa cttaatgcca atatataaca
    19801 ttcctattgt tactatccca accaaaaacc tacatattct tgaaaaaatt atatttttac
    19861 tccccttatt tttattgcta aaatttattt ttttgttatt attatctgta ttaaatttaa
    19921 caaatctatc ctcaaccata tatccaaata caaatcctac tattatacca aatttttcaa
    19981 aatattcagg gctatttaga aatatagcca ctattataaa tggtataagt aaaactacaa
    20041 gtaaaatata atttttatta tcatcaatat agtcaaatat ttttacaaat acaactgtaa
    20101 aaaatattcc taatatccaa cccgctatca catctattgg ccaatgaaca gctaaataca
    20161 gcctagacaa tcctactcct aaaaccataa ctgcagctat taaatgaaga tatctctttt
    20221 taaatatagt cataatacta acccaaaatg ttgttgcagt ttgagtatga gcacttggaa
    20281 aagaataacc accagcagta gaaactctca atgattcaag accttttatt cctattggtc
    20341 tgggagcttt tacaaactct ttgatgccta aatttacaac aaaatttcca attatagcaa
    20401 acagtatttt ctgtccacat tttttattta tacaccagta tataattgtt gtaattaata
    20461 ttaataacgg tgcttctgtg cttattgtaa agacaagaaa tataaagttc aaaattggat
    20521 ttcttaaatt ttggaaaaat aataatatat ttaactgaat atccatatag atgtctcctt
    20581 tactaaaatt ttatataatt aactaaaaag cactaactcc cttagaaatt agtgcttttg
    20641 attcacattt aaatctattc tacatcctca actataccta tataaggtaa atttctatac
    20701 ttttcagcat agtctattcc ataaccaaca ataaatttgt cttctataac aaatccaaca
    20761 tagtctacag gtatattagc ttttcttctc tgaggtttgt caagtagagt acatagtttt
    20821 aaagattttg gatttctagt ttttaaagct gcaactagat tacttaatgt taaaccagaa
    20881 tctattatat cctctacaat caacacattc tttccttcaa tatctacatc taaatctttt
    20941 aatattttaa ctgttccaga agactctgtt gagtttccat aacttgtaac actcataaaa
    21001 tctatattta catccaaatc tatacatctt attaaatctg aaacaaatac actagctcct
    21061 ttaagtatac ctaccactag taaatcttcg cctttaaagt cttcttctat cttttttcct
    21121 aattcataaa ctttttcttt tatttgttct tctgtcaaca tttttcctgt aactttgtac
    21181 attttgtatc ctcccgcttt ccttctatta acttatatat tatagcatac caaaatatca
    21241 attaaaatct ttttcatcat ttttaaacct aaattaacag cttatccata cttttttgct
    21301 ttttccatta aaaatttgga ataacgattc ctttgtatta taatctatat gtacatatat
    21361 taatcagtta tttttatcga ttatataatt ttatttttgg aggtactttt atgagcacaa
    21421 atcatggagc taatttatat agtttatcta gtaagtatgg cttttcaaag gaagaattta
    21481 tggattttag ctcaaatata aatccttttg gtacatcaag tttagccaaa caatacattg
    21541 taaacaatat agatatggta tctatgtatc ctgaccctga ttacatagac ttaaaaactt
    21601 ctatatccaa ttactgcaaa tgttctatag ataatatagt acttggcagt ggagctacag
    21661 aactaatttc atcatttata catacaatta atcctaaaca ggctctttta ctctctccag
    21721 cctattcaga gtatgaaaaa gagttatcta aaattaattg ttctatagaa aaatactttg
    21781 caaaagaaga agataatttt catataaatt tagaaaatct tataaaaact atcaatgcta
    21841 aagattatga tttggtagta atctgtaatc ctagtaatcc aactggattt gcatttacta
    21901 aggtagaagt gagagaaatt ttaaaaaata cagactcatt tttaatgatt gatgagactt
    21961 atgtagagtt tactgataca gatacatact cttgtactca attagttgat gattattcta
    22021 atttattcgt tataagaggt acatcaaaat ttttttcaac gcctggaata aggcttggat
    22081 atggccttat atctaacact aatgtcaaaa atgaaataaa taaaaatcta gatttatgga
    22141 atataaacat aatagcttct aaaatgggag aaatcatgtt tagtgattta gattttatat
    22201 caaatactat ttccttaatg aacactgaaa gagattacct gcttaaagag cttaagaata
    22261 taaaaagctt agatatatac aatactaaag gtaatttcat attgtgtaaa atcaagacaa
    22321 aggaattgac tgctaaatct cttcgagaac aactgctccc acaaaaaata ataataagag
    22381 attgttgttc atttgaaggt ctagatgagt atttctttag agtatgtata ttaaaaccta
    22441 atgaaaataa attattaata tcatctctta aagcaatttt taaataaaaa aatcacttta
    22501 ttttataatt ttttatacca taaaaaatga gctatctcta tttgagataa ctcatttttg
    22561 attattaaaa aactaagcac tttgctcttc tttgtttcct tttttactca ttccatcatt
    22621 tattttatct actactactg ctattgcatt atctcctgat acattacatg ctgtaccaaa
    22681 actgtcttga gttaagtata aagctatcat taaacttgca agcccaccat ctgatggtat
    22741 tccaaccatt ggtaggaaag gaagtgcact cattatagcc ccacctggtg ctcctggtgc
    22801 tgcaaccatt gctataccta acattgcaat ataagggaat aatacaccta ttccatgtgg
    22861 cataccattc atcattaaaa ctgcagttgt aaatcctgtt attgatatca tactacctga
    22921 taaatgtatt gttgcacaaa gaggtacaac aaattctctt atttgcttac ttactccatt
    22981 tttttctgca caaccaacat taactggtat agttgctgct gaagattgag ttcctatagc
    23041 tgttaaatat ccaggtactt gattttttaa cattcttact ggattttttc ctgaaaatac
    23101 acctgctgct gtgaattgac atgccatata tacaaaatgt aaaggtatta ctactaagta
    23161 tactttccag aataccccta atattgtcca tacttcccct gctatagtta tatttgcaaa
    23221 agttcctgct atgtataaag gtaataaagg tataatcact gttgacaacg ttttcgttac
    23281 gattccttga aattcttgga agaaattaaa taaagtctct ccttttccat gatttcttaa
    23341 agctgatatt ccaagtccca ttacaaatgc aaatacgatt gcagctgtta catctaccat
    23401 aggctctaat ggaattgtga atattggagc taacattcct gcctctgggt ctccaatttg
    23461 acttgcaaca cttgcatcaa taaaatttgg gaagattaat gtagatactg caaatgataa
    23521 taatcctgct attaaggtag aaccatatgc tattcccgta gttatcccca ataactttcc
    23581 tgcacctgtt gctaaatcag ctatacctgc tgttacaaaa cctataatta taaatggtat
    23641 aacaaatttt aggaatgctg agaataaagt actaactgtt actaatattt gtactattat
    23701 ttctggtgca taacttccga ttaaaatacc gactatgata ccgatgatta gtttgggcac
    23761 taaacctatt tttttcttca taatatttac cctccataac ttttttaaat taagcattct
    23821 tgcacattga atacttagtt aaatattgga tatccactta ttatgataat agagttttac
    23881 ctctccgtca acaatttaac cattttttgt taataaaaaa aagaataaca tatataaatt
    23941 gttattcttc ttcatctttt atagaaaatt ctacattttc aattatattt gaatgtttat
    24001 ctttttttgc gataactcta tacttattaa tttcttcatt cactatacct atactccaaa
    24061 ttaaaactat agcatcatct ataaaaccaa atgcccctgc aatcatctct ggaattatat
    24121 caaccccaga tacaaagtag gatatagtaa aaattaatac agaaattacc tttattttat
    24181 caactatatt gattctttta tctgtcataa aatcaggtag tttaagtaaa tcaaatacaa
    24241 aaaccaaacc aaatttagca tttttaaatc tgaccaaaat tttttttaaa atatctaaaa
    24301 ctatattaat gattattttt aaaaactcca tatttatcac ctcttataat tataggttat
    24361 tctacattat aaccgatata attaatttga tgaacatatt agatataaat tttatcaatt
    24421 agtttatatt taaattataa taattcaatt cctgcactac tacaattttg aatcatatcc
    24481 tgtggccata ctccaacttg tacttctcct atatgagctt tttgtaagaa atacatacat
    24541 attcttgact gaccaattcc tccaccaatt gtatacggaa gttcgccatt taaaagcatc
    24601 ttatgatagt caaactcttt tctctcttca catccagcaa gtttcaattg tctatcaaga
    24661 gattcttcat caactctaat tcccattgaa gataattcta atgctgaatc taaaacagga
    24721 ttgtaaaata atatatcccc attcaattcc caatcatcat aatcagggct tcttccatca
    24781 tgtttttctc ctgatattaa tgtttttcct atttgagtta taaatactgc tttcttctct
    24841 tttacaattt tatcttctct ttcctttggt aataattctg gatacatatc ttctaactct
    24901 tgtgatgtta taaaagttat ttcttctggt aatatttctt ttataccttc atacatacta
    24961 catacaaaaa tttcagtatc tttaaatact ccatacaatc tttttacaac atcttttaga
    25021 gtcttttcat ttctactact tttatctata accaattccc aatcccattg gtctacataa
    25081 agagaatgta tattgtctaa ttcttcattt tttcttattg cattcatatc tgtatataaa
    25141 ccactaccaa cttcaaaccc atatttttta agggctaatc ttttccattt tgcaagtgat
    25201 tgaacaattt ctgcattttt attcataaat ggtatatcaa agcttacagc cttttctcca
    25261 tttaaattat catttgcacc tgtttctggc aatacaaata acggagaaga aactcttata
    25321 agatttaatt cattacttag tctcatttca aaaaaatctt ttaatttttt tatagctatt
    25381 tgtgtatcaa ttacgttcaa actactttta taatcctttg gtattattgt gctcatatca
    25441 ttcactccct atatttatat atttttccat taaaaaaacc cttcatccct atataaaagg
    25501 gacgaaaggt tgcttttccg cggtaccacc ctgattagtt tgaaataaat tcaaacccag
    25561 ctcacaatgt acagatatta ctcgatacac cccaatatga taacggtttg gttccgacta
    25621 agcctactct tttaaaattt cggttagcaa ctccaggatg ttcttcatta tagacttcat
    25681 attggtctca caccatcacc aactcgcttt aattacattc tataactact cttcctttca
    25741 aagtctttaa gagtttaatt caataaaatt tagctctctt atgttttcta tttattcata
    25801 atagtatgat aagtgattag ttaaaatttg tcaagtttat tattttttta tttttaaaca
    25861 ctttataaat ttttacctat actttataaa ttttcaaata tagtctttaa tctgtccata
    25921 cattctttta ataaagactg tggacaagct acatttattc tttcaaatcc tattccttct
    25981 tttccaaata tatacccttc atcaaataat acattagctt ttttaaacat taaatcctca
    26041 agttctactt catttaatcc ataagcagta aaatctaacc atgctagata tgttccttct
    26101 ggataaacaa gttttacttt tggtaggttt tcttttaagt attcatcaat aaattgtata
    26161 ttattatcta agtaatcatt taattcatta agccagtctt cactttcatt atatgctgct
    26221 attgtagcta caatagcaaa gggatttggt gaagttgcaa ctccaacagc tgtaacctct
    26281 tcttgatatt tcttttttag ttcatcatta ttcactatta tattagacaa ctgcattcct
    26341 gccagattaa atgttttact tggagctata catgtaacta ttttatcctt gtactctggg
    26401 cataatgact gtaaaggagt atgtttaaat ccttttctaa ttaaatcaga atgtatctca
    26461 tcagctatta tccataggtc atatttttta catatttcta caaccttttt taattcatct
    26521 tctctccaaa ctcttccaac agggttgtga ggactacaaa gtatcagtac tttattgttg
    26581 ctacacttag ctttttcttc taaatcatca taatccatct tataagtacc attctcatat
    26641 atcagaggat tgtttacaac gtccctatta ttgctcttta tcttagcttc aaatgggtaa
    26701 tacactggtt tttggactat tacaccttca ttagaattag ttaatattct tactaaaata
    26761 gcaacagcag gtacaacacc aggactaaaa ataatgtctt ttctatttat ttcccagtta
    26821 aatctccttt tataccaatc acaaactgca ttaaagtatt tatcactatc atttgaacta
    26881 tatccaaata ttttatgttc aactttttta cgtaatgctt ctattattgg ttctgcacat
    26941 tcaaaatcca tatcagcaat ccaaaaaggc aaaccatctt tttgtgcttt tgttgaacaa
    27001 ttagttctaa aatcccattt tacacaatct gttcctactc tatctattac tctgtcaaaa
    27061 ttatacttac tcattttttc cctcctaaga ataaaatttt tataattgtt aagtctatta
    27121 ctagatgcac tttggtatta ttttacaatt cttaatatta taataatatc acttttttgt
    27181 ccaatagatt taaaatataa ttacttttcc atcatatttt ttcaatattc caaaatcagt
    27241 ttcattatat caaaataata ttgctcatat taattatttt ctaattaaca taagcaatct
    27301 ttttctataa cttctataat tataatttta aatgtagaat tattagtttg gatgttatcc
    27361 caatctcttt tcaaccaatc tacaccatat atctacacca tattctaaag ctttcgtatc
    27421 aaatgttaca tttggattat gaaatcctgg tgtagcatca gctcctaatc caatatatgt
    27481 agttttacat tttagttttt gtgtataata atggaagtct tctccaccag aatttttaaa
    27541 tatacctata gatgcatcag ctcccaatac atcttttata gtttcttcag ccaagtttac
    27601 aagttcttca tcaaattctg ctgctggtac tccatctttg gaaattagta cagcttctgc
    27661 atttatagaa cttacagcag tttttacagc atgtattgct ttttctttta attcttctat
    27721 tatatcatta ctttctgccc ttatatctaa agccataaat gcttcatctg gtatattatt
    27781 ataagtttct cccttaactg atatatttgt tacttttata gaatttggta tagctggatt
    27841 tactcttatt gcatttattg cattcactgc taaacagaca gcatctactg catttactcc
    27901 taaatgtggt ttagaaccat gacaagtgat acctttaatc gtaaattcca ttatacagct
    27961 agcacaatgg tacattgcag gtatcgcctt tcccaaccta caatcttgtc caggtcttaa
    28021 atgcatccct accatttctt ctacatcatc caacaaacca gaatcaatta ctcttaatgc
    28081 tccatataat ttttcttcac ctggttgaaa aagtattttt aacttacctc tttttactaa
    28141 atttctctct tttattaact ttgcagctgt taaaagcata gaagtatgac catcatgacc
    28201 acaagcatgt atcattttct tttctccatc tatgataaat tccaatgcat ccatatctgc
    28261 tcttatagca aatactggtc cttctttttt agaatctata gttactataa taccagttcc
    28321 tgcaactcct ttattaatat tttcaaaccc cattttttca agctctttta aaacaaactc
    28381 tgatgtctta tattcttcaa atcctttttc tggtatagtg tgtagataat catacacttt
    28441 atgtacatac tcaatataat ctttgttagt tcccataatc tagctcctcc ttaaaattta
    28501 aactttccat ttatattaaa tttttattat aattacctat actacgaaat aattcattat
    28561 aagcattgca atacaaccat ttaaagttgc tataacaaac atatgtggat aatatttagt
    28621 ttttaactca gctgttccta aaagtctccc cataaattgt atctgtgctc ctgaaagcaa
    28681 taacattggc aatagaattg caacatgtgc ttgacttatt tgacctgctg taaataaacc
    28741 tgcgattact ccagatgctc ctcccattga tataaatatt gtcattatag ctgttgctgc
    28801 aacacctgga agtccaaaga tactcattac aggtgtaaat attgtcccta ttacatccaa
    28861 aagacctgtt aaattgaaca cagaaattat agcaaaaccg aagatgacat ttggagccat
    28921 tgaatttgta cttatttgga atccatttct agctcctcca ataaacgcat ctatcacatt
    28981 tcctttttta acttttactt ctttttttgc tacctcactc atgttaagct tcccccttgt
    29041 caaatttatt taaatataat ctaagtaagt tcgccccaac aaacttcata attaatatta
    29101 aagcaagtgg tataaatatt ggtacatctc ctataaatgg aaatagtgct gctccagaag
    29161 caaagaaatt cgtaattgca gacgcacctg aaaattgaaa tgcacaaaat acagcttttt
    29221 gtttttcatc tatataccca tcatctttaa gttctttagt catagatgca gctgcatctg
    29281 tagactgtaa actagctatc aaagttagtc ctgcaaatcc aggtaaccct aataatggct
    29341 ttaatagtgg actaagaagc ttttgagctg ccttcattcc atctaagtgg tctataactc
    29401 taactactcc aagtgcaaac ataactgatg gtattaatgt caatgcatat agccatccat
    29461 ctcgagggcc agtacctcca tcacctctaa agtttgcagc taatgtacct acaccatcat
    29521 taattgttcc caatgttcca aaagaaccta atacattgtt aaaatccaat acttttaatg
    29581 gtcctggtgc atctttaaat attcctgaaa aaaataatgc tcccaatatt aatgctacat
    29641 acccatacca ttttacctca actttttctg ttgttgattc taatttactc atataaattc
    29701 tccctcctgt ttaaatgaat tttattagta tacttgttta ataagcaaaa cttatgccaa
    29761 taccttaatg ataaagcctt tgtatttatt tttttattgt aaatttattc cagcgattaa
    29821 atagcttata attacttact tttcaagttt aaaaatataa tat
//
LOCUS       DAEMHZ010000040        29446 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000040 DAEMHZ010000000
VERSION     DAEMHZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.040"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            74..229
                     /locus_tag="KPK72_001646"
     CDS             74..229
                     /locus_tag="KPK72_001646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488874.1"
                     /translation="MDEERKKLIDDAEKLANIGINQILKFRDSLDIENIKYINIVATN
                     LKPNIKK"
     gene            complement(248..604)
                     /locus_tag="KPK72_001647"
     CDS             complement(248..604)
                     /locus_tag="KPK72_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488875.1"
                     /translation="MHERIYKIRTSLKMSQREFGEKLGVSRDVISNIEYNRVTPKPVF
                     IQHLCELYNVNKEWLLTGEGEMFIEFSENEKVLKIATDIINEDDKFMKNILFTFSNLN
                     DEQKEFLINLMKNMTQ"
     gene            767..952
                     /locus_tag="KPK72_001648"
     CDS             767..952
                     /locus_tag="KPK72_001648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488876.1"
                     /translation="MKTQKTTSHLNNLNLDDDDRKLIQMTLLFKKLNLEQQNYISAMA
                     DGMKVSNELLEKSTMNN"
     gene            967..1725
                     /locus_tag="KPK72_001649"
     CDS             967..1725
                     /locus_tag="KPK72_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG2488877.1"
                     /translation="MSNNLMVFEGKEVEVFEFEGQILFNPKHVAECLDIKNVNENLRN
                     MNNKQVIKLTNSKISSTDFRKLHNTGENFLTESGVYKLIFKSRKEEAERFQDWVTDEV
                     LPSIRKTGTYSTKSKDESEIKYMNAQARLKNARAREAKIYLELADKVSQAGIPEFAQI
                     MYSKTTELISGEALIPLPKREKKTYSATEIGKILGISANKVGGLANAYNLKTDEYGIK
                     VWDKAKYSDKQVPNFRYYENVIPVLEKALNAFEN"
     gene            complement(1889..2233)
                     /locus_tag="KPK72_001650"
     CDS             complement(1889..2233)
                     /locus_tag="KPK72_001650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488878.1"
                     /translation="MTIEEKLKQLILDRYKSIRAFTKEIDTPYSTIDTMLKRGVGGTS
                     ITTVLKVCNALNIDADALIENELREKQNSINISIKSSEQDILKKYNALDEHGKDIVTT
                     ILEKEYIRVIKK"
     gene            2459..2629
                     /locus_tag="KPK72_001651"
                     /pseudo
     CDS             2459..2629
                     /locus_tag="KPK72_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            2635..3438
                     /locus_tag="KPK72_001652"
     CDS             2635..3438
                     /locus_tag="KPK72_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG2488879.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETEIRNISNNFNNNKERFIE
                     GKHYFCLQGDDLRTFKRDSYDIGIAPNVNKLYLWTERGANRHCKILDTDKAWEQFDNL
                     EESYFNKKKDTLITNQLSPELQLFKQIFDTVAKQEIEQKQIKQDITETKQEIKSIKDV
                     VTLNTTDWREETRKLIVSMAQNLGGNEYINTLRKESYELLCKRFNVDLKRRLNNRRSK
                     MAEKGVCKSRREKFNYLDVIQEQNYLIEGYVIVIKDMAIKYGVFMEKTN"
     gene            3410..4114
                     /locus_tag="KPK72_001653"
     CDS             3410..4114
                     /locus_tag="KPK72_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488880.1"
                     /translation="MEFLWKKLINIINKIKQYRRNKKMKENNRIVSAINEDIVGVTVD
                     GEIDLTDKELEMVNNFKKVMNSINNKIGIDCGISLSDKDIENNKNILDKIASKVAWHY
                     KKMNFEHLEMDEKLFDDFTFEFMLKFFGDMEEYELIKYLTYIGEESNELSDRERIFYK
                     IVDTLDTIADIKISDQNKIEKEVGMCIGEDNYITYCIDEDLIKFYIKDEEELVINKNS
                     PLLYMLDTLFYEVHEE"
     gene            4150..4401
                     /locus_tag="KPK72_001654"
     CDS             4150..4401
                     /locus_tag="KPK72_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488881.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDEEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWKEFDEVAKEVAINIAMKKAETVES"
     gene            4516..4677
                     /locus_tag="KPK72_001655"
     CDS             4516..4677
                     /locus_tag="KPK72_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488882.1"
                     /translation="MLSLDISKKNVMTLKKDGKFLADIVFKDIKTGKKISVGTLNKKS
                     AGQSNQHNK"
     gene            4722..4895
                     /locus_tag="KPK72_001656"
     CDS             4722..4895
                     /locus_tag="KPK72_001656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488883.1"
                     /translation="MVEVNIKIKGEASEIIDSIRAIFNSFGEDSMTEIKAEQEYKSRR
                     SRKLKTEDLSDQE"
     gene            4986..6164
                     /locus_tag="KPK72_001657"
     CDS             4986..6164
                     /locus_tag="KPK72_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2488884.1"
                     /translation="MGLGKTFVGSEKLINLGSNTNLLICQKSKIDDWVHHFKTYYPDM
                     GIINGTKKLSEGIEYFKIVEVPFIVVINYELAFRRKELLDLKDFTLMLDESSMIQNEK
                     AKRSKFVLSLNPKNVILLSGTPCSGKYEQLYSQIKLLGWNISKDLYWKQYIDVEYKDI
                     GGFPMKVVTGYKNVDRLKKKLRDYGAVFMMSDEVFDLPKQIDNVMKISTTREYRKFKK
                     DSIISISDDIELVGDTTLTKMLYERQLCSQYNKFKLEAFGDLINSTNDRLIVFYNFNE
                     ELDRLIELSRDRPISIVNGSIKDLSNYEEYNNSITFVQYQAGAMGLNLQKCNKIVYFS
                     LTLSCELFMQSKKRVHRIGQENTCFYYYMICRNSVEENIYKSLQSGVDYTNNLFERGE
                     "
     gene            6168..6470
                     /locus_tag="KPK72_001658"
     CDS             6168..6470
                     /locus_tag="KPK72_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBG2488885.1"
                     /translation="MGAEKQFENKVKKFLKEQGCWFIKYWGGSAYTKSGIPDLLVCCS
                     GKFIGIEVKGEKGKASELQKYNIKEIEKAGGIGIILYPKDFDRFKKMILEIKKEGE"
     gene            6471..8156
                     /locus_tag="KPK72_001659"
     CDS             6471..8156
                     /locus_tag="KPK72_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2488886.1"
                     /translation="MQFSHSRIGVFKSCPYKYKLQYIDKVKTILNADSNNALILGTSL
                     HTGIEKDIDAAVKEYYFSYPSITDLHINEVMKLDYLIPKVKEILPKGFNEVQITTSDF
                     IGFIDLLAPHVYMTDTNLNTNETTIYYEDEERVFDIYDFKYSNNIDRYLESEQLHLYK
                     YFYEKQHTNHEIKDLYYVFVPKIQIRQKKTENLHQFRKRLLNELDKSEIKIVKVEYDP
                     NKVINFLVDIKRCLENKEFEKNQNKLCNWCDYQEYCEKGIDYMLLPNSERRSVEGINK
                     KTLWIYGAPFSGKTTLANDFEKPLMLNTDGNIKFVDAPYISIKDIVTVEGRMTKRQLA
                     WDVFKEAIRELEKKENDFETIIVDLLEDTYEACRLYMYDKMGITHESDDSFRAWDKVR
                     TEFLSTIKRLMNLDYKNIILISHEDTSKDITKKGGDKITAIKPNLQEKAANKIAGMVD
                     IVARVVADGDVRTLSFKTNEVIFGGGRLKVKSNEIPLDYNELMKVYDDISIEKKDVKK
                     SSKTKKSNAEEDVEMIPIVEETSEKTEKEQTDEEVIEQEEAIKPVKKTRKKRS"
     gene            8208..8651
                     /locus_tag="KPK72_001660"
     CDS             8208..8651
                     /locus_tag="KPK72_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBG2488887.1"
                     /translation="MDFSKFDKAIDVKGLKEDIKEASENSGKFKDVPHGTYEVEINKM
                     ELSESKKGDPMFVCWFKILEGDYKDSLIFMNQVVKQGFQIHIVNEFLRSLETDIEVEF
                     ESYSQYAQLIMDIAEEIDGELEFAIEYGEKKGFNTFTIKDIFEVA"
     gene            8865..10598
                     /locus_tag="KPK72_001661"
     CDS             8865..10598
                     /locus_tag="KPK72_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488888.1"
                     /translation="MIFYDFEVFSYDWLVVFIDVLNKKEEVIVNDIDKLNSFYIEHRE
                     DIFIGYNSRHYDQYIFKGLLCGFNAKEINDYIIVKGQPGWKFSNLLRNIQINNYDVMT
                     SFHGLKQLEGFQGHSIKESNVSFNIDRPLTNDEIEETIKYCRYDVEQTIDVFIERKSE
                     FEAHMGLIKAFKLPVAYIGKTQTQLTAIILEATKKEHDDEFDLQIPDTLKIEKYKEVL
                     SWYKNPLNHDYSKNLKINISNVPHVFAWGGVHGAITKYYGEGYFLHVDVNSFYPSLMI
                     RYNYHSRNIKNPQKYVEIYDKNLQLKKEKSPLRPAYKLAVNKTYGGMKDKNNNLYDPR
                     QANNVCVSGQLLLLDLIEKLEGHCKLIQSNTDGLIVKLNTIDDYELIDDICYEWEQRT
                     GMGLGFDVYTKIFQKDVNNYLVITEDGEVEAKGLYIKDLGNLDYDLPIINKALKNYMV
                     NNTPIEETINNCNDLIEFQKIVKISSKYSHGLYSPTIEGKTKKVFIGGKILNDKCFRV
                     FASKNKNDGGIYKVKNKEKNPEKFANTPDKCFIENGSVIGKKVPRKLDKIWYIDLANE
                     RLKQFGVLLSN"
     gene            10610..12493
                     /locus_tag="KPK72_001662"
     CDS             10610..12493
                     /locus_tag="KPK72_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HBG2488889.1"
                     /translation="MSFDLFKGYIVTNNKKAAEKFKNVKKLKTYEQIKNLPEFAGVLA
                     EETVLVDIDDFESSEILYKIVQDLKLKCRVYKTTRGKHFLFKNTSLEKNRTKCRLAIG
                     LNSDIKLGCKNSYSILKFNNIEREILYDSKEIQEMPMHLTPIKNGIDFLSLGEGDGRN
                     QALYNYILTLQSNDFTVEEIRETIRVINKYVLKTPLQDNELEVILRDESFQKKIFFNS
                     KGSFLFDEFAKYIKNNNHVIKINDQLHLYKDGIYVDGQARIEAEMINNISNLNKAKRS
                     EVLSYLNLLISENTSMSEANLIAFKNGIYNIVDDSFIEFSPEFIITNKVNWNYNPVVY
                     SKLVDKTMNKLSCGDFEIRMLLEEVVGYCFYRRNELRKAFILTGDKANGKSTYLDMIK
                     TLLGDENTSALDLKELSDRFKTAELFGKLANIGDDIGDEFIANPAIFKKLVSGDRVNV
                     ERKGQNPFDFNNYSKFLFSANNIPRIKDKTGAVLDRLIIIPFNASFSVNDKDFDPYIK
                     YKLREQEAIEYLINLGLQGLKRVLQNRKFTVPDKVKKEILEYEEVNNPILGFFKEVDK
                     IENESTKEIYRKYQEYCILNGLQPISNIEFSRQVVKKFGYEVKDKRIQGKKYKVFTRA
                     VPG"
     gene            12839..13267
                     /locus_tag="KPK72_001663"
     CDS             12839..13267
                     /locus_tag="KPK72_001663"
                     /inference="COORDINATES: protein motif:HMM:TIGR01636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2488890.1"
                     /translation="MQGCENGVTDYEKLEELLNSYSNLDIEIREIELKVRGSSLRGIE
                     LNGMPKGNNVSSPIENELIYVERLENEKIYLQIKKESINNMLNLLDDFERNLIELRYF
                     KKLQYKQISYELNISEVYVGFKRKKALNKILPFAKKYNLI"
     gene            13441..13869
                     /locus_tag="KPK72_001664"
     CDS             13441..13869
                     /locus_tag="KPK72_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBG2488891.1"
                     /translation="MAKLTEKQKRFCDYYIETGNATEAAIRAGYSEKTAKVIGAENLT
                     KPYLKSYIDEKLKEFESNRIADAKEVMEYLTKIVRNEAKEEVVVVSEYGPEIIKKDVS
                     IRDRNKAAELLGKRYRLFTDKVEVEGAIPIVITGDDEIED"
     gene            13850..15100
                     /locus_tag="KPK72_001665"
     CDS             13850..15100
                     /locus_tag="KPK72_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2488892.1"
                     /translation="MMKLKIKKINIAKLVGKGYKDYWRFKGRYRVCKGSRASKKSKTT
                     ALYYITKLMQHPEANLLVVRKVFGTLRDSCYKELKWAIHQLGVDNYWDSTTNPLEITY
                     IPTGQKIYFRGFDDPLKITSITVDVGVLCWCWIEEAYEITDENSFNMLDESIRGKVPS
                     GLFKQITMTFNPWNEHHWIKKRFFDTKDKDILSKTTNYLCNEFLDDADKKVFETMKKN
                     NPRRYKVAGLGDWGIVDGLVYENWEERVFNIDTLIEQNIKSAFGMDFGYTNDPTTLFC
                     GLVNEPSKEIYVFDEIYKHGMSNERLYREVSRKGYSKEHITADSASPKDIDHLRELGL
                     RNIKGSRKGKDSVNNGIQYIQDYKIIIHPKCVNFLTEISNYTWDKDKFGNKINKPIDD
                     FNHLMDAMRYALEDFIKGEVFSFD"
     gene            15135..16568
                     /locus_tag="KPK72_001666"
     CDS             15135..16568
                     /locus_tag="KPK72_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007440343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2488893.1"
                     /translation="MLNKPLKQSANNLKYIEYEINKWKTSNSRKEQLQGDRYYKGEHD
                     ILRRKRTVIGEGGKLTEVENLPNNKIVDNKYANLVDQKVNYLLGKPLTFQTENEIYNS
                     LLKDIFNKKFLRTFKNLCEDSLNGGISWLHPYYSEDGKLSFKRFPSYEVLPFWKDSEH
                     TILDFAVRLYEVKAYSGANEKIIEKVEVYSKEGIKRYILQGMTLIADSESPYSSYLVV
                     ETDDIHEELNWDRIPLIPFKYNNKEIPLIKRVKSLQDGINIMLSDFENNMQEDARNTI
                     LVLQNYDGQNLGEFRKNLAQYGAVKVRTVDGAVGDLKTLEIKVNSDNYKSILEVFKKA
                     LIENGRGYDAKDDRMSGNPNQMNIQSMYSDIDLDANGMETEFQASFEELLWFVNMHLL
                     NTGQGNYENEEVEVIFNRDILINETESISNCQNSVGLLSDETIISQHPWTIDVRQELE
                     KKKKQEEAQREYDDLIPNNQDGVIDET"
     gene            16558..17955
                     /locus_tag="KPK72_001667"
     CDS             16558..17955
                     /locus_tag="KPK72_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG2488894.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNKINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKKLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIVATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPWFEDEEGYRAAKGKDGKTYYVPSSMKYN
                     EWYEKYVKNNDISEIKNSAIIDSIKEDIKNGNYNLNIHDGKQGKHLKGHNNYIEGRSY
                     LTITKEEAQELVNRHAGNGIIKFNRSGEWDKKELIEIDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            17970..18170
                     /locus_tag="KPK72_001668"
     CDS             17970..18170
                     /locus_tag="KPK72_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488895.1"
                     /translation="MKLWEYVGKNIQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENISKK"
     gene            18236..18772
                     /locus_tag="KPK72_001669"
     CDS             18236..18772
                     /locus_tag="KPK72_001669"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2488896.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTVTKEEYTNL
                     ETQLNTANETIEGFKGVMTKQEVEQLKATYEAEIKQIRRDSIDERLLTENRTRNKKAT
                     KALLEDIEEEDYEKYESLRLAQIKKLQQSEESKFLFEEVQETKVNFRGVNPGESNPIA
                     EKSTEDFTYEDWCNQLEN"
     gene            18792..19862
                     /locus_tag="KPK72_001670"
     CDS             18792..19862
                     /locus_tag="KPK72_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage coat protein"
                     /protein_id="HBG2488897.1"
                     /translation="MANTKFDAKSFNAEAFGKYMESAPTLKRNELLKSRALKSNSEIR
                     NLFSSQTTTSYGRIPMYGNLEGDALNYDGMTDITADTTVTYERGVVVVGRAKAWMEKD
                     FSQDITAGVDFMANVRNKVASFWEEIDQDTLLSILKGIFSMTGTENKKFVDAHTSDIT
                     KNGDGKVSAESLNTTIQKACGDNKNKFSLAIMHSSVATNLENLNLLTYLKYTDEQGMQ
                     RDLGLATWNGRTVLIDDSMPVEDVAESASGAGDGYTKYTTYILGDGAFDYEDIGAKVP
                     YEMDRDPKTNGGQDTLYTRQRKVFAPCGISYEKKKQVSLSPTKSELEDGSNWTLVNDG
                     ATTKTKYIDHKSIAIAQIISKG"
     gene            19868..20269
                     /locus_tag="KPK72_001671"
     CDS             19868..20269
                     /locus_tag="KPK72_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488898.1"
                     /translation="MENNIIDDIEKRLESFEYTLKDGDKWLIGFVKEKIENIIKLDCN
                     INEIPKELKNIEIDMIVGEFLFTKKNMGQLDIESLNFEVVEKSISEGDTKIDFAIGSG
                     SQTPEQRFDTFVNYLLTYGKNKILTFRCLRW"
     gene            20263..20610
                     /locus_tag="KPK72_001672"
     CDS             20263..20610
                     /locus_tag="KPK72_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488899.1"
                     /translation="MVNRTRKAIEMLYRDKCTIVEYQPIKDPITKRTNNKEVVVLENQ
                     PCKLSYKNITSATDGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            20664..21035
                     /locus_tag="KPK72_001673"
     CDS             20664..21035
                     /locus_tag="KPK72_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2488900.1"
                     /translation="MEKLTKIDLDKFCKDAARELAARLLGKVIRRTPVDTGFLRQGWN
                     GVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDGKGWVKGQHFLTISEMELQ
                     SQVDKIIEKKLLILLKGVFDA"
     gene            21028..21465
                     /locus_tag="KPK72_001674"
     CDS             21028..21465
                     /locus_tag="KPK72_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488901.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            21458..21634
                     /locus_tag="KPK72_001675"
     CDS             21458..21634
                     /locus_tag="KPK72_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488902.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNKKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            21635..22945
                     /locus_tag="KPK72_001676"
     CDS             21635..22945
                     /locus_tag="KPK72_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG2488903.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTRRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            22963..23433
                     /locus_tag="KPK72_001677"
     CDS             22963..23433
                     /locus_tag="KPK72_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2488904.1"
                     /translation="MFQQIKARDTISASKAECYVTIEGKRYNFMQAINLEAKMEKNKS
                     EVPILGSTTKGNKSTGSKYTGSATFHYNTSIFRELLYKYKETGEDIYFDIQVTNEDPT
                     SSAGRQTIILKDCNMDSGIIVKFDADGEYLDEDMDFTCEDWELVEKFNIINGME"
     gene            23505..23945
                     /locus_tag="KPK72_001678"
     CDS             23505..23945
                     /locus_tag="KPK72_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2488905.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYIVSNRFLDKEGKPVEWELRVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            24207..25001
                     /locus_tag="KPK72_001679"
     CDS             24207..25001
                     /locus_tag="KPK72_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG2488906.1"
                     /translation="MGLFSKKNKKPCCICGSEKGLMPSIEGENFCTACNCKYIDFSEN
                     ILKVTSIMKMMANLEGMKKFIEVEKKNLKLLEKFTETKSINSSISFDEEQNLLKISYK
                     NRNQILVEKIIKFDDILEFELLEDGETIVKGGLGRAITGGVLFGGTGAVVGGITGKKT
                     SRKVVEILKIKITVKDINNPIEYINLINSKLKTNSSIYQKALSDAQEILSILSIITKY
                     NVMEDKKEESMCNSTADEILKYKNLLDMEAITQEEFDAKKKELLNL"
     gene            25071..27413
                     /locus_tag="KPK72_001680"
     CDS             25071..27413
                     /locus_tag="KPK72_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG2488907.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIREADGQQRRLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLSMINDGQQSDSGLSKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGSAFSNTREIVSFAEQLNKKFVIAGASTQEMSSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAASAETNKQFE
                     QMPYTLGQIFTSVKNNAVMVFGAIQKKIEDTVSSRGFRTFITDVKDSLYVLGAVGFNV
                     FSGFINLLSSPAFQNFFNVMIVGTSLVVQGLGWIITQALSVANIFSRNWSIISPIILG
                     VVSAISTYIIALGIMQAVTIAGTVANWMSWTSEVIRQRGILGTARALAIGKAEQWGFN
                     AAIYACPIFWIVLGIIAVISAVFILVAVYNHFAGTNISVISLAVGAWYWLCAVVYNII
                     AGIVNAINICVVEIVNIFRTGLYAVQCFFIDMANAGLKAGVNLDKAFDKFATNLANGI
                     IKAVNIAVKGLNWLVQQINKIPGLDLPQMKEFQKVNTVIGTKTTFKPIQKPPEPKAWK
                     TELVEYKNLKSEFIKGYDVGHQLQNKLKDTFDISKIAEDAKKKLGLDDLWDKKYGLGD
                     SIGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVN
                     IKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGIV"
     gene            27425..28126
                     /locus_tag="KPK72_001681"
     CDS             27425..28126
                     /locus_tag="KPK72_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2488908.1"
                     /translation="MAYDFYLDGVQLPITPGKLEVKITNKNKTVDLINVGEVNILKTP
                     GLSEFSFEAEFTHNKLPFYRGQFKDVQFFLSKLELLKTDCKPFQFIVSRQLGNKVLFN
                     TNRKVSLEEYNIVEDAENGSDFKVAIKLKQYRDYSTKKLVLATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNAKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKSMMDKANKGKK
                     VPKYTIYKGQVLKLG"
     gene            28119..>29446
                     /locus_tag="KPK72_001682"
     CDS             28119..>29446
                     /locus_tag="KPK72_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2488909.1"
                     /translation="MADDLVLANDRDIRLVIAHWEDFYEPVVLDNITWEIERRGSPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRLTLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TSKRDVYIAQDSAHMRSWGILQMFETVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLS"
BASE COUNT        12172 a         3022 c         5475 g         8777 t
ORIGIN      
        1 atttgaagta tttttaagtg tatatttata atataagggg aatttttata tttatgaatg
       61 gaggaatata gtaatggatg aagaaagaaa aaaattaatt gatgatgcag aaaagctagc
      121 aaacatagga ataaatcaaa ttttaaagtt tagagattca ttggatatag aaaatataaa
      181 atacataaat atagttgcaa caaatttaaa gccaaacata aaaaaatagc cacccttttg
      241 ggtggtttta ttgtgtcata tttttcatta agtttataag aaattctttt tgctcatcat
      301 ttagattact aaaagtaaat aatatatttt tcataaattt atcatcttca tttataatat
      361 ctgttgctat ttttagtacc ttttcatttt cagaaaactc tataaacatt tcaccttcgc
      421 cagttaagag ccattcttta ttaacattat atagctcaca taagtgttgt ataaatacag
      481 gcttaggtgt aaccctgtta tattcaatat tacttatgac atctctgctt acaccaagtt
      541 tttcaccgaa ttcacgttga gacattttta aggatgttct tattttatat atacgttcat
      601 gcatgatttt cctcctaata attacgttat taattagata gtataacaca aaaaagaatt
      661 agtcaacata aatgttgtgg taaaaacaca aaaatattta aaaacaagtg ttgactacac
      721 aaaaataaga tgatatactg tgtttataac acaaggaggt gctaaaatga aaacacaaaa
      781 aactacttct catttaaata atttaaattt agatgatgat gatagaaagc ttattcaaat
      841 gactcttttg tttaaaaaat taaatttaga acaacaaaat tatatctctg caatggctga
      901 tggaatgaaa gtgtcaaatg agcttttaga aaaatcaact atgaataatt aagggggaaa
      961 gaatttatga gtaataactt aatggtattt gaaggaaaag aagtagaagt gtttgagttt
     1021 gaaggtcaga ttttatttaa cccaaaacat gttgcagagt gcttagatat aaaaaatgta
     1081 aatgaaaatt tgagaaatat gaataataaa caagtaatta agttaactaa ttctaaaatc
     1141 agtagtactg actttagaaa actacacaat acaggagaaa attttcttac tgaaagtggt
     1201 gtgtataagt taatctttaa atcaagaaaa gaagaagcag agagatttca agattgggtt
     1261 actgatgaag ttttaccatc gataagaaaa acaggtacat acagcactaa atctaaagat
     1321 gaatcagaaa taaaatacat gaatgctcaa gcaagattaa aaaatgcaag agcaagagaa
     1381 gcaaagatat atttagagtt agctgataaa gtttcacaag ctggtatacc tgaatttgct
     1441 caaattatgt attcaaaaac tactgaactt atttctggag aagcattaat acctttacct
     1501 aagagggaga aaaaaactta ttcagctaca gagataggta agatattagg aatttcagct
     1561 aataaagttg gaggattagc aaatgcttat aatctgaaaa cagatgaata tggaattaag
     1621 gtatgggata aagctaaata ttcagataaa caagtaccta attttagata ttatgagaat
     1681 gtaataccag ttttagaaaa agctttaaat gcttttgaaa actagcactt tgaaaactaa
     1741 atacagaata ttcttgaaat ggtggtgatt taaagattat atgaatttag cgggtaaaaa
     1801 aacataacaa attcaaggga aaaattttat ttatatcgaa cttatgtttt atataaatga
     1861 aaaaatagaa gccctaatta taggacttct atttttttat aactcttata tattcctttt
     1921 ctaatatagt agttactatg tctttgccat gttcatctaa agcattatat tttttcaaaa
     1981 tatcttgttc agaggatttt atagatatat ttattgaatt ttgtttttcc cttaattcat
     2041 tttcaatgag tgcatcagca tcaatgttta aagcattaca aacttttaga actgttgtta
     2101 tgctagtacc tccaacacca cgtttaagca tagtatctat cgttgaataa ggagtatcaa
     2161 tttcttttgt aaaagccctg atgcttttat acctatcaag aattaattgt tttaattttt
     2221 cttcgatagt cattttatca ccacctttac attaaatata aacgtaaaat cacaaaaatg
     2281 caacagtaat ttacgaaatt acaaaaataa aatgcaaaaa tgtattgact attcactaaa
     2341 ttgagtgtat tattaaaata taacttacga aatttagtga ataagtttag aaaggagaat
     2401 atgtatgttt aaaaatttaa gagctgaaat ggtaagagca aatataacta atgaaacatt
     2461 agcaaaagca ttgaatctaa atgtaagtac agtgtctgca aaattaagaa atgctaacag
     2521 gttaaagtac atagaagcaa aaaatataca attaatgttt ttcccgaatc taagtactga
     2581 atatcttttt gaaattgaag atgaagttaa agaaaattat agggggtaac aaacatgaat
     2641 aacttacaag taatagaaag aaataatgag agagttctaa ctacacaaca attagcagat
     2701 gtatatgaaa ctgaaattag aaatataagt aacaatttca ataataataa agagagattt
     2761 attgaaggta aacattattt ttgtctgcaa ggtgatgatt taaggacatt taaacgcgat
     2821 tcatatgata taggaattgc acctaatgta aacaaacttt atctttggac tgaaagagga
     2881 gcaaacagac actgcaaaat attggatact gacaaagctt gggagcagtt tgataactta
     2941 gaagagtctt actttaacaa aaagaaagat accttaatta caaatcaatt aagtccagaa
     3001 ctacaactgt ttaagcaaat atttgatact gtagcaaaac aagaaataga gcaaaaacaa
     3061 ataaaacaag atataacaga aactaaacaa gaaataaaaa gcataaaaga tgttgttaca
     3121 ttgaatacaa ctgattggag agaggaaaca cgcaagttga tagtatcaat ggcacaaaat
     3181 ttaggtggaa atgaatatat aaatactctt agaaaagaaa gttatgaact tctttgtaaa
     3241 agatttaatg ttgacttgaa gcgaagatta aataatagaa gaagtaaaat ggcagaaaaa
     3301 ggtgtatgca agtctagaag ggaaaaattt aattacctgg atgttataca agaacaaaat
     3361 tatttaatag aaggttatgt aattgtaata aaagatatgg ctattaaata tggagttttt
     3421 atggaaaaaa ctaattaata ttattaataa aattaaacaa tataggagga ataagaaaat
     3481 gaaagaaaat aatagaatag tgagtgctat aaatgaggat atagtaggtg ttacagttga
     3541 tggagagata gacttaacag ataaggaatt agaaatggta aacaatttta agaaagttat
     3601 gaatagcatt aacaataaaa taggtattga ttgtgggatt tcacttagtg ataaagatat
     3661 agaaaataat aaaaacatat tagataagat tgcatcaaaa gtagcgtggc attataagaa
     3721 aatgaatttt gaacacttag agatggatga aaaactcttt gatgatttta cttttgagtt
     3781 tatgttaaag ttctttggtg atatggaaga atatgaatta ataaaatatc ttacttatat
     3841 aggagaagaa agtaacgaat tgagtgatag agaaaggata ttttataaaa tagtagatac
     3901 acttgataca atagctgata ttaaaatatc agaccaaaat aagatagaaa aagaagttgg
     3961 tatgtgtata ggtgaagata attatataac ttattgtata gatgaagact taataaaatt
     4021 ttacattaaa gatgaagaag aactagttat aaataaaaat agccctctgc tatatatgtt
     4081 agacacacta ttttatgaag tacatgaaga gtaaaaattg caataattaa ataagtataa
     4141 gacaagttta tgagctcata ttttataaca aggggtgatg ttatgaaaat actacaacaa
     4201 tttaaaccat acaaatatga tgaagagaaa aacatagaat atgatgaaaa aattatagct
     4261 aatcaaaaca cagaagttta tatagtatat ccacgtatat ctgatgaaga gaataggaga
     4321 agatggaaag agtttgatga agtagccaaa gaagtagcta ttaatatagc aatgaagaaa
     4381 gctgagacag tagaaagtta agaaagtaga gaagttgcta atacttagca ctttctcaaa
     4441 gaagaaaatt ggacaactat ttttaaacaa ctaggaataa ataagcgata gtatgtttga
     4501 aagaaggtga tgaaattgct tagtcttgat attagtaaga aaaatgtgat gactcttaaa
     4561 aaagatggta agtttttagc agatatagta tttaaggaca ttaaaactgg taaaaaaata
     4621 tcagttggaa cattaaataa aaaaagtgct ggtcaaagta accaacacaa taaataaaaa
     4681 aaaacatatg aaaattataa cataaaaagg agagatttaa aatggttgaa gttaatataa
     4741 aaattaaagg cgaagcaagt gaaataattg actcaattag agctatattt aatagctttg
     4801 gtgaagattc aatgacagag ataaaagcag agcaagaata taaaagtaga cgtagtagaa
     4861 agttaaaaac agaagattta tcagaccagg aataaagaag gtgatataag ttgcaactat
     4921 ataagcatca agaggaagct ttaaagctaa cagaatataa taataaatgt gcatactacc
     4981 ttgacatggg gcttggtaag acatttgtag gaagtgagaa attaataaat ctaggaagta
     5041 atacaaattt attaatctgt cagaaatcaa aaattgacga ttgggtacat cactttaaaa
     5101 catattatcc agatatgggg ataatcaatg gtacaaagaa acttagtgaa ggcatagagt
     5161 attttaagat tgttgaagtt ccatttatag tagtaattaa ttatgaatta gcttttagaa
     5221 gaaaagagtt attagattta aaagatttta ctttaatgtt agatgaatca tcaatgattc
     5281 aaaatgaaaa agctaaaaga agtaagtttg tactaagttt aaaccctaaa aatgtgatac
     5341 tactttctgg tactccatgt agtggtaaat atgagcaact atattctcaa attaagctac
     5401 tagggtggaa tatatcaaaa gatttatatt ggaagcaata tattgatgtt gagtacaaag
     5461 atattggtgg ttttccaatg aaggtcgtaa caggttataa gaatgttgac agattaaaaa
     5521 agaagttaag agattatgga gcagtattta tgatgtcaga tgaagtattt gacttaccaa
     5581 aacagataga taatgtaatg aaaattagta ctacaagaga gtataggaag tttaaaaaag
     5641 attcaattat aagtatttca gatgatattg aacttgtggg agatactaca ctaactaaaa
     5701 tgttatatga aagacaatta tgtagtcaat acaataaatt taaattagaa gcttttggag
     5761 acttaataaa ctcaacaaat gacagattaa tagtatttta caattttaac gaagagttag
     5821 acagattaat agaattatca agagatagac caatatcaat agtaaatggt tctataaaag
     5881 atttaagcaa ttatgaggaa tataataact caattacatt tgtacaatat caagcaggag
     5941 ctatgggttt aaatcttcaa aagtgtaata agattgtata ttttagtctt actttatctt
     6001 gtgaattgtt tatgcaaagt aaaaagagag ttcatagaat aggtcaagaa aacacatgtt
     6061 tttattatta tatgatttgt aggaatagtg ttgaagaaaa tatatataaa agcttacaaa
     6121 gtggtgttga ctatacaaat aatttgtttg agagaggaga gtaatccttg ggtgcagaaa
     6181 aacaatttga aaataaagtt aaaaagtttt taaaagaaca aggttgttgg tttattaaat
     6241 attggggagg ttcagcatat acaaaaagtg gtataccgga tttattagtt tgttgtagtg
     6301 gtaaatttat aggtattgaa gtcaaggggg aaaaaggaaa agcaagcgaa ctacaaaagt
     6361 ataatattaa agaaattgaa aaggcaggtg gaataggaat tattctatac cctaaagact
     6421 ttgatagatt taaaaagatg attttagaaa taaagaaaga aggtgagtaa atgcagttct
     6481 cacatagtag gataggagta tttaagagtt gcccatataa gtataagcta cagtatattg
     6541 acaaggtgaa aacaatatta aatgcagatt ctaacaatgc tttaatatta ggaacatcac
     6601 ttcatactgg tattgaaaaa gatattgatg cagcagtaaa agaatattat tttagttatc
     6661 caagtataac agatttacac attaatgaag tgatgaagtt agattattta ataccaaaag
     6721 taaaagaaat tttaccaaaa gggtttaatg aggttcaaat aacaactagt gattttatag
     6781 ggtttataga tttgttagca ccacatgtat acatgactga tacaaactta aatacaaatg
     6841 aaacaaccat atattatgaa gatgaagaaa gagtatttga tatttatgac tttaaatatt
     6901 caaacaacat agatagatat ttagaaagtg agcaacttca tctatataag tatttttatg
     6961 aaaagcaaca tacaaatcat gagataaaag atttgtacta tgtatttgta cctaaaattc
     7021 agattagaca gaagaaaaca gaaaacttac atcaatttag aaaaagatta cttaatgagc
     7081 ttgataaatc tgaaataaaa atagtgaagg ttgagtatga cccaaataaa gttattaatt
     7141 tccttgtaga tataaaaaga tgtcttgaaa acaaggaatt tgaaaaaaat caaaataaat
     7201 tatgtaactg gtgtgactac caagaatact gtgaaaaggg gattgattat atgttattac
     7261 caaatagtga aagaagaagt gtggaaggaa ttaataaaaa aactttatgg atttatggag
     7321 caccatttag tggcaaaacc acattagcaa atgattttga aaagccttta atgcttaata
     7381 cagatggaaa tataaagttt gttgatgctc catacatttc tataaaagat attgtaacag
     7441 ttgaagggag aatgacaaag agacaacttg catgggatgt atttaaagaa gctataagag
     7501 aattagagaa aaaagaaaat gattttgaaa caataattgt tgacttgtta gaagatacat
     7561 acgaagcttg tcgcctttac atgtatgaca agatgggaat aactcatgag tcagatgata
     7621 gttttagagc atgggataag gttagaacag agtttttaag cactataaaa cgacttatga
     7681 accttgacta taaaaacata atattaatat cacatgagga tacatcaaaa gatattacca
     7741 aaaaaggtgg tgacaagata acagcaatca aacctaattt acaagaaaaa gcagcaaata
     7801 aaatagcagg tatggttgat atagttgcaa gagtagtagc tgatggagac gttagaacat
     7861 tatcatttaa gactaatgaa gtgatatttg gagggggtag attaaaagta aaatctaatg
     7921 aaattccact tgattataat gaattaatga aagtatatga tgatattagt atagagaaaa
     7981 aagacgttaa gaagtcaagt aagactaaaa aatctaatgc tgaggaagac gtagaaatga
     8041 ttcctatagt agaagaaaca agtgagaaaa ctgaaaaaga acaaacagat gaagaagtaa
     8101 ttgaacaaga agaagcaatt aaaccagtca agaaaactag aaaaaagaga agttagttta
     8161 agaaagatta ttaattaaaa atttaaaaga aaaggatggt atgtgatatg gattttagta
     8221 aatttgataa agcaatagat gtaaaaggat tgaaagaaga tataaaagaa gcatctgaaa
     8281 atagtggtaa atttaaggat gtaccacatg gcacttatga agtagaaata aataaaatgg
     8341 agttaagcga gtctaaaaaa ggtgacccaa tgtttgtatg ttggtttaaa atactagaag
     8401 gagattataa agattcatta atattcatga atcaagtagt taaacaaggt tttcaaattc
     8461 atatagtaaa tgaattttta agaagtttag aaactgatat tgaagttgaa tttgaatcat
     8521 acagccaata tgcacaactc ataatggata tagctgagga aatagatgga gagcttgaat
     8581 ttgctattga atatggtgag aagaaaggtt ttaatacctt tacaataaaa gacatatttg
     8641 aagtagctta gaaattttat tacagctagg tgtatacatg catctagctg taatattaaa
     8701 aaggtaggta attaatatga gagtaaaaag aaaagatgga acttataaac ccaggtaaaa
     8761 ttgtaagatg tgtttggtgt ggaaaaagat tttacaagtt agataactct aaagtcatat
     8821 attgtagtag aaaatgtgca gcaaaagcga gaggggtgtt ctaaatgatt ttttatgatt
     8881 ttgaagtatt ctcttatgac tggttagttg tatttataga tgttctaaat aaaaaagaag
     8941 aagttattgt aaatgatata gataagttaa attcatttta tatagagcat agagaagata
     9001 tttttattgg ttacaacagt agacattatg accaatatat ttttaaagga ctattatgtg
     9061 gatttaatgc aaaagaaata aatgattata ttattgttaa aggtcaacct ggttggaagt
     9121 tttcaaactt attaagaaac atacaaatta ataactatga tgttatgact agttttcatg
     9181 gattaaaaca attagaaggt tttcaaggtc atagtataaa agaatctaat gtatcattta
     9241 acattgatag accattaact aatgatgaaa tagaagaaac tataaaatac tgtagatatg
     9301 atgttgaaca aactatagat gtttttattg aaagaaaatc agaatttgaa gcacatatgg
     9361 gattaattaa agcttttaaa cttccagtag catatattgg taaaactcaa acacaactaa
     9421 cagcaattat actagaagct acaaagaaag aacatgatga tgaatttgac ttacaaatac
     9481 cagatactct aaaaattgag aaatataagg aagttttaag ctggtataag aacccactaa
     9541 atcatgacta ttctaaaaat ctaaaaatta atatttctaa tgtacctcat gtattcgctt
     9601 ggggtggtgt tcatggagca ataacaaaat actatggtga aggttatttt ttacatgtgg
     9661 atgttaattc cttctatcca agtcttatga ttagatacaa ttaccactca agaaatataa
     9721 aaaatcctca aaagtatgtt gaaatttatg acaaaaactt acagttaaaa aaagaaaaat
     9781 cacctttaag accagcctac aaattggcag ttaataaaac ttatggagga atgaaagata
     9841 aaaacaataa tttatatgac ccaagacagg ctaataatgt atgtgttagt ggacagctgt
     9901 tattacttga tttaatagag aagttagaag ggcattgtaa acttattcag agcaatacag
     9961 atggattaat tgtaaagtta aatacaatag atgattatga gttaatagat gatatatgtt
    10021 atgagtggga acaaaggaca ggcatgggtt taggttttga tgtatacact aaaatatttc
    10081 aaaaggatgt taacaattat ttagttataa cagaagatgg agaagttgaa gcaaaaggat
    10141 tatatatcaa agatttagga aatctagact atgatttacc aattataaat aaagcactta
    10201 aaaattatat ggtaaacaat acacctattg aagaaactat aaataactgt aatgatttaa
    10261 tagagtttca aaagatagta aaaataagta gtaaatattc acatggttta tatagtccaa
    10321 ctatagaggg aaaaacaaaa aaagtattta ttggcggtaa gattttaaat gataaatgtt
    10381 ttagagtatt tgcttcaaaa aataaaaatg atggtggtat ttataaagtc aaaaataaag
    10441 aaaaaaatcc agaaaaattt gctaatacac cagataaatg ttttatagaa aatggaagtg
    10501 ttataggtaa aaaagtacca aggaagctag ataaaatttg gtatatagat ttagcaaatg
    10561 aaagactcaa acagtttgga gtattattaa gtaattgagg tggtgattag tgagttttga
    10621 cctatttaaa ggatatatag ttacaaataa taaaaaggca gctgagaaat ttaaaaatgt
    10681 aaaaaagtta aaaacttatg aacagattaa gaatttacca gagtttgcag gtgttttagc
    10741 agaagaaact gtgttagttg atattgatga ttttgaaagt agtgaaattc tatacaaaat
    10801 agtacaagat ttaaagctaa aatgtagagt ttacaagaca acacgaggta agcattttct
    10861 atttaaaaat actagtttag aaaagaatag aacaaaatgt agactagcta taggtttaaa
    10921 ttcagatata aaattaggat gtaaaaattc ctattcaatt ttgaagttca acaatattga
    10981 aagagaaatt ttatatgata gtaaagaaat acaagagatg ccaatgcatt taactcctat
    11041 aaaaaatgga attgattttt taagtcttgg agaaggtgat ggaagaaatc aagcattata
    11101 taactatatt ttgactctac agagtaatga ttttactgtt gaggaaataa gagaaacaat
    11161 aagagttatt aataaatatg ttttaaaaac tccattacaa gataatgaac ttgaagtaat
    11221 tttgagagac gaatcctttc aaaagaagat attttttaat tcaaaaggtt catttttatt
    11281 tgatgagttt gctaaataca taaaaaataa taatcatgta atcaagataa atgaccagtt
    11341 acatttatat aaggatggta tttatgtaga tggtcaagca agaatagaag cagaaatgat
    11401 taataatata agcaatttaa ataaggctaa gaggagtgaa gtacttagtt atttaaattt
    11461 attaataagt gagaatacaa gtatgtcaga agctaattta atcgctttta agaatggtat
    11521 atataatata gttgatgatt cttttataga attttcacca gaatttatca taacaaataa
    11581 agttaattgg aattacaatc cagtagtata ttcaaagtta gttgataaaa ctatgaataa
    11641 attatcctgt ggggattttg aaattagaat gttacttgaa gaagtagtag ggtactgctt
    11701 ttatagacgt aatgagctta gaaaagcttt tatattaact ggagataaag caaatggtaa
    11761 atctacttat ctggatatga taaagacatt attaggagat gaaaatacat cagcacttga
    11821 tttaaaagag ttgagtgata gatttaaaac tgctgagtta tttggaaaac ttgctaatat
    11881 aggggatgat ataggagatg aatttattgc taatcctgct atatttaaaa agttagtaag
    11941 tggtgatagg gtaaatgttg aaagaaaagg tcagaatcca tttgatttta acaactatag
    12001 taagttttta ttttcagcta ataacatacc tagaataaaa gataagactg gagcagtgct
    12061 tgatagacta attattattc cttttaatgc tagcttctca gtaaatgata aggattttga
    12121 cccttatata aagtacaaat taagagagca agaagcaata gagtatttaa taaacttagg
    12181 gttacaaggt ttgaaaaggg tattacaaaa tagaaaattt acagtcccag ataaggtcaa
    12241 gaaagaaata ctagaatatg aagaagtcaa taatcctatt ttagggtttt ttaaagaagt
    12301 tgataagata gaaaatgaat caacaaaaga aatatatagg aaatatcaag agtattgtat
    12361 attaaatgga ttacagccta tatcaaacat tgagttttca agacaagtag tcaaaaagtt
    12421 tggatatgaa gtaaaagata aaagaataca aggtaagaaa tataaagtat ttactagagc
    12481 agtcccggga tagtcccgga tgtagtccct agatgtaata tagtaatttc aacgttgtcc
    12541 cggatgtccc tagatgtttt tgacctttta tatatttaac taaaaaatca tcaatttttt
    12601 gtgatgattt tgctcccttt atataaagaa tatatatata atagtatggg actgaacaaa
    12661 aattattgtc tggatatatt gaaatagcta ggcttgaagc agtcccggat gtattttaca
    12721 tctgggacta tctgggactg ggtttaatta agaagttata taatgtaaaa atatttaaaa
    12781 aataagatag atttcattaa taacctaata aaataattaa aaagaaaatt ctgttaattt
    12841 gcaaggatgt gaaaatggag tgacagacta tgaaaaatta gaagaattgt taaattcata
    12901 tagtaatttg gatattgaga ttagagaaat agagttaaaa gtaagaggta gtagtttgag
    12961 aggaatagag ttaaatggaa tgcctaaagg aaataatgtt tcctcaccaa tagaaaatga
    13021 gttaatatat gttgaaagat tggagaatga gaaaatatat ctacaaatta aaaaagaatc
    13081 aattaataat atgttaaatt tattagatga ttttgaaaga aatctaatag aactaaggta
    13141 ttttaaaaaa ctacaataca aacaaataag ttatgaatta aatataagcg aagtttatgt
    13201 aggttttaaa agaaaaaaag cattaaataa gatacttcct tttgcaaaaa agtataattt
    13261 aatataaatg ttgtatttat cttgtgttta taattgattt attatagaaa catgttgcat
    13321 caatatattg tgagataaga tataatggta gtatgaaaca agtgtataat atattcccta
    13381 aaaaagacta agttatctcg gcttggtctt ttttaattta aaataaagtg aggtggtgat
    13441 gtggctaaat taactgaaaa acaaaagcgg ttttgtgact attatattga gactggtaat
    13501 gcaacagaag cagcaataag agcaggatat agtgaaaaga cggctaaggt aataggtgct
    13561 gagaacttaa caaaaccata cttaaaaagt tacattgatg aaaaactaaa agaatttgag
    13621 tcaaacagga tagcagacgc aaaagaagtt atggagtatt taactaagat agtaagaaat
    13681 gaagcaaaag aggaagtggt tgtagtttct gaatatggtc cagaaataat aaaaaaagac
    13741 gtaagtataa gagataggaa caaagctgct gaattattag gtaagaggta taggttgttc
    13801 accgataagg tagaagttga aggagctatt cctatagtta taactggaga tgatgaaatt
    13861 gaagattaag aaaataaata tagcaaagct agtaggtaag gggtataaag actattggag
    13921 atttaagggt cgttatagag tctgtaaagg ttcaagagct tctaaaaaat caaagacaac
    13981 agctttatat tatattacta agttaatgca acatccagaa gctaatttat tagttgtgag
    14041 gaaagtgttt ggaacattaa gagatagttg ctataaggaa ctcaaatggg ctatacatca
    14101 gttaggagta gataattatt gggatagtac aactaatcca cttgaaatta catatattcc
    14161 tacaggacaa aagatatact ttagaggatt tgatgaccca cttaaaatta cctctattac
    14221 tgttgatgtt ggagtacttt gctggtgttg gattgaagaa gcttacgaga taacagatga
    14281 aaatagtttt aatatgttag atgaaagtat aagaggtaaa gttccaagtg gattattcaa
    14341 acaaataaca atgaccttta atccttggaa tgagcaccat tggattaaaa aaagattttt
    14401 tgatacaaag gataaggata tactttcaaa gactactaat tatctttgca atgaattttt
    14461 agatgatgca gataagaaag tgtttgaaac tatgaaaaag aacaatccaa ggcgttacaa
    14521 ggtagcaggg cttggtgatt ggggtattgt tgatggatta gtatatgaaa actgggaaga
    14581 gagagtattt aatatagaca ctcttataga gcagaatatc aaatcagctt ttgggatgga
    14641 ctttggttat actaacgacc caacaacttt attttgtggt ttagtgaatg aaccttcaaa
    14701 agagatttat gtatttgatg aaatatataa acatggtatg tctaatgaga gactttatag
    14761 agaagttagt aggaaaggat attctaaaga acatataaca gcagattcag cttctccaaa
    14821 agacattgac catctgagag agttagggtt aagaaatata aaaggttcaa gaaaaggaaa
    14881 agacagtgta aataatggca ttcaatatat acaagattac aagattataa tacatcctaa
    14941 atgtgtaaac tttttaactg aaataagtaa ttatacttgg gataaagata aatttggaaa
    15001 taagattaat aaacctatag atgattttaa ccatttaatg gatgctatga gatatgcact
    15061 ggaagatttc ataaaaggag aggtgtttag ttttgattaa tcttttaaga aagggggtag
    15121 ctaaactgaa taatattcta aataaaccac taaaacaatc agcaaacaat ttaaaatata
    15181 tagagtatga aataaataaa tggaaaacat caaactctag aaaagagcag ttacaaggtg
    15241 ataggtatta taaaggtgag cacgatattt taagacggaa acgtactgtt attggagaag
    15301 gcggaaagtt aacagaagtt gaaaatctac caaataataa aatagtagat aataaatatg
    15361 ctaatttagt agaccaaaag gttaattatt tgctaggtaa accacttacc tttcaaactg
    15421 agaatgaaat atataattct ttattaaagg atatatttaa taagaagttt cttaggacct
    15481 ttaaaaatct ttgtgaagat tctctaaatg gtggtatatc atggttacac ccatactata
    15541 gtgaagatgg taaactaagt ttcaaaaggt ttccgtcata tgaagtatta cctttttgga
    15601 aagattcaga gcatacaata ttagattttg cagttaggct gtatgaagta aaggcatata
    15661 gtggagcaaa tgaaaagata attgagaagg tggaagtata ttcaaaagaa ggtataaaga
    15721 gatatatttt acaaggtatg actctaatag ctgattcaga aagtccatac tcttcttatt
    15781 tagtagttga aactgatgat attcatgagg aacttaattg ggataggata ccactaatac
    15841 catttaagta caataataaa gagataccac ttattaagag agttaagagt ttacaagatg
    15901 gtataaacat aatgttatct gattttgaaa ataacatgca agaagatgct agaaatacaa
    15961 tactggtact tcaaaattat gatggtcaaa atctaggaga gtttagaaaa aatctagcac
    16021 agtatggagc tgttaaagtt cgtactgtag atggggcagt aggagactta aaaacactag
    16081 agataaaagt aaattctgat aactataaat ctatattaga agtatttaaa aaggcattaa
    16141 ttgaaaatgg tagaggatat gatgctaaag atgatagaat gagtggaaat ccaaatcaaa
    16201 tgaatataca aagtatgtat agcgatatag atttagatgc taatggtatg gaaactgaat
    16261 ttcaagcttc ctttgaagaa ctattgtggt ttgtaaatat gcacctttta aatactggtc
    16321 aaggaaatta tgaaaatgaa gaagtagaag taatctttaa tcgagatata ttaataaatg
    16381 aaacagaaag tatttctaac tgtcaaaatt cagttgggtt attatcagat gaaactatta
    16441 tatcccagca tccttggact atagatgtta ggcaggaact agaaaagaag aaaaagcagg
    16501 aagaagctca aagagagtat gatgatttaa ttcctaataa tcaagatggt gttatagatg
    16561 aaacataaag attattggag aaagagattt gaacaattag aagaagctca aaataacaaa
    16621 agtgtaaaat attatcttga attagaaaag caatataaac tagctataaa tagtatagaa
    16681 aaagatatat tagcatggta caacagattt gccaaaaatg aaggaatatc tttattagaa
    16741 gctaagaaac tactaaatac aagagaacta gaagagttta aatggagcgt tgaagaatat
    16801 attaaatatg gtaaagaaaa tgctataaat caaaagtgga tgaaagagtt agaaaatgct
    16861 agtgcaagag ttcatataac aagacttgaa gctttaaagt tacaaataca gcaacaagta
    16921 gaagttttat atggaaatga acttgatggt attgataaac taatgagaga tatttataca
    16981 agtggatact atcatacagc ttttaatgtt caacaaggag taaacgttgg ttggagttta
    17041 atgtctttag atactaataa aataaataaa gttatttcta agccatggac cagtgatggg
    17101 ttaaatttct cagaaagaat ttggggtaaa catagacctg ctttaatcaa tgagttacac
    17161 actaaattaa ctcaatcaat tattagaggt gagaatccta aaaaattagt caatgacttt
    17221 gctaaaagat ttaatgtatc taaatctcaa gctaagaatt taataatgac tgaatcagct
    17281 ttctttgcat cagcttctag gaaagattgt ttcaatgatt tagatgtaga gaaatatgag
    17341 atagttgcca cattagattt aaaaacatca aatatctgta gagaattaga tggtaaagta
    17401 tttgatatga aagattatca agtcggagtt acagctccac cgtttcattg tcgttgtagg
    17461 actacaacag ctccttggtt tgaggatgaa gaaggttaca gagcagcaaa aggaaaagat
    17521 ggaaaaacat attatgtacc atctagtatg aagtataatg agtggtatga gaagtatgtt
    17581 aaaaacaatg atatctcaga aataaaaaat agtgctataa tagatagcat aaaagaagat
    17641 attaaaaatg gtaactataa tttaaatatt catgatggga aacaaggaaa acatttaaaa
    17701 gggcataata attatataga aggaagaagc tatttaacta taacaaaaga agaggctcaa
    17761 gaacttgtaa ataggcatgc tggtaatgga attataaaat ttaatcgaag tggagaatgg
    17821 gataaaaaag aacttataga aattgataag aatatagggg tcaatgttaa taatattaca
    17881 ggtgaaaaaa ctcttacaaa taagtttaag atacattatt ccaaaactgg aacacatata
    17941 gtaccagctt tataaggagg gaaaataaag tgaaattatg ggaatatgta gggaaaaata
    18001 ttcaaataac ttgtgtagat aagcaaataa taagaggaaa gtgtgatggg tatacacaag
    18061 ctttagataa tgaaccagaa atagcaagta tatcaatagc tagggatggt tatggaattg
    18121 aagtttatga aaatgaaata gaatctatcg aaaatataag taaaaaataa ctaagcatgt
    18181 acttaaaaat aagtagatgc ttttattgtg taaaaaatga aaggagatat ttaaaatgga
    18241 ttggttaaaa gaattactag aaggaataaa aatcgaggat aacaaaattg atgtagcttc
    18301 tttacaaaag tctatagaaa agaaaataaa agagactaca gttaccaaag aagaatatac
    18361 aaatcttgaa acacaactta atacagctaa tgaaactatt gaaggtttta agggagttat
    18421 gacaaaacag gaagttgaac aattaaaagc aacttatgaa gctgaaataa aacaaataag
    18481 gagggattct attgatgaaa gattactaac agaaaataga acaagaaata aaaaagcaac
    18541 aaaggcatta cttgaagata ttgaggaaga agattatgaa aagtatgagt ctttaagatt
    18601 agcacagatt aagaaactac aacaaagtga agaaagtaag tttttatttg aagaagttca
    18661 agaaacaaaa gtaaatttta gaggagtaaa tccaggagaa agtaatccta tagcagaaaa
    18721 atcaacagaa gattttactt atgaagattg gtgcaatcaa ttagaaaatt aaagaaaaga
    18781 ggtaaataaa tatggcaaat acaaaatttg acgcaaaatc atttaatgca gaagcatttg
    18841 gaaagtatat ggagtcagct ccaacattaa agagaaatga attattaaaa tcgagagctt
    18901 taaaatcaaa ctcagaaatt agaaacttat ttagtagcca aacaactaca tcatatggaa
    18961 gaatacctat gtatggtaac ttagaagggg atgcacttaa ctatgatggt atgactgata
    19021 taacagcaga tactactgtt acctatgaaa gaggtgttgt agtagttggt agggctaaag
    19081 catggatgga aaaagatttt agccaagata taacagcagg tgtagatttc atggctaatg
    19141 taagaaataa agtagcttca ttttgggagg agattgacca ggatacattg ttatcaatat
    19201 taaaaggtat attctcaatg acaggaactg aaaataagaa gtttgttgat gctcatacat
    19261 cagatataac taaaaatggt gatgggaaag tttctgctga aagccttaat actactatac
    19321 aaaaagcatg tggtgataat aaaaataaat ttagtctagc tataatgcac agtagtgtag
    19381 ccactaattt agaaaattta aatctactaa cttatctaaa atatactgat gagcaaggga
    19441 tgcaaagaga tttaggttta gctacatgga atggtagaac tgtattaatt gatgatagta
    19501 tgccagtaga agatgttgca gaaagtgcaa gtggtgcagg tgatggatat acaaaatata
    19561 ctacttatat attaggtgac ggagcttttg attatgaaga tataggagca aaagttcctt
    19621 atgaaatgga tagagaccct aaaacaaatg gtggtcaaga tactttatat actagacaaa
    19681 gaaaagtatt tgcaccttgt ggtatttcgt atgaaaagaa aaaacaagtt tcattatctc
    19741 caacaaaatc agaacttgaa gatggttcaa actggactct agtaaatgat ggagcaacaa
    19801 ccaaaacaaa atatatagac cataaatcaa ttgcaattgc tcaaataata tcaaagggtt
    19861 aaattttatg gaaaataata taattgatga tatagaaaaa agacttgaaa gctttgaata
    19921 tacattaaaa gatggagata agtggttaat aggttttgtt aaagaaaaga tagagaatat
    19981 aattaaactt gattgtaata taaatgaaat tccaaaagaa ttaaaaaata ttgaaataga
    20041 tatgatagtt ggagagttct tatttactaa gaagaatatg ggtcaattag atatagaaag
    20101 tctcaatttt gaagttgtag aaaagtctat atcagaaggt gatacaaaga tagactttgc
    20161 tataggaagt ggctcacaaa cacctgaaca acgctttgac acttttgtaa actatctttt
    20221 gacttatggt aagaataaga tattaacttt taggtgttta agatggtaaa cagaactaga
    20281 aaagcaatag aaatgttata tagagataaa tgcactatag ttgagtatca gccaattaaa
    20341 gaccctatta caaaacgaac taacaataaa gaagtagtcg tattagaaaa tcaaccttgt
    20401 aagttatcat ataaaaatat tacatcagct acagatggaa aagtagctaa actagagcaa
    20461 actattaaac tctttatatc tccagatata gaaattaaag caggttcaaa acttattata
    20521 aatgataaag agtatgtaag aagtggagaa tctgcgatat atccaaacca tcaagaaata
    20581 atacttgagt tatttaagga taaagcataa tggctagatg aggtagtgtt gattttagag
    20641 agtttaaaag agtttgtaaa aagatggaaa agcttacaaa gattgattta gataagtttt
    20701 gcaaggatgc agcacgagaa ttagcagcac gattacttgg gaaagtaatt agaagaacac
    20761 cagttgatac aggattctta agacaaggat ggaatggagt ggcttatgct aggtcgcttc
    20821 ctgtgtataa acaaggaaat aattatatta tagaagttgt taatccgact gaatatgcaa
    20881 gttatgtaaa tttcgggcat agaactaaag atggtaaggg atgggttaaa ggacaacatt
    20941 tcttaacaat ttcagagatg gaactacaaa gccaagttga taagattata gagaaaaagt
    21001 tattaatatt acttaaagga gtatttgatg cttaataata taattgatgg aatatctatt
    21061 aaattagata aaacatttgg agagagttat acaatttata gtgaagatgt ggagcaaggt
    21121 ataaatgaac cttgtttttt tattgttcct ttaaatccaa gcaaagtatc atatccaagt
    21181 ggcaggacat taaaaaagaa ctcttttgat gtacattatt ttcccaaaag taatgataaa
    21241 tcatttgaaa taaatgaggt agctgagatg ctactggagg aattagagta tatagaaatt
    21301 gatggagatt tagtcagagg tacaaatatg aactttgaaa ttatagataa tgttcttcat
    21361 ttcttcgttg attataacta ttttactata aaaaataatg ataccaataa gatggataca
    21421 gtagagttat tcggtggttt gaagagaggt gataattttg agtaaagcat taagcaaaga
    21481 agataactac aagtttacta aggagcagat agttaactct aagaagtatg taaataaaaa
    21541 agatttatta aatgcaattt taaaagaaaa tgagttatat tccttctcag aggtagagga
    21601 tagaataaat aaatttatga aaggagtgag ttagatggct ttaggtggag gaacattcgt
    21661 aacgcaaaat aaaatacttc ctggtgcata tataaatttt atctcagcta agagggcaac
    21721 cagttcatta tcagatagag gtattgttgc aatgccttta gagttagatt ggggcataga
    21781 tgaagaagta ttccaagtaa ccagtgatga ttttgagaag tattcagtga agtattttgg
    21841 atatgattat actcatgaga agctgaaagg tttgagagat ttattcaaaa atataaggtt
    21901 gggatatttt tataaattaa ataaaggcgt taaagccagt tgtactatag ccacagcaaa
    21961 atatagtggt atcagaggaa atgacttaaa agtaacagtt acaacaaata tagatgataa
    22021 tgctaagttt gatgttgtaa cacttttaga taataagaag gtagatactc aaatagcaaa
    22081 ggttattaca gacttacaag acaatgacta tatcacttgg aagaaggatg caacactaga
    22141 agcaagcgca ggacttgtat ttactggtgg aactaatggc gaagctgtga caggagcaga
    22201 gtaccaagct ttcttggata aaatagaaag ctatagcttt aatgctttag gatgtttggc
    22261 tacaacaaca gaaattaaaa gtttatttgt agaatttaca aggagaatga gagataaggt
    22321 aggagctaag tttcaaacag tactatataa gaaaagtgat gcagattatg aaggtgtagt
    22381 gtctatagaa aataagatta aagataaaga cttagttgaa tctagcttaa tttactgggc
    22441 tactggagca atagcaggat gtgatataaa taaatctaac actaacaagc gatatgatgg
    22501 tgagtttgat gttgatgtta attatacaca aatacaactt gaagaagctt taaaaactgg
    22561 taaatttata tttcataaag ttggtgatga agttcatgtg ttagaggaca taaatacttt
    22621 tgtatcattt acagatgata aaaatgacga tttttcaagt aaccaaagtg ttagagtact
    22681 tgaccaaatt gccaatgata ttgcaacttt atttaatgaa aagtacttag gtaaagttcc
    22741 aaatgataag gcaggaagaa taagtttctg gaatgatgtt gttaaacacc ataaagaatt
    22801 agaaaacatt agagcaatag aagattttaa aactgatgat gttagtgtag agcttggaaa
    22861 tgataagaaa actgtcatag tatctgatgc tgttaaggta ataaatgcta tgagtaagct
    22921 ttatatgact gtttcagtta gttaagagag gagtgtgata atatgtttca gcaaataaaa
    22981 gcaagagata caataagtgc atctaaggca gaatgctatg ttactatcga gggtaaaaga
    23041 tataacttta tgcaagctat taacttagaa gctaagatgg aaaaaaataa aagtgaagtt
    23101 cctattttag gaagcactac aaaaggtaac aaaagtacgg gaagtaagta tacaggaagt
    23161 gcaacatttc actacaatac ttctatattt agagagcttc tttataagta taaagagact
    23221 ggtgaagata tttattttga tatacaggta acaaatgaag acccaacgag ttcagctgga
    23281 cgtcaaacta taatacttaa ggattgcaac atggatagtg gaattatagt taaatttgat
    23341 gcagatggtg agtatcttga tgaagatatg gactttacct gtgaagattg ggaattggtt
    23401 gaaaagttca atataataaa cggaatggag taaaatacac atttataaat tatatatgtg
    23461 tattttttat atgaaaaatt aaaataaaag gagattagaa taatatgagt aatttaagtg
    23521 cttttttaag tcaaaatgca ataaaggttg ataatgtaaa atatatagtg agtaacagat
    23581 ttttagataa agaagggaaa ccagttgaat gggaattaag agttttatca tctgaagaag
    23641 acgaagcact aagaagaaaa tgtactaaaa gagtaaaagt gattggtaac aatggtaagc
    23701 atactggaca atatacaagt gaaattgact acaatagtta tgtagctgaa ttatgtgtag
    23761 catctacagt atttccagat ttaaaggatg ccgaactcca aaatagttat ggagtaatgg
    23821 gagaagctca gttattaaag acaatgctta cagcaggtga gtatgtcaat tatacagtaa
    23881 aagtgaatga agtcaatgga tttgatacat cttttgagga taaagtagaa gaagcaaaaa
    23941 actaatcaga ggtggcgatt ttgatgctag catcactcat tattgtattc aaaaattaaa
    24001 gtggaagcca agtgaatata tgggattaga gattaatgag agagcattag cagccgcctc
    24061 aatacttata aagatagagg atgaagagga agcaatgaaa gaagctgaaa gagagaaaaa
    24121 gagaggtaga agaagataat ctctgatgta aaaattttac taatatagta taatatattt
    24181 aaaaattgta ctgggggatt aaataaatgg gattgtttag taaaaagaat aagaaacctt
    24241 gttgtatttg cggaagtgaa aaagggctta tgccatctat agaaggtgaa aatttttgta
    24301 cagcttgtaa ttgcaagtac atagatttct ctgaaaatat tttaaaggta actagtatta
    24361 tgaaaatgat ggcaaactta gaaggaatga aaaagtttat agaagttgaa aagaaaaatt
    24421 taaaattgtt agagaagttc accgagacaa aaagtataaa ttcttcaatt tcttttgatg
    24481 aagagcaaaa tcttttaaaa atatcatata aaaatagaaa tcaaatatta gtagagaaaa
    24541 taataaaatt tgatgatata ttagaatttg aacttttgga agatggagaa actatagtta
    24601 aaggtggttt aggtcgtgct ataactggag gagtcttatt cggaggtaca ggggctgtag
    24661 ttggaggaat tacaggcaaa aaaacaagta gaaaagttgt agaaatactt aaaataaaaa
    24721 taactgtaaa ggacataaat aatccaattg aatatataaa tttaattaat agtaaattaa
    24781 aaacaaactc ttctatttat cagaaagctc tttctgatgc tcaagaaata ttatcaatac
    24841 tatcaataat tacaaaatat aatgtaatgg aagataaaaa agaagagtct atgtgtaact
    24901 ctacagctga tgaaatatta aaatataaga atttattaga tatggaagct attacacaag
    24961 aagaatttga tgctaagaaa aaggaattat tgaatttata ataataaaag cacttactta
    25021 aaaacaggta agtgctttta ttattatcaa aaagaaagga gagtgagaaa atggcaacta
    25081 tacaaacttc aatccgaatt ttcgatggaa tgacacctgc atttcgacat atgacaactg
    25141 ctatgaatat agtattaagt tcctttgaac aattacaaag aacatctagt aatgcagtaa
    25201 atgctaatag cataataaga gctagagaag aattggcaag agcagaagct ggatttgata
    25261 gattagaacg acaaataaga gaagctgatg ggcaacagcg aagacttaat gaggatataa
    25321 ataagggtgc aagttctaca gatagattag ttggaagtgc aaagaagcta gcagcaactt
    25381 acttaggtat aagaacgtta ggaggtttag gaaatttaag cgaccagatg acaagtacta
    25441 acgcgagact tagtatgata aatgatggtc aacaatcaga tagtgggctt agtaagatga
    25501 tatttcaaag tgctgagagg tctcgtgcat cttacttaga cacagcgcaa atagtctcaa
    25561 ggataggcat gaacgcagga agtgcattta gcaacactag agaaatagta agctttgcag
    25621 agcaattaaa caaaaaattt gtaatcgcag gagctagtac tcaagaaatg agttcagcat
    25681 tattgcagct aacgcaagga ttaggttctg gagtactgag aggagaagaa ctgaatgctg
    25741 tatttgagtc agcacctaac attatccaat ctatcgcgga ttatttggac gtggatatag
    25801 gaaaaattcg aggtatggca agtgaaggaa tgttgacagc agatattgta aaaaattcct
    25861 tacttgctgc atcagctgaa acaaataagc aatttgaaca gatgccttat acacttggtc
    25921 aaattttcac tagtgtaaaa aataatgcag tcatggtatt tggagctata cagaagaaaa
    25981 ttgaggacac agtttcaagc cggggatttc gaactttcat aactgatgtt aaagactcat
    26041 tatatgtact tggagcagtt gggtttaatg tatttagtgg atttattaat ttactgagta
    26101 gtccagcttt tcagaatttt tttaatgtga tgattgttgg aactagttta gttgtacaag
    26161 ggctaggttg gataataaca caagcactta gtgttgctaa tatattttcg cgaaattgga
    26221 gtataatttc acctataatt ttaggtgtgg tttcagctat tagtacctat ataatagctc
    26281 ttggtattat gcaagcagtt actatagctg gaactgtagc aaattggatg tcatggacat
    26341 ctgaagttat tagacaaaga ggaattttag gaacagctag agctttagca ataggcaaag
    26401 ctgaacaatg gggatttaat gctgctatat atgcatgtcc tattttttgg atagttttag
    26461 gtataattgc agtaatatct gctgtattta tattagtagc tgtgtataat cattttgcag
    26521 gaacaaatat aagtgtaata agccttgctg ttggagcttg gtattggtta tgtgcagttg
    26581 tttataacat aattgcaggg atagtaaatg cgataaatat ttgcgtagtt gaaatagtaa
    26641 atatctttag aactggctta tatgcagtgc aatgtttctt tattgatatg gctaacgcag
    26701 gactcaaggc aggtgttaat ttagacaaag cttttgataa atttgctacc aatctagcaa
    26761 atggtattat aaaggctgtt aatatagctg taaaaggatt gaattggtta gtgcaacaga
    26821 ttaataaaat tccaggactt gatttgccac aaatgaaaga atttcaaaaa gtaaatactg
    26881 taattggtac aaaaacaaca tttaaaccca ttcaaaaacc gcctgagcct aaagcatgga
    26941 aaacagaatt agtagagtac aaaaatttaa agtcagagtt tataaaagga tatgatgtag
    27001 gacatcaatt acaaaacaaa ctgaaagaca cttttgacat tagcaaaata gcagaagatg
    27061 caaagaaaaa gcttggactt gacgaccttt gggacaaaaa atatggtctt ggcgattcaa
    27121 taggttctgc aggtcttaac tctccactca gcgacgcagc aaaaggagca aaggatacag
    27181 caggaaatac agcaaaaatg gctaagacaa tggataaaag tcaagaagac ttaaaatact
    27241 taagagatat agcagaacag gaaacaataa acagattcac aggagtaaac attaaaattg
    27301 acatgaacaa cacaaataac ataagtaaag atactgactt agatggaata gttaatgtac
    27361 taactgaaaa attaaacgat gcaatggttg tatcagctga gggaatagtt taggaggtgt
    27421 agtcgtggct tatgattttt atttagatgg agtacaatta ccaatcacac ctggcaaatt
    27481 agaagtaaag ataacaaaca aaaataagac agttgattta ataaatgttg gagaagtaaa
    27541 catattaaag actcctggtt tatcagagtt ttcatttgaa gcagagttta cacacaataa
    27601 actgccgttt tatcgtggtc aatttaaaga tgtacaattc tttttaagta aactagaact
    27661 attaaaaaca gattgtaagc cattccaatt tattgtatct agacaacttg ggaataaagt
    27721 actatttaat acaaatagaa aagtatcact tgaggaatat aacatagtag aagatgcaga
    27781 aaatggttca gattttaagg tagcaataaa gttaaaacaa tatagagatt actcaactaa
    27841 aaagttagta cttgcaacac ctgaaaagac aaattatggt aggactcccc ctccagtcat
    27901 gaaaccaaaa gaatttagac cagattcatc caataagcca aatgctaaga catatacagt
    27961 caaggcagga gattctcttt gggcaatttg tcaaaagcaa ttaggaaatg gttcactata
    28021 taagaaagta tatgaactaa ataaatcaat gatggataaa gctaacaagg gtaaaaaagt
    28081 acctaaatat accatctata aagggcaggt gttaaaactt ggctgatgat ttagttcttg
    28141 caaatgatag agatataagg ttagttatcg cacactggga agatttctat gagccagttg
    28201 tccttgataa cataacatgg gaaatagaaa gaagaggttc accttctaag ttagagttta
    28261 caatagtcat ggatgatata ctacaattct gtgaaggtaa ctctgtaagg ctgtattata
    28321 aaggaatagg tatattttat ggatatatat ttcagaagaa aagagataaa gaaaatcaca
    28381 ttaaaattgt tgcttacgac cagttaagat attttaaaaa taaagatact tacgtatata
    28441 gtaataaaac tgcaagtgaa cttgtaaaaa tgttagctaa agattttaat ttaaaataca
    28501 atgtcataga agacactaag tataaaatat ctagagttga agaaaataaa acactctttg
    28561 atatgatttt aactgcactt gatgatactc taagagagaa aaaggaaatg tatgtactct
    28621 atgatgattt tggaagatta acattaaaga atgttgcttc tatgaaactt gatactgtta
    28681 tgaataatga tgtaatagaa gattttgact ataattcatc aatagatagt gatacttata
    28741 caaaaatcaa acttgtaaga gataatgagg aaacaagcaa aagggatgtg tatattgctc
    28801 aagattcagc tcacatgaga agttggggaa tacttcaaat gtttgagaca gtagacaaaa
    28861 atatgagtga agcagagata aaacaaaaat gtgatatact tctaaaacta tataataaga
    28921 aaactaagtc attaagttta aaaaatgtac ttggtgatat tagagtgaga gcaggttgtt
    28981 tagtacctgt ttttttagat ttaggagata ttgaattgca aaattatatg ttagttgaga
    29041 aagtaaaaca tacatttgaa aataattcac atttcatgga tttgaccttg gttgatggag
    29101 acgaatttgc ttcttattct tcatcttcat atagtagtgg aaatacaaat aataaagatg
    29161 aaaagaaaaa tggtcctgca caaagtacta caagtaaaga agacaatgat atgataaata
    29221 aattaaataa agtatttaaa aataagttat caaatacagg aagtatattt gttaaatatt
    29281 ctaatgctta caaagtcaat gcagctttaa tggctgctat ttctatacat gaaactggta
    29341 atggaagttc ttcactttgt aaaaataaaa ataatttctt tggaatgaaa ggaatgtctt
    29401 ttggctctgt agatgaagga ataaaaagag gtataagtaa tttatc
//
LOCUS       DAEMHZ010000041        28723 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000041 DAEMHZ010000000
VERSION     DAEMHZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.041"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..791
                     /locus_tag="KPK72_001683"
     CDS             246..791
                     /locus_tag="KPK72_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG2488910.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            823..1959
                     /locus_tag="KPK72_001684"
     CDS             823..1959
                     /locus_tag="KPK72_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG2488911.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            1963..3354
                     /locus_tag="KPK72_001685"
     CDS             1963..3354
                     /locus_tag="KPK72_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG2488912.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            3430..4305
                     /gene="dpsA"
                     /locus_tag="KPK72_001686"
     CDS             3430..4305
                     /gene="dpsA"
                     /locus_tag="KPK72_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG2488913.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            4308..4898
                     /locus_tag="KPK72_001687"
     CDS             4308..4898
                     /locus_tag="KPK72_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG2488914.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(5105..5305)
                     /locus_tag="KPK72_001688"
     CDS             complement(5105..5305)
                     /locus_tag="KPK72_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488915.1"
                     /translation="MKKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            5809..8463
                     /gene="adhE"
                     /locus_tag="KPK72_001689"
                     /gene_synonym="adhC"
     CDS             5809..8463
                     /gene="adhE"
                     /locus_tag="KPK72_001689"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2488916.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     KNVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            8740..10958
                     /locus_tag="KPK72_001690"
                     /pseudo
     CDS             8740..10958
                     /locus_tag="KPK72_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            11212..11874
                     /locus_tag="KPK72_001691"
     CDS             11212..11874
                     /locus_tag="KPK72_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488917.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            12245..13654
                     /locus_tag="KPK72_001692"
     CDS             12245..13654
                     /locus_tag="KPK72_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG2488918.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            13769..14044
                     /locus_tag="KPK72_001693"
     CDS             13769..14044
                     /locus_tag="KPK72_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG2488919.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            14674..14985
                     /locus_tag="KPK72_001694"
     CDS             14674..14985
                     /locus_tag="KPK72_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488920.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            14987..16912
                     /locus_tag="KPK72_001695"
     CDS             14987..16912
                     /locus_tag="KPK72_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG2488921.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            16915..17406
                     /locus_tag="KPK72_001696"
     CDS             16915..17406
                     /locus_tag="KPK72_001696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG2488922.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            17458..18021
                     /locus_tag="KPK72_001697"
     CDS             17458..18021
                     /locus_tag="KPK72_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG2488923.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            18036..19013
                     /locus_tag="KPK72_001698"
     CDS             18036..19013
                     /locus_tag="KPK72_001698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG2488924.1"
                     /translation="MAEEKTYPCAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            19000..19323
                     /locus_tag="KPK72_001699"
     CDS             19000..19323
                     /locus_tag="KPK72_001699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG2488925.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            19440..21218
                     /locus_tag="KPK72_001700"
     CDS             19440..21218
                     /locus_tag="KPK72_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG2488926.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            21215..22588
                     /locus_tag="KPK72_001701"
     CDS             21215..22588
                     /locus_tag="KPK72_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG2488927.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            22594..23262
                     /locus_tag="KPK72_001702"
     CDS             22594..23262
                     /locus_tag="KPK72_001702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG2488928.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            23539..24588
                     /locus_tag="KPK72_001703"
     CDS             23539..24588
                     /locus_tag="KPK72_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2488929.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            24684..25673
                     /locus_tag="KPK72_001704"
     CDS             24684..25673
                     /locus_tag="KPK72_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2488930.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            25678..27396
                     /locus_tag="KPK72_001705"
     CDS             25678..27396
                     /locus_tag="KPK72_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2488931.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSIISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVSIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(27478..28632)
                     /locus_tag="KPK72_001706"
     CDS             complement(27478..28632)
                     /locus_tag="KPK72_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2488932.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
BASE COUNT        11027 a         3389 c         5080 g         9227 t
ORIGIN      
        1 atatatcata ttataattta caagtatagt ctaaatagag ctgtttttaa gcaaagtttt
       61 ataaatgact attaaaatat aaagtaacag tataaagttt acttataaat taaatgtaaa
      121 cctaaatact tataatagtt gacaaaaaaa cgtctttatt ttatactaaa acaaagcttt
      181 aatagaagct ttgtatatgt acatataaac taaatgaaat aagacatatg aagggaatta
      241 taaaaatgaa aaatactaaa gtaaatattc aagatttaac taaaatgtca atatgtgtag
      301 cattattgtg tatatcatct tacatatata taccattgcc atttactcca gcaggagtaa
      361 cagcacaaac aataatgata aatttaatag ctttgatttt aacaccaaga caagcattta
      421 gtacagttgg tgtatatata atgataggtt taattgggat tccagtattt ggtggaggta
      481 catctggtat tggaaagtta ttaagcccaa caggtggttt ttattttggg tttttgtttg
      541 cagcactaat tattagttta ttaaaaggac gaaataatga tattaagaga tatatactaa
      601 taacaatttt tgttggaatg cctattatat attttatggg aacagtattt atgtgctatt
      661 ttaatcaaat gacagtagaa gcagcacttt ttgcagcagt agtaccattt ttaattggag
      721 atacaataaa gtcggttatt gcatcttttt taggcactaa actgaacaat gtactcagaa
      781 gaaatctatg ataaatctat aatataagga agggtgtaat gtatggagga agtttttata
      841 ttaggaggat tgagaagtca tataggtcta aaaaatggaa tttttcaatt tgttcaacct
      901 gaattactag gagcaagtgt tttaaaagat ttaattaaaa agtatgaaat tgataaaatt
      961 gatgagatta tatgtggaaa tgcagttggg acaggaggga atatagctag attaatgact
     1021 ttaacagcag gagtatcaaa tgaagtacct gcttttactg tggatatgca gtgtgcttct
     1081 gctatgatga gtatagacat agctttttca aaggttaaat ctggtcagtg tgatttaatt
     1141 attgcaggtg gttttgaaag tagttctcta caaccaatgc gcacatatca taagaatgat
     1201 aagcgatata atacaaataa tccaaattat acagttgctc aattttctcc tgatgataat
     1261 agccaaaata gtatgctcga aggtgcggaa agagtagcag agttatatca aattgagaaa
     1321 gcagacttgg atttttgggt aaaggaaagt cataaaagag caaaagaagc aagagaggaa
     1381 aaaatacttg aagatattat atctcctata aataatagta cctatgatga aggtatacgt
     1441 gataagatga gtcaaaggtt attagatagg atgccatcaa ttttaggaaa agaaactatt
     1501 acaaatgcag caaattcatg tcttattaat gatggagctt catttattat aatatgttct
     1561 aagaagtatt tagaacatgt aaaaaagaag cctaaagcaa aaattattaa tacatgcacg
     1621 atagggacag atgcaacctt aagtccaaca tcagcgatac aagcaatgga taaactttta
     1681 gagatagaaa gccttaatta tttagatgta tctgctgttg aatttaatga agcatttgca
     1741 gttatagatg tgttgtttca aagaaagtat ccagagttaa tagatagata taatatattt
     1801 ggtggagctt tagcgtatgg acatccatat ggtgcttcag gtgcaattat tgcattacat
     1861 cttttaaaag cattagaaaa gactaagggt agatatggaa tttgctctat agctgcagct
     1921 ggtggtctag gttcagcttt actgcttgaa agggtgtaga ttatgaacta ttatgaactt
     1981 ttaagaataa aaaaagagag gcataaaaat aaaaattttt taattataga tggagaaaaa
     2041 tatacttatg aaaatatatt gagttatagt gaggaactag gaaaacaaat ttctttaggt
     2101 gaaaatatcc caattttaat atattctaaa aatataatgt ttcagttgat tagttttttt
     2161 gctattaatt attcaaaaaa tattcctatt atttgtcatt ataatctttc taaaaaagta
     2221 ttagatgata ttttattaaa aaataatatt tcaatcatta tatctgatga atctatggac
     2281 gttattgatt tatatgatga taacaataat gaagtagcaa aaaaagttaa tttttctatt
     2341 tttaatccta aatttaatat atatatgtat caatataaga agcttataaa ttatttagat
     2401 aaaagtattt gtatgggagc tttatcttca ggttctacaa gtgttccaaa ggtattatat
     2461 agaacatatg agagttgggc tggatttttt cctatacaaa atggtatttt taatatctct
     2521 ggggaatcaa tcttgtttgt taatggaaca cttagtttta cagggaatct aaattctata
     2581 atgtcagtat tatatgaagg aggtagtatt gtaataagtt caggtcttaa ttgtaaatca
     2641 tggataagga caataaaaca atataatgta actaacatat accttatacc tacaaaactt
     2701 caacttttgg ttaaacactt aaaagaacca gtactcaaag ttgtaagtat ttttacaggg
     2761 tctcaattat tatttgaaga tactgcaaga aatctaaaaa aatatatgcc aaagtctgaa
     2821 attattttat actatggggc aagtgaatta aattatatta catatttatg ctatgatgaa
     2881 ctaattgaaa aacctctaag tgtaggcaga ccatttccag gaatagatat atatattaga
     2941 gaaggaaaaa tttttgtgaa tacagaatat gcagtatata atgcaaccaa accttattct
     3001 gtcaatgata ttggatatta tgataatgat ggatacttaa tatttgaagg aagaagtgat
     3061 gacattataa atattggagg atttaaagtt agttctacta agattgaaaa tgaagttaaa
     3121 aaaattcctc aaattgaaga tgcaattgta ttgccgtact cagacactat aagaggaaat
     3181 caaattgtat tatttgtaat aactatagac aaaattacga agaaagattt gttgattaaa
     3241 atgaaagata atctaatgaa aaatgaaatc ccaaagaaaa ttatcttttt aaattcattt
     3301 ccatatacct cttctgaaaa gatagataga ttagcattat taaaaatgtt ataagtataa
     3361 tatttagtaa ttttagtgaa ccaatgtatt ggtaaaaaca tattgtatat agtaaaggag
     3421 gcttacaata tgtcaaatca atacgatata actgttattg ggggagacct gcgacaagtg
     3481 tacatggtaa aaaaactaat taataaaggg tattctgtag tcacttatgg gatatcaaat
     3541 gaaattataa atggaatcgt tggaaaatca acttcactag ctgaagcact atccaaaagt
     3601 tccattattt tatgtccaat tccatttaca aagaatcaag ttaatattga aaatacggat
     3661 gattgtcttg atgctactat tgataacttg ctaattaatt tatctcctaa tcaaatatta
     3721 tttggaggta acataccaac taaagtttgt aaattttgcg agcaacactc tattcagatt
     3781 tatgatttta tgaaaatgaa tgatgttgct attttaaatg cagttgctac agctgaaggg
     3841 gctatagcag aagctattaa aagaagtata atcaatatac atcaaagtaa ctgtttagta
     3901 ttaggttatg gaagatgtgc acaaatactt gcaaaaaaat tatacgcttt ggatgcaaat
     3961 gtatgtgttg gcgcaagaaa taatgatgca agaagtacgg ctaatgcatt tggatattcc
     4021 agtatttctt taagtcaact tgatgaaaaa cttttacaat ttgattatat ttttaacacc
     4081 ataccagagt gtattttaac gaaagaaagg ttagaaaaag tatcacaaga agtaactatt
     4141 atagatatag cttcttctcc tggaggagta gattattctg ttgttaaaga agcagggatt
     4201 aatgcgactc tttgccttgg gcttcctggt aaatattcac caaagacatc tggtgaaatt
     4261 cttgttaacg ctatagaaaa tattataaat gaaagaaggg attagaaatg agacttgaag
     4321 gacttacaat tggagtagga tttactggtt cgttttgtac ttatgataaa atatttatag
     4381 agttagagaa tttggttaaa gaaggagcta atgtacacac tatattttca gatgtatcac
     4441 aaaatatcga ttgtagattt ggaaattctg aagagtttat gaaaaaagca tatgaactta
     4501 caggaaataa acctatagtt acgattgaag aggcagagcc atttggacct aaaggaattg
     4561 ctgatattat aataatagct ccttgtactg gaaatactgc tgcaaaatta gcaaatggta
     4621 ttacagattc accagtatta atggcagcaa aaggtcatct tcgtaatgat aaaccactag
     4681 taatatcaat atcaacgaat gatgctctaa gctttaattt caaaaatatt ggcattctcc
     4741 ttaattctaa aaatatatat tttgtacctt ttggtcaaga taattgcaaa gcaaagccta
     4801 attctatgat tgcacatact gagctaataa ttccaactat agaacttgct ctagagaaca
     4861 aacagctaca gcctgttata aaaagtccac atcaatagaa ttggttctta taatattatg
     4921 tattagataa aatactagca aagaggctgt ctcaaaatag agagaagcct ctttttcttg
     4981 ttgaaatgat gaaaatatat ttaatttcat acaaaaaaat gcctcatgga ctaaaaagtc
     5041 tattttgagc taatttccgt aatttttggt atattaaaat tatttactta atccaatttt
     5101 taaattagaa tttatatcgt tttgactctt caaatatcaa tctaactgtt gttgatacaa
     5161 aagaagagga cataattaaa acaaataccg atagtcctct aataaacaaa ttatctacat
     5221 aataagttac tattccaata cctccaatcg ttaaaattac acttataatc aaagaaaaaa
     5281 cgctaattgt ttttaatttt ttcatgctta tcctccttct tataaacttc aaatttatct
     5341 atataatatt aaatatattt tatttcatag tatataaaaa tcatgaatat atattttttt
     5401 aattatacta tatgttgtaa atgtttgtac atttaactat atttacttta acttgttaat
     5461 taagttttaa ttttcatttt caaggatttt tatttttaaa cttgatagag tatcatatta
     5521 aaaattcttt ttataaagtt ttatttgcaa aaaatacaaa aatataaacg ttaaaaaaat
     5581 aacgttatcc ttaagctttt agatgatttc attagtgatt tgcgtaaaaa ataaagtaat
     5641 atctagttaa attaagtatt tgtaaagcta gatattatta gtagaaatga tgttatatta
     5701 gataaaaaat aaaatgttaa aaaaataact tgaaaataga aaaagtaaat gctataatgt
     5761 taataaatta acataataaa taaaaaacta tctcaggagg ttgttaaaat ggctcataaa
     5821 gaacaagtaa taatagaaaa taaaaaagaa aatgaagtta tgtcagttgg tttagatact
     5881 gtaaataacg ttgaaagcct agttaaaaaa ctagcaaaaa tcagagaagc tcaaaaaata
     5941 tttgctacat atagtcaaga gcaggtagat aaaatttttt tagcagcatc tttggcagct
     6001 aataagcaaa gagttcctct agctataatg gcacaaaaag aaactggtat gggaattgct
     6061 gaagataagg ttataaagaa tcattatgcc tcagagtata tatataatgc atataaagaa
     6121 actaaaactt gtggtgttat agagaaagac gaagcatttg gtatgactaa aattgctgaa
     6181 ccaataggtg ttattgcagc agttgtacct acaactaatc caacttctac tgctatcttt
     6241 aaagcacttt tagctttaaa aactagaaat ggaataattt tctctccaca tccaagagca
     6301 aaaaattcta ctatagaggc tgcaaaagtt gttcttgagg ctgctgtttt agctggagct
     6361 cctgaaggta taataggatg gatagatgag ccatctttag aattaactac aactgttatg
     6421 aaagaagtgg atttgaccct tgctacagga ggtcctggaa tggttaaatc agcgtattca
     6481 tctggaaaac cagctattgg agttggtgca ggtaatactc cagcaataat tgatgatagt
     6541 gcagatataa aaactgctgt aaactcaatt cttgtatcta agacttttga caatggtatg
     6601 atatgtgctt ctgaacaatc ggttatagta ttagaaaata tatacaatga agtcaaaaaa
     6661 gaatttaaag agcgtggtgc ttatttatta gataaagatg aaacagaaaa aataagaaat
     6721 ataattttag taaatggagg attaaattct aaaatagttg ggcaaacagc ttgtactata
     6781 gcaaaacttg caggctttga agtcccagta gatactaagg ttttaatagg tgaagtagaa
     6841 tcagttgaaa tagaagaagc ttttgctcat gagaaacttt caccagtact tgcaatgtac
     6901 aaggcaagtg attttgatga tgcagttaga aaagcagaaa aattagtaga agatggtgga
     6961 tttgggcata catcttcatt atatattgat gatgtaaatc aaagagaaaa acttgataag
     7021 tttacttcag ccatgaaaac ttgtagaatt ttaattaata ctccttcttc tcaaggtggt
     7081 ataggggact tatataattt taaactagct ccttctctta ctttaggatg tgggtcttgg
     7141 ggtggaaatt cagtatctaa aaatgtagga gttaagcatt tacttaatat taaaacagta
     7201 gctgagagga gagaaaatat gctttggttt agagcaccag aaaaagttta tttcaaaaaa
     7261 ggttgtttag gagtagcatt aaaagaactt aaagatgtaa tgaataagaa gagagctttt
     7321 attgtaacag atacattcct ttacaataat ggatatacta aagcagttac tgatttatta
     7381 gatgaaatga atattaagca cactacattc tttgaagtag aaccagaccc aactttggaa
     7441 tgtgctaaga taggcgctaa agctatgaga gaatttaatc cagatgtaat aatatctata
     7501 ggtggaggaa gtgcaatgga tgcaggaaag ataatgtgga cattgtatga gcatccagat
     7561 gtagatttcc aagacctagc tatgagattt atggatataa gaaaaagggt atatacattc
     7621 cctaaaatgg gtgaaaaagc taattttgta gctataccaa catcagcagg tactggttca
     7681 gaagtaacac catttgctgt tataactgac caagatacag gagtaaaata tccattagca
     7741 gattatgaac tgatgccgaa tatggctata gttgattctg atatgatgat gaatatgcca
     7801 aaaagcttaa catctgcttc tggtatagat gctttaactc atgcattaga agcatatgta
     7861 tctatgcttg caaccgatta tacaaatgga ttagctttac aagctattaa gagtatattt
     7921 gaatatcttc cacgtgcata tgataatggt gcaaaagacc cagaagctag agaaaaaatg
     7981 gcaaatgctt ctacaatggc aggtatggct tttgcaaatg catttttggg tgtttgtcac
     8041 tctatggctc ataaattagg tgctttccac catgtaccac atggtgttgc aaatgctctt
     8101 ttgataacag aagttatgaa atttaattct tcagatgcac caaagaaaat gggagcattc
     8161 tctcaatata aatatccaga agcattaaag agatatgctg aaatagcttc atttttagga
     8221 ttaaaaggaa attctgatga agagaaattc caaagtttat tggtagctat tgaagattta
     8281 aaacttaaag ttggtattcc taaatctata aaagagtttg gagttgaaga atctaagttt
     8341 atggattcaa tagatgaaat ggtaattcaa gcatttgatg accaatgtac aggagcaaat
     8401 ccaagatatc cacttatgaa tgaaataaaa gatatgtatt taaattcata ttatggaaga
     8461 taagtttgct ataaaataga taaaataaaa actcttatac ataaaacaaa gttttattgt
     8521 ataagagttt tttttgtttg agaaaatgga tagtttttat tctttaattt aaaattcaat
     8581 taaatagtgt tattataata ataaaagaat gtttttatgg ttattatgtt gattttatgt
     8641 gtaacattta agaatagtta tatacaatat aaatgatgaa ttggtataaa aaggatatat
     8701 tatctatatg aagtattgac taattagggg aggagtcgaa tgacagggag aaataaaagt
     8761 tttacattaa ttacaattct ttttttaaca tcattatttt tgacaatggg gtatatctat
     8821 atagaagata caaaacatct tcttatgtca gaggcaaaaa tacatataaa agaagttgca
     8881 gtacaaggct ctcaactagc acaaagacaa attgaaaaag atttagatgt tttaaatatt
     8941 ttttctaact attatgcttc aaatccagat atacctaatg aggaaaaaat gaaaaatctt
     9001 ttagatgaaa tggaaaatca aaaattttac actatggcaa tagttgatat aaatggtaat
     9061 gctgaaaata ccaaaggtga taagttttca gtgaaagata gagaattttt caaaaactca
     9121 attaaaggta agaagtatgt ttcaagtcca tatgttgatg aagtaaataa gtctataaag
     9181 aagattgcta tttcagtacc attattaaat aatgataaag tagttggagt tttatattgt
     9241 acttacaata tcaatactct gatgaaactt attaatatat ctttttatga aaataatagt
     9301 atatcttatg ttgtaaaaaa tgatggtaca attatccttc atccacaagg ggatagccta
     9361 tcaaaaaata tttataaatt gttagaacag gataatgata ttcatgaagt aaatagatta
     9421 aaaaaagaat tgcaagaaaa taaaacaggt gcaactgtat taaatatgtt ggaagaaaga
     9481 agatatttag gatatgccac tatggataat gggaatagtg aaaataatta tataaaagac
     9541 tggaatttaa ttttttcaat tccagaaact gttgtattta gtaattcaaa acaaataatc
     9601 aacagagctg tatatgcagt gttatctatt gtattaatat ttgttgcaat tattttttat
     9661 ataatttata taaaaaatct aatgaaaaag aaatattaag tttggcttat gaagataaag
     9721 ttacatatat aggaaatcaa aataaatttt atagagagtg tagtaagtat cttttagata
     9781 aaccatcttt aaattatatt atagtatatt ttgatattaa taactttaag atgattaatg
     9841 atacttttgg atatgaattt ggtgacaatt tattaattac aatagcaaaa gctttaaaag
     9901 aagaattaac agaaggcgag gtatatgcac gactttcaag tgattatttt gcaattttct
     9961 gtgattataa aaatggaaga aataaaatta taagaaaact tgataatata cgaagtaata
    10021 ttgaaagtaa tttgagtata gtctttgaaa tctctctgtg tgtgggtatt tattttgttg
    10081 aagaaggtga agtagatatt caaaaggctg taaataaagc aaatatggct aggtctgttg
    10141 ctaaaggtaa aaatataaat tatgctattt ataatgaaga tgttaggaat aaacttagtg
    10201 aagaatcaat gattttagac gatataaaaa tagcattggt aaaaaatcaa tttgaagtgt
    10261 attatcaacc taagttttca cttgtaactg gagaaatgat agggagtgaa gcactaataa
    10321 gatggaatca tccagaacat ggttttataa gtcccgcagt atttattcca atagcagaga
    10381 aaagtaagtt gattttaaaa attgggagat ttgtatttga aagggtttgc aatgatttat
    10441 ctgaatggaa aaaacaaggt aaaaagatag ttccagtttc agtaaattta tctagagtcg
    10501 agttgtatca accagatatt gtaaaattta tcaataaaac aatacaaatg tataatctaa
    10561 gttcagattt cattgaaata gagataacag aaactgtggc tattaatgag ttaaatatat
    10621 taaagaatgt tttaaatgaa ttgaggaagc atggattttc tatttctatg gatgactttg
    10681 gcacaggata ttcttctatt agttgtttaa gagatatgcc aattgatata ttgaaactag
    10741 ataagtcttt tcttggtggc attgaacatg atgagagaag tcgcaatata gcaaaatcaa
    10801 tagtgtctct agcaaaatca ctagatttag ttgtaattat agaaggtgtt gagagtaaag
    10861 aacaagctga gttaatgaaa caatttggat gtgatttagt tcaaggattt tattttgcac
    10921 gaccaatgcc agctaagaat tttttagatt tactttaaaa caaaatttga tttttatata
    10981 aagcactatt atgaatgctt gattttaata aatcattgct aaataattag tgttttttta
    11041 ttttatcaat gtattgtcta atataaagca aaaaataatt gtattttata aaaattatat
    11101 taaaaataaa tttattttgc taataaacat gttattaaca aaagaatagt actataatgt
    11161 atagatgata aaaactttaa taaaaattta gtgtatagga gttgtttaag tatgatagat
    11221 gtaagtattg atgacaaact taaggaaaga tggcctaatg ctaagctggg ttgcattcaa
    11281 gcaaaagtag cagtaaataa aagctcagaa aaattaatta atgaaataaa tgagttttgt
    11341 gatactataa atcaaagtct aaaagttgaa gacattacga aactagataa aattaaagat
    11401 gcaagaaatg cttataaaga gcttggaaaa aatccaagta gatatagaac atcttcggaa
    11461 gcattggtta gaagaattat tcaagggaag ggtctttata caataaataa tatagtagaa
    11521 ataaacaatt taatatcaat aaaatcatta tatccagtag gtacatataa tgtaagcaaa
    11581 ttacattcac cagtatgttt tactgtagga gatgaaggag aacagtataa gggtattggg
    11641 aaagaattaa taaatataga aaatcttcct atattgtctg attcagtggg taaatttggg
    11701 agtccaacta gtgattctga aagagcaatg ataacagaag atgcaaatga aatattaatt
    11761 tgtatatttt cttttagtca agaatctgat attgaagaat attttggata tgctaaggaa
    11821 ttacttaaag aatatgcaga tggaaaagat atagaaacta aaataattaa atagaatcta
    11881 tataacaacg ttatgttaag ctaaaattca atataaaaat aagttaagat aaagaaacta
    11941 acacaaaatt agtttctttt ttaaaagagt agtttagtgt tacaaaaata gatagatagt
    12001 cccacgatta ttaaaagtat ggtaatatat taatatagca gagattgtgt ttaagggatt
    12061 ttgaaagggg ggatttataa taatcatttt atctaaaaga taaaatggtt ttatggaaat
    12121 aactatatat ttaaaaatat aaataatgat acagtaattt gggtatataa aataaggcat
    12181 acatggtttt gtttaaaaac caataaggta cataggtaat tacaatgtaa ggaaggattt
    12241 aattatgatt gatggaaaag aggtaaaaca agaaagtgga caaaaaaatc taaaatttaa
    12301 gatagctata gtagtaggtg ttttgtttgt agcttatata ggaatggcaa tcttttttag
    12361 aaatcatttt tactttggaa cgactattaa tggcgtaaag gcttcaggaa aaacagttga
    12421 gcaagtaaat gatttgttaa catctagtac taattcctat actttaaact tagaggaaag
    12481 aaacaataag aaagaacaaa taaaagcaaa agatattgat ttaaaattta caccagatga
    12541 taaagttcaa aagataaaag atagtcagag cgctattgga tggattggtg gaatatttaa
    12601 gtctaaagag tataataata tgataactct ttcatataat aaagatttac tagaaaaaag
    12661 gtttaatgct ttatcttgtt ttgatagtaa aaatgttgta gaacctaaga gtgcttatcc
    12721 taagtatgat agcactaaaa acgcttatgt tatagtacca gaggtacttg gaaacaaagt
    12781 gaaaaaagat gatttttata agttagtaca aaactcaatt aattctggga atacttctat
    12841 ttctctggat gaaagtaaag tttatgaagc accaaacaac acaaaggatt cagaaaagtt
    12901 gaaacaagct attaagacat tagataagta tgttggtgta gtcgttacat atgattttag
    12961 tgaccgtaag gaaactttag atggttctga aattaataaa tggttaaaag tagactcaga
    13021 aaagaattat gacataactt ttgataatga tgctatggta gaatatacaa gaggtttaag
    13081 taaaaaatac agtacttttg gtgactcaag accatttaaa actgccacag gtaaagatat
    13141 tacaatttca ggtggaattt atggttggtt gatagataaa aagaaagaag ctgaagcatt
    13201 agttgatgtt gtaaaagctg gtaaaaatgt tacaagagaa cctatttaca tacaaaaagc
    13261 tgtatctcgt aataaagatg atattggaaa tacatatgta gagattagtc tttctggcca
    13321 acatatgtgg ttctttaaaa atggagaaat gcttgtatca acagacgttg taacaggtga
    13381 tttagctaga ggatttgcta caccatcagg tatatatcct cttaattata aggctagaaa
    13441 tgtttcatta actggtcaag gttacagttc acctgtaagt tattggcttc catttaatgg
    13501 gaacataggc attcatgatg ctacttggag aaattctttt ggtggaagta tatataagtc
    13561 ttcaggttca catggatgtg taaatacacc tttttctaag gcaaagaaaa tatatgaaaa
    13621 tatagaacca ggtacaccaa ttattttata ctagtaaatg tgttgctaac agttaagcga
    13681 agtcatctta gtttatttag gatggcttcc tttttatata aagcttattg aagtttacag
    13741 aaagactact taaaaggaga aatacataat gaatgaaaaa ttaactaaaa ttttagaaga
    13801 agctaagaat gatgatgaat ttagaaagga acttataaaa acaagagaac aacgtgatgt
    13861 tatggattca ttttgtaagg ttgttacatc tagaggttat gatatgacag taggggaact
    13921 tttttctatg ggagaagaat atacatccaa tctactgaaa agtgtaaatg gaggagcagc
    13981 atatcctatt gaagattgga gtgattggta cgaggatttt tttataatgt tgtcgaatat
    14041 atagagtaac taatgatatt atctacttaa gattaataca ttgccttaaa ataaaactat
    14101 tagaactagg tgtaagtttt atcaatcttt aaaaatattg atttatttct aactagatat
    14161 aaaaaaatta gattaaaaat aaggtgacaa aaatcgatat agcttgatga tttataaaag
    14221 ttagataaaa tagtgcgaga tatttctaaa aattcaaaaa aaacaataaa cttcaatggt
    14281 tagaggttga aaaagaatat aaaaaacttt tatatgtaat aaattaaatt aaaattataa
    14341 aaaaaataaa ataaaaattt aaaatgtcga cataacgtta attttaaaac aaaataacgc
    14401 tattgacaaa ctatatcgct ttaaattata attattgaaa ataatggtat ttaaaaacca
    14461 tactcatgtc atagtctaaa agtaagtcaa aaaaggatgt aaatctaggc aagtactatt
    14521 ctaaaatatg ttttattaag aaataaacgt tgaaaaatat taattattat ataaaaatgc
    14581 tgtttatact ttaacaatga ggtagtaatc taattagaat aaaaaaatct aattggttat
    14641 atacatagta aaaaggtaag agaggtgatt tggatggctt ttgaaataat aaaaagcatt
    14701 gttgaggcag agcagacagc agacagtatc aaagtaaaag ctgttactga tgcagagtct
    14761 atcagagctg atgctgtaaa caaatgtgaa agcatatttg ctgatgtaaa aaaacaagca
    14821 aagcttatgg aagaaactct tattgagaag gcagtcaccg acagtagagc agaggttgat
    14881 aaaatcttag ctaatgctaa aagtgaatgt ctgaaaattg aaaaaactgc tgaagaaaga
    14941 aaaagtaagg ctattgaagc ggttattgga aaggtagtga gataatatgg caatagttaa
    15001 tatgaacaaa atatctattg ttggcttaga agctcaaaaa agccagattt taaagctact
    15061 tatgaaccgt gggtttgttc agattgatga tagtgccttt cttactgagg aggaggaatt
    15121 aaaaaattat cttgtaaaag atagtgaaga atctgaagtt ataatgctag agcagaaaat
    15181 gtctcatgta aaccaagcaa ttaatattgt ttcttcacta gtgaaacaaa aaaaatccct
    15241 acttgcacca aaaagagagt ttaacaaaat tgatagtgaa aaagaagggc aagtttatgc
    15301 tgaagctctg gagttaaaca atttgcacaa ggagataggt acaataaggt ctaacataaa
    15361 cttacttaac aacaataaaa gtatgttaga accatgggtg aactttgata ttcctattga
    15421 aaatatggaa actaagtata caaaaactat acttggaact gtgcccataa gcgcaaacat
    15481 tgatgcttta agagtaaaat tagaagatga aataccagaa agtgttattg ggattgtcaa
    15541 ttctgacaag ttaatgaact atgtttattt aattacttta aaagaaactt ttgatgatgt
    15601 tttagaagtt ttaaaagaat ttaatttttc agtggtttca ttaccaaatg aagaaggtac
    15661 accgttacaa gctatcaaga aatacaatga aatgataatt agacaggaaa acgtttctaa
    15721 ggacaaaaca gaggaaatta aaagacatgc agatagtata cacatgcttg aaaacctata
    15781 tgatttttac actatagaaa gagaccaaaa aaagattata gagagacttg tcaaaacaaa
    15841 aacaacattc tgtcttaatg gttggttacc aagcaaaagg gcagatgaat taattaaaga
    15901 aattacagaa aagtttgatt gttgtgttca gactgaagaa ggaagtaaag aagaaggctt
    15961 cccaattctg cttgaaaaca ataaattagt aacacctttt gaaagtgtaa ctaacatgta
    16021 cagctatcca agtacaaaag atattgaccc aaatacaatt ttaactttct tctttgttgt
    16081 attctttgga atgatgctaa gtgacgccgc atatgggtta ataattgcag tagtttgtgg
    16141 atttgttgta tataaattaa agatacaaaa aggtgaagga aatttaatta aacttattgg
    16201 tatctgtgga gtttcaacta ctgtatgggg tcttatattc gggggtattt tgggggattt
    16261 aattcctata aaagcattaa ttaatccttt agaggatgtt atgacactta tgggaatgtc
    16321 gttactgttc ggtattatcc atatatatgt aggtttggga ataaaagcat atacattaat
    16381 aagaagtgga aaggtaatag atgcaatctt tgacataggt ttttggtatt tgtgtataac
    16441 aggtgtatgt ctacttataa taccttttgt tgcaggggac ataggtgtat tttcagaagt
    16501 aggaaaatat ttagcaatta taggtgctat aggtcttgta cttactcaag gaagaagctt
    16561 taaaggtatt cctgttaagc tttttaaagg attttcaagt ctctatggta taacaagtta
    16621 ttttgctgat atattatctt atacaagaat tatggcgctt tgtttaacaa caggagttat
    16681 agcacaagtt atcaaccttt taggagctat agctggtccg atacttgcag ttgtaattgg
    16741 tgttgtagga cacacaataa accttcttat aaatgcacta ggtgcatatg ttcatacaag
    16801 taggttacag tatgtagaat ttttcaataa gttttatgaa ggcggaggag ttccttttgt
    16861 tccgtttaag tataaaacaa aatatacaag cattaataaa aaggagatgt aaatatggaa
    16921 tttttaaacg cgttaggaaa tggtcaattt ttggcaatat caggagctgc tatagcagca
    16981 ttatttgcag gtatgggttc tgctaaaggt acttcaatag caggtcaagc cgcagcaggt
    17041 gttgtaacag aagaccctag taaattcggt caacttctag ttttacaact tcttcctggt
    17101 acacaaggtc tgtatggatt tatcatagct ttccttattt tatcaaaagc aggaattatt
    17161 ggtggagatg caataccaac atcagctcaa ggtttacaac ttcttatggc aggacttcca
    17221 attggtttag ttggtttagt atcaggtata gcacaaggta aagcagcagc agcaggagtt
    17281 ggaatagttg caaaaagacc tgaagaactt ggtaaagcta taacatttgc agcaatagtt
    17341 gaaacatatg ctcttttagg actacttgtt tctttcttag cttacaatgg aattgcaatt
    17401 ggttaattat aaattataat tcataagtaa aaaaaatgaa aaggaggaag cactcctatg
    17461 ggtaatgaac aaaaaatgat tgacagaatt atagctgatg ccaaacaaga agcacaagaa
    17521 atattggata aagctaaaag tgaagcagat ttaaaagtaa attcagctaa tgaaaaagct
    17581 gaaaaggaaa tggcttccta tacaaagctt gcagaggcag aagcagaaaa agcggcttct
    17641 aaggaaatat caggagctta tatggaggct aaaaagcaaa tattatcaaa aaaacaagag
    17701 attttagaag aggtaatttt agaagctaaa aacaaacttt taaatcttaa agataatgaa
    17761 tatgaagaga ttatactaaa catgattgaa aaatcaaatt gtacagatga ttcagagata
    17821 gtattatcta aaaaagataa aaaaacatta aaagatgtgc tatcaaaaaa aggcattaaa
    17881 gtgtctgatg aaactagaga tattactgga ggtttcatag ttaaaaaagg tgacattgag
    17941 tacaattatt catttgaagc cataattgca gtagagcatg aatatattga acaaattgca
    18001 gcagaaattt tgtttaatta aggaggggtt aaaaaatggc agaagaaaaa acttatccct
    18061 gtgccgttgc acgaataaga gtgcttgaaa aacagcttct taacagacaa atgtttattc
    18121 aaatggcaga agccaaaagc cctgaagact ctttaagaat tatagctgag gcaggttatg
    18181 ctgatacatc aaataataat atacataatt ttgagaatat tttgactcaa gaattatcaa
    18241 agacatatga aattcttaaa agtctagctc ctgaagaaaa atttgttgat gtatttcttt
    18301 ataaaaatga ctatcacaat ttaaaagtgc tgattaaaga agaaatttct ggtgtagatg
    18361 gtgagaaata tttaattgat ggaggtacaa ttcctcttat taagcttaga gaatctttag
    18421 caaatcgtag ttttagtgat ttacctaaga taatgtcatc agctatatta gaggcatttg
    18481 atgtttataa taagacacaa aatggccaga tggttgatat tgttctagat aaagcaacct
    18541 ttaccagtat gaaagaaact gctaaagaaa gccaaaacaa atttgtaata gattatgtta
    18601 tgaaagtttg tgatttaaca aatctaaaga gttttataag agtcaaaaat atgaagaaag
    18661 attttgatat gtttatgaat gtgtttgttt caggtggctc acttgataag gaaaaattct
    18721 tagaagcttt ttcctcagat actccagcat catgttttaa atctacttct tactctgatg
    18781 tctgtaaaaa tggcatggat agtggtttta ctgtctttga aaaactttgt gatgactatc
    18841 ttatggaata tgttagagga gcaaaattta agcctcttac attagaacca ctaattgcat
    18901 atttatatgc taaagaatca gagataaaaa caattagaat tattttgact agtaagctta
    18961 ataatataga cgcagatact ataaaagaaa ggctaaggga tgcttatgta taaggtagga
    19021 gtagttggag ataaagatag catcatggga tttttagctc ttggaataga tatcttccca
    19081 gcctatgata gtgacgaaat taaaaaaagt atacataagt tggttgaaga tgaatatgcg
    19141 ataatttata ttacagaaca ggcatcattg cttgcaaaag aatcaatagc aaaatacaaa
    19201 gattaccaac ttcctgccat tatagttatt ccaggtattg gaggaagtat gggattaggt
    19261 atgaatgaag taagagaatc tagtaaacgt gctataggtg cagatatatt atttaaagaa
    19321 tagatgaaag ttgaataaaa gacaatctca aattttattg gtactaaaga tatgtgctaa
    19381 taaaatatag gagggaatat ttaaattttg ataaagtata tgattgcagg tgagagaata
    19441 tgaaaacagg cacaattgta aaggtgtcag gaccgcttgt tgtagcggag ggcatgagag
    19501 atgcgaatat gtttgacgtc gtaagagttt cagataaaca tcttataggc gagattatag
    19561 aaatgcatgg agataaagct tctatacagg tttatgagga aacttctggt ctaggaccag
    19621 gggaagaagt agtttctctt ggaatgccta tgagtgttga acttggacca ggtcttattt
    19681 caactattta tgatggtatt caacgtccac ttgaaaaaat gtatgatatt tcaggtacta
    19741 atataactaa aggtgttgaa gtatcttcac ttgatagaac aactaagtgg gaatttaaac
    19801 ctaaaaaatc tgttggtgat aaggttgtag caggagatat aattggtggc gttcaagaaa
    19861 cagcagtagt agaatgtaaa attatggttc cttatggagt taagggtact ataaaagaaa
    19921 tatattctgg tgaatttact gttgaagata cagtttgtgt tattacagat gaaaaaggta
    19981 atgatatacc tgtaactatg atgcaaaaat ggccagttag aaaagaaaga ccatatagag
    20041 aaaagaaaac accagattca cttcttgtta caggtcaaag agttatagat acatttttcc
    20101 caatcacaaa aggtggggta gcagctatac cgggaccttt tggaagtgga aaaactgtta
    20161 cacagcatca gcttgctaaa tgggcagatg cagatatagt tgtatatata ggctgtggcg
    20221 agcgtggaaa tgaaatgaca gatgttcttc ttgaatttcc tgaattaaaa gacccaagaa
    20281 caggcgagtc acttatgcaa agaactgtgc ttatagcaaa tacatcagat atgccggttg
    20341 ctgcacgtga agcttctata tacactggta ttacaatagc tgaatatttt agagatatgg
    20401 gatatagtgt tgcacttatg gcagactcta catcaagatg ggctgaggct cttagagaga
    20461 tgagtggtcg tttagaggag atgcctggtg aagaaggtta tcctgcatac ttaggttcac
    20521 gtcttgctca attctatgag agagcaggaa aggtaaattg tctaggtatg gatgaaagag
    20581 aaggaacact tacagcaatt ggtgcagttt ctcctcctgg tggtgataca tcagaacctg
    20641 tcagccaagc aacgcttcgt atagtaaaag tattctgggg tcttgatgca tctttagcat
    20701 ataaacgtca tttcccagca attaactggt tgactagtta ttcactctat caggaaaaag
    20761 ttgatgtatg ggcagataaa aatgtaaatg ataatttctc agctcaaaga gctcaagcta
    20821 tgaaactttt acaagttgaa tctgaacttc aagaaatagt tcaattggtt ggggttgatt
    20881 cactttcaga tggagataga ttgatactag aggttactcg ttctataaga gaagacttcc
    20941 ttcatcaaaa ctctttccat gaagttgata cttatacatc acttcataaa caatatagaa
    21001 tgatgggact tatattaaaa ttctataagt cagcacaaga tgctatcaag caaaatgtta
    21061 ctataaacga tattatcaag ctttcagttc ttgaaaagat tggtcgtgct aagtatgtag
    21121 aagaaggcga tatagatgca gcttacgatt caattgaaga tgaaatagac aacgagcttg
    21181 cagaccttat taaaaagagg ggggctgaag aactatgata aaggaatata agtcaataca
    21241 agaggtagcc ggtcctctta tgttaataaa cggcgtagaa ggtgtaaaat ttgatgaact
    21301 tggagaaatt gaactttcaa atggagaaat tcgccgttgc aaagttctag aggtaaatgg
    21361 agatactgca cttgttcagc ttttcgaaag ctcaacaggt ataaaccttg ctgaaagtaa
    21421 agttcgtttt ttaggaaaga gtcttgactt tggagtttca ccagatatat taggtcgtgt
    21481 ttttagtgga atgggtagac ctattgatgg aggtccagaa attataccag ataaaagact
    21541 tgatataaat ggagctccaa taaatccagc agcacgtgat tatccggccg agtttataca
    21601 aactggagtc tctgctatag atggacttaa tacattggtt agagggcaaa aacttcctat
    21661 attctcaggg tctggtttgc cacatgccaa tcttgcagtt cagattgcaa gacaggcaaa
    21721 agtacgtgga actgattcta agttcgccgt tgtatttgct gcagttggta taacttttga
    21781 agatgctgaa ttttttataa gcgattttaa agcaactggt gcaatcgacc gttctgtagt
    21841 gtttgtaaac cttgcaaatg acccagctgt agagcgtgta tctacaccta gaatggcact
    21901 tacagcagca gaataccttg ctttcgagca agatatgcat gtacttgtta taataacgga
    21961 tattacaaac tatgcagagg cattgcgtga agtttcagca gctaaaaaag aagttccagg
    22021 acgtcgtgga tatccgggat atctttatac tgaccttgct actatgtatg aaagagcagg
    22081 aaaaatgaaa gggcatgaag ggtctataac tatgattcca atacttacaa tgcctgagga
    22141 tgataagaca catccaattc ctgaccttac aggatacatt acagaagggc aaattatact
    22201 tagccgtgaa ctttataaaa aagatataca gcctcctata gatgttttac catcactttc
    22261 tcgtctaaaa gataaaggta ttggtaaagg taagactcgt gaagaccatg cagatacaat
    22321 gaaccagctt tttgcagctt attcaagagg taaggatgca aaagaactta tggctatact
    22381 tggtgaatct gcactttctg agattgattt aatgtatgct aagtttgcag atgaatttga
    22441 gaaagaatat gtttcacaag gatttagaac tgatagaact attgagcaga cattagaaat
    22501 cggatggaag cttcttagta tgttaccaaa aagtgaactt aaacgtatcc gtgatgaata
    22561 ttttgaaaaa tatatgccta aggagtgata tctgtggcta gattaaatat aaatccaaca
    22621 cgtatggaaa tgactagact taagaaactc cttaaaactg ctacaagagg tcataagctt
    22681 cttaaagata aacttgatga acttatgaaa caatttcttg agattgtaag agaaaataag
    22741 cgattgcgtg aagaagcaga aaatgctctt gatactgctt ataaaaattt tataatcgca
    22801 agagcagtta tgagtcagga gtatctgggt tcagcactta tgatgccaaa gcaatcagtt
    22861 tcagtagatg tttctacgag aaatataatg agtgttgatg ttcctgtgtt tgactttaaa
    22921 actgaaaaca atcaaagtga tatttatcct tatggtcttg cttttacatc aggtgaatta
    22981 gattctgcta tggaagcttt ctcagatgct atgcaaccac ttttaagact tgcagaaagt
    23041 gaaaaaagtg cacagcttct tgcacaagag atagaaaaga cacgtagacg tgttaatgct
    23101 cttgaaaatg taatgatacc aaattacatt gagactatta agtatatagc tatgaagctt
    23161 gaagaaaatg agagagcaag tactactaga cttatgaagg ttaaggatat ggtgcttaaa
    23221 aaagcacttg aagaaaagaa aaaaaatgat ttggttgtat aattaaatac ttataaaaat
    23281 aagtctaaca ttctacttta tagaggttag acttattttt tatgtttaaa ttaaaatgta
    23341 gatactataa aaattaatat attgtttaaa aacagactgt atttaactaa aaataatata
    23401 tttaacaaaa atttctaaaa attaacgaac tgttaattta cacttaactt tatattaaca
    23461 aattaggtgt attatataaa cataagcaaa aacaataaaa aacaaataaa aaaaggaaaa
    23521 aacacaaagg gggaagatat gaaagaattt atatttactt gtctgtgcat tgtagtgatt
    23581 acatttatga caagttgcat atcaacaaca ccacaaacta ctcaaggaaa aggaaataat
    23641 aaacttacaa tatatgcagc actagaggaa gagcagatag cttattatat ggaaggtttt
    23701 agagaagagt atcccaatat agatgtagag atagttatgg attctcatgg tgtaatagct
    23761 tcaaaactat tagcagaaaa agataatcca caagcagata ttatatgggg attatcagca
    23821 atgaacatga tagagctaaa aaaagatggt gcacttgaag catataagcc taaaaattat
    23881 gataaagttg ataaaaaatt taaagatact gaagatgatg tatcttgggt aggaatgagt
    23941 gtcccagaga cagcaatagt agttaattat aaggaactta agaaactagg tatagatatt
    24001 ccaaaaagtt atgaagattt aataaaacca gaatataaag gacttataac aatgccaaat
    24061 ccagcatcat ctggaacggg gtatcttaca gtatcagggc ttattcaaat gatgggtgaa
    24121 aaagatgctt acaattacat ggataaatta cataaaaata tagccacata tacacactcg
    24181 ggttcaaaac ctgcgaagtt agctggctct ggagagtatc caataggtat tacacttgga
    24241 tatagagcta cacaacaatt atctagtgga gaacctatag aggttgtatt tccaaaggaa
    24301 ggttcaggtt gggacttaga agcaaatgcc ttagttaaaa aagataatat aaaaaaagaa
    24361 gcaaagctat ttttagattg ggcaataagt gattcagcta tggatagata tgcacatgaa
    24421 gtagcagtta cttccataaa gacagataat ccagttccaa aaggttattc taaacaacca
    24481 tttgaacagc ttataaaaga agttgattta aactctgctg ccaaaaacag aagtacagta
    24541 cttaaaactt gggaatcaaa atacggttca aaatctgaaa gtaagtaaag tttaaattaa
    24601 taatttagat gaaagaattt tagaagaaaa agtaggatat aaaatttaaa ataaaaaaat
    24661 aactatataa ggagagataa gatatgagtt acttaaaaat aaataatgtg tttaaaagtt
    24721 atgaccaaaa aaaggtttta aataatatta gtttggatat tgaagaaggg gaatttctat
    24781 gtttactcgg acctagtgga tgtggtaaaa ctacattact tagaataata gcaggtcttg
    24841 aagatgtaaa tagtggaact ataattctcc aggataaaga tattacaaat ttagagccat
    24901 caaagagagg ttttggtata gtatttcaat catatgcatt atttccgaat atgactgctt
    24961 ataacaatat agcttttcca ctaaaagaga gaaaagtttc aaaagaaaaa atagataata
    25021 aagttaagga agtacttgaa acagttggat tgactaatga agctcataaa taccctaaag
    25081 cattatcagg tgggcaacaa cagaggattg caatagcaag agcattagct cttgaaccaa
    25141 agtttttact tttagatgaa ccaatgtccg cattagatgc aaaggttaga cacaagctta
    25201 gaatggacat taaaagatta caaaaagaac ttaatataac gacaataatg gtaacacatg
    25261 accaagaaga agctataact atggcagata aaatagctat tctaaatgga ggagatataa
    25321 tgcaaatagg gactccagaa gaaatatacc aaaatcctca aaacctattt acagctcaat
    25381 ttataggaga tacaaactgt tttgataatg gagattctat attaactgta agaccagaat
    25441 atgttcaaat agagaaatcg acaaaagaaa actaccaagg gataatatca aatatagagt
    25501 ttagagggaa tttattgaga gttgaaataa aagataaatt aaatgaaaat ttcataataa
    25561 gtgatgtttc tataaaagag tgggttaatt taaatttagt agaaggtgac cttgtaaaaa
    25621 taagtataga cgaaaaatac tatttaaagt atccaaaagt taaaggagca taagcgtatg
    25681 aataataatg ctataagttc taaaagtata agtcttaaat caaaaatgaa tttagatata
    25741 aaaaaatgta tgcttacagt attagttatg tctttagtaa tatttttatt agcacctata
    25801 atatatctat ttgtacaggc ttttttagat aaagatggag tctttgtagg attacaaaac
    25861 tttcaagaat acttggcttc tgaatctcta ttaaattctc taaaaaatac agtatttgta
    25921 tcaattatat caactgtaat atcactaaca cttgcattag tatatgcata tgcattaact
    25981 agaacaaaga ttaaatttaa aggttttttt aaatcattag ctatgtttcc tatgtttgct
    26041 ccaacaatga tgcatgcaat aggtctagta tatttatttg gaaatcaagg tttggtaact
    26101 aatttacttg gagtgcatgt ttcaatatat ggagcagtgg gaataatatt ggcacaaatt
    26161 atatatgtat ttcctcaatc atttcaaata ctttatatat ctctaagttc aaccgattat
    26221 agactatatg aagctagtaa tactatgggg ataagcaaga taagagaatt tataagcata
    26281 acaatcccaa gtataaaata ctctttaata agttctgcat tcgtatcgtt tacattatgt
    26341 tttacagatt ttggagctcc aaaggtgata ggaggagagt ttaatgtatt atcaatagat
    26401 gtatataagc aaattatagg gcaacagaat atgccaatgg gagcaactgt atcaatatta
    26461 cttttaatac ctgcaataat atcgtttata gtaactaaaa aaattgaaaa aaatcaaaat
    26521 tcatatataa cttcaaagtc aattccatat aaggttaaaa ctaataagtt aagagatagt
    26581 attttaggag gggttgtagg aattattgca tttggtataa tagctgtaat gttaactgta
    26641 atcttatctt cattaatatc agtttggcca tatgatttaa gactgacact ggaacatttt
    26701 tctatgaaaa acagtattga tgggattaaa ccatttataa attcaataaa aatgtcacta
    26761 ttgacagcta ttctagggac gagctttgtt ttcttatttg catatttgaa tgaaaaaaca
    26821 gaaaaagctc catatttaaa atcatttggg tattttttaa gtacattgcc tatggcattg
    26881 ccaggattag ttttaggtat agcttatgta ttatttttta ataaattaga gtttaatttt
    26941 atggatacaa tatacattaa gaatttgttt aatggattat atggaacatt acttataatg
    27001 gttatatgta atatagttca tttcttttca gtagcattta taacagctac tactgctatt
    27061 aagaaagtag ataaagaatt ggatatggta gctaaatcaa tgggaataaa cagcgcatca
    27121 attcttaaga acgtaactat accactttgt ttgccagcag ttttagagaa ttttatgtac
    27181 tttttcctaa actccatggt gacaatatca gcattagttt ttatatatac tgcaagttca
    27241 aaagtagctg caatatcaat tgtacaacta gatgataagg ggagtactgc acaggcagca
    27301 gcactagctg tgttaatatt agtcacaaat atattagtga aagtaatata tgaatttata
    27361 aagaatcgtt tagaaaaaag aacaaacttt aattaaataa tttatcttat aaaaacacaa
    27421 atttaaaaaa gtctattctt atgatttatt atttaaagaa tagacttttt aaatttatta
    27481 ttgatattgt ttattaattt tccttttgtt atcatacatt tgagaatctg cacgacgata
    27541 tgtatcaaat aaagaaacat ctactttttc atcaaaaata gcatatccaa tagacattga
    27601 taattttaat tcattagttg agttttcttc aactattttt aaattaatat tatttataat
    27661 attatttatt tctttgtgag ataaattttt tagaataatt atgtattcat cacctccaat
    27721 acgataaact atatattttt tatttattac atattgtata attttacttg cttgcttaat
    27781 atataagtct cctgtctgat ggccaaattt atcattaatc ttttttaaac cgtctaaatc
    27841 tattgatatg atagatatta gattctttag atttgaattg tttaagttat tcatatctat
    27901 ttcaaaagca tttctatttt ttaggcctgt taaataatct gtattagcaa aatcagtata
    27961 tgtaggatta gatattcttt taaatagaat cactgcaatc aatgctgata ttagacaagt
    28021 taataatgct attattggtg taactatact tataaattta aatgtattgt attgatgttt
    28081 tgcttcaaat tcaattccta ttactccaac aaccttacca acatcattgt gaataggaaa
    28141 atacgatata aatatgtatc cccaagatgt tgatttaatc tttttaggta acaatatatg
    28201 attatctaat gctttgttaa ggtctcccca aatctctttt tctatcaaat caccagcatt
    28261 tctaaaatca ctcctagatg aatttaaacc atcaacaaca tatactaatg atccatcttt
    28321 tgttttttta gcagtatata gatacatgac tccagtagta ttttttacag attctaaaag
    28381 gattttagaa cttttataag acttttttga ttggtcttct ttagaattaa tttcagtaaa
    28441 ggtattttta tcaatatttc tttctacata ttcatagata tgttttgacc tctcacttaa
    28501 agagtatatc atgctcttat aagttagtgt ataattaaaa aaatatacac atgaggaaga
    28561 tattattaca ataattgcag taagtattga aacctgcata tcaactctat tgaatttttt
    28621 atttattttc atagcttaaa tgtcttgtga aaagaacctc ccacaatctc cccataatta
    28681 atatatatct ataaatttca ccataatata ttcatatatt atc
//
LOCUS       DAEMHZ010000042        27917 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000042 DAEMHZ010000000
VERSION     DAEMHZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.042"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..505
                     /locus_tag="KPK72_001707"
     CDS             122..505
                     /locus_tag="KPK72_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2488933.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            507..857
                     /locus_tag="KPK72_001708"
     CDS             507..857
                     /locus_tag="KPK72_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488934.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYSIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(958..1416)
                     /locus_tag="KPK72_001709"
     CDS             complement(958..1416)
                     /locus_tag="KPK72_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488935.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLKEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1461..2249)
                     /locus_tag="KPK72_001710"
     CDS             complement(1461..2249)
                     /locus_tag="KPK72_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2488936.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2388..2825)
                     /locus_tag="KPK72_001711"
     CDS             complement(2388..2825)
                     /locus_tag="KPK72_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2488937.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3647..3781
                     /locus_tag="KPK72_001712"
     CDS             3647..3781
                     /locus_tag="KPK72_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2488938.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGVSQTKEY
                     "
     gene            3905..4750
                     /locus_tag="KPK72_001713"
     CDS             3905..4750
                     /locus_tag="KPK72_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HBG2488939.1"
                     /translation="MSNIINAIYNLINNPVIELREFYKSKNRANNMGDALEEYVKDLF
                     ANTINEQNEQSRLKKISDTFSYLGNQNNPPDSILHLGDAIEVKKIEGKNASLALNSSY
                     PKHKLYANSKLITEACRKCETWSEKDIIYAVGVVNGSMLNSLCMVYGIDYAADNSTYE
                     KIKEIIVSGVNNIPDVEFAPTNEIAKVKKVDPLGITDLRVRGMWSIENPFKVFNYIYR
                     RDFDKKFNFMCVIRETKYSSFENKSLIENCVGSIQGFNIVDVKIKNPNNPALLENAKL
                     ITFQI"
     gene            4773..5756
                     /locus_tag="KPK72_001714"
     CDS             4773..5756
                     /locus_tag="KPK72_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2488940.1"
                     /translation="MKLVSLFSGAGGLDLGFEKAGFEIIMANEIDKTIWDTYEKNHTA
                     KLLKGDISKIASREFPECDGIIGGPPCQSWSEAGSLRGINDPRGQLFYEYIRILKDKQ
                     PKFFLAENVKGMMAKRHNEAVTNIIQQFEEAGYNVFIKLLNAVDYGVAQDRKRVFYIG
                     FRKDLNINFEFPPAFNEKKCFKDIIFDLKDNALPALEKNKTNGMSCKVLNHEYFTGAY
                     STIFMSRNRVRSWEEPAFTVQASGRQCQLHPQAPKMIKFGKNDCRFVEGQENLYRRLS
                     VREAARVQGFPDDFEFVYTSLNDGYKMIGNAVPVNLAYILAKQIKKYLENN"
     gene            6102..7197
                     /locus_tag="KPK72_001715"
                     /pseudo
     CDS             6102..7197
                     /locus_tag="KPK72_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            7175..7318
                     /locus_tag="KPK72_001716"
     CDS             7175..7318
                     /locus_tag="KPK72_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488941.1"
                     /translation="MKMTVFFSFFEKSATKHQIAYRYGARKENSTFLKNGYYLRLCCT
                     DMR"
     gene            7932..8153
                     /locus_tag="KPK72_001717"
     CDS             7932..8153
                     /locus_tag="KPK72_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBG2488942.1"
                     /translation="MTKAVELLVKKYHRKRACAIDNPVKTMQKKSKRSIGNRGKTKIV
                     VKEHFSKKGKTMEELLTDVMLEKAKQTIA"
     gene            8265..9532
                     /locus_tag="KPK72_001718"
                     /pseudo
     CDS             8265..9532
                     /locus_tag="KPK72_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            9569..9967
                     /locus_tag="KPK72_001719"
     CDS             9569..9967
                     /locus_tag="KPK72_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2488943.1"
                     /translation="MSVPDSDMLISISEVLETSVSTLLGETIVESEVDNLKVISEKLE
                     IINLQLAQRKNMRRKMFHWLFILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVA
                     FHAFEWLFVRLVPIVLIGAIIGIFVTRKKI"
     gene            9994..10143
                     /locus_tag="KPK72_001720"
                     /pseudo
     CDS             9994..10143
                     /locus_tag="KPK72_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            10372..10908
                     /locus_tag="KPK72_001721"
     CDS             10372..10908
                     /locus_tag="KPK72_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2488944.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKR"
     gene            complement(11342..12142)
                     /locus_tag="KPK72_001722"
     CDS             complement(11342..12142)
                     /locus_tag="KPK72_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2488945.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12132..12884)
                     /locus_tag="KPK72_001723"
     CDS             complement(12132..12884)
                     /locus_tag="KPK72_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2488946.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEAISISDKVAILSKRPATIKNIYDIELN
                     MDGTKTPLKTRKVPDFQKYFDILWKELDTHEA"
     gene            13054..14412
                     /locus_tag="KPK72_001724"
     CDS             13054..14412
                     /locus_tag="KPK72_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488947.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            14405..15037
                     /locus_tag="KPK72_001725"
     CDS             14405..15037
                     /locus_tag="KPK72_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2488948.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            15366..16523
                     /locus_tag="KPK72_001726"
     CDS             15366..16523
                     /locus_tag="KPK72_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2488949.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(16670..17305)
                     /gene="lexA"
                     /locus_tag="KPK72_001727"
     CDS             complement(16670..17305)
                     /gene="lexA"
                     /locus_tag="KPK72_001727"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG2488950.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(17409..18536)
                     /locus_tag="KPK72_001728"
     CDS             complement(17409..18536)
                     /locus_tag="KPK72_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2488951.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(18857..19702)
                     /locus_tag="KPK72_001729"
     CDS             complement(18857..19702)
                     /locus_tag="KPK72_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488952.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILDDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(19732..20103)
                     /locus_tag="KPK72_001730"
     CDS             complement(19732..20103)
                     /locus_tag="KPK72_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2488953.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(20256..20516)
                     /locus_tag="KPK72_001731"
     CDS             complement(20256..20516)
                     /locus_tag="KPK72_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG2488954.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(20705..21649)
                     /locus_tag="KPK72_001732"
     CDS             complement(20705..21649)
                     /locus_tag="KPK72_001732"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG2488955.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(21670..22524)
                     /locus_tag="KPK72_001733"
     CDS             complement(21670..22524)
                     /locus_tag="KPK72_001733"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG2488956.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(22539..23900)
                     /gene="accC"
                     /locus_tag="KPK72_001734"
     CDS             complement(22539..23900)
                     /gene="accC"
                     /locus_tag="KPK72_001734"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG2488957.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(23916..24368)
                     /gene="accB"
                     /locus_tag="KPK72_001735"
     CDS             complement(23916..24368)
                     /gene="accB"
                     /locus_tag="KPK72_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG2488958.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(24638..25084)
                     /locus_tag="KPK72_001736"
     CDS             complement(24638..25084)
                     /locus_tag="KPK72_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488959.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            25359..25637
                     /locus_tag="KPK72_001737"
     CDS             25359..25637
                     /locus_tag="KPK72_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488960.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(25766..26512)
                     /locus_tag="KPK72_001738"
     CDS             complement(25766..26512)
                     /locus_tag="KPK72_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488961.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(26641..27285)
                     /gene="trmB"
                     /locus_tag="KPK72_001739"
     CDS             complement(26641..27285)
                     /gene="trmB"
                     /locus_tag="KPK72_001739"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG2488962.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(27423..27743)
                     /locus_tag="KPK72_001740"
     CDS             complement(27423..27743)
                     /locus_tag="KPK72_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG2488963.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT         9972 a         4198 c         3953 g         9794 t
ORIGIN      
        1 tgaataggaa gtaattaaaa agaattttta ttgattttta aaaatttagt tagaaactat
       61 atcattatgg taaaaaatgt attataatgt ttagaaatat tataataagg agcgattaaa
      121 catgaatcta tcgaagaaaa tagaagataa aagaaaagct aaacagagag ctgaaaaagt
      181 taagaaagct aaaattgcta cagctggtgt ggtattagga gctgttactg gtgcagtgag
      241 tggagtactt ttggctccta aatctggaaa agaaacaaga gaggatataa aagatgcttc
      301 acaacaaatt gctgaaaaga taaatatgaa aactgtagat gtaaaaggca aagtatctga
      361 gaagctagag gataaaaaag gaaactttat agaatcaaag aaaaaaataa aaaaatatat
      421 agataacaaa aagagtgttg aaaattctag tgaggaagat aaaataatag atatagttga
      481 accagttagt gttactgagg aataatatgg acaatacaat aacgattaac ataagtataa
      541 tatatgtatt attagctata cttgcttgta ttgctttagt ttattcaatt attgtacttg
      601 ttaaactaca taagatttta aataatgttg gaactatgtt aaatgaaaat actcaaaata
      661 taaatacaac tttaaataat cttcctaatg ttgtagaaac ttttggagaa gtaggagaaa
      721 atatgaaaga tgtaacagaa gtagttactg aagttacagc agattttctt gttactaaag
      781 acagtgttaa atcaaatatg gatgttatag ttgatatatt gactataatt aaaaacattt
      841 ttctagataa aaaataataa gttaatataa aataagccta taagatataa tttaaacgac
      901 cttaaaataa catttataag gtcgtttttt attagcttga tttgacacta tgtgttttta
      961 ataattggta atattcctct ttatcatttg aacatatata tctatgcttg ttgtttcaat
     1021 tacatccata agaacatctt tacattcaag ctcaaaatct tccattcctt ctaaatattc
     1081 atcatcttca tcaaaaaaga tataagaatt atatagccat tcttcaaatt tttcttcaag
     1141 tatatcccaa tacgaactcc aaaataatcc acaacctgat atataagatg ctctactttg
     1201 atttatataa acctccttta gaagcttttg taatctactg ctataaagaa aatctgctct
     1261 tccactgctt gatattaatt catttaaatc ttctattgac attaattcaa ctttaccact
     1321 tttatcataa ggagtataat tttcatcaac ccatttatgt ggtttgtttt ctctgcatac
     1381 tgttgctatc aactctacaa cttttgttct tatcattttt ttatcctaac ttatttattt
     1441 tttataaaat aatcatgatt ttataattta tctatttctt gtaatgctaa tttaacagtt
     1501 tcatatctat cagcaccatt tattactaca tcacctttta caagtttatt agcttctcca
     1561 cctacaacat aaaccttttt acctaaacct tgcttataac tagacgcctc aattattaaa
     1621 gaatctttca attgccaatg taaaatctga gcagccactt tatcagcatc acctgtataa
     1681 acgattacat tttcatacac tttattttcc cctccccctt gagaatttga tatagatttg
     1741 tttagtatac cttctgctat caatttagca actttatctt tattattgac ataataatct
     1801 gtatctgctt tactatctac aaaacatact tctattaata ttgctggtgc aatagtatga
     1861 ttaagccaat aaagacctct aacatcagac tttgcacccc tatctttgaa tactgtagct
     1921 aacctagtat tcactctttc tgcataggtt ttgccattat tagtcttata tattgtttct
     1981 gtccctactg gagttgaagt tgtagcattt gaattaaaat gaatctgaac agccaaatca
     2041 gtttcctgtt tatttgctat agcacattgg tctgctaaat gattacttga ttcatcaact
     2101 cttcctgtat aaacagtagc tccacctatt ttaagccatt taacaattaa gtcattcagt
     2161 attcttgttt ctttgctttc gtttatatac ccaacagctc ctgtaccttt tccaattaaa
     2221 gtgtgccctg gtattattac tactttcaaa ctaacatctc ctttacaatt aaatttaact
     2281 tctatttaat atatgcataa cctagaattt tgtttctttt tatatcttat acttttatta
     2341 taacccattt tgtaaaaata tagaatatta agtaaataat gttaaaatta taattttgta
     2401 tataagacat tacttgattg caataatttt atagcatatt catctattct atatccagtt
     2461 ctataatata tatgtttaat tccagcttga attatagcct tagtacaatt taagcaagga
     2521 aagtgagtta cataaataga gcaatcttta actgaaatac cttcttttgc acaataataa
     2581 agggcatttt gttcagcgtg aatggttcta atacaatgtc catctctcat ttcatgacca
     2641 acttcactac aatgcttatc tccagaaata ctaccattat atccagtaga cactattctg
     2701 ttttctgaat tgacaattat agctcctaca taagctctat cacaagttcc tctttcagct
     2761 actgtttcgc acagtctcat aaaatattct tgccatgagc atctattttc aagtttttta
     2821 ttcaaatcat atacctccaa aatttatatc taatatataa gatttatacc ttaaaaataa
     2881 tattaaaaac aaatttatac gataaataaa atttattaat actgttttta gtctaaatta
     2941 tttcatttta aattaaagtt acaaggaatc taagattata tatatatttc ttcgtaatat
     3001 gaatgttaca atataaaaaa ctgtctcaat aaaatagaga cagttttaca gtcaaagttt
     3061 ataatacaag ttcatatttt tctttaaatt cttttacagc ttcgggccat gccttttgtt
     3121 ctgtaatacc tttttcttta gctattttaa ttgcaatctc tcttatcatt gcagcgcctt
     3181 ctaatattcc catttttaat ctcccttcaa atatcttttt gtatagaata taaatagtgt
     3241 taagatttat tacatgctgc atatacaata tctatacaac acataacttg taaattcctt
     3301 ttattttcat acgtctttaa ccgacatata attatattca ataagtttta aaattatgaa
     3361 ctcaattata attattttta tttttttaga atctaaacac taaaaattgt aggtaaattt
     3421 gtcttatgcc tttataaata tttctacagt tttaattact ggacatatct atttttatta
     3481 taaaaagcga ttaaaaatgg agaatatcct cataaaaact atatataagc aaaacggtta
     3541 aaggaaaaag aagaaattta attgaatgtt aaaatgcgtt gcttgaagca atgggcagtt
     3601 ttgcctataa tatcggtaac aactgaaaga aaggtggtta gccctaatgt taagcgaaaa
     3661 cattaaaaca atcaggaagt caaaaggact ttcacaacaa gaacttgcta taaaactgaa
     3721 tgtagtgcga caaacaatat ctaaatggga acaaggagtt tcccaaacaa aagaatatta
     3781 aagaataacc tgtgtttacc tgcttacgaa tttataaaag taatattttt tagtttcaga
     3841 taaaagttta atacttgttg atacattgtt aatatgataa aatttaacgt taggaggcga
     3901 taaaatgagt aatattatta atgcaatata taatctgatt aataaccctg ttattgaatt
     3961 aagagagttt tacaaaagta aaaacagagc aaataatatg ggtgatgctt tagaagagta
     4021 tgttaaagat ttatttgcga atacaatcaa tgaacaaaat gaacagagca gattgaaaaa
     4081 aatttcggat acattctcgt atttaggaaa tcaaaataat cctcctgata gtatattaca
     4141 tttgggagat gctattgaag taaaaaaaat agagggtaaa aatgcaagtc ttgcattaaa
     4201 tagttcatat cctaaacata aactttatgc taattccaaa ttaataacag aagcttgtag
     4261 aaagtgtgag acatggtctg aaaaagatat aatttatgct gttggagttg taaatggttc
     4321 tatgttaaat agtttatgta tggtatatgg aatagattat gcagcggata actctactta
     4381 cgaaaaaatt aaagaaataa ttgttagtgg ggtaaataat attccagatg ttgaatttgc
     4441 tcctactaat gagatagcaa aagtgaaaaa agttgaccca ctagggataa ctgatttaag
     4501 agttagaggt atgtggtcta ttgaaaatcc ttttaaggtt tttaactaca tatacaggag
     4561 agactttgat aaaaagttta attttatgtg tgtaataaga gaaactaaat attctagttt
     4621 tgaaaacaaa tcactaatag aaaactgtgt gggaagtatt caaggattta acatcgttga
     4681 tgttaaaatt aaaaatccca ataatccagc tcttttagaa aatgctaaac taattacttt
     4741 tcaaatatag aatgtaagga gagaataata aaatgaaatt agttagtctt ttttcaggag
     4801 ctggaggatt agatttaggg tttgaaaaag ctggatttga gataattatg gctaacgaaa
     4861 ttgataaaac aatttgggat acatatgaaa aaaaccacac agctaaatta ttgaaaggcg
     4921 atatatctaa gattgcctct agggaatttc ccgaatgtga tggaataatt ggtggtccac
     4981 cttgtcaaag ttggtcagaa gcaggaagtt tgagaggaat taatgaccca agaggtcaat
     5041 tattctatga gtatattcgt attttaaagg ataaacaacc aaagtttttt ttagcagaaa
     5101 atgtgaaagg aatgatggca aaaagacata acgaagctgt aacaaatatt attcagcaat
     5161 ttgaagaagc tgggtataat gtttttatta aattattaaa tgcagtagat tatggagtag
     5221 ctcaagacag aaagagagta ttctatatag gttttagaaa agatttaaat attaattttg
     5281 aatttccacc tgcttttaat gagaaaaagt gttttaaaga tataatattt gatttaaaag
     5341 acaatgctct tcccgcctta gaaaaaaata aaactaatgg tatgagttgt aaagtattga
     5401 accatgaata ttttactggt gcatactcaa ctatatttat gagcagaaat agagtgagaa
     5461 gttgggaaga gccagctttt acagttcaag ctagtggaag acaatgtcag cttcatccac
     5521 aagcacctaa aatgattaaa tttggaaaaa atgattgtag atttgttgaa ggtcaagaaa
     5581 atttatatag aagattgagt gttagagaag cggcgagagt tcaagggttt ccagatgact
     5641 ttgagtttgt ttatacttct ttaaatgatg gatataaaat gattggaaat gcagtaccag
     5701 tcaatttagc ttatattttg gccaagcaaa taaaaaaata tttagagaat aattaatgca
     5761 ttatgatata ccgacgaagt gatccagaca cggtggtggt cgtgagacat atcgccacgc
     5821 cagcttgctg gcgagtgtct ggttcacttt ggcggttggg cgagcctgcg agctttctaa
     5881 gcccccgtta tctaacaagg ggggcataaa aaacaagaaa caaaatacat tagcaagcgg
     5941 aaaacactgt atgtacaagg agaaaccgca ttttgtacag gtgtgaactc agtagggtgc
     6001 agttaacatc tgcatttgaa aattgaatat ggatattaca aggcagttga tttaataatc
     6061 ggctgtcttt ttttatgcaa tgaagaaagg aacgtgattt tatgaataaa aatacttttg
     6121 cagaagaatt aaagacaaaa cgtgaggaat acggcattac acagacaaga ctcgccgttg
     6181 cctgaggtat cagccgttaa tactacaatc gcattgagaa cggaaaaaca atcataagtg
     6241 aagaactgaa agcagaaatc tataagcagt tggaacgatt aaatccatta gagactatgt
     6301 tcatactggt tgactatttc cgtgtctgct tccctaccac agacccgttg accttttgca
     6361 tatcaagccc agacatatgt tccatgaaga atacagacgc tactgctatg aggaacagta
     6421 tattctaggc gatattaccg tgcttgtatc ttccaacccg tcattgggtg ttctattgga
     6481 gctgaaaggc aaagggtgca gacagataga gggctatctt ttagcacagc aaagaagctg
     6541 gtttgacctg atgatggact gtctgacttc tgacagcgtg ataaaacgtc ttgaccttgc
     6601 cataaatgac agggtcggtg tgctggacat tccaaagctc atagaaaaat atcagcatgg
     6661 ggaatgtatc tcctacttcc gcagacagaa gcgatatgac ggaatagaca agaacggcaa
     6721 tgatatgccc gaaagcacag gaaatactat gtatatcggc tcaacacaaa gtgagctgta
     6781 cttctgtatt tatgaaaaag cacgtcaaga atggcattgt cattgaggac agcgttttct
     6841 atcatcaatc actatgtccg ttttgttgat ccagaggaag acaagccaaa gaaccgctgg
     6901 aaactcaatc tagaatggga ataattcata ggagataacc gaaaaccgat acggctgaca
     6961 acacagccag agccgttcac attggaaaga gcattgcatt ggctacaaaa acaggcagca
     7021 cctacgctca aaatggtaca ggaactggac aagatgaacc acacgaccat actggacgat
     7081 atgctggagg atagcgagct gaaagacaag catagacatc tgctggaact ggaaaaaaca
     7141 aatattgaag ataggataga taccgccgtt tcaaatgaaa atgacggtat tttttagttt
     7201 ctttgaaaaa tctgcaacaa aacatcagat tgcgtacaga tatggtgcga gaaaagaaaa
     7261 ttcaactttt ttgaaaaatg gttattattt acggctttgc tgtacagata tgagatgaaa
     7321 gaagaaatat tttgaaaaac ggaagcaaaa tgacctatcc gttccctata tggagcgaag
     7381 aaaaaaaatc agttttctta aaaagaggtc attatttatg cctttgctgt ttctatatgg
     7441 tgtgaaagaa gaaaaataaa aaaatcttct gttttggtta gcaaaacgct ctttgtagga
     7501 gagttttgta ttcaaatgga catttgtagt cgagtcctgg tgtgaaaaga tgaaaatgtt
     7561 tcatttcagc ttaacaaatg acactttctg ttccctatat gatgtgaaag aaagtgaaag
     7621 gatttttcaa aaataaacca tttcgtttgt actgctaata ggaaaaagta aaaaaatcaa
     7681 aaaatcggtt acattctcca ctaccgctaa cgcggtatat gtgaaagaaa aatttcttct
     7741 tttatgttct ttgacaagtg aataaagcaa ttcaatacgt ttggcagaca gcgagccgtg
     7801 gaaacagata cgccatgacc tgtcctgtca gataatgata ctcccgtaca gccacagctt
     7861 gaacgctccc agcgtcgcat gcaatgggta cgactacatt ggataatgca gaggcggaag
     7921 tcctgtggac tgtgacaaaa gccgttgaat tgcttgtaaa aaaatatcat agaaagaggg
     7981 catgtgctat tgataatcca gtaaagacaa tgcagaagaa aagcaaacgt agcattggca
     8041 acagaggaaa aacaaagatt gtagtaaaag agcatttttc caaaaaagga aagacaatgg
     8101 aagaactctt gactgatgtc atgctggaaa aagcaaagca gacaatcgca taaaattggt
     8161 gcagttgtaa gaaatagatt gaatgagaaa aaagacccgt gttatacttt acttgcaagc
     8221 agatattgta aacacgggtt agttataaag gaggataact gacaatgaaa caacagattt
     8281 acaatactgc actttatctt aggttaagtc gagatgatat gaattacagg gcgaaagttc
     8341 cagtatcacg acacaaagaa acatgttgcg tctgtatgca aaaaaacatc attttacggt
     8401 gcttatgttc cactgggata taagaagcac ccagacatca aagggaaact actggttgat
     8461 gagtgaaaaa atggattgtt gaaaaaatct tttcattagc ctatcaagga tatggaagtg
     8521 caaaaatcac aaagcaactg cgagaagaaa aagtacccac atcatcatgg ctgaatttta
     8581 caagatacgg aacatttgca catatctttg agggaaagcc agaaagcaag cgatatgaat
     8641 gggctataac ccatgtcaaa gcaatattga agaatgaggt ttatattgga aacaacgtcc
     8701 ataatcgaca gtcaacggtt tctttcaaga gcaaaaagaa agtacgaaaa cccgaaagtt
     8761 aatggtttcg agtagaaaat actcatgagc cgattattga caaggaagtg ttctatcgtg
     8821 tgcaggagca gataaaatcc agacgcagac agacaaagaa aaaggaaaca ccgatatttg
     8881 cagggcttgt caagtgtgcg aattgtggct agtctatgcg gtttgcgaca aatacggcaa
     8941 ataaaatgcc atacagttat tatgcttgca gtttctacgg acagtttgga aaaggctact
     9001 gttctatgca ctatatccgc tatgacgtgc tgtattaatc cgtagaaaga ttgcaatact
     9061 gggctagagc cattcagaag gacgaagaaa aggtgctaaa caagatacag aaagtcggca
     9121 acgcagagag aatacagaaa aagaagaaaa aagcaagttc attgaagaaa gccaagaacc
     9181 acaaaacgag attgaccgct tgtttgcgaa aatgtatgaa gatagagcct acgagaagat
     9241 aacggaacga aactttgtca tgctatccag caaatatcag caggagcaga tagagctgga
     9301 acagcagata acgagcctaa gagaagaatt aagtaaaatg gaacaagata tgataggtgt
     9361 tgaaaagtgg attgagctaa tcaaggaata ttccgtacca aaggaactga cagcaccgtt
     9421 attaaatgca acgatagaaa aaatccttgt tcatgaagca acaacgaata aggataacga
     9481 acgaatacag gaaatagaga tatactatcg atttgttgga aaagtggact gataaagagg
     9541 ttcatatctt taactaaggg aaactggact gtcggttccc gattctgata tgttgatttc
     9601 catatcagag gtgcttgaaa cgtccgtaag cactttactt ggagaaacta ttgttgagtc
     9661 ggaagttgat aacttaaaag tgatttctga aaaattagag attataaact tgcaacttgc
     9721 acaaagaaaa aacatgagaa gaaaaatgtt tcattggtta tttattttat tatgtgcagt
     9781 catattaata gtttttgtgg tcttaggggg actaaatagt ccttacttgg attggaatta
     9841 taccgaccct gaaaccgctg tttttggagt agcttttcat gcttttgagt ggctatttgt
     9901 cagattagta ccgattgtcc ttataggagc aattattgga atctttgtga cacgtaagaa
     9961 aatatgaagt aactctgctt tttataatac agcaagcgtt ccttaaaagt gtcattctac
    10021 atggcaatcg aggacaacta tgtaaggaag aacctttttg atttccaact aagcgaagtt
    10081 attgaggacg acagtaggcc caaaaccgca ttaagtaaag tacaggaagc aagactgctg
    10141 gacttatact ctcatgcgtc agatgcagga aatgcgaggt ctggtagcat gatttacaac
    10201 gatttgtaca acgactacga gtagaaatat atggagataa gtattatcta cgaaatcaga
    10261 aaagaaatga cttgataatt tacccatgtt ataattattg atgtgaaatt aataaatcga
    10321 aattcctttt ttagatttaa caatactgag taaggaaaaa gtgaatgact tttgcaagga
    10381 ggtgttaata tcaggcaagt aaagtctttt gtttttccag ttatatcctt aatattctta
    10441 gaccaaatta gcaaagttct tataggatta ttcttaatgg actttgaaat tgatataatt
    10501 gggaaatttt taagattcaa tcctgttcaa aatacaaatc tctcttatgg gggaaacttt
    10561 attggtattc tatctaattt atgggtattg gtattgttta acattttagt tatattagtt
    10621 attatatctg gatatgcttt ttataaatca aaaaatgaac aaacaagcta ttcagtaaaa
    10681 gtaattatgt cttgtggact tgctggtaca atatgtagct tgatagataa attattttgg
    10741 ggaggaagtt tagatttttt gcagatacca agctttttta ttttcgactt aaaagactgc
    10801 taccttactg ttgcagaaat aatatttgtt gtcataggaa ttttgcataa tagagaaata
    10861 tcaatgaaag aatacatata tttttgctat cgtcagttta aaagataaat ttagaagata
    10921 aatttaagtt tcaaggtaaa cttatttaat cacagcaaat agttggctta cttaagcttt
    10981 tgggctaaac tgtgagaact aattaagtct ctgctatcta acaaacggag cacaaaaata
    11041 aatattaaat attaaatata aagcacagtt aattttgatt aaaaagttga ctgtcttttc
    11101 ttttgtagaa atttaagcat aggtaataaa gaaatgactg catttaagca ttatgtatat
    11161 aattggaggc aataaaaagc cagcaaagcc cataaaatag agctgtgcta gcaacatgat
    11221 gagttataag aagataatca aaaacttacg ctttatttta atttaaaaaa atgattactg
    11281 taaagaaatc attttttatt ataatcactg atacatcaaa tattctaaat aaaattttta
    11341 tttatctgga attgtatatt ttttctataa tattaactac agcgtacatt gcccaagcac
    11401 agatagccaa tacaaatact cccatcatta ctaaatctaa tttaaatact tgagaaccat
    11461 atactatcaa gtagcctatt ccatatcttg aaactaaaaa ttctccaact attacaccta
    11521 cccatgccat accaatatta attttagtta aatttattaa atttcctgtg tttgcaggta
    11581 atatgagttt aaataatatt tgagatttag tagctccaaa gctttttaac atcttaattt
    11641 tttcttcatc tatattcata aaataattat aagctgaaag tatagtaaca acaacagaaa
    11701 tcgttacagc tgttacgacg attccttcta ttccagcacc tacccaaact ataattatag
    11761 gagctaaagc agtcttagga agagcattta aaactaccaa aaatgggtct aatatttttg
    11821 ataatttttc tgaccaccaa agagcaattg ctaccaaaat acctaataca gtacctataa
    11881 caagaccaag aacagtttca tatacagata ttccaatatg tttaaataat tgaccactaa
    11941 atgcatattg aataaataaa ttgtaaatat cacttggttt gctaaataga aatgtattta
    12001 taacatttaa gtcagctaaa agttcccaga ttacaataaa accaattaaa attaatagct
    12061 ggtagaataa tacatttctt ttttctcttt ttacaccttt aacatagttt atatatcctt
    12121 cagatctttt attaagcctc atgtgtatct agctccttcc ataatatgtc gaaatacttt
    12181 tgaaaatcag gaactttacg tgttttgaga ggcgtttttg ttccatccat gttaagttca
    12241 atatcgtaaa tattttttat ggtagctggt cttttagata gtattgcaac cttatctgat
    12301 atgcttattg cttcaccaat atcatgtgta accaaaatag tagacttatt ctcattctta
    12361 ataatactat aaacatcatc acaaactaat aatctagtct gataatcaag agctgaaaaa
    12421 ggttcatcta aaagtagaat atctggattt acagaaagtg ttctaataag cgcaactctt
    12481 tgtctcatac ctccagataa ttcttttgga tacatacttc taaaatccca taatccataa
    12541 gtctttaaaa ggctttcaac tctatctaaa gattttttat cttttttgcc ttgaatttcc
    12601 aatccaatag ttatattatc cattatattt ctccattcaa gcaaattatc tttttgaaac
    12661 atatatccaa tatttccagt aatctttata tcaccactag tcggttttaa aagattagta
    12721 agaatattta atattgtaga tttaccactt ccagaaggac caagcaaagt taatatttct
    12781 ccttctttca agttaaaatt gacatccttt aatacttcaa gctcgccttc cttagtgtag
    12841 aagttcatat ttatattttt aacttctact atgttgtcat tcattatgcc accccctata
    12901 acttttagct cgtatatatt atgttattaa caatatgacc tatttgtacc atttttcata
    12961 aatataatat aaatattttg tttatttacg tattttctta gtaacttgta cgtataaatt
    13021 atagataaat aaattataag gtaggggata aatttgagta acataataac gaatttaata
    13081 gagtcaatat ttactaatcc aataactata ttgtttttac ttgcaataac tgtgtattct
    13141 gtttttaaaa taatagaaaa taacagagta tattcatttg taagttcaaa attagacgaa
    13201 atcaatagag attataagac aagtgagttt tataaaaagg taagggatga ctactataca
    13261 tatagtaaag aaaatcctta tgcagacgta aatataactt catttataga ggaagttgta
    13321 tctgatttaa agcataataa tttgccattg ttagaaaaaa ttagaagtat aaaaaattca
    13381 tcttcaataa gtatattact tggggtttta ggtacgtttg ttggtctttc tacaatgctt
    13441 ttatgtgttg atactaaaga cattataaat tcacttcctt caactattag tagtatgcaa
    13501 actgcattta caactagtat atttggagtt gtattttctc taattatagg ttactttact
    13561 aagataaaag attgtgaaca tgttttgata caaataatgt taaaatctga aaatttacta
    13621 acatctgaaa taactcattt taagtcagaa agaatggatt taaaagtaga agaggtaaaa
    13681 aatacaataa aacaaataag taagtctata gaagctattg aaaaatttga taaaatatct
    13741 aaagatttaa atgactttaa tgatgaattt atcagtggta tagaagcttt aaaaagcctt
    13801 ttagaaggtt ctcaaagttc tattaagaca tttgaccaga gcgtaagaaa attagataaa
    13861 caatttaata ttttaaatat aaaatttgta aagctttttg ataaatatga taatcaagat
    13921 aatataaata aagagatttt atttgatata aaagaatctt caaaaaatat atacaatgcc
    13981 actgaaagcc aattcaaaat tagagattat ataaaaaata taaatgcagg atttgctcta
    14041 tatgaaagaa gtgctcaaga tttattaact aaattaatga cccatgaaaa taaaatctct
    14101 caaaatcaaa aaatcctttt agatgaaaag tttaccttag atgactcaat aaaaaatctc
    14161 tcaagtatta ttgaaaactt ttctaatgac ctacaagtta aactggatat gatgtttgaa
    14221 aattctttag atattcaaga taagttagat gttatgttta ataactcttt tatgaatgat
    14281 gaagttcctc ttgatagtga agaattattt aaggatgatg ttaataatgt atttaatcct
    14341 tttagtgaag aaatttatga gatagaggat aaagaaataa aggtcatagg tgaggatgag
    14401 ttaaatgaat aataaatata gaagaactgt taacagagaa tttgaaaaga gtagcttttg
    14461 gccaacattt acagatttat tatctactgt attgatggtt gtgattctta tcttatttag
    14521 ctcagaaagt atttcaggtt ctgttgaaca agatttagct aagaatgtca atgcttctgt
    14581 tgaagaaact tttaaaaaaa gtggaatacc tgtgaaggta gataaatcta atgggcaagt
    14641 aacttttggt gaaaaaacca tgtttgatgt agatagtgat gttttaaaac cagaggctaa
    14701 agaaatgcta aaaatgtttg ttccaaagta catagagact atttataaag attatggtga
    14761 ctatatatcc aaaatagtta tagaaggtca tacagatgat gtgggaagtt atatatacaa
    14821 cttagattta tcgcaaagaa gagcatatag tgttgctaaa tttatagtgg gagatgaaat
    14881 aggtgactat aaatacaaag ataaagtgac taagcatatt attgcaatag gaaggtcaaa
    14941 agctgaactc atcaaaaatg gtgacaatag cgtgaataga gatgcatcta gaagagtaga
    15001 actaaaatac gaaataaata ttaatcaaaa taaataatta aatttttata aaaaaaaaga
    15061 aggcagttgg tgtgagcttt aagccttctt taaggaaaaa gtttatttat ttttgttgat
    15121 acttaacata ttatagtttt tagaagcagt ttgcaatata ttaaacgtaa atggtcgttt
    15181 aaaatgcata actggatact actgatgtaa aatatttcat tttatataat ataaaaatta
    15241 tacagtgtca attttctata ttattgtact ataatctatt atttaaaata ttatgtaaaa
    15301 aaatatcata aaaacgcata tcatgttata attactagag tattatgaat atggaggcgt
    15361 agtttgtgat agaaataagt ataaaaaata aaaaaaattc taaaattaaa actttgatac
    15421 ttttattgat agtatctata tcgatttatt taactggttg tactacatca gataatggaa
    15481 ataatcaatc aaataataat gattctttgt ctagtagtat atcaagtgat aagaatgtaa
    15541 aaattcattt tattgacact ggaaatagtg atgcaatatt gattcaagat ggtaaaactt
    15601 ttatattgat agatggggga gataatgatg atgagggatt aatggtagat tacctaaaca
    15661 atcaaggtgt aaaagatatt aaatatctta tagctaccca ttctcatgca gaccatttag
    15721 gtggacttga ttctgttgtc aaaaacttta atatagaaaa tgtttttgta tcgaatggtt
    15781 cagcagaaac aaagagttat agggacttta taaatgcttt agcaaataag ggtttatccc
    15841 caagtgtacc tcttgaaaat aataagttct atttagaaga ttcttatttt gaagttctaa
    15901 atacaaatgg gggagatact actaatgaac aatctctagt ccttgtatat actaatggga
    15961 atgataaagt tttatttaca ggtgatgctg aagaaggtac cgaaaaagaa atactaccta
    16021 agcttgagaa agtagacctt ttaaaagtag ctcatcatgg ttctcgtagt agttcatctc
    16081 aagagttttt agataaagta aatcctgaat atgcggttct tttagtaggc aagggtaatt
    16141 catatggtca tccacatcag gagacaatga ataaattaaa aaaaatggga gtaaaagtac
    16201 atagaagtga tgagtgttca gatataatat ttgaatcaac aggtgatgga gtatttacaa
    16261 gttgtaaaga tggaagttat aataaaggcg ttagagaaga tggaaactct ggaaaatcaa
    16321 acagtaatac aagtaaaaat gattctttta aaaataaaca aaatacatcc aattcagaag
    16381 aaatagtgta ttttactcca aaaggtaaga gttatcactc tacaaaaaat tgtagtgggc
    16441 tttcaagaag taaaaaaata ttatcaggca caattgcaga aagtaagaaa aatgacccat
    16501 gtgatatatg ttatggaaaa taacaaaaaa acaaccaatt tatctataaa gcttctgaac
    16561 tttaaatcat ttaaaaaata aaataaatgc ataaaaaaat aagctataat caatagataa
    16621 cacattttat ctatacgaat agcttatttt ttcaagtatt ttttaatagc tatttaataa
    16681 ctctaaatac accagttaca atacctacaa tttcaacttc tgagtcattg agcataattg
    16741 gttccataaa atcattttct ggctgaagtc ttattaaatt tccttcttta aaaaacctct
    16801 taactgtagc agattcacca tttataagtg caactactat ctgagaattt aaggctgtat
    16861 tttttttgtc tactacaacg taatcttcat ctaaaattcc tgcattaatc atactttcgc
    16921 cttttactct taatacaaaa ttgttactgc cctttactaa actggcaggg aatggaatgt
    16981 attcctctat gttttgttga gctaaaatgg gttctccagc tgttatttga cctactaaag
    17041 gaagctctat tatttcttga ttagctccag aaacatcatt tactttacta cgttctaaaa
    17101 cttcaatagc tcttggttta gtagggtcct ttcttatgta tcccagtttt tcaagtttat
    17161 ttagatgaga gtgcacagtt gaagtagatc ttaaccctac agcagtacat atttctctta
    17221 cagcaggtgg ataacctttt aatatgattt gagactttat aaattccaat atcaagactt
    17281 gcttttcagt tagatctaaa tacataacat cactcatttc caaacatatt ttgtttttat
    17341 ttccatacac tttatatttt taattataac ataatccatt tatatctgca aacatttgtt
    17401 cctgattttt aatcgtcctc aatccattca aaattcttga ctatatcacg ttttacattt
    17461 ttcttatgag ctgcataaag ctgagtagtt gtgacattat catggtcaag taagttttga
    17521 acatcatata tagaaaatcc tgtttggatt agagaagttg cagccgttgc tcttaattta
    17581 tggggactat atccattgtc tctagtggta tcaagtggaa tagatgtata tttttttacc
    17641 agtgttctta tagcctttgc tgtaattctc ttattttgaa gagacaaaaa taaagcatct
    17701 ttttcttcat catttaatag ttcatctctt gttctttcgt tttgcaaata atcttttatt
    17761 acatgttcac aagtatgatt tataggcatt aaaacttctt tcccacgttt tctatatatt
    17821 ttaaattcac ctcttgaaaa attaaaagag gagatattga gttctctaag ctcatttaat
    17881 ctaagcccgt atgtaacaaa taatgctagt atagctttgt cacgtaattt agtttttctc
    17941 caatatacct tttctttctc tgtaagccct tcacctgttt ctactgattc aagcatctta
    18001 gcaacttcat ctatctcaag gcgcttaata gcatctggct gaggtttagg aagttttatt
    18061 ggattaaatc catcagttat attattatct atctgagagt ttctgtataa gaatttaaat
    18121 agagtagaga tggatgattt ttttcttgct aaagctctat tgttattttc aaaaatcaat
    18181 gtgttttttt ctgtattttt gtaatatcta ctgcaataat cacctaagaa taaatttata
    18241 tctcttgatt ttattgtatt aaaatcatct tctgtaatat cttttacaca atctgcattt
    18301 gttaattctt tggtttcaat caaatatgaa caaaaaaaat taatatcttc taaataagca
    18361 agtcgtgtag aaactgcaac agaacctttt aaatatataa aataatcctt cataaataca
    18421 ggaagtttat tttccaaaag tagacatttt tctatgagtt cattttcttt gataacaata
    18481 ttatcaggtt tatctgattt attatgtact tttataattt tcttatcatt attcaaaatt
    18541 aaacctccta aaactaaaat ataaatcttt tatattattt tttgatattt atactaagtg
    18601 agtataaata tttaatttaa tcatttttta catttttaag agattagcag tgctttagat
    18661 gaaattatca ctaactattc cctaaatcca catttaaaga ctagttgaat ttaagtactc
    18721 ttttttattt ctcaatatat ttatagaaat attttaaaat atattgagaa attttttatg
    18781 ctctactaaa atctttttta tataatattt ttatatgttt ctaacgaaac caaatattag
    18841 taaaatatgt atttatttaa ctaaagtttt taaatgggct gactaagctt aaaatatagg
    18901 atattatgag agttactatt gaaaaaataa accctattgc cataaagaaa cttataaata
    18961 tgttatactt aactattaaa ctggatatat tcaagttatc aaatccaaat aagtatgtaa
    19021 ttgaacttat tacaaaaata actataacta taaagctcag accatttaaa ctatttttta
    19081 aatcagcact actaagtgac atatgtgtag atatagaata cattaaaaac ataaatataa
    19141 aatacttcaa tgaatataca ctaggtgtta acaacattat tcttataaaa ttggaaatat
    19201 catctaaaat aatgtgtata aaaccattta tatttaaatt attaatcata cttacatata
    19261 actttaaatc tatattttga gtcactttca tataactatc tggcagtaat atcttaaaaa
    19321 ttaaaataat cattattgtt ccacctataa taggtgcaat tcctataaag aaatttccta
    19381 tactttgata aagattatct tttttatagc tatgacttac atatcctagt ataccatcat
    19441 ttttactttc tattggtctg aaaaatttaa ctgaatttat tttatgcatg aaaatcaaac
    19501 acattatcaa atgactcaat tcatgaacaa acgttcctat agctccagtt acaataattc
    19561 cagtttttcc aaaagatgaa aagataaatt tattatttag attttctgta atgctaaaaa
    19621 tcaatccaaa tacaataaat attcctatga aacaaaaaat atttattaca cttattttta
    19681 aaatttcgat tatataaatc ataagttaag tcctcatgta aaagtattta actactctac
    19741 ttcatatcca ttacagctta aaattttaca agcattttct atatcttttt cttttactaa
    19801 gatatagtct gtatcatacg tagaaattgc aaatatgctt attttgttgt ctgctaacaa
    19861 actacttaac ttagataaaa ttcctacaag tgaaaagtct aaaggaccta gtatcttaag
    19921 tattctccac tccttttctg acttgacatc agaaggaata ttattattta aacatacaac
    19981 acttaattca tcatcagttc tagttataga gtaaaacttc tctgtatcta tccatgttgg
    20041 tattttactg tcattattta atctacatac tgcatactct tctaccaata attttaattt
    20101 cataatgtat atttcctcca atcttatttg tttacttaag attatacatt aaaagttaca
    20161 taaaataaaa catataaagt aaaaaaggtt cactatagtg tcctatttct atagccaacc
    20221 tttaaaactt aattaaattc taataaagag atatattatc taggttcaat tataagttta
    20281 atggctgttt tcttatcacc ttcgatttct atatcagtaa atgcgggaat gcaaactaaa
    20341 tccattccac taggtgcaac aaaacctctt gctattgcaa ttgattttat cgcttgattt
    20401 agagctccag ctcctatagc ttgtatttct gcaacgcctc gttctcttag tactccagcc
    20461 aaggccccag ctacagaatt aggattagat tttgatgaaa cttttagtac ctccatttca
    20521 acatctcctt gttaatttgt taaagtatcc attacctaaa gtactaaatc ataattgcca
    20581 tttactaatt acaatcatca tttaaagtat acttttataa aaatatactt ctataatcgt
    20641 tttaagaaaa cctttatttt ttttaaccat tttattatga aaaaactaca aatttcttta
    20701 tattctataa gcattttcca atatttctaa attttttata tctttgagct ataatttcct
    20761 ctttatccat atctttgtaa tgatttaatc tttctaaaat ataagctttt atttcttttg
    20821 aaacagcttc tacatctttt tgtgctcctc caagaggttc ttttataatc ttatctatta
    20881 tttttaaatc atataaatca ttacttgtaa gcttcataac atcacaagct tctttagctt
    20941 tagatgaatc tttaaataga atactagaaa gcccttctgg agaaatcaca gaataaactg
    21001 aatgttctaa catacataca tcatttccta ctccgatacc taaagctcct ccactaccac
    21061 cttcaccaat tacaaaagtg attactggaa cagaaagttt actcatttca agtaagtttc
    21121 ttgcaatagc ttctccttgt cctctttctt ctgcttctag accacaaaaa gctcctgatg
    21181 tatcaataaa cgttattata ggtctattaa atttttcagc ttgtttcata agtcttaatg
    21241 cttttctgta tccttctgga tgaggcattc caaaatttct ttttatattt tcttttgtat
    21301 ctcttccttt ttggtgtcca actatagtaa cattaaactt tcctatctga ccaattcctc
    21361 caaccataga agggtcatct ttaaaaagcc tatctccatg aagttctaaa aaattagaac
    21421 aaatatgttc tatgtattcc aatgtcgtag gtctctttat atctctactt agtgttacct
    21481 tttcatatgc tgatagatgt gaaaaagcat cttccttaag cttttccaat ttctcattaa
    21541 gtgaatttat tttatcactt aaatctatat tatttgcctt agaaatagtt attaattgtt
    21601 taatatcatt ttctaagact tttatttttt cactattatt ttcaatcaaa acagttcctc
    21661 ctttatgccc tatttcttgt gcatagcaag aatttgatat aatacttcct tcatttttct
    21721 tctatctact atcatgtcaa taaaaccttt ttcaagtaaa aactcagaag tttgaaatcc
    21781 ttcaggaagt ttttgattta ttgtttgctc tattactctt ggacctgcaa aacctatcaa
    21841 cgtatttggc tcagaaatta taatatcacc aagcatagca aaactagcag ttactcctcc
    21901 agtagtaggg tcagttagaa ctgatatata tggcaatgat ttttcttcaa gcttagaaag
    21961 tgcttgagaa gtctttgcca tctgcataag agataccata ccttcttgca ttctagctcc
    22021 tcctgaagca gaacaaataa ttataggtaa attattttca gcagcatact ctattgaata
    22081 agttattttt tccccaacaa tagaacccat acttcccatc ataaaatcag gattcattac
    22141 acaaataact gttttaattc catttattct tccataacct gtgacaacag cttctgactc
    22201 ttttgttttt tccttataag agtctacttt ttctttgtat ttaggaaaat ttagtggatt
    22261 ttgaaattct acatcagaat taaactccat aaaagttcct ttatcaatca ataaatttat
    22321 tctttttctt gctcttaagc cataatgtct tgaacatttt ggacatacaa atgaattttc
    22381 ttctatgtcc tttctaaata catgactatc acaacctttg caataggtcc aaaatttatc
    22441 atctacacta ttctttttaa agttgtcatc taaagaaatc gtaacatact tagattcttt
    22501 tgatgataaa aattttttta tcataatcta tttactcctt ataatgtagc aaattctttc
    22561 ttcataaaga catcttctat aaatttggta tgatgagtat cttctctaaa ctcctcactt
    22621 gttacaagtg ccttttgaaa ttctacattt gtattaatac cttctacaat aatttcgtct
    22681 agagctctat atattctact tatacattct tctctagttt cagcccaaca tattaactta
    22741 cctatcatag aatcatataa aggtggtata gtatatcctg catatacaaa agtatcaaat
    22801 ctaacaccaa aacctccagg taaatttaat gattctatct tacctggtga tggagcaaaa
    22861 ttctttgatg aatcttctgc atttattcta cattcaatag catgacctct aaaagtaata
    22921 tcttcttgtt taaaaggaat tttatcccca ttagctatag atatttgaag ttttataagg
    22981 tcaagatttg ttaccatctc tgtaactgga tgttcaactt gtattcttgt attcatctct
    23041 ataaaataaa agtttttatc tttgtcaact ataaactcga tagtacctgc accgatataa
    23101 cctactcctt ttactatatc tactgcaatt ttatgaagtt tttgtctttc ttcatctgaa
    23161 aggtatggac ttaaagattc ttctattact ttttgatttc gcctttgcat agaacaatct
    23221 ctatcaccta aatgtattgc atttgaatgt ttatctccaa atacttgtac ttctatatgt
    23281 ctaggatttt ctataaactt ttccatatat aaatcactat tagaaaatgc tgctagagct
    23341 tctgaacttg ccatatcaaa atttgaaaaa agttcttctt cttttctaac gattctcata
    23401 ccttttccac cgcctccact tgaggctttt atcataacag gatatccaat ttctcttgca
    23461 acttctaaag cttcttcctt agttttcaaa acagattctg aaccaggaac aacagggata
    23521 ttcaattcca tcatagtttc tcttgctcta gatttatctc ccatcaaaat catttgttct
    23581 cttgttggac ctataaaaat aatattatta tcttcacatt gctttgcaaa ttcaggattc
    23641 tctgataaaa atccaaagcc tggatgaata gcatcacact ttaatttaag agcaagatag
    23701 attatatttt ctatattatt ataactctta cttatattat ttggtcctat acaaatagat
    23761 tcatcagcta tatatctatg aaaacaatct ttatctattt cagaatagat agcaacagtt
    23821 tttataccta gttctttaca tgttctgatg attctaactg ctatatcgcc tctatttgca
    23881 actagtattt tttttatcaa cttaaatacc tcctactaca atggttttat cttaaataaa
    23941 ggttgattat attctactag ttcttcatta ctaactaata cttcaattat ttcaccttta
    24001 atttcacttg ttatctcatt cataagcttc attgcttcta atatacataa agtgtcgcct
    24061 tcttcaacta catcgccaac tttaacaaag ctatctgcat ctggacttgg agaatcatag
    24121 aatgttccca tcaaaggagc tactactact gatagattct cattagatgt gacaggtgta
    24181 tcgtttacaa ctggcttttc taaagaaaga tccactactg attcttgttg tacacttaaa
    24241 acaggtgaac ttgtagattg agctttttta gatacttcta atcttaaatc gctactttca
    24301 agttttacat attctaagtt agttgagtct atggccttta ataattcctt aatttcattg
    24361 atgttcattt tgttaccccc ttgtattatt acctggtgct aatattatat cacaaaaaac
    24421 aaatttataa aagaaaaagt gctgactaga gaatcaacac atcaatatat cttttaattt
    24481 taaattaaca accagtaact tatatttgtt ttaattttcc attattaata taaattataa
    24541 gtataaaatg aataaaattc aatatttttt gcaaaataca agagaataca aattaatacc
    24601 tcgggaattt ttaagacaaa taccaatatt atgcaattta gacttcgctt tctttcaaat
    24661 tagtatcatt ttctttattt ttacatagga tgtactttaa tttagctatc aaaacattgc
    24721 tatataggtt aaaagttatt agaattgcag gaattataaa gtaaactcta gcacctataa
    24781 aaccaaaaac tactgtaata agtattaatc ttttatttaa agtaaaaaaa ggtatattaa
    24841 aaatactttt gtcggatgtt tctggagtat aagaatatat aactccaaaa atcaaagtaa
    24901 gcatacaaag tggaagtagt gtataacttg aaattattcc cagaaatcct atactttcaa
    24961 aatatttgct agcactattt gtataaacaa acatgtatcc atttaaatat gcgcatatac
    25021 atgatataat gccaaaagtt ccccctaagg attgagtgta ccaattaaaa aatttagatt
    25081 ccatatacct cacctcctaa aactgtatat ataatgatat gcatttaaat agtaaaatat
    25141 atctttatat gtttctgaat aaaattttgt taatttcatt aatttatgtt taagaaattt
    25201 gaagtatagt gtagtatata ttaatgtaga tgaagtttta tagtacttat ttcatagtgc
    25261 ttaatcaagt tatcttatta tggattcaaa agttgtttgt agggttttat attttgttga
    25321 gctgaaaaat ttattataaa tgaagggatg ttttcgcttt ggtagataca ttagaatttg
    25381 gtcttaaaat tttatttttt atattatcaa taatttggat gggtaaaata atgattttaa
    25441 gaactgataa gcaaatagta atcaatcctt tgcttattgg tatatctgca gtccttgtga
    25501 tgttgcatac ctcacagagt aacattgaat tttttggatt agatgtccaa tatataagaa
    25561 tagttttgta tataatatat tcactgataa ttttaatagg aatatgggct actaacagaa
    25621 gaaatggaat tttttagata atctatttct ataaaattta aaggttgcta gtaacaaagc
    25681 actaatatta gcgttctgtt actaacaacc tttattttga ttatacactt aaattgaaaa
    25741 tagaatgatt gaataagttg acagattatt ttacattgac agtaattaga acttcttgag
    25801 atgtatttaa atcatttttg tcgcttagta taacttttac cactgcttga cctgttttat
    25861 tacctttaat tatcatatct tcacatgaaa gtacactctt atcacttaca gatacaataa
    25921 aatttaaatc ttttggaagg ttatcagtaa cttcccagta tttataataa ctatcaattt
    25981 tcttcgtaat tatttttctc aaatcaatac tttcaccttt tgctatatca acattatgat
    26041 atttagagtc cagtttatac tgattatctt ttgtatcaat aaaatttaaa tcgcaattta
    26101 catccacacc attatgagaa agagaattta ctacatcgtc atcaatcaaa taattattgg
    26161 caagatttat tgaatccata tcttttagac ttaaagcttc cttaggaacc tcttttatat
    26221 tattattcca aaaaataaaa ttgtctacat tttcaagata tgatattcct tttaaatttg
    26281 aaatactctc accagttgca taaaattctc catctaaagc ctttatattg cttatagtca
    26341 tctcatctgg gtttatatat ctttttacaa ctgctcttaa atttgcatca ggaaaaatat
    26401 ctttaagttg attttcgctc aaaaccttat catttgaatt tattggactt aaagctgctg
    26461 cagaaataat aaatggtatt aaattagcta aattcatagt accctcccac ataaattttt
    26521 tattataata atttaatttc tatgagaggg ttctgtttcc ttgttatata tacctacttt
    26581 cgtacacttt attaatataa attaaaataa atttctacta ttaaacttat aaacatatta
    26641 ctagttacgt tttttagctt cacatctaaa tattcgcata ccttgtgaca taaatttgtc
    26701 ttcatactct gttgtaacat tattttcata ctcactattt cctaaatcaa gagaaatatt
    26761 ctttaatagc caattatttg cagcaatttc atttaaacta aactcgaata atttttcatt
    26821 atctgtttta aagtgaattt cgccatcatc ttccaaaacc ctattgtaca tttctaaaaa
    26881 tccagaatga gtgagtcttc ttttactcca tctattttta ggccatgggt cacagaaatt
    26941 tatataaatt ctcgataatt ctttagcttc aaagtaatcc aaaatattgc ttacatctcc
    27001 ccataaaaat agtatattat tcaaattaga agcatctgct ttttcaacag cctttaaaag
    27061 aacttcttcc ttaagttcca tggcaatata atttatgtca gggttctgct tagctaaagt
    27121 tgttataaac ttaccttttc cagtcccaaa ttcaacatgt attggattat catttctaaa
    27181 ctttaaattc cacttgcctt ttaatttgtc tatatcgtct cttaaaacat attttgtata
    27241 acttaaaagc ttttcatctg cacctttttt tcgtcttctt ctcacaataa ttctccttac
    27301 atataaaaaa ttagtaaaat acatttttat tatacattat ctcaccaaaa aataaaaaga
    27361 actctcttaa aatagaaatt tatttctata aagagagttc taaacttact aaaaacttaa
    27421 tcttagtggc accctgaaca accagagcat cctcctccac cttgatcttt catattttca
    27481 ggaataactc taaatccaaa tcctgtatct tctattatta tatctccata ctgactatat
    27541 tcatcagagc tcattacaaa ttgtaattct ccaacttcat aagttaagtc atcttctttt
    27601 ttctcatcta aagctaatcc aaaagaagga cctccacaac caacacctgc aaagtatact
    27661 cttatattat taggtttatc ttgattgtct tttaagatac tttttaaagt atcaatagct
    27721 gtttctggta aagtaatttt catcaaatca ttccctttca aatattttat taaaacaata
    27781 tctattatat acccatacaa ggtacataat caattagttt ttgcatattt tactaaaact
    27841 tctacctaat attacctaag gctttaaaat attttatata atattttatt accttaaata
    27901 aataccctat cataaac
//
LOCUS       DAEMHZ010000043        27703 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000043 DAEMHZ010000000
VERSION     DAEMHZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.043"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..618
                     /locus_tag="KPK72_001741"
     CDS             274..618
                     /locus_tag="KPK72_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG2488964.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            649..2250
                     /locus_tag="KPK72_001742"
     CDS             649..2250
                     /locus_tag="KPK72_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG2488965.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2269..3369
                     /gene="dprA"
                     /locus_tag="KPK72_001743"
     CDS             2269..3369
                     /gene="dprA"
                     /locus_tag="KPK72_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG2488966.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSAVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3402..5489
                     /gene="topA"
                     /locus_tag="KPK72_001744"
     CDS             3402..5489
                     /gene="topA"
                     /locus_tag="KPK72_001744"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG2488967.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5654..6439
                     /gene="codY"
                     /locus_tag="KPK72_001745"
     CDS             5654..6439
                     /gene="codY"
                     /locus_tag="KPK72_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG2488968.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6546..7811
                     /locus_tag="KPK72_001746"
     CDS             6546..7811
                     /locus_tag="KPK72_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG2488969.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7842..8612
                     /locus_tag="KPK72_001747"
     CDS             7842..8612
                     /locus_tag="KPK72_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2488970.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8721..9167
                     /locus_tag="KPK72_001748"
     CDS             8721..9167
                     /locus_tag="KPK72_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2488971.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9185..10378
                     /gene="nifS"
                     /locus_tag="KPK72_001749"
     CDS             9185..10378
                     /gene="nifS"
                     /locus_tag="KPK72_001749"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG2488972.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10432..10872
                     /gene="nifU"
                     /locus_tag="KPK72_001750"
     CDS             10432..10872
                     /gene="nifU"
                     /locus_tag="KPK72_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG2488973.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11161..12240
                     /gene="mnmA"
                     /locus_tag="KPK72_001751"
     CDS             11161..12240
                     /gene="mnmA"
                     /locus_tag="KPK72_001751"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG2488974.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12383..12621
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12689..15328
                     /gene="alaS"
                     /locus_tag="KPK72_001752"
     CDS             12689..15328
                     /gene="alaS"
                     /locus_tag="KPK72_001752"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG2488975.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15353..15610
                     /locus_tag="KPK72_001753"
     CDS             15353..15610
                     /locus_tag="KPK72_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG2488976.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15620..17791
                     /locus_tag="KPK72_001754"
     CDS             15620..17791
                     /locus_tag="KPK72_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488977.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            17997..18422
                     /gene="ruvX"
                     /locus_tag="KPK72_001755"
     CDS             17997..18422
                     /gene="ruvX"
                     /locus_tag="KPK72_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG2488978.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGIKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18500..18787
                     /locus_tag="KPK72_001756"
     CDS             18500..18787
                     /locus_tag="KPK72_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG2488979.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19069..19527
                     /locus_tag="KPK72_001757"
     CDS             19069..19527
                     /locus_tag="KPK72_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2488980.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19644..20381)
                     /locus_tag="KPK72_001758"
     CDS             complement(19644..20381)
                     /locus_tag="KPK72_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2488981.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20598..22274
                     /locus_tag="KPK72_001759"
     CDS             20598..22274
                     /locus_tag="KPK72_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG2488982.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22480..22680
                     /locus_tag="KPK72_001760"
     CDS             22480..22680
                     /locus_tag="KPK72_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG2488983.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22734..23897
                     /locus_tag="KPK72_001761"
     CDS             22734..23897
                     /locus_tag="KPK72_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2488984.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGTVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24108..24530)
                     /locus_tag="KPK72_001762"
     CDS             complement(24108..24530)
                     /locus_tag="KPK72_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG2488985.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24551..25387)
                     /locus_tag="KPK72_001763"
     CDS             complement(24551..25387)
                     /locus_tag="KPK72_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2488986.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25685..26281
                     /locus_tag="KPK72_001764"
     CDS             25685..26281
                     /locus_tag="KPK72_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG2488987.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26311..27030
                     /locus_tag="KPK72_001765"
     CDS             26311..27030
                     /locus_tag="KPK72_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG2488988.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27032..27571
                     /gene="scpB"
                     /locus_tag="KPK72_001766"
     CDS             27032..27571
                     /gene="scpB"
                     /locus_tag="KPK72_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG2488989.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11227 a         2902 c         4992 g         8582 t
ORIGIN      
        1 caatgtattt tcaacatttt tcaaatagct tacaactaat ataaataaag ataaatatcg
       61 tataaaagca actaattttt ctctgttcat taaaggcact cctccaaaaa aataaacttt
      121 ttctatgaaa actagaataa tatcataaaa gaataaagtc aatagaagta tttattaata
      181 ttgatggaaa taaaatcaat ccaaaaactt gaatagtaaa attgtatact gaccataatt
      241 taaaagtgaa aatttaatgt attggagtga ataatgaata ataaagaaaa aggtgatttt
      301 ggagaaaaag tagcagttaa ttatttgcta tcaaaaggag caaagatact agaaaaaaat
      361 tacagattaa agatagggga aatagatata atagcaaaaa tggaagatga aattatattt
      421 gtggaggtga aatcaaggtc taacataaag tttggctacc cttgtgaatc tgtaagcttc
      481 aaaaaaagaa aaaagataat tggagttgca agttattata ttataaaaaa taacttaaat
      541 aacactccca taagatttga tgttatagag gtatatttat tagaaaaaag aataaatcac
      601 ataatgaatg ctttttaaaa aagataatgt ttttggaaag gattaaatat gttaagtatt
      661 attaattcaa gcaacttagt aggaatcgac agctttttag ttaaggttga agtagatgta
      721 agcaatggaa tacctagttt taatattgtt ggtcttccag gcaaagaaat taaagaagca
      781 agagaaagag taaagtcagc aattcttaat agtggatata aatttccaag cacgagaata
      841 gttgtaaatt tatcaccagc agatataaaa aaagaaggag cttttttaga tttatctata
      901 agtataggcc ttttaagaga gttaataaaa aaagatgaaa attacataag agaaagtatg
      961 tttataggag aattatcatt agatgggaaa ataagaaaag taagaggtat attgcccata
     1021 attatgggtg caaaaactca gaacataaaa cgtattttta taccaattga gaacataaaa
     1081 gaaagtctat ttgtagatga aattgatata attcctatta aaagtttaaa agaatgtgtc
     1141 gattttttaa acgaagagat aaaagtcgat aaagtaagga taatgtcttt tttggatgat
     1201 aagtcaagaa aagagaacga agaattagaa aaagataata gttatataga ttgcaaatat
     1261 actaagatta ataatgaaga aagtaaatat gatgaagact ttaaagatgt aaaaggaaat
     1321 tattttgtaa agaggagtgc agaaattgct gctgctggaa atcataatat gtttatgatt
     1381 ggacctccag gctcaggaaa aactatgatt gcaaaaagag taaggacaat tttaccagat
     1441 ataagtatag aagaaatgat agaagtaagt aaggtctata gtatattggg tatgataaat
     1501 gaaactaaag gaataattga taaaagacct tttagagcac ctcatcatac aactacaaag
     1561 caatctttaa ttggaggagg aatggatgca agacctggag aaattgcttt agcacataga
     1621 ggtatcttat ttttagatga gatagccgaa tttgatagaa aaatacttga gactttaaga
     1681 caacctatag aagatggata tgtaaatata tccagagtaa agtacagtgc aaaatatcct
     1741 tgtagagtac tgttagttgc agctatgaat ccatgtccat gtggttatta tatgtcagag
     1801 acagagtgta gatgtagaag taacgaaatt gatagataca taaataaaat atcaggtcca
     1861 ttgttagata ggtttgatat ttttgtagaa gttaattcta taaaatattc agattttaat
     1921 tctttaaaac aagaagaaag ctctcaaaaa ataaagagaa gagtagaaaa tgctagaaaa
     1981 atacagataa atagatttaa aaaggataat ataaaaaata atagtgaaat taaagcttac
     2041 aatttattta aatattgtaa actggaaaaa gaagcttcaa aaacggctga gatgatattt
     2101 agtaaataca acctaagcag tagaagttat acaaaacttt taaaaatggc aagaacaata
     2161 gcagatttag aagaaagaga cttgataaat tcacagtgta ttatagaagc tttttcattt
     2221 agaaaggctt attatagtta ttttaaatag ttttttgagg tggattttat ggaaaaaaga
     2281 gatacttatt tatggttaaa atcaattggt ggaataacaa caaaaacaat tgaaataata
     2341 gaaaatgaaa tagtaaatat tgaagatata tttgattttt ctgaaaaaga aatatataat
     2401 ttaaaaaata taagcttgaa tattagaaaa aatatagtaa aatatagagg tcacgcttat
     2461 ttagaaaata taaaagaact actttacaaa aaagcgatta aatatatttg taaatatgat
     2521 aaagaatatc ctgaaaattt aaagaatata tacaacgctc caaagttatt attctacaaa
     2581 ggtgacatag gtttagttaa taataatttt aatatagcta tagtaggttc aagaaaacct
     2641 acagcctatg gaattaattg tgcaaaaaca ataagttgtc aactatccca atatggagtt
     2701 aatatagtaa gtggattagc tataggaata gatgcatact cgcacatagg ttgtatgagt
     2761 ggaaaatcta aaactatagc agtacttggt tcaggagtag acaatcctct tccaaaacaa
     2821 aatttacatt tatcaaataa aatattagaa aatgggggat tattattatc agagtataat
     2881 attaactcgg cagtagcccc gtaccatttt tctaatagaa atagaattat cagtggttta
     2941 agtgatggag tagttgttgt agaagcagca ataaaaagtg gtgcactaat cacagtagat
     3001 tttgctcttg aacatggaaa aaatgttttt gcaatacctg gtaatataaa ttctcaaatg
     3061 agtagaggtt gtcataaaat tataaaagaa ggtgcaaaat taattgaaaa tatagatgat
     3121 attttaaatg aatataacat atttaatatt attgataaaa aaataaatca aaaatatgat
     3181 aatataagtc taaatgccaa gtctaagcaa ataattgaag ctataaaaag agaaggaaac
     3241 ttgcatatag atagtatttg tgattatact ggtatagaaa taaagtatgt aaattcaata
     3301 atcaatgaat tagtactaaa tgagttagta gtagaaatga ataataaaac gtacagttta
     3361 aatgtataaa ttaagattag gtagtggggg tgtatggttt tatggccaaa acattagtca
     3421 tcgtagaatc gcctgcaaaa gctaaaacta tagaaaaatt tttagggaaa agtcattaca
     3481 cagttaaagc atcagttggt catgtcagag atttacctaa aagtaaatta ggtgtagata
     3541 tagaaaataa ttttgaacct caatatataa atataagagg aaaaggcgat gtaataaaag
     3601 aattaaaaaa agaagctaaa aaatctaaac aagtatatct agcaactgac cctgatagag
     3661 aaggtgaagc tatatcatgg catttagctc atatactaaa tttagatgaa tctgaggatt
     3721 gtagaataga gtttaatgag ataactaagg atgcaattaa aaaagcaatt aaacatccta
     3781 gaaatattga tataaatttg gttgatgcac aacaggcaag aagagtgcta gacagacttt
     3841 taggttatca aataagtcct atattatggc aaaaggttag aaaaggtctt agtgcaggta
     3901 gagtgcaatc tgttacaaca aagttaattt gtgatagaga aaaagaaata aaggcattta
     3961 ttccaaaaga atactggaca atagatgttg aggctaagac agtaaaagac aaagatataa
     4021 ctttgaaatt ttatggtaaa aatgatgaaa agattgaatt agaaaatgaa gaagctgtaa
     4081 atgaaatact aaaagaaatg gaaggtaaag atttaaaggt tgagaagata gaatctaaat
     4141 caagacgtaa atcagctcca aaaccattta caacaagtat gcttcaacaa gaaggagcaa
     4201 ataagttatt ttttactact aaaaaaacaa tgatgatagc acaagaatta tatgaaggta
     4261 tcgacataga aggtgaaggt actgttggtc ttatatcata tataagaact gactctaaaa
     4321 gaatatctga tgaagcaaaa gaaaaagcta agggttatat cgtaaacaat ttaggagaaa
     4381 actactataa aaacattggt gataaaaaag aaaaaaagga tactaaaaag gttcaagatg
     4441 cccatgaggc aataagacca acctcagttg atagaactcc tgagagtata aaagcctcat
     4501 tgagcaaaga ccagtacaag ttatacaatt taatttggag aagatttgta gctagtcaaa
     4561 tggaagattc agtgtttgat atactaaatg tagaatgtaa aataggcgat attgtattta
     4621 aggcaactgg ttcaaaaatg aaatttgatg gttataccaa ggtatataat ttttcagaaa
     4681 gagaagacaa aattttacca agtatagaaa aaggagatat attaaatata gaaaatatat
     4741 tgccagacca acattttaca caaccaccag caagatatac agaagctagt ttagtaaaaa
     4801 cactagaaga actaggtata ggtagaccaa gtacgtatgc tccaacaata gctactatac
     4861 tgaatagaga gtatgttgaa aagcaaggaa caagtctatg tccaacagaa cttggaataa
     4921 tagttactga tattttggaa aataatttcc ataaatttat agatgtagat tttactgctg
     4981 atatggaaag taaacttgat gttatagaag aaggcaatac agattggaaa aagattgtat
     5041 cagaatcata tgcaccactt aaagaggcta ttgaagaagc tcttgaaaat atagaaaaag
     5101 taaatatgga tgaagaaact gatgagattt gcgagaactg tgggtctaat atggttatca
     5161 aatatggtag atttggaaag tttatggctt gtaaaaacta tcccgattgt aaaacaacta
     5221 gaccaattat gaataaaatt ggtgttaaat gtccaaaatg tgaagaagga gatattatac
     5281 ttagaaaatc taaaagaggt aaagcttttt atgggtgttc taattatcca aactgtgact
     5341 ttgtagcttg gaataagcca acaggagagt tttgtgagaa gtgtggttca tatatggttg
     5401 aaaaagtgac taaatctgaa acaaaaacag tgtgttctaa taaagaatgt aataaagaga
     5461 caaaagagca taatgacgga aaagagtgaa aattgaaaaa aatcacataa ttctcttgaa
     5521 aaaggttcca atctatgata atctttcaag tgtaaacaac taatagaaaa ataaattttc
     5581 attaaatgtg aaaataatat tagaaaatat aaaaggttat taaaaggcct aaatttcatg
     5641 aggagatgat taaatggcaa gtgaagtgtt acaaaaaaca aggaaaataa ataaaacatt
     5701 acaaacaagt ggtggaagca gtgtctcttt tgatttactg gccggagcat tgggcgacgt
     5761 tttaagttct aatgtttatg tagtaagtgc aaaaggtaaa gtactaggtc ttcatttaaa
     5821 tgatgttcaa gacagttcag ttatagaaga tgagtatact aagcaaaaga aattttcaga
     5881 tgaatatact caaaatgtgt taaaaattga tgaaacatta gaaaatttaa atggtgagaa
     5941 gatattagaa atctttcctg aagaacatgg aagattacaa aaatatacta cagtagttcc
     6001 aatattagga agcggtcaaa gattaggaac attggtactt tcaagatatt caaattcatt
     6061 caatgatgat gatttagtaa tagctgaata cagtgcaact gttgttggtc ttgaaatatt
     6121 aagagcaata ggtgaagaat tggaagaaga aatgagaaag aaagctgtag ttcaaatggc
     6181 aataggcact ctgtcctact ccgagcttga agcagttgaa catatttttg ctgaattgga
     6241 tggaaaagaa ggtctacttg tagcaagtaa gatagctgat agagttggta taactaggtc
     6301 tgtaatagta aatgcactta gaaaatttga gagtgcaggt gtgatagaat caagatcatt
     6361 aggtatgaaa ggtactcata taagaatact taatgacaaa cttacagatg aattaaaaaa
     6421 attaaaaaac aatcaataat ctatatttta taggtttaga ttagaaaagg tatctatttc
     6481 aaatagatac cttttttaga cgaattatta aggtaatgat aagtatatta aaaaggagtg
     6541 agaaaatgcc tttagcagat aaaataagac caaaaacaat gaatgatgta ataggtcaaa
     6601 gtcatataat aggtaatgga aaaatattgt ctaagatatt acaatctaat tttttaccaa
     6661 atatgatatt ttttggtcct cctggtgttg gtaaaactac agtagcggaa ataatagcag
     6721 aaagaagcaa taaaagcttc tataaaataa atgctactaa ttcatcttta gaagatataa
     6781 aaaaggtgat atcagagctt ggaagtataa ataatgtaaa tggagtccta ttatatatag
     6841 atgaaataca aagttttaat aaaaaacagc aacaatcaat attagaattt atggaaaatg
     6901 ggagtataac tttgattgct agcactacag aaaatccata tcattatgta tataaagctc
     6961 ttttaagtag gtctactgta tttgaattta aacctttaga aaaacttgat atagaaaaag
     7021 gactgaaaag agctgttgaa gtattaaatg aagatagcta tatggatatt gagtgcaata
     7081 ataatgctat tgaagatata gcaattcttt ctgatggaga tatgagaaga gccttaaata
     7141 ttttggaagt ggtagtctat tcaactaaac caaataagga taatataact tatatagatt
     7201 cagatgtaat aagggcatct acatttaata aaattataaa ctatgacaaa aatggtgata
     7261 gtcattatga tattttaagt gcatttcaaa aatctataag aggaagtgac ccacaggcat
     7321 ctatacacta tctagcaaga cttataaaag gtggggattt gataagtata tgtaggagac
     7381 ttttagtaat agctagtgaa gatataggtt tagcttatcc aaatgctatt gtaattgtaa
     7441 aggcatgtgt agactcagct atgcaacttg gatttccaga agcgaaaata ccattagcag
     7501 aagctactat tcttttggct acatctccaa agtctaattc tgcatgtata gcaataatga
     7561 aagctatgga tgaactggat aaagagtttg ttaaggatat accaaatagt ataaaggatg
     7621 ctcactattc tggttctaaa gaaataggta gaggttgtgg atacaaatat cctcataatt
     7681 ttaaaaatca ttatgtaaag caacagtatt taccagatag cataaaagat agtatttatt
     7741 acataccaca agaaaataaa actgagaaaa atataaaaaa atatttagaa tatctaaata
     7801 gtgattttta gactgaactt tatgtatcgg gggtagatat tatgattata aaacttaact
     7861 caacttatca cagtagagtt atgaaatact taaaaaaaga acctgagtac aatttgttta
     7921 taatagggga tattgaaaga tatggatatg gaaataattt tttaaatata tgggcagatg
     7981 tgggtgaaca tggtgaaata aaggcaatat tacttaaata ttttgaattt atgatgtttt
     8041 attctgatgg agagtatgat gttgaaggtt tttataatct acttcgtaat actaattacg
     8101 aagaaatatc tgggaaaata tgtgctgtag atgctttagc aaagagatta ggacttaata
     8161 atttaaaggt agttgatttt tgcaaacttc aaactaaaaa gtttttgata gataataatt
     8221 gtaatgcaaa ggttaagaga attaggcttg gaaatctaaa aaagacagtg aaactttatg
     8281 acttaataga tgaatttcac agtacaactt tagagaattt aaaaaatgga ctaaggacag
     8341 gtagaggata ttgtatagag attaacaaac aggtggtgtc aatggcaaag agtacttcag
     8401 aaaataggac tcatgcaatg ataattggag taggaacaca tcctaaatat agagctaaag
     8461 gacttgctac aaaatgccta attaaactat gttcagagtt actgagagaa aacaaaattc
     8521 cttgtttgtt ttatgataat gaagaggctg gaaagatata taaaaaacta ggatttgaaa
     8581 acattggaaa atggggaatt tattcaaaat aaaaaattaa aattaatctt gacaaaatta
     8641 gtagggtata ataaaattat cttgaaagta attcctacta aaacagtatg aattagattt
     8701 ctatatagag gtgataatat atgaagttgt ctactaaagg tagatatgga ctaaaagcaa
     8761 tgtttgaatt agcattaaat caagacaatg gaccagtatc attaaaattt attgctaaga
     8821 agcaaaaaat atcagatcag tacttagaac aaatattttc atctttaaaa aaatcgggtc
     8881 tagtaaaaag tgtgagaggt gctcaaggag gttatttgct ttcaaagaat gctgaagaca
     8941 taacagtagg agatatactt gtagtattag aaggaccagt tgcattatct gactgtgtac
     9001 tagatgaaga tgtatgtgaa aactcaaaca tgtgtgtaac aaaaatagtt tgggaaaaga
     9061 tgaaaaaggg tatagaagat gttatagatt caattactct taaagatatg ataaatgact
     9121 ataacaaaaa taaattagaa aatgatataa caaatattaa aaaatagatt tggagatgaa
     9181 tgtaatggaa aaaagaagat tgtatatgga ttattctgca acaacaccta ttaaaaaaga
     9241 agtattggat gcaatgatgc catacctaac agattatttt ggtaatgcat ctagttttca
     9301 cacattcgga agagaagcaa aagatgcttt ggataaagca agagaacaag tagcagccct
     9361 tataaatgct gaacctagtg aaatatattt tacagcaggt ggttcagaaa gtgataactg
     9421 gacactagag ggcgtagctt atgctaataa aaacaaaggt aatcatataa taacttcaaa
     9481 aatagaacac catgcaatac ttcatacatg tgaatactta gcaaaacatc atggatttga
     9541 aataacttat ttagatgttg atagcgaagg aaaagtagac ttaaagcaac tagaagattc
     9601 aataaaagat actactatac ttataagtat aatgtttgca aataatgaaa taggaactat
     9661 acagcctatt aaagaaatat ccgaaatagc taaaaaacat aagatattat tccacactga
     9721 tgcagtacaa gctacaggta atattccagt agatgttaaa gaattaggaa tagatttgat
     9781 gagtatgtct tcacataaaa tatatggacc aaaaggtgta ggagctttat acataagaaa
     9841 aggtgtaaga cttcataact ttgtccatgg tggagcacaa gaaaaaagca aaagagctgg
     9901 tacagaaaat atacctgcaa tagttggata tggaaaagca gcagaattag ctaaggaaaa
     9961 tatgcaaaat catgtagaaa ctttaactcg tcttagaaat aagttaatag atggtgtgtt
    10021 agaaagaata ccttacacaa gagtaaatgg tagcttagaa aatagattgc caggaaatgc
    10081 aaactttgca ttccaattta tagaaggtga aggaatactt ttattattag atatgttagg
    10141 aattgctggt tcaagtggtt ctgcatgtac ttctggttca ttagaccctt cacatgtact
    10201 tcttgcaata ggtttaccac atgaaatagc acatggttca ttaagactta cagttggaga
    10261 ttttacaaca gatgatgata tagattatat actagagaac ttaccaaaag taatagaaag
    10321 acttagaagc atgtcaccac tttatgatga tgcgaagaaa caaggtttag taaaatagac
    10381 ataaagataa agcataaaaa atagacataa attgagtagg aggaaaagat aatgcaatat
    10441 agtgataaag ttatggaaca ttttatgaat ccaagaaata tgggagaaat tgataatgca
    10501 agtggtgtag gtgaagttgg aaaccctaca tgtggagata taatgaagat atttttagac
    10561 atagatggag atgtaataaa ggatgttaag tttaaaacat ttggctgtgg ttcagctata
    10621 gcaagttctt ctatggcaac tgaaatgata aaaggaaaaa ctataaaaga tgcattagaa
    10681 cttacaaata aagctgttgc agaagctttg gatggtttac caccagtaaa gatgcattgt
    10741 tcagttttag ctgaacaagc tgtaaaagca gctcttatag attatgctca aaagaacaat
    10801 atccatatac cagaattgga tggatatgtt atagatgatg ctcatgacca tgatgttgaa
    10861 gaagaagaat aaaaataagt gattgtgtta tgagaagttt acaatgatta aaaagaagta
    10921 ctaatgatat aattaggaca taaaaaatag ctgtttttaa aatggctatt ttttattgcc
    10981 taaattaagt attttaactt gatttagagg ttaattagtt aacaaaataa tccataagat
    11041 tcaaataata aagatgatta tttttttaga aaatagaaca agctatattt taaataagta
    11101 caatacttgc tctatatggt ataataataa gtaatgcaaa agatgaggtg aaatttgttt
    11161 atgaataaaa aagtaatgat aggtatgagt ggaggagttg atagctctgt agctgcatat
    11221 cttttaaaac aacaaggata tgatgtcatt ggtgttacta tgaagttgtg gcaagatgat
    11281 gatgttgttg aaattgaagg tggttgttgc tctttatctg ctgttgaaga tgctagaagg
    11341 gtagcaaata aaataggtat accattttat gttttaaatt ttagagaagt gtttaaagaa
    11401 aaagtaatag attattttat agatgaatat ttggaaggaa aaactcctaa tccctgtata
    11461 gcttgtaata aacatataaa atttgatgat ttttataaga aggctagaca aataggttgt
    11521 gattatgtag ctacaggtca ctatgcaaag atagaaaagg atgaaagtac aggaagatat
    11581 ttgctaaaaa aatctgttac agataaaaaa gaccaaactt atgcacttta taatcttaca
    11641 caagagcaac tagagcatac tttattgcca ataggtgact atgaaaaaga tagagtaaga
    11701 gaaattgcta aggaaatggg tatggcagta cacaataagc ctgatagtca agaaatttgt
    11761 tttgttaaag ataatgacta tgcaaattat gtaaaaaaac attctaaaaa gcgtattgaa
    11821 gaaggttttt ttgtagatac taaaggcaat atacttggaa aacataaggg tatattatat
    11881 tatactatag gccaaagaaa agggcttgga attacttttg gaaaaccaat gtttgtaata
    11941 gacataaatc caataaataa taccatagtt cttggagaca atgaagattt atttaaaaag
    12001 gaacttattg ctaaagatgt taactttata tcaatagata cgttagaaga acccttaaga
    12061 gttcaagcca aaataagata ttcagcaaaa ccatcaccag ctactattca tagagtagga
    12121 gaagatacta ttaaaatagt ttttgatgaa gctcaaagag ctattactaa aggtcaatct
    12181 gtagtaatgt atgatggaga tattgtagta ggtggaggta taatcgagaa aagtttataa
    12241 aaacatctaa aaaattaaaa aaataaacaa taaagtatat taagagtaaa taaagtttat
    12301 aaaataaatt tattgtatat gaaaatatag tattgattaa ttttgtaaaa tttagtaata
    12361 taattagtat attaattaaa aaacaaatac agtgataagg aatagtaaaa tatttgatgt
    12421 cttacagaga gagggaaatg ctggaagccc ttagaatatg atatttgaag cagcctttta
    12481 gtagcaatct tgaacaattt atcttttata aagaagattt aagtagagat tgacggtagt
    12541 tacgttatag ctaaggtttt ccgaagatat catactttgt atgataatta gggtggtacc
    12601 gcgattgtcc tcgcccctat aaatttattt atagggacga ggttttttat ttaaacgaaa
    12661 aaataaaatt tattaggagg aacatgatat ggaaaagatg ggattaaatg aaataagaag
    12721 taagttttta gaattttttg agtcaaaagg tcattatgta gcaaatagtt attctttagt
    12781 accaaacaat gataagagtt tgttacttat aaactcaggt atggcacctt tgaaaaacta
    12841 cttttcaggt gtagaagtac ctccaagtgt gagaatgtgt acatctcaaa aatgtataag
    12901 aacaggagat atagaaaatg taggtataac tgcaagacat gctacattct ttgaaatgat
    12961 gggtaacttc tcatttggag attactttaa aagagagtca atcaaatggg gatgggaatt
    13021 tgttacagaa tggctaaata taccagaaga taagatatgg gtgactgtat atgaagaaga
    13081 tgatgattct tatgatatat gggcaaaaga aatgaatttc ccagaagaaa gaatggttag
    13141 acttggaaaa gatgataatt tctgggaaat aggtacaggt ccttgtggtc cttgttcaga
    13201 aatatatttt gatagaggag aagaatatgg atgtgacaat ccagactgta aaccaggctg
    13261 tgattgtgat aggtatttag aattttggaa ccatgtattt actcaatttg atagagatga
    13321 agaaggaaat tatagtctat tagaaaacaa gaatatagat acaggtatgg gtcttgaaag
    13381 aatgggatgt attatgcaag gcgtagacac tatctttgaa gttgatacaa ttaaatctat
    13441 actagaagca gttgagaagt taacaggagt aaaatatgga gagaatccaa aaaatgatat
    13501 atcaataaga ataataactg accatataag agctgtaaca ttcttagtta gtgatggagt
    13561 gcttccatca aatgaaggaa ggggatatgt tcttagaaga ctccttagac gtgctgctcg
    13621 tcatggtaag ttattaggag taaaagaatt atttttacag aaactaatag atgaagttat
    13681 aaaagtaaat gataaagcat atccagtttt agttgaaaaa gaaagttata ttaaaaaagt
    13741 agttggaata gaagaagaaa aattcaatga aactatagac caaggaacag aaatattgaa
    13801 ctcttatata gaagtattaa aaaatgaagg aaagactgtc ctaagtggtc aagaagcttt
    13861 taaactatat gatacttatg gcttccctat agacttaact aaagaaatat tagaagaaga
    13921 acatctatct gtagatgaag aagcttttaa tgaagaaatg gaaaagcaaa aagaaagagc
    13981 tagaaatgct agaggaaata tggatggaga aagttggaag gaagaccctc tatcaaaatt
    14041 agaatctaca gttgacagta catttaatgg gtatagtgaa atttatggtg aaggaactat
    14101 tgaagctata gttaaagatg atgagctagt tcaaagtgca gaagaaggcg ataaggtatc
    14161 tatagtacta gataacacta ctttctatcc tgaaggtgga ggacaagttg gagattgtgg
    14221 attgataact aatgaaaact tagttttaga agtattaaat actaaaaaag gtgctaataa
    14281 tagtataaaa catataggta taataaaatc aggaagaata agtaatgggg acaaagtaaa
    14341 gacactcgta gatagagaaa ctagaatgtc agctgcaaga aatcacagtg ctacacattt
    14401 acttcataag gcattaagag aagtattagg agaacatgtt aatcaagctg gttctttagt
    14461 aactccagaa agacttagat ttgatattac tcactttgaa gcgatatcaa atgaagaatt
    14521 aaaagtaata gaagaaaaag taaataatgt tattttatca tctttagaca taaaatgtga
    14581 tattatgaat ataaaggaag ctaaagaaaa aggagctaca gcactatttg gtgaaaaata
    14641 tggtgatgag gtaagagtag tttctatggg agattattct acagaacttt gtggaggaac
    14701 tcacttgaca aatacttctc aagtaggaat gttcaaaata ttatctgaag gtggagtagc
    14761 agcaggtgta agaagaatag aagcaataac aggaaaagct gtttatgaat acctaaaaga
    14821 aagagatgga attatctctg aagtttgtgt aaacttaaaa tctaaagaag ataatttaat
    14881 tcaaagaata agttctttat tagaagaaaa taaaaactta tctaaagaat tgcatgatat
    14941 gaaagctaag atgagtttac aatcagttga ttctattttt gattctaaag ttgaagtaaa
    15001 tggagttaat ttgataacta ataaatttga aggtatggat atggatacac taagagaaac
    15061 agcagataat ctaagagata agcttggttc tggtgtagta gtacttgcaa atgtagtaga
    15121 tgataaagtt aactttgtag ttactgccac taaagatgta ttagataagg gaatacattc
    15181 aggtaatatt gttagagaag ttgcaaaaat agctggagga aaaggtggag gaagacctaa
    15241 tatggcacaa gcaggagcta gtgatgtatc taaggttgac caagctttaa gctatgcaag
    15301 tgaggttatt aagacacaag ttaaataaca ttcactcaag gaggttttta gtttggaaaa
    15361 agatttagac tacactatga aatttgaagg aataccagaa gataggatga gtgtaggaga
    15421 tactattgat tttgtatata aagcattagt tgaaaaagga tataacccta taaatcaaat
    15481 tatagggtat ctcctttctg gtgattctag ctatataact agtcataaaa atgcaagagc
    15541 tataattaaa aaatttgaaa gagatgagat acttgaagaa gtaatcacgc actatctgaa
    15601 tagaaagtag gtcaaataaa tgagaaaaaa gattatgtcg ttgtttgcaa tagtcattat
    15661 gatttttgta tcaactgcta gctatggttt tgctgaagaa aaaggcaagg tcatatttat
    15721 tgatatgaat aggaccaatc ttggaagtat gatgaatatt ccaatcttga aaaatgagat
    15781 agaaaagaga ggctatgtag ctctcatgaa tataagaggg gatcaaggta ctgatgatag
    15841 aaggtcttat gcaagtatgg gtgcaggaac aagagctagt gtaactactg aagactatat
    15901 caattttcaa aaagtagacc aagatactgc taaagctttt gaagtcgcaa caggtcaaaa
    15961 agctaaagaa ataaatgatt taggcattaa tagatctata aatgaaaatc ttgagtatgg
    16021 agaatatgga gcaaatttag ggtcattagg aaaaacttta tcagaaaatg gtttaaagac
    16081 atctgtaatt ggaaatgctg atataattga aaataatcaa ttagttaaaa acagaaactt
    16141 atgtttagta gctatggacc aatatggaag gattgattca ggtaatattg ataatattaa
    16201 taagaaagat tctaaaatgc catttggaat atctacagat tatgataaat tactagatga
    16261 aactaaaaaa tcctataaaa atagtgatgc tatatttgta gaacttggag atacatatag
    16321 attagatatg tatagattaa atctaaatga taatacttat gatacaatga agaaaaatat
    16381 tgtaaaaaat ataaataaat atcttgaaca agtattttct atggttggag aaaatgacac
    16441 tgtttatata gcaagtgcat ttccaagtga cctagattat aagaataaga gaagactttc
    16501 tccaataata aaatttagtg gagaagggaa aggtctactg gaatctgcca caacaagaaa
    16561 ggctggaatt gttgccaatt tagatgttgg agtagatata ctaggtacat ttggtcttga
    16621 aaataaagag atggtaggta gaagttatac tttagtaaat aaagaggaca atattagcta
    16681 tctaaaatca ctttatgaaa ggatagtgtc tatatcttca ataaggtcaa ctattataaa
    16741 tggctttgta ggcgtaatat ctgcttcttg ggtaattgcc atgtttgcga ttttctttag
    16801 aaattatatg ccacataaag agcgtgtatt caaagtactt aaggagttta taaaattagg
    16861 cataataatg ccactatcat ttatgttagc tcctttgatg aattttaaaa ctccaatttc
    16921 aatatctttg ggaatatttg caactacgct aatattatac ttgttaggta gaaaattatt
    16981 taaggacaat gatataaagc acatgggatt ttttgcaaca cttactattt tagtcatagt
    17041 tatagattct gtatttggaa catacttaat gcaaaataac ataatgagtt atgatgctgt
    17101 cataggagct aggtactatg gtgtaggaaa tgaatatgaa ggtgtaacta tagcaagtgc
    17161 agtatttggc ttagcagtat tattacatta taagaaggta tctaaattac ttgttgtaat
    17221 tttttcatta ataatactta taactagtgc atatccttca atgggtgcaa atgttggtgg
    17281 agctatatca gaatgtgtag catatttgtt gtttataatg cttatatatg acattaaaat
    17341 agattttaag aaagctgtat tattagctgt ttcagctgta gttgtcgttt ttgcatttgc
    17401 agctttagat atactttcag gtagtgaatc tcatttaggt atgtttgtac agagaatttt
    17461 cctaaatgga ccaggagaga tattacaaac atttggaaga aaaattcaaa tgaatatgca
    17521 actagcacag acaagtgtat gggtaaatat tttacttgta ggaattggag taattgcagt
    17581 gcttatattt aaaccatcta gacactttag aaagataatg aataattttc caatcttgtt
    17641 taaagggttt attgcatcta tggttggttg tataattact cttttagtta atgattctgg
    17701 aatagttgct gcatctacag catctatata tatacttata ccactaataa taataagtat
    17761 aaatatgata atatttgaaa aaaataacta aaaaaatttt tactgttcat ataggattta
    17821 aataaaaaat gcaatatgat tattaatgta aactggatgc ttacataaaa aatcatattg
    17881 caatttttat gattgattat gtaaaattct atgaagcagt aagcctgtga ccttagttat
    17941 gagaggttca gaatatgtat ttaatctata gaatataaat agataaagga ggaactatgt
    18001 tagatggaag aataatgggt cttgatgttg gtgataaaac tattggagtt gctgtgagtg
    18061 atttgatggg attaactgca caaggtataa aaacaataaa aagagttggc aagaaaaaag
    18121 atattgaaga gctaaaagca ataataaaag aaaaacaagt aaataaaatt gtatcaggat
    18181 taccaaaaaa tatgaatgga actttaggtc cacaaggaga aaaagtaatc aagttttgtg
    18241 aattggtaaa agcagaaaca ggtattgatg tagagttttg ggatgagagg ctttctacag
    18301 tagcggcaga gaggtcttta ttagaagcag atgttagtag acaaaaaaga aaaaaggtta
    18361 tagatatgtt ggcagcagta ataattttgc aaggatattt agattttaaa ataaattcat
    18421 aaaagaacta taaaattgaa tacttatgat atataatata aataataaaa ataaaaataa
    18481 atttgaggtg aatgacatta tggaagaaaa tataataaat ttaatagacg aaaatggcgt
    18541 tgaaagccaa tttgaaataa tattaacatt agaagcagaa ggaaaagagt atgcaatatt
    18601 aatgccttta gatgatgaag aagctgaaga agctttaatc tttagaattg atgaggatga
    18661 agaaggtgaa atcttaatac ctttggaaag tgatgaagag tatgaaactg ttgtagctgt
    18721 gtatactgct ataatggaag aagaaggttt aaattacgac gaagatgaat caaatggact
    18781 taactagata agtaattaag tttgtatata aaaatcagat atttattata tttgctttaa
    18841 ttaaacctta ttaattgtaa aagcatttat agtatatatc tgtttttttg ttttttatta
    18901 aaaataccaa aaaatttatg aattttggtg aataatatat cttttatggg tatatattta
    18961 tattatgaga ataaattaaa tttatttaaa tgtcttgaca aaaaatgaat tatcaattac
    19021 aatattattt aataaagatt atcaattgaa aatagtaagg gtgatgttat ggcaaataca
    19081 atggatttat taaaagacaa gttaaaagaa actggtttta agattacgcc acaaagaaga
    19141 gcaatagttg aaatattgct taaacatgac cattcacatc taagtagtga ggaaatctat
    19201 gatttagtta gggttgactg tcctgaaata ggcttagcta ctgtatatag aacaatgcag
    19261 ctattggatg aaataggatt aatatcaaaa ctaaacttag acgatggatg tataagatat
    19321 gagattagtc tacataaaga agactgtcat aatcatcatc atcttatttg taaaaattgt
    19381 ggaaagataa tggaagctaa agaagacctt ttagacaata tagaaaaaga gatacaatca
    19441 ttgtacaaat ttaaaatact tgatcatgat gttaaatttt atggactatg tgacgagtgt
    19501 aatggagtaa gcgattcaga agaataaact tctattgctt aatgtcgatg gaagaatagt
    19561 atagaacttt tatattctat gctattcttc cattttttat tataaaccat ctataaaatg
    19621 caaatataca gttttcaaga tgcttatagt tttaatattg aaaatataat taatcctata
    19681 agtacaaaaa tgaatgattt ttctatgaaa ttactaaatg gtcctccaac agttaaaaaa
    19741 cctaaagaaa ttttcttttc gcttagcgga taaaaaattg gtattccttg ctttgtaaat
    19801 aaatctccta aaaacatatg acttgcataa ccaatcgtac cataaaaaga taaattatta
    19861 atattagtga ttaattctat ctgttttagt aaaaaatata ctattatagt tgcaaatatg
    19921 ctatgagaaa aacttctgtg ctttaaccag ggaaaaattg acaacattag acaaaaaatt
    19981 gtaaaatata tttcaacttc tatgatgtat aagcatatag ctaatagtat aaatattaat
    20041 gatagaaaaa cttttcttag aaatgtgtgg gttagttttg cttcaataat tataattgca
    20101 acaaatgtaa ttattgcgct taaataaaaa ttatcattta tatttattga aataaagaaa
    20161 ataataatat taacagcata tataaaaatt ctatatatgt atttggatac attgtttttt
    20221 agaatgaaat ttgagaccat agaattagat ttatccaaat cgggtagtac agcaaaagta
    20281 gctgaaacta aaatatcaac taaagatata ggaattttaa atattaaaga tgtttggata
    20341 gctgttaaga ttcctattgc acaatgagtt ttaccacgca taaatcctcc taaattatcc
    20401 gatattaaat catataatat ttagaggata tcaaaataaa ataggtttgt caaaataata
    20461 cgttaaatgt attttaaata tagcttaaaa tatgatacga attatggtac aataagaata
    20521 actggtagcg agtgcgtgat ttgatattat ttatgattgt agaacgtgtt aaaaaattaa
    20581 atagacagaa ggagatgatg caattgttca aaaagaacac caacaagata aaggtaatgg
    20641 ccttgggtgg acttaacgaa gttggaaaaa atatgactgt tgttgagtat aaagatgaaa
    20701 taattgttat agatgctgga cttagttttc cagaggatga aatgttagga gtagatatag
    20761 ttataccaga tataacttat ttagtaaaaa atagggataa aataaagggt atatttatta
    20821 cacatggaca tgaagaccat ataggagctc ttccatatat attaaagaaa ataaatgtac
    20881 ctgtatatgg ggcaagactt agtataggcc ttatacaagt aaaacttaaa gaacataaaa
    20941 tgaataatgt taagttaaat gttataggtc ctagacaagt tataaagctt gataatatgg
    21001 aagtagagtt tttgaaaaat aaccatagta taccagatgc ttactctatt gcaatacaca
    21061 cagaccaagg tataatatac cacacaggag attttaagat tgacttgact cctattgatg
    21121 gagatgttat ggatatgcat agaatctgtg agttgagcaa aaaaggagtt cttttaatgc
    21181 tggcagatag tactaatgca gaaaaaccag gatttactat gtctgaaaag actgttgggg
    21241 taggtcttga cgaattattt gcaaaaggaa atggcagaag aattatagtt gctacctttg
    21301 catccaacat acacagatta caacaaataa taaatactgc tgaaaagttt aatagaaaag
    21361 ttgctatatc aggtcgctca atggtcaatg tagttggagt tgcaaaagaa ttaggatacc
    21421 tagatatttc tgatgacatg cttatagatt taaatgatat atgtaagtat gaagatagtg
    21481 aattagtgat aataacaact ggttcacaag gtgaacctat gtcagctctt gctagaatgg
    21541 ctttttcaga gcacaaaaaa gtagaaataa agagtggcga tttagttata atatctgctc
    21601 atccaatacc aggaaatgaa aaattaatat caagagtaat caatttctta tttgaaaaag
    21661 gagcagaagt agtatacagt gatatagctg atatacacgt ttctggacat gcctgtcaag
    21721 aagaattaaa acttatacat gcattagtta gaccaaagtt ttttatgcca gctcatggtg
    21781 aatatagaat gttaaaaaga catgctgaaa tagctgaaca gcttggtatg gataaagaaa
    21841 atatttttgt catgcaaaca ggagatgttt tagaattaga taaaaactct gctaaggtag
    21901 caaaccgtat acaaacagga aacatattag ttgatggttt aggagtaggc gatgtaggta
    21961 atatagtttt aagggataga aagcatttat cagaagatgg tcttatgata gttgtagtta
    22021 ctatttccaa ggatgagggt aaggtcttag caggtccaga tataatttcc agaggattcg
    22081 tatatgtaag agagtcagaa gatttaatgg atggagcaaa ggatataatt aagaatgttc
    22141 taaatgaatg tgaagagaaa aatattaaag aatgggctta tttaaagaat aatattaaag
    22201 aaaacctaaa agaatattta tatcaaaaaa ctaaacgaaa tccaatgata cttcctatca
    22261 taatggaagt ataatataaa aaaactataa atttgaaaat tagacctctt gtaattttta
    22321 aagtaatatc aaaaatccaa ttgatattat cttaagaatt acaagaggtc tttttaatac
    22381 tcatttaaaa aaaaaaaata ttacataaaa taccaattat aacaatcttt tattagtaaa
    22441 aactacttat atataaattt tactgatagg aggattctta tggatgatgt ttcaagacaa
    22501 aatgctataa aagctttaaa acaaacaaaa atggaaatag caggagagta tggaatgaat
    22561 tatgaagatg cttttgaaat aatagaaaat gcgtctaata aaggtgtttt agaaggttac
    22621 tttaaaaagc ttgaaaagaa aaaaaattta ggtcaaggta tatctaggca tttagaataa
    22681 aaaaagttaa atgtgagaaa ataagtttaa ataaacttta agaggtgaca tcgatgaaaa
    22741 aattagctag tactgctttg gctatattaa ttgcgcttac tcctttaagc ttttcttttg
    22801 caaataataa ggaaaatgct gatgccaacc aactaaatat atcttcaaaa tcagcaattt
    22861 tgatggatgt tggtagtgga caaattttat atgaaaagga tgctcataaa aagcttcctc
    22921 ctgctagtgt aactaaggta atgactatgc tacttatagt agaagctttg gattcaggca
    22981 agattaagct tgatgatgaa gttcaagtaa gtgaaactgc atctagtatg ggtggtagtc
    23041 aaatattcct agagccagga gaaactcaaa aggttgatac attattaaaa ggtatagctg
    23101 ttgcttctgc aaatgatgct tgtgttgcta tggctgaaca cttagctgga actgttgaag
    23161 gatttgttga tagaatgaat gctaaagcaa aagagttaaa tatgaatgat actcattttg
    23221 caaatacaaa tggacttcca gttgcaaacc actatacatc tgctcatgat atagctttaa
    23281 tgtctagaga actattaaaa cacgaaatga taagtaaata cttaactaca tggatggata
    23341 aggttgtagt agggaagaaa caagttacag taggtcttgc aaatactaat aaacttataa
    23401 agcattatca aggagcaact ggtgttaaaa ctggttttac tcaagaagca aaatactgtt
    23461 tatcagcatc tgcaaagaga ggtaatactc atctagttgc tgcaacttta ggagcagaga
    23521 cttctccaga aagatttaat gatgcctcta gccttttaaa ctatggattt gctaattatg
    23581 aaagtgttaa gttatgtagt aaaggtgata atatagcaac acttacattg gataaggctg
    23641 atgaaaacaa agttaaattg gttgctaaag aagatttaaa tgctcttatt aaaaaaggaa
    23701 gctctaaaga atttgagaag aaaattgaaa tagtaaaaaa tcctaagatg ccaattaaaa
    23761 agggaactgt tttaggaaaa ataaagatat gtaaagataa aaaagtaata ggcgaagtgg
    23821 aattgataaa tactaaggat ataaataagg caagctatct acaaatgctt caaagaataa
    23881 ttgataatat gatataaata attatcagat gaaatataat tataatacac aaagatatga
    23941 ttataataca cataaaaaat acccttatgt aattatttgc cataggggta tttttattta
    24001 ctatatagaa gtattttata atcttatgat ttttatagaa atatagaaaa gtttatgtat
    24061 cttaaaatat catttgattt aatatcatat atagggttgt ttaacaatta tctaagtaaa
    24121 ggcatactac cagtaagttt attcactttc tttttattcc cataaattgc tatcccaaaa
    24181 tactgtaact catcttctgg aacactagca atcttttgtg taaattcttc ataaagattg
    24241 caactttgtg ctacatctga aaaatctaca acagttaaat cgttaaattc caaagtatat
    24301 aattttttac gtaaatcttt tattatttct ttgtttcctt ttaaaatagg tacaggaatg
    24361 tcaataattc ctaagtggct attacctgtt ttgtcaatta catctggccc aacagtttct
    24421 ggggcatgtt ttcctagtgt aattcccatt atagctgctg tattggatat aattcccatt
    24481 ggtaaatttt cgtctattac catgacacat tttgaatttt tattttccat ttttatttct
    24541 cctttttacc ttatttaaag ttgtttgaat catcatttat aaatatattt tgatactgct
    24601 ttggtgtaag accaataaaa ttcttaaaaa agtttgtaaa gtgactttgg tctacaaatc
    24661 ctgtttgtat cgcagcatct ataggttcta cgcctttttc taaaagcttt ttagctttgt
    24721 ttacccgtat attttctagg tatctatatg gtgtaatacc ttttaatttc gtaaaattac
    24781 gaagtagaga gtacttattc atacctgcta tagtgcttaa ttcgtcaagt attatatgtt
    24841 cagcataatt attttctaga taatcacata ctgcttgaat ttctatattt gtattttcca
    24901 agttagattg caaatttggc tctgtatgtt cttcaattaa ttgttcaatt aggaagaaaa
    24961 aaagctcttc ctttttaaaa tccaattctt cttccatgat tatatagtgt aattcttgta
    25021 gtagaggtac taactcactg cgaaatataa caggttgatt aaattttggt aaataatttt
    25081 tacctgtaat ttcaaagacg gtttttttca ttatttctgg cttaatatta atacatctgt
    25141 aatcaagtac tttatcatct atttgttcac aagtatgact atcaaaaggg ttaaaaagta
    25201 gcaaatctcc agtacttgtg gtatactctt tatttttaca agataaatat ctttgacctt
    25261 tttctataaa accaattaca taatgttcat gaaaatggtt tgggaatttt tgcattatgc
    25321 ctttaaatct atatgcttca atttttaatt ccctatcaaa gcatactgtg cgttcttctt
    25381 gtctcactta ggtaacctcc tttcatgtta tacaaagtgt aacacatatt tttttatact
    25441 tcttgtataa tattgactaa gagacaataa tgtcaattca tgtcaatata atattacact
    25501 tacagtaggt gtaattatta tattttcaca atgaatatga tataataata aaagttataa
    25561 aataaaaagc aaattttaat tttatgccta gaagttaaaa ttattataat gtaaaataat
    25621 tttgattata agctatttaa ttgttataat aatattaaaa atttaattta ggagatgtac
    25681 attaatgtta agtatgaata taggagaaat agtagcaagt ttagttggta tagctatggc
    25741 aatatcaata catgagtttg gccatgctta ttcagctcac ttattgggag atgatacagc
    25801 taaagcttat ggtagaatga cattaaatcc tgtaagacat gttgacccaa taggtctttt
    25861 ggctatgttt atccttaaga tagggtgggc aaagccagta ccagtaaatc caaataactt
    25921 taaaaactat aagataggaa acctgatagt ttcacttgca ggcgtttttt gtaatgtttt
    25981 gactgctata ctgtgtgtaa ttatcaataa gtatgtgcat atgtatgcta taaatacaat
    26041 agctggatat gtgcttttat atagtatggg atttgcagct tttaacttac ttccgatacc
    26101 tccacttgat ggatggggag ttatatctac atttgtacca tataaatgga atgaatattt
    26161 gtataaattt gaaagtatga gttatataat tttattaatt gctttattta ctggtatgta
    26221 tagaataata ttaacaccaa tatatgcagt gttgctaaaa atagtatatt tatttgttta
    26281 atataataaa cagattaaag agggattgat atgaagtata atatacaatt acaagtctat
    26341 gagggaccat tagatttact ttatgattta ataacaaagc ataaaataga tataaaggat
    26401 atatccataa tagatataac taagcagtat ctaaattatt tgaaaatgct agataaaatg
    26461 gatttagaga taactagtga atttataact atggcatcaa aacttttaga gataaagtct
    26521 aaatatctgc tttataagca aaaagatgaa gaagaagacc caagaataga acttatggaa
    26581 aagttagaag aatatagaaa gtttaaggta gcatcacaag atataaaaga aaatataact
    26641 tatgttaatg agagatttta tagaaataaa gaagagataa ttattgatga caatgtggat
    26701 ttagaagata tatcaattga agcaataaaa aatatacttc catacatatt taaagttaaa
    26761 acatcacaga tagaaaatgc taatgatgag aaactagata aaatagtaag gaagaaaata
    26821 atatctgtag aagaaaaaat attatatata agagatatta taaaagagaa gattgaagtt
    26881 acttttacaa atattataaa atcatatgaa aatgatgaaa taatagctac ttttttatct
    26941 atattagagc ttataaaaga aaaagaaata gtcgtagtac aagatatatt ttttgatgat
    27001 attctaataa gaaaaagttc ggagtgttaa aatgaaaaga gaagatataa agtatattat
    27061 agaatctgtt atgtttgcat atggtgagcc aataagtata aaagaattaa actatataat
    27121 aaataaagaa ctttcatcaa aagaaataga gattatgtta aatttactta ttgaggagta
    27181 cagagaacaa aatagaggta ttcaaataat caagttagag aataagtacc aaatgtgtac
    27241 aaataaagac tatgcagaat atataaagaa gattattgaa ccaaagaaaa agaaaagttt
    27301 gagccaagct acattagaaa ctcttactat aattgcctac aaacaaccaa taacaaaggt
    27361 agaaatagag gatataagag gagtaaaatg tgataaggta ttacaaactt tgtttgaaaa
    27421 tgaacttata agagaagctg gtagattgaa taagataggg aaaccaataa tttataaaac
    27481 aacagatgaa tttttaaagt tgttgaatat agaaagtctt gaagaattgc cacctataga
    27541 aaattatcaa gaagtggcaa caaatgaata aaaatataaa atcacaataa tttagtatgt
    27601 aattttaaat tttaaaagat ttaaaatgct ctaagttgtt gtgttttttt gtataaatat
    27661 aaaattataa ttctcttaaa ataaatatgg ataattaagt ata
//
LOCUS       DAEMHZ010000044        27635 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000044 DAEMHZ010000000
VERSION     DAEMHZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.044"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..1555
                     /locus_tag="KPK72_001767"
     CDS             191..1555
                     /locus_tag="KPK72_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2488990.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1577..2068
                     /locus_tag="KPK72_001768"
     CDS             1577..2068
                     /locus_tag="KPK72_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2488991.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2341..3012
                     /locus_tag="KPK72_001769"
     CDS             2341..3012
                     /locus_tag="KPK72_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG2488992.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3097..3963
                     /locus_tag="KPK72_001770"
     CDS             3097..3963
                     /locus_tag="KPK72_001770"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG2488993.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3996..5498
                     /locus_tag="KPK72_001771"
     CDS             3996..5498
                     /locus_tag="KPK72_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2488994.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5628..6080
                     /locus_tag="KPK72_001772"
     CDS             5628..6080
                     /locus_tag="KPK72_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG2488995.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6240..7043
                     /locus_tag="KPK72_001773"
     CDS             6240..7043
                     /locus_tag="KPK72_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2488996.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7101..7988
                     /locus_tag="KPK72_001774"
     CDS             7101..7988
                     /locus_tag="KPK72_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2488997.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            8007..8855
                     /locus_tag="KPK72_001775"
     CDS             8007..8855
                     /locus_tag="KPK72_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2488998.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8918..9607
                     /locus_tag="KPK72_001776"
     CDS             8918..9607
                     /locus_tag="KPK72_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2488999.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            9998..11005
                     /gene="asrA"
                     /locus_tag="KPK72_001777"
     CDS             9998..11005
                     /gene="asrA"
                     /locus_tag="KPK72_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG2489000.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            11006..11797
                     /gene="asrB"
                     /locus_tag="KPK72_001778"
     CDS             11006..11797
                     /gene="asrB"
                     /locus_tag="KPK72_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG2489001.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGK
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYCGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            11810..12781
                     /gene="asrC"
                     /locus_tag="KPK72_001779"
     CDS             11810..12781
                     /gene="asrC"
                     /locus_tag="KPK72_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG2489002.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVVLTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            12838..13614
                     /locus_tag="KPK72_001780"
     CDS             12838..13614
                     /locus_tag="KPK72_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2489003.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            13885..15903
                     /locus_tag="KPK72_001781"
     CDS             13885..15903
                     /locus_tag="KPK72_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2489004.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            16118..16975
                     /locus_tag="KPK72_001782"
     CDS             16118..16975
                     /locus_tag="KPK72_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2489005.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            17072..17941
                     /locus_tag="KPK72_001783"
     CDS             17072..17941
                     /locus_tag="KPK72_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2489006.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(18312..18944)
                     /locus_tag="KPK72_001784"
     CDS             complement(18312..18944)
                     /locus_tag="KPK72_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG2489007.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(19016..19369)
                     /locus_tag="KPK72_001785"
                     /pseudo
     CDS             complement(19016..19369)
                     /locus_tag="KPK72_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(19447..19650)
                     /locus_tag="KPK72_001786"
     CDS             complement(19447..19650)
                     /locus_tag="KPK72_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2489008.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(20040..20270)
                     /locus_tag="KPK72_001787"
     CDS             complement(20040..20270)
                     /locus_tag="KPK72_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489009.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(20272..20679)
                     /locus_tag="KPK72_001788"
     CDS             complement(20272..20679)
                     /locus_tag="KPK72_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2489010.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(21039..21488)
                     /locus_tag="KPK72_001789"
     CDS             complement(21039..21488)
                     /locus_tag="KPK72_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2489011.1"
                     /translation="MELIEWNYTYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTYINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRESSKVLEKCGFKIEEYCHNS"
     gene            complement(21502..22428)
                     /locus_tag="KPK72_001790"
     CDS             complement(21502..22428)
                     /locus_tag="KPK72_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489012.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEGNNTIPIHMATTTRVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(22553..22957)
                     /locus_tag="KPK72_001791"
                     /pseudo
     CDS             complement(22553..22957)
                     /locus_tag="KPK72_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(23107..23727)
                     /locus_tag="KPK72_001792"
     CDS             complement(23107..23727)
                     /locus_tag="KPK72_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2489013.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(23826..25094)
                     /locus_tag="KPK72_001793"
                     /pseudo
     CDS             complement(23826..25094)
                     /locus_tag="KPK72_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            25186..26001
                     /locus_tag="KPK72_001794"
     CDS             25186..26001
                     /locus_tag="KPK72_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBG2489014.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSTNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            26012..26161
                     /locus_tag="KPK72_001795"
     CDS             26012..26161
                     /locus_tag="KPK72_001795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489015.1"
                     /translation="MTRVYYLFTTFDGILIRLNANLCKVSSKKKMSILLVDTGYIDIY
                     QLMKR"
     gene            26673..27440
                     /locus_tag="KPK72_001796"
     CDS             26673..27440
                     /locus_tag="KPK72_001796"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG2489016.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT        10547 a         3187 c         4649 g         9252 t
ORIGIN      
        1 tttaaattaa gcataataaa atataaataa taaagttcgc attaacaaac tatttaaata
       61 tcaagacctg ttaaaaaaca acagcaataa acaaataaaa tagataaata tatccaatta
      121 aggtataatt aagttttata tgatatagtt atatttgata atttacaata gtaaaacttg
      181 gagggaaaaa atgggtgaaa agaaaataaa taatatgaat tattcacata ataattattc
      241 caaaaagaca ggtgaaccaa ctagaaaaat aaaaaatagc catatcactc ttaaaagtaa
      301 agaagaaaaa atttgttcta gttgcaataa agaaaataat actaaggata attactgtaa
      361 attttgtgga aatgaacttt atgaaatagc atcattaaga ccacttgaaa caaaactaga
      421 cttgaaatct aaaataaaag agctttcata tcatgcaaat aaaagaggtg tatttttaac
      481 aacatttaca accatattta tattgtttat tattgcattg atatttaaag ctattataac
      541 tattcaattt aatgatatta gttatttaat aaatccagca catataatcc tagctctcaa
      601 cttgggtcaa attagtgtat ctatgtctac catgatgggt tctgggttta taaatgccaa
      661 tataggtttg ttgatattac ttataatccc tattttggta ttattaatat caaacctaat
      721 ttttatgaga aaaagatgta gagattcaaa aactgtatta gcaaattctc taggagttgg
      781 aattttttat ggattagttt tagctatttt atctattttt actaatgtaa aaactagttc
      841 acatagtatg ttagaatatg gatatgcatt agaatatagt tatgaatttt ttagtgttct
      901 tttaaatgga ttcgtattag ggtttatatg tacatacata actacttata aaaaaagtta
      961 tgaaaaggaa aatatgtatt tatctctttt ttcaaattct ataagaacat tcatattagg
     1021 ttatgtttta gtattagtaa tactattggt actaactata tctgatagta gttacttaaa
     1081 tgaactagat atgagtagtt attcaaatgg attaaattta tttactatac tcccacaaat
     1141 agctagttat atgtgggctt ttgcaaatgg aatttcagta accataataa actcaacagt
     1201 atcaatgttt actttaagtt cttcatcttt atttggagat actaaattaa tgttttatgc
     1261 aatgggagca ttgtctatgt taatattatt attaaatgga tacaagctaa gatttaagta
     1321 taatacagat agtataagac ctataatagt atttagtata tattatgcat ttttgatggg
     1381 aatattggct ttatttagta catttatact agatagtaat attaatttct ttaatacaac
     1441 taattatgga acaactttaa taatgcaatt taaagtctta caagctattg taatttcatt
     1501 tgtatatagt tttgtgatat ctttaatagg atataagcta aattcagctg attaaatatg
     1561 attggaggga atttctgtgg aaggaaatac taataataaa gtagatgata aattttctaa
     1621 ggttaagaaa aagtttggta gtggaaaatt taaagttgaa ttatataaag aaaaatcaaa
     1681 attaaataga gagattgatg atattcaaga aaagaaagca aagatattct tggaaatggg
     1741 aatactgaca tatcaaaaaa ttagagaaga ttctattaat gataatagtt ttgataaatt
     1801 ctgtgatgag ttactagaac ttgataagtt aatttatgaa aaaaatatgg aaattaatga
     1861 aatggaaatg gcagaaagta atgtaacttg tgagtgtggt tatgttggaa atgtaaatga
     1921 taaatattgt gctgaatgtg gtaaaaaatt tgaattggaa aatgaatgtg atgattttat
     1981 aatttgtggt tattgtgaaa gtgagataga tacagaggct gaattttgtc catgctgtgg
     2041 aagaaaaatt ataatggatt tagaataata ttgggaaacc tatgtatagt atacatgggt
     2101 ttttttattg aaattattta aatggactta taacagtagt tatataaaat gaaaataaac
     2161 accaaaaaaa tgaaataaat tttcttttaa atataaaaaa caagctaata tataaaataa
     2221 atcagtaaaa atatagatgc gagaaaaaaa tatgaaaaaa tattgcaaag aaaaagtttt
     2281 catgttatta ttaatacata aatcaagaaa ataaatttca gaaacgaggt aatttaaaaa
     2341 atgctagata aggtaaaggg aagattaata gtatcatgcc aagctttaga aaatgaacct
     2401 cttcatagtc cttttataat ggggagaatg gctaaggctg ctatggaagg tggagctgtt
     2461 ggaattagag ctcaaggagt tgaagatatt attgaaataa aaaaagttac tggacttcct
     2521 gttataggta ttataaagag aaactatgaa gattcagata tatatataac gccaactaaa
     2581 aaagaagttg atgaactttt aacaacaggg tgtgaaatga tagcattaga tgctaccaat
     2641 agagttagac caaataatga agacttaaaa gaacttataa agtatataaa agaaaacggt
     2701 gttctagtaa tggcagatat ttcaaattat gatgaggcta tcaaagcaca agagtatggt
     2761 gtagattgcg tttcaactac actatctgga tatactccat atactaaaac attagaaggt
     2821 ccagattttg tattaatgga aagattagtt aaagatttag aaatacctgt aatagctgaa
     2881 ggaaaggtaa atactcctca agatttaaaa aaggtctttg agttgggggt tcattcatca
     2941 gtagtaggtt cagcaataac aagaccacaa ttaataacag aaaaatttgt taaagcaatt
     3001 gaaattaatt aaaaattaat ttataaatta aaaaattatt atatttaact atatttgaaa
     3061 attaaaattg gaggtaaaga tatgagaaca acagatatga agggaattta ttcagcatta
     3121 ttagtttcat ttgataagga aggaaatata aatgaaaaag ggttaagaca aataattaga
     3181 cacaatatag atgtttgtaa agtagatggt ctttatgtag gaggaagtac aggggaaaac
     3241 ttcatgcttt caactgatga aaagaaaaga atatttgaaa tagctaaaga tgaagtgaaa
     3301 gaagaaataa aattaatagc tcaagttggt tcagttaatt taaaagaagc tgtagaactt
     3361 gctaaattta caactgattt aggatatgat gcaataagtg ctgttacacc tttttactat
     3421 aaatttgatt ttgaagaaat aaaacactat tataatacaa taataaattc tgtagataat
     3481 agattaataa tatactctat accattttta actggtgttg atatgagttt agaccagttt
     3541 ggagaattat ttgaaaatga aaaaataata ggagttaagt ttacagcagc agatttctac
     3601 ttattagaga gaatgagaaa aacattccca aataaattaa tatttgcagg atttgatgaa
     3661 atgatgttac cagctacagt tttaggtgta gatggggcaa ttggttctac attcaatgta
     3721 aacggtgtca gagctagaca aatatttgaa ttaactaaga atgaaaaaat atcagaagca
     3781 cttgaagtac aacatgtgac taatgattta ataacagata tattaggaaa tggcctatat
     3841 caaacaatta aattactact tgaagaacaa ggtgttgagg ctggatactg tagacaacct
     3901 atgaaagaag caactgatga aatgaaatca agagcaaaag aaatatacag aaagtatttt
     3961 taataaaata cttttagtat gggaggatta gtcaaatggt aggatttact tggatagatt
     4021 ttgcaatact agtggtttac ttattggcag ttttatttgc aggattgtta ttttcaaaaa
     4081 aggaaatgaa gggaaaagag ttcttcaaag gtgatggaac tattccgtgg tgggttacat
     4141 cagtatcaat atttgccaca ttattaagtc caatatcatt tttatcatta gcaggaaatt
     4201 cttatggtgg tacttggatt ttgtggtttg cacaattagg tatgtttata gcaatacctt
     4261 tgacaataaa atttttctta cctctatata gtaggttaga aatagataca gcatatgaat
     4321 acttagaaat gagatacgaa agcaaaggtc ttagagtact tggagctttg atgtttataa
     4381 tatatcaaat aggacgtatg tctattataa tgtatcttcc atcaatagtt ctggctggtc
     4441 ttacaggaat acctgtaaat gtactgataa tagttatggg tgtaatagca ataatttact
     4501 catatacagg tggattaaag gctgtcttgt ggaccgattt tatacaagga atggtattaa
     4561 tagtaggtgt tacagggaca ttgttctatt taatagcaag tataaatggt ggttttggaa
     4621 cagtaatgga tactcttaca actggacaca agttcttagc aagtaatgaa gttatgtttg
     4681 ataaaaatat actatctaca agtgcattta taatatttgt tggagctggt cttaatactt
     4741 tttcatcata tgtatcgagt caagatatag ttcaaaggtt tactacaaca actgatataa
     4801 agcaacttaa taaaatgaca tatggaaatg gtgttttatc aatgggactt gcaacagtat
     4861 tttatctaat tggaacttgt ttatttatat actatactca aaacccagat ttagctcaaa
     4921 cagttcaaca agaccaagta tttgcatcat atatagctta tgaattacca gtgggtataa
     4981 caggaatact acttgcagcg atatatgcag cttctcaatc aactttatcg acagggctta
     5041 attcagttgc tacaagttgg actttagata tacaaactat cataacaaaa gatataagta
     5101 tggaaaagca aactaagata gcacaatata tatcattagg tgtaggtata ctttctatag
     5161 ctgttgcaat agtattagca aatggagaaa taaagtcagc atatgaatgg tttaatagtt
     5221 ttatggggct agtactaggt gttttagcag gaatatttgt acttggtgca ttttgtaaaa
     5281 aagcgactaa aatgggagca tatgttggat tcatagtatc agcaatttta gttgtttatt
     5341 taaaataccg tatgccagaa gtaacttctt gggcatattc tctaataaca atcacgactt
     5401 ctgtagtggt aggtacaata gttagtgcaa tagaagctaa agttaaagca aaagtatctt
     5461 taccaaaagc tgagactact gtttattatg aaaaataata tttaaatgat aaattgtctt
     5521 atagagtgat tagataaata atttagttac tctataagat tttataatat actttgtata
     5581 taaaagtata aaaatagact ttataattat atagggagat agtgcttatg atatttggaa
     5641 atataaatca tagtaagaca tattcaacat tgcatgaaga tttacttaga tgttttgaat
     5701 atgctaaaga taattcatta atagattatg aaaaaggtac ttatacaata gatggtgatg
     5761 atatttttgt gaatattgta gagtataaaa cttgtgaaaa agaagataga ttctgggaag
     5821 ctcataagaa gtatatagat gttcatttga tgcttgatgg atgtgaacga atagatataa
     5881 attttataga aaatttagaa caaaaaatat atgaagaaaa gggcgatttc ttgcctcttg
     5941 atggtaaaaa taatggatac atggaactta gaaaaggtga ttttttagtt tgttatccgg
     6001 aagatgctca tatgacagga ataaaagttt tagaaaaaga aaatatcaaa aaagctattt
     6061 ttaaggtttt ggttagataa tgataattta tataaccact agggctgaaa atagtgattt
     6121 aactttcagc gattagtgag acttcgtaaa ttttagttag attactattt tcagccttag
     6181 ttttttatga gaaaattgaa aataattaaa agcttaaaat tttgtaaagg agttaactta
     6241 tggaaaatat agatgtattc ttattaatag gacaaagcaa tgctagagga ttgggaaatc
     6301 caaaagagag catcattcca aatgaaaatt gttttgaata tttgagtact gatgaaatta
     6361 taaatatgag atgtgagcta gagacttcag aaggtgatgg tactatagca cctgcatttt
     6421 caaatgaatg gaataagctt actggaaata aagtttgttt tattcacaat gcaaaagatg
     6481 gttcaagaat aaaaaactgg aatcatgata ataactggtt tctaaatgac actatagaaa
     6541 aatttaatac aggctgtact accttaagta aacattataa tatagaaaat aaatacgtta
     6601 tttggattca aggtgaaagt gatgcaaaat atggtagtga tgtattgtac tataaagaaa
     6661 gtctaaaaaa aattgcgtat aggcttaaag aagaatgtat gattgataaa atgtttgtaa
     6721 gtttaactgg ttattggctt ggagaagatg aatattttat tagaacaaga cgaattgcag
     6781 cagcacagga gtcggcttgt agtgaatgtg atatattatg tgtaggaagc aaaattgcta
     6841 tgcagtttca tgacagagga ttaactattg atgatgttca ttacactcaa gaaggtctta
     6901 atatgttagg agaggactta tgtaaaaata tttataagta tcatataacc aaagaaaaaa
     6961 gtgtattaag tgatactata gacttatcag aagcaaggaa atatatctgt gaattggaaa
     7021 agataagtaa aagatttgtg taatataaaa ttttaaatag agttgttaat ttataaaaat
     7081 aatttgtatg gagatatgtt atgaaaaatt acgtttgtat agatgttgga ggtacttcaa
     7141 taaaatatgg ttttattaga gaagatggat ttataattga taaatcaagt cttgatactg
     7201 aagcaaagga aaaaggtgga gaaggaatat tagccaaaat aaaagatata gtaaaaaaat
     7261 atatagagga aaatgaaata agtggtatat gtatatcaac tgcaggtatg gttgacccag
     7321 ttgaaggtaa aatactattt gcattagaag aattaattcc aaactataag ggcatgcaat
     7381 taaaaaaaga agtagaaaaa gagtttaata taaaatgtga agttgaaaat gatgttaatt
     7441 gtgctggact tggtgaaatg tggcttggag caggtagagg tgcaacatct tctatatgtt
     7501 taacaatagg tactggaatt ggtggatgta ttataataaa taataaactg ataaatgggt
     7561 ttagtaatag tgctggtgaa gtaggatata tgaatgtaaa tggaagttct tttcaagaat
     7621 tggcatctac tagcagtcta attaaaaaag tagcaaaaat aaaaaatttg aatgaaaatg
     7681 acttaaatgg aaaaataatt tttgatttag caaagaataa tgatgaagat tgtttaaaag
     7741 aactcgataa tatgataaaa tcattagcag ttggaatcgc aaatatatgt tatgtaataa
     7801 atccagaagt ggtaatttta ggtggtggga ttatggcaca agaaaagttc ctaaaaccaa
     7861 aaatagacaa agcattaaag gaggttctta tagagagagt ttacaaaaac actaatatag
     7921 aatttgcaaa gagacaaaat gatgcaggaa tgcttggagc attatataat tttttaaaca
     7981 aaatataaag aatagaaggg tttttaatga gtattttaga acaattggaa tcaccaactt
     8041 ttaaagtcac aaaatcagat aaattattaa tagcttatat aaaagaaaat atagatgatg
     8101 tattttataa accaatatca caaatagcta aagagagcaa tattggtgaa gcaactataa
     8161 ctaggtttgt aaaaaaaatg aattttaatg gattacagga ttttaaagtt actttagcac
     8221 aagaaatatc aactatcaat aaaaaaaata ttataaataa aaatatacaa aatgatgagc
     8281 cagctttgga tacagcaaaa aaacttttaa gttcaaatgt aaccacttta gagaacacag
     8341 tagagataat caatagtaag gatgtacatg actgcgcaag gcttataata aatgcaaaga
     8401 aagtttactt cataggtatt ggatattctg gtataatagc acaggattca aactataaat
     8461 ttatgagaat aggtcttaat tgtgtaagtt ttgacagtag tcatacaatg ataatgatgt
     8521 cttccataat ggaagaagga gatttaataa ttgcaatttc acattctggt gaaacagaag
     8581 agattataaa aactgtaaag cttgcaagag ccaataatgc taaaattata tcaattactg
     8641 aaaataaaaa ttctgaatta aaggatattt cagatgtaca cttatcttat gtatcgggag
     8701 aaacagtact ggaaaccggt tcaatatctt ctaaattagc acaatttttc attattgatt
     8761 tagtatatac acaggtagta aaagaacttt ctaatgaagc tattgaaagg aaaattaaaa
     8821 ctacaaatgc tataaaattg tttaaaaatg aataaaataa gaaatttgta atgttttata
     8881 gagctctctt aaaaatttag gaatggagag atattacatg tctaaattaa aagattataa
     8941 tattgataaa gtaataattt ttaataattt aagtactaaa acgaagaatc aacttaaaga
     9001 aaaagttaaa ctaaaaagat tacaaaaaaa agaaatttta ttttatgaaa aagatagctt
     9061 agacagtgtt tatatacttt tagaaggaaa ggtatctatt tttaagataa gtgaaaatgg
     9121 agagagaaaa gtaattttca tattaaatag tggggaagtc ataaatgata ttacacttga
     9181 cgcttctaaa aactctactg ttggctgtga agcatttgaa aattcaattg tagcttatta
     9241 tagtattgaa gaatttttag aaattatgca aaatgatttt caacttacaa aggatattat
     9301 ttcttatatg gaaagaagaa taagacgttt atatagacag ttgaaaaatt ctatatccat
     9361 aaaagtagat aaaaaacttg cagcaaaatt atataggtta agtagagaat ttggagtttg
     9421 ctgtggagaa tggactttac taaatgtaaa tattacagtt acatatttag ctgatatgct
     9481 tggctgtaaa agggagactg tttccagaat gataaaggta ttgcaaaaag aagaacttat
     9541 taaaattgac aataaaggtt tttatgttaa agaaactgaa ttatcaaggt attttaaaaa
     9601 taattaatta tgattaagta agagtattat tagaatttta attaaataaa aatattatta
     9661 agagaatcta tttatagatt cttttttatt gtctaattta ttaattttta ttttacccta
     9721 ttgaattgta taaaaaaaca aataagattc ttttttattc aaaaaattga gtagatagta
     9781 gttttattgt acttaatgat tgtatttaag tgattagatt tgaaattgaa taatgtagat
     9841 aataaaaaaa tattaacaga acttaataaa ctccattaat atttttttta ttatttttga
     9901 aactgtgata attatcacgg aagcatagaa aataaagtgt taagatttaa acaagatagc
     9961 aagtaagtaa aactaaaata taagaaaggt gataaaaatg aaactaaaat tagattcatc
    10021 taagttcaat gaaggtctga attatctaaa aaaagactac aagatatttg caccagtttt
    10081 aatcccattt aaaggtactt tttcagatac tgatgttata agatacaagg aggtaaacgc
    10141 ttttgaagaa atggaatttg ctaagaaagc aaacttttca ccaaaagaag ttgtacttcc
    10201 cataaatcaa gtattgtttt attttactga aaaagagttt aaggaaagcg atttagacaa
    10261 caagaaaatt ttaattttct taagagcttg tgacataaat ggtataaaaa gacttgatga
    10321 aatatatcta aataatggtg aagaaaaaga ttatttttat aaaaatatca gagataaagt
    10381 aaagtttgca cttgtagggt gtaaggaaag ttttagaaac tgtttttgtg tgagcatgga
    10441 ttctaacaaa actgataatt attcaattgg attaaatata gaaggagata cactgtactt
    10501 agatataaag gatagtgaat ttgaagtatt taatggagaa gcaactgaat ttaatgttga
    10561 ttatgtaact gataatttaa tatctgtaga tgtgccagag aatattgatt caaatgaaat
    10621 gataggaaat tcaatatggg atgaatatga tacaagatgt ataggatgtg gtagatgtaa
    10681 ttttgtatgt ccaacatgtt catgttttac aatgcaggat attttctata aggaaaacga
    10741 aaatgtaggt gagagaagaa gggtttgggc ttcttgtcaa gtagatggat atacagatat
    10801 agcaggtgga aattctttta gaaaaaaaca aggtgaaaga atgagattta aaactatgca
    10861 taaaatacat gattttaaaa aaagattcgg atatcatatg tgtgttggtt gtggaagatg
    10921 tgatgatgcc tgtccgcaat acatatcatt ttcaaagtgt atagaaaaaa taaatgattt
    10981 agtaattagt aaggaggaag tataaatgaa cccatatata ccagtaagtg caaaaatact
    11041 agatatagtt aaacatactg ataaagaatg gacttttaga gtaaactgca atacaaaagg
    11101 tgtattgcct ggaaagtttt atgagatatc tatacctaaa tacggtgaaa gtccaatatc
    11161 agtatcagga tatggagagg attatattga ctttacaata agaaatgtag gaaaagtaac
    11221 tagcgagttg tttaattata aagaaggaga ttcttttttt ataagaggtc cttatggaaa
    11281 tggatttgat gtatctcttt atgaaggaaa agaaattgta gtggtggcag gaggaagtgc
    11341 attagcccct gtaagaggaa tagttgaata tttttacaat aataaagaaa aatgcgaaaa
    11401 gtttaaatta atagtaggat ttaaatcacc aaaagatatt ttatttgcag atgatttaaa
    11461 gagatggagt aaaaaacttg atattttagt tactgtagat ggagcagagg aaggatattg
    11521 tggtaatgta ggattggtaa caaaatacat accagaattg gatatgaaag atataaacaa
    11581 tacttctata gttgtagttg gacctccaat gatgatgaaa tttacagtag aagaattttt
    11641 aaaaagagat ttagatgaaa agaacatttg ggtttcttat gaaagaaaaa tgtgttgtgg
    11701 aataggtaaa tgtggacatt gtaaaatgga tgatacttat atatgtttag atggaccagt
    11761 atttgattat tcatatgcta aaaatcttat tgattagggg gcgaagtttg tgataagaga
    11821 tttaaatacc aaaaaagtaa tgaaaaatgc ttatagaatt acaaaaacaa aatatgagac
    11881 ttctttaaga gttagagtac ctggtggtct tattgaccct gagagtttag ctgtagtttc
    11941 taaaattgca agtgagtacg gcaatggaca ggtgcacata actacaagac aggggtttga
    12001 aatattagga ataaatatgg aagatatgga agaaataaat aaaattatac aacctgttat
    12061 tgaaaaaatg aatataaatc aaagtgagaa aaacgcaggt tatccagcag caggtacaag
    12121 aaatatagca gcttgtatag gaaataaagt ctgtccaaag gctcaatata atacaactga
    12181 atttgctaaa agaatagaaa aagctatatt tccaaatgat ttacatttta aagtggtttt
    12241 aactggttgt ccaaatgatt gtataaaagc tagaatgaat gactttggaa ttattggtat
    12301 ggctttacca atatatgaaa aagatagatg tgtaaattgt ggagcatgtg taaaaaaatg
    12361 ttctaaaata tctgttggag cattaaaaac agaaaataat aaggtggtaa gagataaaga
    12421 taagtgtata ggatgcggag agtgtgtact aaattgccca acaaatgcat ggactagaga
    12481 tgagaaaaaa tactatagat tagctataat gggtagaaca ggaaagaaaa accctagact
    12541 agcagaagat tttcttttat gggtagatga ggacagcata ataaagataa tacttaatac
    12601 ttatagatat gtagaaaagt atatagataa agatgcacca ggaggaaaag aacatatagg
    12661 atatataatt gatagaacag gatttatgga atttaaaaaa tgggcacttg aaggtgtaga
    12721 gcttccagaa ataactaaaa tgtacgaaaa catatactgg agtggaatta aatatctata
    12781 aattgagtaa aacatatatt tgaataaatt aaaatttaat agaaggaaga gggaattatg
    12841 cataaagaaa ctttggataa attaactaat gcagctataa ataagataaa tttattaaac
    12901 acaagtaaag taaaatattt ggtatcttct gcgtttgcag gactttatgt aggtataggt
    12961 atacttttga tatttactat aggaggactt ttgactgatg caggttcacc tatgactaag
    13021 atagtaatgg ggttatcatt tgcaatagca ctaagtttgg ttataatgac gggaacagag
    13081 ttatttactg gaaacaatat ggttatgtct gctggtatgt taaataaagg tgtaagtata
    13141 aaagatactt ctaaaatatg ggcatacagt tgggtaggta atttaatcgg agctttggtt
    13201 ttaggtctta tatttgtagg aactggtcta gtagacaaag gacctgtagc tgaatttttt
    13261 gcaaatacag cagctagtaa agcatccatg ccatttacag ctcttttctt tagagggatt
    13321 ttatgtaata ttctagtatg tgtatcagtg ttgtgttcgt ttagaactaa tagtgatact
    13381 gcaaaaataa ttatgatatt cttatgttta tttgctttta taacatcagg ttttgagcat
    13441 agtgtagcaa atatgacaat ttatagtgtt tcattatttt caccaacaat aagtacagtt
    13501 actattggag gagctattta taatttagta gctgtaacac ttggaaatat tgttggagga
    13561 gctttattta tgggtcttgg aacttatata ttaggtaaag aaaagcttaa ttaaatttat
    13621 tgtataaatt ataactctct tgataaatac atttttagta ttattgagag agttattttt
    13681 atatttttga aaaaatattt gaaacaaaaa aaatatggga gcatacgaaa atatatcggt
    13741 tatatataaa aaaaatacgt ttatatatga attatatagt tctaaatata aaaaaagtta
    13801 aagtcctacc gtaaaaaagc gcattttgaa taagataaca aatatgataa aattggtctt
    13861 gttgaaattt ttagggggga tatgatgaca aagaaactag taatatcaaa aacaaagttg
    13921 tttattttta taatagcatt tatttctata tttatatatt tatttggaag taaaaataca
    13981 cttataggtg taggtatagt aacagctatg ctaacactct tggaaagaga tttaactatt
    14041 tctccaatta aaaatttact gaagtattta gcaataaata taatcttagg aatactatca
    14101 ttttttgcag tacaaaatat gtatttagga gttttattaa attttatagc attatttata
    14161 atagggtatg tatttagtta tgacttaaag agagcagtat atgtaccttt tggtctaatg
    14221 tatatattta tggtaagtat acctgtagga aaaagtgaat ttcctatgag attgtcggct
    14281 ttagctgttg gagcagttat aataatgatt gctcaatttg ttatgaatag aaataggatg
    14341 aaaaatgtcg gagataaaga actgatatct atatgtgatg aattattaga aaaaattagt
    14401 cttttaaaaa atactattaa tgatgatagt tctataatta aatctagtat gagaaaaata
    14461 gatagctgta attatagaat aaattctata tctaaaaatt tgaaaatggt tatttttgat
    14521 aatagaaaag atgactttta tatcagtatt agaggtatag acataatgaa tattttattt
    14581 tcattagaaa gaataagttt aatcctagaa agatacaaaa aggatagtaa agaatttgaa
    14641 gatgaagata taaaaaatat tttagaagaa agtagcataa atagtaaatc agatgtttta
    14701 gttgtagcaa cgaaagaaat aaactatatt aagatgtgtt tagaaaataa agataccata
    14761 actgataaag agatattagg atttagagat tatgttattg ctcaggatac taaaaatata
    14821 aatttaaaag aaatttatag tgttttagaa aatttatatg agtttttagt agattataaa
    14881 aaagtaaaat caagagacga aaagaaagct gaaagaaaat caaagatacc tcatgaattt
    14941 aaacgactat caatatataa gaaaaacttt aatttaaatt ctataagatt ttcttatgca
    15001 gtaaagattg ctttagctac agctgtagcg ggttttatta tggattattt ccatttaaga
    15061 gatggaagat ggataatgtt aactgtcttt tcattaacac aaccttacgc agaaaattgt
    15121 atacaaaggt ctagaaaaag aatagaaggt acatttatag gagctgtaat atttattgta
    15181 ttattttcaa taataaaaga tagtacatta aggtcgttaa tagttctttt agcagggtat
    15241 ataaactctt atgtagttga ttatagaaaa ttaatggtat gtgtaactgt ttcagctctt
    15301 ggttcagcag tagttatggg agacccaaat gttttaacaa taagcaggat ttcatatgta
    15361 gcattaggag caattattgc attaatagta aataagttta tattaccata tgatgctaaa
    15421 acaggctatc aacatgttat agaaatgtat aaaggaatag ttaaaaatat aattgatgaa
    15481 gtcaataagt ctatagaaaa tgtagctgat gtttactata taaaaaattt attattgata
    15541 ccatcactta ttgaagatag acttatgttg ataaatgcta tatataaaga taaacatcaa
    15601 gaggattttt tagaaaatca gaaattacta ataagtaata tgtataatct atatataaat
    15661 gttaataaaa ataaaataaa agatgaagat gttgaaaaaa tattgagaga tactaattat
    15721 atttcaaatt acaatgtaga taagtatgat gaaggaagaa gtgtaatttt agagagtatt
    15781 gtaaatacta aaagtctagg tgacaagata atatgtctaa acttactgca aactctaaat
    15841 ggtgtcaaag aaatgtatag aataagtaat ataactaaag tttcaataaa agaagcagct
    15901 tagatattaa attatagaat agccatatat ttaaaataaa gtatatggct atttgtttta
    15961 taaaatacta aaagaaaata atatataaaa aacaaaaaac atatatttat gaattttata
    16021 tgaaaatagt taaaatattg atttttttca ttggttgaaa aaacaattta cttaggataa
    16081 actataatag tattcaaata ttatgggggt tagatttatg agtaaagatg taaaggtaaa
    16141 gcgtaataag aaatatgata catttagtat aactatggaa cttgagagac ctttacttta
    16201 tgatgaggtt gagaacaaag atgataaaaa taataataga aaatttaaca taaagaataa
    16261 gatatttaag ggaataggta tactatttgt attgattttg cttggctttt tcatatggat
    16321 aaataaaaca tatgaacctc aaaagctggc aaaagaagct ctagtcagca atagtaaagt
    16381 tgaagtaact gtaaatgaaa acatttcatt tacaccaaaa ggaagaaaag tttctaaagg
    16441 tttaattttg tatccaggag ctaaagtaga aatagaatct tatgctccac ttgcaagaaa
    16501 aatagcagaa agtggatatg aggttgtaat cgtaaagatg ccacttaatc tagctatact
    16561 tggaactaat aaggcacaga aagttatgga ttcttacaac aacatagaac attgggtaat
    16621 aggaggtcat tcattaggag gagttgcagc atcaaatttt gcacgtgata ataaattaat
    16681 tgaaggcgtt gtatttttag cttcttatcc aatgggtgat gagttgaaag aattaggaaa
    16741 aaaagttata tctatatggg ggagcaaaga tggagtagtc aattttaaaa gtcttgtaga
    16801 atcaaaacaa aaacttcctg atgatacaac ttatgttgag attgaaggag gaaatcatgc
    16861 acaatttgga gactatggaa aacaaaaggg agataatgat gctattataa gtcaagaaaa
    16921 acaacttaat attactgcca atagtatcat taaattttta aagaatatat cttaaactaa
    16981 aatttaaaaa atataatttt ataagttatt atttaaagta aatattaata atataataga
    17041 atgaatattt tattacaagg gggattttaa tatggaatac gatatgccac tttacagacc
    17101 accaagtgag gcttatagtc ttataataca agtcacacta ggatgttctc ataacaaatg
    17161 tacattttgt agtatgtaca aatctaaaaa atttaaaata aagccactag aagtaataaa
    17221 aaatgaaatt gatatattta gaagatatta taagcatgta gaaagaatat tcttagcaga
    17281 tggagatgca ttgataatac caatggagaa attaagagat attattttat atataaaaga
    17341 agtttttcca gagtgtgaaa gaattactat gtacggctct cctaaatcta tagagaaaaa
    17401 atctgatgat gaacttaaag aattaagaaa acttggtgtt aaaatgattt atctagggct
    17461 tgaaagtgga agcaatgaag tattagaaga tattaagaaa ggttttagta gtgaacaatt
    17521 aataaaagtt ggtagaaaag ttaaaaaaag tgggattaaa ttatcagcca ctgtaatagc
    17581 aggtcttgga ggaactaaaa aaacacatca gcatgcagtt gatacaggaa aaatgttagg
    17641 agcaatttca ccagattatg tgggtgtact tagtttaatg gtagaaccta atactgaact
    17701 ttacgattta cttaaggcag gagagtttac aatactagag gataaggctg tgttacaaga
    17761 aattaaagag atgataaaga atatagatgc taatgaaaaa attgtattta gaagtaatca
    17821 tgcatcaaat tatgctaatt taaaaggtgt attacctgat gataaacaac gtctaattga
    17881 tgaaattgat tactatctaa gtaaccaaaa gctaaaaaga gaagaatata gaagactata
    17941 gtttttgata tatcaatcta tattgatagg taaatactga gattatttag atacaaaaaa
    18001 tcatgctaca ttaatagttg cttactattg atgtagcatg atttttttat taaaaaacaa
    18061 aatggtatat tcatatataa tatataaata ataatgaaaa tgtgaactag tttaatagaa
    18121 ttttacctat tataataaaa acaggaattt aaataataaa aagtaaagta actttttatt
    18181 aagtaacttt actttttatt ttatttaatg ttgtattaaa tttatatata ttaaaataat
    18241 atacaaattt tgaaaaagct cttataaaag gattattaaa gtaaagttta atataaataa
    18301 attataaaat attaaactaa tttatcaata tttcctgatt gaatatgctt aatgtttgat
    18361 tgaatttttt tatatggcca atcccaccat ttaatttcta ataattttga aattgtatca
    18421 ttattaaatc tcttttttat aatttttgca ggtactcctc caacaataga atacggtggg
    18481 acgtcattag taactactgc tcttgttcca ataattgcac catcaccaat atttacacct
    18541 gacataatta tagaatcata acctatccac acatcatttc caattataat gttaccctta
    18601 ttgtcccaag cgtccgttat atggttgata tcgagtttcc attcttcata aaagatggga
    18661 aaagtatatg tggattttga tttcattgag tgatttccac tagtcattag aaattttgca
    18721 ttacatgcta ttgagcaaaa cttaccaatt attaatttat cattgtttat agggtaatga
    18781 tacaatacat tgtttttctc aaaatctctt gggtcattat aaaagtcatt atatattgtg
    18841 taatctccaa cttctatatt ttcccttgtt attacatttt taagatatac agtttgataa
    18901 tcattacttc ttggatatac cttatttgaa cttggaatag tcatatttaa atctctcctt
    18961 taatatatat tatgtttttg actattccac tactacaata catctcttca cagaatcact
    19021 ccttgttaag aatcttcata agataatggc gtcctttttt agtgttaagt cggatattct
    19081 ttcttagtac attcttttta gcgtataact gacaaactgt cattttctta ccaatagtgt
    19141 caatgctatc atcaaggtca ttttgtattg ctttttcctt atcccttaat gatatatcat
    19201 cacgttttca ctttgggggt ttatcagtag gtacaagttt ccaagagtaa acaaattgtg
    19261 gtttaccaaa tgaatataca tacttatatg cgtatcttcc atcttttctc tggctctctc
    19321 cagttcttaa aattctatct ttattatctc gtcttttttc agttgacatg ataagaagct
    19381 cctctcaata accgaattga gcttgatacg tcatatgaat attataacat ataaggctca
    19441 atttcattag ataacgtcaa gtgtatcaac gattttctca aattgttttc tctttatttg
    19501 aatgcgattg ctattcataa tgacccagcc agaatcaagg ttttcatcag caagtttacg
    19561 taattttttc tctccaatac gaaagtattt tgacgcttct tcaatcgtca aggtatattt
    19621 ttcccaaata ggtacatcag tattgttcat agaatgtatc actcctttct atgttcgtag
    19681 ttcagaagta gatagatggc tttggtaagc tccattggta tttcaaccaa tcccattctt
    19741 atgggcagaa ccgtgtcata caggtgtatc attatactgt ggtacaggtt acggcaaaac
    19801 aaccgttcca aaggttattc acaaatcact atatagtagc tctcttgatt aaggtcatgg
    19861 cgtatagtat ttgttggcta tctgtatcac aaacgtatct atctacttta ttcagttgtc
    19921 aaagaacagt cggcattgat ttgcctataa aaaatcacaa tagcttgtgg gcttttatag
    19981 acaaataatt agtgattctc catgtaccaa atagcttgat acatggagag agggcgagat
    20041 taagacaaat taaatctcaa aatagcagtg attagttttg ttcctagcat tcgctttgtt
    20101 tcttcttcta catgaatata gggatttccg tattcatcaa ataaagtttt ctgtgaaaga
    20161 cgaatgatat agtgttcaaa atgtttaagt attccgttta ttgcatttac atcgccatgt
    20221 acagcaagta aaataattgt aaaaggtaat acttctttag taggtttcat ttcaatgttc
    20281 ctccatgagt tctttcaact cttttagtgc atttgttttg tgataatgta tggtattttc
    20341 tgctaaatcc atcaatgttg ctatgtcagc atttgtcatg ttgagaaaga atgaaagtag
    20401 aattatatct tgttttcttt ttgtaagttt tccgattgct tgtgccatta atacatcttc
    20461 aattcgtatg ttgtagtcgt aagaaaccat acagatgtag ttagaatcat aatcatctaa
    20521 aatactcaat ttacttaatt ctgcaagtga caaagattcg agagatacca aataatcatt
    20581 tctgcgttta ttttcgtcat aaatatctct agcatagtta cgcaaaactg ttttacagaa
    20641 actgtcgaat tgatgttcaa ttgtcttttc atcaaccata agatttcacc ctctttccat
    20701 cgcaaggagt taaataacct tgcttactcc tatcacagtg ggagggcgtt aaatcttcga
    20761 taaatttcaa aaaaaagaga aaaaaataca cactctgcct atagggcaaa aatgtgtatt
    20821 ctttaaaacg tatttaattt atattggttg taagccacca tcatattgat gtatggaaat
    20881 atgtattggc tagcttttca taaaaataca aagatgcata agaaattaat agaggggaaa
    20941 aacttattct gttattaaaa agtaggttaa ttctaataaa tgctattgcg gtcgaattaa
    21001 actatataca tggcaatcaa tcatgtttct attctttatt atgaattgtg acagtattct
    21061 tcaatcttaa aaccacattt ttccaatact ttacttgact ctctattgta ggaatataca
    21121 ttagcatgaa ttttgttaag gttgtatttt tcaaaggcaa ttttgcatat ttcttttact
    21181 gcatttgttg ctatcccttt gccccaatag ttcctagcaa gccagtaacc taattcagca
    21241 tttttattaa tataggtatc ttcttcaaat gttaggctga tacttccaat agccctttct
    21301 tgataaaaaa tagcatagct aagttgttta acatctggag ttgccaaaca aaaactaata
    21361 aaatctctag cgttttgctc cctataaggg tgggggaagt tgtcacgaag attctttgca
    21421 atttctttat cattcgcaaa ctcaactaaa tcggtaatat aagtataatt ccactctata
    21481 agttccattt ttcttctcct tttatgaaac agctacaaga aatctttcta agccaatacc
    21541 acttatcaaa cagcgttctt ttttatttcc aagcatttga taagtccagt caacaaatcc
    21601 accgtcaact atttcaacgg tttcatttcc ttgtttgact gataatttga aattgatacc
    21661 tttataataa ttattatcaa catcatcatt atgaattgat aactctatat taggaaaaat
    21721 aagtttgata gtttcaacca ttctgtcgaa aaatccgtca ttatctttgt atccgtttct
    21781 tttgcgtaga ataatagaga aacgaacatt ttctatttca cttaatatat ctttataata
    21841 gtttaagtgc ttttctaaca acattttttc acaagtgtaa gaccctgtat ctataccaga
    21901 tgaaaccata caatataagc caaaatgtga atataaacct ttacctacaa aattttgtgc
    21961 tcttattacc ctagtagtag tagccatgtg gataggtatg gtattatttc cttcttttct
    22021 ttttaattta tcagcaataa ttattgccaa catatttgag gggtctgcaa gtgtttctgt
    22081 ccctcgtaga gcacttacta cgttatattg atctacggag ccaaaaacgc tacagctacc
    22141 taatggggca gagggagaaa gcataatcgt cttgatatcc atattgaatg cttttctcaa
    22201 taattttgat tccaaagcat gaatttcttc tggatctata ccgctaggga tagtaaatct
    22261 atttagttgg tatgacttta gaatatcagt tgcagttaaa gtatttgctt gtctatcaaa
    22321 cacttctaat agaagtgagt tcaaatctga ttttgataga gctgataact tatcaactat
    22381 attcttatcc ccaattttat ttgtaattct acttaaaata tcactcatag ttatcctcca
    22441 tactgttact ttcaaaataa ttatagaatg gttacaacaa ctaatctagg tcatttgacc
    22501 aaaaatgaaa aaacactgaa tatttactat tcaatatttt tttactttgg gtttatttga
    22561 ttaaatcttg aaaatactgt aatgcaactc tttcagcttc aacctttcgg acaataggac
    22621 tttgtgataa ttcctctctt aactcaagat acaggttgat agtagcctgc aatgcattaa
    22681 cacagctttc atattcatgg ttagcaaccg tcttttctaa tgcttcaata tattggggtg
    22741 cgtccaattc aatttttcta actccacgag gtaatttacc taagagaagc tgtattaatg
    22801 gggcaagtac tgattgtctt aaaaaagata aaaaatcaat tgtttcaaat agttctctac
    22861 gtccaatctt agtgcaagcg taatgaaccc aaatccaaaa tctatcttca atccactgta
    22921 agtctggagt tgggaagtat gcttcttttt tagtataaca cctctcactt tcttttatta
    22981 gctatcaatc caacataatt atgcatagga gtattatata atgaagaaat aaaaaaaaat
    23041 aggtcacttg acccgaaata aaaaagcatt gaataattat tattcaatac ttttttgaaa
    23101 ttatacttat gttgtaggaa gattctttaa tacctctcgc tgattaatta cactaaatct
    23161 tttgttttca taagcaatta gatgttcttg tacacattca tttatagcac gattaatgct
    23221 tctaattgga gcgtttactt cgctagcaag ctgttttttg gtaagcagat ttaattcact
    23281 tctataatag tgtaaggata tacacctcag aagtcgttct ttaacactta aagatgataa
    23341 ttcttcaatt aattcagcat ttacaactaa tttactagat atttctcgaa tcagaaattt
    23401 tgtagcttca aaatctcccc ttaaccagtt taaaaaatca tctctatgaa gtttatatac
    23461 attacaatct gtgaaagcga caatttcaac gggttttttc ccatcattaa attgttccat
    23521 ttctccaaaa aaactaccac ttctataaag atgaatagtt gagatgtttc ctttcacatt
    23581 taagatgaat gcttctgcaa taccgtcaat cataagaatt acatattcat tttcttgctc
    23641 agcaaaaaga atagtctctt tctttttata cgttttttta acaagcttct ttttaatatc
    23701 ttcattcata aaatcaatta aatgcatatt catccctcct taccaaatct ttgattaaat
    23761 tatatcatct ttcaattagt taggtaagat aataaaaata tatttacata gaaactattc
    23821 cattattatt ttccagttgt catttttctg caaagtaagc tcaaactggg aaagctgttg
    23881 acaatagaaa tagtaggaat atacgagcga accatttcag tattcaactg ttgtaggttt
    23941 tcagtcagat aattcttcaa acgttcaatt cgtttgtcct gtgaatcttg cgatgctcta
    24001 accagtttca tagatagcgt tttaccttgt gccacctgca aaaaagacca tgcttcgtca
    24061 aggtcaacta tcttgaagat agagcggtca gtatgaataa agtctaatac aaaggtacta
    24121 ataacaatca acatagcaac actaagaagt tctattgttg tatattcttc aaaactagtg
    24181 tcggcatctg gcaatacgag gtcagcaact tgaataatgt tcagctgttt ctccaagctg
    24241 atagactgct tcacatttcc atcactaaac agtagatgtg caaaatcata gtctacaaaa
    24301 ctttcgatat ggtcggctat gctgttactg atagaagtat cttcttttcg taattcatca
    24361 ataacaagta ataacccccg ctgttcactt tgggttacat tgcggattgc ttttctaagc
    24421 actgggaact tatcactgtc acgactggag ataccagcaa ggaatgttag gatatcaatc
    24481 gctaaacttt cagaatcatt tttacggctc aaaatgacgt aagggtcaag caatcctttg
    24541 ttttcatcat tactggttaa attgacaatg tttatctcat gggctatttc tggtagcgtt
    24601 tctttccatt tgccgcgttc tgctttaggg tcaactatga ccgcctgtcc gccgaatagt
    24661 accgcatagt aaacaagcat attgttactg aataatttac caccacccaa tgaaccgata
    24721 aacgcagacg ctaacgcatt ggtaatagaa ccctttaccc cttgactagc aagggcaggt
    24781 tttagataga cattctttcc agtgtccaga ttgtagctaa gatagatatc ctcatgttcg
    24841 ccaagggttt gtgtcgctcc aaaaccaagt ccagccaaaa aatccgaagt gacatattga
    24901 atataatcat tgatgtaacg tttgctgaca ggaataaatt cgccatgcaa tcccaacata
    24961 tccccaaagg gacgcacgag cttgattgat aagtcgtcgt aaaagtcttt gacttcatca
    25021 caacgccttt ttagctcgtc aagatccttt gtagatacac ggacaacata gctaagttta
    25081 tacatagatt ctttacagtt aaaagtctgt ttttaaaaaa atgtataaat tataaatcaa
    25141 aatatagata ctatcaatga cccactaggt gcaaaaagca ttgatatggt taaattatca
    25201 gacgctaaaa gctgggctat ccgaatgagt gaagatgggt gtgcttataa atccattgac
    25261 aattataagc gctcactgaa agcgtcgttt ttcatggcaa ttcaagacga ctgtattaga
    25321 aagaatccat ttaattttca gttaagtgat gttttggaag atacaacaga accaaaaatc
    25381 gctctaacac cagaacagga aatgggctta ttatccttta tggagagcga taagacttat
    25441 tgtaaatatt atgatgatgt tgttatcctc ttagaaacag gactacgcat ttctgaatta
    25501 tgcggtttga ctttagaatt agatatgaaa aataaagcca ttatggtaga ccatcaattg
    25561 ttgagtgata ctaagattgg ctattatgta gaagttccaa agaccacaca gggtaagcga
    25621 gaaattccaa tgactgaaag agcttatcaa tcatttgaac gtatcgttga acggcgtaag
    25681 aacgctaaag cagaaccaat cattgttgat ggttaccaga acttcctatt tcttaaacaa
    25741 gatggattac caaaacaagc aactcattat caaagcatgt taagagcgtt agtcaagaag
    25801 tataacaaga cccatgagga caagctacca aagattacgc cacatacctt taggcataca
    25861 ctctgtacaa atatggctaa caaaggaatg agtactaata tattgcaata tattatgggg
    25921 cataaaaaca ttactatgac gcttggctat tatgcacatg gttcatttag ttctgcgaga
    25981 gctgaaatgg aacggttata atcctagtag tatgactaga gtttactact tatttactac
    26041 ttttgatggc attcttatac ggttaaatgc caacttatgc aaagtatctt ccaaaaagaa
    26101 aatgtcgata ctccttgtag atacaggata tatcgacatt taccaactta tgaaaagata
    26161 atcaaattta atataagttt ttcaaataaa aagatactaa attctttttc gagaatctat
    26221 ataacattac ttaagcttgg ttggggtata aacaattgag tagctacttt tgtatggtgt
    26281 tcaactttag tcaaaactct taagtaatgc aaatgattta gattcattaa tattcaatct
    26341 cttcataagt agtgaaattc gagttatttc tgcataatta tactatgatt tatagattaa
    26401 ttctattagc atccagaata atattgttta tatgaataaa atttaaagaa tattatgaaa
    26461 attataggat gttaaaataa atttaggtaa aaaagaagtg tttgtatatt atagagtacc
    26521 ttttctaaaa tatgtgggat tgttttaaga attagaataa aaattatttt taaattagta
    26581 tttttataat gtatagtatt tttaatgtat aattaaattg tgcagaaaat agaatttaaa
    26641 gtcctataaa atttctgatt atggaggaga atatgaaaag aaaagttcaa gtaaaaaata
    26701 ttactattgg agaaggtaga cctaaaattt gtgtaccaat tattgggaaa aataaaaaag
    26761 atattataaa agaggctaaa gagttaaaag atgcatgttt ggatattata gagtggcgag
    26821 ttgatttttt tgagaatgta gaaaatatta aagaagttaa ggaagttctc tatgaattaa
    26881 gaagttatat acatgatatc cctttattat tcacatttag aagtgtagta gaagggggag
    26941 aaaaattaat atcaagagat tattatacta ctttgaataa agaaatttct aatacaggtt
    27001 tagtagattt aatagatgta gagttattta tgggagatga ggtcatagat gaagtagtta
    27061 attttgctca taaaaaagaa gtgaaagtaa ttatatctaa tcatgatttt aataaaactc
    27121 caaaaaaaga agagattgta tctcgtttat gcagaatgca ggaattgggc gcagatttac
    27181 ccaaaatagc tgttatgcca caaaatgaaa aagatgtgtt agtgctactt gaagctacaa
    27241 atgaaatgtt taaaatttat gcagatagac caataataac aatgtcaatg tcaggaatgg
    27301 gtgttataag tagactgtgt ggtgaaatat ttggttctgc attgactttt ggagctgcaa
    27361 aaagtgtttc agcaccaggg caaatatcat ttaaagagtt aaattcagta cttaatctat
    27421 tacataagag tattaattaa attaattata tagaaatatt agatggtatt tttgaagata
    27481 taaattaaat ataaaaaact tttcagatag taaaatttaa attgggcaat tatatctgaa
    27541 aagcaactat ttagtgtgta ttttgaaagt gatatagaaa atcttataaa tcaactatgg
    27601 catatttaaa atatgccatt aaaacctact agaca
//
LOCUS       DAEMHZ010000045        27052 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000045 DAEMHZ010000000
VERSION     DAEMHZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.045"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..833)
                     /locus_tag="KPK72_001797"
     CDS             complement(<1..833)
                     /locus_tag="KPK72_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG2489017.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     E"
     gene            complement(1150..2478)
                     /locus_tag="KPK72_001798"
     CDS             complement(1150..2478)
                     /locus_tag="KPK72_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2489018.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWSKLN"
     gene            complement(2626..2955)
                     /locus_tag="KPK72_001799"
     CDS             complement(2626..2955)
                     /locus_tag="KPK72_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2489019.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKYHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(2979..4268)
                     /locus_tag="KPK72_001800"
     CDS             complement(2979..4268)
                     /locus_tag="KPK72_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2489020.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAEGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4284..4610)
                     /locus_tag="KPK72_001801"
     CDS             complement(4284..4610)
                     /locus_tag="KPK72_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2489021.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4745..5374)
                     /locus_tag="KPK72_001802"
     CDS             complement(4745..5374)
                     /locus_tag="KPK72_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489022.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5589..8246)
                     /locus_tag="KPK72_001803"
     CDS             complement(5589..8246)
                     /locus_tag="KPK72_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2489023.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTDAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8605..9549)
                     /locus_tag="KPK72_001804"
     CDS             complement(8605..9549)
                     /locus_tag="KPK72_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489024.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9542..10567)
                     /locus_tag="KPK72_001805"
     CDS             complement(9542..10567)
                     /locus_tag="KPK72_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489025.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10642..12210)
                     /locus_tag="KPK72_001806"
     CDS             complement(10642..12210)
                     /locus_tag="KPK72_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2489026.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTTNDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12323..13234)
                     /locus_tag="KPK72_001807"
     CDS             complement(12323..13234)
                     /locus_tag="KPK72_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489027.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13218..14204)
                     /locus_tag="KPK72_001808"
     CDS             complement(13218..14204)
                     /locus_tag="KPK72_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489028.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14564..14965
                     /locus_tag="KPK72_001809"
     CDS             14564..14965
                     /locus_tag="KPK72_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2489029.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            14968..15366
                     /locus_tag="KPK72_001810"
     CDS             14968..15366
                     /locus_tag="KPK72_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG2489030.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15717..15941)
                     /locus_tag="KPK72_001811"
     CDS             complement(15717..15941)
                     /locus_tag="KPK72_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG2489031.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(15976..17343)
                     /locus_tag="KPK72_001812"
     CDS             complement(15976..17343)
                     /locus_tag="KPK72_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2489032.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPI
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17427..18962)
                     /locus_tag="KPK72_001813"
     CDS             complement(17427..18962)
                     /locus_tag="KPK72_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489033.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTEITLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(18985..19419)
                     /locus_tag="KPK72_001814"
     CDS             complement(18985..19419)
                     /locus_tag="KPK72_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489034.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19680..20591)
                     /locus_tag="KPK72_001815"
     CDS             complement(19680..20591)
                     /locus_tag="KPK72_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG2489035.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            20887..21240
                     /locus_tag="KPK72_001816"
     CDS             20887..21240
                     /locus_tag="KPK72_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2489036.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(21459..22400)
                     /locus_tag="KPK72_001817"
     CDS             complement(21459..22400)
                     /locus_tag="KPK72_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG2489037.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(22501..23736)
                     /locus_tag="KPK72_001818"
     CDS             complement(22501..23736)
                     /locus_tag="KPK72_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG2489038.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(23780..24634)
                     /locus_tag="KPK72_001819"
     CDS             complement(23780..24634)
                     /locus_tag="KPK72_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489039.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(24762..25937)
                     /locus_tag="KPK72_001820"
     CDS             complement(24762..25937)
                     /locus_tag="KPK72_001820"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG2489040.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(26036..26920)
                     /locus_tag="KPK72_001821"
     CDS             complement(26036..26920)
                     /locus_tag="KPK72_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2489041.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         8639 a         4835 c         2775 g        10803 t
ORIGIN      
        1 tcttcttctt cagtaaacac attaaataca acttttaaat ttttttcagg attttctttt
       61 agccatagtc taactctact tacagcaaga ttagctgcct ctttttttgg ataaccaaat
      121 acacctgtag aaattccaca aaaaacaata ttttttattt catcaatttc ttttattgta
      181 tttaaacatg atttataaca atgaagcagt tgcttttctt gctctttact tggctgacca
      241 ccagatacaa taggtccaac agtatgtact acaaattttg caggtaaaca ataacctcta
      301 gttatcttag catctccagt atattctaaa tgcccttgtt tttttattat tttgtcacaa
      361 tcttctctaa gtctaggacc tgcgcatgaa tgtatctcat tatccacgca tagatgtaat
      421 ggttgtaaac accctaataa cttgttattt gcagcattta ctattgcatc tgctcttaaa
      481 ttcgttatat tacctctcca tatggcaatc ccttcctcta cttcttctat atcatttaca
      541 tctactaact ctctttcttc acattcaatc aacaataaat tatgtaacat attaagtatc
      601 tctggagcaa tttcttttgg tggatataca ttcaaaagtc ctctaagtat atctctaggc
      661 ttaatttcat catttgataa attgtatatt ttagaaggaa cttcttttga aaaatatgca
      721 attaaagtgt ttatattttt tcttctttgt tcgtcagtta gaggcttgat agttttatca
      781 aaatcctcaa ataaatttac atcatcagca taatctctcc acttcatagc catctctcca
      841 ttcttgtatt ttagacttaa aaataattat gtatttatgt acatatttat ttatttctat
      901 aaacacctac ataaatcctg ttaaaagtgt aaatttttta ttatgcaact tctctggtta
      961 tttctattga ttgatagtaa attattgtac ctaaatttaa tccaactaac aagtaactaa
     1021 acctactttc ctaactgcaa aatacactat gtttgccact tgtatctcat ttgtaaataa
     1081 ataaagtccc tacaaataca ttaactaggc tatgtactaa actgtaggaa ctttaaaaat
     1141 atattattct taattaagtt ttgaccaata atttttattt gcttctatta aatcatctag
     1201 taattctttt gcaactgttg cactaggaac tgtcttagat agcataagtg cttgccacaa
     1261 tttattataa gacttctcaa tccaagcatc aactactaat ttttcaactg ctaattgttg
     1321 ttctattaaa cctttttgga atgttggtat tttccctatt gataatggtt catacccgtc
     1381 ttttccaact atacatggaa tttcaaccat agcatcatct tgtatatttt ctatagctcc
     1441 attattttga actatcaata acatacgttc ctgtgtatta aaagctagag ctgtagctaa
     1501 atccactata aactctgcat gtattcctat cttaagccca ctgcccttag aatgtccttg
     1561 cttttctatc aatgcacatg aaccaaaaac ttctttttct cttccatcaa taacttcatt
     1621 ggcacgagta taatttacat cagaatgatt tacaacataa tctggaaata gatagtactt
     1681 taagtaacta cttggtattg tagttaaatc tgtttttact atttctttag taaataaatt
     1741 agtttcaaac caactatctt tcttgtcatt tttcattcct tcttcaccat ttataaaacc
     1801 atattctttt atatgttcta ctaattttgg taaaagttca tttccttttt tatctttaat
     1861 tgaagtatac caaccaaagt gatttaatcc aaagtatctt atatccatat gtttacgaga
     1921 tttgagtcct gcaatccttg ctatattatg ttccatacct attggcatat cacaaatatt
     1981 tattacccta gaattaggtc tcaaccttct aacagcttcc gctattattg atgctggatt
     2041 agaataattt aacatccaac aatttggaga atacttttcc atataatcta tgttttcaag
     2101 aacaccttct atagacctca ttccataagc tattccacct ggtccacaag tttcttgacc
     2161 aactacccca tgttttagag gtatcttttc atctagttct ctcatttcaa gttttccaac
     2221 tcttatatga gctaaacaaa aatctacatc tgtataagcc tctttagggc atgttgttgc
     2281 taaatactct atattagggt ccttttcctt tattagtatt tcacaagctt tagctacctt
     2341 attttgtctt tcttcatcat tatcatataa tttgattgct cttaatggta acctatctaa
     2401 gttatctagc aacattaata tgatttctgg ggtatatgta cttcctcctc cagctattac
     2461 tactgaaaat ttttccataa taatatctcc ttttctattt aaaaatttac tgtatttata
     2521 aatatattta taaatactag tactttaaag taaaagggag ttcatttatt aaatctatta
     2581 atttaataaa ctatagccaa acttcaagca ctatttacaa gaaatttaat tcttattaat
     2641 tcttgggtcc atcatctcaa atatatctac aagttcagat actaaagaat gagcaaataa
     2701 tgcatttgaa taatggtctt cagagtgcac aactaaaaaa tcagtcccac tatcttcact
     2761 attcatagca tttgatagta attctaaatg atactttgat gctgtttgta ttttctctga
     2821 ggctgaattt aaaaggtgtt ttgctttatt gtagtcacct ttttttattt ccttaagaga
     2881 catcattaca tcacttttag catctcctgc ttctgatatt atactaaata ctttttcttc
     2941 catttcaggt accatatcct tctccttcta atgacttgtt aaaaatcaaa gctatcatca
     3001 tctaatgttg tagctaattc tgcattttta tttaattctt ccttctccat tatcttaaag
     3061 aatggataat atatcaaggt cataagtatt atacttgcaa atactaatac gattgccttc
     3121 caatctaatg tcatcaaaaa tgctgcaacc cctggaggta aaaaacctgg tggttctgca
     3181 ataggtaatc ctactaatcc tacttttaat gccataaaat taagtgctcc aaccaataca
     3241 ggtccaaata caaatggtac aaacatatat gggttcataa ctattggtgt tccaaatatt
     3301 actggctcat ttattccaaa tagtgatggt aaaatcacaa cttttcccat actattaaaa
     3361 cttttacact tacttcttga cataaggtac actaatccca aagttatccc tgaaccagtt
     3421 acttgcaaga aaccatagta aaatcctatt gtaaacacat attttgggtc tccttctgct
     3481 gctatcagtg ttgcattttc tgccatatat gtagctgcta ttggcatcca tatacttgaa
     3541 gttattgctg ctccatgaag cccaaagaac cataataatt gagttataaa tagtattata
     3601 gttactgcaa aaacattatc tacactgctt atagctggtg ctaagaaatt cattataata
     3661 gctggtagtg gttgacctga gacattttta gttattgctg ctattgctgt aaataacatt
     3721 atacatacta tcattggagt taagtttcta aaacttttat ttactacact aggtacttct
     3781 tttggaaatc taattacgag atttttatca ttcataaact tagtaatatc aactactaaa
     3841 agtgctacta atatacttac aaataaacct tgtgcatcta aataacttgt atctaataca
     3901 ccatctacta cttgtccaca tacaacaagt tgtgcaacaa ctgctgtcat acctatttct
     3961 acatgattaa gattatattt tttagctagt gcctgagcta taaaaaatgc tgaatatata
     4021 gataataaac tcatagtaaa tttattaggg ataagtaatg tatccatatt ttcagataat
     4081 aatgtttttg cacttccttc aggtaacata ttgtttaatg ccataaataa catacttaaa
     4141 gaaccaacaa ttattattgg ggtaatactt atcatacctt cttttatagc ttgaagatgc
     4201 ctttgttttt caatcttttg tgctattgga agaagaaatc ttcttaaatt attttgaagt
     4261 gcttccattt ttattccccc caattatcta tttttatata attcgattgc cgacttcata
     4321 acagaaggag catccatacg accataatgc ttagtatcta ttaaaccaac tgctattttg
     4381 taattcttac aagcttcaac tacatcatct tccttaaacc taatttgtgg tgctacaagt
     4441 actacatctg tatcattagc ttcaatgtat gattttaaat tttcaaatga tgttgcctct
     4501 atatttatag ttttagtttt taacttttca tgctcactac aaaaactttt catcttttcc
     4561 acaagtacac ctgttgaagc tccatttgca catactaata aaatattcat aattaccctc
     4621 ctacaatatc taacctcttt tgttttctaa ttttttatca gccaattctt actctaattc
     4681 ctatcagttt ttactttaat tcttattatt ttattttaat tcttagtaat atttactcta
     4741 attcttagta ctgccaatga ttaaacttga aagccgtatc tataacttct tcttgcttat
     4801 atttttctaa tatttttaag tatctttctt tataatcatt atctacttct ttttctggta
     4861 ttactatact agcttcattt aaaaagtgga atgaatccct tcccatttct aatcctctat
     4921 atgtatgctt gtcaagtgcc acgtccataa cttctggaac tttacccatt gcaaatcctt
     4981 ttataattat attttttaat aaatcacttg atctatcttt ctcacatgca caaagatatc
     5041 ttattgcatg tatgaaaaat aaaggtctgt ctccatctgc atatgaatat tcctttctta
     5101 cttgatttaa attatttatg attattacag ctgatgggtc ccccattcct atatcttcaa
     5161 ctgatattgt aagtatcctt ctccatagct tatcttctaa ttctggtgat gatatatata
     5221 gttcataagc aaattcacaa gcttcttcca ccatatttct tcttatagct ttttgaaatg
     5281 acgatattat ttcgtctgca ctaaatccgt ttcttgtagt cattcttgcc catgggtcat
     5341 ttgccttttt atctcttaat attttatctt gcatattatt tactcctttt tatttaaatt
     5401 ttaattattt atattactca tttaataata taagcaagta ttatgccagt ttttttagac
     5461 actttaaaac actgttattt caatcatttt tatcatcaaa acttagatac tttgtctaag
     5521 tatatttata tgtgtctaat attaatatta ttttagacta aaaaaaatta ccaaataagt
     5581 ttttatactc attggtaatc tttttcgatt atttctgcat attctaatat taataatatt
     5641 tgatagcact ctttttcatt aatttctata ttatattttt tatctaattg agatactata
     5701 tcttttattt tgcttaggct cttgtgataa tcaagtgttt tttctttatc aagcttttca
     5761 atttctctag tatttccaat caacccatct accatgaaag ctaaatgcat cataagtcca
     5821 attactttat cgtatttctt ttctatatta aatactaaaa acaattcatc agacacatat
     5881 tttatatctt taaatatcct attataatta gagcttttta aggatgtctt taaaccttca
     5941 actacatcat cataacctat acttatcttt ataatatttt ttaatttatt gatattttca
     6001 tccttatcta tatctttttt gctaatatag tcaattcctc taatattaat tctttgttca
     6061 ttgattactg caacaatatt actactttct ttaagtttcg taatatttaa acttaaatct
     6121 ttttctgttt tatatgggac agatataata tctattccat ctaagtacct actcatcttt
     6181 tcttctaata tttttcttgt tttattatct acaccttctg aaacatgtga tgctataata
     6241 attgttttag acagattttc tttattttga atttcattag ttccaaaata gtactttctc
     6301 gtgttagtga tagagttgta tacgtcattt aaaggtaatc ccatattggc cttctgaact
     6361 gcttctaaca cagttaatgt cgtcaccata ggaattactc ttactccaca ctttttttct
     6421 ttctttataa tttcatcaaa agagtttaat gaacccatat ctgtaaatag taatacatct
     6481 ttattgtcta tattattaat taaattttta aaatctacaa ttacttcatc atagctttta
     6541 tctaacttca tatcgaaaga aatagcactg cactttccaa taatatcacc aactgctttt
     6601 accatactac ttgctgtagt ttctccatgc atagcaacta taatattaat gttattcact
     6661 attttatttt gttcatcttg tttagcttgt actatgaata atgtaatata tgctacagtt
     6721 tctatagata aaaataaatc aaattcattt tcaattattc caactatttt taatgctgtt
     6781 ttaaattcac ttctgttatt tcttctaatt tgattcaaat ctatgttgtt tgtagagcta
     6841 cttttatcca atacactttt tatataaatt gccattgact taaatattga ttcacttata
     6901 tctatttctt catcttcttt aataatttgt cttattgatt cacacatgct tttaaactta
     6961 acattattaa atagagaagc atctgctata ttcatttcta cagatttatt aagattagta
     7021 tttactgcat taataatatt tatatcattg tagttaataa cttcttcttc tcctaatgac
     7081 gtaaatacaa aatattctat tccatgtgtt ataacttcat ctgctttaat agacttctct
     7141 ctataatcat cactcacttc atttatagat ttatcaaaat attttttctc tatataaact
     7201 ggttctttat tatctctttt atatcttagg tatccatttg caacagctaa ttttatatca
     7261 ttttgaagtt gacctacatt atttttacaa tcataaagta atagtgattt aattatttct
     7321 ttatgtactt ttattgaaat agaaatactt tttgattctt ccttgaaaaa atttttgact
     7381 aattccattc tttcaatcat tgtcctatct ctcaatgatg gtatttctat tttcattggt
     7441 attcttctat tgaatgtatc taatagtgtt gatgtcggat tttctgtagt agcacatatt
     7501 attaatacct tagactctct ttctttcgtt gcatctccta gtcttttaaa tgttccctta
     7561 tctataaaag taaacaacat ttcttgacct tctgctggta gtctatgaat ttcatctaaa
     7621 aatagtatcc ctccatttgc aatttccaaa agaccttttc tggaagattc tgcatctgta
     7681 taagctcctt gttctacccc aaaaatatgt cccataagta attgtgcatt attagcataa
     7741 tcagcacaat taaatgttac aaaaggagct ttattttttc taattccact atctatagaa
     7801 taatcgtaca tatatctagc aatcatactc tttcctactc ctgtttctcc atagataata
     7861 gtatgaagtc catttgtagg atagagcatt gcgaccttgc atttttctat tataggtctt
     7921 aaactatgtt ctttacctat tagtgtttca aaactatctt tttttatttt gttttctact
     7981 tttgttcctt tttttatgcc aagcaactca ctttgtgtat caagcaattc attcttgtta
     8041 aggttaagat ttgcagggat ataatacaca ggtcttcctt ttatcttcgt aaccataccc
     8101 tcattgaata attggtttaa atatgtactt atattgtttc ttgcaattcc tgtcttttct
     8161 gataattggt tagctgtaat ccctttttca ttttccctat atgatgattc taaaactttc
     8221 attactttat cttttttata tatcattaca agctccccta cattatagta ttttattaac
     8281 taaaacccct atacacactt tattttttaa taaactctta ttctttaact aaggttataa
     8341 tttatcaaac tttccttact ttaaattata gaaaattatt gtagaaaaaa taagattatt
     8401 gctcattttt tctacattac tatttttaat tttatagtac tttcttaaca acatacttta
     8461 cttgttttta attttattat atttgaattt attatatttg aaaagtagaa ataaaaaacc
     8521 ctattttgga gtagttttaa aatttctcca aaaatagggt tttagtttgt atttcaaata
     8581 aatacctgtt caagtatttt tatactaaac aacttttaaa ttttgttggt atttatttat
     8641 gtctgattta gaagaatata caaaatgtcc atctgaaacc tcaatccaac ttaataactt
     8701 ttcatttgaa caatcatatc cattagaatt atactctatt ctatttcttg acttagcata
     8761 tctagggtca ggaacaggaa ttgcagaaag tagtgatttt gtatatggat gaataggatt
     8821 attatacaat tcatttgaat ctgccaattc tactaatttt ccattataca taactcctat
     8881 acggtcacta atatgtctca ccatagataa atcatgagct ataaataaac aagttaaatt
     8941 cttttcttgt tgcaattttt taaataaatt tactatttgt gcttgtatag acacatccaa
     9001 agaagctata ggctcatcag ctattataaa ttctgggtct actgaaagag ctcgtgctat
     9061 gccaatacgc tgtctttgtc cacctgaaaa atcagatgga tacttatttg cataacttct
     9121 atcaagtcct actagcttta gcatctcata tactttattc attctatctg ttttattttt
     9181 acagatacct tgaattttta gtggctctga aattatatct gcaattgtca tacgtggatt
     9241 taatgatgaa gtagaatctt gaaaaataat ctgcatactt ttgtttacat ctttttttat
     9301 aaatttgtac gtttttttat ctgatatgca atttcctttg taaattactt ctccaccagt
     9361 agattcatgt agtcttatta tagttttacc agttgtagat tttcctgaac ccgactctcc
     9421 taccaatcca aatatctcac ccttatatat atcaaaactt atatcatcaa ctgcttttac
     9481 agtagtactt ttgtccaaat gaaaatattg ctttaagttt tttatttcca gtattttttc
     9541 tctattcatt agaaatcacc cttccacaat ttactctaac tggtacatct acttcaggtg
     9601 catctgggtg caataaccaa gttgctgcac tatgtgtatc attaatttta aacattggtg
     9661 gctctttttc ataatctatc ttaagtgcat ttttattcct tattgcaaaa gcatctcctt
     9721 ttggtgggtt caataaattt ggaggcattc caggtatatt atataaatag tcatcctgcg
     9781 aatctaaagt aggtaatgaa cccaacaacc cccaagtata tggatgtcta gggtcataaa
     9841 atatatcctc tacagttcct atttctacaa tctttcctgc atacataact gctattctat
     9901 cagctatagt tgcaactact cctaaatcat gagttataaa tattattgac aaacctattt
     9961 tatgttgtaa atcctttatt aaatcaatta tttgagcttg tatagttaca tctaaagcag
    10021 ttgtaggttc atcagcaatc aatacatctg gattacaggc aagtgctata gctattacta
    10081 tacgttgtct cattccacct gaaaaatgat gtggaaattg tttaaatctt ttctcaggat
    10141 tatcaattcc aaccaattca ataagctcta tggctctctt ttttgcttca cttttactta
    10201 taccttgatg aatgattaca gcttctgcaa tttgctttcc tattgatata gttggattga
    10261 gagatgtcat agggtcttga aatatcattg atatattctt tcctcttata tcttccatct
    10321 ctttttcaga cttcttaacc aaatcacttg atttaagtaa aacctctcca tttttaatat
    10381 atccattgta tgggagtatc ctcatcaaac tcttacacag tacagatttc ccacaaccag
    10441 attctccaac tatagccaat gtctcacctt tttttagatt aaaagataca tttcttacag
    10501 cctgaacttc tccttcttct actttaaaac ttacagatag attattaacc tccaataaat
    10561 gttccaaaaa ccatcaaccc ctatgctatt caaaccttag ggatatattt ttatccctaa
    10621 ggtttctaat taatttcagt attattcaat tgtccagtca gctatgttcc agaatatacc
    10681 tacaccatga tgacctaaaa ctgtatcagg tgttaatcct tttatgtttg gttttccaac
    10741 atatattgca tctatataag caataaatgt ataaggcata tccttagtca tttctacttg
    10801 gaattgttta tataacttta atttttcatc tttatcttct gtttctcttg ctttttgaag
    10861 tattttatct atagtaggat tagagtaagc actgtaattt gcacctttat ctgttccaaa
    10921 tactttatat gtatggtcat ctgggtcaaa tggacttccc caacctatta aatgagcatc
    10981 ttgattagcc caatctgttt cagttacaac aacagcttta gcatcaacac caatttcttt
    11041 taactgttgt gcacatattt tagccatatc aactctaact tggtcactct cacctgctgt
    11101 tatctcgaaa gctaacttag taccttcttt ttcatagatt ccatcagaac ctaatttcca
    11161 acctagcttt tcaatttctt gtttagcttt ttctggatta tattcaaact tttctatatc
    11221 aggattatta tatggtccca tttgtaaagg tgaatatgct ggtacaccat gtcctaataa
    11281 tacactgtca actattgctt ttctatctat tgcatagctt aaagcatttg gtaatccttt
    11341 agctttttta tctttaaaga atttagaatt aaaattgtat aatatacctc tatagtcggc
    11401 tgttttcatt atatttactt taaaattctt ttcgtctttt tcaaagttag acatatcttt
    11461 aggagttatt tgagctaggt ctaattctcc tgattttaat tgcattgcct tagccttatc
    11521 atctggaaca atcttaaaga ctactttatc taatcctggt tcttttacaa aataatcact
    11581 attcttaaca agtgttatac tttgaccttt gtcccatttt tcaagtttaa atggtccagt
    11641 acctattggt ttttgattaa attcatcagt agctatatcc ttaccttcca aagcatgttt
    11701 tggtaatact cctactgtta aataatcaag cattgctgta tttggagctt ttaaagtaat
    11761 ttttatagta ttatcattaa ccacatcaat ttttgttata tcttcataat ttgatgctat
    11821 ctcagaagca ttatctggat tcattatagt ttctattgta aacttaacat catttgttgt
    11881 aaatttttct ccatcatgcc acttaacatc atctcttaaa ttaaaagtat atgtatttgt
    11941 tgcctcatca aatttccagt cctttgctag acaaggaacc accttattat tttcatcatg
    12001 agctgttaat ccattaaata ttaacgaatt tatttctcca tgttcatata gtgcaggatt
    12061 tatacttgta tagtcattac ttccataaac aagtaccttt ccatctttag gcgtatctgc
    12121 cttcttatct ttgtctcctc cactagaaca acctgccatt agactaagta tcattactaa
    12181 acttaaaact tttaacttct ttaatttcat gatgctcccc cttttgcttt ttataaaata
    12241 tttttcaaag ctttgacccc atttatttaa atagatattt caaatacctc agtatttgct
    12301 tatttaatac tcaaatgctg atttataaat tactagattt tttattatta ttctttctta
    12361 tataattacc tatatttgtt atacacacca atgtggtaac caaaaatata cccggaatca
    12421 gtataatcca ccatctattt gaaagtaatg cttcctctga caaagataac atacttcccc
    12481 aagaaacaat ttctattggt aaaccaattc ctaaaaagct aagtgtcgat tcagtaccaa
    12541 tagctgcgcc tatgtttgtt acaaccatga acattattga tgccacaaaa tttggaagta
    12601 aatgttgctt taatacatag aagaatcctc ctcccatgct cttagctgct agtatgtatt
    12661 cgctatttct tatttgtcta acttctgttc tcactatctt agatatattc atccaacttg
    12721 ttatccctat tactatggac atagatactg gatttgaatt tccaagtata gcttgtacaa
    12781 atattataat taatattgaa ggtatactaa gtatgatttc tgtaaacctc atcattgcat
    12841 catctacgag ttcagatgct gaaccactta tactaccata aacaattcct ataacagttg
    12901 atattacagt ggaaaatagt cctataaata aagatatttt tcctccatac catatcatag
    12961 aatatatatc tctacccatt gagtctgttc caaataaaaa agttttattt ggagctaaat
    13021 tagaacttgc taaatccatg tatgttggtt catgtgtcat tataaaagat gagaatacac
    13081 ttccaatgac tatgattgat aaaataataa ttgatatata tggtaattct ttaatttttt
    13141 tagataaggt tttctttgtt tctatatgtt catcttctgc tactaaaata taattttctc
    13201 ctacaatttc aaaatcacta tattgtggct tcattagtat cacctctatc atatttcatt
    13261 ctagggtcta ttttattgtt tataatttga gctaacatat tcccaaatac tactaaagcc
    13321 cctgttatta agcataaaac taataacata ttataatcat gatactttgc actttcaaaa
    13381 caaagtgttc ctagcccagg ataagaaaaa actttttcaa ctacataagt tcctcctagt
    13441 atatgaggta tcgaaatggc cattatacta atatatgatg gcattatatt tctcaggcaa
    13501 tgcttaaaaa caatagttct attatttaaa ccttttgcct tacataaaag cacataatct
    13561 tctcttattt cctctagtaa tttatttctt atcatatatg tgtaatacca tagatggctt
    13621 aatataagta ctgtaagagg taagattaga tgtgatatcc tacttaatat actggactct
    13681 tctcccattg aataagcacc actacttgga agtatactta agtttatact aaatataagt
    13741 atgagtacta aggcaaccca aaatgatgga atacagttgg ttattgttcc taatttgcat
    13801 atgattctat caatgagctt atcttcatgt aatgtacaaa atattcctaa caataaggct
    13861 agcacaaagg taagtatata tcctgaacca cctaatatta tagtgtttat atagacatca
    13921 tttataacag aagtaacatt ttgtttatat ttaaatgaaa ttccaaattc cccttttgat
    13981 gcatttgaaa tccattttat atattgactg tatattggtt catccaaacc aagttttttc
    14041 atagcatttt ctttttcttg tgtactcatt ctttctactc catccccata gtaagagatt
    14101 aatgggtctc ctggagcaag tcttgccatg taaaaaacaa taaaagataa taaaaacatt
    14161 actaaaataa actgcaacaa ctttttcagt atatccttta acaaacccca accccccttt
    14221 gttgcaataa aaaaaaccac ataaaagcaa acgtaaagta acgttaaaac cttcgtggtt
    14281 ccattatcat taaacaaaat taaattttct atatttatct atgagttaat tttagcatca
    14341 tttattaact gtgtaaacaa actttttaaa aattgttaaa atttcatcaa attattttat
    14401 ttatacctaa aataaaattt ttatattcta ttcttcatta ttttctataa aattataaaa
    14461 ttttaggttg actatataag aaaagtatat tatattagtt tgtatgcaaa ccatttaaga
    14521 ggtgatttta ttgaaaagta attatgactg ttttagaata gccatgctct taaaagaact
    14581 ttattctaaa actatgtaca cagtagaaga aaattttaaa gaaaatggat taacacacca
    14641 acagattata gttataaaac tgatagctca taatcaagaa cttacaatat cacaactatg
    14701 tgatgaaatg tctttagcta aaggtacggt ttcaggcata atcagcagat tagaacagat
    14761 tggatatata gaaaaattca agaaatctaa tgataaaaga aatacatacg ttaagtttac
    14821 aacaacagga tttgaatttg ccacaaattt taaaattaaa atgcaagaaa gttttgatga
    14881 tatttttaaa aattgtgatg aaaacgaatt gagtgactta gtaaaaaatc ttagaaatat
    14941 attggcaaaa gtaaaggaga gataattatg aattataaat taatacttgc aatagtattt
    15001 ataacatcag ctttaatttt ctacactata ggtgtatttg gagaaagaaa ggcaaaaatt
    15061 ttaaaaaaga aacatgtaat tatattttgg cttggtttta tatttgatac tttaggaaca
    15121 tttacaatga gtaacatagc caacagccat acttttgaag taaaatctgc tttatcacaa
    15181 aaccttcata gtattacagg tcttcttgca attgtactta tgctttttca tgcaagttgg
    15241 gctacttttg tcctatacaa agatgatgag gaaaagaaaa aattctttca taagtttagt
    15301 atagttgtat ggactatatg gttagttcca tattttatag gtatgtttat tggtatggct
    15361 ggatagtaaa ataaaaagtg tgtaagaaat actgtaggta aatttgtata agacaaattt
    15421 atctacatcc ttctttcaca cttttacata tttatttata atactccatt atttctttca
    15481 tgtttcttaa tacctatata gaatatttaa ttatttctat agtattttat tattttctca
    15541 cactttttca tatatatatt gttttactat tttttcatat gtatatatga aatatttcat
    15601 tatttctata atgttttatt atctttctca tactttttca tatatattgc ttcactattt
    15661 tttttacttc tttattaaat acttatgtat aacatacatt taataaacgt taactattaa
    15721 acacctaata cttgagttac tcctggaact tcactcacca aaacattttc aataatagct
    15781 ttgattgtca tagtcgctcc tggacaccca ctacatgccc cttgtagtct aactaaaacc
    15841 acaccatttt catttacatc tataagctct acatctccac catctctttg taatactggt
    15901 tttatctttt cctcaagcac tttttcaact ttttctctca tagctattcc ccccagaaat
    15961 attttattac caaaattata ccaatggttt aaatttttgt ccatctaatg atttatcaaa
    16021 ttcaattttt gcttcatcaa taattttagt atccataaat acatcataag cactacaagc
    16081 cagtacttta gctgaattta tcatagcaga aaatcctata tttgaacctg ctgatgaaca
    16141 tgactgccat gtatgacttg caattccaat aggccaagct gccattgcaa attgagccac
    16201 tggtattaca taacttacat ctccaacatc agtagaacca gctagtcctt tatctttgtc
    16261 accgtcaact atttccttgt gtatatagct ttctacaata gatttatcat tttgtaatga
    16321 tgttgctaca cctaatcttt cttctctagt aagagtatca cacaattttt ttgcaaattc
    16381 ttcttcttca ggagtatttt tagggcaacc aacaaaaacc atattgtcat aaactaaatc
    16441 agtcaaacat tggtttggta tatagtcata tattccatct gtaaccttat actccatttc
    16501 agtttctgtc atcattgctg ctccttgagc tacttttatt aatctatcaa gaacgtgttc
    16561 tgcatccttt gcttttttac ctctaataaa gtaccaactt tcagcaaatc caggaactac
    16621 attaggtctt ccaccaccat tagttataac atagtgcatt cttatagagt caataacatg
    16681 ttctctcaaa taattagctc caacattcat aatttcaaca gcatcaagtg cgcttctacc
    16741 attatgagga gcttgagctg catgggctgt tatcccttta aatttaaatt ttacagataa
    16801 gtttgccaga cttccacctc tccaaggtgc atttatatca aaaggatgcc atgtaaatgc
    16861 acaatctaca tcatcaaaac agccatttat acacataact gtctttcctc caccttcttc
    16921 ttcagcagga caaccaaaat attttattgt tccattaaat ttattttctt ctattaattt
    16981 ttttatagaa acaactgctc ctacacctgc tacacccaac aggttatgcc cacatccatg
    17041 tccagctttt ccttctacca atggctttct ttctgcactc acacattgtg atagctctgg
    17101 taaagcatca tattctccaa gtatgccaat cacaggttta ccctctccaa aacttgctat
    17161 aaatccagta ggtaaatttt ctacttcttc aattttaaat ccttttgact ttaggtattc
    17221 tttttgaatg ctacttgcat acttttcttc aaaggctaat tctggatttt cccatatttt
    17281 cttagataaa tcaattaatt cttctcttat ttcttcaaca ttatttatga tatactcttt
    17341 cataattttc ctccaaatat atgtattttt gatatgcttt acagattata ttatatacta
    17401 acattctatt tattcacaat tttttgttaa ttaattttat tcatatgctc ttctatggag
    17461 tatatcttat cttcactttg cctcttcata aatttaatgt actctatctt ccataaagta
    17521 atttcagttt tctcatttaa cataaattct aatatattaa tttctctcat tctaatatct
    17581 tcataaaata ctacactacc caatattgcc aattctaatt cacttaatga tgcaaacaac
    17641 attaaatact cttctccaaa taaacattca gattcaagaa tatcgataaa gtctttttta
    17701 gcattgatat tttcctttat tattcttatt ttttcaatta tagatgtact atctaatatc
    17761 tcattgaata tctctctaaa cgattcatca tccaatttac tttcatcatc aacaatataa
    17821 tcttctaagt gttcattttt atttttactc acagttacca tacttttaaa agtgttactt
    17881 tttattgagc ttagcacaga ttcaataaat atcttttcat attttttaat atatcttatt
    17941 acatcttcat cttctatgct taatcttttt attaatactt caattgcttc cataagtagt
    18001 aaagttattt ttttatttat tctaatttct ctgttaattt cttcaaaatc attataatcc
    18061 aaattactat ctacttcagt gttaaaacta tcatctactt caatcaattc aattatatta
    18121 ctttcttcag agtctataac tttatcttca gtaatattat tgtacatatt tttaaattgt
    18181 ttttctaaaa attcatactc atattttgaa atttctaaat tagtcacttc atttcctaaa
    18241 atagtagaaa aaatacaatt atttataata agttcaaata aatttacaag tataacatca
    18301 caatctattt tataagtcat cccatagtca taaaataatt tttctctatc ttttttctta
    18361 aataggttac aaaatctatt ttctatatct atactctcaa tataattctt cacataataa
    18421 atacccttaa catccatatc atctagtgcc aatggataat ctatacttac tggaacgtcc
    18481 tgagcttcaa attttacaat ataactttta aagaaacttg ataactccat caaggtatca
    18541 ttatatgcta ttgaattagt atccaatttt ctactcatca ttagtttata aaaatctttt
    18601 agcctaacta actcttcctc taatatttca attcctccaa ggtacacttt tttaacattc
    18661 ttagcagtca actccacaac tcttttttct atatctccaa gcttatttat atatgcatca
    18721 atagcatacc atattgaaat caacagtttt tcggcaactt ctgtttttac tgatgtgctt
    18781 tgaccatttg tatatttttt aatcaaatcc ataagtatta gagatatttc ttgttgtata
    18841 ttatatactt ctctttcatc tatcacttta caatttagac attccttaag tagagatatg
    18901 gaatattggt tctttattaa attattttcg tttatattaa tattacttaa attatttaac
    18961 atacaaaatc tctcctatca tatattaaac ttctccaaat ttaaatttct ttgcaaattc
    19021 gtctgcttct cctcttacat tgtccatatt gcctgaaaat ttttcataaa attcacccag
    19081 tatttctata atctcatcat cacatatctt gtcatcagtt tcatttttta aataataaaa
    19141 aatttcttgc atatcattaa tagcctctaa ataattgtcc acatttgtat attgtgatgt
    19201 atatatattt tctattaact gttttgtaac ttttatatct aattctattc ttccataccc
    19261 ctttaaagta ttatctctcg aatttattat atcttttaca tcagattcag aaagagctag
    19321 gccataatct ccagattcta tattagtttc aagcagttca tttctttcta tttcctttaa
    19381 attctgattt atagaactca aaataatagg tgatatcatt tttaaattct ccttttttat
    19441 tttttctatt gacttttttt gttttttatg ctatatttaa attagtatat attattttac
    19501 caaccactta taatagtagt gttaaaagtg gtttttgtca actcaaaatt actatctcaa
    19561 ttaaactttt caattttctc aacaaaaaaa ataccatcat ttagatatat aattttctat
    19621 aacaactttt aattcagtag ttatttacta aaatctaaac aatgatatat ttattaattt
    19681 tatttatctt ttttggctct acttctactt ttctttacat ttgagcaatt tctacaagaa
    19741 ggtgaacagt attgagattt agaatttgta ggaatataaa ttttaccaca gtacttgcat
    19801 ttatttaaag aagaaggttc acttgtaatt gcaaaggcat agatagtctc aatagctgtt
    19861 ttcaaagaat caaactccca tgcaataatt ggtttatcta attggcttat agaaaatcct
    19921 atcttttgag gattaaaccc tccagacata atagtaacac tttcagttaa tagattagat
    19981 gtatttttat atacacaaat ttgattaaaa tgcttacaca tatctttagc aaaacttaca
    20041 atccaatcta gttgttcagt gtagaacttt gaaaatacta aatctaaaat caatggtctc
    20101 tttccaaaaa actttggaga ttcttctctc tttgatacat ctatacccct tctatacttt
    20161 ttaaaataca catcttcatc acttgcaaat ggtataaaca tgtttacata ttcatctgta
    20221 ctcaatatat tctcttttgt aatataattc ttttcaatca tcagtactct ttcttctcca
    20281 actatatctc tattatatac acttgaatat attaaaccca ataatccata cttttttaca
    20341 aattcaagta cttctttctt tagtttcatt tcttttgtct cttttgatat atcttgtaat
    20401 gcctcttgtc ctatgcgaat tatatctact attatatcat ctgctacatc aaatgggtta
    20461 tacatggtaa acacagcatc ttctgttgga actatatata attcatcttc ttcagtaatt
    20521 ttatattcat aatcactata cctaatccaa tcacttgcaa aattccctat taattctatt
    20581 tttttattca taataatcat tcacaccttt caatgtaata tataatttca tattactatt
    20641 acacttttag tttattccat attttctaca ctgtcaacta ttctttttat atttttagca
    20701 tattaaaatt tataatagcc tttataatgg atttctacag ctaaatttct tgtttattga
    20761 tgactaattc ataattaaat ttataaaaac tatttacata taaaattaat ctctttataa
    20821 ttgatatgta atatattttt acacttacat attacatatc aattataaag agaggtatct
    20881 aaaatcatgg atagaaaacg cgaattaaaa caattataca aagaaatgaa atttgacaca
    20941 ggtgtattca taataaaaaa tgatattact aaaaagattt accttggaaa aagtaatgac
    21001 ataaaaagca aatttaactc tctcaaattt cagcttggtg ctggctcatg tatgataaaa
    21061 gaattaaata atgaatggaa aaaatatggt gaaaaagcat ttacatttga agttttagaa
    21121 cttataaaac atgatgataa caaaacagag aaagactatt tagaagaact agatatttta
    21181 gagatggttt ggttagaaaa attaaaggaa gataatactt atgaaattta ttccatataa
    21241 aaaataatat aatataagaa aagtagatag tgtctcaaaa tagacattta gttcataaga
    21301 tactttttta tataaaataa agtatctttt cataatttta acaatgaaaa agaggcttat
    21361 ctccatttga tagcctcttc ccatatattt tataccatta taaaatgaac aatctactaa
    21421 ttttcaatgt atatattcat tttactaata acactccatt acttctttac agcatccaaa
    21481 cattgctcta taatactctt atcacttata cctattgctg ttactttact agctccactt
    21541 aatatactct tatcactttt atttgaaact aaaacaacag gagcattttt tgcaagtggt
    21601 gatgcaacta aagcataaac taaatcatct ccacttgtta tatagaactc ttttgctcca
    21661 ttatagaact tttcaataac atttttatta gtttgatatc tatcaacacc accaattcta
    21721 gttgctctat atctgctcac tatgtaatca cttaatgtag actctcctcc aatagcatat
    21781 actggaacag gagaaactcc aaaccatggg tctaagtctg tgtcttcttc tgctgaaggt
    21841 atatcttttg tcaatattat aggagctcca tctctataag caacagatgc aacacttact
    21901 gcatcagctt catccttaaa tccatttact aaaaacatct tatcactgtt tttaattttt
    21961 tctatttcac ctgcaatttt ataacttgta tctactctat ctacaccatc aattcttata
    22021 acttgcattc ttctcataag taacattttt tctacatctt tacttataga attttctcca
    22081 cctattacat atatcttagt aactctattc agtctttgaa gtgttgcttc tggtatatca
    22141 tccttattag ttaataaaat agctgcatta tttactcctg caagaccact tgcagcaatc
    22201 ccatctacca ttgtagagtc tgcattgata agaactgctg ttgtaaaatc ttgcttatct
    22261 gaaataagcc cagctgtttc atatttatct actccttgaa tattttcaat cttatctagt
    22321 gcgtttattg atgttggaaa actaacactt aacagtaatg ctgaaatacc taacgccaat
    22381 acctttttac taatcctcat aaattacctc cctaaaacaa atatatttag tattagtata
    22441 atataaaatc atttaaaaat agtgtaaaaa tagttacact tttttataat ttaaataatt
    22501 ttagactaaa cacaattcat ccattaaaat aacttcattt attctatatg ctaagccatc
    22561 ccacgttttt tttgacatat ctacagatgt tttcttattt gagttattca ataaagattg
    22621 tatcttagaa tctattgcat tatacaaatt attttcaaaa tcatataact cttcatcttt
    22681 tggaatacct tctttctcca taaaaggtag agaaacatat tctatctttc cagaattatt
    22741 aatctcacta ccaatccact ctttaacacc aagaatatca gtagtgatta catctgtgcc
    22801 acaagataaa gcttctaaaa ctacaactgg taatccctca taaaatgatg gtaacacaaa
    22861 tatttgtgac ctgttgaata gctcagctaa atctctctgt tctaactttc ctaggaaatt
    22921 cattttaaat ggagacttac ttgccagttt aactatctca ttatatgatt cgatatcgct
    22981 tccagtacct gcaaaattga cttctattaa atctttacta tattttaatt tagatatagc
    23041 ttttataaat ggaatcagtc cctttgattt acatatctta cctgcaaata ctatttttat
    23101 tttatcatcc tttattttat aatttttatt ataaaatata tcatcattat aaccactacc
    23161 aattacaact actttacttt cacttactcc aaaagtcttt ataatatcat atttctgatt
    23221 ttcatgaaga gcaaatatta aatctaattt ccttatattt gctattatgt attctttctc
    23281 taaatcaatg gtatttaact gtcttaaaca agttccatga caaatagaca taacctttat
    23341 atcttttacc atttccctaa caaatgcagt taaaaggtac aaatgatgac atattataag
    23401 ttctggttta aaatcattca tagccttatc tatatttact ttaaattgat atttcaatct
    23461 atttatcatg tctatattta aatctttata tcttgtactt tcatatggca tagaatcact
    23521 catacctaca acaggaaaat ttaactcccc acaattatac tttactggat aaaaacttac
    23581 ttcacttgga aaacaattta catcatcatt tacatctata cccgcaatta cagcttgctt
    23641 atgaccgatt ttttcgaacc cttttatcat accagacaaa tatataccac tacctgtact
    23701 atttggtttt tgtgttatta tatgaagaat tttcactttt cacttgcctc ctttctaaaa
    23761 ttaaatagtt ttatacactc taatcattat atatatctaa atataaacaa gatgaatcat
    23821 cttttacttt aaatcttggc actttaaccg ctttatagtc attatcttca aaatccctta
    23881 ctttattatg tatatatcct ataccaaatt tttctgctat acgtatcaat tcttcttctt
    23941 ttattagatt atatctatca catgcacttg taaaaccatc actcgttagc atcaattgaa
    24001 aatctttttt tatttcaatg tatccatata tagaattttc cacagcttcc tcatcaaaat
    24061 ccaatatcca atagccctct tttgtatttt tcttcaatct attgcttact attgtatcca
    24121 taacactagt tctaatctca tcaaatgcca tctcttccaa gttagaaagt ttactcattt
    24181 catcaaatac agtattatca aatttacaaa tactcctatc ttttataact ttggaatctc
    24241 catttttaat aaacaaagca caatctccta ataaaaaata ctctacttta ttttcatgaa
    24301 attttacaac aactatagaa ctagatggta aatctaattt ttcaacctta aacccattta
    24361 atcttagttc atactctttt tttacttcta ttattccagt attaataata ctctttaatg
    24421 attcttcttt atttatattt ttatataaat acctattcca ccactgaaca taccaccttg
    24481 cgtcactttc agttgacact aaattttttc cattaagtcc tgtagcacca tctaaaaccc
    24541 atgccccaaa tggatttata ccgactacat cttcattaaa tttagctgtt ccttgattac
    24601 ataataggct atagtttctt atatttaaag ccatatcaac ctccaaattg aatatctaaa
    24661 aataaagttc tatttaaaat tatacagcaa ataaaaaaat ctacaacaaa acttgttgta
    24721 gatttttact taatctatat tatctttata ctttcagtat tttaatgatg gaataatcta
    24781 ttcttagtaa atgccatgac tatgttatac ttattacaag ttaatataac attatcatca
    24841 cgaatagaac cacctggttg tgctataaaa cttaccccac ttctttttgc acgttctata
    24901 ttatctccaa atgggaaaaa tgcatctgaa ccaagtgcta ctcctgttaa agttttaagc
    24961 catgctcttt ttccttctcc agttagaggc tctggttttt cttcaaaaaa gttttgccat
    25021 atgccatcag caagcacatc catataatca tcagaaagat atacatcaat agtgttatct
    25081 ctatctggac gacctatatc ctttttaaat tttaagttta atactttagg atgttgacga
    25141 agataccaag tatcagcttt gttacctgca agtctagtac aatgaatacg tgattgttga
    25201 cctgcaccta cccctattac ttgtccatct ttagcataac aaacagaatt tgattgtgta
    25261 tattttaatg taataagagc tataattaaa tcacgttttg cactatctgt aaaaatttta
    25321 ttatcagttg gaatatcttt taataggtct tcatttatta atatttcatt tctaccttgt
    25381 tcaaaagtaa ttccataaac atcctttgtt tcaataggtt caggagtata attttcatct
    25441 atttttacaa tattatagtt gcctttacgc tttcctttta atatttcaag ggcttcatct
    25501 gtatatcctg gagctattat accatcagaa acctctcttg ctataatagt tgcagtctcc
    25561 ttatcacata catctgaaag tgctataaaa tcgccataag aagacattct atctgctcct
    25621 cttgccattg cataagcaca agctaatgga gaaagctcta aatcatcaac aaaatatatt
    25681 tgctttaaag tatctgataa aggtacccct actgctgctc ctgctggact tacatgttta
    25741 aatgaagttg ctgctggaag acctgtagct tcttttaatt ccttcacaag ttgccaacta
    25801 ttaaatgcat ctaaaaaatt tatgtatcca ggttttccat ttaatacctc tataggaagt
    25861 tctccattct tcatataaat ctttgatggt ttttgatttg gattacagcc atactttaat
    25921 tctagttctc ttgccatata ctcatccctc tctataataa attaatcata agtttgatta
    25981 atcctatatt aattaaatta ttcgtactaa taaaattata tggcatattt atccattaat
    26041 caactatatt ttttaaaact cctattcctt cgactttaca ttcaactata tcgccatgct
    26101 ttaaatatac ttgaggattc atccccatag caacccctgc tggagttcca gttgaaatta
    26161 tatctcctgg ctttagagtc attcctcttg ataaatcatt tattatagta ggtatatcaa
    26221 atataaagta ctctgtatta gaatcttgtc ttacttctcc atttactaca ctacttatat
    26281 ctaattttaa aggatgttca aattcttctt tatgtactat acaaggtccc atagatgtat
    26341 gtgtgtctaa gctttttcct ctaaaccatt gaacatgttt atcttgaagt gctctttcac
    26401 ttatatcatt tactattgta tatccaaata tatattcata agcatcttct tttgatatat
    26461 ttataccttc tttacctatg attactgcaa gttctgattc atagtccaac ttctctacca
    26521 aatctccatg taagcttatc ttatcatcca ctccaattat tttgtctgct ctcttagaaa
    26581 aataaattgg ataatctggc ataactacgt tttttataac tccatctggg atttcattga
    26641 catggtcttt atagtttaga cctaaacata taatatcttt tttaggtctt acaattgggg
    26701 aacataattt aacttcttct aacttataac actttgcgtt ttcaaatgaa ttgtttttta
    26761 ttttttctaa atctcctgta gatacgtttt caataaaatc attcatatca gtatataatt
    26821 tacttaatcc taaactatta atttcatata tactatttgt ttctgaatta aatacaccta
    26881 ttttttcttc attgctacta caaaaagtca caaatttcat ttttattcct ccattcaata
    26941 agttgattaa tattatttaa tttttattag tttatataaa ttgtaattca taatatttat
    27001 atgtcaatac atatttatat attttaaaaa tattcaaata atttttatta aa
//
LOCUS       DAEMHZ010000046        26468 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000046 DAEMHZ010000000
VERSION     DAEMHZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.046"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..972
                     /locus_tag="KPK72_001822"
     CDS             136..972
                     /locus_tag="KPK72_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2489042.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1324..2901
                     /locus_tag="KPK72_001823"
     CDS             1324..2901
                     /locus_tag="KPK72_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2489043.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2894..4078
                     /locus_tag="KPK72_001824"
     CDS             2894..4078
                     /locus_tag="KPK72_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2489044.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRVKKTMDYIAKR
                     NL"
     gene            4096..4695
                     /locus_tag="KPK72_001825"
     CDS             4096..4695
                     /locus_tag="KPK72_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2489045.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4719..5189
                     /locus_tag="KPK72_001826"
     CDS             4719..5189
                     /locus_tag="KPK72_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2489046.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5548..6294
                     /locus_tag="KPK72_001827"
     CDS             5548..6294
                     /locus_tag="KPK72_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG2489047.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYGDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6355..7029
                     /locus_tag="KPK72_001828"
     CDS             6355..7029
                     /locus_tag="KPK72_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2489048.1"
                     /translation="MNDELKSILYNKGVDIVRFIDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7397..8743
                     /locus_tag="KPK72_001829"
     CDS             7397..8743
                     /locus_tag="KPK72_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2489049.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNSNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8770..10170
                     /locus_tag="KPK72_001830"
     CDS             8770..10170
                     /locus_tag="KPK72_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2489050.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10380..11816
                     /locus_tag="KPK72_001831"
     CDS             10380..11816
                     /locus_tag="KPK72_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2489051.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNTGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12160..13104
                     /locus_tag="KPK72_001832"
     CDS             12160..13104
                     /locus_tag="KPK72_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG2489052.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13273..14205
                     /locus_tag="KPK72_001833"
     CDS             13273..14205
                     /locus_tag="KPK72_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG2489053.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLLSSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14310..14777
                     /locus_tag="KPK72_001834"
     CDS             14310..14777
                     /locus_tag="KPK72_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG2489054.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15021..15875)
                     /locus_tag="KPK72_001835"
     CDS             complement(15021..15875)
                     /locus_tag="KPK72_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG2489055.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16041..18005
                     /locus_tag="KPK72_001836"
     CDS             16041..18005
                     /locus_tag="KPK72_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2489056.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADNLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSANPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18005..18466
                     /locus_tag="KPK72_001837"
     CDS             18005..18466
                     /locus_tag="KPK72_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2489057.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18469..18789
                     /locus_tag="KPK72_001838"
     CDS             18469..18789
                     /locus_tag="KPK72_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2489058.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18805..19905
                     /locus_tag="KPK72_001839"
     CDS             18805..19905
                     /locus_tag="KPK72_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2489059.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20025..22625
                     /locus_tag="KPK72_001840"
     CDS             20025..22625
                     /locus_tag="KPK72_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG2489060.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKSRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            23094..23429
                     /locus_tag="KPK72_001841"
     CDS             23094..23429
                     /locus_tag="KPK72_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489061.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            23566..24843
                     /locus_tag="KPK72_001842"
     CDS             23566..24843
                     /locus_tag="KPK72_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG2489062.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            24964..26336
                     /locus_tag="KPK72_001843"
                     /pseudo
     CDS             24964..26336
                     /locus_tag="KPK72_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT        10457 a         2695 c         4665 g         8651 t
ORIGIN      
        1 aaatattttg ttattgtagt attatagggt aaattgcaaa atatatacga aagtaaaatt
       61 ttaaatattg acattaaatt aaatatacta tactatgtaa gtgtgatgag agaatttttt
      121 tagaggggat ttatagtgaa tataaaaaag atatttaata acaatgtagt agtaagctcc
      181 ctagaagatg gcacagaaat aattgtcact ggtgctggag taggatttaa gaaaaaagta
      241 ggcgatttga tagatgaaaa tttgatttct aagaaatatt ttgttcaaga cgatcaaagg
      301 gacaaataca atcaaatact taataagacc tctattgaat actttaaaat ttcagaagaa
      361 attatagaaa aggctaatga agttttaaat actcaagtga atgattcaat aatattagcc
      421 ttaactagtc acatagaatt tgctgtccaa agagaaaaac aaggtataaa attaccaaac
      481 ttaatattga atgagacaaa acaactttat agggaagaat ttgaatttgg attatgggca
      541 attgatgaaa ttgaaaaaag gataggaata aaattgcctg aagatgaagc aggatatatt
      601 gctattcaca taatcaatgg tttggaaaat tctcaaaaag atgaaggtat taatatacta
      661 gagttttcaa aacaagttat tcagataatt gaagaagtac atggatttaa attggatgta
      721 aattcattaa attatactag acttataact cacttaaagt tttttgttca aaggattctc
      781 cgaaaagaaa cttacaatga tagtgatatt gaagatatgt ataagttagt gaataaaaat
      841 tacaataaac ctaaagtgtg tactgaagaa attgcagcat tggtattaga cagatttgaa
      901 tataagatta gtaaagaaga agaattatat ttaatgattc acataaataa aataacaaac
      961 agtggttgtt aggattgtaa ctgataaagc aggcaaaacc taagtcattg agcttaacta
     1021 ttagattaag ttttttgatt taggtttttt attagataca atcaaaatac agacagtgac
     1081 ttttaggatt gtaactggtt aagcaggcaa aacctaaatc attgaattta actattaggt
     1141 taagtttttt gatttaggtt tttttattag atacaatcaa atacagacag tgacttttag
     1201 gattgtaact ggttgagcag gcaaaaccta aatcattaaa cttaattatt aaattaagtt
     1261 ttttgattta ggtttttcta taaatttgat tatgttttta gataaaaata ggaggggaaa
     1321 attatgaaaa aaaataagtt aggtttatgg aacttcttcc aaatgttagg aaaaacgttt
     1381 atgttcccaa tagcactact tagtgtttct gggatgatgc taggacttgg ggcaggtttt
     1441 acagacccta agatgattga aatggttcca tttttaggga atgaatatgt taacttagtt
     1501 ttaaacttca tgttaacaat aggattattt gcatttaaca acttgggagc attatttgca
     1561 atggcaattc cactaggatt attaaaaaaa gaaaaagagt ttggtgcttt ttcaggatta
     1621 gtaggattta tagcaatgca cataggtaca aatttttatt taacaagagc tgatttgtta
     1681 gtaccagcag aacagatgtc aactaatggt caagctatga ttatgggtat acaaacatat
     1741 aatacaagtg ttttaggtgg tattgtagca ggattaattg tttatgcaat atatgataaa
     1801 gttagtaatt taaagatacc agaatcatta ggattctaca gtggaccaag attagttcca
     1861 attgttagtt taatagttat gagtatagtt ggattattaa tacctattgt ttggccacca
     1921 tttttcaatg cattccaaag tttaggaaga tggatttctt cagcaggacc attaggatat
     1981 ttttcatatg cagtggcaga aagagttaca attccatttg gactaaatca tcttgtaaca
     2041 tccgtattcc gttttacacc aataggtgga actgctgtta ttgatggaga aacgtacttt
     2101 ggtacattga acatgtttat ggcatatgta gaaaataatc agattatacc tcttgattta
     2161 gctggtaaaa tggaacaagg taaattgatg attcaatatg gattagctgg tgctgcattg
     2221 gctatctacc gtacagcaaa accagaaaat agaaaggcac ttaaaggact tttaatatca
     2281 ggagtgttga cagttattat aggtggtata agtgaaccaa tagagtttct attcttattc
     2341 atatcaccag cattatttgc tttccataca tttatgaatg gtctagcaaa catggtactt
     2401 ccatacttag gcgtgttgat gggattcaca ggagatttaa ttgcatttat aagttttggt
     2461 gtgttaagag gaacagctac aggatggcca atagcagtat tagtagcagt tttatatttt
     2521 gcaatatact attttgtatt taagtgggca attattaagt ttaatataaa gaccccagga
     2581 cgtgaggata aagaaataaa agtatctgaa ggaaatggtt cagatttcaa aaatttgtct
     2641 acatataaag gacaacaaat gatatctgct ttaggtggtc aatctaatat attatcacta
     2701 gataattgtg taactagatt gcgtttaaaa ctacaagatg tatcattaat aaatgaggat
     2761 gcaattaaag aggctggtgg tatagcagtt gttaaattag atgagcatac aatacaagta
     2821 attataggaa ctcaagtgta ttctttaaga aagcagatgg ataaagctat ggaaggtcat
     2881 ttggaggcat gtaatgatta attttgataa aaaagtagac cgattgggta catattgtac
     2941 ccagtgggac tatgtagaag atagatttgg taagaaggat ttgcttcctt ttacaatttc
     3001 agatatggat ttagagtttc catttgaaat aacagaggtg ttgcaggaaa ggttaaatca
     3061 tagagttttg ggatacagta gatggaaaca tgatgatttt agaaattcag ttacgaattg
     3121 gtatattaaa agatttaatt gtagcgttga tagtaactgg atatattata gtccaagtgt
     3181 aatgtattca attagcaaac ttattgagat gttaagtaat gaaggagatg gaatccttat
     3241 aaatactcct gcatacaatg ctttttatga tgtgataata aataacaatc gtaatattat
     3301 aaagtcacat cttgtaaata ggaatggatt ctattcaatt gattttaatg attttgagaa
     3361 aaagtgtaaa aaatctaaga tatttatttt atgtaatcct cacaatccaa ctggtagagt
     3421 atggagtgag ttagaacttg ttaagatgat aggaatttgt aagaataatg atgtcaagat
     3481 aatatcagat gatatacaca tggatattgt atatgacaat agaatgacac ctatcttaaa
     3541 tgttgcaaag gactatttag attctgtatt catttgtaca tcagcgtcta aatctttcaa
     3601 tataccagca ttgactgggt catatgtgat tataccaaat gaaggtatta gaaataaatt
     3661 tgaaaatatc actagatacc gtgattttgt aaattcgcca gcaattctag caatattagc
     3721 tacaatggtc tgttatgatg actgcgaata ttggttagat gaactattaa aataccttaa
     3781 agagaatctt aaatatacga ttgattatat aaaagataat ttagaaccat tgaaattggt
     3841 catgccagaa ggctgttact ttgcttggat agatttttcc gaacttaaaa taagtggtga
     3901 gaaattccaa aaattattga tggatattgg agaagttgct attatgtcgg ggtctgtata
     3961 tggtgaagaa ggtgagtttt atttaaggtt aaatgtggca tgttcaagag aaaaacttga
     4021 agatggttta aatagggtta aaaaaactat ggattatatt gcaaaaagaa atttatagta
     4081 ggaagggaga tagttatggg atatttaaaa acaatggttg atgttatgac tactgaaatg
     4141 aatgccataa gatgtttaat agatgaagca ggaatagaat atgaaagtat agttagtgaa
     4201 attgcaaatt gtgaaggcaa agtaattttt atgggtgtag gaaaatctgc acatataggt
     4261 aagaaattgg cagctacatt tgctagtact ggtacaccta gcttttttgt acatgcaaca
     4321 gaggcagtgc atggagattt aggaatgatt gaaagtaagg atattacaat tttaatatca
     4381 aatagtggaa attctatgga agtggtaaac tgtataaaat atattaaagc aataggttct
     4441 aagacaattg cattcacttc aaacagaaat tcagtactgg caaaagagtg tgattatgct
     4501 ttaatatatc ctgcaaagga tgaagcagac catttaaatc tagcaccaac aacatcatct
     4561 acaattactt tggttttagg agattctatt gcatgtgcat tatctaagag tagtaatttt
     4621 ggttcttcag atttttataa gtaccatcca ggaggaagtt taggtgagaa gttaaaaaca
     4681 gctaacaatg gataaaagca aaaaggagag tagtttttat gttcaaaatg tttaaaaaaa
     4741 aatcaataaa atcacccata tcaggaaaag taatagagtt atctaaaata caagatggtg
     4801 tattttctca aaaaattatg ggagagggtg tagctataga ttctacagga gacattgtat
     4861 atgctccaat caatggaaag atagtagtag tagcagagac taaacatgca tttgtaatag
     4921 aattagaaaa tggaatggaa cttcttattc atgttggttt agatactgta aatttaaaag
     4981 gcgaaggttt tgaggcattg gtgagtatgg gagaacaagt gaaggaagga acaccaatgt
     5041 tgaaaattaa ccgtcctttg attgaatcaa agggtatatc acttattact ccagttacaa
     5101 taactaatca ttcagagtac aatatgaata catgtaatgt aggcaatagt gttgaaggtg
     5161 ggaaagatac tgtaattgag tttaaataaa gaactaaaat acaaggttaa tatttataaa
     5221 tacctataaa cttatgtttt ataagattga ttgtatagat ttgttgtata aaaaagaact
     5281 ctttaattat aaattaagga gttctttttt attttgtaaa tattgtttag atttatatag
     5341 aaaaacactt agtaataaat agaataaata agtatttata tagctaaaaa tctaaatagt
     5401 tttgtttaaa tatattttta tattttgtaa tttcaaatta ataaataatt agatttgata
     5461 aacaaaaaat aaaaatgttg attatattaa taaactttaa tataatatat ttatgaaaaa
     5521 tgtaaactgt gaaatggagg tagtaacatg aaaattgaaa atctttctat tgatgatatt
     5581 aaacgaggct atatatttaa tgatgattta aattgttata gttgcattaa atgtacaaaa
     5641 acatatgagg taggagaaat atataaaata ggtaatagat tctttgaagc ttcaaaagct
     5701 attgaacttc atgtggaatt agaacatagt gattatttag agcaattgct taatagtgat
     5761 tcaaaataca acacattgac tgaaaatcaa aaacaattat ttcaattatt tattagtgat
     5821 ttaccagata aggagatagc aaaacaggtt ggagtatcca catctactat acgccatcaa
     5881 aaatttattt tcagagaaaa agcaaaacag gctaagctat atttagcact atatgaatct
     5941 gtatttgaag ataaatctaa taaaagcagt actataatac caatccatga acatgctcga
     6001 atggtagatg aaagatatgt agtaactgaa gaagagagaa aacacatttt ggatacatca
     6061 tttgaaagta tgaatccttt aaaattaaaa gtattttctt caaaagaaaa gaaaaaggtc
     6121 gtaattttat ctaagatttt ggaacaattt gaacgtggga agaaatattc agaaaaggaa
     6181 gttaatcaga ttttagaatc tatatatggt gattttgcaa caattagaag atatctaatt
     6241 gagtatggtt ttatgagtag aaataaagaa tgtacaaaat attggttaac ataattaaaa
     6301 taataaattt tactaatgtg taatgttatt tttatctagg ggaaggagtc aaatatgaat
     6361 gatgaattaa aatctatttt atacaataaa ggtgtagaca ttgttcgctt tattgatata
     6421 tctgaatttc caataaatca aacacaaggt ttttctaaag caattttatt ttgtataggg
     6481 ttatcaaaaa aatttattac agatatatac aataacttac ctacagatag tgatgagttt
     6541 ttagaaaaag aggagaaagc agaagaatta gctgactgga tttcagaata tattcaaaat
     6601 aaaggatata gagcgtattc tcaatcggag aaaaacaatc tagaacatgg atattttgaa
     6661 aaagcctata ttaatccaga aatgcaatca gggattagtc ctttaccaca taagacaata
     6721 gcaaatatta gtggcattgg atttatggga aaaaataatt tgtttgtaac agaggaatac
     6781 ggatgtgctt ttagcatgtg tacagtactt acagatgctc caatttctgt tgaaagatat
     6841 cctttgattg attcaaaatg tatggattgc aatgtttgtg tagagaactg tcctgcaaaa
     6901 gctatacatg gaaatgaatg gactttacct ggaaaaagag aatctattat tgatgtttca
     6961 aagtgttttt gtgtattgag gtgtatgatg agttgcccat ggtcattaag atatgcaaat
     7021 caaaaataac tttactagag aaggtattaa attgaatatt ataaaatttt taaaatgtaa
     7081 gattatataa gatttctcaa aataaaaatt tgtatagaac actcagaaat gagtgttttt
     7141 ctattgggat taaattatat tgatacaaag cttaactatt ttaatcatta tataaaattt
     7201 gtattaaaaa attaagaata acttggtaat agtgaaaata taatgagatt ataattgatt
     7261 tataatataa ctataatgat attgtaaaat gaaaattgtc atttcaaaat tcccaaaaag
     7321 agtaaaagtc acttcggtta tagtcaaaaa aatttttctg tgaaaagaat ttagatttaa
     7381 aagggaggaa ttaattatgg aaattgaaac taaaactccg gattggatta atgtactaga
     7441 attaaacagt aaaatagata ttactgggaa atatgatgta agtaatatga tacaagatat
     7501 tgtctctgat aagtcactag aaggttccat tatatatttt ccaaaaggta attatttatt
     7561 tgagaaaggt attaaaatta gtcaacaaat aacacttcaa ggagattctt attatggagg
     7621 agataatcaa gtatcaaatt tagataaaaa acaatcaatc ataggaacaa ccaattttat
     7681 aactcgagga gtttctaata tgagtattat tgcattgaca ggaacaagtc aatgtatcaa
     7741 aaatattaat ttttattatg atagtcatga tatagagaaa ataccgccta aaaatgtatc
     7801 tgctattaca gaatatggag aaacacaagg actatctcat tttgaacacc tgtttatttc
     7861 tggattttca gggataggga ttgaaattcc atactattca actggcaatg atataattgt
     7921 tagttcatgt ggtcttggta tgaggcttgg agagaagtca atgctttcta gtagcaagat
     7981 ttacgaatgt aaaaatggaa tggagattac tactggtgtt agcttaaata atgtgagaat
     8041 agaagaaata aaagaaatag gtattaataa taaaggattt ggttttaacc tgattatgaa
     8101 tcttatggtc gaccaatgtg gatattgtgg atttatgttt gaaaaaatgt gctatagcca
     8161 gataactgcc agaattacta gatgtggtca gtattataaa agtgttgatt ataatgctta
     8221 tgagaatatg gaagatagaa aagaaaaagc ttattccatt ttttatggag atattttaga
     8281 aagttgcaat attgtattga tgaatagcaa tattgatacc ttagaaagtg attcacttaa
     8341 tgaacataag atatatgtaa tcaaggcaaa tgaaacaaac aatgtaatgc taacatgtta
     8401 tgctgaagca gataacttta tacaatgtgc aaagggcaac ttgctgttag aaaatggtga
     8461 aaatacttat aaattttata atggtgagat ttgttctgta ggaggtgttc aaatttctga
     8521 tatagatgaa aaagatttaa tcaaaataat cgagaacact ctgtatatag atgataaagg
     8581 taagtattta atggttccta gattaaaaaa gaatactgta atatattcag tactagattc
     8641 tacagaagaa ataaataaat tacatggtgg aatatgggaa cagattgatg taaaagtatt
     8701 agctggggaa tccatgtact attataaaaa gataaaagat taaattatca aattaggagg
     8761 tagtgattaa tggaagagtc tataataatt aaagatataa gtggctggat taatgtcctt
     8821 gattatggaa tttgtaatga tgaaacaaaa gatgtaagtt ttaagttgca agagattatg
     8881 gatgctgtac cagaagattc tattatatat tttccaaaag gaaaatactt atttgaaagt
     8941 ggtgttaaga ttaagaaacg attaacttta tgtggagatt cttatatttt ggcgaatgtt
     9001 cctaaaaata ctgctggagt aacacaattt acttttaaag gaaagaaaaa tcctaaaagt
     9061 gaaaatatag caattataac agcaaaaagt gtaagacact gtattaagag catttgcttt
     9121 tattcagata gttgtgatag agatatgaca tctaatcatg aacctccaac agatggaaaa
     9181 cctagatttc atcataatgt aattataaat tatagaaata tatcggctat tgtatgtgaa
     9241 gataaacagg gagaatcagg tcattatgaa aatctatctt ttagtggttt ttctggaatt
     9301 gcacttgata tgcctaataa ttctacagca aatgatatta caatttttac atccggactt
     9361 ggaattagta caggagaaaa ttcaactatt gctaacagta aggtttgggg ctgtgttgat
     9421 ggaatgataa tttctactgg aacttttata aatggtatga gggtagaaga aataaaaaaa
     9481 gttggaatta aaaatattgg tagaggttta aattttatta caaatatcac aattgaccaa
     9541 tgtggatatt gtggagtttg gtttgattct atatccaatg ttcagatatc aggaaatatc
     9601 acaaggtgtg ggcagtacta ttatgatgta agctatgata attatttaat gttggataat
     9661 agagttaaag aagcatattc tttattctat ggtaatttat tggaatcaag taatatagtt
     9721 ttatcaaata ccaataaaga taattgggaa gatttcaaag aagataagca taaggtgtat
     9781 cttgtagaag cacttgaaac caagaatgta ctattaacat gcaatataga tgctactgac
     9841 tggattgtta agtcagaaaa aggaaattta acgtacaata atatgcgaca aactgttata
     9901 ttttacgatg gaaaactaag tagtatcaat ggaattggta tatctgatga agattatggg
     9961 gataaagtca agataaaaaa aggaaatata tatataaata gtaaggataa ggatattaag
    10021 atgcataaac cagaggtagg tgacattatg tcaacaatta ttaaagataa aagtattctt
    10081 tctgctcatt atgggggaac atgggagaaa attggtgaaa aaatagagtt tggaataact
    10141 gtacactatt ataaattgat agagaaataa gtaaatacgt agtatttgaa tagatgtatt
    10201 ttattggaga gctaggacta tagttttaat gtaaatggtt tggtaatagc atggattgaa
    10261 taatataaaa ataatataaa aaattaattt gattatgtaa aattctatga agtagtaaaa
    10321 ctgtgacttt agtcatagga ggttcaaact aagtaaaagg gaaaggaata tataatatta
    10381 tggaaggaag aactgggatt aaagatggaa gatggattaa tgtagtagaa agaggtatta
    10441 aaaatgatgg ttcgtacaac gtaggtcctg ctttgcagga tattattgat gaaatagaaa
    10501 cagatttttg tgtcttatat tttccaaaag gtaaatacct atttacaagt ggtgttaaaa
    10561 ttaataaaga aattgtattg caaggtgatt ctaattcatc tacaggcact acacaattta
    10621 taactcgtgg agtagaaaat atgagtatta ttacattgac agggaaaaaa caatgtatta
    10681 aaaatataaa tttttattct gatagttgtg agatacaggt caatgaagaa ccaccgacaa
    10741 aaggtaatcc taaatatcat tatgagatga ttattaataa gaatgaaaag aatgttgaga
    10801 tgaataatgt atcagcaatc ctgtatgata acagtaagaa tatgattaaa ggtctaggtc
    10861 attatgaaaa tatttatata agtggttttt cgggtacagg aattaggata ccatattact
    10921 caattgtaaa tgatattaca gtttcatctt gtgggctggg tattgataca ggaatagaca
    10981 caattatttc taatagtaga atatctaagt gtcaaaatgg aatgaggatt aggactggaa
    11041 ctagtataaa taatgtaagg attgaaaaaa tacagaaagt tggtcttata agtagtcaag
    11101 ttaaggggta tgaggggtat ggaagttata aaattaataa tattacgatt gaccaatgtg
    11161 ggtattgtgg cttttcattt gattcaatga aagatagtta tttttctggt attataagaa
    11221 ggtgtggtca gtattattat aatacagatt atgacacata tttaagtatt aaagatagag
    11281 tagaagaagc atattcttta ttttatggaa attattttac agaatgtaat attgatttag
    11341 ttagtgataa taaggataac tgggatgatg gcttagaata taaacataaa gtttatgtat
    11401 ttaaaaccct tcaaatacca aaattaacat tgagatgcaa tatagatgcc tatgattata
    11461 tcgttaaatc ggtacatgga ggaggtaatt taatgttaaa aaataacttt aacaattata
    11521 tatttattga ttcatctaat ccagaagtta ttaatggaat taattttaat tatcaaaaga
    11581 atggaaactt gttaaggtta aatactggta aattaagtta taaaaatgga aaggaagaaa
    11641 cagctatatt cagacctaat tatggtctta ttgtagcatc aacagattca gaatgtgaac
    11701 aaatagaggc tacctatgga ggaaaatggg agaagcttgg ttctgaagta attggagaag
    11761 aaacggtata ttattataaa catcatgaac cagataaaac aaaccaaata caataaaatt
    11821 tgtattatat aatgatataa agttttaata tatagtgttt acctttaatg taatattggt
    11881 atatttgtat agtattaaat ttgttaaaaa gtaaaattgt agggtgcttg aaaatagcac
    11941 cttttttagt ttcattataa aattagtttt gaaagtttag gaataatgta tatttatttt
    12001 taaagaatta taaccttatt aaggatattt taaatgctag aaatgaatca gtaaatcata
    12061 ctggaattat cttaaatgtt ttgattaaat taagtattta gagctataat tatattaaaa
    12121 atatattaga acataattat attactatgg aggttaaata tgatagtaag tgtgagtaga
    12181 agaactgata tacctgcttt ctatagtaag tggtttttta acagacttaa agaaggtttt
    12241 gtttatgtaa ttaatccaat gaatccaaaa caggttagta agatagagtt aaatccacat
    12301 actgttgatt gttttgtatt ttggacaaag gatgtaactc caatgttata tgatttaaat
    12361 aaattaaaag actataaata ttattttcac tatactataa catcatatgg aaaagaagta
    12421 gagacatgta ttttagataa aagaaaggta atagattcat ttaaggaatt gtctagaaaa
    12481 ataggcaaag aaagagtaat tttaagatac gacccgattt tcttaagtga aaagtacaca
    12541 gtagattatc atatcaaagc atttgcgagt ttatgtaatc agcttgatgg atttacagaa
    12601 aaatgtataa taagttttat cgatttgtat aagaaaacaa aatttaatac taaagcatta
    12661 catataaagc caatagaaat tacagaaata gaaacattat caaaagagat ttctaagatt
    12721 gctcataagc atgggataat acttgaaatg tgttctgaag aatataattt aagtaaattt
    12781 gggattggaa aaagtaagtg tattgatgat agattaatat caaaaattat aggatgtgaa
    12841 gtagaggtaa aaaaagatag tacacaaaga gatatttgtg gatgtgttaa aagtgtcgat
    12901 ataggacaat acaatacttg cagacattat tgtctgtatt gttatgctaa ctttaattat
    12961 gcaaaagtag aagaaaactg taggctttat aaggaagata ataaattact catagatact
    13021 ttaagagatg atgcaaaaat aagcattaga gatatgaaga ctattaaagt agataagaat
    13081 aaacaaatta gtatatttga ttaaaataaa tatactaatt accttaatat attaagaata
    13141 tagatacttt tgataacaaa attaaactta atagatattt tctatataag aaagatggtc
    13201 ataatatgtc aatttgacca tctttttgat ataattaaaa cacaaataat ttagtaacaa
    13261 aggaggcaga acttgaattt attaaaaaaa tatcctatac ctattgcaag tttgatttta
    13321 tcaattttta ctttaggtaa cttattacaa tcttatagtg aatcattacg taatgtaatt
    13381 ggatttatag gatttatttt gtatgcaatt tatatagtca aaataatatt acttagaaag
    13441 ggtgttaaag agcaattaga gaacccttta ttatcaagta catttccaac tataacaatg
    13501 gcaacaatga tatttgctac atatataaaa ccattatctt tagaattaag tattatgatt
    13561 tggagcatag gaattgtggg tcatcttgtg ttaatagtct tatttagtaa gaagttctta
    13621 gttaaattct caataaaaac ggtatttcca tcttggtata tagtatatgt tgggatagtt
    13681 gcagctagtg ttacggcacc agcatttaat caacttttga ttggcaaaat tgctttttgg
    13741 ataggatttg tatcttatat agttcttata ccagtaattt taaagagagt atggataatc
    13801 aaggaaatgc ctgaaccatc tttaccaagt atagctatat tagctgcacc agggtcacta
    13861 ttattgtctg gatatattaa ttctttttca gataaaaata atgtagtatt atacttgtta
    13921 ttcatattat caattgtatt ttatattata gttttgggat atttaccgaa attattaagg
    13981 ttgccttttt atccgagttt tgctgcattt acattcccta tggcaataag tgcaatgggt
    14041 atgaaactaa tgaatggtta tttaactaaa tcaggtcatt ttataagttg gatttcatat
    14101 gcttctaagt tagaagagat aattgctaca gtgattattt tatatgtttt agttttgttt
    14161 attatatatc ttagtaaatc tgaaactaca tctaaagcta aataagaatt attaaatatg
    14221 tgtatttaat aggaatattg atataaatat atgaagataa gataaaccat agaaacttaa
    14281 tttagcttaa ttttattgga ggaaaacaaa tgaaaacaaa agaagaatat ttaaaagaag
    14341 ttagaaacat atcagaagaa tactttagaa aaggagagtt tttttgctct gaagcagttc
    14401 tccagacaat aaatgatgct ttaggccaac ctttaagtcc agagataact aagttagcat
    14461 caggatttcc gataggactt ggaaaggcac aatgtctttg tggagcaata tctgggggag
    14521 agatggcttt aggtatagta tatggaagag ttcatggtga agctatgaat ccaaagatgt
    14581 ttgagcatgc taaaggtttg catgattata ttaaaaaaga gtatggtgca acatgttgta
    14641 gagctattac aaagaaatgg gatggtgata actttatgtc tcccgaaaga aaagagcact
    14701 gtattaaaat aacaggtcaa gttacagagt gggttgcaga taagcttata gaagatgaaa
    14761 aacttaatat caaatagtgt aatattagtt ggtgaattta taaagttaaa tttacttata
    14821 gtataagata cttgatattc tcgtattgat agaaaaattt atagcatatg tatttaatga
    14881 aatatactta tatttagagt gtggtttaat ttcataaata catatgctat tattttaagt
    14941 atataagcaa atttatttaa ataaatagaa acagttaatt tatatgtaca aaactatata
    15001 tgattagtgt aaataaatac ctattttata atactcaaca taaagacacc agacatgatt
    15061 agtattatac caataaaaat agcaagccat tcttttttgt tcttcttttc tttaaaaaat
    15121 ataatccctc ctaaagtggc tactatcaca caactctgag agatagaaaa acttattgta
    15181 actcccaatg tattagtcgt tatgaacata cctaaatttg ctattgacca caatatacca
    15241 gtaattaaat tgaataaaac attttcccta tatacaatct ctttagaaaa atacttgaca
    15301 ataatagatg aagttatcaa cataccaaca gattgaggta atataattct atatccttca
    15361 attaaaaata tttgattagt tacaacatat aatgataaaa atagagatga taataataca
    15421 actccaacac attttaacat atttttatct gtagattttt tagtttgata atttgtgaga
    15481 agtataccaa gtattataca taatattgat aaagaaccaa tcagtaacat tttagaactg
    15541 gtccattcat taaacaagat tacagctaat aatgtcgtaa aagttaattg tgaaccattt
    15601 gaaattggca ttactttaga tacactagca atttgaattg ctttaaactg cattaattgt
    15661 cccaaactcc agaatatgcc agaaatcata cttatacaaa atgaaaaaaa tgtaatttca
    15721 ggatgtataa ccagagttat tatagtggaa aatataaacg cacttatgga tgtacctaaa
    15781 agctgttcat gaggagaact tttctttaga tttgcaacta tgggcatcaa tccccatccc
    15841 attacaggaa gtaaagcaat aattattgtc agcattaaac tatttccttt catattaaat
    15901 ttagacagct tttaatttat catctaaaat taaagtgttc aatacataaa aattaccgta
    15961 tttaatcagg aaatattagt agttttttag aaaaacattt tccattaaaa taaagacaaa
    16021 gggaaggagt aaaattgaaa atgttaactg atatgattca aaatcgtgaa attaaaataa
    16081 taaagttttt attaaaagaa ggtcatacaa ctgtaaaagc aatagcagat aatttagatg
    16141 tttcagaaaa aacagtttca aaatctctta aagaagtaga agcagtgctt aaaaagatag
    16201 gtctttcttt aataagaaaa cctaagatag gtgtttatat tgatggagat ttaaaaaaaa
    16261 tacttcctta cctagataat aaaggaagtc aaattcctac aaccagagag gagagagtta
    16321 tctatatata ttcagtactt ttaaagagta atgattatat tactatacaa caactttctg
    16381 atgaactata tataagtcgt ggaactattg aaagggattt agcagaagtt gaaaaactgc
    16441 taaaaaacga agggatttcc atatataaga aacctagtaa gggaataaag ttaaatataa
    16501 gtgaaagaga taaaagacaa cttactgcta agtttataca taaattttgg agtagaaact
    16561 ggtatattaa gcaagagcaa gaaagctttt atcaagcatt tgaaaagata caggcagatg
    16621 taaatggtat tttttcggaa gatgatttat caataattat tgatattctt aggaatttca
    16681 gcagagaaag aaaatttgag ttctctgatt atgaatttca gtcattagtt attcatttgg
    16741 caatagctgt agagagaatt agaaaaggcg aatatataga agaaagtttg tatattggta
    16801 atatagaaaa taatcaaatg tcaaatacca attatttagc aaaacaatta gaagaaaaat
    16861 tttcaatatt attaccaaaa tctgaaattg gatatataaa tttacattta gtagcggctg
    16921 accaacatag agatactaat gaactaaaaa atacttcagc taatccttgt gagacaagta
    16981 caaataatat atcaaatctt cgggaaatta taaaaaatac attgtatgag acagcttatg
    17041 acaaggattt aattgatgga ttgacttcac atgtacattc atcaataaat agactgaaat
    17101 atggacttag tattaaaaat ccatatgtaa atacaatcaa acaaaacttt tctttatcat
    17161 ttgagcaagc tttagattta aagaaagtaa ttgaatttat atatgatatt aggataaatg
    17221 atgatgaatg tgcttatata gcattacatt ttgaagcttt tagagaaaga atgaaagaac
    17281 ttccacaaaa tattagagta attctagtat gcagtacagg attaggttca tctaggttat
    17341 tgtctgcaag aataaaaaaa tactttccaa tgataaagat tgaaaaagta ctttccgtac
    17401 aagaattagt atccagtaaa attgatgcag atttagtaat aagtactatt tatttagagt
    17461 tagaaacaat tccaaccata gtagttagtc ctattttaaa tgaaagtgat ataagaacct
    17521 tggaaagaaa tatacaaaat gtttcaagtt gtacttctaa gaagtataaa tctttcagaa
    17581 atttaatctt tgaagaaaat atattgttga atattgaagc ttcaaattat gaagatgcaa
    17641 ttaatcatat aacagataac ctaataaaaa aaggttttgc aaaagaaggt atagctaaaa
    17701 gtgcaattga gagagaaaat ttatcttaca cgtctttcca gtcaatagct acacctcatg
    17761 ctaatcctag ttatattaaa aaatctaata tatctgttgc aacgttaaaa aatcctatta
    17821 catggggaaa tgaaaaggtg gaagttattt tctttatttc attagaaaat gacaaaaacc
    17881 tagagcttga tgaaatatat gaagtttttt atgacttcat tgacaataaa aaaaatataa
    17941 atgacatatt aagtgcaact tccacaaagg aagtgtacga tttgctagtg gaggagagta
    18001 tatagtgaaa atatcagatg tattgaaaaa agagcaaatt gtttttaatc ttgatacaaa
    18061 tacaaaggaa gaaacgataa aaaaattaag tagtatattt gttgaaaatg ggattgtgga
    18121 caatgaagaa gaatatgtta agtctgtttt ggaaagagaa gaacattcaa ctacaggtat
    18181 tggtaatggt attgctattc ctcatggaaa aagtaatgct gtcaaaaaat cttctataat
    18241 atttgcaaag actaagcata agattgaatg ggatgcttta gatgaaaaac cagtagatag
    18301 tattttctta ttggctataa gcaatattga taaggatagc aatcatcttg tactattatc
    18361 taaattagca actaaactta tggatgatga caatgtagat gcactaaaat tagccaattc
    18421 agaacaagaa ataatcaata tatttagtga agaaggagag atgtagaaat gaaaagaaaa
    18481 attatagcag ttacagcatg tgcaacagga gttgcacata catatatggc agcacaagca
    18541 ttaaagaaag cttcaaaaaa gatgggtcac cttataaaag ttgaaacaca aggagctaca
    18601 ggaatcgata atgagttaac tacaaaggat gcacaaattg cagaagtagt aatatttgca
    18661 gtagatacta aagtcagaaa tgctgaacgt tttgagggaa aagaaatact agaagtacca
    18721 gttaatgcac ctataaagga tgcagagaaa gtaataaatg atgcattaaa gttaattgac
    18781 actaaataaa tggagggaaa tgtaatgttg aaaaagatag tctctgagtt aaaaaagcat
    18841 gtcttaactg gaatctcata tatgattcca ttagtaattg caggtgctat gataatggcg
    18901 atatctcgtg taggtggttc tttctataat ataccagata tatgggatgc taaatatgca
    18961 gaaagtgcta gtagtattgt tagattatta catgatttag atgggtttgg aggtactgca
    19021 ttaggattaa tgtttcctgt aattgctgca tttataggat tttctatagc tgataaatta
    19081 gctattgtac caggtcttgt tggaggtatg attgctaaag atataggtgc aggattttta
    19141 ggagctttgg cagttggtct tatagcagga tatacatgtt tgtttattaa aaatcatgtt
    19201 aaattaccaa aatcagcagc ttcaatagta ccaatattta tagtaccagt gtttgggaca
    19261 ttaatcactg ttgtattaat caattatgtg ataggtatac cttttgccac attaaatact
    19321 ggattagaaa attggttaaa tggattatct ggtacaaatc agatattaat ggcagctatt
    19381 ataggggcta tgatggcagt tgacttaggt ggaccagtta ataaagcagc tttaacaaca
    19441 tcacttgcat tattaacaag tggaatttat gcgcctaaca ctgctgcaat ggtaggaata
    19501 gttataccac cactaggatt gggacttgct actatactag caaaacaaaa atataataaa
    19561 cagttacgtg aagctggaaa atcatctctt ataatgggtt taattggagt aagtgaagga
    19621 gcaattcctt ttgcggtaga atctccatta aaagtaatac cttcatgtgt aattggaaca
    19681 gcaattgctt cagctatggc agttggttta ggttcagtaa atgcaactcc aattagtgga
    19741 ttttatggat ggtttacagt agagaattgg ccaatgtatg tgttatcaat agcagttgga
    19801 acagtaattg tagctggatt agttatagta ttgcgtggaa atccagtgat tgaggatgat
    19861 gaatttgaag atgatgattt tgatgaagca gagtgggaat cttaaaattt aataactatt
    19921 taagacgtgt tataagctat attcaatatg aatcttatac taaataaagt gttaaatatt
    19981 cagtttagac ttaaatataa aagataaaga acagaggaga ataaatgata aaagcacata
    20041 ttgttaatca tacacactgg gatagagaat ggtattttac ttctggagat gcattagtac
    20101 ttagtgaaca actatttaca gatgtaattg atgaattaga acgtaatcca gatgtaagtt
    20161 ttgtacttga tgggcaactt tctattttgg atgattatgt acaattacac aaagaaaaaa
    20221 tagaaatcat taaaaagtta ataaaagaag atagactaca tattggacca tggtttactc
    20281 aaactgatgc attctttgct agaggagagt ctattttaag aaacttaatg attggaattt
    20341 ttgaaagtaa aaagtatggt aagtatatga aaattgggta tttgccagat acatttggat
    20401 ttaatgcaca gatgcctata ctattaaagc atgtaggtct tgataatata ttcttttgga
    20461 gaggtataca tcttggtaaa caggttcaat caccatattt taagtggaaa agcttaaatg
    20521 gagaaagtta tatatatgca gtaaatatgc cacatggata tggaactggt atgcttttag
    20581 agccttcatt aaagtatgtt aatggacgct tagacccagc tattgatttt attaaatcat
    20641 atactgatgt agatgaggtg ttaatacctt ctgggaatga ccagttaaat ataataggtg
    20701 atttcaaaaa taaaattaaa gcgattaatg atatcggaaa gtattcatat ataacgagtt
    20761 catatcaaga gtttttaaaa tatataaagt ccatagaatc actagaaagc tatagaggtg
    20821 aatttagaga acctgtatta gcaagaatac ataaatcaat tggttctagt agaatggata
    20881 taaaattagc atgtgataaa ctagaaaaca agctgattaa aagaatagag ccattattag
    20941 taattgctaa aaagagtaaa attgaaataa gcaatcagtt acttataaat acatggaaaa
    21001 aacttttgga aggacaagca cacgatagtt tagctggatg tgtaactgat actgtaacag
    21061 atgatattct tcaccgtata agagaagcaa atgaaatatg tgacagtatt gaaaatacta
    21121 ttgtaaaaaa gatttcagaa gggcttaaat tatctaaaaa tgatatttta gtattcaata
    21181 cagaagctaa gcgttttaat ggatataaag agatacaagt tgtaagtgat agtaaaaata
    21241 tttattttaa agataattta gatgctacta taattgaaga aacttatgta aaatcgagag
    21301 aaaatgtatt agaagaaaca cctgctggca atatatttat tgaagaacca ggatattata
    21361 tattaaaagt aagaataaat atacagcttc ctgcacttgg ttataaagtt gttagttttg
    21421 aatcaagtga taaagaaatg gtgtcacttg ataaatctaa tgacactttt atatctaatg
    21481 ataattataa gataatatat gaagatggaa gcttaaattt acaacttaaa gatggaactt
    21541 acatatcaaa ctttttaaca ttaaaagata gtggtaatgc aggtgatacc tatgactttt
    21601 ctccactaaa aaatgatgag gatataaaac tttcgtttga taaagtgtat acagaaaaag
    21661 ctttaggata tgaaaaaatg gtggttaaag gtactacttt actaccactt acattagaag
    21721 atagaaaaaa caaatatctt aatgggaaat taaatgtaaa agtaagtatt gtcttatcca
    21781 aagaaaatcc attgatggat gtaaaacttt gtgttgataa cacgatatat aatcatagac
    21841 tacgtgttca tataaaaact gatataaaag ataataaaaa catagcatca ttgccatttg
    21901 gatatataac aagagaaaat ggagttttag acaactggga agatatttac agtgaaatgc
    21961 ctatagattt agaacctttg gaaagtaata ttacgcttac aaaccaaaat agaagttgta
    22021 ctgtatttac tagaggaata aaagagtatc aacatattga agatgaaatt gcattgacct
    22081 tacttgctac tacagatgaa ctaggaaagc ctgatttatt atatagacca ggacgtgctt
    22141 ctggtgatac aactaaaaaa ggtcatataa gaattaaaac agataaagct caaatgctta
    22201 aggaattaga attttcattt gcaatatata tggattctaa atcatttgat gaattggaaa
    22261 ttgcgaattt aacccacaat tatctaaggg aatgtgtaaa ttatcaatta caggattaca
    22321 atttcttttt atatcgtata gataataaga ttcaaaaatc aattgtcaat aaaactgtaa
    22381 gtagagaatt agaaataata tctttaccag aaaactatct tatatcagca tgttatcctt
    22441 cttattacag taaagataaa tttataataa gagtggaaaa tccaagtagt aatagaatga
    22501 tattggatga gcaaatattt aaaaatagaa atgggaaaat tgtaaatgca atagaagatg
    22561 tagaagagga acaggtatat gaaatagcac cttttgacgt tatttcaata ttattggatt
    22621 tataagtgta aaagtaagtt tattgtttaa agtctgacag atattaatct gccagacttt
    22681 ttaatttgta taaatttggg taaatattaa tgaatggttt tagtatttaa gtatgaattt
    22741 agtatttaat atagaattta ttgaagtaga ataattagtt taacaggata agcagtttaa
    22801 ataaagaatt attatattag agtgatttta tatataattt aaaataataa aattttaata
    22861 aaatgagtta aagattgtat attaatagag ttgatgtagt tacaaaaaag ttaccaattg
    22921 ttatgtattt acaaaactga tatattaatt acataaaatc taaaatagtt tgtaatagga
    22981 gtatatataa taaaaatttt aattaaataa agctaggtat atttataata ttaataaata
    23041 tattgctata aaagtttgtt taataaatta aataaagaaa agaggagaat aaaatgtaca
    23101 acatagatga tatgatatat aattgtccag tagaagcttt atcaaatatt ttaggtaaaa
    23161 aatgggtggc tataattata tggaaaatac aagaagataa aaaacgattt ggagagctac
    23221 aaagagctat accagactgt agtaagaaaa tgttagttca acaactagat tttttaattg
    23281 aacaggggat tatagtcaac caaaaaaagt tggttaataa tgttgtagag tcaacatatt
    23341 ttttaagtga atcaggatta cgattgcttc ctgtcatgga aaaaatgatt atgtggagca
    23401 ataaaaattt aaattgcgat aaatactaaa atttactctg taagtaatga aaatttaatt
    23461 aagctatgag tagaaaaatg tattaaaatt acataatttt ttagttagat tttaatttag
    23521 aaataggatt gtaatattgg aaataaaata aggaggaatt ttaatttgca aaatgtaaat
    23581 ttatttttag tatttataga aggtatagta tcattctttt caccatgtat actgccaatt
    23641 ttgcctattt atttgagtat attgtcaaat agtagtgtag aaaatttaaa agaaggaaag
    23701 acgagtttta taggaagttc tttatttaaa aatactatat tttttgctct tggaatctca
    23761 acaacattct ttatattggg ttcatcagta aaagtattga gtatgttttt taatgaaaat
    23821 aaagatttaa taatgtttat tggtgggatt ataattatta tcatgggatt attttacatg
    23881 ggaataatta aatcttcaat attgaataga gaaaaaagat ttaatgttaa gtttaaagaa
    23941 atgaaagcaa taacagcttt tatattaggt tttacgttta gttttggatg gacaccttgt
    24001 attggtccaa tattggcatc tgtattagtc atggtttcaa gttcaagtaa tcatcttagt
    24061 gccaatcttt taatagcagt atatactatt ggttttattt tgccattcat aattacagct
    24121 atgttttaca gtaaactatt taaaactata gataagataa aatctaacat ggaaattatt
    24181 aaaaaaatag gtgggattat acttatagta tcaggaatat taatgatggt aaatggattt
    24241 ggaagtataa gcaaacactt taatacgtca caaaatagta aaattgaaag caagcaagaa
    24301 gaaaataagc gagaaaattc tactgataaa gaagaaaatt ctgatggtaa tgatagtcaa
    24361 aaagattcta ataatgacaa taatgataaa gggtctaatg atgaagatag aataaagtct
    24421 attgatttta ctttaacaga ccagtatggt aaaacacata aattaagtga ttatgaaggc
    24481 aaagttgtat tcttaaattt ctgggctaca tggtgtcctc catgtaaaga agaaatgcct
    24541 tatatagaac aactttataa agactataat aaaaataatg atgatgtagt aattttagga
    24601 gtagcttcac ctaatttagg tagagaaggt tcaagggaac atgtagttaa cttcttaaaa
    24661 gaccaaggtt atacattccc tgttgtactt gatgaagatg gagcattagc atatcaatat
    24721 ggaattaatg cttttccaac tacatttata attgacaagg aaggatatgt aactcagtat
    24781 attccaggag ctatggataa ggcaactatg gcatcattta ttgaaaatca gagaaataaa
    24841 taaaagtatt taagtataag tttataaatc aatttaaggt tacaaaaaag tgcctaattg
    24901 taccacctaa ataatcttgt tatagtaaaa gtataaacaa tacaagaaag ggttgaattt
    24961 tatatgaaaa aaacatttga tgctataata ataggatttg gaaaaggtgg aaaaacatta
    25021 gcaggggatt aaaagtagct ttaatagaaa agtcaaacaa gatgtatggt ggaacatgtg
    25081 taaatgtggc atgtatacca acaaaaagct tagaaaattc agcaaacagc gtaaaaacaa
    25141 agaatataaa ttcttgggat gaagtacaag ctgaatatga gaaagctata gataaaaaag
    25201 aaactttaat tacaaaactc agagaagcta actataataa attaaattca aatgagaatg
    25261 taactatatt cacaggaatg ggaactttca ttgatgaaaa aacagttcaa gtcaaaactg
    25321 aaactgaaat ttatgagtta gtagcagata atatatttat taatactggt tcaagaccat
    25381 ttatacctaa tattaaggga attgaaaata agaatatagt atatgatagt gagtctctta
    25441 tgaatctaag aactttacct aaaaaaatga caattatagg agctggattt ataggtttag
    25501 aatttgctgg tatttatagt tcatttggag cagaagttac aatattaaac tctaataatg
    25561 gcattttacc aaatgaagat gttgaggatt cagaagaaat aataaaatta cttgcaaaga
    25621 gaaatgtaaa aattgttaat aatgcaaata taaaagagat taaagaagtt tctgaattag
    25681 caatagtaga atatgaagta gatggtaaat ctaaagaatt aacaagtaat atgatacttg
    25741 tagctacagg aagaaaagca aatactgagg agctagggct agaaaatgca ggtatagaat
    25801 taaatgaaag aggatttatt aaagtctcag aaactcttaa gactaataaa gagcatatat
    25861 gggctatagg agatataaat ggaggtccac aatttactta tatttcattg gatgattatc
    25921 gcatagttat taatcagtta tttggagata agactagaac aactaatgat agaaagaata
    25981 tcccaaattc tatatttata agtccagcat tttcaagagt tggtcttaat gttaagcaag
    26041 ctaaagaaaa gggatatgaa gtattagtgg caaagatgcc agtagaagca attccaagag
    26101 ctaaacagat tggcaaggca gatggattta taaaaatagt gatagataaa aaatccaata
    26161 agatacttgg agctagtatg atatgtgaaa attcaagtga gattatccat cttattcaat
    26221 tagctgtaga tttagaagta gagtatacat atttaagaga ccgtgtttat gcacatccaa
    26281 ctatgacaga agctcttaat gatattctat ctcctaatat gataaaagaa gtataacata
    26341 agtttaatat ataaacctaa aattatcatt ataagtagat tttagaaaga ggctatctca
    26401 aaaaatagag ataagtctct tttttactgt ttaaatgata aaaatatatt tgattttata
    26461 aaagaaga
//
LOCUS       DAEMHZ010000047        25142 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000047 DAEMHZ010000000
VERSION     DAEMHZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.047"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..665
                     /locus_tag="KPK72_001844"
     CDS             99..665
                     /locus_tag="KPK72_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG2489063.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            970..1431
                     /locus_tag="KPK72_001845"
     CDS             970..1431
                     /locus_tag="KPK72_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2489064.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEKEFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1499..3745
                     /locus_tag="KPK72_001846"
     CDS             1499..3745
                     /locus_tag="KPK72_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489065.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3738..5570
                     /locus_tag="KPK72_001847"
     CDS             3738..5570
                     /locus_tag="KPK72_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489066.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6289..7566
                     /gene="hflX"
                     /locus_tag="KPK72_001848"
     CDS             6289..7566
                     /gene="hflX"
                     /locus_tag="KPK72_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2489067.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7799..8296
                     /locus_tag="KPK72_001849"
     CDS             7799..8296
                     /locus_tag="KPK72_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2489068.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8346..8870
                     /locus_tag="KPK72_001850"
     CDS             8346..8870
                     /locus_tag="KPK72_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2489069.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9182..10546
                     /locus_tag="KPK72_001851"
     CDS             9182..10546
                     /locus_tag="KPK72_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG2489070.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEECEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10685..11851
                     /locus_tag="KPK72_001852"
     CDS             10685..11851
                     /locus_tag="KPK72_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2489071.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            11968..12750
                     /locus_tag="KPK72_001853"
     CDS             11968..12750
                     /locus_tag="KPK72_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG2489072.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(12869..13522)
                     /locus_tag="KPK72_001854"
     CDS             complement(12869..13522)
                     /locus_tag="KPK72_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489073.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13546..14910)
                     /locus_tag="KPK72_001855"
     CDS             complement(13546..14910)
                     /locus_tag="KPK72_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2489074.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            15568..16998
                     /locus_tag="KPK72_001856"
     CDS             15568..16998
                     /locus_tag="KPK72_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2489075.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            17101..18204
                     /locus_tag="KPK72_001857"
     CDS             17101..18204
                     /locus_tag="KPK72_001857"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2489076.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            18219..18455
                     /locus_tag="KPK72_001858"
     CDS             18219..18455
                     /locus_tag="KPK72_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2489077.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            18472..19521
                     /gene="vorB"
                     /locus_tag="KPK72_001859"
     CDS             18472..19521
                     /gene="vorB"
                     /locus_tag="KPK72_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2489078.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALSSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            19523..20263
                     /locus_tag="KPK72_001860"
     CDS             19523..20263
                     /locus_tag="KPK72_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2489079.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            20266..20805
                     /locus_tag="KPK72_001861"
     CDS             20266..20805
                     /locus_tag="KPK72_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2489080.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            20929..23028
                     /locus_tag="KPK72_001862"
     CDS             20929..23028
                     /locus_tag="KPK72_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG2489081.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPTNSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(23295..23810)
                     /locus_tag="KPK72_001863"
     CDS             complement(23295..23810)
                     /locus_tag="KPK72_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG2489082.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(23902..25113)
                     /locus_tag="KPK72_001864"
     CDS             complement(23902..25113)
                     /locus_tag="KPK72_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2489083.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSSLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         9655 a         2887 c         4397 g         8203 t
ORIGIN      
        1 aaataattta ataaatataa attaaattat atgtgtatat attgtacttt aatagtgata
       61 taataataaa aaaattttta aagagggtga tattacatat gagaaaactt gaagagatat
      121 tagaatataa taaatcattt gtagagaaca aagaatatga acaatatgtg acatcaaaac
      181 atccaaataa aaaaatagta gtcttatctt gtatggatac aagattaact gagcttttgc
      241 ctaaggctat gaatttgaaa aatggagatg tgaaactaat aaaaaatgca ggagctacta
      301 taatgcatcc ttttggaagt attatgagaa gtgttttagt tgcaatttac gaatttgatg
      361 tagatgaggt tatggttgta gggcatcatg gatgtggtat gtgtaatgtt gatacggata
      421 agttacttgg taaaatttta gatagaggaa tatctaatga tgtaatactt acacttcgta
      481 gtgctggaat tgatacaaaa caatggttac atggattcga ttctgcagaa gaatctgtaa
      541 aagatagtat tgatttaata aaagggcatc ctttaattcc agatggtatt attgtacatg
      601 gtctaattat atcacctgaa actgggaagt tagatgttgt tgtaaatgga tatgaagata
      661 agtagagtat cataatgtta tataatcaag atactgttga atatgatgtt gtttcaaaat
      721 ttattttggg acaacttttt tatttgaaaa tatatgaaaa ggtttatttt aagctttgca
      781 ataaaaaata taagtagctg actaattaaa aatcttacta ttatttatta aaattataac
      841 tatatgatat aaaaatattt aatacatgaa atttatatga attttttata aatatattga
      901 aaaaatgcat gcagtatagt aaaataatgc ttagctgact aagtaatata aaaaataagg
      961 agggcaagta tgaatagcaa ttctgaagaa aaattaaatg atgttttctt gcaattaatg
     1021 cgaatatatt ttataagaac tcatttttat tttgataaat taggcttata tcatggtcaa
     1081 ccccaaatac ttttttcttt aaagatgaaa gatgggcaaa gtcaaaaaga attagcagat
     1141 aaaaggaaag taaaagcatc tacaactaca gttatgataa agaggatgga aaaagctgga
     1201 cttgtggaaa gaaaacaaga tgaaaaggac caaagagtat ctaggatttt tttgacagac
     1261 aaaggtttag aaatatgtaa taaagttgaa gaatttaaca aggaattgga aaaagagttt
     1321 tttttaggat ttagtgaaga agaaaaaata gtacttagaa gacttctaat gcaagtacga
     1381 gataatttaa aagcaggatg tcataagcat gaagatgaaa aatattttta aagtttaaaa
     1441 taatgttttt caatttaatg ttattagact aattaatatt acagaaggga agaaatgtat
     1501 gtttaaatta attaaatatt taaagaaatc ggcattatca atagtaataa tagtctgtct
     1561 attggtaatt caagcggtat gtgacctttc tttaccagaa tacacatcca atatagtaaa
     1621 tgtaggtata caacaaggtg gagtagaaaa ctctgttcca tctgtgatta gagagagtga
     1681 attaaataaa attactttat ttatggataa aagtagtaag gataaagtac tagataatta
     1741 tactttgctg aataaaaagg attatgttaa gtataaagat aagtacccag gtctaaaaga
     1801 tgaatctctt tatgagttaa atactaaaga caaagacact atagatgatt taaatgttat
     1861 ttttggtaaa gctattttaa tagtatcagg gcttgagggc gatagtaaag aggtaaaagc
     1921 tatgaaagct cagttaatga gtaaacttcc accacaagct actcaaagtg gagatgttga
     1981 tatatttaaa ttgttatcag caatgccaaa ggaacagtta gatacattga ctaaagagat
     2041 gagcaaaggc tttgaaagta tgccagagag tatgataaca caaagctctg tttcatatgt
     2101 aaggtcagaa tatgagaaaa taggtattga tacagaaaaa actcaaaata attatatatt
     2161 atttactggt gctaaaatgc ttggtatagc aatgattagt atggtagcta caataacagt
     2221 aggattttta gcagcaagag tagccgcaag tgtaggtaga aatttaagaa gtggagtatt
     2281 tagaaaagtt atgtcatttt caaatactga aatgaatgaa ttttctacag cttcattaat
     2341 tactcgtagt acaaatgata ttcaacagat acaaatgttg atggttatgt tgcttagaat
     2401 agtattctat gcacctataa tggctatagg tggagtgata aaggttttaa acacaaatac
     2461 ttcaatggcc tggattatag cagtagcagt agttgctatc ttatcattaa ttgtagtgtt
     2521 attctcagtg gttatgccta agtttaaact ggttcaaaag cttgttgata agttaaactt
     2581 agtaagccgt gaaataatta ctggtattcc agttatcaga gcttttagta ctgaaaaaca
     2641 tgaagaagaa agatttgata aagcaaataa aaatttaact aaaacaaatc tttttgtaaa
     2701 tagagttatg acatgtatga tgcctgctat gatgcttata atgaacctta tcacagttct
     2761 tatagtttgg agaggtgcat acagtgttga ttctggagca atgcaagttg gagatatgat
     2821 ggcatttata caatatacaa tgcaaataat aatgtcattc ttaatgatat caatggtatc
     2881 tattatgctt ccaagagcat ccgtttcagc tacacgtata gatgaagtat tagtgtcaga
     2941 tataagcata aaagacccag aaaaatcaga ggcacaagag tttaaggaag ataaaaaggg
     3001 tcttgttgaa tttaaaaatg tttcgttcat gtatccagac gcagaagaac ctatattaac
     3061 aaatatatct tttacagcaa agccaggaga aacaactgcc tttataggaa gtactggtag
     3121 tggtaagtca actcttataa atttaattcc tcgtttcttt gatgttactg aaggagaaat
     3181 attacttggt ggagttaata taaaaaatgt ttctcaacat gatttaaggg aaaaaattgg
     3241 atatgtacct caaaagggag tattgttttc tggtactata gaatccaact taaaatatgg
     3301 tagaaaagac gcaacagatg aagaaatgag aacagcagct gaaatagctc aagctacaga
     3361 atttatagat tctaaaccgg aagcttttaa aactgaaatt tctcaaggtg gaacaaatgt
     3421 atctggaggt caaaaacaaa gactttctat tgcaagagca atagcaaaaa atccagaagt
     3481 ttatatattt gatgatagtt tttcagcact tgacttaaaa actgatgcag ctcttcgtaa
     3541 ggctttaaaa tcaaaaactg ctgaaagtac agttctaata gttgcacaaa gaattagtac
     3601 tatcttaaat gcagaacaga taattgtttt aaatgagggc gaaatagttg gaataggaac
     3661 acataaagag cttctaaaaa attgcgaagt ttatgagcaa atagctttat cacaactttc
     3721 aaaggaggag ctagctaatg agtagaatgg gaagaggtcc tatgggaaaa tctatgggag
     3781 caggacaaaa agcaaatgac tttaaaggga ctatgcgaaa actaattgca tatttatcta
     3841 aatttaaaat atcaataatt ctagtaattg tatttgctat tggtagtgcg tcgttttcta
     3901 tagtaggtcc aaaaatttta ggaaaggcaa ctacaaagat atttgaaggg ctagtgagta
     3961 aagtttctgg aggaaacgta ggtatagatt ttaatgcaat tggaaaaatc ttaacctttt
     4021 tacttttcct ttatttgata agtgcactgt tctcttttat acaaggattt ataatgagtg
     4081 gaatttctca aaaagtatcc tataatttaa gaaaagagat ttcagcaaaa ttggatagat
     4141 tgccaatgaa gtattttgat actaagacgc atggtgaaat attatcgaga attacgaatg
     4201 atatagatac tttaaatcaa agtttaaatc aaagtatgac acagcttata acatcagtaa
     4261 caactatgat aggtgttttg ataatgatgt tatctataag tggaattatg acattagtgg
     4321 cagttttaat attaccaata tcaatgtttg taatttcaag aataataaag aaatcacaaa
     4381 aatactttag atatcaacaa gaatatttag gaaatgtaaa tggtcaagtt gaggaaactt
     4441 acagtggcca aacaatagta aaagcattta atcgtgaaga tgaagtaatt gaagagtttg
     4501 ataaactaaa tgatagcctg tataattcag cgtggaaatc tcaattttta tcaggtataa
     4561 tgcaaccact tatgatgttt attggaaact tgggatatgt aatggtatca atcttaggag
     4621 gttggcttgc aattaagaaa acaattgagg ttggtgacat acagtcattt atacaatatg
     4681 taagaaactt tacacagcca atgactcaga ttgcacaggt tgccaattta ttgcaatcaa
     4741 cagcagcagc ttctgaaaga gtatttgagt ttttagaaga ggaagaagaa gtccaaattg
     4801 ttgaaaatgc agtttcaata gatggtttag aaggtaaaat tgattttgaa aatgtcaact
     4861 ttggatataa cccaaacaag actataataa atgatttctc agtaaatgtt aaacctggtc
     4921 aaaaagttgc tattgttgga cctacaggag caggtaaaac tacaatagtt aaactgttaa
     4981 tgagatttta tgatgttaat agcggttcaa tactgattga tggtcataat attaaagatt
     5041 ttaatcgtag tgagcttaga gaaatgtttg gtatggttct tcaagataca tggttattta
     5101 gtggttcaat tatggaaaat ataagatatg gtaagttgaa tgctacagat gaagaagtta
     5161 tagaggctgc taaatctgca catgttcata gatttattaa gacattacca gatggatata
     5221 agatgaaatt aaatgaagaa gcaagtaatg tttctcaagg tcaaaaacaa ttattgacta
     5281 tagctcgtgc tattttagct gatcctaaaa ttttaatatt agatgaagct acaagttctg
     5341 tcgatactag aacagaagta ttgattcaaa aagctatgga taatcttatg gaaggtagaa
     5401 caagttttgt tatagcccac agattatcaa ctatacgtga tgctgatatg attcttgtaa
     5461 tgaatgaagg agatatagtt gaacaaggaa atcatgagga acttctaaag aaaggcggat
     5521 tctatgccaa tctttacaat tctcaatttg aagaagatga agcaatgtaa caaaaaacta
     5581 tcttagcata gacatgtaat taataagata aaataaatga gggtgcctta aaatgaactt
     5641 tttaattcat aaggcactct tttgtgtatg aaataaagtg tatttttatc attttaacaa
     5701 tgaaaaagag gattatctat attttgagat agccacataa ttttatgtct ttacaaagtg
     5761 tttattttat tataatatgt agtaaggaaa atgttggagg tgattatcaa aatgtatatt
     5821 taattttatt ctttaaattt tattataaat agaatttaat tttaatagtt gatgattata
     5881 ttgaagtata tgtttttatt acttttatta atcatgtaaa taactactta aatttaagaa
     5941 tagggttaaa tataaaatga taattacata taagttaatt aaactaatgt atttttaata
     6001 tatatttaga attttaatcc ttcttatgat ataagtagct tgttttgtat tacttagatt
     6061 tgtaaggagt ttttttattg aaaaaactgc ataatatatt tgggaggagg tgtaagaaag
     6121 tgatttttta tataagattg agaaaataat gcacgattat ggttattata atagtaaata
     6181 agaagtgtca tttaaggata agataaaaag caaaattctt gatgaattta attatagagc
     6241 cataatctcg tgctatagat taaataaaaa atagcagata aggagaatat ggatatggcg
     6301 aaaaaaggaa taacagtagg attaaacata aataataaat ctgaggactt taatgaacta
     6361 atgattgaac ttgaaaactt atgttcagca tgtgatattg atgttgtagg gagtataact
     6421 caaaatgcaa agcaggttaa tagagcattt tacattggga caggtaaagt tgaggaaata
     6481 ttaaacttaa taaagaaaga aaatatagag atagttatat tttacaatga actttctaca
     6541 tcacaactaa aaaatcttga agaaaagctt aattgtgaaa ttatagatag aacggcattg
     6601 atacttgata tatttgcaca aagggcaaaa actagagaag ctaaacttca agtagaagta
     6661 gcaagtttaa aatatatgtt accaagactt ataggttcaa atgaaaattt aggacgtcaa
     6721 agtggtggag ttggtactaa aaatagaggt tctggtgaga aaaaattaga acttgaccgt
     6781 agaagaatag aagaaaagat aacatcttta aatagggagt tggatgattt aaaattccaa
     6841 agagaaactc aaagaagtat gagaagaaaa tcaaatcttc caaatgtagc tctagtcggg
     6901 tatacaaatg ctggtaaatc ttcaataatg aataaactag tagatatatt taaaaactct
     6961 gaagaaaaga aagtgtttga gaaaaacatg ttatttgcta cacttgaaac ttctgtcaga
     7021 aatatagttc ttgcaaataa caaagaattt ttattatctg atactgttgg ttttgtaagt
     7081 aatctaccac atgacttagt aaaagcattt aggtcaacat tagaagaagc atgtgaggca
     7141 gacgtactgt tacatgttat tgatatttca aatccaagct ataaaagtca tattaaagtg
     7201 acagaagata ctttaaaaca aataggtgca gatggtatac ctatgataca tgtgtataat
     7261 aaaattgatt taatagatgt agaagtattg gatagaattt tagatagtat cgataaagaa
     7321 ggtatatttg tttcagttaa aaaagatata aatatagata aaatgataaa atgtatttgt
     7381 gatagcatat ttaaagatta tgtaagatgt aagttcttaa ttccatatga taaaggtcat
     7441 gtagtttctt attttaacga aaatacgagc attataaata cagaatacag agaagatgga
     7501 gctattttag atgtagaatg tagcaatata gaatataata agtataaaaa gtatgcttta
     7561 gagtaaattt tgaaaaactt attatagcct aaaatatatt taatttataa tttattataa
     7621 attgattgta aatatgattt tatgaaacgg tatagccata atgctatgcc gttttgccat
     7681 ttataataaa aataaattta ttgacacaat agtaactaat tactataata taaagtaact
     7741 aattactatt taaaattatc aaaaaattaa aatctaagat aaagagaagt gtataataat
     7801 ggatgcagaa agtttaatga aaaaatatat ggtagatgat gatagtagaa aacaagctat
     7861 atttgcaagt atatttgtaa tgcaaaataa acttcaaact acatgtgata aattagaccc
     7921 agaattaaca atgaaacaat ggttactttt agctattgcg gactctgtag atgaaactct
     7981 tacattaacc aaacttggag aattaatggg gtgttcaaga caaaatgtaa aaaaactagc
     8041 tattgcttta gaaaagaaag gttttataga aattgagcaa aatgaaaaag attcaagagc
     8101 tatctgtctt gtgacaaaag atagagtgca agagtattct aaaaaagtaa gaggtatgca
     8161 ggaaaaagta ttacagcttt tatttgaaga ttttacagag aaagaagtgg aacagttatt
     8221 ttttggaatt atgaaattaa gtataggaat tgaaaatgtg aaaaattatg tagaaaataa
     8281 tgacgttgga gaataagcaa gtatcaaatc aactttgtat gacatcagag aggggggtaa
     8341 tttatatgaa aaatgtagta gtgatttata agagtaagta tggttcaaca aaaaaatatg
     8401 ctaaatggct ttcagaatta ttgtcttgtg atatttttga aactaagaat attaatatag
     8461 aaaaggttat gaagtatgat acgattattt taggaggagg tctttatgct tgtaatatag
     8521 cgggaatttc tttcttaaaa aaaaattata aagaattgca gaaaaaaaat ttagtagtat
     8581 ttggcgtagg agctgctcca tatgatgatg aagtaataaa gaaattaaga gaagtaaatt
     8641 ttaaggatga attaaacaaa attccttgct tttattgtcg tggggcatgg gatgagacaa
     8701 aaatgtcttt tatagattat actctttgta gtttttttaa aaaatctata gctaaaaaag
     8761 aacgtcaagg tcttaaacca gcagaagcac ctttattgtc tggagaagca tttgattggt
     8821 gtaataaaga aaatttaact ccaatacttg attatataag agaactttaa gaggtatata
     8881 tttataatta tttattatct aaagttagat ttcatagaaa cttactagac attatttata
     8941 aaataatatt attaaagatg gataatttaa ttgtttcaat aaggttataa ttttaaaaaa
     9001 tgtttatttt aaaataaatt attttataag cttacttata cttaataagg ggcttttttt
     9061 attggaagat ttatgttata ttgagatgaa tttattatga tataaaaaat aatgttagga
     9121 taaatttaaa tctttttcta aaaatgtatt aatataattc ttaaagaaag gatttttaat
     9181 agtggatata aaagagttag ttaaaatgca aagaaaatat tataatacag gtaaaacaag
     9241 agatatatcg tttcgtatag aacagttgaa gaaacttaag ttagttgtat cacaaaatga
     9301 agaaaaaata cttttagcac taaagaaaga tttaaataaa tcggattttg aaggttttat
     9361 gacagaggta ggcatgtttt atagtgaatt aaactttgct ataaagaata taagaaaatg
     9421 gagcaaaata aaaagagtta aatcaagtat ggttaatttt ccatctatat ccaaagttgt
     9481 tccacaacct tatggtgtta ctctcataat gtcaccttgg aattatccat ttcaattagc
     9541 acttatacca ttagtttggt cattagcagc agggaattgt gttatattaa aaccttctga
     9601 atattctgtg tcaacatcta gtgtagttaa agacatagtt gaagatactt tttcaaaaga
     9661 atatgttgca gttgttcaag gaagtcaaga agaatgtgaa aagttactat tggaaagatt
     9721 tgattatata ttttttacag gaagtactaa tgttggtaaa ataatcatga aatcagcatc
     9781 agagcattta actcctatta ctttagaact tggaggaaaa agtccttgta tcattttaaa
     9841 agatgctaat atagacctta ctgccaaaag acttacttgg ggaaaactaa taaatgcagg
     9901 acaaacctgc gtggctccag attatgtttt agtgcatgaa gatagaaaaa atgagttaat
     9961 agagaaaata aaatattata ctaataagta ttttggagat aatccatgta ataatgagca
    10021 atttcctaaa atcataaatc agagacattt taatcggata ttatctctta ttgataaaga
    10081 taagatagtc tatggtggaa attataataa ggagacatta aagatagagc caactatagt
    10141 agacaatgtt aattgggatg ataatataat gaaggaagaa atatttgggc ctatatttcc
    10201 cattttaaca tataaagatt tggatgaagt aatacaaaaa attatacaaa tgccaaatcc
    10261 attggcactt tatattttta caaaaaataa gtacctagaa aataaacttt tagaaatgat
    10321 tccagcagga ggatgttgta tcaatgatac agttactcat atagctacga attacttacc
    10381 ttttggtggt attggtgaaa gtggtatggg ctcatatcat ggaaaagctg gattcgatac
    10441 atttactcac tataagagtg tgttaaaaaa attaaatttg gatgtcccta ttagatatgc
    10501 tccatacgat aataaattaa ttaaggttct taaaaagata atgtaggtta tattgtagaa
    10561 taataaatat aaataaaatt gagtatttgt attctgaata tattttattt ttaatttggt
    10621 atatttgtta taaaagtggt gattttgtga taaaatttaa ttatatagaa ataaggagga
    10681 aaaaatgaaa attaattgga atacaaaata cacaacaata gcaatttata catttataat
    10741 agctgctagt agtattatat tttatctagt atcatcacaa attgatgtat tttctaataa
    10801 tttagatgct atatttacaa cattacaacc ttttataata ggatttgcta ttgcatatct
    10861 tcttaatttt attttaaagt tttatgaaga cagaattttt ataaaaagtg agaaattaaa
    10921 aaaactaaaa caaagttcaa aaagaggctt gggtctttta ttaacatatg caacagctac
    10981 attgatactt tacttattta tgcatttcgt attgccacaa gtaattgaga gtattgttgg
    11041 actagcaaat gatataccta tgtatgtaaa caatgcaact aagcttattg ataaattaat
    11101 gactgattta aatcttgatg agcaatactt taatttggca gttgataagt ggaatgagtt
    11161 tgttacatat attataaagt ttgtaacaga tttaatccca atattaggaa gtatgttaaa
    11221 gaatgtagcc tctagtatat ggaatattgt actaggatta attgtttcag tatacttatt
    11281 aattgataaa gaaaaattct atggattaag caaaaaaata acttatgcag tatttacaga
    11341 aaaacaagct gctagaattt tagaattaac tcatagaagt aactatacat ttggaagatt
    11401 tttaggtggc aaaatattag attcattcat tataggtatt ctaacatttg taatacttac
    11461 attagttaaa atgccatata cattattaat atcagttata attggtatta caaatattat
    11521 ccctttcttt ggacctttat ttggagctat accatctacg ctaatagtga tgtttgtatc
    11581 tcctataaaa gctttctggt tattgttaat tatattaata atacaacaga tagatggaaa
    11641 cattatagga cctaaaatac ttggagattc aattggtata tctgcatttt ggattttgtt
    11701 ctccttattg gttgcaggaa aattattagg ttttattgga atggttatag gagttccaat
    11761 gtttgcagta atttattcta taattaagga tattgttgaa agtaaattgg acaaaaaggg
    11821 attaccgaca gatacttccg attatatgta gctaaaaaat attatgtata attaaaaata
    11881 tataaattaa gcaatattta aagcactgag aaagttaaac tcttggtgct ttttagatat
    11941 aaaattatta tagaagaggt gttttttgtg gcatttatag aatgtaaatt taaatctaaa
    12001 tatactggag gagaaactga tataattgta attttgcctg tagcaaatgg aaaagaactt
    12061 tttagtggta aagagatata tgattttgaa ggtaaattta aaacattata cttatttcat
    12121 ggtttagaag atgaccaaag ttcatggatt agatatacta atgtagagca ttatgctaaa
    12181 aataaaaata tagccctagt aataccaaga gtagaaacta gtttttatac cgatatggcg
    12241 tatggacacc aatattttac ttttgtaagc gaggaattac ctaaatttgt tagagctata
    12301 tttcctttat cagataaaag ggaagataac tttgtagctg gtatgtctat ggggggatat
    12361 ggagctttaa aattagcgct ttcaaaacca aaagagtttt cggcagttgc aagtttttct
    12421 ggttcgcctg atatgattca tgagttagaa aatcaacaaa tagaaaccaa tgcagacatt
    12481 ctatttcacg attttggtag accagaagac gctagaaata gtgagaatga tttactttac
    12541 atacttaaga accttaaaga aagaaatgaa gatattccta aaatttatca attttgtggt
    12601 gatagagatt ttttatataa aaataatcag atgtttaaaa aatatgcaga ttctctgggt
    12661 gtagatgttg aatatgaaga gtgtgtaggg gggcatgaat ggcgtctttg gaatttatgg
    12721 ataaagaaat ttatagatat aattggatag tacacttatg gtattaacag gtatactata
    12781 aaatatcttt aaatatgata aagactgaaa tcctaaaatc aaattatgat tttaaaattt
    12841 cagtctctaa gaatttaata aaattttatt aagttcttac acgagttatt attggagtaa
    12901 aattgtcaac tatatctata tagtatttac tcaattcctc taaaatctct tcgcataaat
    12961 ccgtactatg accaagatta ataagaaact gttttgtaaa atgctttatt acagcttgag
    13021 aagatatatc gtacttgtgt gaagtataaa ctctaattat ttcaggtttc atagcaagta
    13081 taccaataca agaaagtatt aattcatcat gttctataga ttctttatca ttaacatatc
    13141 ttttcatgac catttcggtt acacttcttt gatagcttaa aatagcttct tcataagcta
    13201 tatccaaata aaaatttcta tatgcttcgt tttttagaag gaaacaaagg tcagcattca
    13261 tagcaagaaa gaacatcatc atagtacctt cttcatggta gttatagatt aaagactgca
    13321 tttcatttct tatatcttca tatattatag ctcctatttc gcttttactt gagaagtaat
    13381 agcttattag acctaaattt atattagctc tttctgcaat ttgcctagtt gttgtcttat
    13441 gataaccttg ctcaaaaaat aattctctag cagcacttat aatactagct tttgtttctt
    13501 tgcccttttc tgattttgac atatttactg tgtatacctc tcttttcaat ctttttttga
    13561 aaatttgcta actataaata ttgtaataga accacctatt ataggaacta aaattctgtc
    13621 aaatacagta agaataccaa tcatatttaa taatgccaat ataacactta ctataacagc
    13681 atagatagct cctttttttg tagcaccttt ccagcaaaga ccaattacaa atggtgcaaa
    13741 tattacacct gtacataatg gagctggcca aaacaataat tctaataaac tatcaaattt
    13801 aagtgctatg attgcaccta ttatagctgt caaagctgat gcaatttgag aatactttag
    13861 agtatttttt aaatcattaa attctatatt aggatgtaat aatttgttat aaatatctct
    13921 tgatataata gcactcattg aggtcattaa tgaatcggca gtagacatta tggcagccaa
    13981 tatagcagct aagaataata cagatattaa aggtggcaaa gtagtcgata tcatttgagg
    14041 taaaacctga acagatgata tatttggaaa taaactacca ccaatagctc caattattcc
    14101 tatagggaaa gcaagaaata atagtataaa tcctgcaatt acactacttt taactgcatt
    14161 tttagcatta tcagcacatt gatatcttaa aaaactttca taagaaaata ttgagcctaa
    14221 aactaatggt aaaactgtat acataaaatc actaatcgat ttatttcctg gaaagaatgg
    14281 ttcttttacc attgattgta acataagtaa agggttgtcg ccaactaatt ttaagcttat
    14341 ataagctact attggcaaaa acactataca aatagcaagt tgaataaatg ctgatactgc
    14401 aactccccac attcctgatg tataggtata tataattata actataactc ctataattac
    14461 agctatatca taaggaatac caaatgcagt agatacagaa ccaaaagaaa ttaattgact
    14521 acccataata gctagtatgg atattggtgt tacaagactt tgataagctc ttaaaaatac
    14581 actatgacca tatctagttt caatataatc agcaggtgtt atatcacaag ataagcttct
    14641 taattttcct gcgaaaaatg catttataaa gcaagctaaa gctgcaccta tagtagaata
    14701 cattccaacc caaagtcctc tttcagctcc ccattgaact ccaccaacta agcaccctcc
    14761 tccaaaagat gtagcagcaa tggttgcagc tagtataggc aaaggcagat ttctacctgc
    14821 gattaggaaa tcggatgctg ttttaacttt ttttgaaaaa taaaaaccaa tccatatgta
    14881 tattgcacac gtaacaatta aaagtaacat agtcttttct cctttataaa atattttaat
    14941 aaaatataca aaatatattt aaaatatatt ttatataaaa gtagtacgat atttgtttta
    15001 ttggttttag attaatatag ctataataat ttatgatatt agatggaaat tacgtaaaaa
    15061 tagaaaattg aaaattctga aatttttgca tattgatatt tatctcatag attctttttt
    15121 gtaattgtaa cacgctttta tttttaaatc aatagtacat gtataaaaat aaaaggaatt
    15181 taaataagaa aaagatattt ttaatatctg gatgtagtat agaaatatct tgaataaatt
    15241 attcttgaat aaaattataa aaaattaaaa tcactagaaa tatgataaaa taatttaaaa
    15301 tacgcatttc aatgacattt caaaacaaga aaataataaa aaacaaacct aaattaagtc
    15361 taaatagctt tttagttaat tttagtagta aaaaataaaa cttaaaaagt gtttgacaat
    15421 tatgaatttt gagaatataa ttaaattaaa attatacaca tataaaaata tattcctgaa
    15481 tcaagaatga gatatttaag attcaaaata tatattatta tacgtgaata ataaaaagta
    15541 agaaaatttt ataagggagg ggtagatgtg agtagtcaag aaagcaaacc aaaaggaaat
    15601 cttatatcaa attttagaga tgatatgaga gaccctaaga aaagagcaat atacacatat
    15661 tcaagttcta taaacttagc gtacaatttt ataggttact ttgtatcaac tgttacaaat
    15721 atttttttaa ctgatgtggc aaaacttggt gtaattatgg caggtggtat gcaaattata
    15781 caatctttca taaaaattgc tgtagctcct ataacaggta cagtatttga caaacaacca
    15841 ttcaaaaaag gaaagtatta tccatggctt agatatgcac cagcagcgtt agcattaaca
    15901 tatggaatat ttttcatagt tccattaatg aaaataagtc cagcattact tttacctttg
    15961 atgacagctt tattattaat agcatcttgt gctcagcaag tagttttcac tataatttat
    16021 gctatttacc ctgtagttgc taagacacct agagaccgag ttataggctc aactactacg
    16081 aatatattta aagaagttgg taaattctta gtaggttttt catatccgtt attattagtt
    16141 ttatttgcag atgtattagg tggagaagga atgggttatt taggtaccta cttattcttt
    16201 gcagcaggat gccttttagt attttggttt tcttcaacag agataataaa atctggagca
    16261 gaagaagaag ttcttcaaaa agaagagaag ccaaagatta aagcaagtga aatggcaaaa
    16321 gctttattca ctaatgtacc acttatgatg gcattttgtt tagaatttct tatatgtata
    16381 agatctatag gattaggacc tttggctcca tattatttta aatatgtaat agaagatgaa
    16441 cgtggattag cagtattttt atcaatcatg cctttggtaa gtgtagcatt catgtttttc
    16501 gcaccagtgt ttataaaaat ctgtagagag caaaaactag cttcaataat ttctttttct
    16561 atatgtgcac tatgccactt attggttgca tggactcctt gggggaaaac tactttagga
    16621 gtaactatgt tgctggcaat tggtggaggt ttttcaaatg tagtatcaat aataaatctt
    16681 aatttctttg ccggttcttg tgattatggg cattggaaat caggaaaaga tttgcctggt
    16741 ttaagtatgt cactatatcc agttgcaata caagtaggag tattacttgc aactactata
    16801 agaacagttt taatgaactc aatgggatat caagctgata tggtagtcac agaagcagtt
    16861 aaatcaggtt ttataaatat gatttcttat tctatggcaa tacctttaat aatagctgtt
    16921 gttatagcaa tactatatcc agtaagtgac aagaaattaa accaaataag agaagagtta
    16981 aatcaaagaa atgcttagtt aaaagaaagt taagtagatg gttaatttaa gctattaaag
    17041 taagtagacg caatagcgtt tatataaatg aaaatacata tacagagggg gcttttaaaa
    17101 atgatagaaa aattattgag aaataccgta aaagaattac atcaatatgt tccaggtgaa
    17161 ccaatagaaa aagttaagga aaaatatggt gtaaaagaaa taataaaatt agcttcaaat
    17221 gaaaatcctc taggaccatc accaaaagca atagaagcta tgattgaaat gttaaaacaa
    17281 ggtcaacttt atccagagcc agaagctaac gaacttagaa gaaaattagc tgaaaagcta
    17341 gatttaaaac cagaaaattt tattgttgct aatggtgcag ataacgttat cactctaata
    17401 ggtgaggctt ttataaatag aggagatgaa gttatatatt gtaacccaac atttccatca
    17461 tatagaactg caactataaa aaatgaaggg ataccagtag aagtaccttt aacagaagat
    17521 tataagtatg atttacaagg tatattagat aagattacag ataaaacaaa attaatatgt
    17581 gtatgtaacc ctaataatcc aacaggaaca attgtggatg ataaagagtt agaagaattt
    17641 ttaaagaaag tccctgaaaa tattataact atattggatg aagcatatat agaattttta
    17701 acagttccta attatgtgga tggacttaag tatgtaaggg aaaattataa cgttatagta
    17761 acaagaactt tctctaaaat atatggtctt gcagggctta gagtaggata tggtattgct
    17821 aaagatgaga taataagaac tttatttaca gtaaaagagc ctttctctgc aaatcgtgtt
    17881 gctataagtg gagcaacagc agcacttgat gatgaagagt ttataaaaga aacatatgaa
    17941 ttaaatagag ttggaatggc ttactttaaa gaagagttta caaaaatggg atttgatgtt
    18001 gtagattcac aatcaaattt cctttatgta gatatgaaaa cagatatacc taagttattt
    18061 gaagacttga aaaaaagagg atttgtaata agaccaagtg ctacacatgc acgtgtaagt
    18121 attggaacta tggaagaaaa taggaaattt gtagcagttt taaaggaaat attaaatatt
    18181 aaaggagagc caagtattgg ataatagata gaaatattat gaaacggggt gtagatatga
    18241 acttaaaaat aaacgcagag agatgtaaaa gttgtgaata ctgcgttata tcttgtaaaa
    18301 aaggagcatt aaaaatatca agtaagataa acaaagaagg ttatgctcat gtagaagttg
    18361 atgagagtaa atgcatactt tgtggaattt gctatcaggt gtgtccagat aatgtttttg
    18421 aaataattaa agaagaagca tgtcaagagg cttagaagga gttgggataa tatgaaaaag
    18481 atgtggaagg ggaaccacgc cattgcagaa gcagccctaa gaggaggttg tgaattttat
    18541 gcaggatatc caattacacc acaaacagag gtaatggaat ttttatcaca tagaatgtct
    18601 gaacttggaa gaacttttat acagtccgaa aatgagatgg ctgctatata tatggtatat
    18661 ggagcatatg caagtggaat gaggtctatg acgagttctt caggaccagg aatatcttta
    18721 aaacaagaag ggatttctta cttatgtgca aatcattacc cttgtgtaat cgtaaatgta
    18781 cagagatggg ggcctggtct tggctcattg gactcagcac agacagacta tctaagagat
    18841 actagagggg gaggaaatgg agattatcat ttgatagttt atgcaccaaa cagtattcaa
    18901 gagactgtag atttgatgta taactcattt gatgtagcgg agaagtacag agtgcctgtc
    18961 gaaattttga cagaagcagc tttaggtcaa atgatggaac cagtagaatt tccagagttt
    19021 aagaagagag aaggagattt aggttggact tatgatggaa gtaatagaga ccatgcaaag
    19081 gttgcagata accaaaagcc aactttttgt atggagaaac gtatgagaat aactgaaaat
    19141 gaacaacaat gggaagatta tcaagtagaa gatgcagaat atgtgtttgt agcgttcggt
    19201 ataccatcaa gaacaactat gaatgctgtt agaagactta gagaacaagg tgaaaaagtt
    19261 ggaataataa gaccaataac agtatggcct ttcccatata aagcatttga aaaggtaagt
    19321 aaagatgtaa aaggatttat ctcagtagaa tcaacagaca caggtcaatt agtagaagat
    19381 gttgctttat cctcaaagaa agtatgtaaa gaaaatgtac ctgtgtatgg tttatttagt
    19441 ggaaatcata taccaaaaac attgcaagta atggacactt atagcaaaat aaaatctggc
    19501 gaaatcaagg aggtgttcta atatggctac agaaaaatta acacctagcg ttgtttcaat
    19561 accaaacaag ttttgcccag gttgtggtca tggaatagta aatcgtataa tagcagaggt
    19621 aatagaagaa catggatatg aaaaaaatca tgtattaaca ttaggagttg gttgtgtctg
    19681 caatatgaat tttagctgga atggtgataa gatgcaaact gcacatggta gagcttcatc
    19741 tacagctata ggagttaaag tagcacttcc agatacatta gtaatgactt accaaggtga
    19801 tggagatgct tatgttatag gtctttctga aactttaaat acagcatata gaaatcataa
    19861 tgtgactgta tttgtaataa acaataataa ttttgcaatg actggtggac aaatgtcatg
    19921 gaccactatg ccgggtcaag tcacaacaac aagtgtaaat ggaagagata taaagactac
    19981 tggtagtcca ctgaaagttc ctgagatggt ggcaaacttc tttgatgtag catatgtagc
    20041 aagaggttca gttcatagcc caaaggaaat aattaatcta aaaaaatata taaaaaatgc
    20101 aatagaagct caattaaatg gagagggata ttccttagtt gaaatattag caccttgccc
    20161 aactaactgg ggtgtatcac tagagaaatc tatcaaatgg atggaagaag aaatagtgcc
    20221 atattatgct cttggcgaat ttaagcaaag ggatggtgaa tagttatgga aaaagagcta
    20281 gtatttgcag ggtttggtgg tcaaggagtt ttaactcttg gtctgattgt agctgagtca
    20341 gcacttgaat tggggaaaca agtaacttgg atgcctgcat atggtccaac tatgagaggt
    20401 ggaaaagcct atagtgtagt aaagttttct gatgagagta taggtgggcc tgatatggaa
    20461 gaaatagatg ttttagtagc aatgaacaaa ccatctttag attatataaa tttagttaaa
    20521 gaaggtggaa cagtagttat taatacatct gcaatagatg agaatgtaac attaagagaa
    20581 gatataagtg tagttaaaat aaattgtcag gaattagctc aaaaagtaaa caatccaaaa
    20641 gcagcaaata tagtagtact tggagcatta attgctaaaa ctgggttatt tgaaaaagaa
    20701 ttagctttaa aaactatgtg tgatttcttt gaggaaaaag gaaaaggaaa gtttaatgtt
    20761 caaaatgaag cagcattcat ggaaggctat aataacgcca tttgataaat tacccttatc
    20821 ttaagtgatt tatctcaaga tttaataatc tttagtgcca agagaaactt catcttgaga
    20881 taaaattcac ttaaaatcac aactataaac atataggagc gtgaatttat gagtttggaa
    20941 aaacttatta gaccaaaaag tattgctata gtaggagtga ctgataaact tggttttgga
    21001 agaagtgcag cacttagtat agtaaaaagt aaggaaacag atagagtata ttatgttaat
    21061 cccaaaagag aagaattgtt tgggagaaaa tgttataaga cgatacaaga agttccagaa
    21121 gtagtagact gtgtagtagt atgtacgcct agaaatgtag ttccatctgt attaaaagat
    21181 tctggagagt taggaacaaa ggcagcagtg gtatatgcta gtggatttgc agaggaaggt
    21241 acagaggaag gtacagattt agaaaatcaa cttatagaaa tatcaaatac atacgatatg
    21301 aaaatattgg gtccaaactg catggggttg ctaaattgta tagataaggt taatctatgg
    21361 gcaggaggtt ctaaatggga tttagatact aaaaaacctg gtataggaat tgtaggtcaa
    21421 agtggattta taactgctga aattgtttca agtgattatt ttaatatatc ttatggtttt
    21481 tcaacaggaa atggaaatat agttacacta gaagatgcag tagatttcct tgtagatgat
    21541 gattatgcat cagtaatagc aatttacctt gaaggtttaa aaaatcctca aaaatttata
    21601 gatgctttaa aaagggcagc acaaaaaaga aagccagtaa taatattgaa atcaggaagg
    21661 tcagaaaaag gtgctatttc tgctgcttct catacaggaa atctagctgg ttcgagtaaa
    21721 gcttttgaaa gtatttttga aaaatatgga gtaatttctg tggaaaattt agaacagttt
    21781 atgtgtttag cacaagcttt tagtgtttta gatggtaatt tacctacaaa ttctaatttt
    21841 gcagcaataa acttctcagg aggagaaaat actatttgtg cagatttagc agaagaaaat
    21901 ggtgtagaac ttgcagaaat atcttctgaa actaaagaag aaatgaaaaa atatcttcca
    21961 ggatttgcaa cacctaaaaa tccactagat gctacaacag cattattcca tgaaaaggat
    22021 atgatagtag gtttattaca tacctttgaa aaggatgata gtgttggatt tactatggta
    22081 ggtgcaaata taagagatga agaaaatgaa atgcatgaaa ccctttgtaa agctgtaagt
    22141 gaagctagag aacaaggatt gaaaaaacct gtgtttgcag ttcctacact tgaaggaaat
    22201 agatatttag attataggac tagattagaa gataatcaag tgcctataat gtcatcagtt
    22261 ggaactacat ttacttgttt taataaaatg gctaagttta tagattatga ttattcaaaa
    22321 agaactttag aatttaaagc tgttaagaaa agagagtcaa ataatgtagt agcattatca
    22381 gaattagata gtaagattga gatgaaaaaa tatggagtac cagttccagg acaaggtaat
    22441 gcaaaaagta ttgatgaatt agatgaaatc ttaaaatata ttaagtatcc tgttgtttta
    22501 aaaataaatt cttctgaaat attgcacaaa tctgatgttg gaggagttaa aataggaatt
    22561 aagaatagag atgaggcagt agatgcatat aatgagattt taactaatgt aaaaaaagca
    22621 aaaccagatg ctaatattga tggtatatta gttcaagaaa tggtagaaag tggtatagaa
    22681 attattattg gtataactaa tgatgaccaa tttggaccaa tgttattagt cggtttagga
    22741 ggagtatttg tagaggtatt taaagatact acattatatc cattacctat aaatcatgat
    22801 gaagctatta tgatgcttaa aaaactaaaa tcatttaagc ttttaaatgg ctatagagga
    22861 agtgagcctt gtgatattga tgctcttgca gatatgatgg tcaaattagg aaaatatgct
    22921 tatgaaaata aggatgaagt caaggaaata gacttaaatc cagtgtttgt atatcctaaa
    22981 ggaaaaggcg tatgtgcagt agatgcctta atagttaaat ataaatagtt tataagtttt
    23041 ggtattcaac cataattaaa gcaccgactg aaaatcacat tttgtaagtt agatagtgtg
    23101 attttcagtc accccaaatt taatttaaaa atattttaaa atgaaagtat agttttaagt
    23161 ttcattgtga aatagcactg tataaacaat aaaatactac gtataaaacg aataataaaa
    23221 ggtgttttaa tgataagaat caatcttgat tttcattaaa acaccttctt tattatatta
    23281 aaaattttta aacgttatat tgtcacaggt gggtttgttg gagctgcata tgtacgatta
    23341 gcaaattctt ggtcaacaaa gaatagacca ccctctccag caagtttaac acgttttata
    23401 ttgtcttcac aatttgtctc ttcttctgct tgttcagata taaaccaaag taagaattga
    23461 gaagttctaa agtctgattc agagtttgct acagctgcta agttgtttat taaagctgta
    23521 accttttttt catgagctaa cgtacgctca agaatatcca ttgcagaagt aaagtctgaa
    23581 tctggcattg ggatagcatc aagctgaacc tttcctccta cattatgtaa gtatttgtaa
    23641 aaaaacatag cgtggtctgt ctcttcttta tattgaacat aaaaccaatt agaaaatcct
    23701 ttaagtcctt ctgcttcaag gtaagaagac atagaaagat aaagatacgc agaatataat
    23761 tcatggttaa tctgctcatt aagtaatttt tccattttag ctgaaatcat ttttatacct
    23821 ccaaatttta aatgctctta taaaatataa taatattaaa tggaaaaata ttcaataaaa
    23881 gtgtttaaat attttacata tttaataaaa atttatacta ccattaaaaa aattataact
    23941 tacaatggct atagagccaa ttaatggtgc atttgctcca aaatttgaat gccttacact
    24001 tattttttta tatttagaaa aacttgctga agcagtcaat ttttttaata atgtattttc
    24061 tataaatgta ccaggtatat taaagtcata gcctattatt atagaactac aatcaatcaa
    24121 tgtaagtgta ttaactaatg catatgatac gtatgtacaa aattcatcaa gcaaaaacat
    24181 ggcaagacta tctttattat ttgcctcatc tattaacttg actaaagaaa aatctttaca
    24241 gttaattaat gaggagttag gataaatatg gctcaatgat tcaattcttt taattagatt
    24301 actgacactt gtataataat ctaaacatcc attgttacca caactgcaca atggtccact
    24361 aaaattaatt gatgtatgtc ctatttctcc actttgtcct aggttgccag tatgaagttt
    24421 attttctaga actagaccag ctccaatacc attcattatg tgtaggtata tataatttga
    24481 tatattttta cctaaaccat acattttttc tgctaatgca cctgaattag catcatttat
    24541 caaaaatatt ggcaaatcag acatttcttt tataaaatct actatattta ggtttgatac
    24601 atttccaaaa tctgggggat ttagtatggt accatttata tcatctactg gacctattga
    24661 agaaatacca attccaatta tatctctctt gttgattttc attattttag taaacattct
    24721 ttttattata tcttttaaaa catcatttga tagacatttg gggtaagttt cagaaagttg
    24781 ttttacaata ttacctttta aatctgaaat aattacctta catatatttc ttcttattag
    24841 gattccacaa atacaaggcg atatattaga aatatctaat aaaataggtt tacgacctga
    24901 tgaatgtgca tttaaagttg atgactctat ttccctaatc atattttcat taataagttc
    24961 agtaactaga tttgataaag tcattttagt aagtcctgta gacttagcta aatcaactct
    25021 ggacataggt gtatttgtag aaagtaattt caatattatt aacttatttt taatttttac
    25081 atctttgatg ttagaacctt gtttgttact catcataaat ctccttaaat aaagttaagt
    25141 gt
//
LOCUS       DAEMHZ010000048        24275 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000048 DAEMHZ010000000
VERSION     DAEMHZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.048"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            112..963
                     /locus_tag="KPK72_001865"
     CDS             112..963
                     /locus_tag="KPK72_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG2489084.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKNFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1096..2709)
                     /gene="ggt"
                     /locus_tag="KPK72_001866"
     CDS             complement(1096..2709)
                     /gene="ggt"
                     /locus_tag="KPK72_001866"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG2489085.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2934..4871)
                     /locus_tag="KPK72_001867"
     CDS             complement(2934..4871)
                     /locus_tag="KPK72_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489086.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEVL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLLKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKKSVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNFRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4858..5625)
                     /locus_tag="KPK72_001868"
     CDS             complement(4858..5625)
                     /locus_tag="KPK72_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489087.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGYIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5913..6587
                     /locus_tag="KPK72_001869"
     CDS             5913..6587
                     /locus_tag="KPK72_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489088.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6611..7615
                     /locus_tag="KPK72_001870"
     CDS             6611..7615
                     /locus_tag="KPK72_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2489089.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKILDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7727..8956
                     /locus_tag="KPK72_001871"
     CDS             7727..8956
                     /locus_tag="KPK72_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2489090.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNNQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8964..9638
                     /locus_tag="KPK72_001872"
     CDS             8964..9638
                     /locus_tag="KPK72_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489091.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9641..10873
                     /locus_tag="KPK72_001873"
     CDS             9641..10873
                     /locus_tag="KPK72_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489092.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11159..12424)
                     /locus_tag="KPK72_001874"
     CDS             complement(11159..12424)
                     /locus_tag="KPK72_001874"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG2489093.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12477..13625)
                     /locus_tag="KPK72_001875"
     CDS             complement(12477..13625)
                     /locus_tag="KPK72_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2489094.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13725..14270)
                     /locus_tag="KPK72_001876"
     CDS             complement(13725..14270)
                     /locus_tag="KPK72_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2489095.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14493..15437)
                     /locus_tag="KPK72_001877"
     CDS             complement(14493..15437)
                     /locus_tag="KPK72_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2489096.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15823..16704
                     /locus_tag="KPK72_001878"
     CDS             15823..16704
                     /locus_tag="KPK72_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2489097.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16765..17973)
                     /locus_tag="KPK72_001879"
     CDS             complement(16765..17973)
                     /locus_tag="KPK72_001879"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG2489098.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(18046..18298)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18450..19439)
                     /locus_tag="KPK72_001880"
     CDS             complement(18450..19439)
                     /locus_tag="KPK72_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG2489099.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            19591..20031
                     /locus_tag="KPK72_001881"
     CDS             19591..20031
                     /locus_tag="KPK72_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2489100.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            20358..20585
                     /locus_tag="KPK72_001882"
     CDS             20358..20585
                     /locus_tag="KPK72_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2489101.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            20611..22731
                     /gene="feoB"
                     /locus_tag="KPK72_001883"
     CDS             20611..22731
                     /gene="feoB"
                     /locus_tag="KPK72_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2489102.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(22864..23682)
                     /locus_tag="KPK72_001884"
     CDS             complement(22864..23682)
                     /locus_tag="KPK72_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2489103.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            23889..24173
                     /locus_tag="KPK72_001885"
     CDS             23889..24173
                     /locus_tag="KPK72_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489104.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
BASE COUNT         8549 a         3644 c         3068 g         9014 t
ORIGIN      
        1 atttctaaag atttatttac ttatgtaata aattataata atattagttt ataaagttaa
       61 taattaatat aaattaaaat aaagataata taaacatagg aggtacattc tttgaaaaac
      121 attatttcag caaaagaact tatatcaaaa ctagaaaaaa atgataatct agttgtaata
      181 gattgcagat ttgatcttat caatagaact tatggtattg attcttataa aaaaggacat
      241 atcaaaggca gttttatctt agatatagac aaggatttat catctcctac aaaagaacat
      301 ggtggtaaaa atcctttaca agacccttta atactaaaag aaaaattaga aaaaatgggt
      361 gttgataatg acactacgat tgtaacttat gatgatggag atttaaatgg agcttgcaga
      421 ttatttttcc aattaaaaca tttggggctt aaaaatgtct atgtactaga tgggggaata
      481 acatctttcg taaaggaagg tggagaattg gaagagaaag tgaatatacc aagttgcaca
      541 ggtaaggaaa ttagaccaaa tataaacaat gatttagttg ttccaatgga atatgttaag
      601 tctaaattat acaaaaaaga tactgtaata atagattgcc gtgctaatga gagataccaa
      661 ggtttagttg aacctgctta ctcaaaagct ggtcatatac caagtgcaaa aaattatttt
      721 tgtaaggatt taataaaaag tgactttgaa aatggctcac ttaaagatat agaattttta
      781 aaaaatttct ttaaagattt aaataattat gatgaagtaa ttttatcttg tggttcaggg
      841 atatctgctt gtgttaacag tcttgctctt agagaactgg atatacctca caagatttat
      901 attggtagct ttagtgactg gattagttat gatgacaatg aaatcaaaac tggtcaagaa
      961 taaggctcat aatctatata aaatcagcat atgatagctt aagttttgta aaggcgtcag
     1021 tcattgtaga ctttcgcctt tacttattat ttcatatact ctttaaattt attatatata
     1081 ttttagttga taatattaat aaactgcaat atgaccatca catcttgact ccgtcccaca
     1141 aatataactt tcctgttctg ttttaaatat tatttgacct cgtcccatgt caacagtatc
     1201 atgaattact tttatttcat gacccttttc aattaatgaa tctattatat gctttggcat
     1261 ctctggttca acttctattt cttttccttt tatccactgc catcttggag catctagcgc
     1321 ctcttgtgga tttaatccaa aatcaatcat atttgttaac acttgaatat gtccttgtgg
     1381 ttgcataaaa gctcccatta caccaaatgg acctattgct ttatcttctt tacctaaaaa
     1441 tccaggtatt atagtatgat aaggtttttt aaatggtttt actacattgt ggtgtttagg
     1501 gtctaaatta aaattatttc ctctactatg taaagcaatc ccagttcttg gtatcacaat
     1561 tcctgaacca aaatttatgt aattgctctg tatatatgaa accatatttc catccttatc
     1621 tgcagtacaa aggtatactg taccaccaca gtatggctct cctgcagttg gatacaaagc
     1681 ctctttattg tcaattaata aacttctttt tttggcatac tcttggctta gtaattcctg
     1741 aatctttacc ttcatatgct ctatatcagt tacatatgtt ttagaatctg caaaagctaa
     1801 ttttaaactc tctataattt tatgtatgtc ttctacattt tctatatttc cttctaattc
     1861 caattctttt aatatgttaa gtgccattaa aactgttatt ccatgaccat ttggtgggat
     1921 ttcaaatatt ttatatccct tatattctgt acttataggt tctacccatg ttggataaaa
     1981 acactctaag tcactctttc ttatagcccc attaaatttt ttagaatatt catctatttt
     2041 atcagcaagt cttcctctat aaaagctttc agcctgagta tttgctatct cttctaatgt
     2101 acttgcctgt tcttcacata taaatatatc tccagcctct ggagctttac catctttaga
     2161 aaatgtatca aaccatggtt tgaactcttc tccaataagt tctttttcat agagttcata
     2221 tgattttttc cataccttag ctacattagg agatactacg tatccctctc ttgcatagtt
     2281 tatcgctgga gataagcatt ccattaagct aagtttacca tattttttat ttaattctga
     2341 ccaagctgcg ggtattccag gtacagttac tgctccaaac ccatattttg gcatttcttt
     2401 catattatta taatttttta attccatatt ttctggagca aagccactag aatttaaacc
     2461 atacaatcgt ttttcttcct ctatccaaac aagggcatat gcatctcctc caatcccatt
     2521 gcttgtaggt tctactactg ctaatgtggc ggctgttgca actgctgcat caacagcatt
     2581 tccacccttt tttagtattt caagtccagc ttgagatgct aatggtgttg atgttgcaac
     2641 tgctccattt ttagcataca cgacatttct tattgatgag tatttatgtt ggtaggcatt
     2701 aaacttcata aatttcagct cctcgtcatt ttttatttga tattctgctt agatttatta
     2761 tataacatca agtaatgtaa ctgatacaaa atgatataaa atatagactt tttcctaact
     2821 ttaattttat ctttaaataa atatcttaaa ctcaattcta gtattttcta tctgcactta
     2881 aataagttat tttattcaaa acatatatct aattcttttg acttaaaaat aatttattct
     2941 tttatcatgt ttagagcata ttttaaagtt aaaaaataat atataaaata tcctataaaa
     3001 taaatgacta gagttatgat aattggtaca agtaatgacc ttggtataaa cttttgtgct
     3061 atagacatag caaataaatt atgaactgta cctacaacta atggcattaa aaatataact
     3121 tttaactgtt ttgatattat tttctcaata tctttattat ttgctccaag ttttattaac
     3181 ataatatatc tttccttatc atcatatata tttgataatt gtttaaataa gattatacta
     3241 cctgtacata aaagaaatac tacagacaaa aatatgccta taaacagcat tagaccactt
     3301 aattttatca agttattata actttcaata cttgatgtaa aattaaaagg ataagtaaat
     3361 ttcatatttt cacttactat attttttaat tttaaagtca aatccagttc ttttttttca
     3421 ttttttatat caattaatct tattaatcta aaatttccat gtaattttaa ctgattaaat
     3481 acgtcatctt ttactacaat taaatcaaac gtagcttgtg aatttatttc tggctccaag
     3541 atgctttttt gtactttaaa cagatttata tcatactcat ttgtatcttt aatatgcaag
     3601 tttatttttt caatctcaaa gtctttaaaa aatatttttt tgtatgaatc agaaacatag
     3661 tacacggatt tctttgaagt taattcttca taattgttat tctgatattg tcttaatttt
     3721 ttaaaatcac tttctctaat aatatctaca gacgttctat cttctatata actatttcca
     3781 tttttataat acatttcaat atttatatct ttgtctataa gttctattgt tttatcaaag
     3841 attatcttat tatctttttt atatttatat aataatttgt caatttcgtt atttacataa
     3901 gaatttccta aatttatggt ataactgtat ttataatttt cacttatgtt cctatctata
     3961 tcgtaataaa gtgatatggt aaaccctaac atagttacac ttgtagctat taatattgct
     4021 attacagcca atacactact attgctcttt aatttaaatc caagttcaga aaatgcaatt
     4081 aaattctgtc ccttataata aaaacttttt ctcttcttaa cagcatcaat aatcatatac
     4141 atcgttgatg aaaaaaataa aaaagttcca ggtattattg taactaatat aaaaactgaa
     4201 agcataatat tttcagcaat aaaataacta attgacatta aataagaaat agctattaat
     4261 aaaacaccaa gtacacattt taactttata aattttttaa acttatactt ttctggatat
     4321 ttattaaata actctataat tctcttattt ctgataacta tagtatttct tattccaata
     4381 actataaata ttattaaaaa taccgctagt gtcttaacta aggcctttat acttaagttc
     4441 atatttacta ctatcatttc attcattaat tttaaaagca tcatagtcat aagctttgaa
     4501 aataacagtc ctaaaaatat acctattagt attgcaacta tacccatcaa aaaagtttca
     4561 tataaaaata tttttcctat ctgatttttc ctcattccaa gtaaactata agttcctact
     4621 tctcgcctac gtctattgaa aaaaaatgag tttgaataat atataaataa aaatacaaat
     4681 accactatta caattgaact tgtattaata ctcatcctag ttttttctcc aagtgaactc
     4741 aacacttcat tatactctat agatttaaaa ctaaaaaata caaatacact aaatacaatt
     4801 gaaataaagt acattaaata attaaaaaaa ctattcttta cattttgcat tgcaattcta
     4861 aataaagtca tttttattaa ctcctccaat taaggaaact gtatttacta ttttattata
     4921 gaactctctg ttactttctc cctttacaat ttctgtgaat attattccat cctttataaa
     4981 gagtattcta ttgcaaaaac tcgcacttat agcatcatga gttaccatca atattgtttt
     5041 attgctgtct ttgtttaatt ttaatagaca attcaacaaa tccctagcag atttagaatc
     5101 taatgctcct gtaggctcat ctgcaagtac taatgatggt tttgttgcta tagctctaca
     5161 tacagctcct ctttgctttt gtccaccaga aacttcgcca gggtatttat ctaatataga
     5221 ttctatattt aattcttttg aaatctcttt tattctattt tctattactg atactctttc
     5281 attttttaaa gaaagaggca acattatatt ttcttttatt gtaagtgtat ccaaaagatt
     5341 agaatcttga aatacaaagc ctagattatc acgtctaaaa tctgccaatt cattttgctt
     5401 cattttaata gtattctttc catttatata aatatatcct gatgaaggta aatctattgt
     5461 agaaataata tttaaaagtg ttgatttacc agcaccagaa gaccccataa taccaacaaa
     5521 ttctccttta taaaccttga aagttattcc tcttagagct tcatgttttg aaccttttct
     5581 tccatactcc ttttttatat tttcaacaga taatatttct tccatagtta ataatcacct
     5641 ttcatcacaa ttagttattt atcaattctc acaaatatat tatatatatt aaaatgactt
     5701 ttaaacattt aattatatta atttaaggtt acattttcgt aagtattaat tatatttctt
     5761 caaatttatt gcaattatgt ttaaaatata atttatcaag taaaatatag ttatattaat
     5821 ataattattt taggtaataa ggtagaaata ttttaattta tcaagtagaa tatagttatg
     5881 ttaatataac tattttgaat taggaggtaa gaatgtttaa aataatggta gttgaagatg
     5941 acgtctcatt gaaaaatatc atagcaaagt gtcttactaa atggggacat gatgttcatc
     6001 aaatagaaaa tcttgaaaat ataatagaag agttcaaaaa ctataaccca gaattagttt
     6061 tattggacat aaaccttcca ttttatgatg gttttcattg gtgtaatgaa ataagaaaaa
     6121 tttcaaaagt tcctataatt tttatttctt caagaaattc taatatggat gtaataatgg
     6181 gtgtaaattt aggtgcagat gattatatac aaaaaccttt ttctgtagat gtcttagtag
     6241 ctaaagttaa tgccctttta agaagaactt ataattttgt tgataataac tcaaatcaga
     6301 ttattcacaa tggtgttact ttagatttat caactgcaac aataaactat gaagataata
     6361 ctattgagct aacaaaaaat gaaatcaaaa ttctccatga gcttatgaaa tataaaggtc
     6421 agatagtaag taggaataaa ctcatgaaaa aattatggga taatgattgg tttgtagatg
     6481 ataatacatt aactgtcaat gtaaatagaa tacgctcaaa attaaatgaa attggtttag
     6541 aagattttat agaaacaaaa agaggattgg gttatataat cagttaaaac aattctaagt
     6601 aggtgaattt atgagcttta aagattttct caaggataaa ctaggttttt tagtatacaa
     6661 ttttactttc ttaattttta cagtatcagt tattatattt tctcctgtag atgtttttct
     6721 gactgacact attttatata tattggttgt aaatgttgtg tttttatttt tatatctatt
     6781 tattagctac atcaagaaga acaaattttt agataagata aaaaatgata cattcaaaat
     6841 taatatgaat gatatagaat tagctagatg taaaaatgaa gaaaaaatat acttgaacat
     6901 tattaaaaat catcaatatc aatgtaataa cataataaat aataatatcg agaaatttga
     6961 agaaaacaaa gagattttaa atatgtgggt gcacgatgta aagataccta tagctataat
     7021 aaaactcatt cttgaacaaa atgaaaattt aattgatgaa aaaattttag atgacataga
     7081 tagtgaaata tttagaattg aaaactccgt tgaaaaaatt ttatatttat caagactcga
     7141 agattttcat aaggattttt taattcaaga ggtaaatatt gaaaaaatca ttagaaatat
     7201 tataaaaaaa tactcaaaat attttatttc aaaaaaaatt attttagact taaataattt
     7261 gaattttaag gttttatctg atgaaaaatg gctatacttc atattcgagc aattattgag
     7321 taattcccta aaatactcct cattaggaga taatatatct atctattgca aaagaaccaa
     7381 aaattattta caattaagag tggaagatac tggttgtggt ataaaaaagg aagatttaaa
     7441 tcgaatattc aataagggat ttacaggtaa taatggaaga aacaattcta aatctacagg
     7501 tcttgggctt tatttagtaa aagaactttg tgataagctt ggtcataaaa ttgatgtaga
     7561 ttcagagtac aaccaatata caaaatttac tattacattc aaatgtgatt tatagtatct
     7621 tattattgat agtttatttt cattaactta tattcaactt cttatagtaa ttctatatgt
     7681 acatcccagt atataattaa gtagttttta taaaaaggag gaagacatgt cttttctaaa
     7741 aaaatttaaa aataaaagat ttagaaataa cagttttaaa agtaacaact ttagaaatag
     7801 cagttttgga agtagtggct tcaagtctaa aaataaaggt tttaaaaaca atccctttaa
     7861 aaataagaga ttcaaaatat atgcaggaat agcagtaatc atacttttaa ttctaggagt
     7921 tcttgcttat gttgattcga aaaacactga attacaagct aatgaaaatt ttatcgaaac
     7981 atatactatt cctgaaaatg aaaaaatatt tataaatggg atggttgttc ctaagcaaac
     8041 taaggacttc aatatatctg gagattatga gttaagtgat gttaatgtta caaatggtca
     8101 aaaagtaaat caaggagacc ttctttttac tgctaaaaat ccaactataa tagcagaaat
     8161 agatagttta aaatcacaac ttagtcaata caaaaaacaa aaaatatcac tttctgatat
     8221 tgctgaaaat aaagatgcta ttgcttctat aaatgcacag ataactgctt taaactctca
     8281 gatagcttct ctggataaaa aagcttatga cagagttact gctccttttg atggcacagt
     8341 atacttgaat aaccaaactg gaaatccaga ccaaccagtt tcatttatga ctattcaagg
     8401 tctagagttt tatatgaaag gacaagcaag tgaacaagat ttacctaaaa tgaagattga
     8461 ccaaatggtc aatatactag ttttttcaac tgaccaaaag cttactggta gaataagttt
     8521 tatatctgat aaaccatcta ctccaaatac tgaaatggga gctcagcaga atacactatc
     8581 ttattatgat ataaacatag catttgataa tcaagaagga ttagtaaatg gattccatct
     8641 tcaagcatca cttgaagtag ctaattcaag ctttaaaata ccagcatctt gtgtacttaa
     8701 agacaacaaa cactcatatg tatttaagga tttagatggt atattgaaaa agcaaattgt
     8761 agatgtagca agtcaaaatg atgattttgc tgtagttaga ggcggtcttg aacaaggcga
     8821 tataataata aaacacccta ctaaagaaat gaaagaagga gacccagttc aaggcgatgg
     8881 cgtaactgct ggttctaata atggagatac tactccaaac aaaaaagtag aagaagttac
     8941 tatggatgtg aattaattta catatgttaa ttaaattaga aaatattcaa aaatattaca
     9001 aagtagggaa agatgaatta catgttttaa aatccttaaa tttagaaatt gaatctggag
     9061 aatttgtcat gataatgggt aagtctggta gtggtaaaac tactttactt aatatcttag
     9121 gttttctaga tgtatttgat gaaggaagat atatatttga tggaacagat gtaactaatt
     9181 tgagtgaaaa tgaacgttct gtttttagaa atattaatat tggatttgtt ttccaacaat
     9241 ttaatttaat agaaactcta aatgtatatc aaaatgtaga gttgcctcta atttacaata
     9301 aagcactaaa aaaatctaat agagaagaaa ttgttaaaga taaattaagt tctgttgggc
     9361 ttttggacaa gcttaagcag aaaccacttc aattatctgg tggtcaacaa cagcgtgtag
     9421 ccattgcacg ttgcctagca aatgaccctc agataatctt tgctgacgaa ccgactgggg
     9481 cattagatag tgaaactagc agagaaatta tggagctttt aacaaggctc aacaaacaag
     9541 gtaagactat aataatggtt acacatgatc aagatttaac taagtatgca actaaggtta
     9601 tacgacttaa agatggtgtg tttacttcgg aggtgtaatc atgagtttat taaaaaattc
     9661 tttggccaat ttaaaaggtc acaagcttcg tgtatttgta gctttactct ggattataat
     9721 aggaataact tcagtaatac ttgttagttc tataggtaat ggatttcaaa aagaaattaa
     9781 gaagtctgta aacaatgtaa accctaataa aactactatt tcgtttgaat cagccgataa
     9841 cacaggtctt actgatgata tgagtatttt tttaaaaccc tttaatgcaa aagatttaga
     9901 agaattatca tttgttgaag gagtagaaag aatagctcct tcaagagatg ggtttaacct
     9961 tgattcagtt tactcttcac aagcatcttt tgataaaaaa actacgtatg ttgatgttgg
    10021 accagtaaaa aaagattcta aaataaattt aatttgtggt agagattttt ctttagatga
    10081 tgaaaaaaga aaagtaattt tacttacatt acaaagtaca agtgaaattt ttgaaaatcc
    10141 agaagatgcc ttaggtcatg gtatcaacat aaatggaact atatttgaaa taataggggt
    10201 attagatgat tctcagcaaa accaagctgg aggattcttt ggaggttacc aagatatgca
    10261 atttactact tctctagtac ctaaaaaggc ttttgacact ttaatgagtc aaaattctta
    10321 ttcaaatgaa atttaccagt tagacttagt ttcttctaaa ggctataatg taaatgaagt
    10381 tgcaaacaat gttatagcta aattatatga gatgcaccct ggtatcaatg gttcttatac
    10441 tacaccagac ccaactgaac agacagctta tcttgaaagc attaattcca atgtaaacaa
    10501 atatgtatct ataataactg tagttgcaat gtttgttggt ggtattggtg taatgaatat
    10561 aatgtatgta tctgttatgg aaagacaaag agaaattggt ataagacgtg ctattggagc
    10621 aaaacctagg tctatattat ttcaattttt agttgaagct gtttttataa cagtatgtgg
    10681 tggaatttta ggaactatag ttggatttgc tgctactaac tatgtttcta aatatatagg
    10741 ttttgaagct atacctagct taaatagctt attttatgca attgtagcta ctatattgac
    10801 tggagttgta tttggtctga ttccagcctt taaagcttct aaactagacc ctataaaggc
    10861 aatttataag tagaaattgt ataaagattt tcgattttta gattagttta tataaaacat
    10921 acttaaatat ataacgcact attatatatg atagattagg aacttaatct ttccaaatat
    10981 aatagtgcgt tctttcataa aaaggattct cttccttact atatctaaat taccaagatg
    11041 ttatgttcta attgttcaaa ctaatacttc caattttgat taatcaatct cattctgcta
    11101 atatacaatc ttttactttc aatcattgta cactccgcct taaaaaaata atcttaaatt
    11161 aggcgttata tacaatctcc ccattacata ttgtataatt aacctttcca tataaagatt
    11221 tacctttaaa tggtgaatta tcagctttag aaacaaagct ttcaactgtc cattcttctt
    11281 ctggattgaa tataacaatg tctgctacag ccccttcttc tataaatcca atatttaaat
    11341 tgtacaactt tgcaggattt atagacattt tttctattaa ctgcatcata ctaagatgat
    11401 ttttatgcac caaatttgtt attccaagag ctaaagaagt ttcaagccca ataattccac
    11461 ttggagcttt tataaactct ctatcttttt cttctttact atgaggtgca tggtccgttg
    11521 ctattatttc aatagtacca tcatttaatc cctctataat cttatctctg tcccatttac
    11581 ttcttagagg tggattcatt tttgcatttg ttccaaactc caatacatct tcctcagtca
    11641 atgtaaagtg atgtggtgaa gcttctgcaa ctacatttgc acctaagtat tttgcaaatc
    11701 taaccatatc aacagataca cctgaactta tatgttgtat atcaacttta gcaccagtct
    11761 ttagtgccaa catacaatct ctggctacca taacatcttc tgctacactt gatgcacctt
    11821 ttaatccaag ttctgaagct acctttcctg catttacacc tggatttcca actaaagaag
    11881 ggtcttcttc atgaaaacta agtggtacat ccaactcttt tgccttaatc attgcctcca
    11941 taattagatt gctatccatt aaaggtaatc catcatcagt aaaccctggt acaccagctt
    12001 ttttaagagc ttccatatca actaactctt taccttcaaa acctttagtt atagctgctg
    12061 cttgtaaaac atttatacat gcattttttg acttttcttt aacataatta aaagtatctt
    12121 cattatctac aataggatta gtatttgcca tacatataac agttgtaaat ccacctctag
    12181 cagcagattt agctccactt tctatatctt ctttatatgt aaacccaggg tctctaaagt
    12241 gtacatgtac atccactaaa cctggagcaa ctacattacc actagcatct attatttttt
    12301 catattcatc actttcatca aacttgccta ttttataaat tttattttct tttataatta
    12361 agtcaatatt ctcatctctt tgacttttag ggtctattac tctaccattt ttaattaaaa
    12421 tcatattatt tctcctttat atttttaatt tatatagact catataatat tatacattat
    12481 tttacatact taaatacaaa attacgtttg acatcaatag cctttttagg acaaagttca
    12541 tggcaacagt aacatcttat acatttactt aagtctataa tagcaccctt agtccctatc
    12601 tctatagtgg atgcaggaca agcttccttg cattttccac attttatgca atcttgaaat
    12661 cttaccacag gctttggtgt tataagcttt gaaataggtt catttaaaaa ttttggtagc
    12721 tttacggaaa acaatcttaa atctttactt acatttggaa tcttgtaatt ttttataatt
    12781 aaatctttta tatcttcacc taaaatttca atatctgaaa aatcttcttg tatatatcct
    12841 ctcttaatag cacctctaat tgtagcaact ctatttggaa gtaaacttat aatcttacat
    12901 gcaacaacat ctatagcata tggattttgt gatgctagta aaactcctat ttctcgagta
    12961 gtaccagctg aaggtccatc accttccata cctactatac catccataat tgtcaatgtt
    13021 ggttttatat aatcacaaat atctaaaagt acattttcgc aaaataactc ttctgtagga
    13081 aatctataat gtatctctgc ttttttcaat ccagctatgc atccatataa gttttttgta
    13141 ccaccagtaa aagttgccat tatatgagtc tttaatttac aaagatttat tatataatct
    13201 gaatctacta ttggtttaat aatatccata tattttagaa catgtgcttt ttcactatgt
    13261 atcttaacat ctgaaacatc ataatttaac ttaatgccca gttcatttgc tacttcttca
    13321 attccagatg ccttataaat gctcttaaga gaactttgat tatatggacc accagggcta
    13381 tctgctattg taatatcaca atttaactct aaaagttttt cacatacaac ttttacaacc
    13441 ataggatgtg tagttgtagc ttcttcaggc ttttttttca tcaataaatt tggttttata
    13501 agtactttgc ttcctttttt aacaaactta tctataccac caatatcctc taaatttttt
    13561 tcaatagata actttacttc ttcataattg taagttttac aactcctaac agataccgac
    13621 ttcatattat ctccatcctt ctatcacaac agtttatttg tataatttaa aaaaggagaa
    13681 actaaagttt ctccttttaa tctatgtttt aattgtttta attattattt tccaaaatgt
    13741 ctgtttaata atcctaagaa tgctttacca tgtctagctt cgtctttaca catttcatgt
    13801 actgtatcat gtatagcatc taatcctaat tctttagctc tcttagctaa ttttaatttt
    13861 ccatcagttg caccatactc agcatcaact ctaactctta agttttcttt tgtatctgca
    13921 actacaactt ctccaagaag ttctgcaaat ttagcagcat gttcagcctc ttcaaaagct
    13981 attctcttat acgcttcagc tacttctgga taaccttctc tatcagcttg tctactcatt
    14041 gctaaataca ttcctacttc tgtacactca ccagtaaagt tagctcttaa tccttcgatt
    14101 atttctgcat ctacaccttg agctactcct attctatgtt catcagccca aaccatttcg
    14161 cctttcattt cttcaaattt atcagctcca actttacata ctggacattg tgctggtgca
    14221 gcatctcctt catgtatata tccacatact gtacaaacaa attttttcat aattaatttc
    14281 ctcccaattt tatataatta aatttttatt tttaagttta ataacttgct acaagaatat
    14341 atatacccca gactttatag cttaaacact aaaaataaaa tttatttcct aattaatttt
    14401 atcactttga aacaccagta tcaaccaagt tttgatgtca aatttctaca taatacataa
    14461 tttatttgat taattaggaa atagttgtca tattaaatac tttctaatga agactcctct
    14521 gaatctttaa gtttcacttt tttgctcttt aaaaaagata atttagtttc tgcagttatg
    14581 attgcaacaa atatcaagat actaccaata accaattttg ctgtaataag ctcctttaga
    14641 attattatag aaaatattgt accaaataca gcctctgtgg ataatattat agctgttcta
    14701 gttccatcaa ctcttttttg acatatagtc tgaaataaaa atccaattgt agtactaaat
    14761 acagctagat acatagttcc catgtaacca gataattcag cttccatctt taattgacct
    14821 gcaaatgttt gaactacaac agacattaaa aacgctactg taaattgaat agctgtcaat
    14881 gccattgggt tgttatcttt tgcaaactca cttgtaaaga atatatgaaa tgcaaatccg
    14941 aatgaacata taagtgttag aaaatctcca aaattaatag agaaatcagc ttctaaagaa
    15001 agtatcccaa ttcctataag agctaccaaa ctacttatta ttcctatttt atcaagtttt
    15061 cttctataaa gtataaatcc tatgaatgga actattacaa cattagctgc tgttataaac
    15121 gcatttttag atggtgtagt gtacataagt ccaatagttt gtactgtgaa tgctagaaat
    15181 aaaaatattc ctaaaatccc tcctgctttt aataattttt tacccatatt ggcacgaatc
    15241 tgtttaaaaa acagtaaatt cataattata gctgccaaga aaaatcttaa agttatgatt
    15301 tgtagaggtg taagcccacc gtctaatgca aattgggttg ctacaaagcc acttccccat
    15361 ataattgcta taaatattag tcctatttct ccaaagtatt ttttcaataa agccccctcc
    15421 tttttatttt tttgcaaaat tataaacccc ctaaacagca agagagtttt tctataagca
    15481 ttactataaa ttaatatagt aaattttttc agtattttaa atctaaattt acatacattt
    15541 tgagctcaaa tttatatcca ctatttaaca gtaatatatt aatcattttt tgtaaacacc
    15601 tttttaataa atttttatat tttttatatt tttttaccta tactaaattt ttttatagat
    15661 atttttaata tatatgatac tttaatatta tgcaaataat gtcatataag ttacacttta
    15721 gttatcttag ttacaacttt gtcggtttac tttttctact ttcttcgata taattataat
    15781 atatttttga tacaattcga caaaaggggg aatgctgtaa ttatggtaaa aaaaagaaaa
    15841 aagaaaactg ttacacttac tatacttgtt gtaatagtta ttggtatttt ttctatggca
    15901 tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc aagaaagact taaagctgaa
    15961 agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac ctattattgg tgcaaaaaaa
    16021 gaagctgtaa aagaatatgg atatgacgca aaaatagttt gggacaaact taacaaatac
    16081 gactattcaa acaatggaga aaagatagtc tttcttacat ttgatgatgg accatctact
    16141 actaatactc cacaagttct agatatcttg aaaagacatg gtgttagagg aactttcttt
    16201 attaagggtg attctcttga aagaaaagga gctaatgaaa ttttaaaaag aacatttgac
    16261 gaaggaaatg caatagccca tcactcttat actcatgact ataaaaaact atatcctaac
    16321 agaagcctaa atctcgatac attcgtaaat gaacttaaca aaactgatga agctatgaaa
    16381 aaagtcttag gaaagaattt ttcctcaaat gtagttagat gcccaggcgg gtacatgtca
    16441 tggaaaaata tggaacctct aggaaactac ttaaaagaga aaaacatggc atcaattgat
    16501 tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa atgcacaaga attatttgaa
    16561 catgcaaaga aatcttctga aggcaaagaa atggttgttc ttttaatgca tgatacttat
    16621 ggaaaacaag aaacagtaaa tgcattggac caaattataa catattttaa agacaatggt
    16681 tatcaattta aaattttaat ataataaaaa agcaagttta ttgaatgtac attttatact
    16741 caatagactt gcttttactt tactctattt tactagctcg atttttacaa attttttctt
    16801 tcctatttga acaactaatt cactctcttt tacaatagtg cttaaatcct ctactttttc
    16861 accatttacc tttactccac cttgagaagc taatcttcta acttcactct tactcttaac
    16921 aatctcatta gaaactagta cttcgattaa gtcaaatcca tcttccttaa cttgaattgt
    16981 ttgtatatct tcaggtattt gacctttttg gaatacagat ttaaatcttt cttcagcttc
    17041 tttagcagat tcttctccat ggtataatgt tactatttct cttgctaagt tcatctttat
    17101 atctcttgga tttactgaac cctctttcaa ctgagattct attttattaa cttcatcagg
    17161 atgaacatct gtcactaaat tgtagtattt aattataagt tcatcaggaa tttccataga
    17221 tttttggtac attattcccg cttcttcatc tattccaata tagttaccta aactcttact
    17281 cattttttct ttaccatcta atccttctat taaaggcatc attattacta tttgagactc
    17341 cataccaaat tctctttgta atgatcttcc cattagtaaa ttgaatcttt ggtcagttcc
    17401 accaagttct atatcagctt ctaatgctat agagtcatat gcttgcatta atgggtagaa
    17461 gaactcatgt actgatattg gcatttgtcc ttcatatctc tttttaaaat cttctctttc
    17521 taacattctc gcaacagtaa tagttgctgc taattttatt acatcttcaa aatttaactt
    17581 agctaaccac tcgctattaa atcttacaat agttttttct ttatctaaaa ctttgaaaat
    17641 ttgttcttca taagtttttg catttgcaag aacttgctct gttgttaaag cttttctagc
    17701 tttagatttt ccagttgggt ctccaatttt tcctgtaaaa tctccaatta ttattactat
    17761 ttgatgacct aaatcttgta attgtttcat ctttctaaga actactgtat gccctaaatg
    17821 tatatctgga gcacttgggt ctagacccaa ttttactatc ataggctttc cttctttttc
    17881 tgatttaata agcttttccc ttaactcttt ctcatctatt aaatcatcta cacctttcat
    17941 aattattcgc atttgctcgt ctatcgattt catttttcat tcctcctaaa atatttatat
    18001 aatctataat ttttattttt ataataaaaa aagctttcat cctcttaaaa ggacgaaagc
    18061 attgcttacg tgttaccacc ttagttcatc aatatctcac gatattaatc tcttagagta
    18121 caattacata tactcgtgta ctataacgtg tactaatccg atatctctta atggttatta
    18181 tctttcagag atagtgctct gagatgtatt caaactaaat caatttattc catttcacca
    18241 aactggaact ctctgttaaa taaaatatag ccctacttct tctcttcata gcatttatat
    18301 attcagatta tttcatcaat tacaagaaat ttttcttaac ttactcataa tatattatta
    18361 tttttatatt ttgtcaacct tttttattat tatatacatg tttttttaca ttatactact
    18421 tttttatatt ttttaccaat acccaaaatt tatttttcaa tcaaaagatt tatttgttta
    18481 ttgactatat tactctgact caatcttata tttattttct tatttaaaaa tgcgtttata
    18541 attattaaac taataactgt tacatcaaat ataaaaaaca gcaataaaat tattatcaaa
    18601 aataaaattc tagcatataa taattttcta aacttagcta ttataaagtt ttgtagtgac
    18661 atacatgagt ctctaatgat aaaatagaca cttatatagg ttattgatat aaagattgtt
    18721 tttaaataaa cagaatcaac tttaacattc aacagtttta gaacttcaaa aatggctaga
    18781 taagaaacac atatgtaaaa agcttttttt gagcaacata aaaattggtt taaatttcta
    18841 aagttcttaa atattaaaaa tttttttaaa gaattagaat ctaaaatatg caatttattc
    18901 cctaaaatta aacttaataa atatatgcaa aacaccatac caaaaggaaa caatgctatc
    18961 attaaataat aaataaaatt tgtataccca ttaaaataaa tccatttgct atatccttga
    19021 aattctttca taaaactata acctattaat gttgaagcat atatatcaat gaaaaccatt
    19081 acaataaccc caaatactga accatatact aaatcatcca taaccattgt aggcttattt
    19141 attagatagc cacacattat accaagtaat acactctgac ttgcccaaat agcagttcct
    19201 agatttccaa gtaccagaga aattattaaa agcgaagtaa ttccagataa aatggtatac
    19261 tttaaatcac atttcaagca gattatacaa aaaaatactg gcactataaa atctaagtaa
    19321 atagcatatc caaacccagt actcacagct ataattgtta ctactatgaa taaagatgac
    19381 aataaagctg cttcagttaa ttttttagta gtattcaaaa cactgcctcc ataaatcata
    19441 aattttctaa ctaaagtttt gtttttaata tagtatatca ctttttaaaa atatttttaa
    19501 taaatataaa ttttaataaa ggtatttatg ttaaaaatgg tataatgtat cttaatattc
    19561 agttttttaa gatgctagga ggtaattact atgtttaaaa ttaaacactt taatgatttg
    19621 agtttagatg aattttatga aatagcaaaa tcaagatatg aggtatttgc ttgtgaacag
    19681 aagatatttt cattaaacga ttatgatgat atagataaaa gctcttatca catattttta
    19741 aaagaaaatg gattgatttg tgcttatgct agaataattc ctaaagaata cagctcttat
    19801 aatgatgttt ccattggtag agttttagtt ctttctagtc atagaagaaa aggattagca
    19861 aaacaaatga tggattgtgc tatagatttt ataaaagtta acttacacga aaataatata
    19921 accttatcag cacaaactta tataaagaac ctttacttat cttgtggttt taaagaaatc
    19981 tcagaagtat acgacgaagc aggtattgaa catattaaaa tgagattata gtgttgacaa
    20041 gctaatattg ttaatatata atttaagtat aaattaaata ttgaattgat atttaggtat
    20101 ttgaaaatac cttacataga gtcagaaggc ttttgctttc tgactttttt attatcaaaa
    20161 ttcaataatt atatggattc aaagtgttaa tttttattct atattctact ttccccaaaa
    20221 gtaagaatta tacccaactt tgtatgatag gcatcttgcc tatctttttt ttgtaaaagt
    20281 aacaaggtaa taatataatg catagtttaa aataagaaga ttagcccagg ctaaatttca
    20341 agcatggagg tgctattatg aaaaacctta acgatattca agtgaaaaaa actgtcaaag
    20401 ttgaagatat attatctagt ggtaatttaa gagaaagaat gttagcgctt ggtcttacta
    20461 gaggagctgt tatagatgta gttagaaaag gtccaaaaaa taatttaact gtctataaaa
    20521 tcagaggtag caagatagct ctacgacaag aagaaagtag cttaatatta gtttctgaca
    20581 tttaaaaatt tctagcagga ggatattaat atggggttga cacataactc aactaaaatg
    20641 agttctttaa aagacatgtt tgatatagac aataaggaag accagtttgt aattgcattg
    20701 gctggcaatc caaatacagg taaaagtact gtatttaatc atcttacagg acttagacag
    20761 catactggaa actggccagg aaaaactgta gctacagctc gtggtaattt taaatataaa
    20821 aataccgaat atgcattaat agatttacca ggaacatatt ctttatttgc cttatcacaa
    20881 gaagaaattg tagctcgtga ttttatatgt tttggtaatc cagatgcagt aatagtagta
    20941 tgtgatgcta catgtcttga aagaaattta aatttagtat ttcaagtgat ggaacttaca
    21001 gataaagtta ttttgtgtat taatttaata gatgaagcta gaaaaaaagg cattactatt
    21061 gataaaaaac ttttagagga ttctcttggt attccagtta ttttaactgc tgcaagaaat
    21121 ggctctggta tggatgaact attggatact ttaaatgatg tatcttttga caaatataaa
    21181 ttaaataata aacctgttag atacaatgaa aatatagaga atgtggtaaa atcaattcaa
    21241 ccagaattgg ataatattat accaggtata aattctaggt ggttgggact tagacttatt
    21301 gatggcgatg aaagtatatt tgaatctatg agtaattata tagacaaaga ttctatagat
    21361 gctattaatg aagtaaagaa aaagattcca gacaatataa ataaacagaa aataagagat
    21421 gaatttacta aaatcaacta tgattatgct aaaaaactga gtgatgagtg ttgttctaat
    21481 gttgctaaaa aatcaactga tagagaagaa aaagtagata aaatattaac atctaaaata
    21541 tttgggcttc caataatgct cttattatta ggtactatat tgtggattac tatagaagga
    21601 gctaactatc catccacttt attatctaat ttattactcg gatttgagcc aagtatttcg
    21661 ggtatattaa atagtattaa ttgtccatca tggttaaatg atatgttggt tttaggatta
    21721 tacagaacat tagcatgggt aatctctgta atgctacctc ctatggcaat attttttcca
    21781 ttatttacct tacttgagga ttttggatat ttgccaagag ttgcatttaa cttagaccac
    21841 ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga ctatgtgtat gggatttgga
    21901 tgtaatgcag ctggagttat tggttgtagg ataattgact ctccaaggga aagacttata
    21961 gctatactta caaacaactt tgttccttgt aatggtcgtt ttcctacttt gatagctata
    22021 tctacaatat tttttagctc agttataacc aactctttcg tatcaagtgt agcaactgca
    22081 ctttgcataa ctttactgat aattctaggt gttataataa cattgttggt ttcttatact
    22141 ctttctaaaa cgctattaaa aggagttccg tctacattta cattagaact tcctccttat
    22201 agagttcctc aaataggaag aactttatat acctctataa tagatagaac aatatttgtt
    22261 ttaggaaggg ctgtaatggt agccatacct gctggtgtca taacttggat atttgcaaat
    22321 atatatatag gagatttaag tatattatca catgttgcaa actttttaga ccctttagct
    22381 aaattaattg gtcttgatgg ttttatatta cttgctttta ttcttggatt cccagcaaat
    22441 gagatagttg tacctatact tttaatggct tacctagcaa ctggttctat gatagaatta
    22501 gatagcttta gtgctttagg tcaagttctt agagaacatg gatggactta tttgacagct
    22561 ttaaatgtta tgctgttcag tttactgcat tggccttgtg caacaacact tttaacaata
    22621 aaaaaagaaa ctggtagttt aaaatggact gccttaggat tcttaatgcc tactatactt
    22681 gcttttgttg tttgtttttt aacaactaca gtttacaatt tatttatata attagtatta
    22741 actgacagta gacatatatt agagaaacta tttaaaaatt tatatacttt attataaagc
    22801 atagagatat attcaatgat aagtaaaatt acttaacatt tgaatatatc tctattttaa
    22861 ttcttatctg gatacttgga ttgaaatttc agtcatataa ttatcttcat tactttcaca
    22921 aaaaaagtca agtatactct cctcatacga atttccaata attttatatt cattttcata
    22981 tatatatttt ttcaattttt cgtaagattt atatgaatcc tcatattttc caatatgttt
    23041 tatgcaagca tacattcctt ctggctttac atatagcttt ttatccttta tttttttatc
    23101 tatcttcaaa taaaaacttg atatacttaa atagtcccca ttttttatat tttcattact
    23161 tactattact cccaactcat atccttgata caaatttttc gatttacaat aattggtaaa
    23221 atctattaat tccatcataa aatcactatt actcatatta ctcaaatcgc ttgatagaac
    23281 taaatattct tcaaactttt tggatatata aatttcttca ctattcattt tagaaattcc
    23341 ttcaactgtt atatttattc tattttgcat cttagaactt atctttctta gtttttttat
    23401 tttttctctt atcttttttt cttcttcttt aagcattttc acacaaagct ggggagttct
    23461 attttgtata tactctttta tttcctctaa actcattcct atttctctta atgattgtaa
    23521 tatatcaagt gtatcatatt gataaactga gtaatatcta taattatttt tatctttata
    23581 ttcaggagaa aaaataccta ttttgtcata gaaaattaat gtctgcttag atataccaca
    23641 caatttagca aattcacctg ttgtaaaata ctgtttttcc aagggttata cactcctttt
    23701 ctttttgttg tctatatagt tactatattc tttattattt actaaatcaa gtagttatta
    23761 ttttagaaaa tcaatatggt tttgatttta aagcttaaat atcagttaga tgtcagttat
    23821 aaatatttta cccattccca tcataaaaaa gatatcccct cttaactttt ggatggaata
    23881 ttatatttat acttgttaga gtgaaaattg taataggtgc tacaataaga tatttaatta
    23941 aacataaagt taattattgt aataaaaaat ctttagataa attagagtta aagtatggaa
    24001 atatagatag agaaaaagct gttaaactag aaatatttta tcaatattta atagaacttg
    24061 aatatataga aatgagttta ttaataagaa gattagatac tgcaataaca tcattagcat
    24121 tagtatctat tataacaata gcatcatatt atttaatcag aaaaaatgtg taattatgta
    24181 agctatttat aataaaacac tgataaaatt aataataaaa agataatagg atttctatac
    24241 tacctttttg tttttataat atagtgtaat tttat
//
LOCUS       DAEMHZ010000049        24268 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000049 DAEMHZ010000000
VERSION     DAEMHZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.049"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..568
                     /locus_tag="KPK72_001886"
     CDS             <1..568
                     /locus_tag="KPK72_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489105.1"
                     /translation="LKFDSIIEYKELLKKIELIDIIDNYNFSREPMNTRNLGKKMINN
                     GNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKFCWIVDGREEYKMKNYI
                     ESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQTIMHVLVSNGILIG
                     EGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            588..1604
                     /gene="cas7i"
                     /locus_tag="KPK72_001887"
     CDS             588..1604
                     /gene="cas7i"
                     /locus_tag="KPK72_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG2489106.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            1620..2417
                     /gene="cas5"
                     /locus_tag="KPK72_001888"
     CDS             1620..2417
                     /gene="cas5"
                     /locus_tag="KPK72_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG2489107.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            2444..4852
                     /locus_tag="KPK72_001889"
     CDS             2444..4852
                     /locus_tag="KPK72_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2489108.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            5293..6240
                     /gene="prmA"
                     /locus_tag="KPK72_001890"
     CDS             5293..6240
                     /gene="prmA"
                     /locus_tag="KPK72_001890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG2489109.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            6259..7017
                     /locus_tag="KPK72_001891"
     CDS             6259..7017
                     /locus_tag="KPK72_001891"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG2489110.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            7019..8317
                     /gene="mtaB"
                     /locus_tag="KPK72_001892"
     CDS             7019..8317
                     /gene="mtaB"
                     /locus_tag="KPK72_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG2489111.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            8360..8710
                     /locus_tag="KPK72_001893"
     CDS             8360..8710
                     /locus_tag="KPK72_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG2489112.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            8873..9052
                     /locus_tag="KPK72_001894"
     CDS             8873..9052
                     /locus_tag="KPK72_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG2489113.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            9082..9525
                     /locus_tag="KPK72_001895"
     CDS             9082..9525
                     /locus_tag="KPK72_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG2489114.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            9636..10133
                     /locus_tag="KPK72_001896"
     CDS             9636..10133
                     /locus_tag="KPK72_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG2489115.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            10326..10889
                     /gene="raiA"
                     /locus_tag="KPK72_001897"
     CDS             10326..10889
                     /gene="raiA"
                     /locus_tag="KPK72_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2489116.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            11102..11338
                     /locus_tag="KPK72_001898"
     CDS             11102..11338
                     /locus_tag="KPK72_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG2489117.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            11351..12694
                     /gene="yqfD"
                     /locus_tag="KPK72_001899"
     CDS             11351..12694
                     /gene="yqfD"
                     /locus_tag="KPK72_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG2489118.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            12633..13748
                     /locus_tag="KPK72_001900"
     CDS             12633..13748
                     /locus_tag="KPK72_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG2489119.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            13805..14266
                     /gene="ybeY"
                     /locus_tag="KPK72_001901"
     CDS             13805..14266
                     /gene="ybeY"
                     /locus_tag="KPK72_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG2489120.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            14272..14991
                     /locus_tag="KPK72_001902"
     CDS             14272..14991
                     /locus_tag="KPK72_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG2489121.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            15110..15511
                     /locus_tag="KPK72_001903"
     CDS             15110..15511
                     /locus_tag="KPK72_001903"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG2489122.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            15575..16468
                     /gene="era"
                     /locus_tag="KPK72_001904"
     CDS             15575..16468
                     /gene="era"
                     /locus_tag="KPK72_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG2489123.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            16480..17859
                     /gene="mgtE"
                     /locus_tag="KPK72_001905"
     CDS             16480..17859
                     /gene="mgtE"
                     /locus_tag="KPK72_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG2489124.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            17932..18087
                     /locus_tag="KPK72_001906"
     CDS             17932..18087
                     /locus_tag="KPK72_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG2489125.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            18053..18826
                     /gene="recO"
                     /locus_tag="KPK72_001907"
     CDS             18053..18826
                     /gene="recO"
                     /locus_tag="KPK72_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG2489126.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            18819..19418
                     /locus_tag="KPK72_001908"
     CDS             18819..19418
                     /locus_tag="KPK72_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG2489127.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            19578..20456
                     /gene="glyQ"
                     /locus_tag="KPK72_001909"
     CDS             19578..20456
                     /gene="glyQ"
                     /locus_tag="KPK72_001909"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG2489128.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            20456..22522
                     /locus_tag="KPK72_001910"
     CDS             20456..22522
                     /locus_tag="KPK72_001910"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG2489129.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(22688..24235)
                     /locus_tag="KPK72_001911"
     CDS             complement(22688..24235)
                     /locus_tag="KPK72_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG2489130.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT         9866 a         2428 c         4408 g         7566 t
ORIGIN      
        1 tctaaaattt gactctatta tagagtataa agaattatta aaaaaaattg aacttataga
       61 tattatagat aattataatt tttcaagaga gccaatgaat actagaaatt tgggtaaaaa
      121 aatgattaat aatgggaatt tatcaaattt aaaagaatta ttacttgata atataagtgt
      181 tttaaggcaa ctaactggaa cttctcgaga aatggatttt gtatctagtt catataatat
      241 cggtttatat ataaaatttt gttggattgt agatggaagg gaggagtata aaatgaaaaa
      301 ctatatagaa agtaatttaa tatattcaag agtaggtaaa gacttattta ataaaatgac
      361 agatgaatcc agaagaaaaa atttttctat gaaagtgatt caactactta agtctaatga
      421 tagaactcag ctatttcaaa ctataatgca tgtgttagtt tctaatggaa tcttaatagg
      481 agaagggttt gtagaaggtc taatgcagag ttctgaatta gaattaaata cgaatgtagg
      541 tctatttatt caagaaatca tgaaataaaa aagggggaaa atttaaaatg aatgaaaaaa
      601 gagtaaaagg agtaacatta actatactga cagaaagccc agttccactt tcgtatgacc
      661 aagggtatgg aaattataca ccaataaaaa aagaacaata tagagataaa gtacatgcaa
      721 aaactagtat agctacaata acatatgatt taagaagaat actccatcaa gaatatggtt
      781 ggaatttaag taatattgtt tttggaaaaa aaggaaatat atatccatct attaagaaaa
      841 atgtaggatg tgtagatgaa aatggattag agactgatgt atttggttat ttaataccac
      901 ttaaagagaa aggttctata agtaaggctt ctccgcttag aataatacct tttaggtcac
      961 taaattcata tagaggttca acacagttga taacaaatag aggtttttta agtagcgaat
     1021 ttggtagaaa atattatgat gagagggaag aaaatgaagt acttagagat gaaaactttc
     1081 caacaacaca agcattagca atagaagaga ctttatcaga ttactacgtt tatacaataa
     1141 cattggagct tgatagaata ggagtagtag aagtagaaga agatggaaag ttacttttac
     1201 cagaagaaag aaaatttatg agtaaggagt taagagaaaa agcagtaaaa gatatattag
     1261 atgcaattac tgtgtttaca agaaacataa aacattcatc agtactatta aaaccacttg
     1321 cagtaatggg tggagctttt gataaagttg taccattttt ttgggatgat gtagattata
     1381 atgctgatag tggtgagata aatttagagg gagtcataga aactatagaa agttatagcc
     1441 ttaaagaaag taacacaata cttgctatca atgacagatt aaaaatttct aataaaaagg
     1501 aattaaataa gtttaattta agtaaatacc ctgtaaaaga gattaaaaat ctagcagata
     1561 gattggaaat tggagaagat aatatgtggt atttaaaaga gtaggaggtt ttttgaatta
     1621 tgaaagtatt acaatgtaaa cttaagcaac caactgctca ctatagagac ccaaaagtat
     1681 ttcaaaatga atatataagt actttaaatt taccatctaa gaccacaata atgggaatga
     1741 taacgtattt atgtgataga agattaaact ctgatataga tataggaata attggaacac
     1801 atcatcatag agaactagaa ttttcaagag gagagaacat tgatttttgg aatgaatata
     1861 caaatatgag aaaaggcaaa gataaagaaa agtttttgct tcaaggaaac tactatgatt
     1921 actataaaga acataaagct caaaatagca tattaaacta tgaagttctt aaagaagtag
     1981 aactgactat atttatttct tgcaaggatg atgaagaact tgaatttatt agaaaaaagc
     2041 ttgaaagtcc atgtaaatat gcaaatttag gaagaaaaga agattttgta ataccaagtg
     2101 aaaaagggtg ttttgtgaaa gaagttgagt taaaagaggt tatacctatg aatactcgtg
     2161 atgctataaa agaaaatata aaattaaaga atacatatgt aagagttgac ttaagagata
     2221 aagagaatgt cgagactata ataaatcaag gtgtattgat tgcattacct tataaatata
     2281 aagatttaga agcaaataga gatgatagag tttatgagtt ttgccattat atatatgttg
     2341 ataatgatgg tatatatcct aaaaatatta aggtaaatgt atgtattgat acaaaagaag
     2401 tttttacatg gctttaaatt attttgaaag ataggtgttt atcatgaaaa atatcatgca
     2461 aaaagaaata ttagctaagt catataattt aagaactaat caacaagagc aaacattagt
     2521 tgaacatatt aaggatttat ttgaagtatt agaatctata ttagaactaa atctatattc
     2581 agataaagat gtagagatac taaaaatatg ttgtgctttg cacgatttag gaaaaattaa
     2641 ttcaattatg caacaaaaaa tggagattaa taataaaata tcgtatagtt gctcagaaga
     2701 agagaggaaa aagttagagt ctgacaaaaa atctttaaaa aagatagcaa gacataatat
     2761 attttctgga gcatttttaa aagatatatt agaaaaaatg aacttgtcag aagaagataa
     2821 gttatatata tataaaagta taatgttaca tcatggaaac tatgaagatt atatgaggtt
     2881 aagtattagt aaagttcaag aagaaattta tgagtatatt gaaaaaggca ttttagaaaa
     2941 agaagagttt aatctgaaag atatagaaag ttatataaat agcattttaa atgtagactt
     3001 taaatttggt gaagatgtat tggattatga ttacatagat aaattaagtg aaagtatgtt
     3061 tatagacagt gattataaca atggtcaaat agatgattca gttttaaatt atagaaagtt
     3121 taagtatatt ttatataaag ggatgctgaa cttaatagac catagtgcat caagtagaca
     3181 gaaaggtata aaattttata acgattttac tgatgaagaa attgataata tgattttaaa
     3241 tgagatatat aagagtcagg gtaatttgga aaaaaataat atagagttca atactataca
     3301 aaaaaggttg agagaacttt ctggaagaaa tgttctgact gttgcattta caggttcagg
     3361 aaaaacagct gcagattata gaaagacttt taaaagaaaa ttctttttag tgccaaataa
     3421 aatatcagca gaaagttttt atagaaaaaa tatttttcaa aataaaaatg atttagatac
     3481 aagaagtagt aatgattaca ttggattact tcatggagat ataaacttat attcagagaa
     3541 tgaagataat ggagaacatg attttgtatt gacattaagg gatatagatt tgagtataaa
     3601 cttttgtaaa ccttgtgtaa ttgcaacagt tgaccagtta ttacttagta tgtttaaatt
     3661 tccgggatat gaaaaaatat tcgcagccgt aaaaaatgca tctataactg ttgatgaagt
     3721 acatttattg gaaccaaaga tgtttttgat tttgatatat tttatgcagt ttgcatgcaa
     3781 gtacttagat gttgattttc acttaatgac agctacactt ccaaaatcat ataaagagca
     3841 aatgataaac aagggaatta tttttcaaga agaaagtaat gaaaatgtta ctgataaagg
     3901 agaaatagtt tttattgaga gtaacaagga agaagagatt tgtgaaggta aagatgtaaa
     3961 ggtttctttt ataaaggaaa aagaaatcaa gtcaatagtt gaagatgctt tagaaaataa
     4021 acaaaaaatt cttataataa agaatactat tgattcagtc aatagaacat atgagttttt
     4081 aaaagaaaat ttatctgata agtatggtgg tgtagatata gatgttttac atagtagatt
     4141 taagttcaaa gataaaaaag agaaatatag taaaatatta aatggagagg gtgatatttg
     4201 gatatcaact caatcagtag aaatatcctt ggatttggat ttcaatataa taataagtga
     4261 tttagcaact atggatagct tgatacaaag aatgggtaga tgtaatagaa ataataaata
     4321 tgaatatgga aacttttata ttctacctag tgaagataaa atttatgata acaaacttaa
     4381 gaacacaact aaaaacatct taaaggacat attaaaaact aactctattt ttacaatggg
     4441 aattagaaaa actattttgg atgattacta tgataatagt gtagtcaaaa aatattttga
     4501 agagaatttt ataagttgtg ataaagaaat taagaatatt tatggtataa ataaagaaat
     4561 ttttgatgga ttggatttaa tttttaattt tgaaccttac aaaaatattg ttgatagtaa
     4621 gaaggaagca gctaaaattt ttagagatgt tgatgttagt tacaaaatta ttctggaaga
     4681 agatttttat aaggaagata gggagttaca acaagattcg attcaagtat cagggtttat
     4741 atttaataga ttatattatt atggattaat aagcaaagta gaaggataca tggtactaaa
     4801 atctagtagt aagtttgagt ataatagtac tgtaggctta attttgaagt gagcatatgt
     4861 ttaaatataa tataccttta gacattgaaa atacaaaaag tactactgta agtccactgt
     4921 aaaaactatt ttttaaaagg aaaaataagt tgtgagatag agtgtttaca agtgtatata
     4981 gataataaat ttgaggttta agattaacta agtgggtttc aggtacatcg cgaaaataat
     5041 tataagttgg agattttgta tgtttaagat taactaagtg gatttcaaat attgatatag
     5101 tagatataat agaagtttta ttattaggtt taaaattaac taatcggatt tttaaatatt
     5161 ctgactatta tataaggata ttattagctt gaatttaaaa ccaataatat aaaacaacaa
     5221 aatattatat ttatgatata ataaaagtta tggaaaaaca ggaatattac tatagagaag
     5281 agaggtaaac ttatgaataa ttggatagaa gtaactataa aaacaacaac agaagcagtt
     5341 gaacctataa cgaatatact atatgaacaa ggagctggag gagctgtaat agaagaccca
     5401 aaagactttt tattccaaaa gaaaaatgaa ctagactggg attatgttga agaagaagta
     5461 tttaaaaaga atgaagaaga tgatgttctt ataaaaactt atgtttctga agaaaaaaat
     5521 gtgatggaat ttgtcgaaat aataaagcaa aaagttttag gattaaaaga ttttggtata
     5581 gatataggtg aaggaagtgt atcattggac caagtaaatg aagctgattg ggcaaatgca
     5641 tggaaagcat actacaaacc aacaaaagta ggacaaaggg tagtagtaaa gcctacatgg
     5701 gaagattatg ctatgcaaga gggggattta atcatagagc ttgacccagg aatggcattt
     5761 ggtacaggga cacatgagac tactagtatg tgcattagag aattagaaaa gtatgtgaat
     5821 aaagattcta aagtatttga tattggatgt ggtagtggaa tccttgcaat agcagcagct
     5881 aaattaggag caaaagaagt tgtagctgta gatttagatg aagtggctgt aaaagttgca
     5941 aaagaaaatg ttcttgaaaa taaagttgaa aaaagtgtat ctgtaatgca tggtaattta
     6001 actgatgtaa taaaagataa agcggatgta atagttgcaa atataatagc tgatataata
     6061 aagatacttg caaaagatgt tcaaaacttt atgaaagaag atgctatatt catatcttca
     6121 ggtataattt tggataaagt agaagaagtt aaagaaagct taattgaaaa tggatttgaa
     6181 atagttgaag ttcaaaaatt aggtgaatgg tcagcaatag tttctaagct taaaaaataa
     6241 gggatatggt gacgtataat ggatagattt tttgttgaaa aaaataatat aaatctacaa
     6301 gataaaactt gcactataga aggtgaagat gtaaaacata tttcaaaagt tttaagatgc
     6361 aaattaggtg agaaacttga aatatgtgac aagaataata atgagtatat ttgtgagata
     6421 atgaatatag ataagagtat agttaattta gaaattttag aaaaagttga tataaataga
     6481 gaatcagaac ttaaagtaag gctataccag ggacttccaa aagctccaaa aatggaaatg
     6541 atacttcaaa aattaactga agtgggtgta gaagaaataa tattagttca aactaagaga
     6601 agtgttgtta aagttgatga caaaaaagaa gataaaaaat ttgaacgttg ggaaagaata
     6661 atttatgaag cagcaaagca aagcaaaaga ggtaagatac caaaattaag aggtgtttta
     6721 agttttaaag aagctttaga agatatgaaa aaaaataatg taaatatttg cccttatgaa
     6781 aatgaaagaa cagtttcaat aaaacatgca ctaaaaaaat gtgattctaa tatagatagt
     6841 gttggaattt ttataggtcc agaaggtgga ttttctgaag aagaaataga acaaatacaa
     6901 aaaaataatt gtaatgttgt atcattgggg cctagaatat taagaactga gacagcttct
     6961 gttgtagcat caacaatagc tttgtatgaa ctaagtgatt taggaggaga aaaataaatt
     7021 gaaaaaagta gctttttata cacttgggtg taaagttaat caatatgaaa cagaagctat
     7081 gctagaacta tttgagaaag atggttatga acaagtcaat agtgaagagt atgctgatgt
     7141 ttatgttata aatacatgta cagttactca tatgagtgat agaaaatctc gtcaatatat
     7201 aagacgagtt aaaaagaaga atccagatgc aataatagct gttgtaggat gttattctca
     7261 ggtatctccg gaagaaattt tggatataga agaagtaaat ttagtcatgg gtacaaatga
     7321 tagaagaaag attgttgaag aaattaaaaa gataaattca agtaagaaag ttagtacagt
     7381 tgatgacata atgaaagtta aagcttttga agaaatagaa atcagccaaa ctaatggaaa
     7441 gactagagca tttatgaaaa tacaagatgg atgtgacaga ttttgtactt attgtatcat
     7501 tccttatgcc cgaggacgcg ttagaagtag agatattgat agtatagtcg atgaagttaa
     7561 aaaattggct aataatggct ataaagaggt tgtattaaca ggaatacatg ttgcctctta
     7621 tggaaaagat ttaaaggata gggatattaa acttcttgat gtaataaaac aaataaacca
     7681 gatagaaaaa atagaaagaa ttaggcttag ttctgttgaa ccaattttat ttacagatga
     7741 atttgtaaat gaagttttaa agatggataa agtttgtcct cattaccatt tatcacttca
     7801 aagtggatgt gatgaaactc taaaaagaat gaatagacga tacacaactt tagagtacaa
     7861 aactattgta gatagattga gaagtaaaat gccagatgtg gcaataacta cagatgtaat
     7921 agtaggtttt cctggagaga caaatgaaga gtttaaaaaa acgtacgagt ttttaaaaga
     7981 aatagagctt tcacaaatgc atatttttaa atattcacca agaaaaggaa ctccagctgc
     8041 aactatggaa aatcaagttg accctcaaat gaaacacttt agaagtgaac aacttttaaa
     8101 tttaagtaaa gtaaatttta ataaatttgc aactaaattt atcggccgtg agttggatgt
     8161 tttatttgaa caaaatatag aaggcaataa atatgaggga ttaacttcaa attatataag
     8221 agttgttgtt gaaagtgaca aaaatataca aggtcagata ttaaaagtga aaataaatga
     8281 tgtaaaagat gaatatgtag aaggaatttt gctataacat gtagaaatta taatatttat
     8341 aagataggag gtggaaagaa tggattgtat tttttgtaaa atagcgaacg gagaaatacc
     8401 ttcaactaaa gtttatgaag atgatagagt cttagctttt aatgacttga acccagttgc
     8461 tccatatcat atattagttg tacctaaaaa acattatgat agtttaattg atattcctga
     8521 taaagagatg gacattgtgt cacatattca tgttgttatt aataaaatag ctaaagagaa
     8581 aggttttgac caaactggat tcagagtcat aaataattgt ggaagtgatg gagggcaaga
     8641 agttaaacat ttgcactatc atattttagc tggtaaaaag cttcctaatt acgaagcggg
     8701 acaaaactaa actaaattat tgaaaaattg agttccttct tgataaaaat acttgataaa
     8761 agtaaaaatt tagtttataa taaacaaagt gttaggttgt aatgcatatt atgcaaaaag
     8821 aacatagtta cagtcccagc ataattgcgc tattggaggg agggaaaaag aaatgtcaga
     8881 agtaagagtt agagagaatg aaacattaga cagcgcttta agaagattca agcgtcaatg
     8941 tgcaatgtct ggcatcatgt cagaagtgag aaaaagagag cactatgata agccaagcgt
     9001 taagcgtaag aaaaaagctg aagcagcaag aagaaaaaac gctaagaaat agtagtaaat
     9061 aagaataaag aggtgaaggg gatgtccctt aagcaaaagt tacaagagga tttgaaatct
     9121 tcgatgaaaa acaaagatac agtgagaaaa tctgtagtaa ctttaataag agcttctata
     9181 aagcagtatg aggttgataa tagggtcgag cttgatgaag atggaattat cgatgtaata
     9241 gcaaaacaat taaagcaacg tagagatgca ttagttgaat ttgaaaaagc tggaagagaa
     9301 gacttaataa aagaaacaga aggtgaaatt gaagttttaa aagagtacct acctcaacaa
     9361 ttaagcgaag aagagttaga agaaattgta aaatctacta tatctgaagt aggggcaact
     9421 tctatgaagg atatgggaaa aataatgtca gttatccaac caaaagttaa aggaagagct
     9481 gacggaaaac ttataaataa gttggtaaaa caaaacttac aatagattga tatagcctag
     9541 agagaaatct ctaggctttt tatagttttt gggaataacg agtcatattt gagcatatct
     9601 ataatcaaag aaaattttga aagaagggga aaaatttgat tatatcagta aaagagatta
     9661 ggaatttttt gataaacata tttataccac taataatagg ttacttaagt agcatcttaa
     9721 caatgattat cagtggaaca gatatttcaa cctattattt acaactcaca aagccaagct
     9781 ttgcaccacc agcttttatc tttcctatag tttggactat attgtacata ttgatgggta
     9841 tatcttcata cctaatatta agaaagggtt acaacttacc taaagtaaga gatgcaattt
     9901 tttattatgg attacaactt gtattaaact ttatttggag tatactattt tttggatttg
     9961 gacttagatt tacagctttg atagatataa taataatgat tttggtttct ataataatga
    10021 tttcgaagtt ttctaaaata gataagagat ctggatacat aaatttgatt tatttaatgt
    10081 ggcttgttta tgcaggattt ttgaactatt ttatatggtt tattaataaa tagaatgtaa
    10141 agattttgta aattaccctg tacttttaat aatatcataa caaacgaaaa ataaatagac
    10201 tattaaaaat aatcattttc atatacaata atattaattt aaatacaata aatttataaa
    10261 aattgtattt aaagattaac atattaaagt attagaattg gaaaggtttg aagggagagg
    10321 tttttatgca aataatagta tctgggagac aaatgaaatt aactgacgga ataaaaggat
    10381 acgtagatgg aaaattaagt aggttagaaa aataccttga tcctgaatca gaagtgaaag
    10441 taacagtaag tgcgaaaaag gatagacaaa aagtggaggt tacaataatt cctattaacg
    10501 gtcaaataat aagagcagaa gatgtagaag atgacttata tgcagcaatt gatattgttt
    10561 gtgacaaatt aagtagacaa gttgtcaaat ataaaactaa agttaaagac aaagttcaaa
    10621 ataataagag cataagattt gaaaatcttg actttataga taatagcagt gaatttgatg
    10681 attatgatta tgatgatgaa gaagatgaaa atatagtaat agaaagaaga aagaaattta
    10741 atgtaaaacc tatgagttct gaggaggcga tacttcaaat ggaactagta ggacacaatt
    10801 tctatatgtt tagaaaccaa gataattttg aaataaacat agtgtataag cgtaaagctg
    10861 gtggatatgg tttaatagaa caagattaat aaaaattgta tacgtttaga ttttatataa
    10921 gaaaaaataa aaaggtaatc atgttgattt tttatcaata tgattacctt tttcttgttt
    10981 aaataatata ttagatatta ggtatttatt atataaaaat tttaaaacta atctgttaca
    11041 acatcaataa agtgcataaa tacctatttc aagcataaaa ttaattaagg gggtaatgct
    11101 tatgttagaa atatccactg atttacaagt aaatcaacca gttattactg ttacaagtaa
    11161 tacgtttatt agtatagaaa attatctatc aatattagaa tatgaagttg atttaataag
    11221 aataaagact aaagttaaaa caattaaaat atctggggat aaactttcac ttaagtatat
    11281 tactgatagt gagataggta taaaagggat tatctacaat gttgagtatg ttgattagga
    11341 ggagatacac ttgataagtt ttataagggg atactatgtt atagttgtgg aaggagtagg
    11401 actcgaacaa tttttaaatc atcttataag aaatggaata agtgtttaca atgtaactag
    11461 aataaaaaat actaaaatgg agtttcatat agatagacaa gatataaaag aatttaagaa
    11521 tgtttataga ggaagtaagt ttgatataaa ggtaaagcaa aaaactgggg ttccttttat
    11581 tatcaaaaga gtttataagc acaaaggtat gtggatttgt gctcttgttt ctttattttt
    11641 attgatgtct acatcacagt ttgtgacaga tgtatatata caatctcctg aaggtataaa
    11701 aaaagaagct cttagaaatg agctttataa ggttggagta agaccaggag tctataagaa
    11761 gagtattgat agaaaagaag ttagagacca tatgatgtca aaatttaatg atgtggcata
    11821 tttatctatc aatgtaaaag gaacaaatat atttgttact gttacaaaaa aggcagaatc
    11881 cctaaaatct acagaccaat caaattattg taatgtaatt gctttaaaaa atggtataat
    11941 agaaaaggta atccctagaa gtggtaaatc agttattaaa tctggagaca tagtccaaaa
    12001 gggagatgtc ttattaaatg gagctaatac caagtcaata ccagaagtat gggcaagtac
    12061 tttttatgag tccacaaaaa aggcaagtta tgtagatact gtaaataaaa aaactggaga
    12121 aaagaaaaat atatatacac taagttttta tgataaagag tttacaatga gaaaaaatat
    12181 aaaatataaa gattatgttg ttgaaaataa agaaaaaaaa ctatcaatag gaaactatac
    12241 ttttccaata aagataaaaa cgagtacttt ttatgaaaca aaaaaggttg aagtaaaaag
    12301 gaataaagaa gagttaaaaa aagaattatc tgaaaaagct ttaaaagagt tagaatacat
    12361 aatcccagct tcggctagaa taatagatgt aaaacataac tttaaggtta ataaaaatat
    12421 gttagaatat ttaataacag ttcaaactag tgaaaatatt gctaaaattt atcctcttag
    12481 taagtcagaa gcagaaaggt ttataaagga agaaagtaaa ccagatgaag gtgaagagga
    12541 agttccttcc aatccagaaa aaagaccatt aaatgatata agaaatgagt ttgatgagga
    12601 taataaggat aataaagacc aaaacaattc tgatgaaaat aatagtaatc aaaatagtaa
    12661 taatagtcaa aataataata gcaataacaa ctaataaaat taaacttaaa atcttaggag
    12721 gactaattca attgataata caaaagaaat ttacaatagc agatggtaac tttgaaagag
    12781 agttatttgg caactttgat gaaaatgtaa aattaataga gaagacttta aatatagatg
    12841 taatacttag agaaggcaat ataattttaa ttggagaaga aaaaaatgta gattctgcat
    12901 taaaacttat gaatgaatta caccaaactg tatcaaatgg taagcattta gataagcaga
    12961 gtatttctta ttcattatct cttttgcttg aaggaagtga acaaaaaata aaagaacttg
    13021 aggggacaat cgtcataact caaaggggaa aagctgttca acctaaaact ttaggtcaaa
    13081 aagaatatat aaaacttata gaaaataatg atataacttt tggtgttgga cctgctggaa
    13141 caggaaagac ctatcttgcg gttgctatgg cagtaaaagc ttttaaaaga gatgaagtta
    13201 gtagaattat acttacaaga ccggctgtgg aagcaggaga gagtctaggt tttttaccag
    13261 gagatttaaa ggacaaagtt gacccatatt taagaccact atatgatgcc ttatttgaaa
    13321 tgttgggtgc tgataaattt aataagtatt tagaaagagg aactattgag gttgcaccac
    13381 ttgcatttat gagaggtaga acacttgata attcatttat aatattggat gaagctcaaa
    13441 atactacttc tgaacaaatg aaaatgtttt taactagact tggatttgga tctaaagctg
    13501 tagttacagg agatgtaact caaacagatt taccacaaaa taagaagagt ggtctaatac
    13561 aagctacaga gattcttaaa ggtgttccag gtattgggtc tataatgcta acagatagag
    13621 atgttgtaag acatgagttg gtacagagaa taataagagc ttatgagaag cacgacaaaa
    13681 gagaagaatt taaaaaagaa gaacgtaaaa aagcgaaaat gcaggaaaag actttcaaaa
    13741 gaaagtaaaa gtgtagtaat atagatatat agcattatta atgaataaaa agtaggtgta
    13801 gtgaatggat ttgatactag atgatagaca agataaatta gaagtaagtg aggagttaat
    13861 tgaaaagata aaggatataa tcatagaatg tttagattat gaaggatatg atgataatta
    13921 tgaagtgagt ctttcttttg tagataataa agagattcat gaattaaata gagaatatag
    13981 aggagttgat agagtgactg atgttttatc atttcctctt ttaagtgatg attttgaaga
    14041 tgttgagtta gaagaagaat ccctaggtga catagtagta tctcttgaaa gagccttaga
    14101 acagagtata gagtataacc atagttttga aagagaagta tgttttttaa tatgtcatag
    14161 tatgtttcat cttttaggtt atgaccacga tacagatgaa aatacaaaag aaatgagaga
    14221 aaaagaagaa cacatattaa ataagttaaa cataacaagg gagtagtttt tatgaaacca
    14281 gaaaaaactc gtcaaggtat aattaaagca tttaatgcag ctatagaagg gatattgtat
    14341 acatttaaat ttgaaagaaa tatgaaaata cattatttag gttcagttgc tgttcttata
    14401 ataagtttgt tttttaactt ttcaaaatta gaaatgataa tgttacttat gtccatatgt
    14461 ttagttgtag ttgctgaaat gtttaataca gctatagaaa aagctgttga tttagtaact
    14521 gatgaatatc atgttcttgc taagatagca aaagatgttg cagctggagg cgttctagtg
    14581 gcagcactaa attcagtagt agttggatat attttgtttt atgataaatt gacagatata
    14641 tctggaatat taatttataa aataagagaa tcagaattac atataacctt gatatgtata
    14701 ttattggttc ttatagctgt tgtagttgta aaagcattga cttctacagg tactcctcta
    14761 aaaggaggaa tgccaagtgg tcatgctgcc ttagcatttg ctatagcaac tgcaattaca
    14821 cttatgactg aaagagttgt tgcctctact cttgcatata taatggctgt attagttgcc
    14881 caaagtagaa ttgaaggaaa aatacataca ttttgggaga ctattgctgg agctttgtta
    14941 ggtgtgttga tagctatatt agtatttcag cttggaatgt tctataatta aaataaatgt
    15001 acataaaata tttaaaataa aacaaatctg tgctaattaa atttttatga tataatgtag
    15061 agtattaaat aaaaataact tttaattatt gtatacagga ggagttgttg tggataacat
    15121 agaattgtta aggttggcag aagatgccag acaacattct tatgctcctt actcaggttt
    15181 tagagtaggt gcagctcttt taactaagag tggtaaagtt tatactggat gtaatataga
    15241 atgtgcatca ttaggtggaa caaattgtgc agaaagaaca gctattttta aagcaatatc
    15301 agagggtgat aaggatatct ataaaatagc tatagcaagt gataactcag aaaataatga
    15361 gcaaacatat ccatgtggaa tatgcaggca ggtaattata gagtttggtt ctgatataaa
    15421 aataataacg ggatatacta aaggagaggt gtttgagcat agtataaagg atttgttgcc
    15481 aaactatttc tctggaaatg atttaaaata aatttaaaag tataaataaa tttaagtaat
    15541 taaaacaaga agataaaaag ttaggagaca gttaatgttc aaatcaggtt ttgtcagtat
    15601 agtgggtaga ccaaatgtag gaaaatctac tttaatgaat aatgtagttg gagaaaaaat
    15661 agctataatg agtgataagc ctcaaactac aagaaataca atacaagcag tatatacaga
    15721 tgaagaaatg cagatagtat tcttagatac accaggtatt cacaaaccaa aaaataaatt
    15781 aggtgaattt atggtaaagg cagcaacaga agcttttaaa aatgtagatt taatattatt
    15841 tgtagttgat gattctaaaa aaataggtcc tggagataga aagataatag aggatttaag
    15901 aagtgttaag acaccaataa ttttagttgt aaacaaaata gaccaactag accaaaaaga
    15961 tgagctattt gatataatca aaatgtatga tagagaaggt atttttaaag aaatagtacc
    16021 aatatcagca ttaaaaggaa agaatacgga taccttaata aaagttatac aaaattattt
    16081 agaagaaggg ccaaagtatt ttccagatta tatgattaca gaccagccag aaagagtatt
    16141 aatagcagaa cttataagag aaaaagttct tcactatctt aatgatgaaa tacctcatgg
    16201 tgtagctgtt gaaattgaaa aaatgaaagc gagaaatgat aaggaaatag tagatgtatc
    16261 tgctgtaata tactgtgaaa gagattcaca taagggaata ataataggaa aaaatggtag
    16321 gaaactaaaa ggaataggta agtcagcaag acaagatata gaattgcttt taggttcaca
    16381 aataaatctt caattatggg ttaaagtcaa agaaaattgg agaaatttac aaaattatat
    16441 aaataatttt ggatataatg ataaataaag gtgggttata tggataggaa actggatgct
    16501 tcccaagatt tattatatga agtaaaatca ttaattgata ataataaagt attggagtta
    16561 agagaattaa tagaggaata tcatataatt gatatattcg atataatgga aaatttagag
    16621 gaagacatga aaattcaatt atttgaagta cttcctttag atatggcatc ttctatatta
    16681 gaagaaggta gtgtagagtt ttttattagt attttatcta aactggatgt agaacactcg
    16741 aaaaatatac ttgaacttat gtctcttgga gatatggcag acaagttgag tgaacttgaa
    16801 gaagaagaaa gagaacacat aataaatctt ttgaatcaag agaatgccga ttatgtaaaa
    16861 gagttactat tctatgatga agattctgct ggtggaacaa tgaccactgg atatatttct
    16921 ataaataaag atatgacagc tcttgaggct atagaccata tgagagaaga agctgaagaa
    16981 gctgaaacta tttattatat atatgtggtt gatgatgaag aaaaactagt tggagtattg
    17041 tcattaagag aattaataat agctagagac gcaaatatag tagaagattt gatgagtgaa
    17101 aatataatct ctgtttatgt agatgaagat agagaagaag cagtaagatt agtatctaaa
    17161 tacaatttaa tagcaattcc agtagtagac agacaagaaa aattaaaagg aataataaca
    17221 gtagatgata taattgatgt aatggaagaa gaagcaactg aagatatgta taaatttgca
    17281 ggaagttcag aacatgaaag agaagttgcg gaaaaagaaa accctacatt gagagagcaa
    17341 ataatatcag cacttagagg acgattacct tggttaatta ttaccttagt tggagggttg
    17401 ttggcttcgt taatactttc taacttagac tatataatga atcctgtata tgctccatta
    17461 gtatttttta tacctgtagt aattggtatg ggtggaaata ttggaactca atcatcatct
    17521 gttacagtaa taacattgtc taataaggat ttaaatttta gcaatgtggt tagagaaggt
    17581 attgtaggta ttattacagg tcttttgtgt agtataataa caggtattgt aatttacttt
    17641 gttatgagaa agttagacat tgtattaatt gtttcaatat cattatttat aaatatggtt
    17701 ttaggtgcaa caataggtgc gtttatgcct gttttattaa aaaaaatgga tgcagaccca
    17761 tctacagttt catcaccaat tatatctaca gctcttgata taactggtat agcagtttac
    17821 tttataataa caacggcatt attgtcaaaa attgtttaat aactttatgt atgatttatc
    17881 ctcttcttac taaaaatata agtataaatg taagaagggg gttttttaaa aatgaacaat
    17941 atcagttggg aagtttttaa aaaaacaggt agtatagatg catatttata tttctgtgat
    18001 tgcaaaaaca tacatgaaga aattgaagaa gtaagggaga aaaaagaaga cgatgataat
    18061 cctgaacacc caggggatag tacttaaagc tataaggtat aaggaaagtg atataatact
    18121 tacactattt acaagaaagc ttggaaaagt atctgcaata gcaaaaggag ctaaaaaaaa
    18181 taagagctct ttgctttcat catcacagtt attttcttat agcaatttta ccttaaaaaa
    18241 acaaggtaat atgtacaagg ttactcaaag tgaaataata aaaagttttt ataatatatc
    18301 ctatgatata gaggcatttt cttatgctac atatattaca aaattagttg aaaattctat
    18361 acttgaaaat cagactaata ataggctatt tatattactt gcgcagactc tgtatttgta
    18421 tacacaggat aatacagaca ataggtttat aacagcagca ttcgagttaa aatttttaga
    18481 ctatatagga tttaaaccta tagtaaataa atgcattaat tgtgaatgta aaattttaca
    18541 aaattctgtg tttaatatct acgaaggtgg tatactatgt agtaagtgta gtaaactttt
    18601 tgataataat ataaaattag acttaacaac tataagtctt atggaatatg tattaagaaa
    18661 tgatatttta acatgtagta aagctaaagt atcaaaatat ataacacatg aacttgagaa
    18721 tattctagac aagtatttga aagtttatgt tgataatatt aattttaaat cattacacgt
    18781 attgcaaagt gtaaaaaata ataagggagt ggataatgat gagtagtaat ataactattg
    18841 aaatggttga ttcagtcttt gaaagagttc caggagcaac ttatgcagaa gcaaaagaag
    18901 cattagtaat gtgcaatgga gatgtaattg aggcagtaat atatttagaa aataaaaaaa
    18961 atacttgtaa ttgtaaagca aaatctgcta aagaaactat ggaagaagta tttggcaaag
    19021 atggagaaaa tataaagttt caattaaaag agcttcttag aaaaagtagt gtagtgcgaa
    19081 taatagttga aaaagatgga aaaactatga tgaacattcc tttaacagtt ggagttgttg
    19141 gattagcttt tgtaccatta gctactttag ttggattatc agctgctgtt ataagtaaat
    19201 atcgtataaa agttcaaaat gaagaagatg gagaaattgt agatttaggt gaattgaatg
    19261 aagaaaaact tcacgttttg aagaatatga taactaatgc agcaaaagat gtgaaggatg
    19321 tagtagttga caaaaaagaa gatgacaaag atgtaactgc tgaccttatg aaagaagaat
    19381 ctgaaaagaa taaagaagac gaagacgaaa ataaatagat ataaagtgac ttttaaaaaa
    19441 tgaagagaag ataattataa gaattatcac taacattgac aaattttata gaatttgata
    19501 ttctaaaagt tgtagaagaa ctattaaaat agcctttcgt gtagacgaga ggctttattg
    19561 ttataggagg gatttttatg aattttcaag agatgatact ggcgttgcaa aaatactggt
    19621 caaagcaagg ttgtatcatg atgcagcctt atgatattga aaaaggtgca ggaactatga
    19681 atcctaatac ttttttaaga tcattaggac ctgagccttg gcaagtatgc tatgtagagc
    19741 cttcaagaag accagcagat ggaagatatg gtgaaaaccc aaacagacta tatcaacatc
    19801 atcaattcca agttatacta aaaccttctc cagataatat acaagaatta tatttggaaa
    19861 gtttaaaaga gattggaatt gacccaagtg agcatgacat aagatttgtt gaagataact
    19921 gggaagctgc tacagttggt gcttggggac ttggatggga agtttggcta gatggtatgg
    19981 aaataactca atttacgtat tttcaacaag taggtaatat tgaatgtgag cttgagacag
    20041 gagaaattac atatggatta gaaagattag ctatgtacat tcaagaagtt gatagtgttt
    20101 atgatttaaa atggaatgat aaaataactt atggtgaagt atttaataaa gctgagtatg
    20161 aaaactcaat gtacgcattt gaattatgtg atgcagatat gttgtttaat ctgtttgata
    20221 tatatgaaaa agaagcatta agacttatgg aaaatggcct tgtaatacct tcatatgact
    20281 atgtgttaaa atgctctcat gcatttaata cattggatgc aagaggtgca ataggagtta
    20341 gtcaaagagc ttcatttata ggtagagtta gaaatatggc aaagactgtt gcagaaactt
    20401 ttgtaaaaca aagagaagaa atgggattcc ctcttttaaa ggacggtgac aaataatgaa
    20461 aaattatctt ttatttgaaa tcggtgttga agaaatgcca tcaagatttg taggcagtac
    20521 actagaacaa ttaaaaaaca atcttagtaa attatttgat gaaaatagaa tagcttttga
    20581 taaaataaat gcgtatggga caccaagaag acttacttta gtagtcgaag gattgagtga
    20641 tagacaaaat gatttagaag aggaaataaa gggtcctgct aagaaaattg ctgttgatgc
    20701 agaaaataat ttaacaaaac cagcattagg atttataaaa agcaaaggat taagcgaatc
    20761 tgacatatat tttaaacaag ttggaaaaga agaatatata tttgcgacta taaagcaaga
    20821 tggaaaagaa acttcagaag tactaaaaga catattacca gagactataa aatctgtagt
    20881 attcccaaaa gctatgcgtt ggggaggaaa aaatcttaaa tttgcaagac ctataagatg
    20941 gatagttgca ttgttaaatg ataaagtatt agagtttgat ttagaaggaa taatatcttc
    21001 taatgtaaca aaaggtcata gatttttagg tgaaagtaca atagaagttg attctataga
    21061 agattatttt gaaaaattag aaaaaaatta tatagtttta gaccaaaaca agagaaaaga
    21121 aatgataaga aaacaatgta tagaagtggc aaattcttta ggtggagaag ttgaatttga
    21181 tgaagattta cttgaagagg taacttactt agttgaatat ccaactgctt tttatggaga
    21241 atttgatgtg gattatataa aactaccaaa agaagttgtt ataactccta tgaaacaaca
    21301 tcaaagatat tttccagtac ttaaagaagg aaaactgtta cctaacttta tagctgttag
    21361 aaatggtgat tcttacagaa tagacaatgt taaagctgga aatgaaaaag ttttagaagc
    21421 aagacttgca gatgctttat ttttctatag agaagatact aagagaaact tagaaagcta
    21481 catagaaaaa ttaaaaactg ttgtattcca agtaaaacta ggaactattt acgacaaaac
    21541 tttaagactt gaaaaattaa gtgctgatat acttgatagt ttaggtttag tagatgaaaa
    21601 ggcagatact ataagggctg ctaaattatc taaggcagat ttagttactg gaatggtagg
    21661 agaatttgat gaattacaag gttttatggg aaaagaatac gcaaaagttg gaggagaaaa
    21721 tgatgttgta agtgaagcta tttttgaaca ttatcttcca agatttgcag gagatatact
    21781 tcctaagact aatgcaggag ttgccttatc aatagctgat aagttggatt caatagcagg
    21841 tttctttgct ataggaatac aaccaacagg ttctcaagac ccatatgctt taagaagaca
    21901 agctttaggt atacttagta tacttatgga cagaaaagtt gctgtggata taaaagtact
    21961 tattgaacat gctttagaca attattctga attagatttt gataaagaag aagttgttga
    22021 gcaaataatg aatttcttca atgagagagt aaaaaatcta tttaaagatt taggaataag
    22081 atatgatgtt gttgatgctg ttttaagttc tgatataaat gatgtttctg atatgcatat
    22141 gagagcttta gagcttaata attggctaga aaaagatgaa ttagtggaga tgcttactgc
    22201 atttaataga gtttctactc tagctcaaaa agcagaaagt gataaaattg atgaatcttt
    22261 attaaaagaa gatgctgaaa ttaaacttta caatgaattt aaacaagtaa aggtaaaagt
    22321 taatgaatta ttaaaggata agaaatatgg tattgcttta gatgaatttg catctttaag
    22381 accagtagtt gataatttat ttgatacagt tatggttatg gataatgatg aagctattaa
    22441 gaacaataga cttgctttac ttaaacaagt atatgatact atgctagaaa tttgtgattt
    22501 atctaaaatt gtttataaat aaaatttaaa ctatttataa gaaataaggc tgtctcaaaa
    22561 tagagatagt cttatttttc attgttgaga tgatgaaaat atatttgatt tcatagaaaa
    22621 agagtgtctc ataaactaaa aagttcattt tgagacaccc atgttttttg taagtaaata
    22681 taataaatta tattttatac ttatcagtta ttttgtttaa aatattatca tttgaattta
    22741 cccattcata aaaattgcct gatgatgcgt ccgatatttt ataaagcatt tcaggaatat
    22801 cactagcttt aaaatctcca agtgattttc ccaatctagt cttacctatt tcaaaactgc
    22861 ctccaacaaa taattcaaat gcatctgtag atataccatc aacattcttc tttttaccac
    22921 ataaacctat gctaccaact tgatgaacac cacatgaatt taaacatcct gatatatata
    22981 acttaggtgc tttatttaaa atttcttcat tattttgtaa tctaaaataa tctatgattt
    23041 catgtaacat tttctgacta ttttgtatgc ccatttgaca aattggaaca cctatgcaag
    23101 atatactttg ttctaattga gaattacaac taatagtttt acttatttct aatattcttt
    23161 ttgcttcatt tccatttaaa tttaaaatat ataatccctc tgtcattcct agtctaatca
    23221 taggattttc aacattatct aattctttaa gtaaagtact taaatccttt gttaacaaaa
    23281 tacctccaac tggatgtata tatacagtgt ataaaccatc ctgtttttga ggtattagtc
    23341 tagaatcctg tatatctatt ttaactccag gtttagagta atctatttct tcaatattga
    23401 gttcaagact accattttct ttttctaatt tatagtattc tttaaatttt tctaaaaata
    23461 gttcttcacc aagtgattcg accatatatc taaccctagc tctgttttta ttttcataat
    23521 ttccataatc cataaagaat ttaatcatac cttcaacaca atataaggca tctttcggct
    23581 ttataagttc atcaagttct aaagcaactt tgggttgttt acctagacct ccacctaaga
    23641 aaactctaaa atatggttca tcatttttca aagtagcaac aaatcctaag tcttgtatac
    23701 tacaatgagc agtatctgtg taacaattag aataagaaac ttttaatttt ctaggtaaat
    23761 ggtatgtagt agcatttttg ataaaatatt tatcagtagc aactgcatat ggagtaacat
    23821 caaagacttc actagggtca actccagata atggagataa tccgacattt ctaggataat
    23881 taccaccacc acctctagtg aatatatcat tttttattcc ttcttccatg atatcaacaa
    23941 ttgaatttat agataaatcg tgtaattgta tagcttgtct agttgttaag tgtatcttat
    24001 ctaagttgtg tttaatagct atttcataga ttttattaag ttgatactga gataaaactc
    24061 cagcagatac tctaagtctt atcataaaag attttttgtc tctttgagca taaacaccat
    24121 atccaccaga aaatccctta tattgcattt tatttatttc tccatttaaa aattttgaag
    24181 aatattcttt aaaattaggt agttcattta acaatatctt tttaaaatta tccattattt
    24241 cacctcattg ttagtcttgt ataaaaat
//
LOCUS       DAEMHZ010000050        23838 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000050 DAEMHZ010000000
VERSION     DAEMHZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.050"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..141
                     /locus_tag="KPK72_001912"
     tRNA            68..141
                     /locus_tag="KPK72_001912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="KPK72_001913"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="KPK72_001913"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="KPK72_001914"
     CDS             540..2558
                     /locus_tag="KPK72_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2489131.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3063..5075
                     /locus_tag="KPK72_001915"
     CDS             3063..5075
                     /locus_tag="KPK72_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2489132.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGIINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5317..7230
                     /locus_tag="KPK72_001916"
     CDS             5317..7230
                     /locus_tag="KPK72_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489133.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKAVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYLSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            7223..9442
                     /locus_tag="KPK72_001917"
     CDS             7223..9442
                     /locus_tag="KPK72_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2489134.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDADNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9551..10381
                     /locus_tag="KPK72_001918"
     CDS             9551..10381
                     /locus_tag="KPK72_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG2489135.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10371..13838
                     /gene="addB"
                     /locus_tag="KPK72_001919"
     CDS             10371..13838
                     /gene="addB"
                     /locus_tag="KPK72_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG2489136.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13838..17665
                     /gene="addA"
                     /locus_tag="KPK72_001920"
     CDS             13838..17665
                     /gene="addA"
                     /locus_tag="KPK72_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG2489137.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIENFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17711..18922
                     /locus_tag="KPK72_001921"
     CDS             17711..18922
                     /locus_tag="KPK72_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG2489138.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18909..22436
                     /locus_tag="KPK72_001922"
     CDS             18909..22436
                     /locus_tag="KPK72_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2489139.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLLKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSRVKIEYS"
     gene            22567..23820
                     /locus_tag="KPK72_001923"
     CDS             22567..23820
                     /locus_tag="KPK72_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2489140.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT        10243 a         2324 c         4203 g         7068 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg taattattta taattttggg taaaaacact ttgatatatt
      481 ttcaaaagat aaaagattaa aaaacaaggt agaaatttat gttaagtaag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag gaattctttc aatggcaatc gtcacaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatagtt ttaggtagta cctcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctttaaacta caatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cgcaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgcgcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctagag
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagat aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaacacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggcc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgcgaact
     1801 taaaatctcc aatacttata acaccaaaga catatgtatc tgcataccat aaagaaaact
     1861 tagaagctaa atcggcaaat aaggtatata aaataggtgg aggattgact tctaaggtaa
     1921 tgagctctat agcatcatca ttatctaaac acaatacaac tccaacagaa ccaggaaata
     1981 gtgggggcaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt aaagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagataca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg aggaactact aagactgtag ctacaaatat attaaataga attttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatctg caattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aagtgcgaaa tgaggtatat
     2701 acagtctttt tatgattaat tgtaaaagga tttatattat atgaagaatt tacatatctt
     2761 aaggctacag ttaattatca agagttggag tattcatttg taatacacaa taaaagctta
     2821 attactatat tttattgtgt gtttgtgtgt tttttgtgga tttttatgtc aataaatgat
     2881 atagtataga taattaaatg tattaaatat atttaatttt tttatttttt gagttttata
     2941 agtttatttt tatatattac ttatatatta tttatatttt gggttagata cactttgaca
     3001 tattttcaaa agttaaaaga ttaaaaataa agtagaaatt tatgttaagt aaggagatta
     3061 atatgagaag aaatacaaaa ttattaacaa cagggattct ttcaatggca atcgtcgcac
     3121 ctacaatggc atttgctact gaatctaatg ctatggaaaa taacgctgat ttaaatataa
     3181 acttagagaa aaaaagtatc gttttaggta gcaaatcaaa agttagtgtc aaatttaaag
     3241 aaaaaccaga tgcagatagc attacattaa agtataaatg ctatgacatg ccattgaata
     3301 caactctaaa ttacaatcaa tcaactgggg catatgaagg aattatcaat tataaccaag
     3361 acccagaata tctaaatgtt tgggaactac aagggataac aataaacagc aaaaataatc
     3421 ctaaaacttt aaacagacaa gacctagaaa agatgggatt aaatttaaaa gactataatg
     3481 taacacagga atgtataatt gaagacataa cttctagaaa agatgtaaat aaatatttga
     3541 gaaaaacttc ttcacctatt acagaactta caggaagtga tagatatgaa acagcagtta
     3601 aaataagtaa agagggctgg aaaaatggtt cagataaagt agttataata aatggagatg
     3661 taagtataga tggcattata tcaactccac tggcaaccac atataatgca ccaatacttt
     3721 tggttgaaaa aaacaatgtg cctaatagtg taaaatcaga attaaagcgc ctaaacccta
     3781 aagatataat tataattgga gatgagaatg ctatttctaa aactactgct aatcaaatta
     3841 aatcaactgt aaatgctagt caaacacgtt taaatggttc taatagatat gagacatctt
     3901 tattgatagc aaaggaaata gataaaaatc atgatgtgga aaaagtatac ataacaaatg
     3961 ctaatggcgg agaagtggat gcacttacta tagcagcaaa agcaggtcaa gacaagcaac
     4021 caattatatt aactgataaa gatagtatta cagacaatac atataaatgg ttaaagagtg
     4081 aggatttaca aaatgcttat tttataggtg gtcctcaaat gatatcaaca aatgttataa
     4141 ataaggtaaa tggaataact aaagatagtg ttactaataa tagagtatac ggagcagata
     4201 gacacgaaac aaatgcaaac gtaataaaaa aattctatac agatgatgag ttagaggctg
     4261 ttttagtagc taaatcagat gtacttgttg atgctttagc agcaggtcca ttggctgcga
     4321 acttaaaatc tccaatactt ataacaccaa agacgtatgt atctgcatac cataaagata
     4381 atttagaagc taaatcagct aataaggtat acaaaatagg aggaggattg acttctaagg
     4441 taatgagctc tatagcatca tcattatcta aacacaatac gactccaaca gaaccaggaa
     4501 atagtggggg caagacagtt atgattgacc cagggcatgg tggttcagca cctggaaatt
     4561 catctggagg aatgattgaa aaagattaca atttaaatac ttcacttgca acaactgaat
     4621 atttacgttc aaagggattc aatgtaataa tgacaagaga cacagataag actttatctc
     4681 ttggaaatag aactgctcta tctaattcat tgaaaccaga tttatttaca agtatacatt
     4741 ataatggctc aactaataaa caaggtcatg gtgtagaagt attttataag cttaaagata
     4801 aaaatggagg gactactaaa actgtagcta ccaatatatt aaatagaatt ttagagaaat
     4861 ttaaacttac aaatagaggt ataaaaacaa gagtacttcc tagtgattct acaaaagatt
     4921 atttatacgt tttaagaagt aatgatatgc cagctgtact tgtagaatgt gcatttttgg
     4981 ataatgaaaa tgatatgagt ttaataaact catctgcaaa agtaaaagaa atgggtacac
     5041 aaataggtaa aggaatagaa gattcattaa aataaatgga aataaaaata tttaaaaact
     5101 taatgtaaat ttatagttgt aaatttatac taaagtcaaa aaatgaaata taagtgtatc
     5161 aatagtaaga ctgtatgtac atttgcacat gtgaatgcaa gtatatatac agtctttttt
     5221 tgtagttaaa tgcaataatt tgattgtgtt ttgtaaaata cacaatatga tataattaga
     5281 taatatacac ttaatttaat tgaggaaggt gattatatgg aatatttatt tgaaaaaagt
     5341 agtttaaaag aatatggaaa tgaagcttta gataaatata gagaattatt agataaaact
     5401 gataataatt caaggtgtat tcttttgtta gtaccaaaca ataacacaaa gatacgatat
     5461 gaaagggctc taagattaaa ttatagtgaa gaactgaaaa taacaacata tataagtttt
     5521 gtgaagaaag aattagttaa atactggcca ctaataatag aaaaatgtga tgaaataagt
     5581 acaaaggctg tatctccaac atttatatca aatagtttaa gtgaatattt aatagtgcaa
     5641 aaagttaagg aaaaaaggat acaagaagga tattttgaag atattacagg aaccaataaa
     5701 tccatagcaa atagtataat gactaacata aataagtctg catttaattt aattgatttt
     5761 gattttattg gagaaaaaat atatttatca aagaaaaata aagatagtat atatagattt
     5821 tcatacactc aaatggatga aattatatca tattatataa attccttact atcaaattca
     5881 attttagata atgcattaag catatattta tacaatcaat acctacttaa tgatgatttt
     5941 tatttaaaaa acttatttac agaagtgaga tatataattg tggatagcct tgaaagctca
     6001 agtaatgcag aagttgattt tataacagaa gcattggata atacattaca aagttatata
     6061 tattttgatt atagtaggga ttattctgta tttaataatg tggatataga ctatataaat
     6121 gagaaaataa tatcaaaaat taagcctaaa gaagaaaata ttaaaaagaa ggagattaga
     6181 atagaagatt tatacttatt acctgcaaat atagaattaa atcaatcaag tcaattatat
     6241 aatgaaatgt tagatttaat aagtgaaaaa gttatatctc ttatagaaga agatgtatct
     6301 ccaagagata tagcaattat atctcctata aacaatagta tattggagca tcaaattaga
     6361 gattctttga tagaaaaaaa tatagatgtt tttagtacta agaaggataa aaaagctgtg
     6421 gattatccat atgggaatgc attggtagtg gctacatgta tattttatgg atatttagat
     6481 tttataaaag atgatgaata cttaagcttt ttagaaactc ttttagaaat aaataggata
     6541 aaagcattta agatttttaa tgaaacaaga catttaagta ttgataatat agatgaacat
     6601 agtcagtatc gtgatatttt acagtatatt gaagaaaaaa agaagagtga tataaaaata
     6661 catgaatttt taactcaatt ttacatagat aaaatgttaa atttaaaaga aggtaaaaag
     6721 aatgttgggt tatgtaaaaa gataatagct gaaagtgagt cttttagtga aagtttaagt
     6781 ctacttggga tgaaaaaaga aaagatattt gtagaagctt taaaaagtac aataaacgat
     6841 tattactctg ttgtcgatat agaagagttg aaatccaaag acaaaatagt aataactact
     6901 ccttattcat atatatcatc aaatatagat agaagtatac aaatatgggt agatatagga
     6961 agtaatgctt ggaatatgaa aatagaaaaa gatataagta atttaattgt acttagaaaa
     7021 tcttttgaag aaaaaaagat ttatacaaat gaaatggaag aatattataa aaaatactat
     7081 ctatacaata cagtatataa tttgttatta aatgcaaaga aaatctatgc atttaaaagt
     7141 gaatatgcga ttaatggtta tatccaagag agtatattat atagtatact tttaaaaatc
     7201 tcacataaag gagataaaaa ttatgattaa tataaattat agagaagacc agataccaat
     7261 aataaattac gataatggta ctatggcagt accagcagta ccaggtgctg gaaaaacatt
     7321 tataataaca aatttagtag ctaaactatt acttgaacag aaacataaga gtggtaaaat
     7381 attaattctt acatatatga atagtgctgt aaataatttt aaaggtagaa taagaaagat
     7441 tcttgaagag aataaaatag atgatactaa ttcgtatgaa gtaatgacta tacacagttt
     7501 ggctgtaaaa ataataaagg aaaagccaga agttgtaatg ttaagtgagg attttaacat
     7561 agctgacgat ttgcaaaaaa gtattatatt aaatgaatgt ataagtagat ttagatttga
     7621 aggtggagaa agagctttta gatggttttt aaaggagcaa aaagatgaac gctggaaaga
     7681 aataactctt gaagcctggg aaagaggttt ttttgaattt gcaggaaatg caataagtga
     7741 acttaagtat aagggaattt ctccagataa ccttgaagaa atattgtcaa gaggacacaa
     7801 aggaatgctt aaaattattt taccaatata taaattgtat gatagaaaat taaagcaaaa
     7861 tggattgcta gattatgatg atatattaat tttagcagaa aaaactttaa gcttagacga
     7921 agggcttaga aagaagtttc aaactagata caaatatata tttgaagatg agtgtcagga
     7981 ctcaaatgaa attcaaggta atataataaa aattatatct agcgaaaata aaaacctagt
     8041 tagagttgga gatataaatc agagtataac aggaacattt tcatcatcag accctaaatt
     8101 ttttaaagaa tttataaata gtgcagattt ctgttatagg atggatatgt caaatcgaag
     8161 ttcaaaagat atacttgatt tagcaaacac tcttgtcaag tatgttacaa aggaatttag
     8221 acagaaagaa tgtagacatg ctcttgaaga tatgcaaata aaaactgtgc caaatggaat
     8281 gggatataaa gaaaacccta atccagaaca ttataatata aatgtaaagt ggtatgagag
     8341 ttggaaaatt gaaatagagc aaactgctag atacgttaag ggtataaaaa ataaatatcc
     8401 agataaaagt ataggtatat tggttccatt taatgaacaa gtgacacaag ttgctaaaga
     8461 acttagagag aataatctaa cctttgaaga attaggacca aattctttga ataaaaggaa
     8521 ggttataaac aacatagcat atattattga ttttatgttg cattgtgatg atgtagaaaa
     8581 gttaatagtt gtacttaata aagtttttat agatgctgat aatgaagaag aaataaagta
     8641 ttttttagaa gaattaaaaa actattctac cgaagaaatt atttataatt ttgaatttag
     8701 taagttagat aatgatttaa tagaatttaa agaagaaagt gatatatact taagttttaa
     8761 aagaggaata gatattataa aacagatatt agaatatcca ataataaggt tagacctctt
     8821 agttttgttt attgggaaaa aacttaattt agaaaaagaa gataaagctg tactggatta
     8881 tatatctttt tatataaagt atctagtatc agaaaatata aatatggatt tagaagatgt
     8941 atataattta ttgtttgatg taaagaacag ggtgtttaat catataatag atgtggtata
     9001 tgagataaat ggatatgaac cagagcccgg tagtataaca ctatgtaact atcataagtc
     9061 aaaggggatg gaatgggatt gtgtgttttt actaggtctt gttgaatata atttccctga
     9121 caatattaat caaaagtttc agagtgataa atggtatcta aaagaaaaat ataaaaaccc
     9181 tatggcaatt attaaatcag aagttgaagc tatattaaaa ggaagtattt caacagatta
     9241 tgctcataaa acgaagattg attctataaa tgaaaagatt agacttttat atgtagggat
     9301 tactagagca aaggaaatgt tagtactttc aggaagtgct tatagagatg aaagtgatat
     9361 agggaataag agaaaagagc aaaaaccatg tatatattta agcagactta atcaacatat
     9421 tattgaaaaa aggtcaaatt aatatataat aagttattaa aagattgctt gaaatatatc
     9481 taaaagggga taaaacaata tgtacagtaa gtaactacta aaaaataaag ttaagagaga
     9541 agtgatatga gtgaacgata aattaaaata tttttcttat agtcaaaatt caattaacac
     9601 atataaatca tgtccattga aatttaaata taaatatatt gatagaataa attggaaaaa
     9661 tgatgatgtt ggtagtagag agtattatga aactttaaaa acaggaagag attttcattt
     9721 gatttgtgaa agatatttta gcaatatacc tcttggaatc tattttaatg aggatgataa
     9781 aaatagtaaa aagtttttaa agtggatgga aaatataaaa aaagttgttc ctattgaaaa
     9841 aggaaaaacg tatttgccag aatatgaagt aaggatgaca ttaaatgggg atataataca
     9901 ggcaaagtat gatttagttg taattggaaa taacaatata gaaatatggg attggaaaac
     9961 cgagtctaaa aagattgact ataaacacgt agaaaataga atacaaacta ttgtttatat
    10021 gtttttagca aaagaagtaa tacctaaagt tttaaaaatg gacataaatg ttaaagatat
    10081 aaatatgaga tattatcaac ctgaatttga tgacttacca attaccattt tatatgatga
    10141 agaaaaacat gaagcaaata gaaataaaat tcaaaattat ataagtatga taaaaaacac
    10201 taattatgaa gaacatacat acgaagacaa tctatacaat gatatagaaa aggtatataa
    10261 taaagaagaa cgtatgtgct atagaaataa aaagcattgc aagtattgtg aatttaataa
    10321 gttatgtaat ggcaaagaaa tagattatag tattttggag gcggaaattt atgggactta
    10381 ggtttgtatt aggtagaagt ggaagtggaa aatccaccta tatactagat gagattaaaa
    10441 aagaagctca aaaaaatgaa acgacatcaa ttatactttt agttccagaa cagtacacat
    10501 ttgaggctga aaatagagta agtaagctat tcttaggaaa agaaaaagat aagtatctaa
    10561 gagttagggt acttagcttt aagacactaa gtaatatagt tttttctcaa gtaggaggat
    10621 tgacagatgt aaatataaat tctagtggaa aagcaatgat ggtatataga gctattgaag
    10681 atgtaagtga agaattaaat gtgttttcaa aatcaaaatc gcagtctggt tttgtaagtt
    10741 caattacaga tatgatatca gaaatgaaac aatacaatat aagccctgaa atgttagaaa
    10801 atatatctgg agaattggat aatgaaactt taagtttaaa attaaaagat ataagtaaaa
    10861 tatataattc ttttgaaggg aaattgcatg aaaattatgt agatgctcaa gatatgttaa
    10921 cttcattggc aagtaaaatt gaattaagca gttatttaga tggagcttgt gtttatatag
    10981 atgaatttac aggttttaca ccaaatcagt acaatgtcat aaagagtata ttaaataagt
    11041 ctaaaagtgt taatatctct ttgactgtag atgatatcaa ctatattggg tacagtaaat
    11101 ctgatatgtt ttctagaacg aaatttactt attcaaaatt gactcaattg tgcaacgaag
    11161 aaggcataaa aatattgcca caagtaaatt taaacacagg tgttataaaa agatttgaaa
    11221 aagttaagga attacagcac ttagaaagat tttataatgc ttatccatat aaaatatatt
    11281 caaatccaac tgaaaatata aagataaaag agtttaacaa tttatattca gaggtggaag
    11341 agatagccag agaaattgta catttagtta gagataagaa tgtcagatat agagatataa
    11401 ccattgctac aagagattta aatagatatg attttttagt acactctata tttaatgaat
    11461 ataatatacc aaactttata gataagaaga gagaggcaaa aagtaatcca atagttattt
    11521 taattatttc agctttagaa atgaagaata gacgatatgg gtatgaaact atgtttaggt
    11581 atttaaaaag tggacttata ggaattgata atgatgatat aaatttacta gaaaattatg
    11641 tgttggctaa tgggattaaa ggtaaaaaat ggtttgatga aaaatgggat tatagaataa
    11701 ctcaaagtct ttcaggtcaa gaaagtgaat ttgaactgga attaaaagag aaaataaatg
    11761 aaataaaaaa tagagtatta gagcctatag taattttaca agaaaaatta agaggaaaaa
    11821 atagagtaaa agaaatctgt agatatatat atgaattttt attagatata aatatgccag
    11881 aaactataga aagtttaata gtaaatttta aggataaagg ggaacttgat gttgcaaatc
    11941 aatactcaca agtttgggac atagtagtgg acatattaga ccaaatggtg gaacttatgg
    12001 gtgatgaaat aatttcttta gaaaaattta taaaattaat aaccttaggt tttgatgaat
    12061 atgaattagg actggttcct cctagtatag accaggtact tgtaagtagt gtggatagaa
    12121 tgaaaaatcc agacacgaaa tatttatacc tagttggaac tactgatggt gtgtttccgc
    12181 tgattacaaa ggatagtgga atattgagtg ataatgatag ggaaagtctt ggaaacaaag
    12241 gaatcgaagt tgatattgat agtaagacta ggagttttga agaacagttt cttgtatata
    12301 aagctcttac ttcgacatct aagaatttaa ccataacata tccaatatca gaccatgaag
    12361 gtaaaacgct tagaccatca ataattatat caagacttaa gaagatattt ccaaatatag
    12421 aaaataaaag ttatttagtg gaagaaaata aaagcactga taaagatata cttaaaaaaa
    12481 taacggttaa atcccctaca tttaatgaac ttataaacgt aataaaaaat tatgacagtg
    12541 atggttacaa tacagaagag attaatagta tttggttaga tatatatagg tactatttaa
    12601 aagatgaaat ctatagttct ataacaaaaa aagttataaa aggtcttagt tatactaatc
    12661 aagttcataa gattgaagag aaaaaaataa ggtcattgta taaaagtaat tctcttagca
    12721 tatctaggct tgaaaaatat gctgaatgtc catttgctta ttttatacaa tatggtctaa
    12781 aagcaaaaaa gcgtaaagag tattcattta caccaccaga tttaggaact tttattcata
    12841 atattttaga taggttttca aaagaacttt tacaagataa tttaacttgg agagatattg
    12901 atgaaaaata tatagaatta aagataggta taatagttga tgaaataatt ttaaagatac
    12961 caggatatat attaaatagt tctgagaggt ataaatacct tgcatataga ttaaagaata
    13021 tgttgacaac tgctataact ataattagtc agcaaataaa acaaggttca tttgaaccaa
    13081 tagattatga agttaaattt ggcgacaatg gaaaatatcc acctataaaa atggtgttag
    13141 aaaatggaca agaagtaagt ttaattggac aaatagatag agttgatgaa tttgaagaag
    13201 gagaaaataa atatataaga ataatagatt ataagtcagg caataagtcc ataagtctaa
    13261 cagaaatata ttatggtcta caattacaat tacttgtgta tttagatgca atccttgaaa
    13321 gtgcaaagga tgaagatata aatataaatc cagctgcaat actatattgt agaataaata
    13381 atccaatagc aaaatttaat gaagataaag atgatgaaga aatacaagaa gcaatattaa
    13441 aagaacttag aatgaagggg cttgtagtaa aagactctca catagttaaa gagatggata
    13501 aatcattaat agatggagaa aggaaaaact ctttagtaat accagtaggt cttactaagg
    13561 atggaaatgt aggtaaaagt acttcagcaa taagctatga ggatttcaaa ctgcttagaa
    13621 aatatgtaag gcatgctata aaggatttat gtgaagaaat gttaagtgga gaaatacgta
    13681 tagctccata taaacataaa gatggaacat catgtgactt ttgtgattac tctgctatat
    13741 gtcagtttga ttcaactata aaagataaca agtataaaaa tttaaataat aagagtaatg
    13801 aagagataat aaaaatgatg aaaggagatg ttaattagtg agttctccta aatggacaaa
    13861 agagcaatta gaagttatag aaagtagaga atgcaattta ttggttgctg ctgctgctgg
    13921 ttcaggaaaa acagccgttc tagttgagcg tataattcaa atgataacaa gtagagaaaa
    13981 tccaattgat atagataagt tattagttgt tacatttact aatgctgctg cgtcagaaat
    14041 gagagagagg ataggggatg ctataggaaa agccttagat gaaaacccag aaaataaaca
    14101 tttacaaaac caacttgttt tattaaataa atctagtatt acaacaatac actcattctg
    14161 tttggatgtt ataaaatcaa actttcatag aataaactta gaccctaatt tcagaatagg
    14221 agaccaaact gaatgtgcta ttttgaaaca agaagcaata gaggaagtat ttgaagattt
    14281 atatgaagaa agagatgagg ggtttttaaa tttagttgaa agttatgcag aaagaggagg
    14341 agataaagaa gtacaagata ttatcttggg aatatattca tttgcaatgg cttctcctga
    14401 acctaaaaaa tggttgattg actcagcaga acgatttaat attgatgaaa attttgattt
    14461 ttcacagtct atttgggcta gagctatatt agacactgta aaaatagaga ttaatggtct
    14521 ttgtctaaat atggaaaggg cattaaaaga agtggaatca atagaagaat tagaaacttt
    14581 tgcagaaaag ttatctgttg aatataaaaa aattgctgac atttcacaag catgtaataa
    14641 atcttgggat gaggcatata aaaagatggc aagtatgtcc tttgaaaatt atgtaaaagg
    14701 agtaaaaaga atttcaaaag atgctccttc gtatataaaa gaatcaaaag aaaaggcaaa
    14761 aactataaga gataagacta agaagtcttt agaaagtata gtaagtgcta cattcaataa
    14821 agataatgat tctattagag aagaaataaa atatctttat aacatagtta aaccaatatc
    14881 tagtgttgta ctgagatttg aagaggaata tagcaataag aaaagagaaa aaggaataat
    14941 agattttaac gatatagaga attttgcatt aaatatactt acagatgtag atgaaaaagg
    15001 taatattgta ccttctgata tagcagttgg atatagaaac aagttttatg aaatatttat
    15061 agatgaatat caggatagta atctagtgca ggaagtactg cttaaagctg ttgcaaatac
    15121 agaaactcca aatagattta tggttggtga tgtaaaacag agtatatata gatttagaca
    15181 agcaaagcca gaattatttt tgcaaaaata taataactat aatgataaaa agggaagctc
    15241 ccatagaaaa ataatgcttt acaaaaactt tagaagtaga gaagaagttg tagatgctgt
    15301 aaattatata tttgaaaata taatgaatga gaatatagga gaaattgaat atacagaaaa
    15361 agaaagacta aatttaggtg caaattttaa tgtggataca gacgagaaat ctattattgg
    15421 tggagcaacg gagatacacc taatacaaaa agataataaa cttgatgatg atattataaa
    15481 tgataaagac gatagaatta ataacaaaga agatgaaata gaggaagaag aaaaattaga
    15541 taatatacaa cttgaggcta gaatggttgg aaacattatc aaagatttaa tgaaagtcaa
    15601 tgaagatgga aaaattcaaa aggtctatga taagggaata gatggatata gacctgtgga
    15661 atttagagat atagttattc ttttaagagc tacatctgca tgggctccag tgtttgcaga
    15721 tgaattgatg aatatggata taccaacata tgctgatgtt ggagtggggt attttgatac
    15781 gattgaaata aaaacaatat tgtcattact tcaaataata gataatccaa tgcaagatat
    15841 acctcttata tctgtattaa aatcacctat atttggattt acaccagagg atttgattga
    15901 tattagagtt cagagtaaag ataaaatttt ttatgaggtt ttaaaaagta cagcagagta
    15961 tgatggattt acagattctc aaaatgaaaa tgagtctgag tttataccaa gtgaagagtg
    16021 cataaacaaa agtaaagatt ttttaattaa gctaaaggaa tttaaagaaa agtcaatgta
    16081 catgagtaca gatgaattta tatggtactt gtacacaagg actggatact atgcctatgt
    16141 tggagcattg ccaggtggaa gtcaaagaca agctaattta aaagtattgt ttgagagagc
    16201 aaaacaattt gaagaaacca gtcttaaagg aatattcaac tttgtaaatt ttatagagaa
    16261 attaaaaaaa tctagttctg atatgggaag tgcaaaaaca cttggtgaga atgcaaatgt
    16321 tgttagaata atgagtattc ataaaagtaa aggtctagaa tttccagtag tcatatgctc
    16381 tgcaatgggt aaaaatttca atactcaaga ttttaaaaag agtattttat atcatcataa
    16441 tttagggtat ggacctcaat ttgtagacta tgagagaagg atttcttttc caagtatagc
    16501 caaagaagca ttaaaaagca aaataaatat agaaaattta tcagaagaga tgagagtttt
    16561 atatgttgca tttacaagag ctaaagaaaa attgattata actggttcta ctagaaatat
    16621 acaagatagt ataaagagat ggtcaaatgg aattgaaagt ttagatacaa tatcacaata
    16681 cgagatatta aaaggtaaaa attttttaga ttggataatg ccttgtgtat taagacatag
    16741 ggatttatct aatctactag aagaagtagg attagacgca gtttttaatg ttgaacataa
    16801 ttcaaaatgg tatggaaagt tgtggaataa gaatgatatt ttagtggaaa agaagagtga
    16861 tgaagaaaaa gaaagtattg aagaaattct tgaaaaaata gatgtagata atcctgacag
    16921 tgattattac agtgaaatag aagaaaaatt aaactatata tatccatatg aatttagtac
    16981 aagaaaacct gctactatat ctgttacaga aataaaaaag atacaaaata attatgaaga
    17041 agagttaata aatactatat ttgaacaaaa agtaatatta aaaaagcctc tatttattca
    17101 aaatgaagaa gaaagagaaa aaataagtgg aacagaaaga ggtacaatag tccatttagt
    17161 tatggaggtt ttagacttaa aaaatgtaag tagtgtaaat gatataaaat cacaaataag
    17221 aggttttgtt tcaaaaggta taataacaga aaaacaagct agtatagtaa atccatataa
    17281 aatatataaa ttttttgcct ctaacatagg aaaaagaatg ttgaatgctg aaattataaa
    17341 tagagaaaaa tcaatttatg cacaagtaaa tatgaaagac atatatattt atgaaaagct
    17401 tataaacaat gatgataaaa aactttatga taatgaaagt gtcatgttaa gaggtatagt
    17461 tgatgcttat tttgaagaag ataatcaaat agttttggtg gattataaaa cagattttgt
    17521 aaatgaggaa aatataaatc aaataataga gaaatataaa aaacagttgg atttatatgc
    17581 agatattatt gaaactttaa ctggtaagtc agtaaaagag aaatgtatat atttatttgg
    17641 agttgatgaa gctgtttgtt attagttttt atatataaat taaacctaaa agatagagga
    17701 aggggaaaaa atgagattta ttcatacatc agattggcat ctagggaagt ccctagaagg
    17761 ccattctaga attgaggaac aagcaaagtt ttgtgaggaa tttataaaaa tagtagaatc
    17821 aaatgaaata gatatggtta taattgctgg agatgtatat gatacttcta atcccccagc
    17881 acaagcagaa aaactatttt atcagactgt atcaagactt gccaataatg gtcagaggtg
    17941 tgtattaata atttcaggaa atcatgataa tccagagaga ctctctgcaa taacacctct
    18001 agcacatgaa cagggaatac ttatatatgg ttatccatta agtgctacga tagaagctaa
    18061 gtataaaggg tttgaaataa catatgcaac tcagggatgt acaaaactga atataaatgg
    18121 agaaaatatt gtggttgcta cattacctta tccaagtgaa aaaagattaa atgaagtttt
    18181 ttcaagtgaa gatgagtttg agaaacaaaa aaattattct gaaaaggtag gagatatatt
    18241 tagaagttta gaagaaaatt ttagaagtga tactataaat atagccgtat cacatatatt
    18301 tgtaataggt ggagagagta ctgaatcaga aagacctata caattaggtg gaagtttttt
    18361 agttgaaaga aaagatttgc cagaaaaggc tcaatatact gcactaggtc atttacataa
    18421 acaacaaaaa gcttccgaac gtttaaatgc atactattct ggttctccac ttcaatatag
    18481 caaggatgaa agagcgtata ccaaaggggc atacatagtg gatataaaag ctggtgaaaa
    18541 accaataatt gaagatgtat attttaataa ttacaaacct atagaagtat ttaaatgtaa
    18601 tggaatagaa gaagcactag atatatgtga gcaaaatcaa gatagagaga tttggtcata
    18661 ttttgaaata aatactgatg agataatatc acagaatgaa attaaaaaaa tgaaggaact
    18721 attaaaagac ataatagaaa taaaaccaat aataacatct tgttatgaac aagaaagtgt
    18781 agatataaaa gaaaagtcta tggcagagtt gtttagagag ttctattctt ttagtaaagg
    18841 tgtagagcca aaaggagaac ttatggattt atttctagat ataataagtg aagaaggtga
    18901 atcagcagat gagaccaatt agattagaac ttacagggct taatagctat attgataaac
    18961 aagttataga ctttgaaaaa ttaattgaaa gaggtttgtt tgggatattt ggaactactg
    19021 gaagtggaaa atctacaatc ttagatgcaa taactattgc tatgtatgga aatatatcca
    19081 gaaatacaaa agaatatata aacagtgtgt gcgataaggc aattatatca tacgagtttg
    19141 agataggaag caaaaacact agaagaagat acattgtaga tagaaccatt gctagaagca
    19201 aaacaggaac aaagacctct aatgctagat taatagaagt attgaatgat aatacccaaa
    19261 atgttttagc tgataaagtt gtggaagtaa atgaaaaagt agcacaggtt gtaggattaa
    19321 cggcaaatga ttttactagg tcagtagttt taccacaaga caaatttaat gagtttctaa
    19381 gattatcacg tgcagataga agagacatgt tagagagaat ttttaatctt gagaaatatg
    19441 gaagaagtct tggagaaaaa gtgaaaaaga gaaaaaatat acaattacaa aatttaaaag
    19501 atttgaaatc aaaattaagt caatatgatg gaattacaga ggaagtatat aacaatataa
    19561 accaggaatt aatagagtta aaaaatttag aaaaagataa gaataatgct ttagacctag
    19621 ctcaaaaatc ttatgaagaa agtaaaacag tatatgaaga acaattaaag ttagaaaaaa
    19681 atgaacttag aaaaaatgag cttgatttaa aaaatagtga gataaaagaa aagattgctc
    19741 ttgtagaaaa tgatagtaac gctaaaaagg tagacccata cataagttca gttcaaaatc
    19801 tagaaaaaaa gatagatgaa gatagctttg cagtaagtaa tttagaaaag aaattagcaa
    19861 tattaaatca ggagcttgaa gttacaaaaa atagatatga aaaaataaac aaaattaaga
    19921 atgaagaagt ccctaaattg agtgaagaaa aaataagatt gcaacaagca ataaaattag
    19981 aagaagagtt agttttatta gatagagaat taaaagagct aaaagaaagt ggaattaatt
    20041 taaataagac taaagttgaa ttagaaaaag taaagcaggt gtcagagtcc agaaaagatg
    20101 cagttactaa atctataaaa gaagttgaag gaaagattga taaagtaaat ataagtgcag
    20161 aattaaaaca aaaaatattt ctagcatatg aatacgagaa agattacaac aacgtattgg
    20221 aagaaaaaaa tcaaaagcta aataaactag aagaaatact caaagagaca gaaaatataa
    20281 atttaaaagt tagatatatt gataaagata agaatgatgt aaatcgtaat ttagaaaatt
    20341 tatcacttca cttggatgtt cttcttaaaa aatgtcctgg taagagtgct gatttacttt
    20401 taaaatctga atatgttact gaattaagaa ataaagcaaa taatactaaa gaaaatgaaa
    20461 ttaagaaaag cagtatacaa gatgaattaa agataatact tgaaagtaag tttaatactg
    20521 aaagggaagt aaatttacta aatgaaaaac ttgaaaataa tagaaagaac agagatgact
    20581 tagaaaaaga attagaagaa ctaaagtatt taaatttagc ctctgaactt agacgtgaat
    20641 taaaagaaaa tatgccttgt ccagtttgtg ggtcaaaaca tcatgaaaat catgatataa
    20701 ctaagtatga tgaaaatata tcttttgtca aagaaaaact ggagaaatta gaaaaagaaa
    20761 aaataagcat aagaaacaat atagaggaat taaatgctaa agtaagtggg tatttatcta
    20821 tagaaaaaat gaagactaaa gaacttgaag atgtaaaagg aaaactaggg gggattccat
    20881 ctagtcaatt attaaaaaaa cttgatgaag aacaaagaaa acttgcattg ttaaaatcta
    20941 atattcagga gtgggagaaa gaaaaagaga gtactgaaaa taaagtaaca ttagctaaag
    21001 aagaaaaaaa taaaattgaa aaagaagaat taaaaataag agagagtctg aataattata
    21061 agaaattaac aagagattta aacatagaaa tagaaggttt agaaaataaa tgtaaaaaac
    21121 ttaaacagga atacctagga ttaaagacga ttactaaagt ctctaatcta ttgttaaaag
    21181 taaaagaaat aagagagaat gaaaaagaat tagaattact aagtactggg cattcaaatt
    21241 tattaaaaaa tagagactca ctagatatta aaataagaga aaatgagagt aaactacatg
    21301 aaatagagat agaattaata aaggctagag aattatatgt agaaaagaaa ttgagtagag
    21361 ataataagta taaagaagtt attagcataa cgaaaggtga tttagctaaa aatcttttac
    21421 ataatgttga agaaaatata tgtaagatat tagaacaaga agaatcaagt aagaaaaaat
    21481 tggaagagca aagattagaa tatgaaaaaa acattgctga aaagcacaat atagatggta
    21541 gacttaaaac tgctaaggaa caatataaag accaaaaatc aacactaaat aaactattgg
    21601 cagaaaataa atttgaaagt atatatgcag taaaaagagc tttattagat actgatgcag
    21661 taaaaaaatt gattgaagaa atactagagc atgaagaaga acaaaagtta ttatcattta
    21721 aaataaaatc atcaaaagaa aaattaaatg gaagaagcat aaagaaggag tactttgaac
    21781 agttaaaaga tgaaatttat aatttgaaag tagaaatagg aaatatatcc aaagatattg
    21841 gagctaatca aaatcagtta ataacattaa aagactcttt agataagata aatgatttta
    21901 ataaacaact taaggtagtt gaacacaatg tggacttatt ggaagaatta gataaatgtg
    21961 tacaaggaaa taaatttgta gaatatgttt ctacaaacca attaaaatat atagcgttgg
    22021 aagcatcaaa acgattggat ggaataacaa aaggaagata tgcacttgaa attgactcaa
    22081 cactaaactt tgtaatgaga gataacttta atggaggaga aagaagaagc attgatactc
    22141 tatctggtgg agagacattc ttgacatctc tatcattagc acttgctctt tcatctcaaa
    22201 tacaattaaa gggaagtgca ccattagaat ttttcttttt agatgaaggt tttggttcat
    22261 tagatagtga acttttagaa attgttatag aatcattgga aagattgcat agtaataatc
    22321 taagtgtggg tataataagt catgtcgagg aacttaaaaa tagagttcct gttaagctat
    22381 tagtttcatc tagtgaggct ggaataggtt caagagtaaa aattgaatat agttagaaaa
    22441 tatatgttaa ttgaatatat tatctaatat ttccaaataa tatgtaattg tgatataata
    22501 aaaactaata ttaaagttat ttataattaa tattaatata aaaacatgta agtaggagtg
    22561 tggtaagtgt tggaatcgcc caataatttg attcagatta tttttttaat agtattactt
    22621 atagggtctg catttttctc agcatctgaa acggccttaa tgtcgttgag taaaatcaga
    22681 atcagatata tgcaagatga aggagttaaa ggagctaagt tagtaagttc attaatagaa
    22741 aatccaaata agcttttaag ttctatattg gttggaaata acgtcgtaaa tatagcagca
    22801 acatccatat caacatcttt gtttatagga ttaatgggag aaaaaggagt ggcacttgct
    22861 acagctgtta tgacagtatt agtattaata tttggtgaga ttactccaaa aacaatagca
    22921 gcaaataatt cagaaaaggt ttctctatta gtatctaaac ctataaaagc aattatattt
    22981 atattaagac caatagtatg gatatttaat ataattacaa atattatatt taaattattt
    23041 ggtataacaa ataaaggagc taaatcattc ataactgaag aagagctaaa aactatggta
    23101 aatgtaagtc atgaagaagg cgtccttgaa atggaagaga gagaaatcat aaataatgtt
    23161 tttgaatttg gagatatgca agctaaaaat gcaatggtac aaagaataga tatggtagct
    23221 atagatatgg aagatagtta tgatgagata atacaggtat ttaaaactga aaaattaagt
    23281 agaatgcctg tgtatgaaga aactatagat gatattgtag gaatacttaa tataaaagat
    23341 attatatttt tatctgatga agaaatagag tcatttgata ttaaaaatta tatgagagaa
    23401 ccttttttca cgtatgagtt caaaaagata acacaacttc ttgaagagat gaaacttgaa
    23461 aaaagtcaga tggctatagt tgtagatgag tatggtggaa catctggact tttaactata
    23521 gaagatttag tagaagtcat tgttggagat atagaggatg aatatgatga agaagaggat
    23581 gaaatacaag ttataaaaga agatgagtat attgtagatg gaagtactaa aattggtgat
    23641 gttaatgaac ttattggtgt aaacttggaa tctgaggagt ttgattccat aggaggtttt
    23701 ataattggac acctaagcag attgccagaa gaaaatgaag taattgaagt tgataatata
    23761 agattttgta tagaaagtat agaaaaaaat agaattaaaa aaataagaat atatacataa
    23821 tgtaataatg ttcttaaa
//
LOCUS       DAEMHZ010000051        23267 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000051 DAEMHZ010000000
VERSION     DAEMHZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.051"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            444..1514
                     /locus_tag="KPK72_001924"
     CDS             444..1514
                     /locus_tag="KPK72_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489141.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFAKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KPK72_001925"
     CDS             1498..2367
                     /locus_tag="KPK72_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2489142.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KPK72_001926"
     CDS             2411..3271
                     /locus_tag="KPK72_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2489143.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KPK72_001927"
     CDS             3302..4606
                     /locus_tag="KPK72_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2489144.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KPK72_001928"
     CDS             4669..5931
                     /locus_tag="KPK72_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG2489145.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KPK72_001929"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KPK72_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG2489146.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KPK72_001930"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KPK72_001930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2489147.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KPK72_001931"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KPK72_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG2489148.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KPK72_001932"
     CDS             13008..13937
                     /locus_tag="KPK72_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG2489149.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="KPK72_001933"
     CDS             14151..15947
                     /locus_tag="KPK72_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG2489150.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKDNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="KPK72_001934"
     CDS             complement(16087..17868)
                     /locus_tag="KPK72_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2489151.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="KPK72_001935"
     CDS             complement(17891..19777)
                     /locus_tag="KPK72_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG2489152.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="KPK72_001936"
     CDS             complement(19794..20291)
                     /locus_tag="KPK72_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG2489153.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="KPK72_001937"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="KPK72_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2489154.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            22408..23178
                     /locus_tag="KPK72_001938"
     CDS             22408..23178
                     /locus_tag="KPK72_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2489155.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         8914 a         3092 c         3903 g         7358 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctgacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgctaaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aatcaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc taggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacgccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagaaaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atatgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agccagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggtgta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtggatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacatac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga attgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggcttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg cgttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagagag
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaaca ctcagtaata
    14701 taattggtaa agagcaaaaa gataattcag atagtagtat agaatcgttt atagagtatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtttaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctcttgctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tgtttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgccc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatactttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgagcgaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcgtttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgatagc
    16681 agcttctttt atcatcttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagccacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgttctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 ttttcctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttagaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aatcctctct ctgtctcaac
    17701 catacaaaca cgacaagaag cacaagtgtc aagcttatct atttcattca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccac
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacctttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatctatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagat gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccttcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgcaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac caaaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatattg gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtatagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa gtcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcttcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct ccttcaaata tttattaaaa taagtaatta tatataataa
    22141 cacaaacata tatctattat atataatacc tcaataatta aaaagtaaaa taaataatca
    22201 aaaggtaaat ttttcaaatt caatataaat tatttcgaaa ttaacaaagt tgttatataa
    22261 taaatagtat aattatatga gacaagtata tataagtata tagtatattt tttatgttaa
    22321 taaaatttat tatatatcat attgttatta tataattatt gccataaaaa gacaaggaaa
    22381 agtataattt ttaaggggga aggatttatg agtaaaggat tcttgacacc tggggaaact
    22441 gcacaagcaa ctataagtgc aggcattaaa aaagcaaata tttcgacaca aaatgcaatt
    22501 ctattaggtt tatttggagg agcatttata ggttttgggg cattggggag tatgatagtt
    22561 agtcaaactt ttggcaagat agacccaggg gtagctagct ttttaagtgc tatggtcttt
    22621 ccagtaggtc ttatgcttgt agtagtggca ggagcagaat tattcactgg aaacacatta
    22681 atggctattg cacttatgga caaaaaaata acattaggaa aaatgttaag aaactgggga
    22741 ctggtttatt ttgcaaattt agtaggttca attttgctag ttgcacttgt gttttattct
    22801 tctacattga caggagatgc agctacaaaa gctatagctg tggcagaagc taaggttaat
    22861 tcaactatag catcaatgtt tttaaaagca atactttgta atattctagt cgttttagct
    22921 gtatggatgg ctacagcatc tcaagatata atgtcaaaat tggcttcatg ttggacagta
    22981 ataatgctat ttgtattatg tggattccaa catagtgttg caaacatgtt ttttatacca
    23041 atgggaatgg cattaggagc aaatataact atggttcaat ttataactaa tttggtattt
    23101 gtaacacttg gaaatatcgt tggaggttcc atagtagttg gagggattta ttatctttgc
    23161 tatgtcaaaa atagttagat aaataatacg aattaaattg ccttttaaag cattgatatc
    23221 agtgatattg atgctttttt taaattattt atattctttt atatgtt
//
LOCUS       DAEMHZ010000052        22904 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000052 DAEMHZ010000000
VERSION     DAEMHZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.052"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(58..666)
                     /locus_tag="KPK72_001939"
     CDS             complement(58..666)
                     /locus_tag="KPK72_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG2489156.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(775..3513)
                     /locus_tag="KPK72_001940"
     CDS             complement(775..3513)
                     /locus_tag="KPK72_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG2489157.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3661..4452)
                     /locus_tag="KPK72_001941"
     CDS             complement(3661..4452)
                     /locus_tag="KPK72_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2489158.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4488..5144)
                     /locus_tag="KPK72_001942"
     CDS             complement(4488..5144)
                     /locus_tag="KPK72_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489159.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5137..6102)
                     /locus_tag="KPK72_001943"
     CDS             complement(5137..6102)
                     /locus_tag="KPK72_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG2489160.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEVKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6208..6314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6604..7548)
                     /locus_tag="KPK72_001944"
     CDS             complement(6604..7548)
                     /locus_tag="KPK72_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2489161.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNVVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKCCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7659..8126
                     /locus_tag="KPK72_001945"
     CDS             7659..8126
                     /locus_tag="KPK72_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489162.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8296..8895)
                     /locus_tag="KPK72_001946"
     CDS             complement(8296..8895)
                     /locus_tag="KPK72_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489163.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9141..9737
                     /locus_tag="KPK72_001947"
     CDS             9141..9737
                     /locus_tag="KPK72_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG2489164.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9835..10794)
                     /locus_tag="KPK72_001948"
     CDS             complement(9835..10794)
                     /locus_tag="KPK72_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2489165.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10828..11559)
                     /locus_tag="KPK72_001949"
     CDS             complement(10828..11559)
                     /locus_tag="KPK72_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489166.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11552..12322)
                     /locus_tag="KPK72_001950"
     CDS             complement(11552..12322)
                     /locus_tag="KPK72_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489167.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12407..13117)
                     /locus_tag="KPK72_001951"
     CDS             complement(12407..13117)
                     /locus_tag="KPK72_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2489168.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13297..13449)
                     /locus_tag="KPK72_001952"
     CDS             complement(13297..13449)
                     /locus_tag="KPK72_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG2489169.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13475..15631)
                     /gene="feoB"
                     /locus_tag="KPK72_001953"
     CDS             complement(13475..15631)
                     /gene="feoB"
                     /locus_tag="KPK72_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2489170.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15731..15952)
                     /locus_tag="KPK72_001954"
     CDS             complement(15731..15952)
                     /locus_tag="KPK72_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2489171.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(15965..16177)
                     /locus_tag="KPK72_001955"
     CDS             complement(15965..16177)
                     /locus_tag="KPK72_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2489172.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16498..19395)
                     /locus_tag="KPK72_001956"
     CDS             complement(16498..19395)
                     /locus_tag="KPK72_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2489173.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(19722..20330)
                     /locus_tag="KPK72_001957"
     CDS             complement(19722..20330)
                     /locus_tag="KPK72_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG2489174.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(20590..21135)
                     /locus_tag="KPK72_001958"
     CDS             complement(20590..21135)
                     /locus_tag="KPK72_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2489175.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(21253..>22904)
                     /locus_tag="KPK72_001959"
     CDS             complement(21253..>22904)
                     /locus_tag="KPK72_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489176.1"
                     /translation="YTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDLSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDISSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
BASE COUNT         7543 a         3972 c         2276 g         9113 t
ORIGIN      
        1 aagatacata cctataaaat accttcctat agatacatat cttacctata taaaaactta
       61 ttctatataa aaacgccata aaaaatcttt tgcttcttct gcataatcta tatttatacg
      121 tttactagtt tttatatcta actcatcttt actaaatact ttttttcctt tatcatcata
      181 ataaaaatca cttatatata attcttcacc cattatactc ttactattta gacttctatc
      241 tatttttaaa gctttgcaaa gttttcctgg tccgttagta atatttttta cttgatattt
      301 actcaactca gtgtaagctt tgttgtatct attcatagat atctcatcta aagaagttat
      361 aggctctacc cctcttatta atacacactc tggcacattt tctatatttg cagataaatt
      421 caagcaatta tacataccat aaattaaata aacatatata tgtccaccat ctaaatataa
      481 tggcttagtt ctatctgtct ttttattgcc atatacatgt gctcctttat catttacacc
      541 catgtatgct tctgtctcta tgattttggt aacaattact ttattttcat atttccttat
      601 tagatattta cctaatattg atttggctaa atcaattcca ttttttctaa aaaaatcctt
      661 ttccaaaatt tcacctcata aaatttattt aaaacgcatc aaatacaact aaagtaataa
      721 ataaaattaa ctcttattaa tgtgatatat ataaacaata aatttagaca tttattattt
      781 atatatatca aaaaattcta tctcaaactt ttcaatatta tcattgtaat ttgaattatc
      841 atatacttct tcaacaacct ctttaattgg gaatataact tttacattag ttccttctcc
      901 taccttacta tctatgtaaa tcttcccatt ttgaatttca atcaatgatt ttgtaaggca
      961 taaacctata ccacttccaa attcatttga tataccactg ttttcaattt gtttaaatcg
     1021 attaaatata tcatttaatt tttctttgtc tatgcccacc ccagtatctt ttataattat
     1081 ctctactgtt tcgtcttttt tatatatacc tacaaggatt tctcctcctt ctaaagtaaa
     1141 ttttatagca tttgataata tatttagtat tattctttct atcgattctg ggtcaagccc
     1201 taccataaca gtttcttcat ctgtatcaaa aattaaatct attcctttag acttagcata
     1261 actactaata gatgtaacta ttctttctgt tatgtcaata atatcaaaat taactgcttt
     1321 aatcttatat atgtctgact taacttgaga aatatttatt atattatcta ttaatctcaa
     1381 taatctaaac atattttgtt tagttatttt tatatgttct tttagtatat cctgaaaatc
     1441 aattattttt ccttttttat aaaggttttc aataagttga actgaactat atatattatt
     1501 taaaggtgtc ttaagttcat gagatatatt agcataaaac tccatcttca tttggtcata
     1561 ctgtctttgt tttattaatg cttttttaag tctttcagac tctctttgca cacttaaatc
     1621 tttaataatt ctcactctac ttcttttctg tccaaagtat aatgttcttg atatcatttt
     1681 tacttcttta atgtgattat tttcatttac tatatggata ctcttctctc tttctcctat
     1741 atttaaattg tttatgtatt tttttactaa tatatttcta tatttttgtg ttgtaactcc
     1801 taatacatca ctagaaattc cgaacaactc tctaaaggct ctatttgtat atagtactct
     1861 tgatgtactt aaatcctcaa gaaaaatagc ctctggtata acatcaagta atttttcata
     1921 tattattttt tgttcattta ctgttctaag aatatttatc tctttagtta tatctctaaa
     1981 tgtaagcaat ttaaagagtt tattatctga ccaatagtct ccaactctaa cttcttcaac
     2041 atgttcttca ccttctgagt cagacatcac aaaattaact atacagtttg ctgtgtcatt
     2101 gtaacttact atttcagctt tcttttcttg tgttagattt aatcttttaa actccctatt
     2161 gtcaaagaaa tctatgttaa aagtttcaaa atattgttct aatgtcatat cgactaaatc
     2221 acttgaaaca catttaaaac tcttttcata ttttttattt atacttatta ccttattctt
     2281 ctttatacca ccctcagcac ttcttacaag tataggagat ggaatattta gtataatgtt
     2341 ttcatagtct ttatttatat ttcttatttc ttcatctatt ttgatttcat ttgtaatatc
     2401 atttaaactt attaagtaat tgctttcatc tttattatta cttaaactta acaaataaat
     2461 tttatcattt aactctacca gtttatcaat tatacttata tcgttatcaa atttcttcaa
     2521 tttttcagca actttttcat cttcctttat aaaagttaat attttataat aattattcat
     2581 atcctttgaa aattcatctt taaatttatt atttgtgtaa cttatattaa aatcatagtc
     2641 tgttactatc catacatttg gtattgtatc cagagcttgt ctaagtaatt cttttctctg
     2701 gtctatgtag ttttttaatg ttctttgtat aatcatatcc ttttctattg ctaaatttat
     2761 ctttcctaat tgctctgact ttttacttaa ttttttattg attgaatcaa gttcataata
     2821 tggatacact actctattat taagactgta tacatataca taataagtat ggttacataa
     2881 tagtaaatat attactccta ttaatctatc tgaatctact atattaatct tagaaattgc
     2941 tacaaaacat ataatagttg taaaaaaaac aaaatatgtt aaatctttta ttaacttata
     3001 gtgctcaatg ttactatcaa gtttattcaa caaatatagt aaattaaaca tagatataca
     3061 aatattaatt aatataataa tcatattcca gttaatatca ctaaaaatat ttcttataaa
     3121 gtaatctaat atcacaaaag cactataaaa aattagtatt gggcttattc ttagtttttt
     3181 tactttagaa atggttaagt atataaaagt agacatatta attatgctta agttaagcaa
     3241 tacaatttgg aatttttgaa tttttaaatt tactactcct aaaatactta caaaaactaa
     3301 tacaagacac cctactttca atatatttat aaattcatct gttttaaagc tacttatata
     3361 aactatagaa tatccaaata tccaaagtaa aattattcta tacatattta tagaaactgt
     3421 atacaaattt atatttaaaa gtgcaataac caaaaataac ccattagcta gtatatagac
     3481 cttattctta cttaaagttc ctttagattc catattcccc accacttctt catttttata
     3541 tagttttacc tataaatatg ttaccacaat tcttccaaaa aatttgtcac tttctagtta
     3601 taaaaaaaaa tgagtaaata tttttaattt tactcatttt gtagattttg tatattcagt
     3661 ttaaaaagct ggaactatac ttcctttata ttcatcttgt ataaattttt taacttcttt
     3721 actattcata gcctctgata aagccttaat cttatcacta tctttattat tttctctaca
     3781 agctattata tttacatatg gagaatcact tgattctatt actattgcat ccttagtagg
     3841 atttaagtta gctgttaaag cataattaga atttattact gctccgtcaa catcttttaa
     3901 tactgttggt aattgctctg cattcatttc ttttattact atatttttag gatttttagt
     3961 tatatctttt ttagttataa gctctccatc ttttacttct atcaatttat tttttgctaa
     4021 taatttaagt gctctagctc cattagttgc atcatttggc acagctataa cagctccatc
     4081 ttttaattca tctaatgact taactttttc tgaatagaat cccattggct ctatatgaac
     4141 tttagatgta taagtaagtt tatatccttt ctccttcaca gtttcctcta aaaatggaat
     4201 atgttgaaag aagttagcat ccaaagaccc ttcatctaaa gctgtatttg gaagtacata
     4261 atcatcaaat atttttactt ctaaatcata tcctttttct tttaacaaag gttttgcaac
     4321 ttccaatatc tttgcatgtg gatttgatga agctcctact agtatttttt tatcctcttt
     4381 gtttgaacaa cctactgctg atattgctat tgctgacacc aatgctactg ataataattt
     4441 ctttaatttc atttttactc cccctcttta aaatgtttat aattaattta ttttagtttc
     4501 ttataagcca gatttcctac tccctggatt atctgaacta atattataag tgctattaca
     4561 gtatatacca ttactgctgt atcaaatctt tgatatccat aaataagtgc tatattacct
     4621 aatcctccag ctcctactgc acctgccata gctgtataac caagtataga tattattgat
     4681 aatgtaattc cagatactat tgaaggcata gcttccttta tcataacttt aaatactatt
     4741 tgtctttttg tagctccaaa agattttgct gcttctatca atcctttatc aacttcattt
     4801 aatgagcttt ctataattct agcgataaac ggtgctgccc caatagttat tggcactatt
     4861 gcagcagttt cccctatact tgttccaacg attaatctag ttattggaat cattgcaact
     4921 ataagtatca taaatgggaa acttctaaat atatttacta taaatcctaa tgcactattt
     4981 attgtgctat ttggttttaa tccatctggt tttgtaacta ctaatatgat tgctaatata
     5041 aatcctaata ttgttgccac tatagttggt actataacca tatacaaagt ttgtaacaat
     5101 gcatttggaa atagtgttgt taaaaagtca attaaactat tcatttatca tttcctccca
     5161 tcttatgccc ttactagtta gatattgttt tacttgctct ccacttttat ctgaaatgtt
     5221 tataataagt gaacctaaaa tatcatcttt aaattgttct aatttcccaa atattataga
     5281 cacatctata tttagttctc ttgccatagt cgtaataata gcttcattag aaatatcttt
     5341 agggaatagt atctttatat ttgttccttt tggcaatatt atactttctt cacctattaa
     5401 ttttctaagt cctttagtat ttcttaagaa tatttcttct gtatctccta cctctaatac
     5461 ttctccattt tccattattg ctactcttcc acaaatctgc tttataactt ccatttgatg
     5521 agttacaact attattgtta ttcccaattt tttatttata tcttcaagca atgaaagaat
     5581 tgactttgta gtatttgggt ctaatgctga agtcgcttca tcacaaagaa gtacttgtgg
     5641 atttagtgct aaagctctag caattgcaac cctttgtttt tgtccgccac ttagatttct
     5701 gggtttatca tttttctttt cagatattcc aactacttct aataactcta aaactctttt
     5761 tttaacttct gcctttgagt atccaaaaca ttctaatggc aatgctacat tatcaaatac
     5821 agtttttctt tcaagcaatg aaaagtgttg aaaaatcatt cctaactctt ttcttaaatc
     5881 tctcatttgc ttttcattca atgactttac ttctttatca gaaacaagta cactgccttc
     5941 ttggtattct tctaatccgt ttatacatct cagtagtgtt gatttccctg caccactatg
     6001 acctattatt ccaaaaattt ctccagattc tatttcaata cttacatctt taagcacttg
     6061 aatctttcca taatacttat taacattttt tatgctaatc ataaaatttc ctcccatata
     6121 ttctgctcag tttttatgtt tatcctctct tatgtattac aacaaaataa aagcccgagt
     6181 ataaactcag gcttagaaag ctatttatac tcatctatca actatattag ttgcaggatt
     6241 tagcaccaat gtatataaaa tatactggtt gccgggtttc aaagggccag tccctccacc
     6301 actcttgata agacgttatt taatttaatt ctattatcat ttgttgctat gttatatatg
     6361 atatattcat taatagtatt tgtcaataaa tttatttaaa ttttttattt tttattatcg
     6421 ttaattaact atttttaatt agttttctag ctcctaacac tttatatttt aatgtctata
     6481 taactataga acatcattat aagaaataaa ttaaataaca tcaatctatc taaatcatct
     6541 gtaaagtaaa ttaagaaaat taacatttta tccaaactct atcctactat taaataaaat
     6601 aatttaaata caatgatgat tatatagctc tagtacttta gactttatca aatgtttaag
     6661 ttctataggt tctattattg tagcattttt cccaaaagtt aaaaaatatt cagccaccca
     6721 gttaagtgta gattttggta tatacatatc tatgtatcca cctttagaca agattttcaa
     6781 tcctcttgcc aacaaaaatt ctgtttcaca acattttact ccctcatctg tcaaattaat
     6841 tttaatatga taatctgtac ctacttccat attcttaaga taatcatgta tagaagataa
     6901 aaccttatat ttcttacttg ataagttttc tatactcaaa atcttcacaa ttctgtctac
     6961 acgaaattcc ttaatagatt ctgattttat acagtaagct ggacaatacc aaagtccatt
     7021 catggcatac aaaccaatag gaacaatggt acgtacacta tttttttgct ttgatgaata
     7081 ttcaatttta attgtagatt cattcattac tacattaaac aattctttca ataatggtgt
     7141 tgaacagtgc ctatctggtg tccaaaaaac aaatttacgc cttatattct caatattata
     7201 ttgaatatca tttggtatac aatttaaaaa tttttttaaa acagaaatag tttcttgctc
     7261 aaatggcaaa tcattataat attctagtga ctgatatgca aagaatatag attttacctc
     7321 actttctgaa aatgcaatcg gtggaagtat acgctcttta agtacatgat atccaccagc
     7381 cgctccaact tttgaatata atggaaatcc aatactctcc agcacctgta agtctcttag
     7441 tatagtacgt tttgatacag aaaattcttc tgctaattct cctgctgtaa aacttctttt
     7501 tgcatttatt gttatcatca gttcaattaa acgctcagct cttgacatat ttatactcct
     7561 aatttaaata tagtgacaat aattgtcact attatatgat aagatattct tacatttaaa
     7621 gcaataataa tagtaaagaa agaagggact gatactttat ggaaaataat actattaagg
     7681 ctgatgatat tatcaattat tgtctatcaa acttagatga tgttttatta atgaatagtt
     7741 ggggagaacg agcaatttat tacaatccta atggtatttt aaagcgagga gtatatgttc
     7801 ttaccattaa agaaaaagac agtaatcatg ataaaggttc gttggttagt cgtccaaatg
     7861 tataccgtgt gaatatagga ttaaaaaaag aaacttttat tgaaatgttt ggatatattc
     7921 caaagcgtcc aggtgtaggt caaatagttg atatggattt tgattttaca aaattggaca
     7981 caatcatgcc acatcctatc tactcatgga tgggatggat atgcgtccta agccctactg
     8041 aaaagacatt tgagaacttt aaaactttaa taggagaatc ttacaatttt gcaaaacaaa
     8101 aatttaaaaa gagaaaaaac aagtaaaaac gtgtgaaaat aacctctaaa taaattataa
     8161 taaaaatatt aaggttacaa gataaaaatt aatatcttaa taacatgcaa aaaagcgaag
     8221 ccaaaaaatg tattatttat aaggcttcgc tatatctcaa ataatttatt tcaaatttca
     8281 aaactattta ttctattatt tcttattatt tcctgttatt cctaatctat cattcatatc
     8341 ttttatcatt tttctaaaac cactacctat atcatttcca acatcttcag ttccatgaac
     8401 taattttata aagtcatttt gacctttttt catatcttca tcagatacct tatctaattt
     8461 ttctattctc ttatctatat cttttactaa tctttcccct tcagcgatta ggtcatcatt
     8521 cattttcttt aactctgtat cttctgtttt accatctttt ttaagagatg ttacaaatga
     8581 ttgtatttta tctctactat ctttatatgc agcctttaca tcacttagat atttttcatt
     8641 tcctggatat tctttatctt taaactgatt atttatactt tctggagtat cataaataga
     8701 atatctattt agacctgcta tataatcatt atagcttgtt gaataataat tcatatatcc
     8761 ttcattatta tttacagatt cattaggatt ttttcctgta ttttcacttg gtttttcatt
     8821 atttttagta catgcagtta tacctatagc taatactagt gctaatccta caaataaagt
     8881 tctttttaat ttcattttta ttaccctcct agatgtttta tactataaaa aattttaata
     8941 attttctttc ttaatttagt atttctatat ttttatattt tatttactaa aattaaacaa
     9001 gttataattt atttttttgg aaatcataat gaataaataa ttaaataaaa aaattataat
     9061 tttattattg atattgatta tcattcatag tataatctaa ttacttgata ttaattttca
     9121 tttgaaggag gttatctttt atgtcttgtt gtaattcaca attttgttgt aaaaataaaa
     9181 ttgaatcatc atatataaaa tggcttttag aagttttagg tccagtcttt ttaggctcta
     9241 aacctgctga aataataaat atttcgttta ctgatgtaaa tagagaggaa aaaattaatg
     9301 atatttataa atatctatat aaatgtaaga aaatagattt tatagttatt gataaagaaa
     9361 aaaagggatt aaaaatctta tttgtaaata aaaaagcatt atcagaaaaa ttaaaatgta
     9421 aaaaaacagt aaacttttta aagtttttag gatacaaaca aaatacgaat gttaatgctt
     9481 atttagagca tttagtagat aaacttaaga gtgatgtttt tcctgatgaa ataggtattt
     9541 ttttaggata tcctcttaaa gatgtgattg gatttatggg atatagtaat tatgaagttt
     9601 ctatgattaa atattggaaa gtttatggtg acacaaaaca atccgaggac acatattcga
     9661 agtttttact tcaccgtaag aaaatgagaa aattactaga ctacataagt gtagataaaa
     9721 tagtaagctg tttttaattg tgtttttgta taataataaa aatacaccct cttttaccat
     9781 acagtttcca taataaataa gggtgtattt tccactttaa tattttaaag tattctaatt
     9841 ttgattgtac aacatatctt taatatttat tttcttttgt tgcattccat tagacatcaa
     9901 aaattcctgt gtatcttcta attcttttat atccttgtcc gttattttag aactaaagtc
     9961 ataccaagaa tacatttctt ttgtttcatc taaagataaa ccaacttctt tagatacaac
    10021 atccatcact tcatccttgt tttcattcat atattttaaa gtttcttcat gaaccttcat
    10081 aaatcttttt actaattcag gatatttttc agcaaagtca tcacttacag cagtaactat
    10141 tattccactt actaatcctt ctccatttac aatcattttg gcacctgaat ttatcgtttt
    10201 caatgccact ggacctgcaa gcattgctac atcagcactt ttactttcta atgcagaagc
    10261 agcactagga atatccatat ttacatattc aacatcatct atagtatagc cttctttagc
    10321 aagtgcagca atcaacagtt gatgaagtat agtccctttt ggacctacaa tttttttacc
    10381 ttttaagtct tttatagatt taatattatc attattagtt ataagcatat atccttttgg
    10441 tgaacggcta taaatattcg taattttaag accaacacca tttgatgcag ctattattgc
    10501 agacgttcct cctaatgcat gtaaaaagtc taactcacct gctgctaatg cttgagtttg
    10561 ctctggtcct gttgtcaatt catggaagtt gacttttata ttgtcctttt taaattcctt
    10621 tccaaataaa tcatcctttt tttctaatat actaggaaca ttaagtggtg attttacata
    10681 agttaaattt atttccttag gtaaactagc ctcttttttt gcaggttcat ctttcttatc
    10741 atttgttgtt gaacatccta cagtcccaac caacaccatt attaaagatg tcattactgc
    10801 taaaatcttt ttgtttcttt taatcaatta aaactccccc aattccctta atatttcttt
    10861 tttcaagttt atataatagt ttttagttaa atctctatta tattcatctt cgacactaaa
    10921 ttgtttaatc cttttatttt tactaaaaac aattattttc tttgctattt ccatagcttc
    10981 ttcaatattg tgagttacaa atactacacc ttttttagta tttttatgga tatttacaac
    11041 ttctttttgc atttttcttc ttgtgaagta atctagtgct gcaaatggtt cgtccatcag
    11101 caaaatatcg ggattgaagg aaagtgctct tgctatactt actctgtgag ccattcctcc
    11161 tgataattca tttggatatg agtttttaaa cttttcaagc ttcatcattt ttaaatactt
    11221 gtctaaatca actttatttc tattatcttt ttcagtatgt aaaagtatat tttcacttac
    11281 attcagccat ggcataagtc tactttcttg aaataccatt cctacttttg gagtacactt
    11341 tttatcatta ttaaaaaaat atacgtttcc actagtagca ttttcaagcc ctgctaaaat
    11401 tctaagtagc gttgttttcc cacatccact ttcacctaat ataacagtta tctcttcact
    11461 agatatattt aaagatatat tatcaagaac taaatgctct ttattatcaa ctaaatattt
    11521 cttatatata ttctctagca aaaatccact tctaaccatt taaatcaacc tgcttccctt
    11581 tgctaaattt tgttattatc tttgaaaata gataatctga aagtatacct aaaaatccaa
    11641 taacaaaaat tcctaccaat acaacatcag ttctagacat atcttttcca tcagaaatca
    11701 aatatcctag tcctgaagaa gctgcaataa gctctgctcc aattatagcc ctaaaactat
    11761 acccaagccc taaactaagt ccaacaacaa tatccagcat agaattaggg attattattt
    11821 tataaaaaat tctcttttta gataaattaa aagatttacc tacttctatt aatttattgt
    11881 cacaattttt tatacctttc aatgtattta aaaatattgg aaaaaaggat gccaaaataa
    11941 taattataat tttagattct tcacctatac caaaccataa aataagcatt ggcaccaaag
    12001 ctagtggagg tgtacttcgc ataaattcaa atagtggttt gaaatattca tacatttttt
    12061 tatttactcc aaataaaact ccaagtggta tagccatcaa tgaacttatt aaaaaaccta
    12121 ttaatatcct tcttacactg gcataaacat ttaagaaaat agaaccgtca tatatcattt
    12181 ttataaaagt atctaagact atttcaggag gaggcagaat atagctactc catataccca
    12241 aaacacatac tatcttccat aaaattaaaa ttattaaaaa aatataatat cctttaagcc
    12301 ttttatgaat tttctcaaac atataaaact ccttttaaca tatctatccc tgctaccctt
    12361 tttatttttt aatatcttaa ataataatta agctatatca taagatttaa tctatgccaa
    12421 gtcttaatcc acctgaactc attctatcag aaggtgtaga atctgttttt aagccagaca
    12481 atctttcaga tggttgtata atgactattt gtccaggttc aacaaattcc atatgataag
    12541 attctcctga agtctggcca ataaaagtct tccatgatag ttttgatact ttaggatatg
    12601 gttctcttcc agtccaagct actaatgcat ctgggtcaac acaacaaggt ccttcaagaa
    12661 ttagaggatt accatctgtt attatagcaa catcttgatt ttgacctgtt ttatttacta
    12721 aatgtgtagt aaaaagacct ttttgagata ttacccctga accaatcaat tttacactgt
    12781 aatttaattg ttcagaaaat gccaataagt tttcactctc aacataaatg gaatcacctg
    12841 ggtcaattga tattacatct acatggtaag cattttgagc taggtaaatt tcaccttcac
    12901 cttcaaccac cattattggc atttgttctc ctgtcaatga cctctgaaca tgacctaata
    12961 atgctcttcc caatccaccg ccattacttg gacctaacag caatttttca aaattaaaat
    13021 ttcctttaaa ggctaccata gcaccttttt ttgcaaaaaa tttaccactt ccccttgcta
    13081 tacacataag ctcgtttgta acatcaaatt taaacataat attcacttat attgcttaat
    13141 ttagcaataa tccctcctaa tcatatattt atatataatt atacagtaaa attacagcat
    13201 agtctaacct acttcaaata tgaaatttgt tatttataca aaaaataaga acctgataat
    13261 aaagcaattt aactttttat caggctctta tcctatttat ctcttagcgc tatgacaact
    13321 cttactactt gagcatccag aacaaccaca tccacaactg ctaccattct tttttgaatt
    13381 tttaaccata tacattacag ctagtgccat taatactact acaatagctg ctataataaa
    13441 tgtagccata ttcatcactc cttatttata tgaattaatt tttcatataa cttaatttac
    13501 ccattttttc ttttttcata tttttatttg aataagttaa aactacaaag actactgcta
    13561 ctgctataaa tatagatatt attgccccag caatacttcc tccagtacct aaaactaaac
    13621 ttccaacttg atttataaga agagcaatta tatacgctgt taaagtttgg aatcctaatg
    13681 taatccatgt ccatttccat gaacccattt ctctttttat ggctccaata gctgcaaaac
    13741 aaggtgcaca aagcatatta aatgccataa atgcaaacgc actagctact gtaaacatgc
    13801 ttgcaactga agcaagaagt gttgggtctt gctctgttgc atctgatata ccaaataaaa
    13861 ctccaaatgt tccaactaca ttttccttag ctactagacc tgtaactgtt gctactgatg
    13921 attgccaatt tccaaatcca agaggagcaa atattggtgc aactatattt cctaagctag
    13981 ctaatatact atctccagca tctaccattt gtaatgacca attaaatgat tgtaagaacc
    14041 agattactcc acaagcaaca aatataattg ttcctgcttt gattataaat gcttttcctc
    14101 tatcccacat atgtattaaa acaccttttg cacttggaat atgatattga ggtaattcca
    14161 taacaaatgg agatggttct cctgcaaata aactagtttt ctttaatata attccacaaa
    14221 tgattatcat tgcaatacct aagaaataca ttgatggagc tacccatgaa gcacctccaa
    14281 ataatgcccc tgcaaataat gctattattg gaatcttagc tccacatgga atgaatgtag
    14341 ttaacataat agtcattttt ctatctctat cattctctat agtacgagtt gacataactc
    14401 ctggaactcc acaacctgaa cttataagca ttggtataaa tgattttcct gaaagaccaa
    14461 acttacggaa aattctatcc attataaaag ctacacgtga catgtaccca caatcttcta
    14521 atattgataa taataagaat aaaagcatga tttgtggtac aaatcctagt actgctccaa
    14581 ctccaccaat tagaccatca actacaagtc cttgtaacca ttctgctaca tttaaagaag
    14641 ctagaaaatt acttacattg ccttgaataa tttctccaaa cagtacatca tttgtccaat
    14701 cagttgctat agtacccaat gaacttactg ctatatagta aactccccac attataagtg
    14761 caaaaatagg taatgctagt atacgatttg ttacaatctt atctatctta tctgatacag
    14821 tttccttacc ttttcgattc ttctttatta tacttgaaat tattgaacta ataaattcat
    14881 aacgattggc tgttattata ctttcactat catcatccaa ttcatcttca caatttctag
    14941 ttatttcttc aatactattc aatattgttt tagaaatatc aagtttttgt ataacatttt
    15001 catctctctc aaatagcttt atagctaacc agcgtgtttc tacatctata tatggagtct
    15061 tttcttctat tattttttca attttttcta ttgcatcctt actttcatca acaaagggta
    15121 atttgaaatt taatttattg ttactacttg caagttcaac tgccttttca gcagcctcca
    15181 taattccttg acctttaact gctgttactt ctaccactgg gcaacctata acttcagata
    15241 gttctttaat atttatctta tcaccattct tatttacaat atccatcata ttaagagcta
    15301 taacggtcgg tatacctagc tctaaaactt gagttgtaag atataaattt ctctctatat
    15361 ttgatgcatc tacaatattt attactgcat ccggcttatc atctagcata aaattacgtg
    15421 taactacttc ttctaatgta tatggagata aagaataaat acctggtaaa tctactattt
    15481 ccacatcttt atttcctttt aattttccac cttttttttc aactgttact cctggccagt
    15541 taccaacata ttgtgatgag cctgtaagcc cattgaacat tgttgtctta ccacagttag
    15601 gatttcctat gagacctatt tttattgaca tttccattcc ctccattatt atttaaatac
    15661 atcactgttt gtgatacaga aaatcattct cattatataa ttacattaac cctactctaa
    15721 ttaatcttta ttattccaca ataatttttt ctgcatctga cttacgaatt gatagttcat
    15781 aatctctgac tgttacctca attgggtcac caagaggagc tacttttctt ataaatatat
    15841 taacacctct tgtaatcccc atatccatta tacgtcttcg tgtagctcct tctccttcta
    15901 actttttgac ttttacagtc tctccacact taatatcctt cagtctgttc atgtcattac
    15961 ctccctatat tattattcta cttgccattt ttttatctaa tgcaactcta gtatctttta
    16021 cgtctattat taagttacca tctatcttag aaactatttt aacatttgta cccactacaa
    16081 aacctatact atttaaaaaa gtcctcgtct tatcacttcc cgaaattttc tttataacta
    16141 cttcttcacc tgtatttgat aatatcaatg gcatcatata atatccctcc tcttaaagta
    16201 atttaaatct acaagcaatc gttatcttat atataaatta caatacctta aatcttaatc
    16261 tattaaaata atatcctatt ttttccttct ttttacaatt cctaattttg atttaagtct
    16321 atcacaatag tatatttttt gcaagtgatt attgtattct tttgattttt tttctcattt
    16381 gatttatttt attaataaaa tcgtaaaaat taatacaaaa ataaagatgc tctaaaatag
    16441 tatactcaac taatattaaa gcacctttat tatatatgtc attatttaca cattgcttta
    16501 ttctttttta aaagcgattt tttcaaactc ttttacaggc attggcttag aaaacaaata
    16561 tccttgtacc atatcacacc caatttgctt tagaaattct acttgcgaaa tagtctctac
    16621 accttctgat attactttca ttttcagttc ctttgccatt ttaatgacat tagatataac
    16681 aattttttca ttattactct catctttttc tgtaaaaaat gctctatcta gttttaatat
    16741 gtcaacaggc aattctttta aaagattaag agaggaatat cctgtaccaa aatcgtccat
    16801 agagattaca aatccctttt ctttcaaatt gttcattgta tcaagtaatc cttctatatt
    16861 atctaatact gctgtttctg ttagctctaa ttctataagt cttgtcggaa ttttgtattt
    16921 atctacaatt aatttaaact tctctataaa attactattt acaaaatgca ttcgagacaa
    16981 gtttacagat ataggtacta ctttttgtcc ttcatccatc cattctctaa tctttttaca
    17041 cacttcctca aatacataga aatcaatatt tacaataaaa ccattcctct caaatacagg
    17101 aataaattct attggtggta ttagaccttt ttgtggatta tcccaacgaa caagagcctc
    17161 tgcccctata atttgataat ctgaaagact atatttaggt tgaaaataaa caataaattc
    17221 tccattatta agagcatcta ccattgaatt ttcaatttct ttctctttta atattttttt
    17281 gtgcatttcc ttatcgtaaa aagcaaatga atttttatga ccacctttta tagtttttct
    17341 tgcagtatta gctctatcca taatagtatt tatgtcatta tcacttttac ttattttata
    17401 tataccacaa tctaaaatca atttataata gtttatttta ggattactaa gtatttgaac
    17461 cttattgtaa atattgttta accttgtttt aatatcatca tctgtcttat acttaataag
    17521 aagaacaaaa tggtcagcag aaactcttgc aaaaatttca tcttccttta attcacttgc
    17581 aatagtattt gatatatgta tcaaagtatc atttcccata tcatatccaa acatatcatt
    17641 tatatattta aatttgtcaa catctatata aaacaacgca tattcttctg gattattttt
    17701 agcaaacagt ttattagaat ttattttaaa tttatctata ctatctgctc ctgtcaatgg
    17761 gtctgtatat gctatcttcc ttagataatc ctctttcttc tttttattat aaattatata
    17821 tttaatagca attaagaata aaatacatac aaaagaaata atttgtattg aatattcttt
    17881 aaaaattaca ccaaatggta ctttataagg tttgtcaata gtattagaat atatacaatc
    17941 acttaattct tcttcagtaa ttttgtctat tgatttattt aaaatagaca ctaatatagg
    18001 gtcagtttta tttgataccc ctatacgcat cccatagctt gtgtctaaaa cagatataac
    18061 agataaattc tcactctttc cttctcttaa aagttcatca aactgataag agctaagtat
    18121 cattaagttt gctttacctt catttatagc gtttagacaa ctttctacac tcttataaga
    18181 tgttgtctta gcattttcaa aactcttttc tatataagaa tcaatagact tataactact
    18241 agttaaagca atatttaaat cattattgag attggtatcc aattttccta ccataatcat
    18301 tggaatattt atgtatggat ttgtcaattt catattaaat cgttttgcct tatcattttc
    18361 attgattgag ccacatagca tagttgcctt accacttttt atgtaatcaa gactctcatt
    18421 ataatttttt gttttaatat attcaaattt tataccacat ttttttgata taattgacat
    18481 taaatcggaa cttatacctt taaatgaatt gctttcttta tcataatact ctacaggaga
    18541 ccactctgaa tcatatacca tagtaagttt tgggtttgct ttaataaaat tcatttcttg
    18601 ccgagtaaat gctatggaat tattgacctc tcttttgaaa tattttttat ataattccaa
    18661 ctcataatac atatcattta tttttaattc ttttaaagca taatctaatt cttttactaa
    18721 attaggttta tcttttgcag tagcaaaata aaaaggttct actgaaaatt tgctaactat
    18781 tttcattcct ttagcattta taatacttcc acaaacaaat gcatctgcct ttttttcatt
    18841 gacggactca attaattcat caatagaatt acagtatata tattcaactg aaaaattatt
    18901 ttcttctcta tacttctcaa atgcagtatt atgaaagttt cctctaagta cagctactct
    18961 cattccctta aaggaactta tatcttcata aaacatatta ttgttactat cctttgtata
    19021 tagtgcacta tattcatatc ctgaacttag tttaggaaaa ttaaatagtt tatttctttc
    19081 atcattcttc tgtaaaggtc cgagtaagtc tatttcaccc ttttctagtc tgtctaaaca
    19141 ttcttcccaa gaccctttta caaattcata ttcccatctt gtatattttg agatttctct
    19201 tagataatca taaccatatc ctgtataaga atgctctttt gatgaggata ttattccgta
    19261 gtcatcacaa tatccaactc ttattttttt tgaattttca gactcatcag caaagcatag
    19321 tatattgtta aaacatgata taataagtaa aaatatcaaa gaaagtatca agaagtgctt
    19381 tttaaatgct ttcatattta aaaacctcca atttgccaac ttactttaac ttatttggtt
    19441 ttctagttgt aactactttt attgtaatgg taaatatatc aaattgcaat attctcatat
    19501 aatattctta ttcaactata atgaatttat tattatctat ttaaatacac tatctaatat
    19561 tagtttctta taagtcataa aagcacaact aacactataa ttctagtgat taattttatt
    19621 tcaaaaaata taataacact actaatattt taaatatcaa tagtgtcata aaaacttttc
    19681 agattaatta aatctttcta tatattttat aataatctaa atcatgctaa atatttagac
    19741 agaaaacttt tttctttaat agtaaatact tttgtttgaa tatcagaata attatttgaa
    19801 tatctatcta taaaaccatg atttgcatct aaaatctcaa tttggatatt ttgtttccta
    19861 gaaagttggt aagaaataga atcaacatca aatgaatcgt gctttgcaaa tactaataag
    19921 gtaggacatt ttggaattac atccatgtaa tctcgaatcc gagagccata acaacaaatt
    19981 atgccatcac aaaaagaatc ttcactgcat ctccatgcaa tagttgctcc cacgctatac
    20041 ccagcaacaa atacatattt atactctgat tttaaatcat ttataagctt ttcaactttt
    20101 ttataaatat caaaaccaac attatttata aaaaaatcat atgcctcttg gacttttgaa
    20161 tatggaaaaa ctcttttttc tcccagaagt tcaggacaaa atacatcata tccatattta
    20221 taatactttt gacaaacatc ttctataaag ctattaatcc catagatttc atgcaaaaca
    20281 acaatagcta catcactttt atgtatatac tttatcagat tatccaccac ctttacaaaa
    20341 cagcaaattt tttatactta gtttaatata ttcttcttta catgtcaatt tacttttcta
    20401 aaatcaaatt tatttttgga ttaatcattt atttttaaaa gttgtgcatt attactatta
    20461 aattattctc tattattaaa aatataatcg ttagaaataa aaaataaagt acaaataatt
    20521 aaaaaatatc tcactcataa ctttaaaaat tataagtgag atactcctaa aatctacttt
    20581 atattaaatt tattttccaa aatatctatt taataatcct aagaatgctt taccatgtct
    20641 agcttcatct ttacacattt catgtactgt atcatgtata gcatctaatc ctaattcttt
    20701 tgctttctta gctaatttta attttccatc agttgcacca tactcagcat caactctaac
    20761 tcttaagttt tcttttgtat ctgcaactac aacttctcca agaagttctg caaatttagc
    20821 agcatgttca gcttcttcaa gagctattct cttataagct tctcctactt ctggataacc
    20881 ttctctatca gcttgtctac tcattgctaa atacattcct acttctgtac actcaccaac
    20941 aaagttagct cttaatcctt cgattatttc ttcatctaca ccttgagcta ctcctattct
    21001 gtgctcatca gcccaatttt tttcgccttt catttcttca aatttatcag ctccaacttt
    21061 acatactgga cattgtgctg gtgcagcatc tccttcatgt atatatccac atactgtaca
    21121 aacaaatttc ttcataatta ctttcctcct aaattttata tatcaaatta aattttttat
    21181 ttattaagtt agtctatata tacccgcacc atataccttt aaacaatttt tttaaaagtt
    21241 ttttctaaat acttattttc cctgtgtcaa taatatgttt ctataataat gtccctttaa
    21301 ttttatcaat tctttatgat ttcctgattc cacaattcta ccattttcta aaactattat
    21361 attatcacaa tttgatatgg tacttaatct atgagctata attatacttg tctttccata
    21421 agttaaatta tttattgctt cttgaatttt agcttctgtt gctatatcta tgttacttgt
    21481 agcctcatcc aaaataagta ttggagcatt ttttaatact gctctagcaa tacttattaa
    21541 ttgtttttga ccttcactta aatcttcacc cctatccgat aatacagtac tatatccttc
    21601 acgcatttta actataaatt tatgtgcatt tgctatttta ctagcatata taacttcttc
    21661 atcagtagcc tccagatttc cataccttat attttccata actgtatcat tgaatatata
    21721 accttcttgt ggaactactc caaaacaagt tctcaaatag tttctgttaa tagaggatat
    21781 atccttacca tctaaaaata tacttccttc atctatatga taaaaatttg taattagatt
    21841 tataattgtt gtttttccac ttcccgtctc tcctatgatg gcatttgatg tgccagcttt
    21901 gatttttagg tttatattat ctaaaatttt cttttttgaa tcataagaaa aacttaaatc
    21961 tttaagctca atttctccct ttacaaaatg agagtttaaa ttaccatctt tattatcgtt
    22021 aatctcactt ttaaacatgt tatgcacttc ttgctcttct tgttcatcta taatttcaaa
    22081 aactctttca catccagcta tggctgaaag taatgtatta aatagatttg ctaattcatt
    22141 taaaggtctt gtaaattgtc ttacataact taaaaaacta gctataaccc ctatggttat
    22201 aatatcttta gatgcaagaa tgcctcctat aatacatata gttgcaaatc ctatattact
    22261 aatcacattc ataataggca ttaaaagaga agtgtaaatt tgcgatttaa gggaaatatt
    22321 tagaagtcta tcattgactt ctttaaaatc atttacagat tttctttcat aattaaaaga
    22381 ttttactaca tttatgtttg atatcatttc ttcaatatgt gcatttagtt ttcccaactc
    22441 aacttgttgt tgcttaaaaa aaactctagt tctctttgca ataaaattac tcacaaacat
    22501 tactatcggt attaatatta cactgcaaaa ggttaaaatt gggcttaata caaacatcat
    22561 aataaaagtc atcaatatgt ttaagattcc tgtaattaac tgtactaatg aatttgatat
    22621 tcctgtactg atattatcaa catcatttgt cactctactc ataatatctc cagtagaact
    22681 tttatcaaaa aaggttattg gtagattttt aaattttaaa aataaattac ttctcatttc
    22741 tttaactata ctttgagata atctagctgt acatatcccc ttagacagtt taactgaaaa
    22801 ctcaattata taaaatgaaa gtaaaataat tattcctttt aagaaaatgg attttgaata
    22861 agtatcgata tctattatgt caactacctt tccaatagta tatg
//
LOCUS       DAEMHZ010000053        22638 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000053 DAEMHZ010000000
VERSION     DAEMHZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.053"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1842)
                     /locus_tag="KPK72_001960"
     CDS             complement(<1..1842)
                     /locus_tag="KPK72_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489177.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIDKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENDNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIF"
     gene            complement(2025..3500)
                     /gene="spoIVA"
                     /locus_tag="KPK72_001961"
     CDS             complement(2025..3500)
                     /gene="spoIVA"
                     /locus_tag="KPK72_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG2489178.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3818..4837)
                     /locus_tag="KPK72_001962"
     CDS             complement(3818..4837)
                     /locus_tag="KPK72_001962"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2489179.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(4868..5482)
                     /gene="plsY"
                     /locus_tag="KPK72_001963"
     CDS             complement(4868..5482)
                     /gene="plsY"
                     /locus_tag="KPK72_001963"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG2489180.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(5517..6836)
                     /gene="der"
                     /locus_tag="KPK72_001964"
     CDS             complement(5517..6836)
                     /gene="der"
                     /locus_tag="KPK72_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG2489181.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(6870..8198)
                     /locus_tag="KPK72_001965"
     CDS             complement(6870..8198)
                     /locus_tag="KPK72_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG2489182.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(8436..8636)
                     /locus_tag="KPK72_001966"
     CDS             complement(8436..8636)
                     /locus_tag="KPK72_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489183.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(8638..9570)
                     /locus_tag="KPK72_001967"
     CDS             complement(8638..9570)
                     /locus_tag="KPK72_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2489184.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(9615..10547)
                     /locus_tag="KPK72_001968"
     CDS             complement(9615..10547)
                     /locus_tag="KPK72_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2489185.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(10602..11891)
                     /locus_tag="KPK72_001969"
     CDS             complement(10602..11891)
                     /locus_tag="KPK72_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2489186.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(11920..12612)
                     /locus_tag="KPK72_001970"
     CDS             complement(11920..12612)
                     /locus_tag="KPK72_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489187.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(12627..13388)
                     /gene="pgeF"
                     /locus_tag="KPK72_001971"
     CDS             complement(12627..13388)
                     /gene="pgeF"
                     /locus_tag="KPK72_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG2489188.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(13385..13846)
                     /gene="nrdR"
                     /locus_tag="KPK72_001972"
     CDS             complement(13385..13846)
                     /gene="nrdR"
                     /locus_tag="KPK72_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG2489189.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(13868..14131)
                     /locus_tag="KPK72_001973"
     CDS             complement(13868..14131)
                     /locus_tag="KPK72_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2489190.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(14235..15008)
                     /gene="sigG"
                     /locus_tag="KPK72_001974"
     CDS             complement(14235..15008)
                     /gene="sigG"
                     /locus_tag="KPK72_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG2489191.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(15100..15822)
                     /gene="sigE"
                     /locus_tag="KPK72_001975"
     CDS             complement(15100..15822)
                     /gene="sigE"
                     /locus_tag="KPK72_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG2489192.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(15847..16719)
                     /locus_tag="KPK72_001976"
     CDS             complement(15847..16719)
                     /locus_tag="KPK72_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG2489193.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     KIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(17009..18283)
                     /locus_tag="KPK72_001977"
     CDS             complement(17009..18283)
                     /locus_tag="KPK72_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2489194.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(18567..19724)
                     /gene="ftsZ"
                     /locus_tag="KPK72_001978"
     CDS             complement(18567..19724)
                     /gene="ftsZ"
                     /locus_tag="KPK72_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG2489195.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(19914..20267)
                     /locus_tag="KPK72_001979"
     CDS             complement(19914..20267)
                     /locus_tag="KPK72_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG2489196.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(20366..21040)
                     /locus_tag="KPK72_001980"
     CDS             complement(20366..21040)
                     /locus_tag="KPK72_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2489197.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(21045..21743)
                     /locus_tag="KPK72_001981"
     CDS             complement(21045..21743)
                     /locus_tag="KPK72_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2489198.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(21766..22506)
                     /locus_tag="KPK72_001982"
     CDS             complement(21766..22506)
                     /locus_tag="KPK72_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG2489199.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         6832 a         4263 c         2220 g         9323 t
ORIGIN      
        1 aaaaataata tcttttttta gattatattc tttagattct aatttataat tattatttat
       61 tattccatca taatcactta tatttgactt atttttattt gtatcttgat ttttgttaga
      121 aatactttcc ctataaggaa aagaatgttc attggtacca aatactgatt ttgagcttaa
      181 atcataatta tcatcatttt ctgtaattga atttttattg taaaatcctt tttctttatc
      241 agaattattt tctttattac catctttagg tatatcatga ggatttttat tttcattgct
      301 atcagtactt gattctacat catctttagg agtatttggt tgtttctcat cagaattatc
      361 attttcatta ttattgtttt gattgttatt actatcatta ttttcatttc cattattccc
      421 atcttgattg ttatcatcct catctctatc cgggtcgtct ctacctcctc ctccacggtc
      481 accatcaata tcatcatttt cccgttcatc atctggtgtc tcttggttgt ctccgcctcc
      541 cattataaat cctggtttaa aaactatatt tgaataccct tcttttaatc ctcctacaac
      601 ttttactcta aaacaacgca acttaaaatc ttcaaaaaaa agtatagctt gatctctaat
      661 atcctcctgt tcttcagata tccaattgat tccatcgctt gaatattcaa caaaaacctt
      721 acttgcacta tacggcatat cataaaataa aacagctaca tatccatttt ttgtattttg
      781 aaattccagt tccaaattgt ctattggttt tttatctata acagatacat tcaaaatagg
      841 aactacatca tcatttatta taattttttc tttatttaac atttctgttt taaactttcc
      901 tgttatatta aaattttctc ccttttccaa cttttcataa taatcagtaa aatcccattc
      961 tacatctata tcaagtcgat taccactaga tgtttctaat accatatatt tgggaaattt
     1021 tatattttct attttatcat ctttacttac ttttatttca tcactaaata caccaattcc
     1081 cctatgacaa catttcacaa tattgtagcc agaatcttca gctatagaag gtataagctc
     1141 attataatca tcaattatat aaactttctt actttctgat ataattgatt gtgccaactt
     1201 attattatct tttagtaacc cactttcatt gttgctacta tatgctttta ttgaagtttc
     1261 ttctatctca acatcacctt tagaataaac acctatagca tgttctccat tcacttcaat
     1321 atccttattt agtattctaa tatcattttc agtataaatt cctattgcat ctttcccatt
     1381 tgcctttatt ttttcaacag attcgtagga tgatttaata tgtaagtctc ctccttcttc
     1441 aacgtataag gctgtcccat cagtagcaat tatgttgttt ataccaatac ttatacttcc
     1501 accattttct atatgtatta ctccttcttt tcctccttca cctattatat tcaattcatt
     1561 atcatcaata ataaatctcc catgattttt tatcaatatt ttatatttgt tggtatctat
     1621 tgtaaaatta ctgtcataag gaatataaaa tctaaactct tcatctatta ccatatcaca
     1681 agaaagagta taaactattt cttctttatt atttttattt tcttgaaccc acctttcaaa
     1741 atcttcaaga caattttcct cttgtccaaa aactttatgt ggaaaactta taaaacacaa
     1801 aactattata ctcataaaaa taaatatctc atactttttc atcttacact cctagaaatt
     1861 tttagctttg aaacaatcct gtcgaaacat atacttcacc cactataatt tttccacaat
     1921 tatcaattat tgtcaattta tataagtatc tttattaatt gttataaata aaaagctact
     1981 tactaaagca aacagacgtt tcactttaat aagtagctat atttttataa caaaatagtt
     2041 ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat tttaactctt
     2101 atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa gtcatgtagt
     2161 gatttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa tacttccata
     2221 agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga aacctctgta
     2281 gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt gataccatat
     2341 tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa ggaaggagct
     2401 actactccat atccttttat cttggcatct attaaagcac tttctatttt atcatactca
     2461 tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc ttcaatttta
     2521 aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg taaatcaata
     2581 tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa aaactctaac
     2641 tcagaaaatc cttgtataat accttctata tcccttattt tacctatatc tataatactt
     2701 tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag accttcaacc
     2761 cattttggta agttgattct tatttctgta agtggaaaat catataatac agcttccata
     2821 acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac tggaacttca
     2881 tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg acttaacgta
     2941 tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac tctctcttct
     3001 ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa gacaactatc
     3061 cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc cttttcaaat
     3121 gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc ttcctcatgc
     3181 cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt taaagatact
     3241 gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc tactgtcatt
     3301 atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt tttaaattca
     3361 ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt tgattttcct
     3421 gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt ggatatatct
     3481 tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta aattatattt
     3541 agagttaagg gttaatattc ttaaaataga aaaataaaga ctagcaatat taatttcccc
     3601 tatatacaaa aactagtaaa ctacaatgct aattgaaatt attaaaatcc attacattaa
     3661 aagacataat tatagtcatg ataattacaa attatatata aaataattta ttatatcgta
     3721 tatacataga ctatgctaag taaaattaaa atagccctct ttatattaaa gagggctaaa
     3781 acaaatctat tattttttta ttttattata caatagttta tatatcatca cctaaaacta
     3841 cttcttccat ttcatgcttt ttacttctca tcattagttc tattccaact tctttaggat
     3901 ttgaaccttt ctttattaca gaatatatag cattagttat aggcatatct atatctaatt
     3961 tttcagcaac atcatgtgct acttcagtag cagttatgcc ttctacaacc atctttactt
     4021 cttttaaagt atcttctaaa ctcatccctt tacctatgag tataccagct cttctatttc
     4081 tactatgcat acttgtacaa gtaactatta aatctccaat acctgaaaga cctgcaaagg
     4141 tagacatatt agctcccata gcaattccaa gtctgctcat ttcactaata cctcttgtca
     4201 taagggcagc ttttgcatta tcaccatacc ctaatccgtc acatatccca gctccaaatg
     4261 ctataatatt ttttaatgct cctccaagtt ctactccaac tatgtctgga ttcgtataaa
     4321 ctctaagttt agggctcata aataaatctt gtatcatctg tgctattttt aaatctttag
     4381 aagcaactac tacggtagta ggaatatccc ttgctacttc ttcagcatga gatggcccag
     4441 atagtattac atatggattt tgtggtaatt cttcttcaca gacttgagat aatctaagcc
     4501 cagtcccttt ttcaagacct ttagctacat taacaataac ttgtccttct tttataaatg
     4561 gttttatttt ttggcaagta cttcttacag cctgtgatgg aactgctaaa actattacct
     4621 tacaatcttt tatagttttt tctatatcag tacttatagt tatattattt ggaaataata
     4681 ctcctggtaa atagtctgta ttttctttag ttctgtttat ttttttagct tgttcctctt
     4741 ttcgagtcca catgctcaca tcattacctt tttttgcaag ccctaaagct aatgcacttc
     4801 cccagcttcc agtacctaat acacatactt tctccatgtt taacaccacc tgagtataat
     4861 atatttacta ttctttcttt tgaccaattt ttctctctgt cccatttaat aatcgcttta
     4921 tatttgctct gtgattatat attactgatg cagttaagaa cagagctact attaaaccag
     4981 ctttattttt taccataatc ataaatattg gtgaacatcc tattcctaca acagaaccca
     5041 atgaaacata ctttgttata gctaccacca atataaatac tgctagacac attagtgtta
     5101 ttactggatt tacagctagc attgccccta gtgaagttgc aacacctttg cctcctctaa
     5161 atcctaaaac cgctggccaa ttatgacctg ctacaacaca tataactgcc agatatccag
     5221 ctaaaagagt atctattcca accaatcttg ctgcaaattc tgatattaaa acagccatta
     5281 cacctttaag aacatcacct aaaaatgtca ttgctcctgc tctttttcca agtgtcctaa
     5341 gtacatttgt cgaacctgca ttaccagaac cttgagtcct tatatcaacc ccagcaattc
     5401 gctttgcaac tatgtatgag gtagatatat ttcctaaaag atatgcaact acagcaataa
     5461 ttatataact aaatatctcc actgaatttc ccccctttag ttatatttta agatatttat
     5521 tttttatttt tttgtctata ctcaaacttt atggaagttc cttcaaatcc aaaattttct
     5581 cttatcttat tctcaaggta tctttgatat gagaagtgag ttaaatcctt atcatttata
     5641 aacaatgtaa ttttaggagg tctaatgtca gtttgagtcc catagtaaat ttttaatctt
     5701 ctacctttgt cagatggtgg ttgatttaac attacagctt ctccaattac ttcattcagt
     5761 gctgaagttg atattctctt agaatgttca tttgacacat attcaactgt atctagtatc
     5821 ttgttcattc tttgattagt ttttgcagaa acaaatacga ttggagcata catcataaat
     5881 ggaaattttt cttttatatc ttttgtgtaa ttgctcatag tcttattatc tttctctatt
     5941 aaatcccatt tattgattac aaatatgcat ccttttcctt catcatgtgc tatacctgca
     6001 acctttgtat cttgttctgt tacaccttct aaggcatcta ttactatcaa tactacatct
     6061 gctctatcta cagcactcat agctcttatt acactgtatt tttctattgt ctcatatatt
     6121 ttactttttc ttctaagccc tgctgtatct attagaagaa atttctgtcc atttttttca
     6181 aaataagtat caattgcgtc tctagtagtt cctgcaattg gacttacaat tactctttct
     6241 tctccaagta tcttattgag tatagaactt tttcctgcat ttggtttacc tgttatagct
     6301 actcttatta tatcttcttc atactctgta tctaatcctg ctggaaaatt ttcaactatc
     6361 tcatctaata aatctccaaa tcccatacta ttggctcctg aaactgcaaa aggcgttcca
     6421 aatcccaact cataaaaatc gtatatgtta tcaaattgac tttgactatc aatcttatta
     6481 accactaaga ttactggttt ttttgtcttt ctaagcaatt gtgcaatttc tttatcagca
     6541 gctgttattc cagctttacc atcaactata aaaagtatta catgtgacat gtccattgca
     6601 agcatggctt gatttctcat ttgagataat attatatctt cgctatcagg ttcaattcct
     6661 cctgtatcca ctaatgtaaa atatttatct aaccattcta cttcagcaaa tattctatct
     6721 cttgtaactc caggtgtatt ttcaactatt gatatccttt ttcctgcaaa tttattaaat
     6781 attgtagact taccaacgtt tggtctccca actacagcta caactggtct acttatactc
     6841 attttatttc tccttccatc ttttgttttc tattttaaaa ttttatccac aaaatctttt
     6901 ccttcattca aacaagggat aatttttatt ctcataagtt cttctaattg atttaattca
     6961 atattatcta agaaaatttc ttcatctgct tttagcatac ttcttgttat ataaagagct
     7021 tctcctaatt ctttatcttc aagttgttct tttaaatcct tagctgttaa aagacctgat
     7081 acagttattg tctctccaaa aaacttgttt ttgatttcat atacatttac ttgaagattt
     7141 ttaaacttgt ccatcatagc atctgccata ctctgtataa attcatatgc agaatgacca
     7201 gtagctattg atactttctt ctctcttttt agaatcttct caggcaatat atttagatac
     7261 tcttcaatct ctgtttttaa cttacgtatc atacctacac catcttcaaa ctgtaaaaaa
     7321 ccttcatatt cttcatatcc aggcaattca ctattagata gtatataaaa ttcatctgat
     7381 aaaaatgcaa atcgagtacc taatttttca agatatttct gttgtatttg atgtatttgc
     7441 tctatagttt tacttgcgct cttatcatta aatatattta attctactag attttctcta
     7501 tgctttgtaa tcccaactgg aactatcgca acactgttta catacggata aagttgagct
     7561 agttctttta ttgttctatc caattccttt ccatcattta tacctggaca taaaacaatt
     7621 tgacagttca tttctatatg agcatctgcc aatctcttca ttataccata taattttcct
     7681 gcaaatttat tgcttatcat tttttgtctt aactctggat tagttgtatg cacagatata
     7741 tttataggac ttattctata ttttataata ttatttatat cttcttcact catatttgta
     7801 agtgtgacaa aatttccttg taaaaaagat aatctcgaat catcatcttt aaaataaaga
     7861 gtttctctca taccttctgg caattggtct ataaaacaaa acatacactt atttctacag
     7921 cttttagctt ggtcaattat cggatttgta aattcaacac ccaaatcttc atcatagtct
     7981 ttttctatct cgtatatgta gacttcgcca tctttttttt gtatttctac atctaaatat
     8041 tcatcactta gtaagaatct atactctatt atatcatgta ttggctgttc attcacagat
     8101 ataagtaaat ctcctacctc tatacccatt tcttctgcaa tactatcttt ataaacctta
     8161 cttataatat tatttacttt ttttacattt acttccaata ttttcaccac tcttttgtat
     8221 tcatcaatgc ttattataat gtattttaat atgcaattta tttatttatt atattttatg
     8281 atagcatttt taagcatttc atgtcataat aacacatttt aattctaata gtcaatataa
     8341 gtaaatagct ataaaataga ttagaacaaa taatattcaa atttaagttt cttaaaaatt
     8401 taaatattat ttgttcttaa aaaaatttta taaatttaat gttttactat gattaacgtc
     8461 ccatttgtca tatcatgaac cattccacct acacactttg acatcaacaa tgccttattt
     8521 tgtaagtctt tctcatcatt tgcataaaat attggacaac caccaccatt tttcatattc
     8581 ttatcaagtg taactatagc tagggtatat tcatttaaac ctatatccat tcccatctta
     8641 tgcacttcct ttctttccta ttttataatt ttttagtgat aagttttttc ctttagagct
     8701 tgaaagtatt gggcatgact ttacagcttc cattactttt tccacattct tatctactgg
     8761 taaataagca atcattatac tttcatcctc tttatttctt ctaggtaatg gtacgaatga
     8821 aggttctcca taatctccat ataaacctaa ccttgaatat atattgtata ctattgcttg
     8881 tctttgaccg gagtcaaata atattcctgc attagcataa tccttatttt taggaattat
     8941 ttgtattcct acacctctat taatatatac ttctctatcg ctttttagac ctatatttgt
     9001 tatacctttt aaatctccca cctttagtat agattcgtcc acaaaactaa tttcagcagg
     9061 cattacatca gctatgtctc caatactttt tctagtaaga agttttttta atataaaggc
     9121 taacactaat ccacaaattg aacttattac caaactcatc ccttcactta ttttcgtttc
     9181 tgcaagtatt acataataca atcctattgt taaaaaagat gtaactatac acatataatt
     9241 tcttacttca taggttctag ctatctcttc tatgaatgca gctcctctct ttacaagttg
     9301 aacttcctct aaattttgca atgtatttct tctatttgct ctcacttgtc taaattgttc
     9361 tgctgctaga gataaaaatg ttatggatgt ataagaacga tttataagag ctggaaccaa
     9421 taatgctcct aatgaagaag cgacgaatgc taatataagt tgagatagta aaacatttgg
     9481 ttgcgttgga tactgttttt ggtctaaatt caacattata attctagatg ttataccaat
     9541 aataacagca acaaaaaata ctctatccat taaatcactc ctaaaatatt taatttgaaa
     9601 ttttacatgt aaatctacaa agtctttcta gaaaaatcac tctgctttcc ttttgatgtt
     9661 tctatgatag gtgtactttt cactacatct atcatgacat ctatatcttt gagtattggc
     9721 atatatggta taaccacagt acttttttct aaattggtct tagatatagc aacaatatca
     9781 acctcatcta catctctatc tatccccata tgaataaata gattatgtat tatagcttgt
     9841 ctttgcccta tatcatttac tattccaaat gcacctaaat ctcttggtat aacttctata
     9901 gccagacatt catttctata cttttctctt gtactttcta gacctatgtt agttattatt
     9961 acaccattta ccattaaaat aggtccttca aaacttattt ttgcaggaac tatatctgct
    10021 atatctgcta tactgttcct tcttaaaaat cttctaaaaa tcaaaccaac gatagctcct
    10081 gacacaatag ctaagattgt gcaataaaaa aagctaaggc catactttct tgcaaaaacg
    10141 atataaacta tcgaagatac cagtgcagaa aaaagactta tataacttct agattcatac
    10201 gtagatgcta tttcttcaac gtaagcgtct ccttttggta ctaattcttc attatcgata
    10261 ttctttagtg ttattctttc ttgttctgca agaccttgaa actgttgtat tgctactgca
    10321 aaaaatgtca aggctgcaaa ctccttatct ataagtgcag gtaatgctat agcacctaac
    10381 gaagctgaaa gcccagatat aattatctgt tctaaataat cttgtggtct tgatgggtat
    10441 tgtttgtctg taactcttaa aactaatcct ctacataata ttcctacaac taatgcaact
    10501 ataaaagagt gtctaaataa atttgcattc aataaacttt cttccatttt ctcagctcct
    10561 tctcttatat agtttgtgca aaaaacaaaa aaaattccct tctatttaag gaaatttttt
    10621 tgtaatattg taactataaa ttcgctacca atgccaagtt cactttttac ttttatacat
    10681 ccatttagtg atttgactat atgctttgtt atagccaatc ctaaacccgt tcctcctaca
    10741 tccctacttc ttgctttatc taccctataa aatcgttcaa atatgcgttc tacatcttct
    10801 tttggtatcc ctacaccatt gtctataact ttaattacga ttttttcttc atcttttaac
    10861 actacaactt gtacaatccc attatcttgg gtatacttta tagcattatc aactaggttt
    10921 aagaacactt gctttatata gtctttattg gatagtatac ttattgattt atcatcacat
    10981 ttatataaaa cttctatatt tttttcacta gctgaatgtt gagttatttc atatacctct
    11041 ttaaatacat catatactac aacattttcc ataagaagag tatctttatt ttctacaaat
    11101 gataacaaca atatatcatc aatcaacctt tttaatctat ctgattcgct ttctattatt
    11161 tctaagaatc tatttctagt attcacatct atattttcat ttgactttag tgtttcaaca
    11221 aacccactta tagatgtaag tggtgtttta agttcatgac ttacatttgc aacaaaatca
    11281 cttctcatat tttcaagttt aactttttta gtaatatctt ctatatttac tatcgaacct
    11341 attattacgt tgttagtatt ctgaagataa actgggtcta cttttatctt atatacgata
    11401 tcatctttag tagtaatatt agcagtttca ttatctttag aaccaataaa ttgaagtatt
    11461 tgttttaata tttcttcttc ttttattaca acattaatat tatgaccttc aactaattga
    11521 tttggttcac tctttatcat ttcttttgct tcatcattta tcagaagtat atttccattt
    11581 acatctatag ctaaaattcc atgagatata ctctttaata tagaccttaa ctgtaaatgt
    11641 ttatactgta cttcttctaa ggttttatcc atattcttta tcatatgatt aaaatttcta
    11701 gctaattcgc caagttctcc tttggctgat atgttaagcc tagcatgata ttcattatta
    11761 cttacttttt ttgagacata tataaactct tttaaatatt tccttaatgt tattgtgtat
    11821 cttacagcta atataacaat ggctatggac aacataacaa taaaaaaata tatactattt
    11881 aaatcttcca caagataaca ctcctaaaat ataacctttt taatctattt tgtatcctac
    11941 acctcttata gtctctatat atttttccga tttatcttca tcttctattt tttttcttaa
    12001 atatcttata tgaacatcta ctgttctagt ttctccatag tattcatacc cccatatttt
    12061 atccaaaaga taatttcttg aaagtacttt tcctttattt tgaattaata attttaacaa
    12121 ttcaaattcc ttaagagtta actctatttt ttcagagcct ttggtaactt catgttttga
    12181 taaatctagt tttaaattac ctgtagtaag tatattgttt tcttcaccta agctagtcaa
    12241 attatatctc cttaaaacag aactaatcct agctaacaac tcttttatac taaatggctt
    12301 agttatatag tcatcagcac ctatttcaag cccttcaacc ttgtcatttt ccatattttt
    12361 agcagtaagc attataacag gaatattttt taagtcctta tcacttctta tttttctcaa
    12421 aacttctatt ccacttatat ttggtaacat ccaatctaaa agtattaaat ctggtttaat
    12481 ttccttagcc tttataaatc cgtcaaatcc atcatatgaa taactaactt cgtaatttga
    12541 cacctccaaa ttgaatttta atagctcaac tatatgcatt tcatcgtcta taacaagcac
    12601 tttagtattc attaaaaagc tcctctctat tttatctgta gtaccgttcc tattcgttct
    12661 gatgccttat tatgttttct ataagagtgg aatttatctg ctctacaact agtgcataaa
    12721 tttaaattta ttatattttc ttttttaact ccactacatc taagcatata ctcattaatt
    12781 ttccataaat ctaaatagta acttccctct tttattatat agaacttttc atcattattt
    12841 gtaagaattg tgttaaattt ttctactaaa tcttcagaca cttcataaca acatggtcca
    12901 attgatggcc caattataca tactaaatct tcaggaactg ttttatagca ttcagacatt
    12961 aattttattg ttttatgacc tatattacta aaagtccctc tccaacctgc atgagccagt
    13021 cctatggctt tatttttagc atctataatt gatataggta cacagtctgc tgtaaacagt
    13081 aaaagtggta ctttttccaa gtttgttata agtgcatctc catctcttct tttccctata
    13141 gtatctttat tgactatatt aactatatct gaatgtattt gagaattact agttagattt
    13201 tcaaatgtca aatcacattc tttacatact tttaacatat cttctctatt ttttgcatct
    13261 atattcttac atgttataac tagatttaca gaatttagtt tttcatcaag ttcttcacaa
    13321 ttaccactta aattaacata ctctttaata tcttgtacat tttttatatt tatataatct
    13381 ttcatcattt gtctcctttt tctatcagta tacttttaag ttcatttaca aaagtattta
    13441 tatctttaaa ttgtctatac acagaagcaa atcttacata ggcaacctca tctaactctt
    13501 tcaatttatc cattaccatc tcaccgattt ttttggtttc aatttcttct ataaaacatt
    13561 tatttatttc attttcaata tgtacaacaa tactttcaat ctcgtcaata gaaacaggtc
    13621 ttttttcaca tgcttttagc aatccatatt ttatcttttc tctatcaaaa tattctctac
    13681 tattatcctt tttaatgacc attaaaggag ttgtttctat tttctcatac gtagtaaacc
    13741 tctttttaca tgattcacac tcacgtcttc ttctgataga ttttaaatct gtatgtctag
    13801 aatctattac ctttgattct ttataattac aataaggaca ttgcatctat attcctcctt
    13861 tgtttaacta tatctcaaac tcatctaagc ttaattctga acctatattt actattattg
    13921 tatcacaacc tattttaagt atatcattcc aaaatataac ttcttcatcc cctcctcttg
    13981 aaaaaaaact tctacttcca tctccaaaaa tggtaaatga gactacctta ccttcgtgaa
    14041 tatccatgtc tatgtctatt ataaacccca tcctttttcc attttttaca tttataattt
    14101 ctttttccat tatatcagaa actctaatca tattgtactc accctttctt taaaaataaa
    14161 tatgtatcga agtaaaaaaa aatgacctat ataggtcatt taaattaaaa taatgtttac
    14221 agtccatgga ttacttatac atattttctc atattcttta aagcattttt ttcaattctt
    14281 gatacttgtg cttgagatat accgatttca tcagcaactt ctatctgagt tcttcctttg
    14341 taaaacctta aatctaatac caacttttct ctactattta atttcttaat agcttcttta
    14401 agtgatattt cttgcaacca attttcatca gtatcttttt tatcttgaac ctgatccata
    14461 acgaatattg catctccatt gtcttgatat actgggtcaa acagagatat tgggtcttgt
    14521 atggcatcaa gtgccattac aactgattct acttctaatt ctaattcctt tgctatttct
    14581 gatactgtag gttctttaga atttgttcta attaatcttt ctctaacttg tagtgcctta
    14641 tatgctatat cttttaatga cctacttact ctaattgggt tattgtctct tagatacctt
    14701 ctaatttctc ctataatcat aggaacagca taagttgaaa accttacatt ttggcttaaa
    14761 tcaaagttat ctatagcttt tataagacca atacaaccta tttgaaataa atcgtctata
    14821 ttttctcctc tgttgttaaa cttttttatt acactcaaga ctaatcttaa attccctctt
    14881 acaaactgtt gtctggcttc ttcatcacca ttttttattt gtaataaaag ttccttcatt
    14941 tgcttatttt taagaactgg aagttctgat gtattaacac cacatatttc aaccttatta
    15001 acttgcatat attttcagtc ctctctcaaa agtattcatc tattgaaatt atttacattt
    15061 ccaatttttt tatacttttt aagaagaatt aatttagcat tatacaaatt ttttcatttc
    15121 tttttgcaac cttgagatta ttttcttttc aagtctagat atgtaggact gtgatattcc
    15181 aagcattcca gcaacttctt tttgtgtttt ttcaatacct ttatcaataa gaccaaatct
    15241 taattccata atttgctttt ctctgtcact taatctgtca agtgccataa ccaacaaatc
    15301 tctatctatt tcttcttcaa ttattttgta tatttcatca ttttctgtac ccaatacatc
    15361 agataataaa agttcatttc catctaaatc tatatttaaa ggttcatcaa atgatacttc
    15421 tgttttcttt ttattatttt ttcttaagta cattaatatt tcattttcta tacatctcga
    15481 agcataagtt gccaatttta tatttttatt cagtttaaaa gtattaactg cttttatcaa
    15541 acctatagtt cctattgaaa tcaaatcttc tacatctata cctgtatttt caaattttct
    15601 cgatatgtag actactaatc taagatttct ttctattaaa attgacttta cactttcatc
    15661 tgtttctagc ttttgtaaaa gctccatttc ttcttctggt ttcaatggag gaggaagtat
    15721 atttgcccct cccatatagt atattccctt tggctttatt atttttaatt taatacctaa
    15781 catagtaata aagcttatta ttctttcttt caatcgtaac attccttgtc ccccttaaat
    15841 tttcacctaa aatccaggca atatatttgg attcattatt gctccatatt ctctatcatt
    15901 aaaatttgaa ataccaagta ttatatttcc tatcttttgc tcatctactt ctatatagtc
    15961 agcttttaag cctattatta tgcttgtctt attacttcca gcatgtttat aaggtattat
    16021 tctaactcta cctgaagttt tatcatctaa acttcctata attttttgtg ctttcttcat
    16081 atctatatct tcataattaa tatttaatag tttttctgga ataaatcctt ctaacacact
    16141 agacttaact attattacat cactttgact tattgggtct tttaacaaat taccactatc
    16201 tactaaagcc ttacagcaaa aacttttttc tagtaagttt atatttatag tctttgtaag
    16261 ctccttaatg tacttaagca ttttcaaatc tttataaatg tattttaata attcacaact
    16321 cacataagta catgcaatta agaaagatat ttttaagtga cttattcctg taaaataaat
    16381 tataaaataa gtgcttccag aaataaaaat gtttactaga taaaaaacta acgctacatg
    16441 aacataatct tttttgctct tacttctaaa tgaaattaaa gttattatac tcatgattat
    16501 tattttaaat ggtacagtaa atagtatatt cattgatgga tacaaataag caacagaata
    16561 ggcacctcct aaactagccc ccaataactt atttttttta ggattatatc ttttagtaag
    16621 aatagaagta caacttataa taatataatt tattactagg ttttctataa catagtactc
    16681 aatatacacc atgtatcccc ccttaaaacc tttaaacata ttataagata gtatgtacaa
    16741 aattttatgt cacttttgat ggtcgaatcc aaaacaaatt ctatttaaaa agaacaatga
    16801 tatatgtctt atactttatg tcgtcaaaag atattttttt aatgccaaac aaatagactt
    16861 ttttgtagaa tccaatatat ctaggtagaa gttattcgct ctacttttgg tatatttaca
    16921 aaaaaagact acataattta ataatatatc aagtaaatct tttacttata taaaatcaaa
    16981 ttatatagtc tgtaaattca aatataaatt attgtgctag taatccattt gcatattttg
    17041 cagattcatt tagtgcttgt tctggagtta tgtctccagc tatcatcatt tgaagttgtt
    17101 tccataaata ttcatatatc tctactccat gaggtccaac tgtcaatggt ctatacatat
    17161 gtttatctct tgtcaccatt tcttcaaatc ttgggtctcc ttggtatttt tcacctttag
    17221 caactggagc tctaggtatt tctttgtgga aagcttccat tgattctttg cttagcatat
    17281 actcaagaag tttaaatcca agttctttat ttttacatgc tgacattaat gttaaatcat
    17341 ctactgaagc aaaacttcca actgtcttat cttctaatga tgttacatat ccccagttta
    17401 aatccgggaa tgatttatca aaagtacttg tagcagcaga ggatagcatt atagtaaatg
    17461 cagcttttcc tggtccaaat acactttcaa tcatttcatt actatctttt gataaagcat
    17521 cttctggaat atactttttc atatcattta taaagtttgc agctttcaag ccttctgcat
    17581 tattaaattt aactgatttt aaatcatcat tatatatatc tccaccagct tgccataaat
    17641 acggatacca gttccagttt aagcttccaa atactttaga accccaacct tgtgcaagac
    17701 cccactggtc gatttttcca tctccatcag tatcttttgt tgctttttca cagattcttt
    17761 taaaatcatc ccatgttttt ggaggttttt ctcctaattt ttctagtata tctttattgt
    17821 agtacaatac gcctggatta gcagcttcta taggtaaacc ataaatacca cccatcattt
    17881 ttgcatcatc taagtataga taattatctg aagttcctga tttttctaaa taaggtgtta
    17941 aatcttctac cgcacccatt tctataaatt gaggaaacat ctctgcatac atgtatccta
    18001 tatctggacc ttcacctgca cttattgctg ttgcatattt ttctgcgtag ttatcccatg
    18061 gtactatctg aaattcaatt tcacagttat tttctttttc aaatttttca aatatcggct
    18121 tccatgcatc tgcatcatgg tctgttaatg gtgcagacca tacaactaat ttttctttac
    18181 cctctttgcc tttagaatct tctttttttg aacatcctgc aagaattgat actgatagaa
    18241 tccctaccat aactaaagaa agaacctttt ttagtttaat catgcttaat cccctttcag
    18301 actttaattt gtctttttcc tcaaatgttt acatttaatt cagatttaat attaacaaat
    18361 aagattactc ttgtctaatc ataaaaaact atactaactc ctataactat attttttttc
    18421 tatacctatt agatttaatt catactaaaa acttactttt taaattttat caatatttaa
    18481 aaatattttt aagtctagcc acaaactaac tatctaaatt gaacaaaata aaaagaagtc
    18541 tattgacttc tttataagct tacatattat cttcttcttc ttaggaatgt aggtatctcc
    18601 atgctctcat catcatcttc ttcaattata cttgcctttt taggaggctc tattttttcc
    18661 tcagcaatta cttcttcttc aatttctttt acagcttgtt ttactgttgt atttaatgaa
    18721 gaacgtatgc ttgatttagg tttagtatct aaatctaagg caccattttg taatccttca
    18781 aatcctgtcg cgattactgt aatcattatt tcatcaccta aatcttcttt tatagaagct
    18841 ccaaatataa catttgcttc tgggtcacag gattccataa ctaaagttga tgcctcatta
    18901 acttcaaata atcctaagtt aggaccacct gtaacattaa gaagtactcc tttagctcct
    18961 tgtattgatg tttctagaag tggagattga atagcttgtc ttgcagcatc aattgctcta
    19021 gtttcaccac tagcactacc tatacccata tgagcaagtc ccttgtcttt cattatagta
    19081 gtaacatctg caaagtccaa gtttattaac ccttcaactg ctataaggtc tgaaattgat
    19141 tgtatacctt gttttaatac atcatctgca actgcaaaag catctaacat tgatgtattt
    19201 ttttgtacta tctgtaaaag tctatcattt ggtatcgtta taagagtatc tacttttgat
    19261 ttaagttctg ctattccacc ttcagcgttc ttcattctta tcttaccttc aaatgcaaaa
    19321 ggtttggtaa ctacacctac tgtaagtatc cccatttctt ttgccaagcc tgcaaccact
    19381 ggagcagctc ctgtaccagt accaccgccc attccggctg ttacaaagac catatcagct
    19441 ccttgaagta actttactat ttcatcttta ctctcctcag cagctctttt ccctacttct
    19501 ggatttgctc ctgctccaag tcctctagtt aatttttctc ctatttgaac tttatattca
    19561 gcttttgatg tatataaagc ttgtttatct gtatttactg atataaattc aacgcctttt
    19621 agttgagctt ctaccattct attgacagca ttgtttccac cgccacctac acctattact
    19681 ttaatttgtg cacattcttc taattctacg tcaaagttta gcatcataaa cccccttctc
    19741 tcatgattca aagttatcgt atgtattaat tatatcattt tcttctttta ttttgttaac
    19801 ctttaagtaa catattccac aaaaacgtaa attttccttt ttaaatttct tacttatatt
    19861 gtcaaatatt actctttaag tataaaataa tttacgtata tattaaatgc tctttaattt
    19921 ttacgttttt tgttcataat attactaata atatatcgtc taattataga taagttatta
    19981 aacaatctca taccaaaaac taatacagca acatagtaaa gagaaactcc taacttttct
    20041 cctatataag tcaataatat agccaatata gcattaccta tgaaaccagt aataaaaatt
    20101 acattatcat agttatcttc aaaattggat tttattgctc caaaaataga atccattatt
    20161 gcaagaatag ctactgacat atatatggaa taatccatag aatatgtaaa tggaatatag
    20221 taacctacta ctacaccaaa tgctaatcct cctaaaaccc ataacataaa tcttcacctc
    20281 tttattttac tttgaaacac aactacatta caatcctttt tattaatttt ttttactttt
    20341 tctttatttt tttgtatttt tcgttttatt ttgcaaataa attcacttta tttccattat
    20401 tttcctgaac ttttatatta attccatata tatccttaag agatttagca tatgattctg
    20461 gagagtttat agctgcactc aataaatcta tatctcctat agcttttatt ataaatggtt
    20521 gtccatatgt agtatcattt actgtaatag tagctcctga acattttatc ttacttgttg
    20581 ctagtactct ttctccattt atagaaattg ctcttgctcc agcctttttt aaatcattta
    20641 aaaccctcaa aatatctata tcatgtataa tcaaatcgtt tggattctgt ccgtctttta
    20701 actctctctc actatccctc atagtaatag ttatgcctgg ccctgtaaca gttgaataac
    20761 cacttaactc tttaacttct tcaagctctt tttgcataag tgtttggata gattcatttc
    20821 cattcttttt tgcttcttta tattcattca aagttttttt gttcttttta agagtttctt
    20881 ttaaattttt tatttctttt tttgtatttt ctacctcact ttcaatactt tcagtttgac
    20941 ctaatgttat gtatactcta tttgggtcta gtgattttat ataagttgat atgaaaacgc
    21001 ctaatatgaa tgctcctaat ataactattt tctttttcat actattattt cccctctgct
    21061 ttgtttatat cactatgacc atttatttct atattgtctt ctttggtcac ttttactttg
    21121 gcattataat atgtttctat ttcccagaca ataccatact ttatattaag tgctgaagcc
    21181 aatgtatcag aatcaccaat tgcttttatt ataattggtt cttttatgac agtgtcattc
    21241 atataaagct tgtcttcttt taaatacata tatgtattag aaactattct ctggtcgttt
    21301 atagatatgg cacttgcttg tgctgaattt aatttattta tcattgataa taataaatca
    21361 taattatata gtatactatc atttttatca gagtttccta agtcttttgt agaatttatc
    21421 tcaattttta tgcctgttcc ttcaacatct gtatatccag ctaatgtttc atatttttct
    21481 atttctgact taagtatgtc ttgttctcct tcattgtttt tgcctctata agtctctata
    21541 tctttcttca cactattaac ttctgattgt agattctctt tttctttttt taatgattta
    21601 agttcttctg taagttgctc tcctctttta aaagttgtca tccctctatt ttctatatta
    21661 ataattttaa gttgtaaact aacaagaaca cccaatacaa tacttcctat gattatactt
    21721 ttatttataa atcttttttt caatatatca cttccttaca attgtctatc cttctggact
    21781 atacactgcc aactttccat agttaagatt tatagtccct ccattagtat ttttattttg
    21841 caggtcaatc agaattttac ttaccctaga tatattataa tctaagttat catcattaga
    21901 aagtaagatt tttatattac ttcttgtaag catttctata ttgctttctt tttttaagtt
    21961 tacataattt atttttctat aaattccttc ttcttttaaa taatttaaag ttttaaatac
    22021 attctcctta gttttataat ttttaaattt tatagattta ttatcatcaa tgctataatc
    22081 tacatctact atcaagtctt ttttactttc attagaacct cttagttctt ccaataaatt
    22141 tcctttttta tctatataac aataattatc ttcatctttt aatactgcaa atatttcttt
    22201 ttcttttaaa cttataatta tcttattggg aagttttctt tttatttcca cattttctat
    22261 atatggattt tttataagtt tatttttcat atctttaaaa ttgtatgcaa aaatattttc
    22321 atttagattt acatttagtt ctttcattat atttgatttg gtcaccctct tatttccaat
    22381 tacatctatt ttctttacat caaataaatc actttttaca attatacata tacaaaaaga
    22441 taacataagt ataaaaacta aaactatcat gacattattc gtatttaact ttcttctttt
    22501 tttcaaatat aatcaacccc tttggttaat aaatacacct ttagagcttg tttttcctac
    22561 aaaaaaatta taatatttaa agtcaaattt ttgcatttta ttacataaca tccaaataag
    22621 gtattaaatt ttataaat
//
LOCUS       DAEMHZ010000054        21942 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000054 DAEMHZ010000000
VERSION     DAEMHZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.054"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="KPK72_001983"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="KPK72_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG2489200.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="KPK72_001984"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="KPK72_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG2489201.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="KPK72_001985"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="KPK72_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG2489202.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="KPK72_001986"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="KPK72_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG2489203.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="KPK72_001987"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="KPK72_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG2489204.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="KPK72_001988"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="KPK72_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG2489205.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="KPK72_001989"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="KPK72_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG2489206.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="KPK72_001990"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="KPK72_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG2489207.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="KPK72_001991"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="KPK72_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG2489208.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="KPK72_001992"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="KPK72_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG2489209.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="KPK72_001993"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="KPK72_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG2489210.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="KPK72_001994"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="KPK72_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG2489211.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="KPK72_001995"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="KPK72_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG2489212.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="KPK72_001996"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="KPK72_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG2489213.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="KPK72_001997"
     CDS             6989..7174
                     /locus_tag="KPK72_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG2489214.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="KPK72_001998"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="KPK72_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG2489215.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="KPK72_001999"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="KPK72_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG2489216.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="KPK72_002000"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="KPK72_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG2489217.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="KPK72_002001"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="KPK72_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG2489218.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="KPK72_002002"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="KPK72_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG2489219.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="KPK72_002003"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="KPK72_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG2489220.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="KPK72_002004"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="KPK72_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG2489221.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="KPK72_002005"
     CDS             11117..11767
                     /locus_tag="KPK72_002005"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG2489222.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="KPK72_002006"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="KPK72_002006"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG2489223.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="KPK72_002007"
     CDS             12536..12805
                     /locus_tag="KPK72_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG2489224.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="KPK72_002008"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="KPK72_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG2489225.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="KPK72_002009"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="KPK72_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG2489226.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="KPK72_002010"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="KPK72_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG2489227.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="KPK72_002011"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="KPK72_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG2489228.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="KPK72_002012"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="KPK72_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG2489229.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="KPK72_002013"
     CDS             14901..15848
                     /locus_tag="KPK72_002013"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG2489230.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="KPK72_002014"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="KPK72_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG2489231.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="KPK72_002015"
     CDS             16330..17163
                     /locus_tag="KPK72_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2489232.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="KPK72_002016"
     CDS             17151..18017
                     /locus_tag="KPK72_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2489233.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="KPK72_002017"
     CDS             18011..18814
                     /locus_tag="KPK72_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG2489234.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="KPK72_002018"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="KPK72_002018"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2489235.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="KPK72_002019"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="KPK72_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG2489236.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="KPK72_002020"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="KPK72_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG2489237.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="KPK72_002021"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="KPK72_002021"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG2489238.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8812 a         2564 c         4499 g         6067 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tatttggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttattg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctacttcat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 tgactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactggctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag caaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacacaatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aagtttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa taggggcaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAEMHZ010000055        21457 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000055 DAEMHZ010000000
VERSION     DAEMHZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.055"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            281..1552
                     /gene="serS"
                     /locus_tag="KPK72_002022"
     CDS             281..1552
                     /gene="serS"
                     /locus_tag="KPK72_002022"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2489239.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1876..3381
                     /locus_tag="KPK72_002023"
     CDS             1876..3381
                     /locus_tag="KPK72_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2489240.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3495..4193
                     /locus_tag="KPK72_002024"
     CDS             3495..4193
                     /locus_tag="KPK72_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2489241.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4478..5566
                     /locus_tag="KPK72_002025"
     CDS             4478..5566
                     /locus_tag="KPK72_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489242.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5805..6995
                     /locus_tag="KPK72_002026"
     CDS             5805..6995
                     /locus_tag="KPK72_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2489243.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7086..8588
                     /locus_tag="KPK72_002027"
     CDS             7086..8588
                     /locus_tag="KPK72_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2489244.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAIISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKCALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFMASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8660..9916
                     /locus_tag="KPK72_002028"
     CDS             8660..9916
                     /locus_tag="KPK72_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2489245.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYV
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10088..12862
                     /locus_tag="KPK72_002029"
     CDS             10088..12862
                     /locus_tag="KPK72_002029"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2489246.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGKYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(12927..13112)
                     /locus_tag="KPK72_002030"
     CDS             complement(12927..13112)
                     /locus_tag="KPK72_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2489247.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13487..14665
                     /locus_tag="KPK72_002031"
     CDS             13487..14665
                     /locus_tag="KPK72_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2489248.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINLLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14792..14877
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14995..17889
                     /locus_tag="KPK72_002032"
     CDS             14995..17889
                     /locus_tag="KPK72_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2489249.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSSISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGGWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKKVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKKPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPMVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPVPPSEEMIETPVKQIIPIPEVVKPSVSE
                     EKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN"
     regulatory      18075..18160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18260..18922
                     /gene="zmp1"
                     /locus_tag="KPK72_002033"
     CDS             18260..18922
                     /gene="zmp1"
                     /locus_tag="KPK72_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG2489250.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19044..20006)
                     /locus_tag="KPK72_002034"
     CDS             complement(19044..20006)
                     /locus_tag="KPK72_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2489251.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20176..21366)
                     /locus_tag="KPK72_002035"
     CDS             complement(20176..21366)
                     /locus_tag="KPK72_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2489252.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8482 a         2480 c         3676 g         6819 t
ORIGIN      
        1 atacaagatt gataaagaaa aagtcaaaat agataattga ttagcaaaat agatataatt
       61 ataatgaagt taaatctagc aatattctcc taaaattaaa tttcatttat ttaattaaat
      121 ataatttgac aatgggcaaa aaagtgagta aaatataaat aaaataaaaa taacaggttc
      181 ggataaagaa aaaggtttct taacctcaag cataaagtat attatctaag ttatttgaga
      241 ttaagagata aatccaatct taggtactga ggaggaaaaa atgcttgata taaaaagaat
      301 aagagaaaat ttagattgta taaaaaaagc aatggaaaga aggggcgaaa aagactttaa
      361 ccttgatgaa gtagttaagt tagatgatga aagaagaaag attcttcaag aagttgaagt
      421 tatgaaaaat gaattaaata cagcatctaa aaatatacca aatcttataa aagaaggtaa
      481 agatgtagaa aatgaaaaga ttaaactaaa agagttatct gataaaatta aggtaataga
      541 ccaaaatctt aaagaagtag aagataagat ggaataccta ttaatgagaa taccaaatgt
      601 accacatcca gaagtaccac aaggtgaaac tgatgaagat aatgttgaag taagaacttg
      661 gggaaagact actacctttg attttgaatc taaggctcat tgggaaattg gaacagaatt
      721 aggtatactt gattttgaaa cagctagtaa aataactggt tcaagattta ctctttacaa
      781 aggattaggt gctagattag agagagcatt attaaatttt tacttagaca cgaatacaaa
      841 agtaaatgga tatacagaag ttattccacc atttatggca aatagaaata gttttttagg
      901 tacaggacag ttacctaagt ttgaagaaga tatgtttaaa atagaaggtc tggactattt
      961 tatgattcca acatcagaag ttcctttaac taatatacat gctaatgaaa tacttaaatt
     1021 tgaacaatta ccaataaatt atacagctta tactccatgt tttaggtcag aagctggttc
     1081 tgctggaaga gatacaagag gtttagtaag acagcatcag tttaacaaag tagaaatggt
     1141 taaaatagtt gcaccagaag aatcttacaa tgagttagaa aagcttacta acaatgcgga
     1201 aactatgcta caattattga atctgcctta tagagtagta aagatatgta caggtgattt
     1261 aggatttacg gcttctttta aatatgatgt agaagtttgg atgccaagtt acaataggta
     1321 tgttgagatt tcatcttgtt caaactgcga agatttccaa gctagaagag caggaataag
     1381 atttaaaaga gataaagatt ctaaagctga atatgtacac acattaaatg gctctgggtt
     1441 agctattgga agaagtgttg cagctatatt agaaaattat caacaagaag atggttctgt
     1501 ggttgttcct gaagttttaa gaccatatat gggagtttct gttattaaat aagagttaaa
     1561 ttatgtattt ttaagcaata caataatata gaggtaataa aaaataatta atataaaagt
     1621 gatacaataa tatagaagta ataaaaaaat agttaatata aaagtgatac aataatatag
     1681 aagtaataaa aaaaaagtta atataaaagt gatacaataa taatagaagt aataaaaaaa
     1741 aataattaat ataaaaatgt gtacaaacta atgaatattg atatagtaag ctatcatatg
     1801 aaagagtatg tacacatttt ttttactaat ttttcacaat atgaacataa ataatttaca
     1861 ggaaaggaat cttttatgaa aattcaaaat actgagtggg gatatattga atggaaacat
     1921 acttatgatg aaaataatcc aaaacaagct atgaatatat acattgcagt aactatgcct
     1981 ggtaagaaac attttaatca tgttcattat ggtcaagagc agatgatata tattttagag
     2041 ggagagggtt tgtatattat aaatggagtt tggaagccat tttatcaagg tatgattttt
     2101 tatatagaat ctggtagcac acatgaaact ataaatacag gtgatagaga aataaaagaa
     2161 cttattgttt caaataatgt agatgatgta ggagagagtg aagttataga tattaatcct
     2221 aataattatt taaagaagac cttgataaat tatagtgaaa gtacattaaa tttgtatgca
     2281 gcagtagaat caattcgtgg tcaatttata gacccgttta aaattccatt aattatatat
     2341 gatgattcat ggaatatagt tttaaaaaat ccttattttc cacttttttg ttttgaaaaa
     2401 tgtaatccta tgaaatttcc tcaaaattgt gattgtatga atcaaaaaag tagcaatcaa
     2461 tttgtatgtg aatatgggat aactatatat aatataccaa tactgtacaa aagcaatagc
     2521 ataggtgtta taagaggagg gtatgtacta ttatctgatt tgaatttaga tacagagcat
     2581 aataatttat atgatattcc agagggagct gctagaagta taaaacgcct cttgaaacaa
     2641 atatctaaaa atataataaa tttttgctct tttaatgata taagaaagga tttacaagaa
     2701 aaagagaaga ctatagcaag aacatatcat tatggagagc aacttgaaat gaatttaaaa
     2761 gtagctcaag atatggttac taatttaaga ataaatcatc actttttatt taatacatta
     2821 aattcaatgg caagcatagc gctagatgat ggtagttatg atttgtatag tgcaataata
     2881 gatttatcaa gaatgtttag atatacaatg aggtctgatt taagatttgt agaattagaa
     2941 tctgaaatat tatacattaa aaattatttg aatttacaga aattacgtta tggagatgca
     3001 cttaaagtaa aatatcttat tccagagaag ttatataacc tttctgtgcc ttttaacttt
     3061 attcaaccta ttgtagagaa tgcatttaca catggattta gagatattga cacagaaaag
     3121 cgaattgaaa taattgcaag attagattct cagtatgcta ttatagagat tcataataat
     3181 ggaactatct tagatggaaa taatatagac aaaataaagg caggtattcg tagtaataat
     3241 ggtcatggtt tgtctttaat atatacaaaa ttcacatctg cttatggaaa taattttgat
     3301 atggatataa agtcgagtga taatgaagga acttacataa tgattaaaat tcctattgaa
     3361 aattataagg agtatgtata gaacttgtga aaaattaata agttttataa atgttaagaa
     3421 atatattaga tgtttttatg ttgttttata tatcttactt ttttatatta ttaagctaaa
     3481 agggaggtat tctcatgaga agagcactaa taatagatga tgaagaagct gttctaaaaa
     3541 taataacaca ttttatagaa atgaaagata tgccaataaa aattgttggt aaagcaacat
     3601 ctggtgatga agcagtagat aaaatcattg gtttagagcc agacatagtt tttatagata
     3661 ttcaaatgcc aatatacaac gggttagaag taatagaaaa aacatctcat ttatctaaaa
     3721 aaataaactt tgtagtaata actgcattta actattttga atatgctcaa aaagctttga
     3781 gattaaatgt aaaagatata ctccttaaac cattggatat gaaagaattt actaagtctg
     3841 tagaaaaaat tattggatat caatatacca ataatgattt attaaatgag atactggaat
     3901 atataaatct aaactatagc aatgacttaa agttaaatga ttgtgctaga ttatttttaa
     3961 ccaatccatc taatatatct aggattttta aaagtaattt aaatacaact tttatatcat
     4021 atctaaatca tataagaata gaaaaatcta ttgaattact tgaaaatact acaaaaagta
     4081 taaatgagat tgcagaaata gttggatata atagcctaaa taacttttat aaaaacttta
     4141 agatagaaaa aggaatgact ccaaaagttt ataaactgaa cactaaaaat tgaaattgat
     4201 aaataaaaag tcaatataga taattgtttg agtgtgtctg aataagtata ctttaattat
     4261 gataaaaatt aaagcgcttt aaacatattt aataatatta aaccttataa aagctgtacc
     4321 tatttatata aaaaaatata tataaataaa aagaaaaaga tatacaaaat aaaaaaagtg
     4381 aattaaatac tgtttcaaaa agacagaatt ttcatttaat tcactgagag tttatatttc
     4441 ttaaaggaag gtaactttat aatggaggta atttaatatg tcagacaaag tgaatatgaa
     4501 aaatattatc tgtattgcag gcgcttacct agcaacagca attggttctg ggtttgctac
     4561 tggtcaagaa attctacaat tttttgcctc acaagggtat atgggtttaa ttggagcatt
     4621 aatatcaatg gtattgtttt catttcttgg tgctgaagtt ataactagag gtagagaatt
     4681 aaaacttaaa gaaccaacaa gaatttacac tttttattgt gggaagattt taggaaaatt
     4741 ttacgaatgg tttagtccaa tatttttatt tgggattctt atagtaatga tttcaggtgc
     4801 aggctcaact ttaactgaat actatggtat aaatccttac ttaggaagag tattgatggc
     4861 tgttgctact ttaattacag tgtctatggg tttagaaaaa ttagtaagca tacttggacg
     4921 tattggacca gtgatagcag tatttacaat tgctgttgga ttaataagct tgtttaagca
     4981 ttttgacggt ttatctatgg ttggagaagc tatgaaaaat attgaagtta caaaagcatc
     5041 agaaagtcca atcgtttctg gtgtaatata taatacatta aatattattg taatgattgt
     5101 ttttttaaca ggaatgggag cttctattga aagtaaaaaa gatgcttttt ggggaggttt
     5161 attaggtgga atagtgttta tgactgctgg tatagttatg tatttggcta tgatttcaga
     5221 tataggaaat ttgtatatga aagaaatacc atcactttat ttagcagata atatatctcc
     5281 gattgtaggt gtatgttttt cagttgcatt aatattaggt atctatacaa cagctgttcc
     5341 acttttatgg tctgtaacca atagaattgt tgaagatgaa catcctaaat ttaaattggt
     5401 aacaacagta atagctattt tggcttgtat aggaggtttt ttaccgtttg gtaaattagt
     5461 aaacatttta tatccatata caggttatat ggggctttta attttagtct gtatggtgta
     5521 taaagcaatt acatgtagaa aaaaatcaac acaaagtcag gaatagagcc acttattaat
     5581 agtttttatt taaagaaatt ttttatataa tccgaattta aaaagtcaaa atagttaaat
     5641 aaattgcaaa acatgataat atatttaatt aaaataatat agtaaaatgt aaatataaac
     5701 taaaattaaa tttataaaaa atatctttat aaagattatt aacttatata atttgtagtt
     5761 aaattataaa aaataaaaaa ataaaatttt ggagaggtgt tagtatgcat gaacttattt
     5821 taatctcaga aaagtataaa gaagatattt tagatttttt tacagatatt catatgcacc
     5881 ccgaattaag ttttaaagaa tttaggacta caaaagctat aaaagattta ttagtaagtt
     5941 tagacataga aatattagat ttaggaatgg aaacaggagt tgttggatta ttaaaaggga
     6001 agtatgatgg tcccacagta gcactaagag gagatattga tgcattgcca atatatgaag
     6061 aggttgatat taagtataaa tccagatatg atggtataat gcatgcatgt gggcatgata
     6121 tacacacgtc ttgccttgta ggatgtgcat atgtattgtc tcatattaga gatagtcttc
     6181 atggaaatgt aaagtttata tttcaaccag ccgaagaagt caataaaggt gcgaagatgt
     6241 tagtggaacg aggagtaatg gaaaatccaa aagttgatgc tatatttgga cttcataatc
     6301 atccagatat accttgtgga aaaataggtg ttaagttagg tgggttaatg gcagcagtag
     6361 acactattaa gatagaagta aatggatttg gaggtcacgg aggtatacca aacagaacaa
     6421 tagaccctat agtagcatca agtgcaatta taatgggtat tcaaaccata gtaagcagaa
     6481 acataagtcc attggaatca gctgttatat caataggtac tataaatgga gggactgcaa
     6541 ataatgtaat ttcagaaaag gtagatatga caggcacatg tagaagcttt agtaatgaag
     6601 tacgaaagaa aatatcagaa aatttagaaa atatagtttg tgagatagct cgtggatatc
     6661 aagcaactgc aaagctagat tatttatttg atttaccagc tgttataaat agtaaagaga
     6721 tgtacgcaat agcttgtaag tctgtgtgtg atttgtattc agaagatgct atagttgatc
     6781 caataccctc tacaggagga gaagattttt ctatattcat ggaaaaagcc ccaggatttt
     6841 tttactggtt aggggtaggc aacaaagaac aagattgtat atatcagtgg cataatccta
     6901 aatttaaagc tgataaaaat tctatattgg taggtacaaa tgtactatgt cagtctgtta
     6961 taaattatat ggataaatta aaaaataaaa tttaagatag agcattctgg agataaagtt
     7021 tatgaggggg tattagtttg aggaaaatta attcacagga aaaaaacaaa gatagtggat
     7081 tattgatgag ggcactaaat gttgtagaga gagcaggaaa taaacttcca gacccagtaa
     7141 ccatattttt actactatgt ataattgttg taatattgtc agcaattata tctaatttag
     7201 gagtagaaga aatacatcct tctacaaaag aagttgtaaa ggtagtaaat ctactggaga
     7261 aagagcagat tcaatcatat ttaggtagca tagttacaaa cttccaatca tttgctcctt
     7321 taggattagt actggttaca atgcttggag caggtgttgc agagaaatct ggatttatgg
     7381 aagtgttaat gaaaaagggt ataagtaaag ttccacaaaa acttgtaact gtagcaatcg
     7441 tatttgcagg tatgttatca catacagcag cagatgtagg atttataata ttaccaccac
     7501 ttgcagcact tgtgttttta ggaattggaa gacatcctct tgtaggtatg tttgcagctt
     7561 ttgcaggtgt tgcaggggga tttgcagcaa atgttatgct tagtactaca gatgtattgt
     7621 tagctggatt tacaatacca gcagctcaaa tgatggaccc atcttaccaa ggcaatccag
     7681 caatgaatct ttattttgct tttatatcag cgatagtttt aacttttgca ggagcatttg
     7741 taactgaaaa atttatagct cctcgtttta caaaatataa tgattcaaca aatgataagg
     7801 atatacagga attgagtgaa cttgaaaata agggtataaa atgcgcttta ttatcattgt
     7861 tagttgtagt agtagtgata gttgccttat gtataggtga caatgcattt atgaaagatc
     7921 cagagactgg ttctatacta tctagtaatg cacctttaat gaaaggtata gttcctataa
     7981 tcactattat atttttaact ccaggattag tgtatggaaa agtaagtaag aagataaaaa
     8041 gtgacaaaga tttagtatct atgatgggta gtagtatgag tgatatgggt ggatatatag
     8101 tcctagcatt tatggcatcc cagtttatta atttatttaa tcttagtaac ttaggaacaa
     8161 ttttatcaat aacaggagca aaattactag cagagtcggg aatacctagc tatggattga
     8221 taataggatt tatattgcta tctggattta tcaatttatt tgtaggaagt gcttctgcaa
     8281 aatgggcgat tttagcacct atatttgttc ccatgtttat gctactagac tttaatccag
     8341 cattaactca aatagcttat cgtattggag atgcttcaac aaatccaata agtccacttt
     8401 ttccgtattt tccagttata ttggcctttg ccagaagata tgataaagat ataggtatag
     8461 gtactgtcat atcgaatatg attccatact cagtggtatt tacattaata gaaataataa
     8521 tattgttact attcatgggt attggcatac cattaggacc tggtggagga atatcttacg
     8581 ttttatagac cgttataata aatttatcaa tgaagattaa actaataaaa tatattcttt
     8641 aattaagagg aggattatta tgttaaaaac aacaaaaatt tcagacccag agttatacaa
     8701 gattgtagct gatgagttgg ttagacaaga acacaatatt gagatgatag cttctgaaag
     8761 tacagcgcca acagaagtac ttgaattaag tggatgtgta tttacaaata aaacagaaga
     8821 aggctatcca ggggctcgtt ttcaagcagg ttctgaagaa gcagataaac ttgaaaccct
     8881 agcaataaaa cgtgcaaaag aagtatttgg agcagaacat gtaaacgtac aaccgtattc
     8941 aggttctaca gctaactatt gtgtatattc atcaatatta aaaccaaatg acactgtttt
     9001 aagtatgcgt cttgaccaag gtggacactt gacacatggt agcgcagtta attttttaca
     9061 tgacatttat aaatatgaat tttatggggt agacccaaat actggacgta tagattatga
     9121 tgctcttgaa gcaaaggcaa aagaatgtag accaaaactt ataattgcag gggcaagttc
     9181 atatccaaga ttaatagatt atgaaagaat atcaaaggtt gcaaaagaag taggagctta
     9241 ctttatggta gatatggcac atgttgcagg attagtcgca gcgaaagtaa tacctagtcc
     9301 tgtaccttat gtagattttg tttcttcatc tacgacaaag actttctgtg gaccaagaag
     9361 tggtattgta ctttgtaaag cagaacatgc aaagaaactc gataaaggtg tttttccagg
     9421 aacattaggt tctatacatc ttaatacagt tgcagcaaaa gccttttcac tattatattt
     9481 aagtacagat aaatttaaaa agataatgga gcaagttgta gtaaatgctc agactctagc
     9541 ttctgaactg atttcacatg gttttagtat tgtaagtgga ggtactgata atcatatagt
     9601 aatggttgac ttaagaagta agaatcttac aggaaaacaa tttgaaaaag ctcttgaata
     9661 tgtaggaata acagttaata aaaatgttat acctgacgac ccacaatcac catttgtaac
     9721 tagtggtgtt cgtataggtc ttacatcaat atctcaaaga ggtctgaaag aaaaagaagt
     9781 tatacagata gcagggataa tgaataaggt agcagaaaat attgataata aagaagtatt
     9841 agatgaatgt aaagcagagg cacaagaact tatatctaag tttccattat atccagaagg
     9901 gtattttgaa gattaaatat tttgatttgt atagctcaca atttataaag cctgtaacat
     9961 aaaatttaat aatatataac cactataagg tgttgattaa tcaacacctt attttttttg
    10021 aaaagtaata tacaagctat cataagcata taattcctta tagataatta acaaggggga
    10081 taaagttatg aggtattata ataaacctac caaagaagta ttaaaatact taaaaacaaa
    10141 tccagaaatt ggacttgatg ataatgaggt agaagaaagg aagcttagat atggattaaa
    10201 tgaatttact ataaaagaag gtagaacatt ttgggacgag ttgggagaaa gtttgacaga
    10261 acctatgata ttaattctca taggagcagc agtaataagt tcttttgtgg gagaattaca
    10321 tgacgcatta ggtattttag gggctatttt tattggaatt tctataggca taataactga
    10381 aggaaaatct aaaaaagcag ctcatgccct gtcaaaatta acagaaaata tagaagtaaa
    10441 ggtgcttaga aatgggaaga taattaaaat atcaaaaaat gatttagtac caggagatat
    10501 tgtatatata gaaactgggg atatgatacc agcagatgga agacttatac agtctataaa
    10561 tttgaaactt agagaagata tgctaacagg agaatctgat gatgtagcta agaatgctga
    10621 tgctgtatta gatatggaag tagtttattc taaaacagaa ataatcgaac aggatgcaat
    10681 acctgcaaag caagtcaata tggtatttgg agggactctt gtagcatatg gtaggggcat
    10741 aatggttgtc actcatactg gtgataagac agagatgggt aagatagcac agaatttatc
    10801 aaatgaagac cagcaaactc cacttcaaat taaattaggg aagttgggtg ctaaaatagc
    10861 aggaatatca ggcataatag ctactttatt gtgtatgttt atgattatac agatgcagag
    10921 aaaaggtatg ttaattttag atacaagcag tatattatct ttcttacaat cattagaacc
    10981 tgctaaaaat gcatatatgg tttgtattgc attaatagtt gcaactgttc cagaaggttt
    11041 gccaactatg ataaatatta ccttagctat aacaatgcaa aagatggcaa agataaatgc
    11101 attagtaaca aagaaagaag cctgtgaaac tataggttct gtttctgtaa tatgttccga
    11161 caaaacaggg actttaacac aaaacaaaat gatggtagag gtagcatatg ttgatggtaa
    11221 atatataagt ggaggaaagt atcaaagtaa tagctatttt gaacaaaact gtatagtaaa
    11281 ttcaactgca gatatagaaa aagaagataa ttcatttaaa tatataggaa gcgcaacaga
    11341 gtgtgcatta cttttatatc ataatgataa gaactataat gaaatgagaa aacaaacgta
    11401 tttgatatca caaataccat ttagttcaga agaaaagaag atgtcaacat taattcgtca
    11461 agaggattca gatatattac tttcaaaagg tgctccagaa gtattattaa aaaaatgttc
    11521 ttatgtacaa caaggaaaaa atattgttcc aataactcca aaagtagaaa aaagtatttt
    11581 agatgagatt aagaagttac aaattaaatc tatgagaaca ttaggatttg cttataaaaa
    11641 aatgagtaat tctaaaacag aagttgcaat gacttcagaa ggtgaattga atttaatagg
    11701 aaattcaaga agttatatga aggaagataa tttagttttt agtggatttg taggaatagt
    11761 ggacccattg agagaagggg tcaaagattc cattgataaa gcatttaatg caggagtaga
    11821 tgtaaaaatg cttacaggag ataatataaa tacagcaaca gctattggaa atgaattagg
    11881 actattaaat gatgggaaaa aggcagttga agcaacatat atagatgtat tgacagataa
    11941 agaattgaga gaagaaatca aaggaatatc tattgttgca agaagtaaac cagatacaaa
    12001 aatgagaata gtttcagcac ttcaaaaaag tggagaggtt gtagctgtaa caggtgatgg
    12061 tataaatgat gccccagcac ttagtcaagc agatgttgga attgcaatgg gtatttcagg
    12121 aacagaagta tctaaaaatg cagcagatat aatattgact gatgatagct ttagtactat
    12181 agtagaggga ataaaatggg gaagaggtat atatgagaac tttcaaagat ttatacagtt
    12241 tcagcttact gtaaatatag tagcatttat aattgctata atatctcaac ttactggtaa
    12301 ggatatgcca tttactacaa tacaattatt atgggtaaat ataataatgg atggtcctcc
    12361 agctctagca ttaggattag aacctgttag agattatgta ttgaaaagaa aaccaataaa
    12421 tagacattct ggaataatag ctaggtctat gtttgtaaat attattataa atgcaattct
    12481 tataattaca attgtattta ctcaatcagc ttttaatata ttgggagcta catcagaaga
    12541 acaaggtact gttatatttt ctttatttgc attcagtgcc ctttttaatg cattaaattg
    12601 tagagagttt ggtttaaata gtacaatacc aaatttcttt aaaaataaac tggcattgca
    12661 aataatagta gttacaggga taattcaaat aatatttaca caagtgttcc aaagtttctt
    12721 taattctgtt tctttggact ttgatatgtg gataaagata atactatttg catccactat
    12781 cctattgtca aatgaatttg taaagctaat tcttagaaca atgaaaaata gtagaactat
    12841 gaactttaat agtaaaaatt aattcttatg aaaccgtaaa gtactatata ttgtaacttt
    12901 gcggtttttg tttgtttaat gttgttttat attaccattt tcatatttaa ttttttatta
    12961 atctgaacac aatcttccat tgtaattgct tcatctgtgt caacacattc aaagtcaata
    13021 tctaattttt gtaatatttt gtatgcctct ttttgcaaag gagtatcaaa tttaataggt
    13081 gattttgtgt aaatatcact gataaaaaac atgatttatt tcattcactt ccatttaaaa
    13141 tcttatagtt atagtgtatc ataaggttat aaaatatagg taaatatagt ataaatttaa
    13201 aatatgtaca gacaaaacaa aaaaatcata ttctaagtaa tacttgggaa aaatgatatt
    13261 atcataataa ataatctttg taaaaactta tatagaatct attaaacaga cactaagcca
    13321 taaaaacttt actaaatata aaattcgtat ctaaatagat atatagaata atattttact
    13381 gaagtagagt gatttttaag aattttatat ataaattatg ttatcataaa taaagatata
    13441 gattataaaa tttgttaata aaataaaggg cagggaggta agtttcatgt tttccaaaat
    13501 cctagtcaaa acatttatca gagatagcga aaacgttcaa aatacagatg ttagaaataa
    13561 gtatggttat gtagctggtg tagtaggtat attatccaat ttacttttat ttgtgataaa
    13621 ggtattcata ggaatgctca catctagtat agctataatg gctgatgcat ttaataatct
    13681 ttctgatatg gcatcttcag caataactat gataggtttt aaattagcaa gtaagccagc
    13741 tgataaagaa cacccttttg gtcatggaag aatagagtat ttatcagcat taatagtagc
    13801 atttatggtg atgttggttg gacttcaatt tgtaaagtca tctatagaaa ggatagtaaa
    13861 tccaattcct gtaaaatttg aagtaatacc acttatactt ttaattgcat ctataatgat
    13921 taaaatctgg ttgagtcgtt ttaataaatt tatgggaaat aaaattgatt cttcagcctt
    13981 aaaagcagtt tcacttgatg ctttaggaga tgtatttact tcaagttgtg tagttatatc
    14041 atttattgta gctagattta ctaattttcc catagatgga tatgttggta tcgtagtatc
    14101 attagtgata ctttatgctg gtttctctct tgtaaaagat actataaatc ttcttttagg
    14161 ggaagcacca gatgaagaaa tggtaaattc tattatagag ctattgttat cttataaata
    14221 tataataggt acacatgatt taattattca taactatgga gttggtagat gtattgcatc
    14281 tatacatgca gaaattccat ccaacataga tataatggaa atacatgaaa ttatagatac
    14341 tgcagagaga gaaatatctg aaaaattaga tatatatcta gtaattcaca tggacccaat
    14401 atgtcttgaa gataaagaag ttatgtcagc caaaaaagaa cttgaagaaa tacttaagaa
    14461 aaatagtctt gtaaaatcta tgcatgattt tagaatagtt ggaaaaggaa gcaagaagaa
    14521 tttaatattt gatatagtag ttaatccttc cgaattttcc aaaaacatgt cagaagatga
    14581 cttaaaagaa gatataacaa aattagttaa ggagataaat cctgaatata attgtgtaat
    14641 tgttgtagat aaggatttta tttaattatt aattaatctc atcataaggt aaaaaatgta
    14701 aaaaggatag acaatagtat agaggcataa tataatagaa gtatataatt gtatttttaa
    14761 tataaataaa gcaattttaa ttgtttttat gaaatattat agagatgttg aagtatattc
    14821 tatcattggg caccttatgg atatactgag tcagtggtgc aaccggctat gaatataaat
    14881 ttatttattt tcatagcttt ttttgtataa ttttttagta aaaattactt tatatacgat
    14941 attaatggaa gattagatta taaattatag gtaacctaaa ggggtgagat ttacatgaag
    15001 aaaggaaata gaaaggcatt attaattagt ttaattatga ttttaagtat ggtggtgtct
    15061 acaatatatc caactgtatc ttatgcttca gaattaggag agaatagtca gattcaaagt
    15121 ggttcaacta attcatcaac tggagaggag aaggaaagcg acaataaaaa gccagaacag
    15181 actccagaga aagataaggc aacagataat aaaaaaccag aacagacccc agaggaagaa
    15241 aaaccaacag ataataaaaa accagaacag accccagaag aagataagtc aacagataat
    15301 aaaaagccag agcaaactcc agaaggagaa aagccaatag ataataaaaa gccagagcag
    15361 ataccagaag aagataagtc aacagataat aaaaagtcag aacaagcttt agaagatgaa
    15421 aaaccactag acaataaaaa tacagaaaaa actccagagg aagataatct attagaagat
    15481 gaaaatttat tgaaagtttt agaagaagaa cttaatgaag aaaatgaaga ctatggattt
    15541 gttgtaaaaa ttaataacaa tacaatagaa acggaatcaa tgaaaaaaat atcatttaat
    15601 ttaacttata ctccaacatc aaaaggaata caagcaggag attcaataac ttttaaggtg
    15661 ccagatgtat tcaataaagt taatttagat tatacaagtg aatgctttga taaaacagaa
    15721 agtaatggag agtatacact tacatttaga gaattaccta atggccaaag tgtaatgcaa
    15781 ggtaaaatag gtttggaagc atatgttaaa aaggttgatg aagatactaa tgcaaaaata
    15841 catattgaaa ccactggaaa aatagaaagt ggaagtggag atatagatgt agaaataaaa
    15901 cctggagata aaacggatgt tccagatgct aaaggaacac ttaaaaagct tgttgaagga
    15961 aaaaaatcaa ctactgtatt tatgccagtt aaaaataaag atataaatta cagtatacaa
    16021 gtaaatgaaa agcaggaaga gttaaaagat attattttgt atgatgagct tcctgaagga
    16081 ttgacattga taaatggttc tgtaagtgta gtaacaagtg atggaaaaga agttagtgat
    16141 tttaatattg aacagagtaa aaattctatt tcagttaatt ttggaaatat agataaatcg
    16201 tatactgtta agtataaggc tagaatcagt gacaaaaatg caaaacatgg taataaatat
    16261 aagaatgtag ctaggattga atctgatggt aagaagatac aagaagatga tgctactgta
    16321 agtatatttg atagaggaga tgattatctt cttactaaag gacattcagg agcaacaaat
    16381 attactcaag ttggtcaagt aataaactat cagatttcaa taaatgatga taaatcatca
    16441 ataagtaatg ttgttataac agataatata ccagaaggca tgagattaac tacttcagga
    16501 gaagctgggc atgattttag agttgtagaa attccaatga atgggggttg gactccttgg
    16561 agtaaagaaa aaattgccaa taatatttca tataaagtag aagaaaaaag aaatgaaagt
    16621 gggcaagtag ataaagttat aactggattt acaattaatc tcagtaaaga agaagtggaa
    16681 tcaaagtttt ttatagctta tacattaaag gtaattagta ttgaagatag ctatataaat
    16741 agagctgttc ttgatgcaaa taatagtgaa attgataaaa atgatgagat aaactttaag
    16801 aaaaactctg gattaataag cgcaaaaaaa gaagttgata aaaaagtttt aaacagtagt
    16861 gataaccaga ttgttaaata caaaatcaat atgtcaactt atggagttta tgatgcagga
    16921 caagtaaatc ttttagatga agtaaactca gttctagaaa tatctaatat aaaatattct
    16981 gataatcttg aacttaaaaa agaagctgga gatggaaaaa atacaataag attagtaaat
    17041 aaatacgaat ttaaacagat aaaagaaggg gaacctgtgc aaagttgggt tacatttgat
    17101 gcaaacttca caaatgtaaa agttggggag actatacgaa atgtggctca gattaatggt
    17161 tcaagtcctc caggagtaga gactactaag caaggttatg cttttgaagc taaaaaagtg
    17221 gatgctttag ataaaaaggt actttcaggt gctaagttca atttagaaga tgcatttgga
    17281 aatatagtag ttaaggattt agttagtgat gaagatggta taattcaatc tagtgtaaaa
    17341 aaaccaggaa catattattt agtggagata atggctccac gtggatatga aaaactgaag
    17401 gataaagtta aggttgaaat tggtaatgaa gatattggta agatagtaga tattggaaat
    17461 atagaaaatc taaaacaaga aaatccacca gtaaatccac caatacctcc agatacagat
    17521 gaaccaatgg taaatccacc agtaccacca agcacagata aaccaaggaa accatcaagt
    17581 tcatctgata cagagaatac tatagttata aatccaccag taccaccatc agaagatata
    17641 ataaatccgc cagtaccacc atcagaagag atgatagaaa ctcctgttaa acagattatt
    17701 ccaataccag aagtagtaaa gccatcagtg tcagaagaaa aaaataataa agttaaagat
    17761 gatactttgg taaatccacc agtaccacca aaaacagggg atagtactac aattattgga
    17821 gaaatacttt tggttatagg agctattgta ggacttatcg tattaagaag aaataaaaat
    17881 acaaattaga ccctaacatt ataaaaaata taagttatat taaaaaatat cttctaaaac
    17941 aatatttaat aatgaaaata ttgttagaag atattttttt ttgaagattt atgtaaaaaa
    18001 ataataactt tttggaaaaa agttccaaag ggatatagac aattaaaaag atataagcta
    18061 taatatagat ataaaattaa atgaggcaaa actagggaaa cctagtcacg caaagctata
    18121 ggggctaaag tttagtaaac catgccagcc agttgccgaa aagagtaaaa atttcttttt
    18181 gtatatacaa agatactctt gggtatcttt tttattttga aatttattac tcccggccta
    18241 actcaaagga gggaataata tgagaccaag taaaaaatta ttaatagcta taatatcaat
    18301 attcttaata agttcagttc cagtaagtgc acatgcagat agtactacta tacaacaaaa
    18361 taaagacaca cttagtcaaa tagtagtttt tccaactgga aattacgata aaaacgaagc
    18421 taatgctatg gtaaatagat tagctaatat agatggaaag tatttaaatg cattaaagca
    18481 aaataactta aaaataaaat tattaagtgg aaagttaaca gatgaaaaag aatatgctta
    18541 tttaaaaggt gtagttccta agggatggga aggtactgga aaaacttggg atgatgttcc
    18601 aggtttaggt ggaagtacag tagctcttag aataggattt agtaacaaag gtaaagggca
    18661 tgatgcaata aatttagaat tacatgaaac agcacatgca atagaccata tagtattaaa
    18721 tgatatatct aaatcagcac aattcaaaca aatatttgct aaagaaggtc gttctttagg
    18781 taatgtaaac tacttaggtg tatatccaga agaatttttt gcagaatcat ttgcatatta
    18841 ctatttaaat caagatacaa atagtaagtt aaaatcagct tgtccacaaa cttatagctt
    18901 tttgcaaaat ttagctaaat agagttttaa taaatttaaa ataaatataa ataaagagat
    18961 gctattataa tagcatctct tttatattgc gtttaaatct aataaatttt gtcttatgta
    19021 taactatgat agattatcaa ttactactcc ttatacaagg cttcaacgtg tttattatgc
    19081 atttgagtaa ttatcgtagg tataatagaa attattgaac caattactat caataaaatt
    19141 cctataacca tgtttatagg gataatttct ttaagtatta ataatgctag tattggagat
    19201 attactggtt taaagaagaa tactaaagat gtaagactag tagatgtagc ttccattgcc
    19261 ataaagtaaa gcgcataacc aacacctgta acaaatacga atacataaat cataatgggc
    19321 atattgtgta aggtatatcc actaaaaata gggatatttg caaatacatg aagcttattt
    19381 ttatttaata tatctgcaat aaaattaatt ttagtgagta aaaccaatat caacatttcc
    19441 aaactaccaa atataaagct aaagcaggtt acaactattc caccaaattt taaggttttt
    19501 cttttaccaa atacagtata cattgaaaaa ataatagcag aagataatgt taagaatatt
    19561 ccactaagag ttagttttgt atgcaatgga tttattataa ctataattcc aagaacttct
    19621 aatataagtg aaattacatt gtgcctatat attttttcct tcaaaattaa gtaagctaaa
    19681 agcattacaa agactggatt acaactgaac aaaacagcaa ctacagatgc ctttgtgttt
    19741 agaacagcaa gttgatacaa tgacatactt actactacac acataaaacc taaaaatgca
    19801 aagtatttta agtctgattt tgtaatagaa agactaagag ctcttaatct ttttacagca
    19861 aaaggtagta agactaaacc gccaacaaaa aatcttgata aagttatttg taagggatta
    19921 aagtcattag tgacagtttt tagactaatt tccattgaac taaaaaataa ggtagtcagt
    19981 attatataaa cataaccttt tttcaagtca aacacctcct tgtaatttat attagtacta
    20041 tagataagaa tattcaagca tattggaata atatggtatg tataaaatat agtacataaa
    20101 attataaact accttaatat agaatgtaag tattctatac taaggtagtt aattatttga
    20161 attactttga tttagttatt ttaaattata taatttggct aatttttcaa atgctggtaa
    20221 agaactttta actttatcat aagatacaca atatgatact ctaaaatgac ctggacaacc
    20281 aaaagaagaa cctggtacta ataataaatt aaattgtttt gcatcattgc agaatttttt
    20341 atcatcttca attggagctt ttgggaacaa atagaatgta ccattaggtt taacacaaga
    20401 atatcccata tcgattaaac tattatatag aaggtcaaaa ttctttttat aaatatttac
    20461 atcaacttct gcatctaaac ttcttgcaat aaccctttga aaaagagaag gagcatttac
    20521 aaatccaagt attctatttg caacatttaa tgaagacata acatcatcaa aatcatccat
    20581 ttgcccatta gcaacaatat atcctatacg ttcaccaggt agagataatg atttactata
    20641 agagtatcct ataaatgaat ttctatagta ttttagtaca caaggaactt tagcaccatc
    20701 ataaattatt tctctatatg gctcatcaga aattaggtat atgcttgtat taaattcttt
    20761 ttgttttgaa tccaataatt ctccaagatt ttttaataat tcttcagaat atatgacacc
    20821 agtaggatta tttggagtgt ttattattaa agctcttgtt tttggagtaa tagcattttt
    20881 tagagcatct aaatctggtt caaaagtctc tatatttgta ggaacttcta ttaattttcc
    20941 atcatagttt tctgtataat ttttatactc accaaaatac ggtgcaaaag caataacttc
    21001 atcaccagga tttaaaagag tttttaaaat aatatttaga ccaccagcag caccacaagt
    21061 cataattaaa ttttctcttg taagatttaa tccatctttt ttatttatgt gttcagcaat
    21121 agcatctctt acatcctcat atcctgagtt gttcatatat ccatgaacta aatttggaga
    21181 ttcttcattt aaaatatcat ttataactgc tttaatagtc tctggaggtt caacacttgg
    21241 atttcctata ctaaaatcaa aaacattttc ctcaccataa atatcagaaa gtttctttcc
    21301 ttcttcaaac atagctctta taacagaact atttgcaact aaagtttgca ttttattaga
    21361 tatcatatgt aacactcctt tttatagatt tatattgctt atgtatatat taccatattt
    21421 ttactgaatt tagacaattt tcgaaaaatt acttgtt
//
LOCUS       DAEMHZ010000056        21052 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000056 DAEMHZ010000000
VERSION     DAEMHZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.056"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..1592
                     /locus_tag="KPK72_002036"
     CDS             168..1592
                     /locus_tag="KPK72_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG2489253.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIDEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1627..3756
                     /locus_tag="KPK72_002037"
     CDS             1627..3756
                     /locus_tag="KPK72_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2489254.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3786..4208
                     /locus_tag="KPK72_002038"
     CDS             3786..4208
                     /locus_tag="KPK72_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2489255.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4227..5378
                     /locus_tag="KPK72_002039"
     CDS             4227..5378
                     /locus_tag="KPK72_002039"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2489256.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5484..5585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5768..6340
                     /locus_tag="KPK72_002040"
     CDS             5768..6340
                     /locus_tag="KPK72_002040"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG2489257.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6404..7054
                     /gene="fsa"
                     /locus_tag="KPK72_002041"
     CDS             6404..7054
                     /gene="fsa"
                     /locus_tag="KPK72_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2489258.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7206..10130
                     /locus_tag="KPK72_002042"
     CDS             7206..10130
                     /locus_tag="KPK72_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2489259.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            10718..11176
                     /locus_tag="KPK72_002043"
     CDS             10718..11176
                     /locus_tag="KPK72_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2489260.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11237..11512
                     /locus_tag="KPK72_002044"
     CDS             11237..11512
                     /locus_tag="KPK72_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2489261.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11571..12845
                     /locus_tag="KPK72_002045"
     CDS             11571..12845
                     /locus_tag="KPK72_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2489262.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            12975..14027
                     /locus_tag="KPK72_002046"
     CDS             12975..14027
                     /locus_tag="KPK72_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2489263.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14027..15058
                     /locus_tag="KPK72_002047"
     CDS             14027..15058
                     /locus_tag="KPK72_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG2489264.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15150..15962
                     /locus_tag="KPK72_002048"
     CDS             15150..15962
                     /locus_tag="KPK72_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2489265.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            15965..16885
                     /locus_tag="KPK72_002049"
     CDS             15965..16885
                     /locus_tag="KPK72_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2489266.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17060..17506
                     /gene="rpiB"
                     /locus_tag="KPK72_002050"
     CDS             17060..17506
                     /gene="rpiB"
                     /locus_tag="KPK72_002050"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2489267.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            17536..18201
                     /locus_tag="KPK72_002051"
     CDS             17536..18201
                     /locus_tag="KPK72_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2489268.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTD
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18311..19813
                     /locus_tag="KPK72_002052"
     CDS             18311..19813
                     /locus_tag="KPK72_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG2489269.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20125..>21052)
                     /locus_tag="KPK72_002053"
     CDS             complement(20125..>21052)
                     /locus_tag="KPK72_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2489270.1"
                     /translation="YAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIE
                     NGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTP
                     LTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNIT
                     KTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKY
                     SMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLS
                     IAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         8515 a         2214 c         3911 g         6412 t
ORIGIN      
        1 actacataaa taaaattaaa caaaaactaa aatgacaaat gtcattatct aaagtgacat
       61 agaaacggtt tcttaaagtt gttactataa aatataaact ctataataaa agtataacaa
      121 aagggaatca tatcgatata gaatataatt caagaggagg taaaattatg agtagttcta
      181 atcaatcgac aataaagaca tctatacagc gagtagggaa atttttaagt ggtatggtaa
      241 tgccaaatat aggagctttt atagcatggg gtcttataac agcattattt ataccaacag
      301 gatggatgcc taatgaaaaa ttgagtacta taggtgaccc aatgataaaa taccttttac
      361 cactattgat agcttatact ggtggtaaag caatagcagg acaaagaggt ggcgtaatag
      421 gtgcagcagc agctatggga gtaattgttg gtgcagatat accaatgttt atcggagcaa
      481 tgataatggg accatttgca ggatgggtaa ttaagaagtt tgataaattt gtagatggaa
      541 agataccaac aggatttgaa atgttagtaa ataacttttc aataggtata ataggtatgc
      601 ttcttgcaat actaggattt tatgcaattg gaccagcaat agtagcaggg acagctctta
      661 tagaatctgg agttcaattt atagttagca aaagtttatt gcctctagtt tcagtattta
      721 tagaacctgg aaaagtatta ttcttaaaca atgcaataaa tcatggtata ttagggccta
      781 taggtatagc tgaggcaaaa gaagcaggta aatctataat gttcttacta gaaagtaacc
      841 ctggaccagg tttaggagtt ctattagcat attggatgtt tagtagagga agtgtaaaac
      901 aatcagcacc aggagctgta ataatacatt tctttggtgg tatccatgaa atttacttcc
      961 catatatttt aatgaatcca gttttaatat tggctacaat ggcaggtgga gcagcaggaa
     1021 tattgacttt ttcaatatta ggagctgggc ttgtagcagc accatcacca ggaagtatat
     1081 ttgcacttat ggcactagct ccaaagggag gattacttcc agtattagca ggagttgcag
     1141 tggcaacagt tgtatcattc ttagtggcag caccatttgt taagagagct tcagcaaatc
     1201 aaagtgaaga agacagtact tctttagaag aagctaaagc aaaaatgagt gatatgaaat
     1261 ctgcaagtaa gaactctgag aaaaatattg acgaaaaaca gcttgaagta aatgagatta
     1321 agaagattgt atttgcatgt gatgcaggaa tgggttcgag tgcaatggga gcttccagat
     1381 ttaaaaatag aataaaaaac ttagacttaa atatagaaat tacaaattca tctgttgata
     1441 atcttccaga tgatacacaa atagtagtaa ctcacaatac attagttgaa agagttgcta
     1501 agaataattc aagtgttgag atagtttcaa taaacaattt tttaaatgac ccaaatttag
     1561 atacattgtt taaaagatta gaatctaaat aaaaataaaa agtaccacta attaggagtg
     1621 gataatttga aaaaaaagaa gattacatct aggcaaaaaa agataatact aatgatagta
     1681 gaaaattcaa agaaaaacat tccaataact atttctgaga ttgcagggac tttagagtta
     1741 agttcaagaa ctgtacttag agatatgtct ggaattgaaa aatggtttga tgaaaatgat
     1801 tttaactttg taaaaaaacc aggagtggga ttgattttag aggaaaatat agaaaatcaa
     1861 aactttataa ttgagttgtt agaagaagaa aaaatagaaa aagaatacag caaggaagaa
     1921 agaaatttaa ttatattatc aaagctacta gtttctaacg agccagtaaa atcctattat
     1981 tttgcaaaaa tattaaaggt atctgaaggg gtactgaata atgattttgc cttagcaagt
     2041 aaatggttgg aaagatttga tattgagctt gttagaaaac cagggcttgg tgtctattta
     2101 aaaggtcaag aaaaaagctt tagagaggca tatgtaaatc ttatatacga ttcattcaat
     2161 gaaaaagaaa tactagatat ggtcagaaat ataagtgaaa atatacaaac agataaagca
     2221 atagaaattt taagtgaaaa tagactttta aatttaatgg acaggtgcat aataagaaaa
     2281 gttgaagcta cattaacaaa gaagctttca gatttagatg taaatttagc tgatagtgca
     2341 tatatagggc ttgtagttca tatttcatta gccttacaga ggataaaaaa tggtgaaaat
     2401 ataactatgg ataaagagtt tctaaaagaa ttatctataa cagaagagtt taaattagct
     2461 aaagaaatcg taaaaggtat ggaaacagat tttaacatgg atataccagt agatgaggtt
     2521 ggatatataa ccatgcatat aagaggtgca aagcaaaggt catcttcaaa ccataaggct
     2581 ttaaacttag atgatataga aataatggaa attacaaata agatgataga tttggcggaa
     2641 gatgaattta aaataagttt aaaaaatgac gagaggttat ttaaagacct agcaaatcac
     2701 ttagggcctt ctataaatag attgaatatg ggattagaaa taagaaatcc tcttttagat
     2761 gaaataaaaa gcaagtattc atatgcctat gatggagttg aaaagatttc tagaattata
     2821 aaagataaac taaatataaa ttcaatacct gaatcagaaa taggatatat tgcaatgcac
     2881 tttgcatcag ccatagaaaa aaatcttatg atgaatacta atataaatat tgttgtggca
     2941 tgtcccactg gaataggtac ttcaagattc ttatcaacta agattgaaaa taaatttcct
     3001 aatttaaata tacttgaaac catatcagct ataaacatag atgaagagta tttaaaggaa
     3061 aaagatgttg atttaatagt gtctacagtt gagttaaata caagtttaaa ttatatatgt
     3121 gtaggtccat ttatgagttt agatgatgaa cagataatca aggaaaaaat aaaatctata
     3181 gctcaaaata aattaatcaa tttaaatgcg aaaaatgata ctaagagtaa gaataaagta
     3241 tatgaacaga taacagaaag tatgaatata ggaaaagata ttcttcaatt tttagaggaa
     3301 attagatttg aaaaatttga aagtaaagat ttaagtgagc ttattgaaga ctcatcaaaa
     3361 atatttgcaa aatctagtga agatataatc tctataaaag aatcgttaaa agaaagatta
     3421 aaaatttcaa ttccatatat agaagaatca aaaattctat tgttacattg tatgagtgaa
     3481 agaatagata ttatgaagtt atcaataata aaactagaga atagaatcgt gctagattca
     3541 aatgaagaaa tcgacaatgt agtttttatg ttacttccta aaaattcacc aagttatcaa
     3601 agacaaataa tgagtgaaat aagtggttca ttaatagaca atataatttt tactaataag
     3661 ataaataaat tctctataga agagatggca ttagaggtaa aagatatagt ttttaatttt
     3721 tatacaaaca gattaaaagc atttatagat aaataaaatt aatattaaaa aggaaggttg
     3781 atactatgag taaagtattg aatgagaata atatattttt agggttagat agtgtttcaa
     3841 aggaagaagc tataacttta gcaggaagaa aattagttga aaatggatat gtaaaagaag
     3901 aatatatacc agcaatgtta gaaagagaaa aagtaatgac tacatatatg ggaatgggag
     3961 ttgctattcc acatggtgta aatgaagcaa aaaaagaaat attatcttct ggaatagtta
     4021 tattacaatt ccctaatggt atagattttg atggagaaaa agcatattta ttaataggaa
     4081 tagctggtgt tggagatgaa catttagaaa tactatctaa tatagcagta gttttagatg
     4141 atgacttaac agaaagacta aaaaattcaa atgacaaaca agcttttatg gaagcttttg
     4201 ccaactaatc ttaggaggga tttaaaatga aaaaggcaat tcagtttgga gcaggaaata
     4261 ttggaagagg ttttattgga ggcttgttag ttaagtcggg ttatcatgta gtatttgctg
     4321 atgtaaatga ggatatatta aactcaataa acaaagataa aaagtatact atacacataa
     4381 gagatgtaga atgtatagat gaagtaattg ataatataag tgcggtttca tctataaaag
     4441 aagaaataat agatgagatt gtacaggctg aaataataac tacagcagtt ggacctttag
     4501 ttttgactaa aatagcctct acaattgcaa aaggtataaa ggctagaaaa gaaaagggat
     4561 taacaagtaa tctaaatata atagcatgtg aaaatgcaat atacgcaagt tcttctttaa
     4621 aagaagaggt acttaagtat ttaaataagg aagaagtaga atatttagag atgtatgttg
     4681 gttttccaaa ctgttcagtt gatagaatag ttcctcctgg taaaaacgaa aatcctcttg
     4741 atgtaacagt tgaaaatttt tatgagtgga atgttgaaaa acaaggattt aaaggggaaa
     4801 taccaactat agttggaatg aatttagctg ataatcttat ggcttatata gaacgtaaat
     4861 tgtttacact gaatacaggt catgctataa cagcttatat aggatattta aaaggatata
     4921 agactataga agagagtata aaagataaat ttatctgtga tatagtcaaa agtgcaatgg
     4981 tagaaagtgg agaaggattg attaaaaaat acaattttga ctcagaagtg cactataaat
     5041 atattgataa aattttaaat agatttaaaa atccatatct taatgatgat gtattaagag
     5101 tcggaaggga accattaaga aaactaagtg ataaagatag attgattaaa cctttaatga
     5161 cagcaaaatc atatggatta tcagttgata atttgattct tggaataggt gcagcgcttc
     5221 attataataa tagtgaagat actcaaagtg tagaattaca agagttgata aagtctattg
     5281 gggtaaaaaa agccgttgct aaaatagcta atataagcaa tgacgaagaa cttttaaata
     5341 atatcgaaaa aagttatata tttatgaaaa atttataaat ttacttgaaa aaaagtagtt
     5401 ctgataaagt aactataata tattattcta cacagtttga attatttgtt gaataataga
     5461 gaattataat atataattaa aaggaataaa ttaatatatt tacctgtata tgtttgataa
     5521 tatggttcaa atgtttctac taagctacca ttaatagctt gactacagga gtgaagaaga
     5581 tatagtttat acttatttta aattatatat taagttaatt actttgaatt ttaagctcct
     5641 gtctatgata tagacaggag cttttttagt attttaatat aaaatactta caagaataga
     5701 tagcaaaatt agaacaaata taattttttt attaatataa ttttagaaag ggctaggtgg
     5761 aagttcaatg aaggctttac aagaaaagat attgagagaa ggtagtgttt caggaaatga
     5821 tatactaaaa gttgatagtt ttttaaacca tcaaattgat gtagcttttt taaatgaaat
     5881 aggaagagaa tttaaagaga gatttaaagg agaaaaggtg gataagatat ttacaataga
     5941 ggcttcagga atagctatag catcaattgt ttcacagtat tttgataatg cacctgtagt
     6001 atttgcaaaa aaatcagaat ctaaaaattt ggataaagat gtatatgaaa ctaatgtata
     6061 ttcatttact aaggcaagag agtattcagt taaagtttct aaaaagtaca taaacaaagg
     6121 cgaaaatata ttgatagtgg atgatttttt ggcaaatgga agagctgctc ttggacttaa
     6181 agatttaatt gagcaagcag aagctaactt agttggagta ggaattgtca ttgaaaaagg
     6241 atttcaagct ggaggagcac ttttaaaggc taatgatgta agattagagt ctttggcagt
     6301 agttgaatct atagataatg gaactgtaaa atttaggtaa tttatgatat tatatacttg
     6361 tgtttagcac atattttaac tatgtaatta tggaggattt tttatgagat tttttataga
     6421 cacagccaat attgaagaaa taaaagaagc aaatgattta ggtgtaatct gtggtgtaac
     6481 tactaatccg tctctaatag ctaaagaagg tagagatttt atagaggtag ttaaagaaat
     6541 aagtgaaata gtagatggac caataagtgc agaagtaata agtttagaac ataaaggtat
     6601 gatagaggaa gcagaaaaat tatctaagat acataaaaat atagttataa agatacctat
     6661 gacagcagaa ggtctaaaag cggtgaaagt actatcaagt aaaggtataa agactaatgt
     6721 aacgcttata ttctctgctg gacaagctct tttagctgct agagctggag ctacatatgt
     6781 aagtcctttt gttggtagat tggatgatat atcacaaaat ggtcttgatt taattgaaga
     6841 aatcgttgat atatttagtg taaatagtat tgaggctcaa ataatagttg ctagtgttag
     6901 aaatccaata catgttttac aagctgctag aatgggagca gatatagcaa cagttcctct
     6961 taaagttata aaccaaatga ttaagcatcc tttgacagac aaaggaatag atagctttat
     7021 gaaggattgg gaaggtgctt ctttaaaatt ataattataa actacgcttt ataagtatat
     7081 taaagaggtt attatggaaa ttctaagtaa taaggctagg ctattttgcc tagtcttatt
     7141 ttggtgtata atatattgaa acatattatt gaaatagatt aattttttaa atgaggaggc
     7201 gaaaaatgga taaaatatta agagagatac aaagagaaga taaaaaaaat ccatatactg
     7261 accaagaact tgctgaaatg ttaaatgtag ctaggtctga agtgatatca gttaggaaaa
     7321 aaaataatat tttagattcg agagaaagaa gaaaaaaaat attaataaaa gatatatcaa
     7381 aaattgtaag tgaaaatcct caaatgtctg aaagaaaaat aactgaaata ttaatagaaa
     7441 atggatataa tatatcgaga agtgcagttt caaaattatt aaaagaggaa aatttaaatc
     7501 agtcaaagat tgggattaag gataaagacc cagcatcatc aggaaagtat gtgaaggtga
     7561 gtaaagttgt agaagaagat ggatttgaag aattgatagg tataaaggga agtttgaaag
     7621 aaaaggtaaa tctagctaag tcagcaataa tgtatcctcc taatggcctt catactataa
     7681 tatatggaga aacaggagta ggtaaaagtg aattagctac atgtatgtat aaatatgcta
     7741 taaaaaacaa cattaaagaa gagaacagcc catttatagt gtttaactgt gcagactatg
     7801 cagaaaatcc taatctattg atagctcaac tctttggggt agtaaaaggt gcatatacag
     7861 gagcagatgc aaatagagaa ggtcttgtag aacaagctaa taatggaata ttgtttttag
     7921 atgaaattca tagattgcct gctgctgggc aagaaatctt attttcatta atagatagag
     7981 gagaatttag aaggttagga gaaagtagtt ctaatagaaa ggcaaatgtt ctcataataa
     8041 gtgctacaac agaaaatcca gattctaatt tacttcaaac atttagaaga cgtataccaa
     8101 tggtaataaa tttacctaat atgagtgaaa gaccaaaatc tgaaagatat gaaataataa
     8161 taaagttttt tgaaagagaa gcaaagcgag taaacaagaa ttttataata actaaagaag
     8221 ttatgtgtgc acttatgaat tataagtgta ctggaaatat aggtcagtta aagagtgata
     8281 tacaagtaac ttgtgctaga gcttttagta aatcgatatt ttctagcgat aaagtaatta
     8341 tagatttaga tagtttaagg gattatataa aaagtggata ttatgattgt aattgtaaaa
     8401 atgaaaagta ttctgagttt tcagttgagg atatatatat agatattagt gaattggtga
     8461 gtggaaaaaa tagaatttta tctgataatg agataagcga gatatataat tatgcagaaa
     8521 aagagcttaa ggttttggaa agtaagtgct tttcagaaga agagttaaaa aatgcattta
     8581 tagaaaagtt agataagaag tttaatgcta ttaaaaacaa taaaaattta tctgatagaa
     8641 gaagtcgaat agactgggga ggacaattaa aagagtctac actggagata atggataagg
     8701 taattgtatt tattaaggag cagtttaagc atgtaaatca aggtctgtac ttggctctag
     8761 caattcatat tgagcatgct ataggtagga taaaagatgg aaaaactata ataaatccga
     8821 gtttagataa aataaaagca tcaatgccaa tggaatatga gttgtcaaga tatataatgg
     8881 gtatagtaga agatttgaca caagtaacat ttccagaaga tgagcttgga tatttggctt
     8941 attatataaa taaattttgt tataatgagg aaagtataaa agataaagtt aaagtagtaa
     9001 tagttacgca tggtaaagtt ggaattgaaa tgtctaaagt tgtaaatcat attttaggta
     9061 tagaatgtac actaggtata gaaatagcac ttacagattc accatcagag gggatagagc
     9121 atgtattaga agagcttcaa aagatagaag ctagaaaagg tattctagtt ctaattgata
     9181 tgggttcttt ggtaattctt ggtgatgagg ttgaaaaaag gcttggaatt agatgtaaaa
     9241 ctgtaaatag agtagataca ttattggcaa tggaagcagg aaaacttgca accattgaag
     9301 ggaaaagttt agatggtata atagctgatt taaagaaaaa taagaattat gctatgataa
     9361 atacaaataa atttagttac agaaaaaacg agtatggaaa gaaaaatgta atcattactt
     9421 tatgtttgag tggtgtaggt acagctttaa atttaaaaga gcatatagaa aaacaaatta
     9481 aggaatatga cactttaatt gaagtaaagc cagttgcttt tttaaataat aatttagaag
     9541 aagagttaaa tgatattgaa gctgaatata atattattgc tatctgtgga actattgata
     9601 tagattataa aaacgtgcct tttatttctt ataatgaggt acttggtaaa aatggtatta
     9661 ataaagtatt agagcattta aatagtaaac aaccagcttt aaatattgaa aaccagttga
     9721 ataatttaat acatgaagat ttgattttat acaattttga aggaatttca aaggagtata
     9781 taattgatac tcttgtttca aaacttgaag aaggtggata tgtcgactca aagtatatat
     9841 taagtgtata taaaagagaa gcaatgggaa gtgttgtaat ggcctctaag gtagccgtgc
     9901 cacatggact tccagaaaac gttataaagc ctgcaatagc aattgcgagg ttgaataaac
     9961 ctatagtatg ggacaataaa tttatggtgg atttggtggt actactggca cttaaagaaa
    10021 ataataagaa agaaatacga agcttatttt caaagattaa tgaccagcat actttagaag
    10081 ttttattaaa tactgaagat aaaaatgaaa taaaagaact tttaagctaa ttgttagtac
    10141 cgagaagaaa gacaaaaaaa tttgctttat tataaaattt aaaactactc aattgctaag
    10201 tagttaatta ctctatataa ggctagttct atgaaattta tttatatgat tttatagaat
    10261 tagctttttt cttggtaaat aaaatatatg aatttattta tgtaaaaagt aaattttatt
    10321 ggacaattct ataaaaattt ttcctatatt tcttataaaa tatagaatat ataaaaaata
    10381 tataaaaata aattaaattc ataataaata attttataaa atagttgtta aacataaaaa
    10441 accatttgta aaaaacaaaa aaatcatttc tctaacaaaa aaaaccacaa aaaatataaa
    10501 aaagtatggc tagttaagtg attaacaagt aaaaattaaa ataaaaatca aaaaaattta
    10561 tcaatgaaag ttagctaaaa tcctggaaga gagcattatt tttgggaaaa cattttcttt
    10621 tgtttcacaa aaaaagccac tttttggcac acaacatgct ttatataaag tcaagaaaaa
    10681 ctaaggtata agacaaaggc aattaggagg tgtaaatatg gagtttataa attttgtaag
    10741 tgataaagtt atagacctta aaatggattg taaaacgaaa gaagatttct ttaaagaaat
    10801 acataataaa gtttttgatt taggctttgt aaaagaagaa tttggagaaa aaatattagc
    10861 aagagaaaat gtatttccaa caggtttaaa tttaggtgat tatggagtag caataccaca
    10921 tacagatgca gaatatatta aagagcaatt tatatcagtc tgtactttta atgaaccagt
    10981 agtatttagt tctatggaag accaagatga aaaagtacct gtaaatttgg ctttcgtatt
    11041 aggacttaat caaccacaca gtcaattatc tgtattaaca gaattaatgg gaattatgca
    11101 aaataaagag ttagttgata agttgatgag ttctactgat aaagaagaag ttttaaagac
    11161 actaaagtct ttataaaaat aaaaaagtac taaaaataaa aaatataaaa ataaaaatta
    11221 ttaggaggaa ataaaaatga aaaaaatatt agtagcatgt ggagcaggaa tagcaacatc
    11281 aacagtagtt tgtgacaagg tagagagatt agttaaggaa aataatatac aagcagaagt
    11341 tatacaatgc aaaatagctg aatgtgcaac aaagcaagaa ggagcagatt taatagtatc
    11401 aacaactata ctaccaacaa cttataatat accagcaata aaagcaacag catatataac
    11461 tggagtaaat acagcagcat tagataaaaa aatattagat gctttaaaat aatcctatca
    11521 tagatacatt gaaataatat tttataaaac agaaaaagga ggatttataa atggataaat
    11581 tattagcagt agtacaatac attttaaata tgggaccgac agtaatacta ccaataacaa
    11641 ttgtagtact aggaatgata tttagaatta agtttacaaa agctttaaag tcaggtctta
    11701 caattggtat aggatttgtg ggtataaact tagttgtaaa cctattaaca tcaacactag
    11761 ggccagcggc tcaaggaatg gttgaaagat tcggacttaa tttaacagta atagatgttg
    11821 gttggccaac aacagcatca gcaacatggg cttcaccagt tgcaccaata ttaataccag
    11881 tttgtttatt agtaaactta ctattattat acttcaataa aacaaagata ttaaacgttg
    11941 atatatggaa ttattggcac tttatagtag caggtgcagt tggatatata gtaacaggaa
    12001 gttatgtttg ggctatattc tgtgcaatac taatggagtt attagtactg tttattgcag
    12061 aaaagacagc aaaaaatgtt caggagtttt atgatttaga aggtatagca ttaccaactg
    12121 gttcaacagc ttcatttgct ccacttggat atttagtagg taaatttgtt gaaaaaatac
    12181 caggaataaa taaaatacaa gcagacccag actctataca aaaaagattt ggtatatttg
    12241 gagaaccaat gatgatgggg attatactag gtataatact tggagcactt gcgggatatg
    12301 atgtagcagg aatattcaat gtaggtatat caatgggtgc agtaatgtta cttatgccta
    12361 gaatggttag aatattaatg gaaggtttaa ttccaatttc agaatcaata agagatttct
    12421 tacaagcaaa atatggagac agagaacttt atataggtct ggatgcagca gtagcaactg
    12481 gacatccagc agttatatca acagctctta tacttgttcc tataactttg ttcttagcag
    12541 ttatactacc aggaaacaaa gttttaccat ttggagactt agcgacaata ccattttata
    12601 tggcatttat agtgtgtttt agaaaaggta atatagttca atcagttata acaggaacaa
    12661 tagtaatagc tatatcactt tatatggcta caaactttgc agatgttcat acacaattat
    12721 tacaacaagc aaatatgatg ccagaaggaa caacaatggt cacaagtata gatacaggtg
    12781 gaaacttgct aaaatgggga atattgaaat tatctcaatt agtttcttca tttggaatat
    12841 tctaatatgt agttaataga gattactctt ttttagtaaa gtatgcgaaa atttaaaatt
    12901 tagcagctac taaaaaagag taatattttt tataaatttt taaaaataca aaatacaaga
    12961 ggaggagatt tatcgtgaaa gcagcagtat tacatggcac aaatgatatg agatttgaag
    13021 atatagaaat aaaaccatgt gaaagtgatg aagtaaaaat aaaagttatg gcagcaggaa
    13081 tatgtgggtc agatcctcca agagtattaa aacattggaa atatccagtt ccagctatac
    13141 caggacatga gttttctgga gtaattgctg aagttggtaa ggatgtaaaa aatgtaaaag
    13201 ttggagatag agtagttgca attcctttta taccttgtaa tgaatgtgaa tattgtaaaa
    13261 gaggattatt ttcattgtgt gatgaccatg gaatgttagg agctaaaagt tttggagctt
    13321 ttgcagagta tgtaaatata aaagctacaa atgtacttcc aataggagat atggactttg
    13381 aagatgctgc aatgatagag ccactagcag ttgctatgca tggagtactt aatataggtg
    13441 ttcaagttgg agacactgta gccgtaatgg gtagtggaac aatggggcag ttggtaatac
    13501 aaggacttaa aatagcaggg gcaggaacaa ttatagcagt tgatatatct gataataaat
    13561 taagagaatc aaaggaatta ggggctgata taataattaa tgctaaagat attaacccag
    13621 tagaaaaaat aaaagaactt actggtggaa aaggtgttga catagctctt gaatgtgcag
    13681 gttcaaaaat aactcaagaa caatgtttac ttataactaa aaagaaaagt aagataggat
    13741 ttttagggat agcatattca gatataactt tatcagaaga agcatttgaa aatatattta
    13801 gaaaagaatt agaattaaaa ggtttttgga actcttattc tgcaccattt ccaggacaag
    13861 aatggactaa gggaatcaat ctagttaatg aaggaaagat aaaattaaaa gaaatggtat
    13921 ctcatagatt ctcacttgaa gatacttaca aagcatttga aatgataaga gatagaaaag
    13981 aagaatttaa caaaatatta attttaccac aaggggttga aaaataatga aagcagtagt
    14041 taaaactaaa ccaggctatg ataatgtaga agttttagaa gtagaagaac caaaagctac
    14101 aggagacaag gttaaaataa aagtagaata tagtggtata tgtggttcag atatacattc
    14161 atttaaaggt gaatatgcaa atataaaagc cccagtaaca ttaggtcatg aattttctgg
    14221 aattgttgta gaagtaggag aagatgttaa aaatataaaa gtaggagata gagttacatc
    14281 agagactact tttgcaactt gtgagaaatg tatatattgt gaaactaaag attataactt
    14341 atgcccaact agaaaaggta ttggtactca agtaaatgga agttttgctg agtatgtatt
    14401 atcaagagaa gaaagcatac atgtacttcc tgaaaatgta tctttattat cagcagccct
    14461 tacagagcca ctagcttgtt gtgttcatgc agctcttgaa aagactacta tagaaagttc
    14521 tgatacagtt ttaataatag gaccaggacc aattggttta ttattagctc aagttgttaa
    14581 atctcaaggt gctacagtta taatgtctgg tgttacaaaa gataaggaaa gattagaaat
    14641 agctaaaaat cttggagtag atagaactgt aaatgtaatg gaagaaagtt tggcagatgt
    14701 agtgaatgaa atgactgatg gaaatggagt aaacaaagga tttgattgtt ctgggtttat
    14761 gccagcagtt aacgaagcct taagactgct tagaaaaaaa ggtactttcg tacaagttgg
    14821 aatctttgct aaaaaattaa atgaaatgga ccaagaagca ataatccaaa gagagttaca
    14881 atatatagga agtagatctc aaaagccaag ttcttggatt acagcattag atttaatggc
    14941 aagtgggaaa gtaaatacag aagctttagt tactagaact gttggtttag acgaatttaa
    15001 agaaggtatt acagctttaa tgaacgggga agaaataaaa gtagcagtaa aatcttaaaa
    15061 aaacatttgt attattttaa aaaaaattgt aaaatctact gtaggataaa aaaatgtaag
    15121 gtcttaaaaa ataacaggag gaagaaagta tgagcactct tagagatcat tctaataata
    15181 ttagaaaaaa cataataaaa atggttgcag aagctaaaag cggacatcca ggtggttcat
    15241 tatcaatagc tgatatgttg acagtattat attttgataa gatgaacgta tgtgcagaaa
    15301 atccaaaaat ggctgataga gatagatttg ttctatcaaa aggtcatgca gcaccagctt
    15361 tatatgctac attagcagaa aaaggatttt tcccagaaga agaattaata acactaagaa
    15421 aatttggttc taagttacaa ggtcacccag acatgaaaaa agtagcagga atcgatatgt
    15481 ctactggttc tcttggtcaa gggctatctg cagcaaatgg tatggcactt gcaggaaaat
    15541 tagataacaa agactataca gtatatacta tattaggtga tggagaaatc caagaaggtc
    15601 aaatatggga agctgcaatg acagcatcac attacaaatt agataattta attgcatttg
    15661 ttgacttaaa tggattacaa atagatggtt ctaatgaaga agtaatgaat gtaaatccag
    15721 tagatgacaa attcaaatct tttggatgga atgtaatagt aataaatgga cattcttttg
    15781 aagaaatagg aaatgcagtt gatgaagcta aaaaagttac aggaaaacca actgtaataa
    15841 tagctaaaac agtaaaaggt aaaggtgttt catttatgga aaataatgct gcatggcatg
    15901 gaacagctcc aaatgcagaa caagcagagc aagcattgaa agatttagaa ggaggtctat
    15961 aggaatggga atagcaacta gagaagctta cggacaagtg ttaaaagaac ttgcagaaaa
    16021 taaagatata gtagtattag atgcagattt aggaaaagct acaaaaagta tatcatttaa
    16081 agaagttgcc ccagatagat tttttgacat ggggatagca gaaggtgaca tgattggtac
    16141 agcagcaggt cttgctactt gtggaaaaat accttttgcg agtacttttg caatatttgc
    16201 agcaggaaga ggctatgagc aaataagaaa ctctgtagca tatccaaatt taaatgtaaa
    16261 aatagcagct actcatgcag gtgtaacagt aggtgaagat ggtggtagcc atcaagctat
    16321 agaggatata tctctaatga gaggtatacc aaatatggtt gtattaaatc cagcagatgc
    16381 tttagaagca agacaagcta ttcttgcatc tattgattat aatggacctg tttatataag
    16441 attaggtaga gctgcaactc ctgatgtaaa cagtgaaaat tataaatttg aaataggaaa
    16501 aggaactgtt ttaagagaag gttctgacat aacagtaata gctacaggaa taatggtagc
    16561 aaaagcctta gaagcagcag aagaacttgc taaagaaggt ataaatgtag aagttgttaa
    16621 tatatctaca ataaaaccat tagatgaagc attaataaaa gaaactgcta agaaaactgg
    16681 aaaagtagtt acagcagagg agcacagtat aataggtggt ttaggttcag ctgtatgtga
    16741 agctttagca gaaactaaag atgtagtagt tagaagaatt ggtgttaaag atgtatttgg
    16801 tcaatcaggt acaccagctg atttattaaa acactatggt ttaactacag aagatatagt
    16861 aaaaaatata aaagagttgt tatagatata aatatatcta aataacaaat tgatttgaca
    16921 taagtcaatt aaagctgtct taaaaataga aagaaatttc tacaatggga cagctttatt
    16981 tttacaatac tttattttta tgataattat aaaatagtct gaaagtaatc aaaaaaatta
    17041 taaaaagagg tgctatttaa tgaaaatatc tattggatgt gaccatggtg gttatgaatt
    17101 aaaggaatat ataaaggaaa atttaataaa taaaggtata gaagttgaag atgtgggtac
    17161 aaattcaaaa gaaagtgtag attttccaat atatgcaaaa aaggtaagtc aaaatgttca
    17221 aagtaaaaaa tctgatttag gcatactttg ttgtggaaca ggaataggta tgtctattgc
    17281 agcaaataag tttaaaggaa tcagagcagc tgttgtttct gactgttttt cagctaaggc
    17341 aactagagaa cacaataata caaatgtact ttgtttagga gaaagagtta ttgggaaagg
    17401 attagcaatg agaattgttg aagaatggtt aaattcaagt tataatggag aaagacatgc
    17461 aagaagatta caaatgattg atgatataga aaaagaaaat ttataaatat ataatttata
    17521 acaaggggga gcgttatgtc tattatttgt gcttcaataa tgtgtgcaga ccaaatgaag
    17581 ttaaaagagg aattagaagc tcttgaagaa gcaggtgtaa aattacttca ttgtgatgtt
    17641 atggatggaa tatttgttaa aaacttggct atgggaccag agttattaaa atcaataagt
    17701 gaaaatacaa caataccttt agacatacac ttagccacag aaactccaga taaatatatt
    17761 gatatgatgt cttatataaa gccaaaatat atttcttttc atgtagaatc aagtacaaat
    17821 gtaaaagctg atatacaaaa gttaagaaat tatggtatag ggccagtgtt agcaataagc
    17881 cctcaaacaa gtgtggataa aatagaagaa tatataagtt tagttgaggg tatcttagta
    17941 atgacagtaa atccagggtt tgcaggtcaa aagtttaatc tttctgtatt ggataagttg
    18001 gataaactta cagatatact aaaagattat gataatcctc catttataga ggttgatgga
    18061 aatataaata aggatactat taaacttatg aatggtaaaa aagtagatat atatgttgtt
    18121 ggtacttcag ctttatttaa tgataagcct ccaatttcat ataaagataa aatagaagaa
    18181 ctaaaagaaa gtataaagta accatgtaat aaaattataa tatgtttctg tttaatagta
    18241 aaaatttagg tggaaacaag aaattttata atatttaata aaaattttgg ttgagtaagg
    18301 agaatttttt atgggagacc tatataaact acaaaatggt actgatatta gaggaatagc
    18361 gtatgaaaat gattctaaag aagttaacct aactgtagaa gaagtaaaaa aaatagctaa
    18421 agcgtttcat atttggttaa aagaaaagac taagaaagat aaagtaactg ttgccatagg
    18481 aactgattct aggataacag gaagccaatt tagaagtact gttattgaaa ctttaactaa
    18541 tgacgattgt aatgtaattg attgtgagat tgccacaact ccagctatgt ttatgacaac
    18601 tattatggat ggatacaatt ctgatggggc gattatgatt acagcaagtc acttacctta
    18661 ttactataat ggtttaaagt ttttcacaga aagtggaggt ttagaaaaaa ctgatataaa
    18721 agagatgttg gatatagctg ttaaaaatga tagtgtagac tatgaggaag taaataaaaa
    18781 aggtgaagtt atagtaaaaa atttaataga agattattct aatttattga tagataaaat
    18841 tagaaaaggt gtaaattcaa gtaaaaatta tgaaaagcct ttttctgggc ttaagatatt
    18901 ggttgatgca ggaaatgggg ctggtggatt ctttgctgaa aaagttttac acatactggg
    18961 agcagatacg acaggtagtc agtttttgaa cccagatggt atgttcccaa atcatatacc
    19021 taatcccgaa aacaaagaag caatggaaag catatgtaaa gctgtgttgg ataataagag
    19081 cgacctagga ataatctttg atacagatgt agacagagct gctattgtag gtaaaaatgg
    19141 caaaccaata aataaaaatg ctcttatagc tgttatatca tcaatagtat tagaagaaca
    19201 tccaaaaaca gctatagtaa cagattcgat tacttctgaa ggacttgcta aatttataaa
    19261 tgaattacaa ggaagacatc atagatttaa aagaggatat aaaaatgtaa taaatgaagc
    19321 tataagattg aatagtgaag gtgaagagtg ccatttagct atagaaacgt ctggacatgc
    19381 tgctttaaaa gaaaattatt tcttagatga tggggcatat ttaatagcta aaattttaat
    19441 aaaagtagca aagctatcct tggaaggaaa gactatagaa gaattgatag aaaaccttga
    19501 agaagctcaa gaagagaaag aagttagaat aggcattaat aaagaagatt ttagaccata
    19561 tgcagaggat atattaaaag atttagattc tcatgttaag agtattgatg gatggtctgt
    19621 agcaccaaat aattttgaag gcataagagt taattgtgat gaatcaaatg gagatggatg
    19681 gtttttactt agaatatcac tacatgaacc attattagct ttaaatattg aatcaaataa
    19741 aattggcgga gcagaacaaa tttattctaa gttaaaactt ttcttagaaa aatatgattt
    19801 aaaaggtctt tagggtaaat agaaagaata tatttatatt gaagaataaa tgtaaatttg
    19861 tacctaagat tacaaatatt acaaaattgt aatcttaggt aacagagatg taactgatta
    19921 tggaaacact atgtgatatt atagatacag agagatgaaa tatgtgatga attatagaga
    19981 aatataattt aaaatgtatt aatatcacac caaaaaggta taatcaaaaa atatgtaaat
    20041 ataaacccca acatttagaa aaagacctct aaggaggttt ttttcttgtt taaataactt
    20101 aaaatatatt tttatattaa tatatcaatt aactcttcta aaagtaagca ttaccttaaa
    20161 taaatcagca tctataatta tttccatgtg tgcattttct aattcaatta tattttgagc
    20221 aattgataaa ccaagaccag agccatctgt ttttctagat atatcacccc ttctaaatct
    20281 ttctattaac tcttctggct ttagatttag tttatcattt gaaatatttt taaacgttaa
    20341 tattatattt tcttccttta taaacatatc tatatagacc ctagtgttag gcatagagta
    20401 ttttagtata ttgcaaatta gattctcaaa aactctccaa gtttttttac catctatcat
    20461 ggtaaaaaca gcatattcag gtgtttctat tataaaatct aaactgctat tttgaatttt
    20521 gtcttcaaat tctacaatac tttgaattaa aagttctctt atatcggttt ttgttatatt
    20581 aaattctaca tctccactac ttagcttaga tgcttcaaat atgtcatcta ttaataattt
    20641 taatctttgt gattttttat ctaaaatatt tatatagtca tttatagtct ctctgtcaaa
    20701 gtcattattt tttaacatat caacatagct taatatagat gtaagtggag tttttaagtc
    20761 atgagatacg tttgtgataa gctctgcctt catcctttca ttttttatat tagcatctac
    20821 agcagtttca agaccgcctg ttatattatt tatacttttg gctaaagtag atatttcatc
    20881 atctcctata acttctaatt tatactttaa attgccattt tctatatttt ttatattttt
    20941 tgatatgtta tttatgtaat taaacttaac agataaaaag ttgtatatat aaaaatgaaa
    21001 aactgtgttt acaagtgtaa ataaaattac tatagctatt gcctcagcat aa
//
LOCUS       DAEMHZ010000057        20803 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000057 DAEMHZ010000000
VERSION     DAEMHZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.057"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..1369
                     /locus_tag="KPK72_002054"
     CDS             83..1369
                     /locus_tag="KPK72_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2489271.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1455..2762
                     /gene="grdB"
                     /locus_tag="KPK72_002055"
     CDS             1455..2762
                     /gene="grdB"
                     /locus_tag="KPK72_002055"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2502..2504,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2489272.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            3040..3771
                     /locus_tag="KPK72_002056"
     CDS             3040..3771
                     /locus_tag="KPK72_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489273.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            3773..5131
                     /locus_tag="KPK72_002057"
     CDS             3773..5131
                     /locus_tag="KPK72_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2489274.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            5170..5655
                     /locus_tag="KPK72_002058"
     CDS             5170..5655
                     /locus_tag="KPK72_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489275.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(5918..6154)
                     /locus_tag="KPK72_002059"
     CDS             complement(5918..6154)
                     /locus_tag="KPK72_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489276.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSGHLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
     gene            complement(6309..6533)
                     /locus_tag="KPK72_002060"
     CDS             complement(6309..6533)
                     /locus_tag="KPK72_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2489277.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(6630..7883)
                     /locus_tag="KPK72_002061"
     CDS             complement(6630..7883)
                     /locus_tag="KPK72_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG2489278.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            8143..9132
                     /locus_tag="KPK72_002062"
     CDS             8143..9132
                     /locus_tag="KPK72_002062"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG2489279.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            9204..9944
                     /locus_tag="KPK72_002063"
     CDS             9204..9944
                     /locus_tag="KPK72_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG2489280.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            10160..11311
                     /locus_tag="KPK72_002064"
     CDS             10160..11311
                     /locus_tag="KPK72_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2489281.1"
                     /translation="MTNLPKQFDSFSEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCSVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            11336..12091
                     /locus_tag="KPK72_002065"
     CDS             11336..12091
                     /locus_tag="KPK72_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG2489282.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNNNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            12283..14670
                     /locus_tag="KPK72_002066"
     CDS             12283..14670
                     /locus_tag="KPK72_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG2489283.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKTNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(14901..15134)
                     /locus_tag="KPK72_002067"
     CDS             complement(14901..15134)
                     /locus_tag="KPK72_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG2489284.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            15423..16013
                     /locus_tag="KPK72_002068"
     CDS             15423..16013
                     /locus_tag="KPK72_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489285.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            16127..16972
                     /locus_tag="KPK72_002069"
     CDS             16127..16972
                     /locus_tag="KPK72_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489286.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            16969..17652
                     /locus_tag="KPK72_002070"
     CDS             16969..17652
                     /locus_tag="KPK72_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489287.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            17649..18365
                     /locus_tag="KPK72_002071"
     CDS             17649..18365
                     /locus_tag="KPK72_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489288.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            18462..18725
                     /locus_tag="KPK72_002072"
     CDS             18462..18725
                     /locus_tag="KPK72_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489289.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            18697..19152
                     /gene="def"
                     /locus_tag="KPK72_002073"
     CDS             18697..19152
                     /gene="def"
                     /locus_tag="KPK72_002073"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2489290.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            19204..19665
                     /locus_tag="KPK72_002074"
     CDS             19204..19665
                     /locus_tag="KPK72_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG2489291.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSVNIMF"
     gene            19715..20617
                     /locus_tag="KPK72_002075"
     CDS             19715..20617
                     /locus_tag="KPK72_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2489292.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
BASE COUNT         8247 a         2183 c         3475 g         6898 t
ORIGIN      
        1 ttggcataga aaatgcttta tatattaata aacgtaataa atacttttaa ttgcgattaa
       61 atatattaag ggggattttc atatgaagct agagctaggg aatatattta taaaaaatgt
      121 tgaatttgga gaaaagacag aagtaaaaga tggagtacta tatattagta gcgaagaaat
      181 agaaaagata gtattagaag atgaaaggat tgtaagtgta aatgttgaac ttgcaagacc
      241 tggcgaatca ataagaatag ctccagttaa agatgtaata gagcctagag ttaaaattgg
      301 agatgaaagc aaaatatttc ctggaattat aaataaagtg aagactgtag gaagtggaag
      361 aacccatgta cttttaggag cttgtgttgt aacttgtgga aatatagttg gatttcaaga
      421 aggcgtaatt gatatgagtg gtcctactgc taaatataca cctttttcaa aaacaaataa
      481 tatatgtatt gttataaaag caaaggatgg tatagatact catgattatg aagaagcagc
      541 tagaattgca ggattaaaaa taggtgcata tgtaggtgag gcaggaagag aagttgagcc
      601 agatgaagtc attgtatatg agacaaagcc attattaaaa caagtaaaag aatatccaga
      661 cctgccaaca gtagcatatg ttcacatgtt acaatcacaa ggacttttac atgacactta
      721 ttactatggg gtggatgcta aacagatagt gcctacattt atgtatccaa ctgaaataat
      781 ggatggagct ataatatctg gaaactgtgt agcaccatgt gataaggtta caacattcca
      841 tcatttaaat aatccagtta tacatgactt atataaaaga catggaaaag atttaaactt
      901 tataggagtt attttgacta atgagaacgt attcttagtc gataaagaga gaagctctga
      961 tatggtagct aaattaatag aatttttagg agttgatgga gttctagtga cagaagaagg
     1021 ttatgggaat ccagatactg acctaatgat gaattgtaga aaatgtagtg aagttggagc
     1081 aaatgtagtt ttgataacgg atgaatttcc tggaaaagat ggcaagagtc aatctatagc
     1141 agacgctact aaggaagcag atgcagttgt ttcttgtggt caaggaaatc tagtggttca
     1201 tttcccagca atggaaaaaa ttataggaac tttagattat gttgaaatga tgataggtgg
     1261 gtataaagga tgcttaaatg aagacggaag catggatgca gagctacaaa ttataatagc
     1321 atcaactata gcaaatggct ataatcattt gacagcaaga tattattaaa taagtatttg
     1381 aatataaagt tctgttgatg taatcattgg tctaattgat gtaataacta taaagtgatt
     1441 tggaggatta agagatgagt aaaatcagag tggttcatta tataaacaat ttctttgctg
     1501 gtataggtgg agaggaaaaa gcagatattc cgccagaaaa aagggctggt gtagtaggac
     1561 ctggaatagc atttcagagt caatttaatg atgatgcaga gataatctca actgtaattt
     1621 gcggagatac gtattttggt gaaaatattg agagtgcaac tcaagaactt ttaaaaatga
     1681 taaaagaaga aagccctgat ttatttattg caggcccagc atttaatgca ggtagatatg
     1741 gagtagcttg tgggactatt tgtaaggcag ttgaagaaga gttaaatatt cctgtaatta
     1801 caggcatgta taaagaaaat ccaggtgtag atatgtttaa attagatttg catatagtgt
     1861 ctacaggaaa ttcagcagca agtcttagaa aggtagttcc aataatgaca aaactaggtc
     1921 ttaaattggt tagaggtgag gaaataggac ctcctgaaga ggaaggatat ataatgagag
     1981 gaataagaaa aaatttcttc catgaactta gagggtctga aagagcaatt gatatgttgg
     2041 ttaaaaagat gaaaggggaa agctttgaaa ctgagtatcc aatgcctgaa tttgatagag
     2101 ttacacctat gaaacctgtt aaagatttat ctaagataaa acttgcccta gtaacttctg
     2161 gagggatagt tccaattgat aatccagaca aaattgaatc ttcaagtgct actaaatatg
     2221 gaatttacga tttaacagat atggattcta tgtcaaataa agaatttaca actatacatg
     2281 gggggtatga cagagcatat gtccttgaaa atccaaattt agttgtacca ctagatgtag
     2341 ttagagagct tgaaaaagaa ggtgtaattg gagaattagt agattatttt ataactacta
     2401 caggaacagg aacgagtgtt ggcaattcta agagatttgg agaagagttt tctaaaaaat
     2461 tgttagaaga tgatgtagat gctgtaattt taacttctac atgaggtact tgtactcgtt
     2521 gtggagcaac gatggtaaaa gagattgaga gagctggtat agcagttgtt catatatgta
     2581 cagtagtacc aatatcactt acaataggag ctaatagaat tgttccagca gtagggatac
     2641 catatccact tggaaatcct atgttaggag aagaggaaag taaaaaaatt agaaagaata
     2701 tagttttaaa agctttaaat gcactaacag aagatataga agaacaaaca gtatttgaat
     2761 aataaaaata caacacaaca ctgaaataaa aaaagatatc aaaaaataat tttttaaaat
     2821 tattttacga tatctttttt ttattaggta aattcgaaat ttgcattttt ttaaattaaa
     2881 tttataaaag tgataaatta gtttattata atattgatac acaaattcaa attagatata
     2941 ctatgaaaag cgagattatt acattttgga acaaatattc aacatattag atcatattaa
     3001 ttgaacagaa aaaattaact ttatataata attagaataa tgataagaaa gaaggtgtat
     3061 atattgattt caattgccat ttgtgatgat gaatcctata tgctaaatca tttaaaggaa
     3121 aatatagaat tatacatgaa gcagaagaat ttattatata atatagagct ttttaataaa
     3181 gtagaagatt tactatgcaa tcatgaaaag tttaatattg tatttttaga tatacagatg
     3241 gaagagatta acggaatgca ggcagctaga aaattaaggt catatggtaa tgagtgcttt
     3301 ataattttcg ttacagtttt aaatgaattt gtatttgatg cttttgaagt agatgctata
     3361 aattatctga taaaacctct atctaatgag aaactatata aaactttgga taaaatagtt
     3421 acaaaacttg atgataatga aagtaattat atcacaatac aaaaaggtta ttcatattgg
     3481 aaattaaaac ttgctgatat tttatattgt gaagttataa aaagaaagat ttatgttcat
     3541 ttaaagaaag agactgtaga ttattatgag actattgaaa atttagagag aagtttacca
     3601 acaaattttt ttaggtgtca tcgtagttat ataatcaatt tacaatatgt atatggatat
     3661 gaaaatggta atgcagttat ggaaagtgga gatagaatac ctgtttctag actacgtcaa
     3721 caaatatttt caaaagttat gcttgagttc atgaaaaata ggaggatata agatgattaa
     3781 tttattttct tccgatttat ttttaaagta tagttttttc ttctctttta ttgtatatag
     3841 aattttaatg gtaatagtgc aatactattt tctaatcagt tttacaaatg ttaattcaaa
     3901 attttggtgt tttattttat attcgataat ttcaattttt ttaatcatta taaatacata
     3961 ttttaattta tataatttat tttcttcaca gattctattt tttcttatga cttttttatt
     4021 tgtaaagtac ataataaaac aatctactgt tttatctata gtatcaactt tgttagtata
     4081 tttaattgaa caattagtat atggatttac agctccatta aattacttga tatttactag
     4141 tgatataaat acagatattt ataaatttga tataaatatg ggactatctt ctttaattac
     4201 tattataata gctggattta tatattggta tagttctaaa aagtttaata ttaaggtcat
     4261 gaactttgat aaatatatag caattttgat gattccattg ttgcttataa tattatttat
     4321 gcaatcattt gaatatagtt caaatataaa tatagataca agtcttggga ttgtaaagct
     4381 gctattaaat acttctataa ctgaagaaat tcaagctttt cttttttcta taattggaac
     4441 tatatgtgta tttttttctc tatttacctt taagaaacta attcaagctc tcgaagatga
     4501 caaggagagg gcaattatga atcaacagat tcatgctcaa aaaaactata ttgaagaagc
     4561 gaaatcacga ttatcacaaa ctatatcctt tagacatgat tttaataatc atttagctat
     4621 tgtcaatgga ttgttgaaaa aagaccaaat tttaaaagca caagattatt taaataagct
     4681 tgaaaaagta acaagtgacc tatcattttc ttgtaataca cagaatattg ttattgatgc
     4741 tcttttcaac aataaattaa gtatagcaaa acaattaggc atagaggtag attgtgaagt
     4801 aattataccc caaagcacta acatgaacga ttttgaccta tgtatagtgt ttgctaatgc
     4861 tattgacaat gcaattaaag cttgtagagt tgtagataaa caaaataggt atttaaagct
     4921 ttccactaag ttagagggtg gattttttat gatagaaata aaaaatagct ataatccaat
     4981 aaataattat acaagtggct caggtatagg tcttttaaat ataaaagaaa ttgctaaaaa
     5041 atatcaaggg gctataagta tagataagac atcagactat tttcaaataa atgtattgct
     5101 agttatttca ttacattgcg ctagtatttg agaacaattt cattgaaaat agtatttgta
     5161 agctatatta tgtacttgaa tagagaaagt ttgatatata gaaatgagga ggttactaaa
     5221 atgaaagttg gaaatataaa tggtcaagca ccaattacaa atggtttaga tagttgtcaa
     5281 agtgaggtac aaggcttaga acaaaagaaa aaatcattac aggatgaaat agagaaagta
     5341 agaagtgata aaagtattga tggtgaaaca gccaaattaa aaatagaagc tttaaaaaaa
     5401 gaaatggaag agattgaaaa taaaattcaa gagttaaaag gaaataaagc aaaaaaaaca
     5461 aataaagaag aaaataatgt ttcagaagta aataataatc tattagagag aaaaaaagaa
     5521 ccttctaata atgaaaatgt aaaacaatta tatgatgttt ttgaaagaag caatgtagac
     5581 gaacaatggg ataatactta tcgtgttctc caagatgata aaaacttaaa agtaaaattt
     5641 aatagaccta tatagtttat ttataaaaat agttaatatt ttaattatca ccacttttta
     5701 gaagaataca cactttattt taaagaccca catatttatt tacagttagc acatttatat
     5761 gtggttttat aactattatt tacaagtcta tattgataac ttttacatag tatataatgt
     5821 ttaaatactt attcaatttt atctatacat aaacaaaaaa agagatttta aaatctcttt
     5881 ttagattgtt gacttatggc tttttgattt aataaaatca agaagccgtt tgaattttat
     5941 atgttggttg atacccttct gatattgaaa caccattttt gtttccaaaa tcgtttaaaa
     6001 ttaagaactt attatgaatg actatttcat tatgaacaat aagcaaatga cctgaatctt
     6061 caatcaatgt tttaacagta gcattaaatg cttgcttata cagttctatc gtttgtttga
     6121 actgcccatt aaagtaaata tgagtcaata acataactat atctacacaa attaatataa
     6181 attttttata ctgtacatta tctaactgtt gattatctgt attacaacat gattgaattt
     6241 aaaaagccat caacacatag aatgttgatg gcctgaaaat agatttaaaa tctctttttt
     6301 attatatatt aattattttt taaatttata ttttttgcat cagattttct aatagctaag
     6361 tcaaaccctc taaatctaat cactataggg tcattaagag gagctatctt tttcacttca
     6421 acttcagtgc catctattag tcctaaggct agaagtctct tcataagttt ttcatttcca
     6481 gctatattgt ctataattcc cttttgtcca agttttaagt tgtaaacagt cattattaac
     6541 acctcctaat gaaaatcatt atcaaacttt atatttataa tatataacaa aaagagcaaa
     6601 ttgtcaatat ataacaaaaa ataaacatac taagttagta tgtttataaa aaatagtata
     6661 catgggatac taatagcatc tagcaaaaaa ccacttacta atggtacaac caaaaaggct
     6721 tttggagaat caccatattt ttgtgtcaaa gaactcatat ttgccaaagc gttcggagta
     6781 gctcctattc catgtccaat caaaccactt attatgatgg cagaatcaaa atcttttcca
     6841 agtactctaa agcaaataaa tattgaaaat aggattataa agattacttg aaaagttact
     6901 attaagagaa taggaggtaa taatgtagat aattcaaata aatctatatt cataagtgat
     6961 aaagttaaaa aaattcccaa tgaaagttct tttaaaaaat ctataagttt aaaatttaat
     7021 tctagtatat ttatcttatc atttaaattt ctaaaaataa cagccgaaaa catacaagtt
     7081 acaacagagg gtaatagtat attaaacgtt atatagaagc atctacttat aatttctcct
     7141 gtatttatac aaataaatat gagaagaacc tgttccaaaa atacagatgg tgttattgtt
     7201 tcggagaagt tattattttt tatcctattc ttatttctaa aaaatttact tccaaatctc
     7261 tttagatttc tattatatct aggaatcgac aagtctatat ttgatgatgg ttttaattta
     7321 tatttattta ttagaaattt agctacagga catcccaata taccacccat aataagtcct
     7381 attgttgcag cagataatcc aaccccaact gctccttcta taccgagatt ttctattgta
     7441 agtccaaatg cagcagaata accatgacca ccttccatag atacatttcc acacataagc
     7501 cctaaaagag gttcaagttt tataatcttt gatattgtaa tagctaatat attttgacaa
     7561 aatgccaata ctccacacaa aatccaatat ctaaataata cactaccccc tttctttata
     7621 agatttatgc ttatttcgag tcctatagtt gtaaaaaaga aggttataaa atttggcatt
     7681 aaatttccac ttatagatat atctgctata ttaaaatatt taaaaaataa atttagaaaa
     7741 caaaataata gacctcctat tactggtgca ggtatacaga aattatttaa aaagttaact
     7801 ttattcttca ataaattacc aattaaaaag aagaatatag atagtgtcaa ggtttgcact
     7861 atatcaaaac ttaaggtaat caaaataagt cctcctttgt aattcaaagt tttaattata
     7921 taacttaaat tcttatgtat tgaaatttta ttatcaaatg tatattaata aatgtatatt
     7981 aaaaagaatc agtagatata attagataat cataaattaa attaaaatat tttaatgaga
     8041 tgtgactttt ttagaattaa aaaccgtagt atattgatat aatagaaata agacttaaaa
     8101 atctgttgat agtattattt ataagtacag taggaggata aaatgtttga aaatttacct
     8161 aagatagatt tacattgcca cttagatgga agtgttagag tagaaaccat gcttgatata
     8221 gctattaaag agaaaataga tttaccttca aataatatgg atgaaataaa aaaattagca
     8281 aaggtttctt ttaattgtac ttcactagat gaatatttag aaaaatttga tttaccctta
     8341 aaagtaatgc aatctaagga aaatttaaaa agaattactt ttgaactttt ggaagatgca
     8401 agtagagaaa atgtaaaata tattgaaatt agatttgcac cattacttca cacccaaaaa
     8461 ggtatgagtg ttaaaaatat aattgagggt ataattgaag gaattagaga agctgaaagt
     8521 atttatgata taaaaggcaa tttaatatta ggttgtatga gaacaatgac ctcaaaagag
     8581 gcattattag taattgaaga agggaaatca tttgtaaata aaggtgttgt agcagtagac
     8641 ttgtgtggtc cagagaaaga gggattttgt aaggaatata aagatgtttt taaattagca
     8701 agagaatatg gatataaagt tacaatacat gcaggagaag ctgcaagtgg agaaaatgta
     8761 ttggatgcta taaatatttt aaaggcggat aggattggtc atggggtaaa aattaaagac
     8821 cataaaaagg cgtataattt agttaaagat aaaaaaatat tgcttgaatt atgtccaact
     8881 agtaatgtac aaactaagac agtagattct tatgaagcac atccatttta tactttttat
     8941 aaagataatt tacatgtaag tataaatact gataatagaa ctgtatcaga tataaattta
     9001 agttcagaac taaatgttat atttgatact tttaaactag gattagaaga ctataaaata
     9061 atttatagaa atgcagtaga agctagtttt gcagataaag aaactaaaga gtatttgaat
     9121 agtttaattt aatagataaa aatatttaaa ctttaaatta agctattaaa ttgacaaaaa
     9181 tacattgtat taggagaaaa aatgtgaaag attatgaaga tgttgtttat attaaactag
     9241 tcaaaaaaga acttgaagaa tggaagaaaa atatattaaa gaaaccttct atatttgaca
     9301 aagcatcaaa gggtatacag aataaattta atggagtatt gccagataaa tatcatgaaa
     9361 taattacttc ttctataaaa gtaatgacaa aaaccgtact gtttggatat aaatatgcta
     9421 caaagccacc acttagaaat atgtcattag aagaaagaga aaaattagtt ttagaaaaag
     9481 ctaaaactta caagaccaca gcaatgattg aaggagcagg aacaggggca ggaggaatcc
     9541 tcttaggttt atctgatttt cctttgcttt taggtattaa agtgaaattt ttatatgatg
     9601 tagcttcaat atatggatat gatgttaaag attatagaga aaggatttat atattaaata
     9661 tattacagtt ggctttttct agtcaacagc atataaacaa aacttttaaa ttgatgaata
     9721 actgggatga atatgtaaat agtctaccag aagacatcaa tgattttgat tggagaacat
     9781 ttcaacaaga gtatagggat tatttagatt tagctaagtt tttacaacta atgcctggaa
     9841 taggagcatt tgtaggattt tatgtgaatg gaaaattaat tgataaatta tgtgaaactg
     9901 ctgtgtctgc ttatagattg aggattcgag agcttaagtt ataagaaaaa ttataacata
     9961 aaaaattagt tatacaatat atctatatgt atctaatttt gtattgataa aaacaattat
    10021 ggataaaagg taaagcatga tagaatctaa tgtagaggtt tgtattttaa tggaaaaatt
    10081 tagtttttat tgcttaagat acaaattgaa ttacgaagag attgaattat gaagagaagt
    10141 aattgggagg aatattttca tgacaaattt gccaaaacaa tttgatagct tcagtgaagc
    10201 tagacaaaaa ggatttataa atgcaaagga acttaaggaa agtgggaaaa agatggttgg
    10261 agttttctgt acatttactc cagttgaaat tccaatggca gctggagcaa ctgtagtagg
    10321 tgtatgcagc gttagtgaag agccaatacc tgatgcagaa agggtgcttc caagaaatct
    10381 ttgtccttta attaaatcaa gctatggaca tgcaataaca gatacctgtc cttatttcta
    10441 tttttctgat ttgttgatag gagaaaccac ttgtgatggt aagaaaaaaa tgtatgaaga
    10501 acttgcaaaa gttaagccaa catatgtaat gcatttgcca aatacttcaa aaggagaatt
    10561 tgcctataaa ctttggaaag atgaaatgat aagattaaaa gaagaggttg agaaatcttt
    10621 aggtgtaact ataactgaag aagatataag aactgctata aaagataaaa atgaagaaag
    10681 agaactttta aaagagtttt atgcacttgg taaattacaa ccatctgcac ttacaggcct
    10741 ggagttacat aatgttttat atcaagcagg ttttaaattt gaccgtgcag aacttaaaca
    10801 ttctttaaga aaagttatag atgatatgaa agaaagatat gaaaaaggtg aatgtcctgt
    10861 tcaaaaagat aagccaagaa tattgataac aggttctcct attggtggaa taagcgaaaa
    10921 aatagttaaa actttagaag atgcaggggc atcagtcgtt gcatatgaac tttgtggagc
    10981 tattagatca aacgatttat tggttgatga agaaatagaa gatgtatatg atgctttaac
    11041 acaaaaatat ataaatatag gttgttcttg tatgatgaat aatgacaatc gtattgaatt
    11101 attagataga ataatagatg aatataatgt agatgctgta atagatgtag ttttacaagc
    11161 atgtcataca tttaatatag aaagttatag aataagagaa tttgttacaa aagaaaaaaa
    11221 taagccattt atgtctcttg aaactgatta ttcaaaatca gatactgaac aattaagaac
    11281 tagatttgaa gcgtttgtag agatgttgta ggaataaata aaagtaggag gaactatgtt
    11341 tagtatagga gttgactcag ggtcagtggc aacaaagggt gtgctatttg atggtgaaaa
    11401 aattattaag aaaataatta taccaactgg atggagccct aaaagtacat ctaaacaggt
    11461 ctatgagttg ctaagtagtg aaattgacaa aaaagatata aaaaaggtgg taggtacagg
    11521 ttatggaagg ggagtaatgg attttgctga taagaaagta acagaaataa cttgtcatac
    11581 tagaggtatt tactttttaa acaataacat aaggactatt ttagatgtag gtggtcaaga
    11641 tagtaaagtt ataaatttag atagagatgg aaatgtattt aactttataa tgaatgataa
    11701 gtgtgccgca ggaactggtc gatttttaga gattacttct aaccttttag gaagcgatat
    11761 tgaaagtata gatacattag ctaaaggata tgaaccagtt aatatatcta gtatgtgtac
    11821 agtttttgct gagtctgaaa ttgtaagtct tctagctcaa aacatttcta ctggagaggt
    11881 agcagcaggg atacttaaat ctattgcaaa taaatccacc tcaatgttag caagaggtga
    11941 ggtaatagat gaagttgcat ttactggagg gcttgcaaaa agtaaagaac tagtaaaaat
    12001 gatagaagaa atactgggta aaaaaatttt tattgcagaa gatactcaaa taataggagc
    12061 tttaggagct gctgtaatag gatttagata aaaacatata aaaagaaaat gagaagctga
    12121 atttattata aataaattca gttttttatt ttattttatt tatttataaa ggaaaacttt
    12181 ggtataattg atttaaatga ttgtatattt gtaaatactt gatatttagt catatataac
    12241 attataattt taaaaattag ctcaaaataa gggggagaaa gcgtgaaaaa atttacttca
    12301 aaaaaagtaa caaggtttgt tgcgctgttt ttaatatcta ttttagtaat gtcaacaatt
    12361 cctgtatcag ctgacaatag caatacattg aatcagataa aaaaagcaga atacagtgag
    12421 acgtataaac aatatctaaa tgatgctaaa aatggacgta cagaaaaata taatgggatt
    12481 ataccaaatc catatcagtt ggaaggtact caaatccaaa gtaaaggaag aacagctcca
    12541 acatcttatg accctagaaa attaggtctt atgacatcaa taaaaaatca agaggattta
    12601 ggaatctgtt gggattttgc tgcaatggca accttagaaa gtttcttaaa gttaaataat
    12661 tatggagact atgatttatc agaagaacac ttaagatggt gggcttcaga tggtgaatat
    12721 ggatggtctg taaatgatat gaatggtgct ttaaattatg aagctatggg atatttaaca
    12781 tcttggtcag gtcctaaatt agaaaaagat atcccataca atggtagagt atctaaagca
    12841 caaggagcaa aaaaaccaac taatatgaat acagcaccta ctgtttttaa tgtgacagat
    12901 gctgtgtgtg taagcaatga cataaattct acaaaaaatg ctatattaca gtatggagct
    12961 gtaacatctg gttattatga agatattaaa tatcaaagtg atgaccaaaa ttcatattat
    13021 tgtccaacaa aaagttttaa tacaaatcat gctatttcaa ttgtaggttg ggatgataac
    13081 tattctaaag ataattttaa tgcaaatatt agaccatcaa aaaatggtgc ttggttaata
    13141 aaaaatagct ggggagatta taattctgag ggtggatatt tctggatatc ttatgaagac
    13201 aagacactta tgtctgatat agataatttt tcaatcaaag gtggtaaaaa aacaaatgat
    13261 gatgagaaga tgtatcaaca tgactatgca agtattgtac ctttaatttc aaaaaagata
    13321 acagctgcaa atgtatttga tttcaataga ggcgatgaaa ctctaaaatc tgtaatgttc
    13381 ttaactgaaa gtataggagc taagtatgaa gtatattatg ctccagtagt aaatggtata
    13441 cctcaagaaa acaatatgaa aaaattaaaa gaaggtactg cccaatattc aggatacata
    13501 acagttccaa ttgattcttt tgatatacca gaaggaaagg gtgcaattgt agtcagtata
    13561 gaggggaaaa atggagagtc aactataggt tctgagtcaa atgtaccagg atatgataca
    13621 tttaaagcaa aagctaattt aggtgaaagt tatatcatag ataatactgg aaaattcttt
    13681 gatataaata gagatagtaa cttctatccg tgtaatttta ctataaaagc tgttacagaa
    13741 aagtcttctg gtgagagtat cccaaatgag tctttaattg gtagtgatag atatgaaact
    13801 gctatcaaag ttagtcaaaa tggtttcaat tcttcagaaa atgtagtttt agtaaatggc
    13861 agttcaatag tagatgcatt ggcagcaact ccttttacat ctgctataaa ttcaccaata
    13921 ttattgacac aaaaagaagc tttaaattca aaaactaaag ctgagataca gagattaggt
    13981 gctaagaaag tatacctaat aggtggagaa aattctataa gtaaagaaat tgaacaacaa
    14041 ttaaaaagct taaatatatc gatagagaga atatcaggaa gtgatagata caagacaagt
    14101 ttattattag ctcaaaagtt aaatggtata aaaaatgtat ctcaaatagc agttgtaaat
    14161 ggagtgaaag gattagctga tgcaattagt gtaggcgctg cagcagcaga aaataacata
    14221 ccaatcatac ttgcaaatga aaagagcgaa ttgcaaggag ctgatgaatt tctaaattca
    14281 ttaaatatag agaaatctta tattattgga ggaacagcat ccctatcaaa caatctagaa
    14341 agtaagttaa aaaatccaac tagattatct ggtagtagta gagacgaaac taattcaaag
    14401 ataatagata atttttataa aaaagatact ttaaaaaatg catttgtagt taaaaatgga
    14461 atcaagaatc aaaatgattt aatagatgga ttggcagttg gtcctttagg agcaaaaact
    14521 gggtcaccag taattttagt tggtgataaa ttagctgata gccaaaaaga agttcttaaa
    14581 aataagactt tagaaaaagt aactcaagtt ggtggaggag caaataaaaa tgcatttaaa
    14641 gagttagtaa aattaaaaag tgctaaatag agtacttaag tattaacata tattagtgtg
    14701 tattagtaac ttatcttaat aaaagctgaa aataattact ttaaattatt gataaataag
    14761 ataatcaatg attgtaaagt tttgttttca gctttaaatt tatgtagtat aagtttaaaa
    14821 acataaaaaa gggttacttt atatagaaat atagaaaatc ttacatttta tatataaata
    14881 acccatattt aattaacttt ttatagtgtt ctaatagata ttattgaact taatggtaaa
    14941 tataatgtgt aagtttcatt taaagttgga ttagattcat ctatatatct tgagcaaagt
    15001 gtaattgata agctatctgc ttctataatt tcaccatata gaggacaact gctaaagtaa
    15061 gaatgtactt caacatattt atttaagttt tcttgaacat aatcgtaaaa acggtttttt
    15121 gtagtagtag ccataaaatc aactccttta ttttttatta aagttgaaac ttttaaaaag
    15181 ataaatatga ttacttctat tagaaactaa gttaaaaaat aagcaaatag atatgaaaat
    15241 tagaataata tattatttat atcttaatta taacactaga taataaaaaa tgctatataa
    15301 aattcagaat tttcgaaagt ttttaagctt atttttaaat atactaataa tactattgac
    15361 attagataga aaaagtatta tattgactat ataaattatt ttagtcaaac tggagggaaa
    15421 agatgcctaa atcatattct gataaagaaa gagagtatat aataaaaaga cttaaagaag
    15481 aagcaagatt gtgtatggag caatatggta ttagaaaaac tactgttgat gagcttgtaa
    15541 aacgggtaaa aataccaaaa ggaacttttt atctattttt tcaatcaaaa gaattgctct
    15601 tttttgaggt attaagagat atacatgact ctatacaaaa agaaatctta tatgagataa
    15661 atagagtaga tgaaagtatt acatgtgaac aattgacaga tatatttatg aaattctata
    15721 ggatggttga tagtacttca attttaaatc tcatgataaa tggagaattt gagatattgg
    15781 tgagaaagtt accagattct ataattgaag aacactttag acatgatgac tttgaaattg
    15841 gagagattat atcttgcata ccaaatgcaa aaaataaaga catagaaagc tttagtggag
    15901 catttagagc agtttttctt accatgctat ataagcgcga ggttggaagt aattgttttg
    15961 aagatgcttt gagattaatg ataaacggat tagttataca acttatggaa tagaatttaa
    16021 attttgtttg ctaataactt ttattaaaaa ttattaacaa acaaaaaaat tatattttat
    16081 tgaccatata gtcaaaatag tcaaaaattt gaaagggtga tattgaatga ttaaagttga
    16141 tgatttatca tttagttata cagatagaga ttttttacaa aatattaact ttgaagtagg
    16201 aaaaggggaa atactaggat ttttaggacc atcaggtgca ggaaaatcta cattacaaaa
    16261 gattctaatt ggaatgataa ctaattatgg aggaagtgtc attgtaaatg gagtagagag
    16321 taaaagacac tcaaataaat tttatgaaaa tataggagtt gattttgaat ttcctagttt
    16381 atatgaaaag ttgactgcaa tagaaaattt aaaatatttt gggtctctat attctaaaaa
    16441 actactatct attgatgagt tattaaaatc agtaggacta gaaaatgaag caaataaaag
    16501 ggtatctgag tattcaaaag gtatgaaatc tcgattaaat tttataaaag ctttattaca
    16561 taatccagat attttatttt tagatgaacc aacaagtggt cttgacccat caaatagcaa
    16621 ggttatgaaa gatataattt tatcagaaaa atctaaaggt aaaactatta ttttaacaac
    16681 tcataacatg ttagatgcaa cagagctttg tgacagagta gcgttcattg taaatggtaa
    16741 aatatctgct ttagatacac cacataatct tattatgtca aaaggagcta ttaaagttag
    16801 atatacttac tttgataatg gagaaaaaac ttctgaatgt tttctgaaca atactgctaa
    16861 tgataaaaat ttgaatatgc ttattgagaa aaataaactt ttatcaatac atagtagtga
    16921 accaacatta aatgatattt ttattgagat aacagggagg aatttacaat gaggctaaaa
    16981 aatcttattt taggagatat aaagtttcaa tttaagtatg gtttttattt tttatatctt
    17041 tttttaagta ttatatatat ttgtattatc aatgtatttc caacttttat gagagaaaaa
    17101 atagctataa ttatgattta ttctgaccca gcagcaatgg gattgttttt tatgggagct
    17161 attgtacttc ttgagaaaag tcaaagggtt ttaaattcct tagctgtatc tccagtaaaa
    17221 gtatctgagt atattttatc aaaggtaatt tcattaggag taatatctag tattgtagct
    17281 atgtttattg ctattacatt aaatttagat aatattataa tatcaactat aggtaccttt
    17341 ttttcttcta ttatattttc tttgcttgga ttgataattg caagtaaagc atctagtttg
    17401 aatcagttta ttgttttaag tattcctatt gagattatat gctttattcc accaatactg
    17461 aatgtacttc ttgacacaaa atcatatgca aatttgtacc cttttaatat atgtatttct
    17521 cttattagtg gagataaaaa ttttataatg attaatattt taattttaat atcaattata
    17581 ataataattt attttataac ttattatttt atttgtagca gttggaaaaa ggtaggtgga
    17641 gtaaaactat gataaagatt ataaatgcgt ttaaacaaga gtttgaacaa attaagagag
    17701 atgctatgct ttttatagta tgtgtttctc caatattatg tggagtattt ataaaatttg
    17761 gaataccatt aatacagaat atatcactta acaagtttta ttatcaatta aatttagagc
    17821 cgtatttctt gatgtttgat ttacttttag cttttattac accttttatg ttcttttttg
    17881 cttctacaat ggtcatattg ggagaaattg atgacagcat ttcaagatat ttaatagtaa
    17941 ctccgcttgg taaaactggt tatcttattt cacgatttgg aataccaggt atcttagcat
    18001 ttattattac tatgattcta cttatctttt tttctctaac taaaatttct ttcttgttaa
    18061 acttatcaat ttctttatta gttttattac aaggagttat tatatcaata ttagtgattt
    18121 ctttatcatc aaataaacta gaaggaatgg taattacgaa attctcaggt gtttttatga
    18181 tgggtatttt agcaccattt tttattttaa ataaagcaca atatattctg ttcttcttac
    18241 caactttttg gatatcgaag gcatttaagg aagataatta tatctatatg tttatttcaa
    18301 ttgttatatc attaatatgg attttattat tatttaaaaa gtttagtaaa aaaatagtta
    18361 aataagaaaa tttaatatat aaatgtaagg ttgacttgac agtaatgttg aaaaatgtta
    18421 catttaagat gtaaagttaa ccttacatga atggagcaaa tatgaaaaat aaaataaaga
    18481 tattaagaga aaaattagga ttgacacaag aacaactagg aagattagtt ggaacatcta
    18541 gacaggctat aaatgctata gaaactggta aaaatgaacc ttcaatatgg ttggcatatg
    18601 atatttcaag aatttttaat gaaccaattg aaagtgtatt tttatttgaa gaaagtgaaa
    18661 gaaaatcaag agcacagata agtaggggag aatattatgg ctataagaaa gataagaaca
    18721 tttgatgatg aaattttaag aaaaaagagt aagtatgtag aaaacgtaga taataagata
    18781 agagaaatat taaatgacat ggcagagacc atgtacaata ctccaaatgg aggaggatta
    18841 gctgcatgcc aagtaggagt attaaagcga ttagttgtta tagatttagg agaaggactt
    18901 attaagcttg ttaatcctga aattattaaa caagaagggg aacaaattgt tgttgaagga
    18961 tgtttaagtt ttccagaagt atggggtaaa ttaaaaagac caaaaaaagt tacagtgcaa
    19021 gctctaaacg aatacggtga gaaaatagaa attaaaggtt caggttttat ggcaaaatgt
    19081 ctttgtcatg agatagacca tttaaatggt atagttttta cagataaaat tattgaacat
    19141 gtaaaattgt aagatagatt ggttattgaa tttataaaat attttatagt aaggagttaa
    19201 attatgagat tatggcataa ggatttaatt gatgtattac ctaaaaatca attagtaagt
    19261 caatggagag agttacttgc tataaaaggt tctatagata aaaaaggtac tccaaaccat
    19321 ctattagtga ataaagtatt gaattatagt atagatgaat ttaaatttta tacaaaaatt
    19381 gtacatgatg aaatgttaaa aagaaattat aaacctaatg aacttaaata tacaagtata
    19441 ttaaaatgga aaaatagaaa ttttgcaaat gatatatcta atgaacatag tttaaattta
    19501 gaaaatttat atgatgattg gcataataaa atgtatttaa agcaatgctt atataattta
    19561 gaagaaaaag caacctgtgg aggaatacca ataaatgaat ggaatatatt actttgtaaa
    19621 tatagtaaag attatgaatt gtggtctgta aatatcatgt tttaattact atagattaac
    19681 attatagtat tattgtatat aaagaggtga ttatttgtat aataaaataa aatatattcc
    19741 aataaattgt gaaatagctt gtaataaatt aaaaaggagt atgccatata aatgggattt
    19801 aaatatttat cgtggatgtg agcatggatg taaatattgt tatgctattt actctcataa
    19861 gtacattaac tccaataatt attttgaaga gatttatgta aaaacaaata tagtagaaaa
    19921 acttgagaga caacttaaaa gcagtaaatg gaagaaagaa gtaatcaata taggaggagt
    19981 tacagatagt tatcaaccaa tagaagctga ctataaaatt atgccagaaa tattaaacct
    20041 gctgataaaa tacaagacac cagctattat ttctacaaag tcagatttaa ttttaagaga
    20101 ttacgattta attgataagc tatctaggat tacatatatc aatgtagcat ctacaattac
    20161 cacagtaaat gaagatacac aaaggcttat tgagccaaat ggtgtggatt caatgagacg
    20221 atttaaaatg ttaaaagaat ttagaaaaac taacgcatca gttggtctac atattatgcc
    20281 aattatacca tatattacag atgattttga taatataaat tcgctattta gacatgcaaa
    20341 agaatgtaat gtacattatg ttttacctgg gactttatac ttaaggggta taactagagg
    20401 tgtttttttt gactttgtta aaaaagagtt tcctgaatta tttgataaat tatcaatact
    20461 atatagtgca ggttcactta atagggagta taaaaatcaa ttgtataaaa tggttaatga
    20521 acttagatat aaatattctc tatcaagcag ttactctaaa ataatgaagg aaaagttaaa
    20581 aaactcagga gatgtacaga tatctttttt tgattagttt tatataaaaa aatttttttt
    20641 actatgatga ttaaaattta ttaaaattaa aatttattga caaatataaa atttaaatat
    20701 ataattaatt cataaaataa aaatatgaaa ggatgatgtt gttcggtgat atttgatatc
    20761 ataaaatcgt tgatataagt attgctggaa aagagtgaat aaa
//
LOCUS       DAEMHZ010000058        20707 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000058 DAEMHZ010000000
VERSION     DAEMHZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.058"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            394..1185
                     /locus_tag="KPK72_002076"
     CDS             394..1185
                     /locus_tag="KPK72_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG2489293.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            1214..2434
                     /locus_tag="KPK72_002077"
     CDS             1214..2434
                     /locus_tag="KPK72_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG2489294.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2454..3149
                     /gene="pxpB"
                     /locus_tag="KPK72_002078"
     CDS             2454..3149
                     /gene="pxpB"
                     /locus_tag="KPK72_002078"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG2489295.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3179..4165
                     /locus_tag="KPK72_002079"
     CDS             3179..4165
                     /locus_tag="KPK72_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG2489296.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4379..4945)
                     /locus_tag="KPK72_002080"
     CDS             complement(4379..4945)
                     /locus_tag="KPK72_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489297.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5297..6364
                     /locus_tag="KPK72_002081"
     CDS             5297..6364
                     /locus_tag="KPK72_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG2489298.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNASLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6383..7543
                     /locus_tag="KPK72_002082"
     CDS             6383..7543
                     /locus_tag="KPK72_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2489299.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7556..9652
                     /locus_tag="KPK72_002083"
                     /pseudo
     CDS             7556..9652
                     /locus_tag="KPK72_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
     gene            complement(9811..10155)
                     /locus_tag="KPK72_002084"
     CDS             complement(9811..10155)
                     /locus_tag="KPK72_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2489300.1"
                     /translation="MEIKRYEGTGRMSRTVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10447..11337
                     /locus_tag="KPK72_002085"
     CDS             10447..11337
                     /locus_tag="KPK72_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2489301.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11555..12841
                     /locus_tag="KPK72_002086"
     CDS             11555..12841
                     /locus_tag="KPK72_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2489302.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12930..13373)
                     /locus_tag="KPK72_002087"
     CDS             complement(12930..13373)
                     /locus_tag="KPK72_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG2489303.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13397..14191)
                     /locus_tag="KPK72_002088"
     CDS             complement(13397..14191)
                     /locus_tag="KPK72_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG2489304.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14757..15233)
                     /locus_tag="KPK72_002089"
     CDS             complement(14757..15233)
                     /locus_tag="KPK72_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2489305.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15334..16503)
                     /locus_tag="KPK72_002090"
     CDS             complement(15334..16503)
                     /locus_tag="KPK72_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2489306.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSTNIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVILHVKNVLSF"
     gene            16666..17529
                     /locus_tag="KPK72_002091"
     CDS             16666..17529
                     /locus_tag="KPK72_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG2489307.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKTYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17600..18193
                     /locus_tag="KPK72_002092"
     CDS             17600..18193
                     /locus_tag="KPK72_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG2489308.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18444..19079)
                     /locus_tag="KPK72_002093"
     CDS             complement(18444..19079)
                     /locus_tag="KPK72_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2489309.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19159..19890)
                     /locus_tag="KPK72_002094"
     CDS             complement(19159..19890)
                     /locus_tag="KPK72_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2489310.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            20221..>20707
                     /locus_tag="KPK72_002095"
     CDS             20221..>20707
                     /locus_tag="KPK72_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide synthetase"
                     /protein_id="HBG2489311.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EK"
BASE COUNT         7904 a         2380 c         3445 g         6978 t
ORIGIN      
        1 aagatatttt acttacaaag aatgataatt atatagatga aagaaacaaa tgaatgaaga
       61 aaaatgattc tgtcttgcat ataaagtgat taaaaatgaa aaatacttta aaaatgaagg
      121 ttaaagccct tattttaagt gaaaataatt aattattaac taaaaaatgg taaaaagatt
      181 ttttgaaaaa gaaatacaaa tgaatgttca attaaaataa ttgcaaaaat attccttaaa
      241 tggtaaaatt atattaatta aatgcaatag aatagttgca aaaataaaat tgaatacaag
      301 tgataaaata tttcatgcaa tatataaaat aaacaaaatt taaaaattaa aactacttat
      361 tctatcaaaa tgttagcaag gaggggtcat aaattgtata aagtagattt aaatagtgat
      421 ttaggagaaa gttttggaac gtataagatt ggtttagatg aagaagtttt aaaatatatt
      481 tcatcagcta atatagcttg tgggtttcat gcaggtgacc ctagtcatat ggaaaaaaca
      541 gtacagctag ctaaaaaaaa tggagtgaaa ataggcgcac atccaggttt cctagattta
      601 ataggatttg gaagacgtga gatgaaaata acaaagcagg aagctaaaga ttatacaaaa
      661 tatcaattgg gtgctttaat ggcatttgca agttcaaatg gatgtaatat acaacatgta
      721 aaaccacatg gagcattata caatatggca gctaaagata aagagttagc tatggggatt
      781 tgtgaagcta tatatgaagt cgataaagat attatactgt tggggctata taatagtgaa
      841 atgataaatt ctgctaaaga aattggactt aggtttgcaa atgaagtatt tgcagataga
      901 gcttatgata ataatggttt tttggttcca agaagtgtag aaggagcagt tatacatgat
      961 acaaaacatg caatagatag ggttgttagg atggtaaaag aaggaactgt agaaacttta
     1021 acaggtgaag ttatacatat caaagcagat tctatatgtg tacatgggga caatccaaaa
     1081 gcaatagagt ttgtcaaaga aataagaaag cgttttgaat tagaaagtat agaagtttgt
     1141 ccattagaaa acatagaagt ttgttcgtca gagaacatag tataagactg ataacatgag
     1201 ggggagataa aatatgagta caaaaaatat tacagataaa actaaaaata aaaaagatgt
     1261 cggagcactt ataggggctg catttataat ggcaacatca gcaattggac cagggttttt
     1321 aactcagact gcacaattta cccaagattt tggaccgaat ttttcatttg taattttaat
     1381 tacaacaatc ctttttatag gggctcaagt aaatgtatgg agagtaattg gtgtatcagg
     1441 acttagaggt caggatatag caaataaaat aataccggga ttaggttatt tagtagcttt
     1501 tttagtagct cttggaggat tagcttttaa tataggaaat gttggtggag ctgcacttgg
     1561 tatgaatgta atgtttaata tgaatatgac attaggaact gtattaagtg gattaatagc
     1621 catatttgta tttatgtcaa aaaattcaaa ttctctagtc gataagataa ctaaattttt
     1681 agcattagga atgataatta tagttggata tgtagcaata tcaaatcatc ctcctgttgg
     1741 agaggcagta tcaagaatgg taaaaccaga aaatccaaaa ggattgatat tcccaataat
     1801 aactctactt ggaggaagtg ttggaggtta tataactttt gctggaggac atagattaat
     1861 agacggtgga ataacaggag aagaaaatat aaaagaaata actaaatctt ctcttttggg
     1921 tatattagtt gccacaatga tgagagttct tttattttta gcaatactgg cagtggtttc
     1981 aaaaggtctt caacttgatc cagaaaatcc agctgcttca gcttttaaat ttagtgcagg
     2041 agcaatagga tataaattct ttggacttgt attatggtct gctgcaatta catcagttat
     2101 aggagctgct tatacatcag tttctttctt aaagacacta aatcctttta tagataaata
     2161 tgaaaaatat tttataatag catttatagc aatatcaaca cttataatgg catttatagg
     2221 aaaaccagct acgttattaa ttttagcagg agctttaaat ggattgatac ttccaatcac
     2281 attaggaatt atgcttatag catctaaaag aaaagatatt gttggagatt acaagcatcc
     2341 tacatggctt ttaatatttg gattaatagt agttttaata tctgcatata caggaataac
     2401 ttctcttagc agcctaggtg ctttatttgc ttaatttaga agaggtgaca tttatggaaa
     2461 ctagatactt gatatcaggg gataaggcag tggttgctga atttggaaat gagatatcag
     2521 aagatataaa caaaaaagta atttcattta tgagagcaat agaaatatcg aatcttaaag
     2581 gtattgtaac agaaatggta ccaacatata ggtcacttat gataagttat aatccattag
     2641 aaatagattt tgatagtttg attgagaatt taaaaaaaat agaagataat ttggaaagta
     2701 tagtattacc aaaacctaaa atacatgaga ttccagtatg ttatgatgag gtttttggta
     2761 tagatattaa aaatgttgca agctataata atctcactgt agatgaagtt ataaaaatac
     2821 acacaagcag agagtacttg atatatatgc tagggtttac tcctggattt ccatatctag
     2881 gtggtatgga tgaaagaata gctacaccta gattagaagt gccaaggact aaaatttatg
     2941 gtgggagtgt tggaatagca ggttcccaga ctggtgtata tcctattgat agtcctggtg
     3001 gatggcaaat aattggtagg acacctttaa aattatatga tgaaaataga gaagaacaga
     3061 ttttgcttag agctggagat tttataaaat ttgttccaat aacattagat gaatttatag
     3121 aaatcgaaaa aaataactta agtatttaaa tatcgttata gtacagggag tggtatatat
     3181 gggattcaaa gttgaattag gaggtttcca aacattaata caagacagag gtagagttgg
     3241 ctatggtcaa tatggtgtat ctggatgtgg agcaatggat gagtatgcac atagagtagg
     3301 aaatatatta gtaggaaatt ctgaagatga ggcttcttta gaagttttaa tgttaggtcc
     3361 tacaattacc tttgatgaat atacgcaaat agcagtaact gggggagatt taggggctaa
     3421 aataaatgga aaagaaattc aaaactggag gtcatatcaa ataaatccag gtgatgtatt
     3481 atcatttaga ggtgtgaaat caggtgctag agcctatgtg tcaatagctg gtggaataga
     3541 tgttccactt gcaatgggaa gtaagtcaac ttatacaaga gctaaaattg gtggatttga
     3601 aggtagagca cttaaaaaag gagattatat aaatactttt attcaggaaa aagattttac
     3661 aataaataaa aaattaagtt caaaatatat tccaacttat agttcagaaa tcgtactaag
     3721 aattgtaaaa ggtcctcaat ttgatgcttt ttctaatggt gaagtagaga aatttttatc
     3781 aaataaatac aaagttacga atgaaataga tagaatgggt tgtagactag atggagagag
     3841 tataaagcac cttaatggag cagatattat atctgatggt atttcttatg gagctataca
     3901 agtacctggt catggaaaac cgattataat gttatcagat agacagacta gtggtggcta
     3961 tactaagata gggaacgtta taagtgtaga tttgtataag ttagcacaag caaagccaaa
     4021 tgatgtagta aaatttgaac ttgtagatat atatgaagca catagactat tgagagagca
     4081 agaagataag attcaagata tctacaagag tatgaaaaat ataagagttg taaaggcaaa
     4141 agtattaaat gatatagcag tttaatttta acaaatacaa tttttattat aatttataat
     4201 ataacttaca aatagctatt tactatttac cataattaag atggtttaga ttagtcaaat
     4261 agctattttt ttagtttgag taaatatttt aaatttaaat aaatacttca aatttaaaat
     4321 aaaagcaata tttaaatcta ttatattatc tacaaaaagt aggtgtgtca tattgagttt
     4381 atttagattt tattggccaa attggaggtg atataactcc atatccacga gctattgtat
     4441 ctttacttgg attgatgatt ttatgtggta cattagcttt tataaatgtt gagtcacctg
     4501 gatttaatct aactaccttt tcacccatac aaatatcaac cacaccactc gttaccacca
     4561 taatttcttc tgatgcatga tatatataat ctttggcatc ctcactttct ggtttaagtt
     4621 caaactctac aaataaaatt gaaggtgtaa actcagttgt aggcattgga gatactatct
     4681 cgtaaaacat ttcactcttt ggaaatttca tcataactct atcttctttt ctaatgacta
     4741 aatcatcagt attattgtca tccataaaca tatataaagg agtgtctaaa acttcagcga
     4801 tttttctcaa tgaagacaag gatggttcaa tagcatctct ttctatttga gagatataac
     4861 ttatagataa atctgtcaat ttggaaacat cacttaatgt ttttttcttg ctttttctta
     4921 tatttctcat cttttcacct agcataagaa cctccaaata ataaaattaa tataaaatat
     4981 tcgcttaaaa caaagtaatt atagcacaac atagaagtag tggcaataag aatacatagt
     5041 agattttgac aattaaaatt taattaaatc caaataaaag tctatgaaaa tgaattattg
     5101 ttaaattaat ataaagaaaa taataataaa aaaatttaat agaagagtat taattgaatt
     5161 aagattataa cattatattt tggaaaagag taaattttca gtaaattatc tcgacatata
     5221 tattgaagta ataatataat ttaaagtata aaattttaaa aattgaatta taacttcaaa
     5281 aaataaggag ggtaatatga agataacaga tatcaaattt gaaaagctta gaattaaatt
     5341 aaaaaaacct gtagtagttt catttggagt aattgaatat ggagaaagtg taattttaaa
     5401 aattgaaact gatgaagggt attgtggatt tggcgaagca gcacctcttg cagcagttac
     5461 aggcgaagtt ttggataatg ttctttcagt attgcttatg tttaaaaaag agttaatagg
     5521 caaagaccca ctagatattg aaaccataca tacaatcatg gatggaatta ttataggtaa
     5581 tacttctgca aaagcagcta ttgacatagc tttatatgat attaaaggaa aaattatgaa
     5641 tgcatccctt tataaagttc ttggaggatt tgatagtaaa gtccaaacgg atattactat
     5701 aagtatagat aagccagaaa aaatggcagt agaagcttta gaaagagtaa aagaaggttt
     5761 taggatattg aagcttaaag caggtataaa tcctgaagac gatattgaag ctgtaaagtt
     5821 aattagagaa gccgttggag agagtataag aattagaatt gatgcaaatc aaggatggaa
     5881 cgtaaatagc agtataaata ctatcaaaaa gttagaagaa tttaatgttg atgcaattga
     5941 acaagcactt ccacattggg atttagatgg tactgcttac ataagaaata aaagtaacac
     6001 aaaaattatg atagatgaat cattacattc accaatagat gcaattaagg ctataaaaaa
     6061 gaatgcagtt gatacattta atattaagct gatgaaatca agtggaatat atcctgctat
     6121 aaaaatcaac aacatagctg aggcaagtgg agtaaattgt atgttaggat gtatgctgga
     6181 aactagaatt ggtataacag cagcagcaaa tttaatagca tctaaaaaga acataacaga
     6241 agctgacctt gatagtttta tgttttgtga agaattagaa ggtatatctg gaggatttgt
     6301 aatggatgga gatattatga atcttgtcaa taaaccaggt ttaggaatag aagtaaactt
     6361 atgataaatt tggaggttaa atatgaatac acttttttca aatccaggta gtatacttgc
     6421 tgtaatgaca tcaatgattg cattgggttt ttacttacaa agatacaaag ccattaaaag
     6481 cttaggacca gcacttacaa taatcataat gggaataata ctttcaaact taaaagtagt
     6541 tccagtaagt actgagttat atggaactat ttcaacatat gctattccag tttctatgac
     6601 tataatgttg atgagtgttg acttaaaaga aatgacaaaa ttatcaagag aacctttaat
     6661 tgcaattttt gtagctgtat taactgtaag tatcatggca tttttatttg gattagtatt
     6721 tgcagaaaaa atatcagaag gttggaaggt tgctggaatg tttgtaggta catatactgg
     6781 tggaagtgct aatcttacag ctattggaac aggtcttaat gtaagtagac aaactcttgc
     6841 tgcagcaaat gcagcagact atgtaattgg agtaccaaca ttgatattta tgtttgctct
     6901 accagcaata ttaaaaaatt caaagaagtt taaaaaactt tggccatatc atgtagaaga
     6961 atcagaatta gaggattgtc aaaatgaaga atttatggag tcaaaagaat ggagtattaa
     7021 agatatagct tggatgttgg ctataggttt tgttgttaca gaagtttcaa caatacttgc
     7081 tggatatttc aattcaagtt ttagtagtgc agcaagaata cttttagtta caacaatttc
     7141 aattataatt gctcaattaa agccagtaaa gaaactaaag ggaaatttag atttaggatt
     7201 atttgttgca ttattctttt tatgtacaat aggtttttca gtagatataa aagagttttt
     7261 aggttcaact tttactataa cactttactg ttttagcata atttttgctt catttgtttt
     7321 ccatttaggc ataactagac ttttaaagat aaagtatcaa tatgtaatac tttctatagt
     7381 aggagcaatt gctgatggtc ctacttcagc tttagtagct gcttctgcaa agtggaattc
     7441 gcttgtaagt gtagcagtcg taatgggagt aattggtgga gtattaggaa attatgcagg
     7501 tatatcagtg gcatatgcaa taaagatgtc tctaggatta taggggggag tttaattgat
     7561 aaaatttaaa ttatacatat ctggactata cagtggaaat gtaatctttg atggagattt
     7621 attaattgaa aagttgaatc cttttacaaa taaaatagaa agtttaaaac caataagcaa
     7681 agaagaaaat acatactatt taaatcttac aaaaatagat ttaaagtctt tatttaataa
     7741 ctttgatgta tatactaaaa gcttagtaaa cacagataaa gacacagtta taaataactt
     7801 aggagaaacg cattctaaac ttaatgaata tatatgggtt caaagaaaca aaaagttccc
     7861 actagatata attattgtag ataataaaat agtaggtttt atctgtttat ccagagaaac
     7921 ttgtactata ttaattatgg atggatatga agagtatact gtattaaaag aatgggaaaa
     7981 aactcataaa aatgaagaga tatattctat aagatttggt ggaaattata tgattaatat
     8041 gaaagatggt ataaaacttt ctacagatgt atatctccct gattttgtag attctaccaa
     8101 aaaagcacca acaattctta tgagaactcc atatggaaaa gaaaatgata aagaaatata
     8161 ttataaatat gtacaaagag gatatgctgt tgtaatataa gatgttaggg gaagaaatga
     8221 atcagaaggt aagtgggaac ctatgattca tgagagggaa gatggagata gtaccataaa
     8281 ttggatagta tcacaggaat ggtcaagtgg aatagttgga atgcttggag catcatatct
     8341 tggttatgtc caatgggcag ctgcatcttc aggcaataag catcttaaag ctttagttag
     8401 tatagtaaca tcaggaagcc cttttattga tataccaaga aaaggaggag cttttgtatc
     8461 tggtatgctt gcatgggcat ttatggtatc aagaaataaa gttgatagaa gcaaaatggt
     8521 aagagatgat tgggatgatg ttttaaacat acgaccaata gaaaatatac ctgttgaagc
     8581 tcttggatat agaattgaat ttctggaaga atggttaaag agagtagaaa aagatgagta
     8641 ttgggatttg atggattggc atttacaaaa agataaaata aatgtacctg cattggttgt
     8701 atctgggtgg tatgatgata atagtatggg aactacagag gccttagatg ttataaagga
     8761 ctatgaaaaa ggaaagagaa aggctattct aggtccatgg atgcataatt caaatacttt
     8821 aagagatata aatggtattt ctttaggaaa tagttcatta agatatgatt tggattataa
     8881 ttatctatta tggtttgata aatacttaaa aggtatagaa aataacatag atacgactgc
     8941 tccagtagag tattactctg taggttctaa taagtggaag acagaagaaa attggccaat
     9001 aataaataag atagataaaa gtatgtatct aataagtgat ggaaatgcta acacatcttt
     9061 aggaaatggt agattagtat ttgataatga tttagaagaa aagtatgata gttatatata
     9121 taatccaaaa gacccatcag tacaactaat tgatatgtca gagaatgaag ttggagttcc
     9181 aaataactac aaagatttag aaaaaagaag tgatatgttg tgttatacat cagatgcttt
     9241 tagtgaagaa tttactgtaa caggtgatat aaagttagag ttttttgcat caagttctgc
     9301 caaagatact gactgggtta taaaaattat ggacgtggat ttagatggaa attcaattaa
     9361 attagctgat ggtatactta gtgctagatt tagaaatagt ttttataagt cagagtttat
     9421 ggaagaagga gagatttata agtttacggt tataacttca aaaatatcaa acacgtttaa
     9481 agttggtcat aaaattagac tagatataac atctagtgct aaaaacttta ttttccaaaa
     9541 tagcaacacg acagagggat ataatagtat tgaatatata gaagcaaaga atacgattta
     9601 ccatggaggt aaatatccat ctaaattgat attgccaata gagaataagt aaatttatat
     9661 agattaaata gtgaggaggt gtctcaaaat gaatttttta gttcgtgaga cactttcttt
     9721 tatacgaaat caaatatatt ttcatcattt caacaatgaa aaagaggctt atctctctgt
     9781 tttgagatag cctcctataa gctaaattga ttattttgta gctgcaacaa cgctcatttc
     9841 aactaacaaa tgctctctag caagtctagc ttcaacacaa gctctagcag gttcaaaccc
     9901 tttttcaacc caagcatccc aaacagagtt catagcttca aagtctttca tatctcttaa
     9961 ataaatagta acagatagca aatgttgctt atctgaacca tatttgttta ataaatcttc
    10021 tattttagct aaaacttcag ttgtttgttc aattactcct ccttcagcat gagtttgtcc
    10081 acataaataa actgtattgt tatgaactac agttctactc attcttccag ttccttcata
    10141 tctctttatt tccatatttt aaatacctcc atagttaaaa aatctattaa aaatctaaat
    10201 aataaattaa aaaaagtcta tactatataa aattatatta tatattctga taataatcca
    10261 taaaaacata taacttttta gctataatta ttattgttat taattgaaaa gatttattag
    10321 ttacttgaca tagtttacac ttttaattat agtataaatt ggaatattta gataattatt
    10381 atttaatgta taagctaagt ttttaattaa tatggcttta tacatttata tagaaaagag
    10441 gtacgtatga gtagcacaat caaatcaaat cgaataaaag gtataatgtt tataatagca
    10501 tcagcgcttg gctttgcaat gatgtctgca ttcgtaaagc tagctggaga tttaccaagt
    10561 tttcaaaaag tgttctttag aaatctggta tcagctataa tagctttttg gcttatcata
    10621 aaacataaag gaagtctaac tggtaaaaaa gaaaatttaa aagtactttt atataggtca
    10681 atatttggaa ctctaggagt tatatttaac tactatgcaa ttgacaagtt gatgctttca
    10741 gatgctaata tgctaaataa aatgagtccc ttccttgtag taatattttg tgcaattgct
    10801 ctaaaagaaa aaataaatgt aaagcagata ggagctatta taattgcatt tataggagct
    10861 ttattcataa taaaaccaac atttagtgta gaagtaattc catatcttgg aggaattgca
    10921 ggagctgtat ttgcagcaat ggcatatact tgtttaagag ttcttggaga tagagaagat
    10981 tactatacaa ttgtattttt cttttcagtt ttttcattag ttactgtagg gccaattgct
    11041 tttgcagtat atgaaccaat gacccttatg caacttatat atctattact aggtggagta
    11101 tttgcaagtc taggtcaatt tggaataact ctagcatata aatatgcacc tgctaaggaa
    11161 atctctatat ttgactatag taatataatc ttttcagcaa ttttaagtat attcttattc
    11221 aatgtttatc cagatatgtt aagtgttgtt ggatatttaa tagtattttc ggcagcattt
    11281 tatatgtttt tatacaataa gaagttagat aaattagaca aagaaaagaa gaagtaaact
    11341 agataaaagt gtttttataa tttgtattga atatttaagt ctttgtatta aaattaaaga
    11401 aaatttaatt aaaaatgttt ggtatattaa cttataacag cagttaaaat tgaaaaatta
    11461 taactgctgt ttcttttatt aaaaatatcc aaatgtctgt aaaaatagac tataatagag
    11521 tagtataaat aatataaagg ggataaatta aagtatggaa aaagtgaaag ttgctgtgat
    11581 tggagcaggt aatagaggaa cgtatgcata tgctccatat atttatgaaa attctgatgt
    11641 atgtgaaata gttgctgtag cagaacctaa aaaaggaaga cgagaactat ttacacaaaa
    11701 atataaccta gatagtaaaa atgtatttga aactttagaa gactttttta aacatgacaa
    11761 gatggcagat gcagtaataa tagctacaaa tgatgataga cattatgatg tagcaaaact
    11821 agctcttgaa aaaggttatc atgtactatt agaaaagcca atgtctaata gtttagatgg
    11881 tttggtacat atagatgact tgtgtgataa gtataaagat aagattttta tgatttgcca
    11941 tgtactgaga tattctccat tttataataa attaaaagaa attgtagaaa gcaaaaaatt
    12001 aggagagcta gttagtatac aatataatga aaatatagga tattggcatt ttgcacatag
    12061 ctttactaga gggaattgga gaaatagcaa tgaaaccagc cctttaattt tagcaaagag
    12121 ttgtcatgac atggatattt tgttatattt agtaggaagt agatgcaaaa aaatatctgc
    12181 atttggaagc ttaaagcatc ttaataacca aaatgctagt ggagagatgg cacaaaattg
    12241 tcttcagtgt ttagttgaaa aaaaatgtcc atattcagca aagagaattt atttagaaaa
    12301 agatagaagt ataaatagag ctgttcacat aaatccaact gaagagaatc tattaaatat
    12361 acttaagaca agtccatatg gaagatgtgt ttacagatgt gataataatg ttgtagacaa
    12421 tatggtaaat atactacagt ttgaaaatgg agtgacggct acttttaatt tatgtgcatt
    12481 tactaaagaa aatggaagaa ctataaagtt aatgtttagc catggtgaag ttggtgggga
    12541 tttaaataaa aatgagataa gaattaaaga atttggaaaa aacgaagaaa tagttatgaa
    12601 tccatcaaat caacagaata tggtagagga atatgataga aatcttatag ctgaatttat
    12661 taaattagtt tcaaataaag aactagaaaa gggtagagta gcagcaaaag aagctataca
    12721 aagtcatgta atggcatttg cagcagaata ttctagggtc agtgatgaag tagtgtatat
    12781 agaagaattt tttgatagtg ctaagcaaat gactaaggag atagaagaaa ctatatttta
    12841 attaataaaa atttattaaa taaaataaag atagattaaa taatagaaat acgaaaatat
    12901 gtgttctatt aaggaatcta tcttttttat taaggttttt tcttatccct atcaaaaaaa
    12961 tccatacatt ctgataaaag ataagaaagt aaagcgaata agatttctgt acttaaagga
    13021 atcgttactg aatccataaa ggtatcaatt atgttcatag ctagaaaagt tagtataaaa
    13081 tcaattataa attcgcaaag tttatatatt gagtatggta atttgtttac atatctgaat
    13141 atatctaaaa tagtagtgca tagaaaatca attggagtgg ttatacaaaa atatattaaa
    13201 aagaaaatta atacagctcc aagagattta tattcaaatc ctaagaatga taagaagcca
    13261 attcctataa ccgtgaaaat acaaaaagtt ataccaaaaa aaacagcaaa aaataaaaat
    13321 acagaaataa attcgcctaa aggaatcctt cttttattac ttatcttaat caactcctta
    13381 atttatgata tgaaatttat ataaataata acattcttaa aaattcataa tagtcctctg
    13441 tttcaaatag tactgtttga gaatctattt ttttcatatt tggataaaaa gaggccttaa
    13501 aagctttata atgttctcta aattttattt ctatcttaaa tttatctggc aattttatta
    13561 agtgcctaga taaatctcca gataaagctt ttttgacacc tttttcaatt ttttttagtg
    13621 caaggtttgg atggattgaa attgaaccat ttccaattcc ttttgacaca ggaacagtga
    13681 ctatatttgg atttagtttt tttgcactct cacaaagcat ttcatctcca cttaaaaatg
    13741 ctacaggaac attataatat gcagcaatat aagcatttat agtaaattca gatgctattt
    13801 catcatttat tttaaaatag tcataatcaa tatccatagt atgagaaagt ggattaccat
    13861 tacaagtagc tccagagtga tagccggtga atatacatgc atcaaagctt tcatcaattc
    13921 ctgccatcat aactagtggg tctttggtcc atcctctaag tattttgacg ttttcaggta
    13981 aaatatttgg attaatattt ctaccactgt catgtgcatc tttaacaagt atttcatgta
    14041 caccagattg agtagctcca tcacatgctg ctgttatctc tttactcatt tgtgttctat
    14101 agtgttgcga aaattcacat tcaagttttg tttcatccca atttacaata ccacaagtac
    14161 cttctatatc agcacttaaa tacaatttca taatatatac ccccttatgt ttattattga
    14221 tgataataac agataacaga aaatacataa tcaataaagg gcataaaaat attatgtctt
    14281 actattaact attattaaat ctgtaactga aaaataacta aattggtata aaaaaatata
    14341 acactatgtg tcatttattt atatagtttt ttatatttga tgtttatata aaattgtaac
    14401 atataagtgg ttacatttac tagtttaaca tagttaaaat agttaggtat tatttttaag
    14461 ttaaattgat tattataaaa tcgcagaata aaagaaaaat ttaatttatt aaaatgcatg
    14521 aaatgttttt tcctcaacat aatgctaaat taaactcaaa aagaaataat agaaaaatct
    14581 tatgtagctt agaaagaatg tgaatgattt aagggaaaaa tagaaattta ttatataata
    14641 ttaggctgta ttatataata cagcctaata ttatatgtat atgtgacata aaatcttggt
    14701 aagtgtaaat aaaataaatg tggaaaacaa gttaatgaaa tcaagaaaaa agtaatttaa
    14761 aattcaattt catcttccca attaaattct cctgattttt ctacactctt aggccagtga
    14821 ctacaccatt taggaacgga cggattttca accctatcaa cgcttggagt atatggcttt
    14881 aagtcaataa caggactatt atcattagca tcaatatatg gtatttttat tatgccattt
    14941 tcatggtcga tattgataat ttgtacagca gtcaatgcta ttggattggg tcttataggt
    15001 gaccttgttg caaatattcc tataacgtct ggtccacttt tataaggttt tggggtttct
    15061 aaaatacttc ttagttcagg attatcaaac ccatcagccc accaaaatac atttaaatga
    15121 ctgaaatcgt cgagttcatt taatgcaggt acatactttt tgtctaattt aataaagacc
    15181 tcttcgcctt gaatacatat tttacctata ggatttatta atatttcttt cataatatta
    15241 cctccaacta tttaatttat catttgtaat tttagggtat aacatgacat catgtgaggg
    15301 tcaatataaa ataataaata aaaatattaa tagttagaat gataatacat tttttacatg
    15361 gagtatgaca ccagtaataa ggcaatcttc gtcaatatca aaagtagagg tatgcaaagg
    15421 ggaagtaatg tttttttcac gatttgcaca accaaggtgg taaaaagctc cttcacagtg
    15481 tcttagaaaa tatgaaaagt cttcagcccc aagagtgggc gagtctttaa ggataatatt
    15541 ttcttctcca aacaattcta cagcattttg tttgacgtaa tctactagat ggtttgaatt
    15601 gataagagct ggataacctt cctcaatttc aacagaaata cttcctccaa atgttttaca
    15661 agtaaaatcg caaatttctc taattcgttt ttttgaaaac tctctagttt caggagtcaa
    15721 ggttcttaaa gtacccctta taaccacttt atcacaaact atattttcct tgattccacc
    15781 ttcaatcttt cctagagaaa taaccacaga atcagttgga tttgtgtttc gactaacgat
    15841 tgtctgtaga gctgtaataa tatgagcaga tataacaata gcatcaatac cttgatgtgg
    15901 ataagctcca tgacattttg aaccatgtac agttaattga agtgtatcgg tggaagcatt
    15961 tagagtgttg tattttaatt caattagatt tttgttgata ttgggattta catgaagtcc
    16021 aaatatatat tttacatttg ggttttcaag acatccatct tctatcataa gttgagcccc
    16081 accaatagtt tcctcggcag gttgaaaaaa gaattttaca tttacattta agtaatcctt
    16141 taatttaaat agtatattac aagtacccag taaaatagca gtgtgtgcat catgaccaca
    16201 tgcatgcatt ttgccaatat tagtagattt ataagaataa ttaagttctt ctgttatagg
    16261 taaggcatct atatcagctc ttatagcaac tgtttcaaag tttggactca cgttaatgta
    16321 tgcagttatc cctgtatgat tcttatattc aatataattt atacctattt catttaagta
    16381 tttaataatt tttttcttag tttgaaattc ttctaatcca agttctggag tcttatgtaa
    16441 atctcttctt atatttataa cccaatcatt tatctcatga caaagagttt ttatattaag
    16501 cataaaacac atcctaacta tattaaaaat ttatttttct tcattaatta tattatttta
    16561 tgtaaatcat tattatgtat caagtacaaa taaaatatct tataattata ttagagatat
    16621 ttagtatgga gaagaatata tcattttata agaagaggtg tttaaatgtt aggaataatt
    16681 gcgccatctg ggccattaag aaatacaagt ttggaggaaa taaagtttaa tttagaaagt
    16741 tatggttacg aagttaagtt ttctgaaagt tgtagtttaa attataaagg ttatttagct
    16801 ggtaatgatg atattagagc aagggatata gaagatatgt ttttagataa agatgtagat
    16861 ataattatgt gcttaagagg aggttatgga acaacaagaa tattggataa aataaattat
    16921 gatataataa agcaaaatcc aaaaccattt ataggttttt ctgatataac aggtttaaat
    16981 ttagctttct ataaaaattg tggtctttta ccatatcatg gaataatggc agcaaatgta
    17041 ggtaaatggg ataaatttac ctataaatca ttggtaaatg ctttagaatt taaagatgaa
    17101 ttatatttag aaaatccaaa agaagaaaag acttatactg tatgtgaagg taaagcagag
    17161 ggaataataa tgggaggaaa tttatcctta ataattgcca caatgggaac aaaatatgaa
    17221 atagatgcaa aagataaaat tttgtttatt gaggaaattg gagaacctca atataaattg
    17281 gatagaatgt taacacaact atattcttca ggaaaacttg aggaatgtaa tggtataatt
    17341 tttggagatt ttaaggattg tattgaagaa aatgatttga tggaattact aatagaattt
    17401 gcaaataagg tcaataaacc ttctatatat aatcttcagt caggacattg tatcccaatg
    17461 ataactattc ctttgggcag aatgtgcgaa ttagatgcta cagaaaaaat cgttaaatta
    17521 aaaaaatagt gtgtatataa atataataga aatatggtat aatcttatac aagtaaaaat
    17581 ttagctagga ggatgaaaaa tgtctgatga tagaataata gactttaacg aactaaagaa
    17641 caaagttaaa gattctgatg tagataaatt tgagcaatac atatataact tatatttttc
    17701 tgttatggat ggaaagatgt ctatggctga gttttcaaga aaaatattcg attatatgag
    17761 agataacaac atatctcaag aaaaatttat gaaaatacaa aagcaattta tggagagata
    17821 tggtatggac actgaagaag tagaaaaaca acttagaaac tttggaatcg acccttctac
    17881 agcaggtttt atgagtaata atacttccag caaagtatct acagaagatt tagagtcatt
    17941 taagaagagt gcaggatttt atgagaaata tggagaaaaa atccaaccaa agagttgtat
    18001 aacaacattt ataaaaaatg acttaaatga tattaatgta ataatcgacc aagaaaagat
    18061 aatgctttgt agtgatagaa aaataaatct tatggattca gaactaaatg agtttttatt
    18121 agaatataag aatatgttta ataaaaaaat aaaggtagta atgtgcgaaa ctacgaataa
    18181 gtatgattat taaaaaatga atttaaatac tgaagtagta tcaattaatt gtactactta
    18241 aaaataaaaa tcgcaattta ttattcctta taaatttgta taaggaatat ctaaactgcg
    18301 attttttata ttgtgaagat tagtaataat agatagtgtt ttaaattgta atcttctcac
    18361 taaaactgtg tttttctatt agaaatttta ttaaaaataa atatctgata attgtattat
    18421 atgggatgta aagtattaga ggcttaacga atctctttta atacattaac tccattttga
    18481 atcatactgt tgaaaaatac taagtttaaa atctcagcag ggtgaacgtt aggaatatca
    18541 taagtatcta ctaaattcat aggtacaact atattttttt caatattatg ttgattaaaa
    18601 tatgatttta gagttattgc aaattgatat atacatatat ctgtgcaatc gccaactata
    18661 attatattat ctatattatc aaaatccaaa ttttctaatg caaaaaaacc attagtagaa
    18721 tttttaggaa gaatttgtag gttttctatc gacttaagtt catcaaccaa ttcactttct
    18781 acatcatttt ccaaacagtg aacaggataa ctcaaaagtt ctattgattt aggagtatga
    18841 gaatcagtaa atgctattac tttatttaat ttgtttgata ttttttttgt aaacatttca
    18901 attggcttta ttaaagactc tactctagga gagtataatg caccttgtct tgcaaagcca
    18961 ttattaacat caattataaa taaagcagtt ttactcaaat cataattttc tattttttct
    19021 attggtagat tatctaaatt actctttaaa gcttcaagtt catttaataa gttatccata
    19081 gttatacctc caagataaat ttaataaaat tgtacatatt aattatataa aagtcaagga
    19141 gtacaaacta ttgtataatt agctgtattg ttttgagtgt aaaagatttg aagcaatatc
    19201 tggatagtta gtaaaaatac catctacatt gagttttagg cagtgtagta tatcatcctc
    19261 ttcatttaca gtatatacat tgacaccaag tagatttttg tgacaaagtt caattagaga
    19321 ttcatttact aaaactagag gtggatgaag ataactatta cccagaaatt tagcaaaatt
    19381 gactacattg tttataggta aataacataa taatcctgtt tttatactag ggtctaattt
    19441 atgaaagttt tttaaactat tgctgttgaa actagatata agaactctat ttttcatatt
    19501 atactttaaa ataagctcta aaacgtcttt ttctatattg ggatagttta ttttgtcagt
    19561 ttttagttct atatttaaaa taaggtctgt aggtgaaatt agaattaata cttcttctaa
    19621 agttggaatt ttacattctt tattgctttg atatccttca aaagatgagt ttagagtttt
    19681 taattttctt aaagtaaagt cttttacaaa accatgtcca ttaaaagttc tatcaacctt
    19741 ttcatcatga ataactacaa tttttccatc cttagttctg tggacatcaa gttctattcc
    19801 atatatatcc atatccaaac attttttaaa agctagaata gtattttctg gatactttcc
    19861 actaaacccc ctatgagcat aaatattcat ttatatggtt ccctcctata agtataactt
    19921 atcttataaa aatttaatta atataatata tcatattcca aaaggtattg ttatatatta
    19981 gttaaataac tatttgtaat ttatatatta tataaatgta gaaaaataaa atcatatatg
    20041 tgttataata tattttaaac attgtaaata ttacgaattt agtagtcata ataagtgtaa
    20101 ttttatgcaa gctcatgttt ttagagcttg catatgtatt attaatatat ttatatattg
    20161 tgaaaataaa attccaaaag aattttttct atagacttag tagtaagggg gaattttgat
    20221 atgtatgaag ttaatttaga acgagctatg aattattgga tggatttgtt agatgattat
    20281 acgaatatag caaaaattcc aaatgatttt ttttataaag attgttctga tgaagtagta
    20341 gatgaggttt ttaagatgaa tattgagaaa catgaaaggt taattcaata ttctaaagtg
    20401 aacaatatta gcatagactc tatcatagaa actatatatg gccttatact tcaaaagtat
    20461 atatatgaag atgatgtagt ttttggtaaa acaatgaata taattccaat aagagtaaga
    20521 gttagacaaa gtgaaaaatt tatagatgtt gtaaggaagc taaaaaggca gtggagcagg
    20581 tctttagagt atggatacta tttgttatca gatatagaga ataaaaatca tctaaatact
    20641 aagcttataa acacaatatt tatagctaaa agttttgatt tatcatatga ttatgacata
    20701 gaaaaaa
//
LOCUS       DAEMHZ010000059        20690 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000059 DAEMHZ010000000
VERSION     DAEMHZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.059"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1047
                     /locus_tag="KPK72_002096"
     CDS             <1..1047
                     /locus_tag="KPK72_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2489312.1"
                     /translation="TFGCCRFVYNKYLAKRIDVYKSDKETFTYKQCSSDLTNLKKELK
                     WLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINRFSYKTNFTNGNIMYCGQ
                     YIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLCCTDVDIEVFENTNNHIGL
                     DLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVAGLQE
                     HIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEY
                     KANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASIN
                     ILKEGLRLITIQNK"
     gene            1165..1470
                     /locus_tag="KPK72_002097"
                     /pseudo
     CDS             1165..1470
                     /locus_tag="KPK72_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1483..2388
                     /gene="truB"
                     /locus_tag="KPK72_002098"
     CDS             1483..2388
                     /gene="truB"
                     /locus_tag="KPK72_002098"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG2489313.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2519..3448
                     /locus_tag="KPK72_002099"
     CDS             2519..3448
                     /locus_tag="KPK72_002099"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG2489314.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3552..3809
                     /gene="rpsO"
                     /locus_tag="KPK72_002100"
     CDS             3552..3809
                     /gene="rpsO"
                     /locus_tag="KPK72_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG2489315.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            4049..4894
                     /locus_tag="KPK72_002101"
     CDS             4049..4894
                     /locus_tag="KPK72_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2489316.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            5019..7130
                     /gene="pnp"
                     /locus_tag="KPK72_002102"
     CDS             5019..7130
                     /gene="pnp"
                     /locus_tag="KPK72_002102"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG2489317.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            7396..8139
                     /locus_tag="KPK72_002103"
     CDS             7396..8139
                     /locus_tag="KPK72_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2489318.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            8297..9544
                     /locus_tag="KPK72_002104"
     CDS             8297..9544
                     /locus_tag="KPK72_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2489319.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVANNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9579..9824
                     /locus_tag="KPK72_002105"
     CDS             9579..9824
                     /locus_tag="KPK72_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2489320.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9834..11057
                     /gene="dapG"
                     /locus_tag="KPK72_002106"
     CDS             9834..11057
                     /gene="dapG"
                     /locus_tag="KPK72_002106"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2489321.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            11170..11904
                     /locus_tag="KPK72_002107"
     CDS             11170..11904
                     /locus_tag="KPK72_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG2489322.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            12027..14438
                     /locus_tag="KPK72_002108"
     CDS             12027..14438
                     /locus_tag="KPK72_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG2489323.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            14441..15499
                     /gene="mnmH"
                     /locus_tag="KPK72_002109"
     CDS             14441..15499
                     /gene="mnmH"
                     /locus_tag="KPK72_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG2489324.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15493..16827
                     /gene="rimO"
                     /locus_tag="KPK72_002110"
     CDS             15493..16827
                     /gene="rimO"
                     /locus_tag="KPK72_002110"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG2489325.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16814..17356
                     /gene="pgsA"
                     /locus_tag="KPK72_002111"
     CDS             16814..17356
                     /gene="pgsA"
                     /locus_tag="KPK72_002111"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG2489326.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17644..18690
                     /gene="recA"
                     /locus_tag="KPK72_002112"
     CDS             17644..18690
                     /gene="recA"
                     /locus_tag="KPK72_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG2489327.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18866..20407
                     /gene="rny"
                     /locus_tag="KPK72_002113"
     CDS             18866..20407
                     /gene="rny"
                     /locus_tag="KPK72_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG2489328.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8479 a         2101 c         3977 g         6133 t
ORIGIN      
        1 acttttggat gttgtagatt tgtatataat aaatatcttg ctaaaagaat agatgtttat
       61 aaaagcgata aagaaacttt tacttataaa cagtgtagtt ctgatttgac caatttaaaa
      121 aaagaattaa aatggcttaa ggaacctgac aaattttcac tacaaaacgc cttaaaagat
      181 ttagataatg catataaaaa attctttaaa gaaaaggcag gatttcctaa atttaaatca
      241 aagaaaatta atagattttc atataaaact aactttacaa atggaaacat tatgtattgt
      301 ggtcaatata taaaactacc taaacttggt atggttaaga taagggataa acaagtacct
      361 caaggtagaa tacttaatgc tactatatct aaagaaccaa gtggtagata ttatgtatca
      421 ttatgctgta ctgatgtaga tattgaagta tttgaaaata ctaataatca tataggttta
      481 gatttaggaa ttaaagaatt ttgtatctca agttgtggag actttataga aaatcctaag
      541 tatcttaaga agtcattaaa caagcttgct aaattacaaa gagaattatc gagaaaaaca
      601 atcggtagtt taaatagaaa taaagcaaga ttaaaagttg caggacttca agaacatata
      661 gctaatcaaa gaaatgattt tctacaaaaa ctatctacta aattaataaa agagaatgat
      721 attatttgca tagaagattt acaagtaaag aatatgatta gaaatcgtaa actatctcgc
      781 ttaatttcag atgtatcatg gtcagaattt attcgtcaat tagaatacaa ggctaattgg
      841 tatggtagaa aaattgtaaa ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa
      901 tgtgggtata aaaacgagga agttaaggat ttaaatataa gagaatggat ttgtcctagt
      961 tgtaatgaaa ctcacgatag agatataaat gcaagtataa atatattaaa agaaggatta
     1021 agactaataa cgattcaaaa taaataactt atgtaagaac cgtaggaact acggggatag
     1081 cctgtggaga attagtaaga catattttaa tatgcaaaat tctgtgaagc aggaaccctg
     1141 tgactttagt catgggaggt tcagtcaaag gtatctagta tagttttaac tcaagacatg
     1201 atgaatagaa ataatattag ctttaatgat gttgatggaa taacaacata tacaagagac
     1261 ataaaaggta tagaagttgg aattttattt aaagagaaaa aacaaaatga aattaaagtg
     1321 agttttaggt caaaaaatta tgttgatgta agtgaaatag ccaagttatt tggcggtggt
     1381 ggtcatgtaa gggctgctgg atgtactatt agagatagta ttgataatgc aaagaaaatg
     1441 gtactagagg cagtgttaaa atcaatttaa ggtgacagta aaatgaataa gattataagt
     1501 atattgaaac ccacaggtat gacatctcat gatgttgtaa gtagagtgag aaagatttta
     1561 aatataaaaa aagttggtca tacaggtact cttgacccag atgcttcagg tgttttacca
     1621 atctgtatag gaaaagctac aaaagtatgt gaagttattt taaacaaaga taaatcgtat
     1681 atatgtgagc ttactttagg tataagtaca gatacttatg atgcttcagg tgaaatttta
     1741 aaaaaagttg atgattttaa atttagtaat gaagacatag aaagagcttt tgatacacaa
     1801 agaggagaga taaatcaact tcctccaatt tattcagctt taaaggtaaa tggaaagaga
     1861 atgtgtgatt tagtaagaag tggtagacaa tctgaaatta ctttaaaaac aagaagagta
     1921 aatataaaag acataaaaat actaagtata aaaggtaata aagtaatgtt ttatgttgag
     1981 tgttctaaag ggacttatgt gagaagtatt tgccatgata taggagaata tctaggttgt
     2041 ggagctcata tgtcgttttt gaatagaaca tcttctggta aatttgactt agataattca
     2101 ataacattag aagaattaga attattttat gaaaataaaa ctttagacaa atacttatat
     2161 gatatagatt atgtccttga ttcgtttaat tatgttgtat taaaccctaa tgctataaaa
     2221 tattatagta atggtggctc aatagatgat aaaaggtttt taaaaaataa tttcgataaa
     2281 gatgatgaat ttgtaagagt atacagtaca gataatttct taggattagg aaagttatca
     2341 aaacacaata atacaataag tgttaaaagt gataagatgt tcatataacc aatgtattta
     2401 aaaaaacaac aaaagagata gtactataat aacttctata gtctatctct tttacttatg
     2461 ctataatagt ttgtgaataa gttaagagta aattatattt tagcggggaa catttattat
     2521 ggtgattata aaatctataa aagaaatagc aaacataaaa gaaagtgtca ttaccatagg
     2581 aaattttgat ggtgtacaca aaggacatca agttcttata ggaaaaacag ttgaacatgc
     2641 aaaaaaagaa aatatacaga gtattgtatt tacatttgca aatcatcctg taaattactt
     2701 tagaccaaac tcaacaaaaa atattataag caatgatgat aaaataaaat tattagaaga
     2761 tttgggaata gatatagttg taatcatacc atttgatgag tatatgacta aaatacctgc
     2821 aaaggatttc gtagatgaga tactagtaaa aaaattaaaa gctaaaaaga tagtaattgg
     2881 acatgacttt acatttgcaa gggcaaaaga aggcaatgtt aatctattaa aaagcttaga
     2941 acatgaattt ggttttgagg tagaagtaat aaaacccatc aaaatcaatg atgtaagagt
     3001 gagtagtact tatattagaa gtttagtatc ccagggtgat atggctaatg tcaaggaata
     3061 tttgggtaga aattacaagt tagaaggttg tgttatacat tctaaacacc ttggaagaac
     3121 tataggattc ccaacggcaa atattgacct taagaataat atgctcgttc caaagagagg
     3181 tatttatgct tctattgtac atattggaga tgagacttat tttggtgcta caaatgtagg
     3241 ctataaccca acagttaatg gaaaaacttt gtctattgaa acaaatattt tagagtttga
     3301 tagagatatt tatggggaaa atataattgt tgaatttttg gaaagaataa gagatgaaag
     3361 aaagtttaat tcaatagatg acttaaaaaa tcaattgtat aatgatacaa attatgtgta
     3421 tgaaaactat gtttgcaaaa acaagtaatg tatgataaaa tgattaagga aaataaccat
     3481 tactcggcat ttgaatactc caatcaatgc ttagtaatag gcgtttctaa aaaaatacgg
     3541 aggtaatcta aatgataaac aaatcagaaa taataaaaga atatggaaga gctgaagggg
     3601 atactggttc tccagaagta caaatagctt tattaacagc tagaataaac gagttaaatg
     3661 gacatttaaa aactcataag aaagaccacc attcaagaag aggactatta aaaatggttg
     3721 gtagaagaag aaacttatta gcttacttaa aggaaaaaga tcttgaagga tatagagcat
     3781 taatagctaa attaggatta agaaagtaat ttcattcttt ataaaaaagc aggtatagtg
     3841 tatacctgct tttttatagt taattcatta taaagtaaaa tagttaataa taaaagtagt
     3901 ttatatgtta taaaaaatta taaaatgaat aatattgggt aagaatatta aaatagtata
     3961 caaatacata atcaatttaa aaggaaagac aggataaata tagaaatttt ataacaggat
     4021 aattagttgg aataaggagg atattatttt gaaaataaag ttagtatgcg atagtttgtg
     4081 cgatattcca gatgaaatat cggaaaaaga ttatatagag atagttccac ttacagttat
     4141 ttttaatgat agggaatata tagaaggagt agatataaaa aaagaagagt tttataaaaa
     4201 agtaaaagaa atcaagcaaa tacctaaaac ttcacaagct acatatatgg agtttaaaga
     4261 agtgtttgat aagttcataa cagaaggtta tcatataatc tgtatgacag gggctgcaaa
     4321 cgcatcagga acttttcaaa gtgctatgat agctaaaaat gatgtaaatg aaaatgaaaa
     4381 aatacatata tttgatacta gaaatttatc tctaggtagt ggacaatatg ttataaaggc
     4441 atgtgaactt cttgaagaag gactgggatt tgaagaaatt atagatgaat tagaaaatac
     4501 tcgcagtagt gtaagattgc tttttgcacc atatacactt gattttttaa agcagagtgg
     4561 aagagttcca gttgcaactg ctttgatagg aaatatgtta aatataaagc ctatattttt
     4621 ctttgataat ggggaagcta agttggtaaa taaggttaga ggaataaaga atatagcaag
     4681 taaattggtc gatataattt tagaaatgaa tgaagggtct ttagaaggaa aaatcgtcac
     4741 aataggttgt ggagataatt tacacgattg tgaaatatta aaagatgaag taaataaaag
     4801 aataaaagca agaagagtat tatttactcg aggaggagtt tctatttgct ctcatacagg
     4861 gccagatata ttggctataa gttgttctaa ttagtagtaa tataaggttt ttgcatatat
     4921 atgtgaagat aattttatat tgttaattta tagtattttt gataatatag taaatagagt
     4981 gatatacgtg ttttttaata agaggaggac aagcattaat gtttgaacat aaaatattta
     5041 aaatggattt tgcaggaaga gaactttctg tagaaatagg aaagatttgt gaaatggcta
     5101 gtggtagttg tatagttcga tacagtgata gtatggtaat ggtaaataca actaaatcag
     5161 ccaaacctag agacggaata gacttttttc ctttaagtgt ggattatgaa gaaaaattat
     5221 actctgtagg taagatacct ggaggttttt taaagagaga aggcaagcct tcagagaaag
     5281 ctatacttac atcaagattg atagatagac caataagacc tttattccca aaaggattta
     5341 gaaatgatgt tcaggtagtt gcaacagttc tatcagtaga ccaagattgt acaccagata
     5401 tagtagcaat gataggttct tcaatagccc tttctatatc agacatacca tttaatggac
     5461 caacaggttc agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat ccaaacgcag
     5521 agcaaagaga aaaaagttca atgcatctag ttgtttcagg tacaaaggaa gcaataatga
     5581 tggttgaagc cggagctgat gaagttccag acgaggtaat gcttgatgct attttatttg
     5641 ctcaccaaga aataaaaaaa atagttgagt ttatagaagg tatagttgca gaagttggaa
     5701 aagaaaaaat gcctgttgaa ttataccatg caggagaaga aataactcaa ttggttcgcg
     5761 aatttgcaac tgataagatg aaaaaagcag ttcagacttt tgaaaagtta gagagaatgg
     5821 aaaatatgga tagagttaaa gaagaaactt tagctcactt tgaagaaact ttagaagatt
     5881 ttgaagattt tgttggagat atagaagaag tattacaaga tataataaaa gaagaagtaa
     5941 gaaaacttat agtacatgaa aatgtaagac cagataatag aaaacttgaa gaaataagac
     6001 ctatatggtg tgaaactggc atgataccta gagctcatgg ttcagccata tttactagag
     6061 gacaaactca agtacttaat gttgctactt taggtgcttt aggagatgtt caaaaactag
     6121 atgggttaga tgaagaagaa aacaagagat atatgcatca ttataatttc cctgcatata
     6181 gtgttggaga agctagacca tcaagaggac caggaagaag agaaatagga catggtgctt
     6241 tagcagaaag agctttatta ccagtaatac catctcaaga agaatttcct tatgctatta
     6301 gattagtttc tgaagtatta agttcaaatg gttctacatc tcaagcaagt gtatgtggct
     6361 caacattatc tttacttgat gcaggtgtac ctataaaaga tatggttgca ggtatagcaa
     6421 tgggacttat aaagcatgat ggtaaggttg cagtgctttc agatatacaa ggtatggaag
     6481 accatttagg cgatatggac tttaaagtag ctggaacaga gtatggtata acagcaatac
     6541 aaatggatat aaaaattgat ggtatagata aagaaattct acaacgtgct ttaaaacaag
     6601 ctaaagaggg tagaatccat atactaggag agatgagaaa aactatatct cagccaaaac
     6661 cagaactttc accatatgca cctaagattg ttaaaatgca aataaatcct gacaagataa
     6721 aagatgtaat aggacctggt ggaaagataa taactaagat aatagacgaa actggagtta
     6781 agatagatat tgaacaaact ggggaagtat ttatatcagg tattgagatt gatatgatta
     6841 agaaagctca agaacttata aataacatag tagttgaacc agaagtagga aaaacttata
     6901 aaggtaaggt aagtagaata atgaactttg gagcatttgt tgagatactt ccaggaaaag
     6961 aaggattact tcatatatct catatagctc atgaaagagt tgccaaagtt gaagatgtat
     7021 tgaatatagg ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa ggaagagtta
     7081 atttatcaag aaaagttctt ttaccaaaac cagaacataa aaataaataa cttattatat
     7141 aattaaaagc aaagtcttag gattttgctt ttttaaatac aaaaaagtag ataattttaa
     7201 aatgatagtt atggatggct tatagttctt tttctgtaag gctttactct tataaagaag
     7261 ctatttaaaa gctgattaat agaaagcttt attgatatat atagaatcac ttaagttata
     7321 tttagtgcat aaacagagct tatttttaat agtattaaat aagaaacaat ttgtacataa
     7381 ttagggggag ttttaatgat aatgatggta cttaataaga gtaagctcaa aaaaatcata
     7441 atactaattt tagtagtgtt attagtatta tcaggagggg taatggtatt aaagttattt
     7501 aacaacaagc cagtatttaa tttatttaat ggtgtagatt taccatatga aagaggtaca
     7561 aaggataaaa gtggatatgt tgcattaacc tgtaatatag accttggatg ggaaacagag
     7621 tacgtagaat ccattttaga aacacttaaa aaagaaaatg taaagattac atttaatgta
     7681 actggaaaat gggcagaaaa gaataaggat gaattattaa agataaaaaa acaaggacat
     7741 gaaataggta atcatggata caaacattta gattattcta cattatcata tgaagacaat
     7801 tatgagcaaa tagagacctc taaaaagata attgaagaaa ttataggtga aaaaactaaa
     7861 ttttttcaag caccagcagg ttcttttggt ccagaaacag taaaggctgc taaagcatta
     7921 gggtatactt ctatcaaatg ggatgccgat actatagatt ggaaatacaa agaccaacca
     7981 gaggtaatca ttgatagaat gaagaaaaaa gacattaaag atagtagtat aattttaatg
     8041 catccaacca acgcaacgac aaaatgtata gatgatatta tagctattgt aagagaaaaa
     8101 ggactaaaac caggaaaact tagtgatgta tttaagtaga caaagtataa atatttggtt
     8161 aagattgtat aagttgtagt tgaattaatt aaaagactga cttataatgt aatacatata
     8221 aaaaataaat ataattaaaa catagtgttt tcaataatcc aatatgaaac aaagaataaa
     8281 ttgttaggag aatgtaatgt ataagacaaa aatactggaa aatgggctta caataattgg
     8341 tgaagaaata ccatatctaa agtcaataac attaggtatt tggataaatg caggctctag
     8401 gatagaagaa gcacaggtga gtggaacttc tcattttata gagcatatga tgtttaaagg
     8461 aaccaaaaac agaacatcaa aagagattgc tagttcaata gataatttgg gtggtcaaat
     8521 aaatgcattt acaagtaaag aatgtacatg ttattatgta aaactaatag atgagcatat
     8581 agatacagga attgatgtat tgagtgatat gatactaaat tctaaatttg ataaaaatga
     8641 tatagataag gaaagactta taatattgga agaacttaag atgtatgaag actctccaga
     8701 tgacttatct tatgatttat tagtggaaaa tatatatgct aatgatggat taggaatgaa
     8761 tataattggg acaaaagaat ctctatacaa tataactaga gaatccatgt tagaatactt
     8821 aaataagtat tatattccaa ataacgctgt tatttctata gctggaaact ttaactttga
     8881 tgatatggtg gagaaaatca aatcaaaatt tggacactgg gaaaagaaaa atttgagtat
     8941 agacataagt gaggctaagt ttaatccttg ctttatatca aaaaataaag atactgaaca
     9001 ggtgaactta gctatgtgcc taaaaggtat tccttttgaa aatgatgaag aagtctattc
     9061 aatggcagtg gcaaataata ttttcggtgg aagtataagc tcaaggcttt ttcagaaaat
     9121 aagggaagaa aaaggtttag tatattcaat atactcatca cagacattat ataggaaatg
     9181 tggtgaatta ggtatatttg caagcatgag tacagaaaat ttacaagatg tatataattt
     9241 aataaagaaa gaaattgaaa atataagaga aaactatctt acagaaaaag aaatatctga
     9301 aagcaaggaa cagttaaaag gaaattatat attggattta gaaagtacta gcagtagaat
     9361 gatgtctact ggtaaatcta tgttacttag caaaaaagta aaaactacag atgaaatact
     9421 tgaatgtata aataatgtta atattaattc aataaagaag gttgttgata aggtattcaa
     9481 tatcgaaaat attggtacat gtatagttgg aagagatgta gaaaagattc tacacttgga
     9541 ttaatattga aaaaagtaaa tctctggagg tataaaatat gaatttatct gaaatagcag
     9601 gtaaggaaat agttaacttg gtcacaggag aaagattggg ggtcattgga gaatgtgacc
     9661 ttatactaga tgaaactaca ggcggtatat tagcgcttct aattccaaaa gaaaaaggat
     9721 tttttgccag aagaaaagat aagtcatttt tagaagtacc atggagaaat gtaaaaaaaa
     9781 taggtaatga catgataatt atagaatatg agggatatat ttaggaggaa aaaatgagta
     9841 ttttagtgca aaagtttggt gggacatcag tagaatcata tgaaaaaatg aatgaagtat
     9901 gtaagatagt aaaagcatac aaaaaaaatg atgaagaatt acaattagta ttagtagttt
     9961 cagcaatggg aagaaaagga gctccatatg ctacagatac acttataaat ttgtgtagtg
    10021 cagtaaatga tgagccaagt aaaagagaac ttgatttaat aatgtcttgt ggagaaataa
    10081 tatcaggaac aattcttgct aatttattga atgcacaggg gatagattct gttttcttta
    10141 ctggtcagca agcaggtata attacaagtg atgaatattc taatgcaaag ataaagtata
    10201 taaatcctaa aaaggtaaaa agagctttag atgatggtaa ggttgtagtt atagctgggt
    10261 tccagggtgt aacagatgat ggagaaataa ctactcttgg aagaggcgga agcgatacat
    10321 cagcagttgc tattggtaaa gctttagaat gtgaaacagt tgaaatttac actgatgtgg
    10381 atggtataat gactgcagac cctagagtag aaccaaacgc taaagtattg agttttatag
    10441 actatgagga agtattccaa atggctgaca aaggtgcaaa agtaatacat ccaagagcag
    10501 tacagttggc taaaagtgga aatataactt tagccataaa gaatacaatg aatccaactt
    10561 ttgaaggtac aaaaataggt tctttatgta gacacttaga agacaatata gaatatgaac
    10621 aagaaagaga ttttaaggtt gctgttgcaa ataaagatag cgtagcacaa gttaagatta
    10681 agtcagcaga agaagtgttt acagaagtat taaatgagat agaaaagaaa cttataacta
    10741 tagatatgat aaatttcttt atatcagaaa aagcatttgt tgtagaagat gctgatataa
    10801 aatcattaaa ggaaatacta gataaatttg aattagatta tgaagtaaat agagactgtg
    10861 ctaaggtaac attgatatgt tcaagaattg ctgatgaaat gtctggtatc atgtcaaagg
    10921 tagttagagg attatcaaaa gcaggggtat ctcttttaca aacatcagac tcaaatatga
    10981 ctatatcttg tttagttagt gaagaagata tgcatacagc agtacatgct atacaccaac
    11041 aattttattt aaaataaact gtataataca attatttttt aatcaaattt aagaatttca
    11101 tatatatctt aatatatttg atttatttgg taaaactaaa ttaaatataa ttttgatagg
    11161 agttttttta tgaaagatta tagagaagat aatgaaagat ttgataaaga taattgtagt
    11221 gaagaatcta gttgcgaaga gagagaatgt ataaagcaat ttggtaccaa tgaaatgcca
    11281 cctcaacctc ctaaagatat tcaatgtata actataattg gagaaattga gggacatttt
    11341 ataggaaatc ctcaaaaaaa agctacaaag tacgagcata taattccaat gctatactca
    11401 attgaggaaa gtaatgatgt taaaggagtg ttagttgttt taaacactgt tggaggagat
    11461 attgaagcag gtttagctat tgcagaactt ttaaacagta catctaaaaa agtagttact
    11521 cttgttctag gaggaagtca tagtataggt gttcctcttg ctacagcagg agattactca
    11581 tttatagcac caacagcaac catgattata catccagtgc gaactactgg tcttgtgatt
    11641 ggtataaatg agacttttga atattttaaa aagatgcaag atagaataat tcaatttata
    11701 ataagaacat caaatatcaa aaaagatgta cttgaaaaac ttatgcatga aaaagatgaa
    11761 cttgtaagtg atgttggtag tgtattaata ggaaaagaag ctgtggatta tggtcttata
    11821 gatgaagttg gaggactaaa agaagcactt aaaaaattaa gagagcttat aaaagaatct
    11881 gaggaagaaa aaaataatga ttaaaatttg tttaaataat aaaatttatg atataatgat
    11941 gtaataaagt aggagtgtta gtgttaaggc attagcactt tttgctgtga aggggtaaaa
    12001 cttgctaaaa ataaggagtg atattaatgg ccacgaaaaa gaagaaaaaa aagaaggtaa
    12061 ataccttaag ttttaatgca gaatatcata atttaataac aatatttata ggtgtatttt
    12121 tattatatag tttaaattct aattctatgg gatggatacc tgtgttaatg caaaatcttt
    12181 ttaagggact atttggaggg ctttcaatag caataccatt tatagtaatt ataacaggac
    12241 ttttaggatt ttttgatgga aatgaatata tatataggct aagaaagact aaactatact
    12301 acataattat tttatttata tttgtatttt atggattatt aaatgcagga actcttcctg
    12361 tagatagccc actaaaaggt aacatgtttg atgatgttat gaaattgggt gtaagtggac
    12421 aaggtagtgg tttgatagca acaactattg cgtattatat gagtaaaata tttggtatag
    12481 caggtggatg gttaataagt atcttcgcac ttatactttc tgtaatgttt atatttaata
    12541 tttctataaa agatttggtg tcaaatgcaa aatcaaaatc taaagcatct aaagacagca
    12601 atttaacgtt taaagataaa attgcaaata tgaaaaaatc tgcaatagat atgatgactg
    12661 atgaagtaga tgatactact ataaataaaa aaccaggttt ctttaaaggg ttaatgtcaa
    12721 aaggtcttaa taatgatgat gaagaagatg aatattttga agaagaaaat agtgatggtg
    12781 tagatgataa aactataaaa atagttggat ttaataaggc agaagatgag tatttagaaa
    12841 tattagaagg aactcaaagt atgccagagc tagatgtttt aaaagaatta caaaaagcta
    12901 ccaatgaaaa tccagttgta gatactaaac cagaaaaaaa agttgatata gctaaaccta
    12961 atttaaatat agaaaaaact caacctatga gtattgtagc agagccagtt aatgaggatt
    13021 attcaaacta taaaaagcct agcattgagc ttttaaataa agtaaataag aagtctgatg
    13081 aaaatggtaa aaagaaagtg ttgaaaaatg cttctctttt agaaaagaca ctatcagatt
    13141 ttggagtgga agcaaaaatt aatcaagtta cagttggacc tactataaca agatatgaaa
    13201 tacaaccaag tccaggagtc aaggtaagta aaattgtaaa tttaactgat gatatagctt
    13261 taagtttagc agctaaaagt ataagaattg aagccccaat tcctggtaaa agtgcaattg
    13321 gaatagaagt tccaaatgaa gaagctcaaa tggttggtgt gagagaagtt ctagaaagtg
    13381 aagaatttaa taattttaac tctcctcttg caatgggact tggaaaagac gtagcaggaa
    13441 aaattataat tggagatata ggtaagatgc cacacttact aatagctggt tctacaggtt
    13501 caggaaagag tgtgtgtgta aatacattaa taagtagtat tttatataag gcaaatcctg
    13561 atgaagtaaa attactatta atagacccaa aagtagttga acttgcaaat tataatggaa
    13621 taccacatct attgatacca gtagttactg accctaaaaa agctgcaaat gctttaaatt
    13681 gggcagttac agagatgaat agaaggtaca aattatttgc agatgctcaa gtaaaagatg
    13741 taacaagtta taatgagaaa gcggaagaaa aacttcctaa aatagtaata ataatagatg
    13801 agttagcaga tttaatgatg gcaagtgcaa atgatgtaga agattatatt tgtagattag
    13861 ctcaaatggc aagagctgct ggaatgcact tgattgtagc aactcaaaga ccatctgttg
    13921 acgttataac aggggtaata aaggctaata taccatctag aatagcattt gcagtatcat
    13981 ctcaaactga ttcaagaaca atccttgata tgggtggtgc agagaagctt ctaggtaaag
    14041 gtgatatgtt attctatcca cttggtgcag caaaaccagt tagacttcaa ggggcattca
    14101 tatcagagag tgagtcggaa aaagtaatcg attttgtaaa aagtcaggtt aaagatggaa
    14161 taaaatatga agaagatata attgaaacta tatctaaggt taatacttca aagggtagtg
    14221 atgaggatga gtttttgagt gaagcgatag aatttgtcgt ggaaagtggt caagcatctg
    14281 cctctatgct acagagaaga tttaaaatag gattcaatag agcagctaga cttatagatt
    14341 ctatggaaga aagaggaata attggagcaa gtgagggaag taaaccgaga aaggtattaa
    14401 taagcaaaca ggatttgcag aatttagaag gtgaatagaa ttgtcagtta taaaaataga
    14461 aaaagcactt aagctagata aagtaatatt tgtagatgtg agaactgaag gagaatatga
    14521 agaagaccat atattgaatg caatcaatat gcctcttttt aaaaataatg aacataatga
    14581 agttggaaca atttataaga tgcagggaaa acatgaagca atacaaaaag ggtttgacta
    14641 tgtatcgtat aaattaaagg atatatattt acaagcagca gaacttgcat taaattatga
    14701 taatattgtc atatattgtg caagaggcgg aatgagaagt ggcagtatag taaatttatt
    14761 atcatcatta ggtgtaaatg tatatcaact tgaaggtgga tataaggctt atagaaattt
    14821 tgttttagag tatttaaggc atgtaatgga tacaaaaaac tttgtatctg ttcatggatt
    14881 gactggtaca ggaaagactg atttgttaca tttattagat gaaaaaggaa tagacgtact
    14941 ggatttagaa ggcatagcta aaaacagtgg ttctaccttt ggttttataa cttttgataa
    15001 aaagccacca tctcaaaaga actttgaaac aaaactattt gaaagtatat ttttttcgaa
    15061 agagaattat atatttgtcg agagtgaaag caaacgagtt ggaagtgtta cagttccaaa
    15121 tgaaattttc gaggcaatga cacgagaagg acatcatata ttattagaat gtgatattga
    15181 aaatagagtt gatagacttt gtagagatta tatttatgat aaagatgaag gtaatatatt
    15241 gattttaaaa gaatgtataa acaagtttag aaaaagattg ggtaataaag tagtagatga
    15301 ttatattgat ttattagaat caggtgaata taaagaactt gtaaaaagat atcttttaga
    15361 atactacgac ccactttata tgcattcagt agagaaatat aagtatagta agattataaa
    15421 ttttaatgat acacaaaaag ctttagaaga tgttatagac ttatatgaga gtatattgga
    15481 aggagaaagt gaatgttaaa aatagcattg gaatcattag gatgctcaaa aaatttagta
    15541 gatgcagaaa taatgatggg aattttaaat aacaaaggct ataaattaat tggagatttt
    15601 gaagaagctg atgtaattat agtaaataca tgtgggttta tagagtcagc taaacaagaa
    15661 tctatagata cgataataaa ctttgcagag ttaaaaaaaa ctggcaattt aaagttgttg
    15721 atagtaacag ggtgtctagc ccaaagatat tctgaagaat taaaaactga aattcctgaa
    15781 atagatgcta tagtaggaac aggaagctat caaaatatag ataaaatact taaagaatta
    15841 agtgaaatgc atcaaattgt aagccttaat gatatagagt ttgtatttaa tgaagattta
    15901 ccaagatata tatctacacc tagctatatg gcctatttaa aaattggaga aggatgttcc
    15961 aataattgta cttattgtat aataccaaaa ctaagaggta aatatagaag tagaaaattt
    16021 gaagatataa ttaaagaagc taagaaatta gctgaatctg gagtaaaaga acttgtggtt
    16081 atagcacagg atacgacaaa gtatggtttt gatttatatg gaaaagaaag attatcagaa
    16141 cttttagaag aacttgcaaa aatagatgga tttaagtgga taagggttat gtattcatat
    16201 ccagaatcta taacagagga attgatacaa gttataaaga aatatgataa tatatgtagt
    16261 tactttgata tgccaataca gcatgcaagt aacaatattt taaaattaat gaatagaaaa
    16321 actactaaag aagatatatt aaataaaatc aatctgataa gaagtaatat accagatgct
    16381 atacttagaa ctacaataat agtagggttt cctggtgaaa cagaagatga cttcaaacaa
    16441 cttgtggact ttgttgaaga ggttaaattt gatagacttg gtgcatttgc atattcaaga
    16501 gaagaagata caccagcaga tagattgcca aaccatatag atgaagaagt aaaaatacaa
    16561 agaagagata ctttaatgat gattcaacaa aaaatatctg aagaacttaa tgacaaaaag
    16621 ataggaaaaa cttatgaggt gttaatagaa gaacagatag aagataatgt atatacagga
    16681 agaacacaag gtgatgcaga agaaattgac agtatagtgt atgtaaaaag tgtagacaat
    16741 cttgaagtag gagaatttgt aagtgttcaa ataaatgatg caatggaata cgatttaatg
    16801 ggagatgttt tatatgaact tgcctaacaa gttaactttg tttagaatat ttttaatacc
    16861 agtatttgtt ttaattatgt tgctcaatgt accaaataaa tttttaatag cttgtattat
    16921 ttttataata gcttcaatta ctgatgcttt agatggaaaa atagctagaa aatacaatct
    16981 agtcactgac tttggaaaat ttatggaccc attagcagat aagttattgg ttatttcagc
    17041 acttacatgt atgatagaag accatcttgt aagtagttgg atggtaatta taatagtggc
    17101 tagagagctt acagttagca tattaagagc tatagctgct gctgatggaa aagtaattgc
    17161 tgctggaaac agtggaaaac ttaaaacgat aactcaaatg gtatctatag tgtttttatt
    17221 gttaggtgct cagtttgaaa atgtattgat attaaatatt ggtgaaattc tcattcttat
    17281 agctacacta cttacattat attctggatg ggaatatcta tataaaaata aagagctttt
    17341 tatgtcttct aagtaaaaaa ttatgcttta ataaataaaa actaatgatt aataaataaa
    17401 aaataacaac ttaaaatttt aaaaatgtaa aaaagggtat actattatag tatacctctt
    17461 ttcattttaa gaatattttc ttttgtgtat ataattatta ataatggttt ataaataaaa
    17521 tgtaatgaat aaaaatataa tcattagtag cgtttttaaa ttgactaggt ttgatttatg
    17581 ttatataatt aatatataac aagagaacaa atgtttgtag ataaatgaag ggatggtata
    17641 gagatgagtg tagatcaaga aaaattaaaa gcgttgaatg aagctttagg taaaattgaa
    17701 aaagattttg gtaaaggttc agtaatgaaa ttgggagaag caacatctat gtctatagat
    17761 gttatatcaa caggagcgat tggtttagac atagctattg gtataggagg tctacctaga
    17821 gggagaatag ttgaagtata tggtccagaa tcttctggta agactactgt tgcgcttagt
    17881 tgtgtagcat cagctcaaaa agatggagga atagctgcat ttatagatgc agaacatgca
    17941 cttgacccag tatatgcaaa agctttgggt gtggatgttg ataacctaat aatatctcaa
    18001 ccagatacag gtgaacaggc tttagagata gcagaggcat tgataagaag tggagcgata
    18061 gatataatag taatagactc agtagcagca ttagttccaa aggctgaaat agatggagat
    18121 atgggtgatt ctcatgtagg tttacaagct agacttatgt cacaagcact tagaaagtta
    18181 actggttcaa ttaaaaaatc aaattgtgtt gctatattta taaaccagtt aagagagaaa
    18241 gtaggaataa tgtttggaaa cccagaaact actactggag gacgtgcact aaaattctat
    18301 tcatcagtta gattggatgt tagaaaaata gatacaataa aacaaggtga taaagttata
    18361 ggtagtagaa ctagagttaa agttgttaaa aacaaagtag caccaccatt taagcaagct
    18421 gaatttgata taatgtatgg agaagggatt tcaaaaattg gagacctttt agatatagct
    18481 gctgatgtag atatagttaa aaaatcaggt tcatggtata gttacaatga tactaaactt
    18541 ggacaaggaa gagaaaatgt taaaaaattc ttggaagata atttagattt aactactgaa
    18601 atagatgaga aagttagagc attttacaat ttaaatgaag aacatgaaga atcaggtact
    18661 tcagtatcaa aagaaattgt agaagaataa gtaaaaatac aaaaaaataa ctagtaatta
    18721 aagacatgcc acgctcccta ttagcttgac acaatgttga aaaaattata aaattaagtg
    18781 aggataaaat atctaaattt aattttaatt attaggaata ttttttaatc atagtgatga
    18841 cattttggga ttattaacga aggaggtgga tgtcatagat agtgtagtaa tgattgtaat
    18901 aggtgcagct gttggtatta tagctggata ttttgttaga aaaaatatat ctgaagcaaa
    18961 aattggacaa gctgaaaact tggccaaaga aataatagat aaagcacacc acgactcaga
    19021 aacagtacaa aaagaaaaat tactagaagc taaagaagag atccataagt ggagaacaga
    19081 agctgaaaga gaaaacagag aaagaagaac agaagtacaa aagtatgaaa aaagagtagt
    19141 acaaaaagaa gaagtcttag atagaaaatt acaaaattta gaatctaaag aagtaaacct
    19201 aagtgaaaaa ttaaaggtag tggaaaagaa agaagaagaa gttgaagtaa taaaaactca
    19261 acagttagaa aaattagaaa gtatatcagg aattacttct gataaggcca aagaaatcat
    19321 cttaaccaat gcggagagag atgtaagacg tgaaatgtct ataatgataa aagagataga
    19381 aagtcaagct aaagaagaag ctgataagaa atcaagagaa attataggat atgcaattca
    19441 aaaatgtgca gcagatcacg tagccgaaac tactgtaaca gtagtaagct tgcctaatga
    19501 tgaaatgaaa ggtagaataa taggtaggga aggtagaaat ataaggactt tagaaacctt
    19561 aacaggtata gaccttataa tagatgacac tccagaagcc gtaattttat caggatttga
    19621 tccaataaga agagaaattg caagaattgc tctagaaaaa ttaatagcag atggaagaat
    19681 acatccagct agaattgaag aaatggttga aaaggcgaga aaagaagttg acaatattat
    19741 aaaagaatat ggtgaacaag ctgcatttga aactagtgta catgggattc atccggaatt
    19801 aataagatta ttaggtagac ttaactatag aactagttat ggacaaaatg tacttaaaca
    19861 ttctatagaa gttgctcata tagctggtat aatggctgct gaaataggag cagatattag
    19921 attggctaag agggcaggtt tattacatga tataggtaag gctgtagacc atgaaatgga
    19981 aggtacacat gttgaaatag gtatggattt acttagacgt tataaagagt ctaaagaagt
    20041 tatacatgcc atgagtactc atcatggaga ttacgagcct caaacagtag aggctgtatt
    20101 agttacagca gcagatgcaa tatcagctgc tagacctggt gcgagaagag aaactttaga
    20161 agcatatata aagagacttg aaaaattaga agaaatagct aattcatatg aaggtgtaga
    20221 aaaatcattt gctatacaag ctggtagaga aataagaata atggtaaaac cagaagctat
    20281 aaatgatgaa gaaatacatc tattggctag agatatgact aagaaaatag aggatgaact
    20341 tgaatatcct ggacaaataa aagtaagcat aataagagaa acaagagcta tagagtatgc
    20401 gaagtaaaag aaataatatc taaataataa gaaaatattt ttagaaattt aaatttgcca
    20461 caagatatga taatatatct tgtggctttt aaattgttta agtttaattt tatataatgc
    20521 aaaaataatc taagtgctaa aagtgctact tacaaaagat taataaaata aagctttata
    20581 tatgtattat ttatataatt atccaagttt gtttgatata atgaagttgt aaaatcaata
    20641 ctttactaca aaataaatag taaatactac agtaaaatct aaaaatagga
//
LOCUS       DAEMHZ010000060        20682 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000060 DAEMHZ010000000
VERSION     DAEMHZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.060"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            79..468
                     /locus_tag="KPK72_002114"
     CDS             79..468
                     /locus_tag="KPK72_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2489329.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEAMKLKYDWKKSTT
                     LTFLKTLVDKGFLTTYKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
     gene            792..1301
                     /gene="vanZ1"
                     /locus_tag="KPK72_002115"
     CDS             792..1301
                     /gene="vanZ1"
                     /locus_tag="KPK72_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG2489330.1"
                     /translation="MKSKKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1403..1597)
                     /locus_tag="KPK72_002116"
     CDS             complement(1403..1597)
                     /locus_tag="KPK72_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG2489331.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(2113..2505)
                     /locus_tag="KPK72_002117"
     CDS             complement(2113..2505)
                     /locus_tag="KPK72_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2489332.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            3072..3434
                     /locus_tag="KPK72_002118"
     CDS             3072..3434
                     /locus_tag="KPK72_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489333.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRVQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            3418..4125
                     /locus_tag="KPK72_002119"
     CDS             3418..4125
                     /locus_tag="KPK72_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489334.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKKLVQVYKYRGV"
     gene            4145..4564
                     /locus_tag="KPK72_002120"
     CDS             4145..4564
                     /locus_tag="KPK72_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489335.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            4554..5078
                     /locus_tag="KPK72_002121"
     CDS             4554..5078
                     /locus_tag="KPK72_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489336.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNISNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYANIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            5390..5947
                     /gene="efp"
                     /locus_tag="KPK72_002122"
     CDS             5390..5947
                     /gene="efp"
                     /locus_tag="KPK72_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG2489337.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            6039..6494
                     /locus_tag="KPK72_002123"
     CDS             6039..6494
                     /locus_tag="KPK72_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2489338.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            6657..7367
                     /locus_tag="KPK72_002124"
     CDS             6657..7367
                     /locus_tag="KPK72_002124"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG2489339.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            7421..8491
                     /locus_tag="KPK72_002125"
     CDS             7421..8491
                     /locus_tag="KPK72_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2489340.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            8519..12073
                     /gene="smc"
                     /locus_tag="KPK72_002126"
     CDS             8519..12073
                     /gene="smc"
                     /locus_tag="KPK72_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG2489341.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESVKCSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            12100..13380
                     /gene="ftsY"
                     /locus_tag="KPK72_002127"
     CDS             12100..13380
                     /gene="ftsY"
                     /locus_tag="KPK72_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG2489342.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            13483..13851
                     /locus_tag="KPK72_002128"
     CDS             13483..13851
                     /locus_tag="KPK72_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG2489343.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            13848..15200
                     /gene="ffh"
                     /locus_tag="KPK72_002129"
     CDS             13848..15200
                     /gene="ffh"
                     /locus_tag="KPK72_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG2489344.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            15238..15510
                     /gene="rpsP"
                     /locus_tag="KPK72_002130"
     CDS             15238..15510
                     /gene="rpsP"
                     /locus_tag="KPK72_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG2489345.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            15575..15802
                     /locus_tag="KPK72_002131"
     CDS             15575..15802
                     /locus_tag="KPK72_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG2489346.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            15910..16425
                     /gene="rimM"
                     /locus_tag="KPK72_002132"
     CDS             15910..16425
                     /gene="rimM"
                     /locus_tag="KPK72_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG2489347.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            16434..17129
                     /gene="trmD"
                     /locus_tag="KPK72_002133"
     CDS             16434..17129
                     /gene="trmD"
                     /locus_tag="KPK72_002133"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG2489348.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            17314..17664
                     /gene="rplS"
                     /locus_tag="KPK72_002134"
     CDS             17314..17664
                     /gene="rplS"
                     /locus_tag="KPK72_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG2489349.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            17985..18881
                     /gene="ylqF"
                     /locus_tag="KPK72_002135"
     CDS             17985..18881
                     /gene="ylqF"
                     /locus_tag="KPK72_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG2489350.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            19270..20562
                     /gene="brnQ"
                     /locus_tag="KPK72_002136"
     CDS             19270..20562
                     /gene="brnQ"
                     /locus_tag="KPK72_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2489351.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         8683 a         1939 c         3724 g         6336 t
ORIGIN      
        1 ttattccaat ctaattgatt acacactctt attagtataa aatttaatta tatataaatt
       61 agtaagaggt gtaaactaat gaagattaat aaattaccac atgcagaact aaaactaatg
      121 aagtatattt ggggagtaga tgatgtatta gcctcaagag atatcataga agcaatgaag
      181 ttgaagtacg attggaaaaa gagcacaaca ttgacatttt taaaaacttt agtagataag
      241 ggatttttaa ctacttataa ggtagataga tgtacgcatt atactatagc tataaaagag
      301 aaagattatt taaaagttga gacaaagagt ttttttagtt ttatgcataa caattcattt
      361 aagagtttta tttcagcatt acatgatgat gaagttttag atagtaagag cttggacaag
      421 ttagaagaat actttaaaaa tttgaaagaa ggagatattg atgactaata aagttatcag
      481 tatcttttta gtttgagaca attgagagta gcgttttgat ttaatgttag tgttatgtga
      541 tggtagaaca ttatataaag aaaagtgctt atttccttag tatctgatag taaaaaaaat
      601 atagaaaata ataccaatta aaatttaaag aaagatatga aatactacaa tagcttaaat
      661 catgtaaata tgataaagat attgaaatta taaaaggaat ataattttag aagaaatagt
      721 tgacagataa attaccttac tatattattg tattaataaa ataatacaat aatatagtaa
      781 ggaggtaata aatgaaatct aaaaaacata atattacaaa aggattattt atagtttaca
      841 taattatttt aacatggata atactattta agttgcaatt tgatatctca tctcttgaaa
      901 ccatgaatct tagaagcata aacttagtac cttttgctgg gtcacttata attaataata
      961 gagtagatat atcagagatt atattaaatg tggctatatt tgtaccattt gggatatatg
     1021 tgtgtatgct aaaagaagaa tggagcttta taaaaaaagt tatccctatt tttataacta
     1081 gtttagcatt tgaaacactt caatatatat ttgcactagg tgcaagtgat ataacggatt
     1141 tgataggaaa cacactagga ggaatcatag gaatagctgt ttttatgcta ttatcgaaaa
     1201 tatttaagaa taataccata aaaattatca atgtattggc attgattgtg acaattattg
     1261 ttgtattatt tttaggatta gttatttttg caaacttata gaaataagta tatatgttta
     1321 gcttaaaata gaacgtaaga tacagttaca tataaaatta tttacacata aaaagcacct
     1381 atacaggtgc tttcctaaat tattaaagaa ttattgtatc ttgaaactct tgtatgttca
     1441 attcttgatt ttctcttgtt ttttctaata aatctaacgc acaatttata caaactttaa
     1501 tttctgcatt ggtatttttc tcataataag ttatatattt atttctatat ttatctattt
     1561 cttttccaca acgactacac attactatta cgctcatatc ttagttcctc catctctaat
     1621 tattcttaat atattcaata gtatcacttt tctgaaaata atgattgtta aaactgtatc
     1681 aaatttagat ttacttttta ttataatgca ttttaataaa aaaataaagg ttatgggtaa
     1741 aatatttata gaaaaaagta tattattgaa tagattataa aattttatta ttttttagca
     1801 gtgctaaata cacattaata tctataaagt gacaaaataa aaaatactat ttaaatagat
     1861 attaaaaaat tgataccaag tatttgtttg ctatatgaat aacaatttat ttaggaactt
     1921 tactttaaca ctgtataata ataaagtaat tgctaaaaac gtttgatgat ttggatgtat
     1981 ttttagaaat tagaaaaata tatacttact gactaatgat aatcttatat gtattttagt
     2041 ttgagtgatt taagaaaagt gttttgaatg gggattattt tatagagtaa ataaatatat
     2101 aatagtagaa aattacatat aaagagtagt gtttatttca ttaccttctt tatctgttcc
     2161 gatagggtct tgtagagata cttgagtttt attttttttg ttagatctaa tattcattaa
     2221 aatctcattt tcaatacatc tagaagcata tgttgctaat cgagttcctt tatctatgtt
     2281 ataagtttct atagctttaa ttaatcctat tgtacctatg gatattaaat catcctgatc
     2341 ttccgttgaa ttattatatt tctttgctat ataagctact aatctcatgt ttctttcaat
     2401 taatgtatct tttgcacttt tatcgttttc tattttaaat ttagttaaat actcaatctc
     2461 ttcttcaggg gttaagggtt tttcaaaaga tttaagagct gccaataggt cacccccttt
     2521 tcaacggtca ctttaaatta tatgaaggag acttatccaa gttgactgtt atctaataaa
     2581 ataatatgca tatttttaga atttaaaaca tatatttgca aacttttgaa tggaatatga
     2641 cataaagagt gggaatttaa atttaagttt ttgttgtata tatatgaaac aaggcgaaga
     2701 aattattgat ttataaagta taaattggaa ttgaggtgtc aataaatata aaaggaaata
     2761 gtatttatat atgttttggt gttgaagtaa aagattaatg caaagtatta gagtctataa
     2821 aaatatgaat ataggaggtg taattttaca atatttaaaa aagtaaatgt aattaattgt
     2881 ttgtataaaa ttgattacaa gcaaaagtat cagtcatagt taatactgtt aaggatgaag
     2941 gtggtgtgtt aatttatata tgtatgggaa gaaattttga ttacaatgaa aaaacttaca
     3001 aaagatattt acgaaaaaac ttatagttaa atgtttatat ataaaatatt atttaggggt
     3061 tgtttttatt attgaaaata agaaagagtg gttttatatc aatcgaatgt ataataagta
     3121 ttgctatatt atatgtggct gtttatttag tttctacatc attgtataat tgttatagtt
     3181 ttatcagtag aaatatatct gacagagaaa tgttaagtac agcaaaaaaa tatatagaag
     3241 atgagaagta tagagtacaa aatagtaagt atgagttaat tgaagataag atagaaaaaa
     3301 attacataaa tggatatgaa attaacagta gaatagagca aattttagat tattatcaat
     3361 gctatgaaat aaatatagag ataaaaaatg aatttaaaaa actgaggttt aatagctatg
     3421 ttactagaaa ataaaggctc tatattaata ataacattga taatattttc tattataagt
     3481 actgtgtgca ttatgtgtat aggtttaatt tatacacata ataatatgtt tgagttagga
     3541 tacaaagaca ttcaactaaa agaaatttct ttaagtggaa tagaaatatc agtaagtaat
     3601 gtactttctt gtgttgagga agctattgat gtatctaata atgaagacga atttagagaa
     3661 tatttcttag gaaataatat gattgaatgt ataaaaaaaa taaaagatac atcttatagt
     3721 agtttagaag aactttcttt aaaagtagaa aataatacta tttatgacag aggagatttt
     3781 tttgaatttg atatagaaag taatgttaga gaaaaagaat ttttaaaagc aataaaagta
     3841 agagtgaaaa tcaaaaatcc atggatagga attgatattg aagattatat ggataaatct
     3901 aataacaata aaattgatga caaaacattc aataattata aagcatctga aaataatgaa
     3961 attctaaatc aatttaaaaa tgaaagtgaa aatataagga atcaagaggt tgttgaattt
     4021 acagaagaag atgaagaaga acttaaacaa aacataattg aaaatataaa gaagaatttt
     4081 gatgaaaaaa aactagtaca ggtatataag tataggggtg tatagctttg aaaaaagaag
     4141 gaatgtgact gtaatagaat taatagtatc tataagtata atactcttga tagttacatt
     4201 atcttttcca aaagataatc ttgaaaatca taaaataaat ttatttgcac gtcaactttg
     4261 ttcagatata aggtatgtta ggcgaattaa tatgtttagc aactacaata catatatata
     4321 ttatataaat caaggtaaac acgaaggtta tgtattgaga aaagatggta aaaacattaa
     4381 atcaataatg ctacctaaaa atgcaaaaat agctaggagt attgaaataa taaagtttaa
     4441 atctgatggg tcgcctcaaa aggcgtctac aatagaggta tataataata gattaagaaa
     4501 aactataaca ataacacctg taagtggaag agtacttctg aaagagggaa aatatgaaac
     4561 ataaatatgg ttacttgctt ttggaaagtg tagtatcatt gtcttcaatg gtgataatta
     4621 tactagtttt atattctata tttttgtcta caataaactt aaagttaaaa gtagaagata
     4681 agatagaact acaacaacaa agtcttgaaa tcataaaatc tatggaagga attattagca
     4741 attctatggg aataatgaat atatctaatt atgaagaaac atttaaaaag acaacgtcta
     4801 taaaatgtag atatgtagat gaaaataata atgaagaagg cataagtaat aaagaaatta
     4861 tattgaatga aagacgaaat aagttatttg taaattcttt aaatggcgaa agtagtcaag
     4921 caggtggtta tgaaattgga gattatgtag atgaaatgta tgtatcaatt actaataatg
     4981 gtcaatatgc taatattaaa ttgaaattat caaaacgtag tcaaaaatat gagactgatt
     5041 tcaaaataaa agtatggaat tttagtgaaa gtatataaaa atacataaat ttaagagaaa
     5101 gaaatgacat caataagtca tataaaatat aattattgag aactatataa ctaaataata
     5161 tcaatatgta aagtgtatag attaagaata tagaggataa taataaaaat atagaataag
     5221 taatttgcca aatacttgtt tatattgtat aacttatgat attatgatat tagtaaaaat
     5281 ttgtaatcct gacttgacag aattcaagta agagatataa ttttaaaggc atagtaatgt
     5341 aaaatttact aaaaaataag aaatttattt taatatggag gaacaaaaaa tggtatcagc
     5401 aggtgatttt agaaaaggtg ttacatttga aaaagatgga caaccatgtt tagtagttga
     5461 ttttcaacac gttaagccag gtaaaggagc tgctttcgtt agaactaaat acaaaaactt
     5521 aaaaacagga gctataagag aagaaagttt caatccaagt gaaaaattcc ctaaagctgt
     5581 tatagataca agacaaatgc aatatctata caatgatggt gagttatatt attttatgga
     5641 tcaagaaaat tttgaacaaa taccattaaa ctatgagcaa gttgaagatg caattaagtt
     5701 cttaaaagaa aatgaagttg ctacaataag attttaccaa ggacaaccat tccaagtgga
     5761 agcgccaaac tttgcagagc tagaagttac agatacagag ccaggtataa aaggtgatac
     5821 agcaagtaat gtaactaaag cagctacagt tgaaactgga gcagttgttc aagttccatt
     5881 atttataaat actggagata aagtaaagat agatactaga acaggtgaat atttatcaag
     5941 agtataaaaa tttatgtata taaaagctta tatgtcttaa taatataaga tatatgagct
     6001 tttagtttat atataaaaaa gaaagcgagg ggtgttacat gaaacattta tatgaagaag
     6061 ttgcatactt aaaaggccta gcagaaggac tagaaataag tgctgaaagt aaagaaggga
     6121 aaatgataca taaaatagtt gatgcattag aagtatttgc agatgctatt gtaacattgg
     6181 atgaagaaca agaagaactt caagattttg ttgaatctat agatgaagat ttagcagatt
     6241 tagaagaaga tattgctgac atggaagaag atctttatga agaagatgac gatgaggatg
     6301 atgaggactt cagctatata gaaatggaat gcccaaattg tggagagtta gtagaaatag
     6361 acgaagattt gttatatgat gatgaagtag atgttgtttg tcctgattgt aaggctgtga
     6421 tattatcatc agaagatgat tgtgatgatg attgtacatg tggtggttgt tcaagttgtg
     6481 atgatagaga gtaaaaattc ataagaatta aaactgccta tgatgcctat gagatatttt
     6541 gcttcatagg tagttttatt tttatgagga agggtattat gtattttggt tttgcaatac
     6601 ttggacaaaa atagtacact agaatttata gaataggatt ttggagggat gtaaatatga
     6661 aaataagtaa aaaattacta gataatatac aaagatttga aaatgttata aattataaat
     6721 ttaaaaataa agaatatata ctagaagctc ttactcatag ttcatattct aatgaaaata
     6781 aaaaatataa ttttaacgaa agattggaat ttttagggga ttcggtttta ggcatagtga
     6841 taagtgatta tttatttaat gaagaagcta atctgcccga aggggaacta actaaactta
     6901 gagcaaatat agtttgtgaa gattcattaa gtgaagtagc taatgatata aatttaggta
     6961 ttcacatgtt attaggtaga ggagaagaag caacaggagg aagacatagg acttcaatat
     7021 tagcagacgc atttgaagct gtaatagctg ctatatatct tgatggaggt tttgaaagtg
     7081 caagacagtt tatacttcat catatggaaa atataattta tgattcaaga aaaggaaata
     7141 tatttagaga ctacaaaacg catcttcaag aggtgttaca agggaatgga gaaaataata
     7201 tctggtacag gcttatagaa gaaaaaggac cagaccataa taaaagattt gtaatggaag
     7261 ttggaattaa tgacgatgta cttggtatag gagaaggcaa gagtaaaaaa gaagctgaac
     7321 agttagctgc taaaatagct ttaaagaaaa aattatggga aaaataaata taatatgtaa
     7381 aaatataaaa agaaatattt atattagaag gatgttgtag atgaaaaaga gaattatacc
     7441 tatatttgtt cctcacaaag gatgtcctca tgattgcata ttttgtaatc aaaaaaaaat
     7501 aacaggtgtg agtactgatg ttactagtga agatgcaaga aatataattg aggaatgttt
     7561 agagactata gataaagatg cagatgtaga aatagcattt tttggtggta gctttactgc
     7621 tatagatatt gatatacaaa aaagtttatt atcagttgct aaagaatatg tagaaaaagg
     7681 tcttgtaaaa gatattagaa tgtctacaag accagattgt attgatgaag atatattaag
     7741 tatgttaaaa gaatataaga caagtataat tgaactagga gtacaatcct tagatgaaaa
     7801 agtattatta gatagtataa gaggtcatca atctgaaatt gtatacaaga gttcaaagat
     7861 gataaaaaat agtggaatca aactcggatt acagatgatg attggtcttc cagctgacac
     7921 agaagaaaag tgtatattta cagcaaaaaa atttgtagag ctaaaaccag attgtgttag
     7981 agtctatcca actttggtag taaaagatac aggtcttgaa aagttaatgg aacaaaataa
     8041 atataatcca tttactcttg aagagagtgt aaatatagta aaaaaagttc ttgtactgtt
     8101 ttatgttaat aatatcaatg taataagagt tgggcttcaa gctactgatg atatacaaat
     8161 tggaaaagct gttttagcag gaccatatca tcctgcattt agagagttgg tagaagcaga
     8221 tatgataaaa gattatttag aatttgttat tttgcaaaat aaaaatataa agcaaatgtt
     8281 ggtcaaatct aataaaaaga atatttctaa aatcatagga aataaaaaaa caaatgttaa
     8341 atatatggaa gaaaaatttg gtgtgctttt aaaaacacaa gaatcagatt tagatataaa
     8401 tcaattagaa attgtgttag atgggaaaag tttaattagt gctaatatga gagacattca
     8461 tagaaaattg tatgacattt atgaccttta gttttaataa tgaagggaga gaaaagattt
     8521 gtatttaaaa agactagagt taaagggatt taaatccttt ccagtaaaaa cagatattat
     8581 ttttaaagaa ggaataacag ccatagtagg accaaatggg agtggaaaaa gtaatatatc
     8641 tgatgctgta agatgggtat taggagaaca gagtattaag agtcttagag gtgataaact
     8701 cgaagatgta atatttgcag gtacagatac aaaaaaacct atgaattatt gtgaagtggc
     8761 tcttactatt gataattctg aaaatcaatt ggaattagat tttacagaag taactataag
     8821 aagaagggct tatagaaatg gtgaaagtga attttttcta aataataaaa gttgtagatt
     8881 aaaagatata aaagaagtct ttttagatac tggtattgga aaagatggtt attcaatcat
     8941 agaacaagga aaagtagatg aaatattaag taataatcct ttaagcagaa gaaaagtttt
     9001 tgatgaagct tgtggcatat caaaatatag atataaaaaa caggaagcag aaaggaattt
     9061 aagtaataca aaggaaaatt tagaaagaat agatgatgta tatatagaaa ttgaaaatca
     9121 attaaagcct ctttttaatc aacagacaaa ggcaaaaaag tatttggaaa taagtgaaaa
     9181 attaaagaca cttgaagtaa atagctttat aagagaaatt gaaggaatag aaaaagaatt
     9241 aagtgaggtt aatgaacatc gtaaggttat tgaaaaggag ctcaatgaaa aagaagaaca
     9301 aaaaaatgtt gtagagaaaa agcaagaaga tattaataaa gaggtagagg tattacaaga
     9361 tgtaattgaa aaatctgtag attatataaa ttcaattaaa ggagttatat caaaaaaaga
     9421 gtcccaaata aatttaatta aggaaagaat aagaaatttt acgaatgaaa ttagtagaaa
     9481 aaatttagaa ataaatgata taaaagagaa gctaaatgaa aataagcaat acataaaaga
     9541 attagaaagc aataaattat caggtagtga agaactatct accttgcagg aaaatataaa
     9601 agtattggaa gcaagtaaag ataaacaaaa aattaagtta gaatccctaa ataatgagat
     9661 agaattattg aaagaaagta taattgatat attaaataag aaacaagagt ttagtaataa
     9721 gctgtccact ttaaacgcaa ataaagaaaa tatgaatatc agagatgaaa atataaattc
     9781 agagataaca gagttaaaca aaaatattga aataaaatca tctgaattag atactataaa
     9841 taaagaattt aatatgcaaa atgaaaattt aaagaatgta aataatagac ataaagaatt
     9901 atcaactaat ttgcaggatt caattagtga acacaataaa ctagaagatg aaatacagaa
     9961 aagtaaatat aatttaaatg gatataattc aaagttaaat gtctatatag atatggaaaa
    10021 tcattatgaa ggatttaata gaggagtaaa agaagtacta aaaaataaaa atttaaaagg
    10081 agttcatgga gcattaggac agattataaa tgtacctgaa aagtatgaga aatctataga
    10141 agcagctctt ggtgcttata tgcaaaatat aataactgat aatgagttta gtgctaagtc
    10201 tgcaataaac tatctaaaac agaataattt aggtagagta acatttcttc cattaaatat
    10261 aattaaatca aataaaataa gtctaggaaa tttaaaagca aatactaagt tcataggaat
    10321 agcaagtgat ttaattacat ttgatgaaaa gtatagaaat ataattgaaa atatacttgg
    10381 aagaacaata cttataaata atatagatga gggcataaaa tttgcaaaag aaactggtca
    10441 taggtttaaa atagtaactt tagatggtga aatcttaaat ccaggaggtt ctttgactgg
    10501 gggaagtcta aaaactaatg ggaatatact atcgagaaaa agatacatta atgaatatac
    10561 agaaaagata agtaatatca agaatgaaat ttcacatcta gaattaaaaa gagaatcttt
    10621 agacaaagat gtgaaaaata tcaaaaatga aattgattca catgaaagta aaataaaaga
    10681 tttagaaaaa agtataataa taaaaagtac aagtataaag aatgttgagt ctgaaattga
    10741 atcggtaaaa tgtagtatta ctaaattaga aaatgagaag aatgatttaa attctaacct
    10801 aaattataca cttgaaaaaa gtgatgatgt tagaaaagat atggaagagt tagatgattt
    10861 atacaataaa aataaagaaa aaattgatgc attgaatgag gaaataaaaa gatacaatga
    10921 tttatatgat aaagaaaaat ctgaatttga tgaattgaac ttaagtttag tgaagaaaac
    10981 agaagtttac aatagtatag ttagagatat aaaaagaata tctggtgaaa attgtgagct
    11041 agaagaaaaa aataaacaat tagaagagtc attaaattat gaagaacatg aaataattaa
    11101 attacaagat tctatactca ctgaagaaaa agaaaaagaa aatttaacaa aacaactagg
    11161 ggatagtaat agaaatcttg aaactagaaa aatagcaaaa gatgatttaa aaaatagttt
    11221 tgatgaaatt aataaagaat taaaaactat agatagacag catatagaac ttaaagaaag
    11281 tttatttaaa gttggtggaa gattagaaag attaaaaact agtcaagaca catatataaa
    11341 taagttgttt gagcaatatg atatgacctt agtacaagca ttggaaatta aagatgaaga
    11401 tttggatata gatagaaaat tcttagaaag tctaaagaga gaaataagaa gtcttggaaa
    11461 tataaatata gactctataa aggaatatga ggaaataaaa gaaagatacg atttttatag
    11521 cgaacaaaag caagatttag aagagtctat ggaagagata gaaaaactta tacatacttt
    11581 agaagaaaat atgaaatctg aatttgaaat taagtttgaa gaaataagta aaaattttaa
    11641 atatgtatat aagagattat ttggtggagg ttgtggagaa ttaactattt tagataaaga
    11701 aaatttatta gaaagtgata tactaataac agctcaaccc ccaggtaaaa aaatgaaaaa
    11761 tttaaaccta ttatctggtg gagagaaagc tttaacggca ataagtatat tatttgctat
    11821 attgattaca aagccaacac cattttgtat attagatgag attgaagcgc cacttgatga
    11881 cgcaaatata tttagatttg gagaattttt gaaggattta tctaaagaga ctcaatttat
    11941 atcagtaact cataggagag gaactatgga agctgctgat tatatctacg gagttacaat
    12001 gcaagaaaaa gccatttcaa aagttattag tttaaaatta aaagaagctc aggaaataac
    12061 agatattata taacttagat tgacatagga ggaataacag tgttaaaaaa attatttggt
    12121 tttggaaaag acaaggaaaa ggaaatagaa aaaaaagatg ctgaagaaga aatagaagtt
    12181 gaagacagtg ttgataattt agaaaacttg gaagaaacta ttttttcagg acttgaagaa
    12241 gaagtgatag ataaagtgga agatgttgaa gaaaaaaatg aggaaagtgt caataatgaa
    12301 ggcactgaag aaatcgaaaa ctttgaaaaa atagaagttg agatggatag taatggaaaa
    12361 gaagtagaaa atatttcaaa taatgattct caagaagaga taatcgaaga attagaaaat
    12421 tatgatgaaa ttgagtctaa agagatagaa gataaagaag ataagaaagt taacttgttt
    12481 gaaagactaa aacaaggatt gacaaaagca aagcaaggaa taactgatag aatagatgaa
    12541 gtcttaaaat catatacaaa gatagatgaa gaattacttg aagatttaga ggaaatttta
    12601 ataacagcag atgttggtgt aaatacaact atggatataa ttgaaagact aagagataaa
    12661 ataaagcaaa aaggtattac tgaaccaata aaggtaagag aagaacttaa atctatagtt
    12721 gaagatattc ttacaaatga aaactctaca ctagatatag aaccagcacc atgtataata
    12781 ttaatggtgg gtgtaaatgg ggtcggtaag actactacaa taggtaaact agcaaataga
    12841 tacaaaaaag atggtaaaaa agttttatta gcagcagcag atacatttag agcagcagcg
    12901 acagagcaat tagaaatttg ggcaaataga accaatgtag acataataaa acatcaagag
    12961 ggagcagacc ctggagctgt agtatttgat gctataaaag ctgcaaaagc tagaaaaaca
    13021 gatgtattaa tatgtgatac agcaggaaga ttacacaata aggctaatct tatgaatgaa
    13081 cttggaaaag tatttaaaat agtagataga gaatttccag aagcaaaacg tgaagtactt
    13141 cttgtggtgg atgctacaac aggtcaaaat gcagttgttc aagcaaagac atttaaagaa
    13201 gttgcagata taacaggtat agtacttaca aaacttgatg gaactgcaaa aggtggagta
    13261 gtccttgcag taaaatcaga agtagatgta cctgtaaaac ttattggagt tggagaaagt
    13321 gtagaagacc tacaagattt caatgcaaaa tcattttcag atgcattatt tggtaactaa
    13381 tgttctttta aaaataatgg ttgacaagag tgttgttatg atataaaata caatagtgta
    13441 aagtaaataa cctttacagt atactgctag gaagtgtaag atatgaatat agaaaaaatg
    13501 gtcgaaattg gattgttatt tgaacagtat aaagaattat taactgacaa gcagaaagaa
    13561 atagttgctc tatactatga agaagattat tctttaggtg aaattagtga aaatctgaat
    13621 gtatcaagac aaggtgttta tgatacctta aagcgttcag aaaaaatact aagagattat
    13681 gaagaaaagt tacacttagt atcaaagatt caagagcagg aaaaaaatat taagattata
    13741 aaagataaaa ttattgacat taaagaagat ttattgcaca atagagattg tgctaattta
    13801 atccctaagc tagaaaatat agaagatgta tgtagggaga tgataaaatg atatttgaag
    13861 gattgtctga taaactacaa ggggcattta gtaagttaaa atctaaagga aaacttacag
    13921 aagtagatgt aaaaaatgct atgagagaag ttaaacttgc tcttcttgag gcagatgtta
    13981 actttaaagt agttaaagat ttcataaaaa aagttcaaga gagatgtgtt gggcaagaag
    14041 ttatgcaaag cttaactcca gctcagcatg taatcaaaat tgtaaatgaa gagcttacaa
    14101 gtttaatggg agatgtacaa agtaaagtaa tgatttcctc aaagcctcct acaattttaa
    14161 tgatggttgg attgcaaggt gcaggtaaga ctactacagc aggaaaactt ggaggatatt
    14221 ttaaaaagca aggtaaaaaa cctctattag tagcttgtga tatttataga ccggcagcta
    14281 taaagcaact tcaagttgtt ggagaaaaat tggatatacc tgtattcaat atgggagata
    14341 aagaaaatcc agtaaatatt gcgaaagcag gtttaagcca tgcgattaaa aatgctaatg
    14401 atttagttat tatagataca gctggtagac ttcatataga tgaagtactt atggatgagt
    14461 taaagtctat taaatcagaa gttaagccac atgagatact tttggttgta gattctatga
    14521 caggtcaaga tgctgtaaat gttgcagaaa gcttcaatga ggcacttggt gttgatggag
    14581 tagtattaac taaattggat ggagatacta gaggtggtgc tgcactttca ataagagcag
    14641 ttacacaaaa gcctataaaa tttatgggga tgggagaaaa gctagatgat atagaacctt
    14701 tccacccaga tagaatggca tcaagaatat taggtatggg agatgtctta agtttaatag
    14761 aaaaagcaca agaaaatata gacttagaga aagctaaaga attagagcaa aaaattaaaa
    14821 aacaagagct tgattttgaa gatttcttac aacaaatgga acaaatacaa aatatgggac
    14881 ctcttgacaa gatacttggt atgatacctg gtatgggaaa tgtaaaagac caacttggag
    14941 atgtagattt gaatggcaaa gaaatgaaga gaacaaaagc cataattcag tcaatgacta
    15001 catatgagag aagaaaccca ggagtattaa atgcttcaag aaagaaaaga atagctaaag
    15061 gcagtggaac tagtgtacaa gatgtaaata gacttataaa acaacttaat gaaatgaaaa
    15121 aaatgatgaa gatgtttgct ggttcacaaa agagtatgaa gaaaaggggc ggcttaggcg
    15181 gtttaccttt ttttaaataa aatataaaaa agtaaaaatt attcggaggt gactgtaatg
    15241 gcagttaaaa taagattaaa aagaatggga gctaacaaaa aacctttcta cagaatagta
    15301 gtagctgatt caagagctcc aagagatgga aaatttatag aagaaatagg atactataat
    15361 ccaatttctg aacctaaaca agtaagaata aatgatgaaa aagctataaa atggttagct
    15421 actggtgctc agccaacaga agtagttaaa aaattacttg taaaaaacgg agtaatagaa
    15481 aaattcgaag cttctaaaca agcaaaataa ttttttcttg gcaattagtt taagtaaggg
    15541 ctcattgcta aggttacttt aggagatgaa atatatgaaa gagctagttg tagatatagc
    15601 taaggctcta gttgataatc ctgactcagt tgttgttgag gaatttgaag ataatgatgg
    15661 tatcgtctta aagcttacag ttgctcaaga ggatatgggt aaggttattg ggaagcaggg
    15721 aagaatagct aaagctataa gaactgtcgt aagatctgtt gcaaatagag aaaatataaa
    15781 agtttctctt gagatagtat aaaataggag gattaggtat aatgcctagt cctttttata
    15841 taaatgcgta gtttaagatt ttatgatgat atgaccacat aagattaaat tttttaagaa
    15901 ggtgataaca tgaaagaaaa attaactcat tttaaggtag gtcaaatagt aaatacacaa
    15961 ggtttaaaag gtgaagtaag agtttatcca cttactgatg atatagagag gtttgatgaa
    16021 ttaaaagatt tctatctggg aaaagattta aataataaat tggctgttga aaaagtaaga
    16081 tacaaaggtt ctatggttat aatgaagata aaaggcatag acagcataga gaaagcagaa
    16141 aaactaaaaa ataaatttat gtatgtatct agagaagata gcagagagct tgaagaaggc
    16201 gaatttttta tagcagatat gattggaatg gaagttttaa ctgtagatgg aaaatatgta
    16261 ggaatactag aagatgttct tcaatactca gcaaatgatg tctatgtagt aaaaggtgag
    16321 gaagataaag aatttatgat accagctata aaaaaatttg tacctacaat agatattgat
    16381 gaaagaaaaa tgattataga ccctattaag ggaatgatag attaggtggt aatatgagat
    16441 ttcatataat gacacttttt ccagaaatat ttaattcgta catggatgaa agtataatga
    16501 agagagcagt tgaaaaaggt ataattgagg ttcatatata taatataagg gatttttcta
    16561 ataataaaca taaaaaagta gatgattatc catttggagg aggagcgggg atggtaatga
    16621 ctcctcaacc tatatatgac acatataagc atataataac aactcataat ataaataacc
    16681 caagtgtaat ttatcttact ccaaaaggta aggtatacaa tcaatctatg gcaaagcaga
    16741 tgtctttaaa agaagatata atactactat gtggtcatta tgaaggaata gatgagagaa
    16801 tcattgattt aatagttact gatgaaattt caattggaga ttatgtatta actgggggag
    16861 agctcccagc acttataatg atagattcta tatcgagatt aataccaggt gtattaaacc
    16921 aagaagaatc atttgaagaa gaatcattta aagacaactt attggaatat cctcattaca
    16981 ctagacctag agattttgaa ggattaaaag taccggaagt gcttttatca gggaatcata
    17041 agaagataga tgagtggaga agagaagaat ctataaggat aaccaaagaa agacgatttg
    17101 atttatataa aaagtctaat gaaaaataaa aatataatga aaaattaata tatatgtata
    17161 caataaagta cctataaatg gaattaaaaa cataaaagtg cttgtaataa ctattgaagt
    17221 atagtataat ataaaatgtt gaaaatacgg gcgttcctct gtttacactg gtcgggtagt
    17281 taagaacgtc tatgatatct aggaggaaga aagatgaatg aaatgctaag agcaattgag
    17341 caagaacaat taaaaaatga agttcctaat tttggacctg gggacacagt taaagtacac
    17401 gttagaatta tagaaggaaa aagagaaaga atacaagtat tcgaaggtgt agtattaaag
    17461 agacaaggtg gaggagcaag agaaactttc acagttagaa aaatgtcttt caatgttggt
    17521 gtagaaagaa ctttcccagt tcactctcca aaaatagaaa aaatagaagt tactagaaaa
    17581 ggtaaagtaa gaagagctaa actaaactac ttaagaggta gagtaggtaa agctgctaag
    17641 ataaaagaag ctagaaataa ataattaaaa actttactta taaatggggg gctgatttat
    17701 cagtcctctt tctattttaa tgaaaattac agcaaataat tatactagaa ataaatagaa
    17761 atatttgttg aaaatataca ttatttatct atgaatttat agcttttaaa atgaaaaata
    17821 aaattggtaa atattgcatt atattttgta acaatattaa aaataagaga acagtattat
    17881 gtaaagtatg acgttaaaaa gatgacaata ttatagtttg ttaagataga tttttttagt
    17941 ggaattagga tataataata taatattaat aaggagtgat acatgtgtca aatgaatacg
    18001 aagagtatct tatgaatgac aatctacata taaactggta tccaggtcat atgaaaaaaa
    18061 ctaaggagtt agtaaaaaat aacttaaaat tgattgatgt agtaattgaa cttttggatg
    18121 ctagaatccc gtttagtagt aaaaatccag acatagacag gcttgtagga gataagccaa
    18181 gagttgtcgt acttaataag agtgatatgg cagataggga taaattgaat caatggattg
    18241 aatactataa gaaaattaat atcaaagcta taccagtaga tacaataaaa ggcgttggta
    18301 taaataagat aatcgaagaa tgtaaaaatg taactagaga aaaaatgagt tctttaaaag
    18361 acaaagggag aaaagaaaga gctatacgaa taatgatagt tggagttcca aacgttggta
    18421 aatcgtctct tataaacaaa ttaacaggta gaaaaagcac tcaaactgga gataagccag
    18481 gagtaacgaa agggaaacaa tgggttagat taaagggaaa tcttgaactt ttagatacac
    18541 ctggaatact ttggcctaag tttgaagacc aagaagttgc attaaattta gcatttagta
    18601 gagctataaa ggatgagatt ttagatactg aaactttggc tttaagactt atagagaaac
    18661 ttatgaaaat agaacctgag aagttgaaag ccagatataa gttagattgt ttaggagaaa
    18721 ctccaattga aactatggat atgattggcc ataaaagagg gtttataaca ggtaaaaaag
    18781 aactagatta tacacgcata gccacaactg tattaaatga atttagagat ggaaaaatag
    18841 gaaatataac attggaagtt ccagaaaacg ttaaaagata aattttaaaa caaaatcaaa
    18901 aatttaatgt taaaaattta aaaaagcctt ttagcaaagg cttttttatt tttttgaaat
    18961 atagacaaat atctccatat aaaagtataa attaggttag aaaaccttta aaaatattaa
    19021 tttaagacaa atatacctaa tattttgaaa attaaaatga gtgtgattat ttaaaatttt
    19081 agcatattga cttgcttgtt tcgtataata tataataagc acaaataaat acaatatatt
    19141 aaaaatttaa tttatttaat tttattaatt taataataag ttcttattgt ttaattcaac
    19201 ttacttatgc aagcgtaaac aatcgagaca ttttattaat ttaataaggt taaaaagggg
    19261 gattttataa tgggtaagac caaggatgtt atagtatttg gatttgcgtt atttgcaatg
    19321 ttctttggag caggtaactt aatatttcca ccttacttgg gaataattac aggtccagaa
    19381 tggttaattg ccttcttagg atttacgttt gctgatgctg gattggcact tctagcagta
    19441 atggctacag ctaaattcga tggaaacgta gttgaaatgt ttaaaagatg tggtatgaag
    19501 ttagggattc ttataggttg tgcagatatt ttatgtatag gcccattctt agctatacca
    19561 agaacaggtg caacaactta tgaaatgggt ataatgcctt tgtttggaac ttcaattcca
    19621 gtgttattat tctgtatact tttttttgcg atttcatatg tactaacaat aagaccttct
    19681 aaggtcgtag atatagttgg acagttttta actccagctc ttctaatagc tttagcattt
    19741 ataattataa aaggtattat atcacctctt ggagatatag tagataaacc tatgatacct
    19801 aatgtatttg cagaaggtat aggtcaagga tatcagacaa tggatgcttt tgcagcaata
    19861 gcacttgcat cagtgctaat agtatcttta aatgataaag gatattctac tattagtgac
    19921 aagttgaaaa tgattggtaa ggcgggtgtt cttgcttgtg gaggattagc tctagtttat
    19981 ggaggattat gtttccttgg ggcaactgta tcaacaatgt atggaacaga tgcagtacaa
    20041 tcacaagtaa tagtaaatat aactgaagga ttgcttggaa atgttgggaa agcgatttta
    20101 gcagtagtag tatcattagc atgtttaact acatctatag gtcttacatc tgccacaggt
    20161 caatatttct ctagacttac aaaaggtaaa ttgagttatg aaaaaatagt attagctgta
    20221 tctgtattca gtgcagtagt agcatctttt ggtgttggaa ctataataaa gatagcatct
    20281 cctatactaa gtatagttta tccaccatca attgttttaa taattttagc tttcttcaat
    20341 gaaaaaatta agaatgataa tgtatacaaa ggagcagtat atatgtcatt gcttgtaagt
    20401 atactaacag ttatatctag ttatggagta gctgttccag tagtaaattc tttacctctt
    20461 aattcattag gatttaactg ggtggtgcca gttataatag ctggaataat tggtaatttt
    20521 ataccatcaa agagtcaatc taatacactt ggaacaaact aagttttgtt taattaaaat
    20581 ataatcaatt gtaaataaat gagttaacta aaagtaaaag gagttatttg tacaaataac
    20641 tccttttatt tttagattct atatttaatt atttgtattt aa
//
LOCUS       DAEMHZ010000061        20084 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000061 DAEMHZ010000000
VERSION     DAEMHZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.061"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            467..979
                     /locus_tag="KPK72_002137"
     CDS             467..979
                     /locus_tag="KPK72_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489352.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(1805..2902)
                     /locus_tag="KPK72_002138"
     CDS             complement(1805..2902)
                     /locus_tag="KPK72_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2489353.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            3195..4721
                     /gene="glpK"
                     /locus_tag="KPK72_002139"
     CDS             3195..4721
                     /gene="glpK"
                     /locus_tag="KPK72_002139"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2489354.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4762..5877
                     /locus_tag="KPK72_002140"
     CDS             4762..5877
                     /locus_tag="KPK72_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG2489355.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(6158..6922)
                     /locus_tag="KPK72_002141"
     CDS             complement(6158..6922)
                     /locus_tag="KPK72_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2489356.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            7181..7271
                     /locus_tag="KPK72_002142"
     tRNA            7181..7271
                     /locus_tag="KPK72_002142"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7215..7217,aa:Ser,seq:gct)
     gene            7359..8147
                     /locus_tag="KPK72_002143"
     CDS             7359..8147
                     /locus_tag="KPK72_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG2489357.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            8168..8908
                     /locus_tag="KPK72_002144"
     CDS             8168..8908
                     /locus_tag="KPK72_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2489358.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVESGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            9084..9458
                     /locus_tag="KPK72_002145"
                     /pseudo
     CDS             9084..9458
                     /locus_tag="KPK72_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9550..11010
                     /locus_tag="KPK72_002146"
     CDS             9550..11010
                     /locus_tag="KPK72_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG2489359.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFNAELENAIKK"
     gene            11063..14092
                     /locus_tag="KPK72_002147"
     CDS             11063..14092
                     /locus_tag="KPK72_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2489360.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYKTENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            14169..16235
                     /locus_tag="KPK72_002148"
     CDS             14169..16235
                     /locus_tag="KPK72_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2489361.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDIRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    16953..17163
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17389..18204
                     /locus_tag="KPK72_002149"
     CDS             17389..18204
                     /locus_tag="KPK72_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2489362.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            18232..18909
                     /locus_tag="KPK72_002150"
     CDS             18232..18909
                     /locus_tag="KPK72_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2489363.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            18968..19690
                     /locus_tag="KPK72_002151"
     CDS             18968..19690
                     /locus_tag="KPK72_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2489364.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         8013 a         2069 c         3548 g         6454 t
ORIGIN      
        1 caaaactgtt gtaaaaaata taactttatt taaaactttt ttttaatatg taaatatata
       61 tactaatagg gggtacttat ttttagaata ttcagtaaaa tgattttgat atactctcta
      121 tattttgtat gttaaaagta aataaattat tgttaaagaa ggtaaagact tttaaatttc
      181 ttatcatagg attttaagta aataagaagg attggagtaa ttaaaaattt ttattttgca
      241 aaaataagta tttatttata tggataaatt tgattttaaa tctataaata tagataaaat
      301 ctaattattt aacttcaact attcctcaaa aaagcaactt ttaaggaata gttgaagcaa
      361 aaagtttggt tacataatag taattttagt tacaagaatg aaactgtaaa acttaggatt
      421 ttctttatat aatttcattg agaacattta atttaggagg taagatatga aagtaaaaaa
      481 atttaaaagt ggtatagtaa tggcattaac agtaatggca attggagcat cattgacaaa
      541 tgtatcagct atggaattaa acaagagtaa agagtttgaa actaaataca atgaaatgaa
      601 gagggatttt caagaggaat atggaaaaga agacaaattt aatataacat tggatgatga
      661 atttacaaaa gaagaagtaa atggagctaa agggattata aagacaacta ataaaatgaa
      721 taatgaggtt gtagtattaa attattttga tgaaatgaaa tctggaattg aatttgagtt
      781 gaagccaaaa gcagaaaaaa atgcaatttt gagagatgaa tttcaacaag gttatggaaa
      841 ggaagataaa ttcaaagttg tgctagatga tgaatttaca aaagaagagg taaatggagc
      901 taagggcatt ataaagacta cagataaaga gacaggtgag gttatcgtat ataattactt
      961 tgatgagctt gaaaaataat gcttaaagat tactttgatt gaatgaaaag gtaattgtgt
     1021 tgagaatatt aaatgactga ataaaaacag ttgtatcaaa acaaacaata atagaatgta
     1081 agaatgattg tgtcgaaaat ataaaatgac tgaataaaaa acgattgcgt caaaaataaa
     1141 caataataga atgtaagaat aattatgtcg aaaatataaa atgactgaat aaaaacagtt
     1201 gtatcaaaac aaacaataat agaatgtaaa aatgattgtg tcgaaagtat taaatgactg
     1261 aataaaaaac gattgcgtca aaaataaaca ataatagaat gtaagaataa ttgtgtcaag
     1321 aatattaaat gactgaataa aaaacgattg cgtcaaaaat aaacaataat agaatgtaag
     1381 aatgattgtg ttgaaaatat taaatgactg aataaaaaac gattgtgtca aaaacaaaaa
     1441 ataattaagt gtaagaatga ttgtatcaag aatataaaat gattcaacaa aaaacgattg
     1501 cgtcaaaaat aaacaataat agaatgtaag aatgattgta tcaagaatat aaaatgattt
     1561 aacaaaaaac gattgtgtta aaaataaaaa acaattaaat gtaagaataa ttatgtcaaa
     1621 aatataaaat aaattaaaag aaaaagatta tactgaagga aaaagatttg aaataggggt
     1681 gaactttaca tattatagtt cacccttata caaaaatata aaatataatt tattatagga
     1741 aaatttaaaa aaatcagtct atttaattta tactgatata ttgtagggtt ttacaaacaa
     1801 actgttatat aacttttttt atctccaatt ttgattttaa gatatctaag aatttataac
     1861 aatcttctgg atatatttct cccatatttg ctacttggaa tatgttagca tctaggaatt
     1921 tgccttttcc tggatagaca acatagccac tttccctttc tagccctaat ataaactcat
     1981 ttacattgat ttctttaggt agaaatacag atgttactgt attagacata tacttatctg
     2041 gaagtaaaaa tgttagaccc aattcttcca taccattcct taaaatagca gaacattctt
     2101 tgtatctttc tattttactg tcatcttcca aaaactcagt taaagcttca ttaagggcaa
     2161 atattaaagt aacattaggt gtatttggtg tttgagaaga tgatttagca actttatagt
     2221 gtttataaag attaagatac acattcttac attgattttc tttaatattt tgtaaaatac
     2281 tctctttagc acaaaggtat gcagaacctg gaaatgctcc aagagctttg ccaccaacac
     2341 ctgttattat atctatatta aactcttcca catcaatata ttcacctgct gaggcactta
     2401 ttgcatctac aaaaaatatt ttgttatact tcttagcaag ttttcctagt tcagggactg
     2461 gatttatcat tcctgaacta gtttcatgga acaccatagc aattactttt atatcagaat
     2521 tttcaattat gtatttttct aaaatgtcta ggtccgggta ttctgcccat tcaaaattag
     2581 gtttatatgt ttttacatta taagtagata atatttcttc tagcctttct ccaaaaacac
     2641 cattactaac tagcagaact gcatcttctt tgtcaaagat agaagatagt acacattcat
     2701 ttacagatgt tccagagcca gatacaatta atgagtagta attagaagtt gcattatata
     2761 gtttattgat tttttgttgt gtatctttga ataaatccat aaactcattt cctctatggc
     2821 atatatcata gtgaagaagt gaatttctga ctttatctgt aaccattact ggtccaggac
     2881 tgaatagata tgtttttttc attgtaaagc ctccttaaaa agttaaattt aaatttttga
     2941 atgactgatt atgtgtaaat attaaaatgt tatgtgttat tttaactaga ttatagaata
     3001 aaataataaa tattgcaata atatttcaat aaaaatactt ttttaatcac tataattaaa
     3061 aataacaaaa aatattgtaa tgaatattgc ataaatccac atgatgatat aaaatataaa
     3121 taacttgaat agattatata ttcaaaaaat aaaaaagaat ttattaattc tgaaaataaa
     3181 gggggacatt taaagtgaaa tatgtattag caattgacca aggaacaaca ggaacaagag
     3241 cagtattgat aaacgaaaaa tcagagtttg tttttagcga ttatatggaa catactcaaa
     3301 tatatccaca atctggatat gttgaacatg accctataga aatttggaat aatacaaaaa
     3361 ctgtgagtaa taatgtatta caacaggctt ttgaaaatgg aatcaatgaa agtgatataa
     3421 aaggaattgg tatagataat caaggtgaaa cagtaatgtt atgggataaa aatacaggta
     3481 aaccacttta caatgctatt gtgtggcagt gcaggagaac ttatgattat gtggaaaatt
     3541 taaagaatat accaggttta gaaaataaga taatagataa gactgggtta attatagacc
     3601 catatttctc aggtacaaaa attaaatggg taatagataa tgtaaaaggt gtaaaagaaa
     3661 aaattaaaaa tggagaagta cttgcaggaa cattagatag ttggttaata tggaaaatga
     3721 caggaggaaa atctttttta actgatgtat ctacagcagc aagaacaatg ctttttaata
     3781 taagagatat ggattgggat agagagttgc ttgacattat aggagtacct agaagtattt
     3841 tagcagatat aatgcctacg agtggtgatt ttggaactac agataaggag gcattttgtg
     3901 gggtttctat aaaaattact gcttcaatgg tagaccaacc agcagcttta tttggcaatg
     3961 gatgttttga accaggaatg gttaaaaata cttatggtac aggatgtttc ctatatatga
     4021 atataggtga ggaactaaat atagcaggta atggtatttt gacaactgtt gcttggaaaa
     4081 taggagataa ggttacatat gcttatgatg gtggagtgta tatagcaggt gctgcaatac
     4141 aatggcttag agatggcatt ggagttataa aaaattattc agaaactgat gacatggcaa
     4201 attccatatc aagtactgga ggtgtatatt ttgtaccagc atttgcaggt atagcagcac
     4261 cttattggga ccaatatgct agaggtacaa tggttgggat aacaggaagt actaaaaaag
     4321 agcatatagt aagagcgact cttgagtctg ttgcacttca agtcaaagat gttgttgatt
     4381 ctatgaactt agcctcaggg aaaaaaataa aaatgttgag agtagatggt ggaattacta
     4441 aaaataactt tacaatgcaa tttcaatccg acatattagg tataccaatt gaaattgcag
     4501 aaaatcctga gacgacagta cttggagtag gatatatggc aggtctttct tgtggtgtct
     4561 ggaacagtat tgatgaaatt agaagtgaat ttacaataag caaaaaatat attccaaata
     4621 tgactactca aaaaagagaa gaaatattac ttggatggaa ccaagcagta aaaagagcta
     4681 tgaattggga aaacgatacc aaaaaggtgt gtgctaatta agttacaata gagtatttta
     4741 catgtatagg aggaattaaa aatgcccttt gataaaatta tattatacat aatggctgtg
     4801 ggagtgcttt taggagcgtt agatagaata attggaaata aatttggact tggtgagcag
     4861 tttgaagaag ggtttaattc aatgggtcca ttggctttag gaatggttgg tatagtttgt
     4921 ttagctccag taatttcaga tgtactaggt cctataataa taccaatctt caatctctgt
     4981 ggagctgacc catctatgtt tgcaactatt ttagcgaatg atatgggagg gtatccattg
     5041 gctatggaac ttgcacacaa taaagaggca ggaatgcttg caggattggt tgtgtcatcc
     5101 atgcttggat gtacattagt attttcaatt ccagtaggac taggtcttat agaatatgaa
     5161 gacagaccat tctttgcaaa agggttactg ataggcttga taacaatacc ttttggaggt
     5221 attataggag gattaatagc aggatttaac ttatcaatgg taattataaa tatgatacca
     5281 gtgataatat tggcagtaat attggcagta ggtttaaaat ttaattctac gcttatgata
     5341 aatgggtcat tagtttttgg aaaatttata tctatattga taacgatagg tttgggagca
     5401 gcagcatttc aagaaataac aggtatagta cttataaaag gcatgacacc tataagagaa
     5461 ggtattcaag taattggaag tattgcaatc gtgttattag gtacttttcc aatattacat
     5521 ttattagtta aagttcttaa taaaccactt acaatagtgg gtggaaaatt aggtatggat
     5581 gccactagtg cagcaggatt agtttttact ttagccaata gtattccagt atataaaatg
     5641 atgaaagata tgagtccaaa ggggaaagta gtaaatactg cttggctggt ttgtgctaca
     5701 gcagctttgg gggaccattt aggatttaca gcaggagtag ttccagagat gattacacct
     5761 gttgtaattg gaaaattggt tggaggagta ttggcaattg cacttgctat acttatgaca
     5821 aaagatttaa ccttggaaga agaacaaagt attaaaatga agaaagatgc agtgtagaat
     5881 aaacaatact taagtcaaat ttaaattatt ttaaagtagg agtttaaata actaaaaata
     5941 aattaaaaag acagacaatt aaaaaataat cgaataacta aaacttataa attaaataaa
     6001 atggcaatca aataactaaa aatgataagc taactgaaag tagttacata attaaaatat
     6061 aaagtatagt ttagacataa atggagctaa ataaataatt atttggctcc ttaattttaa
     6121 tttattacat caaggaacta aaaatcaatc atataagcta attatcagct atgactaaag
     6181 gtattccttt ttgagaaaac tctattatta tattatggtc tatactagca gaggtaataa
     6241 tagtatctat atgttctata ggagctactg atgcaaatga atttatacca aacttaggag
     6301 gttcgacaac tactatagat tttctagacc tttcaatcat tttttttcta acaagtgctt
     6361 cttctaagtg atagtcagag attccatttt caagagtgat acctccagct cctaaaatag
     6421 ctttatcaac attaaattgt tcaatagctg acagtgtaag cggaccagaa aaagaacctt
     6481 cagtttctct aagatagccc ccaagcacat atacttttat attagtattc ataagttcgt
     6541 ttgcaactaa aattgagttg gttattacag ttagattctt tttatctttc aaattttttg
     6601 aaacttcaag tacagtagtt ccagtatcca taaaaatagt atcatttgta tttataaact
     6661 cactacattt aatagcaatc tgttttttat cgtttgagtt tatttttttt cttttaacat
     6721 agttaagttc tacgctactt gctataactg gtatagcacc accataaact ctttttaaaa
     6781 gaccgatact ctccatatgg gctaaatctc ttcgtgcagt ttctattgat atatcaaaca
     6841 tttgaactat gtcatttatt ttaattatat tatctgattt gagaatactt aaaatcttct
     6901 catatctttc ctgtggtaac ataaaatatc ctccttggac tagtattatt ttagttttgg
     6961 cataacttac aattaagtca gagtttaggt atatttgtta atgtatgtat atttatcaaa
     7021 taatattata agtgttatgc aagataattt taccatattt aaaacttaca tttaagcaaa
     7081 aaagtaaaaa tagatgttta ataattcaaa ataactcaaa ttttataaaa caattgacta
     7141 aaatatgatt ttattatata atttattagt atagagatat ggagaagtac tcaagtggct
     7201 caagaggatc ccctgctaag ggattaggct gggtaaccgg tgcgagggtt cgaatccctc
     7261 cttctccgcc aattattaaa ggctattttt attaaagata gtctttttat aatgtcataa
     7321 atgattagat atgtataact aaagaaagga gggaatagat gacaacttta atttttttca
     7381 tagtggggat agcatctttg gtagttgctt ttgttattga tgggggacat gttggagcat
     7441 tatttgtatt ttcatcagca cttgttgtgt ttggtggaac tataggagct gttggagctt
     7501 cgactccatt ccctgtgttt aaaaagtcta taaagataat gttattagct tttaaaaata
     7561 aaaagcctaa taacatagaa aatataatct atttcaaaga aattgcaaca aaggctagga
     7621 aagaggggct tcttagtatt gacaaagaaa taagtgagaa tgatgaatta gaccctttta
     7681 ttagaaaagg gcttgaactt atggtagatg gagtggatgc agttactata agaaatatat
     7741 tggaatcaca gatacagata acatcaaaac gacataaaga aggaatagca atttttgatt
     7801 cagcaggtgg atatgcacct acactgggga taataggtac agttatggga ttagtacata
     7861 ttttaggtaa tctttcaagt aatccagatg cattaggacc tcaaatttct gttgcatttt
     7921 tggctacttt gtatggtgtt ggttctgcca atttaatatg gttaccaata gcatcaaaac
     7981 ttaaggctat aaatcaacaa gaagtaagtg aaagactttt ggtgatagaa gcaatattat
     8041 ccatacttga gggtgataat tctaatttaa tgacagaaaa attaaaaata tttttaaatt
     8101 caaaagaact aattgaactt gaagaaattg gtggtagagc tgaataatat aaaaggatgg
     8161 aatgtttatg ttgtatgatg aagatgaaaa agaagaaaac aatgagcgat ggcttttaac
     8221 ttattcagac ctcataacat tacttatgat tttctttgta ataatgtatt ctatgagtaa
     8281 tgttgatgcg gagaaatata aacaattatc tcaatctcta aattctgctt ttggtggttc
     8341 ttctggagtt attgaaggtg gtaatagcaa gatagaacca gtagttgaat caggaagtaa
     8401 tgatttagat actttacaaa atgctaagtt taaaaaagtt ggtgaagaaa tccagaagta
     8461 tttaaacgaa aatggtatgg ctaattcagt atcattaaga gttcaagata gaggattagt
     8521 aataagcctt aaagatacaa tactatttga tacagggaaa gcgattgtga aggataattc
     8581 tagagataag attattcaaa taggtaagat gttaaatgaa atgaatagtt acatgagagt
     8641 agaaggtcat acagacaata tgtctataaa aaattcagag tttaagtcaa attgggattt
     8701 atcagttatg agagcaacca atgttgttca acttcttatt gacaatgcag gtatagcacc
     8761 agataaatta tctgctgtag gatatggtga gtttagacca atagcagaaa attcatctga
     8821 agaaggtagg tcaaaaaata gaagagttga tatagtttta gttgatagta aatatgataa
     8881 tgtagaaaat gtttcaaagg ataaatagtt aaattagctg tctcaataca gaattaattc
     8941 taaaggagat ggctttttta tgcgaaaatc aaaaatagat acatagtaag ttaccttttt
     9001 gtaggaatat atggtatctt aaatatataa tatttaaata atgtataagc tcaattaaaa
     9061 aatttataga ggatggaaaa taaatgggaa ataaaaataa aaatactaaa tataaagata
     9121 aaacaagaga aaggtataat aaaaaagttg agaatttaaa taaagaaaaa ttaaataaag
     9181 ttaataagta atctaagaag gtagataaga ttgatgatat tattaataaa tctagtattg
     9241 tattatgtgt acttctagta gtgttttcat tgctttactt atttaaactg gttcaactta
     9301 atataataat gatactttta gtaattacag tagctattca acaaggattg gtgtcatttg
     9361 gtgcatttag aaataaaaat tatgtaaaag ctttattttc tatatttgta atagtatgtt
     9421 tcttaattgc ttttaaatat gttgtaaatc ctgtttaaat atatcaataa acgttctata
     9481 attaatataa atagtgaagt tacagtttta atgctattat aaattttata atttgaagga
     9541 gtagatttta tgaataggtg taagtttgca aaattaatta tcacatcaat gattgtttta
     9601 ttacttacag gatgtagttt aaagaaggaa gataattata agttagaccc agaaaatccg
     9661 acatcaatac aaatttggca ttattataat ggacagcaga aaatggcttt tgataagctt
     9721 gttaaggaat ttaatgacac tgtaggtgct gaaaaaggaa ttattgttga agcattcagt
     9781 caaggtaatg tagttgattt atcagagaaa gttttagatt ctgcaaataa aaaagttggt
     9841 tctgaagatg taccaaatat atttgcagca tactcagata ctgcatatga aatggataaa
     9901 ttagggttac tagtagatat ggataagtac ataagtaaag aagaattaaa tgaatacata
     9961 gacgaatatg tagaagaagg cagaataaat tctaaggatg agtttaaact tttcccaatt
    10021 gcaaaatcaa ctgaaatttt gatgttaaat aaaactgatt gggataaatt tgcaaaagct
    10081 acaaattcaa atataaatga tataaaaaca attgaaggat tagtagagac atcaaaaaaa
    10141 tattatgaat ggacagatag tttaacgaaa gagcctaatg atggtaaagc attttttgga
    10201 agagatgctt tagcaaatta tattattata ggaagtaaac aactaggagt agaaattttt
    10261 tctgttaaag atggtaaagt tacactaaat attgatgaaa aagttatgaa aaaactatgg
    10321 gataatttct atgtaccata tataaatgga tattttgctt cttatggtaa gtttcgttct
    10381 gatgatgcaa aaacaggtga tatattagct cttgtaggtt ctagttcagg agcagcatac
    10441 tttccagata gagtaatgct aaatgataat gagagttata atattgaaac atctgttttt
    10501 caagcaccac gttttaaagg agaaggaaaa tatgctgtac aacaaggtgc aggaatggct
    10561 gtaacaaaat ctactaaaga agaagaatat gcatcagtag aatttttaaa atggtttaca
    10621 gataaagaac ctaatataaa attctctatt gcttctggat acttaccagt aaagaaaaaa
    10681 acgaatgatt tggatgttat aaaagaagtt atgaagaaag ataatttgag tttatctgat
    10741 aaagttgaag gttcaatagc ctcatctatt gaacagatta acagttgtaa tttatatact
    10801 aataaagcat ttgatggtgg aactaaagct agacaaatac ttgaatattc aatgcttgat
    10861 aaagctaaag aagattcaaa taaaataaat gaattagtaa aaagtggaac ttctaagaag
    10921 gatgcagtag cacaatttaa taatgataaa aattttaaaa cttggtttga aagttttaat
    10981 gctgagcttg aaaatgctat aaaaaaatag atgaattggc agttagtgtg ttgacgagca
    11041 caaaggtata ggtggaatgg gtatggaaaa caatctaaga aaaaacaatt caataatgaa
    11101 aaagatgata tttcccctaa ttattgttat gctaattcaa acaagtttat tttgtgccac
    11161 aattttatgg ggaggaacta ttaagaaatt aaatgataat tcgtttgaca tcttaaatga
    11221 aagagtaatt aatcgtaaaa attatataca aaatgaaatg ttacaaagat ggtcaaatac
    11281 aagtgaaacg gaagctacaa ttaattcctc tgtaaaaaaa gtattaaatg aaaaaaatgc
    11341 cttaattgag gatgtaggca acaatgaaga aattaacaaa gatattataa aaagcatatc
    11401 aaatgattta atatatcttt tgagaaaaaa ttctgttaca ggagctttta taatcctaag
    11461 tgacaaaaat attaacgata agtcaataga tagtaaaggt gaaataagta ggacaggatt
    11521 gtatataagg gatttagacc ctaagtttaa tccaaatgat aattctgatt tgttaattga
    11581 gagaggttca tcagatatag caagaagttt tggaatatct atggacagtt attgggaacc
    11641 taagttttca tttaaaagtg aaaatgagca agatggagat gatttttttt ataaaccatt
    11701 tagagctgca attgataatc caaatgtatc aagttctaat ttaggatact ggggaagtaa
    11761 gtttttattg acaaatgcag gtgataggct tatgacatat acagttcccc ttatttatga
    11821 agatggaact gtatatggag tacttggaat agatttaagt gtaaattatt taagagaatt
    11881 acttccatac tcagagataa atgggaataa aaagggtaat tatattttag gtgtagatag
    11941 aaataatgat atggcctttg aaaatgtaat ttcaaatgga tactcattaa aagaaaaatt
    12001 ttgggatggt ttacattcta atttagagca gaaggaatcg tataaaaata tatataagat
    12061 taaaaataaa gataatgaat gtggtaatgg caacatttat gctagtgtac aatacttgga
    12121 tttatataac tccaatactc catttgaaaa tgaacgttgg gctcttttgg gtattattga
    12181 agaaaaaagt cttttgtcat tttcaaaaag ggtagagaaa ttagttataa tatctatagc
    12241 aatttcttta ttaataggtc taataggtat ttacattgca tcaagattat ttacaaaacc
    12301 aattgttctt ttagccaaca aagttagaaa cagtaatcca tcaaaaccag taaatcttga
    12361 taagataaat atatctgaaa tagatgaatt atcttcagct atagagttgc tcagtgcaaa
    12421 tgttgcagat tcttcgtcaa aattatcaca gattattgaa atggtagata tgccaattgg
    12481 ggcttttgag ttatacaaaa acgcagagaa tgttttttgt acaaaagaat tttttagcgt
    12541 gtttactact gaaatcaaat ttgaagaata tggatatatg gaaaaaaata agttcctgaa
    12601 aaaattaagg ggactagata aatatttgca agaaagttat aaaacagaaa atacatatat
    12661 atataaattt gaatctaaag ataaaaaact taagtggata agaatgaata tctttgaaga
    12721 tgagctaaag gttcttggtg ttgttgttga tgtcacaaaa gaatttattg agaaaaggaa
    12781 gatagaatat gaaagagatt ttgatatttt aacgaatctt ttaaatagga gagcttttca
    12841 ttcacagata caagaaaaat ttaagaataa agatgaactt aagatagcag catttattat
    12901 gtgggattta gataatctaa aatatattaa tgatacatat ggacatgatg ctggtgatga
    12961 atatattagg tgtgctgcag acatacttaa gaagtttata tcatggaatg gtatagtatc
    13021 tagactttca ggtgatgagt tttatgtatt tatatatgga tatgaaagta aagatagtat
    13081 aagagatatt attaattctg ttaagaaaca gatgaataat accttattaa aacttcatga
    13141 tggaacagaa tttaaaataa gggcatcagc aggaatttcc tggtaccctg atgattcaac
    13201 caactatgat gaattaatga gatacgcaga ttttgctatg tatcaaatta aaaatacaat
    13261 caagggtgaa gttagtgaat ttgatattaa tatatataat aaggattcat tcttattgag
    13321 taatagagaa gaattaaaca agttgattga tggtcaatta gttgagtatg catttcagcc
    13381 tattgtagat tgtaaaggta aagtatttgc atatgaagca cttatgagac ctaaaataaa
    13441 aacttttagt tcgccaatag atgtcattag attagctcgt tcacagtcta aattatatca
    13501 aattgagcgt attacatggt ttaaagcact taaatcattt aaaaaataca aagaaaaatt
    13561 tggcgattgc aaactgttct taaattctat tcctaataat aatttgtcaa aagtagattt
    13621 tgaggaaatt gaagatttat atggtgaata tttaaataga gtagtactag aaataacaga
    13681 gaatgataag attaatgaag attttattga gaaaaagaaa gcatatatag accattggaa
    13741 tatagaaatt gctttagatg attttgggac aggttacaac ggagatgctg tactattata
    13801 tataatgcca gactatgtaa agattgatat gtctattgta agaggtattg ataaagatga
    13861 aaacagacag aaaatattaa aaaatcttat ctcatattct aacgaaagaa atattaaagt
    13921 gatagcagaa ggtgtagaaa caaaaggtga aatggaaaca ctaattaatc ttggagtaga
    13981 ttatatgcaa ggctactata ttagtaaacc atcatttata cctgaagata taagcgacaa
    14041 aattaaaaaa gaactcaaga tatgtcatga aaataggaat gatgtggttt aatagaggaa
    14101 aaattaaatt tatttttatg aacttttagt ttataattaa aaagaaaata atataattat
    14161 aaatcaaggt ggtggagcta ataatcacaa caaatataga tataagaaag ttaaatgaaa
    14221 atcaattaga agcagttgag catatagatg ggccttgcat gatacttgca gggcctggtt
    14281 caggtaagac cagagtaatt acatatagaa tagccaatat ggttgtaaac aaaaacatag
    14341 caccaacaag aatcttggca ataagtttta ccaaggcatc ttcaatagaa atgaaaaata
    14401 gagctttgaa tttaagtgat gacataagac ttaataaggt aacctatggt acttttcact
    14461 ctgtattttt taaaattcta agatactttg aaagatacaa cttagacagt atatttgatg
    14521 aaaagtcaaa gcgaatgaca atcaaggcaa tcctaaaaag tttaaatata gagaatgctg
    14581 atgatgatga gaatatagga caagttataa atgaaatttc ctatgtaaaa aatgaactta
    14641 tggataaaaa tgaatttaat tcagaagtat taacaaaaga tgagttttta aaagtataca
    14701 acttatacga agaacaaaaa tctaaagtta ataaaataga ttttgatgat atgcttataa
    14761 gaacttatta tttacttttg aacaataagt ctgcactaga aatggttaga aatgtgtata
    14821 aatatatatt gatagatgaa tttcaagata taaataaagt acagtttgaa gttttaaagt
    14881 taatttgtag cccattaaac aatatatttg cagttggaga tgaagaccaa agtatatatg
    14941 gatttagagg tgctagacca gattttttgc ttgaatttga acaatacttt aataatacta
    15001 aaaaaataat attagatata aattacaggt ctaaaagtga gatagtgcat actgcaaata
    15061 gacttatcga caaaaataaa aatagatatg aaaaaatcat taagtgtagt cagggggatg
    15121 gaggaagtgt tacctatatt tctccgcatg attcagagga agaagcctta tatattgcca
    15181 gggaaataat tgacgaaata caaaaagatt atgtagagta ttcagatttt gcagtaattt
    15241 atagaactaa tatacagtca agagcattag tagatgtatt tatggatata aggattccat
    15301 ttgtagtaaa agactcagta attacaatat atgaccattg ggcaagtcaa gatatactag
    15361 cctatttaag aataggtatt aatccaaaat caaataaaga ttggttgaga attataaaca
    15421 agccttttag atatatatcc aaagacagta taaatatggt taaggatgaa aaagacttta
    15481 taacagcatt aataaataag tgtaatcttc atcctaaaca agttaaaaca ataaatgatt
    15541 tggaaataga tttaagttat ttaaatactt taaatcctaa aaatgcaata tcttatatta
    15601 gaactagtct tgattatgat agatatgttt tagattattg tagcaacaga aagataaaga
    15661 ctaatggact tgtagagata ttaaatgaac ttgagagttc agcaactaat tttaatactg
    15721 ttacagagtt tttagagcat attgatagag taaagactga gttaacggaa aataacaaga
    15781 ataagcagac tgagggtgtc atatttacaa ctatgcatag tgcaaaagga ttagaattta
    15841 gaaatgttta tattataggt gtaaatgaag gaacaatacc tcatgaaaaa tcatatgata
    15901 tttgtaagga agagaaaaaa gaagaacaat tagaagaaga aagaagactt atgtatgttg
    15961 ctattacaag agctgaagaa aagttatgta taagctctac tttaaataag tatggtaaga
    16021 aagttgataa atcattattt attaatgata taaaatctcc aacgaaaaaa gaaattgaca
    16081 gtattggtgt aggggataag gtataccata aaaaatttca tgaaggagaa ataattaaaa
    16141 aagatggaat tatgtttaca ataaagttta aagatagaga gcgaatcttg gatttgaaaa
    16201 cttgtttatt gaagaatata atttatacta tttaatttag aatctaaatt tagataggaa
    16261 tataatgtat tatttattat gtgcaaggca aatagatatg tgtaaaatgt attatttaac
    16321 ttattagaaa aagagataag ggtttaatat attatttata gttgtttaga aaaagcaaca
    16381 atgtggaaaa tattatttag tattattaaa gaaaagcaac aatgtgaaag atattattta
    16441 gtgtaattta agaaaaagca acaatgtgaa agatattatt tagtattatt aaagaaagag
    16501 caacagtgtg aaagatacta tttagtatta ttaaagaaaa agcaacagtg tgaaagatat
    16561 tatttagtgt aatttaagaa aaagcaacaa tgtgaaagat attatttagt attattaaag
    16621 aaagggcaac agtgtgaaag atactattta gtattattaa agaaaaagca acagtgtgaa
    16681 agatattatt tagtgtaatt taagaaaagc aacaatgtga aagatattat ttagtattgt
    16741 ttaagaaaaa gcaacaatgt gaaagatatt atttagtatt atttaagaaa tatctaaaaa
    16801 tatgaaatat actatttata attacacaaa taatatcaat aaatataaac agtagtattt
    16861 agataattta caagtaaaaa ttattaaaaa aataaaaaac ttattgacaa aaatattata
    16921 aggaagtaca attgtattaa caatttaagg aaacaaatcc aatgatagag aaagtagcat
    16981 atatggattt acagcgagtt aggggttggt gtaagcctag caatgaagtc tatgtgaaga
    17041 gagctctgga ggagattatc agaaacttta gtatgataat tggtttaccc gccttaaggg
    17101 attgagagga taagtactta cttatcaaaa agagtggtaa cgcggatata attcgtctct
    17161 tagctgtaaa gctaagggac ttttttgatt taaaaataaa agtatccata cagcttaaat
    17221 gctaacagtc acaaattttg aaagtaatat aagtatttat caataagagt ggtaacgcgg
    17281 taaagtcgtc tcttagtcat taaagactga gagacttttt ttgtaactcg aaacatttaa
    17341 gtattaaaat ctgagggaaa ttataattaa aagagagggg tagttaaaat gaaagtattt
    17401 aaaaaattat taagtttagg attagtatta ggattgacat tatcgctagt aggatgtagt
    17461 ggtggaggag aaaaaacaaa gttagaacaa ataaaagaca atggaaaatt agttgttggt
    17521 actagtgcag aatttcctcc atttgaattt cataaggtag tagatggaaa agattcaata
    17581 aaaggattcg atataatgtt ggctgaagaa tttgcaaaag agcttggtgt aaaggttgaa
    17641 attaaggata tgtcatttga tggattaata ggtgctttaa atgcagacca agttgatatt
    17701 gttcttgcag gtatgtctcc aacaccagag agagaaaaga gtgttgattt ttcagaatta
    17761 tattatttaa gtagaaatgc agttatagtt aaagatgcag atatagacaa agtaaaaact
    17821 gaagatgatt taaagaaact tagagttgga gttcaagcag gaagtatcca agaagaatat
    17881 gtagttaaca ctttaaaaat gacaactaca aaatctttga aagcaatacc tgatttaatt
    17941 acagaattaa aaaatggaaa catagatgca gttgttacta atgaagcagt ttctttaata
    18001 aatgttaaaa aatatgatgg aataaaaatg gcaaatactg aagttgggaa agatgtaaca
    18061 gaaggtatgg cagcagcaat caaaaaatct gacaataaca aagattttat agaattatta
    18121 aacaaaaaga taaaagaatt acaagatggc aagaagatag aagaattttt aaatgaagca
    18181 tctactgaag ctgcttcaaa ttagtaagaa agttatgtgg gagatgaaat tatgagttta
    18241 gattttagtt ttttaagtag atttgggact tcttttttgg aaggaacagg tgtgacagta
    18301 tcaatttctc ttgtggcatt atgctttgga tttataatag gtataattat ctgtatggca
    18361 aaaatatcaa agagcaaagt attaagagca atatcatcaa tctatattga ggttttaaga
    18421 gggacaccat tattagtaca gatatatata gtatggtttg gtttacctca attaggaata
    18481 agattcccta tgttgtttgg tataccaagt gaatttatag ctagtgcatt tgcattatca
    18541 gtaaactctg gtgcatacgt ggcagagata cttagatcag gtatacaatc agttgataat
    18601 ggacaaatgg aagcaagtag atcattagga ttaaattatt ggtcaactat gagatatata
    18661 ataatacctc aagctataaa aaatatatta ccttcattag caaatgagtt tattacactt
    18721 gtaaaagaat cttcaataat atctgtaata ggagttgttg agataatgcg tactgctgat
    18781 atagtaaaaa atgcagcatt tagagcatta gagccattaa tagtagcagc agcaatttac
    18841 tttgttataa catttactct atcaagatta gttggattat tagaaaagaa actatctgtg
    18901 tctaactaga tgagagcatg tttagcattt agggaaaaca tagaatagta aaaaggaaag
    18961 tgataatatg attacaataa aaaatttaag taaatctttt ggagatttaa atgtattaaa
    19021 aaatatagac ttagaaatcg ctaaaggcga aataatggtt atagtaggtc caagtggttc
    19081 tggtaaaagt acacttctta gatgtatgaa tttactagaa atacctacag gtggagaaat
    19141 aatatttgaa ggaaaaaatt tggtcgataa aaaaactaat attgatgagg ttagacaaaa
    19201 tataggtatg gtgtttcaga actttaattt attcccacat aagactattt tagataatat
    19261 aacattagca ccaattaaat taaaaaagat gactaaggaa gaagcagaaa aaaaagcaga
    19321 aattttgttg agtagagttg gtcttttaga taaaaaagat tcatatccat cacaattatc
    19381 tggaggacaa aagcaaagaa tagcaatagc tagagcactt gctatggaac cagatatgat
    19441 gttgtttgat gagccaacat ctgctcttga cccagaaatg gtaaacgaag ttttggatgt
    19501 tataaaagaa cttgctaaag aaggtatgac aatggcaatt gtaactcatg agatgggatt
    19561 tgctaaggaa gttgcagata gagttatttt tatagatgga ggaagtatac ttgaagataa
    19621 tactccagaa gaagttttcg gaaatccaaa acatgaaaga acaaaagcat ttttagcaaa
    19681 agtgctataa ttatataaaa aggatgttat tgatattaga aattcaatac atcctttttt
    19741 agtgatggtt aatattaatt ttaaaattaa agttatatta atattaactt tatttttacg
    19801 attttttgca gaaaaaaaaa gagctcgggg taagctcttt ttttccaccg aattaatttc
    19861 cgttactaat ccgattttta ataaatataa aatatatcct tatgaaaggg tattgggaat
    19921 agatatatta actaactagg gttaccaggg ggataacctt aatttattat aacaaaatgc
    19981 gacacaaaaa tcaatatttt ttttgaaata aattataaaa gttattttta taacaagtaa
    20041 gcttataaat ttaactttta tagtaaaaat aagtatatgt ataa
//
LOCUS       DAEMHZ010000062        20043 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000062 DAEMHZ010000000
VERSION     DAEMHZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.062"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(593..1594)
                     /gene="trpS"
                     /locus_tag="KPK72_002152"
     CDS             complement(593..1594)
                     /gene="trpS"
                     /locus_tag="KPK72_002152"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG2489365.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(1617..2315)
                     /locus_tag="KPK72_002153"
     CDS             complement(1617..2315)
                     /locus_tag="KPK72_002153"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG2489366.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(2358..2598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2689..4092)
                     /locus_tag="KPK72_002154"
     CDS             complement(2689..4092)
                     /locus_tag="KPK72_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2489367.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     gene            4589..5665
                     /locus_tag="KPK72_002155"
     CDS             4589..5665
                     /locus_tag="KPK72_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2489368.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(5782..6624)
                     /locus_tag="KPK72_002156"
     CDS             complement(5782..6624)
                     /locus_tag="KPK72_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2489369.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKNVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            6808..7380
                     /locus_tag="KPK72_002157"
     CDS             6808..7380
                     /locus_tag="KPK72_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489370.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(7543..7815)
                     /locus_tag="KPK72_002158"
     CDS             complement(7543..7815)
                     /locus_tag="KPK72_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489371.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(7929..8801)
                     /locus_tag="KPK72_002159"
     CDS             complement(7929..8801)
                     /locus_tag="KPK72_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2489372.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(9097..12204)
                     /locus_tag="KPK72_002160"
     CDS             complement(9097..12204)
                     /locus_tag="KPK72_002160"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG2489373.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(12297..12529)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12704..13231)
                     /locus_tag="KPK72_002161"
     CDS             complement(12704..13231)
                     /locus_tag="KPK72_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG2489374.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(13266..14051)
                     /locus_tag="KPK72_002162"
     CDS             complement(13266..14051)
                     /locus_tag="KPK72_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG2489375.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(14061..14318)
                     /locus_tag="KPK72_002163"
     CDS             complement(14061..14318)
                     /locus_tag="KPK72_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG2489376.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(14337..14795)
                     /locus_tag="KPK72_002164"
     CDS             complement(14337..14795)
                     /locus_tag="KPK72_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG2489377.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(14835..15539)
                     /locus_tag="KPK72_002165"
     CDS             complement(14835..15539)
                     /locus_tag="KPK72_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG2489378.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(15549..16184)
                     /locus_tag="KPK72_002166"
     CDS             complement(15549..16184)
                     /locus_tag="KPK72_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489379.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            16565..17605
                     /locus_tag="KPK72_002167"
     CDS             16565..17605
                     /locus_tag="KPK72_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2489380.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPSGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(18068..19423)
                     /locus_tag="KPK72_002168"
     CDS             complement(18068..19423)
                     /locus_tag="KPK72_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG2489381.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(19449..19718)
                     /locus_tag="KPK72_002169"
     CDS             complement(19449..19718)
                     /locus_tag="KPK72_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2489382.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         6469 a         3466 c         2270 g         7838 t
ORIGIN      
        1 aaaaattaac tttataaggt attttttatt taaatattat cttcttctaa tatattcttt
       61 tttagaacta tattgtatta aaaagataat tatctgtatc atatcaccta tatataaaca
      121 taaattcatt taattatata aatttaaaaa atattttttt gatatattat tgacaatttt
      181 cattataaga gttaaaatta actcataaat tacatattgg gtataaaata caatgtatgt
      241 tagaagttgt ttcagaaggc tatggttttc tgattttttt atgctcagta gtgatatgca
      301 aaaaagacaa agttgacatc agttaacttt tacctttatt ctactttccc caaagtaaga
      361 ataatctaac ccctttgaga tgagcaaaag ctcatctttt tttattatca aaagatgctg
      421 tctcaaaaca gagataatcc tctttttcat tgttgaaatg attaaaatat attttatttc
      481 atacaaaaaa gagtgcctca tgaacaaaag ttcactttga gactctctct ttcatatcat
      541 ataccttttt aataacttta aaatttattt atcaagtaaa gatagctaat aattagtatc
      601 ttctctctat aaatccaact tttttataaa cttttctaag agtttttcta gcttgttttt
      661 cggctttttc tgctcccata ctatatattt tttctaaata atctttatta ttaagaaggt
      721 caacatattt ttctcttata ggtcttaaag cttctgatac tacttcagcc acatcttctt
      781 taaatatgcc ataacccttt ccttcataca tgtttacaat ctcttctaca ctctttttac
      841 ttaaattaga atatatatca attagatttt taatacctgg ttgttcatca ttgtatttta
      901 caacacctaa agaatcagta acacttcttt ttatctttct ctttatagaa tctgaatcat
      961 ctgctaatag tataaatgca ttttcatttg aatcggattt actcattttt ttagttggct
     1021 cttgcaaact catcactctt gctccacctt ttgggtagta tccttctgga actacaaaag
     1081 taggtgaaaa tctattatta aatctatttg ctaaatcacg agctaattct aaatgttgtt
     1141 tttggtcatc tccaactgga actaggtcag tttgatacaa caatatgtca gcagccataa
     1201 gaactggata tgtgaataaa cctgcattta gatttgcttc actcttttgt gatttatctt
     1261 tgaattgagt cattctactt aattcaccca tataagtcat agtattcaat acccacataa
     1321 gttcaacatg ctctttgaca tgtgattgta taaatatagt atttttttct gggtctaatc
     1381 cacaagctat atattgagca agtaattcta tagtattggc tcttaaaact ttagggtctt
     1441 gaggtactgt tattgcatgc aaatcaacta tactataata acaatcataa ttgtcttgaa
     1501 gttcagtcca gttttttata gctcctaaat aatttcctaa agtcattttg cctgatggct
     1561 gtgcaccact taatataact tttttttcac tcattttttt gtacctcctt atatatttat
     1621 attgatttta acatatttaa aactatgtct ttagagttat tggcagcatc atgtacaaat
     1681 acatcataag taacatcagc acttccatct gccttatcag acattgctct tattattaca
     1741 aatggagtat tatttaaata gcatacatga gctatagctc caccttccat ttctccacaa
     1801 tatccaccaa aatcatttac taattcttct tttaattctt tagaatttat aaatttatct
     1861 cctgtaacta ctcttccttt taatacttta tgagtcttta cttcttccac agaagatttg
     1921 taagcagcat ctataagtct ttcatctgct ttaaatacag aagtcttcat tcttggtata
     1981 actcccttag ggtctccaaa tgctgtagtg tctacatcat actgtatagc atcagttgat
     2041 attactatat cattaacatc aagtttttcg tttaaagctc ctgcaacacc tgtattaaca
     2101 atagcatcaa ccttaaattc gcttattagt atctgggcac aaagagcaga gttaacctta
     2161 cctatgccac actttactaa cactacattc ttaccttcca aaattccctt ataaaattct
     2221 aagctagctt tttttatagt ctctctaata tccattaaat caactaaaat actaacttct
     2281 tcatccattg cacctataat tcctattacg ttcatttttc ctcctaaatt ttctatatta
     2341 aaaaatccgt cctaaatcaa taggacggat atattttccg tggtaccacc taaatttata
     2401 tacactcaaa attgaatata tatatcttaa tatatgctat aacgtgcata cacgatatca
     2461 aatactctgt aatcttaaat tacatttcat tttactcctc aaagacccat tcaataaata
     2521 ctttttatta agttctcagc atccttaatt ctctgtgcaa aaatatattt atttactatt
     2581 tcttatctca ggatttaatt tattaatttc tttattttat aatattcctt acactataaa
     2641 ttgtcaacta tatattataa tatccaaaag aaatttttaa aatacatttt atttagctgt
     2701 tttcttttca tatctattcc ataaccatag accagataat ataactagaa gacctccacc
     2761 aatcatctct atctcatata taacattttc agcaaatgta ggcataactg gtgctgcaga
     2821 taataatata cccactacag ttacaactaa aaccattttt gctaaaccta ggcaagtact
     2881 tgttgatttt gccatagtat aaggtctaat ttcatctggt ctctcttttc ttagtttaat
     2941 ataagaagca tataacaata catatgggaa taatgcagtt aatgctgtca tagttacaag
     3001 aacattgtat atagcatcaa cagaaggcat taatgtagtc accaatagta ttatagtaac
     3061 tattattgct tgaagtataa ctgctttaga aggtatcttg tgttcattta cctctactaa
     3121 actatctgga aatattcctt ttttaacact accaaataac atttttattg gtgatgctat
     3181 gtaaagaact attgctccta atactgaaaa agctatacca aaagctatta attgaaccaa
     3241 ccaagaacca attcctaaag acgcacaaac agcagaaagt gcatctaata cacctttaga
     3301 agcagtaatt tggtctggtg gtattatcat tgttattgca attgaaccta atacatataa
     3361 tgaagctact attccagctg atattaatat tgccttaggg aagttctttt caggcttatc
     3421 tatttctgtt atgaagttag cagcagtctc tgctcctgct aaaccaaaca ttatagctga
     3481 tatagcaaca aatgcatcca tattaagttt tggagtcatt gaagctattg tgtatgtaga
     3541 agctatatca tgttttttca acacaaatac tgctacaaat gaaaatatta taagtatagc
     3601 acttggtatt gttgaaccta atgcacctgt attagtaaat atttttgtaa aacttattcc
     3661 tttagttgct ataagagata atgcccagaa tattattaaa gataatataa gtacaaacat
     3721 cttattatca gcaagggctg gatttcctat tgtataagaa acatttactg ctaagaaagt
     3781 taaaaatgaa gaataccaaa atattttagc tgtccagttt aaccaagaaa ctaaaaatcc
     3841 atacttttct ccaaaggctt ccttaaccca atcgtaaaga cctccatcac ctttttgata
     3901 tgtagaagct aattcagcac aaattaaagc tcctggtaca aagtatataa atgagaataa
     3961 taaccacata ggtattgcac ctaatcctaa accaaagctt gatgctgtag atttagctat
     4021 ccatcttata ccatataaag ctgatacatt cataaatacc aaactccata ggcctatttt
     4081 tttcttttcc atcctaattt aatttcccct ctctattaat tattttaaat ttaatttcaa
     4141 aattaataaa atagattcat tattattaaa ctgatatttt tcaaaatatt tgcatattca
     4201 aaaacagttt taataataaa ccttaaatac tttgaaatca aatcttatta aaaatgataa
     4261 atttaaaagt tttaccactt taattctaat gatactaaat ttttaaaatt gtttcaatat
     4321 tattttatta taaaatttta tttattattt ttcattagta tataaaaaga taaaatacat
     4381 aactttatag acttaattta ttacttttat agaattttca caataaaaag tcattttact
     4441 tccgtattta atgcaaaagt agagattaaa cttaatataa aaaatatata acaaattttt
     4501 tttatatttc tagaagaaaa gtatactctt ttttgtttaa taatgctaaa attgatttgt
     4561 attgaatttt gattaggagg aaattattat gaaagctcaa agattaaatg ctgtacttga
     4621 acaaatgaaa aaagatgata tttctcaaat gttagtaagt gaccctactt ctatatttta
     4681 tcttacagga gtactaatcc atccaggcga aagactatta gcattatact taaatttaaa
     4741 tggtaataat aaattattta taaatgaatt attcccagtt tcagaagatt taggtgttga
     4801 aatggtttgg tttaatgata ctcaaaatcc agtagaaata ataactgaac atatagacaa
     4861 aaatgctact atgggtgttg ataaaaactg gccagctaga ttcttattaa acttaataga
     4921 attaggtgga ggaagtaagt ttgtaaattc atcttatata atagatactt taagaatgtg
     4981 taaagatgaa gaagaaaaag agttaatgag aatagcttca aaattaaatg acaaggctat
     5041 ggaacaactt aaagctactg tttctggtga acttacagaa aaacaacttg ttggtaaatt
     5101 atctaaaata tatgaagacc taggtactga tggattttca tttgacccta ttataggttt
     5161 tggacctaat ggagctaatc cacatggtga acctggaaat gcacttgtga aaccaggaga
     5221 tgcaataatc ttagatatag gttgtataaa agataactat tgtgctgata tgacaagaac
     5281 tgtattctat aaagaaatac ctgaaaaagg tagagaaata tttgaaatag ttcttgaagc
     5341 taataagaga gctgaagcta ttgtaaaacc aggtgttaga ttctgtgata tagatgctgc
     5401 tgctcgtgac tacataactg aaaagggata tggacagtac ttcactcata gaactggtca
     5461 ctctatagga cttgaagttc atgataaagg agatgtatct tctataaata cagatactgt
     5521 acaaccagga atgatattct ctatagagcc aggtatatat ttaccaggtg agttcggagt
     5581 tagaatagaa gatttagtac ttgttactga agatggttgt gagatattaa acaagcatga
     5641 caaagaaata tgtgtagttg gttaattcta aaatcaattt gataaaatta aaaagccata
     5701 taaaaatgtt ttaaacattt ttatatggct ttaatttctc aatctattat ttatttcaat
     5761 ccattattta tattgcagta tttatattgc agttattaag attgcaccct ttgaagtata
     5821 tgtccccatc actgaaccaa tttcacagat tacaacatct ttgtatttta cattcttttc
     5881 cataacttcc cttacctttt gagccttttc tgggcaatta gcatgtccta taaataaaac
     5941 cttttcttct ttatctttaa tttttgactc tactaaagta attagctttt ttaaactgtt
     6001 tccttcacct cttgccttat ctataggagt tacaacgcca tctgcaactt gtattatcgc
     6061 cttaaaattt aaggtattta taatttttcc tgctattgga tttattctac cacccttaat
     6121 agcattttct aaagtttcaa gtgttccaaa aaatggagga ttatctttat atttatttat
     6181 tattgaaaca atctcttcaa tttcttttcc ttcttttatt aatattgctg cttttaacac
     6241 taattgtcct attccaattg acccactaag tgaatctata acctcaactt tcttgttatt
     6301 tccttccttt tcaaacatat cttttgctaa aagtgcacta ttataagtcc cagataatcc
     6361 tgaacttata ttaatcacta atacactttc ttcctgacaa tggtatgctt ctaaaaattt
     6421 atcaggagaa ggacatgatg tttttggtag tactttactc tcactcattt ttctatagaa
     6481 agtttcatta tctatttcct ttccagatat ataactttct tctccaaaag atacatttaa
     6541 accaacaact tctatgttgt atttttctat tatatcatca ggtaagtcac atgcactgtc
     6601 cactatcaat tttattttat ccatattaac ctcactataa gatattattt tatttattca
     6661 gtcggtcgta cgaccgattt aattattatt ttatatattg aatgaaaaaa agtcaataga
     6721 tatagattct cgctttattg acaataaata tccataagct tataatgtta ttaatttgtt
     6781 ataaatatac atggaaaggt aggttttatg aaaacaaata atatgaaaca ggctatttta
     6841 tcatctgcaa ccaatttaat agccaaaaat ggagttcaaa atactagcct tgctgatatt
     6901 gcaaaagatg taaatataag taaaggtact ttgtattatc actacgcatc taaagatgat
     6961 attatatatg atattgcgga tactcattta gaagttataa ctagtgctat actaaactgt
     7021 gtaaaaaatg ttaaatcaaa aaattctcaa atagaaatgg ttaacttaat cttagagaaa
     7081 atatctacta tagaaagtcg tggtagggta catatgtatc ttatttgtga ggcaattact
     7141 tcaaataatg atttaaagga aagaataaga ttaaaatata ttgaatggag aactactctt
     7201 aaatctgaaa ttgttgaatc actagaaaaa tataatagta gcgaaaatga acaggatgct
     7261 gaatcatttt cttttttact catgtcaata gttgatggtt tagttgtcca aagtttatta
     7321 aaaacagaaa aaatacctta tgaaaatata gcttcttttt tagttaatca ttggatttaa
     7381 acttaatact tagataatta ataaaagtga actatctaag ttatctactt taacagttca
     7441 ctttcttatt tattctcaat aatttttact taaaacattc aatttttatt taaaccttca
     7501 tggaatcaca aaataaaaat ctcttataaa tttaaaatgt ttttatttat gttcatgtgt
     7561 atgcccatct gaatgttcat gaccatgtgt atgtgtatgc tcatggctat gctcatgagt
     7621 acctgaaaaa ccatcatcat gattatggtc gtgacctaca ccatttacat gctcatgttc
     7681 atgcccatga ctgtgagaat gttgatgtga atgttcatgg ttatctcctc cacagcatcc
     7741 ttctcctcca gtcccacaac acccacttgt atgtggatta tgatgatttc ttactacaaa
     7801 tctctctctt aacataattc ttatacctcc tagtatatta ttgagtactt catgtacacc
     7861 attaactaca gtatacatct aactaggtat aatctcaata catatggcat tttttaacat
     7921 atataatctt aaagaaattt atccttaaaa cctccacctt ttacttcttc aacgttattg
     7981 acagaaataa aagcttcact atccatatca taaattaaat ctttaaattt acctatttca
     8041 tttgcagata ttatgcaata aatcatcttt ttcttatttt gtgtatatgc accttctgcc
     8101 tccaaaaaag tgactcctct acccatttta ttcattatca ctttggatat ttcatcatat
     8161 ttatttgata ttaaaagtat agattttttt ctatcaaagc aatcttttcc tatatccata
     8221 gtaaaagaaa ttatatacat tgtaattaaa gtgtacatca ccaatttagc tccaaataaa
     8281 aaagctcctg cacatactac aaaaaaatta ataaataaaa atgtattttt cataggtata
     8341 tcatactttc ttttcattat ggctgctatt atatcaagtc ctccagcagt agctcttgct
     8401 ttaaaagtta accccatacc tataccagtt aatgcaccac cataaataca ctgtagcata
     8461 gtatcatcta ttgggaaata tttagcaata tcttgtgtta gccccagtgc tagagaaaat
     8521 agtaacatat tgacaaaact tattagcaaa aatttattat caaaatactt tctagctata
     8581 ataaatattg gtatgttcat caagaaactt gatattccct gattaatacc aaataatttg
     8641 tataatataa cacaaattcc agctactcct ccacttaata acttggctgg tgctagatat
     8701 aaatttattc ctaaggttga tagaatcata cccaatataa taagacaata ttgtaaaaat
     8761 atattattat caatacttag ctttttactt tcaccataca tcaaaacgca cccctttgca
     8821 taataaataa accaccctgc ataatacacg ataaatttac aaataaattt atcagaagta
     8881 gtttcctaaa aatattattc atcctcactt aaacgtttat tcattaatat tctatcacat
     8941 gacataaata atttatatga tttatataga tttatttatt gtaaattttg gaatattaaa
     9001 acattttatt gaatagataa ttatattaca taaaaaatcc ctatagaaat ctataaacta
     9061 atttctatag ggattttggt taactaacta ttatttttat ttttttataa cttttatacc
     9121 tgtcatatgg tcatttaagt tttgttcctc taaagattta tcatctactc tgtttatttc
     9181 taaagctaaa gtttcagact tgatatattc attaaaatgg tcaacagcct tagcaatttc
     9241 atcatcacca cagtattcta ttactatatt atctaatact tcaaagttat tagattttct
     9301 taattgctgt actttagata taaattctct agcataacct tcattaatca actcttcagt
     9361 tagcttagta tctagtataa caaaaagatt gttttcaaca gatacattaa atccttcctt
     9421 agcagttata cctattaata ctaaatcctt attaaattca aaagcttcac catctaaatc
     9481 aactgttaaa gtctcacctt tttcaagttt attagcagtt tcatgagcat ttaatttact
     9541 taatgctcca acgaataaat tcatcttaga acctaataat ggccctgctt ccttaaagtt
     9601 tggttttaaa ctaaagttca tatactcatc taaatcctta gcaaatatta cctctttaac
     9661 attaagctct tcttttatta aatcaactac atcacttatt gtatcttcaa acttaccatc
     9721 tactaacact ttttgtaatg gttgacgaac ttttattctt tctacttctc ttgaagctct
     9781 acctaaagta actaagtttt tagctaaatc cattttttct tctaatttag tatctactaa
     9841 ctcttcatta catttaggat attcagctaa atgaacagat acttctcctg ttaagtttct
     9901 atacatttct tctgacatat atggtgcaaa tggagctata gctctacaaa gaccatgtaa
     9961 caattcataa gttgtattgt acactgcttt tttatcttct gttaattcag ttgcccagaa
    10021 tcttcttctt gaacgtctta tataccagtt agataaatca tcatttataa aatcttgtat
    10081 cattcttaaa gttttattta actcaaatat agctaaattt tcttctacat ctttcattaa
    10141 attattgaac ttagataata tccatctatc aagttctggt ctatctttat attctacaaa
    10201 aaattcagtt ggatttatat catctgtgtt tgcatataaa gtaaagaagt tatatacatt
    10261 cttcatagta cctataaatt tactttggat ttcttttaat ccatccatat caaatttagt
    10321 tggagtccat ggtggagaaa catatagtaa ataccatctt aatgcatctg ctccatattg
    10381 gtcaaataat tccattggat ttactgtatt tcctcttgat ttactcattt tcttaccatc
    10441 tttatcaagt acaaggtctg gaactaaaac actcttatat ggtgcttttc ctaatacaaa
    10501 tgttgatact gcaagtaatg agtagaacca acctctagtc tggtctatac cttctgatat
    10561 atagtcagct gggaacaatt ggtcaaagtt ttccttattt tcaaatggat agtggtgttg
    10621 agcaaatggc atagccccac tatcaaacca acagtctata acctctgtta ctcttgtcat
    10681 tggtttccca catttttcac attttaagtg tatatcatct acatatggtc tatgaagttc
    10741 aactgtttct ggatttacat cttctatagc tttttcagct aattcagctc ttgaaccaac
    10801 tgaatctgta tgaccacatt cacatctcca tataggaagt ggagttcccc aatatctagt
    10861 tcttgatatt gcccagtcat ttagattttc taaccagttt ccaaatctct tctctcctac
    10921 aaaacttggg aaccatttta caccattatt attttctatt aatttatctt taagtttagt
    10981 catctctata taccagcttg gttttgcata gtatactaat ggagtttgac atctccagca
    11041 gtgtggataa ttatgaacta ctttttcttt gctatataat ttattttcag aagctagcca
    11101 ttttattatt tcaacatcta gcccatcttc cattacaaat ctaccttccc aaggagtaga
    11161 tgtaaattta ccagtttcat ctactggttg aataactggt aaatcatatc ttttacctgt
    11221 gttgtagtca tcttcaccaa atgctggtgc tgtatgaact ataccagttc catcttcagt
    11281 agttacataa tctcctactg taataaaaaa tgcttttttg tcaacttcta caaaaggcat
    11341 taattgttca tattctaaac cttctaggtc tttacctttc atttcttcta aaacttcata
    11401 atcttcacct agtactttat cagctaaagg tttagataca taatatacct catcaccttt
    11461 tttaactttt aaataatcta catcagcatt tactgttaat gaaacatttg atggtaaagt
    11521 ccatggagtt gttgtccatg ctaagaagta ttcatcagca tcttttttct taaatttagc
    11581 tataactgta ttagttttta cttctttata tccttgtgca acctcatgac tagcaagacc
    11641 agttccacat cttgggcaat aaggaagtat tttatgccct tcatatatat atccttcttt
    11701 gttgaatttg tttagtatcc accatacaga ctctatatag ttattatcta aagttatata
    11761 tggattatct aaatcaactt cataagccat tctctcagtc attattctcc attgtttttc
    11821 aaatgagaaa actgactctc tacatttttg gttaaattca gctattccat attcttctat
    11881 ttgttgctta ctagtaagtc ctaactcttt ctctacttgt atttcaactg gtaatccatg
    11941 tgtatcccaa ccagcttttc tctttacttg ataaccagac attgttttat atctacaaac
    12001 tgaatcttta agcgttctag ataaaacatg gtgtactcca ggatttccat tagctgttgg
    12061 aggtccttca tagaatacga aacttgggtc atctttccct ttttctaaag ttttttcaag
    12121 tatatttatg tctttccaat aatcaaaaac ttgtgcttct gcttgtttta cagaactgtc
    12181 taccaatggc ttaaatttcg ccatgtaaat caccctttcc tactaaatat tataattcca
    12241 aaatttttta ttctttcaca taaatttaaa tactaaaaaa ctcgccccct ttaaaccaaa
    12301 gggacgagtt ataaatcgcg ttaccaccct aattctatat gtaaactaaa tactagctac
    12361 atataacact ctaatagtat aacggtacta accggcacag cttagtagaa tttaatcgtt
    12421 cagcctgcag ctcaagagtg atttttatct ttataaattc attggttctc agcataccca
    12481 attctctgta aaattatcta aagataaatg tctctatcaa cgcttttaaa aatttatctg
    12541 tgtttatatg aaattctata acaaataatt tgaatagtca atatattttt gaaaaatata
    12601 ataatttcta tttttcttac atttttatcc tatttttaat ctttgttata gatttctcaa
    12661 actcacacaa ttattctaca taaaaaataa acaaattaca agcttattct aaagttgtag
    12721 cagcttcatc attactataa ttatacactg ttgttccttc aaatgcattt acacattttt
    12781 catctacatc ataaaaaatc tcatcaacac ttcttatttc atcttctaac agtgatttaa
    12841 atttatttct aaatatctta aattctttga ctaaagcatc atactctttt cttatctcta
    12901 taacacggtt attagcttgt tctataattt gtctagcttt taaatcagct tcttcaacaa
    12961 ttatcttagc ctttttatta gccgcgctac aagtgtcttc tgctgcactt tgagcagtta
    13021 ttaaagtagc ttttagtgtt tcttcaatat tttcatactt gtttacttgg tcttgatata
    13081 accctaattt ttctttaaga gctgtatttt ccctacatag agattcataa tcttccttga
    13141 tgatatctaa aaattcatca acctcttcat ctctataccc tcttaaacct tttttaaact
    13201 ctttattttc tatctcaatt ggagttagca ttacatttcc tcccagctat aatttaaatt
    13261 agtccttata caattaatct tgcttgaact tttatttttc cttttttagt aatatctcct
    13321 atttgtgtta ccttagctct tcctttgcct ctaatagaaa tcaaatcatc attttttact
    13381 attttagaag ttgaagttat cttttcataa ttgacatgga ctttttctcc atttatgtat
    13441 tttgcactat cttgtctaga tatattgtaa ataccactta tcacacaatc taacctatct
    13501 gaagatactg taaatgatac ttctttatat tttgaagaac tattaacaat attttctctg
    13561 gaaatttcct ttacaattac attatttctt gaaacctttt ctaagtttac aattataaaa
    13621 tcgcaaatat cactacttac aactacttga caaaagctat catgtataat tatatctcca
    13681 attttttctc tttttatacc tagacccagt attgacccta agtaatcttt atgagatata
    13741 tttttaaatt tgaaattccc ttctatttgt aaaaatctaa gagtatcttc tatatcttca
    13801 tattccatgt aaaatggata aataaataca gtgcttcttt cagcttgttc ataacctcca
    13861 tccacactgt attttatttc atttgtagaa tttaatattg ctacagcatt tttcacttca
    13921 tatggattaa gaaactccgt agacttaaca tcatagtttt ttatacaact attagccttg
    13981 tctatcactt taaacatttt atttttcaag tctatatcct ttatgtgatt tgttagtttt
    14041 aatttatcca tcctatcacc ttagaatata accaatacta attgtcttag gaacatcaat
    14101 aatactattg caattaaagg agaaaaatct ataggtccat ttgtatattt atacattata
    14161 gaccttattg gcccttctat tggctctgta agattttcca aaatatcaaa aattttactt
    14221 tgtcttccat ttggaaacca agtcatcaag ctttttataa ctatcatcca tgctattata
    14281 tctaaaagat aataaagtgc aattcttatt gttcccatat attaaccgcc tttttactat
    14341 ttattttgcc aagggaagaa tgctttactt tctaattctt ttttcatatt tgcatctata
    14401 tccacattat taggagctag tataaatatt gatttagaaa ccttttgtat acttccatct
    14461 aagacataaa ctgccccatt cataaagtta aatatagatt tacgaacttc agaattagtc
    14521 aatccttcca aattcactat aactgccctt ctttgtttta gatggtctgc tatagttgca
    14581 gcttcatcat aaaccttagg ttcaactatt acaactttca tttggctagt tgtatgtaaa
    14641 tttacaattt tatttgcttt tgcagtacta aagccactgt tcatttcttc ttcttgaact
    14701 atatcgtcca tgccactttc atattcatct tcaatgtaat cttcttcttc atcaactatc
    14761 caattcttaa atttagatat tattccattt gacattatta acttcctcct taaaatttat
    14821 tcaaatttga gtaattattt tttattgtag tttctttctc caaagattga tgttccaact
    14881 ctaacaatgg tagccccttc ctctatagca atttcataat cgtgactcat tcccattgat
    14941 aaaacatcca tccctacatt agggatgttt agttctttta tttgttttga taaatcttta
    15001 agacctttaa atacacctct tatctcatct tcatcttcta taaaaggtgc cattgtcatt
    15061 agacccttta tttttatatt tttatatttt tctgacactt ctttgacaaa gtctacaact
    15121 tcttctctat caagtccatg cttagactct tctttagata tattcacttg tactaagcaa
    15181 tctatttctc tgtttatttg ttctgcacgt ttttgaattt cttcgcataa tgcaattcta
    15241 tctaaagagt gtatcataca aactttatcg attatgtact taactttatt ggtttgcaaa
    15301 gttcctatca ggtgccagtt taatttatca cctataatat catactttct agctaattct
    15361 tggggtttat tttcaccaac agaagtgatt ccttccctca ttgcctcatt aactaagtct
    15421 acatcaacag tctttgtgac tgccaaaagg gtaatctctt tgctgtctct atctactttt
    15481 tttgcagctt tatcaatttt gtttcttatc tcagacaaat tatctttaat agaacccatt
    15541 attatcacct actttatcct tatatttgtg tttatactgt taggtcttaa aattattttg
    15601 tcatatagat taacacttgt aactccattt atgtcatttt tataataatc cacaaataca
    15661 gaagattcat tctcataagt tattccttct aatatgacaa attttacact ttgtgattgt
    15721 tcatcaatta catatacacc acgtttattg tcttttgttt ttatagaagt tttaggtatt
    15781 tttaaacatt ccatctgatt atatattata tcaaattctt cgacacttgt atcctgattt
    15841 gcaacaattt ggttacttat ttttaatttt accattattt tattattatt ttcatatatt
    15901 ttgtaaactt ttgcatctaa tttttgctca tgatattcaa ttattacatt ttgatttttt
    15961 tcaaagttat tagaatcttc tttactgatg tatgtagata gataattact ctggtcattg
    16021 gcatcttttt ctatacttcc ttttacatga atctttgcat caactttttc attttttaac
    16081 accaacgtat ttacattttt gctaattatc attactaata tattaaatac taaatatata
    16141 agaataccaa gtatcactaa tctggtatat tttacctttt tcaaagactc tccccctata
    16201 aaattacttt cattataagt acttcttcta aaaaatgttt attccttttt tataagctaa
    16261 tattttacgt tctaataatt attttttcca tattaaattt attttttatc aatcttccta
    16321 tacaagactg cattatttat atataattgt attataaagt ataagtttaa tttacatttt
    16381 atacgttaag cttactttat atgtttttat agttattttt atttttagat acaatataaa
    16441 accaattaag atttaaaata acttttgtta tgttgacatt tatttaggta atcatttacg
    16501 aatcaggata acgttatcat aacaaaagtt tatgtcctaa aaaaaataca aggaggattt
    16561 tcttatgtca gaaattttat taggaacagg tttactttta ttggttctat gttgtttcac
    16621 aatttttagt tacaaggccc catatggtat gaaggctatg ggagctctag caaacgctgc
    16681 atgtgctact ttcttagtag aggcattcaa cctttcttta cttggtgatg ttttaggtat
    16741 ggaatttttt agaagtgttg gagctgctaa tggtagtctt ggtggagttg ctgctgctat
    16801 acttgtgcct ttagccttag gtgtatctcc agtttatgca gttttaatag gattatcttg
    16861 tttagatttt tctatattat ctggattcgt tgctggatac tttgtttctt tcttaattaa
    16921 gtttatagag aaaaaagtgc catctggagt ggatttagtg gttgtaattc ttctatgtgc
    16981 accactatct cgtcttatag catcaaatgt agacccatta gttaatagta cactactaca
    17041 aattggacaa gttttgatag aagctaccaa cattagccct ataataatgg gaattatact
    17101 aggaggtcta ataacagtag ttggaacgtc tccacttagt tcaatggctc ttacatctat
    17161 aattggtctt actggtcttc ctatggcaat aggagcatta ggtgcttttg gttcagcttt
    17221 tatgaactca acactatttg caaaattgaa attaggttct aaaaaagata ttatatctgt
    17281 tgctattgaa ccattaaccc aatcagacat aatttcagct aatccaatac ctatatattc
    17341 tactaatttc tttggaggag ccttttctgg tatcattgta gccttgatgg gtcttgtaaa
    17401 catgacacca ggtacagcta cacctgtggc tggttttgct ataatgtttg cttataatcc
    17461 agttcaacaa gttcttataa cagctggttt atgtgcttta ttcaatgtaa ttgcaggatt
    17521 tttaggttca aaaatattca gtacctttaa agttcttact gctggtgaaa tacgtggtac
    17581 tgatgtagaa gaagaaattg cttaaaaaac aataagattt ataaatttat atctcaagta
    17641 gtagagtttt atattgtagg caattttatt aattgcaaac gttttatttt ttatttatgt
    17701 ttatgattat tatgtgtgtt gaatatttag ataaattttt atttattatt tttatttaga
    17761 tttttgttta gttttttatt tggttgatat attaggaata aaaattagta tagtttatta
    17821 ctagttagat agcataactt tttagataca taaaaaattt tatattaatt agatattatt
    17881 aaaataaaag cgatacatga atttttaaat gtccatgtat cgctttattt ttgccataat
    17941 cttaacaact gatttttaaa gtttattatc ttaacaaagt tcattatctt atataatatt
    18001 taaatgcata gacttcttta atttaaccat ctaaatattt aattaaatat tattatatgt
    18061 aatataacta atttttaaat gaatgtatag gagctggaat ccttcctcct ctttgagtaa
    18121 ataattcaga tgaatttgta tttataggca ttactggtgc tcgtcctaaa agtcctccaa
    18181 attctaccat atcacctaca tcacatcctg gggctggtat aattctaacg gctgtagtct
    18241 tattattaat catacctatt gctgcctcat cagctatcat ggctccaagc gtacctgctg
    18301 tagtatttcc tggcacagct atcatatcaa gacctacaga acaaatagca gtcattgctt
    18361 caagcttatc tatacttaaa gcacctttaa taacagcatc aatcatacct gcatcttcag
    18421 atactggtat aaacgctcca ctaagtcctc caacatgtga acatgccatt actccacctt
    18481 ttttaacagc atcatttaaa agtgctaatg ctgctgtagt cccatgagta ccaacaactt
    18541 caagacccat ttcctctaat atatttgcta ctgaatcacc aatagcaggt gtaggagcta
    18601 gtgataagtc tattatccca aatggaacat ccaatctact agaagcttcc tttgccacaa
    18661 gttgtccaac tcttgtaatt ttaaatgcag tctttttaac tgtttctgaa acaacatcaa
    18721 aaggctctcc ctttactttc tctaaagctc tcttaacaac accagggcca cttacaccaa
    18781 catttataat tctatcagcc tcaccaacac catggaaagc tcctgccata aatggattat
    18841 cttctactgc attacaaaat actactaatt ttgcacaacc aaaacctttt gcatcttttg
    18901 ttaattctgc agtttctttt atgatttctc ccatttcttt cactgcatcc atatttatac
    18961 cacatcttgt agaacctaca tttactgaag cacatacttt attagtactt gctagagctt
    19021 ttggaattga cttaattagt atcttatcac cttttgtata tcccttttga actaatgctg
    19081 agaatccacc aataaaatct atacctaaat cctctgcagc cttatccaat gtctgtgcaa
    19141 actttacata gtcattttca tctgttgcac ctgcaataat ggaaattgga gttactgata
    19201 cccttttatt tacaattggg ataccaaatt cacgctctat atcttttcca accttcacca
    19261 aatctttagc agaactcata atcttattgt atattttttc tctagcttta tctccatcag
    19321 ggtctataca atctaataaa gaaatcccca tagttatagt tcttatatcc aactgttctt
    19381 catctatcat ctttattgtt tcaagtatat ttcctacatt cactattatc acctcccctt
    19441 actgtctact acagattatg cattgaatta aatatttctt ctcgctgaat ttttacatcc
    19501 actgataatt tcttacctct ttcaaccaag tcctcaaccg ttttctcaaa agaatttaat
    19561 gatttctcta aatcaactat cataatcata gtaaaataat cctgtaatat tttttgtgat
    19621 atatctacta tgttgatatt ttgcttgtat aattcatcac taattcctgc cactatacct
    19681 ggtttatcct ttccaattac tgttaaaatt gctttcataa taatatcctc ccttattttt
    19741 ttataaatct tatattttta caaattgaac tatgtgttaa catatatttc atttatttgt
    19801 tagtaaacca ctttttatgt atatatttaa tatattttaa tacctataca ttcatttgtc
    19861 aatttttttt caatattcta ttatttttat actatttaga aaattcatat attatatatg
    19921 ttttctcaac aacaaataca gttttcataa taatgaaagg tattacttag caatctcatg
    19981 attttctaaa taataccttt ctaaaaatta tttttttaat tttatgaatt taattatact
    20041 ttt
//
LOCUS       DAEMHZ010000063        19777 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000063 DAEMHZ010000000
VERSION     DAEMHZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.063"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            112..669
                     /locus_tag="KPK72_002170"
     CDS             112..669
                     /locus_tag="KPK72_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489383.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            complement(1019..1381)
                     /locus_tag="KPK72_002171"
     CDS             complement(1019..1381)
                     /locus_tag="KPK72_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489384.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(1815..2624)
                     /locus_tag="KPK72_002172"
     CDS             complement(1815..2624)
                     /locus_tag="KPK72_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2489385.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            2716..3339
                     /locus_tag="KPK72_002173"
     CDS             2716..3339
                     /locus_tag="KPK72_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBG2489386.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            3336..4661
                     /locus_tag="KPK72_002174"
     CDS             3336..4661
                     /locus_tag="KPK72_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2489387.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            4682..5221
                     /locus_tag="KPK72_002175"
     CDS             4682..5221
                     /locus_tag="KPK72_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2489388.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLVIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            5240..6631
                     /locus_tag="KPK72_002176"
     CDS             5240..6631
                     /locus_tag="KPK72_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBG2489389.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFADYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVETRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            6646..7737
                     /gene="ytvI"
                     /locus_tag="KPK72_002177"
     CDS             6646..7737
                     /gene="ytvI"
                     /locus_tag="KPK72_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2489390.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIISLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFAIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     misc_binding    8040..8253
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8507..9541
                     /locus_tag="KPK72_002178"
     CDS             8507..9541
                     /locus_tag="KPK72_002178"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG2489391.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            9582..10799
                     /gene="argJ"
                     /locus_tag="KPK72_002179"
     CDS             9582..10799
                     /gene="argJ"
                     /locus_tag="KPK72_002179"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG2489392.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            10814..11674
                     /gene="argB"
                     /locus_tag="KPK72_002180"
     CDS             10814..11674
                     /gene="argB"
                     /locus_tag="KPK72_002180"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG2489393.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            11704..12891
                     /locus_tag="KPK72_002181"
     CDS             11704..12891
                     /locus_tag="KPK72_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG2489394.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            13000..14022
                     /gene="argF"
                     /locus_tag="KPK72_002182"
     CDS             13000..14022
                     /gene="argF"
                     /locus_tag="KPK72_002182"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG2489395.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            14149..14766
                     /locus_tag="KPK72_002183"
     CDS             14149..14766
                     /locus_tag="KPK72_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG2489396.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSIKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(14986..15684)
                     /locus_tag="KPK72_002184"
     CDS             complement(14986..15684)
                     /locus_tag="KPK72_002184"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG2489397.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNDIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(15846..16235)
                     /locus_tag="KPK72_002185"
                     /pseudo
     CDS             complement(15846..16235)
                     /locus_tag="KPK72_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(16341..17507)
                     /locus_tag="KPK72_002186"
     CDS             complement(16341..17507)
                     /locus_tag="KPK72_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2489398.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVNGKRKKVEKVGGETKKEAEKALR
                     DAINEYENAGIVFDETNMSLSNYLDFWYKEYVLLNCKYNTQVNYRNLIKNHIEPELGK
                     YKLKSINPAIIQEFLNNKTKETYTQNGEERRYTRGNLKAIYGVLNSALKSAVYPYKLI
                     KENPAQYTNIPKNIIDTKKDSENKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNIDLDNNIIKVRKNLIKRKVSEFELTSPKTKTSIRDIRIGDTLSKVLKE
                     EKLNQKKQKIKLGKWYTETEYDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCL
                     RHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILENLVR"
     gene            17731..19290
                     /locus_tag="KPK72_002187"
     CDS             17731..19290
                     /locus_tag="KPK72_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2489399.1"
                     /translation="MKLKKLLSLGLAMSMFIVACPVSANALDKIDKIQGADKYETAGI
                     IADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRIEKAKKV
                     YIIGGESSIDKATETFLKDKGIETRRLQGSDRIKTSYIVAKEINSINKVNKVILTNAF
                     KGEPDAMSIASVAVRDKVAIVLTDGKSVPFNTTGIESYAIGGTSSMSDNLVNDTNSTR
                     LGGVDRYDTNKKIINKFYNGAKEFYIASGTDLVYALVGSTIAKNTPIVLVDDNSNKTA
                     LKGATKITAIGNLNNNAIEQSLNITKNIASPTTGGKYTVKEATERIAYILDEKVFVDY
                     ESAIKAKTDPDECFRYYIKNFTYQGKDYYAIGADDDDFRFLVNMNNLNDILQWYSNDT
                     IKRFDAEGYLKERKAIDMAFEACSSKYGISEDDLILDREEHNISTDFYVVVLYESSGI
                     DSYWVFIDTDTWTVHDIQRLNQNEELVSYHDVNPNTEADKHYNRDVDDVNIIFVPSEN
                     NPFDNDVDGIN"
BASE COUNT         7312 a         2235 c         3386 g         6844 t
ORIGIN      
        1 gtcaagattt tataatagta taaagaattt tataaattac aataattgac gaaactaatt
       61 agattagcta aactaataaa gtaattaatt ttaaatgaaa gagggaaaat catgcgtgtt
      121 agtagagaaa tagttataca agctacatca gatatagtta ataaagatgg gcttaacaaa
      181 gtttctttaa aagttattgc agaaaagcta ggaatacgta caccatcttt gtacaatcat
      241 atagaaagtc tagatgattt attacttgaa gtagctcata aaggaatgag agagatgaat
      301 agtcaaatga taaaggcgac tattggaaat tttggagata cagctataaa gtgtataagt
      361 atagcatact ttaattttat aatttctaat cctggtgttt atgaaacaat tcagtgggct
      421 acttggcatg gaaataatga aacattagaa atctttgatg aatataaatc cttattagaa
      481 aaacttattc tttcttgtaa tctaaataca aagaatacag gtgaagtatt aaacttaata
      541 atgagtgtac ttcatggtta ttcgactttg caacttggga aatcactttt gagtaaagaa
      601 gaagctataa aaggtctagt cgattctatt gatactgtgt tattaggtat tcataaaaaa
      661 tatgattaag gaggtaaata aattatttta acttatttat tgaacacttc attacgtggg
      721 atgtaaaata aaattttatc atttttatga aaggaaaata gtatatttca agaatattta
      781 ctttctgaat tggtaaaccc tatttttact gttgttgttg ataatgaaaa ggtagctgta
      841 aaattccttg ataatcagac aaaagcgaca caggtatcat agtataaatt gatatttcat
      901 aaggacagta attggaaaat tattggatag gttatttgat tatataaaaa tgattatata
      961 aaaaaagaaa agaccttagc taacaacaca tatcaattta taccttgaag ctgtggtttt
     1021 atttttcaaa ttgtttgcgg atttctctgt ctttacggat tacagttttt gctgatttta
     1081 gcagttcaac taaagaacgg atttcatatt catcacaatc tgagaaaata tctttggctt
     1141 ccttttcaaa aattactttg gaagaaagta cattatcaca taagagagta tcgactgata
     1201 cagaaagtgc attggcaatc gcaatcaagg ttggcaggct gactttcgtt tttcctgttt
     1261 ccacgttgct catatgggat ggtgtcatgt tgataatgtt tgcaacggat tcttgtgtta
     1321 tacctttttt aattcttgca attttaattc gctgtccgat tgctttataa tcaatttcca
     1381 tagtttttgt tctcctttca ttcttttaaa tattgtaact atttacagag cataatataa
     1441 tactctatat aaatgaataa tttatataat atatatttaa gaaaaaaatt tgcactactt
     1501 ttaagaaaac tacaaaagaa atggaggcat aaagcctaca tgttaagatt ttataaaaag
     1561 atagtaaaaa actgattaaa tggttaacga aaataaaaag ctatttctgt atctgaaatt
     1621 catgaggaaa tgcagtttgc attgaggaaa caaataatag caatgaacct gaatttctac
     1681 aaaaatttgg aaataagata ccaactttag aagataaaaa aggacatttt aagcaaatta
     1741 ttatagacaa tacgagatat taaaaaaggc gtgtatttct catgtgattt gagatataca
     1801 cgctttttat attttcaaat aacctgcata gaaatttttt gaatgtagtt ttcggcgtta
     1861 tgttctgata aataatcaat taaatcttct tcataaatat ttccaacaat atgataccct
     1921 tgttcttcta actgattttt catattttga taagttttat ataaactgtc ataactcccc
     1981 tggtgataca taactgcata atttcccttt ggtttaataa aaaggcgctc atcatctact
     2041 ttatttttaa taaggctata ataatagctc tctctaaaaa ttccctgttt tatatttttt
     2101 tcaagaacaa tttctcctac atgaaaatta taatcatgta ataaacaata ttgtaagtga
     2161 tttccaatac cttgtaacat tgttttttca tctgttagta cagcttcagg aattttagtt
     2221 gcaatcagat attcttcctc acaatttaca atttcgattt tttctgtgtt tacattgaga
     2281 tgtatttcca ttgttgaaat gacatttctc aacatagaac acattttttc aatcttcttc
     2341 acttcttctt gaagatccat tagctttctg tgaagtacat taagaaaggt attcgtatct
     2401 aaatcattga gatattgttt tatttcttct aaaggtgtac ctgctttttt tagcacagaa
     2461 ataatattaa atttttcaaa ttggtcaatt gtataatacc gatagccatt ttcatgggta
     2521 atactcggtt ttaataattc aatttcatca taatggtaaa gtgtatgttt ttttacccca
     2581 cacaattctg caaattcact tgtcttatat aattttttaa tcatttttaa atctcctatt
     2641 gactttaggg ttaccagata ctttaggata acatagaaag cttttatatt caatgaaatg
     2701 tgaggaaaag atattatgaa aattattaca attagccgag agtttggaag tggtggacga
     2761 gaacttggaa aacaacttgc tgatatttta aattttgact actacgataa agaaattatc
     2821 actgctatag ctagaaagaa aaaaattgac gaaaactatg tttcctacat attagaaaat
     2881 catggttgga gaaacgttcc tttaacaatg tgtacttctt ttactaatat tttgtctatg
     2941 caatccatga agacagattt gttaatagaa caatcaaatg ttatcaatca gattgccagt
     3001 acaggtaagg attgtgttat tgttggtaga aatgcagata tattattaaa gaagtaccag
     3061 ccatttaacg tttttgtttg tgcaaatatg aactttaaaa tacaacgctg tattgaaaaa
     3121 aagttaaaca atgaacatat ttcaaaaaaa gaattagaaa gaaagataag agagattgat
     3181 aaaaatcgag caagatcaag agaatttttt acgagctcct tatggggaaa tcaaacttcc
     3241 tatcatatta tagttaatac atctgaatgg aatataaaac atctttctta tgcaattgct
     3301 aacttttcag agtattggtt taggaggtat gaacaatgat acagttgtca gataatttta
     3361 attataaacg cttattaaaa tttacatttc ctacaattat tatgctggta ataagttcta
     3421 tttatggggt tgttgatgga tattttgttt caaattttgt agggaaaact gcttttactg
     3481 ctgtcaattt tattatgcca tttttgctaa tcttagggtg tgttggcttt ctttttggaa
     3541 caggtggtgg agctttaatt gctaaaacaa tgggtgaagg aaaaaaagaa gaagcaaacg
     3601 aacagttttc gttactcatt gttacttctg ctggttgtgg tattattcta gctgttttag
     3661 gtatagtaac tatgccatgg attgcttctg ctatgggagc agatggtcaa ttattgacag
     3721 atagtattct ttatggatgt gtggtaatta tcgctattcc tgcctatatt ttgcagtgcg
     3781 aatttcagtg tttatttgca acagcagaaa agccaaaact tggattatat gttgcaatta
     3841 tggctggaat tacaaatatt gttttagacg cattatttat cactgttttt aaatggggat
     3901 tggtaggtgc agcactggca actgcaatcg gtcaatatgt tggtggtatc attccattac
     3961 tttattttag ccgacaaaac aacagtttgc ttaaatttaa gaaacctaaa tttgattgtg
     4021 atgttttgaa aaagactgtt gcaaatggtt cttcagaact aatgactaat gtttccacat
     4081 ctgttgtgag tatgctgtat aatgctcaat tactaaaata tgctggcgaa gatggcgttg
     4141 catcctatgg tgttcttctt tatgtagcgt tactatttca agctgttttt attgggtatt
     4201 ctgttggtac tgcccctata atcagttatc attatggagc aaaaaattat gacgaattaa
     4261 aaaatttaag aaaaagaagt ttgattgtaa ttatttgttt tgctgtattt atgtttataa
     4321 taggcgaatt tttatcaaaa ccgattgctt ttatttttgt gggttacgat aataatttaa
     4381 tggaaatgac attaagagca tttatgattt actctttttc ctttttattt tctggttttg
     4441 gtgtttttgg ttctagtttc tttactgcat taaatgacgg tttaacttct gcgcttattg
     4501 cttttatgcg tattttagta tttcaagttg tatcagtact tatattccct atattctggg
     4561 gagtaaatgg gatatggttt tctattgttg gtgctgaaat attggcaact attatgacgg
     4621 ctttctttat aataagaaaa cagaaaaaat atcattatta aatcaatttg gaggtatcta
     4681 tatgagctta acaatacaaa aagtaacttc atattttaaa gatttagaaa aatttgaaga
     4741 acttaataac gaggcatttc cagatgaaga acgtatgtct atacaagaaa tgcttaattt
     4801 agttcaaagt aaaaaaatag aagtatctgc tttatatgat aagcaacaat ttattggttt
     4861 ttatgttttg gtgattgaaa atcatatagg atatatatta tttcttgcaa tttctccaga
     4921 acaacgctct aaaggatatg gtggaaaagc actgtcactt atgaaaaaac aatatcccaa
     4981 ttgtcaaatt gttcttgata tggaaactat agaggacagt gctcctaatt tacagcaaag
     5041 aattttaagg aagcaatttt atcttcagaa tgggttttat gaaacagaat atctcatgga
     5101 gtataaggga ctagaaatgg aagttttatg taatcagaaa acctttcaaa aaaatgaatt
     5161 tgaaacattg ttgaaaaatc ttaaaattag agaaattcca tttcatttgt ggaaaaagta
     5221 agaaagtgag gtattagaaa tgaaaagacg tttagctggg gaatgggaac cgattaaagg
     5281 tgtcatgctt gcatggccac cttttcttcc ccataagtat attgtagatt taacacaaaa
     5341 ttattctgta tacatgtgtg taccaaataa agaggaaatt gaaattgcaa aatattattt
     5401 aagtaagtgg ggtggaaatt taaaaaattt gattttctgc attgctccac agggatttga
     5461 tacaccatgg gttcgtgatt ggggtatgca ccctattttt gatgaaaacg gaaaactctt
     5521 tttggctggt gcagaatata agatgtcaac accatttgta tccataaata aaccagaaat
     5581 catgtttgat tgcgaacata acctattaac tactaactct tatgaacaaa aagaagatca
     5641 atcacaagag ttgattgcga aacagattgg tttgccattt atgaaattac cttatgcact
     5701 aacaggcgga aatcttatgt cagatggttt caataaattg atgtctatgc acattttaca
     5761 gacagaaaat cgtgcgaaag gaatttctga cgaagaattt ttttctatgg tggcaagaga
     5821 tactggaatg aatgagtatt atatattttc taattttgct gattatggtt tacaacatat
     5881 tgactgttat ttaaaaataa ttgatgaaga aacattactt gtgagcagaa cgccaaaaga
     5941 acactatttg tatgaaagat atgaaaaaat tttagaagaa gtaaaaaagg tagaaaccag
     6001 atatggacgt ccattcaaaa tctttcgtat agatattgtt ccgtttcaaa aggacagtga
     6061 tgaacttact gcttatgtta attctgtgat tttaaatagt cgtgtctatg ttccaatgta
     6121 tggaattgaa caggataaaa ttgcaataaa acaatggcag aacgctttgc ctggatatga
     6181 aattaaagga tatgagtttg attttgaaaa agagccagat attataaaaa atcgtacagg
     6241 atatgtgaga actggctggg gaaatgaaga tgttattcat tgcaggacaa gagcaatttg
     6301 ggatgagaat atgctctata tttccgtgaa aagattagaa gaaatcgttt cagagaatga
     6361 tgctttagaa gttactattc aaatagttga ttatagtaaa gctggcttag attatgaaaa
     6421 ttgtagactg ttttatcgat actatggttt tcaaacatgg gaaagtatta ggttagaaga
     6481 aactacagaa gcagaaatat tttttgccaa tatgatagga aagtcaggtg atatgattga
     6541 atattttgtg caagcaaaaa gttgttctgg aatgtgtgaa acaatgcccc ctgttgctcc
     6601 aaaaggcaca tataaagtta ctataaagta ggaggtgcat tttatttgaa tgaacaagat
     6661 catacatcat accaaaataa gcagagattt ttaataaatt tctgttattt ggtagtgata
     6721 ttaggcttac taatgttatt agggaaatat gcgttttctg tattacttcc ttttgttttt
     6781 gcatttttaa tagcaagttt attgaataaa ccagtcttat acataagtga aaaattacta
     6841 ataaagcgag gtattgtagc aaccatatct gtttttttat tctttttaat tgcaggaata
     6901 atagtaagtg taataggtac atatctaata tatggaataa aggaaatttt tcatttccta
     6961 ccaaccatgt ttgaggaatt ggtaatacct ttgactgaaa cagttataaa agaagtcgaa
     7021 ctattttctg gttcattcaa tttttcgttt attgaattaa tagaagcaaa tatgcctgta
     7081 ttgatgaatg aaatgagtgt tttcataatt tctctttcaa ataatatcgt atctggtatt
     7141 actgatattg tttcgtctgt tccattacta tttatgaaaa caattattac aatcgttgct
     7201 tctattttta tagcaattga ttatccaacg ataaagagat ttatagtgct acagatacct
     7261 aaaaataaaa gttatctttt agcagaagca aaagcgttta ctattgatac cattattaaa
     7321 tgtggttttt cttatctttt aatttttgct atcacttttt ctgaattata tgtcggtttt
     7381 gctattatta atatttctta tgctggaata attgctttat tcattgctct tttagatata
     7441 ctacctgttt taggaactgg aagtattcta ataccttggt gtattatcag tttattttta
     7501 ggggattatt caatggcatt aggaattgca ctactatata ttgtgattac tattatcagg
     7561 aatattattg aaccaaaatt agttggtaaa catatggagt tacatcctgt attggcctta
     7621 gccagtatgc taacaggatt acatttcttt ggatttattg ggttgttcgg tattccattg
     7681 ttaattgcgt ttttgaaaaa gttaaatgac aaagaaatca tacatatatt acattaggaa
     7741 agaatagttg tatgtatgtc tcttttatgg agaaaattgt gttttttcta aaaaacgctg
     7801 gatgaaaaat aattttgaca aaggtatagc ttttgaatga cttgattcaa gagggagaac
     7861 ttttttaaaa tatattacag caggaaattc atggtgtcct attactgtag atatttataa
     7921 ctcaatttag atacaaaatg ctggaaatta attatataga aaatatttcc aaaaatttaa
     7981 aaattatatt gacaaagtta aaaaaataaa ctatcatata aaacaataag aaaataaatt
     8041 gaaatcctgt gaaaaagaga gtagcataaa gctagttgat agagagctgg gaatggtgga
     8101 agcctagtaa cgaattttat tgtgaataga gctttggagg agattaccag aagtaatagt
     8161 atggtaattg gtgaatctcg ccttaagaga ttagagtggg taagattatc ttatcaatta
     8221 gagtggtaac gcgggtgtat attcgtctct tggtctttga ccaagaggct tttttaattt
     8281 taaatgtaca ttttgtagat actttttaat ttgagtttta atgattattg taattttacc
     8341 aataagagtg gtaccgcgga aaatcgcctc ttagtattta taatgctaag gggttttttg
     8401 ttttaattag agtattttaa tctacgtatt atcaaatgat tatcatatag taaagtggaa
     8461 agataggatt ttaagactat ttgaatataa aaatggaggg aacaaaatga ataaaataaa
     8521 agttggtatt attggtgcaa ctggatatgt cggggcagag ttgattagat tattaatgaa
     8581 tcatgataaa gtcgaagtga cagcgatagg ttcaaattcg tatgttggaa aagatatagt
     8641 tgatatatat ccaagtatag gatataaaaa taacatgata tgtatagaaa atgaaaaagt
     8701 tatagatatg tgtgatgttg tatttacagc ccttccacat ggagttagtg aaaagtttgt
     8761 aataaaagca attaaatcga aaaagaaagt tatagattta ggtgctgact ttagaataaa
     8821 agatgaagaa gtatatagta aatggtatgg agtatctttt atagataaaa tcttacataa
     8881 aaaagctgta tatggattaa gtgaaatata caaagaagat ataaaagatg cagatataat
     8941 tgcaaatcct ggatgttatc caactagtat ttctttacca ttgatgccat tactaagttc
     9001 taaattgata aaaaataata atataataat tgattctaaa tcaggtctta caggtgctgg
     9061 tagagaatta tcgattagta gtcactttac ggaggttaat gaaaatatca ctgcatataa
     9121 aatagggaaa cacagacata ctccagaaat agaacaaaat ttaagtgaat cctgcaaaga
     9181 aaaagtaagt gtagttttta caccaaactt aatcccagta aataggggaa tcttatcaac
     9241 tatatattgt acaaaagaag ataatatttc tataaatgat atacatagga aattaactga
     9301 ttattatgaa tttaaagaat ttatagaaat tcttccatta gataaggtag cttctttaaa
     9361 gaatgttaga ttctcaaata aatgtgtaat atcattacat gaaaatggag atacattgat
     9421 aatctgttca gctatagata atatgattaa gggagcagca ggtcaagcta tacaaaacat
     9481 gaatataata tttggtattg aagaaaatac aggtttaaaa aatattgcac catcatttta
     9541 gaaataaaaa atactaaact catataatta ggaggataaa tatggagatt ataaagggtg
     9601 gagttacagt ttcagaagga ttttttgctt cagggattca ctgtggcttg agaaaaaata
     9661 aagaaaaaag agatttagct ttggtttatt cagatgtttt atgtgatgca gcagcagtag
     9721 taactcaaaa taaagtaaag ggcaatcctg tttatgttac acaggaacac attaaaaatg
     9781 gaaaagcaca agctatcata gtaaatagtg ctaatgctaa tacctttaat ggaaaagaag
     9841 gacttataaa tgcttataaa atggctaaat tcaccagtga taaattaaat attaaagaaa
     9901 gtgatgtttt ggttgcatct acaggtgtta taggaaagcc attaaatata gatttaatag
     9961 aagaaaatat ggatgaatta gtgaataatc taagtaaaca aggtcatatt ggagcaagag
    10021 aagctattat gacaactgat attataaaaa aagaaatagc agtagcaata atgtatggag
    10081 ataaaaaaat aactattgga ggtatggcaa aaggttctgg catgatacat cctaatatgg
    10141 caacaacgtt aggatttata actacagatg caaatattga tggagttttg ctaaaagaag
    10201 ctttaaaaat tgctgttgac aaaagtttta atagagttag tgtagatgga gatacaagta
    10261 ctaatgatat ggtaatgatt ctagcaaatg gaaaagcaaa aaatgataga ataaacaaaa
    10321 aagatgaaca ttatcaagta tttttgagtg cacttacata tgtctgtata gaacttgcta
    10381 aacttgttgc aaaagatgga gaaggatgta caaaactaat tgaatgctat ataaatggag
    10441 cattaagtga agaacatgca gtaaaattag caaaaacagt tatatcttct tcattagtaa
    10501 agacagctgt atttggagct gacgcaaatt ggggtagaat actgtgtgct ttaggttatg
    10561 caggtgaaga aatagatatg gaaaaagttg atattatttt tgagagtatg aaaggatata
    10621 ttgaagtatg taaaaatgga aatggattag attttaatga ggaaaaagca aaaaaaatcc
    10681 ttgaagatga cgaaataagt atattagtag atttaaatat gggcaatgcc agaggaaatg
    10741 catggggatg tgatttaagc tatgattatg taagaataaa tggaagttat agaaattagg
    10801 aggatatact ttaatgatta atatagaaaa agcaaataca cttatagaag ctttaccata
    10861 tatagaaaaa catcagggaa agactattgt agttaagtat ggtggaagtg caatgaaaaa
    10921 agatggtcta aaagaatctg ttatggaaga ccttgtgtta atgagttatg ttggaatcaa
    10981 tatagtttta gtacatggtg gaggggcaga aatcaataag atgctagcaa aagtggatat
    11041 agaaagtaaa tttgtaaatg gtcttagata tacagatgaa gagaccatgg aaattgtaaa
    11101 aatggtactt gctggaaagg ttaataaaga cttagtaaat aaaatacata caaaaggtgg
    11161 aaaagcagta gggctttgtg gaatagataa taatatgatt ttatgtgacc catataaaaa
    11221 ttatgaactt ggatttgtag gagaaatcaa aaaagtaaat gttgagctta tagaatcttg
    11281 tctaaagtca ggttatattt cagtaattgc aactataggt gttggagatg atggagagac
    11341 ttataatata aatggtgaca cagcagcttc tgctatagct aaagagttaa atgcagataa
    11401 attgatactt cttacagatg tccctggttt gttaagagag ccagatgaag aaaaatcatt
    11461 aataacagaa gttatattag aggatgttga taaattattt gaagaaggta taattactgg
    11521 aggtatgata cctaaaatag agggatgcgt agatgcctta aataacggag taaatagggt
    11581 acatattttg gatggaagag ttccacattc tataataaca gagctattta ctgatagtgg
    11641 aataggtaca ttaataagaa aagaaaatga ataaaattat aagtttaaga ggggagaata
    11701 agaatgaata acgacaatac aatttcaaaa tggaatgaat attttataga tacatataac
    11761 caacccaatt ttgtcataga ttatggtgaa ggaagttgtt tttttgatac taatggcaat
    11821 aaatatatag attttacaag tggatatgga gtatcttctc ttgggtatag taatagtaat
    11881 ttaaagaatg ctctaaaaga gcaagtagat aaattattac atacttcaaa tttatatttt
    11941 aatgagccag tgttgtattc aggagagaaa ataattaatt caagtggaat ggctaaagta
    12001 tatttttgta attctggaac agaagcaaat gaaacagcat tcaaaatagc aagaaagtat
    12061 agtagtgaca aatatggtaa tggtagagga actatcataa gtttaaaaga ctcttttcat
    12121 ggtagaacaa tgatgtcttt aatggcaact ggtatggaca aataccataa gtatttttat
    12181 cctcttcccg aaggttttaa atatatagaa agaaataata ttgaagattt aaaaaataat
    12241 cttgattcca cagtttgtgc aataattctt gaagcgattc aaggcgaagg tggagttaat
    12301 gttcttgaaa aagattatgt tttggaaata gtaaaaatat gtcaagaaaa ggatattgta
    12361 gtgatttttg atgaagtcca atgcggtata ggaagaactg gtaaactttt tggctatgaa
    12421 tattttgatg taaaaccaga tatagttacc gtagcaaaag ggttgggagc aggaatacca
    12481 gtaggaggag tattggtaaa taaaaaactt tcaaaagtac taggaaaagg agaccaaggt
    12541 acaacttttg gaggtaattt attagctatg gtggcagctt cagttgtatt agatgagata
    12601 tctaaagatg gtttttataa tgaagtatta gaaaaaggaa attatataag aaaatctatt
    12661 gaaagtttta ataacaaagt tgtattaaag acaaaaggta ttggtctaat gataggaatt
    12721 gaaactaata ttgaatctag tattatagaa gagaaagcta gaaaaaaagg attgcttatt
    12781 ttgactgctg gaaaaaatgt attgagattt ttaccaccat taacaatcag ttataaggaa
    12841 atagatgagg ctcttgaaat cctaaaagat atattactag aaattaacta aatattcaaa
    12901 aaatagttga ttaaagataa aaattagaag taaataaaaa agtaaagtgc taacaagttt
    12961 aattgaattt aaaaagttta taagaagggg cgaattaata tgaataatat aagtttaaaa
    13021 ggtaagagtt ttttgacatt aaaggatttt acaaaagaag agattagata tttattaact
    13081 ttatcaagtt atttaaaaac taagaaaaaa gttggtataa aaggcgattt gctacaagga
    13141 aaaaatatag ctttattatt tgagaaaaca tcaacaagaa ctagatgttc atttgaagta
    13201 gcagctcatg atgaaggagc acatgtaact tatctaggtc caaatgattg tcatatgggt
    13261 aaaaaagagt ctgtatcaga tacagcaaaa gttctaggaa gattctatga tggaatagaa
    13321 tttagaggct ttaaacaaga aacagttgaa agtctagcaa aatattctgg tgtacctgtt
    13381 tggaatggac ttacagatga atatcatcca acacaaatac tggcagattt tttaacagta
    13441 attgaaaatg tagataaaga tttgaataaa gttaaatttg tatatgttgg tgatgctaga
    13501 aataatatgg gaaactcact gatgatagga tgtgctaaaa tgggaataca tttcgtagca
    13561 ctagcaccta aggaactttg gccaaatgaa gaattggtta aggaaatgaa ggaaatagcc
    13621 aagaaatcca aaggagatat aatgttgact gaaaatattg atgatgttaa aggtgcagat
    13681 gttatatata cagatgtatg ggtttctatg ggagaagagg agcaatatga agcaagaata
    13741 aatcaactta aagattatca agtaaatatg gatatgataa agaaaactga aaatgaaaat
    13801 gttatatttt tacactgtct tccagcattt catgatttag aaactaaggt tgctcaggag
    13861 atttatgaaa agttcggatt atcagaatta gaggttacaa acgaggtatt taattcaaaa
    13921 tattcaaagg tatttgaaga agctgaaaat agaatgcata caataaaagc tgttatggtt
    13981 tctactatga ctgatgtaaa tatgaacaat aatttagtat agatatgttt taatatagac
    14041 tttactttaa tgaaaattta taaaatattt taaatatatt attcaaaaac tctcattata
    14101 tgttaaaata acttatgtat caacaaatta agagggagga tttgagatat gaaaaaaatc
    14161 atattagctt taacaacttt tttattagca atatctttaa cagcatgttc aagtgcaaaa
    14221 ccagaagata caattgatag tttttttaac agtgcgaaaa agtttgactt tgaaggcatg
    14281 aataaggtaa tggaaaataa tgacgaaaaa tataaggata tattaaagga attagataca
    14341 aaagacccaa atgcccaata tgttttagat tatctaaaac agaatgcatc taaaatcaca
    14401 tatacaataa aagacagcga ggttaaaggt gataaggcta caatcaaagt agaatgtaaa
    14461 tttatagatt caacaccatt acttcaagaa atagtagctg aggcatttac aaagatgatt
    14521 ggtatgagtt tttcaggaca agacttaact gatgagaaaa caactgaaat gttagtatca
    14581 attatgaaag aaaaacaaaa aagcattaaa gagacttatg ttacaaaaaa tgtagagttt
    14641 gaatgttcta aaaaggataa taaatggata ataagttcag taaatgatgc agtagctgat
    14701 gtattgttat ctaatcttgt tacagcagga caagaatttt ctaactctat gaatacactt
    14761 gaataatatt gtttaggtgt agtaaagtaa taaatatatg attattttta taaataaaca
    14821 tataaatatt tatgaaggat accatttttg gtatcctttt tattaatata atgaaataca
    14881 tggttaattt ctatattgtc aagaaactat gatatatgtt ataagtctat actatttaag
    14941 ataaacttat aacatatatt ttttataata tagtgcatga aaaatttatt taactaattt
    15001 tagatattta ttgacaactg attcagccaa gcattttgat gaatcaacaa tatttattga
    15061 aggatatgat ttttctaaaa ctacatttaa atcagtacaa gcaattacta tattttctat
    15121 agattcattt ttaacatctt cgataagttt attccaaata tcaatattgt gtggattttc
    15181 tttatccatt tttatatttt gaataagatt gtttaatttt atttgccatt catctttaaa
    15241 tataaattca tgtccattat gtattatatc attctggtaa attgtagact caaaagttga
    15301 actagtcgaa aaaagagtta ctttttgtga gatagtaggt aattttttaa ctgtttcttc
    15361 tacaatattt aatattggta tattaataga ctcttttaat tctttaaagt atatatgtgc
    15421 tgaattgcat ggcattgcaa taaaatttac accagttgat tcaagctttt gtaatccatc
    15481 aattatagtt tctttcataa gttcatgatt tattggacgg tcaatataaa atggggtagg
    15541 taatgaatat atcatcatct ttggaaattc atcatcatat tttgctccat attgagattg
    15601 acactcatca ataattaaat caacaaaagg ggctgttgac cttggaccca ttccagccaa
    15661 aataccaatt aatttttcat tcataaaaat tatctccttt atatataaat atatttatgt
    15721 ataaaataaa aatctataaa agcataaaat tatattttat aatagattat attgtacact
    15781 tatataatat aaatataaag tgatttttta gattatatat tttaatatac tttcttatat
    15841 aagaattatt ttaaatcaag attatctagt atttttaaaa atatttttac agcagtatcc
    15901 aagtcatcta cagaaacact ttcattagga ttatgactta cgcccccagc gcatcttata
    15961 aataccatac caatatcagt tatattatcc atttcctgag catcatggcc agcaccacta
    16021 taaatataaa aaggattaag atttaaatct ataaaacttt tttctatgat ttttgttatt
    16081 ttattagaac atggtacagg aacattttca aaagcaagtt cacttgtata attaagtttt
    16141 ctattttcac atatatgtga tatttcatta aatatctttt ctacagaatt atcaagaatt
    16201 tcttgtgaac aagagcgtac atcaagagta aattcgatta ttctatataa aatggctaaa
    16261 tagctggatt taagtaaaaa aaatagactt tatcattgtt ttgccaccca tttgccaccg
    16321 taattttttt cggtggcaaa ttatctaaca agattttcta aaatatctat tgtttcactt
    16381 tccattttac ttgtaacatg tgaatatgtg tccatagtag ttgataattg actatgtcct
    16441 aatctttgtt gtatatattt tatattagct ccattttcta ataataatgt agcatgtgta
    16501 tgtcttaaac aatggaagtt gaagtcaagg tttaattttt tgttaattgt tctcacacaa
    16561 gcatcaatgt tgttatgatt tacaaatgag ccatcttttt ttctacaaac ccaatcatac
    16621 tcagtttctg tataccattt tccaagttta attttctgct ttttttggtt taatttttct
    16681 tcttttaata ctttagataa agtatcacct attctaatat ctcttatgga tgtttttgtt
    16741 ttaggtgaag ttaattcaaa ctctgaaacc tttctcttta ttaaattttt tctaactttg
    16801 attatgttat tatctaaatc gatattgtcc caacaaagac ctaatatctc tccttttctc
    16861 attcctgtat gaaaacctat aagtagagga atatagatat ttgtattttt aggatatatc
    16921 tctagaattt tattaaattc atctaatgtt atagttttgt tttctgaatc tttttttgta
    16981 tctataatat ttttgggtat gttggtgtat tgagcaggat tttctttaat aagcttgtaa
    17041 ggataaacag cagattttaa agcagagttt aatacaccat agattgcttt caaattacct
    17101 cttgtatagc gtctttcttc tccgttttga gtatatgttt cttttgtttt attgttcagg
    17161 aactcttgaa taatagctgg atttatagat tttaatttat atttgccaag ttcaggctct
    17221 atatggtttt taattaaatt tctataattt acttgagtat tatatttaca attaagaagt
    17281 acatattctt tataccaaaa gtctaaataa ttagataaac tcatatttgt ttcatcaaat
    17341 actataccag cattttcata ttcatttatt gcatctctta aagctttttc agcttccttt
    17401 ttagtttctc caccaacttt ttctactttt tttctcttac catttacaat acctgcatca
    17461 aaatagtaat accatttctt acctcttttt cttactccac ccttcataaa aatttctcct
    17521 ctcaaaatta aataatgtgg tcaaagtaaa atgtttcaat tatattataa caattatata
    17581 tattataaag aataaaatta taagaaaata atttattcta atgttatttg atgtcgttaa
    17641 ttgagtagaa atagtttgta ctttcgacaa catataatat atttttgtta tacaatttaa
    17701 ttaagtgata tgaaaaaagg gggagatttt gtgaaattaa agaaattatt atcgctagga
    17761 ttagctatgt caatgtttat tgttgcttgt cctgtatctg caaatgcact tgataaaatt
    17821 gataaaattc aaggagcaga taaatacgaa actgctggta tcatagctga caaacaaagt
    17881 tatactactg caatacttat aaatgcagat agtacaatgg ctgatgggct aagtgcgagt
    17941 ggacttgcag gagcaacaaa tgcaccaatt cttttaacta agaaaaataa tattcctaat
    18001 gcaactctta aaagaataga gaaagctaaa aaggtataca taataggtgg agaaagttct
    18061 attgataaag caacagaaac ttttctaaaa gataaaggaa tagagactag aaggcttcaa
    18121 ggtagtgata ggattaagac aagttatatt gttgcaaaag aaataaactc aattaacaaa
    18181 gtaaataaag ttatattgac taatgctttt aaaggagagc cagatgctat gagtattgct
    18241 tctgtagctg ttagagataa agtggctata gtattaactg atggaaaaag tgttccattt
    18301 aatactactg gaatagaaag ttatgcaata ggtggtactt catcaatgag tgataactta
    18361 gtaaatgata ctaactcaac tagattgggt ggagtagata gatatgatac taataagaag
    18421 ataataaata agttctataa tggagcaaaa gaattttata tagcaagtgg aacagattta
    18481 gtgtatgctc ttgttggctc tacaatagct aagaatactc caattgtgtt agttgatgac
    18541 aatagtaata agactgcgtt aaaaggtgca actaaaataa cagcaatagg aaatctaaat
    18601 aacaatgcta tagaacaatc tctaaacata acaaaaaata ttgccagtcc aactactgga
    18661 gggaagtata ctgtaaagga agcaactgaa agaatagctt atattttaga tgaaaaagta
    18721 tttgtagatt atgaatcagc tattaaagca aaaacagacc cagatgaatg ttttaggtac
    18781 tatataaaaa actttaccta tcaagggaaa gattattatg caattggcgc agatgatgat
    18841 gattttagat ttttagtaaa tatgaataat ttgaatgata tactacaatg gtattcaaat
    18901 gatacaataa aaaggtttga tgctgaagga tatttaaaag agagaaaagc aatcgatatg
    18961 gcatttgagg catgctctag taaatatggt attagtgaag atgatttgat actagataga
    19021 gaagaacata atataagcac tgatttttat gtggttgtat tgtatgagag ttctggaata
    19081 gattcatatt gggtttttat agatactgat acatggactg ttcatgatat acagcgttta
    19141 aatcaaaatg aagaattagt atcatatcat gatgtaaacc caaatacaga agcagataag
    19201 cattataata gagatgttga tgacgttaat attatatttg ttccatcaga aaataatcct
    19261 tttgataatg atgtagatgg aataaattaa atatttatta taaggagtta aactataaat
    19321 tgatataaat atttaaaaat aatgtattta tgatataata aaactatgga aattttgcag
    19381 tgagcgatat ttatgataaa atggggctta gcagttgaaa tataaggtat tgagggtgta
    19441 tgataagtgt tatcatttgc actactgccc gctcactgca aatttaagag agttgtgtat
    19501 gcgtaggcat tgaaaatact caacttattt tggggtttta tattaactat gtggtatgta
    19561 aataggtaaa ctaaaaggaa atttgttttt cacattttgc atgttttata ttaactatgt
    19621 ggtatgtaaa tattttaggt tcaagtttta aaattgattt atctataagt tttatattaa
    19681 ctatgtggta tgtaaattta tctcatgcgc aaaggcaaat acattgtcta ctggttttat
    19741 attaactatg tggtatgtaa atcattaata ttataac
//
LOCUS       DAEMHZ010000064        19585 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000064 DAEMHZ010000000
VERSION     DAEMHZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.064"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            185..1588
                     /locus_tag="KPK72_002188"
     CDS             185..1588
                     /locus_tag="KPK72_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2489400.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2027..3049)
                     /locus_tag="KPK72_002189"
     CDS             complement(2027..3049)
                     /locus_tag="KPK72_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2489401.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3084..4682)
                     /locus_tag="KPK72_002190"
     CDS             complement(3084..4682)
                     /locus_tag="KPK72_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2489402.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNVDDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4724..5989)
                     /locus_tag="KPK72_002191"
     CDS             complement(4724..5989)
                     /locus_tag="KPK72_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2489403.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6133..7545)
                     /locus_tag="KPK72_002192"
     CDS             complement(6133..7545)
                     /locus_tag="KPK72_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG2489404.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGPESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            complement(7583..8590)
                     /locus_tag="KPK72_002193"
     CDS             complement(7583..8590)
                     /locus_tag="KPK72_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2489405.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEIENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKSLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESAAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDIIERTKSIFN
                     A"
     gene            complement(8787..10109)
                     /locus_tag="KPK72_002194"
     CDS             complement(8787..10109)
                     /locus_tag="KPK72_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489406.1"
                     /translation="MKILYVGPGMVIDKILSVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10468..12108)
                     /locus_tag="KPK72_002195"
     CDS             complement(10468..12108)
                     /locus_tag="KPK72_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG2489407.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12264..12920)
                     /locus_tag="KPK72_002196"
     CDS             complement(12264..12920)
                     /locus_tag="KPK72_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489408.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            complement(12946..13647)
                     /locus_tag="KPK72_002197"
     CDS             complement(12946..13647)
                     /locus_tag="KPK72_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG2489409.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            14453..14932
                     /locus_tag="KPK72_002198"
     CDS             14453..14932
                     /locus_tag="KPK72_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2489410.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            14929..15708
                     /locus_tag="KPK72_002199"
     CDS             14929..15708
                     /locus_tag="KPK72_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489411.1"
                     /translation="MNKNILNNSFPEVPESFHNSLNKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSIKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(15958..16458)
                     /locus_tag="KPK72_002200"
     CDS             complement(15958..16458)
                     /locus_tag="KPK72_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2489412.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVLKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(17783..19510)
                     /locus_tag="KPK72_002201"
     CDS             complement(17783..19510)
                     /locus_tag="KPK72_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489413.1"
                     /translation="MENKNLIRFSKLVIHRNRVNLIFSFIFVCIISIIDLFLPQITRL
                     IIDEGINNENITLLIKLIILYGLIKVLSSLFNLILSYLYSLMKNKVTISLKIKLLNHV
                     SKLSGRYYSNMKTGNTLSIIENDIFVLKNFGAELIFSILENLITAIVAIFFLMKIRYD
                     LFLVVLILQVSLALIQTKITKIISKDTKEVREESGIISNITQEYISNIMSIVITKSKL
                     KFFISYLRKERNLARKFLRLDMIMAGNISASQILNGFVTMSVYGFGGYNIINKKMTIG
                     ELIAFQQYTSFLIGPCISIIRSINRIQQSKVSIDRVYSIIDEEIEIKVNNMGHIINLK
                     NCNSIKLSNVYFKYYNDSDYVLDNISMEFRKGEITALVGGSGCGKSTIINLIYRLWDV
                     TDGNIFIDNINIKDINLKSLRDNITIVTQDLLIFDDTIKNNIIINNNVSDDILNEICK
                     IVGLNDYINTLDNGLDTIVGEKGTKISGGQKQRIALARALISNNNILILDEATSSLDN
                     ISQNAILNNISKYIKDKIVIIIAHRLSTIKSANNIYVLEKGTVIESGNNKELFEANGM
                     YKKLVKAIN"
BASE COUNT         6788 a         3384 c         2254 g         7159 t
ORIGIN      
        1 aatactttat tcaatacata agtagaaatc tctatataca taaattcatt ttttcataca
       61 tcttacaaat ttatttttat ttagataaca tgtatatttt ttccatgaaa aataaataat
      121 aaaaaatagt aaatatggaa aatataaaat tgattaacaa taattaatta cctgaggtgt
      181 gaatatgaaa atgctaaaca acttttttaa gaaaatcaac aatccagctc ctgtctttgc
      241 acagacaaaa atggaaagag ataatcaaac agatgattca ctttctacaa aaccaaagac
      301 tacatttaga gacgttgctg gattagacga agtaaaagaa gaactatttg aaatagtcga
      361 ttttatgaag tcaccacaaa aatatcaaaa aatgggtgca aaaataccta aaggagtatt
      421 attttatggt cctccaggaa caggtaaaac cctacttgca tctgctgtag ctggtgaaac
      481 aaactcatca tttttcaatg ttaccggttc agagttcgtt gaaaaatatg taggtgtggg
      541 agcaaaaaga gttagaactc tatttgaaaa agctagaaaa gaagctccaa gtataatatt
      601 tatagacgaa atagatgctg taggagcaaa gagacatctt gaaagtaaca acgaaaaaga
      661 ccaaacacta aaccaacttt tagttgaaat ggatggattt aataaagatt ccaatgtatt
      721 aataataggt gctaccaata gacttgactt attagatgaa gctttactta gaccaggaag
      781 atttgacaga cacattcata taggtgcacc aaactatcac actagatttg aaattctaaa
      841 ggtacataca gatgacaaac ctatagacaa atctgtaaat ctagaacttt tagctaaaaa
      901 aactcatggt ttcaacgggg ctcatctttc aaatatagca aatgaagcag ctatatttgc
      961 agttagagat gacagtgagt gtattacatc agaacatttt gacaaggcac ttgaaagagt
     1021 tattgcaggg ctagaatcta aaaactctgc attggttgaa aaagagaaaa aaatagtagc
     1081 atatcatgaa gctggacatg ctcttgtaag tgatatagta ggaatttgtc ctatacaaaa
     1141 aatatctata gttcctagag gacaagcatt aggttatgtt cttcaattac ctgatgaaga
     1201 tagatatatt tatactaagg atgagttgat tggaaaaata aaaatattac ttgctggaaa
     1261 ggcttcagaa gaattaatat ttaatcataa atctactggt gcaaaggacg acttaaagaa
     1321 agtcactgaa atagctaacc aaatggtttg tgaatatggt atgagtaact taggatttat
     1381 gactattgat ggaaatgata agactttctt atgtgataag gttcaaaagg aagcaaatcg
     1441 tatagttgaa atttgttata aagagacttt agagatgtta aaggataatc ttgaagattt
     1501 acattctgtt tctaaattct tatttgaaaa agaaactatg actcatgaag agttaaaaga
     1561 tttgatagga aaagaagctg taaactaata tagttttaaa aatttgaggc tgtctcaaaa
     1621 tagagataag cctcttttca ttgttaaagt tataaaaata catttgattt tatacaaaaa
     1681 agtgcctcat aaactaaaaa tttcattttg agacacttac atattttaat tacatatttt
     1741 aattttgtct atttacagaa tattatatct acataaaact aaactgctgt aaacttaact
     1801 acctccagta atgtttgaag tggtataaaa tctagtagtc tagtttctac acttataatt
     1861 tatattttac ataactaaat ttagtagatt tttatactca caatttagaa cgattgaaaa
     1921 cttaaaaatt tcttatcaga tatctacaac ttaattaggt atctatcact tgattaaata
     1981 tctgccactt aattaaatat ctaccactta attaaatatc tatcacttaa agtgtagaac
     2041 ctctttcaat caattcaggt tttaacattt caacaactga ctctggattt tcttcattta
     2101 ataaggactt aagtatgtta attgtttttt caatcaacaa atctagtggc tgtctaatcg
     2161 tcgttaaatt ataactcttc catgaagcca tttgaatatc atcatatcct atcactgata
     2221 aatcctctgg caccctcaac ttgtactcaa accttgcagc atccattaca ccaatagcta
     2281 tcaagtcact tgtgcaaaaa attgctgttg gaggatttga taatgctaac attctcttac
     2341 ctgcttcata tccagaatca taatcatagt ttgcagattc ttctttaagg ttatgaatcc
     2401 cactttgtct taacttagaa taaaatccaa ttttcttttc catttcttca cttgtttctc
     2461 tcgtaaattg aatatatcct atattcttat gacctttctt aagcagatat tcagcagcca
     2521 ttcccccacc ttcaattggg tctacataga ctgtacttat ttttatacca ggtataaaac
     2581 gattaaaaag aatcacagga atattgtttt cttcacaagt ctcagccatc accttattca
     2641 tggccgaagc tgtaataatc acagcttcta cttgaaactg aattactttt gaaattatac
     2701 tatctatact ttgttgtcta ggaactttaa aaacgagaca ctgtttacct atctcttgaa
     2761 ttttttctac aaataaattg ataattctat tgtaaaatgg acctagacta tcaccaacta
     2821 tcaatcctat aacattagtt tttccactaa tcattccccg tgctaccaaa ttaggaacat
     2881 atcctatttc atttgctaca cgaaaaattc tttctcttac ttcaggtttt acgccctttc
     2941 catttgcact accaaatgct cttgaaacag atgattgaga tactcctgcc aatcttgcaa
     3001 catcttttga ggtagccgat ttattataat gaactttctt ttgattcatt tagtatttct
     3061 ccttaaatat ctagtatttc ttcttaaaat tgtatcaaaa ataatataat atattggata
     3121 ataaaaaaag ctaatacaat tagttcttgt ttacctaaag aaaatggcac tatagatact
     3181 ccatctactt tatccattcc ttgagaaata aggtgatttc tcataccaaa tgcagtgtat
     3241 tttttaatat catctacatt atgatttttc tttaaaatga cactaaaaat atatccaaat
     3301 atcaatgtta ttgtagcacc tattgctgga ttccacaacc aactcacatt atatttatta
     3361 gaaataaaaa atcctaaaac taaccctaca ataaaaccaa tagatactcc tttatcatta
     3421 gctttatatg taaacattgc tagtacaaat gcacctgtag ctggtccagc aaagtatgag
     3481 atatagttac caacaaggtc aagaattgat ttgatgcttc ctccaaatcc cataataata
     3541 aaacctgtca taacaactcc tataactaca gttataatca ttgtaacttt taatgatgat
     3601 tctttgttgt cttttttgaa ataaaactca taaatatcct tcgtaaaaac agttgtcata
     3661 ctgtttaata tagagtcgat actagacatt gctgctgcaa acacacctgc aataataatt
     3721 ccaatggctc ctactggaag ttcatttaaa ataaaagtaa tcattatttc atttgaagtt
     3781 tcaaatgtcc ttcccccata aaaaacaaat aaaattactc ctaccaatag cataaagtag
     3841 taaactaaat tcataacaaa tgcactaact acaaaagaat tttttgcaca ttttaaagat
     3901 ttagatgtta aaactctttg tacttgagat tggtcaaaac aaaagtatct tatccacatt
     3961 acagttccac caattaaagt agcccaaaaa gtatttgtca ctgaaatatc cctactgaaa
     4021 tcaaatgtat ttaattttcc actctgttgt gcaatttgta atgtatcaaa gaaacttaga
     4081 ttcattcctt ttaaggctac aataattact ataaatacag cacttattac tactatcatt
     4141 tgaatactat cagtccatat aactgcctta atcccaccta atagagtata tataattgat
     4201 acaagtacaa ctattgggac taaagcataa agtgaccagc cagtaatcat ttgaataata
     4261 agcactggaa tatataccat agaactagct tgaatcaatg aattaataaa aaattgaata
     4321 atagttaagc ctcttgaata atttcctaaa cgaagtccca tatattcata tatagaggtt
     4381 atttttaatt tatagataac tggggtggta atgctcagtg caaaaaatat tgctaaaggt
     4441 actgtaatat taaccattac tggatacatt ccatctacat atgcccatcc aggaccacct
     4501 ataaagccat ttgcactaat catagtagct gcaactgata aagcaattgg aatccatggc
     4561 attgacctac ctgccaagaa ataatcttct tgatttttat tatcttttcc tgaataataa
     4621 ccaataacta acattaatag caaatatcct attataatac atacatcaat tgatgaaatc
     4681 ataagccacc cctttctaag taacaaaata aattcctagt attttatttt ttattattta
     4741 acgtctctgg taagaataac catacaatag cacctgctac aaagactgcg attgatatgt
     4801 aaaagatagt catcatacct aaagtaccag aagctgtacc agctatagca ggtattgcaa
     4861 acgtagatat aatccttcct ccattaaatg acataccaga tgacaacgaa cgatatttac
     4921 ttgggaacaa ctctgtgtag tatgctcccc atgctccaga atatccaaca gcaacaccta
     4981 gtaatactga tatcatatag aatgttgttg tattatctcc acctaatttt gtatatataa
     5041 aaacaaatac tgctgctaat gccataccta taaaaggtat ttttcttcca aatttatctg
     5101 caattgaacc ccagaataaa tatcctacta attgaccaat tccttgtaaa ctatatatag
     5161 acccagccac tgctgctgac attcctattc ctgtcactag gtatgtagtt gcattatttg
     5221 aaaatgctga atatgctgta aaatttgcac ctgataaaag aatcgttccc aatccaatcc
     5281 ataagtattt tcccttatac atacttatat agctaatctt ttcaccagtt tcttttccct
     5341 ttttaactaa ttcctttgta tactccaaat catctggcac aaagaagatt gctaagatag
     5401 ctccaactat aggtggtata atcgcagtat acatcaatat ttgccaattt gcatcaccta
     5461 aaacgccata tactttccca ccaaaaatca ttgctaatga aaataaagca gttaatatag
     5521 cagctaactt accacgatat ttacttggaa acatatctgc aaccaaaggc atagctgttc
     5581 caaatacgcc tcccaacact actcctgtac acaaacgtgc tattttccat aacataatat
     5641 ttgtaggaga taacattggt ataaatgttg tcaatccaat cagtaaagca acgctaatta
     5701 aagtcctctt cttcccaatt tttgtagaaa tcaccccaaa aataaatgta cctggaattg
     5761 taccaaaaga cataactgca aatatttctt ctgtttgttt taatgaaaaa ccaaatcccc
     5821 catctgctaa agcataaatc attgttggtt taataaaagt acccaccata aaattgtagc
     5881 aataaaaaaa gtatattaaa gcaattgaaa aaaatgcaat tcctcttttt aggtttgaat
     5941 agtcttttcc taatatgata tcattttcta tttcaaccgt tgttttcata tttttataat
     6001 tccccctttt aaatttagtc ataattttgc taaaagttta aacatctatt tttcatatac
     6061 attccccact taaattttat aaagcaatac ttccctatat acatttcaaa actatttata
     6121 ctctaaacag ctttattctt ctactcccat atacatttgt tttaatacat ccataccatc
     6181 aaaagttatc cactcctcct ttactttacc ttgtagtata tgataatgat tgattcccat
     6241 tacttctata tgctttccag aaggctgtcc aaaaaatcct ataccttcat ttattccacg
     6301 taatctccat cttacagata catcataacc ttcatttttt tctctttgat tacaagttac
     6361 tctttccact tcataacttc catttggaat tgatgcaagt aaacttacaa tcattccttg
     6421 aatctcatca taaccattta aatctttatc acatataaaa tgtactactg cattatcatg
     6481 atagtatttt ttaacttcat ttatatattt ataattataa atacggcttg tcatttcaag
     6541 catcatttcg ccaacagaat catcttttgc ttgatactta gttggcataa attgaccatc
     6601 catagattca cagataccat aagttgattg taaactaagc actttagatt cacttgcctt
     6661 tgccatgctc tttgctactt cctgagggtt taatcctaat tgagtaacaa tccataaatt
     6721 atctctaacc aaccattctt cgtggattct attgttagtt gtggcacaat caattacagt
     6781 tctaaagttt atttttctac ctgttgctgg tccaaaatta ctatcaccta agttagttgc
     6841 tgtagacaaa acacggtgag aagataaaaa accatcagca ccaaagttag accatattac
     6901 attttgacca attaatcttc tatcaggaaa cgcatgcaat gtttgaagag tattggaaat
     6961 aacatcttta ataccaacta aatttgaaga gccacaatgc attgtaacat tattgtgata
     7021 tgtatcataa ataacaccta tacctttttc ttcccagata ttatgtgtaa tcttcataat
     7081 ataatctaca aaatctctat acttagaatc aaatccaggt atttcctgtt ccctttttgt
     7141 ctgctttgaa aaatcattgt aatccattac tccaacaggc gttacttcta atgaatctcc
     7201 atagtaaatt tttgtagatg acatattatc ttccccctta aatataactt ttttattttt
     7261 ttcagtaaga gtatttgtat ttaatttatc tgtatccact gtatctatgt taactttatt
     7321 tatgtcggat ttatttgtgt ttgttttatc tatattccct ttatctatat ttcctttata
     7381 tgtgttcgtt ttattctcat ttgtttgatt ttcatttata ttactaaaat tagtttgatt
     7441 cattttagct ttgttttctt taaccgtaca tgattcaggc ccacttgtag aaacttcttt
     7501 ttcttcctct ttacttgtta cattattttt gttggtagat gccactttat atcccccttt
     7561 gttttagaat acgtatgcat aactaagcat taaaaatact tttagttctc tcaataatat
     7621 caagcccttg ttgtttcaac caataaggaa tatcaattaa cacccagttt tcttgaagtt
     7681 tatcgccctt acgataataa acatcaacaa cttgcatatc tgctttaacc tctccacctg
     7741 gtaatcctaa aaaacctcca catggtacat tagtcagatt aggccatcca aagaaacaag
     7801 caaaattacc ttctgcaaat ctacatacat gcccattgaa agtctttcct tttaaattat
     7861 ttctaaatgg tagctggtgt tgctcttgat atctaggtat agtataagaa gcaccaattc
     7921 cacctggacc ataccaaatc atatctttag cccaagattt ttcaagtatt tctggtgggc
     7981 atcccatagc agcactttca tttaattcag ataaatcatc taccatttta tttactaatg
     8041 ctaaagtttt tactccttca acttcatctg catcttcaaa aagtaatcca ttatgatcaa
     8101 ttggtcctgg atatacaaaa tatgaacctg tttgtggtgg caatggatta actcctgctt
     8161 gaatcataaa cccaattata tctacaaata atccagtttt agcaatttta ccatcttgaa
     8221 cacagttaaa ttctgcatat cttaaactaa tcattttacg agtatgttta atgccaagcc
     8281 aatccttatc aaaaagtccc ataaaatgcc ccatactcat tacccattca ctattgtcaa
     8341 tctcatttgt tcctgcaatg aaaatatctt gtcgtctttg catattactc aatgattttt
     8401 ttaaagactt ccaaaataca tctgctacat tttcagtgcc ttcttgttca cgaaatggat
     8461 acacaccttt ccagttataa tctgatttca tatgttgctt taagacattt tcaatctcat
     8521 ccccagtagc attttctaat gcttcaaaat aatttcttac aacttttttt gaatcctgat
     8581 attttcccat aattatctcc tcccagattt atttaagtta taaattaact ttctcatttt
     8641 atgcatacga tttctttttt aataattttt tagctgtttt gagtttattt tatgaataca
     8701 tatgcatttt tctaaatatt aacatatatc taaaaaattg taaagaaaat gttatcataa
     8761 aaatttaatt tttgacttat atctatttaa aacaacagaa aaggtttaaa acgtaatatt
     8821 ctactgggtc taccatactt gctcataagc tgttcactca ctacctcaca ataaccagaa
     8881 tcacacaatc taagtataat cctgtccata gttctaatac ttacaccaca tataatagct
     8941 aattcctttg atgtaaattc agtttttcca ctatttatta ttttattgaa cactttatat
     9001 acagtattag cacttagccc tgtctctgta gcaatttgat aaaatcgttc atcaaagcta
     9061 tttttctctt tgacagcagg atttggcttt agtggtccca taatttctcc attttcaaaa
     9121 actacatatg ctgcatcatt tttttgactt tgagccatct taatactctc ataagcgtta
     9181 aatttagctt catttgctgt ttttccataa ccaattccta tataagtgtt tataactgaa
     9241 acttcacgaa tcatatccaa caaataaata tctctgtact tctttgtttc taactcaagt
     9301 atttttttag ttgtaaaaat cataaattcg cttctactct gctctaccaa aacaccatct
     9361 atacggctat tgaaatggta tagtttatca agaatcttaa tcctttgtga aagataagcg
     9421 tactcatctt ccttcatcag tgaatattct gaaggcatat taagtttgat agcaagaaca
     9481 acaatctgat tctcattgtt tttctttgca atataacgca tctgaatgtt ttttatactt
     9541 tgtaccatta catcctcaga aggcaaggct aaaactactg gaatattctc ttttttcatt
     9601 gcatgatatg cttcttcaag acctgtaata caacaacata tgtcattatt ttgataatta
     9661 tatttatgaa attcaagcac aaactttatg tagtcctctt ctaaatactt ttgctctgca
     9721 aaaaacatat gttcatcctt aaattcaact ccaatttctt tatacaatct cttcagcata
     9781 gctttatcat aagtatctat actaagtcta ctaatatcga atttaagcaa atatgtaatt
     9841 tcaagcaata ctctgtataa tgttgtttca tgtctaggaa tataatcaga caacacaaaa
     9901 tctttttgtc ttttttcaaa aaatttaaat ggcgatttac ctgtaaaaag aactgcatct
     9961 actctacctc tatatttttc caataaatca ggtacttcaa tatatctatc atatctaaga
    10021 aaatctgctt caatattggg aaaattcttt ttacttactg atagtatttt gtcgattacc
    10081 atacctggtc ctacataaag tattttcatt tcgtttctcc tttcctaaag atactattat
    10141 ctcttaagac actttctgaa cctcccttga cttaaagtaa caaggttgct gcttcataaa
    10201 attttgcata atcaattata tcttatataa aaagttactt taagtttttt aattatgact
    10261 atctgttaaa acatttttgt atttaagttt ttgtatttaa atcttatcca ttaatattaa
    10321 ttacatttta caattaatat attgtttttt ctacttaaag ttaaattctc tagaaaacac
    10381 aagtagagta atctttatta atctaacaaa aatagccttc cttaatattc aaagaaagac
    10441 tatttttata aaagttctta atgtattcta ttttgctatg ctaagaggat ttttaattac
    10501 acgtccagca catacatcag tttgctttcc tcctgccaat gcaattttac catttaccaa
    10561 tacatattcc actcccactg tatactgttt aggattacta taagtcgctt tatcttctat
    10621 tgtatctggg tcaaatacca caatgtcagc aaaataattt tccttaagta gacccctttc
    10681 tttataaatt cctaagcgtt ttgctgaaag ataagttatc tttttaactg cttcctcaaa
    10741 agtcaacatc cctttttctc taacatagta agagaaaatt cgtccaaaag taccaaatgc
    10801 acggggatga ggctttccaa gagaaagtaa accttctgta gaaaggtcca tactatcaga
    10861 acctatacat gactttggat ggcaaacgac cttatctgta tcctcttgac cttgtgtatg
    10921 aacaactact tgaattttac ttttttcttc aatcaaaagc ctaaagaacg tatcaaatgg
    10981 attttcacca ttgattttag ctacctccat taaagtttta ccctctacat atttatttgc
    11041 ttcagtcatt acagaggcta gtacaacacc gtcccaacca cctgctaact ggtagaaaca
    11101 ttgccatcca ggaatccctt cctctagttc ttttataatt ctgtccctat tttcttgcac
    11161 ttctaatcgt tttaccatgt tttctacacc accttcaaaa gcccaaggag gtaaaagtgc
    11221 acccaaagat gtcattccag ctgtatatgg gtaacaatca aaagtaactt ccaagccatc
    11281 tgcctcagct tcatctagct ttttaaatac cttatcaata gttccaaaat tcttatgacc
    11341 catgactttt aaatgagaaa tatgtagagg aactcctgcc gtcttagcta tataaattgc
    11401 ttcatcaaga gcttccattt cttcatcacc ctcatctcta atatgagata tatagtatcc
    11461 attgtatggt atcaattcct tacataattc agccatttct tctttctttg tatatgctcc
    11521 tggtaaataa actaatcctg acgaaagagc tacacatcca gcttccatag cctttctaac
    11581 ttccaacttc atatttttaa gttgctctaa agtagctggc tcctttgaaa atcccatttc
    11641 attgatacgt acaggtcctt gtgctacata agctacaaca tttactgctg gagaattttc
    11701 agttaagtaa tcaaagtatt cctgcattgt attccagtga aggttcaatt ttattggaat
    11761 tgtaccaatt atcctcgttc caagatagtc aattaactcc tgcttcttct cttctcttat
    11821 cggagcaact cctataccac agtttgcaat aacgtctgtt gttacacctg tatggatacg
    11881 gcttcttgtg tatttgtcat acacaagaga taaatcagaa tgagtgtgga aatctataaa
    11941 accaggtgat acaaattttc cagttgcgtc tatagtctct ttagcctctt tttgacttag
    12001 atctccaata gccatgattt tgtctcctac aatccctaca tcacctttat atcttgaatt
    12061 tcctgtacca tcaatgatta ttccattggc aattttaaca tcaaacattc ttcttctcct
    12121 ttcaagactt atttatattc atatttctta ataaacaatc ctattctttt ttctcaacaa
    12181 ataattctag tctttcctcc tagtaaacaa tcctattctt tttctcagca aataattcta
    12241 gtctttcttc ctagtaaaca atcctattct ttcgttttat tgctatcaat tacaaagaaa
    12301 acccttgtaa taagtatccc acctaacaca ataaacaatg tcaacaatcc acctggtata
    12361 ccaatttttt caccaaagta cataatagct aatgctacag caatcgtaca tccaccagct
    12421 cgaatatctt tacgcgcaat atcaacgtaa attgccccaa acaatgctgg aagtatataa
    12481 ttaaatgaat cagtaataaa ttcaggtaac aaaggaatta ctttatagcc tacaatggta
    12541 aaaatagtaa tcatagaagc tgatacaaag attgaacaac atgtaccaat tgttcctaca
    12601 acttcaccct catgagttcc tgcttctaca cctgcaattt tctgtgccat tgatgctgca
    12661 ggcatacgta tatctccaac agaacctgca agccaaccta tataagaacc tggagctcct
    12721 agtgttggat aataagctat aggttgaaca atccaactca ctccataagt tgtagcaact
    12781 aatccccaaa tttggaatat aacagaaagc attggcatct gaccaaagcg aagacctaca
    12841 tagattgcag gaataaaatt tgcaataatt gcaagaccta tagaaattct ccctattttt
    12901 atcatctttt tatcatacat aattatcacc tcttatctgt tttaatcatg atagtacaat
    12961 ctgtgtgcca aacataccaa tcaacatggc aatacctaag gataattctt gtaatcgttg
    13021 attcttttta aataaagtcg tcaatactag cataactata gctgaaataa ctgctggtgt
    13081 aagttttggt atttcgaatg tttttatttg atttgaaaga aggtaagcaa ataatccaac
    13141 tgtagcccct gcaagtagtt ttttaaccca agtaggatta tatttcacat tcattttttc
    13201 aacaattcca cgcattctat gacccaaaat aaatacaaca agcaaccaac ctaagttatt
    13261 taaagccatt ccccatagtg catacgaaaa agcttctgct ccaaatgcag cactacctac
    13321 ttctactcca gataaatttg atgtaagaga tattactcca agttctgttc tggcagctcc
    13381 tacatcacaa agcctcatcc aagtcgtagg agcaccaatc acagctacca gtgataataa
    13441 tgtaataaca ggagatagag agggtccaat tgctgtaaca ctagctgagc gaattgctgc
    13501 tttaattcct tttctttcaa taccaagatt tgaagcctct ttaagtgctg atttcataaa
    13561 tacgattgct tgaacaactg aaataattac taaaaaacta gaaacaatcc aaagacctgg
    13621 gctattaatc atttcttgaa acttcatact ttattcctcc ttaaaataaa ttttttagtg
    13681 ttttattatg agctttcttt ctgtaatctt gttatgtttt tatattgttt taatttattt
    13741 tatctaagat atagtatctt taataggaga ttatctgtct taaagtctca ctaaaaacat
    13801 taatacttaa atactttatt gataattagt aaatatcaga aaattcacat attatttttt
    13861 taacctgtaa ttaacttata aggtatttta ttactttgac atttcaatat aaaactttta
    13921 atcaaaaaac tttgttattt taaaaaaatt attattttta taatttatat tttttaagtt
    13981 tatttatgtt catctctatc taaaataatg acatttctaa ctcttgattc tcatttaagt
    14041 ttcattgttt tttcttaaaa gcctactttt agtcaaaata ccgttatctt tagtcattaa
    14101 ttaaaaaact tattttaaat ctatacatga agtaaataat ccaaaagcat tattttctaa
    14161 ctactcaaca tcaaatactt actattttat aattacttaa tataaacact tactataaaa
    14221 ctaaatatat aaatggcttc taaagtttaa tatatctaca tggctttata tatgagggga
    14281 aaatactaga aaaaaatata ttatagaata tttgtataga ataggcaaaa ctttcaaact
    14341 taaatttatc aaataatcat aaaaaaagtg aaccatctat agtaaaattt tatctaataa
    14401 gtgaaaggca aaaaagtagc gctacgattt gcatagaagg gaaggaactc agttggataa
    14461 aactacattt acaaacaata tacttgaatc agaacagact ttgtatcgag tatctaagtc
    14521 tattctagga aacgaccaag actgtgaaga tgccgtcaac aatgctattt taaaagcata
    14581 tgaaaagttg gattctctta aggaagaaca atactttaaa acatggctga ttagaatagt
    14641 cataaatgaa tgtaattcat taagacgcaa acgattaaaa tctctatcat ttgaagatgt
    14701 cttcaaaaac aaaaaaattg atgaaaaaga tgactatagc gatttgtata ctgctattca
    14761 aagtctacct aaaaaaatta aaattccaat agtcctttac tatattgaag gttattcagt
    14821 agatgaagtt aaggaaatat tagatatacc tcaaggaact gtaaaaagta gattatcaag
    14881 aggaagaaca ttattaaaaa ctaaattaga aaacacggag gtaatcatat gaataaaaat
    14941 atattaaata attcgtttcc agaagttcca gaaagttttc ataatagttt aaacaaaaca
    15001 ctaaatagtt taccagaaag agaggaaaat tataaaatga ttaataataa aacacacaaa
    15061 ttaccattta gaaaaggttt agttgcaact ttagccatta ctttgatatt gagcacaaca
    15121 gcccttgcag taggacatat atatagttta gttggaaaat caagtattaa ggctacctat
    15181 acatctatcc caactacaga acaagtaaaa aaagacttta actttacccc taatatagtt
    15241 tctgaattta gtaatggata taaatttaaa ggagcatata ctgtaaataa aaaagcactt
    15301 gacaaagaaa ataatgtttt aggaaaatca aaagcactta gttttgaata taaaaatggc
    15361 aatgatgaaa tatctcttga cacagcaaat aatgtacttg ggaagagttc tgacaatcta
    15421 aaagttgtag atacttacaa agatattaat atttcttatg aagcattcgc acaaaagttt
    15481 gtaccagaag gttataaaat gacagaacag gataaaattg atgaaaaatc aggcaaatac
    15541 acatttagtg tagggccaaa taatgaatca gacaaagttg aagttaatga atttaagttt
    15601 ttgacatgga aacaagatgg agtttactat tcttttacag ttcaagactc aaatttatct
    15661 atgaatgact tagttaaaat ggctcatgaa gtaatagatg caaaataaac ataagtaata
    15721 ataagttgtt tatgagttta ttatttatga ctttaatttt tttattagta aacatggttg
    15781 tatcttccat ctttatattt tctttaaatt taatatataa aattttagat atttagctac
    15841 cttcaaaaga accattatta aaaagccctt aaaaatattt cttttatatg gaattttaag
    15901 ggctttaact ttattttata caagtgtata tggaatttta aagactttaa ctttatttta
    15961 tataagtgta agctccattt gaattacttc ttcttttaca ggtctatcat tccatgtaaa
    16021 accactagat attgtattta cctctacaaa ccctaaagat ttataaacat gaatagctct
    16081 actattactt ttcaatacat caagctcaat tttttcatac ccatattctt ttgataaatc
    16141 aataatttgt tttaaaagac tacttccgat acctttttcc ctatactcct taagaatacc
    16201 catgcctaaa taaccaactt tttcagtttt aggtaaaacg tcacaccaac caatacagtt
    16261 atctgattct aaatcaatta taaacagttg tggtaagttt ttatttatag tattttttat
    16321 aaattcaact gtttcttcaa aaggaaaggc ttcatccata gctagatatt tcctctcttt
    16381 tgctatttta tcaaaagttt gccaatacga ataaatatac ttttcttctg cctttataaa
    16441 ttttattcct tctatcaact taacatttcc cctttttaat ttgtgattgt agttaactta
    16501 ttttatatga ttctagttta taatgctata taaattacta tatattagct taaaatatct
    16561 ttgtatattt ctataaaatt aaaaccaata tatctatctc ttgtatctct attaaaataa
    16621 tttttaatag tataataaat atagttcttt aatcttttgg gtatatatac ttaatcattt
    16681 tgaaatttga tttaaagtat aataaaatat ataaaaaaat atataaaatg gtactataaa
    16741 atttttaatc ttcatcaata ccttttttgc tttcgttgtt aaaactattt tttctattag
    16801 tttatttctt tgtaaagaat tttaggtact gtttcatcaa tcattcaata aactttggat
    16861 gttgctttac catcggttca aagatttttt ctttattaaa aaacttgata gatttctttg
    16921 tcagtaaaac cgcctaaata ttatttatga tgtgatattg atggttatat atgtgctcta
    16981 tcaaaattaa cactatacta actttttcta gctcatcagt aatttttgta agaatcttac
    17041 ttaaactagt cttttcttct acttctaata cgttaaatat ctgattactt gagtattatt
    17101 tctgttaatt tttgttaatt taatccaaag cctttaatat caattttact accattactt
    17161 tatgagcgta tacaagtaat aaattcttgc ttttctagtt ttgaaagtga ttcatctaga
    17221 gattgatagg ttttatatca aataagtacg atagttcttt attaagcaat tttagacttt
    17281 atttttagta tatataatgc tctggctttc cttcttcatg ttgttctaaa attcaatatg
    17341 attttgaggg taaaatttat ataccaatga ttgtacttaa aaaaattatt ctaataaatt
    17401 atcctctttt acttcattta ttatttttat cagtgcctaa accttataaa taaaaaatat
    17461 attctactac taaatgtttc tctataaaac tgtatttagg ttcatttata aattcacaaa
    17521 aaacttactt tttaaatgat tttttataaa ttaaatttta taatgttgct ggttttgcat
    17581 cctccttttt ttatgtcttt aacagttgtg tgaaagatat tatattttaa tctaaatagt
    17641 ctttttttat attcttaatt gcataaattt atatttataa cattgtatta ttttatttat
    17701 acaattttgt ataaaaataa attaaataca ttttatacta agtttttgga tacacattct
    17761 caaaatacaa tcttgaatag atttaattta ttgctttaac tagcttttta tacattccat
    17821 tagcttcaaa caattcttta ttgttccctg attctataac agtacctttt tctaaaacat
    17881 atatattatt agcacttttt atagttgaaa gtctatgtgc tattataatc actattttat
    17941 cttttatata tttacttata ttatttaaaa tagcattttg tgatatatta tcaagtgaag
    18001 atgttgcttc atctaaaatt aatatattat tattgctaat taatgctcta gctagggcaa
    18061 ttcgttgttt ttgaccacct gaaatcttag tacctttttc tccaactatt gtatctaatc
    18121 cattatctag ggtatttata taatcattta atcctactat cttacatatc tcatttaata
    18181 tgtcatcact aacattatta tttataatta tattattttt aatagtatca tcaaatataa
    18241 gtaaatcttg tgtgactatt gttatattat cacgtaatga ttttagattt atatctttaa
    18301 tatttatatt atcaataaat atatttccat ctgttacatc ccaaagtcta tatatcaaat
    18361 taataattgt tgatttgcca catccactgc ctcctaccaa tgctgtaatt tctccttttc
    18421 taaactccat acttatatta tctaatacat aatcactatc attataatat ttgaaataca
    18481 cattagaaag ctttatagaa ttacaatttt ttaaattaat tatatgaccc atgttattta
    18541 cttttatttc tatttcttca tctattatag aataaactct gtcaatagat acttttgatt
    18601 gttgtattct atttattgat ctaataatac ttatgcaagg cccaattagg aaacttgtat
    18661 actgctgaaa tgcaattaat tcccctattg tcattttttt atttattata ttataacccc
    18721 caaatccata aactgacata gtaacaaacc catttaatat ttgagatgca gatatatttc
    18781 cagccattat catatctaat ctcaaaaatt ttctagctaa atttctttcc ttccttagat
    18841 atgatataaa aaatttcaat ttagacttag ttattactat actcataata tttgatatgt
    18901 attcttgtgt aatatttgat attattccag attcttctcg aacttctttt gtgtcttttg
    18961 atataatttt tgtaatctta gtttgaatta aagctagact aacttgcaag atgagaacaa
    19021 ctagaaataa atcataacgt attttcatta aaaaaaatat agcaactata gctgtaatta
    19081 gattttctaa aatagaaaat attaattctg caccaaaatt tttcaataca aaaatatcat
    19141 tttcaataat agacaatgta tttccagttt tcatattaga gtaatatctt ccagatagtt
    19201 ttgatacatg atttaataat ttaattttaa gacttattgt gaccttattt ttcattaatg
    19261 aatataaata tgataatata agattaaata gagaagatag aacttttatt aatccataca
    19321 agattattaa ttttattaat agtgttatat tttcattatt tattccctca tctataatta
    19381 atcttgttat ttgaggcaaa aataaatcaa ttatagatat tatacacaca aatataaacg
    19441 aaaaaatcag atttactcta tttctatgta ttactaattt agaaaatcta attaaatttt
    19501 tattttccat taaaatttgc tcctctaata ttttaattta agtataacaa tattacagtc
    19561 aataactttt ctatatagtt taaat
//
LOCUS       DAEMHZ010000065        19297 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000065 DAEMHZ010000000
VERSION     DAEMHZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.065"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(250..786)
                     /locus_tag="KPK72_002202"
     CDS             complement(250..786)
                     /locus_tag="KPK72_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG2489414.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(804..2117)
                     /locus_tag="KPK72_002203"
     CDS             complement(804..2117)
                     /locus_tag="KPK72_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG2489415.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2472..2912
                     /locus_tag="KPK72_002204"
     CDS             2472..2912
                     /locus_tag="KPK72_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2489416.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3097..3285)
                     /locus_tag="KPK72_002205"
     CDS             complement(3097..3285)
                     /locus_tag="KPK72_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489417.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4320..4715
                     /locus_tag="KPK72_002206"
     CDS             4320..4715
                     /locus_tag="KPK72_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489418.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            4758..6857
                     /locus_tag="KPK72_002207"
     CDS             4758..6857
                     /locus_tag="KPK72_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG2489419.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(7082..12292)
                     /locus_tag="KPK72_002208"
     CDS             complement(7082..12292)
                     /locus_tag="KPK72_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG2489420.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLRMKEVLTELSTKFASLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGSQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNQNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12307..14802)
                     /locus_tag="KPK72_002209"
     CDS             complement(12307..14802)
                     /locus_tag="KPK72_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2489421.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLERTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTPTIER
                     FINVLAKQSGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYREEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGG
                     NAGNKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDD
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15479..15766)
                     /locus_tag="KPK72_002210"
     CDS             complement(15479..15766)
                     /locus_tag="KPK72_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489422.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15786..16061)
                     /locus_tag="KPK72_002211"
     CDS             complement(15786..16061)
                     /locus_tag="KPK72_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489423.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16698..16877)
                     /locus_tag="KPK72_002212"
     CDS             complement(16698..16877)
                     /locus_tag="KPK72_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489424.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17161..18636)
                     /locus_tag="KPK72_002213"
     CDS             complement(17161..18636)
                     /locus_tag="KPK72_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2489425.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         6433 a         3378 c         2091 g         7395 t
ORIGIN      
        1 gtgattattt tttattaaaa acgatataaa tatacaattt aatactattt tttaaataaa
       61 cttatattaa aatacattta gttatataat tttatttatt ctaagaatat aattataaaa
      121 ataaaaaaag aataggataa ttttcctact cttttttctg tacttacata taatattaaa
      181 actctttatt ttttaatata tattttaaat tttctttttt aataaatctt tttcttttaa
      241 taaatctatt taattttctt cttctatatc agagccaaag ggaatccatg cattaccaaa
      301 ctctttatct ttaaataaat aagctaatcc agttatttgc ttcaatctaa gaatttcatc
      361 aacttccata cccaggtgct ttgatatcca ttcatcagat tttccaattt cacaaagttc
      421 agttataatt ttactcatta aatccacatt atgagaacct cttgcacgat tgtgtcgaat
      481 agtacttgcc attctttcat caagagattt atcaattact gaaacaggaa gaagtccatt
      541 ttctctatca tatatatcct cattttcaag cattactcta tatctatgaa aaccatctac
      601 aataatatac ttatcctttt cctcattata ataacagaca attggcattg tatacccatc
      661 ttcttttata cttttataca acaactgcat ttctggtggg gctactgcat ttgggttata
      721 gtcgttagat tctatctttt cgattggcac tgcgataatt ccatatacag gacttttata
      781 ttccatagca tcttaactcc tatctaatga attttttcat atttcttctt aatatattcc
      841 gttctaattc tctgttcttt tgtaggacca aatcccataa atctacacat atggtcattt
      901 tttaaaatac aataacacat tcttttccaa cttggtatat ctttggtaaa tttaatatca
      961 tccgtatcat caggtatatc atccaaaaat atgactctac tctttttatc ttttgtatga
     1021 tttgaaactc catttaattc tattttataa ccttttctca acaattcacg tataactatt
     1081 tctgacaaac caccacctgt attctgccaa aactcaatag atgttgaaaa ttttttaata
     1141 tagctattcc ttatattttc aggtaaggta tcaagcaaaa aatatgtgaa acttttccat
     1201 gtgtgtccat atggcaattt aacactacga tatcccatag cttttgtttt gccataaata
     1261 gaagtaaaat taactccttg tactctagca accaatttca cccatacatc tgggtccaat
     1321 attctgtata aatttaaact ctcttttgct gaatcattaa aaggtgatgc cactctcatt
     1381 tttcttggag ctactccagc tttatagaat aagtcataaa ttttattata gtcatagtca
     1441 aatttatagt gtgctaacca tatatcttct atttcccaat catatatagg agatgccgtc
     1501 cagacatttt taaattcctt agttatccag cactttcctt tatatccatg ctgtttattc
     1561 acaatagaac aataacgttg tagagattct gctgctcgtg ttcctaataa gcaaacaact
     1621 tttgcacctt catgtatatc tctaatccat ctaccaaatt gttttgccaa actttcttgt
     1681 ctcattttat atttataata agtaaaagga ttattttttt gatttattat gtattctttg
     1741 actggcattt cccttaccca taactcttct ttctcatcat cccatggata ccaatacatt
     1801 tcataatttc ctacagcagt tcttgtagcc attggtaaac acacccaata tggttcaata
     1861 tcttcaatat tatcttcaaa tactttttca acatattcag tagtcaaact atattgagcc
     1921 tcaaaatctt gatgaaatac tcctattttg gaacttatat tattctttct cttataatcc
     1981 aaaactaaat ttagcagtaa accactatct tttcctccgc taaaagatac atagatataa
     2041 tcaaattcat taaaaagata agaaagtctt tcttgaaaag cttcaaatac atcggattct
     2101 aaatatctct tatccatttg aattatcccc tttcaattag aagtttataa aaccttacaa
     2161 tgcaatatta ttatgctttt ttagcagtaa tcagcataaa ttatacatta tttaaataca
     2221 caaatccaac taaatacata taattataat gtaattatac ttttgtatta taaactatat
     2281 tttatcatta ttttacattt ttttccaata tatataatta tcatattttt ctattagcta
     2341 aaactaatta ccttatattt ttacgtattg gtataataat acataaaaac ttgaataatg
     2401 cataaaaact tgacatactg ttgtcataat tactttgcta taattaaaag taaaaattga
     2461 ggtaaattct aatgtatgaa cgtatgcttg ataaaagcaa taaaccctgt ttgtctcaaa
     2521 taatagaata tataggtaaa gatggatatg cacgattgtg taagttggaa acttacttga
     2581 aaactcaata taatttatca agagaacttc gatttccttt tggaaacaat tatggttggg
     2641 gttacaaata tagccataaa tcttttcatt tattttatgc tttttttgaa aaaggtgcat
     2701 ttactgttct gattcagata ggtgacaagc aagtcccagc agtagaaaaa acgttggtat
     2761 ctttgacacc aaaagctaaa gaactctggg tcactcgtta tacttgtggc aataatggag
     2821 gttggattaa gtatcgtatt ttagaagatg cagacttact tgatattatt caatatataa
     2881 gagcaaaaaa atctccacct aaacaaaatt aacttttatt ttttgtgatt taaatatagt
     2941 gtatataaat tccattcttt ccatataaaa aaaataatat aaaaaatatc tattagtata
     3001 actcatttag tatgtcttat tctacttttt acagataatt aaaaagtttt atcataaata
     3061 aatcataact aggcatccta gtaaatcaaa attaatttat tcttttttat tagaattttt
     3121 gataacagtc cctatacata tgccaattat cattccaaaa attagaccat aacacattgt
     3181 aatacctaca tttcctttag aaatacctat agcacaacct atagccattc caagtatcat
     3241 gcctataaac atacatataa taactaaatt gttgttttta ttcatagttt ttctccattc
     3301 aatatatttt aaattgccta gctcgccttc ttagcttaaa acaaagaaat ataaattaac
     3361 tttctttcat tctaatatct gcctgtataa atgtcaaaca acacatttct tgtcataacc
     3421 tcctcttttt gaagtaagta caatctccca gacgcaataa tatcaatttc ctaaaaccat
     3481 ttaagtaact tttatattta cataatattt acaatcataa actatattgt tattcattta
     3541 tacatcataa acactccctc ctaatcatta ttatacttaa aataatgttc atttataata
     3601 atatatctaa cgcactttaa ttgaaaaaac taaaactttg cctttcattc ccatatataa
     3661 ataagtacac tttttgatgc tctattacaa atcaactaaa ttaattgata taaattatca
     3721 ttttctgttg aaaataccat tcgcacatgc tatacttaat tgcataaatg gtaataaatg
     3781 tcatatgatt ttacaaatct attaataaca attattactt ttactgtatt ttaaagtttt
     3841 tggactaaat atcaaaaact ttctaaaata caaagtacaa aaaatttata taagttataa
     3901 atttatatga actagataag ctcaaacaat atgttatgta taaactgtgt agtattcggt
     3961 tataaattta tataaattat ataagctata taaagtatta cctcaactat ataagctaga
     4021 tatataaatt gcatatataa gctatataag aattatttca actatataaa ctacatatat
     4081 aaattacata cataagctag atatataaac tagatatata aactgcatat ataagctata
     4141 taagaattat ttcaactata taaactacac atgtaaaatt catgtaaatt tactaatata
     4201 aatcaaataa aattactaaa ctctctaaac taattttgta aaacatactc tatttttaac
     4261 atttgttacc ttatacataa gtataaacaa aatctaacta aatgaaagga taatgtaaaa
     4321 tgaatatttt aaaaatgaaa aaaacaggaa tatttttagg aggagtactt tttggtactg
     4381 taggtgttaa gatactttca agtgactgtg taaaaaaaac ttgcactcgt gcaacagcag
     4441 gagcattgaa agttaaagat tctgttcttg aaacaactac gaaaattcaa gaaaatgtag
     4501 aagatatttt agctgaagca aaagaaatca acgagcaaga agacttatct acatctaagg
     4561 taaacgttgt acaagatact cttaaatgcg aacaagaagt tcttaaaact gaaactataa
     4621 aaactgtaga agatactaat tctaataaca ttaaatgtga agaaatcaaa gataacgtaa
     4681 ttgaagaaaa aaataatact tgtacaacag aataaattta atgattatta tgattaataa
     4741 tatcaggagg aagtaaattg aatttttata taaaacacga aataagaggt cgtatacgct
     4801 ttgacttaca acttggcaaa ttaaacaaca agcagtccga tactctctta tattatttaa
     4861 cttctataaa tggtattacc aaagcaaaag tttacgatag aactggtgat gcagtcattt
     4921 attacactgg tgaaagagaa tacataataa gagaaatcac taaattcagt tttaacaata
     4981 gcaaaataga aaacctagtt cctgaacata ccagcaggga attaaacaat tattatagag
     5041 acaaattagc aaataaactg ctagtcaaaa gtatctccaa aatatttttt ccaaagtcct
     5101 taagattttt tttaattgga cttcattcta taaaatattt taaaaaaggt ctctcttccc
     5161 tactaaataa aaaaatagaa atttctgttt tagatgccac tgctataggt atttctattt
     5221 ttagaaaaga tatcaatacg gctggttcag ttatgtttct tcttggtatc ggtgagttat
     5281 tagaagaatg gactagaaaa aaatctatag acgacttggc tcaaagtatg tcactaaaca
     5341 ttgaaaaagt atggttaaaa aaagatgcca ctgaaatact agttccaatt tcagagataa
     5401 aggaaggaga tttagtcagt gtaactatgg gaaatatgat tccattggat ggagttatag
     5461 tatcaggaga agcaatggtc aatcaggcat cacttacagg tgaatccctt gcagttaaca
     5521 aaaaagaagg ctcttacata tatgctggaa ctggaataga gcaaggcaat attgttatgt
     5581 gcgtcaagga aaaagctggt actactagat tccagaaaat agtaactatg attgaagaat
     5641 ctgaaaaact aaaatcttct gtagagagta aatttgaaca tctagcagat acattagttc
     5701 catacagctt tttaggaagt atattaacct atgcaattac tagaaaccct ataaaatcac
     5761 tctctatatt gatggtagat ttctcttgtg cattaaaact ctctatccct atttcagtac
     5821 tatcagccat gagagaatgt aacaatcaca gcattgctgt aaaaggtgga aaattccttg
     5881 aaggtgttgc atgtgcagat actattgtat ttgataagac aggcacatta acaaaagctc
     5941 aaccaactgt ttcagatata attacattcc aaaattataa taagaaggat atgttaagac
     6001 ttgcagcttg cttagaagaa cacttcccac actccatagc aaatgcagta gtttatgaag
     6061 ctgaaaaaca agggcttagt cacaaagaaa tgcatacaga ggtagaatat attattgctc
     6121 atggcatttc aacaaaggtc gatgataaaa aggttgtaat aggtagcaaa cattttgtat
     6181 ttgaagatga gaagtgtact atcccagacg gtgaggagga taagtacaac aatctatcag
     6241 atgagtattc tcacctattt atggcaatat cgggtaaatt atcagcagtt atttgcataa
     6301 atgacccttt acgaaaagaa gcaaaatatg taatttcaaa tttaagagaa tatggaataa
     6361 aaaagattgt gatgatgact ggagatagcg aaaaaacagc taaatctata gcaagtaaag
     6421 ttggtgttga tgaatattat tcagaagtct taccagaaga caaagccaat tttgttaaaa
     6481 gtgaaaaact aaaaggtcgc aaagtaatta tgattggtga tggaatcaat gattctcctg
     6541 ctatttcaga atcagatgtc ggaattgcaa tgagtgaagg tgctgaaata gcaagagaga
     6601 tttcagatat taccatatca gcagataatt tgaataattt aatcgtttta aagcagatta
     6661 gtaataagct tatgaaaaga gttgatttaa gttctaagtt tattatcggc tttaatttag
     6721 gtctgatttt acttggcgta agtggatttg ttagaccaag tacatctgcc tttttacaca
     6781 acgcatctac tgttggaata agtttaaaca gtatgacaaa tttattggaa aacaccaata
     6841 cttataacta ctattaatcc ccattatata aaacatatgt atataaaaaa tttataattc
     6901 atcttttaaa aagaaactgt cttaaaatta gcttttttct aatcaaaatt atataaaaca
     6961 tattttacaa attttaaact caacttattt ctatttttta caaaaaggag atgattcaaa
     7021 ttttcatttt gaaacatccc cttttgtaaa tttctttaaa acttatatat taattatatt
     7081 ttcaaccaat aattgtattt tcaaccaatc tttcttcata gcattttaaa tcattttgct
     7141 ttagataatt aaattcagac tctacataaa taccacagcc taccaattca attgtcaaat
     7201 taggtatatt ttcctttagt atactagtct gctcataaat aattctttcc aataaatcaa
     7261 cttgtaatat agaaaaatgc ttcataatct ccccatcact atctatatct ttcttgattt
     7321 cataccaact ctttgacaaa attcccttca aatcaatata ccctagactc ttcaatatat
     7381 ctatactctc gctccatgca tacttactag gtttatcaaa gtatgattct agtttcttct
     7441 tctgctcatt tgtaagtgaa tcataagaaa gcttcttatt aaaatgtgcc ttcaaagaaa
     7501 tcttagccat agaatatgga cttctaccag agtttaatcc tgcatttttg aacttttcaa
     7561 ttcctttatc ttctactgaa gaccatattg agtttattga atctgaatca ttgtatttaa
     7621 catcaagacc atgtgtaata tctatatagt tttgtggaaa tacatattga taatagacat
     7681 cttttttatc atcatatagg gagaagtatc cactatcata taaatgaata tgcaccttta
     7741 cttttaaact atcctctaaa atatttcgtc taaggttcgg gatatttgca tacatggtta
     7801 catattttat ggcatcaaag ttttgattta atggaacttc aaaactaatt tcatctcctg
     7861 tcaaaagctt tttcacttct tcttcattac aatcatatag agaatatgta ttttcaaatc
     7921 ccacacgtga tttcccatct aaacttcctg ttaatgctac cttcaaaata tggttatccg
     7981 ttttttgcca ttggtaagca tcttggtcaa gaaactctct aatctgtcta ttttgctctt
     8041 tcgcctgatt tataacctcg ttttcatctt ctgtaatccc tacatggcat ctatttaata
     8101 tatttactat attgcttcca aatgggaatg tccatagttg aaaagctgct gcctcaacct
     8161 gacacaaata tctaacctga tttaattcaa tttgatggat aagttttttc tgatattcat
     8221 tcatctgact accactacct gacatttgat aaatcaattc tcccattgca agattatctt
     8281 ttttcttatc aatcttatca gtaatgataa tctgagtttc catcatatcc tgtacttttt
     8341 taaatattat agaaatctcc tctgaatttt ccatttttaa gcttgcaaac ttcgtactta
     8401 attcagttaa aacttctttc atcctcaatt tacttatctc attttcaaaa atatcttgat
     8461 ttttaactgt actatcaaat ttttctacaa atggtataac agactctttc aaagttttat
     8521 ctttaaactc caccatttga gtattcaatt catctagttt cttaatatta ttttctttat
     8581 ccttaattgc ctctttaact acctctattt tactctcaaa actagatttt acattaagac
     8641 aattattaac accactaatc gcagtattta taagagaaca agcagaactt ccaatactca
     8701 atgcattttt aattttagat gtcttttgct gtctttcaat agcttgttta cttttctcta
     8761 gtgcagctaa cttatctttt tgctgtttat tattgtaatt tgcctccaac atacctattt
     8821 cttttttctg gatttttgtg taaatttcct tatactcgct ttcacttaac ccctcttttg
     8881 ctctttgaat atcgtcagct ttttctgcat agttatcatc atctatagac ttaactataa
     8941 tctccttaat ttggtcaaat ttagttcttc tttcttcatc aacacctgcc aaattataag
     9001 tcattttatc taaagcattt tttctatctt cacttagctg ttctccaagt acaaatctca
     9061 taccatcaga atccaattta aaatctgaaa aatcaaccat cctgttagtc cattggtatt
     9121 cctcttcttt agctgaaaga ttttccttca aagattttgc actgtcaaat gcccctagac
     9181 aagcttgacc aacaccacta aatgcagtca gtaaactcaa tgcgctttgt attccagaaa
     9241 gaattaactg tgttttcatt tgttcctcaa gttttgcctg tttctttttt agttcctcta
     9301 tgtcttcttg ctcatcttct ttcatcttta taatttcttg aatcaacagg ttcatctttg
     9361 cattaacaga aagcatttta ttctcaagtt catcttttaa aacttctatt tgttgatttc
     9421 cagattcaat ttgagttagg atactttcct tataagtatt gaaatatcct tcttttaact
     9481 catctttctt tccttgttcc aattctctta aggtcttgtc cagataatct gtcaaagaac
     9541 gaatatctgt aacacgattt cctccaattt cactttcttc aatcatctgc acatagaatg
     9601 ctgttttttg ttcaagtgca aataaaatca tactccattc gctaaaattc ttcttttctc
     9661 ctataaaaga ttctattcta tcagactgca tcaatttatt tataaggtat tcatagattt
     9721 cctttgcctc cttataaaat tctttcttta tattcatatc atccagagaa agcactgctt
     9781 tatttttctt ctcaaaacta tagtacttct gaagtatttc tctatttaat tgcatctcta
     9841 ttttctcaac tgcatgaaaa atttcataaa tatgtgaaag ttctaaaata tcagcttcta
     9901 aaattcctac tgcacacaac agtctaagat ttctataacc tttaattcca gaaaaaccaa
     9961 atctctcgaa aaattcatag aagaaggtat ctattgacaa agaatactcc tgctttaaca
    10021 attccttttc ttttaatgca tctccagagc gaccgtcttt tccctctgct ccgactaaac
    10081 ctgaatcttc acgtttgcta attgtccagt ccttatgttt ttcataaaga gcatttaatt
    10141 tctctccatc atccgctact tggttctcat ttgaagcatg attaaacaca tatctatcaa
    10201 aataatagta atttctatca ctgtcatcta ttggagcaga ggtacctata tatcttattg
    10261 tttcttcata tatacctcta attccaccaa gacctcctgc accaccagct cctccagagc
    10321 ctccttttcc tgctagacca tgatttccat cttttttact tatagcacct tttaataaag
    10381 aagcttttcc aaggagataa acctgtccag acttgctccc aagacctgca tttccaccat
    10441 tacctcctgt gcctccagtt ccaccttgtt ttcctttttc tgcttttacc acatagtatt
    10501 ttgtaccttt aacattagca aattcttttg actctttttt agaacctatt ttagcatttt
    10561 ccccagcttc tccagttatc cctgtatctc ctctttttcc atcttgtcca tttccaccac
    10621 gtccaccatc tacgtaaagt ttaagttctc ctcttttttc tatttcttta aaagataata
    10681 aaatatcacc tgaagtacct gcattattgc catttcttcc attcattccg tcgtaaatag
    10741 aaccttctcc aaccaatttt tctgcatcct taccagatac gttgattgaa taaatccctt
    10801 ctggtataca taatttttct gtaagtagta caatattctt acctgagaag acttctttct
    10861 ccatattaca gtcaattttc aactcacaat ctccatacat atatatagat gacaaaatat
    10921 taagttgata accagatatt attttagtca aatccgataa attcacttta ggtataggaa
    10981 tatatataga aagtacattt tctttctcat atgattttat atatgtacaa ctttgaatct
    11041 tctctatcct atactctgca tcttcaagct ttttggcttc ttcttcccta tattcttctt
    11101 ctgctatcca atatttgata tcttttaatg tatcttctaa agtatttttt agcaatgatt
    11161 ttaacaactc acttagacca acaatatgag tctcactaca gtagctattt aactctaaaa
    11221 gaagacgtgt ttcttctaat atattcactt tctgtacgcc atcaccatat gaaagttctt
    11281 gtacaaactc cataagctct ttcatattat tagtcctact acataaatct gtatacatct
    11341 tatctaattg agtcttttta tcctttagac tatttataga agatagaatg gaaatagaat
    11401 cttccatacc ttttttgaca ctttctatat aaggaactaa aagtttttct aggtctttat
    11461 aggttacatc aacggctttt tgtatttcta ttttacaaga agcagtaagc atcaatccca
    11521 aatctgtttt ttcaaatgtt tcaggtagat ttttcatttc atccaacaac ttttctggct
    11581 gtttatctag tggattacat aatttaatag atacaccatc tgataatgct acatttaata
    11641 agtacactaa ttccttacaa ccgtcgtaat ctttctcttg actatccagg tcttcttttg
    11701 ctttttccaa tatatttctt attttttctt ctgttacatt accgattagt gttttaggta
    11761 ttttattgat aagaaagaag aaattttctt tatactttgc ataatcacca agcatatctt
    11821 tcaaattttt gcctgtttct atcaataatg tatgtctaga gtccagtaag gattgatagt
    11881 ctaaaataac taccatccct ttcacctttg aagatgtaga aatagtttcc cttacaagca
    11941 tagcaccagc aatcatatac tctgctccac ggttatcaaa aaaacctggg caatcacata
    12001 atgaaaaatt tctatttgca atatctacaa cttctggaca taaagtctta gaatatacac
    12061 catttccaat ttcgacagct gaatcttttg cagttatata tatttgtcca gtctcttcat
    12121 ccgttttttc ttccatcacg caacctttta aataatttat acaggtactc tttccaacgc
    12181 ctgaagcacc taagaataac aaaattgact tttcttttaa gtcatatttt ctaagctcgt
    12241 ctcttccttc ttccaaaagg tttacgattt cactataaag tttatttgcc atacatccat
    12301 ccctttctat atttcggaac aatagccttg atatttttct ttaataagat tctcttctac
    12361 ctttgaaaca gctgttgata acttagtatt tggagcatta aatcctccgt cgtctcctct
    12421 atctccatat tcagcgtatt ctcttgctgg tcctgttttg ccttcattcc aacttccgcc
    12481 actaaattct atatgatatt taattatact atctaactta taatcccaac ttgtaaaata
    12541 tcctgtatag tacacagtct tatctatacc acttggagaa aatgaaagtg tattatatcc
    12601 gtgatagcca ccacttcctc catatccacc tgaacctggt ctaccatttg gacctgcttc
    12661 tccatctttc actgttatat cacaatgatt aattattcta tttttttgtt cctctgagtc
    12721 tgaaatgaag taaatattac ctttattacc agcatttcca cctcttccgg catttccacc
    12781 agctccacct ttgtcacctt ttttaccact tgtacctttt gcaaaatacc ctttaaacat
    12841 ctgtccatgg aaactacttg tatatcctgc atcagctcca ttacttccag aatctcctgt
    12901 ttcaccattt ttcccatcta ttcctttaga accatttcct ccatttgcaa tcagtgttaa
    12961 acgataattt ctaatttctt ctttaatata taagtaaata ttacctgcat ctctaccatc
    13021 ttcaccattt ttcccataca tagaactatc tacaagttct ccgctctttc cacttacatt
    13081 aatttttaca ttactaccta catcaagatt gttacaagca agaataatat ttttctctga
    13141 tgcaaagtcc attccaaggt tatcatccat ataaatagta tctttggcaa agaacataaa
    13201 tgtagaaaca ttgtcatcta cttttctatt tttgaaatat tccaatacac tactaattac
    13261 taagtttgga attaatgctc ttatttcaat acctttctta tcattaatat taattgtttt
    13321 tactataaat ggatttaaaa tgtatttacc aataaattct ctaattatct cattaccttc
    13381 ttctctatat gcacttaatc ttgttatact gtcttctaac tcttcatttt gtgtaactgt
    13441 aacatttgtc aaaattgaat taaaatcact tacactatat acataatcct ttttatttgc
    13501 aacttccatt aatttttctc tatttggtaa tgtaaatacc tgtttttgta cttcataaga
    13561 aactaaaact ttttctaaat tctttcttat tataatgaag ttttgttcca ttatcttctt
    13621 acttgccttt tctataataa attccatatg tacagaaggt aataaaattt ccccagttcc
    13681 atgttctgta tatttactta aagcatcaat tatatctaag tttcctttta tcttattttg
    13741 taattccatg caactatctt gtaagaataa gccactttgt ttggctaata cgttgataaa
    13801 tctctctata gtaggagttg ttgtactttc caaccagttt ttaaatactt ttacaacctc
    13861 atcatatttt gctatttctt gaatcattgt tatacttcca ataatactga taaaatcttc
    13921 tgtatacgcc ttaacataat cttctaggct agatacgata ttgttttgaa tttcttttat
    13981 caacgtatct aaaactaatc ttgcatctgc actaattgga tattggaatg ctgatgtctt
    14041 atctttaact tcatctaatt ctagtacttg ctttaataag ccttccctct catctgatgc
    14101 aagtggctta caaagatgaa tattttttcc attatctttt agcaattctt caaataacca
    14161 agctttttcc tctatattac tgtctaaatt ttcgcataat tgttcatatt ttctttttat
    14221 aacattatat atttgttttt ctgttctttc taagaaactt tttgaaatga aaaaagttat
    14281 actaccctta aaattttctt tatgaagaca catatttaac tgttggaaca ggtctagtac
    14341 ttttgtaagt ctagagtcta atatatctgc ttctggtatc aatacaatta ctgcacatat
    14401 agatgcagaa tttttaaata cttcacctaa tgaaactgct gcactaatat catgggctac
    14461 tcccctattg tcgctaaaac ctgcagtatc acataatttt gcatccattc cttctattgg
    14521 ttttctcata tttacaatgt tcgggtataa agtttgagaa acagctcctg aaccaatctc
    14581 tgccacaggg tctaagcaat caatatatga tataccagta tcttcatctt ctacttccat
    14641 cattacacat cctgcaagat agttaattgc agtacttttg ccacatcctg tcccaccaag
    14701 aattagaatt aaattttgat tttcagcctt atttattgct acttttcctt catttaataa
    14761 gagtgttatt tcttctaaaa gttgattgtt attttctggc atttattttc ccccttggtt
    14821 atatatttta tttaaataaa gtttaatccc ttaagtagat tgcaaagttt ttagactata
    14881 tttaaatttg ttttttattt ttaataaatt tgctttttca ctaaatttgt attttcactc
    14941 aataattatt cttctttgtt tattaaaata actgtgttgt ataatataat ctttaccaac
    15001 acagttattc tatttaatgc gcttatttta ttgtatttta tgtaatacag ttgtttttta
    15061 tattatatgt ccattatttt cctgtggtta ttacatatag atttttaatt atatttgtac
    15121 cattaattta aacattccta tactactgct taagttttac acctatacca gcttgaatat
    15181 tttattttta tttgcttaaa tagttcaact atactggttt aaataggtta tctttatttg
    15241 cttaaatagt tcaactatac tggtttaaat aggttatctt tatttgctta aattagttca
    15301 actatactgg tttaaatagg ttatctttat ttgcttaaat tagttcaact atactggttt
    15361 aaataggtta tctttatttg cttaaataag ttcaactata ttggtttaaa taggtttcct
    15421 ttagtttaaa taattcacct atacctactt gaatagttca cttttatttg cttaaattct
    15481 ataccaatac cggcttaaac attatactac catgattgta ttgtctatct tcaactacaa
    15541 taaaaacagg ttctaaatct ttagtagatg ttatttttaa ttctaaaaaa atattatcag
    15601 ttggtaatat ttttacatca taattattta aattattatt cgtgatacta ttaaatacat
    15661 caccttgacg ttcaaaaaaa acattatcaa ctattacaaa ataaacatct tcttttctac
    15721 tattttcaac ctctatagta ttttgtttag ttacatttag tctcatcttc cacctcctat
    15781 taatattact taattatatt aatacttgta attgttgcat tatgttcatc acaaggaaga
    15841 ggtttaaaaa caactatctc tgctaaataa ttctgtggag gatttgtaac tgaagtagca
    15901 ttgcaaataa atttaaaatt tgttcctgct acaacttgag tagaaacaat aagtggttca
    15961 taaccaaccc cttctaacat tcctacagct tcattaaata cttttaaatc ttcttcttcg
    16021 acagctctga attttgacca tccaccaaca aaaagactca tataaaaacc tcctttatat
    16081 atatatttat ttatatataa agtagtacca attttctcaa tattcaatat tttctgtgta
    16141 actggtcaat gaaaatgcat aactggataa aaatacttat aaaataactt aactttacat
    16201 ataaatagct tactttacat gtaataagat tattctacaa tatgaaatgc atcttgtttt
    16261 gaatatattc taaatacaat aatggatatt ttcaattatt ataattagaa aatatccatt
    16321 acttaacttg ttttatttaa aatcacatag agactttatt atttttttta ttgcatgaat
    16381 agctatttca ggtatcataa ggtgatatac cccctccttg aaaccacaaa aaacatgaca
    16441 aactttacga atcttgtctt aaaaaaccac aggtacattc cttatttgtg gattggagta
    16501 gccatcacaa gcaaatttta caatttttat gatgacaaaa atcaataaaa tcgcttagta
    16561 tataacacga aatactactt gttactatta agaatagtta gataggaagt atgcattata
    16621 caaataaagg aatcaataaa gaaagttaaa acatccctac aaatcaaaaa tctgttacat
    16681 ttttctatat ttaaacgtta tttacttaca attggagaaa taacaggttt tccttctcgg
    16741 tcaagtaaag gcgtaaatcc ggctgcatgg ccataatagt ggaatacata gttcacacct
    16801 gtttctttat ccacccaaat ctccgttgca tttatttttc cttgtgaata tactttttca
    16861 aatctatctt ttcccatttt ataatcctcc atattttgat tggtctatac cttcttcact
    16921 taagtagaaa aaatacataa cttatttttt taattctaat atgagcacgt aaaaatgtca
    16981 aacagcttat ttaatttcat aacttcatgt tactgttcgt aaccttgtct gttcacttac
    17041 actacattac agcaccattt tactctttac atgattgtat tgcacacagc aaaaatgctt
    17101 actttattgg atttttcaac taaaaaaagg attttttctc cacagaacat ttactatttt
    17161 ttataatata tctaatttta ctctcatatc aacatttaat gaagatataa cctttttcaa
    17221 atattgtaaa gactccttaa aaatattaga aaaagattct gcaaatctct caacatctcc
    17281 tttttttaaa tacctaagta tctcaaaaca tacatcaaaa cgtgttttca aaatttcatt
    17341 atattcttca tgaaaaaatc tctctaaata gattccaaat atcatatatt gttttaaatg
    17401 caaaaatgtt tcctgtaata cataaatttg tgtataatct actatcttac aaagtaaaac
    17461 aaaatctgga taaaccttac actcttcaca attattttgt aagtacctta aatgagtttc
    17521 tattttatct acagctatca aatccatttt atttcctagt aaaagtacta catcctcaag
    17581 agtaatcgta acaaactcca tagcatccaa aagcaaactc aaccttttag ctccttcttc
    17641 tgtatttata taatcatcat catattcaga aaaaagcgtt acatatgttc ccttgccatt
    17701 aattgtttta gcaattccca attcatttag ctcattatag gcacttctaa ctgttggaag
    17761 tgatacatta tatctattaa ttatcgaagt aattgatggt agacaatctc ctattttaat
    17821 attgccttca aaaatttgac aaattacatc tgaaacaatt aagtcatata aataacattt
    17881 ttccaacgtt acagaattaa aactacttgt atctatactt tgtggagcac gattttctac
    17941 cattttaagt atggattctc tacaagcttt atacatatat gttaatagtt ttttaaattc
    18001 aattagattc ccgtccaatc catataagta agtttttctt acctgctcta tgtatttatt
    18061 acgaaaaaag tctatacaac aattatccaa gttatccaag ttatcgaaat tataggccaa
    18121 ttgtgaaata atgacatcat gttgaataat ctcacaaaaa tggtgaatca ggaaattatt
    18181 tgttttactt attagtttat ttatatattt tagcctttgt tctttaaaga ctaaataatt
    18241 agaactattt ttttccatac aatcgataat ctcgtttaat tcttcaaagt ctttagatgt
    18301 aaatctttta atattgtaca tagcaataga aggaaatatt aaactaaaaa tcttatatat
    18361 ttctgatatt tgtaaaatct tctcatataa taagtcagat tctattgatt gtttactcgt
    18421 actctggatt aaaactggtc tagaaccagc ttttgtctct ataaagccct tttctgagag
    18481 catttttatt acagttttta tagtattttt tgataccata tattcttctg acatgctttt
    18541 tatagaatca agcctatctc caatagtaat tttaccagta taaatttgtt gaattatatg
    18601 ctcaaaaata attaaatatt gtggttttct cttcatattt ttcctcttcg ataaattaga
    18661 tttattgttt tttgtaaggt atatttactt ttatttaata gggcaaaata gttgcttatt
    18721 ttttcctcat aatctataat taatataaac catttatata tatggtctaa gttttataat
    18781 actgtaatgc agtaaaatag catattgtag ctatactaca gcaaattaac ataccattgt
    18841 agtatattaa ttggaataat tttaagtact tcaaaattcc agcaaaacat atatgtgttt
    18901 ttgatattat gtatattcgt taatagaata gctatacttg ttttacataa taaattatat
    18961 gatacttgct atatgtaatt actatattta atacacataa ctattatact tactatatgt
    19021 aattactata tttgatacac ataattatta tacttactat atataattac tatacttgga
    19081 cacataacta ttatacttgc tatatgtaat tactatactt tatacacata gttatcatac
    19141 atgctatata taattactac acttggacac ataactacta tatttactat atgtaattat
    19201 actatacttg gacatatagt tatcatacat actatatgta attactatac ttggacacat
    19261 aattattata tttgctatat gtaattatac tatactt
//
LOCUS       DAEMHZ010000066        19286 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000066 DAEMHZ010000000
VERSION     DAEMHZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.066"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            202..780
                     /locus_tag="KPK72_002214"
     CDS             202..780
                     /locus_tag="KPK72_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2489426.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            819..1397
                     /locus_tag="KPK72_002215"
     CDS             819..1397
                     /locus_tag="KPK72_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2489427.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1483..2112
                     /locus_tag="KPK72_002216"
     CDS             1483..2112
                     /locus_tag="KPK72_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2489428.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2102..4861
                     /locus_tag="KPK72_002217"
     CDS             2102..4861
                     /locus_tag="KPK72_002217"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2489429.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4948..6510
                     /locus_tag="KPK72_002218"
     CDS             4948..6510
                     /locus_tag="KPK72_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG2489430.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMKDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6511..7647
                     /locus_tag="KPK72_002219"
     CDS             6511..7647
                     /locus_tag="KPK72_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2489431.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7711..8787
                     /locus_tag="KPK72_002220"
     CDS             7711..8787
                     /locus_tag="KPK72_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG2489432.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFADALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8765..10003
                     /locus_tag="KPK72_002221"
     CDS             8765..10003
                     /locus_tag="KPK72_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG2489433.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10057..11145
                     /locus_tag="KPK72_002222"
     CDS             10057..11145
                     /locus_tag="KPK72_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG2489434.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11173..11964
                     /locus_tag="KPK72_002223"
     CDS             11173..11964
                     /locus_tag="KPK72_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG2489435.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12079..12633)
                     /locus_tag="KPK72_002224"
     CDS             complement(12079..12633)
                     /locus_tag="KPK72_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2489436.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12828..14030
                     /locus_tag="KPK72_002225"
     CDS             12828..14030
                     /locus_tag="KPK72_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG2489437.1"
                     /translation="MKSFVSPDNIWVLWAIVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14086..15066
                     /locus_tag="KPK72_002226"
     CDS             14086..15066
                     /locus_tag="KPK72_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG2489438.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNIGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15314..16267
                     /locus_tag="KPK72_002227"
     CDS             15314..16267
                     /locus_tag="KPK72_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489439.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALFTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16257..17213
                     /locus_tag="KPK72_002228"
     CDS             16257..17213
                     /locus_tag="KPK72_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2489440.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17206..17964
                     /locus_tag="KPK72_002229"
     CDS             17206..17964
                     /locus_tag="KPK72_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2489441.1"
                     /translation="MDDRDKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTV
                     LSIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRF
                     PYSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILL
                     DEPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKV
                     GRTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18056..19021
                     /locus_tag="KPK72_002230"
     CDS             18056..19021
                     /locus_tag="KPK72_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2489442.1"
                     /translation="MNKKAAIVAAVAIIGLVAVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7656 a         1951 c         3427 g         6252 t
ORIGIN      
        1 aaacatagct tttatacatt taataaagta tgtaaaatct atgtaaaaag taagaaatat
       61 aaacattttt ttaattatat tttaaaaata tcaagcaagt tttattcaca aatgaataaa
      121 aaatgttata atatgattaa agaaaaagat aaataattag caagatttgt ttagctaaaa
      181 ttaacaaggg agcgataaat tatgggtata acattatcaa aaggccaaaa ggttgattta
      241 actaaaggaa atccaggtct taaaaatatt ttagtaggtt taggatggga tactaacaag
      301 tatgatggtg gatttgactt tgatttagat acagcagcat ttttaacagg agcaagtgga
      361 agtgttacta atgatggaga ttttgtattt tacaacaatt taaaacatac atctggagca
      421 gttgagcatc taggagataa cagaactggc gaaggtgatg gagatgatga acaaatagtt
      481 gttgatttat ctaagattcc tggagaaata agcaagattt cttttactgt tacaattcat
      541 gatgcaacag aaagaagaca aaattttgga caagtaagta attcttatat aagaatagta
      601 gataaagaca caaatgaaga gttaataaag tatgaattag gagaagattt tagtatagaa
      661 acagctatag tagtagctga aatatataag cacaatggag aatggaagtt taatgcacta
      721 ggttctggct ttgaaggtgg tcttgctgca ttatgtggaa actttggaat aaacttataa
      781 tttcaactat aacaataaac taaaggggga tattttccat ggctatagta ttaaaaaaag
      841 gacaaaaaat cgatttaact aagggaaatc caggtcttaa aaatataaaa cttggactag
      901 gatgggatac aaattcattt gatagtggct atgattatga tttagatgta agtgttttca
      961 tggttggaga atctcaaaga gttgaaaaag atgaagattt tatattctac aataacttaa
     1021 aacatccatc tggagcagtt gaacacttag gtgataatag aactggtgaa ggtgatggag
     1081 atgatgagga aatattagtg aatttagaga caatgccaaa gcatatacaa aagatagctg
     1141 taacagttac aatatatgag gctgctgaaa gaagacaaaa ttttggacaa gtaagcaact
     1201 catatataag agtattaaat tctgaaaatg atgaagaaat attaagatat gacttaggtg
     1261 aagaatttag tatagaaact gcaattacag tatgtgagat atataagtat aatggagaat
     1321 ggaagtttag tgcagttgga gcaggatttg aaggtggctt agaagcactt tgtagaaact
     1381 atggcttaaa tgtttaattt aagcaatata atatattaaa tataaataac ctatacaaat
     1441 aatgtatagg ttatattata taaaattttg tggaggttta gtatgtcgat taatttatca
     1501 aaaggtgata aaatagattt aaaaaaatct aacccaggac tgagtaacat acttgtaggt
     1561 ctaggatggg accctgtgca acaatctgga ggaggttttt ttaaatcgtt atttggtgga
     1621 ggacaagctg atatagattg tgatgcatct gtatttatgc ttaatcaaga aggtaaatta
     1681 agtggaataa aagatttaat ttattttgga aatctaaaaa gtgcctgtaa atcagttttg
     1741 catacaggag acaatctaac tggtgagggt acaggtgatg atgaacaaat cctagttaat
     1801 ttggataaag ttccatcaaa tattcataaa ttattgtttg tagtaaatat atacaattgt
     1861 gtagatagaa aacaacactt tggaatgata gaaaatgcct atataagagt tgaagaccaa
     1921 ggtaataaga aagagatagc aaaatacaat ctatctgata attattctga aaaaactact
     1981 cttatagtag gagctatata tagaaaagat ggaagttggc aatttaaagc aattggtgaa
     2041 ggaacaaaag atgcgggtct taaagaagta atgcaaaatt tagacaggat tgagtgtgca
     2101 tatggaattt aataatgata tcaaatatta taatgtagat aaagaagaag ttacaaaaga
     2161 cttatctgtt aatcctgaaa aaggtttatc agaatcagaa gtcaagacta gaagagaaaa
     2221 atatggtttg aatgaattta ctccaaaaga agagggtagt ttttgggatg atttaaagga
     2281 aagtttaagt gaaccaatga tagttatctt gattattgca gcagtagtaa gtgcagtaat
     2341 tggggaaact catgatgcaa tagggattgt aggtgctata gcaataggta tagcaatagg
     2401 tatgattaca gaaggtcgtt ctaagaaagc agcagaggct ctttctaagt tgactgaaaa
     2461 catagaagta aaagtattga gagatgggga agtacatcaa atttctaaga gtgaattggt
     2521 accaggtgat atagtatata ttgaaactgg tgacatggta cctgctgatg gtagacttat
     2581 agaaagtata aaccttaaaa ttagagaaga tatgttaaca ggagaatctg atgacgtatc
     2641 taaaaagtgt gatgtagtag tttctatgga agagatagaa tctaaaggag cagtagtagt
     2701 acaggaacca atacctgcaa aacagataaa tatggtgttt ggtggtacac ttgtagctta
     2761 tggtagagga gctctagttg taacttcaat tggagataat agtgaaatgg gtaaaattgc
     2821 tcaaaactta tcagaaacag atgagcaaac tccacttcag ataaaacttg gaaatttagg
     2881 tggtttgata gccaaagttt ctagtgcagt agcaggatta ctatttatat ttatggtttt
     2941 ccaaatgata agtaagaatg tattaaatgt agatatgtct ggaatattac cgtttttaga
     3001 atcaatagac ccagttaaaa cagcatttac agtatgtgtt gcgttgatag ttgcagcagt
     3061 gccagaaggt cttccaacta tgataaatat gacacttgca ataacaatgc aaaaaatggc
     3121 taaaataaat gcattggtta ccaaaaaaga agcttgtgaa acgataggtt ctgtttctgt
     3181 aatatgttca gataaaactg gtacattaac tgaaaataga atgactgtag aagttgcata
     3241 tgttgatggc agatatattg aaagttcaga agaggaaata aatagttatt ttgaagaaaa
     3301 ctgtatgata aactctacag ctgatgtaga gcacaatgat ggtgatatta agtatttagg
     3361 aagtgctact gaatgtgcat tgcttttata ttacaaaaac gtagattata gacaagttag
     3421 aaaaaattct aatatagtag ctcaaaatcc ttttacttca gattcaaaga gaatgacttc
     3481 tgttataggt caagataatc accatgtact attatcaaaa ggagcaccag aagtcttact
     3541 tgaattatgt tcacatattc aaagaggaaa agacatagtt ccattaactg aaaatataaa
     3601 acatgagatt ttagaagaaa taaagaaatt gcaaataaaa tcaatgagaa ctttagggtt
     3661 tgcatataaa gaaatctcac aagctgaaga agaagcagca gtaactgctg aaagtgatgc
     3721 tatgaatgta agtgcaatgg aaaacaattt agtatttagt ggttttgttg gtataagaga
     3781 cccacttaga aaagatgtta tagagtctgt aaatacagca aataaagctg gtgtttctgt
     3841 aaaaatgctt acaggagata atataaatac agcaagagct ataggtgaag aattaggcct
     3901 attaaaaaat aatatgagag tggttgaagc ttcatatata gatactttag atgatgaaga
     3961 attaaaacaa gagatacaaa gtatttcaat cgtggcaaga agtaaaccag atagtaaaat
     4021 gagaatagtt tcagctctgc aaaagagcgg agatgttgtt gcagtaacag gtgatggtat
     4081 aaatgatgct ccagcacttt caaaagcaga tgttggtata gcaatgggta tatctggtac
     4141 agaggtatct aaaaacgcag cagacataat tttaactgat gatagtttta gtaccattgt
     4201 aaaaggtatt aaatggggta gaggaattta tgataacttc caaagattcg ttcaattcca
     4261 attaactgta aatgtaattg catttttagt ggctataata tctcaagtta tgggacaaga
     4321 gatgccattt actacaatac aattattatg ggtaaacata attatggatg gaccaccagc
     4381 actagcatta ggtttagagc cagttaggga ccatgtgtta aatagaaaac ctgttgatag
     4441 aaaagccaat ataatttcta agtcaatggt gtatacaata gtattaaatg cattttatat
     4501 aacagcaata ctaatgttac aatctacatt taatttctta ggagttgatg aagcgcataa
     4561 gggaacagta atgtttgcgt tatttgcttt tagtgcactg tttaatgcat ttaactgtag
     4621 agagtttaat gcagatagta tcttccctaa ttttacacat aacaaattag cactacaaat
     4681 aatagtgttg acaggaattg cacaagtaat atttactcag ctattccaag atttctttaa
     4741 ctctgtagca atggatttta tgacatgggt aaaagtttta tgtgtagcag caagtgtaat
     4801 tgtggttaat gaagttgtaa aatgtataat aagactggta aaaccaaaaa agactgctta
     4861 aaacaaattt tttagaagaa aacagactga ctcaaaatat gaattttggg tcagttctga
     4921 ataaacattt aaattgggag gatagttatg ggtgactcaa aggtatttga aaaaatattc
     4981 tccagccaat cagatagagg aaattacact ccttcaaagg gatatctaag ttattttatt
     5041 tcgtatattg gtttagaaga tgaagttttg tacaatctcg agatatttaa gacaaaacaa
     5101 aatattgata gcaaaaaaga tattgcttta tttactgatg ttatagcaaa tcctagtgat
     5161 tttgatatta taaattattt taaaagtgga cttcaaaaat atagaactag catgaaagat
     5221 gtagatataa atattttggg atttgaagag attgattata aaataaagca agcaatggat
     5281 agagtgttaa aagaagagga aaaagaattt actaatgata gagttaaaca aaattttata
     5341 gtgaaaataa tggcatggat aaaaatttat atcggtgctt tagatataaa taaaaatgaa
     5401 gctccaaaag taatatttta tggagatata aaaaaacatg aagtttattt actcttaata
     5461 ttatacttgg caggttttga tgttttatat cttaatccaa attcaaagag taatatagat
     5521 attttaaaat cagaaagata caatatagag tttgaagaag ccaacataat agaagaaaaa
     5581 atttcttttg aagaaagggt tattttaggg gaaaaaatag ataaaagttc tgtaaaaaaa
     5641 gcttttactg ttggggcaga agcatcaaag cgtataagtg aggaattgtt aaatgatgca
     5701 ggatttataa aaccttggca actccaagat agaaaaataa aaaatctact tctaagttca
     5761 actgttgatg aaatatcaat atactggaat caaccactta aattaagacc aggatttaag
     5821 tttaatgatg ctattgtaga agctccaaat tttctttcta agattaatgg aatatataac
     5881 gataagaatg aatatattaa gtttttagat ttattaagag attcggaaag ttcaacattt
     5941 attgaattta atggagatgt agatagattt tcaaaagctt ttacaagaga agcttttagt
     6001 ttatcctttt tattggattc aaaaggtgca atagataaaa actctgtttt aaataataaa
     6061 gattattcca tatcaacact agcattgaat caacaaatta tgattttaga gaaagtggaa
     6121 gagttgttag aaggaagtat gtttttgaat ggtttaagtg gagaagacaa gataaaaggt
     6181 ctatttacag tacttcacat ggataagaaa tttgttcata tgatgaacaa tttcgattac
     6241 tcacttataa acccaaaatt gattatatac atgtacaaga gtattgtatt tgataaagaa
     6301 atagtttttc ttatgctttt acttagtaag attggatttg atattatcat attatgtcca
     6361 ggaggagaaa acaatataga gaatgttata aataaccaat tgattgatgt acacagattg
     6421 gataaaatgg tatatgacct taaattaaat tcgcttgaaa atgatatacc tttacttaaa
     6481 aaaatttttg gaaagaggag acgattttag atggggataa atataaatag tttttcaaaa
     6541 gataagaact ctgtagaggt tactgagaat ttgcctacag cagttcaaga agaaaacttc
     6601 gatataatgg aatatacaaa taataaaaaa aatgaactga gaaagtctaa ggaagttgag
     6661 gcattgacat ctcttataga agtagaaaat ccagatacga ttttacagtt tggtaggaaa
     6721 gcatctgaag gagtagcaag agtttctgat agccttttaa atacaataaa attaaataga
     6781 aatgaagaaa attctaaaat gctggttcat ttaactaaaa taatggacaa gtttgattta
     6841 gacgattttc aagaaactaa ggaaccaaat ttcgttcaaa aacttttcaa aaaagctaat
     6901 aatgccattg aaatgatgtt tcaaaagtat gaaacattgg gaggagaagt tgagaaaatt
     6961 caaattgagc ttgaacaata cgaaagagat atagcacttt ctaataagca aataggtgct
     7021 atgttaaatg aaaactttga gttttacaat gaacttcaaa aatatattgt tgctggagaa
     7081 atggcaatag aagaaatgga taatgaaata ctacctttct ttaaacaaaa atcagagact
     7141 agtggagacc aaatggatgt tgtcaattat caagaactat taaaagttta tgatatgtta
     7201 aatcaaagag tctatgattt aaggatagca gaaaatatag ctatccaaac aatacctatg
     7261 cttagaggta tgcaacataa taattatggt ttaattagaa aaataaatag tgcatttgtc
     7321 gtaactttac cagtatttaa acaatgttta tctcaagcta tattacttaa aaagcaagaa
     7381 ttacaagcaa aatctctaaa agcacttgat gacaaaacta atgaattatt gcttagaaat
     7441 gcacaaaatg taagtactca aagtgcacaa atagctagaa tggctggaac aagttctgtt
     7501 caaatagaaa cattggaaaa aatgtacaat actatcaaat ctggtataga tgaaactatg
     7561 agaatagaag aaaacaatag agctttaatt aaggataata caaagagatt ggaagaatta
     7621 aatacgacta taatttataa taagtagtat tatattaagg gctaatttgt ataaagttgg
     7681 ccctttctat gtatatgagg aggctagaca ttgaaatatt tcgattatat ctgcaaagat
     7741 gatttggaga gaattttttt gaaggagcca gaagatttta gtgctaagac tgaaaaagat
     7801 gtgttgaaat atgcactggg cgctttttta tacgttccag ctactcaata taatatgatt
     7861 tataagagta ttatagggga tgttaaggga gttagaccat tagctatttg tttagaagat
     7921 gctgttggag taaatggaga gttagaggct atagaaaatc ttagattaat tttaaaaaat
     7981 attagcaatg aaagtataac taataaagat ggaataccac taatttttgt aagaataaaa
     8041 gatgtggagc aactgttgag aattaaagag attataatta aaaatagaca ttctataaca
     8101 ggaatattaa ttcctaaagc aaattcggag ttaatagaaa attgtataga agctttagat
     8161 tctatgaatc ttcaagatat gtatgttatt ccgataatag agacaaaaga atttatatat
     8221 aatgaaaaaa aagaacttag ttttactaat ttgtacaatg ctattttaag acataagtca
     8281 agaatattaa gcattagagt aggcataact gatatattag ggatgtatgg tataaggaga
     8341 gataaaaact tctctatata taataatttg atttgttctt catttatatt agatataatc
     8401 acatatcttc aaagaccaga gttagatata cctataagtg gtggagtatc agaatttttt
     8461 gatatgacga ataaggatat aagaaataag tatatagaag aaatattatt ggataaatat
     8521 catggattaa ttggaaagac cgtaattcat ccaatgcaaa ttcaaattgt acaagcgcta
     8581 tctacagtat catatgaaga ttttgctgat gctttggata tactggatag tacagacagt
     8641 aagtatggtg tgagcaaggg tgtattagga gagagaatga atgaaacaaa tccacatttt
     8701 ttatgggcta aaaaaacatt gattctatct aaaatatatg gtgtattaaa taaaggagta
     8761 gactatgaag aattgcttaa attttaattg tgagattgac attattgata atccattgaa
     8821 tttagaacta aatgattttt tagacgtagg aataagaaat aatagcaaaa gacgtttttt
     8881 gtttatctca aagaaactcg gtaaacattt agcatgtaag ccaagtgaga tggataattt
     8941 gggaaaatta atggctacaa tttataatga aaaaaataag gaagaatcaa atggcactgt
     9001 tatagctttt gctgaaactg gtacagctgt tgggcatagt ttttttgatt acttatgtgg
     9061 agattatgaa tttattcata ctactagaga gcagtttgac gagttagaaa atctggaatt
     9121 tttagaggaa cattcccatg caacagacca gaatttatac tttggtatgc ttaacaactt
     9181 taaatttgga gatgagataa tattggttga tgatgagatt accacagcca atacatgcat
     9241 gaatataatt aggaaaattc aaagcattta tcctaaaaag aaatacacta tatgctcaat
     9301 actcaattgg gtaagtgatg aaaatttaag taaagttact cgtttagaaa gagagctaga
     9361 ttgtaagata gattttgtat acttatttaa aggaaacttt aaattttata tagatgaaga
     9421 tattatttca aattacaacg ataattatag tattactaaa ttagagacta tttcaagtga
     9481 taaagtaaat ttagtagtaa atcatattta tgttgattta gaagattata ttggaaataa
     9541 gaaatatgta aaatatacag gaagatttgg aattaataga agagaacaat ttagattaaa
     9601 agaaatcgtt aagagagaaa gtaaaaaaat aactatggcc agtaaagaat ctttaaaaaa
     9661 tgaaaaagaa aagattctat ttttaggtac tgaagaattt atgtatatac ctatgctgtt
     9721 tgcaaaagaa aaagaaaatc aatatgatat atactatcat tcaacaacaa gaagtccaat
     9781 tattaatatt gataagaata attatcctat taaaagtaaa tttatgctaa agagttttta
     9841 taatatagat attcaaaatt atatttacaa tatagataaa cataattttt ctaagtgtta
     9901 tttatttgta gagctgaata aaagtgaaaa ttcttatcta gaatttattg atataattaa
     9961 aaaaacaagt ataaaagaat taactatagt atgttgtagc taaagtgttt taacaggata
    10021 aaatatatca cgattttaag ataggagttg agtcatatga gagattttag cacgtattct
    10081 aaagatgatg ttattttttt attaaaagat atatcaaatt taattgaaga agaaggaaat
    10141 aaagaaagag aattaaaaat acaaaatggt agacattatt cagaaatgat accaatagag
    10201 tatgaagtta gtgaagatta tctgaattta tatcatgaga aactaagaga aaataaagat
    10261 aaacttgcat tttctatagg agttatgtgt gaaaaaataa ttaaaaaaaa tggaaatgat
    10321 gtagtactag tttcacttgc tagagcagga acacctattg gtattttggc aaaaagatat
    10381 ataagaagca agtataatat aagtttacct cactatacaa tctctataat aagagataag
    10441 ggaatagata tgaatgcaat tgaatatata gtaaagaatc atccgagctc aaaaattcaa
    10501 tttttagatg gatggactgg taaaggaact atatcaaaag aacttgaaaa agcatgtgat
    10561 gaacttaatg ttaaatttaa aaaacatttt gactcaacac ttgctgtatt agctgaccct
    10621 gctggatatt caggacttta tggaattaga gaagatttct taataccaag tgcatgttta
    10681 aattcaaccg tttctggttt agtaagtaga actgtactaa gagaggattt aattggtaaa
    10741 aatgactttc atggagctaa gttttataga catttaaaag ataaagatga atctatgaac
    10801 tatatcgaaa ctatagaaga atgttttaaa aatcaattta agaatatatc tgatgaagtt
    10861 gaaaactggg aaagtgacat tataacaaga gatggatact ttgatgtttt aaatataaaa
    10921 gaaaagtata acataacaga tattaacttt atcaaaccag gtgtagggga aacgacaagg
    10981 gttttactta gaagagttcc gtataagatt cttgttaaag acttaaatga taaatcttta
    11041 gaccatatat tcatattagc taaggagaaa aatgttgaag ttgaacaaat ggatttaaaa
    11101 gcctataaat gttgtggaat aataaaaaat atgaaagata tttaggaaat atattagaca
    11161 ggagaatatt gaatgattgt tttttcagat ttagacagga gtatcattta ctccaataaa
    11221 tttttaaatg ctgatagtaa atatgctaat atagaaattt atagggaaaa agaaatatct
    11281 tatatatctt tagatactat aaatttaata aagcaaatac aatattatgg tatgtttata
    11341 cctacaacta ctagaacagt ggagcaattt aagcgtatag aatttaataa atatgggatt
    11401 tattttcctt ggagtataac ttcaaatgga ggagttatac ttaaagataa tgaaatattg
    11461 aaatcatgga gtgaaaaaat agataaacta aaatctgatt atgagcccat tgaaagtatg
    11521 attcataaat ttaaagacta tttgaatgta gatggaataa ctaattttaa agttgcagaa
    11581 gatacatttt tttatatagt tgttgattta tcaagattta atttggattc aatcaaagaa
    11641 tatacaaata tattggaatc aaaaaattgg aagttttatg tgagtggcag aaaaatatat
    11701 tttataccaa aagaaatttc aaaagaaaat gctataaaat acttgactaa agaattagga
    11761 atagaatatt tttatgcagt tggagattct attatggact atggtatgct taatatctca
    11821 aataaatcct atgtattgaa gcatggtgat ataaataaaa atgaaataga aaattcattt
    11881 atatcaagtt ctttcaatgg aatgagtgga actgaagaga tactatctaa cattttagac
    11941 gagaactgcc tacttaatat ataatgactg atatataaat aataaactag aaaaatctga
    12001 aataaattat agtaaagttt atcatactaa tttatttcag atttttatca atttatattt
    12061 aagtaagaac tcaatatgtt aaaatgaagg tggagtcatt gcccagactg agatacatac
    12121 ctcatcatca taattttcat acacatgtgg tatagtactg ttaaaataaa aactatctcc
    12181 ttcctccaat agatattcct tatttccaag tgttatcttc attttacctt ttattatata
    12241 gccacattct tctccttcgt gtgtcacaaa ttcactgttt ttgtgtgtat gaccatctag
    12301 cgtaacaagt ataaattcta tttttttccc agataaatta ggaacaagaa gttcgtaaaa
    12361 ttttgtagaa tcattagtta ctatcttttt tcgattattt ttctttacta ctatatcaga
    12421 gtcctctctt ccaatctctt caaaaaaata acctatattt atatcaagag cttttgatat
    12481 tttccacatt gctacaatag atggaacaac catattacgc tcaatttgac ttattaagcc
    12541 tgtacttaaa tctgaatttt ttgctaaagt agagatactt atatttttag attttctaag
    12601 agatctaatt ttgcttccga catctatatt cattagatac aaaccccctc agacatgatt
    12661 ataacatata atttataaag attaataaaa taaaaaaaag ttttataatg catgtatttt
    12721 ttatgttata ttgaattttc tgaaaagatg cattataata gtattaatat tcaatatatt
    12781 gaatattgat aatgatattt taatttaatg aaatggaggt agttaagatg aagagttttg
    12841 taagccctga taacatttgg gtattatggg caattgtaac tggatgggca gcatttagta
    12901 tttacttgga acaaaagtac aattgggcat caaaagtgtc tggagcaata atagctcttg
    12961 taggagcaat gttattatca aatctaaata ttataccagt agagtctgtt gtatatgacc
    13021 aagtatgggg atatgttgtt cctttagcca tagctttatt gttatatcaa tgtaatatta
    13081 aaaagatatg gaaagaaagt ggaagattgc tcattatatt tcttgtaggt tctgtaggaa
    13141 ctgtacttgg agctatgatt ggatttttag cattaaaaaa tgtagtacca gatttaaata
    13201 ttgttgcagc tatgatgaca ggttcttata taggtggaaa tgtaaacttt gctgctatgt
    13261 caggggcgtt tgatgcgcca ggagaattag tatcagcaac agttgtagca gataatcttt
    13321 taatggcatt atatttcttt gtcttaatag ctataccatc aattggattt tttagaaaac
    13381 attttaaaca tccacatgta gatgaaatgg aaagtatagg tataactgaa ggagaaacag
    13441 tagcagcaaa ttattgggga agaaaagaaa tatctttaaa agatatagca atagccattg
    13501 gtagcgcttt tataattgtt gcagtatctg tagaactatc aacttggttt aaagctataa
    13561 taccattatc aaatcctttt ttagcaatgt taaatacttt atttggaaat caatatctaa
    13621 taataactac tttgactatg ttgtttgcca catttatgcc taatttcttt ggtgatataa
    13681 aaggagcaca ggaattgggt acatttttaa tatacatatt ctttgttgta gtgggtgttc
    13741 ctgcttcaat aacattaata attcaaaaat caccattact acttttatac tgcggaataa
    13801 tggtactaat aaacatgcta gttacgttta tagttgcaaa aatatttaag tttagcttag
    13861 aagaagctat tcttgcatct aatgcaaata ttggtggacc tacaacagca gcagctatgg
    13921 caatatcaaa aggatggtct aagctagtag gaccaatact tatagttggt acatttggat
    13981 acatagttgg taattatttt ggtcttttag taggaaatat tttaatataa ttttacgtga
    14041 aaaaaagttg ttttttagaa ggattttatt taattggagg ttgttatgat tttcgatgga
    14101 cattcagata cttgggcaga tgtaacaata agaaggctta acggggaaaa agatgtattt
    14161 agaagaatcc atcttgataa ttttaaaaga gcaaatatag aaggagctat ttttgtaata
    14221 tggatagacc caccatatga taaagagcct gaaaaaagag ttaaacaaat tataagttct
    14281 ataaaagatg aaattgaaga tatgaaagat gttgtaaaca tagtaaaaaa atattcagat
    14341 atagataaag gtataagtga aaacaaaata aatattttaa ttggtcttga aggattaagc
    14401 catattggaa gaaatataga tttattagat tatttttata aagaagttgg tgcaagacat
    14461 gcatcattaa cttggaatga acagaatgaa cttgctacag gatgtttagg ggatgaaaat
    14521 cgtggtttaa ctgaaatagg taaaaaagca gtcaataaaa tggaaaaatt gggcatgata
    14581 gtagatgtgt cacatttaaa tgaaaaatca ttttgggatt taatgtctgt tgcaagtaag
    14641 ccagttatag cttctcattc taatgctagg agtttatgtg atgctaaaag aaatcttaca
    14701 gataatcaat taaaagaaat ttcaaatata ggtggattag ttggaataaa tagttttaga
    14761 ggatttacta atagtaatcc taagaagcaa gatttaatac atttggttga ccatatagac
    14821 tatatggtaa atcttatagg tatagaacat gtatcttttg gttttgattt tagtgattac
    14881 ttagggaaag agactatagg ttcttttact ggaaatgcta gtgtgccaag tgtagatggt
    14941 cttgaaagca cacatacagc acagaacata gtagtagagc ttgaaaatag aggttataaa
    15001 aagaaagata tagagttaat ctctagtggt aatttttata ggctctttaa agatatacta
    15061 aaataatata ataaagaact taaatttata aaatagtata tttatacaaa taatatctac
    15121 atatttaaat tggcgttcat aatttattaa attaaatttt aatgttacta gaagtttcaa
    15181 ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa tgataattat
    15241 tattgataaa ctattaaagt gaaaatgata ataattttca ctttaattaa taaaagattt
    15301 ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt tgtcttattt
    15361 ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca aaaatcagca
    15421 taaacttatg atatttatca tgagtagaat accaagatta ataagtatat tgattgctgg
    15481 tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata aatttgtatc
    15541 acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat gtatgctttt
    15601 agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt ctcttgtagg
    15661 gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa tctttgtacc
    15721 attagtaggt ataatgtttg gaaatataat aggttctatg acggatttta tcgcgtataa
    15781 atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta tgatacttaa
    15841 aggaaattat gagatattat atatcacaat accccttata atattagcat atatttatgc
    15901 aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag gtcttagtta
    15961 caaaagaatt gtcaatatag gtctgataat agtagcactt tttacagtat gtgtagtcgt
    16021 tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat ccttgtatat
    16081 gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga tatttgtact
    16141 tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta tagggcttac
    16201 agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga atgtaaatga
    16261 agcttagtct caatcaaaaa atttactttg taagtatatt actattcata tcaatagcac
    16321 tttttataat ttatggaata gacatgaatc atttagaata tgcattatca caaagaatac
    16381 caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact gtagtgtttc
    16441 aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat tcattgtatg
    16501 taatgattca aactataata gtattcatat ttggttcaag cagtagtctg attataaatg
    16561 aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg ttattatata
    16621 agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta ggaatgatat
    16681 ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagat ccgaatgaat
    16741 ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact aatgtattgt
    16801 taatagcatt tataataatt attgccataa taccttttat atatgatgaa ataaagtatt
    16861 tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat tttgataaag
    16921 cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca gcacttattg
    16981 gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt tttaaaacat
    17041 ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca ttgataatag
    17101 gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta gtaataaatt
    17161 ttataggagg aatttacttt attcaattac tattaaaggg ggcaaataga tgatagagat
    17221 aaaaatatat ttaaaagata taagaataaa aatgttgtag atgatgtctc ttttagtata
    17281 gaaaagggaa agataacatc ttttatagga ccaaatggag ctggaaaaag tacagtacta
    17341 tcaatagtta caagacttat aggaggtgat ggtggagaag taataataga aggaaaaagc
    17401 ttgactaatt atagcaataa agaattagca aaaaaaatag ctattcttaa acaatctaac
    17461 aatataactt taaagcttac aataagagaa ttagttggat ttggaagatt tccatatagt
    17521 gaaggtaatt taactaaaga ggatgaaaat tatattgatg aagcaataga atatatgaag
    17581 ttgactgata tacaacataa gtatttagat gaattgagtg gaggtcaaag acaaagagct
    17641 tatatagcaa tggtaatagc tcaagataca gagtacatat tacttgatga accactgaac
    17701 aatttagata tgaaccattc cgtacagatg atgaaggtac ttagaagtct ttgtgatgag
    17761 ttagataaga ctatagtatt agtaatgcat gatataaatt ttgcatcatg ttattcagat
    17821 aatatagttg cattaaaaaa tggaaaagtt gaaaaagtag gtcgtacaga tgagatagta
    17881 aatgaaaaag ttttagaaga tatttatgag atgaatttta acattaagaa tataaatgga
    17941 aatcgaatct gtatttattt ttaaaatatt gtatatgaaa ataaacgtgc tatatgattt
    18001 tttataaaat ttttaaatta aaataaagat taataggaga gaggaaaata ttgatatgaa
    18061 taaaaaagca gcaatagtag cagcagtagc tattatagga ttagtggcag tgtttgcatt
    18121 aggtggaagt aaaaagaatg aatcaaaaac atcagaaaat tcaaataata ccatgaagat
    18181 aactcataac ttgggagaaa cagatgtaaa attaaatcct aaaaaagtag tggtatttga
    18241 ttattcggct ttagatacaa tggatgcatt aggtgtagct gagaatttag taggacttcc
    18301 aaaagcgagt ttaccagcat ccttagaaaa atataaggat gacaagtatg cagatttagg
    18361 aggattaaaa gaaccagatt tagagggtat aaagtcggca aatccagatt taattataat
    18421 aaatggaaga caagaagatt tttatgagca attgtcaaaa atagcaccaa caataagtac
    18481 aagtaaagat gataagaagt acttagaatc tgttaaaaat aatatagaca aaatagcaaa
    18541 aatatttgga gtagaagaaa aagcaaatca agaatttagt aaaattgaaa agaaaataga
    18601 aactttgaat aaaaaagtaa cagataaaaa tttaaatgct ttaactataa tggtaaatga
    18661 aggtaattta agtgtatttg gtgaagaatc aagatttagt atattataca atagttttgg
    18721 atttgaaaat aaagataaaa atataaaaga atcaagtcat ggacaaaata ttacttttga
    18781 atatatagct aaacaaaatc cagaagttat gttcgttata gatagaggaa tagctactgg
    18841 cagtgatgta aaagaaagct caactgcaaa atctgttttg aacaatgata taataaaatc
    18901 tatggatgca tataagaatg ataatataat atatttagat tcaccaacat ggtatgtaaa
    18961 tgatggtggt ctaacatctt taaataaaat gatagatgat gcttcaaaag cggtaaatta
    19021 ataatccgat ataatcaaaa tgtatttttt ataaaatgat tatcaaataa actaggagtt
    19081 tttaagaact cctagtttat tttttgcata aaaaagatgt agtttaaaca ataatattat
    19141 ctataataag ataaatctat tcaaaatcta ataaaatatg aagttatact agacaaaaag
    19201 aataactctt aaatataatt aagttatata ataatatata attataatga tataatttta
    19261 ataggtggtt tgtaattaaa aagttt
//
LOCUS       DAEMHZ010000067        19071 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000067 DAEMHZ010000000
VERSION     DAEMHZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.067"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..668)
                     /locus_tag="KPK72_002231"
     CDS             complement(<1..668)
                     /locus_tag="KPK72_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489443.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTN"
     gene            complement(669..1421)
                     /locus_tag="KPK72_002232"
     CDS             complement(669..1421)
                     /locus_tag="KPK72_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489444.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(1737..2891)
                     /locus_tag="KPK72_002233"
     CDS             complement(1737..2891)
                     /locus_tag="KPK72_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2489445.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(2894..3361)
                     /locus_tag="KPK72_002234"
     CDS             complement(2894..3361)
                     /locus_tag="KPK72_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2489446.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(3375..3863)
                     /locus_tag="KPK72_002235"
     CDS             complement(3375..3863)
                     /locus_tag="KPK72_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489447.1"
                     /translation="MITNKIKNIIAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(3877..5208)
                     /locus_tag="KPK72_002236"
     CDS             complement(3877..5208)
                     /locus_tag="KPK72_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2489448.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(5222..6787)
                     /locus_tag="KPK72_002237"
     CDS             complement(5222..6787)
                     /locus_tag="KPK72_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2489449.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(6956..7741)
                     /locus_tag="KPK72_002238"
     CDS             complement(6956..7741)
                     /locus_tag="KPK72_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2489450.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(7908..9107)
                     /locus_tag="KPK72_002239"
     CDS             complement(7908..9107)
                     /locus_tag="KPK72_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG2489451.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDDEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(9629..10966)
                     /gene="xylA"
                     /locus_tag="KPK72_002240"
     CDS             complement(9629..10966)
                     /gene="xylA"
                     /locus_tag="KPK72_002240"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG2489452.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIRLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(11062..12612)
                     /gene="xylB"
                     /locus_tag="KPK72_002241"
     CDS             complement(11062..12612)
                     /gene="xylB"
                     /locus_tag="KPK72_002241"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG2489453.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKEDFKLLDE
                     VVNAKIND"
     gene            12820..13980
                     /locus_tag="KPK72_002242"
     CDS             12820..13980
                     /locus_tag="KPK72_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2489454.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(14136..14540)
                     /locus_tag="KPK72_002243"
     CDS             complement(14136..14540)
                     /locus_tag="KPK72_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2489455.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMIEQNASLLSDDE"
     gene            complement(14561..15043)
                     /locus_tag="KPK72_002244"
     CDS             complement(14561..15043)
                     /locus_tag="KPK72_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2489456.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(15063..15872)
                     /locus_tag="KPK72_002245"
     CDS             complement(15063..15872)
                     /locus_tag="KPK72_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2489457.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(15865..16644)
                     /locus_tag="KPK72_002246"
     CDS             complement(15865..16644)
                     /locus_tag="KPK72_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2489458.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(16644..18722)
                     /locus_tag="KPK72_002247"
     CDS             complement(16644..18722)
                     /locus_tag="KPK72_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG2489459.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT         6366 a         3374 c         2076 g         7255 t
ORIGIN      
        1 atattggtag aaagatagta gccatatcct attactataa ttgatactat agctaatata
       61 tatataccta ttcctacctt tggtattttt tcttgttttt tagatatatt gattagattt
      121 attacactac ttctacatat tgttataaaa ccttttacag ccattattat aaatatagaa
      181 ataaagatta aagtagcata tagtatagct tctatactaa gtccattaga tacctttata
      241 ttaaatccag ataactttat tatcactact tgaaaaaatc tagatgttat tatccctata
      301 attattcctg ttatacaaga acttattcct ataagcatat tttctacaaa atatattttt
      361 cctatagtat ataaatctaa tcccataaaa gacaatattc caatttcttt agacctattt
      421 ttaaaaaata tatttgttgc ataccatata aagaaaaata aaaatactga tagtatcatt
      481 attacagcta ctaacactga tagtgtaaat tttttatttt catcttgtaa cacatttata
      541 gcatcaccat atcttaataa ttcaaaatta gttagcacaa ctactgaaaa aaccatagat
      601 ataaaaaatg ctagataagt tcttatgtta ctcttaaaat tattataagc aattccaagt
      661 atactcactt acatctcacc tcccatagag gctagcatat ccataatttt tccataaaat
      721 tcttttctac tatttccttt gtttaattta cactttatat ttccatcatt tatcatatat
      781 acttcatttg aataacttgc tgttagaggg tcatgtgtaa tcattataat agtagcgtta
      841 ttttctctgt ttattttttc taatgattct aaaagttcat atgctgattt agagtctaaa
      901 gcaccagttg gctcatcagc aaatatgact gttggatttg taattaatga cctagctgct
      961 gcgcctcttt gtttttgtcc tcctgataac tgatatggat atttatctaa atgattttct
     1021 aatccaaatt ttttagctat agaaaatacc ttagcttcta tctccttact tttctttttt
     1081 cctaatgcta gaggaagtgc tatattgtcc attagtttca tattgtctaa tagattatag
     1141 tcttgaaata taaaccctat tttttctctt cttaattttg atagttcatt attttttact
     1201 tttgatatat taattccatc tagtattatt tctcctgaag tttgtttgtc aagtgtagac
     1261 attaaattta gcaatgttgt ctttcctgca ccagatggac ccattattga tataaaatca
     1321 ccttcattga ctgtaagaga tatatttttt aatacatttg ttttaaatcc ttttattcca
     1381 taatcttttg atatattttt tatttttaat atttctttca taataatagc ctccagttgc
     1441 taatttttta atatatattt attttaaagg ttttatataa ataaatcctt aagtaaatat
     1501 tacattttaa ctgttagcat tacacttttg taacatatgg aagactatta taattataat
     1561 ggaaatatct taaaataaaa gcatgattta cttctttgta taaacaaaga aaagaagctc
     1621 ctaagaagcc tctaatctat acaattattc ttctctataa aaaaccaaaa tacacattga
     1681 ttgtggcaaa tacagatact ataaacaagt gatattacta ctatatatta aaacaattat
     1741 tcctgaagat aattcaataa gactatcaag caataagcta agcatccaaa aagacttaat
     1801 accattaatg tataattata cccaatatta atgctcaaaa aaccagcaac agttggaata
     1861 atcagagatg aacctcccat aaatatactt aaagctatta attggctact acttctaagt
     1921 tttttaggag aaacttcaaa tattaatctt tttgaagtaa ttaaaattaa aggagtagaa
     1981 aacacttgaa aaacacacag tataataatg atatttgaat catttgttaa agcaaataat
     2041 agaaactgaa ctgttatcat aatagcactt aattttaata agtttaaact gctatatttt
     2101 tttaataaat aagaaccagc cagatatgtt ggtatctcta acaatgactg taaagaccac
     2161 tttaaagata tctgagaatt tgtagcattt aaagctatca ttttatctat tacagtacag
     2221 ttatttgcta ccaccattga gtataataag aataaaatac atactagtaa gatatatttt
     2281 ttatctttta ataattcaat aatatcttta ttagttacat ttattttctt attaatcttt
     2341 tcaatatcag gtaataatag tatatttata ataagtaata ctacaattaa accaatcgct
     2401 acagataatc caaaatatga gaatctaaga atcaagtaag atgctaccat acttccaata
     2461 gcccatccaa tcgacccaaa agtcttaata aatgacaaat aattattaat attatctcca
     2521 caacctatta tccatgtatc atataaacta cagcaagtat ttaataatcc tcctgataaa
     2581 gcaacaagta ataagtatat tataaaagtt gcagattcag tagaaaacat aataactgat
     2641 gcaattccat aaactgataa actaattaaa acaatttttt tcattgtttg atacttatca
     2701 gctaatactc caaataataa ttgaaacaat atattggtta ttgcatagct cgataatata
     2761 attcctcttt caaacgctga gtaacctaat tttgataaga aaggtgtgaa ttttgttgaa
     2821 gcaaaggcaa aaataatata tgagaaaaaa tagaatatga taaatttaaa tttaatcgct
     2881 ttattaatca taatcaatca aaattgataa ctttaatacc actatttcct tcctgattta
     2941 agtttgaaag atgatatttt accgtattag gattctctac aataactaaa ggaatatata
     3001 aagtattttc aagtaactta ttatcaattt ctactaactt ttcccccata ttaacttctt
     3061 gaccttgttt aacttctaat ttaaagtttt ctttttttgt tgttgcacta tccaatccaa
     3121 tatgaataag aatttgaaca tcattttgca atttaattcc tatagcatgg ttagtttgag
     3181 caataaaact tattactcct ttagtcggtg atttaagaat attgtccttt gctatcatag
     3241 caaatccatc tcccattacc ttagttgaaa atactttatc aggaatttct ccaagattaa
     3301 ttatttttcc atcaatgtat gaattaattg taattgcttc tttttttctt ttaaagaaca
     3361 ttatagacac tcctctacat aattaacttt attctaatta tcattacaaa taacgatatt
     3421 aagcatactg caattgagga aaaaagagcc ttatagctct ttccccattg tagttcttta
     3481 atactgctta tgatttgttt aattccaaca aatttataaa tataggtaaa catgataaca
     3541 atagttattg tgtaaatttg gaatatctgc cctaataaga ctaaatagaa agcaatcatg
     3601 ttttctttta tctctttatt cttttttata ttgtaaataa catatgcact gtatactgca
     3661 ctaaagttag agattaaatt taaacttgca ttaacatcat tgtttaaaag ttgtgaaaat
     3721 gaaatgtttg taaataatga tgtcaattct aaatacacta tatataaaac aggtaatatt
     3781 agaaaacaaa ccataaccac tgaaagaaat atattttcat cttttaatac tgctatgata
     3841 ttttttatct tatttgttat catgtttttg ttctccttat ttaaaagttg gccagaaatc
     3901 cttattagcc acaatcattt catctaagat ttcttttgct acttttgcac ttggaacagt
     3961 tttactcata gtaaacgact gccataattt ttgataactt ttttctatcc atgcttctac
     4021 agctaatttt tcagaagcaa cttgttgttc cattaaacct ttttggaaag ttggaatttt
     4081 acctatagac ataggttcaa ccatatcttt tccaacaagg caaggaattt caaccattgc
     4141 atcatctgca aaattttcaa tagctccatt attaggaaca atcagtaaaa atctttcctg
     4201 agtattaaat gctaatgcac aagctaattc aacaataaat tgagaatgct cacttgcatc
     4261 aaaccatgta tcccttgctg tgccattttt tataattctt tcacattctt cgaatgtatc
     4321 cttttctctt ctatccataa tttcatttgc tcttgtataa tttggattag catgttcaac
     4381 agtttcatca gccattaaat aatattttaa ataagtatta ggtaatgtag ttggttctat
     4441 ttctaataag tcttttgctg ctctcatagt ttctaaccaa ctagattctt tgtgttgacc
     4501 ttgaggtgta aattcctcat atcctttttt agcacaatat tcttgtaatt taggcattaa
     4561 atcatttcct tctttgtcct taatacttgt ccaccacccg aaatgattca atccataata
     4621 tctaacatca aagtctttac gagaatttaa acctaaaact ctagcaaata tttcctctaa
     4681 acatactggc atatcacaaa tattaataat tcttgaattt ggtcttaata cacgacatgc
     4741 ttctgcaact atagctgctg gatttgaata attaagcatc caagcatttg gagaatattt
     4801 ttccatataa tcaatcattt cgattattgg acctattgtt cttaatccat aggcaattcc
     4861 acctgcccca caagtttctt gaccaacaac cccatatttt agtggaattt tttcatcttg
     4921 ttcacgtaaa gcatataaac caacacgaat ttgtgccatt acaaaatcaa tatcagtaaa
     4981 tgcttcttct ggatttgttg tataactaaa tttaatttct ggatttttct ctctaacaat
     5041 tattgcacat gcttcagcaa cttttctttg tctctcatca ttattatcat ataatttaat
     5101 tgattcaata ggaaatttag caacactttc aattaacatt gttacaattc cagcagtata
     5161 agtactaccc ccaccagcaa ttagtatcct ctgtcttttt ctttccatct ttccttctcc
     5221 tttataataa attttcaaat tcttctctta cacttggtac tgaaagccca ataataatct
     5281 gaatagcttt tccattatgc actaaaccat gtgcacctac tgccttaaaa tcttcttctt
     5341 tagaaataat tgagtcatct ttcaaggtta atctcaacct cgtagcacag tttgtaacat
     5401 ctacaacatt ttctgctcca ccaactaatt ttatgtattc agatgcttgt tgagaggctt
     5461 tactcaattt atttcctttt tcatctttag tttctaagta ttcattttta gtatatagtt
     5521 ttgtttcttc actatcttcc tctctaccag gagttttcat atcaaacttc ttaattaaga
     5581 aagtaaatac aaagaaccag ataaatgaaa atataatccc tacaacaact tgaacaacat
     5641 ataccttcca gtgatacatt ccaagaggaa gccaatttaa tacaacccag ttaataattc
     5701 ctccaccaaa atcaccaaca actcctaggg caaacgaaat cgtttgcaag catgcatcta
     5761 aaaatgcatg tactaggaat aaaagtggtg ctacaaataa aaatgtaaat tctaacggct
     5821 cagtaattcc agttaatact gcagtaagag tagcaggaat cattaaacct aatacttttt
     5881 tctttttttc tggctttgca gttacataga aagcaccaaa tacacctaat gaaccaaaca
     5941 ccttcgataa tccggataat gaaaatccga ttggatacat ttcctttaga gtcttagctg
     6001 aagtttgaaa atcctgaata tgagtagccc aatatactga tgtaccacct ggaacaacaa
     6061 cactatcata acaaattggt gcatatacaa aatgatgtaa tccagttggt attaaaatct
     6121 tttgtaagaa tgcataacac caaactccta tatttccact atttataaaa aacgtttgca
     6181 aatgtctaat aacattttga atatgaggcc aaataaaaca agttaataat gcaactggta
     6241 tcataacaaa gaaacaaatt gcaactacaa gtgatgagcc tctaaataat ccaataaaat
     6301 ctggcagttc tttatcataa aatctattat gaatataaat gacaatacct gatattaaca
     6361 aagcaccaac cataccagta tctaatgttt taataccagc aatgcttaca agtccacttc
     6421 cagcacttat ttcagctgca aaatcaactc caaagctttt tccccaaaaa cttaacatag
     6481 aacctacaaa ataattaaaa gtcaaatacg ttgctaaagc ctccatacaa gctcttgctt
     6541 gttgcttttt tgcaagtgct attggtaaag aaatagcaaa taataatggt aattgattaa
     6601 ataccatcca agctccacta gatattacac cccaaaattg gtaccaaata ttttctggtg
     6661 tagcaagtga acccataatt gactgatttt gaaatacaat tgagaatcca accattatcc
     6721 cagcaaaagc aaataatagt gttggtgtaa acattgcccc tccaaaacgc tgtattttct
     6781 tcatcatata aattactccc ctcctcatat ttttagttta tctttggcct tagatatcta
     6841 ttattttaac aggttaataa atatatacaa tctttagggt acattcggag cttattcaca
     6901 gtcaaataag tttttgcaca tttttatgtt caaattctca taaaaaaaag aatgactaca
     6961 atttgcaatc aatcccttct gtaatcattc tatgcctttt ataaatacta tatttaataa
     7021 ataataactc aatcaatata aaatataaca ttgtcaattt ataactaggg tgcatttgta
     7081 aaaatgagat attagtcgct gaaatataaa ttgattcatc acttaaatca gtcaatgtat
     7141 tatttttaaa ctctgtaatt gaaattattt tagttcctat taattttaat cttctagcaa
     7201 tattaagtgt attctcattc tcaccactta gggatataat aatcataaca tcatttttac
     7261 tgacaatatt gtaaaaagaa tcatcaaatg ttcttccctc aaaatcatat attatttctt
     7321 gacatgataa gaacattctc tttatctgtt ttgcaactac attttgaata tcacctgaac
     7381 cataagcgta tattgttctt gattcataaa gaagtttaca caatgcatca taatctttat
     7441 ttttttgatc ttcaacatac ttaattattg aattacatgc aatatcaaat gactgatttt
     7501 ctatgacatg aaccttttct gattcccagc ttaataatac tttaaactca ctgaaacctt
     7561 ttaaatctaa ttttttaaca aatcttgtaa tagttgcacg agacacatta catttcatag
     7621 caagttcatt tattgacatc ttactacaat gttttttatt tgcagttata tatttccata
     7681 ttaataaatc tgtatcattc aatttattat aatttaaaaa aatcatttct tctaatctca
     7741 taaactatta ccccctttaa ttaaaaatcg tcataaatat tataccaata ttcaacatta
     7801 aattacagcg tattcttata tttactattt agcccataaa attgactata aaaaatactg
     7861 ctataattga tgtttaaaaa aatgtagttt taattttatt cttttatcta ataattaata
     7921 ttttcaagtt ctagtgcttt tcttatacaa ttatcatctt taaaatcagc agtcctttta
     7981 caatctttta ctttaaattc tggaatagtt ttaaattcct catcacatat tccattctta
     8041 tttaaataca tacaacttgc cattctaact attagaccac tattttttaa attaaacaat
     8101 acagggtcta tacaccctcc atctccgcca gtagtttcac caactaaagt cgcaaattta
     8161 gtttctttac aaaacattgc aaatgactct gatgatgaat atatttttct atcaacaagt
     8221 aagtatatat tacccttaaa ttttgaatca tctgtagatt ttatagtata ggaagtatct
     8281 tcaaaatcat taaaatcttt tataatttct ttaggtgcat tttcctttac acttgttggt
     8341 aaattttcta caggatttaa ttttatattt cttgcatttg tatattgttt tactatttca
     8401 ttattatttc tataaattct ataaccattt attttgacat cattttttat gagtttagat
     8461 acgattccct gccaataact atcacttcca cctaaattac ctcttatatc aataattaaa
     8521 gctttatgtt tttctaaagt gtcaatataa tctccaatta tttttaaatc attatttata
     8581 gagccattct ttgatgacat tgatggtaaa tacatatatc caatctgacc cttaataaca
     8641 tcctttaaaa ttaattcttt ttttacaaaa atatcatctg gcatttttat tttaagattc
     8701 atagagtcat atctatctac aactttttta tcattaagaa aatcatacca atcattatta
     8761 gagtatgatt tcttaaaaag ttcatatctt ttcttattat ctattacttc tgtgtgttta
     8821 ttatttaaat cagataatat agatgacagt tcttctataa attcgtcatc attttcagtg
     8881 ttttttattc gttttgtata ttcatcctta tttgctaacc aatcaatatt attaagcctc
     8941 ttatttacac ctagatatgg atatcctgtc tttacaacat tatacacata attaaagtca
     9001 tccacctttt cctcttcaga caactgattc ttttgagaat aagtagaatt acctaataag
     9061 attataaatg ctaaaataat tactaaaaat aatactttct ttttcatgta attctcatcc
     9121 actttcagat tttatatttt aatacattgt atcattattt tattatcacc taaacagtta
     9181 aaagaaattc ttgaagatat attccactat tattctcttt aaaaattttt atttgtattt
     9241 tcaaaattat ttcatgaaag ttataaaata aaaattatat tcaaatatct attttactgc
     9301 tatatcctga aagtaaattc ttatcagtga tatactaaaa aagatatgct aaaaaattta
     9361 tccattataa tgacttaaat ttagatactt tactgatttt tatttattag ttgagaaaga
     9421 tattgataat taaaaatatt gataactata gaaatgatta ttttttatag aaattttaag
     9481 gatataaact aattatactt aagtctatat ccttaattta tcatgattat gagaattttt
     9541 acaaatactt atctttgtcc gttttagaat aaaaacaaga acatatcctg ttattttatt
     9601 caaacatcca agtttaatta ctacacaatt acttattaga aattgtatct acttcatata
     9661 tatatctgtt aagtatagct tctagcattt cttgtcttcc agacttattc tttataactg
     9721 gcatttgcag tgcataagat tctaactctt taaatccaac cttaccttct actatatcct
     9781 tacctatacc ttcactaaaa cttgcatatc tttctttagt aaagttctca aatacctcgt
     9841 cttctaataa tttatgagct attaataatc cctttgcaaa tgtatccatt cctgcaatat
     9901 aagctaggaa taagtcttct acctcaaaag atgctcttct tactttagag tcaaagttaa
     9961 accctcctgg tgctattcca ccttgcttta acacttcata catagcaagt gttgcatcat
    10021 atatatttgt tgggaattgg tctgtatccc aacctaataa taggtctcct tgatttgcat
    10081 ctatactacc taaaacattg tttattcttg ctatatttaa ttcatgttgg aaagtatgtc
    10141 ctgcaagtgt tgcatggttt gcttctatat tcattttgaa gtatttatcc agattatact
    10201 ttcttaaaaa tcctaaaaca gtagcagtat caaaatcgta ttgatgttta gtaggctctt
    10261 ttggcttagg ctctattaag aattgtccag tgaatcctat ttccttagca taatctacag
    10321 ccatttgtaa gaatcttgca aagttatcca tttcaagttc tgtattagtg ttaagtaaag
    10381 tttcataacc ttctcttcca ccccagaata caaaattttc tcctcctaag tacttagtta
    10441 tttccatagc ttttttaacc tgagctgctg catatgcata tacatctgca ttacatgttg
    10501 tagatgcacc atgaacaaat cttggatttc caaacatatt ggcagttccc cataataacc
    10561 ttatacctgt cttttccata ttaactttta tatactcaac tatttcatct aatatcttat
    10621 ttgtctcttc taaatctctt ccttctggtg ctatatctct gtcatgaaaa cagaaatact
    10681 caattcccat cttagacata aattcaaatc ctgcatcaac tttaatcttt gctatctcaa
    10741 ttgggtctgt tatattattc caaggtctct ctactgtgcc tactccaaat gggtcattac
    10801 cctctccaca taaagtatgc caatagctca ttgcaaatct taagtgttct ttcatagtct
    10861 tattacctac cacttgttca ggattgtagt atctaaatgc taattcattg tcagatttta
    10921 ctccttcaaa ttttatctgt cctattcctt taaatatttc actcatttca tgtcctccta
    10981 atattttaca catataatat atgtagtatg ttttcttaat ttaaaactat tttctatctt
    11041 tatttttctt tttaagctaa tttaatcatt tatttttgca ttaactactt catctaataa
    11101 cttaaagtct tctttcaaag atttgtatat tcttttatac aatctatgat atatattgta
    11161 tgtatctaca ttttcactta ttggttctaa tttagttgaa gtttttatta tagcatcaca
    11221 tgctttttgt atattttcat atattccagc tcctactaat gcaagaattg caactccaag
    11281 tgctggtcct tcacttgaat ttatagtata tacatcttga ttaaacatat ctgcttgcat
    11341 ctgtctccat actttacttt ttccacctcc acctgaggct cttacttcat taacgtctat
    11401 attaagattc tctattatat ccatgcagtc tttaagacta tatgaaacac cttccattat
    11461 tgaccttgca atttcttttt tagaatggat tggagataat ccaaagaaca cacctcttgc
    11521 ataagggtca agatgaggag ttctttctcc catcatatat ggtaagtaaa gcaatccatt
    11581 acatcctgtt ggtactttac ttacttgatt atttataatc tcatatacat cttcacctaa
    11641 agaatttgaa acttccactt cactttgaca gaaattatct ttaaaccatt ttaatgacaa
    11701 gccagctcct tgagtaactc ccataacatg ccatgtattt ggtattgcat gacagaaagt
    11761 atgaactcta ccttctttat caatttttgg ctcttttgta taagcaaata ctacacctga
    11821 tgtacctata gtagatgaaa ctactccatc tttaactatt ccatttccta tagctcctgc
    11881 tgcttggtct ccagctcctc ctaccactat tgttccttct ttaagccctg tcaaacttgc
    11941 tacatctcta gtcacataac ctgtaatctc ttgtgattca taaactttac ctaaaagatt
    12001 tttatctata tttaactttt ctagtacttc atcgctccaa tctctttttc ttatatcgag
    12061 caactgcata ccacttgcat ctgatacctc tgtagcatat tcacctgtca atttgtatct
    12121 tatataatct tttggaagaa gtattttatt agtattttta tatatatctg gttcattgtt
    12181 tctaacccat aatatctttg atgctgtaaa tcctgtaagt gctggattag cagttatttc
    12241 aatcagtctt tcttcaccta ctaaatcagt tatctcctca cactctttta ctgttcgttg
    12301 gtcacaccat attatagagt ttctcaatgt tttaccttca ttgtccagca taactagacc
    12361 atgcatctgc cctgataatc caattccttt tatacattca ctgcttatat ttgaactttg
    12421 aactacttct cttatagatt cgactacagc tttccaccaa tcatttgcat cctgctctgc
    12481 ccaacctgat ttctcttgta taagaggata ttcatatgta caacttttta tagtatttcc
    12541 tagtttatca aataaaactg ttttagtacc tgatgtacca atatccacac ctaatacata
    12601 ctccaacttc atccctcctt gaatgccact tcataactat cgttttccta tcctaatttt
    12661 tttatatttt aggtatattt tacttgcttt atttttgtta gtatattaaa ctaacttatg
    12721 aactaataat aaaatattta attatctttg tcaatgcaaa ataacctttt atttttgtgt
    12781 aaaaagtatt acaatattat tttaaggagg aatatcaaat tggttactga taaatacact
    12841 attcgagaaa tgaatgaaag attggtactt gaacaaatta taaaaaatgg tcctatttct
    12901 agggcaagta tagcttctac tataggattg aataaagcaa ctatatccgc tattaccaaa
    12961 aaacttattg atgaatctct agttcatgaa atcggaattg gaaatagtac tcatagtggc
    13021 ggaagaaaac ccatattatt agtatttaat aaatgtgcag ggatttcttt aagtatggac
    13081 attggatacg actatatatt ttcttctcta tcttacttag atggaactat tataaattct
    13141 aagaaattaa cagatataca agtaagtaaa gacaatgtta tacaattgat agatgaaatt
    13201 ataaatagtt ataacatatc caaaatagac actccttata aggtaattgg tcttactttg
    13261 gctatacatg ggattacttg tgaaaataaa gtattgttta ctccatatta caatcttaat
    13321 gagattgact tatatagcat ccttagcaaa aagtatgact ttcctattca tatagaaaat
    13381 gaagcaaact taacagcttt agctgagaat actttttcaa ctgtacataa ctcactactt
    13441 agtctcagta ttcacagtgg atttggttca ggcattataa taaataataa attatatagt
    13501 ggcagaaatg gtatgagtgg agaaattggt cacactatca tcatgcctaa cggtaaacta
    13561 tgtccttgtg gaaatcgtgg ctgtttagaa cagtattgtt cagaaaaaaa ggtatttgaa
    13621 cagctttctt ctttagaaaa tattcctaaa atagattctg atatagtaaa gcaactatat
    13681 tatgaagata accagaatgc caaaaaagtt attcatgagt tttgtagtta cttaacaata
    13741 gctataaata atgctattac aacttatgct cctgaaataa tatatttaaa tagccaaata
    13801 attagtgata tccctgagat attacagatt acaaaagata tgttagttag ctcttttaac
    13861 aaaggaataa atatagaaat ttcatcatta gggtctgagg catctctata tggtggctct
    13921 gccgtaaata tcaagagctt tttgaatata caaaatttaa ctttaattaa tgaaatataa
    13981 gggttatgac tatatacagc cataaccctt taaattatac attctaaaat tagatttatt
    14041 atatagttta tgcaagatta gctatatttt ttaaaaattg tatatcaatt aagtattaac
    14101 ataagtattt tgttttaaat attaatataa tcttcctact catcatcaga taataaactt
    14161 gcattttgtt caatcataga aatattatct ttacctattt tctctaattt tgctgccaat
    14221 tcctttgtat ctaaaaaagc tgattcaata accataccta aatttagccc tgaaataatt
    14281 ttaattcgac tgtcattatt ggctactaat aaagcagcat tgcatggagt tccacctagt
    14341 aaatctgcca ttataattat ttctgaactt tccttgtaca cttctaaagc tttttttaat
    14401 ttttctattg ttccttctat tccatcatct tgtttcatat ttacagtagg ataatctata
    14461 gaatccccta ttatcatttt tgcagaattt aagacttctt cagccatatt gccatgactc
    14521 atcaaaataa tactattcac tttaatacac ctctttaaat ctataagatg tctacaagat
    14581 aagttttctt atcatccggc gtcattatag ttgtaatttc aattccttca tctaaaagtc
    14641 ttttaaatgc ttctttctct tcttcagtaa cactgatatt aggtcttaga actgttgtat
    14701 cttttctact tgacatattt cctacattta tattatgtat tttaaatcct gcctctacaa
    14761 atggaataag gtccacagga gacttaacaa ttaataatac attttgtcct tcatactttc
    14821 ctgcagtaat attttttata gcagtctctt ctgtaattat agatgacgct atacctgcag
    14881 gtgtagccat tcttaaaact tgtttttgaa cagaattgtt agctactgca tcatttacta
    14941 ccattaatct tgtaacaccc aatttattac tccacatagt tgcaacttga ccatggatta
    15001 aacggtcatc tatacgaata tttttaatac ctttcatttc catatctaat atcatccttt
    15061 ccttatttta aaatacctaa tgcatataaa attattgaaa caaccattac tcctaaaatt
    15121 aagtatatag gttttatatt ctttttaaga gcccaataaa caatcacaac taaaattgca
    15181 ggaactaacc caggcataat ttggtctaat atttcttgac ctttcattgt aaaatcacct
    15241 tgttgaaagt tcaaagcaac attagctttt acaactgaag gtattaatgc acctacaacc
    15301 attaaaccta aaatagatgt actctcagtt aaaatcttta aacttttttc aattgaagat
    15361 attagcttag tacctgatgt ataaccagca ttaaatagtg gtatacttaa taatttcaat
    15421 gctacactca ctacaatcca cagtaagatt ccaaatggac ttccatctat cgccatatta
    15481 gctgctatag ctccaaaaat tgtccacgga ataacgacaa ataaactatc tcctaaacct
    15541 gctactggtc ccatcaagcc tgttttaatt gaagcaacag cctcttcagt ctcttcactt
    15601 gataactcct ccattgctac attaataccc aaaatataag gggcaaccca aggattagtg
    15661 ttaaaaaatt gtaaatgatt taatacagca ctttgtaact tttctggatt atctttgtat
    15721 actactttta aaaaaggtag tactgcataa cagtatgcca aggcctgcat cttctcataa
    15781 tttgaagaaa cagaactagt aaaaaaccag cgccataaga cacttttttt caccttttca
    15841 tttgatttat ttaatgatac cttatcactc atcttcgtcc atacctccta ctacagtata
    15901 ttgttgttct tgattttcca aattcttttt gtaaattata agtgcaattg ctccaccaat
    15961 taaagctaca cctaataatg gtactttcat atatactgct aaaacaaaac caattactaa
    16021 gtaactaaaa tactccttag caggaagata tctaagtaat aatcctatcc ctactgctgg
    16081 aagtagtcct ccagctactg ttaaaccacc tgttaaccat tcaggtgtat atttcaaaat
    16141 ggtattaatt aaagaaggtc caaatattac agatagagca actggaatac ctgttgtagc
    16201 tgctgttaat agtactccta acatttgcat ataggttata tttttataat ttcctttttt
    16261 agctccatct tctgctttat gaactagcca tataccaata gtatttctaa gaacatctaa
    16321 ttgtaccatc aataaagcaa ctggaatacc tatagagatt cctacagatg cttcttgctt
    16381 agtcgtaata gttataaatg tggctactat acttgctgtt tgataatcag gtattgatgc
    16441 tccaccaaag ttactaatac ctaatgataa tagctgtaat gtacctccaa tatataaccc
    16501 tgtatttata tctcctaaga taagaccagt aataaaacct gctgttactg gttgatatgt
    16561 tccaaacatt gttgaatttt tttcatagtt tataaaaaat ccatatagta cgattaataa
    16621 taattgccat aattgtggct ccattatgat ttcacctctc ccaaaatatt ttttagttct
    16681 ttaaaattat aactggaatc ttgctttagg aaaataggaa tttcttccag tgggcattca
    16741 acttcataag ttttcccacc ttcataacta actccactaa atatattttc ccatgtatgt
    16801 ccttttggta aatatacttt tctacttcta tctttataat tcattattgg agctaccaaa
    16861 atttcatctc caagcatata tgtattatcc acctcccaag ttttatcatc ttcaggaaat
    16921 tcataaaata aagttctcat aactggtgtt ccatcttcat gcgcttcttt catcagtttt
    16981 tttaaataag tttttaagct ctctctaatt tttatgaatt ttgtaagaat tacttcaact
    17041 tcttcaccat aagaccatat ttcattagga gctccagtta ccattgagcc tccaccttta
    17101 tttgataatg gtttactatg aggcaatcta tctccatgca ttctcaaaat tggtgaaaaa
    17161 gtagcatatt ggaaccatct taccataagt tctctaaatt ctgggtcttt aggatttcct
    17221 ccatggaacc ctccaatatc agtagtccac catggtatac ctgccagtcc catatttaac
    17281 cctgtattga cttgattatt aaaagcctca aaactagaat caatgtcacc agaccatact
    17341 aaagctccat acttttgagc tcctgcccaa gctcctctta caagagttac aacagactct
    17401 atgtcattat gtaaaccatc ataaatcatt tttaaataat caataggata aatattacca
    17461 caacttaaaa catttccttt tttatatcta tagttatcaa aatcatacac agaatagcca
    17521 ggttcagcta catctaacca ataatagtca atcccaagtt gtttataatt tttatctatt
    17581 ctatcccaaa catattctct tgcattttca tttgtcatat ctacaaagac tgtatttcct
    17641 tgaatttgca ttgtcattct aacaccacga ttaacattga ctaaatagcc attttctaaa
    17701 taatcattgt agttttctgc atcactttga actgtaggcc atacagatac tattggttct
    17761 actgaatact cttctttcaa ttttttaact aattctttag gatttttcca ataatctaag
    17821 tcaaatttat attcaccttg cttaggccaa tgaaaatagt caattgcaat tgatgaaagc
    17881 ttaattcctc ttttagaata ttccttcaca acatctaaaa cttcttctga agttctatat
    17941 cttagtttac tttgccatag tcctaaaagg ttttcaggca tcataggaac agttccagta
    18001 acttgagagt aatttttatt taactctttt gggctttctc cacaagtaat ccaataatct
    18061 ataaaatttg tagaaaacat cttccattct gtcatatttt tagcaaaaga tacttttcca
    18121 attcccggat tattccataa aagtccataa cctagactag aaatataaaa aggaactgat
    18181 atttgagaat ttctttgggc taactccaaa acagtattct ttaaatctaa aaatttatgt
    18241 tgatattgtc ccattccaaa aatcttttca gaaggctcag attcaaatct agtagttact
    18301 tcaaattctc cactatagct tggtcgatat tctcttgatt ttaactttag agtagagtta
    18361 aagtctttgg taatctcaat tgtccctaca tcttctccac catcatcatg ttttacagct
    18421 cttaatcgaa tatattcttt caataatatt tcatttttat cattataaaa agtaattcta
    18481 tctctatggt ctaagacagc ttttatcttt ccatttttaa tgattgcact tccatcttca
    18541 tttttattaa ttgtaatatc atctttattt aacttaggtt tttcgttaag agcatagttt
    18601 tcatcaacaa aattttggtc tacaaacgac ctaatacgta aagagttttt tccccatgct
    18661 tctattcgca atagttcatt gtcaaatctt ttttcaatat agtcttcaga tatttttatc
    18721 acggctacct cctaaatcaa tggtataata ttctatattt gtgtagctta agttaaataa
    18781 tgtcgtttag tatcgttatt tataaattag gttttatata taattcattt gtacattaaa
    18841 ctaacttacc aataatatac tttatttgac tttgtttgtc aatcgctttc cggattttag
    18901 taaaattttc taatatattg ctggaaagtc gttcttaata tcttttattt atacttttaa
    18961 tatctgtgat gatacctata tacatctagt ttttttaaaa cacatacagg aataacattt
    19021 gcctattatg tagataatat cggacttgaa aatatgtata gtgaccttat a
//
LOCUS       DAEMHZ010000068        19000 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000068 DAEMHZ010000000
VERSION     DAEMHZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.068"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(373..750)
                     /locus_tag="KPK72_002248"
     CDS             complement(373..750)
                     /locus_tag="KPK72_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2489460.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(937..1908)
                     /locus_tag="KPK72_002249"
     CDS             complement(937..1908)
                     /locus_tag="KPK72_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2489461.1"
                     /translation="MFLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1942..2139)
                     /locus_tag="KPK72_002250"
     CDS             complement(1942..2139)
                     /locus_tag="KPK72_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG2489462.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(2207..3418)
                     /locus_tag="KPK72_002251"
     CDS             complement(2207..3418)
                     /locus_tag="KPK72_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG2489463.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYELPK
                     QYYKRKDGYIE"
     gene            3699..4301
                     /locus_tag="KPK72_002252"
     CDS             3699..4301
                     /locus_tag="KPK72_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2489464.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            complement(4557..5528)
                     /locus_tag="KPK72_002253"
     CDS             complement(4557..5528)
                     /locus_tag="KPK72_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG2489465.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(5554..7035)
                     /locus_tag="KPK72_002254"
     CDS             complement(5554..7035)
                     /locus_tag="KPK72_002254"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG2489466.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            complement(7057..7203)
                     /locus_tag="KPK72_002255"
                     /pseudo
     CDS             complement(7057..7203)
                     /locus_tag="KPK72_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            complement(7372..8358)
                     /locus_tag="KPK72_002256"
     CDS             complement(7372..8358)
                     /locus_tag="KPK72_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2489467.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(8743..10497)
                     /locus_tag="KPK72_002257"
     CDS             complement(8743..10497)
                     /locus_tag="KPK72_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG2489468.1"
                     /translation="MYIMNKKFKKFSKNLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(10715..11605)
                     /locus_tag="KPK72_002258"
     CDS             complement(10715..11605)
                     /locus_tag="KPK72_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2489469.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            11754..12614
                     /locus_tag="KPK72_002259"
     CDS             11754..12614
                     /locus_tag="KPK72_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2489470.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            13050..13616
                     /locus_tag="KPK72_002260"
     CDS             13050..13616
                     /locus_tag="KPK72_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2489471.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            13656..14237
                     /locus_tag="KPK72_002261"
     CDS             13656..14237
                     /locus_tag="KPK72_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2489472.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(14294..15211)
                     /locus_tag="KPK72_002262"
     CDS             complement(14294..15211)
                     /locus_tag="KPK72_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2489473.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            15341..16972
                     /locus_tag="KPK72_002263"
     CDS             15341..16972
                     /locus_tag="KPK72_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2489474.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            16975..17922
                     /locus_tag="KPK72_002264"
     CDS             16975..17922
                     /locus_tag="KPK72_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG2489475.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(18207..18470)
                     /locus_tag="KPK72_002265"
     CDS             complement(18207..18470)
                     /locus_tag="KPK72_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG2489476.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(18569..18814)
                     /locus_tag="KPK72_002266"
     CDS             complement(18569..18814)
                     /locus_tag="KPK72_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489477.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         6480 a         2853 c         2324 g         7343 t
ORIGIN      
        1 taccaataat aaagtttaaa aatataaaaa tcatttctta cttttagttt acctataata
       61 acaaaagaca ggtttatatt gtaataatta aaaatagtaa aatatatttt gatatggtta
      121 aatagaaata atactttaga ttaatgaata ttgacaagtt ataaaattat tttaaatttt
      181 aaaaatcatt tcttatttaa ataagtggta tcatttaact tgatactccc tattccaatt
      241 attttaggca ttttactaat tagagaacct atttaatatt actttatgaa tagtgagtag
      301 ctgattaaat aataccattt atggttttca gccatagatt aattaaattc ttagagttct
      361 atcctgatat aattactctt cttctaaatt tttaaaatga ctttcaaata aatccaacat
      421 atctttatca acaacttcat catcatgtag tgctgaaatt aaactctgta aagaattgtc
      481 gtgtatattg cttaaaaaat cttttgtctc aacacttaaa tactccttat gtccaacaac
      541 tattgtataa tgagtgtatc tatctatttt ttctgcactt aaaaaatgtt ttttaacaag
      601 tcttgataaa agtgtcaatg tagttgtttg tttccaacca tattttcgtt ccatagcttc
      661 aatagtttct tttgatgtta ctgtatcatt tttttcccaa ataaacttca taacttttaa
      721 ttctgcttgt ggtatttttg aaattgtcat aaccttacgc ccctctcaaa cttttattaa
      781 attaatacta tcgcaatata gtttttaagt caaagatttt gtcgataata atattttcca
      841 atttttacca tagggttatt ttttatataa ttctatgata aattacataa aaaatattta
      901 ttttacatca taaaattaga aatgtaaata gtatgattat ttagtcttat ctttaataaa
      961 caatccagta aatttataca tccaaagtgc attcataaac aacaataaac ttgttgaaaa
     1021 aaatactgtt ctaataccta aatgtgctga tatttgtccc cctaaaaatg ctcctgatga
     1081 acagcctata tactgggcag atgcattata tccaaatatt tttcccacat attcttctgg
     1141 tgcaattttt ttaagtaagc tgttaacaga aggattaagc cctgcaactg acaaccctaa
     1201 taaaaatctc aacaacatca gttgccatgc agtttttaca aaagcttgtg gtataaatat
     1261 aatcccagcc cataaaagtg aaattaacaa tactttttga ggacctattt tatccgacat
     1321 ttttccaagt ttttgagctg ctaataaatt tgctaatcct gatgcagaaa atgtcaatcc
     1381 agctattaaa gctacatttg atgcaaaatt tgtaagttgt gatatatata tggttacaat
     1441 tggttctata gagtataaag ctagttgagt aatgaatgtg gttacaaaaa tagatgcaat
     1501 taaaaattta tttggcaata tatttaattg ttctttaggt atagaaattg attcttttat
     1561 ttctttaggt ttaaaattat ctactacaaa taaaatagag atgataaatg atacaaacaa
     1621 caatcctcct gttataataa atgtactttt aagacctaag attgattcta tatatcctcc
     1681 tactgttgga ccaattaatg agccagtagt tgatgcagtt gcaagagtac ccaaagccca
     1741 tcctgaatga tttttatctg tctgtgttgc aataagagta gtacatgcag tagcataacc
     1801 cgtaaaaact ccttgaagta ttcttaatcc taataactga taaacatttt gaacaaacgc
     1861 catggtaaat ataactattc ccatcccaat acttgcacgt aaaaacattg attttcttcc
     1921 atatttatca gcagctttcc ctcaaattgg cgaaaagaat gctgctacta caaaggtaca
     1981 tccaaaaatt atacctgaat actgttgtat taaactaaca tcagttaccc ccaactgttt
     2041 aatatataat ggaagtatag gtgctatttg gctcatccct atagaactaa aaaacatacc
     2101 tatccaacat acaaataaat ttcttttcca caattccata tagaattaca ccccctgcat
     2161 tttaaatttt ttcaataata ctaagtttat taatttatgc tattgtttac tcaatatatc
     2221 catcttttct tttataatat tgtttaggaa gttcataact cgaaaataat agtggtgata
     2281 aagaatagga gtaacttcca gcattactaa ttgaaatcaa atctcctatg tttgcttttt
     2341 tcaatttaac attatttgca attacatcta atgatgtaca gagattccca acaacattta
     2401 catactcttc ttcttttaaa ttatttaata ctgaaagaga aaaggcatga tttgatgtat
     2461 ataatggctc ttgaccttct aattctactc cacaagtaat attttcaatt aaattagaaa
     2521 taataggtct cataaatcca ttaagtccat tttttacaat aagatatttt ttccctcttg
     2581 atactttaat atctacaatt ttaacataat aattacctga tttacaaact atgaaacgtc
     2641 ctgattcaat aagaagtctt gattttagtg tgtttgaatt agtaaaagca atcttttgag
     2701 ttaattttga aagctcttta agattaagtg gattttctct aagattatca taagctactc
     2761 caagtccact accaaaattt ataaattcaa tattaatata cttactttta tttaaattta
     2821 aagctataaa aaaacatttt tcataataat gtcctaactt ttcaaaatcc aatacctgag
     2881 atttaatatg tatatgaata ccaactacag atacataatc acacatactt aacatcttaa
     2941 aaacactgct catatcttct atatctatac caaatttgct tgatttagca ctatcatcat
     3001 ccatagtaaa atcaggattt attcttattc ctattttaac tttctgatta tgctttttag
     3061 atatcttatt tataagttct atttctgtaa tactatctgc aataattatg catttatcta
     3121 aaacagtttc tatatcaatc tctgttttgc ctggtgatga ataataaata tcatctttag
     3181 aaactccaca ttttgatgaa atcataactt cattagttga agaagcatct gctccaaaac
     3241 cttgctctgc aatgcttttt acaattttgt tatatggatt tgtttttata gaatataaaa
     3301 aatcaaaacc taaaatatta tttttcaatt ccttacattt ttcaattata ttagtctcat
     3361 catatatata acatggtgca ttttcttcta taaatttata gtcttcattt gagtacatat
     3421 tttttacctc caagatttta aatataatca taaattaagc aattataatc ctataagata
     3481 attttacaat aaattaccat agttaactac acacatatat gtaaaaacat acctaaaata
     3541 tttttaatta tagatacatt ccaactttta ttgataaaac aatttgtttc aattttatta
     3601 caaatataca aagtgtattg ttgttaacgt attctcatag tatcctaaaa ctatattaag
     3661 cactaaaaac tattttaaaa tactgtggag gtatcaatat ggaagtatgc attcttatgg
     3721 gaagtcctaa gaaaaaaggt aatactgctg ctattttaaa accttttatt gaggaattag
     3781 aaaattataa ttcaaatatt gaagtaatat ggttatatga ttgtaaaata gagccatgta
     3841 ttgcttgtaa aaaatgtcag gataacttat ttgattttgg atgttattgt aaagatgatg
     3901 ttcagaaaat tttcaagaaa attcttatgt gtgatttaat tgtttttgct actccaattt
     3961 attcttggta ctgcacttct ccaatgaaag cactacttga cagattggtg tatggaatga
     4021 ataaatacta tggtaatgag aaaggccccg ctctatgggc aggaaaacca gtagcactta
     4081 ttacaacatg tggatataga cctgaaaagg gtgctgatat ttgggaaaat ggtatgaagc
     4141 gctactgtaa gcattcacag ttaaaatata taggaatgct tgttgaacgt gatttaggta
     4201 aacctaattt tatgaataaa gaaaaagaag aacattctcg tcaatttgca aaacaaattt
     4261 attatatttt agataattca attgaagtag ataactgcta aagacataat actccaagta
     4321 aagtattgat aatataaata aatcttacat aatttgctta tttaaaataa gatatatatt
     4381 cttctaaaaa gtaacaataa ttaaaatatt ttttatcatt tcaacaatga taaagaggct
     4441 tatctctatt ttgagacagc ctctttattg ctttttatat gttaattatt ttcatagtaa
     4501 acatccttaa aaactcttta catgttactt ccttcaaaac ctattactca ttcctactat
     4561 aaatttttta taaattcagt tatttcttcc tttgttgcat atgaacttat cgcaccttta
     4621 atagttgtac tacgagctgc ataataatta gaaaattcta aatactcatt taacgtttct
     4681 tgtttcattt cttttatatc attcaatgtt atttgtttat acaataaggt gtataaaaat
     4741 gaacctatat aagaatctcc tgctccggtt gtatctattg catttacttt aaccccttta
     4801 cacatacctt ttactttttt agtgtatgca tctactccat cttttccttt tgtatataat
     4861 actagttcaa cattccccac aaagagttta tgtaatgatt tttctatatc attttctcct
     4921 gttataaatt ctaattcttc atcagatatc ttaacaatat gagcaaaagg taaaaattct
     4981 gatattgcct ttttacaaga ttgctcacta tcccataaag gtaatcttat attagggtca
     5041 aagcttatta tactattact ttcaagtgcg tattctattg cttttttatg tgctaatttc
     5101 attggactat ctattaaatc tactgaacaa aaatgtagga tatgacaatt attaaaccac
     5161 tcttttttaa cttcatcagc ttctaaaagc atatctgcac ttggatttct ataaaatgag
     5221 aaatcacgat taccatcttc ttttaatgat acaaaagcaa gtcctgtatt tgctttactt
     5281 gttctcaata cataatcagt atttacattt acttcattta atacatctat tatataatca
     5341 ccaaatgcat cttttcctaa cttagatata aaactcgatt ttcctccaag ctttgctaca
     5401 acagcactaa catttgctgg agctcctcct gctactcttc taaattctga tacatttttt
     5461 aatttaccac cattttgatt cggtataaag tctattaaag cttctcctat gctaattact
     5521 tttttcatct tttctacccc ttataatgtg tttttataat tcaatacctt gctttaattt
     5581 aaatttagta tactcatact ctactatttt atcactataa acccttattt gattagaatt
     5641 ttctttagca aatattctac tagtaaatac tacttctcca tcatttataa aaatttctaa
     5701 aatacttcta tccgctagta ctttgatatt agtattttca tcaatattta tatattcttt
     5761 tctatttgtt ccatattcaa caccgaaaac ttttttaaag ttgcttctgt ctaaagttac
     5821 aatgtttcct aatttgttaa attctaattt tacaccttca tcttgtgatg aaaataattc
     5881 cagcccaaat ttattgctgt caatattttt tatattaata ttaagctcat atacattttc
     5941 atcattttct ataatattaa tattattctt tatagtattt tgcccacttg tttcatcata
     6001 tcttaattta attaattcat ctactggcat ttgcaatagc ttattatttt ttatatttaa
     6061 ttttcttgga acagttagac aatgtgccca tttgttttta tctgtagggt ataaaacttc
     6121 tcccattcca gcccatgcaa atagtattat ttgtgagtcc tttataaatg tttgtggagc
     6181 atagaaatca aatccttttt ccagttcttt cattgtatct atttcaaatg ttaaattatc
     6241 taaatccata ttaccaatag catatactac attatatata ttttgataat caaatccttt
     6301 aggttctact ccttgtggtg aaaatattaa tatatctttt tcatctatat taatataatc
     6361 tggacattcc cacatgtaac caaaatcttc atctatattt tttacattta attctccttt
     6421 aaaattccaa tctatagagt ttttagattc atatactatt ataactcctt ttttatcact
     6481 tgtttgtgct ccaagtaaca taaagtattt atcctttctt ttaaatactt ttgggtctct
     6541 tacatgtcct gtatatcctt ttggtatttc acttactatt ggattatttt catatttagt
     6601 tattttacca tccttgttca ttatagctaa gttttgatat gcatatctat cttctgcact
     6661 atattttata ttccctgtat aatacatatg caattttcca tctacctcta tcgcattacc
     6721 tgaatatgct ccatgagatt cataactttc gatcggtatt aatgctacat cttctctatt
     6781 ccagtttaca aaatcatcag aacttacata agcccaatgc ttcattccat gagttgcatc
     6841 atatggatac cattgataaa atacatgata cttaccatta taatatgcta atccatttgg
     6901 gtcattaagt aggccatgtt gtggatgtat atgaaatagt ggtttatatt tatcatttaa
     6961 agatatattt cttaatttat ttaattcttc ttttgtcgat tctaatatgg ttctatattt
     7021 aggctctttg tacatatttt tattcctttt cttttataat tttatatttg ctttattatt
     7081 actatctaaa acaattctta atcttgttac acaatgtgct acacctttta tattttattt
     7141 atttcctatg tactctaata attactttga tactttgtta caatcagtta tttttgcact
     7201 cattttgcta ttctatcggt ttaattaaac cggtttcgaa accggtttaa ttaaagatta
     7261 gcataaattt atttcagatg caatatttct ttcaaaaaag ttttcctaat ttttagaatt
     7321 atagtttttt tagtaaaaaa atacacatta ctaatatatg taatgtgtat cttaattttt
     7381 atttattgta ctttcacctt ctattaattc aaatttaatc tcctttaata gtggttcttt
     7441 tctttcttgt attaagttta ctatcgtatt tgctgctact ttacctgcat ttttattttc
     7501 aaactttatt gttgtaagtt tagggcttat aatagacaat acatcatatc ccccaaatcc
     7561 agctacagat atatcttgtg gtatattttt acctctctta ttgatttctt tcattgttgc
     7621 tattgctata ttatcagttg cacatattat tatatctgga ttttcacttt ctagcatttc
     7681 tccacttctt tgtatagacg tttcttggtc aaagtcagta taaaatactt ttgcatcata
     7741 ccctttatct tttaacccat taagtactcc attttttcta tttaaaccaa ctgatatatc
     7801 actttcatca acacctaaat atacaatgtt tttgtaacct ttattagcaa tatattgacc
     7861 cattttatat cctgctccat aatcatcatt cactatgcta ttgataccat caactttttg
     7921 tccaactaca actattggta tatctaactt ttcaatttca ttcttatgtt ctttagttat
     7981 ttcggtacca attagtatta ttccatcaac ttttagacta gctaactttg atatactatc
     8041 tatttctgac cttatttttc tattagtatt aataattagt gatgtatatt ttaattcttt
     8101 gagttcttca tctattgcca tcataatttt agattttaca aaagaatcta aacacggtgt
     8161 tataatacca ataaagtatg tttttttagc tctcaaactt tgagcaaatg cattcggctc
     8221 atatccatat tcatctatta ttgtctttat tttctcttta gtagaatcac tgatatcttt
     8281 attatttaga tatcttgata cagtactttt agatactcct gccaagttag caatatcatt
     8341 tattgttatt tttttcatag gttatcacct tctacatttg atatatttta ttacattata
     8401 ttatatattt aaaataaaat atagttattt gtccagtcaa tactttttat atactacttg
     8461 tactatccat atccacatcc aaagaattga gtctttaaca ttaaattaat tgtaattttc
     8521 attttttgtt gttatattta atttcaattt tatctactat gaattgataa atactttaat
     8581 ttatttataa tataaaaata aggctatgat ttttacaacc atagccttat cgataaatac
     8641 ttaattatca cttaaaaaat aattttttat ttaaagaata tatataaata ttttaaaatt
     8701 ctataatttt taaaactata taaactttat tttcatatgt ttttattctc caattgatat
     8761 attatttatg ccattatttt taacttcaat aattggttgt ccatttccta aaatataatc
     8821 tttagaccaa ccttctggca atttatttat cccatttcca attaacttca agtgtaaatt
     8881 tgatggttct tttttcagat ttaatttaaa tattccatca aaggtatctt tattatcgct
     8941 tccaaataaa cttatattgc catttatatt tgtagctgtt acatttacaa ttccattgtt
     9001 tgctctaaat tttatttcag atgatatagt ttcagctgtt acacctgaaa tcccactatt
     9061 agtatttata ttgaaaggat ttgttacatt gttatcaaca atgttagcaa ttccgtcatt
     9121 gacatcaaca gtagctcctc ctttaatatt ataaagttct actgaaccac tttcaacatc
     9181 tactttaaaa ttagaataca ttttatcggg aactccaatt tttatcacat ttaccatcct
     9241 atctttattt gcatttatca tattgtcgcc attcatatct tgcaaatcac tatctttctt
     9301 atctattttt ttcttagcac ttacaactaa ctttccattt tttatattat tagaagaatt
     9361 ttctttatat acagaaaaag tacaattttt ttcattagtt acacttacgt aatcaaactc
     9421 aaatttattg gtattcgtct tttcaacaac cacacctaaa ttatctgatt ctataagtat
     9481 ttcagaaact tcatttcctt caactgaagt taagtgtttt aaaatatttg gagtcatatc
     9541 catattatta tatttaatca tatctaaaca ctgtttatat atattggcat caatacctcc
     9601 aacttttgtt atacttttag ctccttttaa aataccttta ttacttccat cagcaactaa
     9661 aacgattggt ttttctttgg ctaaaggaga taatgttagt gcatctacta attgatatcc
     9721 atttgttata taaaaatctg tagctccatt ataaaacttt tccattacct ttttattggt
     9781 ctcaaatcta tcatttcctc cgattctagt tgctttagtt tcatttacta aatcctcatt
     9841 tatcgcagat ttgccaccta tagcgtaaac atttaaacct tcagtcttaa atggaactga
     9901 tttcccatct gtaagtatta tagctccttt attttttgca actacaggtg ctatactcat
     9961 agcatcagcc tctcctacaa atccatttgt aagcattacc tcttttacac tacttacttc
    10021 atttattttt ttagctacat tataactagt ttcaattcta ttgttaccat ttattctttc
    10081 cacttgaata ccttttcttt ttatatcttt ttctactgaa ttttcaatag tattataacc
    10141 accaattata tatacttttt ttacaacatt taaactctta ttatctagtc tatttaaagt
    10201 agcttttggt atttcacttt ttttagtcaa aagtattggt gcgttaatag ctccagctaa
    10261 tccacttgat gaaagcccat cagataaatt tttatctcca ttaactaata ttacagtatc
    10321 ataagtttgt ctatccgcta taagtcctgc tgtttcatat ctatcaattc ctattattga
    10381 atcttcttta atctgtgcat ttgcagttaa attattaaaa agaaacatag tcaaaactaa
    10441 aattaatata cttaggtttt tactgaattt tttgaatttt ttattcataa tatacactat
    10501 tcctcctgta aattaaggtc tttaatttgc ttataaatta tccccttatt tacaaataat
    10561 aacataatat tatagtaacg tatatattac cttaaaatta tcagtaattt tatataaatc
    10621 atcttagctt atttatataa atttttttat attacttttc aaataacagt catactaaaa
    10681 caatttcatc atataatttg aaaattatta ctatttacac agcttttatc atcatcttac
    10741 caccatcaaa tatatattca accatattat tctcatataa ataagttata taagaacgta
    10801 ttgtacttcc aaccaacaca tattgattag catcaagctt gacattgtac aaatcacaca
    10861 ttttttcaat aacttcatca atcataactg gctcataaca aaccttttta atattatcta
    10921 ttatttcttc catcttcttc ttattaatat taatcaaact ttttatatct gtagttttga
    10981 cagagtgact tggaacaaag aaatcagcct ctaacattct caatttttct agtgtcttaa
    11041 actgtgaatc tatatccagt aggaagaaaa aatgatattt agtaattata ttctctggtg
    11101 taagactatc tgcaacaaac aaaacattat ctggagttct aacaccaatc atttcaaaat
    11161 aatgacctgg aagagaaatc gcttcaagtt ctgtatctac aattttgcca tttgaaggta
    11221 taatataatc aacttcactt tcctttgcta ataaaaattt attcttcagt gctttacttg
    11281 gatagccacc atataaaaat gctgattcta atattggatt ttctgtaaaa tatccttcaa
    11341 ttttagtcgt agcaatcttg cattttgttt gattcttcaa atatgcattt cctcctatat
    11401 ggtctgcatt tgaatgagtg tttattatta atttaggatt taaattatgt tcttctaata
    11461 atcttaaaac ttgtctagct gtatccttat tatttccact gtcaattaaa attgcatcct
    11521 taccatctac gtatacacct actattgtag ggagtggaat ataatatgta tttcctttta
    11581 tatgttctag agttaattta gccattttat aacctccctt taaatttact tttaattata
    11641 gcattaattt tctgaaaatt ccaatcaaat tgtttttata atatattttt atttctttta
    11701 ttaaaaattg gaaaaactat ataatatata ttataatcta tatataagga gtgatgttat
    11761 tgaaaaaaga tagaataact ttttcagaaa ttttaatatt aattgtaggc tgtatattaa
    11821 tggccatttc tcttaactta ttttttaatc cacatgcaat cgctgctggt ggtataacag
    11881 gacttggagt tgttttaaat tcattattcg gtgtagaact ttggattgtc aatttacttt
    11941 taaatgttcc attattcata tttgcttaca aaatactttc aaaaaaagat tgttttaaaa
    12001 cggttttagg aataattttt cttaccatag cacttaagct tactgcaaat atggctacat
    12061 tagatataac aaatgatatg tatcttgcaa ttatctctgg ttctatttta atgggagttg
    12121 gtcaaggact tatttttaga ataaatggct ctacaggtgg aactgattta atggcactcc
    12181 tactaaataa atattttcct acatttagta tacctgtttt aatgggtatt gtagactgcg
    12241 ttgtagttgt tttatcaggt atagtaaata gacaggttga aattgcactt tattcaactg
    12301 tggctttata cattttagta aaagttagtg acttgcttat tgaaggattt aattattcca
    12361 aatcattcac tattatatct gacctttcaa aagatataag taaaaaaata atggaagatt
    12421 tagatagagg tgcaactatt ctaaaaggag aaggtgcata tactggagaa aataaaaatg
    12481 tacttttagt agtagttgaa aagaaagaag ttgtcgaatt aaaaaaatta gtaaaaaatg
    12541 tcgaccctaa tgcttttatt ataattacag acatacatga agctttaggt aatggattta
    12601 aaaaaataga ataactaaca aataaatagc aagtataatt aacatataca aattcaactt
    12661 tttatatgtt atattatact tgctatttat atattataat tgttgttttt aagataaaaa
    12721 ttaaatgtat caattattgt tttatacaag tatttccaaa tccgttctca tttagatagt
    12781 atgcttagaa attttagaat tattgagtct aactttatta cttacttcac tttataacaa
    12841 tcaaatacaa atttaaatat tataaaacta caaaataatt taaagcatat aatgtcaata
    12901 tgtttaaatt ataaaatatt catgtagtta tataataaat aatttataaa tatagccaac
    12961 ttagcctaaa tacgtatata atatagctaa tatggttctt atacttgata ccatcaataa
    13021 aactaaaaat ataaaaagga gaacttaaaa tgagtgttag tttacaaaaa ggacaaagag
    13081 ttagcttagt aaaagataat aatcctgtaa aaaatctagt agtaggctta ggatgggata
    13141 tgaataaatt gggtaaaaaa aattatgacc tagatgcatt tgcaatagca ttgactaacc
    13201 aagataaaat gaatgttaga ggtgacttgg tttattttgg aaatcttaaa cacccttcta
    13261 aagctattat acacactgga gataacttaa ctggaaaagg tgaaggtgat gatgaacaaa
    13321 taattgttaa tttagaagac atacctgaat atgtacataa aatagttttt ggagtttcta
    13381 tatacaaagc caaaaaaaga gaccaggatt ttggtcaaat gaacaactct tttataagat
    13441 taattgattc aaattccaag caagaattat ttaaatataa tcttcaagaa gacttttctg
    13501 gtaaagtaac agttttggca ggagaaattt atcgtagaaa tgaagaatgg aaatttaatg
    13561 ctttaggaat tggtcaaaat gaagagcttc gtgaattaat taatacatat aagtaataat
    13621 tacaataaaa aatttataat taggagttgg tttaaatggg tataacttta gcaaaaggac
    13681 aaaaagtaag tttaacaaaa agtaaccctg gtctaaaaaa agtaatagta ggactaggat
    13741 gggatattaa taaatatgat ggtggctttg atttcgattt agatgcctct gcatttttaa
    13801 ctggaactga tggaaaagta acgaatgatg gagactttat attctataat aatttaaaac
    13861 acgcttctgg agctgttgaa tatatgggtg ataatagaac aggtgttggt gatggtgatg
    13921 atgaacaaat aaatgtagac ttatctaaaa tacctcaaaa tattgctaaa atatcattta
    13981 gtgttactat aaatgaagca attacaagaa gacaaaattt cggacaagtt gaaaattcat
    14041 atataagaat atataatgaa gaaactaatg aggaacttat taaatatgaa ttgggtgaag
    14101 attttagcat agaaacagca atagttgtgg cagaactata tagacataat ggagagtgga
    14161 aatttaatgc tttaggttct ggatttgagg atggacttgc aggattgtgt aaaaattttg
    14221 gcgttaatat aggctaatat gtctaaataa aaataagagt atattgatat actcttattt
    14281 ttatttagat atattataaa ctgtttacac ttctactctt ctttctcgct tgttttttcc
    14341 catattctac tgaaatatca aaaaatgctg aaatattttc tgtaaaaaac ttgcttctat
    14401 gatatgcaat atcaaatgtc ctatgtaaat taataccttc aatatcacaa gcccacaaaa
    14461 gatgcttttt taaataatcc tcaataagta attcggatat aactgcaatg ccaaaattat
    14521 ctacaactgc tcttaatata gcttcagagt tataacatga ccatctaata ttcattggga
    14581 tttttaattc ttttaattgc ttttctaatt gtgctcttgt accacttcct aattcacgca
    14641 taattaatgg ttgatttgaa agttctgaaa cttttataga gtctctttta taaaactcat
    14701 gcttgtgtga acatataagt acaagattat cattaataat gctttttgta acaatatctg
    14761 ggtgcttaat ttttccttct acaatcgcaa tatctaattc actattaagt aatttttttg
    14821 caatcagtct tgtatcttct acaaatactt caggttctat aagaggattt accttgtata
    14881 attctaacat tatcgggctt attacacatg taccaacagt tactgttgcc ccaactctta
    14941 tacggatatt ttttgcacaa tgcttcatac aatcattcat ttcttcattt acagctaaaa
    15001 gatgcctccc atatccaaga agttttttcc ctgcctcagt taaatataat ttttttgaaa
    15061 ttctatcaaa tagcttaaca ccatactctt tttctatttc tgaaatagct tggctaactg
    15121 atgactgggt aatatacata tttcttgcta cttcactcat tttaccacat tcagcaacct
    15181 taacaaagat ttcaagacta cgaattgtca tattatcaca accttagcaa acattagtaa
    15241 atgcttatga ttttattaat attttagtat tttacaaata tattgtcaag aagtataatt
    15301 ttattataga gttataaagt agattggagt gtgatatatt gtgaaagtat taattatagg
    15361 tggagttgct gctggaacaa aaactgctgc aaaaataaaa cgtgaaatgg gtgataattg
    15421 ctctgttaca atactaaata aaggtgatga tatttcttat gcaggttgtg gtcttccata
    15481 ttacgttggt aagataattg aagataaatc atctttaatc gtaaatacac ctgaaagttt
    15541 ttctaaacta acaggagcag aagttcgttg tggagttgaa gttacgtcta ttgatagaaa
    15601 tgcaaaacaa gtaactttga aaacgctttc aacaggagaa aaagaaaatc taagctatga
    15661 caaacttgta attgctacag gggcagaccc aataaaacca ccaatacctg gaatagattt
    15721 acctggagta ttctttatgc gtactccaaa tgatgctata aaactaagag attctattga
    15781 aaacggtatt aagagagctg ttgttattgg tggaggtttc attggtcttg aaattgctga
    15841 aaatctttct gcaatgggta tacgtgtaag tgttgttgat atggctgaac atgttatgcc
    15901 tggatttgat actgattttg ctgaatacgt tgaaaatcat atggctgata aaggcataat
    15961 gatttttaca ggtgaccagg ttgtaggtat tgaaggtgaa aataaagttg aaaaacttcg
    16021 tactaaaaat agagctatta aaacagattt agtggttatg tcagttggaa tccgtcctaa
    16081 cacaggtttt ctaaaagaca caggtttaga atttgcccct aataatacaa ttattgcaga
    16141 tgaatatatg cttacaaatg ataaagatat atacgtagtt ggagactgtg cttttgtaaa
    16201 aaattcttta acaaataaac ctacatggtc acctatgggg tcatctgcaa atcatgaggg
    16261 tagaatttgc gcacaaaata tatctggaaa atctaaaact tacaatggtg ttcttggaac
    16321 aacagtagta aaattacctg agctcaatgc aggaaaaaca ggtctaagta aagaaatagc
    16381 tgaaaaagaa ggttataatg tttgctctgt aaccatagct accgatgata aagctcatta
    16441 ttatccaggt gcatctaatt ttattataag aatggttgct gaaaaaaata ctagaaaatt
    16501 attaggtgtt caagttatgg gacctggagc ggttgataaa atagtagata tcgctgctac
    16561 tgcaataact ttaggcgctg acctttattc attagaaagt atggaccttg catatgcacc
    16621 acctttttca acagctattc acccttttgt agtggcagta aatgttttac aaaataaact
    16681 taatggagaa cttgatagtg ttttacttga cgaaattgag gattttgaca gttggactaa
    16741 acttgatgtt tcaaaagctc cttctattcc tagtttacgt tatattcctg ttggtgaaat
    16801 taatggagaa attgaaggtc ttgctaagga tgaaaaaatt cttcttatat gtgctaaagg
    16861 taaaaatagc tatatggcgc aaaatcgctt aagacgcttt ggctatacaa atacgaaagt
    16921 tcttgaaggt ggaatactat tttatccaaa tcttaaggta atggaggagt aaatatgagt
    16981 aatgttacag cagaacaaaa aaaggaagta aaaggtagag gttttctacc aagcagggat
    17041 ggagaacatt ttgcagcacg tataataact gtaaatggtg taataaatgc atcacaagca
    17101 aaaaaaattg cagaagctgc tgaaaaattc ggaaacggtc agttagcatt tactactaga
    17161 ctaacagtag aattacctgg tgtaaaattt gaagatattg aagctctaag tgaatttatt
    17221 gctagcgaaa atctaataac aggtggaact ggatcaaggg ttcgtcctgt tgtggcttgt
    17281 aaaggtactg tttgtgttca tggtctagct gatacacagg cacttgctgc tgaaatacac
    17341 gaagaatttt acaaaaattg gtatgatgta aaactacctc ataagttcaa aattggtgtt
    17401 ggtggatgcc ctaataactg tattaaacct gacctaaacg atcttggaat tgttggtcaa
    17461 cgtgtacctg actatgattc agagctttgt gttggttgta aaaaatgtgc tgttgtagag
    17521 gtctgtcctg ttaaagcagc taaattaaca gataaaggta aacttgaaat agacagtaat
    17581 ctatgtaata actgtggtaa gtgtatagaa agttgtaatt ttgatagtat tgaagaaaaa
    17641 gaatctggtt ataaagtcta tataggaggg aaatggggaa aatcagttcg tcctggaaca
    17701 cagattgata gattattttc aaaagaggaa cttatgactt taattgaaaa agcaattctt
    17761 ttatttagag aacagggtaa cacaggtgaa cgttttggaa taactatcga tagaattggt
    17821 gctgataaat ttatagaaat gcttttatca gatgaagttt tggaaagaaa agaagagata
    17881 ctagaggctc ttttacacct tacaggtgga gctgtatgct aacaataaat attttaattg
    17941 tacagataac ctatggaaaa aattttagtt aagaataatt aaaattctat atatgatata
    18001 tgataaatga ggctatctca aaacacagat acgcctcttt ttcattgttg aaatgataaa
    18061 gtatatttga gtttatacaa aaaagagtgt ctcgtgaatt ataaaattca ttttgaggca
    18121 cccacattat tttataataa ttgcttatta attttatttt aactatataa aaagtacaaa
    18181 aagtaagaat atccaatatt ctaaaattaa ttttgaattg tattgattga aaagtttact
    18241 attccactat ttccatcttg atttactact agataccatg ttccagtatt aggaactctc
    18301 attctacatg gatttttctt tgcaaatcca ccatagtaat cataatttct ttgattttta
    18361 aatttcacat aatttatatg gtctaacaat attacattgg cttgtttctc taaatttacc
    18421 tctacaatat cccctttttt taagttcatc ttactataac tatattccat tgaattactc
    18481 ctccagtatc gaataatctt tgttatttac tcatctcaaa tatgtaaaaa ttcatttaaa
    18541 atattacaaa tactatataa ttagactatt atccagtttt aaccttattt actacaatcc
    18601 aaaggttatt aaaaatatta ttggcaacaa cttctccatc ccatacaact ctttcttctt
    18661 tattttctaa taaagcatag tacccagagt cagaactttc atctttatag actgtaacat
    18721 aaaactcttc atcactatat tcaggtatat ttttaaagtc ttcagatatt ttacatgtta
    18781 atatataccc ttctaattct tcatctataa acaaagcaat tcctccaatc tctatatttt
    18841 tactaattat accatattat gtcataaact ttttatttca ttacaaattt ctattttttc
    18901 tttactttat aaataaatta tataaatttt cactttttaa ctttatatta tactaattaa
    18961 ttcttttgtt ttcttatata tttctttata atttataaat
//
LOCUS       DAEMHZ010000069        18752 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000069 DAEMHZ010000000
VERSION     DAEMHZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.069"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            716..1468
                     /locus_tag="KPK72_002267"
     CDS             716..1468
                     /locus_tag="KPK72_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489478.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1458..3569
                     /locus_tag="KPK72_002268"
     CDS             1458..3569
                     /locus_tag="KPK72_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489479.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            3953..4663
                     /locus_tag="KPK72_002269"
     CDS             3953..4663
                     /locus_tag="KPK72_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489480.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYKIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
     gene            5165..6502
                     /locus_tag="KPK72_002270"
     CDS             5165..6502
                     /locus_tag="KPK72_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2489481.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILIFSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(6814..7383)
                     /locus_tag="KPK72_002271"
     CDS             complement(6814..7383)
                     /locus_tag="KPK72_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2489482.1"
                     /translation="MRKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(7735..8748)
                     /locus_tag="KPK72_002272"
     CDS             complement(7735..8748)
                     /locus_tag="KPK72_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2489483.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQVNVY
                     IDN"
     gene            complement(9363..10265)
                     /locus_tag="KPK72_002273"
     CDS             complement(9363..10265)
                     /locus_tag="KPK72_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2489484.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            complement(10392..10511)
                     /locus_tag="KPK72_002274"
                     /pseudo
     CDS             complement(10392..10511)
                     /locus_tag="KPK72_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10674..10877
                     /locus_tag="KPK72_002275"
     CDS             10674..10877
                     /locus_tag="KPK72_002275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489485.1"
                     /translation="MQAQTISYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLP
                     ADCPFLKYLGFNLIPSNQFFLLS"
     gene            complement(11058..11720)
                     /locus_tag="KPK72_002276"
                     /pseudo
     CDS             complement(11058..11720)
                     /locus_tag="KPK72_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(11880..12416)
                     /locus_tag="KPK72_002277"
     CDS             complement(11880..12416)
                     /locus_tag="KPK72_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2489486.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(12489..12902)
                     /locus_tag="KPK72_002278"
     CDS             complement(12489..12902)
                     /locus_tag="KPK72_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2489487.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            13368..14132
                     /locus_tag="KPK72_002279"
     CDS             13368..14132
                     /locus_tag="KPK72_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG2489488.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTVNGMQGNINYMQGTI
                     SNMQGSINGIEGTISDMQSNINGIEDTISDMQSNINGIEGTISDMQSNINGIEGTISS
                     MQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINSMQDSITDMQ
                     VEIKEVNSRLDNMEPDIKDLKTGQVETSSKLDTIQSDVTEIKSDVKINRNTLDTVIKH
                     TSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(14740..14919)
                     /locus_tag="KPK72_002280"
     CDS             complement(14740..14919)
                     /locus_tag="KPK72_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2489489.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(15145..15678)
                     /locus_tag="KPK72_002281"
     CDS             complement(15145..15678)
                     /locus_tag="KPK72_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2489490.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            15995..16345
                     /locus_tag="KPK72_002282"
     CDS             15995..16345
                     /locus_tag="KPK72_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489491.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            complement(17426..17998)
                     /locus_tag="KPK72_002283"
     CDS             complement(17426..17998)
                     /locus_tag="KPK72_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2489492.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHHF"
     gene            complement(18060..18323)
                     /locus_tag="KPK72_002284"
     CDS             complement(18060..18323)
                     /locus_tag="KPK72_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2489493.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(18323..18544)
                     /locus_tag="KPK72_002285"
     CDS             complement(18323..18544)
                     /locus_tag="KPK72_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2489494.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         6757 a         2978 c         2205 g         6812 t
ORIGIN      
        1 ttacaacatt tctattatat tacaatctct tatttattac agttctttat tatactacaa
       61 acctttatta ttacaacatt ttattatttt aacgtcttta ctataatata gtaatatttt
      121 aatatctcaa aatcatatca atcacttttc agcatagtct aaaatacttc cactttctaa
      181 aattatctat catacctata ctaataatac tttttaacct ttttattata catttcaatt
      241 gtttttaacg acctatttat ttccaaaact ccaatatatt ttctcaatta cattgtgtta
      301 ataaccccta aatcctttca aattatttac aatacattcc attaaataat cttactgctc
      361 tataattctt tttttctttt taaccctact atacattatt tacatcattt ttttcaatac
      421 aacattcgtt attctatagc ctttttactt ttactatata ccttttttct aactacacct
      481 tcaatgttcc acatcatatt actttagcac aacaattaat acctaaaaat aacactctaa
      541 atcctccaat aatataatca atttaactat aataataacc tctcacacta tccaaacaat
      601 atattttcaa acattacaaa aaaacatcca actattacaa tattgctcat atccctcctc
      661 ccctttcaaa tgctatcatt gacttacaag ataaatgaaa gtgaggatga tatatatgct
      721 taaagtaaac caattatgta aatcatataa aacaggcaat aaaacatatc ctgtattaaa
      781 aaatatttct tttgaaatca acaaaggaga gtttgtcgca atcatgggtt catcaggaag
      841 tggaaaaact actcttttaa attgtatttc atgttttatc ccaattgata atggggaaat
      901 aattcttgga aataataata taagaaattt aaaagaaaag gaactttcag aagttagaaa
      961 ccaacaacta ggatttgtat tccaagattt catgctattt gatggtctta gtgtctttga
     1021 aaatatatgt gttccacaaa ttatcaaagg tagtcaagtc ttacaaatgg aaaaccgtgc
     1081 aattaatcta tgcgagctat ttggtattga acatatcaga aaaaaatacc ctgccgaaat
     1141 atctggtgga gaaaaacagc gtacagcagt agcaagagca ctcatgaaca acccagattt
     1201 aatattagca gatgaaccaa ctggtaacct tgatagtaag tcttgtcgta cagtcattga
     1261 atcattttta aaagcaaagc aagaattaga tgctactata ctcatggtta cacacgatag
     1321 ctttgcagca agcttctgcg accgagttat tgtgcttaaa gatggaataa tttataaaga
     1381 agttgtaaaa tcaaattcaa gacataaatt cctagatgaa ttactagaag ttttaaaaga
     1441 aatgggaggc gctcaagatg acaattaata aattatacag caaacttcga gcctataatt
     1501 ggaagaacta tacaacttta ttcttttgta taatactttc tgtggtatta gtaacttcat
     1561 atgctctaat ttattttagc ccaacaatac aaaacatact tccaactggt ggagatagca
     1621 gaaaacaggc tacaatgata ttctcagttg ctatattagg atgcgctgtc tttactacat
     1681 acgcttcttc cctcttttta aaatttaaaa gcaaggaaat tggtattttc atggctctag
     1741 gaacagataa gaagaaatta aaaaaacttc tattttcaga gatattgtta atttcaatag
     1801 tatgctgttt tttaggtctt atattaagta ttcctgtatc ttttggtata tggaaaatat
     1861 ttcaattagt gattattgat accagagaaa tgacatatca ttttggaact caaggattca
     1921 tatatggatt tatatttagt gtatttgtga cattatgcat atttatactt tctgtaaaat
     1981 ttattaatcg tacaaatata attgatatat taaatgacca gagaatatct gaaccaatac
     2041 aggatgtaaa ggactggtat ggtatattag gtataatttt tattatacta ggtatttttc
     2101 taggatatat cgtaccttca ctaactataa acaaattaaa ttatagacta ccatctgtat
     2161 ggagctttac ttatctactc tctcttattg gaatatatat gattatgaca tacatagttg
     2221 tacatgctaa aagaggaaaa catccagaaa aatactataa aaacatcata tcaactagca
     2281 tgatgagatt tatgggaaga caaacagtaa aaaacatgtg tgttatttca tttttgattg
     2341 caggagcatt atttgctgta ttctatgttc ctaatatggt tgcaggtatc tatgatactc
     2401 ttaaaaataa ccctatagat tatactttta cctacaatca aagagataaa caggtatcaa
     2461 aaaaagatat tgaacaatta gcagataaac acaatataaa aattcaaaac tatatggaaa
     2521 ttccttctat agaattaatt gttgatggtg tagaatcaat atatcataaa gatggaacta
     2581 tcgaaagggt atatatgaaa aaaagctcct atgatatgtt ctttaattct aaagattttt
     2641 ccaagctaat tggcaaacca ataactataa aaaatggtga atatcttgct gttgtaaata
     2701 aaggaaatga aaatcaagat tataaatata ccaaatttat atcaagccca gtcacagaaa
     2761 aagaaatccc ctttagatat gctggaaaaa taatatttaa tggttctttt tttcaaaata
     2821 gaagtatgaa cgcctatgtc cttaatgatg aggattataa atcatttact aaagatttat
     2881 ccataaaaaa ttacaataat tctatactat ttaatgtaga taaagatacc tatgcttttg
     2941 ccaatgactt aaagaaagaa attataaaaa gaacatctaa ggaaatgggc atgttcagtg
     3001 gttatgatga atacgtaaaa aaacgttatg aatcaactgg aagaaaatat tttatggatg
     3061 aagaatatcc aagtggtgaa ggtcatttaa aactagaccc aaacaacaat cagttgtatc
     3121 ttaattggaa atactatcct aactttaaaa ttttacaaag ccaagatatg ataaaaaaca
     3181 tggcagtctt cttaatgtta ttcatatatg tggcaatcat atgttttaca gcagtaggaa
     3241 tcattgcata tacaaggggt gttactatag ctataaatca caagcaggtc tttatggatt
     3301 taaatcgatt aggagcaaac aatgattata taaagttctg tattagaaat caattaaaga
     3361 aagtattttc ataccctctt attgcaggaa gttttttaat ttatctgttt aattttatga
     3421 ttatgttaaa taatgatggt agaatcgtca atagtgaggt caatgctctt ttgataaact
     3481 taggttttat agggatttgt ggggtttata tgtaccttat atatctatta acttacaaga
     3541 aattcaaaaa aattattggt attttataat aaggaaggtt gttagatttt taaagtaata
     3601 aaatcattgg tgttttataa taaaaaaggt tattagattt ttaaagtaat aaaatcattg
     3661 gtgttttata ataaaaaagg ttattagatt tttaaagtaa taaaatcatt actttttata
     3721 ataataaaag catttgtatt ttataataag aaagattgtt agatttttta ataagaaaat
     3781 cattagcact tcagaataag tttaatagaa tagttttaag tgtaagtata ctaatataaa
     3841 aaattttatt ttgctatctg gatataattg ttatttcatt ttatatatct aggtagcaaa
     3901 atatattata tacttaacta tataaatata aaatttgaaa ggagataaaa aattgacttc
     3961 gatttgtatt tgtgatgatg aaaaacaaca aagaaacatg ctgaataaaa tcattacaag
     4021 agaaatggat ttgtggggct acccctataa aataaaagaa tattcaaatg gagaaagtct
     4081 agtatacaat ctagaacacg aaaattatta catagatatt atttttttag atattgaact
     4141 tggaaaaagt aatggcgttg acatagcaaa aactatacga aaacattcaa aagatactat
     4201 aatcatcttt gtcacaggtt tttctgacta tgtatttcat ggatatgatg taggagcttt
     4261 gaactatatc ttaaaacctt ataaaactga aaaaatatgt aaagtattaa aagaagcact
     4321 aaaaaaatta gaacaatgga aagaaaactt tattacagtt cagattggaa ctaatctttg
     4381 taaaattaat acaaaagata tcctctactt taaaagtgag ctaagaaaat taactgtagt
     4441 tacctttgat aaaacattag aatactacgg aaaattaaat gatatggaaa ctgttcttcc
     4501 ctctaatttt atacgtacgc atcaaaggta tattgtcaat ctaaaccatg tagatagtat
     4561 aactcaaact tatgccaaag ttcagaatga aaaaattcct ataagccgta aaaaatatca
     4621 agaggttgta ggaaaattta caaggtatat gttagaacct taatttttga agttcaatct
     4681 ctagaatttt atttttgaaa cttaactttt taaagcttag tttttagagt tttgtttttt
     4741 caaatttaat tttttgaaga ttaatttcta gaatttcaat ctttcaaaat ttaatttttt
     4801 gaagcttagt ttttagagtt ttgttttttc aaacttaatt ttttaaagct cagtttctag
     4861 aatttcaatc tttcaaaatt taattttttg aagcttagtt tttagagttt tgttttttca
     4921 aacttaattt tttaaagctt agtttctaga atttcaatct ttcaaaattt aattttttga
     4981 agcttagttt ttagagtttt ttttcaaatt taattttttg aaacttaatt tttaaaattt
     5041 caatctttta aaactcaatt ttttgaagct tagtttttag agtttttttt caaatttaat
     5101 tttttgaaat ttaatttttt aaaacttagt ttccagaatc ttaattaata gaaaggtata
     5161 ttaaatgggc acttattctt taatctctac aagcataaat tttattctaa tattaatcaa
     5221 tattttcata gccttttttc tttatacgtt gacttcctat tttatagact ttaggaatca
     5281 ttggattttc aaaatcatat tgattttggg ctactctatt attagtcaaa tgataatatg
     5341 gctttccgac cccgtaaata ttttgtttac actattcgga tattttatac tacttataat
     5401 atgtagtaaa agcaaattaa ttcctataat atcagttgtc atgatacttt atcctattat
     5461 aattgggctt aattttcttt ttataaacaa ccctatatat gaggaactta tacactcaaa
     5521 agagtatatt ttaatatttt caatagtcca gataatttct gctctaatta aattattatt
     5581 ttggttttta atacatattt tcctaaagaa tagattggtc aatataaaac aatacttatc
     5641 aaataaaata tggattttta ttgatatggt atgtatatct tcatttttat caatattgat
     5701 tgctattatt ctgccaccta ctaatttaat ccctatgggt agtaataaga atattatctt
     5761 aggcaactta ccttatggca cttatctaat catcttgtca gcaattctgg caaatatcgg
     5821 tgtgatactt cttttacagc cattgattga aaatgtaaag atgaagatag aaaaacaagc
     5881 aaatcaccta aaagaaaaat actataactt actagaaaat caacaaattc aaatacgcaa
     5941 aatgcgacat gatatgaata atcactttca aatgattgaa tcgtatttag aaattgaaga
     6001 tattgctgga gctaaaaagt attttaatca gttaaaaata ggtataggta ctcttagtgg
     6061 taaacaattt tgcaaaaacc aagctcttaa ctcaatttta aatcttcgtc atacaagttt
     6121 ggaagaaaat ggtgttgatg tgcactttaa tatagatata gacaatcact tgggtataga
     6181 acctatagat ttatgtacta ttttttcaaa ttctttagat aacgctatag aagcatcttt
     6241 gaaaattcct gatttgtcaa atagaaaagt ggtattaaag gcacgttgtg agaaggaata
     6301 ttttagctat ttattgacaa acaacaaagt aaatcctatc aatagaaaac aagaactatt
     6361 tatttcagat aaaaaagata gtaattctca cggatatggt attgagaata ttagagaaat
     6421 tgttaataag tacaatggaa aattggatat ctcttatact gagagtgaat tttctttatt
     6481 tctttatatt agattatttt agtctaaaat tagattgttt gatactatgt aaaattataa
     6541 taacttataa ttaaatattt acacatatat tcattaactt aaaatttaat caaatgtgat
     6601 attttattta ccttagtaaa aatgtgtgtg tctcaaaata aactttttag ttgatgaggc
     6661 actctttttt acatgaaatc aaatatattt ttatcctttc aacaatgaaa aagggattta
     6721 tctctccatt ttgaaacaag ccctttttct attaattact ttcttaaaaa atataatacg
     6781 atatataaga taaatatcta attgtaccta tttctaatta tatctattag aatacaacac
     6841 ctttgccttt tcagtaaact ccctaacaaa atctagttta ctatctgtat aagcatcacg
     6901 attatgctca tacttcttcc aaaggtccaa cttaagtttt tcatactcct ttgcaacatc
     6961 tgggtggtca attaagtaat ctctaaaaaa caactcatca ttatcaccta cataccttat
     7021 atgcaaatga aaaactcgtt cttcaaaccc atctactgta taccctttat taagtgaaag
     7081 cctactactg ctctcagaca tacaaatata tccattcgac tcaatcaaat ctttaatatc
     7141 tttcatatta taatcaggaa ttatctcaat cagtatgtca acaatgggct tggcataaat
     7201 cgtactcaca gaagtacttc caatatgact tattctaaca attttttgca ttggcaatat
     7261 tccagttagt ctatattgtt cttctttaaa ccaatttttc caacattctt tgtgagtagt
     7321 caaaaatata ggaaacaagt tccataactc ctctaaagtc atatcttcca acttctttct
     7381 cattagttac ctccttttaa atttgtactt tatatttttt ctttttcaaa tttacttagt
     7441 taatatttaa ctttattttt tatcaaatca tttttctaat tatgtaaatt tttctaaatt
     7501 tagtttttaa tgtattataa cagattttta cccttcttac acttttgcat ttctttaaat
     7561 attagaacat ttttaatcta caattgtagt aattttttct ttacctgtat ttactggcat
     7621 tggagcttct aaagaataat tcattggctt taatataatt aaagtaaatt taacatatgt
     7681 gaaagatact tattaaaatt atttatatta atctcttgtt aaactttgta ttttttaatt
     7741 atctatataa acattaacct gatatgtagg ttttgaatta gttctgtttt caataggtgt
     7801 ttgttttgaa agtggataaa atattgattt agtagtaatt aaatcaattt taaaatatgt
     7861 aatagaagat aataaacata aaataactgc tatcaaaata ccatttctgt acttttcatt
     7921 aaacatacta aacactcttt ttttaatatc tgactcctta tgtaatccag ccacaaattt
     7981 atttgaacta tttgaacctt ttctcattgc actaattatt gtcaatgcat aatcctgctt
     8041 ctcttctatc gtacaattct tcaaaactct ttcatcacaa taaaattcac aatccaaatc
     8101 tataaacttc tccattatat acacaaaagg attaaaccaa taaacacatt ttaaaaatat
     8161 caccaaatac ttaatataaa ggtctttact cttaaaatgc atcaactcat gcctaaatat
     8221 ccagtttaac tcttttaatg tataatcaca tggtggtaaa atgacacacg aattaaaaaa
     8281 tactggactt tcaacttcat atgaatattt aagccgtatt tttttattta tcttaagctc
     8341 ttttaataaa ttttcataaa gattttttat cttatcatcc tcaacatcat aagacaaatc
     8401 atttatcata tttttaagct taatatattt caataaagta taaactaaat aaaatatcgt
     8461 aatagcaatc caaacagaga ctaaaacagt actatataca atagaaatac tattaaaatt
     8521 gcctaaactt atcttattaa tattaccatt atattttgac tcattaaata taaaagtata
     8581 attcataaat ggtaatgata gcttaataat cacaataagc catatatagt aattaaattt
     8641 tttactgaaa gtcttaaata aagttattct aaagactaat aaaataacta taaaaataca
     8701 acttactaaa attgttctaa agatatcatt taaaaatgaa ctaaccacag atactcacct
     8761 ttcttatact tttatattta tataatcatc tacacgaata ctaaactgat atgtaggttt
     8821 ttaattaact ttgttttcaa taggcgattg tcttgaaagt gttttaaaag ttgatataac
     8881 aatgatttag gagtaataag gtcaatttta aaatatgtaa taaaatataa taagcatgaa
     8941 acaactacta tcaagatacc atttccaaac tctataagca aaaggggcta atacaacctt
     9001 aacccctttt aattattttc atttataaat aaattctatt cttcccaaaa tatcgcaatt
     9061 aaaatatatt aattaaaata tcccaataag aatatctgaa aaatcttcta cctatttttt
     9121 caaactcttt attcccattc ctcatacaat agtgcaactt ttagcacagt ataaatatac
     9181 cattagttca tataatctca ttaacaaata tatacaatta ttttcttcaa actcttttac
     9241 atctaagata tcacattttc attttcttat attaattcct taactctctt tcaaattacc
     9301 aattttttaa ttatcaaatc ttcattaaat attctatcat tattaaacat tcatcattat
     9361 ccctaaaaaa tatgtgacgt atgttcttta cacaaatttt ccatcttatc atacacagca
     9421 ccactatctc tctcatctgt catagtcaca attacatcac ttggtctaag aattgtctta
     9481 cctctaggaa taatttcttc tccacctctt tgaattgcta ccaaaagaca attttcaggc
     9541 cactgtacat cctgaattgc cttgttcgca acaggagagc caaacataac agagaactgg
     9601 tctagtatct tttcgcccct ctctttatgt acaggttctc catttttttc aagtaacctc
     9661 tcaagaagac tttcataaat aggtttagac ttcataagtg tagccacaat atatgccaca
     9721 attgaaatta ctgatagtga aagcatttga ctaacagaac ctgtcatttc aaaaattaat
     9781 atgataccag tcaaaggggc acgcacaata gctgtaaaat atcctgccat agccaaaagc
     9841 acaaagttat ttacataatc gggattcatt ccaaaaactt taacacctac cataccaaat
     9901 attccaccta taaatgctcc taaaacaagc aatggaaaaa atattccccc tggtgcacct
     9961 gacccaaaac taacagccga aaatataaaa cgtataacaa aaatcaaaac aaccattcca
    10021 aaaatatatt catgacttgt aagttcttca actaaattgt gtccactccc cagaacatca
    10081 ggcataacaa gcccaagaat accagccatt ataaatggaa tcaacagttt tccaaacgta
    10141 ccaattttag gaatcttctt gtataaagac tgcacatata gagtaaacca gttgtaaaat
    10201 gcccccataa ctccaagaat aacacctaaa atcactatta atccataata atcttgtggc
    10261 aacacatttc caatctgaac ctcccatgac taaagtcaca gggttcctgc ttcatagaat
    10321 tttgcatact aaaatatgtc ttactaattc tccacaggct atccccgtag ttcctacggt
    10381 tcttacataa gttatttatt ttgaattgtt attagtctta atccttcttt taatatattt
    10441 atacttgcat ttatatctct atcgtgagtt tcattacaac taggacaaat ccattctctt
    10501 ataataatgt taacaaatgt aattatttaa aacaattatt tagaaggttt tatatttaat
    10561 tttaaataac atatttaaga accgtaagtc ttaactttta tcatttttga ggttgtcgtt
    10621 cacataagtt cgctactacc tatgcagttc tcttatgaac ttcttacact ttcatgcaag
    10681 cacagactat atcttatcct tcggcattat ccgttaaggt ctacccactt ccactaccaa
    10741 tagcttgtag tgtacttccc tcaagaggaa tagtcgttga agtttctcct attcggagct
    10801 tacctgctga ttgcccattt ttaaagtact taggatttaa ccttatacca tctaaccaat
    10861 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
    10921 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
    10981 tataggtttc cctatatact gactatttta gctactaact catgactaaa gtcataagtg
    11041 tgcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
    11101 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
    11161 ttatggactt cttcaagtga aaacataaca cccgccagtg gtgcatgaaa ggctgctgat
    11221 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
    11281 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttctcttccc
    11341 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
    11401 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
    11461 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
    11521 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
    11581 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
    11641 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
    11701 tgagatgtat cctttttcat actgttcctc cttcatccaa taattctaca caatctgaat
    11761 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
    11821 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtaaa tttattcttt
    11881 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattag ttcttgcttt
    11941 ctctgtgtat agattttacg attgtcctca aactgtaatg ccaatttttg tttatgacta
    12001 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
    12061 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
    12121 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
    12181 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
    12241 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
    12301 tcaatggcag tcttatccca tatacttttc aataatataa tgacatcttt tgcactttta
    12361 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
    12421 taccacctca atataatcca cctttatata tattacaatt ttttctctct tgataaacag
    12481 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
    12541 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
    12601 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
    12661 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
    12721 aacccttttt gacagacaca atcctttatt ttacacttac ggcagtgttc tggctttcct
    12781 gtatcgccat tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
    12841 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
    12901 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
    12961 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
    13021 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
    13081 atacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
    13141 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
    13201 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
    13261 gttttgaata gcagataatt atatgattaa aaattaaata tgacatagcg tttgtggaat
    13321 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
    13381 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
    13441 gtatcgaagg tactgttaat ggcatgcagg gtaatattaa ctatatgcaa ggtactatta
    13501 gcaatatgca aggcagtatc aatggtatcg aaggcactat tagcgatatg caaagcaata
    13561 tcaatggtat cgaagacact attagcgata tgcaaagcaa tatcaatggt atcgaaggca
    13621 ctattagcga tatgcaaagc aatatcaatg gtatcgaagg cactattagt agtatgcaag
    13681 gcaatatcaa tggtatcgaa aacactatta gtggcatgca aggcactatt ggcgatatgc
    13741 aaggtagtat cagtggtatg aatggcacta ttagcgatat gcaaggcagt atcagtagta
    13801 tggatggcac tattaatagt atgcaagaca gtattactga tatgcaagtt gaaattaaag
    13861 aagtcaatag taggttagac aatatggaac ctgatattaa agacttaaaa actggacaag
    13921 ttgaaactag tagtaaactt gatactattc agagtgatgt tacagaaatt aagtcagatg
    13981 taaaaattaa cagaaataca ttagatacag tgattaaaca tacctcttca ttagtaaaag
    14041 atacatcaga tattaaaaat gatttaaata atatagagat agtcacatct aagaattcga
    14101 atgatattgc taaattaaaa tcagttaaat aatatactat agtaaaaaat acctgaaaat
    14161 ttcacatact tctttaaagt tataccaaat tttatacaga aatactgatt atccgacaac
    14221 acactttact tataaataat ttattaaaaa aatactcatt ttatcctaaa attaacacga
    14281 ctaaatgagt cttgatataa taataaaaaa taagggagat tgaacactaa aaagttgtgt
    14341 atctccctta tattctataa tatagttata taatttttca gcctaaaata aaagtccatc
    14401 tttgattaaa acacatcccc ttatcactta taaaaggata aaatcttgtt ttcatttttc
    14461 catttatatc ttcttgctta agagtaataa ttttttcttt tgatacttta agtgtttggt
    14521 taaatttaac caatcgacta agttttgttc aacatctatt tttcatattt tccaatactt
    14581 aatctatttt ttataaataa ttcttttatt tctaactcac ttacattttt tacataaaac
    14641 gaaacatata aatttcaacc ttatcttgta tggttcttaa tacttaatga gtagcttaag
    14701 tatcaaacat tataaatttt ccccttacat ttaacactct taccatttcc aaaatataca
    14761 aaatatattt tgaacatttg tacaatgatg aaatacaata catgaaaata caatatcata
    14821 actcttgtct aaaaatagaa gttcttcaac ataacctttc acaaaaaaca tattatctaa
    14881 gtattttttg tctgctcctt gttttcctgt ttgtagcata taaattactt catctaaaca
    14941 tataactact cacacgaaaa aagaaaataa acttccaaaa tacaagctct aaaaactacc
    15001 tttaaatttg tataatgttt actaggaata aggttgtata acatatagtt taaataatca
    15061 ttttttaaaa ttgtaagtta ttgctttcaa aattagatgt ttgtatatca aataaatgat
    15121 taagaatttc taaatttaat ggttttataa tgcactccct ttctttggga taaaataatg
    15181 ttgcacagat actttttcta aagataacaa ttttattttt ctatcaattc caccagcata
    15241 cccagtcaaa ctcccatttg ttcctacaac acgatgacat ggaataataa tagagattcc
    15301 attatgccct actgcaccac cgattgcttg tgctgacatt ttagatattc ctttttgttg
    15361 agcaacttta tatgcaattt ctccataagt gattgtttta ccatatggta tttgttgtaa
    15421 aattttccac actgtagtct gaaaaggagt acctaccata tggagtgatg gtgtaaaatt
    15481 gggttctttt cccgaaaaat aaatatctaa ccatttcttt gtctgctcga aaattggcaa
    15541 ttctttttct tgatgttctt ggtcaagatt ataagcataa tacttttgat tctcaaacca
    15601 aagaccagtt aacccaatat tatctgatgc taaaagaata tttccaattg gactatggta
    15661 atagctaata tattgcatac aattcctcct tatattctga aaacttcact caaatcatta
    15721 gacacctctt ttctatctac cattatgtaa aaacatacta atataatttc cttctaacta
    15781 cactttcgtt ataatatcta tcctcatatc atattagttt tatatttacc tgcatctgtt
    15841 catccttttt tccctttccc tttgatgaat ataaaattca tacatttaag cttcctactg
    15901 tattaacttt tatagaagtg tatattataa ttttatcacc aaatagacca aaaaaatatc
    15961 gatatagcat caagtaatat cataatactt tcaaatggga aaagtaagaa taggctgtaa
    16021 aaaagaaaag tatttttatg ttagaaatcc agtaaaatcg ttagtagata gaataacaga
    16081 aaacgaacaa tttgctattt atcattttac acataaatat aattggcgca ataaagtatt
    16141 ttctattcta caacgattaa taaggttaga aaaaagtaaa aatgaactgt atatgtatga
    16201 agcattagtt tctgtttctt tcctttggct tattatgata aaaaatattg ttttgccacc
    16261 aataaaacac aaaaatataa ttaatatcca tatgcaaaaa attttacgtt atattgaaaa
    16321 atactattgt tttacattat attgaagggt actattgtga aaacttaatt ttggagtact
    16381 atcaagcagt ggtaatatca ataaatcaga gtgctccaga tgatttgaac taagtttaaa
    16441 tactatacct tataaatatt ttactgagtt ttgtttatta aaagcaactc aatttctaaa
    16501 aggcacaaat gagctaataa gtgacattac tataaaagtt gtttttcatc aaataatcta
    16561 ttttagtaaa tgttttaaag aaaaaacggg atattctcca aaagtgtaca gaaatataaa
    16621 aaaagagata tgatttttta tttcttttga ttatccaaag agtaaataaa aaatattgta
    16681 aaaaacttac taacaacggc aatgctttga actatggtaa tactttgaac ttttgattgg
    16741 actttttttg ctaaaataat atatttaaat agcaaagtaa tctgtttaat caataaaaaa
    16801 taagagataa aagaaatcaa ctaaaacttt tacctcttat tttgatagtt tatcagagct
    16861 aatatttaca ctctttttat ttttgatact tcattaaaaa tgatgcttac tctccattca
    16921 cttttaattc ttcattaaaa atgatgctta ttctccattc acttttaatt cttcattaaa
    16981 aatgatgctt actctccatt cgcttttaat tcttcattaa aaatgatgct tattctccat
    17041 tcacttttaa ttcttcatta aaaatgatgc ttactctcca ttcgctttta attcttcatt
    17101 agaactatgc ttactctcca ttcactttta attcttcatt aaaaatgatg cttactctcc
    17161 attcaatttt gatacttcat taaaaatgat gcttattctc ttttcacttt taattcttca
    17221 ttagaaatga tgcttattct ccattcactt ttaattcttc attaaaaatg atgcttactc
    17281 tctgttcact tttaattctt cattaaaaat gatgcttgca ctccattcac ttttaattct
    17341 tcattaaaaa tgatgcttac tctccattca attttgatac ttcattaaaa atgatgctta
    17401 ttctccattc acttttaatt cttcattaaa aatgatgctt gcactctgta aactcataaa
    17461 catccattaa tgcttcacca aatctttgat agtgaactac ttccctctca cccaaaaatc
    17521 tcaatatatc tttcaaatca gggtcatcag ttaaatttat taattgatag tacgtagcca
    17581 atgctttttg ttcagcagcc atatcctcat gtaaatcagt taccgaatcg ccaaagacat
    17641 taatataact tgtagtaaag tttactccat tagaatctac tggctgaaga ccatgtccaa
    17701 aattagcata attactacca agtccagcag ccttaagttc ttctggagta caattgccta
    17761 ttaattgata taacactgaa gaaattatct caacatgagc catttcctct gtacctatat
    17821 cagttaaaag tgcacgagat tttccagttg gcatagtata tctttgttga agatatctag
    17881 ttgcagctgc caactcacca ttaggtcctc ccaactgagt catgatatat tttgccattc
    17941 tagggtctgc ttgtcttatg ttaactggat gttccagtgt tttttgataa atccacatat
    18001 tatcttactc ctccttaata tatatttaat aataaatcta attttaaagt ttaataattt
    18061 tatttataaa atgatttgtc ccatggccaa ggttcagata tccatgacca aggatattca
    18121 cttgattcat atccaaaatt agttaatgct ccatatttag attcataatc acacacagct
    18181 tgtgtaaatt tatcgcagta tctgttgtat gaatcaagag ctttcttatc ccttggattg
    18241 ttatctaaat ataaatttaa ttctatacag gcaaattgat attctgaaat tctctctaaa
    18301 agttctcttc tatttaatga cattagtaca tgttaccccc ttttttagct ctaactctat
    18361 ttttaggatt tgcatatagc caatcattat agttatttga ctctattaaa tctaattcag
    18421 gaaataaagt acctcttttt aatgctgttt ttggattgta tgtgtcatca taaatttgac
    18481 gattgatgta tggtctagca aattcacaac aatagtcttg tttatcctct tttaaatatc
    18541 ccattaatta tcctccttaa tttttagaat tttaaaatag cattattttt aaaaattgta
    18601 tactggttct atattataac tggtagtttt aataggtctt taataagatt aaatgattaa
    18661 ataagtatct ttttttctta ttttacctct aaaatttttc taattagtct ttcataaata
    18721 tctattttaa ataaataata tagagtttat ac
//
LOCUS       DAEMHZ010000070        18674 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000070 DAEMHZ010000000
VERSION     DAEMHZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.070"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..249
                     /locus_tag="KPK72_002286"
     CDS             <1..249
                     /locus_tag="KPK72_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2489495.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIG
                     FGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            265..1848
                     /locus_tag="KPK72_002287"
     CDS             265..1848
                     /locus_tag="KPK72_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG2489496.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIRFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEVVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1876..3204
                     /gene="ssnA"
                     /locus_tag="KPK72_002288"
     CDS             1876..3204
                     /gene="ssnA"
                     /locus_tag="KPK72_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG2489497.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3403..4755
                     /locus_tag="KPK72_002289"
     CDS             3403..4755
                     /locus_tag="KPK72_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2489498.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4947..6056
                     /locus_tag="KPK72_002290"
     CDS             4947..6056
                     /locus_tag="KPK72_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2489499.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6339..7730
                     /gene="hydA"
                     /locus_tag="KPK72_002291"
     CDS             6339..7730
                     /gene="hydA"
                     /locus_tag="KPK72_002291"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2489500.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7724..10285
                     /gene="xdh"
                     /locus_tag="KPK72_002292"
     CDS             7724..10285
                     /gene="xdh"
                     /locus_tag="KPK72_002292"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG2489501.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10437..11783
                     /gene="rpoN"
                     /locus_tag="KPK72_002293"
     CDS             10437..11783
                     /gene="rpoN"
                     /locus_tag="KPK72_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG2489502.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12581..13603
                     /locus_tag="KPK72_002294"
     CDS             12581..13603
                     /locus_tag="KPK72_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2489503.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13663..14670
                     /gene="gap"
                     /locus_tag="KPK72_002295"
     CDS             13663..14670
                     /gene="gap"
                     /locus_tag="KPK72_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2489504.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14892..16094
                     /locus_tag="KPK72_002296"
     CDS             14892..16094
                     /locus_tag="KPK72_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG2489505.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16120..16863
                     /locus_tag="KPK72_002297"
     CDS             16120..16863
                     /locus_tag="KPK72_002297"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG2489506.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16872..18404
                     /locus_tag="KPK72_002298"
     CDS             16872..18404
                     /locus_tag="KPK72_002298"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG2489507.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7496 a         2157 c         3753 g         5268 t
ORIGIN      
        1 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
       61 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
      121 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
      181 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
      241 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatggact tatagtagat
      301 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
      361 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
      421 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
      481 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
      541 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtttagat
      601 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
      661 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
      721 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
      781 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
      841 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatac
      901 gatggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
      961 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
     1021 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
     1081 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
     1141 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
     1201 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
     1261 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
     1321 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
     1381 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
     1441 ttctatggat tagaagaaca tataataaga ttcatgaaaa gagacgagca aaatgtgtgt
     1501 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
     1561 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagttgt ttataaaatg
     1621 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
     1681 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
     1741 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
     1801 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
     1861 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
     1921 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
     1981 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
     2041 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
     2101 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaacaa tttatggtgg
     2161 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
     2221 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
     2281 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacatgtctt
     2341 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
     2401 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
     2461 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
     2521 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
     2581 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
     2641 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
     2701 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
     2761 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
     2821 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
     2881 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
     2941 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
     3001 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
     3061 tttggaatga gtggaagaag cgtagataca actattataa atggaaaggt acttatgaaa
     3121 gatagaaagt taataaacat agatgaagaa agtattctag caaaaagtag agaattaagt
     3181 caaaatctat ggaatagaat ttaaataatt gattatgcaa aattctatga agcaggaacc
     3241 ctgtgacttt agtcatggga ggttcagatt aatatacaag taagaaacaa gaatttagat
     3301 gttaaaaaca gagcttaaac aaaaatagga atttaaatga agtatataaa taaaaaatat
     3361 acggaataga atattaaatt aaatatagaa gggagaccca aaatgtctcg agataaaagc
     3421 cctaaaatag ctccagtaga tgaaaggtta cctgtatcca aatcttgggt attttcacta
     3481 cagcacgttt tagcaatgtg tgcaggagca gtagcagttc ctatgatgat aggaagtgct
     3541 gcacgactag atcatgctag tattgttttt ttaataaatg cttgcttgtt tatggcaggt
     3601 ataggaacac tcatacaaag tgttggtata ggaaatattg taggtgctaa aataccagta
     3661 atagaaggaa ctagttttgc cgctgtggca ggtatgacag caatagcctc atcatatagt
     3721 gattcacaag ttgcaataca gacagtattt ggctctgtaa tagtagctgg attgttttgt
     3781 atattacttg ctccagtatt tgaaaaatta ataagatttt tccctagtgt agttactgga
     3841 acagttgtta tggtaatagg tatttcacta tttccagttg ggataaaatg gataactgat
     3901 aacaaattgg aagcggttga accaaactca ttagcattag ctttagcagt attggcaata
     3961 actttacttt catttaaatt cttaaaagga atttggaata gcacagctat attggtaggt
     4021 atagtacttg gtacactact ttccatggca gttggaatgg cagattttag tgcagtaaat
     4081 ggagttggtt ggcttaactt aaatgtacct ttaaaatttg gaatgcctag atttgaacca
     4141 tctgcaatac tatcattgtg tttgattatg ttggtactta tgacggaatc agttggtaat
     4201 atgatagcta ttcatgaaat ggtagacaaa gaagtaacag gtaaaaatat taaaagaggt
     4261 ctatttggag atggaatatc tacagcatta tctggaatat tcaattcatt tccaataact
     4321 ccatttggtc aaaatacagg aatagtaggt cttacaggta taaaatcaag atttatagca
     4381 gtttacgcag gggttatatt gctagtactt ggattcttac caaaatttgc agcaataata
     4441 ggagcaatac ctaagcctgt tcttggaggc gtaggatttg caatgtttgg aatggtaatg
     4501 gttggaggag taaaaacttt aagcaaagtg aaatttgatg gaactaaaaa tggggttata
     4561 gtagcagtta gtgttggttt gtctatgata ccactaacaa atcctgattt ctatcataat
     4621 ttcccaactt gggtacaaac aatattccat agtggaataa caacaggtag tttatcagca
     4681 gtgcttttaa acatattctt caatgaacta ggttctaaaa aagaaaaaaa taataaagtt
     4741 gaagaagcaa aataaaaata aaacaagtaa aaatctatat tgacttaaag tcattaagca
     4801 ttaataaaaa taattcagag atattaataa ttcaacaaaa ctatcttata ttttaaataa
     4861 tataaaaaag taaataaata acgaatacaa ataaaagaat acaatctaaa tattaaaaat
     4921 atttaattta agggagagat aataatatgg ctaatttaaa agttaattta tatgggatgg
     4981 aatttttaaa cccaattatg acagcggcag gacctggagc aaaagatggt gctctctgtc
     5041 aagaggcagc aaaaggtgga gcaggagcta tatgtacaaa aactatatct gtattaccag
     5101 ctgatgtacc aagaccatgt atggcaaaaa ctaatagtgg ttttttaaac acagaattat
     5161 ggtcagagct tccaaaagag caatggatag aggaagaata caaaaaggta aaagaaacag
     5221 ggcttccagt aatagtaagt atgggatatt cagcagagca aatagctgag gttgctcctt
     5281 tagtaaagcc ttatgcagat gcagttgaat tatctactca ttatgtaggt acagacgttg
     5341 caccaatagt aaatgcttta aaagctgcaa aagctgtttt agatgtacca gtatttatga
     5401 aaatgagtcc tcatactgac attcaaacta ttgcaaaagc tgttgaagaa gcaggtgcag
     5461 atggacttgt aatgataaac tcatttggac catgtatggc aatagatgta aatacaggat
     5521 accctataat gggaagtaaa gctggatacg gatggttatc aggtgcacca ataagacctc
     5581 tagcaataag atgtatatat gaagcttctc aagttgtaaa tataccaata atcggtgttg
     5641 gtggaatcac ttgtggaaaa gacgtggctg aaatgatgat ggctggagca agtgctgttc
     5701 aagtatgtac agaagcaatc cttaaagggc cttctatcta tggaaaaatt gctaaagaat
     5761 taaatgaatt tttagatgag aatggatatg ctgatgttaa tgaaataaaa ggattaactc
     5821 ataagaaaat ggcagctaga gggtttagaa ctcattcagt agctccagta gtggatgata
     5881 acaagtgtat caaatgtaag caatgtgtaa caagctgtgt atacgaagct ttagaagtta
     5941 cagataagtt gaacatagat gcagacaagt gttttggatg tggattatgt gtaactagat
     6001 gtccaaaagg agctttaagc atctgctaca atcaagtaga agctatgaat gagtaataat
     6061 tatatagaat tttggtaatt aacttagagt aataggtatt aggataataa aatatatcag
     6121 ataaacaaac acacattatg cattaataat acagtctaaa gttaataatt ttattatatt
     6181 gatttaacta aatattagtt tagctgaata ttaatttaat taggtattgg tttaatctta
     6241 atatagattt actacgtata agtgaactaa ctagattact ttaagttaat tttttcatat
     6301 aaaataaatt gatacattac aagtaaggtg gagtctatat gggaattgtt ttaaaaggtg
     6361 ggaaagtagt aacaagtgaa aaaacttact gtgcagatgt aagaatagaa gatgaaaaaa
     6421 tagtcgatat aggtcttgat atatcaaaag aaaatgatga aatagttgat gttagtgatt
     6481 cttatgttat accaggaggt atagatacac acacacactt tgacttagat acaggcgtta
     6541 ctaagacagc agataacttt gagacaggaa caaaagctgc tatagtagga ggaactacta
     6601 ctatattgga ttttgctaca caagctaaag gatgttcttt gaaagacgga ttagaagaat
     6661 ggcatgaaaa agctaaggat aaatcctatt cagattatgg ctttcatatg gctataacag
     6721 attggaatga ctcggtttgt aatgaaatgg aggatatggt gaaagaggga gtatcttcat
     6781 ttaaactgta tatggcgtat aaaggaagcc ttcaagttga tgatggtgta atctttgaag
     6841 cattgagaaa agcagaagag ataggaggga ttattggttt ccattgtgaa aatggggaca
     6901 taatatgtga acttgtggat aaagcaaagt cagaaaataa tttatctcca aaatatcatc
     6961 aattaactag accagctata atggaggcag aagctacatc aagacttatg aagatagctg
     7021 aagttgcaaa ctctaaagta tatgtagttc acttaagctg taaagaatcc ttagaggagg
     7081 ttttaaaagc taagcaaaga ggtgtggatg taatagttga aacttgtcct caataccttc
     7141 tattaaatga tgaattatat gatttagatg gatttgaaag tgctaagtat gtaatgtcac
     7201 cacctttaag aaataaagaa aataatgaaa tattatggaa tgctatttta gataatcaaa
     7261 tagacactat aggaacagac cattgttctt ttaattataa gggacagaag gaacttggga
     7321 taaacgattt cagtaagata ccaaatggtt ctccaggtgt agaacatagg atgagtcttt
     7381 tatacacata tggagtctgt aaaaataaaa tatctatcaa caagatggta gaagtaacat
     7441 caactaatcc agcaaagttg tttggaatgt atcctaataa aggaactata gaaataggca
     7501 gtgatgcaga tatattagtc ttaaatccaa acaaaaaatc caaaatagaa gcgaaaaaac
     7561 aattacaaaa tgtagattat acaccttatg aagggtatga agtggattgt caaatagaac
     7621 atgtttattt aagaggaaaa gaagtagtta gagatggaga attaatttgt aatactccaa
     7681 gtgggagata tataaatagg cgtctaacaa agggggctag agaatggtag aatttatatt
     7741 aaatggagag aaagttgttg caaaagaagg caaaaaactt ttagacttcc ttagagaaga
     7801 tatgaatata acttcagtta aaaatggatg ttcagaaggt gcttgtggaa catgtatggt
     7861 tataatagat ggtaaagcta ctaaagcatg tgtatttaaa actgataaat tagctgggaa
     7921 aaacataaca actatagagg gattatcaga gagagaaaga gatgtttttg catattcttt
     7981 tgctagtcaa ggagctgtac aatgtggatt ttgtattcca ggtatggtaa ttagttcaaa
     8041 agcacttata gacgttaata ataatcctac tgaagatgat ataaagaaag ctttaaaagg
     8101 taatatatgt agatgtacag ggtatgtaaa aataattaaa gctgtcgatt tagcagcaaa
     8161 gatttttaga gaaaatatag atgtgccaaa agtaaactgt aagggtctag taggtgaaaa
     8221 tcttcctaga gttgatgcag ttggcaaagc acttggatat tctgaatatg tagatgatat
     8281 gagaattgag ggtatgattt atggttcagc agttagagct aagtatccta gagctttagt
     8341 taaaaaaata gatatagaag aagcaaaagc ttatccagga gttgttgcag ttctaacagc
     8401 taaagatatt ccaggaacta taaaggtagg gcacttaaag aaggattggg atactttaat
     8461 accagaaggt gaaataacta gatacttagg agatggaata gttttagtag cagcagaaac
     8521 tagagaagcg ttagaagagg ccaaaaagct agttaagata gattatgagg aattaacacc
     8581 attaagtact acagcagatg cacttagaga agatgctcca aagatacatg agacagggaa
     8641 tatacttgtt aaggaacatt tagtaagagg aaacgctaaa gaaaagatag aaaattcaaa
     8701 atatgttgta actaaaagat atactacacc tgctacagaa cacgcatttt tagagcctga
     8761 atgtgcagta gctggtcctt atgaagatga tggagtaata atctattcaa cagaccaagg
     8821 ggtgtttgcc actcaacatg aatgtgcaga tatgttaggg ttaccatatg agaaggtaag
     8881 agttataaac aaaatggtag gaggagggtt tggtggaaaa gaagacatga gtgttcaaca
     8941 ccatgcagcc ttacttgcat accatacaaa gagatttgta aaagtacagt taacaagaaa
     9001 agaaagtatt attgttcatc caaaacgaca tgccacagaa atggaattta cgacagcatg
     9061 tgatgaaaat ggatacttaa cgggtatgaa ggcaacgata atatctgaca caggtgcata
     9121 tgcctctctt ggaggaccag tgctacaaag actgtgtact catgcagcag gaccatacaa
     9181 ttatcaagat attgatataa caggaacagc agtatatact aataaccctc ctggaggagc
     9241 atttagagga tttggagtta cacagagttg ttttgcaact gaatgtaatc taaatttact
     9301 tgctgaaatg gttggaatat ctccttggga gataagatat aaaaatgcaa taagacctgg
     9361 gcaagtttta ccaaatggtc aatttgcaga tgaaggaaca gcaatggcag agacactaga
     9421 agctgtaaaa gaagagtatg aaaagcatcc acgtgcaggt atagcttgtg ctatgaaaaa
     9481 tagtggggtt ggagttggta tacctgatgt tggaagatgt agattggtga taaaagatgg
     9541 caaagttcac ataagaacaa gtgcttcttg tataggtcaa gggcttggta ctgttatgac
     9601 tcaagtagtt tgtgaaactg ctagcctact tcctgaaaat atagtgcatg aagttccaga
     9661 tactcattta acaccagact ctggtaatac tacggcttca agacagacag tatttacggg
     9721 agaagctact aaggttgcat caaataagtt aaaagaagct ttagactctg gcaaaacttt
     9781 acttgattta gagggagaag agttttatgg agaatatgaa agtattacag ataaaatggg
     9841 ttgtgacaaa gaaaatccag taagtcatgt tgcttatgga tatgcaacac aagttgtaat
     9901 tctggatgaa aatggaaaac tagaaaaagt agtagcagca catgatgttg gaagagctat
     9961 aaatccaatc agtttagaag gtcaaataga aggtggagta gttatgggtc ttggatatgc
    10021 tctaaccgaa gattacccag tagtcaactc tgttcctaca gctaagtttg gtacattagg
    10081 tttatttaaa tctacagata taccagaaat acaagcttta atagttgaaa agaacaaaga
    10141 tttattggct tatggggcaa aaggtgtagg agaaatttgt acagttccta ctgcaccagc
    10201 agtacaaaat gcctattatg tatttgataa agaatttaga actgagctac cactacaaaa
    10261 tactccatat agtaaaaaaa agtaacaaaa aaaagaaata ttaaataaaa aaatagatga
    10321 aattgtaaat aaaaaaaacc cttttgcata aggtatattt taaatgatag aatatacata
    10381 tacaggaggg tttttctttt ataaaataag tttaataaaa ttgagaggtg agcagaatga
    10441 attttaataa tagtctagaa ttaactcagt ctcagaagct aataatgact acacaactta
    10501 agcaatcatt atcaatatta aatatgagta agttagagtt agaagaagag ataaaaagag
    10561 aagcagagaa taacccatta gttgaagtcg agaaaagtgg ggagataaat tgggaagagt
    10621 atataaaaga catggatagg tcaagacgtt tagatagaac agagataagc tataatcctg
    10681 ataatgaagt taatctagaa aatttagtta aatattcgtc aaatttatat gaagatttaa
    10741 aatttcagat aagcctgtat aaacttacag acaaagaatt ggaagtctgt gagtatataa
    10801 ttgatagttt agatgaggat ggatatttaa gaactgagga aaaagtaatt gttgatacct
    10861 taaagataga tgaagaactt tttgaaaaat gtttaattag tgttcagcag ttggaaccta
    10921 gtggagtagg ggcaagaagt ctttcagaat gtctgatgat acaaatggct agtttaggaa
    10981 tatataatga aattttagag gaaatcgtta caaaagactt aaatttaatt gcaaataata
    11041 aatacaaaga gataagtaaa aagtacaata tgtcattaca aaaatgtgtt gagctaataa
    11101 acataattaa gacattagac cccaaaccag gaagaacttg ttcagtagag aaaagtgtat
    11161 acattcaacc tgatgtaaca gtagaaaaaa ttgatgatga atttattgta tatttaaatg
    11221 agaaagattc ttatcaaata agaatcaata gttattataa agacatatta aaaaattcac
    11281 agtctgatga aagtgcaaaa gaatttataa aagaaagact aaattcagca acaggactta
    11341 tgaaaaatat agagagtagg aaaactacag tacttaagat tgcagaagag atagtaaaag
    11401 ctcaagatga atttttgaga aaagggacaa aatatataaa acctttgaaa atgaaagaca
    11461 tagcagaaaa attagaattt catgaatcta ctataagtag aggtgtaaat ggtaaatata
    11521 tgctaacacc ttttggcgta tatgaattta ggtacttttt cagtagcgcc attgaaactg
    11581 agaataatga aatggcatct agtacaagca taaaaaaaat tataaaggaa acaataaaag
    11641 acgaaaataa aaagaaacct ttaagtgatg accatatatc taagttatta aaagaaaaag
    11701 gaatcaatgt tgcaagaagg acagttgcca aatatcgtga agaattagga atcccatctt
    11761 caagtaagag aaaagaatac taataagtaa aagacaaata tcagtatggt aaggggaaaa
    11821 ggctaattaa gagatgaatg tagattgggc taaaaatttt ataaagtaaa ataatagaat
    11881 atggaaaagt attaaaatgc tataaggtat taaaaaactg gggaacatta aaacattaag
    11941 atagcaaaga acataaaaaa ctaaagaaga ttaaaacatt aaagaacatt agagctttga
    12001 ggaatattag aactttaaag aatattaaaa tttataaaat ataaaaatac taaaaagaat
    12061 tgaaaaactg gggaacatta aaatattgaa aaatgctaaa aaactagaaa acataattaa
    12121 ataagtgctt ataaagaatg attaaattaa ataagtgttt gtaaagaata attagatatt
    12181 agaaaaagaa gaatacctag ataaataaac tattattaaa tttaataatc acaaaaggaa
    12241 aacacgtagg gtataaataa aaatcttaac tagataaaag taaacttagt tgtagataaa
    12301 aataactaga aataaaagta actagaaata aaattattta tagtaataaa tattagaatg
    12361 aaaatataaa aaaatatata taaaaagcct tatataaaaa gaaaaaatac tgtgatttga
    12421 aaatggtgga gaaatatttt ataaaataaa aaaataaaaa agtttttgaa aaactattga
    12481 aatctatacg caaatcctat aaaataaaaa tatcttgtgg gacttaaata aacactaggg
    12541 acaaaaaaag tcccgaaagg aaagcaataa ggagtatgtt atgaagaact tattagaaat
    12601 tcaaaagaaa ttgattccac aggcaattga acttatggaa agaagatact ctattttaag
    12661 acaaatatct ttaagtgaac caataggtag gagaacatta tcaaatgtat tagagattag
    12721 tgaaagaata atcaggtcag aaactgagtt tttaaaagaa caaggactta tagacgtagc
    12781 tgtatctggg atgactatca ctaaagaagg ttctgatttg ttagataaac ttaaggacat
    12841 catgagtgac atcatgggat tgtctaagtt acaagataga gtcagagaaa agcttggaat
    12901 aaaaagggta atattagttc ctggaagctg tgatgaaaat ggaagtttat taaaagatgt
    12961 aactagatat ggatgcgaat atttcttagg cattttaaaa gatggagata tcgtttcaat
    13021 aacaggagga agcactatgt tggagttttc aaacgttatt aaaactgata agagatatcc
    13081 aaactctaca atagttccag ctagaggaag tatgggaact gatgtagaga tgcaatctaa
    13141 caatatagtt gcttcaataa gtaagaaatt acattctaat tgcaaacttt tacatatacc
    13201 agatgagttg gatgaaagtg caatgaaaac tcttagccaa gtgccagaga ttaagacaac
    13261 tttggagtat attaaaagaa cagacatttt agtttttagt ataggtagag cagatgtcat
    13321 ggcaaagaga agaaaacttc cagaagacaa ggttgatgag atattatcta aaaatgcagt
    13381 tggagaagca tttggatatt atttcaacaa agatggagaa atagtttaca aactgaacac
    13441 tgttggaatt gatttagaaa cagttaaaca agtaaaagaa actattgcaa tctttgcagg
    13501 gactgaaaaa gtagaagcct tattagctat atcaaatatg cataaaaaca tggttcttgt
    13561 aacagatgaa gaaagtgctt ataagatatt gtcattatta taaaagtcaa gacctagcac
    13621 agagctagaa aacatacatt tactttatag gaggtagcga aaatggttaa agtagctata
    13681 aatggatttg gaagaatagg aagattagca ttaagaaaaa tgatggagca acaagataag
    13741 ttcgaagttg ttgcaataaa cgacttaaca gacgctaaaa tgttagctca tttatttaaa
    13801 tatgatacag ctcaaggaag atttaacgga gaaatagaag ttaaagaagg tgcttttgta
    13861 gtaaacggaa aagaaataaa agtaacagca gaaagaaatc ctgctgacct accatgggct
    13921 gaattaggag tagatatagt attagaatgt acaggattct ttacatctaa agataaagct
    13981 gaagctcata tacaagcagg ggctaagaaa gttgttatat ctgcaccagc tactggagat
    14041 ttaaaaacaa tagtattcaa cacaaacagt gacatattag atggttctga aacagttata
    14101 tcaggagctt cttgtactac taactgttta gctccaatgg ctaaagtatt aaatgataaa
    14161 tacggtatag aaaaaggatt aatgactact atacatgcat acactaatga ccaaaatact
    14221 ttagatggtc ctcatccaaa aggtgattta agaagagcta gagctgctgc tggaaacata
    14281 gttccaaaca ctacaggagc tgcaaaagca ataggcttag taataccttc attaaaagga
    14341 aaattagatg gggcagcaca aagagtacca gttgtaactg gttcaataac tgaattagtt
    14401 tgtacattag gaaaaaatgt tacagttgaa gaaataaatg cagctatgaa agaagcttca
    14461 aacgaatctt tcggatatac tgaagaaatg ttagtatctt ctgacataat cggaataagc
    14521 tacggttcat tatttgacgc tactcaaaca aaagttatgg aagtagatgg aaaacaatta
    14581 gttaaagttg tttcttggta tgataatgaa atgtcttata cttctcaatt aataagaact
    14641 ttaggatact ttgcacaatt agcaaaatag tcagttttga gaaaatagaa aagtagattg
    14701 acaaagtccg agtttgtagt ttaatactac ggactcagga ctttttttaa gaaaaaatat
    14761 ataaaataca cctaaataat gttacatact atatataaaa atatgataat atgatatatt
    14821 taatagggta aaagtatgta ctatattata cttggcaata aaaaattact ttattaaaag
    14881 gagagatatt tatgtcaatg cttaacaaaa aaactataga agatattgat gtgtgcggaa
    14941 aaaaagtatt agtaagatgt gactttaatg tgccattaca agatggtgta attactgatg
    15001 aaaatagatt gaacggagct ttaccaacta ttcaatattt aatttctaaa ggggcaaaag
    15061 ttatattatg ctctcactta ggaaagccaa aaggagaagc taaaccagaa ttatctttag
    15121 caccagtagc aaagagatta tctgagatgt taggaaaaga agttgtattt gcagcagacg
    15181 ataatgttgt aggagaaaat gcaaagaaag caactgaaaa aatggaaaac ggagatgtag
    15241 tattattaga aaatactaga tatagaaaag aagaaactaa aaacgaagag aatttctcta
    15301 aagaactagc atcacttgct gaaatatttg taaatgatgc atttggaact gctcatagag
    15361 cacattgctc aacagttggt gcaggcgagt ttttacaaga gagagtttgt ggatacttaa
    15421 ttcaaaaaga attaaaattc ttaggagaag ctgttgcaaa ccctgtaaga ccatttacag
    15481 ctatacttgg aggagctaaa gtttctgata aattagctgt tataaacgaa ttattagaaa
    15541 aagttgataa tctaataata ggtggaggaa tggcttatac attcttaaaa gctcagggtt
    15601 atgaagtagg aacttcttta cttgaaatag ataaagttga atatgctaaa gaaatgatgg
    15661 aaaaagctaa gaataagggt gttaacttat tattaccagt agacgttgtt atggcagacc
    15721 actttgcacc agatgctact ccaatagtaa cagaagatgc taatgtaaaa gaagattaca
    15781 tgggactaga tatgggacct aagactatag caaactttgt aaaaactata aaagaatcaa
    15841 aaactgtagt atggaatggg ccaatgggag tatttgaatt tgaaaacttt gcaaatggta
    15901 cattatcagt agctagagct atggcagagt tgactgatgc aactactgta attggcggtg
    15961 gagatagtgc agcagcagtt aatcaattag gatttggtga caagatgact cacgtatcaa
    16021 caggtggagg agcatcacta gaatttttag aaggtaaaga attaccaggt attgcagcat
    16081 tagataataa ataaactaat atatagagga gattgtaaaa tgagaaaacc tataattgca
    16141 ggaaactgga aaatgcataa aacaataaaa gaagctttag aatttgtaaa tgaagttaaa
    16201 gataaagtaa attctgataa agtagaagct gttatatgtg caccatttac tttattaaag
    16261 gatttaaaag aagctactaa gggtacaaat ataaagatag gtgctcaaaa tatgcacttt
    16321 gaagaaaaag gagcatttac aggagaagtt tcacctctta tgttaaaaga aatagatatg
    16381 gactatgttg taataggaca ttcagaaaga agacaatact tcaatgaaac tgatgaaact
    16441 gtaaacaaga aagttttaaa agcattagaa gtaggaatag acccaatatt atgtgttgga
    16501 gaaactttag aacaaagaga agctggaaaa actaaagatg tttgtaaagt tcaagttgaa
    16561 aaagctttag aaaatgttct taaagatgat ttagcaaaag tagttgttgc atatgagcca
    16621 atctgggcta ttggaactgg taaaactgca actgctgaag atgctaatga cgttatatct
    16681 tatataagag aagttataaa aggattatat ggagaattag ctaatgaagt tagaatacaa
    16741 tacggtggaa gtgttaaacc ttcaaatgta gccgaaataa tgggtcaaag tgatatagat
    16801 ggagctttag taggtggagc tagtttagct agtaatgatt acctagacct tgttaatttc
    16861 taaggagtga aatgatgaaa aaaccagttg ctttaattat tatggacggg tttggataca
    16921 ataaggatgt taagggaaat gcaattgctg agtccaaaac tcctaattta gatagaataa
    16981 aaaaagagta tccaaacaca ttgataaatg ctagtggtct tgatgttggt ctacctgatg
    17041 gtcaaatggg aaattcagaa gttggacaca ctaacatagg tgcaggtaga atagtatatc
    17101 aagatttaac tagaataaca aaatctataa aagatggtga cttttttact aataaagttt
    17161 tatgtgaagc aatggataat gcaaaagaaa actctcttca tgtaatggga cttttgtctg
    17221 atggaggagt acattcacat atagaccatc ttaaggctat aatcaaaatg gcaaaagata
    17281 aaggcgtaca aaaggtgtat gttcacgcat ttactgatgg tagagatact gaccctcaaa
    17341 gtgcattaga atatgccaaa gaagtacaag ctagtatgga tgaaattgga gtaggtgaat
    17401 ttgctacagt ttctggtaga tactacgcta tggatagaga taagagatgg gaaagagtag
    17461 aacttgcata caatgccatg gttagaggta ttggtgaaaa agcaaattct attgaggaag
    17521 ctattcaaaa ctcttatgat gatggaaaaa atgatgagtt tataatgcca acagttatta
    17581 tgaaagatga taaaccagtt ggaagtataa aggaaaatga ttcaattata ttcttcaact
    17641 ttagaccaga tagagctaga caaataacta gagctttagt ttgtgaagag tttgatggat
    17701 ttaaaagaga agatattaaa aacttctttg tatgcttaac agagtacgat ataacaatag
    17761 agaatgtgca tatagcattt ggacctcaat ctttagccaa cactctaggt gagtatctag
    17821 ctaaaaatgg aaaaactcaa cttagagctg ctgaaactga aaagtatgca catgtaactt
    17881 tcttcttcaa tggtggagta gaagagccaa ataaaggtga agaaagacta ttgatacctt
    17941 ctccaaaggt tgcaacttat gatttaaaac cagaaatgtc agcatatgaa ttgacagata
    18001 aagctttaga taaattagga gaagataagt ttgactttat agtattaaac tttgcaaatc
    18061 ctgatatggt aggtcacaca ggaagtatag aagcggctat aaaggctgtt gaaactgtag
    18121 atacttgtgt aggaaaattg atagataaaa tagtagagtt aggtggaagt gcaataataa
    18181 ctgctgacca cggaaatgct gaatatatgt tagacccaga aacaggaaaa actgtaacgg
    18241 ctcactctat taatccagta ccatttattg tagttggtca agaatatgaa agtgcaaaat
    18301 tattggatgg aggaagatta tctgatatag caccgactgt tttagatatg atgaagttag
    18361 aaaagccaga agaaatgact ggccattcac ttatttcaaa atagtcgatt ctgataagta
    18421 gataataata tataaactac aatataaatt taaaaataat gcagtttagg tttattttgt
    18481 aaagtttaga ctttatgaca taagatttaa attttgtaag ttctggatat tgtaaggtgt
    18541 agatgaagat atattttata aaatttacaa gatagacaat ttataaaata aaaaattaca
    18601 atatagataa taatgtaaat aatgatataa ataataacaa gatagacaat aacaatataa
    18661 ataataataa ttaa
//
LOCUS       DAEMHZ010000071        18611 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000071 DAEMHZ010000000
VERSION     DAEMHZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.071"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1081
                     /locus_tag="KPK72_002299"
     CDS             <1..1081
                     /locus_tag="KPK72_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489508.1"
                     /translation="IDDLKDIENEIVGYTLKHAPFFIQEDFDSLTILKSFQKEIISIK
                     NSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKLMQDVFIYAKQPLLK
                     ENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELNDEIRIEVINSLYKQI
                     QEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNLLSDLSYNISSYLNE
                     VEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRLNHLVENMYPYYVDE
                     TSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLNNSTKERRKDIIEKI
                     YNSVDYGTNKLDENMFFTGGIKYVK"
     gene            1071..1391
                     /locus_tag="KPK72_002300"
     CDS             1071..1391
                     /locus_tag="KPK72_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489509.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            1589..2458
                     /gene="galU"
                     /locus_tag="KPK72_002301"
     CDS             1589..2458
                     /gene="galU"
                     /locus_tag="KPK72_002301"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2489510.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            2482..3495
                     /gene="galE"
                     /locus_tag="KPK72_002302"
     CDS             2482..3495
                     /gene="galE"
                     /locus_tag="KPK72_002302"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG2489511.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            3607..5541
                     /locus_tag="KPK72_002303"
     CDS             3607..5541
                     /locus_tag="KPK72_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG2489512.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            5696..6559
                     /locus_tag="KPK72_002304"
     CDS             5696..6559
                     /locus_tag="KPK72_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG2489513.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(6767..7648)
                     /locus_tag="KPK72_002305"
     CDS             complement(6767..7648)
                     /locus_tag="KPK72_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2489514.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            8061..8909
                     /locus_tag="KPK72_002306"
     CDS             8061..8909
                     /locus_tag="KPK72_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG2489515.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            8988..10910
                     /locus_tag="KPK72_002307"
     CDS             8988..10910
                     /locus_tag="KPK72_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2489516.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            10992..11855
                     /gene="aroE"
                     /locus_tag="KPK72_002308"
     CDS             10992..11855
                     /gene="aroE"
                     /locus_tag="KPK72_002308"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2489517.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKNVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            12055..13272
                     /locus_tag="KPK72_002309"
     CDS             12055..13272
                     /locus_tag="KPK72_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2489518.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MKYEKEKVYDYDV"
     gene            13508..15373
                     /locus_tag="KPK72_002310"
     CDS             13508..15373
                     /locus_tag="KPK72_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG2489519.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTVKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIYDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNIILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(15551..16915)
                     /locus_tag="KPK72_002311"
     CDS             complement(15551..16915)
                     /locus_tag="KPK72_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2489520.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(17094..18437)
                     /locus_tag="KPK72_002312"
     CDS             complement(17094..18437)
                     /locus_tag="KPK72_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2489521.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPRAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(18510..18611)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         7170 a         2197 c         3247 g         5997 t
ORIGIN      
        1 aatagatgac ttaaaagata tagaaaatga aatagtggga tatacattaa aacatgctcc
       61 attttttatt caagaagatt tcgatagctt gactattcta aagtcttttc aaaaagaaat
      121 catatctata aaaaatagtg atataaaaga cattgtaatt aataaagaac ttgaaaacga
      181 aaattctaat tttgtagaaa cggaaataaa atttaataat caaaaagtac ttttaaactt
      241 aagtaaaaat atggttcaaa aacttatgca agatgtattt atatatgcaa agcaacctct
      301 acttaaagaa aattctattg aagtgatata taagaattgg tctaaagcta tgaatgatat
      361 gataatattt aatttctttg gaaatattta ctatatgaaa tctgaatttg ataagatttt
      421 agaaaaagag ttaaatgatg aaataagaat agaagttata aattcacttt ataagcaaat
      481 acaagaacaa agaaacaatt tagatttatt gtcagcatat atgccaagaa tattagaaaa
      541 tcaagaaata gatttgtttg aaaagtataa tactaaactt gatgtacaag tgtttaagca
      601 gattaaaaat ctattgtctg acttatcata taatataagc agttatttaa atgaagttga
      661 aaaaagttta gacaatatca tctttgtaat ttcaggagaa gataagaaga aatacaatta
      721 tagaatgtta aaagaaagtg aaagtgcaag tcttgatgta tttttaaaac aagccatatc
      781 aagattaaac catttagtag aaaatatgta tccttattat gtagatgaaa ctagtaggga
      841 aatgtttaag ttatttgagt tattgtggaa aaactataga aatattgatg atgatagtat
      901 aaaagaaata ttattcgaga gaataacaaa tacatatgta tttaaacaat tgacattaaa
      961 taatagtaca aaagaaagaa gaaaagacat aattgaaaaa atatataact ctgttgatta
     1021 tggaaccaat aaactagatg aaaatatgtt tttcacagga ggaataaagt atgtcaagta
     1081 aatacaatgt ttggactttt aactatgagt tcttaggatt ggactcaggg gatgaaaaaa
     1141 ataatgaagt ttattacgag attgatttag atgaaatttt aaatgaaaac tatatagatg
     1201 aagatataga tagtggttta gattctgatg gaaatagtat tttaggagaa ttaatatcag
     1261 aaatggatat agatgaggat gtcagtgttg atatgacata tcaagaacta gaagttgact
     1321 tagaagattt agattcttat ctagatgaag atatagatag tgatataaaa ggcatcttag
     1381 atgaaattta gtacctgtat aagttttgga taaaatataa acaatacaat attaacttat
     1441 aaaactaaag gtagatgaaa tttcatctac cttatattat cttatagcat taaatattgg
     1501 gataatatag agtttgaaga aaaagtataa atatggtata ttaatttggt acaatagaaa
     1561 atttacgaag aagggtgaaa aaatgcagat gacagttaaa aaagcaataa taccagcagc
     1621 agggctagga actagatttt taccagcaac aaaatcacag cccaaagaaa tgcttcctat
     1681 agtagataaa ccaactttac agtatataat agaagaagct atagaatcgg gaattgaaga
     1741 aattcttata ataacaggta gaaataaaaa atctatagaa gatcattttg ataaatcagt
     1801 tgaattagag ttagaactag agcaaaaagg taagacagag atgttagaaa tggtaagaga
     1861 catatcaaat atggtaaata ttcattatat aagacaaaaa gagccaaaag gtcttggaca
     1921 tgcaatttac tgtgcaaaaa gttttatagg tgatgaacct tttgccgtat tattaggtga
     1981 tgatatagta gattcagatg ttccgtgctt aaaacaatta atagatactt ataatgagta
     2041 taaaacaact gtattaggtg ttcaaaagat agctaaagaa gatactaata aatatggtat
     2101 attggatgta aaacatatcg aagatagagt atataaagtt aaagatatgg ttgaaaaacc
     2161 tgcaattgaa gaagctccat ctgatatagc tatacttgga agatatataa taactcctgc
     2221 aatatttagc atattagaga aacagacacc tggtaaaggt ggagagatac aactgacaga
     2281 tgcacttcaa actcttggaa aacaagaggc tatttatgct tataactttg aaggtagaag
     2341 atatgatgtg ggagataaga ttggtttttt agaggcaaca atagactttg ctctaaagag
     2401 agaaaattta aaagatgatt taatggacta tatgagaaaa aaagtaagtg aaaaataaat
     2461 attagaaaag aggtaattac aatggcagtt ttagtagctg gaggagcagg atatatagga
     2521 agccatacag caatagaact tttagaatca ggttatgaag ttgttatagt tgataactta
     2581 agtaatagca actcaatagt ggttgataga atcaaggaat tatctaaaaa accagtaaaa
     2641 ttttataata ttgatatcag aaataaagat gaaatgcatg ttgtatttaa agaaaataat
     2701 attgaatcaa ttatacactt tgcagcttta aaagcagttg gtgaatcagt agaaaaacca
     2761 attgaatatt acagcaataa tctaataagt accttaaatt tatttgaatt aatgagagaa
     2821 tatggtgtta aaaagtttgt atttagttct tcagctactg tatatggaga cccacatact
     2881 tgtcctatat tagaggattt cccactttct gtaaccaatc catatggaag aactaaactt
     2941 atgatagagc agatgttagt agatatatca aaagcagata agtcattgga tatagctctt
     3001 ttaagatact ttaatccagt cggtgctcat aaaagtggta gaataggtga agaaccaaat
     3061 ggagttccga gcaatctaat gccttatata acaaaaatag cagtaggtaa attgaaggaa
     3121 ttgagtgttt atggtaatga ctatccaaca catgatggaa ctggagttag agactatata
     3181 catgtattag atttagctgc tggtcatgta aaagcattac aaaaattaga agaaaatcca
     3241 ggacttgttg tttataattt aggaactgga aaggggtata gtgttcttga tttagtaaaa
     3301 gcattttcta aagcaagtgg aaaagaaata ccatataaga ttgtaggaag aagggctgga
     3361 gatgtagcca tgtgttatgc agattcttca aaggcagaaa aagagcttgg atggaaagct
     3421 aagtatgaac ttgaagagat gtgtgaagat tcatggagat ggcaaagtat gaatcctaat
     3481 gggtatgaag aatagaatat atacttatat gtataaatgt aacaaatttg taaatataag
     3541 gtattggtaa ttttatgtta taattagaca catgtagtaa tgtttctaaa agagggggaa
     3601 gttatagtga aaaaaagtct aaaaaagtat ttagtactag cactgacttt agttttagta
     3661 tttgcatgtg gcgtaccaga atcaagcgca gccagtaaac atgtgattat agtaaattca
     3721 agaaaaaata ctctaggata ttttgtaaat aataaacttg taaaagaatt tagagttgca
     3781 acaggtaaaa aggggtcaga aacaccaaca gggaaaacaa aagtagttaa caagataaaa
     3841 aatagaccat attataaagg taatatacct ggaggaagtc caagaaatcc attaggagat
     3901 agatggatgg gattagcatt aaaaggaact tatggagata catatggaat acatggaaat
     3961 aataatgaga gttctatagg aaagcatatt tctggtggat gtataagaat gcacaataaa
     4021 gatgttagat ggttatttga ccaagttcct gtaggttcag atgtaattat agattatagt
     4081 aatgacagct atgtaaagat agcggcaaaa tataagataa atctaaatca aacaggatgg
     4141 aaaacagaaa atggtaagaa gtattatgta aaatctgatg gaacatacca aaagaatagt
     4201 tggctaaaag taaatggaaa aatgtattac tttgatgctt caggagtaat gcaaacagga
     4261 tggaaaacaa ttaataacaa aaaatattat ttaggaactg atggagcaag agtatctgga
     4321 tggaaagtaa tagacggaaa aacttattac tttaactcag atggagtaat gcaaacagga
     4381 tggcaagaaa agaatggaaa gaaatactat ctaggatctg atggtttagc agtaacagga
     4441 tggcaagaaa tagatggtaa taagtattac tttgacaaaa caggaataat gcagacagga
     4501 tggcaacaaa tagatggtaa gtcttactat ttagacaaag atggaaagat gctaactggt
     4561 tctcaaaaaa tagatgggaa agattatact tttaatgaag atggaactat aaatcctaca
     4621 tgggatacaa ttataggtgc aaatagattt gatacagcta aaaagataag ttcagttgga
     4681 aactggaatg ctgatagttc agatactgta atattagtaa atggtaatgc aatagctgat
     4741 ggtataacag caactccttt agcaagtagc tatgactcaa caatactact tactaataca
     4801 gctaatttac caactgaaac tgttgaagaa atgaagttat tagcacctaa aacagtaatc
     4861 ttgataggtg gagaaaatgc aataagttct aaattagagc aagaaataaa aactactttc
     4921 aacacagaaa caaagagaat agcaggtcaa gatagatatc aaactgctac aagaatagct
     4981 gaagaacttg gaaatcgaga agaaataaaa actgcatata tggtttctgg aaatggggaa
     5041 gcagatgctt tatctgtagc ttctaaagct ggggaagaaa aacaacctat aatattagtc
     5101 aataaagatg gaattacaga agaatcttat aaatggctaa cagaaagaaa attagaaaat
     5161 gcatacttta taggtggacc aagtgcaatt aacgattcag taatagcaaa aatgaatgat
     5221 ataactactg aagatatttc aggaaataga atatatggag atagtagagt agatactaat
     5281 gccaaagtaa tagaaaaatt ctatggagat actgatttac aggctgtatt agttagtaaa
     5341 tcagatgctt tagtagatgc tttatcagct ggaccattag ctgtaaaatt acattcacca
     5401 atagttttaa tggataattc aggattatca tcagagcaac aaagagtatt tgcaaataaa
     5461 aaagttgaaa ctccttatca aataggaggc ggagtatctt atatagtaat ggataaatta
     5521 atggatattc ttgcaaaata gtaaaaatat atattgaaaa ataattataa ttgagatgtg
     5581 aaattcaatc aataaaagct tgattgtttt acatctcaat tttttagtat aaaattaagt
     5641 ttaagttata taaaaatctt ggaatatttt taaaactcat ataaaatgaa tatagatgct
     5701 atcatcatat gttaagaggg tgagtaagat tataaagaaa aaatatataa tgtttattga
     5761 tgaaagtgga agagctgata taaatcatga agacccattt acattgacag gagtaatttt
     5821 tgaatataaa tatgcagtaa gtcaaggcga ttatgtatgt tctttgagga aagaaatgga
     5881 tgcatttaaa gagtattgtt ttggaaccag taacatagga atacatttga cagatatatc
     5941 aagaaaatca agagcatttg agaagtttga tgattcacag attaagttat tctatgatga
     6001 gttgccttta tttctatcta gattagagtg tactataata tcagtaacag tagataaagc
     6061 tagattaaag aaatattatg caccttcaaa ggaaccgtat gttgtggctt ttatacatgt
     6121 tcttcagaat ttttattcat ttgtaaataa tgaaactgta gaatctgcac gaatagttat
     6181 agagtcaagg gatgatgtct caaacttaaa agtacaaaag gcgttttttg atgtatttaa
     6241 taatggaaca atatacttag atatagatga agaattaaga gataaggtta agggttttat
     6301 aattgccaaa aaagaagatg ctgactatag agaaggttta gaaatagcag atattttatg
     6361 taatcctctt agcagagcaa gaagagggtt aatagaagct aatccaaagt gtatgcaata
     6421 tggagaaaaa aacaaaatat tcaaatctgt cagaaacaaa atatatactc caacgagtgt
     6481 gaatgatata agaaattggg gatttaaaga agttcctatt gtagagcctg ttggaccatg
     6541 gcttgaaaaa atgcgttaga tacaattgac aaattttaaa aaatctgata taatgtaagt
     6601 aacctagtta ttcaggaact gaatccagtt gctgttgtat aacgataatt actgagactc
     6661 ggatgccgac tgtcagttag gaatctatac ttttaaaagt atagattttt ttattttata
     6721 aaaagaataa aagaggatat ttaaaaataa cccctttatt aataaattat aattgagcgt
     6781 ttttaatgac aaagttttta aagtttttaa ttgcaggagg caaatatcta tcttttataa
     6841 atgctaagta aaagtttctt tcccaacttg ggtgaataat ctgaattact tttacttttg
     6901 taaagctaag catattcata tttgggacta cagcaattcc gaaatttttt gcaactaatc
     6961 cagcaattac ttggtcttcc tctacttcat agactatatt aggctgttga tttatttttt
     7021 taaacatatc atcaattata tatctaagac cgcttctctg attaaatact atttgaggat
     7081 aaggtatggt ttcttttaaa tctatagtat cctttgcagc tagtggatga ctatatggta
     7141 caattagtac taaatcttgt ttggcaacag gtataaattc aattcccttt tctttttcaa
     7201 gtttcgaaca aaatgcaata tcatattttc tttctttaag accttgaata atgtcagtag
     7261 ttacaccagt atgaaattta aaatctattg atttagcagg attagatttt aaaaatttgt
     7321 gcactatgtc tgggataaaa tcagtaccaa gtgttctaag aaaagctaag tctatttgtc
     7381 cttcaccagt accagttatt tttaatgatt ttacacttga atctaaaatt tctaatgatt
     7441 tttccacatc ttttaaaaat atttttccat acttagtaag gactatattt ctaccttctt
     7501 tttcaaacag agccaccccg agttcatttt ctagtaaaga aatagcatga cttaagcttg
     7561 gttgtgttat agataaattt tctgccgctt ttgtatagtg ttctaaatgt gccaatgtaa
     7621 caaaatagcg taagtgatat aaattcaatt tttgttcatc ctttcatagg tatttttatt
     7681 tatatatttg tagaagttaa cctttaagat atttttacat ttttattata tatgattata
     7741 gagtaagaat aaaagctttt aaaaaaattt atcgtttttt gtctgaatta taagataaat
     7801 atatttatac gacctaatag aaaaatacaa gaattatctt ataaataaaa tctataaaca
     7861 aaatctatga attaatagga aattatcaat ttgttattag taaagtctcg tgatacaata
     7921 aatttataaa atctagataa atagaaatca aaaaatactt ttatttttaa ggtaaaataa
     7981 tactactagt aggataaata aaaatataaa atcaaaaata ctattaatta taaagaaaat
     8041 aaagggggca aatttaagct atgaataatc aaaaaaatct taatattcca attacaatta
     8101 gttcttatac attgggaaca gaagtttcat tccgtgatag agttcgtatt gcaaaagaag
     8161 ctgggtttga tggtattgga ttgcgtgctg aaaattatat tgatgcaaaa aatgcaggtg
     8221 ttacagatga agaaatgatt gcaattctta atgaatatga tatgaaagtt acagaagtag
     8281 aatatataac tcaatgggga agagaagaag atagaacgga agctcaaaga gaaaaagagc
     8341 aaaatgttta tcatatggct cacttattta atgttaaaca tataaattgt ggattattag
     8401 agatgatacc agaagaacaa attataactg cattgggaga attatgtgac agagcaggtg
     8461 aacttattat aggtcttgag tttatgccat atagtggtgt accagatttg gctactgcat
     8521 ggcgtgttgt gaaaggatgt aatcgtgaaa atgcaatgct tatattagat acttggcact
     8581 gggctagagc aaagcagaca gcagaggacc ttgcacctgt tccagcagat aagatagtat
     8641 caattcaagt gtgtgatgtt ttagaaactc cttatgaaaa acttagggat gaatcactac
     8701 atgaccgttt ggctccaggt gaaggatatg gaaacacaga ggaatttgtg aaaataataa
     8761 aagaacacaa tatatcacca agagtcatag gggcagaagt aatatctgat tctctaatat
     8821 ctaaagggtt atccactgct gctgaaactg tatttaatgc aactaaaaaa gttttggata
     8881 aagcatggtc tgaagtatct ccaaaataaa cttatataat ataaaaatat acagagtatt
     8941 ataatataaa acttttaaat ataaaacttg aaaggaagga aaaccttatg aagattagtt
     9001 ctatgtttac acctataagg attggttcaa tgacagttcc aaatcgtttt gtagttcccc
     9061 ctatgggaaa caattttgca aacacagatg gtactttaag tgaaacttct aaggcttatt
     9121 atcttgagcg tgctttaggt ggttttggat taataactat agaatctagt gttgtagaca
     9181 aaaaagcaaa aggaggtcct cgtaagcctt gtttatatga tgatagcaca atagatagtt
     9241 ttaaaaatgt aatagatgct tgtcatgatg caggttcgaa ggtatctata caattacaac
     9301 atgctggttc tgaaggaaat gaaaaagtag caggacatcc attaaaagct gcttctgcaa
     9361 tacctgcatc aaatggtcgt aatacaccac tagcaataac aacagaagaa atatatgaac
     9421 ttatagaatc ttatggagat gctgcattac gtgcacaaaa agctggtgca gatgctgttg
     9481 aagttcactg cgctcatgga tatctggtta gtagttttat atcacaaaga acaaataagc
     9541 gtgtagatga gtttggtgga tgttttgaaa atagaatgag attacctcgt cttatcattg
     9601 aaaatatacg taaaaaagta ggaaattctc tagcaatatt atgtcgtata aacagcactg
     9661 atgatgttcc aggaggaatt gatgtacacg atagttcagt aatagcagca tatctagaag
     9721 attgtggaat agatggactt catgtttcac gtagtataca tatacatgat gaatatatgt
     9781 gggctcctac gactttacat gctggattca gtgcagaatt agttactgaa ataaaaaaag
     9841 ctgttagtat accagtaata acagttggac gttatacaga gccacagttt gctgagttaa
     9901 tggtcagaca aggaagatgt gatttagtag cattcggtag acaaagcttg gctgacccag
     9961 aaatgcctaa taaagctaaa aatggaaaac tagacgaaat gataccatgt attgcatgct
    10021 tacaaggatg tgtaccaaat atgttccaag gcaaacctat agcctgtctt gcaaatccta
    10081 tattaggaca tgaagcagag cttaagccag ctgaaattag caaagaagtt cttgttgttg
    10141 gtggaggtgt tggaggtatg cttgcagcat gggtatgtgc taaaagagga cataatgtaa
    10201 ctttagttga aaaatctgaa gttcttggtg ggcaaatgcg tttagctgca tatcctccag
    10261 ggaaaggaga tattactaac ttagttagaa gttatatttc aaaatgtaat caatatggtg
    10321 taaaaatatg tactaataca gaagctacag tagaacttat aaaggagaaa tcacctgatg
    10381 tagttattat agctactgga gcaactccac ttgtacttcc tataccagga atcaatgatt
    10441 caggattaat tcatgctgta gaccttcttg atggtaaaaa atcttgtggg aaaaaagtac
    10501 ttgttgttgg aggtggaatg gttggatgtg aagtggcagc cttccttgga gaacaagaac
    10561 atgaggttac tgttattgag cttagagaag aagttggtgc agatgtaatt tcagaacacc
    10621 gtaaattcct tatgaatgat tttgaaaatt ataatataca taccattact ggtgcaaaag
    10681 tttctaaatt ttatgatgat ggtgtcgact atttacttac agatggcaca gagcatgatt
    10741 taaaaggttt tgattcagta gtacttgcta tgggttctcg taattacgac ccattaagtg
    10801 aagttataaa agaagttgta aaagaaactt atattgttgg tgatgcagta aaagctcgta
    10861 gggcattaga tgcaacaaaa gaagcttttg aagtagcatt aaatatataa ataataaata
    10921 aatttttctt tgtaaataac tgaataggtt atttgcaaag aaaatgataa tattgggagg
    10981 agattatagt tatggaaatt tcaggtagaa caggattatt tgcattaata ggtacaccgg
    11041 ttggtcactc taaatcacca gtgatgtata attatagttt taagaaatta gatttagatt
    11101 atagatattt agcctttgat atcacagtag ataaggtaaa agaggcactt ttagcaataa
    11161 aaacttttaa tataaaagga gccaatgtta ctatgccatg caaatctgct gttacagaat
    11221 atatggatga actttctcca gcagctcgta taattggagc atgtaataca attgtaaatg
    11281 acaatggaaa gttagttggt catataacag atggagtagg ctatgttcgt aatttaaaag
    11341 aaaatggggt agaagttaaa ggtaaaaaga ttacaattat gggtgctggt ggtgcagcaa
    11401 ctgctataca agtacaatgt gctttagatg gagcaagaga aatatctatc tttaatccta
    11461 aggatgattt ttataaaaga gcagagcaaa cagttgagaa tataaaaaaa aatgtaccag
    11521 aatgtgttgt taatctatat gatttagaag atacaaacaa actttatgag gagatagaat
    11581 caagtgatat attgacaaat gcaactctta ttggtatgaa gccttatgac aatgaaacaa
    11641 atataaaaga tactagtgtt ttaagaaaag atttagtagt aacagatgtc gtttataatc
    11701 ctaagaaaac aaaaatgatt gaagatgctg aagctaatgg atgtaaggct attggtggat
    11761 taggaatgtt attataccaa ggagcagaag cttttaatct atatactgga ttagaaatgc
    11821 ctgtagaaga agttaatgaa ttatgcttta aatagataag agtaatatct taaacaatga
    11881 aaaaacatac gtatttcaat atttttaata ataaattaca aattgtcata gtgggttttg
    11941 cctcatagtc cagttaaatt tgaagcttta actgactata ctttaaatcc attatgggga
    12001 tgagtttatt gtacacaaaa atgttaataa attaacaaga atgggagaaa aattatgaac
    12061 aataaaaaat attatcctac tgccatagcg ttgtattttt catatttttt acttggtatt
    12121 ggaatttcaa ttttaggaca atacaaacca gaattttcat caatgtgggg ggctaaaaca
    12181 ttatcagatg gaacattgga tgtaagtatt gttttagctg ttattgctgc actaggatta
    12241 gggagactta tatcttatcc atttgcagga cctatttcag ataagtatgg aagaaaagta
    12301 agtggtctta taggaaactt tctacatgca attttctttg ttggaattgt attttcacca
    12361 aatttttata tagcatatgt atttgcaata attggagggg cagcaaattc attcttagat
    12421 acctgtgtaa ctccttcttg tatggagata tttgctagtt taggaacaat agcaaatatg
    12481 tttactaaat ttacaatagc tttagcacaa tttttgcttc cttttataat tggatttgtt
    12541 gcagcaaatt caatatcatt taaagttatt tttatagtaa cagcgattct tattgtagta
    12601 gatgcctttt tgatagctgt gttaccattt ccaccagcta ataatgttat tgataacaaa
    12661 ggcaaaacag ttaaatcaga gaagatgaaa tttacaccaa caagtatagc tcttgtttgt
    12721 attggattta catgtacatc tacatttgta ttatggttaa attgtaatca ggaattgggt
    12781 aaattgtatg ggatggctga ccctacaaaa attcaatcct tttactcaat gggagtaata
    12841 tgtgcagtat taataacttc tttacttatt aaaaaatata ttaaaccaat aagaatatta
    12901 gttatatatc ctataatagc tttacttatg ctattagttg tatattttgt tcaaactcca
    12961 actatttgta tgttaggggg atttgtaata ggttattcag cagcaggagg tgttcttcag
    13021 ttaacaacgt caactgcaaa tgaaatgttt ccaactaata aaggaaaaat aacatctata
    13081 gttatgattg catctagtat agcaaactat gttattttaa atatagcagg tattattaca
    13141 aaatcagggg gagttaatgg accaaaatat gttgtattgt ttaatgtggc aataacattt
    13201 gttggaattt tacttgcatt gtttgtaaac atgaaatatg aaaaagaaaa agtgtatgat
    13261 tatgatgtct aatattagta taatatatgc atagagaagt ctaaatctag gatagatttt
    13321 tattaaaagt aattaaaatt aataaggata ttatttacag ttataagata gggtatttac
    13381 ataattaata ataaggatat tactagaatt tcaatttcta gtaatatttt tttgcgtata
    13441 aatgtaaact tgacgaaata agtagtataa tcaatataaa ttacatcatg taaaggagga
    13501 taatttggtg gagaatgttt ttaagcgatt gcaggaattt aatggttatg atggatacaa
    13561 agaatcattt gaaatgaatt acctatgtat atatgaaagt ataccgttaa gagaacaagt
    13621 agagcttgca aataatctag tagatgaaat tttaaatatg tataaaagtg aatctaatga
    13681 aatttattta ttggaggata gtaattctaa atctttaatt tgttatttcg aaatttttat
    13741 gaaaaagata aatactttgg taaaagaaat gattattgat gagaaatggt tatataaatt
    13801 aactaaagaa ttaatatata agagtaaaaa agttgagtat gtaaaactag ggttagtttt
    13861 aagtgagaaa tatctaaatg tagaaaactt aagagaagtt gtagatacat tttctaaatc
    13921 tggagaatac gtattttatc tgagtaatac tgttaaaaaa cttgaatttt acaatacata
    13981 tttatttaat ttatctaaaa aggctacagg ttcaataaaa gtatttgcta ttgttaatat
    14041 ggaaaattta gattctaaaa taaattccta tctaattgaa gatggttata aggatacaaa
    14101 gtacgaacga ttattgatga actatattat ttcaattgta gatttgaatg aatatcttga
    14161 aaaacgagat ttagataaag aaaagataaa taatttagca cgtcttatat gcaattactt
    14221 gctaagtgta gaatttaaat atataggtaa taaattagaa ttagtgaatc gatttttacc
    14281 tactgtagta aattatggga ctaactttga aagtttatat tcaattttcc taatagctat
    14341 aaacgtctta aaagatgaaa atatagagtg caataagatt gaatttgaaa aagaaataaa
    14401 tgatatcttg ctcagtgaaa aatggaaaaa tatatatttt gaagcattaa gagatgctag
    14461 tggaaaaaca gaagatatta taaaaatgtc agaaatttat gatgtgaact tatcttttga
    14521 tgacttgttg ccatatctga atagagatat tagagatttt gaggtatatt ggcatatatc
    14581 taaaaaggga acgacaagtt ctagattaaa attattaaat ttttttgaag agacgtttaa
    14641 aatatatgat ttaattggaa aaatgaaaga tatagaaaaa gataaattga ctcaagaata
    14701 ttatgatgat atgttatttt ttattgtgct taaaggaagt aaatcactat atcctgaagg
    14761 aaagaatata tctttaaaag gaatatttgg aaatataaat gaagtaagaa aagaatccat
    14821 aaatatctta aaaagataca gagagaaact atctcttgaa gaattaaaaa ttgttaaaga
    14881 agcgtatgaa aaagaaaaaa atattatatt gaaggatgaa ttaagaagag tattatatga
    14941 aagtaataat ttaaaaaaag aatttgtaaa tattgaaaaa ataaaagtag atgaacatgg
    15001 taaggatatt tatttgactt caatagctgt tgctggttca aggtttagaa atagagaata
    15061 tcttgaaaaa gaattggaga aaagtaagat atattattta actagagaga aagataatct
    15121 atatgatgaa aaagctatta aaatagttgg tgaaactgga tatgtgatag gctatgtccc
    15181 aagaaaagaa aactatattt tgagcaattt attagatgga ggaaaactgc tgtattgtag
    15241 agtaactgaa tataatttat atgaagattg tatctacgca aatgtgtact tgagttataa
    15301 agatgtaatt gaaacagttg aaaactcttt aaagatggtt ttagataaga gtagaataaa
    15361 acttataaac taagacgttt atatattgct tagatggtgt aatcctaact gattggttat
    15421 tttttctgga attatggtgt attttatgag caatgctaat gaatataaaa taaaaaaaca
    15481 attgttttgt atatatgatg tatataaata tatttatata ttcatattta caaaacaatt
    15541 gaaatttatg ttattttata attttaaatt tagatggaat tttaacttct gctttatctt
    15601 ttattttaga agcttgtttt ttaactttat ttaataattc agattcatct acaactaata
    15661 cttttctttc cttcatcaac catttaccat cacaaatagt agagtctaca ttttcactag
    15721 tcatagaata aactatattt ccaattggat tatgcattgg taaacaatga attgtatctg
    15781 gatttagaat aatcatatca gctttttttc ctacttcaag actaccaatt tctttttcta
    15841 gtaaagcaca ttttgctcca tttatagtag ccatttctaa gacttcttca gctggtacga
    15901 cagttgggtt taaagttctt cctttatgta ttagtgatgt aagatacatg tccctcataa
    15961 ggtccattct attatttgaa ggagctccat cagtaccaat tgaaacaggt attccttttt
    16021 ctaacatttc aggaattgat gcaaatccta gaaccacttt catagctgaa cctgggtcat
    16081 gagaaacttt tacatcatgt aatctaaata aatcaatttc cttatgagta agccaaactg
    16141 tatgagcagc taaaagattt ggtcctaata cacctagttt atcaaggtat tcaactgttc
    16201 caactccatt gttttgcttt acaaatgaaa tttcatcagc tatttcagca atatgcatat
    16261 gtatacctgt attgtattta tcagctagtt ctttagttct aacaagtaat tcttcagttg
    16321 tattaaaaat agttcttaat gcaaaccata ttttaattct ctcattagat gtattattaa
    16381 acttatggaa taagtctaat tgagtattga tttcctcatc agtagttttt tgccatgcct
    16441 caggaagacc atctcctgta tccataactg atttagctaa acaggctcta agtccagttt
    16501 tatcaacagc ctcaaccata gcatctacat attgaccacc agattctaaa aatgtagtga
    16561 caccagattt tatcaattca acacaacatg cagtagatga aatcaaggaa tcttcataat
    16621 tgaagctact ttcgtatggc caaattctct ctctaagcca agttaaaagt acaacatcat
    16681 ctgcaagacc acgtcctaat tgttgagata agtgaacatg cgtattgact agccctggca
    16741 ttagtatttt tcctttagca tcatatatct ctgcatcttt atctacaata ttaatatcaa
    16801 acttaccaac ctcttttata atattatttt caataagtat gcttccattt tcatatacct
    16861 ctctatctgc attcatagta attagatatg cattttttat aagtaattgt gacataaaca
    16921 ccactccttt tctatatatt tttatatcta tatattatga taataaattt tctatacaaa
    16981 gttaatagca aaatcaattt acatataaag tgattttgct atttataaat ttaaaataaa
    17041 taagatttta ttctatataa tttaaaatat tgacctagaa ctatttaaaa atattaactt
    17101 agaactaagt ttacaactgc aagtaaacct ataatccaaa gtgtaatact gattttatca
    17161 gtttctttag ctactacatg cattattata tatgaaataa atccaaagca taaaccaata
    17221 cttatactat aagttaaagg catcaataaa actgtaacaa aagcaggtac agctattttg
    17281 ctatcagcaa aatcaatctg ttttatgctt tcaaacatga atacaccaac cattacaagt
    17341 gcaggagctg cagcgtaagt aggaactata ccaagtaatg gtgtaaatat aagtgtagct
    17401 aagaataatg ctgctgttac tattgaagca agtcctgttc ttgcacctgc tgcaatacca
    17461 gaagcagatt ctccaaatgt tgtaacagta ctagttccta agaatgagcc tattaaagta
    17521 gaagaaacat ctgcatataa cattcttcct aaatctttta tttcaccatt ctcatcttca
    17581 agacccattt gtttacaaca agtaataaga aatgctaatg aatcaaacat gtctataaac
    17641 atgaaagaga atataggtcc aattaatgag aatttcaatg cacctacaat atctaattta
    17701 aaagcaactg gtgctatact aggaggtgtt gagattattg ctcttggcaa ctccacaaat
    17761 ccaaatataa cagatattat tgttgcaact actatactta tcaatattcc acctttaact
    17821 tttttatatt ctagtattgc cattaagata agagcaaaaa gggctattaa aactgttggt
    17881 gtaaaatctg caagtgaaac tatagtatct ggattagcta ttataacacc catatttttt
    17941 aaaccaacga aagcaaggaa aagacctata cctgctgaag atgctactgc caattctcta
    18001 ggaattgcgt ctgcaattgt ctttcgaaga cctgttactg cgagtgtaaa gaagacacat
    18061 ccagatagaa aaactacccc aagacctgtt tgccatggga ttcctgcacc tataaccaaa
    18121 gagtatgtga aaaatgcatt taatcccata ccaggagcta gagcaaatgg tgcatttcct
    18181 aaaatcgcaa atataagtgt accaatggct gtagctagta tagtaactgt tataagggcg
    18241 gctttatcca ttccagcttc agataatatt gctggattta cgaaaactat gtatgccatt
    18301 gttaagaatg tagtaactcc acctattact tctgttttaa ggctagagcc acgttcgcta
    18361 aatttaaaca ttttgtctaa aaagcttaat gatgaattat ttccacctgg ttgtacattg
    18421 ttagtgtttg gtaccatttt tacctccaaa aaactttaag atataaacaa aaaaatatat
    18481 attcatatag atagaataga acatatattc agagttgaca ttctattcat agaaaaccca
    18541 tttaaggtga gtttgtagaa actattagac cgtattacta atattatatg aacattttct
    18601 atttgttttt c
//
LOCUS       DAEMHZ010000072        18580 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000072 DAEMHZ010000000
VERSION     DAEMHZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.072"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(614..1165)
                     /locus_tag="KPK72_002313"
     CDS             complement(614..1165)
                     /locus_tag="KPK72_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG2489522.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDIPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(1316..1579)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2015..2413)
                     /locus_tag="KPK72_002314"
     CDS             complement(2015..2413)
                     /locus_tag="KPK72_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489523.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIKSSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     gene            complement(2769..3254)
                     /locus_tag="KPK72_002315"
     CDS             complement(2769..3254)
                     /locus_tag="KPK72_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2489524.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(3337..3430)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3786..4169)
                     /locus_tag="KPK72_002316"
                     /pseudo
     CDS             complement(3786..4169)
                     /locus_tag="KPK72_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      4395..4492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4547..4723
                     /locus_tag="KPK72_002317"
     CDS             4547..4723
                     /locus_tag="KPK72_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489525.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            4949..5149
                     /locus_tag="KPK72_002318"
     CDS             4949..5149
                     /locus_tag="KPK72_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2489526.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   5513..5740
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5579..5607
                     /rpt_unit_seq="ctttacataccacatagttgatataaaac"
     gene            5967..6149
                     /locus_tag="KPK72_002319"
     CDS             5967..6149
                     /locus_tag="KPK72_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG2489527.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            6220..7065
                     /locus_tag="KPK72_002320"
     CDS             6220..7065
                     /locus_tag="KPK72_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2489528.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(7289..7453)
                     /locus_tag="KPK72_002321"
     CDS             complement(7289..7453)
                     /locus_tag="KPK72_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489529.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(7994..8326)
                     /locus_tag="KPK72_002322"
     CDS             complement(7994..8326)
                     /locus_tag="KPK72_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG2489530.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(8570..9211)
                     /locus_tag="KPK72_002323"
     CDS             complement(8570..9211)
                     /locus_tag="KPK72_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG2489531.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(9192..9485)
                     /locus_tag="KPK72_002324"
     CDS             complement(9192..9485)
                     /locus_tag="KPK72_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2489532.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            9864..9923
                     /locus_tag="KPK72_002325"
     CDS             9864..9923
                     /locus_tag="KPK72_002325"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG2489533.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(10439..11191)
                     /locus_tag="KPK72_002326"
     CDS             complement(10439..11191)
                     /locus_tag="KPK72_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2489534.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(11250..12905)
                     /locus_tag="KPK72_002327"
     CDS             complement(11250..12905)
                     /locus_tag="KPK72_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489535.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSDIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKSYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(14178..15257)
                     /locus_tag="KPK72_002328"
     CDS             complement(14178..15257)
                     /locus_tag="KPK72_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2489536.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(15341..15997)
                     /locus_tag="KPK72_002329"
     CDS             complement(15341..15997)
                     /locus_tag="KPK72_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489537.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            16319..16525
                     /locus_tag="KPK72_002330"
     CDS             16319..16525
                     /locus_tag="KPK72_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489538.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(16678..17106)
                     /locus_tag="KPK72_002331"
     CDS             complement(16678..17106)
                     /locus_tag="KPK72_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2489539.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(17582..18520)
                     /locus_tag="KPK72_002332"
     CDS             complement(17582..18520)
                     /locus_tag="KPK72_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2489540.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKKLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         6415 a         2936 c         1924 g         7305 t
ORIGIN      
        1 taatattttt tttattatgt tattttaata gatttatttt tcttttttaa gcatagagat
       61 gtatttttcc atatattcta tttctattat agtttcgctt atttaattca cccaaaattt
      121 cacttaatat tagaatttta aatcttttac taaaacagac ttttatagtt ctattaaaat
      181 tttttactaa actttatttt atactatgaa ttatctattt tttttggcta aatttaagca
      241 agtttttcac aaaaaatata tacattacta tatttttaaa actttataaa tatataattg
      301 atttaattgg aaattttagt gtattcaaaa aatgagaaat cagaggagat taatataaaa
      361 atttatcttg aaaagtcaat taaatatatc ttatatttaa taactacaaa tgagatacat
      421 ataagaatat tatataggta tgataattta tccgttaaat taagtgtaat tggtactata
      481 taaatatttt attaaaaata aatatataat accaaatctt taaataacat aagaataaaa
      541 tagttataat ctactttatt cttatgttat ttagttatta taatgcttta taaagtaaaa
      601 tattatttcg tgtttaagct tgataactaa ctcttcttat tgatagacta acaatcttga
      661 ataaaaatat tcctgcaact agatttatta aaacagctgg taatactact gctaagaata
      721 aagctgtaaa tgaagcccct ggaagaccaa ctatagcttg agctgctaat aaaaagatag
      781 ttccacttac aagagttcct attggcatta ttattgcaac tactacagta tcttgtgttt
      841 tcttagctaa tttttttata aaaggcatat tataactaat attcattaat aaaaatacta
      901 tatttgctgt aagagtttta tctattacat taggaatttg acctcccgga aaacttgatg
      961 taagtgctgt aaatattcct gttactatac ccgctatcaa acaagtttta taatcatctc
     1021 tatttaaaac caatattaca aataacattg ctagtggtat atctggcttc attggtaatc
     1081 ctatagctgg tgtgatttga tgtattaata atcccatcgc taaaagtatt gcatttaaag
     1141 ttaatttttt tgtatttgtt ttcatttttt gtacttcctc ctattttttt aattttcaat
     1201 ttattttttt gccaacctac tatatcatca tctttagctt ttgaaattca taacatcttt
     1261 ctcaccacct tctatctttt aaattttcag taaaataaaa aactccgtcc taaactaata
     1321 ggacggagat tatccgcgtt gccacctaaa ttatataaaa attaatataa aattcaattt
     1381 tcatacatct cattatcagg tacttacata ccttattcgc tataacgtgc gactgacgat
     1441 aaagtctact ctcaaaacta tgatttcaat ttattcctct aagggccatt catcaaattt
     1501 tttcttgcta agattccacc atcctcagct ctctatactg aacctaaatt taactactct
     1561 tccttgtcat aggatttaat ttattgtatt gaattttgtt gttaaaataa tgatattctt
     1621 ttttctaaaa aatgtcaata cctttttata aaaaatatat tttgtaatct attttttata
     1681 aaaaaagtaa attatactat acatttcttt tcatatacaa aattctatga ataattactt
     1741 tcatggtcta atagtctgct ctttgactct aattcttctt atattaattt tgtgaataac
     1801 tgttgatgta attttatatt ttgattcatg aggaattaaa ttcttcaaag ttaattaagt
     1861 acatttatca ataaatttac ttaattaacc atcatttaat actcattcct gtatttatat
     1921 gcctcctttg aaacagaata ttactccatt ttaattacat catattatta aatagatacc
     1981 aatgattaaa aaaatgatat tcttttaatt atttttagta aaattttttc ttctttcttt
     2041 taataaatat tagcactact aaaactataa ccaaaaatat gactattatg gacaatatta
     2101 attttgttag gatattatgt cttcttgcgt tttgtctttt atatttattt atagtttgat
     2161 atccatactt tttagttctt atacttcgtt ttttcaactt tactttactt ttattatatt
     2221 tcctactttt atgtctactt ttagatttaa tagttttaaa tttagtatta tcatatgtat
     2281 aattctcttt tattgaagaa atgtatacac tagacttaat aatagcttct gcttctactt
     2341 gtaatggatt tataatgctg attataagta gaaaagatat agctatccca atcaattttt
     2401 tcttcttatt catattttcc ccctcataaa aatttttaac tagttcatta taatatatat
     2461 ataactaaaa attacaaatt ttttcaagtt tcttatatat cttattatct ttaatattat
     2521 ttttattatc ataactttta ctttttttaa gatttttcaa agtagtatta atttatgata
     2581 cttggtgtat aaagtataag ttaaatctac tttcaattaa atttaccatt tataattact
     2641 ttataataaa aataaattag ttaatttatt ctctttagtt ctgttataat gtttctacaa
     2701 aaaagaatcc cattaatggg attctttttt gcgatttatt aatatattta aagcttgcta
     2761 gtatcgtatt ataaacttaa atgctcttct atgtattgat tatatacata tccagaatat
     2821 cgtttttcat ttttattgat attaactctt gcccattttc caacgctata acatacttca
     2881 actttaaaac catcagggat tgtagttata actttagatt ctaatgttct atcatttcta
     2941 agttctaaac ttaatccatt tgttcttata tttcctatgt aattcttttt tctatttttt
     3001 attgtatcat tactattgtt tttagcatta cttaatcttt ctaaatattt ataaagtaac
     3061 caacctttgt cataattact ctcaacttct gcccatattc catctacaca ctttaactta
     3121 acaattttac cttctggtat actttttaat ttcaaactat ttatgtctgg tttttctctt
     3181 aaattcaatt taattttagt cttaaaattg atgctagatt tgcgaaaatt ttttaacatt
     3241 aattcagata tcatcttaag ccctcctctt gccaaatatt aagttattaa aatatataac
     3301 tctaaaaata cttttaacaa aaagaattat tatctttttg gcaactggct gacatgacct
     3361 ttattacata aagattttag tccctatagt tttgcgtcat tagatttatc taatattgcc
     3421 tatatctgaa taataacata atttgaacaa ttaaaaaata atattacctc aaattttata
     3481 ttatttaaat ataaattaaa tttttaatta ataataatca aaaaatatct ttatatttat
     3541 tcaattactg ttctttatag tcaatataaa agacagtaat ttatctatag tgttacttat
     3601 attttatata tatggctatt tttcaacatt ttacaatata tataacattt atttttttaa
     3661 cttttgtcag taaaatgtca gcaaaacact ttatccacaa tttttatata agttttccac
     3721 agcttatccc cagtattatc cacataaaaa aggtagctct tacattatta gctacctttt
     3781 atttactaat cttcatattt ccagcttttt atccaacttc ctatttctat tattttttct
     3841 tcagttattt cttctccatc atgtaatttt gaaattaatt ctgataaagg attgttatgt
     3901 aaacctctaa aaactctttt tccctctatt ttgagatatt ctctctcttt tattaatact
     3961 gtatagtgta catgcctccc aattctctat gatattaaaa aacctttttt ggttaatcta
     4021 gctaaaagta taattgtagt tgtatgcttc caaccatatg ttttttctat ttcatctgca
     4081 acatcttttg atattacagt tttataacca gtattccata tgcatttcat aatttttaat
     4141 tctgtatctg gtaatttttt atttagcata atatattcct tctttcaatt tatgtcactt
     4201 cttcaaattt atctagctta aattataata ggagcttttt agaacaaaaa ctatatttaa
     4261 tttttaccag gtactctacg tgtttagtct gtttttaagt gtaaaataat gttcatatct
     4321 ataaatttat ttaactttca aacaatcctg ttgaataata actaattcta ttgtattatt
     4381 ttagttgtag aataaaacta aatcggcaaa actagagaaa tttagtgacg caaagctata
     4441 gggactaaga cttatataaa tatcttatga gttatgtcag ccagttgcca aagagatagt
     4501 tttctttttg tttttatgaa agttttttag ggggataata atttatatgt ttggaaataa
     4561 aatggataaa tgtacacatg tattgactgc ttatattagc agttcgtatg attattgtaa
     4621 ttttttagat acacagttag atgattttat attagagtac ggagaaaatg tagtagagtc
     4681 ttgtttacac caagtaatgg tattggtaag taagtataat tagatagaca ttgatttcta
     4741 gaaaaccctt tttagtttta tctacattta tattaatata tagcaatgta aaacgcttaa
     4801 atatattttt aatattaaga ataattaaaa tgttaactaa aaaatataat taagtagaca
     4861 tttctattaa tctatgatac aatagtattt gtggtaatac acaattaaat caagtatatc
     4921 ttgatatagg ttaataggag tatttttaat gaataatgga atagtgaaat ggtttaataa
     4981 tgaaaaagga ttcggtttta tatcaatgga aggtagagat gatgtgtttg ctcatttctc
     5041 agctatacaa acttcaggat ttaaatcatt agaagaaggt caacaagtaa gctttgatat
     5101 agttaaaggt gctagaggtc ctcaagcaga aaatataact atattataat atatagattt
     5161 ttcgggaaag actcttttta gagtcttttt ttattgttaa tatatgtaag ataatcattg
     5221 gtgaagtaaa taaggtatat tgagtaatgt tcttgatagt ttatatgcta atgaatttca
     5281 aaaaaataag cactcctata aaaagagtgc tcttaatgta aatataaacc ttcattggtc
     5341 cagcctaata ccccgtattc tccaagttct aactcttttt tatatacatt atcactttca
     5401 gccatatcta acacaatcat ttcttcttta gaatcaattt gtaaaatatg aggactgttt
     5461 gttgtgacaa ttatttgtgt ttttgggaga attgctttta atgtaataaa aattttacat
     5521 accacatagt tgatataaaa cttaagacta gcagactcat aagcaattag ttgcctctct
     5581 ttacatacca catagttgat ataaaacaaa gaatttggat tatcatatga atacattgtt
     5641 gctactttac ataccacata gttgatataa aacttagtta gtaaacatta taatgatgag
     5701 ttgcttagtt gctttacata ccacatagtt gatataaaac cccaaaataa acttagcatc
     5761 tccaatgttt acacatatac aatgctctta aaattgcagt gaacgcgcaa tagtgcaatt
     5821 gataatactt attatatatt ctcaatgcct tatatttcaa ctgctaaacc tcaatttgtc
     5881 acaaatatcg ctcactgcaa aatttctata tttttattat atcataacat ataattaata
     5941 ttttcaatta atttttacat ttaattatgt ttaaattcta taataatgca actttaatgt
     6001 ttctttctgg aactaatata aaaactattt cagaaagatt aggtcatgca gatataaaaa
     6061 taactatgaa tagctactct cagtttttag aagagatgga cagagaagca tttgaaaatt
     6121 tgagcaaaat actatttatt atattttaaa gaagatgact tattcaatat ttgatgctag
     6181 aatataatta ataaaaattt aattataggt gattgtatta tgaatattat taaaagcata
     6241 actttactct taacattatt aattgtgata ttaatgacag gctgttcccc aagagaaagt
     6301 gctagtgaag ttgcaaaaaa atatattgat aagatgaact ctggtgacta tgaaacagca
     6361 tatagtttat taagtaaaga tagtaaaaaa aaaattagta tagaaaatct tgaagaattt
     6421 caaaatattt tagttagtac aaaaagaata attagttata aaataggtaa agaaaaaatc
     6481 ataaaaaaat ataaacatga tggaaaagaa tataaaaatg ttgttaaatt agaagaaact
     6541 tttaatataa aaaaccttct ttataaaaaa gatgattctc taaaagtaaa tagatatttt
     6601 gtagttgaaa ataacaaata caagctatta ttgtatgaaa gtttcaaaaa agaatttggt
     6661 attaatagta tggaccttgc tcaacttaag cttaatgatt ttggactaaa atactttaaa
     6721 gaagttgacc ttatactaaa aaaagctatt gctattaatc ctactgattc aaagctttat
     6781 tatgcccaag catgtaatta tatgaatttt gatggatttc ctggttattc agatacaaca
     6841 tataatgctt tagattcaat taataaatct ttaaagtatt tagataaaaa tgattctgac
     6901 tacaaaaaaa atgcaagtaa tatatataac ttaaaaggag ttattcttat gtttcacaaa
     6961 agatttgatg aagcacaaca atatttaaat aaagccttat actttaacaa agataatgaa
     7021 gacccaaaat ataatctaaa tacaataaaa aaacatctaa actagatatt taatttttga
     7081 atttataaaa tattttttta tgtataattc agcactgaat cataatttct actaaactaa
     7141 ttacaagttt aaattataaa gacttatttc tatataaata ttactctata acaataagtt
     7201 tcttatttaa attttaaaat attatctaaa aatcataaaa aagaaggtat aaagagctac
     7261 cctcaagctc aatatacctt ataaatatct aaacttcatt ttttactaat ttttctatat
     7321 ctttgataat atgttcttta gatttttttc cagctatggc atcaaaaatt gcaacagcct
     7381 ttttctctcc aaacttttct gctacatcat taaagtctaa aaatctaaca ctttcttttg
     7441 gtttaccaaa catataacac ctctcataca tttttatata tttattataa cttctataga
     7501 cttatttttc accaataaac gttagacaaa ttagtccctt tattttttta aactagcttt
     7561 accttcaaaa ttcttaacaa aaacagttta ttctactcta taaataaaaa tagacaattt
     7621 aatttgtagg aaactgataa tagaatacag taaataaaac tttgtttaat ctatctgaaa
     7681 aagttatcta ttaaaactgc tatgaattta taaagaatat tctagttcat aaaaatacaa
     7741 atatgtaaat ataatcacct taatattaat ttatttaaaa taaaaaatgt ataaaagatt
     7801 taataatgac aatatactat tctcaataca atctatgatg taaataattt aaaactacta
     7861 tattatttaa aaatttatat ttatatatgc aattctaaaa acttaataag aagtgaactt
     7921 agaaaataaa gcttcatttc aaagacttaa atagactatt attattagtc taaataaact
     7981 acgtctatca aaattatata ttaaattctt ctaaaatttt tattgcaaat cttgcgtttg
     8041 gatatgtaat agacccgcca ccaattacac ctatttttat agcttctata atttcatctt
     8101 tacttgctcc agaattaata caaccctcaa tatgataaca aatacactca tcacatcggc
     8161 ttaaaattga aatagctagt cccattaatt ccttattttt tttgcttata gccccttctg
     8221 aataagtctt atcatcaaga cttccaaata ctctaaaaaa ctcatctgtt tcgcttaatt
     8281 ttttattgta aaatccccta tattctttca tttctctagt attcatcttc tacctcctat
     8341 gtttttctta taagaatatc acaccgtact tatattttca atattaaata caaattttca
     8401 ttttatgttc attagcctaa tttatattat tttttgaaat ctcaataagt tatcatataa
     8461 gtaagatttt ttcagaacta aaatagaaat cttagtaatt tctttattat ctatctcagt
     8521 tctgaaatat attattctta tccaaacatt attatagtga ttaattatat tagttatttt
     8581 tacctgaaat tttatacatc aaataagaat atataaatgg tagtattaca attacaaaga
     8641 ttacaaagat attataaaaa ggaatattaa taaatgcatt aataattaat aatattccac
     8701 caataagcca aatccatcca gctaaatggt gtgtcttatt ccaattatct tcatcattaa
     8761 gagtccatgg taaaattatt cctactgagt aatttcgttt acatttagga agatagtttc
     8821 caaataaaat aactattatt cctaaaatta atcctgtata aaaattagac tccaaactaa
     8881 ctcctgttga atataaaacc atcattattt gtataattac tgaaagtata gcaactcccc
     8941 attttgataa attttgtata acatttgaaa aattagcttt cttaggctca ttctcaagtc
     9001 taaaccatgt atacaaatgg atacatagca ttaacaatgg tacaccaaaa gctgctataa
     9061 cctttggagc ataattatct ggatttccat aattatcaaa atgaattgca acttcatttg
     9121 gtaatctatt ataaaaatat attgatacaa taaatggtat aaaacatatt aaagtagata
     9181 aaataaattg tttatttatc gcttttttca ttatcttttt ctcccttctc cataaattgt
     9241 gaaaaccaaa gcataacctc ttcgaaaact gaaatattta tttcgtagaa aataaagttc
     9301 ttatactttg tttcaaatat gagtcctgcc ttttttagtt gagatagatg ataagaaatt
     9361 gtagcattcg tcatattaaa tttttcacta atttctcctg ctgacatttt attctccctc
     9421 aacataacca atatttctct tcttataggg tctgaaagag ctttgaatgt ttctggaaac
     9481 cccaacacca tcacctcgct atttagaatt tcatctaaat agaatataac atagcatcca
     9541 ttttatttca atagctattt agaaattttt ctaaatagtt attgaaaatt ttcatattaa
     9601 gattctcaat ttacttaagt cgcttttatt taatcttact ttatctaatt tttttaactt
     9661 aacaaaagtg tattgcacca taattgttaa agcaaaaacc taatgattga ggtacttata
     9721 tatataacaa tacttttaat tttaaattaa aagtagctaa acccaattta aaaagtagtt
     9781 aagtcctatt taaatagaaa agaatgaaac gagaaatata ctatataaaa gtacataaaa
     9841 acatctacta attagataca actataacag aatatattta ttattataat aacaaaagaa
     9901 taaaaaaaat taatttagca taaccttgtt aaatatagat ttgaatatga atttaaagtt
     9961 atgtaaaatt aaaaatacag gaatgaaaat tccattcctg tataaaatct aacttttgag
    10021 tctcagtaca atcttatttt gagacaatct tttttgtttt atattttctt atatttaaaa
    10081 tataacgcat atcttatgca acatattcat atttttcttt atattctttc atcgcatcat
    10141 tccaagcgtc ttgtactgtt acacctcttt tattagcaat tttttgtgcc agttcaatta
    10201 tcttcatagc tcttcttgat atgttcataa acattcctcc tacaaattac catatatttc
    10261 atatatataa atcaaggtta tatttttatg atatataatc cttctattgt aagattacag
    10321 tttatatgtg tattatatat gaactatatt agaagataat tagaattttt ataaagctat
    10381 atgattagat taaatataaa agctttacaa ttacttataa atttaaaaag tctataaact
    10441 aaactaatgt ttgatatacc aattttacga ccataaataa tgccattact aaaaatattg
    10501 gttttatcat ctttgaacct ttctttatag caactttagc acctacataa gctcccatta
    10561 tcatcactaa tgcaaacact atagcaattt tataatctac ttgaccattt atcatgaata
    10621 ataacaatga agttatatta cttgttaagt ttaatatttt tgtatttgca gaagcatgta
    10681 aaaaatcata tccatatatt tttataaatc caaaggtcaa aaaagttcct gtacctggac
    10741 caaaaaaacc atcataaaaa cccattataa gagccataag catccctagt cttaaatttt
    10801 tcttattaac agcttcaaaa ttatcttctt gacctaaatt tttagaaata aaggtgtaaa
    10861 tagccactat taaaatcata accacaacca aaacatttaa tatctcttca cttattgata
    10921 atacacatct cacacctaga acactaccaa taatagtaaa tggtactaat ttttttaaaa
    10981 ggttgttatt aatttttcca gactttgcat atctataggc actacttata catcctgctg
    11041 atgctgcaaa tttatttgta ccaatggcaa gatgtactgg cactccaatt gccataagaa
    11101 caggcatgct tataagtcct cctcctcctg ctatagaatc tacaaaagca gctaaaaacc
    11161 cccctataca taaaaaaata actgaaaaca taaaattcct ccatcattct ctttcaattt
    11221 ttcttacaat atatattata taatcttatt taatacttat tatccagcat attctttcta
    11281 tattcatttg gagttatact attaaacttc ttaaatattt gtgaaaaata actactgtta
    11341 ttaaaaccta ctttcttaca aatatcacta atactcatgc ttctatctaa cagatatttt
    11401 ttagattctt ctatcctaag aagatttata tactcagtta ttgaaacttt atttctttca
    11461 ttaaaaatag aactcaaata atttggactt ataaaaaaaa catctgatat actattcaaa
    11521 tttatatttt ctttataaga tttttttata tattcgttta tattttcaat tagatttcct
    11581 ttatcttttc tccaaaatgc tctattctta tcaatacact ctttggaaaa ttcaaataat
    11641 acattccata aatcttcatc actgtttaca agtgatattt ttttattata tttatttctt
    11701 aaataagata taagttttaa atcctctaaa tctctaagca ctgttctaga caatacaaat
    11761 atcatttcct gaatagtatc tctaacatca ttatatctta atttgtttgt atctattaga
    11821 ttataaacac cttctaaatt catattttta atttcttttt caatataaag ttctaattca
    11881 aagggatatt cttttgaaga attattgaac atatctatcg gattaataat atactcttct
    11941 tctaataatc tactatttgc ttttttaagt gcctcttcaa gtcttatatt ttcttttcta
    12001 ctcttcaata tctcctcatc ttttatacta agttgatttt caaaaaaatt cctcttagct
    12061 atttcctgaa taaattcaga catcattaat aaattttctt ttaatacata taatctactt
    12121 ttaggattta atggaaattc attgtaaatt ttgtttatgt attttatatc aatatcagaa
    12181 ctacttgata attcagataa atttttatct atatcaatag acttatatac tataaaatta
    12241 ccacattcta tatatccaac tatattttct tctaatacta taggtatctc taaactggaa
    12301 acaccatact cacaatggta cactttttca aagttatcat tatttatgct tttaacaagt
    12361 tttttagtac actgttttat atttttacaa atatcgcaaa atttattttc tttcttagtc
    12421 tcaaatattt ctttatactt taaatcatat acagatattc taagcttcaa tatattagat
    12481 atttgatttg caagattttc aattatgttt acattaacta actcctgtat attgttttca
    12541 gaaaatacta taggtatttg tctcaaactc gaaaatttat gaggaacata agtaattaat
    12601 attactaaat caattttacc agtgtttata atttcatgtt ctgaatatgg tgggcaataa
    12661 actatagatg tttcctttat attgaccttt ttaccatcaa ttatttgtat tccttctccc
    12721 tgaatcacat ataaaagctg ttcttcactt aaatgaaaat gcttttcttg gcagtttccc
    12781 ggaaaaaatc ttacaattcc agcactaagt ctattgaatt gtgtttttga tggttcgtgt
    12841 atccacataa cctctcccca atcaaatttt tgtacttcat tatttgactc actaaaatac
    12901 tccataagct cacctcttag tttttgtcta aatatttttt cttcccttct tatattaaaa
    12961 taatatcatc atcattaact ttcattacta taaaatcatc atactctttt aatactcctg
    13021 taattttagt tgcacttagt ttactacaat tcagtttttt aatatattga taaattctat
    13081 aattactttt ttattttgta aaatcagtgt tttcccaaaa taattatatc tataaatgaa
    13141 agtattttta ctgcttacat caatatatgt tatcataatt ttccttttta ttgcatatat
    13201 aaataactgt atataacatt gtccaatact atatagaaat acatctaaga aagaatatta
    13261 ttaaattgtt ccaattagta ttatttttct tatagaaata aaaaaactgt tggattcttt
    13321 tagaaaaatt attttttaca tacctttttt gcttagatag tctatcttct cagctatttt
    13381 gtatatacac tcttctagtt catgatttta ttttaatatc ttgcttatat tgctgaaata
    13441 attgaattct tttatctatc atattgtctc ttatatcata aacatattgt ttcttatatc
    13501 acaaaaatga aagcattctt atatcttcaa tatataaacc tgcttttttt aatcttgtaa
    13561 taaatatagg atactctata ttctcttcct agaaataact aataccactt atttttctat
    13621 tatatagaaa gtaataaatc tttttcttaa taaaattcaa tagtaagtgg aataaaattt
    13681 tatatgaata agaacattat aaaaatactt tatacaatag tcaataaaag atattagctt
    13741 caaattatgt taaagttgta ttatttaaag tgaaaaaaca aataaaaaag aaataatagt
    13801 ataagtagaa aaatatactt aaaacaaggt gaaacctctc ttgtatctaa atcaagtaga
    13861 gaattaaatc taaaaaagaa aataaatata aaagatgact cctgtacatt acaagaatca
    13921 tcttatggtt tcataatcac tccatggttt ttaactgtct gatataaagg tctcagttca
    13981 ctcatagcat gaccaccttt tccgattgct gaccccgtca cttatggaca aaatgtccat
    14041 aagtgatagt cctctcttaa aaccctctaa atagatatat ctcaagcacc gcattgaaac
    14101 cagttgctgg aaacaaactg aagggttctg gaatagtaaa agaaattgcc accatatcca
    14161 cgttactgtc ctagttgtta ctttgctaac ttcggagcga tttcctctaa caatacgcag
    14221 tcaaattctg cctttttgct aagaaatggc agatggtctg catctggtat aaaaaaaatc
    14281 tgtttgtctg gcgccactat ttcatcaaaa aatttcttcg ctggaagata ggggctatta
    14341 tagtcatttt ctcccatgat gtagtataca gggacctgat agtcaactcc ataatttctt
    14401 atgtcagctt cttccactaa aaaccgcaat acatcacctt gattgtcaat cgcatttgga
    14461 ttgacaatgg ccgtattttt taactctctc aatgaataat acggggttgc cagtatactg
    14521 attatattgc ccaaatccat atttatagaa agcccatact tgctttggta tgttcttact
    14581 gtcataagag ctgccaaata gcttggacca tattttgctg gcggatatgg tgcaatagct
    14641 tccagcgcct tgatatcttt ttgcttccct gcagctttca ccatttcaag tgtcttttca
    14701 tagcccacgc gctcattgtc caccatatta acggtctgcg caatcccaat gtacgcgctg
    14761 taatcctccg gataggtttg cacagccata gttcccagca ctgttcccca gctatgacct
    14821 ataaggataa ttttctcctt gttgaactct tgcttcaaat atgctgtcac ctcatggacg
    14881 tcttgcagaa cacgctccgc gctcatggtc tgcaaaactg ctgccggatc gttcgcaaag
    14941 tatgttttgc ctacgtttcg ctgatcccag ttcaccacag taaaatcatt ttcccactca
    15001 tactggtaca gatgtaaaaa tggcatcatt gaattaccag gacccccatg aacccaaagg
    15061 atgatggggt tgtctgcgtt ttgtccccgg atatataacg cctgctgtat gccgccgatc
    15121 tccaccattt ccatgcggtc gattccagga gcttctattt tatggcttgt acgtataatc
    15181 gtgggggcta tcagacgcaa accggcaaac gccagaacaa aaacagcaac tagtatgcaa
    15241 agtgtgatta atatcatttt aagtaccttc tttcatgaat ttttttagcc atagatatct
    15301 cctctaacat taaaaaaaat atgttgctat tttagttttg ttactctacc tgtcccattg
    15361 gtttcctttc ataaaaaata ttaattgatt ctataatctc atcttccagg atatcaactc
    15421 ctatttgtct cataagaaac tgtaagaatt gaaaccgttt acgaagtgta ggaaagtccc
    15481 cctgaaatac atctgtatca caccaaaagt taaagagcag caaaattacc tctgcgcatt
    15541 catctgggaa ctctgtgatg atgcttctat cctctatgcc ttctcgtaaa acatttgcta
    15601 atattggtgc tagttttttc aaattatcct gtgtaaatgc cagtatgata tgcggacttt
    15661 ctatcgcgct tgtaatcatg ttactagatt cttttatcac ctcatccgtt aaatgccgcc
    15721 taactaaact tctaagcttc tcttttgctg taagtccatg catttcaccg agccattgat
    15781 ttactgtctc tattatctgt tcaaagcgcc tttccatgac cacattaaaa atatcttctt
    15841 ttgaactgaa gtgatgaaaa attccgccct ttgacattcc cgaagcatca acaatatttt
    15901 gcatacttgt tttgtcatat cctttttcag caaacaattt tgcagaaatg gtaatgatat
    15961 tttcaatagc ttgctttgga ttgttctttc tagtcattat ataactcctt cttattaaaa
    16021 accgactgtt ggtttttatt atataagcta ttcctacttc tgtcaagcaa cattatgttt
    16081 atggcaaaac aataaggttt ggtggttaga caggtattaa ttagactaat gcgactttcc
    16141 cctagatgga acccttggta aatttgcaat ctttaccggc tttgattata tctgtaagca
    16201 aggcaggtat ggccacacct gccctgtttg accctcggat attcattcct atccctcctc
    16261 tccttagcca ttacaccaaa acgccgctca ttgtctccaa tggcggcgtt ttaaagatat
    16321 gatttctcat gctgctgaaa tatgcaaaaa atataagatg ccctttatta tttttcctac
    16381 aatagaaaag aaaagtattg aagaatgggt taatattgga gcaaatatgc tttgttttgc
    16441 acaagatact acctttatca cttatgttat caatcaatta ttatcttttt ataatgagat
    16501 tgtaccaatt caaaaacaac attaaattat aaatctaatt ttatattaaa agagactaat
    16561 attttatcta tctctattaa ggatattttc ttatgtatca taatattagt ctctcttatt
    16621 attatatact gctatcatca ttagttgcta tttatactat ttttataata tacaaattta
    16681 ttcagctaac gttttgccta attctataca ctcatctttt gcttcttcat ttgggtcttc
    16741 cattgctgta attccttctc caccaataat tgtagcacca gcattttgca tacgttcttc
    16801 ccagtctctc atccattcac aatttcccca tccatatgaa ccaaataatc caatctgttt
    16861 acctgatacc atagattcta attctgaaac aaatggctcc atatacccct cttctagttg
    16921 ctctgctccc atagatgggc aacctaatgc aaatttatct tcttctttta aatcacttgg
    16981 tttcagtaag ctcacatcta aaacttctgg tgtcttacct tttaacttca caccttcagc
    17041 cacaaaattt gccatttttt cagtatttcc tgttccactc caataaacaa tatatatttt
    17101 actcattttt acctcctgta gtatttttta attaatcaat agttagttat agctaactaa
    17161 catctaaaaa acaaaagatt tctttatctg aaatctcttt atttttctaa atatagaata
    17221 tcacaattga ttttgaaatt caatacttct tgataatatt tatcatttat attttatatt
    17281 cattttataa tatcaaaaca ctgtatcgta tttctaatct ttcatagaaa aatttctaac
    17341 ttatagtact tttgcatctc ttataaatct tttatatgta taagtacata gaaaaacaca
    17401 ccttttccag atgtgctatc tataatatcg tatatttatt tttcaattct tactagctaa
    17461 tatgtttata aattttatca cttaatacct ttatacattg atgtaaaatc ttacacctaa
    17521 gtaattttta ttatttgtct cgactctata taatatagct attttatcat caaatcaaaa
    17581 attatctttt gctattattc ttgtatttat aaagatttaa atataagtat ccatttcctt
    17641 gttccgttct aggtattcca agcgctctcg tacattttat taattgtgca tacagttcag
    17701 cttcatctaa atctctttca aactcctctc ttgcccattc tttaattagt gctaatgagc
    17761 ctgatacgta tggtgcactc atactagtac cactcataat agcaagttta ttatccatat
    17821 aagtcgatat aatatttctt cctggagcca ccaaatctat tgtagtattt gaattactaa
    17881 acttttcaac taaatagttt tcatttattg cacctacttc tattacctca gcataactgg
    17941 ctggataact atactcactt gtactagaat caccatctcc attattacct gctgcacaca
    18001 ctacagaaat attatttttt actgcttgca tgacagcatt ttttaacttc ttatcatctt
    18061 tgtttccccc aagagacata gatataatat caaccttgtt atttacagca aagttaatag
    18121 cattaattat actttgatat gttccagtac catctttatt taatgctttt gctattaata
    18181 atttacaatc tggagctact cccataactt cattattata gttagatgca gctataatac
    18241 ccgccacatg agttccatga ccattaaaat cctcatatat atttttattt ccattactgt
    18301 catcactaaa atttgcacca ccaattattt ttccttttaa aagaggatga gatatatcac
    18361 aacctgtatc tattatacca actacaatat ttttaccagt ataaccttca tcccacattc
    18421 ccctagcatt catttgtttt attccatatg ggaatttatt ttttgcacct cttagatttt
    18481 catttatttc atacggaatt agtttcattc ttttattcat catatatcct cctaaaaatt
    18541 ttattgctta taaaaatcat gagttttatt ttataatagg
//
LOCUS       DAEMHZ010000073        18553 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000073 DAEMHZ010000000
VERSION     DAEMHZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.073"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            524..1567
                     /locus_tag="KPK72_002333"
     CDS             524..1567
                     /locus_tag="KPK72_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489541.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1678..2820
                     /locus_tag="KPK72_002334"
     CDS             1678..2820
                     /locus_tag="KPK72_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2489542.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            3274..4665
                     /locus_tag="KPK72_002335"
     CDS             3274..4665
                     /locus_tag="KPK72_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2489543.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            4918..6711
                     /locus_tag="KPK72_002336"
     CDS             4918..6711
                     /locus_tag="KPK72_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2489544.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(6792..8174)
                     /locus_tag="KPK72_002337"
     CDS             complement(6792..8174)
                     /locus_tag="KPK72_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2489545.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            8432..8779
                     /locus_tag="KPK72_002338"
     CDS             8432..8779
                     /locus_tag="KPK72_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2489546.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            8797..9831
                     /locus_tag="KPK72_002339"
     CDS             8797..9831
                     /locus_tag="KPK72_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489547.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(9880..10929)
                     /locus_tag="KPK72_002340"
     CDS             complement(9880..10929)
                     /locus_tag="KPK72_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2489548.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLNV"
     gene            11204..12739
                     /locus_tag="KPK72_002341"
     CDS             11204..12739
                     /locus_tag="KPK72_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489549.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(12925..13611)
                     /locus_tag="KPK72_002342"
     CDS             complement(12925..13611)
                     /locus_tag="KPK72_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG2489550.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESVHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALNSI"
     gene            13736..15118
                     /locus_tag="KPK72_002343"
     CDS             13736..15118
                     /locus_tag="KPK72_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2489551.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLRYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGVYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTSEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            15520..16788
                     /gene="eam"
                     /locus_tag="KPK72_002344"
     CDS             15520..16788
                     /gene="eam"
                     /locus_tag="KPK72_002344"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG2489552.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(16881..17237)
                     /locus_tag="KPK72_002345"
     CDS             complement(16881..17237)
                     /locus_tag="KPK72_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489553.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(17388..18482)
                     /locus_tag="KPK72_002346"
     CDS             complement(17388..18482)
                     /locus_tag="KPK72_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2489554.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         7377 a         2161 c         3011 g         6004 t
ORIGIN      
        1 agttttaaat tttttatatt aattataatg gatttaatta atcatgttga gtttcaaggt
       61 ctatatgatt aattaaatcc atttttgtta tttataatta ttattgaata tatttttaca
      121 tataagatat aaggtgatag atagaaattg ttggattatg taaataatta atatataata
      181 ccattatggg caataaatac aaatcaaatg ataatatagt ctatttatgt aaatgttata
      241 ttgtatccta agtataatag taagtttttt gatagtggtg tagaaagtaa aaatctacta
      301 gcctaaattg atgtatggat tagtttagga acagtaattg catatcttaa gtttgatgtc
      361 atgttgggct actagaaata ggtcatcttt ttcacttgaa tatcttttct ttaaacatgg
      421 ggagtatcat atcaaaaaca tatgaaaatt aagtgattta tgagaacagt aatattttat
      481 atgatagtat agaatagtaa taaagtatta ggaggaacat tgagtgttaa aaaagtggtt
      541 tggtattgtg aaaaaaagac aaaaaagtga gtcagtaaaa gaagaaggtg aagtaatatt
      601 aaaaaacgaa aaaatattat ctgaagaaaa gttgatagat gaagaaggag ttgtagttaa
      661 tattgataat gaaatattaa ctaaagtaga agtagtaaat gatgacaatg aaatagaaga
      721 aaaaataata gaagaagatt ggttaattag tgaaaatacc ataaaattag atgataaaga
      781 agcaattatt aatgataaaa acatagaatt atgtaaaaaa gaagttcaag ttgaaagtga
      841 aaaaataggt ttaaataagt ttgaaggact ggaccaaaat gaaaattata atctagaaaa
      901 aaatgttatt gaagaaaaaa aagtaagtga atgtttgaca gaagaagatt tagagtacat
      961 aaaagaaatt aaaataaaaa gaggaaaaag tataaaagct ataaacttgt atactaaaga
     1021 agagtgggtt tttgacactc atatacagtg tagtaaaaaa ctcaaagttc cattagggta
     1081 cataagagaa aatttaaaat atggatatat ggattacttt ggagatgcaa taaattattt
     1141 aagtgaagta ttaaatatag atgaatactg caaaagtgaa tggagctatc tcgataatag
     1201 caaatctcca tctgagatat ttaatattct aaacaataaa atatttagta taaggctttc
     1261 taatgaaaaa agaaatgaaa ttttgacaaa tgataaaatt gaagcattaa aaatgaatta
     1321 tagatttgaa tgtattgatg aagaatatga tgaatatttt aaaaaatata agtctataat
     1381 caaaagaggt ggaaagaaaa aagttgaatt agtaaataaa aaaggtgaca ttttagaaat
     1441 atttaagtct ttagaagaat gtgccattta tttgcaaaag gaaaagaatg aagttataca
     1501 aatgttaaaa tatggagata caaaagtagg aagaaacttt ataaggtata gtttgagaag
     1561 tatttaattt cattttaaat tcataagagt ttcatttctg attcacacat ttaatattat
     1621 tttaatgtaa aataataaag gtgaagtatt gcacaattaa aattaaggag ggtattaatg
     1681 tctgaagaaa aaaatccaaa agaaaataca ttagatgaac ctaaaaattc agataatgta
     1741 aattctaaaa aagatgatgt gtcagatgaa aaaaatactg ataacaaagt taaaaaaagg
     1801 gaaataaagc taaacaacaa aacacttaaa gctattgtag caattgttgc agtaggagct
     1861 atatgtttag gtggaggcta tgtttttggc aaagaagctg gaagaaaatt gcctgctact
     1921 tcaaaacact ataatagcag tgaggtagta gctactattg gggataaaaa aataacaggt
     1981 gaaatgttaa gaaagagaat ggagccatta ttttatttaa atgcaaaaac tactatgact
     2041 gatgacgaaa tacgttcata tgaatcaaat atgattgaaa ctatgattac atctgatata
     2101 ttatcctctg aagcagcaaa agagaaaata agtataacta atgaccaact agatgctcag
     2161 tataataatt taatgagtag tatagaatca gcaatgggaa tgactaaaga agagttttta
     2221 aaacagtttg atttgactga aaaatatata aaagatgata tgaaaaaaga gctagtagca
     2281 tcaaaatatt taaatgaaaa atcaaaagtt actgataaag aagcagagaa ctattacaat
     2341 aaaaacaaga gcaattattt acaagttaga gcttcacata ttttaataaa aactgttgat
     2401 gataaaggta agcaagttag tagtagcaaa aaagctgaac ttaagaaaga agctgaagaa
     2461 atacttaaaa aagcccaagc tggggaagat tttgctactc tagctaaaaa atactctgaa
     2521 gatagtagtg cagaatcagg tggagattta ggtttctttg gtaaaggtca gatggtggaa
     2581 tcttttgaaa aagcagcctt tgcattgaag aagggagagg tatctaacaa acttgtagaa
     2641 agtgattatg gatatcatat aattaagaaa actgatgaaa aatatcagcc atttgaagag
     2701 gtaaaatcag atttagtaag cagtttaaca tcagaaaaac aaaacctact tatacaaaat
     2761 ctaaaagaaa aatataatgt taaaattaca gataaatatc aaatgaaagc taaagaatag
     2821 ataaagtcaa tttattaaaa aaaagaaaaa ttaactgtat ggataaaagc tatttttact
     2881 ctcaatatag agagtataaa atagctttta ttattttgca ataaaatcta tttatatata
     2941 tgaagtgaca aagaaataac atgcaaaaag atattaaaaa gaaatatttt taatttgaat
     3001 tttttcaata aaaatttaca aaaatgaaaa aataaacaaa aaagaattga agatttaagt
     3061 tgttatttaa atatatatat attaagattt tctgctgaag atactaaaaa aataatattt
     3121 ttcataattt taaaaataat aataaaaaaa tgaaaataca ggcgataatc tattgagtaa
     3181 acatagaaaa gatgatatta taagtatgtg ctaaaatggt taaagcaata ttaatattaa
     3241 atttaactat aaaacaaaag ggggcgtttt tttatgtcaa gtacagaaag cgtatctaaa
     3301 aaaagtggtc atccgaagag tttttggctt atttgcatga caattttgtg ggaaagattt
     3361 agttattatg gtttaacagc tttattggta ttatttttta cagcaagtgt agctcaaggt
     3421 ggtttaggtc tttctactca agaagcaaca tccatgtttg gcttatttac aggtcttata
     3481 tatcttacac cattagtcgg tggctggtta gcagataaag ttttaggtca acaaaaatgt
     3541 atttctatag gtgcactttt aatagcaata ggagatttcg tactatttgc ttcatctaca
     3601 tctattgcca tggtttgggt aggtctatca ataattattt taggaaatgg tttttttaaa
     3661 tcaagttgct caaatatagt tggggatata tatcctaaaa ctgatgtgtc aagaaaagat
     3721 gcagcataca gtttattcta tatggcagta aatgtaggag catttatagc tccaataata
     3781 tgtggattgc tttctgataa tgtatttgca gtaagaggag caagtggaga tatagtatct
     3841 tatggatata agatggcatt tttaatttgt ggtataggca tgatgttagg tatgattata
     3901 tttacacttc ttgcaccaaa attattaaat gaagtcggaa aatatcctgt gactaaaggt
     3961 aataaaggtg aaaaaattga gtatggacca cttactaagt tggagaaaaa taggataata
     4021 gcaatggtta ttttattttt atttgtggtc ttattctgga cagcatacta tcaaacacaa
     4081 agttctttta tgatttatgt aagggataat gtaaatagaa ctctatttgg atttgaaatg
     4141 ccagtagcat ggcttacatc actaaatgct atattatgtg tggctttatg cccagtatta
     4201 gctaccctat gggttaaatt atctcatacc aaaagaggtg atttctctat tccagttaaa
     4261 atgggattag gaatgataat aatgggtgtt gggtttatag ttatggtatt tgctacatta
     4321 gctaatggtc cagatggaat ggtaaaggct agtatattgt ttatagtatt gacttatata
     4381 tttacaacag taggtgaatt attcttatct cctataggac ttgcaatgtt caataaatta
     4441 gctccagcta agtatgctac gttgtctatg ggagcctggt atctaacaag tttttttgca
     4501 agtttattat caggaaagat tgctggtttt acagccacac ttggtttttt acagatattt
     4561 ggaggaatag caacagtttt aataatattt ggtttaatat tattgggaat aagaaatgta
     4621 ttaacaagat taatggctat ggatttacta acaaaagaaa aataattcat gacgaattat
     4681 tgtaaataaa tgaattactt taaataaagg aagttacatt taaatataaa attaaatgta
     4741 atttcctctt tgattatgaa aaaaagcttt acacaaatag atattataat taaaattata
     4801 attgtgataa ttagagtgta taagatttat ataaattata caaaaaatat ttatgtaata
     4861 aactttacat acttatatat ttagttattc aaactattat ttcgaggagg tcttgttgtg
     4921 aatataaaag atagattaag tggattaaga aaatttatgg aagaaaaaaa tatagatgcc
     4981 tatatgattc catcttctga taatcaccaa agtgaatatg taggagatta cttcaaatca
     5041 agagaattta tatcagggtt taatggttca gcagggacgg ttattgtgac taaagacgaa
     5101 gctgggttat ggacagatgg aagatatttt atacaggcag aatctcaatt agaaggaagt
     5161 acaataaaat tattcaaaat gggacaagaa ggttgcccaa ctacagatga atatttatat
     5221 aaaaatattc ctgaaggagg aactttagga tttgatggta gagttatatc tgctagagaa
     5281 ggagctacat tagctgaaaa attatctaaa aaaggtataa agatagaata tcagtatgat
     5341 ttaatagatg gtatatggcc agatagacct gctttatcag atagtaaagc atttttatta
     5401 gatgttaaat attgtggaga aagttttagc tcaaaattag ctagattaag agaaaaaatg
     5461 agtgaaaaag gtacttctac tcatgtaata actactcttg atgatatagc atggttattc
     5521 aatataagag gtggagatgt taaatataat ccagttgtac tttcttatgc agtaataact
     5581 ttaaaagaag tttatctatt tgttgatgaa agtaagttaa atgaagaaat actaaatgaa
     5641 ctagctaagg aaaatgttca aataaagcca tataatgatg tgtatgaatt tgtaaaaaat
     5701 atagacaaaa ctgaaaaagt tttattagat ggaactaagc ttagttatac aatttacaat
     5761 aatatacctt gtgaagttga gaaagttgat gagttcaatc cagtaatgtt ttttaaagct
     5821 caaaaaaatg aggtagaatt agaaaatata agaaattcac atgtaaaaga tggtgtagct
     5881 ttcactaaat ttatgtactg gttaaagaaa aatgtaggta agatggaaat cactgaaata
     5941 agtgctactc aaaaattaga ggacttaaga agagagcaag aaggtttctt tgagccaagt
     6001 ttcaatacaa ttgctgcata taaagagcat gctgcaatga tgcactatag tgcaactcca
     6061 gagtcaaatt ataaattaga ggcagaagga ttatttttag tagattcagg tggacaatat
     6121 tatgatggaa ctactgatat aactaggact acagttttag gacctataag tgatgaatta
     6181 aaactacact ttacttctgt agcaagagga atgataaatc tttctaaggc taagttcttg
     6241 catggttgta gaggatataa cttagacata ctatctagaa gttgcatgtg gaatatggga
     6301 attgattatc aatgtggaac tggacatggt ataggatttg tgctaaatgt tcacgaagct
     6361 ccaaatggat ttagatggag agtagtacct gaaagatttg atagtgcagt tcttgaagaa
     6421 ggaatggtaa ctactaatga accaggtata tatatagaag gttctcatgg tataagaaca
     6481 gagaatgaaa ttgtagttag aaaagctgaa aagaatttct atggtcaatt tatggaattt
     6541 gaagttgtta cgttagctcc tattgactta gatggtatag taccagaatt aatgaataag
     6601 gatgaaaaag attatttaaa ctggtatcat aagttagttt atgataaaat atcacctttc
     6661 ttaactgatg aagaaagaga atggttaaaa gtttacacta gagctatata gtttgtacta
     6721 gatacaataa aaatcgaggt attataatgc ctcgattttt tattgtatat tttattaact
     6781 ttaaaaaatt tttaatttga taattctact aattttttat tcattataag tagtataatt
     6841 ccaaatatca ataaaaatga tggtatcact atgaagactt gtaacatacc aagttggtct
     6901 ataatagctt gcgtatagcc agataattta tttgctgcaa atttgcctaa aaaccaaaca
     6961 cccattagga atgaaatata cttttcaggc gaaagtttat ttaccatacc atacccgact
     7021 ggagaaaagc aaatttctcc tatactttgg aatgttaaaa atccaactac ccaaaataca
     7081 cttgctttgt gaattgaatt agggtctgtt cctgtttgta taactgctac tatcataaac
     7141 aaaaatgcta tggctaatag tataaaacct aagctcattt tttttggcac acttaaatcg
     7201 ccttttttag tttgagataa ttttacccat actaaagcag agataggtcc aagaataaca
     7261 caaaggagac cattgtatga atctatccat gaagtaggaa tttgaaaatt gccaacagta
     7321 agatttatgt gttcctttgt atagagtgca atagacatag aggcttggtt atatgctatc
     7381 caaaaaaata ctgtgaaaaa agctaacacc aatattacta ttattctttt tttctcaatc
     7441 aaagttaaag gtcttgaagc tagcattttc ttatattcta gtgattttct tatagactct
     7501 ctcccgttac tagggtcatt attcaaaggt ttataaaact ttctgtttcc aaatataaag
     7561 aagaataaac ctattagaac cgtaattgat gaaaataaaa atacatatct atatccatac
     7621 atacttatag ataatccgct tttcatagca aaccattcat tagcaactaa accgcatatt
     7681 aaagggccaa ataaggtacc tagattagta gccatatacg taatagagaa agctccatct
     7741 tttcttgggt catcattggt atataagttt ccaaccatag tatgtaagtt tcctttataa
     7801 aaacctattc ctaatccaga taaaaatatc atgatgttta tatccatctt actatttgct
     7861 atgtatccta gaaaataacc aattgaaacc attagcattc caagtataat acagtttttg
     7921 ggacctaaaa atctatcact gacccatcca ccaataactg gagagaaata agtccatgct
     7981 aaaaaagatg caagcattga tgcggattct gctggtgtta accctaaacc acctttggag
     8041 acttgttcag ctaaaaacaa taataatacc aatttcatac catagtatgc aagtcctaca
     8101 aaggtaaata ttgaacagca gaaatataaa tctctaggtt gtttaagttt ttcttgcgtt
     8161 tttaaaactg acatatgtcg agaacctcca gataaatagt ttaacaaaat tttaccatac
     8221 tatatgagta tatgtttttt tcatatagac gtcaacttgt aagataattt aattaagtcg
     8281 aaatatatgt aaaaatagta tcatttttgt gtattcagta atattttttt atgataatat
     8341 atgttattat tatattaaaa tacaaacata tttatggaaa ggatatgcca atttagtagt
     8401 tagattgatt gtttggcagg tgggatgagc tatgataaga aagaaatctg aattacaagt
     8461 agaagtaaaa gaaaatttaa atggtggtat aggaaaggtt aagcttgaaa acataataca
     8521 aaatgaagag cttaaaggaa aaggaagact atttaaaaga gtagctttac cagtaggaag
     8581 ttcaattggt gtgcatgacc atacaacaga ttttgaagta tattatatac taaagggaaa
     8641 aggtaaagta tttgataatg gggaatttgt agaagtaaat gagggtgatg tagtgtatac
     8701 agctgatggc gaaaaacatt ctatagaaaa tataggagaa gaagatcttg agtttgtagc
     8761 actagtttta tatatgtaaa atatggagga ggagtcatga caggcaagat agaagaaatt
     8821 aaggacaaga taaatagatt ttgcaactcc aatttaaatc aagaatacaa agatataagc
     8881 tttaaaattt tgcaaaacct tttagataat aataaagaga taattcattc ttctagagct
     8941 gatatatggt cttcagctat actaaatata gttttagaac aaaatctttt atacaataag
     9001 aagcatccac tacatataac aaaaaaagag ttttcaaaac aaataggtgt ttctttaaat
     9061 actataaata ataaatcgtc tttaataaaa gatgtttgta gtattgactt tttaaatcaa
     9121 gatactatag ctatttctaa tgtagaattt tgggtaaaag agaccaattc caaatcaaag
     9181 tctgtagatt tagatttaga aaagaaaaaa atactatata aaagatatat taagctatca
     9241 caagatgcga atagtgatat tgaatcaatt agatatatgg aggaagctgt aaatataggt
     9301 aaaagtatga ttactaaaga agataaagaa cttggatttt ggaaaggttt atctaccaga
     9361 gcctatatga tagcacttga atcccttgct agaaagttag aaagtattaa taatctaaag
     9421 gagtccaaaa aagtattaga atatttaata gaaattaatc ctagtgatga gcagggaata
     9481 agatacaaat tattaaatgt attaattaga ttaaatgata gaacttcaat aaataaatta
     9541 tttgaacttt ataaagaaga aaaatctgct acatggatgt acagtaaagc tctttattac
     9601 tttaaaaata aaagtatgtt tttggcaaat gattctatta aggttgctag aagtaaaaat
     9661 aagtacgttg gattatattt gttagattgg cgaaatgctt ttggtagaga atttgttaca
     9721 gaggaagaaa aggcagaagc tgtctattac tatgatgaaa acatagtgat ttggaatgaa
     9781 gttaaaggtt ctatggattg gctattaaaa aaaatgttgg aattttcata aaaaaaacta
     9841 ctccttactt ttatataagt aaaaggagta gtttttaatt tatacattta gttttaaatc
     9901 ttcaggtttt attagacctt tttttctcat aaattctgaa atcaacaaag ttacaatagc
     9961 aggaagtata aaattaagta aaagtatacc aacccacata gatatacctc cttttccagc
    10021 actttgcata gcagttactg ttccaaattg tccaaccaat ccacaagtac ccataccaga
    10081 accaatagga atattctcca ttttgaatat tgttgtagat aatggtccaa gtaatattga
    10141 agctacagta ggtggtatcc aaattttcca attttttatt atattaggta cttgcaacat
    10201 agatgtccca agaccttgtg ccacaaggcc accaactcca ttttccctaa agctcataac
    10261 agcaaagcct accatttggc aacaacaacc tacagttgct gccccaccag caagaccacc
    10321 taaacttaac atcatacaaa tagctgcact acttataggt aatgtaagag ctattccaac
    10381 aagtgcagag actaaagctc ccattattat aggttgcata gttgtagctt gcattattat
    10441 atttccaaat gaagtcataa aagcagaaac agctggacca ataagttcag ctacaagtac
    10501 tccaattagt attgtcacgg caggtgtaac caatatatct atttttgttt cttttgaaac
    10561 taattttcca acctctacag caaaaagtgt agcaacaaat gctccaacag gtcctcctaa
    10621 ttcattacca caagctccaa caagtgcaca agaaaatagt acaagtggag gtgcttgaag
    10681 tccatacgca atagccactg ctatagctgg accagtaacc tgtttagcaa gagggcttat
    10741 aacttcagta aaaagagaaa tattgaaatt atctcctaat gtattgaaaa ttgttccaat
    10801 taaaagggac gaaaaaagac cgagtgccat ataacttagt gcgtcaataa gatacctttg
    10861 aacagaaatt tcaatatttt ttcttttagc aaatgatttg aaatttcttt ttttataatt
    10921 gatttccaat ttaaaatcct cctcatataa tataagtata atttcttttt aattaattat
    10981 ataacttttc agctgacaag acaaatgtta tttcactaaa attagactat tgtaatagta
    11041 agaaattaac attaatttta gttttaaatt ttaaatttat ggtttataat tagtataggg
    11101 atattctgaa atataataat tttccagttt atcccaatat ttaacttagg tagattgctt
    11161 acaaaacaaa tgattacata ttaggctaaa aggaagtgga tagatgatat ttgtaaaaaa
    11221 taagaaatac agaaaaattg ccataattat atctattcct atattgcttg ttgcgcagta
    11281 ctaccttttt aaatttggaa tacttagacc aatggtaaag ggagtagaaa ttgaaattgt
    11341 agatggggaa tatgttaaag atatagataa atatgtagta aaattaaatg aaacagttac
    11401 tttgtctaca ggtgaatata ttaaggttcc ttcgtatgct aaaaatccaa atatttggtt
    11461 taatgtattg gataactcag ggactttaaa aatagatgga aataaaatga cagcattaaa
    11521 ggttgggtat tcttctgtgg gtatcatgaa aaattcaaga gtattaaaaa aggctactat
    11581 aaaagtagta gacccagagg tagaatcact agatatggag attaatgggg atttaaaaca
    11641 tgttggtgat agtgctacta tagaaagtac aatagaagtt gattatgata agtttaaaaa
    11701 atcatataag ccagtttata agagtagcaa tgaaaatatt ttaaaaatca aaggaaataa
    11761 agtaagtgca gttggagttg gtaaagctac aatatatgct aagtgtggaa ataaagaaat
    11821 tgagagaaca tttaggattc aagctagagt tgctgaaata aatatggatg atataaaaat
    11881 agaagttgac cagaatgaaa aattgaaacc agatataata acttctccac ctggtttaga
    11941 accaccaaaa atatattatg agttagtaga ttcaaaatta cctgtagaaa gagcaataag
    12001 tttaagtagt aatggaaatg ttgtagggct tagagaaggt aaagataggg tgagaataac
    12061 ttgtggagat aaatctaaag taataacagt aacagtagtc aaagaaacta taacgaatga
    12121 ctatatacaa aacttggttg gttcacataa aatagtaaac aataaagtta ttattaaact
    12181 tgaatgggat tatatgaaag atgtctttga ttatgatata tatcttaaag ataagttgtc
    12241 aggagaatca tttaagaaaa ttaaatctat aactataaat gaaagtgatt taggtaagtc
    12301 taacaaggta tatgcaacta tagaagtaga tatgaagggt aatcaaaata tagattttga
    12361 tatttatgtc atagggaaaa aggattcaga tacaactaag ccaagtaatg taataaatat
    12421 taccaatgga aatggaaatt caaataatga taataatatt aaagatatga gggtagaaaa
    12481 tatatcagct aatgttgata gagataataa tgtcattaga gttaactgga gtagtatatc
    12541 tcataaggac tgtacttaca gtatatatgt aaaaaataat actaatggag atggagaatt
    12601 tactctatat caaagtgata tacagggaaa tgagcatact ataagtattc ctgaaggaga
    12661 tttaaatcta gatatttatg ttgttgcaaa ttataatgga aatagctctc aaaatagtga
    12721 tgttataaat gtaagataga tttgtataca aattgaaagt gttgagttta tttaaatatt
    12781 aaattaagaa aaaggatagt atagggtaat tatactatcc tttttttgtt ataatatata
    12841 ggtttaaaat ctctagtaat ataaattata aaaagtctta tttacacaaa tctatagata
    12901 ttctttttat taatcaatat tgtcttaaat tgaatttagt gcagcttcta ttgcatgtat
    12961 tactgtcgta tcaaatagtg gaatagaagt gtcattttgt ttaattaaaa gacctatttc
    13021 tgtacatcct aatataacag cctctgcacc ttgtttgcca agcttatcta taatagataa
    13081 gaaagctttt tttgaagatt cagatataat tcctaagcaa agttcattaa atattgtgtc
    13141 atttactatt atacggtctt cttcatttgg aattaagact tcaattccat tattaataat
    13201 tacattttta taaaaatctt gttccattgt atatttagta cctaataagg ccactttttt
    13261 tatttctttt tcccttaata ccgtagcagt tacatctgct atgtgaagta atggaatatg
    13321 tacactttct tgtattttat ctgatacctt atgcatagta ttagtacaga taacaataaa
    13381 atctgcccct gcctcttgta atttaattgc agcatcagct aaaattttgg cgcttttttc
    13441 ccaatttcca ctagattggc attcttcaat ttcttgaaaa tctacactgt aaagtataca
    13501 tttagaagag tgtagtccac ccaatctttc ttttattact gtattaataa cttgataata
    13561 tgtaatggta ctttcccaac tcataccacc aattaagcct atagtcttca tattatacct
    13621 ctcttattta tttattttga aatagctatt tacaaataca agtatagcat ctaaaataga
    13681 agcatacaat aataaaattg tatggagtac aattataatt aagaggagta attatatgcc
    13741 aataaattct tttgaaaact atcctatgac atggaaacca aaattagata accgaaaacc
    13801 accaatatat aaaactttag caatgttatt agaagaagat attaaaagag gaaatttaaa
    13861 tcctggagat aaactgcctc cacaacggga attagcagat tttctagatt taaacttaag
    13921 tacaataaca cgagcattta aactttgcga agaaaaggga ctaatatgtg caaaagtggg
    13981 aaagggaaca ttcatttctt ctgatgtaaa tgtaagtaat acattattat atcaaacgga
    14041 atcaaaagac ataattgaat tagggacagt tcatcctcca tatgagcaaa atacatatat
    14101 tattgacttt attaaaaatg tactaaaaca accagaaatg gataggtttt tacgatacat
    14161 gtctccatct ggcacatata tgcaaaaaaa gtcaattgca aaatggatag aaagaaataa
    14221 tgtatacacc agtgaagaaa atatattatt atctacagga gggcaaaatg ctatatgtgc
    14281 aactttacta ggattattta aagctggtga tagaattgct acagattctt taagtttttc
    14341 tggaattaag tcaatagcta aaatgatagg tattcaatta gtcccaattc ctcaagaaaa
    14401 taatgaaata tcgattgaat atcttgaaag ttattgtaaa aatgaaaata taaaaggcgt
    14461 atatcttata ccagattatc ataatccaac gacacatact atgtctgatt tatctcgtaa
    14521 aaagatagcc aagatagcaa agcaatataa tataataatt attgaagatg ctataaatag
    14581 catttttaga gatggtatac aaactcccat attttcttta gctagtgata atactatata
    14641 tatttttagt acatctaaat ttttatgtgc tggtcttaga gtagcatttg tagttgctcc
    14701 aaaacgatat atagaaaatt tagaaaatgc actatataat atgaatctaa tggtttctcc
    14761 atttacatca gaaatagtac accgtttatt atattctcct attatagata aaattataga
    14821 agagaaaaga gatgcaatta tagaaagaaa tgaagttgct gataaagttt tgtcagatta
    14881 taatttgatt ggtgataaga attgcagttt tcgttggtta cttcttcctg atgaattgga
    14941 tggaaaatca tttgaaattt ctgcaaaaaa tctaggagtt caagtatatt gtgctgaacg
    15001 attttcagta ggaaactcta cagttccaaa agcagttcgt atttgtgtaa cagctccaaa
    15061 agatgtagaa gaattagaaa aagggcttaa tataataaag tcacttctta agagttaaat
    15121 ttattttact attatttaat taaataaata aaaaggcgga gaaatttatt ataataaacc
    15181 tccgctttta ttatttaatg aaatacttta ctttttatta aaaaatttaa ttttaataat
    15241 cttgaaaata taatcgtttt atggtaaaat aattcttaaa aatagaaaaa attaatcatt
    15301 tgttaaaaaa aatgcataat ttattgtaat ttattgacga aggttgaaaa atattcataa
    15361 atgtgcgaaa taacagaaaa aatgcaaaaa ttaattcaat ttcatttgtt ataaaatcta
    15421 tatggcaaaa ttaaatctgc ctatcagaat gatataaaca catcagaaag gaggacgcta
    15481 tcaaataata caagtagtta actttgatag caaaaaatta tgaatgaaca aactagaata
    15541 tccttagaga gagctgctga attaaaatca aaaattgatg attatattca ggctagaaaa
    15601 acgattaaca gaggtcttga aaaagaagaa gagataaata aacgaaaaca gaaaatatta
    15661 agtatcttaa atggaactga agaggattgg aataactaca aatggcaatt atcaaataga
    15721 ataacagatg tagatacttt atcaaaaatt ataactctaa ctaaaaaaga aaaagaatat
    15781 ataaaagagg ttggtactca atttagatgg gcaatatctc catattattt gagtcttata
    15841 gacccagaag atatatgtga cccaataaaa ttactgtcta taccaacaca tatagagttg
    15901 gaagatgaac aagaagattt ggacccaatg ggagaagagt atacaaaccc agcaggatgt
    15961 ataactagaa gatacccgga tagattaata ataaatgtaa caaatgagtg tgctatgtat
    16021 tgtagacact gtcagagaag aagaaatatt ggacaacaag attctcataa gtcaaaagct
    16081 attatccaag aatctataga ctatatcaga gaaaatgaag aaataagaga tgtactagta
    16141 actggtggag atgctcttac attaaaagat gattatttag agtggattct tagccaactt
    16201 aaagagatac cacatgttga ttatgttaga ttaggtacta ggactcttgt tacaatgcca
    16261 caaagaatta cagatgaatt ttgcaatatg ctaaaaaaat atcaccctgt atatataaat
    16321 actcatttta atcatccaat ggaaataact aaggaatcta aagaagcttg tgaaaagtta
    16381 gcaaatgcag gcgttccatt aggaaatcag gcagtattat taaatggaat aaataatgat
    16441 aaatttgtaa tgagatgttt aaatcaagaa ttactgaaaa taagagtaaa accttattat
    16501 atattccaaa gtaaacatgt aaagggaaca aaacatttca atacatcagt agatgatggt
    16561 cttgaaatca tggagtattt aagaggatat acatcaggaa tggctatacc aacatatata
    16621 gtaaatgctc caaaaggagg aggaaagact cctttgcttc ctcaatacct tgtatcaaaa
    16681 ggaacagatt acgttatgct tagaacatgg gaaggaaaag ttataaaaat ggaagatgaa
    16741 cctgctgtag atataaagaa acttataaaa gaacaagcac aggattaaaa aaatatttaa
    16801 agtttataaa aagaaacaac tttaaaataa aaagaaaatg gatgtcaaat ttatatcaat
    16861 ttgcatccat tttttattag ttatttatta ttttgttgtt gattaactaa ttcaagatat
    16921 ccacttgggt ggaagtaata tatctcagaa gtattttcat ttacaaagta accaatatca
    16981 ccatctgcat ctggtaaaaa tacataacca ttaagatttt tagcttttaa tgcttcaaaa
    17041 tcactagcat ttccttgata tatgtaatca acttcattgt tgtattttac taaaagttct
    17101 ttagcttgtt cttgagtcaa tttagtattt tggcttacag gaacattctt ttgagaatct
    17161 gcataagaag attcatttac tggtgcagtt aataacaaac aagataaaac taataaagat
    17221 ataagtgctt ttttcatact aaaaccctct ttcatgttta attatataag attcattact
    17281 taataatatt gtatgtttcc tataattaaa tattaccatt aaagagaata atattacagt
    17341 tacacagctg taacttttga ttacaaaatc gtaattaaat tgtattttta ttccatttgt
    17401 attttatcat gtctacgatt tacaaactca cagaagtata tctttataac gcccatcaca
    17461 ggagcagata gaatcatacc aggtatacca aagaatcctc caccaacagt gactgctagg
    17521 attgtaaaga atgggctaag acctagttgt ccacctacga tttttggctc tataaatgta
    17581 acttcaactt gttgaattat tattaagtaa attaaagaag ttaaagcaat tgttggattg
    17641 gagaataaat taattattac aactggtgcc atacctatta ctggaccaaa atatggaacc
    17701 atattcatga cacccataag tgtaccaaat aatagtgcat attcagattt taaaaagtaa
    17761 agaccaattc cagaaagtac tccaactata aaagaatcta aaatcttacc tgtaaagtat
    17821 ttaccaatat ttaaatttaa gctttgacat agttctatta taaagtctcc atatttttta
    17881 cctaaggaaa tataaactac tttttttgta aacagtaaga atttttcttt ttctaataat
    17941 atatagaaac aaattataaa acctaaaaga aattgtacta taaattttcc aactgaaaat
    18001 gtagtgctaa atatcccacc aagtgaacca ataagcaagt tactaaattt aggcattaca
    18061 ctcataattt tgtcaccata ctcttttaat gtattagggt caactgattg taaactctta
    18121 cctaagtcga ataagaattg ctctgtttta tctatgtatg taggaatttg agcaactaaa
    18181 tcagctaaac tatttactat ggatggaact gtaaataata taaaggaaat tagtataact
    18241 atgagtacac cataagtaag aagtaacgaa tatattctct taaaatttaa tttactttct
    18301 aagaacttta ctagtgggtt gaatatatat gctaatacaa atgctattat aaatggtgtt
    18361 agtagtgaca ttaataaact tatcactcca aaaaaatatt tgtaattatc aataacttta
    18421 atcaagatat atgaaacaac tacagtgagt acaatattta gataaatttt tttatcttgc
    18481 aaaataacac aacctttctt atatttatta aatttagata tacttaaatc taagttaaag
    18541 ttaatattta tag
//
LOCUS       DAEMHZ010000074        18344 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000074 DAEMHZ010000000
VERSION     DAEMHZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.074"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(201..917)
                     /gene="dapD"
                     /locus_tag="KPK72_002347"
     CDS             complement(201..917)
                     /gene="dapD"
                     /locus_tag="KPK72_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG2489555.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1141..1890)
                     /locus_tag="KPK72_002348"
     CDS             complement(1141..1890)
                     /locus_tag="KPK72_002348"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2489556.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(1957..2844)
                     /locus_tag="KPK72_002349"
     CDS             complement(1957..2844)
                     /locus_tag="KPK72_002349"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2489557.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(2961..3962)
                     /locus_tag="KPK72_002350"
     CDS             complement(2961..3962)
                     /locus_tag="KPK72_002350"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG2489558.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4387..5268)
                     /locus_tag="KPK72_002351"
     CDS             complement(4387..5268)
                     /locus_tag="KPK72_002351"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2489559.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5591..6784
                     /locus_tag="KPK72_002352"
     CDS             5591..6784
                     /locus_tag="KPK72_002352"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG2489560.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            6990..8108
                     /locus_tag="KPK72_002353"
     CDS             6990..8108
                     /locus_tag="KPK72_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG2489561.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8239..8484
                     /locus_tag="KPK72_002354"
     CDS             8239..8484
                     /locus_tag="KPK72_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG2489562.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8471..9217
                     /locus_tag="KPK72_002355"
     CDS             8471..9217
                     /locus_tag="KPK72_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2489563.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            9500..10375
                     /gene="hslO"
                     /locus_tag="KPK72_002356"
     CDS             9500..10375
                     /gene="hslO"
                     /locus_tag="KPK72_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG2489564.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEDTIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            10766..11566
                     /locus_tag="KPK72_002357"
     CDS             10766..11566
                     /locus_tag="KPK72_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2489565.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            12093..14333
                     /locus_tag="KPK72_002358"
     CDS             12093..14333
                     /locus_tag="KPK72_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2489566.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            14747..16282
                     /locus_tag="KPK72_002359"
     CDS             14747..16282
                     /locus_tag="KPK72_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2489567.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            16298..17059
                     /locus_tag="KPK72_002360"
     CDS             16298..17059
                     /locus_tag="KPK72_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489568.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            17397..18266
                     /locus_tag="KPK72_002361"
     CDS             17397..18266
                     /locus_tag="KPK72_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2489569.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIDLISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
BASE COUNT         6832 a         2278 c         3113 g         6121 t
ORIGIN      
        1 aaatacttgt cagcaagaaa taaaaatgat ataagaatga ataagagaat tgtaaaaaaa
       61 taaaatgatt ataaataaaa gataaaaata ttgtaaaaaa gagggtgcct ctggtgaatt
      121 aaattcacac agagacacct ttttaattaa ttttttaaat tagtatatta ttgtttaatt
      181 aatagtatag tactatttaa ttagtctaaa tttctaaggt cttccataag cttagttttg
      241 tcagctgttt tttcatcttt catttttata actttagcag gagaaccagc aacaacagca
      301 ccagcttcta catcagtagt tacaacagca ccagctgcaa caacagcacc tttaccgatt
      361 ctaacacctt caagaataac tgcattagct cctattaaaa catcatcttc aactataaca
      421 ggagtagcag aaggtggctc taaaacacca gcaacaacag caccagcacc taagtgaaca
      481 tttttaccaa gagttcctct agctccaata acagcattca tatctaccat agaaccttca
      541 ccaataacag ctcctatatt tataacagct cccatcatta caacagcatt tttttctata
      601 gtaaccatgt ctcttattat tgcacctggc tcaattcttg catgttcact taaagtattt
      661 tttagaggaa tagcagaatt tcttctatca tattctacat gagtatctgt tatatcattt
      721 ccatgtttat ctaatacttc cattatatcc tcatagtcac ctacaaatat ataagaacca
      781 ttatttccaa ataccttaaa tttatcagtt gattggaagt gtaaagagtt agtatttaca
      841 tataccttaa caggtgtttt ctttttaact tcttttatat accttgctat ttcataagca
      901 tcgtttaaat ttaccatttg ttaattctcc ttgtatttca ttatatttct tttattatat
      961 acttatttgt caattttagt atatacttac ttcttattat ttatatttta ttttagttag
     1021 tttttattgt atagacatag tattaaataa atttattata tatgtttgat ttgtattgcg
     1081 tattattata tctatattgt atatgtttaa tttatattgt gtattattat atttatacta
     1141 ttatcccaac atgctgtcca tattataaaa tcctggtttt tgcttaacta agtatttggc
     1201 agcagctata gaaccacttg caaagatacc acgagattga gcttgatggc ttattgtaag
     1261 aacctcattg tctccagcaa atagagcatc atgttcgcca acaatatttc cacctcttat
     1321 agcatgaata ccaacttcat ttggttgtct ttttgcacta cgaccatatc taccataatt
     1381 attttcaacg tttggcaaaa cctcttttat agcgttagca atcataacag ctgtgccact
     1441 tggggcatct acttttctgt tgtgatgttt ttctattatt tctatatcaa atccttctag
     1501 taatttagct gcatctttaa ccaattttac taatacattt acaccaagag acatattata
     1561 tgattggaat acaggaataa ctttagccgc ttcacgtatt ttgttcattt cgtcatcatt
     1621 atatccagta gtagcaatta ctaaaggtgt tttagtcttt aaaacgtaag ataagatatt
     1681 atctaaatta gagtgatgag aaaaatctat aactacatca gcttcctcaa ctatagtact
     1741 catatcttca taaattttaa ggtcgccttc atagctatcc agacctctag ctgcaacaca
     1801 aaccaattct aattcattat cttctcttat agtatcggct aaaatcttac ccatttttcc
     1861 atcataacca gttgctataa cttttaacat aatttcctcc tatgattaag ggctaacttt
     1921 tgaccaaact tactttttac catgcattaa tctttactat gcattaactt ttagtccaaa
     1981 gttaactaat tctttcttta acacttctaa atttgctggg tccatttctg ctagaggtaa
     2041 tcttaaatct ccaacattga atcccaataa attcattgca gtcttaactg gtataggatt
     2101 tacttctata aataaagctg cgattagagc atccatacca agttgtaatt ttctagaacc
     2161 ttcaatatcg ccttcaaaga atttagctac caaatcgtga gtttcttgag ggcaaacatt
     2221 agcaagtact gatataactc cttgacctcc taaagataat aaaggaagta tagtatcatc
     2281 attaccagag tagatagcaa agtcatctgg aactaaacta gctatttctg caacctgtcc
     2341 taggtctcca ctagcttctt ttacagcaac tatgttatca attttagcaa gttcaactat
     2401 aaggctagga cttatattta cacaagttct tccagggaca ttgtaaagta taattggaag
     2461 tttaacactg ttagctattg tttcaaaatg acgcttaagt ccagctttat tagctttatt
     2521 gtaataaggt gttattatta atagagcatc tactcctaat ttttcagctt cttgacttaa
     2581 gttaactgag tgcatagtat cattagaacc acttccagct ataacaggaa ctcttttatt
     2641 aactttttca acaacatact taatagtagc aagttgttct tcatcagtca tagtattagc
     2701 ttcaccagta gtaccacaag atactatagc atcagtacca ttagctattt ggtattcaac
     2761 taattcacct aatttgtcaa aatcaacttt gttatccttt gtaaatggtg taactagagc
     2821 aacagcagaa cctttaaata tcatataaaa tcaatcccct cttttttatt tttgtgtttt
     2881 atattacagt tacttctaat gcttgttttt gagttactat taatatttta caaaattata
     2941 tatttataca agtttgtata ttatactaaa tcgtatgata aagccatttc agctatttga
     3001 attgcgttag ttgcagcacc ttttcttata ttatcagcaa caacccaaag atttacacca
     3061 ttatcaacac taaaatctct tcttattctt ccaacaaata cttcatctct atcatcaaat
     3121 tctacagcag ttggatattc gtttttagca ggattatcaa ctactacaac acctggagct
     3181 tcttcaagtg tttttactaa atcatctaac tcaaatggat tgttaaactc aacatttata
     3241 gattcactat gaccattttt tactggaact cttacagtag ttgcagttat ttttaaatcg
     3301 tagttatcaa gtattttcat agtttcattt accattttca tttcttcttt agtgtatcca
     3361 ttttcagtga atgaatctat atgaggaaga cagttgtaag aaatttgatg agggtagaat
     3421 ccagtaggat taccgttcat tcctttctct aaatcttcaa cacctttaac accagaacca
     3481 gatacagctt ggtaagtaga gtaaacaact ctcttaattt tgtatttatc atcaagagct
     3541 ttaaggggaa ccatagcttg tatagtagag cagttaggat tagctattat acccttatga
     3601 ttttttatgg cttctggatt aacctcagga actactaaag gcacatctgg attcattctc
     3661 cattgactag agttatctac tactaagatt ccatttgaag cagctattgg agcatacttt
     3721 tcactaatac ttccaccagc agagaataat gctatttgta tgcctctatc agtaaatgaa
     3781 gtttccttta attcttctac tatatattct ttaccacaaa acattacttt agaaccagct
     3841 gatttagaag aagagaataa aaatagattt tctattggaa aatttctttc ttctaatact
     3901 tttaaaaatg ttctacctac cattccagta gcaccaacta ttgctacatt tacttttttc
     3961 atataaattc accccaattt taaattatta aaacattatt ttattttagt tatacaagta
     4021 agatatgtta atatttttat atatccaaaa actttaatat tatattaaca actttaatta
     4081 tatacaaaaa acgtacaaag gtatactttg tacgtctaat ttatcactca aagtaaaccc
     4141 tgtagctcat cacctacaaa aataagtgac agtattacac atgttatgtg caatcccaga
     4201 aaatattatt gcaagaaata ttttcttcgg ctgtaaatcc tttttccaat cattaacacc
     4261 tgccagctac tattggatac tcttagttac agctacctct accctaggtt aataggatat
     4321 taaaatttga ttattctaat aataatacat atttaagaaa aaatcaactt atttatataa
     4381 atattgctaa aatttaaatc caaaatttgt caattcttgt tttaacacat taagattagt
     4441 ttcttccatt tcagctagag gaagtcttaa atcaccaaca ttaaaaccta aaatgttcat
     4501 ggcagtttta acaggaactg gatttacttc tataaataag gcagcaatta aagcatccat
     4561 atcaagttgt aatttttttg agccttcaat atcaccttca aagaatttag ttactaaatc
     4621 atgtgtttct ttaggacata tgttagcaag tacagagata acaccacttc cacctaatga
     4681 taatagtggt agtatagtat catcattacc ggagtatata gcaaagtctt taggtactaa
     4741 tttagctatt tctgcaactt gagctaagtc tccacttgct tccttcacag caactatatt
     4801 atctatttta gcaagttcag ccacaacaga tggctttata ttcactttag ttcttcctgg
     4861 tacgttgtat aaaataattg gcaacttaac gctatttgct attgtttcaa agtgaagttt
     4921 aagacctttc tcattagtct tattatagta aggagttatt attaaaagac catccacccc
     4981 taatttttca gcttcttgac tcatatgaac agacaacata gtattgtttg aaccagtacc
     5041 tgctataaca ggtattctct tattaacttt ttcaactgta tattttatta cagcaaaaat
     5101 ttcagattca ctcatagtag tcgcctcacc tgttgttcca caaacaacta gtgcatctgt
     5161 gccattctca atgtgatatt cgattaattc acctaatttt tcaaagttga cattattatc
     5221 ctctgtaaat ggggtaacta aagcaacagc agaaccttta aataacatat aatcaaatct
     5281 ccttttttat ctaaatttaa ttttttaagt acaataataa ttatagtaca aattaattaa
     5341 ttttaatagg tatagttaat atcttaactg tttaaataag atatttatat tatattaata
     5401 ttagaaaaat tttaaataaa tgttttattc tcaaaatatg catattaatt taataaaata
     5461 taccaaaatg gaaataaaaa ataaatttta tgagaattta ataactaata attaatatat
     5521 ttaggttata ataaatatgt gcgtaaaatc taacaataag caataatacc gtaaatttag
     5581 gaggaataat atggatactt taaaggaact ttttaaaata ggaagtggac cttcaagttc
     5641 tcatactatg ggaccacaaa gagcagcaga gagatttaag aatgaaaacc cagatgcaga
     5701 aagttttaga gcaatattat atggtagttt ggcagctaca gggaaaggtc atttaactga
     5761 ctatataatt gaaaaaacta tagcacctaa aaaagtcgaa attgtttggg aagaagatat
     5821 tataaaagat tttcacccaa atgggatgaa gtttgaagct ttagataaag ataaaaatgt
     5881 aacagctgaa tggactgtat attctgtagg tggagggact atagcagaag aaggtcaaag
     5941 aaacagtaaa agcaatagta tatatcactt agatactatg gatgagatag taaaatggtg
     6001 caaagaaaat aataagactt tggtagattt tgtgttggaa tgtgaaccaa aagacataaa
     6061 agattatata aaaactataa aagatgctat gagaaaatct attgatgatg gactaagtac
     6121 agatgaaata ataccaggca agttattatt aaaaagaaga gcaagtacat tttataatgc
     6181 atataaaaaa gataagagct tctcaacact tgtatatgca tatgcactag cagcttcaga
     6241 gcaaaacgct tctggtaata taatagttac agcaccaact tgtggttctg ctggagtaat
     6301 accaggaata ttctttgcaa tgcaggattt ctataattat gatgacgaaa aaataattga
     6361 agcactactt gtagctggaa taataggtaa tataataaaa actaacgctt caatatctgg
     6421 agcagaggta gggtgtcaag gtgaagttgg agcagcttgt tctatggcag cagcagcagt
     6481 agcatactta aaaggtggaa ctatagacca tattgaatat gcggcagaaa tagcacttga
     6541 acatcattta ggaatgactt gtgaccctgt atatggatat gttcaaattc catgtataga
     6601 aagaaatgca atggcagctc aaagagctta tgatgcagct aattatgcat tattaactga
     6661 tggctcacat tctgtctcct tagaccaagt tgttgaaaca atgaaagaaa ctggtataga
     6721 tatgatggat aagtacaaag aaacagctaa gggtggactt gctaaacatt tcttctcttg
     6781 ctaaaaataa aattttccaa taataattcc tctgttttga tttatgaaac aggggaatta
     6841 ttattaatgg tgaattattt aataatgcaa aatattttta tattcaattt agctttttat
     6901 ttttaattag tataacatgc tatataatat tttaattata aagaaatata gaaatatttt
     6961 ttaataaatt agaaattgga ggagattaca tgagagaggc atcatattta caagaacaac
     7021 tttataaaca taggaagtca ttaaatccta ttgcagagat tgggagaaaa gagtataaga
     7081 cttcaaaata tataagagat tatcttgata aattaggaat tgagtatgaa gtttatttag
     7141 aaacaggtat agtaggtgta atagaaggta aaagacctaa aaaagatatt gcttttagag
     7201 ctgacataga tggtcttcat agtgaagaag gggtaaaaca tttatgtggt catgatggac
     7261 atgctactat attacttgga ttaattgagt atttaaatga taataaagaa gacttaaatg
     7321 ataatatagt atttatattc caaccagcag aagaaggtcc tgggggagca aaagctttaa
     7381 tagatgaagg agtgcttaga aaatacaata ttgatgaaat atatgggctt catatatatc
     7441 cagagttacc agaaggttat gtaggtacaa gagagggcta ttttatggct caaataggag
     7501 atattgacat agaaatagtg tctaaaagtg gacatggggc tatgccacaa aatggtattg
     7561 atggtatagt gatagcttca aactttgtaa gctcacttca aactatagtg tctagaaata
     7621 taagcccaat agacaatgca gtgttgacta ttggcagaat tgaaggggga gctagaagaa
     7681 atataatagc tgaaaatata aagatggaag gtactatgag atgtttcagc ccagaagtgt
     7741 atgagaaaat gaagcttaga gttagagaac ttgctagagg gtttgaattg gcttataact
     7801 gtaaggttaa tataaatatt atagatgatt atatcgctgt taaaaatgac aaggatttgt
     7861 atcaagagtt tgtagaggct attggaagtg atactgttgt agagttagag ccacttatga
     7921 tatcagaaga tttttcttat tatcaaaaag aagttccagg attatttttt atgttgggtt
     7981 caagaaacga agaaaaagga tttgtaaatg gtcttcataa tataaacttt aattttgatg
     8041 aaaagatatg tgtaaatgca ttgaatgttt attcaaaatt attaaaatac aaagaagcaa
     8101 tagattagga agataagtta taaagattaa aaagcataaa tacctaaatt taaaaaaagt
     8161 gtattaataa atacccctta catatttgta taaatttatt ttaattgaca aaaatacaaa
     8221 taagaagggg tgattttttt gaaagaaaaa gtacaaagag aaaagaagat aactaatggc
     8281 aagaatacta aaatattgac aaatgatgat ataatgaaat atgagatagc ctcagaacta
     8341 ggtcttatgg ataaagttgg tgaacgtgga tgggcaggac ttacagctaa ggaagcagga
     8401 agaattggag gtatgcttac atcacgcaaa aagaggctta aaaagattgt ggaggagcag
     8461 aataaaaagg atgagtcaat atagtgattt tgcgtttgta tatgatgaac ttatgaatga
     8521 agttgattat aatggttggg ttaaatatat agaagatata atagatagtg aaggtgtaaa
     8581 agtaaaaaat atattggaat tggcatgtgg cacgggtaat cttacaattc cattgactaa
     8641 aaaaaattac gatatagctg gtatagatat atctgatgag atgcttagtg tagcaagaga
     8701 aaaggcagaa aaagaaggag tagaattagt tttacttcag caagatatat cagagcttga
     8761 ttttgaaata tctgatttgg actgtgtact ttgtgcttgt gatggattta attatataac
     8821 ttatgatgat gatttggaaa atatattttc aaagacatat gaattattaa agaaagatgg
     8881 aatattcata tttgatataa gttcttttta taaattagcc aacatattgg gaaataacat
     8941 gtatggagaa aatagagaag atatagcgta tatgtggcaa aattactttg atgaagaaga
     9001 gaatctaatt gaaatggaac ttacattctt tattaaagat gaatgtggaa agtttgaaag
     9061 atttgaagag atacaccaac aaagagcata tactgaagat gagatagttg atatgcttga
     9121 aaaatctgga tttaaggata taaaatatta tggggatttt acatttgaca aacctagtga
     9181 agaaagtcaa agaatcttct ttgtatgtaa aaaataagat attcttgact atataagata
     9241 attattaaaa ataaccatga tataaaaata tataaaatgc agttttatag attaaaagta
     9301 aagtgatgat attttatgac ttttattttc taataattat aaagttttat agataaaaat
     9361 taaaatataa taactattgc ttagtaaaat taaaataaaa tgggttttgc ccaaggttaa
     9421 gatagataag tatttataga taaattaagg tagtaatata ataaaaataa gacaattaac
     9481 gggacatgga ggaatattaa tgagagatta tgtactaaga gcgacatcag gaaatggtca
     9541 agttagagca tttgtcgcaa ctactagaaa tacagtagaa gaagctagaa gattacatga
     9601 aactacaaaa gtagcaacag cagcacttgg aagaacatta acagccacat ctataatggg
     9661 attaatgatg aaaaatgata gtgataaatt gactgtaata ataaaaggtg gaggtccaat
     9721 tggaactatt atcgccacat cagactctaa gggaatggta aaaggatatg taggaaatcc
     9781 acaagtagag gttgaagact atccaaatgg aaagttaaat gtagcagcag cagtaggtac
     9841 agaaggtgtt gtaaaagtta taaaagactt aggtcttaga gaaccatata atgggactta
     9901 tccattggta agtggagaaa ttgcagaaga ctttacttat tattttgctg tatcagagca
     9961 gactccatca gtagtagctt taggtgttct tacaaaagaa gatgaagtag agtttgctgg
    10021 aggatttata gttcaattaa tgccagatgc agaagaagat actattgcta agttagagga
    10081 aaatgtagct aaattacctt ctataactaa catgttaaaa gaaggaaaat caccagagga
    10141 tatattaaat attgttttgg atggtttgga acctaaaata cttgatacct gtgaagttgg
    10201 gtttatgtgt gaatgttcaa aagaaagagt aaaaactgct ttggttgcaa ttggtaaaaa
    10261 atcattggct caaataatag aagaagataa aaaagcagaa gttggttgcc agttctgtaa
    10321 taagaagtat atgtatagtg aagaagagtt gcttgaaatt ttaaaggaaa tgtagactat
    10381 aaaaatagtt gtattgatat aagtaatgtt atagtgatat agtaaagtcg tattgatata
    10441 gtaattaaag tataatttat taataataga catatttata ttaaaaaata agtttaataa
    10501 atttttaagc ttaataaatt ttaagaatta agaagctttc tggctgtaat gtttgcaatg
    10561 ttgcagttag aaggcttttt tattttatta tagttcgtgt caaagattgt atgaaacaat
    10621 aaaatattgt aaaataattt gtatatttag acctgattga cacaaaggtt tataagatat
    10681 ttgagttgat tatacgaaat tctatgaagt agtaaccctg tgactttaat catgggaggt
    10741 tcagaagaag gagaaaattt taaatatgga aagtaaaacg aagtacatag gaataagtat
    10801 attaatatta ttgatgatat ttttaattgc aaagaatttt aacggaaaac ttgaaatacc
    10861 tattcctgag agtaatagta gaaaatttaa atcagcagca gtgttttatc ctcaacatca
    10921 agatgatgag gttttatggg gaggaagtgc cattgtagat gctataaaac agtgtggtgt
    10981 tgataatgta tatgtggtat tagtatcaga tggttctgga gttaacgttt ttaaagctaa
    11041 cacaaaattt agaaatttaa ctaggaagca aaaggaagaa cttagaaata acgagtttaa
    11101 atcagctttg agagagcttg gagtgaaacc tcaaaatgtg attatacttg cagatataga
    11161 taaaaaggaa ggcacacatt atgaattaat ggaaaagact atattggatt ttgaacataa
    11221 gtttaaaggc aatgtgaccc atatagccca tcactataag tatgatgacc atataatgca
    11281 tagaaagaat ggggaagttt taaagaaact taaacgtgaa ggtaagataa aagatgactt
    11341 gtattttgtt aagccaaaat atgtaaagta tataccaaga aatgaaagag ctatctataa
    11401 agcaatggat tttgatgact acaataaggt taagagtgct tgttatgaat ataaggttgt
    11461 aaattcaaag cgtaaaatgt atggtatagg atatatttcg gcacatagtt attttgaaca
    11521 tcttctaaat gaccctgaac ttacgtctgt actaagtgaa gattaatttc ttataagtag
    11581 taagttatgt ttgataagtt gattggaatt ttctatagtt ttgaaggtat tggaaattta
    11641 gttttattta aagatttaaa gatagaggtt tttaatgttt aaaattttaa agttaagttg
    11701 aataattgta tagaattatt tgtgaaagaa gaaacagtgt aaaacatagt atcttattta
    11761 tggttttaaa atcataggca agatactttt tttatgtaac taataccatt ttgagattat
    11821 taacttaaga attttatata atttaacact catatataat gatgatattt aggtagaata
    11881 gatgtaatta cgcttaaata aaataactgt attttttata aaaatcaaac cctacactat
    11941 actaccagat aaaatctaca tgctataatt tgacctgtgt ttataaaaaa aggagtaatc
    12001 taataaaata atatttcact tatgtataac taacaactat tattacgatt ttctctaaat
    12061 aatttaaaga aaaataatgc aggaggtaaa atatgaacaa tgaatttata attttaaaag
    12121 gttgcaaaga gaataatttg aacaatattt ccttaaaaat accaaaaaga aaaataacca
    12181 tattcacagg ggtgtcgggt tctggaaagt catctattgt atttgagaca attgcaaaag
    12241 aatcacaacg ccaactaaat gaaaggttta gtacatttgt aagaagtttt ttacctaagt
    12301 atggagaggt taaagctgat tgtatagaaa atttgtcaac accaattatc atagaccaaa
    12361 gtagacttgg tggaaattca aggtcaactc taggtactat tacagatgta aattcctttt
    12421 taagagcttt atattcaagg tttggtagtg agtatatagg taatgccaat atgttttctt
    12481 ttaataatat tgatggtatg tgcccacact gtcatgggct tggaaaaaag ctagttccta
    12541 atatgaacca aattcttgat atgaataaat cattaaatga aggtgctata ttgctttcag
    12601 ggtttggagt gggttcttgg cattggaata tattcgctga atcaggattt tttgatatta
    12661 ataagaggat atgtgattat tcagaagtag aattggaaaa gtttttgcat ggtaaagcag
    12721 aaaagattaa gatagaaaat gctggtcaaa ctaacatgac atatgaggga ttaatggtta
    12781 aatttaatcg cctatactta agtagagagg gagaaatatc tgaagctact aagaaaaaat
    12841 tgagtaaact tcttatagaa gatagatgtc ctatatgtga tggaagaaga cttaatgaaa
    12901 gagtatatag gtctttaatt aatggttata atatagcaga tttaactagt atgcaaattg
    12961 atgaattggc tgaagttatt aaaagtatag atgagccaga agcacaacct ttaattaaag
    13021 gaataataga aaagttaaac agtatcatag atataggtct tggatattta acactagata
    13081 gagagacatc ttctctatca ggaggggaat ctcagcgtat aaagatggtt aagtatttaa
    13141 atagtaatct tgttgattta atgtatattt ttgatgaacc aagtgtagga cttcatccaa
    13201 aagatgtgta taaattaaat aatcttttaa aaaaattgcg tgataaaggg aatacaatta
    13261 tagttgttga acatgaccca gatgttatta agattgcaga ccatattgta gatgtaggac
    13321 caaaggcagg taagtatggt ggagaagttg tgtatgaagg cagttatgaa aatctactta
    13381 caagtggtac tttgacaggg aatgctttaa gtaaatttct tcctattaaa gaaagtgtaa
    13441 gagaacatag tggatattta gaagttaaaa attgtaataa aaacaattta aaaaatgtat
    13501 ctattaagat acctaaaggt gtattgactt tagttactgg agtagcagga gcaggtaaaa
    13561 gtacgttgat taaggatgaa tttttaaagc aaaatcctag tgctgtactt atagaccaaa
    13621 gccctgtttc agcaaattca aggtcgagtt tggcaacata tagtggaatt atgaacaaca
    13681 taagaagcat attttcaaaa acaaatggcg taaatgcttc tttattcagt tcaaattcag
    13741 aaggagcttg tgataactgt aagggttctg gaatagtaga aatgaatctt gcttttatgg
    13801 agagtataaa gagtacctgc aatgtttgtg aaggtaaaaa atttaaaaaa gaagttttag
    13861 aatataggtt tcaagggaag aatataattg aagttcttga aatgtctgtt ttagacgcaa
    13921 ttgagttttt taatttaaaa cagattaaaa cgaagttaca atgtattgaa aaaatgggta
    13981 ttggatacct tactttagga caaactcttg atactttgtc tggaggagag tgtcaaagat
    14041 taaagcttgc tagtgaatta aataaggaaa gttcagtgta tatattagat gaaccaacaa
    14101 caggtcttca tatggctgat attgagaatt ttataagtat aattgaagag attgtagata
    14161 atggaaatac ggtcataata attgaacaca atatagatgt aattaaaaga gctgattgga
    14221 ttattgagct gggacctgaa ggaggaacaa aaggcgggag agtaatattt gaaggtattc
    14281 caaaacaatt atgtaacaat aaaatatcat taacagcaaa atatatagta taataaataa
    14341 aatataataa aattataatg atattttaat aaatataatg ggtatataaa atgataatta
    14401 aacttaacag taataaataa tgtgagaaat attcaaattt aattaaataa agtgtcagaa
    14461 atttgtttga attttagtat tttaacggtt ctgacctttt ttcttatttt tatggagaat
    14521 atcgaggatg gagtctctaa tagttacttt tttgtgtcta cttgcaagtt tgattaaaaa
    14581 atggctatac acacttgtga cttcagtcat gggaggttca gattataatc ttaatatata
    14641 atttgtgaaa taatttgatg acgatttgct gatgtataaa caataaagat taactacatt
    14701 aaaatttaaa tcttgttaag taaaacttat aaaaggagag agatggatga taaatgatat
    14761 tctaacctta ttatctgaaa gaagtgattt ttttataaag ctatccatag aacatttgat
    14821 gatttctatt atttctataa taattgcgat tgttttgggt ggtataattg gtattattgt
    14881 aagtgaattt cataattctt ctaaacctat attgggaact atcaattttc tatacaccat
    14941 tccatctatt tctatgctag gatttctgat accattttct ggaattggaa atactactgc
    15001 tgttattgca cttaccattt atgcattatt gccaatggtt agaaatacat atactggaat
    15061 tactaatgtg gataaaaata ttcttgaagc agctagggga atgggaagta cagattttca
    15121 gatattgtat aaaataaaac ttccgttagc cctacctgtt attatgtctg gtattagaaa
    15181 tatggtgatt atgacaattg cattagctgg tatcgcttca tttataggtg ctggaggcct
    15241 aggtgtagcc atatatagag gaatcacgac aaataacaca ataatgacaa ttactggaag
    15301 tatacttatt gctctattag ctttaatatt tgattttatt ttagggctgg tagaaaaatt
    15361 ttttcaacga aaaagaaaaa agaaaaatag gaaaaaagtt tttgccattc taggaagcct
    15421 tattcttata gtaattatct ttacgtctta tttatttaat ccttcaaaga ctcaaactat
    15481 tcatattgct actaaaccta tgactgaaca atatattctt ggtgagatgt tggatatttt
    15541 aattgaaaaa aacacaggtc taaacgtgga aattacacaa ggtgtaggag ggggaacatc
    15601 taatatccag ccagctatgg agaatggaga atttgacatt tatccagagt atacaggtac
    15661 aggctggaat atggtactta agaaaaatga attatatact gaaagtatgt ttagtaaact
    15721 acagtcggaa tacaaaacta aacttaaaat gcaatggact ggtatgtatg gtttttctaa
    15781 tagctatgca ttagcagtta gaaaagaagt tgcagataaa tacaatttaa aaaatataac
    15841 ggacttaaaa gatgtatctt ctaaattgac atttggagct gaatatgact tttatgagag
    15901 agaggatgga tatactccat tgtgtaatac atatggactg aactttaaag atacagttga
    15961 tttagatatt ggattaaaat accaagctat caatgaaggt aagattgacg taatgcctat
    16021 atttacaact gatggtcagt atagtgtttc agatgttgta gttctaaaag atgataaaaa
    16081 tttctaccca tcttatcaat gtggaaatgt tgttcgtttg gaagtattag aggaaaatcc
    16141 agaactatca aaggtattta agatggttgg agatattttg actgataaag aaatggctaa
    16201 aatgaattat gacgtagaaa acaacaattt agaacctaga gaagttgctc gtgagtttct
    16261 gaataagaaa ggtcttttat aaattggagg aaatataatg acacctatta tacaatttaa
    16321 aaatattaaa aaacaatata atgataaaac tataatagat aatcttaatt tagatataga
    16381 aaaaggagag tttttgactg taattggttc gtctggttct ggtaagacaa cactcttaaa
    16441 aatgattaat ggtctgattt tgccagatgg aggaaatatt ttgataaacc aaacagatat
    16501 caaaaacgag gacttaatta aattaagaag aagaatagga tattgtgtac agggaagtgt
    16561 tctttttcct catatgactg ttgaagaaaa tatttcatat gtaccaaatc tcctaagtaa
    16621 aaagaataaa ttagaagtaa aaagtgctgt aaacaagtgg atggaaatag tgggattgcc
    16681 aaatgatatg aaagtgcgtt acccatcaga attgtcaggt ggtcagcaac aacgtgtagg
    16741 gattgcaaga gctttagctt cttctccaga aattttatta atggatgaac cttttggtgc
    16801 agttgatgag attacacgaa agcaattaca aaaagagata aaagaaattc ataaaaaaac
    16861 aggagttacg attatcttta ttacacatga tatttatgaa gctttgattt tgggaacaaa
    16921 gactttggta ttaaatcatg gagttgttca acagtatgat actcctgaga atattttaaa
    16981 tactcctgct aaccaatttg tagaccagtt attgaatata agaaaatcta taatagatga
    17041 tgataattta aaagaataaa tttaaattta aaatcaaatt gttgtgataa aaactattga
    17101 tttagagcta acttcaagtt gtatacttga agtggaaaag ttagattaag gtatagaagg
    17161 gcatttagca gttggctgta gacgaggtaa ctgctgaagg tgctttcaat aaatggctag
    17221 aacctgtatc tgttgaagat tataataaat tgaaataaga agctcaatat acagagtaga
    17281 taggcacttc aaatagaagt gtcttttgtg tgccataaaa taaatcaaaa tattagcaaa
    17341 gaatgtgaaa aacaaaataa aataatatgc tatctttaaa ataggagggg tgctatatgg
    17401 ataacaattt gattaccatt gaaaaaactt tagattatat agaaaaacat cttttagatg
    17461 agatgttaga tttaaatgta atttctaaag agataggcta ttcaaaatac catttacata
    17521 gaatgtttac atcagttgtc ggatttagtg tacataatta tatacaaaga cgtagactta
    17581 ctgaagcagc gagaatgcta atttttacag aaaaaccaat tattgagatt gatttaatct
    17641 cggggtatga tacacaacgc tctttttcaa gagggtttaa aaaaatgttt aaacgtagtc
    17701 ctaatttttt tagaagacat caagattttt taccactaca attaaaattt gatattcata
    17761 atagaaaaaa attacgtgga gatatggttt tagatgttaa aaaagttaat tgtgcagata
    17821 taaatattgt tggctattct ggtaaagtaa gaaaaggatt tcatattatt ggaaaatgct
    17881 ggagggattt gcataaaaat aaagagatga tagaaaataa ggtagattta ggttttttaa
    17941 ttggagttag tgattatagc aattttaaag agattgagaa tactcctaca tttacataca
    18001 ttgcaggtgc tcaagtagct acttttggca aattacctaa tggaatggaa aaattcgtat
    18061 taccgaaaag taattatatt gtttttactt ttattgggaa aaatgaggat agtatgcaaa
    18121 atgttgtaga atatatctat caagaatggt tccctaattc aagttgccag tttaatgaaa
    18181 ataatttata tgactttgta aaatatggag aaaaaactga tgagaatggt ttgagtgaaa
    18241 ttcagttttg ggttccaatc atttaaaaga aggtattatg tataaagtag atggatttat
    18301 ataggcaaag ttttacagca gtatgtattt ataaataatt tata
//
LOCUS       DAEMHZ010000075        17917 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000075 DAEMHZ010000000
VERSION     DAEMHZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.075"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            347..1180
                     /locus_tag="KPK72_002362"
     CDS             347..1180
                     /locus_tag="KPK72_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2489570.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1273..2205
                     /locus_tag="KPK72_002363"
     CDS             1273..2205
                     /locus_tag="KPK72_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2489571.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2257..2739
                     /locus_tag="KPK72_002364"
     CDS             2257..2739
                     /locus_tag="KPK72_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2489572.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2751..3620
                     /locus_tag="KPK72_002365"
     CDS             2751..3620
                     /locus_tag="KPK72_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2489573.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3663..4418
                     /locus_tag="KPK72_002366"
     CDS             3663..4418
                     /locus_tag="KPK72_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2489574.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRTISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4460..5281
                     /locus_tag="KPK72_002367"
     CDS             4460..5281
                     /locus_tag="KPK72_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG2489575.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5286..6047
                     /locus_tag="KPK72_002368"
     CDS             5286..6047
                     /locus_tag="KPK72_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG2489576.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKFLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6216..6473
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6586..6825
                     /locus_tag="KPK72_002369"
     CDS             6586..6825
                     /locus_tag="KPK72_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2489577.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7129..8124
                     /gene="ilvC"
                     /locus_tag="KPK72_002370"
     CDS             7129..8124
                     /gene="ilvC"
                     /locus_tag="KPK72_002370"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG2489578.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8177..9865
                     /gene="ilvB"
                     /locus_tag="KPK72_002371"
     CDS             8177..9865
                     /gene="ilvB"
                     /locus_tag="KPK72_002371"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG2489579.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10076..10753
                     /locus_tag="KPK72_002372"
     CDS             10076..10753
                     /locus_tag="KPK72_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2489580.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10927..12963
                     /locus_tag="KPK72_002373"
     CDS             10927..12963
                     /locus_tag="KPK72_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG2489581.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDENYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13154..13720)
                     /locus_tag="KPK72_002374"
     CDS             complement(13154..13720)
                     /locus_tag="KPK72_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2489582.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            14008..14943
                     /locus_tag="KPK72_002375"
     CDS             14008..14943
                     /locus_tag="KPK72_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2489583.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15408..16622
                     /locus_tag="KPK72_002376"
     CDS             15408..16622
                     /locus_tag="KPK72_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489584.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(17042..17563)
                     /locus_tag="KPK72_002377"
     CDS             complement(17042..17563)
                     /locus_tag="KPK72_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2489585.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
BASE COUNT         7359 a         1766 c         3036 g         5756 t
ORIGIN      
        1 tatgaatata taatgtatgt attaaaaatg tggcaaatag ttgttttgct atttattatt
       61 tgtttaatat ttctaattta taaaaaaaaa ttataaattt taaaaattat aaatgacatt
      121 ttaactagaa ttaattgtaa aaaacactat ataatactca attttcaaca ttctatacaa
      181 ttatacagaa attatacagc ataataatca ccgaaataac aaattttaaa aattaagcaa
      241 aactttttgt cgaagttcaa cgtctatata tatgtagtgt aaatttttgg gataaaatac
      301 tacaaaaata taaatgtaaa aatatagtaa tattggaggt cgaacgatga gaaataaaaa
      361 gaaaaaaata aatagaaaga agttgtattt gcttttaagt gctattgtag tgtgtctggc
      421 ttttatagta gttggtgttc gcgtcagctc tttaaatatg aaggaaaatg aaattagtag
      481 aaatgcaaaa ctgtcaagtt ctactattac agatatagta tctaatacat caatagaagt
      541 tagtaaaggg ccaagtaaaa gttctggaaa aatagcatac ataactatag atgatggtcc
      601 atctaaattt actgaccaga tgataaaaac tttaaataaa tacaatgtta aagcaacttt
      661 ttttatgata gatgggaata tgaaggaata tccacagcaa gttaaaaata taattaaaaa
      721 tgggaacaca gcaggttttc atagtgtatc acatgatatt cacaagcttt atgttactag
      781 tacatcagca aaagaggaat ttgatacaaa tgatcaaact ttttataaga taactggtaa
      841 acattcaaaa gtaataagaa taccttatgg aagcaaacca tatacaccac aagcctctta
      901 tcaggcttta gttgatgctg gatataaaat ttgggactgg gacttagata cagaagactg
      961 gagatctaat tcttctcaaa ttgtacaaaa tgtaaaaaat catattaaga atagaaaagg
     1021 tgaagataaa gaccaacttg tagttttaat gcatgagaaa aaacagagtg cagaagcttt
     1081 agattcagtt ttaaaattct tatcagatga aggttatgaa tttgcgccag tagatcaaaa
     1141 ccaaatacca aaaaattatt ggttacgtaa tttagagtag ttaaaatata agaatattga
     1201 ttgatatttt tagaaattga gaataaaaat gagtgaggtg taggtatatt gaataaaaaa
     1261 atttatatag gaatggtcgg aatattgtct ttgatgatgg ttggttgtaa taaaagttta
     1321 gcaaaagtaa atgatgtgga aatcacaaaa gaacagtaca aaaaaacaaa agcagtttta
     1381 tctgctacta ataattatat aaatgggcag tctttagatg aattagaaaa aactttggat
     1441 aaaaaaggta gaaataaatt agaaaatgtt ataatatcgt ttatggtgga taatgaactt
     1501 ttatatcaag aggctaagga taaaggacta actccaagta aaagtgaagt agactcaaaa
     1561 tatcaagaac tagaagataa gatgaatttg aatacaagct ataaggaaaa aatggacaaa
     1621 gcaggtgttg ataaagatta tttaaaacaa gaaatatcta gagatttagc aatagataaa
     1681 aataaaaagg cctttgaaga tagaataaac ataagtgata atgatatgga agcctactat
     1741 acaagtcata aaaaagattt taatgtagaa gaagtaagtg cttctcagat acttatatcg
     1801 actttagata aaaataaaaa agaagtaagc aaagataaaa aagaagcttt gaagaagaag
     1861 gcagataata tattgactaa aatcaaaaat ggagaaagtt ttgaaagttt ggctaaaaaa
     1921 tattcggatg ataaagcaac tggaaaaaat ggaggacagc taggatattt tacaaaggat
     1981 gataaaaatg ctgagtttac aaaggaagtg ttcaaattaa agaaaaatga agtttcaaat
     2041 gtgtttgaaa ctagttatgg atatcatata gtcaaagtta ctgataaaag agaaagacaa
     2101 aaaagtttta atgagtgtca gagtttaata agagaatcta ttttaaatga aaagtatatt
     2161 gagcatataa aaaaactgaa tgaagatgct aaaatagaca gatagaattt ctaacagctc
     2221 aatcctagcc tgtaatcgga gggagatttt acaggaatgt ttaagagttt aaagagaacg
     2281 aaagttgaga aatacattat cgataataaa gactcttttt atagaatagc atatagttat
     2341 acgaagaatg aagaagatgc cttagatgtg gttcaagaag ccatgtataa ggcactatac
     2401 tcagtggaaa atattaaaga agtaaattat ataaagacat ggttttataa aattttagtt
     2461 agaacgagta tagattttat tagaaaaaac cgtaaatata ataatatgac agatattgac
     2521 cttatagatg agactggaga gtatgataaa tatactgatt tggatttaag aagagctttg
     2581 gaagaattac ctattgaata caaatctatt ataatattaa gattttttga agatttaaag
     2641 attgaagaag tagcaataat attggatgaa aatgtgaata ctgttaaaac taggttatat
     2701 actgctttaa aaaaactaaa attaaaaatt gaagagtagg gataaaaaga atgacaagca
     2761 aagaaaggtt aaataagtta aaagatgagt atcataaaat tccaataccc aagaaattag
     2821 atactataat aaatcatgaa aaaatagaaa acatatatcg agagaaaaat aaaaaagtaa
     2881 atagattaag atttaaagtt gcaatagcct ttgcatgtat atttacagta ttagtaaata
     2941 taagtcctgt atttgcagac aatttttcta agatacctgt aattggagca atagtagaag
     3001 taataactat aaaaaattat agtttaaaaa gtgaaaatta tgaagctgag attgatattc
     3061 ctaaaatacg aggtttaaaa gataaaaact tagaacaaag attaaacagt agttttatgg
     3121 aagatggaaa aagactttat catcaattcc aagagagaat ggagaagata caatcatcaa
     3181 aaaataaagg atataaatct ttaagtttga gttatagtgt aaaaaataat tctaaaaaat
     3241 ttctatcaat agagatgaca aaaaatgaga tagaggcttc atcatatgtg agtaaggtac
     3301 attatactat tgataaaaaa agacaaatag ttttgacttt gccaatgctt tttaaagatg
     3361 ataagtatat aaaagttata agtgataata taaaagaaca aatgagagaa caaatgaaaa
     3421 aggatagtac aaaatcctat tttatagatc aaaaaaaaga cttgcctgtt gaagatttta
     3481 aaacaattaa taagtatcag gatttttatt ttaataaaaa tgaagacctt gtaatttgtt
     3541 ttgatgaata tgaagtagca cctggttata tgggcgcagt tgagtttgta ataccatata
     3601 aagtaataaa agatttgtaa attgaatagt agtttatata taaatctttt aaggaggaca
     3661 atatgactac atataaaaaa cttaaaaaat ctatatttaa aattgctata ttttcaatat
     3721 gcttattagt ttcattagta gatacaaacc ctatatttgc aggtagttcc tttaatgaaa
     3781 cttcaaatag tgatacgata aaggtgttaa atgctaagag tatcaacttt aaacaagaca
     3841 attatgaagt gaatgtgagt attcctaaaa taaaaggttt aaaagataaa actttagaga
     3901 ataaattaaa caatgagctt ttgcaagatg ggaaaaaaat ttacagtgaa tttcaaaata
     3961 aaatgaaaaa ggaagataat aagggtcata gatacctaaa tgtaatccat aatgtcaaaa
     4021 ataattctaa ggacttttta tcaatagaaa tgacaaaaga agagatagaa gcttcctcat
     4081 ctataactaa gaagcattac actgttgata aaaatagagg aatactatta aatttaccaa
     4141 tattttttaa agatagtaga tatattagaa ctataagtga taatataaaa gagcagatga
     4201 gaaatcaaat taagaaagat gatacaaaat cttattttat agaccaagat aaagactcgc
     4261 cagtgaaaga ttttgaatct ataaataagt atcaagattt ttattttaat aaaaatggaa
     4321 atcttgtgat tagttttgat gaatatgaag tagctcctgg ttatatggga gcagtagaat
     4381 ttgtgatacc aaataaagta ataaaaagac taagatagat atatagaaga tttttagata
     4441 gactggtaag gagaattata tgaatatata tgaacaagca ataagattaa tagaaaataa
     4501 tgaggatttc gcatttgcta caataacgtc tcattcaggt tcaacaccac gtgaaactgg
     4561 agcaatgatg attgtgaaaa atgattctac tatatttggc tcagtaggag gaggtagtgt
     4621 agaagcagaa tgtataaaac atgcaattaa tgttataaaa aacagagaat caatgttgta
     4681 taaatttact ctaaacaaat ctgatgtggc aaaactagga atgatatgtg gaggtactgg
     4741 agaaatacaa atagatttta tagatagtaa attaaaaagt aatatagaaa aatttaataa
     4801 gagactaaag gaaaatacaa gtaaagcata tatatttggt gcaggtcata tttcaagaga
     4861 tgttgctgtg atactatctc ttttagaatt tagaacagta gtaatagatg atagagaaga
     4921 atttgctaat catgaaaggt ttccagaatc agaagtagta gttttagatt cttttgaaaa
     4981 cataccagat tttccaactg acgaaaatag ttatataatt attttaacaa gaggtcatct
     5041 atatgattca agtgctttag aatgggctct taaaagagag gctggttata taggtatgat
     5101 tggaagtaga acaaaaattg gattgactta cgaaaaactc atgaaaaaag gttttaagaa
     5161 ggaagaatta tcaaaagtac atgcaccaat aggcataaaa ttgaatgctc aaacaccagc
     5221 tgaaatagct gtatgtatag ctgctgaact tataaattgt agagctaata aggaaaaata
     5281 aactaatgaa cacagggagt aaagatattg atactttaaa ggttaatttt agagggattg
     5341 aaataaaagc agttttagta tataaaaata gaaaaaatat ttctataaaa atagatccat
     5401 ttggcaaaat aactgtaatg agtcctccta aaatatccaa aaaaataata aaagatataa
     5461 ttattgaaaa aggagactgg atacttaaaa agtcagaaaa atataaagga agagaggaag
     5521 tatataaaca aagaatgttt ataactggcg aaaaatttct ttatttaggt gaagagtacc
     5581 atctagttat aaaagaactg ttgagagatt ctgtcaaaaa aagtaactgt agaataacta
     5641 ttaacgaata tcaaatagtt gttcagacaa atgatacaag tacagatttt attaaaaaat
     5701 ttttaaaatc ttggtataag atggaaagtg aaagaatagt attagagaga atagattttc
     5761 ttaaattaaa ttgtgaaata atgaagcaat tagttccagc aagtgttaag gtaaaagaac
     5821 aaaataagag atggggaagt tgtacagctc aaaaaaatat ttatataaac tcaaaaatat
     5881 ctatggcaag attagatgta attgattatg taataataca tgagtttagc catttagtac
     5941 atatgaatca ctctaagaaa ttctatgatt tagtaaaatc cattatgccc gattataaag
     6001 ataaagaaaa ttggctaaaa ataaatggat ataaaataat aatctgaaaa cgattattcc
     6061 aaaatatagg cgtaaagtat aaaagaagta ttattgtgaa aaaaataaat aatctgaaaa
     6121 ttttaaaaaa agtcattgac attgtaaatg atagggaata taatctatat taacaacaaa
     6181 acagaattaa aatataaaat taaaaaatta aaaaattaaa agcaatgaca gagacaacaa
     6241 tactaatagt gttatccaca gagaaccaat gttgctgaga attggtgtta acagtagtat
     6301 aatgatcact ctggagcttt tgtactgaat atttttagta agtacaaacg tatgtcaacg
     6361 ttacatgact aaggttaagg taattaatta cccgacctga aaaaagaggg gtagatgtat
     6421 tttttatatc tacaaataag gtggtaccgc ggaatataac tttcgtcctt ataagtaata
     6481 tttatattgc ttataaagat gaaagttttt ttattttaag cgatataaat aaatttaatc
     6541 catataaaat taataaaaaa ataataaaaa taaggaggga tttttatgga aaatactgta
     6601 tatgctaaat taaaccaagg aatagattct tttttaagag tggctatgac tctaagaaga
     6661 agagaggttg atatacaatc aataagtatg actgtagata atatgaataa ttcaggtata
     6721 aaattaatag taaatgaaga aaagacatct ttagattctg tcttaaacca tatgaaaaaa
     6781 ctacatgata taagagagat aactgttgaa agaatgagac attaattaaa tatattttat
     6841 aaagctaaag ataaatatgc ttattttaaa ataaaaaact aaagataaat attaaataag
     6901 aaaaatctta gattaaaata taagtgtcat tgaatttatt aatttatcac agttaataaa
     6961 tttattagtt tgttgtagct aataaaaact gaaaattcta aaattataat tgtaagatta
     7021 tataaattta gaatttaaat ctgttgaata atattaaaac aatgataatg agtatgaatt
     7081 tctaataaaa ataaatatca taaaaataag aaaaagtatt gggaggaaat gaaaatggca
     7141 agaatgtatt atgaaaaaga tgtggattta gaggttttaa aaaataaaaa ggttgcagtt
     7201 ttaggttatg gaagtcaagg acatgcacat gcacaaaatc ttagagataa cggtgtacat
     7261 gtaatgatag gtctttatga cggaagtaaa tcagcacaaa aagcaaaaga agatggattt
     7321 gaagttaaga gtgtagcaga agctacaaaa gaaagtgatt tgacaatgat gttaatgcct
     7381 gatgaaaaac aaaagaaagt ttatgaagag agtgtaaagg ataacttaaa agaaggtcag
     7441 acattagctt ttgctcatgg attcaatata cattataatc aagtacaacc accagaattt
     7501 gttgatgttg taatggtagc tcctaaagga cctggtcact tagttagaaa tgtatttaca
     7561 aagggaagtg gagttcctgc actatttgca gtatatcaag actacactaa aaaagctaca
     7621 gagacagttt tagcatatgc aaaagggata ggagcaacaa gagcaggtgt tttagaaact
     7681 acttttaaag aagaaacaga aacagattta tttggagaac aatcagtact ttgtggtgga
     7741 ataagtgaac ttataaaatt gggatataaa acgcttgtag atgcaggtta tcaaaaagaa
     7801 gtagcatatt ttgaatgttt acatgaaatg aaactaatag tcgaccttat atatgaaggt
     7861 ggatttgaaa gaatgagata tagtataagt gacacagcag aatatggaga ttatgtatct
     7921 ggtaaaaggg taataactga tgctgcaaaa caaggtatgc aaaatgtttt agaggatata
     7981 caaaatggaa aatttgcaaa agcgtggatt aaagaaaatg aagaaggacg tgaaaacttc
     8041 ctaaaaacta gagaagaaga atataataca gaaattgccg aagttggtag aaatctaaga
     8101 agtatgatgt cattcttaaa ataaatatat attaaatgga ttagtttgag ggaaagagtg
     8161 gtgtatagta gtgcgtatga atggagcaaa ggtaatttta gaatgtttaa agaaagaggg
     8221 aatagacaca atatttggat atcctggtgg agccgtgata cctctttatg atgctctata
     8281 cgattattca gatgatttta aacatataag gacatcccat gaacaaggtt tagttcatgc
     8341 tgcagatggg tacgcaagaa gtactaatac agtaggagtt tgttttacaa catctggtcc
     8401 aggagcaaca aatgccataa ctggaatagc aacagctttt atggattctt cacctatggt
     8461 tgtaatttca gggcaggtgc caactagttt attaggaaaa gattcttttc aagaaattga
     8521 cataactgga gcaacattat ctatgacaaa acataattat ttagttagaa atactaaaga
     8581 attagttcct acaataaaag aagcttttag agttgcaaat tcaggtagaa aaggacctgt
     8641 acttgtagat gttccaaaag atttattttt agcagaaatg gattttagtg gtgaggacta
     8701 tgatttatgt caaatagatg attatatgga ttataagagt gattttgatc tagatgatga
     8761 aactaatatt aagcttttaa atgaagcaat agatatcata aaagaatcta aaaagcctgt
     8821 aatatatgca ggtggaggtg ttaaatcttc agacagtgag gaaattcttg aaaaatttgc
     8881 aactaagata gatacacccg tactaaatac acttatgggg cttggaaata tagatagaaa
     8941 gaatgaacta tctcttggaa tggttggaat gcatggaagt agagaaagta atttagcact
     9001 ttctaattca gatttagtga tagcgatagg tgcgagattt agtgatagag tgataagtaa
     9061 aagttctgaa tttgctaaaa atgcaaaaat aatacacata gacatagacc catctgaaat
     9121 aagtaaaaat atagaatcta atgtatcttt ggttggagat gtaaagctag tccttagttt
     9181 attaattgaa agggttgaaa gtaagaataa tagcaattgg aaagaagaga taaagacatt
     9241 tagaaagagt gagggtgtac aaacatatga atttcatcca caaaatatac ttaaaaaaat
     9301 aaatgaaaaa tatgaaacat taaagaaacc cactgtggta gtaactgatg tcggtcaaca
     9361 tcaaatgtgg tctgctaaat actggaattt taaaggtaat aaaagtttta taacatcagc
     9421 agggttagga actatgggat ttggtttagg tgcagctatt ggaactaaag ttggtaatgt
     9481 agataaaaat gtagttttag tcactggtga tggaagtttt agaatgaact gtaatgaact
     9541 agctacagtt gcaaattata atgtacctat gcttatttta ctactcaata atagaacatt
     9601 aggaatggta aggcaatggc aaaagttatt ttccaatcaa agatactctc aaactgacat
     9661 taatgaaaat gtggattatg ttaaactcgt aaatgcatat aatattgatg gatataaggt
     9721 atccagtatg gaagaattag gaaaggcgct agatatgata gattttaata aacctgtatt
     9781 tttgcaatgt gatatagata aggattatga tgtttatcct attgtagcac ctaatgatgc
     9841 attggaaaat ttgatatgta actaaaactt atactgaata ttgataaaat tttttattaa
     9901 atttttattg gtattcagtt tttgttgttt agagatagat tggcattttt tattttttca
     9961 aataaatcaa tgactatata attaagaact tattaaattt taattataca atttggaata
    10021 gtattacaac ttaagtaaat aataattttg aataataatt tacggaggta ttcatatgtt
    10081 taaacacgat aaagcattat taaaagaagt aaaagtagaa agaccaaatc ctcaatatgc
    10141 tgttttaatg caagaacagt tgggtggtgc aaatggagag ttaaaagcag ctatgcaata
    10201 cttgtcccaa agttttagaa taaaagaccc tcaaataaaa gatttatttc ttgatatagc
    10261 agcagaagag cttagccata tggaaatggt agcacaaact ataaatttat taaatggaca
    10321 tgatgttgat tataattcag ttaatacagg cgaaatagaa acacatgtat taactggtct
    10381 atcaccagtc ttaataaact catcaggagc tccttggact gctaattacg ttacagttac
    10441 aggggattta gtagctgact tactttcaaa tatagcttct gaacaaagag ctaaggtagt
    10501 ttatgagtac ttgtatagac aaatagatga taaatatgta aaagaaacaa tagatttctt
    10561 acttaataga gaagaagcac ataatgctct atttagagat gctttaaata aagttaaaga
    10621 tacaggttca aatagagatt ttggagttac agaagattct aaattatatt tcgatttgtc
    10681 tactccaggg ccaaatcacg atactaagat agatattaat cctccttctt ttgaaaaacc
    10741 tttaaaaaaa taatttgata ttttttatgt tgttttgttt tagtagttat aaaaattatt
    10801 tattagaaga attaattata tgaaaaatta aatatgtata gttaaaaatg ggtatacact
    10861 tattataagt gtatatctat ttgtttattg caaaacatat aataaaataa caaatgaggt
    10921 ggtattttgg acagtaatag aaaacctaat aatctaataa atgaaaaatc tccatatctt
    10981 ttacagcatg cttataatcc tataaactgg tatagttgga atgatgaggc ttttaaaaaa
    11041 gcaaaagaag aggataaacc aatattttta agtgtaggat attcaacatg tcattggtgc
    11101 catgttatgg aaaaagaatc ttttgaagat gaagaagttg ctgaaataat gaatagaaac
    11161 tttgtggcaa taaaagtaga taaagaggaa agacctgatg tagatagcgt gtatatgaca
    11221 gtttgccaag ctatgacagg tagtggtgga tggccaatga ccataataat gacaccagat
    11281 aaaaaacctt tttttgcggg aacatatttc ccaaaatatt caaggtataa tagacctgga
    11341 gttatagatt tattagagaa tgtatctgaa aaatggaata caagcaggga tatattaata
    11401 aaatctggag atgaaataat agaagctctt aaggatgatt ttggtgtaaa aaatactgag
    11461 ggtgatttat caaaagagat gttgagttca tcagttagag tatttaaggc tatttatgat
    11521 gaaaattatg gtggttttgg taatgctcct aaatttccaa gtcctcagaa tcttatgttt
    11581 ttaatgaaat attatagtat agaaaaagat aaagatgtgt taaaaatggt ggaaaaaact
    11641 ttagatggaa tgtatagagg aggcctgttt gatcatattg gatttgggtt ttcaagatac
    11701 tctactgata aaaagtggtt ggctcctcat tttgaaaaaa tgctatatga taatgctatg
    11761 cttactatag cttttttaga tgcgtataaa ataacaaaga aagagttata taaagaaatt
    11821 gctataaaaa ctatagatta tgtagttaga gagatgaagg ataaagaagg tgggttttat
    11881 tcagcacagg atgctgatag tgaaggtgaa gaaggtaaat tttatacatt taatcctctt
    11941 gagataattg aagttttggg tgaagaagat ggaattttct ttaataatta ttttgatata
    12001 actagtagtg gaaattttga aggcaaaagt attcctaatc ttataaaaaa taaagaatat
    12061 gaaagacata atgaaaagat agctgacctt agtaaaaagg tatttgaata tagaaaagaa
    12121 agaacatctt tgcataaaga tgacaagata cttacatcat ggaatgcatt gatgatagtt
    12181 gctttaacaa aggcatatag tactttaaaa aatgacatat atttagagta ttcaaataag
    12241 tgtcttaact ttataaataa taatcttgta aatgagtcag gtagattatt agctagatat
    12301 agagatggaa gttccgacta tttagcatat ttagatgatt atgctttttt aatctgggcg
    12361 tatatagagc tttatgagtc aacatttaat atgaaatatt tagaaaaggc tttaaatttg
    12421 aatgaaagct gtatcaacct tttttgggat tatgaaaaaa gtggatttta tatatatggt
    12481 aaagatagtg aaaatttaat agcaagacca aaagatttgt atgatggagc tattccatca
    12541 ggaaattctg ttcaattata caatcttata agacttgcga aaattactgg agataataga
    12601 cttgaagaaa tgtcatataa acaattaaaa ctgtatgtgg ataatgtaaa gagttctcca
    12661 actggctaca gcttttatat gctttcattg atgtttgaac tttattctac taaggaaatt
    12721 atatgtattt ttaaggaaga ttctgattta attgcattta aagaattgat aagtgaaaat
    12781 tttataccaa atgcaacttt tttagcaaag aaatacaatg aagaaaacac tattataggt
    12841 tttttaaata actatatatt gaaggatgat aagactagtt attacgtgtg tcaaagtaat
    12901 agttgtagtc aaccaattaa tgatttacaa aaattaaaag atatgatttt aggagtagaa
    12961 taatcataat agcgaaaaag gagatgcttt aaaataaaag tctaatcatc tccttttttt
    13021 gtataaaaaa cagaaaaaga gttcctaaga acacctaatc tatgtaatga ttgttttgtt
    13081 aaaaaataaa aatgacatta tagttatata aaatattgta ctataaaaaa gtggtattat
    13141 agatgcgata gtattaatct tttttattta ctctatttct tctttttgca aattcttcaa
    13201 gtgcttcaga tttaatttta tctatttctt catattccat aaaattagca tcttcttcaa
    13261 agccatcttg ttcttcacca ttaaaacgat acattttttt tagtaaactc cataaggtct
    13321 ctaattcatc tttggtaaat tcgtgaaata catctgccat aaagtttatt ccagtcctac
    13381 tacatgtcac cataaccttt tttcctaagt ctgtaacttt aacattaatt gctctcttat
    13441 catgaggact tggaattaca tcaacatatc catttttttc aagattagca actagtctat
    13501 ttatattttg tttacttgta cccatttttc ttgcaatatt atttaatgtt gtttcttcct
    13561 ctggtaaatg aagaattgat aaaatagtca tatactgtct tgaagtaagt ataccaaaat
    13621 atttatctcc ttctatttgt attttgtttg taagggtaaa aagagttgca tatgtttgat
    13681 tcattaaaaa gagctctttt agttcatttg aataatccat gaaatatatc tcctttattt
    13741 ttagatgttg ttttgtcgaa aacagaatat catgaattaa ttctaatgtc aatatggtgt
    13801 caaaatatat taaagtatta cttttttatg taactattaa ttaattaaat tattgtataa
    13861 ttttatgtaa aaacttctat gtaccaatga taacttctat gaggaattaa taattcttaa
    13921 attttttaga aagattttga cttgaaaata cttgtaatat aatatataaa ttaatatact
    13981 gaataaaatc aatctggagg gataattatg aagtgtacaa actttacttt caaaggagag
    14041 gaaggtttag atatatatac atataaatgg gaagatgaaa atataaaaaa accaaaggca
    14101 gttatccaaa ttgcacatgg aatggctgaa actgcacaaa ggtatgagac ttttgccaag
    14161 gtacttacta aaaatggtta tatagtatat atcaatgacc atagaggtca tggaaaaact
    14221 gcaaagataa tagaaaatgt tggacattta gctgaaaaag aaggatttag atgtcttgta
    14281 gaggacatgt atactttaac aaatattata aagaaagaaa atgaagattt accaatttat
    14341 ctatttggac acagcatggg ttcatttgca agtcaaagat atataatgga ttacagtaat
    14401 aatttatctg gtcttatact atgtggttca aatggaaagc aaggaatcat tctaaatctt
    14461 gctcatctga taatcaatcg tgagattaaa aagtatggaa gacgttctaa aagtaataaa
    14521 ataaataatt tgatatttgg cggcgaaata ataagaagaa atgaaaagac taagtttgac
    14581 tggttaagta gagataaaga acaagttgaa aaatatataa atgacccatt ttgtggagta
    14641 gtgtgtagtt gtggcttttt ttatgattta gttcaagggt taaaagaaat tgaggataaa
    14701 gaaaacttaa aaaaagtgcc acttgatata cctatttaca taatatctgg agataaagac
    14761 cccataggaa aaaatggtaa aggagtctta agactaaggg atagatatat aaaattagga
    14821 gtaaaagatg taacttgcaa actttacaaa gatggaaggc atgaattgtt gaatgaaata
    14881 aatagagaag aagtttttga ggatataatt tgttggttaa ataataaaat agaaatattg
    14941 taataaaaaa atataatgat atatttgctt taaatttatt tatatataat atttgaaaag
    15001 tggattatgt atatgcaaag tagaaaagag agagcccaga agtgattttt tgctataatg
    15061 ccatttattg aataggtggg tgttattata aataaattat taacactcag tattattatc
    15121 attgtatatg gaatttattt tatttaaaat tccttgaaag tttaaatata gtaagttgta
    15181 gtaaagagaa aatataaata tttaaatgct atttaatgtt aatctattaa ttaggttata
    15241 cttaaactta attaatagat tttatatttc aagacaatga gatagggttt tagattaaaa
    15301 tataaccaat ggttaaatta cagacatttt gttaactttc cctcgattta ctagtaaata
    15361 ttttttttat atacttaaat tacaacagat gtactaggga ggtatatatg caaattaaaa
    15421 taggtaaagt catacagtgt cttagaaaag aacgaaattt aactcaagaa caattagcaa
    15481 agtttatagg tgtgtcaaca ccagcagtat ctaagtggga gagtggaaat tcttacccag
    15541 atatagaatt attgccttta ttagcagact tttttaatgt gtctattgat aagcttttaa
    15601 attacaagat tgatttaagt gaagaagaag taatgaaaat ttataaagaa ttagaatcag
    15661 gctttgctag aattgaaatt gatttatcaa cagaagaacc aaaagaagaa tttagacaag
    15721 acttagaatc tgtaaaaaag ctttccaata tgtacataga gaagtatcct aaaagctatt
    15781 tattgaagct aaggatatgt tcgctgtatc agatgtattc atataaattt ggtaagagtg
    15841 aacttaatga cagagtaaaa gaaacaacga atattcttga agatatagtg agaaatacag
    15901 atgatatcca aataaaagaa acagctctta ttatattatc aaacgcatat tgtatgttgg
    15961 aggattatga aaaggcagag ttgtatttaa atatgataca taagtcaata ggggatacaa
    16021 gtgttaattt agctatgata tatttaaagc aaaatagact tgaagaagca gaaatattgc
    16081 ttcaaaataa attgtttagt aatgtattta atataagcat ggattgtaag ggaataatta
    16141 atgtatacaa aaatcaatac aaggaattaa agaaaaaatt ggagaataga aattttaata
    16201 aaaatgcaat tgagagagaa atggagtata taaaaaataa gttgctgggg tatgcaaatc
    16261 tttctttaga aataaaaaaa atgctcagtg aagataaagg tgcatttttt agtatgtata
    16321 tggattatat ggaattatct ctaatttttt tattttttaa catgaaagaa gaagcaaaaa
    16381 aagccttata tagtctaaaa gaaatattag aaaaatatcc tatacatgaa aatttggatg
    16441 taagtcaaat gagatttttt gataaggtag agtctaaaaa tttatatact tttaatatat
    16501 atacaaactt gttaattgta cttaatgatg atagttataa tgagcttaga gaagaaccaa
    16561 tttttaagga tgtaattgaa aagattttag atatggaaaa aatgttgaaa aataaagaat
    16621 aagtaaaaat ttaatatagg acttaaagat taatcaaatt aaaagaaagt gtttcaaaat
    16681 aaaaatttga atcacctttt ttgtcggtct taaacagtta tttagaaaaa tagaggtttc
    16741 aaaatagaaa tctgaattat ctgtattatt acacaaaaat agaaacctaa atttataaaa
    16801 atagaataaa gtttgttata taaaatgtaa ttttgtctat aaaatctcac ctgttttctt
    16861 aactttaagc gtgaaaaaga ggatgtatca tgaatttttt agttcatgat gcatccttct
    16921 ttgtatgaaa tccaatatat ctttattatt taaacaatgg aaaagagtct tatttatatt
    16981 ttgagacatc tcttttattt aattatgaag ttttatacag ttcaataaat aggaattata
    17041 tttagaaact catgacatta tttcttagat agtgattaaa tttatcatat tccattttag
    17101 ataacttttc tgggtaagac attacaatca ggttatgccc attaaatttt tcatggtatg
    17161 gtggatgaat gtgttcataa ttatttgtct tatattttac tctttcatag aacgccttgc
    17221 gataaattga aatctcatca attggaaggt caatttctaa aataatagtt tttcctttct
    17281 tatttaaaag ctctaacgct ctttgcccat atctattatt gcgcatttct ggaaaaatgc
    17341 agaaatgttc tacatagata aagttatctg tttcccaaca taaaattatg ccgacaaact
    17401 ggtttttatc ataaattaaa ttgaattgat attctttatt tttcaaaatt tctgcttgca
    17461 aagaagattt tctttgttca tgaaatggga aactaatttt atataattcc atagccttgt
    17521 aatataattt attttcactg gttcttaacc gttcaaattt cactttactc ctcctaatca
    17581 ctaaaattcc aatttattta tgagttcatt ttaccatagt cagtatttgc atacaatgtg
    17641 ttttaagttg agtgtcattt ctttctttta tttatgttta tatctcatag actaagagat
    17701 aattacttaa gacataacta aaaactatta tattaagctt tgataatcta aaaacagtat
    17761 atttatggac ttttctaggt ctaaaagtaa tcttaataat tttagaagtt acaaaaaagt
    17821 tacataaatt tggaaagcta taatatacat atataatata aggttaaaac tgtaatgaat
    17881 aatattaatt taaaatttga acatggaagg agattat
//
LOCUS       DAEMHZ010000076        17914 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000076 DAEMHZ010000000
VERSION     DAEMHZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.076"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            60..974
                     /gene="murB"
                     /locus_tag="KPK72_002378"
     CDS             60..974
                     /gene="murB"
                     /locus_tag="KPK72_002378"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG2489586.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            978..1691
                     /locus_tag="KPK72_002379"
     CDS             978..1691
                     /locus_tag="KPK72_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2489587.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1722..2579
                     /gene="rapZ"
                     /locus_tag="KPK72_002380"
     CDS             1722..2579
                     /gene="rapZ"
                     /locus_tag="KPK72_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG2489588.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2591..3697
                     /locus_tag="KPK72_002381"
     CDS             2591..3697
                     /locus_tag="KPK72_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG2489589.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3766..4191
                     /locus_tag="KPK72_002382"
     CDS             3766..4191
                     /locus_tag="KPK72_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2489590.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4208..5155
                     /gene="whiA"
                     /locus_tag="KPK72_002383"
     CDS             4208..5155
                     /gene="whiA"
                     /locus_tag="KPK72_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG2489591.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5312..8881
                     /locus_tag="KPK72_002384"
     CDS             5312..8881
                     /locus_tag="KPK72_002384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG2489592.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9090..10049
                     /gene="pfkA"
                     /locus_tag="KPK72_002385"
     CDS             9090..10049
                     /gene="pfkA"
                     /locus_tag="KPK72_002385"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2489593.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10101..11861
                     /gene="pyk"
                     /locus_tag="KPK72_002386"
     CDS             10101..11861
                     /gene="pyk"
                     /locus_tag="KPK72_002386"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG2489594.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12036..13394
                     /gene="rlmD"
                     /locus_tag="KPK72_002387"
     CDS             12036..13394
                     /gene="rlmD"
                     /locus_tag="KPK72_002387"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2489595.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(13867..14505)
                     /locus_tag="KPK72_002388"
     CDS             complement(13867..14505)
                     /locus_tag="KPK72_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489596.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMSSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEADFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(14915..15091)
                     /locus_tag="KPK72_002389"
     CDS             complement(14915..15091)
                     /locus_tag="KPK72_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489597.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(15145..15241)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16037..16438
                     /locus_tag="KPK72_002390"
     CDS             16037..16438
                     /locus_tag="KPK72_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG2489598.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLISIALKPLAQPPFPLERIIEKLEKEETE"
     gene            16435..17502
                     /gene="rsgA"
                     /locus_tag="KPK72_002391"
     CDS             16435..17502
                     /gene="rsgA"
                     /locus_tag="KPK72_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2489599.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVYRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
BASE COUNT         6989 a         1986 c         3331 g         5608 t
ORIGIN      
        1 ctaatgtaat ttaaataata atgacagcta aagttcagta taatttggga ggaaatacaa
       61 tgaataatca agatatatat ataaatttat taaatatatt aagtaaagag gatataaaag
      121 tcgatgaacc tatgaaaaaa catatttcat ttagggtagg aggaccagca gatatattag
      181 ttagacctag aactgaagaa caattaaaaa atgtacttaa gttggtaaaa gaagaatcta
      241 ttccgtattt aataattgga aatggttcaa acattttaat taaagatggt ggaataagag
      301 gagtggtaat agaattagct gataatttca attcttatga gataaatgat actaggatga
      361 ctgctcagtc aggagcttta ttatcagttt tagggaaagc tctccaaaag caagaattaa
      421 aagggtttga gtttgcatca ggaatacctg gcacattggg aggagcatta gccatgaatg
      481 ctggagcata tggtggtgaa atgaaggaca tagtgaagtc agtaagactg atggatatgg
      541 aaggcaatat atttgaatta tcaaatgaac agatggaatt tgggtataga aaaagtataa
      601 tatcgaaaaa cggatatata gctttatcag cagaattaga attacaagaa ggaaattatg
      661 atgaaataaa aagtttaatg gatgatttag cgacgagaag aataactaaa cagccattaa
      721 attttgctag tgcaggaagt acatttaaga gaccaacagg atattttgca ggtaaattaa
      781 tagaagaaac tggattgaga ggtcttactt taagaggagc acaagtatct gagaagcatt
      841 gtggatttgt agtcaatcaa ggagaagcaa gtgctaaaga tattttggat ttaatatatg
      901 ttataaaaag tgctgtatat gccaagtttg gggttatgtt ggaagaagaa gtcaaaatac
      961 ttggagagga ttaacaaatg aaaatactgg ctgattatca tacacataca ttatatagtc
     1021 atggcaaagg gactattgag gataatgtaa aggtagccat ttctaaagga ataaaaacaa
     1081 taggaatatc tgaccatagt tataaacata ttgcatatgg tgtaaagaaa aaagatattc
     1141 tcaaaatgag agaagaagta gattatttaa atgataagta cactgatata aatatacttt
     1201 taggtatgga gtgtaacata ttagatgata aaggaaatat agatatggat gataaattta
     1261 ttagagattt tgattatatt atggctggat atcattttgg ttctactcca acatcattaa
     1321 gaagcttatt aaatcattgt aataactata tttttaaaac tgaaaaagca aaagaatata
     1381 atacaaaagc tgtaataaat gcgataagta ataatgatat ttttataatt actcatccag
     1441 gagataaagg cgatgtgtat atagaagaag tagcaaagac agcaaaaaag acaaatacta
     1501 gacttgagat aaatagtagc cattcttttt tgaatgtaaa tcaattaaat aaaataaaag
     1561 ggctagaaaa taaatttata ataggctcag atgcacattg tccagaaaga gttggtgatt
     1621 ttgaactagc tatgaagact atatctgact ctaatctgga tatatcgtta gtagaaaaca
     1681 ttataatata ataaacataa gataaaaggg aggagttagc aatgaaattt gttatagtta
     1741 caggtctttc aggatctgga aaaagtgaga caatgagagc tttagaggat atgggatttt
     1801 attgtgtaga taatctacct cctgctttga taactaaatt tgcagaatta tgttatcaac
     1861 caaattcaag tattgataaa gttgctctag ggatagatat aagagggaga aagttttttg
     1921 aagcgcttca tgaaagctta aattatttag aaaaagaaaa ttatgaatat gaaatggtat
     1981 atttggattg taatgatgat gtattgttaa agagatataa aatgacaaga agaaatcatc
     2041 cattggcaaa agatatgcag attccagaag gtataaaaat ggaaagaaag ataatggaac
     2101 ctttaaagga gttatctaca tgtataattg atacaactaa catgaaacct aaagacctta
     2161 aggaagaaat aaagaaaata tattcttctg gtgaagataa tccaaaccta actatttctg
     2221 tagtatcttt tggatttaaa catggtatac ttgctgatgc agatttagta tttgatgtaa
     2281 gatttcttcc aaatccctat tatgttgaag agttaagagc taaaactggt gatgataaag
     2341 aagttagaga ttatgttatg aattctaaaa tcagtgaaga attttatgtg aaacttttag
     2401 atatgattca ttttttagta cctcaatata tagaggaagg aaaacaacat ctagtaattg
     2461 gtgttggatg cacaggtgga agacataggt cagttactat aacaaatctt atagcggagg
     2521 atttatctaa taagggatat agagttgtaa agaaacatag agactctatg ttgcgataat
     2581 ggaggcaaac atggctaaat tacttattat tgtaggaatt ttaggttgga ttgcattaat
     2641 tgtatcgtta tttatatata aaaaagcaaa aaatgaaaaa gtaaaactat ttcaaaggtc
     2701 tattgttgaa aatagagaac caaatgtggt tgtgataggt ggaggtactg gtcaatcggt
     2761 atttttaaga ggtcttaaac acactacgca aaatataact gcaattgtaa ctgttgctga
     2821 tgatggtgga ggttcaggtg tattgagaga agacttagga atgctcccac caggagacat
     2881 aagaaactgt ttattagcac ttgcaaatat agagccaaca atgaatgaag ttatgcaata
     2941 taggtttaca gaagggcttt taaaaggtca gagttttgga aatttgtttt tggctgctat
     3001 gaatgggttg tatggaaact ttgaaaaagc tgtatataaa ttaagtgaaa tttttgctat
     3061 aacaggaaga gttttaccag taactttgga agatgtaaat ttagttgcca aattagaaaa
     3121 tggcaatata ataaatggag aatctagtat acctgaagag tcaaaaattc aaaaaagctc
     3181 tattgataaa atttttttaa atccaaaaga tgtaaaacca ttaaaggatg ttattgcatc
     3241 tatttatgat gctgacatta taattatggg tcctggaagt ttatatacaa gtataatacc
     3301 caatttactt gtagagggga ttgtagacgc tattaaatcg tctgtagctc caaaagtata
     3361 tatagcaaat ataatgactc aaccaggtga gacagaaggg tataatgtat tagagcatgt
     3421 taatgctata gtaaaacaca ctgatgagaa tttaatagat tatgtaatcg caaataatga
     3481 gatattgcca gaagaaatgc ttgacttata taaacaagat ggagcagagc aagtcttgtt
     3541 agataaaaag caaaaagata aattgaaaga gatgggtata aaaactgtag agaagaattt
     3601 aattgaaata aaaaataatt atataagaca tgatgcaaag tatatatcta acatagttat
     3661 tgagttagcc ctaaatcata attacaacaa aagttagtaa aaaaggattt taaaaaattg
     3721 tgtagaaatc cttatcatta tgttagtttt aaggagtgaa catcgatgag agaagaggtt
     3781 agtgctggtg gtgtagtcct gtttggcaat acgattcttt tactaaggaa gttcaatgga
     3841 gattgggtgt tacccaaggg aaaagtagaa gaaggggaaa ataaccaaga ggcagcatta
     3901 cgtgaagtaa gtgaagaaac tggagtaaaa gcagatatat tgaaatattt aggtgaaata
     3961 cactatacat tcaaagaaaa ctgggatgaa aatagagcag ttcataaaac agttttttgg
     4021 tatttgatgc aagcaaagaa tatggatact attccacaaa aagaggaagg ctttattgat
     4081 gctaaattta tccacctaga tagagtagta gacttagcta ggtatgatga tgaaaaggag
     4141 ataataaagg ttgccttaca agaaataaaa aaaaggttaa agaaaaatta gttaataggt
     4201 gatagatatg tctttttcaa ctgaaaccaa aaatgaacta gcaagagtta tatctgggaa
     4261 tgaatgttgt aatatagctg aattgtcagc tctagttaaa tcagggggaa gtatacaaat
     4321 tgttggatat aagaagctaa atctaaagat aacaacagag ttaaattcaa tagcccgtaa
     4381 agtatttaaa ttattaaaaa agaattttaa tatcaatact acaatctcag taaataagaa
     4441 tcaaatgcta aagagaaata acagttatgt attaatggta acaagcgaaa tgggttcaga
     4501 aatgttatta aaaaagttag gtatacttga agataaagaa ggatttttta ctataaacaa
     4561 aatttcagag aacttaatta agcatgaagg atgtaagaga gctttcataa gaggagcttt
     4621 tcttggtgga ggttctataa gcgacccaga aaagaactat catatggaat ttgtaactaa
     4681 taatgaggag tttgcagatt ctctaaaaga acttataaac tctttaggtt ttaatagtaa
     4741 aattgtggct agaaaaaata atcacgttgt ttatatcaaa gagagtgaac aaatatcaga
     4801 tttgttgagt attataggag gacaccatgc tcttttaagt cttcaaaata ctaaaatagt
     4861 aaaagaaatg agaaataatg taaacaggat agtaaattgt gagacagcta acctttctaa
     4921 aacagtaaat gcagcagtaa gacaagtaga aaatattaag ctcatacaag agactatagg
     4981 aattagcagt ttgccagaaa atttacaaga gatagctaag attagaatcg agtatgagga
     5041 tatgactctc aaagaacttg gagagatgtt agaaccacca ataggaaaat ctggagtcaa
     5101 tcatagactt agaaaaatag aagaaattgc aacagatttg aaaaaaaaat catagcaata
     5161 aattgagggg acaatctcag aataagcatt aatttataac atataatggt atacgttgaa
     5221 gttaattcct attttgagat aaaagtctct gtttttgttt tagtacatat atttgtggta
     5281 aaattaaaat tataaagcag gaggtgattt aatgaacaaa gattttgctc atcttcatgt
     5341 acatactgag tatagtttac ttgatgggtt ttcgagggtg aaaaaactta taaaaagggc
     5401 taaggaacta aacatgagct caatagctat aactgaccat ggttgtatgt ttggtgttat
     5461 agatttctac aaaacagcta taaaagaagg aataaagcca attatagggt gtgaagtcta
     5521 tacagctgca cgtggattga gagataaaga cccaaactat gacaaatatc aaggacattt
     5581 agttctgtta gctaagaata tggaaggata taaaaattta ataaaaatag tttcaacttc
     5641 atacgtagaa ggtttttact ataagcctag agttgacatg gaagaactta aaaaacataa
     5701 caaagggatt atagcacttt ctgcatgcct tgcaggagat gtagcacgtg ctttaatgaa
     5761 tagaaattat gaaaaagcaa aacaatttgc tcttgactac agagacatat ttggagaaga
     5821 aaatttcttt ttggagattc aagatcacaa tttacctgag caaaaagaag taaactctgg
     5881 tttagtaaaa ttatcaaaag agactggtat accactagtt gctactaatg atgttcacta
     5941 tgtaaataaa gaagattcga aaatacatga tgttcttatg tgtattcaaa tgggaaaaac
     6001 attaaatgac ccaaatagaa tgagatttgg tagtgatgag ttttatttaa aatcaagaga
     6061 agaaatggaa gaattatttc catatgcatt agaggctatt gacaatactg tgaaaatagc
     6121 tgatatgtgt aatgtggagt ttgattttaa tactatacat cttcctaagt atgatgttcc
     6181 agaaggatat acaccagaaa cttatttaag ggagttgtgt tttaatggat taaaagagag
     6241 atatgataat cctagcaatg agatactaga gagattaaat tatgaattag atgtaattga
     6301 aaaaatgggt tatgtagagt attttttaat tgtttgggat tttataaatt tttctaaaga
     6361 aaataatatt atagtaggac caggtagagg tagtgcggct ggttcgatag tagcatatac
     6421 acttaaaata actgatatag acccaataaa gtactctcta ttatttgagc gttttttaaa
     6481 tccagaacgt atatccatgc cagatataga tatagatttt tgttatgaga gaagagaaga
     6541 agttatagat tatgtaaaga gaaaatatgg tgatgaccat gtagcccaga taataacttt
     6601 tggaacaatg ggtgcaaaag ctgcaataag agatgttgga agggtattgg atataggcta
     6661 caataaagtt gacaaggtag ccaaggaaat accatttgct ttaggaatga caatagataa
     6721 ggccctagat acaaatccta atcttaaaga attgtatgac caagacccgg aaacaaaaga
     6781 aataataaat atatcaaaac agatagaggg aatgctaaga catgcttcta ctcatgcagc
     6841 aggggttgtt atatcaaaaa atcccgtaga tgaatatgta cctttgtaca aacatcaaga
     6901 tgcaatcaca acacagttta ctatgacaac acttgaagaa cttgggcttt taaagatgga
     6961 ttttcttggg ctaagaactc ttacagttat aagagatact cttgatttaa tagaaaaaaa
     7021 tagagttata aaaaattaca cagaaaaaat agacttttca aagatggatt atgacgaccc
     7081 gaaagtatat gaaatgcttt catcaggaaa tacactaggg gtgtttcaat tggagagtgc
     7141 tggcatgaga agttttatga aacagctaaa accagacaat tttgaagata ttgtagcggg
     7201 aatatcccta tttagaccag gtccaatgga ttctatacct gcatatataa aaaataaaag
     7261 caacccaaaa gatgtaacat atctacatga aaaattaaag cctataatgg aagttacata
     7321 tggttgtttg gtatatcaag agcaggttat gcaggtcgtt agagatttag gtggttatag
     7381 ttacggacga agtgacctag ttagaagagc catgagtaag aaaaagatgg acgtgatgga
     7441 agaagaaaga caatatttta ttcatggtaa atttgatgaa gatggtaata tagaaattgc
     7501 agggtgtatc agaaatggtg tagaagagga aatagctaat aaaatatttg atgatatgat
     7561 agacttcgct agatatgcat ttaataaatc acatgcagct gcatatgggg ttttagcata
     7621 tgagacagct tatctaaaaa catattatcc agttgaattt atggcagcat tgataaccag
     7681 tgtaatggga aatactgata aggtagttga gtatataaga gaatgtaaag cactagaaat
     7741 agatgtatta cctccagata taaacaaaag tttttcaaag ttttcagttg aaggcgacaa
     7801 tataagattt ggtctagcag ccgtaaaaaa tgttggcgta aatataataa acaatataat
     7861 cgctgaaaga gaatctaatg gattatttgt agatttagta gatttagtca aaagattaga
     7921 ccaaaaagat acaaataaga gagtaataga aagtctaata aaatgtggag cttttgataa
     7981 tataagtgaa aatagagcta gtctgatggc tggttatgaa agtttattag aaagtgtatc
     8041 tatggataga aagaaaaatg ttcaaggtca aatttcttta tttgatgctt ttggagatag
     8101 tgaggaagat agtgattttc aacaaatata cagtttacct aaaaggcaag agtatgaaga
     8161 aagagagaga cttaaccttg aaaaagaagt cttgggaatg tatgtaagtg gacatccttt
     8221 atcacaattt gaaaaagaac tacaagaaaa aacatctatg gataatggaa aattaaattc
     8281 attaaaagag gaagaagagt catacctaca aatgaatgaa acagatgtta taatgggtgg
     8341 aatgattgta actaaaacca ttaaaacaac aaaacgaaat gaaattatgg catttattga
     8401 attagaagat ttgtatggtg ctatagaaat aatagttttt cctcaattat tacaaaagta
     8461 taatgtcatt ttgaatgaag ataatataat atatgtaaaa ggaacattaa gtataaaaga
     8521 aggcgaaaat gcaaaactta tagctagaga gataaaagat ataaatgatg atactgactt
     8581 tgcagataga aactacaatt caggaagaac tagaaatgta gaaaatatta agccagaaag
     8641 tggtaaaaaa ttgtatttga aaatagatag tatgtctgat actgatacaa ctaataatat
     8701 tgtagaaata gccaaacaat atccaggtaa tgacagtata tacttgtacc ctatggatga
     8761 aaatataaat ggtaaacgta aagcatataa aatgaccggg gcattgactt gtatatgtga
     8821 tgaatttata aataacttag aagagttact tcctaaagat aacataaaag taaaagcttg
     8881 attttttata taaaaatcta aatatattat aataatatga aaaatattgt ccgttttgtg
     8941 atataatatc acaaaatgga catttaagga catttaattt ttttagtctt gatgggatta
     9001 aaaagtgata tagggacaaa aaaagtccat tagaatacaa taacaatgat tataataaaa
     9061 aattgacata aaatccaagg aggtaaaaaa tgaaaactat aggattacta acaagtggag
     9121 gagatgctcc aggaatgaat gctgcgatta gagcggttgt aagatcagca atatattatg
     9181 gatgcaaagt atatggaatc aatagaggat ataagggatt actagaagaa gatttaactg
     9241 aaatgaactt atcttctgtt ggagatatta tacacagggg aggaactata ttaaaatctt
     9301 ccagatgtga agaatttaaa acagaagaag gtagattaaa agcagtaaaa attcttaaaa
     9361 agtataaaat cgattgctta gttgtaattg gtggggatgg ttcatttgca ggagctcaaa
     9421 aacttagtga cttaggattc ccagctatag gcataccagg aactatagat aacgatttag
     9481 catatacaga ttatactata ggatttgata ctgcaatgaa tactattata gatgctatag
     9541 gtaagataag agatacatca tcatctcatg aaagagttaa catagtagaa gttatgggaa
     9601 gacactgtgg agatttagcc ttatatgcag gtcttgcagg aggagcggaa actataatag
     9661 taccagaagt agaaattaca gtagatgaag ttgctctaag attaaagact actcaaaaaa
     9721 gaggtaagag acatagtata attgttttag cagaaggagt cggaagtgct agtgatttag
     9781 aaaaggaact taaaaaagag tcaggagcag atttaagagt cactgtattg gggcatgtcc
     9841 aaagaggagg aagcccaact gtatctgata gaatactagc aagtagactt ggagtaagag
     9901 cagtagaatt attattagat ggaaaatcag ctagagttgt aggaataaaa gaaaacaaaa
     9961 taattgattt agaaataagt gaagctttag ctcaaaagaa agtatttgat aaagaagctt
    10021 atgagatggc aaaaatatta tctatataag ttttagtatt aaaaataaat ctgtggaata
    10081 atatctcagg aggagcaaat atgttaaata atgtaaagaa gacaaaaatt gtttgtacac
    10141 taggtccagc atcacaaagt gaggaagttt taactcaact tatgcaaaat ggtctaaatg
    10201 tgtgtagatt taacttttct cacggttcgc atgaagaaca taaagaaaga attgatatgg
    10261 caaagaaagt tagagaaaaa ttaaataagc cagttgctat attattagat actaaaggtc
    10321 cagaaattag aacaggtaac tttgaagacc cagaggtatt tttagaagaa gggcaaaagt
    10381 tcactataac tatgaaagat attatgggga ctaaagaaat gtgtacagta agctataaag
    10441 gtcttgctga agacgttaaa tcaggagatt ctatactaat agatgatggt ttagttggct
    10501 taagagtaaa agaaataaat ggagaagata tagtttgtgt tgttgaaaat tcaggaatag
    10561 ttaaaaatca taaaggagta aatgtacctg gtgttaagat aaatttacca gcaataactc
    10621 ctaaagatat aagtgatata gagtttggaa taagtcaagg aatagattat atagctgctt
    10681 cttttgtaag aaaggcatct gatgtattag caataagaga agtattagag aacaataatg
    10741 caactgatat acaaataata tctaaaatag aaaaccaaga aggtgtagag aacctagatg
    10801 aaatattaaa agtttctgat ggtataatgg tggctagagg tgacttaggt gttgagatac
    10861 caactgaaga gatgccaata gttcaaaaaa tgatgataaa aaaatgtaat gaattagcaa
    10921 agccagttgt tactgccact caaatgcttg attctatgat aagaaatcca agaccaacaa
    10981 gagcagaagt tacagacgtt gccaatgcta tatatgatgg aacagatgcg ataatgttat
    11041 ctggagaaac tgctgctgga aaatatcctg tagaggcagt aaaaatgatg gctacaatag
    11101 caaaaagaac agaagagact ttagattatg atagattatt aaaggaaaat ggtactaaca
    11161 atgttactgt aacagatgca ataagccatg caacttgtac aacagcagta gatttaaatg
    11221 catcagctat aataacatct acttcttcag gatatactgc tagaatggta tctaaattca
    11281 gaccaaaatc tccaataata gctactacaa ataatgaaaa gactatgaat aaattagctc
    11341 ttacatgggg agtatatcca gttaagtctt ctgttgcagg aaatacagat gaagttatag
    11401 aaaaggcaat tgaggcagct agacaagctg attatataga taatggggaa ttagtagtta
    11461 taactgctgg agtaccagta ggagtaagtg gaactactaa tttaataaag gttcatgtaa
    11521 taagcgaaga aatcgtgcaa ggtataggtg taggtactca aacagtagaa ggtaaagttc
    11581 gtataataaa gaatggaaat gcttgtgtag actttaatga aggcgacata cttgtttcta
    11641 ctgctactga tgctgaaatg aacaattata tagaaaaagc aggagctgta gtaactgaga
    11701 atggtggaat gacatcacat acagctattg taggtataaa tttagatata ccagttattg
    11761 tgtctgcagc tgatataaca aatctagtaa aagatggaga agttgtaact gtggatgcat
    11821 caagaggcgt ggtatataga ggttcaacta gagttttata agaaaaataa taaatttatt
    11881 tactaagggg ctttataagc tccttttttt ttggtaaaat aaaatattgt agcttagtaa
    11941 aaaatatagt ttaattaata gcaatatttt aatataaatt taaagaaaaa agtttgtaaa
    12001 gatttaaata taatagattg attggagggt gatttatgct ttcgaaaaat gagatgtata
    12061 ttgtagatat agtagacatt ggtcaaggcg gagtaggagt aggaaagtac aatggtatta
    12121 cagtttttgt agagggcgga ttaatacatg ataaagttaa agttaggatt aataaagtaa
    12181 agaaaaatta tgctgtggga gatattgttg agataataga aaagtctcca ttcagggtga
    12241 aaagagtgtg tagtgacaat cttagggatt gtggagggtg tcagatacaa gacttagatt
    12301 ataataaaca acttgaaatg aaaacaaatg aagttaaaca agtactttcg agaataggta
    12361 agttagagga tgtagaagtt catgacacta taggaatgga taatccatat agatatagaa
    12421 ataaagcaca atttccaata caaaagaata atggtattcc aattattggc ttctataaaa
    12481 agaagactca tgatgtaata tcgacagaaa aatgcgtaat acaacatgag ataaatgata
    12541 aaattgttaa aataataaaa acatatataa gggcctataa ggtaagtata tatgatgaaa
    12601 agactcacac ggggttgatt aggcatttag taacaaaaat aggttttaca actaaagaag
    12661 taatggttgt attagtagca aatggaaaca aacttccata tcttaaagaa ttagcttcag
    12721 tgcttaagga aaatatacct ggatttaaga ctttggttgt aaatgtaaat caaaaagata
    12781 cgaatgctat tctaggaaga gaaaatatag ttatatatgg agatggaaaa ataaacgatt
    12841 atataggtga cttagtgttt gagatttcac cattatcatt tttccaagta aaccctgtcc
    12901 aaactaaagt attgtatgat aaggctcttg aatatgcaaa tttaaatgat actgatactg
    12961 tttttgatat ttactgtggg ataggtacaa tatctttatt tttggcacaa aaggctaaaa
    13021 aggtttatgg aatagaaatt atagatgatg ccataaaaga tgctaaaata aatgctaaat
    13081 taaacaatat acataatgct gaattttatg ttggaaaggc agaggaaatt gtacctaaga
    13141 tgtatagaga gggtaagact gccaatgtag tagttgtaga cccacctaga aagggatgtg
    13201 acgaaaaggt actagagact attgtttcaa tgaatccgga aagaattgtc tatgtttctt
    13261 gtaatccttc tactttagca agggatttag actatctaaa tgttcacgga tttaaatgtg
    13321 ttgaggcaca acctgtagat atgttccctc attctacaca tgtggaaact gtagctaaga
    13381 taatcaaaca atagtaatgt ttatatagat tatatactaa taatatattg tatttgccac
    13441 cgatttgcca ctgtaaagcg attttggtgg caaatttagt ctacaatgtt atcaaaaatc
    13501 tctaaagttt tatttttaat tctataaaac ttattataat tgtaatttta aaagaaatag
    13561 tcaatctaca gtagccagat tttatacaac ttattttttt catagagtaa tttacataat
    13621 ctatagttga atattattta atatttttat aattcattaa caaatatagg cgttacaaat
    13681 gttgataaac atgtgaaagc acatatccac ttataattat tttttttaaa accataaaat
    13741 cacctcttca tttgttgtat gtttttataa caaatgaaga ggtgatttcc agacaattta
    13801 aaaggttttt gtatattgac taaaatttat ttttattatt tataattctt aataatacat
    13861 atgtacctac tgattccata ttaaactgta taatttttgt tcatcttttt ttataattaa
    13921 aactacatag ttttcataat ttctcttata tatatactta tcatcttttt taacttctaa
    13981 cttatatttt ttaaaatctg cttcaatttt atttttatta cttttattaa tgttaacaga
    14041 atctctttta aatttatcaa tatatttttc tatttcatta atattatctt tgcttacaac
    14101 tataccacct tttaaattaa aaactttttt gaaatttttt tcagaaaatc cttgaataaa
    14161 ataactttgt ccatctccat taatactatc ttgagtatca aatacttcaa tttctttgtt
    14221 aggtattggt atatcaaaat tccaagattc ctttagcatt gaaatattag ctgtttttcc
    14281 taaaaaaaga ttatatagga tacttcctaa taacaaaaca cctattaata gtagtgtgat
    14341 taatacaatt tttctctttt tcatttttcg ttcacaacct tttgaaaaaa catgatttaa
    14401 ttcatcattt gaagacatgt cttcaagaat aatttttgtt gtgataggaa tgctttcttg
    14461 aaacatatca tttgtaatat caaaatcttt tctttgatta ctcaatatcc atttcttcta
    14521 atatttcttt tgattttttc tagtacattg aatactctta tatacattct cctcttttaa
    14581 aattaaaagt tataaaagaa taagttatat aaaaaattat tatatttttt aatatagctt
    14641 atttgcaaat ttataatttt gttaatctaa tccttcaaac taaaaaatca tttatgttta
    14701 cagaatttca ttttaagaga tgtttatttt cacatagata agagaattta agttctcata
    14761 tttattattt gatacaaatt tacatcttta ttatatgtat tttaaataaa aaataaaatt
    14821 atgaatacta gaaatctatc ttaattagct ttctaacata aggctattta aagtagataa
    14881 aactaaaaag ggttctctag aaaaccaaat ctatttaatt atacttactt actaatacca
    14941 tcacttgatg caaacaagat tctaccacat tttctccgta ctctagtata aaatcatcta
    15001 gttgtgtatc tataaaatca caataatcat atgaactacc aatataagca gtcaacatat
    15061 gtgtacatct atccatttta tctctaaaca tatatattat tatcccccta aaaaactttc
    15121 ataaaaacaa aaagaatatc tctttttggc aactggctga cataactcat agtttatttt
    15181 ataagcgtta gtccctatag ctttgcgtca ctaaatttct ctagttttgc cgatttagtt
    15241 ttattctaca attacaataa tacaatagaa ttgcttatta ttcaatatac ttatttgaaa
    15301 attaaataaa tttacaaata tgaacgttat tttatatcaa aaaaatagac tatgcatgta
    15361 gaatatatgg aaaaacttga ttgtaggttt tgtatgaaaa gacagttgtt attaatttaa
    15421 gtgagataaa ttgagcaata ggcataaaat gagaaaacaa ataatagcag ttaattttta
    15481 ttattaatac taggtgaaca ttaaatttta aaataccact agattttata gaataagaaa
    15541 atgagacaaa cataaatata tgaatatatt aaattatact tgaacatact tatattttat
    15601 gataaaattt caataataat tattagtgaa ggagtgtctt gcctgatgtc agagttatat
    15661 ataatggtaa ccaacaaata aatagttgga gacgcatagg ggttctgttt aatcagaatt
    15721 tttaagtgta tttttgatac atttgattgt tgtgtcttac tctgccttta tagataacta
    15781 acattagaca acaaataata atattacata ttactttgtt aaattgtggt gtagtgggct
    15841 ttaatctgct acacttttat taattttaca cagtattatt ttgaatataa ttatgttgaa
    15901 atagaagata ctaaattttc tagtccacag gggttttctg tttttgtgta ggtaaatatg
    15961 tataccacat aaaaaggcag atataaatac ctgtggactt tttttgtaga aaatacacga
    16021 tacggaggaa ataaaaatga attataaaaa tagaaaacga gcttattatc aggaaaattc
    16081 ctgtatggat aactttattt gcaaaaattg tggttgcctt gtagttcctg aaggagctgg
    16141 tacacaacat agaaatcatt gtccacattg tttacatagt cttcatgtag atatcattcc
    16201 tggtgaccgt gctgcagatt gtgatgctaa tatggaacct attggagtgt gggtgagaaa
    16261 aaatggtgag tgggcaatta ttcatcgttg tacacgctgt ggacatctta gttctaatcg
    16321 tattgcagct gatgataatt cagtgaaatt aatatcaatt gcattaaaac cactggcaca
    16381 gccccctttc ccactagaaa gaatcattga aaagttggaa aaggaggaaa cagaatgaat
    16441 ggtatgaaac aaatcaattt acaagactat ggtttaaata ggaattttat tactcaaagt
    16501 atggaatatc atgatttata ccttgctcga attatctcgc aggaaaagaa cttttatcaa
    16561 gctgtatgtg taaatggtgt actgtttgca gaggtttcag gaaaactgcg ttttgaggct
    16621 actatacaat ctgattttcc cacagttggt gatttcgtta tgttggatcg ttgcaataat
    16681 aaaaatggta atgctattat tcatcatgtg cttcatagaa aaagcttgtt tgtgcgtaag
    16741 gctgctggaa aggtaaatga ggaacaggtg gttgcagcta atattgacat tgtttttttg
    16801 tgcatgtcac tgaataagga ttttaacatt catagattgg agaggtatct gtctttaaca
    16861 tgggaaagtg gagctatccc agtagtagta cttactaaat cagacttatg tgacaatgtg
    16921 tatcgtaaat tacaagtagt taattctata gccttgggtg tagatgtagt tgttacctca
    16981 tctatagagc cagatggtta taaacagatt tctaagtata ttaaaccagg ctgtactgtt
    17041 gccttacttg gttcttcagg agttggaaaa tcttcattaa ttaattgtct gatgggtgga
    17101 gaatttttaa atacaaagga aattcgcata gatgatgata aaggaagaca tgccacgact
    17161 agacgagagc tatttttaat ttcaggtggt ggtattgtta ttgacacacc aggtatgaga
    17221 gagttgggaa tgtgggatgt ttcagaaggt ttagataaaa gttttgcaga tatagaagct
    17281 tatttcggaa aatgtcattt tcgtaattgt actcacacct cagaaccagg ctgtgctatc
    17341 tgggaagcta ttgctcatca agaactttcg cctaaaagat gggaatccta tcgtaggctt
    17401 aaaactgaag cagtatttgc agacaataaa agcgaatact tagcaaaaaa agaaaagaaa
    17461 tttaaaaata tctcaaaaat aaataagaag attagaagat aagtgtagtt tttttgttca
    17521 ttctatgagt ctatatacta ttaatatatt aaatttgcca tcaatttgtt actttaatgt
    17581 gattttgatg gcaaatttgg tttgtggtat tatttaaagc aatttattaa tttttttaat
    17641 aaagtttgat tttttatatg atgatatata catgtgataa tgtatacata tttgttacga
    17701 taggctcctt tataaagagg tcttatgtaa gataagaaaa ggcatattta tactaataca
    17761 taatagagta tatgcttatg tatgatagag atgtttctta agagcaaaac tgcaatattg
    17821 ctaatttata aagtaaaaat atatgaatta taaaatacaa ataatatatt tataaaaatt
    17881 attgttttaa tttataataa aaattttttg ttga
//
LOCUS       DAEMHZ010000077        17893 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000077 DAEMHZ010000000
VERSION     DAEMHZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.077"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..1526
                     /locus_tag="KPK72_002392"
     CDS             153..1526
                     /locus_tag="KPK72_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2489600.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1782..2849
                     /locus_tag="KPK72_002393"
     CDS             1782..2849
                     /locus_tag="KPK72_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG2489601.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2993..3301
                     /locus_tag="KPK72_002394"
     CDS             2993..3301
                     /locus_tag="KPK72_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG2489602.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3294..4724
                     /locus_tag="KPK72_002395"
     CDS             3294..4724
                     /locus_tag="KPK72_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG2489603.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4802..5176
                     /locus_tag="KPK72_002396"
     CDS             4802..5176
                     /locus_tag="KPK72_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG2489604.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5170..7374
                     /locus_tag="KPK72_002397"
     CDS             5170..7374
                     /locus_tag="KPK72_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2489605.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDKVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7374..8783
                     /locus_tag="KPK72_002398"
     CDS             7374..8783
                     /locus_tag="KPK72_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG2489606.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIKILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8768..9832
                     /locus_tag="KPK72_002399"
     CDS             8768..9832
                     /locus_tag="KPK72_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG2489607.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9862..11283
                     /gene="nhaC"
                     /locus_tag="KPK72_002400"
     CDS             9862..11283
                     /gene="nhaC"
                     /locus_tag="KPK72_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2489608.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKISRIEDEPETCLNFDV"
     gene            11547..12011
                     /locus_tag="KPK72_002401"
     CDS             11547..12011
                     /locus_tag="KPK72_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2489609.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12245..12607
                     /locus_tag="KPK72_002402"
     CDS             12245..12607
                     /locus_tag="KPK72_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2489610.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13202..14038
                     /locus_tag="KPK72_002403"
     CDS             13202..14038
                     /locus_tag="KPK72_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489611.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14122..16236
                     /locus_tag="KPK72_002404"
     CDS             14122..16236
                     /locus_tag="KPK72_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG2489612.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFKLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSRFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            16777..17730
                     /gene="blaCDD"
                     /locus_tag="KPK72_002405"
     CDS             16777..17730
                     /gene="blaCDD"
                     /locus_tag="KPK72_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2489613.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYNVRE"
BASE COUNT         7158 a         1944 c         3294 g         5497 t
ORIGIN      
        1 atacttaaaa atatattaat ataatgtaca taaaaaatat cataaatata tgataagata
       61 aatacaatag caacatttta ttaatttttt gggtttttat atatttaggt actggggtga
      121 tatagttgaa ccaagagata ttttttaaaa taatgaatga aatattggaa aatattgatg
      181 aaggggtaca ttttgttaat aaagacggga aaactataat ctacaataat tctgtacaaa
      241 aaatggaaaa aatgaacaga cacgatattt tagccaattc attttttgat atagtcaaca
      301 aaatgaaaat aaacaggagt acactattag aagttttgga aaaaagggaa gttatcaaag
      361 acaatattca aaaatatctg gataaaaatg gaaaagagat aacagcaatt aatactacaa
      421 taccaattga tattaaaggc gagtttgtag gggcacttga gatatcaaag aatatgacaa
      481 cgatagaaaa tttactaaat agaaattata aaaaaaattt agtcaataaa gttagtagta
      541 gacaaaaaaa gggttatgaa tttgatgata ttttagggga aagtaatttg attaaagaag
      601 caatagaaaa atctaagaaa gcttgcaagt ctgatgcttc agtatttata tacggtgaaa
      661 ctggaacggg taaagagtta tttagccagt ccattcatta tggaagtaat aggagaaact
      721 atccatttat agcacaaaat tgtgcagctt tgccggaatc actattcgaa ggtatccttt
      781 ttggaactag caagggagga tttacaggag caatagatag gccaggctta tttgaacaag
      841 ctaacaaagg gacattgtta ttggatgaga taaattccat gcctattatg ttacaggcta
      901 aactattaag ggttttacaa gaaggttatg taagaagggt tggaggaact aaggatatac
      961 ctatagatgt aagaattatt gcaacgacaa atgaaagtcc aaaggtaatt ttaaacgagc
     1021 ataaaatgcg aaaagattta tattataggc ttaacataat tcatataaaa attcctcctc
     1081 ttagagatag aaaagaggac atacctctat tgtgcaaaaa atttatttca aagtataata
     1141 aaaagttaaa taaaaaagta gtaggaattt cagatgaagc tattaaaatt ttaaaaaatt
     1201 acgattggca aggtaacata agagagctag aaaacataat ttattgtact atgagtatga
     1261 tggaagatga ggtacaaata gaaacaagta tgattaatct aaacgactat tacaatctta
     1321 aagagaataa agagagtaac aattattctt taaacgattt ggaggacaaa acacttagta
     1381 agattatcag tgagatagaa gaaaagtata tatatgaatc attagaaaag gcatcataca
     1441 atataactaa agctgcatca attttaggta tgaataggca aaatttacag tataaaataa
     1501 aaaaatataa tatcaaaata ttataaataa gcaagataaa aaaacaaaat caaagtaaaa
     1561 taaaaaagca aaatagagga gcaaaattat tataaaagag caaaatattt tgctcttttt
     1621 tttatttctc ataagaaata ttatttttta tttatgaaaa tacataataa aataattata
     1681 gtttcaacgt tccaataaat atttacttta attttaaagt tggcacgatt tatgctttaa
     1741 ctaattattg agaatatata aatttttagg agggtgtata tatgagaaaa gtaagagtag
     1801 gaatatgggg atttggagct atgggaatag gtatggctaa tatgattcta aagaaagaag
     1861 gtatagaaat agtatctgtt tgtagtagaa gtacttcagg gaaaagtatg tatgatgttt
     1921 taggaattga aagaggagaa agaccagaag ttattatcaa taaaaattat gaagaagttt
     1981 ttagagaaaa aagtgtagat gttgttttgt tagcaactga ttcatttact aaaaaggctt
     2041 ttgataaaat aattttctta ttaaatagaa aaataaatgt tatatcaaca gcagaacaaa
     2101 tggcatatcc acaagctgat gatgcagatt tagctaaaaa aatggatgaa gttgcaaagg
     2161 aaaatggagt aagtatactt ggaacaggaa taaatccagg atttgtatta gatttactag
     2221 tattggcact ttcaggtact tgtgaagaag ttactagtat aaaagctaag agagtaaatg
     2281 atttatctcc atttggaaag tctgttatgg tggaacaagg tgttggagta acaagggaag
     2341 aatttataaa aggtgtagaa gataagacta tagcaggtca tgtaggtttt gtagaatcaa
     2401 taaatatgat agctgatgga ttaggttgga aattagataa gatagaacaa acaaaagagc
     2461 caataatgac tacagtagat agaaaatcta agtacggaga ggcattggct ggaaatgttg
     2521 ctggttgtag acaatgtgga tatggatatg taaatggaga agttttaatt gaaatggagc
     2581 atccacaaca gataatacca gaagcagaag gtattaaaac aggagactat gtatcaataa
     2641 aaggtatacc aaacattgat ttacaaataa atcctgaaat acctggagga gtaggtactt
     2701 atgcaatgat agtaaactct attccattaa taataaatgc tagaccaggt cttaaaacta
     2761 tgttggatat accagtacct agagcaataa tgggagatat aagaaatcag atagaagttg
     2821 aattggaaga agaatcaaag gctaattagt aagataacag ataactaatt agtaagatag
     2881 caactagcta attagtaaga taacagttag ctaattggta aaataatgtt taatttaaaa
     2941 taacagtaaa ttaattagta aggcaatagt caattaataa ggggatgaaa aaatggatgc
     3001 taaaattaat gattgggtta ttatacacaa tatagtattg actccagaag aaagagctcc
     3061 acaagttcca gaggatacta agaaagtatc cttagaaatg tgggttaaag gttttataca
     3121 aagtgatgct tctataggcg acttagtaga agttaaaaca attacaggca gacttgtaaa
     3181 aggtaattta cttaaaataa atccatatta tactcatgat tatggtaaat gtataccaga
     3241 gttactccaa ataggaatcc aagctaaaga aatattattt ggaggtgtat ataatgaata
     3301 attcaacatc aatgaaagat atgagttatc aagctgttat gggaagaaat aacgaaataa
     3361 tgaaaaatgc aatcggattg gattattctt cttttgaaca agagggaata gggtttgatt
     3421 atgagaaaat gatgagtgaa acaggatata cattacaaga tattgaagca attcaatcac
     3481 agtatgctgt tggaaatact cctctaatag agcttaaaaa tttgactaaa ttagctagaa
     3541 aatgtgctaa agaaggtaaa ggagctagaa tatttgtaaa agatgaagca atgaatgcat
     3601 ctggtagttt taaagcaaga agagcagcta ctgcagtata tcatgccaaa caaatgggat
     3661 ataaaggtgt aatagcagct actagtggta actatggtgc agctgtagca tcacaagcgg
     3721 caatgcaggg attaaaatgt ataatagttc aagaatgtta tgattctaat ggtgtaggtc
     3781 aacctgaaat catagaaaaa gcgcgaaaat gtgaggcata tggagcagaa gttgttcaat
     3841 taactgttgg accagagtta ttttatacgt tcttagtttt attagaagaa acaggttatt
     3901 ttaacgcatc attatattca ccatttggaa ttgcaggtgt agaaacttta ggttatgagt
     3961 tagctatcca atttagagaa aagtataaaa aagaccctga tatagttgtt tgtacaaatg
     4021 caggtggtgg taatcttact ggaactgcaa gaggattgat aaaagctggt tctattaata
     4081 ctaaagtagt aggagcaagt gtagatttaa aaggattaca catggctagt gataatcaat
     4141 ttaataaaaa atcatttaca acaggacata caggttttgg gataccatac tgtacatggc
     4201 ctgataggtc agatgtacct aggtctgcag ctagaccact tagatatatg gatagatatg
     4261 tattagtaaa acaaggagaa gttttttata caactgaatt attggcacag ctagaaggta
     4321 tagaaagagg tccagcagga aatacatctt tagcagcagc attctcttta gctcaagaat
     4381 tagatgagga taaagttata gtggtacaag aaacagaata tacgggtgca ggtaaacata
     4441 tatgtccaca acttaccttt gcaagagaaa atggaataga tataaaattt ggaaatccaa
     4501 gagaagaaat tgcaggagta aacttaatat taccagaaag accagaatta ttgaaatgtg
     4561 ttgatattga tatgaataaa ataagaaagt catttataaa aaattgtata tctaataatc
     4621 acattgataa tattgataag ctaagtaatc gagatataga atttttaatg gaagaagtaa
     4681 aaagttcaag agattttgtt gttgatgtat taaataattt atagtaatta ttgtttttta
     4741 atttaatata aaataaaaat tcagaaaatt cgattaaatc aatacgaatg gaggaatgtt
     4801 catgaaaaaa agagaggatg attttgaagt tagaagaaaa catcttcaag aactgagtga
     4861 tgatgagtta aaagaaaggt tttggagttt agctacccag atagtagaac caatgataga
     4921 acttggtaaa aagaatacaa caccatcaat agaaaggtct gtcttgttga gaatgggatt
     4981 ttcttctctt gaagtaaaac ctattttaga aggggttatg gaaagaggtc ttataggtaa
     5041 aggagcaggg catgtagttt ataaattggc taaatctaaa aatataacgg ttagagaagc
     5101 aggtttgtta ttagttaagg gtgaatattg ggatgaagtt acatgtttat ttagagaagg
     5161 ggtagaatca tgttaaagga aaataaaaaa ttggatatag actatatatt aaaagatttg
     5221 gataaatata aaccaaaacg aagaggttgg gtttggagag aacacttgga aaatcttgaa
     5281 atgggacctt ttaaatataa agactgtaca aaacctttaa agaagagtgt agggctacca
     5341 tcatctaagt atttcaataa tatagaccct caaccaagtc cagttattac tactgaaata
     5401 gcttctggta gatttgagga tgatataaga agaatgagaa tggcagctca tcatggagct
     5461 gaccatctaa tggttatcag aactgctgga caatcacatt ttgatgggct tatagaagga
     5521 actccacaag gtataggtgg agttccaata acaagaaaac aagtaagagc tcaaaggaag
     5581 gctcttgatt ttatagaaga agaagttggt agacctatta attaccattc atatgtaagt
     5641 ggtgttgctg gtcctgaagt agcagtaatg tttgctgagg aaggagtcaa tggagcacat
     5701 caagacccac aatataatgt tctgtatagg aatataaaca tggtaaggtc ttttgtagat
     5761 gcttgtgagg caaagaaaat aatggctttt gccaatatag ctcaaattga tggagcacat
     5821 aatgctaatg ctactgctag agaagcatgg aaggtaatgc ctgaacttat ggttcaacac
     5881 gctttaaact ctatattctc agaaaaaata ggtatagata aaagtaatat atgcttatca
     5941 acagttccac caacagcacc tccagcacca tgtttaaaaa tagacttacc ttacgctgtt
     6001 gcacttagag agttatttag tgattataag atgagagctc aaatgaatac taaatatatg
     6061 gaatcatcaa ctagagaagc aacagttact catgtattaa acttattaac atcagtatta
     6121 actagagctg acatacaatc aactataaca cctgatgaag gtagaaatgt accttggcat
     6181 atatacaata tagaagcatg tgatacagca aagcaagctt tagttggtat ggatgggctt
     6241 atggatatga tagaacttaa agatgtcggt gaattaagag acaaagcaag agaacttaaa
     6301 gaaagagctg tattatatat ggaagaaata tctgaagttg gtggatattt tgaatctgtg
     6361 gaacaaggat tttttgttga ttctggaaat tatccagaaa gaaatggtga tggaatatct
     6421 agaaaaataa aaggtggagt tggtgaaggt actgtttatg aaagagaaga agattattta
     6481 gcacctgtaa ctgctcactt tggatacaat aatgtagctc aatatgatga aaatgctata
     6541 gataatccat caatgttaat agatggatgt acatttgaaa atccagacaa aataatatat
     6601 attgatgagc ttgacgattt tgataaagta gaaaatagac taaaagaatc atatgagtac
     6661 agaaatggaa ctaaaattaa gcctgaaatg gaatggtgtg cagatggaat tgtaatgata
     6721 acaatgatgc ttcctactga taaaagaaca gcagagtttg cagctttgga atttgtcaaa
     6781 aaaatgaact tacaagaaat tgaggtcata agtagagaag ttatgcatga atctgaaggg
     6841 actagaatag aagtgaaagg aagagttccg tttgatatag atttaaacaa tttagtaata
     6901 ccagaagaac ctaaggtact aactgatgaa gaaataagag cagatataga agaaaaacct
     6961 atgaaaatag tttcagctac ggttggagaa gatgaacatt cagttggttt aagagaaata
     7021 atagatataa aacatggagg tattgaaaaa tatggcatag aatgccacta tctagggact
     7081 tctgtacctg tagaaaaatt ggtagatgca gcgattgaac tgaatgcaga tgcaatactt
     7141 gcatctacaa taataagtca tgatgatatt cactataaaa acatgaaaaa attacacgat
     7201 tattgtgtag aaaaaggtat aagagataaa gtaatgattg cgtgtggagg aacacaagta
     7261 acacctgaaa tagctgttga acaaggcata gatgctggat ttggaagaaa cagcaaaggc
     7321 atacatgtag ccactttctt agttgaaaaa agaagagaaa tgagtaataa ataatggaaa
     7381 atagcaaggt ttctttaaaa atagatgtgt tggtagctga aattggaagt actactacag
     7441 tggtaaatgc tttccacgat attaatacta acaatcctat atttttgggt caaggtcaag
     7501 ctccaacaac agtgtttgaa ggtggcgatg taagaaatgg cttatcaggt gctattaaag
     7561 atttagctaa taaattatct gtagatgata tagagtataa tgatatgttt gcaacttcta
     7621 gtgcagctgg tggattaaaa atgactgttc atggattagt ctatgatatg acagtaaaag
     7681 cagctaaaga agcagcttta ggggctggag ctgttattcg tcaaataaca tcaggtagaa
     7741 ttaaaagaac tgatttaaat aaaataaaag aaataaaccc aaacataatt ttaatagctg
     7801 gtggagtcga ttatggagaa agagatacag ctatttataa tgcagaaatg atagcttcta
     7861 tgaatctagg cataccagtt atatatgctg gtaatgttga aaatcaagag gaaataaggt
     7921 taatatttga agatactaat tataagcttt atataactga aaatgtatac cctaaaatag
     7981 atttattgaa tatagaacct acaagaagaa tcattcaaag tgtatttgaa gaacacataa
     8041 ctacagcacc aggaatgaaa tacattaaag aaatggttaa tgaaaatatt actccaaccc
     8101 caggagctgt aatggaagct tctaaacttt tatataaaaa tattggagat ttattaactc
     8161 tggatgtagg tggagcaact actgatgtac attctgttac agatggaagt gactatatta
     8221 ataaaatttt ggttaatcct gaacctactg caaagagaag tgttgagggg gatttaggtg
     8281 tatatgtaaa tatgaagaat atagtagaag ttataggtaa agaaaactta caaagtgaac
     8341 tatctataga tattgatgca gtaatagaga attatcctcc aataccaaaa tcaaaagaag
     8401 aaatattatt tgttgaaaga ttgacaaaag aagctgttgt taaagctatg ctaagacatt
     8461 caggaaaaat aagaaatatc tatggaacaa ctggaaaagt caaaattgca gaagggaaag
     8521 atttaacaga agtaagatat atagttggta cagggggagc acttactagg ctccctagta
     8581 gaattaaaat attagataat atgctcaaat acaataaaaa taatgaatta ctatttccaa
     8641 aggaaaaaac aaagatatta atagataatg actatattat ggcatctatg ggtgttttat
     8701 ctaaaaaata tgaagaagca tctcttaagc tcctgttaaa aagtttgaat tttgaggagg
     8761 aaagattatg tatcctaggt tagaaataga tatagaaaag ttaaagcata atgcaaaatt
     8821 aatttcgcag atgtgtcatg aaagaaatat taagatatct tttgttacta aatcattttg
     8881 tgcacaaaaa gaaatagtag aagaaatatg tagtgaagga atagaccata tagcagattc
     8941 aagaatacaa aacttaaaaa atttacaaga tataaatcta cctaagatat tgattagaat
     9001 cccaatgtta agtgaattag aagaagttat caattattgc gatattagtt ttaactcaga
     9061 attaggaaca ataaagaaat taaatgaatt atgtgaaagt aaaaatatta ttcataagat
     9121 agttcttatg tttgacttag gcgatttacg agaaggatac ttttatgaag aagacttttt
     9181 tagtaatgta agagagatag taagattaaa aaatattgaa ataattggta tagctacaaa
     9241 cttaacctgt tatggagcta ttataccatc tagggaaaat ataggcagac tggtaagtat
     9301 agcaaaaaga atggaagaaa aatttggaat aaatctagaa atagtatctg gaggtaattc
     9361 aagttcgatt catctgttga taaataataa tatgccagaa ggtattacta atttgagaat
     9421 aggcgagtcc attctacttg gtagagaaac ggcatacggt gaaaatataa atggaacata
     9481 tcaagatgca tttaaattaa tatgccaagt agttgaatgt aaagaaaaac catcagttcc
     9541 tattggagaa ataggagttg atgcctttag aaataaacct gtttatgaag ataagggaat
     9601 cttaaaaagg gcaatcatag ctataggtaa acaagatata aatattgact ctttaattcc
     9661 aatagacact gatataaaaa tattaggggc aagttcagac cacatgatac tagatgtaag
     9721 taacacaaaa tatgattata aattaggtga caatttagaa tttttattaa cttatggggg
     9781 tatcatgtca tctagtacaa gcaaatatgt ttcaaaaaaa attatagtgt agtgtttaaa
     9841 taattaggag gaaactattt tatgaaagag cgagaatgta gaaaagcaag tctgtttgat
     9901 gcaatgatac caattacttg tttgatatta tttttaagtc taggagtatt agtgtatggt
     9961 tcatcacctc atgtaccact aataggagca gcagcagtag caggacttgt ggctgtatat
    10021 agacttggat ttaaatggaa agagttagaa ttaagtatgt ttaatagtat aaagatggca
    10081 atgcaagcaa tcttaatcat aattataata ggcgtactta taggaacttg gatagtaagt
    10141 ggagtagttc catgtatgat ttactgggga ctaaaaattt tatcaccaaa tattttttta
    10201 gtagcatcaa cattagtctg tgcaatagta tctcttgcga caggttcatc ttggagcaca
    10261 atgggtacag taggagtagc tcttttagga attggtcaaa gtttaggtat gccaattgga
    10321 ttaatcgtag gttctataat atctggagct tactttggag ataaactatc acctttatct
    10381 gaaacgacta acttagctcc agcaatggca ggaacagatt tgtttacaca tataaaatat
    10441 atgctatatt cgactatacc atcattatta atttgtttag taatttatgg tgtaattgga
    10501 atgaagtatt caggtcaagc actagatata aaacaaatag aattaattag gtctacatta
    10561 gattctacat ttaatacttt atcaccagta ttattattag ctccagcaat agtaattgga
    10621 ttagttgtat ttaaagttcc cgctatacca gggcttatag taggtgctgt attgggagta
    10681 ttgtttgcag tagtttttca aggagaatct atgaatacta tattagatgc agcacaaaat
    10741 ggatatgttt cgtcaacaca aatacaggag gtagatgctt tattatctaa aggtgggatt
    10801 atgaatatga tgtccactgt atccttaaca atatgcgctt tatctcttgg aggtatttta
    10861 gaaaaaacag gtatgcttga agtagtagca tcttctttac taagattagc aaagggtgta
    10921 tttggaacag tattatgtac tatggtaact tgtacaatta ccaatataat agcaggtgaa
    10981 caatatctat ctatagttat accaggaaga atgtataata aggaatataa aaagagaggt
    11041 atacatccta agatgttgtc aagagcttta gaagatagtg gaactttaac ttctccatta
    11101 gtaccatgga atacatgcgg ggcgtatata acagcaacat taggtgtatc tgcactaaca
    11161 tatggtccat acgctttatt aaatattata aatccaatag tatctttagt attaattgca
    11221 tgtaagttta aaatatcaag aatagaagat gaacctgaaa cttgtttaaa ttttgatgtt
    11281 tgatatttta attgtttgta taaatttttt atagaaaaag atttctttta gacttgccta
    11341 aaaattaggc aagtctttta agtatgttct gattataaga gcactgtgtg acaagaatga
    11401 tgagattatt tttgctatac tttcgatata agaaatggaa ggaggttatg ttttatattc
    11461 atatgaaata attagttaca cagtgaattt tcgtataatt tggtataaaa agattagaaa
    11521 tgaaaataaa gtaatggagg aataaaatga ctagaatatc caatataaca atagctgaac
    11581 aatctgagta ctgttttttg gcaatacgaa aaacaattga ctttatggta gaattttcag
    11641 agttttcaaa acaaagtttt gaaaaaattt ctaaatatct ggaggagaga ggaattttat
    11701 cttcaggagc accgattgta tgttttcata acatggattt agtaaaattg gatgttgaag
    11761 tagggtttcc tgtagccact tatatagatg ggaaaaatga gattctacaa aagattgttc
    11821 ctgcacagaa aataataact gcaattgatt taggacctta tgaattacaa gaccctactt
    11881 tagaagactt attttcatgg gcaaatagca atggatataa acttcatggc gatatatatt
    11941 accagtattt aaatgatatc aatcgtccag ctagtgaata tcttacgaaa atgatgttac
    12001 ccattatata gaaagttaaa gattatcgtt tgttagttga taattagaag aacatttact
    12061 gctactacat aaattttatc ctaaataata tcaaggtgat ttaatttatg tattctaatg
    12121 taaccccaat atattgttaa ggtctactta agatgctcaa aaggctatgc ttaatttatt
    12181 gttatataat ttgtagagtg gtataattta gaggtaatat tatatagata tataggggga
    12241 aaatatgaaa atatatgata tattaacacg tttttatatt caagatatag aaaaagccat
    12301 acctttttat gagaatcttt taaaagaaaa atgttctttg cgtttttcct ataaagaagt
    12361 tgggttggaa ttagcgcaaa taggaaatgt tttactatta tctggttctg atgatgctct
    12421 taaaccattt atagaaacaa aatctacttt tatggttgat tctgttgatg aatggagaag
    12481 ttatctattg gataatggag cagttgtagt aagagataag aaaaaagttc ctacaggata
    12541 taatatgaca ttgagacatc cagatggtac aattatagaa tatgttcaac atacaaagca
    12601 ggattagtta aaaacagatt ttactactga gcaactaatt agaatctatt cttagacgta
    12661 caatttatta tttttggtat taacagatta taagtcaaat tataatattt caaaatagaa
    12721 tagaaattta tttacataaa agagagtgtt taaaaatgaa ctttttagtt catgaagcac
    12781 tcttttttgt atgaaatcaa atacatcaat atataaaata aactgatgaa gagcttaaat
    12841 taaaataaaa agattcataa caggacccca ttaaacttta agatagggta agtttgtaat
    12901 catattaaat aattgtaaaa agctgttttt agtcgtataa tataaatatt tatcatttaa
    12961 ttaaggaatc taaaaggaat ctaataagaa gaaaataata aataactttt tagatttact
    13021 aaatgaaaag ttattgaaaa ttaaaggatt agagtcttta atcagaaagt aaaaagtaat
    13081 atattaaatt tatgaattga aaacttaaac aacaaatttg tttataaaat agcaagattc
    13141 attaaatata actaagtaat aattgatata atgttgcatg gctaaaagga gggaagctgc
    13201 aatgtcttac aaagtaatta atgtcataac tgctattgac tatatagagg agcatttatc
    13261 agaaaagctg gatttagata tagttgctaa tgctgttcat tactcaaaat atcacctgca
    13321 tagaactttc acaacttctg ttggcctaac aatgcatgat tacataaagc ggagaaagtt
    13381 gacagaagca gcaaaattat tggtattttc aaagaaacca attattgaaa ttgcattgat
    13441 agctggatat gaaagtcaac aggcattcac ttctatattt aaagctatgt ataaaaaatc
    13501 tccaaataaa tataggaaag aacaagaatt ttatcctttg cagctaagat ttgttttgaa
    13561 aaaagataac ctattttcaa agaatgtact agagttggaa aaagaaatta aacttgcaag
    13621 tatgtctgac ataccattat ggatgaattt agtgagatta gtcatagatg gatttccaaa
    13681 tttacaagag gaagaatata tatatcaatt agaacagtat attttagaag aaagagcttt
    13741 aatattaaaa cttaataatg tcgctattgc aaatatggtg tttaatagag aaactagaag
    13801 tattgatttt tttggaatac atccacagta tcgtaattca gacattgcac aagtgttttt
    13861 gaagaaagtt attgaagatt tcttgataga tacagatatt tcaattacta cctttcgaga
    13921 gggtgacaaa gctgatacag gacatagaga tatgataaag aagttgggct ttgcagaagc
    13981 agagttgctt gtggaatttg gatatcctac tcaaaaattt attcttcctt taagttaaat
    14041 agcaagaatg attaaaaact ccaaaacaca attaagtata atgattcagt gtaagtactt
    14101 aattaacaaa ggaggattta aatgtctgaa aataaacaag tagtaaattg cgatattatt
    14161 gcatctaatt ctaaacataa tatgtgtgaa tatgatgcag agttattaac tttttttcca
    14221 gatgagattg cacatctaat agagataaat aacaagctag acaatgcttt taagaaagct
    14281 gaaaatcttg ttgacaagct tgataaggat tatatggatg ctaaaatgta tatggtaaaa
    14341 aatcgtggtg aaatagaccc acatgaaatg tttcaaaatg aacaaggact aaaacaaata
    14401 gataactatg gggcttttat ggtaaaagtt cgagataaga ttaataaaat caaagattct
    14461 ccatactttg ctagaataga ttttaagctg aaagatatgg atgatgaatc taagtattat
    14521 attggtagat ttgcatttga ttatgaagat gagcttataa tacttgactg gcgttctcca
    14581 attgccagta tgttttatga ttatgaaatc ggaaaagctg gatatgatgc tccaatagga
    14641 tgggtggatg gagaaataac aagaaaacga caatttaaaa ttaaaaatgg aaaattagaa
    14701 tatgcactag aaagttccat taatatacaa gatgatattc ttcaaaaaga acttagtcac
    14761 acttcagatg aaaaaatgaa gtcaatcatt tcaacaatac aaaaggaaca aaatcaaatt
    14821 attagaaatg ataaagctga tacactgatt attcaagggg tagctggttc tgggaaaaca
    14881 tctatagcat tacacagaat tgcttttctt ctttatcgat ttaaagataa aatatcagca
    14941 aataatgtaa ttatcctatc acctaataaa gtctttggag actatatttc caatgtcctt
    15001 cctgagcttg gagaagaacc attatgtgaa ttaagttttg aaaatatagc agaggtacaa
    15061 ttagatagag ttattaattt tgaaagtgag aaagacccat tagaaataaa tgatgctaaa
    15121 tggtctgaaa gagtgcgttt taaatctaca cttgattttg taaaacttat tgatgattat
    15181 ataaaacaaa tgccaaataa gatttttatt ccaaaagact acacttttgg aagttttaca
    15241 gctaaaagtg actggataca gagtagattt gaggcatata acagatatcc agttaagaag
    15301 agattagaga aggtagctga agacattcat tataaatttg aatcagataa tattatggaa
    15361 gaagatttac caaaggtaaa aagtatactt aaaagtttga atggaatgtt gacaataaaa
    15421 aatactctta cactatataa ggatttcttt aaacaaatga atatatcaaa catgtttgtg
    15481 atggcagaaa agaaaacact tgaatggtca gatgtatacc catttatata tattcatgct
    15541 gcatatgaag gtatacaaga agataagatt ataaggcata ttgttattga tgaaatgcag
    15601 gactatacac ctattcaata tgctgtaata aacttacttt ttaagtgtaa aaagactatt
    15661 cttggagatt ttggacaatt ggttaatcct aatcatactc atacactaga tgatatgaaa
    15721 cagctatata atgatgggga actagttatg ttgaataaga gttaccgttc tacttttgag
    15781 attattaatt tcgccaaaaa gattcaagac atatcttcat tagagcctat tgaacgacat
    15841 ggtgaagaac cagctttatt aaaatgtaat aacgagcaag atgaaataaa taagataaaa
    15901 atagaaattg aagaatttaa aaagagtgat aatgcgactc ttggtattat attaaagaca
    15961 gataatgacg ctaaagctgt atatgatgcg ttaagagaag aatatagtgt tcacttaata
    16021 tcttctgaaa gttctagatt tacaaaagga gtatccataa cttctattaa aatgtcaaaa
    16081 ggtttagagt ttgatgaagt tattgtacct tcagtaaata ataaaacata ttatagtgat
    16141 tatgaccgca gtttgttata tattgcctgt acacgagcta tgcataaact taagttgaca
    16201 tatactggtg aattgactca acttatagat atgtagatta tatacttatt atgtaagtgt
    16261 ttacttatat aaatataacc tgtcaataaa attcattaat aaatcaataa aaacaatatt
    16321 tgtaaaagta aaagctataa attatatatt aaattttata atttatagct tttataatac
    16381 tgcttatctt aatcctccca atagcaaaat aattttaaac atgccatatt ttataaatgt
    16441 aataacaatt atacaattaa aataactttc ccaaactaac ttgacattta aaacttacta
    16501 ttgtaatatt taaatattac aatagtaagt tttaaatgtg gaatcaatta ttatatcaca
    16561 aattaaaagg aggacaggat gttattaata gttacctaat tatcttcgca ataggtttaa
    16621 tcaacataat tgtactaggt gaagaaaata taagagatac agctattcat ttagtcttgt
    16681 ttcttataag tagaacctag attggagtat attacaaata atgtctacta ttttaatagt
    16741 aggttataaa tttaaatatg ggagatgata taaattatga aaagaaaaaa gaattttatt
    16801 tggatagcta tcttattggt tggtgtagtt atggtaatgt attatgtagg gaaaaaacat
    16861 aacgatataa atcaaaaaac tgataagaat tatataaaat ctgaattaaa taagtctgaa
    16921 ttaaataaat ccaaaattga aaacaataat aaaaacaaaa aagaaaaaat taatatggtt
    16981 gactatagtg attgttttga aggtattagt ggaggagcta ttttttataa tactaaaaat
    17041 aaagaatata atatatataa caaagaatta attgaaacaa gaaggtcacc atgttctact
    17101 tttaaaatag tttcaacttt aattggttta gaaaaagggg taataaactc aaaagaatca
    17161 gttatgggat atgatggtac agagtatcca aataaaaatt ggaataaaaa tcttagttta
    17221 gaagaggcat ttaaggagtc ttgtgtttgg tactacaaaa aattgataga taaagttgat
    17281 gctaaatctg tccaaaatat tttagatgat ttaaagtatg ggaattgcga tattagcgaa
    17341 tgggaaggag atttaaaaaa tggaaaggga catttaaatg gattttggtt agaatcttca
    17401 ttacaaatat ctccaaaaga acaagtgcaa acaatggcaa aaatatttga gggagataca
    17461 aactttaaaa aagagcatat taatatttta agagatatta tgaaaataga tgtaaatgat
    17521 aaaaacataa atgtatatgg aaaaactgga acaggatttg atgaaaaaaa taaacgtgtg
    17581 gatgcatggt ttgttggtat gctagagcgt gaaggtgaca cctattattt tgctattaaa
    17641 tcagatgatt ccaataaaga aattacaggg ccaaaggtaa aggaaattgc aattaatata
    17701 attaagaaat attataatgt aagggaataa acttaatttt atttgtaaat attatttacc
    17761 tatactctgt aatatttatt aaataatttt actaaattga atctatatag agcaaaactc
    17821 ctttagtaca taaaatactt gctattgatt attaaattga atacctatag atttaaagtt
    17881 aatacctttt aaa
//
LOCUS       DAEMHZ010000078        17816 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000078 DAEMHZ010000000
VERSION     DAEMHZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.078"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..620
                     /locus_tag="KPK72_002406"
     CDS             75..620
                     /locus_tag="KPK72_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2489614.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            633..1646
                     /locus_tag="KPK72_002407"
     CDS             633..1646
                     /locus_tag="KPK72_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2489615.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     VLR"
     gene            1868..3841
                     /locus_tag="KPK72_002408"
     CDS             1868..3841
                     /locus_tag="KPK72_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2489616.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTILLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3910..4269
                     /locus_tag="KPK72_002409"
     CDS             3910..4269
                     /locus_tag="KPK72_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489617.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4304..4660
                     /locus_tag="KPK72_002410"
     CDS             4304..4660
                     /locus_tag="KPK72_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2489618.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4697..5989
                     /locus_tag="KPK72_002411"
     CDS             4697..5989
                     /locus_tag="KPK72_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2489619.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDIAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6425..7063
                     /locus_tag="KPK72_002412"
     CDS             6425..7063
                     /locus_tag="KPK72_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489620.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7070..7912
                     /locus_tag="KPK72_002413"
     CDS             7070..7912
                     /locus_tag="KPK72_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG2489621.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            7973..10600
                     /locus_tag="KPK72_002414"
     CDS             7973..10600
                     /locus_tag="KPK72_002414"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2489622.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            10814..11059
                     /locus_tag="KPK72_002415"
     CDS             10814..11059
                     /locus_tag="KPK72_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2489623.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11348..12646)
                     /locus_tag="KPK72_002416"
     CDS             complement(11348..12646)
                     /locus_tag="KPK72_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG2489624.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEIDRYSEGSNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDEYKADLPDRPSLTRPVAPTKP
                     EQGNLTDDEYEVLMDKYKKDYEQYKIDVTEYNNERKTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(12680..15814)
                     /locus_tag="KPK72_002417"
     CDS             complement(12680..15814)
                     /locus_tag="KPK72_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG2489625.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(15818..17041)
                     /locus_tag="KPK72_002418"
     CDS             complement(15818..17041)
                     /locus_tag="KPK72_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2489626.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LIYDGTKIFPVEKEK"
     gene            17293..17778
                     /locus_tag="KPK72_002419"
     CDS             17293..17778
                     /locus_tag="KPK72_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2489627.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
BASE COUNT         6482 a         2274 c         3016 g         6044 t
ORIGIN      
        1 agatgtaatt tgtaaaattt atggtaagca gtaaaattaa aaatttaaag atattatagg
       61 gaggttataa ttttatggaa aacgttatta caggtctaag taaaggtgca gagtggttta
      121 tcggtttgtt tcagaaaggg ggagaacaat ttataggtct tgtttcaggt actcttccaa
      181 cgctaattgt attaatggta gctattaact ctttgattaa gataattggt gaggaaagag
      241 ttaataattg ggcttctaag ctaggaaaga actcttttac aagatatatt ctacttccat
      301 tagtatcagt atttttcttg ggaaatccta tgtgctatac atttggtaga tttttaaaag
      361 aagagcacaa agcaggattt tatgatgcag cagtttcatt tgtacatcca attacaggtc
      421 tttttccaca tgcaaatgca ggagaacttt ttgtatggtt gggaatatct gcaggtttga
      481 ctactttagg taaagaaact actacactag cattgtggta ttttatagtt ggtctaattg
      541 taatttttat aagaggtata gtaacagaaa aaatgtatgc ttttctaaca agaaaaaata
      601 gagcaacaaa taaggcttag gaggtagata aaatggagga gaataaaata ttaaaaatag
      661 aaaagggaac ttcaggctgg ggaggtcctt tgtatataaa aaaagaaggg aatagaaata
      721 agatactatc aatgactgct ggcggtatcc atgaagtaac attaaaaata aaagaactac
      781 tgggttgtga aattgtagat ggatttaaaa ctggagtttc tgatgaagaa gtagcagtag
      841 ttattattga ttgtggtgga actgctagat gtggagttta tccaaagaaa aaaataccta
      901 caataaatgt aaatccagtt ggaaaaacag gtcctttagc aaaatttata acagaagaat
      961 attatgtatc agatgttaat cctaactgta taagtgttgt agatggggaa gatatgccac
     1021 aaaaatctca agaaaataaa agcgaaaata agagttctat tagaaagcct gataattatg
     1081 atgaggttaa gtcaaaagca caaggtgaat atgctaaaaa gaacataata ctttctattg
     1141 gtcaaggggc agggcaagta gtatcaaaat tttatgatgc aggtcgagat actatccaaa
     1201 tggtaatgaa taacgtaata ccatttatgg catttgtaag tatgcttatg ggaattattt
     1261 tagcatctgg actgggagac tggattgcaa gagttatatc tccacttgca ggtaatattg
     1321 gtggattatt gattatatca gtaatttgca cacttccatt cttatcacca atactaggac
     1381 ctggtgcagt tatagctcaa gtagtaggga ctcttgtagg tacacaaatt ggtcttggtg
     1441 caataccagc atatttagca cttccagctt tatttgcaat aaatgggcaa gctggatgtg
     1501 attttgtacc agtaggatta agtttgggag aagcagaacc agaaactgta gagtatggtg
     1561 ttccagcttt attttactct cgtttaataa ctggtccaat agctgttatt attgcatatg
     1621 gagttgcagt atttgtattg agataatatt taatgtcaat ataaggcttg gaattaatta
     1681 ataaaattgt aaatataaat ttaagctagt caagattttt tgttaatgaa agactagctt
     1741 ttatactatt aattagctgt aattatcgag ttagattaat actaaatttt cctttattaa
     1801 agtgaaattt tatgtattaa tttggtttca aatttgtact agaatgaaat caaggaggag
     1861 atacaaaatg agtgtaactg agaaaattcc ttctattgac cgcaagatat ttaagatatt
     1921 gattatgtgt agtaaaaaag agtttgtgag tattaactca atagcaaatg aattgaatgt
     1981 cacaactaga agtgtaagga cgtatataaa acaattgaat aaagatttag gtaatgatat
     2041 agcagtaatc aaatatataa agggtcaagg ctataaacta gaaataaaag atgagcagat
     2101 actcaataag attattgata tgaatagaaa aaatgtattt tcattaaatt caaaagaaga
     2161 tagagtagag tttatactaa attacttgat agaactagat ggatttatta cacttgatag
     2221 tttagctgat gagatgtgtg taggaagaac tactttagta aatgatttcc agtatgtaga
     2281 aaaagttttg gcttcatata atttaaactt gattaaaaaa cagaatacag gtatgaaatt
     2341 aaatggaaat gaacttgata ttagactgtt tatattaaat cagttgtata aaaactcaag
     2401 gaaagatttc agtaattcaa agtattttaa aggaatcaaa aaagaagaaa tcataaattt
     2461 agaagaaaaa ctattgactt tatttaaaaa aaataatttt tatgttacag atgaaatgtt
     2521 aagagaagtt attaactaca tagtagtttt agtctataga gtaaaagaag aaaagaaagt
     2581 aaaagattat gatgtaaagt ttgatttact taaatcatat gatgaatact ttattgctag
     2641 ggaaataaaa catataatat caggaatgtt tgaatgtact ttaaatgatg aagaaattat
     2701 ttatttaact atactgttag ttagtggaaa tgcaccagca tcagaatgtg ctttaaatag
     2761 tagtagaatt agtaaaaaca ttgatgagtt gatggaaaat atatttaatc aaatatatgt
     2821 cgatatgggt atttttataa atgaggatga attaagagta ggtctaggat atcatcttag
     2881 ttttacttta aacaggctat tatttaatat aaagttaaaa aatgttttgt tagaagagat
     2941 taagcaaaat tatatacttc catttaaatt agcacaaata gcaggaaaag taatagagaa
     3001 aaagtataac ttagaagttt cagaagatga gattggatat atagcaatcc atttcagtgg
     3061 atatttagaa agaaatagca gtagattcta ttcaataaaa aaaatagcaa ttatatgtag
     3121 tacaggtcta ggaacagcaa aactattgaa aataagagta gaaaaactga taggaaataa
     3181 tcctaaaatt gatactcttt caagttttaa tttgagaaat attaacttag atgaatacga
     3241 tatagttttt acaactattg atttagacac atcaaatata aatacaatag tattaaaaat
     3301 aaatacaata tttgatgaaa acaaactaag agaacaatta aaaacagtat tgtgcctaag
     3361 agaagggaat attgacacta ctaattctac aaatttacta ataaataatt tgttagatga
     3421 agataaattt atgatattaa atgaaaagac tatacttgct tcattagaaa aaatgatgga
     3481 ccatcttatg gatttgggat gcattgatga taagtttaga aaaaatatag tcaatagaga
     3541 agaaaaatct cccacagtat ttgataaagg tttattattt cctcattctg taaatgaaaa
     3601 atcagataag tttttaatgg cagttggaat tttagaagaa ccaatagaat atgcaaatag
     3661 aagtataaaa ataattctta tgacaatgtt tccatgtgag aataagatag actcagactt
     3721 attagtaaaa atttatgaag aagttttaaa gataggccaa gatgtaaaat taacgaataa
     3781 gataagtaaa tgtagaactt ttttagaatt taaaaaggtg ttacttaaaa accttatata
     3841 actattgtac agcatttata gttgttatac agatttaaaa ataatgtgat ttttggaagg
     3901 tgtataaata tgaaattatt agtaatttta tttgttgctt atattttaaa tactatattt
     3961 ataatgaagc agaataaatt gtattttaaa actttaaata gagctaaaaa aaagggtgat
     4021 atagtgtcta caggcaaaaa gaaaagctat ttttctaaag gaagtatagc cattatatct
     4081 tcagattcag atgggtttat aaagtttgga gaaattttaa aagggcgtac agtaatggct
     4141 aaatttaaag agattgaaga aatagaagga ttagatatat atagtgcaga gcaaaaattt
     4201 aaagatgaag agagtattgt acaagctata aaatttataa aagaaaaaat aaatgtttct
     4261 tttaattaac tcaatataaa tataaattat caggaggaat gtcatgaaat atgaagttaa
     4321 gataagtggg ataggcagtt tagttaaaga gttaatggat gaaagcaatt gtcttattat
     4381 atatgatgaa accattaatg atgacgactt aaaggatata tctgtaattc attcaatagc
     4441 aacattaaaa agtgatgttg aaataggaga tacacttact atagggaata gagattattg
     4501 tatagtttca gttggtgata ttgctcagaa gactcttaga gaaattgggc attgtacaat
     4561 caaatttgat ggaaaatgtg aagtaaattt accaggagaa atacatgtgg aaataggaaa
     4621 tccagatata actattggag atttaataac tatttcttaa tttgaaaata aaattattat
     4681 aacaggagga attggtatgt tagatttaaa cagaaaactt gcaaaattgg aagaggaagg
     4741 taaaaaaata aaagtagctc ttataggagc tgggcatatg ggaaatggaa tggtaagcca
     4801 aatggcaaat atgaaaggta ttgaagcttc tattgttgta gatataaatt tggagttggc
     4861 tcataaagct tttactgatg caggaataga tgaagaaatt atagataatg taacaaatga
     4921 aaaggatgca gaaataaaat tacaagaagg aaaagtattg acatgtaatg attttcttgt
     4981 ggcttgtaaa actaagtcaa tagatgtagt tatagatgca actgggggta ttgcaattgg
     5041 tgcagagata gcattaaact ctattttaaa taaaaaacat atagttatgt taaatgctga
     5101 aactgattgt gttgttggac caatacttaa aaaattagca gatgatgctg gagtaatatt
     5161 tacaggttct gcaggagatg agccaggtgc agtaatggaa ttatttgatt ttgcagatgc
     5221 aatggggttt gaagttagag tgataggtaa aggtaaaaat aataaattag atttagattg
     5281 taatcctgat acagttagag aagaagcaga aagaaaagga gcttcacctc atatgatagc
     5341 ttcttttaaa gaaggaacta aaacaatggt agaaatggct ttaatgtgta atgcaactgg
     5401 atttgtgcct gatgtaagag gtggtcatgg tattgaagct acagttaatg aagtacctaa
     5461 gaaatatgca ttaaaaagcg aaggtggagt attagataat tacggtgtag ttgattttgt
     5521 aaatggtata gcacctggag ttttcgtagt agtagcccat aaattaaaag cagttaatga
     5581 tgaattaaaa tatttaagta tgggtgatgg acctaattac atattatata gaccatatca
     5641 tttatgtagt ttagagactc ctttatcagc agctatggca gttttagacc ataaggctac
     5701 aatagtacct aaagctggac tagtagcaga agttatgact atagctaaga aagatttgaa
     5761 aaaaggtgag tttatggatg gctatggagc atatacatgt tatggtacta tagaaaaata
     5821 cgatatagca aaggctatga atgctgtacc tattggtttg ataagtaaaa agactaaagt
     5881 tgtaaaagat attaaaaaag gtgaagttat aacttacgat atggtagaga tagaaaaaga
     5941 tactacttta tatcatttaa gacaattgca agaaaaaata tttgggtaag atttgatggt
     6001 aatataaata gatattagta aaaagggttg atggttattt ttaaatcaac ccttttaaat
     6061 ttttatatat tcttgttagg atactttctt taaaagtata ttataggtta tttgtagtcg
     6121 taaaaattat tttgaattag ttctgcaatt ttatattcac attgaaataa tattattaaa
     6181 aattttaata taaaatgaat taaaatgacc aattatgtat tataatatta actgtaatac
     6241 taatatagtt ctattaatta aagaaatatc ccccattaat atataataaa tatctttaag
     6301 cgagagaact tagtaaaaaa tatatcgtat tcaataaaaa atctgtttac agaatggtat
     6361 atatagttat ataatatact atataagatg cgaaaagata aaaagtatag cacaatgaaa
     6421 gaaggtgatt attattcagc ttaatgaaag acaactaaaa attatagata tagttaagga
     6481 aaatgaacca ataacgagtg aaagtattgc ctccagcttg aatgtgactc gtgctacact
     6541 tagatctgat ttagctatat taacaatgac aggaatatta gacgcaagac caaaagttgg
     6601 atatttttat tcaggagtta gtgaaataaa tttaattggt aaaagtgtaa aagaaaaaac
     6661 agtagaggat ataatgagca tgcctgtact agctaagaag gatgaaagta tatatgatgt
     6721 aatagtcact atgtttttat ctgatgtagg gagtatagtt attatagatg agaatgaaga
     6781 gttatgtgga gttgtttcca gaaaagactt gctaaaagca actataggag gttctgatat
     6841 aaataaaatg ccaataggta tgataatgac aagaactcca aatgtagtca ctttaactaa
     6901 aggagcaagt gttttattag cttcaagaaa aataatagaa catgaagtag attcgattcc
     6961 gattgttgaa tataagggag aagacaaaaa tcatatgaga gttgttggga gaatatcaaa
     7021 aaccaacata actaagctat tcttagaaat agttgacaat taaggaggta tgctggtgaa
     7081 aaacctaatt atatatgcag tatcagattc agtaggggag actgcacaac aagttgcaaa
     7141 ggcatgtatg tctcagtttt atgtgaatga aacttatgaa ataaaaagat ttccatacat
     7201 gattaataaa ggagtactac ttgaaacttt ggagaatgca aaagctgaaa acgctttaat
     7261 tgtatataca ttagttgatg aagaattatg cagtattgtt gaaagatatt gcgaaagaga
     7321 aggtcttagt tgtatagatt taatgacaga tatactaaga gaaataagca agagaacagg
     7381 aagaaaacct aaaagagaag ctggtataat tagaaaatta gatgaatctt actttaagag
     7441 agttgaagct atagaatttg cagtgaaata tgatgatggg aaagacccta gaggtgttct
     7501 tcaagcagac ataattttag tgggaatatc aagaacatct aaaactcctt tgagtatgta
     7561 tttagctaat aaaaatataa aagtagctaa tgttccatta gttccagaaa taccaattcc
     7621 taaagaagtg tttgagatag atactaaaaa gataataggt cttactaatt cacctgaaaa
     7681 gctgaatgag ataagaaccc aaagattaaa agctttagga ttgtccagta aagcaaacta
     7741 cgctaattta gaaagaatac ttcaggagtt agattattca gaagagataa tgaaaagaat
     7801 tggatgccca gtaataaacg tttcaaataa ggcaatagaa gaaacagcag gtataatttt
     7861 agacataatg aaagaaaatg gtctaaagat atacaaagaa atagaaattt aataatgtct
     7921 attaataaaa atgatataaa ttaaaataaa aagattaatg ggggatttaa caatggaaac
     7981 taagtatgtt tatagttttg gtgaaggaag caaagacatg aaatctttac taggaggtaa
     8041 aggtgccaat ttagcagaaa tgactaagat aggcttacct gtccctcctg gattcactat
     8101 aacaacagaa gcgtgtaatg attattatgt taacaatgaa agtataagag cagagataat
     8161 aaaagaaata gaagagcatt tagctacttt agaaaaagat ttaaataaaa cattaggatg
     8221 caataagaat ccattattag tatcagttcg ttctggtgca gtattctcta tgccaggaat
     8281 gatggataca attcttaacc taggtcttaa tgacaatagt gttgttggct tagcagaggc
     8341 tacacaaaat gaaagatttg catatgatag ttacagaaga ttcatacaaa tgttttctga
     8401 tgttgctatg gaagttccaa aatataaatt tgaaaatgta ttagacagag taaaagaagc
     8461 aaagggatac acagttgata cagaacttac aactgatgat ttaaaagaaa tagtaaaaga
     8521 atttaaagca atttataaaa aagaaataaa aaatgatttc ccacaagacc caaaagagca
     8581 attaatgctt gcaatagagg ctgtatttag atcatggaat aatcctcgtg caatcgtata
     8641 ccgtaaattg aatgatatag ctcataactt aggaacagca gtaaatattc aatcaatggt
     8701 atttggtaat atgggagaaa ctagtggtac aggtgttgca tttactagaa atccagctac
     8761 aggagaaaat aaattatttg gtgaattttt aatgaatgca caaggtgaag acgttgttgc
     8821 aggtataaga actcctcaaa atatatcaac tcttgcagat gtaatgccag ctgtattcga
     8881 tgaatttgta aaaattactc atatacttga aggtcattat aaagatatgc aagatataga
     8941 gtttactata gaaaatgaaa gattatatat actacaaact agaaatggta agagaacagc
     9001 agcagcagca ataaatgtag cggttgattt agttgaagct ggaataattg atgaaaaaga
     9061 agctatcatg agaattgagc ctaatcaatt agaccaatta ttacatccaa aatttgaaga
     9121 taaggcatta aaagaagcta aggttatagc aaaaggttta ccagcatcac caggtgcagc
     9181 aagtggtaaa gtttatttca atgctgatga tgtagtaaaa gcaaatgaaa aaggagaaaa
     9241 agtagtttta gttagactag agacatctcc agaagatata gaaggaatgg ttaaagcaga
     9301 aggaatcctt acagctagag gtggaatgac atcacatgca gcagttgttg ctagaggtat
     9361 gggtaaatgt tgtgttgctg gatgtggaga aataaaagtt gatgagttta ataaagaaat
     9421 aagagcactt gatgatgtag ttataaaaga aggagaatac atttctatag atggttctac
     9481 aggaaatgta tacctaggag atgttaagaa aacagaagta tctttaactg gaaattttga
     9541 aaaattaatg aactgggtag acaaacacaa atgtatgatg gttagaacta atgcagataa
     9601 tcctagagat gctcgtgcag ctatagagtt tggtgcagaa ggtataggtc tatgtagaac
     9661 tgagcatatg ttctttgatg aagctagatt gccagcagtt agagaaatga tattatctaa
     9721 tacagtagaa caaagagaaa aagctcttga aaaaatatta ccaatgcaaa gagaagattt
     9781 tgtagagtta tttaaagtta tggatggaaa accagttaat ataagattac tagacccacc
     9841 actacatgaa tttttgcctc atgatgatga gactatagaa gaactttcaa aatctatggg
     9901 aataaaagta agtgatatta agaagagaat agttgattta gatgagttta acccaatgct
     9961 tggacacaga ggttgtagac ttgcaataac ttatcctgaa atatgtgtaa tgcaatctaa
    10021 agctataata caaggtgcta tagaagcaat caaagcaggt gttaaagttt ctccagagat
    10081 aatggttcca ttagttggag aagtaaatga gttaaaaata ataagaaaaa tgatagtaga
    10141 aactgttgac gctataataa aagaagaagg tatagaagtt ccttatactg taggtactat
    10201 gatagaaata cctagagctt gtttaactgc tgatgaaata gcacaagaag cagacttctt
    10261 cagttttggt actaatgact taactcaaat ggcatttgga tattcaagag atgatgctgg
    10321 taaattctta ggtcaatatg tagatgaaga aatattagaa aaagacccat tccaagtact
    10381 agaccaaaat ggtgttggta agttagttaa gatgggagct aaattaggta gagaagttaa
    10441 accagaatta aaactaggaa tatgtggaga gcatggagga gaaccatctt cagtagagtt
    10501 ctgttatagt gtaggactta actatgtatc ttgttcacca tttagagtgc caattgctag
    10561 acttgcagca gctcaagcat ctataaaaaa tcctagataa taatagactg caaaatatgt
    10621 aactaaataa aaataaaatc ccctattgtt caatgtatca aattaagtat ttgtaaattg
    10681 aatatagggg atttttatta taaaaattta acttatatag ttgtaagtga aacgtattta
    10741 attatattgc atatattatt gtagagagaa atttcaacta tcaattaaat tgagtttact
    10801 ggaggggata aagttgacaa agtgtcctaa atgtggaaag gaagtatcat cacgtccagg
    10861 tagtatatgc ccacgatgtg gatttaaagt agcagatgag agtctaaaaa taagatgtcc
    10921 agaacctagt tgtagggctt tggtatctag gaaactggat tattgtccta aatgtggatg
    10981 caaacttaga ggatataata tttctgaatt tgttaacatg gtaagatgta aaatagatga
    11041 gacatttgat aaaaaataat taaatttaga gttaagcttt tgagggtgtc tcaaaatgaa
    11101 ctttttagtt cataaggcac tctttttgta tggaattaaa tatattttta taatttaaac
    11161 aataaaaaag aggcttatct ctattttgag atagcctcta taaaattata cagttttata
    11221 agcaaatata tttttactaa tctatttcta ttatttgcct tctgaatctt ccatgactaa
    11281 agccacgagt gtgcataact attttttaat caaacgtatg ttaaagtaat ctttctattt
    11341 accagaatta ttttccagta ttaagtatcc aaggtttttc tataccattt tttagtgagt
    11401 tataattcac aataagagtg tcatagctat ttttagcatc ttcattactt accacttgtt
    11461 ttttataatc agttagagta attaatccta aatcatatct taatttagta ttttttaatt
    11521 gtttttctga taaatcaagt tgttttttag taatctgaat tgtatcttct aaagcaagta
    11581 attgagcata gctatcaatt aacccttttt taagagcttt tttactgtct tctaatgtta
    11641 ctagaccatt ttcagtatta aatttttgtt gaagataatt tgcataagta gtaagaccag
    11701 cagcataagt ttttctttca ttgttgtact cagtaacatc tattttatat tgctcgtaat
    11761 cctttttata cttatccata agaacctcat attcatcatc agttaggtta ccttgctctg
    11821 gttttgtagg agcaactggt cttgtaagac taggtctatc tggtaaatct gctttgtact
    11881 catctttatc attaaaactt tcttctgtac gttctaaaat caattggtcg tattttaaat
    11941 attcattaac cttaccttcc atatatgaat caactgagcc acctactcta aaagactcaa
    12001 atctatattc tttatccaat tgataattat ttaaatctat attagtcaac aacttaaaat
    12061 agtcttgatt gttttttagt tcttgaagtt tagctttttg agtattttgt agtgtttgaa
    12121 gttcaagttc agctgattca gtttctaaag atgtcactaa ccctaaatct ttttttaatt
    12181 tcatatcttt aatttcttta gtctttatct caagattgtt ttttatctta tctagctcat
    12241 tttctcttga aactaaatcg ttaaatttat tagtaatatc atttgctatt tggtcctcca
    12301 taaagccttt agcctgttca ctttgatttt taagaagtcc aatttggtca tatggaaatg
    12361 aattgctacc ttcactatat ctatcaattt catcttgtac ttctaacttt tcttttaaca
    12421 tttttatttc ttctgatttc atttttaatt tttcactatt tttaagtgca gcactaacag
    12481 cttctctagc agtcaaaatt tccttttcag atgttctttt atttgagtca cttaaaatag
    12541 aattatcaac tttgtttata gaagaagtat tattatctgc gtaaactggg attaaattgg
    12601 cagttattat accaacacca cagccaattg caactatttt gctcattttt ttattcaata
    12661 caatcaccct ccattctatt taaactttag ctttcaattt tggtttcttt attattttac
    12721 gttttatact tcctatttta tctgttatct taccaaatat tttaggcatt ttgtctgaaa
    12781 acttattttc ccatttatca aatattaagt atatagtagg tataagcacc aaagttataa
    12841 gtgtagatac agttagacca cccattataa ctatggctag tggttgcata gtttctccac
    12901 cttcaccaag agatagtgct gttggcaaca tacctaaaat tgttgttgct gtagtcatta
    12961 agactggtct taatcttgct gcacaaccaa ttgaaaccaa accaattaaa tcattgttac
    13021 gatttgtttt tctaagttgc tctatgtagt caataagaac tataccatta ttaacaacaa
    13081 taccgataag taagattata cctagcatac cgacagtgct taaactttga ccactaacta
    13141 gaagcgaaag cacaactcca acgaatgcaa atggaacaca gaacatgatt ataaatggtt
    13201 tactaaatga ttcaaactga gctaccatta ccatataaac taatgctata gctatgacca
    13261 ttgaaaatcc aagacttgcc atagattcat tcatcatttc tgtttcacca ccaacaccta
    13321 tagagtaatc tttaggaatt accattttat tcgttttttc tagtgctagt tttgtagcag
    13381 taccagcatc taaattgtca agtgtagcag ttatatttac agaaaaatct ccatcagtcc
    13441 tagatataga ttgtagacca tctgccattt ttatatctga aaaagcacca agaggaactt
    13501 cttttccagt tgcagagttt acttttagtt gctttatatc ttctaagcta tcaatagtat
    13561 ctgctttaaa ttttaggttt acatccactt tgtaatcatc aatagtagca gtggttacgg
    13621 agtcaccatt tattgctgta cgtaacatgc ctgcaatttc agcagtattt acaccatatt
    13681 cttgagcctt atgtttatct atagtaaatt gagcttcttg tgttgtatct gtaagagaag
    13741 tttcaacatt tctaaatcct tttatagagc taaattcttt ttcagcttct tttcccagta
    13801 tttttaatac atctaaatca ggacctttca tttctaatgt gaagtctgct cctccagctc
    13861 cacccataac actactgttt tgacttacat ttattttaca atctggtaca gtagccactc
    13921 tatcaattat ctctttttct atttcatcag tagttctttt tctatttttt tgagattcta
    13981 attcaattgc tatacttgct ttactattat ttgaatctga tgtcaaagtt gttattaaag
    14041 ttttaatttc tggaatatct gagacttttt tctcagtcat agatacatag taatcattgg
    14101 attctaggct aagacctttt ggaagttcta tagaaataga taattcaccc ttgtctgctg
    14161 caggcataaa gtccatacct ataaatccca ttccaaataa ggaagctaca aaaataagta
    14221 aacttgatat tacaactaag cgttgatgtc tcaaacatac acttaataat tttttatatg
    14281 cttcacttac cttatcaaat ataggactat ctttttcttg catcttttga ctattttttc
    14341 ctctgcttaa ttttgcaaat acactaggaa cgagtgtaat tgcacctata aaggagaatg
    14401 ctagtgaagc tattagtgtt ttacctaatg ccccaaaagt tattttagct ataccttctg
    14461 taaacatgat aggtaagaat attgctatcg tggtcaatgt tgatgcaagt attgcatttg
    14521 ttacagttct tgttccctca atagcacaat catcaagact caatctgcca cttttcctat
    14581 atttaaatat attttcaatt acaactgtag cattatcaac aaccatacca actgaaatta
    14641 ccaatgaact tgcagtaata agatttaaac tttcaccagt aaagtagaga attgctatag
    14701 cacctattat agaaattggt attgcaactg caacaactag tgaagctctt acacttttta
    14761 ggaaagcaaa tattacaata aatgcgataa cagcacttat aagcaagttg ctcattacac
    14821 tgtttattga ttcacttatg tatgaacttg tatcattagc aattttaatt ttaaattgag
    14881 gattttcctt attcaattca tctacgtatt tgtatgcact ctgcataact tttacagtgt
    14941 tggcatcttg ttgtttagaa atatctatta ccaaactgtc ttttccattg tatctataaa
    15001 tactatcttt gtctgcatat ccataatcta cactacaaag attatctaat ctaactgtat
    15061 ttcctccttt tacaggaatt tgaatctgtt ttatatcatc tagggattct aatttatcaa
    15121 ttgctcttaa aactatttta tcctcacctt gagtaattga accataaggg aatgttttat
    15181 tactagagga taagacacct tttatagtgg ataaactaac tccataattt gaaagaacag
    15241 ctgggtcagc tattacatta acctgtgctt tttttccacc tttaaggtct gctgaagtaa
    15301 caccatctat ggattctatt ttaggctgaa ttacatcttc agcgtatttt cttaaggctt
    15361 gtgaactatt ttcatttcct ccagatatga caagttgacc tatagcctgt gcattcatat
    15421 ccagctttaa aactgttggt ttagctgcat catctgggag gctggtctgt aatgcatcaa
    15481 ctttagacct aacatcattc attatttcat ccaaattagt tccaaattca aactgagcaa
    15541 ctaccataga aacactttct tgtgaacttg atatagtaga ttttacattt gaaacggcag
    15601 ataaactttc atcaactttt tgactaactt gttcatcaac gtcttgagga ccagcacctg
    15661 tccaagtagt catgaccatt acaactggta tttctatatc aggcattaga tttattgaca
    15721 tttttttata tccaatacca ccaaaaacta ttaatactaa aaagaccatt atgattgtga
    15781 gaggacgttt aacggaagtc tgtgttaaat tcatagtcta cttctccttt tctacaggaa
    15841 atatcttagt tccgtcataa ataagactta tccctccaat aatcactgta tctttatcac
    15901 ttacaccact tttaatttct attttatcct ctgttacaat tccagtttga accaagtgtt
    15961 tctttgctat attgtctttt gttgctatgt atacatattt tttaccattt tcttcaaata
    16021 ttgctttttt aggtactgta acagcaccat tttctttttc aacacttaag tttacttgag
    16081 cagacatacc tgccttaaat ttgttgtttg tattatttac aagtacttta acaggatata
    16141 aagatgtctg agggtcaact actgaaggta cataatctat ttttcctaaa atcctttggt
    16201 cttcaattat aacatctaca tttccatctt tcttaaattt acctatatct gattctgcta
    16261 catttaaatc aacttctagt atattaggat tagaaataat aaaagctggt tgtgattgtg
    16321 ttatcatttc ctttgaatca aagtttttag ctgttattgt accatctact ggagatgtta
    16381 atgttaattt gtctaaatta ctcattgcta aattatatga tacttgagct tggtcaagct
    16441 gttttttagc aagtgcttta gcttgaggta tactttttga agttgctgta tcgtatgctc
    16501 tttgagcaga atctaactgt tgttttgttt gctctaaaga aaattctatt tttttaaatt
    16561 ggtctaaact aatgacttcc tgttcataaa gaatcttatt attgtcataa tttctttgag
    16621 attcctcata ttgaatcttt gcattgtcaa gagctgtttt tgcctgattt acttgtgcct
    16681 ctaatgaacc accagtggca cttgaataac ttgcttttgc taaatctagt gaggctttac
    16741 tttgctttac actattttct agctctggac tgcttatact caataattca tctccttttc
    16801 ttacattttg cccaagtttt acatacactt cttgaactgt gccaacagtc tgtgcagtaa
    16861 cagctgtttc atctttaacc tttgtagttc ctgagaaaga atttgtgttt tcaatttctc
    16921 cacctactgc tttttgaaca gaaactgcta ttggtttctc aggttcagat tcttctgttt
    16981 ttccacatcc aactaaaaac agtgaactac acattaagaa aactattaaa tatcttttca
    17041 tatctcctcc tattatatat tgcacactag cttgttgtag aatttaaagt gtcttataat
    17101 ttaagtttag ttttagacta attaaaaaac ttccctttat tggcaattta taattctcta
    17161 atttgattat aaatttataa ttattatgag tcaataactt tttcttgttt taaatttatt
    17221 aatagtttat aatttatact atattaaaca acaatgtttt attatattat ctattgaagg
    17281 gatgttacaa atatggataa caacaatata cttaaaatag cggactcttt tttagatttt
    17341 ttatttgtgc ttgaaaataa tatatttaaa gaaagcgatt tgttaaaaaa gtttcaaaat
    17401 aactctgata taatgaaagc ttattttgaa gaatgtccaa tggctccatc acatgctaag
    17461 gtaattcttt atcttatgac atcaaattca tcatcaattt ctcaaatagc ttctaattta
    17521 ggaattttaa aatcaaatat gactccgatt attgacagat tagtggaaca tggtttagtc
    17581 aataaatttc ctgacccaaa agatagaagg attcttaggg ttgaattaac ggataaagca
    17641 tttaaattat ttgatgcagt aaaagctatt ctcaaagaat ctctagtaaa aaaattatct
    17701 aatctttctg aagaggaact aactttatta gatgaacata cattaaagtt atcagaaata
    17761 gtaaagaaat taggttagag tgtgattgta aaagtcacta ttaaaaaagt taaaac
//
LOCUS       DAEMHZ010000079        17664 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000079 DAEMHZ010000000
VERSION     DAEMHZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.079"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..546
                     /locus_tag="KPK72_002420"
     CDS             82..546
                     /locus_tag="KPK72_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG2489628.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            553..1047
                     /locus_tag="KPK72_002421"
     CDS             553..1047
                     /locus_tag="KPK72_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG2489629.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1063..2088
                     /locus_tag="KPK72_002422"
     CDS             1063..2088
                     /locus_tag="KPK72_002422"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG2489630.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2075..4522
                     /locus_tag="KPK72_002423"
     CDS             2075..4522
                     /locus_tag="KPK72_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG2489631.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4702..6075
                     /gene="radA"
                     /locus_tag="KPK72_002424"
     CDS             4702..6075
                     /gene="radA"
                     /locus_tag="KPK72_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG2489632.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6078..7148
                     /gene="disA"
                     /locus_tag="KPK72_002425"
     CDS             6078..7148
                     /gene="disA"
                     /locus_tag="KPK72_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG2489633.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANTQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7237..8331
                     /locus_tag="KPK72_002426"
     CDS             7237..8331
                     /locus_tag="KPK72_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG2489634.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8460..10637
                     /locus_tag="KPK72_002427"
     CDS             8460..10637
                     /locus_tag="KPK72_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489635.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10816..11149)
                     /locus_tag="KPK72_002428"
                     /pseudo
     CDS             complement(10816..11149)
                     /locus_tag="KPK72_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            11530..12483
                     /locus_tag="KPK72_002429"
     CDS             11530..12483
                     /locus_tag="KPK72_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG2489636.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            12521..13483
                     /locus_tag="KPK72_002430"
     CDS             12521..13483
                     /locus_tag="KPK72_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG2489637.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            13508..14494
                     /locus_tag="KPK72_002431"
     CDS             13508..14494
                     /locus_tag="KPK72_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG2489638.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            14507..15553
                     /locus_tag="KPK72_002432"
     CDS             14507..15553
                     /locus_tag="KPK72_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG2489639.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            15720..17450
                     /gene="lpdA"
                     /locus_tag="KPK72_002433"
     CDS             15720..17450
                     /gene="lpdA"
                     /locus_tag="KPK72_002433"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2489640.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNISKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEVDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     ELDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEIASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT         7327 a         1800 c         3373 g         5164 t
ORIGIN      
        1 ttagggcttt aaaaatttaa ataataggtg taaaataata gtcaaagaaa gtcaaaaata
       61 aaaaggtgtg tggtgatatt aatggcaact atgacagata taattgaaaa atttattaaa
      121 gatttgatgg aagaagataa tagcatacaa attcaaagaa atgaactagc aaacttgttt
      181 agttgtgcac catcacaaat aaattatgtc ttaacaacaa gatttacaat agatagagga
      241 tattacattg aaagtaaaaa aggtggcgga ggatatgttc aaatagaaaa aatacgaaaa
      301 agtaaagatg ggcatattag agaactacta aatgaaaaaa taggaagtca gatatcctat
      361 aaaaaagcaa aggaattatt agaaggttta aaagagtcgg atttgattaa cgaaagagaa
      421 ttaaaattaa tattatatgc aatagatgat aaatcattgt gtatgccaat atatgaatta
      481 aaagagaagg taaggtctaa tattttaaag aatataataa taggattatt tagtatagag
      541 gagtgataat ttatgctatg tcaaaaatgc aataagaata aagcatctgt atattataat
      601 aagatagtca atggagaaaa aacagagatg tatttatgta gtgagtgtgc aaaagagaat
      661 acagaaatga attttaactt agatatgcct ttttcaatga tggatatatt ttctaattta
      721 ggttttcaac ctaaaaaaga acttgaggaa aagctagttt gtcctaaatg caatacaaca
      781 tatagtgagt ttaagaataa tgggagattt ggatgtagtg aatgttataa tgcatttagt
      841 agtcaagtaa atccaatgct tcaaaatata catgggcata tagaacatac aggaaaagca
      901 cctaaaaaat ctttttataa gataagtgta gaaaatgaga ttaaagaact aaaagaagat
      961 ttagatagag ctataaaaaa tgaagagtat gaattagcag ctcaatttag agataaaatt
     1021 aaatatttaa aaggttctat tgactaagat tggagtgata ttatgaaaga gaacatagtc
     1081 atgaaatcaa gagttaggct agctagaaat ttaaataact atccatttcc aaataaatta
     1141 gataaagaat gtgccatgga aataatagaa aaagtaaaaa atgcgtttat taattctaac
     1201 ttagaacaaa aagaagaatt tgatttctat aaaattgaag atttagacca atctaaaaaa
     1261 atgcttatgg ttgaagaaca tataataagc ccagatttag cagaaaatga taaaagtgca
     1321 gttatagtaa aaaaagataa aactataagc ataatgataa atgaagaaga ccatataagg
     1381 atacaaacta tatgtgatga tttaaactta gaatatgcat atagtgtagc aaatgaaata
     1441 gatgatttgt tagaatcaag tttagaatat gcctttaaca caaagctagg ttatctaaca
     1501 tcttgtccaa ctaatacagg aacaggaatg agagcttctg taatgatgca tttaccagca
     1561 ttaagtcaac taggctatat ggatgaactt tataaaattt catcacaaat aggtatagca
     1621 attagaggga tatatggaga acgaacagaa gcattgggaa atatatatca aatatctaat
     1681 caacttacac ttggaagaac agagtctaat attatagaaa atgtaagtgg acttacaaaa
     1741 gatgcaattt caaaagaaat aaaagctaga gaaatattgc aaaaaaaatt aggtataaaa
     1801 ctagaagata aaatatttag atcgataggg acacttgaaa attcaagagt aatgagtagc
     1861 gcagaggcaa tgagccatct atcaaatata aaaatgggaa tagaaatgaa ctatatagat
     1921 aaactagatt taagggctat agaacaactt atgataggta tacaaccagc acatcagtca
     1981 ataatgtata agagtgatga tgttgagaat agagatataa atagagcaac atatattaga
     2041 gaaactttgg aaaaattaag aggagggatg aattgtgaac ttcaatagat ttacacaaag
     2101 agctaaaaag gcaattgatt tagcatttga atctgctaaa agtctaggac acaatattgt
     2161 cggaagtgaa catatacttt taggtctttt aagagaagaa gaaggtatag ctgctaaagt
     2221 tctaagtaaa gttggattta cagaagctta tttagaaggc aaaatagttg atatggaagg
     2281 taagggagaa gaaatctcag aagatatagt attaagtcca aggagtaaac aaatacttga
     2341 attatcagga atgtttgcaa ataaattaaa aacaaattat attgggactg agcatatatt
     2401 attagcaatt attcaagaag gtgaaggaat agctaataag attttaaatt atgctggagt
     2461 aaatgatagg actttagccc aattaacaat tgatatgatg ggtattagtg ataaaaatca
     2521 atacaaagca gaaaatagtt acacgggcaa ccagaatcaa gcagaatcta aagtacttga
     2581 taagtatgga agaaatctta cactatatgc aaaacagaat aaaatagacc ctgtaatagg
     2641 tagagaaaaa gaaatacaaa gagtaataca aatattaagt agaagaacta aaaacaatcc
     2701 agtgttgata ggagacccag gtgtagggaa aacagccata gctgaaggtc tggctacaaa
     2761 tatagcctta ggaaatgttc cagaaacact aaaaaacaaa actttatact ctttagagat
     2821 gggttcatta ttggctgggg ctaagtatag aggagaattt gaagaaagaa ttaaagaagt
     2881 tgtagatgaa gttgttaaaa atggaaacat aatcttattt atagatgaga tgcacacaat
     2941 aataggggct gggtctactg gagaaggctc tatagatgct tcaaatatat taaagccagc
     3001 cttagcaaga ggagaaatac aagttatagg tgcaactaca attgatgaat atagaaagca
     3061 tgttgaaaaa gattctgctc tagaaagaag attccaacca gttatggtag atgaaccaac
     3121 taaagaggac tcaataaaaa tattagaagg attaagagat aaatatgaag ctcatcataa
     3181 agttaaaata actgatgatg ctataaaggc agctgtagaa ttatcaacta gatatatatc
     3241 agacagatat ctgccagata aagctataga cttgattgat gaagctgcat caaaagtaag
     3301 attaaaagaa aatacacctc cttcagaaat aaaaaaatta gaactagaaa tagaaaatat
     3361 agataaagaa aaagaagaag cagtaagatg tcaagacttt gagaaagcag ccaaaataag
     3421 agatgaacaa ggtctactta aaaaacaatt agaagatgtt agagaaagat ggaacaagtc
     3481 atctaaacat tcagatttag ttgatggaga agttattgct gaagtagtgg gcttatggac
     3541 aggaatacca gttaataaaa ttcttgaaga agaggctgat agacttttga gacttgagga
     3601 aatactgcac aatagagtta taggtcaaga acaagcagta aaatctattt caaaggcaat
     3661 cagaaggtca agagcaggtc ttaaagaccc taatagacca attggttcat ttttattttt
     3721 aggtccaaca ggagtaggta aaacagaatt atctaaggca ttagcagaag tgcaatttgg
     3781 agatgaaaat caaataatta gaatagatat gtctgaatat atggaaaaac atgctgtgtc
     3841 aagaatgata ggttcacctc caggttatgt aggtcatgat gaaggtggtc aattaactga
     3901 aaaagtaaga agaaatccat attcagttat cttatttgat gagatagaaa aagctcatcc
     3961 agatgtattt aatattctgt tacaaattct agatgatggt agactaactg attcaaaagg
     4021 aagaactgta gattttaaga atactattgt aataatgaca tcaaatgttg gagcatctac
     4081 aattggtaga caaaaaactt taggatttag tatagctaaa ggagacgaag aagaaaaatc
     4141 tcaatatgaa aaaatgaaag aaaatataat gggtgaatta aaacaaagat tcagaccaga
     4201 gtttttaaac agaatagatg atataattgt tttccactca ctaaatgaaa atcatatatc
     4261 aaaaatagta cttttaatgg cagcgaaact acaagagaga ttaaaagaaa tggatataaa
     4321 attagaaatg agtgatgagg ctgttaaatt aatttctaaa tctggatttg acttagaata
     4381 tggagcaaga cctcttaaaa gagctttaca aaaagagttg gaagatgagc tatctgaagc
     4441 aatcttaaaa ggtaatgtga aaaagggaag taatgtagta gcaaaagtca aagatgaaaa
     4501 aatagttttt gaaactaaat aatttaatag agttgcattt taaaatattg caaatataag
     4561 ttttataatc taaacaaaat tttaagggca atgaatctat aataattcat tgtccttaat
     4621 tattttattt aatctttaca tattgtcttt cagatatgct aaaattatag tatagaataa
     4681 aaaattaagg atgataaaag tatggcaaaa ataaaaacaa aatatgtatg tcaatcttgc
     4741 ggatatgaaa cagctaaatg gcttggtaaa tgtccagaat gtacaaaatg gaatacattt
     4801 gtagaagaaa tagaccaaaa aagcaccaaa aaagaggtct ttataataga taaatcttca
     4861 tctaaaccag tcagtataaa ctcaatagaa agtaaagaag aagagcggtt tacaacagat
     4921 ataaatgaat tagatagagt gcttggagga ggtatagtta aaggctcttt agtgcttgtt
     4981 ggtggtgacc ctggtatagg taaatcaaca cttttaatcc aagtatctag taatgttgcc
     5041 aatttaggaa aaactgtttt atatataact ggagaagaat cagaatctca aataaaaatg
     5101 agagctaaaa gattaggtat taactcagaa aatttatata tatttgctga aaataattta
     5161 agtataattg aatcttattt agaaagtgta aatccagaat taataattct agactcaatt
     5221 caaactgtct tcagtccaga aatatcctcg gcaccaggaa ctgtcagcca aattaaagaa
     5281 ggtacttcaa aattcatgaa aatttctaaa aaaatgggaa tttcaacgtt catagttggg
     5341 catgttacaa aagaaggttc actagcagga ccaaaattat tagagcatat ggttgatact
     5401 gttctatatt ttgaaggaga aagatataat acttatagat tagtaagagc tgttaaaaat
     5461 cgatttggtt caactaatga acttggagtg tttgaaatga gggatttagg attggtagaa
     5521 cttgataatc catctaaaat tttaatctct gaaaaaccaa aagatgtagc aggttcagta
     5581 ataatatcta ctgtagaagg tacgagacca atgttacttg aattgcaagc attagtttct
     5641 cctacaagtt ttggtatacc aaaaagaaca tctacaggag tcgattacaa tagagtaggt
     5701 atgttattag cagtattaga aaaacgtgtt ggtttgcaaa tacaaaatca agatgtctat
     5761 ataaacatag ttggaggaat aaagataaat gaaccctcaa ttgatttagg gatagccatt
     5821 tctgtagcat ctagttttag aaatataccg atagatgaag atatagctgt tacaggagaa
     5881 gtgggactta caggagaagt tagagcagta agctttatag aaaagagaat agctgagtgt
     5941 aaaaaacttg gatttaaaaa gatagtagtt cctagaagta attatgatgt tgtaaaagag
     6001 accaaaggca tagaaatatg gccagtagat aacttaagac aagctataaa tatagtactt
     6061 gggaggaatc aataaatatg gagaattttc tagataataa aaatatgcta tatgcattaa
     6121 aaatgatatc tcctggaact ccacttagat taggtctaaa caatgtacta agagctaaga
     6181 ctggtggatt aattgtaatt gcaacaaacg aagatgtaat gaaaatagta gatggaggat
     6241 ttgctataaa tgcagaatat tcaccatcat atctatatga attagctaaa atggatggag
     6301 ctatagtttt aagtggtgat gtaaagaaaa tattatttgc taatacacaa cttatacctg
     6361 actattttat agaaacatca gagacaggaa caagacatag aacagcagaa agagtagcaa
     6421 aacaaactgg tgctatagtc ataggaattt cacaaagaag aaatgttata acagtttata
     6481 gaggaaatga gaagtatgta gtcgaagata tatctaagat atttactaag gcaaatcagg
     6541 ctatacaaac tctggaaaaa tataagacag tattggacca agctgtaaca aatttaaatg
     6601 ccttagagtt taatgatttg gtaactattt atgatgttgc attagtcatg caaaagatgg
     6661 aaatggtaat gagagttaca agtataattg aaaaatatgt gatagaattg ggtgatgaag
     6721 gaactttagt aagtatgcaa ttagaagaat taatgggtac aaccagaata gaccagaaat
     6781 taatattcaa agattataat aaagaaaaca cagaaataaa agaacttatg aaaaaggtca
     6841 aaaatttaaa ttcagaagaa ctaatagaat tggtcaatat ggcaaaacta ttagggtata
     6901 gtggtttttc agaaagtatg gatatgccta taaaaacaag aggttatagg attcttagca
     6961 aaatacatag actaccaaca gcaataatag aaaacttagt aaattatttt gaaaactttc
     7021 aacaaatttt agatgcatct attgaagaat tagatgaggt tgaaggaata ggtgaaataa
     7081 gagcaacata tataaaaaat ggactcataa aaatgaaaca attagtctta ttagatagac
     7141 acatatgatg aattttaaat gaattttact acttttttct tgaatataat atacactttg
     7201 gtctataata gataagtata actagggggt gaagcattga taaggaaagt aacaagaata
     7261 ttgtttattt tacttggatt tacaataggt ataacaacct atttaacatt aatgaaagat
     7321 tttgaaatat taacatttgg aaaagaaaca tatggttata tcgcagctgt agtagcaggt
     7381 ataataatag ccattttagg atatcttata gaaccatggg ttgtaaataa agtaaaagaa
     7441 attgctaaaa tagtggataa agaattgtca aaatatcctc agactgatat attgttaggg
     7501 tctatgggtc ttatagtagg atttgttatt gcgtatttat taagtggatt agtaaataga
     7561 ataccaatag taggtggaat actatcctta ctattatatt tattcctagg atatcttgga
     7621 atgaaagttg cgcttaaaag taaaaatgat ttatttaata ttggaaaact aggaagatta
     7681 gcaaatccta ttaaggataa agataaggat aaagaaaata aaaaagaagt aaaagccatt
     7741 cctccaaaag tattagatac aagtgtaata attgatggaa gaatagctga tatttgtaag
     7801 actgggttta tagaaggaaa acttattatt cctgcttttg ttttggagga actaagacat
     7861 attgcagatt cttcagatga tttaaaaaga gtaagaggta gaagaggtct ggatatatta
     7921 aatataattc agaaagaatt aaatatagaa gttgaaataa gtgagagaga ctttgatgat
     7981 atagcagaag ttgatagtaa attattaaag cttgctcagg ttttaaatgg aaaagttgtt
     8041 acaaatgatt ataacttaaa taaagttgct caattccaag gtgttgaagt attaaatata
     8101 aatgagttag ctaatgctat aaaaccagtt gctataccag gtgaagatat ggttgttcaa
     8161 gtggttaaag aaggaaaaga ggctcaacaa ggtgttgcat atctagatga tggaactatg
     8221 atagttgttg atggaggaag aaaatatatg aatgagacta taaaagtact cgttacttct
     8281 gttcttcaaa ctcctgcagg tagaatgata tttgcaaaac ctaaaaatta atatgttcaa
     8341 agaaacctat aatttgggct gtttatttta aatagatggt ccaaatttat ttatttataa
     8401 cgataatgtt taatgtattc ttaaagatgg ggatttaaat cagaaagcag ggagaattta
     8461 tgagaaaaaa gattatatct ttcttagcaa catttatcat aatattaaca tcagcaagtc
     8521 agtatagttt tgcagatgat atatcaacac gtggaaaagt aatatttata gatatgaata
     8581 gaactagtat gagtaatatg ttaagaataa aatcattaag agaagaacta gacaatagag
     8641 ggtatattgg cttgatgaat ataagaggcg ataaaggaag tgatgataga aggtcatatg
     8701 ctagtatggg tgctggtggt agagctaatg tggctaatga agaagatatt aattttgaaa
     8761 gctcgagtaa ggatagaaat atagtttttg aatcagctac aggaaaaagt gcaaaaggta
     8821 taaataattt aactataaat aagtctataa atgaaaattt aaactttgga gagtatggtt
     8881 ctgtattagg gtcattagga caaagtttat ctgataatgg attaaaagca tctgtattgg
     8941 gtaattcaga tataattgaa aatggtcaac ttataaaaaa tagaaactta tgtctaactg
     9001 caatggatga atatggaaga atacctaatg gaaatgtaga tactataaat aaaaaagact
     9061 tatctatgcc ttatggaata tcaactgatt atgataaatt gattgtagag actaaagaag
     9121 cttacaaaaa taatgatgtg atttttgtgg agcttggaga cacatataga ttagatttat
     9181 ataaaccaaa tttaaatgaa aaaacatatg agagtatgaa agataacata gaaaaaaata
     9241 tagatatata tttaaaaaat atattttcta tggttgaaga aaatgacact gtctacatag
     9301 caagtgcatt tccaagtgat ttagattata aaaataaaag aagactttcg ccaataataa
     9361 aattaaatgg agaagggaaa ggtatattat catcttcaac tactagaaga gaaggtattg
     9421 taacaaattt agatgttggg gttgaaatat tagacaactt taatataaaa aatcaaaata
     9481 tggttggaag aaaatatgaa cttattaaca gagatgataa taaagaattt ttgatggatg
     9541 aatatcaaaa gatagtatct atttcaagca taaggtctac aatactgaat ggttttgtaa
     9601 gtatagtttt cttgtcatgg atagttgcta tgattgcaat tttatttaga aagcatatat
     9661 caaaaaatta taaagaaaca acatttttta tattaaagga acttttaaaa ataggaatag
     9721 taatgccact gtcatttatg ataacaccaa taatgaattt caaaacacca ttagcaatat
     9781 ctttaggaat aattataata acacttacta tatatcttat aagtaaagtt ttaataaaga
     9841 acgatttaaa aaacatgctg ttttttactg gattaaccat agtaattatg gttattgatg
     9901 ctgggtttgg ctcatattta atgaaaagca atgttatgag ttatgattgt ataattggag
     9961 ctagatatta tggagtagga aatgagtatc aaggtgtggc aatcggtagt gcaatattta
    10021 catttgcaat tttacttaca tataaaaata taccaaagtg gtctataata gttttttctt
    10081 tggttatctt aattacaagt gcatctccaa ttatgggagc aaatgttgga agtgctattt
    10141 ctgaatgtgt tgcattttta ctatttatac tattgatata taatgtaaag atagatttca
    10201 aaaaaatcgt attattaggt atagcagtag tatttgtatt aggagttttt gtagcaatag
    10261 atatgatttt gggaagtaat tcacatttag gcatgtttgt aaaagaaatt tactttaatg
    10321 gaccaggaga aataatacaa acatttagta gaaagataga gatgaattta aagctagctc
    10381 aaaccagtgc atgggtaaat atactgttaa ctggaatagg tgtaatttta gttcttatga
    10441 ttaatcagat tagatacttt aagcaactga tggatgagta tccaattgta tttaaaggat
    10501 ttatagcatc aatagcagga tgtttagtaa ctcttttagt gaatgactct gggattgtgt
    10561 catcagctac agcctttatt tatgtaattg tacctatgat aacattaagt ataaatctta
    10621 cagcattaaa agaataagta ctgtatgaat aatatgtaaa tattaataca ttaatttaac
    10681 ttctttattt acaaaataaa gaagaaagtt tataaaaaat aaattataat aaaaaagagt
    10741 ttatctcaaa attgattatt ctaatcaaat tttaagataa acttttttta ttataaagat
    10801 aaataacata taacttcata ttttaggtag attttttctt atatgtttag gagtgcattt
    10861 aaatttttcc ttaaaaattc tgtaaaataa ttttgtatta gtaaatccat gagcttctaa
    10921 tatactgcat aaggtatcat ttgtgttaat taagtcttta taaatatgag aaatacgcac
    10981 atcatttaaa tactataggt aaatttgtac atagactgtt taaatttttt tatagttatt
    11041 gtcattaaca gtgaccttaa taatatagca atttttaaaa tcaggaatgt atttacttaa
    11101 aaaagataag gggactttta atagtagtgg tattttcata agtacatcta tttttgaatt
    11161 tagagttgaa aatgggtcag gttcagcaat actacaaata ataaaaatat ctttttatga
    11221 gtttgacaaa aaatataata ctatacttaa tgaattaaat gttaaataat aaaaatattt
    11281 agcggacaat tgatttcgat tttatgaaga tgatatttag caattagaaa taatatataa
    11341 tacaaattaa gtagtaatgt tttagctgtt taaggacatg gtgctacaag aaaagtatca
    11401 cctttttttt gttatacaaa aaaacagttg actttttata tgaacccctt ctataatgtt
    11461 actgaaatta agtaaaatga ttgaacaaat gtaaagataa ttgaaaagct tgattttgga
    11521 ggaaagcaaa tgaaaaaaat tggagactta agattaatga tgaagtgttg ttcactttat
    11581 tacgaagata atttgaatca acaggaaata gcaaatcaat tgggaatatc aagaccaaca
    11641 atatcaagaa tattaaaaga agcttttgaa caaggaatag taaaaataca gatagttgat
    11701 gttttgaaaa atgattacca aaaaatagag agaagtttgg agagaaaata taaattaaaa
    11761 gaagtaatag tagtagatga taaacaggat gcactaacac aaaaacaaga actagcaaga
    11821 gctgtatctg aatatttaac tagagtagta aaggaaaatg atataatagg tgtttcaata
    11881 ggaacaacac taaaagaaat accaagatat gtggaaaaaa gtaactgtaa aaatgttaca
    11941 tttatacctc tattaggagg gattggagac aatgaaatag atattcatgc aaatcaaata
    12001 gcagtaagtt tagcgagggc ttttggaggt gattttaaat tattacatgc tccagctgtt
    12061 atgtctgact tgtctacaaa agagaaactt tgcaaagacg aaaaaattaa agaagtgcta
    12121 gatttaatag ataaaacaac tatagcaatt gtgggtatag gaaatcctat gagtttaaac
    12181 tcaacaataa tggcttctgg atacatgaat gaagcagata tagaagattt aaaaaaatac
    12241 aactcaatag gtgcaatttg tttacaagcc tttgataaag aaggaaaaac ttctatactt
    12301 gaatttaatc aaagagtatt aggagtgaaa ctggaagatt taaaaaagat aaaaagaaca
    12361 ataggtgtcg cttcaggaga tgaaaaaata gaggcaataa aggcatcatt aaaagcgaaa
    12421 tttataaata gtttagcaat taatcacagt ttagcactta aattattaga agattgtgac
    12481 taatataaag atagataaaa gttaacagga gggaatgtat atgtcaatat caaaagaaac
    12541 attgcttgaa atgtataaga gaatgaatca ggctagaaaa tttgaagaga aagttagttg
    12601 gttttttgca agaggtatgg ttcatggaac aactcactta tctgtaggtc aagaagcgtc
    12661 aagtgtggcg gctgtaatgg ctttagaaaa aggagattta gtatcattaa ctcatagagg
    12721 acatagtcaa tttataggaa tgggaataga cctaaataaa atgatggctg aacttatggg
    12781 taaagaaact ggattttgta agggaaaagg aggctcaatg catatagcag atattgagtc
    12841 aggtaactta ggagcaaatg gagttgtagg tggaggtctt acaatagctc caggagctgc
    12901 acttacacaa cagtataaaa aaacaggcaa aatagtttta tgtagttttg gtgatggagc
    12961 atcaaatgaa ggtacttttc atgaaggaat aaatttatct tcaatatgga aattgccaat
    13021 aatattttac tgtgagaata atttatacgg tatgtcaaca agcataaaac gtcatatgaa
    13081 tatagaaagt attgccacta gagcagcttc ttatggaata gaaggaatat caatagatgg
    13141 atataaccca atagaagtat acgaaacagt acaaaaagct gctgagaagt gtagaagagg
    13201 agaaggacct gtattaattg aaagtagaac ttatagatgg ttaggtcatt caaaatcaga
    13261 tgcaaatgta tatagaacaa aagaagaaat agaatcatgg aaagcaaaag accctattga
    13321 atttttaaaa aattatctta tagaaaataa tctatctaat gaagatgaat tagataagat
    13381 tcaagaattt gcaaagcaat caatagaaga tgcagtagag tttgctcaaa atagcccaaa
    13441 tccaaaaata gagtctttat tagaggatgt atatgcagac taagaatggt aaaggaggaa
    13501 aataaaaatg agtacaaggg aattaacata tgcacaagca ataaaggaag caatgtcaga
    13561 agaaatgaga agagatgaaa atgtaatatt tatgggagaa gatataggaa tatatggagg
    13621 agcatttggt gtatcagttg gtatgataga tgaatttgga ccagaaagag taagggatac
    13681 tcctatatca gaagcagcta tagcaggagc ggcagcaggt gcagcagcaa ctggtttaag
    13741 acctattatg gaagtaatgt ttatggactt tgtaacaatt tctatggatg caattgtaaa
    13801 tcaagcggca aaaatgagat atatgtttgg aggaaaagca caagttccaa tggttgttag
    13861 atgtccagga ggttctggaa ctggttcagc agagcaacat tcacaaagtt tagaagcttg
    13921 gttttgtcat gtaccaggag taaaagtagt tgcaccttct acaccagcag atgctaaggg
    13981 attgttgaaa gcagctataa gagataataa tccagtcata tttgttgaga ataaattatt
    14041 atacagaaaa aaaggttttg tgccagaaga tgattatgta attgaaattg gaaaggcaga
    14101 tattaaaaga gagggtactg atgttacagt aattacttat ggaagaatgt tacaaagtgt
    14161 tgaagaagca gcagagactc tatcaaaaga aaatataaat gttgaaatta tagatttaag
    14221 aactttatat ccattagata aagagactat agtaaagagt gtatgtaaaa ctgggagagt
    14281 tttaatttgc catgaagcag ctaagactgg tgggctaggt ggagaaatat cagccctgat
    14341 aacagaaagt gaatcatttg attatttaga tgctccagta aaaagaattt gtggaaaaga
    14401 tgtgccaata ccatataatc cagagttaga aaaagcagtt gtcccaagag ttgatgaaat
    14461 agaagaagca ataaaatctt taattgttag ataaggaggg atagatatgg tggcaaataa
    14521 aataaaagcc acaccagcag ctagaagtca ggcaagaaaa gacaatataa aattagatag
    14581 gctgattgga agtggagaaa atggaaggat tcatttagta gatgtgttaa attatttaaa
    14641 agataataaa gcaaatacta ctcctttagc tagaagaata gcagaagatt taaatataga
    14701 tttagaaact attgtaggta ctggatataa tggaaagata agaaagtgtg atgtggaaaa
    14761 attaactgca aaagaaacta ttgtatcaac taatacatct aaatctagtg agaaaaaaga
    14821 gctgaaaatt gaaaatgaaa attcaagcat gtttaacaca gtagaaggaa tatttgaaaa
    14881 gccaaatcca atgagagcca ctgtagcaaa acgaatgtca gaaagttatt ttagtgctcc
    14941 tgtatttaca ttcaatatag aagtagatgc tacagaattg aaggtactta gagcaaagtt
    15001 aatcgacaca gtaaaagaat caactggagt aaaattaact atgacagatt taatagtaat
    15061 ggcagtatct aaaatacttc caaatcatca agccttaaat tcagcatgga cagatgaggg
    15121 tatatttaga tataaagatg taaatatagc tattgcagtt ggtttagatg aaggcttata
    15181 tgttccggta gtaaaaaatg caaacaaaaa gtctttaaaa gaaatagcaa aagaaagcaa
    15241 agaacttgcg gaaaaagtaa agacagggaa acttatgcca gcagaccaag aaggtaatac
    15301 atttacaata agtaatgttg gaatgtatgg tataactaca tttacaccta taataaatat
    15361 gccttcaagt gcaatcttag gggtaggtgc tacacaagat aagtttgttc cagtaaatgg
    15421 agaggctaaa ataaaaccta taatgaactt atcattaaca tcagaccata gagtaataga
    15481 tggaacagta gctgcaaaat tcttaaaaga tttaaaagaa ttgttggaaa atcctttatc
    15541 aatgcttgta taggttttga tattttgtta ctgatataaa tagtttgaat agattatatt
    15601 attataattg tttcagtaaa tttaaagctt ttatatatta agaattattt ttaacagtaa
    15661 ctatatgtga atatgatttg cttaattaaa atataagaaa ataaatagga ggagagccta
    15721 tgtcagtaga agtaataatg ccaaaagcag gagtagccat ggaagaaggt actatagtat
    15781 cttggctaaa gcaagaaggc gaagaagtaa agattggaga acctatacta gaaataacaa
    15841 cagataaagt taatatggag atagagtcag aaggagaagg tacacttgct gtaatcatac
    15901 ataaagagga aggtgaagta ttaccagtat ttacagtaat aggtgtaata gcagaaaaag
    15961 gggaaaatca agaggaagta aaagctaaat atttatctgg aaatatttca aaagaagata
    16021 cagttaaaga gaatcaaaat atagaagtta aagaagaaaa aataaacaaa aaagagtgta
    16081 atcatgatta tgatgtagtg gtaataggag gaggacctgg gggatattta tcagcattaa
    16141 aagcagctct tttaggtgga agagttgctt tagttgaaga aaatatacta ggaggtactt
    16201 gcttaaatag aggttgtatc cctacaaaga cttatataaa aacagcagaa atattagaag
    16261 aagttgacca attatcaaag agaggtgtaa aagtaactgt agataaagaa caggacatta
    16321 agaaggccat aaaatataaa aatagagtag ttaaaaaact tacagctggt gttggcggat
    16381 tattaaaaag tagagatgta gatgtgttta atcttaaagc tagtgttaaa gaagagcata
    16441 aagtaatttt atcagatgga aaagttttag atacagaaaa tataataatt gctacaggtt
    16501 ccaaagtaag gatattacct ataaaaggaa tagaatcaaa tttaataata actagtacag
    16561 aagcattaga tttggaaaca gtgccagaag aattagtaat aattggtggt ggagtgattg
    16621 gttgtgaatt tgcagagata ttcaattcaa gaggttctaa agtaactata gtagaaatgg
    16681 aagatagagt aatcccaaga atggataaag agttaagcga atctttaaaa tattctctca
    16741 gtaaaaaagg tataaatgta ttaactaaaa agaaagtttc tgaatttaaa gaagaaggaa
    16801 ataacatatt agtgtgcatt gaaggtgaag agccaataaa agcagattta tgtctatatg
    16861 ctataggaag agaagctaat ctttctggca tagaggaatt agatattaaa atagataaag
    16921 gttcaatagt tgtaaatagt aaaatggaaa caagtatacc aagcatatat gcagtaggtg
    16981 atgttactgg aggtgtaatg ttagctcatg ctgcttttaa aatgggagag gtagcagctt
    17041 caaatgctct aggtgtgaat aaagaagttg acttaggtgc attacctagt tgtgtttata
    17101 caattccaga aatagcatca gttggtatta cagaagagga tgctagaaaa aaatataatg
    17161 taaaagttgg taagtttaat tttgctggaa atggaagagc tttagcttct ggtcaagaac
    17221 aaggatatgt gaaggtagta gcagatgcta aatatggaga aattttaggt atacatatgt
    17281 ttggatgtgg tgtagcagaa cttataaatc atgcagcatc atttaaagcg ttagagatac
    17341 ctactgatga agcaagtgag ttgatatttg gtcatccatg tacttcagaa gctttaatgg
    17401 aagctttggc agatgtaaat ggagaatgtt tacacttacc taaaaaataa atctaaacat
    17461 aaattattaa tttaagtcta ttattaatat taatttatat cataaaattt taaaacactt
    17521 acttagccat accccttatt atgcaaataa tttatttgca taataagggg tattttttat
    17581 ggaaaaatat agaatataga atccttgtag tttttgttgt acaaaaaaga tagtaatata
    17641 aaataacatt atcattttag aatg
//
LOCUS       DAEMHZ010000080        17516 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000080 DAEMHZ010000000
VERSION     DAEMHZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.080"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..587)
                     /locus_tag="KPK72_002434"
     CDS             complement(<1..587)
                     /locus_tag="KPK72_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG2489641.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEQPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLSTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTS"
     gene            complement(599..979)
                     /locus_tag="KPK72_002435"
     CDS             complement(599..979)
                     /locus_tag="KPK72_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489642.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTALDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSTGSYTYTLSEDPVGIVFSTVDVTYGPQPVASMVEGYVITE
                     RLQGEYVKEAIDTIKTKLPNIKFM"
     gene            complement(995..1567)
                     /locus_tag="KPK72_002436"
     CDS             complement(995..1567)
                     /locus_tag="KPK72_002436"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2489643.1"
                     /translation="MKKGELIALGLSEEDAKKVEVESLKELDNYINKIEYEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQNKLKEKEQREFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDNWATQKSFLIKTGETKTNYTPDNGN
                     KNTLSRAENIAKEKNEEGSKNPYADAWSIK"
     gene            complement(1613..1843)
                     /locus_tag="KPK72_002437"
     CDS             complement(1613..1843)
                     /locus_tag="KPK72_002437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489644.1"
                     /translation="MKEYVIWFKSGNCVSGITNEDVTDKLMKDFMEADSDCRYLKGYL
                     DEDGTTIIDLSQIEAISINNCNENNNIGFSKS"
     gene            complement(1894..2166)
                     /locus_tag="KPK72_002438"
     CDS             complement(1894..2166)
                     /locus_tag="KPK72_002438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489645.1"
                     /translation="MFKQKYIKKPNKISARNIIAFIITVIGIALGVFIGINIIMAHVL
                     GIANMIDNSTFTCVRLVYGLVGVVSGYLIGKAIYLIASLISYIIYE"
     gene            complement(2186..2683)
                     /locus_tag="KPK72_002439"
     CDS             complement(2186..2683)
                     /locus_tag="KPK72_002439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489646.1"
                     /translation="MAKFVKKPVEVEAFRFDIDVEPTVWIRKEIERGNVWVEHSIDKE
                     HTKMFIRTLEGLIYQVNIGDYVIQGVKGEIYPCKADIFEMIYEKVEYRGKNKLSTKMT
                     LDSTNFQENIKNAAKELEFFIETLEKADDKIDKLTKKMNKCACKVDTDKIVKQLVEHL
                     REGIE"
     gene            complement(2996..4018)
                     /locus_tag="KPK72_002440"
                     /pseudo
     CDS             complement(2996..4018)
                     /locus_tag="KPK72_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(4008..5411)
                     /locus_tag="KPK72_002441"
     CDS             complement(4008..5411)
                     /locus_tag="KPK72_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2489647.1"
                     /translation="MELDVIKKLIEQTNSKHSNFVKKADEAEKYYKNQNDIIRDRSPN
                     NVGKVNTANNPLRNADNRIPFNWFGFLVNQKISYLFTYPPTFDVGDDGINSKITDILG
                     DRYPKEAKTLGKNASICSKAWLHVWEDDNNDFQYTNIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDKGKEYAIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NIYKHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRMICNHIGYSPKR
                     VLQTWTRNMIQNDLELADICSKSVGIVSEKTNLKNHPLVDNAEEEEKQIKKEKEDSNQ
                     EYNNVIPNNQDGVIDET"
     gene            complement(5416..6684)
                     /locus_tag="KPK72_002442"
     CDS             complement(5416..6684)
                     /locus_tag="KPK72_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2489648.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGVRILTPNGRR"
     gene            complement(6662..7345)
                     /locus_tag="KPK72_002443"
     CDS             complement(6662..7345)
                     /locus_tag="KPK72_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489649.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWVSE
                     KKKGAPKNKKGAPFGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKTEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            complement(7408..8085)
                     /locus_tag="KPK72_002444"
     CDS             complement(7408..8085)
                     /locus_tag="KPK72_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBG2489650.1"
                     /translation="MLKILQEKNVKVMWSKNGEDVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVS
                     KALKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKDVKRDELNQSELFDIQALERVISMKLPKLIDEDMNYKEVYKKI
                     KELIEMI"
     gene            complement(8332..8544)
                     /locus_tag="KPK72_002445"
     CDS             complement(8332..8544)
                     /locus_tag="KPK72_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489651.1"
                     /translation="MSNLREKIIKELDEFNIDADDEFLDYGVEYVESFIGKSAISEEL
                     LIRGVVLTANYIVNMKAKDSVNKGLD"
     gene            complement(8849..9970)
                     /locus_tag="KPK72_002446"
     CDS             complement(8849..9970)
                     /locus_tag="KPK72_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2489652.1"
                     /translation="MNNFNGERLNKARIYREMTVFELAQKMNCERQLISMYENNKLKP
                     EKNIIKQIAKELYFPVKFFLEKENDIIKGSSYFRALLTTNKKYRKKQIQRMEFLAQIY
                     FFLQDYIEFPKLDIPNCFYKTPEESVLLLREAWGLGLKPIDNIIYEVEQHGIIVTGFP
                     TSIDYIDTFSQMIDIEGKTMYLIGYSNNNTSTSRLHFDIAHELGHICLHEWSEDVEAL
                     EKQEFKDRESEANRFASTFLLPEETFKIDAKRTPLRIPNYTELKRKWKVSIQAMIRRS
                     YSLGIISMDEYQSMIRTLQRRGLRKSEPLDDELLTSLPALLKTAVLMLLNEKVFTPKE
                     LICNFSLESKELENILDLPNNTLNSSKILYLDVNRLGRA"
     gene            complement(10136..10642)
                     /locus_tag="KPK72_002447"
     CDS             complement(10136..10642)
                     /locus_tag="KPK72_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489653.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSANIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNVIEDAVVNISDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHKQIITYKYIEGLEWSLIVDKVYLEERQLRERANQAINSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            complement(10748..11596)
                     /locus_tag="KPK72_002448"
     CDS             complement(10748..11596)
                     /locus_tag="KPK72_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG2489654.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHDIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIESVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSRAYK
                     NKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            complement(11623..12354)
                     /locus_tag="KPK72_002449"
     CDS             complement(11623..12354)
                     /locus_tag="KPK72_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBG2489655.1"
                     /translation="MNDLKLIENEGIIKVYTTDEDIKVVDGRELWEGLGVKQDFSDWI
                     KTNLKSVDAIENKDYFTIPFKRERQILIEYTLKLEIAKEICLVAGASPRANKELKRNS
                     KNYRKYLIAVEEKYKASNNLTKTQLNQINDIVSNALYEMQTKHDAQIEQFKRESSQYY
                     RPTSKTKYDISSYIKDRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSLNII
                     DESIRIIKSERKTNQISFFEKDNFC"
     gene            complement(12417..12824)
                     /locus_tag="KPK72_002450"
     CDS             complement(12417..12824)
                     /locus_tag="KPK72_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2489656.1"
                     /translation="MKINFTIDGEPVGKERPRMNSITKRTYTPNKTKNYEELIKWLYQ
                     SKVRYYFDGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIVADALN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDIS"
     gene            complement(12814..13218)
                     /locus_tag="KPK72_002451"
     CDS             complement(12814..13218)
                     /locus_tag="KPK72_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489657.1"
                     /translation="MQNKKDVWLYGWNNKYLSSDEYESKDEAIQAVKEELKEFGNFAE
                     SIYVGRKEKVNIPNIDVEEALEHIQEKIDDEVGECGEDWFENIRVEDILILENRISEV
                     FKKWIDEFGYKPYWFVVRDTEEIELNEVANED"
     gene            complement(13232..13447)
                     /locus_tag="KPK72_002452"
     CDS             complement(13232..13447)
                     /locus_tag="KPK72_002452"
                     /inference="COORDINATES: protein motif:HMM:NF024363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBG2489658.1"
                     /translation="MIHELQIASSTFKDVIKNKKQIEVEHTVDFKAGDTVILQSFKDK
                     KFTGIEVNKEIEYFVKLGNGYVELGIK"
     gene            complement(13544..13873)
                     /locus_tag="KPK72_002453"
     CDS             complement(13544..13873)
                     /locus_tag="KPK72_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489659.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNNTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            complement(13955..14953)
                     /locus_tag="KPK72_002454"
     CDS             complement(13955..14953)
                     /locus_tag="KPK72_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2489660.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYTEKDEKKVAILKLDYNKSYTHSISFEDDKFNIQMSKNEINIQETKAIKIGAIIG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKVFKALVDAFIENLY
                     SDMKQGEDVRSILLYMLREKQKLDINEFTEIAIKEDLKDSFKDHAEEKGIDGSFNIDK
                     KWVEKNLKNRCIKTDTGFEIKGKMDDFEDFMKYGIRHNGNGTVDIVIKNVSFYNEK"
     gene            complement(14967..15158)
                     /locus_tag="KPK72_002455"
     CDS             complement(14967..15158)
                     /locus_tag="KPK72_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489661.1"
                     /translation="MLICVMVKTNKKGSYTHGEIKVDDDDLKGMSDEEKTEYMKKIAQ
                     EYICENLIDWYWFGIGENN"
     gene            complement(15325..15507)
                     /locus_tag="KPK72_002456"
     CDS             complement(15325..15507)
                     /locus_tag="KPK72_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489662.1"
                     /translation="MTNFEMIKNMSKKELAEFINEIGDNCTCIYCSCQYRYEICDVDE
                     KCVDGCKEWLDMEVEL"
     gene            complement(15500..15658)
                     /locus_tag="KPK72_002457"
     CDS             complement(15500..15658)
                     /locus_tag="KPK72_002457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489663.1"
                     /translation="MDDRLEICKKMFPNVTKNTRLLCHYCDGRNICSYDEEKARKLLR
                     EKEEVKND"
     gene            complement(15774..15950)
                     /locus_tag="KPK72_002458"
     CDS             complement(15774..15950)
                     /locus_tag="KPK72_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019279463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG2489664.1"
                     /translation="MKLKDIIKLGEKYCYCPNCGNDKIGNNEGKLIVEEHTYYRECSC
                     GFSILIDDRKDEIQ"
     gene            complement(15947..16105)
                     /locus_tag="KPK72_002459"
     CDS             complement(15947..16105)
                     /locus_tag="KPK72_002459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489665.1"
                     /translation="MKVQMQLTKDKEFFKVCVNSEEEEMERMFYEFVSQMLACKRKNK
                     KVQGDIEK"
     gene            complement(16080..16442)
                     /locus_tag="KPK72_002460"
     CDS             complement(16080..16442)
                     /locus_tag="KPK72_002460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489666.1"
                     /translation="MLKEDCEWQKVSNVGVFSKCWSGNNEEIYEGDILKEPYNTKNKY
                     EYGIVRQENYALGLYVEWHYLKQFEDKWEELTSKAIIMNSKEYIVVGNEYENLEEVRK
                     EFLESKEKLENESTNAVN"
     gene            complement(16454..16804)
                     /locus_tag="KPK72_002461"
     CDS             complement(16454..16804)
                     /locus_tag="KPK72_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489667.1"
                     /translation="MAKIWMDAGTFLERTKDIGDIECNLREMINKNKQKNLIGRINSK
                     GQGRKSKKVECTNIITGESKIFVSAVEASKYLYFTELHITRLARTGKTTKNGWKVRYI
                     QEVTDGISKCGAML"
     gene            complement(16821..17072)
                     /locus_tag="KPK72_002462"
     CDS             complement(16821..17072)
                     /locus_tag="KPK72_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489668.1"
                     /translation="MIIIRSQDRLDLMRVNRVEISNNRVYAMFEDGIRKMGEYESNER
                     AIQVLNEIQEFIENGVRTDYIDSCRVRHNQEKVFEMPVE"
     gene            complement(17151..>17516)
                     /locus_tag="KPK72_002463"
     CDS             complement(17151..>17516)
                     /locus_tag="KPK72_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2489669.1"
                     /translation="ELKYLPNSGTPKITFSMAVDRRFKDKNGNKITDFIQCEQLGKHV
                     ENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALELLSSKSDSNPKQEYI
                     PPGLNPQGFQAIDDDDIPF"
BASE COUNT         5160 a         3379 c         1714 g         7263 t
ORIGIN      
        1 gaggttaatg accttgttgg agtaaatcca gtatcaacaa ctattttatc agtcatatta
       61 taaatatctc ctaatatatc tggtgttcca ctagaccaag tttttgtttc tttatgattc
      121 gttggagttc catattttaa agaagcttta actccatttt catttatatt caattctcca
      181 gttgataaaa cttccattct cattgcttct attctagtat ttacacttga tacaaggtta
      241 tcaacatcat taaatatttg atttatcatt tgagtttctt cttgagaatt tcttggttgt
      301 tcaagtacaa ttatatcttt ttcatctagt ttaatttttc ttttcacaag tgcaagttca
      361 gcaatactta aattagcacc ttctcttgat gcaatctctg cttctgtatc aaaagcatga
      421 acacttgctg atactggaag attagatgca ccttttatca tctttatttc aagtccttct
      481 atcttttgag ttggaaataa taaatctccc attgtttctt ttaattttct agtctttgta
      541 tagtttatca gctcttgaac tgacaataat tcttctactc ttgccatatt tcatcctcct
      601 acataaattt aatatttggt aatttcgtct ttatagtgtc tatagcttct tttacatact
      661 caccttgcaa tctttcagtt attacatacc cttccaccat tgatgcaact ggttgtggtc
      721 cataagtaac atctacagtt gagaaaacta ttcctacagg gtcttctgat aatgtgtatg
      781 tataactacc cgtagaacct cctctagtta tctttaccac tttgccactc tcacctaata
      841 aactacctgc taacacatat ttctttccat tttcatcagc cactacatct gtatctaatg
      901 ctgtttttga aaagttaata tagtgttgag aagctagaaa ctcgggtgtg ttatcaaaat
      961 tcacctcttt aaaatacata ctttatcctc ctttttattt tatactccat gcgtcagcat
     1021 atggattttt agaaccttcc tcattctttt ctttagcaat attttcagcc ctgcttaatg
     1081 tatttttatt gccattgtca ggagtgtaat ttgtcttagt ctctcctgtt tttattaaga
     1141 aagacttctg agttgcccaa ttatcagtct gttcctttag ccctgtaaaa gtaccatttt
     1201 catattttat tttttctaaa tcaagttctg cttttgctgc tttagtgcta tgaacattaa
     1261 gtttaataag ttcattttct aacgccatat caaactctct ttgttctttt tcctttaatt
     1321 tattttggta ttcttcatcc tttgcagtta atttagtctc atatcctttt ttaagctctt
     1381 caatttgctc ttttgtcatt ccatccttga aaccttcaat ggcttctttg gatgctttta
     1441 attcttcttt taccttttca tactcaatct tattaatgta attgtctaat tcttttagcg
     1501 attcaacttc tactttcttt gcatcttctt cactaagacc tagtgcaatt agttcacctt
     1561 ttttcatttt atttaattct cctttcattt ttgcaaaata aaaaagcctt atctaagact
     1621 tactaaaacc aatattatta ttctcattac aattatttat tgatatagct tctatttgtg
     1681 ataaatctat tattgttgtt ccatcttcat ctaaatatcc tttcaaatac ctacaatctg
     1741 agtcagcttc cataaaatct ttcattaact tatcagtaac atcttcgttt gttattccag
     1801 atacacaatt tccactctta aaccaaatta catactcttt caataataca accctccttt
     1861 catttcttac aaaataaaaa atctttgaac aaattattca taaataatat agcttattag
     1921 tgaagctata agatatattg cttttcctat taaatatcca cttacaactc ctactaagcc
     1981 ataaactaat ctaacacatg taaaagtact attatctatc atattagcta tacctaaaac
     2041 atgagccata attatgttga tacctataaa aacacctaaa gctataccaa taactgtaat
     2101 aataaatgct ataatatttc tagcacttat tttatttggc ttcttaatat atttctgttt
     2161 aaacatattt ccatcccccc aaaacttatt caataccttc tcttaggtgt tccactaact
     2221 gttttacaat cttatctgta tcaactttac aagcacattt attcattttt tttgttagtt
     2281 tatcaatttt atcatctgct ttttctaaag tttctataaa aaattctaat tcttttgcag
     2341 catttttaat attttcttga aagtttgttg aatctaaagt catctttgtt gataatttat
     2401 tttttcctct atattcaact ttctcataaa tcatttcaaa tatatctgct ttacaaggat
     2461 atatttctcc ttttacacct tgtataacgt aatcccctat gttaacttga tatattagcc
     2521 cttcaagagt tcttatgaac attttagtat gttccttatc aatagagtgt tcaacccaaa
     2581 cattgccacg ttcaatctct tttcgtatcc atactgttgg ttctacatct atgtcaaatc
     2641 taaaagcttc tacttcaact ggtttcttca caaatttagc catattactt atccctcctt
     2701 aaagttttaa tcacatcttc attactaata agcaaagaat atagtatgaa tatcacccca
     2761 agaataaaat taagtagtgg aaataaagcc ataaaaataa aattacactt tcttcttact
     2821 tttctatttt taagaatctc aatcaattcc tcattactat caatcttcat tttaaataaa
     2881 taaagccctg tacaaaacac aattattgat aaaatatata atttaagcat ttcaaatcac
     2941 cctctcaata aatttttaca taataaaatc acctactaat ttagtaagtg tctctaatgt
     3001 atttcttata ccattcatta tacttcatac tagatggtac ataatatgtt tttccatcta
     3061 cttcttttgc tgctctgtag ccttcttcat cactaaaaaa aggagctgtt gttgttctgc
     3121 aacgacaatg aaatggtgga gctgttattc caacttgata gtcttttata tcaaatactt
     3181 ttccatctaa ctctctgcat atatttgaag ttcttaaatc taatgtggca ataatctcat
     3241 atttctctac atctaaatca ttaaaacaat cttttcttga agctgatgca aagaaagctg
     3301 attcagtcat tattaaattc ttagcttgtg acttagatac attaaatctc ttagcaaagt
     3361 catttactag atttttagga ttttcacctc taataattga ttgtgtcagt ttagtatgta
     3421 gttcatttac taaagttggt ctatgctttc cccaaatcct ttcactaaag tttaatccat
     3481 cactagtcca aggttttgag ataattttat ttattctgtt agtgtcaaga ctcattaaac
     3541 tccaaacaac accaatacct tgttgaacat taaaagctgt atgataatat ccacttgtat
     3601 aaatatctct cattagttta tcaatgctat caagttcatt tccataaaga acttccactt
     3661 gttgctgtat ttgcagtttt aaagcttcaa gccttgttat atgaactcta gcactagcat
     3721 tttctaactc tttcatccac ttttgactcg tagcattttc tttaccatac ttaatatatt
     3781 cttctacact ccatttaaac tcatctagtt ctcttgtatt tagtagtttc ttagcttcta
     3841 ataaagatat tccttcattt ttggcaaatc tgttgtacca tgacaatata tctttttcta
     3901 tactattcat agctaattta tattgctttt ctaattcaag atagtatttt atacttttat
     3961 tattctgagc ttcttctaat tgttcaaatc tcttcttcca ataatcttta tgtttcatct
     4021 ataacaccat cttgattatt aggaattaca ttattgtatt cttgattgct atcttccttt
     4081 tcctttttaa tctgtttttc ttcttcttct gcattatcta caagaggatg attttttaaa
     4141 ttagtctttt ctgatactat cccaacactc ttagaacata tatcagccag ttctaaatca
     4201 ttttgaatca tattcctagt ccaagtttgt aaaactcttt taggcgaata tcctatatga
     4261 ttacatatca ttcttactag tttagcaaac cctaatctaa actctgtttc cataagtcct
     4321 gcttttaatt ctaacaaagt gtataagaat ttgagtgcta caccacttgt attggcaaaa
     4381 ttttcgggtt tagggtcaac gccttgacct tgtacatata tttgcttttc agttgtttta
     4441 agaagtgaat ctcgagcttc aattggaata tttattgtaa ttgtacttaa cccactctta
     4501 tcatctgcac cactactttg taaatcaata gttttatatt ctttaagtcc ttttaaaaac
     4561 tctgttaagt ctgcacctcc ataattcgta agaacgaaaa taacttcttg tatatcttca
     4621 atatcattca caaaaccgct ataaaccttg tcgtatacat caatgaggtg tttaacatta
     4681 tctaaatctc ttacctctaa atcattgttt agaaattcga taaatggaac ttctccaaaa
     4741 ttgtgtttat atatattagt ttgagtttca agtttattat ctaaattttt ctctataaat
     4801 ttattaagta tctccaatcc atttatatta ctatttccat ctttattttg ataagtataa
     4861 caacattcat ctgtccaata ctcataaatt gcatattctt ttcctttatc atcagtcttt
     4921 ttatattctc taagtactgc aagaagtttt ctgtttaaat ctgatgaata tactgctctt
     4981 atttggcgag ggtctatatt agtatattga aagtcgttgt tatcatcctc ccaaacatgt
     5041 aaccatgctt tagagcatat actagcattt ttaccaagcg tcttagcttc ttttgggtat
     5101 ctatcaccta aaatatcagt tatttttgaa tttataccat catctccaac atcaaatgta
     5161 ggaggataag taaacagata tgatattttt tgattaacta aaaaaccaaa ccaattaaac
     5221 ggtattctat tgtctgcatt tcttagtgga ttattggctg tatttacttt gccaacatta
     5281 ttagggcttc tatctcttat aatgtcattt tgatttttat agtatttttc agcttcatca
     5341 gcttttttaa caaagttact atgtttacta ttagtttgtt caattaactt ttttattaca
     5401 tctaattcca atttatcacc ttcttccatt tggtgtaagt attctaaccc cattattttt
     5461 aagtttatta tctatagaat atctaagagc tgccattgca tcatccataa actctactgg
     5521 ttcatcaaga tataatccag ttctttcgtc ttgtttccac ttccactgtt gaatttcttt
     5581 tattgtatta gtgcaactag gatgtacatg tattcttaat tgtttcaaat aatctatttg
     5641 agctttaaca cttcctggtc cttttttaac tcctttagct ttatatcctg cactcttcca
     5701 catcttaatt ctatctggtt cagcactatc acagtacata aatagattct tttctaagcc
     5761 tttactattt gcaatcttta ttatttcaga ggtatccatc tcatgtacat atatttcatt
     5821 acatatatat aactctccat ccttaaaacc aattctaagt actacatttg catggttaaa
     5881 tccaaagtct tgcgataacc tcatattgtc aaaatactca aattctgtag gaaattcatg
     5941 tataacataa tttttaagta ttgctccacc agtttctccc cattctccaa gaccatatac
     6001 tttgtaccct tctgggtctt gctcttttct catttgcatt cttctgtagt aagcttcatc
     6061 tatgaatcta ttttgtagat aagtactatg atgagtaaat atatcatcat ttttatagtc
     6121 aaaatacttt ctttttatcc aatgagtagc tgagactgga ttaaatgtaa atgtcatttg
     6181 atagtatagg ttaggattag ttaaaatacc tcttaaacgg tcatctagta tgtctatgtc
     6241 actttccata agttctgtag cttcttcaca ccaaacccat gttaacttac cttttgagaa
     6301 gttaattgat tttaattttt ctctttgttt tgcatcatta acgcctctga agattataga
     6361 gttaccagta actttacttt tgatttctaa aggatttaaa gtagttttcc aatacttatc
     6421 agcttgttta ccataaatac gatttatagc tcctgtaagc tctgcatacg ttgaatactt
     6481 atgtgtggct tctgactttc taactactaa tagattagct ccttgatact tcttatctcc
     6541 taactttagt atatagtctt gtgctacatt aacagatttt ccactccctg ctgaaccttt
     6601 cattgctctg tatctttttt tagtaaaatt agcttccttg aaatctggat taaaatttat
     6661 tctaactatc atttctatca ccataatcta cacttatttt caactcatca tctccaatat
     6721 catctttact taggttatca acttcacatt tcaacttctc aactcttgtt ttctgctcct
     6781 ctgtagccaa attccaatcc ttatgaatca tttcatcata ctgtttaatt aaacttctaa
     6841 gttcactcat tgctctactc tgtgcattaa gaaaagatgc ttgcctatcc catgcaaatt
     6901 gaaattcata ctctgtcttc tctccatttt ctgtactttc atgtttcttt aattctttaa
     6961 tcatttcttc tttatcttta acatacatta tcttctgtgc tcttattatt gctgcatatt
     7021 ggattgttat ctgctcccaa agaatatcga atttatcttt attctttatc tcattaatta
     7081 ggtcttgagt ttcttcgggt aggtactttg agaagaagcc aaacttctca gcattcttat
     7141 taccaggtgg accagtagca tttttattac caaagggtgc acccttttta tttttaggtg
     7201 cacccttctt tttttcacta acccaattgt atcttttaat ccatgacttt aaagtgttta
     7261 aactaatgtc atactttgct gatatttcct tttgtttcat accttttagg taatcttgtt
     7321 ttaccttttc tttgacatct tgcacatcac cacctcgttt gtttgtcgtt ttgggaatta
     7381 aaaaagaccc tccatcaaga cagtccctta aatcatttct attaattcct taatcttttt
     7441 atatacctct ttataattca tgtcttcatc tattaactta ggcaacttca tagatatgac
     7501 tctttcaagt gcttgtatat caaacagttc actttgattt aactcatctc tttttacatc
     7561 ttttggtata cctaattttt ttcttacaag ttcagtaaag tgtttataat acatctgagg
     7621 tttattactt ccttgactag tagcataata aacaaattct tgtatttcat ctgtaaaatc
     7681 ttttctaacc tttttgcctt ctgttcttat atccagccat tcttggtctt tttctgtagc
     7741 aatatagtaa ccatgtattc taatttgttt aagtgcttta gaaacccatt ttgtaaatag
     7801 ttttgcttct gctttattgc ttctaaatga catattgtac acagcttctt ctgtaacaaa
     7861 agtagtaccg aagttaggca atttatcttt aaagtttcta gtgtaggaat ctccgacagt
     7921 agattcatta aatttctttt tatattctct atctatattt cttaatgtgt cacgaatatt
     7981 tactattcct aattcctctc ctacatcatt tgcattaaac caaacatctt ctccattttt
     8041 agaccacatt acttttacat ttttctcttg taaaattttc aacatactac tacctcctaa
     8101 attatttata ttaataaagg gagctcaatt ttaacatcct tttatatatc gattcctcaa
     8161 tttgagcccc cgtcatttca taaaatcaac cttctgggca tttgaatttt agatatgcat
     8221 aatatgcata tccgaatagt gcatattatg catttttaat agaccttctc tactgtcaga
     8281 gttaccgaca tctgaaattt attaaatttt tgtataaaaa agacctagaa attaatctaa
     8341 gcctttattt acactatctt ttgctttcat atttactata taatttgctg taagcactac
     8401 tcctcttatt aataactctt cgctaatagc acttttccct ataaaactct caacatattc
     8461 aactccataa tctaagaatt catcatcagc atctatatta aattcatcta attcttttat
     8521 tatcttttct cttaaattac tcatttatca tactcctgta aaaaaataaa agactaagct
     8581 tacccgaact tagtcttttt ataaaaaagg gggaggtata tatattatgt cgcaagttct
     8641 aagaatcgaa cttagattaa acaccagcac ctgcatggtg agtgaggtta ccaagcccca
     8701 ctcggttttt agacttctga attaagatac aaaactgtat gagattttaa tctcaattca
     8761 aatactattt tttagtgtat ctgtagatta aaacttcaag agaaaatctt gaagattgaa
     8821 catagttaga attgaactaa cagcgtcctc acgccctgcc tagtctgttc acatctaagt
     8881 acaaaatttt actagaattt aatgtattgt ttggtaaatc taatatattt tctagttctt
     8941 ttgactctaa actaaaatta cagataagct cttttggagt gaataccttc tcatttaata
     9001 acattaaaac tgctgttttt aacaatgctg gtagagaagt taataactca tcatctaatg
     9061 gttctgattt tcttagccct ctacgttgta aagtacgaat catagactga tattcatcca
     9121 tactgataat tcctaaagaa tatgaacgac gaatcattgc ttgtatagaa actttccatt
     9181 tacgtttcaa ttctgtatag tttgggatac gtaaaggagt tctcttagca tcaattttaa
     9241 aagtttcttc tggtaataag aatgtagatg caaagcgatt agcttctgat tctctatctt
     9301 taaactcttg tttttctaaa gcttctacat cctcactcca ctcatgcagg catatatgac
     9361 ctaattcatg agctatatca aaatgtagtc tagaagtaga agtgttattg ttggagtacc
     9421 caatcaaata catagttttt ccttcaatat ctatcatctg actaaatgta tctatataat
     9481 ctattgatgt aggaaatcct gtcacaatta ttccatgctg ttctacttca tatatgatat
     9541 tatcaatagg ctttaatcct aatccccaag cttctcttaa aagtaaaaca gattcttcgg
     9601 gtgtcttata aaaacagttt ggtatatcta attttggaaa ctcaatataa tcctgtaaga
     9661 aaaaatatat ttgagctaga aattccattc tctgaatttg cttttttcta tattttttat
     9721 ttgtagttaa taatgctcta aagtaagacg aaccttttat aatatcattt tctttttcta
     9781 aaaaaaattt aactggaaaa tataactctt ttgcaatttg cttaattata ttcttttcgg
     9841 gttttaattt attattttca tacatagaaa taagttgtct ttcacagttc attttttgag
     9901 ccaactcaaa tactgtcatt tctctatata tacgagcttt atttaaacgt tcgccattaa
     9961 aattattcat atttaatact ccattcaaaa cttttatatg ttggctagag tacagataat
    10021 cccaacccta gccaactatt taattttgag agggaaattt ttcatttcca caatactatt
    10081 atctcatgtc taaaattaaa aaaacggcac attttcggca attaaaatct ataacctaat
    10141 ttagtttgta atctaacaat tcaaaaagag gttcttgttc tattaatgct ttcttcccaa
    10201 acaatgctat tgatattgaa tttatagctt gattagctct ctctcttagt tgtctttctt
    10261 ctaaatacac cttatcaact atcaaactcc attctaagcc ttcaatatat ttataagtta
    10321 taatttgctt gtgtataggt tttaaattcc ttatagctaa atctattgta gatttgatta
    10381 tttctgcttc atatagctct atttcttttt ctgttattaa gtcagatata ttaacaacag
    10441 catcctcaat cacgttacta gttttgtttg tttttcctgt tttgacacta tcataactta
    10501 ttcctttcat taaatctcca accgaattat cttttaacat ttgtatttca tttttcagtt
    10561 ttattatatt tgcacttaat tgtttataat tagaaagttg tttcttagtt gcattaaaaa
    10621 actctttttt agttttagac atactcacac actcctatca attatgttat aataatgttg
    10681 tgatgacagt tttagatttt tgacaactgg agtgtgagag cactcctttt cctttttttg
    10741 aataaattta actcactatt gataattgac tatttaaaag tcttatttct tcctcaaaca
    10801 ctataggtaa cttataattg tttacaatct ctaacacttt atctaattgg caacgcttta
    10861 tagccttata actatctact ccaaactctc gtttaatctg atgatatata tcactgtata
    10921 ctttacctct taaagattta tttttataag ccctacttcc atgacctcct agtgattttg
    10981 ttgctacctt cttaacttct tttacaatac tttcacattc aatattgaat agtggtgcat
    11041 catccataaa gttctctaat ttctcattaa cactctctat tttagtttct aagacttctt
    11101 gtttcttatc tagcataaat atagcttgta actcctttga tgcacttaaa agaggattat
    11161 ttagttcttt tctcatagag aaatatccat caactaactt ctcatataat tcccaagcta
    11221 tatcatcttc taatattttt aataattttg cataacctct ttcagataat atataaatcc
    11281 cagataataa ccctttgttt tttaaccctc tataagaatt aattgattgt tgagtaaatc
    11341 ctaattcttt tattttggta tcgtccaaac cgacacctaa caaatctaat atatcttttc
    11401 catctttaaa tctttttcta ttcttattta taagctcatt aatctgtcta gattctctat
    11461 tatgtatctc ggctatatct tttactagca ttgctttctt atgttctcca aatccaccct
    11521 caatatcatg aaatttcatt ccctcgattt ctaaagttcc aagtactgtt atttctttat
    11581 ttatattttc attcataatt tatctctcct tttctctttt aattaacaaa aattatcttt
    11641 ttcaaagaaa ctaatttgat ttgtttttct ttcggatttt ataattctga ttgattcatc
    11701 tataatattt aatgatttta ataaaacatc ttttggtata tcctcccact tatcagctcc
    11761 aagaattaat aaagttctct ttttaaccaa atcaaactct tcattagctc taggaatatc
    11821 taatctatct tttatgtagc ttgaaatatc atacttagtt ttacttgtag gtctataata
    11881 ttgtgaggat tcccttttaa attgttctat ttgtgcatca tgtttggttt gcatttcata
    11941 taatgcgtta cttacaatat cattaatttg atttaactgt gtttttgtaa gattgtttga
    12001 tgctttgtat ttttcttcta ctgcaattag atactttcta taatttttag aatttctttt
    12061 aagttcttta tttgctcttg ggcttgctcc tgctaccaaa caaatttctt ttgctatttc
    12121 taatttcaaa gtatactcta ttagaatctg acgctccctt ttaaaaggga ttgtgaaata
    12181 atctttattt tcaatggcat caacactttt taggtttgtt tttatccaat ctgaaaaatc
    12241 ctgtttaaca cctaatcctt cccaaagttc cctaccatct acaaccttta tatcttcatc
    12301 tgttgtatat acttttataa tcccttcatt ttcgattaat tttaaatcat tcatacttat
    12361 aatcctcctc caaattattt ttatctaatg atttgataaa cggatttttc cgttgttcaa
    12421 ctaatatctt ccaatataac ctcaactctt ggtttatcac tgtaatattt actagctaca
    12481 acctcaacaa tctgtgtatc atccttataa gctatctcat taagtgcatc agcaacaatc
    12541 ttaacaacat tatcaatatc gggtttcttg ctaggtctta acacattatt tcttttctgc
    12601 tctttaactt ttttactgtt acttttagct atagagtaat agcatcttaa agtcattttt
    12661 atgtatccat caaaatagta tctaacttta gattgatata gccattttat taattcctca
    12721 taattcttag ttttattagg tgtataggtc cttttagtta tagagttcat tctaggtctt
    12781 tctttgccaa ctggttctcc atctattgta aaattaatct tcatttgcga cctcatttag
    12841 ttctatttct tccgtatctc taacaacaaa ccaatatggt ttatatccaa attcatctat
    12901 ccatttttta aatacttcgc ttatcctatt ctctagtatc aatatatctt caacacgtat
    12961 attttcaaac caatcttctc cacactctcc aacttcatca tcaatctttt cttgaatatg
    13021 ttctaatgcc tcttctacat ctatattagg tatattaact ttctcttttc tcccaacata
    13081 aatactttct gcaaaatttc caaactcttt tagttcttcc ttaactgctt gaatagcttc
    13141 atctttactt tcatactcat cactacttaa atatttgtta ttccaaccat atagccacac
    13201 atctttttta ttttgcatat aaattcctcc tctattttat tcctaactca acataaccat
    13261 ttccaagctt aacaaaatac tcaatctcct tgtttacttc aattcctgta aattttttat
    13321 ctttaaatga ctgtaatata actgtatctc ctgctttaaa atctactgta tgctctactt
    13381 caatttgttt tttattctta attacatctt taaaagtact agaagctatc tgcaattcat
    13441 gtatcataca atcacctctt tataagtcaa agcaagtcta taacattcta gtttcattca
    13501 caaacttacc ttgactattt atactccaac caatttttat tatttaatat ttaggaaatt
    13561 ctccataagt cgtagcaaga cattcaattc tccactctgg tataacccac gttgtattgt
    13621 tatcacccac atatgctttt actttataaa gcggagtatt tctttttcta aatttcgtac
    13681 tataacattt ttctaaaatt tcacaaccaa ccattctgtt atagtaacag tttatttgat
    13741 gatatataat cttttcccct acagaatatt tatattttaa attatttata gattttttat
    13801 atatgcgatt tttcacataa tttgctaata tacaaataat tattagcagt atagtcaata
    13861 aaaaaatctt cataagttat tcctcctaac tagtttaaat ttacatcctc ttattattta
    13921 cttctacttc tctagccaat agtcacatga gtacctactt ttcattataa aaacttacat
    13981 ttttaataac tatatctaca gttccattcc cattatgtct aatgccatat ttcataaaat
    14041 cctcaaaatc atccatcttg ccttttatct caaaacctgt atctgtcttg atacatctgt
    14101 ttttcaaatt cttttcaacc cattttttat caatattaaa acttccatca ataccctttt
    14161 cttctgcatg gtccttaaaa ctatctttta aatcttcctt tattgctatt tcagtaaact
    14221 catttatatc aagtttttgc ttttctctta gcatgtaaag caaaatgctt cttacgtctt
    14281 cgccctgctt catatcacta tataaattct ctataaaagc atctaccaaa gctttaaata
    14341 ctttagtctt atacttatca tcttttatct tagtagcatt taaaaactct gtaacaaact
    14401 tagaattagc ttcttccttc tctgcatcct tatccaatac ttttagatga tattcgtcat
    14461 tcattccact caaaccaata attgccccta ttttaattgc tttagtctct tgtatattaa
    14521 tttcattctt agacatttgt atattaaatt tatcatcttc aaagctaata gaatgagtat
    14581 acgacttgtt gtaatcaagt tttaatatag caactttctt ttcatctttt tcagtatata
    14641 aacatattgc taagtcgcaa gattctaatg tagcattcaa tttcataaca tcaaataaat
    14701 aagctgcaat ctctttagag ttatttaaaa atgaactttc atcataaata atttgttcgc
    14761 aacacttctt aattagattg ttactatagt cattaaatac tgctgttctg atatcattat
    14821 ctcttgatac tttgcttatt ttcttttgaa aaaataggtc catatcttga ctgactctac
    14881 cttcaaaatc gttcaatatt ggtgtatcgc tattcttatc taaaacatgt attataaatt
    14941 tgtgtattat cataattcca ccccttttaa ttattttctc caattccaaa ccagtaccaa
    15001 tctataagat tttcacagat gtattcttga gctatttttt tcatatattc tgtcttttct
    15061 tcatcactca taccttttaa atcatcatca tctactttaa tttcaccatg agtatacgaa
    15121 ccttttttat tagttttaac cattacacat atcagcataa ttccacccct tattttcatt
    15181 tttgagagcc acaaaacact tcaacaatta atttatatta aaagacattt tgcaactctc
    15241 taaactgttt taattagata ttttccctat ataaaaatcc ttttaatctt tctatttctt
    15301 tgcttatttc atcattccaa atttttatag ctctacctcc atgtcaagcc actctttgca
    15361 tccatcaaca catttttcat ctacatcaca aatttcatat cgatattgac aactgcagta
    15421 aatacaagta caattatcac caatttcgtt tataaattca gccaactctt ttttactcat
    15481 attttttatc atttcaaaat tagtcattct taacctcctc tttttctctc aataatttac
    15541 gagctttttc ttcatcataa ctgcaaatat ttctaccatc acaataatga caaagtaatc
    15601 tagtattttt agtaacgtta ggaaacattt ttttgcaaat ctccaatcta tcatccattc
    15661 ttatacacct cttttgttat cacaattttc acactctttc agattcaatc tatactcata
    15721 aaccctacca gctataaaac taattcctat cagtagcgca ctagccaaga tattcattgt
    15781 atctcatcct ttctatcatc aatcaatata ctaaatccac aagaacattc tctataatat
    15841 gtgtgttctt caactattaa tttaccttca ttgtttccta tcttatcatt accacagttg
    15901 gggcaataac aatacttttc tccaagtttt ataatatctt ttaatttcat ttctcaatat
    15961 ctccttgaac tttcttattt tttcttttgc aagctaacat ttgtgataca aattcataaa
    16021 acattctctc catctcttct tcttctgaat ttacacaaac tttgaaaaac tccttgtctt
    16081 tagttaactg catttgtact ttcattctca agtttctcct tactctctaa aaattctttt
    16141 ctaacttctt ccaaattctc gtattcatta ccaactacta tatattcttt gctattcatt
    16201 ataattgctt tacttgtaag ttcctcccat ttgtcttcaa attgctttaa ataatgccat
    16261 tcaacatata atccaagagc ataattttct tgtcttacaa ttccatattc atatttattt
    16321 ttagtattat agggttcttt caatatatct ccctcataaa tttcttcatt atttccagac
    16381 caacatttcg agaatactcc aacattagat actttttgcc attcgcaatc ttcttttagc
    16441 ataaataaac agtctacagc attgctccac atttgctaat accatctgtc acctcttgaa
    16501 tatatctaac tttccaacca tttttagtag tctttcctgt tcttgctaga cgagtaatat
    16561 gaagttctgt aaaatataaa tatttacttg cttcgacagc actgacaaat attttactct
    16621 ctccagtaat gatgttagta cactctactt tcttactttt tcttccttgc cctttggaat
    16681 ttattctacc aattaaattt ttttgtttat ttttatttat catttctctt agattacatt
    16741 caatatctcc tatatcttta gttctttcta aaaatgttcc tgcatccatc caaatcttag
    16801 ccatatttag ttccccctcc ttattcaact ggcatttcaa acactttctc ttgattatgt
    16861 ctaactctgc aagaatctat ataatccgtt cttactccat tttctataaa ctcttgtatc
    16921 tcatttaaca cttgtatagc tctttcatta ctttcatatt cacccatttt tctaatacca
    16981 tcttcaaaca ttgcatatac tcgattatta cttatttcaa ctctattaac tctcattaaa
    17041 tctaatctat cttgacttct aattattatc atttctattc ctccgatata tttattttta
    17101 tttaacttct aggaagtaat attgtataac tactccctag attatttaac ttaaaaaggt
    17161 atatcatcat catctattgc ttgaaaccct tgagggttta atcctggtgg tatatattct
    17221 tgcttagggt tactatcact tttactagaa agcaattcta aagcatttac attaacttta
    17281 gtaatagatt tccagcaacc attttcatct ttgtaattat atatatttaa ctctccaaca
    17341 gcatatatag gcttaccttt aacaagatat tgcactaaat tctctacatg ttttcctaat
    17401 tgctcacatt gaataaaatc agttatttta tttccatttt tatctttaaa ccttctatct
    17461 actgccattg aaaaggttat ttttggagta cccgaatttg gaaggtactt caattc
//
LOCUS       DAEMHZ010000081        17310 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000081 DAEMHZ010000000
VERSION     DAEMHZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.081"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            203..1786
                     /gene="asnB"
                     /locus_tag="KPK72_002464"
     CDS             203..1786
                     /gene="asnB"
                     /locus_tag="KPK72_002464"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2489670.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(1851..2447)
                     /locus_tag="KPK72_002465"
     CDS             complement(1851..2447)
                     /locus_tag="KPK72_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG2489671.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDCMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2474..3001)
                     /locus_tag="KPK72_002466"
     CDS             complement(2474..3001)
                     /locus_tag="KPK72_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG2489672.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            complement(3363..4361)
                     /locus_tag="KPK72_002467"
     CDS             complement(3363..4361)
                     /locus_tag="KPK72_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG2489673.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            5038..6237
                     /gene="hadA"
                     /locus_tag="KPK72_002468"
     CDS             5038..6237
                     /gene="hadA"
                     /locus_tag="KPK72_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG2489674.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            6276..7076
                     /gene="hadI"
                     /locus_tag="KPK72_002469"
     CDS             6276..7076
                     /gene="hadI"
                     /locus_tag="KPK72_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG2489675.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            7091..8317
                     /gene="hadB"
                     /locus_tag="KPK72_002470"
     CDS             7091..8317
                     /gene="hadB"
                     /locus_tag="KPK72_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG2489676.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            8317..9444
                     /gene="hadC"
                     /locus_tag="KPK72_002471"
     CDS             8317..9444
                     /gene="hadC"
                     /locus_tag="KPK72_002471"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG2489677.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            9492..10625
                     /locus_tag="KPK72_002472"
     CDS             9492..10625
                     /locus_tag="KPK72_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2489678.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            10658..11443
                     /locus_tag="KPK72_002473"
     CDS             10658..11443
                     /locus_tag="KPK72_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2489679.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            11462..12499
                     /locus_tag="KPK72_002474"
     CDS             11462..12499
                     /locus_tag="KPK72_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2489680.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            12982..14613
                     /locus_tag="KPK72_002475"
     CDS             12982..14613
                     /locus_tag="KPK72_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2489681.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(14748..15674)
                     /gene="fba"
                     /locus_tag="KPK72_002476"
     CDS             complement(14748..15674)
                     /gene="fba"
                     /locus_tag="KPK72_002476"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG2489682.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            16004..16333
                     /locus_tag="KPK72_002477"
     CDS             16004..16333
                     /locus_tag="KPK72_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG2489683.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            16333..16536
                     /locus_tag="KPK72_002478"
                     /pseudo
     CDS             16333..16536
                     /locus_tag="KPK72_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            17167..17274
                     /locus_tag="KPK72_002479"
     CDS             17167..17274
                     /locus_tag="KPK72_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2489684.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
BASE COUNT         6685 a         2193 c         3109 g         5323 t
ORIGIN      
        1 atgaagaata aaaaattcaa tgttataaca ctgataatac ttgataatca tattgatgcc
       61 attaataaag agcagattag tactaattca aaaagaattt actttgaaaa aagtcgattt
      121 ttcagtgatt ataagtgaat aaatatatat aaacatttaa taaatttaga agttaaatgt
      181 tttaatacga aaggatacac atatgataaa tttagaagaa aagtatttaa cacgaaaaga
      241 cacatatatg ataaaaattc tagaatacaa tgggcatatt cgaaactgga aagttctttg
      301 tgaagaactt ggaatcgatt cttcattatg tagagatgag cgtgaacgag caattttagt
      361 ggaagcatat aagacatggg gatatgatat ggcaaatcac atgcatggaa tgtttgcttt
      421 tgcattatgg gatactgtgg aaaagaaact cttctgccta agagaccagt ttggggtaaa
      481 acctttctat tattatgaga ctgaaaatgg gaagcttctc tatggttcat ctattcgtaa
      541 gattatggag caaccaggat ttgtgaaaga gttaaatgaa gacatgttac agatttatat
      601 gtctttcaca tatgtagcag gtgaaaatac attcttccgt ggagtaaaga agcttatgcc
      661 aggacattac cttgtctatc agaataaaac agtatgtatt ggtcgttatt ggaaacctga
      721 atttcatcct gaccacagca agtctattga ggaatggacg gatgaaattc atacaacact
      781 tcagaaaatt atgccagagg taaaatcaga gaatgaaact gcagagttat tcctatcagg
      841 aggtgttgat tcatcttata tattggcaat gtcagatata gaaaaaacag gttcatgtgg
      901 ttatgaggaa gagcgttttg atgaatcaaa attagcacaa cagacagcta atattcttgg
      961 gtgtaaaaat tcgagatata taattacacc tgaagagtat tttgcgtcag taccttatgt
     1021 aatgtaccat atggagcaac caactggaga tgcatctgct atcgtttttg ctattggatg
     1081 caaggcagca gctgaaaaca caaatatctg ttactctgga gaaggagcag atgagttttt
     1141 tggtggatat aatatatacc ataaggcaga gtgttatgga gagaatttga agacatttta
     1201 tattggaaat acaaacatta tgaatgaggg tgagaaaaga aaaattctta agaaatacaa
     1261 tcctgatgtg cttcctattg aagtggtaaa aggaatttat gaagaaacag aagaactaga
     1321 tacacttact aacatgtcaa atgtagatat tcaaatatgg ttggaaggag atatttattt
     1381 taatatagat aaaatgagta ctgcagtagg attggaagtc agaatgcctc ttacagatac
     1441 aagaatattt gatattgcat ccagaatacc atcagaatat aaagtgaaag gaaaagagaa
     1501 taaaattgcc tttcgtacag ctgcatcaaa aacattacca gaagagattg cttttagaaa
     1561 taaattagga tttattgtgc caattcgtat atggatggct gatgaccaat ataataagga
     1621 tatccgagat aaattcaata gtgagatagc agataaattc tttaacatag atgagattaa
     1681 tgagattttt aatgactaca ttagtggaaa ctctgataat tggagaaaga tttggatgat
     1741 ttacacattc ctagtgtggt atgaaatcta ttttgtaaaa tgttaataca agaggaagaa
     1801 tcacctgaaa gaccattcag gtgattctta atgttttata attttacttg ctaattctta
     1861 tgtctaaccc ttaatggtat atcaagtttt tttgccagtt gtttagattc ttctatttct
     1921 tctggagata tttcatctac tactgaaaaa gctacttctt ttatatattt tttgcaatct
     1981 cttgcaaaat ctaacataca atcaaatgat ttttctccaa actttggttg agtaactcta
     2041 ttataagctt ctttatttgg ggcattaaga cttattgata ctgcatctat aacatttttt
     2101 aacagaatag ctgttttttt gttatgtatt aaatcactta gaccatttgt atttattctt
     2161 atctttatgc tacttttttc ttttataaac tttgctacct ctatcaattc atttattctc
     2221 attaatggtt cgccatatcc acaaaatacg atttcatcat agtcgtctaa attgtattta
     2281 ttgaaggctt cttttacttc atctacactt ggctcatgct ccaaccataa actaccacta
     2341 tttgcaactt catctttttc gtttcttata caaaaaacac aactacatgg acatgtatta
     2401 gttatattta catagatact attttcaatt gtatataaga tattcataat tttccctcca
     2461 taaatttaaa ttcttaatta aaataacgtt ttttaaaatc aagtttatca agtgccatac
     2521 ctaagattat aaaaacaata aaacttccta ttggctgtaa taagccaagt aagctcatta
     2581 caaatgcact tgtaaaactt ccaataatta taccttcagc aaacatgtat aaaactaatc
     2641 ctactatacc tgctacaacc gaagcaacta tatttctctt atttattatc ttgtcacttt
     2701 tagaagtgaa aaataataca agtattggtt ttatgattat agttggtatt acccagatag
     2761 cagcacctgt agataaatca gctagaccag caccaattgc agaagcagct atagcatagt
     2821 ttgttggcaa aatagttgca gctagataaa taaaagcatc tcctatatga acatatccat
     2881 tgtttccacc aactggaata tgtaatatat atgctattgt caggcatatt atggatgcaa
     2941 ataatgctga agtaacaata taggtagttt ttttagaagt aactttattc attttttcca
     3001 atatactccc tccctaaaaa tccagagcta acataattgt tatgttttct tataaatata
     3061 ttaatattaa tcatacaatt ttcatagttt gttaggtatt ttttttgtaa aatacagatt
     3121 gtacccattc aaatgtatat aattagaaaa gtaaaaatag gtaaaatgta tagagtagga
     3181 gtagttagaa taagttaata taaatttaat ttggtatgaa taagaggagt acaagtttaa
     3241 tataaaaaat aatataagag agtctactca atatatactg agctgactct cttatatttg
     3301 ggaaattaaa ggctattttg ttagataata gctaattaat gaaaaaatat atttataaaa
     3361 tactaattta ctctattagt agcagttcct gtttttataa cttctacagc actacttaaa
     3421 ctagaataaa ctatattttc aatcgctgta ctagtgaaga aaccaaggtg atgagtgaat
     3481 ataacttgtt ccatagaaag aagtttattt atttctgggt cagttaattc tccaggattc
     3541 atttttttgt ttaagaacaa tccttcagtt tcaaatgtat caagggcagc agctctaatt
     3601 tttcctgact ctagtgcttc tattaaatcc cctgtattaa ttaaaccacc acggccagta
     3661 tttactatgt aagctccatc cttcattata gctagagtat ctttatttat catatgtttt
     3721 gttccttcaa gtaaaggagt atgtaatgtt ataaggtcag cttcttttag aaggtcttct
     3781 aaagaatctt tgtaagttaa tatatcgttt aaatcactat ttggatattg gtcaaatgca
     3841 attacattag ctcctaggcc tttgaataat tttgcagaag tagcacctat ttttcctgta
     3901 cctataacac caactgtaat agatctaact tctgttccaa gaagttcagc tatccaacgg
     3961 taatcacctt cacagacttt tttctttact agaggagtct ttcttaaaag gttcatagct
     4021 tgagtaacag ctaactcagc tattgcatta ggtgaataag aaggaacgtt tgtaacgata
     4081 agtccatttt cattaactaa atcaaaatgt atcatgtcaa cccctgcagt tcttgaagct
     4141 ataactttaa ccccagcatc ttttaaagtt tcataaatat aaggattatc tatatagtta
     4201 gtttgttgta tagatatacc atcataccct ttagctttaa ctacattttc ttcagtcatg
     4261 ttttcaggat aaatatccac ttgaacatcc ttatgttctt cagaccattt ttttattact
     4321 ggttcttcat aatcgcgtgc tccaaatact agtattttca tatcaatacc ctcctgtaat
     4381 tattaaatta ttatggtaat ttttcattta gaaataatat gtagcatata aggttatatt
     4441 ttttatacaa aatttttaag gttaaaaatt tagttttgaa attgttttcc ctgtgttatt
     4501 ttgatattta gtaatttttt atttaatgta ataattactc taaaatttat caaagaaatt
     4561 gttatttttt agattaaata ttattaaaaa attatctaag tttgaaatta gttaactaac
     4621 ttgctgatta attacaatga taacatgtgt ttttgagttt agcaatgaaa actttttttg
     4681 aaatgtcaag ctttttcata aaaaaatata taaaaaaaac ttttataagc atagaaagaa
     4741 tagcaaaatg caatggatag tggcaagatg ccacgatatg aaaaagatta ttcaattgca
     4801 tattgttaaa aaacattcaa atcaagcgat attttgatta aatataaagt tggcacgata
     4861 aatgcttaac aatataggtg taagacaata aaacaataaa aaataatttt aataattttt
     4921 aagttatttt agataagatt aggaactttg acatcagaca gttaatatca gaaaaaagta
     4981 ttttatgagt gatttaatcg aaattttata attgatacta ttaaggagga aaacgttatg
     5041 cttttagaag gagttaaagt agtagaactt tcaagtttca tcgcagcacc atgttgtgca
     5101 aaaatgttag gtgactgggg tgcagaggtt attaagattg aacctataga aggtgatgga
     5161 ataagagtta tgggtggaac atttaaatct ccagcatcag atgatgaaaa ccctatgttt
     5221 gaattagaaa atggaaataa aaagggtgta agtattaatg taaaatcaaa agaaggagta
     5281 gaaatattac ataaattatt atcagaagca gacatatttg taactaatgt tagagttcaa
     5341 gcattagaaa aaatgggtat agcttatgac caaataaaag ataagtatcc aggattaata
     5401 ttctctcaaa tattaggata tggtgaaaaa ggacctttaa aagataaacc aggatttgac
     5461 tatactgcat acttcgcaag aggaggagtt agccaatctg ttatggaaaa aggaacatct
     5521 ccagcaaata cagcagcagg atttggtgac cactatgcag gtctagcact agcagcagga
     5581 agtttagcag cattacataa aaaagctcaa actggtaaag gtgagagagt aacagtaagt
     5641 cttttccata cagctatata tggaatggga acaatgataa caacagcaca atacggaaat
     5701 gaaatgcctt tatcaagaga aaatccaaac agcccattaa tgactacata taaatgtaaa
     5761 gatggaagat ggattcaatt agctttaata caatacaaca agtggttagg caaattctgt
     5821 aaggttataa atagagaata tatattagaa gatgatagat ataataacat agattcaatg
     5881 gttaatcatg ttgaagattt agttaagata gttggagaag ctatgttaga aaaaacatta
     5941 gacgagtggt cagctttatt agaagaagca gacttaccat ttgaaaaaat tcaaagctgt
     6001 gaagatttat tagatgacga acaagcttgg gcaaatgact tcttatttaa gaaaacatac
     6061 gatagcggaa atacaggtgt cttagttaat actccagtta tgtttagaaa tgaaggaatt
     6121 aaagaatata caccagcacc aaaagtaggt caacatactg tagaagtatt aaaatcttta
     6181 ggctacgatg aagagaaaat aaataacttt aaagatagta aagttgtaag atattaatta
     6241 gtaattctag attttttaaa aggagaatgc taaagatgta cacaatggga ttagatatag
     6301 gttcaactgc atcaaaggga gtaatcttaa agaatgggga agatattgta gcttctgaaa
     6361 caatatcctc tggtactggg actactggac catcaagagt tttagaaaaa ttatatggca
     6421 agacaggtct tgcaagagaa gatattaaaa aagttgtagt tacaggatat ggaagaatga
     6481 actattcaga tgctgataag caaataagtg aattaagctg tcatgctaga ggggtaaatt
     6541 tcataattcc agagacaaga accattattg acataggtgg tcaagatgca aaggtattaa
     6601 aattagataa taatggaaga ctattaaact ttcttatgaa tgacaaatgt gctgcaggta
     6661 caggaagatt tttagatgta atggcaaaaa taatagaggt tgatgtatct gaactcggaa
     6721 gtatatctat gaattctcaa aatgaagtat caataagcag tacatgtaca gtatttgcag
     6781 agtctgaggt tatatcacat ttatctgaaa atgcaaaaat tgaagatata gtggcaggta
     6841 ttcatacttc agtagcaaag agagtttcta gcctagtaaa aagaatagga gtacaaagaa
     6901 atgtagttat ggttggtgga gttgctagaa atagtggtat tgtaagagct atggcaagag
     6961 aaatcaacac agaaattatt gtacctgata tacctcaatt aactggtgct ttaggagcag
     7021 cgttatatgc ttttgatgaa gcaaaagaat cacaaaaaga agtgaaaaat atataaaaag
     7081 agggatgaaa atgtctgaaa aaaaagaagc tagagtagta attaatgatt tattagctga
     7141 acaatatgca aatgcattta aagctaaaga agaaggaaga cctgtaggtt ggtcaacatc
     7201 agtatttcct caagagttag cagaagtatt tgacttaaac gtattatatc cagaaaacca
     7261 agcagctgga gtagcagcta aaaaaggttc tttagaatta tgtgaaatag ctgaatctaa
     7321 aggatattct attgacctat gtgcatatgc aagaacaaat tttggtcttt tagaaaatgg
     7381 tggatgtgaa gctttggata tgccagctcc agatttccta ctttgctgta acaatatatg
     7441 taaccaagtt ataaaatggt atgaaaatat ttcaagagaa ttagatatac ctttaataat
     7501 gattgataca actttcaata atgaagacga agttactcaa tcaagaatag attatattaa
     7561 agctcaattt gaagaagcta taaaacaact agaaattata tcaggaaaga aatttgaccc
     7621 taagaagttt gaagaagtaa tgaaaatatc agctgaaaac ggaagactat ggaagtattc
     7681 tatgagttta ccagcagatt cttctccttc tccaatgaat ggatttgact tatttactta
     7741 catggctgta atagtttgtg ctagaggtaa aaaagaaact acagaagcat ttaagttact
     7801 tatagaagaa ttagaggaca acatgaaaac tggtaaatct tctttcagag gggaagaaaa
     7861 atacagaata atgatggaag gtataccttg ttggccatat ataggataca agatgaaaac
     7921 attagctaaa tttggagtta acatgacagg tagtgtttac ccacatgctt gggcattaca
     7981 atatgaagtt aatgatttag atggaatggc agtagcatat agtactatgt ttaacaatgt
     8041 aaacttagac cgtatgacaa aatatagagt tgattcttta gtagagggta aatgtgatgg
     8101 agcattctat catatgaaca gaagctgtaa acttatgagt ttaatacaat atgaaatgca
     8161 aagaagagca gctgaagaaa ctggattacc atatgctgga tttgatggtg accaagcaga
     8221 ccctagagct ttcactaatg ctcaatttga aacaagaatt caaggtttag ttgaagtaat
     8281 ggaagaaaga aaaaaactta atagaggtga gatataatgg aagctatttt atctaaaatg
     8341 aaagaagtag ttgaaaatcc aaatgcggct gtaaaaaaat ataaaagtga aactggtaaa
     8401 aaagctatag gttgtttccc agtttattgc ccagaagaaa ttatacatgc agctggaatg
     8461 cttccagttg gtatatgggg aggacaaaca gaattagatt tagctaaaca atatttccct
     8521 gcatttgcat gttcaataat gcaatcatgt ttagaatatg gattaaaagg tgcttatgat
     8581 gaattatctg gagttattat accaggtatg tgtgatacac taatttgttt aggacaaaac
     8641 tggaaatcag cagtacctca tataaaatat atatcattag tacacccaca aaatagaaaa
     8701 cttgaagctg gtgtaaaata cttaatcagt gagtacaaag gcgtaaaaag agaacttgaa
     8761 gaaatttgtg gatatgaaat agaagaagca aaaattcatg aaagtataga agtttacaat
     8821 gaacatagaa aaactatgag agactttgtt gaagtagctt ataaacattc taatactata
     8881 aaaccatcaa taagaagctt agtaattaag agtgggttct ttatgagaaa agaagaacat
     8941 actgagctag tgaaagattt aatagcaaaa ttaaatgcta tgccagaaga agtctgttct
     9001 ggaaagaaag ttttattaac aggtatatta gctgattcta aagatatatt agacatttta
     9061 gaagacaaca atatatcagt tgtagctgac gatttagcac aagaaacaag acaattcaga
     9121 acagatgtac cagcaggtga tgatgcgtta gaaagattag caagacaatg gtcaaacata
     9181 gaaggatgtt cattagctta tgaccctaag aaaaaacgtg ggtcacttat agtagatgaa
     9241 gttaaaaaga aagatataga tggtgttatc ttctgtatga tgaaattctg tgacccagaa
     9301 gaatacgatt atcctttagt tagaaaagat atagaagata gtggaatacc tactttatat
     9361 gttgaaatcg accaacaaac tcagaataat gaacaagcca gaactcgtat tcaaactttt
     9421 gctgagatga tgagtttagc gtaagttcat taaaataaat ttactataaa atatgaaaat
     9481 tggaggaagc tatgttatat aacaaagaac aagaactttt aagaaaagca gtaagagatt
     9541 ttgttagtaa agaattagat actttaccag cagaaatgga taaaacaggt gtaatgccta
     9601 aggaattaat caaaaaatta gctgatgcaa aatttataag cagtaatatc ccagaagaat
     9661 atggtggtgg gggagcagga tatgtttctt acgctatagt aatggaagaa atagctagaa
     9721 gatgtgcttc aacagctact tttgttacag ctggttcttc tcttgcttca ttaccaatac
     9781 tttacaatgg tactgaagag caaaaacaaa aatacttaaa aggtatagca acaggtgaat
     9841 taataggagc atttggttta actgagccag gagctggttc tgatgcaggt ggacaacaaa
     9901 caacagcaga attagttgga gaccactata tattaaatgg tagaaaaact ttcataacta
     9961 atggaccatt ctgtgatgta gctatagtaa tagcagttac agatagaagt aaaggactta
    10021 gaggaacatc agcgtttata gtagaaagta aatgggatgg tttctcaaca ggagctcatg
    10081 aagataagat gggtataaga ggaactgaaa cttctgactt aatatttgaa aacgttaaag
    10141 ttccaaaaga aaacttactt ggaaaagaag gtcaaggatt taagatagca atgggtactc
    10201 tagaagttgg tagaataggt gttgctgcct tagctctagg aatagctcaa ggtgctttag
    10261 atgaagctgt taaatataca aaacaaagag ttcaatttgg taagcctata gctaaattcc
    10321 aaaatactca atttactata gctgatatgg aaacaaaagt ttgtgcagct agaggattag
    10381 tttatgatgc agcacaaaag agagatgcag gaatgagagt tgctcaagaa tctgctatgg
    10441 ctaaatacta tgcatcagaa attgcaaatg aagttgctta taaagcatta caacttcacg
    10501 gtggatatgg atttataaaa gattatgaaa tcgaaagaat gtacagagat gctagaatcg
    10561 tatcaatata cgaaggaaca tcagaagttc aaaaaatggt aatttcatca aacgtattaa
    10621 aataataaat aatctaatga aattggaggt atagactttg aaaatattag tttgcgtaaa
    10681 acaagttcca gatactaacg aagtaagaat aaataaagaa acaggtactc ttataagaga
    10741 tggagttcca agtatattaa acccagatga tgcaaatgca ttagaggaag ctttaaaaat
    10801 taaagatgag catgatgatg taactataac agttataaca atgggacctc ctcaagcaga
    10861 ctttatgcta agagagtgtt tagctatggg tgctgatgat gcaatattat taagtgatag
    10921 agcatttgga ggagctgata cttgggctac atcaaatact attgcagcgg gaatatctaa
    10981 agtaggtgac tatgacataa tatttgcagg aagacaagca atagatggag atactgcaca
    11041 agttggacca caaattgcag agaaattaga cattcctcaa gttacatatg tacaagattt
    11101 caaaatagaa ggaaaagata taatagttca aagacaatta gaagatggat acgaattaat
    11161 aaaagtaagt acacctgtac ttttaacagc agtaaaagag ttaaatacac ctagatacat
    11221 gtctgttgat aaaatagtaa aagcatataa acatgatgta aaagtttgga caattgatga
    11281 tttagatgta aataaagaag aagttggttt aaaagcatca ccaactaaag tatttagatc
    11341 atttactcct gaaccaagag gaaaaggaga aatcttagaa ggtaaagctg atgaaatagc
    11401 tagtaaaata ataatcggtc taaaacaaaa gcacattata taagttggag gagtacaaac
    11461 tatgaatgat ataaaagatt taagttctta taaaaacgta tggatatttg cagaacaaag
    11521 agaaggaaaa atagctccag tagttataga attattagga gaaggaagaa aattagctaa
    11581 agaagtagat gcagaacttt gtgcaatatt attaggaaaa gatgttgatg gattagctaa
    11641 agaattaatc acttttggag ctgacaaagt ttatgttgca gatgatgctc ttttagaaaa
    11701 atatacaact gatgcatata caaaagtaat aaaagatgca atagatgaaa taaaaccaga
    11761 aataatgctt ttcggagcaa ctcatatagg tagagactta gcacctagaa tagcttcaag
    11821 agttggaact ggattaacag ctgactgtac taagttagaa atagacccag aagataagaa
    11881 aataaaacaa actcgtccag catttggtgg aaacataatg gctacaatca tttgtccaaa
    11941 tcatagacct caaatgtcta cagttagacc tggtgttatg gataaagctg aaaaagacga
    12001 aacaagaact ggtgaagtta tagcattaga ctacaaaata actcaagatg atataagaac
    12061 tactgtttta gaaacagtta aaactaagaa agatttagta tctcttacag atgcaaatgt
    12121 tatagtatca ggtggtctag gattaggtgg accagaagga tttgaaatgc ttaagaaatt
    12181 agctgacaaa ttaggtggag tagttggttc ttctcgtgcg gctgttgatg ctggatggat
    12241 agaccattct caccaagtag gtcaaacagg aactactgtt aaaccaaacc tatatatagc
    12301 ttgtggtata tcaggagcaa tacaacattt agcaggtatg caatcatcag atttcataat
    12361 tgctataaac aaaaacccag cagctccaat tttagaaatc gctgactatg gagtagttgg
    12421 agacttacat gaaatagttc caatgcttat agaaaaatta gatagtgttg atgatttatt
    12481 agaagctata aaagcttaat agatacaaac tatataaata ttactgattg aataagtctt
    12541 attagttagt aacataatta ttaacccttt gataatttat tgctagtgtg tatttgtcag
    12601 ttattaaaaa gtctgtttga ataaaatttt ataatacaag ataatctgga taatccttat
    12661 atgtatattg cttacaaatt gtgagttata ttctgtaagg attatctttg tttttttaaa
    12721 taaatcttaa atttaattaa attgtattaa atagacaaat aattactaaa tatgtgataa
    12781 aatacaatta aaagcaaaat gaaattttta acaaactata atcattaaaa ttatatctta
    12841 gttacgtttt aatgaaataa actacttgaa aagacgaata ttataaggag ttgattatat
    12901 gtgctgatgg tatttattgt aagcaaacaa taaattttgt atgtagttca ataaattact
    12961 taatgaaagg tgcgaatatt aatggataat aatttaatag ggcttataga acatgtagaa
    13021 aatccagcca ttttatgtaa agaatcaggc gaaataatat actgtaatca tctaatagat
    13081 agtattttta gttttctaga tataaaaaag cctagaaata taaatgaatt agattcaaat
    13141 tttgacaaaa cagaaatact gacagatagt aaaaagaaaa tagctttcag agagttaagg
    13201 atgactgcac atatatacaa tatgaaagat aataataatg aaaataatat agtttattta
    13261 tttgagaagt ctctaatatc tgataaagta attgaagata taatagagca tatagatgaa
    13321 gttgttgttg tgtttaacaa agatggggta attgaaaaga tgaacactgt cagtgatgag
    13381 atattacctt ttaaaagaac tgaggtactt ggtagaaata taacagacct tgtgagacaa
    13441 ggattagttg aagaaccaat aatattaaac atgcttaaag tgaagaaaaa gatttataga
    13501 aatatcgttt acccagatgg gaagcttatt gcatatacgg cagttccaag atgggattct
    13561 aagggaaagc ttacaggagg agtactaaca gggagagata tttcaagggt tattaagctt
    13621 gaatctcaga taaaatatag tgatatatca gaagacacag agtatataag tcaaagtaag
    13681 attatggaca atataaaaaa agttgttaag agagctgcag cctcagattc ttctatattt
    13741 ataaatggag aatcaggtgt aggaaaagaa ataatagcaa gaacaatata taagtatagt
    13801 tcaaggagag acaaaccttt tatagctata aactgtggag ctataccaaa tgaattatta
    13861 gagtcagagt tttttggata tgaagaaggt tcttttactg gagctaagaa aaaaggtaaa
    13921 aagggacttt ttgaagaggc gaatggtgga actattttct tggatgagat aggtgagtta
    13981 ccaatgcaaa tgcaaaaaaa attacttagg gttatacaag aaaatactat tactagaata
    14041 ggtggtagta agcctataaa aatagatgtt agatatataa gtgctactaa tatttctcat
    14101 gaagacctta gaaataacct taaatttaga caagatttgt actatagatt aagtgttatt
    14161 ccagtaaaaa tacctcctct tagagaaaga aaagaggata tagtaccatt agtaaattat
    14221 ttcttaaaac tttacaatga aaagtacaat agagaagtag aagtttcgcc taaagttatt
    14281 gagttattag aagaatattc ttggccagga aacataagag aattaaaaaa catcattgaa
    14341 agatttgtag tattatcagc taaaaatgta attggagaag atgaatttaa catgctgata
    14401 aatttagata tgattgataa tgaaacagat gatttatcac caatagtggt aaatggaatt
    14461 atgaatctta atgatgcata taaaatagtg gaccaaataa tgataagtaa ggctataaat
    14521 aagtatggtt caataacaaa ggctgctgaa gttataggta tatatccttc taccatccat
    14581 agaaagataa agagtggaca tattcacgtt tagctgtcag tttaaaaaga caatataatt
    14641 aaaatattat aagatacata attgatacaa cttaaaagag atggcttaaa aataaattta
    14701 attactttaa gtcatctctt ttcttttata aatatttaaa agttatatta gtttatagag
    14761 ttagcagaac ctaaaacatc atctatcttg ctttcaacta cagcttgtat agcatctcta
    14821 ccagcaccaa tgtattttct tgggtcaaat tcttttggat tttcagctaa gaattttctg
    14881 atagcagcag tcatagctag tcttaagtca gtatccatgt ttattttaca aacagccata
    14941 gaagatgctt ttcttaacat atctacaggt acacctttag caccagctat atttccacca
    15001 aattcattac atgtagcaac agcgttttgg tcaacagcag atgcaccatg aagaactatt
    15061 gggaaacctg gtaatttgct ttggatttct tctagtatat cgaatcttaa tttagcttct
    15121 cctttgaatt tgaaagcacc atgagaagtt ccaatagcta ttgctaatga atcaacacct
    15181 gttctttcaa cgaactcaac tgcctcagct ggttgagtat acttgtgaac atcagaagta
    15241 acatcatctt ctgttccagc tagaacacca agttctgctt caacaacaac acctttagag
    15301 tgtgcatatt caacagcttc tttagttatt ctaacatttt cttcaaagtc aaaatgagaa
    15361 ccatctatca taactgaaga gaaaccagca tcaatacaag ttttaatagc atccatattt
    15421 ggaccatggt ctaagtgaag agctacatct acacctattt catcagaagc agccttaacc
    15481 atttcaacta atgtatgagg tccagcatat ttaacagctg acatagaagc ttgtatcata
    15541 acataagaat ttttagcttt agcagctttt aaaacacctt gtaattgttc taagtcactt
    15601 atgttaaatg cacctatagc aaatccacct tcgtaagctt ttttaaacat ttcttttgta
    15661 gtaattaatg ccattttaaa ctcctcctat taacttttaa aatttaccca tcacttatat
    15721 tataacgtta aatcaaaaaa agtatatagt aaattgtaat aactataaat tacaagtata
    15781 atgttaaata gaagacaata ggattaaata aaataataaa gaatataaac aatttaggaa
    15841 aactagctat ataatgctaa ctataagggt aggaactacc ttattagttt ggagatactg
    15901 ataacaatag ttgtcttgac caagaagttg ccacttcaaa cgttatcgaa gggaattaag
    15961 tggatgataa ttcactaaaa atttaaaata aaggagataa actatgccac agataaaaat
    16021 aagaggaatt aatgaaaatg atatatgtaa aataagtgaa aaaatgataa atgatttagt
    16081 tgaagctgta aaatgtccaa gagactactt tgaaatagaa tgtataaagt cagttgcaat
    16141 aagagatggt aaaatagcag atgtatatcc ttttgttgaa gtagcttggt ttgatagagg
    16201 acaagaagta caagatatag tagcaagaat aataactgat agtataagaa ataatttaga
    16261 tgtagaaagt atggatttag catttacagt atttgaaaaa gaaaaatatt atgagaatgg
    16321 agagcatttt taatggctaa aaagcaacgt atagataaaa ttctttcaaa tttaggttat
    16381 ggaagtagaa gtgaaataaa gaagtattgt aaacaagggt ctgtagtagt aaatggaagt
    16441 gaagtttcaa atccaggaac tcaagtagac actgaaaatg atgagatatt atttaatgga
    16501 gaagaagtta tatatagaga atatatatat ttgattaaaa aatagctacg tacactcgtg
    16561 actttagtca taagttagta gctaaaatag tcagcatata gggaaaccta tatgtagaga
    16621 taggatagaa cctatccaat actacttgaa ttgctggaaa cccctaaagc taaccaaact
    16681 aaaacataag gatgaaataa acctaagtgt gaaagttacg aaagtagaaa aaattggtta
    16741 gatggtataa ggttaaaccc taagtacttt aaaatgggca atcagcaggt aaactccgaa
    16801 taggagaaac ttcaacgact attcctcttg agggaagtac actacaagtt attggtagtg
    16861 gaagtgggta gaccttaacg gataatgccg aaggataaga tatagtctgt gcttgcatga
    16921 aagtgtaaga agttcataag agaactgcat aggtagtagc gaacttatgt gaacgacaac
    16981 ctcaaaaatg atagaagtta agacttatgg ttcttatata tgttatttaa aattaaatat
    17041 aaaaaacctt caaaatactt gttttaagta attgcatatt ttaatattat gataggtgat
    17101 gaaaaatgga agttactcat ggtagaggat atgcatatag tatgtcaaat atagacagcc
    17161 agcattatgg ggagacatct ttggaatcct agttatttcg ttgctactgt atcagaaaat
    17221 acagaggagc agataagaaa atatattaat aatcagaaaa aacaagtaaa gtgaggtggt
    17281 agtagtggaa aaagcttata agtttagaat
//
LOCUS       DAEMHZ010000082        17309 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000082 DAEMHZ010000000
VERSION     DAEMHZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.082"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(218..742)
                     /locus_tag="KPK72_002480"
     CDS             complement(218..742)
                     /locus_tag="KPK72_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2489685.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(807..1559)
                     /locus_tag="KPK72_002481"
     CDS             complement(807..1559)
                     /locus_tag="KPK72_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2489686.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEVGKNILDRL"
     gene            complement(1590..2888)
                     /locus_tag="KPK72_002482"
     CDS             complement(1590..2888)
                     /locus_tag="KPK72_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG2489687.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2905..5169)
                     /locus_tag="KPK72_002483"
     CDS             complement(2905..5169)
                     /locus_tag="KPK72_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG2489688.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5424..6242)
                     /locus_tag="KPK72_002484"
     CDS             complement(5424..6242)
                     /locus_tag="KPK72_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2489689.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKRLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6235..6933)
                     /gene="cbiQ"
                     /locus_tag="KPK72_002485"
     CDS             complement(6235..6933)
                     /gene="cbiQ"
                     /locus_tag="KPK72_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG2489690.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6936..7220)
                     /locus_tag="KPK72_002486"
     CDS             complement(6936..7220)
                     /locus_tag="KPK72_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2489691.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7210..7962)
                     /locus_tag="KPK72_002487"
     CDS             complement(7210..7962)
                     /locus_tag="KPK72_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG2489692.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8144..8336)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8468..10240)
                     /locus_tag="KPK72_002488"
     CDS             complement(8468..10240)
                     /locus_tag="KPK72_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG2489693.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10254..11183)
                     /locus_tag="KPK72_002489"
     CDS             complement(10254..11183)
                     /locus_tag="KPK72_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2489694.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11411..11599
                     /locus_tag="KPK72_002490"
     CDS             11411..11599
                     /locus_tag="KPK72_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489695.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11592..12293
                     /locus_tag="KPK72_002491"
     CDS             11592..12293
                     /locus_tag="KPK72_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489696.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12295..13218
                     /locus_tag="KPK72_002492"
     CDS             12295..13218
                     /locus_tag="KPK72_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2489697.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13375..14298
                     /locus_tag="KPK72_002493"
     CDS             13375..14298
                     /locus_tag="KPK72_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489698.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKKALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14291..15028
                     /locus_tag="KPK72_002494"
     CDS             14291..15028
                     /locus_tag="KPK72_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489699.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15039..15812
                     /locus_tag="KPK72_002495"
     CDS             15039..15812
                     /locus_tag="KPK72_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489700.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15828..15980
                     /locus_tag="KPK72_002496"
     CDS             15828..15980
                     /locus_tag="KPK72_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489701.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16353..16961)
                     /locus_tag="KPK72_002497"
     CDS             complement(16353..16961)
                     /locus_tag="KPK72_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG2489702.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17139..17255)
                     /gene="rrf"
                     /locus_tag="KPK72_002498"
     rRNA            complement(17139..17255)
                     /gene="rrf"
                     /locus_tag="KPK72_002498"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5577 a         2991 c         2191 g         6550 t
ORIGIN      
        1 ttaaaaataa atatataaac aatcataata aaattttttt aataaaactt tattttttag
       61 ttaaaatcat acaatatata gatttccaca atacaaataa attaaaaagt gaaatgtatt
      121 tgtctaaaaa cataataaca tttcacttta tcctcatttt taagtaatat atatttttaa
      181 tttttattta taactaaatc tagtttttat ataaatttta gtttttttct acatctgagt
      241 aatctattcc aaaagtttct acagtaacag atttcataac ttgtggagtt tgtggcttat
      301 cagctgaatc tgttctaact gatgctattt catcaactgt atccatacct tctatcactc
      361 taccaaaacc tgcatactga ccatctaagt gtggtgagtt tttatgcatt atgaaaaatt
      421 gagaaccagc tgaattaggt gccatagttc tagccattga tatgactcct ctttcatgtt
      481 ttaaattgtt agtaaatcca ttaccactaa attcaccttt tattgagtga cctggaccac
      541 ccattcctgt accatttggg catcctcctt gtatcataaa tcctggtatt actctatgaa
      601 atattattcc attgtaatat ccttcattta ctaatgatac aaagttttta actgtatttg
      661 gagctatatt tggatataac tcaattttga tttcttttcc attttccatt tctatagtta
      721 ctataggatt tttattttcc ataatttatt accttccttt tcattagtta aattacataa
      781 ttacatttat tatattatac tctaaattaa agtctatcaa gtatattttt tcctacttca
      841 tatccttctt gataaatagt tttacaaaac tcatcatcaa agttaagcgt gtctttagga
      901 gcaaattgag tattaggtct aactactatt attttatctt catcatatac ttttttaata
      961 tcttctggca actcataatt atattttgtg gatattaaaa taattttatc cacattatcc
     1021 ccaattaatg gttcaattaa tgcactccta attaaacctc catctaattt gaaaccatca
     1081 taaattcctt cttgcacatc tttaacaaat tgttctttaa atccaagagt tgcgtttgga
     1141 tttgatggta acaacccact gtatgaaatg ctgataatgg cttcattacg aggcattttt
     1201 gatacgtcaa caatcttttc tgatactact ttattatcaa cttcaacata attaacataa
     1261 aagttcattt cttctatatt actatcatct agttccctta ggttgtctat agcaggagaa
     1321 ttatccacag tattatttac tatttttaca tttccattct ggatatcttt tatatttgtc
     1381 cacattttct ccaagttatc aacattccct gtgtacaaat aatatccatt tattgctcct
     1441 attgatgtcc ctgaaatcga ataaaattta tttattcctc catcatacaa ggctttaact
     1501 actcctgctt gataagcccc ttttgctcct ccaccttcta aacataatcc taatttcata
     1561 gtctcatctc caaattgttt atcttatatt tatatttcaa aaaacttact aaatgtttta
     1621 tatatactat agtggtcctc aacatttcct aattctctta tagaatgcat tgctaaaatt
     1681 ggacttccca catccacaga tggtatatct atatgagtag aagatattgg acctatagta
     1741 cttcctcctc tttcatctga cctattaaca aattcttgat actcaacatt agcttctcta
     1801 catatatttt tataaatagc tatagaatat ccatcactag tatatgcctg actagcattt
     1861 atctttataa ccggaccttt tcccattatt ggtttattag ttgggtcatg cttttctgga
     1921 atgtttggat gaatagcatg agctaaatct gatgatatca taaaagatga ataaatagac
     1981 tcaaagaatc cctctctatc cttacccatt gatatgcaaa ttctctccaa tatatttaac
     2041 aacatgtttg aatctgcacc ttgtttagta gagctaccaa cttcttcatt gtcaaataca
     2101 gccattacat ttacaccatg ttgaccttta gtattaacaa gagcattcaa actagcatgt
     2161 accattgcaa gattatcttg tctcccaata gatataaatt catcattagc ccctattaaa
     2221 cttccttttt caaattcata taaaaataaa tcaaaatcta taatatcgtc catatttaca
     2281 tctaagttag atgctatttg ctttataaga aaattatctt tttctaatgt atcatttaac
     2341 atcccaacta aaggtaacat atctttttgc ttattatatg agtgaccatc atttatactt
     2401 ctatttaaat gtattgctag atttggtatt atacaaattg gctttttaaa gtctataagc
     2461 ctttcatttg gttttaatat actatcacct tttaaaacta ctctaccagc tatcccaaga
     2521 ggtctatcca accatgtact taatattgct cctccatagc attctgtatt taacttcaaa
     2581 taagattttt cagcttctat ttctgcattt ggtttaatac taaatgtagg agaatctgaa
     2641 tgagagccta ttattctaaa tccatctctt tctatatctt ctgaatttac tacaaaagct
     2701 actatagctg ataaattctt agtaacataa tactttccac caactcttag attccatctt
     2761 tgattcaaaa ctaattcttc aaatccattt ttatctaaga tttccttagt actctcgact
     2821 gcatgaaaag acgacggact atcatatatg aagtctatta agtttttagc aaagtttata
     2881 ctatccattc ctcatttacc tccattataa aatatttagt gttgtatatt ctttatttat
     2941 ttttttaatt ccctgttggt caaaagctat agtaacatct gtaccaatta tagaaaccac
     3001 tgtcccaacc ccaaatttag gatgatgtac cttagaacca agttttatat catctggatt
     3061 agtttcttta ttagaatctt ttattgtagc attaacttta tctgcaactt tagatttgct
     3121 tatagtactc atatactttt tagtatattt atctaacata ttataacttg ctttagaata
     3181 gcttagttct ttttttactt tgtacaatct ttctatacat tcttctggca gttcttccat
     3241 aaatcttgaa gctattgcaa catttgtttt accatataat gttctcttct ctgtcaatga
     3301 catatatagc tcttctttag ctctagttat accaacataa caaagtcttc tctcttcttc
     3361 tatttgtgaa tcactcatag attttatagc tcttgatata ggaaataacc cttcttccat
     3421 acctgttaaa aacacaacag ggaactctaa acctttggat gtatgaattg tcataagcga
     3481 aaccctatca ttgtcttctt catcatttga ttcagatgta agtgctatac ttgttaggaa
     3541 tgtctctaag tctttatttt cataagtatc atcattacta ctttcaaact ccaaagctat
     3601 agaaataaat tctctcaaat tatcaattct gtcttgagct tcttcacctt tgcctgttaa
     3661 atctttttca tttttatttc ttatctctac aagctcgtcc atatatcctg tagtatccaa
     3721 gactttttct ataagtttgc taacaggata aacttctttt atagttctaa gagtccctat
     3781 aatatcaaca aatccactta tacttgccct tgccttagta gatatatctg aatttgtttc
     3841 tatatcaatt aatactgagt atatactttc ctgttttaag ttagctctat cttcaatttt
     3901 ttctatagtt ctaagcccta tacctcttct aggaacattt ataatccttt ttatagatat
     3961 atcatcttgt ggattttgaa taactcttaa atacgccaac aaatccttta tttcttttct
     4021 ctcatagaat tttgtaccac catatatatt gtatggaatt tgacttctat ttaaagcgtc
     4081 ctctacaggt cttgcctgtg catttgctct ataaagtact gcaaaatctt tataaggtct
     4141 attttctttt ctagctattt gtgcaattga atcagcaatg aaatcagcct cttctatttc
     4201 gctaccagtt aattgtatct ttataagttc tccttctttt ttttcactcc aaagcttttt
     4261 tctttttctt tctatattat tagatatgac tttattagct gcatctaata tcacttgtgt
     4321 agacctataa ttttgttcta actttacaac atgtacattg tcataatctt tctcaaattc
     4381 aagtatgttt cttatatctg cacctctcca tccataaata ctttggtcat catctccaac
     4441 aacacaaata ttttgatgtt ctcttgctaa taattttatc aattcatatt gagctttact
     4501 tgtatcttgg tactcatcta ccattatgta tctaaatcta cttctgtaat atgctaacac
     4561 ttcatcatta gccttgaaaa gttctacagt tttaagtatt aaatcatcaa agtcaagtgc
     4621 actatttctt tttaaacggt cttgatatag cgcataaata tctgcaatct taaccattct
     4681 attatctgcc atattgatat ctttaaactg ctttggagta taaagtttat cttttgctcc
     4741 tgaaattgca cttataactg cttttggttc aaacacttta tcacttaaat ttaattcttt
     4801 tagacagtct ttaaccaatg taacttggtc tgctgaatca taaattacaa aacttctatt
     4861 gtaaccaatc ttatttatat cttttcttag tatacgaaca cagcaagaat ggaatgtact
     4921 tatccacata tccttagttt caggacctat attttgttca actctctctc tcatttcatt
     4981 tgcagctttg tttgtaaatg ttatagctaa tatatttggt gcttgtacac ctttatcttc
     5041 tataagatat gctattcttg ttgttaatac cttagtttta cctgaaccag caccagccag
     5101 tataagtact ggaccttcag tcttttctac tgcttctctt tgagcaggat ttaatgtatc
     5161 taaattcata tttctcctcc tgttaacagc cataaatata ttttaatcta aaatatgaat
     5221 tacttcaaat tataattatg tattatatta atagattatt tccaatatat tacaattaaa
     5281 taacattttt ccatataatt tatttcattg tttaataaaa aaaataacct tatgatttgg
     5341 ggaatcataa ggcaccttta aggggtctag gttaagggtt attatatata aatctatttt
     5401 tttaaaatag gtttagggct taattattta tttgtattaa gttctctttc cttccaatac
     5461 ttatacaaat cttcttcttt cttaaataag ggtaaattca tatttctatg tacttttact
     5521 aaccatgggg attctaggcc agcttgtata atattatttt catttataaa cacattttct
     5581 gcttttcctt catcaatcaa aatcccctta tccaatacat atatgtaatc acatatttca
     5641 tacattaaat tcatatcatg actggatatt actattttta tattattttt atttaatctc
     5701 tttattatat ctacaataga cctagtactt actgggtcta aaccagctgt aggttcatca
     5761 agtaatacaa tttcattttc cattgctata actgatgcta ttgccactct ttttttctgt
     5821 ccatagctaa gaaaatgtac tggcctgtct ataaaatcta ttccattaac agctactaga
     5881 gctttgttta ttctctcttt aattgttttt tcatccatac caatgtttct cattgcaaag
     5941 gctatatcat catatacttt tgaataaaaa atttgttttt ctgggtcttg gaagacaata
     6001 cctacatttt ttctaaggtt gtataaactt ctcttatcat attttaactt ttcttcttta
     6061 aaaagaattt ctccagatgt aggctttaat atacccatta aattcataaa taatgttgat
     6121 tttcctgaac catttgaacc tattatacct ataacatttc cctttgaaaa atccatatta
     6181 atgtttaata gtgcattggt atttttttca tattgatacg ttaaattatt tattttaaac
     6241 atcattatcc cctactatgt gaaatttacc atcatacaat tttatgtcta atgatataca
     6301 catatcatca taccttgtca taagtctttt gtacagcata cttccaagaa tacctaagga
     6361 tttatatgaa gtttttaaat ttatatatcc aaatcttagc tcttgtgact tgtatatttc
     6421 tgaaaattct tctaaaaata tgaatataaa cctatatatt aacattgata actcgacaaa
     6481 agtgtcaggt aaatgaagtt ttttaaaaaa gaatattaat tgattaaaag gtgttgtaag
     6541 tatacaaaaa tacacacaag tcaaacaaga catcgctcta aataacacat gaatagacat
     6601 gtcaatagat gcctttgata ttcctacatt aaaactaaaa aattcaaaac tatataataa
     6661 atctgcttta ttaaaagata tatttacaag agttattcct atactaagaa ataaaaagta
     6721 cattggtatt ctaagtaatt ttaaataatt ttttaaatct attccagcca cacaaacaac
     6781 caaaatactc attaaaatca taattccact taaaataaat atattttgta ttaccataga
     6841 agcaatcaga aaaattactc ctatggctac ttttttgttt ggatttactt tactcaatct
     6901 atttgtatat gcatatttat caattataag catgattaac ttctctttcc tttttgaaga
     6961 cctaatatgt atcctattac accagcccct aaagctgctt gtgcagagaa taataaactt
     7021 tctatttctc cacttggtgg ctcatatgga ctgctaaacc atggtttgta atttggattt
     7081 atcttagtaa tttcactttc agcttgacca tctgctcctc catattcagc acctgagttg
     7141 acaagtaatg ggaatataat aagagctact actaataaaa gtaataatat gttagttttt
     7201 gtttttgttt tagcactcat tagctttaac tcctttatca ttattttcta gtatgctttt
     7261 ttctccgttt tctgtaatta aattatatac aattacagta agtatacctt ctgctattgc
     7321 tattggtatt tgagtcataa agaaaattcc taaaaattta attgctgaag ccattacccc
     7381 acctgatggg tctgggaaag ccagtgctaa ttgtattgaa gttattgtat atgtggctaa
     7441 gtctcctatt gctgctgcaa gaaatactgg aatagcatta tttccatctt ttttctttaa
     7501 agccttaaat attaggaatg atacgattgg ccctattatc gccattgaaa aagcattagc
     7561 tcctaaagta gttattcctc catgtgctaa taacaatgct tggaatatta aaactatagt
     7621 tccaagaaca aacattacgc ttggtccaaa cattattgct ccaagaccta caccagtagg
     7681 atgtgaacaa cttcctgtta ctgaaggtat ttttagtgct gataatacaa acacaaatgc
     7741 tccacataaa gctaataata ctttcttttt agggtcttgc tttactattt tacctattga
     7801 ttttaatccg aataagaaaa atggtataaa tataactccc catgctatag accattttac
     7861 tggtaagtaa ccttccatta tatgcattgc atttgatgta actggagtta aaatgattaa
     7921 cataagtgca gctataaccc ctaatttaat attctgtttc atcttagtat ccctctctcc
     7981 ctttctaata attaaaacat aataatacat gtctgattta tttaataaaa taaaaaagcc
     8041 taactcgatg gttaggcttc taattttcca caatctaaat acaaacacat aaaacaaaca
     8101 tatctgtata attattataa gaaactttct ccatccccga agaaattact ttaatatttt
     8161 aggcaggtct cctgacttag cttcatccta ctccttttcc ttcccatgag atataaaaaa
     8221 tatcatcata gtggctaaca aaagtttcgt ctgcatcaca gtagcggggg ctgtaaagga
     8281 atttaacctt tttccctatt aatcttgctt aaagaaccta taatattata tatttaattt
     8341 ttaaattatt cttaaatcat tttctatata aatcattgta taccttttct gtgaaaattt
     8401 ctacattttt tataaatttt attcattttt atatttttat gtatataaat tcttttttgt
     8461 aagtttatta atttctaatt atcttcttta aatacattga aactacatca gaaaatctag
     8521 ttatatctct tgaatatata aaatcatttc catatattaa tctttcagct tcaagttctg
     8581 attcttttcc tgtaaatact cctaatacta atatattttg ctttctagct tttctcactt
     8641 cattggctgt atctctcaaa ccgactatac ccctataagc cttttctcct cttaatgttc
     8701 tctcactact ttttcctatc tttacatcat ttggtcttcc atcgctgaga actatcagga
     8761 ttttattttc ttcatctcta tttattagac cactacatat agattttatt gccaaaccat
     8821 ctctattatt accagcacaa aaatattcaa atatattttc actatttttt atactatcat
     8881 cataatcttt aaatcttttt aatattgtat agtctaaaaa actagaaaat cccatcactc
     8941 tatttggtat gccaacttct gttagagcat ttgcaattat gtaagcttga attgccacat
     9001 ttccctgatt tctactttga gagccacttg catccaataa tatatcaata acgtatttcc
     9061 ctttttcatt ttctatatct ttataaaata ttttattatt attacttcta ccaactctcc
     9121 aagctctatt ggctaatata gtaccacaat cagaatatac tctactttta gcattttctt
     9181 ctattaaaat tcttgacaga ctttctttta atctctttat ttgctgtcta tacattctag
     9241 catgttctct aaattttcct aaattatttt ctttttgtct tgttacatat tttagttgaa
     9301 atacattatt acactcactt ctaagaactc catctgtaaa gtgtattcta caaccttcat
     9361 gaacatttct acaattttgt gtctcaattt ttttaatttc acctttactc aaataagttt
     9421 ttccataata atgttctatt tttgaatata tcttatttgc agtgtcttca tcaacatata
     9481 taactcggtc tgctgaattg ctacatttag aatcatcaaa atcccctaca ccttctacaa
     9541 gcatggatga agatattgaa tctatatcac tttcaataat attttcttct tcatcactat
     9601 aaagttcttc atacatgaaa tcagcaaatg tatcaaaatt tacttctaca ctttctacat
     9661 tattttcatc aataagacta ttttcttctc catttaatat gtattcaaaa taagttaaat
     9721 atataacatc taatgctttt aaaatctcca taatatcttg attggtatct atatttctta
     9781 tatcattaac tattcttcta gtcatactat ctaatgctgg gtgcttgtcc atactataaa
     9841 gtacaaatgt gtatttaatt ttatccataa tattatcatt gtgtattttg gtaaaacgtg
     9901 aaacaatatc tttataagca tttcttctta tgtcttctat ccctggcctg tctttaatga
     9961 ccttttcttc aactattgta tttaatataa cttgaattag acctagtata acatctttgt
    10021 tgtatccttt tcttattcta tttgtaagat attttttaac tatatcccaa tctacatact
    10081 ttcttctagc acctgcaatt attgcgaaat atagacctat ttcttttgaa gagtaatctt
    10141 tttctgatat atctatactt tcattgtaat caccagctat tgtccatgat aggttattag
    10201 ctctattttc aaattcataa tcatcatata tccatttcat aatctcacct atattaaata
    10261 tgacttattg gaaacacatc tatactctcc catttattag gtattctagt tttaattaca
    10321 tctccaacca tttctttctc atatacatca aaagtctttc ctgtaagtcc catattaatt
    10381 gcaagagttg gttttaatcc tcttcttata gtcttaagag atgccataag accacgtaaa
    10441 tcaatagctt tactagatat ctctccattt tgagacttca attgtaaatc taaaaatatt
    10501 cctgcaaagt gtattaattt ttcttcatca gcatctgaaa attcattttt taaaatcatc
    10561 attaacttat cttcttctat aggaggaata tctattacca taaatctaga taccagtgct
    10621 tcattaagtt cttttgttcc tgcatattca taattcatag tcccaataaa tcttgttgct
    10681 ggatgtagat tgactctctc atacccaggt acatcaatta ttcttctata gtctaaagct
    10741 gaatgtacca caactatagc atcatttttt gccatattaa tttcatcaaa taccccaaaa
    10801 cctccattaa tagcacactc ataaactgag cctcttctaa gtttaacttc attgtctata
    10861 aatgtatctg tacctataag tgttgaactg tctgtattta tatgaaatga tgtattccac
    10921 tgaggtctat taaatatttc ccctagatta tcagcaagta agttttttcc tgttgccttt
    10981 ggtcccgata ataatatatt ttcttcttct aaaatagctg ctatacacat agacaagata
    11041 tctttaccat aaaaataagt tttagacttt gttactctgt attctaaatc tttttctaat
    11101 ccataataat ttctaaaata cagtacatct tcaataagtt tttcatctac cccttgcacc
    11161 cttagattat ctattatatc catattttta ccccttttat ataaatagta tttgtttcat
    11221 ttcttatcac cattttagca taaaaggata taagctaaaa ttgatttttt aatctcccca
    11281 ttttaagtaa aaccatgcac cattttatca ctacaaaatt aagagtacta cttactgata
    11341 tttcttgatt ttgttcgtct atacgtatat aatatatgct gataaattat gaggaaagga
    11401 tgattgaaat atggaatata tgtcttgccc agaagtagca aaaaaatggg gtatttctga
    11461 aagaagagta caaaaactat gtgaagaaag tcgcatacca ggagtttcaa aactcgggta
    11521 tatgtggctg ataccaaaga acgcagaaaa gcctgttgat ggtagaacaa aaagaagaaa
    11581 ggaagtacaa tatgaatagc gttttgatta tagatgatga caaagaactt tgtgctctta
    11641 tgaaaaaatg tgttgagcaa gaaaatttat ctgctattac tgcacattgt ggtgcagaag
    11701 gtctacattt ggtcgataaa aatagacaca cactctctct tgtaattttg gacataatga
    11761 tgccagatat agacggtttt caagttctga aaaaaattag agaaacaagt aatattccag
    11821 taattatgct tactgctaaa aatgacgagg atgataaagt ttccggacta cgtttaggtg
    11881 ctgacgatta cctaacaaaa ccgttcagca taaacgaact tatggctcgt gtcaattctc
    11941 ttattcgtcg gtatactatg ttaaaccctg cttttataac agacacagat tgcataataa
    12001 taaatggcat gattattgac aagacaaacc gaattgtttc tgtcaataat attcctgttc
    12061 aacttacaag caaagaattt gatttacttg cttttctggc ttctaataag ggacgggtgt
    12121 ttaccaaaaa acagatttat acacaagtat gggaagaaga atatgctttt gacgatagta
    12181 acatcatgtc tttcatcagt aaattgagaa aaaagattga accagacccc aaccatccat
    12241 tttatatttt gacagttcgt ggtgtcggtt atcgttttaa taaggaggct taacatgaat
    12301 acaagtgtct atctgctttt gatattggtt cttagcctga ttattatagc gtaccttgtt
    12361 tctgtcctgc tacgtgtccg aatacaactt acacttataa aagatgcttt agaggatata
    12421 aaaaatggaa accttaatcg acgtatttta acagatgaaa acgacattac aaaacaaatt
    12481 tgctatgata ttaacgaaat agcaatcaat agccaatttc aacttattca gcaaaaacaa
    12541 tcagaacaag cctacaaaca gctaatgaca agtctctccc atgatgtaaa aactccactt
    12601 gcttctttgg tcggttattt ggaagcagta gaaagtaaaa ttgttgtagg agaggaaaaa
    12661 gacgagtata tccatgttgc gtctaataag gctcattact taaagaattt tgtagaaaat
    12721 ctatttgaat gggtaaaatt ggattctaag gaacaagttt ttcattttga tatttttgac
    12781 ttgaatgaat tatcccgtaa tattatatct gattggatac ctgttttaga aagcagtcat
    12841 tttgaatatg aatttgatat accagaaata gagtacttct tacgtataga cgcaaatgca
    12901 tatacccgta tcattaataa cctattacaa aatattatta cccatagtag tggcaacaaa
    12961 atgacattga gaatttttga aaacaaagag caagctcaaa ttataataac agataatggg
    13021 aaaggtatat cttcagacaa tctcccacat atttttgaaa ggttgtacca atgcgaccat
    13081 tcacgagctt caaagggaaa tggtttaggc ttggcgattg caaaggaact tattaacgct
    13141 cataagggaa atattaccgc caacagcact cccggtatgg gaacagaatt tacaatattg
    13201 cttcctaaag ccctgtaaaa gtacgaggct ttcttactct aaaacaagat aaaagcaaga
    13261 ttatggcaag gttttggcaa ggttcatgtt ttataattct aattgtagtt gattttgacg
    13321 ttgtatcaac tatcttttgt agaaacaaat ttattaaaag tgaggaaatt atatatgaat
    13381 gattttgtga ttgaaacaaa gcaacttaca aaaatttatg ggaatcaaac agtagttgac
    13441 aaagtcaatc tccatgtaaa aaaaggccga atatatggtc tgttaggacg caacggagct
    13501 gggaaaacca caattatgaa aatgatttta ggattgactt caatcacgta tggcgaggta
    13561 gatgtatttg gagagaatat taagggacgt gaaaaacaag tatatccccg tatcggtgca
    13621 attatcgaaa cgccaggatt ttatcctaat ctgacaggaa cggagaactt ggagattttt
    13681 gcaaaactgc gtggtacagc cgcccctaat tctgtaaaaa aagcattgga gattgtaggc
    13741 ttaccttata aaaataaaaa gctgtttagt aaatattcac ttggcatgaa acagcgactt
    13801 ggtattgcca acgctatcct gcatgacccg gaactgttag ttttagatga gccaaccaat
    13861 ggtcttgacc ctattggtat tgcggagatg agaaagttta ttagggactt gagcgttgag
    13921 catggaaaga caattcttat ttccagtcat attctttcag aaatctcatt gttggctgat
    13981 gatattggaa ttatcgacca cggtgttctt ttggaagaaa gtagtatgag tgaactagaa
    14041 aagaaaaaca gtaaatatat cctgctacaa gtttcagata ttccgaagac atcacttatt
    14101 ttagagcgtc aatttaacac gaaggattat tccgttcaag atgaccacac tttacgcctt
    14161 tataacacta ctctggatat gggtgaaatc aataaagcac ttgtaatgca gaatgtaact
    14221 gttattagtt ctcaactttg taatgatact ttagaggact actttaaaaa aatcacagga
    14281 ggagagggca ttgcttaaac ttattcaaac agaatttctt aaactgcgtc gtaggaaact
    14341 tatttggctg atgttgttgt ctgctctcat tatgccgttt ttcgcactac tatacttcaa
    14401 ttattttggt aaaacaggtg ttgagccaat aaagttttat aaatggtctg catttggtta
    14461 tacactttgg attattttgc ccgttgtttt gggtatattt tgcaccatgc ttatacacga
    14521 agaaaaccag tatgatatgc tcaaacagct ttggattgta cctattagta aaatgggata
    14581 cttttttagt aaatttttcg ttgttttgat ttactctatt tgttttatgc tgattacagc
    14641 cgttgcttct gtgttattta gtgtactttc tggatatgtc gtatttagct gggcaagcat
    14701 tttatatttg ttgaaaaaat gtttagaaat tggtgtaatc actgcttttg taatgttgcc
    14761 gattttggca atcgctacat ctcaaaaagg gtatattctc ccaatttgta tcacattggt
    14821 atatgccttt tccggttttg ttataatgac aataaatatg tatttacacc cattatccag
    14881 tatgggagtt attatcatga gaaataaaga cattccaggt cttatatttt cacaggaaat
    14941 taatgtccca ttagcatttc tttgtatttg tatttggagt attgtatcag tattgtttgc
    15001 aaacattgca ctaggaagaa gaaagtgagg tgtagataat gtggaaatta ctttgggctg
    15061 aatgtcaaaa aattcgtcgt tcaaaaattg tttggattac agtttttgca tccattatga
    15121 tagcagtcat agtttttgca ggagggcagg atgtatataa tgacccagat ttacattatg
    15181 gcttaaagag cgtttatgac ggttcacggt atatagacaa tgcaggttgg tatatggatg
    15241 cagtacaacc ttggtcaact ttttttgttc ttcctgctgt cattgcccta ttaggaagct
    15301 atattatttg tcgtgaagaa gaggaggaca ctattaaatc cctgcgtctt attcccataa
    15361 atgaaacaaa attaaccata gcaaaaatga ttattacttt tatattcagt atacttcttt
    15421 atttgttact ctttactata acatttttga ctgaatccgt tttacatttt tctgatttat
    15481 cgacaaaact ggttttaagt tgcatgaaag agtattttct tgatggcata ggtgtattct
    15541 ttgcaatttc tcccattatt gccttggtat cgcgcatgaa aaaaggatat tggcttgcat
    15601 tagtattcac agaagtttat tctattgctg gactatttgc gggtatgtca aatgtcttac
    15661 aaaccttttt tcctattgcg gcaatattta atatttctgg atactatata actactggag
    15721 aaaaaattat ggggagtatc ataagtttat tactttgtgc ctgcctttct gcatttatac
    15781 tgaaagggct aaaacacaat gaaaaaaatt gaaatatgta tatcaaaatg aaaaagataa
    15841 aaaaactgat tattctaatt gtattcttgt ttctttttgg catgttacac tgttatactg
    15901 aatggaaatt caacaatgac ccacctaaag gggtaataga aagaataaat gagtggactg
    15961 ataagattgg actggattag taacttatgt taattatagt gaatgtaata ttagcaagtt
    16021 attgtttcgt attttaatac tataacttta aatatttggt tttaatcttc caaacgaata
    16081 taaaaaaact gtcacagagg caacatattt ttgaatattt ataaagtggt agttttgatt
    16141 ttcaaagctg ccgccttttc ttctatataa gcaaacttat cctcaacaaa gtgtaaaaca
    16201 tggtacattt taagaaaaaa gtaccacaga cacaagcaat aaaagaaacg ccgagaacct
    16261 acaaaaacgt agtgcttatg cagatttacg acgccttcta ttttctcaac cgtcttaatg
    16321 accttgtaca aaagaaattt ttctaatatt ttttataatc ttatcttgtg atgtttttta
    16381 tgtataaatg acttaataac aaatccaata aaaattatgg atattggtat aattagaacg
    16441 aggaatttat aatcattata atgtgacatt attcttaccc aactctcaga aaaaatatat
    16501 cctatactga ttaatgaagt attccatatg gttattccca cgatagatga tattacaaaa
    16561 gttataatat tttgacctgc tatacctgct ataaaagata tgtatgttct acataaaggg
    16621 attaatctac atacacaaac agaaatagaa gagtatttat taaaataatc ttgagatgca
    16681 aatattcctt tctttgcctt tggacatatc tctattattt tatttatgat agatttacct
    16741 ccccaatttc ccactgtata acaaaatatc gaaccaagta tacctgcaat tatacttaaa
    16801 attaaaattg tcagaaatcc aaaatgattt cttgcagcaa ttgctccaca taaaggtaaa
    16861 acaacttcgc taggtaatgg aaaacaagca tattcaatca taattaatac aaatacagag
    16921 attaatccat attgtaaaat aaaactattg attacagaca cttacttatc tatctccttt
    16981 cttatttaaa ggttttaagg gtacatgttc taaggattct caccctatat ttaaagaatc
    17041 aatctcttta aactattata tgtatttaca caaaacattt ataacatact ttaaaaatag
    17101 taccttaaaa taagaaactt aataaataaa aaaagattac gtggctacgt cctactctcc
    17161 caggcagttt cccaccaagt accatcagcg ctagagagct taacttctgt gttcggaatg
    17221 ggaacaggtg tatcctctct gctattgtaa ccacataatc tttatgctta atatttctat
    17281 taagttttta ataagtttat agaacctag
//
LOCUS       DAEMHZ010000083        17229 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000083 DAEMHZ010000000
VERSION     DAEMHZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.083"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            142..1026
                     /locus_tag="KPK72_002499"
     CDS             142..1026
                     /locus_tag="KPK72_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2489703.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            1388..2005
                     /locus_tag="KPK72_002500"
     CDS             1388..2005
                     /locus_tag="KPK72_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG2489704.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            2113..4644
                     /locus_tag="KPK72_002501"
     CDS             2113..4644
                     /locus_tag="KPK72_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2489705.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDVKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            5078..5779
                     /locus_tag="KPK72_002502"
     CDS             5078..5779
                     /locus_tag="KPK72_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2489706.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            5829..6911
                     /locus_tag="KPK72_002503"
     CDS             5829..6911
                     /locus_tag="KPK72_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2489707.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFIILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            7172..8272
                     /gene="vanG"
                     /locus_tag="KPK72_002504"
     CDS             7172..8272
                     /gene="vanG"
                     /locus_tag="KPK72_002504"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG2489708.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            8269..9075
                     /locus_tag="KPK72_002505"
     CDS             8269..9075
                     /locus_tag="KPK72_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2489709.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            9093..11231
                     /gene="vanT"
                     /locus_tag="KPK72_002506"
     CDS             9093..11231
                     /gene="vanT"
                     /locus_tag="KPK72_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2489710.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            11545..12165
                     /locus_tag="KPK72_002507"
     CDS             11545..12165
                     /locus_tag="KPK72_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2489711.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(12331..12867)
                     /locus_tag="KPK72_002508"
     CDS             complement(12331..12867)
                     /locus_tag="KPK72_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2489712.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(12980..13654)
                     /locus_tag="KPK72_002509"
     CDS             complement(12980..13654)
                     /locus_tag="KPK72_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG2489713.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            13857..15731
                     /gene="asnB"
                     /locus_tag="KPK72_002510"
     CDS             13857..15731
                     /gene="asnB"
                     /locus_tag="KPK72_002510"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2489714.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(15823..>17229)
                     /locus_tag="KPK72_002511"
     CDS             complement(15823..>17229)
                     /locus_tag="KPK72_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2489715.1"
                     /translation="SIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRYIGVDDSIDKY
                     IEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSKFNSWDLYNID
                     FPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWMDYFLPKLFYN
                     KVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYPECSKFSTLFE
                     LSRKTPNILEIPFERHITNINYSENTNKTSPTTNRPNSKNTLNNTNLTSKKIVQDEEV
                     ELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVVNDGYSFLVRF
                     ENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIVAIGSPLGLFN
                     TVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAGYDDGQNLNLA
                     VESNLVKIFANNFIEIVN"
BASE COUNT         6568 a         1856 c         2910 g         5895 t
ORIGIN      
        1 aatagtttgc attttcctaa tatgtataat tggaatataa atcttgttta ctatgtacaa
       61 atggtattgt attatattaa atagaaaata agaactttaa actataatat tggttctaaa
      121 atataaggta aggagttttt tatggatacg acgaaaatag atgatataag taagttagat
      181 ttaaataatt tagacattaa taagatggat atgggaggta taatggaaag tctattagaa
      241 tgggctacta caagcggagt aaaacttata ataggcatac ttatactttc tattggattt
      301 aaaataataa aaaaatttgt aaaccatgtt atgttattac ttagtaaaag agatattgat
      361 ttaactttaa gaaagttttt aaagtctctt cttttgagtg tacttaaagt tgccgttata
      421 atcattgttc tggaatattg gggaatgagt ttgtccagtt ttgctgctgt gatagcatca
      481 gcaggtgtag caataggttt ggctttacaa ggtagtctat caaattttgc tggaggattt
      541 attatactat tgattagacc ttttaaagta ggagattacg tagaagcagc aggccatgga
      601 ggaacggttg aacaaatagg acttttttat acacaattgg ttactccaga taataaacag
      661 atacttatac cgaatggttc tgtatcaaat gatagtctaa taaactattc agcaaagaat
      721 actagacgag ttgatttgac ttttagtgta gggtatgaag atgatatact acatgttaga
      781 agagtactta aagatattgt taataatcat aaattaataa taaatgaacc agaaccattt
      841 ataggagtag tagaacatgg agataatgct attaaatttg caactagagt ttggtgcaag
      901 acagaagatt attggacaat ttatcttgat ttactagaag aggttaaagt taggtttgac
      961 gaagaaggaa ttacgattcc atatcctaaa atggatttaa cggtaaaaga attaaacaaa
     1021 atttaatttt ttaatatagt gtttaaattt aagaatatca tatagatata cttataaggt
     1081 ttgtaaaaaa ttaataagta ctgttatttt gtaagtaaaa atataaaaaa atgggagtgt
     1141 ctcaaaatga actttttagt tcatgaggca ctctttttgt acgaaataaa atatattttt
     1201 attatttcaa caatgaaaaa gaggcttgtt tccattttga gacagtataa tttttatgta
     1261 actttaattt tagtaaatat aaagttttaa aattgataaa ataggaggaa tttttaatat
     1321 aaaagggtaa ataaagggtt tagagggcat aatatagaat aaattaatat atttaaggag
     1381 ggtttttatg tgtaaaaaaa cgtatttatt agtggtaagt atgcttatag cgggtttatt
     1441 aactgcatgt agtgggagtc caactaagga ttcaggacaa gcacaagata atacaaaaaa
     1501 ggaggcaaat gcatcaaata acgctttaaa agatgaaaaa aataatgaaa atttaatgga
     1561 acaagatttt aaagtgccat acacagatgc aataaacata tttaaagata aatataagga
     1621 tgctgatata gtagatttaa gtcttgagag agatttaaat aaatttgttt atactgtgga
     1681 aggtgtagat gataataatg aatataagat gaaaattgat gctaatacaa aagatgtatt
     1741 agaagataaa acagaaaagt tggattcaga agatttaaat ggagttgcta gaaaagaaaa
     1801 attagattta aatgacataa tgacacctca acaagctatg gaaattgcat taaaagaaca
     1861 aaatggaata gttaaagaat ggagtttaga caaagattta gatgttactt tttataaaat
     1921 aagaatagat aaagataaaa atgaatatga cataaaagta gattctaaaa agggaactgt
     1981 attgaaggta gaaaaagaag attagaagat ttaaactatc tttaaaataa tttctttttt
     2041 gaataaattt tgtttaaaat ataacaagca gggtataaag agactaaata actattttag
     2101 gaggagtacg gtatgtcatt gaaatcacta aaaataaaag aaaatttata ttgggtaggt
     2161 tcacttgacc ctgatttaag agtatttgac ataattatgt atacacctta tggaactact
     2221 tataattcat atgtattaaa aggaacagaa aaaacagttt tatttgagac agttaaagat
     2281 aagcattttg acaattatat tgaaagatta aatgatttaa atatagattt tgaaaaaata
     2341 gactatattg tagtaagtca tactgaacca gaccatgcag gaagtgttga aaagcttttg
     2401 gatttagcta aaaatgcaaa agtagtagct tcagaaacag caattaaata tcttaaggaa
     2461 atcgtaaaca aagactttga atatgttgca gttacagatg gagatacatt atcaattggg
     2521 gacaaaactt tagaattttt ctctgtacct atgcttcatt ggccagatac tatatacaca
     2581 tatataaaag aggataaaac tcttgtaaca tgtgattcat ttggtagtca ctatagtaat
     2641 gataagatag taaatacact tgatgaaaat gaagaaaagg attacttgga tgctttaaga
     2701 tactattatg attgtataat gggtccattt aaaccatcta tggtaactgc tatagaaaag
     2761 ataaaagatt tagatataga tactgtatgt ccaggtcatg gtccagtatt aactgaaaat
     2821 cctagaaaaa ttatagacct ttattacaac tggagtgtaa atgaacaaat aaagttagaa
     2881 aaagaagtta caatttgtta tgtttctgca catggatata caaagattat ggcagaagct
     2941 ataaaagcat acattgaaaa aaatagcaac tataaagtaa atttatttga tgtaatagag
     3001 cataagcaag aagatatttt ggcaaaaata gcagtatcac aaggtgtatt atttggaaca
     3061 ccaacaatat taggagatgc gttaaagcct atatgggata tattaatatc gttaaaccca
     3121 gttcttcatg gtggaaaagt tgcttcagta tttggttcat atggatggag tggagaaggt
     3181 atagaaaatg ctatggagag aataagtcag cttagaatga cagcagtaaa accttttgct
     3241 gttaacttta aaccatcaaa tgaagagatt gataaattac attcttatac aggtaaattc
     3301 ctagataagt taaattctac ttttggaagt aaaaagaaaa ctaagaaatt taaatgtgta
     3361 atttgtaatg aagtttttga aggtgatagt gctcctagtg tatgccctgt atgtggagct
     3421 aaggaagacc aatttataga agttgaagaa gatgaagtta cttttagaaa agatactgat
     3481 gaatatttcg taatagtagg aaatggagca gctggttttt atgcagcaga tgctataaga
     3541 aaaagaaata aaacttgtaa gataactatg atttctaatg aagatgagtt gacttactat
     3601 agacctgcac tttctgatgg tataaatgaa gaacttggtt cagattttta catggaagat
     3661 aaagattggt atgataaaaa taatatagta gtaatattag gaacgaatgt agataaatta
     3721 gatgaagtga ataaaactat tatagtgaat gatggagcta ttaaatttga taagcttgta
     3781 atagctacag gaagtagaaa ctttatacca ccaattaagg gtcatgattt agaaaatgtg
     3841 tttactctta gaaatataaa agatttatat agtgtaaaag aagcactaga aaaatctaag
     3901 aaagttgtag tgattggtgg cggtttattg ggacttgaag ctgcatggga atttagacta
     3961 aaaggattag aagtagtagt agttgaagcc atggatagca tactatcaaa acaacttgat
     4021 aaagaaggta gtaaaatatt agaacaatgt gtaagagata caggaataga tgtaagattg
     4081 ggtgtagcag ttgatggtat tgaaggtgat gtaaaagctc aaaaagttgt atttaaagat
     4141 ggagatagtg tcgactgtga tatggtagta ttttcaattg gtgtaagagc aaacactcaa
     4201 atggttcagg atacatctgt aaaaattgat agaggtattg ttgtagataa gacattacag
     4261 acaaatgtta aagatatata tgcttgtgga gatgttgcac aagtgggaaa tattagttta
     4321 gcaatatggc catcttcagt agaaatggga aaaatagcag gggcaaatgc aagtggagat
     4381 aatttgacat ttgaaagtga agtatatcca gtttcattag atgcaatgaa tgtaaaagta
     4441 tttagtattg gtaatataca aaattttgat aaagaaattt catcaaaaga tgaaggtcaa
     4501 agaatatata aaaaattatt tatgaaagat ggttcattag ttggagctat acttataaat
     4561 gatttaagct gtactgttaa attgataaga ttaatatctg aaaaaggtga ttttgaggat
     4621 attatgaagg ctgatatact ataatttaaa tacattaaaa taaaaaggag ttgtctcaaa
     4681 attgattttt ttctaatcaa attttaaatt caactccttt tttgaatttt tatataaaat
     4741 aacatggaaa ttttagtaat gtctgtggtt acatacatag gtttaattgt tttttatatg
     4801 gattgtctct ggtataataa attgaattgt gtttgactga tagatatttt tattgaaagt
     4861 taatacctgt tatgcgataa taaggacgtg ttaggggagt ttttataata acaattaatc
     4921 tcagtttttg tgttccaatg gtagcatcaa cacattctga tgtagtagtt cttttcttat
     4981 aaaaattagt tacaaatatg cttgtaatta tttgatatgt aaatttacca atattataaa
     5041 tgatgacctc gataataagg agagtgaaaa ataatctatg aacgaaaata ttttgattgt
     5101 ggatgatgaa aaagaaatag cagatttaat tgaagtatat ttaaaaaatg aaggttttac
     5161 agtatacaag ttttatacag gtaaggaagc tctagaatgt attgaatcta aaaatttgga
     5221 tatggctatt ttagatatta tgcttcctga tattgatgga tttcatattt gccagaaaat
     5281 tcgagaaaaa tactattatc ctatcattat gttaacagct aaaattgaag atatagacaa
     5341 gataatgggt ttaaccatag gtgctgatga ttatataaca aagccattta accctttgga
     5401 agttgttgca agagtaaaga cacaattaag aagatatatg cgatataata attcttatga
     5461 acaacagtcg ataattgtta atgagtatga tattaaagga ttgattatca ataaagaaac
     5521 acataaatgt agtttatttg ggaaagaagt agcattaaca ccgattgaat tttctattct
     5581 ttggtattta tgtgaacatc aaggaaaagt ggttccttct gaagaattgt ttgaagctgt
     5641 atggggagaa aaatatcttg ataataataa cacagttatg gctcatatag ggcgattacg
     5701 tgaaaaaatt catgaacctt ctaaaaaccc gaaatttata aaaactgtat ggggagtagg
     5761 ttatacaatt gaaaagtaaa aatattaaag tcaatcctga ctttactcaa gtgagaaata
     5821 aaatttttat gagaacagct ttaatggcat ttatagccat tattttcata tggattttat
     5881 attcttttat attgtatggt aagttttcta tctggatagt atcattcttt gaaaatgctt
     5941 ttcgtatgga atataaagct gcattgaatt tatatcagca gattttccgt aatagcatgg
     6001 agctcttcat aatactagct atggtgattg tgttttttat aatattccgt gtttatttaa
     6061 attggtttac aaaatatttt agtgaaataa atcaaggtat tgactctctt atcaaagaag
     6121 atgttggaga agtggcttta tctccagagt tattggcaat agaaaagaaa ataaactcaa
     6181 ttaaacatat acttgaacaa cgtaaatttg agacacaaat ggcagaacaa cgtaagaatg
     6241 aacttattgt ttatcttgca catgatttaa aaacaccact tacatcagtt atagggtatc
     6301 tgacactact tcgagatgaa agtcagattt ctgaagaact gcgaaaaaaa tatttatcta
     6361 tttcgttgga taaggcagag cgtcttgaag atttaattaa tgaatttttt gaaattacaa
     6421 ggtttaattt atcaaatata tcacttgaat acagtacagt aagcttaact cgtatgcttg
     6481 aacaactaac atatgagttt aaacctactt tgatgagtaa gaatttaaaa tatacactga
     6541 atgttacacc aaatatgatg ataaggtgtg atgttaataa aatgcaacgt gtatttgata
     6601 acttattacg aaatgctgtt tattatagtt ttgaagatac tactattgaa ataacagctg
     6661 tacaggagga tgattatata aagcttaaat ttataaatca tagcaatacg attccggagg
     6721 aaaaacttga gcgtattttt gaacaatttt acaggttgga tacatctcgt aattcaaaca
     6781 gtggtagagc tgggttggga cttgctatag ctaaagaaat agtggagctt cataacggga
     6841 caataacagc tcatagcgag aacaatataa ttacctttaa ggttattatt ccgattgtag
     6901 gaaatttgta agaatttaag tataaaaaaa taggaaattc tttaggataa ataaataaag
     6961 agcactcttg ttattgatat aattactgta tcaaaacaag agtgctttta ttttgataca
     7021 gtaattatat caatagaaaa atgtgaaaat atattatgtg gaacagatgc aattgtaagt
     7081 gaaggttgat tggatttgaa atagaaaaag tattttaaca tgtttctgta gattgtataa
     7141 aaataaataa atcaacaaag gagaatcaaa gatgaataat aaaaaaattg cagttatatt
     7201 tggtggaaac tcaacagaat atgatgtatc attacaatca gcttttgcag tacttgagaa
     7261 tttgaatacg gaaaaatatg atattatacc aattggaatt acaagggagg gtaattggta
     7321 tcattatatt ggagattata ataatatcca gaataacact tggtttgaaa gggatactga
     7381 tataattcca gttattgttt cgcagaaccg tgtcaaaaat ggtattattg aatttttaat
     7441 tcctgatgct gtttctcaga atcatattcc aaatagtgct attgaatcat gtgaaaatga
     7501 tattaaatac actaaacttg attttgtgtt tcctgtactg catggaaaaa atggagaaga
     7561 tggaacagtg caggggttat ttgaattggc aggtatacag gttattggat gtggtactct
     7621 ttcttcagca ttatgtatgg acaaagacaa atcacataaa cttgttcatt cagaaggtat
     7681 taaagtacca aaatcaatat tactaaaata ctcagataaa tttaatgtgg ctgaactgtc
     7741 aaaagactta acttatccat tatttataaa acctgtgcgt gcaggttcat catttggtat
     7801 tagtaaaatt tataaaaaag aagagttaca agtagcagta gacttggctt ttgaacatga
     7861 taatgaagta ataattgagg aaaatattga tggttttgag gtagggtgtg cagttttagg
     7921 aaacaatgac ttacttgttg gaagagtaga tgaaatagaa ttgtcaaaag gattttttga
     7981 ttatacagaa aaatatacat tgaaaagttc aaaaattcat atgccagcta gaattgattt
     8041 ggattgtgag aaaagaatac aagagacagc aaaaattatt tatagaactc ttggatgttc
     8101 tggatttgct agagtagata tgtttttaac tccatctggt gaaattattt ttaatgaagt
     8161 caatacaatt cctggcttta cttctcatag tcgttatcca aatatgatga aaggtattgg
     8221 actgtctttt gaagaaatat tggacaagat aattgaggtg tacataaaat gatgaataat
     8281 attttattag ataaaaataa agaagataaa atgacaaata ctatcttatt gactaaagat
     8341 aaagtttatg atgggaatct aattctagta aatgcttttt taccagttaa aacatctgaa
     8401 gatatagatt taattcctgt agatacaagg tttccaagta ttcttatgaa acgtgaagct
     8461 acaaacattt tacaaaatat tttaaaaagt atatgtggaa taaatgaaat agtacctgta
     8521 agtggatatc gtactgcaga ggaacaacaa gatatatatt caagttctct gcgtgacaat
     8581 ggaaaagact ttactaaaaa gtttgtagca cttccaaatc acagtgaaca tcagacaggg
     8641 cttgctattg atttagggtt aaagaaagaa gttatagatt ttatctgccc tgaatttcct
     8701 tataatggta tttgtaatga gtttcgtaag gcagcactac attatggttt tatagagcgt
     8761 tatcagtgta aaaaagaaga aataacagga atatctcaag aaccatggca ttttcgttat
     8821 gtgggatatc cgcattctga gattatcaat aaaatgaatc tcgctcttga agaatatata
     8881 gaatatatta agagattttc gtatacaaat tgtttaaaaa tagaagataa agaaaaaatt
     8941 atagaaatct tttatgtgcc tattcatact aaagaagaag tgagaatctc tatacctgaa
     9001 caatgtgtat atcaaatatc gggtaacaat aaggatggtt ttattgtgac attttggaga
     9061 aatagaaagg actaagaaat ggaggaacaa gaatgattag gaataaatct tatgcagggc
     9121 ttgattattt taggattata gctgcattac ttataattgc aattcataca tctccattat
     9181 tatcatatag taaaatggct gactttgtgt taactagtat aattgctcgt attgctgtgc
     9241 cattcttttt tatgctatca ggcttttttc taatttcaac atataattat aattgtaata
     9301 aactgaaatc ttttctaaaa aatgtattat ttatttacgg aatagctatt gtaatttata
     9361 ttcccttaaa tatttacact ggatattttt ccatgaataa tttagtgccc aatattatta
     9421 aggacattgt atttgatggg actttatatc atttatggta tctacctgca tcaattattg
     9481 gtgcaagtat tgcatacttt cttgtaaaaa aactaggttt aaagtactca ttagtaatca
     9541 ctattgttat ttatgtaatt ggtatgtttg gtgatagtta ttatggtttt ttagaaaaaa
     9601 taccactatt aaagaattta tatgggtata tttttgaagt atcagattat acacgaaatg
     9661 gtatattttt tgcacctgta tttttcatac ttggtggaat aatatctaat aaatctaatt
     9721 atatgtcaac taaaaatagc ttgattggat ttcttatatc attcttactt atgttatgtg
     9781 agggaatgtt tttacataaa ctaggggtac agcgtcatga tagcatgtat attatgttat
     9841 tgccatgtat gtatttctta tttacagcct taacattttt gaaaggttct cgagctaggt
     9901 tattgcgaac atcatcatta attatttata ttatacatcc tatgatgatt gttgttattc
     9961 gtatgttttc taaaatattc ggaatacaaa ctgtcttggt tgacaatagc cttattcatt
    10021 atatagtggt aagtatagca tcttttatag cttctataat tattatattg attcttaaac
    10081 ctctaaaaaa agacttgaaa ggagaacata aggtaaatac caatagagct tggattgaaa
    10141 ttgatttaaa taatttggaa cataatgtaa agatgataca aaaagctatg ccagaagatt
    10201 gtgaattgat ggcagttgta aaagctaatg cctatggtca tagtgctttt gaagtagcga
    10261 cttgtgcaaa ccaaataggt gtaaaagcat ttgcagtagc aacaatagat gaggggattg
    10321 atttacgtcg atatggaata ctaggagaaa tattgattct tggatatact aacccaataa
    10381 gggcaaagga acttcataaa tacaatctta cacagagtgt tattgacctt aatcatgcta
    10441 tctctttaaa caaacagaaa tacaacataa aatcacacat taaaattgat acaggtatgc
    10501 acagactggg ttttgatgtt aatgatgtga ggtcaatttt aaaggcattt aaattaaaat
    10561 acttagatat atgtggcata tttacacact tgtgtgtttc agatagtctg aaagatgaag
    10621 atgtagactt tacaaataaa caaattgaat gtttttataa gttaattgat tttctgttaa
    10681 aaaaaggtat aaaaattcct aaagtacata ttcaaagtag ttatggtttt tttaattatc
    10741 ccaatcttaa gtgtaattat gtaagaattg gaattgcatt atacgggata ttaagttctc
    10801 caaatgatac gactaaattg caacttgatt tacgacctgt tttatcctta aaatcacaag
    10861 taattcttat tagaaaaata caaaaagatg atagttttgg atatggaaga gtctttattg
    10921 caagcagaga tagcgtaatt gctatattat ctattggcta tgccgatggt atacctagaa
    10981 atttatcctg tggaaaaagt tacgtaataa taaatggttg tagagcagca atagtgggtc
    11041 gaatttgcat ggaccaactt gctgttgata ttacagatat acctaatgta gaagttggta
    11101 atacggcaat aataatagga agagataatt tatctgaatt atctgcttct gaagttgcaa
    11161 ataactcatg tagtatatca aatgaattat tgagcagagt aggaagaagg ttaaatgtta
    11221 taaagaagta actcaattaa atagtaatat ttgttgtact gtgtaattta ttttactctg
    11281 actagacatt tttagtaata ttatgtctaa tatatatttt ttataactta catgacaatt
    11341 taagttataa aaaggtgatg tttcagattt ttattttgaa acatcacctt ttttgttata
    11401 atatataata aaataattat accttaactg taaacttata tagattattt actttttaat
    11461 attagattca ttttttgaga ttggtatata ataaacatat agagtatata ataatagtta
    11521 aagttaaaaa aggggaaaag caatatgcag tatatacaag aactacttga tttgactcaa
    11581 aattattggg tgttagccac aataatcggc ttattaagtg catttataga aagctttata
    11641 ccagccttac cacttgttgc tatagttaca gcaaatgctg caatacaagg attatttatt
    11701 ggatgtttgt tatcatggat tggctcagga cttgggacaa cttcattatt cttattaata
    11761 agcagattta atgatagtaa gctttttaat aagttgagaa atagtaaaac tgaaaaagca
    11821 ataagttgga tggataaaca gggatttaag ttattattta tagcgtatgc ctgtccattt
    11881 atgcccggtt gtttagttac tatcgcatca gcttttggta aaaaagattt aaaagatttt
    11941 gtgccagcaa tgttggcagg aaaatttgta atgtttatag ttataagtta cgtggcaagt
    12001 gatatagaag gttttataac aagtccttta aagatagcat catttatatt attggtgttt
    12061 ttgtcatgga agattggaaa tagagtaaat aaaaatcttg aaaatcataa ttatgatttc
    12121 catcataaaa aacatcacaa tgataaagat gataaaatga tttagcttgt acgaaataaa
    12181 acgtatctta aaatgattta gtactaaatt attttaagat acgttttatt ttttgtatta
    12241 aggtacaaaa tttttagttc ttttgtaaac taaaatatat aaaacttatt tatacttagt
    12301 tcaaaatatt gttatttatt ttttctacaa ctataataaa atataaaatt gatatccaat
    12361 caaaaaaata actatgaaat ttaatagctt agatatgaat ttttctgttt ttgagttagt
    12421 ttttataggt agtaaagaaa tgtttatggt aatgggataa aacaattcaa taccttcttt
    12481 agtaataagg tcaagacaca agtgagatag atatcctatt ataaacccac tggacaaaca
    12541 tgagaaaact atattattgt tagtgaagat tgtcagagat ttaaaaaaac tataaataag
    12601 taataaggca attagactgt gagtaaaccc tctatgtcta aacctacttc caaataattt
    12661 gtatattaac ataaattttt tacttatgta agaacctctc atatctatat ctggaaataa
    12721 agaacctaaa tatgcaaaat aaatataaat tataattaat actattttat atgcaatatc
    12781 gtatttaact aggtatagat tgtttataaa ggggagtatc aaaagtgcga atatataacc
    12841 tcccctttca tgtgtttctt ttatcatata aaaatcctcc tgaatcgtca ctataaacat
    12901 tatattggta aatttagact atcttttatt atattatata gcaaacataa gtttttttca
    12961 aaaggatttt aatatttaat taatcttgag attttaaatt tttgtaattt tctaaagcac
    13021 cattccaatt tactacatta aaccagttgt caatgtattc atttcttcta ttttgatatt
    13081 ttaaatagta agcatgctcc caaacatcaa gtcctattat aggagttagg tttttactta
    13141 cagggctatc ctgatttgga gtagtcataa tagataattt cccatcttta gtagctacaa
    13201 gccaagccca accagaacca aagacatcta aagcagattt ttggaactct tgcttaaatt
    13261 tttcaaaaga accaaagtct ctatcaatag cttcttttaa agattcagaa ggtatggttt
    13321 tttctggtgt cattatatca aaaaagaatt tatgattata agctccacct gcattatttc
    13381 ttacagttgt agcaatatct ttaggtaaag aatctaaatt ttgcaataat tcacataaag
    13441 aataattata aagctcagga tatttttcaa gagcagcatt taatttatca acataagctt
    13501 gataatgctt atcatgatgc agtttcattg tttctttatc tatataaggt tcaagtgcat
    13561 catatgcata aggtaatggt ttaaccttaa atttgttatt ttcaggtgta aaagcaaatg
    13621 aagttatgca ctgtgagatt ataaataaag acataataac tggtattaat atttttttct
    13681 tcataaaaat cacctcctaa tatagtattt ctagaaataa aaaaaatatg aaatattaac
    13741 agataattta taagaattta atagcaattt cattttaaat tgaataagtt ttaaagccaa
    13801 agacaaaact atgaaatata gaataagact aaaggacaga aaggaatttt gattatatgg
    13861 acggcaatat ttcaatttat tataaaggtg atatatctaa aaaagaagat attaaattta
    13921 aaataaaaga atctgtaaat aatacaagac tttctagtag aatagagtat aaggatgagg
    13981 aaaaaacttt tgattttgaa agatttaatg taattgatgc tatttatgga aataaaccat
    14041 ttgaaaaaga tggaaaagtt attgttttta atggaaatat atataactat catgaaataa
    14101 aagaagaatt aattaaaaaa ggacacaatt ttactacaaa tgatgatatt gaagtcttat
    14161 tggcatctta tatagaattt ggtaagaatt gtgtacataa gattaaagga atgttcaact
    14221 ttataatcta tgatagagaa aatgaaagta tatttggagc aagagattta tttgggataa
    14281 aaccacttta ttatataaat aaggaaaatg caataatttt ttcctcagag tataagtttt
    14341 tacttgaata tatgaaaaat ttaaatataa atgaaagaag cttacaaagt tatttttcat
    14401 ttcaatatgt acttcctgaa gatactatga tacaaggaat aaggctgata ccagcaggtc
    14461 actattttag ggtagaaaat ggtatactat ctttaaagag atataataaa cttgagttta
    14521 gaagtagtac aaaatttttt tatactaaaa atcacttagg caatagagat gtgaatgagg
    14581 atgatataag aaatatagta gtagattcaa tagtaactca tatggaagag gagaaagaaa
    14641 ttggaacatt tctgtctggt ggaatagatt catctattat aactactgta gcttcacaaa
    14701 ttaatccaaa tataaaatct ttttctgcag gctttagtgt caaaggatat agtgaattag
    14761 aagtagcaaa gaaaacagct gataagttag gtatagaaaa tatccagatt aatataacac
    14821 aagatgaata tataaaatca ttaccgaatg taatttattc tttggatgac cctatagcag
    14881 acccttcaga agtaggtctt tatttcttaa ttaaagaggc aggtaaacat gttaaagtag
    14941 cattgtctgg agaaggagca gatgaattat ttggaggata taatatatat aaagagtata
    15001 gtactatgaa atcagttgtc aattcaccta cttatattaa aagtatatta ggaagagttt
    15061 cagaacttat gccaaacata aaaggaagaa attatcttta tagagctaca acaccattag
    15121 aaaaaagata tataggaaat gcaaaagttt ttgaaaacag cgaagtaaaa agattctttt
    15181 ttaagtataa ggaaaagaat atatatgaat acctgttgtc taatttgtat agagatgcac
    15241 aaaaaaataa ttacgattat ataagtaaaa tgcaacatat agatgtgaat acatggcttc
    15301 aaggcgatat acttcaaaaa attagcaaac tttctacagc tgagcaggtt gaacttagag
    15361 taccattttt atataaagat gtatttgatg tagcaaaaaa tttaagaatg gagcaaaaaa
    15421 taaataagaa taatacaaaa gttttactta gagaggcgtt tagagaaata gtcccagaac
    15481 atgttgttca aaggaaaaaa ttaggatttc caactccaat aagagtttgg ttaaaagatt
    15541 ctctagggga tattgtaaag gaaactattt ataattcaaa tgtagatgaa tttatagata
    15601 aaaaatatgc aataaaactt ttagatacgc atcttaaagg tcatagagat aattcgagaa
    15661 aaatatggac tatatttaca ttttgtctat ggcatcaatt atttattgag cataaaaatg
    15721 tcgagtatta atactttttt caatattaat cagagggcaa aagcatgcaa tgttttcacc
    15781 ctcctgatta ttatggaata ggataagtag aattttgatt gattagttta ctatctcaat
    15841 aaaattattg gcaaatattt tgacaagatt actttcaaca gctaaattta agttttgtcc
    15901 atcatcatat ccagcggaaa tcaatcctag taagtttcca aacatatcta aaagagctcc
    15961 tcctgaacta ccactagaga ttggagctgt aaattgtatc atttgaaccg tttcaaaatc
    16021 acgaaaagct gaaataattc catctgaaac tgtattaaaa agacctagag ggcttccaat
    16081 tgcaactatt ttttgacctc tcacaggttt tttactgact ttaacaggta ttggcttgca
    16141 ttttctatca acctttatca cagctaaatc ataatctgaa tggtatttta taatttggta
    16201 agatgtataa actttatcat cattttcaaa tctaactaaa aatgaatatc catcattgac
    16261 tacatgaaga ttcgtaagta tataaccttc attatttatt actactccag aaccgctctt
    16321 aaacggttta ttttcgttat tgcacacaac aatcataaca acagaagctg ataactttgc
    16381 aaggtcttca taagaaagtt ctacttcctc atcttgtaca atttttttag atgttagatt
    16441 tgtgttatta agtgtatttt tagagttagg tctattcgta gtaggagaag tcttattggt
    16501 attttcactg taatttatat ttgtaatatg cctttcaaaa ggtatttcta atatatttgg
    16561 agtcttccta gaaagttcaa atagagtaga aaattttgaa cattcaggat ataaatcaag
    16621 aatatcttta tttggattta tatataaaat atcacaccca agttgtgata aaaaatataa
    16681 gaaaaaaagt tcttgccttt taatatttcc aaagtatact atctttggtg aaatagtagt
    16741 cttcaccttg ttataaaata atttaggtag aaaatagtcc atccaacaca aaagtttaac
    16801 tgcaaagttt ttgatgatag aggatgtaca atttggcata cttgtggaaa ataaatctaa
    16861 agtattttta aatgcaatct ttttagtcca atttaaggtg tcattttcta ttttacatgg
    16921 aaagtcgata ttatataaat cccaactgtt aaacttagaa ctattataaa tattaaaaat
    16981 tttaagcatc ttatctgttt gttctttgtc aaaatttatt tttagattgt ttaatctaat
    17041 atagggaatt gtaggattat taaaaaaata agtatctata tttttaattt tttctatgta
    17101 tttatcaatt gaatcatcaa cacctatata tcttgtaaag tagacttctt taatatatga
    17161 agaacgctta tttttatagg tagtatcaga tataaattca attagaggtt cagtagactt
    17221 ttttattga
//
LOCUS       DAEMHZ010000084        17176 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000084 DAEMHZ010000000
VERSION     DAEMHZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.084"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..932
                     /locus_tag="KPK72_002512"
     CDS             57..932
                     /locus_tag="KPK72_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG2489716.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQTLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1289..2587
                     /locus_tag="KPK72_002513"
     CDS             1289..2587
                     /locus_tag="KPK72_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG2489717.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVESIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKVLEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRSIEFYKKPLSSDLNN"
     gene            2777..4168
                     /locus_tag="KPK72_002514"
     CDS             2777..4168
                     /locus_tag="KPK72_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG2489718.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4345..4932
                     /locus_tag="KPK72_002515"
     CDS             4345..4932
                     /locus_tag="KPK72_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489719.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEVNDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4965..5756
                     /locus_tag="KPK72_002516"
     CDS             4965..5756
                     /locus_tag="KPK72_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2489720.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNIDVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMNDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5937..8354)
                     /locus_tag="KPK72_002517"
     CDS             complement(5937..8354)
                     /locus_tag="KPK72_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489721.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGDQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSIVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8367..9032)
                     /locus_tag="KPK72_002518"
     CDS             complement(8367..9032)
                     /locus_tag="KPK72_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489722.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9114..10145)
                     /locus_tag="KPK72_002519"
     CDS             complement(9114..10145)
                     /locus_tag="KPK72_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2489723.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMIKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10135..10821)
                     /locus_tag="KPK72_002520"
     CDS             complement(10135..10821)
                     /locus_tag="KPK72_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489724.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTVKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10979..12043)
                     /locus_tag="KPK72_002521"
     CDS             complement(10979..12043)
                     /locus_tag="KPK72_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG2489725.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            12305..12976
                     /locus_tag="KPK72_002522"
     CDS             12305..12976
                     /locus_tag="KPK72_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489726.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            12978..13886
                     /locus_tag="KPK72_002523"
     CDS             12978..13886
                     /locus_tag="KPK72_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2489727.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            13963..14649
                     /locus_tag="KPK72_002524"
     CDS             13963..14649
                     /locus_tag="KPK72_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489728.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            14646..>17176
                     /locus_tag="KPK72_002525"
     CDS             14646..>17176
                     /locus_tag="KPK72_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2489729.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTESNLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSD"
BASE COUNT         6466 a         2062 c         2556 g         6092 t
ORIGIN      
        1 taaagtttat attaaattta ttttattgat gaaactaatg gggaggttaa aactcaatga
       61 ttacttttga tgtaaatgga gctattatca gatttgatga tacaaaaaat aattataata
      121 agataaggaa aatctttaaa ttatatgctt tagaaatatc taaaaacttt gaaagagatt
      181 gtttaaatag ttttcaaact ctaaagcaaa tatctgatag aggtttaagt ttaggagaaa
      241 aatatataga agagtctttt aagaaaggaa ttgagactat agtaagtttt ggaattataa
      301 ctatagatat cgataccttt aaaagtgttt attgtgaaaa atatttagat tttaagagac
      361 tttttaataa tttgaataaa acttttatta taccaaataa aaataaaaaa aatggccacg
      421 cgagcttctt agaaatagaa tcgacaataa agaaaatgag caattattta tacaatgatt
      481 gttttaaaat tcattatgct gttgtagatg cccttataga aaatcgtata actgcggtac
      541 attcttatat tgatgaagaa tctataaaaa aatctaatgc cttatttaat aattataaag
      601 atggttttat tgggaaggct gacgaatgca aagtagtaaa acaaataata actttaaatc
      661 catataggga agatgtatac gaatttttgg taaaagaaga tggagatttt agtaaggaaa
      721 ttgagagatt gactgaattt ttaggctatg atataagaga atataaagcg tcattaatgg
      781 atatttatat aaatgagtta atggagagcg atgaaaatga tgttgaaaat gcaaaagaaa
      841 agattgaaaa atatgctaga tatattggat gtagtgatga gaatttatat gtagcaagag
      901 ttaatgccat atatactttt gcaaatgcat aattttatta aacattaaat attagatgtt
      961 cgattatata aatttaaata aaaaaatatc cttattaata acaaatagtt tatattaaaa
     1021 accattcttt aaatgagtgg ttttttattt tgaatatata tattctaaac ctaatagcat
     1081 tcctatcttt aattagccaa aaatcaactg gatttcatat agaccataat tttaaattac
     1141 taaaaaagac aatatgttga caaagatgaa atatgttgat aaaatgaaat agtcaatatg
     1201 gtgtcttaat ttaaaaattt atttatcttg tattagataa agctgtttta tataaaacat
     1261 aaataattat tataaggaga aatagattat gaaagtaaat gtatacaatg tagaatcatt
     1321 tgtggatgaa agacttaaga ttatggtttc agacttaatt ccaaatacta agcttaaaat
     1381 aagtgtgaaa atggaatttc catggtgcaa gggagaagaa ttttcatctt atggagtgtt
     1441 ttgttcaaat gaaaaaggag aagtagattt agatttagtt gaatctatag aaggtactta
     1501 taaggctgat aattgtatgg ggttaattta ttcgctggaa aaatctaaaa ctgaaggtaa
     1561 aaattttgca gaaaatatat ctattgataa accaataatt atgaatatga tatttgaaac
     1621 ttctactgaa agaaaagaag ttgaattaaa gagaatattt aaatcagaag atattaccac
     1681 aaaatacata tctgatgagt ttataggaaa attattttat aaacaaaatt ctaataataa
     1741 aacaatttta atgctaggtg gttctgatgg aaatttagac gctctagacc ttttagcagc
     1801 tccacttgca tctagaggat tcaatgtact aacagttgca tactttgctt tagaagggtt
     1861 accagataaa ttagaagagg ttccattaga atattttgaa aaagtatttg agtggattag
     1921 tgaaaatgaa attaccagta ctaaagaaat atttgtacat gggacatcaa agggtggaga
     1981 attggccttt ttacttgctt caagatatga acaaattaaa aaggttgtag tatcacaacc
     2041 tcacatttat tgtttccaag ctcttaatgg attgatgagt ggaaatgata cttcatcatg
     2101 gtcatataaa ggagagccat ttccatatat caaagtagac aatgatatat tctttgaaga
     2161 acagaaaaaa aatatatcaa aaggtattcc atttggattt aatagtactt ataagaaaag
     2221 tcttaaaaga gcagagaata gaaaagaggc tagaataaaa atagaaaatt ccaaagcaga
     2281 tatacttatg attgctggaa aagaagataa catatggaat agttatgaag cttgtttaaa
     2341 ggtattagaa atctttgaag aaaataatta tccatataac gtagaattat taacatacaa
     2401 taatatgggt catccgttac caattccata tattatgcct ctaaaggaga ctttatgtat
     2461 gagtatgagt ggagggattt tttcttcagg tggaactgta gaaggaaatt caaaaggaca
     2521 gtttgaatca tggaatagaa gtattgaatt ttataaaaaa cctttatcaa gtgatttaaa
     2581 taactaatat taagtgattt aagtaactaa aaatgaagtg gtctttctat atatgcacaa
     2641 aatctatcaa ttttatagat ttaatttatt gtttatataa tttgcatgtt atatactaat
     2701 tttaatgaca ttgataattt ataaacaatt ataaagttgt aaacttataa gagttattga
     2761 aaggtggagt gaagttatgg aaagactcgc tagaagggaa caacctcata taaaatttgg
     2821 aatgtttaag ataagaattc catttataca ttacagattt gagaaaccag aagctattca
     2881 aggtataata tcaagtatga caagtttagg cacaattggc cttagtacac aaattttggg
     2941 attaccctat gaagtggcat ggagtatggc tatcattaac tcaatattat actgtttgca
     3001 tgtttttatg ggagacccag ttgtacctgg ctggattaca gcttctataa ctcttacaac
     3061 tgcatatttg ttaaagttca gcatgggcat tgaaagaata caagcattaa cagctttaca
     3121 aatagattta ggaataatat ttattttaat gggtataact ggtgtagctg gaaagttagt
     3181 tagtaagata ccaaattcta ttaaaggtgg aatactaatg ggtgtcggta tatcgactat
     3241 aatatcagag tttaatccta aagggagatt tgattcttat cctatatcta taacagtagg
     3301 aattttggtt gcttgttttg taatgttttc tgaaagattt gatactctaa aagttaagaa
     3361 taaattttta tttcatttag gagaatatgg tgtagtatca gctattttag tgagtttaat
     3421 agtaggtata tttagtaaag aaatagaaat tcctagattt agttttgaca atttagttta
     3481 catagtagat tttaaaaatc tgataaatac agtatctcct tttggaatag gttttccaag
     3541 tgtaatgtta tttatacaag ggataccaat ggcttttatg atatacataa ttgcctttgg
     3601 tgattttata acaggtgaaa acttagtact ttctgaaagt gaaaatagaa aagatgaata
     3661 tatagatttc aattcaaata ggtctaatgt tataagtggg ataagaaata tttttatggc
     3721 aatcttttct ccatatatac caatgtgcgg tccattagct gcgacactta caggaagtgt
     3781 tgctcaaagg tacaaggttg gaaaggaagc catgcagtct gtttttagtg gcatgggtac
     3841 attagtatgg gtttcagcaa tttttatctg tttttatcca attgcgcaga ttgctacacc
     3901 tcttatacca ttagcattgt cagtaacact tttagttcaa ggatatttat gtacaaagct
     3961 atcaatggag ctatgtaata caggtgttga gaagggattg attggactta tgggaggtgt
     4021 aattgctgct aaaggcggaa catggggact tgctgtaggt tttatactgt atttcatttt
     4081 aattgattct aaaaaaagaa aagaaaaaga tattaattca atagaagatt gtgtatatga
     4141 aatattagac aaaaagaaga gtatttaaag gaaaatctga gttgctgtta tctattaata
     4201 gattaattgc aactcatgtt ttatttataa gttttaggaa ttgcaaaaaa atgcaaagct
     4261 ttttcttgac ataatttctt gatatgttac tatagttata taacatatgt gttttataaa
     4321 aatttaatgg agaggtgaac atatatgcca cctaaagtaa agataacaaa agaaatgatt
     4381 ttgaatacag tcttggatat tacaagagaa gtgggatttg aagctgtaaa tgcgaggagt
     4441 attgcaagta aattacagtg ttccactcga ccaattttta cttgttacga aaacatgaat
     4501 gaattaaaaa atgaatttct tgcttttgca tatgaatatt ataatcagta tgttgttaat
     4561 tatcgtaatt ctaaaagtgt aagcccttat ttactgcttc cgctttcata cattgaattt
     4621 gctcaagaag aaacgtattt atttaagttg ttatttataa atgatttaga tttgatgatg
     4681 aaagaggtaa atgattttta taaagaaatt gacaatgaaa aaaaggcaag acttttttcg
     4741 gaaagcattg gaatagattt ggaatgtgca aaagtaatat ttttagattt atttctttat
     4801 actcatggca tagctgtctt aacagcaaca aaaaaactaa cattagatag atatagtgct
     4861 gaaaaaatgt tgaaaaatat attaacagct tttataagac aagaaaaacc agattggaat
     4921 ttgtctattt gatgtttatt aactaatata gaagaggtga aattatggaa acaaaacaat
     4981 acttaattga cttttataat acttatgatg aagatagtcg acttgcatta aaacatggaa
     5041 tggtggaatt tctcactact atgcattaca ttgataagta cataaaatca ggtgattgtg
     5101 tgcttgaaat tggcgctgca actggtcgtt attctcacac actggcacgt cagggatatg
     5161 atgttgacgc agtggaatta gtcgaacata atatagaggt tttccataaa aatactcaat
     5221 caaatgaaaa tataagcatt actcagggta atgctatgga tttatctgtt ttcccagata
     5281 ataaatatga tattacgtta ctgttaggac cactatatca tctttataac aaagaagata
     5341 aacaacaagc tttacatgag gcaattcgtg taacaaaacc tggtggtgtg gtttttgctg
     5401 catatgttat atctgatggc tgtctcattg acgaggggtt ccatagagga aatattgatg
     5461 tatctgaata tattgaaaaa ggtttgattg atccacagac atttgctgct aagtctgaac
     5521 caaaggattt gtttgagctt gtacgtaaag aaaacattga tgatttgatg tctgcattta
     5581 atgtaactag actgcattat gtcgcatcag atggtttggc tttgtatatg cgtgaagcag
     5641 tagactctat gaatgatgat gcatttgcat tatatttaaa atatcacctt gctacttgtg
     5701 aacgtgaaga tttagtaggg gttacaagtc atgcaattga catatttaga aaataaggaa
     5761 tatgcttgtt tatgtctctg gtgatgttgc tcggagatat aaggaagtgt atctgtataa
     5821 aataaaaaca agtataggga tagaaaatta tgtaaagtca aaattttcta tccttttagc
     5881 ttttaaaaaa ttatgttaaa acttgtataa aaaatggcta ttttttatat acattcttac
     5941 tgagaacgta agcgttctgt aagtgatttt ttttctatat ttcttacaag taattcagtc
     6001 actaaaactt gtataccaat taatacgatt gaaaaaatta aaatagtaaa tatagaaaat
     6061 ttaaaattaa taaagtctaa tctaattaca tcttttataa aagtgcaaag tagatttgaa
     6121 ccaaaataac ctagtatact tgaagtacat atagctttga tacttatgtt tagaccttct
     6181 ctataaagca tatttcgcag ttgctttctt gtcattccaa cggcttgaag taaggcaaac
     6241 tctttttttc taattaaaac gctgtttatt agggtattgg tcatatttag tataccaaag
     6301 caagaaatta aaccaacaaa aatatatgaa gctataatta tatattgaaa tgctctagta
     6361 atcattctat aatcatcttc atatgaataa agcattaaat ttgaattttt ctgaattata
     6421 cttaacaact tttcttcaac ttctttagaa tgctttttat ctattaaaat agaaaaatct
     6481 aaatttgtat tatatggtgt tagagattct aaagttttgt ctgaagcaaa aaacatacct
     6541 cctgtattgg aattgtcaat gattcctata attttcattt tctttttaat aatatcattt
     6601 ccactaaaga tattaaatgt aactgtatct ccaactttta aatttaatcc ataatatttt
     6661 gttctatatt tatttataat taaactatct gaatctaaat gtgtatagtc tatttttcca
     6721 ctgacaatat attcttcaag tgattttgaa agttcaggcg taataccttt aattagagta
     6781 ataaattgtt tttcgtctct atctttgatg taaggttcta taatattact tttaattgat
     6841 ttggattcaa ttaatttttt tactccatga atagatttaa tttcattctt aagagaatct
     6901 gttagtggat tattttgttg tatttctgta atatgctcat acaagttatc actcttaatg
     6961 ctaattatat aatcttcgtt atagacaaat tgttgagata gttgtttttt gaaatctatt
     7021 gaattagcaa gattagcgat tgaaataaat aaaactccac taatgcttag cgatattaat
     7081 gcaatcaaat ttttctttct atttgactcg atattcttta atgctagatt ttttatattt
     7141 atataactaa aattttttgc tttttttcta aatttagttt ctgtatattg catggcttca
     7201 atagcagata ttttagaaat aattctagct ggtttaaata aagaaattct aacagttaaa
     7261 aacattacca gtgataaaat taaaattata aataaatcag ctatcatttg ccatgctaat
     7321 ggttgaatta tgtaagcaat tatacaagaa gctataaccc ctaatggaat aaaaataatt
     7381 gacaaaatat ttccttgtag agaaataatc ttttttaact gaaaagaagt catacctaac
     7441 gatttcaact tagcatacat ctgaactgaa ttaacaactg aaatataaaa aatactatat
     7501 ataacaaaaa cgctagaaaa aagaacaact attccaacta ctaaaactgt aataatctga
     7561 tcaccatcaa ttaaatttga ttcaatatag ttatcattta agtatatatt atatttctta
     7621 atacctaaag ataaaccaat tttctttaac tgtgtcttaa ttgaagctga atctttctct
     7681 gtattttctt ctgatagttt aactaataca tctacagtat caggtcttga ctcgtcaaaa
     7741 gtattacttt tattttgtaa aggtgtagaa gctatattct taaaataatc atttgaaacg
     7801 actgtatagt attgcttggc attatcttga taataatttt ctagaaatcc aacaataata
     7861 aaatcacatt gaacttcttt attcgtaata tcatcataat aatttaattt aatgctatct
     7921 ccaatatctt tatctaaacc aaattcctgt aaatattttt ctgaaatagc aatttcattt
     7981 ttatttgtag gtagtttgcc tttcaatggt tgaaaattca taagtttaat tgctgtatca
     8041 tctgcataaa caatcgacat aatacaatct gtctttttat tacttccaat taatttgtaa
     8101 gttcctacgc cactaaagtt tttatcttta tatagtatat tttttgcttt ttcatctaca
     8161 gatttatata tagcatgata tggagatgca ttaactaaat ctacagaggc attatatgaa
     8221 actaaggtca ctgttaataa taaacatgta gaaatcatta taatgatacc tgaaagatat
     8281 gttttaagtt tattaatttt taaatcattt aaagctattt ttcttataaa tgtgcgatta
     8341 ttatttctaa acatatacta cacctcctag ttaacaattt ttccatcttc aatacgaata
     8401 attctatcag ttagttgtgc aatttcatta ttgtgtgtaa tcattacaat tgtttggttg
     8461 aattttttac ttgtcacttt aagtaaactc agtacatctg aactagttct actatcaagg
     8521 ttacctgttg gttcatcagc tagtataata gctggttttg ttacaagtgc tctagctata
     8581 gcaacacgtt gctgttgacc accagaaaga ttacttggta tatcatataa tttttcttca
     8641 agagaaagca tatttataat ttcatttata aaatccttat ctatggtctc tccatccaat
     8701 cctattggta agataatatt ttcatataca ttaagtaaag gaacaagatt ataattttga
     8761 aatataaaac ctatatttct gcgtctaaag attgtaagct gttcatcagt catagtagat
     8821 aattccttac cctttataat aacctttcca gatgtaggat tatctaatcc tcccatcata
     8881 tttaataatg tggatttgcc actaccagat gtacctacaa tagaaataaa ttcgcctttt
     8941 tcaatagata gagttacacc atcaagagct tttgtaatgt ttgaactttg tccataatat
     9001 ttctttaaat taattacttg taaaatactc ataataatac ctccttaaca tcttgtaaat
     9061 acagtataaa accaaaatgt tgcagaatca ttacaaatga aatatttaaa aatttattta
     9121 ttaggtaaga aaatagaaaa agtcgaccct tttccaatat ctgatgtaac tttaatatag
     9181 ccaccttgaa gagatataat ttttcttgaa agatagagac ctattccaat accttctatg
     9241 tcatggacat cttcttcacg ataaaatctt ttaaagattt ctgcttgatg actttctgat
     9301 attccttttc cattatcaat aatatctatt tttgtataca taacccaaca ttctgtagct
     9361 acacgtatct taccattaga atttgtgtat tttacagcat tatctaatat attaaaaata
     9421 gcttcagaag tccatttctt gtcatgaatt atatataaat ttgggtcaca atcaactgta
     9481 acttcaatat tctttttttc agcatcaaac aaaattccac taagtgctgt agcaattgtt
     9541 tcataaattg aatttttctt tttagatagt gtgattatac ctgtctctag tctggaggtt
     9601 tttatcatag attgcattaa aaaatctaat ttatctaatt ggttttccat atccattaaa
     9661 aaatcctttt gaatttctgt tttaaatgtt tgatttagta gagtagtatt aatcattttt
     9721 aaatttgcaa taggagtttt aatttgatga gatatatcag aaactaattc ttgtaaatca
     9781 gctttctcta tagcaatact attgcgattt tcttgaataa cttcatacat tctaattaat
     9841 ctgtgattga tacgtgataa aatagtttct tcttctaatt caatttcaat attgtcttca
     9901 ctattcatca tactatctaa agtgtgacat attttagaac aaaatataac tattttttta
     9961 tgaagtaaaa atattattaa tgctataaat ccacaaagta ccattgtata tattaataat
    10021 agagcaatta gttttaaatc accagttatt agataaattg agatactaaa taaaatagct
    10081 gtaaaaatta taccaacagc aattattaaa taaatagaac taaaagaaaa ttttttaaaa
    10141 ttcatttctt tcacctaacc acatatatcc cataccatat accgttttga tatacttaag
    10201 attgtctttt tcaattttgc ttctaatcct acttataaca gatgttaaag tatgttcatc
    10261 tacaaagtta gcatctatat cccagagttt ttctaaaagt actttccttg ttaaaatact
    10321 tttaggattt tcagtaagca gttttaaagt acgatattct aatggtgtaa atataatttg
    10381 atttccattt atattggcag ataactcaga aaagttaatt tcaagatatc catccctata
    10441 gtaatcttgt tttgttttta ctgtaagatg atttaaaaaa gctttaactt tctgtttaaa
    10501 tatattgata ttaaatggtt ttgttatata atccaaagct ccaagttcat agcctttaat
    10561 catatcacat tccatatcat tagcagttaa aaaaattatt ggtatattgt aattttcctt
    10621 tatttcatta cataattcaa acccactttt atctggtaaa ttaatatcca aaattattaa
    10681 atcaaattca cattcaaaaa tagacttcag tgcagattta gctgtaaatt ttgaagtaat
    10741 tatatatcca tcttcaatta ggttatatga tagagtttta tttaatatag aatcatcttc
    10801 tactactaat attttttcca tacagttact ccttttagtt taatatttta taatgtcaat
    10861 tatagactaa aaacattgct gaattgttat aaacgagaaa aagtatttaa aaatttatat
    10921 atttaataaa gaacctgtcc tatgataaat tctagtacag attctttatt aaataaaatt
    10981 atttcaacca agatggattt ccaggtataa atattctttt accaaatttt gaagtatact
    11041 catctaaagc tttttgaata agctcattat tacccttaaa tgaagtagta ttctttatag
    11101 tttcgagatt ttggctaaat gggcaagcag atatacacat accacaatct gtacctaaat
    11161 atctccattt aatatagcat gtctcatgtt caattaccca attatatttt ccatcatcac
    11221 caattttaat atcattagaa agcgaatgag aagggcagtt aaaagagcat tttttacaga
    11281 ccttacaaaa atcttctagt ccaaaatcaa catattcatc ttctaaaaga ggaatatctg
    11341 tagtaacaac acctaatcta agtctagagc cataatcttt attcgtaagt atagcatttc
    11401 ttcctatatc tcctaaaccc gcatcacgag ctataaaaac aggcattact aaataattag
    11461 catccatatg atttctagca tcatatccta aatttcttat atagtaactt aaaatcatgc
    11521 cgacgataga tgcatccaca tagcactttg atgtttctat aacttcacat atcattggag
    11581 ctctatttat catttcctta tccatctcac atccaaatac aattgcatac ttatgagtta
    11641 gattaatttc ttcaccgtaa ttttcttcat gtctacccct atttgtataa atatgatagt
    11701 cttttaattt agttatacca actacagaag ctccatactg ctttgcaaga cccttaattt
    11761 tttttgtcat tgactttgca tctacatcta ctttagtatc agaaactttt ccttcgcata
    11821 aagatttgat atcagataaa aaatcgaatg cactacttgc cattggagag tttaactcat
    11881 tgtaagtcat tgttccttca ctacataaat ttggtctatt tcttatacta tcatcaattt
    11941 cttttttgtc aggatttttc ttataatagt cattataagc agtactatct tttttgtaat
    12001 ttgctcttgc aaacatagta tttctctcat caaccctttt caattctatt ccccctagta
    12061 aaaatatatt taaaaaatat aactttattg ttgtataatt taattatata agaataaagt
    12121 tatattttca atttaagcat aattaaattg ggaaatatat aactatatgc ttgattatga
    12181 tgtttttatt ttgaaaacaa atttaaatac aatcttttta gtgaaataaa taaaattaaa
    12241 atatttttag tatgataatg caagaaaata attatttatc taaaaaatct aaaggagagt
    12301 aaaattgaac ttattaatta ttgaagatga tataaactta aatgaaggtc ttttttatgc
    12361 atttgagaat gatggattta atgtatttaa ggcttataca aaacaagaag gattaaatat
    12421 atttaatagt aaaaatatag attttataat cttagactgc aatcttccag atggagatgg
    12481 atttgatgta tgtcaaataa taagggaaaa gtcagatata ccaattataa tgcttactgc
    12541 aagagactca gaaattgatg aagtaaaagg attagaaata gggcttgatg attatattac
    12601 aaaaccattt tctttatctg tattaaaggc aagagtcaaa gtagcaataa gaaaaaaatc
    12661 gaataataaa gtaatatatt ccaatggaat aaaattggac caaaagttac taaaagtata
    12721 taaaaataaa gaatgtttgg aacttagctc tgtagaatat aagcttctaa gttatctaat
    12781 tgaaaataaa ggtcaaatat tattaaagga acaaatttta caccatattt gggacagcga
    12841 agaaaattat gttgatgata atattgtatc tgtaaatata agaagactta gagtaaaagt
    12901 agaggatgac ccatctaatc caaaatatat aaaaacagct tatggaatgg gttatttatg
    12961 gaatgaggta gaataatatg tttatattag cctttataat atgtacagtt gttatattta
    13021 tagtatctac taaaataaat cgaaaaagat atgatgaatt aaatttgatg ttaaatcaaa
    13081 tattggaagg taaagaagtt acttatccag atacaaagga tacaagagcg tcaaaaattt
    13141 ctcaccaagt aaaaaaaatt aaggatatga tagaaataga ggtagaacag tcaaagtcag
    13201 aaaaagaagc gattaaaggt ttaatatcaa atatgtcaca tcaattaaaa actccacttt
    13261 caaatataac catttattgt gaacttctgg agaatataaa tatttccaca ttacagaaga
    13321 aggagttttt acaaaagatg aaaaatgaaa cttttaagat tgactggctg ttacaatcct
    13381 tatttaaaat gactaaatta gaggatggag ttatagaatt tgaagtagaa gaactactaa
    13441 ttaaagatac tcttattcaa agtatcagta ctatctttaa taaagcagaa gctaaaaata
    13501 taaggataaa tctcgaacca ttttcagaca taaaattagt tcataataaa aagtggacca
    13561 tagaagcaat tgtaaatgtt ttagaaaatg caataaaata ctcaccatca gatagcacga
    13621 ttactatttc tgtaataaaa atggaactat atacgaagat tactattaaa gatgagggaa
    13681 taggtattga tagtagagaa ctaaatgata tttttaaaag attttacaga agtaaaaatg
    13741 tggaaaacca aaatggcaca gggattggtc tatatcttac aagattaatt ttagaaaaag
    13801 aaaatggaaa cattatagta gaatctaaac taggaagtgg atgttgtttt agtatcttct
    13861 tacagaattg taagagttta aactaatttt gtaggatgag tgtaattatc gtcacttaca
    13921 atattttata gaacatatat ctgtattgaa aggagaaatt aaatgagtat attagtgacg
    13981 aataatttag taaaacacta tggaaaaggt gagactaaag taaaagcctt aaatggagta
    14041 agtatagaaa ttgaacgaga tacatttact gctatagttg gaacttctgg ttctggaaaa
    14101 agcacattgt taaatattat aggtggatta gataatccaa cttcaggtga tgtaataatt
    14161 aaaggaaaga acatatctaa aataggaaaa aaagatttga cagtatttag aagaagaaat
    14221 ataggattta tatttcaaaa ctacagttta atgcccgtat taaacactta tgataatata
    14281 gctcttcctg ttacattaga taaaggaaac catatagacc atgaatatat tgaaatgctg
    14341 atgaagacat tgggaatttg ggataagcgt ttaaaatttc caagcgaact atctggtggt
    14401 caacaacaga gggtagcaat tgctagagct ttggcaaata aacctgcgct tatattagca
    14461 gatgaaccaa caggaaatct cgatagtaaa actacaatgg aagtagtatg tttattaaaa
    14521 gaaagtagtg caaaattcca tcaaacgata cttatggtaa cacacaatga aaatatagca
    14581 caagtatgcg actcaattat ccatattgaa gatggagttg tggtgaatac tggtggtgaa
    14641 gtactatgat gtctctagta aaattttcaa ttcaatatat aaagcattat aaaaaacaat
    14701 ctatatctat agttttaagc atagtactat cagtagcatt attgacggga attggttctt
    14761 tagttcatag tgcagacaaa agtagaattg aaaagataag ggaagatagt ggagattatc
    14821 attatttcta taaagtagat aaggaacaac ttaataaaat taaaaaaaat aaaaaatcaa
    14881 aggaatacac tgtagataga cttggcgtta caaaacctaa aaatcagata gatgaacctt
    14941 ttattataga atttttaaat gctgattctt catatttaga catgacaggt agaaaattat
    15001 taaaaggaac tcttcctagt aaagatggag agatagtact tgacacatat tctctaaaca
    15061 atttaaatgt aaacaaagaa attggaacag aaagcaattt agatggtaaa atctataagt
    15121 tatgtggtat tttatcaagt ctttcaaacc cagaaactgc tctggaggga tttgtaaacg
    15181 attattttca gcctaaagaa aaggattctt atatagttta tgtaaaatat gatgaaaata
    15241 aaaatattaa aaagcaaaat tataaattta tgaaaaaatt caacatatca aaagaaaaaa
    15301 cttatataaa ctggcctctt tcagttgaat ttggagtaaa accaccaaaa aatatagaaa
    15361 aatataatat atttgagttc ttaaaaaatt tagaactaaa tacaaatgca attatattgc
    15421 ttgttggagt gtttggtgca gcagcagtat atagtatttt ccatgtttca atacttcaga
    15481 gaatatcaca atatggggtt ttagaagtat taggcgctaa taataaacaa cttttatttc
    15541 tactattttt agagttattt ttacttttta ttgttggatt tcctataggt tgcttcttag
    15601 gtattggagt agcatctaca atatatgaac aatttccaca tatatttttg agtagtgata
    15661 ttgttccagg agcatttttt ataagtgaaa aatctattta tttaggcttt ttatttatct
    15721 tattgttgtt aattttaatt gctgtacgtg ttgtacatca gttgaataaa tactctagta
    15781 tagaatctat gaaaaacttt aagattacta ataagaaaaa gagaatatta gaaagtgtaa
    15841 aacactctaa catgacaaaa gtattatctc ataaatacat gactcaaaaa aagggaatgt
    15901 ttattggaat tttgttttca ttatcacttg gtggaattat atttttatgt tctagttact
    15961 ctatacagct tactaaaaat aataatgaac ttacaatgaa agctgatgat ggtttaaatt
    16021 ctgactacca aataactatg caaacaacag attttgatgt aggtatacca aaagataaaa
    16081 tttcaagatt aaacaatgtt gagggaatat caaaagtcta tccagtaaga tatttatttg
    16141 gagctatgca aattaaagaa gaacaactat tttggaaaaa tttctttaaa cctcttgaaa
    16201 aagattacag gataaagact ttttttgatg gtatttgtac aaagcaaaat gaaggtggct
    16261 atcttttaaa aacaaatatt tatggttata acaatggtat gttagaagga ttaaattctt
    16321 atattataga tggggagatt aatacccatg atatggttaa aaataataag gttatagtta
    16381 gactacctat ggatggaact ggtatgtatg atgcagttga tataaatcca ggagatacaa
    16441 taaaagttaa agtgccaaag acaatgaaac caacagatga aattgttaaa tttaaagaag
    16501 aaaataagaa tgattataca acaaaagaat ttgtagttgc tgctactgta aaacgtgtga
    16561 tggcaaataa catatatttc attggtgatt atggtatgga tatagttatg acgaatggtc
    16621 aaatgaaaaa taactttgat atcgaaaatt ataattcagt aagtataaaa aagacagaag
    16681 atagtaacag tgaggaattg tctgagaaca taaaaagtgt tgtaagtgat gtaaaaagat
    16741 gtatcgtaac agattatacc attgctattg aaaaaaataa tacctactta aatcaaaaac
    16801 tcttatttat atatggtatt gtatttgtac ttcttgcaat aagtttgttc catataatca
    16861 atacagtaag ctacttaatt ttttcaagaa gacatgagtt tggaatttta agagctatgg
    16921 gtattacgga taataagttt ttacttatga tgataagaga aggtttttta tatggattct
    16981 atgcaagtat tataatggta attggaagtg ttattggaca atttatgatt tattttatgg
    17041 taaaacgtgt atatttatat ataaatccaa ttcttaaaat aaatacacct ttatacatag
    17101 gtatgattat tttaaatatc actattagta tagttgctgt aattattcca gtaagacaaa
    17161 ttttgaagag tgatat
//
LOCUS       DAEMHZ010000085        17172 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000085 DAEMHZ010000000
VERSION     DAEMHZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.085"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(190..558)
                     /locus_tag="KPK72_002526"
     CDS             complement(190..558)
                     /locus_tag="KPK72_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2489730.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIYSFISSLDC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(1086..1508)
                     /locus_tag="KPK72_002527"
     CDS             complement(1086..1508)
                     /locus_tag="KPK72_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG2489731.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1986..4889
                     /locus_tag="KPK72_002528"
     CDS             1986..4889
                     /locus_tag="KPK72_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2489732.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEAEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDMVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4922..5368
                     /locus_tag="KPK72_002529"
     CDS             4922..5368
                     /locus_tag="KPK72_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2489733.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5491..5793
                     /locus_tag="KPK72_002530"
     CDS             5491..5793
                     /locus_tag="KPK72_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2489734.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5974..7380
                     /locus_tag="KPK72_002531"
     CDS             5974..7380
                     /locus_tag="KPK72_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2489735.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7724..8782
                     /locus_tag="KPK72_002532"
     CDS             7724..8782
                     /locus_tag="KPK72_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2489736.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8881..9567
                     /gene="araD"
                     /locus_tag="KPK72_002533"
     CDS             8881..9567
                     /gene="araD"
                     /locus_tag="KPK72_002533"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG2489737.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9585..10232
                     /locus_tag="KPK72_002534"
     CDS             9585..10232
                     /locus_tag="KPK72_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2489738.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(10405..11772)
                     /locus_tag="KPK72_002535"
     CDS             complement(10405..11772)
                     /locus_tag="KPK72_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG2489739.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11962..12050)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12478..14598
                     /locus_tag="KPK72_002536"
     CDS             12478..14598
                     /locus_tag="KPK72_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG2489740.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14702..15955
                     /locus_tag="KPK72_002537"
     CDS             14702..15955
                     /locus_tag="KPK72_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG2489741.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16109..17170)
                     /gene="ytvI"
                     /locus_tag="KPK72_002538"
     CDS             complement(16109..17170)
                     /gene="ytvI"
                     /locus_tag="KPK72_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2489742.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         6764 a         1962 c         2826 g         5620 t
ORIGIN      
        1 atttaatata tttaaattta ttttagatat aaacatcatt ctgactacta ttaaaagaca
       61 tataaaataa ttaaacttaa aagtatattt gcttatcttt ttagtaaata aaatgaaaaa
      121 aagaattagt ggacttatgt aaatattaaa tttaataact tctataaata attccataaa
      181 atacgtctcc tattttcttt tttttagcca ttgttcaagt tcttttatgt catcatctga
      241 tatttcattt ccacaatcta atgatgatat aaagctataa atagaatttt tatgtataag
      301 ctctaaaaaa tcctttgttt caaattttag atattcctca tgtttaactt taatagtgta
      361 taaagtattt ctaccatctt tttcaaattc taaaaaacct ttatcactta atctagaaag
      421 tactttacca gtagtacctt ttgtccatcc atgagaaatt tccatataat tagctacttc
      481 tctggctggt attttatcta tgcctttatc ccatatgaaa ttcataacta acaactcgga
      541 ttttggtatt tttttcaata taatcactca cctttaactt gattatatta gacaaatgtc
      601 taatagtcaa tataatatat cataatgtat aaataatctt tatattattt tatgcttgag
      661 aactaagtag tatagattta tagataaaaa agatattgag agttttatgg aagaggggaa
      721 tatattttct attagagata agaatatgac atatattttt tattgatttg tacaaaaata
      781 ctatatagtg aataaattaa tatcttttct aaattagaag aggataatct tattagataa
      841 agattatcct ctttattgaa agtttaaggg atattagaga ttattaattt ttctctcttt
      901 ttttaactaa aagcaataat acataaaatt attattgcta atgatactct taatgtggta
      961 tatagtcata gtgtcacatg tctatcaata gttttttcag taattattgt aacgaatagt
     1021 aggactcata aaatacttag aaatatgttt ttagataaat tttaaatata taatctaact
     1081 tatatctata aattaacact attattctcc aattccattt ctagtttttt tataaattta
     1141 ttaaattctt ctggttgcct ttttccaaac aatactctta caagagctga attgattttt
     1201 ccaaatatat tcctcttgct ctcactttca ataaaaataa gttttgtatt ttctaagtca
     1261 atatgtacta attcatatct agtaatagta ctttctcttt tattgcttgt ttttatctca
     1321 tatactctat ttttttcgaa attagttatt tcaaccttaa atcttttatt atttttttgc
     1381 tgtacaaatg ttcctactgc ttttttctca ttaaaattag gaaaatttag tcttgcagat
     1441 tttgtaaata tattaaatat gtcttttgca gaatatttta ataaaacttc agttttaaat
     1501 gtattcatat cgttttccct tctgtatata aatttaatat ttataagtta atttttaagt
     1561 taaaatagta taatatgcca tattaaatca tgttaaaact atttatatag ctaaaatatt
     1621 acctatataa gtatgtatac tatcatataa aaataaaatc ttaaaataga aagggttaaa
     1681 aacacagttt attataattt ttattgttga gtagatacgt actattatat atgattgagc
     1741 aagtttttca atcataaatt tataaagtat tttgcattaa aatttaaaca aagagttaaa
     1801 ttatttaaga ttatgaataa ctaatacatt tagtaaatta ctaaatatac aaaatgtaaa
     1861 tacttatcaa aatgtataaa tacttatgac atctagttac ttacagggtt taagtagaaa
     1921 aaaatatcaa agtaagtatg gcatgatagt tgcttatata tttatcaaga aaggagttat
     1981 taagcatgga gttgagagat aaacttgtaa acatcataaa tacagaaaat aaaaaagatc
     2041 cattaacaga tgttcaaata gcaaagtttt taagtacaac tagagagaac ataactaatt
     2101 tgagaaaaga acttaatata agtaattcaa ggcaaagaag atatccttat ttaaaatcag
     2161 caatttcggc aatattacaa aagaataaga atatttctat atcagaaatt acaagggaat
     2221 tgatgacaga aggctttaat atatcgagaa gagttgtgga ggaactttta cctaaagaaa
     2281 gtttagaaat ggatgtagta gaggaagctg aagaagaatc gaatgaccca tttgaaactc
     2341 tgataggtaa taaagggagt cttagaaatg ctgtagagca ggctaaatct gctatattgt
     2401 atccaccaaa agggttacca acgttgatta ttggggaaag tggagttggt aagagtttat
     2461 tttctagaca tatgtatgag tttgcaaagc aaaaaaagat agtaaaagag agctccaatt
     2521 ttgttgtatt taactgtgct gattatagtg acaatccaca attattatta tctttgttat
     2581 ttggatataa aaaaggtgca tttacaggtg ctgaatatga tacaccaggt cttgtagaag
     2641 aagctgatga aggagtttta tttttggatg aaatacatag attgcctcca aaaggtcaag
     2701 aaatactatt ttctatctta gataggggaa agtttaggag attaggtgaa actaatgctg
     2761 aaagaaaagt aagtattatg ttgattgggg caactactga aaatgtagag actaatttat
     2821 tattaacatt cagaagaagg ataccaatgc taataacttt gccaccatta catgatagat
     2881 tactaaaaga gaaaatagac cttatataca atatctttca acaagagtgt aatagaataa
     2941 atgcaaaaat atttgtagat aaaaatgtga ttgaaatact tgcactaaaa aagtttagtg
     3001 gaaatatagg acaattacaa aatatgattc aggttctttg tgcaagagct tttatgaagt
     3061 ttataaattc caaagatgaa gatagtgaat cgatagtagt agtagatata aatgaagttt
     3121 taaagcttaa agattctttt aaagatgctg cctttcaaga gattgaatat acagagataa
     3181 gaaaatattt gaaaaatgct atatttatac catttaattt agaagaatca tcagtaaaag
     3241 gaagtctttt aagtgatgaa tatatattac cagaggacat atacaaaaca attgaaaaga
     3301 aatattatga cctaaaatcc ctagacattg aagatataga tatagagaat attttatgga
     3361 cttttatttt aaatagattt gctgacttaa agtttaatct tgatagtaat gaagaaattt
     3421 tttcaatgag tgatttaaat agttttgtaa gtgatagttt agttaaactg gtaaaagaac
     3481 ttatgaatga tatggttgaa aaaaatatga atttagaagt aaataaaaat atgttcaaat
     3541 atctagcaat acacttagaa gaagctataa aaagaattaa gctaaatcaa aagataataa
     3601 atgtaaattt agaaaaaata aagattgact tttcaaaaga atatgaaatt agtaaaagtt
     3661 ttgcaataaa aattgaggaa tcaattggta ttaagatttc agatgatgaa attggattta
     3721 taacgctcta cataaagtct gctctaaaaa atgaagtgaa aaaagataaa gtagggttaa
     3781 taataatttc tcatggacgt atagctacag agactgtaaa tgtagttaaa gaacttttag
     3841 gtgtaaagtt tcctgtggct atagatatgc ctttagatga aaaaccaata aatatataca
     3901 ataaggcagt agaattgtca aaaataatag accaagggaa aggcatattg tttttggtgg
     3961 atattggttc acttacaaac ataggtcaaa tagtaaataa gagaaccaat ataagtacta
     4021 aaactattga tagagtagac ctcctaatgg cactagaagc aactagaaaa gtttctatgg
     4081 gagaggaaga acttgatgaa atattttttt ctttgctaaa agataggatg ggatataact
     4141 atgaattaga aaaacacata gataaatcaa atgcaattat aacattatgt cttactggag
     4201 aaggaactgc taagtatata agtaaaacgt tggaagaaaa gtatgataac actaaatgtt
     4261 atcaaatgag tgcattggat gaaaatttat tttgtaaaat tgaaaatcta aaagagacaa
     4321 acaatatatt agctattgtt gggacaataa accctaagat accaggtata aattttatac
     4381 cctataataa agaacttttc aaaaatttgg atatctatct atcaaaatca aaacaacaag
     4441 atgacagacc aattaaatcg tatgaaagaa tgcttgatga ggatttagtg atttttgaac
     4501 cagatatata ttttaaaaag gatttattag aatatgtatg ttcaattctt ataaataaag
     4561 aatatgtaga aaaagattac ttagattctg ttttacatag agaagaaatg cttccaacat
     4621 attctaaagg agctataggt gtgcctcatg gagattcttc tactgtaaat agtacaaggt
     4681 ttgtttttgt taaactaaag aatcctattg attggggtgt aggtaatgta aatttcatat
     4741 ttatgccagt ttttcaggct aatgataaag agattgttaa aaatgttctt gagatactaa
     4801 aagatgcaga atttatgaac aatgccaata agtgttttga taaagatgct tttagaagga
     4861 ttatatttga taaatttaaa catctgtagc catagcaaaa aaattgagag gagttagagt
     4921 aatgaatgta gatttaatat cattgggttt agaatctaat gattatgaaa gtgttattga
     4981 agaattagga agtataatgt gtaaaaaaga atatgtaaaa gaaacataca taaatgctgt
     5041 cttggaaaga gaaaggacat taccaacagg tctagatatt ggagaaatgt gtgttgctat
     5101 tccacataca gattcaaagc atgtaaatga gtctaatgta gctgtaggga tattgaaaaa
     5161 tcctgtaaag tttaattcaa tgatagaccc aaaagataga cttgatgtag aacttgtatt
     5221 tttattagca gtaaagaacc cagactcaca agtaaaactg ttaaaagatt taatgtcagt
     5281 ttttcaaaat ataaagttat taaaaaatat aaaaaacgca tcaacaaaag aagaagtagc
     5341 taaattattg gactttatag aaatttaatg tatgagaatt ttataaataa aaaattaaaa
     5401 tttaaacaat caaagtaagt attatttttt taataggttt aatcttatat aaaataaaaa
     5461 tttagagtga aaaattagga ggaaattatt atgaaacaat ttaatgtatt atcagtttgt
     5521 ggttcaggga cagttacatc atctatggta gcaggtaaat taaaagaagt actaggtaat
     5581 aaggggattt ctattactac aacagaagct agaccaacag aagcactaaa tttagcacaa
     5641 tcaggaagat ttgactttat aacatttacg agtccacttc aaacaggcga ttatggaata
     5701 ccaactataa atgcatttgc ttgcctaact ggtatagggg aagaggcatt ctttgaagaa
     5761 gttttgactg ttattgaagg gatagataag taagttttgg agacgtgaat ttatagtatt
     5821 taaatcaatg atttagatac tataataagc taatattaac taaatatttg tggaaaaaac
     5881 attttatatg ttgtcataaa ttaaaaaatt ataaaaatag tcacttaagt attaaattaa
     5941 gcttataaat atttataagt aggagggaaa aagatgttag aatttatgaa atctataatc
     6001 gatacatttg gttctgcaat tatagttcca attataatat tcataatagc aaagatattt
     6061 aaagtaacta ctaagaaatc gtttctttct gctgtgtatg caggagttgc tttacaaggt
     6121 tttacactga ttttaaattc atttacacca ataataacac cagttatcaa taggatggtt
     6181 gagagtacag gagtaaacct accagttttt gatgttggtt ggcaagcaac atcattagtt
     6241 gcattttcaa caagtgcagg tatgatatat ttaggtcttg gtatattatt acaaacaatt
     6301 ttatttttga taaaatggac agacgtgttt caaccatcag atttatggaa taactattct
     6361 tatatggtat ggggagcaat ggttatagga gtaacaggaa attttccttt aggtatagca
     6421 tgtatggttt tattaaacct atatagttta ttaatatcag agttagttgc aaagagatgg
     6481 tctagttact atagatatcc taattgtaca ataatagcta tgcataatgt tgaggcttct
     6541 gtgtttgcag tatttgcaga ccctatatac aataaattgg gattaaataa aataaaatta
     6601 aatccaaaag aattagagaa gaaattaggg tttttaggtg aaccaattac cttaggtctt
     6661 tttttaggta tgtttatagg tatattaggt aatatgacga gaataaatac tatggaagct
     6721 tggggagaaa taatgaaagt aggtatatct acaagtgcag tgatggcaat tttccctaaa
     6781 gtagcaagta tgtttgctca agcatttgca cctataacag aagcagctag aaagattatg
     6841 caaaaagctg gaaaccgtga gtggtacata gcagtaaatg atgctgttgg atatggtgaa
     6901 cctgcaactt taatatcagg tcttatatta attccaataa tgcttgtaat agctatggta
     6961 cttcctggta ataaggtatt accagttgtg gatttattgg caattccata tatggttcaa
     7021 ggtttagttg ccatacataa tggtaatata ccaaaagtat tagtttcagg tataatttgg
     7081 tttggattag gattatatgt ttgtacatct acagcaccat tatttacaga tatggctaca
     7141 aatataggtg tagctatacc tgcaggagca atgttaataa caagctttaa tatactagga
     7201 aaacctttga tgggattagt tttctttgca tttctaagtg caaatccaat ttatataggt
     7261 ctttcagttg taatttactt tgttctatgg gcattgttta gaaagaacaa aacaagtatt
     7321 cttgattatt tagaaaaaca agcacttaaa aatgtagaag aagagcctgt agcagtttaa
     7381 gtttataaaa atgaaataaa aataattaaa ggagtttata attgatttta tataagtatc
     7441 agataatacc tgagaaatat ttaaatgaaa tatagacttc tttttatttg ataatatttg
     7501 taattataat gcattgtatt tttatagttg agttttctgt ataacctgct atttaaaaca
     7561 tactctttta tatttataca gtaatgtggt ttaattttat aaaatgtaat ctctactggt
     7621 aaaagacttt gtatataagg catttgcctg aataaaccat ttatcttttg tatggcatag
     7681 atattgctat atagaagtat atagcatgaa aggagatatt tatatgggaa ataaaatgag
     7741 agcatcagta ttgtataatg taggtgatgt cagatatgaa atggtagata taccagaaat
     7801 aacagatacg caagtgcttg taaatgtaaa atatgttggg atatgtggtt cagatttacc
     7861 taggtctatg gtaagtggtt tgagtggaaa tacaaaatat ccattgattt tagggcatga
     7921 attttctggt gaagttgtta aaattggaga aaaagtaaag catattaatg taggagatag
     7981 agtagctgtt gcaccattag taccttgtgg aaaatgtgat tattgtaatg aaggtaattt
     8041 tggcttgtgt gatgattaca atataattgg aacaagagta aatggagcgt ttgcagaata
     8101 tgtaagagtt ccagaagaac atattttaaa actaccagat acattggatt atgaaactgc
     8161 tgccggaatt gaaccagcaa ctatagctta tcatgggatt tcaaagtcta atattagagt
     8221 aggagatagt gtagttgtgt taggatgtgg tccaataggt cagtttgtta tacaatgggc
     8281 aaaggtgttt ggtgcatcta agattatagc agttgatatt tttgatgaaa aattagaact
     8341 ttctaaactt ctaggagcaa actatatact taactcaaaa gaagtaaatg taatcaaaga
     8401 aataaaaaaa ataacaaatg gtggtgctga tgttgtaata gaaactgctg gtagccgatt
     8461 tacacaagaa caatctctat ttgtagcaaa aaagagagga aatatagtgt ttgtaggtat
     8521 atcacacaca gaattaccac ttagtgcaga cgctactgag tgtatattac gtggagaact
     8581 taccttgaaa ggttcttgga attcatatac aagcccttat cctggaagag catggactgc
     8641 aacattggat tttatgggaa aaggtgacat tatttttaaa cccatgattt cagataaaat
     8701 aggtcttaat gaagtgggtg actttttaag taaaatgagc aagagagaaa ttaatttcaa
     8761 taaaatccta gtagaaatat aaaaatttaa taaaaaattc taaaagcaat taaattttat
     8821 aactatgaca ttaaataaaa taaaaataat tttcttaaat aaaatatagg aggaagaaaa
     8881 gtgttagaaa atttaaaaga agaagtattg aaagcaaact tagaattacc taagaaaaag
     8941 ctaattactt atacttgggg aaatgtaagt gggatagata gagaaaaagg attagtggtg
     9001 ataaaaccaa gtggtgtaga gtatagtaat atgactgtag aagatatggt tgtagttgat
     9061 ttagagggaa atatagttga tggcaaatta agaccttctt cagatactcc gactcatcta
     9121 gttttatata aagagtttga agaattgggg ggaatagtac atacacattc aagttgggca
     9181 acaatttggg ctcaaatagg aaaatctata ccatcttgtg gtactactca tgcagattat
     9241 ttttatggaa gtataccatg taccagaaaa atgactagag aagaaatttg caatgaatac
     9301 gaaaaggaaa ctggaaatgt aatcattgaa gaatttatgg gaagaaatcc aattcattgt
     9361 ccaggaataa ttgtaaatga ccacggacct tttacatggg gaaaagatgc aaatgaggct
     9421 gtacacaatg cagtagtttt agaagaagtc gcaaagatgg cttattatac agagttaatg
     9481 agtcctgata atattatgga taaggttctt atgaataagc atttttcaag aaaacatgga
     9541 aaaaatgcat actatggtca aaaatgatta tctggaggtg aattatgcta gactctttaa
     9601 aaggtaaaat tgtggaaata gcaattaaag cagagcatta tggtttatgt aaagaaaaga
     9661 ctggtaattt tagtattaga gataaggata ctggatatat acttataacc ccatcaggta
     9721 taggaagaga agaattaaaa gttgaacata tttgtgtatt ggatttggat ggaaatgtaa
     9781 tagaagttga gaaaggtatt aagccaagta gtgaggtatt gatgcatatt gaaatataca
     9841 aaacaagaga aaatataaat gccatatcac atactcattc tttatatgca acagcatttt
     9901 cagtagcaaa gaaaccaatt ataccaattg tatatgaatc agttaattat ggcggttatg
     9961 tagatattgc tccatacgaa agaccaggaa ctttaaaatt agctaagagt gtttcaaaat
    10021 tactgataga tacagatgca tgcttattag aaagacatgg gttgacaact gtaggtaatg
    10081 atttagacga agcattatta aaatcaagat atgtggaaga ggtagcagaa atatactata
    10141 ggtctttagt attaaatcaa tttaaagaac ttgaagtagt agatgttgat gagtttaaat
    10201 cttggaaata cccagaagaa ataaagtttt aatctaaata attttcattg aagtggttaa
    10261 ttaaaatata tgtattattt atatcaatta ctatgtttta ttatgttaat taccgtattt
    10321 tgcaaatata tttattacat tttttcaaaa aaatatagaa tccgaaagcc atataaaatt
    10381 gacttgggat tctatacttt ttatttatgc atttttcttt atctgatttt ctggagaatt
    10441 tatatgttca aaatattttt tagtttcaga tactattata ggtgataatg caagtaaagc
    10501 tataaggtta ggtattgcca taagtccatt tacaatatca gcaattatcc aaacaacttc
    10561 taactttaag aaagcaccta ataacaccat agccacaaag acagttctat acccattcat
    10621 gccttttaca ccaaacagaa attcaaaaca tctttctcca taataactcc atcccaaaat
    10681 tgtagtaaat gcaaataatg ttaaactaac tgtaagtaat attggtccaa atgtaggaat
    10741 tgctccgtta aaagctgctt gtgtcataac agctccattt aggtctgaac accacactcc
    10801 tgaaataaca agagagaatc cagtaagagt acagattata atagtatcta taaaagtacc
    10861 tgtcatagat ataagacctt gttccgctgg ccattcagtt ttagcagcag ctgcagctat
    10921 tggagcacta cctagaccag actcatttga aaatacacca cgagcgatac catttctaat
    10981 tgccagtgac atagttgctc ctaaaaaacc accagtagca gcagtaggtg taaatgcact
    11041 tttaattatt aacataaaag tttcagggat tttattccaa aaagatatta aaatgattcc
    11101 acagattaca acatatataa ctgccataaa aggtactatt ttagtagcta cattagatat
    11161 acttttaagt cctccaaata caaccaaagc tacaaatact gctaatacaa cccctgtaat
    11221 tctagggtct atcccgaaac tattatttat tgcatctgtt attgaattta cttgagtaaa
    11281 agtaccaatt cctaaaagtg caactagtat accacttata gcgaaaaata ttgcaagagg
    11341 tctccatttt tctcccatac catttactat gtaatacatt ggcccaccag acacttggcc
    11401 atttttatct ttagttctgt attttattgc aagaacacct tctgaatact tagtagccat
    11461 tccaaagaat gctgctatcc acatccaaaa aagtgcacca ggtccaccag ctttgatagc
    11521 tgtagcaaca ccaacaatat taccagtacc aactgtagca gctagagcag tacataaggc
    11581 tgcaaaactt gaaacatcac cactaccttt gttttcagca gagaatatta acttaagagc
    11641 tcttggcagt ttagttattt gaacaactcc aagcttaaca gtaagtagta taccagtacc
    11701 aactaatagt accagtaggg gtggtcccca aataaaagca tcaactttgt ttaaaatatc
    11761 aattagattc ataaaaacac tccctcattt tttttgcttt acataatcaa tagaattttt
    11821 aatgaagtaa ttgacaggaa ataagtataa tttattaata atttctagtg taataaaaaa
    11881 agctaaatct aaaaaagatt cagctctgga tgtacactaa ttagcaacca aaataaaagt
    11941 attagtaaat actgtttttg gtagataatc cctgtccttt tgcctgagag attgagcata
    12001 aaagccttgc cccttcggcg cccataatgg gtctctccag agttccgtaa attttacagt
    12061 cctactttta aaagtacctg agagtgttac tccttcggtg tgctatatag caactcttct
    12121 ataaaatctt aaccggatat tgatttgtca tagcttattg tattaataat attaatataa
    12181 gtttttatgt tttgtcaact aaaaaattaa aaaacaaaga aaaaatataa aaatgtaagc
    12241 ctatttacga attataaact tagaaagatg agtaatatac gtgaaaatga attttgtaat
    12301 acacatacaa atgaagtgta aaataagttt taaataaaaa aataataaaa tcttaaataa
    12361 ttaattagat atgattaaat tatggtaata ttatagttac gatattaaga aaagttctat
    12421 ttagttagat tttaagcgta atatgagatt atgtatacag aaaggatgta tttttagatg
    12481 ggaaaaatat tagtgctagc agaaaaacct tctgttggaa gagatatagc aaaagtttta
    12541 gggtcaaaaa atgaaaaaaa tggttatata gaggggccaa agtatgtgat aacttgggca
    12601 ctaggtcatt tggttacact tgctgacccc gaaagttatg gagaaagata taaatcatgg
    12661 agcttagaag acctgccaat acttcccaag caccttaaga ctgttgtaat aaaaaagagt
    12721 ggaaagcagt ttaatacagt taaatcgcaa atgaatagaa atgatataga tgaaatagtt
    12781 atagcaacag atgcaggtcg tgaaggagaa cttgtagcaa ggtggataat agaaaaatca
    12841 caggtaaaaa aacctataaa acgtctttgg atttcttcaa gtacagacaa agcaataaaa
    12901 gacggatttg caaaattaag aagtggaaaa gaatatgaaa atttgtatta ctcagcaata
    12961 gcacgagcag aagctgattg gattattgga ataaatgcaa ccagagcttt gacaacaaag
    13021 tacaatgcac agctttcttg tggaagagta caaactccta ctcttgcaat gttgcttaaa
    13081 agagaagaag aaataagaaa tttcaaacct aaagagtatt atggattaga attaattgct
    13141 acaaagggaa attcagatat aaaatttatt tggaatgata aaaacaataa ttcttcaact
    13201 tttagtaaag aaaagataga atccacatta aaaaaagtaa aaggtgtaga cataaaaata
    13261 tctgaagtaa acaagtcata taagaaaaaa tattcacctg cactctatga tttaacagaa
    13321 ttacaaagag atgcaaataa aatgtttgga ttttcagcaa aagaaacttt atctataatg
    13381 caaaaactgt atgaacatca taaagtactt acttatccta gaactgattc tagatattta
    13441 acatcagata ttgttggaac attaaaagat agaataaaag ctgtaaatat tagtgattat
    13501 tcaaagactt gtaataaatt attaaaaagc aatattatag ctaataaatc gtttgttgac
    13561 aattcaaagg taacagacca tcatgcaata ataccaacag aacagaaggt attcatgagt
    13621 gattttagtg acaaagagcg aaaaatatat aatcttgtag taaaaagatt tttagcagta
    13681 ctatctcctc cttttgaata tgagcagaca actataagtg caactgtaga aggagaaata
    13741 tttaaggcta agggaaacaa agtaataaat ttaggttgga aagaaagtta taaagatatt
    13801 gaagatgaag atgagtatga aagtatacca aatattttag aaaaagatgt acttaaggtc
    13861 aagggattaa agataacttc aggaaaaaca aatccaccac catacttaaa tgagggaaca
    13921 ttattgactg ctatggaaaa gaatggtcta gggacagttg ctacaagagc agatataata
    13981 gaaaaattat tcaattcatt tttaatggaa aagaaaaata aagatatata tataacatca
    14041 aaaggaagac aattattaga tttagttcca gtagacttaa agacaccaga acttacagct
    14101 tcatgggaaa agaaattatt ggatatttca aagggaaaac ttagtaaaaa tacattcata
    14161 aatgacatga aagattattc aaaagatatt gtactagaag taaaaaatag tgataataaa
    14221 tttaaacatg ataacttaac taaaaataga tgtccagaat gtggtaaatt tatgttagag
    14281 gtaaatggta aaaaaggtaa gatgttgatt tgtgaagata gagagtgtaa tactagaaaa
    14341 actatatcac aagtaaccaa ttcaagatgt cctaactgcc ataaaaaatt agaacttcgt
    14401 ggagaaggtg aggggagact cttcacttgt agttgcggat acagagaaaa gttgtcttct
    14461 ttcaacaaga gaaagcaaga agaaaaacag aaggcatcaa aaaaagatgt taatcaatat
    14521 ttaaaaaatc aaaataaaaa tcaagagaca tttaataatc catttgcaga agcattagct
    14581 aagctaaaaa acaaataatt ttaaagttat tattatacac aattataact ttaactgtat
    14641 attagttata tgtactgata taatatagtg tatagtttag gcatgaaagt agggctgtta
    14701 aatggattta agtggcatat ttaaatacta ttgtaaagag tgtgaaaata catggaataa
    14761 ttcgagtgtt gaattatttg agaatataga aacgtatagt aaagattcac aaaaaaagag
    14821 ggaaaaagaa ttagataaat tgctaaatac aatatcagtt catttagaga ggtatccaag
    14881 tgatgctgta ttgagaaaaa tgtgggtaaa aaagggcgag gttttcttac aaaagacatt
    14941 ggaaaaagaa aatattttta agttagaaaa aatggatgta gaggatagaa aaaaattttt
    15001 agatataaca aaacagttta ttagagatgc tagaaaattt gatgatgatt tacctatagg
    15061 tgatattatg caagctatga ggaatgtatg gatttcaaat gcattacaat tattatttgg
    15121 taaagaagta tattattcaa aagctaactt tgcatatagt atgttatatc catatactga
    15181 taattattta gacaatacaa atatagataa aaatgataag attttattta ataactggtt
    15241 agaaaaaagg ctcctgggag aacacattaa atctaaggat tatcatgaaa gtaaagtatc
    15301 tcaaatgata gattatattg aaagtgtata ccctagagaa aagtttacag aagtttatga
    15361 atcgttatta ttaatattta aaagtcaagt aaatagttta aaacaacatg gtaaggaaaa
    15421 tcatttgtgt aaagaagatt tattatccat ttctattgaa aaaggaggtt catccgtttt
    15481 agtagatgga tatttaataa gtggattgat gacaaaggaa gaaatagagt tttgtatagg
    15541 atatggattt ttattacaaa tatctgatga tttacaagat ataaaagaag atttaaaata
    15601 caaccataaa actattatta cagagatgtc aaaagagggt actttagata aagttgtaaa
    15661 taaactaata aattttacta ttgagttaat agatagtttt aaaattaata ataaaaataa
    15721 atctgtaata actatgataa agaatgattg cttaatgtta attttatttt ctgtagttta
    15781 taatgctgaa tttttttctg taggatatat aaaagaagta gagaaattta ttccatatac
    15841 aatagattat tcattagaga ttgaagaaaa aataaaagaa aaatttaaaa atatagatgt
    15901 tttaaataat gaaaatgaat ataaagaaat gattgatatt atttgtgcag agtagaaaaa
    15961 aagggagtgt cttagaatag actttttagt ttatgaggca ctctttttgt ataaaatcaa
    16021 atgtattttc atcattttaa caattaaaaa gagcttatcc ctatttgaga aagccctttt
    16081 taatttaaat aaaaatgtca aggttaaact atttaaatgc agtaataatc ccattatcat
    16141 gtaagttttt tatgattgta gctgcgatag ggaatccaaa taatccagct attccaaata
    16201 gttgagcccc aacaaacata cacattaatg ttattaaagg atataggcca atttggtgtc
    16261 ctactacctt tggttctaat atttgtctaa caacggttat aattatgtat agtattaaaa
    16321 gtcctattgc taaattaata tttccattaa tgagtgagat aatcatccaa ggtgtcaaaa
    16381 tacttcctgt acccaagact ggcaaaatat caacaagtgc tatgatgaca gatatagtaa
    16441 atgcattttc aacctttagg atagtaagtc caataagtag ttctatgaat gtcactgata
    16501 gtagtattgc ataggctttt agtagtttta aaagagcatt aatactgcta ttttttatat
    16561 ttattatcat aaattgactt ttttctggaa attgttttaa gataaagttt actatttttt
    16621 gatagtcaaa agtaaagaaa aaagaagcaa ttaaagtaat aataagtttt aacataaatg
    16681 caggtgtttt acttgcaata ctagcaatag cattgagtgc atttgttgaa atagaagcta
    16741 ctaaagacat catagattga cttatattat cccaaccaag tattaatgct aggttaacat
    16801 taggtgtact agcatctatc tttgaaaata aagtatttag cgttggttgt atacttgatt
    16861 tatatatttc aggaagtcta aaaaatacat cacttacact tgcaaatatc ttcacaccaa
    16921 agctaactaa taaaaatata cacaacccat aaaaaattaa aagtattact atagaaataa
    16981 gtgtacgatt taaatttgta ttatttgtaa taagtcttat aataggtctt aagatggatg
    17041 caatgataaa actgattaca aaaggcatta ataatggcaa taagaatttt aatacaatat
    17101 atacaatact tgttattaaa actacatatg taaatttaat tataaaattc ttttgctttt
    17161 gaatatccat aa
//
LOCUS       DAEMHZ010000086        16990 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000086 DAEMHZ010000000
VERSION     DAEMHZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.086"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..793
                     /locus_tag="KPK72_002539"
     CDS             59..793
                     /locus_tag="KPK72_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489743.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSKGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1004..2185
                     /locus_tag="KPK72_002540"
     CDS             1004..2185
                     /locus_tag="KPK72_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG2489744.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2169..2741
                     /gene="mobB"
                     /locus_tag="KPK72_002541"
     CDS             2169..2741
                     /gene="mobB"
                     /locus_tag="KPK72_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG2489745.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            2860..3135
                     /locus_tag="KPK72_002542"
     CDS             2860..3135
                     /locus_tag="KPK72_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2489746.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3402..5102
                     /locus_tag="KPK72_002543"
     CDS             3402..5102
                     /locus_tag="KPK72_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489747.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5465..6652
                     /locus_tag="KPK72_002544"
     CDS             5465..6652
                     /locus_tag="KPK72_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489748.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6712..7875
                     /locus_tag="KPK72_002545"
     CDS             6712..7875
                     /locus_tag="KPK72_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2489749.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADLFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8026..8829
                     /gene="zupT"
                     /locus_tag="KPK72_002546"
     CDS             8026..8829
                     /gene="zupT"
                     /locus_tag="KPK72_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG2489750.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGIAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8925..9485
                     /locus_tag="KPK72_002547"
     CDS             8925..9485
                     /locus_tag="KPK72_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG2489751.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILLMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            9735..10448
                     /gene="rgbR"
                     /locus_tag="KPK72_002548"
     CDS             9735..10448
                     /gene="rgbR"
                     /locus_tag="KPK72_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG2489752.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            10442..11821
                     /locus_tag="KPK72_002549"
     CDS             10442..11821
                     /locus_tag="KPK72_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2489753.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGVLIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLFSQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            12062..12739
                     /locus_tag="KPK72_002550"
     CDS             12062..12739
                     /locus_tag="KPK72_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489754.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIKFIYN
                     LFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKLSLVY
                     KFDISNH"
     gene            12960..15332
                     /locus_tag="KPK72_002551"
     CDS             12960..15332
                     /locus_tag="KPK72_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG2489755.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            15347..16249
                     /locus_tag="KPK72_002552"
     CDS             15347..16249
                     /locus_tag="KPK72_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2489756.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            16269..16820
                     /locus_tag="KPK72_002553"
     CDS             16269..16820
                     /locus_tag="KPK72_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489757.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
BASE COUNT         6742 a         1648 c         3065 g         5535 t
ORIGIN      
        1 taactgtaaa ttgagatata gataaatact ataaaattta agaaaggagg attgatttat
       61 gaagttaaaa aggtcattag tttgtgtaac tattttaggt attatacttg ttggatgtca
      121 taaagaaaat actaaagaga aaaatcaagt agcaagtaaa gcaacacagc aaaaaactat
      181 gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa gattataagt acattataaa
      241 gaatatgggt ataccttata atacatttta ttatataaaa cctaaagttt taaaagaatc
      301 aaatacaatg caagatataa atacatcaag ttatatgaca ttagtttatc caaaatatac
      361 tggaaatgat gaattagatg gaagtgcttt atatgttgat attaacgaaa ataaagttgt
      421 caatgttgaa accaattctt tctcatcaaa aggtatatca gtcattgatg ctgaatctag
      481 tatagtgatt gaaaaaagtg accatgaaaa atcggctgta tcactagaaa attttagaca
      541 tattgacctg ggagagtatg ttggtgttga agattccaga ataaatgaaa ttgtaggaga
      601 tgcgaattat gatttaacag catataatca tgaaggcagt aaagtagtta agagttatag
      661 attgaaagaa gataacaaaa tattaaaaaa agaggtactt acaataagta ttgtggataa
      721 caaaataaaa tctataaaaa ctattgaaag tgataaaatt gtaaaaataa taaaaggaac
      781 tttattagaa taaaatattc taaagcatat gatgctgttt caagatagaa ggaaatttct
      841 agctgggaac agttttttta ttggatttat aaaatttgaa ctgcacttat agataactat
      901 tagttataat taaatataag ataaaagtat ttccaacttt tataatctat aatatattac
      961 atagaaatat cttaaataaa aaatatattg gggagtattt aaaatgatag aattagaaga
     1021 tgccataaaa attattgaaa aaaatgtttt accaataaac agggtcaaga tagtaaatct
     1081 tatagatgca gtaaataagg tagttgcaga agatatatat tcaacaattg atagtcctcc
     1141 atttgatagg tcaccctatg atggttatgc atataatgca aattctgaaa atagaaattt
     1201 aaaggttata gggactttgt atgcaggaga tgtatttaat agggtattaa ataaaaatga
     1261 agcagtgaag ataatgactg gtggaaagat accacagggt gcaaattgtg taataaaaaa
     1321 agaagatgta acagttatag gtgatatgat aactttaaat ataaaattaa aggaatacga
     1381 taattacata tttaagggtg aggatataaa aaaggggcaa ttgattatta ataaaaatca
     1441 gaatatagga tatgatggta ttggagtatt agcaagtctt ggaatatcca aagtaactgt
     1501 ttatgatgat ttaaaagttg gaattttgaa tacaggtaca gaactacaag atataaatga
     1561 gactttagag aatggtaaaa tatacgatag taatagatat accttatatt caagattatt
     1621 aaaacttgga atacaagcag atacaatatg caattgttct gatgatatat tggagttaga
     1681 aaaaaaggta aagaatatgt tacagagtgt agatttttta attaccacag gtggtgtttc
     1741 tgttggagat aaagatttaa ttccagatgt attcaaaaaa ataggggcaa cagaattatt
     1801 ttggaagata aacattaaac ctggtggagc ttgttatgta ggtacattgg gagaaaagct
     1861 attatttggt ctttctggta atccacatgc agcaatagtg gtatttgata atgtgatagt
     1921 tcctgttatt gagtatttaa catataagaa gggaacacat catataaaag ctatatttaa
     1981 aggagaattt aataaacttt caaataaaga tagatttgta agtggaaaac tatatacaag
     2041 tgaaggtaaa ttgtatgtag agctaaatga taagaaagat gcaaaagcta gattatctgc
     2101 aactctaaga agtaattgta tgattaagat taaaaagaat gagacaataa aagaaaatca
     2161 attagtggat gttgttatta gatagtttta tgaaaggaca caattttatg tataatatat
     2221 tgtcagttgt ctcatattca ggagtaggta aaactacatt gattgaaggc ataataaaag
     2281 aattaaataa aaaagggtat cgtgttggag ttattaaaca tacctgtcat gattttgata
     2341 tggatgagga aggaaaagat acttataaac atagaaaatc tggggcgaga aaagtctgta
     2401 taatatcaga taaacgagta gcatatattg aagaactaaa agagaagaat ccattatata
     2461 aaatgattca attatatgaa gatatggact tgattatcat agaaggatat aaaaattata
     2521 gatttaaaag attggaagta actagaaaag gtaagtatga ggatatttta tctaataagt
     2581 ctgatttgat aggtattgtc agtgatatag aatataaatt gaatattgaa cagtttaatt
     2641 taaatgatta taaaaatata tcaaagtaca tagagtcttg tataaaaaat gaaattttaa
     2701 gtataagcaa tgttgaaata aaaaacatat taaaagaata attttaagat gttcttctta
     2761 tataaaatga tataattatt tatataaata aagatataag aaatttatca attttatttt
     2821 gagttataaa atattaaaga aattgtagga ggacaatata tgaaaaaaat attagtagcc
     2881 tgtggagcag gaatcgcaac atcaacaata gtttgtgaca gagtagaaag attggttaaa
     2941 gaaaataata tacaagcaga agttgagcaa tgtaaaattg ctgaatgttc aacaaaacaa
     3001 gaaggtgctg atttaatcgt atctactact atactaccaa caacttatga tatacctaca
     3061 ataaaggcaa caggatatat aacaggtata aatacaacag cattagataa aaagatatta
     3121 aatgcattga agtagttttg tatttataaa aaaaatttag tattaaatat agagttgtat
     3181 ctttataaaa ttttaaatat agagatatag ctctttttag gttgtttcaa gtatacaact
     3241 atatgatata aatcttcaat tttatgaaaa agattgttat tactttgtaa gtttataaaa
     3301 gatttaaagt tattgttatg ttatactaat ttatgggtat tttttagaag tactaataga
     3361 agtactaata gaagtactaa taaaggaggg atttaatact tatgataaaa caatttgtca
     3421 aatattataa gccatataag aaaattttta cactagacct tatagcagct tttctatttt
     3481 ccttatgtga tttagtttat cctatgataa ctagaaatat aatggatgac gttgttccaa
     3541 ataaaaactt aagaatgtta gtagtttttg cagtagcact tatacttata tttatagcaa
     3601 aagcagggct taactatttt atgcagtatt ggggacatgt tattggtgta gatatgcaag
     3661 ctgatatgag aaatgaggta tttacacact tacaaagact tccaaatacg tattttgata
     3721 ataacaaatc tggagtaact atgtctagaa tagtaaatga tttaatggat ataacagaac
     3781 ttgcacatca tgggcctgaa gatttattca tttctatagt aatgttggtt ggttcattct
     3841 ttatacttat agatataaat attcctctta cgcttataat atttgctata ttaccgttta
     3901 taatttggtt tgcaatagct aaaaaagata agatgaatat tgcttttatg aagagtagag
     3961 ttactatagg tgatgtaaat gctactttag aaaatagtat agcaggtatg aaggtaacta
     4021 agtctttttg tactgagaaa gaagagttaa ataagtttgt aagaagcaat aaattattta
     4081 gaagagcaag acaagattct tataaggtaa tggcagaata ttactctggt atgaatttat
     4141 acatggatat cttagagtgg gtagttgtaa tagctggtgg ttattttact tatataggaa
     4201 agattacttt gggtgatttt gctgcttata tactatatgt aaaaatgttt atacaaccta
     4261 tgaaaaaatt aataaacttt acagagcaat atcaaaatgg tatgacaggt tttaaaagat
     4321 tcatagagat aatggaacaa gaccatcaaa aagaagctaa aaatcctatt gaacttgaaa
     4381 atgtaaaagg tgacattgag atagaaaata tatctttcac atatgaagat aagactcaag
     4441 ttttggataa tttaagttta tctataaaag ctggtaagac tatagcatta gttggacctt
     4501 ctggaggagg aaagactaca ttatgtaatc tattgccaag attttatgaa tttgataagg
     4561 gtgatataaa aattgatgga aagagtataa aagatgttag tttaaaatca ttgagaaaaa
     4621 atataggtat agttcaacaa gatgtatttt tatttacagg aactataaga gataatattc
     4681 tttgtgggaa ccctaatgct acagatgaag aaatgatagc tgctgctaaa aaagctagaa
     4741 tacatgactt tgtagagact ttgccagatg gatatgatac ttatatagga gaaagaggcg
     4801 taaaattatc aggtgggcaa aaacaaagaa tttcaatttc aagaatcttt ttaaagaatc
     4861 ctccaataat aatattggat gaggctacat ctgctttaga caatgtaaca gaaagagaaa
     4921 ttcaagagtc tttagaggaa ttaagtaaag atagaacaaa tttagttgtt gcacatagat
     4981 tgactactat aaaaaatgct gatgaaatca tagtattgac ggataaagga attgaagaaa
     5041 gaggtactca tgaagagcta gtaaacaaaa atggagtata tagtaggtta cacaataact
     5101 agtatacaaa ttttacacca tagctaacat acacattaaa cttacaaaca taggatatat
     5161 taagattcat aaagacaatt cattttatat ttataagatg ttttatgaat aaaatgtatc
     5221 ctatgtttta ttttttgcaa attacattta attctctttg taagaataga gttaaatagc
     5281 caatggtcgg gataacgttt ttacaaaaag aataatttag aaaactaact ttaatttatg
     5341 gaatttatcg aattttatga aaaaaatcga attttgactt gaaatgtttt tcaaaagtag
     5401 taaaataatg gataactata catacatttt ataaaaatat atttattatg aggggggatt
     5461 ttgtatggag gcaataacag ctttttgtct agttttagta tcactgacga taggagatat
     5521 agtttcagca aaaactaaag cttttgttcc gtcggttttt gtatcagcaa ttatatttat
     5581 agtaggattt tggacttttt tcccagaaaa tattgtggac ttagccgctt taggaacacc
     5641 attagctcaa ttaggtatgc ttttgttaat aactcatatg ggaactatga tgagtattaa
     5701 ggaattagca ggtcaatgga aaacaatagt aatagccttg gcaggaatag taggaatatg
     5761 tgtaggtgct ttggcattag ggacagtagt atttggatgg gatacagcag taatagctac
     5821 acctcctctt actggagggc tagttgcatc tataatgatg gcagatgctg ctacagcaaa
     5881 aggtcttgca agtttatcag tacttgcaat tttgatgtat gttgcacaag gatttgcggg
     5941 ttatcctatt acagcattaa tgcttaaaaa agaaggaaaa agacttttat cagactttag
     6001 aagtggaaaa gtaactgtaa atacagatga agaaaaagtt aaggatttac ctgaacaaaa
     6061 aagcagattt aagataatac cagacttacc agaaaaatat gatacgacat atatgataat
     6121 acttagatta ggatttgtag catggttagc agtacaattt acaaatctta caaatgaagt
     6181 tgtatcaaga tatgttatat gtttaatatt tggtgtaata gcatcagaac ttggaatagt
     6241 tgatagaaaa ccacttaata aatctggttc atttggatgg ttaatgacat cattgatggc
     6301 ttttatattt gcagggttag caaaagctac accagcaatg cttgttgaaa tggcagttcc
     6361 tcttgtagga atcataatat ttggtgtaat aggtatggga atattctcta ttatagtagg
     6421 taagctttta ggtgaaagta aagaaatggc tttttcagtt tcgcttacat cactttatgg
     6481 ttttccacca aactatatat taactgaaga ggcagtaaag gctttggctg aaacaccaga
     6541 agaaaaagaa tttttaatgg ataagatgct tccaaagatg cttgttggag gatttactac
     6601 agttacaata gcttctgtag taatagcggg tatctttgta aatctaatat agtatttgag
     6661 tgataaaaag tattttaaat aagaagaatg tgtactactt ggaggtatgg aatggatatt
     6721 aaagaaataa caaagagtta taaagactat gtaattaagc ttagaaggga atttcatgaa
     6781 aatcctgaga aaagtatgga agaagttaga acatcaaaga gagttaaaga agaacttgat
     6841 aaaataggga taccatatgt atcagctgga ggtacaggtg taattgcaac tataaaagga
     6901 gctaatccag gaaagactgt agcacttagg ggtgatatgg atgctctaca agttgttgaa
     6961 tgcactgatg tagaatacaa atctaaaaat gaaggactta tgcatgcttg tggtcatgat
     7021 ggtcacacat caatgcttct aggggcagca aaagtgttaa atgatattaa ggatagtatc
     7081 aatggaactg taaaattatt ttttcagcca ggagaagaag ttggtaaagg tgcaagagct
     7141 atgattcaag atggtgctat ggaaggtgta gatagtgtgt ttggaataca tctttggact
     7201 gatgtagaat ctgggaccat atcggtagaa gaaggaccaa gaatggcttc tgctgattta
     7261 tttaaaatta ctgtaaaagg tagaggtggt catggttcac ttcctcatca aggtgttgat
     7321 gcagttcttg caagttcagc aatagttatg aatctacaat ctatggttag tagagaagta
     7381 agtcctctgg aaccattagt tgtgagtgtt ggagttttaa attctggaac tagatttaac
     7441 gtaattgcga gtgaagctgt cttagaaggt acaataaggc tatttaatcc agagcttaga
     7501 aagcaaattc ctggaatttt agaacgtata gcaaaatcta cagcagaagc atatagagca
     7561 gatgctgaat tagagtatgg gtacttaaca ccagcagtta taaatgacaa agaatgttca
     7621 aagatagcta cagatgcagc aattaagcta tttggagaag actgtataac actatttgaa
     7681 aaagtaacag gagcagaaga tttagctgag tttatgaata tagcacctgg tgcactagca
     7741 tttgtaggag ctagaaatga gtcaaaaggt gcttgttatc ctcatcatca tggatgcttt
     7801 aatatagatg aagatgctct agaaattgga actgctttat atgtgcaata tgcagtggat
     7861 ttcttaaata aataatttta atatactata aagaaaaagc agagactaag tttatatctc
     7921 tgctttttta ttgtgtatat tttttataaa tattgactat ataaaatata tatgatagca
     7981 taaagaaaat gataatcaat atcaattaaa aagaaggtga tttatttgaa taatgtaata
     8041 tttgcatttt tgataacatt attggctggt ttatctactg gtataggaag ttgtatagca
     8101 ttttttgcta aaaaaacaaa tactaagttt ctttctataa gtttaggatt ttctgctggt
     8161 gttatgattt atgtttctat gatagaaatt tttcctaaag cacaagatgc tttgacaaaa
     8221 tctatgggag agaaattagg aagttggtca actgtaatag ctttttttat aggtatagct
     8281 atcattgctg ttattgataa gctaattcca caagaagaaa atccacatga aataaagaaa
     8341 atggagaata tagaagaaaa gaatatcaaa aaaaataagt ctcttttaag aacaggtata
     8401 tttacagcaa tggctatagc aattcacaat tttcctgaag gattagctac atttatatca
     8461 gctcttgatg atgttacaat agctattccg atagcaattg caattgcaat acacaatata
     8521 ccagaaggta tatctgtatc tgtaccagtt tattatgcca caggagataa aaagaaagct
     8581 ttttattatt cctttttatc tggtatgtct gagcctttag gagcgattat tggatatgta
     8641 cttcttagaa attttttaaa tgatataact cttggaattg tatttgcaat tgtaggagga
     8701 attatggtat tcatatcact agatgaatta ttgccatcag ctagagagta cggggaacat
     8761 catttatcta tttatggatt gattgcagga atgggtgtta tggctattag tttattacta
     8821 tttaaatagc tttttaaata tatgattgta acctaaaatt agtatcaaaa tgatataatt
     8881 atttaaatag atttttaagc tatttaaaaa tatcattttg gagaatgagt gctatggaaa
     8941 agaaaataaa attcaaattt gactggtata gttcacctga taagatagag aaatggttag
     9001 aagatattga agctaaagga tataatttat ataagataaa taaatttgga ggaatatttt
     9061 attttcataa gggaagtcca agaaaaataa aatacactgt tgattactac tatgatgtag
     9121 agaataatta ttttgatgaa cataaatcaa atggctggaa tcttgtattt aggagtattg
     9181 gtagttttat gggtacatct gcatggtata tatggtcgaa agaatacaaa gacatacctc
     9241 ctaaattata tagtgattct gataaaacaa ataaagtaaa gtcaatttta ttaatgaata
     9301 tgtttatatt ctttatgtta gtagtaatat ttatatattt attgaatatt gaaataagaa
     9361 atttatatag tggttatgga gcatctgtaa ctgctattgt actacagatt atattgttga
     9421 ttttatatgc agtacaatta tataggagat taatgtatta tttaaggctt aaaaagttta
     9481 aataaataaa agaaggtgtc ctaaaattat ttttttaata ttttaagaca tcttcttttg
     9541 tcagtaagta ctgtttttaa ttatagtaat ttctgtcttt aaattgaaat atcaaataat
     9601 agttgtgtgg gattgagtat tgataaaaat aatttgaaaa tgttatccta aagtatcaga
     9661 tatacaaaat tatctttaaa tatattataa atattttaat tgtattaact agaaattatg
     9721 taagggggca ttttatgatt aagatagcag tttgtgaaga tgaaaaagaa acacaacttt
     9781 taatagaaga ttaccttgag aatatattaa aagatataag tatagaatat gagatacaaa
     9841 agtatatatc tggagaagag ttactggaaa gtaatttaaa agatatagat atattacttc
     9901 ttgatataaa aatggaaaaa ttaaatggaa tggatactgc aagaaagatt agagaagtag
     9961 acaatgaaat ggaaataata tttgtaactt cattaataga ttatgtgcaa gaaggttatg
    10021 aggtaagagc ttataggtat ttactaaagc caatagagtt ggaagaacta aaaaaacatg
    10081 tgctaacttg tatcaaagat attgaaataa ataaggaaag tcatattaca ataaaaaata
    10141 agtctaatac atataagatt tatttaaatg aaataaaata tatagaagtt caaaaaaaag
    10201 atatgctaat acacacaata aacaagaatt ttgatataaa atatagttta ggcaaaatag
    10261 aaaaagaatt aaatccatat aaatttataa gatgccataa aagttttata gtaaatttaa
    10321 gatatgttga aaatataaag cctaatgctg caatactgga aagtggagaa gaggtaccta
    10381 ttagtagata tagatataaa gaagttaagg agaaattctt aaaatttctt ggtgatacaa
    10441 tatgctaaat tttatgaatg agctattagt agttttatct atatttatac agttttctat
    10501 aatgaacgga gttttaattt ataaaaagga tagtaaaaaa aatgaaaaaa tagtattcta
    10561 tattgtcttg tttcttattt atattataat tcagataagt agattattta atggaggaaa
    10621 ttactttagt aatataaact atttatttta tatattagca tttattgctt ggggaggata
    10681 ctataattat ttttataaga tgggttatat taagtattcc attttctttt atctatttag
    10741 tgtatattat aaatgtttgg atgctgttat atatagaata ctattttttg caattacagg
    10801 taatgttatt tcatctaaag aatctttatt agtacctcac ttagaatcta taaaaattca
    10861 gatatatagt aatttaataa ttattcttat atatcttata tttagctttt ttagaaagtc
    10921 tataaaatta tttagccaag ataagagaaa ttatgtatat ttattatttg ccttattagt
    10981 taatacaatg aatatgcttg taaattatgt attaaaaaaa ttagaacggt ttggatcatt
    11041 acattcagaa ggatactatt ttgataattt tgttaatcca aaattggtag gagctagtag
    11101 tatatttctt atattattgt ttaaagaaat aataaaagag aatagattaa aatctcaagc
    11161 tgaattaata aaaaataagt tagatatgca atatgcgcat tatttaagta ttcaagaatc
    11221 tcatatgcga gttaagaaac tatatcacga tataaataat cacatttatt gtatagacaa
    11281 ccttagaaac aatagcaaag aaattaatga atatgttaac aatttaaaag atgaaataaa
    11341 aacatttaaa tatatctata atacaggaaa tatgatatta gacattataa tcaacgaaaa
    11401 gagtgaaata tgtttaaaaa aaggaattaa atttacttgc tctataaatt tttcaaaagt
    11461 aaattttgta aaacctatag atgtgtcaag catattttct aatattttgg ataatgcaat
    11521 agaagcatgt gataaaatag tggatgaaaa tattaataaa tatattagaa ttaaaggaac
    11581 tataacaagg tcattttttg tacttaaatg tgaaaatagt aaactaaatc agcttacttt
    11641 taataaaaat atattgttaa caaacaaaat ggataagttc gtacatggaa taggtattca
    11701 gagtattaaa tcttctttac aaaaatataa tggagaatta ttatttgaaa atagtatcga
    11761 taaatttata ctaaatatat atataccatt ggaccaagat actgacagtt ggggcgatta
    11821 agttaccagt tgggacatat gtattgatac aaaaaaataa ggatgttatc ttttaataac
    11881 aaaagataac atccttattt ctgtattaag gagcttataa ttttactaat agtaagttat
    11941 aagaattgga taggaaggtg atatgtattg aataataaat ctttaatata aagaaaatat
    12001 aaacatattc tagtgcaatg aataatactt tagagcggtt agtgtgatgg gaggattgtg
    12061 tatgaaagta aaaaggaata taaatagagt ggcaatcatt gtggctttaa atcttagctt
    12121 agcattgatg gtagggtgta tgtctcctgt atctgataag aacttaagta gctcagaaca
    12181 aattagtaaa gattcattga aagaacaagt tgaagataaa aaagatgaat ctatcgagct
    12241 atttaataat tactcacaaa agattaatga tgcgaaaggt agagaagaag taaaaaaaga
    12301 tttatctcaa agattttcta aagctataat agacagtggt tatagtatta ctgactcaaa
    12361 atcagatggt agctttgtag tatctataaa tggtgctgaa aaaaatattc gaagtttatt
    12421 ctattctatg tctgaagacg ataaagaagg aaaagtaaca atagatattc tctgtgctaa
    12481 agagtatcca gaagatgata agttatctga gagtgataaa tatattaaat ttatatacaa
    12541 tttatttaag tcattgacta atacgaactt aactgagaaa gaaatttttt ctatggttga
    12601 agaagacttt aataagggag taggtgttgt agagcttcct tatatgaagg atatacatgt
    12661 tgaagttaac aaagttaaac aatcaactaa ggttttaaaa ctaagtctag tatacaaatt
    12721 tgatatttca aatcattagt atattagaaa taactataac aatatagaaa atatatcata
    12781 gaaaattttt ttagatttta taaatttaac aaaaaaagta ttaatatata ggtgttcaag
    12841 gtagatgaaa aaaaataagt tattttgctt ccattttatg ctaaaggaca gtataatata
    12901 attaaagtat gttataacat acaaaaatgt agtatatatt atccaaaggg ggattttaaa
    12961 tgagtcaaac taatagaata gaagcattta gacaagaata tattaattct aaacctatga
    13021 tatgttgtga aagggctaga atttttacag aatcacataa aaaaactgag ggagaagcta
    13081 tctgtataag aagagctaaa gcttttttgg agacatgtaa agaacttcct ataaaaatct
    13141 ttgaaaatga attaatagta ggtacagcag gaaaatttag aagaacaggg atattgacac
    13201 cagagttttc ttggcaatgg gtagacaaag aaatggatac ttttgataaa agaactcaag
    13261 acccatatgt aatatcaaaa gaacaaatag aatttataag aaaagaaata tttccatatt
    13321 ggaagggaaa atctttggaa gaagttttct tagcaagaat cccagaagat acagctaaga
    13381 tattagtaga tacaggaata atagataatg attctaagtg gagacaggca gttggtgaag
    13441 taacaccaga ttatcaagat atattgtttg taaaaggata taaagggatt aaggaagatg
    13501 cagataaaaa aattaaagaa ttagatatat cagtttcaga aaatattgaa aagatagatt
    13561 tttacaaatc tgtttctata gttgcacagg gtattatgac attggcacaa agatattcaa
    13621 atcttgcaaa agagatgagt aagcaagaaa cagatgaaaa gagaaagtta gaacttataa
    13681 aaatttcaga gatatgtatg aatgtaccag caaaccctcc aactaatttt tatgaagcaa
    13741 tacaatttgt atggtttgtt caattaggtg gtattctatc agaaaatcca ttggcattaa
    13801 atttaggtag atttgaccaa tatatgtatc catattatga aaatgatgct agagaaggta
    13861 aaataactga atcagaagct caagaattga tagaagcact ttggattaag ttatcagaat
    13921 gggtatggac tatatcagca aatacagcaa attactttgc aggatataat caattccaaa
    13981 atcttactgt tggtggtaaa aagagaaatg gtactgatgg aacgaatgat atctcatata
    14041 tgtgtttaaa ggctacagaa agtgtaaaaa ctcaccagcc aggattaagt gttagagtga
    14101 gtcaaggagc accagacaat ttcgtaatgg cagttgctaa attggtaaaa caaggaactg
    14161 gtttccctgc tatacacagc gatagtgcag gggcacaaat gttattacaa gatggatatg
    14221 atgcagaaga tgctagagat tggagtaact gtggttgtgt agttcctcat tttagaaaaa
    14281 caggacagtg gacttcagcc gtaaatatca attttgcagc agctttagaa tatgctatga
    14341 atgaaggtaa gagtagatta actggagaaa aaatggggtt agatacaaaa aatatcacag
    14401 aatttacttc ttttgaagaa cttaaggatg agtttttaaa gcaattagca tatcttgtaa
    14461 aaagttctgt tataggaact actgttgctc aacaaattca taaagaaatg gtaccaagac
    14521 catttttatc tacttgtgta gatggatgtt tggataaggg agttgattta agtaaaggag
    14581 gagcaaaata taatataggt cctgtattaa ctggtatagg gttaggtgta gtttcaaatt
    14641 cattggcagc tataaaaaaa ttagtatttg aggataaggt gactacgcta gaagaattaa
    14701 caaaagcact aaataatgat tgggaaggtt atgaagaatt aagaaaactt gcgttggatg
    14761 ttcctaagta tggaaatgat aatgattatg tagattcatt agcaattgaa gtttctgatt
    14821 tttattatac tgaaactaga aaatacaagg atatttttgg ctctaaattt aatagtgcat
    14881 ttatgggtat atcaaactat gtaccaactg gaaaaatcgt aggtgcaact ccttgtggaa
    14941 gaaaagcgac aaaacctttg acagagggag tttctccatt tgttggtact gatacaacaa
    15001 gtccactagc agcaatgaaa tctgcatcaa aaataaatca tgatgttcat actggtggaa
    15061 cacttctaaa tttaagactg aatcaagatt tggtggaaac tgaaagagga cttagaaatc
    15121 taacatctat gattaaatca tattttgctt taggaggatt ccatgtacaa tttaatacga
    15181 tatcaaatga tactctatta aaagctcaag aaaatccaga agaatataaa gatttattag
    15241 tgagggttgc tggatatagt actcaatttg ttaatttatc aagagaaatg caagatgcta
    15301 taatagctag aaattcacat agcaattttt aattatgagg tagattatga gtaaaaaagg
    15361 tagagttgta aaagtacaac atttttcagt aaatgatgga gatggaataa ggactactat
    15421 atttttagaa ggatgtaaat taaaatgtaa gtggtgttct aatccagatt cctggagtaa
    15481 catagtaaaa ttaggtgtta tgaaagataa atgtgtatcc tgtaatagat gtatagatgt
    15541 ttgtcctcaa aatataagtt cactatttga tagagcgcaa ataaataata aatgtgattt
    15601 atgtggagag tgtatcaagg tatgtcttaa agatgccatt tgtataatga ccgaagaaat
    15661 gagtgttgaa gaaatagtag aggaagttga aaaagatttt atatttttct ttgaatctaa
    15721 tggagggata actttttctg gaggtgaacc tacacttcag attgattttt taagagaatt
    15781 agttgatatt ttttatgata aaggcataaa tattgcaatt gaaacttgtg gatattttga
    15841 ttggaataag gtaaatgatg tatttgagaa aatagaccac atatttgtag atataaaatc
    15901 tatggatgat aatattcata aagaatatac aggagtaagt aataaaatta tattagataa
    15961 tatatgtagg ctttcaaagt tgaataaatc tatggttata agagtgccaa ttatttatgg
    16021 ggtaaatgat agtgaagaaa atataagaaa tactgcatta tttgtcaaac aaaatgttcc
    16081 aggaggcaag atggagttac ttccatatca taaatttgga atcgataaat ataaggcatt
    16141 agggcttgaa gattatatat atgaatttga tgaaatttgt aacaatcata tgttaaaatt
    16201 aaaagagata gtagaactta caggagtaaa aatcatagaa tacaaatagc tatcaggagg
    16261 attttaacat ggataataaa tttcctaatg tgggagaaaa tttaaggtta ttgagacaag
    16321 aaatgggcat aagtttagac aaagcttcaa agatgacagg tgtcagtaaa gcaatgcttg
    16381 gacagattga gagaggtgaa tcaagtccga cagtatctac tttatggaaa atttcatctg
    16441 gatttaaaat aaattttact acactattaa atgaaaatac aaatacctat gaggttataa
    16501 agaaagaaga agtagagcct attgttgagc aaaaagggaa catgaagtta tatccaatat
    16561 acccttttag ccctcaaaga aggtttgaac tttttataat tgagcttgag gaaaactgta
    16621 ctcatgtttc atctacacat agtcatgttt tagaagagta tgtactcgta atagaaggta
    16681 aacttgacct taatgtggat gggaaaactt atattttaga gtctggacat tctattaggt
    16741 ttgatggaac tttagagcat atatataaaa atttaaataa aggaaaaact atatttcaca
    16801 atatagaggt ttatagataa ttttatagtt atccttttct aaaataaatg aaaattttag
    16861 aaaaggataa cttattttta taatgtcgaa aagatttata tgtatgtgat gatgttattg
    16921 tttaaaaaaa aacataaaag cattataata atattaacaa aatagaataa agaggaagag
    16981 aatatgatta
//
LOCUS       DAEMHZ010000087        16625 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000087 DAEMHZ010000000
VERSION     DAEMHZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.087"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            446..1123
                     /locus_tag="KPK72_002554"
     CDS             446..1123
                     /locus_tag="KPK72_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489758.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1195..3210
                     /locus_tag="KPK72_002555"
     CDS             1195..3210
                     /locus_tag="KPK72_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2489759.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3260..3940
                     /locus_tag="KPK72_002556"
     CDS             3260..3940
                     /locus_tag="KPK72_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489760.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4458..4904
                     /locus_tag="KPK72_002557"
                     /pseudo
     CDS             4458..4904
                     /locus_tag="KPK72_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5285..6202
                     /gene="pstC"
                     /locus_tag="KPK72_002558"
     CDS             5285..6202
                     /gene="pstC"
                     /locus_tag="KPK72_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG2489761.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            6204..7028
                     /gene="pstA"
                     /locus_tag="KPK72_002559"
     CDS             6204..7028
                     /gene="pstA"
                     /locus_tag="KPK72_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG2489762.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            7047..7811
                     /gene="pstB"
                     /locus_tag="KPK72_002560"
     CDS             7047..7811
                     /gene="pstB"
                     /locus_tag="KPK72_002560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG2489763.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7839..8492
                     /gene="phoU"
                     /locus_tag="KPK72_002561"
     CDS             7839..8492
                     /gene="phoU"
                     /locus_tag="KPK72_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG2489764.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8650..9519
                     /locus_tag="KPK72_002562"
     CDS             8650..9519
                     /locus_tag="KPK72_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2489765.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9606..11042
                     /locus_tag="KPK72_002563"
     CDS             9606..11042
                     /locus_tag="KPK72_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG2489766.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            11090..11917
                     /locus_tag="KPK72_002564"
     CDS             11090..11917
                     /locus_tag="KPK72_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2489767.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    12218..12480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12556..15222
                     /locus_tag="KPK72_002565"
     CDS             12556..15222
                     /locus_tag="KPK72_002565"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG2489768.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            15646..16356
                     /gene="rgaR"
                     /locus_tag="KPK72_002566"
     CDS             15646..16356
                     /gene="rgaR"
                     /locus_tag="KPK72_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG2489769.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         6601 a         1703 c         2960 g         5361 t
ORIGIN      
        1 aagggaaatt tttttaaaag tataatacta ttataataag aaaataaagt ttttataaac
       61 attgagatgc agaatataaa ccttttgtaa caatatattt accaaaataa tacaatttat
      121 tttatattat aaccatataa atcatataaa tcaatactca aactagccaa tagacaagtt
      181 tctagaaaaa taataaattt atacgtgaaa atgactttaa gctccattct ccaacaatcg
      241 gagactttaa ttagtattta ataatgaatc ttaatgttta atacaatatt caagaattat
      301 tcaaaaatac ttaagtatta tagtctagtt tgtaaagata ttataaaata ataataccag
      361 ttatatatga aaataaaaca ttataatttg taataactat aaaacttgga attataatta
      421 aaataccttt gaatggagtg gtgtaatgta taatttactg gttgtagatg atgatgttga
      481 tattttaaac ataaatcaaa tttatttttc taatataggt tttaatgtat atacatcaga
      541 gactgctaaa gatgctatgg atatagtaga gagtatagtt ttagactgta taattttgga
      601 tatttcattg ccagatatag atggatataa aatgtgcaat gatataaaag aaaaggttaa
      661 catacctata atatttttat ctaattatga gcatgaagaa gaaaaagttc ggggattttt
      721 agctgggggg gatgattata taacaaaacc ttatagttta aaagaacttg aacttagaat
      781 atatgctcgt atgaggcagt acaaaaatat tacatcagtt cctaatgtgt taaagttttc
      841 agcgttagta ataaatgtaa attcaagaaa agtaactttt gatggagaaa gtatagattt
      901 aactactatg gaatttgaaa tattattgtt tctagctgaa aacaaagaac aagtattttc
      961 gaagatagag atatataacc aagtttggaa aatgcctgat attggagatc aacatacagt
     1021 gcaggttcat attgcacaga tgagaaaaaa aattaattct ttaagcaggg agcatcaata
     1081 cattcaaacc gtatggggaa aaggatataa gtttgtacca taagtgagat actgtaatta
     1141 ataaatagtt cttgttttat ttaatactac tatgaaagag atgtggttga tacaatgaaa
     1201 aagtataata tactaaaagg aactctcatt gtattgatag tctctctgtt ggcaataaca
     1261 ggattaaatc ttctgtataa atatgataat aaatatacat ataagtctat tagagcaagt
     1321 aatggaattt taactataga taatgatact ttcactaaag gtaaacttgt ctttttgata
     1381 gatgactggg agttttataa tcataaaata tttaaaccga atgattttac agagcaagat
     1441 ataaaaccag aatatgtttt tataggtcaa tatccagatt tttctatgag aaaggggact
     1501 cagtcaccat atgggaatgc tacttatcgt atgagaataa aaaatgaagg caaggataag
     1561 ctactaagtt tagagcttcc tgagattttt tccgcaagtg aagtatggat aaatggagaa
     1621 atggtttcta aattaggaga tgtaggaaca gaaaaatata gacccaaaat taaaaattca
     1681 gtagtaagtt ttattgcaaa gaaaaataca gaaattataa tcaatgtatc gaatttttca
     1741 cattattata gtggtttgta ttatccgcct gcattaggag aaacaaagga cataagtaat
     1801 atgatgtttt atcgattact tttttatagt ataatttgct tcacaacttt tgcaatagct
     1861 atattttcat tgtctgtatg gtttctttca aacaaaagta aaatttactt atattttgga
     1921 tgtatgtgtg ttttctttgc catccatgta tcctatccct ttgtacattt gttaggactg
     1981 cctttggtaa gctttacata tgcggttgaa gatttgtcat attttatggt agttttatgt
     2041 attatttgta taaatggaag tatatccaaa atagaagaaa ataaagtgta cagattttta
     2101 attttaccat taggaattac aatgagtatc atatctatag tgataccaat tattcttctt
     2161 ccatatgata tgtactttgt caacatatat gggaagttaa tagacatata taaatatatt
     2221 gtatttatat atatattaat gtcttcgctg gtttcaatat ataaaaagac atgctctgag
     2281 catataagtt cttatatttt agtttcagta aacactgttt ttggaattag catattattt
     2341 gatgcagtta cgagtaatag atttgagcct atatttacag ggtggcaaac agaatattgt
     2401 gggtttgtaa ttgttatttt attttctgtt atgatgataa acaggaatag aataataata
     2461 aaagagaatg aaaaacttac aaggaatctt gaacatgaag ttgaagtgag aactaatgaa
     2521 cttacaacat tgttaaatga acgtaaaaga ttcttggcag atgtagcaca tgatttaaag
     2581 gcacctgttt cggctataca agcatttata gatttgataa aggttggtaa tattcatgta
     2641 gatgaagaga caagaaccta tctttatgct ataaatcaaa agtcaaatga agtacaaaat
     2701 agagtacgta gtttgcaaga atttacatca caagataaaa gcattggtat tagtgaaaaa
     2761 atatgtctga ataattttat taaagagata tatgaaaata atttgccaga tacacaagca
     2821 tgtggtgtaa actttgaaac ttatgtattg gagaaaaaaa tatatgcaaa gataaataaa
     2881 gagatgctta caagagcatt tgagaattta atttataatg ctttgacctt tactccattt
     2941 gatggaaaga taattttatc aatggataaa gaagatggat ttgcaattat taaaatttct
     3001 gataatggaa aaggtatacc tccagaaaat ataccaaagg tatttgacag attctttaca
     3061 gaaagagaca ataatgagcc aaaagggcaa gggttagggc tatatattgt caaatccatt
     3121 ataagagagc atggtggaga aatatttgta gattcagaac taggaaaagg aactgttttt
     3181 acaataaaat taagtctatg ttttttataa taatagctta tttaatataa aagaataatg
     3241 atatttggga gtgattataa tgcaaactaa gatattgata atagatggag acaaggataa
     3301 ttgccagaag ctaaaaggat ttctggagga aaaaggaata agtatagatt tagcatataa
     3361 ttgtgaagaa gctattggaa aaatatttag caataaatat gatttaatat ttttagaaat
     3421 aattctaagt gatggtgatg gatggactct ttgtaaaaaa attagaaatg taactacttg
     3481 tccaattgtt tatatgactt atataaatga agaccaaagc attttaaatg ctttaaattc
     3541 tggtggagac gattacttaa taaagcctct aaatttagaa atattatacg caaaagtaaa
     3601 agctatttta agaagaatga actcatatgt aaataataat gaaaataata caaaattaga
     3661 ggaatataat agaactagtg gagtaataaa gttagaagat aaaataataa aattgactcc
     3721 aacagaaaat aagttgttaa acttttttat agagaatcct gaaaagactt tgactactaa
     3781 agagatatat gaacatgtat ggatgaatga atacttagag gataactata gtgtggtagt
     3841 tgcagtgaat ggattaagaa agaaaataga gaaagattat aaaaatcctc agaaaattat
     3901 aacaataaga ggagcaggat attattttaa taaagaataa taaatatatt aatatcatta
     3961 ataattgtta taaatgtgaa atactaacaa tatctaaact aaattattat aaaatagctg
     4021 actgtaattt aattgataaa gcctattctt ctagactttg ccagttaaat tacagtcagt
     4081 tttattatat aaaaaaatgc gtaatggaac tattatttta tagattatat ttgaaaagat
     4141 agataaattg tatatctaaa tctatatatt atttttttac ctttaatata tattaaataa
     4201 taaaatagtt aaataataag attaagaatt tcttaataaa tccagaagat gctttcgatt
     4261 atataatatt ttatattttt atattattaa ttatatatgt tgcaattgct gcacctactt
     4321 tttctggtga gtatcagaca caatcagata gcatccttcg ttgtacgaaa catggacata
     4381 ttcgtctagc gtttactaaa atattagctc tattttctat atttataata atgtttgtta
     4441 tatgtatttc tatctatttg gcaatttctg atttagcttt tggtactgaa tgtttgaaaa
     4501 cctcatttca aatgttgttt tctgtcatta gtctagtaaa tataaattta ctacaagcac
     4561 agattcttat agctattggt ggtctaattt caatacttgc aatggttagt ttaacattat
     4621 ttttatctgc aaaatgtaag gattctctta cttctatgct gattgctttt tctatttgct
     4681 taattcctat gtgaataggt gttagttgga ttggatacct attaccgcca gggggaattg
     4741 gttttaagat tagtttatta tatcaaatga catcatttaa tttcttacac ttaggagata
     4801 tgagtttttg gacaccacac attatactgt ttttctcagt gatagaaata cctattttta
     4861 tatttttggc tattcgtacc tattgcaaac atcaagtatc gtaacaattt gatggaaata
     4921 atagtgatat aaactagagg atacaataat tatatatgta gtataaaagt taaatttagt
     4981 taacaaatct taatagcaga tataggtaat aaattataca taaactgtat tattatctat
     5041 atctgctatt ttaatataga gaaacaacag tatattgtaa agtaaatgtc aaatatagtt
     5101 ttaaaagtaa aattaatgtt aaaaagtaaa tataatttta cacttgctta acttaatcct
     5161 aatatagatt taacttaaaa aaagtagaat aaaagtacag gaaatttgat gaagacgaat
     5221 tataaaaaat taaattttga atacagaacg aaaattgata agtatatttt aatgggaggg
     5281 tattatgata gtaaaatcta gattagtagg tttagaaaaa aatgacaata atggcaataa
     5341 gacaaaatac gtaattgaaa aagtagcaaa aaatattttt ctacttagtg ctttagtagc
     5401 agttgcaagt ttacttttga taataggatt cgtattttat aaaggattaa gaccatttat
     5461 atttgaagga tattcattta tagacttttt aactggtgca gattgggttc cttctgccaa
     5521 taaatttgga atatcatcta tgatagtagc atcaatattt gcaacacttg gagcacttgt
     5581 tataggagta cctgttggta tattaacctc tgtatttata gcagaagtag cacctaaaag
     5641 attagcaaaa ataatgtcac cagcagttga acttttagca ggtatacctt cagtattata
     5701 tggtgtattt ggtcttgcag tgatagttcc aactatacaa ggtatattta atcttccaaa
     5761 aggtcaaagt ttgttagcaa tagtaatagt attagcagta atgatgttac caacaataat
     5821 atctgtatca gaaacagcaa taagagcagt accaaaagca tacaaagaag gttcattagc
     5881 acttggtgca tcaaaaatag aaacaatatt caaagttgta ttacctgctg caaaatctgg
     5941 agtattagcc gcagtagtgt taggagtagg aagagcttta ggtgaaacaa tggcagtaat
     6001 acttgttgca ggcaactcac cagttatgcc aactgcaata acagatagtg taagaccact
     6061 tacaactaat atagcacttg aaatgggata cgcatttggt acacatcaag aaatgctatt
     6121 tgcaactgga gtagtattgt ttacatttat attaatttta aacttagttt taaataaact
     6181 ttcaaataag gcggtaaatt aagatgagaa agttcaaaga gaatctattg aagtcattag
     6241 tatatctatc agcactattt acaatagtat cattagtagt tatagtggga tttatattta
     6301 taaagggaat tggcaacatg aattttaatt tcttatttag caattattca gcatcaggtg
     6361 atggtggtat acttccaatg ataattacaa cattatatac agtagtagtt tctatagttg
     6421 tagcaactcc tataggaata ttggctgcta tatatttaca agaatatgct aaaaaaggaa
     6481 aagcagttac agctataaga tttgcaactg aaagtttatc agggatacca tctatagtat
     6541 atggattatt tggaggaatt ttctttgtag ttacattaaa aatgggatat tccatagttg
     6601 caggttcact tacagttgca ataataatat taccagtaat aattcgtaca acagaagaag
     6661 ctttaaaaac agttcctcaa gcttatagag aatcatcatt ggcattagga gctacaaaat
     6721 ttcaaacttt atataaggta gtacttccaa gtgcaatacc aggaatttta tctggagtaa
     6781 tcttatcagt tggtcgtata attggagaat cagctgcaat attacttaca gcaggtactg
     6841 tagccaagat gccaggtgga atatttgata gtgcaagaac attaactgta cactcatact
     6901 tactgactaa agagagtgga gatatagcaa cagcagccag tataggaatt gttttaatag
     6961 taattgtatt agcactaaac atgctagcaa gatttgttgc taagaaatta aataaagcta
     7021 attattaaat ttaagaggtg tgaataatgg aattaatcga taagataaaa atgagtgtaa
     7081 aagatttgga tttattttat ggtgataagc aagcattaaa gaaaataaat atggatataa
     7141 aagagaataa ggttactgct ttaataggtc catctggatg cggaaaatca acttttataa
     7201 gaacgttaaa tagaatgaat gacttaatcg aagatgtaac aattaaagga aacatttctg
     7261 ttgatggaga ggacatatat acctcagatg atgttataaa tcttcgtact aaagtaggaa
     7321 tggtatttca aaagccaaat ccatttccta tgagtatata tgataatgtg gcatatggtc
     7381 ctcgtacaca tggactaaga gataagaagc aattagataa aattgtagaa gaaagcttaa
     7441 aaggtgcagc tatttgggat gaggttaaag acaggcttaa atcatctgca ctaggacttt
     7501 ctggtggtca acaacagcgt atatgtatag caagagcaat tgctatgaga ccagaagtta
     7561 ttcttatgga tgaacctaca tctgccttag acccaatttc aacattaaaa gtagaggaac
     7621 ttatagagga tttaaaaaaa gactacacaa tagtcatagt tacacataat atgcaacaag
     7681 ctgctcgtat atcagatgaa acagcttttt tcttaaatgg agaggttata gaatttagcg
     7741 atacaaaaac tatgtttacc actcctgtag ataaaagaac agaagactat ataacaggta
     7801 gatttggtta aaatatataa ctataaagga gttagacaat ggtcaataat agtttagata
     7861 taagtattga tactttaaaa gaacaaacag tgcttatgat ggaaaaatgt gaagaagcaa
     7921 tagaaaagtc tgtgacttgt atgatagaga aggatttaga gggtgcaaga aaagtagtag
     7981 aagatgatga agaaataaat aaattaagag aagaaataag agataagagt atagagctaa
     8041 tagtattaaa gcaaccaatg gcaagggact taagatttat ttattcatta ggtaatatat
     8101 ctatagagct ggaaagaata ggagattacg cagcaaatat agctagagaa atgttgaaga
     8161 taggtggaga ggcttatata aaagagctaa tagatattcc taagatggca aaagaatgta
     8221 agtctatgat gtctcagact agagaagctt taatcttatt tgatgcaagt atagcttatg
     8281 atgttggaac taaagatgat attgtagatg gattgtatga acaagtacaa attgattgtt
     8341 taaaaataat gcatgaaaac tcaaatacca tagaccaagg ggtaaaactt ctacttatag
     8401 gaagatacct tgaaagaatt ggagaccata taacaaatgt atgtgagaag ataatttttg
     8461 caattgaagg taaaatggtt gagataggat aaaaaactgc tttaaagcag tttttttatt
     8521 gtctaaatta agaaagaatt gataaaaaaa tatgattaat gggtaaattt aaaaaattgt
     8581 gatatactag gaacatatta aatatgagga tgcgtatttt atataaggtg ctttttagga
     8641 ggtggaactt tgaaacttca aaagatgggt atgaggacta taaaaacagg aattagtgtc
     8701 actttatgta tgattcttgg tgactattta gtagagaata tgttttattc tctggtagca
     8761 tgtataattt ctgttcaaga tacagtaaaa ggttctttaa agacgggaat caatagagtg
     8821 gaaggtacta ttttaggtgg tataatagga ttcatatttg ccatcattaa agctggagac
     8881 ccatttttat gtggtctagg agtaatgtgc tcaatatatt tatgcaatct gtttaaaata
     8941 aagtcagtag tagttgcctg tgtaaccttt ctggctatac atctaggtgt aggaaatagt
     9001 gaccctgtgt attattcttt acatagggtg ttagatacat ctgtgggtgt tttgtttgga
     9061 gtacttgtta attattatgt tgctagacca aattatctaa atagtgttat agatgagttt
     9121 aaaaagatag aagaactttc aatgttgctt attgaagata aaattcttag aaaagaaaat
     9181 ttaaatgtag agaaatttga agaagaagta gaaaaattag aagaaatata ttcaaagctt
     9241 attgatgagt tggattatag tttgagtgaa actaatatag aaaaaattga gaatgcctta
     9301 aggatatgta gagaaataca ttttcatatg cagtctatag agttacttga aaataaactt
     9361 tatttgaaag agcatacata taaatacctt aagaaaacat ataatgtgga taagctagac
     9421 tggaatatag atgaagaaaa aagccctgta ttcaactatc atctaatgaa aataatgatt
     9481 gaaatagagt tgttgagaaa actaaataaa tcatattaat aatagtagtt aaataataaa
     9541 taaaaaatag tatctttgta aatattaaac atatataagt ttaatttatg gaggtactat
     9601 tttttatgaa tactagaaat gacaattcta aaatactaga aatatataaa atgaagcttt
     9661 ttggcgaact tgtaaaagca gtgtggaatg atgacataga ggatattgaa aacttaccta
     9721 ataaaatttt aaatgaaaat ttaaaggata atttaaataa ttattttagt ggaaaatttg
     9781 ataaagatac tatggtaaat ttaattaggg taactatggg attggatgtg acagaagata
     9841 cagataaaaa tttaaaagag tcactacatg aagctgtaaa tttagatgaa gtcaaaagac
     9901 caataatatc tgtaatatca gatgcttgca aatattgtga tgaaaataat aaggaagatg
     9961 agtcatgtaa gataagaagt aagcatatta attgtaatga ggaagatgta tgtagtgctt
    10021 gtggtgattg tgtatcaaga tgtaagttgg gtgcaatttc agataaaata gaatttatac
    10081 caatgataaa attgttaaaa gatataaaga gccctgtata tgcaatagtt gcaccagctt
    10141 ttgtaggaca gtttgggaaa gatgtaagcc ctggtaaatt aagaactgca ttaaaaaaga
    10201 taggatttga agatatgata gaagttgctt tggcagcaga tatgcttact gcaaaagaag
    10261 catatgatta ttatgagcat atgaaaaagg acgaagaagg atgctttata actagttgtt
    10321 gctgtcctat ttgggttagt ttgatacaaa atagttttcc tcagatattg gaaaatgtat
    10381 caccatccgt ttctcctatg attgcttgtg ggagagctgt aaaaatttta aatccagatg
    10441 ctaaagttgt attcataggt ccttgtatgg caaaaaagaa agaagcaatg ttagaagata
    10501 taaaagatgc agttgatttt gtgcttacat ttaaagagtt gcaagaaata tttgatgcat
    10561 tagagttaaa tccagcaata atggatgaga gtaatagagt agaggcgtcc ctttcaggta
    10621 gagtttatgc tagaacagga ggtgtaagta aagcagttaa gttatcagtt agaagaattg
    10681 acaaaaatat aaaatttgaa gaaaaggcat ttgaaggaac aaaagagtgc atggacggtc
    10741 taaagaaagt tctaaataaa gaaatagaag ctacatttat agaaggaatg gggtgtgtag
    10801 gaggctgtgt aggaggtccg aagcgtattt taagtgtgga agaaggtact aagaatgttg
    10861 atgaatatag tgaaagtacg aatatggaaa ctccatttga taatttaaat gtattacaat
    10921 ttttaacttc tttgggaatc aaaagagtag aatctttagg agaaaaagaa gaagaaaagg
    10981 ttaaaaaaat atttagtaga gatataaaaa gtaataagaa agagaataaa tataaaaaat
    11041 aactttttaa gttttaaact tatgctttta taaaagggag atatggggga tgaaaattaa
    11101 agataataag gctgtttttc atgtatttat aaggatattt attatagctg ttttaatttt
    11161 aactggaaca ttattggagg aaaaaattaa taatgttcca gttgaaaata taagttcaaa
    11221 aggtgaaaat acactggaac tactttctca aagtaatact ttaggagaaa tcatatacaa
    11281 agcatctcaa gaagaaacaa aaggtccagg ggctacttct gaaaaagtag catacataac
    11341 aatagatgat ggtccatcaa aatatacaaa tgcactactt gatattttaa aagaaaatga
    11401 tgtcaaagcg acatttttca tgcttaatca gaatatgaaa aaccacaagg aagaagtaag
    11461 gagagtcttg gaagaagaaa actcaatagg ttttcatagt gtaagtcatg atatacataa
    11521 gttatataaa agcccagaag taacagttgg ggaatttaat acatgcaata atactctaca
    11581 agaaataact ggtcagagtt caaaattaat aagattacca tacggaagta aaccatatat
    11641 gccagaaggt tcatataata aacttatggc aaatgattat ttaatttggg actggaattt
    11701 ggatacacag gattggaagt cttctacaag tcagatagta agtaatatat tatattatgg
    11761 aagaaaaaga gatgaattag tagtattaat tcatgagaaa gagcaaagtt taaatgcttt
    11821 aaataatatt ataagaatct taaaagagag aggatataca atattgccaa ttacagaaaa
    11881 tataacacct aaaaatttct ggcaagaaaa cttataataa atttaaatat agtaaaagat
    11941 aaagtaaatt atttataaaa taaaacatat aatataaaaa atacgaataa gaggtatact
    12001 gtaaggataa tatagtataa aattaatgag aaagcattaa aattaaaata aaataatctt
    12061 gcaattattg acattataag acagaataac tgtaaaaaca tattttaaaa tataacaact
    12121 atataaaaga gaataaatat aattaagaaa attcagaaaa aataaaataa aaataattga
    12181 caaagagaat aatatttgta tataataata tgtaatatta aagactgaga agaagaggag
    12241 taagtaaata ctactaaaag agaggaaaat tcactggctg taagattttc taagtataag
    12301 atttactgaa tgtagctttg gagtttctaa actgaactat aagtaagttt agacggttag
    12361 aaccgttaaa ttctttgaga gtcaaagttg ataaaataac attaaccaat aatttaatta
    12421 tatgttaatg tattttaagc tttggaatta aggtggtaac gcgagctttt cgtccttttt
    12481 aaagaggatg aagagctctt ttttatttct aaagatattt aaaaaattta tttataaaat
    12541 acaggaggca ggacaatgga aaatacgaat ttatcaaaaa cttataaccc aaaagatttt
    12601 gaggctaggc tatataaaaa atggatggat gaagggtatt ttaaatctaa accaaatcca
    12661 gataaaaaac catttacaat aatgatgcca ccaccaaata taacagggca attgcatatg
    12721 ggacacgctc ttgaccatac acttcaagat atattaatca gatggaaacg tatggatgga
    12781 tatgaagctt tttggcttcc aggaactgac catgcttcta tagcaacaga agttaaagtt
    12841 gttgaaagaa taaaaaaaca agaaggcaag acaaagtatg aaataggtag agaagaattt
    12901 ttaaagagag cctgggaatg gaaagatgaa tttggaggaa aaatatctaa tcagcttaaa
    12961 caactaggtg attcttgtga ttgggataaa gaaagattta ctatggatga agggtgtaat
    13021 gaagcagtta tagaattttt tgtaagccta tatgaaaaag gtcacatata cagaggaaat
    13081 agaataataa actggtgccc agattgcaaa actactttat cagatgcaga agttgaacat
    13141 gaagaacacg acggaaactt ttatcatata aagtacccac taaaagatag tgaagacttt
    13201 ttagaaatag ctacaactag acctgagaca atgataggag atactggtat agcagtaaat
    13261 ccagaagatg atagatataa acatttaata ggaaaaacag caatacttcc attagtagga
    13321 agagaacttc caatagttgc agatagttat gttgatttag agtttggaac tggtgcagtt
    13381 aagatgactc cagcacatga ccctaatgat tttgaagtag gattaagaca taatcttgag
    13441 cagttaaata ctatgaatga agatggaact atgaatgaag tttgtggaaa atatgaaggt
    13501 atggatagat ttgagtgtag aaaagctatc gtagcagact taaaagaaca aggttaccta
    13561 attaagataa aagagcataa tcataatgta ggaacttgtt atagatgcca tacagtagtt
    13621 gagcctagat tatcagagca atggtttgtt aagatggaag aacttgcaaa accagctata
    13681 gatattctta aaaaaggaga attagaattt gttccagata aatttgataa aacttattta
    13741 caatggctag aaaatataag agattggtgt atatcaagac aattatggtg gggtcatcaa
    13801 ataccagctt attattgtca agagtgtgga gaaatagtag ttgctagaaa aatgccagat
    13861 aagtgtccta aatgtggaag cactcatttt aaacaagatg aagatgcctt agatacttgg
    13921 ttcagttctg cattatggcc tttctctact ctaggatggc caaataagac tgaagcttta
    13981 gattattatt atccaacaag tgtacttgtt acaggatacg atataatatt cttctgggta
    14041 gttagaatgg catttgcagg tatgttctgt atgaatgaaa agccatttga ccatgttttg
    14101 gttcatggat tagtaagaga ttctcaaggt agaaaaatga gtaaatcttt aggaaatggt
    14161 atcgacccat tggagataat cgagcaatat ggagctgatg ccctaagatt tacattaaca
    14221 actggaaatt ccccaggaaa tgatatgaga ttctatatgg aaagagtaga atttgctaga
    14281 aactttgcaa ataaattgtg gaatgcatca agatttgtat ttatgaatat tgatgaagat
    14341 ataattaaaa acatgactag agaaagtgtt aaagaggact taactctagc tgataaatgg
    14401 ataatatcaa gagcaaataa tattgtaaaa gaagcaacta ataacatgga taagtttgat
    14461 ttaggaatag cacttcaaaa gatatacgat tttacttggt cagaatactg tgactggtat
    14521 attgaaatgg ttaaacctag attatatgga gaagatgcaa atgctaaatc tgcagcatta
    14581 tatacattaa cttatgttct tgaaaaaata ttaaaattat tacatccata catgccattt
    14641 ataacagagg aaatatatac tcatcttcct actgttgaag gatgtataat agtatctgaa
    14701 tggccaaaat ataatgaaga agataacatg gctgaagaag aagatatgat gaacttattg
    14761 atggaaggta taagaagtat aagaaatgtg agagcagaaa tgaatgtacc accttctaag
    14821 aaagctaaat tgataataat cccaagtgaa gaaaaaatag aagctattga gcttggtaaa
    14881 gactatttta taactctagc atctgcttca aatgtagaga tagcaaaaga taaatctaat
    14941 gttccagaag atgcagttgg agtagttatt gatggtgttg aaatatttat acctcttaat
    15001 gaattagttg attttgaaaa agaaattgag agattgtcta aagaaaagaa aaagttagaa
    15061 ggcgaaataa agagagttaa tggaaagctt gcaaatcaag gtttcttagc aaaagctcct
    15121 gaaagtttaa ttgaagaaga aaaagcaaaa aaagaaaaat ttgaagagat gattaaatct
    15181 gttgaggaaa gattaacaaa tttagaatct aaaataaaat aaatataaga attagtaaaa
    15241 catacaaaaa gtatatattc tatacttttt gtatgttttt ctttatatat atacaaatat
    15301 gatgttataa ttctttacag atgtgttata tttttatttt atgttgtgaa tatatgaatt
    15361 ttatgtctta aaatttataa gcaataaaaa tttgatatat tatgatgttt aatatagggt
    15421 aataaataca tattatattt attttactat atttttttac ataatatatc tagaaaagta
    15481 taggaattta tcgtaattta agtttaaata ttaaagagaa tttctataag tagtttaatc
    15541 gacaatttaa atttataaag aaatttaaat ttgaaagcta gtgagtacag tttggaaatt
    15601 gaaataaagt aaactaaatg atattttaga tttggaggtg atattatgtt cagggtagtt
    15661 atatgtgatg acgaaaaaat acaaagaagc atactaaaag agtttacaga aaaacttttg
    15721 tctgaaagat gtctaaatta tcaaatactg gaattttcct gtggagaaga tttaatatcc
    15781 aaatacccag aaaaaattga tattatattt ttagatatcc aaatgaaaga tatcaatgga
    15841 attgaggtgg ctaaaaaaat aagaaagttt gatgaaaaag tagaaattat atttacaacg
    15901 gctttttcag agtatgcccc ccaaggttat gaagttcgtg cttatagata tttagtaaag
    15961 cctatagagt atagtaactt tgcaatgggt gtaaatcttt gtattgataa ccttcaaaga
    16021 aagaaggagc gttatatagt tttaaatagt aaaaaaggat ttaatagaat actgatagat
    16081 tctattttat atgttgaaac tgtaaagaga gatttagttg tacatacaat aggtagagat
    16141 tataaagcta atatgagcat gaaacaagca gaaaaattac tgtgtgaaaa tggctttttt
    16201 agatgtcata caagttttat tgttaattta agaagaatag aagatcttag ggataatacg
    16261 attactatta atgaacaatt tatacctata agtaagtata gacttaaaga tttaaaagta
    16321 gcattaacaa gcttgttatg tgatataatg tattaatttt aaaaaggtaa ttgaaaatat
    16381 cgtaaaaaag atttaatgtc atcacagggc tattgattga aactgtctta atatataaaa
    16441 tgttatttat attaagacag ttttattatg caaaatagca gtttatagat ttttaatatg
    16501 gtttaatatg tatatgtaga gtttccatta taatagcatg attattaatt agtagcttga
    16561 ttatcaatat actattattg atttttatat gcattatatg tatttttaga atatgattac
    16621 ctttt
//
LOCUS       DAEMHZ010000088        16469 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000088 DAEMHZ010000000
VERSION     DAEMHZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.088"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..854
                     /locus_tag="KPK72_002567"
     CDS             165..854
                     /locus_tag="KPK72_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489770.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            854..1771
                     /locus_tag="KPK72_002568"
     CDS             854..1771
                     /locus_tag="KPK72_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2489771.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1844..2770
                     /locus_tag="KPK72_002569"
     CDS             1844..2770
                     /locus_tag="KPK72_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489772.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2767..3447
                     /locus_tag="KPK72_002570"
     CDS             2767..3447
                     /locus_tag="KPK72_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489773.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTLVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            3545..3880
                     /locus_tag="KPK72_002571"
                     /pseudo
     CDS             3545..3880
                     /locus_tag="KPK72_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            4864..5514
                     /locus_tag="KPK72_002572"
     CDS             4864..5514
                     /locus_tag="KPK72_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2489774.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            5852..7918
                     /locus_tag="KPK72_002573"
     CDS             5852..7918
                     /locus_tag="KPK72_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2489775.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVIQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            7935..9890
                     /locus_tag="KPK72_002574"
     CDS             7935..9890
                     /locus_tag="KPK72_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2489776.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCAAPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIISHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            9906..11096
                     /locus_tag="KPK72_002575"
     CDS             9906..11096
                     /locus_tag="KPK72_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG2489777.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(11551..11733)
                     /locus_tag="KPK72_002576"
     CDS             complement(11551..11733)
                     /locus_tag="KPK72_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489778.1"
                     /translation="MITKDNTLKYEAFNIQYCALNNITPKYHPPFTIIEYPNYFKKEN
                     IRNGKPWIYKIKNTNF"
     gene            complement(11946..12129)
                     /locus_tag="KPK72_002577"
                     /pseudo
     CDS             complement(11946..12129)
                     /locus_tag="KPK72_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12246..12620
                     /locus_tag="KPK72_002578"
     CDS             12246..12620
                     /locus_tag="KPK72_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2489779.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            12622..13479
                     /locus_tag="KPK72_002579"
     CDS             12622..13479
                     /locus_tag="KPK72_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489780.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVIKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            13482..14120
                     /locus_tag="KPK72_002580"
     CDS             13482..14120
                     /locus_tag="KPK72_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2489781.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            15139..15873
                     /locus_tag="KPK72_002581"
     CDS             15139..15873
                     /locus_tag="KPK72_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2489782.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT         6238 a         1835 c         2778 g         5618 t
ORIGIN      
        1 tactatattt taattatttt ttcaataaag cttattgaaa agaactttaa cttagaagag
       61 tatcaccaaa ttgcagatac aatatcataa gcagttattt attgacaacc ctttttaata
      121 aatatatcat ttgaagtata ctgacgaaaa ggagttgaat aataatggac atcttactgg
      181 ttgaagatga taagaatatt cgagaaatgg tatacgattt tctgattagt gagaattact
      241 ctgttaaaac tgccattgat ggaaatacag ctattgaaat atttaaaaat tattcatttg
      301 atattatttt actggacttg atgttaccaa agtctagtgg ttttgaagta ttaaaatata
      361 tacgaagtgt gagtgttgtt cctgttatta ttactactgc gaaagatagt gacacagata
      421 aaatgctagg gcttaatctt ggtgcagatg attatattac aaagcctttt tcaatcgtag
      481 aattgcttgc aagaattaaa gctaatatta gacgtgcaac agtgtatagc agttcaagaa
      541 actctcaaga tatggttatt cggtataagg atttgattat aaacatatca aaatatgtag
      601 tttcaaagaa tggaattgac ttaaacttaa cacatacaga atttgaaatt gttaaattac
      661 tagcgactaa tcaaggacgt gctttttcaa aggaacaatt atacaacgct atttggaaag
      721 aaccttatta tggaaatgaa aatgtgctaa atactcatat taacaggtta agaaataaat
      781 tgtctgatgg tatagaaaaa aataattata ttaaaactct ttggggaatt ggctacaaaa
      841 tggaggaaga ataatggagt tatttttagt tgtaatttcg attgtactat ttgctctcta
      901 cttaaaagaa cgaaataaat caaagaaaat ggaaagtgat tataagtata tcaattcaag
      961 acttcgtgac ataacaaatg atgatgaaat taattatatt ttagtaccat ctgatatttc
     1021 gattgtaaaa gaaacagcag tagatataaa taaacttgtt gaaaagtttt acagcaggca
     1081 aattaattat aatagaagtg aaaaagcgat attgcaaata tttacaaaca tatcacacga
     1141 tttacgaact cccatcacag tattaaaggg ttatattgaa atgctatatc ttcaaagtca
     1201 aaaagaagaa ttatcttctt caatgagagc aactgtggaa aaaatgcaaa caaattcaaa
     1261 tgagcttgtt aattcaataa ataacctgtt taatatggca aaaatccaat ctggagatat
     1321 gatattaaat cttcaaaaat ccaatctaac acaattatgt catgagatta ttttagagtt
     1381 ttatgatttg ctagataaag aaaactttaa tgttgaagtt aatattgaag atagacctat
     1441 atatgcaaat gttgacacag aagcattaca acgtatactc aaaaatttaa ttgataatgc
     1501 tataaaatat ggttcagatg gtagattttt agggatttca ctatatgaaa aggaaaaaca
     1561 tgtttttata gatattgagg ataaaggttc aggtatttct gaaactgaaa aagagcatat
     1621 tttcacaaga gcttatactg ctgacagaaa aaaaggcaat ggtttaggtt tggctatttc
     1681 tcaagggctt gtaaactcta tgggaggttc aatttatgta aatagtagac ctaatgtaaa
     1741 aacagttttt acggtaaaat tgaaaagtta gaaaaaagtt agatttaaaa gagagaaaag
     1801 ataattttat tttgtatact gtttttagaa aggagggagt atgatgaact atatcataga
     1861 gacccatcaa ttaaagaaaa tatataaaga taaagctgtt gttaatgctg ttaatattca
     1921 tgtgaaaaag ggagaaattt atggatttgt tggaccaaac ggggcaggta aaagtacagt
     1981 aatgaaaatg ttgctaaatc ttgttaatcc aaattctgga gaaatagtta tgttcggtaa
     2041 gaaagtggca gaaactgatt ttgaaatatt aaaaaagatt ggtactatta ttgaaaatcc
     2101 gtatttatat gagaatttga ctgctaaaca gaatcttgat ttacactgtg aatatatggg
     2161 ttattataac aaagaacata tatcagaggt attggaatgt gtcggattgt caaagcaatc
     2221 aaacaagaaa gtttcaaaat attctttagg gatgaaacaa cgtcttgcaa tagctagagc
     2281 aattcttact aaaccagaat tattgattct tgatgaacct ataaatgcac tcgacccaga
     2341 ggggataaga gaaatgcgag aattattccg taagttaaat acagattatg gaataactat
     2401 atttatttct agtcatattc tttcagaggt agaacaaatt gcggacacta ttggaattat
     2461 tcaagatggt aaacttatta aagaaatttc tatgtcagat atacacaaat atcaaacaga
     2521 ttatgttgaa gtagatgtag ataatgtgga gcttgctgga tacttgttag aaaaagaatt
     2581 tggaataaaa gactttaaaa ttacttcgga aagttgtatt gaaatatatg atttacgaaa
     2641 agatgtaaaa gaaatatcca aaatatttat tcaaaatcaa attggtataa atagcattgg
     2701 acgtaaacaa agttctttag aagattactt cttccaaact acaggtacag gaggtaaaga
     2761 aacactatga tacatttaat gaaactagag ctaaaaaaag ttgggctaaa aagatatttt
     2821 ttattttcaa tgctggggat tttcttatgt atgttttttg tctttactgg attgaatgat
     2881 aaatctactt caatatatga ttatgatgtt acatttagga caatcagttt atttttctgt
     2941 ttttactata ttatattatt tgctgtgttg aatgtggcat atataataaa tgaatataca
     3001 aataaaacaa tcttgatttt attctcttat ccacttgacc gtaaaaaact tattttatca
     3061 aagttacttt tgattacgac tcttatgata tcatcaatga tagttggata tatctgttgt
     3121 tttacattcg ttgttgtgtt ggataagtat tttaatttag ttattggaga ttttcatatt
     3181 tcaattttat cttattggat accaggagca attaaaagta ttattgtatt ttgctcatta
     3241 ggtatatgga cattagttgt tggaatgata agaaaatcaa tacctgttac ccttgtcagt
     3301 tcaattattt ttatcaattt aagacaattt attttagctg gtacaaatac aactcaagac
     3361 agtttgattt ctgtttttat tatagttggt ataactgtga ttggtatata ttatatacta
     3421 aatcataaag ttactgaaat tgactagata ctgtggctag gaaggtagtt attaattgta
     3481 tcttcttggg ataataatga tttgctatta tatcagctat tattaatatt attagagttt
     3541 taataatatt gcacaaccat ttgaagttga gccaaagctt gatgaattaa ttgaatggta
     3601 ctatagtcag agtgaagtat ctattaaagt tataactgaa tttcattata gatttgaatt
     3661 gatacatcca tttcaagatg gtaatgggcg cataggtaga tttgttatgc taaaacagat
     3721 gttagaaaat aatcttccta taaaaattgt atcttgggat agtgaggaat tgtatagaaa
     3781 ctccttaaat tcttgtagtt taggtaatta tgtcccttta atcgaatatt tatcaagttt
     3841 agaagatttt agagaagtat ataaaatgtt atggaaataa ttgttttcag caaaaaaagt
     3901 ttttatataa atatattttt atctatgcta tttaacgctg atttcttagt tattcttagc
     3961 ttggactata tccactattt taactatata aaatagtgga tatagtttaa ctatctcttt
     4021 ataactattt ttatgtatct tagttgtttc ttaatatggg gacagttcat aaaactaaaa
     4081 gtgattttta attttatcat ttataattta ccattacatc agaaaaatgc ttatcattag
     4141 aattttcata aaaggtacct atttttttca attaactaaa tcaattctat tagtcttaat
     4201 ctttcgtatt tcaaattgat aatataagaa aataaaaaat aaaaaatttt aaatttttta
     4261 tttattgcga aaaaaataaa aaggtgctat actttttgta acatattatt caaattatta
     4321 agagatagta aacatttata gtttaatttg tagtacacta gcatgatttg cagaacaatg
     4381 aattaatcta tagtataata gtttaattta aagtacaacg atttaatcta tagtacaata
     4441 acttgatttg aagtacaacg atttaatcta tagtacaata acttgatttg caatacaatg
     4501 aattaatcta tggtataata gtttaatttg aagtacaatg aattaatcta tagtataata
     4561 gtttaatttg aagtacaacg atttaatcta tagtataata gtttaatttg aagtacaatg
     4621 gtttaatcta tagtataata gtttaatttg aagtataacg atttaatcta tagtacaata
     4681 acttgatttg caatacaatg aattaatcta tagtataaga gcatgattta cagtacaata
     4741 atttaaattg tagtacaaaa gtactatcaa ataagaattt ctactttaaa aatattacta
     4801 aaagtaatta aaatccaata attcaatata atatggcaaa agaatagaaa taaggtgaga
     4861 gtagtgaaag taaagtcaaa tttagatgaa attttatatc ataagattat tgaaagtctt
     4921 attcgtggtg aatactctgt aggtcaaaaa atattactaa atgacctttg tgaaaaattt
     4981 gaagtaagca gaacaccagt tgtacaagca gtaaaaatgc taaataaaga tggagtgctt
     5041 actattatga caaatggtaa agtgtacgta ccagagtatg aatatgatat ggtaaaacaa
     5101 gtttgtgaaa cgagaacact gattgaaaca tatgctttag aaaaaatgat gcaagaagag
     5161 gaagaggttt ttcaacaaaa gctagatgta ataaaaagat actctgataa atgcgaggag
     5221 tactatcgtc aagaaaaatc tgtagaactt gcactagcag atttggaatt acataaagcc
     5281 attgtagaag gggcaaataa tgagatttta aatgatgtat atgttggtat acaaggaaga
     5341 tttatagtag tgaactattt aattcgacca ttaaaaaatc gtaattatga aggaacagta
     5401 caagaccatt ttgaaatatt aaaattcatt gaagacagag atacaaaaaa agcagtagat
     5461 aaactaagaa atcatattca aggaactatt aaaagattct gtgaagatga ataataagtt
     5521 ttgaagttac tatatatact tatctattaa gttgctaaat gtagtctaag tataatctaa
     5581 ttatagttta aacataatct aaatataatc taataaatat aatctaaata tagtgtaaat
     5641 aagtctaagt atgaattaaa tatagattaa gtatggacta agtatagatt aagtatagat
     5701 taagtatgaa ttaagcatga attaagcatg gattaaatat actttaaatt acaaatatta
     5761 caaggaagcc agaaggcttc ctaaaaaaat aacctttata aaatttttta ttttaaattt
     5821 aaaatttttt attttaaatt taaaataaaa aatgaaagag gtggtaggga gtttgaaaaa
     5881 aattactaaa gaaaggatag aaaatactat gaaaaagcta tttacgagtg aaacattttt
     5941 ggaatatcaa tttataagtg attgccaaat atcaccaaat ggaggatata cagcatttat
     6001 tgtaaaaaaa gctgatatca aagagaatgg ttacacaagc caagtttata tattaaacaa
     6061 taaaactggc gaattaaaac aaataacgtc tataaactct gttggagcat atgcctggga
     6121 agatgaaaat actatcttgt tccctgcttt gagaaatgaa aaagtaaaag aagctgtcaa
     6181 aaacggtaag caatgcatga gttactatgc tttatcttta aatggtggag aagcagaaga
     6241 actttttaga ctccctatca aaggtggtaa attgaatcct ataggaaaag gtttgtatgc
     6301 agttattgat tcttatgaca atgaccgacc tattgtagag ggattgccag aagaagaaca
     6361 acagaaaaaa ataaatgctt ataacaaaag acattatgaa catttcaaag aaattcctta
     6421 tgctgtcaat ggggagggat atattagcag aaaaagaaaa cgcctatatg tatacgacag
     6481 taatataaat gagttgaagg caattacagc acctatgttt aatgttgtag gtatgaaaat
     6541 aagtgatgga aaaatcttat atatagggca agagtttaaa gatgtaaaag gtttgaaaaa
     6601 tggggtctat gtatttgata caaacacaaa cacaaatgtc tgtatattag aaaaagataa
     6661 gtatattata aaaggttttg aattatatca aaatcaagtg atactaaacc taactgatgc
     6721 tttatcttat ggaaatggag aaaatggaga tttttatact attgatattg atacgaaaga
     6781 aatgaaactt ctttcagggc atcaacatca ttgtattgga aatacagtaa catctgatgt
     6841 taaaatgggt gctggtcaga caacaaaggt agatggagaa tatatttatt acacttctac
     6901 agtagatatg gactgcatta ttgaaagaat acatattcca acaggaaaac aggaaaaagt
     6961 aatacagaca ggttcagttg attttataga tgtaaaagat ggcaatattg tatgtgtggg
     7021 ttgtttagga aatggattac cagaagttta tacagtagag aatggaacat tgtgtaagaa
     7081 aacacattta aatgaccata ttttagagga atataaaatt tctgtgccag aatacataga
     7141 gtcaaaagga agttctaaat gggaaataca aggatacgta tataaaccag tagattatga
     7201 ggtggggaaa aaatatccag ctgtattggc gattcatgga ggaccaagat tgacatatgg
     7261 accatatttt atgcatgaga tacaggtatt tacaagtgca ggttactttg tattcttctg
     7321 taacccaagg gggtctgaag gtcgtggcaa tgcttttgca gatattagga aacagtttgg
     7381 agatattgat tatatagatt ttatggaatt tacagatact gtacttgaaa aatatacaga
     7441 cattgataaa acaaaattgg ctgttgaagg tggaagttat ggtggattta tgacgaattg
     7501 gataattggt catacaaacc gttttgctgt tgcttgtgct cagcgttcta ttgcaaactg
     7561 gagtggcatg gagggtacaa cagacattgg ttattatttc tgcaaaggac aaacaggtgc
     7621 ttctcatatg gaaaaccacg agcttcaatg gaaacagtca cctcttgctt atgctgataa
     7681 atgtgttaca ccgactttat ttttacatgg tgaaaaagac tatagatgct atatgcaaga
     7741 agcattccag atgttttcag cattgaaaat tcatgggtgt cctacaaaac tttgtctatt
     7801 tgaaggagaa aaccatgaac tgtctcgttc tggtagacca aaacaaaagc tacaaagatt
     7861 agttgaaatg ctagattggt tttcggtata tataaaaaaa gataacatag gacagtagaa
     7921 aggagaatat aaaaatggaa aaaataaaga ttgaaagttc agatattttt gaatatatat
     7981 tccctcatga tatttcttgt tcacctgatg gaaagcatat tgcttatatt atatcaaata
     8041 tcaacgagga aaaagactgc tatgaacatg atttatatgt gatggacatc aaaacagaaa
     8101 aacaaataca tatgactcaa acaaaagatg taacatcatt tagttggatt tctaatacag
     8161 aactactttt tacatctaaa agaaataagc caaaagcagg tacaacagat ttttacacta
     8221 tttctataga aggaggagaa gcaaaaaaag cattttctat tccaaaagct tgtagcgtac
     8281 ctgtatcact tggaaataaa ttgtggttat tgacaacaaa aaatccaacg gattctaaaa
     8341 aatcagaacc agatagggca gtagagggtg tagattattg gacatttaca gataaacctt
     8401 ttataagaga tggagaaaat ttttctcaaa gaagacgtgt aacattagaa ttatatcaag
     8461 aaggtgaaaa tataacaaaa gccattacac caaaattttg tgaagtagca ggtgtagacg
     8521 tttcttctga taaaaacaga attttatata ctggtcagat ttatgaagat tgtgcagctc
     8581 ctttttctgg actttgggag tatcatatag acagtggtga aaccaaagaa cttgtaccac
     8641 aaggaaagta ccaaataagt ttggcaaaat atattggcaa agataaagta atgttgcaag
     8701 cttcaacatt agaccgttct attacacaga atcatgatat ttttatactt gatttgagta
     8761 ctagtgaaat aaatatgatt gctagtccag atggtatgta tgctacattg ctggatgtag
     8821 atgcagtata tggtggtgga cgtagtaata aagttatagg agacaaattt attggtgcaa
     8881 gaatttgcag aactatgaca gaatttaatg agtttgatac aaagacaggt aatatacgta
     8941 tcatcacaaa agtagatgct tttacatctt ttgatattta cgacaacacc atgtacacag
     9001 taatgttgaa agattatgag ttagcagaaa tatatagcat tgatatgact acaggtacaa
     9061 tgaaaaaaat gactgctttt tctaaaccat atttagatac tcataaggtt tcattacctg
     9121 aaaaattgac gtttgtagca aaaaacaaag aagaagttga tggttttgtt ataccaccta
     9181 tagatgctaa agaaggcgaa aaatatcctg ctgtattgtt cattcatggt gggcctaaat
     9241 gggcatatgg ctatatgttt acacacttga agcagtgtgt aacatcaaag ggaatgtatg
     9301 tgatttattg taaccctcat ggtggtgacg gttatggtga aaaattctta gaaatggtag
     9361 aaagatgggg gtatgttgac tatgagcatc ttatggaatt tgtagatact tgtattgaaa
     9421 aatatcctgg gattgatgca gaccgcttag gggttgcagg gggaagttac ggtggttata
     9481 tgacaaactg gataattagt catacagacc gtttcaaagc agcagcttct caaagaggta
     9541 ttagtaactt gattacagct tctttaatta ttgactttgg ggatagaatt atgaagcaga
     9601 cttgtggaga taagacgcct tggaatcatg aggaggttct ttggaatcat tcacctatta
     9661 aatatgtgaa aaatgttaag acaccaacat tgttcttaca tagtgatagg gattatagat
     9721 gttttatggg ggatactttc caaatgttta cagcattaaa acaacttggt gtagatacag
     9781 aaatgtattt gtttcacgga gatacacatg gattgtctag aaatggaaga ccatctaata
     9841 gaattgcacg agcaaatgct attgtagatt ggtttgaaag atatttataa taagggggtt
     9901 attctatgag tgatatgtta ttatggttta gtgtcgtatt agttttattt gctattgggg
     9961 atttaattgc aagcaaaaca aaagcaaaag tatcagctgt atttgttaca ctacttttat
    10021 ttttgattct atttgtcaca aaagcaatac cagctgatat tattgaaaaa gcaggtatga
    10081 ccgcagcagc tagttggagt gttccaatga ttatgtttag tatgggaaca atgttgaatg
    10141 ttaaacaatt tatagacgaa tggagaacag tattgacagc atggcttgga attgtagcag
    10201 ttatagtctg tgtctcatta tgtattcctt tgtttggaaa atcaacagta ttgacaagca
    10261 taccagttat aaatggagct ttacctgcta ctactattat gacacaagca gcactagaaa
    10321 aaggactgac acttgcagca gctacagcaa cagttgtatt tgcaattcag aagtttattg
    10381 gtacaccaat agcttcaaga gcagcgttac aagaggcaaa ccgtctttta gtagaatatc
    10441 atgaagcaaa atcaaaaggc attgatttag caaatgttga tactgataag aaagaagcag
    10501 agaatacagg cacaaaagta aaacaagctt tctgtgaaaa atacgataaa tattattcta
    10561 caaatgtttg tatatttttt atagcactat tctcttatct agggtatgaa ctttcagaga
    10621 ttattcatgt aaattattct attgtatgtc ttgttgttgg tgttattgta acacgtattg
    10681 gtattgtgcc aaaagacatt ttggaaaaag gaaaaatcaa gggatttatc aacatggttg
    10741 tgtttgcagc tgttattcct tctttagcaa aagtttcttt gacagattta attagtcttt
    10801 ttgtaccaat tgtgggtatg tttgcagctt ctatcattgg tatattcctt atgatgaagg
    10861 tgttaccagg ttggaaaatt atcggttcta aacctcttgc ttttggagta gggttttgtc
    10921 agatgttggg attcccaact acatatttaa tttcaaatga agtgtgtaat gctgttggag
    10981 aaacggaaga agaaagagca tatttgatgt ctaagattat gcctaagctg gtagttggtg
    11041 gtatggcttg tatgatttct attgtggtag ctggtcttat ggtaccaatg ttgtagatag
    11101 gtttcaaagt taaaaaagtg gtgttaattt ataggagaag ctaaaaagta gtattaattt
    11161 ataaacgaat tttatattag ttaatttatt ataagaatat tttcttttta gcattataat
    11221 cttgatttta tcaagaaaca gattttttat ttgctattct aatagttaca gtttattttt
    11281 attttctaaa acaaaacttt aacttgattt atctgtaaat agggtattta ataatatttt
    11341 tggaaaatct ttagaaaaaa ttaaaaagag gagaaaaaat ggagtgcctc aaagtgaact
    11401 tttttagttc ataaggcact cttttttgta cgaaatcgaa tatattttca acgttttaaa
    11461 agtgagaaag agacttatct ctagtttgag atagccaatt ttaattataa aaattatttt
    11521 ttgttttatt tatcaaagag tattcgtgtt ttagaagtta gtatttttta ttttatatat
    11581 ccaaggtttt ccgtttctaa tgttttcttt tttaaaataa ttagggtatt caattatagt
    11641 aaaaggtgga tggtattttg gagtgatatt atttaaagca cagtattgaa tgttgaaagc
    11701 ctcatatttt agagtattat cttttgtaat caaaaaaatc ttctatactt atagcaaggt
    11761 tacttctgca aatattccat acataataat atcccaattt aaattttttt aatatagagt
    11821 ttacctctag tctacactct tgaataactc tttcctctat ccaaattctt ataaattcat
    11881 cccatttatt actatttatt tattattatc taaatttttt agtatgtttg taattagaaa
    11941 tggatttatt cctttatgtt ttttataaat agtttataac ttaatttatc atcaaacaaa
    12001 tcattcatac aggattactt tttcttttat atattcaatt cttactttta ataaatattt
    12061 aatacatcct atcttatgtg gttttaaaaa gtgtttatag ctatcaacaa tctctaatat
    12121 ttcattcata tttattttgt catagtaata ttttacatct tttatattga caaaaaagtg
    12181 ctctaagtgt atagtgtgta tataacaaca tcacactaaa aatgataaaa agggggtgat
    12241 aaaaaatgaa agtgctcatt tctaacacat cagataagcc attataccaa caaattaaag
    12301 agcaaattaa agaagcaata tttactgaag aactgaaaga aggggatgca atgccatcca
    12361 taagaaattt tgctaatgat ttaaaagtta gtattttgac tattagacga gtgtatgaag
    12421 aacttgaaaa tgaaggcttt cttacacgcc aagttggaat aggaacattt atttcaactg
    12481 gaaatttaga aatactaaga gactcaaaga gacgtatagt tgaacaaaaa atggcagata
    12541 tgattaagga tgcaaaagca ctaggaatta cacagataga gttacaagaa atgatggata
    12601 ttctatatga ggaggattaa tatggaaaat attttagaag tttataattt aaataagact
    12661 tataccgatt tttcattatc taatataagc tttgcattac cagaagattg tataaccgga
    12721 tttataggtg ttaatggagc aggaaaaaca acaacaatcc gttcaatatt ggatgtcata
    12781 aagcgtgata gtggaaaaat aaaaatcttt ggtaaagatt tcgatggaaa tgagaaggaa
    12841 ttaaaaaatc gaattggagt tgtatttgat gattcatatt tctatgagga gttgtcaatg
    12901 gcagaaatga aaagtattat tgcaccttca tatgataatt ggtgtgaaga agattatcaa
    12961 caaaatatga agcgttttaa tcttaaccca aaacaaaaga ttgatacact ttcaaaagga
    13021 atgaaaatga aatttgcttt agcattagca ttatcgcata aagctgagtt tttaattatg
    13081 gatgagccta caagtggctt agacccacta atccgtagtc agatacttga catactaaca
    13141 gaatatatga agaaatgtgg tcgtggtgtt ttcttttcca ctcatataac atctgattta
    13201 gacaaaatag cagatatgat tgttatgatt gacaatggac gtattatatt agagcgtgaa
    13261 aaagatactc tattggacga acatagaaaa gtaaaagggg atattcaatt tctaaatgat
    13321 gaaaccaaaa aattattttt aaatttagaa attacaggtt atggttttat agggattacc
    13381 tcaaaaatta atgaagtgca aaaaaatatg ccagatattt taatagagcg tcctacaatt
    13441 gaagatatta tgcttgcata tattgaaagg agagagtaat tatgttgatt catttagtta
    13501 aaaaagatat tctgattgta aaaaaatatg tattgttgat attatttatt gcaataggaa
    13561 tcccattatt tatattatgg cgtttacctc catttgcaca aatattggga tttgtactat
    13621 ccattatatt ttcagaattt atgctgtgcc agtatctatt attaaaggag agtcaatatt
    13681 caaaagcatc tgtattactt tgttcaactc cttatcctag acgtggctta gttcaatcta
    13741 agtatgtttt atttgcaatg gtatttatct attgtacatt agtttactgg attgaaaatt
    13801 tacttattcc acaaatagga acttttgact taacatatat tttgattgtt ttattaataa
    13861 gttctattat ttatggagtt tatatgccta ttcaatataa attaggatat gaaacaacaa
    13921 aattcttttt cgtggtgata atcatggctt catcatttgt attaccatta actatagctc
    13981 acagcaattt tgaaattcca tttatatatc agtatccatc catgatagtg aatattgtat
    14041 tgttcctttt aagtttaatt atactgttag cttcaatgtt tatttctatt aaaattttta
    14101 gtaataagga gcttgtataa ggtaaggagg aattttatat gccacattta ccagattggg
    14161 ctgttttact ttgtatctta cttccaatag tatataaaga ttgtcagata tttaaaagta
    14221 aaaaatagag tacatattta tattgccatt tttattgagt gaagttagat ggtatgaatt
    14281 taaaaggtta cttgaaaata aagttacttg gtgcgggaga gtaacatcat aatattatag
    14341 attatatgtt tcttgtgaat caatttgtat tttttaatag ataatatcca gtaattttat
    14401 tttccattga ttatttttca attatgagta tgattgtgtg tattgtatac catcttttaa
    14461 ggtgttttta aaaaattata tcgtcaaaat ataaatctaa ggaatcctat aaataaagct
    14521 ttttaatttt agaaaactaa taaaaagtag gaagttaata aaaagaagct gtctcaaaat
    14581 agagataagc ttctttttca ttgctgaaat gacgaaaata taattgattt catataaaaa
    14641 agagtgcttt atgaactatt tattttgaac attagataca cttgcactca atttagttac
    14701 tcctaagctt tatgaactaa aagtttattt tgaagcctat ttttattaaa agagatttta
    14761 tttttctaaa taatcatcta tatacagtat tgcgtttctt ttatttattc ataatttatt
    14821 ttcttattaa agtttttcta tattcatatt ttctatgtta ctttactttt aattttatac
    14881 gaatatacga attatcaaaa ataaaatagt tattagaaaa aaataaaagt aattgcaaag
    14941 tttgcaatta cttacttcta tattaattac tataccataa aataacaaaa aaatcaagtc
    15001 gagtaatcct tcaacttaag tgatatacta ggtattaata atttttaatt aataaaatat
    15061 aactagagga aaatctacat tttctcaatt tctttaatta aattgacctc taattaagta
    15121 attataagga gaaaaattat gaatttagca aagggattag atgttttaaa aatttcatca
    15181 aatgtattcg gtgaagataa agttatgtat attcctgtaa tctacacaga agatgatgca
    15241 acacttattg acacaggtct tccgggtcaa ggtgatttaa taattgatgc tcttaataaa
    15301 agtaacacga gctttgatag gttaaaaaat ataataatta ctcaccatga tatagaccat
    15361 ataggaaaca taaattatct aagagaaaaa tctaagaata atatcaaagt atatgcatac
    15421 aaaagtgaag tcagctatat aacaggagaa gaaactcctt ttaaacttta tatgttggaa
    15481 caaatggtag ataaaattga tgataagatg cttagtatgc taaatgttat gggacttgga
    15541 tttaaatcat catatactaa agtagatgtg tcattagata accatgaaaa attgaatcta
    15601 ggtgaggaaa tagaagttat acatacagga ggtcacacta gaggtcacat atgtctttat
    15661 ttaaaagagt ctaaagtact tattgctgga gatttgttac aggtagaaaa tggagaactt
    15721 aagcctgttg atgtaatgca cagcaacaaa caagagttaa aagatgctat taaaaatata
    15781 agtaattatg atatagaaac gatagttttt agtcatggtg gattatatca aagaaacata
    15841 attggaacat tgaaaaacct aattattgag tagtactata tgatttacaa taatttctta
    15901 aattacttaa aattatatct aaagatgtag aatcaaaatt aaatataagt aataagaatt
    15961 ttgctttaaa attatactta agtgttaagg gtgatacgta gtattacaag tattttagaa
    16021 tataattttt tatagtaatt ttaaagttct acaatacttc agttgaaaaa tatttaacag
    16081 catagtataa taggaaagaa aatattttaa ataaacttta gtatacaaag tgagtaaaaa
    16141 tatttaaatc atatatcata atgcagagtg aagaaaaata tttagataat gtaccataat
    16201 acaaagtgag taaaaatatt taaagcatgt ataataatgc aaagcgaaga aaaatattta
    16261 aataatgtac cataatgcag agtgagtaaa aatatttaaa taatgtttta taataataca
    16321 aaatgaataa gattatttaa agcatgtatt ataatataaa gtgagtaaaa atatttaaat
    16381 catgtatcat aatacaaagt aaagaagatt atttaaacca tatatcataa tgcaaagtga
    16441 agaaaaatgt ttaaattatg tatcataat
//
LOCUS       DAEMHZ010000089        16431 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000089 DAEMHZ010000000
VERSION     DAEMHZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.089"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(80..766)
                     /locus_tag="KPK72_002582"
     CDS             complement(80..766)
                     /locus_tag="KPK72_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG2489783.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(815..1348)
                     /locus_tag="KPK72_002583"
     CDS             complement(815..1348)
                     /locus_tag="KPK72_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2489784.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1392..2024)
                     /locus_tag="KPK72_002584"
     CDS             complement(1392..2024)
                     /locus_tag="KPK72_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG2489785.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2114..3193)
                     /locus_tag="KPK72_002585"
     CDS             complement(2114..3193)
                     /locus_tag="KPK72_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG2489786.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3326..4672)
                     /locus_tag="KPK72_002586"
     CDS             complement(3326..4672)
                     /locus_tag="KPK72_002586"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG2489787.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGTIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4741..6021)
                     /locus_tag="KPK72_002587"
     CDS             complement(4741..6021)
                     /locus_tag="KPK72_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG2489788.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6148..6408)
                     /gene="hfq"
                     /locus_tag="KPK72_002588"
     CDS             complement(6148..6408)
                     /gene="hfq"
                     /locus_tag="KPK72_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG2489789.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6522..7457)
                     /gene="miaA"
                     /locus_tag="KPK72_002589"
     CDS             complement(6522..7457)
                     /gene="miaA"
                     /locus_tag="KPK72_002589"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG2489790.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7495..9462)
                     /gene="mutL"
                     /locus_tag="KPK72_002590"
     CDS             complement(7495..9462)
                     /gene="mutL"
                     /locus_tag="KPK72_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG2489791.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYHLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9473..12316)
                     /gene="mutS"
                     /locus_tag="KPK72_002591"
     CDS             complement(9473..12316)
                     /gene="mutS"
                     /locus_tag="KPK72_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG2489792.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12479..13213)
                     /locus_tag="KPK72_002592"
     CDS             complement(12479..13213)
                     /locus_tag="KPK72_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489793.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13182..14195)
                     /locus_tag="KPK72_002593"
     CDS             complement(13182..14195)
                     /locus_tag="KPK72_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2489794.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14217..14987)
                     /locus_tag="KPK72_002594"
     CDS             complement(14217..14987)
                     /locus_tag="KPK72_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489795.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14962..15252)
                     /locus_tag="KPK72_002595"
     CDS             complement(14962..15252)
                     /locus_tag="KPK72_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG2489796.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15383..15482)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16102..16191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16244..16420
                     /locus_tag="KPK72_002596"
     CDS             16244..16420
                     /locus_tag="KPK72_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489797.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
BASE COUNT         5164 a         2828 c         1730 g         6709 t
ORIGIN      
        1 tttccttcta aataaaaaat ttgtctaaag tttctaaaaa atttagacaa atcttaggtt
       61 attactcttt aaattatttt taaattattc caaatacgcc aaataatatt ggtataaata
      121 tagctggtaa caaattggct acttttatat tcgttaattt aagtacattt agaccaagtc
      181 ctataattaa taaacttcca gctgctgtca tttctgtgac tacaggatca cttaaaaatc
      241 catttaaaaa tgatgctccc acacatataa taccttcata caaaaataca gatattgcag
      301 aaaacattac acctatacct aaagaagcag taaatataat tgaagctatt ccatctataa
      361 cagattttac aaataaagta tcatgacttc ctgtaagacc actttctaat gaaccaacta
      421 ctgccatggc tcctatacag aataataaac tagatgatat aaaaccctct gctatagaat
      481 cttttttatt tcctttagaa aatttacttt gtaaaaaagc acccaactta tttacccatt
      541 tgtcaatatc tattaattct cctatcagag cacctacagc tacggatatt attgtcacta
      601 atgtgttttt tccttctagt gctcctgata taccaatgta caatacacat aaggcaattc
      661 cattcattat agtatcgctt actctttgag gtaatcctcc ttttattata agacctacta
      721 tacatccacc tgcaattgct agactatttg ctattactcc aaacattttt tctcccctcg
      781 attagctaat tgaaatttat cctattatta aaacctaaac atctatactt tgtattggat
      841 attttaaata ttccaaaaat tcttcaaaac cttttctaga tatatttaca gttttagtat
      901 ttatgttagg atgaaaattt aagtaatctt ctttaattac atctttatct atgtaaagct
      961 caacttttct ttctgtatca tttattaatg aaaaaggatt tacacttcca ggaagaagtt
     1021 taagtacatt gtatagtctt tcaggtgaag caaacgataa tctagaactt cctatcttat
     1081 ctttaagtga gtttaaatca acttgttttt ctcctttaac tacaataaga taatacttat
     1141 ttcctttggc atttcttaaa aataaatttt tacattgaca cccctttgtt attccatcta
     1201 aaatgactaa ttgctctgct gtatatacag cttcatgctc tactacatca tattcaacat
     1261 ttaatttatc caaaatatca tatattttag tttcattcat gctagattca ttttcatctt
     1321 gttctttttt attatttaaa tcactcatac ttttctcctt aaacacttca tcataatatc
     1381 attgtgaggt attattctac tatagctatt gctcttactg gagctccatc agcatcatta
     1441 tacttaaatg gtgctgctat aaatagaaat ttttctggaa cattttctaa atttgtaaga
     1501 ttttctatga ttatttttcc tttttcaaat aaaatatgat gcacttcaaa tactgatgtg
     1561 tcatatctat ccactgatag catatctatt cctatgcctt ttatattagt attagctata
     1621 tagtttgcag cttcttttgt taatgtagga taacctacat agtactgctt tttatcccaa
     1681 aattttgacc aacctgattt aaatattata aaatcactat tttttatttc atcttcatat
     1741 ttttttagat attctaattc tatattctca tttccctttt caagttccaa tacataagct
     1801 ttcccaacaa aattttctat atccaactta tctagggtct cacctttagt atacatatgt
     1861 cttggtgaat ccatatgagt tccattgtga gaaaatacac taattaatgt ttcttgataa
     1921 ccattttctt caataatagc tactttcttg atatctggtc tattaggctc tgcatataca
     1981 ggcatgtcat tatgtgtcac atgagttaaa tcaaaaacct tcatctttat atacccccta
     2041 tgtttttatt ttctattata tacctaaatt cattttattt ctatcagatt ttattccatt
     2101 atataccata ctactatata ttttttataa acctcaaggg atttaccgaa aaatcttgga
     2161 aagaattatc agtaaatata acatcttcac ccaaaaaacg tttatacctt ccttcttctt
     2221 tctttaaagt aattctctgt ttttgtgaat gattattaaa aaccattatc ttaagaggag
     2281 gtttttttct attagtctgc actcttttta acatgtattt tatgtgcata tcagcttcag
     2341 gaaatgaata tccacaaaat attaaaaggt cagtatctct taaagttttt tctgtttcat
     2401 tccaaacatt acttaaaaat atgtttgaca tattcttaaa atatgttggt ggcactataa
     2461 taggttctgt taactcacca cactctaaac attttgtctc tgaactattt tcaataagct
     2521 tcatgacacc accttcatgt ggtgtaagag ttacactatt acaaatgggg caataaagcc
     2581 aattcaatga cccatgtatc ttatataatt tgattaatgg ttcttttggt tttctccaat
     2641 ttaactcttc tttaaaattt gtaaactcca caccataatc aagcataatt ggcaaatcat
     2701 tcttatctct taatccagta actgtattgt ctatatgtat atcataattt gcactaataa
     2761 aagtagtatc aagaagcaaa tcattttcca aaagattttc aactaaaagt ttatgatatt
     2821 tattatctgt tctaggtgta ttatgaattg cttcagccat tagcagtatt aaatattgtc
     2881 ttaaaaaacg tatactatca cttctgttat ttagattttc tagtccaaaa tttctaaatg
     2941 cttcttttct ttgttctgct aagtctaaaa gacctaaaac ttcttcaaag gtaggaaaat
     3001 ttgcattatc tatattatct tttactatat caatattgaa catctgttta aagaaaatat
     3061 acaattctct attcattttg gtattataat ttgtgggatt tagtttttta aaatattcgc
     3121 taaaaagttc attttgaata ggtagattct ctgctgctga agctcctgct cctaaaaata
     3181 ttgctatttt catatagttc ctcctaatat aaatattact cctattttta tatccattaa
     3241 atatccatat catttatttt atgatacaac aataaagctg tcttttacta gaaaaaatcc
     3301 tattacaaga cagccttata ttatattaaa tctttaataa ttcgtaaata gaaagagcat
     3361 atatttgagt agcttttata atatcctcta ctattaagta ttcattagct tggtgttctg
     3421 tttctggttt tcctggaaaa atagttccaa atgcaacaca attatctaaa gctcttgcgt
     3481 atgttgctcc tccagatgaa agtggtgttc catcaagacc agtttcttct tcatatactt
     3541 ttctaagtgt cttaacaaga agagtatctt ctggaacata gattgaatcc aagaaatcat
     3601 attcttcata atttaaattg tatttatctg tcattttctt taactctttt acaaaatcat
     3661 cctttttgta tgttactgga atccttacat ctattcctgc aaattctttt tcgttatcaa
     3721 tagttactct acctatattt actgttagtt tacctgatac atcatccttg caattaggaa
     3781 ttatattgtt tccattagca tcttcaccaa taacttctgc taaaaactta attatattag
     3841 aatctatacc tatattatta agagctatac aaagtctagc cactgcattt ataccagtat
     3901 cacttgctgc tgaatggaca ctttttccaa ttatacagat tttattacct tcaacagtat
     3961 attcaaaatt taatttatca agttcttttt ttaatttatc agagtatttt ccaatataga
     4021 ttgctttacc tggaactgca tttaaagcct ttccaactga caactctata tcacttttgc
     4081 tatcacaagt caaatgaact tgtaaaagac ctttctctgc atttgttatt gggaatctag
     4141 aatctggagt aaatccatag tttggtattt cttcattatt ttctttatat ttatttatac
     4201 atctccaaag attttcttca tcagccccaa aaatgaatct tatcctctta ttaaagtcta
     4261 catttaaatc cataagagct ttcactgcat aaattgctga tattgtaggt cctttatcat
     4321 cttgtgttcc tcttccatac aatttaccat cttctaaaac agcttcaaat ggagggtaat
     4381 tccagctttc taaatcacct tctggaacca catcaacgtg accaagtata cctatcattt
     4441 tttcaccttg tcctatttct gcatatccat aatatccatc tttgtatact gtcttaaatc
     4501 ctaaagattt acataagtct aatatacctc ttaaagcttt atctacattt tcaccaaatg
     4561 ggcaattttc agttgcttca gatataacac taggtatttt tacactttct tgtattgaag
     4621 aaatcatttc atcttgtaga gaatttactt tttctttaat ttgctctttc atattatcac
     4681 ccttcaattt tcatcataat atactaataa atattttgac cttttaaaaa aaaaatatgc
     4741 ttattttttc agttttttta taaattcaat cgcttttaaa gtccccattt ttgaatggtc
     4801 aaatgtaagt ccaccttgaa aataaacatt ataaggaggt cttattggag catctgcact
     4861 taattctata gaagaacctt gtataaatgc tcctgctgcc attatgactt cactttcgta
     4921 tccaggcatg tcccaaggaa caggttttac ataagaatcg actggtgcag cttcttgtat
     4981 accttcacaa aaacttatta cttcatcagc attattaagt ctaatacatt gaataatatc
     5041 acttcttaag tcatcatatt ttggtaatac atcatatcct aatttttcaa aagctcttga
     5101 gcaaaagata gctcccatta cagcctgaga tactatataa ggcgccaaga aaaaaccctg
     5161 aagtacattt ctagttgttc caaaagtaag accacattct tttcctattc caggagaagt
     5221 cattctataa gatataagct caatcaaatc ttttctacca gctatatatc cacctgtcaa
     5281 tgcaagccca ccaccaggat tttttattaa agagccagcc ataacatctg ctccaacatc
     5341 agtaggctct ttggtatcta aaaactcacc ataacaatta tcaaccatta ctatagcttc
     5401 tggtttaaca gactttatta cttctatagc ttcttttata tcactaattg aaagtgattt
     5461 tctccaagaa tatccttttg acctttgtat cataacgagt ttagttctat catttatttt
     5521 gtttttaatt ccttctaaat ctaagtttcc atcctctaaa aaatctacat catcatatgt
     5581 aacaccatac tctttcaatg aacctttttc ttctcttatg cctataactc cctctaaagt
     5641 atcatatggt ctaccagtaa cagataaaat ttcatctcct ggtctaacta taccttgtat
     5701 acataaagta agtgcatgtg taccatttac aattattggt cttacaagag catcttctgt
     5761 attaaatact ttagaataaa tttcctctat tttttcacga ccaatatcat tatatccata
     5821 tcctgtagtc cagttaaagt gcatatcact tagtttagat tcttgcatag cttttaaaac
     5881 tttatattga ttatattctc ttatttcttt tatttcttca aacttatctt ttatttcttc
     5941 cattatttct tgcgatagtt taaaagtatc atcatctatt ccatagtagt cttttaataa
     6001 ttcttttgtc tcattaagca ttttatcacc tgtctcttta ttattttatt taaacatgta
     6061 aaggagacta ttttactaaa acaaataaat tatagtttat aagttagtat aagtctcctc
     6121 tcaatcatct taaattaaat taattatcta tctgttgtta ttattattgt tgttttggtt
     6181 ttgatttgtt agattaatat actttccagg ttgtatagtt gatacagcat gtttataaat
     6241 catgttttgt tgtctattat ccccttctat taatattata taactatcaa acccctttac
     6301 aagcccttta acttgcactc cattaactaa atatatagta acaggtatcc tttctttcct
     6361 tgcattattt aaaaacaaat cttgtaaatt taaaactgta tttttcatta gatttgtacc
     6421 ctcccaagat taaattgaac ttctaaaata ttcctacctt atattctata taagttatga
     6481 caaattttca atattaagtt gtatacttaa aaatttatat attattttat agaatcttta
     6541 atatataaga ttatttcatt ttttaattcg tctatatttt catattggtc taaatcaaac
     6601 cattttgctg ttttatatct tttaaaccat gttatctgac gttttgcata ccttctagaa
     6661 tctcttttaa ttatttcaat agcttcttcg tatgtatatt ctccatccag atactttaat
     6721 atctctttat aacctatacc ttgcattgat atcatatctt ttttaaatcc catacttaaa
     6781 agttttttta cctcttcaac caatccattt ttaatcataa tatcaactct catatttatt
     6841 ctttgataca aatgttctct atctctattt agaactatta ttattggttg atattcttca
     6901 ttaaatttta aatcacttga aaaatcattc attttcttac cacttagaca aacttcaaga
     6961 gctcttatga cccttttagt attgttttta tggattctat tagcagattc ttcatcaagt
     7021 tcttttaact tattatgcat gtaatctatt cccttttcag caagttgttt ttgaagttct
     7081 tctcttaatt cattattggc atcagattgt gcaaaatcca tattatatat taaagagttt
     7141 aaataaagtc ctgtgcctcc agttactaaa acacattttt cctttttatt tatttcatgt
     7201 atatattttt ttgctctttg ttgaaactct gaaactgaaa atggatagtc aggcttaact
     7261 tcatctataa ggtaatgctt cactccttgc atctcttcct ctgtaacttt tgcacttcca
     7321 atatccatat attcatatat ttgcatagaa tcagctgaaa ttatctctgc attcatatct
     7381 tttgctaatt ttattgacaa atctgttttt ccaacagcag ttggacctgt aagtattata
     7441 agtggtattt ttttcataac attcctttcc atatatttta aatatccaaa tattttacat
     7501 aattctttta aacatttttt ctatttctgt cttagatatt tcgaccatta taggtcttcc
     7561 atgaggacat gtaaatggat tctcacactt ttctagctgt tccaataaac tttttatttc
     7621 aatatcgtat attttatcat ttgcttttat agcagaccta catgccatag atgcaaatct
     7681 ctctcctttt aaatcataat tacttgtaat ttcttctata ttatcaatta tttgaagtat
     7741 aaatttctcg gtttcaggga ctccaaatat agtaggaaca catcttacca ttatatgatt
     7801 attgccaaaa atttctagct caaaaccaaa tttcataaat aactccaagt tattttcaat
     7861 ttgaagcata tctacatttg atacttcaat aaccactggg tctaaaagta tttgcatatt
     7921 tatatcttgt ctatagaact tctccatata tctttcatat agtattcttt catgtgcagc
     7981 atgttggtcc aacaaataca tagaatcgtc tttgctcaaa attatataag tattaaatac
     8041 aactccaatt acagaatatc cgtataaaga aaacttgcgt tttgaactac acaagtattc
     8101 ttcttcactg tccttaatac tatctcctaa atgataattt ccttcatttt taattccatc
     8161 aacttgaaat tcttcttgta tattatcatt tgcactatct tcacttagat aattaatgtt
     8221 ttttacatca tcttctacac ttgcatttag gacttcactt actgattgaa attcatttat
     8281 tggtttttca cttgataaag taacaacttc aatgacttca tccttacctt tagtaatatt
     8341 actattttta ggagtagatt ctaataaatc attatttctt aatttataat ctgtactttt
     8401 ttctctactg ttaatagcga ttctaggttg tgttttttta tcactataag tagcatattt
     8461 tccaataaga tttgagtgta ttaatttaac ctttaaaaaa tctctaagtt ctatatacac
     8521 ttcttgttct ttttcaaatt taatttcaag tttattgggg tgtatattta catctataca
     8581 tgatgggtct acttctatat ttaagaagca cactgcatgt ttacctattg gaattataga
     8641 cttataactt tctgttattg catctatgat tattttactt tttacaaatc ttttatttat
     8701 atagatatgt tgtaagtttt tatttgaacg gtatatattg ttattaccta tatatccatt
     8761 cattttaaag tgattgcact taaactctac atctattata ttttccgtta tttcttttcc
     8821 atatatactt ctaatagtgt ttacaagctt tccatcccct ggagtattta gcatctgttt
     8881 attgttattt gtatatttaa attgaatgtt tggattacct attgcaagtt tatttatcaa
     8941 atcacttata tttatagttt ctgcatgagt agattttaaa aatttctgtc ttgctggtgt
     9001 attaaagaaa atatctttta taatgatggt agttccatta gtagaaccta ttggttcttt
     9061 cgatattatt tttccacctt caacatatat tttagttcct attatttcat ctttagtttt
     9121 agtcgtcatt tctaattttg atacagctga aatagatgcc aatgcttctc ctctaaaacc
     9181 aagagagtat aaatcataca aatcatcaat ttttttaatt ttactagtag catgtctcaa
     9241 aaatgatttt tcaacttcac ttgaaggtat tcctattcca ttatctgtaa tcttaattag
     9301 actttttcct ccatcaatta tatctatgga aattttattt gcacctgcat caatggagtt
     9361 ttcaataagt tcttttacta cagaagaagg tctttctaca acttcacctg ctgctatctt
     9421 atttatagtc aaatcatcta ggatgttaat tatatttttc atttttacac ctctaagatt
     9481 ttatttcttt tgcttttctt tgaagattat aaagagagtt tatagcatct aatggagtca
     9541 tatttaaaat atccagattt aaaatttctt ctgatagtat atctctattt acactatcaa
     9601 aagagatttg agttaaggct acttctttta cagaatcctc ttgattaatt ttctctttat
     9661 catcattttg cttcattaaa gcaacttcat catttaatgc tttaaattta gaatttaatt
     9721 tatcataatc cttttttata tgtttgtaat cttttactat aagttcatgt tctattttta
     9781 gagattcata tttattctta aaattacttt gatttacctt aactaattct tcgtccaaat
     9841 tactattaat tatattattg ttttctttat ctccatttat tgcaacactg ttgtaaacat
     9901 gatttttttc caagtcttta agtatatatt ttgctctttc tatgacttcg tctggtaatt
     9961 tagctaattt agcaacatat attccataac tcttgtctgc tccctgagga attatttttc
    10021 ttaaaaatat tattccttcc ccatcttctt tgactgctat tgaataattt ttaacctctt
    10081 taaactcttc ttctaaatca gtaagttcat gataatgtgt tgcaaataat gtcttacatc
    10141 taatattttt ttgaatatat tccactattg accatgctaa acttatacca tcataggtac
    10201 tagttccacg acctatttca tctaatataa ctaaacttct ctctgttgca ttttttaata
    10261 taagggatac ttcgttcatc tctaccataa aagtagattg accttgagat aaatcgtcac
    10321 tagctccaac tcttgtaaat attctatcta atattggaat atcagcagac tctgctggta
    10381 caaatgagcc tatatgtgcc ataagagcta ttatagcagt ttgtctcata tatgttgatt
    10441 tacctgacat attaggacca gttattatgt taataatgtt ttctccacga tttaaataag
    10501 tatcatttgg aacgaaattt tcctctccta ctatattttc gacaacagga tgtctaccat
    10561 ttcttatatc aagtttatta ttttcattta ttgctggttt tacatagtta tttatatgtg
    10621 caactgttgc aagggacaca aaaacatcta tattagctat agtcttagcc actttttgta
    10681 ttctatctat gtttttatat attgtatctc ttatctctac aaatatctcg tattctaaag
    10741 atttaatctt ttcctctgca tgtagtatct tttcttctat ttcctttaat tctggagtta
    10801 tatatctttc tgcattacta agtgtttgct ttcttatata tgtctcatct agctttgcct
    10861 gtttaaagtt agcttttgtt atttctatat aatatccaaa aactttatta aatcctattt
    10921 ttaatgattt aactcctgtt ttttctcttt ccctgttttc tatttccttt acaagaaagg
    10981 ctccattctt agaaatctct cttagttcct taagttcatc actaaaatct gatttaatta
    11041 tgtttccatc ttttatggtt atcgtaggtt cttctagaat agcttcatct attaaattgt
    11101 atatatcatc taatttatcc atttctgaaa catactcttt taggattttt gcatttgata
    11161 aatttatagt atcttttaaa ttaggtaact tttctatcga attttttaag tgaattaact
    11221 cttttggtgt tactctctca aatgctatct ttccacatat tctttctata tcgtatatat
    11281 ttttaagtat atcatttagg tcttctctta atataaagtc atcttttatt tcttctatta
    11341 catctaatct attttcaatt ttagatttat ttatcaaagg ttcttctaca tattttctta
    11401 aaagtctacc tcccattgct gtagaagttt tatcaagtac gtgtaataat gaaccttttt
    11461 tcttattccc tcttatagtt tgagtaagtt ctaaattagt tctagtaaac atatctagaa
    11521 ccatatattc tgatgaattg tatatattta tattattaat gttagatgtt atttgttttt
    11581 gagtattgta tatataattc agaagaattg ataagcttga ctttattaga cctttatcat
    11641 caaactttag tttttgtaga taaacatctg gaaaatattc ttttaatata tttatatcca
    11701 aatagttatc actaaaactt tcatttatgt atatattact tacagtagct atatctctaa
    11761 gcttttctat aaagtctaaa tcatttataa ttatttcagt tggatggatt tttgcaattt
    11821 cttcaattac cttatcttca tttaaacatg ttgcattagt ttctcctgta cttatatcaa
    11881 cataagttag acctatatta gtaccatcct tatacaaaga taataaataa ttattctttt
    11941 tattttcaag taaatttcca tctaaaactg taccaggagt gatgaccctt ataacctctc
    12001 ttctaacaat tccttttgct gtagatgggt cttccatttg ttcacctatt gctactttat
    12061 atccattttc aactaatttt gatatatatg aattggctga gtggaaagga acaccacaca
    12121 taggagcacg ttcttctaaa ccacaagctt ttcctgtcaa tgctatttca agagcttttg
    12181 aagctactaa tgcatcttca aaaaacattt cataaaaatc tccaagtcta aaaaacagta
    12241 tacagtcttt atatctattt tttacttcaa gatactgttt catcatagga gttaatttat
    12301 tcatatctat ttgcattatc aaacctcctt cttttctaat taaatattat atcatagtag
    12361 atgattatta ttaacacaat atataaaatt ttgacatata actaaaaaca atccaaaatg
    12421 tatactcaca ttttggattg ctaagtttaa tgcttattaa ataaaaatat attttttttt
    12481 acaataaaga tattatttct tttttaagtt taatgatatt ttcaaggtta tcttcattta
    12541 actttctact atctataaca aattctttct ttactattga tggttttttt gaaataacat
    12601 aaattctgtt tgatagcatt atagcttcgt caatatcatg agttataaga agtattgttg
    12661 aatttacttt tttcttaaca tctaaaagcc atttttgcat ggatgtttta gttaatgaat
    12721 caagtgcccc aaaaggctca tctaaaagca ttatgtcatt agaatttata aaagttctca
    12781 agaagttagc cctctgtctc attcctccag aaagttctga aggatattta tcttcatatc
    12841 cgcttaatcc aaacacctca aaataaggtt ttaccattga cctagcttta ctcttttttt
    12901 ctttttttaa aattagtggt agtgacacat tatctattat agttttatat ggcaagagta
    12961 aatctttttg atacatataa ctatacttac catttatatt cactttgcca ctatcataat
    13021 ctgtaagttt agttataata ttaaaaatag tactttttcc acatccactt ggccctaata
    13081 tagaaacaaa ctcaccttca taaacatcaa tacttatatc ttttaaaact tcaatatctt
    13141 tataagtttt tgatacattt tctatagata cttttacttt tttattgtgg taaaaattca
    13201 tttgtgtaag catcacttgc cttcatatcc ttatctatta atttatattc ttttaagaat
    13261 gatgtatagt tgttccatac actatctttc atttctcccc atcgtggtgc atcatctttg
    13321 tacttgcttg ctaaatattc ttgactcttt aaagctagtt tttcatctac ttcaggtgca
    13381 tgttttacta aaatttttgc tgattcttct ggatttttta ttgcatattc ataacctttt
    13441 gttgtagctt ttaagaattt tttagcaagc tctggattat ctttaagcac agtttcatta
    13501 gaaattataa gtggtgtata ataatccaat ctctcatcta aatcttttac tggtataaag
    13561 tttaagtcat atccaattaa atcagcttta actgcatccc aaccttcaaa tatccatgca
    13621 aaatctacag ttttcatagc tgcaaagaaa tcatcttgac cagtattaac tatctttaac
    13681 ttattaaaat cagctccatt tttcttcata actgctttaa aaacagcttc ttctgatgga
    13741 gagccccaac caccataagt ttttccttca aaatctttag ctgttgttat attcttttcc
    13801 tttggtgatg caaacccaga agtattatgt tgtataacag ttgctacagc ttttattgga
    13861 agtggatctt cactagtctt tgcataagta acttgttctt gatagcttat accaaagtca
    13921 gcttttcctg ttgctactaa agtttctgct ccactttcag gtggttgtac tatttcaaca
    13981 tccaaacctt cttctttata gtaaccctta tcaagtgcta caaataatcc agtatgattt
    14041 gtatttggtg tccaatcaag caccatagtt acttttttag aagaagtatc tttatcattg
    14101 tttttgtctt tattgttatt tgaagaacat ccagttattg ttgccaatgt aaaaacactt
    14161 actaaaccta atattcctaa ctttttaaaa ttcataattg cctccctcaa atacctttaa
    14221 tctattaatc tatgtcttat aacaactttt tttacagcct gtacaattcc tacaaacatt
    14281 aaactaaata taactactaa tatagtagat gcaaatactt tatcaagtgc atatgcactt
    14341 tttgacctaa gcatataaac accaagtcct ttcgttccac ctagccattc tgcaacagtt
    14401 gctgccatta cagcatacgt tgcagatatt ttaagtccag aaaaaaattt atccattgcc
    14461 ataggaaatt taaggtgaat gaatgtatta actttatttg agtccatagt cttaaataaa
    14521 tttaaataat ctttatcaat attctcaatg ccatctacaa aacttattaa tataggaaaa
    14581 aaacaagtaa gcattaccat caatactttt ggcattgttc caaatccaaa ccatatcata
    14641 aagagtggcg ctataattat tgtaggtatc atctgtgtaa ccaacataat aggataaaga
    14701 cattttttta ttattgatac aaaatccatc aatattccaa ttaccaatga tattataatt
    14761 gcgactataa aaccaagtat tgcttctcct atagtaacca cagcatgcat ggataaattt
    14821 tgataatctt taaaaaacac attaattata tctactggtg tgggtagtat atactgagga
    14881 acttctaaaa ctcctaccat agattgccaa agaataagta taaacaaaaa tgttgctatt
    14941 ggatatatac tatctttgac tttatctatg atttctaagt ttttcatcta tagtcagacc
    15001 tccagctttt tcatctactc tagctactgt tatgactctt tcacatccaa gattaaaagg
    15061 gattttatgt acctttttta ataattcaaa aacttcatca aaatcacctt caacagtagt
    15121 gctcattgct cctatttcac atttaagacc acttttttca atttcagcaa tacaagcatc
    15181 tactacttta tacatctctt cttcaccttt gtaaggtctt agtggcatta ctgctatgtc
    15241 tgcgattacc atttaaatca ctcctttaaa aataattaat acctactagg tatattttgt
    15301 tttttattaa aaaattttat tactaaaaag atattatatt taaataaaag aaaagtctta
    15361 tccaaaatag gataagactt atttttatct taactggtct ccctacactg gcattatcca
    15421 aacaggttca aagggtcgag ataaaatctc ctctcagcac attagctccc cgaaatatat
    15481 tatatctttt ctacttgatt atactacact tatcatattt taacaaattt tttatttata
    15541 ttagatattt tatccgttca aaaatatcta atataaattg tacattatta caccaaataa
    15601 attatcttaa aatggtaatt tatcaacatc ttccaaaact agattaactg aattttctag
    15661 tttttatttt gctatttcaa gcatatttgg atgtatatca attccataca aatattgtgc
    15721 taaatttctg ttttcactca tctaatttgc aatcgttaca gattacagat tagaaacagt
    15781 ttttagactc tacttatctt tatctacaaa aaatccttca ataaattcag taataatatt
    15841 cagtaatcta aaaataaacc tctatctaga tttttaagat acaagccttt taataagtac
    15901 cttttgtgat aatataaaaa ttactcgtca ttatcatcaa aatcctcagc aaccaacatt
    15961 ttagataaga atcaaactct ataatataat tattttatcc aatttatttt tgagaaataa
    16021 aatatttttt taatttttaa attttttaat atgcaccttg aaaaaagaaa cgttttccta
    16081 tattatggta atagtagaat aaaactaaat aggcaaaact agagaaattt agtgacgcaa
    16141 agctataggg actaagactt atgaaatgag ttatgtcagc cagttgccaa agagatattt
    16201 tctttttgtt tttatgaatg ttttttaggg ggatatatga tatatgttta tggataaaat
    16261 ggatagatgt acacatatac taactgcata tatatgcagt tcacgcgatt actgcaattt
    16321 tatagataca caattaaatg attttatact agaatatgga gagaatgtgg tagaatcttg
    16381 tttgcatcaa gtgatggtat tggtaagcag atataattaa agaaatttta t
//
LOCUS       DAEMHZ010000090        16382 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000090 DAEMHZ010000000
VERSION     DAEMHZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.090"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..924
                     /locus_tag="KPK72_002597"
     CDS             145..924
                     /locus_tag="KPK72_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2489798.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1234..2055)
                     /locus_tag="KPK72_002598"
     CDS             complement(1234..2055)
                     /locus_tag="KPK72_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG2489799.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYSDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(3064..3882)
                     /locus_tag="KPK72_002599"
     CDS             complement(3064..3882)
                     /locus_tag="KPK72_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2489800.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHTLKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4050..4631
                     /locus_tag="KPK72_002600"
     CDS             4050..4631
                     /locus_tag="KPK72_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2489801.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4628..4822
                     /locus_tag="KPK72_002601"
     CDS             4628..4822
                     /locus_tag="KPK72_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489802.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4986..6728)
                     /locus_tag="KPK72_002602"
     CDS             complement(4986..6728)
                     /locus_tag="KPK72_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG2489803.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGDIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            7157..8533
                     /locus_tag="KPK72_002603"
     CDS             7157..8533
                     /locus_tag="KPK72_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG2489804.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8820..9491
                     /locus_tag="KPK72_002604"
     CDS             8820..9491
                     /locus_tag="KPK72_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG2489805.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9682..10479
                     /locus_tag="KPK72_002605"
     CDS             9682..10479
                     /locus_tag="KPK72_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG2489806.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANFDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    10823..11057
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11107..12126
                     /gene="pheS"
                     /locus_tag="KPK72_002606"
     CDS             11107..12126
                     /gene="pheS"
                     /locus_tag="KPK72_002606"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG2489807.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            12143..14536
                     /locus_tag="KPK72_002607"
     CDS             12143..14536
                     /locus_tag="KPK72_002607"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG2489808.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAINGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGVNSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            14561..15151
                     /gene="zapA"
                     /locus_tag="KPK72_002608"
     CDS             14561..15151
                     /gene="zapA"
                     /locus_tag="KPK72_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG2489809.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            15405..16088
                     /locus_tag="KPK72_002609"
     CDS             15405..16088
                     /locus_tag="KPK72_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG2489810.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         6212 a         1895 c         2718 g         5557 t
ORIGIN      
        1 tttatgctat ataacaatta ttgaaaatga tataaatata atatctcaaa taatatgaat
       61 ataacatctg aatagagggt gttttatttg taaaaaataa gtttacagtt tgagcgtata
      121 aacttatggt aaaggagaat aattatgagt tataagaaga ttgcaattat aacgggtgct
      181 aatagtggtt ttggtaaaga gtttctaaag ttattaataa atgaagaaga aataacagaa
      241 atatgggcag ttgcaagaaa caaggcgaga ttaaatcaat tagtagatga atttggaagt
      301 aaggtaaaaa ttttttcaaa agatttatca aaaatagaag aagtaaaaga aattggtaca
      361 tttttaagta aagaaaatgt atgtataaaa taccttataa acaatgctgg ttttgcaaaa
      421 ttctgttcat acaatgattt aagtatagat gaatctatta atatgattga tttaaatatt
      481 agtgctgttg tagcattggg acttatatgt attccatata tgcaaaaggg aagccatatc
      541 atgaatattt cttcacaagc atcattccag ccattgccat atcaaaatat ttatagttca
      601 actaaagcgt ttataagaaa ttatacaaga gcattaaatg ttgaacttaa gagtaaaggg
      661 ataaatgcaa ttgctgtatg tccaggttgg atgagtacaa atctatttaa gagaggtata
      721 gttgtagctg aaaaagggac aaaaaatttc ccaggcatag tgtctccaga cattgttgcc
      781 aaaaaagcat tgaaggatgc aaagaaaaat aaggatattt ctgtgtatgg tataaataca
      841 aaatccagtc atttattagc aaaactattg ccacaaaaaa tgatgatgaa ggtttggtta
      901 atgcaacaac atattaagga ataagagttt cggtaattaa gtatcagttg gagattcaat
      961 ctgttatata ttatgagaag ttttgttgag caaataataa ttgtgacaaa acggcttatc
     1021 tagtgccgat aagccgtcta taatgtatat aaaatatatt tttgatttta ctttatttag
     1081 atattgaagt attaaaatat gtaggacttg aaattttata aagataaata ctatgatatt
     1141 ttgtaaaaac tgagatatgt tgatatcaca tagtttgact atactgttaa attgaagttt
     1201 tcttattttg cattatgctt attacttaat gatttagact ataatttcca taccatcata
     1261 tgcaaaataa atgttatcaa gtttcttttc taattcactg taataaccat atgattttcc
     1321 ccacatttcg tcaatatgtg taacgataat ctttttaatt ctataatagt ttttaatttc
     1381 aacaatctca tctaaagtaa atatttcatc tttaaatggt gtgtctttaa gttttgtacc
     1441 atcatttaat ataccatcat cagaaactag acttattatt aaaatatcag catcaaagta
     1501 catatcacta tgagtaaatg gcttgggatt acagcaggca tatatgactt ttttttcatt
     1561 ctctttgata acatagaagg ttatattcct gtctgggtta ttgttaatca aatcaatatc
     1621 aaaattattt atctttacat gcgaagtttc attaatagaa atgcaattta taacatcact
     1681 ataatattta agacaatcga gattcgttcg gtttatatct tcaattactt caggaagcgc
     1741 ataaaagcca attggattat tttctcctgt gataaagtca catcctattt tttcgacaat
     1801 tctaatgccc ttaacatggt ctgggtcatg gtgagaaatt gaaatatgtt ttacttccga
     1861 tattttttgt ctgttacaag ctactgcaat gtcttcaggt gtgtctatca gcatttttat
     1921 atcatcaata tataaacttg gccctaatcg ttcatatctt ccgccttttt gtctggcttc
     1981 ttcacatatt ttgcatttgc aaaatgaact tggaataatt gacataccgc cactaccaac
     2041 aatttttaat ttcaaagtta ttacaccacc ttcttaaaat attagattat ataaataata
     2101 tacaagcaca actgattaac aaaattatgg gttaataaat tatatcacat gtaaatatga
     2161 aagaatagta tgaaattttt ataagcatat attaaatatt tctcaatgaa attgactttg
     2221 tatatgaacc gaatgactta ttcaatatgg ataagacatt tttcttgtaa taagatatga
     2281 ttgtcctata aggtttatgt aattttgata taatttgata taatatattg attaacaaca
     2341 tacatgttat tggtgagcat atttctatag aatggaatat ataatttttt atatttatca
     2401 ttatatcatt gtctaaagaa agttaaaaag agtagagcac aaaggaatta tttgtacttt
     2461 actcttttat tgttttgtta aaaaaactac tttatattag tgattaaaat atatttaact
     2521 agagtataag gatagtatat aatcttataa ttgatttatc tgacaaatta agataatatc
     2581 ctaatcaaat tctagtcatt taaacataaa atcataattg atttatctga caaattgaga
     2641 taatatccta attaaattgt aggtatttaa acataacatt ataaataatt tatctgacaa
     2701 attaagataa tatcctaatc aaattctagt catttaaaca caacatcaca agtgatttat
     2761 ctgacaaatt aagataatat cctaattaaa ttgtaggtat ttaaacataa catcacaagt
     2821 gatttatctg tcaaattaag ataatatcct aattaaattc tagtcattca aacataacat
     2881 cacaagtgat ttatctgaca aattaagata atatcctaat taaattgtag atatttatac
     2941 ataacactaa aagtaatttg tttaacaaat taagataata tcctaattaa attgtaggta
     3001 tttatacata acactaaaag taatttgtct aacaaattaa aatattatcc aattaatata
     3061 aaattacttc tttttaactg ggaagcatac ctctgttacc caattatcag gattactatc
     3121 gttgccagga cttacatgat aaatattaaa cataggacca tccacatcat agttattatc
     3181 tgatatccaa ttaccaatag cctcacaagc gtctgcaact tgattgaaat ttccattaac
     3241 aattgcagtt gcagcagtca cacttggaac ggtcttaaat ttaacatgtt cagtatcttt
     3301 atactttcca gatacagcaa cttgtacttc aacatctaca taatcttctt tatatccagt
     3361 atcataaaag actgctttag aatactttgg gggttcaatt tgaacattct ggtcttttgt
     3421 ctctaaaaat gcttgatgcc ataacattcc ttcgtcttga taagttggta taacccttct
     3481 aagtgtcatc atatattttg gtgcaaaatt ttttatagtt acatcataat ttttcataat
     3541 gtcatttcct ccaattcttt taatagtggt ttctattttt aatagttttt tctgtgtatc
     3601 ttctaattgt tcttttactt gagaatggtg gatatttaga tattttatga gactttcttt
     3661 atcgttatat tctgtcaaaa tttcttttat agaatacaag ccaaagccca tatcttttag
     3721 tgtatggatt ctgtttgtag tagaaagttg gtctgcacta tagtatctat atccattagt
     3781 tttattcgta tgagaaggag ttaaaagtcc tatttcatca taatgacgta acatacgaat
     3841 actaatttta cttagctttg aaaaatcgcc tatcttaaac atgtttttgt ctcctttata
     3901 aaggtacttt tagcttaata ttaatataaa ccatatcata atgttagagt caactaaaat
     3961 tataattaaa ttatattgac acatgatgaa aaaaatatta tactgaacat gttcagaacc
     4021 aaaaatgtta attattttgg aggtctatta tgaaaagtaa tgataaagat gctagagaac
     4081 gtataattga agttacattg aatcttttaa atgaagtaga tgatattgag gagattacag
     4141 ttagaaaaat tgctgagcgt gcaaatgttg gagttggttt aataaattat cattttaaaa
     4201 caaaggataa tttgttgagt actgccatag gagatgtgat gtcaaatata atagcagagc
     4261 tatatgacga tagtgtatat actctaagac ctattgaaga tttgaaaaat ttattaaaaa
     4321 aattgtgtga tactggatta cattatgaaa aagtgcttcc ttttgtgttg aatcaatgta
     4381 taaccaatgg agatatgcaa gcagaacttg atattgtccc tatgcttcgt aaaatttttg
     4441 gaaataaaaa agatgaaatg agtctaagaa tcattgcgct gcaaattatt ttaccaatac
     4501 agatttctgc tttatctaca gaatcatttc agttatacag tggtataaat ataaagaata
     4561 aatatgagcg agataaattc attgatattt taatagaaaa tattattgga gaggatgttg
     4621 atgtaagatg aaaattataa ctattggttc aagtcttatc actgttttac tttttctttc
     4681 aactatggtt tgtgggttct ggattaagaa taataaggta acagatgcaa gttctattaa
     4741 gttccatatg aatagtgcta tttttacagg gatattttta ttgatttcca caatcctttt
     4801 aattatatat ataaaaaaat aatattatta ttaataaata aagtatcaga aattataaag
     4861 acaagagtct taaaaatttc tgatacttta ccatataatt ttattaaaca ttaattgtgt
     4921 gtgaaaaaat atcacttatt tcaatctaat actaaatctt aagagtgagt ataaagtatt
     4981 agaaattaag aagcattttt tagttcaagt ggattatcta taccaaaata atcatataaa
     5041 gtagagctta tttcctctaa atttccatct tggaaaggta attgtaaatt taccttttct
     5101 accacatctt taaatccagc atcagtattt tgatttaaaa aatccatata tacatcaaca
     5161 gcttttctgt aatcttcttg tgataattgc catatctgaa gtgatacatc agatgcaaga
     5221 ccataactca agtaataaaa aggaacttgg aagttatgag atacaagtat ccaaaaagga
     5281 gcattttttt ctctaagtat attattggaa actccatatt cttcctctaa atcaaaaaac
     5341 aatttattga tatcatttag actcatatat ggattcttgt atataatttc ttgaaattca
     5401 tcataaagac aagcatcaat aatagtattt aaaacaatcg ataagtgttc tttttttata
     5461 gcttcagatt gtttaccaaa aaaatcatca aaatatttat aaaatagaat ttctaaagat
     5521 gttgaatgaa cttcacatac atctatactt ggttgcatct tattatttaa gttaatactg
     5581 ttgtagtaat tataaaaatg accaaactca tgagcaaaac tagtaacacc taaaaaagaa
     5641 ttgtcccatg tattaaacaa gaaaggtgct ttatatttat ttatataagt agtatatcct
     5701 ccaggcgatt tgttttttcc agaagaataa tcgtacaagt catatttttt catatagtcg
     5761 aagctctctt ttagttttgg agatatatct tcaagtactt tatcaaactt cctaaatgag
     5821 cgatttttat atactttaat ataaatagaa gaatcagatg gtttagatga aatctctcta
     5881 tataatggaa ctatgtaatc ttttacatct tgtctaaatt ttttagcttc ttcttgagaa
     5941 tagtctttgt ttaggttcat ataggcataa tctatataat tcttaaatcc ttgcttactt
     6001 gctatttcag ttcttgtctg tataagttct aagaatatgt caccaaatat aggattgtat
     6061 ttttttaagt aatctgaata aattttcaca tattcttcag ggctaagatt tggtcttgac
     6121 aaggcttctt caaagtctac ctctatacca tctattgata ctgtagattt agctaataag
     6181 tcttcatatt ttacaactag gtcttgctct tttttaagca aaccttcaac ttctttagaa
     6241 ttaagttttc caatgttctt tagataatta aatgtatcct ttccaagttc tttttctaat
     6301 tctttagaga atttagaagt tacaaatcta tcttgaaagt ctttgtatga catgtcaaca
     6361 tctactgatt tactcataag atacttatat tccttagagt aaaattcgtc tgatgaatct
     6421 atgttatttc taatttttgc tatagtaagt gtactgtaaa aatcctgata aattttatca
     6481 acttcattaa ataaatttaa ttgttcctta gctgttttag ctgttaaaag tttgtcgtta
     6541 cacttgttaa tgttttcgca aattgactgt atattaggac gcttatattc cattttttca
     6601 aaaggaatag ttcctctaac agaatctttt tttgtatcat tagtataatt tcttatgtat
     6661 gatgcaaata taataaaaac tgcaattaaa attagaaaaa caaatatttt cttcttggat
     6721 ttaaccataa aaatcctcca atcatggata tagttattaa ttattttact atattaaata
     6781 taaaaaatgc atgttaagaa aatttaacat aaagatatag ataagattat aaataatata
     6841 aaaaatttaa cttcaaaaaa gtatatgaaa tttatactgg taaaatgata aataaatttt
     6901 aagtacaaaa tagtaaaagc tatgatagtt ttagacccat tatatgtaaa aaatatagac
     6961 gtttttggtg caatttaaat taaaagatat atataaaaat ttaaaatatt ccaaacagaa
     7021 attataaaaa ttactatcat tgtctttatg caatatttat actataatag aataataatt
     7081 tagaggaaag ctctaaaaat aaacaaatta agggtcttaa cagtagtgat tttatgttaa
     7141 ggaggtataa agaaagttga aaacaatagt tagaagactt attctctacg cagatggaat
     7201 gcgtcctact caaataatgg tatctgggtt tgctgcgatt attgtaatcg gagcattgtt
     7261 gttaacactc ccgatagcgt cgcagagtgg agagagtata ggcttattaa atgctctatt
     7321 tacggctact tcagcggttt gtgttactgg tctagttatg gtagatacag caacttattg
     7381 gagtttattt ggacagatag ttataataac attgatacaa attggtggtc ttggatttat
     7441 gaccgtagcc acaatgtttt cacttatggc acgaaaaaaa atacaactaa gagaaagatt
     7501 attaatacaa gagtcattaa atcaggctga tttatcagga cttgtaagac ttacaagatt
     7561 tgtattaata ataaccataa caatagaggg aataggtgca ctggttttat caactgtctt
     7621 tattccacaa tttggtttgt ctaaagggat atggtatagt gtatttcacg caatatcagc
     7681 gttttgtaat gccggatttg acttgatggg cagtgtaagt ggcccattta catcgttaaa
     7741 ttcttatgta aacaacttta cagtatcaat gactgtttgc gcacttatag tgcttggagg
     7801 gctaggattc cctgttgtac ttgatattgt aagaaaaaga agattttcaa aattaaatgt
     7861 gcattctaag gttgtattgt tttcaactgc tacacttatt tttgtgggtg cattatttat
     7921 ttttcttata gaatttaatc aaaaagctac tatggcggac ttgcctttaa agggaaaagt
     7981 actatcagca atatttcaat cagtaacagc aagaactgct ggttttaata cgcttgattt
     8041 agctacttta cgagaaagta gtgtatttgt aatgataatt ttgatgttta ttggagcatc
     8101 acctgcatca actggtgggg gtataaaaac aacaacacta gctgttttaa tcattacagt
     8161 tagaagtttt ctatcaggaa aatcagatat agaagctttt gaaagaagat tagctccatc
     8221 aactataaaa aaatctcttg gtatatttgt aatcagtata tcagcagtta tttttggaac
     8281 attgattatt tcaataactc agcctaattt tacattagtt caatcagctt ttgaggttac
     8341 atcagcactt gctacagttg gttcaagttt agctggaact ccaaacttaa atgctttagg
     8401 taagattata ataataatct ttatgtttat gggtagagtt ggttctctta ctttatttat
     8461 ggctatactt tctggcggta gaagaaagag tcaaccaatc agatatgcag aaggtaaaat
     8521 tatggttggt taagttacca aggagtagta taaatttata atggatttag agtagcactg
     8581 taaatctgtt gataaaataa atgagtctag atgtaattat atgaagaaat aaaatcgact
     8641 aatgaacaaa attaattaat gaaataaaaa aacagggaaa taaaatacaa ataaaaaggt
     8701 aaatcaaaat taaattaatc tgtgtatata aaattatata ttgtgtataa gttttgtatg
     8761 tgtaaacaat taatttaaga gatattatat aaattgtgta ttagggagga agtttcgaga
     8821 tgaagcaata tatagtcatt gggtgtggga gatttggaag ttcagttgcg tctactatgc
     8881 atcttttagg acatcaagta atggcaatag acaaaaatga agattcagtt caaagtatat
     8941 ctgacaaggt aacccattca cttatagtgg atgttactga tgagcaagcg ttaaggtcat
     9001 taggtttagg taactttgat gtagcagtag ttgcaatagg ttctgatata agggcatcta
     9061 taatggcgac tcttatagcc aaagaaatgg gtgtagagtt gataatatgt aaggcaaagg
     9121 atgaattaca agctaaagtt ctttataaaa ttggcgcaga tagagttgta tttccagaaa
     9181 gagatatggg agtaagagtt gcacacaatt tagtttcgga taatatatta gaccatattg
     9241 aacttgaccc agagtattca attgttgaaa tcgtaactcc aaatagttgg gttggcaaga
     9301 cacttataga gcttgaatta agagctagat atgagataac tgtacttgct ataaaaacag
     9361 gtaaaaatat aaatgttaca ccttctccag atgaggagct tacagccgga agtatcctag
     9421 ttataatcgg tcaaaatact agtataacag cgataacatc tggaaataag gggataatta
     9481 gaagaagata atttactatt taatatatat ttaattgcaa tgaaagtaaa gagtatcata
     9541 taattatgaa tagttatatg atactatttt tatttaatcg aaggtagtat ttatttgtaa
     9601 gattagataa agagaagtta aattaaaagt aaggaggctg tgctaatata aaaatttata
     9661 agttattagc atttagataa aatgattaca aatataaaca gtaaggataa tgaaaagtta
     9721 aagtatacaa gagcactatt aaaatcaaag aataggaata aagagtcaaa gttcataata
     9781 gaaggataca gaatagtaat gcttgcactt gaatgtatgg caaactttga ttatgtattt
     9841 atcaatgaag aatttgaaaa taagaaagaa catgtaaaac tattagaaga tttggataaa
     9901 aaaaacacaa agatatacaa gactactaat aaaaacttta aagaattagt ggatacagaa
     9961 aatactcaag gaataatagg tgtagtttca tttaagaaaa aaaaattaag tgaaagtata
    10021 aataaaaaag ataaatttgt attgatttta gatagaatac aagacccagg aaatatgggg
    10081 actataataa ggactgctga ttctgctgga gtagatgcca taatagcact aaagggatgt
    10141 gtcgatatat acaacccaaa agtaattagg tctactatgg gttctatttt tgatatgaat
    10201 ataattgatg cttcacaaga tgaaactgtg gatatgctta aatcattgga ttttaatata
    10261 gtttcaagtt acttaaatac agaaaatttt tatgacaaaa tagattatgg ttcaaaagta
    10321 gcattggtga taggaaacga agcaaatgga ataaatgaag aacttgtatc aaagtctgat
    10381 attttggtta agatacctat atatggtaaa gccgagtcgt taaatgctgc gataagctct
    10441 gctatactga tgtatgaaat aaaaaaatac ttaatttaat gtattgtaat aaatataatg
    10501 tcatgttata atcttgaatt aaatagtaga gtatcaaaaa tagtcaatat atattaaaaa
    10561 aataattaaa ttaattatat attagatgta tatcatataa aaaataattg agttgattat
    10621 gtattagata tatattatat aaaaaataat tgagttaatt atgcattaga tgtatattat
    10681 ataaaaaata attgagttaa ttatgcatta gatatatatt atataaaaaa taattaaatt
    10741 aattatgtat tggatatata ttaaaaatag tcacataatt tgaatggaaa tgatataata
    10801 ctaaaataaa caatataata ttgtaaatgc aatgaaagag gaaagtattt tgattaaact
    10861 tagtaaagag ataaacacct aggctgggag tgttttctaa gaggtcatga gaagttccct
    10921 ctggagtaac agaactgaaa ttttacagta ggctttgacg tcaaaaacgc gttaagtttg
    10981 ttagaggtgg tttgatgatt tttaaattgt taaactacta gggtggtacc gcgaaactat
    11041 agcctttcgt ccctagacag ggattgaggg gcttttttta tacaaaaaaa cgaaagggtg
    11101 atatgtgtgc aagaaaaatt acttgcttta cgtgaagcag ctttggctga aataaaagaa
    11161 gcacaaagca tagaaagtgt agaaagttta agagttaagt acttaggaaa aaaaggtgag
    11221 ataactgcca tacttaaaga aatgggtaaa ttatctgctg aagaaagacc agtagttggt
    11281 aaggttgcca atgaggtaag agaaaacatt gaacttagca taaattctaa aaaagaagaa
    11341 ataaatgcta ttgaaaaaga aagaaaatta aaagaggaag tgatagatgt tactcaacca
    11401 ggaaaagttt taaaggttgg gaagaagcat ccaataactc aaattataga tgaagtaaca
    11461 gatatattta tcggaatggg attctctata gcagaagggc cagaagttga gactgttgaa
    11521 aacaactttg acgcattaaa cgctcctaaa gaccatccat caagagatat gagcgataca
    11581 ttctatatca atgatggggt attacttaga actcaaacat ctccagttca agtaagaact
    11641 atgagaagtc aagagttacc aataaaagta attgcgccag gtagatgctt tagatcagat
    11701 tcaccagatg caacacattc accaatgttc catcagatag aagggcttgt tgttggaaaa
    11761 gatgttacta tggcagaatt taaaggaact atggatatct tcgttgaaaa attgtttggt
    11821 tctgatatca aaactaagtt tagacctcac aacttcccat ttacagaacc aagtgcagag
    11881 gttgatgtta cttgtttcaa atgtggtggt aaaggttgcc caatgtgtaa atatgaaggt
    11941 tggatagaaa tattaggttc aggtatggtt catccaaatg tgcttagaaa ttgtggaata
    12001 gacccagaag tttacagtgg atttgcattt ggagttgggg ttgaaagact tgcaatgctt
    12061 aaatacgaaa tagatgatat tagattatta ttcgaaaatg atatgagatt cttaaatcaa
    12121 ttttaattag gagggtatgt agatgttagt atctttaaaa tggcttagag actatgttga
    12181 tatagacatg gatgtaaaag agttcgctga caaaatgaca atgacaggaa ctaaagttga
    12241 aacaatagat tattatggtg aagaaataga aaatatattg gttggaaaga ttttagaaat
    12301 aaaacaacat ccaaatgctg ataagttggt tgtaactaaa gtagatattg gagataaagt
    12361 tgttcaaata gttacaggag ctacaaatat atcagaagga gattatattc cagtagctat
    12421 aaatggttct aagttacctg gaggagttga aatcaaacag actgatttca gaggtgaatt
    12481 atcagatggt atgatgtgtt cagcagctga actaggtata gatgaacatt acattgagga
    12541 gtataaaaga ggtggtatat atattttaga ccacgaagat tcttatgaat taggaaaaga
    12601 tataaaagat gttttaggat taaaagatgc tttaatagat tttgaattaa cttcaaacag
    12661 acctgattgt aaatgcatga tgggtatagc tagagaagca gctgcaacta taggaacaaa
    12721 agtaaaatat cctgaaatcg aagtaaaaga aagtgacgaa gagatagatt tcaaagttga
    12781 gatagataat ccagatttat gtagaagata tgttgctaga atggttacag atgtaaaaat
    12841 agaaccttct ccatattgga tgcaaagaag acttacagaa gcaggagtaa gacctataag
    12901 taacatagtc gatataacaa acttcgtaat gttagagctt ggtcaaccac ttcatgcttt
    12961 tgatataaat caagtagaga ctggaagaat agtagtaaga aatgctaaag atggagagaa
    13021 acttgtaaca ttagatgatg ttgagagaac attagataaa gatatgctag ttataacaaa
    13081 tggagaaaaa tcacttggtt tagctggtgt aatgggtggt gttaactcag aaataacttc
    13141 taatacgaag actgtacttt ttgaaagtgc caatttcaaa ccagaaaaca taagaatgac
    13201 agctaaaaaa gttggtatta ggtcagaagc atcttcaaga aatgaaaaag acttagaccc
    13261 taatcttgca gagatagcag caaatagagc tgcacaactt gttgaaatgt taggagcagg
    13321 aaaagtttta aaaggtgttg tagatgtata tccaaataaa ccagaaccta aaaaattggt
    13381 agtaaatcct caaagaatta accacctatt aggtgtagat gtaccaatgg agcagtttgt
    13441 aggaatttta gaatcattag agtttaaatg taatttggta gctaatgata aattagaaat
    13501 agatgtacca agctttagaa cagatatgga acaagaagct gatgtatggg aagaaatagc
    13561 tagaatttat ggatttgaga atataccttc tgtacaatta gaaggaaata cgacagcagg
    13621 aattaaaact tcaaaacaaa aattcatgga tgctttaaaa gataattcaa ctgctgtagg
    13681 attaaatgaa atattaacat attcatttgt aagccctaag ggagtcgata agataagagt
    13741 accagaaggt aatgctaaga gaaactttgt taaattactt aacccattag gagaagaaac
    13801 ttctgtaatg agaactactt taataccaaa tatgttagat gttttatcaa ctaatgtatc
    13861 tcataaaata gaagaagtat ctgcatttga gtgtggacat atattcatcc cacaagattc
    13921 agaattacct aaagaagaaa atagaatgtg tgtaggtatg tatggtaaag atgttgactt
    13981 ctttgcacta aaaggaacta tagaaactat acttgtaaat gtcggattta aaggttatga
    14041 aattgaacct caagataata atactacctt ccaccctggt agatgtgcta aaatagtgta
    14101 taataataaa tatgtaggta cattaggtga attgcatcca gatgttatag aaaactacaa
    14161 tctaggtcaa agagtttatg tcgctgaaat agatattgac tttgtatttg ataattctga
    14221 tagaacaaag aattatgtgc cattaccaaa atatccatct acatcaagag atatagctct
    14281 tattgttaaa gatgatgtat ttgtaaaaca aatagaagat ataataaagg aaaatggtca
    14341 aggattagta gaaagctaca agttatttga tgtttacaaa ggttctcaaa tagaagctgg
    14401 atacaagtca attgcgtatt caataactta tagaagtaaa gataaaactc taacagatga
    14461 agatgtagct aaggtgcatg ataagatatt aagtgaatta agcgaaaaat taaatgctaa
    14521 cttaagatca aattaacaac taatgtcggg ggaggttagg atgaacaaag taatggttaa
    14581 aatccatggc gctgaatatc caatggttgg agacaaatca gagaagttta tgattagtat
    14641 tgctgatttt gtagacaaag aaatggataa aataacacgt caaaacccga aattgagctt
    14701 atcagttgct gcgatactaa ctgcattaaa tatatcagac cttttatttg aatgttcaga
    14761 tgagaatgag aagttgatta aagctaatga agaattgagt aagaaggttg gagcatctaa
    14821 tgaagagctt caattagaaa tcaagtcttt aaagcttacg atagcagaaa aagaagcaga
    14881 aaatcgtgaa gctgagacta agatgaaaga gttaattgag attatagaaa ataaaaagca
    14941 agaaatattc gaacttagta atacgacaga aggttctaga gcagagttag atgcttataa
    15001 aaacaagatt gaggagttaa gtactcaact tgaagaagca aatgaaagag ctactatagc
    15061 tgaaaactta gcttctgagt ttcaaaacaa agcttatgat ttacagttaa aatgtacagg
    15121 gttaaataat gatgcaaaaa atgtggaata aactaaaaga tttgctgtct cttgtttttt
    15181 gagacagctt cttttaatgt ataaatataa atgctatata aatatagata atgtttaaat
    15241 gtaaataaat ataaatgcta tatagatata gataatgtat aaatataaat gatgtataaa
    15301 tatagacaat atttaaatat aaataatttt tttatatcaa tgaaaaatag aatctaaaat
    15361 gattttgatt ttgtatctat cattagaaag cgagaggttt atatatgaca agcaagagtc
    15421 agatgtctga atcattcttt ttatgttctt tgttggcaat aacaggagga tttttggata
    15481 tttatactta tgtggtaaga ggtaaagtct ttgctaatgc acaaactgga aatatagttt
    15541 tatttgggct taatatagca gaaggtaatg taaaggaatc attttattat ttgattccta
    15601 ttttggcatt tatgttaggt gtatttatag ccgagaagat aagaaagtat tttacaaata
    15661 ataagacaaa gattcattgg cgtcaggtta tcattataat tgaaatgatt acaatactta
    15721 ttgtttcatt tattcctaga ggaagattta atatgtttgt aaatgttgct atttctttta
    15781 tttgttcaat gcaagtagag agttttagaa aagtaaatgg gaatagtttt gcaacgacaa
    15841 tgtgtactgg aaatttaaga agtgctacag aaactttatt tcattatata catactaaga
    15901 atatttttat gataaaaaag agtttacagt attatgctat tattatattc tttatatctg
    15961 gagctatttt aggaactttt tttacaaaaa ttttcgtgga aaaatcaata ctaatatgtt
    16021 tttttatttt ggcagtggtc tttggaatta tgtttatcaa taaagatgat tcatttaatg
    16081 aaaattaact ttctgtaaat ttacaaaaaa atgaagctgt tatttgatat actttatata
    16141 tagcaaatat tgtatagatt aattaaagaa tttaaaatat agtaagcaat gtataagtta
    16201 atagaagtat tttaagtata gtaagcagtg tataaattaa ttaaaatgtc ttaagcatag
    16261 tagcaatata tagattaata gaagtatttt aagtatagta agcagtgtat aaattaatta
    16321 aaatgtctta agtatagtag caatatatag attaatagaa gtatttcaag tatagtaaac
    16381 ag
//
LOCUS       DAEMHZ010000091        16294 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000091 DAEMHZ010000000
VERSION     DAEMHZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.091"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..767
                     /locus_tag="KPK72_002610"
     CDS             117..767
                     /locus_tag="KPK72_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG2489811.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKTGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(907..2283)
                     /locus_tag="KPK72_002611"
     CDS             complement(907..2283)
                     /locus_tag="KPK72_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2489812.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2299..4212)
                     /locus_tag="KPK72_002612"
     CDS             complement(2299..4212)
                     /locus_tag="KPK72_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2489813.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVRISEESTKPNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4348..4980
                     /locus_tag="KPK72_002613"
     CDS             4348..4980
                     /locus_tag="KPK72_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG2489814.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5119..5658
                     /locus_tag="KPK72_002614"
     CDS             5119..5658
                     /locus_tag="KPK72_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2489815.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5834..6004)
                     /locus_tag="KPK72_002615"
     CDS             complement(5834..6004)
                     /locus_tag="KPK72_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2489816.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6289..7356
                     /locus_tag="KPK72_002616"
     CDS             6289..7356
                     /locus_tag="KPK72_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG2489817.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     NVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7526..9427
                     /gene="cooS"
                     /locus_tag="KPK72_002617"
     CDS             7526..9427
                     /gene="cooS"
                     /locus_tag="KPK72_002617"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2489818.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9420..9893
                     /locus_tag="KPK72_002618"
     CDS             9420..9893
                     /locus_tag="KPK72_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2489819.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKYNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9868..11094
                     /locus_tag="KPK72_002619"
     CDS             9868..11094
                     /locus_tag="KPK72_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2489820.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSI"
     gene            complement(11234..12421)
                     /locus_tag="KPK72_002620"
     CDS             complement(11234..12421)
                     /locus_tag="KPK72_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2489821.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNTRIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12694..13518
                     /locus_tag="KPK72_002621"
     CDS             12694..13518
                     /locus_tag="KPK72_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2489822.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVYKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13647..14912
                     /gene="gluD"
                     /locus_tag="KPK72_002622"
     CDS             13647..14912
                     /gene="gluD"
                     /locus_tag="KPK72_002622"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG2489823.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15125..15715
                     /locus_tag="KPK72_002623"
     CDS             15125..15715
                     /locus_tag="KPK72_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG2489824.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFTYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            16045..>16294
                     /locus_tag="KPK72_002624"
     CDS             16045..>16294
                     /locus_tag="KPK72_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG2489825.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNV"
BASE COUNT         6243 a         1946 c         2665 g         5440 t
ORIGIN      
        1 agaatgaatt tgtcaagttt aatttagttg taattgtatc ttttattaaa aataatataa
       61 attaaaacat ataaataaaa tattttatac aatatattgt aaaggaggta actttgatga
      121 aagcagaaaa aacaagtata tatccaatta ttaaaaataa tttaaacaaa atatcaattg
      181 ttgaatataa aaagggtcaa agatttgtag catcagataa agacttacaa gaagtgttct
      241 ttataataga gggagttgca ttagtggagt gtactactag aggaggaaat aaatttcttg
      301 ttgacattgt acctgaaaat gaatttatag gcaaaattag ttacatatat gaacataatt
      361 tgaaatgtga tatatttgca aagaccaata taaaattatt tcgatttgaa aaaaatgtat
      421 ttgaagagtt ttatctcaaa ccagatttta ttgcactatt tcacagaaaa tgtacaagaa
      481 gaatatatga attatataaa accagaatgg taagagaact attttcttgc acagaagtta
      541 ttgcctattg tatatcaaat aaacaagaaa ataatgtctg caatataaaa gctattcgta
      601 atttcagtga aatctattca attagtagaa aaagtagata ccattctctg ctaaagttaa
      661 ctgaaaagga gatagtaaaa aaaacgggta atacatatga aattttaaac tatgatgaat
      721 tacataaatt ggcatttgaa gtaaaagaat ttttggaaga tgagtaaaaa ttgttactta
      781 atcaaaataa agtataataa tttctgtaaa aaatagaaat agtctcactg aaaatcaaaa
      841 atactttagt tattttcaat gagactgttt agaaatattg tatacttata atgtatagta
      901 taattattaa tcaatttcct ttactatatt aatagcccat tttttagata atgctctagg
      961 aacaccaaat ataaacttaa ccacttcttc actataagtg agcaaaacga gtacaaatat
     1021 aggtgcacct ttaaatgcag ctataaaagt tagaggaata gaagctaacc acatacatcc
     1081 taattctaaa aatagagcat atttagtatc tcctccactt cttaaaatac caattataac
     1141 caatgtatta aaagatttaa gagccattag tattcccatt ataaagaata tcttagttat
     1201 atcagaagct aagctatcag aaacactaaa catttttaat aataatggta tatttaaaat
     1261 taaaataatt ccaagtatta aaccagctga aaatacaagt atagaaaact ttttagcata
     1321 ttcaatggct cttttaatat ggtcagctcc aagttcattt ccaagcataa tagatgcacc
     1381 agatgcaata catacagctg tcattatgaa gaaattacct gtactggttg ctatctggct
     1441 agctgctata gcagaagtac cagctcttgc atatgcaaca gaatacagga cagttccaat
     1501 tgcccataca gagtcattta caaatatagg aaagcttttt gatgaaaaag attttataaa
     1561 aattttatca atagctttta gattttttag accaaacttt aatatatagt cttttttata
     1621 aaggtaaaca taagtaatca ttaaaattaa ttcacatatt ctagcaataa cagtagctag
     1681 agcagcacct tttacaccca atgcaggaag accgaagtta ccaaatataa atccataatt
     1741 taaaataaca tttgttacaa gtgcaaatgc actacaaatc atacctaact taggatttct
     1801 aacacctctt gaaccagtgc taaatacagt acttacagca attaatggat aactaaatac
     1861 tgcaatacta aagtaatcta tacaaagttt taccacctca gaatcatacg aaaatatgtg
     1921 tataattggc tttggtgtta gtaaagcagg aataacaaat acaaaactta aaacaattgc
     1981 caatacacaa gtaagacctg tgacttttct tatattactc acatccttct ttccataaaa
     2041 ctgtgaaata aaaactccag cacctcctgt gataccagat aaggacatat taaaaagaaa
     2101 gaaaaattgg tttgcaactc caactgaagc tacagatgtt tcccccaaac tacttatcat
     2161 aattgtatct attacattta cagaagtaga aattaagttc tgtatgataa taggtataca
     2221 taaagataga agtattttat aaaatctttt attttcgtct tttaaagaaa atagttcttg
     2281 caaataatcc tctccttttt acataaattc ttcccactca tcatatagag aggcaaggtt
     2341 attttctaaa ttaatttttt cttggctaac ttctttagct ttatctggat tagagtatac
     2401 ttcttcttga cataaaagta tatctaattt ttcaatttca atctctaact tttcaatttc
     2461 tttttctata ttttgtcttt ttattctatt tttcttttct atttctcttt gttctctctc
     2521 ttttcttttt tcatctttta tttgagttct agttttttct tctttttctt ctataatact
     2581 catctcttgg atttgccttt ttttatctat ataataatca taatttccca gatattcagt
     2641 aattccattt tcatctaaaa ctaaaatttt atcaacaaca gtatttagaa agtatctatc
     2701 atgtgatatt gtaaatatag tcccatcata attagttaat gcctcttcaa ggacttcctt
     2761 agaatcaata tctaggtgat tagtaggctc atctaataac aataagtttg catttgatag
     2821 tataagttta agtatggcaa ctctagctct ttctccacca ctcaatgtag aaatcttctt
     2881 aaaaacttct tcatctacaa ataaaaatgc ccctaacata tttctcaaag tagtttgagt
     2941 aagatgagta ttattttccc aaatctcatc aattattgtg ttatctaaat taagagtttt
     3001 ttgttcctga tggaagtagg aaacatgtac attagttcca aatttaatat tgccactaag
     3061 aggttgtagt tcattagtta ttattttaaa taatgtagat ttgccaacac catttgcacc
     3121 tataagtgca actttttccc ctctatatat atctaaatcc aagtctttaa aaagtattct
     3181 ttctccataa cccatagata catctctaat ttgaagaaca tcattaccac tctggacaga
     3241 aggcgtaaat tgtatacgag cttttttcct ataagcttct ggtttatcta aaacgtctac
     3301 cttgtctaat gctttctctt tacttcttgc acgctttagg tgtttttctc taccataagc
     3361 ttttagtctt tcaattgatt cttcttgctt ttttatttct ttttgttggt cttcaaattt
     3421 cttaagctct agttcttttt ctattttaga taactcaaca aatttagagt agttaccatt
     3481 atatacttta agttttttgt tgtgaacttc aaaaattcta tttacggatt ggtctaagaa
     3541 atatctatca tgagatatta acatgacagt ccctttatat tgttttaaaa atacttctaa
     3601 ccattctatt gcttcagagt ctaagtggtt agtaggttca tcaagtaaca atagagttgg
     3661 tttcttaaga agtaattttc ctaaaagaac tctagttttt tcaccaccag aaagtatgct
     3721 tattggctta tccatatcat tgtcactaaa tcctaatccc tttagaactc ctttggcttc
     3781 agatttatat ccatatccat ttaaatctga aaataattct agtttatgtg aatattcatc
     3841 catgattttt tctataattg gagagtttgg ttttgtactt tcttcagata ttctaacctc
     3901 caattctctt aaataggatt ccatttctat caaattttta aaaacttcta gtacttcttc
     3961 caaaatagta ttatccgaat aaaaattggt attttgttct aaatatccga tttcacaatc
     4021 tttagaagtg taaatatctc cactatcata tccataaatt cctgaaatta ttttaaataa
     4081 agtagttttt ccagtaccat taacaccgat tataccaatt ttatcgcctt catttactgt
     4141 aaaactaata ttttctaata tagaatctat accaaaactt ttatttaagt tattacatga
     4201 caaaacaatc ataaatttct acctccttga actatctaaa attataatgt tttgatataa
     4261 tataatcaag gttatatgta ttagttttga aaaagaaata ttttattgtg aatcaaacaa
     4321 aaaagaggac acttttagga ggtgaagatg ttgggaaata aaaatatatc aatggcagtt
     4381 ataagaaggc tcccaaaata tcatagatat cttggagact tattagatag ggatatacaa
     4441 agaatatctt ctaaagaatt gagtgatata atagggttta ccgcttctca aataagacaa
     4501 gatttaaaca actttggtgg atttggacaa caaggatatg gttataatgt agaagctctt
     4561 catactgaga taggtaaaat tcttgggttg gatcgaccat acaacgcagt tcttgtagga
     4621 gcaggtaact taggacaagc tatagccaat tatgcaggat ttagaaaagc tggattcgag
     4681 ataaaagctt tatttgatgc aaatcctaga atgataggtt taaagataag agagtttgaa
     4741 gtattagatt cagatacttt agaagacttt ataaaaaaca ataatataga tattgctgta
     4801 ttatgtatac ctaaaaatgg agcacaagaa gttattaata gagttgtaaa agctggaatc
     4861 aaaggtgtat ggaattttgc acctttagat ttagaagttc cgaaaggtgt tatagttgaa
     4921 aatgtaaact taacagaaag tttatttacc ttatcgtatt taatgaaaga aggaaagtag
     4981 acaacttaaa catattaata atgagcagtt gtgaagagta ttgagaaagt cagaagattt
     5041 aatattaaat cctctggctt tttgaaatat taggataaaa atttaaataa tgtatggaaa
     5101 taaacaatat taaggagagt ggtttttgtg gaggaattat ggaaaaaggc aattgaattt
     5161 catggtcatg aatgtccagg attagctata ggatttaaag cagcacttgc agcaagaaaa
     5221 tatttaggat gtaaacagtc aggagatgag gaaattgtat gtatttctga aaatgatgct
     5281 tgtggaatag atggtataca agtcgtgcta agttgcacaa tgggtaaagg aaatttacta
     5341 cttaggatga ctgggaaatc agtatatcat ttctttaata gggaaaatgg aaaatctata
     5401 agaatatatg taaagtcaaa gccatttcaa atggatagag aaggatacaa aaagtactta
     5461 ttagaaagtg attttgaaga aatatttgaa attacagata ctaagttaag acttcctgaa
     5521 aaagcaagaa tatttaagtc tcttaaatgt gaagtgtgtg gtgaatttgc atctgagcaa
     5581 tatataagga ttgaagaagg aaagaaagta tgtcttgatt gctttaataa atatgaaaga
     5641 ttttatgagg agtattaaaa ttataaatta gattaaagtt aaaagtatta gatatgtgct
     5701 aacagtattt taagatgaat taggtagcaa cataataaag tatataaata atattaattg
     5761 aatcatacat aattaaaaag aggctgatta gtatttataa aataaaatca gtctctttat
     5821 gaataacgaa ttattattct ggttgaggtg catctacagg acaagctcct gcacaagaac
     5881 cacagtctat acaagaacca gcatctataa catatgcgtc gtctccagct gatatacagc
     5941 taacagggca ttcagcctca caagcaccac agcttataca agcgtcagta attttatatg
     6001 ccattttgaa atcctccctt ttatatgtat tgtaattcaa taacattata acaaatatta
     6061 ttgaatataa caattaaatt aattctacaa caaaaacttt attatgatat tcttgtttta
     6121 aagttttaca ataaagttta atctgcttat atttttattt attttaaaga agtttatttt
     6181 aaaaataaaa ttaaatttaa aaagttatgt attgattata tttaaaaatg ttatactcta
     6241 aaaaagtata ttttatttaa aaatttatta atacaaagga gatttactat gaaaaataaa
     6301 ttagtttaca tatgtattat gagtctattg ctatttgtag taggatgttc taataatcca
     6361 aataaggaaa gtaccattaa aaaagactct aagaataacc aaagccaagt taaagaagaa
     6421 aaaagagaac ctgtgaaaaa agatataact ataagtttcg ctggtgatgt aactatgggg
     6481 aactataaag gttcatcata ttatggcagt tttgaccatg aatttaaaag acaaggagaa
     6541 gattacaatt attttcttaa aaatgtaaaa cctgtatttg aaaaagatga cttaacagtg
     6601 gttaatttag aaggtccact tactacagcg tcaaatgcaa aagaaaaaaa atttgcattt
     6661 aaaggaaagc ctggatatgt aaatatttta aaatctggaa gtgtagaggc agtaagtatt
     6721 gcaaataatc attcagaaga ttattttgaa gaaggaatga aagatacaca gtttatattg
     6781 gacgagaata aaataaatta ttttggatta gggaaagatg ctgttgtaga tgtcaaagga
     6841 attaaagtgg gtctattagg gtataatgga ttatcaactg aatataatga aaaaaattta
     6901 aaacaaatgg aagatgatat taagtcctta aaaaagaaat cgaatgttgt tgtagtgtac
     6961 tttcattggg ggatagaatt agattattat ccagataaaa aacaaaaaga ttttgctcat
     7021 tatgctatag acaagggagc agatttagtt atggggtctc acccacatgt gattcaagga
     7081 atagaaaagt ataaaaataa gtatatagct tatagtctag ggaatttttg ctttggagga
     7141 aataagaatc cttctgatac agatagttat atatatcaac aaacctttac tttttcagat
     7201 aataaattaa cttcaattaa agagccaaat ataataccaa cttcaataac atctagtaac
     7261 tccagaaata attatcaacc aaaaatattg gatggaagtg agaaggatag agttttaaat
     7321 aaacttgaaa aaataagcaa agatttaaat aaataaaatg taaaaaaaaa taaatatata
     7381 tatttatttt aataatttat attttgttta gggtatggta aatcgtggta ttaaataagt
     7441 agattttaat agttaaatta tagtcaatta taataaagct atttaatcta cttttttaat
     7501 ataaaaatat aagggggaat ttgtaatggc ttgtaacact tgtaaaatgt gcgaaagtgc
     7561 agacaagaaa ttagaaagtt ttgtagcttc aaaggatgtt gaaacagcat ttcatagaac
     7621 agaagatcag aaagttaagt gtggttttgg acttcagggg gtttgttgta gactctgttc
     7681 aaatggacca tgtagagtaa ctcctaaatc accaagaggt atatgtggag cagatgcaga
     7741 cactatagta gcaagaaact ttttaagagc agtagcatca ggagcagctt gttatttaca
     7801 tgtggtagaa aatacagcta agaatttaaa aaatgttgga ataactaaag gtgttgttaa
     7861 aggagaaaaa acattaaatc aattagcaga gatgtttgga atagaaagtg atgaaaagta
     7921 tgataaatgt ataaaggttg cagacaaagt tatcaacgat ttgtataaat caagagatga
     7981 taaaatggaa ttagtagaaa aaatagctta tgcacctaga gtgaaaaagt ggaaagagtt
     8041 aggtataatg cctggtggtg caaagtcaga agtttttgat gcaatagtaa aatcatcaac
     8101 aaatttaaat agtgacccag tagacatgct agttaattgt ttgaacttag gtatatcaac
     8161 aggattatat ggtcttacac taactaatct tttaaatgat gttatgttgg gagaaccagt
     8221 tataagaatg gctcctgtag gatttaatgt aatagaccca gactacataa atataatgat
     8281 aacaggccat caacattcaa catttgcaaa tttccaagat agattaaaag atgaagatgt
     8341 tataaaatta gctcaatctg ttggtgcaaa aggctttaag ctagtaggat gtacatgtgt
     8401 tggtcaagat ttacaattaa gaggagaaca ttatcaagaa gtatttgctg gacatgcagg
     8461 taacaatttt acaagtgagg ctgttttatc aactggagca atagatatag ttctttcaga
     8521 atttaactgt actataccag ggcttgaacc aatagcagat aaatataaag taaaaatggt
     8581 ttgtttagat gatgttgcta aaaaagcaaa tgcagaatac ataggtttag acagaagtaa
     8641 attagatgaa cttagtaaca ctttaataga aaaagcattg gagtcttata aggagagaag
     8701 aggaagtatt gaaatagata taccaaaaga ccatggattt gaacaatctt taactggtgt
     8761 aagtgaaaag aacctgaaag cgtttttagg agattcatgg aaacctctta ttaatttaat
     8821 tgcagaagga aaaattaaag gtgttgcagc tgttgtagga tgttcaaata tgacagcagg
     8881 aggacatgat gtaaatactg ttgaacttac taaagaattg attaaaaaag atataatagt
     8941 actatctgca ggatgttcta ctggaggatt agaaaatgtt ggtcttatgt cacctggagc
     9001 agaagaatta gcaggagaaa acttaaaaga agtatgtaag actttaggaa taccaccagt
     9061 attaaacttt ggaccatgtc tagctatagg aagactagaa atagtagcaa cagaattagc
     9121 agctgaactt ggaattgacc taccacaatt accattagtg ctttcagctc cacaatggtt
     9181 ggaagaacaa gcattagctg atggagcgtt tggtcttgcc cttggcttac ctcttcattt
     9241 agctttacct ccatttataa caggaggaaa attggtgaca gaagtattaa cagaaaaatt
     9301 aaaagactta acaggaggac atgttatagt gaatccagac cctaaatctt cagctaatca
     9361 attagaagaa ataataatag atagaagaaa aaatttaggg ttaaaggatg tggaatgcaa
     9421 tgcatagaat ttttataaat aaagatttat gtacaggatg taagagctgt gtactagcat
     9481 gtatgctaaa atacaataaa gattatgaca tgtacactct tgatttagaa aatatagata
     9541 atgatagtag agggcatata gaattagata gtaaacataa taatccagtt ccaattcttt
     9601 gtagacactg tgatgaacct gaatgtgtat tagcatgtat gagtggtgca atgcataaag
     9661 atagtgaaag tggaatcgtt tcatatgatg aagagaaatg tggttcatgt tatatgtgtg
     9721 taatgtcttg tccatatgga ttattaaagc cagatgatag aagcaagcag aacatactta
     9781 aatgtgattt atgtaaagat gaggagtatc ctagatgtgt agctaattgc ccaagtggag
     9841 caattgaatt acagaaggag gaaaatgatg aattatgtag tgttaggggc tagtgcagct
     9901 ggtataaatg cagtaaaaac attaagagaa ttagataaag attcaaatat agtagtaatt
     9961 tctaaagatg aaaacgtata ttcaagatgt atgttgcatc atgttatatc tgaacacaga
    10021 actttaaaac aaataaattt tgttgatgaa gattttatgg agcaaaataa tgtaaaatgg
    10081 attgcaggta agactgttaa aggtatagac ataaataaaa aggtagttca gacagaagac
    10141 attactgtaa attatgataa acttttaata gctacaggtg catcttctgc tattcctcct
    10201 ataaaaaatt taagagatgg aaattttgta tattctgtga gaaacataga tgatatttat
    10261 aaaataaaag aaaaagcaca aaattctaaa aatgtagtta taataggtgc tggtttagtt
    10321 ggtatagatg cattagtggg attatttaaa tatgagcagt taaatatttc tgtagctttt
    10381 atggaaaagt acattttaga tagacaattg gatgaatata cagcatcagt gtatgaaaat
    10441 aagttcaaag aaaaaggtgt aaaattttac ccaagtgcat caatacaaga aattgtattg
    10501 gataacagta agaatgttac aggtgttgca ttttcaaatg gtgaagtttt agatgcagac
    10561 atggtaattg tagctacagg ggttaaacca aatgctgact ttttagatgg aacaggaata
    10621 gaatatgata gaggtataat aatagatgat atgtgccaaa caacacaaaa agacatatat
    10681 gcagcaggag atgttgtagg taaaaatgct atttggcctc tggcagtaaa gcaaggaatt
    10741 gtagctgcct ataatatggt tggaaaagat aagaagatag aagatgaatt tgcttttaaa
    10801 aacagtatga atttcatgga tataccaact atttctatag gaatgaatac acctgtagat
    10861 gatagctata aagtattaac cagacatgga attaatgatt ataaaaagtt tgtttttaaa
    10921 gataatgtaa tctatggagc agttatacaa ggagatattt catatgtagg tgtattaact
    10981 tatttaataa aaaataaagt tgaaatatat gatttagaaa ataggatatt tgatgtatgt
    11041 tatgcagact tttttaatat aaaagaaaat ggagaatttt gttatagtat atagtatata
    11101 cgtgaagtaa ggtttataaa tacagtaaat caataaatag agttattcta aaataagaga
    11161 aatgacaaat cttatttaga aatagctcta tttttttgca attaaattta aattatattc
    11221 aatttgtaaa atattatttt gaacaattat acatatactc tctagttaga ggtaaatcat
    11281 tttttatacc tttagtgaaa agtatttgat gtatatctat aataccagtg ttgaaagcag
    11341 cagcacaaga acaaaggtat aaatcccaca ttcttataaa cttatcatca aacatttctt
    11401 taatctttgg aagattctca gagaaatttt tttgccagta tagtaaggtt tttacataat
    11461 gtaatcttag actttctacg tctatagtat gatatttgta atcactacaa atatgtatca
    11521 tttctcttag gctaggaata actcctcctg ggaaaatata tttttttatc caagggtctc
    11581 cataagattc ctgcaatcca cttatatagt gaagtaaaaa caatccacca tcttttaaaa
    11641 cagcatttac gtttttaatg aaaagttcat agttttctct accaacatgt tcaagcatcc
    11701 caacgcttac aactctatca aacataaggt ctgatttttt taattctcta tagtccataa
    11761 gtcttacctc aagatagtct tgaaggttct cttctttaat tcttgtgtta aattccttaa
    11821 attgctcttc gctaagtgta attccaacgc cgtgaatttt atattttttt gcagcttcta
    11881 ttagtaaaaa tccccaacca caaccaatat cgagaaggct cataccttcc tctaaattta
    11941 gtttagttaa tatgtggtga actttattga cttgagcatc ataaagagta tcattatcat
    12001 ttctaaagta tgcacaagaa taactaagtg tcttatctaa ccataagcta taaaaatcgt
    12061 tgccaatgtc atagtgggca tgtatttctt tcttttgatg tctcattgaa tttgatgagt
    12121 gcaatagatg ttttacagct gaattatcaa tattgaattt gtctatttgt tttaaaagta
    12181 gatttaaagt taagaataaa tctccctcaa cttcaatgtc tctatttatg taggcttctc
    12241 caagtgctaa agatgtactt tttaatagtt cagacttgct aatatcatta tttactataa
    12301 ctttgaactg aggggagccc tctccaatta gaaattcttc ctcatcttta agcctaacta
    12361 gaaatggtgt ttcaccaaat ttctctaaat agtcttttaa aaaatttttt gtaatattca
    12421 tattaccagc tccttctaat gtaaatccag taagtttccc tgcttaaaat atattatagt
    12481 ccttttttta aaaaagtaaa attttttcaa taaatttttt atttaaagga aatttatttt
    12541 ttgtaaagaa atttcatgtt gtagtatatt ttttgcttat aaattttggt actggatata
    12601 atattaaact gttaatgata taataaattt aatctataaa gtaaataaaa ttttgtaata
    12661 aatagttaaa gctattttat aaggagatat atgatggaag tatatgcttt ggttgggtca
    12721 agtggcacag ggaagagctt taaggcttta gagttcgcat atgaaaatga tatagaatat
    12781 ataattgatg atgggatttt aatatacaaa aacaaggttt tagctgggat atcggcaaaa
    12841 caagcaaata ctactataga agcagttaaa agggctattt tttataatct ggagcataga
    12901 caagaagtta gagaagcaat taaaaaagaa aatataagaa aaattcttat tattgggaca
    12961 tcaaaaaaaa tggttaatca aattgtagag agattgtcaa ttggaagggt ctataagttt
    13021 ataaatatac aagatataag tactaaatat gaaatagaaa tagcaaaaca agctagaaaa
    13081 gaaggaaatc atgtaattcc tgttccagca gttgagataa aaagtatggc aagtggtctt
    13141 agtataaatt ctttgaagag actttttaga aaaggaaata ataggaatat ggtagtttta
    13201 gaaaaaacaa ttataagacc aacttttagt tatataggaa agttttatat tagtgctgat
    13261 gtaattaaac agattattga atatgaagta tacaactttg gaagcattga tagaataaat
    13321 aaaatcaata tagagaattg caataatttt atgaatattt ttgtaagtat aaatatcaat
    13381 gatttagaaa ctataaaaaa aattgaagac atacaacaga caataaaaaa gtctattgag
    13441 aaaatgactt tagttaatgt acaaaaaata gatattaaca ttcataaaat taaaaaaata
    13501 aacttcctcc aatgttaaaa aataaactga gaaaatgata tactaatttg caataaagtg
    13561 tgctataatt aaactgtaaa tttaggaaat ggttttttct aaatggagta tacataatta
    13621 taacgtattt ataagggggc tttcaaatgt caggaaaaga tgtaaatgtc ttcgagatgg
    13681 cgcaatctca agtaaaaaat gcatgtgata aattaggtat ggaaccagca gtttatgaat
    13741 tattaaaaga acctatgaga gttatagagg tttcaattcc agtaaaaatg gatgatggtt
    13801 ctataaaaac ttttaaagga tttagatcac aacataatga tgcagtaggg ccaacaaaag
    13861 gtggaataag attccatcaa aatgtttcaa gagacgaagt aaaagcttta tctatatgga
    13921 tgactttcaa gtgttctgta acaggtatac catatggtgg aggtaaaggt ggaataatag
    13981 tagatccatc aactttatct caaggtgaat tagaaagatt aagtagagga tacatagatg
    14041 gaatatataa attaataggt gagaaagttg acgttcctgc accagacgta aatactaatg
    14101 gtcaaataat gtcttggatg gttgatgagt acaataaatt aactggacaa agttctatag
    14161 gtgttataac tggtaagcca gttgaatttg gtggttcttt aggaagaaca gctgctactg
    14221 gatttggtgt tgctgttact gcaagagaag ctgctgctaa attaggaata gatatgaaaa
    14281 aagctaaaat agctgttcaa ggtataggaa acgttggttc ttatacagtt cttaactgtg
    14341 aaaaacttgg tggtactgtt gtagctatgg ctgaatggtg taaatcagaa ggttcttatg
    14401 ctatatacaa tgaaaatggt ttagatggtc aagctatgtt agattatatg aaagaacatg
    14461 gtaacttatt aaacttccca ggagctaaga gaatatcttt agaagagttc tgggcttcag
    14521 atgttgatat agtaatacca gctgcattag aaaactctat aactaaagaa gttgctgaat
    14581 ctataaaagc taaattagtt tgtgaggctg ctaatggacc aactactcca gaggctgacg
    14641 aagtatttgc tgaaagagga atagttctta ctccagatat attaactaat gctggtggag
    14701 ttacagtttc ttacttcgag tgggtacaaa acttatatgg atactactgg tcagaagaag
    14761 aagtagagca aaaagaagaa atagctatgg ttaaagcatt tgaatctatt tggaaaatca
    14821 aagaagagta caatgttaca atgagagaag ctgcttacat gcattcaatt aagaaagttg
    14881 ctgaagctat gaaattaaga ggatggtact aagatttact cttaataaat aataggatat
    14941 aattatttaa gatataaagg ggctgtacat agtacagccc ttaaattatg tggcttacaa
    15001 tgaagtaagc tatttttgtt ttaacttata aagaacttga taaattatgt aatttagaat
    15061 gtatttttta ccaccacatg attacaaatt agaggagtta gattgaattt ttagacatga
    15121 acttatgcat tttaaaagca aagaccttaa tttaaagtat ctagtattat ttttaaaaat
    15181 agtatattgg tttaatccat ttatatgtat aatggaaaaa tatatggatt tggattgtga
    15241 actttattgt gatgagagag ttttaaaaaa ttgtactgta caacaaaagc aagaatatgc
    15301 tctaactatt gtaaattcaa tgaaaagaag tacaaatttt tcaaataaat ttgtagctgg
    15361 attaaataaa caatctgata ttagaaaaag agtaataaac atgttcagtg aaaaacataa
    15421 aaatggtatc ttaatggctt tgattataag cttgttttct tctattacat tttttaaatt
    15481 cgattcaact agtattattg aagagaatga tttcttagag aggtcaagat atacgtttga
    15541 aggaaggcaa gaagatatgt ttgtaacaat agattttaca tatgcagatg cacctataaa
    15601 acttagaaaa gagcatgaag aaaactgtag agtcaatgga atagtaccaa aagattcaga
    15661 ttcaatagaa attattccta gattttataa agattgtatg gatttaaaaa aataagttag
    15721 acttaatatt gatgttgata ttatgaattt tttatattta agcaattgaa aattttatta
    15781 tagattcaat tttatatgtg tttaagtatg aaatttatag atagttgaaa taattaaata
    15841 tgattgatgc ataggaatca ttactaagct ataattaatt ataggggagt ttaaataaat
    15901 tatgagttac acaattataa aatgataaaa atagttatct gttacaaaaa atgtatcttt
    15961 ttaattaata aagataatat gaaaacttaa aaggcaaata aacttaaaca taaaatactc
    16021 ttaaataaga taggagaaaa atgtatgtct aactttattc taaatgggat ttttagaaca
    16081 gttatagtaa gtagtctaag cattcttatg atattgattt tcaagaaaaa tgtatttaaa
    16141 agatttagca aaaaatttaa ttattatata tggatgatag ttgttattaa attattttta
    16201 ccatttacat attatacttt tacaataaat atcttgagaa gtaaaaaaca cattaatatt
    16261 aataacataa atttagaggg atttaataat gtct
//
LOCUS       DAEMHZ010000092        16225 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000092 DAEMHZ010000000
VERSION     DAEMHZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.092"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..340
                     /locus_tag="KPK72_002625"
     CDS             59..340
                     /locus_tag="KPK72_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489826.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            604..858
                     /locus_tag="KPK72_002626"
     CDS             604..858
                     /locus_tag="KPK72_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489827.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            903..1226
                     /locus_tag="KPK72_002627"
     CDS             903..1226
                     /locus_tag="KPK72_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489828.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1785..1979)
                     /locus_tag="KPK72_002628"
     CDS             complement(1785..1979)
                     /locus_tag="KPK72_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2489829.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2343..3962)
                     /locus_tag="KPK72_002629"
     CDS             complement(2343..3962)
                     /locus_tag="KPK72_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2489830.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(4271..4510)
                     /locus_tag="KPK72_002630"
     CDS             complement(4271..4510)
                     /locus_tag="KPK72_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489831.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            4781..4972
                     /locus_tag="KPK72_002631"
     CDS             4781..4972
                     /locus_tag="KPK72_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489832.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(5061..5873)
                     /locus_tag="KPK72_002632"
     CDS             complement(5061..5873)
                     /locus_tag="KPK72_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG2489833.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            6295..7446
                     /locus_tag="KPK72_002633"
     CDS             6295..7446
                     /locus_tag="KPK72_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2489834.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRMRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVNFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            7564..8202
                     /locus_tag="KPK72_002634"
     CDS             7564..8202
                     /locus_tag="KPK72_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2489835.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSENIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            8203..9225
                     /locus_tag="KPK72_002635"
     CDS             8203..9225
                     /locus_tag="KPK72_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG2489836.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            9215..9835
                     /locus_tag="KPK72_002636"
     CDS             9215..9835
                     /locus_tag="KPK72_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489837.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9959..10645)
                     /locus_tag="KPK72_002637"
     CDS             complement(9959..10645)
                     /locus_tag="KPK72_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG2489838.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10659..11204)
                     /locus_tag="KPK72_002638"
     CDS             complement(10659..11204)
                     /locus_tag="KPK72_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2489839.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            11432..12679
                     /locus_tag="KPK72_002639"
     CDS             11432..12679
                     /locus_tag="KPK72_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2489840.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            12766..14760
                     /locus_tag="KPK72_002640"
     CDS             12766..14760
                     /locus_tag="KPK72_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2489841.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNAFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            14760..16082
                     /locus_tag="KPK72_002641"
     CDS             14760..16082
                     /locus_tag="KPK72_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2489842.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         6460 a         1744 c         2439 g         5582 t
ORIGIN      
        1 tatttttaag aataaaattt attattaaat aaaaaataaa ggagagtgat ttaatagcat
       61 ggcatcaaaa caaataccaa gaaacaattt agaaaagttt ttaagaataa gagtcttatc
      121 acatacaggc gataaaatac atataaagtt acctgtaaat tttgcaaaga gaatgattga
      181 gaataatgct ttggatttat ttaatggaaa agatgatgta gtagatggta aaaaagttac
      241 tgaaatatta gttaaggcat ttgactatga tttaactgga gaagtagtcc acttagagag
      301 aaaaaatgga gacattatta aagtttcaat tgtacaatag aattatttta tatgaatttt
      361 atttttgaat agttataaac aattagaaat ttgtaaaaaa gtataatata ttattagtaa
      421 tggtgcagaa tacacattta taatttatag atgtgtattt tttgtttaaa aaatttaaat
      481 atataaaact ataaaggtta caattttgga aatagatgta aatatatgtt gtattgtata
      541 ataagagtaa tataaaatct agtaaaaata atcaaataaa tttagcttat gagagggata
      601 actatggaaa ataaggattt taaattaaca atatgtatat ttgcttttgt aattggagta
      661 gtagtattat tctgttgtat aggtttaggt gaatttagtg cagagcatat tattcaacaa
      721 aatggtggaa tcatggatac tgaccaatat aatatttatc tagaccaatc tatttctcaa
      781 tatagaaatt ttggttctat attagctctg ttaggaggaa tagggatatt gtttgataaa
      841 tatatcagaa aatactagag attatgtttt gtctaaaata aattcttatg aggagataaa
      901 aaatggcaac agaaatattt actgtaaaaa ttacaccctt agaggcacaa aaaatgataa
      961 aagaaaatct tgatgcagat ttagttttta gtgattctta taatttagga gatgataaat
     1021 ttatactaat tacaactttt gagaagtatt atgcaagaaa taatagtgat gcaggattaa
     1081 tagtagttta tgaaaatacc acaggaaaaa cagttattaa agctacttca acaggcagtg
     1141 caattggaat tttaaaaatt gattggggtg caggtggaaa tttacttaaa agagtaaaaa
     1201 acatacttgc tgattatatt atttaattat tttatctaac tgataatatg tcaagaaaat
     1261 ctcgtaaaag aggttaagaa catttttata atatatagat aaataagaat ttgaaggtaa
     1321 agtttttaag gtataaagaa aaataatata ataaaacgaa ttggctttat aattctttat
     1381 aaggcaaaga gcacatttct ttatataatt gttggatatt aaataatatg taaatacacc
     1441 atttacatat aaaagtttaa ttaatatgtt aagagaaaat gaaatggaaa ttactaaaaa
     1501 atatttttaa attggataat ggttgatatt atgtttatac aaaaatcatg tagaatttta
     1561 tatattttaa atatgatagt ttaaaattat gaaagaatga taaattccag tttgtagggt
     1621 aattttgata aataatatta ataagtatga gtaaaaagta ggagcatatt aatataatgt
     1681 ccctactttt ttattattaa aattgtttat aatatcaaca ctacattaaa acacaaccta
     1741 aatttgaaaa acaaatagtt tagaaatatg tactttttta tctattatat tcttttaaat
     1801 cgaatttttt catctgaaca taaagtctca aatctatatg cgtctgtcat atcataaaaa
     1861 ccaaaaaata taaagccatc acgagaagta aattcatatt gtatattttc aattttcaac
     1921 atgttttcta cttcgcttat tttttcgcta tatataattt ctaccatata atttatcatt
     1981 gtagaatccc tcctattaat atgaaaatat ccaaatatat aataacatat ttagatgata
     2041 taatagtatc caaatagaaa ataatcttat tacattattg aatgaagaat taaaatgaag
     2101 ataaataaaa caaatattgg ttataattga ttaaaaaata cttgtgactt tagttatggg
     2161 cagttcaagt tttatgaaag aagtcatata tatatggaat attctaggca taaactgttt
     2221 aataataaga gaatactata aaaatttgag tattacataa aaaataagaa gccataaatg
     2281 atataagcat tatctatagc ttcttacttc tcattgatta agattatata taaaaataat
     2341 cactatattt tttgtttaag ttttagtact tgttttacta tatttgttga gtggtcagaa
     2401 attctttcta gattagtaag taagtcaaga taaagtacac cagcatcaat tgtacaactt
     2461 ccattattta gtctaatcat atgtgaattt ctaaaagttt tttctaaata atttacttca
     2521 ttttcaactt ctaaaacttt atttgctaac tcaaaatctt taacatctaa aactattaag
     2581 gcatccttaa aattagtaat tgtcttattg tatatttcgt atatttctct tatcgcacta
     2641 tcagaaaaac taatgttcat ttccttaaca ctcatagcca attcagctat attttctgca
     2701 tggtcagaga ctctttcaat gtcatttaca gtattaaaaa gcaaatctgt tctaaagcgt
     2761 tcatcatctc gaagaggttc tttagaaagt tttaacaaat agttcaatat atctttctgt
     2821 aacttattga caactttttc tgactcaaaa gctttattaa tatcttcatt gctcttattt
     2881 aaaaatcctg caattgaatg ttccaaagag tttgtagctt tatgacccat tcttacaact
     2941 tcttgtacag tattaccaag tgctatagat ggagtgctaa acatcctttc atctaagtac
     3001 ttagtattat ttactatttc tgattcagtt tctttaatag gtataagtct atttgctaat
     3061 ttaactatgt atttagaaaa tggtaataat attataacat ttgtaagatt aaataaagta
     3121 tgtgcatttg ctatttgtct agctgtatca gttgggtcaa aatgagtaac tatggctgat
     3181 ataggtttat ttaaaacaag cataaatatt attgtaccaa ttacattaaa acttaaatgc
     3241 attacagctg cacgtcgagc atttctactt gcaccaacac ttgataaaag tgaagtaaca
     3301 caagtaccta tattatctcc ataaagaata ggtaaagcag atgatagagg aagtattcct
     3361 tgagaagcta gtgctattaa catacccata gatgcacttg aactttgaac aataccagtt
     3421 atggcaaatc ctgccaatat acctaataca ggatttttac taaaatataa aagtgcatca
     3481 gtgaatgctt tgtattcagc aagtggagct actgcatctt tcataaactc cattcctgta
     3541 aataatatac cgaaacctat aagtatctca gataaatgct ttgttttttg atttgatgta
     3601 aataaataga ataaaatacc tatacctaaa gcaattggtg caattccttc aagattaaat
     3661 gaaactaatt gagcagtaac tgtagtacct atatttgccc ccattattac accaatagct
     3721 tgacttagat tcattatacc tgcatttaca aatcctacaa ccataacagt agtagcactt
     3781 gaactttgta taatacctgt aacgactgta cccaccagta cacccataac aacattactt
     3841 gttaagagtt caataatttt ttttaatttg tctcctgctg atttttgtaa accttctccc
     3901 attaaactca ttccgtaaag aaaaagtcca agtccaccga taaggctaat tactatattc
     3961 acgatatttc ctcccttaag aaattaataa tttttttatc tgaattataa actttattaa
     4021 ctaaaattta ttgtttccat aatatagaat aacaaatttt gtcaataaaa atattaatgt
     4081 tttgttaagt atacgttaag agaatgttaa atttttaagt tgttgttaat tttagaaaat
     4141 ttatgatata tagcaattta aatacttgaa cttagatttt tagtatttta tgtacgatca
     4201 atttaacaaa gaattaacta taattgtaaa aaatatgtaa aaaaataaca tccttttaga
     4261 atgatatttt ttagttataa atatttttat attcaattgg tgtaaagtta ttaaactttt
     4321 taaaaattat actatagtaa ttttgactat tgaatccaat tagtacagca acgtctaaaa
     4381 tagacaagtc tttttttaat atatactttt tgcttttttc aactctaact ttacttaaaa
     4441 aattagtaaa agtatatcca ctttttttct taaacaaaga aaaaataact tttattaagt
     4501 tgtaattcat tacatatatt acttactgtt atatcttctg agtaattttt gtgaatataa
     4561 tctatagttt tctttaagtt ttctttacat aaaactaaga gttggtctat gtttcatttt
     4621 atcaaggcat atttattaaa aaatcaatct ctatttgtta aaataattta tgaatgatgt
     4681 tagaaactgt aatatattgg atataatata ataaacagat aaactctgta ttaagaggat
     4741 atatagttta tcttaattat aggggaagtg aggatatatt atggaaagat taatggaact
     4801 catagaaaga aaatttttaa aggatgtaga acttttagaa ttagaagaca tagaaaatgt
     4861 aatagaatgc aaaatgacac agccatataa tgctcattat aatgaatatt tagtaaaagt
     4921 aaaaaatgga gatgaagtag aagaatattt tgtgtattta aaacatagat aaacataaaa
     4981 gaggtttata tttatttgta tgattataaa ataaatacaa gcctcttttt ttgataaata
     5041 taaaattata aaatttttat ttatttaatt ttttcaacaa cattagtcaa tattgaatct
     5101 aaatcatcac taatttctgt aactccatta tgagtaatat ctataatttc tttagtcata
     5161 ggaagttctc ctaaaagctc taagtctaaa tcatctaaaa acttttcagt agattctcct
     5221 tcaaataact tgattttttt agaacaatca ggacattgga tataactcat attttcaata
     5281 actccaagga catttatgtt cattttttta gccatattaa cagcttttga tacaatcata
     5341 gataccaaat cttgaggtac tgaaaccata actattccac ttataggaat agattgcata
     5401 actgtcaagg caacatctcc agtaccagga ggcatatcta ttaataaata gtctaattct
     5461 tcccaaagta catcagtata aaattgcttt acaacccctc caagtaaagg tcctctccaa
     5521 acaacaggtt cattttcatc atctatcata aggtttatag acattacttt tatgttattt
     5581 gagttttcta cagggtaaat ataagagcca tcagaataag cttttacatt ttttacaccc
     5641 ataagtctag gaatacttgg tccagttata tctgagtcta atataccaac cttatatccc
     5701 attttgttta gcttattagc aagtaaagca gttacagttg attttccaac tccacccttc
     5761 ccactcataa caccaattat ttttccaaat ttattgttag ggttattttc aatagaacaa
     5821 tttgattgac tattacaatt tccctttgat ggacatgaat tacaatctgc catatctatt
     5881 acctccaaac tttaatattt attagattat ttgtaaccta catatactat tttagtacaa
     5941 ttgaaccata aagtcattat ttaaaagcaa cttagaagat tttctattga tttattcata
     6001 gactatagca aaaagaatac aataaaaaaa ttataacatt cttgaaaaag tgattatttc
     6061 aatttagtta tagaacttag tcatataaat atatagaaaa tattttaata tattatacat
     6121 aacgggttat tttataagtt taactataaa ttaggtttat attttataaa aatttgctaa
     6181 aaaaaacaat taaaaattgt aaagaaaaaa gatataatta gaactgtagt aataaatgtt
     6241 ttattataga taatagagtt ttaagtatct taaataatat ttggaggaat atgaatggaa
     6301 ttaagaccaa gaagtgcaaa attagaagaa tttgataaag taatagaatt aataaactat
     6361 gtatttagaa tatcaagaaa tcataaacca actatgatgg aagaatttcc tcttttacta
     6421 agtaaaaata atatagaaaa tatgattata atatcagaag atgataaagt ggtttctgat
     6481 gtaaactatc taattcagga tgtatctatt caaggaaaca gattgaaggt tgcagctata
     6541 ggtggagtat gtactcatcc agattatgaa aagagaggct attcatctaa gatattggat
     6601 aaagtagaag aaaaaatgtt ttatgatggt gtagatattg taattatatc aggaacaaga
     6661 agtctataca gtagaagaaa ttgtagtttg gtaaagagtt tttataaata tacaataaaa
     6721 cctgaagatg taaaaatagc atatgaaata gttgaatttg atgaaactaa ttttgaaaaa
     6781 gataatgatt tagataaaat gattgaacta tataatcaaa atagtactag atttataaga
     6841 atgagagatg agtttcaaaa actattgcat gcagcaacta ttgcctgggg accaattggt
     6901 tacaagaagg tatttataaa ggaaaataat aatataatag gatatttgat aataagaaca
     6961 ataaaaaaag aagattctac tgtaggagaa gttgctgaaa tagggttaaa ctctgtaaat
     7021 gttgaaaata tattgaaata tgttgcaaat aaatttggat tagaatattt aaattataaa
     7081 gtacatgtga aaaatcttaa agaccagttg aaatgtaatg ggacaaaatc tttagactat
     7141 caacaaggaa ctatgaaaat aataaatttt actaagttgt gtgatagttt aagaagttat
     7201 tttagtcaat atgtaaattt tgaattacta aaatacatgg aatttaaaca agttgaaaat
     7261 aaatatatta ttaaatacaa agaagaagaa cttgtaatag aaaatttgga taagcttaat
     7321 aaactgtttt ttgaaaaaaa tgaggaacag tacaatgaat ttaaacatct aaaaaatata
     7381 tatgaatttg ctacaaaggc atttcctgta gattttccat ggactgcaaa tttaaattat
     7441 caatagtttt agatgatatt aaataaaatt gattgatacc tagagttagt ccttgttaaa
     7501 taagagaaat tattggatat tgaaaaagca attttgtaag taattagaaa ggatgagtat
     7561 tatatggaca tacttgatga tatagcagaa tgtatattaa atatgggtgt agataatata
     7621 gagaatttag taaaaattgc tatagataaa aatattgatg tggaagatat ttatgaatat
     7681 ggtttaaaca aaggtatgat aggagcttta gataagtttg agaataagga atactatctt
     7741 tcagaagtta tagtatgcac agatgcatta aataaaggta ttaatctttt gaagggaact
     7801 ggaaaagtaa agaaaaaatc aaagggagta attctgatgt ctgtagtaga aggagatact
     7861 catgagatag gaaaaaatat tgtaaaagta atggtagaag ccactggata taaggttatt
     7921 gacttaggtg taaatagaaa aagtgaaaat ataatagaag aagctataaa aaacaatgta
     7981 gatataatag gcttatcttc aatgatgaca actacaatgg aaaatatgaa atcggttata
     8041 gacgaactta atatgattga gatagataaa agaccaaaag taataattgg tggaggacct
     8101 gtaagtatgg agtttgcaga agaaatagga gcagatggat acagttcaaa tgctccaaaa
     8161 gcagttaaat taataaataa acttatagga ggtgaagttt aaatgttttc agatatgact
     8221 ttacttcagt atataaatag tgaagataga ggtttttttc tgcctgatat gggtacaaat
     8281 ggattgttct taataaataa aaaggcatat gaagtatatg atagtgtaga tttgcaatta
     8341 gaattagcaa agactatgga caagtatttt gaatcagatt ttatatactc attctgtgat
     8401 gggattacat tttgtgaaac tctgggttta gagacattaa gaccagatta tgattttcca
     8461 agtgtattga cacatactat aactgacaga gataaattaa gaaaattaga tgttccagac
     8521 ccttatacga gtggacgtat gccactcaat atagagagct tatcagttat atcaaaaagt
     8581 attaaaaaac cactatatga atcgatacaa ggtccattta cacttgcagg tcaattagct
     8641 ggagctactc aacttttaag atgcataata actgataaaa aatttgtaga agaattattg
     8701 gaatttacaa ctgaattggt aagaagatat gctgtagctg caaataaagc aggagcaaaa
     8761 tatatttcaa tagcagagcc aacatcagta accctaagta aagatagatt tgatgaattt
     8821 atagtaaaaa atttaaataa aatctacaat gaacttgatt gttggaaagg tatgcatata
     8881 tgtggagaca ccagagaatt attagataat atgctaagtt gtaatataga tgcagtaagt
     8941 ttagaccaaa ttttagacta tgaagaaatt gctccaatga ttcctagaga tatagttttg
     9001 attggtaatt tagacccaat aaaacttctt ggacgttcta agccagataa gattagaaga
     9061 gagacgctaa aattgttaaa gaagatgaga ggatatgata actttttatg tgattttggc
     9121 tgtaactgct taaataccac tccagtagaa aatttacaag cagctataaa ggcaggaaga
     9181 attagttata aagaattaga taggattgat attgatgaat tataatagta ataaaaataa
     9241 atatctttta aaggatataa atttagatga tatttttata gataagaata ttgtgctaaa
     9301 gtttttgggg tatggaaata gaaaagctcc agatacaatt agtaaaatcg ttgaaagaga
     9361 aataagaaat ataaatgata ttttagatat caagatatat ataagtgaag ttgatattaa
     9421 taaaacacct aaagaatgta aaaaagcatt tgctgtacta tatacaatag gggataaaat
     9481 tgattttaag atgaatgact atatggaaaa ttgcaatatg atggcagggc tagcattgga
     9541 taaaataggt atagtatgtt tagattatat taatgagaaa ataaaaatgt atttaaaagg
     9601 aaagtatgat agtctcaaaa tatcgcatga aatatatcca ggagataaag attttgaagt
     9661 agagagacaa aaggatattt ataactatgt aaaaaataaa tataatagta tagaaataga
     9721 aatcaatgat tatcatcaac tatcccccat aaaaagtgtt gcaatgctta ttttaatggg
     9781 ggatgaagaa aatttagaat caagatgtag taagtgtcct agaaaatgct tttagttaat
     9841 tttcaagact aagatttgtt ttgtgtataa attgcaataa tttttttggt cattttatgt
     9901 ataaactaaa aaaattattg caaagtaata ttctatttaa tttgaacaga cttacaaact
     9961 atagtagttt ctttaattca ataagtgtat ttatttcata agttggtata tgattagtat
    10021 aatttatact gttattggaa tttaaccaac aagtatctat tcctgaattt attccaccta
    10081 agatatcaga ggttaagcta tccccaatca ttaaggcaga agatttatca tgacaattaa
    10141 actttttgag agcgtattta aatatttcag gatttggttt tgatattttc aactcttcag
    10201 atacaaccat tccatcaata tattctttta tttttgaatt ttcaagtctt ttcttttgaa
    10261 ctgcagaaat tccatttgtt attataacaa tagtatactt ttttttaaga tattgtaata
    10321 tatcgattgc acctggaatt aaaaatgtac attcacctaa aaaatttaag tacattttgc
    10381 taagagtttc agaatcaaca tccaaagata ttttatttgc aaaaagttca aatctaagta
    10441 atttaagttc attcaaagtt ataatatttt tttcaaggtc gagccaaagc ttatcactta
    10501 tatttctata tgactctata taattctcaa aattaaattc taaatcaaac tcagatagaa
    10561 gttttttaaa agcatgtgat tcacttttct taaaatcaaa taaagtatca tctgcatcaa
    10621 aaaatataaa tttatatgtc ttcatggttt ttactccttt acaagatgac tgcaaaaatt
    10681 actttacttt cactatttga gtcattttgc catttatgtt ttgttcctaa aggaactttt
    10741 acactatctc ctttggacaa agtaaattca tcatcatcca taaaaagttt aacttctccc
    10801 tccaaaacat atgcaatttc atctccatta tgagtaatcc tatcaacaca agaagaagtg
    10861 tttggagcta aatccataat tgcaaattct aaattaccac tagagtctgg aattaaaatc
    10921 tcatatataa cttctttgct tccaggtaaa ataacttttt ttctattatc atgtcttact
    10981 atcaagtttt ttttatctac ttcagataca aaaaatgtaa ataaaggaac gtttagagaa
    11041 gaggctatag attttaatga atttacagat ggattagcaa gacctcgttc tatttggctc
    11101 aataatgatg gtgtaacatc agctagtttt gctaaatctc gaatacttaa gttttgtttt
    11161 tttcgaactt cagctatttt ttcccctaaa tttaaatcat tcatataaat atcctctctt
    11221 tttaatttaa aaaatattaa cttaaaataa tcaagtaaaa tgattatgta gtatgaaatt
    11281 attataacat atagaattag gttttgaaaa aactatgttc aatataatta aataaattaa
    11341 aatataattg acaaagtgga tacagaatac ttaaaatata tttaaataaa attaagcaaa
    11401 agatagttaa tttatgatag gaggggatat tatgcaaata acgacttttg gagctatatt
    11461 tggtttatta atagcaatta ttttaattat aaaaaaattt caagctgtat atagtttaat
    11521 gttaggagca tttattggag gattagttgg aggagcaaat ataacacaaa ctgtagattt
    11581 tatggcaaat ggagctatga atatatcacc atcaattctt agagctttgg catcaggtgt
    11641 attagctgga agtttaataa aaacaggggc agtagataaa atatcagaac agattgtaaa
    11701 aatatttggt gaaaagaggg ctttattttc gattgccatt tcaactatgg tacttgctgg
    11761 agtaggcgta aatttggatg tatctataat aactgtagca cctataggat tatatatagg
    11821 taggaaatta aattattcta aattgtcaat actattagct atgttgggag ggggtaaagc
    11881 aggtaatata atatcaccaa atccaaatac tatagcagta gcagataatt tttctgtcaa
    11941 tctctctagt gttatgatgg caaatataat tccagcaatt attggagttg ttattacagt
    12001 tattttagca agtatattaa taaataaagg aaacaaagta caatcatatg aaattttaga
    12061 gcaaagagaa gatttaccaa gtttatttaa atcattatgt ggtccaatta tagctatatt
    12121 tctattgttt ttaggaaatg tatctcctat agttatagac ccaatgatag cccttccaat
    12181 aggaggaatc gtaactctaa ttgtcacagg aaacttgaac aattcaagag aatatttagc
    12241 ttttggatta tctaagatgc aaggggtatg catattatta cttggaacag ggactatcgc
    12301 aggaattata cagatgtctg aattacaaca gagtacaata ggtgctttac aatttttaaa
    12361 tatgccacaa tttttattgg caccagtttc gggtatactt atgtcattag caacagcatc
    12421 atcaacagct ggtgctacta tagcatcatc tacttttcat gatgctataa taaatggtgg
    12481 gctatcaccg atttcaggtg catcaatagt aaatgctggg tctagtgtat tcgagcaatt
    12541 accacatggc tcactatttc atactagttc aggtagtata aacatggata taggtgaaag
    12601 atttaagtta attccatatg aagcattgat aggaatagta atgactataa tttcaacatc
    12661 tattcaatta gttttataat aaatataaat atttaatgaa aaaagtagga ttttgttatt
    12721 aaatattgaa tttattatag taaaaaagaa gattggagtt ttaaagtgaa taaaaaaaag
    12781 attgttatta tagggattat ttattcattt ttagtagtat tttcacttac aaatatgtat
    12841 gtaaatatgg agtataatct aaatgcattt gaatatatta agaagtcact tccatttaca
    12901 gaagaagaaa aaaagtggtt agaaaaacac aagaatttga tttacagctc cgaccaaagt
    12961 tcacctcctt tgagatataa aggtaaggaa gatggacagt ataagggaat tattgttgat
    13021 ttaataaatt ctctgtcaat tcaaattggt agagattttt attttaaacc aaataattgg
    13081 tggaaagaat ctttcgtaaa ttcaatagat gatagtataa agttttttga cttgattcca
    13141 tcaaaagaaa gggcaaataa atttatattt actgacccaa tatacacttt gagtgcaaat
    13201 atattgaaag ataaaaagtc ccaagatata aatagttaca tggatttaaa ggggaaaaca
    13261 gtagcaattc cagaaggtga ttattctata aattttttaa agcaaaaaat tcaagatata
    13321 aacattttat tgactccaga tataaagact ggagtcaacc atttaatgtc tggaaaagta
    13381 gacgcagttg ttggtgatga accagtattg cgatactata taaataatta tggactttca
    13441 aataaataca gtgttttaag taatcctata tatactaaaa aagcagttct tgctgtacct
    13501 aaacaatatg aagagctggt tagtatttta aataaaggca tttttaaact tcagaaaaat
    13561 ggagtgtata aagatttaaa gaaaaaatgg tattctacgt ataatgaagt tgatgatata
    13621 ttgtatgagc gaggaattgt gcctagtatt tatcttttta taggtataat tttaatatca
    13681 atttatgttt tttatagcta tacatatttg ttaaagatag aaatcaaaag gaaaacagaa
    13741 caggtaatag aaaataaaaa aactttagag gcaactttta atagtattac agatataata
    13801 atgcttgtag atgaaaataa caatatagta gaatcgaaca aagtactata tgactttatg
    13861 ggtgaaatga gttataagat agcagatttg attagcatga taaaaggtgt tattgaaaat
    13921 accttctctg aaaatactaa taaaactagt gaaatagaaa tacacaataa aattttaaag
    13981 ataaacactt ttcctgtaga gtataaaaaa aataatactg agtatatagt agttcttatt
    14041 aaagatataa ctaatgataa aatagtagag gcgaaattac ttagagaaaa caaaatgata
    14101 tctataggac aattagcttc tggggtagca catgagataa gaaatccttt gggtataata
    14161 agaaataatt gctatctctt aaaagataac gttacaatgg aagaagtaaa tgattgtgtt
    14221 aaatcaatag aaagtaatgt agatagggca agtaatataa taaccaactt gttaaatttc
    14281 gcaagaatat cagatgataa tttagaacat ataaatataa gaaattttat tgaaaatata
    14341 gtgaaacttc aatataaaat gttacagtta aagaatgttg aaataaaaat tgactgtgaa
    14401 cataatttaa tatgctatat aaatggagaa tcgttaaaac atgtatttat aaatttaatt
    14461 tctaattcaa tagatgcaat acatcaagat ggaaaaatta ttatatactg ttatgaaaaa
    14521 aatcattgtt tatttataga ttttaaagat aatggtgaag gaataaaaga agatgcccta
    14581 aaggacatat ttaatccctt ctatacaaca aaaccaattg gtgagggaac tggtttaggt
    14641 ttatacatta cttataatga gattaaaaag aataatggag atataagtgt tgaaagtaaa
    14701 ttgggggttg gaacttgttt tcatataaaa attccattaa ataaagaggt gactatataa
    14761 tgaaaatact attagttgat gatgagctag aatatggagt ggtaatgaaa aaaattctac
    14821 aaaagaaggg ctatcttgta gatatagcac taagtggtga agaagctata aatataatta
    14881 aaaaagataa aaattatgat ttggttttat ctgatgttat gatgaaaaat atggatggag
    14941 tacagctatt agatagaatt aaatctatta atagagatat agaggttata ttagttacag
    15001 gatatggaag tattgaaaat gcagttgatg ctatgaaaag aggagcttta tcttatttta
    15061 taaaaagtaa tcccattgaa aatttattag aagaagttga aaaggtaaag gcaagtaaaa
    15121 tatctgtttc tgtacaaaaa aataatctgg aatttacttt ggaatctaaa aatagagatt
    15181 ttaataatat cataaaaata gcaaaaaaag ctgcttgtaa agatgttaat atattaatct
    15241 tgggggaatc tggtgtagga aaagatatat tagctagata tatacatagc ataagtccaa
    15301 gaaagaatga gatatttgta cctgtaaatt gttgttcttt ttcagaaaat ctattggagt
    15361 ctgagttgtt tggtcatgaa aaaggttctt ttacaggagc agtagatagt agaaaaggta
    15421 gatttgaact ttcgaataaa ggaactctat ttttggatga aataggagat atacctctaa
    15481 atgttcaggt aaaacttttg aggacacttg aggacaaatc tatagagagg ataggaagta
    15541 ataaatctat taaagttgac tttaggttaa tttgtgctat gaataaagaa ccaaaagtgg
    15601 aaatatcaaa tggaaatata agagaagatt ttttttatag aataagtaca ataacaataa
    15661 ctataccacc actaagaaag agaagagaag acttaactac tcttatagaa tttttcttaa
    15721 ataagtacca aattgaacat gataaaaaga tacactcgat tgataaggaa gtaaaggatt
    15781 ttttattaaa ttacaattat ccaggaaata taagagaatt aaaaaatata ataaatagac
    15841 ttgtagtttt aagtgaagaa ggcaatttat caaaagacaa tttaaattta atatcaaata
    15901 atgtatacat agatgataaa ataagtatta gacctttaag agaaataaga aaagagtttg
    15961 aatgtgagta tattgaaaaa gttttatctt tgtgtggaaa taatatttca aatacagcta
    16021 aaaaattgga gattagtaga agacagttaa ccaataaaat atcagagtat aatattaagt
    16081 gagaaaaaaa cttctcatta agtgagaaaa aaatttctca aactgtatta aaagataaac
    16141 agaaaaaatc tgtttatctt tttttattat aattttaaaa attaatattt acaataatta
    16201 ttggtaaatt caatattggt gtaaa
//
LOCUS       DAEMHZ010000093        16079 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000093 DAEMHZ010000000
VERSION     DAEMHZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.093"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            945..2615
                     /locus_tag="KPK72_002642"
     CDS             945..2615
                     /locus_tag="KPK72_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG2489843.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2737..3471)
                     /gene="pflA"
                     /locus_tag="KPK72_002643"
     CDS             complement(2737..3471)
                     /gene="pflA"
                     /locus_tag="KPK72_002643"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG2489844.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWENLGIDYELKD
                     VPDATKEDIQKASEIISKFGVKVYNS"
     gene            complement(3631..5862)
                     /gene="pflB"
                     /locus_tag="KPK72_002644"
     CDS             complement(3631..5862)
                     /gene="pflB"
                     /locus_tag="KPK72_002644"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG2489845.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6155..7216
                     /locus_tag="KPK72_002645"
     CDS             6155..7216
                     /locus_tag="KPK72_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG2489846.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSIIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7307..8920
                     /locus_tag="KPK72_002646"
     CDS             7307..8920
                     /locus_tag="KPK72_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2489847.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9195..11180
                     /locus_tag="KPK72_002647"
     CDS             9195..11180
                     /locus_tag="KPK72_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2489848.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            11185..11619
                     /locus_tag="KPK72_002648"
     CDS             11185..11619
                     /locus_tag="KPK72_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG2489849.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11648..12208
                     /gene="srlA"
                     /locus_tag="KPK72_002649"
     CDS             11648..12208
                     /gene="srlA"
                     /locus_tag="KPK72_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2489850.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12272..12640
                     /locus_tag="KPK72_002650"
     CDS             12272..12640
                     /locus_tag="KPK72_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG2489851.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12665..13231
                     /locus_tag="KPK72_002651"
     CDS             12665..13231
                     /locus_tag="KPK72_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2489852.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13272..13637
                     /locus_tag="KPK72_002652"
     CDS             13272..13637
                     /locus_tag="KPK72_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2489853.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13738..14511
                     /gene="srlD"
                     /locus_tag="KPK72_002653"
     CDS             13738..14511
                     /gene="srlD"
                     /locus_tag="KPK72_002653"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG2489854.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14860..>16079
                     /locus_tag="KPK72_002654"
     CDS             14860..>16079
                     /locus_tag="KPK72_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2489855.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     ITSNKVNCVSDELD"
BASE COUNT         6219 a         1848 c         2711 g         5301 t
ORIGIN      
        1 cctaacataa aaacaaaaaa tgttcataaa tttaactatt aacatagata atttttagaa
       61 aaatatctaa attttcaata aatagtaact tttttataac aaatggaaac tatggatgta
      121 ataattaaaa taaaaaatat tatttttatt tataaacatt gacattctaa ttttgtagtg
      181 taatcgtatt ttttattaat tcttacaaaa tcaatgaaaa taagtatatc cattttaaag
      241 tacataaatt taaaaatcca gaaaaatata cttaaattat gtaagtaaaa gaggggttga
      301 atcagttgaa aatgtaatat tagtaaatag tacatcaatc ctatataccc tgtaagcaac
      361 tctattttca tgtactaaaa atgtacctat tttattaact gaaaagaata aattaaatgt
      421 aagtacataa gaagatagaa aaaattaaaa acaaaaaata tttatataaa taaatataaa
      481 aaatgatata atccctatta aaaaatttat agatgatgaa gaaatatcaa aatcttatat
      541 tataggaaga gacacttctt tgccaaaatc tatagaaaag caataccaaa tccaaagaga
      601 ataggcgaag aaaaaaataa aataaattca agagtaatag aaaagtttta tagaataaag
      661 aactgataac tctttatata acgaaaaata gaattttaag agaaaatgat ttaataaatg
      721 atttaacatg gtgttttagc aggtaaaaat aaatcaccag taatgattat agaaaaatta
      781 ctttatgaat ctcaaaaaac ttagttaaga gtaagagatt tgataagata actaaagttg
      841 gggaaatgtg tttgatgaga taaaaaatta gtaaaatagt ataatctaaa taaggatatt
      901 ttgttgatta aactacacta atagtaatat tgtaggggga aaagatgaga gaagaatcga
      961 actctttaag tttagaccat atagaaattt tctttgaact tttatctcaa agtacagaag
     1021 attatatatt tttttgggat ataaatagga ataaatttaa aatttcttca gcaatttttg
     1081 atgaatttaa cttaagtaaa gagatagaat ctgatttggt caattgttgg agtaaaattg
     1141 tttatccgga tgatgttcaa atatggaaag atgatataca agaattacta catggaaaaa
     1201 aaggtgagca caatttagaa tataggctga ttaataaata tgaagaaata gtatggattt
     1261 cttgtagagg taaagtttat gtgtcggatg acccaaaaac tatatttcta gtcgggagaa
     1321 taaagaatat tggtgaaaaa aataaatttg attctatcac aggaacgtgg aatagagaac
     1381 aatttgaaca tagaatgaat tatcttataa aagagaaaat ttataaaaat ggtgctatgt
     1441 ttattatgga tattgataat tttaaaaata taaatgaaaa atatggacat tcttatggtg
     1501 acaaagtatt gcgtgcaatt gctactgaag tattagaata tcttccaaaa gatgtaagat
     1561 tgtataggtt agatggagat gaatttgcat ttttttaccc tatgtgtact aaagaaacta
     1621 tagagaaaat atatgaaaag atacagatgt atacaaatac tcagcatgaa attgaatcaa
     1681 ataagtacta ttgcacagtg actgcaggcg ttgctatgta tccagaagat ggagataatt
     1741 atttagattt atttaaacat gctgatattg cattggatat agcaaaaatt agtggtaaaa
     1801 atagaattaa gtttttttca caagagctct atgaaaataa actaaaggta atttcaatgc
     1861 aacaaaaact aagagagtgc gttgaaaata attttaatga ttttgagctg ttttttcaac
     1921 cccaggtaaa tgctgttaca aaagaggtaa taggggcaga ggtactttta agatggcatt
     1981 cgagtactta tggagaagta tctcctgttg aatttatacc aatactagaa cagagtaatt
     2041 taattatacc agttgggaag tggattataa aagaagctgt aaaacagtgt aaagaatggc
     2101 ataaaataaa tccagacttt aaaatatctg taaatgtatc gtatattcag ttaaaggaag
     2161 atttttttag agattttata gtagaatgtt tagtagagta tcaattaaga cctgaatttt
     2221 taattttgga attaacagag aattgttgga taccagatat aaatttacta aatgataaat
     2281 ttattagttt aaagggtata ggagtatata ttgcaataga tgactttggt acaggatatt
     2341 catctttaaa ttaccttaaa gaactgtctg ttaatataat aaaaattgaa agaagttttg
     2401 ttaagaatat tacatataat agttatgaat atacttttct tgaatatatt attaaattag
     2461 ctcatattat aaatttaaaa gtttgtgttg agggaataga gtcttatgaa gagtatgata
     2521 tagtaaaaag cttaggtgta gatattattc agggtttttt atttggaaga cctgtaagtg
     2581 cctctgaatt ttataaattt caactatcaa agtgatgtat ataaaatgag atatatagta
     2641 tataaaaata gggcaaagtg gaagttttga agtttattaa aaatttacac tttaccctat
     2701 tttgttatgt cttctataaa ggattaatat actacactaa ctattatata ctttcactcc
     2761 aaatttagag attatttcac tagctttttg gatatcttct ttagtggcat caggaacatc
     2821 ttttagttca taatcgatac ctaaattttc ccatttgtgt attcccatag aatggtatgg
     2881 tagtaattca aatctttcta catttttcaa tgtagctaca aattgaccta gtttttctaa
     2941 attatctata tcatcagtaa tgccaggaac taaaacatgt cttatccata caggaatatt
     3001 tcttgattct aagtgtctag caagttttaa tgccttgtcc ataccaacac cagtcaaatc
     3061 aatagatttt tcctcaacca tatgttttaa atcaagtaaa accaaatcag tattatctaa
     3121 aactgggtct atagtttcta tatctacaaa tccagatgta tccaagcaag tgtgtatatc
     3181 attttctttt gcttttttaa ataattcact taaaaattct ggttgaagag tggattcacc
     3241 accagaggca gttatacctc caccagaaaa cttcataaaa gaagtatact taagagcatc
     3301 cgttattatt tcatcaactg tgtattcttt tcctgattta atatcccatg tatccctgtt
     3361 atgacagtat ttgcatctaa gaggacaccc ctgaaaaaat aatatatatc ttattcctgg
     3421 tccatctaca gtaccaaacg tttctataga atgtatcttt cccttaatca tagagttcac
     3481 ctcacaatat attttaataa ccttatcaat agttataata tttctctaaa aaaggccatc
     3541 ttataccaga aataattcaa atataagata gccttttatt tttatttttt acacagtagt
     3601 aatcttagtt aaagtataaa aatttaaaga ctaagccatt ttaccgtgga atgttctgtt
     3661 tataacatct aattgttgct ctttagttaa ttttatgaag ttaactgcat atccagatac
     3721 tcttatagtt aattgtggat attcttctgg atgttccata gcatcttcta aagtagctct
     3781 atcaaataca tttacattta agtgatgagc attttgagcg aagtatccat ccatcattgc
     3841 agaaagattg tttattcttt cagtcatatc ttttcctaaa gcagcaggta ctatagagaa
     3901 agtattagat ataccatctt gagcatgttc atatggtaat ttagcaactg aagataatga
     3961 agctaaagca ccattagtat ctctaccatg cattgggtta gcaccaggag caaatggagc
     4021 accagctctt ctaccatctg gtgtattacc agttttctta ccataaacaa catttgaagt
     4081 tattgtaagt atagattgag taggataaga atttctataa gttttattct ttcttatttt
     4141 attcataaag ctttcaacta actctacagc tatactatca actctatcat cattatttcc
     4201 gtattttgga taatctcctt ctatttcaaa atcaacagct attccttgtt catttctaat
     4261 agttttaacc ttagcatgtt ttatagcaga taatgagtct gcacaaactg ataacccagc
     4321 cataccacaa gccatagttc taaatatatc tctatcatgt aatgccattt ctaatgcttc
     4381 ataagaatat ttatcatgca tataatgtat tacatttaaa gtatttacat ataatgttgc
     4441 taaccaatca gtgaatggtt caaatttact cataacttca tcataatcta agtactcaga
     4501 agttataggt tcaaatcttg gtccaacttg aacaccagat ttctcatcta caccaccatt
     4561 tatagcgtat aataaagttt tagctaagtt aactctagct ccaaagaact gcatttgttt
     4621 accaattctc attgcagata cacaacaagc tataccatag tcatctcccc agtatgctct
     4681 cattaaatca tcattttcat actgtactga acttgtatct atagatactt tagaacagaa
     4741 atctttgaat ccttgaggta atttagttga ccatagaact gttaagtttg gttctggaga
     4801 aggtcctaat gtatatagag tatttaacat tctaaatgag tttttagtaa ctaatgttct
     4861 accatctatt cccatacctc ctatacactc agttacccaa gttgggtctc cagagaatag
     4921 gttattgtaa tcaggagttc ttaggaactt aaccattctt aatttcataa caaaatggtc
     4981 catatactct tgtaattctt cttctgttac gattccagct tttaaatctc tttcaaagta
     5041 tatatctaag aaagtagatg ttctacctaa agacattgca gcaccatttt ggtctttaac
     5101 agcacctaaa taaccaaagt ataaccattg tacagcttct tttgagttag ttgctggttt
     5161 tgatatatca aatccataag attcagccat ttttttaagt tcatttaaag ctgatatttg
     5221 ctcagttatt tcttctctta atctgataac ttcctcatcc atgcaagaaa cttctaatga
     5281 tttcttttgc tcatttttat cttctattaa agcatcaaca ccgtatagag caactcttct
     5341 gtagtcacca attattctac ctctaccata agcatctgga agacctgtta ttataccaga
     5401 ttttctagca gctctcattt ctggagtata aacatcaaaa actccttggt tgtgtgtttt
     5461 tctgtattta gtaaatatat cttttatttc tgggtctaat tcatatccaa aagcttcaca
     5521 tgaattttca accatcttta taccaccata tggcatgaca gctcttttta aaggggcatc
     5581 cgtttgtaac ccaactattg tttctaattc tttatctaaa tatcctggag catatgcagc
     5641 tattccagaa acagtttttg tatcaacatc taaagtacca ccattttctc tttctttttt
     5701 aaataggacc attgcttcgt cccataattt tttagtattt tcagtggcac cagctaagaa
     5761 agagtcattt ccctcatatg gagaatagtt taattgtata aactctctaa catttatttc
     5821 tttagtccat cttcctgttt taaatccttg ccatgcattc attaagtatt tcctccttag
     5881 atttaaatga taaataatac tattaaaatt aatttttata tacttaattt attttatata
     5941 tttttttaaa aatatatttt ctaatataat aatagcatat tattcgtatc ctgtatacat
     6001 aaaaaataaa aaattgtcgc tttaaagtat aaaacagatt atatgttaat aatgtggtga
     6061 aattataccc atatataaat aaaataaaac aaataaatag ctaattaagg agaaaatatt
     6121 ttttattttt tgtttaggat aaataaattt tattatggaa aaaagaaaaa gcatacatct
     6181 tcacaaactg aatatttatt taataggggg aggtgataga ttgtttatca cagtggtatt
     6241 atttttagta ggtttcctat taattactaa aggcgcagat atctttataa attgtactgt
     6301 agagataggt aaaaaaacga atatatcaga aattatactt ggagctacta tagttagttt
     6361 tgcaacaaca ttacctgagt ttacagtgtc tctacttgct tctattgatg gtcatactac
     6421 tatgagttta ggaaatgcag ttggttctat aatatgtaat acagggttag cattagggtt
     6481 ggttgttttt ataagtccat ttaatgtaga taaaaaaatg tttttttcta agtctttact
     6541 attaatagta tccattatag ttttaatttt attgagttta gatggagtta taacaagagg
     6601 tgattcatta ttacttataa taatattaat cttttatatg gttaataatt atagaagtgt
     6661 cgttggaaaa tctgatacta aaaatagaaa tattaaaaat aacactatta aagacatatc
     6721 tactaaaaaa gggaaaaatt atagaggttt ttccgtatta gaaattgtaa aaatactatt
     6781 actttttgct acaggattga taatgatgat aataggttca caaatcctta tagagagtgg
     6841 agtaataata gctagttttt taaatatacc tcaaggtata gtaagtttaa ctataatagc
     6901 acttggaact tctctgccag agatagtttc gtcgattact gctataagaa aaaatcatca
     6961 tgaaatatca gtaggaaata ttttgggtgc aaacatattg aatatagtat cagtaatagc
     7021 tgtatcagca ataccaaata acataccaat actatctcaa aatagacaat tggatatacc
     7081 atttatgata ttattattat taatagtaat aataccaaca cttaaatcta ataaacttag
     7141 tagaatacaa ggtatattga tgttattcac atattttctg tacatatcaa ttttgtattt
     7201 catgtatatt atttaactat actgtttaaa atattaattg cagaatatcc taaaaaagta
     7261 tataatggat aggttaagat taattgataa cggagaggaa taatatatga atataacaaa
     7321 gtttgaagat ttaccaataa gtgagggaat aaaaaaagct atagctgaaa tggggtttga
     7381 agaaccatct cctatacaag cacagtctat acctgcgatt ttgtcaggta aagacgttat
     7441 aggacaagct caaactggta cgggaaaaac agctgctttt agtatcccta tactagaaac
     7501 aatagaccca aataatagaa gtttacaagc tgtagtgctt tgtccaacaa gagaactggc
     7561 aatacaagta tcaacagaaa taagaaagtt agctaagtat tcgcacggaa taaagacatt
     7621 acctatatat ggtggtcagc caatagatag acaaataaaa tctctaaaga gtggtgtgca
     7681 agttgtaata ggtacaccag gacgtacaat agaccatatc aatcgtaaaa cattaaagat
     7741 ggataatgta aaaatgatta tattagatga agcagatgaa atgctagata tgggatttag
     7801 agaagatata gaaatgattt taagtaagat tccagaagaa agacaaacaa ctttcttttc
     7861 agctactatg ccaagaggaa ttttggaatt aactaagaga tatcaaaaag acccagagca
     7921 tataaaagtt gttagaaaag aacttacagt ttcaaataca aagcaatact atatagaaac
     7981 taggtcatca aataaattag aagtattatg tagattagta gatgtatatg accctaaact
     8041 atctgttgtt ttctgtaata ctaagagaaa agcagacgaa ttggtaggag atttacaagc
     8101 aagaggatat tttgcagatg cattacatgg tgacttaaaa cagactcaaa gagatatagt
     8161 aatggataag tttagaaatg gtacaataga tatacttgtt gctactgatg ttgcagctag
     8221 aggtatagat gttgatgatg ttgaatgtgt attcaactat gacttaccac aagatgaaga
     8281 gtattacgtt catagaattg gtagaactgg tcgtgctggt agagaaggta tgtcatttac
     8341 ttttgtattt ggaaaagaaa tgagaaagat gaaagatata gaaagatata ctaaatctaa
     8401 actaataaag cataatatac caacaataac tgatgttgaa gagaaaaaag taggtacttt
     8461 ctttgcacaa gttaaacaaa ctatagaaga aggtcactta actaaacaat tacaatggtt
     8521 agaaggtttc tgtaatgatg aagattatgc aatggttgat atagctgctg cacttgtaaa
     8581 actatctcta ggtgaagaaa tgaaagaaga aataatagaa gaaaaaccta gaagagagcg
     8641 tggagataga aaaggtggta ctggagctaa agatggtatg attagattat ttatcaatat
     8701 tggtagaaat caaagagtac aagcaaaaga tatagttggt gccatagctg gagaagttgg
     8761 aataccaggt aaagtagtag gtactataga tatatatgat aagtatactt ttgttgaaat
     8821 acctaaaaaa gatgcaaaaa cagtaattga aaagatgaaa gatattaaga taaaaggtaa
     8881 taagattaat atagaaaaag caaataagaa gaaaaaataa cgtatagtat acaaaactat
     8941 aattagaaaa tatataaaat aatatattgg atatttaaaa agcctgatgt gttttcaaaa
     9001 cagatagtag ataatctgta agagttaaca gcataaggct tttcttgttt tatatgatgt
     9061 tataattttc cagattgttg tgaaaaatgt atattagttc ctttttttca aatctgattt
     9121 gaaaaagaga atattaatta tctatatagt ttagattata ataaaaaatg taagataaca
     9181 aaggggagaa aaagatgtca atgacttata tggaccctag ggtttttttg attttagaaa
     9241 tggtgttaga agaagacaca aatactctag aacaattttc aaatgttttg ggtgttagta
     9301 ctaggactat cagaaactat ataaaccaga ttaatcgaga attaaaaggt ggtattgcta
     9361 aaatagttaa aaatgaaaaa ggtacatata gtataagaat tgaggatgaa agtaaattaa
     9421 ttgagattgt aaattttaat agaggaaagc tatcaaattt tataaattta aattcacctg
     9481 atgaaagaat aaattatgtt cttgatattt taactatgac caacaatcca attaccatag
     9541 atgatttggc tgaagagata tgtataggaa gaacaacatt aataaaggat ttaaaaaaag
     9601 cagacagaat tttagctgaa tatgatttag aattaaaaag gaagccaaat ataggtatga
     9661 ttttagttgg tgaagaaatg gatataagat tattaatcct tgatagattg tatgaaaatt
     9721 atattgatgt cttggaaaac gttaatcaga taaatttagt taaagattat gatattgaat
     9781 gtcttagaga tgaattaaaa aagttattta aaaaagaaga actctacata acagaacagg
     9841 tcttgagaga tgttgaaaga tatataatca tgtctgtctt aagaaatcta aatgggtaca
     9901 aatttgaaaa gatagataag agatttgaag tgataagatg ttcagatgaa tatactttag
     9961 gattaaagct caaagcttta ttggaaaaga tatttaatat agttttaaat gaaaaagaaa
    10021 ccatattttt gacaatgcca ttaataggta gaaaagctcc tagtacaaaa ttagcactta
    10081 gcagtataaa gataaatgat agcgtaaagg aagttgtaga tgaagtaact agttttttat
    10141 tagagacctc agggattgat tttaaagaag ataaaaagct tatagaaaat ttggaatatc
    10201 acttgtattt tgcacttaat agaatgagat tcaacataag ggtaaaaaat cctcttttac
    10261 aagaaataaa ggaaagatat acatttccat atagtgttgc aaagatagct gcaatgataa
    10321 ttgaggaaaa gtttgacttg aaaatatgtg accacgaaat aggatatata gcattacatt
    10381 ttggaagtta ttttgaaaaa aattcaagaa aagttctaaa tgtaaatagg gtagctgttg
    10441 tttgtggaac aggtcttggg actgcacaac ttattagaat aaagatagga aaaatacttg
    10501 gagaaagtgt agaagtaaat actttttctg atatagaagc taaagctaat ttagatttat
    10561 taaagtatta tgatttagta tttacaacag tagatttcaa aacagactta gatgttccaa
    10621 tttttagagt aaatgcgtta tttaatgaag aaagtataaa gaaagagata gaaaatgcaa
    10681 tacttttgaa aaatagtata aatcttaagt ctataaaaaa gattgaaaaa aatatccctt
    10741 ttataggtaa attaattgaa gaggataagt tctttatatt gaataagagt acgtttatgg
    10801 aaaatttaga agatatgtta gaaaaattag ttcataaagg aattattgat atagagttca
    10861 aaaaaaaggt attaaaaaga gaaaagaaat cttcaacagc attagataat tatatagcat
    10921 tgcctcactc agttaatact aatggggaaa atctttatct ggcatttgga atattagaaa
    10981 aaccagtgat ttgggataca aaggaaatta gaataataat attgatgata attccagata
    11041 agaatgtaga tagtacagac ctgataataa aaagttatga agaagtactt gaagtaggga
    11101 gagataaaaa gatggtagaa gaacttgcta aggtaaaatc atttgatgaa ttttataaaa
    11161 taattaccaa gaggaggtaa taatatggac ccagtaattt ttattatagc atcacttatt
    11221 acagtgtatt tattaaatat ttttcttgga tacttacagc ttaaagattt taataaaaat
    11281 tatattgaac taaaaagaaa aggaagagtg gcaattggca gaaaaaaagg aagaatttca
    11341 tcgggtacaa ttgtgttgat acttataaat gatgatggtg tgattgtcga gactagaaaa
    11401 atgcaaggtg ttacagtatt ggcaagagta aagtcatttg aaggtttggt aggaaagagc
    11461 ttagagtata tagaaaaatc agatttaact gtttacaata aactacttaa aaaagcaata
    11521 ctagatgcag tcaaacagta cataacattt gagaaaaata aatgtgaaga tacaaataat
    11581 ctgagtactg atgaagagtg taggatggaa gtagtttaaa aaaatatatt tataaggagg
    11641 agttaatatg acgcaatttt ttcaagtatt agcaagtggt gcagatagtt ttatgaattt
    11701 atttagggca ggtgggcaac aatttgtagg ttttgtaaca gatatagtac cacttttagt
    11761 agcattatta gttacaatga atgcaattat caactttatt ggtacagata gagtcgagag
    11821 acttgcaaaa aaatgttctg caaatatatt tactagatat ttgatattac cagtccttgg
    11881 tacatttgta tttgcaaacc caatgacact atcattagga agattcttgc cagaaaagtt
    11941 taaacctagt tactttgcag cagcaagctt ttcatgccat acaatgaatg gattattccc
    12001 acatatcaat ccaggtgaac tatttatata tctaggaatt gcaaatggta taacaacttt
    12061 gggattctca acagcagatt tagcagttag atatttgtta gtgggtatag ttgcaaactt
    12121 tattaaaggt gtagttactg actatacaac aaaatttgta gagaaacaac aaggaatagc
    12181 attaaattca gatataaaga ttggataatt actttaaggt ataaataaat ttttcaaata
    12241 aaaagatagt gtatagagga ggataaatta tatggcattt aacaaagtta aaatagtaaa
    12301 aggttctgga ggatggggtg gaccattgat aatagaacca acagaaaaga agaataaggt
    12361 agtatatgtg actgggggag caaagccaga aactgcagta aaaatagctg aacttacagg
    12421 atgtgagtta atagatggat tttcacaggg tgttaaagat gatgaaatcg catgtgtaat
    12481 aataaattgt ggtggaacat tgagatgtgg tatatatcct caaaagaaaa taccgactat
    12541 aaatataatg aaaacaggaa aaagtggacc attagcaatg tttataaaag aagatatata
    12601 tgtttcaggg gtaaaaccaa caaatgtaga gcagttataa ttgaggtagt aggagggaag
    12661 aaatatggct tatgatacta aaaagaagat aagtgagcag agtcaaggaa ttgttgcaaa
    12721 aataggtata gcactaggaa atgtgacaag tactcttttt caagctggta gggatactat
    12781 agatacatta atacatacta tactgccttt tatggctttc gttgcagtcc taataggtat
    12841 aataaatcaa tcgggtatag gaaattgggt agccaattta cttactccat taatcggaaa
    12901 tgttggaggt ttagttgtct tatcaataat atgttcattt ccgttgttgt ctccgtttct
    12961 tggaccagga gctgttatag ctcagatagt gggggtattg gttggtacag aaataggaaa
    13021 aggaaatatt ccaccaagtt tagcattacc agcattgttt gcaataaact cacaagcagc
    13081 ttgtgacttt ataccagtag gtcttggtct tgcagaagct gaacctgata cagttcaagt
    13141 aggagttccg tcagttttat attctagatt tttaacaggt gcaccaactg ttctattagc
    13201 atggttagca agctttgggt tatataaata gaagaatact ttataaataa ttagagagag
    13261 gttcagttga tatggaatta atatacagta ctcaaataaa taaaatagga gataatgcaa
    13321 atgaattttt acaacataat atgtttgtaa ctttcaaaga taatgctcct caagagttag
    13381 aaaattactg ttatatacat tctgaaaata atttagtaaa agaaatactt acggatgatg
    13441 tgctatcaat aaataatgtt gattacaaaa ttacttctgt tggtgagtta gtaaaccaaa
    13501 atctttcaga attaggtcat ataacattta agtttacagg tgaaaaagat ggagcaattg
    13561 gaggaacctt atatcttgaa gaaaaagaaa tagcacctat tggcgtaggt actatactaa
    13621 aaataattag aaaataaaat ttaaaacgaa tctaatcata ttataagtaa attatagata
    13681 aaagtcaatt taactaatca agttaacttt taatgtgata aaaaagggga aatgaaaatg
    13741 agaaaagtgg ctttagtcgt tggaggagga agaagtttag gtgaatattt atctaaacat
    13801 ttagctaaag taggttatga tgttgctatt gcagatttaa atgaagacaa cgcaaaaaaa
    13861 gtagctgaag aaataaaaac tgaatttggt tgtaaatctg taggatatag ttgtaatgcc
    13921 atagatgaaa gacaggttat aagtactgtc aaagctattg aaaatgattt tgatagaata
    13981 gatttattag tgtacaatgc aggagtggct gtaagtaaaa aaataactga ttttggatac
    14041 aaagatttta aatttattgt agacgtaaat ttaaatgggt actttctatt tgcaagagaa
    14101 gtatctaaag tcatgatagc aaaaggtatt gagggtagaa taatacagat aaattcaaaa
    14161 tcaggtagag tgggaagcaa gcataattca ggatattcag caggtaaatt tgctggagta
    14221 ggacttactc aaagtttagc gctagatttg gcagaacata agataactgt aaatgcactg
    14281 atgctcggta atttacttga ttctgatatg tttgaaagtt taattcctca atatgcagta
    14341 aaattgggaa tacctgaatc agaagtcaag caagtttata tagataaagt acctttaaaa
    14401 agagggtgta gatttataga tgttgcaaat atgcttacat tttatgcttc aaaggaagct
    14461 gaatactgca ctggtcaatc tataaatatt actggtggac aagttatgta aggtttttat
    14521 ggagtgtctc aagatgaact ctttagttca tgaggcactt ttttgtataa aattaaatat
    14581 attttcagca tttaaacaat gaaaaagagg tttatcttta ttttgagata gcctcatttt
    14641 tgtaattaaa aaacatagtg tttaagtaaa catataagat tgttagtttt ttaaaagaga
    14701 attttttaat aatattatat ttatgaagta tttttaaact tattttgata aaatatagct
    14761 atgttttata tactgtagtt ttgaacatag aaagtttaat aaattatagt atatatgtta
    14821 atttttaata aggtaagaaa gtagaaagaa ggatatttta tggatataat aaaaaaatta
    14881 agtcatgggt gtacattaga ttgtcatgac tgttgtaaat tcaatgttta tactaaagga
    14941 aataatgtag taaaaattga aggagataaa aatcatcctt atacaaaagg ttttatatgc
    15001 aaaaaaggga tggcccattt agatagatta aatcataaag atagaataaa gactcctatg
    15061 cttaaagttg atggagtatg gaaagagatt tcttttgata aagctataga aataatggca
    15121 gaaaagctta catactataa agaaaagtat acatcaaagt ctgtcatgca ttatgaccaa
    15181 tatggaagtg ggtctgtgct aaaatacata ggagatatat tttttaattt ttatggaggt
    15241 gttagtagac ataagggggg gccctgttgg agtgcaggga tgcatgctca aaaatatgat
    15301 tttggagtag ctaagagtca tgctatagaa gatatgttaa atagtaaaag tatatttgtt
    15361 tggggaaaaa atccagcata tactacaatt catactatgc aaataattaa aaaagctaaa
    15421 gaaaaaggga taaagatagt agtgattgac cccatatata caaaaacagc tcaaatagca
    15481 gataagtatg tacaagtaaa tcctggaaca gatggagctt tagctatagc tatggcaaaa
    15541 attatagtag aagataaatt atatgatgaa gaatatataa actcgtatgt tattggtttt
    15601 gaagaatata aaaaatattt atcttcattg gaattaagtt ttttaattga tgaatgtggt
    15661 gttaaagaga atgatataag agagttagtg gatttatata ctaacaaata ttcaagtata
    15721 aatgttggat atgggcttca aaaatataaa aatggtggaa acacaataag agcaatagat
    15781 gctttaggtg ctataactgg tcaaatagga tttagtggtg gaggggtaaa ctatgctaat
    15841 aaagtttatc cgagtgttat aaattctgac ccttacaata gccaatcata tggtgaagat
    15901 agggagtttt atgtaagtaa tatcagtaaa tttatagaag aatctttaaa aaatacatca
    15961 aataaggtaa attatgcatc agatgaactt gatatggcat tacataaagt aaattacata
    16021 tcagatgaac ttgatataac atcaaataaa gtaaattgtg tatcagatga acttgatat
//
LOCUS       DAEMHZ010000094        16076 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000094 DAEMHZ010000000
VERSION     DAEMHZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.094"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(77..280)
                     /locus_tag="KPK72_002655"
     CDS             complement(77..280)
                     /locus_tag="KPK72_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2489856.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            571..1335
                     /locus_tag="KPK72_002656"
     CDS             571..1335
                     /locus_tag="KPK72_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2489857.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGYEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            1724..3037
                     /gene="prdC"
                     /locus_tag="KPK72_002657"
     CDS             1724..3037
                     /gene="prdC"
                     /locus_tag="KPK72_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG2489858.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      3440..3525
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            4107..6323
                     /locus_tag="KPK72_002658"
     CDS             4107..6323
                     /locus_tag="KPK72_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG2489859.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQIPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERL
                     KQYQGSIDLCDKLIERAKTEEERAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVE
                     ISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPS
                     PPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNP
                     PVPPVSDTVEKTPELSRDDTIVKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            6730..8490
                     /gene="prdR"
                     /locus_tag="KPK72_002659"
     CDS             6730..8490
                     /gene="prdR"
                     /locus_tag="KPK72_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG2489860.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            8919..10799
                     /gene="prdA"
                     /locus_tag="KPK72_002660"
     CDS             8919..10799
                     /gene="prdA"
                     /locus_tag="KPK72_002660"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG2489861.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            10880..11164
                     /locus_tag="KPK72_002661"
     CDS             10880..11164
                     /locus_tag="KPK72_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489862.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            11202..11927
                     /gene="prdB"
                     /locus_tag="KPK72_002662"
     CDS             11202..11927
                     /gene="prdB"
                     /locus_tag="KPK72_002662"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:11652..11654,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG2489863.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            12007..12765
                     /gene="prdD"
                     /locus_tag="KPK72_002663"
     CDS             12007..12765
                     /gene="prdD"
                     /locus_tag="KPK72_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG2489864.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            12780..13247
                     /locus_tag="KPK72_002664"
     CDS             12780..13247
                     /locus_tag="KPK72_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2489865.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            13265..13747
                     /locus_tag="KPK72_002665"
     CDS             13265..13747
                     /locus_tag="KPK72_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2489866.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            13767..14774
                     /locus_tag="KPK72_002666"
     CDS             13767..14774
                     /locus_tag="KPK72_002666"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG2489867.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            14894..15775
                     /locus_tag="KPK72_002667"
     CDS             14894..15775
                     /locus_tag="KPK72_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2489868.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         6384 a         1940 c         3086 g         4666 t
ORIGIN      
        1 attttataaa taaatataga aaaacagggc tgctaattag cagccctgtt gatacccagg
       61 tgtcaaaatt tactatttat tttccagcca tttgtctttc agccatttca actaatttct
      121 tagtcatata tccaccaaca tatccatttt ctctagctgt taagttacct ttgtctatgt
      181 tttcatagtt agataaacct atttcgttag ctatttctaa tttcatttgg tttaaagcag
      241 cttttgcttc tggaactact gttctgtttg aatttgacat agtaaatttc ctcctcaaat
      301 aattagttta tgatgtctaa caacactttt atttatgtga tgttaatact aatttctcca
      361 aaataaaaat aatatataca gaaaaataaa ggtaaaaatg taaaaaatat aaaaaaatta
      421 aataaatgag tattttagag agtttaatta ggtcaatata ggttttattt ttttagttat
      481 tgtcttataa attaaatatt acagtacttt tttgagctga aaaaaaataa attttaaata
      541 tagaagaaat tatttcaaag ggggaatagt atgaaaagag aagttaaaag gactaaagct
      601 ttgatatata gtgttttatt tttttcatta ttattagtgt taatatttgt ttttgtttat
      661 ggaataaaat acatatgttc taaatctgac aattcaaaaa tgcctatata taaagttgat
      721 acaaatgaga aaaaaatagc tttgagtttt gatgtagcat ggggaactga taatatggat
      781 gatattttaa aaattttaga taaacataat gtaaaagcta ccttcttttt agttggtagt
      841 tgggtggatg ataatgaaga aattgtaaaa gctatagatg aaaaaggata tgaaattgga
      901 aatcattcaa atactcatgc caatttgaag gaaatatcaa aagaagacat aacaaaagag
      961 atagaaacta catcagaaaa aatatataat ataacaggaa aaaggactaa tttatttaga
     1021 cctccttttg gggacgtaaa caataaagct atggacattt gtagtgattt aggttataaa
     1081 gtaattaagt gggatgtaga ttctatagac tggaaggagc ttggaccaaa tcatgtaatt
     1141 gaaaaagtaa taaaagaatc tcaacctggt tcaatagttt tattccatgc taatataaat
     1201 gatgtagata actatttgga tactataata agtcgactaa aaaaagatgg atacagtcta
     1261 gtcaaaatat ctgatttgtt gtataaagat aattatgtag ttgattccaa tggagtgcaa
     1321 aaaactaaga attaataagt aataaatgta tattttaagt aaagttgtga taaattatat
     1381 agtatatgtt agaaaagtgt cgataaaaag acggtgtcta ttttttgaca ctttttatac
     1441 atgtatgcaa tatcaataat tatagattaa attgattaaa aaagtgtcta ataattgact
     1501 tttttttgat gggtttaaaa atttagaaga attaatgaat gctgtaagac agtatttcca
     1561 aagggtataa gtttgtttaa aagttggcat agaaattgct ttgttaataa gcgatatgtt
     1621 ctatagaaat aaacaatatg ttaagacctt ataatagaaa aatagcatta acattatttg
     1681 aacagcttat aaaaaaatta atatacttaa gggggcgtac tagttgaaca aactatattc
     1741 tattttatta aaacaacacg ttggtggtcc agataagcca gtagtaagtg ttggagatgt
     1801 agtaaaaaaa ggaactctaa tcgcagaacc aactggatta ggggcaaata tttatgccag
     1861 tgttagtgga aaaataagtg aaataaatga ccaagcgata gtaattgaag ctgatgaagt
     1921 acaagaagat acttttgaac cattaaaagg agaaggtata cttgacttaa ttaaagaagc
     1981 tggagtagta ggaatgggtg gagcaggatt ccctactcat gtaaaattaa acatagattt
     2041 aaatggtgga acaatattag caaatgcagc tgaatgtgaa cctttattag ctcataatat
     2101 aaaagaaata gaagaaagac cagaaatagt ttatcaaggt ataaaatatg ctatggaagt
     2161 tactaatgct ggaaaaggta tgctagcaat aaaaagtaag catccaaaag caatagaagc
     2221 tttcaaaaaa gtaataaagc caggagacaa catagaagtt gcagaactag ttgatatgta
     2281 cccaatggga gaagagagag caatagtaag agacgttcta ggaaaattac ttgagccaac
     2341 tcaattacct tcagaggcta atgcagtagt aataaacgta gaaacactaa ctagaatagt
     2401 agaggctgtt gagcaaaaaa gaccagttat atctaaaaat ataactgtag ttggtcaatt
     2461 aaacagtgga aaagaatcta tagtgtttga agatgtgcca attggaacaa ctgtcggaga
     2521 attaattgaa agagctggtg gaataaaagg tgaatatggt gaaataatcc taggaggacc
     2581 tttcactggt aaagctacta ctttagatgc tccaataact aaaactagtg gtggtataat
     2641 agttactatg ccatttgtta atgaaaagag aaaaatgggt cttttagttt gtgcatgtgg
     2701 acctaatgaa gaaagaatga gagatatagc aactaaaatg ggagttactg atatagtatc
     2761 agttcaaaaa tgtaaacaag ctcaagaaat taaaggtgca ttaaaatgtg aaaatccagg
     2821 acactgtcca ggacaagctc aaaaatgtat tgaatttaag aaagctggag cagaagttat
     2881 attaatagga aactgtactg actgtagtaa cacagtaatg ggttctgcac caaaattaaa
     2941 attaggtgta tatcatatta ctgaccatgt aatgagaaca gttaatcacc cattaataag
     3001 aagaataaaa ggtgacgtaa gattaaaaat aaaataatag ataaatagaa gttagacatc
     3061 tttagaagta aaacttaaaa gttacttcta aagatgtttt ttaatgtatt ttgagggcaa
     3121 tatatattgt aaaataaatg gaaaaaatag acaaaatatg ctagatattg tataatgata
     3181 acaatagcta ttacaattaa tattggacaa aaataatgat actcaagtat gtggtttgta
     3241 ttaatatgca tattatatta agtcaactat ccgatagagt aaaaggtttt tgtgatattt
     3301 gaagatactc tatagtatct tttttattat aaaaactaat ttttgtcttt tattagataa
     3361 gtatctagga tactaattta tattttattg agttgttaaa aaagtgcact taaaaagtta
     3421 aaaaagaaaa attaaaaaac ctatttatag aaactgtgaa gtatatctta aacctgggca
     3481 cttaaaagat atatggagtt agtagtgcaa cctgctataa atataaatag gagtaacttt
     3541 taattgtcaa aaatatataa aataaataca taaataagta attaatagaa gatagatata
     3601 aatatatttt tgaaatgctc cctgcattca taacgagtgt agagtggcag atatctgccc
     3661 aaaactcctt taattgctgg aagttcctaa agctaactaa accacaacat aatcatgaaa
     3721 taaggataag tgtgatagag tgtgcattta ataagcactt gaggttacga aagtagaaaa
     3781 aattagttag atagcataag gttaaatcct aagtgccttg aaaatggata atcagcaggt
     3841 aagcttcgaa aagaagaaac ttcaacgact agagagaaat ctcgtaccta caagtgtggg
     3901 gaaatgggga gtacctaatt tatggttagg taatgatata gtctgtgctt aatagaaata
     3961 ttaagaagtt cataggagaa ctggttagga gtaacgaccc taattgaaca cactaaagat
     4021 gtattattga ataagattca tataatattt aataatttta attatattaa gtgaatacaa
     4081 tatatgtttt tagtatcagg agaattttga aaacaaaaat taaaaaatca agtataatca
     4141 gtttagtaat tgcttttagt atggtattta ctgcatttac accaatagta tcttatgctg
     4201 acgaagtgac tagcaatgat acaatactta atggagaaga acaatcaaat ggtcagacac
     4261 cagatgtaga gaaaccatca gacggtcaga taccagatgg ggagaaacca tcagacagtc
     4321 aagtaccaga tggggagaaa ccatcagacg gtcaggtacc agatggggag aaaccatcag
     4381 atggtcagat accagatgga gagaaaccat cagatggtca gataccagat ggagagaaac
     4441 catcagatgg tcagatacca gatggagaga aaccatcaga tggtcagata ccagatgggg
     4501 agaaaccatc agatggtcag atgccagatg gagagaaacc atcagatagt cagacgccag
     4561 atgtagagaa accatcagat ggtcagatgc cagatgggga gaaaccatta gatgagcaaa
     4621 ctccagaaga agaaaaacca ttggaagaag aaatagttat agaggaaatg agtttaaaac
     4681 aagatattga taaaattctt gatatgactt tatctcaaat aaataaaata gtatataact
     4741 tttgggaaga tgaagaagat gtaaaggcag atgaacaatc agaaattaac caagttttta
     4801 cttcagaaga ctcatttatt agcttatggt atgacaaaaa agcaaaggtg aaaaattctt
     4861 gtttactaaa agaaatagat ggttcagata gaccttatca tgatttaaga tttgatgata
     4921 ttacaaagac tttaactttt gactatgtaa taaagggtct tgtaggagca agctcaaaag
     4981 acgacaaata tgtaatagaa ggtgaagatg gagagcaaac tgcattttgt tacaataatc
     5041 atttaagacc accaagtagt aatggaaaga gtccatattt accagcagaa gattttaatg
     5101 gtcaagaaaa ccaaaatgaa gaagcagtta aatctatact atatgcaggt tctgaatttg
     5161 atggatttgg atataagcaa caatttaatt taggcgaaga agaaaatgac ataatgactt
     5221 actcagctac tcaaagtgca atttggatta ttcttggaca aatagatgaa caagaaagac
     5281 ttaagcagta tcaaggctct atagaccttt gtgataaact tatagaaaga gcaaaaactg
     5341 aagaggaaag agctgagtat cagaagagga aagaagtagc gataaataca aaagcttatt
     5401 tagaagcatt gttaaaagca ggtcgtgaag agttaaagcc aaatgataca ggaaaaccta
     5461 gtttgagtaa tggtttaact aagattaact ttgaaaaaaa tgaagatggt acttatgaaa
     5521 cagaagctgt agccttagta ggttatagtg gtgtagtaaa gttaaaacta ccagatggag
     5581 taactgctta tgatgtagat ggtaatataa taggtacagg cgaagtagaa atttcaacgc
     5641 aacagaaatt taaacttaag agtgttggaa aaccagatgc caaagctaat atttctgctg
     5701 tatcttatga ttatattttt ccaaaagcta ttcaatatta taaggcagtg cttgatttag
     5761 gacaaaaaga ccattcatct tcattaccat ctagcaagca aaatttatta agttatacaa
     5821 tagagaaaaa gaatggtcaa gaagttaact ttaatatagg tttaccaaca gatgatgatg
     5881 atgttgtaaa tccaccagtg ccaccaatag atgatgatgt tgtaaatcca ccggtgccac
     5941 caacagatga tacaaatgga cataagccaa aaccatcacc accaatagat gatacagtaa
     6001 taaatccacc agtaccacca atggatgata caataataaa tccaccagta ccaccaacgg
     6061 aggatgcagt gttaaatcca ccagtgccac caatggagga tgcagtgcta aatccaccag
     6121 tgccaccaac agatgataca gtgttaaatc caccagtacc accagtaagt gatacagtgg
     6181 aaaaaactcc agaactatca agagatgata caatagtaaa atcaccaaaa acaggtgatg
     6241 aaactcagct aatgtcatat gtatttatta gtgtcattgc aatttgtgga ttagcgtatc
     6301 aatgtaaaat aaaaagaaat taatctagat tattattaag tttaaaataa aaagtctcaa
     6361 attgatttat aagtcatttt ggggcttttt tatgtaaata taatatgcaa atatagtttg
     6421 tttaaaaata tctattttaa ttatattctt aaatttatga ttggatacag gcattcatta
     6481 attgtttgat gaatagaaaa atgttttgaa ggtgaaaata gtattgaaaa aataatatac
     6541 taagggtata attcatttga tttcatttgt ataaatttaa ttataaattt taaagtgatt
     6601 atataaattt aaatgctaat tacttaataa attttaataa taattattta ataaataatt
     6661 attataatat gctagaataa atatatataa ataagttttg atgaggtact actataaagg
     6721 ggggacttta tgttttcgat accagaagta aaaaaagtag aagaggtaat ggatacaaaa
     6781 tttacaacta tagacgaaga cacaagaatt gaagatgcaa taaaagaaat gataaaaagc
     6841 aacacaaaaa ctctaatggt aatagattca tctgaccaat taaaaggcat tatatccatg
     6901 acggatatac ataatcttta tgagatgcat aaaaagtatg aaggtcaacc agtaaaactt
     6961 ataatgaaga aggatgtaat atatgtcaac gaaggtttaa ccttagatga atgtagagat
     7021 ataatgatat taaaaaatat agggattctt cctgttctta gagataataa aattattgga
     7081 gttctaaaac aagagcacat tagggactat ctatatatgc atttagaaga ttatggatta
     7141 actttgaagt atattatagg tcaaattaaa gaaggaattt gtgctataaa taacgaaggt
     7201 gttgttatct tgtggaataa gtttatggag gaaaggtatg atataaagtc tgaagatata
     7261 gttggtcgac cgatgaatga atttttggaa aatactattt cagaaaaagt actaaatagt
     7321 aaagttggaa tgagtgactt gtattttaca gataaaaaag aaaatatgta tgcattagtt
     7381 catgcaaatc ctatatttta taaagaagaa tttatcggtg ttgtatgtac agaagttgat
     7441 gttacagaag ctaagatatt agctcttgaa cttgaaaaag taaatgatac attaaaatat
     7501 ctaaagaacg aagtcaaaaa tttatctaaa ggtagttttg ataagatttt aggtaaaagt
     7561 tataaactgg aaaaatcgaa agcgattgca aaacaagtgg ctagaacaaa tagtagtata
     7621 tttatttggg gcgaaagtgg tacaggtaaa gaagtatttg caagagctat tcatgattat
     7681 agtgagagaa aagggcaatt tatacctgtt aactgtagtg ccataccaaa tgagttattt
     7741 gaaagtgagt tttttgggta tgaatcaggt gcatttacag gagcaagtaa aaagggtcga
     7801 ataggtatat ttgaacttgc aaaagatgga actgtatttt tagatgaaat tgccgattta
     7861 cctcttagta tgcaagcaaa gttgcttaga gtattacagg aaaaagaaat aagaagagtt
     7921 ggtggagata ccacaataaa aataaatcct agaataatat cagctaccaa taaagatttg
     7981 agtaaaatgg taaaggcaga aaaatttaga gaagatttat actatagatt aaatgttgtt
     8041 gaaataaaga ttcctccact tagagagaga aaagaagaca taggattatt ggttcatagc
     8101 tttttagagg agatatgcaa acaaaataat aagcctgttc ttacaattag caaagatgta
     8161 atagatatat ttcagaatta tagatggaaa ggtaatatta gagaattaaa aaatacgatt
     8221 gaaaatattg ttgtattgtc acaaaattct aaaatagatg tggatgatgt accaagttat
     8281 atgatggatt ctacaaataa tagtactgaa gaagaagagt atcctttaga tttaacgaag
     8341 gcaactcaaa aaattgaaat aaagaatata accaaggcac ttaaaatgtc taatggaaat
     8401 aaagctaaag cagctaagat attaaatata cccagaacaa ctttgtatta taagatagac
     8461 caatacaaaa tagatgtgtc taaaatatga cacatgacta aattttgaca aaatgataaa
     8521 ctttaattta aaacctagta aagccaacgc ttgtataagt gttgtgtata tagatgtgtt
     8581 aaaaaaaatg aaaaagtgtt gattaattga cacttttttt gtttgagaaa atgtttacaa
     8641 atacaaaaaa taacatattt taaataaata tttatataat atttggctaa aaatagcgat
     8701 agatgaagaa tctattatag aaaaaatctt tttgttaata agttggcata ggaattgctt
     8761 ataatataaa gtgactttaa gtgagaaaaa aataatatta atcataacaa attatgtagg
     8821 ttttaattta cttaatttct cagttaaggg gttacctgtt gaggaattga gatttaaaat
     8881 aataaataag aaaataatgt aaggaggtat tttatactat gtcaataact ttagaaacag
     8941 ctcaagccca tgcaaatgac ccagcagttt gttgttgtag atttgaagcg ggaacaatta
     9001 tagcgccaga aaacttagaa gatccagcaa tatttgcaga cttagaggat tctggattat
     9061 taacaatacc agaaaatgga ttaactatag gtcaagtact aggagctaag ttaaaagaaa
     9121 ctttagatgc actttctcca atgactacag ataacgtaga aggatacaaa gcaggagagg
     9181 ctaaagaaga agtagtagaa gaaacagtag aagaagcagc tccagtatca gaagcagcag
     9241 tagttccagt aagcacagga gttgcaggtg aaacagttaa aatacacata ggtgaaggta
     9301 agaacataag cttagagata cctttatcag tagctggtca agcaggagtt gctgctccag
     9361 tagcaaacgt tgctgctcca gtggcaagtg cagcagcaga agtagctcca aaagttgaag
     9421 aaaagaaact tttaagaagc ttaactaaaa aacactttaa aatagataaa gttgaatttg
     9481 ctgatgaaac taaaatagaa ggaactactt tatacatcag aaacgcagaa gaaatatgta
     9541 aagaagctaa tgaaactcaa gagttagttg tagatatgaa gttagaaata ataactcctg
     9601 ataaatatga aacttacagt gaagctgtat tagatataca accaatcgct actaaagaag
     9661 aaggcgaatt aggttcaggt ataactagag ttatagatgg agctgtaatg gtattaactg
     9721 gtacagatga agatggagtt caaataggtg aatttggttc ttcagaaggt gagttaaata
     9781 ctactataat gtggggtaga ccaggtgctg ctgacaaagg tgaaatattc atcaaaggtc
     9841 aagtaacaat aaaagcagga actaacatgg aaagaccagg acctttagct gctcaccgtg
     9901 catttgacta tgtaactcaa gaaataagag aagcattaaa gaaagttgac aactctttag
     9961 tagttgatga agaagtaata gagcaataca gaagagaagg taaaaagaaa gttgttgtta
    10021 taaaagaaat aatgggacaa ggtgcaatgc atgataacct aatattacca gttgagccag
    10081 ttggtacatt aggagctcaa ccaaacgttg acttaggaaa catgccagtt gtattatctc
    10141 cacttgaagt attagatggt ggtatccatg cattaacttg tataggacct gcatcaaaag
    10201 aaatgtcaag acattactgg agagagccat tagtaataag agctatggaa gacgaagaaa
    10261 tagatttagt aggtgttgta tttgttggtt ctccacaagt aaatgctgag aaattctatg
    10321 tatctaagag attaggtatg ttagttgaag ctatggaagt tgatggagct gtagtaacta
    10381 ctgaaggttt cggaaacaac catatagatt tcgcatctca catagagcaa ataggtatga
    10441 gaggtatacc agtagttggt gtaagtttct cagctgttca aggtgctcta gttgttggta
    10501 ataaatacat gactcacatg gtagacaaca ataagtctaa gcaaggtata gagaatgaaa
    10561 tattatctaa caacacttta gctccagagg atgctgttag aataatggct atgcttaaaa
    10621 atgctataga aggtgtagaa gttaaagctc ctgaaagaaa atggaatcca aatgttaaat
    10681 taaataacat agaagctata gaaaaagtta caggagaaaa aatagtatta gaagagaatg
    10741 agcaatctct accaatgagt aagaagagaa gagaaatata cgaaaaagac gaaaactaaa
    10801 atttagatac taactaagat ttagacaata acataaattt agatccaagt ttaaaacagt
    10861 atagagaggt agaattataa tggataggtt aaaatatatc tcatcagaga ctttttatga
    10921 aggtattata gttgacatca aaggtggtgg agtgactata gacctaaaag gtagacttgg
    10981 acaatttaaa atacctaata gaatgcttat aactgactac gaacttaaga taggtcaaga
    11041 agttggattc atgctaagct atcctgaggt attaagtcca gagcctaatc aagagtatgt
    11101 agaaaatatt aggagagaaa aagaaaaaca agcagaaatg caatcaaaac aagaagaaaa
    11161 ataataaata atcgtttgaa aatagaaggg agaggaaata tatgagcctt acaacagtac
    11221 aaggacttca atctgaaata ttcgttccaa taacacctcc tccagtttgg actcctgtaa
    11281 ctaaagaatt aaaagatatg actatagctt tagctacagc agctggtgtt catttaaaat
    11341 ctgacaagag atttaatcta gcaggagata ctacatttag agctatacct aatacagcta
    11401 cagttgatga aatgatggta tcacatggtg gatatgacaa tggagatgta aacaaagata
    11461 taaactgtat gttccctata gatagattac atgaattagc tgcagaagga tttatcaaag
    11521 gagtagctcc tatgcactat gcattcatgg gtggtggagg aaaccaacat gtcttcactg
    11581 aagaaactgg tcctgctatc gctgctaaac ttaaggaaga gggagtagac ggtgtagtta
    11641 tgacagctgg ctgaggtact tgccatagaa ctgccgtgat cgtgcagaga gcaatagagg
    11701 aagctggaat acctacaata ataatagcag ctcttcctcc agtagttaga caaaacggaa
    11761 ctcctagagc agttgctcca ttagttccaa tgggtgctaa tgctggtgaa ccacataata
    11821 tagaaatgca aacacatata ttaagagata cattagagca attagttgca ataccatctg
    11881 ctggtaagat agttccatta ccatacgaat ataaagctca cgtttaaaat gtaatttata
    11941 tttgaaaata tagaaccctt ctcctacacg gttaggagag gggttttaag aacaaaggtg
    12001 gatattatgg aagaaaaaat aatgagaaga cttgttataa aaccgtttca tatgaatgaa
    12061 gttaatttcg gttcaaaaac ttctataaag aaagatgtac ttacaataga tttatcaagt
    12121 atagatgaaa taaaagaaag agaagagtta ataacagaca taaaagtaga cattataaaa
    12181 cctggagatt atgacagaga aattaatact attatggaca ttataccaat atcaacaaaa
    12241 gttttaggca gactgggaga gggtattaca catactttaa cgggtgtata tgtaatgtta
    12301 actggtgcag atgaagatgg aaatcaaatg catgaatttg gttcttcaga aggaattttg
    12361 aaagaccaaa tggtatttgg gagatatgga actccatcag ttgaagatta tataatccat
    12421 gtagatgtaa ctttaaaggg aggtttacct tttgaaagaa cacttccact tgcagcattc
    12481 agagcatgtg atgattttat acaagaaata agagcgtctt tgaaaatgga agacggaaga
    12541 aatgctactc aagttcgtga atattttgac aaaattcgac caaatgcaaa aaaggttgtc
    12601 atagtaaaac aaatagcagg tcaaggtgct atgtatgaca atcagttatt ctcaaaggaa
    12661 cctagtggat ttgaaggagg aacatcaatt atagatatgg gaaatgtacc tatgattata
    12721 tctcctaatg aatacagaga tggtgccctt agagctatga cttaagagag gtggatatta
    12781 tgggaatagg accttcaaca aaagaaacat ctcttcatca ctttagagac ccacttttag
    12841 acatagttga aagtgataag gatgtagatt tacttggagt aatagtagta ggaactccag
    12901 atggaaatga aaataaaacc tttgtgggac aaagaacggc cgcttggctg gaagccatga
    12961 gagtagatgg agctatagtt tcatcagatg gttggggaaa ctcacatgtt gactacgcta
    13021 acacatttga ggaaatagga aaaagagata tacctgtagt tggagttaca tttaatggga
    13081 ctcaagctaa gtttgttgta agcaatcaat atatggatac catagttgat atgaacaaat
    13141 caaaagaagg tattgagaca gaagtagttg gagaaaacaa cacaaatgaa atagatgcta
    13201 aaaaagcttt ggcattttta aaacttaaaa tgagaaaaca tggataaaaa atttaagtaa
    13261 gggaatgagt tttgtgggaa tttctacatc aatgaaaaac acatcacttc atatatttag
    13321 agacccgtta ctagatttga tagataatga ttctgacata gacttgttag gagtggttgt
    13381 agttgggaca tcagagcaca atgaatggaa aacattttta gcaactagac ttggaagatg
    13441 gattgaatca ttgcgacctg atggagtgat tataacttta gatatggcag gaaaccagca
    13501 tattgatttt acaaatgcta tagcagagtt tgtaaaatca gatataccta cagtaggtct
    13561 tactattatg ggagcagatg gattagtaat taccaatcca tatttggata aagctaccat
    13621 aattgattat aaaaagacaa caggctacat agaaacagaa gtagttggag acaatcacat
    13681 ggatgaagtt gatgttaaaa aagcattggc tttcctaaaa ttaaaaatgc gtaaagacgc
    13741 aaaataaacc taagggggaa tttgatatga aatttagcag aagtatacaa gctatagact
    13801 ctcatacagc aggtgaagca actagaatag ttgtaggtgg aatacctaac ataaaaggaa
    13861 attctatgcc tgagaaaaaa gaatatttag aggaaaatct agattattta agaactgcta
    13921 taatgttaga gccaagagga cataatgata tgtttggttc tgtaatgact caaccttgtt
    13981 gcccagatgc tgactttgga ataatcttca tggatggtgg cggatacctt aacatgtgtg
    14041 gccatggtac aataggagct atgacagcag ctatagaaac aggtgtagtt ccagcagtag
    14101 agcctgtaac tcatgtagtt atggaagctc cagcaggaat aataagagga gacgttacag
    14161 ttgtagatgg aaaagctaaa gaagtatcat tcttaaacgt accagctttc ttatataaag
    14221 aaggtgttga agttgactta ccaggtgttg gaactgttaa atttgacata tcatttggtg
    14281 gaagcttctt tgctataata catgcaagtc aattaggttt aaaaatcgaa cctcaaaatg
    14341 ctggtaaatt aactgaacta gctatgaaac ttagagatat aataaatgag aaaatagaaa
    14401 tacaacatcc aactttagct catataaaaa ctgtagactt agttgaaata tatgatgagc
    14461 caactcatcc agaagctact tacaaaaatg tagttatatt tggtcaaggt caagttgaca
    14521 gatctccatg tggaactgga acaagtgcta aattagctac tttacatgct aaaggtgaat
    14581 taaaagtagg agaaaaattc gtatatgaaa gtatattagg aactttattc aaaggtgaaa
    14641 tagttgaaga aactaaagtt gcagatttca atgctgtagt acctaaaata actggttctg
    14701 cttatataac tggatttaat cattttgtaa tagatgaaga agacccactt aaacatggat
    14761 ttattcttaa ataagtaata aataaattag gaaattaaaa tagaatagta gccacttgtc
    14821 ggttgctatt ctatagcttt taggctataa gattcagaat atatgaaaaa ataaaaaaat
    14881 aggaggatgg attatggtag caatcttaaa ggcattatat gtagtattcg cagcaatctt
    14941 tgcagttgtg tttggtaaag acgtagctaa agcaaacaaa gaggggaaac tagaagaaca
    15001 aagtactcca aaagttgctg taacaggatt tataacagac ttctttgata cattaggaat
    15061 agggtcattt gctccaacaa tagcaatgtc aaaggcttta aagttaaata taccagataa
    15121 aaaaatgcct ggtacattaa acgtagcaca tactattcca gttgttacag aagcattcat
    15181 atttacatca atcataccag ttgatggtgt tactttagta tctatggttg tggcagcagc
    15241 agttggttca tatataggtg caggaataat agctaagatg gacgagagaa aaatacaact
    15301 tgttatgggt gtaactttag cattaacagc aatattaatg ttattaggtc atccttggat
    15361 taacgtatta ccaggtggag gaaatgaatt aggtcttact ggtgctaaat tagtaatagg
    15421 tgtaataggt aactttatac taggagcttt aatgacagct ggtataggtc tatatgctcc
    15481 aggtatggca atggtttatt tcttaggaat gtctgcaaaa gttgcattcc caatagttat
    15541 gggttcttgt gcattattaa tgccagttgc aagtatgaaa ttcataaaag aagatgcata
    15601 tactaaaaaa gcatcagtta taatagctat atgtggttta ataggagttt tcgtagctgc
    15661 atatatagta aaaactctac caatggatat attaaaagca ttagtaatag tagttatagc
    15721 ttatacttct gctacaatga taatggctgc aaataaaaac aaaaagactg cttagttaaa
    15781 gactacttag tgtttattaa atattacagc taataataaa gattataaac tgctttaaaa
    15841 tattgaatta atattttagg gcagtttttt atttactaaa ttaatataaa gcaattaaca
    15901 tgccaattat gtaatatata tagtgattta gtagatttaa tgtaaaaaac gttgtatttt
    15961 taaagaataa agcataattt aaagctaaat atttaaattt tataatcaat aagttcaata
    16021 aatgttcata tattaataca gattaataaa aatataacct ctaataagtc taatat
//
LOCUS       DAEMHZ010000095        15979 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000095 DAEMHZ010000000
VERSION     DAEMHZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.095"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="KPK72_002668"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="KPK72_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG2489869.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="KPK72_002669"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="KPK72_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG2489870.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="KPK72_002670"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="KPK72_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG2489871.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="KPK72_002671"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="KPK72_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG2489872.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1740..2438
                     /gene="rplA"
                     /locus_tag="KPK72_002672"
     CDS             1740..2438
                     /gene="rplA"
                     /locus_tag="KPK72_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG2489873.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2490..2618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2661..3167
                     /locus_tag="KPK72_002673"
     CDS             2661..3167
                     /locus_tag="KPK72_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG2489874.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPVRILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3225..3590
                     /gene="rplL"
                     /locus_tag="KPK72_002674"
     CDS             3225..3590
                     /gene="rplL"
                     /locus_tag="KPK72_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG2489875.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3983..4771
                     /locus_tag="KPK72_002675"
     CDS             3983..4771
                     /locus_tag="KPK72_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2489876.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAH
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5242..8958
                     /gene="rpoB"
                     /locus_tag="KPK72_002676"
     CDS             5242..8958
                     /gene="rpoB"
                     /locus_tag="KPK72_002676"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG2489877.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9000..12485
                     /gene="rpoC"
                     /locus_tag="KPK72_002677"
     CDS             9000..12485
                     /gene="rpoC"
                     /locus_tag="KPK72_002677"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG2489878.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12774..13196
                     /gene="rpsL"
                     /locus_tag="KPK72_002678"
     CDS             12774..13196
                     /gene="rpsL"
                     /locus_tag="KPK72_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG2489879.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13320..13790
                     /gene="rpsG"
                     /locus_tag="KPK72_002679"
     CDS             13320..13790
                     /gene="rpsG"
                     /locus_tag="KPK72_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG2489880.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13839..15905
                     /gene="fusA"
                     /locus_tag="KPK72_002680"
     CDS             13839..15905
                     /gene="fusA"
                     /locus_tag="KPK72_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2489881.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6254 a         1988 c         3250 g         4487 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa acaatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt ataaagtggg aggctagaag ccgaaattaa ccacgaggag gaaaataaaa
     1741 tggctaaaaa aggtaaaaga tatgcaggtg cattacaaaa agtagataga actaaatttt
     1801 atgatgcatc agaagcatta acattagttt cagatatagc tggagctaaa tttgatgaga
     1861 cagtagaagc acacattaaa ttaggtgttg actcaagaca tgctgaccaa caagtaagag
     1921 gtgcagttgt attacctcat ggaactggta aaacaaaaag agttttagtt tttgctaaag
     1981 gtgaaaaagc taaagaagct gaacaagctg gtgctgactt tgtaggagct gaagaattag
     2041 ttcaaaaaat acaaggtgaa aactggtttg actttgatat agtagttgct actccagata
     2101 tgatgggtgt agtaggtaga ttaggtagag tattaggacc taaaggttta atgccaaacc
     2161 ctaaatcagg aacagttaca tttgatgtag ctaaagctat agatgaaata aaagctggta
     2221 aagttgaata cagattagac aaaactaaca taatacacgt tccagtagga aaagtatcat
     2281 ttggtggaga aaaattaact gaaaacttta ctgcattaat ggatgctata ataaaagcta
     2341 aaccagctgc tgctaaagga caatatttaa gaagtataac agtagcttct tcaatgggac
     2401 ctggagttaa aataaaccca gcaagaacag ctgaataata aaactgttga cacaggttga
     2461 caaaaatgtt attatatcac ttgtcaatgt aaaaaagaat agatttgccg tagatagtgg
     2521 gtgcgagagc ttaatatccc gccgaggttt tagataaatt gagattacta aagcttttga
     2581 tgtctacttt tggcaacaaa agctttttat aatgttgcgg taaatactaa gcctatttgg
     2641 tgaaggaggt gcacatctat atgagaaaag ctatagaaat taaatcagaa gttgtttctg
     2701 agatagttga aaagttacaa aaatcttctg ctgctgttgt tgtagattac aaaggattaa
     2761 cagttgaaga agtaactgag ttaagaaaac aaatgagaga agctggtgta gattacaaag
     2821 tatacaaaaa tactttagtt agaagagcag ctaaagaagt tggtatagag caatttaatg
     2881 atgaattatt agttggaact aatgccatag catttggata tgatgatcca gttgctccag
     2941 ttagaatatt aaaaggattc atggattctc atccaaaaat gaaattaaag atgggtattg
     3001 tggaaggtgc attctatgat gaatctaaga ttgttgaaat ggctaacata ccatcaagag
     3061 aagttcttat cgctaaatta cttggtagct taaaagctcc agtatcaaac ttcgcatact
     3121 taatagatgc aatagcgaaa aaggcagaag gtcaagaaga agcttaattg cttaactaaa
     3181 aaattataaa tattaatttg aaataaaatt cggaggtgct aaaaatgaca atagaacaaa
     3241 tattagaagc tatagaaaat atgaaagttt tagaattaaa tgaattagtt aaagctgctg
     3301 aagaaaaatt cggtgtatca gcttcagctc cagtaatggt agctggtgca gctgctggtg
     3361 gaccagctgc tgaagaaaaa actgagtttg atgtagtatt aactgacgtt ggttcttcaa
     3421 aagttggagt tataaaagca gttagagaaa taacaggatt aggattaaaa gaagctaaag
     3481 aagtagttga caatgctcct aagacagtta aagaaggagc ttctaaagaa gaagctgatc
     3541 aaataaaaga gaaattagaa gctgctggag ctaaagtaga agttaagtag tttctttaaa
     3601 aagataccca ttgggtatct ttttttattt ataaaaaact ataaagaaag tatttggaat
     3661 tataaagaaa gtatttttat aaaataaaaa aaattttata taatagtact aaattaaggg
     3721 tatcatatat gatgtaatgg tattttaatt taatattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctatata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgttttgta aataaaatgt aataaataat taattaacaa
     3901 atataaattt taaaataagt tagatattta taaaaatcga aatttgtagg atttaaagga
     3961 catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag tagttactgc
     4021 agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg gagcaactgt
     4081 gtacttagca gctcgttcag aagaattagc tcatgaagtt ataaataaaa taagtgcaga
     4141 aggtggttgt gctaagtttg tttactttaa tgctcgtgaa gaagagacct ttacctcaat
     4201 gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca attttggttc
     4261 aacaaaccct tctcttgaca aagaccttgt aactggggat acagataatt tctttgacac
     4321 agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc ctcatatgat
     4381 aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat tacctgattt
     4441 atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc aaaacatagc
     4501 tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc ttattgcaac
     4561 taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa agcatgttcc
     4621 tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct atgctagtga
     4681 tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg gtttacctac
     4741 tccacaattt gcagataata tattaggata attaaattac taatatgcaa tgaaagctta
     4801 tgcattagtt gtagtagaaa tgatattttt ataaaggcca tagatataat ttatattgga
     4861 aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtctttattt tgtatatttt
     4921 aataaattgt atatatttgt ataaatgtaa ttatttacaa tcattttaat atggaattat
     4981 aattaaatag gggggatggt acttgtataa aattttttat agaaaattag acctataaat
     5041 gaaaaatatg gattttatat tggaaaattg tacataatac ataatatatg cttaaaataa
     5101 tttacaaaaa atcaaaaata atgcatagtt tacatataag ataaaaaata tattgaaaaa
     5161 tacttgacat tatgctaaaa ttattagatg cgttagaatg aactattgtt ttatttttaa
     5221 ttattgagag gtgaaaagtg aatgccacat cctgtcacga taggtaaaag aactagaatg
     5281 agcttttcta aaattaagga aatagctgat gtgccaaatc ttatagaaat tcaagtagat
     5341 tcctatgagt ggtttttaaa agaaggttta aaagaagtgt ttgatgatat ttcaccaata
     5401 gaagattata ctggtaatct tatattggaa tttgtcgact attctctaga tgataaacca
     5461 aagtacgata tagaagagtg taaagagaga gatgctacat attgtgcacc tctaaaggtt
     5521 aaggtaagac ttataaataa agaaacaggt gagataaaag aacaagaagt gtttatgggt
     5581 gacttccctt taatgacaga aagaggaact ttcgttataa atggagcaga aagagttata
     5641 gtaagtcaat tagttagatc tcctggtgtt tactatgcgg aagaaagaga taaaacaggt
     5701 aagagattga tttcatctac tgtaattcct aatagaggag catggttaga atatgaaaca
     5761 gattctaatg atgtaatatc tgtaagagtt gatagaacaa gaaaacaacc agttacagtt
     5821 ttacttagag ctttaggaat aggaacagat gcagagataa ttgacctttt aggagaagat
     5881 gagagattat ctgctacatt agaaaaagat aatactaaga cagtagaaga aggtcttgta
     5941 gagatataca aaaaattaag accaggtgaa cctcctactg tagaaagtgc atcatcttta
     6001 ttaaatgctt tattctttga cccaaaaaga tatgacctag caaaagttgg tagatataaa
     6061 ttcaataaga aacttgcttt atgttataga ataatgaata aaatttcagc agaagacata
     6121 ataaatccag aaactggaga agtatttgta aaagctggag aaaaaataag ctatgatttg
     6181 gctaaagcca tacaaaatgc aggtataaat gtagtaaatc tactaatgga tgatgataaa
     6241 aaagttagag ttataggaaa taattttgtg gatattaagt ctcacattga ttttgacata
     6301 gatgatttaa acataaaaga aaaagtacat tatccaactt taaaagaaat tttagatgga
     6361 tatagtgatg aagaagaaat aaaagaggct ataaaatcaa gaattaaaga acttatacca
     6421 aaacatatat tattagatga tataatagct tcaataagtt atgagttcaa tatattctat
     6481 aatataggaa atattgatga tatagatcac ctaggaaaca gaagaataag atctgtaggt
     6541 gaattgttac aaaatcaagt tagaattggt ctttcaagaa tggaaagagt tataaaagaa
     6601 agaatgacgg ttcaagatat ggaagctata acgcctcaag cgttagttaa cataagacca
     6661 gtttcagctg caataaaaga attttttggt agttctcagt tatctcagtt catggatcaa
     6721 acaaatcctc tatctgagtt aacgcataag agaagattat cagcccttgg gcctggaggt
     6781 ctttcaagag aaagagctgg attcgaagtg cgtgacgttc accattcaca ctatggtaga
     6841 atgtgtccaa tagagactcc agagggacca aatatcggtc ttataaactc tctaggaact
     6901 tatgcaaaaa taaatgaatt tggatttata gaatcaccat atagaaaatt tgataaagaa
     6961 acatcaacag ttactgatga aatacattat ttaactgctg acgaagaaga tttattcgta
     7021 agagctcagg caaatgagcc attaacagaa gatggtaaat tcgtaaacca tagagttgtt
     7081 tgtagaactg taaatggtgc tgttgaaatg gttcctgaaa gtagagtaga ttacatggat
     7141 atatctccta agcaggttgt atctgttgcc actgctatga tacctttctt agaaaatgat
     7201 gatgccaacc gtgcccttat gggagcgaac atgcagcgtc aggcagtgcc tctagttaga
     7261 agagaagcac caattatagg aactggtata gaatacagag cagcaaaaga ctctggagca
     7321 gttgttgttg ctagaaattc tggtatagca gaaagagtaa cagcagatga aataattata
     7381 aaaagagaag atggaaacag agatagatat aatctactta agtttaaacg ttcaaactca
     7441 ggtacttgta taaatcaaac acctataata aataagggtg accagataat aaaaggtgat
     7501 gttatagcag atggtccagc aactgattta ggagaagttg cacttggaag aaactgtctt
     7561 atagcattca tgacttggga aggttataac tacgaggatg ctatattaat aaatgaaaga
     7621 ttagttaaag aagatagatt atcaacaatt catatagaag aatatgaatg tgaagctaga
     7681 gatacaaaac tagggccaga ggaaataact agagatatac ctaatgttgg agatagtgca
     7741 attaagaact tagatgacag aggtataata agaataggtg cagaagtaga ctcaggtgat
     7801 atattagttg gtaaagtaac tccaaaagga gaaactgaac ttactgctga ggaaagatta
     7861 cttcgtgcaa tatttggaga aaaggctaga gaagttagag atacttcact taaagtgcct
     7921 catggtgaat ctggtataat agttgatgta aaagtattta ctagagaaaa tggagatgat
     7981 ttatctccag gagtaaatga attagtaaga tgctatatag ctaaaaagag aaaaataaaa
     8041 gttggagata aaatggctgg tcgtcatggt aataaagggg ttatctcaag agtattacct
     8101 gaagaagata tgccattcat ggaaaatgga acaccactag acataatact taacccacaa
     8161 ggtataccat cacgtatgaa cattggtcag gttttagaag ttcatttagg acttgctgct
     8221 aagacattag gatggtatgt agcaacatca gtattcgatg gtgctaatga gtatgacata
     8281 atggatgctc ttgaagaagc tgggtatcct agagatggta aattaacttt atatgatggt
     8341 agaacaggtg agtcttttga caacagaata actgttggat atatgtacta cttgaaacta
     8401 catcacttag tagatgaaaa actacatgca agaagtactg ggccatactc attagttact
     8461 caacagccac taggtggtaa agcacaattt ggtggacaaa gatttggaga gatggaggtt
     8521 tgggctctgg aagcatatgg agctgctcac atacttcaag aaatacttac tgttaagtct
     8581 gatgacgttg taggtcgtgt tagaacatat gaagctatag ttaaaggtga aaatatacct
     8641 gaaccaggaa tcccagaatc atttaaggtt cttataaaag aacttcaaag tttatgctta
     8701 gatgtaaaag tattgacaga tgaagaccaa gaaatagaag taagagaatc tgtggatgaa
     8761 gatgatacaa taggtgaatt tgagctagat gttgtaaatc atatgggaga agttgaagaa
     8821 agtaatataa tagaagaaat tgaagatgat tttgcagaaa atgcagaaga tgaggatata
     8881 gagaatttag aagaattcac tgaagatgat ttatttgaag aagaaataga ttttgatagt
     8941 gatgactttg atatgtaaat ttaattaatg taaatctcaa atagaaggga gagaactcct
     9001 tgtttgaatt aaacaatttc gagtcgataa aaatagcatt ggcttctcca gaaaaaataa
     9061 gacaatggtc taggggagaa gttaaaaagc cagaaactat aaattaccgt actttaaaac
     9121 cagaaaaaga tggtcttttc tgtgaaagaa tatttggacc acaaaaagac tgggagtgcc
     9181 actgtggtaa atatagaaga gttagatata aaggtgtagt ttgtgataga tgtggagtag
     9241 aagtaactaa atcaaaagta agaagagaga gaatgggaca tatagagcta gctgctccta
     9301 tgtctcacat ctggtacttc aaaggtatac caagtagaat gggactttta cttgatatgt
     9361 caccaagatc gttagaaaaa atattatact ttgcctcata tgtggtagtt gatccaggag
     9421 agactggatt aaacgaaaaa caattgctta cagaaaaaga atacagaact gctcttgaaa
     9481 agtatggata tacttttact gtaggaatgg gtgctgaagc tgtaaagaca ttactacaaa
     9541 atatagattt agagcaacaa agtaaagacc taagagcgga gttaaaagat agtacagggc
     9601 aaaagaaagt tagaacaata agaagattag aagttgtaga ggcatttaaa aagtctggaa
     9661 ataaaccaga atggatgatt ttagatgcaa taccagtaat accaccagat ttaagaccaa
     9721 tggtacaact tgatggtgga agatttgcga cttcagacct aaatgattta tatagaagag
     9781 ttataaatag aaacaataga cttaaaagat tattagagct tggagctcca gatataattg
     9841 taagaaatga aaaaagaatg cttcaagaag ctgttgatgc attgatagat aatggtagaa
     9901 gaggtagacc tgtaacagga cctggaaata gaccacttaa atctttatca gatatgttaa
     9961 aaggtaagca aggtcgtttc cgtcaaaact tacttggtaa gcgtgttgac tactcaggac
    10021 gttctgttat agttgttgga ccagaactta aattttatca atgtggactt ccaaagaaga
    10081 tggcattaga attattcaag ccatttgtta tggataaatt agttaaagaa ggttatgcac
    10141 ataatataaa aagtgcgaaa tctatagtag aaaaagttaa gccagaagtt tgggatgttt
    10201 tagaagatgt tataaaaagt catccagttc ttcttaaccg tgcaccgact ctacatagat
    10261 taggtataca agcatttgaa ccaatcttag ttgaaggtaa ggctataaaa ctacatcctc
    10321 ttgtatgtac agcttataat gcagactttg atggtgacca aatggcagtt catgtacctt
    10381 tatcagtaga agcacaagca gaagcaagat tcttaatgct ttctgtaaat aatatacttg
    10441 ctcctaaaga tggttcacct ataactactc catctcagga tatggtttta ggttgctatt
    10501 atctaacaat agaagcccaa gatggagcta aaggaacagg tatggtattt aaagacttta
    10561 atgaactatt acttgcttat tacaataaat cagttcatct acatgcatta gtgaaactta
    10621 aggtaacact tgaagatgga agaagttcat tggttgaaag tactgttggt agatttatat
    10681 ttaatgaaaa tatacctcaa gacttaggtt ttgtagatag aaaagaaaat ccatttgcac
    10741 ttgaagttga tttcttagca gataaaaaat ctcttggtaa aataatagat aaatgcttta
    10801 gaaaacacgg aaatacagaa acagctgaat tactagatta tataaaagct ctaggattta
    10861 aatattctac attaggtggc ataacagttg ctgttgatga tatgagtgtc ccagaagaaa
    10921 agaaagtatt tatagctgag gcagaagcta aagttgataa gtatgaaaaa gcatacagaa
    10981 gaggtctaat ctctgatgaa gaaagatatg aaaaagttat agagacatgg acagaaacaa
    11041 ctgataaagt tactgatgct cttatgggtg gactagatag attaaataat atatatataa
    11101 tggcacattc aggagccaga ggttctaaaa accaaattag acagctggca ggtatgcgtg
    11161 gtcttatggc aaatgcatct ggtaaaacag ttgagatacc agttaaatct aatttccgtg
    11221 aaggtttatc agtactagaa tactttacat cttcacatgg tgccagaaaa ggtcttgccg
    11281 atacagctat acgtacagct gaatctggat atttaacaag aagacttgtt gatgtaagtc
    11341 aagatgttat tgtaagggaa atagattgtg gaacagaaga tactacagag atttatgcta
    11401 taaaagaagg aaatgaagtt atagaagaga tatatgatag aattgtagga agatatacta
    11461 tagaccctat attaaatcct gaaactggag aagttatagt tgaggctgat tcaatgatac
    11521 aagaagatga agcagaaact atagtagctt taggaattga aaaaattagg ataagaacag
    11581 ttcttaactg taaaactaat catggagttt gttctaagtg ttatggtaga aacttggcaa
    11641 caggaaaaga agttaatata ggtgaagcag ttggtataat agcagctcaa tctatcggtg
    11701 agccgggtac tcagcttaca atgcgtacat tccatactgg aggagttgca ggagctgata
    11761 taactcaagg tctaccaagg gttgaagaat tatttgaagc tagaaaacca aaaggattag
    11821 ctgtaataac tgaagtatct ggtagagttg aaattgatga aacaggaaag agaaaagaag
    11881 taaatgtaat accagaagaa ggcgagactc aaacatatgt aattccatat ggttctagat
    11941 taaaagttaa gcaaggtcaa atgctagagg ctggagaccc tctaacacaa ggatttataa
    12001 atcctcatga tatagtaaga gtaaatggtg ttaagggagt tcaagaatat atagttaaag
    12061 aagttcaaag agtgtataga cttcaagggg ttgatgttaa tgataagcat atagaagtta
    12121 tagtaagaca aatgctatct aaagtaaaag ttgaagaccc aggagataca gatttattgc
    12181 caggtggata tgaagatgta ttaacattca atgaatgtaa taaagatgct atagataaag
    12241 gtctaagacc agcagttgct aaaagagttt tacttggtat aactaaagca tctcttgcaa
    12301 ctgattcatt cttatcagca gcttctttcc aagaaacaac aagagtatta acagaggcag
    12361 caataaaagg taaagaagac cacttaatag gacttaaaga aaatgttata ttaggtaagt
    12421 taataccagc aggaacagga atgaagaaat atagaaatat agctgttgaa aaaattgaag
    12481 attaatagca tatagagtaa agaaatcttg acaaccatta tttgtggtgt taaaatagta
    12541 aagtgtgttg tttaaaagta cactaaggac ttgaacaagt tcttagtgac ttttagatgg
    12601 caataaatga gaactaaaaa gcatcaagct agaaaagtgc taatatcaca tatttatgtg
    12661 ttatagacat taaattgtaa gtaacgaatg ttacaatcag tcagtatgaa tattcatatt
    12721 gatgtagttg gcggtcactg ataagatcga aaatttggaa ggaggtgcag atgatgccaa
    12781 caattaacca attagttcgt aaaagtagaa aagcgctaga gaagaaatct actgctccag
    12841 cattacaaaa aggttacaac tctttaaata agaaagtaac tgacgctagt gccccacaaa
    12901 aaagaggggt ttgtacttca gtaaaaacag ttactcctaa gaagcctaac tcagctttaa
    12961 gaaaagttgc cagagttaga ttaactaatg gtatagaagt ttctgcttat ataccaggag
    13021 aaggacacaa cttacaagag catagtgttg ttcttataag aggaggaaga gtaaaagacc
    13081 ttccaggggt tagataccat atactaagag gtactctaga tacagcaggt gttgataaga
    13141 gaagacaatc aagatctaaa tacggtgcga agagacctaa agaagctaaa aagtaaaaac
    13201 aattaatgat tgattatagt tcggtgcttt aaatatatat atatttacgg catcacggca
    13261 ttaaatgatt tatgtcgagt acctatgatt taagttaaat aattaaggag ggaagcgaaa
    13321 tgccaagaaa aggtaatatt ccaaaaagag aagttttacc agatcctatg tatgggagta
    13381 aggtagttac taaattaata aataatctaa tggtagacgg aaaaaaagga aagtctcaaa
    13441 gaatagttta tgatgctttc gcaattgttg ctgaaaagac aggagaagaa gctttagaag
    13501 tatttaataa agcaatggat aatataatgc ctgttttaga agtaaaagca agaagagttg
    13561 gtggggcaaa ctaccaagtg ccaatcgaag ttagacctga aagaagacaa acattaggtt
    13621 taagatggct agtaaaatat actagagctc gtggagaaaa aggaatggtt gaaaagctag
    13681 ctaaagagat aatggacgca gctaacaaca ctggagcttc agttaagaaa aaagaagata
    13741 ctcataaaat ggcagaagct aataaagcat ttgctcatta cagatggtag gatatataga
    13801 tatcttataa aattgaggat atcataagga ggataaccat ggctagaaag tttcctttgg
    13861 aaagaactag gaatatagga atcatggctc atatagatgc aggaaaaact actactacag
    13921 aaagaatcct gttttatact gggcaaactc ataaaatagg agaaactcat gaaggagctt
    13981 ctcaaatgga ctggatggag caagaaaagg aaagaggtat aacaataact tctgccgcta
    14041 ctactgcttc atggaaagac catagaataa acataataga tactcctgga cacgtggact
    14101 tcacagttga ggttgaaaga tctctaagag ttcttgacgg ttcagttgct gtattctgtg
    14161 caaaaggtgg ggttgagcct caatcagaaa atgtatggag acaagctgaa acttatggag
    14221 tacctagaat agcttttgta aataaaatgg atatattagg tgcagatttc tacaatgttg
    14281 taagcatgat gaaatctaga ttaaattcta atgcagttcc tatgcaatta ccaataggta
    14341 aagaagattc atttataggt ataatagact tattaaaaat ggatgctgtt atatacaaag
    14401 acgacctagg ggttgaaatg gaagaaacag atataccaga agatatgaaa gaattagctg
    14461 ctgagtggag agaaaaatta gttgaatcag tagctgaaac tgatgaagaa ttaatgatga
    14521 aataccttga aggtgaagaa cttactatag atgaattaaa agttgctata agaaaagcta
    14581 ctatagcttg tgaaatgaac ccagttttct gtggtacagc ttatagaaac aaaggtgttc
    14641 agttagtaat agatgcagtt ttagattact taccagcgcc aactgatata ccagctataa
    14701 aaggtatatt agcagatgga gaagaagctg aaagacattc ttctgatgaa gagccattct
    14761 ctgcattagc attcaaaata atgactgacc cattcgttgg aaaactagcg ttcttcagag
    14821 tttattctgg aacattagaa tcaggttctt atgttttaaa tgcaactaag aataagagag
    14881 aaagaattgg tcgtatatta caaatgcatg ctaatacaag agaagaaata actaaagtgt
    14941 atgctggaga tatagcagca gcagtaggat taaaagatac tactactgga gacacattat
    15001 gtgaccctgc taatccaata atacttgaat ctatggaatt ccctgagcca gttatatctg
    15061 ttgctataga accaagttct aaagcagctc aagaaaaaat gggtatagct cttcaaaaat
    15121 tagctgaaga agacccaact ttcacagtta agactgacca agaaacagga caaactataa
    15181 tatctggtat gggtgaatta cacttagaga taatagtaga tagattatta agagaattta
    15241 aagtagaagc taaagttggt gcaccacaag ttgcatatag agagactata actcaaccag
    15301 ttgatgttga atacaagtac tctaagcaat caggtggtag aggacaatac ggtcacgtta
    15361 agattagagt tgctcctcaa gagccaggtg aaggatacaa attcacaaac aaaactgttg
    15421 gtggatcagt accaaaagaa tatgttggtc cagttgacat gggtatacaa ggagctatgc
    15481 aatctggtat agttgctgga tatccagttg tagacgttgc tgttgagtta tatgatggtt
    15541 cttaccatga ggttgactct tctgagatgg cgtttaagat ggctggttca atggcattca
    15601 aagatgctat gaagaaaggt aatgcagttc ttttagagcc ttacttcaaa gtagaagttg
    15661 ttactccaga agattacatg ggagatgtta tgggtgactt aaactcaaga agaggattaa
    15721 tccaaggtat ggaagctaga agtggtgcac aagttataaa tgcattcgtt ccactttcag
    15781 aaatgtttgg atactcaaca gacctaagat cttcaactca aggtcgtgca acttacacaa
    15841 tgatattcga tcactatgag caagttccag cttcagttgc taagaaaata gctgaaggaa
    15901 aataatttaa aaataattaa taatataaat ataaaaaata atcagctaga tgagagatat
    15961 ctctcatcta gcacaagat
//
LOCUS       DAEMHZ010000096        15823 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000096 DAEMHZ010000000
VERSION     DAEMHZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.096"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(122..685)
                     /locus_tag="KPK72_002681"
     CDS             complement(122..685)
                     /locus_tag="KPK72_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2489882.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(783..1712)
                     /gene="glsA"
                     /locus_tag="KPK72_002682"
     CDS             complement(783..1712)
                     /gene="glsA"
                     /locus_tag="KPK72_002682"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG2489883.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(2309..3976)
                     /locus_tag="KPK72_002683"
     CDS             complement(2309..3976)
                     /locus_tag="KPK72_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG2489884.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(4050..4805)
                     /locus_tag="KPK72_002684"
     CDS             complement(4050..4805)
                     /locus_tag="KPK72_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG2489885.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYICIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(4815..5351)
                     /gene="lepB"
                     /locus_tag="KPK72_002685"
     CDS             complement(4815..5351)
                     /gene="lepB"
                     /locus_tag="KPK72_002685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2489886.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLKENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(5517..6053)
                     /gene="lepB"
                     /locus_tag="KPK72_002686"
     CDS             complement(5517..6053)
                     /gene="lepB"
                     /locus_tag="KPK72_002686"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2489887.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(6153..7463)
                     /locus_tag="KPK72_002687"
     CDS             complement(6153..7463)
                     /locus_tag="KPK72_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG2489888.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENSSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(7542..8258)
                     /locus_tag="KPK72_002688"
     CDS             complement(7542..8258)
                     /locus_tag="KPK72_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG2489889.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            8503..9774
                     /gene="sleC"
                     /locus_tag="KPK72_002689"
     CDS             8503..9774
                     /gene="sleC"
                     /locus_tag="KPK72_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG2489890.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(10241..10897)
                     /locus_tag="KPK72_002690"
     CDS             complement(10241..10897)
                     /locus_tag="KPK72_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489891.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVTV
                     "
     gene            complement(12170..12580)
                     /locus_tag="KPK72_002691"
     CDS             complement(12170..12580)
                     /locus_tag="KPK72_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489892.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(12796..14178)
                     /locus_tag="KPK72_002692"
     CDS             complement(12796..14178)
                     /locus_tag="KPK72_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2489893.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEEMYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(14189..14548)
                     /locus_tag="KPK72_002693"
     CDS             complement(14189..14548)
                     /locus_tag="KPK72_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2489894.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(14753..15532)
                     /locus_tag="KPK72_002694"
     CDS             complement(14753..15532)
                     /locus_tag="KPK72_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG2489895.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         5376 a         2878 c         1530 g         6039 t
ORIGIN      
        1 caaaaaaata gagattaaaa atcattgttt tacccatacc aaaaactatt ttttgattat
       61 taagtaaatt ttgatgttca atctctaata ttatatcttt aattaatcca taattaattc
      121 tctaactagg acattcctcc tcaagagtag gaactataac catagctata tcatacttag
      181 aaccttcaac attgtgttgt cttttattta aaatttcatc tatctttgac ttcatagtgt
      241 cactaagttc aactaattca tcatgagtaa gagaaatatc atgataatca actatatttt
      301 cattttctaa aacatcttcc tctgctgcct tataaaaaac ttcactcata ttttcaaatt
      361 ttgatatgta caattcttct ttttcttccc cattgtctaa agaaaaatta agtatcctat
      421 tccctataac cacatttttt gcacttggat aatagtactt ttccacaatc cccgacttta
      481 ttttttcctc aactaattct aaaataccaa ctttgtataa catttttata tggtagttta
      541 tttttgcatg aggttcttcc aataattgag ataattgttt tgctgacaat ggcgatttgt
      601 caaatgcttt tagaatatct attctacgtg gatgcgcaat agcctttatg cactccaaat
      661 ctcgtaatac caatacctct ttcattgacc cctcctaaaa aatttattta attcatattt
      721 ctttacaatt taattatata ttaaattata tgttgttgtc aaaaaaatcc tttcttaata
      781 ttttaaaaaa tatttaattt gagtttattt gacaactcct ctaaaacttt aactcctgcg
      841 acgctgttac cttttttgtc aagcgcaggt ccataaacac caatccccat gcgtcccggc
      901 acagaagcca ttataccacc cccaactcca gattttgcag gaatgccaac tttaatagca
      961 aattccccag aagcatcata cattccacaa gtcatcataa atgtcttgac aatcctagaa
     1021 accatttcac tcattaaatg ctcaccattt tctatatcta ctccataatt tgcaagattt
     1081 attccaattc tcgctaaatc tacagaatct atctcaattg agcattgttt aaaataaagg
     1141 tctagaacat cttctacatt ccctctgata aatccatcac ttttcaataa gtaagccata
     1201 gctctgtttc tatctccagt cattttttca gacttataga catcatagtt tattcctata
     1261 ttatcatttt ttgctaactt tctaaagaaa ctaagcattc tttcttcttt ttcttctaaa
     1321 caactcccat ttataaggga agtagttact atagcccctg catttatcat tggattacag
     1381 ggtttttttg tgtcattaat ttcaagtttc attattgaat taaatgggtc cccacttggc
     1441 tccattccaa cgtttgaaaa aacgtcttcc caatcattat ccataagtgc catagcaagc
     1501 acaattggtt ttgaaatact ttgtatagta aactttgtgc tacagtttcc agcactataa
     1561 agattatttt cgctatctat gatacaaatt cctaaatcat ttcttctagc gttttttaat
     1621 tctggtatgt aactagcgac ttgtccataa ttagtatact tcttattaga acttataatt
     1681 tcttttaata atgtctcatc taacgtattc atatgcacct cttatattat gttatatatt
     1741 ctattttagc acaagtttat actataataa aacaatgtta aaaaaaatta tactttaaaa
     1801 ttattttcta caagaatcaa ttcatttttc aaacaattta aatagtttaa agagcttaat
     1861 aatttagtaa ttcaataatt taacaaccta gtaatctaac tatctaataa tccagtaact
     1921 taacaaccta gtaatttagt aattcaacaa cctaataatt caataattta acaacctagt
     1981 aatttagcaa tccagcaatc taataatcca gtaatttaaa tttttataac accaaaaatt
     2041 tctaacccaa caccttttac tcttttactc ttttactctt ttaaaaaata atcacataaa
     2101 ctaacaaaat attaaacaaa aaagtaaaat ttaaaataaa aagcttgctt catacaaaag
     2161 tataaaacaa gcctttttat atatcaactc aaactttaaa atctgtatca atttaattct
     2221 ccactttaca cttaatatac ttaacataaa ccaataacac tacttgctat gccatttaat
     2281 cttcataaaa caatctataa tacacacatt aatcaactat tttacttcct ccaataaatt
     2341 ccctaagaat tgaaatacct ggtatgtcag aagtatcgtc caattcaaca aaatactgca
     2401 aaaactttag taatctagta gcttcaatat gtgcaggatt atcttccttt atttcttcca
     2461 acaaaggttt cgcaaatcgt tttaaaactg ccaattcata tacacaagca gagttaaata
     2521 tagcatctgc ctcctcttgg tacgggaata tattcttctt ttctcctctt ctaactgatg
     2581 cccattgtaa aatagtatta tcagcactat atcctctaaa attatagtct ctaacaattc
     2641 ttcttatcat tctcaaatca gcagtaggga ttcgattatg gtcatccaaa tttatttgag
     2701 taagggcact tatgtatatc ttaaatttat cttcatctgg tatagatgat gttaaaatag
     2761 gatttagccc atgtatacct tctaatataa ttggctgatt tgaatctatc tttatctttt
     2821 taccagtgta ttctctctta ccttctttaa aattgaatct tatatcatca atttcttcac
     2881 cagctaaaag tttctttaag tctaagttaa acttctctaa atctattgca taaattgatt
     2941 caaaatcata atttccatat tcatctaaag gagtatgttt tctatctata aaataatcat
     3001 ctaaagaaat tgaaatagga tttaaattat ttactctaag atgtattgat aatctgtgag
     3061 caaaacttgt ttttccagat gaagatggag ctgctattaa aactactctc ttattgccat
     3121 cctttatcat atccgctatt tgggataact tcttttcatg caatgcttca aatgtcctta
     3181 ttatatctcc atattcattg ttttctatta ttttattaag agatactacc ttgtcaattc
     3241 ccataagtct tgaccattct tctgcttcat aatatacact tgatagctta ggttgaggta
     3301 caaatttcat aggaagagtc ttatcttctt cactaggtcc taaaattata acgcctccat
     3361 tataaagctt aatatcaaat gattttatat aaccagttga tggcaacata taaccataaa
     3421 aatggtctat atagtttcta cacttatata ctttagcact atcatactct ttgtatttca
     3481 aaagctctgc cttttcatac atttcattat tttcaaagat agaaatagct tcatttcttg
     3541 taacttcaat tttttctata acataatcgt tgtttactat ctctttcatc ttgtttttta
     3601 tattttcaac atctgcttct ttaagctttc tgtctatatg aacttcacaa tatagcccat
     3661 ctgatagtga atgttctatt gtaactctgc tatcccaaaa gatttctcta catgccatta
     3721 caaatataaa tgacatgact ctaaagtaaa ctctctctcc atcttcatta gtagtatcta
     3781 aaaaatttat ctcacaatcc ttcttaactc tacaatttag ctcttttagt ttattgtcta
     3841 caactgcaag tgttatataa cccttatgct catcacaaaa ttcttttgct atatctccca
     3901 atctgattcc aggggaattt tcttctaatg aatattcctt atcttttcct tctattttta
     3961 agtttatttt tttcacttca acatctcctt ccttttgatg aatataaatt ttaattaatg
     4021 aggcatatca acaagataaa gagaggtatt taaccctctc ctacaatatt attcaattta
     4081 cttatagtat cttcaatgtc ttcgtaattt atttctaata ctaaatattc tgcatctaat
     4141 gttaagacct ctttaatccc ctcttcagat aaaattcctc ttcctatacc aacatgtgta
     4201 tcattgatac catcattatc acttaagtgt atcagtctta ttttatcctt taagttcttt
     4261 acataagcat cctcatcaat taaacagtgt ccagtatcgt aacaaaaaca aatcttagaa
     4321 tttttaatcc tcttaaatat atattcaaaa tcataagata tatttccaat atttgaaaaa
     4381 tccccacctt ttgaataagt attctctata catatatatg aatttgtatt taatttatct
     4441 aaaaaatcca cactatttcc taaatatttg tctctattct ttctaactct atttgtcata
     4501 acatatccca aatgaaggtt aaaatacctt aaatcaaatc cttttagttc aaattctatc
     4561 ttctcaataa aatttatcca tgaatttcgt atatactcta tgttttcgca agtatttaat
     4621 tccataggca aatgtatccc tatagataat cctaacttag aaattctctc tttatattta
     4681 cataactcac tacattcaga tatattgtca attccaatct ctaaatgagt tatttgcttt
     4741 atactctcac aaatgtttaa agcctcctca atattaaaca ataaggacgc tataccaact
     4801 gccatacaaa tcacctactc aaaaatacct atatctgtaa aaggaaatac tcttaaaata
     4861 acttttcctt taatattttc ttcatctact aatcccactt ctttatatct actgtctaaa
     4921 ctaacttctc tattgtctcc catcgcaaat acttttccct taggaatcac catatcaata
     4981 tcgccttcag tataattatc atgtatatat gaaacttcat ttaataactt accatttata
     5041 taaaccttag aatccttaat ttttagatgg tcaccaggaa cacctataac cctttttaca
     5101 agctcttttt tccttccatt ctcttttaat aaatcagtct taaatatcat tatatctcca
     5161 cattttggtt ctgataactt atatgtcatc ctgtttgcta ccaaataatc atgtgattta
     5221 agtgttgggt acattgattc tccatttact aatgttggtt ttataaaacg agttataata
     5281 aatgcaaaaa aaagtgcagt aataattatt tttatccact ctacaatctc ttctttaata
     5341 gtttcattca ttcacaaatt ctcctttata gttatattat ctttattgat aatgtctctt
     5401 aaacactatc ttttagataa gtctactaaa taaggtgttg tcttttttat atagacaact
     5461 attgtgtatg gtctggtgat ttcagctaat cagcgattgc tccaaatttt atatgtttag
     5521 tcaatagttc ctatcttaga aaatggatac aatctgacta gaacctttcc taaaatggta
     5581 tgctcgtcga ccaatccaac ctcatcgtat ctactatcta aacttttttc tctattatct
     5641 cccattgcaa ataattttcc ttctggaact acgatatcaa tatctccatc agtacgatta
     5701 ttatgtatat aggaagtctc atctaacaac ttatcattta catatacctt ggagtcttgt
     5761 atttttacat ggtcaccagg aaccccgata actcttttta ctaaatcttt tttctttcca
     5821 ttttcctctg ttaagtcggt tttgaatact attatatctt tgtattttgg ctctcctacc
     5881 ttgtacgcaa ttctgttaac tatcaaataa tcacgttcaa ctaatgtagg atacattgat
     5941 tctcctttga ctattgttgg cactataaaa cgagttattg caaatgccaa aacaagagct
     6001 atgacaatca cttttatcca ttctacaact tcttttttaa ctgcttcacc cactaaaatc
     6061 gtctcctctg tgttaaatat taagtcaatc tacagcaaaa taatctacca taaacctaca
     6121 atataaatat tatacttctt ttttctaata gtttaaaata cttttctaag acctttagga
     6181 taatgattct taattacacc agaagattct tttccccaac ctaaaggtat tccttcaacc
     6241 gaaaccaaaa cccatcctct actatcatta gaatttacaa catccccttt taaatattta
     6301 agaattgatt cactctgtac actggagttt tcaacatttt ttacatcttc aacttttaag
     6361 taatgagata gtgcatgaga aggctcaaat ctattcttct ttaaaacccc taaatgcaaa
     6421 ccatatctca atacttttag ttttttcgta tctggatata tttcaggaag taggtacaaa
     6481 ttttctcctc ttaaataaaa tttatttttt ttattactat aaaaatcaac ctttaaaaat
     6541 ttcttttcaa actccctata atcctttatt tctttatcta aattttttat ttttaattct
     6601 ttgacattac attcttcttc ttctaatttt tttattttgg caacaaaatg accttctccc
     6661 tttatcttat gaggccataa tctttccatt tcaataagtt ctgcatgtgg atattcactg
     6721 ataaactcat ttataacatg ctcattttcc tcccttgaaa aagtacaagt tgagtaaatt
     6781 aaaatgccat ccttctttaa cattttatat ccatctctta taatctcttt ttggatggat
     6841 tgacattcta ctacctttgc ataactccaa tctgatatgg caacctcatc cttcctaaac
     6901 ataccttgcc cagaacaagg tgcatctata ataaccttat caaaataccc attaaacgtc
     6961 tttcttaagc tattagaatc ggtgtttgtt ataatacaat ttctcgctcc aaatcgctct
     7021 aaattttccc ctagagcctt aattctacta gcattaattt cattagaaac caataaacct
     7081 gtatcgttta gcttagatag tatatatgta gattttccac caggagcagc acacatatcg
     7141 agaacttttt caccctcttt aatatctgcc tttggtatta cactcattgc agaaggctct
     7201 tgcaaatagt atgctccagc ctcatgcagt ggatttctac caggtctgtc aattttatca
     7261 tcataataaa aaccttcttc tgcccatggt atttgattca aatcaaacaa ctccaaatca
     7321 aataattttt tcttgtcaat ttttaaagta tttattctaa gcccagtagt cttactttcc
     7381 tcataacttt ttataaaatc atcatattca tcttttagta tttcttcaat atcttctaaa
     7441 aattcctttg gtaattctgt caaatctata atcccccttc aatctgcttt acttgtgtgt
     7501 catttattat actttaatta tttatgtctt tcctttaaat tttatttctt cttctttttc
     7561 ctaagattac cgaaaaacac ttttttagag tgattcatat atttctcaag tttatcaaga
     7621 tttatcttcc acaaaattat tacataaact ataagtaata taatagccca tctatattct
     7681 atcatcaata tgaaatcaaa aactccatgt aataaaattg gaattaacac tgccataaac
     7741 aaatactcac gtcttttatt tttatttccc tcaaatttat acttagaaat atagtagccc
     7801 atagtaattg caaacattac atgagctggt atagagataa ctgccctatt aataccaact
     7861 tgcagtgcta gatttctact ttcagaaaaa atataaacca tattttcaat tgttgcaaat
     7921 cctaaagcta aaaatacaga gtaaataata ccatctaact tttcagtaaa gtgtttttct
     7981 ttaattaggg caggaatcag tatcagagct tttacacctt cttctacaag tcctgcaact
     8041 acaaatgata catatattag ttctgaatac ccttcaaata cattaaactt catcagtaaa
     8101 ttttctaaaa gtataattat aacacttaga aaagcaccta ttaaaaaaaa tttaattaga
     8161 tggattaggg gttctctaca atatctatct ttagacctta tccaaaacat ccctatcaat
     8221 ataggaacta ttgctaataa aaataaatct agcttcatag attattttcc tttcaaaatt
     8281 tttgatttat ttatgattta tatcatctac ttatttttaa cttattcgtg ttttaaatac
     8341 atactaaaat atacattaaa ttattttaaa ttatttaaat acaaatatac tcaaatactc
     8401 aaaaaaattt aaatttatat ctatattatt taacaaattt caagctatat tcatatcttg
     8461 aaatataagg gaactaaatt catttaaaga aagggtgatt tgatgcaaga tggtttctta
     8521 acagtaagca taattgatgc tactaacaat agacctattc aaaatgcagt agtaaacata
     8581 tattcaatgt ctaatggtag ccaaagctca tcaactttat accaaaacct aagaagtaat
     8641 gaatctggtc aagtgactgg tttagtatta ccagcaccag atgtagatta ctctctacaa
     8701 ccatctgatg ttagacctta tagccagtat atagtggaag ccatagcaga tggatatgag
     8761 acagttgtca tagaaggaac acagttgtta gctacaattg aagcaagaca aggagttccc
     8821 atgtctccta gaacaagaag taagcgttct ttctcaagac aaagtgagtt gatttttgac
     8881 ataggagaac atactctata tggtacttat cctcctaaga ttccagaaag taacttaaaa
     8941 ccattaccac ctcctactgg tttcgttgta ttagataacc ctgtagtgcc tgaatttata
     9001 gtagttcatg atggtttgcc agaagattca tcagctccaa actattggat tccatttaaa
     9061 gagtacatta aaaacatagc gtcatctgaa atatattcaa cttggcctga acagacaatt
     9121 tatgcaaatg taatagctat aatatcattt actttaaata gagtctttac tgaatggtat
     9181 agaaataaag gatataactt tacaataact tcaacaacag catacgacca taagtttata
     9241 aataatagaa acttatttga accaattaac gtagttgtag atgctatttt taatactttt
     9301 ataaaaagac ctcctacatc aagacagcca ctattagcac aatattgtga tggacaaaaa
     9361 tcccaatgtc ctgaccaaat gactcaatgg ggcagtaaag acttaggtga ccaaggatat
     9421 gactatgaga gtatactaag atatttctat ggagatgaaa tagtatttga aagagcacct
     9481 atagtaagtg gtgtaccagt gtcattccct ggtactacac tacaagttgg ttcttctggg
     9541 cagtatgtta gaaccataca aaatcagtta aatgctatat ctaattctta tccagctgta
     9601 ccaaaagtta tagaagatgg tatctatgga acagatacag aaaacgctgt taagatattc
     9661 caaggaatct ttggtttacc tcaaagcgga gtagtagatt ttaaaacatg gtatgaaata
     9721 tccagagttt atgtagctac aactagaata gcttctttaa atcctttaat ttaaagcttg
     9781 atttagaaca taaactcaaa ttattgaata aaattattaa tttaaaataa aaatggctgt
     9841 tatgaactaa tatataccat aagtattact tataagtatt gtagtcttaa cagccatttt
     9901 ttagttataa cttgtttttt tagaatctta aaactttaaa tgggggattt taagcttctt
     9961 aaaaacagtg cttagcaact tagatttctt taaaatatta tatttatttt taagtttaat
    10021 aaaatgctat tctaacgaaa aacctacttt ttatttaaaa tctcacttat tatttaaaac
    10081 actatctatc acttagaata ttacttctta ttatttaaaa cactatccat cacttagaat
    10141 attacttctt atataaatta ttatttatta taaatacaaa taatattaat ttaaaaagtt
    10201 attcctaatt taaataatac tattatcttc atcagtgaat ctaaactgta actttagact
    10261 tattagctaa agccaatatt agacctaaag ctatagatgc aaatgcagaa gcaattactc
    10321 tatactcagc agggaataag aatacaattg tatgacatgg tatccagaaa aatggtatag
    10381 tttttaaaac actaaaacca aggtaagcat tccagtccac aatatttacc acatctttaa
    10441 gttttacctt aatccccttt ccaacctcat attttttatc tataaaacag tccataacct
    10501 tatggaataa aaatacagtt ggtgacatta gtatattcat agttgcactc ttaaatagag
    10561 catttgcaaa agctattccc tcaaatggaa gcataccatc cacttgtgct tgacctacac
    10621 ctgatgagta gattgacatt ccatatgtgg taactacacc tattattccc caaactattg
    10681 ctctataaaa tactcctact ggtattttcc aatccttata caaaattcta gcaccaagta
    10741 aatctcccat tgtagctaat acagcaaact ttaaaaaacc tccaatatat ggatgtgcac
    10801 ctgttgcttc cataaatact tctctactac ttggtattgc taagacagca ataatacaaa
    10861 taagaactcc actccatata aaatcgcctt ttttcatatt tataaatact atttaatata
    10921 taaatagttc tcctttctat taaagattta tttaatttat aatctatcta caattattta
    10981 ttaatcactt attattagat attcattttc catatttaaa tttattctat ccctttatct
    11041 atttattttc ttactactct atccaccatt atctatatat ttattttacg attactcttt
    11101 caccctaacc ctttaattat ctattttttt ctgatattct tatctaccat catccatata
    11161 tttattttac gattactctt tcaccctaac cctttaatta tctatttttt ctgatattct
    11221 tatctaccat tatctatata tttattttac gattactctt tcaccctaac cctttaacta
    11281 tctatttttt cttactattc ttatccacat catccatata tttattttac gattactctt
    11341 tcactctaac cctttaacta tctatttttt ctgatattct tatccaccat catccataca
    11401 tttattttac gattactctt tcaccctaac cctttaacta tctatttttt tctggtattc
    11461 ttatctacta ttatctgtat atttatttta tgattactct tttaacctac cccctatact
    11521 ttatagtaat atgtttttaa cctttttcat tattcccaat atgctttata actacaaata
    11581 ctctcccccc ttaaaaataa taataaaatt aatcaataaa tttataatca tattattact
    11641 ctatgtatta agcaattact atgccaataa actttgattt ttttaaacaa atcttaaaaa
    11701 tcctaaaatg ctaattttct ttaactttta atgaatctaa tagataacat ttaattttcg
    11761 ttatattttt cagaatattt ctattattat ttttaagaaa aaaaaataat gttaacattt
    11821 cttaacatac attaaaaaat gttaacatta taaattgggt ttaatacttt tactttgact
    11881 cgatttactt ttattctact tcttacttta tttttaactt agactttatt tttttattac
    11941 ttatttgagt cttatttatt ttattatcac tccatatgtt caattacatt tgtctaaatt
    12001 ctttgatact tttattacat aattttaaca agcttaagtg ttatttgggt agtgcagttg
    12061 ataattattt aaataatcat caactgcatg tttatatcat tacagtagct taatgtattt
    12121 aatcatactt acttaggatt ttgaaatatc ttagagatta tatttaacat tagtcaattg
    12181 ctgcataatt gtaagcaatt gaaatttgag acccatcttc attttctata gtgataggct
    12241 tcataatttt taatccttca gtttttatct tttctaattc ttgttccatt ttcttaattg
    12301 tttcctcaag gtctttttga cttacatctt tgactgcatt agggtcttta gcatagtctt
    12361 ttaattcttg aatatgttta gcatactctt cataagtata atgctcaaca ccatactttt
    12421 ccaattcagt agggtcagtt attcctgatt ttattagaga ttcaagttta ggtaaagcaa
    12481 tattagatga atcatttcca tttgcaaata ctgctccagt tcctcctagt accattgcag
    12541 ataccatcat tgcacttata agggattttt ttattttcat aaccaatctt cctttcttag
    12601 agatttattt gggtagtgta gtttaaaatg actattgcaa tagttatcaa ccacatcctt
    12661 agactatcat agtagtcatg tttatgtcaa gcctttttaa aaaacttttc aataaaaaat
    12721 actatctcaa aacaaaatta attattttga gatagtattt taattctaaa tcttttatat
    12781 tactaagatt tatggttact ctatccatgt tctatattgt tctttattaa ctgctaacaa
    12841 attccctttt cttgatatga caaagcgatt atcattatag ccacttcgct caattgaaac
    12901 ttctcctgaa tcagtataaa gtacatcctt actattacta gatttattta catacatttc
    12961 ttcactattc tcagaattag ttactttgct catagatgat tcttcatgct ctctaaattc
    13021 aattacagat actttcgccg tactaaaatg agtctctcca tcacttttag ctttactaac
    13081 aagaatatca gattctactt tgggagaata taaatcagtt gatttactac ttattgaaaa
    13141 tccaatttta tcactttgtt cattttttgt taaaccaaaa gattcaacct cattaatctc
    13201 tttattttct gttgcaaaaa caagacctgt cccacaaact aatacagttg aaactaaaaa
    13261 agtaacataa gttgattttt taaatttcat aatattaact attctctttt ctatttttgt
    13321 ctttccaaat ccactacata ttgtcattga aaatggatta ttctcctctg ctagacttat
    13381 tagtgtggtt gcataatcag ccctatattc ttcaccaagt atactcataa ccttttcatc
    13441 acaactaaat tctatgtctt tatttagtaa aaaaaacatc acccaaacta agggattaaa
    13501 ccaatggaca acaagagcta caagcataaa taactttaac agattatcaa agctattaat
    13561 atgaaccatt tcatgagtta aaacaaagtc taaagtttga ttattagcat aattcatttg
    13621 ctttggcaaa ataattcgtg gttttaaaat accagatgta agtggtgtcc tagtaaattc
    13681 agatatgcaa acaataaatc ttctaaaacg gctattctta cacatccatt ttttaatgaa
    13741 ctcatgattt tttatatgaa ttgaagtttt aaaaagatta attattttaa aatatctaaa
    13801 gcataaaagt atgctaaaca taatagtaac tataaaagac aaaatgccca acatttccaa
    13861 catcataata ttttcaatat tagacattat ttcaaattcg tcagaattgt ttttacctgt
    13921 agagtcacta gctttcttaa gagtataatt ttctgttgat gtattaccca attccacatc
    13981 aaaagtcgat tcaattatac tataaatact aaaaccagaa tttaaactta ctggtattag
    14041 aagtttaaat aaaacaatca accacaataa tacaaatacc tttttaggta attttttaaa
    14101 taaacatgct cttataaaaa taactattaa aagtaatact ccagaagtaa aactcatttc
    14161 taacaattta atgaacataa atcttacctc actttaattt ttcaacaatt tgtttaagtt
    14221 ttgtaatctc atcacttgaa agattttctt tattaataaa agctgaaaga aatttttctg
    14281 aagaccctcc aaacatttta ttaattaaag tttctgtttc ctgtttttgt acttcctctt
    14341 tgcttattaa agctttacaa acaaaattcg gctcaattct ctctattgca ccttttgaca
    14401 ctaatttttt tataacggta tacgtagtgt ttctattcca tcctgtttct tcttttaaaa
    14461 tttttactaa ctctccagcc ttgctttctc ctctctccca tagaacttcc attactttaa
    14521 gttctgaatc aaataatttt atctccatat aaaatcgctc ctcaaatata gttatcacaa
    14581 tagtcattac tataataaaa aactatcata gtagtctata ctatgtcaat catttatttt
    14641 ttaataataa aaactgtctt tttaaaaaat tcttataaaa gacagtttta tcaatgttat
    14701 actttaatgt ttaattaaag ccatatttta tttaaatata taactatcta tcttagtaca
    14761 tagtataaga tttttcttta tctaatagta tatcctctac atctgggttt tcttttacta
    14821 attgtctata aaaattacaa ttagtaacac ccacatatgg aattaaccat agtagaccta
    14881 ttccaagagt agctattgaa agtatatacc atcctataaa actaaagttt agtacaaata
    14941 atttaagtac atttcctttc atcatatcta aactagcacc cattgattta aatacaccca
    15001 tattatcctt gtcttcacaa attaagaaag tagcttgaga aacatataat agcaatatat
    15061 aaactggtaa cataagaaca agtattataa gaactagagt tattccagta gaaataatag
    15121 tccctattcc aacattatca cttgataaat attcaattgc tccactagta agtaaaactc
    15181 caaatgataa aaacgctcct ataattgtga ttataaaaga ataaatataa acaataattg
    15241 attcaaataa ataaaaacca aaacttctta aataaattct cccacttaca aagatatctc
    15301 ctatcttgac tttttcatct ttagtaattt tcaagaacat acatgtaaat cctgctgaaa
    15361 cgcaaaaagc tattacagcc actacaatag tagtcaatgg ttcaaaaggt aatcctacaa
    15421 ttgatattaa tactatcaat gcaatatata ctgccataga aattgcacat atcccccagt
    15481 ttccagataa ctgagcttta gatactcgtt ttaattcttg ccttgatacc ataaaaaaca
    15541 cccctttcat tttataaaaa aacttttaaa aatataaata atcttttaac gtataatatt
    15601 aatatatcat attttcgact atataagata caattattca ccatgtttta tcaagatatg
    15661 ccataattgt attactagtt ttatatagtc aacattacca aatacatcaa ataaatttga
    15721 ttaattattc aacaagtaat aaaataattt ctattaatta tactttattt agtaaacaaa
    15781 ttatttaata aaaaaattac ctaatacagt aaattaattg gta
//
LOCUS       DAEMHZ010000097        15815 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000097 DAEMHZ010000000
VERSION     DAEMHZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.097"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..583
                     /locus_tag="KPK72_002695"
     CDS             <1..583
                     /locus_tag="KPK72_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489896.1"
                     /translation="SEIINKLFFNELKELIDKCNNIEDKTVYMIEKIFISIEDEEIKN
                     YLIRNNCKLQELVNRYKKLELLNIDEAIFFAWYNLNINTISIDKASQYYYELTTSNYI
                     EVESHLVYTNEIDLREYAKDELDTMLDMEYHIDRLLDKDMIIDMWLNNTSKEDVIEEI
                     LMSDDIEDILDISPEYAFTTTSGIEYRYSEKEE"
     gene            779..994
                     /locus_tag="KPK72_002696"
     CDS             779..994
                     /locus_tag="KPK72_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBG2489897.1"
                     /translation="MVDILGEEIVIKLYKYYRGQQITFPMKLYSNEYVERYIEKNYRT
                     KTLKDMCRELGYTEGWIKQLINKYKLK"
     gene            1223..2749
                     /locus_tag="KPK72_002697"
                     /pseudo
     CDS             1223..2749
                     /locus_tag="KPK72_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3043..3381
                     /locus_tag="KPK72_002698"
     CDS             3043..3381
                     /locus_tag="KPK72_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489898.1"
                     /translation="MEQYSFSSELIDVMVNASNDLNRVYEKITSSGTFSGLQGTFDTL
                     YEYGLMLEDLLEVMDLSLLGGNETKEIIQNIAIDLKNISDEDNIADKALDLMGNIGEF
                     TYYIETNYSK"
     gene            3516..3761
                     /locus_tag="KPK72_002699"
     CDS             3516..3761
                     /locus_tag="KPK72_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489899.1"
                     /translation="MRSVTVEDGEERFAIEKIYVKSLKRYEVRICLYRDCKDRMNKLI
                     PRPVDISPDKLSTLILVGIKAGILDDDFKQELIKGLS"
     gene            4059..4250
                     /locus_tag="KPK72_002700"
     CDS             4059..4250
                     /locus_tag="KPK72_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489900.1"
                     /translation="MKYTLKYGLGQLKFDVKIRLEDILRLSSAGEDNDEVRLAERSRE
                     INDIIGKLYLRPSETKKKI"
     gene            4339..5252
                     /locus_tag="KPK72_002701"
                     /pseudo
     CDS             4339..5252
                     /locus_tag="KPK72_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5433..5714
                     /locus_tag="KPK72_002702"
     CDS             5433..5714
                     /locus_tag="KPK72_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489901.1"
                     /translation="MELKTIRITFTVSSETSEDDLRKLCKDYMGDEYVVNDEGDFEIS
                     FYLNTVLGYNEVLYFMELIHMELEKSITLLGIRYKGIGGLQMKHILQEE"
     gene            5721..7382
                     /locus_tag="KPK72_002703"
     CDS             5721..7382
                     /locus_tag="KPK72_002703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489902.1"
                     /translation="MIRNCSKIIKNYGFKINSKNHVFDSNGNEICNFVPIVLGQYMIG
                     DGRDRRTVIVLMGISEDGNQLETINIDVSDIEHLKWVKNSWGFQYKVHSKMKNQFLYL
                     IEVLVINGTTKKLSSQIGWVKDNGKYAYLHTRGVIGESDKEVDIGDNLSNYFLPSEIV
                     DINRACKASLNLLEVADYEVTVPLLALVYLSPLLQAIKDVGKMPEFVVWLFGTTCSRK
                     TSLARVFLSHFGDFTNRLPATFNDTYASIEIKANAIKDSLNLIDDYAPKQTKKQKDAQ
                     DEIAEKAIRAYGDRIARGRVNSSIISQKQYIPQGMLLMTGENLIRGHSTVARLVALEL
                     DKDSVNLKLLKEMQDSVSLLGESMRGYIEWLLHGMNKDSSDIGGILIHNFDLYKEELE
                     KIEYTHTKGGHGRSIESCAWLLVGISSMLSYFEDKKVISEVEFNQYIQFAKDTMVNVL
                     IKNNNITKESSPIDEFLYTIKEAIDSNSIKISTLVDGNKLNDNDDDTYGYKDDKYFYF
                     HPDKTYSYVQEIQSKSGNYISLTKRGLIKLLREHSIIKVDSDGSP"
     gene            7517..7954
                     /locus_tag="KPK72_002704"
     CDS             7517..7954
                     /locus_tag="KPK72_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489903.1"
                     /translation="MTIRINLDGENILENVDNKFYGLRARVDFDLDLLDEDVIDIMDR
                     ILTLDVNPSRMFITKGRVDIFYQPQKEISIESKLGYMKLINEFIDFIESQEIVEMKFL
                     GWNLTGDKIVESQANDSNISFSKEIFNPDTVYLDGLIEGYVNL"
     gene            8061..8978
                     /locus_tag="KPK72_002705"
     CDS             8061..8978
                     /locus_tag="KPK72_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489904.1"
                     /translation="MNYITTQGFDTIKRDKIEIKRMVYALENEGDSYNVIEECICTLE
                     ENIDVNDDFVIEVENVFSFVIDRIMDFIKEKNLPKNKIEHVRAYIDVFENPTSNTKIV
                     KSIDIYEDYDKRYIDNKLDKLFKSMDNMIKPEFVPVIDGKFDIRGSKDLKEYNKKLML
                     DIVKQKTSEVNNGGIRINKLSAILKLGDKSIDGISKMMKDVANLKIKPKSVYISEDTV
                     VVMYICNKSCIVLNIEEYSKILSDFVDYINNIEDRDLYLYDSILYGDYKINCGVDDRD
                     IIDFNYQSFNSKVTLFAGHIGNEIIQAAL"
     gene            9008..9163
                     /locus_tag="KPK72_002706"
     CDS             9008..9163
                     /locus_tag="KPK72_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489905.1"
                     /translation="MTETYKISIGRKDLYSKDYKAVERIDYEKRYIVNISRSLVDILS
                     ILIEEGI"
     gene            9245..10783
                     /locus_tag="KPK72_002707"
                     /pseudo
     CDS             9245..10783
                     /locus_tag="KPK72_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680119.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            10916..11980
                     /locus_tag="KPK72_002708"
     CDS             10916..11980
                     /locus_tag="KPK72_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBG2489906.1"
                     /translation="MSKIEYYMNLPYDLSGSMSKNRFRNELLWGLKKMYELYKTNEDF
                     VVVFDYRCDIEVHLENKFEFYQVKTQNNSGTYTINKLTDKKKGSKSVVGNLYSLKYDI
                     NMNEIDDVYVAIVSNAPLNDGKKTYNNSHEVKIKTIDSSAIEKIKLSIKNDLNIDEDI
                     NLENLFFIRIGLDLIEPQKTLIGETAIFFEDVFGRECSKVKSLYNVLYEEIYSKSAYE
                     LDINTYENIVSKKGINRDRMDIILKSYANNTDKSVELAKYSINEWCRNNFSRRLSLIK
                     SLTTIVSKLSESSILKDIENCILSYIYNNIDKLQVSDKELIEIISEIVEEKRPIEVSK
                     TDIEALILLTLKKYEEGVYE"
     gene            11973..13646
                     /locus_tag="KPK72_002709"
     CDS             11973..13646
                     /locus_tag="KPK72_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489907.1"
                     /translation="MNSLFVNSLYIFEYESKMAKKIDFKHGINILTSDEKSGNDVGKS
                     VLLKSIYHTLGADSIFDHMWNKITKTYILNVTIKNKTYYVYRRDNLFKIYNTNFKKIF
                     MTNNRDELSNYLSDLYSFKVMLPKRENEELELAPPVYSYLLNYIDQDHLDGSKFSSFN
                     SLTQFPNYKENVIYTHFGLFDEEYYKAIKELEILKSEEKNKKKEREVTLNMLNKIKSY
                     LGNLDAPESIEVLNIELNKTKEEYSNIVINLQKIKNNLMKIRNEKVDLELNIKEIELT
                     RRNEMKKLKVKNVCPSCSHEIEDIDSKFIINNQIEDLIILKDELESEIIKCNRVIEKK
                     EEEYQSLLDKLHEYESNMKINDDSISNVLKHKAYIDTKDDIVLDLEIIDKKIKEIQQT
                     IEPYDKKIKEYNSLKKKANAKYEELMKKAKKDFGLEEIDENKIKNIKNNFIARGSNRA
                     ITTIMWYLTLLAVKYEFNENAIKFPLILDSPNNVESDEIKEESLFKYIFKNTKTDTQL
                     ILSSLGFNEMQFSEFKFDNIIKLENSKYNLLNKEDYYNNIDILNKIFEN"
     gene            13751..14713
                     /locus_tag="KPK72_002710"
     CDS             13751..14713
                     /locus_tag="KPK72_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489908.1"
                     /translation="MKDRFNLKRKSIQSLMNKNVDVYSCKDCIDDFIESNFKIYRSYY
                     ISDDDILEIMDGTGEDYKDVIADMLPDKAHIKSGEFGEITATEIYKSNIQTTEIYIVD
                     KLSHTRKEDTKVAAHKTDILIIEKTSEGFIIHSSEVKTKITDSDFNPIEAMIEGVNDD
                     IVTRVAETLNWIQRKLKTEKKFDELKFIKQVIESIENQNVENEYNGAVFIDESKLDYE
                     INKNIYCDVKLSKSQKGKYDNELKSIGCVIDGDKIRFDSVNIDDINKLNINSRKRNSI
                     EKLYNKSQRKLEHIDDIKIQIVTFDDINKHCLDMYEKIIHVGRI"
     gene            14713..>15815
                     /locus_tag="KPK72_002711"
     CDS             14713..>15815
                     /locus_tag="KPK72_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489909.1"
                     /translation="MSINNNINKIRNLDIQLAKYDCISRMSAKETSKHYMRLYFKFIK
                     LLELYEDSSLNINNGEYSNLANDLIIFSKYDMSSRFKGIDEERNLVYIAVIYYILGYR
                     FECNLILDILDMHDIENEYLKIIIYLIRNKYYKCDVLSCIKKYYIEDESNLSLIIKTL
                     YEKELVCDTKDQTYIQIIIAILRNIQNNGFYRVISKKSKLDINIWRKFVQQSIKYDQI
                     IYDLPKYHYDLLNDELLNIKHGYINMPTNYSKIEVIKIVIFNYLHTYQDKKIAYIHVD
                     KDDRAINAIKEYFTEYSTRIEFIEIDSINNYSEIATDNNFIIIDDFSNVSNKYYGTRF
                     EIMISLALKLEKRLLVLNNNYLKMDSNQLENIN"
BASE COUNT         6996 a         1278 c         2771 g         4770 t
ORIGIN      
        1 atctgaaata attaataagc tatttttcaa tgaattaaaa gaattaatag ataaatgcaa
       61 caacatagaa gataaaactg tatatatgat agaaaagata ttcataagta tagaagatga
      121 agaaataaag aattacctaa taagaaataa ttgtaagtta caagagttag taaatagata
      181 caaaaaactt gaacttttaa acatagatga agctatattc tttgcttggt acaacttaaa
      241 tataaataca atatctatag ataaagcaag tcaatattac tatgagttaa cgacaagtaa
      301 ctatatagaa gtagaaagtc atttagtata tacaaatgag atagatttaa gagaatatgc
      361 taaagatgaa ttagatacta tgttagatat ggaatatcac atagatagat tattagataa
      421 agatatgata atagatatgt ggttaaataa tacatcaaaa gaagatgtga tagaagaaat
      481 attaatgagt gatgatattg aagatatatt ggatatatct ccagagtatg cattcacaac
      541 aactagtgga atagagtata gatattcaga aaaagaagaa taaaataaat atatgtgttg
      601 aaaaaatacg ggctttaagg gcttatacct taaagtatgt ataaaaaata caatagattt
      661 tacaatagaa aatcagaatg aaactttttc taatactaat tattattaat gagtatcaga
      721 aaaggaggtg gagtaatgat caagaataat agcgactatc aaagggatat atgcagatat
      781 ggtagatatt ctaggtgaag aaatagtcat taaattatat aagtattatc gtggacaaca
      841 aataacattt cctatgaagt tatactctaa tgaatatgta gaaagatata tagaaaagaa
      901 ttatagaaca aaaacattaa aagatatgtg tagagagtta ggatatacgg aaggttggat
      961 aaagcaattg ataaataaat acaaattaaa ataaataccc tagttattgt taaattattc
     1021 aataaaaaat atgattttat tacaaaaatt tataatttcg acaagatgaa tgttgtatta
     1081 tatcaacgaa agatattaaa tttattcata tcaaatataa attatcaaac tttataacta
     1141 agttatctaa taagagacat tagtacaaag ttcattagta agtgaacata aagaattaag
     1201 gcaatggaga gggagattga aaatgacaaa gtttaatttt ataggaaatg aaatttatat
     1261 aacagaagaa agaaataggt ataatagcat aagaatagaa tatgaaaata tagcgaataa
     1321 agcaagaaaa gaatttatta aagtatatag gtcatgtaat gaaaatctag atgatgtaat
     1381 taataatgct tatgctcaag gggcatcaat tattttaaaa tcaattaagt gtacactaga
     1441 tcgacttata gaaaataaat tttataatat aagcgaagag ttatttattg aacaatactg
     1501 ccaaagagtt gtagaaattt gggaatctgc atacggtata atcaatgact agtatatgaa
     1561 tattgtatta gatgaaaggc aaaaggaaga gtatagacaa ctaaggaaaa attcaagagc
     1621 tagatggcaa ggtggaggtt ttgggattca aggggctata aaaggagcta cacaagcagg
     1681 agcaatgaat atggttacag gagttgcaca ttctgcattt aatatgataa gtaaaatagg
     1741 tacgagtata aaagtaaata aacaaaaatc taaaatattt aatgatccat cgacattaga
     1801 tgtattatct gaagtaattt atttagccat tttagatatt aaatactcat atataaagtt
     1861 tttagaaaat aatgcaggat tacagtttgg gtatatacat gaagaagagg aagaagaagc
     1921 aaatgtgata ctttcaaata taaaaggcag aaatttagat gaagaagaaa aaataaattt
     1981 aattaaggga cttatagatt taaatccata catggagtct ctatacacat acatagtaga
     2041 taactttggt gatgaaaata tggaagtttc taagatggct agttattttg gttttaatat
     2101 agagccatac aagcttagtt tagtagagaa taaattaatt aatttagaaa ctaacacaga
     2161 agaagaaaca atattagcta agcagcttat attaaaagaa gtaaatagat taggtattaa
     2221 tagtaaggta aatggaattg aagagttaga ccgaaaatta aatcaatttg atatagaagc
     2281 gaggacagta gataacatat tgtttgaaac aagagaaaaa gctaatttag caagacaaga
     2341 aaaattagat atagaaagta taataaaaaa tttagatgaa aacaatgaag aaaatctagt
     2401 taatattaaa aaagagattg aatctagaaa ttttaaaaca gaaatagccg atttatatat
     2461 agatagaata aataatcata ttaaaaattt atattctgac actataaatg aagcagaaca
     2521 atatgaggat aataaaacga attttaaaag tacgttaata ggatcagcgt ttattgtacc
     2581 tctaggtata tacttttttg gtaatgttgg gattatatta aagatagtta taggtatatt
     2641 cttattatcc gcagtatctg cattatttga atcatataaa aaattaaagg caagtaagta
     2701 ttcattaaaa caacttaaaa agttaaaaaa gtcaggtaaa ataatataat tatcatgagg
     2761 aatcaaattg aaaggtatat ttaataaaac attgacaaag attataataa gttcattaat
     2821 atttggaact gtatgtagta gtatagttta tctaggtatg agaaatgaat ttaacaataa
     2881 attagttttt tagaaaaaga gatactagac ttaaaaaaac aaaacgtaga attatctaat
     2941 aaagaattag aaaaagaata tgataattca gacgttaaat ctgaagatgt taaaaaagat
     3001 agagagatta ctaatgaggt aaaaaaagaa tatgaaaaag aaatggaaca gtatagtttt
     3061 agtagtgaat taatagatgt aatggtaaat gctagtaatg acttaaatag agtatatgaa
     3121 aaaataacaa gctcaggaac atttagtgga ttacaaggta catttgatac tttatatgaa
     3181 tatggattaa tgttagaaga tttactagag gtaatggatt tatctttatt aggaggtaat
     3241 gaaactaaag agattattca aaatatagct atagatttaa agaatatatc tgatgaagat
     3301 aatatagctg ataaagcatt agatttaatg ggtaatatag gggagtttac atactatatt
     3361 gagactaact atagtaaata attaaatatt aataataata gccttgctac acatgactgt
     3421 agcaaggtat tatttttgta aagaaaggaa gatttaattt ggctaaacaa aataaggctc
     3481 aactatttga aaaagaaact aaatattgta agataatgag aagcgtaaca gttgaagatg
     3541 gagaagaaag atttgcaata gaaaaaatat atgttaagtc attgaaaagg tatgaagtta
     3601 gaatatgttt ataccgtgat tgtaaggaca gaatgaataa gctaatacca agaccagttg
     3661 atataagccc tgataaatta tcaactttaa tattggtagg tataaaggcg ggtattttag
     3721 atgatgattt taagcaagag ctaataaaag gtttatctta ggaggtatag aatgtcgata
     3781 gaagaattta ctagtggagt aatgtttgga atttctatat ttggtacagt attaaagaag
     3841 ttattagata ataaaaaata gtacatagga ggtgttatta tgattggatt tataatagga
     3901 actatgatag gagttggatt atgcattagc atagaaatta tgataccaaa aaatacaata
     3961 gtatattaac ctatatttaa actgttaata taatcatata taatttattt gagtccttaa
     4021 agcccagaaa agttttatat gaaggagaat tttataatat gaaatataca ctaaaatatg
     4081 gattaggaca attaaaattc gatgtaaaaa taaggttaga agacatatta agattgtcat
     4141 ctgcaggaga ggataatgat gaagtaaggt tagctgaaag atcaagagaa attaatgata
     4201 taattggaaa gctatattta agaccgagtg aaactaagaa aaaaatctaa tagttatgaa
     4261 gaaagtaaaa ataaatctaa aagtaaatat gatagagata aaattaagga catattagaa
     4321 gaagatgagt tgaatgtaat ggaaaagtat gtattttcat ttgaatcagg aaataataag
     4381 ctatttaaag agtattggaa agacagaaag aagacagata taccattaat aacaacaata
     4441 ttttttagaa ctatgttaag attaaaggat acatatagac atgatttgta taacttatgg
     4501 gaagattgta atctaacaac ggaagaattt aaggaaaata tatataaatc acaaaatgaa
     4561 tctataggat ttattaaatt taaagttgaa aaaatgatag aagaagaaaa agaaaggtta
     4621 gggaaaagta ttaataacaa atataaaagc aatagaaaaa taacagaatt gcagaatatt
     4681 gatattgata atatagtaaa agaattgatt atatctgata atcctgaaaa taaagaaatc
     4741 tatcaaaaga tgataattat tacaaaatca ttttttaaga taattgaata tagaagaaaa
     4801 ttatcattaa gtaatcaaac agtaactgat aatttaatgg aagaggtaaa aaaactatat
     4861 aatacattac caaaagaatc gagatatgat gcagcatata agaatctaag aaagttaaaa
     4921 gaagatattg aatttaagat aaaagaaata gataattata aggagatata taaaagagaa
     4981 gttataaaaa attgtaaatt agttagtgag tataaaaaaa aagaagaaaa actaaagaag
     5041 tctggttata tagaagaatc agaaaaaatg gcaaaaaaat atatagatat agaaaggcaa
     5101 tttaatgaga gtatgagaaa aaaatggata tatatataga tatgtatata agacgtaagg
     5161 acttagaaat agaagaaatg gatatgaaaa aatatgaaga tgataatata acgaattcga
     5221 tgattaaatt agcagaagag attaactctt aattagcata aaaatagtac acagttaagt
     5281 gtgctatttt ttttatgaaa atgaaaaaaa attggataat ggtttggtta ttttttaata
     5341 aaaaattagc ttatatttaa tttcagaagg aggtaaggaa ataaaaatac gggctataag
     5401 ggctttagga caacttagta ggaggaacaa aaatggaact taaaacaata agaattacat
     5461 ttacagtaag tagtgaaaca agtgaagatg atttaagaaa gttatgtaaa gattacatgg
     5521 gtgatgagta tgttgttaat gatgaaggtg actttgaaat atccttttat ttaaatacag
     5581 tacttggata caatgaggtc ttatacttta tggagttgat tcacatggag ctagaaaaaa
     5641 gtattactct attaggaatt agatacaaag gaataggtgg actccaaatg aagcatattc
     5701 tacaggagga atagagtaag atgattagga attgtagtaa gataattaag aactatggtt
     5761 ttaaaataaa cagcaaaaat catgtatttg atagtaatgg aaatgagatt tgtaactttg
     5821 taccaattgt attaggacaa tacatgatag gtgatggaag agatcgaagg acagttattg
     5881 tattgatggg aatatcagag gatggtaatc aactagaaac tattaatata gatgttagcg
     5941 atatagagca tttgaaatgg gttaagaata gctggggatt tcaatataaa gtacattcta
     6001 aaatgaagaa tcaatttcta tacttaatag aagtattagt tataaatgga acaactaaga
     6061 agttaagtag tcaaattgga tgggttaagg ataatggaaa gtatgcatac cttcatacta
     6121 gaggtgtgat tggagaaagt gacaaagaag ttgatatagg tgataactta agcaattact
     6181 ttttaccaag tgaaatagta gatataaaca gagcgtgtaa agcaagtcta aatctattag
     6241 aggtagcaga ctatgaagtt actgtaccat tattagcatt agtatattta tcccctttac
     6301 ttcaagctat taaggatgta ggaaagatgc cagagtttgt agtatggttg tttggaacaa
     6361 cttgttcaag gaaaacttct ttagcaagag tatttctgag tcactttgga gattttacta
     6421 atagattacc tgcaacattt aatgacacct atgcatctat agagattaag gctaatgcaa
     6481 taaaggattc acttaactta atagatgact acgcaccaaa gcaaactaaa aagcaaaaag
     6541 atgcacaaga tgaaatcgca gaaaaggcta tacgagcata tggagataga atagcaagag
     6601 gtagagtaaa ctctagtata attagtcaaa agcaatatat acctcaagga atgttgttaa
     6661 tgacaggaga gaatcttata agaggacact caactgttgc aagattagta gcattagaat
     6721 tggacaagga ctcagttaat ttaaagctac tcaaggaaat gcaagatagt gtaagtttgt
     6781 tgggagaaag catgagagga tatatagagt ggttgttaca cggcatgaat aaagatagta
     6841 gtgacattgg aggaattcta atacacaact ttgatttata taaagaagaa ttagagaaaa
     6901 tagagtatac acatacaaaa ggtggtcatg gtagaagtat tgaatcatgt gcatggctgc
     6961 tggtaggtat atcatccatg ttatcatatt ttgaagataa aaaagtaata tctgaagttg
     7021 agtttaatca atatatacaa tttgctaaag atactatggt gaatgtatta attaagaata
     7081 ataatatcac aaaagaaagt tcaccaatag atgagttttt atacacgata aaagaagcaa
     7141 tagattctaa ctctattaag ataagtactc tagtagatgg aaataaatta aatgacaatg
     7201 atgatgatac atatggatat aaggatgata aatattttta ttttcatcca gataagacat
     7261 atagttacgt acaagagata caatcgaaat cgggaaacta tataagttta acaaagagag
     7321 ggttaattaa attattaaga gaacactcga taattaaggt agatagtgat ggatctccat
     7381 aaaagtatac tattaaagta aaagatggaa ctgactataa agagaaaaga tctaggctat
     7441 tacgattagc tataaataca atagagaaat tataagacaa taagagataa atggtgatat
     7501 agaaagaggt aattatatga caattaggat taatttagat ggagaaaata tattagaaaa
     7561 tgtagataat aaattttatg gattaagggc acgtgttgac tttgatcttg atttattaga
     7621 tgaagatgtc atagatatta tggatagaat attaacttta gatgtaaatc catcaagaat
     7681 gtttataaca aaaggtaggg tagatatatt ttatcaacct caaaaggaga tttctataga
     7741 atctaagtta gggtatatga agttaattaa tgaatttata gactttatag aatcgcaaga
     7801 gattgtagaa atgaagtttt taggatggaa cctaactgga gataagattg tagaatcaca
     7861 agcaaatgac agtaatattt catttagcaa agaaatattc aatccagata cagtttatct
     7921 tgatggactt atagagggat atgtgaatct ttagaaaaag ctaagttaga agtatgtagt
     7981 tctaatagga gctgcatact tacttaatac aagattattg agaagttaaa aaaaggaaat
     8041 tattatggag gagaacttat atgaactata ttacaacaca aggatttgat acaataaaaa
     8101 gggataagat agaaataaaa agaatggtgt atgctttaga gaatgagggt gattcgtaca
     8161 atgttattga agaatgtata tgtactttag aagaaaatat agatgtaaat gatgattttg
     8221 tgatagaagt agaaaatgta tttagttttg ttattgatag aataatggac tttataaaag
     8281 agaagaattt gccaaaaaac aaaatagaac atgtaagagc ttatatagat gtatttgaaa
     8341 atccaacttc aaatacaaaa atagtaaagt ctatagatat atatgaagat tatgataaaa
     8401 gatatattga taataagttg gataagttat ttaaaagcat ggataatatg ataaaacccg
     8461 aatttgttcc agtaatagat ggtaaatttg atatacgtgg aagtaaggat ttgaaagagt
     8521 ataataaaaa attaatgtta gacatagtaa agcaaaaaac ttctgaagta aataatggag
     8581 gaataagaat aaataaatta tctgcaatat taaaattagg agataaaagc atagatggca
     8641 taagcaaaat gatgaaggat gtggcaaatc taaagataaa accaaaatca gtatatataa
     8701 gtgaagatac agttgttgta atgtatatat gtaataagag ttgtattgtg cttaatatag
     8761 aagagtattc caagatactg tcagattttg ttgactacat aaataatata gaagataggg
     8821 acttatattt atatgatagc attttatatg gtgactataa aataaattgt ggagtagacg
     8881 atagagatat aattgatttt aattatcaaa gctttaattc aaaagtaact ttgtttgctg
     8941 gacatatagg aaacgagata atacaagctg cattatagaa atatacaatc gtagagagga
     9001 tatagatatg acagaaactt ataaaatcag cataggaaga aaagatttat attctaaaga
     9061 ctataaggcg gttgaaagaa tcgactatga aaagaggtat atagttaata taagtagaag
     9121 cttagtagac atactttcta tacttataga agaagggata taaattatag atatggctac
     9181 atacttagtg tgtagccttt aatatagact taaattagta tatttaaact ttggaggata
     9241 taatatgaaa tcaaagaaag cagtagtata tgcaagattt agttcggaca atcaaaggga
     9301 tgagtcaata gatgctcaat taagggcaat caatgaatat gcagataaga ataatataaa
     9361 aatagttaat cagttcatag atagagcaaa atcagcaaca tcagataaaa gaccagaatt
     9421 tcaaaatatg attaagttct gtgaagcaga tacaacagga atatctatgg taatagttca
     9481 taagctagat agattttcta gagataagta tgattcagct atgtataaac agaaattaaa
     9541 agtaaaagga ataagagttg taagtgtatt agagaaccta gataattcgc ctgaatctct
     9601 aatattagaa tcagttatag aaggtatggc acaatactat agtgctaact tagcaaggga
     9661 agttgctaaa ggtcaaaaag aaaatgctct taaagctcta cacaatggag gagatgcacc
     9721 attaggatat gatgtagcac atgataaaac ttatacagtt aatgaagaag aagcacaagc
     9781 agtaaagatt atatttgata gatatattaa aggatattca tatagtaaga taatagatga
     9841 attaaatgat ttaggatata agactaaaag aggtaataaa tttggaaaga atagtttaca
     9901 tggaatattg aataatgaga agtatacagg agtatatgta tttaataaga ctcagagaaa
     9961 aggtgtagat ggaaaaagaa atggtcatga gcaaaagagt gaagatgaaa taataaaggt
    10021 agaaggtggt ataccagtta taattgataa agaggtattt gaacaagcac aagagatgat
    10081 acaaaaaaga aagaaaactc caggagcaca taaagctact actacatatt tattaacagg
    10141 attaattaaa tgtggagaat gtggacatgc atttcagggg cataagagaa aagactctta
    10201 tggtaatgaa tatgtttcat atagatgtgg atgtagaaag caaaaacgag attatctaga
    10261 agagtttata ttacaagaac ttgaaaagaa catattaaat gatgaggcaa taccagcttt
    10321 aagtaaagca ttgaatgaga agttgaatta taagaatgaa agtaatgcag agttacttaa
    10381 aaacttagat cagaaactag agaaggtaaa taaagagata agtaatatac taaatgcaat
    10441 aatgaatggt atagtaagta gcatgttaaa agataagctt gatgagctag aacaagtaaa
    10501 gttaaactta gatttaaaaa tgaatgaatt aaagatagaa aataaagttg tagatggtgt
    10561 aggcgtaaca gaagaacaaa taagaagtat gttctcaaga tttaagtaat ttgtgctaga
    10621 gaggaacata cctgaatgta agaaatttat aggggactat ataaagaaag taatcgtata
    10681 taaagatcac gttgaagttg tattcaattt ggtcttttct tttgtcgatg atgaaatagc
    10741 tcataactta cacattactg tatcaagagt aaaagtaaaa taaaaacatt aaatataaat
    10801 gttaatttaa atgatattat ggatatattt aacaaataag tagtgttaca tgatataata
    10861 tcacatgtat atgaaaggga taaaatttat actattagta ttggtggtga aatgaatgtc
    10921 aaaaattgaa tactatatga atttacccta tgatttatca ggatcaatgt ctaagaatag
    10981 atttagaaat gaactacttt ggggattgaa aaaaatgtat gagttatata agacgaatga
    11041 agattttgta gtagtttttg attatagatg tgatattgaa gttcatttag aaaataaatt
    11101 tgaattttat caagtgaaaa ctcaaaataa ttcaggtaca tatacgataa ataaacttac
    11161 tgataaaaaa aagggtagta aatcagtagt agggaattta tatagcttga aatatgatat
    11221 aaatatgaat gagattgatg atgtttatgt tgcaattgta tcaaatgctc ctttgaacga
    11281 tggtaaaaaa acatataata attctcatga agttaaaata aaaacaatag atagtagcgc
    11341 tatagaaaag ataaagttaa gtataaaaaa tgacttaaat atagatgaag atataaattt
    11401 agaaaattta ttttttatta gaattggttt agatttaatt gaaccacaaa aaactttaat
    11461 tggtgaaact gctatatttt ttgaagatgt ttttggaagg gaatgtagta aagtaaaatc
    11521 attatacaat gtcttatatg aagagatata tagcaaatca gcatatgagt tagatataaa
    11581 tacgtatgaa aatatagtat ctaagaaagg tattaataga gataggatgg atattatatt
    11641 aaaaagttat gctaataata cagataagtc agtagaacta gcaaaatata gtataaatga
    11701 atggtgcagg aataatttct cacgtagatt atctttgata aaatcattaa ctacaattgt
    11761 atctaaatta agtgaaagta gtattttaaa ggatatagaa aactgtatat tgtcatatat
    11821 atataataat atagataaat tacaagtatc agataaagag ttaattgaaa ttataagtga
    11881 gatagtagaa gaaaagaggc ctatagaagt tagcaagaca gatatagaag ctcttatctt
    11941 actaacatta aaaaagtatg aggagggtgt ttatgaatag tttgtttgtg aacagtctat
    12001 atatatttga atatgaaagt aaaatggcga agaaaataga ctttaaacat gggattaata
    12061 tattaacatc agatgaaaag agtggtaatg acgtaggaaa atcagtacta ttgaaaagta
    12121 tttatcacac tttaggagca gatagtatat ttgatcatat gtggaataaa attacaaaaa
    12181 catatatatt aaatgttact ataaaaaata agacatacta tgtatataga agagataatt
    12241 tatttaaaat atataatacg aattttaaaa aaatatttat gacaaataac cgtgatgaat
    12301 tatctaatta tttaagtgat ttatatagtt ttaaggtaat gctgccaaaa agagaaaatg
    12361 aagaattaga gttagcacct cctgtatata gttatttgct taactatata gatcaggatc
    12421 atttagatgg atctaaattt tcatctttta attcattgac acaatttcct aactataagg
    12481 aaaatgtaat ttatactcat tttggacttt ttgatgaaga gtactataaa gctataaagg
    12541 agttagaaat attaaaatca gaagaaaaaa ataagaaaaa agaacgagaa gttactttga
    12601 atatgctaaa taaaataaaa agttacttag gaaacttaga tgcaccagaa agtatagaag
    12661 ttttaaatat agaattaaat aaaactaaag aagaatattc gaatatagtt ataaacttgc
    12721 aaaaaataaa aaataattta atgaaaataa ggaatgaaaa agtagattta gaattaaaca
    12781 taaaagaaat tgaattaaca agaaggaatg aaatgaagaa gttaaaagtt aaaaatgtgt
    12841 gcccaagctg ttcacatgaa atagaagata tagattctaa atttataata aataatcaaa
    12901 tagaagatct tattattctt aaagatgagt tagagtcaga aattataaag tgcaatagag
    12961 ttatagaaaa gaaagaggaa gaatatcaat cactattaga taaacttcat gagtatgagt
    13021 caaatatgaa aataaatgat gatagcatat ctaatgtctt aaagcataag gcatatatag
    13081 atactaaaga tgatatagtt ttagatttag aaataataga taagaaaata aaagaaatac
    13141 aacagacaat agagccatat gataaaaaaa taaaagaata caacagtctt aagaagaagg
    13201 caaatgctaa gtatgaagag ttaatgaaaa aggctaaaaa agactttgga ttagaagaga
    13261 tagatgaaaa taaaataaaa aatataaaaa ataactttat agcaagaggt agtaatagag
    13321 caataactac tataatgtgg tatctgacat tgttagcagt aaaatatgaa tttaatgaaa
    13381 atgcaataaa gtttccacta attttagata gtccaaataa tgttgaatct gacgagataa
    13441 aggaagaatc attgtttaaa tatatattta aaaatactaa aacagatact caattaatat
    13501 tatcatcgtt agggttcaat gaaatgcaat ttagtgaatt taagtttgat aatataataa
    13561 aacttgaaaa tagcaaatat aatttattaa ataaagagga ttattataat aatatagata
    13621 ttttaaataa aatatttgaa aattagtata ggaatcgtgg gtatgatttc tgtgaatcgt
    13681 ctccaccatt gaaatccacg aaaacacatt gatattttat caatgtgttt tttaagtaat
    13741 agggggaaat atgaaagata gatttaactt aaaaagaaaa agtattcagt cattaatgaa
    13801 taaaaatgta gatgtttatt cttgtaaaga ttgtatagat gattttatag aatcaaattt
    13861 taaaatttat agaagttatt atatatcaga tgatgatata ttagagatta tggatggtac
    13921 aggagaggac tacaaggatg ttatagcaga tatgttacca gacaaagcgc atataaaatc
    13981 tggagaattt ggtgaaataa cagcaacaga aatatataag agcaatatac aaacaacaga
    14041 aatatatata gttgataaat taagtcatac aagaaaagag gatactaaag ttgctgcaca
    14101 taaaacagat attttaatta ttgaaaaaac ctcagaaggt tttataattc attcttctga
    14161 ggttaagact aagataactg attcagactt taatcctatt gaagcaatga tagagggggt
    14221 aaatgatgat atagttacac gtgttgcaga gacactgaat tggattcaaa gaaaactgaa
    14281 aacagaaaag aaatttgatg aattaaaatt tataaaacaa gtaatagaga gtatagaaaa
    14341 tcaaaatgta gaaaatgaat acaatggtgc agtttttata gatgaatcaa aattagacta
    14401 cgagataaat aaaaatatct attgtgatgt taagctttct aaaagccaaa aaggcaaata
    14461 tgacaatgaa ttaaagtcta taggatgtgt catagatggt gataagatta ggtttgatag
    14521 cgtgaacata gatgatatta ataaactaaa tattaatagt cgtaaaagaa attcaataga
    14581 aaaattatat aataaatctc aaagaaaact agaacatata gatgatatta aaattcaaat
    14641 agtaactttt gatgatatta ataaacattg tttagatatg tatgagaaaa ttattcatgt
    14701 agggagaata taatgagtat taataataat ataaataaga ttaggaatct tgatatacaa
    14761 ttagcaaaat atgattgtat atcaagaatg tctgcaaaag aaacatcgaa gcattatatg
    14821 cgattatact ttaaatttat aaaattatta gaactatatg aagatagtag tttaaatata
    14881 aacaatggag aatactcaaa tctagctaat gacttaataa tattttctaa atatgatatg
    14941 tcgagtagat ttaaaggaat tgatgaggaa agaaacttag tatatatagc agttatatat
    15001 tacatattgg gatatagatt tgagtgcaac ttaatattag atatattaga tatgcatgat
    15061 atagaaaatg aatatttaaa aataataatt tatttaatta gaaataaata ttataaatgt
    15121 gatgtattat cttgtataaa aaaatattat atagaagatg agagtaattt aagtttaata
    15181 ataaaaactt tgtatgaaaa agagcttgta tgtgatacta aagatcagac ctatatacaa
    15241 attattattg ctatattaag aaatatacaa aataatggat tctatagagt tatttcaaaa
    15301 aaatcaaaat tagatataaa tatatggaga aaatttgtac aacaaagtat aaagtatgat
    15361 caaatcatat atgatcttcc caaatatcat tatgatttat taaatgatga attattgaat
    15421 ataaagcatg gatatataaa tatgcccact aattactcta agatagaagt aattaagata
    15481 gtaatattta attatttaca tacatatcaa gataaaaaaa tagcatatat acatgtagac
    15541 aaagatgata gggctataaa tgcaattaaa gaatatttta ctgaatattc aacaagaatt
    15601 gaatttattg aaatagattc aattaataat tatagtgaga tagctactga taacaatttt
    15661 ataatcatag atgattttag taatgtttca aacaaatact atggtacacg atttgaaata
    15721 atgatatcat tagcattaaa attagaaaaa aggctattag tattgaataa taattatctt
    15781 aaaatggact ctaatcaact tgaaaatata aatta
//
LOCUS       DAEMHZ010000098        15738 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000098 DAEMHZ010000000
VERSION     DAEMHZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.098"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(218..952)
                     /locus_tag="KPK72_002712"
     CDS             complement(218..952)
                     /locus_tag="KPK72_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2489910.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            1078..1794
                     /locus_tag="KPK72_002713"
     CDS             1078..1794
                     /locus_tag="KPK72_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2489911.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1928..2620
                     /locus_tag="KPK72_002714"
     CDS             1928..2620
                     /locus_tag="KPK72_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489912.1"
                     /translation="MNNTILVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2610..3791
                     /locus_tag="KPK72_002715"
     CDS             2610..3791
                     /locus_tag="KPK72_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2489913.1"
                     /translation="MKNKSLLNHMFRPILDIIISCALTSMLILTSMYIIKSLYFNSGI
                     SVINQLVYQLRDLRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            4073..4528
                     /locus_tag="KPK72_002716"
     CDS             4073..4528
                     /locus_tag="KPK72_002716"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG2489914.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4518..5243
                     /locus_tag="KPK72_002717"
     CDS             4518..5243
                     /locus_tag="KPK72_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG2489915.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5215..7596
                     /locus_tag="KPK72_002718"
     CDS             5215..7596
                     /locus_tag="KPK72_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG2489916.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7801..9030)
                     /locus_tag="KPK72_002719"
     CDS             complement(7801..9030)
                     /locus_tag="KPK72_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2489917.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9631..10545
                     /locus_tag="KPK72_002720"
     CDS             9631..10545
                     /locus_tag="KPK72_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489918.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10569..11204
                     /locus_tag="KPK72_002721"
     CDS             10569..11204
                     /locus_tag="KPK72_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG2489919.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNTGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11580..12296
                     /gene="pyrF"
                     /locus_tag="KPK72_002722"
     CDS             11580..12296
                     /gene="pyrF"
                     /locus_tag="KPK72_002722"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG2489920.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12322..13365
                     /gene="carA"
                     /locus_tag="KPK72_002723"
     CDS             12322..13365
                     /gene="carA"
                     /locus_tag="KPK72_002723"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2489921.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGPGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13418..>15738
                     /gene="carB"
                     /locus_tag="KPK72_002724"
     CDS             13418..>15738
                     /gene="carB"
                     /locus_tag="KPK72_002724"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG2489922.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVNDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         6355 a         1733 c         2821 g         4829 t
ORIGIN      
        1 ttaaaccagt agattgaatg aattaatagg ttaaattaat atataaaata tttaagttat
       61 aattaatgta ttagacaaaa taaatactat taagaactat taaaatatag attaaagata
      121 ataacatata ataaataaaa aagtaagatt gtgtagatta tataaagatt atttacaatt
      181 aatacaatct tgctttttta tttaaaaatt ttaattatta tttaaaattt tttatcttct
      241 ctttatccca tataaacaat ttctcagaat tatttaaagc accaaataca tttcttttaa
      301 aatcaggcat ctctttattt aaattagcaa taagattttt tatgtactct ctcctagtat
      361 gaccatttgc agggcaggga tttgtgataa taggatagtt agaatctttt gaaattttct
      421 tagtcatata ctcttctatg taaaccatag gtcttataac tgttaaatcc tgtctgtcta
      481 ggtatgtctt tggggaaaaa caatttactc taccctcata aaacatagac attaaaaatg
      541 tctcaattgc atcatcttta tgatgaccaa gagctacttt attacatcca agttttttag
      601 cattatcgtt caaagcacct cttcttaaat tagcacacaa tgcacaagga ttcttttctt
      661 gtcttatatc aaatacaatt tcttttatgt cagtttgaaa ttcataaaaa ggaacattta
      721 tttctttaca caatttatct agtggagaat tatcaacacc tccagtattt aatgttatgg
      781 ctatcaattc aaagttttgt ggagaaaatc tttgataact tttaagtata tgaagtagag
      841 ttaaactatc ttttccacca gaaagtccaa ccgcaatttt atcattttct tgtatcatat
      901 caaaatcttg tatagcctta cgagctttac ttagaagttt ttgtatagac attttatcac
      961 catcctttaa aaatattaaa tttaagattt atttaagatt tacttataaa atacttaagt
     1021 cttatactct aaaattaaca ttacagacaa acataacaaa aaggagaaga tttaattatg
     1081 aaaaaattta aattaatagg agccattata ttagtagtat ttataagtgt atttatatct
     1141 aaagatagat tacttaagaa agataaagat ccaatagtag ctgctcaaca tgattcagaa
     1201 caagatgttg aggcaaattc tcaaaataaa aatcactcaa atagtatatc aaatatatta
     1261 ttggtaaaca agacaaatgg aattagcaaa aactatactc ctgaaaatat tactaaggta
     1321 aacatacctt ttgttgaaga agcaacagag gaagaaaaac aaatggcagg agaacctgca
     1381 aaggcagttg aagatttagt taagcaagct aattcagaag gaatacagtt tttagggtct
     1441 tcagcataca gatcttatga tactcaactg gatacttata caagaagggt aaaatctcag
     1501 gggcgagaaa aggcagatgc atatgtagca aagcctggtt acagtgagca ccaaactgga
     1561 ctttgtattg atttaacaaa tccagaaaga tggtttgttg gaagtacaaa agaagctaaa
     1621 tggttggcag aaaatgctca caagtttgga tttataataa gatatcctga aggtaaagaa
     1681 gatataacag gtacagcata tgaaccatgg catattagat atgttggtaa ggatgctgcc
     1741 gaggaaattt attcaaaagg attaacttta gaagaatatt tacaaaataa ataatataaa
     1801 tgtgtaaata atttgtatta tactggaaat ttagaaatta ataaatttat agattacact
     1861 aactaaaata tagtataatt ttatatgaat aaagctatat acaaagatta agttaggatg
     1921 tgtaaaaatg aataatacaa ttcttgttgt ggatgatgaa aaggaaataa gagaccttat
     1981 agagatatat cttttaaata gtggatataa tgtaattaaa gctgaagatg gaaaagaggc
     2041 tctagatatt ttaaaaaagg aaaatattca tttaatgata ttggatataa tgatgcctaa
     2101 aatggatggg atagaagttt gtagaaaagt tagagaaagt ttgaatatac caatacttat
     2161 gttaagtgca aaatcagaag acatggataa aatacaaggg ataatgactg gtgcagatga
     2221 ctatttgaca aaaccattta acccacttga acttactgtg agagtgaagt cgctgattag
     2281 aagagcatat tattttagtg gtgctaataa tgaagataaa gatgtcataa aggttggagt
     2341 agtaattata gataaggaaa aacatagtgt aacagtggct ggaaatgaaa taattttgac
     2401 atcaagagaa tttgatatat tatatttact agcaaataat aaaggcagag tatatagtac
     2461 agaagaaata tttgaaaagg tatggaaaga gaaatattac caatctaata atactgtaat
     2521 ggttcatatg agcagaataa gagataaaat tgaaaaatat acagatggag aaaaaataat
     2581 tcatactgta tggggagttg gatataaaat tgaaaaataa aagtttatta aatcatatgt
     2641 ttcgacccat attagatatt ataataagct gtgctttaac ttcaatgctt attttaacaa
     2701 gtatgtatat aataaaatca ttatatttca atagtgggat atctgtaatt aatcaacttg
     2761 tatatcagtt aagagatctc agaagtctta taggagagag tttattttat ccatttttat
     2821 ttacaatcac atcattagca atttattcca tgatatcgta caagagaaat aagcaaattg
     2881 tagattttat aagaaagact aatagaaaaa atcaaaaatt agaaatcatt aaaagtgctg
     2941 taaatttaat ggatgaaggt aacttagaaa aaagtataga tattgagtat gacttagata
     3001 tattaaagga taaagaaaag gatgatattg atgaactagc acataaaatc aataatatag
     3061 tccatcaact tagaaatata actattgaag agagacaagc tcagcagaca aaaacagatt
     3121 taataacaaa tgtgtcacat gacttaagga cacctttgac atccataatg ggttatttag
     3181 gagtgataga agaaggtaag tataaagatg aagttcagat gatgtattat gttgatattg
     3241 catatgaaaa atcgaaaaat ttaaatgttc ttataaatga tttgtttgaa ttgactaaaa
     3301 tgcaaaataa tacaataaaa ttaaacaaaa tagaaataaa tttagtagag ttattaagtc
     3361 aagtagtatc tcaatttgaa atttatttta aacaagaatt tatggttagt agaattaatt
     3421 ttaatgaaga aaaattaatt gtaaaagctg atccaaataa attggtaaga gcatttgaaa
     3481 atcttataac taatgctgtg aaatatggaa aagatgggca ttatgtagat atagtcacag
     3541 agaaaaagga agatatggcg gttgtaaaag tgataaatta tggggaacca attccagtgt
     3601 tagatttacc aaatatattt gatagatttt atagagttga aaaatcgaga aatagaaatg
     3661 ttggaggttc tggattgggt cttgcgatta caaaaaatat tgtaaattta catgatggag
     3721 aaattactgt ttcaagcgat aaatatcaaa ctgtgtttga agtacaactg ccaataatac
     3781 aatcagctta gttataaaat aaaagtacga ttatgtgata taaatattat aacaataaag
     3841 tgtttacaaa aataaaaaag taatatataa tttaagtcag tttaataaga tacttatcaa
     3901 gagttagggg agagagttag ctcagtgatc ctataccaac cttttatcag gtggtcctgc
     3961 taacaacgat gagatgactt gaaataatat actcttctta tcaataatag ataggaagag
     4021 ttttttttag aattaatgta acaaatttta aagtataatg gaggattaaa taatggaaaa
     4081 agtagaaagt tttaaactag accatacaaa agtaaaagct ccttttgtaa gaaagtgtag
     4141 tgtacttgat ggagtaaagg gagataaagt aactaaattt gacttgagat ttttacagcc
     4201 aaatgtagaa agttttggaa ctgctgctat gcacggttta gagcatcttt tagctactta
     4261 tttaagagat acattagatg gtgtaataga cttatctcca atgggatgta gaacaggatt
     4321 ttatttaata ctatggggag atgtagatgc caaaacagtt aagattggtt tagaagaagc
     4381 attaaaaaaa gttcttgaat cagataaaat gccagcagct acagctatag aatgtggaaa
     4441 ctacagagac ttatctttat ttggggcaaa ggaatatgca aaagatgttt tagataaagg
     4501 attttcttta aatatatatg gagaataatt taattggaag agaagatata agcataaatc
     4561 aaagtgaagt tttaagatat ttacaataca gaggtcaaga gatagataaa gaccttaaat
     4621 atactattga tgaatgtgta gctataacta aaaataaaat aaatcctaga tatttatcta
     4681 ggatttatcc tattaaaata aataaagata atgttgtaga attagaggga acaaatttaa
     4741 cattagagag taaagatgta tatgaacttt taaaagaatg taatgagtgt gtaattatgg
     4801 cagctactat agggatagat atagaaaaag aaataagacg acactcttat tcaaatttaa
     4861 caaaaggact aataattgat tcatgtggaa caacagcaat agaagaagtg tgtgacattg
     4921 ttcaaaataa tttagaaaag gtttttttaa agcaagataa atttataact atgcgatata
     4981 gcccaggata tggagattta ccaatagaaa aaaatataga tatattaaac gttttgaatg
     5041 ctcaaaaaca aataggtcta acaattactt ctaatggaat tatgataccg agaaagtcag
     5101 tagtggctat tataggtatt tctaatacaa gaataaataa atctaagaaa gaaaagtcgt
     5161 gtgaaaattg taaaaactat agaaactgta catatagaaa aggagcgaac tcttgtggaa
     5221 ataagacaat atctgaaaaa taatatatta atatttgatg gtgcaatggg aacaatgctt
     5281 caacaaaaag ggttaaagtt aggtgaaaat ccagaagtat ttggtcttca aaatccagat
     5341 aaacttatag aaatacatac agcatactta gaggcaggtt cgaatgtaat tcttacaaat
     5401 acttttggat gtaatgaact aaaattagat agtaagtata ctgtagaaga agtgattgac
     5461 aatgctgttt tggtggctag aaaagctata gaaaatgtag ataatactaa accaagatat
     5521 gtagctttag atattggtcc tataggagaa atgctagagc ctatgggtac tttaagtttt
     5581 gataaagcat atgaaatatt taaaagacaa gtactacaag gcgttaaatc tggagttgat
     5641 gtaatagtaa tagagactat gatggattta tatgaagcta aagtagctgt attagcagca
     5701 aaagaaaact ctgatttacc tattttctgt actatgacat ttgatgaagg tggaagaagt
     5761 tttactggat gtatgccaga atgtatggtg gctactatag aaggtctagg tgtagatgca
     5821 ataggagtta attgttcatt aggacctaaa caacttttac caatagttga aaaaatagca
     5881 tcaagagcta cagtccctgt tatggttcaa gcaaatgcag gtcttccaaa tatagttgat
     5941 ggagaggcta tatatgatgt cgatgcaaaa gaattttttg aaggggttaa aaagttcgtt
     6001 gaagttggtg ctactataat aggtggatgt tgtggtacga atccaagttt tataaaagaa
     6061 atatctgaga atataaattc tgttacaaag ggatgtatcg agaaaattga taaatgtgta
     6121 gtctgttcac catcaaaatt tgtggaagta caatcgccta cagttgttgg agaaagattg
     6181 aatcccacag gtagaaaatc tctacaagaa gcattaaaaa atgaaaatgt tgattatgct
     6241 ataaatttag gtttagaaca agtaaatgct ggagcacaaa tacttggagt aaatgttggt
     6301 cttccagaga ttgatgaaaa aaaactaatg ccaaaattaa taagagagat tcaagcagta
     6361 gtagatacac ctttacaagt tgattcttcc aatgtagagg ccttagagca aggacttaga
     6421 tattataatg gtagaacaat agtaaactct gtaaatggca aagaggagtc tttagaaagt
     6481 atattaccaa tagttaaaaa gtatggttca tgtgtggttg gacttacact tgatgaaaag
     6541 gggataccta aaaaggctga agaaagattt gaaatagcaa aaaggatagt taatagagca
     6601 gtttcttatg ggataaaacc aaaagatata tttatagact gtttatcatt aactgtttca
     6661 gcacaacaag aagaagcaat agaaactata aaagctataa caatggtaaa gacactagga
     6721 gtaaaaacta tattaggtgt ttcaaatatt tcatttggtc ttccaaatag gaaagcactt
     6781 aatgcttctt ttctaacatt agctttaggg gcagggcttg atttagcaat tataaatcca
     6841 aatgaatata gtatgatgga agctatcaat tcttttaaaa tattaaataa cacagataaa
     6901 ggatgtataa actatataaa tcaatatagc aatataaata attcaaaaaa atcggattca
     6961 tctacaaaga ttgataaaga tttgcccttg gatattttag ttgaaagagg attaaaagat
     7021 gaagcaaaaa atgtaacact gaatctttta aaagagaagg atgaaaatta tatattagat
     7081 gagattttaa ttcctgctct tgataaagta ggaaaaagat atgatagtgg agatatattt
     7141 ttaccacaat tgattcaatc agcagaaacg gtaaaagttt cattaaatac aataaaggaa
     7201 actttattaa gtaaaagtag caataatgta tccaaaggaa aaataatagt tgctacagta
     7261 aaaggtgata tccatgatat tgggaaaaat atagtaaaaa taatgctaga aaattatgga
     7321 tatgaagtta tagatttagg taaagatgtc cctattgaag aagtagtgga agttgctaaa
     7381 aagagaaata taaaattagt aggattaagt gcacttatga ctacgactgt tcaaagtatg
     7441 aaagatacaa ttcaggcact gagagataat gagataaatg caaaggtatt tgtaggaggt
     7501 gcagttttga cagaagaata cgctgaagaa atgggtgctg attattattc aaaggatgca
     7561 aagtctgctg ttgaaatagc aaagttaaat ttttagcata taaattaaaa taaaggtttt
     7621 actaaatagg atattttata tataatataa atataagttt tagatactta tgtttaaaac
     7681 aataggaatt aatctataat aatgagatta attaatataa aaaatattta tcatcaacat
     7741 taaaaagggc tgggaagtct ggtataactt ccatcagccc tttttagtta aaatcatatg
     7801 ttaaaaagcc caattaccat ttttaaaaat ctgtatttgc tttccatcat aagtctcacc
     7861 aataatatcc atatcagcag aaccaatcat aaaatctaca tgtgttaaac tgtcatttac
     7921 accatgttca tccaattctt catcttttaa attatttcca ccttccagac aagtcttata
     7981 agcagaacca attgcaaagt ggcaagaagc attttcatca tatagagtat tataaaaaat
     8041 tatatccgta ttggatattg gtgagttgta tggaacaaga gcgacttctc ctaaataatg
     8101 agagccttca tccgtttcaa taagtttttc tagtgtatct agtccctttt cagcagagaa
     8161 atctattatt ttaccatctt taaatgttaa gaagaaattg tcaattatgt ttccaccata
     8221 aacaagaggt tttgtactat aaacagttcc atttacttta aacttatgtg gcattccaaa
     8281 aatctcttca gttggaatat tcggattaaa acttattcca ttagggtctt tggatgctcc
     8341 acttagccaa acatgaccct ttggcaagtc tagagtcaaa tcagtttttg aggatttata
     8401 ttttaaagtt ttaaagttat ttttatttag aaagtccatt ttagatttaa gattttcatt
     8461 atgttcttcc catgctttta tagggtcctg accatctact cttgaacatt taaatatagc
     8521 atcccacagt ttttctattg ctttatcttc tgataaattt ggaaacactt tttttgccca
     8581 atcttttgtt ggcacagaga ctatactcca tttacattta tcagttagag tgtaagaacg
     8641 ccattctttt aaagcttttc cagatgcctt ctgaaaattt gcaactcttt cagggtcaac
     8701 atcttttaat aaatctggat tttgggcata gactgataaa aatgccccac cttcttttgc
     8761 tatatctaca tattgttctg ctgtccattt ggggaactca tgaaatgctt catctggagc
     8821 atataagtat ttaataagag tactttcttc atcagaccac tctatataaa catttttagc
     8881 accacattta tatgcttctt taagtgcatt tcttgcaaaa tcagcacatt ctattggtga
     8941 ctttataagt agaatttgac ctggctgtat gttaactcca acttttattg ctaattcagc
     9001 gtatttttct aaatttctat ttaaattcat atttatcctc ctatattttt aatattgaat
     9061 aacttataaa gtattataac attacttatg tttttaatat tgaacaactt ataaaatatt
     9121 ataactttac ttagattttg ggtcatattc aaaatattta tactttatag attgagaaat
     9181 aaaaaaaata ttttaattaa aacaaattat gaggataaca aacttattta agcagtatgt
     9241 tttttgttga taataatatt aaagtagaaa taaaaatact tttttagtga aaagctatac
     9301 aagaataccg aaaaaaactt ataatataat aatgagatag atatttataa atgaaaaaaa
     9361 atttttctgg caaagcttaa ttaaaaaggt aatagctagt gttgttattt aaattagcat
     9421 agaaaatcaa ataaaaaaat tatgctaatg tatagacatt gataaggtag aatttaagcc
     9481 ttttgaatgc tatattaaac tagagtagat attcttatga atttcaaaat agggcaggag
     9541 gaaaaaggaa ataaaactat aaaaaatata aagtatttat ataaatttat agagtttata
     9601 cgtcagaaga tttcagttag gagggcataa atggagcttt atgaagccat tttttataga
     9661 aaatctgtaa gaaatttttc cagtaagaag ataaaaaagc cattgatgga agaaataaaa
     9721 agaagttgta aaaatttaga atacttaaat gaagatttaa atattaaagc acatgttgtt
     9781 gatagaggtc atataataca ttttttgatg ggtaaggaat gtaaagtaaa ggcacctcat
     9841 tatatagtag ttacatcaga caaaggtgag gactatttac agaatatagg atttgctaca
     9901 gaaggtgtag ttttgcgcct tacaacattg ggaatagcta cttgctggtt ggaaggaaac
     9961 ttaaagagag aagatatctt ggaatttgta gatattggga ataagaattt tgaagaagat
    10021 gaggaagatg atttagatta tctagctgga ataaaaacag aagaagatga tgaaaacaaa
    10081 atagaaaatc cttatataat aatagcattt ggatatccag caaaaaatga agagttgttt
    10141 aggacgagat atgctagacc tgatagaaag ccagtaaaag atatgataaa taagataaat
    10201 aggaatagaa agtggaaaga tatcttagaa ttgacaagaa gagctccatc tgtaaaaaat
    10261 tctcaaccgt ggtatttcca taaggatgaa cgaggtcttc atttatttga aaagagacct
    10321 aagaaacact gtgaagatat gaataaggtt agcttaggtg tagccttaag acattttgat
    10381 atagcttgta taaaaaataa aatagatgtt tcatatgaaa aacttcctat aaggaataaa
    10441 ataggaaaat catattttat aactgtagtc gagcatgtga aaccagaaga agaaacccaa
    10501 gaagaaaatg taactctaga aaaagaagaa tcgcaagatg aataaaattt tggaagggga
    10561 tattggatat gtataagtgt ggaaaatgta atcttagagg ctgttctaat ggaaatatgg
    10621 ataaattaat gaagatatgt ccaactagag aaattgaact acaacaaaaa gcaaagcaac
    10681 tttatgaaga agaagagaat agaaaaatag cttataatac tggattagtt gaagcagagg
    10741 ggtattgtca atatactaga ctaaaagaga ttatagagtt tgctaaaaag tgtggttata
    10801 aaaagattgg catagcattt tgtattggac taaaaagaga aatgcaaaca gtgcaaaaaa
    10861 tatttgaaca tcatgggttt gaagtatttt ctgttatatg taaaaatggg gcaatgccta
    10921 agagtacaat tggactagaa gcatctcaaa caatatcaaa gtgttcagat gaggtaatgt
    10981 gcaatccaat aggacaagct ttatttttaa atgaagctaa ggttgacctt aatgtaattc
    11041 ttggattatg tgttggtcac gatactttag ccatgaaata tatggaagct cctataacaa
    11101 ctttagttgt caaagataga gtaactgggc ataatccagt tgctgcaata tataatgcag
    11161 aatcatattt tgagaaaaaa ttgtttgata aagatgaaga ataattcaaa gacttattga
    11221 cataaaaagc aattagtggt ttaattataa aaaagataaa actttaaatt atcctataga
    11281 tacataaagt tatataaata aagaaaaata ttacagctaa attccttaaa gtagtccaga
    11341 gagactaaag ggttatagat aatgttagta tataacctct agtaaatctt ctagaggttt
    11401 ttgatgagta taaaataaaa atttaaatat aataaagtct tttaaactag tccagagaga
    11461 ctgggaaaga tatctcatag ctaatgaata gagttatgta tagataaaat ccttatgcct
    11521 cttttggtgt gaggattttt ttatgattat ttaggcttta ttaaaaagga gatgtaaaaa
    11581 tgataaatgg taaagaaaaa ttgatagttg caattgatac tgacgaattt gataaagcta
    11641 aagaactaat agacagatta gaagatagtg tagacatatt taaggtaggg ctagagcaat
    11701 acgttgccac aaaaggtaaa acaatagatt atcttaaaga aaaaggtaaa aaaatatttt
    11761 tagaccttaa attccatgat ataccaaata ctatgaaaag tgcagtgaaa gctgcagtga
    11821 gagataatgt ttggttaatg acaatacatg tatcagatat ggaaggaatg agacaatgtg
    11881 cactaattgc tagggaagaa gcagaaagat taaatataga aaagccatta gtagttggag
    11941 taacagtttt aacttcatta agcaaccaag atttacagga cataggatgt aatatgacaa
    12001 cagaagagtt agctataaag agagctaaac tggcaaaaga atcaggaata gatggggttg
    12061 tttgttcagc gcaagaagta gataaaatag tagaagcatg tggaactgat tttataacag
    12121 tttgcccagg aataagacct aaatcagcag aggttggaga ccaaaaaaga gttgtaactc
    12181 catcagatgc aataaaaaaa ggtgctcatt atttagttgt tggaagacct ataacaaaag
    12241 cagaaaatcc aagagaatca gctattaata ttgtaagaga aatagaaaat gcttagataa
    12301 aatatttggg aggtagctta catgaagggg aaattaattt tagaagatgg aacagtattt
    12361 gaaggaaaag catttggata tctaaaggaa gctattggag aagttgtatt taatacttca
    12421 atggctgggt atggagaact attgacagat gcaacttatt atggacaaat agttactatg
    12481 acttatccac ttataggaaa ctatggaata aatttagaag atgttgaatc aaataaggtg
    12541 caagtaaaag gacttattgt aagagaaaaa agtgataacc caaataattt tagatgtgaa
    12601 atggatatag acacatatct aaagcaaaat aaggtaatag gacttgaagg aatagataca
    12661 agagcattaa ctaaaattat tagaaacaat ggaactaaga gaggaattat aactttagaa
    12721 aatgtatcat tacaagatgt tcagtctaaa ttagaagagc attgtaacaa agattcagta
    12781 aaaaatgtta caagaaaaga aattgaaaat ataaaaggtc ctggagctaa agttgtagtt
    12841 atagactttg gtgttaaaaa tagtgtacta gagtctctta aggcttgtaa ctgtgatata
    12901 actatagtac cagcaatgac tacagtagaa gaaattttaa atataaatcc tgacttaatc
    12961 ttattatcta atggcccagg aaatccagag gatataaatg atatagttga aaatataaaa
    13021 aaattaatag gcaaaaagcc attgacagga ataggtttag gacatcaaat tttagcattg
    13081 gcattaggag gaaagacttc aaagcttgta tttggacata gaggaggtaa tcacccagtt
    13141 aaatgtttag aaactggaaa ggtatttata acttctcaaa atcatggata taatgtttca
    13201 gaaatgccag aaaatacaga agtaactcac ataaatttaa atgacaatac tatagaagga
    13261 atgagacata aggaattacc aatatatagt gttcagtaca ttccagaata cacaacaaaa
    13321 gaagatttag attgtatatt taataaattc ttagcattaa tatagattta gtattattta
    13381 cagatagtaa aaaccattta gataaggaga aaaatttatg cctaaattag atagtataaa
    13441 gaaaacttta gtattagggt caggaccaat aataataggt caagctgcag agtttgacta
    13501 ttcaggaaca caagcttgcc agtctttgaa agaggaaggg attgaagttg tattaataaa
    13561 tagtaatcca gccactataa tgactgataa agaagtggca gataaaatat atatagaacc
    13621 attaacaata gaatttatag aaaaaataat agaaaaagaa agaccagaca gtttacttgc
    13681 aggtatgggt ggacaaacag ggctaaacct agcagttgaa ctttatgaaa aaggcatact
    13741 agataaatat ggagtaaaaa taataggtac ttcagtagaa tctatcaaaa aaggtgaaga
    13801 tagagatata tttagagaag taatgaaaga aataaatcag cctgtaatag ttagtgatat
    13861 agttacagat ttacaagcag gtcttgatta tgcacttaca attggttatc cagtagttgt
    13921 aagaccagca tacacactag gtggaactgg tgggggtata gctgataatg aggaagaatt
    13981 aagagaaata ctttcacatg gtctacaatt aagtcctgtt ggacaagtat taattgaaaa
    14041 gagtataaaa ggttggaaag aaatagaata tgaggtaata agagattcaa agggaaattg
    14101 tatagttgta tgtaatatgg aaaatataga cccagtcgga gttcatacag gagacagtat
    14161 agttgttgct ccaactcaaa ctttaagtaa caaagaatgt gcaatgctta aaaaagcatc
    14221 tttagatata ttaaatgcag tagaagttca aggtggttgt aacgttcaat ttgcacttaa
    14281 tccacatagt tttgaatatg cagttataga gataaatcca agagttagta ggtcatcagc
    14341 tttagcatca aaagctacag gatatccaat agctaaagtt tcgtcaaaaa tagctttagg
    14401 ttatacttta gatgagatag aaaatgcagt aactaaaaaa acttatgctt gttttgaacc
    14461 aactatagat tatgtggtgg caaaaatacc aaaatggcct tttgataaat ttaaaaaagc
    14521 aaatagaaaa ttaggaacta agatgatggc aactggtgaa attatgagta taggaagtaa
    14581 ctttgaggct gctattctta aagggataag atcacttgaa acaggaaaat attcattagt
    14641 tcacacacca tctgaggaga gaagcattga ggaattaaag aaaagagttg tagtacctga
    14701 tgatgagaga ctatttgatt tagctgaaat gataagaaga ggctataaag tagaaatgat
    14761 agaacaaatt acaggtatag ataaatggtt tataaataaa tttaaatgga tagtagagca
    14821 agaagaaaaa ttaaaagtta tgaaaattga agatttaact aaagactatc ttcttgaatt
    14881 aaagaaaaag ggattctctg ataaaggtat atctgaccta atgaagataa gtccagaaaa
    14941 attatatgaa ttgagaagtc tatacaatat caaaccagca tataaaatgg ttgatacttg
    15001 tgcaggtgag atagatgcta tatctcctta ctattattca acttatgagc aatatgatga
    15061 ggtagttgtt aatgataaga aaaaggtgat agtattaggt tctggtccaa taagaatagg
    15121 tcaaggtata gaatttgact attgttcagt tcactgtgta aaatctttaa gaaaaatgga
    15181 catagaaact ataatcataa acaataaccc tgaaactgta agtacagact ttgatacatc
    15241 agataagctg tattttgaac cattaacaga agaagaagtt ttaagtataa tagaaaaaga
    15301 aaagccagaa ggtgtaatac tacagtttgg tggacaaaca gctataaaat tggctaaatt
    15361 tttacatgaa aaaaatatac ctatattggg aactgatttt gatgatatag atgcagcaga
    15421 agatagagaa aaatttgatg atttactaga gagactagat ataaatagac caaaaggaaa
    15481 gggtgtatgg tcacttaacg aaggtattga agttgctaat gagttaggtt atccagtatt
    15541 agttagacca tcttatgtac ttggtggtca aggtatggaa ataacttata acgaagaaaa
    15601 attatctcaa tatctacaag atgcatttga tagagaccat aaaaatccag tacttataga
    15661 taaatatcta actggtagag aaatagaagt agatgcgata tgtgataagg aagatatatt
    15721 gatacctggt ataatgga
//
LOCUS       DAEMHZ010000099        15489 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000099 DAEMHZ010000000
VERSION     DAEMHZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.099"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            275..925
                     /locus_tag="KPK72_002725"
     CDS             275..925
                     /locus_tag="KPK72_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2489923.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVEHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            1099..2031
                     /locus_tag="KPK72_002726"
     CDS             1099..2031
                     /locus_tag="KPK72_002726"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2489924.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKYNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFDNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            2305..3018
                     /gene="ispD"
                     /locus_tag="KPK72_002727"
     CDS             2305..3018
                     /gene="ispD"
                     /locus_tag="KPK72_002727"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG2489925.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            3015..3500
                     /locus_tag="KPK72_002728"
     CDS             3015..3500
                     /locus_tag="KPK72_002728"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG2489926.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    3595..3803
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3867..5582
                     /locus_tag="KPK72_002729"
     CDS             3867..5582
                     /locus_tag="KPK72_002729"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2489927.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLVSQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            5704..7149
                     /locus_tag="KPK72_002730"
     CDS             5704..7149
                     /locus_tag="KPK72_002730"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2489928.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            7589..9070
                     /locus_tag="KPK72_002731"
     CDS             7589..9070
                     /locus_tag="KPK72_002731"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG2489929.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            9291..9446
                     /locus_tag="KPK72_002732"
     CDS             9291..9446
                     /locus_tag="KPK72_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489930.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            9591..10988
                     /locus_tag="KPK72_002733"
     CDS             9591..10988
                     /locus_tag="KPK72_002733"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG2489931.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            10973..11386
                     /locus_tag="KPK72_002734"
     CDS             10973..11386
                     /locus_tag="KPK72_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG2489932.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            11492..12250
                     /locus_tag="KPK72_002735"
     CDS             11492..12250
                     /locus_tag="KPK72_002735"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG2489933.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            12263..13000
                     /gene="rlmB"
                     /locus_tag="KPK72_002736"
     CDS             12263..13000
                     /gene="rlmB"
                     /locus_tag="KPK72_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG2489934.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            13003..13530
                     /locus_tag="KPK72_002737"
     CDS             13003..13530
                     /locus_tag="KPK72_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG2489935.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            13595..14242
                     /gene="sigH"
                     /locus_tag="KPK72_002738"
     CDS             13595..14242
                     /gene="sigH"
                     /locus_tag="KPK72_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG2489936.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            14316..>15489
                     /gene="tuf"
                     /locus_tag="KPK72_002739"
     CDS             14316..>15489
                     /gene="tuf"
                     /locus_tag="KPK72_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2489937.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT         6449 a         1615 c         2982 g         4443 t
ORIGIN      
        1 taatagtata gcattatata gctatttcat tgagttggaa gagtgcttat atccagattc
       61 actaaaattt agaaataaaa aatttgagaa taagaacata taaaaataaa aaattgaatt
      121 gaagcctagc ttaatctttt ttgataaaat ataagtttga actttatact aaatttttag
      181 aattttcaat caagacaata acataatagc taagcaatta gaacacaatt aagatgtata
      241 aggcatgtat atgatgaatg ggaggattta attaatgtta ttagagaaat taagaaaaga
      301 agtattacaa gcatctttag accttttaaa ctataattta gtgacattaa caggaggaaa
      361 tgtaagtggg agagatgaac aaactggcta tatagcaatc accccaagtg gaatggatta
      421 tagaaatctt acaccaagtg atattgttat agtagatgta gatggaaata ttatagatgg
      481 aaaatggaaa gcatcagtgg atttatcaga ccacttgtat atatataagc atagagaaga
      541 cataaatagt ataattcata cacattctac atactcaagt tgttttgcta ttttaaatga
      601 acctatagaa tgcgcaagta caactcttgc aaatgaagta gggggttcag taccagttgc
      661 aaaattttca ccaccaacat ctaaaaagat gggaaaatgt gttattgaag caattggtga
      721 caaaagagca tgtcttttag caaatcatgg cgtaattgca gttggtccat ctgtagaaca
      781 tgctcttaca gccgctgtaa tgttagaaga ttcagcaaaa gtatattact tagcaaaatc
      841 aattggcaca ccagtgctac tgcctgatga agaaattcaa agagcgagag atgtattttt
      901 taatgtctat ggtcaggata aataaattag ataaaatttt aaaagtaata tgaaaatata
      961 gatattcaat gatattaagg agatttataa tgatataata agtttaatag ttaagaagta
     1021 aaatatgaaa tgtaatagta tataaattca tttaagacta aaaataataa gataaaataa
     1081 aaaggagtct gtatttctat gtattactta ttaaacaaaa gcacaaatcc ttactttaat
     1141 ttggcattag aagaatatct atttttaaat gataaatata atgatgatat tataatcata
     1201 tggagaaatg aagaatccat ttttataggt aaaaatcaaa atccatatca ggaagtatat
     1261 catgatgtta ttgaaaaggg agaaattcct attttaagaa gaatatctgg aggaggaact
     1321 gtttatcatg acttaggtaa tatcaatatg tcattcatac aaaaagatag gcaacttcat
     1381 gagatagatt ttttggagca tactaagttt atgcaaaaga tgctttcaac cttaggacta
     1441 gatgtatcta taactgaaag aaaagattta tttttaaatg ggaaaaaaat ttcaggttct
     1501 gcacagtcta taaaaagaaa aaattctctt tatcatggaa ctcttcttta cgattcagat
     1561 ttaaataaat taactaaata tttaaattca aataaagcaa ctgaatcaaa tgcaactaaa
     1621 tctgtttcaa gtaaagttac aaatattaag tttttactcg aaaaagatat aaattacttt
     1681 ttagattatt gtgttgaata tttaaaatat aatataaaga atataaaaga acttgaattg
     1741 gatgaagaag atattaaaag tgtatacaat ataggagaag ataaatataa aaagatagag
     1801 tggacatatg gtaaaacgcc gaaatttcaa gtagtgctac catttgataa tgaaagtaaa
     1861 ataaatatac atgttaacag atggaaagta tcaaaatttt ttatttctta tgatgataat
     1921 gtaattaata tggacaagtt gttagatttg aatttcttta aagaagaaat aaaagagagt
     1981 attttagaaa attatccaca ttacatagat ttaatcaatt taatatttta atataaaacc
     2041 tctaatttga taaaattgtc ttatatcaaa ctagaggttt ttaatattct tgcatattaa
     2101 caaccattac aaaattgtaa aattaaaaac aacactattg aaaaatcaat atatatttgt
     2161 ttaaatatat gtgtttttca ttagcaaata gacattaact atttatacaa ctgaaaattt
     2221 aatatataat taatcagatg gttaaaataa aaatattaaa agttttaata aatgatggtt
     2281 aaaaataaaa tacgaggtga taccatgtat agtgttataa ttgttgcagc gggaagcgga
     2341 aggagaatga acttagatat aaacaaacag ttcataaaat taagagaaaa agaaattatt
     2401 gctcatacaa ttcaagtttt ttatgaaaat ataaatatag atgaaatagt ggtatgtata
     2461 aaaaaagaag aagaggattt ttttaaagaa aatataataa ataagtataa ctttaaaaat
     2521 ataaaaatag catatggtgg aaaagaaaga caggactcaa tatataatgg tttaaaaaaa
     2581 ttagataaaa attgtgatat agtacttatc catgatggag caagaccatt tgtcgaccat
     2641 agaataatca atgaatctat aaaggtagct aaagaaaaaa aggctgtggt tgtaggtgtt
     2701 ccagtaagtg atactataaa aatagtgtca gatggaacag tacaagaaac tccagaaaga
     2761 aatttgctgt gggcagcaca aacccctcaa acctttgaat ataatctcat aatagatgct
     2821 tatgaacaag catataagaa caattattat ggtactgatg attcaatgtt ggtagaaaat
     2881 ataggtcaaa gtgtcactat ggtaatgggt tcttatgaaa acataaaaat aactagtcca
     2941 gaagatttaa atatagcaga acaaatatta aatatggaaa aaagagatga agttaacagt
     3001 agaaggagaa ttatatgaga ataggattag gatatgatgt acacaaatta acagaggata
     3061 gaaaacttat aatcggtggt gtagagatac cacacgataa aggtttgttg ggtcattctg
     3121 atgctgatgt attgatacat gcaataatgg attctatttt aggtgctcta gcattaggag
     3181 atatagggaa gcatttccca gatacagatg aagaatataa gggagcagat agtatgaaat
     3241 tattagaaca tgtgtataat ctaataacaa gtaaaggata taaaattggt aatatagaca
     3301 gtactataat tgctcaaagc cctaagatgg ctccatatat tgagagtatg agaagtaata
     3361 tatcaaaagt tttaaacaca gacattgata atataaacat aaaggcaaca acagaagaag
     3421 gtcttggttt tacaggagca aaacagggaa tagcttcaca aagtatttgt ttattattgt
     3481 tgacaagtca gaataattaa tatattatta gtaataagtt taatggatat caagtaatag
     3541 gcctatatag aatatctata atgtatgaat ttaagtgaaa ttaatataaa tataaaatta
     3601 gctatgataa agaaatagta aaatgatgaa atcttcacag agagaaaacg gtggtgagag
     3661 ttttcaagag gaagtctttg aacctgtctt ttagcttaaa ggtataaaaa tctttacggt
     3721 aggaaccgtt atattcccaa aagagagcta ttcatatagc taactagagt ggtaccgcgg
     3781 aaattaaacc tttcgtctct atacttgtat agagatgaga ggttttttat attttcagga
     3841 agttaatatt caggaggaat ctattaatga aaatgtcaaa aatgtttatg ccgacattaa
     3901 aagaaatccc agcagatgct gagattacaa gccatcaatt aatggttagg tcaggcatga
     3961 taaaaaaaat gacttcaggt gtttataacc aattaccaat ggggttaaga gtgttcaaaa
     4021 aaattgaaca aataattaga gaagaattaa ataaaaaaga ttgtcaagaa atactttgtg
     4081 ccgccttact tccttctgaa ttatggaaag agtctggaag gtggactgct atgggtgaag
     4141 aaatgtttag actaaaagac agaacagaaa gagagtactg tcttgggcca acacatgaag
     4201 aagcattcac tgatataata agacaagaaa ttacttcata taaacaattg ccactgaact
     4261 tataccaaat acaagtaaaa tatagagacg agagaagacc aagatttggc gttatgagaa
     4321 ctaagacttt tacaatgaag gatgcctata gttttgatgt agatgataaa ggtttagata
     4381 agtcatatca agatatgttt gatgcatatg taagtatatt tgatagatgt ggattggaga
     4441 atagccctgt acaggcagat tcaggagcta taggaggttc tacatcagcc gaatttatgg
     4501 ttaagtcaga agttggtgaa gatgaagttg tattttgtag tggatgtgat tatgcagcca
     4561 atgtagaaag agcagaatct tgtaatctag tatcacaaaa agaagaaatg aaagaattag
     4621 aagaggttca tacaccagga gcagctacaa taaaagagtt agaagaattt ttaaaaactt
     4681 ctccagataa gtttgctaaa actttagtat atgaggcaga tggaaaaaca gttgtagtcg
     4741 ttgtaagagg agatagagaa gttaatgaaa tcaaagtatc taatgctata ggttctgtta
     4801 tagagtttgc tcttgctaca gatgatgtag tgagaaaagt aactaatgca gaagtaggat
     4861 ttgcaggtcc aataggtata aatgctgact atgtatttat agataaagaa attgttgaac
     4921 aaagaaacat agttgttggg gctaataaaa ctgagtacca tataaaaaat gctaactatg
     4981 gaagagattt tgaaggaata gttggagact ttagaaatgt tcaagaaggc gacaagtgta
     5041 tagtatgtgg taagccactt gaaatagcta gaggtgttga agtaggacat atatttaaac
     5101 taggaactaa gtactcagaa tctatgaatg caaacttcat agacaaagat ggaaaatcaa
     5161 agcctatagt aatgggatgt tatggaatag gtgttgaaag aacagcagca gctataattg
     5221 aacaacataa tgatgagaaa ggtataatat ggcctttgtc agtagctcca taccatgttg
     5281 tgatagttcc agcaaatatg aaaaatgaag aacaaatatc aatagctgaa aatatatata
     5341 atgatttgca agctatgggt gtagaagttt tattagatga cagagatgaa agaataggtg
     5401 tcaaattcaa tgattcagaa ttaataggaa taccgatgag gattacagta ggtaaaaata
     5461 ttaatgaagg taaagtagaa tttaaactta gacataaaga agataaagag ataattgaca
     5521 tagaagaaat aaatgaaaaa gtaaaggcag agtttatcag gaataatgtt aggttaggat
     5581 aataacttaa ataagatatt gtatagataa tcaaagttgt actttataga caccatttta
     5641 tctatatata aggatataaa atggtgtttt tttataaaac aaataaatga ggaggtaatt
     5701 gaaatggcaa aaaatgaaaa acaatttgtt gaagaaataa caaagatgga agatgatttt
     5761 ccacaatggt acacagatgt aataactaag actgacttag ttgattatgc accagtaaaa
     5821 ggttttatgg ttgtaaagcc atatggatat gcattatggg aaaagatgca agaatttatg
     5881 gataaaaaat ttaaagaaac tggacataaa aactgttatt ttccattatt aataccagag
     5941 agtttattaa acaaagaagc agaacacgtt gaaggttttg ctccagaagt tgcatgggta
     6001 acacatggag gaaataaaaa attagaagaa agattatgtg taagacctac ttcagagact
     6061 ataatatgta caatgtatgc gaaatggtta aaatcatata gagaattacc atatctttat
     6121 aatcaatggt gctcagttgt tagatgggaa aaatcaacta gacctttctt aagaacatct
     6181 gagtttttat ggcaagaagg tcatacgtta catgaaacag cagaagaagc acaagaagaa
     6241 acaatacaac aattagaagt atataaagcg ctatgtgaag aattacttgc aatgccagtt
     6301 gtagctggtc aaaaaagtga aagtgagaaa tttgcaggtg gtgaaagaac atatactata
     6361 gaagctatga tgcatgatgg taaagcatta caatcaggta caagtcactt cctaggtcaa
     6421 cactttacta aggcttttga tattactttt gcagataggg aaggaaattt agctaatcca
     6481 taccatacat cttggggagc atctacaaga cttataggtg gacttataat gactcatagt
     6541 gataacagag gtttagtatt accaccaaga gtagctccaa ttcaagttgt tatagttcct
     6601 atagctgcta aaaaaggaaa tgtaatggag actgttgata aaatatatgc tgatttaaaa
     6661 gctaagggag tagctgtaga agttgatgat agagataact atacaactgg atggaaattt
     6721 aatgagtggg aaatgaaagg tgttccagta agagttgaaa ttggaccaaa agatatagaa
     6781 aataatcaag ctatggtatt tagaagagat actttagaaa aggattctat gcctttagaa
     6841 ggattagctg atgctatatg tgatttattt gatgttatac acaatgatat gtttgaaaaa
     6901 gcaagaaaac atagagaaga caatacatct atagttgaaa atatggatga gtttagaaaa
     6961 gctttagaag aaaagccagg attcataaag actatgtggt gtggagatgc agaatgtgaa
     7021 gctaagatta aggaagaaac aggagcaact ataagatgct taccttttga acaagaaaac
     7081 ttaggtcaca agtgtgtgta ctgtggaaaa gaagcagata gtatggtagt aatggcaaaa
     7141 gcttactaaa atacatttaa ataaaaactg gggtagaaag tatttaatac tttatcccag
     7201 tttttttgtt ttttatgagt aataaaatgt atttgtaact atgtcataag ataagtcata
     7261 gatacttata ttaaaaagaa tataatagag aaccattttt aataattaca tattaatgaa
     7321 agcaaaatat ttttgttata tagattttca ataaagcaaa caataaatat aaatgaatat
     7381 tagtaaggag tgcttttaat ttttagaaaa taaatgactt aaaagaaaga tttatgaaat
     7441 taaaactagg attttctgat ataataaaca tgtgtatttt taaataaaaa ggatattatt
     7501 attagatttg attaaaaata aacttgcaaa acaatgtact agtttaatat tatagttata
     7561 ttgaataaaa tatcaggagg aagtaaaaat gaatgtaaga gtaaggtttg caccaagtcc
     7621 aacaggattt gtacatatag gtagtttgag aactgcttta tataactatt tatttgcaaa
     7681 aaaaatgggc ggagaatata tattaagagt ggaagataca gaccaaagta gattagtaga
     7741 aggtgctata gagaatatgc ttaatgctat gaaatgggct ggtgtcaacc atagtgaagg
     7801 tgttatatta gatgatagtg gaaaagtagt acaaaaagga gaatatggtc catatattca
     7861 atctcaaaga ttagatatat accaagaata tataaagcaa cttttagata gtggaaaagc
     7921 ttattactgt ttctgtacaa aagaaagact ggatgaagta agagatgctc aaagagcagc
     7981 aggagaaaca gctaaatatg atggtcattg taagaatctt tctaaagaag aagtagaagc
     8041 gaatataaaa gcaggaatac catatgttat aagattaaga ttgccagaga atcatacaat
     8101 taagtttaca gacttagtta gaggagatat ggaatttaat actaatgact tagatgacca
     8161 agtattaatg aaaacagatg gatttccaac ttatcatttt gcagtagttg ttgatgacta
     8221 tttgatgaaa attacacatg ttataagagg agaagaatgg gtatcatcta ctccaaaaca
     8281 tgtttattta tatgaagctt ttggatggga agcacctgta tttgtgcatt taccaaatat
     8341 acttaataaa gaaaagaaaa aattaagtaa aagacaaggt gatgtagctg ttgaagactt
     8401 taagaaaaaa ggatatttac cagagggatt agtaaactat gtagcattag ttggttggtc
     8461 tcctgaagat aatcaagaat tgtttacaat ggaggaatta gaaaaagcct tctctgtaga
     8521 aagagtatct aaaagtggtg gagtatttga tactgaaaaa ttaaattggg ttaatcagca
     8581 ctatataaaa gatggtgatg atgcttatct aacagactta gcaataccat ttttaataga
     8641 agatggattt ataacagaag aagaagctac taataaatat gatttcttaa agagtatgat
     8701 atcagtactt aaagagaaat tacaatatgt aaaagaagtt actgaacatg caagtatatt
     8761 ttttggagat aaaatagagg tagaaactga agagggaaga gagttcttaa gattagaaca
     8821 tatacctact ttaatagatg ctttgagaga aaaaatagaa aaaacagagg tattaaatgc
     8881 tgagtttgtt caagctatgc ttaaagaaat acaaaaagag tacaaaataa aagggaaaaa
     8941 tttatttatg ggctcaagaa taattttgac agggcaaatg catggtccag atttaccaaa
     9001 agtaatggaa gttttaggaa aagaaacttg cttaaataga atagcttatg taaaaaataa
     9061 tatattatag tcataaaagt ccgctaattt agaagcggac tttttttgtg ttagtatttg
     9121 gtgaaaatat ctgcttaaga aaatgaaggt atttaaggta ataaatttaa tatataaaaa
     9181 gcaaagcaaa aatataaata aaatatgaat aataaactaa taaaatatta agcatagaag
     9241 aagggaatct attattttag tttgatttaa ttattaaggg ggtaattact atgtatagta
     9301 atattgatga tgttaaaaag gaattaaagg agctatgctt agaatatgtt acaattttag
     9361 aaaaattaaa agatgaaaaa atgattacag aagaaacttt tgaaaagtgc tcttctcaaa
     9421 aaaaaatatt tttagaagaa caatagagga tggtaatagt attattatta tgtagtttga
     9481 aagatagtac gaaaaatgat agtaatacta ttatttattg caattttcaa ctataatata
     9541 atatataatg ttaagaagat aatttataaa aatacagtga ggtgatgagt gtgaaagttt
     9601 ataatacatt gactagaaca aaagaagaat ttgttccatt agaagaagga aaggtaaaaa
     9661 tgtatgtgtg tggtccaaca gtttataact atatacacat aggtaatgca agaccattta
     9721 taatatttga tactctaaga aggtatttag agtatagagg atatgatgta acttatgtac
     9781 aaaactttac agatgttgat gataagatta taaatagaag tcatgaggag ggaatatctc
     9841 ctgaagaagt tgcggctaag tatataaaag aatattttgt agactgtgat gggctaggaa
     9901 taaaaagagc tactgttcac cctcaggtta cagataatat acaacagatt atagagttta
     9961 taaaagagtt ggaagataag ggatatgcat atgcagtaaa tggtgatgta tattttgata
    10021 caaataagtt tgaaggttat ggaaaacttt caggtcaaaa acaagaagat ttagaggcag
    10081 gggctagaat tgaggttaat gaccaaaaaa gacatcctat ggactttgtt ctttggaagg
    10141 caaaaaaaga aggtgagcca ggatgggata gcccttgggg agaaggtaga ccaggatggc
    10201 atatagaatg ttctgttatg tctaagagat atttaggtga aacaattgat atacatgcag
    10261 gtgggcaaga tttaacattc ccacatcatg aaaatgaaat agctcaaagt gaagctagaa
    10321 gtggaaaaac attctcaaaa tactggatgc ataatggata tataaatata aatgatgaaa
    10381 agatgagtaa atcaaaaggt aatttcttta cagtaagaga tatatcaaaa ttatatgatt
    10441 tagagatagt aagatttttc atgttatcag cacattatag aaatccagta aactttagtg
    10501 atgagatgtt aaatcaagct aaagcaggtc ttgaaagact atataatact aaagaaaaat
    10561 tagaatttac gctaagtaat ttagtagaat ctcctttaac agaaaaagaa gtagagcttg
    10621 ttaaagaatt agatgacttt agacaaaaat tcatagatgc tatggatgat gatgttaata
    10681 cagcagacgc agtaagtgta atatttgaat tagctaagtt aataaattct aatgtagatg
    10741 aaaattcttc tttggagttt gctaaaaaat gcttagatga atttaatgaa cttacaggag
    10801 tattaaatat tgtaaataag aaaaaggata ctgtattaga taaagacatt gaagaattga
    10861 tacaaaaaag aactgatgca aagaaaaata aagagtttca attagctgat gatattagac
    10921 aacaattact agatatggga attgtattag aagacacaag acaaggtgtt aagtggaaaa
    10981 ggatataaaa atggaaaaaa ctgaattagt tactatgtca ccattagttt tagcatactt
    11041 aggtgataca gtgtatgaga catatataag agaatattta attagacaga atacacaaag
    11101 aaaagttaat gatttgcata aactagctat aaagtatgta aaagctaaag ctcaggcaac
    11161 tataattcat gaaattgaaa ttgagttaac tgaagaagag agtaaaattt ataaaagggg
    11221 aagaaatcaa aagtcaaata cttctcctaa gaatgcagat attatagact acaaacatgc
    11281 aactgggttt gaggcattag tgggatattt gtatttaaat aatgaaatag agagacttca
    11341 atatattatc aataaaggaa tcaaaattat tgaaagagat atgtaaatat taaagagtgt
    11401 tgtcttaata ataagtaata aggcaacact ttttttataa ctaatgtata ataaatatat
    11461 agaaattagt aatgagaaag gataaaaagt tatgaaagta aaattaatat cacatacacc
    11521 tgaaccagaa aaagtaatag ctatggcagc taagctatgt tattcaccag ttgggacaga
    11581 cgaaatagaa aaagacttaa cagatgaaag tatagagaaa tttttaaata tgttattaag
    11641 tataggtcat gggtcaatat tagaacatgc ttcatttaca ttttcaatag aaggtatttc
    11701 aagggcttgt tcacatcaaa ttgttagaca tcgtatagct agtttttcac aacaaagtca
    11761 aagatatgtt aaattagaac aatttgagta cataattcct ccagaaatag aaaaaataga
    11821 aaaggcaaaa gagttattta tagactccat gaaaaaagac caagagaatt atgataagtt
    11881 agttgaaata ttgtttgaaa atcattataa tgacctaata aaaaacggta aaaatgagaa
    11941 aacagctaaa agacaagctg aaaagaaagc aatagaagat gctagatacg tatttccaaa
    12001 tgcatgtgaa acaaaaatgg tatttactat aaatgctaga agtttgttta atttctttga
    12061 acatagatgc tgtgaaagag cacagtggga aataagaaat ttagctgtgg agatgttaaa
    12121 agaggttaaa aaggttgctc ctatattatt taaaaagact ggtccaagtt gtgtaaatgg
    12181 aagttgccca gaaggaacta tgacttgtgg agatatagtt caagttagag aaaaatttaa
    12241 ggccttgtag gaggtgaata atttggcgag tatagaagga agaaatccgg taatagaagc
    12301 aataaaaagt gacagagaaa tagataaaat attaattgca aattcagcta aagaaggttc
    12361 aattaagaaa ataataggaa tggctaaaga taagaatata attattcagt atgttgataa
    12421 acataagcta gatgaagtaa gtacaagtca ttcacatcaa ggtgtaatag cttatgcaag
    12481 tgagtataag tattatgaat tagatgagct aatagattta gcaaagaata aagatgaaga
    12541 cccatttttc attatattag atgagataac agacccacat aatttgggtt caataataag
    12601 gacagcagat gcagtaggag cacatggagt tatcattcca aaaagaaggt ctgtacacat
    12661 aactcctgta gttgccaaag catctgctgg agcagtagaa tatatgccag tttgcaaggt
    12721 aaccaatata gttaatacaa taaaaagact caaagaagaa gggctgtgga ttgctgcagc
    12781 tgatatggat ggagaaactt tttataaaca aaatcttact ggtcctcttg gtgtagtaat
    12841 tggaagtgaa ggttttggaa tatctagatt ggttaaacaa aactgtgact ttatagtaaa
    12901 aatgccaatg ataggtaatg ttacatcttt aaatgcatct gtagcaggtg gaatactttt
    12961 atatgagata ttcagacaaa gactggataa aagtaaatag gcatgaaaaa aaatataaat
    13021 cattacttaa taatagatgg ctacaatatt ataaatgcgt gggacaattt gaaagaactg
    13081 gctaaagagg acttagaaga ttctcgagaa aagcttattg atgacattat agaattttct
    13141 gaatttatgg gctataagac tataatagtt tttgatgctt ataatgttaa aaattctcga
    13201 gaaaaagttg aaaaaagaaa acacataaca atagtttata caagagaaca ccaaacagca
    13261 gatagttata tagagaaatt tataacttct ttatcaaagt atgatgatgt aaaagtagcc
    13321 actaatgact atgcagaaca gcaaatgatt ttaggaaaag gtgctactag aatgtctgca
    13381 agagaattaa agttagagtt agatcgttcc aagaataaaa tgaaggaaaa aaatatcggt
    13441 ttgaggaaaa aaattcagcg aaattggttg gaggaaaggt tagataaaga aacattgtcg
    13501 aaacttgaga acattcgtag aaagcgttga aattactagg ttctttagac tataatatac
    13561 ctataaattt gttttatata tgggggagat ttacatgttg gtagcaaaag aaaaaagtta
    13621 tgagttagtg gacaattgtc agcaggatga gtataacatt gtattaagag caagtgaagg
    13681 ggataaaata gcactagagt acattattac taaatacaga aattttgtaa aggcaaaagc
    13741 aaagtcatat tttttaattg gagcagataa agaagatata atacaagaag gaatgatagg
    13801 cctttataag gcagttagag attttgatgg aagtaagacc aattcattta aatgttttgc
    13861 ggagatatgt ataacaagac aaataataac tgctataaaa actgcaacaa gacaaaaaca
    13921 tataccttta aattcatatg tttctttgaa taaaccaata tatgatgaag agtcagacag
    13981 aacactttta gatattatag ctacaagtat agtaacagat ccagaagagc ttataattag
    14041 taaagaagag ttaaaaaata tagaatcaaa aatgaacgaa ttgttaagtg acttggaatt
    14101 agaagtacta gaattatatt taaatggaaa gtcataccag tttatagcgg acaagcttaa
    14161 aagagatgta aagtcaatag ataacgcttt acaaagggtt aagagaaaat tagaaaaaca
    14221 tcttgaaaac agaaatgatt aatagtaaaa tacttaccat aagcttattg tgagcaaaat
    14281 taatttatat cgaaagataa gggaggaaat tcaaaatggc taaagctaaa tacgaaagaa
    14341 caaaacctca tgttaatata gggacaatag gacacgtaga ccacggtaaa actacattaa
    14401 cagcagcaat aacaaaaaca ttatatgaca gatatcaatt aggagaagca gtagatttcg
    14461 caaacataga taaagctcca gaagaaagag aaagaggaat cacaatatca acagcacacg
    14521 ttgagtatga aacaccaaat agacactatg cacacgttga ctgcccaggg catgctgact
    14581 acgttaagaa catgataaca ggagcagcac aaatggacgg agcaatatta gtttgttcag
    14641 caacagatgg accaatgcca caaacaagag agcatatact attatcaaga caagttggag
    14701 taccatatat agtagtattc ttaaacaaat gtgacatggt agatgatgaa gagttattag
    14761 agttagtaga gatggaagta agagatttat taacagaata tgatttccca ggagatgaca
    14821 ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagt gagtggggag
    14881 ataagatagt agaattattc gagcaaatag atgagtatat accagctcca gagagagata
    14941 cagataaacc attcttaatg ccagtagagg acgtattctc aatcacagga agaggaacag
    15001 ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta gaattagtag
    15061 gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc agaaaattat
    15121 tagaccaagc acaagcaggt gataatatag gagcattatt aagaggagta caaagaaacg
    15181 agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac acaaagttta
    15241 cagcagaagt atatgtactt aaaaaagaag aaggtggaag acatacacca ttctttgatg
    15301 gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt aagttaccag
    15361 aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac ttaataaact
    15421 caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga acagtagctt
    15481 caggagttg
//
LOCUS       DAEMHZ010000100        15322 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000100 DAEMHZ010000000
VERSION     DAEMHZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.100"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(282..1172)
                     /gene="metA"
                     /locus_tag="KPK72_002740"
     CDS             complement(282..1172)
                     /gene="metA"
                     /locus_tag="KPK72_002740"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG2489938.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1202..2467)
                     /locus_tag="KPK72_002741"
     CDS             complement(1202..2467)
                     /locus_tag="KPK72_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG2489939.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2577..2679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2733..5390)
                     /locus_tag="KPK72_002742"
     CDS             complement(2733..5390)
                     /locus_tag="KPK72_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2489940.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5518..6261)
                     /gene="yaaA"
                     /locus_tag="KPK72_002743"
     CDS             complement(5518..6261)
                     /gene="yaaA"
                     /locus_tag="KPK72_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG2489941.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFAIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGVMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSTNSEYIFVKE"
     gene            complement(6271..6735)
                     /gene="bcp"
                     /locus_tag="KPK72_002744"
     CDS             complement(6271..6735)
                     /gene="bcp"
                     /locus_tag="KPK72_002744"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG2489942.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(7087..8784)
                     /gene="ade"
                     /locus_tag="KPK72_002745"
     CDS             complement(7087..8784)
                     /gene="ade"
                     /locus_tag="KPK72_002745"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG2489943.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(9090..10244)
                     /locus_tag="KPK72_002746"
     CDS             complement(9090..10244)
                     /locus_tag="KPK72_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG2489944.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(10489..11328)
                     /locus_tag="KPK72_002747"
     CDS             complement(10489..11328)
                     /locus_tag="KPK72_002747"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG2489945.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(11341..11937)
                     /locus_tag="KPK72_002748"
     CDS             complement(11341..11937)
                     /locus_tag="KPK72_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2489946.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(11988..12638)
                     /locus_tag="KPK72_002749"
     CDS             complement(11988..12638)
                     /locus_tag="KPK72_002749"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG2489947.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVISKINL"
     gene            complement(12684..13937)
                     /locus_tag="KPK72_002750"
     CDS             complement(12684..13937)
                     /locus_tag="KPK72_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2489948.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEELALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAVPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(13961..14602)
                     /locus_tag="KPK72_002751"
     CDS             complement(13961..14602)
                     /locus_tag="KPK72_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2489949.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(14804..15217)
                     /locus_tag="KPK72_002752"
     CDS             complement(14804..15217)
                     /locus_tag="KPK72_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG2489950.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
BASE COUNT         4800 a         2751 c         1635 g         6136 t
ORIGIN      
        1 aaatctatat atattttaca ctgatttctc cctttttaat ctcttttaaa agttaaaata
       61 caaatttatt gatactataa aggtttagtc tcccattatc gcaccatata taaaattaaa
      121 gccacctcta tctacataca cctaaagtga ttttcttaaa atataaaact actattataa
      181 ggtcaaattt taaaaaaata aggtataaag tatcattaag attaacttta tacctataaa
      241 tttctatatt tttttagtta acatttatat actcttaaaa tttattgtat caaatagttt
      301 tttatccaat ttctaaacaa caattctgaa tgttttttcc attttacagt tggctcttct
      361 gaagggtcat cattaatata gtaattttga ggtatagata ttttatctcc tttatttata
      421 tccctcttat attctttatc aagagtgtat ttgtcatact cgctatgacc cattacgaac
      481 acatctcttg aatttgtaat tatataggga ccagcttctt tagaatgact cacaatgctc
      541 aactctttat tctctttcaa agcttcaata tttacagtag tatgtcttga atgtggagca
      601 aaaaattcat tttcaaatcc atctactatt ttactatctt tatctacctt atgcttaaat
      661 actccaaaac atttttgact tagtggaagt ttttttacat tgtagtattt gtaaagactt
      721 gcttgagctg cccaacatat atataatgta gatttagttt ttctcttaga ataatccata
      781 atttcttcta gttcattcca ataatcaact tcttcaaact ccatttgttc aactggagct
      841 cctgttatta taaagccatc aaaatactct cctttggctt catcaaatgt tttataaaat
      901 tctttaacat attcattaca acttctttta ctttctcttg ttgaaatttt aataaattcc
      961 acagacacgt tgaatcctgt tttatctatc cttcttagta aatctaattc tgtatctatc
     1021 tttataggca ttagatttag tatagctatc ttagtgtcta tgttttcttc atactttaat
     1081 tcctttttaa aatcttcttt ataaattaca tctattcctt catctaataa tttattaata
     1141 acaggtaatc cctttggtag tattaaagcc atatcttttc acttctcttt ctcttagaat
     1201 attagaaatt taaagcttga tttaaatcag ctattaaatc ctctacattt tctattccaa
     1261 cagataatct tattagtgaa ggcaatactc cagatgcaat taattgttcc tctgttaatt
     1321 gtctatgagt agttgaagct ggatgtataa cacaagttct tacatctgaa acatgtgtaa
     1381 ccaaagatgc tatctgtaat ttttccacaa attttttggc attttctaac ccacctctta
     1441 ctccaaatga taaaacacca ctacatcctc ttgataaata ctttttggca ttctcataat
     1501 acttgttatc ttcaagtcct gggtaattaa tccaatctac attttcatgt ttttctaaaa
     1561 atctagctat tttaagtgca ttttcactat gtctctccat tctaagatgt agtgtttcaa
     1621 catttaaatt agtaagatac gcattgaatg ggcttaaaca atttccatag tctctaagca
     1681 actgaactct tgctttagtt gcatatgcgg catttccaaa tttttgagta tagcttatac
     1741 catgatatgt tgggtctggc tcaactaatt ctggaaattt tccattatcc cagttaaagt
     1801 ttccaccatc cactataatt cctccaacac ttgaagcatg cccatctaaa tattttgtgg
     1861 tagaatgagt aactatattt gctccatact ttaaagggtt acaaagcact ggagatgcta
     1921 atgaattatc aacaataaat ggaacattaa ttctcttagc tacatttgct attttttcaa
     1981 aatctatgac ttcaagagtt ggatttgcaa gtgtttctgc aaacacaacc ttagtatttt
     2041 cctttgcaag ttctactatc tcatcttcac ttgaatctaa gtcaaacgat attaaatcta
     2101 taccaaattt tttaagacta ggtcctagta aattgaatgt tcctccatat acttttgaag
     2161 aacaaagtat actatctcct gatttacata tatttaaaac tgccaacata tttgcagatt
     2221 gccctgatga tacagctaca gaagatactc caccctctag taaacttatt ttttcttcaa
     2281 aagcttgaat agtaggattg cttattcttg aatatatatg tccatcaact tttaaatcaa
     2341 ataattcagc aagttggtct atactgctat atttaaaagt tgtactttga tataaaggta
     2401 gtactcttgg ttctccatta cctggtttat aatttccttg cacacatatt gtttctttat
     2461 tatacattct tgtatccccc ttagatttta gtaaaaaaaa ttaaactatt taccagatga
     2521 gagttaaaat aaataaagcc tgagtatata actcaggctc tgtataagct taattatact
     2581 catctctcag ctttcgccgc aggatttagc accaatgtat ataaatatac tggttgccgg
     2641 gtttcatagg gccagtccct ccacctctct ggataagatt tatttaattt ttctatgttt
     2701 tatatcttat atatttttcc taattttgtc aactattttt ttaatttttt tcttttaata
     2761 attttcccaa cctctacttg aattacagac attatggcta aaacaatcgt tataagccac
     2821 tctaagttat ttaaaactgt taagttaaaa gcatttctaa aaacaggtac aaatagtatt
     2881 actgctatta aaaatgcaga agttaaaaat gagattaaaa gaaatggatt tttaccatta
     2941 tttctattaa acattgaatc tgtatttgaa cgctgactaa atgctcgtaa catctgtgaa
     3001 aatgctaata cacaaaatgc cattgtttga cctacagaat gattttctat attaagacca
     3061 attttatatg ctaaaatagt cattattgcc acaaatatac cttgggtaac aactctttgt
     3121 ataagatttt tttcaaacaa agtggttgat ttcactggct ggtgcttcat tatatttctg
     3181 cttggtgggt caactccaag agcaagggct ggaagcgtag cagttgcaag gtttacccat
     3241 agaatatgaa cagcaagaag cggagtatcc caatttaata atattgctac aaacaacgta
     3301 agtatctctg ctatattacc aacaagcaaa aattgtataa ctttttgtat gtttctatat
     3361 acacgtctac cttcttttat tgcataagca attgttgtaa aactatcgtc cattaaaatc
     3421 atgtcagaag aatcttttgc aacatctgta ccacttatac ccatggctat accaatatca
     3481 gcagacttta aggcaggaga gtcatttaca ccatccccag tcattgctgg tacttctcca
     3541 attcttttta gagattgtat aattctaagt ttatcaaatg gggatacacg agcaaatact
     3601 gtagtatttt taacagcttc atctagttca tcatcactca aattatcaag ttcatcacca
     3661 gatatcacag tattttcttc actccatatt cctaactcat gagcgattgt tgtggcagta
     3721 actttatggt cacctgttat cattatggtt cttatacctg cttctttaca agtacttact
     3781 gcatcaatta cctcagttct tggtgggtca atcataccaa ccatacctat aaatgttaaa
     3841 tcatattcta tattttcact atcttctttt ggtatagatg aaatctcacg cttagcaaaa
     3901 ccaagtacac gcaatgcatc tttagacatg ttaagacata attcgtgtat attcttttta
     3961 tccacttcag taatgtttct ttcacctttt gaagttaata ttttagtaca taggtcaaga
     4021 atttcgtcta ctgctccttt agtataagca ataatctttt catctatatt atgaacagtt
     4081 gtcatacatt ttctatctga atcaaaaggc tgttcaaata cacgtggata ttcttcttca
     4141 agagcttctt gatctattcc aaattttttg gccataaaaa ttaatgcgcc ctctgttggg
     4201 tcacctaata tttcatctgt tttatctgga ttaatacatg catcattaca aagtgcacca
     4261 gcataaacaa tctctttata cactaatgga tgtttttcag atgcagtatc tattgatgtc
     4321 acttttccag attcaaaatc tccattgaca gcaatatgtg taacagtcat tttatttaag
     4381 gttaatgtac cagttttatc acaacatatt actgtagctc ctcctaatgt ttcaacagca
     4441 ggtaattttc taattaaagc atttctttta gccatacgct gtacgcctaa agccattaca
     4501 atagtagctg ttgcaggtaa tccctctgga ataattgata tggcaaggga aattgcaatc
     4561 ataaacattg gcaagagagg tctctgataa aatgcaccta ttgcaaatat aacaacgcat
     4621 acaataatac ctatgacact taatgtcttt ccaacagtat taagtttacg ttttatagga
     4681 gtatcaaaat catcttgtga atctaaaaga tgtgctatgt tacccacttc tgtatccata
     4741 ccagttgcta ctacaacacc tacacctcta ccatatgtga ctattgaaga tgtatacgcc
     4801 atattgctac ggtctcctaa tacacattct ttaggtaaga ttgtatctgc atctttttct
     4861 gatggaacag attctcctgt taaagaagcc tcttgaattt ttaaacttga tgtttctatt
     4921 aaacgaaggt ctgctggaac catagctcca tcttctaaaa aaacaatatc tcctactaca
     4981 agattacttg ctggaataac attttcttca ccatcacgca taacacgagc tgtaggtgaa
     5041 ttcatatttt ttaatgcttc taatgatgat tctgcttttt tctcttgaac aattccaata
     5101 actgcattta ctgttacaat tattagtatt acaaaagctt ctgtaaattc gccaaatatc
     5161 aaagataata ttgctgctcc tattagtatc ataaccatag catctgtaat ttgactccaa
     5221 agcatttgta aagttgttct aggtggtttt tttctaagtt catttaaacc attctcttta
     5281 agccgttttt ccgcctcggt atcggtgagc ccctcctctg atgaatctag actttctaaa
     5341 atttcatgcg ttgctcttga atgccaaggc atctgtttgt gattttccat tttggaagac
     5401 cactcctttc tttttttaat ttaagaataa ttatatttta aaatcaagtt caattcaata
     5461 cacactaagt atattttacc taaatttaac aatgccattt aatatttaac aaaacttcta
     5521 ctcttttaca aatatgtatt cactattggt agataagttt tctttatact tataaccttc
     5581 taaattaaac tttttcagtt cttcaatatc tcttatccta ttttttacaa tgtattttgt
     5641 catcacacct ctagctttct ttgcttgtgt agattccact ttcaatatgc catttttatt
     5701 tactttaaaa gtacatgtaa tatatgtatc actatcttta ataaattttt ctatggattt
     5761 gctgtattca tttgatgaaa gattaacgat agctgttttc tctttgatta gttctttata
     5821 tatacgattt ccccaatatt cgtataaatt cttaaactca cctactcgta attttgtctg
     5881 catttctaat ctatattcat atatactatc atatggtttt acaacaccat acaatccact
     5941 tatgattcga atacagctat ttgcaaactc ttcatctttc cttgtaaaat tttctgcttc
     6001 tatattttta tattgtattc catcataagt taagattgca gggtttccta atctatcaaa
     6061 tttaatgctt tcaaatctat ttttatttaa tacagctaat ttttcattaa ttttcatttt
     6121 atttttaaaa tcttctatag caaatgtctt tatattttcc actatttctt ttgtgttatc
     6181 tataaaacaa ggcaaactca aatctctatc aaatacttct atattcttca tatttttagc
     6241 tggtgataac attataatca taaattctta ttactcctgt ttttccaaat attcaagaat
     6301 ctcttgtgca tttgtgtctg gttttgcttt ttcaaagact ttttcaataa ttccattttc
     6361 atctataata tatgtcgcac gtacaactcc caatgcagtt tttccataca tttttttctc
     6421 tttccataca tcaaaagctt ttatagcaac taagtcaggg tcagaaagta aaataaaagg
     6481 aagttcatgc ttttctgcaa atttttgatg tgattttata ctatctttac ttatcccaat
     6541 aacttgtata tcttcctttt taaaaccatc atatgcattt ctaaaagcac aagcttgacg
     6601 tgtacatcct ggtgtattat ctttaggata aaaatatact actacttttt tacctttaaa
     6661 atctgacata cttaccttat ttccatcttt atcttctagt gtaaattctg gtgcttttgt
     6721 tcctatactc aacataaata tctctccttt tgtttttatt aataatttta tactatgctt
     6781 atattattac cctataaatc tatatataaa taaaatattt tattttttat gtataaagtt
     6841 tcataaatat ataattttca tgtatttata tgttaaaata gactgaaaat agttttatta
     6901 agatatctat gcttaaaaca aggttaaatg atagattaat taaaataagg atatctaaaa
     6961 ataaaatttt tagttcatga gatgctcttt tttgtatgaa atcaaatata ttttcatcat
     7021 ttcaacaata aaaagagact tatccctatt ttgagaaagc ctcatttatt attactttta
     7081 tattatctat ataaatttaa attcctcaac atcaaataga cctaaatctg taagtcttat
     7141 ttgtggaaca actggaagtg ccataaaaga aagtgtaata aaagggtcta catactctgg
     7201 aactcccatt tttcttgcta tctccaacat attatcagta atttcttgta cttcttcagc
     7261 tgtaaaagta ctaattaatc cagctacttg aagacttagg tgtgcaagaa cttttccatt
     7321 tgatacaatt acataaccac cttgtatttc ctttaagtaa tttatagcta ctagaatatc
     7381 atcatcatta tcccctgcaa ctataatatt atgagaatca tgggccacag tagtagcaat
     7441 tgctccattt tttatgccaa atccttttag tggagcaatt gctacatttc cagtcattct
     7501 atgtctctca acaacacaaa gttttgaata ctctttattt ggaacaaaat atccatctgc
     7561 acatggtaaa gattcatata ccttatttgt caaaatttga taaggtacaa tctccattac
     7621 ataatttttt tcacttactt ttaactgtat tttttcttta ttaatatatt taaacttgac
     7681 tgtattaagc aattctttat ctttaatctc ataattgtaa ttagaaaaca tctcttcgtt
     7741 taccaaatta ccatctttat atacactatc cacttccatt ttgtctaaat cattcaatac
     7801 aacaatatct gccttataac ccgcacctat tgctccaatt ttccttaacc catatgcttt
     7861 tgcagaatta taagttgcaa cttttatagc cctaacaggt tccataccta aatcaatcgc
     7921 acattttata ttccatctta tgtgaccttg tttttctata tcatctaagt gcttatcatc
     7981 tgtgcaaaac ataaattctt caattggcaa gttatgcttt agcataccac ttactataga
     8041 ctttaaattt tttgctgcgc ttccttctct tattaaaatt tttaatcctg ctctcaattt
     8101 ttcatatact tcatcaaaag ttatacattc atggtcattt tctattcctg cacatacata
     8161 tgtctgaagt gatttaccat ttatattagg tgcatgtcca tctacaactt tatttttaaa
     8221 aagctctagt ttatccaaga tttcattttc agaatttata acatcattaa aacacattac
     8281 ctctcctaaa cctaaaattc taggattatt tacataactc tccatgtctt ttgcactaaa
     8341 tttaccaact ccattagttt caaaacttgt tgcaggtacg gatgaaggca ccataatata
     8401 tacattaagt ggtatatctt ttgtcgattc taataaaaaa tctattgcac tagctccttt
     8461 tacattgacc aactcatgtg ggtcggctat gatagttgtc gtacctgatt ttaataattt
     8521 tttcgataat tcaattggca ttaccattgt agattcaata tgcatatgtg catcaataaa
     8581 gccaggtgat atatatttgt tgctacaatc aatttctaca ttgcccttgt aatcgccaat
     8641 accaacgata gtgtctccta ctattgctac atctcctgtt attaactctt ctgtaaatac
     8701 atttatgatt gtcccatttt ttagtacaat gtcagctttt tctcttccac ttgaaacatc
     8761 acattgtctt tttaaattac ccatttttat aacccctcct aagtttttta tatcaatata
     8821 ctgactgtat tacgtattat agtttaattt attaatatat attatatatg agttttgata
     8881 gaaagtatca cttgaaattt gttccataat tcctttaatt aaatagagag aacatgagga
     8941 ataatattat ctcccatgtt ctcattaata tttaagcaat aatttaattc atattgaatt
     9001 ttttattttt atatttaatc ttcactattc ttataaataa atttatgtgc tttattattt
     9061 ttttaatact tctttattcg taattatatt taacctaaaa atgcataaac tatcatacct
     9121 acaagtattg ctggtatagt agtaataatc atagcccata aagaagattt tttatccatt
     9181 gctaaaagtg ggaaaagtgc atctccatct tgagaaatag agttggctat cactgcacca
     9241 agtggtatag ttcccttcaa ataaaatgac ataagcacaa tttgtatccc acaacctgga
     9301 ataagtccaa gacctgcacc tataaataca cttattacac ctgttgataa tactagttta
     9361 actaagtatt catctccacc taaaattaat attatcaaat cgtaaataag atatgcaata
     9421 aaaatccatg taattacaaa tgatatttct ccaacagaat ggataagcat ttctcgcaaa
     9481 gaaatctttt tattttcttc ctctttttga ttagaatttt taaatacctt tttaaaacac
     9541 cacatataaa ttattgaaaa taatattcca atcactgcta ttacttcttc caatgaatgt
     9601 aacttctcaa ttataggaag attaagtcca gagtgagcaa gtgacataaa tataaaaccg
     9661 attactataa ttcctatata aattttatat ccctttccat gtgttattat gaacgcaagt
     9721 ctatttgtat ttccatgaga tttagctaga gtgtctaaaa ttatttcatc atgattatgt
     9781 tgttctttat tttctttaga gttattttta cttatatcat atttatctga ttttctacgg
     9841 ttttttaacc ctagtttttc ttctagttta aagtaatcca ctaattgtcc agttattatt
     9901 cctgtaattg tactaactat agtgacaaaa aaatacattt taatatttga tgaaataagt
     9961 acaaatgctg catctcccat actagctatt aaacttgcaa ttattgctcc aaagcttaat
    10021 tttccattta tatacaatgg cattattgcc aaacttccgc cacaacctgg tatagctcca
    10081 attaaagcac ctattaatgg ttgaaatttt ctgttttttg atatgatatt agtaaaattt
    10141 ccactagttt tataattaat atatccaaat aacaaaataa aaaatccaat catagaacct
    10201 acatgtaaaa acgcctcttc agaagcggat atcaacattt ccattaattt acctcctaga
    10261 ctaattttgc aacaattata ttatattcta ttttctattg ataatcaata tcaatatttc
    10321 ttttgaattt ctaatattaa aaagtgcgca tcttatactc ttagtttttc atcttaagat
    10381 acacactttt tgatattagg ttatctattt accacatgta atatgaaatc aaaggtggat
    10441 attatgataa attataacaa tgtccaccta tttttattac ctatcaaatt ataaattttc
    10501 aaggaatgtt acaacttctt ctattatcca attaggtgta gatgcccctg ctgtaatacc
    10561 caaattttta tattttttaa gcgtatttac atccaattca tctttagtct ctattgcaaa
    10621 agttggtact aagtcttcac aaacctttac taacttttga gtatttgaac tgtgtttacc
    10681 tcctataact atcatacaat caacttcttt agctaactct ttagctgatt cttgtcttgt
    10741 ttttgtagct gaacaaattg tatcattaaa tactatttct tctaatttat ctgatagttt
    10801 attaactata cttatataca actctggatt tattgtagtc tgtgcaacaa gacaatattt
    10861 tttggagtta tcaaattcca tattttccat ttgttctaag gtctttatta ttatagctga
    10921 attttcacac catccattta tacctataac ttctggatgc ttcatatctc caataactat
    10981 tatttcatag cctttattat gatactcact tactactgta tgtattttct ttacaaatgg
    11041 acatgtagta tccacaattt ttaatttttt atctttggct tccttatata tattttcagg
    11101 aactccatga gaccttatta tcatattttc atgatttggt attgtatcaa tttcattcac
    11161 agtcttaaga cctttttctt catattttgc tactgcttgc ttgttatgaa taagaggacc
    11221 taaagcatat atgccagaat catttttttc aacttcatcc catgccattt tcattgctct
    11281 ttttactcca aaacaaaagc cagcattttt tgctattttt atgttcataa ctttactccc
    11341 ttattttgaa tatttaaagt aattttgttt tatcacttct aaaatctctt gtgatatcct
    11401 ttcatggtca tcattactca atttttcctt aaaatgttct tcaaatgata ttggttctcc
    11461 aatatataca attactttac taaaaagttt atatttagat attattgata ctggaacaac
    11521 tttagattta ctttttattg taaatacaga aagacctgcc ttagccttac caaaagaatc
    11581 tccttttatt ctagtaccct ctggaaatat tcctaataca taaccatctc taactgacct
    11641 taaaatagtt tttattgtag atacatctgg tttttctcta tttataggca taacatgcaa
    11701 tttttttaat ataaatccca atggttttac tttaaacaat tctttctttg caattgcagc
    11761 tatttctcta ttttctatgg cagcagccaa gaatataggg tcaagattcg atttatgatt
    11821 tgatgctatt actatatttc ctctatctgg aatattttct gcacctataa cttcatattt
    11881 aaaaaatatt ttagaaaaac ccttaaaaat atttattaca aatctataaa aattcacgtt
    11941 tatctccctt caacactaca tttattttca ttatgatgca taaaaaacta aagatttatt
    12001 ttagatataa cttcttctac aacttcttct atagtttttc ctgtagtatc tatttctagt
    12061 gcatcattag cttttaccaa tggtgcaaat tctctatttg aatcaatttc atctcgtctt
    12121 attatatcct caattacttc ttctaaagta gtattatatc ctttattaca aagttcttta
    12181 tagcgtctat ttcctctttc ttctggagta gctactaaaa agaatttgta atctgcatct
    12241 gggaaaacat atgaacctat atctctacca tctagtatta ctgaactttt cattcctatt
    12301 tttcgttgaa cttcaaccat caactgtctt acttctttta tttttgctac attagaaaca
    12361 ttattgctta cttctattgt tcttatttct tcatttataa cttctttgtc caaatatata
    12421 ttgttatctt taaaatctat atccgaattt tcagctattt gaataacttc tttttcatta
    12481 ttgacatcaa caccactttt taaacactta taagtaactg ctctgtacat agcacctgta
    12541 tctatataat ttatatttag cttttttgct actatcttgg ctattgtact ttttccagct
    12601 cctgctggac catctacagc tataactaaa ttacccatat atacacctct atttattcat
    12661 atttaattat taaaaaatta tatttataca cttatacccg caagatatcc agttgaaaat
    12721 gctatttgta aattatatcc accagtttct gcatctatat caattacttc acctgtaaaa
    12781 aataagttct taactaattt agattccata gtagaagaat taatttcttt aactgaaata
    12841 ccaccagaag taaccatacc agtgtttata cctgtagtcc cattacaagt tagcttcata
    12901 ttttttatat actcaataat tctaatttca tctgccttag acaaatcact tgccttttta
    12961 tcaactaaag ataatagaga aaaaatctct tttaagaaat tttgaggtag tatttgctta
    13021 agattattta gaacattttt atttggaact gctctgatta tactagagat ttcatcagta
    13081 cttaaatttg gtaagaaatc tatatttaat tctacctttt ccttttcaat aatcttgtta
    13141 atataagaag acattataag aacacaaggt cctgttatcc caaaatgggc aaatagcatg
    13201 tctccagatt ttgatatttt ccttttctta atcttacaag atatttccac attcttcatg
    13261 gatattcctt gtaaatcttt aatccatttt tcctctattg taagtggaac taaagcagga
    13321 tatagtttgt ttaatgtatg tccatgcttt ttaagaatct tatacattga accatctgaa
    13381 cctgtctttg aataactcac tcctccagtt gatataataa ccttgtctcc tagtatctta
    13441 tctccattat ctaaaataat cccttttatt agatactttg acttgtcctt attagaatca
    13501 tcttttagag caagttcttc agttataata tctataactt tcttattata cattatcttt
    13561 acattgtgat tgagtaaatc tttatttaat acttctatta cttctataga tttatcattt
    13621 ttagtgtata ctttgtggtc attttcttct tcaatcttat attccaaatt tctactttca
    13681 aaatacgaaa taaggtcttt atttgtaaat gtatagaaac tactatagag aaatttttta
    13741 ttactgacaa ctttatcaaa aaaatcttct atctctctat tattggtaaa attgcatcta
    13801 ccgccaccag ttgccctaag ttttcttcct aattctccat ttctctcaac taatattact
    13861 tcattattgt tttttgaagc tgacaaagct gccatcattc cagaagctcc tccaccaact
    13921 actataactt tactcataaa tttctcctta taattgattc ctactccagt gcagaattat
    13981 ttatctttac atgatttttt atatgacttg tatcattcat ttttactata acaggtccat
    14041 aatctttaaa ttctactata tttaaagcag tattatcttg cttaattcta tatatatttt
    14101 ccatacctga ttctaaaaat gacaacaaca taaccctcaa tgtaattgaa tgtgtaacaa
    14161 gaattatatt tttattatca tatttttcat ttagttcctt tataaaagca tctactcttt
    14221 cctttataat cttaagagtt tcgccttctg gtatatttac taagtgtggt tcatttctcc
    14281 atgttgcata tatatctgaa taatcttttt gaatttcttt tattaaaagg ccttcccaaa
    14341 ctccgaatcc catttctcta agtgcctcag ttttttgaac ttcaatattg agcttatctc
    14401 ctaaaatttg agctgtttgc attgctctcc caaggtcgct actaaaaata taatcaattg
    14461 aatacttgcc aatatcttca gaaagttctt ttgcctgttc tataccttga ggcgtaaggt
    14521 cagaattacc atgaccttgt gtttttccca atatattcca atccgtttgt ccatgtctta
    14581 ctatataaaa agtatttccc atatgtcctc ctaatatatg cctatttttt attaaatata
    14641 aattatcact tctcaaaact taattatatc atataatact ttactttgtt tctattttat
    14701 aatctaatct aacatattat ttacaataaa tctcatttgt atttctgcaa attattgtac
    14761 tgttaaagaa tttttctaaa tttataaaca tatttctctt atattattta tatacttctt
    14821 caaatctgta ttcttgaatt gaattgacat acttatctgt atctactttt tctaaaaaca
    14881 tatccaaagg tcttgcaaat attcctgtat catcatataa agtcttgtat aaaactaaat
    14941 cattagtatc ttcttctttt ttatgataaa agtttccatc taaatcccta taaacataga
    15001 tatcttcttc tctttcagta tgtctaataa ctaatttata tttatttctg ttttggatta
    15061 aattttcttt accatatatt tcacatatgt tttctatatc atcaatagcc ttgctaagac
    15121 ccattgtagc ataatatttc cctttgaaat gtttatagat acaaggatat tttaactttc
    15181 tttcactgat acctaagtta ttctttaatt caatcatttt attataatct cccatatctc
    15241 gtccctttct tttaaaaagt attttattct gaaataagtt acttgtttta tattttatca
    15301 taatttaaaa tttattaata at
//
LOCUS       DAEMHZ010000101        15236 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000101 DAEMHZ010000000
VERSION     DAEMHZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.101"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(214..525)
                     /locus_tag="KPK72_002753"
     CDS             complement(214..525)
                     /locus_tag="KPK72_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489951.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(581..1174)
                     /locus_tag="KPK72_002754"
     CDS             complement(581..1174)
                     /locus_tag="KPK72_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2489952.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            1738..1968
                     /locus_tag="KPK72_002755"
     CDS             1738..1968
                     /locus_tag="KPK72_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489953.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            2025..2675
                     /locus_tag="KPK72_002756"
     CDS             2025..2675
                     /locus_tag="KPK72_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2489954.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIVIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            2889..3560
                     /locus_tag="KPK72_002757"
     CDS             2889..3560
                     /locus_tag="KPK72_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2489955.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            3557..4687
                     /locus_tag="KPK72_002758"
     CDS             3557..4687
                     /locus_tag="KPK72_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2489956.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            5130..5975
                     /locus_tag="KPK72_002759"
     CDS             5130..5975
                     /locus_tag="KPK72_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2489957.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            5975..7003
                     /locus_tag="KPK72_002760"
     CDS             5975..7003
                     /locus_tag="KPK72_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489958.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            7006..8019
                     /locus_tag="KPK72_002761"
     CDS             7006..8019
                     /locus_tag="KPK72_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2489959.1"
                     /translation="MIGLHYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TRGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(8536..9786)
                     /gene="cme"
                     /locus_tag="KPK72_002762"
     CDS             complement(8536..9786)
                     /gene="cme"
                     /locus_tag="KPK72_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG2489960.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGRGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(9872..10618)
                     /locus_tag="KPK72_002763"
     CDS             complement(9872..10618)
                     /locus_tag="KPK72_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2489961.1"
                     /translation="MDTYTTSKVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVTQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(10733..11755)
                     /locus_tag="KPK72_002764"
     CDS             complement(10733..11755)
                     /locus_tag="KPK72_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG2489962.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSTRDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(11755..14175)
                     /locus_tag="KPK72_002765"
     CDS             complement(11755..14175)
                     /locus_tag="KPK72_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2489963.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAGHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(14261..15181)
                     /locus_tag="KPK72_002766"
     CDS             complement(14261..15181)
                     /locus_tag="KPK72_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2489964.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKK
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         5378 a         2005 c         2361 g         5492 t
ORIGIN      
        1 gaagtataat aaactaattt aagaaatata gaaaattaat ttaaagaaat acaatacatt
       61 aatttttaag taacattaaa ttaatgtaag taaaataatg ttcatatgaa tatttaaagg
      121 tgcattttct gaagctaagt atatgttgta gagtgttatg tctctctatc ctgtgaagtt
      181 taatagctca agaaaatgca ctatttatga tgattatttt gttttatttc caattttgga
      241 aataactatt ctaattaaat ctaaaattat accataaata agaaaagaaa gtatagatgc
      301 tatgtaccaa taaatcgaaa ttgaccctgt aattgttcta catgggattt gcatacctga
      361 atccataaaa acaaaaaaac tatcaactct ggaaataata gacaaaatag gatttaaacc
      421 tattagaaca atactttttg agtcttgacc aatttgatgc atataaatct caaataatgc
      481 aattaaaaaa aaccaagatg taaaagattt aataattttt ttcatcaaaa tacctcacat
      541 aatataaatt ctgtttgcac tatttgctta ctacaactat ttatctccat tcagtcacat
      601 cacttaattg taatctaggt cttcttgatg gttcttcatc aggataacct aaagatattg
      661 ctgccactaa ttgactatct gtatttaacc attcacataa ttcacgatat gcaaagtata
      721 catcacaaat ccaaagacta cctagaccta attccacagc tgtaagcgac atattctgaa
      781 ttgcagctcc aattgattgc atatttgcca tttcatagaa tcgttcttca ggggatagct
      841 tttctaaagg tgatttacct aatatattta aaataaaaat tgttactgga gcttgtttca
      901 tgatttcaac tgtgtaattt gcaccagcga tatgttgacg acttcctgga agaagcccat
      961 tatcattaat ttcattttga atacctttgc tcatggcttt aagcatagat tctttttcct
     1021 tttcagtgat gacaacaaaa cgccatggtt gcctgttttt agatgaaggt gcatttattc
     1081 cagcttcaat tattttctct atagtttcat gtgaaatgct ttgattttta tattttctga
     1141 tacttcgtct ttttgatata gaatcactta tcattttaag taacctccgt ttattttttg
     1201 gaaaaatcta tttaattttt attttctatt ttaattattt tagcattaat cagaaaaaaa
     1261 gtcattgatt ttagtataca attattaatg attatattaa gaattgtatc aaaaagtatg
     1321 tgattatact aagggattat attaaacatt atatttaaaa ttgtatgaaa ggttgtattt
     1381 aatattatat taagagagat atatattagg atttatatat attaagattt attattatat
     1441 tgagccttat attaaattaa ggactataat ttgaaaataa gctataaaaa cttaatattg
     1501 ttttgttcaa agataaaaaa agaatacttt aggtgatttt tattaaaaat taagagtgat
     1561 aagattatat actttataaa tgtaaatatg acagataaaa atgataaata taataatata
     1621 tatatttaca ttaggagttt tctatactat aattatttaa aactaaatat aaatgttgga
     1681 attatttagg acttgaaatt gtctaaaaat aaatataagt gatgaagagg tgatgtgatg
     1741 agattagata tggatagtat gttatatgta ctaatttata tgacattatt ggtatctatc
     1801 attggaagta tattttattt ttgtaaagac ctcttaaatc ataaaattaa gtatgtttta
     1861 ataagagaca tatttgtaaa tagaattaca tattctgaag aacggttgcc taaacagtat
     1921 atagtttttc agaaatatga tatttggagg tattgtagtt tgttttaatt tgaaatgaat
     1981 ctattataaa ttctaaagtt taaaaataaa aaggagagaa taaaatggat ttcatttcaa
     2041 gtatattgca aggattatta agtatattat ttaattttac aggtgatttt ggaatttcta
     2101 ttgtacttat aactttaata gttaaattag tacttatgcc actatcatta aaacaaagat
     2161 tttctgtaaa aaaacagcaa gaattagctg aaaaaatgga acatataaaa gaaaagtata
     2221 agaatgatac taaagaattg gaaaagcaaa tacaagtaag ttctgtagaa agtatgaaga
     2281 gtatgctggg atgtcttata gttttggttc aaatgccagt agtatatgct ttatatcatg
     2341 tatgtttaga tatgacaaag gaatttacaa caattgtaat tccatggatt gctaatttag
     2401 gtacatttga taatttgttt atattacctt gtatatatac attaattatg ttagcttcaa
     2461 atttagtaaa ttatatacca tatttaaaaa taaattctca agtaaagttt aacaaacaaa
     2521 tggctgtggt gactaccata acaagtctta tactaactgt tagaactcca gtagccatag
     2581 gtctgtactt tataacaagt gcaatttatt cattaattga agatatttgt tttagaatat
     2641 attttaatcg aaaaaataga atagtgacaa aataatataa ttattattag aaatatttta
     2701 gcctgctaac atagaaaaag ttagtaggct atttttatct atgatatcaa ttccattaag
     2761 aatagtctaa aaaatactat caaaatgcta taaaaaatac tatcaaaata ctataaaaaa
     2821 atagttgaaa aataaataaa ttaatataaa ctaaaataaa gcctacaaaa aagaggtgat
     2881 aaatagaaat ggctaaaata ctaattgtag aagatgaaaa aaggatgcaa agtattattg
     2941 tagaatatat gcaaaaggga ggatatactt gtataactgc tgatgatgga gtcgaggcac
     3001 ttacaatatt aaagagcaac aatatagatt taatgatatt ggatattatg atgccgtatt
     3061 tagatggatt ttcagtgtgt agagtttcac gtgaaatgac taatatacct attattatat
     3121 tgacagctaa aggtgaagag gaggacaaat taaagggtta tgaatatggc gcagatgatt
     3181 atataacaaa accatttagc cccaaagtac ttttagcaaa agttaatgcc ttgttacgta
     3241 gatacactac tgatattcca aaaaattcat tgagtttagg aaagattttt attatggtgg
     3301 catcaaggca agtgtatgta gaagataaat tgattgattt aacatataaa gaatttgaat
     3361 tattgcgttt atttatggaa aaccctaatc aagttttttc cagagagaaa cttttaaatt
     3421 gtatatgggg atatgatttt gaaggaaaca cacgaactgt agatacacat ataaaaacct
     3481 tacgcaaaaa gcttggtagt gaaggacacc atatagttac attaattcgt tcaggatata
     3541 agtttgaggt taaggaatga atagtttaag aaagattata gaaaaactga aaaatgtaga
     3601 tatttcaaat tggattaaac aaaattttgg actatattca gttagaaaaa aagtgttttt
     3661 actttctaaa ttatcaggag cgttaattat attattttat ctggttgttg aagaattttc
     3721 gataaactct aaatttgaat tttggatttg gtttataata ctggttatat ttattatgag
     3781 tattgatttc ttattgggaa aatttatttc agaaccaatc acatctataa ataaatctgc
     3841 aaaaagtatg tcccaacttg atttttctaa tccatgtact gtaaatacta atgatgaatt
     3901 tggagaactt tctaggagcc tgaatacaat gtctacaaat ctacaacagg cattatcaga
     3961 cctagaaagt gcaaatattc aacttgaaaa ggatgtaaat aaggaaagaa tgctattgga
     4021 acaaagaaag gagttggtag atacaatttc acatgaaatg aagactccac ttggcattat
     4081 acgtgcgtat acagaggggc ttatggatga agttgatgaa gaaaaaaaga aaaattatat
     4141 gaatgttata atagaagaaa ctgatagaat gaacaatatg attgtatcat tattagactt
     4201 atctgcattg gaagcaggag tttctaaact taacccagag cgttttgatt ttatagagtt
     4261 tgttgaaact gtggcaggtc gtcttttaat tgatatacca gatgccaatt tttattttac
     4321 atatgatttg ccagagtatg aagtttttgt agtggcagat aaaatgagaa tggaacaagt
     4381 agtagagaat ctagttatta atgcaaagaa gcatgttgta tataatggta atttagattt
     4441 atcgattaca tgtgaaaatg gcttgctttt atttgaaatt tataatgatg gtaggtttat
     4501 agagcaagat gagatttcaa agatttggtc taagttttat cgtagtgttc aatctcagcg
     4561 tacaggaggt tcaggtctag ggcttgcaat tgtttcacaa atacttacaa tgcaagggtt
     4621 agaatatgga gtgaataatc gtgatagagg agtagagttt tattttatga ttccaattga
     4681 tgaataattt agtattaatt tataaattat aagtatctga tgtaaaatat ttttatttgt
     4741 atacgtaagc atttaaagag aataaaataa aaaaacttaa tgaaaaagct taatgaaaaa
     4801 gtctaataaa aagtttaata aaaagcttaa tgaaaagctt aataaaaagt cttgttttcc
     4861 tgatgaaaac aagatttttt attaagcttt ttattatttc acttcgattt cacaagagag
     4921 acactctgat ttcaccaaat agaaataaaa taggttaaat aaattaaaaa atagaagcgt
     4981 aattaaataa ggttacaagg gggagtactt atgaaatggt atacatggtt gactattata
     5041 ttgataatat tgctttctat tccgcttaaa attaaattta taaaatggtg ggatagacga
     5101 caaaaaaatg ataaaggagg ttatgataga tgataaaagt aggagagtta aaattttctt
     5161 atggaaaaga taaacagata ttgcatggac ttaattttga agtaaaagaa ggagaaatct
     5221 ttggattcct tggacctaat ggttcaggaa aatccacaac acagaaaatc ttaaatggtg
     5281 ttttaaaagg ttacaatggt cacgtatccc tttttggtaa ggaagttaaa gcatacacag
     5341 aatctttata tcaaaagatt ggtgttttat ttgaatttcc ttatttatat accaatttaa
     5401 gtgcaattga taatttagaa tatttttcgt ctttttatcc taaaaatcaa aggagagaca
     5461 tatatgaact cttagatttg ttagagttta agaaagagtt tataaataag cctgtatcat
     5521 cctactcaaa aggtatgaag caacgtatta gcatggcaag agcattggta agtaatccaa
     5581 agttgttatt tttagatgag cctactagtg gacttgaccc atctggagct gtactatttc
     5641 gtaagattat tgaagaagaa cgaaaaaaag gaacaactat ctttttgact actcataata
     5701 tgctagatgc agatttaatg tgtaacaatg tggcttttat tgcagatgga aagattatgg
     5761 taatagataa acccaaaaat ttaaaaatga aaaatagcaa taataaagtt gaagtggaat
     5821 ttgtatataa tggaaatcga gatattgaaa ttgtagatat acaagagtta gaatctggaa
     5881 taacttttaa atatgatgaa atcttaagtg tacattcaaa agaacctaca ctagaagaag
     5941 tttttattaa atacacagga aggatgttag tgtaatgtat tctaaatttt ttaccttagt
     6001 aaaaaaagat ttttggatga tgatttatgg taaatttttt gtgctgacac ttggctctct
     6061 catactttat tcgtgttata ttaattttgg ttatgtaaag tttatggatg aagatagtta
     6121 tcacaataat gtatatctat ataatccaga gaatataagc atgagtgcat ctccattaat
     6181 taatcaagtt tcttctatag gtgaattaaa agcaaaactt gatgaagata caagtgcagt
     6241 ttgtattgat atgagttcca gtaagccgaa agtaatttta catgaaagta ttaaaggagt
     6301 tgacaattat agagctgact atgcattgtc attattatca tcagaaaaag accataatag
     6361 caaaatatca gacaacaatg caataattca aattaatgat aaggatacat ctgagataaa
     6421 acaaagaaaa gacatgactt gtgagatttt attctttgaa attgtagcta ttggattttt
     6481 agggattgcc tctttattat ttaaagaaaa gcagatggga gtaatgcgta ttactggtat
     6541 tttacctgta aaaagaagtc tttttatatt atcaaagata tttgcttttt ttatatgtga
     6601 cctatgtttt acagttcttt tagtagtatt taatattgga tttttagaag gaataaaaat
     6661 acttccacaa gtattagttc aagctagtat tttatcaatt attatgtctt tgattggaat
     6721 aggatgctca atgttattga gagattttaa gcaattttct cttatatatc ttgtggttgc
     6781 tgtattcata actacaccta tatttctatc tgcaaataca tcttttgaaa tagattggat
     6841 taaatatcat cccttctatc atgtttatag tcagcttaaa aatgcgtttt tcatgaattt
     6901 ttcaaaagaa ttagtttatt ttgtagtgtg ttctttgaca ataatagttc tatttttact
     6961 tgtaaaatat atttttaata aagagttggt tagggaggga taatcttgat aggattacat
     7021 taccagttaa agagtataaa aaaagataaa atgtgtataa tttccttctt tttaccagtt
     7081 gttatggcga ttttaataaa ttttatggga acaattgata tatcttcatt aggagagata
     7141 cgttttggta tagtgaagga tacacttcct gtacaaacag agcagtggtt gaagaagtat
     7201 ggtatggtaa gtgtatatga cacaaaaatg catttaatac gtgaaattaa ggatattaaa
     7261 acggacacaa ttggagttgt tagagatggt aaagggataa aaactgtgct ttatggaaat
     7321 gagattaatg ctataaaaaa tgttggaaaa acattgccag agatgtttag actaaagggt
     7381 gatttaaaag atgttgaggt tacaatattg cctaaagata gtattttaaa agagtttcaa
     7441 aatgttttta ttgctatgat tttaattact gctatgttta tgggatgcac ctttaatgca
     7501 atgaatatta tatcggaaaa agaagatgga atatcattta taaatgaagt tttaccacag
     7561 agtagaagtc agtatattat acaaaaagta tttttgggtc ttttatgtgg gtgtttatct
     7621 tcaatgataa ctgtatttat atgcttggat atttcaatat tagatatgat aatgatgttg
     7681 ttactcgttg tattttcagc atttatttca tcacttatag gtttatttat tggaaagttg
     7741 tcagaaggtc ttatggtagg aattgtttat attaaaatag taatgatttt atttataggt
     7801 actcctttgt taatatatct tctaggggtt aatacaagag gtatagggaa tttatgctat
     7861 ataatgccat cagttgctac atttaatggt attatggaga ttattaatgg taatgtcatt
     7921 attataaaag atattgtaat tttggctgta cattgtgtag tatggtttat gatatatatt
     7981 attttagata aaaaaagaaa gttatctttt agtagataac tcagtataat tataattggg
     8041 acaatttaat aggtgattta atatactttg tatagtgtaa tttatgtagt gcaatttgta
     8101 tactttaatt tatgtagtgt aatagttagt tttattttat aattattcaa tataatttag
     8161 taagaagttc agtacaatta gagctactta gtggaaaact taataggtac ttaaactata
     8221 taaatacttt attttcctcc atttgtacta taaatttaat ttatgatata aatggaggta
     8281 ttattatatg atttgataaa tttcatcatt ataacctaca agtaaaggta ttattatata
     8341 aagttaataa agatagtatc aaaatattat ttgctataaa gagaggggct caaaataaag
     8401 ataaacctat ttttcaatgt tgaaatgata aaatatattt gatttcatat aaaaaatttt
     8461 cttcataaat taaaaagtcc attttaagcc ccctcatttt taaccagata aatcgagtta
     8521 aactatttaa atatattact cggattcatt tgacttagat attccttgct tgtaaaattg
     8581 ttttgaatag gggatagcca aagcaaaaaa taaaagtata atccctgcta tgattattaa
     8641 attattaata gaaattacat ctgccattgg tccgaaaatc atcataccaa gaggaataaa
     8701 accagtatat ataataatag gtaagctaag aactcgacct agcatttgtg gtgctacatg
     8761 ctcttgcaac atagtcatag ctgatgcttg aataattgga attgaaaaat tcgtaaaaaa
     8821 cataaatata aaaaattgcc aagcttgtgt tacaagacct agaataattg aaaaaatagc
     8881 ataggataat attgctagaa aaaaggtctt atttctattt ttaaaaccac caaaaactcc
     8941 tataatcaat ccacctaaaa ccatacctaa aaatccaatc atttcaataa tagacaggta
     9001 tatataaccc ctgccaaatg ttcgttcaat aagaagtcct ataagaaatc ctgatggaac
     9061 acaaagaagc atgaatgctc cataagtgaa aagcactttt ctaatgaact tatctgaaaa
     9121 acagtacttt atccctgata ccatatctgc aaaaaatgag actttttcag tttgttttgg
     9181 tatattatgt ttaggaattt tgatgaatat caataataca atagcaataa ttgcagtaaa
     9241 tacatcaatt aaaagaactt gatttatttt attaccaaat ccaagtattg cagcagctac
     9301 aataggaact ataaattgta ctatagactg taaactacta tttataccat taaaacgcat
     9361 taaatgttct tctggtacaa gttgaggaat cattgcgttt acagcaggtg tttgtacacc
     9421 acttcctagt gaacgtataa cagatattac tgatatggct attaattcac ttgttgtgcc
     9481 atttgatgac atcataaata gaattagaac taatgtagat atagcaattg ttgaatctga
     9541 tacaattatg agtacctttc gattgtagta gtctgcccat acaccagcaa aaaaagaaat
     9601 tatcatttga ggtataaagg agcatacagt taaaactgtt acccatacac ctgatgatgt
     9661 ctgaagtgtg acataccata tcattgccat ttgtacaagt gatgagccaa ataaggaaat
     9721 atattgactt gctaaaaaaa gaatgacttt tttcttccat gtagaattag tatttttatt
     9781 ttccatccta ttcctccttt gatttgtgtg ttgaattttt cttgctctta aggataacac
     9841 acaaatcaaa gtctggagtt agagtggagg gctaggaaaa ttgttttttc ataatttgaa
     9901 gatgagattt catggttttt gcattatgtt cagcttcttt caatgaagtt atcagtttgt
     9961 cacacactga gataatttcg tcatcatctt ttggagtgtc aaggacagtt ttaatattag
    10021 tattaggatt agaagataaa gcatttaaca ttcgtaaaat agctgaaaga gaatagtttg
    10081 cacaacgaag tgcgcttata attttaagtc gctttatatc ttcgtcagtg tatacgcgat
    10141 aaccattttg ctttcttttt acttttaaca agccattcat ttcccaattt cttaaagtat
    10201 ctattgtaac atttaagtgt tctgcagttt cttttctagt taaaaagagt ttatttgttt
    10261 ctggagtatt tccatttaaa atctgtgtaa caatagtaag tgcttcctcg gcattagatt
    10321 tttctatttc taattgttgc aagtattgat ttacaagact aatagcctta tcaaagtccc
    10381 tatttgctga tgttcttaca atctcaaggg catcttttct taatcctctt tgtaccactt
    10441 caattttcaa ggctgtacga gcaaatctaa attgttctat atgaaaatca ttaaagatac
    10501 ggtaaccatt ggtattacgc ttaggtttag gtattaagtt tagttcttca tataaacgta
    10561 ctgtattagg atgtacacca attaaatctg ctacctttga tgtcgtgtaa gtgtccatta
    10621 gtattcaact ccttttagat atatagtatt agtcataata acaccaaaca aaagtctggt
    10681 gttatagtgg agggtttggt tatctaagag taattagttt atctaagagt gattaatcta
    10741 ttatagaagt aagtatatat ttttcaataa actcccatct atcacgaggt ttaaacacta
    10801 ttttagatgg tatggctatt actaagtcat attgtggaat acaacaaata gcatttcctc
    10861 cagttcccat tgctgaatac cacaaaacac catctttttt acatagccac cacagataac
    10921 cgtattcatt actattcatt ttaaccgatt cttcaatcca cgatttagat ataatacgtt
    10981 tattattcca aataccatca tttaaataca gaaaaccaaa tcgcaacata tctctagtac
    11041 tcaatgtaag tccccagcca cctatagaat aaccctgtct atcttttgcc caacccttaa
    11101 gattttttcc aaagaaatct tcaaaaccaa aatctttcat atcataatct ggtataactt
    11161 tcataccaat aggtttaaat aagtactcat tagcaaattc acgagcagat tttccagtag
    11221 tttttgttat aatagctgaa agtaggtgcg ttccagctga agaatatttg aaagtaccaa
    11281 tttcgccttt ttgaccaagt tgattcaagg tatattttat ccaatctttt tgtttgcaaa
    11341 gtctttctaa agactcagtc atatttttaa aagggtatgg tgcagtcatt gtaagtaggt
    11401 gacgtatagt tactgctttt ttacatattt cagaaggact acaagtatat tcagggaaaa
    11461 aatcaacaac cttttggtca acatctttta tatatccttt atctattgca atacctatta
    11521 aggctgatat aatacttttt gtaactgatg caacatgaaa agtatcatca gcattatatc
    11581 cattatagta cttttcaaag acaacttttc cttttcttgc aataattatt ccatttatat
    11641 tactatattg tgagattatt aaaggttcca gttgtgagag cttttctgaa ctcatgccaa
    11701 cacttgaagg gtctactttt tcaaatatta aatctaagca attgttttta ttcattaaag
    11761 atacctccct gttatagatt ttggattttc ttttagagtt tttggagaac cttctgcaat
    11821 tatataacct cctttgtcac caccttctgg accaagctca acaatccagt cacaaacatt
    11881 taatacatct atatcatgtt caacaacaat tacagaattt ccattttcca ccaattcatc
    11941 taataattga atcaacttag aagtatcata tagacttaga ccagtagttg gctcatccaa
    12001 tacataaagg atattaccat ttcgtttttt accaatttct tttgcaagct tgattctctg
    12061 ggattcacca ccacttaaag tcggtgtagg ttggcctaat tttatatacc ccatacctat
    12121 ttgttctaat acatttaagt gtgaaacaat atttggaata tccttgaaga aatgtacaga
    12181 ttcagaaaca ctcatattta aaatatcatg tatattttta tttttatatt taatagataa
    12241 agattcatca ttaaatcttt ttccctgaca ttcactgcat atatggtcta tacttagatt
    12301 atttcctaac cagacctttt catatccact accactacat ttaggacaag cacctttgga
    12361 gttaaatgag aaatgaccag ctgtaaatcc tcgttctaac gattctggct gacctgcaaa
    12421 tagtgtacgt atcttatccc atataccaat ataagaagca ggagttgagt tcatattttt
    12481 gccaatagga gattgagata tctctgaata tccagaaata tgtttagtac caatcagtct
    12541 atctgcaata gtttcaacaa ctgcataatc gtcttcctca cctatttcac tctctgaaga
    12601 atttgtttga ttattgatag tatggttatt aaattggctt ctaagaagag gaagtagggt
    12661 gtctgatata agtgaactct tgccactacc tgattttcca gatattccaa ccatagcacc
    12721 caaaggtaat gatacagtta catttttaag attatttgta tttgcatttt gaatagttat
    12781 acaaggtatt ttatcagtat gtgtaatatt tttattgctt gttctattag gcattggata
    12841 ttttccagat agatactgcc cagtaagtga tttgtcggat tgaagtagac cttctaaatc
    12901 tccttgatat actaattgac caccttcaac accagctttt ggaccaatat caataatatg
    12961 tcctgccatt tcaattgtgt ttttatcatg ctcaactact atgaccgtat tgcctaaatc
    13021 tttaagtttc tttattgatt ttaagatgtc tattttttct gattcatgca atccagctgt
    13081 tggttcatca agcacataaa ttaatgagtc catctctgaa tcgagatgtg aatttaaaaa
    13141 tagtctttgc atttccccac cactcaaagt tgacatttct cggtataagg atagatgacc
    13201 taatcgtgat tctattaaat gttgagtttt ggataaaata tcatttacaa gatttgtacc
    13261 aaatgtgttt aaattacctt gatttattaa gattttaagg aaatcatcaa tttctaaaat
    13321 cgtcattttt ccaagttctc caatatgttt tccgtttaaa agtactctac gtgcttcttc
    13381 accaatttta aaaccattgc aatctgaaca ggcagtcata gtataaactc catttaaatc
    13441 ttcgcccctt ttgtaacgat tgtgaaatac ctttgataaa gaggaacttt ttttaccatt
    13501 ttcataatga ccatagagta cttcatttct tatttcttca ggtagttgta agaattgtat
    13561 gtctaaataa tcgttgaatt ttttagaaaa tactctcaaa tatcctggtg taatctttgc
    13621 tctatctaag acttgtttta gggtgtcatg cttatcaagg actaattttt caatattaat
    13681 ctcaaaatat gcaccatgtc cagagcactt catacacatt ccatctgaag agttgtatga
    13741 gaaataactt ggtcttaatc gttcttcttc atacccacaa ttattgcaaa ttagattatc
    13801 gcttacagga gtatcacaca tagtacacat tatttgacct tccactgaat acaatagtgt
    13861 aagcatgttg agaatattgg ttcttgtgcc tactgttgaa cgaggattgc tctggcgaat
    13921 aatattttgt tgaacagcta tagtgggagc taaaccttga atattatcaa acttatcctc
    13981 actatcaata cctgcaagta tacctaaaga ttgcagatat tgtttacgcc cttcctcaaa
    14041 tacaatgtca aacataaggc tagacttacc tgagccactg acacctgttg caacaacaag
    14101 cttatctttg gggatagaaa catctatatt tttaagattg tgtatctttg ctccattaat
    14161 ttttatttct ttcattggtt cacctcattt tatcaatttt atcaaaccgt aaaataggga
    14221 tatattaatc cttaatacaa gaatagatta ggtgaggtta ttaaaaaggt tctataggta
    14281 aatatatatc gaccaagtgt ctattttgag ggtgattgtg tgggttgtta agatatactt
    14341 caaaagggaa ggaggcacgt agcttataat taccatttga tagccattca ttatacataa
    14401 aatcccacgc atcactatat tcagattttg aaatatcaaa atgtcccaca agatattttc
    14461 cagaaggtat tatcatattg ccaagattgt cattctcttt gacaacaatg ttatctggaa
    14521 tggtcatgca aatactagtc cttaattgat gctcctgtgt aaattgtgga ttatcatgat
    14581 aaatggctaa tatttgtgtg ttttcaagtc caagtagatt ctgctcactt gcatatgtga
    14641 ataaccgttc taaaagtttt gggaaagtac tggttagatt accatatgaa ccagtatacc
    14701 tagtgtaaat agtattcata ttatcaagtt ctagaatttc aatttcacct ttaacctctc
    14761 tagtattctt acatttaggt tttgatatgc ttctattgta ctgagaaatt ttatatggtt
    14821 ctttgcaatt cttgctataa ttgtttctat attttatagg gcttatttta tagtactttt
    14881 taaatgctct cgagaaaact gctgaatcag taaagccgaa gtagtgtgca atatcagtaa
    14941 cagtcatgtc agttcgatga ataagaaaag cagttgcatt ttctagtttt agcctattta
    15001 catattgtgc caaagtttct ttttctattg ctttgaagat tctatgaaaa tgaaattttg
    15061 aaaaacctgc tatattagaa agttcatcaa gagaaagagg ttcatgtaaa tgttcttcta
    15121 tatagtcttg cactttgcaa atacgattta gatattcttt tttactttta gcatttgtca
    15181 tatatagccc tccttattgt gtaaaatgtg tatcaattaa tttatagcaa tatagt
//
LOCUS       DAEMHZ010000102        15059 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000102 DAEMHZ010000000
VERSION     DAEMHZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.102"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..508
                     /locus_tag="KPK72_002767"
     CDS             <1..508
                     /locus_tag="KPK72_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489965.1"
                     /translation="VNKIVFKQLNISNTLKAPKNLRDILKVNVNSKIFDVKPMKNLIN
                     KGSNLKLCNLIVFGCIEVEIDYSCKNRSNFKTVNKSNDIKKDKYYKNDIQNNNENIND
                     CKKQISFGDNKVKKITKTFGFSCFICSPVGIIYEDMKNINIKIEHTSINELNPEELFV
                     NTSLLLYY"
     gene            798..1340
                     /locus_tag="KPK72_002768"
     CDS             798..1340
                     /locus_tag="KPK72_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2489966.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1668..3161
                     /locus_tag="KPK72_002769"
     CDS             1668..3161
                     /locus_tag="KPK72_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2489967.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEQSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            3235..3468
                     /locus_tag="KPK72_002770"
     CDS             3235..3468
                     /locus_tag="KPK72_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489968.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLIPPTRHVILHLNACCKWKEYFRTKKKFYRS"
     gene            3624..4388
                     /locus_tag="KPK72_002771"
     CDS             3624..4388
                     /locus_tag="KPK72_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2489969.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETIQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4673..7852)
                     /locus_tag="KPK72_002772"
     CDS             complement(4673..7852)
                     /locus_tag="KPK72_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2489970.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVIKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            8132..10309
                     /locus_tag="KPK72_002773"
     CDS             8132..10309
                     /locus_tag="KPK72_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2489971.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            10323..10745
                     /locus_tag="KPK72_002774"
     CDS             10323..10745
                     /locus_tag="KPK72_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2489972.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10919..11494
                     /locus_tag="KPK72_002775"
     CDS             10919..11494
                     /locus_tag="KPK72_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2489973.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKALKKAYKLGMKIAIDING"
     gene            complement(11619..12335)
                     /locus_tag="KPK72_002776"
     CDS             complement(11619..12335)
                     /locus_tag="KPK72_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2489974.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            12500..13225
                     /locus_tag="KPK72_002777"
     CDS             12500..13225
                     /locus_tag="KPK72_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2489975.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVDIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            13285..14877
                     /gene="hcp"
                     /locus_tag="KPK72_002778"
                     /gene_synonym="priS"
     CDS             13285..14877
                     /gene="hcp"
                     /locus_tag="KPK72_002778"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG2489976.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
BASE COUNT         5857 a         1651 c         2385 g         5166 t
ORIGIN      
        1 tgtaaataaa attgtattca aacaattgaa tataagtaat actttaaaag ctcctaaaaa
       61 tctaagagat atattaaaag taaatgtaaa tagtaaaata tttgatgtta aacctatgaa
      121 aaatcttata aacaaaggta gtaatttgaa attatgtaac ttgatagttt ttgggtgtat
      181 agaagttgaa atagactata gttgtaaaaa taggagtaat tttaaaacag taaataagtc
      241 taatgacatt aaaaaagata agtattataa aaatgatata caaaataaca atgaaaatat
      301 aaacgattgt aaaaagcaaa taagttttgg tgataataag gtaaaaaaaa taacaaaaac
      361 ttttggattt tcttgtttca tttgttctcc agttggaatt atttatgaag atatgaaaaa
      421 tataaatata aaaattgaac atacatcaat aaatgaatta aatccagaag aattatttgt
      481 aaatacatca cttttattgt attattaagt attttaaagc taaaacttaa acttattaaa
      541 atagcatttg gttaaaggaa aatagatatt gatatattta tcatatatat aaatgattaa
      601 ttaaacaatt ataaataata agtatattat aatatttaaa aaatatagat ttatgataaa
      661 ataagacaaa tatgttaaaa aaatgataat cacggagaac ttttaccttc ttcaattaat
      721 atattattat tagtaataac tttaaaatta tattactatt atataaaaat tggcttaaaa
      781 gaaggtgaaa aaagtaaatg aaaatatgca taacagtagg tcatagtata ctaaaaggtg
      841 gtaaaagtac aggtgtaaat ggtatagttg atgaatacaa ttataataaa aaattagctc
      901 ctatgttagc tgagatgctt ataagtcaag gtaatacagt agatgtaata atttgtccag
      961 aaaggcactt tatgtcagaa agagaagaat ttttctatag agttccaaaa gtaaacagtg
     1021 gtaaatacga tttattagta gagctacatt taaataaagc tgatggaaca caatgtggta
     1081 cagaagtgct ttactatggc actgaaggat tagaatatgc taaaagagta tctaatagac
     1141 ttggagaatt atttgaaaat agaggagcta aaaaaagaga aaatttatat atattgagaa
     1201 atacaaatcc agttgcaata cagattgaaa gttttttctg tgataatgtg aatgattgta
     1261 acaaagcaaa tgaatcaggt tatgattata tagcacgatt gattacagaa ggtatattga
     1321 ataaagatat aaatatgtga gattatggtg atttaggtat tgattaatac cttttaatat
     1381 tagatataaa taaaaataca atgtattagt ataatttaaa ctttcaaaag atagattagt
     1441 ttgattattg acaagagata cctccttgaa aatattgatt tatcagtata tttaaggagg
     1501 tatttaaata taaaaattta attaatttgc tactaaacta taggtttata gagtatatat
     1561 ttttatcttc agtctaataa ataatataaa aatagtagaa ccttgtatta taataatata
     1621 atgtagaaaa atatatagat aagataaata taataaggtg gtaatatatg aaatatacat
     1681 ttgaaaaatt taagttaaat gaaaagatac ttaagtcatt gaagagttta ggatataata
     1741 taccttctag agtacaaaga gaagttattc caaaattatt aaaaggtcaa aacttagtag
     1801 ttaggtcaaa aacaggaagt ggaaagacag caagttttgc aataccttta tgcgagaata
     1861 taaatgtaga ttacaataat attcaagctc ttatagtggt tccaacaaga gaattagcct
     1921 tgcaagtaaa agatgagata tcagatatag ggagactaaa gaaggtgaga tgtagtgcta
     1981 tatttggcaa acaatctata aaagaccaaa tagctgaatt gaaacaaagg gttcatattg
     2041 ttgtagctac tccaggcaga atacttgacc atataaatag aggttctatt aagttggaaa
     2101 atgtaaagta tttagtaata gatgaggcag ataaaatgtt taataaaggt tttgttgaac
     2161 aaatggaaaa aatattatta aatttaccta aggagaaaat agtaagtctt ttttcagcta
     2221 caatagatga agagataaaa tatatatgtg aaaaatatat gctagattac agtgttataa
     2281 atatagaaga aaatgagagc gatactaatc aaaaaacaag acaaatagat gacaaaataa
     2341 tcaaggcaaa tggaagagag aagtacatcc ttttaaaaga attaatatac agcgaaaatc
     2401 caaagagtgt gataatattt tgtaatacca aagagaaagt atctaaatta tataacaaga
     2461 tgagtaaaga gggtttttta ataagagagt tacatgcaga tttatcacaa gaaagaagaa
     2521 tttttgtaat taaggatttt aaaaaccaaa aatttaatat attagttagt agtgatgtag
     2581 cgtccagagg aatacatatt gatgatattt ctttagtaat aaattatgat gtgccacaag
     2641 ataaagaaaa ctatatacat agaataggta gaactgggag aaaaggaaat agtgggaagg
     2701 caattactat agtaactgaa aaggatgaaa aatatataga gaatatagaa acgtatatag
     2761 gatataaaat aaatgaatta aaagatatag aacaaagtag aattacacat ggcaaagttt
     2821 tgtttgaaga atattcaaaa ggaatcctaa aaaaagtatc aaagagaaaa aaagttgata
     2881 aaaattctta caaaagtaaa gatataaaac taaatataga aagtgaagtt gttaaattat
     2941 atttaaatgc tggtaagaag aagaaaataa gagttttaga tatagttggt gcatttagta
     3001 atataaaagg tataactaat gatgatatag gtgtaattga agttcaagat ttatgttcat
     3061 atgtagatat attaaattat aagggagatt tgatattaaa aaaatataag gagataccta
     3121 taaagaaaaa aatggttaaa gtgaaaagag atatacgata atttagtcaa tattgatgaa
     3181 ttgctttgtt atcgaaatga agttgcaaaa aacacaatag aagttgttaa ggatttgaaa
     3241 gctgaagata tgaaacgaaa ggtctctcca attgacttag aaaggatttt ggtgactgga
     3301 ggagtaataa agcatgaaga ttctatttgg ttgttggact tttgggggaa taaagatatt
     3361 gctggaattt tgcttatacc tcctactcgt catgttatac ttcatttgaa tgcttgttgt
     3421 aaatggaagg aatattttcg tacaaaaaag aaattttaca gaagttaaat tataataaat
     3481 tagacaaaca atagataaat tagcaaggaa tgttgatttt atctacattc taagttatac
     3541 tatttttaag tggctaaggg aatagtcatt ttaaatataa gtaataaata tatacctata
     3601 gggatattaa aggggggaaa tatatggcat attttaacta taacgacaaa cagtgctact
     3661 ataatgaaat tggggatggt acaccattac tttttttaca tggaaataca gcctcttcca
     3721 agatgtttaa tgatataatc gatttttata aagatgaata taaagtaata ttaattgatt
     3781 ttgtaggtca tggaaaatct caaatggtcg ataaattttc tgctgactta tggtttgatg
     3841 aggctatgca agtgatatgt tttttagaag caatgaacta taaaaaagta aacataattg
     3901 gtagtagtgg aggagctctt gttgcattaa atgttgcact tgagagacct gatttagtga
     3961 ataaggtaat tgcagatagt tttgaaggag aagttcctct agagtcattt gtacaaaatg
     4021 tgaaaataga gcgtgaagca tctaagcaag atgatggtgc aaaagctttt tatatttata
     4081 atcaaggaga aaactgggaa aaagttgtag ataatgatac agaagctatt tttgaacatt
     4141 ataaaactat aggaaaattt tttcataagc ctctagaaac tatacaacca gaggtgcttt
     4201 taacaggaag tagagaagat gagtttgtat ctctaatatc tagtgatttt tttgaaaata
     4261 ctttttcatt acttcttgaa aagattaaaa atggtaaaat gtatttgttt gataaaggtg
     4321 gtcatcctgc aattttatcg aatggattag atttttcaaa tgtagcaaag aaatttttag
     4381 aggaataatt taaccaaaat ttacaataat gaaaactgta aaatagattg caaaacttac
     4441 aaaagtgagt gtacatatac ttcattatta tgatgaaata ggattgttaa aacaaaatat
     4501 tctaatagtc aagttattac atttgtatat gcaatatgta tagaaagaga tatcattaga
     4561 ttgtaaagtt gggattaaat aacaatatta aattttattt attcagatat tttagcttaa
     4621 ctatataaat gttagaacaa atttatatag ttaagctgta ggattaaata atttagttaa
     4681 ataagtttat tatctcctca tcagatagac tttttaatac accttcatta gaaagttctc
     4741 catttataaa ttgatttata agctcttttt tactttcttg taattttatt attttttctt
     4801 ctatagtccc tttagcaatc aattttataa cttgtactga atttttttga ccaaatctat
     4861 gagctctatc actagcttga ttttctactg atggattcca ccatgggtca aagtgaatta
     4921 ccatgtcagc agatgtaaga tttaaaccag ttccgcctgc ttttaaagat attaaaaaaa
     4981 cttttttatc catactatta ttgaactcat ctacaagttc aagtctttct ttggcattag
     5041 ttttaccatc tatataatgg tatttaattt tatacttatc aagttcctta cttatgttct
     5101 taagcacaga agtaaattgt gaaaacaata atattttgtg gttttcgttt atactatccc
     5161 taagtatttc taaacatgtt tcaatttttg aacttttttt attgtaatct tttattacta
     5221 tacttgggtc taaacaaagt tgtcttagtt tagttaaata agaaaatata actattttat
     5281 ctttctttaa atttttatct ttcattttat cttgtatatc ttttgaatat acactatata
     5341 tctttttctg ctctttattc aattcaacaa aaaagttttt ttcaatctta tctggaagtt
     5401 ctttcataac ttgtttttta cttcttctaa gaataaaagg ttttatcaat tttttcaggt
     5461 ttttaacatt atcttcttta tttataaaca attcctgaaa ctttgcttta ctatatagat
     5521 agccaggcat tataaaatca aatatagacc ataattctag tagattattt tccataggtg
     5581 taccagtgag tgcaaattta ttttctgcat ttatactttt tacagactca gttactaaag
     5641 ctactggatt ttttatattt tgagcttcat ctaatataca ataatcaaac tttatttcac
     5701 tatatttttc taaatcattt ctaagagtac catatgttgt aagtattaca tcaaaatttt
     5761 ctaattccat aaaacatttt tctctatctc ttttatttcc atgtatcaat aaaactttta
     5821 tatctggagc aaacttttca aattcatttt tccaattata aatgagtgat gtcggtgtaa
     5881 caataagact ttttttatta gataatgata ataaaaatgc aatagtctgt attgttttac
     5941 caagacccat ttcatcagct aatataccac caaatttata ataatctaaa actttaaacc
     6001 aattgaggcc atttatttga tagtcacgaa ggtttgcatt taaatttgtg ggaatttcta
     6061 aatttatgct atcaaaattt ctaaattttt cacatatact gtctagttct tttttaccat
     6121 ctataaactt taaattttta ctacgtatta catcatttat atatagagct ttatttttat
     6181 gaattttatt attttttgat ttatccatta tatctagatt ttcaactaat tcaaaaaaat
     6241 cttttgtttc tctttctgat aaatctataa agtttccatc tttaagttta taaaaagtac
     6301 gtttttcttg aaaagctttc aaaatatcct tgtactcttt tggatttata tcagatatat
     6361 taaaattaaa ttctaggtaa ttctggttag tctgattgat tgaagcttga attgatgaag
     6421 cgttatatat ttttttcaat ttaaacttct cagaataata tatatctcca aattccttta
     6481 gagattctaa ttgtaaatct aaaaatgtaa ataggtcatg gtcagttcct ttaaacacat
     6541 aatgattatt ttcctttgta aattgtgaag aagtaagttt attttcaata gtagcttcct
     6601 tttgtatgtt ggggagcaca aattttttat cttcatcatc atatatatac tttacatcac
     6661 aacatatgtt gccatcaatc atatcaaagt agaactcaga tttaaaatct tttgttatat
     6721 tattttttat gttatcatct aaatgaactt cttgagatat agattttaat ctaggaacta
     6781 gcaatcctaa aacatcctta gaatcctctt tatcaaattt taactcttta gtctttgata
     6841 atatttgata tatttttgaa taatcatgag cttttttatg tgataataaa tagatattgc
     6901 catcatataa aagtacatca ttttttttag taagtggaac aggtagtgaa tctttagaat
     6961 ataagactat tttatcttct atatttttaa aatcaaacct tacaggtaga gtatcattta
     7021 atatctttgg atgatattcc tctttagaaa aaataagggt aaaatccttg tacttaatag
     7081 catttaaaaa ttgttttaaa gagcttgcat ttatctccat aaattttttt gaatgtgatg
     7141 aagaaaagtt gtatgaaaga tttgtcccat attcttctat aatatctact aacttctcat
     7201 cttcatcagc aaaataatga atgttggggt tgtaagtaaa tttcttccca aaatctaatt
     7261 catatccatt aagttttgaa tatgcaaatt cagtaatatt ttttaaaatg tacattttat
     7321 caattcctat tttaaattca gcaattgctt tatttgaatc cacatttatc aatacatcta
     7381 aattaaccct ttctctttta gtattgtcta acaaagaaag taattgcttg cttaaatcta
     7441 agctggtttt agaatttgta ttagaaagct tatccttatc tgcacctaat atatctatta
     7501 gaataggagg aacatctttt ttatttttta tttgttttaa gctttttatc acaatagcaa
     7561 ctatatgctt acatataaaa atacttttgc cctttgattg acagtcagcg caactacaat
     7621 cattttcaat tatcttaaag tgctttatat ctatagttat tgaattaaaa taaaagctat
     7681 aattatcttc agaattgact ccaccacgaa atgtcaacag gttattttgt aggtcatcaa
     7741 tatctatgtc tatataatca actaattgat tagtataata ttttacacct cttgaccatc
     7801 ttgatggttc tgtatttctt cttattagtt tatatatcat atctattttc atattattca
     7861 ccttacctca ctgtatatat tataatctaa atgcattcaa tttacactct aaatttaaga
     7921 ctaaaattaa ggcagatatg aatagctttt atctatcaca atatatgtgt aaataacaat
     7981 ttactattag atttttattg aattatctta taactatgaa tgaaaaataa ttatataaat
     8041 agattattaa taaatataaa atcttattat taaaaatatt cttagaaatt acactatata
     8101 aaatataaag tatgatattt gaataaaaag aatgtataat aagtataata atttgtattt
     8161 attattggga aaggaaaatg ttaatatgga tagttataga caacttaaag ctaaaatacc
     8221 ctgtaaggaa acaggtatag aaataaagag gactttatgt gatatttgtt ctccaggagt
     8281 acattgtgga attgatgcat atgttaaaga tggtaaagta ataaaaatag aaggtacaaa
     8341 agaacatcca atgaataaag ggttgttatg taccaaaggt ctttgcaata gagaatatat
     8401 ctatagagaa gatagaataa aaacacctct aaaaagaatt ggaaaaagag gtgaaggaaa
     8461 atttaaacca atatcatggg atgaagctta tagagaaata gcagaaaaat taaatagagt
     8521 aaaatctgag tatggagcaa agcatgtagc tttttttacg ggttatggaa agtggtatag
     8581 acctttttta caaaggtttg catcttcatt tggttcacct aattatggta cagaatctag
     8641 ttcatgttca acatcagcac agatggctgg aaatgttgct actggaagaa actttacaat
     8701 gcaagatatg gagaatgcaa aaacttattt agggtgggga tttaatccat actactctaa
     8761 atttttaaat agtgtgaatc taataaaatt aaaagaaaaa ggtctaaaat ttataattgt
     8821 agaccctaga ataacaccga caactcaaaa actagcagat ttacatttaa gaattaaacc
     8881 tggaactgat ggagctttag ctcttggaat ggcgaatcta tttatacaaa agaatttaat
     8941 agatagagaa tacatagaaa agtatgtaca tggatttaat gaatataaag aatatgtaaa
     9001 attatttgat ttagatactg taagtagaat tacaggagtt tctaaagata atatagtaaa
     9061 agctgttgag atgttgtcac aaggtccatt ttgtataaat gaaagtgcag ctccaatagt
     9121 tcaccatcaa aatggatttc aaaattatag agcaatgatg tctttatctg ctataacagg
     9181 taattatgat agaataggtg gaaacatacc aaaacaagat tactttgctg gtaagatagg
     9241 tggattccaa acactagaaa aagagtttgt aaaagaagtt ctacctaaaa atataaatga
     9301 aagaattggt ggagaaagat ttcctctatg gaatgaaatg gtgactcaat ttcaagctat
     9361 ggatttacct aggcaaatag aagaaaagac accatatgaa ataagagctt tgttttgtca
     9421 tgggataaat gctagaatgt ttcctaattc agggagactt tttaaagcgt tgaatagtgt
     9481 agattttttt gtgaatatag atttatttat gacagataca tcaaaatatg cagatatact
     9541 tttacctgca tgtacatcat ttgagagaga agaatgtaag agttatccag gagggtatat
     9601 tacatatacc aaaaaagtta ttgataaatt atacgattca aaatcagatg tagaaatact
     9661 ttcagattta gcaaatgcta tgaatataga tgatgattta ttaaaagcag gttatgaagc
     9721 aagtgtaaga catatgttta aaaatacatg tgtagatgta gataaactta aagaaagtga
     9781 tttaccatta aaatgtaaag attttaagcc atatatagta ggttcatata caagagaagg
     9841 gtatgatact agcacttcta ggtttgaatt aaaatctaca attattgaaa aatataaaga
     9901 ttttgggctg gatgcactac caacatatag aggatttaat gataatatag atgatgaata
     9961 tactcattta ttgacatcag gagcaagaat ttcaaatgca ctacattcaa gacttcatga
    10021 catcaaactt tcaagaatat taaaaaatga agctagttgt gatatttcta gcttagatgc
    10081 aaaagaattg ggtgttatca gtggagatta tataaaaata actactaaga taggaagttt
    10141 ggatgtaaaa gtcaatgtta ctgatagagt cttatataag aatatacata tgtatcatgg
    10201 atatagagaa gctgatataa atagtattat ttgtgataat tttgaccctt atagtggatt
    10261 ccctgcgtat agaagttgta agtgtaagat tgaaaaaatg aatagataaa tggagagata
    10321 taatgaaaag tttatttaaa tttaataaag aattatgtac agcatgtgga gcatgtttgc
    10381 ttgcatgtat agaccaaaat gataataata tagacaatgg tgaacttttt agacatgtat
    10441 ttacaataga ggaaggtgaa aatatagaat atttttcatt agcatgtata cattgtaatg
    10501 agccaaaatg tttaggtgta tgtcctaaaa attgttttaa aaaagaagaa ggattcattg
    10561 tattagacaa tcaaaactgt atagggtgta aactttgtga aaaagcttgt gaatatggag
    10621 ccttaattta tggaaatgat ggtaaggcaa ataagtgtaa tggttgtatt gagagatata
    10681 agtgtggtat ggattcacct tgtgtaatgg tatgccctac aggagcatta aaatttaata
    10741 aataattgta ttaaattgct atactacttt taaataattg tattaagaat tattttttgc
    10801 tatacttttg taatataaaa tataaataaa acaaatacaa agtatataaa atatattttt
    10861 agaataaagt gtgctttaga ttaaatttag aaaaataaat tagacaggag aacttaatat
    10921 ggtaaaagta ttagccttta tgggcagtcc aagaaaaaaa gggaatgtag acacaatact
    10981 tgatgagata ataaaaggtg taaatgataa taatgttgaa gttaaaaaat attatctaaa
    11041 tgatatgaat attaaaggat gtcaaggctg tctatactgt agaaaagttc ctacttgtgc
    11101 atttaaagac gatatgattg acatatatga agatataaaa agtgctgaat atataataat
    11161 tggttcgcca gtatatatat gtcaggtatc agctcaaaca aagctacttt tagatagatt
    11221 gtatccttta acagagataa ataagtctaa acatatacct aggtttggtc aaaaaaagtt
    11281 aatcatggtc tatactcaag cagctcctat ttcgttttta tttaaaaaat attttaagta
    11341 tactgcaaaa catttaaagg gcatgggatt aaatcactac aaaactatag ttgtaaccaa
    11401 agcatttgaa agaaattcca ctaaaaaaaa ttttaaggca ttgaaaaaag cctataaact
    11461 tggaatgaaa atagccattg atatcaatgg atagataaaa gtttaattta tagtatagct
    11521 aaaggagcat taattattaa tactcctttt ttgtataaag gtttctagaa aataactaat
    11581 aaaaaacaat attatttgaa tacagatatt atacaagcct agatatctaa cttgttttga
    11641 aaataaaaga tttcaaattt atttttgtta aaagataaca agccttcatc tcgcatcttg
    11701 ccaagctctc ttgacataga acttctgtca acacataaaa aatcagccat ttcatttctt
    11761 gaaaagggaa ttgtaaactg gtggctacct gtttgttcta tctgttttga aaaatacata
    11821 agcaatttct cgcgagttgt tttacgagaa agtaattcta ttttattgtg aagtaaaatg
    11881 tttttttcag ctattaattg tagcatattt tcaacaagcc tattatgaaa tacacatgca
    11941 gatgaacaaa taccagttac acttttaaac tttataaata aaacttcgca agaagatgtt
    12001 gtggttactg taacattact tttatgtagt ctagtacatg cgaatacctc tccaaataaa
    12061 tctccaggca agagttcagc tacaatattc ctattccctt cgatatcttc ctttattata
    12121 tgagcacttc ctttaagcat aattccaata gaagtaactt cacttccaac agagaatatt
    12181 atactatctt ttgggtaagt cttatctaca acacctaaac attccaacat ttcttcaagt
    12241 tcatcacttt taattccttc aaatagagga atttttttaa gtaggtctat atgcttttct
    12301 aaaaattttt tcaaataatc acttccttgt tgcatatgca accgataatt tgttttcatt
    12361 gtactatact aagtttataa aagcaagggg atataaaaat cctactacaa gaaatttgaa
    12421 ataaagaaat ataggtatta aaaataaata tataagaaat caatacttta aattaaaaaa
    12481 attttatgag gtgatataaa tgatgagaaa aataattaaa attgatgagg aaaagtgtaa
    12541 tggttgtgga ctatgtgttg aagcttgtca tgaagaagct ataggaatgg taaatggaaa
    12601 agcaaaactt ttaagagatg actattgtga tgggctggga gattgtctgc caacttgtcc
    12661 aacaaatgcg atttcatttg agtatagaga agcagcagaa tatgatgaag ctgcagtaaa
    12721 agcaaatatg gaagcaaaga aagcacaaaa gaaaacttta gcatgtggat gtccaggttc
    12781 tcagtctaag tcaataaata gagaggtttc aaatagtacg tctataagca atgatatagt
    12841 agatataaaa ggttctcaac ttaatcaatg gccagtacaa ataaaattag taccaactaa
    12901 tgcatcatat ttaaagaatg caagtttttt aatagcagca gactgtacag cgtatgcata
    12961 tggaaatttt cataataaat ttatgaaaaa taaggttaca ttaatcggat gccctaaact
    13021 tgatgaagtt gactatgctg aaaaacttac agagatttta aaagaaaatg atattaaaaa
    13081 tatagtagta acacgtatgg aagttccttg ctgtggtggt atagtaaatg ctgttaagac
    13141 agctctacaa aatagtggaa aaatgatacc atggcaaata gttactatat ctgtagatgg
    13201 aaaaattgta gatggagaaa tttaattaag tgaattatag aaaaatataa taaattttaa
    13261 taaataaaaa gggaggaata aaaaatggaa aaaatgtttt gttttcaatg tgaacaaaca
    13321 gcaggaggta aaggttgtac tataaatggt gtctgtggta aaaagggggg tacagctaac
    13381 ttacaagacg aacttacagg agtgttgatt ggtctttcaa gagcaactat tggaaataag
    13441 aatagaccaa caagtgaaac agataaaatt atgatagagg ggctattcac tactataacg
    13501 aatgtaagtt ttgatgatga agctataaaa agacaaattg aaaaaactga ggtagaaaaa
    13561 agtaagcttg tacctagatg ttcagattgt tcaacaactt gtaatagaaa tgataactat
    13621 gatatgaagg atttatggaa tgataatgag gacatacgtt cattaaaatc attaatctta
    13681 tttggattaa gaggtatggc tgcctatgca tatcatgcaa tggtacttgg ttatacagac
    13741 aaagacgtaa atgaattttt ctatgaagga ttaatagcag ttggaggaga cttgtctatt
    13801 gacgaacttt taggtctagt tatgaaaact ggagaaataa acttaaaatg tatggaattg
    13861 cttgatagag caaatactga aacttatgga acaccagaac cgactcaggt atctatgaaa
    13921 atcgagaaag ggccatttat tgtagttaca ggacatgacc tttatgattt acacaaatta
    13981 ttagaacaaa cgaaagataa gggtataaat atatatactc atggtgagtt attaccagca
    14041 catgcatatc caaaactaaa agaatataaa catctaaaag gtaattttgg tacagcatgg
    14101 caaaaccagc aaaaagaatt tgataatatt cctgctccaa tattatttac aacaaactgt
    14161 ttaatgcctg ttaaagatag ttatagagat agagtattta ctacagaagt agttgcttat
    14221 ccaggtatgg ttcatattga tgaagataaa gatttttcta gtgtaataga aaaagctctt
    14281 gaacttggag gatatagaga agatgttcat atgactggta ttaatggagg agatacatta
    14341 actactggtt ttgcaagagg tacagtttca tctgtacaag ataaagtagt agatgctgta
    14401 aagagtggag ctataagaca ctttttattg gttggaggtt gtgatggtgc aaaacctgga
    14461 agaaattatt acactgaatt tgtaaaacaa gctcctaaag acactgttat attgacactt
    14521 gcatgtggaa aatatagatt taatgattta gaccttgggg atataggtgg cttacctcgt
    14581 ttaatggata tgggacaatg taatgatgct tatagtgcaa ttaaagtggc agtttcattg
    14641 gcaaatgctt ttgaatgtgg agtaaatgat ttgcctctta gcatggtact gtcatggtat
    14701 gagcagaaag ctgtttgtat acttcttaca ctacttcatt taggtatcaa aaatatctat
    14761 ataggacctt ctttaccagc attcttatct aagaatgtat tagatatatt ggttgataaa
    14821 tttgcattga cacctatatc tactcctgaa gaagatatga agaagatttt aggataaata
    14881 aatatatcta tacttaaaga tatttatagt taaaatattt ttaagtatgc cacaattaag
    14941 ataaggggtt aaacttaaaa tttgattaaa aaatcaattt tgagatgacc ctttatttat
    15001 tttttgtaat aattgaaaaa taaacctgct ttcagtatat tcttttaaga atttttgta
//
LOCUS       DAEMHZ010000103        14821 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000103 DAEMHZ010000000
VERSION     DAEMHZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.103"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..289
                     /locus_tag="KPK72_002779"
     CDS             134..289
                     /locus_tag="KPK72_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2489977.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            419..1432
                     /gene="aroF"
                     /locus_tag="KPK72_002780"
     CDS             419..1432
                     /gene="aroF"
                     /locus_tag="KPK72_002780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2489978.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1454..2518
                     /locus_tag="KPK72_002781"
     CDS             1454..2518
                     /locus_tag="KPK72_002781"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG2489979.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2518..3831
                     /gene="aroA"
                     /locus_tag="KPK72_002782"
     CDS             2518..3831
                     /gene="aroA"
                     /locus_tag="KPK72_002782"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG2489980.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3809..4888
                     /gene="aroC"
                     /locus_tag="KPK72_002783"
     CDS             3809..4888
                     /gene="aroC"
                     /locus_tag="KPK72_002783"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG2489981.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4891..6087
                     /gene="pheA"
                     /locus_tag="KPK72_002784"
     CDS             4891..6087
                     /gene="pheA"
                     /locus_tag="KPK72_002784"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG2489982.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVHFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6134..6943
                     /gene="aroE"
                     /locus_tag="KPK72_002785"
     CDS             6134..6943
                     /gene="aroE"
                     /locus_tag="KPK72_002785"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2489983.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNIGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6955..7488
                     /locus_tag="KPK72_002786"
     CDS             6955..7488
                     /locus_tag="KPK72_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG2489984.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SKKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7509..8348
                     /locus_tag="KPK72_002787"
     CDS             7509..8348
                     /locus_tag="KPK72_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG2489985.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8616..10097
                     /locus_tag="KPK72_002788"
     CDS             8616..10097
                     /locus_tag="KPK72_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2489986.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10130..13567
                     /locus_tag="KPK72_002789"
     CDS             10130..13567
                     /locus_tag="KPK72_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2489987.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYQR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13585..14331
                     /locus_tag="KPK72_002790"
     CDS             13585..14331
                     /locus_tag="KPK72_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG2489988.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14280..14588)
                     /locus_tag="KPK72_002791"
     CDS             complement(14280..14588)
                     /locus_tag="KPK72_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2489989.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         6147 a         1453 c         2463 g         4758 t
ORIGIN      
        1 gagaagctga tttaagtaaa taaaaaaata ttttaaattg aaaatattta tagaaaatat
       61 tgacaattat attttctttg ttataattat taaacaagaa gaataataaa ttataaaaat
      121 ggaggataat actatgaaag gaatattaaa agaactaaca gcaaaacttt atagcttttt
      181 ttactttatg ttctggggct tttattttag cgctggctat ttcttttaca aaaatatatg
      241 tacttccttt ctggtgaggc cttattttag tgattttgag cataagtaaa ttttataaaa
      301 atgattattt tatatattta tataagattt agtgcttgat agattatttt agagaactca
      361 ttagtgagtt ctctttttta ttttaaatat aaaattataa tattgggagg tagtaaaaat
      421 gatagtagta ttaaaaatgg gagcagataa aaatgaagtt aaaaaattaa ttgaggctat
      481 tgggagagaa ggagtagaag ttaatcccat agatggaaca gaattaactg ttttaggttt
      541 agttggagat actagtaaaa tagatgccaa aagaatagaa gcaaataagg tagttgaaaa
      601 agttatgcat gtggtagagc cttttaaaaa ggccaataga aaatttcatc cagaaccttc
      661 aatcataaat gttaatggaa tggaaattgg ttcaaagaaa attgctatga tagctgggcc
      721 ttgttctgtt gaaacagaag accaaatagt atctatagct aaagatgtga aaaaatcagg
      781 tgcaggattt ttgagaggtg gagcattcaa accaagaaca tcaccatacg ctttccaagg
      841 attgaaatat gatggattgg atttattaaa aaaagcaaaa gaaaaaacag gacttcctat
      901 agttacagaa ataatgtcaa cacaagatat agatatattt gaagaaaatg tagatgtaat
      961 tcaagttgga gctagaaaca tgcaaaactt tgatttatta aaagaattag gtaaaacaaa
     1021 taaaacaata cttttaaaaa gaggattatc tgctacaatt gaagagtggc taatgtcagc
     1081 agagtatata atggctggag gaaatgaaaa tgtagttctt tgtgaaagag gtataagaac
     1141 ttttgaaaca tatactagaa atactttgga tttaagtgca attttagctg tgaagaaact
     1201 tagtcattta ccagttatag ttgacccaag tcatgcagca ggtaaatcat ggatggttga
     1261 ctctttatct aaggcagcaa ttgcagttgg agcagatggt ctaataatag aagttcataa
     1321 tgacccagca catgcacttt gtgatggaaa acaatcgatt aagccaaatg aatatgatga
     1381 attgataagt gaattaaaga ctatcgcatc agcagttggt agagaaatat aatctataaa
     1441 aggatggtta aacgtggaag aaaaactatt agtaaattta aaagaaaata gctacaatat
     1501 atttattaaa aaaaatatac taaagaatat aggagaagaa ataaaaaaaa tatattttgg
     1561 aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg ataaagtaaa
     1621 aaataattta agtgaatctg gatttgaaat taaatcttta atattaaaat ctggtgaaga
     1681 aacaaaatca tttaatactc ttcctaaaat atacgatagt ttacttgact ttaagttaac
     1741 tagaaaagat ttgattatta ctttaggagg aggggtaata ggtgatttag gtggatttgc
     1801 agcagcaact tttttaagag gtataagttt tattcaaatt ccaacatctt tattagcaca
     1861 agttgatagt tctgtaggtg gaaaagttgg agttgacctt gagagaggca aaaatttagt
     1921 gggaagcttt taccaaccaa aggctgtgtt tatcgatcca aatgtattat tatcacttcc
     1981 agaaaaattc tatagagatg gaatggcaga agttataaaa tatggatgta taaaagacaa
     2041 aacttttttc aataaacttt taagtttaaa aaatagagaa gaagtaatgc aacatataga
     2101 atacattata cataaatgtt gctttattaa aaaaactgta gttgaagctg atgaaaaaga
     2161 ctttggagat agaatgcttc taaactttgg ccatacatta ggtcatgcta ttgagaaata
     2221 ctacaatttt acgggttata cccatggaga agctgttgcc ataggaatgt ataagataac
     2281 tttagaaagt gaaaagatag gaattacaaa aaaaggtaca tcagaactta taaaatcaat
     2341 tttaatgaat tataatttgc cttatgagat tgaattaaga gataataaat ctatagtaga
     2401 tgctatatct cttgataaaa aaaatctagg tgatacatta aaaataatac ttttaaaaga
     2461 tattggagag agtattatat ataatactac ttctaaattt tttcaggagg taagataatg
     2521 ggaagtctaa aaatatatcc aagtaaatta tctggagatg taaaaatacc accatcaaaa
     2581 agtatggcac atagggcagt tatttgttca tctctaagca atggaaaaag tagaatttct
     2641 aatatagatt tttctgatga tataatagct actataagag caatgacatc attaggagct
     2701 attatagaaa agaaagaaga tattttagaa atttcgggaa ttttttctaa agaaggtatt
     2761 ttaaatagag agaatcagtt gaatcaacct aaattgacaa tagattgtaa tgaatcaggc
     2821 tctacattga gatttttagt acctatatct ttaacatttg atggtgtaaa aaggtttata
     2881 ggtagaggta atcttggaaa aagaccactt gatacctatt atgagatatt tgacagacag
     2941 aatataaaat atagttacaa agaaaatcag ttagatttga ttatatcggg taagcttaaa
     3001 ccagatgaat ttagagtaaa aggaaatata agttctcagt ttatcacagg tttactgttt
     3061 atattgccta cgctagaaag tgattcaaag ataataatta ctactgagct agaatctaaa
     3121 ggatatttag atttaactct ttcaacgata aaagattttg gggtagaaat tataaacaat
     3181 aactataaag aatttattat aaaaggaaat caaacgtata aagcgagaga ttataaagta
     3241 gaaggagatt attctcaagg agcattttat ttatcagcag atgctatagg agaagatatt
     3301 agcatattag accttaaaga agattctctt caaggggata gtgaagtagt tgaaatttta
     3361 tctaggatgg gaatggaaat attaagagaa ggcaacaaaa taaaaggaat aacaaatggt
     3421 ttaaatggta ctttgataga tgcatctcaa tgcccagata taatacctgt attatctgta
     3481 gtagcatcct taagtatagg gaagactaca ataattaatg ctggtaggct tagaattaaa
     3541 gaatgtgata gacttcatgc aataaatgtt gagttaagta aacttggagc aaatatagag
     3601 gagaaagagg atagtttgat aatcgaagga gtaagcaagt taaatggtgg agtagaggtg
     3661 tggagtcaca aagaccatag aattgctatg acacttgcaa ttgcttcttg tagatgtgat
     3721 aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat cttatccaca tttttttaaa
     3781 gattttaaga tgttaggagg tcgaattgat gagtggaata tggggaaata atttaaaagt
     3841 atcaattttt ggagaatctc atggaaatgc tataggtata aatatagatg gtcttccttc
     3901 ggggattgaa cttgaccttg ataagattga taaagaaatg aagagaagag cacctggaaa
     3961 aaattctata tctacttcaa gaaatgaaag tgatatacca gaaattctaa gtggatattt
     4021 taatggaaga actacaggaa cgccattatg tgcaatcata agaaatagtg atactcgttc
     4081 aaaagattat ggagagctta aaaatttaat gagaccagga catgcagatt tcacaggtaa
     4141 tgtgagatat tctggattta atgactatag aggtggaggt catttttcag gtaggataac
     4201 agctccatta gtattttgtg gggctatatg taaacaaatt ttatcacaaa aaggaataga
     4261 gataggagca cacataaaaa aaattaaaaa tatagaagat atgagttttg attatgtaaa
     4321 tatttcaaaa caacagttat caaatttgca aactttggaa ctacctttac ttgatttatc
     4381 aaaggaagaa gccatgaaaa atacgattat agatgcaaaa aatcaaggag attcagttgg
     4441 tggtataatt gagtgtgcag tagtaggtat taatgtaggg cttggaaatc cattttttga
     4501 ctctgttgaa tctactttat cacatttgtt gttttctgtt cctgctgtaa aaggtgtaga
     4561 gtttggttta ggttttgaac ttgcagatat gtatgggtct caaagtaatg atgaaatgta
     4621 ttatgaagga aatcaagtta agagcaaaac aaataacaat ggaggaatta taggagggat
     4681 tactactgga atgccaataa ttttcaaagt ggcaattaag ccaactccat caattagcag
     4741 acaacaaaac acagttaata taaaagataa aaaggacgat attttatata ttaaaggcag
     4801 acatgaccct tgtatagttc aaagggctat acctgtaata gaagcagtaa cagccattgg
     4861 aatttttgat ttgatgaaag gacgataata atggaggatt taacgagata tagaaatgag
     4921 attgacaata tagataaaga gcttattcaa ttatttgaaa agagaatgaa tacagttctt
     4981 gaaattgcca gacataaaat gaaaaataac actactatac ttcaaaaaga tagagaagaa
     5041 aaagtactaa gtagagccgt agataattta gataataaag catattcaca agaaacggta
     5101 cattttttta attctataat ggaaattagt agaggattac aaaaaagatt gatagataat
     5161 gatggtgagc agaaaatcga gtttagttta gattcatcaa ataagaattt tgatttgtca
     5221 aatgtaaata aatataagtc tttaaaagat gaattaaata aaaaaaatat cctagtagga
     5281 tttccaggaa aatcaggttc atttacagaa gaagcactaa ataaattctt caacaaaaaa
     5341 atatcaaaaa aacaatttaa agaatttgaa gatgttttca tagcacttaa aaataaagaa
     5401 ataaactatg gaataatccc catagaaaat tcatctacag gagctatctc tgaaacttat
     5461 gaccttttga gaaagtacgg tttttatata gttggagaag aatgtataaa aatagaccaa
     5521 aatttaatag ggataaaaga cacaaagctt gaagatataa aagaaatata ttctcatcca
     5581 caaggtatag gacaatgtag tgaattttta aaacaaaata gtacatggaa attgatacca
     5641 tttaataaca cagcaacaag tgcagaactt gtaagaaaac ttcaagataa aacaaaagca
     5701 gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag aaataataag tccttgtata
     5761 aatgatatag caaacaacta tacaagattt gtagtaatca gtaatcaaat acacattgaa
     5821 gaagaatcaa acaaaatgag tgtagtattt tctgttgaac atgaagcagg aaaactttat
     5881 aaagtacttg gatactttgc taaaaataat ataaatatga caaaaataga atcaagacct
     5941 atgaaaaatg cctcatggag atatttcttt tatatagatt ttgagtgttc tatatacaat
     6001 gctaaagtgt acgatttatt agaattgata gaacataata cagcatattt taaatttatg
     6061 ggagtatata aaaataaaac tgaatagata agataaattt ttatttaatt tataattatt
     6121 aggaggtgta agtatgaatt ttttcggtct agtaggtgaa aaactctctc acagtgtttc
     6181 acctcaaatc cacaagagag tattcgagat attgaatatt gaaagtgctt ataaaaactt
     6241 tgaaatttca aaagaggata tatctaagct agacggtgct ataaaattat taggaataca
     6301 aggagtaaat gttactgtac catataagga gcgtattatg aaatatcttg attttatatc
     6361 accagaggca aaacgtatag gagcagttaa tacaatttta cttagagaaa atatgttata
     6421 tggttacaat acagattact ttggtcttga tagtatgttt aaaatggcta atattgatgt
     6481 tcaaggaaaa gtagcagtta tattaggaac tggaggtgct tctaaagctg ctcttacata
     6541 ttttattgat tctggtatag aaaagcttta tgtttcaact agaaaaaagg atgataaaaa
     6601 attattaaat agtaaagcta tattaataga ttatgaagaa cttaaacata taaaaggaga
     6661 tataatatta aatgctacac cagtaggtat gtaccctaat ataggaattt caccagtgtc
     6721 aaaatctatt atacaaaatt ttgatatatt aatagattta atttacaatc ctggagaaac
     6781 agagttttta agaattggta atagtatggg taaaaaaact tgtgatggat tatatatgct
     6841 ggtagggcaa gcaataaaat ctcaggaaat ttggcaagat actaaaatag ataatagtat
     6901 tttagatgtt atatataatg aactaaaatt agaatttttg taagggagat tgctatgata
     6961 aataaaacaa aagaaaaatt gatattaata ggtatgccag gtagtggaaa aactacaatt
     7021 ggtaaacttt tggccaaaga atataattgt agtttctgtg atatggatga ttatataatt
     7081 caaatatcta aaaaaagtat agcagagttg ttttcagaag gagaagatat ctttaggaac
     7141 tatgagactc aagcatgtag agaactttct ataagtgata aaacagtgat atctacaggt
     7201 ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa aagaaacagg tataataatt
     7261 tttattgata gacctataca aaagattttg gaagatataa atattaattc aagaccatta
     7321 ttaaaaaacg gaaaagatag attgtataat ctttacaatg agagaataaa tctttataaa
     7381 aaattttcag atatagagat tttaaatgat aaatcattaa ataatgctgt atataatatt
     7441 acaaatgcag tcagtgaaaa ttttaaattt gactttaaag aaaaataatc tagggggaaa
     7501 taaaaataat gaatattgtt attgtaggac taggagttat aggaggctct tttgctaaag
     7561 cattaaaaaa agctggatat gaaaatgtat ttggagtaga tgtagattta gaaactttaa
     7621 aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac tacaggtaaa gagttattta
     7681 aaaaagctga tttaattata ctatctatat atcctagatt agttgtagat tttctaaata
     7741 ataataaaaa cttttttaaa aaagggacta taattactga tactacagga ataaaagaaa
     7801 cacttatcaa tgatgtgttg caaattattc cagatgatat agattttata tttggacatc
     7861 caatggcagg tagggaaaaa aagggtattg attttgcaag tgaacaagtt tttaatggcg
     7921 caaattatat aattactcca actggcagaa acaacataaa aaatttagag ttagttgaaa
     7981 atttaatttt agagattgga tttaaaagag taaaaaagtt aacttcacaa aaacatgatg
     8041 agattatagc ttttacttct cagttacctc atgttatggc agttgctctt ataaatagtg
     8101 atgaagaagg tagagatacg ggtaaattta ttggagatag ttatagagat ttaactagaa
     8161 ttgcaaatat gaatgaagac ttatggagtg aacttttttt aggaaataga gataatttac
     8221 taaaagttat agaaaacttt gaatcagaag taaatttagt aaaagaagct atatttaaca
     8281 atgataaaaa taaattaata gagtatttta aaaaatcatc aataagaaga gaagctttag
     8341 aaaaataaat attaatattt aagctatata tagatgggta taaatataag cgatagtgta
     8401 tttaattgta ttaataaata tggattcaat ataattaaat atgctattga tatatttgta
     8461 cccttaacta ttgttatctt ttaaatgata atgatattta tttaaatact ctttattaac
     8521 aatttataaa acttaattgt aaaaaatact tattaatagt aaaataaagt gaatcaatta
     8581 tattataatt gctaaagaga gtgaggtgta gctatttgaa aaaaaataga ttaatagcaa
     8641 agaatatatc aatagcattt atagtactat tcttttttag tgtatttaca ttcttttatg
     8701 taggtaatat taatagagtg ttagaatatg aaacaaatga cataataaca gttacaattg
     8761 ctggatggat tatactttca tttttatttt taggtattat aatttacata ttatattcaa
     8821 aagcaaatag tcaaaaaact atagaaaaag tagcatatac ggattttgta acaggttatt
     8881 ccaactggag aaagtttgaa ttagatgtta caaatttatt aaagaaaaca tcacaaaata
     8941 ataaatatgc aatggtcata ttcgatattg ataaatttaa agcaattaat gatatatatg
     9001 gtcataaaaa gggaaattta atattaaaag atattgcaga tactttaaat gaattgacta
     9061 atatcaatga aacttttgct cgtgtaagtg ctgataattt taatatatta ttaacatata
     9121 acaaaaaaga ggatataata aatattatca aaaagattat ggcaaataat gaacttgtaa
     9181 acttatcttt tggaatttat gaaatcaaag ataaggattt atctgtaagt gtatatagcg
     9241 atagagcttc acttgcaaaa tcatcaataa aaaataatag tgatgtaaat tttgcatttt
     9301 ttaatgataa attaagagag aaattattac ttgaagataa gatagaaaaa gaaatggaat
     9361 atgctcttga aagtgggcaa ttcgtaatgt atttacaacc aaaatacaat attaaattag
     9421 ataaattttg tggaagtgaa gccctagtca gatggcaata tacagaaaaa gaggtcatat
     9481 atccaggtga ttttattcct atatttgaaa aaaatggatt tattagaaag attgatatgt
     9541 acatattaga acaagcttgt aaagaaatcc ggtctttatt tgataaagga atttcacctt
     9601 tacctatatc tgtaaacttc tcaagagttg atttttttaa aaaagatttt attgaaaaca
     9661 tagtaaatat atgtgataga tataaaatac catatagttt aatagaaatt gaaattactg
     9721 aatctagtat gtttggagat actgatactc tatttaatgt tagtagaaac ctacaggata
     9781 ttggctttat agttgcaatg gatgattttg gctctggata ttcttctgta aatatgctta
     9841 aaaatatacc attaaatgta ataaaattag atagaggatt ttttgttgat gataaagatg
     9901 ttgataagag tcaaattgtt ataaaaagca tagtttctct cattaagcaa ttaggaataa
     9961 gggttgttgc tgaaggaatt gaaacaagaa gtcaaataga aatgttaaaa aaagcaaatt
    10021 gtgatattgt tcaaggatat tatttttcaa aaccattacc aattaaagaa tttgaaaaat
    10081 tagtctataa aatatagtca gcgagtctta aatgggaggt ggtgattaag tgataagagc
    10141 aaaatcgtta aaaaatgtac tgagtattgt tttaatattt ttatttacaa taatttcttt
    10201 ttctgatttt gcatttaaaa ctaatgtcca ggaagaagaa aatagtaaaa ttaatttaag
    10261 agaaatatcc ctacaaagtt ccaatgctat aaaaataaaa ttagatagtt ctctatcctt
    10321 tattaaaagt gtggcaaata tggtttctta ttttaatact atagataact caaaaatagt
    10381 tccattatta gctaatatat caaaagaatc tgactttcaa agaatctcta ttgtatctcc
    10441 tgatggaata agtcatacca gtgatggcaa aattatcaat gtgtcaaagt atcagtattt
    10501 taagaaagct attaaaggag aaagtaatat atctgaagtt gtgaaatctc caattgatgg
    10561 aaaagatact tttgttatag ctactcctgt atttaataag aataacaaaa taattggtgt
    10621 tttatacggt tcttactaca atgataaatt agctgaattt atagatataa caagttttaa
    10681 tagtgaaggg tatatagata tatttgaatc tagtggtcaa tttgttttaa aatcttcaca
    10741 tacaaacaaa ctgattaagt caagtagtaa catatatgat atatttaaaa aagcttcttt
    10801 tgatgatggt tatagctata aaaaaatata tgaagataca aaaaataaca agagtggctt
    10861 tacctcatat gaatacaaag gagaaaatcg ttatgctact tatacaccca ttggtgtaaa
    10921 tgactggtat gtaatttctg tgataccaca atatactgta agaagaaaca ttgataaaat
    10981 aaatcaatta gcattttctc ttatttttaa ggtattctta ttgtttatta tgcttataat
    11041 atatattatt tataaaaaca ataaatctca gaaagatata ctaaaagcta acaaagaaat
    11101 ctcatctatt acaaatagca ttcctggagg agtacaaaag tgtaccctgg gtgaaacatg
    11161 caatattaat tttttaagtg atggatttat agagttgttt ggttatacaa gagaagaaat
    11221 aaaaacattg tttaataatg atttttatag tttaatacat aaagatgatg ttgaaagaat
    11281 aaaaaaagaa atttttaaac aactttacaa aaataaaact attgaattag aatataggat
    11341 tataacaaaa aatggtaata cagtatgggt tttagataaa ggagagctta taacagaacc
    11401 tgatggaaaa gaatattttt gttgtgtaac tgtagatatt actagaacaa aacaattaga
    11461 acaagagatg gaacaaaaaa atctagaact tgaaatgata tcagaaaatg ttttaggtgg
    11521 tataattata acagaattta atgatgattt tactataaaa tacgctaact ctggttatct
    11581 ttcaatgatt ggatatacac gtgaacaatt agaaaatgaa cttaaataca aagcttgtaa
    11641 tcttgttttt tatgaggata aaaagaaggc tcttgaaagt atacaacatc aattaaacac
    11701 aaaaggaata gctgtatatc aacatagaat aagaagaaga aatggtaata ttatttgggt
    11761 ttctataaaa ggaaggctta ttgttgatga tgatggtatt gaaaaaggta tttggattct
    11821 aaatgatata acagatttaa aaaatgcaga acaaaaactt aaaataaatg aagaaagatt
    11881 tagaattgca ttaaagcaaa catcaaacac tatatttgag tatgatataa aaacaaagtc
    11941 acttttaaat actagtaaag agcttgataa atataatatt tataaaatta ctaaaaacgt
    12001 tccatattct ttagtagaat caggagttat tcataaagat tttagtgatg attttataaa
    12061 catgtatgaa aatgtaataa atggagcaaa aaaagcaagt tgtgtagtta agatgaaact
    12121 tccagatgaa aattatatat ggaataaagt taccttgact actatttttg atgaagatgg
    12181 taatcctgta agagcaattg gtatacagga agatataagc aaacaaaagg aagcagagat
    12241 aagatacaat caagaaggta agtatcattc agctatattg tcggaagcaa tgataactta
    12301 tgaaataaat gtatcaaagg atacttttat caaaggaaat gaaagttggc aaaaaccatt
    12361 tggaattaag cctgacaata actattctca aatggtaaaa actatagcaa atagtattgt
    12421 acataaagac aataaagaaa catttttaaa tacattttta tgtgaaaact tattggatgc
    12481 atataaaaat aatgtgactg aaataaaatt agaatatcaa agaatgaatc aagaaaataa
    12541 aatgatttgg gtcatttgta caatgcactt aataaaagat tctattacga acgatataaa
    12601 agcacttgct tatgttaaag atattgacaa gcaaaaaaat gaagaaatta gattaaaatt
    12661 aaaagctgaa agagatactc ttacaggatt atataataaa ggtactacag aaaaattgat
    12721 taatgatttc ttgaactctg aggaagcaag tttaggaatg catgcatttt taattgttga
    12781 tatagataat tttaaatctg tgaatgataa tttaggacat gtatttggag ataaagtatt
    12841 atttgaagtt tctaaaaacc taaaacctat atttagaaaa gatgatatag taggtagaat
    12901 tggtggagat gaatttatag tattcttaaa aaatattcag tcttttgaga atatcaaaaa
    12961 gaaagcagaa aaaatatgcg atatatttag aaattcttat acagaaaata aaaagacata
    13021 taaaatatct ggaacagttg gaatttcatt tgtaccagaa catggaactg tatttgaaga
    13081 actttataaa aaagcagaca tagcacttta ttatgcaaag agcaaaggaa aagataagta
    13141 tgcactttat aatgattgta tctaccaaaa tgaacttgaa aataatataa gtgataataa
    13201 tataaaatta gcagataaat gtgagaaatc caaaccagta cttaaagaaa ttcttgattc
    13261 tattgataat tatatctata tagtgaatcc aaatacttat gaacttcttt ttgtcaataa
    13321 taaggtgtct cgtttggatt tagagataaa attaggagaa acctgttata aatcactatt
    13381 aaaatctgat agaccatgta ataattgccc tcttaacgga ttagaagaaa ataagcattc
    13441 aaaattcaaa atagagacaa atacatttgg taaaaatatc agtatagcaa gttggattaa
    13501 ttgcatagat ggagaataca actgtcttat agagtgtata gattcttctt tatacaaaaa
    13561 taaataaaaa aggactatga agatatgtta aatagtataa ctttgatact tggtataata
    13621 tttacaattt actattttta tctcaaaata gtttttggag gaatttcatt tagtgagatt
    13681 ttcttagttg taggaattgt actaattatc tatcaaatat ttaagaaaaa aattaaagaa
    13741 aagaaggttt tttatagggt gttaaaaata ataatcagca tttttttaat agtatttgta
    13801 gttactgaga gtctaattat tttttatccc aaaaatagtt tagaatcaaa aagtgattat
    13861 ctcttaattt taggagcttc tgtaaaaaag actacaccaa gtacaaccct gaagggtagg
    13921 ttagacactg cattgaaata cctaaaaatt aatgataatt gttatgtggt agtatctggt
    13981 ggtaaaggaa gtggagaaaa aataactgag gcaaaagcta tgaaagatta tcttataaaa
    14041 aatggtatag ataaaaatag gataatagaa gaggataaat ccacgaatac ttatgagaat
    14101 tttaaatatt caaaattgaa gatagaagaa catagtcaag gaaaactaag cgatttaaaa
    14161 gtaaaaatag taacaacaga ttttcatgtt ctcagaagta aaatattagc atatagaaat
    14221 ggatataaaa atacgtcttt ttatgctagt aaatcaaaac tttcatttgt accaacttac
    14281 tatacaagag aattttttgc attatggaaa acaatcattt ttgatagata ataaataaaa
    14341 agactagata taaactcata tctagtcttt tctattccct taattctaat ccattttcca
    14401 actttacttg ttgtagaaat ttcaacattg ggatttacaa gtaattgctt ataagaagtt
    14461 ttatatttta cgattccaaa atataatttt ccttcataat ccattataaa tccgaatggt
    14521 cttactcttg gtttattatc ttcaactgta gcaaaataga atgttgagtt ttctttaaaa
    14581 aaattcatta ttttttcatg attaaatcct ctttgtatct tatactaata taatgaatat
    14641 aaataaaata ttcataatac taatataaat cgatataaaa acaattacaa tattatattg
    14701 attattcaaa attatatata aaattcagaa aaaaatttta gtttacataa taaaaataaa
    14761 gtcaactaaa attattgatt ttagattaaa gtaccggatt aagcatttaa gtagatttta
    14821 a
//
LOCUS       DAEMHZ010000104        14702 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000104 DAEMHZ010000000
VERSION     DAEMHZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.104"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1991
                     /locus_tag="KPK72_002792"
     CDS             <1..1991
                     /locus_tag="KPK72_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG2489990.1"
                     /translation="VSKNIKKTALELHDTNEELTKKTESLKEKNKKLIYETEKIEELK
                     GILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNTFGDYKEQDKLTDKIKNFN
                     DLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQTEGVLCVLEDKTKKKEYE
                     RKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIFKNNLESIDFSLVCKSEES
                     IEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRKISQIKLEESQSSYSRFID
                     ILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIKLLMNANTEEKMKFTDNMA
                     KVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRYIMLIIKDLVHKKYSEQAE
                     KELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDSFYRNEKIKDYNNNIKSHV
                     DLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSVIEDLFMNIEEYALRKDIK
                     ILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWIYVSIHYKIDKVIISVKDT
                     GVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNLVSLHGGDIEVVSEVNKGT
                     EFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(2110..3054)
                     /gene="manA"
                     /locus_tag="KPK72_002793"
     CDS             complement(2110..3054)
                     /gene="manA"
                     /locus_tag="KPK72_002793"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG2489991.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            3227..4588
                     /locus_tag="KPK72_002794"
     CDS             3227..4588
                     /locus_tag="KPK72_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2489992.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLNINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TLEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            4676..6565
                     /locus_tag="KPK72_002795"
     CDS             4676..6565
                     /locus_tag="KPK72_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2489993.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            6580..7035
                     /locus_tag="KPK72_002796"
     CDS             6580..7035
                     /locus_tag="KPK72_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2489994.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            7094..7411
                     /locus_tag="KPK72_002797"
     CDS             7094..7411
                     /locus_tag="KPK72_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2489995.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            7436..8698
                     /locus_tag="KPK72_002798"
     CDS             7436..8698
                     /locus_tag="KPK72_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2489996.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            8803..9867
                     /locus_tag="KPK72_002799"
     CDS             8803..9867
                     /locus_tag="KPK72_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2489997.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            9921..11099
                     /locus_tag="KPK72_002800"
     CDS             9921..11099
                     /locus_tag="KPK72_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2489998.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(11290..12348)
                     /locus_tag="KPK72_002801"
     CDS             complement(11290..12348)
                     /locus_tag="KPK72_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG2489999.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            12415..12786
                     /locus_tag="KPK72_002802"
     CDS             12415..12786
                     /locus_tag="KPK72_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG2490000.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            12823..13497
                     /locus_tag="KPK72_002803"
     CDS             12823..13497
                     /locus_tag="KPK72_002803"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG2490001.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKDFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            13746..14585
                     /locus_tag="KPK72_002804"
     CDS             13746..14585
                     /locus_tag="KPK72_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2490002.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
BASE COUNT         5859 a         1546 c         2517 g         4780 t
ORIGIN      
        1 tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat gaagaattaa
       61 caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag acagaaaaga
      121 tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct acattagatg
      181 tagcagtaaa tagcatagta gtatttagca aagatggaga aattacatat gcaaataaga
      241 gctttagaaa tacttttgga gactataaag aacaagataa attgactgat aaaataaaaa
      301 acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag aatgcagaga
      361 aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct ttaattattg
      421 cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa aaggagtacg
      481 aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt attggagatg
      541 gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt agggaaattt
      601 ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa gaaagtatag
      661 aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt tctcagtata
      721 ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct cgaaaaatat
      781 ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat atattacctg
      841 atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag tcgtttttag
      901 atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa ctattaatga
      961 atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt ttaaatgaaa
     1021 atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat atagtagaag
     1081 tagaattaag tgcattacct tttgctatat acaatactag atacataatg cttattataa
     1141 aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt gaaagattta
     1201 aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaatta aaaaccccac
     1261 taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat gaaaagataa
     1321 aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt tcttataggc
     1381 ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg ttctatacac
     1441 taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg aatatagaag
     1501 agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa gaaattaatg
     1561 tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca aactgtatta
     1621 agtttactga taatggaggc tggatatatg taagtattca ttataaaata gataaagtaa
     1681 tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagaa ttgatttttg
     1741 aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt ggaatagggc
     1801 ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta gtaagtgaag
     1861 tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt agtgaacatt
     1921 acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa ttttctgata
     1981 tatactatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg tttaaatatt
     2041 taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat ttttagctag
     2101 gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac taaaatctcc
     2161 aaagtcgttt ggtagtataa aatgcatacc cttttttaaa ttgtattcta cagaattgtg
     2221 tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat tgtgtgtaaa
     2281 attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg acacaaatgt
     2341 tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat ttatatgagg
     2401 aacattcgta acatctattg atttttgaac atgaagttca cgcttattac ctgaattgtc
     2461 agttctatca taatcataga ctctataagt agtatctgag ttttgttgag tttctaaaat
     2521 caatgttcct ttgcatatag catgtatagt accacttgga acatagaaaa agtctccctt
     2581 ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca tatttacaaa
     2641 ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag aacaatctat
     2701 tacataccaa cactctgtct tacctaattc tccattttca ttttttttag catattcatc
     2761 atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg taagtaaagg
     2821 aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca attcagatag
     2881 ttttttacct ttatattttc cattttctat taagcaatct ccatttttat gagagcttat
     2941 agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat ctttaagtgc
     3001 agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt ccataatcct
     3061 gttcctccta tgatattaaa tttatcctac aaataattat atataaatag attataaaat
     3121 caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt attaaataag
     3181 ataaactatg atatataata taaagttcta aattggagtg ataattatgg agttgtttaa
     3241 ggcagaaagc aaaaaatata aaaaagaatt tttaaaatta ttggagcaat gggttagtat
     3301 accttcattt tatgatagaa aaactgtatc taaagacatg ccttttggaa aaggagtata
     3361 tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag taaaaaatat
     3421 agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gagtatgtag atatatttgg
     3481 acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta aactaaatat
     3541 tataggtgat aaattagttg ctagaggtgt atctgataat aaagggccta tgatagtgaa
     3601 ttttttagca ctaaaaatga taaaggattt aaatattaat ttaaaaagaa aggttagatt
     3661 aatagctggg ggaaatgaag agagtggatt taaatgtata aagcattatt atagcaaaga
     3721 gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa atggagaaaa
     3781 aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat atataagtgg
     3841 tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag aaaaattggg
     3901 taaagataat atatgttttg atattaacaa tatatcgata aattatgata atggcaatta
     3961 tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa atcctatatt
     4021 agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag atttatataa
     4081 gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg aggggaaatg
     4141 tgggatttta tcaatggtgc caacaataat aaatatagta gatggaaaat tagaagtagt
     4201 tttaagcgtt agatatccag aaatattaac ccttgaagat ataataaaaa aatttaattt
     4261 gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt taaagcaagc
     4321 aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat atataaagta
     4381 ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg cttctgaaat
     4441 gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca atgtccatag
     4501 tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat atgcagaggc
     4561 aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag acatattata
     4621 tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg atacaatgat
     4681 aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat atatgacatc
     4741 aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat atatagcaga
     4801 tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag gtataggata
     4861 caagttaagc ggttctaaaa acaatatagc cagagctatt aaagaagcaa ataaatatat
     4921 tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat gtatgcttat
     4981 taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa gtatagccgc
     5041 tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact tagttataaa
     5101 atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa ggcaattaat
     5161 tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt tagatgacat
     5221 tagtgaaaac gatatacact gtatagaaag tattttagaa aagcacttaa gagaaaaaga
     5281 tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg tttctgtatt
     5341 taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt atagatttca
     5401 taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg gatttaaact
     5461 tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct ataaaggtac
     5521 acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta taagtagtgc
     5581 acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg agtttatggt
     5641 tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg taaaatcaaa
     5701 taatcctttg cttcatcaaa taaaagagaa gtttcctata gcttttaatt tagccgtgtt
     5761 tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg agcttggata
     5821 tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta gtaagaaaat
     5881 ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa agctaaaaca
     5941 gaatataagt gatttaagtg ttgtgggtgt ttatccagtt agatatctcg atatggctat
     6001 aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg aaaaacctgt
     6061 cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca aagcctttta
     6121 tgaaaaggaa gaaagaagaa agataataag caacatgttt gatgaaaaag cttttttcag
     6181 tataaaggct aatactaaag aagaggtcat aatttcactt tcaaataaac taaaagaaag
     6241 agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata tttcatcaac
     6301 tgaaatagga aatctagtag caatacccca cacaatagtg aagggagata aaaaatctat
     6361 aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg ttcagctagt
     6421 atttatggta ttttttaata ctaaagaaaa acataatttc tcgatattta aataccttta
     6481 taattttata aaagatgaag gcggagtaag aggaattatt aaaatatgtg actttaataa
     6541 attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa catatttaac
     6601 aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt aaaatttata
     6661 tcaaaaaaag ctaaagactt aaacttggca gaagatgaga gcttagtata tgaaggttta
     6721 atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat acctcacact
     6781 aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga agatgttgtt
     6841 tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc aggaaaaagt
     6901 aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat aaataaagaa
     6961 tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt aataaatgaa
     7021 gctttaggtt tataattaaa cactaaggtt ataaaataaa aaaataatta actaaaaata
     7081 ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgtccaa tgggactagc
     7141 tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag gttatgaagc
     7201 aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag atgatatatt
     7261 aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg aaagatttga
     7321 tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag aaggtgttat
     7381 aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa atagaatggc
     7441 aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa ataatgtagg
     7501 aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga aagaaataag
     7561 taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg gtggacttat
     7621 aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat ctgtaggtat
     7681 agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt taaatatagc
     7741 acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac tgtttgcagc
     7801 attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta taggtggata
     7861 tatagctaat aaacctgtag gtgtaccaca attcgttgat ggacaatgga ctgaagttgt
     7921 tccagttgca tcaggatttt taggtgcaat actaatagca tttataatag gatattttgt
     7981 aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta aaacaacatt
     8041 tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta taataactcc
     8101 atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag gagcagctgg
     8161 agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag gaggaccaat
     8221 aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac ttccaataac
     8281 agcaagaaca atagctatag ttgttcctcc aattggatta ggattaacaa cattattaga
     8341 taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag gaaaaacttc
     8401 tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac ttgagagacc
     8461 aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta caggtataat
     8521 tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc tagtagataa
     8581 cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag taggaaatat
     8641 attctataga aataaattaa taaaagatgg taaacttgtt gtagattata tcgactagta
     8701 tttaattttt actatctata aataactttg gctggtaata tggttttaat caatattacc
     8761 agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa gatagaaaaa
     8821 gtaagagagt attttagaga atatgatata gatggatttc ttataaatag tcctacaaac
     8881 aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac gaaagaatca
     8941 caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc tagtttgttt
     9001 aatgtagtat taatggacaa aactagaact cattttgata taattaatga tatttgtaga
     9061 gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga tttatataga
     9121 agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac attaagggaa
     9181 acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt tgatgctaca
     9241 ttttatcata tagtagactt tataaaggtt ggtatgactg aaaagcaagt tgaaaatgaa
     9301 attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac aatagttgca
     9361 tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaagttat agagtatgga
     9421 gattttgtta cttttgactt tggtgctaag tataataatt attgttcaga tattaccaga
     9481 accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat agtaagaaaa
     9541 gctaatgaag agtgtataag ggttttaaga ccgggaatga ctactggaga gatagataaa
     9601 gttgcaagag acataattgg ttcatatgga tatgctggta attttggtca taatctgggt
     9661 catggtgtag gaattatggt tcatgaatat cctgcattag ctcctgaatc aaatgaagta
     9721 ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag tcttggaggc
     9781 gtaagaatag aagatgatgt acttataaca caagatggat gtatgagatt gactacatct
     9841 actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta taacaataag
     9901 aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga gtaagtgaaa
     9961 agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt agagaagatt
    10021 ttgtaccaat gtggattgct gatatggatt ttaaaatacc aacagaaata gaaaacaaat
    10081 ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat gaattttatg
    10141 attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa gaatggatta
    10201 cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt tgtaagaaag
    10261 gtgatagtat aatattaaac acaccagttt atgacccctt tgaatcatca gcaaaaaaac
    10321 agggtgtaaa tgtaatttgt aatacactag atgttgtaaa tgatagatat tatataaatt
    10381 ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg ttttgtacac
    10441 cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta gcaactatat
    10501 gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat attcattatg
    10561 gtaaatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt attctactga
    10621 catcaccaaa taaaggattt aatcttggtg gattaaagac ttcttattca atagttataa
    10681 ataaggatat aagggacaaa tttagaaata aattaaagca aaattctata acttctccaa
    10741 atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag tggttaagtg
    10801 gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta aataaatata
    10861 ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat ataagtggat
    10921 taggtattag tgcaagtgag tttacagata aattggctat agatacagga gtattattag
    10981 aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat ttaggaactc
    11041 aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag tctttataaa
    11101 aatttggttg aattagtatt ttatataaat ttaaactatt aatatggtat atacataagt
    11161 agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt aaatttgcat
    11221 tagtcatgtg ataactattt aaattcatat gtattttgta tgtataatag atataattat
    11281 tatttaaact tattgtttat ttaatttttc aatagaaaaa gatttatctg taagtttaaa
    11341 attatctttt atacccttag ttaggtatac tttcttatcc ttagttatga atatagcatc
    11401 tacattatca attttattaa ttaaatccat acctttttca agaccaagtc caaatgtaga
    11461 agtggataag gcatcacaat taatagattt atcagagatt attgtaacac tacttaaatt
    11521 gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt ttccgttttt
    11581 ttcgataaat ctttcataaa ttccagaagt aacaacagat ttatttgtgg tttctatgtt
    11641 tcctatggat tcaccatttt tactagttgg gtcttgaatg cctattttaa aaggtttatc
    11701 atttttacca tcttctaaag taaatatatt tcctcctaag tttactaaac ctttttttac
    11761 atcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag ctatggcacc
    11821 taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt catccaatat
    11881 gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac ttggaacttt
    11941 agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta tgtcaaaata
    12001 tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg tatcatcaga
    12061 aactctgaca aattttttac cagcattatc atttatttta gaaacatcac ttcctggtat
    12121 atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat ttaagatttt
    12181 atcacttttg gattctttta cattaaatac agtaacttcg tttactgtac caaggtagta
    12241 gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa ataaaattaa
    12301 tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg tatattcctc
    12361 ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca aaaaatgaag
    12421 aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttacagcatg ttatggaata
    12481 ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga taataaattg
    12541 tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagagtag aggggggtat
    12601 aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg ccctgataag
    12661 gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg tatacctaat
    12721 agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga tataatttct
    12781 aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa cttaggaaac
    12841 gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa cttaagaaaa
    12901 tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa tatatatgaa
    12961 gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca agacccttat
    13021 catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt gaaaactcca
    13081 ccatctctat taaatatgta taaagaatta aaagatgatt taggttgttt tatacctaat
    13141 aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac tgctcttact
    13201 gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt cactgataga
    13261 gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg gggaagtaat
    13321 gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga agcacctcat
    13381 ccaagcccat tgtcagcaag taaagacttt tttggatgta aacacttctc taagataaat
    13441 gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa tatataaaag
    13501 taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg taactaatag
    13561 aaatatgctt gaatggttga aaatactatg tatgcatatt tctatttttt tgtcaagttg
    13621 acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata aaaaaagaaa
    13681 cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatctta gggaggaaat
    13741 tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca gttgttttat
    13801 cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta gtagtaaggg
    13861 gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta gaatctcaaa
    13921 acattagcta tgattcaaat gatatagtaa ctttagatga aaatacaaag ttatctaatg
    13981 gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact aaagacattc
    14041 catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct aaggttaaag
    14101 aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac aatggaaaga
    14161 aagtagaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat aaggttgtaa
    14221 gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt tcaagaagaa
    14281 tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg agagtggtag
    14341 caactgcata tactggtcac agtataactg caacaggaac aaaacctaag tggggaacaa
    14401 tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct caatttgaca
    14461 tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt gatatctaca
    14521 tgaatgattc aaaaacagtt tataactggg gaagaaaaac tatagatata cgtatattaa
    14581 aatagttaaa taatttatat ttttactgga aattatattt tgattatata gagaagaaac
    14641 tctttataga ttctaaaaat ataatttcca gttttttatt ttggatatgt aataaaacag
    14701 gt
//
LOCUS       DAEMHZ010000105        14629 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000105 DAEMHZ010000000
VERSION     DAEMHZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.105"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(16..267)
                     /locus_tag="KPK72_002805"
     CDS             complement(16..267)
                     /locus_tag="KPK72_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490003.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            356..1087
                     /locus_tag="KPK72_002806"
     CDS             356..1087
                     /locus_tag="KPK72_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG2490004.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            1146..2057
                     /locus_tag="KPK72_002807"
     CDS             1146..2057
                     /locus_tag="KPK72_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2490005.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2108..2920
                     /locus_tag="KPK72_002808"
     CDS             2108..2920
                     /locus_tag="KPK72_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG2490006.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3029..3721
                     /locus_tag="KPK72_002809"
     CDS             3029..3721
                     /locus_tag="KPK72_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2490007.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3808..4749
                     /locus_tag="KPK72_002810"
     CDS             3808..4749
                     /locus_tag="KPK72_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490008.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4786..5943
                     /locus_tag="KPK72_002811"
     CDS             4786..5943
                     /locus_tag="KPK72_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2490009.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6095..6655
                     /locus_tag="KPK72_002812"
     CDS             6095..6655
                     /locus_tag="KPK72_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2490010.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6683..7174
                     /locus_tag="KPK72_002813"
     CDS             6683..7174
                     /locus_tag="KPK72_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490011.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7403..7954
                     /locus_tag="KPK72_002814"
     CDS             7403..7954
                     /locus_tag="KPK72_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490012.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            8040..8972
                     /locus_tag="KPK72_002815"
     CDS             8040..8972
                     /locus_tag="KPK72_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG2490013.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            9027..9890
                     /locus_tag="KPK72_002816"
     CDS             9027..9890
                     /locus_tag="KPK72_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2490014.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            10137..11999
                     /locus_tag="KPK72_002817"
     CDS             10137..11999
                     /locus_tag="KPK72_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2490015.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            12002..13468
                     /locus_tag="KPK72_002818"
     CDS             12002..13468
                     /locus_tag="KPK72_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2490016.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRRGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            13492..14328
                     /locus_tag="KPK72_002819"
     CDS             13492..14328
                     /locus_tag="KPK72_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2490017.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFIFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5762 a         1422 c         2582 g         4863 t
ORIGIN      
        1 aattattttt gataactatc tttttttatt tataaatttt gctcctaaat attttttgat
       61 atcttcttca ggagaaaagt catcaataag tatttctttc ccagtgtctt caaatactac
      121 aagaacttca cagtcacctt gtaagaaaaa ttttctaact gtatcttctt taacttctct
      181 aacttttatt acattctttt tttctttttc aaagtctttt gtgtaataac ttcccatcat
      241 attaattatt tttgtatatc tattcataaa atacctccat atgcgattaa atttaaaaaa
      301 tatgttataa taattgtttg tattaactta ataattataa caaggagtaa taataatgac
      361 tagtataaaa aataaagata ttttaaattg tatagattat tccattaaaa ataagctctt
      421 tgacaaacta aatgatgtat ataagtcctt gccagtagga aattgttctg gatgtggaaa
      481 ttgttgtatg gagtcagtag gtataaattt aattgagttt ttgaatatat tttgttatct
      541 tgaagataga gttgatttaa aaaaaagctg tataagtaaa atattagatt attattttga
      601 ggagtataca agaaagaatc catgtccatt taaagatact aataatagat gtttaatata
      661 tgaagttaga ccattaaatt gtaggatgtt tggacattgg aaaaaagagg attataataa
      721 aaatttagat aatgttattg agaaaaataa taactataga gagttaatga aaaagcaata
      781 tggatttgaa ataaatgatg aagtagtaaa ttacaggata gattattgtg atagatttat
      841 tccaaataaa gattatttaa gtaaatctaa gagattaagc ttttttgatg agcttatgat
      901 attagactca aatatttatt caagtggaaa tatagatata gactttaggg atagaggtat
      961 agttgaatat tttatagagt cattacttta tagaaattta gcatataatg tgaaaataag
     1021 gatatctaaa gaatacaaaa taagaaaaag aactatagat agaataaaaa ggattgttct
     1081 tgtatagttt tatattagta agtaataaat tgtatcataa atattgtcaa aatgaggtga
     1141 agaaggtgaa tcttacaaaa gtaaaaggaa ataccttttt tataaaaggt ggcacgaata
     1201 caggaatata tgtatttgaa gataacacag cacttatgat agacccaggg ttatgtggtt
     1261 caagacctag gagagtaaat aaagtattag atgaaaataa tatacaggtt agatatatta
     1321 taaatactca tggtcatgat gaccactatg gagcttgtaa tcaatttaaa gaatattata
     1381 aagatgtgtg tataatgtca tctgaatacg ataaattata tatagaaaat ccagaattgt
     1441 tttcaaagta tattgttggg ggaaagagca atgtatttat ggataatatc ttaaaaaata
     1501 aaatgttgga tgatataaaa atagataaat ctatagaagc tggaaaaatt gaattaaaca
     1561 atgaaatgtt tgatataatt gaatttcagg gtcatacacc tggtagtata gctgttttga
     1621 cgaaggataa tgtgattttt gttggcgatt tattgatagg agatgaaatt ttatcaaaat
     1681 acgatttctt gtttctatat gatatacaag aacaaataaa ttcattagaa aaattaaaaa
     1741 atatagattt tgagtactta gttttagggc atggcaagca agttatatcg aaagaagatt
     1801 cttataaatt aatagaaaaa catttgaatg ctttaaaaaa atatttatat caaataagag
     1861 agttgcttgt agaaccgaaa tctttagaaa ttttattaaa aaacattata aataacaata
     1921 atttaagcaa taattataaa gagtatcatt tttttaaatc ttcgttagta tctgtcataa
     1981 gttacttagt tgatttagaa gaaatagggt atatattgga aaatggagaa ttgctttact
     2041 atacaaaaac aaaataaatt atgataaaat aaagaaggtc ataaaaaaca aataaagggt
     2101 gagcaatatg gaatacgaaa aatggaatga gatactagca ccttatgaac atgcagtaga
     2161 ggaattgaaa ataaaattta aaaatataag aaaagaatat ttagcaaaag gtgaatattc
     2221 tcccatagag tttgtcacag gtagaacaaa aaaaatatct tccataatat ctaagttaaa
     2281 aaggataaat gcaaaagata tagaaactga aattgatgat attgctggta taagaataat
     2341 gtgccagttt gttgaggata tatatgctat agttgatttg ataaaagtaa gaaatgatat
     2401 gactataata ggagaaaaag attatattac aaattacaaa gatagtggtt atagaagcta
     2461 tcatgttata ataaaatacc ctataaactc cattgcgggt tcaaaggaaa ttatatgtga
     2521 aatacaaata agaactcttg caatgaattt ctgggctaca atagaacatt cattaaaata
     2581 taaatatgac cattacatac ctgagactct tgctgaaagg cttagaagag catctgatgc
     2641 tgcctttctt ttagaccaag aaatgagtga aataagagag gacataatga aagctcaggc
     2701 tatgtaccaa atgaagtcta tagctattag agatactcta aatagaatac aagagttata
     2761 tgacttgggt gatacacata aagctatgca gtaccaaaga aaacttgata gaactgaaac
     2821 agataaaaat ataactgaaa ttttggaatt aaaacaagaa atagatatat tactagagca
     2881 atataaggat actaaggtag atgataaagt agttcagtaa aaactacttg tagaaaattt
     2941 gctaaatatt aatggggctg cctcttaatg gaaaataatt tctaaaaaga gacagcctat
     3001 ttagtgtaat taagaaagga atgatattat gagtttgtat gctataggtg atttacattt
     3061 ttcaacatca gtcaataagc caatgaatat ctttggaagt aattgggatg gtcatgaaaa
     3121 gaaaattata gataattgga aagaagttgt taaggaagaa gatatggtct tagtactagg
     3181 agatacatct tggggaatta atttatccga agctaaaaaa gatttagata taataagcaa
     3241 acttccaggt cagaaaatac ttataaaagg aaatcatgat tactggtgga caaccgttac
     3301 aagtttaaat aagctatatg aagacatgcg ttttattcag acaaattttt atgaatataa
     3361 agattatgca atttgtggag gtaggggctg gatatgtcct aatgatgtta aatttgatga
     3421 gactgatgaa aaagtttata aaagagaaga acatagactt agactatcat tagaatctgc
     3481 tcgtaaaagt ggtcactcaa aaataattgt gattactcat tatcctccta cgaatgataa
     3541 attggaagaa tctttattta caaagttatt tgaagaatat aatgttgaaa aggtgattta
     3601 tggtcacttg catggtaaag aatcttttaa aatgggattg aaggggattc gaaatggagt
     3661 agaatataca ttagcatctt gtgactatac agaatttaat ttaataaaag ttcacgatta
     3721 aaaaacataa attggtaaat actataagat acatgattaa aaataaatat ttgagggatt
     3781 tcggttttga gaaaggagac acgacttatg aggtggatat tagtttttat atttttatat
     3841 cttattcctt tagtagtgat attcagaaat tacaagaatt ttaagaggtc ttgtatctac
     3901 agtagtattt atgtggtatt agctacaaca ataatgataa gtaatattta tatgagtgga
     3961 cttaataaaa taaaagagtc tatgtattat cacaattata tttctgatga gacatatgat
     4021 gaaaagtatg tatcaaattt taaggaaaaa gaaagtactg atttaaaaca ggataataaa
     4081 aagaatgaag aaatatcaaa taataaaaat ttaaacaaca aacaagatag aaatatacaa
     4141 aataaagaaa gtaatgaaga aggaagtaat gatataaaag aaaagaataa ttctgatagt
     4201 aatgtttcag tgaataataa atcagaaaaa caagaagata atacaaaaga aaataataaa
     4261 tatacaagta aagaaaaaaa tagtgaagat ataaaaaaaa tagatatgga aagtatagga
     4321 atttttaaga aagaaattta taatatagaa agagcagctt tagttccaat gagagaatgt
     4381 ataccttaca ctaaggatat agcaaaaaat ataactaaat tatctagtat aaaaaaagat
     4441 gttacaaatg ccagaaacaa gtgtaaagaa gttgtaaaag aatatgaaaa tatgcagatt
     4501 ccagaacttt ctaaggagaa gtatacagaa acattgagta atgctagaga tgacgtaaag
     4561 aaagcatatg agcttagaga aaaatctatg gaaagtgctt tgaaattaat aaatactaaa
     4621 agccctaaat atatagggaa gataacagaa tatcttgatt tatcagataa gcagatagaa
     4681 agttttaaaa atagattgaa cgatttaaaa gaagaaataa ataattcaga aacaaataat
     4741 attaattaat aagatttaaa gatataagtt aaaagggaga ttatgatgaa taaacagagt
     4801 ataggaaaac taaatatgtt ttttatgggg attactcaaa gaaaccttga taatagggat
     4861 ttttttattg agttagaggt gatttttaaa tctggaagaa aagagtttaa aggaattgca
     4921 atagaggaag ataataaata taaatttaat tttaaaggaa aatcagattt atatacgttt
     4981 aatgagttgc ttaagaatat ttctaaagag gcagaaaatt atgatggatt agtatttaaa
     5041 tatgttgagc gaggaactat tgttgtaatt gaaggagaca ataagaaggt aaatgtaaaa
     5101 tatttagata ataaggaaga agtacctaaa atagatgaat ttacggcatc tcaaataaaa
     5161 aacagagatt attatgtaaa agtaggacaa gcaaatgcac ttttaaaaga aattggagta
     5221 cttacaaaag atggtaagat aaaaaatgat aaaataagaa aatacaatca gatagaccat
     5281 tttgttgaat tgatagatag tattttaaaa gaaataaagg ataaagactg tataactatc
     5341 ttggattgtg cttgtggaaa atcttactta agctttgtct taaattttta tataaaagaa
     5401 gtattgaaga aaaactgtta ttttatagga atagattatt cagatgtagt tatagaagca
     5461 tcaaaaaata tggctaaaaa cttgggttat aaaaatatgt catttataaa agaggatttg
     5521 actaattata cacctaatag ggatgttgac cttgtaataa gtctacatgc ttgtgataca
     5581 gctacagata tggcaatagg tctaggaatt agagcaaaat ctgaagctat agtagtagta
     5641 ccatgttgtc ataaggagtt attagggcaa tatagatatg aagctatgga accaatatta
     5701 aaacatggtg tatttaaagc tagatttgca gatttaataa ctgatggttt aagaacattg
     5761 ttattggagg gcaatggata tgatacttct gtagtagaat acatatctcc acttgataca
     5821 ccaaaaaatt taatgataag agcgataaaa actaaaacga ataatgacaa agcattaaaa
     5881 gaatataaag aactgaaatc acaatttgga gtggaaccaa ccttagaaaa acttatatat
     5941 tagttaaaaa tgtaaaattt acgaaaagct tgcttaaata tgcaagcttt ttggtattat
     6001 ataggtatag tcatatccag agttttgatt tataattaaa gctgtgctat aatatttgat
     6061 taatcaaatc aataaatgtt acatggaggt aaaaatgaac aaattgaata tatgtattga
     6121 tatagatgga acaataacaa gcccttatca ttttcttcca tacctaaatg atatctttaa
     6181 taaaaatata actgaagcag agtgtataac acataattgg gaagaattgt atggttcggg
     6241 aataagagat gtgtatgcgg agtttaataa taagtatata cactcatatg atgaagcaaa
     6301 gattgtagag ggtgctacag aggtaattag cacattatct aaaggacata atttatcttt
     6361 tgtaacggct agacatgagt gtttaactga tgtaactaaa aattggctat cgagacaagg
     6421 attttcagat atagatgttt acttattagg aagtgattat aaagttgaaa aggcaagaga
     6481 actgagttgt gacatattta tagaagataa tcctttaaac tcagtgcaac tagctgatga
     6541 tggtgttaaa gtaatacttc tggatactaa ctataataaa gatgttaaac atcaaaatat
     6601 agttagagtt agtaattgga tagatattga aaagattata aaacaaggta tttaaagatt
     6661 tgtagattga aagggggagc atatgataga tttaaaaatt gaaaatagaa aaggaaaaat
     6721 ccatgtaaag agcagtgaaa taaaagacat tttagaatta cgtccagact tcgaatatgt
     6781 acaagatata tcaaatacta taaatcaaga aaatatattg gtatttgatt gccagttgac
     6841 aggaaattct tttgatatgg atgatttaga tatagaagaa atattggaag agttaggtga
     6901 agaaatagat gagtcttatt tcagtgtttt atttgaagat gtaagagcgt atttaaaaga
     6961 tgctactgat gagatagaag cagacttaca ggataattac ttatttgata atttgagatg
     7021 ttattttgat atatacaata taaatcaaga atttacagat tttaagtttg tatttttagt
     7081 ttcttttaaa gatataaaaa tttcttcatt agcaaaccta gctaagattg tttctaagag
     7141 acagcttata ggtgcatcta agttttatag ttaaaatgaa atattattat aataaaagct
     7201 agtaaccttg taaaaatata ggttactagt tttttatttg ttcattttca caaaatttca
     7261 gaaaactatt tacattctaa aaaaagtaat gtatattaaa cataatgtac attataatgg
     7321 ataaaaagtt tctattaaat tactaagttt atttaataat caaaatcatt tatcttatat
     7381 tagtatgtat tagggggtat ttatgcaaag tttaaatgaa ataattgctg aaaatttaaa
     7441 aaagataagg aaagaaaaac atcttagttt agacaagatt gcacaactta gtggtgtaag
     7501 taagagtatg ctatctcaaa ttgaaaagtg tgaggtaaat cctactatat ctactttgaa
     7561 gaaaataact aatgggctaa aaatctcatt tacatcattg atggagaggc aggaaagtga
     7621 catagaattg attcaaaaaa gtgatataga ccattttatt gaagataatg gaaagtatat
     7681 aagttatcca atatttccat ttgactccaa aagaagattt gagatattta tgattgaaat
     7741 agaagaaggt ggaaatttag attcaaatgc tgaacttcca ggaacacaag agtttataac
     7801 tgtttttagt ggcgaagtaa ccattaaaat aaatggagaa gattacattg tatctagtgg
     7861 aaattctata agatttaaag ctgatgtatc acatatatat agaaactcag gcaaaggtat
     7921 agctaaaatg agtatggtag tatattatat ataaataatt taatggaatt agagtgatat
     7981 ttagatgatt ttaggtatga agatatatta tatttaagtt ataggagtgt gatttatgga
     8041 tgaataataa aaaagcagtt tttttctttt gcagtgattt aaagaaagac ccagtagcca
     8101 gtaatgtgat aaaatgttgt gaagaaataa tggatttaaa tcaaactgat attgttattg
     8161 atgataattt tgtattagag tttaaggata aaaacaataa cttatttcat tttgtaaaaa
     8221 caaaagatgt aataagtcat aattataaat attatcttcc aattttaaat agacatttta
     8281 gagattatga ttttgcagga gttgtaaatt ggcatgaggg agaaaatgct ccagaacata
     8341 tcctaacagc tcatacaatt ggagatgttc caacaggaga atttggaaat tcaaatccaa
     8401 gatactttaa aaatctaatc aatgctattg aggatataaa aaatgaaaat ctacttgatg
     8461 aatttactac attaacagaa gcaactcact ggtctggaac aacatatggt gaagaatcta
     8521 aattaattac agaatattct gtacctatgt tggatattga gataggaagt agtagtgata
     8581 gttttaataa tagtatagca atacaggtat tggcaaaatc attaattaga gtatttgatt
     8641 gtgatgaacc tttgaagact ttattatgtg ttggaggggt acattttgag aaatctttct
     8701 cagatattat aaaaaataaa gaatataaca tatcaattgg acatgtattg ccaaatcaat
     8761 ggattgtaag tggaatgtat gatgatgaat cgggatttga gaaactagaa aagtgtataa
     8821 atagtattga aggaggaatt gattgtatag tttttcatga taaattaaaa ggtacctata
     8881 aagagcaatg ccgtaaacta ggtgaaaaac ttaatgtacc tgtatttaaa cataaaatat
     8941 taaaaaatcc aaaagactta cctatatggt agttttatac agttaatata acaataagtc
     9001 ggattaattt ttaagcgagg tcaaaaatga tattgtctta ttttgcatta ttaattagag
     9061 tagtgctatt agcttttgaa agagtagttg taagattatt aggtgatgaa gaaggagata
     9121 tttataaaaa tatagcttca tcatttctat ttttctttat agggggagta tgtttacttc
     9181 catttagcat aatggataga gttagtgatt ggtcgttttt aataccatgt tatataagta
     9241 gtttggtcta ttcaattggt tcagtagcgt atgtaacgtc tttagcaact ggggaagttt
     9301 cacttgttac tccaataaat agtttaaatt cattgttttt actgctttta tcagttatat
     9361 ttttaggtga aggtttaagt ttagcaaaag ttgcaggaat cataattatg attggtggag
     9421 tgtttatatt aaaaaaagta agctcttttt taaagaatgt ctctgtaatt tttaacaatt
     9481 taccatgtag attaatgttt ttatacatat ttttgcagtc agtaggaaga gtattggata
     9541 aggctttttt tgtacaagta tctcctatat tatattcaac tatactgtat tttttcgtag
     9601 gtttaaattt atttatcttt ttagcattta aaaagaaaca aaaaataatt tgtgagattt
     9661 ttactaataa aaaggggctt tcaattttaa gtggtgctat taatggatat tcttatttag
     9721 cattattaat tgctttaaat aatctagaac ttagtatagc agagccattt tctcaagtat
     9781 ctatgataat aactttaata cttgctcatt ttatattcaa agaaaatata aaagaaaaaa
     9841 taccaggttc aattttgata ctaattggtg ggtggctact gttactttaa ttgatataaa
     9901 aaatgaatta taaaggtgct attagttagt atcagaaaat ttaaaaaagt attgacaatt
     9961 cttttaggca aatgttaata taataataca aaatagtata aatagaagac atgttactgg
    10021 taatgcaggc aggatcgaat atattgcttt gatttttcaa ggtgtttata taggttctgc
    10081 cttttttgca tcttgaatgg ccattcataa aaaattatgg agaaggagat tacaagatga
    10141 attataaaaa aactgccatt gacattattg aacaagttgg tggttcaaaa aatattttag
    10201 ctttagaaca ctgttcaact agactacgct ttaagttgat tgatagaagt aaagtcaatc
    10261 aggatgcttt aaaaaaagta caaggtgtta tgagtatagt taatggggca caatttcaag
    10321 ttgtcattgg aaatagtgtt gttgatgtgt atgatgaaat tttaaaagtt tgccctttag
    10381 atacaaatga tagtactgta aaaactgaag aaaaaataaa cttaggaaca agatttatag
    10441 aatttattat taatattttt caaccactga tttttgctat tgcaggagct ggtattttaa
    10501 aatcactgtt gatgttatta gcaatgttta atattttaaa atcagattct actatatata
    10561 cattattagt tgctatttca gatgcaacat tctatttcct acctcttatg gtagctgtaa
    10621 ctactgccaa tgtattgaaa tgtaatcgtt tagtagctat tgccgctgta ggatacttat
    10681 tgcttccagc aacaacaact gctttaagtg aaggtgtcga actatttgga tttacaattc
    10741 caaacattgc ctataatgca caggttttcc cggctatctt atgtgtgtct ttcttggcaa
    10801 caatggagaa aatttttaac aagtattcac caaaaccaat tcgaactttc tttgttccta
    10861 tgatgtcttt ggcaattact gttccaatta ctctaactat tttaggtcct ttaggatata
    10921 acgttggtac aatttttaca accattattt tattcttata taacaaaatg ggattcttag
    10981 ttgtaggatt acttgctgct tttcttccat ttatggttgc aacaggaatg cacaaagcac
    11041 taatacctta ttcaattaat acaattggaa agttgggtta tgaagcttta tatatgacat
    11101 cttcattagc ccacaatatt tctgaaagtg gtgcttgttt tgcagtagcc ttgcgaacaa
    11161 aagatgaaac attaaaacaa acagcattat ctgcaggaat ttctgccttg atgggaatta
    11221 cagagccagc tttatatggt attactttac aacataaaag agcaatgtta ggtgttgtat
    11281 tctcaagtgc aatttctggt acatttttag gattatttgc agtaaaagga tttgtaattg
    11341 ttggaccagg gcttgccagt atgagtatgt ttattgaccc aaacaatgga aacaatttga
    11401 tttttgcaag tattggtttt gttattgcaa tattaggctc gtttattatt acactgatta
    11461 tttggaagga agatacaaat acagtagagt catcaattgg agatgttgtt gaagaagagc
    11521 tagaagaaga aaaagaagta aatatcttag taactccagt agaaggaaaa gtaatagatt
    11581 taagtaaggt taatgatgaa ctatttgcaa gtaagacttt gggagatgga gttgcaatta
    11641 taccgaccaa tggaaattta tacgcacctt gtgattcaga agttgtgatg ttatttgaaa
    11701 caaaacatgc aattggttta agaacaaaaa atggggctga gattttgatt catattggaa
    11761 taaatactgt aagtatgaat ggagatggat ttaaaacatt tgttaagaca ggcgataatg
    11821 taaaagaagg agatttatta attcaatttg atttagacaa aatctcacat gctaatttag
    11881 attcaactgt tatgattgta aacaataatg gagcagatta tgcttataaa gtgttaaacc
    11941 agtcttatgg caatgtaaaa aaaggaagta tcttgtttga tgtaaaggga ggaatataaa
    12001 tatgagaaag tttccagaag gatttctttg gggaggagct acagctgcta atcaatttga
    12061 aggtggttgg aatttaggtg ggaaagggtg gtctgtatca gatgtagcaa aggcacactt
    12121 tgatgcagat gtgaaagatt ataaatctaa taatgaaatc acaacaaaag atatagaaga
    12181 aggattagca catcctgaag atgaagtaaa ttatccaaaa cgtcgtggta gtgattttta
    12241 tcatcactat aaagaagata ttgctctaat ggcagaaatg ggatttaaga catatcgaat
    12301 gagtattgct tggtctcgta tttttccaaa tggagatgat aaagaaccaa atgaagaagg
    12361 tttacagttt tatgatgatg tatttgatga gttgataagt tatggaattg aaccacttgt
    12421 tactatgtca cattatgaac caccattaaa tatcgtttta aactatgatg gatggtattc
    12481 acgacaagtt atcaatatgt ttgttaggta tgtagaaact atctgtgaaa gatataaaaa
    12541 caaagtgaaa tattggctta catttaatga agttgattct atgattcgtc atccgtacac
    12601 tactggtggt ttagttcgag accgttttaa ggataaaaac tttgaggaag ttatttttca
    12661 agctatgcat catcaatttg tagctagtgc ccttgcaaca aaaatttgtc atgaaattat
    12721 tccaaattca aaagtagggt gtatgttaac aaaattaacg tattatccat atacatgtag
    12781 accagaagat gttttagcaa ctcaacaaaa aatgagaagt atttatgcat atagtgatac
    12841 gcaggttttt ggtgagtatc cagtatattt actgtcgtat tttaaaaata ataacattca
    12901 aatagtaaaa gaagaacatg atgatgaaat tatgaaaaag tatccagtag attttatttc
    12961 attctcttac tatatgtctt cttgtgaggc agctgataca actggattag atattacacc
    13021 tgggaatacg ttattagctg taaaaaatcc ttatttagaa atgagtgaat ggggttggca
    13081 aattgattca ataggtctac gtatatcttt gattgaatta tatgatagat acagaaagcc
    13141 tttatttata gttgaaaatg gattgggagc caaagatatt ttaactgaag ataaaaaggt
    13201 tcatgaccaa tatagaatta attatttaaa agaacacttt aaatgtatgt tagatgcgat
    13261 tatcgaagat ggcgttgaac tttggggata cacttcatgg ggttgtattg atttggtcag
    13321 tgaatcaacg aaacaaatgt caaaacgtta tggatatatt tatgtggatg ctgatgatta
    13381 tggaaagggg acatataatc gatataagaa agatagtttt tattggtata aaaaggtaat
    13441 tgaaagtaat agtatagatt ttaaatgacc aatagaaggt gagcaaataa aatgaactac
    13501 ataataaaga aagttttgaa ctcgagcgtt gttttagttc atgatgcaaa gtataatgaa
    13561 tttattttgc tagggaaagg aattggatat ggtaaaaaaa caggagaata tatttgtggt
    13621 tccaatgata atcaaatgtt tgtccctgtt gaaaatacaa aatcaaaaca atttttagaa
    13681 ttaatggatg atattcctgt cgacatttta aagataacac aggaaataat agtagaggca
    13741 gaaaagttat tgaactcatc ttttaataaa aacttatact taatattagc agaccatttt
    13801 aactttgcaa ttgaaaggat gcgtaaagga ataaaaatta caaatcgagt attttgggaa
    13861 ataaaaaatt attatccaaa tgagtttaag gttggaatgg ttgcaattta tatggtagag
    13921 gagagattgg gcattcaatt accagaggaa gaagcagcta acattgcttt ccattttgca
    13981 aatgctatgg cttcagatgg aaattcatat gatacgatta agtatgccaa agtaattgga
    14041 gaaataatta atatttttat tttttctttg aatagaacct tagataaaaa gagtatgcat
    14101 tatatgagat ttataacaca tataaaattt tttgtggaaa gatttttttc agatagtatg
    14161 ttaagcagtg gtgatgattt attatttaat caaatgaaaa ggtcaaatcc aaaagaaatg
    14221 ttaattgcat taaaagttag agatttttta gaaaagaaat atggaaaaaa gttaacaaat
    14281 gaggaaatag ctttcttggt tgtccatatc gcacgagtta agcaatagag taaattacta
    14341 atattatctt atacgtctac atggtagaaa attgaaagaa ggtaaagtat ataaagaaac
    14401 tgagacatat tattttgttg ttcattatgt ttattttatg agtgtatcct gttaaaaatt
    14461 taagttaata tttataatta aaatatgcaa atagttaaaa taagtttatg tggttatgaa
    14521 tttattttaa ctattttttg tttaatggaa aatatgcttt ataactagaa ttttattttt
    14581 aattaattat aatacaaaaa actttataaa atcttcagaa tttcttaaa
//
LOCUS       DAEMHZ010000106        14447 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000106 DAEMHZ010000000
VERSION     DAEMHZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.106"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            13..456
                     /locus_tag="KPK72_002820"
     CDS             13..456
                     /locus_tag="KPK72_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490018.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            471..1055
                     /locus_tag="KPK72_002821"
     CDS             471..1055
                     /locus_tag="KPK72_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490019.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1308..2345
                     /locus_tag="KPK72_002822"
     CDS             1308..2345
                     /locus_tag="KPK72_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2490020.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2426..2791
                     /locus_tag="KPK72_002823"
     CDS             2426..2791
                     /locus_tag="KPK72_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490021.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2796..3113
                     /locus_tag="KPK72_002824"
     CDS             2796..3113
                     /locus_tag="KPK72_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490022.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3161..4765
                     /locus_tag="KPK72_002825"
     CDS             3161..4765
                     /locus_tag="KPK72_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490023.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4889..6517
                     /locus_tag="KPK72_002826"
     CDS             4889..6517
                     /locus_tag="KPK72_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2490024.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6782..8131
                     /locus_tag="KPK72_002827"
     CDS             6782..8131
                     /locus_tag="KPK72_002827"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG2490025.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNSDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8287..9366
                     /locus_tag="KPK72_002828"
     CDS             8287..9366
                     /locus_tag="KPK72_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2490026.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9711..10619
                     /locus_tag="KPK72_002829"
     CDS             9711..10619
                     /locus_tag="KPK72_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2490027.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10624..12993
                     /locus_tag="KPK72_002830"
     CDS             10624..12993
                     /locus_tag="KPK72_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG2490028.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13318..14121
                     /gene="proC"
                     /locus_tag="KPK72_002831"
     CDS             13318..14121
                     /gene="proC"
                     /locus_tag="KPK72_002831"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2490029.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5921 a         1469 c         2647 g         4410 t
ORIGIN      
        1 atgattattt aagtgaaagt ggataaaaca ataaattttc ttaatggatt taaaaaaact
       61 aaaccatgta ataaaatcta taaaataata atagtcttaa ttttaacgtg tattttattt
      121 aaatcattta tgtatgtgaa atctataaat gaacttgaag attatataag tcaaaataag
      181 ctagtggata aatcaaagaa cactttacca acaacagata aaactagaga gaaattttct
      241 aaaataaaac ttaatgaaat aaaaaaaata tatgaaacaa ttggaataaa tagtattact
      301 aaaatatcaa tagataaaaa taagctagag atggaaggtg tgtgtagtga tttagatgtt
      361 ttaaacaatt taaggaaaat aaagtacata aaatattatt acatagacag tattgtaaga
      421 gatggagaat attatagatt tagaataaaa agttagatgg gtgattattt ttgagattaa
      481 ataatagaga aaaaaatcta cttaaaatat tgttaatatt agttattata ttggcaagtg
      541 atatagcagt aagttatttg gaagaaaacc taactagttt gaagaatgaa aagcaaaata
      601 taataaataa aataaataat caagataatt atcaaataaa ggataataag catatagatg
      661 ataaatatat agaaagtgtt gaattaaata aaaatgaaga attgaaaaat aattttaata
      721 actcattttt agataaaaaa acaaataaag atatagtgtt attagtagaa aatgaactga
      781 gagatattgt gaatgtagaa tatattaatc aagaaagtag tttagataat cacaataaaa
      841 aacaagttaa tttagaaata aaagttaatg gagaacttgc aaatgtgttg aaacttgaag
      901 gtgtaatgaa gaagttaaga ctaaataata aacttgaaaa gatagaaatt aataaattaa
      961 aagtcaataa ttttgatgtt caaaatattc caaatgaata taaacaagaa aaagacaata
     1021 ttgttgaatg taaaatgaca gttagagtca tataacaaaa aagaatagat aaagtaaaaa
     1081 actattatca attaataaaa ttttatagag gaattagttt tacgataatt catttctaca
     1141 agagggattg ttgatgattt tatcgaaatc ctaattaaag ttaatatatt gtaccaaaat
     1201 ataagtgaat ttaatagaat atttacttat actatagatt gattaataaa aaaatggtat
     1261 aataacaaat tgtactttaa tttaataata agggagtgga agtcatattg ttagggagaa
     1321 atgagctatg cccttgtgga agtggcaaaa aatacaagaa atgttgttta aataaagata
     1381 ttgtttcaga aagagcatgc agaaaaactg aactttcaca aaaacaatat acagatttat
     1441 attcaaagtt atatgattat tcaagacagg ataagtttaa agaagaatgt gaaaaagcta
     1501 aagaaatgtt ttatataatg caaaatgata gtgtaaatga aaaatttgaa agatttttta
     1561 atacttattt aatacaagac cacataatgg aaaataaaaa ggttatgaca gttggttttc
     1621 ttgaagaaaa tggtgcaaac ctaagtcaat cagaagtatc aatattaaaa agtatgtttg
     1681 aatcatacgt aagtatttat gaggtaaaag aagtttctgc tggaaaaata gtattaaagg
     1741 actgtttaag tggaaatgaa ctttgtacag aagatgtaaa acttctgaga agttttaaaa
     1801 tcggaagttg tatgatagct agaatagttg atattgaagg aactaacata ttgatagata
     1861 taactataag tatatcaaat gaggttaaag atataatttt aaaggatatt atgaatctat
     1921 tcaatcaata tcaagatgta tacaaagata tgaagtcgtt cttaatttat catacacata
     1981 tactatacaa gtatatacaa cagcttttag acccaagtat agctgaatat ttaaagagag
     2041 aaagagataa aaacgataca aaagttaatt cagaaaatga attagctgaa gaagaatgta
     2101 aagttttaga tactcttaaa gaaaatgtag aaaaagaaaa tatagatgca tgtatagagt
     2161 tttggaatga atataagaaa aatcatgaaa atataaaagg agctgaaaat ggttgggcag
     2221 ctgctgttga atactatgtt aaaaaagaag caggacaacc tataactcaa gttcaaatat
     2281 caaagaaata cgatataagc tctagtacac taggaaagag atacaaggat ttaaaaatat
     2341 cttaattaat tataaaaagc tttaagtcat agataaagaa tctatagtat aatatatgta
     2401 taatgtttag aaagaggtat taataatgag tcttagttta ggaaatgcaa ttgctgtatc
     2461 aacgaacttt ataaaatcta acccagctgt acctgtgtac aacggagaag aaatatatgc
     2521 agttatttat catatagaaa taggatataa taatggtgta tcacctgata ccaaacaata
     2581 tataataata gactataaag agtttgattt atctatggat aaaaaaaatg aactaataaa
     2641 tattgctaaa gagtactgta aatctaaagg tattgtagaa aacgaaaacc ttgaaataaa
     2701 ccttttagac aatgaagaag cagagaactt cttagaaaag gtgtttaata atatgaaggt
     2761 tataagaggt cttataacta aaaaaggata aataaatgga ctatatagga atagaaaata
     2821 taacacctta tgaaaataca tatgaattta gtgtatatga atatgatgat gaaatcacct
     2881 taggtagtga aaagttatat gtatgtgaat tgagggttgt attgattaaa gttaattctc
     2941 tgtatgttga aagattgcat aaatcagttg aagcaatggt cttagtaaaa aacttgaaaa
     3001 aagatttaga taaaacactt gttgtaaaca aaataaagaa ttttgtgcta gatgagattt
     3061 gggtagaaaa tctagtaaaa gagaatatag aagttatatt tgtagaaagc taggaataac
     3121 cctagctttt ttatattaca atctaagaga ggaaaacaat atgttaagag tatctaatgt
     3181 aaaacttgga attgataaag atatatcttt attaaaagat gttatactta agaagctaaa
     3241 aataaaagaa aaaaacttag tcaaatatag tatatataaa gaatctatag atgctagaaa
     3301 aaaaggaaaa atggaatttg tttattctgt agatgtagaa gttaaagatg aaaataaaat
     3361 acttaactca aaaataaaag atgtaacaaa aataaaggaa atcagatacg taaatgtacc
     3421 tgtgggtgat aaaaaattaa agaataatcc tttggtaatt ggtagtggac cagctggttt
     3481 gtttgcaggt ctgcttttag ctcaaatggg ttatgaacca atcatacttg aaagaggtct
     3541 tgatgtagac agtagaacca aagatataag tgatttttgg acaactagaa aattaaaaaa
     3601 taattcaaac gttcaatttg gagaaggtgg agcaggaact ttttcggatg gtaaacttac
     3661 aactagaata aaagatatac gatgtagaaa agtattagaa gagttagttc agtttggagc
     3721 accagatgaa atattatact cacataagcc acatgttgga acagatatat taaaaaatgt
     3781 agttaaaaat ataagaaatg aaataataag tttaggtggt caagttagat ttaattcaaa
     3841 agtaacagat atagttgtat caaatgattc tatacaatct gtaataataa atgatactga
     3901 aaaaatagat acagatacaa ttatattagc tgttggacat agtgcaagag atacttatga
     3961 aatgttgtat tcaagaggta taaaaatagt tcaaaaacct tttgcaatag gagctagagt
     4021 tgaacatcca caagatttaa taaataaagc acagtacaag gatttttata atcatcctag
     4081 gttaggagct gcagattata gacttataga gcatgtttca aatggaagaa cagcatatac
     4141 tttttgtatg tgtccaggag gtagtgtcat agcatctgca tcaaatgatt ttgaaatagt
     4201 tacaaatggt atgagtgaac atgcaagaga taaaataaat gcaaatagtg catttttagt
     4261 aaatgtaacg ccagaagatt ttaagagtga taatccacta gcaggtgttc atttccagca
     4321 gaagtatgaa aaattagctt ttgaattagg tggaaaaaat tataatgcac cagttcagct
     4381 tgttggagat tttataaaca ataagacttc tttgaattta ggaagtgttg agccatcata
     4441 tagaccagga tatacactta caaatttaag tgaatgtttg cctagcttcg taacagagac
     4501 tatgagagaa gcgtttatat ctcttgataa aaaattgaat gggtttgcaa tgtatgatgc
     4561 tgttttgacg ggagtagaaa ctaggtcatc atcaccaata agaatagtaa gagatgaaaa
     4621 tactttagaa tcagtgagta ttaagaatct atatccatgt ggtgaaggtg ctggatatgc
     4681 aggtggtata gttacagcag cagttgatgg tataaaatgt gcagaaaaaa taatacaaaa
     4741 atacaccaat aatacaaaaa tttaacataa acttaatgat tttttaatat ttaacatgaa
     4801 tttaacatta tcactcctat aatttgataa aatacaatag gttataaaat ttttagatac
     4861 agtaatctct ttacaaggag gaaataattt gaacattata ataagtctaa tgggtggatt
     4921 aggtctgttc ttatatggta tgaaccttat gggagaaggt cttcaaaaat ctgctgggac
     4981 aaagctaaaa aagataataa aactacttac tagtaaccta tttatgggag ttttggtagg
     5041 tacaggagtt acagcagtaa tacaaagttc aagtgctact actgtaatgg ttgttggttt
     5101 cgtaaatgca ggaataatga ctttaaaaca ggcaatagga gttataatgg gtgctaatat
     5161 agggactact gttacagccc aattggtatc tttcgactta actggtatgg ccccagtagc
     5221 attaggtgta ggtattatac tttatttatt tggaaataag cctagaataa aaaatatagc
     5281 cgaaatactt ataggttttg gtatattatt tacaggtatg gattttatga agatggcagt
     5341 ggagccacta agagattatc aaggatttac tgacttactt gttacatttg gtagatatcc
     5401 actacttgga ttattattgg gatttgggat tacagcaata atacaaagtt caagtgcatc
     5461 tatgggtatg ttggttgcac tggcagcaga aggtctagtg ccattatcag cagcactgcc
     5521 aattctttat ggccaaaata ttggtacatg tgtaacttca ctgttatcaa gtattggtgc
     5581 taacaaaaat gcacgaagag cagctatgat gcatctaatc ttcaatgttt taggaacggt
     5641 gatattctta atattcttga ataaaccagt agtaagcatg gttacaagtt gggacccaag
     5701 caatgttgca agacaaatag ccaatacaca tacattgttt aatataatat ctgtattgat
     5761 acttttacca tttacaagtc ttattataaa actagctata aagcttgttc ctgatagagc
     5821 aggtgatata gatgaagatg aaactaaaac tattaaatat atagatgata gaatgataga
     5881 aactccttct atagctcttg caaatacgat aaaagaagca cttcgtatgg gtgagaaggc
     5941 gaaggaaagc ctaaatgcat ctatggaagc tttagtagaa cattcaactg aaaaaataga
     6001 taagacatat aggcgagaga gattaatcaa tgatttacag aaagctattt taaattattt
     6061 acttaaactt tcaaaagcac ctttagatga tgattcaaga gaagttgtgg atactttgtt
     6121 taatacagtc aatgatatag aaagaattgg tgaccacgct aaaaatatag ctgaattatc
     6181 gcaagttgca atagattcaa atatttcttt ctcagaagaa ggacaatctg aacttgatgt
     6241 tatgtataat agagttgttt cagcatatac ttatgcatta gaatcaatga gaactgataa
     6301 tgttgattta gcttgtaagg ttataaaaat tgaagaacaa gttgatataa tggagaaatc
     6361 ttgtagagct aatcacatgt atagattgaa caataatcta tgtagtatag aaaatggagt
     6421 tatttattta gacgtaataa gtaatttaga gagaatatct gaccatgctg tcaatatagc
     6481 acaacaagtt atagcgaaga gattaggaaa tgattaaaaa taattaattt aaaataatat
     6541 aaaatttaaa atagaggata aataaaagag aaactttaaa tgatacgttt agagtttctt
     6601 tttttgtaaa tttatttgac taaaattttt aaatttatac atagttatag tatttagaag
     6661 aatcgatttc ctgataataa atgatgagat atgtatgatt ttgtggcaaa cttttaaaaa
     6721 aaacacctat attttgtgtt ataatctctt taaaacgaat atgctgaaaa gagggattta
     6781 aatgggaaaa ataaattttg attatagtaa agctacagag tttttttgtc aaaacgaaat
     6841 agatgtcatg caaccatatg tagatgttgc gcacgacatg ttacataata aaactggttt
     6901 aggaaatact tttttaggat ggatagacct tcctaaaaat tatgataaag aagagtttga
     6961 cagaataaaa aaatctgctg ataagataaa atcagactca gatgttcttt tggtaatagg
     7021 tataggaggc tcttatttag gttcgagagc tgctatagat atggtaagtc attcttttag
     7081 gaatggactt aaaaaagagc aaagaaaggc tccagaagta tattttgtag ggcataatat
     7141 aagctcaaca tatattatgg atttattaga tataattgaa ggaaaggata tttctgtaaa
     7201 tgtaatatct aaatcaggaa ctactacaga accagctctg gcttttagaa tatttaaaga
     7261 ttacttagag aaaaagtatg gtaaggaaga agcaagaaag agaatttatg caacaacaga
     7321 tgcaagtaaa ggtgcactta gacagttagc tacagaagaa ggatatgaga cttttgtaat
     7381 accagatgat gtaggtggtc gtttttctgt attgacagca gttgggttac ttcctattgc
     7441 tgctgctgga cttgatattg atgctatgat gaaaggcgca aatgatgcta gagaagcatt
     7501 tcaaaattct gacttaaaaa gtaatgatag ttatagatat gctgttgcta gaacaatttt
     7561 acatagaaaa ggaaaagatg tagagttatt agtgaactat gaacctcaac tacattatgt
     7621 aagtgagtgg tggaaacagc tttatggaga aagtgaagga aaagaaaata aaggtttatt
     7681 ccctgcttct gtagatttct caacagattt acattctatg ggtcaataca tacaagatgg
     7741 aaaaaggctt ttatttgaga cagtacttaa tgttgaaaac tgtaagagaa atataactat
     7801 aagttcagag gaagtagact tagatggttt aaactattta gcaggtaaga ctgtagattt
     7861 tgtaaatcat aaagcttttg aaggaactct attggctcat acagatggaa aagtacctaa
     7921 tttagtaata aatatacctc aactagatga gtataatttt ggatatttag tttatttctt
     7981 tgagaaggct tgtggaataa gtggatattt attgggagtt aatccatttg accaaccagg
     8041 agtagaagct tacaagaaaa atatgtttgc tttactaggc aaaccaggtt atgaaaaaga
     8101 aaaggaagag ctagaaaaaa gacttaaata attttaaata gttaaggttt aattaagatt
     8161 aaaataatat aatgaaatca tcaaaaagga gtttactcaa aatagatgat ttcatttttt
     8221 ttatgaaaaa ttgggaaaaa tatattttga taatttatat tatattaaga tagggagtgg
     8281 tatgagatgt caagaagaaa gaaagggata agtttagtaa tattagttgc tataataagt
     8341 tcaatactta gtagttttct aacaataatt ttagttaagg acaatttagt aagtaaatct
     8401 acaggaagtt ctacgccgat agttgtaaat gatgatggta agagtcaaaa tatttatcaa
     8461 gcagttgcag aaaaagctac gccatctgtt gtaggtatta caacaactag tgttgatact
     8521 agtaatatgt ttgcaatacc aactgaaaca caaggtgtag gaactggaat catagttgat
     8581 tcaaatggat atatacttac taattctcat gttatatcag atggtcaagc tacaagtgta
     8641 aatgtattat tcaatgatgg ttcaacaact tcaggtaaag tagtatggtt tgaccaacaa
     8701 cttgatttag ctatagttaa ggttgataaa actggactaa caccagcaga atttgctgat
     8761 agtgacaaag taaaagttgg agatatatca atagcaatag gtaatcctct tggattagat
     8821 tttcaaaaaa cagttactca agggataata agtggattag atagaactat acaaacagaa
     8881 aaaactaata tgactggttt attacaaact gatgcaagta taaatgcagg aaatagtgga
     8941 ggaccattgt taaatcaaaa aggtcaagta attggtataa acacagcaaa agcttctcaa
     9001 gcagaaggat taggatttgc aataccaatc aatacagcta agagtatagt tgaagaagta
     9061 atcaaaaatg gaaaatatga aaaagtaact ttaggcataa aaggtacaga tgtatcaaat
     9121 tatgaagcag caacaggtac aaaattaagt actgataaag gtgtttatgt tgcagaagta
     9181 atatctggtt catcagcaga aaaagctgga gtaaaggtag gggatattat aactaaagta
     9241 ggagatacag atataacagg tatgaatgat ttaaataaaa agttgtatac tttctcaaaa
     9301 ggtgctagta caaaaataac agtaaataga ggcggaaaag cagtaacaat aaatgtgaat
     9361 ttctaaatat gaccagaaag atagagacta catggtaaag tgtagtctct atttttattt
     9421 aatttttata gtatatacat ctaaattaat taaaatgatg tattgtttat agtttattgc
     9481 tatattttct tataaaaata aatttaataa atatttaaat ttaatttaca aaaacaatat
     9541 aaatatcata ataaaataaa attttttaat attactaaaa atatatagac aaaataataa
     9601 caaatatagt attatgtaat tgtagaaatg cttttcatag gtatattcta tgaagatgaa
     9661 ttgcataaaa atgaaaaaat ggaatggata ttatttaggg gtgaaaaatc atgaatccat
     9721 tagttataaa cttacaaaaa tgtagcatac atgatggacc agggataaga agtacagtat
     9781 tttttaaagg gtgtccactt gaatgtgttt ggtgtcataa tccagagagt caaacatata
     9841 caaaacaagt tttgtataat gaagaaagat gttcaaaatg tgaagcatgt ataaatatat
     9901 gccctcataa ggcaatttac aaaggtgaaa caaaaatatg cttagaccaa gataagtgtg
     9961 agttttgtga aacttgctta gactattgtg taaacaatgc aagagagatt gttggtcaag
    10021 agtattctgt aagagatttg gttaaagaga tagagaagga tagaattttc tatgaagaat
    10081 caggtggtgg agtaactcta tcaggtggag aagtaatggc tcaagatatg gacttcattt
    10141 gtggagtaat aaacatgtgc aaatcaaagg gaatccatgt agcaattgat acatgtggct
    10201 atgcaaagtc agaaaattat gaaagagttg ctaaatgtgc agatttgttt ttatatgata
    10261 ttaagcttat tgatgaagat aagcacataa aatttacagg aaaatcaaat gacttaatac
    10321 tgaaaaatgt taagatttta agtgaattag gggctaacat aaatataaga ataccattaa
    10381 ttgtaggagt aaatgtagat gatgaaaatt tagaggttaa aaaaatgata gagtttttaa
    10441 agcctttgaa tatacaagca gtaagtcttc ttccgtatca taatattggt aaacacaagt
    10501 atgataaaat ttataaaaag tatgaaggag aagaactaca aagaccatca gaagaaaaat
    10561 tagaggaaat aaaaagactg tttgaagcaa gtaatttcaa tactaagatt ggagggtaat
    10621 aaaatggcaa gaggaacttt tgagagaact aaaaaattaa gagaagaaag tattaatgca
    10681 gaaccacaca tatctattga aagagcagta cttatgacag aagcttataa aaaatatgag
    10741 ggaagtgtag agattcctgt acttcgtgct ttatcattta agcattatat tgagaataga
    10801 actcttagca taaatgatgg agaattaata gtaggtgaaa aaggtgattc tcctaatgga
    10861 gcaccaacat atccagaaat ttgttgtcat acaatggaag atttagaagt aatgcacaat
    10921 agagatataa taaattttag cgtatcagaa gaagctagaa aaatacataa agaagaaata
    10981 attccttttt ggaaaaagag acaaacaaga gataagataa taaatgcaat gacacctgag
    11041 tggttagcag catatgaagc aggtatgttt acagagttta tggaacaaag agccccagga
    11101 catacagttt gtggagatac aatatataaa aaaggatttt tagatttaaa gaaagacata
    11161 gaagcaagat taaaagagct agattttctt aatgatttag atgcatataa taaaaaagca
    11221 gatttagaag ctatggcaat agcttgtgat gcaatggtta tattaggtaa aagatatgct
    11281 gagaaagcaa gacaaatggc agaagaagaa acagatgaag ctaagaaaaa agatttatta
    11341 ctaattgctg aaacttgtga tgttgtacca gctcacaaac cagaaactta tcatcaagct
    11401 atacaaatgt attggtttgt tcatataggg gttactactg aacttaatat atgggatgct
    11461 tttactccag gaagacttga ccaacatcta aatcctttct atgaaagaga tgtagaaaat
    11521 ggaatactag atagagatag agcacaagaa ttacttgaat gtttatgggt taaatttaac
    11581 aatcagccag caccaccaaa agttggtata acattaaaag aaagtagcac atatacagat
    11641 tttgcaaata taaacactgg tggaataaat ccagatggtc aagatggtgt taatgaagtt
    11701 agttatataa tacttgatgt tatggatgaa atgaaattga tacaacctag ttcaaatgtt
    11761 caaataagta aaaagactcc tcaaaaattc ttaaagagag cttgtgaaat atcaagaaaa
    11821 ggatggggtc aaccagcatt ttataatact gaagcaatag ttcaagagct tatggaagct
    11881 ggaaaaacta tagaagatgc aagacttggt ggaaccagtg gttgtgttga aactggatgt
    11941 tttggtaaag aagcatatgt tctaactgga tatatgaaca ttccaaaaat acttgaatta
    12001 acattaaata atggatatga cccaattagt aaaaaacaaa taggtattga aactggagac
    12061 cctagaaatt tccaatctta tgaggaacta tttgaagcat ttaaaaagca attacattat
    12121 atgattgaca taaaaataga aggaaatgct gtgatagaaa atatatgtgc aaaacacatg
    12181 ccttgtccac ttatgtctac aatagttgat gattgtatag aaaaaggcaa ggattaccaa
    12241 agaggtggag caagatacaa cactagatat atacaaggtg ttggtatagg aactataaca
    12301 gatagtttaa cagcaataaa atataatgta tttgataaga aaaaattcga tatggataca
    12361 ttgcttaaag cacttgatgc taattttgaa ggatatgaag ctatacttaa tttagtttca
    12421 aataagactc ctaaatatgg aaatgatgat gattatgctg atgaaataat gcaagagata
    12481 ttcaatgcat attacaatga ggtaactggt agaccaactg tttgtggtgg agaatacaga
    12541 gtagatatgt taccaacaac ttgtcatata tactttggag aaattatggg agcaagtcca
    12601 aatggtagac tttgtgcaaa accagtttca gagggaattt cacctgaaaa aggtggagat
    12661 actaatggac ctacagcagt tataaaatct tgtgctaaga tggaccacat aaaaactggt
    12721 ggaacattat taaatcaaag atttgcacca tcagtagttc aaggagaaaa agggttagat
    12781 aatatggcta atctagtaag agcatacttt aatatggatg gtcatcatat acaatttaat
    12841 gtatttgata aaaatgtact gttagaggct caaaagaatc ctcaggatta taaagactta
    12901 atagttagag ttgcaggata tagtgaccat ttcaataatt taagtagaac actacaagat
    12961 gagataatag gaagaactga gcaaacattc taggtttgct tgtaaataaa acttcttact
    13021 atataaaatc gtaactgata taataaagta taaatggttt gattccattt ttaaaatttt
    13081 agattaatta tgagctaact ttctaaatag gaagttagct cgttaagttt aatgaaaaaa
    13141 tcgttaaatt tatatttagt aaatcataca catattagta agtaatacat acaaaagaat
    13201 aatttattat agttataata aggttatatt ttataaaaat aagataattt taagcagtta
    13261 aaataaagtc aatttagcta ctttaaaata aaactaatag aaaaaaggag atttgatatg
    13321 aaaactttag gatttattgg ttcagggaat atgggaagtg caatgatagg tggcatagta
    13381 cagtcaggat tagtaaaggc agaagatata actgtatcag atttaagcca agttgcctta
    13441 gataaagtaa aagaagaatt tggagtaaat actacaactg attcaaaggt agctgttaca
    13501 aattctgatg tggtaatagt agcacttaaa cctaatatat gcgaaaaggc tttaactcca
    13561 cttaaagaat taatagatgc aaataaaata atagtatcta tagcagcagg taaaactata
    13621 gaaagtctgg aaagttttat aggttcagat aaaaaaatag taagagtaat gcctaatact
    13681 ccagcccttg ttggcgaagg aatgtcagct ctttgtaaaa atagtaatgt aacagatgaa
    13741 gagttaaata tgattaaggc tctgtttgaa tcttttggtg aggcagaaat agtatcagaa
    13801 tatttaatgg atacagttac aggtattagt ggttcttctc ctgcatatgt atttatgttt
    13861 atagaagcta tggcagatgc agcagtatta gctggaatgc caagacaaca agcttataaa
    13921 tttgcatctc aagcagtaat ggggtcagct aagatggttc ttgaaactgg aaaacatcca
    13981 ggtgaattaa aagatatggt ttgttctcca gcaggaacta ctatagaagc tgttaaggta
    14041 ttagaagaag aaggatttag agcagcagtt ataaaatcta ttgttgcttg tatagataag
    14101 tctaaagata tgagtaaata acagatgtaa ttaagtttac tatatggttt cttgaattgt
    14161 ttttttagtg cttatttata tctatacata aatgtatttg gtataagtaa aataagagta
    14221 atttactata aaaaggaatt taatgattga gtgagaattt attacttaaa tattaataat
    14281 taaatagata tatttttaac tatatttgag atatatcatt gtgaaaaaat aaaatttata
    14341 agtattattg tgaggttata agtagattta tttaaaaata aatttattta tagccttttt
    14401 aaatttgaaa taatattcat aaggtaataa aagttacaaa tattttc
//
LOCUS       DAEMHZ010000107        14444 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000107 DAEMHZ010000000
VERSION     DAEMHZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.107"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            20..1450
                     /locus_tag="KPK72_002832"
     CDS             20..1450
                     /locus_tag="KPK72_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490030.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1650..2234
                     /locus_tag="KPK72_002833"
     CDS             1650..2234
                     /locus_tag="KPK72_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG2490031.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2338..3117)
                     /locus_tag="KPK72_002834"
     CDS             complement(2338..3117)
                     /locus_tag="KPK72_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2490032.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3175..4431)
                     /locus_tag="KPK72_002835"
     CDS             complement(3175..4431)
                     /locus_tag="KPK72_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG2490033.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4689..5588
                     /locus_tag="KPK72_002836"
     CDS             4689..5588
                     /locus_tag="KPK72_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG2490034.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5612..7060
                     /gene="ddlR"
                     /locus_tag="KPK72_002837"
     CDS             5612..7060
                     /gene="ddlR"
                     /locus_tag="KPK72_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG2490035.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKSADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7119..7925
                     /locus_tag="KPK72_002838"
     CDS             7119..7925
                     /locus_tag="KPK72_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2490036.1"
                     /translation="MNLILGDNMSKKLKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELVPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            7951..9870
                     /locus_tag="KPK72_002839"
     CDS             7951..9870
                     /locus_tag="KPK72_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2490037.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10112..10459)
                     /locus_tag="KPK72_002840"
     CDS             complement(10112..10459)
                     /locus_tag="KPK72_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG2490038.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10446..12374)
                     /locus_tag="KPK72_002841"
     CDS             complement(10446..12374)
                     /locus_tag="KPK72_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG2490039.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(12785..>14444)
                     /locus_tag="KPK72_002842"
     CDS             complement(12785..>14444)
                     /locus_tag="KPK72_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG2490040.1"
                     /translation="KIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDLEFV
                     CKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELDEVT
                     DKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLNYLL
                     EDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTISTSGS
                     TGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVGASI
                     DIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERLKGY
                     RKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPIGAP
                     GELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLDYIG
                     RMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEEDSGK
                     INERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         5361 a         2157 c         1907 g         5019 t
ORIGIN      
        1 ttaggaggaa aatataaaaa tgaatttaga tttggaattt tatcaaaaaa tattagaagc
       61 ttcccatgat gaaatatgtg tttctgatga taaaggaata ataatatatt gtaataaagc
      121 atttgaagaa aattatggtc taaaaaaaga agatatttta ggaaaaaatg tttctttttt
      181 agaagactct ggatattcaa caaaaagccc cataccaatt gtattaaaaa caaagactaa
      241 attttcactg gaacaagata ctcaaacagg gaaaaagctt atcataactg ctacccctat
      301 atttgatgaa aatggaaact tagaatttac tgttgaaaat tgtagagata taactgaact
      361 taataatata aaaaataaac ttgaagacac aaaaaagcaa gtaaaaaaat ataaatctga
      421 agtagaaaca ctatacagaa ctgcacttag aattgaagat actgtaatta tggacggaat
      481 agttatgaga ccaatcataa acacagtaaa tcatgtttct aaaacggatg taagtgtact
      541 tttattgggt gaatctggaa caggaaaaag ctcacttgct agatatatac accacaacag
      601 caatagatct aatggcccat ttataacaat aaattgtgca actatatctc cacaacttct
      661 tgaatctgaa ctttttggat atacttcggg tgcttttaca ggtgcaagta ctaaaggtaa
      721 agttgggctc gtagaattag ctaatggagg aactcttttt ttagatgaaa taggagatat
      781 accacaaaat ctacaggcaa aatttttgca attaatacag gatagaactt ttactccagt
      841 tgggagctta aaaaataaaa aggtcgatat aagaattata tctgctacaa atgttgattt
      901 agtttcaaaa gtaaaagaaa aaaagtttag agaagattta tactatagat taaatgttat
      961 agaaataaaa ttgcctcctc ttagagaaag aagggataat ctagtggaaa ttataaaata
     1021 ttattttaat aggtactcaa gtgacttcaa cctaaataaa actatttcta aagaagctat
     1081 ggatgctatt gcaaattata gattcccagg caatataaga gaattacaaa atataattca
     1141 aaaaatcttg cttacttgta cagataatca tataacaata gatgacttac caaacattct
     1201 tacaaaaaat atacatatta ccaataatgg aaataaaaca catatatccc aaattaataa
     1261 ggttattata tctgactcaa aatctactaa ttataaaaat aaaaattttg atactttaat
     1321 taaagagtat gaaaaaaata taattttgga tgcttatgaa aaatttggta gttcttataa
     1381 agtagccaaa caccttgaaa tctcacagtc taaagccaat aggttaatta gaaagtatac
     1441 taatacttaa ttttaaattt gataaataaa aacttaacac tatttacata ttgtaaattt
     1501 ggccatttac tgttattatg aatatggttg aaattattta aataaatact gttgacttag
     1561 tttcatatat ttaccattaa acttttatca taaatatcat atcataataa tatatttaat
     1621 aagtctaata aactatacga ggtgtaaata tgttaatgaa tacccatttt attatggcaa
     1681 aatcaatact tgataatata gatgaaaata aaacattctt tataagcgaa aaaaatttta
     1741 tatatggcaa tataaagcct gatgcttttt ctaaatataa attaaaaaaa cattatttag
     1801 atgagtcatt taatatgatt gtttctaaaa taaactatct atgcagtctt aatatagata
     1861 gtctttctaa atacttttca gtaagtagac ttagtcagga actaggagtc atatgtcatt
     1921 ttttatgtga ttttttctgt gtagctcaca gtgaacgttg ggagtttaag catagcttaa
     1981 ataaacatgt tacttatgaa agagaacttg gagcatttgc aaaagatgtt gatttatcta
     2041 aaattaaatg tggtactata aatagtagtt ttgagaagtt ttttactaaa ctttatagcg
     2101 attacaaaaa gaaaactgat tatatgaatg atttaaaatt ttcagcttat acttgtaata
     2161 gtgtcataaa ttatatactt gactctattt tatctaatac aataaaatca tatcatatag
     2221 caaattgcgg ttaacttaag aatttttaaa cttatataaa cttataattg aatataatta
     2281 aaatgactgc tctatagaat ataattttat acttctctag gacagtcatt ttttatacta
     2341 actatttatc tccattataa catctatggc actttttaca ctatctacta cataatttgc
     2401 actttgcttt acatcttccc ttgccttaga cattgcaaaa ctatatttaa atccttctaa
     2461 catgcaaata tcatttggag aatccccaat agtagcaact tcatctggac ttacatttaa
     2521 cgtttttatt acatgctcta tacttcctct ttttgacaca cttttaggaa ctatatttaa
     2581 agtatatgtt ccagaaaaat atatttctaa gacttcacta aactcacttt ttagataaag
     2641 ttctattcta gataattctt ccaaattttc agaacatacg cctatatttc ctataagatt
     2701 atcaaaacta cttctttttt caaaatctgg atcaactata aagtcactag aataatgtct
     2761 aaaagcagtg gtatcactac ccacaattac atcaccctta tatttaaaat aaagttgtgc
     2821 atcacttgac tcaaatctat caatgacttt ttttactaaa ttatcatcta tcgttctttt
     2881 aaaaattaca ttataatctt tatcatatat aaatgaacca ttttcacata cataatatcc
     2941 attcatatct aaactatctt tgataatatt atgtgcttgt ttaaaaattc tacctgtagc
     3001 aacattaaac tgtactccct ttttttctat ttcttcaatt gctactatat cttcttttgt
     3061 tatggttcca ttttcatata gagttccatc taaatcacaa aatatatgtt ttatcataaa
     3121 ttaaaagcct ccaaaaacaa ttttatattt tactaatact ttaatcaaaa taacttaaag
     3181 cttttctaat tgtattctca actcattaag caaatctcta gtagctctta atcttatctt
     3241 tcttctatcc ccattaaata tatttttctt tactattgtt tttccattta tatatattgc
     3301 agtataaacc agtcccacag gcttctcttt ggttcctcca ccaggtccag ctactccagt
     3361 tgtagatata cctatattag ctttaagtcc tttagcaacg ccctcagcca tttctatagc
     3421 tgtttgctca cttactgctc caaactcttc caaagtttct ctttttactc ctaatctttt
     3481 cattttagaa tcattagaat atgttacaca accttcaaga aatacagagg atatacctgg
     3541 atagtttata agagatgaag aaaccaaacc tccagtacaa gattcagcca ctgctattgt
     3601 tagatttttt tctacaagta gcttagaaac agcatcttct aaagcagtct ccccttcaga
     3661 tatggaaata tcgccttcag tatatacata tttaccaact ctacttttta ttttttcttc
     3721 tactggtttt attaatttaa aagcttctct ttcattttca gcctttgcag ttattcttat
     3781 tgtaacttct aattcttttg catataaagc aactgttgga ttagtctgct cattaattat
     3841 atcgagaatt tctaattcta gatttgactc acctattcca tataatctta aagtcttaga
     3901 tactagcatt tcatttgtga attgttgtaa gtaaggtttg acactctcat taaacatagc
     3961 tttcatttct cttggaggtc caggcaatac tattataaat ttaccatctt tttttaaaat
     4021 tgcccctgga gctgtaccat tattattttt tagtactata gcatcttttg gaaagtacac
     4081 ttgtttcata ttattttcgg ccattttttt ccctgtttta acaaagaaag attccaaaac
     4141 ctctagtgaa ggtttatgta gttctaattt ctgaccaaag tattctgcag cagtttcttt
     4201 tgtcatatca tcaccagttg gaccaagacc acctgtagta attacaaaat cacttctctt
     4261 taagctttca tcaaaacatt ctaaaagtct ttgtttgtta tctccaacag tactctgatg
     4321 gtatacttct attcccaatg atgcaagttc tttagctaaa aattgagaat ttgtatttac
     4381 tatatctccc aataatattt ctgttcctac acttattatt tcagctttca aaatttatca
     4441 ctccttgatt tatagccatt aagactagtc ttaagttcat aacaataatt ttagcaaaaa
     4501 taataacata aataaagtta aaaaatcatt taaacatatc tttaaactag attattattc
     4561 taaaaccaca cataatataa acttcgtata tagtggtact ttcatcctca actgttaatt
     4621 catataatta agtatgtaaa taaaaagttt actttaaaat aataaaaatt aaaataggag
     4681 tgtataatat gaaaatagca gttataatgg gtggaatatc atcagagaga gaagtttctt
     4741 taaattcagg aaaagagata tataataacc ttgataaaaa taaatatgaa gttgtaaaag
     4801 taataataga tgataaaaaa gatatattta caaagatacc agaagatata gattttgcaa
     4861 tacttgcttt acatggaaaa tttggtgaag atggatgtat acaatcaata cttgagacta
     4921 tggatatacc atactctgga tgtggacctc tttgcagtgg gatgtgtatg gataaaaata
     4981 taactaaaaa aatgcttaga gattcaaacc tacctactgc tccatgggtt ttagtaaaat
     5041 cagttgatga aatagactat gatgaaatag ataatatagg ttatccagta tttataaaac
     5101 caaactcagg aggttcaagt gtagccacat tctttattca ttctaaggat gaagttgaag
     5161 aagcagttag aaaaggtctt gaagttgatg aatttgtaat gattgaaaaa tatataccag
     5221 gtggagaata tacttcattc atattaaatg gagaagtatt ccctacaata tctataaaat
     5281 ctgattcagg attctttgat tatgaagcta aatactcagt tgaaaaaggt gcaaaggaag
     5341 aagttgttta cttagatgaa gaacttcaaa aaagagtaaa cgaaatttct gagacttgtt
     5401 ggaaaatttt taattgcaaa gcatatgtaa gagttgatat gattataagt gaaggtattc
     5461 catatgttct tgaactaaat actcttcctg gaatgactca aactagttta attcctagaa
     5521 gtgctgctgc tagaggtatt aagtacagtg aactattaga taaacttata gaatactctt
     5581 taaattagat gaaataattt gaggtggtaa tttgatattt tcaaatctaa aattaaatga
     5641 taatgaacca atttatatac aacttaaaaa ttatataagt gatatgatat caaaaggact
     5701 aattccagac aatagtaagc tcccttctac aagggagctt agtcagcttt tacaagttag
     5761 ccgaaattct gtagttttaa cttatgaaga attaaaatca gaaggattaa tctactctat
     5821 ttcaggaaaa ggtacatttg ttaaatcaaa gaataagtct tcaaatacta catggtcatt
     5881 aaactgggat tgtttagaga atacatattc taaaaaagcc aatgaattgg acattataaa
     5941 aagtgaaatt ccttggagct ctgacctgat ttcctttaaa agtatttctc cagatggaga
     6001 tttattcgac atggaagaat taaaaaaatc ctttttaaat agaatttcac tagaaggaca
     6061 taaattactc aactatggat atgcacaagg ttataagccc cttattgact atttactaga
     6121 atatatgaca aacaagagtg ctgatataag taataaagat atactaataa caaatggatt
     6181 cacagaaggc tttgatatta ttatgtcatc ttttacccaa gaaaaagact acataatatg
     6241 tgaaaatcct actcataata ctgctataaa aattatgaaa tctcataacc tcaatatctt
     6301 aggtgtagat ataaatgaag atggtttgga cttcaatatg ctagaagaca atttagttaa
     6361 gtataaaaat aaaataaagt ttgcatacat tacaccctct tatcacaatc ctacaggaat
     6421 tgtaatgact cctgaaaata ggtacaaatt ctataatctt atgaaaaaat acaatatagc
     6481 aattatagaa gatggattta atgaagaatt actctacaat agttctcata tatttcctat
     6541 ctgttctcta gataatatga ataatggggt cgtatatatt ggtagttttt caaaaatttt
     6601 atttcctgga atgagaattg gatggatatt tgcagataaa aaagtaatca ataagctaga
     6661 aagtgtaaaa aggtgcagaa acatacatgt atccttttta gaccaaggaa ttttatttga
     6721 ttatttaagt aatggaggtt ttgaaaaata cattaaaaaa atacgtaaat tctatggaga
     6781 caaatttaac tttgcatata agtgtataaa aaaatatata aaaacagaat atatcttggg
     6841 tgatggtggt ttgcatatat ttataaaact aaaaaatata gatactagag ttttattaga
     6901 aaaatgttat gaaaaaaatg ttatatttat gcctggagat ttatttttta cagataactc
     6961 tggttttgat actttaagac tcggattttc taggttgtct tttgaagaca ttgaaattgg
     7021 aataaaaata attggagaaa ctataaatga aatgttctag gttaactaaa tattgttagc
     7081 ctagtttatt tttatgaggt ataatatata tagtttatat gaatctaata ctgggggata
     7141 atatgagtaa aaagttaaaa cgaagaaaaa ttaataaatt aaaattaacg attgtaataa
     7201 ctgtattatt tcttgcttta ttatctatat atgagatact gttctctaat cccaacaaaa
     7261 aaggtaatat aaagagtcaa gaaactatgc aaaataaaaa taatacaaca gatgattcta
     7321 aaaataatca aagctctacc aaaaagcagg aagataataa tatcaagcct actttttcat
     7381 attcttcaat accagataat ataaaaaata aaatgattgg taaatctatg cctactgatg
     7441 aacctataag ctttgatagt ctatcttatt taaaacttac atattatgga tttgatgaaa
     7501 aaactcatca aggagaaatg atagtaaaca gtgaactagt tccagaagta gtggatattt
     7561 ttaaggaatt atatgaaaaa aaatatccaa ttgaaaaaat taagttaata gatgactatg
     7621 atgctgtaga tgaaaaatct atgtctgata ataatacctc atcattttgt tatagaacta
     7681 tagctggtac aaatgtagtt tcaaatcatg gtaaaggacg tgctatagat ataaacccat
     7741 tacagaatcc tcaagtttct ggaaatgatg ttacacctaa agtaagtaca gtttatgctg
     7801 ataggtctag cacaaaattt ggaatgataa aaaaaggtga tgattgttat aatgcttttg
     7861 taagcagagg ttggagttgg ggtggctatt ggaaaaaccc agattatcaa cattttgaaa
     7921 aataatagat tagaaataaa ggtgaattat atgaatttaa tgacattgga aaatataagt
     7981 aaaagctatt ctgaaaaaaa attactagaa aatatatctc ttggaataaa tgaaggggaa
     8041 aaaataggac ttattggtgt aaatggtact ggtaaatcta ccttactcaa aattattgca
     8101 ggagttgaag aaagtgagac tggtaatata acaaaaacta atggtattag gatagaatat
     8161 ctacctcaaa atccaactta caatgaaaat tcaactgtac ttgaacaagt atttaatgga
     8221 acttctgaag aattaaagct acttggtgaa tatcaagaaa tcctagaaaa acttaattca
     8281 aactttacag aggaattaaa taaaaaatta ttatcagtac acgaaaaaat agatgcctta
     8341 aatctttggg atttagaaag tgaagctaag tcagtactta caaagcttgg tataaatgac
     8401 tttaatcaaa aaatagcaga actttctggt ggacaaagaa agagagtctc tctggcatca
     8461 gcccttatta caccttgtga gtttttgata ctagatgagc ctacaaacca tctagacaat
     8521 gatactatag attggcttga ggaatatctt aattccagaa aaggttcatt gcttatgata
     8581 actcatgata gatacttttt agaccgtgtt acaaatagaa ttattgaact ggacaaaggt
     8641 agattattca gttatgatgg gaattattca atatttttgg aaaagaaaat ggaaagactt
     8701 tcgctagaag caagtattga agataaaaga caaaatttaa taagaacaga gcttgcgtgg
     8761 gtaagacgag gagctcaggc tcgtagcact aaacaaaaag ctcgtttaca aagatttgat
     8821 gaacttataa atagagattc ttttaaacct gatgaaaatc ttgatatatc tgttgcatca
     8881 agtagacttg gaaataaaat aatagaaata cacaatatat ctaaatcatt tgaagaaaat
     8941 actgttatag ataatttaga atacacttta actcgtacag atagaattgg tataataggc
     9001 aaaaatggta tgggtaaatc tacccttata aatatactta atggagaaat agagcctgat
     9061 agtggatata tttcaattgg agaaacggtt aaaataggtt gtttttctca agacgactct
     9121 catatgaata ttgatatgag ggctatagac tatgtaaaag aagctagtga ctatatagaa
     9181 acttcagatg gtactaggat tacagcttct caaatgtgtg aaagattcct tttcaatggt
     9241 actatgcaat atactttgat aggtaaatta tctggtggtg aaagaagaag actacatctt
     9301 cttaaaatcc ttatgacagc tcctaatgtg cttcttttag atgaacctac aaatgattta
     9361 gatattgaaa cgcttaaaat attagaagaa tacctagacg aatttaaagg tgtagtagtt
     9421 acagtatcac atgatagata tttcttagat agaatttgta ataaaatatt tgcatatgaa
     9481 ggtaatggaa aaattcatat ctatacaggt aattacagtg attatcttat ttatagagag
     9541 attcaaggaa ttgaatttga agaatctaaa aaggaatcag ttaaaaaaga tgactctact
     9601 accctaaaaa aagaaaaacc taaaaatgat aaaaagctta agttctcata taatgaacaa
     9661 agggagtttg aaaatattga ctctgatata gaaaaactcg aatctaaaat tgcaacactt
     9721 gatgaaagta ctgcaaaatt ttcaactgat tttactaaac ttcaagaaat tttagatgag
     9781 aaatctaaac tagaaaaaga acttgaatac aaatatgaaa gatgggaata tttaaataat
     9841 ctagctgatg aaatagctaa taataagtga gattaaaata atcttaagtt taataagttt
     9901 tgctttaaat ctgtacttta taaacatttt aaaggaagtg tctcaaaatg aactttttag
     9961 ttttatgaga cactcttttt tatgagaaat caaatatgtt ttcatcattt caacaataca
    10021 aaagaggctt atctctattt tgagacagcc tctttcataa ttaccttcta atttatatgc
    10081 tctaatatat ctataataat tcttattata tctatatatc cagtctttca atatagtgac
    10141 aattaacttc aatatgattt aaaaatacta acaacctttc ataaagtgga tgagattctt
    10201 ctccatattt agcttctagg ttcttgccta tttctctaac agtcctttga ccatcaattt
    10261 gtaaaaaaac aaaactggaa tattcatcta gttctatttt tttgtattta ggtattctag
    10321 cttttaactt tctaaaaaac ttttgaattt tatggtcctg tttttcaagt atagtaacaa
    10381 cactattttc agctacttca tattcaagtc catcagtaat tttaaataca atatctaaaa
    10441 catcattact cttgctcatt attttttact ttagataaaa caataggtaa tgctgtaagc
    10501 acaactaaaa ctaccaataa agcaattgcc attgaatttg ttgcagcaaa tccacttggt
    10561 acctttggag ttacaactcc agttacttgt aatattatac ctattaaacc aatgatagaa
    10621 ccaccagcaa caagaccaga agataaactt ataccattag aaactttaac ttctttttct
    10681 ttttcagttt tagatactaa ttcaacaaat aaacgaatta atgctccaac taatattata
    10741 gaagttgttg caattggtaa atagaatcct atagctatag tcattattgg taaatttaat
    10801 gcatatagaa ctattgccat aaatactcca acaataatca taacccaagg taaactacct
    10861 gacataatac cagaagttaa tgttgacatt aagtttgctt gtggtaatgc aaattgtaca
    10921 ttatcaccag ttgctctaag ttggtcagaa agtaataaaa ttactcctac aacaactata
    10981 acaccaacta tacttgcaat tgtaaagtaa cgttgcattt cgtttttgct accaccaata
    11041 atataagtta ctttttgaga ttgagtataa cctccagcta tagcaatagc aacaactata
    11101 aatgaaccaa ataaaagtaa tgatttatta gcttctaaat cagtccatcc cattataaca
    11161 aacactaatg ttacaataac tagagaagct atagtcatac cagaaactgg aagatttgat
    11221 gttccgattg ttcctgttaa acgaccagct actatgacaa ataataaaga taataaaaga
    11281 gatatgatag ctccaataat tgccataaca atgttaccag aaattaagaa tgctgctaaa
    11341 aatccaataa ctactccacc taataaaagt atcatttgta tagatgaacc ttcttctccc
    11401 tctccagcac ttgatttagc tttaagtgtt tcttttatag aagcgattat agttggtata
    11461 agttttattg caccaataat acctccacaa agcatcatac cagcacctat atattttaca
    11521 taactactag atattgctcc aacatccatt tggttaagag gcattgcagg attattccat
    11581 accatagctc catctttagc catatctgta aagtatccta ttaaaggtgc tataccaaag
    11641 tttgataaaa tacttcctgc aaacattgtt aaagaaactt caagccctac tataaatcca
    11701 attcctaaaa gtagaggatt tacttcaata tcaaatttcc atttgtagaa tttgcttcct
    11761 acaagactca ttacattgtt tgctacattt aagaatgaac cagttaatac agtaataaat
    11821 ccacttatta caaatccaat tcccatgtac ttaattgaat ctccaccagc ctctgaagca
    11881 acaagtgttt cagatatagc cattgattct gggtacataa gtttaccatg ttcttcaact
    11941 attagatagt tgtgaactat agcagcaatt cctataccaa ataaaactcc accaactcca
    12001 acagctaagc cttcaaagaa tgtaatttgg cttccaatta atataactgc tggtaatacg
    12061 aaaataaatc cacttgcaac agattctcca ccacttgaca taccttggat taagttttta
    12121 ccaagaatac cctttgaacg agcaaatgct ccaacaaaag cagaacctat tatagctcct
    12181 ggaatacctg cggcaactgt aagaccggct ttcattccag agtaagttgt agatgcggca
    12241 aaaatggcag ctaaaataat accgataatc agtacagcta catttccacc ggttctggac
    12301 ttatctgtga tatatggaac gtaatctttt ccactaacct caccataagc acccttaggt
    12361 aattttttgt tcataaaatt ttcacccctt ttcaatttgt atatattata acatatatac
    12421 atataaaact taacttttta ttattttaca aatagcgagt caaaactact aaaatttaac
    12481 cctatattat taatttctac ttttagatat ttttatgcta attttctttt tatttttcaa
    12541 ttctactaaa aactattaag atataaaata aaaaaatttt ttaatgtaaa attaattttg
    12601 taaaaaaaat gctttaaaac acggaaaagt ataaaaaata caataatcat tcttataaat
    12661 aaattttcac catagtaaat atatacagaa aattcttgtt tttatgcaaa ttgattcttt
    12721 tataaaaata agtgtcataa agaaatgtct gaaatcactt tatgacacct atcataataa
    12781 aaaattattc tggtaatgct cttttattta cttttccgat tggtgtaact ggtatcttat
    12841 ctatcctcat tattattgtt ggaatcatat atggaggtag atgctcattt aaatattctc
    12901 taatagttct ttcatttatc ttaccactat cttcctcatc aattacataa tatgcacata
    12961 tatacatatc tccatcatca tttgttctcg atatacaaac aacactttta atttctgtaa
    13021 tattatttat tatattttca atctcatata gctctatcct aaactcatca atttttactt
    13081 gattgtccat ccttccaata taatctaaat ttccatcatc taaccatctt actaaatcac
    13141 cagttctata cattaactta ttatgttcac aagttttgta aggattctct acaaatactt
    13201 gctctgttag ccctttttta tttaaatatc ctcttgatat ttgaatgcct gatagacata
    13261 gctctcctgg tgctccaatt ggacatagtt tgttcttttt atctaataca tatgcataag
    13321 tattaaatat aggttttcct attggtattt ttccatattc tctgtccaca tgaaaagaag
    13381 tcgatacaac agtaaattcg gttggtccat actcattata tagttcataa ttacgctttc
    13441 tatatccttt taatctttct cctccagtct gtacaactct aagtgaatta ttattgcaat
    13501 tttgtataaa taaccttgca aatgctgttg gcaatgctag tatagttatg tcattttcct
    13561 cacagtattc atttactgta tattcatcaa atttagcttc ttcaggcata atatctattg
    13621 atgcacctac aagcaaataa gggaatagtt gtttcattac agcatcaaaa ctgaaactta
    13681 agtatatacc acatatatca ttttctgtag cgcttataga ttttacgcta taataacaca
    13741 tatttactaa actcttatgt tctatagcta tggtctttgg cctaccagtt gaccctgaag
    13801 tagatatcgt atatgcacaa tttgatgatg ttattttttc agttaattca tatccaagct
    13861 taaacatttc ttcatcattt atatcgagta ctatggtatt ctcatttaca accttacttt
    13921 tgagttcatc tgtagttatc actacttttg cattactatc ttcaagaagg taattcaatc
    13981 tttcttctgg attacttgtg tctattggaa aaaatgctcc ccctgatttc ataatcccta
    14041 ttgctgtaat aattatgtta atatctcttg gtaacattac agctactatg tcttcacttt
    14101 ccactcctat atgatttaaa taacctgcaa gtttatcagt tacttcatcc aattctttat
    14161 atgttaactt tctattttca tccctagcag ctatattact aggtgttacc ttaacttgct
    14221 ccctaaataa atcaacataa cttttatcat agggtacttc atattgatta ttattaaagt
    14281 cttccaataa tactttttct tcttccttac atacaaattc taaatcttct attaaaatat
    14341 cattatcttc tattatttgc tctattatat aatcaaattg atttaaaatt cttctaatag
    14401 tttctctttt atatatagat ggtttatact ttatttttat ttta
//
LOCUS       DAEMHZ010000108        14300 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000108 DAEMHZ010000000
VERSION     DAEMHZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.108"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            236..1210
                     /locus_tag="KPK72_002843"
     CDS             236..1210
                     /locus_tag="KPK72_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490041.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1326..2387)
                     /locus_tag="KPK72_002844"
     CDS             complement(1326..2387)
                     /locus_tag="KPK72_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2490042.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKTLCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(2999..3850)
                     /locus_tag="KPK72_002845"
     CDS             complement(2999..3850)
                     /locus_tag="KPK72_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2490043.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3867..4787)
                     /locus_tag="KPK72_002846"
     CDS             complement(3867..4787)
                     /locus_tag="KPK72_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG2490044.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="KPK72_002847"
     CDS             complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="KPK72_002847"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG2490045.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5910..7007)
                     /locus_tag="KPK72_002848"
     CDS             complement(5910..7007)
                     /locus_tag="KPK72_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2490046.1"
                     /translation="MGRLGISIYPEKTASKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7240..8586)
                     /locus_tag="KPK72_002849"
     CDS             complement(7240..8586)
                     /locus_tag="KPK72_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2490047.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8675..8983)
                     /locus_tag="KPK72_002850"
     CDS             complement(8675..8983)
                     /locus_tag="KPK72_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2490048.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9236..9634)
                     /locus_tag="KPK72_002851"
     CDS             complement(9236..9634)
                     /locus_tag="KPK72_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490049.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9636..10796)
                     /locus_tag="KPK72_002852"
     CDS             complement(9636..10796)
                     /locus_tag="KPK72_002852"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG2490050.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISENGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11227..12246)
                     /locus_tag="KPK72_002853"
     CDS             complement(11227..12246)
                     /locus_tag="KPK72_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2490051.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12240..12920)
                     /locus_tag="KPK72_002854"
     CDS             complement(12240..12920)
                     /locus_tag="KPK72_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490052.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(13072..>14300)
                     /locus_tag="KPK72_002855"
     CDS             complement(13072..>14300)
                     /locus_tag="KPK72_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490053.1"
                     /translation="ITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNII
                     AINNINYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMD
                     KDEVNKKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVN
                     GNDKELKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLG
                     TGLNQSIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQN
                     GYKAQGESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTE
                     KDINRAILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFII
                     YGAVSVNKFKSVVYKN"
BASE COUNT         4599 a         2499 c         1509 g         5693 t
ORIGIN      
        1 ttcatttgct atttaaaatc aattttaagt tttcttctta taataaactt cctctttgtg
       61 gtctcatgtt agtctctaaa tttaaaattt tacaaaaaat tttcataaaa taaaattata
      121 ttgattgtat ctaaacaaat agggcaataa aataattaag tatagttact tacctttcaa
      181 gcaatattag ttttatgtta gaattttatt atattgattt acaggaggtt tgcacttgaa
      241 taatattaat tcacttaaag taataaaatt atctcttgga tgttctcttg ctatatttat
      301 tgcttggcta ttaaaattag aatactcaat ggttgctgga gttatagtac tacttacagt
      361 aaaggacact aaaagagaaa ctcttaaagg ctccattgga aagatttatg gttttctttt
      421 atgtactatt ttttcatatt tatgttttaa tattttagga tataatttga cttcattttc
      481 aatatttata tttataatta taccactttg ttttttgtta aatattcaag atgttattgc
      541 tatgtgtgta gtcatagcat cccattacta ccttcaagga gaaacttcta tcaaatggat
      601 tttaaatgaa gctggaatat ttgcagtagg aactggaata ggtgtcttaa taaatatgta
      661 tattccaaca aatatacata aaatccatga agttcaaaaa aagttacagg aagaagtgag
      721 tattgtactt atagacattg cagatattat tgtaaatcct aaaaaagaaa atggatatag
      781 tagagattta tatacattaa attcacttat tgacagcagt atttctgaaa cttacgataa
      841 tattaataat actttattaa gtgatactag attcttctta gagcatatgg atattataaa
      901 aagtcaacgg gatatcttag aaaatttgta tagttatgta tctcaactaa attctacacc
      961 accacaagct catattctat ctgcttttat acataagata ggttatactg aatttgaagc
     1021 tgaaacaggc aatcttcttc ttgaagaatt aaagagacta atgcttagta tgaaaaacca
     1081 gcctctacct gttgacagaa cagagtttga aaatagagct attttattcc tatgcttgac
     1141 tgagttaaaa cagtttttag ttaatcgtaa acatgctcag atgcttagag ataataattt
     1201 ttataaataa aaaggggaga aatctagcag ttgacctgac tacaaaatat cagatataat
     1261 attaaatgcg cccctagtaa gagggcgcaa tgtttataac ttattctact gtcatgatta
     1321 aatcattatt catatcgaac atacattatt tgaccaagtc caatattagc tttttcatca
     1381 gtgatttcta ttttaatagt cacatcattt tttttacagt acttcgctaa ttctgcacca
     1441 gaacgatttt taccactaaa atctaaagaa aatggcttat catatttaaa aggatattca
     1501 acaggtttac cctctggtgc taatgcaaac atcccatatg gtgtgttcat aaatcctatt
     1561 acagcaatcg taagatttga tgggtctaaa tttgattcac tacctgtaaa tgtaattgta
     1621 tttactgatg aattaactac actacatcga acaccttcat acattacatg tttttcattt
     1681 atatcttctc ctttttttcc aaaatttcca acagcctttg ccccttcttg atgtggtaac
     1741 atagtgtgct tcccaggtgg tgctggtttc tgtgtctgtt ctggttttac tgtctgtact
     1801 attgcaccaa cttttggata tgctgtctta tcaaatggta atgggtggct aagactcttg
     1861 tatatgatat cctttcctgc tgatttatta gcatcaatat ctacatgata caaacgccca
     1921 tcaccaaaac ggataagatt ccactggtgg tcatttgatg tgctgtgtac ataataagat
     1981 tcaattccca tagcatcaca caaagttttc atgctcctag ccattcctga gcatgcacct
     2041 tttttagtaa ccaatgtata ggcagtattt gctccctcta ctgcattcca gtcaacatat
     2101 gttgtgttat ttttaatgtg attgtaggca actaaaatct tagcatctgt ggtcatattc
     2161 tttgtgattt tcttgttcat aaaatctgct gttgcttgtg ctactgagtt tagttgggct
     2221 tgtgacagct ttggaccata tacgctattg gttccagtga catagctttt tggtgttttt
     2281 gctgttacct ctttggctgt cataccataa actgtcatac tcatacctga gattaaaact
     2341 attgttgtaa tcaataactt ggtagttaac ttcctaattt tcttcatact taatattctc
     2401 ctttataaaa cattgttact tcctattctc taccattaat aaaattaatt taggatttaa
     2461 aaaggtggat tgtcaacatg tatatttctc cattcataca ttcgtgaaat atttaaattt
     2521 tatggaaaat ctaatagata ttttctagat aatttgtata caatacaact cgcaaatcag
     2581 ggtatttgct gttagtttga gaaaatgatg atgattaata cattcggaat ctttgaccta
     2641 taataaatac cattgtcttc aatcattaat aatggtaaga gtaaagagaa ttatcaataa
     2701 tacagttaaa atcaactttg tatacatttc atacattctc tcccaacaac attatacttc
     2761 ttataatttg tatattcaat acgttttctg ggaaaatata ggatatatct ataaaaatat
     2821 aaaaaaggag ttgaacttaa aatttgatta aaaaaaatca attttaagac aactcatttg
     2881 tattataaaa atgctatata ttattattaa gtaagtcaat attttagata tttatctatt
     2941 tcttattgac ttacgcttat agcctaactt atttgtttag ttgatatata acctagtttt
     3001 aagcattatt aatatttaaa taatcaagta tcctttttgt ttcttgtagt ttctgttctg
     3061 cctcatttaa gttttcttga attatagaaa tatatattat atctatcaac gtaagcattg
     3121 ctattcttga agaaatagct ccttctctaa gtcttttttc tatatttggt actactagct
     3181 ttaaatcact aatcttacta agatgattgt cactatattt agttatacaa atgaccttac
     3241 atccttttaa ttttgcattt tctatagact ttattacttc ttttgattct ccagagtaag
     3301 atattgctat agctatatct tctttatcta catttgaaga taccataagt tgtgtatgac
     3361 tatctaaaga ggtaaatgcc tgtttattta tccttaaaag tttcatctgt aaatctagtg
     3421 caactaaagc cgaaccacct actccaaata tatatacatt tttagccttt attatctgtt
     3481 caactgcttc ttttattata tctttttcat tcaacaacct tgtttggttg atagtctcta
     3541 tgtttttgtt tgtaattttg tttataacac tatctatatt acaatcatag ttttcatcta
     3601 catatgtgta ttcattttct ttgcccatca aatctttagc tatctcaatt tttaaatccc
     3661 caaaacccga atatcccatt tttctagaaa acctaactat acttgctgta cttgtttttg
     3721 agtttgacgc caattctgat acagaaagct tacttatagc ctctgtatta ttaagtatgt
     3781 aatcagctat ttttttatct acttccgtta aatcttcata tatctgtttt aattttaaaa
     3841 tgcaactcat actctatcac ctctacctat ttactataat atttcttctt agcaagtaaa
     3901 catgctccta aatctggagt caacactggt ctcactatac caaatctcgt tttattcaaa
     3961 tattttttaa gtggctctaa tattaaatct cctgacttaa acactccacc tgaataagat
     4021 acctttatct ttgtaccttg ttcaaaatga tgctctaatc ctattataag cctacttaat
     4081 tcataagctg catcatcaaa tattttacta gcagtattat caccttcaat aactgctttt
     4141 aaacatatcg tagcaaactt tgctatttct attctatctc cttgtatttc atcatttaca
     4201 tattttatta tgtcataatc attctctata ttaagatgtc tttttattaa atcatataat
     4261 tttgtttttg aacaccttcc atcagactgc attgtgaaca tttttaaggt agccattcct
     4321 atataatacg ctgaaccttc atctcctaat acatagcccc atcctccaca tctgtgaaga
     4381 ttcccatctt tatctaaagc ctgtgctatt gaaccagtac ctgcaattat atttatacct
     4441 ttttccccat ttaaagaacc tgctaatgct atttctacat cattacctag agtataattt
     4501 atatgactat atgcattttt tgttgctact tctaatgcat acaatacttc ttttattttt
     4561 ccatatccag caagtccaac aaatgcatac gttatttctt ctacatttat cttagcatct
     4621 ttacaaactt cttccaaccc agttattaat aatttcgtta gattatcaaa tcctatttga
     4681 ttataatgac aagttccttt ggttatggtt cctactatat ttaattcttc atctactaat
     4741 gtaaatgttg ttttcgttcc tccaccatct acacctaaaa aatacatact atcctcctac
     4801 aatatcttta ataattcttt caatacttaa tatctaaaaa taaccttcca atgctgtttt
     4861 tttaatattg aaaggttatc agtactatta tttattttac aatttttcta ttgattttaa
     4921 agcttcactt atataacctt cttgtctatc taggatactc tttgcactat ccttatctaa
     4981 accacttttt atcataaata tagccaattt aacgctatta tctgcttctt ctaagaattt
     5041 ttctgcaaca tcatatttta catttgtagc tatcattatt attctttttg ctctttcaac
     5101 caacttttca tttgatgtct taacatctac catcaagttt gagtatactt ttcccatctt
     5161 aaccatagaa gctgttgtta acatattaca taccattttt tgagctgtac cacttttcat
     5221 tctagttgaa cccattatta cttctgcccc tactataatg tttattggta aatccgcttt
     5281 tttactcatt tcactattcg gattcatagt tattgatagt actgcacacc ccaattcctt
     5341 agcatactcc attgcaccta taacataagg agtccttcct gatgcagcta taccacatat
     5401 aacatcctta gatgtaactc ctctatctat tatatcttgc tttccaagtt ctttatcatc
     5461 ttcagcacct tctattgctt taaatattgc ttgatttcca ccagctatta tagcttgaac
     5521 aagctcaaaa tccactccgt aagttggagg acattctgtg gcatctagta ttcctatcct
     5581 cccactggta cctgctccta tatagaataa tctacctccc tgtttcattc tatctactat
     5641 tatattaaca gcttttgcta tactctcctt ttgttcttca acagcatagg ctacagtttt
     5701 atcttcgtta tttattaatt ctattatgcc taatgtatct tctttatcta tttgcaatgt
     5761 attcttattt ctaccttctg taactaaatt ttctaaagtt tttattaaca tatcatttcc
     5821 tcattttctt aatattaaat ttttaaacac attttaattc caactttcat aattatattt
     5881 ttataataaa ttaatatttt cataataaat tattttgttg agggtttaaa attaaacttt
     5941 tgccatggtt taatataatc taatataaat aattcttctt ttcttatata tcctactaca
     6001 ttcgatttac cactgttttt catatcagtc aaggctattt gtaactcccc tgcataatga
     6061 ccatattcac tactttcaat tactatatct cctcttttta ttattttagg agtattaaat
     6121 agttcaaatt tgtatccttt atatttaaca cgagattgag tagaccttat cagactatca
     6181 gatatatccc ctctattaaa atgtatttct tcaaagagta tcttcttctc aacttctcgt
     6241 atattttcct ctattgctac atcaaatgta accatatctt ttctcataga acctaatacc
     6301 tttagctcct catatgttgg ataacaattt gatattatta tatcatttat attattcata
     6361 gctattatat gctttacttg aatatctata ggtagatttc tgtgcatctc aattgttgga
     6421 agcccatcag ttacaggcca tggtccaaat gtagtttcat tttgacttgt tacaaaaact
     6481 gctgttctta atccatactt attaaatctt tctgtacatt ttataaagtg ttctaaattt
     6541 aatccactat atctatgtgg ataaaagtta tgacatccta tcaaattttc tttatttggt
     6601 tggtagtcca ttattgtatc tatataatta gtattattac tcatattgat ttctatttta
     6661 agattttgag gattaaaagt cattaatgcc tcttcagaac ctgtaaatcc catatcaagc
     6721 cttattccgt ctgccttcgt ctttttaaaa aaagtcaaat ctttgtagct tatttctaat
     6781 tcaccaaata ctctgggatt tacatcaagt attacctcaa aacctttaaa cttagcgtat
     6841 tcatttatac ttttaaattt tttaattata ttttctttag tatctttaac tgataataaa
     6901 catgaaaata tacgtgtaaa tccattttca gcagctttat ctatataatc atatatttcc
     6961 ttagatgctg ttttttctgg ataaattgat attcctaatc ttcccatctg tgtcatctcc
     7021 ttatatccta tatgtgttta aatttatttc gaattacacg aaactttttt aaaaatttgg
     7081 tgaaaaaaag tttcatatat atttataaaa aaagaaaagt ttaccttttc tttttttata
     7141 aacaatcaac aaaatatctt cgttacttat ttcataactg ttatactttg aaatttatac
     7201 taatctaata tagtttttaa tttccaatat agtttctaat taataagctt cttgctcttc
     7261 aactttttct tcatgaagca acctcttatc gtacatcttg acaaatggat agtaaattat
     7321 tattgatata gctatacata ataagttaag aactgaagct ctccaatctc caccagtggc
     7381 taaatatgcc cctactggtc ctggtaatgt ccaaggagca gtaactacta ctctggatac
     7441 taaattagca ctcatagcaa accatgaaac tgttgccatt actattggtg ctacaacaaa
     7501 tggtattatt aatattgggt ttaatactat tggcgctcca aatattattg gttcatttat
     7561 attgaatatt gctggtatta cagttgcttt tcctaaagac tttccataag ctgattttgc
     7621 tctaaatgca agaagtaaag ctaatcctat tgtagctcca gaacctccaa tccatataaa
     7681 ccattgaaaa aagggttcag cagcaatacc tggaactgct tgacccgcag catatgaagc
     7741 tgtattagca tctaataatt gtatccataa aggtcttgcc aatgaaccta ttattgatac
     7801 accatgtatt ccaaatgacc aaaaaaacgt tattaaaaat attagtataa ttactgatgg
     7861 taatgtgtca gtggctgaaa ctaatggagt tactaattta gctatgaagc catgccaatc
     7921 aaaccctagg aaataagtaa caatagccat taaaattatt attaatgcgg ctggtgtcag
     7981 agcttcaaaa gaccttgtga ctgatgctgg aactgcttct ggcatggtta tcttgaattt
     8041 agatttgtca gtaaatctat aaatctcaac acctattata gatgttacaa tagcaacaaa
     8101 cattgaacct ccaccaagat tagccattgg catttgccat ccttgaggaa ttgaattata
     8161 ccaagctaaa agttctggac tcttttcaac tatctttaat aaatcagggg taagaccctc
     8221 tattgcagta ggaactattg tcaaaaggaa tgtagctgta gctaataaac ctcctgtcaa
     8281 tccatctaac ttatatgact ttgccaaact ataacctatg ccaaatgtag aatatactga
     8341 tataataaaa aatgtcattc tatatggtag taatattgta actgcatttt cctttagata
     8401 ctgagttacc ccccatgaat ctggtaatgg aggaaatgct aatatcaaga aaaatgaccc
     8461 aactattata agtggtaatg aagctacgat tccatctctt acagctctta agtgacgttg
     8521 ttctgccaat ctggccatag aagttgataa tttagtgtct aaaaattctg taaatttatc
     8581 tagcatcttt tctctcctta attaatattt atttttatta tcattaaaat ttttataaaa
     8641 ttaactttct tctaatctaa caacactgca actactattt actcaacagt tcttgaactt
     8701 gttttaataa ctttggtcct cctaatggac tataagcttg tgcttgtatt aatgcacatg
     8761 gtacactaac ctcatcagca aaagctttta aatagtcaaa tctatgtctg acttgtggag
     8821 ctaccatagc aatatcccat ccatttttaa cttcattctc aaactcttga gtaccaacag
     8881 ccaaaacatc tatatctatt ccattttttg ctccttcttt ttgtaatgca ttaactgcta
     8941 tagcacttga catccctgat gaacaaacaa ataatactct catattgatt ccccctgttt
     9001 ttttatttct tttcaatcat tatagcatgt atttttgatt acaggaatac gtttgtatat
     9061 attttttcat attttacatt ttaaattgta attttatttc atgcatttat atcttgcttt
     9121 atttatctaa tcattctttt gtctaaaatt acacactact taaaatactc atatcttttc
     9181 ttaaaaaaga aaaaagacta tcttattatt aacataaaat agtctcaata cttttttaaa
     9241 tatgtattcc tttatatatc cagaagtaac ctattattgc aaatgataaa gcaaataaaa
     9301 tatacagtct aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag tatcttgtat
     9361 atgcaactgc aaaagatgta attaatgaat ttcctataac aactccaaat ctattatcta
     9421 ttccaaatat agataataac aaaggaaaaa ctacagtact tacaagagat gaaactatta
     9481 gtattaatcc acttttaaaa cttatagttg gaatattata atctgttctt tgatttttct
     9541 ccatttgttt cttagtattt ttattacttt ttgtactttt tgcattttta taattattat
     9601 ttttctttct tttctttttt ttattattag acatgtcatt tacctccaaa aggtttaggt
     9661 gttatattcc ctaaaataac tttattttta gcccctgttg cagatattac attagaaaaa
     9721 gaattattta atgtttcatt gcccaagatt acaaatgcca aagcctcctt tgcatcagaa
     9781 gaatacccat attcatcttg tgttaatact tcaacctcta ataataactc ttttatatat
     9841 cctattaaag ttagattatg agctcctcca cctccaatta ataaagtatc tacattaacc
     9901 tttggaagta tgaagttttt ataattataa tatatagctt ttgccgtaaa catagttact
     9961 gttgctatca aatcatattt atctacatgt ttatatttgt taagcatgtt atccaaataa
    10021 acttgtccaa atacctctct tccagtgctt ttaggtggcg cttgactaag atacttatga
    10081 ctcatcaaat cttttaacat ttcttcatta attttacctt ttgatgcaaa gtatccattt
    10141 ttatcatact ttctattaaa taatctttga catactccat ctattatcat atttcctggt
    10201 cctgtatcaa atgcaaatac atcatctatg ttacatgatt taggtattat agtaatattt
    10261 cctattccac caatattttg aagtgctaaa tttttattgt tatcactata taataaaaat
    10321 tcactatatg gtactaatgg agcaccttct ccacctgctg ctatatccat tactctaaaa
    10381 ttagatatta cctttgtatt tgtttcatat gctataactg ctggttcacc tatttgaagc
    10441 gttgaagata caaaattatt ataacttctt ggtatatgaa agattgtttg accatgagat
    10501 gcgataaaat ccaaatttgc tatatgaaag tttgctttat gacacactga ttttacagct
    10561 ttactaaata aatatccaag cttaaaattt aagctacaga ttaaatcaac actcgattcc
    10621 tcttctatac agcactttct tatttcgtct tttatatctt taggaatttc tagagttata
    10681 aattctattt gcttaacttt agtatttcta ccattctcac taatttcagc tagtactgca
    10741 tcaactccat ctaaggaagt tcctgacatt aaacctacac tatacatagt tatcatcctt
    10801 ttaagtttat agttatccct ccccaaatca aggtattaag tttaataatt ttagaattaa
    10861 tcttatttca tctttaatta cgataattta ctcttatgaa attaattgag ttaatcttat
    10921 acacgttttt taccttagct aaatcttaca ttaatttatt ttcagtgtca atgtaatacc
    10981 atgtaatttt ttttcacaaa caaaacatat tctctctcca aaaaccatat taaaatagtt
    11041 actacaaata aaaaacatac caggtaccta tctgatatgt ttttcatata taataattat
    11101 tattttataa tatagataga acatcattta aggcactaat taaatcttca ctattttcaa
    11161 gaactaaatt tattcttatt gtatatgtcg atttgtatat gatgatagat tttatagtct
    11221 aataacctaa ctatctaggc ttaaataatc cgatatatca taaaagtaga tattaaaaat
    11281 tgtatatgaa ccaattttcg aacatacctc aatctcatgc cctaaactat ctatcatttt
    11341 ctttacaaaa tacatcccca ttccagtaga cttatacaat tgatttcttc catttttccc
    11401 cgtaaatccc ttgtcaaaaa ccctagatat atcttcctca tttataccta taccaaaatc
    11461 ttttatagat aataccactc cattttctaa cttttttgaa ttaaattcaa tcttaccaac
    11521 ttcctttgaa tactttatag cattattaat aatttggtct aaaacatatg acatgcattt
    11581 agaatctgtg taaacatcat tttctaattc attcaaacta acttctacat tattttttat
    11641 aagcaaaaat gaattatttt ttatagacat ttttaccaat ttttctaaat taaatttatt
    11701 tataaataca tcttcaaaca tagtgttgct tctactacta tacaaaatag agtttactaa
    11761 aaaatttatc tttgcaacct gattcttaat atccaaacta tcttctatgc tacttaacct
    11821 atctgtaatt atacttaaag aagatattgg aatttttatt tcatgaaccc atttagctat
    11881 gtactcatcc aattctttta atgattgttt ataagtctct atgtcactat tattttttat
    11941 ttttaagttt tctataatat cttttattag attttcttca aaagattgtc catcaactaa
    12001 ataactgtct atttcaccac tattttctat acaattatat aaatttgtgt aactatttct
    12061 aaaatttata taatctataa caaaaaatac cattattata aacagcacta aaaaatctaa
    12121 atatattaac tctgaatatt tatttttaaa aatatctata gcaaataagt agatatttat
    12181 tatactaact acaactaaag gtagagcaat atacttagac ctacctttca aatattttac
    12241 tagcattgta ttatatatcc tatccccttc ttagttttta taaaatcatc aaaacctagt
    12301 tctttaactt tacttctcaa tctattcata tttacagtaa gtgcattgtc tgtcacaaac
    12361 tcatcattat cccatagagt catcataaga ctttcccttg atacaaccct attcttattt
    12421 tttaataata atgttaaaat ttttatttcg tttttagtca actctatact tttatcctta
    12481 tacttatgtt ttataatccc tttccccaca tcaaacatca aatcttcaca atacaaaata
    12541 ctattagaat cactataatt atagactctt cttattatac catttatttt ggtaaccaat
    12601 atttctatag aaaatggttt ggtaatgtaa tcatctgctc cattatttat gcccattatt
    12661 aaatccatat cggaatctct tgatgataaa aatattaatg gtacatttga aatagctctt
    12721 attttttcgc accagtaaaa tccattatat aatggtaaat ttacatctaa aagtactaca
    12781 tctggttttt tatctataaa ttcatctaat actacctcaa aatcatcaat taattctact
    12841 ttaaatcccc atttttcaag tgctaatgca atctcttttg ataaatcaat atcgtcctct
    12901 actaataaaa ttctatacat ttgtatattc tccattatcc taatatttca ctatgctatg
    12961 aattattaat atcataacat attttatatg aatttattac ccaaattatt tagttaatca
    13021 acaaggagac tcttattaca agaatctcct ttctctttat taatatatca actaattttt
    13081 atacactaca ctcttaaact tattaacaga tactgctcca tatattatga atattatcaa
    13141 acatatcact aaacttagta tatatatacc aaataaatcg gtcatcaata aatctcctgc
    13201 taatctcaat gcaaaataac tagatatagt tgcacttaac attggcaaag tataaaaaat
    13261 agctacttct ttaagtatag ctctatttat atctttttct gtagtaccta tttttaacaa
    13321 aatcttatat ttctctttat cttcatatgc atcactatat attttcatat atattatact
    13381 tccagctaca attgcaaaga ctaagaataa aaacacaagc actacatatg caaacttcat
    13441 ccattgactc tcaccttgag ctttatatcc attttgaata tacacatttt ttaaacttaa
    13501 attcttttca atattgttga acattacttg actattttct tcttgaaata cctttgcacc
    13561 ataaaaatac acttgttgcc ccttatctct tagtttaaaa tatgtatcat catttaccac
    13621 tataatactt tgattaagtc ctgtccctag tactggaact tttacttctc cccttgaaat
    13681 attatataac tgaccatcaa gttttatctt ggcttctttt gtagctattg atgctatcgt
    13741 ttgagggctt tttaactcta ttacattatt tccactaact aatctttcac catactcttt
    13801 taattcttta tcattgccat tcactttaag agtattacaa aaatcactat atttcattat
    13861 agtatctgtt gtatcataag cttcttttat atctgttttc tttaaaattg ttagttctgg
    13921 atttactgag tactttatct tattagtatc ttttattata ttctctattt gttttttatt
    13981 cacttcatct ttatccattg aaactatgct aatagtgtac acatttcttt gttcttgggc
    14041 attcttatgt atacgcttca tcgcaactga agcaccaaga gttgatattg ttgtagttat
    14101 aattatagct attatagcat aagttttata gtttttattt aatcggtagt ttatattgtt
    14161 tattgcaatt atattatttc cattaaataa tattttttta tttttgatta taatatcaaa
    14221 tataacaggc ataactgatt tgaataatcc ataagtacct actattatta tacctataac
    14281 aggtagtaca ctagttatat
//
LOCUS       DAEMHZ010000109        14173 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000109 DAEMHZ010000000
VERSION     DAEMHZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.109"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      complement(113..196)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(429..1271)
                     /locus_tag="KPK72_002856"
     CDS             complement(429..1271)
                     /locus_tag="KPK72_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2490054.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNTGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1523..3307)
                     /gene="pepF"
                     /locus_tag="KPK72_002857"
     CDS             complement(1523..3307)
                     /gene="pepF"
                     /locus_tag="KPK72_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2490055.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3373..4038)
                     /locus_tag="KPK72_002858"
     CDS             complement(3373..4038)
                     /locus_tag="KPK72_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2490056.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4189..4350)
                     /locus_tag="KPK72_002859"
     CDS             complement(4189..4350)
                     /locus_tag="KPK72_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG2490057.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4428..4937)
                     /locus_tag="KPK72_002860"
     CDS             complement(4428..4937)
                     /locus_tag="KPK72_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG2490058.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5143..5361
                     /locus_tag="KPK72_002861"
     CDS             5143..5361
                     /locus_tag="KPK72_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2490059.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5379..7325
                     /gene="feoB"
                     /locus_tag="KPK72_002862"
     CDS             5379..7325
                     /gene="feoB"
                     /locus_tag="KPK72_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2490060.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(7485..8540)
                     /locus_tag="KPK72_002863"
     CDS             complement(7485..8540)
                     /locus_tag="KPK72_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2490061.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8588..9388)
                     /locus_tag="KPK72_002864"
     CDS             complement(8588..9388)
                     /locus_tag="KPK72_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2490062.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9381..10172)
                     /locus_tag="KPK72_002865"
     CDS             complement(9381..10172)
                     /locus_tag="KPK72_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2490063.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(10212..10634)
                     /locus_tag="KPK72_002866"
     CDS             complement(10212..10634)
                     /locus_tag="KPK72_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG2490064.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(10650..11129)
                     /locus_tag="KPK72_002867"
     CDS             complement(10650..11129)
                     /locus_tag="KPK72_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2490065.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKSDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(11261..14017)
                     /locus_tag="KPK72_002868"
     CDS             complement(11261..14017)
                     /locus_tag="KPK72_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490066.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISVLEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4586 a         2463 c         1609 g         5515 t
ORIGIN      
        1 aagccaaaat ttcacctatc aataaaaatc ttaacgacta atactcttat tttcaaattt
       61 tgcaacattt ttgttaattt taaagcaaaa aaagactatg caatataaaa attatattca
      121 tagccggttg caccactaac tccatatatc tccagatgcc caataataag atatacttct
      181 cagtttctaa aatattttta ttaaaaatct tttatatatt ttcataaatt tcttttttat
      241 tctaacattg taatattatt ttgtcaatat ttccatattt ttagatataa gatactaaaa
      301 tacctatttt atctaaagat atttatattt ctacattcaa taatagacaa taccctgcaa
      361 gttaaagcat catttttatg agcttatctc acagggtatt atatgtttac tttatattat
      421 aattcatgtt attgattaac tttaataaat cctttttctg aaacaagtgc ttgtccttca
      481 tcacttaata tataatctat taaagctttc cctttttctg acaaaccatc ttctttatta
      541 actaatataa atggtcttga tattggatat ttattagcta ttacatttgc atcagttgct
      601 tcaacatcat ttatttttaa actatttact ttctcatcaa catatccgaa tgatatatat
      661 cctatagcat tttcatttcc ttcaactgta gtttttatat ttcctgaacc atcacttatt
      721 tgtgcatctt tagttagttc ttctgaatca aaccctacaa tttcttggaa tccatctcta
      781 gttcctgaac cttcttctct tgatactatt actattggtg catcatttcc tcctacttct
      841 ttccagttag taatctttcc tgtatatata cctttaactt ggtccattgt caggtttttt
      901 actgagtttg cagtattagt tatcaatgct ataccatcta ttgctatttt agtttccttt
      961 aatccagcac ttttttcttc atcttttaaa tctcttgaac tcattcctat ttcagatgtt
     1021 ccttctatgg cattttttat acctccagaa gaaccaatct gttgtacttc tacactaaca
     1081 ccttgatttt tttcttgaaa cttttctccg atagtttcca taagaggacc tattgaagtt
     1141 gaacctgaaa cagaaacttt gcttgaatct cctgaatctc ctgaacctcc tgtgttagag
     1201 ccacatccta ctgctaaagc tccaactaac attgtactag ctaaaagaac gcctattttc
     1261 tttttaaaca ttttacttac ccccgcttat attttaattt tttgttgtca tttttctata
     1321 ttttaataat acagattctt tgttaaatga atattagagc agggttaagt aaatgtaaat
     1381 attgttaaat acatgtaaaa acttaatttt atatgattta ttcttaatta gattaacttt
     1441 ttaaagttac tttaggcaga tataaacata atacaactca tattattaga agtcttcatt
     1501 gaagctatca acttatcaca tcctataact ctttttctag tttattaact aattcagcaa
     1561 atacattcaa ggcttcctct atagattctt ttttcgtcat atctacacca gcagatttta
     1621 attgttctag tgggtattct gaccctccac tttttaagaa gtttatatat ctatctactg
     1681 ctgtacttcc ttctgaaagt atctgttgac ttaaagcact tgcagcagaa aaccctgtgg
     1741 catacttata tacatagaaa tttgaataaa aatgagggat tctcgcccat tctattccta
     1801 tttgtttatc tactattgca gattttccgt aatacttttc atttaatttg tagtatatat
     1861 tagtaaattc atcagcagta agtggttctc cactctctac cctttgatgt acaatttttt
     1921 caaattcagc aaacatagtt tgtctatgaa ctgtagttct aaattgttct aaataataat
     1981 ttaataagta tatagtttca tccttagact tagaattttc taataaataa tttataagaa
     2041 gtaattcatt taatgtagat gcaacttctg caacgaatat cttatagtct gaatataaaa
     2101 atggttgact tgttcttgaa taatgactgt gaacagagtg ccctaattca tgaattaaag
     2161 tgaatagaga attaaggtca tttttatagc tcattaatat ataaggatgt gaatcataac
     2221 ttccccaaga ataagctcct ccttttttac cttcattatc atatacatca atccaccctt
     2281 catcaaaagc actttttatg accttcaagt attcttctcc taaaggttta agtgcctcta
     2341 atatgatatc ctgtgcttta tcatatggta tatccatgtc aaatttatct gtcaaaggaa
     2401 catacaaatc atacatgtgt atttcattca atcctaaaaa tttcttttta agctcaacat
     2461 atttatttag attaggaata cttttatcta tagctgatat aagattattg taaacatcta
     2521 aacttacatc atctgaaaat aaagacgctt ctattgctga attatacttt ctagtttttg
     2581 cgtaaaatat ctctgatttt attccaccat aaagcgttga ggcaaatgta tttttatatt
     2641 tctcatatgt tgaaaattca ccttcaaaag cgtcttttct aactctacta ttcttacttt
     2701 ttataaataa agagtagttt gaatgagaaa gcttaacctt attcccctcc tcatcttcta
     2761 tctcaggaaa ttccatatct gcatatgcta acatatcgta tacattttct ggaattgtcg
     2821 ataattctga tgtagctgca agtatttctt cctctcgttc actcaaagta tgtggtttat
     2881 ctctcaatat atcctttata tattttgtat aaaaggatag cttctcatct tttaagtatt
     2941 cacttaactt attctcttct atcgccagta tttctggaac tatataagaa gtagccacac
     3001 ctaaatcagt agataacatt tctgtcttag tggctcttcc ttgattatca ttaattctag
     3061 tatcttcatg ttgtttcata tgagtatata catataaatt ttgaacaatc ctagatgcat
     3121 tttcagagat atttaatact ttatataagt tctccttact ctcagaaagc ttccctttat
     3181 attcttttac ttcttggata agatttttta ctttacttat gtctttttca atttcctctt
     3241 tactggagta catcttatct atcttccatt tgtacttttc ttctattgta cttctatcag
     3301 ttcccatatc atacacctct attttaattt gttttatatt ttattcactt tacaaaatat
     3361 attgtatata ttttagtcca aataatctac atttattact ccttctatca tatttatttc
     3421 tttcataacg tcgtgtaagt gtattctctt agcataagat acaaaaaaat taaccttcca
     3481 gtattcttca ttacgacatt ctatttccat atttcttacg ataagttgat ttttttcaaa
     3541 tttatcatat aaaaaaggta tagagtccaa attgcttaat tttaatatca atacattctt
     3601 ttcttggcta aataaaaacg tattgattat cctaagcaca aataatgcta tcattacaaa
     3661 taatccacct agaattgcca attcataata accatatcca actgctatac caatacatgc
     3721 agttgcccac atcccagcag ctgtagtaag cccttttact ccacctttat tttttaaaat
     3781 tgctcctgca cctaaaaaac caattccaga taatacttga gcaggcattc ttgctgggtc
     3841 agcatttgta tacaaattat aatcaaagaa taattgaatc gaagttattg aaacaataca
     3901 agaacctatt gcaattagta tatgtgtcct aaatcctgca aactgtctta ttttttctct
     3961 ctctaagcct attattcctc ctattattag tgctaatagt aaccttattg ctatctcctt
     4021 acttcctata tcaatcataa tataaaaccc tcctgactta attacaactt atgtcctaca
     4081 gataatttac acatcagaaa tactatttta tatcttaata ttattattaa tatatgtaaa
     4141 atatagccat ctgttaattt ttgatacaaa aaaacccaca actgtgggct atttgcttag
     4201 gtacatgtat ttatttatta atatatctat tttcttactt gattctacta actcttcttg
     4261 aaatatttct ggatattcta tgtatgtatt tatctcttcc ctaactgctt caatctcatt
     4321 ttttaacgct tctaattcat tgttcgccat aataatcacc actattacaa atttgtatat
     4381 ttttatatac attttatcaa acaatattat attttgtcaa tattatgtca ataaaactta
     4441 ctataaaaat gctttaattt aggtattgta tacctggata ctaagcctac aaacatacca
     4501 gttactaatg atgccattaa cataaatggt aagtacccta tagcaagtat attttctatg
     4561 attaaacttg caactattaa ttgacctata ttatgcccta ttgcacctat aatgcttatc
     4621 cctattagtg ataaatcttt tattttactc gctaaataca taaataaaag actcagataa
     4681 gctcctccta tactaaatag tagatatgat aaagggcctg caaatattga tgacaaaatt
     4741 accctgatag taacaattat aacagtatct ttgacaccta acaacattaa tgaaattaaa
     4801 gttataatat tgctcaaacc caattttgcc cctggaaata taactataag aggatttggt
     4861 atatatgtct ctagtacata taaagctaaa ctatacccta tcataagacc taaaaatgtc
     4921 atttttctag ttttcattaa gttaactcct ccaaatttta tctatcatct tacattataa
     4981 tacaaaatat ataaaaacac ctaatttaga ctaaaatatt tttaatctat ttagaataaa
     5041 tattagttat ttcaatgtgc aattacctct ataaatcttg actttttgta tcatacttgt
     5101 tacaatttta atgataatga atttcattag gaggtgttaa taatgactgt ttacgattta
     5161 aaaataggag aaaaaggatt tattaatagt atagatggag atattaaatt atctaaaaga
     5221 ttgcttgcac ttggatgtat aaatagtact gaaatagaag ttaaaaaggt tgccccactt
     5281 ggagacccaa ttataattag atttagaggc tttgaccttg cgattagaaa atctgatgct
     5341 aaaaatattt ctttacaata atagaggagg agctacccgt gattaatgta gcattattag
     5401 gaaacccaaa tgttggtaaa actactgttt ttaatctact tacaggttca aatcaatatg
     5461 taggtaactg gcctggtgtt actatagaaa aaaaagaagg atttcttgga aaagagataa
     5521 aggttgtcga tttacctggt atatatgcaa tggatacttt ctcaaatgaa gaaaaggttt
     5581 ctaaatcata tttagaaaat gaagatgttg atgtaatagt aaatgtagtt gatgcttcta
     5641 atttatcaag aaacctttac ttaacaactc aacttatgca atttaacaag cctatagtta
     5701 tattgcttaa tatgttagac attgcagaat ctaaaggagt taatatagat gccaaaaaat
     5761 taagtgaaga acttggagta attgttgttc caattatagc aaaaaagaaa aatggtgttg
     5821 ataggataga agaagagatt aagggagcta cgcaaaaagc ttttatatat cagaaaaact
     5881 ttggaagtga aacagaaaca tataaaaaat tagaatccat tttatcgaga tgtacaaaaa
     5941 ctacatatac acaaaagaaa tctatcagtg ataagataga caatatagtc ttgaatccta
     6001 tattagcata tccgatattt attggtgtat tgttcctctt atttaaattt acctttgact
     6061 gggtcggagg accactacaa gagggatttg ctagtcttat agaggcttat atttctgcac
     6121 ctgttagtga tatgttatct aactctagcc catggtttaa atcacttata gttgatggaa
     6181 taattggagg tgttggtggt actttaccat tcttcccttt aatatttaca ttattctttg
     6241 gtatatcgtt gtttgaagat agtggataca tgtcaagaac agcattttta atggataaag
     6301 ttatgagaaa ggttggtcta tctggtaaag cttttatacc tatagttatg ggtatgggat
     6361 gttcttctcc agctataatg gcaactagaa cattggaaag cgaaaaagat agaagagtta
     6421 cagctcttat tgcaccactt atgacttgtg gagcaaagct acctatatat gcactatttg
     6481 tagctatatt ctttccacat aatgctgctt tagtaactac gtcactgtat ttggtgggta
     6541 tagttatggc aattgtagtt gcattattct taaataaaac agcctttaaa acagaggctg
     6601 aaccatttat attagaatta ccagaatata gaatccctac tatagatgca cttattaaaa
     6661 atacttggaa taaatcaaaa ggtttcttga taagagttgt tactgtcatg tttgcaatgt
     6721 cagtcgtaat ttggggactt tcttcattca atgtttttgg atatacagaa gatataaatg
     6781 taagcttctt ggctactcta ggtcatatta tatctcctat attcaaacct cttggttttg
     6841 atgattggag aacttcagtt gctattttaa gtggtcttgg agcaaaagaa atcgtagtaa
     6901 actctttaaa tatactatat ggtaatctaa ctgttgtctt accaacaata tttaatggag
     6961 ttactgcata tacattcttg atatttactg ctttatatac tccatgtata gctgcacttg
     7021 caactatgaa aaaagaatat ggcaataaaa tgatgtttac atcatttgca tatcaattta
     7081 tactagcatg gataatggct tttatagtta aaagtatagg tggagtacta tttatgggta
     7141 attcaataat ggaagctttg ataggtggaa taattatagt ccttgcactc tttattctgt
     7201 ttaataaatt caagtctaca aaaaacggaa atgtctgtag tggatgttct atttgctctg
     7261 gatgtccaag tgctaattct tgctcaagta aggaagacag caataaaaaa agtaatacac
     7321 actaatatat aaatatttta gtttaaaata agataaaagt gggtgtctca aaattaactt
     7381 tttagttcat gaggcactct tttttatata aaatcaaata tattttcatc atttcaacag
     7441 tcaaaaagag gcttatctct attttgagac aacctctttt tttgttactt attcagtttc
     7501 ttgctaccta acttcatgtg gaacctagta tcttttggaa cacttgggtc tacgcctctt
     7561 aatgtaggta tttccccaca tattagttgt ataggtacta taaaattaaa tgtttgatag
     7621 taaggattat ctgtgatatc aatctttaaa tttttatcat tttctgtaac atctctacct
     7681 atagcaaata cattttccgt ccaaccagaa agtacatcaa tcatcttagt tactctaggt
     7741 tcttttcctg tatctagtat aaatatagta gaatcactat ttatagcatt atatataccg
     7801 tgtataaact cttcaaactc atacccagtt acaggtattc tcattgtttc caataatttt
     7861 aatgctgctt ctaatgtatc tccatatata tcagagtgac ctattattct tatttcttta
     7921 gaattaacta acttttcttt gtttctttca atccattgtt tagagagctt atacactgct
     7981 tcaaatctat taattgcatc taatatctta tttatttcct cattgtactt ttcactagat
     8041 attgtacctt tttctctagc tatttgaaga cctaataaca ttaagtttaa ctttgtacaa
     8101 taatatcctt ttgttttagc tccagatttt tcttcaccac aatttactgt taatatataa
     8161 tcagaaatct catctattaa agcattctta caacctgcca tagatgctat tttacaaccc
     8221 ttatcttcag ctaacttcat agcgttgtat gtagaataac tactcccacc ttgagataca
     8281 cctactacta gggtattttc attatcaaac ttgaatgtat cttcagttat cataaatgga
     8341 tacattttca caacatctat atctaataaa ttttgtaaat atggttgcac ttgcactcct
     8401 gagtgatagc tagtacctga acctgttatt ataatctttt ttagatttgc tctcttaact
     8461 tcattaaata agctatctgc attagatata atttctctca ttctaactgg tgtttctaac
     8521 atataatctt gtattgtcat tttatacacc ttccttatct atatattcaa taaattataa
     8581 ttaatttcta taacaaacct atagcatgta aagcgattcc tgttactata gtgcctatag
     8641 ttaattttgt tacacttacc tttctcttca acatgtaata tattaaaaat gcaaaggtta
     8701 aaggtaacat attaggtatt attttatcta gtatatcttg taaaattact tctgcaccat
     8761 ttaagttaaa tttcaaagga gtagttatac tcaacatagt tgcaaccatt cctcctacaa
     8821 cgaataaacc gacagttgta gccatagaca ttatcttctc cataagtccc tctctttgta
     8881 ttctttcaag agaatttacc cctactttat aaccatactt aagtccaaat attctaaggg
     8941 caaatgctgg tatattgtat aataataaga ataatagtgg tccaaagata ttgccttctt
     9001 ttgcaagtga tactcctact cctgctgcta taatcctaaa tgttccccag aataatgaat
     9061 caccaattcc tgcaagaggt cccataagag ctgttttaac agcactaatt gaactttcat
     9121 caaattctgg attattagca ttttcttctt ccatagctgc tgttatacct aatatagcag
     9181 gaactaccac tgtcgttgta ttaaaaatct ccaaatgcct ttctattgct tttgcttgat
     9241 tttctttctt attatatagc ttctttattg caggtaacat tgaataacaa taacctaagt
     9301 tttgcattct ttcgtaatta aatgcacctt gaagagcaaa tgaacgccaa aacatacttc
     9361 ttaattcttt ttttgttaat ttattcaaag tcaatctctc cttccgttaa agtattcatt
     9421 gctaattgag gattttgatt attttctttg ttattgatat aaaagtttag tacaaatgct
     9481 acacatactc caaataaagc tatagcagtt atatctaatc caccataaga tgctaacaaa
     9541 aaccctataa agaaaaatac agacatcttt tttgaaagca acatatccat aagcaacgca
     9601 aaacctactg ctggcaacaa tgttcctgca acacccaatc cgtctaatat aactttaggt
     9661 attgcattta tcaatgttgt aactttatca gcaccaatca taattgctaa aaaggttgga
     9721 actgatgtac ttaaaaagaa taatattact ggcaaccaca tcattatggt aactttctta
     9781 aaatcagcct gtttagcata gaaaactgcc ttttgagaaa agtacgaatt tattattcta
     9841 actaatactc caagagattg tgctaatact gctactggca ctgccactgc taaagcaact
     9901 tctgcaccat tacctgataa tatagcaaaa gctgtaccca atacacctcc tgttactacg
     9961 tctggaggag tagctgcacc tatccctgca attcccatcc aaattaattc aagctgtgca
    10021 ccaatcataa ttcctgattt tatgtctcct aaaactaatc caactaacat acctgtaact
    10081 attggcctat caaacataga ttgaccaaat atacgcccat ctagtatacc aactcctgct
    10141 ataatagcta acatagtagc ttgtactaac atattgaacc tccattttaa cttagtttaa
    10201 tttacattaa attacattac atttttgatt ttatttcgat tttctaataa ctcatttaca
    10261 taaacaattc cattttttgc ttcctctaag cctgatttaa ctattagttt tgtatcctcc
    10321 tcaactaata agagtataga taaaagcatt ggtaaattta ctcctgtcaa caattcaaat
    10381 ttgaaccctt gcaaaagtaa gtttgtacat atatttgcca cacttccacc aaacacatct
    10441 gttaaaacaa caaattcttc actatcttta ttatttgata atatacttct aatttctttc
    10501 tcagcatcat ctgctgattt atcttttgtc atacaaaaat actcaatatt agctaatgaa
    10561 ccaattatta atttacttgt gtcaacaagt tctctagaca attctccatg tgttgcaatt
    10621 agaaattttt tcattctctt tctcctaaat tattttatta aatctgatag ttgtttcttt
    10681 ttgtctctag gtaacattct aagttctata tcaattccta aatcacctat ttctttaaag
    10741 ttatttatat catcatcatc aacagcaact aaatctgtaa gtttcttttt tccttcttca
    10801 tatcttaacc ctcctacatt tatttctttt aatactcctc ctgaattttt agctacaact
    10861 aaagcatctc tggtattttt tactaacact aatacattgc ttttctttga agaagcaaat
    10921 ttttctacta tttctccaga ttcctctacc ccccttatat ataaagttac attgctaggt
    10981 ttcgctaagt tcagtgacat agctttcatt tggtcacttt ttgcttcatc atttgcaact
    11041 agtattacat ttatacctag tgatgttgcc catccatatg caacctgacc atgtatcaat
    11101 ctgtcatcaa ttctcatcaa agataccatt ttccccaaat cctccatcat atattaaaat
    11161 ttttattgtt tatactctca tttatctata aaagcaaaaa gtatgccaaa tactggcata
    11221 cttttttaaa tttcattcac ttattttgta atcttgtatt ttactgtggt agaccataga
    11281 taatattata aatatatcct atctctgaaa ttggtatttg cacactataa ctcttttcta
    11341 gcacactaaa tgcatttttt attagtttta cttcactatg atggtttttg attaacaaat
    11401 ccatatcagt atactcttca atttcagttt gtctaactaa tctttcaatc atacaactta
    11461 agtgaacata taaagaaatc tttttatcat ttgaaagttt tttcttttga atattttcta
    11521 tagcattaat tccttcttct attctctcta ttatcttttt tgaatctaaa atagttagac
    11581 tatctattac tctaattaat gaaaaattct ttacaataga atcatttatg ttctttattt
    11641 tttcattttc tactactctg ctaaatattc taaaaacatc atcttctcct tttcctgata
    11701 ttatatcctc taataatata aaatctaccc cattcacatt tggattagca gttcctacta
    11761 tagccatcac atcatacatt tcaaaaactg gttctttatc tcccatagaa acaagactat
    11821 cataatcata aggtattatt ttgacatcaa ctatcccaat taaactttcg gaaagtagat
    11881 gttggatttt tatagcagta cctatccctg taaaacaaga tgatattata gcatattctt
    11941 tatttttttg aggataatac aattgtatat taggtttagt ttcttcatcc ataatttctg
    12001 caatctcttc aatgttttta ccttgcatta ataaatttcc tacttctagt acagaaatag
    12061 tggatatttg gtctataaat aacattgggc tatttatctt attctttaaa tcttcataga
    12121 taagattaag agagcccata tcaaataata caattaaacc attctttatc ctatctcctt
    12181 ctaggtatct agacatttgt tttgatacat cagcaatagt agcatctata ggcatatcaa
    12241 tagctgtaaa tatattttta cccaacatcc gattacatac attcgccata ctactggcag
    12301 tagcatatcc atgtgctata atagtcgcat taacttgatt aaatttcatt cttataattg
    12361 ttgatttaat aaatataatc attatgattg tatctacatt attaagatac atatccaatc
    12421 tattcgttat taatgttgat attttactta ttatagagta ttctttgtaa ctctcttttt
    12481 ttaaatatat tagtaattta ctttctaaag ctttatactc atcagataat tgataagatt
    12541 cgtccttcaa ataaagataa gacgaaaatg ccaatacatt attcccatcg tattttatat
    12601 tgttattaac ttctaaattt ctaaatatat cctgcattat cgatattata agctccataa
    12661 gtacgttatt attttcatca ttatttgaaa agactaaatt gtccattagt ctaatcatta
    12721 gattagaact ctttttacta aatttttcct ttgttatttc atttttttct agtctatcaa
    12781 agtagtctaa acattttatg aaactttgat agattatatt tcttttattt cttccatttg
    12841 acatttcaat actaaattgt ttaggagaaa agataatgct tttgctttta ccactttgga
    12901 attgaaatat atctacattt tctatataaa gatttctagg aaggtcttga agagtaacaa
    12961 aaatatttcc atttcttctt tgattttttg cataagctcg tccacatgca tattttatta
    13021 catttttaca ttcacctata ttccccttga attttatctt taaaatcgta tttaatgtaa
    13081 tttgagttat ctctataggc aagccaagta ccttactttc ttcataaata aaatgttcta
    13141 caatctttct tttttcaata gtccctcttt cttctatatt gggaacataa atatttatgg
    13201 gtattcttct taaaaaagtt tgcaaaaatt tagatagact ttctgttgtt gcaaatatta
    13261 atcttaccct agcttttcta attactccat tctctccaat tctagaaaag gtacctgtat
    13321 ccataaatgt aaataacttt tcctgacctt cactatttaa tctatgtact tcatctaaaa
    13381 ataaaacacc accatctgat tgttctatta gacctgctct agattcattt gctccagtat
    13441 atgctccttt tacatgccca aataatagac tagacaataa ctccaaatta ttataatact
    13501 gcgcacagtt taacacattg tatggagaat ttttttctaa tacattactg cttacactat
    13561 atttgtatat tatcttggct aaaaaagttt ttcctactcc actttcacct gtcagcatta
    13621 ttgtaagtcc gccatttgga taaagtacag cggtttttat ttgttcgatt acttctttta
    13681 agctattatc atatcctatg atactattaa acacagtctc ttgctttaat aatttacact
    13741 catttttaag ttcatctaaa ttttcataaa tacttttttt tgtaggtcct actacatcat
    13801 ttattttatt tttaggtaaa aaaataacag gtctactgtt tatctttata agattatcag
    13861 attttactaa atcatttaat aatctacttg attgagttct acttaaatta aactttttta
    13921 ctatatcttc tgttattatg ccactttctt ttttaagttc atcaaagttg ttaaataata
    13981 cacattcatt tacaaaactt aaaatttcat ccctcatgtt caacctccaa tacaaatatc
    14041 aataatacta taatattagt atatcattta tatatttatg aaaaagataa ttatattcct
    14101 atcttgattc tcttttgtaa ggttgtaaaa tcaaactttt tataagtata aaatctagat
    14161 tttttaacat aat
//
LOCUS       DAEMHZ010000110        14091 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000110 DAEMHZ010000000
VERSION     DAEMHZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.110"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(252..878)
                     /locus_tag="KPK72_002869"
     CDS             complement(252..878)
                     /locus_tag="KPK72_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2490067.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(954..1925)
                     /gene="nrdF"
                     /locus_tag="KPK72_002870"
     CDS             complement(954..1925)
                     /gene="nrdF"
                     /locus_tag="KPK72_002870"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG2490068.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(1938..4037)
                     /gene="nrdE"
                     /locus_tag="KPK72_002871"
     CDS             complement(1938..4037)
                     /gene="nrdE"
                     /locus_tag="KPK72_002871"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG2490069.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4515..5240
                     /locus_tag="KPK72_002872"
     CDS             4515..5240
                     /locus_tag="KPK72_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2490070.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKTDLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(5368..6564)
                     /locus_tag="KPK72_002873"
     CDS             complement(5368..6564)
                     /locus_tag="KPK72_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490071.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(6567..7592)
                     /locus_tag="KPK72_002874"
     CDS             complement(6567..7592)
                     /locus_tag="KPK72_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2490072.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(7664..8794)
                     /locus_tag="KPK72_002875"
     CDS             complement(7664..8794)
                     /locus_tag="KPK72_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2490073.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(9022..9203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9256..10149)
                     /locus_tag="KPK72_002876"
     CDS             complement(9256..10149)
                     /locus_tag="KPK72_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG2490074.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNKKQVCEIKKLMNELNIC"
     gene            complement(10229..11131)
                     /locus_tag="KPK72_002877"
     CDS             complement(10229..11131)
                     /locus_tag="KPK72_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2490075.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(11181..12341)
                     /locus_tag="KPK72_002878"
     CDS             complement(11181..12341)
                     /locus_tag="KPK72_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2490076.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(12497..12802)
                     /locus_tag="KPK72_002879"
     CDS             complement(12497..12802)
                     /locus_tag="KPK72_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2490077.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(12900..13874)
                     /locus_tag="KPK72_002880"
     CDS             complement(12900..13874)
                     /locus_tag="KPK72_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2490078.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         4517 a         2576 c         1524 g         5474 t
ORIGIN      
        1 tatatgtaat ttattttaat taataatgca tacatcttac taataatcta cttatataat
       61 aatctataaa tattatataa taatacttat ataattagta ttattattta ataacaacag
      121 ttattcacac taataaaagt agaaagtttt taaaatatta tgaaacatca aatttaattt
      181 taaaattgca tcaaaaaatg aagtctcaac agacttcact ttttataact cttataaatt
      241 attcaaaata tttatacttc cctttctact tcaaaacgta aaaccgtctt caatgattca
      301 ggcgatactt ttctagcatc acttatataa atttcacgat gtgtgtaaga ttttcttata
      361 agtttatttt ttttgcaaaa ttcgttcata atgtcaaagc tttctggttc attatcatat
      421 gaacctatat gaagcatttg aacacatagc ccttcattaa tagacccaaa tctaactttt
      481 tcaagaagat tatgaggttt tttcttcttt actatatcag tagctttatc caacaggtct
      541 tgtgtaacaa aatctggctg acgtatcatt atagtgtaaa ttaaatcttc tttaataagt
      601 acatctaagc ctttactttt ttctccctta tcccatatac cttctaaagg aaaaaccgta
      661 tagtcaaaat acccttctgg tacaattcct tttttaggca ttgtttttat tgcatatgcc
      721 aaagaataaa gtactcctat tgcttcagta aattctttag aatttgggtc tccactccct
      781 tttaacatga taaatttctg ttctggtatt tcaactatta caggattctt tttaggtaaa
      841 tataactctt tatctctttt tctccattca tatttcataa aaatacctcc atatatattt
      901 tatacattga tattatcacg ctaaaagtga caacagtgtg tcataattat aaattatatt
      961 ataaaatctt catcctctaa ttcctcatac actccttttt ggtatccatt acccttagtt
     1021 gaaaaaaaat catgtgtttt ggtttcagtg ctaagaccat ttaatacaat tggatttata
     1081 gcatctacag tatatagctt ctcaaaccct agattttcta aagctctatt cgcattgtat
     1141 tttaaaaagt tcacaacctc tttttcaagc ccactttctc tgtatataac atttgtatac
     1201 tctatctcat tctccataag gctataaagt atagaataca tcttctcttc taacatctcc
     1261 ttatccattt tatcaaatga atcgtatatt tcttgtgcta gcaatcctat atacttacca
     1321 tgtaaagact catctctaag aataagcgat attatttctc cactagctac catctttcct
     1381 tgaccagata gatacaatgg ataaaagaat cctgaataaa acaagaaact ctctaaaaat
     1441 acacttgtaa ccattgatag gtataatccc tcttgatttg tagtattttc gtattgagct
     1501 aaaactaaat cagcctttct ttgtaatgtt ggttctgttt ctatccattc aaataattca
     1561 tctatttcta aattagaaag tagagtcata aatatagaag aataactttt tgcatgtatt
     1621 tcttccatag tccccataaa agacaagaca gcttttcttt gtaggttttc tgttaaactc
     1681 atcatagatg gtattccatt attcccctgt tttgtatcca gtaatgttaa accacccaat
     1741 acttttttat ataattcctt ttctttatta cttagttcat tccatacctt tatgtcttta
     1801 gaaacagata tttcttcagg aagccaaaat tgttttacat tttgttccca aaaagcttgt
     1861 gtaaatccat cttcttctct attccagtta actgcattat gtattttatg taaattaaat
     1921 gtcatatatt tctctcccta tactgaacac actagacact catgagtatc tcttgtcatc
     1981 tttgttcttg tgtaatataa acttttcaaa ccttttttat aggcatatat gtaatgtcta
     2041 gctatatctc tagttgtctt ttcatctgta acaaacagtg ttgttgatat accttggtca
     2101 acatggtttt gaactgttgc cactaaatct atgacctttc tcatatccat cctgtatgct
     2161 gattgataat acaacatatt atcatttgtt aaaaatggca ttggatatat tgtagtagag
     2221 tctccatatg ttcttacttc tactggctca gtaattggca ttatacttga tgtagcattt
     2281 tgaacataac taatgctttg agttggtgct actgccatta aatatgcatt atatatccct
     2341 ttttctttta cttcatccaa taattttgtc cagtcttctt ttgttggtat ataaatccct
     2401 tcaaataatg ctcttacctt ttctgactta ggtagataag attgttcata gtattttgat
     2461 aaaaccttgc tatttcttcc cttaacatat tctgatttat caaatccttc aaaagtttga
     2521 tttctttcta tggctatttt catagaagct ttaattgaat agtatctaac cattgcaaaa
     2581 aatacattag aaaaatctat agcatcatct gaagtatata aaatattttc tcttactaag
     2641 tatccatgta aattcatagc tcctaatcct atagaatgag acttgctgtt actattgctt
     2701 accgttggca ctggttttat atctgtgcta tctgctacaa atgacagtgc tcttattgct
     2761 gtttcaactg tactttctat agttttatta tccattacat tggctatgtt taaagaacct
     2821 aggttacaac ttatatcttg tccccattca ttttttcctc catacccctc tatttctgaa
     2881 ggtgtttgat attgaaatat ctcacaacat aagttagaca ttttaataat tccaacatct
     2941 tttaatgtat gttctctatt agctgtatct atgtacacta catatggata tccactttct
     3001 tgttgcatct gtgctatttt tgtcaacatc tgcctaggat ttatttcttt ttttcttata
     3061 tcactatcat taaccaattt atcataccac tcatccattt ctatctcatc aagagaaact
     3121 ccatatttct tatacacagt atgaggataa aatgcatatg ctacttcatt tttttctgcc
     3181 aactccataa atttatctgg tattatagct cctaatgata atgtagcaag ccttattttt
     3241 tcatctgcat ttatcttttt agtatccatc aacaattcaa aatcaggatg gaatacagta
     3301 aggtacaccg caccagaacc ttgtctagct cccatttgat taaagtaact aaatgattgt
     3361 tctaacattt tagctactcc aacaacacct ccagctgcac cttctatatc tttgatagat
     3421 tcgccagatg ctctaagttt agataaattt agtgcaactc ctccaccaat ctttgataag
     3481 tgatttgatg aagatatagc atacgatata ccttctgtac tatcttctac agatagtaaa
     3541 aaacatgata ccatttctcc agcccttttt cttccagcat ttaaaaaagt aggtgtagct
     3601 ggttgaaatt cttgttttac tattttatct gccaagttta aagctaaatc tttattgcca
     3661 tttcctagtg tcaaagctac aattaacact ttatcattat atgtttctaa tatttccttt
     3721 ccatcattgc tctttaatgc ataattttga taaaacttat ttgcactcat atatgattga
     3781 aattcaaact tatgtttatc aatcatatca tatatatttt taataacatc tatgctatac
     3841 tgatttatta cttcttttga ataataacct tcatctatta aatacttcaa tctttcttct
     3901 aggttattaa attttctcaa ctttggctgt ataaattctt ctatgtaact acttaaggct
     3961 tccttatcct tttccaattg atacttgcct tgttcatttt taattatcac ttgattgtta
     4021 agctctatcc aattcattat ttaccgtcct tttaacataa tcttgtgcca taatttcact
     4081 taatttgctt attatttctt ttggtactta tcagttgaaa tcccaaaatt aatctataat
     4141 tatagaaatg caataagtat ttttcaaaaa caaaagcact acatatagtc aatgctttat
     4201 ctattatata ctacatatag tatatacaca aattttttta tatctaaact tttattactt
     4261 tataattgac attattttta tttaaagaaa tttttatcaa ctcaaatata aaacaataca
     4321 agcttattat gtcctgttta aaaatttaaa ttatctctaa atagcttaaa aatatattct
     4381 gatatattga aaaaagtact ttcaagatga tattttatat attatttaga agttttttat
     4441 ttattgttat ataattaaat atatgactat tttgctacta catatataaa aataaattta
     4501 tgaaggtgat tttaatgaaa actatttcta aacaagaatt agaaaaaaca cttgagaaac
     4561 attatttatg gcttaaaaac aataatgctg gagaaaaggc agatttaaaa gaattaagat
     4621 tagaaaatat ggacttaaga ggatactgct taagtaatat tgacttttcg tggtctgacc
     4681 ttatcaattt aaatttagaa aaaacagatt tagaaaactc tatttttaat aattcttatc
     4741 tttcggattg ttcattaaaa aattctatat taaaaaatgc agacttaagt ggtgcaaatt
     4801 ttagattttg tgacttatct aactcagata ttagaggagc taatttagaa aattccaatt
     4861 tagaacatgc tactttaaat ggagtaatat cagatgaaag tacccgtttt tttaaattgt
     4921 attgtcctga aacaggagca tttataggct ataaaaaatg ttttaatttt agaatggtac
     4981 aattattaat acccagcgac gcaaaaagag tgtctgctac atcaaatgct tgtagatgtg
     5041 ataaggcaaa agtactgtca attactagta tagatggtaa agaatctttt aaaagtgctc
     5101 gtgcatatgc tgatgaaaat tttatatata gagtaggaga aacgattgta gctgaagatt
     5161 ttaatgaaaa tagatggaaa gaatcgacta cagggattca ctttttttta actagagagg
     5221 aagctattgg ttatctataa aatgctaaag ttttataaat atataaatac ataaacaaaa
     5281 taacaaaaga gtatagattg taatttcaaa aaaattataa tctatactct tttttctata
     5341 acacataagt acattttatt tcataaatta taataaatta tttatatctg cactattgat
     5401 tatttgagaa actttatcta tatgttctat cttactatcc aaactgtcat aatacttctt
     5461 actctcatct ctatttctaa aagaataaac ttttttatcc aattttaaag cttcttttat
     5521 aatatctata ttcctcttat ttgtctctcc tacactaaat cctgtatcta ttataatctt
     5581 ggaatcattt aaatttctaa tagccaaatc gaaactctca tcacttatag gctcaaatgg
     5641 attttctgta aacatcttta tccccatagt tctaccaatt tcataatcaa tatcattttc
     5701 atgtattatt ccagaataca gacctatacc ttttttagta aaagctctat aaattggtgt
     5761 tgctttaccc ccaccaccaa ttataaaaac ttcattttta gatttattac taatttctac
     5821 tgaacctaaa agactattaa agtttttatc atccaattca taaagcgaat ttataatatc
     5881 ctcatctact acatcttcag gctggccata agctatgact ttattatttt ttataagagc
     5941 aactttatca caacttttta atgctatgtc tatttcatgc aatgaaagta ttactgatat
     6001 tgacttttcc ttacttaatt ttttcagtat gtttatgagt tccaatctat gttttatatc
     6061 caagtgagtt gtaggctcat ctaatataat tatttctggt tcttgaacaa gagcccttgc
     6121 aaccaacacc ttttgttttt ctccatcact tagttcatca aaatatcttt tctttaaatg
     6181 taaggcatct acagactcca atgcttcatc taccattatt aaatctttct tagatagact
     6241 cccaaaaaat cctgtatatg gataccttcc tatattgact atatcttgta cagtcattaa
     6301 atcacctaat aatctatttg taagtactaa agccattttt tttgataatg cttttcttga
     6361 tatacaattt atgtttacac cattcaaata tatttcacct tttattggtg atataagttt
     6421 agataaggac cttaataggg tagtttttcc agctccatta cttcccaaaa gacatagtat
     6481 ttctccattt tttacttcta catttatatt acttataaca actttgttat tgtatcctac
     6541 acttaaatta ttagttttca acatgtctaa gccctatcct tccttttcac caataacatt
     6601 actacaattg gagctcctaa taaagatgtt atcgtactta ttggtaattc tactggtgat
     6661 aaaattactc ttgatattaa atcacaaaga gctgtaacta tactgcctaa aaatattact
     6721 actggtatca aaactctatt atctgatgtt ttaaatatca tttttgaaat ttgtggcact
     6781 gcaagtccaa taaaccctat aggtcctgca aatgctgata ctattgctgc taatatacta
     6841 gatataaata ctatcaaaac cctaaaaaat cttatattta tgccaatact ttttgcatac
     6901 tcttctccta gtaaaaaagc atttaaaggt tttattatga atacagtcat caaaagacct
     6961 aatataccaa ctattataag tatttctacc ttttcccatg taaatccaga aaaacttccc
     7021 atagaccaca tagtaaattc ttttaattta tcactatttg caaatgattg taacacattt
     7081 gttataccac tacatacata tccggtcata attcctatta ttaaaagagt tgttatattc
     7141 tttaccttat ttgcaaataa aatgactata gacattacaa ttaaagctcc tataaaagct
     7201 gctacaaata tactcattga atttacactc tcaaatccca atgtgtatcc tcccaaaatc
     7261 atgagagcta ctgataaagt agaacctgaa gatactccta atatatacgg ttcaactatt
     7321 gggtttttaa aaaatatctg taataaaata cctgatactg ctaggcatgc tccacctacc
     7381 atggttgcaa gtactctagg gattcttata ttgtatatta ttttataatg ttctgtatta
     7441 tccatacctc ttaatacaac atctattact tctttaaatt caatttttac agagccaaat
     7501 gctacacaca ctaaaaaagt aagtatcata cttataatta atactataaa tattgttgta
     7561 ttccttattc tcattttatt gataaaatcc atttgtatac tcctcttagc aaagctatat
     7621 taactaaaat ctataaaacc tatctttttg tgtcctctct gtactattta tcataattta
     7681 tataatgttt gattttttca cctgtagtac ctgggtggaa catctcaact aaatctacta
     7741 ttaattcgtc cgtcttatct actgattgat aataatctgg agcaaacacc cataggttcc
     7801 cgttttttac aacatccaaa tcagccaata caggtgcact agattttata gaatctaaat
     7861 ttggtgcata atctgaactt gatgagtata caaatatatc tgcatctttt gcaacttcat
     7921 gcaattgttc aatacttact cgcccttcat ttgaatctat tgaagcacat gcatttattc
     7981 caccagccat ttttatcata ttatccacat atgaatcttt aggagctata taagcacttc
     8041 cttcatatac agatgcccat acaactttag gtttagcttt gttttttatt tttttactta
     8101 cttcttctac cttttgtaca gttttattta gttcactagt agccatatct tctttatcat
     8161 aaaatgcagc tattaaactt gtccactcca ttcttccaaa tggattttgc tctttatact
     8221 cattgtctac aacataatta agccctaatt cttttaattt gtcaataaat tttgtatcag
     8281 cctctgataa accactactc actatagtta agtcaggatt taagtctact attttttcat
     8341 aatctggtgc taaatttgaa ccaacgtaat gtatttttcc atctttcatt aaatctttaa
     8401 tttctttaat agtccagtac tgctcttctg ttgttacggc ttttatacta tctagttcat
     8461 ctattgttct cagagaacaa gcctgtgttg tagatgctat taatacattg tccacaggcg
     8521 ttctaactat attagcattt ttatattttt caggaatttt aatcttcttt gggattaaaa
     8581 tcagttctcg attatctcca tcagttactt ttttaattcc atcttctaaa tcttcaacac
     8641 taaatccact agcatatttt aattctaatt tcttaccagt actagcttgt ttattctctt
     8701 tgtcactttt actacaccct ataagacctc ctaccataaa aattaccata gcaattaaaa
     8761 aaaactgctt taaaacattt gatttaaatt tcatctcttt cactccaata tcttattttt
     8821 tatgagtggc taaaaaactt taataatata tgctttttaa agtatactta ttaaagttat
     8881 cctaaagtca tctttaataa gtataacctt ttaataaaat aaaaaacttc tcctaattta
     8941 ataaggagaa gttcatatat caactttaca aaaacgaatt gatacttcat tttattataa
     9001 attacaatct ccaccctaaa ttgaacttat ataatactgg gcaggtctcc tgactcacgg
     9061 cttttcctcc atcacccttc ccaaaatttt tataaatttc agtggatttt gatttcgtat
     9121 ccgtatacag tagcgggggc tgtagtgaat tttcatcact ttccctatta aactaatata
     9181 gtaccctagt attattttaa tttaattttt attaagataa ttgtaattta ttgttgacct
     9241 ctttgcaagg ttgatttaac atatatttaa ttcattcatc aattttttaa tttcacatac
     9301 ttgtttttta tttggtttta caaatggagc agtcatataa tcacttattt gtaaccctct
     9361 gatattcata gcttttttca ttgctggtat aaatggattg gttattaagt aaaaatccat
     9421 tgctttatta atatattttt gaatttctat aaccttctcc atatctctat tattgtaagc
     9481 ttcagcccat ttggagcaaa cttctggaat taaatttgat aaccccccta tacaacctgc
     9541 tcccccagac attagattat gtataaagtt ttcatcataa ccactataaa ccttaaattc
     9601 tgggaactct attttcatag tatttattaa atctctggta tgtcccatca aagttactgt
     9661 atccttatat ccaactatgt ttttatgttt tcttgcaagt cttaatgtaa gttctggact
     9721 taaatcataa ccagttctat ctggataatt gtacaaatat atttcagcct ctgtattctt
     9781 agctatttca ctataaaata actcaatact atcgtcatca agagagaaat aataagggct
     9841 tattatcata acaccatctg ctccactatt atgtgcatag tttgaaagtt ctatagattc
     9901 atttatacac atacgacttg tacctataaa cacttttact ctcttattta catgactagt
     9961 tactaaatct ataagttctt tttgcatatc catagacata ttaaagaact ctccagtact
    10021 tcccattatg actagtccat ctacacctcc atctataaga tggtcataca cttttttatt
    10081 accttcttta tctaaatttc cttcttcatc aaatattgta acaactggtg tcaaaaattc
    10141 tgctttcata atttaaactc ctaaaattta ttttaatatg ttcattaata catcagattt
    10201 agctttaata ttaattatat taaaacagtt acttaatgta attttttaaa attccaatac
    10261 tctcaatctc acattcaact acatctccac tactcatata tcgaggaggt gaaaatccca
    10321 tccctactcc agaaggtgtt ccagtagcta taatgtctcc agcctctaaa gtcatacctt
    10381 ttgaaagttc tgaaactatg tctgaaacgt ctgatatgaa taatcctgta ttagaacttt
    10441 gtctttcttc accatttacc ctactacata ctttaagttt tatgggaaaa ggaactgcac
    10501 ttttatgtaa aatacatgga cctaaagagg taaatgtatc taatcctttt cctctaaacc
    10561 attgaacatg ttctttttgc aactttctag cagaaatatc atttataaca gtgtaaccaa
    10621 agatataatc ttctacctct tcttttctaa tattaacacc tgttttacct attactactc
    10681 caagttcaac ttcatagtct agttgattat ttatctcaaa atgaccatct atctcatctt
    10741 ctggacctat tgcctttgtc acacgttttg taaaataaac cgttttttgt ggttcttcaa
    10801 aactatcatc aaagtgagtt tgagtttctt caagatggtc tttatagttc actcccacac
    10861 ataagatatc atgtattggt ttttctatag gaacatgtac agtacatctc tctcttttaa
    10921 aaacatcata attatagaat gtactttcat ttataatagt tttcaactta ataatatctt
    10981 cactagatac attttgaatt aattcattta aatccttaaa atttctactg agatttaaag
    11041 agttaacatc tataataaat aattcatctt tagaaaaaac tccagttctt ttacattcat
    11101 tttctccttc agtaaaactt acaaacttca taatctcacc ctttattaaa attctatgta
    11161 tttattttct tacttaaaga ttatacatag ttgcatactc tagctattgc catttcagta
    11221 tatccaaggg actctgcttt tgattgtttt ccatttgcta catctacaac catatttaat
    11281 aaatcatctg ttaattcttc cattgtttgt ggaccataga taactggact agcatcaaaa
    11341 tccatattat cttccatatt tgcaaaagtt attttatttc cagttatttt tattactggt
    11401 gctattggat ttcctgatgg tgttcctctt cctgtactaa ataccactat ttgtgctcct
    11461 cctgctacca taccagcaac agatgaagca tcatttccag gggtatccat tataacaaga
    11521 cctttcttat caatttgttt ggcataatca tatacttcac ttatttgtct atgaccacct
    11581 ttatgtatac aaccaagtga tttttcttct aaacttgtta aaccaccagc tatatttcca
    11641 ggagatggat taccttctct aacttcttct ccaaccaatt ttaatgaatt ttcatatcta
    11701 tgtactatat gtaatatttt ttcttttact tcttcattta ctgctctcct agctagtata
    11761 tgctctgcac ctataaattc agtagtttca cttaaaattg atgttgctcc taaatcgaca
    11821 agtttatcac ttaattcacc tattaatgga tttgctgcta atccacttgt tgggtcagac
    11881 ccaccacatt cagttcctag tataagttca gatattggaa attcttcttt tctaagcaaa
    11941 gaagcttctt gtgccatttc tctagcataa cgaacagctt tttctatagt ttttaaagtt
    12001 ccatgttctt cttgtattac aagagttttt attggcttat ttgttctctc ttttattgca
    12061 tcaactacca aatcattttg gcaattttcg catcccaaag atacaactat tacaccatat
    12121 acatttggat ttgctgcata accagccata acatccatag ttaattgttg gtctgaatgt
    12181 acttgtgaac atccattctg attattaaag gtaactgccc cttgaacttg tgatgctatt
    12241 attctagttg tatctgatgc acacacactc gtaggcaaga ttaatatgtg gtttcttata
    12301 ccaactcttc catctggtct tttatatcca taaaatttca tactgatttc ctcctaaaat
    12361 agtctatata ctttctataa tttttatata ttataatttt tattacactc actaatcaaa
    12421 cagctaaaaa cctatttaaa aactatttga cactaaaagc aatgaataca acttttataa
    12481 ctctataaat attattttat atattagtca taacttgttt atccaaatcc tctctaacac
    12541 ttctaacatt atgaatatgt acatgttgac cactttttat ttcctgattt gcttctccta
    12601 tatgttcacc atatttagta actttatcac ctttggccat atcttttata gccaatttat
    12661 gataaatagt tatatcatct agtgctgtaa tcgtttttac tgttttgtct tttaatttaa
    12721 aactaatttc actaccttta gctattgctt caatagctac tgcaacatta tctttttcat
    12781 caattatcac tgcatttatc attattcttg ctctccttta taaataatat aattttatta
    12841 atatcctaaa atctaattaa agaatttatt attttaccaa caactcactg taatataaat
    12901 taagctgtta ttgtaccatc ctcattttgt tttgggaact taggacatcc atacttctta
    12961 gcccaccaac tagttataag aggtactaat attgcagtga caactactgc ggctgccact
    13021 tgtgttgtag ctgttgcaac atatggttgc catgatgggt caactaatgc aacagcagca
    13081 ggtacagcaa ttgcatttcc agcagttgta gctacagccc atcctgcata tcctggacgt
    13141 tttcctataa atttatcaca gaatactata aatatacctc ctacaaatac agatattaat
    13201 ccaagtaata ctcctgataa acctcctttt agtatacttg caagatttat gctactacca
    13261 agtgcaaacc ctacaaaagg tacgagtaaa ttacaccctg gctctaaaaa ctctttcatt
    13321 tttttgtcaa aatttcctat tatcatacca acaagtattg gcactattgc agccaataat
    13381 gacaacaatg gtacatttgc aagacctgaa gtcccaagag ctattaatgt aaaaaatggt
    13441 ccatcattta aagtaagtaa actcatagtt gcacaatctg tttcatctcc atatgaagtc
    13501 atcaatgaaa gatatatact accattacta tttgtaactg cacttataac agccaatgtt
    13561 gtaagaccaa ggacaccatc cattccaaat agctttccta taagtatacc tatcaaagca
    13621 cctatagcaa atttagaacc aagaagtata ccacctctct ttaaaacttt tggcatttct
    13681 ctaaattgaa gctgagttcc caagcatacc agttgaaccc caattatagt tgcagaccca
    13741 acacttgaaa atactgctgt cgtaaacgaa cctatttgta caatatctgg gcaaaaggta
    13801 ttaatgaaag ctgctataaa cattggcact atcatcatac ctgcaggaat ttttttaata
    13861 aaatccatta tcataaagtt aatcctccta aaacatgata tttaattact cttattaagt
    13921 ttttagcttt aattctaacg gccgtttcgg ttcgtattta agaactaggt ttttaattac
    13981 gaatctttga attaatgata gcattttcct atttatgagt caataaaaaa aataaatttt
    14041 tgtcaaaatt ttttcaaact ttctgataat accttgtata ttatcaaatg a
//
LOCUS       DAEMHZ010000111        14068 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000111 DAEMHZ010000000
VERSION     DAEMHZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.111"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..447
                     /locus_tag="KPK72_002881"
     CDS             85..447
                     /locus_tag="KPK72_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG2490079.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            883..1584
                     /locus_tag="KPK72_002882"
     CDS             883..1584
                     /locus_tag="KPK72_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG2490080.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1855..2673
                     /gene="larE"
                     /locus_tag="KPK72_002883"
     CDS             1855..2673
                     /gene="larE"
                     /locus_tag="KPK72_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG2490081.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2728..3876
                     /locus_tag="KPK72_002884"
     CDS             2728..3876
                     /locus_tag="KPK72_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG2490082.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRILDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4012..5400
                     /locus_tag="KPK72_002885"
     CDS             4012..5400
                     /locus_tag="KPK72_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2490083.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5429..6127
                     /locus_tag="KPK72_002886"
     CDS             5429..6127
                     /locus_tag="KPK72_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490084.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6130..7563
                     /locus_tag="KPK72_002887"
     CDS             6130..7563
                     /locus_tag="KPK72_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2490085.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIKSEKNVGTKITFNIKNKFAKQA"
     gene            7585..8322
                     /gene="truA"
                     /locus_tag="KPK72_002888"
     CDS             7585..8322
                     /gene="truA"
                     /locus_tag="KPK72_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2490086.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8433..8640
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8893..10092
                     /locus_tag="KPK72_002889"
     CDS             8893..10092
                     /locus_tag="KPK72_002889"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG2490087.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10339..11385
                     /locus_tag="KPK72_002890"
     CDS             10339..11385
                     /locus_tag="KPK72_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG2490088.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLLLLFSE
                     VLEHMKTGYLILDV"
     gene            11493..12656
                     /locus_tag="KPK72_002891"
     CDS             11493..12656
                     /locus_tag="KPK72_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2490089.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRNRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPTWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12745..13125)
                     /locus_tag="KPK72_002892"
     CDS             complement(12745..13125)
                     /locus_tag="KPK72_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG2490090.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKIDVNYIF"
     gene            complement(13128..13787)
                     /locus_tag="KPK72_002893"
     CDS             complement(13128..13787)
                     /locus_tag="KPK72_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG2490091.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5664 a         1377 c         2396 g         4631 t
ORIGIN      
        1 gcttataatg attatgatat aaattggtta tagtatacaa tttatgggta taacaataaa
       61 aaattagaaa ggaattttta taaaatgatt aaattatatg gatatacaaa atgctctact
      121 gttaaaaaag ctaaaaattg gcttaaggaa aataatttag aatttgaaga tatagatatg
      181 gttcaaaacc ctcctagtaa agaggagtta aaatcaattt ataagactag tggatatgat
      241 ataaagaaat tttttaatac aagtggtatg aaatatagag aattgggtct taaagatatt
      301 gtaaaaactg aatctgatga taaactttta gaaatattgg taagtgatgg tatgcttatt
      361 aaaagaccat tacttttgga tggtaaaaat gtattattgg gctttaaaga agatgtatgg
      421 aaaagtactt tgctaaaaga agattaggaa gagataggtg tgttgattaa aattattaat
      481 taatacacct ataaatttat gtattaaatc taatctttat aaatattaat tagcaaattc
      541 aaatcaaaaa aattcatatc aacaaacata ttacttctta ttacaaatta tatcatatgt
      601 tttatattaa aatattataa tattacaacg aattgttaca aaattactta ttattaacca
      661 tgaacatttg ttttgtgcta tcatatttat ataagggaat tgtcataaaa aatatcttgt
      721 tcatacagtt ttatatagaa aaaagaaatg aatcttttct ttatataagt atattttgta
      781 aattgcctct atatttatag tttaaataat agtggtatta aaaatctgac aacaaaaata
      841 aaactttcaa aaaattaaat ctaaaaagaa taggagataa atatgagtat tgaagattta
      901 tatgattttg aatgtgttcc tttgaacaat gagattgata ttgatgtttt atttgaaaag
      961 atgatagaag atggaaaaga aaaagaatat acaccaatta tagtcattga tgaaaaagta
     1021 gggctattaa gaaaaaatat agatatgatt actaaagagt atggttctct tgaaaactat
     1081 agagagtatt gtttaacaaa atataatgaa attgatgtaa atcaattttt tgattttagg
     1141 aagagtgaga tttcagaatt attaaaggag ctaattgatt tagaggatga tttttatgat
     1201 tataaacatt ttagaccaat tatgaatata gatatatttc atgaatataa caaagtttac
     1261 atagctaaaa ttccaacaat taaaccatat gaagtatttg catacatacc tattggcgga
     1321 tttaatgatt gtcctagaga tgaagaacat atagctattg caaaatattg gtatgaaaag
     1381 catggaatct ttccaatcgc aattggatgt gatacagttc aatattctgt aaaaaattta
     1441 actaaagatg gtaaaaaatt tgatgactta tgtattgaat tagtatttta ttgtgaggat
     1501 attattatac aagggtatga aaccttaaag gctcttaagg atgtattaaa acgctctaca
     1561 atagtgcttt tttggtggga ttgatattct atcatttaga tagtattaaa ttgttagtca
     1621 ccaagaatcc acttgcttta gttatgggga gtgtgtcaaa ctaggttttt atacacagtg
     1681 tagcatagta atatgtttaa attacaaagg ttaaaaaaat actttaatat ttttaatatt
     1741 ataattaata gatttaaagg taattataat ttataaaata aatactttat atgttataat
     1801 cattaagaat gtaaaaaaat aatatatagt tttatagttc atatttttcc agatatggag
     1861 gtaaattttg tgaaggaaaa agaaaaatta gataaattaa aaaaaatgtt acttgaatta
     1921 ggcagtgttg tagttgctta ttctggtgga gtagatagta attttttgct taaggtcgca
     1981 aaagatacat taggtgaaaa tgttatagct gtgactattc atgcaatgat gcattcaagt
     2041 agggaaattg aagaggctaa acaatatact caaaattttg gtgttaaaca tatcatatta
     2101 aatatagaaa actttgattt aaaagaattt aaagaaaatg gtgttgatag atgttatcat
     2161 tgtaaaaaat atatattctc taaaataaaa gaggtagcaa aagaacataa tataaagtac
     2221 atagtagatg gaacaaatat agatgattta ggagactata gacctgggct taaggcatta
     2281 agtgaattag gagtaattag tccattaaaa gatagtagtt taaaaaaaga agaaattagg
     2341 tcgctttcaa aaacattagg attaaatact tttaataaac catcatttgc atgtcttgcg
     2401 agtaggatac cttatggagt tgaaataaca gatgaaaatt taagaatagt agaaaagagt
     2461 gaagaatatc tatccaattt aggtttttct caatttagag tgagaatgca tggagatata
     2521 gctagaatag aagttggaca ggaagaacta ggtaaatttt ttgaaaataa caattttaat
     2581 aaagttgata ctaaattaaa aatatttggc tttaaatatg ttacactaga tatgtcagga
     2641 tataaaatgg gaagtatgaa tttaaatgtg tagattattt taaaaataaa taagttttat
     2701 agttaatata aggaaacagg aggttttatg aaaaattata cgttaatatc gccatgtttt
     2761 tttggaatgg aaaaaatgct ggctagagag attactaatt taggatatga aataataaaa
     2821 acagaagatg gaagaataac atataaaact gatgagtttg gaatagcaaa gtcaaacatg
     2881 tggttgagat gtgctgaaag agtacatctt aaaattgctg aatttgaggc aaaaagcttt
     2941 gatgaattat ttgaaaatac aaaaagaata aactggtcta gatatatacc ttatggagct
     3001 caatttccta tatcaaaagc ttcctctata aaatcaaaat tatacagtac accagatgta
     3061 caagccatag taaaaaaggc tatagtagaa agcttaaaga aaagttattt ggaggatggt
     3121 ttgcttaaag aagataagga gaaataccct atttttgtat ttatacacaa agataaagta
     3181 accatttcta tagatacaac aggagatgct ttgcataaga gaggatatag agaaaaggca
     3241 aataaagctc caataagaga gactttagct gctgggctta tttatttaac tccctggaaa
     3301 gcaggtagag ttttagtaga ccctatgtgt ggttctggta ctatattgat tgaagctgct
     3361 atgattggta taaatatggc acctggctta aatagagagt ttatatcaga aaagtggaga
     3421 attcttgata agaaaatatg gtgggatgta agaaaagatg cttttaataa gatagataat
     3481 gaatctaaat ttaagattta tggatatgat atagatgaag aatctataga tatagctaga
     3541 gaaaatgctg aaatagcagg tgtagatgaa tatatagagt ttaatgtagg agatgcaact
     3601 caatttaaaa gtgaagatga gtttggattt ataatcacta atcctcctta cggagaaaga
     3661 ttagaagata aagacagtgt taaacagttg tataaggagc ttggttatgc atttagaaaa
     3721 ttaaaaaatt ggtcgtatta tttgataact tcttatgaag actttgaata tgagtttggg
     3781 caaaaagcag acaaaaaaag aaaattgtat aatggtatgt taaaaactaa ctttttccaa
     3841 tatccgggtc ctaagcctcc tagaaataat aaataagttt tacatgattt atttgtaaaa
     3901 tataaaattt tattaaaata agtaaaacat tatgatgcct tgaaacgtct aattattgtg
     3961 aggagatttt tgtgaacaaa tttaaagatt tataggatta ggaggagtta aatgaaagtg
     4021 ttaaagctta ttactgtagt agccctgatg atgttatttg ttactggatg taatataaaa
     4081 actgaatctc cagaacaatt agcgaagata cctgactatg atgatacaaa aaaagtattg
     4141 tatgatggta ttgaccagtt actaaaacca gattcatcta tgatattgcc ttctaataca
     4201 aaagaagtag gaaaaataaa taaggtagat ttaaacagcg atggaattga tgaattagta
     4261 gtatttgaac aaaaagaaga tttaagtaat aatgtgagtc aggtagggtt tgtaaccttg
     4321 agttacaatg gagaaaaata cgtacttgga gaccattttt tagaaaatgg cgaatcaata
     4381 gaatatgcta atttttatga tttggactca gatggatata aggaagttat cttattggtt
     4441 aaatccaaag acaaaacgaa tatgcatata tataaagtag tagataatga aataacaaaa
     4501 atatatgact tggatgcatc atggcttcaa aataaagaag attttaatga tatgaaagta
     4561 aaaattggat atatagatgg agatgacaag ttagacatat tgatgcttca tttgaataat
     4621 aagactaatg aaatgtttgc aagtgttgca aattttgatg ggaaaatgaa aatcaaagat
     4681 tctgttaagt ttgaaaatgt aaaaaactta agtgaattat atataacatt aggtaatgtt
     4741 gcttcatctg ttggaaattc ttctgcagct ataaaaggta ttgtacttga tattcctata
     4801 ttgaaagata ataactatat tacgcagata ctttatatga aagatgggaa aattaacaaa
     4861 gcttttagtg attatgataa aactattact aaatcttatt atatacctgt agatgatatt
     4921 gacaaagata agattataga aatacctata gttgcaggca gtacaggtaa taataaaaat
     4981 acttatagtt ctaaatcatc tgcaactata agttggtata gatggaatgg aaaagaaggt
     5041 gctgactcaa gtttaatatt tacaagtcag atatattata attataaata taattttaaa
     5101 ttatttattc caaataattt atttgataaa atacttgttg aacaggaatt tgttggagaa
     5161 aaagcagtat ttaaatttta ctattttgac tattcagacc gtaagaattt atttaatata
     5221 gtagtagaga gtaaaaataa acttgaagat agaaaaaata caggtactca aaattctatt
     5281 gtacttcaag agagcgatga atatactttc ttactagttg taaacaatgc aaatgaaatg
     5341 gataagctag atatgactat agacgcatta aaagaatatt tctcattaat atatggttaa
     5401 tattatactc aattggaggt tttattttat gaaggaaaag atacttatac tagaagatga
     5461 aattggtatt agaagttttg ttagtataaa cttaaaaagg gaaggctatg aaatagttga
     5521 ggcaggtact ggaagagaag ctatagaaaa aatgacaaca gaaaaggata ttacaatagc
     5581 tcttttagat gtaatgcttc cagatataag tggtatagaa gtatgtaaat ttataaggga
     5641 gaattttgac caagtaggta taataatgct tactgctaaa gctcaagaag atgataaaat
     5701 agaaggattt atctcaggag cagatgatta catcataaaa ccatttagta taaaagagct
     5761 tttagtcaga gtttctgctc ttcttagaag agtagcaaag gatgattcaa gtgttaagtc
     5821 gagtgagata gtttctccac catttatact tgatatagac aaaagaaaat tatttaaaaa
     5881 tggaaaggaa attgagctta caccaactga attttcaata gtgaaatatc taatatcaaa
     5941 tgctaagcag tcgttaagta gagaccagat attagacgaa gtatggggaa ctaactattt
     6001 atatgatttt aaaatagtag atgtaaatat aagaaggata agaaataaaa tagaagatga
     6061 cccatcaaaa ccaaaatata tacaaactat ttggggttat ggatactgtt ttagaaagga
     6121 agaataatag tgttacattt tgaaggtctg agaaaaaaag taataaaaaa ctattttata
     6181 ataattatta taatggttac tctgtttgaa ggattgttca tgttttacat acaaaactac
     6241 tattatgatt ctgtcaaaca gctacttgaa tctgaaataa aatatgctga tgaatataat
     6301 gctattacaa tggaaactac aagttttgag aaaaaagtaa aaaatatatt tgataaacaa
     6361 cccttaacta aaaattcaga atttggaatt tcaataatag ataaagataa aaatattata
     6421 ttagaccaat atggttttaa aagtaaagaa aaagcaaact atgaagatgt taacaatgca
     6481 ttaaaagata taaaaactaa aaatttaaca ccttatactt atagaatacc agatacaggg
     6541 gagcatgtta tgagtatctc cttaccactt aaggtaaata atataataga aggtgttgtt
     6601 agatatacag tatcattaga tgctattgat aatgcaatat taaagcaagc tacatggctt
     6661 atattagcag gaatatttat acttattata gctatactaa taagtcttaa atttgctgag
     6721 actcttataa aaccattacg agagctaaag aaatttgcaa atgaattggc tgtaggaaat
     6781 tataatataa aattagaaaa gatgaagatt gtagatgatg aaattggtga tttagcacag
     6841 acatttgaac acatggcaca tgagattgac aaaagtgaaa aattaaaaga ggaatttata
     6901 tcatctgtat ctcatgaact aaggactcct cttacatcta taaaggggtg gagtgaaacc
     6961 ttaggttatg aaagtataac aagagaagaa ctagacttag gtttaggcat tatacaagat
     7021 gaaacagaaa gacttataaa actagttgaa gaattattag acttttcaag gctttcttca
     7081 gacaggataa aacttcatgt tgatatagtt gatgttgaag gtttaattgt aggtgttgta
     7141 aatcaactta aagttaaagc agctgaaaaa gatatatcac ttttatttga atttgaaaat
     7201 gaatttatag agaacattca aggagataaa aatagattaa gacaggttct cataaattta
     7261 atacaaaatt catttaaatt tacgagtcaa ggtggttaca taaaagttgt agcttctcaa
     7321 gatgaagaga taacaaccat atcagtagag gataatggtt caggtataga aaagcaaaat
     7381 ttaaataaag tgttagataa atttttccaa gaagattata ataaggctgg tagtggatta
     7441 ggactagcaa ttagtaatga aattgtaaaa cttcatggtg gaagaatgaa aataaaaagt
     7501 gaaaagaatg ttggaacaaa aataactttt aatataaaaa ataaatttgc taaacaagca
     7561 taaattctta gaaagaggat taagatgaga aacataaaaa tgatagtagc atacgatggt
     7621 tcaagatata aaggatatca aaaattaggt gacaataata tgactattca agaaaaactt
     7681 gaaaatgttt taagtaaaat gactaatgaa acagtggaga taataggttc tggaagaact
     7741 gatatgggag ctcatgcaag aggacaagtg gtaaatttta gaactaattg tatggattct
     7801 ttggataaaa ttcaaaaata tttatatgaa tatttaccag aagatatagt tgtaaaaact
     7861 gttgaagaag ttgatgaaag atttcattca agatacaatg taaaatcaaa gacttatatg
     7921 tataaaattg acaataataa gtatcataat ccttttataa gaaaatatgc tactcatgta
     7981 agtaaaaaat tagatttaga tagaatgaga aaagcaagtg agtatttagt aggagaacat
     8041 gattttacta gttttgcttc ttctaaatct aagaaaaaat caaatgtaag agaaatttat
     8101 tctataaata taaaagaaga tgataatgtt atagaaatat atgtagaagg aaatggattt
     8161 ttgtacaaca tggtaaggat aatagtaggt gctttgatag atgttggtct aaaaagaaga
     8221 gctccgcaag atattaagta tatgcttgaa tcaaaagata gatgtcaatc atcagatact
     8281 gcaccagcta aaggactatg tctttggaaa gtaacatatt aatcaattat ataaaactct
     8341 ctttaaaata aaaataaaat aaattatata gttgacataa atggaaaaat aaactattat
     8401 atattttata agagagatat aaaactttat atttaaatcc tgtgagaaag agagtaactt
     8461 ctatgtgttt tatagagagc tgaggatggt ggaagcttgg caaataagta ttagtgaaaa
     8521 gaactttgga ggatactaat tgaatttagt atgttagtag gtaatctcgc cttaagagaa
     8581 agagtggata agtttatctt atcaattaga gtggtaacgc ggatatattt cgtctcttgg
     8641 tttttccaag aggcgttttt taatatattt aattattagt gtttatctgg tatgttgaac
     8701 atttactaat tgattagcta agtttatcaa taagagtggt accgcggata tttcgcctct
     8761 tagtatttta aaatactaag gggttttttt agtattaaaa tgattttatg tgagtctaaa
     8821 ttctcagagt cattcaatat taagaatttc tataaaataa aaattataat ttaagattgg
     8881 gaggaattaa aaatgaagga aaaagtagta ttagcatatt caggaggact tgacacatca
     8941 atcataatac cttggttaaa ggaaaattat gaagatatag atgttatagc tgtttgtgga
     9001 aatgtaggtc aggaagataa aatggaagat gtatatgaaa aagctttaca aagtggagca
     9061 tcaaaggcat atgtggatga tataagtgaa gaatttgtaa ctgaaactat atttaaagcc
     9121 gtaaaagcag aggctaaata tgaaggaaaa tacttattag gaacttcttt agcaagacct
     9181 ataatagcta aaaaattagt tgaagttgct cataaagaag gtgcaaaata tatatgccat
     9241 ggatgtacag gaaaaggtaa tgaccaagtt agatttgaag ctacaattgc agctcttgac
     9301 ccaactataa aagttattgc accttggaga atttgggata taaaatcaag agaagatgct
     9361 atagactatg ctgaaaaaca taatataaag gtaacagcaa ctaaagctaa aatatattca
     9421 gtagatgcta acctttggca tgtttcaaca gaaggtggag atatagagca cctagaaaat
     9481 gaacataaaa aagatgttta taaacaatgt gtagacccag aagatgcatg tgatgtagct
     9541 gaatatgtag aagtttactt tgaaaaaggt gtacctaaaa aggtaaatgg cgaagaatta
     9601 tctccagttg cactgattca taagttaaat gagttgggtt gtaaacatgg aattggtgtt
     9661 atagatatag ttgagaatag acttgttggt atgaaatcaa gaggtatata tgagactcca
     9721 ggaggaacaa tcctttatga agcacataat attttagaaa gtgctacttt agacaaagat
     9781 acattacatt tcaaacaaat ggtatcttat aaatacggag aacttattta caatggacta
     9841 tggtattgta aattaagaga atctatagat gcatttatgg aacaaactca agataatgtt
     9901 acaggaacag taaaagttaa actttacaaa ggaaatataa aacctgctgg aatatttact
     9961 gaaaatgctc tttatgatga gggaatatct tcttttggaa atagtgaact ttatgaccac
    10021 aaagatgcag aaggatttat taatttattc actttaccat taaaaatcag agctatgaaa
    10081 gctggaaaat aataaaataa attatttaag agtgtataaa aactattttt aggtttttat
    10141 atgctctttt tttatttgaa tttattaaat tatacagtaa atagtataaa aaaatatatt
    10201 atagttccaa atggcttaat aatgtgaaaa tataagacct aaaatataaa ttttataaat
    10261 acaatagtta ttgactctct cattgtaaga gggtttatac tgaatctaat gaaaatttat
    10321 agtgaggaga tagattatat gagaaaaata attgaaatta aacatcattc acatgattat
    10381 gagtgtatgt ggaatggtat agaagattta tatataaata aaaccggtga atgccttcca
    10441 aacgattttt tctttttact gtcaggtttt ggttcatttt gttatatgaa aacaaataaa
    10501 gctgatttga aaagaatggt agctttggga gatggaagga caaaacagat gtataaattt
    10561 ttagcaccaa ttgttggatt tgaatacaaa catcatgagt ttaaaaagta tgagcaagca
    10621 ttaaagaaag caaaatctga aattgatgat ggatatccag ttattttagg agctttagat
    10681 atgtattatc taccatatta taagaagcta tatcataaag aacatattcc ttttcattat
    10741 atattaatgg taggatatga tgatgaagga atatatctgt atgattgtgg tagaacagaa
    10801 ttattacatc tttcttatga taaattacgt gaaagcatga attgcaattc ccctggatta
    10861 agcaaagaaa atactatttg cacaataaga atgaatactt caaagaataa atataacatt
    10921 gctagagaag ctattgctat aaagcgtgat atgtttttaa atccacctaa aggatttttg
    10981 ggatataaag gttttgaaaa gtttattaaa gagttgccac agtggaacac tgaattggat
    11041 aaagatgatt atgataagat tttgaccaat atggttatgt tttttggaac agtaccaaca
    11101 attccaaatg caatcaaagg aattaaagaa ccagatactg ttcaatttaa aggtggattt
    11161 gataaaatga gtagaatgct caacagtatt ggggaggaat acaaaaacga ctcttttata
    11221 caagtagctg atatttttga taaaactgct attataattg agaaaatatc caatattata
    11281 atagattatt taactcaaac atgtgatgat acagaacaac ttctgttatt gttctctgag
    11341 gtcttagagc atatgaaaac aggatattta atcttggatg tataaaaaat atattgttga
    11401 ccctaacatt acgtaatagt gtaatatgaa aatatagctt gaatatatta gtaggtattg
    11461 atggctgtaa aattaaaatt tgaggtgtgg atatgagaac agtgaaacaa gtttctgatt
    11521 taactggaat aagtgtgagg gcattacatt attatgatga aataggtctt ttaaaaccaa
    11581 ataaaattac agatgcagga tatagacttt atgatgatga atctattaaa actcttcaac
    11641 aaattttatt ttttaaggag attgatattc ctttaagaga ggttaaggaa ataatgtcaa
    11701 gccaatattt tgataaggtt gaagctctaa aaaatcaaaa aaaattactt attttaaaac
    11761 gaaatagact ggatgaattg atagagctta taaatcaaac tctaagagga gaaggaaata
    11821 ttgattttaa agagtttgat atgagcgagt attttgatgt tttagaagaa tttaaaagag
    11881 agcataggaa taaggtaatt aaaatttatg gtagtgtaga aaaatacaat gaatatatag
    11941 aaagagttaa gtctaatgaa gagaaaattg ctaaaatggc tataaaacaa tatggaacta
    12001 ttgagaagtt tgcccaagct ataaaaacaa attttagcag tgatatattg aatttaggag
    12061 aaaaatttga tagatataaa aatgattgtt taaaagaaaa acatccaaaa ttaaaagaat
    12121 tatatagaaa gcttgtagaa gatttaagta ggaatccttc ttcaacagac cttcaagaaa
    12181 tagcaaaagc gataacagat atatctaaaa aagattatga aattttctct atggatactg
    12241 gtgatgacaa ctggtactat atggttcaaa attatctagt aaatcctacg tggatagaag
    12301 aagtagataa aaagtatgga agtggagcag gtaagtttat tggacaagta ctaaagactt
    12361 atctaagaga tagaaaacca aagataaata cattgtatga aaaacttgta gaagatttga
    12421 gtagagattg ttcttcaagg gaagttcaaa gcattgttga agaaatagac aatgaaatga
    12481 aaagaagtaa tgaattttat aaaatagaca atggtgaaag atactttgat tatatgtcag
    12541 agctttattt acaagattct aactatataa aagtaactga taagaattat ggtgatggtg
    12601 catctaagtt tattggagaa gcttttaaga tttattttga taataataat tgttgaaaat
    12661 ttggaagtaa aaaatatgta agttttataa aagcacaaaa ataaggatgt agattagctc
    12721 catctatatc cttatttaaa gttattaaaa tatatagttt acatcaatct taacactttc
    12781 aataatattt cccaaatatg tttttatact ttcaacttct tctaaagatt ttaatttatt
    12841 ttcagtataa attgctttgt ccagaatatt aaaccttaat cctatttcat caaagtactc
    12901 atgatttaaa actttgtacc aatggttttt ttctttattc caactcgtag atattgaatc
    12961 agcttctttt tgagcttttt cccaatcttc attttctata gatttttcta aaacagatat
    13021 attgtcttta tatccttctg taaaatcata tagtttattg ttaacgtaaa atccaaataa
    13081 tacaaataag atagtccata gtataacaaa tgttgtagat ttcaatatta atcacccttt
    13141 ttattttgaa taaatatttt gtcattttca tctaaaacac aaataagaac atctttaaaa
    13201 gtttctatgt gattagattt taaaataccc attaaccagt tagtatcttt ttgagctatg
    13261 tctagatttt tatttattat tctaccatcc agtataagtg ggataggtaa atgattaaaa
    13321 gctcttggag gagtactatt gtagcttgat gctggtacaa cacttagatt accatcagtc
    13381 tccaaaatag catattgtac atcttttagg ttgaagtatc cttgaatcct aagttgttct
    13441 aataattcat ctatactaat tctttctttt ttaagctctt tatatacaat ttttccttta
    13501 tctattaata cagaaggttt acctgataaa aatgaactta attttctgct ttttaatgat
    13561 aagaatgaaa taagagtctg catgattaca agaccagata ttgctgcaac accatttatg
    13621 attggtatat tattattttc cattgggaca gatgcaactt ctgcaattaa tagtgttata
    13681 actaaatcaa aacagttcat ttcagctatt tcaccttttc ccatgactct aagtgcaatt
    13741 agcactgtta tgtataatat aatacttctt attaatacta ctgtcatatc tatcaattac
    13801 tcctttaaaa aatatttaca cttagttagt atatcctata taaaaaattt aattcaaagt
    13861 ataaaaaacc ttaaataatt ggattcaact atttaaggtt tttttgataa ttttaatatt
    13921 aaatttttta tggattttct gttactttta tttattttaa taacagattc aacaaaactc
    13981 tttttatctc ctaattttaa gataaaatca ggatattctt caaattcatt gagttttatt
    14041 aatttgaaag aagaagatag atttttaa
//
LOCUS       DAEMHZ010000112        13962 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000112 DAEMHZ010000000
VERSION     DAEMHZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.112"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            157..297
                     /locus_tag="KPK72_002894"
     CDS             157..297
                     /locus_tag="KPK72_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490092.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(472..792)
                     /locus_tag="KPK72_002895"
     CDS             complement(472..792)
                     /locus_tag="KPK72_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2490093.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(912..1649)
                     /locus_tag="KPK72_002896"
     CDS             complement(912..1649)
                     /locus_tag="KPK72_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG2490094.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1864..3687
                     /gene="acd"
                     /locus_tag="KPK72_002897"
     CDS             1864..3687
                     /gene="acd"
                     /locus_tag="KPK72_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG2490095.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3902..8200
                     /locus_tag="KPK72_002898"
     CDS             3902..8200
                     /locus_tag="KPK72_002898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG2490096.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8404..8865
                     /locus_tag="KPK72_002899"
     CDS             8404..8865
                     /locus_tag="KPK72_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG2490097.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8895..10049
                     /gene="nusA"
                     /locus_tag="KPK72_002900"
     CDS             8895..10049
                     /gene="nusA"
                     /locus_tag="KPK72_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG2490098.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10056..10337
                     /locus_tag="KPK72_002901"
     CDS             10056..10337
                     /locus_tag="KPK72_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG2490099.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10327..10638
                     /locus_tag="KPK72_002902"
     CDS             10327..10638
                     /locus_tag="KPK72_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG2490100.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10660..12600
                     /gene="infB"
                     /locus_tag="KPK72_002903"
     CDS             10660..12600
                     /gene="infB"
                     /locus_tag="KPK72_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG2490101.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12643..13023
                     /gene="rbfA"
                     /locus_tag="KPK72_002904"
     CDS             12643..13023
                     /gene="rbfA"
                     /locus_tag="KPK72_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG2490102.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13004..13681
                     /locus_tag="KPK72_002905"
                     /pseudo
     CDS             13004..13681
                     /locus_tag="KPK72_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5578 a         1597 c         2622 g         4165 t
ORIGIN      
        1 aataggtaaa atatcctatt gttttgtaga tatatcctaa ttatgtcaaa aaataaaaaa
       61 atttttattt attagagttt ttggaaataa taataacaga tattataatt aattaacagt
      121 ttttgtaagt atttgacaat agagagggtg taacaaatgg gaataataga gtcagcatca
      181 aaattggctg agatggttca tttattggct gttgaaaagg ggataactga tatagaagca
      241 tgggatgaag ctgtgaaaga atacagcaaa atttatgaag aaagaagaaa tgaatagaat
      301 gataaaaaat gaaaacttga tgataaaaaa taactttatt taaagaaatt caatttttat
      361 agaagtatta aaaataatat aaataaaaag atggtatttc aaatgtaagt aaaaaatcat
      421 tttgggatac catccttatt atttatttta ataatatata agtaataaat cttacttatc
      481 catatttttt atgtaacctc ttttttctaa cttttttata tgctccttac gtatttcatc
      541 aagattaata ccatattgtt cttctagctt aaacatcata gtaacacatg tctgcgctac
      601 atccattagt tccttagcag ttttctttat aatatcaact tcatctaaat cattattttc
      661 tccacttaga tttctgaatt tacctataca ttcagctaat tcaccttgtt cttcacccat
      721 tttgataaat gtagattcta gtgttgggtc taaattatta agctctggta aagatatagt
      781 ttttagtttc ataaaagctc ctttcaagtt attcgtattt atactaaaac atcataacaa
      841 aaataatact aaaaaaaaac tttaaattca acataaaaac ttacatatat aataaaaatc
      901 gtgaaataaa tctatatttg tcttaataca gcctcatata aaacttctcc aataggttta
      961 tttattacaa gagaagctga cttatcatag actgtatttg ctttattaat aagagcaata
     1021 taatcacctt tgtaataatc tataaaactt gctgctgggt acacagctaa tgatgtacct
     1081 cctataatca ataaatcagc attacttata gcagaaattg ttttagtaat gacatcactg
     1141 tcaagagctt cctcataaag tactacatct ggttttacta tagaaccaca gttgtcacag
     1201 taaggaatat tacctcccag atttaacata gattctaagt caaaaaactt accacattta
     1261 gtacaataat ttctatgtac agaaccatga agttctagca catttttact tccagccatt
     1321 tgatgaagtc catcaatatt ttgagttatg acagctttaa gcttacccat ttcttcgagt
     1381 tttgctaaag ctatatgagc attattgggt tttgcattag gatatattaa tttatcctta
     1441 taaaattcaa aaaattcttc tggatatcta acaaaaaaag tatgtgaaac taattgttca
     1501 gcagtaaatt gtttattgag tttttggcta aataatccag tagaacttct aaagtctgga
     1561 atattagatt cagttgaaac acctgctcca ccaaaaaata caatattatt gtgattagct
     1621 attaagtctt ttaagctatt tgcatccata gttacacctc caaattatta tatataaatt
     1681 gtactacata ttaggtcgtt tatgttttat taatttaaat aaatatatag tttaataagt
     1741 taaataaagg gaatataaat aaaatgtata cagagtataa atagacaata tttactaaaa
     1801 taacaagttg tcgtataatt atattaaaat tgttttagtg tataagaagg gcgggggaag
     1861 tacgtgaaga aaaaagcagc tttagcaaca ttggctatgt tacccttagg tgtggtaaat
     1921 gcacatgctg atggagatat aggtatagtg actataaatt atttaaatgt aagaaatgaa
     1981 ccaactgccg aaagtagcat agcctttgtt gctaaaaaag atgataaagt tttgattaaa
     2041 gactcttcta atggatggta taaaataaaa gctgaatctg gacaagaagg ttgggcttca
     2101 tcaaaatata tagcaaaatc aaatagtgac tctttaagaa cgtctacaaa taaagaaaaa
     2161 caagttattt caaatagttt aaatatgaga aatggagcag gtacaagtta cagagtaata
     2221 actgtactaa aaaaaggtca aaaagtagaa gtaatatcag agagtaatgg atggtctaaa
     2281 atcaaatatg atggaagact aggatatgta tctagctctt atttaggaga cgtttcaaac
     2341 tcaactaata aatcaaaaac taaacaagtg aatacaactt cactaaatgt aaggagtgga
     2401 ccaaatacaa gttatggttt gttaggcaag ttgccaaaag gaagtaaagt agaagtaata
     2461 tcagaaagta atggatggtc caaaataaag tataatggaa aagatgccta tgtatctagt
     2521 atgtacttat cggatgtaag tcaaagtaat tcagacaatt ctagtcaaag caatgataaa
     2581 aagaatactg ataaggttgt aaatacagct tctttaaatg taagaagtgg accaggttct
     2641 acatatagta agttgggaaa agtttataaa ggaagtaaag taactgtact atcagaaagt
     2701 agtggatggg ctaagattaa ttttaacaat aaagaagcat ttgtagtagg caattattta
     2761 tctacctcag cagatacttc aaataataac tcaaatagta actctgataa cagttctaat
     2821 agtaatggta ataattcttc atcatcaggc caagtaaatg gtatgtcagg tataagtggg
     2881 gctaaaattg attataaatc tttgagttat actttagaat ctcatataag taaacaagtt
     2941 gagaaagcag catcaggagg aaatgtgata gctccaagta ataggaaaag tactccaagt
     3001 cctgagttta gtactttttc agctcaaaga acaagctcat ttgtaaatgc aagttcaagt
     3061 gatatagaat attatttaaa tcctaagaat tttacaaata ctactaaagg tatgatgcag
     3121 tttttaaaga ttaacagtta tagagatggt atttcagaat caagtcttaa ctcgtatctt
     3181 aatggtttat cttctagtgt atttaaaaac cagggagcag cctttataaa tgctgccaag
     3241 aagtataata tagatgttgt atatctagta tctcatgcta tgtgggaaac tgcttatggt
     3301 aagtctacac ttgcccaagg tcaaacatta acatcttata agggacaacc tcttagtaag
     3361 ccagttaaag tatacaattt ttttggtata ggagcaatag ataaaagtgc taatgtttca
     3421 ggtgcagaag cagcgtattc caatggctgg actagtgtag aagctacaat agatggctct
     3481 gcaaagtgga tatcacaaaa ttacgtaaat agttctaagt acaatcagaa tactatctac
     3541 aagatgaagt ggaattatga ttatacatgg catcagtatg caactgatgt aaactgggct
     3601 aatggaattt ctggaattat ggaaaattta attggtcttt atggaggagg aagtagtttg
     3661 gtttttgaag ttcctcaata taagtaatac ataaataaaa aatatagact atgggtagtt
     3721 ttcattttat ttaaattatc tatagtcttt ttgtatataa tttataatct gtaaatttaa
     3781 tgttaacaaa acttttatgt tataatatta attctaagta aatataagtg agtaataggt
     3841 agaaatagat aaaaagtggt aataatatat ataaagataa ttttgagaaa ggaggatata
     3901 tatggaaagt attaaggaat atttggataa gttagaaatt aataatagtg gtcttggaaa
     3961 gcaacttaaa gaagtttata taaatagagt agtttatttt aaggaagata agatagttta
     4021 tttttatcta acttctaaag atattgtttc tcatgaactt ttagataaat ttaaagaaga
     4081 attgatgtat aaactcgatt attttaaaga tatgaggatg aagataagat ttactggtct
     4141 tgaaagaaaa tccaataaag atgttataaa aaaatattgg aataatatat tgtatatatt
     4201 aaagtatctt tgtccatcta ttgcagggtg gtataaacaa gtagaatttc tttgtttaga
     4261 agaagaatta aaaataaaac ttccaaaagg tatattttat gagcgattaa tgaaaaaaaa
     4321 tgtaacttat gtacttaaaa ccgtgcttag cgaagagttg ggattagatt taaatataac
     4381 aatagaaaaa gctgttgatg aaaaggtaaa taaagaaaga ctcataagaa tcaatgatag
     4441 agaaatggaa gaaaaaatta gggctttaga aattggaaaa gttaataact gtgaagaaaa
     4501 tgaagaggaa agctatgtca ttaagtctga tgtagatgaa aatttaatct atggagataa
     4561 tgcaaatgct atgattgaaa atatagttga attaaatgca tcttctggaa cagttgcagt
     4621 tgttggagat atatttgatg ttgacactaa agaacttaaa aacggaaaaa tacttatgat
     4681 agcttcaatt acagattata ctagctcaat aagctgtaaa ctttttttaa cagatacaaa
     4741 taaggatgga gtacttgaaa atgttaaaaa aggagcatac ttaaaaatca agggtgacat
     4801 agtttatgat acatatcaaa gagaaattag tatgatgatt agtgggataa gaaaagaaac
     4861 tagaatagag agagaagata aatcagagga aaaaagagta gaactccatg cacatacaca
     4921 aatgtcttct atggatgcta tttgttctgt taaaaagctt gtagagcgtg cagccaaatg
     4981 gggtcattct gctattgcta taacagacca tggtgttgta caaggatttc cagatgctat
     5041 gaatgcaggt aagtcaaata atataaaaat actttatgga gtagaagggt acctggttga
     5101 agatgattct ctcattatag aggatgcaaa tgataaagaa ctttcgcaga catttgttgt
     5161 atttgacata gagacaactg gcttttctaa tacaaatgat aaaataactg agattggtgc
     5221 tgtaaaaata gaaaacttta agattgtaga tagatttagt gagttaataa atcctgaaaa
     5281 agatatttct tataagatac aggaattgac aggaataact aatgaattaa ttaaagacaa
     5341 gccaaccatt gaagaagttc ttcctaagtt tatggaattt gttggagata gtgtattagt
     5401 agcacataat gcagagtttg atactggatt tatatcgcaa aaatgtaggg aacaaggctt
     5461 gttatataat aacaaaaaag tagatacact tatgttagca agagtaatgt tgccaaactt
     5521 aaaaagacat agattaaatg tcgttgcaaa agaacttggt attcctcttt taaatcacca
     5581 tagagcagtg gatgatgctg aagctacagc acttatattt aataagtttt tgcagatgct
     5641 tacagaaaaa ggtgcaaaaa ctctaagtga tgtaaacaat atacttggaa agattgatta
     5701 cacaaagctt agcacaaatc atataacctt gatagcaaaa aattctatag gtataaaaaa
     5761 tttatataaa ataatttcag atgcacatgt aaatcatttt tttagagcac caagaatcct
     5821 tagaagtgta ttaaatgaat ataaagaagg attaataatt ggttcggctt gtgaagctgg
     5881 ggtggttttt caagctgtaa agaaaaacgt gtctgatgag gaaatgaaaa aaataataga
     5941 tttatacgat tatattgaag taatgcctat agataataat agatttatga ttgataaagg
     6001 agaagtaaag gacgaagaag agttaaggga gttgaatagg aaactaattg atacagccaa
     6061 aaaatttgac aaaattccag tagctactgg ggatgttcat tttttagaca aacatgaagc
     6121 tgtcttaaga aaagttctta aatattctca aggttttaaa gtagatgaag aagaaactta
     6181 tttacatttt agaacaacag atgaaatgtt ggaagagttt agttatcttg gagaagaatt
     6241 ggcatatgaa gttgtagttg aaaatagtaa tctgattgcc gatatggttg aagatataaa
     6301 acctatacca gatgatactt atcctccaat tatagagggc tcagatgtgg agttaagaga
     6361 aatgtgttac aaaaaagcca aacgaatcta tggagaccca ataccagaaa tagttcaaaa
     6421 aagactggat agagaattaa attcaataat aagcaatggg tatgcagtta tgtatattat
     6481 agctcaaaaa ctggttgcaa aatctctttc agatggatat ttagttggtt caagaggctc
     6541 agttggctca tcttttgcag ctactatgag tgatataaca gaagttaatc ctcttccagc
     6601 acattatatt tgtgaaaacg aagactgtaa atattcattc ttttatgaaa taggtgaatg
     6661 gggctcaggt gttgatttac cagacaagga ttgtccaaag tgtggaagaa aacttgctaa
     6721 aaacggtcat gatattccat ttgaggtttt cttaggtttt gaaggtgata aggaaccaga
     6781 tatagactta aatttctcag gaaattatca gccaacaatt cataaataca cagaagaact
     6841 atttggagag gggtatgtat atagggctgg gactataggt actgtagcag aaaaaacagc
     6901 ttttggatac gcaagaaaat atgtggaaga aaataatata agtgtaccaa atgcagaagt
     6961 acttaggctt tcaaatggat gtactggagt aaaaagaacc tcaggacaac atcctggtgg
     7021 agttatggtt ataccagact ataaagatgt atacgatttt acacctatac agtatccagc
     7081 aaatgatact tcttgtggtg ttataactac tcactttgat tatcattcaa taagtggtag
     7141 aatacttaaa cttgatatac ttggacacga cggtcctacc attataagga tgcttgaaga
     7201 tatgactgga ataaatataa cagaaattcc tttagatgat aaagagacta tgtctctatt
     7261 tacatctaca gaagcattag gtgttacacc agaagaaata aactgtccta taggttgcct
     7321 tgctgtacca gagtttggaa ctaagtttgt acgacaaatg ttacttgaca caaaacctac
     7381 tacatttgca gaacttgtac gtatatctgg tctctctcat gggacagatg tttgggtaaa
     7441 taatgcacaa gacttagtta gagaagatat agttggatta aaagatgtta tatctacacg
     7501 agatgacata atgaactatt taatatttaa aggattacct cccaaaatga gctttacaat
     7561 aatggagagt gtaagaaaag gtaaaggttt aaaaccagaa cacattgaag aaatgaaaaa
     7621 aaataatgtt ccagaatggt atataggctc atgtaaaaag attaagtaca tgttcccaaa
     7681 agcacatgct gttgcttatg taatgacatc atttagaatt gcatattgta aagttcacta
     7741 tccagaagca ttttatgcga cttattttac gacaaaagtt gaggattttg atgcagattt
     7801 aattgttaaa ggattagatg ctataaaatc aaaaataaat gaaattgagt cactgggaaa
     7861 tgatgcaacc acaaaagaaa aaggtatgtt gaccgtttta gaagttgctc ttgaaatgta
     7921 tgctagagga ataaaattat taccagttga tatatataaa tcagatgcaa ctgagtttat
     7981 agttgtagga gaaaaaacat tacttccacc aatggcagca atacaaggtc ttggaggaaa
     8041 tgcggctata aacatccaaa atgaaagaaa aaatggtgaa tttatatcaa aagaagattt
     8101 gagaaagaga acaaaaatat caaaaacggt aatagaaact cttactaatc atggttcact
     8161 tgagaatatg agtgagagaa atcaattgtc attgttttaa gttagcccaa aaagttgcaa
     8221 ataaaagaat tttatgatat catagtatag aaaatagtat actttgcaga aactaagagt
     8281 gagacagctg ttctcactct tttattttaa atatattatt aaaaagctgg tttattttta
     8341 ttaaatatat tttatttaag attatataaa ctggtcattt acaattgaat aggaggaact
     8401 ttaatgaaaa aaaatttaga agctacaatt gaggagattg taactaaaat aacggatgaa
     8461 catggatttg aaatggttga tgttgaatat gtaaaagaag caggagaata ctacttaaga
     8521 gtatacatag ataaagaaga aggaatatct ttaaatgaat gtgaattagt tagtagagaa
     8581 ttaagtccaa tactagatga aaaagaccct ataaaagaaa attatttttt agaggtatct
     8641 tctccagggc ttgatagagc attaaaaaaa gacagagatt ttgttagata tcaaggtaga
     8701 gatgtagatt taaaattata taaaccatta aatggatgta agcaatttga aggtgaactt
     8761 gtaggattaa cagaagataa taatataaaa atcattgcaa atggtaagga aatggaattt
     8821 aatagaaaag atgttgctat tgtcagatta gcaataaagt tttagtggta tattaaatag
     8881 ggaggatata aaaaatgaat catgaattta tggaagcatt agatgaatta gtagttgaca
     8941 gaggtataga taaagaaata cttatagata ctatagaaca agctctttta actgcataca
     9001 agaaaaactt tggttcagca caaaatgtta gagttgaatt tgatagagac aagggagata
     9061 taaaagtatt ttctcaaaga gttgttgttg atgaatcaga tttatatgat actttcttag
     9121 aaatagagtt agcagatgct agagagataa atcctaatta tgaattaggt gatattatag
     9181 aacatgaagt tacacctaaa aactttggaa gaattgcagc tcaaacagca aagcaagtag
     9241 ttgttcaaag aataagggaa gctgagagag aaatagtgta taatgaattt atagaaaaag
     9301 aaaatgaaat agttacagga gaggttgcta gagcaaataa aaatgttgtt tatgtaaatc
     9361 ttggaagaat agaaggtata atgactcaaa gtgaacaaat accatttgag aattataaag
     9421 caggtcaaaa aataaaattc tatatacttg aagttaaaaa gactaacaaa ggacctcaaa
     9481 tagttgtatc aagaagtcat ccgggtctag taaagagatt gtttgaagaa gaagttcctg
     9541 aaatatatga aggaatagtt caaataaaat ctgtagcaag agaagcaggg tcaagaacta
     9601 aaatggcagt aagatctata gatgaaaaaa tagacccaat tggagcttgt gttggaccaa
     9661 aaggacttag agtaaaaaat atagtggatg aacttggcga tgagaaaata gatataataa
     9721 aatatagtga agacccagca gagtttatat cagcagcact tagcccatca aaggttgtta
     9781 gagtagatat agatgaagat gaaaagtctg ccttagtaat cgttccagat tatcagttat
     9841 cgcttgctat aggtaaagaa ggtcaaaatg caagactagc agcaaaattg actaactgga
     9901 aaatagatat taagagcgaa tctcaagcag aacaagaaat gttaaatctt gaaaattcca
     9961 aagaaataga agctgaggat gttgaagctg aatttatcga ccaagaagag ttagatactt
    10021 cagaggataa ttttagtgaa gaagagtaag gagggatgat tttgaaaaaa gtaaaaaaag
    10081 tgcctcaaag aaaatgcata gcatgtcaag atagagactc taaaaaagag cttataagaa
    10141 tagttaaaaa taaagaagga aaaatatttt tagacccaac aggaagagcc aatggaagag
    10201 gagcatatgt atgtaaaagc tctgaatgtc taaaaaaagc tataaagagt aaagcattaa
    10261 ataaagcttt taaaatagat gttcctgatg aagtctatga taatctactt atggagttag
    10321 aagaatatga aaaataataa agaaaaagta ctttcgttct tagggctcgc aactcgtgca
    10381 gggaaaatag tgtctggtga tgattcaact ttgcttgacc ttaaaaaagg aaaagtaaat
    10441 ttgattttaa tcgcagaaga tgcatcaaat aatactaaaa aactttttaa agataaatca
    10501 acttttagaa atattccata tctatttttt tcaacaaaag aggaaatagg atttgcaata
    10561 ggaaaatctc caagagcagt tgtaggaata aaagatgaaa atttttctaa aaagataatt
    10621 gaactaatag aaatttaaaa taaaataggg ggtgatcttg tgtcaaaaac aagagtatac
    10681 caaatagcag aagaattaaa tatatcaaat gaagagctta taaataagtt agctgaatta
    10741 gatataaatg taactgacaa agattcagta ttagaaggtg aagagttaga attagcactt
    10801 gaaatgttag gtgaagattt atctcaagaa aatggaaatg ttatagaaat agatggaaag
    10861 ttgacagtac aagtattagc aacgaaatta gataaatctc catcagaaat aattatgaag
    10921 cttatgaaaa tgggaactat ggcaacaata aatcaagaga taagttttga gattgctgct
    10981 ttggctgcca aagactatgg atttgaatta acagtagctg aaagtgatga tacagaagct
    11041 ttagaaatag aagctttaat ggaaattgaa gaagacaaag aagaggattt aaaacctaga
    11101 ccaccagttg ttacagttat gggacatgtt gaccatggta agacatcttt actagatgca
    11161 ataagaaaaa ctgatgtgat ctcaggtgaa gctggaggaa taactcagca tataggtgct
    11221 tcagaagtta aaatcaatgg acacaaaata gtatttttag atactccagg acatgaggct
    11281 ttcacatcaa tgagagcaag aggagcacaa gttacagata tagcaatatt agtagttgca
    11341 gcagatgatg gtataatgcc tcaaacagtt gaagctataa accatgctaa agcagcaggt
    11401 gtacctttaa tagttgctat taataaaata gataaaccag gtgcaaaccc agataaggtt
    11461 aaacaggaat tagcagacca aggattatta gttgaagact ggggtggaga agtaatagca
    11521 gttccagtat cagctaagaa aaaagaagga atagatacac ttttagaaat ggtattattg
    11581 gttgctgaaa tggaagagtt aagagctaat ccaaataaaa gagcagtagg aactgttata
    11641 gaagctgagc ttgataaagg aagagggcca gttgcaacag tacttgttca aggtggtact
    11701 ttaacagtag gtgacccaat cgttgcaggt gttgcatgtg gtaaagttag agcaatgata
    11761 aatgcaaaag gtaagagagt aaaaacagca ggaccatcta cagcagtcga gatattaggt
    11821 ctatcagaag ttccacaagg aggagaccaa tttgtcgaag ttccaactga taagattgct
    11881 agaagtgtag cagcaagaag acaacaaata gttagagatg aaatgttaaa atcaactcaa
    11941 agattatcac tagatgctct atttagtcaa atgagtgaag gttctataaa agaccttaat
    12001 atagttataa aagcagatgt acaaggttca gtacaggcag ttaaacaatc tttagaaaaa
    12061 ttatcaaatg aagaagttca agttaaagta atacatggtg gtgttggagc tgtaacagaa
    12121 tcagacatat tacttgcagc agcttctaac gcaataataa taggattcaa tgtaagacca
    12181 gttccaggtg cagaatctct aggagaaaaa gaaaatgttg atataagaac atatactata
    12241 atatacaagg caatagaaga tatacaagcg gctatgactg gtatgttaga ccctgaatat
    12301 gttgatgaag aaacaggaaa agctgaaata agagaaatat ataaaatatc tggtgttgga
    12361 acagtagctg gatgttatgt gactaatggt aagatattta gaaattgtaa agtaagatta
    12421 gttagagata gcataataat acatgaaggt gagcttgcag cacttaagag atttaaagat
    12481 gatgtaaaag aagttaattc tggatacgaa tgtggtatga gttttgtaaa ttataacgac
    12541 ataaaagaag gcgatatagt agaagcttat ataactaaag aagtagaaag aaaattataa
    12601 attcgtatca aagaaaatca gttagtaaag aaggtcgatg gtatggcatc atataataga
    12661 acaagaagga tagcagaaga aataagaaaa gtcgtaagta ctatgcttat aaatggagta
    12721 aaagacccta gaataacatc tatggtgagt gtaactgatg tagaagttac aaatgaccta
    12781 agatatgctt atgtatatgt aagtattcta ggcggagatg aagaatccac actaacaggt
    12841 cttaaaagtg ctggaggata tataagaaga gaagtaggaa aaaatataaa acttagatat
    12901 attcctgaga tagtatttaa acttgatgat tcaatagaaa aaggaatgta tatggatagc
    12961 cttataaaaa gagttaatga aaaaaatgct caacaaaatg aggatgaaaa ttatgatgaa
    13021 taatatggat agtattataa actatattta tgagggtgac tattttatag ttacctctca
    13081 tatcagtcca gatggagaca atattggttc tacactttca atgtattata cattgaagaa
    13141 tttaggaaaa aatgtatatt atgtacttga tgatgaagct ccattaaatt taaggttttt
    13201 ggttgaaggt gtaaaaatct ataagtctaa tgaatttaat atgaagaact attctctaat
    13261 tgcacttgat tgtggggata agatgagaat atgtgtgagt gatgaaatca aaaataatgc
    13321 atcaaaatta atctgtattg accatcatgc tagtaatgat tcatatggag atttaaatta
    13381 tgtagatatt gatgcatctt ctacatgtga attagtttat aatttattag ttagatttca
    13441 gcaaataaaa gatattaata ttataaatga agatatagca acatgtttat atacaggtct
    13501 tgtaactgat actggaaact tttcgtattc aaatgtacat gcaagtagct ttgaaatggc
    13561 aaaaaattta cttgttttag gtgcacaaaa aaatactata atacaaaata tatatcaaag
    13621 caattcttct ggttattaca aattattagg agaagctcta aagggtttgg agatatttga
    13681 ttaaaaaata gttacgcaca ctcgtgactt tagtcatgag ttagtagcta aaatagtcag
    13741 catataggga aacttatatg tagagatagg ataaaaccta tccaatacta cttgaattgc
    13801 tggaaacccc taaagctaac caaactaaaa cataaggatg aaataaacct aagtgtgaaa
    13861 gttacgaaag tagaaaaaat tggttagatg gtataaggtt aaaccctaag tactttaaaa
    13921 tgggcaatca gcaggtaagc tccgaatagg agaaacttca ac
//
LOCUS       DAEMHZ010000113        13616 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000113 DAEMHZ010000000
VERSION     DAEMHZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.113"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            250..2484
                     /locus_tag="KPK72_002906"
     CDS             250..2484
                     /locus_tag="KPK72_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2490103.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMFQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2515..3360
                     /locus_tag="KPK72_002907"
     CDS             2515..3360
                     /locus_tag="KPK72_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2490104.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3600..5333
                     /locus_tag="KPK72_002908"
     CDS             3600..5333
                     /locus_tag="KPK72_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2490105.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5725..6216
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5794..5822
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6496..6648)
                     /locus_tag="KPK72_002909"
     CDS             complement(6496..6648)
                     /locus_tag="KPK72_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490106.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            7772..9127
                     /locus_tag="KPK72_002910"
     CDS             7772..9127
                     /locus_tag="KPK72_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2490107.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9245..9913)
                     /locus_tag="KPK72_002911"
     CDS             complement(9245..9913)
                     /locus_tag="KPK72_002911"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG2490108.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(9980..10408)
                     /locus_tag="KPK72_002912"
     CDS             complement(9980..10408)
                     /locus_tag="KPK72_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2490109.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            10515..11108
                     /locus_tag="KPK72_002913"
     CDS             10515..11108
                     /locus_tag="KPK72_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG2490110.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11228..12163
                     /locus_tag="KPK72_002914"
     CDS             11228..12163
                     /locus_tag="KPK72_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG2490111.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(12434..13378)
                     /locus_tag="KPK72_002915"
     CDS             complement(12434..13378)
                     /locus_tag="KPK72_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2490112.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         5105 a         1841 c         1782 g         4888 t
ORIGIN      
        1 gatataattg cattcaaaaa ggaaatcttt caatatacta gaatttaagc attaactaat
       61 tttatctaat attattacta aactaaaaaa tatttgtatt tgtagcattg gagcaaataa
      121 cttaatgtat attcatttta aatacttatt taaaatttag catttaaatt atttacaaat
      181 taacatttaa atacttctag cataaataca gttgtcatca aaatttaaag tatcttatgg
      241 aggaatacca tgcaaaatca taattatata aaaatgttaa ataaaaaggt agaagtatta
      301 tctaaggaat tagatagtag aaattttaaa ttaaaaacct taaatgaaat agattttttt
      361 tctaaaatac caaacagaga atttatgtac caacatattg atgactgtat ttcgaaaaat
      421 atacatatag gaatcatgtt attaaatatc gataacttta aattatttaa tgatatatat
      481 ggatacctag tcggagaaaa tttattaatt actctaactc aaaacctatt gcactttatt
      541 ccagaatcaa agggaatagt cgcttattta aaaggagaag gttttttaat tgtacttcca
      601 aatgtatcaa ataaagagtt aaataatatg ggtgcaacta ttatagagag cgcccgagaa
      661 ttaaaaataa aacttcctaa tgaaaaagaa atatactcca atgttacatt tagtatcggc
      721 agcactatct ggaatggtga accaaacatg actactttga tgcttttcaa ccaagtatat
      781 agtgctttaa aaaaggctaa aagtgaaggt aaaaatagat acattctatt tgattttaaa
      841 gataatagtt tttacaataa attagaaaat gataaggagt tcttatcaga aatatcacat
      901 gttctacaaa ctaaacaaat tgaagtattt taccaaccac tttataatat aaaaaataat
      961 actatagttg gatgtgaggc acttgcaaga tggagacacc ctattaaagg acttcttact
     1021 ccagataaat ttattccttt atttgaacag tttggtctga taacggaatt agatttgcat
     1081 ttatttgaag agaactgtaa aaatataaga agatggattg ataacaatga tgtttttgtt
     1141 ccaatttgtt gtaacttttc tcgtcttcat ttttttaacc ctaattttcc aagtatgcta
     1201 aaaggtatta ctgaaaagta cagcgtctct acatcaaatt ttagcattga aataacagaa
     1261 aatatattaa tagaaaatac aaatacaatt attcaacaac tagctgaact tcgttcttat
     1321 ggtttttctg tatcaatgga cgattttggt tctggatatt catcttttgg tatgtttcaa
     1381 aatctacctt ttgatataat caaaattgat aggatatttt tatctcataa tttaaatgat
     1441 tttaaaaata caaccatttt gtactctata ataaacattg caagagctct tgggatgctt
     1501 acagtatgtg aaggtgttga aacagaacaa catgtagaat ttattaaaag tataaattgc
     1561 gatatcgccc aaggttatta ttatgcaaaa cccatggagt gcaaagaatt tgaaaaatta
     1621 ctatctaaag agaataattc agagaaaatg tttataaaat attctgatat aggaaaatct
     1681 ttaatagaga agatatttga tggatttttt attatgcaag atatgaaaaa actaagagct
     1741 aatgtcactg aagacattca atggtttgat ttttacaaca aaactgaaat tacatctttt
     1801 cctaaagtaa tagaacattt tgaaaaacat attaaaggta aaaaattttc tattctttac
     1861 aaaagtataa ccccacataa tactgaatca gattcattat taattagtgg agaagccgtt
     1921 ttatcagatg aagcttctga acatcaaaaa tatagcaatt tctactttag tgcaaattgt
     1981 ataatttcag aaaataaatt actcttagca aaattacatt tggatttaat taaaactgct
     2041 ggctatataa atgaatttga atcatattca atacctaaaa tgacaaatac acatcttgat
     2101 gaaagtaata ttttatcaca attttattca atgttgcctg taggtataat tcgctacgat
     2161 ttatttggtg atatgattat tacttatatg aatgaagaaa tgtttgatat tataggttat
     2221 acaaaagaac agttttttgg tgagatgggc ggaaatttaa gagcaattgt tcatccagat
     2281 gatttagact ttgtttataa aaaaagtttg gacatgattg acacaaataa aattgaacca
     2341 tttttatata gattcataaa acgagatggt caaattgtca cagttatgta tacacaatgt
     2401 aacctttctg caattgatgg aaggtcgatt gcccagggta tgtatgtaga tgtaaataaa
     2461 ataaaaaatt taacaaaaat ctagtacaaa gtattttatt ttggagaaat taatatgttt
     2521 aaagaaattt ttttaagaac ttttccagga tttctaatta taagagattc taactacaga
     2581 attatattta taaatgataa tttaaaaaat ttaattaaaa gttatatgca agataatcca
     2641 cttggaatga ctaatataga aatagctaag aaactccctg ataatattgc taaatttttt
     2701 acggattctc acaatattca attagattgg gaaaaaaatt atccttatga taaaatttca
     2761 aattggattt tagagtttaa aaaagatact acaagctatt ggaatgtttt agaatataaa
     2821 gtagatgttg atgaaaaaac ctacataatt actatggcca atgatataac aaaactctat
     2881 gaagaaaata agagaaatct tcactactca attactgacc cattaactgg agcatataat
     2941 agaaaatatt taaatgatag atttgacata tttataggag attatattgt actaattgac
     3001 ttagataatt tcaaaatgat aaatgactat gaaggtcata atgtaggaga caagatttta
     3061 tgtgattttg tttcactttt aaataaagaa ttaataaatt ctacttctat tataagatta
     3121 ggaggagatg aatttattgt tatattctct agtgatgttg acaaaaatta tgtttattca
     3181 cagctagaag ccttaagaga aaatttttta aaagtattct ctaagtataa gtatctttca
     3241 tttagttatg gtgttgatac agtaaaaaga aatttaaaat tgacaatagt tgaattggat
     3301 aaaaaaatgt ataaaaacaa ggagaaaaac aagaaaaaat ttgataaaaa tgattattaa
     3361 cattgttatt ttaaaataaa ttaaaattat ctttcttttt ttaaacttgt acattagttt
     3421 ataaatatgt atctatacaa gatattgaca ttaagtactg aagcatatat tgtttcagtt
     3481 cttttttgtt aaaattaact tatttaacaa agatatcata gtttaacaaa gcttttaatt
     3541 taaatatatt gatgtataat ataattaaaa tataatagca aaaagaaagg aggaatatca
     3601 tgagcatact cctaaaaaaa gcacccaaat tagcaaaaca tattataaca agtttttata
     3661 taaatcgaga tatcaatgaa gtattaaaat acctttgtga aaatgttaca tggattggac
     3721 caggagaaca ggaatttctc acttctttta atgaaataaa aaattatttt tatgcaggtc
     3781 aagatgaaat tccttcttgt gatataaata atgatatatt tgaaactgta agtgaagatg
     3841 agaatagatg tatggtatta ggaagatata ctgttcgaac taaagaaaat gctcaaatga
     3901 ttttagaggt aaatcaacgt tgtacatttg agataattga ggatagagga aaattattaa
     3961 taaagcacat gcacatatca aacccttatg gtgaaatgca acttgatgaa tactttccca
     4021 ctaaaattgg aactcaaagt tatgattatt tgcaaagatt actaaaagaa aaaacagaag
     4081 ttattgaaat gattacaaat aatatcaatg gtggcttaaa aggtagcaat gatgatagta
     4141 catactcatt tttctatgtc aatgagggat tgccaaaaat attaggatac acatatgatg
     4201 agttcatgga aatgagtggt ggttcagcag tgggtgctgt atatccacca gacttaccta
     4261 aagcactaga agattgtgaa cgatgttttg caaaaggacc tacatattca tctgaatata
     4321 gaatccgaaa aaaagatgga acattaatgt gggtattaga ttctggaatg aaatcgttaa
     4381 atagtgatgg catagtaaaa attaatagta ttattactga cataacccaa ctaaaaaaca
     4441 tagaatctga actaaaatta gaaagagagc gatatcgtat tgctttgcaa aatattacag
     4501 atatcatgtt tgagtatgat attgaaaacg atacccttat aaaatatcaa cgtgtagaaa
     4561 ttgacaaaaa aattgaatta gaaaattttg aaacaaaaaa ttactccaaa ctattagaat
     4621 ctggtaaaat tattcacctt gatgatattg gaaaactact agaagtactt catggaaatc
     4681 ttcgtgaaac aattgagata agacaagtaa attcattaac taaaaatgaa tggagatgga
     4741 ttcgtgttca atgttcagtt atatatgata gtgaccataa ccctatcaaa acaataggcg
     4801 ttttaaaaga cataactgaa gataaatcta aattagaaag tttaattaat caggctcaac
     4861 gtgacccact aacacaatta tataatcaaa gagtatcaca aaatcttatt gaagagtatc
     4921 tatgtagttc tgattccaaa aataatgatg ctctattagt tattgatatt gatgatttta
     4981 aaactgtaaa tgatactttt gggcatctag agggaaatga agttttagtt gctgtatcaa
     5041 aaatactatt acataacaca catgataaag acatagttgc tcgaattggt ggagatgaat
     5101 ttacaatatt tataaaatca ttaacaaagg atttaataat aaaaattact aatgatattt
     5161 taaatgatgc gagtaaaata aaagtaaagg acaatcataa aataacatta agtattggta
     5221 ttgccttcac tgatgattca actaaattat ataaagattt attttctaaa gctgataaag
     5281 ctctttatct atcaaaagca gatggcaaaa attgttatag tacatacgaa taataaagct
     5341 atttatacga ccaagaatga acctttaaat ttaatttcat agtctaacaa tcaaagagtt
     5401 gaagctaatc ttatagctag tcaactagtt cctagaaaaa atttaaacaa tatatttatt
     5461 gatactgctc atttttctaa agagcaaatt tatataaaaa attaaacttt tatatacata
     5521 cagatactaa tgataaacag ttatcattag tatcttctat cttaaaacaa aatgataatt
     5581 tatctatata aaaaagtgct ttgttgacag agttcacttt tttatatagg tacagaattt
     5641 aagtataaat acatgtttta ttctatacat tttagttaat gtaaaatttc ttgattgtac
     5701 tctctaactt ttcaacatta tactatttat ataccacttg gttaatataa accccttagc
     5761 tggttctagt gctttttcta tgattttagt catatttaca taccacttag ttaatataaa
     5821 actcattgta ctcgttaagt ttatttgtaa agtagtctaa tttacatacc acttagttaa
     5881 tataaaacca gtggtcgaga cggtctacat tcaaccccgt ttaaatttac ataccactta
     5941 gttaatataa aacgagcaaa cgggtgcggt ctatggtact atacctatgt tatttacata
     6001 ccacttagtt aatataaaac aacgcttccg ctaatagttt cgctatttca ctttcaattt
     6061 acataccact tagttaatat aaaacatcta tactgattaa gctagatttc tttgaggaat
     6121 tatttacata ccacttagtt aatataaaac tattagaagt tgtaacctgt ttaggcttaa
     6181 ttaaaacatt tacataccac ttagttaata taaaacccca aaataaactg ggcattttca
     6241 atacttacac atatgcaact ctcttaaatt tgcagcgaac catgagtagt gcaaatgata
     6301 aagcttatca catgccctca acaccttata tttcaagcgt taagccctac tttgtaacaa
     6361 atatcgctca ctgcaaaact atttacattt ttattatatc ataaatatat tatttttgaa
     6421 tatctgtacc aatttgtggt ataataaaaa caagaagaac tacaatctat ttagacgtag
     6481 agtgaagttc aaatactaat gtttattctt tttgaacttg atttttagtt caaaactaaa
     6541 gtcactcctg ccagagtggc tttttacttt tttaaacaat ttactattta agtaaactat
     6601 taaactggca actaaacttg ctagtacacc ttgtaaaaaa ttatccatac atactcacct
     6661 cccttctata cattgggagg ataatctttt gtatgaactc cactctataa attgtagatt
     6721 acatcttctt gctaaaaatg ttatagcata taattcttac atattttacc tatatattac
     6781 ttttctaaag attcgtctat atatttctgt atacatctac tctatcctcg atttgttaca
     6841 accccaatca aaaaagtagt tcaacttcta agttttatac aaagcaggaa actcaattat
     6901 tattagaaaa atcaaaaaat attcctatag gtataccaat ttttttgatg ctaactcttg
     6961 gattacgttt tggtgaagct gtatcactta tatggtcaga tgtagacctt gataaaaaat
     7021 ctcacatgta aatcaaacat tagtttatgt atacaataaa ataatcttca aagaccctaa
     7081 aacccctata tccaaaagaa aattatttgc tcctgatgaa cttataggtt tgcttaaagg
     7141 ggaaaaatca aaacaaaata agttaaaatt acagggtata ttgaaaaatg aatttgattt
     7201 gatatgctta aataaaagat tcaacccgtg agttcaacca acttttgttg ttcattttaa
     7261 aaaactatta agagataata atcctagaga tataaaagta catgaattaa tacatgcaaa
     7321 tgcaactttg atattacttt caggtactaa cataaaagct atttataaaa tattagtaca
     7381 tgcagatata aaaatagcta tgaatatata ttccatattc tcacttatta gatgaaatgg
     7441 ataaagaaac atctgaaaat ataagcaaaa tactattcaa ataatgttca gttttatgtc
     7501 aattaaatgt cactcaaagt gctttttttc catttgtcag ttaaatgtca cttaaagtac
     7561 ctttttccta tttgtcagtt aaatgtcagt aatatataaa taagatatat ttataagacc
     7621 cttaaaatca ctattattta ataaaattta taattatata ctatttgctg ttgacattgg
     7681 tattatacca tagtttatca tttatattag aataaaatta ttaattaata gtttcgtaat
     7741 tttataatag atttacaggg agtgataata aatggataca aaaatatcta taaacgaaaa
     7801 catgagtcta ggtaaaagat tttttaaata cctagcacca tctgtagttg caatgtgggt
     7861 tttctcacta tatacaatgg ttgatggaat atttgtaagt aaaggagttg gagaattagc
     7921 tttagctgct gtcaatatat caatgccttt tattaatttt atatttgcag tttctctatt
     7981 attttcaact ggtgcatcta ccataattgc aatctatctg gggaaaaaag atattaaatc
     8041 tgctaacgaa gtcttttcat ttaacttagt atcaataatt atattatcaa taattatatt
     8101 agctataact ttttttaatt tagatagatt agcactattt ttaggagcta cagaatctac
     8161 aattggtatg gttaaagatt atttaggaat aataatattt tttaacggat tctttatagt
     8221 gtcttattct ttagaagtta taataaaaac tgatggcttt ccaatattag ctactgttgg
     8281 tgtaataata tctgctctta caaacatcat acttgattac ttgtttgtaa ttgaatttgg
     8341 atggggtgta aaaggtgctg gtattgcaac tggtttgtct caagtctttt ctactatttt
     8401 cttcttaata cattttttaa gaaaaaactc tactttgaat ttttcaaaat ttagaattga
     8461 ttttaagaca cttagaaaaa ttgtatttat aggttttcct gattcaacta ctgaattaag
     8521 ttgcggtata gttgttttac tttttaactt aagtcttact aaatatattg gagaaaatgc
     8581 tcttatttat tatagtgtca taaattatat aaatacatta gtacttatga ctatgatggg
     8641 tataactcaa ggtatgcaac cacttactag cttttattat ggtgcaggaa atatagataa
     8701 tgtaaaaaaa ttattaaaaa tggggataaa agccacaata atagcatcag ttgctgtatt
     8761 tgcaatttgt atggcttttt caggaccaat agtctcttta tttatacatc cagaagaaac
     8821 aatgctattc aatgaaggag ttcgtgtatt taaaatattc tctatatctt tcttattggt
     8881 aggaattaat gtaataattt ctggattctt tgtatcagta gaaaaaccat caatttctac
     8941 tgttatatct cttggtagag ggcttgtaat agttgtttta agtctaattt caatgatact
     9001 aatatttggt ggacaaggaa tatggatgac tactattgtt tctgagttta tatgtcttat
     9061 tttatcttta gtttttctaa aaaagaattt ttctacatta gactctaatc taaataaggt
     9121 tgcttagatg ataagagtag caatatatga aaatatctaa ataaaaaata cctattatac
     9181 aatcttgcaa atattaaacc tttgcaaaaa ttaggattat ataataggta ttttatttat
     9241 gtaattattt tatttctaaa tatccatagc catcttttat taaagttcct tttacttcat
     9301 attctacagg ttttttaaat attggaccat ctacgacaaa tgtatgatac tctccatgtt
     9361 ctccacatgg gtctgcacca gcatttttta tcttctccac aagttctcta gttaattttt
     9421 ttcctaaaaa ttcctctcct aagtgcttta agtttacttt tgttacaaca ctacaaaatc
     9481 cactgtctat aaattcatat actaaatcct ctctactctc ttgccataat ggtaaatcta
     9541 ctttcatacc aactgcttca catctatcag tatcccattt cctatgactt tctatatcta
     9601 tatctccata tgcacatatc gttgctccca tgtttttagc ttttattaaa gccctctcaa
     9661 agctctcttc atatccttct acaccacact cagcaactaa taaaggtaat cctatattgc
     9721 tacttacttg cttaagcata ttatattcta aattatgtgt ccatgacttt ccatgttctt
     9781 cacttatagt tgtcaagagt gccacaggct tatatccttt tttcaacatt cgatttaatg
     9841 ctaatatact atccttacca ccactaaatg acattacaaa tttttcatca gccaatttta
     9901 acacctcttt catctaactt tgttttatta acataattat accaaatttt tgagttgcat
     9961 agcaaattat attaaattgt taattcacta taaagaatgt ccctaatatt aaaagtataa
    10021 cccctatgta tttgtataaa tgcattgttt cttgtcccaa aattacgcta tcaataaaaa
    10081 atccaactaa aacttgggat acaagagtta atataagtgt atttgatact cctaaatttg
    10141 gaattgcctt tactgtaaaa tatattatta gacctccaaa tactcctcct aataacaact
    10201 tataattaaa accatttata gatactagac actttaagtc acctgtaact attatacttg
    10261 ccaaaaaaaa tattgctcct acagttaagc ttataaaggt ggccactaat gctgttgtac
    10321 tttttcctag ctctccattt ataaaagctt caagagctgt agaaccacct gctaataatg
    10381 caaatattat tgcccataat tttgtcatgg acaaacctcc ttctatataa ttctattaat
    10441 agaagtttat gatacaacct attaattaat tactaagtat agtttaattt ttatacacta
    10501 ggctttattt ggatatgaat ttctgttata attgttctat cgattttgga ggtgatttta
    10561 tgtacacaaa ttttaatatc gattttaata actttaataa aaaagaaaat gccattaaat
    10621 ttatgttaat gggagtatta ctcattatat taggattatt atgccttaca tttaagacat
    10681 taggtataaa attaatttct tggacttttg gtattgcact tttattcttt gcatatttaa
    10741 atttaaaaaa tattaatgaa ctaaaaagat atgctactaa agaagaaatt aaaccctcta
    10801 ttaatatcca atgggtttta ataattgcat gtatattact ttttgtattt cctcaaaaaa
    10861 tacaaagcat attttcatta tttttaggat tttatttaat ctttaatcaa ctagtagcac
    10921 ttgtaaatag taagaataat ccttattcta aatttacaac ttggaatata gttaagattc
    10981 tatttggtat atgtcttata ttatctccac tttttttatc tagatttata gtatcgataa
    11041 tgtctttttt cataatatta tttggactag ttctattttt ctctggaaat acagcgagaa
    11101 aatattaatt attttctcgc tgtattttta tattacttaa aatagaattt gtttaagtta
    11161 tctatttgtg gtaatatgtt ttttgtacaa gctcagcgta caattttatt agaaataggt
    11221 gatttttttg gataaattta aatatgcttt tgataataaa agatatcata cttggaatta
    11281 ctatcttaga aatacttttg gagaaaaagt ttttaaggta tctataaatg caggatttac
    11341 ttgcccaaac attgatggaa gtcttggtta tggcggatgt acctattgta gcaaagaagg
    11401 ctcaggtgat tttgctggaa accctaaaga taatcttatc agtcaatttt ataatataaa
    11461 agaaatgatg ttaaaaaaat ggcctcatgc aaagtatatt ggctactttc aagcctatac
    11521 aaatacgtat gctcctcttg aagttttaaa agaaaaatat gagactatat tagagcttga
    11581 agatgtcata ggtctttcaa tatccacaag acctgactgt ttgccagatg atgttgtaga
    11641 atacttatct gaactaaata aaagaaccaa tttatgggtt gaacttggac ttcaaacaat
    11701 tcatgattct acatctaaaa ttataaatag aggtcatgat tataaaacat ttgttgaagg
    11761 tgttgaaaaa ctaaagtcta aaaatataaa ggttgtcgta cacattataa atggtcttcc
    11821 tggtgaagat tataatatga tgatggaaac agcaaaggca gttggtaaaa tgggtgtaga
    11881 cggtataaaa atccatctat tgcatgttat aaaagatact cctatggaaa agatgttaca
    11941 aaatggtatg ttaactctta tggaacaaga tgaatatatc aatcttgtct gtgaccaatt
    12001 agaaatactt cctgaaacta tgataatcca tagactcact ggtgatggta agagggatga
    12061 actagtaggt cctatatgga gcctaaaaaa atgggaaatt ttaaatcaaa ttgatgatac
    12121 tttaaaggct agaaattctt atcagggctg taagtttgtt taatacaaaa taataactat
    12181 agaaatggtt atataaatat agtcctttct atagttattt agtatttaca aaacaaaata
    12241 ttaatttgta catttataca atatataata aaaaatagat agtctaaact gtatctactt
    12301 atattttatt tgtactgtcc actttattct ataaaattta taaatagtta ttttttaata
    12361 ccaaatataa tttggtataa tttttgttgg actctaaaat gtataccaat aaatcctttt
    12421 taagtatata attctaaata ctttcagttt tttcaatctg ctttaacttg ttggacttat
    12481 aaaatattct tatttcatca taagaaagat taagtgctac tactgcagat attaacatca
    12541 acatcactcc aaataacttc cctaaggaaa aactttctcc taatatagtg caaccaatta
    12601 ttactgaacc aactaattct actgatgcca caacacctgc aactgacaac tctacacctt
    12661 tagatatacc tgctgcataa aaaatatatg caagtgcagc tggaacaatg cctaacatta
    12721 gcatacaaga taaaacatct aaatccttag cataatttaa gatttcccaa ggtcttgaag
    12781 aaggaatcat aaatatagct ccaaataaga aactgtatac taatattgtt gaacttgaaa
    12841 attcctttaa agcattttta ctaatagttg gcataagtgc atatgctatt gctgataata
    12901 ccccaagtaa aagtccaaaa gcgtttaaac cttgaaaatc caaacgtcca ccagtcactg
    12961 ccataattgc accaataaaa caaagaataa gtgaaaataa tttgcttcta gtaatatttt
    13021 ctttataaca tacttttgaa aatattgcta aaaacagtgg tgatgtatat agtaaaactg
    13081 ctgcaattga tacacccgca atgtcaattg ctttaaaata acataaatta aacattgctt
    13141 gacatataat acctattatt actgaatata taattccttt tttgcttatc tttaagattt
    13201 gtggtgtcct tatagatgaa tatacgccta aaactataaa tccaaaaaac aatcttgtaa
    13261 atgctacttg ttctgatgtc agtccatttc ccattaaaaa ctttccaaat aatccaagtg
    13321 tagcccataa taaccctgct attgctataa atacataccc tttacttttt tctcccattt
    13381 taaattccct tctattttta tttagtcaat ttacactctt ctttttaagc aaaaactatg
    13441 ccatcttaaa tcaatatata tcgactctaa aaccctagtc tttccaataa tactaataaa
    13501 ttttcaattt ttttatattt atttttcgct tactttaagt tgatttagac ttattttaat
    13561 tgtaaaatga gttatatata acttatttaa agttaaaata gactgaaact tatata
//
LOCUS       DAEMHZ010000114        13584 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000114 DAEMHZ010000000
VERSION     DAEMHZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.114"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            160..2661
                     /locus_tag="KPK72_002916"
     CDS             160..2661
                     /locus_tag="KPK72_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG2490113.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKVAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIRLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2821..3489
                     /locus_tag="KPK72_002917"
     CDS             2821..3489
                     /locus_tag="KPK72_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490114.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3473..4750
                     /locus_tag="KPK72_002918"
     CDS             3473..4750
                     /locus_tag="KPK72_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG2490115.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5151..5372
                     /locus_tag="KPK72_002919"
     CDS             5151..5372
                     /locus_tag="KPK72_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490116.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5661..6425)
                     /locus_tag="KPK72_002920"
     CDS             complement(5661..6425)
                     /locus_tag="KPK72_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2490117.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6699..7373
                     /gene="fsa"
                     /locus_tag="KPK72_002921"
     CDS             6699..7373
                     /gene="fsa"
                     /locus_tag="KPK72_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2490118.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7495..8889)
                     /locus_tag="KPK72_002922"
     CDS             complement(7495..8889)
                     /locus_tag="KPK72_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG2490119.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(8967..10313)
                     /locus_tag="KPK72_002923"
     CDS             complement(8967..10313)
                     /locus_tag="KPK72_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2490120.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      10563..10665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10759..12135
                     /locus_tag="KPK72_002924"
     CDS             10759..12135
                     /locus_tag="KPK72_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2490121.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(12249..13394)
                     /locus_tag="KPK72_002925"
     CDS             complement(12249..13394)
                     /locus_tag="KPK72_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2490122.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
BASE COUNT         5235 a         1630 c         2104 g         4615 t
ORIGIN      
        1 actaatacat ttatttatta aaaaaatatt gatttaatat acctataggg ggtataataa
       61 aaatatagat ttctaaataa gtaatttatc aatattacat atcaaaataa aacgttacaa
      121 attagctgaa aatatattga aagaaaggaa gattttatta tgagttcaaa taaagtggta
      181 gaaagttata aaattacagg tatgacatgt gcagcttgtg caaaggcagt agagagagta
      241 actaagaaga tggatggagt atatgaccaa agtgtaaata tagctactga aaaacttaaa
      301 atagaatatg ataattctaa ggttaatttt gatgatataa agcaggttgt agaaaaagca
      361 ggatatggaa ttataaagga agaaagtaat aaaaaaattg atatgaaaat tgatggaatg
      421 acatgtgcag cttgtgcaaa agctgtagag agagtggtaa aaaaattgga tggagtagaa
      481 agtattagtg tgaatattgc aacagataaa gctaatatag actatgaccc atcaaaagta
      541 aaattatctc aaataaaagt agctattgaa aaagctggat ataaaccaat agaagaggta
      601 agaaataagg ttgatgtgga tgaagataaa ttgagaaaag aaagagaaat gaagtcttta
      661 tttgttaaat ttatagttgc aatagtattt gcagtacctc tgttttatat agcaatggga
      721 cctatgataa taaagcctat agggccatgg cctttgccag aaattataaa tcctatgaca
      781 aatactttta attatgcatt aatccagtta attttagtaa taccagtaat gatagctggt
      841 tataaatttt atataaatgg gtttaaatca cttttttctt taagtcctaa tatggattct
      901 ttagttgcaa taggaacatt agccgcattt ttatatagtc tatatacgac actacaaata
      961 gccaatggac aaattcaagg tatgcaccat catcagttgt attatgaaag tgcaggaata
     1021 ataatagcac ttatcttact tggtaaatat ttagaatcaa aatcaaaagg aaaaacatca
     1081 gaagcaatta aaaagcttat ggggctacaa cctaaaactg caatagtgtt agttgatggt
     1141 aaagaagtag aaactcctat agaagaagta gagataggag atatactatt agtaaaacca
     1201 ggaactaaaa tacccgtgga tggagttgta atagaaggat atacatcagt tgatgaatca
     1261 atgcttacag gtgaaagtat accagtagaa aagaatgttg gaagtaaggt gacaggagct
     1321 agtattaata aaaatggtgt tattaagttt aaggctgaaa aaattggtgg agatactgct
     1381 cttgcccaaa taataaagtt agttgaagat gcacaaggta caaaagcacc tattgctaaa
     1441 cttgccgata ctgtatctgg atattttgta ccaatagtaa ttgcaatagc tgtagttgca
     1501 tcattattgt ggtttttaat aggaggaaaa gatatagtat ttgtacttac tatatttata
     1561 tcagtacttg taattgcttg tccttgtgca ttaggattag caacaccaac tgctataatg
     1621 gttgggactg gaaagggcgc agaaaatgga atcttgataa aaggtggaga ggcactagaa
     1681 tctgcacata aagtaaatac agtgatattt gataagacag gtactataac agaaggaaag
     1741 ccaaaagtaa cagatatagt attaaataat aatgtaaaag aagaatatct tatcaagatt
     1801 gcttcaagtg cagaaaaagg ttcagaacat cctcttggag aagcaatagt taagtatgga
     1861 gaagagaaga atataaaatt tgaaaaagtt gataatttta aagctatacc aggtgctggt
     1921 atacaagtta ctattaatga tgaaagtata ttacttggaa atagaaagct tatgaatgac
     1981 aataatataa agcttgggga tttagaagaa aaatcaaata tattggcaag ccaaggtaaa
     2041 acacctatgt atatagctgt agatggaaat ttatcaggta taatagcagt tgcagatgta
     2101 gtcaaagaaa gtagtaaaaa agcaatagaa atacttcatg atatgggaat aaaagttgca
     2161 atggtaactg gagataatgc gaaaactgca aatgctatag caaatcaagt cggtatagat
     2221 atggttttgg cagaggtttt accagaagat aagtcaaaag aagttgaaaa actacaaaat
     2281 caaggaaagt ttgttgcaat ggtaggggat ggaataaatg atgccccagc tcttgcaaaa
     2341 gcagatatag ggatagcaat aggaagtgga acggatgtag ccattgaatc tgcggatata
     2401 gtacttatga agagtgattt aattgatgtt ccaacagcaa ttagacttag tcatgaaact
     2461 ataaaaaata taaaacaaaa cctattttgg gcatttggat ataatacaat tggtatacct
     2521 gtagcagcag gaatattata tgtatttgga ggacctttat taaacccaat gattgcagca
     2581 gcagcaatga gcttaagttc agtttcagtc gtatcaaatg cacttagact taaaaacttc
     2641 aaggcatata agagagatta gattaataaa ttaaataaaa tgtatccaaa agagctatct
     2701 ttaaattaaa aggattaatt tacaaattaa gatagctctt ttttcatgta aaattcacaa
     2761 caataagata aaatataagt aatatacgaa attttaaata aaatagaaag gattgataga
     2821 atgaattcgt caatacttgt aattgaagat gattcaaata tacaagagtt aatatcagaa
     2881 tttttaagtg cagaaggtta tcaagttgat acagcaaatg atgggttgga aggtatacaa
     2941 aagtttaagc aaggaagtta tgatttagta atattagata taatgatgcc aaacttagat
     3001 ggttatggag tttgtaaaat gataagaaag tcatcaagcg ttccaattat atttttaact
     3061 gctttaaatg atgaaggaga ccaacttaaa gggtttgact tagaatgtga tgattatata
     3121 acaaaaccat tttcatttaa tcttcttata aaaagagtag aagcaatttt aaggcgaagt
     3181 aacaagacta taaatgacaa atttatagtt tttgaaaaat taaaattaga tttaaacaca
     3241 tatattgcag agatagatgg agaacctata gaacttacac taaaagagtt taatatatta
     3301 aaagctttga tagaaaagta tccacaagtt ataactaggg aaggtctttt ggatagtata
     3361 tggggttatg actattatgg agatactagg attgttgatg cacatataaa aaatataaga
     3421 aaaaaaatat cattaccata tattaaaact gtaaaaggaa taggatatac acttgagaag
     3481 gatatttgat aagtgggaaa agttaagtat aaagtataag ttattttcaa taacgacatc
     3541 attattaata gcattagctt taataatata tctaatttta tattttttat tgccatcata
     3601 ttatcatgag tataaaattg agtctcttca agaaagtttg aaatctttag tagatagttc
     3661 aattcatttc gatacatata ctttagaaga aagactttat tatatggcta aagaccaaaa
     3721 tttggctatt ttattaaaag ataaccaagg taaaattgta tatggtaaga atgaagtagt
     3781 tattttaaga tatagtaagt acatgattaa tagtcttgag gatgagtata gaacttctat
     3841 acctatatat actaaagatg caaaagatgg tccatatact ttagaactcg ttatgccatt
     3901 acaaccaatt gacgaagcaa acgaagttat aagaaaacta atgccataca tcatatcaat
     3961 agcaatattg atagcgatta ttggagcata tatatattca attgtaataa caaagcctct
     4021 tataaacata atagagagtg agagagaaca ggaatataga aggaaggatt ttgttgctac
     4081 aatatcacat gaactaaaaa ctccaataac cataataagt ggtcaaattg aaggtatgat
     4141 ttatagtgta ggtaagtata aggataggga tacttatctg aaaaaatcgt atgagtgtac
     4201 acaagaatta aaagatttag taaatgaaat gatagaggtt tcaaaatcag aaatacttga
     4261 aaaagatttg aaattggtat caataaatat aagtgaatta ttaaatagac ttgtaaagag
     4321 acaagtattt ttaatagaag aaaaacatat gaaaactatt ttaaaaatag aagaaaattt
     4381 ggaaattaag gcagaccaag agagaataac taaggcaatc aataatataa taaataatgc
     4441 cataaaatat tctcctgaag aatcagagct tataattaga ctctatgata aaaataaacg
     4501 aataagtaaa aagacttcta atcaaagagt ggttttagaa attgaaaata ctggtgtaac
     4561 tatcgagaaa cgttatttag aagaaatatt taatccattt tatagaatag aaaaatctcg
     4621 ttcaagaaaa actggaggaa gtggacttgg tctttatata gtcagccaaa tttttaaaag
     4681 tcatgggttt gattatagca taaaaaacaa agaaaactct gtagtattca cagtagaatt
     4741 taaaaattaa ttacttatta taaagaatat ataactttca ataatagttc attttaaaat
     4801 cacaaaaaat tcataatgaa actgtattat ataaatataa agaagcttaa aaccataaaa
     4861 tacaccaact ataatgatga gatttcctta catcctccat ataagtcatc attatagtaa
     4921 cccaacaatt aagttactat gaatttaaaa aataacaccg agcatataat ctatgcttag
     4981 tgttattttt attttgaaat aaaaaatgtt taatttattg aaatgtatag atttagatag
     5041 tttaaactat atttaagact taaaaattat aattgatatt acaaataaat atatgtactt
     5101 tgggaaaaat aaatatagga agtattatat ttacagagga ggtagttttg atggatagat
     5161 taaaaagaga tatgaaaaat atgaaagaag atttaaaaga aggtgttaaa aagacagctt
     5221 atgttatgaa aaatacagct gaagatttga aagatgatgc acaaggtgct atggtaaact
     5281 tagaaatgaa aaaagatgag atgatggaaa gatatgaaca aaagaaattt tctaaagaat
     5341 tagagaagca aatgaagaat gaaatgaaat aagcttttat ttatgaataa caaattattg
     5401 tattcatata aaaagtagcc agtaatatta aatagtttat ttaaggaaat agactttcta
     5461 aaaataactg tattagattt agaaagtcta tttcaaatta gttttataaa tagacataag
     5521 gataaagtag atgttataga actagatttt aaaaaataga tactaaggat aaaataaata
     5581 ttatagaaat atgtttttaa gaatatttat caaataaaaa tttatattta taagaaatcc
     5641 tataaaacat ataattaaac ttataaaaca tttacattaa attctgataa aatatctact
     5701 atatcaggag taggcgtttt ttctgtaatt atagaactaa tatcttctaa atgagcaaac
     5761 ttgaaatttc catcaaattt aaacttttta tcttccataa ctataaatac ttttttactt
     5821 gaatttatta tagccttctt cgtaagacca tcttcaattt caaaagttgt aagtgaagag
     5881 ttgaatacat ctactccaca acttcccata aaagtcttat caaacttata tttcgatata
     5941 aactctatag ttggagtacc gacaaaaccg tctaaactga gatttaataa acctccagtg
     6001 gaaattatat ttaaattatt tggttttgaa gtaactacat ttagtatatc cagcatattt
     6061 gtaacaagtg ttactttttt attactttca gctaacaact tcgcaagcaa gatgttaatt
     6121 gtagagatat ctaaaaatat agtttctcta tcttcaatta aattaaatgc tttttctgct
     6181 attattttct ttgttggaat attaatttct tttctgtctt ttgtatcctg attttctagt
     6241 gtttctctag ctagtacagc tcctccataa attcttctta ttgaagaaat cttttctata
     6301 ttttttaaat cttttcttat acaatcttct gtaacattaa atttagaact gagctctttg
     6361 acagtaactc taccttcttt ttttaggata tttaatatag cttggattct ttcttctgca
     6421 aacattttct acctcttttc gcaatatagt attatagtaa atattataag ataaaataca
     6481 atatatcaac tattttaaca tgaaatacaa aattaattta atataaaaag tatgtttttt
     6541 ctaactatat atttagaatt tagtaaataa aaaaattata aaactaagaa ggaaaagtaa
     6601 aacaataata aaaaataaaa aacaataagc aaaatgttga aaataatacc aaatcaatat
     6661 ataataagaa taaagaaaga ttaaaggggg atattttaat gcaactaatc ttagatacag
     6721 gtaatgtgga ggagattaag gagttatgta cttgtttacc aatagatggt gtaactacaa
     6781 atccaagtat tgtttcaaaa gaaaaaaaga attttaagca acttataaat gaaattggtg
     6841 agattatagg tgaggatatg cctatacatg ctcaagtgtt aagtacaaag tatgaagaaa
     6901 tattagaaga agcattatat ataagtagtt tgagaaaaaa tatatatgta aaaatacctg
     6961 taacacaaga tggtttaaga gctataaaag accttcacag aaaaggtgta aaaataactg
     7021 ctactgcaat attcactgct catcaaggtt ttttagctgc aaaggctgga gcaaattata
     7081 ttgcacctta tgtaaatagg cttgataata tatctggtga tggagtagct atggtttcag
     7141 aacttataaa aataatagat acttataaaa tggatacaaa agtacttgca gccagtttta
     7201 aaaatgcaca acaagttata gaattaatgc aacatggggt gcatagtgtt acagtaccat
     7261 atgatatttg taaatctatg atgaatcatc cattaactga ttggagtgta gataagttta
     7321 tagaggattg ggagaatact tttggaaaag gaagtaaaac aaataatatt tagtattatg
     7381 gtaaaagagt agttttgtcc aatttcacaa gaacttatat aaaaagatga aaatatgaat
     7441 tttcttttta ataatttcgt tattatctta agttaattca tattttcatc ttttttagat
     7501 ttctagtcct ataaatttcc accaagggaa tgttatacat attattataa agtaggttaa
     7561 aggaatcaaa gttaaaccgt atttgaaaat aaccttgcta ttatacagat tttcaccatg
     7621 accaaccatt attgtagcat gatgaaatgg taatatgtac tgtatgctta ctataatgta
     7681 gacaaataga cataaagtaa cactattttc ctttagtata tgtaaacttc caagtaatgg
     7741 tattactaca gatgatgtag tgacagcact tccaagacat atattcaaga ttattgtgag
     7801 agttatcaaa aacaatattg aaataatttc tgaagagttt ggtataaact ttatcaaata
     7861 atttcccatt atatctacaa ctccagagta ctttaacact cctccaattg aaaatgctgc
     7921 tataaaaaaa ataaggacct tccaatttat ttcttttaaa gtactaaatc caactatttt
     7981 tcttagtaat aaaagaagta cacaaattga cattataatt aatgtgttta tgttatgtat
     8041 gaactgagtt aaaaacataa aaaacattat tcctaggcat aaaaatacgt gtttttgctc
     8101 tttgtttagc aaattaaatt ctttttttaa attagaatat gaaaaatgaa gtttattacc
     8161 ctcaagattt tcattagaaa taaattttat tgaatgcaat tcttttttaa agacaaagat
     8221 aaataaaaaa taagttataa aacaagtaat tatagttgga acagacatat aaaaagccca
     8281 ttgaccccaa tttatattac aatttccaag ttctagtaca acataattta gaagagtgtc
     8341 tccatttaca aaaaacattg atgtaaaggt agatgctgta aatatactaa ataaaagtat
     8401 agattttgaa tcttcagtta tctgttgttc ttttaaaaac tccttgtaaa aagcagatac
     8461 aagaataacc cttggaaatg gttgtgggat aaaaaatatt aaaagtatac ctaaaatata
     8521 cgataaaaga atcatttttt taggggtatt tacagtattt aaaattaact tctcagaaat
     8581 aatttttgcg acacctgttt ttgtaactgc attagtaatt atgtatgcaa gaattattat
     8641 gtaaaaattt tctgtaaata aaaactgtaa aataaccttt attggtgcaa tgttaaatat
     8701 aaacgctaga attacaaaaa acaatgcaac aaaagttttt tcaactgagt ctacagccca
     8761 tatcaagata gttaaaaaag ataagcatat agaaaatttt atgttgaaac tgtaattaag
     8821 agagaagtag agaattatgg tagctataac agtcatataa aattgttttg gcttaagaat
     8881 aatacccatt tttatattca cctccataat ttattatatc ataatggagg tgaatacaat
     8941 gaatgtgaat acattttata aaaagattat tcattgattc tatgatttac aatagcttta
     9001 gttttccaat gtcctttctt atagtagata actgtaacaa taagacctaa agtccaacca
     9061 aaaggaatac ccatccaaat tccgtctgtt ccaaaaaatt tagaaagcat ataagaagct
     9121 ggaagtcttc caagccataa agatactatt gagcttataa gtgcaaacat ggaatcacca
     9181 gcacctttca ttgcacttga aagtacaaaa gaagttccaa taaataaaaa gaatggtcct
     9241 attataaata agtaagaaga gccaatttta atgacatctt tatcagtatt gaatagagca
     9301 attagatttt ccctaaatag caaaattaaa gcaataactg taactgctaa aactaaggtc
     9361 atttttatag attctcgaat gcctttttta actctttcat tttctccagc acctatgttt
     9421 tgagcaacaa aagtagaaac agcagaacca agattcataa taggtaagct tataaaattt
     9481 tctatcctaa gaccagcacc aaaagctgcc atagcagatg taccataact attaactaaa
     9541 aattgaagag ccatatttcc aattgaaaac aacatttgtt gaactccact tggaagtcct
     9601 aatctaagtc catctttaaa tattttgtta tcatatacaa ttcctttgat tttaaattta
     9661 agcacttcat gttttttatt taaataaatt atacttatta taaatgatac tgcctgagcc
     9721 attatagtgg caagtgcaac gccctcaaca cccattctta gcactacaat aaaagttaag
     9781 tctaaaacta tatttagaat tgtggctatt atcagaaaat aaagtggagt tactgaatct
     9841 ccaagtcctc taagtattgc acttactgaa ttatatccag cagaaaataa tattccacta
     9901 aatattatag taagatacaa ttttgattga gcaaaaacgg attctggtgt attcataagc
     9961 ttaagcatgg ggccaccaaa tactattcca agaatactta ttaatataga accaataaat
    10021 aaaagtatat atgtagtatc aatagtagct tttacctttt ttaaatcccc tgagccataa
    10081 aattgtgaca ctaaaataga aaatccaagg ctaacaccca taagcgctgc caccaataaa
    10141 aacattatag ggttagctcc agagactgcc gccattgctt cttttccgat aaatctacca
    10201 actattatac tgtcagctgt attgtatagt tgttgaaata aactaccaat aagcataggc
    10261 atggcaaaaa agaatatgga ctttccctca tgacctgtag ttaaatcttt caccttattc
    10321 ctccaaataa aaaaattatt aatttattat agtataatct taacataaac tttatatcca
    10381 aactataaat aaattgtatc aaaatatcat atattttttt aaaatgtaaa gaatataatt
    10441 ataatttgaa tttaataaat caatatttaa tataatcgaa tattaataca aaatcaatac
    10501 aatatatacg tatatattga caaaattaat tatataaatt aaaataatat tcgtaaagaa
    10561 aaattcatgt tttgtactgt ataacatata attttgacaa tatgggtcat aagtttctac
    10621 cggaataccg taaatattct gactatgtat actattattt agttaattaa atatttaatt
    10681 aactgaatta tgaaggatac ggctcagctt atcaactact gtgtcgtttt tttgtaaaat
    10741 ttaataggag gtgttgttat gcaaacaaca aacagtagta ctatgcagaa actttttcca
    10801 attttgacta ataaaaatgt cgacatgaag aaggaaataa ttgcaggagt aactactttt
    10861 ttaactatgg cttatataat agctgtaaat ccaaatattt tatctgagac aggcatgcct
    10921 gcaggtgcac ttgttacagg tacttgttta gcagcagcat ttggatgtat tttaatgggt
    10981 gtagttgcta accttccttt tgctttagca tcaggtatgg gtcttaatgc attttttgca
    11041 tatacagtag ttttacaaat gggtgttcct tgggaagtag ctcttacagc agtatttgta
    11101 gaaggtatta tttttatagt attatcagta agtggagttc gtgaggctgt tgtaaatgcg
    11161 ataccaaaaa atatgaaatt agctgttaca ggtggtatag gaatatttat tgcacttata
    11221 ggtctagtta acagtggaat tgttattgga gaccaagcca ctcttatcaa aatgggtaga
    11281 tttacaccag cagtaataat aacttgtgta ggattaataa ttattgctgt attagataaa
    11341 aaaagagtta aaggttctat actttttgga atagtagtga gttcattact tgcttgggca
    11401 tttgctttta taaatccaga gtatgcacaa aaattaggta tatatttacc tggtggatta
    11461 tttaaattcg aaagtttagc accaatagca ggtaaaatag atttaggata tgtactacat
    11521 ccaacaaata taggtggttt tttagtagta gtttgtacat ttttatttgt tgattttttt
    11581 gatacagtag gtacattagt tggtgtttgt tctaaagcaa acatgttaga tgaaaaaggg
    11641 aatgttccta atgtaggtag agccttactt acagatgcgg ttgcaactac agttggtgca
    11701 ggacttggag tatctacagt tacaacttat gttgagagtt caacaggagt tattgcaggt
    11761 ggaagaactg gatggacagc aataacagtt ggaattttat tcttagctgc aatgtttttc
    11821 tctcctgtat ttatagcaat tccatcatgt gctacagcac cagctttaat atatgttgga
    11881 tatttaatgc ttggtacagt taaggatata gagtttgata atataactga aggtgttcca
    11941 gcttttgtta ctattgcttg tatggcatta acatatagta taggtgatgg tttaacttta
    12001 ggtatattaa cttatgtttt cgtaaatata ttttataata tatttggagc taaaaaggca
    12061 gaagacaaaa aacatgtttc tgtagtaatg attgttctgg caatattatt tataataaaa
    12121 ctttggctac tataataggt attatttaaa attttaattt aatataaaat aaagggctgt
    12181 aatttattat caattgtgat gataaactac agctcttttg tatttgttgt atttttgtat
    12241 ttattgtatt atttaataat gtctattagt tctccatttt gtaaagtttt aggtgtaatt
    12301 actatattga actctttttg caaatctttt ataatatcta attctttttt tgttattatt
    12361 gagattgtgt taccacttcg atttcctcta gcagttcgac cacatctatg caggtattca
    12421 tttttacttt ttggaaaatc caagttaaaa acatgactta catccacaat atctagtcca
    12481 cgagcagata aatcagttgt aattaaaatc tttgctttac caagtttaaa tttatttatt
    12541 gcattctttc tatcttcttt ttccatattt ccaaaaattc ctattgcttt gtaattatga
    12601 taatttagtt ttgatactag gacttctata tttttttcat tatttacaaa tacaatagct
    12661 ttttttggat ttgtagctgc taatgccttt ctaagaaaag taaatttatc ccttatctca
    12721 cctaataaat atgagtgatt gatgttagga tttatttgtg atttttcctt tgtttttatt
    12781 atttcaaact ccttcataag tttatcacaa atttttatag tgctatcagt aagacttgca
    12841 gagcatccta taatttgtct atctcttaac gttgttctta ttatgtcttc tatacaagtt
    12901 atattttttc cattcaatag attgtccact tcatctaaga caatagtttt tatattatgt
    12961 gattttagtt tcttttgttt tatcaagtct aaaactcttc cacaagagcc aattactatg
    13021 tgtggtttta ctgcttttat attttttatt tgtttttgta tattgacctc cccaattaaa
    13081 gctaaagaag taacggagag ttctgcattt ttagccaata attcaacctg gtttgtaatc
    13141 tgcattacta attcatgcgt aggtgcaagt ataagagctt gtgtttctct ttttgatgta
    13201 tcaatttttt caaatatagg taaaaggtac gctagtgttt ttcctgttcc agtctgtgaa
    13261 ttaatcaata aatccttatt ttgaattata ttccctataa caagtgactg aacttctgtt
    13321 ggagaagtta tgtcttgttt tttgagtcca tctattagag tggaacttat ttttaattgt
    13381 tcaaatgtat tcatatttta ctccttatat tttaataatt atagtcaact attttttatt
    13441 atacattgtt tattcaaatc gaactatata ttcttttata attatgtgta atttgaaagt
    13501 aaatatcaat tatatttata cctttatatt tttgatacct ttgcatttta tagatttcta
    13561 aaataaacaa ataaaagttg taat
//
LOCUS       DAEMHZ010000115        13559 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000115 DAEMHZ010000000
VERSION     DAEMHZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.115"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..690
                     /gene="tcdR"
                     /locus_tag="KPK72_002926"
     CDS             136..690
                     /gene="tcdR"
                     /locus_tag="KPK72_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG2490123.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1008..8108
                     /gene="tcdB"
                     /locus_tag="KPK72_002927"
                     /gene_synonym="toxB"
     CDS             1008..8108
                     /gene="tcdB"
                     /locus_tag="KPK72_002927"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG2490124.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGSTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTRETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDTNYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8230..8730
                     /gene="tcdE"
                     /locus_tag="KPK72_002928"
     CDS             8230..8730
                     /gene="tcdE"
                     /locus_tag="KPK72_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG2490125.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8809..8970
                     /locus_tag="KPK72_002929"
     CDS             8809..8970
                     /locus_tag="KPK72_002929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490126.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9059..9241
                     /locus_tag="KPK72_002930"
     CDS             9059..9241
                     /locus_tag="KPK72_002930"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490127.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9458..>13559
                     /gene="tcdA"
                     /locus_tag="KPK72_002931"
                     /gene_synonym="toxA"
     CDS             9458..>13559
                     /gene="tcdA"
                     /locus_tag="KPK72_002931"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG2490128.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKDSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFEGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGN"
BASE COUNT         5478 a         1415 c         2163 g         4503 t
ORIGIN      
        1 atgtattcat aatgcatatt ttcatataaa atttaattta tttgccgatt atataattat
       61 aatgactgat ttaattccga tgttgtcaaa attttcaaat aaatcatcat tataaatata
      121 agagaggatg attttatgca aaagtctttt tatgaattaa ttgttttagc aagaaataac
      181 tcagtagatg atttgcaaga aattttattt atgtttaagc cattagtaaa aaaacttagt
      241 agagttttac attatgaaga gggagaaaca gatttaataa tattttttat tgaattaata
      301 aaaaatatta aattaagtag cttttcagaa aaaagcgatg ctattatagt caaatatatt
      361 cataaatcat tactgaataa gacttttgag ttgtctagaa gatattctaa aatgaagttt
      421 aattttgtag aatttgatga aaatatctta aatatgaaaa ataattatca aagtaagtct
      481 gtttttgagg aagatatttg ttttttcgaa tatattttga aagaattatc tggtattcaa
      541 agaaaagtta ttttttataa atatttaaaa ggatattctg atagagaaat atcagtgaaa
      601 ttaaaaatat ctagacaagc tgttaataag gctaaaaata gagcatttaa aaaaataaaa
      661 aaagactatg aaaattattt taacttgtaa ttaatgagct taaagaaata tttacaatag
      721 aaatcaaatt ttagaattaa ctttattgta aaatcaataa cttaatctaa gaatatctta
      781 atttttatat tttatataga acaaagttta catatttatt tcagacaacg tctttattca
      841 atcgaagagc aaattaatca actgagtgtc ttcaatttaa aatgttagga agtgaatgta
      901 tatgaaaacc taagtagata ttagtatatt ttataaatag aaaggaggat atataaaaga
      961 gttttagcat ttagatgtaa aaatattata gtaaaggaga aaattttatg agtttagtta
     1021 atagaaaaca gttagaaaaa atggcaaatg taagatttcg tactcaagaa gatgaatatg
     1081 ttgcaatatt ggatgcttta gaagaatatc ataatatgtc agagaatact gtagtcgaaa
     1141 aatatttaaa attaaaagat ataaatagtt taacagatat ttatatagat acatataaaa
     1201 aatctggtag aaataaagcc ttaaaaaaat ttaaggaata tctagttaca gaagtattag
     1261 agctaaagaa taataattta actccagttg agaaaaattt acattttgtt tggattggag
     1321 gtcaaataaa tgacactgct attaattata taaatcaatg gaaagatgta aatagtgatt
     1381 ataatgttaa tgttttttat gatagtaatg catttttgat aaacacattg aaaaaaactg
     1441 tagtagaatc agcaataaat gatacacttg aatcatttag agaaaactta aatgacccta
     1501 gatttgacta taataaattc ttcagaaaac gtatggaaat aatttatgat aaacagaaaa
     1561 atttcataaa ctactataaa gctcaaagag aagaaaatcc tgaacttata attgatgata
     1621 ttgtaaagac atatctttca aatgagtatt caaaggagat agatgaactt aatacctata
     1681 ttgaagaatc cttaaataaa attacacaga atagtggaaa tgatgttaga aactttgaag
     1741 aatttaaaaa tggagagtca ttcaacttat atgaacaaga gttggtagaa aggtggaatt
     1801 tagctgctgc ttctgacata ttaagaatat ctgcattaaa agaaattggt ggtatgtatt
     1861 tagatgttga tatgttacca ggaatacaac cagacttatt tgagtctata gagaaaccta
     1921 gttcagtaac agtggatttt tgggaaatga caaagttaga agctataatg aaatacaaag
     1981 aatatatacc agaatatacc tcagaacatt ttgacatgtt agacgaagaa gttcaaagta
     2041 gttttgaatc tgttctagct tctaagtcag ataaatcaga aatattctca tcacttggtg
     2101 atatggaggc atcaccacta gaagttaaaa ttgcatttaa tagtaagggt attataaatc
     2161 aagggctaat ttctgtgaaa gactcatatt gtagcaattt aatagtaaaa caaatcgaga
     2221 atagatataa aatattgaat aatagtttaa atccagctat tagcgaggat aatgatttta
     2281 atactacaac gaataccttt attgatagta taatggctga agctaatgca gataatggta
     2341 gatttatgat ggaactagga aagtatttaa gagttggttt cttcccagat gttaaaacta
     2401 ctattaactt aagtggccct gaagcatatg cggcagctta tcaagattta ttaatgttta
     2461 aagaaggcag tatgaatatc catttgatag aagctgattt aagaaacttt gaaatctcta
     2521 aaactaatat ttctcaatca actgaacaag aaatggctag cttatggtca tttgacgatg
     2581 caagagctaa agctcaattt gaagaatata aaaggaatta ttttgaaggt tctcttggtg
     2641 aagatgataa tcttgatttt tctcaaaata tagtagttga caaggagtat cttttagaaa
     2701 aaatatcttc attagcaaga agttcagaga gaggatatat acactatatt gttcagttac
     2761 aaggagataa aattagttat gaagcagcat gtaacttatt tgcaaagact ccttatgata
     2821 gtgtactatt tcagaaaaat atagaagatt cagaaattgc atattattat aatcctggag
     2881 atggtgaaat acaagaaata gacaagtata aaattccaag tataatttct gatagaccta
     2941 agattaaatt aacatttatt ggtcatggta aagatgaatt taatactgat atatttgcag
     3001 gttttgatgt agattcatta tccacagaaa tagaagcagc aatagattta gctaaagagg
     3061 atatttctcc taagtcaata gaaataaatt tattaggatg taatatgttt agctactcta
     3121 tcaacgtaga ggagacttat cctggaaaat tattacttaa agttaaagat aaaatatcag
     3181 aattaatgcc atctataagt caagactcta ttatagtaag tgcaaatcaa tatgaagtta
     3241 gaataaatag tgaaggaaga agagaattat tggatcattc tggtgaatgg ataaataaag
     3301 aagaaagtat tataaaggat atttcatcaa aagaatatat atcatttaat cctaaagaaa
     3361 ataaaattac agtaaaatct aaaaatttac ctgaattatc tacattatta caagaaatta
     3421 gaaataattc taattcaagt gatattgaac tagaagaaaa agtaatgtta acagaatgtg
     3481 agataaatgt tatttcaaat atagatacgc aaattgttga ggaaaggatt gaagaagcta
     3541 agaatttaac ttctgactct attaattata taaaagatga atttaaacta atagaatcta
     3601 tttctgatgc actatgtgac ttaaaacaac agaatgaatt agaagattct cattttatat
     3661 cttttgagga catatcagag actgatgagg gatttagtat aagatttatt aataaagaaa
     3721 ctggagaatc tatatttgta gaaactgaaa aaacaatatt ctctgaatat gctaatcata
     3781 taactgaaga gatttctaag ataaaaggta ctatatttga tactgtaaat ggtaagttag
     3841 taaaaaaagt aaatttagat actacacacg aagtaaatac tttaaatgct gcatttttta
     3901 tacaatcatt aatagaatat aatagttcta aagaatctct tagtaattta agtgtagcaa
     3961 tgaaagttca agtttacgct caattattta gtactggttt aaatactatt acagatgcag
     4021 ccaaagttgt tgaattagta tcaactgcat tagatgaaac tatagactta cttcctacat
     4081 tatctgaagg attacctata attgcaacta ttatagatgg tgtaagttta ggtgcagcaa
     4141 tcaaagagct aagtgaaacg agtgacccat tattaagaca agaaatagaa gctaagatag
     4201 gtataatggc agtaaattta acaacagcta caactgcaat cattacttca tctttgggga
     4261 tagctagtgg atttagtata cttttagttc ctttagcagg aatttcagca ggtataccaa
     4321 gcttagtaaa caatgaactt gtacttcgag ataaggcaac aaaggttgta gattatttta
     4381 aacatgtttc attagttgaa actgaaggag tatttacttt attagatgat aaaataatga
     4441 tgccacaaga tgatttagtg atatcagaaa tagattttaa taataattca atagttttag
     4501 gtaaatgtga aatctggaga atggaaggtg gttcaggtca tactgtaact gatgatatag
     4561 atcacttctt ttcagcacca tcaataacat atagagagcc acacttatct atatatgacg
     4621 tattggaagt acaaaaagaa gaacttgatt tgtcaaaaga tttaatggta ttacctaatg
     4681 ctccaaatag agtatttgct tgggaaacag gatggacacc aggtttaaga agcttagaaa
     4741 atgatggcac aaaactgtta gaccgtataa gagataacta tgaaggtgag ttttattgga
     4801 gatattttgc ttttatagct gatgctttaa taacaacatt aaaaccaaga tatgaagata
     4861 ctaatataag aataaattta gatagtaata ctagaagttt tatagttcca ataataacta
     4921 cagaatatat aagagaaaaa ttatcatatt ctttctatgg ttcaggagga acttatgcat
     4981 tgtctctttc tcaatataat atgggtataa atatagaatt aagtgaaagt gatgtttgga
     5041 ttatagatgt tgataatgtt gtgagagatg taactataga atctgataaa attaaaaaag
     5101 gtgatttaat agaaggtatt ttatctacac taagtattga agagaataaa attatcttaa
     5161 atagccatga gattaatttt tctggtgagg taaatggaag taatggattt gtttctttaa
     5221 cattttcaat tttagaagga ataaatgcaa ttatagaagt tgatttatta tctaaatcat
     5281 ataaattact tatttctggc gaattaaaaa tattgatgtt aaattcaaat catattcaac
     5341 agaaaataga ttatatagga ttcaatagcg aattacagaa aaatatacca tatagctttg
     5401 tagatagtga aggaaaagag aatggtttta ttaatggttc aacaaaagaa ggtttatttg
     5461 tatctgaatt acctgatgta gttcttataa gtaaggttta tatggatgat agtaagcctt
     5521 catttggata ttatagtaat aatttgaaag atgtcaaagt tataactaaa gataatgtta
     5581 atatattaac aggttattat cttaaggatg atataaaaat ctctctttct ttgactctac
     5641 aagatgaaaa aactataaag ttaaatagtg tgcatttaga tgaaagtgga gtagctgaga
     5701 ttttgaagtt catgaataga aaaggtagta caaatacttc agattcttta atgagctttt
     5761 tagaaagtat gaatataaaa agtattttcg ttaatttctt acaatctaat attaagttta
     5821 tattagatgc taattttata ataagtggta ctacttctat tggccaattt gagtttattt
     5881 gtgatgaaaa tgataatata caaccatatt tcattaagtt taatacacga gaaactaatt
     5941 atactttata tgtaggaaat agacaaaata tgatagtgga accaaattat gatttagatg
     6001 attctggaga tatatcttca actgttatca atttctctca aaagtatctt tatggaatag
     6061 acagttgtgt taataaagtt gtaatttcac caaatattta tacagatgaa ataaatataa
     6121 cgcctgtata tgaaacaaat aatacttatc cagaagttat tgtattagat acaaattata
     6181 taaatgaaaa aataaatgtt aatatcaatg atctatctat acgatatgta tggagtaatg
     6241 atggtaatga ttttattctt atgtcaacta gtgaagaaaa taaggtgtca caagttaaaa
     6301 taagattcgt taatgttttt aaagataaga ctttggcaaa taagctatct tttaacttta
     6361 gtgataaaca agatgtacct gtaagtgaaa taatcttatc atttacacct tcatattatg
     6421 aggatggatt gattggctat gatttgggtc tagtttcttt atataatgag aaattttata
     6481 ttaataactt tggaatgatg gtatctggat taatatatat taatgattca ttatattatt
     6541 ttaaaccacc agtaaataat ttgataactg gatttgtgac tgtaggcgat gataaatact
     6601 actttaatcc aattaatggt ggagctgctt caattggaga gacaataatt gatgacaaaa
     6661 attattattt caaccaaagt ggagtgttac aaacaggtgt atttagtaca gaagatggat
     6721 ttaaatattt tgccccagct aatacacttg atgaaaacct agaaggagaa gcaattgatt
     6781 ttactggaaa attaattatt gacgaaaata tttattattt tgatgataat tatagaggag
     6841 ctgtagaatg gaaagaatta gatggtgaaa tgcactattt tagcccagaa acaggtaaag
     6901 cttttaaagg tctaaatcaa ataggtgatt ataaatacta tttcaattct gatggagtta
     6961 tgcaaaaagg atttgttagt ataaatgata ataaacacta ttttgatgat tctggtgtta
     7021 tgaaagtagg ttacactgaa atagatggca agcatttcta ctttgctgaa aacggagaaa
     7081 tgcaaatagg agtatttaat acagaagatg gatttaaata ttttgctcat cataatgaag
     7141 atttaggaaa tgaagaaggt gaagaaatct catattctgg tatattaaat ttcaataata
     7201 aaatttacta ttttgatgat tcatttacag ctgtagttgg atggaaagat ttagaggatg
     7261 gttcaaagta ttattttgat gaagatacag cagaagcata tataggtttg tcattaataa
     7321 atgatggtca atattatttt aatgatgatg gaattatgca agttggattt gtcactataa
     7381 atgataaagt cttctacttc tctgactctg gaattataga atctggagta caaaacatag
     7441 atgacaatta tttctatata gatgataatg gtatagttca aattggtgta tttgatactt
     7501 cagatggata taaatatttt gcacctgcta atactgtaaa tgataatatt tacggacaag
     7561 cagttgaata tagtggttta gttagagttg gggaagatgt atattatttt ggagaaacat
     7621 atacaattga gactggatgg atatatgata tggaaaatga aagtgataaa tattatttca
     7681 atccagaaac taaaaaagca tgcaaaggta ttaatttaat tgatgatata aaatattatt
     7741 ttgatgagaa gggcataatg agaacgggtc ttatatcatt tgaaaataat aattattact
     7801 ttaatgagaa tggtgaaatg caatttggtt atataaatat agaagataag atgttctatt
     7861 ttggtgaaga tggtgtcatg cagattggag tatttaatac accagatgga tttaaatact
     7921 ttgcacatca aaatactttg gatgagaatt ttgagggaga atcaataaac tatactggtt
     7981 ggttagattt agatgaaaag agatattatt ttacagatga atatattgca gcaactggtt
     8041 cagttattat tgatggtgag gagtattatt ttgatcctga tacagctcaa ttagtgatta
     8101 gtgaatagat aaaaatatgt taaatatatc ctcttatact taaatatata aaaataaaca
     8161 aaatgataca ctacataaag tgttctatct aatatgaaga tttaccaata aaaaggtgga
     8221 ctatgatgaa tgcacagtag ttcacctttt tatatttcta atggtaacaa aatatttttt
     8281 tatataaacc taggaggcgt tatgaatatg acaatatctt ttttatcaga gcatatattt
     8341 ataaagttag taattttaac tatatcattt gatacattat taggatgttt aagtgcaata
     8401 aaaagtcgta aatttaattc tagttttgga atagatggag gaatcagaaa agtagcaatg
     8461 atagcatgta tatttttttt atcagtagtt gacattctta caaagtttaa ctttttattt
     8521 atgttaccac aagattgtat caatttttta agactaaaac atcttggaat atctgaattt
     8581 ttctctattt tatttatttt atatgaaagt gtaagtatat taaaaaatat gtgcttatgt
     8641 ggattaccag tacctaagag attaaaggaa aaaatagcaa ttttactaga tgcaatgaca
     8701 gatgaaatga atgctaagga tgaaaagtaa gtaatggtag atataataaa gatattaaca
     8761 aataaaaagt gttatccaaa taagaatagc tgaaagttat cataattcat gaaactaata
     8821 atgaaaacga gggagcagat gccaagagac acacaagtat taaatacata taatttcgaa
     8881 gcaagtgttc attactatat agatgacaag gtagtatatc aaacattggt tcacaaagat
     8941 ggtgcatggt cagttggtaa aatctattaa gctacattag ttacagatat cacaaactat
     9001 aatagttaaa catagaaata tgtgtaaatt gtgatggaaa ttattcaaaa acacaaaaat
     9061 acgtgatgaa ggacaaaatg atatagaaaa taagtatcaa accttaataa atgatttaat
     9121 tgatagttta aaagttatag gaaaaatata taaagaaata aaaacattaa aaaaatataa
     9181 gatatgttta caaattacta tcagacaatc tccttatcta atagaagagt caattaacta
     9241 attgagtatc tttaaattga aatgttagga agtgatttaa atatgaaaac ttaaattata
     9301 aaaaatcaat attaatttat ttttaaaaaa tagaaaggag tgtataagat ttattttcaa
     9361 agtttaaaaa caagaaaatc aatttaaatt tcagaaggaa taaatgtggt tatagaagtg
     9421 gatttattat caaaaataat aatactagga ggtttttatg tctttaatat ctaaagaaga
     9481 gttaataaaa ctcgcatata gcattagacc aagagaaaat gagtataaaa ctatactaac
     9541 taatttagac gaatataata agttaactac aaacaataat gaaaataaat atttacaatt
     9601 aaaaaaacta aatgaatcaa ttgatgtttt tatgaataaa tataaaactt caagcagaaa
     9661 tagagcactc tctaatctaa aaaaagatat attaaaagaa gtaattctta ttaaaaattc
     9721 caatacaagc cctgtagaaa aaaatttaca ttttgtatgg ataggtggag aagtcagtga
     9781 tattgctctt gaatacataa aacaatgggc tgatattaat gcagaatata atattaaact
     9841 gtggtatgat agtgaagcat tcttagtaaa tacactaaaa aaggctatag ttgaatcttc
     9901 taccactgaa gcattacagc tactagagga agagattcaa aatcctcaat ttgataatat
     9961 gaaattttac aaaaaaagga tggaatttat atatgataga caaaaaaggt ttataaatta
    10021 ttataaatct caaatcaata aacctacagt acctacaata gatgatatta taaagtctca
    10081 tctagtatct gaatataata gagatgaaac tgtattagaa tcatatagaa caaattcttt
    10141 gagaaaaata aatagtaatc atgggataga tatcagggct aatagtttgt ttacagaaca
    10201 agagttatta aatatttata gtcaggagtt gttaaatcgt gggaatttag ctgcagcatc
    10261 tgacatagta agattattag ccctaaaaaa ttttggcgga gtatatttag atgttgatat
    10321 gcttccaggt attcactctg atttatttaa aacaatatct agacctagct ctattggact
    10381 agaccgttgg gaaatgataa aattagaggc tattatgaag tataaaaaat atataaataa
    10441 ttatacatca gaaaactttg ataaacttga tcaacaatta aaagataatt ttaaactcat
    10501 tatagaaagt aaaagtgaaa aatctgagat attttctaaa ttagaaaatt taaatgtatc
    10561 tgatcttgaa attaaaatag ctttcgcttt aggcagtgtt ataaatcaag ccttgatatc
    10621 aaaacaaggt tcatatctta ctaacctagt aatagaacaa gtaaaaaata gatatcaatt
    10681 tttaaaccaa caccttaacc cagccataga gtctgacaat aacttcacag atactactaa
    10741 gatttttcat gattcactat ttaattcagc taccgcagaa aactctatgt ttttaacaaa
    10801 aatagcacca tacttacaag taggttttat gccagaagct cgctccacaa taagtttaag
    10861 tggtccagga gcttatgcat cagcttacta tgatttcata aatttacaag aaaatactat
    10921 agaaaaaact ttaaaagcat cagatttaat agaatttaaa ttcccagaaa ataatctatc
    10981 tcaattgaca gaacaagaaa taaatagtct atggagcttt gatcaagcaa gtgcaaaata
    11041 tcaatttgag aaatatgtaa gagattatac tggtggatct ctttctgaag acaatggggt
    11101 agactttaat aaaaatactg ccctcgacaa aaactattta ttaaataata aaattccatc
    11161 aaacaatgta gaagaagctg gaagtaaaaa ttatgttcat tatatcatac agttacaagg
    11221 agatgatata agttatgaag caacatgcaa tttattttct aaaaatccta aaaatagtat
    11281 tattatacaa cgaaatatga atgaaagtgc aaaaagttac tttttaagtg atgatggaga
    11341 atctatttta gaattaaata aatataggat acctgaaaga ttaaaaaata aggaaaaagt
    11401 aaaagtaacc tttattggac atggtaaaga tgaattcaac acaagcgaat ttgctagatt
    11461 aagtgtagat tcactttcca atgagataag ttcattttta gataccataa aattagatat
    11521 atcacctaaa aatgtagaag taaacttgct tggatgtaat atgtttagtt atgattttaa
    11581 tgttgaagaa acttatcctg gtaagttact attaagtatt atggacaaaa ttacttccac
    11641 tttacctgat gtaaataaag attctattac tataggagca aatcaatatg aagtaagaat
    11701 taatagtgag ggaagaaaag aacttctagc tcactcaggt aaatggataa ataaagagga
    11761 agctattatg agcgatttat ctagtaaaga atacattttt tttgattcca tagataataa
    11821 gctaaaagca aagtccaaga atattccagg tttagcgtca atatcagaag atataaaaac
    11881 attattactt gatgcaagtg ttagtcctga tacaaaattt attttaaata atcttaagct
    11941 taatattgaa tcttctattg gtgattacat ttattatgaa aaattagaac ctgttaaaaa
    12001 tataatccac aattctatag atgatttaat agatgagttc aatctacttg aaaatgtatc
    12061 tgatgaatta tatgaattaa aaaaattaaa taatctagat gagaagtatt taatatcttt
    12121 tgaagatatc tcaaaaaata attcaactta ttctgtaaga tttattaaca aaagtaatgg
    12181 tgaatcagtt tatgtagaga cagaaaaaga aattttttca aaatatagcg aacatattac
    12241 aaaagaaata agtactataa aggatagtat aattacagat gttaatggta atttattgga
    12301 taatatacag ttagatcata cttctcaagt taatacatta aacgcagcat tctttattca
    12361 atcattaata gattatagta gcaataaaga tgtactgaat gatttaagta cctcagttaa
    12421 ggttcaactt tatgctcaac tatttagtac aggtttaaat actatatatg actctatcca
    12481 attagtaaac ttaatatcaa atgcagtaaa tgatactata aatgtactac ctacaataac
    12541 agaggggata cctattgtat ctactatatt agacggaata aacttaggtg cagcaattaa
    12601 ggaattacta gacgaacatg acccattact aaaaaaagaa ctagaagcta aggtgggtgt
    12661 tttagcaata aatatgtcat tatctatagc tgcaacggta gcttcaattg ttggaatagg
    12721 tgctgaagtt actattttct tattacctat agctggtata tctgcaggaa taccttcatt
    12781 agttaataat gaattaatat tgcatgataa ggcaacttca gtggtaaact attttaatca
    12841 tttgtctgaa tctaaagaat atggccctct taaaacagaa gatgataaaa ttttagttcc
    12901 tattgatgat ttagtaatat cagaaataga ttttaataat aattcgataa aactaggaac
    12961 atgtaatata ttagcaatgg aggggggatc aggacacaca gtgactggta atatagatca
    13021 ctttttctca tctccatcta taagttctca tattccttca ttatcaattt attctgcaat
    13081 aggtataaaa acagaaaatc tagatttttc aaaaaaaata atgatgttac ctaatgctcc
    13141 ttcaagagtg ttttggtggg aaactggagc agttccaggt ttaagatcat tggaaaataa
    13201 tgggactaaa ttgcttgatt caataagaga tttataccca ggcaaatttt actggagatt
    13261 ctatgccttt ttcgattatg caataactac attaaaacca gtgtatgaag acactaatac
    13321 taaaattaaa ctagataaag atactagaaa ctttataatg ccaactataa ctactgacga
    13381 aattagaaac aaattatctt attcatttga gggagcagga ggaacttact ctttattatt
    13441 atcttcatat ccaatatcaa tgaatataaa tttatctaaa gatgatttat ggatatttaa
    13501 tattgataat gaagtaagag aaatatctat agaaaatggt actattaaaa aaggaaatt
//
LOCUS       DAEMHZ010000116        13527 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000116 DAEMHZ010000000
VERSION     DAEMHZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.116"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(141..518)
                     /locus_tag="KPK72_002932"
     CDS             complement(141..518)
                     /locus_tag="KPK72_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2490129.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            1423..1908
                     /locus_tag="KPK72_002933"
     CDS             1423..1908
                     /locus_tag="KPK72_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490130.1"
                     /translation="MSKSELTAETTEEMLKVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(2108..2647)
                     /locus_tag="KPK72_002934"
     CDS             complement(2108..2647)
                     /locus_tag="KPK72_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2490131.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(2640..3053)
                     /locus_tag="KPK72_002935"
     CDS             complement(2640..3053)
                     /locus_tag="KPK72_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2490132.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKNMEAVSYE"
     gene            complement(3089..4210)
                     /locus_tag="KPK72_002936"
     CDS             complement(3089..4210)
                     /locus_tag="KPK72_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2490133.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            4809..6461
                     /locus_tag="KPK72_002937"
     CDS             4809..6461
                     /locus_tag="KPK72_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG2490134.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            6760..7509
                     /locus_tag="KPK72_002938"
     CDS             6760..7509
                     /locus_tag="KPK72_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490135.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            7522..9549
                     /locus_tag="KPK72_002939"
     CDS             7522..9549
                     /locus_tag="KPK72_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2490136.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITTLLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDIK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            9693..11276
                     /locus_tag="KPK72_002940"
     CDS             9693..11276
                     /locus_tag="KPK72_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2490137.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESIS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWITLALEKDI
                     IKLNISDNGVANTSSKTPLDITKITFDTTKHKGIISIKEQVENLGGAINISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            11248..11868
                     /locus_tag="KPK72_002941"
     CDS             11248..11868
                     /locus_tag="KPK72_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490138.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLINSLLKVYQGVTCFPSENTEVIIEDLTSIEKKILQLICSGKKRK
                     MIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(12063..12989)
                     /locus_tag="KPK72_002942"
     CDS             complement(12063..12989)
                     /locus_tag="KPK72_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2490139.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4911 a         1919 c         1706 g         4991 t
ORIGIN      
        1 tatatgagaa aagctcaata gttaaatatt tagatagatt tttatataga cttttttaat
       61 ataaactttt ttaattgtag acttttttta ttagttttta atttttatta gttttgttat
      121 tggttttatt gttataatac ttataatact gccattgctt caatttctaa aactgcatct
      181 tttggtaatt gagcaacttg tacgcatgac cttgctggtg gattttcttt aaaatatgta
      241 gcatatactt cattaatatc attaaaatcc tttaaatctt ttacgaatac tgtagtctta
      301 aatattttat ccatacttgt accagcttgt tctaatattg cttttacatt atctaaagaa
      361 gcttttgttg cttcttttgg gtcagatact aactcgccag tcttcatatc tattggaagt
      421 tgacctgatg tagaaactat gtttccaact tgaattgctt gagaatatgg tccaattgct
      481 cctggagctt tctctgtagc tattactact tttgacatga cttcacctcc tattattaag
      541 ttatatttac aggaaaataa aatttcctaa acaccaatat taatacgaac ctttagatta
      601 aacttcaaat ctaaaacttc aaataatcaa tacttagatg attatgttta tttgtgtctt
      661 gttaactttt attaaagcaa ttaacgtgcc aaactttatt tatatataaa attttgtgaa
      721 gtcattcaaa atagttactt ctgaattttt tcgtaaaaaa acgctctaaa attacttttt
      781 tatgatgtca atttttttct catatctaga aatttaccaa catgagaatt cttgttttga
      841 gaatttacag attaaagtta ttttttaacc ttaaattctg aacctcccat gactaaagtc
      901 acagggttcc tgcttcatag aattttgcat aatcaagtta ttacatatga aagaactata
      961 gttgacaatc ccactattat aaataataaa acctttaccc ataccttatt taccctagtt
     1021 atcatagagc ctattgaaaa tataacagct aaatataata ataaccaaaa aattatcaat
     1081 atctaccact gttgctgaaa atccaaaaaa ctaccttttg atagtattac ttaacaatct
     1141 aattaatctc ataataaatt tacaacttat aaataaattg ttatattggc cgtatctcaa
     1201 caactttctc ctttcctagt tttaaatctt ttatagaatt gtaccacgtc taagtaaatt
     1261 ttcatactat taagtcttat ttctaataaa tcattagctc tcattctatg cccaataatg
     1321 taagattgca gtaattttat tgattttaat agttttattt aatatactta tattaattat
     1381 tttcatatga aattattatt tatatagggg gattttttta atatgagcaa aagtgaatta
     1441 acagcagaaa caacagaaga aatgttaaaa gtactaagtg gtaaagatta tgatattgca
     1501 tgtcatttac atgaacttgg taaatcatta gattgtaaaa ttgaaccaaa aacaggtgct
     1561 cgttcttaca aaatagtata ttcaactaag aaaccaaaac gtagcttatt tactattgaa
     1621 tgtaatgaaa aaaaatggag agttaaagca aatctttttc atctaaatac atataaagat
     1681 gctgtggagg aatgctctaa aactattaaa gatagtataa ctaaaactcg tacttgtaag
     1741 aaatgtaatt caaagtgtat tggaggttct tcgtttgaat tagatggaaa gtcttacctg
     1801 acttgcatag gaagtggtca ttattttgca aatatggaag aaatggattg gaaaaaccta
     1861 gaaaaattaa ttactaaaga aaataatatt atgcaggaat ctgtatagtt ttttaagggt
     1921 aattattaaa atatatgagt aacaacagat acttaaatag gaataaaaaa ataaagggta
     1981 taataaggat atagaataaa aataaatgat atagcacaaa gaggctgtct caaaatacag
     2041 ataagcctct ttttattgtt gaaatgataa gaatatattc aattttatac aattttatac
     2101 aaatatacta cgccaactta cccatttcat atgcctgttt cattgcctta cttcccttaa
     2161 tatcacctac attccaagca cctacaccat atactatacc tttttcctta gaaccattta
     2221 gacatgatgt aaatcctcta aatccttcta ttgttctttc catatcagcc acattttcat
     2281 ctgctgctgt aacgataaaa tagaaatctt tatcacaaat ttcagtatat ctagagcaag
     2341 ttctatcaat catagttttc atttgcgcat tcatagtata aaaataaact ggagtcgcta
     2401 aaacaatcac atctgcccca atcatttttt caagaatttc tcccatgtca tctgtctgtg
     2461 aacaatttgc ttctttttca taacaagcac cacatccagt acaatagtta atatctttat
     2521 cttttaaaaa tatcttctcc acttgatttt tagattctaa taccccttgt ataaactggt
     2581 cacacaataa atcagaattt ccaccttttc taggactagc tgatactatt aatacatttt
     2641 tactcataag atactgcctc catattctta tttctactgc tctttaatac tttttctttc
     2701 tcaataatac cttcttcata tctatcaatc ttaaaattta atcgttccaa agttttttgc
     2761 atttcttcta ttctttttgc tagttggttt ctctgctcta ttaaaagttc ttttctagct
     2821 ttgatagtac tgctcccttg ttgaaacata gctacatact caattaatgt ctcaatagaa
     2881 agacctgcac ctctcataca ctttatgaaa ttaacccact cacaatcttc ttctgtaaaa
     2941 gacctaattc cgcttttatt tctatttaca ggtggtatta aaccaattcg ttcataatat
     3001 cgtaaagtat ccgctgaaag ctcgtatttt ttactaactt ctgaaatcgt catacatatc
     3061 ttctcctctt gatattgtat agattgaatt atttctcaaa ggtttctgct acattcttta
     3121 aagcatctat aattttaatg tgttgaggac agctttcttc acattgttta cattcaatac
     3181 aatctgaggc ctttccataa gttttagtat aattattata gtagaccatc tgtgttgaaa
     3241 atccagtatt aagagcttgt ttttctgcat tgtacaacgc aaaatagttt ggaatagcga
     3301 tattttttgg acagccttct acacaatact gacaggcagt acaaggaact aaaattgatt
     3361 cgttgataat ctctactgct tcatctataa tattatattc ctcttgtacg aatggtttga
     3421 aattttgcat atatcctgta ttgtctaata attgttccat atttgacatc ccacttaaga
     3481 ccatcataac attatcttta cttgctgcaa aacgtattgc ccaagatgaa attgacatat
     3541 ctggattgta gtcattcaat aatttctctg ctttttctgg tatctttgca agtgtacctc
     3601 cttttactgg ctccataaca attacaggct tattatgctt ctctgccacc tcgtagcatt
     3661 ttctagattg tatgctttca ttatcccaat ctaaataatt aatctgtaat tgcacgaagt
     3721 ctacttctgg atgctcactc aagactttat ccaataattc tgcactatca tgaaaagaaa
     3781 aaccaatatt ctttattttt ccttcttttt tcttttcttc aataaactta aagctatcaa
     3841 attttttagc tacttcataa tgtgacacgc ctatattgtg taaaagatag tagtcaaaat
     3901 agtctacacc acatttttct aattgctcat taaaaatggt ttcttgctct tcttttttct
     3961 ttaaagccat taatggaagt tttgtagcta tagtaaaact ctctcttttg tgtcttttta
     4021 ctagagattc tctaagtgct atctcacttt ttcccatgtg atacacatag gccgtatcaa
     4081 aatatgtaaa tcccctttct agaaaagtgt ctaccatttt atttaaatgt tccatatcaa
     4141 tagttgtaac atcattttca tcttttattg gtaaacgcat taatccaaat cctaattttt
     4201 ttgcctccat tgttttactt ccccctttaa aacttgtttg ttttttctct ctactttaac
     4261 tattcttaca agtatataat tcttgtataa ttatacttta ctacttggag ttaacttcaa
     4321 gtcaatagat ttttttattt ttaagacaaa tattataaaa atacagttct ttaatattaa
     4381 tccattacat gtttctttca ccctccaatt ctcaaacttc acaaactcca acatgtaaag
     4441 aaatatatac cttaagaaat tcagtccatt aatattacta caaatacttt ttagatatgt
     4501 ttaaaaagaa atttcaccta aaatatttat aaaagattta gaaataggtc ttgtgtctat
     4561 tttaaaatat ataaaacagt ggtaaagact tatttataag ataaaaaatg aataatgtat
     4621 acccttttta aaaagttttt tcctttttat atttgtgata caatgttaaa tataaaaagg
     4681 aaaattttgt attaaagaaa tcttatttgt tgattttgta acttcaaact cagcatcaag
     4741 ttgattttaa gattatattt tattaagtat gctaaataac aaatttttaa agattgtgag
     4801 gtaaaaatat gaataagaaa aatttagcta tattaatatc aggtatgatg tttttttcta
     4861 gttcttcagc aatttttgca gacaatacta caaagcaaga aagattaata ggtaaaacaa
     4921 gatatgaaac agcagtagaa gtaagtaaat tgggatgggt tcaatcaaaa acagccatta
     4981 tagttaatgg taattctatt caaagtgcgc tttgtgcaaa tccatttgct aagctaaaga
     5041 atgcacctat acttttagta aacaacaaca gtattgaaaa ttcaactaag gctgagctta
     5101 aaaggcttgg tgtagataat gtgtacattg tagatagtgg aaatagcatt tcttctaaag
     5161 tagaaaatga aataaaatca cttaacataa aaataaataa aatagtagga aacaatatat
     5221 atgaaatgtc tacaaatgta cttaaagaaa tagataagat aaaaaagata gaaaatgttg
     5281 cagttgtaaa tggactaaag ggactatctg atgcagttag tatagcttct ccagcagcaa
     5341 tacataatat gcctataata ttagtatcag atgaaaacag caatgatagt agtataaatt
     5401 tcttaaacaa taggtctata aaaaaatctt acatagttgg aggaaataag attttatcag
     5461 acaatacagt aaagaaatat ccaaacagag aaagaatcag tggttcagat agaaacaata
     5521 ctaatgcaaa aattattgat aaattttata aagataaaaa aataaataat gtatttgtag
     5581 ctaaagatgg tattaaccaa gaaaatgcat tagtagattc attatcaata ggagtactag
     5641 ctgctaaaga aaattctcct ataatgatat ctaatggtag ccttgtaaat agtcaaaaag
     5701 aatttattaa aaataaagat atagatgaaa taacacaagt aggaggagga tataatcaaa
     5761 aagcatttga agatatttta aaactaaaaa atgtgaacat aagaaataaa actaaaaaaa
     5821 tatctttaat acaggctaaa gaaatattaa aacaagctct tccaggaagc aatattttag
     5881 tttgttatta tgatgaagat gataatgaat atgactgcaa agttataaag gataatatcc
     5941 tatatgagat agaaattgat gcctactctg gtaaaataat agaaattgaa aaagatgatg
     6001 acttaaatga taataattta ggtacacaaa atatatcatt ggataaagct aagtctatag
     6061 cattagatgc tgttcctaat ggtaatttaa tctattgtaa atatgatgct gaagataacg
     6121 aatacgaagc taaagtaata aaaaataaga aaacatatga attagagata agtgcatttg
     6181 atggaagaat tgtagatatt gacatggaca atgattctga tgatgacttt gatgataatg
     6241 ataagaataa ctctaatact aatgacaaca gttctattat tagtgctgaa aaagctaaat
     6301 caataatgct agataaagtg cctggtggga aaatagtaaa atttaaatat gatgaagatg
     6361 acaaagaata tgaaggtgaa ataataaaag gaaaaactga atatgagata acaattaatg
     6421 caattactgg taaaatatta gaatttgaaa tagatgatta ataaaaaata tatattctat
     6481 taattattac aagcaataaa gccataaata ttgaaatttt caatatttat ggctttattt
     6541 tatttttttt aatttaaatc cacattatgc cattatatat atctatgcac tcttttaaat
     6601 taaaatcttg tccatcatcc caaacctaat aaagttagat tttaaacaat atattgtgat
     6661 ataagtaatt tgtgtagaat tattgcgtga tttactcatg aagaaacaat aaggttaata
     6721 tataatttga ctatattaag acgaaaggag acatatacaa tgaatacaat attaaaagta
     6781 aataacataa ctaaaactta taataaaggg caacagccga gtttagataa ggtatccttt
     6841 aatgtatcag aaggtgaatt tataggtata atgggagctt ctggctctgg gaaaacaact
     6901 ctacttaatg tattatctac aattgataaa ccaacaagtg gagatatttt cataaataat
     6961 ataaatttga aatcacttaa tgacacttgt gctgctgatt ttcgtaagga taatttaggc
     7021 tttatctttc aagagtattt tttactagat agtcttacaa ttttcgagaa tatttcagta
     7081 cctcttacac ttcagaaact caacccagat aaaatagaaa acatggtaaa gagtctagct
     7141 gaacgatttg gcatagcttc tcaattaaat aaatacccaa atgaattatc tggtgggcaa
     7201 agacagagag cttcagcagt tagagcaata gtaaaacatc ctagtattct ctttgcagat
     7261 gaaccaacag gagctttgga ttctaattca gctactgaac tactccactc actacaagaa
     7321 gcaaatgagg agttgaaaac aacaatttta atggtaacac atgatgccta tgcagcaagt
     7381 tttgcaaata gaattttgat ttttaaagat ggtcatatca ttaaagaatt aaataaacaa
     7441 cagaataata gacatacatt ttatgagttg attattcaag aaattgcaaa attggatact
     7501 aaaagatagg aggataattt aatgaacatt ttttatatgg caataaagaa tcttaaaaaa
     7561 aacttttcat tctattctct atatctactt tcagtctctt ttgttattac tgtatttttc
     7621 gcatttactt cattttctgt taataagata atgctagaaa aaatttcaac tgatggtaga
     7681 gttgaatcta tgtgtaatgt tatttcgata tttttgatgg catttgtcat cttctatatg
     7741 gcatactcaa atcgattctt cttgagacgt cgtacaaaag aattaggtat atatgcttta
     7801 cttggctatc gtaagtttac aatattatcg ctacttacct ttgaaaatgt attaatttgt
     7861 agtggagctt ttattactgg attggtatta ggagcattgt cacataaggg tattgtcttt
     7921 ggaattacaa cactacttaa tttatcaatt gataattcac agataccatt ttttaatata
     7981 aatgcaataa acaaaacaat tatctttata gcattagttg ttttagtctt agctatatca
     8041 aatggtaaat ttctttataa gacttcactt gtaaaattga taagatatga aaaaagtgca
     8101 gaaaaaaaat taaagttcag aaaaattcca gcctttatgg ggtttattat gattttaagt
     8161 gggtattgtt tatcactaga tattcttcgt ggagctaaat cagtatgggt aagtattgga
     8221 ttttatcaaa tgggcttgat aactatgatt atcattgtaa ttggtacagt tctctttatc
     8281 aattcctttc ttccatatat tatgcaaaag agtaaaaaaa ataaacgtac tttctacaca
     8341 gatatcaaga taatagctat tccaaacttt atctatcgta tacgttcaaa ctctaaaact
     8401 ctaattatgc ttacattact ctctgctact actttgacag tttcaagtgt tatggcactg
     8461 tccttatact accctattgc atctgtttcg cgtattgcac cttcagagat agaatttaga
     8521 gtagaaaaaa ataaacaaat agaagatgta aaacaaattg tcagtcaata tacgtctaat
     8581 acagatgata ttatttttac acagacaaat atttataagg taaaatcttc atcaaaagac
     8641 cttccatttg aatatgaaat tggtacatct aagaataatt tatccaatag tagttcttct
     8701 aaagaagtta tttcaagaga atcaggattt gaatgtatat ctttttctga ttattatact
     8761 ttattaaagg cacaaaatcg aacaaagcta ctaaaacaac ttaccccttt aagggataat
     8821 gaatgtattt taatgaaata tcaaccaaat aatgatggat atagtgaaaa tggaacgatt
     8881 tatccattac ttgttaatgg taaagaaaca cctgtagctg taagggaaac atcattaaac
     8941 aatgctattt cttttgccaa tagcgttgga acacttatcg ttaatgatag tatatatgag
     9001 caaatagcac aaaataaatt accaatgaca agcattttaa gtattaatgg taaatcaatt
     9061 aaaaataatg aaaatttata tagtaatatt tcagattatt tagatggaag cccttattta
     9121 caaggatatt ctcacaggat aaatgattta ttatcaatta atagttctac atttctgtta
     9181 atcggatttc ttgtggtatt attctttatt gctgttggaa gtattttata ctttaacaat
     9241 atatcagcaa tttcagattc aacatctgat tataagattt tacagaaaat gggttatgat
     9301 aatatacaaa ttaaaaaaat tattaaaaaa caagtattgc cattttttaa tattccgttt
     9361 ttacttggta tattggactg tatatttgct accttggttt ataagactgc tttgatgcag
     9421 aatttacttg gaaattcatt ttcacaatac atacctgtga ttattgcaat tttcttaact
     9481 tctgtagtgt atttgatata ttatatactt actattaaat cctgttgtaa aattgtatta
     9541 aaaaattaat atttaccaaa tcgtcaagct tagaggtgtg acgatttggt atacttcatt
     9601 tataagtatt tgatataatt taaaattata gattcttagg tttgatttag tcataaaatt
     9661 aatataatta actcataagg aggtgtataa gattgacaaa ttttaattta aaaatactat
     9721 ttttaggaaa ctacattcta tcacttctct tcatgggaat atctacaagc actgaaactt
     9781 atatgaatat ccaaagcatt attcccttaa taatttcaac attattactc atagacatca
     9841 catatataac ctcttgtaat gtccgagaaa ataacattat ttcattgttt tgtggattac
     9901 tagcactaga cagttggtat ttactcttat catttgtaac cacaccaata ggcactatta
     9961 tttttaaaat attaagcaca gttatagtgt atgtctctat taaatttata tttttatttt
    10021 tgtttcaagg tagtggatac aatttccgta agataacaga cattttattg cttatcacct
    10081 gcattggtgc aattgtagga gcatttattt ctaacagaac gtatgcttgt atgtacggaa
    10141 tacagtttgt tgttagtata ataagctttt tctatattat tttttatcat ttgaaaaggg
    10201 ttgtttttgt cataaaaagc gaaaaaaaac ctatatcgat ttcagtattt ataacaatta
    10261 taggattcct tacttattat ttttttacac taagaatatc aaatcatatt ggtaattttg
    10321 gaatctatct aactgtactg gtatttttta tgagtattca tggtattgca cttaaagaaa
    10381 atgatagttt tcctatttca acagttttta acataaaaca aagggctata tttcttttta
    10441 taagtatttt agtaattagc ttggtcgtac tatttgccaa tgggaatttt tctataatat
    10501 taatagctat aaatgtttta tttatgctca ttttcctctg taacattatt ttggagttta
    10561 accttagaga tgaaaaaaat aaagtagtta aaaatagcaa gtatactttt gcattagaaa
    10621 aattacaaca agaagaacaa ctaaaaacag agttttctaa ttttttacat gatgacatat
    10681 tacagaacct gctttcaata aaaaacatga tgagtaagtc atatcgccca gaagttcaag
    10741 atattatata tgaaacactt aacaatctaa atacatgtat aaggaagcaa atgcaagact
    10801 atcaccctgt tattttaaaa aatttaacag taaaagaaaa ttatcagaat ttaattgaaa
    10861 gtatttcttc atcctttcca caaaaaaata tttgtgtttc atttaagtgt tctgatactt
    10921 tattcttagt tgaaccatat gatatattga tatatcgact gattaaagaa ttattgacaa
    10981 acgtatataa acattcaaat ggtagccatg catggataac tttagcacta gaaaaagata
    11041 taataaaatt aaatattagt gataatggtg tggcaaatac atcttctaaa acgcctttag
    11101 atataactaa aattactttt gatacaacaa aacacaaagg gattatctca attaaagaac
    11161 aagtagaaaa tctaggagga gcgataaata tttctgataa tatacctcat ggagtttgcg
    11221 tacagattac aatccctatg aaaggagatg gttcttataa atattttgtt agttgatgac
    11281 catatgcttt ttgctaaaag cttgtcaatt gcactagaag aatacaaaga aatcgaacag
    11341 ttttattcca cacaggacat ccataatctt tcagaaatga ttcaagataa aaaaatagac
    11401 attgtcttaa tggatataaa ccttagaggc ttaagtgaat gtgatggctt aaaagtagct
    11461 tataatttat tagagactaa ttcaactatt aaaataataa tcttgactgg atacgacctc
    11521 ccagtttata aacatgaggc taaaaaaata ggtatctatg gattcctaaa taagaatatt
    11581 agccctgata gtttaattaa ttcattatta aaagtctatc aaggggtaac ttgttttcca
    11641 tctgaaaata ctgaagtaat tattgaagat ttaactagta ttgagaaaaa gatattacaa
    11701 cttatctgta gtggtaaaaa aagaaaaatg attgctgatg aattgtatat aagtgaaaga
    11761 acattatcaa atcatttaca acacatatat gacaagctag atgtttcatc atcagttgag
    11821 gctattacaa aagctattaa aatgggttat atttctccag tatattaaaa aaagtgagtg
    11881 tctcaaaata aatcttctaa ctcataaagc actctccttt gtattaaatc aaatatattt
    11941 tcatcattta aacaattaag aagaggcttc tctctatttt gagacagctc cattttaata
    12001 cactatttat acataaacat attttaaaca actaaaaagc aactaatatc ttaataaata
    12061 tctcaataca tatcttcata acacatatcg ccataattac aagcagataa agaactctcc
    12121 tcatttaaat acgactcaaa ttcataccca aacagaattg ttggttctat atatatttcc
    12181 atatctgtat atctccatct tcttaccttt gcctctataa ccttgtaaaa atcttctaaa
    12241 ctataccctt cttttaatct tttatctata agtgccttag ttatactcaa atcagcttta
    12301 aaattttttc ctgattcact atttaatttt cttataataa tttcatgtat gctttctgga
    12361 tttttataag tagaatcttc agtcaaatct tcatccaaat tttcaaaggt atttttattt
    12421 aaattctttt tatttaaatt attttttaat aattcttttt tctttgtctc attttctgtt
    12481 tcatacccag tatgaattat caaatcataa tctttgtcta tactctcttg atttatacgg
    12541 ctgtatgtgt tggtattact gttcttaaac aactcatttc tatgtttata tttcattgtt
    12601 ttttggttat tgttaatagt tgcatcaatg tatttctcta tatttttgaa aatatcacca
    12661 ttctttgtta caatttctgc attactactt tcagaacaaa cataactata tacagaacaa
    12721 caatttccct ttactcccct acttattaat ctcaatatac ctaaatctat aaattcattt
    12781 acaagtctac ttattgtaga tatagataca tcaaggtcag atgcagcttt ccttactgtc
    12841 atcataaatt gtcctcttgg cagagttcct tttaatcttt gtatattttc ttttctcatt
    12901 acatagtgat taaaccttat tctatttaca tctctttttt cattaaaata taatactgct
    12961 gaatttaatt gataaaaact attactcata ttcccactcc tccaatttaa atagataatt
    13021 tgttgtaaag tttttagatt tttaaataac ttattaaatg attattttta ttaaaaagta
    13081 ttctttgata ttttcatttt aaagttttaa gtataataag ttaatagtgt agatttaact
    13141 tttttattta aacaataaac ttaatattat ttttaatata ataattttat taaatttagt
    13201 atgtaatttt ttaatcttat tttattccct ctatactctt atgctaaata ataactttga
    13261 taatatttcc ctaacttatg tttcaactgg tttatattgc aatttattta attacagctt
    13321 atttgcataa taattcgtcc atatcataag ttgtgtattt atataaatac acaattaaaa
    13381 ataaatcact gtggtatact aattattacg aagactatac ttatatgcaa agacaatata
    13441 aggcatctta tatcttttat taattataaa tatcttttat caattataaa aaattaaacg
    13501 ctatcttatt tatcataaat tacaaaa
//
LOCUS       DAEMHZ010000117        13174 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000117 DAEMHZ010000000
VERSION     DAEMHZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.117"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            149..2008
                     /locus_tag="KPK72_002943"
     CDS             149..2008
                     /locus_tag="KPK72_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2490140.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2282..2881)
                     /locus_tag="KPK72_002944"
     CDS             complement(2282..2881)
                     /locus_tag="KPK72_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2490141.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2984..3781)
                     /locus_tag="KPK72_002945"
     CDS             complement(2984..3781)
                     /locus_tag="KPK72_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2490142.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3894..3999)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4091..5077)
                     /locus_tag="KPK72_002946"
     CDS             complement(4091..5077)
                     /locus_tag="KPK72_002946"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2490143.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5561..7291
                     /locus_tag="KPK72_002947"
     CDS             5561..7291
                     /locus_tag="KPK72_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG2490144.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7401..7481
                     /locus_tag="KPK72_002948"
     tRNA            7401..7481
                     /locus_tag="KPK72_002948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7435..7437,aa:Leu,seq:tag)
     gene            7816..8331
                     /locus_tag="KPK72_002949"
     CDS             7816..8331
                     /locus_tag="KPK72_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG2490145.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKI
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8823..8927
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9137..11611
                     /gene="gcvPA"
                     /locus_tag="KPK72_002950"
     CDS             9137..11611
                     /gene="gcvPA"
                     /locus_tag="KPK72_002950"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG2490146.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKEAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11611..13068
                     /gene="gcvPB"
                     /locus_tag="KPK72_002951"
     CDS             11611..13068
                     /gene="gcvPB"
                     /locus_tag="KPK72_002951"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG2490147.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNILVKRVDDARAVKKPILTWSQR"
BASE COUNT         4940 a         1573 c         2180 g         4481 t
ORIGIN      
        1 gaggactcta aagatgaact atgtaaaaca aggtttatat attctaattg tatagaaaat
       61 ttattgtaaa cgcatgtata ttattagaaa aaatagtttg aaggtattca ataaacataa
      121 aaaacaatat aggggataaa ggtgttttat gaagagattt ttgaggagaa ttatcttagt
      181 tttatttatt ttgttgattt ttataagttt tatttctatt aaattaattc ataatgttgg
      241 agattatgga aaattgatta attatgttgg gattgttcgt ggagctagcc aacgtctaac
      301 taaactagag atgaatcatc aaccaaatga tgaattaata gaatatattg atgaaatcct
      361 acaggagttg attacagggc atggagatta tggtttagta atcactgatt gtaatgaata
      421 caatgaggac ttacttttat tagaaaaaaa gtgggaagat ttaaatattg aaattaaaaa
      481 agtaagaatg aaagaacaga ataaccagtt attaagtata agtgaagagt ttttttctct
      541 tgcaaatgat acagtgttta aaatagaaaa cttttctaaa gaaaaaagta attacttaat
      601 gactttaata ataattatat ctattatagg tatattagca tgtatcatat tgattttaca
      661 atatagtaaa aaaatggtta gactggaaaa attaaatgta gatttaaaaa atattgcata
      721 taaagatgaa ctaacaggag taaatactat agaaaaattt aaattggatg caaatcaaaa
      781 tatttgtata cataaggata agaaatttgc tgtattctat attgattttg aaaactttaa
      841 atacattaat gatatatttg gatatgatta tggtgacatg atactgaaaa gatatgctaa
      901 tcttatgatg aatgacatag ggaaatatga aatatttgca agagaaattg cagatagatt
      961 tgttgcgtta agatgctatg tagataagga agatttagtg gtaagacaac gagatgtaga
     1021 caatgaacta attaatataa caaatgaaat taaaaataaa catagtatta ctgttgttag
     1081 tggaatttgt tgtattgaag atgtaaatga aaaattaagt atagatggtt taataaatag
     1141 agcaaatttt gcacaaaaaa ctgttaagaa taaaccaggt actaattatg ctttttataa
     1201 tgacagtatt cgtaaaaaaa tgattgaaga aaatactatc aagagtagaa tacatgaagc
     1261 tattgaaaaa agagaattta tagtttattt acagcctaaa gttaatttac atagtcaaaa
     1321 aataaattgt gcagaagctt tagttcgttg gcttactccg gataaaggta ttatatcgcc
     1381 agcaatattt attccagtac ttgagaagaa tttttttatt gccttagtag ataagtatgt
     1441 ttttgaggag gtttgtaaat ggattagaaa gaggttagat gaaaataaac cttttgttca
     1501 gatatcagtt aatgtttcaa gaatacaatt ttataacact aaatttgtag aaacttattc
     1561 caatatacaa aataaataca ggatacctaa aaatactata gaaattgaat ttacagagag
     1621 tgttgctttt gagaatcaaa aacatctttt ggaaattata catgatttgc atgaaaatgg
     1681 atttacttgt tctttagatg attttggaaa aggttattcg tctttaagtg ttttaaaaga
     1741 tttaccattt gatgttttaa agcttgattc tatgtttttc aaggctagtt tagataaaga
     1801 taaagagaaa atagtaataa aaaatattgt acatatgtta aaagaactta atatcacaac
     1861 tgttgctgag ggcattgaat gcaaggaaca agttgaattt cttagggata ttgggtgtga
     1921 tttagttcag ggatttgtat tttataaacc aatgccaata ttagaatttg aaaaaatatt
     1981 ggataaagag tttgtttata atagctaaaa tgtactttat aaaatatgaa ctaattaagt
     2041 aaaattattt tgtactgaat aaatttttac tttatttaca gaaaagcgcg aaaagtgtat
     2101 tcctttaagt gtgggatgga tagcacaaat agaggagaaa aaatattggc catataagaa
     2161 tgcataataa tataaatatt tgaaaggtca gcattatata aaaattaaaa agaaaataaa
     2221 taatttttct ataaaaaaga gactatgaga aaatttactc atagtctcct aaataatgta
     2281 tttattttct tcctgcaacc cattttaatc cccaatgaaa ttcagtatcc atttctctat
     2341 gacctttgaa gtatttaact actttttcaa cactgaatgt ctcatcattt acattttcta
     2401 tcaatgctat tgaacacata ttatagccat ttttatgttc atcaagtttg acaactaagt
     2461 ctgaatcttg tttttgtttg attgttacaa ttccatcagc ttcagaccag ttagctacac
     2521 cttcgtatat aaaagcataa ataagtattc tttttatatc ttttatttta ttaccattta
     2581 ttcttaaatt ttctccttgt gtattgcttc ctgttctgtc atctccatct aattgagcaa
     2641 aaggtggatt gtctaaacta ccaaaggcat ttcctaatgc ttgaacagca ccttttacac
     2701 catttttcat ctcaaatagg caacccaaat ctaaatctat attactgctt cttaaagagc
     2761 caaaaaaacc ctttttttgt actttttgat tccaatttaa attaactaag atttcaccta
     2821 aatcgctatt ttcctttttt gtaaggttta ttttttgtcc cttttttaat tctatagcca
     2881 tagtaatctt cctccacaaa catagattta tttaaatggt agcacttaat aagtcaatat
     2941 tcaacaagat tagaataata atattaaaat tatactaaat atactaaaaa gtaggaatca
     3001 caataccttt gtatttttct tcaattaagc ttcttgcttt atctgatgta agaacatctt
     3061 gtagaacttt aatcttttca ctgtctttat cttcagttct aaatgctaaa agatttgcat
     3121 actcgtcatt tataggagct gtataaattc cgtttttctt ggcatcaaga ccagcaggta
     3181 cagcataagt agtatctata aatgcagcag tcacatctgg aagagaagat ggaatttgag
     3241 caatatctaa ctctttgaat tgtattccct ttggattttc tattatgtcc ttaggactaa
     3301 ctaatccatc ccctttaggt aacgttaaaa gtccaatact ttcaaggtat ctaaggttct
     3361 tagaacaaga agatgggtta ttgcttattg caattgtatc tcctttttta aattcatcaa
     3421 cagattttat cttataagag taaagtattg ctgggcatac atatagtttt ttaccagcca
     3481 ttattttgta acctttggcc ttaacagctt cttttagata aggttcatgt tgaaatagat
     3541 ttgcatcaat ttcaccatta tttaaagctt cgtttggaag tatgtaatca tcaaagtttt
     3601 taacttctaa agtatatcct ttttctttaa taagtggttt taattcttct aataactctc
     3661 ctcctggaac taaagtagca ccaacaacaa tttttttatc atctttagct ttagaacatc
     3721 caaccacagc taaagttaat actagacata ataacaaaca taataatttt ttgaatttca
     3781 tataaattcc ctcctgaata taattgatta gttaaaaata aaataccatt ctttatcaca
     3841 ctgcaaaata aaaataagcc tgagtttaaa ctcaggcttt aaaagctaat ttagactcat
     3901 ctttcagtat aatactgcag gatttagcac caatgtatat aaaaatatac tggttgctgg
     3961 gtttcatagg gccagtccct tcaccactct ggataagaag tatcaatttt taattatgtt
     4021 ataaatatta tcatgatttt ttacgtctgt aaacataatt ttataaatat tttaaaatct
     4081 ttaatgtatt ttatttaaca cctaaaattc cagaaactag aatatcagta tcagcaccta
     4141 agaaatagtc atttatttca atagaatcca gagtatttct caaagcattt tcttcatgcc
     4201 ttataccaat aagtttattt tctacaaatg caacatcaca ttttccaaaa aaatctccaa
     4261 aaaattttat actagttatt atgcctttat caacatttaa attaaactca acatttccac
     4321 cagaatattt taattcattt gaaagagtat attttggtga attaccaaaa ttccaatccc
     4381 aagtttcgta ttttgattta gtaagctttt ctatttgttt tatatcatgc tcactcaaac
     4441 aataattagt attatcagta tttgttttag aaagataatc cattatagca tctttaaatt
     4501 ctaatatgtc catttttgaa tgtaaatgtt cacttatatt ggttacacga gattttacag
     4561 attttattcc ttttccttca aattttatag gtttaacctt taatgctttt gagaggtcat
     4621 ttatttcaga tgaaaataaa agagtcccat gatgcattac tttgtttttg tagttgtatt
     4681 gagcatttcc agaaaatttc ttgccatcta taagaaggtc atttcttcca gaaaattcgg
     4741 cattgacatc tagtgtttta agtaaatcta ttatgggctg agtgaatctt ctgaaatcac
     4801 taaaattatt attgttacaa gctataaatg taaaatttat atttcccaaa tcatgaaata
     4861 cagctccacc accagactgt ctcctaacga caggtataga atattctttt atatattcta
     4921 aatttatttc agaaagtgtg ttttggtttt ttccaacaat tatagaaggt tcattcctcc
     4981 ataaaataaa taagtcttca tcaaaatttt ttagaagata ttcttccatt gctaaattaa
     5041 aataagggtt tgttttttca ttataaatta ataacattaa agtagttcct ccatagaatt
     5101 gataaaattt ttattatgaa tacaattaat tttataattc gacaataata taaaaactcc
     5161 tttaaattag aaattttaaa aactaccttg caaagtttaa ataaataata tatactcaaa
     5221 atatgtttaa taaaagtgaa tagaagggta gaggcgcaat aatctatcag tactaataag
     5281 gagttaagca cagagagtta ttaggaaagg agatattgcc gaagttttat aatcgatgct
     5341 ttaagattat attgctgggg gtacatagaa aatatgtaca actgtcacag gtttatttgt
     5401 ggtgagctat catttaagga attatattat atttattttt actattgttg atatattgta
     5461 atgtttaagc cttgagtgtt taccactcaa ggctttttta gttttattgt aacataatac
     5521 cctgtacagt tcacaaaatt aaaaggagag ggagatttat atgaggaaca aaaaaattaa
     5581 tattattttt ctgacgacaa tcatgttcat aatgtcaaca gtaatggttt ttgcagaaga
     5641 agatatagac actattgcac tagccaatgc agaaaaattt gggattttaa ctttaatacc
     5701 accattagtt gctataatac tcgcatttat aactaaaaac gtaataattt ctttacttat
     5761 aggtattctt tctggtagtt ttataattaa agctagtggt attaatgttt ttgcgacatt
     5821 tatacaggca tttttagatt tagtagacag agctttagta tctttagcag acccttggaa
     5881 tgcggggata atactacagg ttttggcaat tggaggagta ataaatcttg ttgctaaaat
     5941 gggaggagct aaagctatag ctgaagcact tgctaaaaga gcaaagtcag caaaagggac
     6001 acagcttata acatggtttt tggggctttt agtattcttt gatgattatg ccaattcact
     6061 aatagtaggt cctatgatga gacctgttgc agataaaatg aaaatatcga gagaaaagct
     6121 agcatttata atagatgcca cagcagcacc agttgctggt ttagctataa tatctacatg
     6181 gataggtctt gaggttggtt taatacatga tgcttttgaa agtataagta tagatgtaga
     6241 tgcatttggt atatttttaa atacaatacc atttagattt tacaatatat taatattagc
     6301 tttcattgta atatcagcat tattattaaa agaatttggt ccaatgagaa aagctgagat
     6361 aaaatctaga agtagaaaaa ttagtataga ccttgatgaa ggcgtagaag agttggatga
     6421 tttagctcca aaaaatggag taaaattgag tgtatggaat gctataatcc caataggaac
     6481 attgataata gttgcattag catcatttta ttatagtgga tatacatcta taatgggagg
     6541 agacgataag gctctgatac aactatttac aaattctcca tattcttttg aagcaataaa
     6601 agaagcattt agtgcatctg atgcatctag agcattattc cagtctgcac ttgttgcaag
     6661 tttagttgct attataatgg ctgtagtaaa aaagattttt acaatatcag aagcaattga
     6721 tgtttggata gatggaatga aaagtttagt aatcactggt gtaatattaa tacttgcatg
     6781 gtcattaagt tcagtaataa aagagttagg tacagctaaa tttttaatac acctattatc
     6841 aggttcacta ccaccattct tattaccaag tttaatattt ggtttaggag caataatatc
     6901 ttttgctact ggaacagctt atggaacaat gggtatactt atgccacttg caattccatt
     6961 agcatactca ttaaatcctg atatgtctta tgtaatagta agtactagtg cagttttaac
     7021 aggtgcaata tttggagacc attgttctcc aatttcagat acaacaatac tttcttcaat
     7081 gggagctggc tgtaatcata tagaccatgt taacactcaa atgccatatg ctatatttac
     7141 agcagtaatc actattgtat ttgggtatat accagctgga cttggattgc caatatatat
     7201 agtattacca gtggcaatag ctgctatatt tgtaggtatt caaataattg gtaagaaagt
     7261 tgatgaagcg gaaatagaac ttgtagaata aagatataaa taagtttcac cttttattat
     7321 cactattttg ttagttttta gataatttta aaaagttatt gaaaataatg gcgaaatata
     7381 gtaaaataaa agagtgataa gcggatgtgg cggaattggc agacgcacta gacttaggat
     7441 ctagcgccat tggcgtgggg gttcgactcc cttcatccgc actttacagt acttaattgt
     7501 actgaaaaaa actggggttg ctggctttcg gagtgggcta tcccgttttt atttttttgt
     7561 atatataata gattggcact ttagtataag gtgctttttt tataggcaaa aacagtacat
     7621 ttatttctgt tttattatca ttcatctatt agaatatact ttagttaaga atgtgttaat
     7681 aagcagtgtt cacattcttg ataaaatgtg gaataatatg tatttttact aagacggaaa
     7741 tgaaataatt aaaccattaa ataatattat aagatgatga ataagtagat aaatcaaatt
     7801 ttgtaggagg atattatgga aaaatatata tggttgtttc cattactttt tatttttcat
     7861 gatatggaag agataatagg ttttggaata tggcttaaaa aaaataaatc aatgcttgat
     7921 aagaagtatc catttatcag taaaatttat gaaaattata gtactgaagg catggcattt
     7981 gctgtgtttg aagaatttat tttatgcatt attttctgta tacttacagt tataacagaa
     8041 aatcagtatg tatatctact atggttaggt agttttatag catatacctt acatttggta
     8101 attcatatag ggcagtcaat cataataaga aaatatatac cttcacttat taccagtatc
     8161 atatgcttac caataagcat ttggtgtata agtaagagta tttatatagt tgattgtgaa
     8221 atgagtacaa caattctgta cagtataatt ggcattataa ttgtagcatt gaatttaaag
     8281 tttgcacagt cgctgattgg aaaatttaca aagtggatgt caaacatata aattttagtt
     8341 tattggacag ttcaaggaat cagaaattgg aattttatag tattaatatt ttatagtatt
     8401 aatataatgt ataaaatttt atagcaatgt atttcataag tataaagtga ctagaatttt
     8461 aagaaatata caaattctat aaaaactaaa taatttcaag tatattttaa aaatcttatt
     8521 tttcacaagt ttaaaattgt aaacgatttc atcaagtcta tgaatatact tttaacatgt
     8581 tatagtaaat ctcaatataa atttacattt ggttctttgt gtgttatttt aataataaat
     8641 taagtgaaaa tgttatttta cattaatata taatatcttt gcataagagt gaataacata
     8701 aaagagaaat aattgataat aaaaacttaa caaaaaaatt aatacataaa aaaaaataaa
     8761 tataatgtgt tgacatttaa ttgaaatatt catacaataa aattgaaaat aaatatttcc
     8821 aaatgagatt agcgagagag tcctttatac gaaaaggcgc cgaagaagaa atttaagaaa
     8881 tgccaatttt tagagaagct ttcaggcaaa aatatcgcta attgatggga ctctggagag
     8941 acttaagtta agatactata agtcaccgaa gaagcaaaat aatattaaat tattaaatct
     9001 ttcaggtata caaacaggga tatataggct aggattattt tccatgctga tatatctttt
     9061 ttttatgaaa aagtacagga aataatatca aagttactta aatacagtag gtctaatttt
     9121 ctagggaggg ttatatatga atgaattgaa aagagtatct ttgtacaata tacacaaaga
     9181 gcttggagca aaacttgttg aatttgctgg ttgggaaatg cctttagaat atgagggtat
     9241 aaataaagag catgagaagg tcagaaaaag tgcaggtata tttgatgtat ctcatatggg
     9301 agaggtacag ataaaggaag cagagtcaga aaaatttata caaaacctag ttacaaatga
     9361 tataagcaca ttaaagataa atgacattat atatacacct atgtgttatg aaaatggtgg
     9421 agtagttgat gatttattaa tatataaatt tggtgaagaa gattatttat tagtaataaa
     9481 tgcaggtaat atagataaag atgtagcatg gataataaaa caaagtgaag gatacaatgt
     9541 agatataaaa aatatatcta gtgaagtgag tcaattagca attcaaggac ctaaagcaga
     9601 agaaattctt caaaaaatca cagatataga cttaaactca ataaaatttt ataagtcaat
     9661 tccatcaaca aaagtatgtg gttgtccttg tttagtttca agaacaggat atactgggga
     9721 agatgggttt gagatatact gtaaaaataa atatgtagaa ataatttgga atgaagttct
     9781 aaaggttgga ggagaagata tatgtccagc tggtttaggt tgtagggata ctttgagatt
     9841 tgaagcagct ctacctctat atggacatga aataaatgaa cacatatctc caattgaagg
     9901 tggtttatct atctttgtga aaacaaataa agaatcattt attggcaaat caattttatc
     9961 aaaagaaaaa gaaagtggag ctaaaagaaa gttagtagga tttgaaatgc aaggtaaagg
    10021 aatgcctaga aatggatatg atataaggat tggtgataaa acagttggat ttgtaacaac
    10081 aggatgtgca tctccaacga caggaaaaat tttaggtatg ggaattatag attctgaata
    10141 tgcaaaagta ggaaatgaaa taggtatagc aatcagaaaa aaagttgttc ctgctgtgat
    10201 agttaagaaa cctttttata aaaaacaata taaaaaagat aatattatat taaacaaaga
    10261 aaataaattt tcatatatac ctgctacaag tgaagataaa tcaaaaatgt taaaagtagt
    10321 tggattaaat tcggttgatg aattattttc agatatacca gaagaagtaa aattaaaaag
    10381 agatttaaat ttggaaattg gaaaatctga attagaggta agcaaaatag taaaaagatt
    10441 gtcagaagaa aatttaagtt tagaagactt gacttgtttt cttggtgctg gtgcatatga
    10501 ccattatata ccatcaatta taaaacatat aacttcaaga tctgaatttt atacagcata
    10561 tactccatat caagctgaaa taagtcaggg aactttacaa gtagtttttg aatttcaatc
    10621 tatgattgca gaaattacag gtatggaaat tgcaaatgca tcaatgtatg atggagcaac
    10681 tgctgcaata gaagcatgta taatggcaat gaatcagact agaaaaagta aaatagtagt
    10741 gtcaaaaact attcatcctg aaactttgtc agtattaaga acttacttac aatataaaga
    10801 ttgtgagata gtagaaattg acttttgtaa tgaatatggg actacagata tagaaaaatt
    10861 aaaagcttct gtagacaaag atacagcatg tgttcttata caaactccta atttctttgg
    10921 aattattgaa gaaatggaag aaatagaaaa gataactcat gaaaataagg ctatgttaat
    10981 tatgagtgta gatccaatat cattaggagt tttaaaaaca ccaggtgaga taggtgcaga
    11041 tattgttgtt ggagaagctc aatcacttgg aaaccctctt aattttggag gtccttatgt
    11101 aggattttta gctagcaaat ctaaatatac tagaaaaatg ccaggaagaa tagttgggca
    11161 aagtttagat gtagaaggta aaattgcata tgtattaact ttacaaacta gagaacagca
    11221 tgtaagaaga gaaaaagcaa cttctaatat atgttcaaat caagctttaa atgctcttgt
    11281 agcttcaata tatatggcta ctatgggtaa agaaggattt aaagaggttg gtatgcagtc
    11341 tatgaaaaaa gcacattata cctacaataa acttgtacaa actggaaaat ataagccaat
    11401 atttaaaggt aagtttttca aagaatttgc tgtacaagga aatcttaata tagaaactat
    11461 aaatgataaa ctcttagaag aaaatatatt aggaggttat aatttagaat acaattatcc
    11521 agaattaaaa aattcaactt tgctttgtgt gactgagaaa agaagcaaag aggaaataga
    11581 taagttagtt ggaataatgg agggattata atgaaagaat acaatagttt gttgatagat
    11641 atatctaaaa aaggtaggaa agcatactca cttccaaagt tagatataga tgatataaaa
    11701 atagaagata tgattgaccc agatatggca agacaaagtg agttgaattt gccagaagtt
    11761 ggtgaattag aattagttag acattataca ttattatcaa ataaaaactt tggagttgat
    11821 acaggttttt atcctttggg ttcttgtact atgaaatata atcctaaaat aaatgaagat
    11881 atggcagcac ttccaaactt tactggaatg catccatacc aatcttcgga tacagctcaa
    11941 ggttctcttt ctttgatgta tgatttatca agaagacttg cagaaattac aggtatggat
    12001 gaagttacac tacaaccttc agcaggttct catggtgagt ttacagggct tatgattata
    12061 aaagcatatc atgaaaatag aggtgaccat aagagaacca aagtgataat tccagattct
    12121 gcacatggaa caaatccagc aagtgcagct atggcaaact ttgatgtgat tcaaatagca
    12181 tctgataaaa atggagctgt agacataaat gcattaaaag aggttttgaa tgatgaagta
    12241 gcagctctta tgcttactaa tccaagtact cttggtttat ttgaaaaaaa tataaaagaa
    12301 atagctactc ttgtacatga agcaggagga cttttgtact atgatggtgc aaacatgaac
    12361 gctattatgg ggataacaag acctggagat atggggtttg atgtggttca tcttaatctt
    12421 cataagactt tttcaactcc tcatggaggt ggaggtccag gagctggtcc tgttggagtt
    12481 aaaaaagaat tagtaccttt tttaccaata ccagtaattg aaagaaaaga agataagtat
    12541 gtactaaatt atgatagaga aaaatctatt gggaaaatta aaaatttcta tggtaatttt
    12601 ggagttcttg taagagctta cacatatata ctaactatgg gaagggatgg acttaaagag
    12661 gctagtgaaa tggcagtttt aaatgctaat tatataaagg aaagtataaa agatgattat
    12721 attttgccaa tagatacctt gtgtaagcat gagtttgtat taggtggatt gccaagagga
    12781 gaggccaata taaaaactat cgatattgca aaaagactac tagattatgg ataccatcct
    12841 ccaacaatgt actttcctct tattataaat gaggcactta tgatagaacc tacagaaagt
    12901 gagagtatag agacattaga tagttttata gaagctatga aaagtgttgc taaagaggca
    12961 aaggaagaac cagaactatt gaaaactgct ccacataata ttttggtcaa aagagtagat
    13021 gatgcaaggg cagtaaagaa acctatacta acatggtcac aaagataaat agttaaaaag
    13081 tagagattta aagtataaag agaattaaat ttataagaaa aagctatcta acttaatcta
    13141 gtttagatag cttttttgta tattagtata tatg
//
LOCUS       DAEMHZ010000118        13145 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000118 DAEMHZ010000000
VERSION     DAEMHZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.118"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(456..1283)
                     /locus_tag="KPK72_002952"
     CDS             complement(456..1283)
                     /locus_tag="KPK72_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2490148.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            1889..2344
                     /gene="smpB"
                     /locus_tag="KPK72_002953"
     CDS             1889..2344
                     /gene="smpB"
                     /locus_tag="KPK72_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG2490149.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            2502..2851
                     /gene="ssrA"
                     /locus_tag="KPK72_002954"
     tmRNA           2502..2851
                     /gene="ssrA"
                     /locus_tag="KPK72_002954"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2967..4994
                     /locus_tag="KPK72_002955"
     CDS             2967..4994
                     /locus_tag="KPK72_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG2490150.1"
                     /translation="MSIDNNFNHIAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIVKSVYKEYDFLPEEEKDELVIVDLKENHNIDLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNSVKEIIGKFKFKDEIAQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNEMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEMLFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKVNPSAIVNVYGQELLDESFAICQADMIMKGQNPDNIRLGNT
                     LTQDRFSGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIEKDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEDKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEEIKEI
                     YERFEESENSKIFDNEGFGYRKVTIERPLKLSFRVNEEAIENVKNTTQFINLSVSKKK
                     DEEVKVKEEAEGRVKQDKLLKLLESFDSEFEYMKRDKFIKDLKSKSKLYDVALSAGLI
                     KAIVNAIGVRNEDAVVCKDAKGNIESDSSLKDTESIALKEDVYEYFEKEVKPHVEDAY
                     IDESSIDNIGYEIPFTRYFYKYEKLKSFDDIMKEVESLESEIALEIRKVLG"
     gene            4994..6361
                     /locus_tag="KPK72_002956"
     CDS             4994..6361
                     /locus_tag="KPK72_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG2490151.1"
                     /translation="MRYRYRDDEEMKDSGVEWIGKIPKDWEVKRIKHLFELKKDKSDE
                     ENPTVLSLTQKGLKIRDVSNNEGQLASTYVGYTKIEKNDFILNPMDLISGYTDKAEIE
                     GVISPAYTTFRSKNKVNINHDYYKRYFQMHYHHNFLFPWGEGVSFEHRWTLKNEVFLN
                     LPVITNRLEEQEKIANFLDEKTSQFEFIISKKEELIKKLEEAKKSLISEVVTGKVKVV
                     KTDDGYKLVKRSSEEMKDSGIEWLGEIPKDWEVKNFKYMFTLNKGLSITKADLKESGI
                     PCVNYGEIHSKYRFELKPSKHKLKYVDESYLKSNSISLLKYGDFVFCDTSEDIEGCGN
                     FTYLNENNKVFAGYHTIIARTLEQVNYRYMSYLFDSNDWRIQIRTKVSGVKVFSITQS
                     ILKGTKVILPDLLEQRNIAQYLDSKCKGIDSIIDKTKLQIDKLKEAKQSLISEAVTGK
                     IEILD"
     gene            6393..6845
                     /locus_tag="KPK72_002957"
     CDS             6393..6845
                     /locus_tag="KPK72_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490152.1"
                     /translation="MKLSRKNITMQVLSKFIENNLEKIENVETKANQIVFRTIDGDKK
                     IKIKTSKNYSEGEDPNTYLWTSFSSEELEEDYDYYTIICNEDIEKAVVFTKEELKQHY
                     VLETKKKKNREIDFELYPHFIGRRCIDARVHLYKEPIDITKYVNNYIF"
     gene            6879..7199
                     /locus_tag="KPK72_002958"
     CDS             6879..7199
                     /locus_tag="KPK72_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490153.1"
                     /translation="MLYVGDYIDMKTLNIRYKDGFKIEEDTRLYPINSDINDKIGVLV
                     EHRKLNATNRYLRYGIQKISGKCYFFDVRNSVISKTKESAYNTFICPYCRERMFIVSA
                     FERQ"
     gene            7275..8177
                     /locus_tag="KPK72_002959"
     CDS             7275..8177
                     /locus_tag="KPK72_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490154.1"
                     /translation="MKLRQNYYELEEFIHREIQDYVCKSAQEGFKLSIQSSYKLYKNL
                     HTNTIRADIQKERVNIVNAIPIYNTSQKNNKKYTPDIILYTDNNREIYCEISVYKSVN
                     TADYFDEWKVLGKTVIEIRKTEIMDMHLDGYRLSKDLIISYLYDPILDKARKQKISES
                     QKIIDKSNNIISKHKNLNKENNVKENNSKEKIREKEYKEFDDKVNYDIKKNLNNLNEN
                     NILIKEYVDKRTDKQKRSDKRNGREPKKELVYFDVKANKEARIIYAVIMKNSKRAYRS
                     IPAIIGKYLRDDGYKVGVAGKKRK"
     gene            8239..11220
                     /locus_tag="KPK72_002960"
     CDS             8239..11220
                     /locus_tag="KPK72_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBG2490155.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVEDLFRFL
                     EITQKELLDEFKYNRGSNYKKAFIDLINSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKQDRNPKETLFGFNQRVFVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     MGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKKSKRAIFPRFHQLDAV
                     RKIVADLEVNKAGKNYLIQHSAGSGKTNTIAWTAHRLSSLHDEDNNAIFDSVIVVTDR
                     KVLDKQLQDAIYQLEHKNGLVVKIDDDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLEKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKIIDAENEKNEKSSIDKMN
                     DLIEKRTNLDHISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPVSVYYKVGKKIEDNPEFDKGEAKRAINKFISLNEHNIRQKVETIVDDFVDNR
                     SMWLEGRAKGMIVTASRLHAVKYKLAIDQYIKEKGYNMKSLVAFSDTVKDEDIEYTEV
                     GMNKEVAPDINDSNLPSIFEKSDFRLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKHTFILDFQNSVEDIQEAYKPYFEMTNIDKVTEPNVVNDLWYQLHEY
                     GIYTDEEVNDFAVLFYKDKRTNTQDAKMNSLIDKAVERYCYLDEEDEKSADEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLRKLPKKGKERIDIDDILSLDYYKLQKL
                     GEDGKKGSDISLYNGEGELVGISETATSRVSEADEEYLEDIIQKINDVFGVGLTEEDR
                     IIIDQYEEMFKNDKSIMDIAKANSYEDLVSTFGKSYFKRGIVKTKNMNDRLVKEILTN
                     NNLQNYMIHYLAEKIYAEANR"
     gene            11238..11381
                     /locus_tag="KPK72_002961"
     CDS             11238..11381
                     /locus_tag="KPK72_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490156.1"
                     /translation="MTDKIIKPKNLFIYELKCDLIEDDERFVIKGKLDKEEINNLIFY
                     LQV"
     gene            11843..12178
                     /locus_tag="KPK72_002962"
     CDS             11843..12178
                     /locus_tag="KPK72_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490157.1"
                     /translation="MINLFKKEDLNLLLEYPKEVVENVDNVINILDESYGDNRKITDN
                     GGYVCIIEDIKEIEYLKSHILNGLIEEFSDVIYESEVDIYNSTLYLLSSDYSITIISK
                     NEETEHLLK"
     gene            12242..12703
                     /gene="radC"
                     /locus_tag="KPK72_002963"
     CDS             12242..12703
                     /gene="radC"
                     /locus_tag="KPK72_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG2490158.1"
                     /translation="MDKKKIPAKRVDIVSLKLVKESSVLYETRKISNPYDAYRLVKNF
                     LVDSDREKFVVVYLDTKNQPVSIEIVSIGTVNSAMVHPREVFKVAVLSNASKIICFHN
                     HPSGNTNFSKEDEVITKRLQKCGEILGIELVDHIVVGDDDKYFSFKENFKI"
     gene            12844..13137
                     /locus_tag="KPK72_002964"
     CDS             12844..13137
                     /locus_tag="KPK72_002964"
                     /inference="COORDINATES: protein motif:HMM:NF017894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490159.1"
                     /translation="MFKKENRYMTREIAENLLIEITILLWDLIDNLDIEKDYLQIFEI
                     NPIGLGVVEIVHRQEVPEYEASIYIQNDLIKYKLKIYAIDSIKYSTMMFSNEY"
BASE COUNT         5577 a         1317 c         2239 g         4012 t
ORIGIN      
        1 ttaaaaagat gtgtataaga acaaattcac ttaaattttt aaaatcaaat aaagttgaat
       61 ctcttgacta ccttgattgt tttatgaaat taatatagat gtaaacttta ctattaaaaa
      121 aatacaagat tttagataag ttaatgctaa ttataaataa taagataaga aatataatat
      181 gacataaggg ctaggaataa gataaatata atcaaagaat aagatatgag aataattacc
      241 aagagaaatc attaccttaa tatgtttata aatgttagac tatgttccta aattgaattt
      301 tatatatcaa gtataataac cttacagaat gtggaaattt ttatttaaag aatacacata
      361 aacatttcca cactctgatt ttataataat aatctaataa aatttatagt gactttataa
      421 aatcaataat gatttaataa atatttataa aacaactatt tttgcttaga aactttatta
      481 ttcatattat cattagtagg cattgctcca aaacttagtt tttcatacat tacttgagtt
      541 ggagctagcc aaactgtacc attaccagta aatgtattta agaaaccttc accatttaaa
      601 gcagaaccaa ttaaagattt atctgatttt gtaacagaaa aattaacacc agatgttctt
      661 gctattgcaa agttaccatc tacctttaat tcttcaccat ttttcaattc atactctaca
      721 atttcacttt gaggtacaac acattccaga acgattacac cagtaccact taattctatt
      781 tggaagaagc cttcaccacc aagtaaagca gatgagaagt tcttttgtgt gaatgctttt
      841 acttctattg tatcagagca acaatagaat aatcctttat caacaattat agaatcatta
      901 tcaagactca taaataaata atgtctaaaa gaaggttcca aataaacttc tccagtacct
      961 ttgtatacag gtttcatcat tgattcatta gtaagtgctc ctgatattcc ttttttaaaa
     1021 agacctccaa caccaccaat cttagtttca gaagatatat ttcctttata atagtataat
     1081 gcaccagctt ctgtttttat tgatgaatta tttaatttta tcttaatttg tctagctttc
     1141 aaaccagctt ctctagcaaa atataatccc attgccatta atgggctaga agctccctca
     1201 agtttttcaa attctaatat ctcaaattcg cttttgccat ctgatgcatt ttctaaaatt
     1261 tttacttttt tgcttaaatc catacaaatt tctccccact atgataaaat ttatctctaa
     1321 aatattataa gatataactc tatattacat tttttttgaa aaaatcgcta ctttatcatc
     1381 aaatttttac atatttttaa gaagtttttg aaatgtttag atttaagtta agttatttaa
     1441 ggatgtatag ttatttcaat agacattttg agtattattt atcatttgtg ggtattatat
     1501 tttatgggca tgttaaaatt atttagaaga tagaagatag aagatagaag atagaagata
     1561 gaagatagaa gatagaagat agaagataga agatagaaga tagaagattt ataatatgta
     1621 atatttttgt tttgaaattt ataaattagt attactggtt agagttagtt tagtattaaa
     1681 tatattcgaa gtagtttttt agctattttc atacatttat attacttaat tatatatagc
     1741 ctaactaact taatatatca gtgaaatatt gactaaaatg cctaaatatg ttatcataca
     1801 agttagtcaa ataatttggc aacattaaat atttagtaaa tttctaatat ttatggaaga
     1861 aatttctagc aaagaaggtg agatttatat ggctggtaaa aaaatattag caacaaacaa
     1921 aagagcaaga catgaatatt ttatagaaga aacttatgaa tgtggaattg aattaaaagg
     1981 aacagaagta aaatcaataa gacaaggcaa agttaaccta agtgatggat ttgcatctgt
     2041 agataatagc gaagtatttt taaaacaagt tcatataagt ccatatgagc aaggaaatat
     2101 attcaatgtt gattctttaa gagttagaaa actattactt cataagcatg aaataagaaa
     2161 gcttattgga gcaactacta taaaggggta ttcacttata ccacttagtt tgtatctaaa
     2221 aaacggtaaa gttaaagtag agattgcctt agcaaaaggt aaaaaactat atgataaacg
     2281 tcaagatatg gctaagaaag atgcacaaag acgtatagaa agagaaatga caggtaagta
     2341 ttaaactaga taaaactgta ttttttagaa taaaattctt tattatctag aacataacat
     2401 tgatttttat tcttaaaagt attatattat atatacatta aaaatttaat aaaaatggtg
     2461 ataaagtttg cgaaaagtct actggactat aaactactca agggggcgta aaggtttcga
     2521 cgtgggtttg gaacttgagg ctgcatgtcg tgttactctg ggtcacgtaa aaactgggga
     2581 actttaaaat aaacgcagac gataatttcg cgatagcagc ctagttgctg ctcgtccctc
     2641 ttagtcctcc tgccgactaa gactggacgt cattatgcag ggaactactt taggggtgtc
     2701 tcgacttgaa gtagattaat tgggactggc taagcataga gcctgccact tggcgctatg
     2761 tgaggtgaga tattagtgaa gttggctaag catgtagatg cagcaaggaa aagatttgcg
     2821 gacaggggtt cgactcccct cgtctccacc attgaaatcc acgaaaaaca tattgatagt
     2881 tttgtcaata tgtttttttt gtgccatgaa atgatataat tataatataa actaatttaa
     2941 aatttaatta aaaggagata gtataagtga gtatagataa taattttaat catattgctt
     3001 ttttatggaa tatagcagaa agtttaaggg gtacatacaa agaagaagac tacagaaaag
     3061 taatgttacc actaatagta ataagaagat ttgattgtct attagatgat tatgatagag
     3121 aaatagtgaa atcagtatac aaagaatatg atttcttacc agaagaagaa aaagacgaac
     3181 ttgtaatagt ggatctaaaa gaaaaccata atatagactt acagttctac aatgtgagtg
     3241 attttacatg gaaaaaactg ttagatgata gtgaaaatat aaaatcgaac tttgaagaat
     3301 acctaaatgg attctcaaac agtgtaaaag aaataatagg gaaatttaaa ttcaaagatg
     3361 aaatagctca attagataaa aaagacaagc tatatgcagt ccttagcaaa atgtatgaag
     3421 tagacctaca tataaactct gtatcaaaca acgaaatggg atacatatac gaagaaatgc
     3481 taagaagatt tacagaaaac tcagcagcag gagaacaata tactccaaga gaagttataa
     3541 gactttgtat ggaaatgtta tttatgggca aagaaaactt ccttacagaa gaaggaaaag
     3601 taatatctat agcagatttt tgctgtggta ctggtgggat gctttcaata gctgaagact
     3661 atgtggaaaa agtaaatcca agtgccatag taaatgtata tggacaagaa ttactagatg
     3721 aatcatttgc aatatgtcag gcagatatga taatgaaagg acaaaatcca gataatataa
     3781 gactaggaaa tactcttacg caagatagat ttagtggaga aaagataaga tttttaatat
     3841 caaatccacc atttggtgtc acatggaaag atgaagagaa gaaagtaaaa gaagaagctg
     3901 acttaggatt tgatggaaga tttggggcag gaacaccaag agtaagtgat gggtcgttac
     3961 tattcttaca aaatatgata agcaaaatgt atgatgatga agaaggtagt agaatagcta
     4021 taatattcaa tggatcacca ttatttacag gagatgcagg aagtggtgaa tctaacatta
     4081 gaaaatggat aatagaaaag gatttactag agggtataat agcactacct actgatatgt
     4141 tctataacac aggaatagct acatatatat gggtacttac taataaaaaa gaggataaaa
     4201 gaaaaggtaa gatacaactt gtaaatgcta gtgaatatta tcaattaatg agaaaaagtt
     4261 taggaaataa aagaaaagaa ataagtcttg aacaaataga agaaataaaa gaaatatatg
     4321 aaagatttga agaaagtgaa aatagtaaaa tatttgacaa tgaaggcttt ggatatagaa
     4381 aagtaacaat agaaagacct ttaaaactga gctttagagt aaatgaagaa gctatagaaa
     4441 atgttaaaaa tactactcaa tttataaatc tttcagtttc taagaaaaaa gatgaagaag
     4501 ttaaagttaa ggaagaagca gaaggaagag taaaacaaga taagttatta aaacttttag
     4561 aaagttttga tagtgaattt gagtatatga agagggataa gtttattaaa gatttaaaat
     4621 ctaagtctaa gttatatgat gtagctttat cagcaggact aattaaagct atagttaatg
     4681 ctataggagt aagaaatgaa gacgcagtag tttgcaaaga tgctaaaggg aatatagaga
     4741 gtgatagtag tttaaaagat actgaaagta tagcacttaa agaagatgtg tatgagtatt
     4801 ttgaaaaaga ggtaaaacct catgttgaag atgcttatat agatgaaagt agtatagata
     4861 atataggata tgagatacct tttactagat atttttataa gtatgaaaag ttaaaaagct
     4921 ttgatgatat tatgaaggaa gttgaaagtt tagaaagtga gatagcactg gagataagga
     4981 aggtgctagg gtaatgagat atagatatag agatgatgag gaaatgaaag atagtggagt
     5041 tgagtggata ggtaagattc ctaaggattg ggaagtaaaa agaataaaac atttatttga
     5101 attaaagaaa gataaatctg atgaagaaaa tccaacggta ttatcattaa ctcaaaaagg
     5161 attaaaaata agagatgtaa gtaataatga gggacaatta gcatcaacat atgtaggata
     5221 cactaagatt gaaaaaaatg attttatact taatccaatg gatttaatat caggatatac
     5281 tgataaagca gaaattgaag gtgtaattag tccggcatat actaccttta gaagtaaaaa
     5341 taaagtaaat attaatcacg attattataa aagatatttt caaatgcatt atcatcataa
     5401 tttcttattc ccgtggggag agggggtatc attcgaacat agatggacat taaaaaatga
     5461 agtattttta aatcttccag taataacaaa taggttagaa gaacaagaaa aaatagctaa
     5521 tttccttgat gaaaagactt cacagtttga atttattata tctaagaaag aagaattaat
     5581 aaaaaagtta gaagaagcta aaaagagcct tataagtgag gttgttactg gtaaggtgaa
     5641 ggttgttaag actgatgatg gatataagct tgtgaagaga agtagtgaag aaatgaagga
     5701 tagtggaatt gaatggttag gtgaaatacc taaggattgg gaagtgaaga actttaagta
     5761 tatgtttact ttaaataaag gtttatcaat aacaaaggca gacttaaaag aaagtggaat
     5821 accgtgtgta aattatggag agatacactc aaaatatagg tttgagctta agcctagtaa
     5881 acataaatta aagtacgtag atgaatcata tttaaaatcg aattctattt cattattaaa
     5941 gtacggagat tttgtttttt gtgatacatc agaagatata gaagggtgtg gtaattttac
     6001 atatttaaat gaaaataata aggtgtttgc tggataccat actataatag ctagaacttt
     6061 agaacaagta aactatagat atatgtcgta tctatttgat tcaaatgatt ggagaataca
     6121 aatacgcaca aaggtatcag gggttaaagt ttttagtata actcaatcaa tattaaaggg
     6181 tacaaaagta atactaccag atttattaga acaaaggaat atagctcagt atttagatag
     6241 taaatgtaaa gggatagatt ctattataga taaaactaaa ttacaaatag ataaactaaa
     6301 agaagctaaa caaagtttaa taagtgaagc agtgacagga aagatagaaa tactagacta
     6361 actcttagta tatttgaaca gaggtgaaaa atatgaaatt aagtagaaaa aatataacaa
     6421 tgcaagtttt atcaaaattt atagaaaata acttagaaaa aatagaaaac gtagaaacaa
     6481 aagcaaatca aatagttttt agaactatag atggtgacaa aaaaataaag ataaaaacaa
     6541 gtaaaaatta tagcgaagga gaagatccta atacatattt atggacaagt ttttctagtg
     6601 aagaattaga agaagattat gattattata ctataatatg caatgaagat atagaaaaag
     6661 ctgtggtatt tacaaaagaa gaattaaaac aacactatgt actagaaact aagaaaaaga
     6721 aaaatagaga aattgatttt gaattatatc ctcattttat aggaagaaga tgtatagatg
     6781 ctagagttca tttatataaa gaaccaatag atataactaa gtatgtaaat aactatatat
     6841 tttagtaata tatataatca ataaaattat attttacatt gctttatgta ggagattata
     6901 ttgatatgaa aacattaaat ataagataca aagatggatt taaaatagaa gaagatacga
     6961 gactttatcc tataaatagt gatataaatg ataaaattgg agttcttgta gaacatagaa
     7021 aactaaatgc tactaatagg tatttaagat atggtataca aaaaatttcg gggaagtgtt
     7081 atttttttga tgtaagaaat agtgtaatat caaaaacaaa ggagagtgca tataatactt
     7141 ttatatgtcc ttattgcaga gaaagaatgt ttatagtatc agcatttgaa agacaatgaa
     7201 gcatagtacc aattcacttg aaacattata aaaagacaaa taaataatgt atatttagaa
     7261 cagattctaa aagtatgaaa ctaagacaga attattatga attagaagaa tttatacata
     7321 gagagataca agattatgta tgtaaaagtg cacaagaagg ctttaaatta agtatacaga
     7381 gttcatataa attatataaa aatctacata ccaatactat aagagcagat atacaaaaag
     7441 aaagagttaa tattgtaaat gctattccaa tttataatac aagtcaaaaa aataataaaa
     7501 aatatacacc ggatataata ctttacactg ataacaatag agaaatatat tgtgagatat
     7561 cagtttataa atctgtaaat acagcagatt attttgatga atggaaagtt ttaggtaaaa
     7621 ccgtaataga aataaggaaa actgaaatta tggatatgca tttagatgga tatagacttt
     7681 ctaaggattt aataatatct tatttatatg accctatttt agataaagct agaaagcaaa
     7741 aaataagtga atctcaaaag attatagata agtcaaataa tataatatct aaacataaaa
     7801 atttaaataa agaaaataat gtaaaagaaa ataattcaaa ggaaaaaata cgtgaaaaag
     7861 aatataaaga atttgatgat aaagttaatt atgatataaa aaaaaactta aataatttaa
     7921 atgaaaataa tattttgatc aaagaatatg tagataaaag gacagataag cagaaaagga
     7981 gcgataagcg taatggacgt gaacctaaaa aagaattagt atattttgat gttaaagcaa
     8041 ataaagaagc aagaattata tatgcagtaa ttatgaaaaa tagcaaaaga gcttatcgaa
     8101 gtatacctgc aataatagga aaatatttaa gagatgatgg ctataaagtt ggtgtggctg
     8161 gtaaaaaacg taagtagtta atgattaata taagctttat aatatataga ttaacaatat
     8221 agaaatgggg gaatagcaat gttaccaaaa gagaaacatt tagaagagtc tattgaaaat
     8281 catttagttt caattggata cgaacaaggt cattcacaag aatttgatct tgaaaattgc
     8341 ttattcgtag aagatttatt tagattttta gaaattactc aaaaagaatt actagatgag
     8401 ttcaaataca acaggggaag taattacaaa aaagccttta tagatttaat taactcaaac
     8461 atcaaaagaa gggggcttat aagtgtactt agaaatggtg tagaagatat aatgatgaac
     8521 ggaaaattcc aattattcta caacaaacca aatctaacta ctaacaaaac agcttacgaa
     8581 aattataagc aaaatatatt caaaataaca agacaattaa aatatagttc aaagcacaac
     8641 aatactttag atatagtatt atctttgaac ggatttcctg ttatagtaat ggaacttaaa
     8701 aaccaattca ctaatcaaaa tgtgtataat gctataaaac aattcaaaca ggatagaaat
     8761 ccaaaagaaa ctttatttgg atttaatcaa agagtatttg tatattttgc agtagatact
     8821 gacgaagtat atatgacaac agagttaaaa ggagataaaa cattcttctt accttttaat
     8881 aaaggtaaca atatgggtaa gggaaatcct gtagtagaag gaaaagaaaa aacttcttat
     8941 ctttgggaag aaatacttac taaagatagt ttacttgata ttataaagag attttacttt
     9001 atccaagaag atgataagaa aagtaaaaga gctatatttc ctagatttca tcagctagat
     9061 gcagtaagaa agatagtagc ggatttagaa gtaaataaag caggtaaaaa ttatcttatc
     9121 cagcatagtg caggtagtgg aaagactaat actatagcat ggactgctca tagattatca
     9181 agccttcatg atgaagataa caatgctata tttgattctg taatagtagt tacagataga
     9241 aaggtacttg ataagcaact tcaagatgct atataccaat tagaacacaa aaacggatta
     9301 gttgttaaga tagatgatga taaaaattca tcagatttag ctgatgctat tgtaaacaga
     9361 gcaaagatta ttattacaac tatacaaaag ttccaatatg cattacctaa gatagaacaa
     9421 cttgaaaaaa gacgttatgc agtaataata gatgaagctc attcttctac atctggtgag
     9481 aatatgaatg cattaaaaga aactttagca ggaaaaacat tagaagaagc taagataatt
     9541 gatgctgaaa atgaaaagaa tgaaaaatct agtatagaca agatgaatga ccttatagaa
     9601 aagagaacta atttagatca tataagtttc tttgcattta ctgctactcc taagaataag
     9661 actatgcaag tatttggtag aataggagaa gatggactac ctcatgagtt tcatttatat
     9721 tctatgaagc aagctataga agaaggattc atattagacg tattaaagaa ctttatgcca
     9781 gttagtgtat attataaggt aggtaaaaaa atagaggata atcccgaatt tgataaagga
     9841 gaagctaaaa gagctataaa caaatttatt agtttaaatg aacacaacat acgtcagaaa
     9901 gttgaaacta tagtagacga ttttgtggat aataggtcta tgtggttaga aggcagagct
     9961 aagggaatga tagttacagc aagtagatta catgctgtta agtataaatt agcaatagat
    10021 caatatataa aagaaaaagg gtacaatatg aaatccttag ttgctttttc agatactgta
    10081 aaagatgagg atattgaata tacagaagtt ggtatgaata aggaagtagc accagatatt
    10141 aatgattcaa atttaccaag tatttttgaa aaaagtgact ttagattact tatagttgca
    10201 gaaaagtatc agactggttt tgaccaacca aagttatgtg ctatgtatgt tgataagaag
    10261 ttagatggtg taaaaactgt tcagacttta tcaagactta atagaacata tcctaataag
    10321 catacattta tcttggattt ccaaaatagt gtagaggata ttcaagaagc atataaacca
    10381 tactttgaaa tgacaaacat agataaagtt acagagccta atgtagtaaa tgatctttgg
    10441 tatcagttac atgaatatgg tatatatact gatgaggaag taaatgactt tgcagtatta
    10501 ttctataagg ataaaagaac aaatacacaa gatgctaaga tgaatagttt aattgataag
    10561 gcagttgaaa gatattgtta tttagatgaa gaagatgaaa agtcagcaga tgagtttaag
    10621 aaaaaatgtc aaaagtatat tactttttat aatttcttaa tccaaattta ccctataaag
    10681 aacttaaatc ttttaaggtt acaggtttac ttagtagcac ttttaaggaa gctacctaaa
    10741 aagggtaaag aaagaataga tatagatgat attttaagtt tagattatta taagttacaa
    10801 aagttaggtg aagatggtaa aaaaggtagt gatatatcat tatataatgg tgaaggtgaa
    10861 ttagttggta tatctgaaac tgctacttct cgtgtaagtg aagctgatga agaatattta
    10921 gaagatataa tccaaaaaat aaatgatgtc tttggagtag gacttacaga ggaagatagg
    10981 attataatag atcaatatga agagatgttt aaaaatgata agagtattat ggatatagct
    11041 aaagctaaca gttatgaaga tttggttagt acttttggaa agagttattt taagagaggt
    11101 atagttaaga ctaagaatat gaatgataga ttggttaaag aaatattaac taataataat
    11161 cttcaaaatt atatgattca ttatttggct gaaaaaatat atgcagaagc aaatagataa
    11221 ataaaaagga gtacaatatg acagataaaa taataaaacc taaaaattta ttcatatatg
    11281 aattaaagtg tgacttaatt gaagatgatg aaagatttgt aattaagggt aaattagata
    11341 aggaagaaat aaataattta atattttatt tacaagttta gtttagaaat atattagata
    11401 attccatatt aataccatac gatataagta atatattaac tagactatat gggtttgagg
    11461 aagtacaaaa tattgaagat atatcaatag atcaaacgat agaattaaca gataatatag
    11521 atgaaaatat atacaaaata gatcaaatat tagaaaatag atcaaaccta tatgtggatg
    11581 ggttatatga tatgctagaa ggtataaagt tagagagtgt acaagataat ccgactgtat
    11641 ttgaagaaga gtatgtaaaa cttcaaaata aataaggaat ctagtaaaat gtaataatta
    11701 taattatata ttataatttt gaaatataca ttaaatagat atcatactaa agaggaacat
    11761 acaaaaaata acacattgag atgttggtag agatactaac atctcttttt ttagttcaaa
    11821 aaattataag gaggaaatta aaatgattaa cttatttaag aaagaagatt taaacttatt
    11881 attagaatat ccaaaggaag tagttgaaaa tgtggataac gttataaata ttcttgatga
    11941 atcatacgga gataacagaa aaataacaga taatggtgga tatgtgtgta taattgagga
    12001 tattaaagaa atagaatatt taaaatcaca tatattaaat ggattaattg aagagtttag
    12061 tgatgtaata tatgaaagtg aggttgatat ctacaattca actctatatt tactatctag
    12121 tgactatagc attactataa tatctaaaaa tgaagaaact gaacacttac ttaaataagc
    12181 atatacattg ataaagtgta tatgcttatt ttaattttga gaaacaaagg aggaaataat
    12241 catggataag aaaaaaatac ctgcaaaaag agttgatata gtatcactta aactagtaaa
    12301 agaaagtagt gttctatatg aaactagaaa aataagcaat ccttatgatg cttatagatt
    12361 agtaaagaac tttttagtag atagtgatag agaaaagttt gtagtagtat atttagatac
    12421 aaaaaatcaa ccagttagta tagaaattgt aagcatagga actgtaaatt cagcgatggt
    12481 acatccaaga gaagtattta aagtagcagt attaagtaat gcatctaaaa taatatgctt
    12541 tcataatcat ccatcaggta atactaactt tagtaaagaa gatgaagtta taactaaaag
    12601 gctacagaag tgtggtgaaa tattaggtat tgaattagta gatcatattg ttgttggaga
    12661 cgatgataag tattttagct ttaaagagaa ttttaagata tgagagtctt agcttaaaga
    12721 aattaggcta agactttttc tatgttctaa actaattgaa ataaattagt tttaagttaa
    12781 gcccgaaaag cccttgaaaa aataatatac atataaataa ttaaactaaa taggagttgt
    12841 aaaatgttta aaaaagaaaa tagatatatg acgagagaaa tagctgaaaa tctactaatt
    12901 gaaataacta tattactatg ggatttgata gacaacttag atatagaaaa agattatcta
    12961 caaatatttg aaataaatcc tataggatta ggagtagttg agatagttca tagacaagaa
    13021 gtacctgaat atgaagctag tatatacata cagaatgact taattaaata caaattaaaa
    13081 atatacgcta tagatagtat taaatacagt actatgatgt tttcaaatga atattaacta
    13141 ataat
//
LOCUS       DAEMHZ010000119        12982 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000119 DAEMHZ010000000
VERSION     DAEMHZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.119"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..553)
                     /locus_tag="KPK72_002965"
     CDS             complement(<1..553)
                     /locus_tag="KPK72_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490160.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDI"
     gene            complement(754..966)
                     /locus_tag="KPK72_002966"
     CDS             complement(754..966)
                     /locus_tag="KPK72_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG2490161.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(1039..2754)
                     /locus_tag="KPK72_002967"
     CDS             complement(1039..2754)
                     /locus_tag="KPK72_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2490162.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(2754..3308)
                     /locus_tag="KPK72_002968"
     CDS             complement(2754..3308)
                     /locus_tag="KPK72_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG2490163.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(3301..3903)
                     /locus_tag="KPK72_002969"
     CDS             complement(3301..3903)
                     /locus_tag="KPK72_002969"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG2490164.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(3912..6560)
                     /gene="polA"
                     /locus_tag="KPK72_002970"
     CDS             complement(3912..6560)
                     /gene="polA"
                     /locus_tag="KPK72_002970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG2490165.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDIDLNVGSSWYETK"
     gene            6712..6799
                     /locus_tag="KPK72_002971"
     tRNA            6712..6799
                     /locus_tag="KPK72_002971"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6746..6748,aa:Leu,seq:caa)
     gene            complement(6986..7429)
                     /locus_tag="KPK72_002972"
     CDS             complement(6986..7429)
                     /locus_tag="KPK72_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490166.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(7432..7629)
                     /locus_tag="KPK72_002973"
     CDS             complement(7432..7629)
                     /locus_tag="KPK72_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490167.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(7821..8687)
                     /locus_tag="KPK72_002974"
     CDS             complement(7821..8687)
                     /locus_tag="KPK72_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2490168.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(8913..9782)
                     /locus_tag="KPK72_002975"
     CDS             complement(8913..9782)
                     /locus_tag="KPK72_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2490169.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(10288..10509)
                     /locus_tag="KPK72_002976"
     CDS             complement(10288..10509)
                     /locus_tag="KPK72_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490170.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(11010..11990)
                     /locus_tag="KPK72_002977"
     CDS             complement(11010..11990)
                     /locus_tag="KPK72_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490171.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(12154..12951)
                     /locus_tag="KPK72_002978"
     CDS             complement(12154..12951)
                     /locus_tag="KPK72_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2490172.1"
                     /translation="METTEYTIFNKKIPKEFDNFKIVQVSDLHNKVFDKNNKVLIDKI
                     ESLNPDVIFITGDLVDGENKNFQVALDLIDNLVEKYEVYHIIGNHEQKSLIKKYKHLY
                     KDYFKELYSKKIINMENESVRIYRGNSHLNLYGLIIPLECYPYLFANNKKLELSDNFV
                     EDNLGKVNENEYNILLAHTPFFFEKYAKWGADLVLAGHVHGGIIRIPFVGGVLSPNRE
                     FFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLNCKPEIVKITLKYEK"
BASE COUNT         4098 a         2276 c         1215 g         5393 t
ORIGIN      
        1 taatatcttc agcattataa tcatcttcta cttctatatc caaaagtttt gctattgacc
       61 tcatatcttc tctagtcttt gacctatagc ttgatattaa ttcatctttt attttctctg
      121 gttctaaaag atattcttca tctcctcttt tgaaaacaac gtatttttga gcatatgaac
      181 ttctattgta gtatatatat cttgatattt ttcttaaaat ttgattatat gaaagtcgct
      241 tgttaatatc aagttttaag tactctgaaa aagcaaatag ctcttttttt gatttaaata
      301 atgcttcttt ctttatagtc cttgatattg aagttacact ttcttgatat gcacactttt
      361 ttaacatttt taaatatact ctattaagac caatactttg attgaatttt tttatattat
      421 tattaataat cttatccaag ttaccttcaa agtatttttt tgctcctttt tgtgatacat
      481 aactctcaag ttctttgtgg gccatatcaa gtatcatttt ttttaaatcc tctttatatc
      541 ccctagattc catgatactc cctcccttag ctatttatag tatattcaaa aagctcaaga
      601 aatgtgagtc attacaaaat ttttttactt tttatgtata tattaattta aaaggggaga
      661 taatagtaat gtaaaatcaa acatactcaa tctcccccat ttttagacct ctattactcc
      721 cccaatagag gtcttttttt agtttatcaa tttctattca attcccttta gttcaaatcc
      781 taattctgct atcacttctt ttaatttttc aatagataca tcatctttca tatctacaga
      841 tgcacactta ttttctaaat ctacatctaa aacttcaata ccatctatat cttctgttaa
      901 tgcagtttta agatggttta cacaatgacc acaactcata ccttctacta atagtttctt
      961 cttcatttaa ttacctcctt ataccctata agggtattct atcatatagt ttatactttt
     1021 taaatagaaa ttaatcattt aatttgattc attcgatttt atatctaaat tagatattcc
     1081 ctcatataca tcaaaatcat ttggagtaat tttacctttt aatctactat tacttacaat
     1141 atagttgttt ctcacagcta aacttataaa tgttgcatta tcttttatat atgtctgtac
     1201 atctttgtat gctttttttg tttcaatttc agacgtagaa cttgtaagtg cagacatata
     1261 tccatttaac ttatcatcag tatatccact actcgctata atactacttg catctggtat
     1321 aattgatagt ttccatccaa ctaatgctaa atcataattt ttagaattaa gagcattttc
     1381 catatcctta tctgacagct gatttactgt actatttatt cctatagctt ttaagttttc
     1441 atttattatg tttgcagttt ttattctctc actattatct ctgtttacta ttatctttaa
     1501 gttcatttca gaaagacttt tctttacttc ctctttttct ctattttttt tctcttcagc
     1561 ttcttttttg attctatctt cttctgcttt tttagctttt gcttcttctt ctggcgtaag
     1621 cttctttgag ttctttttag ccgatttttt ctgatttttt actttattgt cattttgatt
     1681 tactgagttt acatttgcat attctacctt agcaagacac tctatcgcct tatctttatt
     1741 aaactctaaa tccttcattt cactattata gtattttgat tttgaattaa gtggaaaatt
     1801 aactggagta gcatcatcca tataaccttc actaataatt ctatctttat ctatagagtg
     1861 tgcaattgct tttctaaaat taacatcttt gaaaaaagga ttattataat taaataatac
     1921 acactcataa tctcttcctt gatattttgt tatattaaac tctttttctt gaaacttact
     1981 taaatcattt aatgtaacat tggttatatc actatcaaga gccataacca tagatgtcct
     2041 tgcatcttca ttaggcacca tacctacttt tatgttcttc atggtctttg gaacttcttc
     2101 atagtaatct ttgtttacag ataaaaccat tccttctctt tctgtatatg attctatttt
     2161 atatttccca tttcctatca tattattatt cttgtcattt acatcttttg aatctaaatg
     2221 attttgagat acaattggaa aaattaaact atcaattgaa aaagcatatc tagctttaaa
     2281 ttttattgta aagtctttat ctgatttaat attcacagaa gctatatttt tagtaaattc
     2341 attatatgga ctatctgcac ttttttgaat taaaccaata gtatagttta catcatttga
     2401 agtaacattt tttccatcat gccactttgc atcttttagc tttatatcta tactcattcc
     2461 atcctgagct atattatact ccttaactaa ttgaggaatt acattgtagt tctcatctat
     2521 agtaaataaa ctatcataaa ctaaattcat tatataacca acagacttat cagtattagt
     2581 tataggattt atagtttttg gagttaccat tgttaagttt atatacttag catctataga
     2641 actttgactt tgtaaacttg aatcactact actttgtttt gttttagtat tactacaacc
     2701 agctaccata agagttatag ctaaaacgac tgttaagact tttattctct tcattagttt
     2761 tttcctttct tgccatatat cttattataa tgttcgtaat tattttttac tttttctaca
     2821 tattttgaag tttgcttaaa aggtatatca tgtagatttt cgccatcttt actatattca
     2881 ttattttcta accatttttt cacattacct gaaccaccat tatatgcagc tatcactaaa
     2941 tctaatttac caaattcttt gtataactta cttaaatacc aacagccaag tcttatatta
     3001 gtctctgggt cgaaaatatc cacatttttt aaatttagtt cctctgctcc ccaatctcta
     3061 gttatgtcta atatttgcat taatcctttt gcttcctttt ttgaaacagc agaactatta
     3121 aacttacttt cggctttaat aacactgtaa actatatttt catctaaatt aaattctttc
     3181 gaatactttt ctacatactc tgaatatttt ttaggatata aaaatttatg tattactttg
     3241 ctttccatta atagtgcccc aaataagatt ataaaaatag atagaatcaa tactttctta
     3301 ctattcacgc caattctcct tcatgtactc aataaattta tgtgctttac tttctaattc
     3361 agttattgtt cccgaattat ctattatata atcaccatat ttggattttt cttcctgact
     3421 catctgtgaa tttatcctac taagtgcttc ctgctcactg caattatctc tcttttgtat
     3481 tctactaatt tgtacttctt gtttacaagt aactactaat agcttgtcaa ccatatctaa
     3541 aaaaccactt tctaccaata ttgccgcatc taacactact atattttcgc ctttttttct
     3601 caatttttct atttcactta ttattttttc tcttattcgt ggatgagtca agttatttaa
     3661 ttccttaagt ttttcttcat cactaaaaac tattttccca agagcttttc tatttagagt
     3721 tccatcatca ttttttatac tttgcccaaa atggacaaat actttttcca ataacaactt
     3781 atcctcaaat atcttcctgg atataatatc agcatccact ataggaatgt taagatttct
     3841 aaatatattt gataggctgc tttttccaca gcctattcct cctgtaagtc ctaatatcaa
     3901 cataaaatca cctactttgt ctcataccaa gaactaccta catttaaatc tatatctaag
     3961 tgaacatcca tatttacagc attttccatt tcttctttta ctattttctt aactatatct
     4021 atttcatcat caattgcttc aactatcaat tcgtcgtgaa cttgtagtat aagttttgat
     4081 ttcaaattgt tctcttccaa ttttttatga acatttatca tagcaatttt tattatatct
     4141 gctgcacttc cttgtattgg tgcattcatt gcaaatctct ttcctctatt tctttccata
     4201 aaattgcttg actttatttc tggtatatat cttcttctgt ttaaaattgt tacagcatat
     4261 ccatcctcac tagccttttc tactgttgtg tccataaact cttttatttt atggtattta
     4321 gcaaaataac tttcgatata ttctttcgcc tttggaacag gtatatttaa atcatcagct
     4381 agaccaaagt cacttttacc atatataata ccaaagttta cagctttagc tgcacctctc
     4441 aattcactag taacatcttc cataggaaca ttaaaaactt gtgaagctgt tttggtatga
     4501 atatcttcat gatgtttaaa tgcttctatc atattttcat cctgactcat atgtgcaaga
     4561 actcttagct ctacttgtga gtaatcggca tcaaccaact tacagttatc atctgcaata
     4621 aacacttttc ttatttttct acccatttcc atttttatag gtatattttg aagatttggt
     4681 tctgtagatg aaattcttcc tgtagtcgtt atagtttgat taaatgatga atgtattcta
     4741 ttactttctg gatttattat atttaaaagt ccttcaacgt aagtagaatt taactttact
     4801 atttgtctat actcagttat cttatctatt attggatgct tatctcttaa tttttctaaa
     4861 acatctgcat tagttgaata tccagtttta gtttttttaa ttattggaag ctctaatttt
     4921 tcaaataata taacacccaa ttgttttggt gaatttatat tgaagggctc tcctgataat
     4981 tcataaattt ccttttctag tgtagctatt atttctttaa actctacact caattcatcc
     5041 aatatacttt tgtctacttt tataccttca tactccatat gacctaagac ttctactaaa
     5101 ggcatctcta catgataaaa aagaccatcc atactcatat ccattagact tctttccatc
     5161 ataggaactg tttctttaac tgtgcatatt atttgaccca tatactctgc taaatcctca
     5221 aattctaaat cctcatagtt tttagattta acaccctttc ctaataattc ttcttttgat
     5281 ttaacctttt ttgataaata cttcatagct attgcactac aatcatatga ggtagatgaa
     5341 gttgaatcta ttaagtactc agctattgtt atatcaaaat acatattttc taagtttata
     5401 ttatatggtt ttaaagatat ataatcatct tttaaattat atccatactt tttaatttca
     5461 ttatttgaaa atatactatc taactttgac acttcatttg aatctacata atatattttt
     5521 tctccatcta cacttataaa aatatatatt atattttttt caagtatatt accttgtcct
     5581 ctaacagttt ttaaaataac ttgtcctttt ttgtttactt catctataaa tttttctgta
     5641 tcttttaatt ttaatatttc tattttttct tctacgtgtt ctgtgtcatc tgaatcgacc
     5701 aaatcaacta atcttgataa gagacttgta aatccaaagt atctaaattt atctaaaagt
     5761 tcatctttat cataatcgcc aaaaatcata ctatctaaat ccacttctat tggcacatct
     5821 cttataattg ttgcaagtct tttactttga atagctattt ctttgttttc ctctattttc
     5881 ttttttacac tacctttaag cttatctgta ttctctatta gattctctat actagaaaac
     5941 tcttttataa gctttatacc tgttttttca cctattccag gtactccagg tatattatca
     6001 gacttgtctc ccataagacc ctttaaatcg ataaattgtg ttggagtcat ctcatatttt
     6061 tcaattacag aattaaagtt gtattcttct acttctccaa cccctttttt cgttataagg
     6121 gtagttgtat tatctgaagc aagttgaata gcatctttat ctccagttac tatgtatacc
     6181 ttatatccat tttcttctgc ttttttagaa acagtaccta taatatcatc tgcctcataa
     6241 ccatctattt ccaatctatt tattctaaat ttatctaaaa ggtcttttaa tggctgtaat
     6301 tgttctgcca gttcatctgg cattcccttt cttccagcct tatactcttt atattctaaa
     6361 tgtctaaatg taggtgcttt tctgtcaaat gctacactta tatgtgttgg tttatatata
     6421 tctatcattt taaatagcat tgtagtaaac ccatatatag cattggtctt taacccttct
     6481 ttattattca tttctggaag agcataaaaa gctctattta ttattgaatt tccatctatt
     6541 ataattaatt ttttttccaa attatatcac tcctaaaaat tatctatttt tgtaacatat
     6601 atattatata ccaatatagt gttatttaat attcccataa taataatagt atattaaaat
     6661 taaatatgtt gcaaacaata tatttttttg ctataataat atacatgaca tgcccgagtg
     6721 gtggaattgg tagacgcagt ggactcaaaa tccaccggac taatactccg tgccggttcg
     6781 agtccggcct cgggcaccat taaaaactcc taaagctatt tattcaatag attaggagtt
     6841 tttttatttt tgatttaaat tttaagttat tttttgaaat tactgtgtct ttttaaaatt
     6901 tatataatta tataaattta tcttctagca ttaatttaaa aactaattta cttttatata
     6961 attttctatt ttatatcata acttattaca accttttatc taatttattg tatacaacaa
     7021 cactacaaat aaattgaaat aaaataagca atccaagaac aactaaaacc cacttgctta
     7081 ttaccattac agaaaataca ctcaatatag aaacaataaa tataagaatt acaagaacat
     7141 agcacatgat ttgattagta atacgtccag ctttatcacg taacataatt cttctttcat
     7201 cattcatctc tatcctttga ttttttatct tttcttcata atctactgct ctttcaggtt
     7261 tactccaata catatattga agaatcatac atacacctgg acctaaagca gctccagata
     7321 atccccaaat aaaagcctca aatgaaaact cagtaaatgt agccaataaa acaagtaaaa
     7381 ttccacaaat aaaatatata attccaacat ataacatatt ctttttcatt tttactcctc
     7441 ctcataaata aatatttctt caatactcat atcaaagtat ctagctatct taaaggctaa
     7501 gatgatagaa ggattatacc taccattttc taatgaacct atagtttgtc ttgatacttg
     7561 caaaatagaa gcaagttctt cttgttttat gcctctttct tttcttattt cttctaatct
     7621 atttttcaca tctatccccc tttaatatct caactatgga aagtctactt tccataatta
     7681 tacaactata ttccatttat gtaaagctta ctttccatat ttacaaaaaa ataacagact
     7741 attttcagcc tgtaatttta gaatttcatg ttgtttatta attttaatgt tatttattaa
     7801 ttccatatta tttattaatt ttaacttggt ttctgaattg gaatccaaac ttctgtagta
     7861 taatcaggag cattgatatc gccattggga taaagttcaa tatctggtaa attagcatac
     7921 acatacccag aacttggtaa ccactcagta ataattcttt tttgcaattc ctgtattgcc
     7981 agagcatttg gcattggaga aacacattca aatactgccc atgtgcctga tggtattaag
     8041 tattcatgag tatcttctaa tacaggctta tctgttggag cagcaatata ataatctaaa
     8101 cccttactat ttgtacacac actaactcca agcaatccac aaggttcgtt atttattaac
     8161 tctgaaagtt tatcaattgt tccatttttt atgcatttag cccataactt aggaactttc
     8221 tcaaaacact cttctacaat catgttcata ctttctttta taccaacaat acgaaaccct
     8281 ttcttttcta caatacgata ttgcatttct atatttcctt ttacataaat gcttaatgtc
     8341 atcttaggat atgcttttaa aacaacaccc tttgctctag ctgcagatgg agaaatgtta
     8401 tgtatatttt gaaaagctct attaaaagat gttggtgaat cgtaaccata cttctcagat
     8461 agttctaaaa ttttgatgtt actgttctga agttcaaatg cagccttagt catacgtctt
     8521 cgtctaatat attcagataa tggtacttct gcaatatatg aaaacattct ttgaaaatga
     8581 aaggttgaac aacatgcaat ttttgctgct tctgcataat ccactccact ttcgagattt
     8641 tcttcaatat aagtaatagc ttgatttaat ttatttatcc attccatttg atattacctc
     8701 caagcaaatt atacagttta ataaaataat atacctctct attcatgcac aaaattgtaa
     8761 gcatttaaat ttataagcat ttttcaatca atacaataaa tataaaaatt gtattataca
     8821 ataaaaaagt tcaataacat tatattataa aatgttattg aactttttat aaaaattaat
     8881 ttaagtgtag ttaaattaag tattacacta tcttatatat tacaatatcc tactatctta
     8941 tagtcttctg gcactttata atctttgtct ggagtaccta aattccattt taaatcaaac
     9001 atagttgcac ttattattgt tgcaaaataa agcataacta taccaatgtc tattttttct
     9061 tcatataaat tagtatgacc atcttttcta acagctaaaa ctacagtgcc tccatcaact
     9121 ataaatctcc atggttgtct atttaatgta gatggagcta atcttgcata gtgaaatgca
     9181 tcaagaagtg ctctttcttc taagatttct atacttgcaa catttcccca ttcgttcata
     9241 taaacaacat cttctacacc tagtctagta gaccccttat ctgttacaac ttgcatatca
     9301 gattttgaat aattttgccc agtttttgta gcatttaata ctttagcatt acttacattt
     9361 tctccatatc caatagctat aattcctgtt acctctttat ctgaattaat tccaagtttt
     9421 tcttttacct tttttccatc ttcaaaagta acccaacaag aaccaattcc aagttctgtt
     9481 actttaaaga ttatactttc tcctatgtac cctgaatttt caatgtaatt atcttttatg
     9541 tcagataata ttataatata acttggagaa tctatcatat ttcctttgta ccctgctact
     9601 ttatctaaat tattaaaaac ttcacttcta ttcataattc ttacatcaac atctatttct
     9661 ggaactagtt tcttacaaga ttctgagtat ttcttaattt cactaagata ttcttcttta
     9721 atatctgtgt tcttatattc tctaacagac tttctattta atatgattgt tttataatcc
     9781 ataattttat cctccccaaa ctagtattgt cttaattata tacccattaa tatattattt
     9841 tactcattta tttaataaat aaagattaca attttgtaat tattatttat taataatatt
     9901 ccacttttac attaataatg accagttgtg caaaatccat tgccaaccac caaataaaaa
     9961 catattataa aggtaatata aaataacaaa caaatcttaa caaaaaatta aacttttttt
    10021 acacgaaaga aggcttcccg ttgccttctt tatttttata aaaaaataag ctgtttctaa
    10081 aatgatttca catttttgaa acagccaagt tattctataa tgacaactat aatttgagga
    10141 tgtgagggag agttgtcatt ataaatttag ggtaattata ttatgtttta agctagttct
    10201 ttctatattt atataataca ttttatatat gaattttata tgagattggt ataaatgata
    10261 tatgaaatta catatgttta tattatatta aattttatta ttacattttc cacaacaata
    10321 ctcatatgtc tccacattat ttggaatatt tacaaataca aattctttac agttctcaca
    10381 atactttaat atagatatag ttccacaagt gtaacattca aatattacac aatcctcatt
    10441 ttcctctact atttcaaact ttttactatg ttcaccacac tctgggcact tgtgattatt
    10501 tacattcata aatactcctc cttattagta tagttacatg tcaaaaactt aattaatgta
    10561 ttctatatta cttattatat attctataat aaatcatatt ctaatataat aaaacaaatt
    10621 aaataaaaaa aaaccataaa ttttaaattt atggattaat tcttgtaatg ataactctat
    10681 ttgagaatgt gagggagggt tatcattaca atagggtatt atattatggt ttttaagcta
    10741 gtctttctac acctcatatg atacatatgc tatatgaatt ttatatgatt tttacataaa
    10801 tattttatga aatttaaatt ttttagtcat tttgaaattt gtaaaaagtt ctctaaaaat
    10861 ccacagcatc ataaacagcc aaaatttaag taaaacatca taaaaaaatt cgtccaataa
    10921 ttttaacaag taatctcacc tcattataat tattgacaca aacttaaata aacaatctat
    10981 aaatcttcta taaatcttaa actttccccc tattgataca caatttctag tttgtcaatt
    11041 ttattttgca tttctaagaa aatttcttca acagaattgt taaatatatt gcctaaatag
    11101 attcttttat ctttttgaaa tgaataagat tttacactac catcagaaga aatatataaa
    11161 tgactccact ctgctccata ttttgaaaac ctctcctttg tatctttaaa cctcatatct
    11221 tctattttta ttgctttttc tctatctcct cttactacag tgtgacccct ttttattaaa
    11281 ctggttattt ttatatcttc ttttaattta atacgtgtat catcattttt tgaatatact
    11341 ttccatatca ctttatgacg ataattatct aattccttta ttaagcttct atcaatagta
    11401 aaactatctt tagttaccac aattaaatca aaatggtcta acatatattg aaatatttct
    11461 ctcatacttt tattttttaa aatatcctca ccaataacct ctataaatct acagcctttc
    11521 tcatacatgc agttcaataa acttgtgatt tcagaaaaat aatcatttgt attattatct
    11581 ccatctattt ctatagtaaa gtacaaagga gtaatgtatg atttatttcc tgttatctca
    11641 aatacacttg atagtgtctg tatatcatct tcaaacgtta agtatacatt ctcataaaac
    11701 ataaaaaatt ccataacttt ttctttcata ttatggatat ttctatatct attggataat
    11761 atatttatta tatcgtcagt agtatttttc ccatcacatt cagccagtat aacaatttgc
    11821 ttccaactta agctatccac atttccgata ctttttttat atccatcttc ttcatcataa
    11881 ataggaccaa ttataatgtt ttcttttggc cacagatgaa ccctagttcc atcttttaat
    11941 ttcggatatt tattttttac agactcataa aatctacttg tatcacccat gattctttac
    12001 taccccccat agtttaaata ttctatgcta aaaatagcac tttattttta ttaattcaac
    12061 aattattcaa aattggtcat tatatatgtt taaatggatt taacatcaac cttatttata
    12121 tgttattgat gtttatatat gttatataaa atcttatttc tcatacttca atgtaatctt
    12181 aactatctct ggtttacaat taagcctaat taaaactttt gagcctccta agcctttatt
    12241 taaaatcata gtgctattat ctttttcata ttttccccag tcatacttag gaaagaactc
    12301 tctatttgga gataatacac ctccaacaaa aggtattctt attatacctc catgaacatg
    12361 tcctgctaaa accaaatctg ctccccattt tgcatatttt tcaaagaaaa atggtgtatg
    12421 tgctaaaaga atgttatatt cattttcatt aactttacct agattgtctt ctacaaaatt
    12481 gtcacttagt tctaattttt tgttattagc aaataaatat ggatagcatt ccaatggtat
    12541 aattaaacca tataaattaa gatggctatt acctctatat attcttacag attcattttc
    12601 catatttatt attttctttg aatatagttc cttaaaataa tccttatata gatgtttata
    12661 cttttttatt aaagattttt gttcatgatt tccaattata tgatatactt catatttttc
    12721 aaccaaatta tctattaaat ctaatgcaac ttgaaaattt ttattttctc catctactaa
    12781 atctcctgtt ataaatataa catctgggtt taatgattca attttatcta tcaatacctt
    12841 attattttta tcaaacacct tattatgtaa atcactcact tgaactattt taaagttatc
    12901 aaattccttt ggaatttttt tgttgaaaat agtatattct gtcgtttcta tatgctgagt
    12961 ttcatactta tacagaatat ac
//
LOCUS       DAEMHZ010000120        12574 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000120 DAEMHZ010000000
VERSION     DAEMHZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.120"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..1953
                     /locus_tag="KPK72_002979"
     CDS             82..1953
                     /locus_tag="KPK72_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG2490173.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(2045..2980)
                     /locus_tag="KPK72_002980"
     CDS             complement(2045..2980)
                     /locus_tag="KPK72_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG2490174.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            3500..4012
                     /locus_tag="KPK72_002981"
     CDS             3500..4012
                     /locus_tag="KPK72_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG2490175.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLELQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(4167..5228)
                     /locus_tag="KPK72_002982"
     CDS             complement(4167..5228)
                     /locus_tag="KPK72_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG2490176.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(5352..6245)
                     /locus_tag="KPK72_002983"
     CDS             complement(5352..6245)
                     /locus_tag="KPK72_002983"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG2490177.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(6255..7736)
                     /locus_tag="KPK72_002984"
     CDS             complement(6255..7736)
                     /locus_tag="KPK72_002984"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG2490178.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLGDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(7844..8075)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8877..9785
                     /gene="trxB"
                     /locus_tag="KPK72_002985"
     CDS             8877..9785
                     /gene="trxB"
                     /locus_tag="KPK72_002985"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2490179.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(9875..11698)
                     /gene="typA"
                     /locus_tag="KPK72_002986"
     CDS             complement(9875..11698)
                     /gene="typA"
                     /locus_tag="KPK72_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG2490180.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            11910..12152
                     /locus_tag="KPK72_002987"
     CDS             11910..12152
                     /locus_tag="KPK72_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490181.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            12300..12491
                     /locus_tag="KPK72_002988"
     CDS             12300..12491
                     /locus_tag="KPK72_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG2490182.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         4591 a         1667 c         1851 g         4465 t
ORIGIN      
        1 atattatagc attaattgta gattataatg tataataatg tttgagagaa tatattttat
       61 aaaatgggta ggtagcgaat aatgaaaaat acaaaatttt ctataaaaaa aataaaaaat
      121 atatctaatg acccgaaagt cgtacatact aagggtgccc tccttgaaaa gatgggtaag
      181 atggaaagcg caatagagtt gtataagagt gctgcattgg ataattacgt aaaatctcaa
      241 tatactttag gaaatatata tgaaagtaaa aaacaattta aagaagcaga aaagtggtat
      301 tcaatggcat ataaaagtgg aagtgaagat gctgcttttg atttaggtaa tatgtactat
      361 aagttagatg gctatgaata tgctatatat tggtatgaaa agatagcaaa tttaggttat
      421 ttacaagcac aaaataattt aggtgtatgc cattttaaaa tgaaagattt tgtaaggtgt
      481 gaaaaatggt taaaaacagc agcagacaag aatttaggta aagcttgttt taatttaggg
      541 gtattatata cttttttgga gaaagatgat gaagcatatg aatactataa aaaaggttct
      601 gtgcttttag atgatgatgc aaaatataat ttagcaatat taaatggaaa aaagaaagat
      661 aataaatcaa cagtaagttt atataaacag ttgtacaagt caggtcatat gaaaggttgc
      721 tttaacttgg gtatgattat ggaaataaat gataatttag aagatgcaga gaagtattat
      781 aaaaagagtg ctgatagaga tgatgtgaag tctgagtata ggcttgcata tgtatatgat
      841 agaaaagaag agctgggaga tgcaatttat tattatgaga aagcaataga aaaaaatcat
      901 acactttcta agtatagact tgctaattta tataatagag aaaatgagat agagaaggca
      961 aaaacttatt ataaaatggc tgctgaagat gatataacag aggcaaaaaa taatctggca
     1021 ggtatatatt ttgaagaaaa agattatgaa aatgctatta aatattatga ggatgctata
     1081 gctgttggat gcaaaagttc ccttgaaaat ctaggggatt tatattatca gaatcaagat
     1141 atagaaaaag cgatttcgta ttattcgagg attccaaata atgtatcttg tcaaatcaaa
     1201 ttaggaaata tctatgaaga tttaaataat attgaagaag caataagttg gtataagaaa
     1261 gcatctgaaa atggagatac gaggtctagt tatagattgg ggtgtatata tgagagctta
     1321 ggaaatacaa aaaatgctag aaagtatttt gaaatggcaa gtagcaaaaa ccatatgaat
     1381 gctcgtatac atctaggtag aatttatttt agagaaggta aattggaaga gtctaaaatg
     1441 atgtttgata cacctgccaa tgaaaataat gtgtatgctc aacatatggt aggattaatt
     1501 tatgatatgt tctataaaga ttatgtaaat tctaaatttt ggtatgaaaa ggctagagca
     1561 caaggttgtg ttgaatcaat atataattta ggtcaaattt atttaaaatt aaatgatgac
     1621 gcagaagctg agaaatatta taaagaaggt attaaatatg gaagtaagaa gtgtgagtat
     1681 atgcttgcag gtctttatta taaaaagagc ctagatatgt acatttcttt agcaaatgaa
     1741 gagtacgata attgtgaaga gatagtagaa ggctttccaa atttaaatat agactttgat
     1801 gaagtattaa ttcctccatt taagttgcaa gaagaggtaa tagatgaaga agagtatgta
     1861 ccaatttata tactaaatat aaaagaaagt ttagattcaa tgctagaagg gcttgaaact
     1921 gaaatgatta tagatgaaga acatgatagt taaaaagtat tataggatta tatagattta
     1981 aatatataaa agccattagg ataaatgtaa atcaattaat tctaatggct tttttattaa
     2041 tattttataa tagcttattt cttgacatga aaaatgtaat cactatacat attatagagg
     2101 taataagaag tattatccaa aatccgtttg ggttattaga aaatggcatg ttgatgacat
     2161 tcataccaaa gaatcctgat attattgtag gtattgagaa aattagtgta actactgtta
     2221 aaatttgcat aacattattc tgattattac ttataattgc actgaaagca tccataattc
     2281 tactcaatat atctccatat attttagcca tatcaagagc ctgtctattt tcaactataa
     2341 catcttctaa taggtcttca tcatctggat acttctttat accagcagtt ctaaccattt
     2401 tgtttaaaac aagttcatta gattttagtg aggtagagaa gtataccaaa gatttttcta
     2461 attccaataa ttgaaccaat tctttgtttt tcattgattt gtaaatttct cgctctatta
     2521 tgattgtcat tttatttatt tttcttaaat aatgcaagta ataagctgca tttttgtgaa
     2581 gaatttgaag caaaaagcga gtctttttaa acgtaaaaaa atctttaata tgaccaacta
     2641 taaagtcatt taaaatctta gtttgtgcat cacaaactgt tacaatagct tcttcagtta
     2701 gtataatacc taagggtatt gtagtataat gagaagaatt agagtcactt tcgtctactg
     2761 gtatatctat aagtataaga gtatgattgt cttcaacatc tattcttgag cgttcttctt
     2821 catctagtgc agattctatg ctttctacat ctatatttaa taaattagaa atttcgttga
     2881 tttcactatg attaggttca acaagattta tccaacagcc atcttcaaaa aaactaagtt
     2941 tctctaattt tgaatctata gttttgtaat atcttatcat ggcaacacat cctttattca
     3001 gttttatata aatctcatca tttaatagta tctattaatt aactaattta tataactgaa
     3061 tatatgtatt agttttgatt ttagatatta ttaagtgatg aaaatgtaaa aaattctctc
     3121 cgcaacggac tagtatccat tgcccatcac tctccttatt tattatttta tatatagcta
     3181 tttatgaaat gcgtatttta agcactcttt aatataatac cttttccagg tatataaatc
     3241 aagttttgat ttgattaaat aatattttta ccataattta cacataaagt atataagtta
     3301 tatttaattt gagtaaagaa tatatatatt ctgagtgaaa ttattaaata tattaaaaaa
     3361 taagagagta tatatatatt tacttatttg caataatata tttaaaaaag tattttattt
     3421 tcaaaactag acttcattta taatttatca cgaaaataaa aggaatataa atataagaga
     3481 atgagaggtt attagagata tgagtgattt taaagaaatt ttggcagaag agattaaaaa
     3541 taatccattt ggattgatag gtaaagattg gacacttata acagcagaaa atgaaggtaa
     3601 agtgaataca atgactgcta gctggggagg gcttggagtt atgtggggaa aagatgttgc
     3661 ttttgtagta ataagaccac agcgttatac taaggaattt atagataata cagataaatt
     3721 ttctcttaca ttttttgatg aagattttcg taaagagctt tcatattgtg gaaaagtatc
     3781 tggtagagaa gaggataaga tatcacagat tggatttaat atagaacact taaatgatac
     3841 accttatttt aaggaagcta aaatggctat tatatgcaaa aaaatgtata gtcagaaact
     3901 tgaactccaa tgttttattg ctgaaggtat agatggaaga tggtatccac aaaaagatta
     3961 tcatacttta tatatagctg aaataaccaa tgtacttgta aaagaagatt agttataagg
     4021 gttaatacta tctctaatta aattaaaatt tccataaaag gagattgaca caaaaaagct
     4081 aaggacataa atatgtttat aaagattaat aaataacgta tttaaactcc ttagcttgat
     4141 aaaatcttaa tttagttttc tttaaattat aattcagaaa tcaattcatt gataacatct
     4201 tcaacagtac ttaatttatc aattttatag gcatcatttc cacaaaataa aagtgaattt
     4261 tcaacgtctc ctttaactgc attgattaga gcttgagaaa tacagtaagg agtttctttt
     4321 ggattacaag gaatcaaaca attatagcac ttctttattt ctggacgatt tattttaact
     4381 tcttctataa atttgtttct tatggcgcgt ccaggaagac ctacaggact tttaactata
     4441 tgaacatctt cttcactagc atttatgtat gccattttaa agttctcatg ggcatcacat
     4501 tcgtaagtag caacaaatct tgtaccaact tgtacaccac ttgcacccat atctatatat
     4561 tttttaacat cactactaga agttatacca ccagcagcaa ctactggtat gtttctacca
     4621 aatttttctc cataagtatt tgcaaccttt aaaatttcaa caaattcctt atcatagtct
     4681 atagaatcta tgttatcaag ttcctcgtta gagtatccaa ggtgaccacc agcttttgga
     4741 ccttcaacaa ctattaagtc agcagtagtt ttctcttttc tatcccacat ttttaaaatt
     4801 acattagcag cttttgcagt tgaaactatg ggtgctattt ttacattact tccctttact
     4861 aatgatggta gcttattagg taatccagct ccagatatta taaggtctac accagcttta
     4921 acagcttcct ttacgtatgt ttcatattct ttcatagcta ccataaaatt aataccaata
     4981 ataccaccat ttgagatttc tttggcctta gaaatatgtt tttttatagc tcttaaattt
     5041 gcatttatag tattagtttc aaaatcttcc tcatcatatc caatttgaac tccagaaatt
     5101 acaccaacac caccaagctt agcaactgca ccagctagtg atgacctaga tataccaacg
     5161 cccattccac cttgaattat aggaactttt gcaactaaat ctctaattat taactctttt
     5221 atgataatcc ctcctcaatg taaacatgtt taaagaacta gattataata cttaatatta
     5281 ttacctagtc ctaattatta tactacaaat tataataaaa gagtagttat attttgtata
     5341 gaagacgata attatattat atttaaaaca gctccattag aatcgcaaca taactctttt
     5401 atttcccaat cagattttag tttttgtagg taatttttca tattatctac aaaactatca
     5461 tcattttctt ttattaaaga cattatagta ggtccagctc cacttaaaaa tactccaata
     5521 ctatttaatt gttcagattt ttcaacaata tcattgtagt tctcaattag agttcctcta
     5581 taatcttgat gcaatttgtc ttgacaagct actttaagta ggtcaaagtt tttgtttagt
     5641 aaggcagata ttagtaaagc aactctacta acattgaata ccccatcaga atatggtatt
     5701 tcctttggta aaacacctct agctttttct gttgataact taaaattagg tatcattgca
     5761 caaaatttaa gttcttcagg aatttttata atatcataat gtatattctc attatcagta
     5821 acagatacaa tcatatttcc taaaatagca ggagcaacat tatctgggtg accttctatt
     5881 tctgaagcta tatttagtaa ttgattttta tctaaattag cacctgatag agcatttgca
     5941 gctataactc cagctactat acaagaggca ctactgccaa gacctctaca tataggtact
     6001 tcactttgga tttttatttt gataccagca ggtatttttt ctggtttaac tctatcaaaa
     6061 aagtacttca tagatgtata aactaagttg ttttcgtttt tgtaagcatc ttcacaacct
     6121 tctatttcaa gaccactttc aatttcttca acataaaatt tattgtatat atttaacgca
     6181 attccaagac aatcaaatcc tggacctata ttagcacttg ttgcaggaac tataatctct
     6241 aacataataa tcacctatac ctttaagaat ttttttatag ctagttttaa ttcatctttt
     6301 tcataaatat ctttatgtag tatttctgca tttttaagat ttttaatagg ttttggaata
     6361 tctattttag aaatacttga taatctatca attatagcaa agtcatcaag gtctttaaaa
     6421 tcacaatcta tagactttag aacatcttca gaaaacttga atggacttgc agtacttaca
     6481 ataacagttt ttgtgttatc agaagtttct tttttatatt tttcataaca attgtatgca
     6541 acagcagtat gagtatctat caaataatta taatttttaa acacattgtt tatcgtgctt
     6601 ttaacttctt cttcatttga atattctcca tagaatgagg ctaaattttt ttccatatca
     6661 tcacttattt tgtagactcc tttatcacca aggtctgata acagcttatt tacaatatca
     6721 gtatttctat ttgatatttc aaacagtaat cgttctaaat tacttgaaat taatatatcc
     6781 atagaaggag aagtagttaa tttcaatgtt ctatttttat catatacacc agttttaaag
     6841 aaatcataaa gaacattatt atcatttgat gcacatatca atttgtttat tggaagaccc
     6901 atttgtttgg cataatatcc agctagaata ttaccaaaat taccagttgg gacaacaaaa
     6961 tttattttat catttaaatt tatctcacta ttgctaacta atttcatata tgaataaaaa
     7021 tagtatgcaa cttgtggaag aagtcttcct atattaattg aatttgcaga agataatata
     7081 taattattat ccaatagttt ttgattaaat tctttatcag aaaaaatctt ctttacacct
     7141 gattgtgcat catcaaaatt tccttttata ccaactacat atgtattttt tccagtttga
     7201 gttttcattt gaagtttttg tactgggctt acaccatctt ctgggaagaa tacaattatc
     7261 tttattttat ctatatcttt aaatccctca agtgcagctt tgcctgtatc tccagatgta
     7321 gcagtaagaa tcacaacatc tttctctaaa ttatctttct ttatagatgt tttaagaaga
     7381 tgaggcatta tagtaagtgc catatcttta aatgctaaag ttggaccgtg atatagttct
     7441 aagaaataag tatcatttac tttatttaat ggagctatac tactacaatc aaacttttca
     7501 tcatatgcat tttctataca atttttaatt tcatcttcag taaaatcaca taaaaattta
     7561 ctaagtacaa aaaatgctat ctgagaatac gttaaattta ctaaatttat aagttcattt
     7621 tttacattgg gaaattcact tggaacataa agaccaccat cacttgatat tccttttatt
     7681 attgcttgtg atgcagttac tttttcttcg gtgcctctcg tacttgaata atacattttt
     7741 tataatcctc catatatttt aaatttttaa ttagctaatt aaattagata cagaatattt
     7801 taaaaactga atttaggtaa taaaaaaaga ccctcatccc taaaaaaggg acgagaatct
     7861 gtttcccgcg gttccaccca aattgatttt aaaaaatcca cttgatactt taacgcaagt
     7921 aataaaaacg tctattccta ctaaaatcat taagataact tcggatagaa gctcctaggt
     7981 agttttcaat ccttctaatt agggaaacat ttcagctagt agtttccttc tctggtaatc
     8041 gattcagatt tactcttcct aatcaatgcc tttcaatatt cttaatatta ttaattattc
     8101 tagtttataa aacagacaaa gtcaagtatt aatataaaaa tttggaaaaa atatttttta
     8161 aattgtaaaa aaattgtaat caaattgagt attagaaaaa tattttttta acttttttaa
     8221 aagtttaaaa acctagattt tcatagtgat gaagctgttt ttaatgaaag taatcaaaag
     8281 ataactatta aatatctgta atattacaat tttatactac actatattac aaaaaaagtt
     8341 tacaaaactt taatttgaaa aatgtaatta tatggtatat catataaata taagtaaata
     8401 gtgctaatat ttaaactatt aatcataatt ggttcaacaa aagtatatag ttaataatgt
     8461 ttaatttgaa gaaattgtta gtgtgaaatt acatatatat tcaaattaaa ttcataaata
     8521 tttcacataa taatagtata ctgtaaacaa aaggaggtat taaatatggg aatagtactt
     8581 ataacatcag cattagtttt ggttatgtat ggactttcaa ttctatcata tatatcagaa
     8641 agcaatatat tagaagacac tgtaaatgat aaccaataag gttttgaatt atataaataa
     8701 gtgagaactg taaataaaag ttaattttta tttgcagttt ttttatatat atgtaaaata
     8761 aagttaatta ataaaaaata atgttttgtt tatagtatat atggataata atagtaaagt
     8821 ataagaatga tgagaataat atataagaaa taataatata aagagaggta aacgttatgg
     8881 tagatatcat tgtaattggt gctggaccag caggtttaac ttcagcaatt tatgctatga
     8941 gagcagggtt aagcgttaca gtatttgaaa aaaatattta tggaggtcaa gttgcaagta
     9001 cctctgaggt agaaaattat ccagcagttc aaaagatttc tggggtagaa ttttctaata
     9061 atatatacaa tcaagcagtt gctcaaggtg ttgatattca gtttgatgag gttgaagaga
     9121 taaatttaga gggaaaagta aaggttgtaa aaacttcttc aggtgaacat aaagcaaagg
     9181 ctgttattct tgcaaatgga gttgaaagac gtaaattagg ttgtgcaggg gaacaagaat
     9241 ttacaggtag aggagtatct tactgtgcaa catgtgatgg agcctttttt aaagataaag
     9301 aagttgctat agtaggtgga ggaaatactg cactagaaga tgctttattt ttagctaata
     9361 attgtacaaa agtgtattta attcacagaa gagatagttt tagaggtgaa gaagtgttgg
     9421 agaaatctgt gaaagcaaga gagaatattg agatacttta cagtcatggt gtagaaaaaa
     9481 tagaaggaga aaagactgta tcgaaaatag aagtaaaaaa cttaaagact gaagaaaaaa
     9541 gaactataga tgttagtggt atttttattg ctataggtct taaacctaat aataaaatgt
     9601 ttgaaaatgt tcttgatttg gatgaaggtg gatatataat ttcagatgag tcttgtacaa
     9661 catcagtaga aggtgtatat gtagcaggag atagtagaac taagttttta cgtcaaataa
     9721 taacagcagc atcagatgga gctattgcag ccgttcaagc agctaattat ataaatgttg
     9781 aataatataa atttcaaata aaaatagcca tataaactga aataattttt cagtactata
     9841 tggctatttt gttaattaga ggtaaatgta cgttttattt accttgtcta gatttgttat
     9901 atcttaatct atcatgttca tttaagaatc tttttctaag tcttatagaa tcaggtgtaa
     9961 tttcaactaa ttcatcatct tctataaact ctagtgcttc ttcaagagta aatattctag
    10021 gaggagataa ttttatagca tcatctgaac cagaagcacg tacgttactc attttcttgt
    10081 ttttacaagg gttgactacc atatcatctt ttctggaatt cataccaatt atcatacctt
    10141 cgtaaacttc tacaccaggg tctacaaaca ttacagctct atcacttaag gcattaagag
    10201 aataccccat agtaacacca ggtccttgtg ctattaaaac accatttcct cttgaaggta
    10261 tttctccttt aaattcttca aatttttcaa atgaacgaac taaagtacct tctccacgag
    10321 tagcgtttat aaattcactt cgatatccta aaagaccacg agtaggtgct aagaactcta
    10381 tttttacata gtctccttct attgacatag actccatcat accttttctc atgtttaatt
    10441 catttataat agtaccagag taaacttcag gacagtttac aacaactctt tctattggtt
    10501 ccattagttt tccatcttct ttatgcatta aaacttcagg tttagaaaca ccaacttcat
    10561 aaccttcacg tctcatattt tcaagtagta tagataagtg aagctctcca cgtccagaaa
    10621 ctttgtatcc atcagtagta tcaagaggtt caactttaag accaacattt acttcaagtt
    10681 ccttttcaag tctatctttc aaatgtctag ttgtaacgaa ttttccactt tttccaacga
    10741 atggagagtc gtttactaag aagttcatag aaagagtagg ctcttctatt tttatcattt
    10801 ccataggtag tggactatct aaatcgcata tagtttcacc tatagatata tcaggaatac
    10861 cagctataac aactatttct ccactagtag cttcatcaac ttcaacttgt tttaaacctt
    10921 catacacaga aagttttgat acttttcctt tagatacaac agaatcttct ctacatatag
    10981 caacttgtgt attgttttta agagtacctt tgtaaattct acctatacca agtctaccta
    11041 cataatcatc atatgcaaga gctgatattt ggaactgtag aggttcatta tcgtaattag
    11101 gataagcttc aacgtgattt acaatagttt taaatagagg agaaaggtct tcactatcat
    11161 catccatttc aagtttagca ataccttgtt tagcaatacc atatattata ggaaattcgc
    11221 attgctcatc tgttgcattt aagtctacaa ataaatcgaa aacttcatta actacttctt
    11281 ccgctctctg gtctttttta tcaatcttgt ttataaataa tattggtttt aaaccgaact
    11341 ctaaagattt ttgtaaaacg aatctagttt gaggcattgg gccttcacta gcatcaacta
    11401 atagaataac tgtgtcaaca gttttcataa cacgttcaac ttcagaagaa aagtcactgt
    11461 gtcctggagt atcaactatg tttattttgt aatcttcata gtttattgca cagttttttg
    11521 aatatatagt tatgcctctt tctttttcaa ggtcgttact gtccataacg cagtctttaa
    11581 ctacttcgtt ctttctaaaa acaccgcttt gagaaaggaa agcatcaacc aatgttgatt
    11641 ttccagcgtc aacgtgggct ataactgcga tatttataat tttatgtttt tgtgacattt
    11701 ttgttgctcc cttcgattaa aataacttat taattatagc acaatatatt attaggtaca
    11761 aggaggaata tattaaattg tattaatata gtttaaattg gataaaactg ttagttttgc
    11821 atctgaaaat tattctgttt aaaaaaattt tttagacaaa taataacctt aatagataaa
    11881 aagattaata aaagagtaaa ggatgatata tgttggaagt aaccaaaata ataattaaat
    11941 attttgcaag tgaaagtgat acttttgtaa aagaagaagt ttgtaatttt gaagaacttc
    12001 ttaagtggtc tcataaattt attgaaggta aaaagataga agatgttttg tatataaagt
    12061 ctaaagaaaa ctataaagta aatgtgatta agtctttaga aattaaggaa ttggaaatag
    12121 tgagaaaaca acagatacca ccgtacatgt agtattaata atatataaaa ttaatatgta
    12181 atactcgcaa taggatataa atggtatata tggtaataat ttatattaaa aaaactttta
    12241 taaagtttat atttgggtat aaaagtatta attagacatt aattaggagg aacttaaata
    12301 tgataagagt atgtccaaac tgttcaaatg tggatgtaaa taagctaaaa actttggttg
    12361 gagaagaaaa tgtaaagact ggttgtatag gtcagtgtag agcatttaaa aaagaagctg
    12421 ttggaataat agacaatgag cttgaaataa aggaaacaga agcagagttt tttgaagcat
    12481 gtaaagaata aattttaaat aaaatataaa ttataataaa tagagcatct aaattgtata
    12541 agtattaaac aatttagatg ctctattttt atat
//
LOCUS       DAEMHZ010000121        12458 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000121 DAEMHZ010000000
VERSION     DAEMHZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.121"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..1211
                     /locus_tag="KPK72_002989"
     CDS             132..1211
                     /locus_tag="KPK72_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2490183.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGVKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1324..1923
                     /locus_tag="KPK72_002990"
     CDS             1324..1923
                     /locus_tag="KPK72_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490184.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(2110..2982)
                     /locus_tag="KPK72_002991"
     CDS             complement(2110..2982)
                     /locus_tag="KPK72_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2490185.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            3398..4801
                     /locus_tag="KPK72_002992"
     CDS             3398..4801
                     /locus_tag="KPK72_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490186.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            4828..6372
                     /locus_tag="KPK72_002993"
     CDS             4828..6372
                     /locus_tag="KPK72_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2490187.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            6403..7794
                     /locus_tag="KPK72_002994"
     CDS             6403..7794
                     /locus_tag="KPK72_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG2490188.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            7987..9483
                     /gene="abfD"
                     /locus_tag="KPK72_002995"
     CDS             7987..9483
                     /gene="abfD"
                     /locus_tag="KPK72_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG2490189.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSELKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            9618..9893
                     /locus_tag="KPK72_002996"
     CDS             9618..9893
                     /locus_tag="KPK72_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490190.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            9917..11224
                     /locus_tag="KPK72_002997"
     CDS             9917..11224
                     /locus_tag="KPK72_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2490191.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            11244..12362
                     /locus_tag="KPK72_002998"
     CDS             11244..12362
                     /locus_tag="KPK72_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2490192.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         4779 a         1378 c         2404 g         3897 t
ORIGIN      
        1 ctaatgaagt attaaaatga aattgataaa atatgaaaaa attttaggaa taaagtatct
       61 gttagataaa aataagatta attagtgata taatataatt aagataaaat ttattatttg
      121 gaggtaaaat catgagcaga gtaaaaaatg tagtagaatt gttggaaact aaaggtgttg
      181 atgcactata tctaacaaag aaaactaatg taaactatat aagtggtttt cctgatgaag
      241 aagcatatgc agtgatttgc aaagatggaa actttttagt tactgatagt agatatatgg
      301 agcttgcaga aaaggtttgc aaagattttg aaattataaa ttggcataat tttgatagaa
      361 gtgtggcaaa ggcagttaaa agtgtatgtg ataaagtggg tataaagaaa cttgggtttg
      421 aaagaactaa tatagtgttt gataaatatg aagaacttaa aaatctgatt gagaaggata
      481 atggagaatt aatacctaca gaaaatattg tagagacttt gagatatgta aaagacaaag
      541 atgaaatcaa aaatacaaga aaagcttgtg aaatagcgga taaggcatta gaagaattga
      601 ttccacatat aaaagctgga gttagcgaaa ttgaactggc tacaaggtta gagtacttta
      661 tgaaaatgaa cggagcacaa aatatagggt ttgaaactat actgatatca ggagtaaaga
      721 cttcattgct tcatggtaag cctagtgata agattataga aaaaggtgat tttgtactta
      781 tagactatgg tgctatgtat aatggctata tatctgatac aactagaact tttatagttg
      841 gaggagcttc tgaaaagcaa ttagaaatat ataatttagt taaggaagct caaaatgtag
      901 gggttgagaa catgaaagct ggtgtacatg cgacaattcc agatgctgag ataagaaaag
      961 ttgtcaaaaa atatgaagac tattactatc aaggtatagg acatggagtc ggtagagatg
     1021 ttcatgaaga acctttcatt ggtaattatg gtgataaaat catagaagag ggatgtataa
     1081 taacaatgga accaggtatt tatttccctg gatggggtgg agttagaata gaagatactg
     1141 ttttgattac taaaaatggt ccagaaagac ttactaaatt ccctaaagat ttgatgattt
     1201 tagacaaata atttaattta tatatatttt tttacataaa atataacata atctcactat
     1261 taagctctat ttacatatta tagtaaagag ggcttaattt tattgtgaga ggtgatagta
     1321 tatatgtata aaaaacataa atttataaaa aatataaaaa aagcctggta taaaattaaa
     1381 ttaattgata gatgccttat aattgtaatg gcaatattaa tgtttcaatc tatatataat
     1441 ttatttgtta atgaagtaaa ttcacaagat actacaacta ttgatgtagt agtaagaacc
     1501 acttcagcag ctatatttgg gtatttttta agtgctaatt ttataaagag acctttaagg
     1561 aagtatacaa atactgatgt atcttcaaat ctttttatag ataataattt atccccaaaa
     1621 ggtcaagagt ctaatcaaaa taatataatg aatgtcaaaa ataccattgg atttacttca
     1681 gaagctgata attatgaaaa gaaaatacct attaataata atgaaggttt tgaagaagga
     1741 gaaactagtg aattgcaaat tattatagct actgtaattt gtatagtagc attaacagta
     1801 ctttttgttg taagaaattt tacaactaca acatcagctt ctttaggtac tatttcacag
     1861 atgagagatt ttgtctcagg atgcgtgggt tttttgctag gttgtccaag taaacctgat
     1921 taaaaaagta ttatcaataa aaggtatttt aaaaagtatc agtataataa tatttagatt
     1981 taaatgtata caattataat tctataacat tctatattgt atttttatac tgatactcta
     2041 aactatacta tttattaaaa tattttaaca ttgtatttaa acaaaatatt atatctgtat
     2101 aatataaatt tagtttatat gaatagatgc catatactca ataaaaagtt tcatactatg
     2161 agatatgtat tgatttcttc tccaaagaac cccaatattt gcagtaattg cacgttctaa
     2221 tggtattgaa cagagatttt ctgtattttg aaatatttct ttatacaata ttgttgaagc
     2281 caagtcattt tctagtaaat aacgtatggt agaaagttga tttgagtgta aaattatatt
     2341 tggcttagct ttttcaagtc tttttgtaat aatcttatag tgataagaac cttcagataa
     2401 gattacaaaa ggtacatttt ttaacatata attcgatata ataggatgtt ttgccaattc
     2461 atttttggga tttgtaacta agtataattc tgttttatag attgtctttg aatctatatc
     2521 atcataaata tcactgtcaa ttataccaat acaaaaatca agtgtagatt cttcaatcat
     2581 tttagctcca acaaatgttg gaacttcaaa aattttaagc tctatatctg gataaattac
     2641 attaaatcta ggaagtattt ttgaaagcaa aaacgtacct aaaactgtag gaatacctat
     2701 tttgagagaa gcttttctct tttttcctaa atcaagtgcg tcacattgaa aatcctcaaa
     2761 ttgttttata aaatgttttg acttgtctaa aaaaattttt ccttcaggcg ttaactctat
     2821 tttattacct attctgtaaa aaagttcaaa gccaagttct ttttctaatt ctttaattgc
     2881 taatgttatg gatggttgag aaatatgaat actttccgca gccttagtca tgtttttata
     2941 tttacagaca gtttgaaaat agtataaata gtttatattc atttggtatt agttcctttc
     3001 atttacatat tagtgacaag tatataatta acaatagcat aagttttgta tattgttgta
     3061 aaaaaataga atagttttaa actatcatag cataagtttt tgatattttc aatctattaa
     3121 gaatagtgct attattaaat taacaaaaga gaatagctgg tatggtttag agtaaatcaa
     3181 tctatattac agttatatca acgaatatca aaaaattatc tattaaaaaa taaaaattat
     3241 ttttacaatt aaattgttaa ttaaatcata aagaaaaaag tgatattaac aagataaaat
     3301 ttttttaatc agaaatgtta atttaataac aaaatagagt attggggaga aaggagaaat
     3361 atatttttta ataatatttg ttaatttaat aacaaaaatg aaagggagtg atttagtgaa
     3421 gtttgaattg atggattttt taatgaatcc ttttgtcctt atgtttgctg cagtaattac
     3481 aggaatttta tttggaaaaa ttaaatttgg gaagtttaat tttggggtat caggagcatt
     3541 atttacagga ttatttattg gatggcttgc atatagcttg gggaatctaa taatagcaaa
     3601 aggggaaaca gcagcaggat ataaggcagc aactgtaatg atggggaatg gaattatttc
     3661 ttctgacttc tttgatttct ttttaataat atttgttgca gcagttggtc tattggcagc
     3721 aaaagatatg aaagctgtat taaagaagta tggagcaaga tttgtaattt taggagttct
     3781 tataactttt ataggtggct ctatgactta tgcaatgact ttactatcaa gtgataaagg
     3841 aagtagtgct tatgaagttt ctggcgttta cacaggagca ttaacaagtt cgcctggatt
     3901 agctgctgct ttagaaacag ctggtaaaca tgcagaagat gtatcaaaag aatttgaaaa
     3961 agcttcaata aaagataaaa aagaaatttt aaaagtagtc gacccagaag gcaaattgga
     4021 tgtgaataca actacatcac tgactcaaga gcaaatagat aagtatattg catatgctga
     4081 agctggtgtt ggtataggtc atgcagtcgc atatcctttt ggagttttaa tagtaatctt
     4141 aggtgtaaat ttccttccaa aattatttag gatggaccta aaagaagaaa gaagaaaata
     4201 tgagaaggaa atgaaggaag caagagatag tgtttctggt aaaacgatac cagaagttcc
     4261 atttaatata ataacatttt tcttgacatg tttagctgga tacttagttg gaggaataca
     4321 tgtatttatg ggaccactag ggtacttcac actaggtgca acaggaggtt cactaattgt
     4381 atctctagtt cttggatata taggtaagat aggggtagta aatttcagaa tggaagaaaa
     4441 ggttttaaat attttaaaac aaatagggct agtatttttc ttagcaatag ttggattaag
     4501 atatggtgga aaagtagttg attcaattat gacatctggt atgcacctag ccttagtagc
     4561 aatagcagta ggtgttacag ctatgatgat aggattctta gttggcaaat acgtatttaa
     4621 attaaattgg attttattat ctggagctgt ctgtggtggt atgacatcaa caccaggtct
     4681 tggagctgct gttgatgctc ttgatagtga tgacccagca gcaggttatg gtgctacgta
     4741 tccatttgca ttattaacta aagttatatt ggttatagtt ttacacaaac ttccaatgta
     4801 aataagattt tatatggggg gatatttatg aataatgaag gagcatttga agaattaagt
     4861 ggaagattaa gaaataaaga attgtgtgat ttaataatga atgcagatga agctgctaaa
     4921 attatagagg atggcatggt tattggagtt agtggattta caccttcagg ctatccaaaa
     4981 gcagtaccat tagcagtttc agaaagagct aaaagtggag aaaagataaa attaaccgta
     5041 tattcaggag catctttagg tccagaagtt gatggagcat ggtcagaagc aggtataatt
     5101 gaaagaagac ttccttatca aactaattca atacttagaa ataatattaa taaaggtgtt
     5161 gttgattata tagatatgca cctaagtcat tctactcaat ttttaaatta tggaactata
     5221 cctaaagtag atgtagctat agtagaagca cttgcaataa ctgaagaagg gaatattata
     5281 ccaactagtg ggataggtaa ttctccatct tttataaaaa gtgcagataa agtaatagta
     5341 gagataaatc ttgcaaaacc aatggaaatg gaaggtatgg cagatatata tattacggaa
     5401 aatccaccaa atagaaaacc aatagaaata aatcatccta aggatagaat tggaacaaca
     5461 tatatacctt gtggtttgga taaaattgca ggagtagtaa ttaccaatat gcaagataag
     5521 acccgtccat tgggggttgt agatgaagct tcgaaaaaaa tatcaaataa tattattgct
     5581 tttttaagag aagaagtaaa ggcaggcaga ttatcaaaga atttacttcc tcttcaatct
     5641 ggtgtaggaa gtgtagcaaa tgcagtatta tatggattat gtgaatctga atttgaaaat
     5701 ttaacttgtt atacagaggt tgtacaggac tctatgttgg atttaataag aatgggtaaa
     5761 gttacaatgg catctactac atctgtaagt ccatctccag aaggattaat aaaatttgaa
     5821 aaagatattg atttttttaa ggataagatt atattaagac ctcaagaaat tagtaacaat
     5881 ccagaaatag ccagaagaat tggtgtaatt gcaatgaaca ctgcaattga agttgatatt
     5941 tatggaaatg taaattctac tcacataatg gggtcaaaga tgatgaatgg aattggtggt
     6001 tctggtgatt ttgctagaaa tggagcaata actatattta gtactgagtc aattgcaaaa
     6061 aatggtgata tatcatcaat agtacctatg gtatctcacg tagaccacac tgaacatgat
     6121 gtaatggtaa ttgtaacaga acaaggatat gctgatttaa gaggtcttgc acctagagaa
     6181 agagctataa aaataataga aaattgtgcc catccagatt ataaagaaca acttagggat
     6241 tatttaaata gagcttgtaa aagtggagca aaacaaacac ctcacatatt ggacgaagct
     6301 ttatcatggc atagtaaatt tatgagtaca ggcacaatga aaaaggctga aacttttaaa
     6361 agtgcattat aaaatattta ataaattgga ggtagatttt aaatggaaaa agcagtggaa
     6421 aactttgaag atttaagtaa ggaatacatt aatggttata ttgagagagc aagaaaagct
     6481 caaagagagt ttgaatgtta tactcaagaa caagtagata aaatagtaaa aatagttgga
     6541 aaagtagtgt attataatgc tgaatattta gctaaacttg ctgtagaaga gactggaatg
     6601 ggagtatatg aagataaggt agctaaaaat aaaagcaaag caaaagttat atataacaat
     6661 ttaaaagata aaaaatcagt tggtataatt gatatagata gagaaactgg aataacaaag
     6721 gtagctaaac cagttggagt agtagcggca ataactcctt gtacaaatcc aatcgttaca
     6781 cctatgagca atgcaatgtt tgcacttaag ggaagaaatg caattattat cacacctcat
     6841 cataaagcaa ttggatgtag tacaaaaact gtagagatga ttaatgaaga attagaaaaa
     6901 ataggagctc cagaaaattt aatacaaata cttgaccaac aatcaagaga aaatacaaga
     6961 aatttaattt catcagcaga tgtagtaatt gccactggag gaatgggaat ggtaaaagct
     7021 gcgtactcaa gtggaaaacc agcacttggt gtaggggcag gaaatgttca atgtatcata
     7081 gatagagatg tagacatcaa agaagcagta cctaaaatca tagcaggaag aatttttgat
     7141 aatggtatca tttgttcagg tgaacagtca gtaatagtag cagaagaaat gtttgataaa
     7201 ataatggatg aatttaaaaa taacaaaggt tttatagtta gagataaagt acaaaaagaa
     7261 gctttcagaa atgctatgtt tgtaaataaa tctatgaata aagatgcagt aggtcaatca
     7321 gttcatacaa ttgcaaaaat tgcaggtgtt gagataccag aagatacaaa aataatagta
     7381 atagaagctg atggaccagg agaagaggat attattgcaa aagaaaaaat gtgtccagtt
     7441 atatctgcat acaagtataa aagttttgaa gaaggtgtag ctattgcaaa agcaaattta
     7501 aatgtagaag gaaaaggaca tagtgtatct atacattcaa atacagttaa aaatatagaa
     7561 tatgcaggag aaaatataga agtatcaaga tttgttatta atcaatgttg tgctactagt
     7621 gctggaggaa gtttctttaa tggtcttgca cctactaata ctctaggttg tggttcttgg
     7681 ggaaataaca gcatctctga aaatcttgat tataagcatc taattaatat ttctagaatt
     7741 gcttattata tgccagaaaa tgaagtccct acagatgaag aactttgggg ataaaattta
     7801 gacaaaaaat attcattcta aattaaagta ggaatatatt ttaatttaga atgaatttaa
     7861 gacataaatt aaatttaaat tagtttattg gactaaattt aaaatattaa gagacatata
     7921 gaagaaggaa gtattataat ttaaaataaa taaaaaaatc tataaaagag aaggagtggt
     7981 tttattatgg cattaatgac aggagctcaa tatattgaaa gtttgagaaa attaaatact
     8041 aaggtttata tgtttggtga agaggttaag aattgggttg accatcctat gattagacca
     8101 tctataaatt gtgtagcagc aacttatgac ttagctcatg acccagaata tgcagattta
     8161 atgacagtta cttcaaatat cacaggtgaa aaaataaata gatttggtca tcttcatcaa
     8221 agtgttgatg atttgataaa aaaagtaaaa atgcaaagac tatgtggtca aaaaacagct
     8281 tcttgtttcc aaagatgtgt tggtatggat gcgtttaatg cagtgtattc aacaacattt
     8341 gagtgtgata aagcccatgg aactaattat catgataatt ttgtaaaata cttaacttat
     8401 atacaagaaa acgatttagt agttgatgga gctatgactg accctaaggg agatagaagt
     8461 ctatcaccta gtgctcaacc tgacccagat atgttccttc atatagtaga aagaagagaa
     8521 gatgggataa ttgtaagagg ggcaaaggca catcaaactg gttcaataaa ttcacatgag
     8581 caccttataa tgcctactat aagtatgact gaagcagata aagactacgc tgtatcattt
     8641 gcagttcctt cagatgctga aggagtgttt atgatttatg gaagacaatc ttgtgacact
     8701 agaaaattag aagaaggtgc agatgttgat ttaggaaata aggagtttgg aggtcaagaa
     8761 gctttagtag tatttgataa tgtatttgtt ccaaatgata gaattttctt atgtggagaa
     8821 tgggatttct ctggtatgtt agtagagaga tttgcaggat accatagaca aagttatggt
     8881 ggatgtaaag taggagtagg tgatgtaata ataggagcag cagctttagc agctgattat
     8941 aatggagcaa acaaagcttc acatataaaa gataaactaa tagaaatgac tcaccttaat
     9001 gaaagcttat attgctgtgg tatagcatgt tcatcagaag gtcacaaaac agaagctgga
     9061 aactatcaaa tagatttact tcttgcaaat gtgtgtaaac aaaatgtaac tagatttcct
     9121 tatgaaatag taagacttgc agaagatata gctggagggc ttatggttac tatgccttca
     9181 gaaaaagact ttaaatcaga acttaaagtt ggaacaagtg gaatgactat tggcgaagta
     9241 tgtaacaagt attttaaagc ttcttcagtg gcttcaacag aagaaagaat gagaatatta
     9301 agattcttag aaaatatatg ccttggttca tctgctgttg gttatagaac tgagtcaatg
     9361 catggggcag gttcaccaca agctcaaaga ataatgattt caagacaagg aaacataaat
     9421 caaaagaaag aattagctaa gaaaattgcg ggaataaaaa aagaggaagc tttaaattta
     9481 taaaaagttg atatataaag ggaaattata tattaatgtg tattaattaa tgaaaaaata
     9541 tgtagtataa gagatagtta taaaattatg gtggttttta gctaagtaaa gaaattttta
     9601 ataaggagaa tttacacatg acctgtggag ttagattttg tgggggctgt aaccctagat
     9661 acgaaagagg gaaggctctt acaataatca aagagcactt caatggtagg gtagatttta
     9721 gtatagcaca agagaacgtc gagtatgatt tgctacttat aattggagga tgcgcaactc
     9781 tatgcgcatc ctatcaacaa tataaagtga aaaatgaatg tgttaagatg tgggatttta
     9841 gccatataga aagagttata aatgtgattg aatcagcaat ttgtgaaact taaaaaagct
     9901 aaatgggggg atttcattga gttggcaaga attgtatcaa agtaaattat gtagtgctac
     9961 agaagctgta aaacagatta aaaacggtga tactgtagta tttgcacatt gtgtaggtga
    10021 acctcctgca ctagtagagg ctatgattga aaatgctgaa caatataaag atgttgagat
    10081 taaacatatg gtaagcttgg gtagtggtgg atatactgct aagggaatgg aagctcattt
    10141 tagagtaaat ccaatgtttg taagtggaaa tgtaagaaag gcaattgaaa atggagatgg
    10201 tgattttaca cctgcattct tccatgaagt accaaagttg cttagagaaa aaagattaaa
    10261 atgtgatgtt gttttagcac aggtaacccc accagatgaa catgggtatt gtagcttagg
    10321 aacatcagtt gattatactt atgaagccat taaaactgct agaacagtaa ttgttcaagt
    10381 gaatgaccag tttcctagaa cctatggtga ggtagtccat gtatcagagt ttgactatat
    10441 agtggaaaaa tcacaacctt tatttgaact acaacctgca aagattggag aagtagaaga
    10501 agcaattggt aaaaattgtg cttcattaat tgaagatggt tctactttac agctagggat
    10561 tggtgggata ccagatgcag ttatgttatt tttaactgat aaaaaggatt tagggattca
    10621 ttcagaaatg atttcagatg ggactttggc cctttatgaa aaaggtgtta taaatggtaa
    10681 atataaaaat tttgataaag aaaaaatgac agttaccttc ttaatgggta ctaaaaaatt
    10741 atatgacttt gcaaataata accctgcagt agaggtaaaa ccagtagact atgtgaatca
    10801 tccagcaatt ataatgaaac aacataagat ggtttctata aattctgcta tacaagttga
    10861 tttaatggga caggtagttg cagaggctat gggacttaga caatttagtg gtgttggagg
    10921 tcaagttgac tttatacgtg gagtgtctat gggtgaagat ggaaaggcaa ttatagctat
    10981 gccttcaata actacaaaaa aagatggtac agtaatttca aaaatcgtat ctattgtgga
    11041 tgaaggtgca cctataacga catcaagaaa tgatgttgac tatattgtaa cagaatacgg
    11101 tatagcagaa ttaaaaggaa aatctcttag agaaagagca agaaatttaa taaatatagc
    11161 tcatccaagt gtaagagaac atctagcagt agaatttgaa aagagattta aggagaaata
    11221 ttaataatag aggggtgtta ataatggaag cacttagagt agttccaaag atattttact
    11281 ttgatacatt taaagaattt aatgaagaat ttaaaatagg aaaaaatgat ttagtaatta
    11341 ctaatgagtt tatatatgag ccatatatga aacctcttgg aatagataca aatttaattt
    11401 ttcaagaaaa gtttggaaca ggtgaaccat ctgatgaaat gatagactcc atgacaaagg
    11461 aaatgaagaa atacaatttt gatagaatta ttgcatttgg aggaggaaca atagttgata
    11521 tatgtaagat attagcactt gatgttccag aaaagtctat tgatttattt gaaggagaag
    11581 tatcacctaa aaaagtaaaa gagttagtag tagttcctac aacatgtggt acaggtagtg
    11641 aagtaacaaa tgtagctatt gcagagttaa aatctaaaca tactaaaaag gggcttgcag
    11701 tagaagaaac atatgcagac tatgcagtgt taattccaga gacagtaaaa gggttacctt
    11761 acaaattctt tgttacaagt tcggtagatg ctcttataca tgctattgag tcttatcttt
    11821 ctccaaaagc aagtccattt acagagatgt attctctaca agcaataaaa atgattatgg
    11881 atgggtataa gaagatagtt gataaaggag aagaagagag atttaatcat cttagagact
    11941 ttgtacttgc atcaaattat gcaggaattg cttttggaaa tgctggttgt gcagcagttc
    12001 atgcactatc ttattcaata ggtggagctt tccatgttgc acatggtgaa gcaaattatc
    12061 agttctttac tgaagtattt aagatgtatt ctagaaaaaa acctaatgga aaaattataa
    12121 aatgtacaaa aattttagca gatgcccttg aatgtgaccc taactgtgat gtttatggag
    12181 aattagaaaa gtttttaaat aaattaatag ctaaaaaagc tttgagagaa tatggaatgg
    12241 ttgaaagtca gatagatgaa tttacagata gtacaatagc aaatcaacaa cgactacttg
    12301 ctaataatta tgtagaactt agtagagagg aaataagaga gatatttgca aatctttatt
    12361 aatacaattt tttatttata tgtatagtca acataaacaa tgctaattat atttatgtac
    12421 aaataaaaat aagggaactt aaattttaat taaagtta
//
LOCUS       DAEMHZ010000122        12231 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000122 DAEMHZ010000000
VERSION     DAEMHZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.122"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..257)
                     /locus_tag="KPK72_002999"
     CDS             complement(<1..257)
                     /locus_tag="KPK72_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG2490193.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFY"
     gene            484..1185
                     /locus_tag="KPK72_003000"
     CDS             484..1185
                     /locus_tag="KPK72_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490194.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1267..1875
                     /locus_tag="KPK72_003001"
     CDS             1267..1875
                     /locus_tag="KPK72_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2490195.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLIAQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            2199..2663
                     /locus_tag="KPK72_003002"
     CDS             2199..2663
                     /locus_tag="KPK72_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBG2490196.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFRVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            3300..4316
                     /gene="gap"
                     /locus_tag="KPK72_003003"
     CDS             3300..4316
                     /gene="gap"
                     /locus_tag="KPK72_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2490197.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            4711..5880
                     /locus_tag="KPK72_003004"
     CDS             4711..5880
                     /locus_tag="KPK72_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490198.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            6260..10513
                     /locus_tag="KPK72_003005"
     CDS             6260..10513
                     /locus_tag="KPK72_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2490199.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            10662..11156
                     /locus_tag="KPK72_003006"
     CDS             10662..11156
                     /locus_tag="KPK72_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2490200.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            11272..11649
                     /locus_tag="KPK72_003007"
     CDS             11272..11649
                     /locus_tag="KPK72_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG2490201.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            11855..>12231
                     /locus_tag="KPK72_003008"
     CDS             11855..>12231
                     /locus_tag="KPK72_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG2490202.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISI"
BASE COUNT         4992 a         1207 c         2151 g         3881 t
ORIGIN      
        1 atataaaaat atactaataa aaattgtaaa aatagtataa atggtatacc aaatgtaaat
       61 ttaatgtgtt tagttttatg tctaaaagaa tacattccaa ttatagaacc tatactacct
      121 ccaattactg ctatagacat aagagtagct tcttttattc tccattcgtt tctaattgct
      181 cttttcttat ctatgtacat tgaaaagaaa gcaatagaat taatcactat aaaatatatt
      241 agaataaaat ttttcacagt cttgcctcca taaaaatata ttatacatta taaaacaaga
      301 tatttgtttt ggaaagttaa atttgtaatt agtacatata aaatatttga taagaacaaa
      361 tataatatta attggaaaat ttaaaaaaat taaacaatta gcaaataggg ggtatattta
      421 taagacacaa agtgataaaa tctatttaga aaataatgta aaataacagg aggataaagc
      481 aaaatgaaat ttatagtaga aaaagaagtt tttgataaat tagaaaatgt atgctttggt
      541 gtagtagttg caaaaggaat agataatact aaagaaatag aaagaataag caacctttta
      601 gatataagta tagatagagt agaagattac ttcaaagata aaaaagtaaa ggaatcagaa
      661 gaaattatac catatagaga ggcatttaga agtctaggca tgaacccaaa taaatttatg
      721 agttcgatag aagcaatgac aacaagggtt gctaagaata agaaacttcc tcatataaat
      781 cctattgtag atttaggaaa ctctatttct ttaaaatatt tacttccaat gggtgctcat
      841 gatatggatt ttagaaatga tgatgtatat gttagatttt ctaaaaaagg ggataaattt
      901 gtaccatttg gagaaactga tgttgaatta atggaagaag gagaattaat ttattcagtt
      961 ggagacatgg taaaaactag aagatggata tggagacaag gagaagaagg aaaaattact
     1021 aatagctcaa aaaatatatt ttttcctata gatggtttta ctgatgctaa tttagataag
     1081 gtaatgagtg caagagaaga attagcaaaa ttgttaaagg aaatatttaa ttgtgaaatt
     1141 aaagtgggtt ttgtagataa agataatcct gaaatggaaa tataataagt tgttgaatac
     1201 ttgttaagat attatattta cttaataatg ttaaaaaggt attaaaaaag aaaggtcgta
     1261 gtaaatttga aaaaaactat aataaagaat gtaaatccaa tgattgaaaa taagtttata
     1321 ggactttttg aaatagagta taaaaacaaa ttagatgaag ataaggtttg gatggtagca
     1381 tcaagaaaaa gtagtgagca attaaaaaat atttatttga agaatgaaga agatagtgta
     1441 gatgcagttg taatagttgg gcttcataaa agtagtaaaa aattaatttt aataagacaa
     1501 tttagagtac ctattaatgg atatatatac gagttaccag caggtcttgt tgatgaagga
     1561 gagtctatag atatttctgt tgaaagagaa cttagagaag aaacaggact tactctttta
     1621 gaaattaaaa aaaataaaag tattgataaa gtttatttat ctcctggaat gagtgatgaa
     1681 tctgttgcct ttgtttattg tatatgtgat ggtgatataa cagatgaatt tttagaacca
     1741 gatgaagaga ttgaagcgct tttaattgcg caagaagatg caaaagagat tttacaaagt
     1801 aatcataaga tagacacaaa agcatttttg attttacaaa tgtttgtgag tttaggagct
     1861 aaattatttg aatagatata tagaaaagag gttatataga agagtaaaat aataaagttt
     1921 attttacttg tataaattat aatctctttt tttatacaat aaagtataat tttgtgtatt
     1981 tttaaataag tgacttgact cttaaatttt cataatatgt tataaaccaa tttaagatac
     2041 tacaataatt aaaattccaa aattatttaa catatttgca attaaaatga gaaaaggtaa
     2101 aggctgaatg attgaaaaat ttgatgtaaa atagtagatt agtttaatac tatagtttgt
     2161 agggttaaaa attagaaatt gtaagggaga taaattctat gaaaaaatta gttattattt
     2221 taattagtat cattagtgtg gttagcttga cagcttgtaa aaaaagcaat gaaagtaaat
     2281 ataataattt aaacaacaaa gaatcaggac aaacaaccat tgaaatggac ttagataaaa
     2341 attatgatac ttcagaccca tttgttaatg cacgtttgtt tcgtgtgtct aatgatatag
     2401 atgttttaga cacagagatt tcttttaaaa tggatggaga tagtggtatt gtggaaataa
     2461 aggacaataa aacagatgaa actttatgga gtaatacatg gcatggaagc gttgataatg
     2521 atacattcac aatttctctt tctaatatac aaaaagagaa agagtatgct atatgcttta
     2581 ctggaattaa aataaatcat gcagttgtaa aggttacttt tgaaagcaat ttagttaagg
     2641 agaaagaaaa accctcaaaa taaattccag cttttctata acttgatatt cagtacataa
     2701 aagcagttga tatttatgat tgactgcttt ttcttatcaa aaaggatagg ttgtgcattt
     2761 ttacttgaaa tagtttatgc acatcaaaag ttggtgagca tgatattgga aatggtaagc
     2821 atgaaatctt gataatacaa ggaaataaat ttaagttttc aattaagtat atttccataa
     2881 tttagttaac aataagctaa ttattattta tattttgata tataaataat aattgtagca
     2941 aattttaaaa aatcatatat tttttgaact aaataaaaat ttatttttat tattttaaat
     3001 tagaggagat tcttgaagat atattatttt taaaaataag tatagtttaa aaattcaaaa
     3061 aaaagatggc aaaatatgtc ccaagggaca ttgagtaaaa taaatgattt cagcttattt
     3121 acactaaaaa gtacatacta aatgaggggt aaaataaaga aaaatgatat actaattgtc
     3181 ttttgataat aaaaaaacat ggaatttgat tatttaatca agaaaaagta ttgaacaaat
     3241 gatttattta gtgtacaata atcatgtaaa aacatagtaa tggagggaga catttcataa
     3301 tgaaaattaa ggtaggaatg aacggatttg gaagaatagg aagagctgta ttaagaatag
     3361 ctcaagaaga attaggagat aatatagaaa ttgtagccat aaatgcaaga gcaacaacag
     3421 aatctttggc acatttattt acatatgatt catgctatgg aacttttaga ggtgaagtag
     3481 aggcaaagga tgaagacact ctagtagtaa actctaaaga gataaaaata ttaagatata
     3541 atgacccaga agaattacct tggaaagaat tgggggttga tatagtaata gaatcaacag
     3601 gattatttac acaaagagaa aaggcagaaa aacatattaa agcaggagca aaaaaagtaa
     3661 ttataacagc tccaggaaaa aatgaagata taactatagt tatcggagtt aatgagaaag
     3721 aatatgataa tgaaaagcac aatataattt caaatgcatc ttgtactaca aattgtctag
     3781 ctccttttgc taaagtattg gacgaaaaat ttggtatagt aaaagggtta atgactacag
     3841 tacattcata tactaacgat caaagaatat tagataaaag ccataaagat ttgagaagag
     3901 caagagcagc ggctgaatct ataataccta ccactacagg agcagcaaaa gctgtgtcaa
     3961 gagtactacc acaattagaa ggaaaattaa atggtttttc acttagagta ccaacaccaa
     4021 cagtttcact tgttgattta gtgtgcgaac ttaaagaaaa cgttacatct gagcaagtta
     4081 actctgtgtt aaaagatgct gctgaaggag aattaaaagg tgttttagga tattgtgaca
     4141 agccacttgt ttctatagat tataaaggag attcaagatc atctattata gacgctctat
     4201 ccacaatggt tatagaagac aatatggtta aggtagtttc ttggtatgat aatgaatggg
     4261 gatattcttc aagaactgtt gacttagtaa aatatgtagc tgaaaaatta aaataatata
     4321 tatttcttaa agcaaatttt aaaaaaattt tttaaaaaaa ttatataaaa ctaaaaaaaa
     4381 agattatgaa aaatattgaa ggaaaaattt tttcataatc ttttttcttt catgcaaaaa
     4441 gatacaagtt acaacttata tttgcatatt aggtcgttag agaagtatat atatatttag
     4501 taaggaatca tactttttat aaaatgaaag ataagatgtc gaaaacttgc ataaatctga
     4561 aaaattttta ttattaaaat actgatttcc attttaaaaa tgataaaaaa atgacaaata
     4621 aagttgcaaa tagtaatact tataagtata attaaaaaag agaaaaggtt ttgttaaact
     4681 ttataacaaa aaaagaaggg atgattcaaa atggagaagt taacagacag gagacaggtt
     4741 aaggcagtaa gcatggaaac cttcattttt ctaatattac tagtagttgg ttttgggtat
     4801 gttggtagta ttatgggagc aggaatgatg ttcaaagtaa taatgagtac agcacatgct
     4861 ttactattgg agacagtttt cttaattatg gctatggcag ttcttgcagg cgcattaagc
     4921 gcactacttt cagagtttgg agtaatagcg ttaattaata aaattttcgc tgtatttatg
     4981 aagcctttat atggactacc tggtgctagt atagcaggag ctataacaac ttatttatca
     5041 gataatccag ctattatacc ttttgctaaa gataaaacat tcacacagta ttttaaacaa
     5101 taccaagttc cagcattatg taacttagga acagcctttg gtatgggact tatcttaaca
     5161 acatttatga tttctcaagg tacagaatat gtacttccag cattaattgg taatttaggt
     5221 gcaattatag gtagtgtcat aagtgttaga ataatgttga catttactaa gaagttttat
     5281 aaatacaatc cggaagaaga taaagcaaca ggaacattag agaaaaagga tgaatttaga
     5341 gaaatcagag aaggaaatgt atttcaaaga gctttagatg caattcttga aggtggtaaa
     5401 atgggggtag atatgggaat ggctataatt cctggagtcc tagtagtatg tacattggtg
     5461 atgttattaa cttttggacc atctacagat cctgttacag gtcaagaagt ttatacagga
     5521 gctgcatatg aaggaataaa attacttcca gttattggag ataaacttgg atttatatta
     5581 gaaccattat ttggatttac atcaccagag gcaatagcat tccctataac ttcattggga
     5641 gctgttggcg cagcaatgtc tctagttcca gagtttataa agtcaggagc tataactcct
     5701 aatgatatag cagtatttac agctatgggt atgtgttgga gtggatattt aagtacacat
     5761 attggtatga tggatgcatt aaatgctaga cagttggcag gtaaagctat tctttctcat
     5821 acaataggtg gtttatgtgc aggagcagct gctcacttta tatttacact cgtaggataa
     5881 atgtaatcag ctaagataaa aaataatata caaaaaatgg ttgttgatat gtaataagaa
     5941 tttacatatc aacaaccatt tttttctgac taaagataat ttataataca taaacattaa
     6001 gtggatatta gagttaagtt atatttatgt aaaaagtaac aaaaatctgt agaaatttat
     6061 gacaaaattt agtacactaa ctatatgttc ttatttaaaa tttaaggtta gaattaggaa
     6121 ttacctagta gagtaagctt tgattaataa aagaatcata aaataaaaat attagtcata
     6181 aagattataa ataacttact taaaataaag ccatagaact tacaaaaatt aaaaaagcat
     6241 ataaatatgg aggggtacaa tggatgaatt atttcatata ggaattgatg taggttctac
     6301 aacggtgaag gttgtggtac ttaataatag taataatata gttcataaag aatatagaag
     6361 acattattct gatgtaaaaa aatcagttaa agaagtacta aatggtatat atgaaaaatt
     6421 gggagatata aatactacca ttataataac tggttcaggt ggaattggaa tatcaaaaaa
     6481 attaggtgta aaatttgttc aggaagttat atctagtaca aaatcaatag agtattttca
     6541 tcctgaaact gatgttgtca tagaacttgg gggtgaagat gcaaagataa catatctaag
     6601 tggaggaata gaccaaagaa tgaatggaat atgtgcaggt ggcactggag cattcataga
     6661 ccagatggca tcacttttaa agacagatgc cagtggatta aatgaacttg ctaaagggta
     6721 taatgttata tatcccatag cttcaagatg tggtgttttt gctaaaacag atattcaacc
     6781 tcttataaat gatggagcca atcagactga tatagcaatg agtattttca atgcagttgt
     6841 cgtacaaact gtgagtgtcc tttcttgtgg aagaaaaata gaaggaaatg ttgcgttttt
     6901 gggtggtcca ttgtactttt tatctgaact tagagaagca tttaaaagag tattagattt
     6961 aaaagatgaa aacataatct tcccagaaaa tgcacaactt tatattgcta ttggagcagg
     7021 attattatca gtagatgaag ataatataca actaaaatct ctaatagaaa aattggatag
     7081 tataaagaat attgaagatg gagaagtaaa tttattagaa cctttattta aagataaaaa
     7141 tgagtatgaa gaattttcaa gaagacatga aaaagaaaaa ataaattatg tggacataaa
     7201 ttcaataaaa agcaattgtt atttaggaat tgatgcaggc tcaactacaa ctaaagcagc
     7261 acttattgat gaggatggaa gacttgtata ttcttattat aatagcaatg aaggaaatcc
     7321 attaaaaaca accattaaag ttataaatga agtatatgat atattaccaa aaaatattaa
     7381 aatattgagt tcaactgtaa ctggatatgg agaaggtctt attaaaaaag ctcttaaaat
     7441 cgataatgga gagatagaga ctatagcaca ttacaaagct gctaaatttt tcaacaaaga
     7501 tgtagatttt atacttgata ttggtggtca agatatgaag tgcctaaaaa taaaagatgg
     7561 agtaattgat aatataatat taaatgaggc ttgttcatca ggttgtggct catttttaga
     7621 gacatttgca agttctttat ctatgacaat agaagaattt gcgcatgaag gtatttattc
     7681 tcaaaatcct gtagatttag gttcaagatg tactgtattt atgaattcta gagtaaaaca
     7741 agctcaaaaa gaaggtgcaa gtgttggaga tatatcagca gggctatctt attcagtaat
     7801 aaaaaatgct ttatttaaag taataaagat tagagattta aatgagattg gtaataatat
     7861 tgttgtacaa ggtggaacct tttataatga tttggtgctt agaagctttg aaaaactaat
     7921 tggaaaaaat gtaattagac ctaatatttc aggaattatg ggagcttttg gttcagcact
     7981 tatagctaaa gaaaaatatg aaagtggata caaaacatcc ttgttatcta aggaagagtt
     8041 gaataatatc aagctagaag caagtgtaac tagatgtaaa ggttgttcaa atcattgtct
     8101 tcttacaata aataaatttt ctgataatga aatttttatt tcaggtaata gatgtgagaa
     8161 gggtgaagct atttatggtg ataaaaaaat aggattagaa aagaaaaaac caataaattt
     8221 atttaagtac aaatacaata gaatttttag atacaaacca ttggaaaaag aagaagcaaa
     8281 aaatggggaa atagggatac caagagtatt aaatatgtat gaagattatc cattttggtt
     8341 tacttttttc aatgctatgg gctttagagt tgtactttca gataggtcat caaaacagtt
     8401 atatgaaact ggtataacta gtattgcatc tgaaacagtt tgttatccag gaaaattagt
     8461 tcatggacat atagaaaatt taattgcaaa aggtataaaa aatatatttt atccaagtgt
     8521 aacaaatgaa aataaagagg attcaaatgc agataattat tataattgtc ctgttgtaat
     8581 atcatattct gaagtaataa aaaacaatgt agagaatata agaaataaga acataaatta
     8641 tataaatcca tttataagtc taaatgataa ggaaaaattg aagaaaagac tctatgatga
     8701 attatcaaga catttttctg atataaaaat aactaaagaa gaagttaatc atgcagttga
     8761 taaagctact gatgaacaga atttatttaa aatggagata caagcagcag gtgaaagagc
     8821 acttaaagaa ataaaagaaa gaaatatgaa ggggattgtg ttatgtggaa gaccatatca
     8881 tatagaccca gaaataaatc atggaatgcc agagcttata aattcattag atatggcagt
     8941 gcttactgaa gactctatat gtcatttagc caatgtacaa agaccactta gagttgtaga
     9001 ccaatgggta tatcattcaa gattgtataa agcagctagt tttgtaagag ataaaaacta
     9061 tttggaactt gttcaactta attcatttgg ctgtggatta gatgcagtaa ctacagacca
     9121 ggttcaagaa atacttaatg aaaaatcgaa gatatatact attataaaga ttgacgaagg
     9181 caacaattta ggagcagcta agataaggat gaggtcctta aaagcagcta tgtttgagag
     9241 agaaagcaaa aatatagatg taaaaaatat aaaggtaaac aggatacagt atgctaaaaa
     9301 taatcagata acgaacaaac atactatatt agcaccacaa atgtctccaa tacatttcca
     9361 attccttgaa aaagctgtta acttaagtgg ttataatata gaaattctaa aagatactga
     9421 tagtagtgta attgaagaag ggttaagata tgtaaataat gatgcatgtt atccagctat
     9481 aatagttgta ggtcagctaa taacagcatt aaaatctggc aaatatgatt taaataacac
     9541 ttctgttact ataactcaga ctggtggagg ttgtagagct acaaattata ttggattctt
     9601 aagaaaggct atatacgatg ctggatttaa agatgttcct gtcattgcac ttagtgtaaa
     9661 tggaatagaa gatagtggta taatggataa tatatcacta aaattaatca atagactctt
     9721 tatgtctgtt gtttatggtg atttgctgat gaaagtacta tatagagtta gaccatatga
     9781 aaaagttact ggaagtgcaa atgctctata tgaaaaatgg gttgatatat gcaaatcttc
     9841 tcttgttaaa gcaaaaattt ctgtatttac tagaaatata aaagatatag taaaagaatt
     9901 tgataattta gagatactag atatcaaaaa accaaaagta ggtttggttg gagaaatatt
     9961 ggtgaaattc catccaattg caaataataa tttagtagat atattagagc gagaaggtgc
    10021 tgaagcagtc gtgccagacc ttacaaattt cttcttaagt tgtgccttta atactatata
    10081 caaacacact catttagaag gaagtagaaa gagtagaatg ataggagagg catttatata
    10141 cataactgga atatatcaaa gagtatataa aaaagcactt gataagagtg agagatttta
    10201 tgctccagcc aatataaaaa atgttgctaa aagtacagaa ccagtagtgt cattaggtaa
    10261 tcaaactgga gaaggttggc ttttaacagg tgaaatggtt gaattattaa atgaaggtgt
    10321 agaaaacatt atttgtatgc aaccttttgg atgtttacct aatcacataa ttggtaaggg
    10381 ttctataaaa gaattaaaga ggttgtataa aaatgcaaat ataataccta ttgattatga
    10441 cccttctgca agtgaggtaa atcaacttaa caggataaaa cttatgcttt ctaaggcttt
    10501 taaaaatata taagatttat attttgatat gttaaaagtt aatgaattta aaaatatact
    10561 ctatattaag tatctataaa aaagagttat cctatttttt agggtaactc tttattagtt
    10621 aaaaaattat atgtatactg atattaaagg ggggaagtaa tatgagttta gtatatgatg
    10681 tttataaatc aaaattagga aatttgtaca tattaagtga agatggtttt atagttagta
    10741 tatacatagg agatgaaaaa tttaataatt taaaaaatga atgtattaaa aaaggaattg
    10801 atataaagag aagtaaggaa gatttaaagg aagcaataag ccagatagat gattacttta
    10861 atggaaaaag aaaagtcttt aatttaaata ttcgtattga agggactgaa tttcaaaaat
    10921 cagtttatag ggaaatgtta aaaatacctt atggagaaac attgtgttat tcagatatag
    10981 caagaaatat aaacaatcca aaatcagtaa gagcaatagg acaagcaagt aaaagtaata
    11041 aaattccaat aataatacct tgtcatagag tagttggcaa aaaagatatt ggtggatata
    11101 tggggaatca ttctgatttg aaagagatac tattaaattt agaaagacag agttaactta
    11161 gaaagaaatg tttttattgt taatatttga tataataata gtaaaaagaa gtatttttta
    11221 caaaaacaat gaagaattta ttttaattta caaaaatgag ggggaatttt aatgtgtgat
    11281 aaagttatag catcaaaata tcatgagaag ggatttaatt gtgcagaatc agttataaaa
    11341 gcatataatg aggaatttaa tacagatata ccagtttgtt taggaagtgg actgggttca
    11401 gggtgtgggg ttgctagttt atgtggtgca gtaaatgcat caaatataat aattggatat
    11461 gtaaaaggca gaaaccacga agatgaaagt actaaagcta aagtatatgc taaagattta
    11521 actacaacag taagaaaaga gtatggttca gagttatgta tagacctaaa aaaagattta
    11581 gttgcttgta gagaaataat ggatttcgca tatgatagtc taaaggaaac tttaaaaaaa
    11641 gaattataag aaaataagct atcaaatata aattatttga tagcttattt ttatagccga
    11701 tactaaataa atcaaatata ttttagaatt attatttgta aataaacagc aaataatttt
    11761 aggcttttta tttaatatag tttttattta tatttatttt tgaatatgtt atactttgtt
    11821 taaataaata tttgtaatga gggagatgag aagattgaag aatctcaatt tacttattaa
    11881 taaattatat tcaaaaaatc ataatgaagc atataaaact tttttattct tagaaaatga
    11941 aagtttaaaa tcaaatatta catattgttt ttttgactca ttcctagaaa tgattgataa
    12001 cgaaaactca tatattcgaa caaggggatt gttattaatc tctgcaaatg ctcagtggga
    12061 tattgataat aaaattgaaa taaatataga tagtatacta agtcatatcg tagataaaaa
    12121 accttctgta tcaagaatgt ttattaaatc catacctaat attacaaagt ataaagaaaa
    12181 tcttattaat cgcataaaaa tggaattaag taatgcagat ataagtattt a
//
LOCUS       DAEMHZ010000123        11974 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000123 DAEMHZ010000000
VERSION     DAEMHZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.123"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..276)
                     /locus_tag="KPK72_003009"
     CDS             complement(<1..276)
                     /locus_tag="KPK72_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HBG2490203.1"
                     /translation="MRKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNI"
     gene            complement(305..1855)
                     /locus_tag="KPK72_003010"
     CDS             complement(305..1855)
                     /locus_tag="KPK72_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2490204.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            complement(2057..2941)
                     /locus_tag="KPK72_003011"
     CDS             complement(2057..2941)
                     /locus_tag="KPK72_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2490205.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3169..4176
                     /locus_tag="KPK72_003012"
     CDS             3169..4176
                     /locus_tag="KPK72_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2490206.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            complement(4388..5086)
                     /gene="yunB"
                     /locus_tag="KPK72_003013"
     CDS             complement(4388..5086)
                     /gene="yunB"
                     /locus_tag="KPK72_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG2490207.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            5300..7993
                     /locus_tag="KPK72_003014"
     CDS             5300..7993
                     /locus_tag="KPK72_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2490208.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKDDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            complement(8268..9203)
                     /locus_tag="KPK72_003015"
     CDS             complement(8268..9203)
                     /locus_tag="KPK72_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG2490209.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(9247..10419)
                     /locus_tag="KPK72_003016"
     CDS             complement(9247..10419)
                     /locus_tag="KPK72_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2490210.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(10406..10870)
                     /locus_tag="KPK72_003017"
     CDS             complement(10406..10870)
                     /locus_tag="KPK72_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490211.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(10885..11727)
                     /locus_tag="KPK72_003018"
     CDS             complement(10885..11727)
                     /locus_tag="KPK72_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2490212.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         4054 a         2023 c         1563 g         4334 t
ORIGIN      
        1 tatattcctg acttcttcaa aatgtatctt cccttgtgga gaagatttta atgttggtaa
       61 tttgaactca gacacattct tatttaagtt ataatcaatt agataatcgt agttcaattt
      121 tatatcatct ttagataaac cagataactc tgatatattt aagtaatcaa tgtcaaaata
      181 ataaagttgt ttaaatccaa cagtaaaatt aataacacct actattataa caatactaat
      241 acaaattgaa aaaagtacat ttagaaactt tctcatgaaa tccccccagc aataagttaa
      301 atcactacac gattttagtt gtaaaatctt ctatattcca tacatcatca acaatttctg
      361 aataaaattc tggctcatga catactaaaa gaactgttcc cttaaactct ttgatagcct
      421 tcttcaattc atcttttgcc tcaacatcta aatgatttgt aggctcatct aatactaaga
      481 aatttatatt ttttaacatt actttgcaaa gtctaacctt agcagcttct ccaccactta
      541 atactctcat ttgactcgtt atatgttcat ttgtaagtcc acattttgca agagcctgtc
      601 taacttcaaa gtttgtaagc cctggaaatt cactccaaac ttcatccata ggtgtattac
      661 tattttctct tgaactctct tgttcaaaat atccaacttc caaatagtca cctaatttaa
      721 catttccttc aaatggtttt attattccaa gtaaagtttt taatagagta gattttccaa
      781 taccattcat tccttttaag gcaatctttt ttcctctttc taaatggaaa ttaagtggtt
      841 ttgtcaatgc ttcatcatac cctaaaacta aattttctgt ttcaaaaatt attttactag
      901 ctgctctagc atctttaaaa gcaaaagtag gttttatttt ttcttttggt ctttctaata
      961 tttccatctt atcaagttgc ttttgtctac tgttagccat tcctctagtt gctactctag
     1021 ctttatttct agctacaaaa tcctctagtc tttttctttc ttcaacttgt ttatcataag
     1081 cttgttcttc ttgacgtttc tttatatcat taagtcttac aaattcatca taatttcctt
     1141 tatatctatt taactctcca ttttccatat gatatattac attacatgta ttatttataa
     1201 attcaatatc atgagaaact aagacaaaac tattctcata ctcttgaagg tacttagtaa
     1261 gccatgttat atgctcttcg tctaaatagt ttgtaggctc atccaatata agaatagttg
     1321 gattttcaag tagaagttta gtaagaagta cttttgttct ttgccctcca cttaagtcag
     1381 ttacatcttt atcaagacca atttctccaa gaccaagacc atttgcaact tcttgtatct
     1441 ttgcatctat catataaaaa ccactatttt caagtatagt ttgtatctct gctgtttctt
     1501 caagaagctt actcatttca tcatcactag cttctcccat tttgtcatac atagcaatca
     1561 tttcttgctc taagtcaaac atatgcttaa aagcctctct aagcacttct cttatagttt
     1621 ttcccttgct taataccgta tgttggtcta agtaacctac tgtagctctt gaagaccatt
     1681 ttatatttcc agcatctggc ataagcttct ttgttattat atttaaaaaa ctcgattttc
     1741 cttctccatt agcaccaact agtgcaatat gttcaccttt tctaagtcta aaggatacat
     1801 tttctaatat tgttctcgca ccaaatccat gactcacatt ttctactact agcatttcaa
     1861 cacctctcaa aaaaatttta cacattctat actataatat attcttttta aacttaaatc
     1921 aagtattccc aatattttta gttatattta cgacaattta acctaaaata aacttcttct
     1981 ttgtgttatg tacaataaaa aatagagata aaaattaggc aaataatacc aacctaactt
     2041 tttctctatt catatcttat ttaaaatacg catccaatcc atcagctata cactgcatca
     2101 tttttctttg gtagtctgga tttgacatca tttggtcttc attatagtta ctcataaatc
     2161 ccatttctac taatacagct ggtacttttg accagttgaa tccagtcaaa tcacctcttt
     2221 gaacgattcc attgatttgt atacctgatt gttccatatt ctgtttgata aattcagcac
     2281 atttattact ttcttcatat ataggagctg tatattttcc atctttttca ggaatcaata
     2341 tagatgctcc agtttttgaa gaattattta aactatctgc atgtattctt acaaccatat
     2401 ctgctttttt gtcattagca agtattgctc tttctgaatt acttatattt acatcatgtg
     2461 tctctctagt cattattaca ttataacctt tagattctaa tatactcttt aaaacaagag
     2521 atgcttctaa gtttaaaaca tattctggct tttttgtagc aataccctct gttcctgaag
     2581 atactctcgc ttttttagag gaagaacctg gagctactgg ttctaaattg ctatctcctt
     2641 ttccttgatg ccctgggtct atacaaatta aaagtttttt atttttttga atttcatttt
     2701 tattatctga cgatttgtcc tctgatgact tatctttatt acttttagaa ttagatttgt
     2761 cttcattttt atctttatta tttttgtttg aattttcatc tttatctgct ttttcatttg
     2821 tgatatttcc tgaactaatc aaatttgtag tcttgtaaaa attgctaact ggatttgatg
     2881 ttcctataat tatgattccc atggcaatta tacaagtaac taacaatcta tattttttca
     2941 ttttttgctc ctttcttatg ctagacagtt attgtttatt tttatttata tgaatatgta
     3001 tttattttat catatacact atttatttta tttattttta tgaaaactaa ggtgttattg
     3061 agctagtttt ccaagcatca aaacatgatt cttgcacaac ataatataga agcagcaaga
     3121 aaacttcaat tctattatta aaataattta ataagaaaga ggtgttttat gcaacttaaa
     3181 aattttcatt ttaaaaataa tactttaagt aaaaaaacca acttagctaa gtcaaaattt
     3241 acaattgttt taacattttt atttttatta tattttttct caaataattt aatttatgca
     3301 caaaatttac aaaaaaatga acctgaatat ttaataccaa ttggaaacgt acttcaaatt
     3361 gacgcagaat taaaaaatat catagttaga aatcctggag aagcatctcc atttagattg
     3421 ggtgatgcta tagtaaaggt aaataatgct gaagttgatg gatatagtga ctttgccaat
     3481 accttaaatg ctttaccaga agaaaaacaa gtatctattt tattaaatag aggtggacaa
     3541 cttataacaa tgaaaactac caaacacata ttagaaaaaa ttagttttaa caatttactt
     3601 tctggatttg ctactttaac gtatatcaat ccagatacat cagagtttgg agctgtaggt
     3661 catccaataa gtgttggtaa tgcaagaaaa atacctatta aaacaggttc aatttctaca
     3721 actaagcacc ttaatataga aaaatcttat aaaggtagtg ttggatgtat aaatgcaaaa
     3781 cgagaaaata ttattggtag ctttacagaa aatactgatt ttggaataag aggtcaaatt
     3841 aataactttg atatttccaa gttaaaaaaa tataaagtag catctttaga tgaagttaaa
     3901 ttaggaaaag ctcaaataat tttacaagat aactttaata agtgtaaaaa atataatatt
     3961 gaaattatag atatagaaaa ccaaaaacat ccagaaagta aaagttttaa aattaaaatt
     4021 acagataaaa agcttttagc tcaaactggt ggtattgtac aaggtatgag tggtactcca
     4081 atagttcaag ataataaaat aatcggagca gtgtcacatg caattgaaaa cgacccagct
     4141 gtaggttatg gtgtatttat taaatggatg ttgtaaataa tataaataaa taagttttaa
     4201 gatgttatta agttattaat aatatatttg gtctaaaggt acttttattt aacctacaga
     4261 ccaaatatat tagatatttt acatagtata aaatataaat ttttattaaa attatattaa
     4321 tattatttcc aaaaatttta tttataaact attattttga atttatttaa aaatatacaa
     4381 ctttacatta cttgttggta ttctttccag tgttaggtaa aacatttcct agattttttt
     4441 ctggaatatt tacataactt tctggaacat cacctactat tatagtttca caaataggta
     4501 tttgagcttt aatttgtttt gttgaagtta taagtggtat tactacttta acttgtgttt
     4561 gagcttctaa atatattcta tgtctagttt gatttatacc tgaagactca aattccgttt
     4621 taaagtttac tgatactgtt cctataggtt caatagatac ttcaagttta ggaccgtatt
     4681 tagctaatat aggacttcct aatgctgttc ctataggtat gtaagaactt gctgtcttat
     4741 ccttttttaa ttcatcttgt atttctaaag ctattgcaga tgcaatttca ttcatcatta
     4801 tagtgtttgc ttgtatcata gttattttac cattattatc aagttgtata tccattaaat
     4861 cttcataatt tatttttcct tctaccatct cagcaacagc tttatttatc gacctatttg
     4921 ctagttcaag agctttagtc tctgctaaaa ctgttattgt cggtcttaaa gttttatcta
     4981 tatatataaa actgcctata aatacagaca agaaaagaat tactaaaaaa atagctatga
     5041 tttttctaaa ttcatttttt gtttttctat ctactattct attcaaaatt atcccctcct
     5101 atttatttat atgtagcttt ggcataattt ttataatatt tgtcaaaaat aaataaaata
     5161 aaaatataga ctttttaata tattacgttt atacatatac aatgtacaga atattatagc
     5221 aaattcattt tttctattaa ttacaaaact actcctattt gtgttataat attgcattaa
     5281 gtaaggaggt taaaatctta tgaataacga taatgataaa aatggaaata aaatcaggag
     5341 aaaaaaagtt agctcatctg gtaatacaaa taaaccaatt aatagaactt cagctaacaa
     5401 gactcgttca acaaaaaaca agaaaaaatc taaaaaaagc gataaattta aaaaactaag
     5461 agtatttggg atagtatttt tagttttact agttgttggt acagcaggta ctgctggtct
     5521 agtatttgcg tcactaagag atgtaactcc agttacagaa gctgttttag ataaacagac
     5581 gaaccaaaca actactatta aatacgctaa tggaaaaaca ttatctacag ctccaagtgt
     5641 aaataaaaag acacccgttc cattagataa gatttcccct tatcttcaac atgctgtaat
     5701 tgcaatagaa gatgagcgtt tttatgagca taagggtgtc gatattaaag gtttatttag
     5761 gtcagtatta aagaccctta caggtactaa gcaaggtggt agtactattc ctatgcaggt
     5821 atccaaaatg ctgttaacaa ctgaacagca aactatacct cgtaaaataa aggatattta
     5881 ctatgcacat gagatgagta aaacagtaag taaagataaa atactagaaa cttatttaaa
     5941 taacttcttc gttggtagag gtctagcagg tgctgaggct ggtgcacgtg gttattttga
     6001 taagtctgca gctgacctta ctctagcaga gtcagcactt cttgctggtt caactaaaaa
     6061 tccatcaaga ttttcagcct ataaaacttc taagttagag ggaaatgaaa ctaaggatga
     6121 tttagagaat aaattattat tttttgttaa cacaactgat gatgatttag atgaccctac
     6181 tcaagtggac tttgatatga ttgaaaaaat aaaatcttgg gagcttatat ctaatgatac
     6241 ttatagacaa ttaaaagcag gtacattagt tgttagaaag gctgtaagta atcctgaagc
     6301 taaaaaaaga caagaaattg ttttaaagaa aatgttagaa ttgaaatata taaaacaaag
     6361 tgaatatgat gaagcaatca aagctaaaat agaaataaaa ttgcctcaat catcagataa
     6421 agtttcttca tctgtagaag accttatcga aagtgaagtt ataaatgctt taatggaaca
     6481 aggacataca aatgatgaag ctcaaaactt attctacaat ggtggtctaa ttgtaaacac
     6541 aactatagac cctaaaatgc aagatgcttt agaagaagaa tttgatagaa atagcaattt
     6601 cccaggacat atggtcggtc ctgatggagt tagccaaccg caagctgcaa tggtaatctt
     6661 agattataaa aatggtgaaa taagagcact agctggagga agaaatataa gtggtagaaa
     6721 aactttaaat cgtgctacta accctcatca gccaggttct tctataaaac ctttagcaat
     6781 atatacgcca gctattgata ctctaaaaat aacacaagca actgctctaa gtgattctcg
     6841 aggaggatat aagtttaaag aaaataatga atggaatcca agaactacta ctgctggtca
     6901 tggttctatg agtttacgta aagcacttgc taaatcttca aacacgatag ctgtcaaaac
     6961 tgctgaaatg cttggagact cttatgatga atgtgtagat ataatgatgg attacttaaa
     7021 gaactttggt ataacaactt taaagaataa tcactcaggt tcatctgaag caagtgatag
     7081 aaaattccca tcattgacac taggtggtat ggcgaatggt attactccac ttcaaatggc
     7141 tgctgcttat ggtacattgg caaatcacgg tatttatgta gaaccttcta tttttactac
     7201 tataactacc tttgatggtc aattattagt taaaaatgct cctgaagaac ataaagttgt
     7261 agaccctgaa gtagcttatg ttgttacaga tatgttagaa agcgtaatta ctgaaggtac
     7321 tggtggtgtt gctacacttc caaagggaat gcctgtagca ggtaaaacag gtactacaaa
     7381 tagtgcatat gatgcttggt ttgtcggtta cactccttat tatgttggtg caacttatat
     7441 tggagatgat gctggtagaa aagatgattc tggtaacact ataaaacgta gagaagtacc
     7501 acatggaagt actagtactg caaaactatg ggaaaaaatt atggaaaaaa tacatgctaa
     7561 tttaacggtt actgaatttg aagttcctaa aaatgtatat tttacaaaaa ttaatttaga
     7621 ggatggagga aagcaatctt ctggctctaa agcagcattt attgaaggta ctgctcctac
     7681 taaagtaagt tctcaaccat catctgaaga tacaaaaaaa ccagacaata accaaccaga
     7741 agaaaataat aataacaata ataacacagg tgataatggt ggcggttcta cacctcctga
     7801 taatggcgga aacaatggtg gtggctctac tactcctcct gataatggcg gaaataatgg
     7861 tggtggctct actactcctc ctgataatgg cggaaacaat ggtggtggct ctactactcc
     7921 tcctgatggt ggtggaaata atggtggtgg ctctactact cctcctgatg gtggtggaac
     7981 tccagcaact taataaaaaa tagaatttaa ataaaaatat aaggagttgt ctcaaggtga
     8041 ttaaaaaaat cactttgaaa cgctcctttt tttataatta caatatatta tttgtattca
     8101 taaaaaagag aatcccaata accagaatcc tcctttttta tataaacaaa taaatatttt
     8161 atttttaaag tttttaacta agtgctttat tcttactttt attcctaatt gtttatatat
     8221 tcttaaatag ttatatcaca aattatataa acttaatatt tttctaatta ctgtatacca
     8281 aatgtttgaa atctatttaa tttgccatat ctcttaataa gcatttccca ctcttcttta
     8341 tcatagaaat cacacttatt tctaccaaat agcttggcaa cttcaattgc aaatctccca
     8401 gctgaatcta aatcactaaa attagttgca cctgtagcac atccagcaac aggcatctct
     8461 gttgtaattg ctactcctac tattggtgca tctgtagcta ctgctggttg taaaatacta
     8521 tttaagtgat acagattatt tccatatggt gttatatctt gaatagacaa agggaataca
     8581 aatggagatt tccctgttac agtctgcata atatctaaaa ggttatcact agtttttaat
     8641 atgtagcctt ctttaacagt tggcgatatt gcaaatcctc tagtattgat tactctattt
     8701 cctttagtag tatctataga aagtattgca tcacaactac tatcaacttc attttcattc
     8761 atagtcatca tatcaattgg agaatccata aaaggagtag gatagtgttc tttagttggt
     8821 gcatctgggc aaatatgggt tgaaattaca acatcaccat ctaatatatc cccttttcgt
     8881 tgcatatcca aaagtttagc agccactgca attgctacca atgctccatc accatctgaa
     8941 acaaaaccta taacttctgg tcttgctcca atccctccta gtctacctaa aatgccaaga
     9001 gtaggagcat ctccaccttt taattttcca tttttcccag gaattgtaag ctttactaaa
     9061 tctgttgagc ctttacttga agacaattct tttacagtta catctacttc accataacct
     9121 tgtaagtaag tttttacttc ttccccatta gcatttgctt tatctaaaat gtcataaacc
     9181 tctattactt gttttaatat catgtctaat ctgatttcct ttcttaatat attttaaaat
     9241 tatatattat ttactataca aaatatctac tccacacatt gctagcacct taattgcttt
     9301 tataagtgct actctgcctt cttcaccacc agctgcaaca gcaaattcat ctgtatgagt
     9361 tgcagtattt tcttttaatt ttatatatgc ttgtattgca ggtatctcat gagttaaatt
     9421 tcctgtatca gttgaaccta taccttgact taaatctctt tcaacatagt cttcacccaa
     9481 aacttcaaaa ttttgtctaa ctatattcac taattcagaa tcattcttaa tctctttgta
     9541 tatttcatct aattgaacta tttctacatt agttccagta gaaattgcag ctcctttagc
     9601 acaattttta agcatttcac aaatctcatt taaatattct atacttaaag ctcttacatt
     9661 aaatatagct gtagattttt caggaattat attatgtatg ctacctccat cagttataat
     9721 tccatgtact cttgcataat ctttaagatg taatctcatg ctatttacac tgttaaatag
     9781 tgctattact ccacttaagg cacttttccc ttcccaagga aaagcagcag catgtgatgc
     9841 cttaccagta aaatcaaatt ttaaattaac ttgagcaaat gatatatcat caggcattga
     9901 agcatcactt ggatgtaaaa ttaatgcagc atctacatta ttaaatacac cttctttaat
     9961 catagttatt tttccaccta ctctttcttc tgcaggtgtt ccaaaaacct ttacagtccc
    10021 ttctatatta tgttttttca tgatttcttt taatatgata cctgcaccaa cagaacttgt
    10081 ccctatgata ttgtgcccac atgcatgtcc cattcctgga agtgcatcat attcagctaa
    10141 aaaagcaact gttttcccat tagttccatt tgaatacaca gcttcaaaag atgtttctaa
    10201 tcctcctaca cctttagtta cattaaattc attttcttcc aacaaatcac ataatgcttt
    10261 ttgtgactta tactcctcaa atgctaattc tggatttgaa aataaatagt cagaaacttt
    10321 ttcaaatgat gtctttttct cttcagataa taactttatt tcctctttaa tttttgcttt
    10381 taattcattc atatttacta cctccttaaa gcattccatt taatcttaaa actgtttgag
    10441 atataaaagc agaaccaaag taagtagatg tcataactat aaatgatata actactaact
    10501 tccaactcaa agtctttaat atatctaatt ggttacctac agagattcct gcaaaagcta
    10561 ataatggtgt tactgtagac ataaaattga ttttccctac atttgttgct atgatatccc
    10621 ccactggaga tattggaagc gataaaacga atgcaactaa tgtagcccat gcaaaacctg
    10681 gtaaattagg ttttttaaca aaattcttta ttataatagc aattatcacc aacaaaaatc
    10741 cagatacact tccaatcaac aaatcttgag cctgagtctt aagtgctatc atttgtccaa
    10801 agcaaataat aatatatata aacaatacta tttttatctg ctccataata gtcttaaaag
    10861 ttttttccat atatatatcc tcctttacaa tttattactt gttcttcttg tttttcttcc
    10921 tctaaatctt tcaataactg gagctacttt gctataatag tagttggcta ctggtaaaga
    10981 taagaataat tctatatata ttccagtaac agctgtaatc aatccactag tagctgaata
    11041 tgctaaaatc tgctctgaca tttgtggaac cgtattcatt aatgatgctg ttgcagctcc
    11101 catcattgaa ccactaccca ttcctgatgc cattgctaaa gcatatggat gaaatcctaa
    11161 aagtacaccc actggtgcta aaaatgaaaa taataatgtt ccaaatatac taccacttat
    11221 gtaagttcct agtgttccta taccttctgg tgagtttaat ccatacttat ctccaataat
    11281 tcctagtgct gtgtcacgac ataatgaaaa agtaccacct aatgcacttc ttccaagacc
    11341 caatgctact ccaacaggta atgctatcaa cattgtaccc atatgaccaa attcttgtaa
    11401 taaaagtgct ggtcctgctt gaagtaatat accaacttta ggtcctgcat taattccaaa
    11461 cattatccct aatggatata gtgtcaaaga taataaatat ggagatacat ttatttcatt
    11521 atcccctata aactttttta aggctggtat aactctccct aataaatgag gagaaacagc
    11581 cattcctatg aatatagccc atagtatact aagtaaagtt acattaattc ccataacttt
    11641 aaatgaaata ggtccaatta attctgccaa tattgtgatt ataaaggctg ctattatggc
    11701 atttctaatc cctgcttttt ctttcatttc tatacctcct tgtaattttt ataattttct
    11761 tataatataa ttgttagtga ttttattata taacctatca ttgataatgt ataatatata
    11821 tttattcatc atttttatat acttttagtt atgaatcaaa aaaatattat agattcatga
    11881 tataaaatac aaataattta ttaatttata ttgttaaaat aacggatttt caaataattg
    11941 aataaaatta ttcattcata ttataaaaac tatc
//
LOCUS       DAEMHZ010000124        11828 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000124 DAEMHZ010000000
VERSION     DAEMHZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.124"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..1227
                     /locus_tag="KPK72_003019"
     CDS             187..1227
                     /locus_tag="KPK72_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490213.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1582..2904
                     /locus_tag="KPK72_003020"
     CDS             1582..2904
                     /locus_tag="KPK72_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2490214.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3201..4562
                     /locus_tag="KPK72_003021"
     CDS             3201..4562
                     /locus_tag="KPK72_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2490215.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4635..6029
                     /locus_tag="KPK72_003022"
     CDS             4635..6029
                     /locus_tag="KPK72_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2490216.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6091..6561
                     /locus_tag="KPK72_003023"
     CDS             6091..6561
                     /locus_tag="KPK72_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG2490217.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6564..8780
                     /locus_tag="KPK72_003024"
     CDS             6564..8780
                     /locus_tag="KPK72_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2490218.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8863..9534
                     /locus_tag="KPK72_003025"
     CDS             8863..9534
                     /locus_tag="KPK72_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2490219.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9897..11111
                     /gene="dpaL"
                     /locus_tag="KPK72_003026"
     CDS             9897..11111
                     /gene="dpaL"
                     /locus_tag="KPK72_003026"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2490220.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11238..>11828
                     /locus_tag="KPK72_003027"
     CDS             11238..>11828
                     /locus_tag="KPK72_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2490221.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4470 a         1355 c         2402 g         3601 t
ORIGIN      
        1 aatagaggat ttttgtaaaa tgaagaaagt atatctatca aattaaaact aagtagatat
       61 actttttatt aagttttgtt ttaaaataat attattaaat tataactaag cttttctgtt
      121 atgatataat taaatagata atgtgaatga ttattatata gaatacttat ttggagttga
      181 tattttatgg acaaacacag tgattcagta atgtatggtg gaatggaagt atataatatt
      241 tggggaacaa caacagtaga ctccataatt ggagagtctg atgtaatgcg tgctcttaag
      301 aatagaattt gtaaaattgg aaatagtgat tctactgtag ctataactgg agaaagtggc
      361 actggaaaag aattaatagc aagagccatt catctcgcag gaaacagagg gaataaaaca
      421 ttcgttgcta taaactgtgc tgctatacca gaacctttac tagaaagtga actttttgga
      481 tatacaaaag gagcttttag tggagcagat tcaaaaggta aaattggtaa gtttgaattg
      541 gccaatggag gggttgtatt tttagatgag ataggagata tgccaattca actacaatca
      601 aagttattga gggtattaca ggaaaagaag tttacgagaa taggttcaaa tgaactaatt
      661 gatatagatg ttagaatcat atcagctact aatagagatt tatttgagtt ggttaaggaa
      721 aataaattta gagaagactt atactataga ttgaatgtaa tacctttaga agtgcctcca
      781 cttagagaaa gaggagacga tatacaaata ttaataaaca attttataga caaatattcg
      841 agaaagatga agaaaaacgt gtaccatata gagccagatg tggaacaaat gttgcttaag
      901 tatccttggc caggaaatat tagagagttg gaaaatacta tagaactggc attaaacctt
      961 cttgaggaag atggaataat acataaagga ataataaata gaaagatact tgaatatttt
     1021 aagttaaata atataaatat aaagttactt gaggaaagtg actatgagat aagtatggaa
     1081 caagacatac ttacacttga agagtttgaa agagcctata taaagaaagt gttaaagttt
     1141 tacggaaatg atactaagtc taaaaaactg gctgccaaaa aacttggaat atcactagct
     1201 acattgtaca gaaaaataga tgtataataa ttaggctttt tattctcaat tctaaaattt
     1261 gaaattataa aattgataaa aaacacttgg aaaactattt tctaagtgtt tttttgtgta
     1321 atagcatttt attatccaaa aataaaaaaa atttaaaaga atttaaaaaa ataaatgctt
     1381 taataaatta tcaaatacta acaaatataa taaaaattat atatgtaaaa caattatgtt
     1441 aaaaaattgg cattataatt gcttcatatt aagttgagat gacaaaataa aaaaagaaag
     1501 gatgttgtgt gagataataa taactgtcat taaaaatagt tgggagtgat aaccttgagt
     1561 aaatatcaaa agaatgtttc tatgtttgat attgatggaa ggccaccaat tttaaagtgt
     1621 ttgccactat caatgcaaca tatactagca atgatagttg gaactgttac agtacctata
     1681 gtcgtaggag gagctgtggg tgttagccct acacaaataa cacttttagt gcaatatgca
     1741 ttaataatag ctggtatatc aacacttata caactatatc caataggaaa tattggttca
     1801 agattgccta taatttttgg agttggtttt acttatgtac caacacttgt agcagtcgga
     1861 ggaaattatg gattagcaag cattttgggt gcacaattaa tagggggagt ggctacagta
     1921 ttaataggga tattcataaa aaagataaga aaattttttc cgaatatagt tgcaggaaca
     1981 gtggttttta caattggatt atctttgtat cctatagcaa taaattatat ggcaggtggt
     2041 gtaaatagtc ctacttatgc ctctatgcaa aactgggcag tagcggctat aacattagtg
     2101 gtagttatag gtcttagtca atttgcaaaa ggatatgcaa aattggcagc aatatttttg
     2161 ggaattatag ttggatattg tgtatcttta gttctgggta taataaattt tgaccctatt
     2221 agagaggcaa cttggtttgc gatacctaaa ccgcttgaat ttgggatgga atttgatatg
     2281 cctgtaataa tctcagtagt aattgtaagt ataatcaatg ctgttcaagc aattggagat
     2341 ttatcagcta ctacaatggg aggcatggat aggaatatca cagataagga attatcaagt
     2401 ggagtaatag gaagtggaat ttgtactatg ataggagctt tgtttggagg tttaccacca
     2461 tcatcatata gccaaaacgt ggggatggta gctatgaaca aagtaataag tagatttgtg
     2521 cttggaatag ctggtatatt tatgttattg tcggggtttg tgcctaaatt tggtgcatta
     2581 atgactacta taccatattc tgttttagga ggagctacaa tatctatatt tagtatgata
     2641 acaatgacag gtataaagtt aattattaca gaagagttgt catcaagaaa tattaccatt
     2701 gttggtttgg caattgcctt aggcatggga ataacatcag tcccagcagc ccaagaggca
     2761 tttccttcat ttgtaaagtt agtatttgga gaatctccaa tagtaattgc aacattggtt
     2821 gcattcgttt taaatctaat aataccaaat aaaagtctag aggatgaggc taaagaaaga
     2881 gctgttatgg aagagactaa ttaagatata ataatttaaa agaattgttt aaattattta
     2941 gtatgagatt gttagattat ttttgatagc tattcatttt aataaaaaat ttaacaaata
     3001 aatatatacc aggcaattaa tgttcattta agttgaaaat cggggaggca atatttagaa
     3061 atttgttaat tttttataaa aatagctcag tttagctgtg tttatcggtt gagcaataaa
     3121 ttcatggtca taaaaatatt tatagaaaat aattataaaa atatttagtc aagatattta
     3181 tgtatgggag gatttatttt atgaaaacac gtaatacttc aaaatatgat gtcgatggga
     3241 taccaccact aaaggaatca atacctttgg ccttacaaca tctgttagca atgatagtag
     3301 gaaatatggt acctgcgata ttaatagcaa acgtggtagg acttaaccag gggcaagcaa
     3361 caatgttgat tcagggttct atgttagcag caggtttagc aacgttttta cagttatatc
     3421 caataccttt atttaaagga tttaagttag gttcaagatt acctgtaatg atgggaatga
     3481 gttatgtatt tttaggagct tgtctttcag tagcagctaa agatggttta gcagctctat
     3541 ttggagcaca gattgctgct ggggtaatcg tattctttgt aggatttgga gttaagaaaa
     3601 ttagacatat attcactcca atagtttctg gaactataat agcatgtatg ggacttggac
     3661 tattcgcaac agctataaag aatttagctg gaggagaagg cacacaaaca tttggaagcc
     3721 caataaactt cacagtagga gttatagttg cttttgtaat aataatgata aataaatacg
     3781 gtaaaggttt agtaaaaaat tcttcaatat taataggaat attagtaggt tatgctatat
     3841 ctttagtttt aggattggtt gatttctcag ccgttcaagg agcagctata gtatctttac
     3901 caacaccagc agcttttgga ttagaattta gaccagagtt aatagtaatg tttactatta
     3961 tttacataat aggtatagct gatatgatgg gtgcctgtac aatagctaca gtaggagcaa
     4021 tggatagaga ggtaactgac gaagaattag catcagttgt attaggaaat tctataactt
     4081 ctataatttc atcactattt gcagctcttc caactggagt atttagccaa aatacagtaa
     4141 ttgtatctat gaacaaagtt acaagtagat ttgtaatagc actaggggca cttgtattat
     4201 tactagcagg gatatctcca gcattgggtg caataatgac aactatacca tcttgtgttg
     4261 ttggtggagc aactttagta gttttctcat caatagcaat gtctggtttc tctattatga
     4321 gtatggatgg attcactgaa gaaaacaatc ttatagcagg tgtatctata gcaacaagta
     4381 tgggtcttac aactgctcca caagttttag accaattccc agaaacaatt ggaactgtcc
     4441 ttggagggtc aagtatagtt tctggagcaa taatagctat attacttcaa acattattta
     4501 aattaaagtc acgtaaaagt gctgaaaacg ttacttcaaa tcttgaggaa aatattggat
     4561 aaatgtatta gatataatat gagaaaacca tatggtaaaa tgttgtaatg aaagtattca
     4621 aagggggatt taagatgatt gatatactaa taaaaaatgc aataattgtt acagttaata
     4681 aagacagaga tgttatattt gatggagcaa tagctattca aggtgacaga atcttggaca
     4741 taggaaatac agttgatatt gagcctaagt atcttgatgc taaaaaagtt atagatgcta
     4801 atggaaaagt aatattccct ggctttataa atactcataa tcacctgttc caagtgttat
     4861 taaaaggttt aggtgatgat atggcacttc atgagtggtt aaatacaatg atgtttccat
     4921 cagcaaaatt cttaacagaa caagatactt atgatgctgc aatgcttgga tgtatggaag
     4981 gtttaaaaag tggtataact acaatggttg attatatgca tactcacaat agaccaggac
     5041 ttacagatgg aatagtaaag gcatataaag atttaggtat aagaggggtt gtaggtagag
     5101 gttgtataga tttaggtatt cacaaagagc ttatagaaga tgtggaaacg gttgaaaaag
     5161 atttgagaag ggtattcgaa aaatatcata attcagaaaa tggaagaata aaaatctgtg
     5221 tagctccttc ttcaatgtgg gctatatctg aagaaatggg taagatgctt tggaatatag
     5281 ttaaagaata tgattcatat tttacagttc atatatctga aacagaattt gcaagaacag
     5341 caacaaagga tatacatgga gaaatagata taaaattact tgaaaaatgg ggtatagtag
     5401 gtccagaagt tgtagcagta cattgtgtat gtataactga tgaagacatg gaaatgctta
     5461 aaaagtatga cataaaagtt tctcataatg tagcaagtaa tatgtatctt gcttctggtg
     5521 ttgcacctgt gccagaaatg ttaaaaaaag gtataaatgt gagtttaggt cttgacggtg
     5581 cagcaagtaa taacgcacaa gatatggtag agcttatgaa attaacagct ttacaacaca
     5641 aagtaaataa ttgtgaccct ctggcaattt ctgcagagaa agttcttgaa atggctacaa
     5701 ttgagggagc aagaactctt agaatggaag atgaaatagg ttctttggag attgggaaaa
     5761 aagctgacct agtaatattt aactcaatgc tttctccaaa atcaatacct cttcacaatc
     5821 ctgtatcaac tttggtatac tcagctagta tgcacaatgt agagagtgtt ttagttgatg
     5881 gaaatgtaat acttgaaaat ggaaaagtaa ctacaattga aagtgaagaa caagtgttgc
     5941 ttaatgcaca acatgcagca gaagaattat gtaagagagc taatgtaact aatagaatgg
     6001 aaggacataa gtggaatagc ttatactaat attaaaataa gattgtaata atattctatt
     6061 acaacattag tttatctgta ggaggagaaa atggaaaaaa taaaattaaa tattaatgat
     6121 tacatgtatg aaatagaagt tgaaaagaat gaaactcttt tgcatgtact tcgtgaaaaa
     6181 ttaaatctaa ctggagcgaa acaaggttgt aatagtggca gttgtggagc atgtaaagtt
     6241 ttgatagatg gagttgacat gaaatcttgt aaacttcttg caaaaaactg cgaaggtaaa
     6301 gttatataca ctatagaaag ttttgctaat ggagaaaatc ttcatccaat acaagaagct
     6361 tttatagaag ttggagctgt acaatgtgga tattgtactc ctggtatgat tataacaaca
     6421 caagctcttt taaatagaaa tgcatctcct aatgaagatg aaattagaga agcttttaaa
     6481 gaaaatctct gtagatgcac aggatatgta aagattatag aagctgtaaa attatctgca
     6541 gctagattag ggggtagata attatggaaa aggtaatagg aaaatcatac cctctaaaag
     6601 atgcagcttt aaaagttact ggtcaaatga aatacgtggc tgatatgaaa ccacaaaatg
     6661 tactttacgc taagatgctt ttaagtcctg tagcacatgc aaaaataaaa agtatagata
     6721 caagtgaagc tgagaaagta gtaggtgtta gagctatagc tacttatctc aatacatcac
     6781 aaagaaaata caatggttca ttaagatttt atgagcacaa tatacctgaa aatgaagtta
     6841 tattttcaga tacagttaga tttgttggtg accgtgttgc agcagtagct gcagagactc
     6901 cagaaatagc agagaaagct gtaaaactta taaaagttga atatgaagaa ttgccagctg
     6961 tatttactat acaagatgca ataaaagaag atgctatctg catacatgga gattcaaata
     7021 tagtttctca aaacaacata aatgggggag atgtagaaaa aggcttcaag gaatgtgact
     7081 atatatttga agatgaatac tctactccag caatacatca tagtgcaata gagactcata
     7141 gtgtaattgc acactatgat tataatgata aattaactgt acatacacct tgccaaaata
     7201 catttggatt tagaataata ctatctcaaa tttttgggat gtcattaaat aaagtaagag
     7261 ttataagacc tgctattgga ggctcatttg gtggaaaatt agaacttaca attgaaccag
     7321 tagcagccgc attatcaaaa atgacaagaa gaccagttaa aatggttctt acaagaaaag
     7381 aatctatgat atcgacaaga actcgtcatg ggtcttttgt aaaattaaaa actggtgtaa
     7441 ataaagatgg tgaagtaata gcacaagata taaaaatata tacaaataca ggagcatatg
     7501 catcaagtgc tttaaatgtt ataggagcac ttagtcataa agtatttaaa gtttataaaa
     7561 taccaaatat aaagtttact gggatgcctg tatatacaaa tacaccaata gcaggagcta
     7621 tgagaggcta tgggtcacct caaatattta tggctcaaca agctcaattt gcgaaaatag
     7681 caaaggaaat aggtatggat ttagtcgatt ttcaaaataa aaatgcagta gagccagatg
     7741 atgtcgatat aatcttccac ggttctcttg gaaatcctag agtattggat tgtatagagc
     7801 aaggtaagaa gatgtttaag tgggatgaaa agaaaaaact tccaaaagaa gaaggaaaat
     7861 atttaagagg tataggtatg gcaataggtg cacatggtaa tggagttttt ggtgcacata
     7921 gagatgttgt tacattgact cttaaattaa atgaagatgg tacgttgaca ctactaacag
     7981 gagctcatga tatgggcaat ggtgttgtaa ctatgcaaac aatgatggta gctgaagttt
     8041 taggaataac acctgataaa gttgatacat ttgaaactga tacggatgca tgtacattca
     8101 accttggtga ctatgcaagt agaggggtat ttgtagaagg tggaggagct aaaaagacag
     8161 cagagaaatt gaaaaatctt atattagaag aagcagaaaa attacttgaa acatcaaaag
     8221 aagatttata tctagacaat ggatgtgtaa ttagtaaact tgatgaaaat gtaaaagcat
     8281 cactttctga tgtcgcagta tatagtcaaa gtaaatcatt aagagagtta actgttgttg
     8341 aagattattc ttcaccagct ggacgtactt cttatggtgt acattttgct gaagttttag
     8401 tagataaaga aactaaggat ataaaagtag ttgattttgt agctgtacat gatgttggta
     8461 gagttataaa tcctctaaat ttagaaggac aattagaagg tgggattcat atgggtctag
     8521 gatacgcatt gagtgaagaa ttggcatttg atgaacaagg tagacctaaa gctactgatt
     8581 ttaaaacata taaattattt agaactgtag atatgccaaa atgtcaagtt gcatttgtag
     8641 atggttatga aaaagctggg ccttttggtg gtaagagtat tggagaatgt gccgttgtac
     8701 cagttgcgcc agctgttgca aatgcagtat ataatgcaac tgatgtagag atacattcat
     8761 taccaataaa attaaaatag tagaaattaa agcagaaata acaatttatt gtatttgtaa
     8821 atagataata aaaatagatt tatttggtga ggagatatta ttatggcaaa gatattaggt
     8881 atttgtggaa gtccacgtaa aggagcaaca gaatatgcag tgttagaagc tttgaaagaa
     8941 gcagaaacta tacctggtat agaaactgag tattggtcag ttagagggaa aaaaataagt
     9001 ccttgtgtac attgtgatgc atgtataaga aaagaaacta tgtgtattat aaaagatgat
     9061 atccaagaat tagagcaaaa aatattggag gcagatggaa ttatagtagg ttctccagtt
     9121 tatgatatga atataactgc tcaacttaca gcagtattta atagacttcg tccaatgtat
     9181 ttagttcatc caggaaaact tcaaaataag gttggtgcag ctataactac tggtggaact
     9241 agatatggtg gccaagaatt gacaaagctt cctataataa atttcttcct aatgcatgaa
     9301 atgttagtat ctggaggttt aggtggatgc tatataggtg gtacaatatg gagtaaagat
     9361 ggaaaagcaa aaggtgccca agaggatgag acaggtatgg acacagtaaa aagacttgga
     9421 aaaggtgcag cagaggcagt tatggtttct aagtttggtc ttgaaaaatg gaatgtcgta
     9481 aaagaagaat tagatgttaa aaaagatgaa aaatcacctc ttagagacca ctaaagattt
     9541 attatattat tgaatatata caaatagtga tgtaatagat tattgcatca ctattttatg
     9601 ttttaattta gattttatag attttatatt aaaaaatata gaaacaaagt gttttgagat
     9661 ttatttttaa attttaaaaa tgagaattaa aaagtgagaa gatagaaaag gttttttagt
     9721 tattaaattc tacatacatt gtatgaacta aagatagaaa agaaaaaata ataaaagcat
     9781 tgcaatttct atactaaaga gtcatggaaa aatatctagt atatatttag tattggcatg
     9841 ttaattgctt atatattaaa gtataaaaat ttaacaataa tttggaggta ttttagatgt
     9901 taaaagaaat taaatggaaa gtaaataatt tgccaaaggg cgataaggaa aattgtatca
     9961 aatttttaaa tgaagaagaa ataactaagg ttagaaattt tcacaaaagc tttcctcaat
    10021 ataaagaaac tccattagca aatctagaag gtttagcaaa aaaattaggt gttgcaggag
    10081 tatatgtaaa agatgaatct tatagatttg gattaaatgc atttaaagta ttaggtggtt
    10141 cttattctat gggtagatac ttagctcaaa gattagatac agatatatct gagttagggt
    10201 atgacaaatt gacttcgaaa gaaataaaag aaaaattagg agaaataact ttctttacag
    10261 ctacagatgg aaatcacggt agaggagttg cttggacagc taataaatta ggtcaaaaat
    10321 ctgttgtatt aatgccaaaa ggttcttctg aatttagatt aaataaaatt aaaggagaag
    10381 gtgcagatgc tagtataaca gacttaaact atgatgatgc agtaagatta gctaatgatt
    10441 atgcagaagc tgatgaccat ggagtaatgg ttcaagatac agcatgggac ggatatgaag
    10501 aaataccagc gtggataatg caaggatatg gaactatggc tcaagaagct atagagcaat
    10561 taaaagaata tggagtagat agaccaacac acgtatttgt tcaagcagga gttggttcac
    10621 ttgctggtgc agttcaaggg tatgttgctt caatatatga tgaatgccca ataacagtag
    10681 tagtagaggc tgatgaagct gattgttact ataaatcagc agaagctgga gatggaaaac
    10741 caagatttgt tggaggagat atgccaacta taatggcagg gcttgcttgt ggagagccaa
    10801 atactatagg atttgaagta ttaaaaaatc atgcagcagc atttgtttca gcaccagact
    10861 gggtttctgc taaaggtatg agaacattag gtaatccttt aaatggtgat gaaaaagtaa
    10921 tttctggaga atctggagca gttacaactg gtcttttagt agcagctatg gaaagagaag
    10981 atttagcaga tttaagaaaa gatttaaaat tagatgaaaa ttcaagaata cttttaataa
    11041 gcacagaagg agatactgac cctgacaaat atagaagtat agtttgggat ggagaatatc
    11101 caagtatata aggaattact aaaatgcaat tattagatat tggtttacat atttagaaga
    11161 tatataagtt ctaaaattta gaaaatatat aagtatataa aatacataca ttataaaaaa
    11221 tctaggggga taatgaaatg ttaaacgaac aaagaaaaca agaagtaact gaaatatgcc
    11281 aaaaattaat tcaaaatcct agttcttctg gaaatgaaga aggtgttgta aaagcaatag
    11341 aagagtcttt tgaaaaatta ggttttgata gttggtcaag agatagatat ggaaatatag
    11401 taggatgtat aaaaggtaac aagccaggta agaaaatatt atttgatgga catatagata
    11461 cagttccagt tccagatgca agtaaatgga gtgttcctcc ttttgaagga aaaatagttg
    11521 atggaaagat atatggtaga ggaacttctg acatgaaagg tcaatatact gcaatgatgt
    11581 ctgctgtagc gtattttgct aaagacacta atagagattt tgctggagaa ctatatgtag
    11641 caggtgttgt tcatgaagaa atatttgaag gtgtttcggc tagagagata agtaaggctg
    11701 ttcaacctga ctatgttgta ataggtgagt cttcagagtt aaacttaaag attggtcaga
    11761 gaggtagagg agaaatagta gtagaaactt ttggtaaacc agctcactct gctaatccag
    11821 aaaaaggt
//
LOCUS       DAEMHZ010000125        11714 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000125 DAEMHZ010000000
VERSION     DAEMHZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.125"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..749
                     /locus_tag="KPK72_003028"
     CDS             120..749
                     /locus_tag="KPK72_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490222.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            750..1532
                     /locus_tag="KPK72_003029"
     CDS             750..1532
                     /locus_tag="KPK72_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490223.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1525..2403
                     /locus_tag="KPK72_003030"
     CDS             1525..2403
                     /locus_tag="KPK72_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490224.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIVNDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2436..3395
                     /locus_tag="KPK72_003031"
     CDS             2436..3395
                     /locus_tag="KPK72_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2490225.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3710..4402
                     /locus_tag="KPK72_003032"
     CDS             3710..4402
                     /locus_tag="KPK72_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490226.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4412..5416
                     /locus_tag="KPK72_003033"
     CDS             4412..5416
                     /locus_tag="KPK72_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2490227.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5684..6535
                     /locus_tag="KPK72_003034"
     CDS             5684..6535
                     /locus_tag="KPK72_003034"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2490228.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6639..7385
                     /locus_tag="KPK72_003035"
     CDS             6639..7385
                     /locus_tag="KPK72_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490229.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7385..9301
                     /locus_tag="KPK72_003036"
     CDS             7385..9301
                     /locus_tag="KPK72_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490230.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     GVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9580..9813
                     /locus_tag="KPK72_003037"
     CDS             9580..9813
                     /locus_tag="KPK72_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG2490231.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            10009..10437
                     /locus_tag="KPK72_003038"
     CDS             10009..10437
                     /locus_tag="KPK72_003038"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG2490232.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKRFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(10892..11671)
                     /locus_tag="KPK72_003039"
     CDS             complement(10892..11671)
                     /locus_tag="KPK72_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490233.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEISLSQREHFKI"
BASE COUNT         4596 a         1126 c         1954 g         4038 t
ORIGIN      
        1 ttaaaaaatg tagataaaaa ttgttttatt aagaaaaaat taatatattg acaaaattta
       61 agtgctcatt taaactatta attgataata atattcaata ttaaataata ggaggcgtta
      121 tgaataaata tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc
      181 ttattaaagc aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag
      241 aatatgtaaa aaagcagtac aaagagtata caaaaaagac taaaaataca atgttggatt
      301 gtagatgtcc agaaagtata agtcttttaa aaagaaataa tttgataaat gattttgaag
      361 tacctatgat agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa
      421 aaagtaaaga tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa
      481 gtgaaaagtt taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac
      541 aagaaggtgt aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca
      601 aagatacttt tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac
      661 aaaaaataag aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag
      721 atggttgtaa taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa
      781 ggctagtaaa gagtgttttg atatttttta taattctttt attatggaaa attacaaatt
      841 atcttggtat ttggagtgat tatatattac catcacctga aaaggtatat agtacatttt
      901 taaatatgat aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt
      961 tgataggttt tgcaataagt actgctatag gtgtgccttt agggatattc tttggaattt
     1021 atagtggagt atatgaatac tttaaatctc taataaattt tttaaggaat acacctcccc
     1081 ttgctttaat accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa
     1141 ttatagtttt agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt
     1201 gtgactcaaa actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat
     1261 ttaaaataat aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg
     1321 gatatagctt tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat
     1381 acttgatatc tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg
     1441 taattggact tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa
     1501 gtaagggaaa gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt
     1561 agaaatttat ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc
     1621 tctttaaaca tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag
     1681 actaccttgc ttagagcaat tgcatcactt gaaggtataa atagtggtga aattaggttt
     1741 attaatgata ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta
     1801 atgccttggt taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt
     1861 ttaaacaagg tgcttagaaa atttaaacac aataaaagaa ttagcagtaa aattgtaaat
     1921 gataatttta gaagcttaga aacaaacgat aatttaaaca aatcaaataa atataatagg
     1981 aaaagtaata ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac
     2041 tcttatccaa atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt
     2101 tctttcaatc cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca
     2161 aggattgata tgcaaaatga agttataaga atacataagt caactaataa aggcgtgata
     2221 tttgtaactc atgatattga tgaagcgtta aagataggca agaaaatagt tgttttcaca
     2281 gacgaaagaa aagtaaaaga atttaatata gaagatggtt ataatagaga tttaacttca
     2341 gattactata ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaaggcatt
     2401 taaagaaatt aataatattt taaggagtaa agtaaatgaa attaaaaaag ctattgatag
     2461 gtataacgat attgacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg
     2521 gagaaaaact ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta
     2581 taattcagaa acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta
     2641 acttccacga aattactaca ggtccagagc aaacacaagc tttagctgct ggagaaatag
     2701 acttcttaca tgcactaggt ggtacatcag ctttaatagc tgcatctaat ggagtagagt
     2761 taaagatatt aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata
     2821 attcaataaa atcagcagca gatttagtag gtaagaaggt ggcaggccca aaagggacta
     2881 tattacatca ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg
     2941 aatttgttaa tatgggtatt ccagaggcat cagcagctct ttctgatggc agcgtagatg
     3001 ctgcattgat tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag
     3061 caaatggtga aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag
     3121 agaaacatcc agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg
     3181 taaataataa ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg
     3241 aagaaactaa agaattatat gcttggtatg attttagttt agatataaca gataaagaca
     3301 tatcttcgct tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag
     3361 tgaacataaa agaattaatc tataatgcta agtagaaact atgataaaat aatactaagt
     3421 aagaaatata ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag
     3481 taatttcaat ttattttaga atgattagtt gataataatg attaattaat cataaaaaga
     3541 tactttttta tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt
     3601 aattgtgaag ttagtaatat gattaaattt atatattgta aaaagattat aatatattaa
     3661 aattggactg ttaatatgat gagtaaatag gtatattagg aggaattata tgagttataa
     3721 agtatatatt gtggaagatg atatatcaat aagtacatta cttaaagatt atattgagaa
     3781 atatggattt gaagtgattg ttgaagaaaa ctttgatgat ataatgatta catttgaaag
     3841 ttttaagcca cacttagttc tattagatgt aaatttacct aaatttgatg gtttctactg
     3901 gtgtagatgt attaggcaac agtcgaattt acctataatt tttatatcag ctagagatgg
     3961 taatgtagac caaattagag ctttagaaag tggagcagat gattatataa caaaaccttt
     4021 ttactatgat gtagtaatgg caaaaataaa gagtcatata agaagggtat atggtgaata
     4081 ttcacctaaa atagacgaaa gaatagtgga gctagatgga ctaaagtttt atccagaaag
     4141 gttagaacta gaattaaatg gtgagaatct aattataact aaaaaagaag gtatactgat
     4201 tgaatgtctt atcaagagat acccaaaagt agtaagtaga gattatcttt tagaaaagat
     4261 atgggatgat attgagtttg tagaggaaaa tactttaaat gtaaatgtta gcaggataag
     4321 gaaaagattt gaggaattag gtatagataa tgctgttcag actgtccgag ggttaggata
     4381 taaattaaat aagaattggt aaggagaaag tatgaagtta tttttaaaag attataaagg
     4441 ttatattttt atatattata taggcatagt tatgacaatt atttactgta atcttatgaa
     4501 gtttattagt tttggagaga gtttatatat tcttttattt agttctttta tattgttatg
     4561 ttttttaata tataagtatt ataaaaatag agaagtatat aatctgtttg aaaaaggagt
     4621 acattctttg gaggagtcat ttttgtattt aggaagctca ttcttgggga gaaatatatc
     4681 agttatttta aaacaacaac ataaattgta tcaatcaatg atacaagagc ataaaaagat
     4741 acatagtgaa catttaactt tcatcaacca atggattcat cagatgaaaa caccaatatc
     4801 agttataagc cttcagcttc aagattatga gggagaagaa gtaactgaaa atataagaaa
     4861 agaaatagat agattaaata aagggttaga tatggctatg cattttgcaa gggcagataa
     4921 ttttcagaaa gactttatag ttgaaaaagc caaattaaat gaaattgtat ctagtattgt
     4981 aaatgaagaa aaaaggttat ttataaaaag tggaatgatt cctaagattc aagtagacaa
     5041 gtttacttat gtttatacag atgtaaaatg gatgaaattt ataataggtc aattaattac
     5101 aaatggagtg aaatattcta aagattatag taactaccta actataaaat ctgaagaaaa
     5161 taataaatat gtaattttaa gtataataga tgaaggaatt ggaattgttt ctaaagattt
     5221 aaaaagggta tttgacccat tttttacagg agaaaatggt cgtaagtttg gagaatctac
     5281 aggtatgggt ctttatctag taaaaaaggt atgtgatgat ttaggtcata aagtaagtat
     5341 aaaatcagaa gtaggaaaag gtacagaagt ttcaatctac tttaataaag atgtaaatag
     5401 tttaataaag gtgtaaatag tttgataaag atgtaaatta tctgttacaa tagggttttt
     5461 agttaattgt acttttttat tatacaattt attaaatgaa atattcatag ttaaataatc
     5521 aaaatttaag agagtttatt tgcaaacctt acatagatgt aaggtttgtg taatgtttgc
     5581 agatagttaa attttctaat ctaaattaca attaatataa gaaataacat aagagaaata
     5641 acataaaaaa agaaattttg taaaaaagga aggatgttta ttgatgagtt tagatgttat
     5701 ttttatattg aaatcagtca taatagctat agtagagggg cttacagaat ttattccagt
     5761 ttcatctaca ggtcatatga ttttggttgg taatttaata gattttaagg ggcaatttgc
     5821 tgaaatgttt gaggttgtaa tacaacttgg ggctatatta gcagtagtag tactttattg
     5881 gaaaaagata aaagatagcg ttatagaatt ttttaaattt atatttactg gtggaaaaga
     5941 aggaaagatt ggttttagat ttggaatgaa tgtaattata ggttgtatac catttgcgat
     6001 tataggagtt ctattttacg ataatataaa atcattgttt aatttacaat ctgtaattat
     6061 aggatttata gtgggtggga ttttattatt agtagtagaa actttgttta gaaaaaagaa
     6121 tcattccaca gataatatag ataaaattac tccaatacaa gctttaaagg ttgggacatt
     6181 acaagttttg tctgcatggc caggtatgtc aaggagtgca tctactatta tgggtggatg
     6241 gatagcagga cttaattctc ctacagcagc tgagttttct ttctttttag cagtaccagc
     6301 tatggtggct tcttcaggaa aagatttatt tgaatttgat tattcaataa tgacaccaac
     6361 attgtggatt gctttggtag ttgggtttat agtagctttt attgtatcaa taatagttat
     6421 ggaaaaattc gtgaattttt taaagaaaaa accaatgaga gtgtttgctg tatacagaat
     6481 aatcatgggt gtagtacttg cagtattagc ttttactaac ataattagtg tatagaaaaa
     6541 atagatttat tatctataaa aattaaacta agataaatta taattaacaa ttatatatat
     6601 gttatttcaa aaattaaatt aggaaggata gttaaataat gaaaatttta actgtaaata
     6661 atttaagtaa agtatatggt aaaaaaataa ttttcaacgc attaaatgac ataaatttta
     6721 gtatagaaga tggagaattt gtaggaatta tgggtccatc tggtagtgga aaaactactc
     6781 ttttaaatat gatttctact attgacaagc caactacagg aactatggaa ttaaagggca
     6841 agaatccttt attgttaaga ggtgaggaac ttgcattatt tagaagaaga gagttaggtt
     6901 ttgtatttca ggattttaat ttattggata cattaactat aggagaaaat atagtattgc
     6961 cattgacatt agataaggtc agtgtaaaag agcaagatga gagattgaat gaagtatcta
     7021 caatattagg tataaaagat ttattaggaa aaagaacttt tgaagtctca ggtggtcaag
     7081 ctcagaggac tgctattgct agagcattga ttaacaatcc atcaatactt ttagcagatg
     7141 aaccaacagg taatttagat tcaaagtcat caaaggttgt aatggagcta tttcaaaaga
     7201 taaataaaga aaataaagtt actactatga tggtaactca tgacccatta gcagcgagct
     7261 actgtagtag aatcttattc ataaaagatg ggtctatata taatgaaata tataagggta
     7321 gtagtagaga gcagttttat caagagataa tggatgtact cacattattg ggaggcgata
     7381 actaatgaat ataaaacaat ttgctattaa caatatttca agaaatatta aagcgtattt
     7441 aggatactta gcaagtattg ttatatcatc ttcactgcta ttttctttta ttatgtttac
     7501 aagtcatcca gatttaggtg tatctcaatt tcctgattat ttaaaagaag gtctcaagat
     7561 gagtaagata attgcatatc tattcttatt cttctttgtg ttttattcag ttagtgtgtt
     7621 tcttaaaagt agatataaag aatttggaat actttatatt acaggtatat caaaacgcca
     7681 agtaatgaaa ttaatcttta tagaaaatat gataataaat atagtatctt ctgtaattgg
     7741 aataataata ggcttaatct ttgcaaagat atttttagtg gttatgtcta cttttttaga
     7801 attatcacca ttaaaattct atataccatt taatgcgata ttgtctactt taatctattt
     7861 tatgatttta gctgtattaa catcaatatt tacttctttt atagttaaag aaaatcaggt
     7921 gttgaagtta cttaaaggta caaaaactcc aaaaccagag cctaaaacat cactaatgct
     7981 tgctatatta agtatttgtt tgtttataat agcgtattat aatgctatta cctcaaccga
     8041 ccaatataca acatatctgc gcttagttcc agttacatcc cttactatag tagcaactta
     8101 cttgttcttt tctcaattta gtgtattttt tatgaagaag ctaaaactaa acaagagatt
     8161 ttatagaaaa aatacaaata tgctttggat ttcgaacttg atttataagg taaaggataa
     8221 tgctagaata tttttcttga taactataac ttctgcagtg gcatttactg ctataggtac
     8281 agtttattcc ttttggaaag atgtagagcg tcaaattaat ttaatatatc ctaacacaat
     8341 atattattca acaatgacat tgcataatga ccctaaaaag cctgatagca aagaaaaaga
     8401 caaggaaaga atgatgttta tagaaaataa tctaaaaaaa gagaatgtag actatacaag
     8461 gactaatggt gaatttaaaa ctgtatttcc caagaaaagt gattttaatg tcagaattat
     8521 gaaagaatct aagtacttag aaattacaga aactataggg gttaagccta taagttttga
     8581 agataatgaa tgtatatcat tattatcatc aagtttacca ggaaataata acgtcaaaga
     8641 aaatattatt gtagggaata gaggtttaaa ggtggcaaaa caagtggaag aatgtgttat
     8701 gccagcgtat tacaaaaata tgtatgtggt aaaggataat ttttatgata gtatagaatc
     8761 aaaatttata gtggatagat ttactgcatt tgaagtagag gattcaagta aaactataga
     8821 gatatgcaga gaatttgaaa ataaatttaa taatgagtca gggatgcaac catatttgtt
     8881 tttctctaaa gaattaatga taggaactgg gaaattaatg tattctacat ttatattttt
     8941 agcaatattt ataggtttaa ttttctttgt tactacaagt agttttttat ataataaatt
     9001 ttatatggat tgccaagttg ataagaaaaa gtatgagcaa ttaaataaat tggggatgac
     9061 atataatgag attaaaaaag cttcaacaat tgagattgga atagtatttt tgtttcctta
     9121 tgtagtggct gttatacatt caatatttgc acttactgca cttaaaaatt cttttgatat
     9181 agaagtagca tcatcagctg ttttggtaat gggaagcttg ttcttggttc aaatagttta
     9241 ttttttaatt gtaagaaata attatttgaa agagataagg ttaaatttag tcaattttta
     9301 gttactatta ttaaatgata ataaaaaggg tttaaaatta attaatatta ctggaattat
     9361 agatgtttat tttttcagta atatttttta ttgtattaac tattaaaatt ttgtacaaat
     9421 ataaaatata atatggtaaa tgtcaaaatt ataaaaaaat tctacttata taaaatataa
     9481 agaggttata tgtacatatg tgtagaaata aataataaaa atatcaatta atgttattag
     9541 ggggggttac tcattagtaa attagctaat acacttagaa tgatgatatt aattcaaagc
     9601 agaggcaaaa tgaaatgcaa agaattatca gaagagttgg aagtaagtga aaggcaaata
     9661 aaaagttaca agatgtattt agagcaagca ggtatattta taaattcaac acctgggatt
     9721 tatggtggtt atgaaataga taaatgtaat agcatctcat taattaaatt attagatagt
     9781 gaagtttcaa tttttatatc aactaagtgg tagataagta gaatattagc aatatcaacg
     9841 agtatttaga aacttatttt atattaataa agcagtatat aaaattgtag aatcaacaat
     9901 agcatatact aaaacatcca ccttatatta actaaatcat atctaaataa aatacactta
     9961 cctacaaaca ttataaaatc aatacaaaaa tgaggtgaaa caaaatttat gataaagaaa
    10021 ttaaacagta aagatataaa taaaatcatg gaaatatggg aaaaaagtac aatcaaagca
    10081 catgacttta taagtaaaga atactggcaa aataactaca atactgttaa aaacgaatat
    10141 atacctatat cagatacatt tgtatatgat gatggagatg aaataaaagg atttataagc
    10201 ataatagata aaagatttat aggagcttta tttataaagc ccaaatacca aaatctaggt
    10261 atcggaggta aacttttaga ttatgcaact aaaaaatata aaagtctaag cttagcagta
    10321 tataaagata ataaaaaagc agttgtgttt tataataaaa aaggttttaa tatagtaaaa
    10381 gaacaagtaa atgaagattc aggatttaaa gagtacataa tggaatatag taaataatat
    10441 gattacattc tattaaaagg ataattaatt tatttgccat ttgatttttt aagagtaatt
    10501 tttaaaatag actatatgaa gataaagata tttattatag gtgtaagtta atcagaattg
    10561 atatagctac aaaagaggaa aataaaattg tatggtgtat ttcagattta acaaatttac
    10621 tatttaaaga taataagttt gatataatat taaagatact ttcacctata aactataggg
    10681 tatttgaaag aattttaaat aattatggaa tgctaataaa ggtttgttaa aatttaataa
    10741 ttattagatt atagaaaatt gccatgaaat tagttcatca agaactagtt tcatagcaat
    10801 tttaattctt ataagtgtat ttatttttac agatttaaaa aacttataca gtatctaata
    10861 atgtaatctt attactttag cctataaata tttaaatctt aaaatgttct ctttgagata
    10921 agcttatttc agagtcacaa tttccacaac agtaagatgt tgagacatct ttaaattttt
    10981 cagtattctc atcaaagttc atttttttct ttgcaataat tcctacctta gttaaattag
    11041 aaccacaatt attacagttt tgaattttgc ataggttata tagtttatca agtgtaatat
    11101 cttctaataa ttcatcacaa ttacaacatc tgatagaaga aacttcacta cacatttcaa
    11161 taggttcagc ttctttagaa gaaaatttat atgttatgtc atttgatact ttataacctg
    11221 tatcaaatat attagaatta caacttggac ataagtattt tggaagatat atatcttttt
    11281 tttcaaaagt atcactacta atattacagt ttaattcatc taaaattggt ctttcatcta
    11341 ggttattacc tttatttaag cttagaagat aattataaat ttttttatct tcattctgtt
    11401 ttgatgtatc taacaaatgc tccattatat tagtgtattt tttgtttttt gaaggcatgt
    11461 ttaaatccag ataattgttt tcaatagcaa agcatactat attgaaatag ttatatttaa
    11521 ctgcaaaaaa taataacatt tcatcaaata ctttatctat tattttgtca gaaatactta
    11581 tccaaaaatc tggtgactct aagtaaactt tcctactaag ttcatctaac tcttcatcta
    11641 tattatttat attattttca ttaactttca tatcatcact tccttttaaa tatcttgtgt
    11701 ataaactatt tgtt
//
LOCUS       DAEMHZ010000126        11712 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000126 DAEMHZ010000000
VERSION     DAEMHZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.126"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            80..535
                     /locus_tag="KPK72_003040"
     CDS             80..535
                     /locus_tag="KPK72_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2490234.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSSLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            584..1168
                     /locus_tag="KPK72_003041"
     CDS             584..1168
                     /locus_tag="KPK72_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG2490235.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1173..1496)
                     /locus_tag="KPK72_003042"
     CDS             complement(1173..1496)
                     /locus_tag="KPK72_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490236.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1648..2166
                     /locus_tag="KPK72_003043"
     CDS             1648..2166
                     /locus_tag="KPK72_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2490237.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2343..3011)
                     /locus_tag="KPK72_003044"
     CDS             complement(2343..3011)
                     /locus_tag="KPK72_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490238.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3126..4541
                     /locus_tag="KPK72_003045"
     CDS             3126..4541
                     /locus_tag="KPK72_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2490239.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAGFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4764..5222
                     /locus_tag="KPK72_003046"
     CDS             4764..5222
                     /locus_tag="KPK72_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490240.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5219..5803
                     /gene="clpP"
                     /locus_tag="KPK72_003047"
     CDS             5219..5803
                     /gene="clpP"
                     /locus_tag="KPK72_003047"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2490241.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6115..7191
                     /locus_tag="KPK72_003048"
     CDS             6115..7191
                     /locus_tag="KPK72_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2490242.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7258..9243
                     /gene="bclA3"
                     /locus_tag="KPK72_003049"
     CDS             7258..9243
                     /gene="bclA3"
                     /locus_tag="KPK72_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG2490243.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGADGATGVTGPTGATGATGANGLVGPTGATGAAGTPG
                     ATGPTGATGPTGVGITGATGATGPTGADGATGPTGATGNTGADGVAGPTGATGATGNT
                     GADGATGPTGATGNTGADGATGPTGATGATGVAGATGATGPTGATGATGADGATGPTG
                     ATGATGADGATGPTGATGVTGVTGATGPTGATGATGATGASAIIPFASGIPLSLTTIA
                     GGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFSTT
                     AALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLN
                     IAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9412..10173
                     /locus_tag="KPK72_003050"
     CDS             9412..10173
                     /locus_tag="KPK72_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2490244.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10482..10760
                     /locus_tag="KPK72_003051"
     CDS             10482..10760
                     /locus_tag="KPK72_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490245.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11053..11628
                     /locus_tag="KPK72_003052"
     CDS             11053..11628
                     /locus_tag="KPK72_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG2490246.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         4471 a         1474 c         2164 g         3603 t
ORIGIN      
        1 tattgaaatg attaaattta tttacttaat gtaaattatt agctaaaatt gtattaaata
       61 attaaggagg tgagtagctc tggatgaaat agatttaaaa ataattaatt ctctaaaaaa
      121 gaatagtagg gcttcaacct ctgaaataag cagacaaatt aatttatcag taccagcagt
      181 ctcagagcgt ataaggaaaa ttgagcagtc aaatttaatt caaaagtaca ctattcaaat
      241 taatcgggaa aaaacaaaat ataagctttt ggcttttata tttatcaata ctgatttatc
      301 taaaagtatg aaagaattta agaaaactat attggaatat gatactgtat tagaatgtca
      361 tcatatttca ggagagtatg attacttaat aaaagtatta gcagaagata caagtagtct
      421 tgaagagttt atatcagtga ctttgaagga aattgggggg attcaaaaag ctaatacaac
      481 cattgtttta tctacaatta aagaagaatt taatacaata aaagatattt tttaaataat
      541 ttactagtaa agtgttaatt atattttagg aggaaaatag aatatgaaag ttttagtttt
      601 tttagcaaaa ggttttgaaa ctatggaatt tagtgtattt gtagatgtta tgggatgggc
      661 tcgtaacgat tatgggcatg atattgatgt agtgacttgt ggatttaaaa aacaagttat
      721 gagtacattt aatatacaag tattagttga taagacgata gaagaagtat gtgttgatga
      781 ttatgatgct ctagctattc ctggaggatt tgaagaattt ggattttatg atgaagcata
      841 tgattcatct tttttaaatc taataaggga gtttaattca aaagaaaaaa taatcgcttc
      901 tatttgtgtg gcagcattgc cagttggaaa aagtggtgtt ttaaaaaatc gtaaagctac
      961 tacatatcat ttgaaaaatg gtaaaagaca aagacagtta agtgagtttg atgtaaatgt
     1021 agttaatgaa ccaattgttg tagataaaaa tattataacc tcatattgtc cagaaacagc
     1081 tccacatgta gcttttaagc ttttagaaat gttgacatct aaagaacaaa tggatgaagt
     1141 taaattagca atgggattta aactgtaatg gtttatagat tttctaagta atcagaaccc
     1201 cagtcacaca tggaatctaa aattggaata atactctttc caaaatcaga tagcgagtat
     1261 tctacttttg gaggaacaac aggataaacc actcttataa ttaaaccatc tgattctaat
     1321 tcacgtaatt gttgagttaa catcttaggc gttgctttag gaattaattt ttttaattca
     1381 ttgaacctca atatagtgtc tttaagatgc caaagtataa gtgtcttata tttaccacca
     1441 atcaatgcaa gtgttgcttc tattgggcaa ctgtaattat ctaaacattt tttcattttt
     1501 gggctccttt atttatttta aagtatcttt ttaggtacta tattacaaaa aagtgcatta
     1561 ttgtaatata gtaccatagt gataaaatta tagcataggt taaataaatc atcaacttag
     1621 cctaataaaa caataggagg taaataaatg aatttcgttg aacttgcaaa aaaaagatat
     1681 tcttgccgta attatcaaga tagaaaggtt gaaaaagaaa aacttgagaa ggttttagat
     1741 gttgcaagaa tagctccaac tggtggtaat cgtcaacctc aaagattgat tgttattcag
     1801 gaaaaagaag gtataaataa actttctaag gctgccaata tatatgatgc gccattagca
     1861 attcttgtat gtggagataa agacaaagtt tggacaagac cttttgatgg gaaacaactt
     1921 acagatattg acacaagtat agttacagac catatgatgt tacaagcaac tgaacttgga
     1981 ttggcaagtg tttgggtgtg ctattttaat ccagatataa ttagagaaga gtttagtttg
     2041 ccagataatc tagaaccaat aaatatatta ttgatgggat atgaatctaa gataccagag
     2101 agcccagaga gacatgaaaa aactcgtgtt cctttaagtg aaattgtttc ttatgaaact
     2161 ctttaattat aaaattaagt tattcattag gataaatgta ataaagtgtt tattataaac
     2221 aaaattcaaa ataaataata tctacatata attaatttta gtgaagtata tctagtttaa
     2281 ctagaatgta tacttcacta atttatttat tcaaaatatt tatgaggatt ttaatatatt
     2341 aactaaaata tatcaattga attaactttt aaatctggaa aagcacttga aatatagtgt
     2401 tttatgtcat ttaaatggtc ttgaatttgc ttttctgata ttttatcagg tccatataca
     2461 aaatacataa tatttttagt atcttttgtt atagctgttc gattatcagg gccattaaga
     2521 atactagata atataccaat attatctgat aaaaataaat catcttttgt taaaatatgt
     2581 tctttaccac ctataccata aaaatgttca cttccatttg ctaattctat tttcaaaggt
     2641 aattccattt tatcaaggtc atgacctgct gttagaagaa gatttttcaa ttctgctaaa
     2701 aacattgact caattgttac acctacatct gggatacctt ttgattttaa tagaatagat
     2761 tctaattgaa gcaatacagg gtaagttttc ttgaattttt tataatactt tatataactt
     2821 gaaacaggct ctgttttaat tttttcttta cgattgtatt ctgggtgttg ttctataata
     2881 tttttaattg tagagttttt aagaactaaa aaattttctt tattaggttt tgaatgtgta
     2941 atatttataa ccataactcc aaatttaata ttgggataaa tttctttaac tttatgtgat
     3001 atttctaaca taatatagac tcctttttat tttatatttt atggtataat cttagcaaat
     3061 taaaagtgga ttttgtagtt ccatttttat agtaatttta tagaaccact tttggaggta
     3121 ttagaatgtg gattacgatt aatagaaata gtaatatatc attatcaaga caaatttatt
     3181 gtgagataaa agatatgatt ctggatggaa gcttaaagtt tgagcaaaaa ctgccatcat
     3241 ctaggacttt atcaaaagaa ctatctgtat ctcgtaatac agttttagat gcatataatc
     3301 aattaattgc agaaggatat ttggaaacaa ggaatggata tggaacaatt gtagctaaag
     3361 gaatatcagg gcataaagtt gattttaagt taaatgatac ttttagagat gaaaaagatt
     3421 tacatactaa taaacaaaaa aattatatag attttcgttc aggagttcca gaattaaaac
     3481 tatttccacg caaagaactt tcaaagctat ataaacaaat actgtatgat ttatcagatt
     3541 cggatttcag atatggaagt acagctggtg tatgggtttt aagagtggaa atatctaagt
     3601 atttatttcg tactagagga attagatgta atcccaaaaa cattatgatt gtttctggtt
     3661 caacacaagg tttatcttta atttcacagc ttttatacaa aagtaataaa aaggttgctg
     3721 tagaagaccc tattcataaa ggcttattaa aggttatttc atctattgga tattcagttg
     3781 ttggaattaa agtagatgat aagggaatga ataccaactt aataaaatca ataaagggga
     3841 tttcttttgt ttatactaca ccttctcatc aatacccatt aggagggatt ttacctattc
     3901 aaagaagact agagcttatt aaatgtgcaa cagagaatga ttactatata attgaagatg
     3961 attatgatag tgaatttaga tatgagggtc aaccaattag ttctctttat gaacttagcc
     4021 caaataaagt aatttacata ggttctttta gtaaaatatt atctccagct attcgtgttg
     4081 gatttatgtt attaccagac aatattttag aaagctataa aaccttaaaa atgtactctg
     4141 atgttcatac agaaacaata ttgcaacatg tattagctgg atttattgga aatggaggtt
     4201 tagagaagta catatttaga atgaagaagt tgtacaatag aaaacgtaag tacctcataa
     4261 aagttttaaa cacggaattt aaaaatgaat ttaaaataaa aggtgaagca gcagggcttc
     4321 atatagttgc tcatttctat aatgttaaat ttacggaaag tttaattcat aaaatttttg
     4381 aagcaggtat aaaagtttat cctatacaac aatatgcaat tgagaatata ttagaatatg
     4441 actatgaaat tatactaggt tatgctcatc ttagttttga tgaaattgaa aaaggtgtaa
     4501 aaatactcag caaaataata catatgaatc aaggtacata gataaaacat gtagcaatct
     4561 acatataata gatgtaatta agtttaaact ttaaaagaaa tatatattaa tatttttgta
     4621 taaaaatacc tgatattttt ttatcgatac tttattgcta tacactaaat taagatagca
     4681 atttatatta agaaaaaact ttttgcatag gttataatac ttaaaaggag aggatagagt
     4741 tgaaaacaaa tacagatgtt attatggaga taattgatta tatagaagaa aatcttacag
     4801 aaaatctaaa tttagataaa atagcttata ttgctaatta ttcaaaatac catttacaca
     4861 gaatgtttac aaatgttgta ggatgtacta tccatcaata catacagagg aggagactta
     4921 ctgaagcagc tagacaactt atatttacag acaaatatat tatagacata gctattacag
     4981 taggatattc tacacatcaa tcttttacac tagcatttag aaatttatat aaagaatctc
     5041 cacaagtata tagaaataaa gatgaatttt atcctattca gcagagattt gttttaaatc
     5101 taaatcatca aattcaaagc gaaaaatgta tttcttatgc aaatatgaat tataaacaaa
     5161 gaagagatga aatcaattct tatctgaact ttaatattaa ttgtgaggtg aaagtagcat
     5221 gagtttagtg ccacatgtaa ttgaacaaac aggtcaaggg gaaaggtctt atgatatata
     5281 ttcaaaatta ttaaaagata gaattatatt tataggagaa gaaattaatg atgctattgc
     5341 aagtttagta gtagctcaat tactgttttt agaatcagaa gacccagatt cagatataat
     5401 tatatatata aatagccctg gtggctctgc tacatcagga tttgctatat atgatactat
     5461 gaattatgtc agatgtgatg tatcgactat ttgtattggt atggctgcaa gcatgagtgc
     5521 ttttttactt gcaggaggaa cacatggaaa aagatgtgct ttacccaatt cagagattat
     5581 gattcatcag ccaatgggag gagctaaagg tcaagcaaca gatgtaaaaa tagctgtaga
     5641 taatatattg aaaataaaag aacgattaga taaaattctt tctgaaaata caggtaaatc
     5701 aatagaagat attaggagag atacagatag agataatttt atgactgcac tagaggctaa
     5761 agagtacggg ctgattgatt atataatgaa taggaatgaa taaagtatca aagtatcata
     5821 ttaaaatgtc tgttttacaa gtatatatat aatattaaat aagagtgtct caaaatgaac
     5881 tttttaattt atgggacact cttttttgta tgaaattaaa tatggtttta tcatttcaac
     5941 aattattacc aaatttcaag tttaataaat tctactataa aaaatacata aaagataagg
     6001 ttctttagta actttctgtc gtcaaaagtt tttataaatt taataacaaa acaagagaaa
     6061 ataccatata ttacttatgt ctgcaatata tagaagacaa aggagttgta cattatgatt
     6121 acaataagtt tgtgcatgat tgttaagaat gaggaagaag ttataggcag atgcttagaa
     6181 tgtgtaaagg atatagtaga cgaaattata attgtagata ccggttctac agattccaca
     6241 aaagaaatag tctcaaaata tacagacatg gtgtacgatt tcgaatggat agatgatttc
     6301 tctgctgcaa gaaatttttc attttctaaa gcttctaaag actatactat gtggcttgat
     6361 gcagatgaca taattttaga agctgataga gaaaaattat taaagattaa agaaagcttg
     6421 gatacttcca ttgatattgt tatggcaaaa tacaatgtat cctttgatga aaatggtaac
     6481 ccaactctct cgtattatag agaacgattg tttaaaagaa gttctaatca taaatgggtt
     6541 gaccctattc atgaagtagt acctcttttt ggagaggtat tttattctga cattgcaata
     6601 tctcataaaa agcttcatag acaggaccct ctaagaaatc ttagaatttt tgaaaaaatg
     6661 atttcagaag gaaaaacatt ggagccaaga caccaattct attattctag agagttgtac
     6721 tataatgcta ggtataaaga agcaatagaa ggttttacta agtttttaga ctcatcaaga
     6781 ggatggattg aggattgtat cagtgcttgc agagatttag ccacatgtta ttacttaatt
     6841 aatgatgaaa aaagtgcctt atattcattg tttagaagtt tcgaatttga tgagccaaga
     6901 gcagaaattt gttgtgacat aggaaagcat atgtttgata gacaaaaata taaagaagcc
     6961 attttttggt ataaggtagc attaacaaga gataagaatg atactaatgg aggttttaag
     7021 tcaaatgatt gttatggata tataccatct atacaattaa gtgtctgtta tgacaggtta
     7081 ggagaatcag ataaagctat ttattatcat gaaaaaacaa aagaaatcaa acctaacgat
     7141 agtgctgttt tgcacaatga aaattatttt agtaaattta aaaatagttg ataggtcaca
     7201 atcatactaa atttgacata ttatatatag agcttaaaag ctcaaatata tcaggaggtg
     7261 cttttaataa tgagtagaaa taaatatttt ggaccatttg atgataatga ttacaacaat
     7321 ggctatgata aatatgatga ttgtaacaat ggtcgtgatg attataatag ctgtgattgc
     7381 catcattgct gtccaccatc atgtgtaggt ccaacaggcc caatgggtcc aagaggtaga
     7441 accggcccaa caggtccaac gggtccaaca ggtccaggag taggggcaac aggcccaaca
     7501 ggaccaaccg gtccgactgg tccaacagga aatacaggga atacaggagc aacaggatta
     7561 agaggtccaa caggagcaac aggggcaaca ggcccaacag gagcgacagg agctataggg
     7621 tttggagtaa caggtccaac aggcccaaca ggagcgacag gagcaacagg agcagatgga
     7681 gtaacaggtc caacaggtcc aacgggagca acaggagcag atggaataac aggtccaaca
     7741 ggagcaacag gggcaacagg atttggagta acaggtccaa caggcccaac aggagcaaca
     7801 ggagtaggag taacaggagc aacaggatta ataggtccaa caggagcgac aggaacacct
     7861 ggagcaacag gtccaacagg ggcaatagga gcaacaggaa taggaataac aggtccaaca
     7921 ggagcaacag gagcaacagg ggcagatgga gcaacaggag taacaggccc aacaggccca
     7981 acaggggcaa caggagcaga tggagtaaca ggcccaacag gagcaacagg agcaacagga
     8041 ataggaataa caggcccaac aggtccaaca ggagcaacag gaatagggat aacaggggca
     8101 acaggattaa taggtccaac aggggcaaca ggagcaacag gggcaacagg agcagacgga
     8161 gcaacaggag taacaggtcc aacaggagca acaggggcaa caggagcaaa tggattagta
     8221 ggcccaacag gagcaacagg agcagcagga acacctggag caacaggtcc aacaggagca
     8281 acaggcccaa caggagtagg aataacaggg gcaacaggag cgacaggtcc aacaggagca
     8341 gatggagcaa caggtccaac aggagcaaca ggaaatacag gagcagatgg agtagcaggt
     8401 ccaacaggag caacaggagc aacaggaaat acaggagcag atggagcaac aggtccaaca
     8461 ggagcaacag gaaatacagg agcagatgga gcaacaggtc caacaggagc aacaggagca
     8521 acaggagtgg caggagcaac aggagcaaca ggtccaacag gagcaacagg ggcaacagga
     8581 gcagatggag caacaggtcc aacaggagca acaggagcaa caggggcaga tggagcaaca
     8641 ggtccaacag gagcaacagg agttacagga gttacaggag caacaggccc aacaggagca
     8701 acaggagcaa caggagcaac aggtgctagt gcaataatac cttttgcatc aggtatacca
     8761 ctatcactta caactatagc tggaggatta gtaggtacac ctggatttgt tggatttggt
     8821 agttcagctc caggattaag tatagttggt ggagtaatag accttacaaa cgcagcagga
     8881 acattgacta actttgcatt ttcaatgcca agagatggaa caataacatc tatttcagca
     8941 tacttcagta caacagcagc actttcactt gttggttcaa caattacaat tacagcaaca
     9001 ctttaccaat ctactgcacc aaataactca tttacagctg taccaggagc gacagttaca
     9061 ctagctccac cacttacagg tatattatca gttggttcaa tttctagtgg aattgtaaca
     9121 ggattaaata tagcagcaac agcgcaaact cgattcttac tagtatttac tgcaacagct
     9181 tcaggtcttt cattagttaa tactgtagca ggatatgcaa gtgcaggaat tgcaataaat
     9241 tagattagat aagaaaaggc ttctaatatc gtattgatat taggagcttt tttaagaatg
     9301 attgatgaac atagttttaa tattaattag tgcgcatatt ataaaatact attatataat
     9361 ataaaataat aacattaaat tggaaatata gaattatcta ggggtgatac tatgaaaaat
     9421 aagattgaaa tatcaatgaa caactttgta aaaaattatc ataaatcaaa taatataact
     9481 acaaagtggc aagaactttt gtttgcttat gcatctgctg aagatgatat gtttcagaag
     9541 ctaaaaagtg ttgtaggaac atctcatgct atgccaaaag attttctgga aaatggaaaa
     9601 actataatta catattttat accttttgat gaaagtgtaa ataatagtaa tattggaggg
     9661 tttgaatgtt ctgaaatatg ggcaagagcg tacatagaaa ctaatatgtt gatttcaaat
     9721 ttaaatgatt tcttatgtga agaactagag aagatagggg ctaattcata tgctattcct
     9781 gggtcgtata attttgacag tgaaaaattg gtaagtgact ggtcacaaag acatgtagca
     9841 ttcatagcag gtcttggaac ttttggttta aataatatgc ttattacaga aaaagggtgt
     9901 tgtggaagaa taggaagcat aatcacaagt ttagaattag agccaactaa aagaccagaa
     9961 aatgaatact gcttgtataa acataataat agttgcaaaa aatgtgtcaa taaatgcgtg
    10021 aaagaagctt taaaagaaga tggatttaat aggaataagt gttatgaaat gtgtatgata
    10081 aatgaaaaaa aatacactta tataggtgga tgtgatattt gtggaaagtg tatagttgga
    10141 gttccttgtt cagtaataaa cccatcaaga taatggactt aagttgacta ataattttaa
    10201 agatagttta taatgaaaaa attttagtta tcaaaataaa tataatataa aaactaaata
    10261 aatgtgttta aaaaatggat aaataatttg tgttgattga caaccagtta tactgttgat
    10321 agacttatat gtatcgctgt gatatataag gagggttgag attttatgtt tgatttaatc
    10381 tttatttttg catagcaagg gctattgcaa ctatgaaatt taatatataa atagcccttt
    10441 gctattccta atgaaaaatt actatttaat aggagagcaa aatgagctat aaaaaagcaa
    10501 tacatatatt accagaagaa ttacttgaat taatacagga atatgtagat ggtgaatgta
    10561 tctatattcc tagaaaatct aataataaaa aagaatgggg aagtaataca cctactcgtg
    10621 aggagttgtc aattagaaat atgcagattt acgaagacta tcaagttgga tatgatttgc
    10681 aatatttatc ggaaaagtat tatttatctt tgaaaagtat ccaaagaatt gtattacaag
    10741 aaaaaagaaa agatatctaa ggatgagcca gtatagtttt ataattatac tggctcattt
    10801 tcttttgata aaagtgtttg aggtatagtt tcttttaagt ggatgatata atttaagtaa
    10861 tttcaataac ttatacagta gctaacaaag ctggttgtaa agtatctaat aagaagtagt
    10921 agctttttat atgtttgaaa ttaaaattta ttaaaattag gaggtcttgt tttgaaaaaa
    10981 atacttgatt atattccacc ttttcataac tttctattaa tttagtaatc ttatagaaag
    11041 gaaattgcat ttatgaaaaa aattacttgg gaggtggaat atatctccca gttacctgta
    11101 attaggtatt gtaaaaaatg tggaaaaaag actgaatata tttgttcagg tttatttagg
    11161 gttaatgctc aacataaata tttggatata tggttaatat acaagtgttc aaattgtgat
    11221 tcaacatgga actcaactat atattctcgt gttaatccaa aaagcttaaa tccagaaata
    11281 ctagaacaat tccatacaaa tgattataat cttgttaagc aatatgctat gaatatggaa
    11341 cttttacata gaaatggagc agaagttggt attccagagt ataaaatagt aggacaagaa
    11401 attaatatta atgaccttac acaagttcat attattagta aatatccatg tcagttaaag
    11461 gtatcaacat tattacgtga gaaactaggt ttatctagga aggtatttga cgaaatgttg
    11521 gattgtggga ttattagaag taactcagga gttgatttaa agaaaggtaa attaaattat
    11581 gaaattatgt tagatatagg caaggagaag caaaagtctt caaattgatt agaataacta
    11641 acttaatata ttttcaggaa catcatttaa aaaaatagtg actttaaaaa atttaaagcc
    11701 actatttttt aa
//
LOCUS       DAEMHZ010000127        11653 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000127 DAEMHZ010000000
VERSION     DAEMHZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.127"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..791
                     /locus_tag="KPK72_003053"
     CDS             15..791
                     /locus_tag="KPK72_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2490247.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(883..1635)
                     /gene="xth"
                     /locus_tag="KPK72_003054"
     CDS             complement(883..1635)
                     /gene="xth"
                     /locus_tag="KPK72_003054"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG2490248.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1796..2746)
                     /gene="galT"
                     /locus_tag="KPK72_003055"
     CDS             complement(1796..2746)
                     /gene="galT"
                     /locus_tag="KPK72_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG2490249.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKQLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3131..4327
                     /locus_tag="KPK72_003056"
     CDS             3131..4327
                     /locus_tag="KPK72_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2490250.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4652..5104)
                     /locus_tag="KPK72_003057"
     CDS             complement(4652..5104)
                     /locus_tag="KPK72_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2490251.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(5416..6861)
                     /locus_tag="KPK72_003058"
     CDS             complement(5416..6861)
                     /locus_tag="KPK72_003058"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG2490252.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIINDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(7005..7913)
                     /locus_tag="KPK72_003059"
     CDS             complement(7005..7913)
                     /locus_tag="KPK72_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2490253.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            8170..8994
                     /locus_tag="KPK72_003060"
     CDS             8170..8994
                     /locus_tag="KPK72_003060"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2490254.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            9034..9564
                     /gene="lepB"
                     /locus_tag="KPK72_003061"
     CDS             9034..9564
                     /gene="lepB"
                     /locus_tag="KPK72_003061"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2490255.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(9646..11427)
                     /locus_tag="KPK72_003062"
     CDS             complement(9646..11427)
                     /locus_tag="KPK72_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG2490256.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         3930 a         1739 c         1414 g         4570 t
ORIGIN      
        1 aggagataaa ctttatggaa tacataattt tcgatttgga atttaaccaa ggatttgata
       61 aaaaactcaa taaaactgta tccaatgaaa agtgtccttt tgaaattata caaataggag
      121 caattaaatt ggattctaaa ttcaatataa tagatacgtt taatagttat gtaaaaccta
      181 caatttataa agacatacat ccctttatta gtcgtatgac taatattaaa aattctgatt
      241 ttaatgattc gcctactttc cctgaggtct acaacaattt tataaaattt atatcatcac
      301 aagaccctat attatgtgtt tggggagctg gagatttaaa ggaattatac aggaatatca
      361 actatcacaa gctaccatca aattccttac ctaaatctta tattaatata caacaacatg
      421 catctaaata tttcaataat ccagcaggaa aaagcattgg cctacaaaat gctatttcta
      481 tattagaact tgatgaaaaa atgtcttatc acaatgcttt aaatgatgca tattatactg
      541 ctaaagtttt tattaagata tacaatccaa gtatagtccc agatatatat ctttatacga
      601 gtataaaacc taaaactata agatattcta ataaaaaaag agttgattat gataagttat
      661 ttgatgaatt taggaaaatt ttaaatcgtg aattaactaa agacgagaaa aaaatcatta
      721 acttagctta taatatgggg aaaacaaatc aatttacatt agaaaatgtt aagcaaagaa
      781 aaaacaaata gagtttattt agataaaaaa taggcatgta gattaaaaaa ctagttaact
      841 ttaaatttat atctacagcc caatttttat cataattaat ttttatttta tctctaattc
      901 aactggacaa tggtctgaac ctaatatttc agtgtgtata tccgcactta ctaatctatc
      961 ttctaatttt ttggatgcac aaaaataatc tattctccac cctgcattat tttttcttgc
     1021 attaaatcta tatgaccacc aactataaac accttcttta tcaggattaa aatatctata
     1081 tgtgtctata aacccactgt ctaataattt agaaaacttc tctctttctt ctttagtaaa
     1141 acctgcattt ttcatattat ttttaggatt ttttaaatct atttctttat gtgcaacgtt
     1201 catatctcca cacacaatta caggcttatg attatctagt ttggttaaat aatctataaa
     1261 gtcgtcttcc catctagttc tatattctag tctctttaat tcagattgag aatttggcgt
     1321 atatactgta acaaagtaaa aatcttcaaa ctctagagtt atcactctac cctctttgtc
     1381 atgttcttct atatttattc catacattac ttttaaaggc tctttttttg taaatatagc
     1441 agtacctgaa taaccctttc tctcagcata attccaatat tgaaaatatc ctggtaaatc
     1501 taactctatt tgcccttctt gcaatttagt ttcttgtaaa caaaatatat ctgcatctac
     1561 ttctttgaaa aagtctaaaa atcctttccc aacacaagct cttattccat taacattcca
     1621 tgatataaat ttcatactta tactcctcat ctaaacattg acacactccc catagctaaa
     1681 gatgggggct tttcaacaat tttattgtaa attcttaatt tacttttatt ttaacaatgc
     1741 tatactataa tttcatctaa aattactatt tacttaatac ctaaaaatat atgttttaat
     1801 caatttttaa cttttttgtt aaatcttcag ggctcattga agaaacatac atatttgttc
     1861 caagttcaaa tcctccaaaa ttatattttc ttgggattat gtgcatatga acattaaaat
     1921 acgattttgt ttcaatcttt gttggatgag tgtgaataca taagttaaat ggcatatatc
     1981 cattaatatt gtataactgc ttaaacaact tttcaaatat tgtagaaagc tcctgtatat
     2041 tattatcatt taactcacca aactctattt tatctttaaa tattattctt acttctccac
     2101 tatacttggt tgcatatggt ataagtacca aaaaagcttc tccattataa acaactcttc
     2161 tatctaaact tatttcattt tctattaaat catcatacaa agagctttta ttgctgtcat
     2221 aatactcctt tgaaaccaac aattcattcg taatttcagg tggaataaaa gacatagaca
     2281 atatttgagc atgtggatgc atcaaagatg ctcctgcctt tctcaagaag tttttgaaga
     2341 tactaacata ctctacttta tcatctttcg acaaatctct aaatctattt ctatacataa
     2401 aaaaaacatc ttcaaattct ttttgactca tgttataaaa actacgatta tgtttatcgc
     2461 tctctatcat tacttcatga acaccatata catctcttgc catatcatca attataggaa
     2521 atttattgta gacagatttt acaagccatc cttcttcact atctatttca aataatgcat
     2581 cagttgcatg agcttcattt cctttgcaaa atgggcattc ttcatcatat tcattagttt
     2641 cttcatcttc atttgggata tctgctgtat ctaaaggtct ttttaaccta tctgtagcaa
     2701 atataacaac atcattggta attggatcta ttcttaattc tttcataatt aacacctctt
     2761 agttaatagc ctttaatata attatatgat atttatcgta tctgttaaat aaatttataa
     2821 tattttttat aattttgcat atttattatt atatattttg tctatagctg aaagattata
     2881 actaaaaaat cactatcagt atatcagaat attatcttac tataaattaa tttatgaata
     2941 cataatttag tggcataaac atgggtattt tactataatt tgttaaaagt ttattttaaa
     3001 gctatattaa ttttttttga tgatttttac ccataagtat gttattatta tcttgtaaag
     3061 atgtcttgta ttcaaacata caatttacat aatacaatat acgtatttaa attaaatagg
     3121 gggtattaaa atgaattatt caaacagagt tagtgcaatg caagcctctc caatcagaaa
     3181 acttgttcca tttgcacaag cagcaaaaga taaaggaatc aaagtatatc acttaaacat
     3241 aggacaacca gacataaaaa ctcctaaagg attttttgat gctgttaaaa actttgatag
     3301 cgaagtctta gaatatgcta catcagaagg tattccagaa ttattagaag cacttcaaaa
     3361 ttactataaa acttacgata tgaattttga aaaagatgaa ttattagtaa ctaatggtgg
     3421 tagtgaagct ttacttttca ctatgatggc agtttgtgac ccaggagata atctattagt
     3481 tccagaacca ttctatacaa actacaatgg atttggtcaa tctgtaaacg tagaagttaa
     3541 tgctgttaca acaaaggcag aaaacggttt ccaccttcca tctaaagaag aaatattgtc
     3601 taaagtagat gataaaacta aggctataat tctttctaac ccaggaaatc caactggtgc
     3661 aatttataca aaagaagaac ttaatatcct agctgaaata gctaaagaaa aagatttatg
     3721 gataatagca gacgaagttt atagagaatt tgtttatgat gggcttgagt atacaagctg
     3781 tggtaactta gagggtgtac aggatagagt tataataata gacagtgttt ctaagagata
     3841 cagtgcttgt ggtgctagaa ttggttctat cgcttgcaaa aataaaggtc ttatcgctca
     3901 aatacttaaa ttatgtcaag gaagactttg tgttcctaca ttggagcaaa taggagctgt
     3961 tgagttatac aaaactcctg tttcttactt caaagaagtt aatgaagagt acaaaaaaag
     4021 aagagatgta ttatacaatg aattaatgaa ggttgaaggt gttatatgta aaaaaccaac
     4081 tggtgctttc tatatagttg ctaaattacc agttgaaaat gcagaagatt tcacaatttg
     4141 gatgttaaaa gaatttaaca aagataatga aacagttatg gtttgccctg ctgaaggatt
     4201 ctatgctact ccaggtttag gacgtgatga aataagatta gcttacatct taaatgaaaa
     4261 agaccttcat agagctgcta cattattaaa agaaggttta gagcaatatg tagctttaca
     4321 aaaataattt tttaaactta atttatataa atttaatgat ataaaatgtc cccttttcag
     4381 atattatttg aaaaggggac atttcttaat taatatatta attaagactt agacctgact
     4441 atttttcatt ttctttatat agtgttggcc agtaatcttt attcacttta attaaagcat
     4501 ctaaaacttg tcttgcctta gttagagaaa ttataaaaaa gtaatttaag atgtactttt
     4561 tagattgtat ttagacttat aattatatag ttaaatgaat aacaaagaat taatcttagt
     4621 accattaaat acaaaaattt ttatattttt tctatctaaa ttcttctttg tttaatatat
     4681 attgaagagt atttattgct tctaaatatt tttttctgtc ttcttcagtt aatttagaaa
     4741 gtttttctcc taaaccatca cttaatttat ccataaaatc agttgctatc ttatctccct
     4801 cttcagtaat gtcaattaga tattttcttc tatcaactaa atctcttttc cttgttacat
     4861 acttcttatc agttaaatca tcaaccatac tagttaaact gcctttttct atatttaact
     4921 tagagcataa atcagtcata cttattactc cataattttt tatgaaaact aatgctctta
     4981 attgtgtttt atttacatta taatctggtg caaatttttt taggtcttct aagtacaggc
     5041 tagaataaat cttaggaaat gtttttacta aaaaacttat ggtatctttt actatatagt
     5101 tcatataatc acctcggttg ttttaaaatt tattaaatta tctaaataac tttatttcag
     5161 tttacattaa aaatattgca agtacaatag tttttgctga atgtttaaat ttaattagta
     5221 atcttatcat ttttttattt ttatttatat tattcctaat taaatattat ttatatagct
     5281 attttattaa attcatggta tagtaagtaa agaagaactc ttcacagcgg tggagagaag
     5341 acgctagccc attaaaatgg ctatcttatt tataaataag atagccgttt ttaattatat
     5401 tttatctgta caattctata ctcttaggtc tacatctact tttcctaagt cttccttata
     5461 atcttttata gcaggttcta caacttcatt tacaaattct acaacttgtt ctggagctct
     5521 acctataaag ttttttgggt ccattataga tagtatttct tccttgctca ttttaaatga
     5581 atcatcattt attataagtt caattaagtt attatccttg ccttcatgct ttactctata
     5641 ggcagctttc atagaatatt ctcttattat ttcatgtaat tcctgtctat ctccacctct
     5701 ttttacagct tccataagta ttgtttctgt agccataaaa ggaagttctt catttacacg
     5761 tctatttatc atattttcat atactactaa accatcagtt acatttatcc ctatttcaag
     5821 tatagcatct gctgccataa atgcttgagg tattgccaat ctcttattgg ctgagtcatc
     5881 taatgttctc tctaaccatt gagtggcttg taccattgca gggcttatag attcagagat
     5941 tatatactta gataaagatg ctattctctc acttctcatt ggattcctct tataagccat
     6001 tgctgatgaa cctatttgat ttttttcaaa tggctcttct aattctttta aactttgtaa
     6061 aagtcttata tcattagtca tcttatgcat actttgagct atccctgata gtatgcttat
     6121 tacttgataa tctaactttc tagtatatgt ctgtccactt actgcatagg aagaatggta
     6181 tcccatcttt ttacaaattt tcttatctaa ctcttttact ttttcgtggt ctccttcaaa
     6241 taagcttaaa aaacttgctt gagtaccagt agtaccttta actcctctta acttcatgtt
     6301 gtctattcta tagtttaaat cttccaaatc aagaattaaa tcttgagccc ataaacaagc
     6361 tctttttcct actgttgtaa gttgagcagc ttggaagtgt gtaaatccaa gagctggcat
     6421 atctttgtac ttaagtgcaa attcttttaa attgtttatt aaatttacaa gctttactct
     6481 tagaagtttt aaagcttcat tcatttgtat aacatctgta ttatctccaa catatgcaga
     6541 tgttgctcct aaatgtatta ttcctttggc tttttcacat tgtaaaccat aagcttttac
     6601 gtgagacatt acatcatgtc ttacttcttt ttctattttt ctagcttcat caaaatttat
     6661 attatctata ttagctctta gctcatctaa ttgttcttca gttatattga ttcctaactc
     6721 atgttcacct tctgcaagtg caacccataa ccttctccat gtagagaatt taaattgtgg
     6781 agaaaatata taactcatgt ccttgctaca atatctgtct gtaagtgggt ttgagtaagt
     6841 agtatattta ttatctatca ttatattagc accttcctta aatcttttgt cgtgtgatta
     6901 ttgtgtacac attagtacat aaataattat atataatttt acgaactata acaagacttt
     6961 tttatttttt gttcacattt atatatagta ttttttattt attactattt tttatttata
     7021 tccaaattat ctttttcaat taagtaatcc actatttcac tatcaacaaa atttaataca
     7081 tacttatatc taattgcata gtatttatca ctccatccaa gctgattttt tacatcactt
     7141 tcatctgccc caagccttaa acttattgct gcaaatgaat gtctaaaatc tcttgctgaa
     7201 tattgagaaa gtccagctaa atctaatgct tttttgacta taagtctgac atttctatct
     7261 gttatagaat tactcttttg agttgtaaaa ataaaatcat cactaggtat tattacctct
     7321 ggaagcccag aataattcct gtaatcttct aaaagtttaa aaaagtatgg atgtaattta
     7381 actactcttt cttttcttcc cttacctaac cttacatatg agttatcgtt ttcatctacc
     7441 ataaggtctt tccactttaa gctaactaat tcatttaata aacacccagt agtaactaga
     7501 cagactatta ttactctatc tcttatattt aattttggta ggatacctat aagtttattt
     7561 atttcttgta tacttagaac atcatctttt gtttttattc ttgttactgt gggtttttct
     7621 acatatctaa atggatttat atctatgtat ccttcttttt ttaagaagtt ataaaaacta
     7681 tgtaaataac tatatatctt ttcacaagtt gatttcgcat atttagtctg tatatagtct
     7741 acaaaatttt tacaatcttc tttcgtagca taaatcaatt ctttcccttt taaaaattct
     7801 ttaaacagta taacctttga taaataatcg tgtttcgtgt ttggatttaa ttttgtagaa
     7861 aaaacattaa aagtttcata ctcataatca tctagtattt ttgtactata cataataact
     7921 cctttccttt tcataaaata aaatagctat acttttacta taccatatat ttccccctca
     7981 aatatacccc ttattcaata gatgactttt aagtttattt tgttatatta tgaaggtttt
     8041 atgaaaatat caatcttcca ttgtgcacta cacagtacta tggtattata agttagtaaa
     8101 aggtggcatt tttttattaa tctatgaata aaatatgcta ctataaattt gtattggagg
     8161 tattttgatt tggaaacatt tagtttgctt gaaattttta aggcagtcat tttgggcata
     8221 gttcagggaa taacagaatg gctaccagta agtagtacag ggcatatgat attaattaat
     8281 gagtttataa aattgaattt ttcaaataca tttataaata catttctagt tgtaattcaa
     8341 tttggttcaa tattagctgt acttgttata ttttttagaa aattaaatcc ttttgatagt
     8401 gcaaaaaata ttaagcagaa aaaagagaca gtccgtttat ggcttaaggt tataattgca
     8461 gttataccat ctggagttat tggaatactg tttgaagatg atatagatag agtgtttttt
     8521 aattcaacag tagttgctat tgctcttatt gtatatggta taataatgat agggcttgaa
     8581 aagagaaata aaagacctaa gtataaagat ttttctcaag ttacatataa attagctctt
     8641 tgtataggat tattccagtg tctagctctt ataccaggta catctaggtc tggttctact
     8701 ataataggtg cagtattact tggtacttct agatatgtag cagcagagtt ttcattcttc
     8761 ttagctatcc ctacaatgct tggagctagt gcattaaaac ttctaaaagc tggatttgga
     8821 tttactggat ttgaatggct tattttaggt gttggttctg tagtagcatt tgttgtttca
     8881 attgtagtta ttaaattttt tatggactat atcaaaaaac atgacttcaa agtatttggt
     8941 tattatagaa tagtcttggg tatagtggtt cttgcttatt tctttctatt ataatgtatt
     9001 ctgatttaat aacaaatgat aaggagaaaa gttatgagtg ttaaaaaaga aatatttgat
     9061 tggattaagt caatagctat ggctattgta cttgcatttg taattctaca atttataaga
     9121 ccttctattg taagtggaga atcaatgtat cctactttag atgataaaga ttatctgatt
     9181 ttaaatagga tatcatacaa ggttggtaaa cctgaaaaag gcgatattgt agtttttaaa
     9241 accaatttag ttgatggaga aacaggaaag aaaaaagact taataaaaag agttatagct
     9301 actgaaggtg acagaataaa aatatcaaat tctaaagtgt atgtaaatgg aaaattatta
     9361 aatgaaccat atatacacaa taactatact tctggagata tagatactgt tgttccaaaa
     9421 ggtaaactat ttgcaatggg agataataga gaaaatagta atgatagtag attccctgat
     9481 gtaggtatgg ttgatgaaga tgaagttctt ggtaaggtta tggtgagact attacctctt
     9541 gataatattg ggaaagtaga ctaaaaaata agagactgaa ttggtaaagt ttattttaca
     9601 aatctcagtc tctttttatt ttcttaaatt acaaatattt ttttactaaa tatcttttga
     9661 ttctgtaata agtcttatta tatcacttgc ttcttcatta aaagttgtta ctatacttag
     9721 gtagtctgtt gctctactca tagctaaata aaccttattt aaatctccta caaagtcatt
     9781 tacttgataa tcttgttcaa tatcatttat agtttgattg tataacatct ctaattcaca
     9841 tactattacc gctttatact ctaaattctt tattgcatat atatttgcta ttgtagttcc
     9901 taccttttta cttatatttg tcaaattatc ttctgcacat atataaggaa tttttgcctc
     9961 ttccaatgct ttttttaaca tatattgaaa gtaaatggtt tttccacttt ttaatttctt
    10021 cttattgtaa ggataaacga tagcaacttc agaataatca agaccttttt tacttattaa
    10081 atactcaatt tcccagatta cagaacttat ttggtcatca aggtcactta cttttattat
    10141 atcaacagac ttatttccac ctctaagtgc tttagtataa aagaaagtac taaattctgt
    10201 atttggtctt aattcgttga tatatgagtt agaattattt gaaaatttat ttgtaaaatc
    10261 tacaatctct ttagcttgtc tataattttt acttaagata atcttatcat caaattctat
    10321 accattatat aattttttaa atatattcaa tctattagag atattcaagg agttacaaga
    10381 acacacatta aatatgaatt ttgttttata taaaaactcc cttataaaat ctatttcata
    10441 ctctaaaaag ctttctgctt catcaataaa aatagccttg aacatatttt tattttttat
    10501 aatgttctgt gcttgtttta caagattatt aaaggtcttt tcgtaatcat tttttaacat
    10561 gttataatct acaattaaat tgaatttttt agctagttta aatataaaag aactaaatgt
    10621 gtgaacttct aaattgttat tatctttgta taataactct atcctctctc gaagctcatt
    10681 gcacaattgt tttgtatgtg taaatatgac taatttatga tgtggatata cttttgctaa
    10741 ttttatagcc ctagaaagca taattgctgt tttgcctata ccagaaccac cttcaataag
    10801 tgtattccca tatttaatag aaacaacatc tttaatttga acgtcttcca tcatagttgc
    10861 tgtatactta tagtcatcat tgtagaatga aattttttta aatttttcat ttagatgaag
    10921 tttatcattt attatatagt attctgaaca tatgtctagt aaaaataaat ttaagtttat
    10981 ttcatcattc tcatcattaa gatattcctc cagcgaaccc ttattcatta tatcttgaag
    11041 cttattttta tctattatat tattattaac aaattcttca aattcatatg tttcctcaac
    11101 ttcaacgtat ggcataatgt aaacgtagtt ataacttata tttctattct tttgtcccat
    11161 ttttagtttc aaaagttcat attcctcatt cataacttca agtaactctt cttctaaaat
    11221 aaaaaatata tcttctgttg tatccataaa ttttataaat aaaattcttc catctttaac
    11281 atacaacaaa tcggtattta ttcctttaaa aggagtaact tttggcacta ctttaacgtc
    11341 ctcattgata ttttcgtaaa atattttctc gaactccttc aattttaggg cgtctaactg
    11401 agaatttata tttatgtatt ggctcactta cattacctcc ttctgttata cttaattata
    11461 ccattttgaa ctatattttt atatatactt tttataggca aatttagtaa ataaattcaa
    11521 aataaataca aataaataca aaactgctaa aataattttg caataaatat agatatttat
    11581 tgttatagaa tactttctgt aaataataaa acgataaatt tcataattaa tttttaaaat
    11641 aataagtaaa gtt
//
LOCUS       DAEMHZ010000128        11551 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000128 DAEMHZ010000000
VERSION     DAEMHZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.128"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            148..1461
                     /locus_tag="KPK72_003063"
     CDS             148..1461
                     /locus_tag="KPK72_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2490257.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1633..2808
                     /locus_tag="KPK72_003064"
     CDS             1633..2808
                     /locus_tag="KPK72_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG2490258.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2911..3930
                     /gene="hrcA"
                     /locus_tag="KPK72_003065"
     CDS             2911..3930
                     /gene="hrcA"
                     /locus_tag="KPK72_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG2490259.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3960..4580
                     /gene="grpE"
                     /locus_tag="KPK72_003066"
     CDS             3960..4580
                     /gene="grpE"
                     /locus_tag="KPK72_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG2490260.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4672..6519
                     /gene="dnaK"
                     /locus_tag="KPK72_003067"
     CDS             4672..6519
                     /gene="dnaK"
                     /locus_tag="KPK72_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG2490261.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6730..7884
                     /gene="dnaJ"
                     /locus_tag="KPK72_003068"
     CDS             6730..7884
                     /gene="dnaJ"
                     /locus_tag="KPK72_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG2490262.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8850..9785
                     /locus_tag="KPK72_003069"
     CDS             8850..9785
                     /locus_tag="KPK72_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2490263.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9821..11194
                     /locus_tag="KPK72_003070"
     CDS             9821..11194
                     /locus_tag="KPK72_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2490264.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFINIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11382..>11551)
                     /locus_tag="KPK72_003071"
     CDS             complement(11382..>11551)
                     /locus_tag="KPK72_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490265.1"
                     /translation="DLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYL
                     SVKSYFLYKKI"
BASE COUNT         4546 a         1258 c         2135 g         3612 t
ORIGIN      
        1 ttagaagtta cgattgtatg atttttaaat gcataggcat ttactctagg actatagact
       61 aatataaaaa aaaactttgg gaatactaaa accataaatt tataaaatta atattttata
      121 tactgtaaaa cagtgaggag ggacagtatg catactcaac tacaaaagac tattggtctt
      181 tcagcagctc tttctactgt tgtaggtatg gttataggtt cgggagtttt ttttaagcca
      241 caagcaatat acactacaac taatggagct ccaggtctag gtattatagc gtggctttta
      301 ggtggtttta taacaataac tgctgggctt acagctacag aaatttcagc tgctatacca
      361 aaaactggtg gtatgatgat ttatatcgaa gaaatatatg gagaaaagtt aggtttttta
      421 actggatgga tgcaaactgt attatttttc ccaggtacat cagcagcatt aggagtaata
      481 tttgctcaac aagcatctga acttttagga atgagcccaa ataatatggc aaatgtactt
      541 cctatagcaa ttggagttat actattttta gcattattaa atataatagg ttcatcctta
      601 ggaggaaaag ttcagacagt agccactatt ggaaagatga tacctttaat tttaataata
      661 gtctttggat ttattaaagg tcaaagtagt gaagtattaa atccatttgt aggagatggt
      721 gtaaatgcat caaatgcact tggacaagta ttaatagcta ctttgtttgc atatgatggt
      781 tggataaatg ttggagctat atcaggagaa atgaagtctc cagagaaaga tttaccaaga
      841 gcaatagttg gtggtttatc attagttatg gcagtttata ttataataaa tatagcttat
      901 ctttgggtag taccagcaag tgaattagca acagttactt ccccagctac attagtagca
      961 acaagattgt ttggaaatat aggtggaaaa gtaataactg tgggtatatt aatatcagta
     1021 tttgggacat tgaatggata tttattaaca ggttcaagga taccttatac attagctgaa
     1081 atgggaactt taccagcatc taaaactctt ttaaaagtaa attctggagg ttctcctgta
     1141 aattcaatac tacttataac tgtgttagcc tgtgtatatg cattatctgg tcaatttaat
     1201 cttttgacag acttaacaat attttcaata tgggtatttt atgtattaac ttttataggt
     1261 gttatgaggc ttagaagaca aaagccagat ttacacagac catataaagt ccctttatat
     1321 ccaataatac caattatagc tatattaggt ggactgtttg taataataaa tcaaatattg
     1381 acttcaacag tcatatcttt aggtggaata tttataacac tattaggttt accagtttat
     1441 tattatatga aaaaaagata actacttaat ttaagttaga taaatttggg taaaggtgaa
     1501 aattaacttt tatccaaatt ttttatactg ttatacaata taagtattat atattacttg
     1561 atgtgctatt ttgtttgatt tataatttag taattgatgt tttaagataa tttaagaaat
     1621 gaggagtgaa agatgctagg actttatgta catattccat tttgtgtgaa aaaatgtaag
     1681 tattgtgatt ttaattctta taaaatggac atagattcaa aaaaaagata tatagaagac
     1741 ttaaaaatag aaatggaatt atatagtaat aaattatata aagataataa atataaaaat
     1801 aaagaatgct gtagtctaaa taaaaatgat aaaataacga gtatatttgt agggggaggt
     1861 acacctagta tactaacctc tgatgaaata agagaagtat ttataagtat aaaagaaatg
     1921 tttgatatag atgagaatgc agaaataact atagagtgta atcctgggac attaacatta
     1981 gaaaaattaa aaactatgaa agagattggc ataaatagac ttagtatagg gctacaagct
     2041 attcaagaaa agcatttgaa ctttatagga agaatacata cttatgaaga atttgagaaa
     2101 aactataaag atgcattaag tgtaggtttt aaaaatatta atatagattt aatgtacagt
     2161 cttccaaatc aaactctatg tgattggaag gaaactcttg aaaaagttgt acacttaaat
     2221 ccaacacata tatcagctta ttccctgata ctagaagaag gaactgaact atacaatatg
     2281 tatgaaagta ataaatttga acttatagat gaaaatgtag atatagaaat gtatgagtat
     2341 acaataaact acttaaagtc aaaaggatat aatcaatatg aaatatcaaa ctactcaaaa
     2401 gaaggctata attgtgagca taatatttta tattgggaat gtgaacatta tatagggatt
     2461 ggagcaggag cttctggcta tattaatgag aacagataca ataatgtaga atctttagaa
     2521 gattatcatt taagtttggt aaaaagagaa aaaccaatac aagaaaatga aattctttct
     2581 gagaaagata tgatagaaga aaagatattt atggggctta gaatgaataa aggcataaaa
     2641 tttgaagatt ttaaaaaaaa atttggaata gattttagag agaagtataa taagcaaata
     2701 gaaatgctat tagctagaaa gcttataaat cagagctttg aaggtataca attaactcaa
     2761 aaaggaagag aaatatctaa tagtgtattt atagaattta tggaataatt tttaaggtgt
     2821 tgacaaaata aatttataat ggtatataat atatataatc tttttagcac tcgaacttag
     2881 tgagtgctaa cagctaggag gtataataat atggaattaa atgaaagaaa attaaatatc
     2941 ctcaaagcta tagttaaaga ttatatagaa acagctgaag ctataggttc tagaacaata
     3001 tctaaaagac atgatttagg tgttagtgct gctaccataa gaaatgaaat ggctgacctt
     3061 gaagaattag ggtatttaat tcagcctcat acttctgcgg gtagagttcc atctgaaaaa
     3121 ggctataagt tatatgtaaa ctctcttatg agtaaaagcg aattggatga taatgataag
     3181 attcttattg aacaatgtat gaatcacaat ataaatcata taaaagaatt gattcatgaa
     3241 acttctaagc tattatcgca actaacaaac tatacaactg tagcagttac taagagttta
     3301 ataaatcaaa gtgttataaa gcacatacag ttggttgcta tgaatgacaa taatatttta
     3361 cttatagttg taacagataa aggggactta aaaaaagcta accttacaac taatgtatat
     3421 ttagatcaat ctaagttaaa tttgatttct gataatctta caaggaagct cttaggtaaa
     3481 agtataacag atttggatga taatctaatt gcttttatta aatacgaaat aagtgagtat
     3541 tctgggctta tagatgagct tttaaatgct ttgaactcaa atatgaaaga agaagatttt
     3601 tctctatcat taaatggagc tactaacata tttagttatc cagaatttaa tgatgtatta
     3661 aaagcaaagt catttttaaa tatgttggaa aaaaaggaaa caatagctga cataattaaa
     3721 tcaaagggaa tacaaaaaga caatctaaat ataataattg gtagtgataa tgattgtgaa
     3781 ctggcacagg attgtagtat agttacagca acttataatg tagatagaga cttggttggg
     3841 cgaataagct ttataggccc taccagaatg gattatgcta gaatttactc tataataaat
     3901 tatatgagtt tattaattaa tagaaagtaa agaaatttta aattgcgagg tgtaaagtct
     3961 tggataaaaa aaatgagcaa caggaagtaa gagaagaaaa tgatacttct attaaccaag
     4021 aatcggaaac tcaagtagag ttagaagagg aagttgttaa tgaagaatgt gaaacatcaa
     4081 gtgaaaaaac tgatgaaaaa gaagttgatg atgaaaatgt aacagatata aattctaagt
     4141 tagcagaaaa aaaattgcag gatgagttgg atgaattaaa tgataaatat caaagacttc
     4201 aagccgaata tgcaaactac agaagaagaa ctcaacaaga gaaagagaca ataggcgtat
     4261 ttgcaaatga aaaaataata actgaattga taccagttat agatagtatg gaaagagcct
     4321 tagatgcatg tgaagataag gaagatacta tgtataaggg tataagcctt gtacataaac
     4381 aattaataga cactttagta aaatttggag tagaagaaat agaagcagaa tctaaggaat
     4441 ttgaccctaa tctacatcta gcagttatgc aagaaagtgt agatggagtt gaagcaaatc
     4501 aaatagtaat ggtacttcaa aaaggatata agctaggaac taaagttgta agaccttcaa
     4561 tggttaaagt ttcttgctaa gtataaatta aaggttaaat tatttaacct aatataaaat
     4621 attaaaataa gtaaaactta attatataat tactcgagga ggattttagt catgggaaaa
     4681 ataataggaa tagatttagg aacaacaaat tcatgtgttg cagtgttaga aggtggagaa
     4741 gcacaaataa tagcaaatag tgaaggtatg agaactactc catcagtagt agcatttaca
     4801 aaagatggtg aaagaatagt tggagaacct gcaaaaagac aagcagttac aaatgcagat
     4861 aaaacaataa cttctataaa aactcatatg ggaactgatt ataaagtaaa tatagatgga
     4921 aaatcatata ctccacaaga aatatcagca ataatattac aaaaattaaa atcagatgca
     4981 gaaagttact taggacaaac agtaacagaa gcagttataa cagtaccagc ttactttaca
     5041 gatgctcaaa gacaagcaac taaagatgct ggtagaatag cagggcttga tgttaagaga
     5101 ataataaatg agccaacagc agcagctctt gcttatggta tggataaatt agaccaagaa
     5161 aagaaaatat tagtatttga cttaggtgga ggaacttttg acgtatctat acttgaaata
     5221 ggagatggaa cttttgaagt tttagccaca gctggtaaca atagattagg tggagatgac
     5281 tttgaccaaa tagtaataga ttatttagca gaggaattta aaaaagctga aggtgtagat
     5341 ttaagaaatg ataaaatggc tcttcaaaga ttaaaagaag cagcagaaaa ggctaagaaa
     5401 gaattatcat caacaatgag ttcaaacata aacttacctt tcataactgc tacagcagaa
     5461 ggaccaaaac acttaaatat agatttatca agagctaaat ttgaagaatt aactagaggt
     5521 ttagttgaaa aaactatgga accaactaag acagctttac aagatgcagg actttctaca
     5581 ggtgatatag atgatgtatt attagtaggt ggttctacaa gaataccagc agtacaagaa
     5641 gctgttaaga agtttatagg aaaagaacct cataaaggta taaacccaga tgagtgtgtt
     5701 gcagcaggtg cttctataca agctggagtt ttagcagggg atgttaaaga cttattatta
     5761 ctagatgtta ctccattatc tcttggtata gaaactatgg gtaatgtaat gactaagata
     5821 atagaaagaa atactacaat accaactaag aagtctcaaa tattctcaac tgctgctgat
     5881 aaccaaacag ctgttgatat acatgttctt caaggtgaaa gaagtatggc ttatgacaat
     5941 acaactttag gaagattcca attaacagat ataccaccag cacaaagagg aatacctcaa
     6001 atagaagtta cttttgatat agatgcaaat ggtatagtta atgtttcagc taaagattta
     6061 ggtacaggaa aagagcaaaa aataactata acatcaaaca ctaatttatc agaagcagaa
     6121 atagagcaaa agataaaaga agcagaaatg aatgcagaag ctgacaaaca aaagaaagaa
     6181 aaaatagaag cattcaatca agctgaatct actatatatc aaactgaaaa aactttaaat
     6241 gaacttggag ataaaataag ttcaggtgaa aaagaggata tagaaaaagc tatagcagac
     6301 ttaaaagctg taaaagataa tcaagatgct acagcagaag aacttaagaa agctacagat
     6361 gaagtaatga ctaagttcca aaaagtatct caagaaatgt accaaaaagc agctcaagaa
     6421 caacaagcgg cacaaggtgc agaacaagca caagacaatg gacctaaaga tgacaatgta
     6481 gtagatgctg actttaaaga agtagatgaa gataaataag ctatataata tgtaacacta
     6541 aatgataatt ggaacatata tgatttgtga aaatcatata gatgttaaag atatatggac
     6601 aatatacatc ataaagaata taatatatca tagattatat aacttaaagt agaatataag
     6661 aattaatgat agcttgtagt ttattattct acaagctatc gttttgtaaa atgaaggtgg
     6721 tgtcaatttt tgagtacaaa aagggattat tatgaagtct taggtatcag taaaggtgca
     6781 gaagctcaag agataaaaaa agcttataga aaattagcaa tgaagtacca tccagataga
     6841 aatcctggag ataaagaggc ggaagaaaaa tttaaagaga taaatgaagc ttatgaagtt
     6901 ctatctgatg atacaaagag aaaaacttat gaccagtttg gacatgatgg gctaaatggt
     6961 caaggcggtt ttggtggtca aggaggattt ggcggtcaag gctttggtgg ttttgaagat
     7021 atgttcggag atatatttgg tgacatgttt ggaggaagct tcggaggtgg aagagcaaga
     7081 agaagagggc ctcaacgtgg tgcagatata agacagagtg ttactatatc ttttgaagaa
     7141 gctgcatttg gtaaaaagat gtctataaaa gtaaatagaa gtgaagagtg tgaagaatgt
     7201 aatggaactg gagcaaaacc aggaacatca aagaaaactt gttcaacttg taatggaact
     7261 ggtcaagtta gaactgttca aagaactcca tttggtaata tagcaagttc aagaccatgt
     7321 agtgcttgta atggaactgg agaagtgatt gaatctccat gtagtaaatg tcatggaact
     7381 ggaaatacaa gaaaagttaa gactatagaa gtagatatac ctgctggtat agatgatggt
     7441 caaatgataa aactttctgg tcaaggtgaa gtaggagaaa aaggtgctcc aagaggagac
     7501 ctatatattg tagttaatgt taagtcacat ccactattta ctagagatgg aaatgatatt
     7561 tattttgaaa tgccaattac atttgttcaa gcaactttag gtgatgagat agaagtacct
     7621 actttggatg gaaaagttaa atatagtgtg ccagagggga cacaaactgg aactgtattt
     7681 agacttaaag agaaaggaat ccctagaatt agaggtaatt caagaggaga ccaatatgtt
     7741 aaggttgtag ttgaaattcc taaaaaatta aacgataagc aaaaagaact attgagagaa
     7801 tttgcgaagg agtgtgggtc gaacgtccac gaaaagaaga aaaccttcgg acaaaagata
     7861 gaagatatgt ttaagaaaaa gtagttaaag tagccaatat ataatcatat ttttaaaatc
     7921 ttagttattt ataagattgt caaaggagat attatgattt ttatattggc tatttttgta
     7981 taaaaaataa atatgcataa aataaaactg aatataaata attttctgaa aaatacttgt
     8041 cctaaaatat ataatgtgct ataatataat caaataaaag aaaggggtga ttccagtggg
     8101 aacggatgtg gcactgccaa atgatgaaga aaactttatt gtaatataca aaataaaatt
     8161 agatactttt agtgctagat taagtactgg aaaaactcat gagctgggat aattattgta
     8221 attacctaga ctcaaacttt tagtagtaaa atagtaatat tatatagaca aggtatctaa
     8281 tttttaaatc ttttgtatga attaaaattt ttataaaggg tatatctgat tagaatgtaa
     8341 ttagggaaac ctagttgtga attactttta gtatatacat gtgtaaagaa atatgtatat
     8401 ggtattggtt attgtgctag atgtgtatgt aaagaaatat gtgtctagtt gggtatatgt
     8461 aaagaaatat acttataatc tatgaaaaat ttttagtata aattgctaaa acattgagat
     8521 tgttttttat agaaagttag tttataccaa ctaaaaatgt gcttaagatt tgtttgatgt
     8581 ttaattataa ataaggttgt agataaaaaa ataagtaaag acaaaggttt aaaatacttg
     8641 ctttaaatat agtttgtttt gaacacttgc caaattaaaa taattaataa aatagacatc
     8701 ttctcatagt aaaatatact gtgagaggat gtttttgctt tgttcataaa cttttatgaa
     8761 aaatttgcat agtgtaacat tttaatatac tatatatata ataaaatata gataaaatta
     8821 aatgattcaa aagagaaagg tgaaaagaga tgtattatat aggtgtagac ataggaggaa
     8881 caggtataca agcaggagta gttgataact atgggaaaat aatttttagg tcagagtgta
     8941 aaacagtaat tgaaaaagga tttgaaggaa tacttaatga cataaaaata atgatttata
     9001 agttgttgga agataataaa cttactatga gtgacataaa atctattgga tttggtgttc
     9061 caggttttat aaataaagaa ggattagtaa cttgtgtaaa tttaaaatgg aataaaaaag
     9121 cttttaataa agagttaaaa agaagatttc cagatgttga aattcatgga gaaaatgatg
     9181 caacagtagc agcacttgga gaggcaaagt ttggaagtat gaaaggtgct aatgttggag
     9241 tattatatac tttaggaact ggtgtaggtg gaggaatagt aattaatcaa aaagtctttt
     9301 ctggagcaca tggattgggt tcagaaatag gtcatcaaat tataggagaa aactatttta
     9361 attgtaattg tggaaataat ggttgtgtag aaactttttg ttcagcaact gcaattataa
     9421 agtattctca aaaactgata gaagaaggag aaaagagtag gattttagat ttggcagagg
     9481 gaaatttaga aaatgtaaat gcaaagatgg tgtttgatgc atatagagaa aatgatttag
     9541 tggcaattaa agtaataaat agatttaaag agtatttagc aaaaactttt gcaaacacaa
     9601 taaattcttt agacccagaa attatatcta taggaggagg catttctaaa tctagcgata
     9661 taatacttga tggaatagaa gatttggtaa gaaaatttgt attatacaaa acagaagata
     9721 tagctacaat tacatgtgca actttaggtt cagacgcagg aattatagga gcagcttttt
     9781 tataatgtaa atgaactgct tattggaggc gtgatgatat atgggtaaaa caaatgctag
     9841 aagaacgctt tttttgatag caataggttc aggaactatg ctgaatcctc ttaattcttc
     9901 tatgatttca cttgctttac atagtataca aaatgatttt catattagtt ttacaactgt
     9961 atcttggatt gtttcagcat tttacctagc tagtgctatt gcacaaccag tttcaggtaa
    10021 gataggagat ttaataggta gaaaggtctt atttttgagt ggtttgatgc ttgtaatttt
    10081 atcagcatcc atagtaccac ttgttcaatc attctttata ttgattttta taagagtaat
    10141 tcaagcaatt ggaacaagta ccctatatcc atctggagtt gctcttatac aaaataatat
    10201 aaaagaaaga caatcttctg cattagcagt tctaaccatt tttgcttcaa ctacagcagg
    10261 attaggtccg acacttggag gattattgtt ggacttagga ggatggcatg ctatatttct
    10321 tgtaaatata cctgttgttt tggtttctat atgcctaggg tattttttat tcccaaaaga
    10381 agtaaaagaa aagaaatcta taaaagaaac tttaaaaaat gttttgtcaa gattagatat
    10441 tattggcata cttctatttt caattgcaat gatttttata ttactatttt tgctatctat
    10501 gaaggaatct tttaacttag aacaacttgt atttggtata atactgatgt gtctttttat
    10561 ttggcatgaa ttgagaacaa aattgccatt tatagatatt aagctttttg taagtaatcc
    10621 taaattgtca aaagtttact tgcaatttat tatattaaat ttctttaatt atattttgtt
    10681 ttttggtctt cctagttatt ttcaagatgc cttacattat agtgcaaagt cgactggatt
    10741 atttatgcta tttatgtctg gtataggtat atttatatct ccattaactg gaaaatggat
    10801 agataaatct ggaactagat ttcctcttgt tacatcatca atctttatgt ttattagtgc
    10861 agtacttctt tcagtagttt ttatcaatat acctgtaata ggaaagggta ttatattatc
    10921 attagcaggt attagttatg gagttggtaa tgtagccctg caatcctcta tgtttgaaga
    10981 aagtcctcgt gacagtatag ggacagcttc tggtttgttt caaactagtc gttatttagg
    11041 ttctatttta gcaactgttt tacttggaat ggtatttgaa gtaaatataa catcagaaca
    11101 atttcagata ttaggttatg ttatggtggt cttaggtaca ataagttttt tattaaattt
    11161 tatattaaag aaagaattaa gaaaagtaga atagtggatt tatggattta ataagtctaa
    11221 taactgataa gtttaaaaat attgtttgta aaacataact ggtttgatat ttattaattc
    11281 taagttttga agcatagagt actcgataag tatacaatat tttgaattaa aattaagcag
    11341 aaatttgaac tagtctttca aatctctgct taatttagtg gttaaatttt tttatagaga
    11401 aaataagatt tgacacttag ataaaaaatc attgcatttt caaaattctt taaatcaaac
    11461 ccaagtatct cattaatctt ttccaatcta tatactagcg tgtttctatg taaaaacata
    11521 ctagtagatg tcttagaaat atttaaatca t
//
LOCUS       DAEMHZ010000129        11479 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000129 DAEMHZ010000000
VERSION     DAEMHZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.129"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..583
                     /locus_tag="KPK72_003072"
     CDS             164..583
                     /locus_tag="KPK72_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490266.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            608..5665
                     /locus_tag="KPK72_003073"
     CDS             608..5665
                     /locus_tag="KPK72_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG2490267.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFENLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(5850..6587)
                     /locus_tag="KPK72_003074"
     CDS             complement(5850..6587)
                     /locus_tag="KPK72_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490268.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6606..7541)
                     /locus_tag="KPK72_003075"
     CDS             complement(6606..7541)
                     /locus_tag="KPK72_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2490269.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7749..10325
                     /locus_tag="KPK72_003076"
     CDS             7749..10325
                     /locus_tag="KPK72_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2490270.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILTLIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            10337..11008
                     /locus_tag="KPK72_003077"
     CDS             10337..11008
                     /locus_tag="KPK72_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490271.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLKERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            11035..11376
                     /locus_tag="KPK72_003078"
     CDS             11035..11376
                     /locus_tag="KPK72_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490272.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIVNIKNQN
                     EKVILNKSIKK"
BASE COUNT         4459 a         1483 c         1677 g         3860 t
ORIGIN      
        1 aagttatctt ttattgcttt tttagacttt aagatagctt tttttctatt ttttgtaaaa
       61 taattccata attcacatta ataattcaca aaatgatata atataacaaa tagataaatt
      121 ttaaatttct aaatacataa ttaatatttt gcgaggtgat tttatgcaaa tttattttag
      181 agttaaggct gttggcaaac gcaaacctat gctagaactt accccttttg atgtatctga
      241 caacatcaat tgcctaaaag aagctatctc tgaaattgta tctaaaaatg ttcttgaata
      301 taatgagaaa atcccagaaa aatcaataat aaacttctta actaatgaag aaattgaatc
      361 tcaagctgat attggaaaag ttggatttgg ctctatttac aatgagaata aacaatctgt
      421 agaaaaggca atagaagtag cgttacaggc atttgaagat ggaatctaca aagtgcttat
      481 aaatgaagat gttattgaaa aactcgattc ccctataaat ctgaaaagtg gagatatatt
      541 tacttttata agacttacct tgcttgctgg aagaatgttt taaaataaat aatttggagg
      601 tacataaatg ttagaacgta ctagagtaga aaaaaatcct aaaataattg aaaattttaa
      661 aaataatttt actaaatctt cttcaaaaca gtactacgac ttagcaaagt ctctttggga
      721 ttgcatagaa aatagttcat actattatag agaacctgaa gacaaattct taaatatgat
      781 aaagaaatac tacaattgcc actactacga aattaaaaat atggacgagc tcctttctgg
      841 acctttaaaa aatgcattag aattaatatt tacttctgaa caagtagaag aactttgcca
      901 attaattaaa agaaagatgg attatacata ttcaggagga tattatcgta aaggttatcg
      961 ctcaaataaa cttgtggatt attttagagg tattataagg tttatttcaa gctatgttga
     1021 tttaatttat tatgattttt cagttataga ttatcttact cttccaaata aggtcttgta
     1081 ctatgactct ataatgagtg ataaaatagc actagaaata gacaaaggaa atcaaaaagt
     1141 aataagtgcc ttagaagaag taatatttgg agaaaatcaa actgctctac ttacaagaca
     1201 aatgataatt ggtatcatga aaagtcatag tgataaatgc tatgaaatgc tttctaaact
     1261 tcttttggca gccaaacaac aagaaggact tagacagcaa attgtagaga gtttagatga
     1321 gggtacaatt tctgcatcaa tatacatgtt aaaaactatt ttggacaaca atttagagcg
     1381 atttagttct gttataagag ccttggatac atggacagga cttggttttt cagaccaaaa
     1441 acctggaata atacgtaaat gtttagaact ttcatataaa tcattgactg atgaaaagtt
     1501 ccgtatagaa tcaattaata gcaatgataa tcttgaaatt tattttgcct tatgggctac
     1561 tgccacttac aacatatatg actcttactc tcttattgaa aacttacttt cttgtcagga
     1621 aaaatataaa aaacttgtag ctctttactt tttatcgcat acagaaagta catattttaa
     1681 acattctata gcatctaaaa acattaaggg tgatgattta gagatactag catgggtaat
     1741 tacaaatttc tatgtagaag gttctgttgg ctactactat agttggcaaa acagagaaac
     1801 taattttgac ttgtcaaaca ctcctattga caaagacttt gatgctagaa aagaacaatt
     1861 tgaaaattta aaacatactt tagaaatttt aaattcaaag acaaaatcat tttcaggaag
     1921 tgtatttcca tggacttgga ttgaaatttc ttctcatgaa atacttaaat gtatgatgac
     1981 tttaacagca tacgatatga atgctcaatt tatagatgtg cttatggaaa attgtcatct
     2041 tatgagttca gatttaagag cttcacttat agtcaatttt gtaaatgaac caaaaacaga
     2101 tagacaacgt gaatttttat tggaatgttt aagtgataag agtgtttcca atagagaaac
     2161 tgtattgagt ctacttagaa agcttgagct aacagatgta gaattagata agataaccaa
     2221 tctattgtct ttaaagacaa gctctattag acaaacagta ataaaaattt tacttgaaca
     2281 agatgattca aacttggaaa atattgcttc ttcactgata aaatcttcaa attctcaaaa
     2341 atgccttgga ggtcttgaaa ttgtaaactt attaaaagac aatactgcta gacaagaact
     2401 atataataag cttgttttac ttatagaaga tattgataaa tctaaaaaga atacatctca
     2461 agttcaaata ctaattgata atttgttagg taaagcaaaa aatgattact gcaaagaaaa
     2521 tggttttgga atatataacc ctgaaaacaa agttcaaatt ccagaacaat ttgattttaa
     2581 agatgattct aatagctcat caaagcttaa taaaattatc tcaaatataa gtttatctac
     2641 tcgtgaggat tctgtttctg tagcaacaaa aaaagaagca aaaaaaattc tcgatacgga
     2701 tataaataaa ataataaaag tttatgaaaa atttgatgag cttattactg aaaatgcaga
     2761 ttatgaatat gaagttgaaa gatggtcagg taatgtagac aaagtacttt taggttattc
     2821 tacttataac ttgactccta ttaaatataa agtttctaat gatactcttg ataattatcc
     2881 attagcagag ctttggagag aaactagtaa aaaacttgac ttagattaca aattaatact
     2941 tgatatctta ttttatagta taagtacatg tggatataat gaaacagaaa aaagttggtt
     3001 ttcatctata tattcaaatg tccttgactc taaaaaacaa aataaaatat atacaaaact
     3061 tgaaaaactt aaatttaatg gacttacaag aaaactttta agagttttac taaatgagtt
     3121 tgaccataaa gaaatattta actattgttt agaaatatac tataaactta gaaacttatt
     3181 gactatagaa caatgtaaaa aacaaagagt tgaatttact aaaaactatt acagacaaaa
     3241 tgaatactgc cctgctttaa atgcccctat tttaaactat tggcttgata aagcacattc
     3301 gtttatttat gatgatattt cttttactaa gtttttctac actacgtttt caaattatca
     3361 gttgctagaa tataagtttc ctttctatac tagcataaat gtgtttgctc gtgcacatga
     3421 attaggatta atgactgaag agcaagttaa tagagaaatg ataagtggat acaacgcatc
     3481 aaatataata agaacattga caaatcctag atgggctggc gacacattag aaaagtaccc
     3541 ttggcttaaa gataatttaa aaactgttac aaaccgaatt atagaaattg aatcacgtag
     3601 aggtgatatg ccaacagaag tgtctcatct agctaataat atacaatact ttgaaggtat
     3661 aaattatttt gtaaatattt tggtcgctct tggtaaagaa actttccaga gaggatattc
     3721 atatggtagc aattattcaa aaaaagatgt tttaagttct ctacttaaaa attgctatcc
     3781 tactaaagaa gatgattcaa aaaaattaaa aagtttactt aaaaataccg acattactga
     3841 gttaagattg atagaatctg ctatgtatgc ccctcaatgg gtaaacataa ttgaaaaata
     3901 tttaaactgg aagggtctga aaagtacaat ttggtacttc catgcacata taaatgaagg
     3961 cttctctgca caaaaagaat cagaggttgc tctattctcc cctatttcac cacgtagttt
     4021 cattgatggt gcatttgatg tagattggtt tttagattca tataaaacta ttggggaaga
     4081 aagattcaat atactttaca agtcagctaa atatataact agtggtggta actctcatag
     4141 acgtgcacaa ctctatgctg atgctacact tggtaagctt gataaagaca tgttgatgaa
     4201 agaaattgaa acaaatagaa atcaagataa attgagaagt ttctctttac ttcctatgaa
     4261 aaaaggagac ctaaaagaag caactttccg ttatgaattt attcaaaagt ttgctaaaga
     4321 aagcaagcaa tttggtgcac aacgtcgtga aagtgaatca aaagcttgta ctatagcgct
     4381 agaaaatcta gctagaagtg ctggatttgg agatgtaaat cgtatgatgt gggctcttga
     4441 gactgaaaaa ctaaatgaag ttgctcatct atttaaacca aaagatattg atggtacttc
     4501 aatttggata gatgttgatg aaaacggaaa agcatctact aaagttcaaa aaggcaacaa
     4561 gtctttaaag agtataccaa aagctattgc taaacatcca tatgtacttg aacttaaatc
     4621 tgtccaaaaa gatttaaaga gtcagtattc tcgtgcaaaa cagagtttag aacaagctat
     4681 gaaaaatgaa atagtattta cagttaaaga aattgccaat atctataaaa atccagttat
     4741 ctgccctttg attaaaaatc tagtatgggt atgtgatgat aatattggat atgtttataa
     4801 tgataagtct aagttcttta tttgcaatat agaaggtgaa acttataagt tgacggcaaa
     4861 aaataaatta aaacttgcac atccagttca tttgtttgaa tctggtaagt ggagtgagtt
     4921 ccaacatgat ttatttagta agcaaattgt tcaaccattt aagcaagtat tccgtgagta
     4981 ttatagaata aatagtgatg aattatctga aaaaactata tctcgcagat atgctggtca
     5041 ccaaattcag ccaaagaaaa ctgtagcact tcttagaaat cagctttgga ctgtcgatta
     5101 tgaagaggga ttacagaaag tattttataa agaaaatata gtggcaaaga tgtttgcact
     5161 tgcagattgg ttttcaccag cagatataga gcctcctact attgaaacag tagaattttt
     5221 tgatagaaaa acttataaaa gtttagaatt agcttctata cctccaattc tattctcaga
     5281 agttatgcgt gatatagacc ttgttgtaag tgtcgctcat gctggtggtg ttgaccctga
     5341 aactagccat tctactgtag agatgcgtat agcaattgct aaagaacttt tattattatt
     5401 aaaaattaac aatgtcaatt ttgaaggctc tcatgctctt atagcaggta atttaggtga
     5461 gtattctgtg catatgggtt ctggtattgt acataaaaaa gggatgggtg ctttgaatat
     5521 acttcctata cattctcaaa gtagaggtaa gatattcctt ccattcgctg atgaagaccc
     5581 taaaactgct gagattatgt ctaagatact attacttgct gaagatagta agattaaaga
     5641 ccctagtatc ttaagtaatt tataagtata aaaactatct ctaaaatata ttggactttc
     5701 ttaaaaaaat tagaaagtcc aatatatcct atactgtaat ttatatagat taaatcataa
     5761 ttacaaataa tttttatata aacttctctt atacattatc tttacggtac ttattatatc
     5821 acacaaaaag tatttttctt ctatcttcac tagaatcttt taaccctata aaatctatat
     5881 aaaaaatctt tttctagaga tttgtttaat atggcatttt cttgaaaatt tataaacctt
     5941 atctctaatg aagatttatt tataggtata accttatgta taaatcttag atttactatg
     6001 tattgtctat gggtcataaa gaaattagga ggtaggcgtt tttttaaatc atcaaagttc
     6061 tcataaaact ttattatatc ttcatcttta gtgtgtagat taattgctct tgaatttttc
     6121 tcaaacataa ctatatcatc aaaattaatc atatgtatct gcttccttat tttatataca
     6181 aaaagggtgt cttctataaa attattatta gcaatcttat gggagttaat atggtctatt
     6241 gctatgttaa ttgaatcaag tattttttct tttttaaatg gtttaactac aaaatctata
     6301 ggatgcactt taaagctttc aagactataa tcagcgaaac ccgtcacaaa tactatatgt
     6361 atatccttat ctatagaaag aatttcttca gcagaatcaa tcccattttt atatggcata
     6421 tcaatgtcaa taatcaataa atctattttc tcatttttta taaattcaat tgcctctata
     6481 ccatccttaa cttttttaac ataatctaca tatccagact ctttagctat atttgctata
     6541 tcatctctta caatcttatc atcatcacaa atcaaaactt ttatcataga cttttctcct
     6601 cctaactaat aatattttgg gattaagatt acaaattcag ttaaaaaatc atcactattt
     6661 acaagtatac tataatcata cttatctaat atatttttaa ctaaatgtag cccatatccc
     6721 ctatcttgtc cttctttagt actaaatcca ttccaaaata tactttcaac attatgtatt
     6781 ttaggaccat tattttttat agctataata tactgaaatt catcttcata tgcatgtaat
     6841 cttatatact tcttttcttt ttttatagta gacaaagact ctattgcatt atctattatg
     6901 tttaatagta ctgaactcat atcattaggt tcaatctcta aattatccat aaaatcttga
     6961 atgtagacat caaaagatat atccttttca agacattcct cgtattttaa tgaagctata
     7021 gcatctaaat aagatacttt tccatacctt gaattttttg atttaaagct atttgatata
     7081 ttcattatat attctttggc atcttcatct agcccagtat ttaatagaga atacactact
     7141 tgcaaatgtt tcaaatagtc atgtttttga cttcttaatt tgataattat attatcctta
     7201 tgatttattt tactatctag tttttgtagt ttatattcct gttcattatt attatttatc
     7261 aatcttataa ctttaaagcc tgtataaaaa cttaaaataa ttaaaaccaa aaaaagtata
     7321 atatttaata ctccttcaag taaaaaacta tatcctaacc ttaattgtat atatttagtg
     7381 ccctccataa ataaaatcaa tgcaaaacac gatataaaaa atgttaatat gcctcttttt
     7441 aatatgtcta attctttgta aaatttatac ttattcatta taagattgct aaatataaac
     7501 aaagttagta ttactatagg aaatacaaaa ttgttatcca tgattcccct cccctctaca
     7561 atttatatag ggattatatc attttaaaaa aattatgtaa aaagttaaaa tttacatata
     7621 tattaattaa acttgtaaaa atacagatga atttgtacaa ttaactactt aaaatttgaa
     7681 aatacttact ttgctatata ttttagataa atatataatt gatttagtta agaaaggaag
     7741 tggtaaatat gaaagataat ttgaaaatag tactaaggta tataaaaagt tataaagcta
     7801 ggtctttagc aattgttgtg agtataattc ttgctacatc actcgttgtt ggtattggta
     7861 ctctctcaag aagtgctcag caagcagatg tagatagatt gaaaagagag cttggaactg
     7921 accatgtata ctttaaaaat ataaagaaaa atcaattaaa gtccataaaa tctagcaaag
     7981 atattaaaaa tctgggtata acatcttact atgcgtctac agatgttaat gaaaaattac
     8041 ctataaacat actatatgca aataaaaatt atttgactag tcattctaaa cttataaaag
     8101 gtcgatttcc aaaagctagc aatgaggtgg tagtcgagaa atggatttta aatagtatgg
     8161 gcttaaaacc agagataaat caaaacctta cttttaaatt atatcaaaaa gaaaatccag
     8221 aaacttttaa agttgttgga atccttgaag atagatttat agagaaaaaa aagggacaat
     8281 gtgaaatttt ttctaatctc aatgaatcag aactgaatga ttttacaact tatattgaat
     8341 ttaatgaagg cagtgatata aatacaaaca tagacaatat aataaaaaat gctatgttag
     8401 atgagaaaag tataggaaga aactcaatgc ttatagaatc tactatgaaa aacggtactt
     8461 tagataattc aagtaaatac gttgcaattg ctatgagtct atttgctggt attgtaatat
     8521 atagtatcta tgttatatca atataccaaa gagtacagga atatggcgtc ttaagagcta
     8581 ttggttccac caactttagg attttcaaac ttatgcttta tgaacttttc atacttactc
     8641 taatagcaat gcctataggt atatgtatag gtatgggagg tgcacaaata tttaataggt
     8701 ctgtaggaaa tattcaattt gaaggaaata taaatgttac accttttgtt attccagata
     8761 aaataattct cttatcaatt gcatgtacaa tactaataat actaattata agtttcttta
     8821 catacttaaa aataagaaga atttctccta ttgatgctat cagaaaaact tttggagctg
     8881 ataaaaatgt aaccaaagtt aattatttaa tatcaaaatt aactttaaat atgtcagtaa
     8941 caaaatctat atctgcgaaa aacatcttta gaaataaaaa ggggttcatt ataattgtat
     9001 tatctatgag tataggaggt ataatcgtca taaaagacaa ttataaatat tcttttttag
     9061 atgtgcaaaa caaatctact caagaacaaa catatatgaa tgcagatttt attctatcaa
     9121 atgtttatct caatgaaatt ggtaacaata cttcttttaa agatataaaa gggtttaatg
     9181 ataatcagat agataaaata aaaaatatag atggaattga caaagtcaaa actgctagta
     9241 tcttaaacac aagaatagag gtaaataaaa taaatagatt agattactac gaagctataa
     9301 attcaactcc ttattataag ctaaatcctc tgttcataaa aaataagact actggcaaat
     9361 atacactaaa gcaaaagcta aaaggttata atgatgagat gataaagtca ttagaaaaat
     9421 acttagtaag tggaaatata aacttgaaaa aaatgaaaaa agaaaattta gctattgtat
     9481 atgtaccaga aatttctaaa acacagaaat acacaaagac ttttactcct ggtgaaggaa
     9541 caccagctgt agatataaaa gtaggtgata ctataaaagt caaatatcca aagggtggga
     9601 tggatacagg tctttatgaa agtctaaaag ataattatga atacttagaa tatgaattta
     9661 aagttggagc tattataagt tatccttttg cagataatgg tatgtactct ggagatgatg
     9721 gtgtagatgt aataacaagt agcgaatacc taaaaaaatt aactggtgta gataagtata
     9781 acttagttta tgcagacctt aatgaaaatg caaacaagaa aaaaataaat aaccttttag
     9841 gtaaaattgg aagtgaagtt cctggaacta caacagtaga tatgatggta gaaaaagaaa
     9901 attctgataa gatgacatct agaactatga tttatagtta tggtatagta gcaataatgt
     9961 tcataatcag tgtacttaac ataataaaca acataagtta taacttaact tcaagaacta
    10021 gtgaatttgg aatgttaaga gctattggta tctctgagag aggctttaaa aatatgatac
    10081 tttatgaagg aatattatat ggtgtactct caagtataat tactatcgtg tcaggaatta
    10141 taatacaatt taaaatgtat tatatgcagg gctttgtatc atatggattg ggattttcaa
    10201 tagattacaa aatatatata ctagttgttg tagcaaatat aattgtaggt attctagcta
    10261 catatattcc atcaagaaag ataaataaaa ttagtatagt tgaagctata aacattacag
    10321 aataggagga ataattatgc ctatattgga aactattaac ttaggtaaga tttatggaaa
    10381 aaaggaaaca tctgtacatg cactaaagaa tgcaaattta aaaataaata aaggtgaatt
    10441 tgttgcaata attgggccaa gtggaagtgg gaaaagtaca tttctacatt tagtaggtgg
    10501 tcttgagaga ccaagtaatg gtactataaa agttgctggt aaagatatat gttgcctttc
    10561 agataaagaa ctagcaagat atagaagaca aaaagtagga tttgtctttc aacagtataa
    10621 cttaattcct gtattaaatg taaaggaaaa tatagaatta ccactaaagt tagacaataa
    10681 aaaaatagat aaagaatata ttgaagacct tataaattta ttggggctta aggaaaggaa
    10741 aaatcatctt cccaaccaat tatctggtgg tcaacagcaa agagttgcta ttgcacgtgc
    10801 tttatctgca aaaccaagta taatcctagc agatgaacca acaggaaatt tagacagtaa
    10861 aactacagaa gaagtcatgg atttactaaa aagttctata aaaaaatata accaaaccct
    10921 tataataata acacataatg aaaatattgc tagaaaagca gatagaatta tatctataat
    10981 agatggagag cttaaattaa cactctaaaa ctatttatag aaaagaggat ttaaatgtta
    11041 ttgattttag gtaatttaaa tttaaacttg gattttgact acagaataat aagagaagaa
    11101 aatgatgatg tagatatatt tatagatatt aattatcgta gcttagatat agatactgat
    11161 ggttctaact tatttaattc tagaatacaa tttccatttg taagagcatt gattctaagg
    11221 ctaaataaga acaatcaatg tatgacaatt catttgttaa gagatataga tttattctct
    11281 gcttttgcca actttgaagt ggattatact gactctatag taaatataaa aaatcaaaat
    11341 gagaaagtaa ttttaaataa atctatcaaa aaatagtaag ggggccttaa taggccccaa
    11401 tttttttctt ttctaaactt attatatcat gataaataaa ttagtcaata gaattttctg
    11461 aatattttta taatttttt
//
LOCUS       DAEMHZ010000130        11397 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000130 DAEMHZ010000000
VERSION     DAEMHZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.130"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..709
                     /locus_tag="KPK72_003079"
     CDS             <1..709
                     /locus_tag="KPK72_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490273.1"
                     /translation="KVAICEDEKVHQELLIEYLQKLFNELSIDYDLCIFNSGEQLLEN
                     YPEDMDIFLLDIQMGELSGMDIARKIRDIDSNTVEIIFTTSLIEYIQEGYEVRAYRYL
                     LKPIEFNDIKNHITACIEEIHNRRDKYLVISNKYENYKIKIENITYIEVQNKDITIHT
                     LDNDYEIKMSMNKIEKELKKYNFIRCHKSFLININFVENVKQYVAILEDGQEIPISRY
                     KFKEFKTIFLKSLRKVL"
     gene            928..2049
                     /locus_tag="KPK72_003080"
     CDS             928..2049
                     /locus_tag="KPK72_003080"
                     /inference="COORDINATES: protein motif:HMM:NF025854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2490274.1"
                     /translation="MFLYKLTFEISYKDSFLYAFSFAFLYSIIGRIIYTFLINFLFDK
                     VVEYSALGYHSLISIILNVILILLLKYFKQIINAELDIKYYIYVGLTVIINGISILFL
                     FISGEYINDLYKIMEYSDAIPRMSVIPYIQTIQSIIPAIMVFCNIFLIIIINNLIKGM
                     KAKSELESINNKLNMQYNYYLNIQESHMKVRKIYHDINNHLACIKKIENGDVDNYIKS
                     INEELRDFESSFNTGNKILDIILNEKSIDCRNNNIKLLCDINLRKCEFIQMIDISSIF
                     SNILDNAIEACEKVRQGRYIKLRGVIVKSYFIITCENSKNDKLRFKNNILITSKDDKF
                     FHGIGLKSIKSSIKKYDGELEIIEGKDNFTLNIYIPIQN"
     gene            2209..2793
                     /locus_tag="KPK72_003081"
     CDS             2209..2793
                     /locus_tag="KPK72_003081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490275.1"
                     /translation="MKKFKLYGNCINKNIKLIESKKKILLILFVVSLLLCVCSFFIKS
                     LITGAPRDNLIRIYNILGTSFDRMIEQENFKNKDVYKEELSKLLNTENFKNVSKIEVY
                     LNQNPKNHNKKSMNLMYSYENRLKKNTWEAFISSKLRTYDGKEIYCHIVCEEPKYEAY
                     KLPVNIICILSLVVASIFLILYLYMIVKFHKIGR"
     gene            2798..5437
                     /locus_tag="KPK72_003082"
     CDS             2798..5437
                     /locus_tag="KPK72_003082"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2490276.1"
                     /translation="MKMIFVLLRGYAIKNRKKFLIMIFAISVSTTIIFGTTIARLSQS
                     KYVSDEIYKQSPSYQIEVGNISEEDFETIIKDNNVKSSVLRKYYGNFSYNEKFYVLEE
                     YNKVSFKKLKHTLNNGRFPKQKDEIMISNNLFKELKSQNKLKLIDTHSHKKEYEVELS
                     YSKEYMDNLKEHQIFNQTHKFKIVGTYNMTSIMENSSEGIGIYVYKDFEYPEQLTTYN
                     GFIDLKTGFKNVQTEIDKLSIKLDSGELNIKSNKTLATAKEDSQSALGGFDLFDTGTI
                     VASICIIFNIFNIMMKELVQEIGLLRVVGMSKRQSLFIFMLKNILVLLVGSLIGFLGG
                     YLLAEVMIKYFNLTSGFIDVSDAPIYVSKSAVFKTLKLTISMLLISTILPILITLKSY
                     PVNMMSGRLRNPINIFDEIFNKIKIYGKLKILGLNFLKKIRNLSKLERKLFFRDNVDW
                     NIALNNSKRNSIYILTTAIIVGMAGLYGVRQFITSQQVSSIGDPLLQRLGDYSIDLSY
                     NGISKLDDSLISQQDLDNISSIDGVSDVYKFNSTTAYAKIKTKDLSSAYRKNLSIKDD
                     NREIEMKFDFIGLNKDALSKYDSFLDSGQLYKKNNNVIEAVVSNYFLDQTYTHSQQKY
                     SEKLKIGDTLKFKVLSEENGVKKYNNIEIKIVGFLSEEWFTKGTYTNSQTPDIIVDSS
                     DYKKITGDNNFSQVKIKVEEQDLENIKLSIENILKDKKYIKYSDKNTINQEQGSLAWQ
                     TIIRNIVNSAMLSITALTNIVFSVVTSITMRKKELGLMRAIGLSIKDLKRILMYEGLI
                     YGVISSTVGVLFILYKGISWVHLLKMTAKFQGIPYEGPLFIFPVIPTLVFILSTIIIS
                     LISVTFTFKIINKYSIVNQIREN"
     gene            5465..6130
                     /locus_tag="KPK72_003083"
     CDS             5465..6130
                     /locus_tag="KPK72_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490277.1"
                     /translation="MRNLSMKGIRKIYGENENKVNALNGIDLEINPHKFTAIIGASGS
                     GKSTLLHCMAGLDKPTSGHIYLDDIDIYTMDDTKLSRIRREEFGFIFQSFNLIPVIKV
                     YENIIMPVSLGCEKIDKKYIDMLIDKLGLREHINKYPNQLSGGQQQRVAIARALANKP
                     SVIFADEPTGNLDSETTIEVMDLLKTCVKEFKQTLVMITHNNDIASMADKIITIKDGM
                     IYE"
     gene            6542..6721
                     /locus_tag="KPK72_003084"
     CDS             6542..6721
                     /locus_tag="KPK72_003084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490278.1"
                     /translation="MKDKFSVKKINDKIEIYDMEINASNPCKAFWEENGKTGQYDCNY
                     DCGIFNNSPEASTSY"
     gene            6950..8212
                     /locus_tag="KPK72_003085"
     CDS             6950..8212
                     /locus_tag="KPK72_003085"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2490279.1"
                     /translation="MNLEYHNKVIRYKKNTCYIYDKVSKKHMFISKEVFNYLKKAEEY
                     DLNISKFIDGFEDTDDKEYMKLVISNLKKIGFIKNYEYERRFINTENNNFESIYIMVT
                     DKCNLNCRHCTTNCSPDGENILSTQNIKDIIYNISLLNPKNIIFTGGEPLIRKDIEEI
                     VDFAKQNIKDSSFSLSTNSTLISNENVSMIKRLFDHVDISIDGVDELSCSKIRGKGVF
                     GKVLNSIKLLQENGLHKISLSMVFSSSNEHLRDDFIELNKSLKTIPIERAFIPEGRGY
                     DNILDFEYENIHIPKSIPSIYNIENKYEKLSRNISSCSCNAFKNNLFIDSNGDVYPCP
                     SLIKSNYKITNLAYKKYTKQQIIDMIRMRSIDFSESLEYRDSKCEKCDLNIFCWDCPA
                     NFERAKEYGEIDNWCNLMKKNLDNIVWR"
     gene            8222..9256
                     /locus_tag="KPK72_003086"
     CDS             8222..9256
                     /locus_tag="KPK72_003086"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2490280.1"
                     /translation="MNFNIIVTSKCNLRCNYCYEGNEKAQKDMSINLANKTVEFIINT
                     LNKLDDIDRRNRVVFHGGEPMLNFELIKFIKTKLEKELEKKYIIDYMTTINGTIINDK
                     IIDFIKKNNISLSISIDGMKSIHDENRVYHDGTGSYNIMKKNLYLLKENGIIPRARMT
                     YQSSNIDKLYENIVDISKLGFEVCVPVADFYDTGWSDEKITLLENQIDKIISDKNLND
                     MRVSIVNKNILCRQKSDCFGGFTTITISENGDLYPCLFTLGERKFIIGNILNGNGINQ
                     DKLKSIFKEIANEPNECNNCNANDLCFGNQCKFLNYMVTGKYNVAPSINCRLTGVELN
                     TYKKLCKGSE"
     gene            9258..10496
                     /locus_tag="KPK72_003087"
     CDS             9258..10496
                     /locus_tag="KPK72_003087"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2490281.1"
                     /translation="MNYLNKYYVKDLANISIDKKEVYISAKFKPEWFKFPKICLDILD
                     YSFDKQITVGELLELFNDENDKIYFKKILSNLDKIGILMSKPISNVYVSRLNKVTFAI
                     TNKCNLKCSFCSQNSKIDNQEELSFEKIKVIIDKAMIFNPLKVVLTGGEPMIRNDFFD
                     IVDYIKSNYQVDIQLCTNGTLIDNFNINKFKNSINAVDLSLDGFDEPSCSSYRGKGVY
                     EKVVNSIDLLKIIGIHNISASMVVGNHNIDIVDKFVSFCNSKSIIPIKREFMSIGRGK
                     EAIKYLKDEKDSMFYPDYNKIDKKIGARTCSTGITQLYIDHKGDIYPCSLLDSDEYKI
                     CSAMDLDNNIVDKVYKKEMDVYKKLAELSPLNYHKCKGCKYKLFCNNCIANMKIMIES
                     DKEFEHNCQKLKNIYEKCLN"
     gene            10535..>11397
                     /locus_tag="KPK72_003088"
     CDS             10535..>11397
                     /locus_tag="KPK72_003088"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2490282.1"
                     /translation="MNNVKVAVLDTGIDMEDSEIKKFCKFDKNLQIQSINECIDDLNG
                     HGTFVSKTIINICKNIDIYPIKIFNKFGRTNSLNAILVLRKILNSEIDIINISASTFD
                     YSYENEFRDICNKLKSKNKIVICSKHNSREVNNSIPTIFDSVIGVEGHRNIYENSQYV
                     YKKNNKIQMLANNKESFVKFKGSIIPFGKNSKACAVATGIIANILKYNPGIYFNQLEE
                     ILLSNSLDENKLCTSNLLDIKSYNNLNQEHVLYINKGILEIINNNFAVEKVDFQMIQE
                     YGIMNTITNIG"
BASE COUNT         4830 a          986 c         1754 g         3827 t
ORIGIN      
        1 taaagtagca atttgtgaag atgaaaaagt acatcaagaa ttattaatag aatatttgca
       61 aaagttattt aatgaattgt caattgatta tgacctctgt atattcaatt caggagaaca
      121 attattagaa aactatccag aagatatgga tatattcttg cttgatatac aaatgggtga
      181 attaagtggt atggatatag caagaaagat aagagacatt gatagtaata ctgtagagat
      241 aatatttaca acttcactta tagaatatat acaagaaggg tatgaagtaa gagcatatag
      301 atacttacta aagcctatcg aatttaatga tattaaaaat catatcactg cttgtataga
      361 ggagatacac aacagaagag ataagtactt ggttattagt aacaaatatg agaattataa
      421 aattaaaata gaaaatataa cttatataga agttcaaaat aaagatatta ctatacatac
      481 attggacaat gattatgaaa taaaaatgag catgaataaa atagagaaag agctcaaaaa
      541 atataatttc attagatgtc acaagagttt tttgattaac ataaattttg tagaaaacgt
      601 aaaacagtat gtagctatac ttgaagatgg tcaagaaata ccaataagtc ggtataagtt
      661 taaggagttt aagacaatat ttttgaagtc tttaaggaag gtactataat gaatgaagca
      721 ctaattttaa atataattga tataataaat tctatactgt atttagtaat tttttattta
      781 atgcttacta aaattaaagc tagaaaaaca agtataaaaa aaataaatta tattataatc
      841 tttgtcctta ctatattaat attaatctca cctaaattta aggatattgt aataataaaa
      901 tataatatcc ttgaggggtt tttattaatg tttctataca agcttacatt tgaaatttct
      961 tacaaagatt cttttttata tgcttttagt tttgcttttt tatatagcat aatagggcgt
     1021 ataatttata catttttaat aaatttttta tttgataaag tagttgagta ttctgctttg
     1081 ggctatcatt ccttaatttc aataatatta aatgtgattt taatattatt gcttaagtat
     1141 tttaaacaga ttattaatgc agaattagat attaaatatt acatatatgt tgggttaact
     1201 gtaattataa atggtataag tatattattt ttatttatat caggagagta cataaatgat
     1261 ttatataaaa ttatggaata ctctgatgct attccaagaa tgagtgtaat tccttatatt
     1321 caaactattc aaagtataat accagctatc atggtatttt gtaacatatt tttaataatt
     1381 attataaata atttgataaa aggaatgaaa gctaagtcag aattagagtc aataaataac
     1441 aaattaaata tgcaatataa ttattattta aatattcaag aatctcatat gaaagttagg
     1501 aaaatatatc atgatataaa taatcattta gcatgtatca aaaaaattga aaatggagat
     1561 gtagataact atataaaaag tataaatgaa gaattaagag attttgaaag tagttttaat
     1621 acaggaaata aaatacttga tattattttg aatgagaaaa gtatagactg tagaaataat
     1681 aatataaagt tattatgtga tataaactta agaaaatgtg aatttataca gatgatagat
     1741 atatcatcta tattttccaa tatattagat aatgcaatag aagcatgtga aaaagtaaga
     1801 cagggtagat atataaaatt acgtggagtc atagtaaaat cttattttat cattacatgt
     1861 gaaaattcca aaaacgataa attaagattt aaaaataata ttctaattac tagtaaagat
     1921 gataagtttt ttcatggtat aggtttaaaa agtataaagt catctataaa gaaatatgat
     1981 ggagaattag aaataattga aggaaaggat aattttacat tgaatatata cataccaata
     2041 caaaactagt taaactacac ttcaaatatg atagttacga cagtataatt accacttggg
     2101 caaatatatt gagtaaaaaa aataaaggtg ttatctttta aacataaaag ataacacttt
     2161 tatttttttg attattttaa ataaataatt ttaaggagaa tgtttgtaat gaaaaaattt
     2221 aaattatacg gtaattgtat taataaaaat ataaaattaa ttgaaagtaa aaagaaaata
     2281 cttttaattt tgtttgtggt atctttactt ctatgtgtat gttctttttt tattaaatct
     2341 cttattacgg gagcaccaag ggataatctt attagaatat ataacatttt aggaacctca
     2401 tttgatagga tgatagagca ggagaatttt aaaaataaag atgtatataa agaagagtta
     2461 tctaaattat taaatacaga aaattttaaa aatgtaagta aaatagaagt atatcttaat
     2521 caaaatccaa agaatcacaa taaaaagagt atgaatttaa tgtactctta tgaaaataga
     2581 ttaaaaaaga atacatggga agcatttatt tcaagtaagt taagaactta tgatggaaaa
     2641 gaaatttatt gccatatagt atgtgaagaa ccaaaatatg aagcatataa attaccagtt
     2701 aatataatct gtattctttc tttagtagta gcaagtatat tcttaatatt atatttatat
     2761 atgattgtta agtttcataa aatagggagg taagaatatg aaaatgatat ttgtattatt
     2821 gagaggatat gccataaaaa atagaaaaaa gtttttaata atgatatttg ctatatcggt
     2881 ttctactact ataatttttg gaactacaat tgcaaggtta agccaatcaa aatatgtttc
     2941 cgatgaaatt tacaaacaaa gtccaagtta ccagattgaa gttggcaata taagtgaaga
     3001 agattttgaa acaataataa aagataataa tgtaaaaagt tcagtattaa gaaaatatta
     3061 tggaaatttt agttataatg aaaaatttta tgtattggaa gagtataata aagtttcttt
     3121 taaaaaatta aaacatactt taaataatgg tagatttcca aaacaaaaag atgagattat
     3181 gattagtaat aatttattta aagaactaaa atcacaaaat aaattgaaat taattgatac
     3241 acattcgcat aaaaaagaat acgaggttga attaagttac tcaaaagaat atatggataa
     3301 tttaaaagaa caccaaatat ttaatcaaac tcataaattt aaaatagttg gaacttacaa
     3361 tatgacaagt attatggaaa attcatcaga agggataggg atatatgtgt ataaagattt
     3421 tgaatatcct gagcagttga ctacatataa tggatttatt gatttaaaaa ctggttttaa
     3481 gaatgtacaa actgaaattg ataaattatc tattaagctt gattcaggag aattaaatat
     3541 aaagagtaat aagacactag ctacagcaaa agaagatagt caatctgctt tgggaggatt
     3601 tgatttattt gatacaggca ctatagtggc atctatttgt atcatattta atatatttaa
     3661 cattatgatg aaagaactag ttcaagaaat aggattatta agagttgttg gtatgagtaa
     3721 gaggcaatct ttatttatat ttatgctgaa gaatatatta gtgttattag ttggatcatt
     3781 gataggattt ttaggaggtt atttacttgc agaagtgatg ataaagtatt ttaatttaac
     3841 aagtggattt attgatgttt cagatgcacc tatttatgtg agtaaatccg cagtatttaa
     3901 aactcttaaa ttgacaatat ctatgttatt aatatcaaca attttaccaa ttttaataac
     3961 tttaaaatct tatccagtaa atatgatgtc tggtagacta agaaatccaa ttaatatttt
     4021 tgatgaaatt tttaataaaa taaagattta tggaaagtta aagattttag gcttgaattt
     4081 tttaaaaaag ataagaaatt tatctaaatt agaaagaaaa ttatttttta gagataatgt
     4141 agattggaat atagcattaa acaattcaaa aagaaatagt atctatatat taactacagc
     4201 aattattgtt ggtatggctg gactttatgg agtgaggcag tttataacat ctcaacaagt
     4261 atcaagcata ggtgatccat tacttcaaag gttaggagat tacagtatag atttatctta
     4321 caatggtatt agtaaattag atgattctct tatctcacaa caagatttag ataatatatc
     4381 ttctattgat ggagtttcag atgtttataa atttaattca acaactgctt atgctaaaat
     4441 taaaacaaaa gatttatctt cagcatatag aaagaattta tctataaagg atgataatag
     4501 agaaatagaa atgaagttcg actttatagg attaaataaa gacgctttat ctaaatatga
     4561 tagtttttta gatagtgggc agctatataa aaaaaataat aatgtaattg aggctgtagt
     4621 aagtaactat tttttagatc aaacctatac tcatagtcaa caaaagtata gtgaaaaatt
     4681 gaaaataggt gatacattaa aatttaaagt tctatcagaa gaaaatggag tgaaaaaata
     4741 taacaatata gaaataaaga ttgttggatt tttaagtgaa gagtggttta caaaaggaac
     4801 atatactaac tctcaaactc cagatataat tgtagattca agtgattata agaaaataac
     4861 tggagataat aacttttctc aagtaaaaat aaaggtagaa gaacaagatt tagaaaatat
     4921 aaaattatct attgagaata tacttaaaga taaaaaatat ataaagtata gcgataaaaa
     4981 tactataaat caagaacaag ggagtttagc atggcaaact ataattagaa acatagttaa
     5041 ttcagctatg cttagtataa ctgcattaac aaatatagtt tttagtgtag tgactagtat
     5101 aactatgagg aagaaagaat taggattgat gagagcgata ggtttatcta taaaggattt
     5161 aaagaggata ttaatgtatg aaggtttgat atatggtgta ataagttcca cagtaggtgt
     5221 attatttata ctttataaag gcattagttg ggtacattta ctaaaaatga ctgcaaagtt
     5281 tcaaggtata ccatatgagg gcccactatt tatatttcca gtaataccaa cactagtatt
     5341 tatattaagt acaattatta tatctttaat ttcagtaaca tttacattta aaattattaa
     5401 taaatatagc atagttaatc aaataagaga aaattaagtt taacataaga aggagaaaaa
     5461 ttgtatgaga aatttgagca tgaaaggtat aagaaaaata tatggagaga acgaaaataa
     5521 ggttaatgct cttaatggca tagatttaga aataaatcca cataaattca cagcaattat
     5581 aggggctagt ggctcaggta aatctacatt acttcattgt atggcagggt tagataagcc
     5641 tacctcaggt catatatatt tagatgatat agatatatat accatggatg atacgaagct
     5701 atctaggata aggagagagg aatttggatt tatatttcaa agttttaact tgattcctgt
     5761 aattaaggta tatgaaaata ttataatgcc tgtgtcattg ggttgtgaaa aaatagataa
     5821 aaaatatatt gatatgttaa tagataaact aggattaagg gaacatataa ataagtatcc
     5881 aaatcaattg tctggtggtc agcaacaaag agttgctatc gctcgagcat tagcaaataa
     5941 accttcagta atatttgcag atgagccaac aggaaattta gatagtgaaa caactataga
     6001 agttatggat ttactgaaaa cttgtgtaaa ggaatttaaa caaactttag taatgattac
     6061 acacaataat gatatagcat ctatggcaga taagattata accataaaag atgggatgat
     6121 atacgaataa ttttgtaatt aaaaatatta agaatggggt gcagttaaat ttctatgaaa
     6181 ataaatttaa ctgtattttt ttatgcaaaa ttaattgata ttatatgata taaagactta
     6241 taactttaat agaagttaat taagatgata catagattaa aataaatcgt ttttttacaa
     6301 ataaaataat taatattact tcaattaaat agttctattt ttattgacca ctaaaaaata
     6361 tatctacata aatcttgctg atatagcgca tataaagctt aaatatgaca cttacaccta
     6421 taagacaaca cttcacgcaa atgaattgat taactaaata ttagatgtta cactatcttt
     6481 aataacagat aactccggag attaatctgt aattaatatt aaaactaggg ggtttttaaa
     6541 aatgaaagat aaattttcag taaaaaaaat aaatgacaaa atagagatat atgatatgga
     6601 aattaatgca tcaaatccat gtaaagcatt ttgggaagaa aatggtaaga ctggtcaata
     6661 tgattgtaat tatgactgtg gaatattcaa caattctcca gaagcatcta catcatacta
     6721 aaaaaagtta gggcaaaagt aaagtagggg aaatggctta gggataatta ttttatattc
     6781 ctaatcattt ccctattatt ataaatgtat atcttaaaat aaagataagt atataggatt
     6841 atttcatgta actacattat tttgaataaa ttatatggct atatacagta agaatttgaa
     6901 aaaatagtaa taatatttaa ttaataataa aaatagggag aggtagataa tgaatttaga
     6961 gtatcataat aaagttatta gatacaaaaa aaatacatgc tatatttatg ataaagtttc
     7021 taaaaagcac atgtttatat ctaaggaagt attcaactat ttgaaaaaag cagaggaata
     7081 tgacttaaat atttctaagt ttatagacgg gtttgaagat actgatgata aagagtatat
     7141 gaagttagta atatcgaatc taaaaaaaat aggttttata aaaaattacg aatatgaaag
     7201 aagatttatt aatacagaaa ataataattt cgaatctata tacataatgg taactgataa
     7261 atgtaactta aattgtagac attgtacaac aaattgcagt ccagatggag aaaatatttt
     7321 atcaacacaa aatataaaag atattatata taacataagt ttactaaatc ctaagaatat
     7381 aatatttaca ggtggagagc ctctaataag aaaagatata gaagagattg tagattttgc
     7441 aaaacaaaac attaaggata gttcattttc attatcgaca aattcaacac ttatatctaa
     7501 tgaaaatgta tctatgataa aaagattatt tgatcatgtt gatataagta tagatggtgt
     7561 tgatgaatta tcttgttcca aaattagagg caaaggtgta tttgggaagg tattgaacag
     7621 tataaaatta ttacaagaaa atggattaca caaaatatca ttatcaatgg tatttagttc
     7681 aagtaatgaa cacttaagag atgattttat agaattaaat aaatctttaa aaactattcc
     7741 aatagaaagg gcctttatac ctgagggaag aggatatgac aatatactag attttgaata
     7801 tgaaaatatt catataccta aatctattcc tagtatatat aacatagaaa ataaatatga
     7861 aaagttaagt agaaatataa gttcttgttc atgtaatgct tttaagaata acttgttcat
     7921 tgattcaaat ggagatgtat atccatgtcc ttcattgatt aaaagtaact ataaaattac
     7981 aaatctagct tataaaaaat atacaaaaca acaaattatc gacatgattc gtatgagaag
     8041 tattgatttt tctgaaagtt tagaatatag ggattcaaaa tgtgagaaat gtgatttaaa
     8101 tatattttgc tgggattgtc ctgcaaattt tgagagagca aaagaatatg gtgaaataga
     8161 taattggtgt aatttaatga aaaaaaactt agataatata gtgtggagat agggggaaat
     8221 tatgaatttt aatataattg taacatcgaa atgtaattta agatgtaatt attgttatga
     8281 aggaaatgaa aaagcacaaa aagatatgag tataaattta gccaataaaa cagtagaatt
     8341 tataatcaat acattaaata aattagatga tatagataga agaaatagag tagtttttca
     8401 tggtggagaa cctatgttga attttgaact aattaaattt attaaaacca agttagagaa
     8461 agagttagaa aagaaatata taatagatta tatgactact ataaatggaa ctatcattaa
     8521 tgataaaata atagatttta taaaaaagaa taacatatca ctgtcaatta gtatcgatgg
     8581 tatgaaaagt atacatgatg aaaatagagt atatcatgat ggcacaggtt cttataatat
     8641 catgaaaaag aatttatatc tgttaaaaga aaatggaata ataccaagag caagaatgac
     8701 atatcaatca tcaaatatag ataaactata tgaaaatata gtagatatat caaaattagg
     8761 atttgaagta tgtgtacctg tagccgactt ttatgatact ggatggagtg atgaaaagat
     8821 aactttatta gagaatcaaa tagataaaat aattagtgat aaaaatttga atgatatgag
     8881 ggtatctatt gttaacaaaa atattttgtg tagacaaaaa agtgactgct ttggaggttt
     8941 taccacaatt actatttcag aaaatggaga tttatatcct tgtttattta ctttaggaga
     9001 aagaaaattt atcataggta atattttaaa tggtaatggc atcaatcagg ataagctcaa
     9061 gtctattttt aaagaaattg ctaatgagcc aaatgaatgt aataattgca atgcaaatga
     9121 tttatgcttt ggtaaccagt gtaaattctt aaactatatg gtaacaggaa aatataatgt
     9181 tgctccaagt atcaattgta gactaacagg tgtagaatta aatacttata aaaagttatg
     9241 taaaggtagt gaataaaatg aattatctaa ataagtatta tgtaaaggac ttggcgaata
     9301 tatctataga caaaaaagaa gtttatatta gtgctaaatt taaacctgaa tggtttaaat
     9361 tccctaaaat ttgtttagat atattggatt attcatttga taagcaaata acagttggag
     9421 aattgcttga attgtttaat gatgaaaatg acaagatata ctttaaaaaa attcttagta
     9481 acttagataa aattggtatt ttaatgagca aaccaatttc aaatgtgtat gtgtctagat
     9541 taaataaagt aacatttgca attacaaata aatgtaattt aaagtgctca ttttgcagtc
     9601 aaaattcaaa aatagataat caagaagaat tatcctttga aaaaataaaa gttattatag
     9661 ataaagcaat gatttttaat ccgttaaaag tagtattaac cggcggagaa cctatgataa
     9721 ggaatgattt ttttgatatt gtagactata taaaatccaa ttatcaagta gatatacaac
     9781 tttgcacaaa tggaactctt atagataatt ttaatattaa taaatttaaa aatagtatta
     9841 atgcagtaga tttgagttta gatggatttg atgagcctag ttgttcaagt tatagaggga
     9901 agggtgtata tgaaaaggta gtgaatagta tagacctttt aaaaataata ggaatacata
     9961 atattagtgc atctatggta gtaggaaatc ataatattga tatagtagat aaatttgtga
    10021 gcttttgtaa tagtaagtct ataattccta taaaaagaga atttatgagt attggaagag
    10081 gaaaagaagc aataaaatat ttaaaagatg agaaggattc aatgttttat ccagattaca
    10141 acaaaataga caaaaaaata ggtgctcgta cttgtagtac tggtattaca caactatata
    10201 tagatcataa aggcgacatt tatccttgtt ctttgttaga ttcagatgaa tataaaatat
    10261 gttcagctat ggatttagat aataatatcg tagataaagt atataaaaaa gaaatggatg
    10321 tatataaaaa attagctgag cttagtccct taaattatca taaatgtaag ggatgtaaat
    10381 ataaattgtt ttgtaataat tgtatagcta atatgaaaat tatgattgaa agtgataaag
    10441 aatttgaaca taattgtcag aaactaaaaa atatttacga gaagtgttta aattagtaag
    10501 cacttaaggt gtagtattat aggagggcaa atttgtgaat aatgttaaag ttgcagtgct
    10561 agatacaggt atagatatgg aagacagcga aataaaaaaa ttttgtaaat ttgataagaa
    10621 tttacaaata caaagtatta atgaatgtat agatgattta aatgggcatg ggacttttgt
    10681 atctaaaact attatcaata tatgtaaaaa tattgatata tatcctatta aaatatttaa
    10741 taaatttggt aggacaaatt cattaaatgc tattttggta cttagaaaaa ttttaaactc
    10801 agaaatagat ataataaata tttcagcatc tacatttgat tatagttatg aaaatgaatt
    10861 tcgtgatatt tgtaataaat taaaatcaaa aaataaaata gttatatgtt caaagcacaa
    10921 ttcaagagaa gttaataata gtataccgac aatatttgat tcagtaatag gtgtagaggg
    10981 gcatagaaat atatatgaaa atagtcagta tgtatataag aaaaataata aaattcaaat
    11041 gttagctaat aataaagagt cttttgtaaa atttaaaggt agtataattc cttttggaaa
    11101 aaatagtaag gcgtgtgcag tagctacagg aattattgca aatatattaa aatacaatcc
    11161 tggtatttat tttaatcaac ttgaagaaat attattatct aacagtcttg atgaaaataa
    11221 attgtgtact agtaatttat tagatataaa atcctataat aatttaaatc aagaacatgt
    11281 attatatata aataaaggga ttttagaaat tataaataat aatttcgcag tagaaaaagt
    11341 tgattttcaa atgattcagg agtatggaat catgaatact attactaata taggcaa
//
LOCUS       DAEMHZ010000131        11367 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000131 DAEMHZ010000000
VERSION     DAEMHZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.131"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            275..778
                     /locus_tag="KPK72_003089"
     CDS             275..778
                     /locus_tag="KPK72_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG2490283.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            825..1784
                     /gene="moaA"
                     /locus_tag="KPK72_003090"
     CDS             825..1784
                     /gene="moaA"
                     /locus_tag="KPK72_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG2490284.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1788..2258
                     /gene="moaC"
                     /locus_tag="KPK72_003091"
     CDS             1788..2258
                     /gene="moaC"
                     /locus_tag="KPK72_003091"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG2490285.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2294..2725
                     /locus_tag="KPK72_003092"
     CDS             2294..2725
                     /locus_tag="KPK72_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG2490286.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
     misc_binding    3156..3367
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3530..4819
                     /locus_tag="KPK72_003093"
     CDS             3530..4819
                     /locus_tag="KPK72_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2490287.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            4873..5952
                     /locus_tag="KPK72_003094"
     CDS             4873..5952
                     /locus_tag="KPK72_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG2490288.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            5973..6338
                     /locus_tag="KPK72_003095"
                     /pseudo
     CDS             5973..6338
                     /locus_tag="KPK72_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            6978..8096
                     /locus_tag="KPK72_003096"
     CDS             6978..8096
                     /locus_tag="KPK72_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2490289.1"
                     /translation="MIAVKKAYKFRLYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYFSQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTISSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            8223..9392
                     /locus_tag="KPK72_003097"
     CDS             8223..9392
                     /locus_tag="KPK72_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG2490290.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(9555..10097)
                     /locus_tag="KPK72_003098"
     CDS             complement(9555..10097)
                     /locus_tag="KPK72_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG2490291.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVKLGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(10245..10529)
                     /locus_tag="KPK72_003099"
     CDS             complement(10245..10529)
                     /locus_tag="KPK72_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2490292.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(10592..11356)
                     /locus_tag="KPK72_003100"
     CDS             complement(10592..11356)
                     /locus_tag="KPK72_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2490293.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         4300 a         1275 c         2008 g         3784 t
ORIGIN      
        1 ggacaatgtt ataataatta tagaaattaa aagtacttac attcattatg tatgtgacat
       61 gtataattag ttttatattt aatacagttt tgaagtaata tcttcgtttt gagaacgttg
      121 gaataataaa aaatgtatta ttataggtga tataaataaa aaataaatat aatatgttat
      181 attgctgata gaaaagagaa taaaagaaaa taggttttat taaaagagat ttttatttat
      241 aggacttaat caatgtatag aggggtgaga acatatgttt aatgttgcaa ttataacact
      301 tagtgataaa ggatatgaag gaaaaagaga agatataaca ggaaaaaagt taactgaatt
      361 tgttgaaaat acaggagctt ataaagttac tgaatatgta cttataaaag atgataagga
      421 aatgctaaag gaaaatatta taagactttg taatagtaat aaaatagatt taattcttac
      481 aaatggaggt actggttttt caaaaagaga tataacccca gaagcaacta aggaagtgtt
      541 ggaaaaagaa ataccaggtc tgagcgaata tatgagaatg aaatccacag aaattactaa
      601 aaaggccata ctaagtaggg gtgtaagtgg aataagaaat aattctataa taataaattt
      661 acctggaagt cctaaaggtg cagttgaaaa tctaagtttt ataattgatg ttttagacca
      721 tggcatagaa gttttaagag gagaagctac agaatgtgcc actaaaaaaa gtgaatagta
      781 tataatctag atagataaaa aatagacaaa gaggtagata aagtatgatt gataagtatg
      841 gaagaaaaat agactacctt cgtatttcac ttacagataa gtgtaattta aggtgtgttt
      901 attgtatgcc accagatgta aaatttgata aaaattatat aaatgaaaat ctaagctttg
      961 aagactacaa gtttattata aaggctatgg ctgaacaagg tataacaaaa attagattta
     1021 ctggaggaga acctctttta tatcctaaat taaatgagct tataaaattt acaaaagaag
     1081 aatgtggaat aaataacata ggaatgacca ctaatgctat tgggctttct gatagaatct
     1141 ttgagcttga aaaatgtggc ttaaataaag ttaatataag tttagattct ttgaaggaat
     1201 ataagtataa atccgtaaca agaggaggaa acttaaaaga tgtcttaaaa tcgattgaat
     1261 cttgtttaaa cttaggtata aaagtaaagt taaattgcgt tgctataagt gggtttaatg
     1321 atgatgagct taaagatttt atgtttttaa cagttaaatc accaattgat gtaagattta
     1381 tagagcttat gccattaggt gaggctaata gagtttataa gaagggttat tttaatgtaa
     1441 aagaaatgat tgaaaaaata tatggtttat ataaagtcaa aaatgatgaa aaaagtacag
     1501 cagaatatta tatgtttaaa ggagctaaag gaagaatagg aattataact ccattaagct
     1561 gttcattttg taacacttgt aatcgtatta gacttacttc tagtggtgca ataaaattat
     1621 gtctacattc aaaagaagaa atagatataa aaccattttt acataaacca ttgttattta
     1681 gagaatctat gaaagatata ataaaacaaa aaccagaaaa acatcattta atagaaaata
     1741 aatgtagtga tacaaatagg cgtatgtatg aaattggagg ataagtaatg ttaacacata
     1801 taaatgaaaa agggtatgcc aagatggtgg acataagtga aaaagatgat actaaaagaa
     1861 cggccgtagc tacagctaca attactctta gtaaggaagc attagaaaaa gtaaaaaatg
     1921 gtcagataaa aaaaggagac gtattgtctg tagctcaagt tgcaggtata atgggagcta
     1981 aaaatacatc ttcaaacata ccaatgtgcc atcagattaa attagttgga tgtgatttag
     2041 aatttgatgt agatgattct atgtctgcta ttaagatata cgcaacctgt aaatctcttg
     2101 ggaaaactgg agtagaaatg gaagctttaa atgcatgttc tttagcagca cttacaattt
     2161 atgatatgtg taaagcagtt gataaaaaaa tggttataag taatatccat cttgtggaga
     2221 agactggtgg aaaatcagga gattttaagt tcgagtaaat taataatata ttgggttaaa
     2281 atttggagga aatatggcga aggtagtgtc aataaatata agtgagaaaa agggtgtaat
     2341 taaagttcct gtacaatgtg cagagctaaa aataaatcat gggatagttg gagatgcaca
     2401 tgcaggaaac tggcatcgtc agataagctt acttggaaaa gaaagcatag ataaaatgaa
     2461 aatgaaaggc tttgaccaat taaaatatgg agattttgct gaaaatgtaa ctacagaagg
     2521 gatagaagtt ttttccctgc ctgttggtac aaagttaaga atagggcaat gtgaagttga
     2581 agtaactcaa atagggaaaa aatgtcacaa tggatgtgaa attaagaaat tgacaggtga
     2641 ctgtgtgatg ccacgagaag gaatttttgt aaaggttcta aaagaaggaa ttataaatgt
     2701 gaatgattca atagatgtaa tctaatgaag tcactattaa aatataatta taagtaaata
     2761 ctattaagat ttaaatatat attttaagag agaattaatt ttaataaaat attttagtta
     2821 taaattggaa tattggttat ataaagctat gaatgtaaaa tcatagcttt ttgtttttga
     2881 attttaaaat ataatatttt atttatatat attaaattat attttatata aaatcaattg
     2941 tttaaatata tattatttat actgtaaatt tatagataat aaagtataaa aaaaatatat
     3001 tttgatgaaa tttaaaaaaa tacattatat aaatatagta atagtatgaa aatttacata
     3061 taaaactaaa aaatgcaagt aattgacttt ttaagttgac aaaatattgc atatatatta
     3121 tgatataaat cataaattaa ctacaaatca atattatata ttcgtagaag aagaaagtag
     3181 cataaaggaa ttttacagag agttagattt ggctgagaac taatattgga atttatgtga
     3241 aaaggacttt ggagaatact attgtaatta tgttagaata gtggtttaat tcgccttaag
     3301 aaaatgagtg agtaaaatta atttttacta ataagagtgg taacacggat ttatattcgt
     3361 ctcttggatt tttccaagag gcgtttttta ttataaactt atttcagaaa atttaaaact
     3421 atatattaaa tgaaaattat aaaaatatga tagttaataa atttataaat tagaaaattg
     3481 taaaatatgt taagatcagt agttttacga tttaaatgga gggatgaaaa tggaagaaga
     3541 aaaaattaac tatgctctgg aacagattcc agagaatcaa aaacgtggtt gggtagctat
     3601 gttttcagtt ttagtagcaa taggtgtgga tttatcatct gttattttag gtgctgagtt
     3661 ggcacagagt atgccaatga aacaagcaat attgtcagta attgttggtt catttttttc
     3721 tgcaattttg tatactacat gttctttggt tggttcatct acaagtttat ctacatctat
     3781 gataacaaag tatgtatttg gagaagctgg agcaaaaata ttttctctag ttataggggt
     3841 ttctctatta ggttggtttg gagttcaagt aggtttcttt gctcaaaatg cacagataat
     3901 aataaaagat atatttaatt tagatgtcag tatgcagatt ttaagcctaa taggtggtct
     3961 cttgatgatg agtactgcaa tttatggata taaggcaatg gagaagttga gtgtgtattc
     4021 agtaccattt ttattagttc ttatgatgct aactatattc ttagctttta gagcaaatgg
     4081 aatttcagtt gatgataata tgaaatctac tatgacattt gcaggtggag tttctctgtc
     4141 aatgagtata ataatagttg gtgcaattgt atctcctgat ataagtcgtt gggctaaaag
     4201 tagaagagac tgtgctcttt caagcttttt gggaatacaa tttggaaatg cttttatgat
     4261 aatagtttcg atagtattag taaaatgtat gggaacttca gatattatga gaatttttat
     4321 tacattagga attgctatac caggtataat agtacttaca ttagcacaat ggactacaaa
     4381 tacaagtaat gtatattcag catcattaag tatagctctt gtacttaaga aggctcctga
     4441 gaaagtatta actatagtcc ttggtattat agcaacatta ttggctgtat ttggaatata
     4501 tgaaggtttt ataggatttt taaatttact tggaattgta attgcacctg ttggtggtgt
     4561 atatacagca gaatattaca tagtaaagca agaacttaaa ggctttgata aaggagtatt
     4621 atataaacca atagttaaac gttcaatagt gtcttggata attggtatat taataactta
     4681 cctttcaaca tatggattta taactcttac tactatagca cctttagatg gttttatagc
     4741 agggtttgta gtacaatcta ttattggaaa agtgctttgt tcaacaaaaa ataagcaaga
     4801 aatagacaaa gcagtataaa gaaatttgtt tataaagtat aaaataaatt agaaatatga
     4861 ggagaaagta acatgagatt tttaacagaa gaaagtgtag ataaaatagc agttggtgca
     4921 gcagtgcttg gaactggagg tggaggtgac ccatatgtag gtaaattggt tgcaaaacaa
     4981 gccattaaaa agtatggtcc tgtaaaagta ataagtttag atgaacttga tgatgatgct
     5041 ttggtagttc cagtatctgg tatgggttct cctgtaatta caatagaaaa attactttca
     5101 gaagtagagt taactacccc tttagaaata atggagaaat tattaaatag aaaagtggat
     5161 gttataattc ctatagaaat aggagggata aactcactta tgcctatagc agttgctgct
     5221 aaaaaaggac ttcctatctt agatgcagat tctatgggta gagctttccc tgaagctcaa
     5281 atggtaactt tttatcttga aggatatgag gcatctcctg ccgtaatgtc tgatgaaaaa
     5341 ggtaattctg ccatccttta tcctgtggat ggaatttggt cagaaagatt agctagaact
     5401 ttaactgtag aaatgggagg aagttcttct attagtgact ataatttaag tggagctcaa
     5461 gttaaaaaag ctgctatagg taatactctt acaatagctg aaactatagg agggttttta
     5521 cttgaaaata aatctgattc aaaaggtgct gttgagaata tactaaaaga attgtgtgga
     5581 tacaagttat ttgaaggaaa agtaattgat attaagagag agttgaaagg tggatttact
     5641 agaggacatg ccttttttgc tgggattaat gagtatgatg gagagtattc tatattattc
     5701 caaaatgaga atttaattgc caaaaaaggt gatacaccac tttgtattac accagattta
     5761 atagcagtat tagatttaga aactggattt ccaataacta cagagagaat aaaatatggc
     5821 tctcgtgtta tggtagtagc ttttccttgt aatgaaaagt ggagaacaga aaaaggaatt
     5881 gagacagttg gacctggata ctttggatat gatgtggaat ataaaacagt agaagaatta
     5941 caaggaaaat aaaagtttag gaggaaattt tgatgtatcg tgtaggaatt gacgttggag
     6001 gaacaaatac agatgcttgt atactggatg gggaattaaa agtaattcat tcagttaagg
     6061 tggcaactac aaaagatgtt gaaactggtg tttacaatgc cttaaaaaaa gttatagatg
     6121 aaagtaagat tgaccattca ttaataaaat atgctatgct tggtacaact cattgtacaa
     6181 atgctattgt agaaagaaaa agacttaata atgtggctat gataagaata ggaaagccag
     6241 ccactactgc cattcttcca tttatagatt ggccagaaga cttaagagaa aaaatagaat
     6301 tagattcaat attggtatct ggtggatatg agtttgatta aaaaatagct acgcacactc
     6361 gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac ttatatgtag
     6421 agataggata gaacctatcc aatactactt gaattgctgg aaacccctaa agctaaccaa
     6481 actaaaacat aaggatgaaa taaacctaag tgtgaaagtt acgaaagtag aaaaaattgg
     6541 ttagatggta taaggttaaa ccctaagtac tttaaaatgg gcaatcagca ggtaaactcc
     6601 gaataggaga aacttcaacg actattcctc ttgagggaag tacactacaa gctattggta
     6661 gtggaagtgg gtagacctta acggataatg ccgaaggata agatatagtc tgtgcttacg
     6721 tgaaagtgta agaagttcat aagagaactg cataggtagt agcgagctta tgtgaacgac
     6781 aacctcaaaa atgataaaag ttaagactta cggttcttaa atatgttatt taaaattaaa
     6841 tataaaacct tctaaataat tgttttaaat aattacattt gttaatatta tgataggtgg
     6901 ttaataaatt gttcaccaca gcattatata atatattaaa atcattaaaa agtgtatcaa
     6961 aaaacaatta aagtgaggtg atagcagtga aaaaagctta taagtttaga ctgtatccaa
     7021 ataaaaagca acaagagtta attaacaaaa cttttggatg ttgtagattt gtatataata
     7081 agtatcttgc taaaagaata gatgtttata aaaacaataa agaaactttt acttataaac
     7141 agtgtagttc tgatttgacc aatttaaaaa aagaattaaa atggcttaaa gaacctgaca
     7201 aattttcact acaaaacgcc ttaaaagatt tagataatgc atataaaaaa ttctttaaag
     7261 aaaaagcagg atttcctaaa tttaaatcaa agaaaattaa tagattttca tataaaacta
     7321 actttacaaa tggaaacatt atgtacttta gtcaacatat aaaactacct aaacttggta
     7381 tggttaaggt aagggataaa caagtaccta aagggagaat acttaatgct actatatcta
     7441 aagaaccaag tggtagatat tatgtatcat tatgctgtac tgatgtagat attgaagcat
     7501 ttgaaaatac taataatcat ataggtttag atttgggaat taaagaattt tgtatctcaa
     7561 gttgtggaga atttatagaa aatcctaagt atcttaagaa gtcattaaac aaacttgcta
     7621 aattacaaag agaattatcg agaaaaacaa tcagtagttt aaatagaaat aaagcaagat
     7681 taaaagttgc aagacttcaa gaacatatag ctaatcaaag aaatgatttt ctacaaaaac
     7741 tatctactaa attaataaaa gagaacgata ttatttgcat agaagattta caagtaaaga
     7801 atatgattag aaatcgtaaa ctatctcgct taatttctga tgtatcatgg tcagagttta
     7861 ttcgtcaatt agaatacaag gctaattggc atggtagaca aattgtaaag gtaggtaaat
     7921 tctttgcaag ttcacaaata tgtaataaat gtgggtataa aaacgaggaa gttaagaact
     7981 taaatataag agaatggatt tgtcctagtt gtaatgaaac tcacgataga gatataaatg
     8041 caagtataaa tatattaaaa gaaggattaa gactaataac aattcaaaat aaataacgta
     8101 tgtaagaacc gtaggaacta cggggatagc ctgtggagaa ttagtaagac atattttagt
     8161 atgcaaaatt ctgtgaagca ggaaccctgt gactttagtc atgggaggtt cagggtagaa
     8221 agataaatga actttgtaaa gatgaagttg tagagttttg caatagaata aaaggtaaag
     8281 tggaaagtgt tgcaataagt ggagtatttg ctccagtaaa taaacaacaa gaagaagaag
     8341 ttgcaaaatg ggttagagaa attttagggg atattcctat atctttatca aatgaaatag
     8401 gtaatatagg tatattagaa agagaaaatg gagctatttt aaattctgca ttgtctcaag
     8461 taggtaaagc tgtatcttta ggatttgaaa aagcacttaa agacttaggt atagatgctc
     8521 aaatatattt cagtcaaaat gatggaacat taatgaattt agagtataca atgaaatacc
     8581 caatttttac aattggatgt ggaataacaa attctataag aggagcttcc ttcttatcaa
     8641 aagtaaaaaa tgcaatagta ttagatgttg gtggaactac aagcgacttg ggtgtactat
     8701 ataatggatt ccctagagaa tcatctattc cagttacaat tggaggagta catacaaact
     8761 ttagaatgcc agatgtttta actattggtc ttgctggtgg tacatgtgta aaaggcgaaa
     8821 aagataatat aaagataggt cctgaaagtg tgggatatag aataactgaa gatgcaataa
     8881 tatttggtgg agacactcta actttatcag atgttgttac aaaattagat atggcgtttg
     8941 aagatagaaa aagcaatgtt gaaaacttag ataaagagta ttgtaatgag gtatataaga
     9001 aggttatttt aaaattagaa gatgctgtag accaaatgaa gactagtcaa gatgatgttg
     9061 aattggtttt aactggtgga ggaagtataa ttgttcctga aaaactaaaa ggcataagca
     9121 atgttataag acctcctcat tatactgctg caaatgctat tggtgcagct ttaggacaaa
     9181 taagtggaga tgtagaaaaa gtatacagtt tagataaaat gtcgagagaa gatgccatag
     9241 aagatgcaaa aaaagaggct gtaaataaag ctctaaaagc tggtgctaaa gaagatacta
     9301 tagaagtatt aaatatagaa gatgttccac ttgcatacct tcctggtaat gcacttctta
     9361 taaaagtaaa agttgttggt gatttaattt aaagaataaa tataagtatt tccagaatta
     9421 tataaatatt tctggactca tttttaaact tattttatca ataaaaacag cataactttt
     9481 gactatattc ttataagttt ccgaacagcc gatatatacc tattttttga ggtttatcgg
     9541 ctgtttgtta tcttttatcg cattccaact atttttctta ccgtatttgc tgtacgaatt
     9601 gtaatcttat cacttacatt tgaactggca gtctttgacc accaatttgt ctttttataa
     9661 tctttacgac taaaagacca aaagatagca tttttataat gatatacttt ttcatactct
     9721 tctttaggat ttccaacctc atcataaaac tcgtcataag ataagtcagg aaacataaaa
     9781 ataagattat catatatttc tttgttgtta tctccccacc aattaggagc attatttagt
     9841 atatctttta actcttcttg tgaaataata aaaactggta tttccaattc aaactgtttt
     9901 tttatcatta atcttattgt atttgagata accttgctgt catctacatc acttaaaaag
     9961 acaacattac cactattaag gtatgtagaa acttctgaat aaccaagttt tacaaagctt
    10021 gcttttaatt cagacataat tatcttgttc ttaccactta tattgatacc tcttaataat
    10081 gcgatataac ttttcatata ataatttcac ctctaaacat tcacattttt ctatgtagta
    10141 caaactggaa tttgcatctt ttataattct ttcaaaactt cttcatatat aattttttta
    10201 atataaaaaa atcggtgctt aatttgtacc gatttttgtt tttcttagta aagaactcgt
    10261 ttttttgctt ctgctatagc tctagcagca ctttttgctt gcattctttt ttcttcttct
    10321 tttttaaatt catttattgt ttcatttaga ttcttatcat gttcattttt ttctttatct
    10381 tcgttttctc tgtatactaa tatataatct attattgatt cgtttattat ctgggatata
    10441 cttttatttt cattaattcc tattctctta acttccttaa gtagagtctc atcaatatga
    10501 accgttgttt gctttgtatt ttttgccacc ttaatcacct cttaaaaaaa attctaacat
    10561 taaaattttt tttagtcaat aaattttaat attaaaattc tttaaatgtt ttatttgatt
    10621 ttctaaaatt ttctattgta tttatcatct tattttttat attttcacct agtacagaag
    10681 actttagtct aggattttcg atatggtcat aaattggttc taaaaattcg acttcgacag
    10741 gaacagagtt aattttacca ataggttggt atccttcata agtatctttt gaatttttta
    10801 ttactagtgg aacaataggg cattttgctt tatatgctat ctttaaagct ccacttctaa
    10861 actccgaaac caatgaattt ggttctttaa tccatgttaa atctccttct ggaaacactg
    10921 ccatactttg acctgtaagt atattttgag aggcttgggc tatactcttt ataccttcac
    10981 gattgttttt tctgtttaca tatacacatc ttagtaattt tgcccattct ttaaatatag
    11041 gtatatttct ccaaactggt tcatctgcaa ttacacatcc aattggtctt tctacacttg
    11101 ctgttagtat gaagctgtca agcatactag aatgatttac aacaaataga agtggttctt
    11161 ttggcagtgt ttccttacca attatattaa gctctatgtt cactatatct aaagattttt
    11221 tagcttgttt ttgcataaat tcaaattttt cctttaagct aaatttatca ggattctttt
    11281 taattttaat aagcctaggt atagaagatg taaaacctat aagttgatat gtagcaaatt
    11341 ttaagtaatt attcataaaa ttatctc
//
LOCUS       DAEMHZ010000132        11248 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000132 DAEMHZ010000000
VERSION     DAEMHZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.132"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            264..1010
                     /locus_tag="KPK72_003101"
     CDS             264..1010
                     /locus_tag="KPK72_003101"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG2490294.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            1040..1975
                     /gene="rsmH"
                     /locus_tag="KPK72_003102"
     CDS             1040..1975
                     /gene="rsmH"
                     /locus_tag="KPK72_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG2490295.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            2456..2803
                     /locus_tag="KPK72_003103"
     CDS             2456..2803
                     /locus_tag="KPK72_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490296.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            2815..4794
                     /locus_tag="KPK72_003104"
     CDS             2815..4794
                     /locus_tag="KPK72_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG2490297.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            4961..6334
                     /locus_tag="KPK72_003105"
     CDS             4961..6334
                     /locus_tag="KPK72_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG2490298.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            6375..7343
                     /locus_tag="KPK72_003106"
     CDS             6375..7343
                     /locus_tag="KPK72_003106"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG2490299.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            7362..8717
                     /locus_tag="KPK72_003107"
     CDS             7362..8717
                     /locus_tag="KPK72_003107"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG2490300.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            8769..9899
                     /gene="spoVE"
                     /locus_tag="KPK72_003108"
     CDS             8769..9899
                     /gene="spoVE"
                     /locus_tag="KPK72_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG2490301.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            9917..11143
                     /gene="murG"
                     /locus_tag="KPK72_003109"
     CDS             9917..11143
                     /gene="murG"
                     /locus_tag="KPK72_003109"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2490302.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
BASE COUNT         4451 a         1202 c         2179 g         3416 t
ORIGIN      
        1 tagaagaggt tgatgtatat atgcatcaat ctttttgatt aaaaaatatc tatgtacact
       61 tgtaacttta gtcatgagag attcagttta aaaaatttta tagtaattag atataatata
      121 gtgtagggag ttgtagaata aattttaata ttttttgtag aattaacatt aatgtgttat
      181 cataatttag tataattaca attaattgta attatagcat attgctttgg aaatattttc
      241 aattaataca aagaggtgac gttatggata gagtagcatt tacactgttt ggcatagaca
      301 tcatgtggta tggaatactt atggcatgtg gaatgatttt aggaacttta atagcaataa
      361 aagaagcaaa aagagtggga attaaagaag atgatgtatt aaatatagct ataatagcaa
      421 ttccagttgg actaatctgt gcaagaatct attatgttgt atttaattgg agttactatg
      481 ctcaaaatat gtctcaaatt tttaatttta gaggtggtgg ccttgctata catggagggt
      541 taattggtgg aatattagct ggttatatat acactaagat taaaaatatt aacttcttaa
      601 aaatggctga tacagttata cttggaatgc ctttagcaca agctattgga agatggggaa
      661 attttattaa tggagaagca catggaggtg ctaccaattt accttgggga ataatggttg
      721 atggagtaaa ggtacatcca acttttttat atgagtctat atgggatttt ggaatattta
      781 tagttttatt actatttaga aaaaataaaa aatatgaagg tcaagttata gttacttata
      841 taactttata ttcaatagga agatttttta tagagggatt aagaacagat agtttaatgt
      901 taggaccact tagaatggca caagttataa gtctaattgg agttattggt ggcataattg
      961 cacatgttta tttatcaaag aaaaataaaa ataatattag tgaagaatag tttaaaaagt
     1021 gaatttaagg gaggtaacta tggaatttca tcatgtatca gttcttttaa atgagtgtat
     1081 agagaattta aatataaagc cagatggcgt gtatgttgac tgtactatgg gaggagcagg
     1141 acattctaaa gaaatagtaa aaaaattgtc agataaggga ttatttatag ggtttgacca
     1201 agataaaaat gctataagca cagcaaaaga gagattgagt gaatatgaaa gtagagtgaa
     1261 gtttgttcac agcaattttg aaaatataaa agaggaatta gaaaaaatag gagtttacaa
     1321 aattgatggt gtattagctg atttaggagt ttcatctcat caacttgatg aagctgatag
     1381 aggattttct tatatgcaag atgcaccact tgatatgaga atggatgtta ggtgtgagtt
     1441 ttctgcatat gatgttgtaa atacttatac agaagatgaa ttaactaaga taataaaaga
     1501 ttatggagaa gataactggg ctaagcgtat agctaaattt atagttgaag aaagagcaaa
     1561 caaaccaata gaaactacag gagaattagt agatgtaatt aaaaaagcta tccctaagaa
     1621 agctagaata gatggacctc acccagctaa aagaactttt caggcaatac gaatagaagt
     1681 aaataatgaa cttggagtaa ttacaaagat gataaatgat gcttcttcta taatgaatga
     1741 aggtggaaga atatgtataa taacattcca ttcattagaa gatagaatag tgaaaaatgc
     1801 atttaagcat ttagcatcag attgtatatg tccacagcat ttacctatat gccaatgtga
     1861 taaagaatct gaggtgaaaa taataactag aaaacctata ttacctagtg aggaagaaat
     1921 agaagtaaat ccacgttcaa gaagtgctaa gctaagagta gcagaaaaaa tataagtagt
     1981 tataaggcta aagaataatc ttatatataa aattattata attaattaaa aagtattata
     2041 tattttgttg cactggtaaa gcaattaaaa aacaattaac actataagaa atgttctttg
     2101 ttattgagat atataatgaa agtttagata taaaaatata gatatagatg gtattaagta
     2161 atataatgga gaacttagga atcagagagt agatagttag ttacatatta tatttatgga
     2221 agaaattatc taaaaatact agaagtgata aaaataaata gaaattaata tctaacctat
     2281 agattcatat aaaagtataa gtgaatttat aggtttttgt tgtgtataga aaaaaataca
     2341 attagattgt gtaatagtgt attaatttag cgatgaaatt ttagaggaag aaatatgatt
     2401 aataacaaat atagtataga ggaaaattta aactatatac tatcggggtg agaatttgaa
     2461 aaaaaattat aaaatacata atctaaatga atataaaaat aaaaaaaaga ataattataa
     2521 aaagagaaat aagaaaaaaa taggagctaa atgcatttta ctgattgcaa ctttttcagt
     2581 aataatattg aatctatgtg gttattcaaa aatctcggaa atgaagtacg atatctatta
     2641 tttacaaaaa gatttaagaa aaaaagagat agtattagag gaactaaagg caaaagaaac
     2701 tgaaaaaaca tcgataaaag aaattgagct aaaggctaag gaattgctca atatggacta
     2761 tccaaaagat ggacaaataa agtatataga tgtggataac tagggggaat agatttgaga
     2821 aaagtaaaga ggataagtaa gaaaaggctg gtactcgttc ttattctagc atgtgcattg
     2881 tttttttgcc ttgttattag gactgggtat ctacaactta tgaagggaaa ttggttgagt
     2941 acaaaagcac tagaacaaca aacaagagat ataccaatag aacccaaaag aggaactata
     3001 tatgatagaa atatgaaaga gttagcagta agtgtaacta agtatactgt ttggtgtaaa
     3061 cctgtggaag tagaagataa aaaagaagca gccgaaaagg tagctgagat tttggatgaa
     3121 gattataaag atatttatgc tttaataagc aaaaaaaata tggctcttgt aaaagtaaaa
     3181 agatggatag atgatgataa ggcaagtcaa ataagagatg ctaaattaag tggaatatgg
     3241 gtggcagaag ataatcaaag atattaccca tatgggaatt ttgcaccata tgtgttagga
     3301 catacttctt cagatgcaac aggtatatct ggtgtagaaa tgcaatatga taaaaagtta
     3361 aaaggaaagc ctggaaaact tatagtaagt actgatgctt caggaagaga aataccacaa
     3421 ggaatggaaa aatattatga gcctgtacaa ggtaatggtc ttgtgttgtc tatagatgaa
     3481 gtaattcaac actatactga aaaagcagta cagaaagctt atgagttaaa taatgcaaaa
     3541 aaagttactg caatagctat gaaccctaaa acaggtgata tacttgcttt agcatcaaaa
     3601 ccagattatg accctaatga ctcaagaacg ccaatatatc catattatca agaagaactt
     3661 gaaaaatata atgataaaga taaaataaaa ggctattatc aaatgtggag aaatccagca
     3721 gtaagtgata catatgaacc aggttctaca tttaagctta ttacatcttc cagtgctctt
     3781 gaagaaggag taattaaaga tggagaaaaa ttcacatgta caggtagtgt aactgttgga
     3841 ggaagaaaga ttaaatgttg gagacattac agacctcatg gaacacaaga atttaaacaa
     3901 gcagtacaaa actcttgcaa cccagtattt gttgagttag gaagcagatt aggtgttgga
     3961 aaaatgtatg attatataga atcatttggt cttatggata agacaggtat agatttacca
     4021 ggagaagcta agggaatact gtataacgaa aaaaatgtag gaccagttga attggctaca
     4081 atatcatttg gtcaatctat atcagtaact ccaatacaac ttataacagc tatatcatca
     4141 atagctaatg gtggagattt aatgcaacca agagtggtaa aatcttatac tgacaataaa
     4201 ggaaatataa ctgaaacagt aaaaccaaaa aaagtaagaa gtgttatatc taaagaaaca
     4261 tctaaaaaaa tgttagaaat tgcagaatca gttgtaactg aaggtggagg aaagatagcg
     4321 tatataccag gttatagact tggtggtaaa acaggtacag ctcaaaaagt tatagatggt
     4381 aagtatgctc caggaaagta tatatgttca tttgttggaa tagcaccttg tgatgacccg
     4441 caaatagtag ttcttgctat tgttgacgaa ccaacaggag taagtgcatt tggtagtact
     4501 acagcaggac caatagtgaa agaaataatg aatgactctt taaaatattt gggagttaaa
     4561 ccagtatata aagaagaaga aaaagctgag tatgaaaaga agcaagttaa agtgccagat
     4621 gtaagaaatc taaaaatagg agatgctgta aaggcattgg aggatgcgaa gttaaaacca
     4681 gacttagatg cagatataga acttccagaa gatacaaagg taaaggatat attcccaaaa
     4741 cctggagtaa aagttaatga agactcaagt ataaccctat attttgaaaa ctaaaaagta
     4801 acagataatt aacatatacc cataaacatc ttcaaaaaaa cagaaatata gtatataatt
     4861 ataaagttaa gagttatcgt aggcaatgta tatataacgg tatacattgc cttttgttgc
     4921 taagagaata tagctaaggt tttaaaggag gagtttaata atggagtgct taacaataaa
     4981 agagttagta cttgcaacta atgggaagtt aatatatgga gattataatg actgtgtaag
     5041 tgatatagtt atagatagca gggaggcaaa tgcccaaaat gcatttgtag ccatagttgg
     5101 agaaaattta gatgggcata catttatgaa gtctgcatat gatagtggat gtaaaacttt
     5161 tataaaaaat gaaagtaatg gaataaaatt agaaagttcg gatataaatt taatagaggt
     5221 aaaagataca tctttggctc taggagatat ttcaaaatac tataaagaaa aatttgatat
     5281 tccatttata ggtgttacag gtagtgtggg aaaaactact actagagata tgatttactc
     5341 tgccatatct gcaaagttaa atatactaaa aaatgaaaaa aacttaaata atcattttgg
     5401 agttccattg actttattta atctaaataa agaacatgag tgtgctgtaa tagagatggg
     5461 tatgtctgga ttcaatgaaa taaaatattt agtggatata gtaaatccta aaattgcagt
     5521 catatcgaat ataggattat cacatgtaga aaaactaggt tcacaagagg gtattttgaa
     5581 agcaaagatg gagattactt caaactttga tgaaacaaat actttaattg taaatggaga
     5641 tgataagttt ttatctacat taaaagaaaa agaacatgta tataagttaa aaacatttgg
     5701 ctttaataaa aacaatgatg tttattgtga aagttatact atggaagagg atagtttaac
     5761 atttatatgt gtaataaatg ggaaaaaaga ggaaatattt ataccaacaa taggagaaca
     5821 taatatatat aatgcaatgt ctgctatatt agttggtttg tgtctaaata tttctttaga
     5881 ttatataaaa aaaggtttaa aaaattttaa agggacaaaa atgagattag acataataaa
     5941 aaatgaaaaa ttaacaataa ttaatgattc atataatgct agtccagatt ctatggatgc
     6001 tgctttaaag atacttggaa gatataaagg tagaagggta gcgatacttg gagatatact
     6061 agaaatgggt gaaatttcag agtatggtca tagattagtt ggaaaatcat ctatgaataa
     6121 tactgatatt ataataacta taggtgaaaa ttcagtgttt attggagaag aagcaaaaca
     6181 attaggattc aattcagcca atatatatca ctttgagaat agagaagatg tgtttaacaa
     6241 gttaaatgaa ttagtaaaaa caggagacac tattttagta aaaggctcaa gaggcatgag
     6301 attagaaaaa atagtcgaat atctaaataa ataagcttaa actaatgagt taattgacaa
     6361 gggagaggag ttttatgatg ttaggaataa cagagcttac gtacaccgca ttgattgcgt
     6421 ttttaatagt aattataata ggaccaatat ttataccaat gcttagaaaa tttaaatttg
     6481 gtcagacagt tagagatgat ggaccccaaa cacatctagc taaaaacgga acacctacta
     6541 tgggtggaat tataatgatt gttgcaattc taataactgg acttacaaga gtaaaagtta
     6601 gtcatgatat ggcagtaggt cttatatgta ttgcaggttt tggttttatt ggctttttag
     6661 atgattttat aaaaataaag ctaaaaagat cacttggact aaaagcatat caaaaaataa
     6721 tccttcaagt tgcattatct ttttatgttg ctttttatca gtatacatca tcatcaagtg
     6781 cttcacaact tatgatacca tttacagatt ttgtgataaa tgttggaata ttatatatac
     6841 caataatgat gtttataatc gtagcaatag taaatgctgt aaacttgact gatggattag
     6901 atggattagc atcaggagta acattaatag tttctgtatt ctttatgcta tttgcaagtt
     6961 ctattgctgg aaatacagaa gtcgcagttt tagcagcagc aactgttgga gcatgtttag
     7021 gtttcttagg atttaattca tatccggcta gagtgtttat gggcgacaca ggttcaatgg
     7081 cattaggagg tgcagttgta gcattttcag ttctaactaa ctcagtgctt ataataccta
     7141 taattggagg aatttatttt gcggaggctc tatcagtttt gattcaagtt ggatatttta
     7201 aggcaacaag aaagagattc tttaagatgg ctccaataca ccaccacttt gaacagtgtg
     7261 gttggccaga aacaagagtt gtatttattt tttggattat aacagttgtt ttagcttgga
     7321 taagtataat agcagtattt tagggctagg gggaatgaaa aatggactta aaaggaaaaa
     7381 aagttttatt agtaggactt gcaaaaactg gtatatctac aattaaacat ttagataaat
     7441 tgggtgcaag tataatcgta aatgatataa aagatgaaaa taaattgaga aatatacttg
     7501 atgaattgaa atctataaat gacataaaat acatattagg acatcaccca gaagatgtag
     7561 atgatataga tatggttgta gtgtctccag gtgtaccact agacctacca ttcatattga
     7621 aattaaaaaa ttcaggcaag tatataattg gtgaggtaga attagctttt aaactatcga
     7681 ataatccaat ttttataggg attacaggta caaatggaaa gacaactaca actagtttgg
     7741 taggagaaat tttttcaaga gcaaaacgag atacatatgt agttggtaat ataggaaatc
     7801 ctgtaataga tactatagaa acttcaagtg aagagtctgt gttagtaact gaactaagca
     7861 gttttcagct tgagagtata gatgaattta gacctaaagt aagcgcaata ttaaatatta
     7921 cagaggacca tttaaataga caccatacta tggaaaaata catagaagca aaggcaaata
     7981 tatttatgaa ccaaactgtt gaggatttct gtgtattaaa ctatgatgat gagatagtaa
     8041 aatctttagc tgataaatgc aatgccaaag taatttactt ttcaagaact aaaaaagtaa
     8101 atggaggagt atatttagaa aataatgaca taataataga tatagatgat aaaatcaaat
     8161 ttctaaataa agatgatgta agtttacctg gaggtcataa cttagaaaat tgtatggcag
     8221 ctatagcaat agcatatgtc tgcaaaattg atttagaagt tataaaggat gtacttatga
     8281 catttaaagg agtggaacac agacaagagt ttgtaagaaa tttagacaat gtaatatatg
     8341 taaatgattc aaagggaact aatccagatt ccactataaa agctatacag tcttatgata
     8401 gacctataat acttatagca ggggggatgg ataagggaag taattttgat gaacttcttg
     8461 aaactgcaaa gtcatatgtt aaatcattgg tcttattagg agaaactgca tcaaatatag
     8521 aaaattgtgc taaaaataaa ggatttaatg acatacatat agtaaaagat atggaagaag
     8581 cagttaagac ttcttatgaa atttcaaaaa gtggagatat agtgcttctt tcaccagctt
     8641 gtgcaagttg ggatatgtat gaaagttttg aagtaagagg aaaagacttc aaagataatg
     8701 taaacaattt aaaataatct cttacctcta aacttactta aatggaagaa tggaggtaga
     8761 ttagttggat gcctaaagaa aatttgaaaa aacaaattga cataaggatg aagactgaat
     8821 ttgatggagt agtgttttac actactatgc ttttagtatt tgtaggaata gttatggtgt
     8881 ttagtgcaag ttttatacaa tcttcattta aacacaatga tgcgtactat tttctaaaaa
     8941 agaatgtaat atatgctgta cttggattta tagttatgat tattacatca agaatagatt
     9001 atagtttttg gaagaaaaat gctacagcaa ttggagctat agcagtagta ttattattgt
     9061 tagttttgac ccctttaggg atagaagcaa acggagcaaa aagatggttg ggaataggag
     9121 ctttaacatt tcaacctgca gaaatcgcaa agtttgctac aataatatta actgcaaaat
     9181 taattgaaaa aaattatgat aaaataaaat ctttgacaaa aggtgttgta ccacttctcg
     9241 tagttccagg gatatttttt gcgttgataa tacttcagcc aaatttgtca acagcaggta
     9301 ctgtaattct agttactttt gtaatgatat ttgttgctgg aatggatatg aaaattgtat
     9361 ttgctatgat agggagtggg gctgctttat ttgctgcatt agtaattgct gaaccctata
     9421 gacttagtcg tgttacatca tttttagacc cttttcaaga ccctcttggt aaggggtatc
     9481 aggtaataca aggtctatat gcactaggtt caggtggatt gtttggtcta ggcttaggaa
     9541 agagtaaaca aaagtatttt tatataccag agccacaaaa tgactttata tttgctataa
     9601 taggagaaga gttaggattg attggttgta taatagttat tatgctattt gtagttttag
     9661 tctacagatg tgtaagaata gctttgaaga cttctaatgt atttgcttgt atggttgtta
     9721 taggtatagg tgcacaaatt ggaatacaag cagctttaaa tatagcagtt gcgacatcat
     9781 caatgccagc tacaggtgta gcactaccat ttataagtta tggaggaact tcactcacca
     9841 tctttatggg agctgtagga atagtgttaa acatctcaaa gcatgtcaaa attaattaac
     9901 aggagatgat gatttgatga aagttttatt atcaggcggt ggaactggag ggcacgtgta
     9961 tccagctata gctattgcaa acaaaataag agatgagcat ccagacgctg agataatttt
    10021 tgttgggacg gaaaagggaa tagagtcaga aatagttcct aagtatggat ttgaattaaa
    10081 gactgtaaca gttcaaggtt ttaagcgaaa aatagatttc gataatgtaa aaagagtttt
    10141 taagcttttt aaaggtttgg agcaatcaag aaagattgtt aagaaattta aaccagatat
    10201 agttatagga actggtggat atgttagtgg acctgtttta tttaatgcat ctatggggaa
    10261 aataccagcc ataatacatg agcaaaattc ttttcctggt gtaactaata agattttatc
    10321 taaaacagtt acaaaggtac ttacaagttt tgaagattcg cataaaagat ttccagaggc
    10381 agcagaagat aaattagttt ttacaggaaa tccagttaga aaagaaattt tattatcaag
    10441 aaaaaatatt gcaagaaaga atctatcaat aagtgacgaa aaaagaatgg tactttgcta
    10501 tggtggtagt ggaggttcaa gaaaaataaa tgatgcaatg agacttgtaa taaagaatat
    10561 ggtaaatgaa gatatagctt ttatatttgc tacaggcaaa agctattatg atgaatttat
    10621 gggaagtatt agcgatataa atcttaaacc ataccaaaaa gttgtgcctt acttagaaga
    10681 tatggcaaat gcacttgcag caagtgattt ggttataggt agtgcaggag caatttcatt
    10741 agctgagata acggctcttg gaaagccatc tataataata cctaaagctt atactgctga
    10801 aaatcatcaa gagtataatg ccaagagtat agaaaaacaa ggggcaggaa ttgctatttt
    10861 agaaaaaaat ctaactccag agagcctaaa tacagccgta tttaaacttt taggagatag
    10921 agaacttttg gttgatatgg caaatgcaag taaaactata ggtaagcctg aagccataga
    10981 ccttatatat gatgagataa tgaaagtata caattcaaca caaaagtcaa cttctaaaaa
    11041 aacaaaaaaa gaaaaagtta ttaaggaagt aaaagaaata aaaaaagaaa cgactccaag
    11101 tatagaaggt caagctaagg taataggaat aaagaaaaga taagtaaata tttaccccta
    11161 atttataata tattagattt tctaaattat tataaattag gggtttattt gtatgtatac
    11221 agatttaaat atataaatag tatgtagt
//
LOCUS       DAEMHZ010000133        11231 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000133 DAEMHZ010000000
VERSION     DAEMHZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.133"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            149..1324
                     /locus_tag="KPK72_003110"
     CDS             149..1324
                     /locus_tag="KPK72_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2490303.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1497..2894)
                     /gene="asnS"
                     /locus_tag="KPK72_003111"
     CDS             complement(1497..2894)
                     /gene="asnS"
                     /locus_tag="KPK72_003111"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG2490304.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(2971..3204)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3373..3567)
                     /locus_tag="KPK72_003112"
     CDS             complement(3373..3567)
                     /locus_tag="KPK72_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG2490305.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3840..5513)
                     /locus_tag="KPK72_003113"
     CDS             complement(3840..5513)
                     /locus_tag="KPK72_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2490306.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5523..8921)
                     /locus_tag="KPK72_003114"
     CDS             complement(5523..8921)
                     /locus_tag="KPK72_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2490307.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDMGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEDLSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9089..10285)
                     /locus_tag="KPK72_003115"
     CDS             complement(9089..10285)
                     /locus_tag="KPK72_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG2490308.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFIGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNPTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10306..11127)
                     /locus_tag="KPK72_003116"
     CDS             complement(10306..11127)
                     /locus_tag="KPK72_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG2490309.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3572 a         2060 c         1308 g         4291 t
ORIGIN      
        1 acattatcta ataaatttta tctatcacaa ttttagattg ttacacacaa tatattttga
       61 tataattaaa tcataaacga agtattccaa atatctaatg atagaaagga atacttattt
      121 ttatgcttta tttttaatgg aggcttatat gaacttagaa aaaataaaaa aattttttgg
      181 atttcctcta gacaaaaaac acaaagacaa tgttgagaaa gaactctgca attatcttta
      241 tcatacgggt aaaatcattt gcataataat agtaatatct caaataatta tgatgatatc
      301 aatctcagct agagaaggag gcccttttgt cacaccaaga cgccaatggt atttttatat
      361 gtatgtaaca ctattttgtg tatcttctgt ctttatgttt gtattttctt acctttggaa
      421 taaaaaaaag gattgctgta cgttttatat aaatttatgg ataatttatg tgactttttt
      481 ctgcctatgg ggttgtgtaa ttactctact tgaccagtta ggtggaaatg acctaagtgt
      541 atttagttat attatgttag cagctgcagc tttctcagta ttaaaatttg gagaatcagt
      601 aatagttttt ggaggatgct ttttgtttct taatttattc ttacctggga ttcaaactat
      661 accaggcaat gagtttagta atattgtcaa cacatttttt ataacatccc ttgccatttt
      721 tgtttctaca atgctatata gaaatcgtgt atttacttac tatgataaac ttttaattag
      781 tcaacaatat gaagaaatat ctcatataaa taatttactt aacgacaaag ttatgacaga
      841 tgaccttact aaaatgaaca accgtagata ccttgaagat attctacaaa aaaaattaat
      901 cagtcatgca aatcaagaac ctataactgg aatgatgata gatatagatt attttaaaca
      961 atttaatgat aattatggac atcaaaaggg agactattgt ctacaaaata ttgccaaagt
     1021 aattttagat tttacaagtt ctgaaaatga acatgctata cgatatggtg gtgaggaatt
     1081 ttttatatgt atttttaact gtaatgaggc agatgctaaa cttaaatcag aatgtttacg
     1141 aaaaattata gagtcctgca attttgagcg tgatgatgtt cctttaaaat gtgtaactgt
     1201 tagtattgga gtttatacaa agaaaattgg aaagacaata acattaaatg agtttataag
     1261 taattgtgat aaggctctat atactgcaaa agaacaggga cgtaattgtg ttgcatttta
     1321 ttaaaaggtt acatactatt acataaacct ataagtatta tatcgcaaaa taaaagtaat
     1381 caaattatat atttatagta caataaaaat aacttgcata tctctaaaat agataaatat
     1441 gcaagtcatt ttagtgttta tacttaaagt tatataagtt ttattatata taattattaa
     1501 aattcagcat ttttaggagt tcttgggaaa ggtataacgt ctcttatgtt agacatacct
     1561 gtcatataca taattattct ttcaaatcca agaccaaatc cagagtgagt agcagtacca
     1621 aactttctaa gttctaaata ccaccaataa tcttcttctc ttaatcccat ttcattaatt
     1681 ctatctaaca atacatcttc tctttcttct ctttgagaac ctcctacgat ttctccaact
     1741 ccaggaacta gtaaatccat tgctctaaca gttttaccat cttcattcat tctcatatag
     1801 aaagctttta tatcttttgg ataatctgta acaaacactg gacaattata tatttgctcc
     1861 gttatatatc tttcatgctc tgtttgaagg tcacatcccc actcaacagg atactcaaat
     1921 tcatgcccag attcaattaa taattccact gctttagtat acgttattct aataaactca
     1981 gagtttacta tcttatttaa tctttctaac aatcctttat ctataaaact attaaaaaat
     2041 tccatttctt caggagcatt ttctaataca tagcttataa catattttat catatcttct
     2101 gcaagttcca tgttatcttc aaggtctgca aaaactattt ctggctctat catccaaaat
     2161 tctgaagcat gtcttcctgt ataagagttt tcagctctaa atgtaggtcc aaaagtataa
     2221 acgtttctaa aagaaagtgc cattatctcg gcatttaatt gaccactaac tgttaaatta
     2281 gcctcttttc caaagaaatc ttgagtatag tctattttgc catcttcagt ttttggaata
     2341 tcatttaaat ccatagttgt tattctaaac atctctcctg caccttcaca gtcactacct
     2401 gttatgattg gagaatgtgc atatacaaag tttctttctt ggaagaattt gtgtattgca
     2461 taggcagcta ttgatcttac tctaaataca gctgaaaaag tattacttct tggtcttaaa
     2521 tgtgctatcg ttcttaaata ttcaagcgta tgtctcttct tttgtagtgg atacgaacta
     2581 tctgattccc cttctacaac tatgcttttt gcatgtattt cgactggttg tttagcccct
     2641 tctgtagata ctaactctcc ctctacaagt atagatgagc ttattgctaa ttttcctacc
     2701 tctttaaaat tttctacttt ctcatcaaat actatctgca tatttttaaa gaagcttcca
     2761 tcatttagct ctataaatcc aaaattttta gatactctag atgttcttat ccatccagct
     2821 acttttacag tcttccctat gtactgttct ttttctctgt acaattcttt tactgttata
     2881 aattcctttt gcattttaaa tcctcctagt ataattgtta ttaatttctt ttaaatataa
     2941 tttcaataaa aaaagccctt catccctata taaagggacg aaagactatt actctcgcgg
     3001 taccacccta gttgtcataa tgacctctta gttaagcaat gcttaaagcc tataacggtg
     3061 ccttccgtct aagtctactt actaaataag ctttcgatta gaaactccaa gatgttcttc
     3121 aatataaacc tcgtattgag ctttcaccat cctcaactcg ctagaactac attttatatc
     3181 tactcttctc ttcacagtat ttatctttta ttatttctgc taattaacaa aattatacca
     3241 atttataaat ttttagtcaa taaatttata taattgatac tgattgttat ttaaaatata
     3301 ataattctac cttacataat gataataaat acttatcttc attacttctt tattacttca
     3361 atttccaaaa ttttataatt tacttccatt gtcaacccag tcttttgctt cttcgacacc
     3421 cttaattgaa ggtattgaaa ctttactatg aggttttaat ttttcaacat cagcccatgc
     3481 tgctgtcgac tcatttccaa ctaaaagtga acttttaagt cttttattat ctttaccttt
     3541 taatgctctt gcctttttac tttccaaaac tatcacctct attatttatt ttgtaatagt
     3601 tttagcaatt ttacaaataa aattcataag tctaaattat aattattttt aaattaacat
     3661 catatctttt caatacttaa tttataccta tttatataca aaatatttac tttgtgtatt
     3721 caaaatataa aactgttttt taattttcat atttattttt tatgctattt ttctactcta
     3781 tctaaagtat ttgatacaat taaactaata atcaattata attttacata ggttcttatc
     3841 tatagagtat ttgctatctg ttgaatcgta ttttttaaat ttaaaatccc atatccctgc
     3901 gatacatttg gatatgtata tatctcaagt tttgtagcac ccaatattaa atatgttttt
     3961 aaaacttgtg taaacaatga taatctcggt acattatcct gtttttctaa gtattccatc
     4021 aataaggcta aaacacctgt gactatagaa ctgctcactc ctgttcctgt tcctgtatta
     4081 tatgttccat ttttatatgt ggatattata tctacacctg aagctactat atctggctta
     4141 attcctcttc ctctaattgg tcctttcgaa gaaccaatcc acatactatc agtcttattg
     4201 ttaaatgtac caactgtaat tacatcatca ctagcagcat acatagttat agtagctact
     4261 gaatcagggt ctaaaaacct tgtgtctgga gctatcaaat ttttatttgg caagtatatg
     4321 tcatattctc catttattat aagttctgaa gtcaatctta aaatccatac tccaggtttt
     4381 atatctctta atcttatctc caaattttct tttccagagg taatatatgg ataaataaat
     4441 ctcattgcat aagtagtatt ttctaagtta aattttcctc tatatatata aaagtctgga
     4501 gaatacctta tatcatgact aacttcacct gaaggagata ttatctgtgc tcccacttta
     4561 tctggaccat tcgtatttag tgttatatca agagcatagt catctccatc ttgtattatt
     4621 acatcttgaa cttctcccac actgctaaat ctgccagaat aatgaatatc tgtattccct
     4681 tgatttcctg caccactcac tactaccaca ccagcactac tcaatatatt aaaagtatct
     4741 agtattgaag tagttgctac agcacttgac ttaactccaa tagtcaaatt tattatcagt
     4801 ggtttatttt ctgttcttgc tatgttagta acatacgtta tagctgctaa aaaatctgaa
     4861 acactgtaat ttatccttcc agcataataa gtatctgtat atgattttaa tttaactact
     4921 attaagtctg attctgtagt aatacctctg tactgagaat ttatccttcc atttccaact
     4981 aatataccag aaactaaagt tccagtacct atattatctt gactcaaact cccatcattt
     5041 ctatttatgg ctatatttaa ctcacttctt gtaaattcac ttccaaaaat aaaaccttct
     5101 ggaggtggat ttgtatttgc ttcttggtcc cataaatata aaactttact agttccatca
     5161 tcatttataa aatcaggatg caaataatct atcccagaat ctataactgc taataaaatt
     5221 cctctccctg ttatatcatt atatggattt tcataaatat aatcagtttc agctgctgtt
     5281 gtaatggttt ctccattatt tacattatta gttatttcaa ttagactgct cattggttca
     5341 gattcttccc accaagcaac ttgtattata ttatttaaaa tagtttcatc aaaatctaaa
     5401 ggtacatata ttactgcaag ctgactattt aaaaccatat acctatttat tccattttct
     5461 tgtagggcag attctatatc accttgataa attatacaat aagatttttc cataaatccc
     5521 tcctatctta aaacatcaaa cattcctcta acatccaaga gcccatatcc agaatttgta
     5581 tttggatata ctgtgtttga atcttttcta gcaccagctt gcataaaagt ttttattttt
     5641 tgcacatatg cttggtttgg atatctaccg tcaacaaaag tatattgaaa atacattgca
     5701 gcagcaccag ctgcatgtgc acttgctgct gctgtaccag ttatagttgc atacgtgttc
     5761 ccaggataag cagctattat attaacacct ggtgctacta tatctggttt taacctgtct
     5821 tcaatagttg gccctcttga tgaactttgc cataaactac cattaatggt attgtaggct
     5881 cctatggtta ttaaatcatc ttgaatcgca ggataattta tagtgtaaaa agggtctacc
     5941 tctctaaatc tagttccact ttttaaaagt tctctatttg gaaggtataa gttatatctt
     6001 cctgttataa tataaactcc tactaatcga atcttccata cacctctttt tgcattcttt
     6061 aaagtaacat tagtaaattg ctgtccagaa aaagttgttg ggtatatata tgttatagaa
     6121 tattcagtcc cctctaaatc aaataaacct gttactttat tataatttga aattccaaca
     6181 cttttacttt cttcaccagt gggagatact attattacat cagctttatc tggtctattt
     6241 aaccaaagtt ctaatgataa gtcttcctcg tcttcattta actctaattc aacttcaaca
     6301 gaacctccaa catatggtat tattccagat gtatgtgtct gtgtattacc ctcatttcct
     6361 gctcctgccg ttatacataa ccctctagta aagaacgctt tttcactatt actcctattg
     6421 gttaaacctg ctaggctact ggtacctaaa gacatattta taactaatgg tcttccaagt
     6481 tcaaaagctt ttttatatgc atactgagat gcagcaaaca acattgcact attataaaaa
     6541 tcatctatct tgccgagttt tattattatt agctcagaat cttctgctat acctgcatat
     6601 tcactattta catttccaag ccctgcacat atcccactca acatagttcc ttgaccaact
     6661 tcatcctgag atagactagg gtcattttct gcgatagctc gatttatatc ttctcgtgta
     6721 tattctgtcc caatataaaa accatcagga ggagttcctt cttttgtttg gtcccataaa
     6781 tatactattt ttgaagtgcc atctggataa ataaaatctg ggtgtagata gtctattcct
     6841 gtatctgcaa ttgatattag agtccctctt cctgttatat taatattagg attattttta
     6901 aagaaattta cgcccatttc ttctgttgca actacacctc caaaagtacc tcgattaatt
     6961 tctccaagta atgtcatctt tgtagtcgat acagttctta tgattgaagg aagttgcaaa
     7021 actcgttgta tactactata gtctgtatta tttatagata gtacacctaa tgaatcactt
     7081 atcttaaaaa acctttgact catacctaac ccagctagtt cttcttcaaa ttcaggagta
     7141 tggactatat ctattcctga tagtattcct gattgtctac ttgcattttt acttatataa
     7201 atttcattct ctaagtcatt atgtatttgc atattataac catctataaa cttataacta
     7261 ttttcctcat ctttaaatga cttattaata cttctatatc ccatatcctc tgtttctaaa
     7321 tcttgattta tattagatag agtgtacaaa tctatatctg ataaatttaa aaaaccaaat
     7381 cctgatgaat tatttggata agattgatta cttaatctcc ttgctccctt tagaagtaat
     7441 gcccttaatt tctgtgaata caaaaataaa tcatttccat ttacaattcc ccattccata
     7501 aacaatgaac aaacccctgt aacatgaggt gtagccatgc tagttccagt taatgctcca
     7561 cttgtacctc ctggtaaaaa agatactata tcttcacctg gagctaataa atcaggttta
     7621 aaaacaccta attgtgtatc tccttcacca gaaaatatag atactatatc tgttcttgaa
     7681 ttaaaactac ctactgttat aactctactt gctgttccag gaactgttac agtaagctct
     7741 tgagtaggaa tcagaaatct agtatttcta tttagacctt cagaagttgg caaatatata
     7801 tttacattac cagttactat atctattggt tcaaatacaa ttttccataa cccaggagtt
     7861 atctgtgtat ttgaacttaa ttgtagagta actcttcttg tgagagaata cggtgctata
     7921 gggtaaaaat aaccagttat tctagtttca cccaaggtat tccttatctc acctgaagtt
     7981 agagagattg cttgtgtttg attatttgat gggtttacca aatgtacact aaaatcatca
     8041 acaaaatctg gccatatatt tatgtttaat atacgctctc cttccccaac tataaactca
     8101 acttcttctg taatattatt ttgaagccta atccttttat gacctccttt atctgcatta
     8161 tttccagctg ctacaactat attgtttttc caaaataaac acatatcatc tatatattgt
     8221 tcaaataaag atgttcctct atgtgaacct tcattacttc cataacttat atttaatgta
     8281 acaggcattc ttaattctaa tgccctatct aaaataaatt taattgccct cataaactct
     8341 gtacttctcg aaaaaatatc ggtctgtata tttccaactc taaccactat tattctggca
     8401 tcacttgcaa tagttgcaca aatacctgca acatgtgtcc catgtaagct agttgttgaa
     8461 ataggtatat acatacttcc atctattgca ttatttatat cttcattagt atataatgtt
     8521 ccttctctaa aaccttctgg tggatttcct tgaatagatt ggtcccaata atataatatc
     8581 ttagaccttc catcactatc tctaaacaca ggtagagtat aatctattcc cgaatcaatt
     8641 ataccaatta tagttccttt tccagttaaa ccagttctat ttttaaatcc tgttattcct
     8701 gtacttgaaa aactttgcac atcctgagtc tgtaatataa aaggcttttc tataaattca
     8761 atttctggat aagttaaaag tatatttaca tcttcttcat tacttgatgt tattattgca
     8821 tatgatgaat taagtatctc tactgataca cctaattctt cttctaacct caatatatct
     8881 ccattgtatt ttacaattaa ttcataattt attataatca cctcgacttc taaaattatt
     8941 attaatgtat atgttttgtg tgagtctaaa atgtattaga ctatataatt ttttaagttt
     9001 cagttataca atttataata gatatattaa aaaaaataaa taatgatggt acaattttga
     9061 ataaatcata ccatcattta tatttacttt atcttgaaac cattaaattt ttcaatgctt
     9121 tctcaagacc ttttacagtc aatggaaaca tattaaattc actttgtatc attctttgag
     9181 tttgatacca ataattaatt gaatcccatc catctctaag ttctggattc atccatacaa
     9241 ttttactgta ctttctctta aaccttttaa gccactctat accaggtgtg taattatatg
     9301 gaccacgata gttaccacca atatgaagca actcagaagg cgacatagaa gcatctccaa
     9361 caattataac tttatagttt ttatctatat tttttaatat ccattcagta ttaatagatt
     9421 tacttaacca acattcaggt gttgtaaata atctatcata aatacagtta tggaaataat
     9481 atatctttac atctttaaaa tgatttgatt tacttacaga ctgaaataat gtattacata
     9541 aagtgctata ccctctcatt gaaccacctg aatccataag caataaaagc ttcactgtat
     9601 tttttcttgg cttctcaaaa aataacttta aatatcctgc attattacaa gtctcttcaa
     9661 tagtcttatc caaatcaagc tctgtctttg gagcatctat tctagtagaa aattgacgaa
     9721 gtctctttag tgcaacttga aattgacgca tatttaaaac ttcatcatct ctaaaatctc
     9781 tgtattttct ttctcctgca acttcaagaa cagctctacc ataagtagga tttccaccta
     9841 tacgtatacc agtttgccct tttcctgaat gtcctaactc tgaagtgcct cctgtgccta
     9901 tccaataatt accaccatta tgaggagagt cttgttcttt tattcgttcc tccaatttag
     9961 ctcttaattt atcaagattt atatttggat tttgttctaa tctttcaaac tcttctaaat
    10021 ctatatctgg ttttttaagc caattcataa tctcttctgg aatttcttct tggtgttcta
    10081 tacctataaa atactccaaa aacactgcat caaacttatc aaaatcactt tcacttttta
    10141 ttaaaatcat cctgcaaaga tagtaaaaat ttgaaaagtt tgaatagcac agtcctttat
    10201 ccaatgcttc aattaaagtc atccattcat ttaaagatac atttaatcct cttgacctca
    10261 ataaataaaa aaattcaata aacataatta tccagctcct attaactata agtgtctttg
    10321 catagattca atatcctcat tattcttaag tagtattcct gcaaaaggaa cttctttttc
    10381 tattttttca ataggcattc cactcaaagt taatgcttgt atccagtcta ttaactctga
    10441 tgtactaggc tttttttgta tgtcctttaa acttctaatc caataaaaag tagtcattac
    10501 ttgttccaat aggtgttctt ctactttatc aaaatgtact ttaactattt cttccatcat
    10561 gtctctatca ggaaattcta tataatgaaa tatgcatctt cttaaaaaag catctggaag
    10621 ttctttttcc gcatttgaag ttataataac tattggtctt tgtttggctc ttacagtttc
    10681 ttttgtctca tttatgtaaa actccatttt atccaattcc caaagcaagt catttggaaa
    10741 ttctaaatcc gccttatcta tttcatctat taaaagaatt acttgttgat ttgagctaaa
    10801 ggcctctcct agtttaccat attttatgta cttgctaata tcatctacac cttctcctcc
    10861 aaattggcta tcataaagtc tttgaacagt atcatataca taaagccctt cttgtgcttt
    10921 tgtagttgat tttatattcc aaattactaa atcttttttt aattcatttg aaattgcttg
    10981 tgctagcata gttttacctg ttccaggttc gcccttaatc aaaagtggct tttcaagagc
    11041 aatagcaaca ttaactgatg ccatcaattc tggtgatact acataatcac tacttcctct
    11101 aaagttagat atctcttttt ttatcataaa tcctactcct tttaaacagt attttctaaa
    11161 tgtatataga ctttattact atttttgata taaaaataat aatacaatac atttgtatag
    11221 tattattcta a
//
LOCUS       DAEMHZ010000134        10948 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000134 DAEMHZ010000000
VERSION     DAEMHZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.134"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1322)
                     /locus_tag="KPK72_003117"
     CDS             complement(<1..1322)
                     /locus_tag="KPK72_003117"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG2490310.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKAL"
     gene            complement(1471..3276)
                     /gene="lepA"
                     /locus_tag="KPK72_003118"
     CDS             complement(1471..3276)
                     /gene="lepA"
                     /locus_tag="KPK72_003118"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG2490311.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3558..3809)
                     /locus_tag="KPK72_003119"
     CDS             complement(3558..3809)
                     /locus_tag="KPK72_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490312.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3840..4859)
                     /locus_tag="KPK72_003120"
     CDS             complement(3840..4859)
                     /locus_tag="KPK72_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG2490313.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4877..5830)
                     /locus_tag="KPK72_003121"
     CDS             complement(4877..5830)
                     /locus_tag="KPK72_003121"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG2490314.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6172..6948)
                     /locus_tag="KPK72_003122"
     CDS             complement(6172..6948)
                     /locus_tag="KPK72_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2490315.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6982..7503)
                     /locus_tag="KPK72_003123"
     CDS             complement(6982..7503)
                     /locus_tag="KPK72_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490316.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            8102..8368
                     /gene="rpsT"
                     /locus_tag="KPK72_003124"
     CDS             8102..8368
                     /gene="rpsT"
                     /locus_tag="KPK72_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG2490317.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(8555..9598)
                     /gene="holA"
                     /locus_tag="KPK72_003125"
     CDS             complement(8555..9598)
                     /gene="holA"
                     /locus_tag="KPK72_003125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG2490318.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(9552..10853)
                     /locus_tag="KPK72_003126"
     CDS             complement(9552..10853)
                     /locus_tag="KPK72_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG2490319.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         3567 a         1839 c         1170 g         4372 t
ORIGIN      
        1 tttaatgctt tttcttttag tatttctttt tcatttaact ctggattatt tatgactatt
       61 tcaagcatct tatttaaaat atatcctata tctttacctg gttttatacc tatagatatc
      121 aaatcttttc cattaatagc taaatcttta agattaaagc actccttttg atttattata
      181 tgtttaaatt ctttttcaca ctcaataaga tttaatatct tctgttttgc atactttgga
      241 tttttggcaa gtgtatcagc ccaattaact tttattaaat cttcaaataa tccaacatct
      301 tcaagtttat ttaataactt tttaattatc ttccttttag gttctattct ataatcatga
      361 tattgtatta atattaaaac tttattaata gtataattat catacttaag tcttttcaaa
      421 atcttctttg caatctttac agattctcta ctatgagtat aaaaatggtc aacaccattt
      481 ttatcagttg ttttagttac ttttttacct aaatcatgaa acaacattgt aagttttaaa
      541 tacagttcat cttcaattac ttctgtagct attaaagtat gtttttgtac atcatagatg
      601 tgatatggat tgtgttgagt aacatcttct aattcacata tttcaggaat tataaatttc
      661 aacaaacctg ttgattttaa taagtttaat ttagatgaat cagatactaa tattttatta
      721 aactctgtct ggattctttc aatagaaaca ttttttatca aatctgcttt tttatgaata
      781 ctttgcttag taccttcagc tattttaaaa ttgagttgtg ctgaaaatct aactgctcta
      841 agcattctaa gtgcatcttc ttcaaatcta tctactggat ttccaacaca acgtatatac
      901 ttattttgta tatcttcata accattaaat gggtcgacta atcctgtctt tgaattgtat
      961 gcaattgaat tgatagtaaa atctcttctt gacaagtcct taactatgtt atttgtaaat
     1021 tctattgaat ctggatgtct accatcacta taatttccat caattctata agtcgtcact
     1081 tcaaatggtt catgttcaat cataactgtt acagtcccat gttgtatacc agttggaatt
     1141 gttttttcaa ataattctac taccacttct ggtctagcac tagttgttat atcccaatcg
     1201 tttggaactc tttctaataa acaatctcta acacatcctc caactatata agcttcataa
     1261 ccattctttt ctaattcttt aattatatag tctacttttt taggaatttc tatattaatc
     1321 ataaatatca cctttttatt cttctttaat attcttactt atattctcta tgagtttact
     1381 taaaatatac cataaaaaag ctagactaat ttagaaataa ttctaaaatc actctagctt
     1441 ttaaatatct ttatctaaat ttataaggat ttactcatct aatttaagca cagacataaa
     1501 tgctttttga ggaacttcaa cagaaccaat ttgtctcata cgcttcttac cttctttttg
     1561 tttttcaaga agtttcttct tacgagatat atctcctcca taacatttag caagaacatc
     1621 ttttcttaga gcgcttattg tttctcttgc aataacctta ttaccaacag cagcttgtat
     1681 tggtatagca aactgatgtc ttggaatttc tccttttaat ttctcacaca ttgcctttcc
     1741 tctagggaaa gcacgtgtct catggacaat aaaactaagt gcatccactt gctctttatt
     1801 gattaatata tcaagtttta caagtgtaga tgctacataa cctttaacct cataatcaag
     1861 agaaccataa cctctagttc ttgattttaa agcatcaaag aaatcgtaaa caacttcatt
     1921 gagaggaagg tcatagtgaa gcattactct tctctcatct atatattcca tatttaacat
     1981 atttccacgt ctttcctggc aaagctccat aacaacaccc acatagtctt taggaactat
     2041 tatatctcct tttactatag gctcttctat catctttatc tcagatggtt ctggtaagtt
     2101 tgcaggattt tgtatcatta caacctctcc atccattttt gtaactctat atataacaga
     2161 tggagctgta gtaattatat caaggttaaa ttctctctca agtctttctt gcattatctc
     2221 catatgtaaa agacctaaga aaccacatct aaaaccaaat cctagtgctg ctgaagtctc
     2281 agcttcatat tcaagtgcag catcatttac ttgaagcttt tcaagagcat ctctaacatt
     2341 ctcatacttt tctccttctc ctggatatat accacaatat accattggag tagctttttt
     2401 gtatcctggt aaaggttctt ctgtaggatt atttgaatct gttattgtat caccaacacg
     2461 acaacttctt atatctttta tactagcagc aatataacca acatcacctg ctgataattc
     2521 actaagttca gtttgacctg gagccataac accaacttca gttacctcaa actttttatt
     2581 ggtattcatc atttttattg tcataccctt tttaacagtt ccttcaaata ctctaacata
     2641 agctacaaca cctttgtaag catcatagta agaatcgaat attaatgcct ttaatggagc
     2701 ttcattatct cctttaggtg gaggaacatt ttttacaata tcttctaaaa catcttctat
     2761 gtttaaacca gttttagctg aaataagagg agcttcactt gaatcaagcc ctattaaatc
     2821 ttctatttca ttttttactt cctctggtct tgcacttggt aaatctattt tatttattat
     2881 tggtaatatt tctaaatctt ggtctatagc aagataaaca ttggctaaag tttgtgcctc
     2941 aacaccttga gctgcatcaa ctactaatag tgctccttca catgcagcta aacttcttga
     3001 tacctcatag ttaaagtcta catgtccagg tgtatctata agatttaaat aatattcatt
     3061 tccatccttc gctttataca ttaatcttat gttttgaagc tttatagtga ttcctctttc
     3121 tctttcaagg tccatattgt caagtaattg acttttcatg tctctttcac taacaagccc
     3181 agtttgttgt attaatctat cagctaaggt agactttcca tggtctatat gtgcaattat
     3241 actaaaattt ctagttctac tttgtttatt gtccacctta aaattccctc ctcttgatta
     3301 ttcttatagt tattatttta cattatctaa aaactcttta tataccaaat ttaaaatttt
     3361 tgaatataaa ctaattggca ctaaacaaaa ttatccacat tatatgtgga ttatcaagtc
     3421 taacactaaa tattatataa tatcgtaaac ttaaagtaaa tcctataaaa tacagttaat
     3481 gaatttttat tttatacatt aaaaatatat aactaaaata aggcttattt taatcaataa
     3541 accttattca cttattacta cttattaaat ttttttatga tttcatgtat atcaaaattt
     3601 ggtatgtcaa aatcaaattt tgttatatca aatttaatac ttggaaaatc atattgtttc
     3661 attcctagca tactttttgc attactgtct actataaata cacaaactga tgtgcctata
     3721 atcatagtaa tcaaaaataa gattctcata atatacttgt attttcttag tttactttta
     3781 ctttctatat ttttttctaa tcggctcatt tagcatgctc ctaattataa cttaaagctc
     3841 taattttttt gtttaaaata ttcatccaaa atctcggcta aatactttgt agtagcctta
     3901 gattcttcta cacttgttgc gttattccct atttctacta acatatgatt cttaactgca
     3961 aactgattga atctagcata gtctttttct actattggag atactagacc aggatacttt
     4021 ttctcagcaa aagcagttat atcttctgct aaagctctaa gttgttttct atttgtattt
     4081 ttacctccta caacaaagaa gaactttgat actctctctc catttatcat agttgtatat
     4141 tttaaatggt ctttttcctt agtggctgga ttagttaaat ccctagcatc tctatgtaag
     4201 tctatcgcta tatccactga gttatattta ggtaatatac tttgtattgt ttttaagcta
     4261 cttgcataag agttattata agatggatag tcatggtatt tagtagtatg gactacaccc
     4321 catcctttgc tatctaaagc actagttagt gcacttccta cttccattac tgaatttttc
     4381 ttatctcttg aatgataatt tccatcaggt gaatttgaat aagtctcaca tccatgagta
     4441 tgatatatca aaattcttgg attttctttg gttactctta aatcatcctt ataatctgaa
     4501 ctagatgttg tgttagtttc agttgtgtct gaattttttg actcaatatc tggaacattt
     4561 tctttaccaa cataaagttt tatatattct ttttcactct cttttttatt atcgacttta
     4621 cttgcatctt tagatttagt gtttccttct ttactttctt ccttattttc cttacttgtt
     4681 tctttatttt tattattctt tttattttct gtatcatttc cttcgacttt agcttctgga
     4741 taagatgaat ttactaaaaa ctttaaaaaa tcatcttgat tcaaagctaa tgattttcct
     4801 ggtaaaatgc atactaatat aaaagtcaag gttactactt taatgcattt tttaaacata
     4861 attgctcctc cttattttat accaatctac ctggatgtaa agacctattt aagccttcac
     4921 ttattattat agatagattt tcgattgtat catctatatc ctttggtgtt actattaagt
     4981 tcttgtcata tgggtctaac gaatctttta ttagatgata tttttcttct tctttaagtt
     5041 ttttcaacat ttcataaaaa ctttctgttt cttctgattg agctattaaa ttgtctattg
     5101 ccatatctaa gacatctatt gtaagagttg ctgcatcaac caccgttgga acgcctattg
     5161 caataacatc cacgcctaaa gtttctttag ttagagattt tctagtattg ccaacacctc
     5221 caccaggtga tattcctgct gttgatatct gtatagtaga gtttactctc tccatttttc
     5281 ttgaagccaa tgcatctatt gctactactc tatctggctt aaccttctca accaatgatt
     5341 ttactatttc actagtttct attccagtaa cacccataac tcctgggctt aaagctgata
     5401 cctcagtgaa atcatcatca tagtctttat tataattttt aaaaagatgt ctagttacaa
     5461 gtgtttttga tacactcttt ggtcctaacg catcagaagt aatatttcta tttccaagac
     5521 caattaccag ggttttttta tttttgtctt ggccaaatat atctactaac tcctctttta
     5581 aataacttat catttcttct cttgactcat catcatcaaa cttcataaga ttactttcca
     5641 gagttatata ctttcctatg tctttattca ttatctctgc gccttgcctg tctattactt
     5701 cgactttagt tacaatgcaa ttttctaatt ctttagtttc tatttttaca ccaggaatac
     5761 tacttccttc ttgagacttt tcacacattt cactagcttc taaagctaaa tctgttctta
     5821 cactaatcat ataaacctcc agtataaatt aaatgctaat tataattgtt aacaaaattt
     5881 agtattttat aacaagaaat tgtaaattta tttaaaacaa gtaaaattaa ggctttcaat
     5941 gtaagaaaat tgggtttagt cagtgatttg tcagtaaacc ttcttatcta tcatttattt
     6001 acaaacagaa aaaggtagca actatttata taggcgctac ctttaatcta tttataattt
     6061 tcctattaag ctttcactaa tatattaact aaaataattt tttatattta tacctgattt
     6121 atttactata ttaaaaaaca gcaataatta tacaataatc acctaacacc tttattcctt
     6181 ttggaaaaat ggttctgcaa tttccaacca ttcatttgga agataaagtg ataaataccc
     6241 atgattatag ctcttagctt cataaatctc acaagagggc acataagact gaaacatttt
     6301 tgcagatttt ttaacacatt tcatttcttt ttctccatac cagtacatta cttgtgctgt
     6361 ggttttttta acattctctt taagtgtata gttcatcatg taagtgcgat acatattata
     6421 gaatgtttct tttcttacat gtggcatatc tgtcatataa tatgctttta tttcatctgg
     6481 atattgcata ttttcaggta acaattttgg catcatagca atttgaaagc gacaagcctt
     6541 ctcactaaac aaaagtcctc caaaaaatcg aataccagca atacaaaacc ttgccataag
     6601 aggtcttggg tagcaaatac taccatcaat aattgctttt ttagtaatat taggttttcg
     6661 agataaaagt tccatgacaa tttgaccacc aagtgatacc ccacacaaag cgaaaagatg
     6721 tccactgcaa ttttcatcta tatatgatat caacttatct gctgtatcct ctgtagaaat
     6781 gtatgttgtt tcatattctt ctccatgacc atccaatgta ggaagtatca catgatattt
     6841 gttagacaaa acttctgctt gtcgcaaata gttccaccat gcattccctc ccccatgaat
     6901 caacataata tgtggtctgt ttttatttcc aaactcatga aatttcatat ataacctcct
     6961 tttataaaaa tgggttgcat gttattttgt tttatcatta tcagctacag gtggagaaga
     7021 tataattgaa agttttctaa gttttagatt tgatttaggt ttttgttcta tatatggttt
     7081 caataaatca gctatacttt taagaagatt ttcatattca ttatcactca tatatagtaa
     7141 tgctgaagag aaaaatacat tatccttttt atggtcttgc tcttttctgc taaagtattc
     7201 attcatttcc tgcatcagac ctacataaaa tgctgtagaa agtgcaacga aggtctctcc
     7261 ttccatagat gttgtatatt cctttagtgc ataaaccttt tcaatagtac cacgcacacg
     7321 attttcttta attacttcaa tcatattgtt ctcttctaaa atttttacat ggtgataaac
     7381 tgttgcacga ggaatatcat ttaaatactt tacaatatca gatgtacgaa cactgccatg
     7441 aagtcgaata tattgtaaaa tacgaagcct tgcaggattt aaaattagtt ttgctatgtc
     7501 catcttatca gccctttctt tagtctataa aaatattata gtctattttt ttagactatt
     7561 caagtctatg atataaatag taattacaac tataataaat gctacaaatg ctattctata
     7621 taaacactga cattaattac atataacctt ctaacatatt agttatattg tttgatgcat
     7681 ctatcataca tactaatgaa aaaaattatc ttgattattg gaaaaattat cttaattaag
     7741 catataaatt tatattttta taatttttca ttaaatttac aaaaatgagt aatttcttgt
     7801 attatcttat aattattgga aatactatca aaatgtatgt taatagtttg tattatctca
     7861 aaatatttgt tacaaaaata aaatattaat ttatatatgt caaaatattt aaaacacaaa
     7921 atataccaat ttaaaaacaa gatattgcaa attatttata cacaatttac agtctaaaaa
     7981 atcaaaataa aaaacattat taaaatatat aaataataat ccttgatttt tagatttatt
     8041 tctgttaaaa tctaatatgt tgacgatgaa tatgataaaa aaatacaggg aggtgaacac
     8101 ggtggcaaat ataaaatcag ctaagaaaag aataagtgtt atcgaaaaga aaactgcttt
     8161 aaatagagtt agaaaatctc aaataaaaac tgcaataaga agattcgaag atgctgttgc
     8221 agctggaaac agagaggatg ctgttgctaa gttccaatac gctcaaaaaa gaatatacca
     8281 agtagcttct aaaggaacta tacataagaa tgctgcagct agaaaagtag ctaaattagc
     8341 tcaaaaatta aacggtatga acgcttaatt accagtttat atttcttata tataataacc
     8401 cctatgtagg ggttttttat tttgtaaatt tatacaataa aaaataaata gttagacata
     8461 ttatagttga ttgtatatca aacttaattt agcttacatc tactctgaac tataacatat
     8521 cttgctattt attttttatt aatatataga ttttttaaat tgcttctctt ttacagtatc
     8581 tactaaccaa catttcaact gctaaagtat ctcttataag acctgtcttt attttataat
     8641 cactttctaa tatatagttt aatatttcca caattatatc atcagaaaaa tttttagttt
     8701 gctttaatgc ttttcctaca acaaattgat gcatttttaa tttatcagct ataagttttg
     8761 ttgaatatcc atcaagttgt aactgtctta cttgcataat tattttaaat tgtcttgcta
     8821 tcatagaaaa tatacctaat acagattcgc cttcttgtat catatcattt aatattttca
     8881 ttgcattaga agcattttgt tctccaatat aatcgattaa tttgaatata tcattctcaa
     8941 cttttttttg agataattta tcaattacat cattggttac attattccct ttaccaacaa
     9001 atgaacttat tttatttatt tcattttcta aatcagataa tgtcttttca ctactcttat
     9061 ctctgtatcc ttcttgttca ataaaataca tgatttgaga attatctatt ataacatcat
     9121 ttaaagcaaa agattttttt atccatttaa acaaatccat atcagaaagc ttatcacaat
     9181 caaaaacaat tccattgttc cctattttct taactaatga ttttcttttg tctacatctc
     9241 catatacaac aaaaactata gttgtggtgt ctggaatatt gtctaaatgc tctattaagt
     9301 atttctcatc actatctgta aaattctttt tctttccttt taatagttca aagtctttta
     9361 ttattactat ctttctatca tccataaatg gaagtgtttc tatagagctt ataagctgat
     9421 ttaagactat ttccttccca tctattatat ctaagttaaa atcaagcatt ccctcgttta
     9481 gactatcttt aaatgctttt attgcattct caattaaata atactctctt ccataaaaaa
     9541 gatacatttt ttcaaagttt ttctctttta cattccttat aatatccttg taattcattc
     9601 tcttgctcct ttatctcttt tccttctatg taaaatatgt aaaaaaacac tcctatgtaa
     9661 taaataacaa caatataaaa tttaggattt tctatattaa tgtatgcaaa acttaaattg
     9721 ctgatttttt ctagtaaaaa aaatatgctt tctataaatg tactattaaa aaaagatata
     9781 attttagcta ttactacact tactttaaat acgattaaac ttgcaatact taaatacatt
     9841 attactccca caaatggtac tataattaaa tttcctataa ttgaaagcaa tggtatacct
     9901 ttaaatgtat aataaattat aggtaaagtt aatatatttg atgatattgt caatgatacc
     9961 atacttaatt ttataatact atttatttta ttatagaata ttaatataga aagtgtagct
    10021 aaaaaagaca attgaaaact tacattatat ataacatagg ggttattcat aattaaaatt
    10081 atcccaatta gagacaatgc cgatattcca tctttctttc tatcaataaa tattgataaa
    10141 taaaaaatca taacaaatgc tatagaccta attatggatg gagacgcacc taccattata
    10201 caatataaag ttataaaaat cgacagtatt aataatttat acaatttatt tatacctctt
    10261 aatagaaagg atataattac acataatatt cctgtatgaa gacctgatat agatattaca
    10321 tgactagttc cagttctggt aaaaatttct ttttgttcct cagtcaaatt ttcaacttct
    10381 gctaatagta tagaatttat aaaatctgag tttttcttat ataagtacct ataagtttta
    10441 ctaatataaa actttatttc ccctattaaa gagtaaagtt tatttttatc aataatttta
    10501 tagttattta tatacactat acctttatat ccagtacttt ttatatacct tccataatca
    10561 aaatcatcat acttcatttt atctaaactt ttgaatttac ctgtaatatt aactttctga
    10621 ccaatcttca aatttttatt ttttgtataa tcatttatta gaaaaacccc taccttgtac
    10681 tgattgtatc ttttcttttc aatttttcct ttgacaactc cttgtatagt tattatctta
    10741 tcattaagtt cttcgtcgtt ttttgtgtta tttgtaatga taaatgatat agctaccaca
    10801 aacattaata taattaataa tggtcttctt atttgaatta taatcactcc catataaaag
    10861 tatattattt agattatttt caaatagaaa atttttaaac aagataaatt gttatctaaa
    10921 caaaaaaata cgtcaaaaga tttttaaa
//
LOCUS       DAEMHZ010000135        10945 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000135 DAEMHZ010000000
VERSION     DAEMHZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.135"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            438..1622
                     /locus_tag="KPK72_003127"
     CDS             438..1622
                     /locus_tag="KPK72_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490320.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1999..3108)
                     /locus_tag="KPK72_003128"
     CDS             complement(1999..3108)
                     /locus_tag="KPK72_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2490321.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3571..4764
                     /gene="nifV"
                     /locus_tag="KPK72_003129"
     CDS             3571..4764
                     /gene="nifV"
                     /locus_tag="KPK72_003129"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG2490322.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4789..6714
                     /locus_tag="KPK72_003130"
     CDS             4789..6714
                     /locus_tag="KPK72_003130"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG2490323.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6736..7731
                     /locus_tag="KPK72_003131"
     CDS             6736..7731
                     /locus_tag="KPK72_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG2490324.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            8058..8531
                     /locus_tag="KPK72_003132"
     CDS             8058..8531
                     /locus_tag="KPK72_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2490325.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8565..9059
                     /locus_tag="KPK72_003133"
     CDS             8565..9059
                     /locus_tag="KPK72_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490326.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            9156..9677
                     /locus_tag="KPK72_003134"
     CDS             9156..9677
                     /locus_tag="KPK72_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2490327.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            9866..10177
                     /locus_tag="KPK72_003135"
     CDS             9866..10177
                     /locus_tag="KPK72_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2490328.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10205..10891
                     /locus_tag="KPK72_003136"
     CDS             10205..10891
                     /locus_tag="KPK72_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2490329.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         4212 a         1183 c         1957 g         3593 t
ORIGIN      
        1 tattatgtat taataatgtt acaataaata taaataaatc aaagttactt acattacagt
       61 atattttttc acaaaatata ttgatttatt cttttaaagt ttgataattc caatgaatgt
      121 aataaaagaa ggaattttat actatacgat tattattata aaataataaa gaaaaaaata
      181 tataataata aagaaaaaat gtataatatt taatattaaa aaagatattt cataaaatta
      241 aaaaacaaag tgaatttgaa tactatttaa aaaataaaat aaatatataa aaaatattta
      301 atacaaaggt agaaataagt tgaaaatgta aagtcattgg aaaaatatag ttgaatttaa
      361 tgagcaaaaa tgttattgtt ttatcaataa agtgttaaaa taaaaacttt caatatcatt
      421 ttagggagga tgatttcatg caaaaaagtg taaagaaagc aaaagtaacg ggctctatgt
      481 tagccatttt tggtgttgca agcgttcttt ttagttcaca tgcaggagga ggatttgcta
      541 cagggaatca agaaactcag tactatgtgc agtatggatg gacagcacca cttatggcaa
      601 ttttagcaat gattatactt actgctacca tgagagaagt tatcataatg tataataata
      661 acaattgtag gaattataag gatttatttt gtgagctttg gagaccatat ccaaaacttg
      721 aaataatatg ggagatatat tactatctaa tggttttaat agctgtaagt gctgttatag
      781 caggtgcagc agcagtattt caaagtatag gagtaaatta ttttgtagct gtatttatta
      841 ttggagttgt acttcttgtc tttactatat ttggtgctat gcttgtatct aaagcagcta
      901 cagcaatgac aatagctatt ctagtatgta ctcttactat ttttattgtt ggtataaaag
      961 ctaaagtacc agaaataact gaaatattat caaatagaac gagtttcaca cctggctata
     1021 tcaagccaat acttaatact tttatttatg caggttttca atcggttgtt atacctacac
     1081 ttgcaggatg ttcaagacca cttcttaaga actcaaaaga agctactaag gcaatgataa
     1141 tttcatttgt tatgaatgca attgcattag gacttgctgt aactatgcta atgggatggt
     1201 atagagaaat tattgctgct gggcaaacaa cactccctac tctttatgtt gcaggtcaat
     1261 ctggaaatca tactatatac ataatttata atgttgcatt attcctttgt ttaatgtcta
     1321 caggagtaac tactatattt ggtcttgtaa atagatttga agaccataaa gcactttctt
     1381 tcctttcttc aagaatgaaa agaagggtat ttacagcttg tgcaataatg gtagtgtcaa
     1441 tgcttatatc acttacagga ttaagtaata ttgttaaata tggatatgga tattgtggtt
     1501 atcttggtct attcactata gtaattccat tccttacact aggacattat aagaataaga
     1561 agtttgcaaa ggaaaatcca gaagctaaat ggccagcgga attaaatgaa aatgtaaatt
     1621 agatgtagta gcaaaaaata ttgctaaaga gaatactata aataagaata ctatagataa
     1681 tatatctgca tgtttattaa gattatttgc aaaatctaat attaaacaac tataaacaag
     1741 caatataaaa ctaagaataa aagataaaaa gagggtatct caaaatggac ttttagttta
     1801 taaggcactc ttttttatat gaagtcaaat atattttcat tattttgaca ataaaagaga
     1861 ctaatcctac tttggatagc ctcttttttc tttaatataa aatagtataa cagcaaagta
     1921 tagattgata catgttttct gtagctttct atatattaca gtatttgtgt gacattggta
     1981 ttttaagcaa tctataaatt ataattctat aattcttcta ctaattttag attctatagc
     2041 cgtctctatt tcacttaaca ttcgttcatt tttagggtca ataaaagcac atgtatagcc
     2101 ttcttcacca gctctaccag ttctaccaat acggtggatg tagtcttctg gtgtttctgg
     2161 taaatcgtaa ttatatatat gacttactcc agatatatca aggccacgag aagctacatc
     2221 tgtagctatt aagtattgta tatctaaatt tctaaatgat ttcattattc tttctcgttt
     2281 tgattgagca atatcactat gaagtttttg acaattatat cctcttatag caagagcttc
     2341 ttcaagattg tcaacccttc tcttagtcct acaaaatatt attgccataa aaggattatc
     2401 ttcatctagt accttacata aggcatctaa tttttttcta tctgtagttt ctactacttc
     2461 ttgttttata ttgttaagag tgatttcttc tttttgaata gaaactacaa caggattttt
     2521 tgtgtatcta taagctaatt tttttacttg agaatttata gttgcagaaa agcatagtgt
     2581 ttgctttttg tttgaagtct cttttaaaat agcttccact tcatttttaa atcccataag
     2641 caacatttgg tctgcttcat ctaaaacaaa agtctttaac ttactaaggt ctattgtttt
     2701 tctatttaga tggtctaaaa gcctacctgg tgtagctata atcatatgaa tatttccttt
     2761 tagtttcttt atttgagaac caatatcttt tcctccatat gctgcaagaa tacttacatc
     2821 tttagattct ttaagtttca tagcttcttc tgttatttga atagctagtt ctcttgtagg
     2881 agaaagtata agtacttgta tatcatttat atctaatgat atattttcaa atatcggaag
     2941 taaaaaagct agtgtttttc ctgttccagt ttgagcttct gctattacat cttttccttc
     3001 ttttattagt ttaatacttt ctttttgtat aggtgtaggt gttgttattc ctgatttttt
     3061 tagaatattt attgtatttt cactaatccc taagcttttg aaatccattg ttatcttacc
     3121 tttcatgaaa aattttcttt tttattaacc aattaataat atcatatttg taagtatttt
     3181 tagtaaattt tttaattatt atttatatag cttaaaaata tattaagata ctttaaaatg
     3241 gaatgtaact taaattaaat tttagtagga attttatagg tttattttta aaggtttttt
     3301 gaaaataaat ctatttaaaa attaaattaa aatgcaagaa aaacataata actttcattt
     3361 ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata aatgatacaa agtaatataa
     3421 tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa aatgaataaa
     3481 aaataaaaaa ataatcataa gttagaggta tcaatgaaaa ataagactta atgtttttaa
     3541 aattaaaagt atactaaggg gggcattaat atgtgcgtta tatcaaaaga tagagcaaag
     3601 gaaataaaaa tagtagatac tactttaaga gatggagagc aaacagctgg ggttgtattt
     3661 gcaaatagag aaaaaataat gattgctgaa atgttaagtg acctaggtgt tgaccaaatt
     3721 gaagttggta tacctactat gggtggagat gaaaaaaatg taataaagca catatgtagt
     3781 agaaatttaa aatcagatat aatggcttgg aatagagcag taataaaaga tgttgaagaa
     3841 tctatttctt gtggtgttga tgcagtagct atatcaatat cagtatctga cattcatata
     3901 gaaaataaat tgagaacttc tagaggctgg gttttagaaa atatggctaa aacagttgaa
     3961 tttgcaaaga aaaatggatt atatgtttca gtaaatggag aagatgcttc aagagcagat
     4021 atagacttct taactgaatt tataaatgtt ggaaaacaag caggggcaga tagatttaga
     4081 tactgtgata cagtaggagt tatgaatcct ttttctataa agaatgcaat agaaactctt
     4141 tatgaaagaa ctaattttga tatagaaatg catacacata atgactttgg gatggcaact
     4201 gcaaatgcac ttgcaggaat agcagcagga gcaaattatg ttggtgttac agtaaatgga
     4261 ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt taatggcact taagtgtgtt
     4321 tataaatgtg atttaaataa tatagatact agaaaattta gaggaatatg tgagtatgta
     4381 gcacaggcat caggaagaat acttcctaca tggaaaccag tagttgggga taatatgttt
     4441 atacatgaat ctggtataca tgcagatgga gctttaaaag acccacataa ctatgaacca
     4501 tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca ttcaggaaga
     4561 gctgcagttg taaataagtt gagtgaatat gaaatgtata tttctccaga aaacgctacc
     4621 aagctattaa atgctataag agctacatct ataagattaa aaagaagttt aatggataaa
     4681 gaaattcttc aattatattg tgatatacta gcacatgaga aaggcacaac agaagaagaa
     4741 gcagttagag gttcatatat ttaagatata attgtttagg aggaaaatat gggagataat
     4801 atagtttata aaataattaa aaaacatata gtagatggag aagctgttgc tggaagttca
     4861 attggtataa aaatagacca aactttaaca caagattcta ctggaacaat gacatatctt
     4921 caactagaag ctatgggaat agataaagta aagactaaaa gaagtgtggc atttgtagac
     4981 cataatatgt tacaacaagg atttgaaaat gcagacgacc ataagtatat acaaacagtg
     5041 gcagataaat atggagttta tttctctaag ccaggaaatg gtatttgtca tcaagtattt
     5101 ttagaaagat tttcaacacc aggagataca cttttaggtt ctgatagcca cacacctact
     5161 gctggaggtg tgggcatgat ggctattgga gctggtggat tagatgttgc tttagccatg
     5221 gcaggaggag catactatat aaaagcacca aaagtctgta aggtgaatct agttggaaaa
     5281 ttaaataata tggtatcatc aaaagatata atattagaag tgttaagaaa acaaactgta
     5341 aaaggtggag taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag cctatctgtt
     5401 ccacaaagag ctacaataac aaatatgggg gctgaacttg gagcaactac ttcaatcttc
     5461 ccaagtgatg aaaaaacact agaatttttt aagagtcaag gaagagaaga cgcatggata
     5521 gagctaaaac cagatgcaga tgcagtttat gatgaagaga ttactataaa cttggatgaa
     5581 ttaaaaccat tagctgcaaa acctcatagc ccagacaatg tagatgaagt tgaaaatata
     5641 ggcaagatta aaatagacca agtggcaata ggaagttgta caaattcttc ttatgaagac
     5701 ttaatgaaag tagcacaaat cctgaaagga aataaagtac ataaagatgt gagtttggta
     5761 atagcaccag gttcaagaca agttatggag atgattgcta gaaatggggc tttggcagat
     5821 ataataagtg ctggagctag aatactagaa aattcttgtg gtccatgtat tggtatgggt
     5881 caatctccag gtactgactc tgtgtcttta agaactttca atagaaattt ctatggtaga
     5941 agtgggactt tgtcagcaca agtatattta gtaagtccag aagtggcagc agtaagtgca
     6001 atcaagggag tgcttacaga cccaagagaa tttgatatta aatttacaaa tttagatgta
     6061 aatgaatttt tgatagatga tagcatgata attaaacctg ctgatgtagg aagtgatgta
     6121 gaagtagtta gaggacctaa tataaaacct ttccctctta atacagagct aagtcagtct
     6181 ataggtggaa aagttatatt aaagacagaa gataatataa ctactgacca tataatgcca
     6241 tcaaatgcta agttacttcc ttttagaagt aatataccgt atcttgcaaa ctattgtttc
     6301 aatactgtag atacagaatt tcctcagaga gcaaaagaca ataatggtgg atttatagtt
     6361 ggtggagata attatggtca aggttcaagt agagaacatg cagctttagc accattatat
     6421 ctaggtgtta aaggggtaat agttaagagc tttgcaagaa tccacaaggc taacttgata
     6481 aatagtggta taattccaat ggagttttgt gatgagaaag attatgaaaa tatatctctt
     6541 ttagataatc tggagatacc aaatatttta gacaatctag gtagtggaat tttagaagtt
     6601 aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc aaaagaggtt
     6661 gatgtattaa aagctggtgg aaaattaaac tacactaaaa atcaagctaa ttaatatgaa
     6721 aagagggaat attgaatgta taaagtgaca cttataccag gagatggaat aggaccagaa
     6781 gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag tagaaataga atgggaagaa
     6841 gttaatgcag gagaggcagt tattgaagag tatggtactc cacttcctga atatattata
     6901 gatagtataa aaaagaacaa aatagctata aaaggcccaa ttactactcc tgtaggtaaa
     6961 ggatttagga gtgtaaatgt aactttaaga caggcattag atttgtatgt caatttaaga
     7021 cctattaaaa gttttaaggg gataaaatca agatatgaag atgttgattt agtggttgta
     7081 agagaaaata cagaagattt atatgctgga atagaacata aaataggtga ttatgcagct
     7141 gaaagtataa agataataac aagaagtgca agtgaaagaa tcgttgattt tgcatgtaac
     7201 tatgttaaag ataataagag aaaaaaagtt acagctatac ataaggcaaa tataatgaaa
     7261 atgtctgatg gattgttttt agatgtgttt agagaagttg catcaaaaca tggtgtagaa
     7321 tatgatgatt tgattgtaga tgcagcagct atgaacttag ttttaaatcc agaaaattat
     7381 gatgtaatgg taatgccaaa tctttatgga gatatactat cagatttagg tgctgggctt
     7441 gttggaggtt taggtataat tccaagtgca aatattggta aagattgtgc gatatttgaa
     7501 gctgttcatg gaagtgctcc acaaatagca ggtcaaaata aggctaatcc aacagcactt
     7561 attcaatcat cagtaatgat gcttagatac ttaggtgaat atgaaaatgc acaaaaaata
     7621 gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga tttaggtggc
     7681 tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt atattgtata gattttattt
     7741 agggaaaaag taaaatatag attgaagaaa agaggctttc tcaaaataga gataagcctt
     7801 ttttattgga atgataaaat atatttgatt ttatacaaaa gagagttcct cataaatgaa
     7861 aaagttaatt ttgggatatc cattttaatt ttaaaattaa ttgattaata cgcaaagaaa
     7921 gattaaataa atttattgtt tttaattgaa ctttcaacca aaatatggta aaatttaaaa
     7981 aaagttgaac tttcaactaa aaatgaatat attgattatg tgaaattcta taaaacagaa
     8041 actatttgac tttagccatg gtagtttcag tggaggtgtg tttgattaag actttagata
     8101 gtaatatatt aagagaagta ggtactttat ctagagcagt aaattcaata aatgatatta
     8161 agtacaagga attaaagtta caaaaaggac aatttacatt tttgacaaga atctgtgaaa
     8221 atcctggtat aaatcttgtg gaattatcaa atatgttaaa ggtagacaag gctacaacaa
     8281 ccaaggctat acaaaaatta ataaaggcag gatatgtaga taaaaagcag gataagtttg
     8341 ataaaagagg atataattta actccaactg ataaatcttt ggaagtttat gagttgataa
     8401 ttgaagaaga gaatagaagt atagagatat gttttgataa ttttacagat gaagaaaaac
     8461 aagtggtaac taagttatta gaaaaaatga gcaaaaacgt agaaaatgaa tggtttaaag
     8521 taaagagata gtaaataaat acattaactt aaggagatat ttttatgatt agaaaagcga
     8581 atatgaatga tttagaaagc attatgaaaa ttataaaatc tacagtggaa gaaatgaaga
     8641 cttataataa tacacaatgg gatgaaaact atccattaga aaaagacttt gtttcagata
     8701 ttaagaaaca agatttatat atttatgaag tggatggaga agtagcaggg tttatatgtc
     8761 ttaattatga agagccagaa gaatatttag gcttaaattg gtcatctaat aaaaaagcaa
     8821 tggtaataca tagaatggct gtaaatccaa actttagaaa gacaggaata gcatctaagt
     8881 tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt atcatactta aagagcgata
     8941 catattcaat aaactcaaaa atgaatttat tacttacaaa gtgtggtttt ataaaaatcg
     9001 gagaaatgag ttttttagga aaggagaaat ctttctactg ttatgataaa atattataaa
     9061 gagcatttat aatacaaggg gtaaataata gattttcagc attagtaagg gcataatata
     9121 acctagtata tgctattatg aaagggatga tttatatgat tggatttcta aagaaaagaa
     9181 gaagtattag aaaatacaag gatgttgaag tagaaaaaga aaaattagat aaaatattaa
     9241 aagctgctct tttagctcca tcatcaaagg ggcttagaac atgggaattt atagtggtag
     9301 atgataaaga aaagctaatt aatttatctc aatgtagaac taaaggtggt ggatttttct
     9361 taaaaaatgc acctttagct attgtaatta ttgcagataa agaaaaaaat gatgtttgga
     9421 tagaggatgc aagtatagct gcttcataca tacaacttca agctcatgaa ctaggtttag
     9481 gttcttgttg gatacaagta agaaatagaa tgcatgatga taatatagaa gctgataagt
     9541 acattagaga agaattaaaa gtacctagca agtacagtgt agaatgtata atttctatag
     9601 gatatcctga tgaagaaaag aaagcatacg gtgactctga tttagactat aaaaaggttc
     9661 attttaacaa cttttagtat ataatatgtt gtattaagac attatatttt tttctattgt
     9721 taatatatac ttaaaattgt aaaaatatat ctgaatttta ctaacttatc taggggttat
     9781 gtacaaggat tttaaagata aatatagaaa ttaatgttta taatcgatac ataacttgag
     9841 taacaaaatt taaggaggtt aaagtatggg tatatttaaa acaaaaatgg atgaggactg
     9901 gaaagtaaac tacattaaag agtttaatga aatgagagat tcttatgaga gtaaacttca
     9961 aaaaaaacag tttgaggtag atagtttgaa atcagaattg gatagactaa gaagctataa
    10021 aaatagcctt aagccaaaag aaaaacaaat tactgatgat gatataaata atataaagag
    10081 tttaagaaga gatggcttaa gctataaaga aatttcaaat caaacaagtt ggagcaaagc
    10141 aactgtaagt agggtattaa atggacttta cgattagcat atacatgggg tgatttaaat
    10201 tgggatgaag tatagttaca aaaaatgcat aatagattct attttattga tgtttatagt
    10261 tcaaatatta aggatgattt tgaattatgt acttctaagt caatttgaat ttactcttga
    10321 aaattttaat attataaact taatttcttt tacattagtt ggtttatcgt taattttatt
    10381 tttaaaagat aattctttgt ataacaaggt tagaaataga aaaatcacag aagcttttga
    10441 agaaaataaa aacaatatac ttattgaaaa gtgtaagctg atactttttg ttgtggtttt
    10501 atctttagca ataattgtaa cttattgtac taagggatat gtacttttta atgtaactat
    10561 gatgactttg tctgtattga tagtcccaat atttgaagag ttatttttta gagaatatat
    10621 atggaactat ctaagtaatt ttataaagtc aaagagtaaa attatctgta taacatctat
    10681 actatctgga atttataata taggatacat agatgttata agaaattatg taatattgta
    10741 taacaattca agctacacct ttgaagttat tatttcaaaa attatgatag gtactgtttt
    10801 tggtattgta cttggattag ttaagtatag gtttagagat gtagggtttt gtatattatt
    10861 gaggagctta tttgctatat ttattaggta gatagtataa tcggtttata aggttgtttt
    10921 aaatttaatt ttaaatcaac cttat
//
LOCUS       DAEMHZ010000136        10911 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000136 DAEMHZ010000000
VERSION     DAEMHZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.136"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            208..1530
                     /gene="dnaA"
                     /locus_tag="KPK72_003137"
     CDS             208..1530
                     /gene="dnaA"
                     /locus_tag="KPK72_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG2490330.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1772..2878
                     /locus_tag="KPK72_003138"
     CDS             1772..2878
                     /locus_tag="KPK72_003138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG2490331.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3015..3221
                     /gene="yaaA"
                     /locus_tag="KPK72_003139"
     CDS             3015..3221
                     /gene="yaaA"
                     /locus_tag="KPK72_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG2490332.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3239..4354
                     /gene="recF"
                     /locus_tag="KPK72_003140"
     CDS             3239..4354
                     /gene="recF"
                     /locus_tag="KPK72_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG2490333.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4355..6256
                     /gene="gyrB"
                     /locus_tag="KPK72_003141"
     CDS             4355..6256
                     /gene="gyrB"
                     /locus_tag="KPK72_003141"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG2490334.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6276..8702
                     /gene="gyrA"
                     /locus_tag="KPK72_003142"
     CDS             6276..8702
                     /gene="gyrA"
                     /locus_tag="KPK72_003142"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG2490335.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8771..9067
                     /locus_tag="KPK72_003143"
     CDS             8771..9067
                     /locus_tag="KPK72_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2490336.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9067..9327
                     /locus_tag="KPK72_003144"
     CDS             9067..9327
                     /locus_tag="KPK72_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG2490337.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9339..9656
                     /locus_tag="KPK72_003145"
     CDS             9339..9656
                     /locus_tag="KPK72_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG2490338.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9681..10067
                     /locus_tag="KPK72_003146"
     CDS             9681..10067
                     /locus_tag="KPK72_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2490339.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10067..10840
                     /locus_tag="KPK72_003147"
     CDS             10067..10840
                     /locus_tag="KPK72_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG2490340.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4442 a         1129 c         1994 g         3346 t
ORIGIN      
        1 aatcaccatt tattgtatgt ttatgttatt tataacttta tctacatatt gtttatatct
       61 tttcatattt ttttaataat ttatactatt attaatttat caacaccatt atccataaat
      121 ttagttattc tgtaaaattt atatatattt ttgcttttct gttaataact ttgtggaaaa
      181 tattaattat tctgttcgga ggtttatatt atggatatag tttctttatg ggacaaaacc
      241 ctacaattaa taaaaggtga cttaacttca gtaagtttta ataccttttt taaaaacatc
      301 gtaccattaa aaatacatct taatgattta atcttactgg ctcctagtga ttttaataaa
      361 gatatcttag agaatagata tttacattta attgaagatg ctattagtca actgtcatta
      421 aaaaaatata atattaaatt tgtattgagc gaaaaagagg ttgctgattt aaattctgat
      481 agcacagatt taaattatag agttctttac cccaatctaa atccaaaata cacatttgat
      541 acattcgtta ttggtaatag taatagattt gcacatgctg catgcgttgc agttgcagaa
      601 tctccagcta aagcttataa cccacttttt ttgtatggag gagttggatt aggtaagact
      661 cacctaatgc atgctatagg tcatcatatt gttagtcaaa aaaaagactc aaaagtagtt
      721 tacgtttctt cagaaaaatt tactaatgaa cttataaact ctataaaaga cgataaaaac
      781 gaagaattta ggaataaata tagaaacgta gatgtattac taatcgacga tattcaattt
      841 atagcaggaa aagaaagaac tcaagaagag tttttccata cattcaatac tcttcatgaa
      901 gcaaacaaac aaataattat atctagtgat agaccgccaa aagacatacc tacactagaa
      961 gataggctaa gatcgagatt tgaaatggga cttataactg atattcaagc acctgacttt
     1021 gagacaagaa ttgctatact tagaaaaaag gctcaactag aacgtataga tgtgccaaat
     1081 gaggttatgt catatatagc aaaaaatatt aagtcaaaca taagggaact agaaggtgca
     1141 ttaactagag tggttgctta ttcttctctt agcaataggg taatatcttt tgatcttgct
     1201 acagaagctt taaaagatat tataacaact tctaaaaatg aagaaataaa tgttctcaga
     1261 ataaaagaaa aagtatcttc tgtattcaat ttaaagatgg aagattttaa ttctaaaaaa
     1321 agaacacgtt caatagctta tccaagacag atagctatgt atttaacgcg cgagcttact
     1381 gatttatccc ttcctaaaat aggtgaagag tttgggggta gagaccatac aacagtaatt
     1441 catgctcatg acaaagtatc taaggatata gaagaaagtg aagaaattaa gactaaaata
     1501 gacaaaatta tatcagattt gaagggataa tttatacaca attgtatgtt tatatctctt
     1561 gaataacctg ttaatacttt ttttgaacaa aaattattaa caaattatca agtctaaaat
     1621 aacaaagata ttaacatctt atacacatgt taatatcttt gtatttataa tgttatacat
     1681 acttattaac attatcaaca acacctacta ctattactac tatgatttta acttattttt
     1741 atatataaaa agccagagga ggttatccac attgaaaata atttgtaatc aaaaaatcct
     1801 agcaaataga attggaattg ctcaaaaagc tattaatgga aaaacaacaa tagaattatt
     1861 aaaaggtata cttatatcta cagaagaagg tcaattaaaa cttactggat atgatgctga
     1921 aataggaata gaaacttatg tacaagcaga aatcatagaa aaaggtgatg ttgttgtaga
     1981 tgcaaggcta tttggagata taattagaaa attaccagat tcctttgttg agatagaaac
     2041 tgattcagaa aataatattt acataaattg tgtaaattca agatttaaaa ttaaaggtta
     2101 tgcagcaaaa gaatttccta aattaccaga attaaatgaa gaagatttat atagcatacc
     2161 tcaagaaatc ctaaaaaata tgattaaaca aactgtattt gctatctcac aggaccaaac
     2221 aaaacctgtt ttaatgggag aacttttaga aattgtagat agaaacttaa atttagtagc
     2281 aattgatgga tatagattag ctgtaaaaag ttgttcggtt gatagtttaa ctgaaaatat
     2341 aaaagtcata attcctggaa agacacttat agatgtaaat agtttgcttt ctggagagga
     2401 taatgttaaa gttggattta atgaaaaaaa tgctattttt ataattaatg atacgaaaat
     2461 tattacaaga ttgcttgaag gagattttat tgattataaa aaattattgc caagagaaca
     2521 taacagcaga gttaaattaa atactaagga acttttaaat agtatagaga gagcgtcttt
     2581 attgtctcaa tcagaaaaaa ataatcttat aaagttatct ataagagata aagtaatggc
     2641 aattacttct aatacagaaa aaggaaatgt gtatgaagaa gttgaaatag atttagatgg
     2701 agattaccta gatatagctt tcaattctag atattttatc gaaggtttga aaaatataga
     2761 taatgaggaa atatttatag aatttactac aaatgtaaat ccttgtataa ttaaaccaac
     2821 agatgatgtt aattatattt acttactgct tccagtaaga atatcatcaa atatatagca
     2881 gacatgaagg agttgtcttt tgtagaaaat ttttctaatc ttagacaact ccttttataa
     2941 gcatatttat ggtataatat acttttagtg tatcaatagg aatttgttta aaatagtgac
     3001 aaggaggaac tattatgact gaaataacta tagaatcaga atatataaag ttggatcaat
     3061 ttcttaaatt agcagagatt gcatcaacag gaggtcatgc aaaattttta attcaagaag
     3121 gtttagtgac agtaaatgat gaaatagaat taagaagagg aaaaaagata aaatcaggag
     3181 atatagttga aatagaagga actaaaataa aagtattgta aaaaaaggag aactttttgt
     3241 gaaacttaaa agtttgcaac ttgttaattt tagaaattat aaaaagttgc atctggaatt
     3301 taatgggaaa gtaaatctac ttgttggtaa aaatggtcaa ggaaagacaa atatagtaga
     3361 atctatttat atgctttctt ttggaaaatc atttaggact aacaaagata aagaaatggt
     3421 tagatttaat agtgaaaact tatatattgg aggaagtttt tcaaaatata ataaatatag
     3481 tctaatagag ctaattattg gaaaagataa aaaagggata agaataaata aagttccttt
     3541 acaaaagata caagaactac ttggcaactt aaatgttgtt atattttctc ctgaggattt
     3601 aaggttggtt aaagagggtc ctaaagaaag aagggcattt atagacaaag agattagtca
     3661 gattatacct aaatattata aatatcttac caattataat aaaacacttt ctcaaaggag
     3721 tagggtgctt aaaaatatac atgttgatga agctttatta gatgtatatg atgatacgtt
     3781 agcaaagtat ggaagttata tttatatttt gcgtagagac tttataaaaa agatagctaa
     3841 tatatctgaa aatatgcata tgaatttgac taatggagtt gaaagattat caattcgata
     3901 taaaaatcaa ataaatataa ctgatgaaga tactatagat acagtatata ataaattttt
     3961 agccaagttg tcatcgaata gacctaatga tatagaatct aagactacca gatatggtat
     4021 acacaaagat gacttaaata tattcataaa tgatttagat gcaagacttt ttggttcaca
     4081 aggtcaacaa agaactgctt caatatcatt aaagttatca gagatagaat taataaaaaa
     4141 tgaagttgaa gaatatccag tattaatttt agatgatgtt tttagtgagt tagatgaagc
     4201 aagacaaaaa cttttagtaa ataatttaag caatgttcaa atgtttataa caagtgctga
     4261 agtttcacat aaaaaaatat ttgatgaaaa aaatgttaca atatttaata ttgaaaatgg
     4321 cgatgttatt agcatagaga atggaggaaa ttaaatgaaa caagaatacg gtgcaagtca
     4381 gatacaagta ttagagggat tagaagccgt tagaaaaaga ccaggtatgt atattggtag
     4441 tacaagccct agaggtttac atcacttagt ttatgaagtt gtagataata gtatagatga
     4501 ggctttacaa ggatattgtt cagacatata tgtatctatt aatgaagatg gcagtgtttt
     4561 agtaaaagat aacggaagag gtatacctgt tgaaatacat cctaaaactg gtaaatcaac
     4621 tttagaaaca gtattaacta atcttcatgc aggaggaaag tttggaggcg gaggatataa
     4681 agtatctgga ggtcttcatg gagttggtgt ttctgtagtt aatgcattgt caaaatggat
     4741 ggtagcagag gtttatttaa acggaaagat atataagcag acatatgaga aaggattgcc
     4801 aacatctaag cttgaagtag ttggagaatc tcaagataaa ggaacaatga ttcaatttat
     4861 gccagatgag acaatatttg atgaaataga gtttaaatat gaaacattgg agtataggct
     4921 tagagaactt tctttcttga ataaaggaat aaaaatagta tttgaagata aaagagaagg
     4981 acaagaaaaa agaaaagaat ttcactatac aggtggtttg gtagaatata ttaagtattt
     5041 aaataaatct agaactggta tacatgatga catagtttat atagataaaa aggttgatga
     5101 ttgttttgtt gaactagcta tgcaatatac agatggttat acagagaaca tatattcttt
     5161 tgcaaacaat ataaatacac atgaaggtgg ttctcattta agtggattta aagcagctct
     5221 tactaaaact gtaaatgatt atgctaagag aaataaattt ttaaaggaaa atgatgttaa
     5281 cctacttggt gaagacataa gagaaggtct tacagcagta gtatcagtta aattaccaga
     5341 acctcaattt gaaggtcaaa ctaaaacaaa gctaggtaat agctttatga gaggtatagt
     5401 tgatagtgta acagttgatg aactggggtc tttcctagaa gaaaacccat caacagcaag
     5461 aatcatagtt gataaagctt taagagcaca aagagctaga gaagctgcaa aaaaagcaag
     5521 agaattaaca aggagaaaaa gtgtattaga aagtacatct ttacctggaa aacttgcaga
     5581 ttgtgcagaa aaagatccat ctaaaagtga aatattctta gtcgaagggg attcagcggg
     5641 aggttcagct aaacaaggta gagatagaaa tagtcaagct atacttccat taagagggaa
     5701 aatacttaat gttgagaaat caagactaga taggatacta tcttcagatg aaataaaaaa
     5761 tatgataaca gcttatggtt gtggtattgg agaagatttt gatatagata aggctaggta
     5821 tcataaaatt ataattatga ccgatgctga tgtagacgga gcccatataa gaactttatt
     5881 attaacattc ttctttagat atatgagacc tcttatagat gaggggtatg tttatgcagc
     5941 acagccacct ttatataaag tgacaaagca aaagaaggaa cattatgttt actcagataa
     6001 agagttaaat atcttattag atgaaattgg aagaaatggt gttgaacttc aaaggtacaa
     6061 aggtcttgga gagatgaatg cagagcaatt atgggaaaca actatgaatc ctgagacaag
     6121 aactttatta caagttactg tagaagatgc tgcaattgca gatgaagtat tttcaatgct
     6181 tatgggtgat aaggttgctc caagaaaaga atttatagaa gaaaatgcaa gatttgttag
     6241 aaacttagat atatagagag aggggattag tatacatgga agaaaataac aaaatactcc
     6301 ctattgaaat agcggaagaa atgaaaaaat cgtatattga ttattcaatg agtgttatag
     6361 ctggacgtgc tcttcctgat gttagagatg gtttaaagcc agttcataga agaatattat
     6421 attcaatgag tgagttaaat ttaactccag ataaaccata caggaagtca gctcgtattg
     6481 ttggggacgt tttaggtaag taccatcctc atggagatac tgctgtttat tatgctatgg
     6541 taagaatggc acaagatttt tcaactagag cacttttagt agatggtcat ggtaactttg
     6601 gttctgttga tggggattca ccagctgcta tgcgttatac agaagctaaa atgagtaaat
     6661 tatcattaga actactgaga gatattgaaa aggaaactgt agactttaaa ccaaactttg
     6721 atgagtcgtt aaaagagcct tcagtattgc cagctagata tcctaattta ttagtaaatg
     6781 gctcaaatgg tatagctgtt ggtatggcaa cttcaatacc tccacataat ttagcagaag
     6841 taattgatgc aactgtatat ttgatagata atccagagtg tagtgtagat gatttaataa
     6901 aatttgttca aggaccagat ttccctacag ctgcaattat aatgggaaaa gaaagtatag
     6961 cagaagcata cagaactgga agaggaaaag ttaaagttag gtctagagct tttatagaag
     7021 aactaccaaa aggaaaacag caaataatag ttacagaaat accttatcaa gtaaataagg
     7081 ctaaactggt tgaaagaata gcagagttag ttaaagaaaa gagaatagaa ggtatatcag
     7141 acctaagaga cgaaagtaat agaaatggta tgagaattgt tatagaatta aagagggatg
     7201 ctaatgctaa tatagtatta aataatttgt ataaacattc tcaaatggaa gatactttta
     7261 gtataataat gcttgcactt gtagatggtc agccaagagt tttaaatctt aaacaaatat
     7321 tatatcatta tattaaacat caagaagatg ttgttacaag aagaactaaa tttgaactaa
     7381 ataaagctga agcaagagca catattttag aaggattaaa gattgcttta gataatatag
     7441 atgctgttat aagcttgata agagcttcaa agactgggca agaagctaag ctaggtttaa
     7501 tagaaaaatt caaattaact gaaatccaag cacaagctat attagatatg agacttcaaa
     7561 gacttacagg tttagaaaga gataagatag aagctgaata tgaagattta atcaagaaga
     7621 taaatagatt aaaagagatt ttagctgatg aaagattact tttaaatgta ataaaggatg
     7681 aaattacaat aataaaagaa aattactctg atgagagaag aacagaaata agacatgctg
     7741 aaggcgaaat agatatgaga gatcttataa gtgatgaaga aatagcaata actcttactc
     7801 actttggata tataaaaagg cttccatctg atacttataa gagtcaaaaa aggggtggaa
     7861 gaggtatttc agcacttaca actagagaag aagattttgt aaggcacttg gtaactacaa
     7921 ctactcatag tagactatta ttctttacaa ataaaggtag agtatttaaa ttaaatgcat
     7981 atgaaatacc agaaggtaag agacaggcta aaggtactgc tatagtgaat ttacttcaat
     8041 tgtcagcaga tgagaaaatt gctactctaa tacctattga tggtaatgat gaaaatgaat
     8101 atttattact tgcaacgaaa aaaggtattg ttaaaaagac taagagagaa gagttcaaaa
     8161 atataaataa atctggtctt attgctatag gtttaagaga tgatgatgag cttattggag
     8221 tagaacttac agatggaaaa caagaagtac ttttagtaac taaagaaggt atgtctataa
     8281 gatttgatga aaatgatata agatatatgg gtagaacagc aatgggtgta aaaggtataa
     8341 ctttaagtaa agaagatttt gttgtgtcta tgaacctttg cagtaaaggt acagatgtgt
     8401 tagttgtaag taagaatggt tttggaaaga gaacaaatat agaagagtat agaagtcaaa
     8461 taagagctgg taaaggaatt aaaacttata atatatctga aaaaactggt acaattgtag
     8521 gtgcagatat ggtcaacgaa gatgatgaga taatgattat aaattctgat ggagttctta
     8581 ttagaataag agtcaatgaa atatcactgt ttggaagagt tacaagtggt gttaaattaa
     8641 tgaagacgaa tgatgaagtc aatgtagttt cgattgccaa aataaatatt gaagaagaat
     8701 aggataaata tcctattctt tttgtataat gtagttagat aaaaatttaa aattgaaagg
     8761 agtaattgtt atgtgcaata aaagaagttg taaaaaaggt ggatttttat tattaggagc
     8821 tattttaggg tttatatttg gtatgttttt tgcacctaaa aaaggttctg aactaagaaa
     8881 agaaacaaaa gagaagttta atgatgtcaa agaaaatcca aaagaagttc tacatgaaac
     8941 atttaatgat gttaaagaga gaataataaa tttagtggat gatgataata atgaagaaga
     9001 tataaagatt tcagaagaag atatagtaat aagtaaaagc tttgatgatg agggagatgt
     9061 taactaatga atgcatgggg gtggcaaatt ggagcagtgt tagtaggaag ctctgctctt
     9121 atagtagcaa tttatttagc taaaactcta aacagcataa ataaggttgt tgaaaaggca
     9181 tataagattg tagattataa tgaaagacat atacaagata ttgttgaaaa tgcatcatca
     9241 atatcaaagg gaatagatga tatagtttat gtaattaata aaatattgag tataggaaat
     9301 atatttaaaa ttattaaaag gaaataggag gtttttacat gtctatgaat attgattcaa
     9361 atttagattc acaaaataaa ttttggaatg tttgtcttga tggagaactt gatgtttcta
     9421 cagcagataa actaaaggaa catttacatg ctttaataga aaaaaatatg ttagatgtaa
     9481 agattaatct taaagattta gattatatag actcaacagg cttaggagct atgataggtg
     9541 tattaaaaaa attaaaaata aatgaaaaag agatatacat agtaaatcct aagagtaacg
     9601 taagaaagat ttttactata acaggtctag ataaaatatt taaagtggag ggataaactt
     9661 tggcttgtga gactataaag atggaaatta ctacaaatcc agattatgtt agtataataa
     9721 gattaacagc atctggaatt gccaataaga tgggttttcc aatagatgat atagaagata
     9781 taaaggtagc agtatctgaa gcatgtacaa atgcaataaa acacagtaaa gatgatgtgt
     9841 ttaatatagt atttaatatt ttagataatg caattaatat agaaattcaa gataatggaa
     9901 aaggatatga tgttagctca ataagtacac cagatttaga gaatccaaaa gagagtggtc
     9961 ttggattgtt tataattaaa actttaatgg atgatgtaga tattgaatca gaacataatc
    10021 aagggacaaa aataaaaatg actaaatatt taggagttga tatttaatga aaaatgtagc
    10081 taatgctaca cattacctaa atatggatac aaaagagcta tttaatttat ataataaaaa
    10141 taaaaatgta gacattagaa atattcttat tgaaagacat ctgtatttag caagattgtt
    10201 agctaagaag tatataaaca aaggtgttga ttttgaagat atatatcaag ttgcttcatt
    10261 ggcattaatt tatgctattg atagatatga tgttgagaaa ggatttgagt tctccagctt
    10321 tgcaacacca actatagtag gagaaataaa aaagtatttt agagataaag tatggacact
    10381 tagagtccca agaagaatac aggaattaag caaaaagata agtgatgcga agattaaatt
    10441 agaacaagaa aataagaagc atccaaaggt aaaagatata gctgattata taggcgttag
    10501 tgaagaagat gttttagagg ctatggaggc ttcttatgga tatcaaccaa tgtctttgga
    10561 ttcatcaagt aatgatgatt cagaggataa agatataact cttattgata agattggaaa
    10621 agaagaaggt aactttggaa gtatagagta tgaagatttt attaataagt ttattgaaac
    10681 tttaaatgaa ttagaagtta aaatttttaa agatagattt ttctttgata aaacacaatc
    10741 tagtatagct aaagaactag ggatatcaca gatgactgtt tctagactag agaaaaaggt
    10801 agtagagaag ctgaaaaaag aatatgaaaa aaatttataa aaaaatataa aaaaagtatt
    10861 gacctactgt tttaaagatg gtatagtatt acttgtcctt gaaaaaagga a
//
LOCUS       DAEMHZ010000137        10605 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000137 DAEMHZ010000000
VERSION     DAEMHZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.137"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..966
                     /locus_tag="KPK72_003148"
     CDS             <1..966
                     /locus_tag="KPK72_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2490341.1"
                     /translation="ELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYKYILFSFVI
                     ISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKK
                     VNTDLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYD
                     LVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISK
                     NTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNI
                     KKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            959..1606
                     /locus_tag="KPK72_003149"
     CDS             959..1606
                     /locus_tag="KPK72_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490342.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1735..2670
                     /locus_tag="KPK72_003150"
     CDS             1735..2670
                     /locus_tag="KPK72_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490343.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINDLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSIIDKNDSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2684..3781
                     /locus_tag="KPK72_003151"
     CDS             2684..3781
                     /locus_tag="KPK72_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490344.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSCFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGDIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3784..4848
                     /locus_tag="KPK72_003152"
     CDS             3784..4848
                     /locus_tag="KPK72_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490345.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5092..5589
                     /locus_tag="KPK72_003153"
     CDS             5092..5589
                     /locus_tag="KPK72_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2490346.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5764..6012)
                     /locus_tag="KPK72_003154"
     CDS             complement(5764..6012)
                     /locus_tag="KPK72_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490347.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6078..7094)
                     /locus_tag="KPK72_003155"
     CDS             complement(6078..7094)
                     /locus_tag="KPK72_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2490348.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7152..7919)
                     /locus_tag="KPK72_003156"
     CDS             complement(7152..7919)
                     /locus_tag="KPK72_003156"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG2490349.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7987..10176)
                     /locus_tag="KPK72_003157"
     CDS             complement(7987..10176)
                     /locus_tag="KPK72_003157"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG2490350.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3701 a         1610 c         1201 g         4093 t
ORIGIN      
        1 gaacttatat taatttttat actttgtaat tatacaaaaa cttttaattc gatttacttt
       61 gttcctttaa tattagatat atcattttta ataaaggaaa aatataagta catattgttc
      121 tcttttgtta taatatcttc tcttattatt agtttagata aaaatatata tttggcactg
      181 gagtcaagct ctatattgct aattataact gtactttcta tatatataga aaatgagaat
      241 ttatcaaaat tatacagtca aaatctttat gataagctta gaatttctaa agatgaatta
      301 aaaaaagtta atactgattt ggaaacttat gcaagttcta tagaagaatt agcaatttta
      361 aaagaaagaa atcgaatatc aagagaaata catgatagtg taggacattc tctttctaca
      421 acaataatac aattaaatgc tatagaaaaa ctcttaaaag acaaacctct aatttatgac
      481 ttagtacatg aacttagaga atttgtaact gaaagttttc aagatgtaag aagggctata
      541 tctgagttaa aacctgttga atatgaaaat taccaaagtt tatttaaaat caaagaatta
      601 gttaaaagct ttattaagct tacaaatatt aatgttaaat taactatttc aaaaaatact
      661 tggaacttat ccagaaatca atctatagct ttatatagac tcatacagga atccctttcc
      721 aactcatcta ggcatggaaa ggcaactgaa atcagaattt ttattacatt caatacttca
      781 aatcttataa ttactattag tgataatggt ataggatgtg gcaatataaa aaaaggaaat
      841 ggacttaata gtatcaacga gagaatatat gaattaaatg gtaaagttga atttgataat
      901 tctaataatg gtttcacaat tagagcttct tttcctaaat tttcaggggg tgattttttt
      961 gaataaaatt aaagttctta tagtagatga cgaaaaatta attagaaaag gtcttaaaat
     1021 tatattatca tcttataatg atttagaaat tgtaggagat gcttctaatg gatatgaagc
     1081 tttagaattt tgtaaaatga atgatgtaga catagttctt atggatatac gaatgaaagt
     1141 atgtgatggg gttctaggta caaggcttat aaaggaatat aataattcta taacattatt
     1201 aattttaaca acttttaatg atgatgaata tataaaagat gctatgaagt ttggtgcttc
     1261 aggatacctt ttaaaagata gctctgatga agtattgcat gaagggattc gttcttcatt
     1321 ttttggcaat attgtgcttg ataaaagtgt agctgaaaaa atcatgacct ctgaaaaaac
     1381 ggttaaacaa gagcatcttt atgacatgta caatttaaca gaaaaagaaa tatctataat
     1441 tagactaatt gcaaatggac ttaataataa agaaataagt caagaactat tcttatctga
     1501 aggtacaata aagaataaca taactaatat tttagctaaa ctagaacttc gtgacagaac
     1561 tcaactagca atctttgctt tcaaaaataa aattgtaata gaataaatct aaaaatataa
     1621 agtgacttaa gtcacttttt ttagtgactt atagtaatag aattataatt cacaattctt
     1681 tattataata ttatagcttt ttaacttata caaaattatt ttggagggat tactatggat
     1741 atagtcagag taaataatat aacaaaaaga tttaatgata aattagtact tgataacatc
     1801 agcttttctg ttaaaaaagg tgagatattt ggacttattg gcccaaatgg tgcaggtaaa
     1861 tctacactta taaatataat tacaaatctt atgttaccaa atagtggtag tattcaaatc
     1921 aatgatttag atttatcaaa agattatata aaagcaaagt ctataatagg gttagttcca
     1981 caagaattag caattataga aactcttact ccttttgata acttagagta ctttggagct
     2041 ttctacggtt taaagggaaa actacttaaa gaaagaatca ttgaagcatt agaagttaca
     2101 ggtcttactg aagtcaaaaa gaaaaaggtc aaaaaattat ctggtggtat gcaaaggaga
     2161 ttaaatattg gaatagcact cttaaatcat cctaaaatat taatattgga tgaaccaact
     2221 gtaggtgtag acccccaatc aagaaatcat attttcaatt ttataaaaga tatatctaaa
     2281 aaacatgaaa ctactgtaat atatacttcc cattatatgg aagaagttga acacctttgt
     2341 tctaaaattt ttattatgga tgaaggtaaa gaaattgctt ttggagataa tgattattta
     2401 aaatcactcg tatctacaaa tacaaaattg attatggaaa taaaaaatat aaatgcccaa
     2461 cttatctttg atttaaaaaa tactaaaggt gttatatctg ttttagaaaa taattcatta
     2521 ttagaattgg atatagataa aaaattacaa cttacagata tactctctat aattgacaaa
     2581 aacgattcaa aaataatgaa aatttcttat gaagaaccta gtctagaaga tgtattttta
     2641 aatctaacag gcaaaaattt aagggactaa tggaggtgat tttatgaaat tatacatgag
     2701 tgtaaaaact atgctaaaga gtttaaaaag ctcctttata cttaatttaa tatatttttt
     2761 agctttgcct ctgattttat cttgttttct tgggatggtt acagaaagca tgtttcaaaa
     2821 ccctataaaa acagaatcta cccccattgt tatatatgac aaagataaca caagattatc
     2881 aaatgatttg acaaagtatt tgaaaaatga tttatcttat atattaacag ttaaaaaaga
     2941 tgattctaag gcagaattaa agcttactat acctaaaggc tatgagagta gtttattaaa
     3001 tgaaaaatca aatactctaa acatagaaaa acttggaact cgtgatgata ttgcaatatt
     3061 gcttcaagat atattagata cttaccatga aaaattttat ttaaataatt ctcagaaaat
     3121 aagttcagaa gatttttcta aattatttaa taaaaattct attgatactt ctataataaa
     3181 taacaatgtt aaacagagct cttatgaata ctttgcttta gtttcactag gatttcttgt
     3241 aataattttt ataatgaaca atattttatc aaattatata agtgaatcaa agggactttc
     3301 aaaaagactt tattctatgc ctataactag agtacaattc ttaatttatg actttgtagg
     3361 tttatggatt tattcattta tattcttatt attatatgtt ttattcttta gaatacttgg
     3421 aataacattt aaaggtaatt ttgccatatt gctcctactt tgtgctttat catcttattt
     3481 tatgacttca atatctacct ttgtaaccag cttttttagc aaaaaatacg gtacagcaat
     3541 agtttatgca ttactctttt tacagacaat ttttggagat atcttcagta tgataagtga
     3601 tacatttact aagcttacaa gtttatctcc tacctattta ataggagaat tgtttagtaa
     3661 ttatgaaaca tttaaaacta tagattcaat aggagattta attttcacat gtttaattat
     3721 ctctacaata ttaattgcac ttgctattgt aaaggaaaaa tataaatgga gggaggttta
     3781 ataatgatta gattaactat tgcaaatata aaaagatatc taaaaaatca tactctttta
     3841 attaatatgg ttatgcttcc tattatacta attttttcac ttaatttttt tatcaacaac
     3901 tcgggaaatc aatcaatgta cttttctcca gtagcaattg tatctgactc ttcaggaaag
     3961 tatgaacata agttaataaa tagttctaaa ttaaaagaaa attcatttag attaaatgaa
     4021 caagataagg ctatgaatct attaaaaaat aataaggttt ctgctgtatt cgttttagat
     4081 aaaaattttt caaatagtat agataaatta caacgacctg tagttaaatg ctttaaaatt
     4141 gaaaatggtg gtggtagtct ttgggcagaa tcacaaatag aatcttttat tactaaatcg
     4201 ttaaaattga aagtggataa aaatatagat gataaattga caaagacaaa tataattgat
     4261 aacaaaccag ataagaataa aggttcattt ttagtagtct tcttgatatg ctattttatg
     4321 tatattaatg cggcacatct tgcttctgat ttatttacat taaaaaaatc aaatgtgcta
     4381 aaaagactta tctctacaga taataaagat ataaaaataa ttttcagtat atttttagga
     4441 ctatttttta ttcaatcaat tgtatacact tttgctctat tatgtttttc aattatagaa
     4501 gactttaatt tgagtctaaa tgtattgatg attgtactag caaatagttt tgtttccact
     4561 ggatttgtat tttgggtagc tagagtattt aaaaatgaaa gttctataag tcttatatct
     4621 acattttatt ctttaatagg tctagctata tctatctcta gtctaatacc atcaatggat
     4681 aaattatcct ttatgactaa tctttcaaag ttcacaccat tttattggac tatagatgct
     4741 attaaaaaca atggtagtat tttaataaat ataatagctc taatattaat tggaattata
     4801 tttgtaactg caggaagtct taaactaaga gattttgcta aaaactaata attattaaat
     4861 ataattagta tctattttta tataattgta aaagaatatt tcaacatatt aaattatttt
     4921 atcttatatt tttctcttta gtttcacaca tcaccaagtc taaactttat attatatatt
     4981 aatatttttg acttgtcaag ttatttatgt tataatatta atcattagat aattaaagca
     5041 ttgtacctaa gttgcaatat ctaaaattca atttggaggt ataactagca tatgtataaa
     5101 attggtgaaa gtgtaatgta tccaaaagag ggagcctgct ctgtaaatga tattgtaaca
     5161 aagaaaataa atcatgagat gcaaaaatac tatgaattaa gtgtcatttt taatagtaac
     5221 cttaaaattt cgattccagt tctaaatgca gatagaattg gcatacgtcc agttatggat
     5281 ggaaatgatg ttgataattt tatccaatct attaataaaa cagatggtgt atggattttt
     5341 gatagaaaag aaagacttaa actgtaccaa gataaatttc attcaggaga tgtttttgaa
     5401 attgtaaaat taatcaaaat gcttatgata caagacagtt caaaacaatt gtgtagcaca
     5461 gataaggagt ttttaaataa agctcaaaaa tttgctttat cagaattagc tgctgctcaa
     5521 tgtaaatcct atacaatggt attagaggaa atgaaaaaac atatattaaa ttcaaagaat
     5581 acaaattaaa ccagtttttt aaactggttt tattttttat actaatcatc catgctactt
     5641 tagcttattt taaactgcct aatctaatat taataaagtt atctcaatta gatagatgta
     5701 ttttattcaa ataaaaaaac taagagtaaa tcatattact gatttactct tagctaataa
     5761 aatttaatct tcttcaagtt cttttttctc ttgtaataat acacttaatt catttgcttc
     5821 taaaacttgt gcctctgcaa tatcaagtaa ctcttgttgc cattccacac atttatcttt
     5881 gtaatcaaaa ggtagcgttc tgtcaactat gtacttatca atacagtcct ccgcactttt
     5941 aattatttct tttctttcat caataaattt ttctaattct actaaggctt cttcataagc
     6001 ttttctactc atataaattc cccctttgta caattattct acattaaaat gatttttcct
     6061 cttttttgta taaaatttta tttgtatata acttttccat caatatcttt tatagtttga
     6121 catccagtta atatcataga actacttagc tctgattgaa gtttatttac ataagtttct
     6181 acaccatcag ttgcaccacc aaaagaagca gtcacaaaag gtcttcctat taatactgca
     6241 tctgcaccta aacctatcat ttttagtacg tctaaaccag ttctcactcc accatctgca
     6301 agtattgtaa ctttaccttt aacagcatca gcaatttttg gtaaaacttc acatgctcct
     6361 ggtgtacagt caagcactct tcctccatga tttgatacaa ctattgcatc cacacctgct
     6421 tctactgcca ttaaagcatc ttcaacagtc attatacctt tcaatataaa tggtagttta
     6481 gtagacttta ctaactcctt tatttgttta acatttttag gaagtactgg ttttccatgt
     6541 aatgataaag ttactagacc acaggcatct atatcaactc caactgcaaa tgcaccagct
     6601 tcttcagcta atcttatctt ttttataata ttttcattat cccaaggttt aataaataca
     6661 attccagctc cattatactt tttgactaca tctaaatttt ccattaaaaa agcatctact
     6721 gccgtatcac caaccattgc ataaatacca ctattagcac atcctgcaac tactggttct
     6781 atgtaatctc tttcattaat ttttccaccc atatttaaag tagttcctgt tactggagct
     6841 gcaaatatag gtgcactcat tttctttcca aacatctcta tagatgtatc aggatttgat
     6901 acatcatgta ttactctcat atttactttt actttttcta aactcttgct attttctata
     6961 aaagaagaac cacttccttt acctcccatg ccaggcactt cacctgcaca agcaacccca
     7021 ttacaaacct tacaaacttt acaacttcca tttaaatttt ctctagcact ttttaataat
     7081 tcattatatt ccatctttaa tcccccatta actatagtaa tttatattat atacattata
     7141 tcatattata tttacagtat atttttcaaa aaactttttc tgtggatagg agttatgcca
     7201 tatttttcaa tagcttcata gtgttcttta gttccatatc ctttgtgaga tttaaaccca
     7261 tattctgggt atactctatc atattggtac attatactat ccctagtaac tttagcaagt
     7321 atacttgctg ctgctattga tatagatttt gaatctcctt tcacaattgg attttgagat
     7381 atgtcaatac ctggtattgt tgctgcatca actaataaat aatccggtgc tttttttaaa
     7441 caatttatag cctttttcat tgccatatat gtagcattta atatattaaa ttcatctatt
     7501 tcttcattgt ttactatacc tattccataa tctagtgctt cttcttttat tacttcaaaa
     7561 agttcatctc tttttgcctc actcagtttc ttagagtcat ttacaccttc tattttggta
     7621 tcttttttaa atactacaac agaagctaca acaggtccag caagtggccc tcttcccgcc
     7681 tcatcaattc ctccaatata taaataacct ttatcatagc cttcgttctc aaatatattt
     7741 atagtttcca atctttcttc ttcttttcta atattatcca gcttttttgc cagttttata
     7801 gctagacctt gtacagattt tctttcatca actcttaaca gctctatata ttccatatat
     7861 ttttctactt ctaaagtctc tattatttct ttaatctccc tcacactttt atcctgcata
     7921 ccattccctc atctttcgtt tttaaaaatt taatttttaa atatattaaa attttttaat
     7981 tagagtttaa ttgcatccac tatagccaca ttttaaacat atactacatc cacctgtatg
     8041 tgcaattcct tcacttccac actcagggca cacaaatttt tcatcttctt caaaattttt
     8101 atttattatt tctttggctt tttcatcttt attatctttt tcttttttat tatttttata
     8161 atattctttt ttactatttt cttttttatc ttctttatta ttttcttcct ttttttcatt
     8221 tattaaaatt ccactccttt tacatccatc tctaaatatt gttactcctt taagaccagc
     8281 ctcccatgcc ttaatatata tattgaaaac atcttcaact gtagtttcaa aaggtatatt
     8341 tattgtagat gatatagaag catcaatata cttttgccat actttttgca tgtttattct
     8401 ttcttcatat cctatattca tagcagtaac aaaaaaatct ggtaaacatt cttcatctgt
     8461 aatacctttc ttgtccatat actctttaac aattggagta tatactttat aatacacatc
     8521 ctcatcatgc aagctttcag tttttcttat atatgaaaga ttaaatatag gttctattcc
     8581 accactgata cctatcattg tagatatgga acctgtagga ggtatggtta aaagttgtga
     8641 atttctaagc ccatactttt taaccatttc cttagtgtct tcatttagat tttctataaa
     8701 aaacttagat ttagaaatat tttcatattt atactcacta aatgttccat attctttagc
     8761 aattaatgct gattgcttca cagcttcatt taacatagtc ttaccaatta aatcacatag
     8821 ttctaaagat tcatcagaac catatcttat tcccattttt ataagcatat cagctatacc
     8881 cattacacct aaacctatct gtctatactt atgtacacta tctctttgtt cttgtaatgg
     8941 atgcaagttc aatccctcat ctaagacatc atttaaagct actacagctt ctctaacgca
     9001 agattttaat ttattaatat caaaagtagc atgttcacca aatggttgta ttataaactc
     9061 tgacaagttt attgagccaa gtagacaact tcctccactt ggaagtggtt cttctgcaca
     9121 aggatttact cctgaaaagc aaaagttctt atcttcactc ataaaattat atgtttttat
     9181 tttatcccaa aacaatatac ctggttctgc ataatcccag ttatttttca caaatttatt
     9241 aaataaatca taagcattta ctatcttaac tattttttct ccagtagttt caacttcaaa
     9301 agtacaatta tataactttt tttctcttac agcttgcata aattcatctg ttactcttac
     9361 agaaatattt gctttagtaa ccttctccaa atctgttttt atatctataa agtcttctat
     9421 ctctggatga tttacatcca tagaaatcat aagagctcct cttctccctc tttgaccaat
     9481 taagccagtt gtcatactgt aaagttccat aaaagagact gctccagtcg tatgttttgc
     9541 agcattgtta actttcgctc cttttggtct tagctttgaa atatcaattc caactcctcc
     9601 accataacta aaagttctag caagtttttt tgcagtatca aatatatctt ctatgctatc
     9661 ttttggtggt tctattacat aacaattaga atatgttact ttttttccct ctttctgtaa
     9721 tcctctattt gctaaaattc ttccagcaaa tagaaatctt ttttgtctta ttagttttga
     9781 tattcttgaa ctattatttg aaattctctt tatccattca tcaaaactct caccattatg
     9841 tctatacttg ttcttccata ttgatttttg taattcatca aactcccaag ctttagtcct
     9901 tatttcagag cgtctttctc tatataatat gtagtgtttt gctatatctt ttctatttga
     9961 ttccataagt agtacttcaa ctatatcttg tatttcttct acggataagc atttttccat
    10021 agaattatag tcattagcta tttttcttga tatatcatat gccaactctc tatctacgcc
    10081 accttcacta tttatactag ctttatagac agcatcttca atcttacttt tactaaaaag
    10141 ttcttttgta ccatctcttt ttattatgta cttcattttt aaacctccaa taaattagta
    10201 ttaaattaat acacacatta actataactc taaacaaact ttttatatat ttgtatattt
    10261 tcatattaat tatctaacat aatatatatt atactatttt atattcaata taacactcct
    10321 tatagttttc ttaacttgta aaatatcttc ctaaaaaact caaaaataat ataaaaaagt
    10381 atataaaagt caaattatac tcttatatac tttatttttt tatatttttt tcattttaac
    10441 tttgattaat taataagtat ttagaaaata aattctttat tttttcttta caaaaatatg
    10501 tattagtgtt tttatattaa tactaaacac ttataatata taatttaatt ttatttataa
    10561 ttataaatga atctttttta ttttattatc tttataaatc atact
//
LOCUS       DAEMHZ010000138        10387 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000138 DAEMHZ010000000
VERSION     DAEMHZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.139"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(350..847)
                     /locus_tag="KPK72_003158"
     CDS             complement(350..847)
                     /locus_tag="KPK72_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG2490351.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            complement(953..1315)
                     /locus_tag="KPK72_003159"
     CDS             complement(953..1315)
                     /locus_tag="KPK72_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490352.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            complement(1333..1947)
                     /locus_tag="KPK72_003160"
     CDS             complement(1333..1947)
                     /locus_tag="KPK72_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2490353.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            2542..4677
                     /gene="rnr"
                     /locus_tag="KPK72_003161"
     CDS             2542..4677
                     /gene="rnr"
                     /locus_tag="KPK72_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG2490354.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            5469..5960
                     /locus_tag="KPK72_003162"
     CDS             5469..5960
                     /locus_tag="KPK72_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG2490355.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDNFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
     gene            6489..7361
                     /locus_tag="KPK72_003163"
     CDS             6489..7361
                     /locus_tag="KPK72_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490356.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            7358..8647
                     /locus_tag="KPK72_003164"
     CDS             7358..8647
                     /locus_tag="KPK72_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490357.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDNILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            8577..9221
                     /locus_tag="KPK72_003165"
     CDS             8577..9221
                     /locus_tag="KPK72_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490358.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(9488..9952)
                     /locus_tag="KPK72_003166"
     CDS             complement(9488..9952)
                     /locus_tag="KPK72_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490359.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         4037 a         1202 c         1522 g         3626 t
ORIGIN      
        1 ttatataaat tattaaattt tatataagct attgaatttt atgcagatta ttgtatttca
       61 tttagattat aaaactttat gtagagttaa tgaatttaac atatattatt agttatatta
      121 gtgcttttat tttaatgagt ctaataataa ttctaataga aactgaaata gataagtaag
      181 ttttaagcta aaatcaattt atataaattg ataataatct aatataaatt aaaataccag
      241 tcaactaaaa tatatattaa gataaagtgc agaaaatatt ctaataacaa ttaagctaat
      301 catcaattaa atgtagcatt taattaatga ttagctatag tcaacctaat taattagaat
      361 aacttccttt tagtgtaaat gtatctaaaa catgcccttc aatagctttg aaaaattcat
      421 tcatatagaa tcctttttca aggtcaagga tagtatcaag tgcaaataca tgtatttcat
      481 agacatgagg tctgtctgga ggagccattc caccatatac actggcctct aatctgtcaa
      541 gacctcctag tttaccatgc caactagtag tcccttgtac aaaatcagta gctgttacac
      601 tttcattttc atttaactca tcttttgtta tatttacagc taaccaatgt atccatgcaa
      661 acccacatac aggaacagag tctttatctt ctaaaaatac agcaaaggat actgtattct
      721 ttggagcatc ttcaaacttt agaggtagtg aataggtagg cataccacct tcattgaact
      781 gagttcctct tttaccgtat ttatcttcta taacaccatt tactatacct gtactagtta
      841 ctttcataat aatttacctc ctattataat aaattaatag aatatatcta taatttaatt
      901 atataccagt tgaaaataat ggtaaattac ttactttttt gtgggtatct atttatttta
      961 aaacaccttt ttccttaaga atatcttcca taccattatc caacataaag ctaattccta
     1021 tatcttgcat tatagccaaa gcatccaata tttgtttccc tcgaataggt gtaagtgagt
     1081 attcaacttt aagtgggtaa ccttcatatg tctttttatc tacaagacca aattcaatta
     1141 attctttaag ttgttcaagt aacatctttt gattaatacc attgatttcc ttttcaagtt
     1201 ttgataagga agttggtcct atgcgtaatc tccataatat aatagatttc catttgcctt
     1261 ttatcatatc atgtgttagt tctaatgggc aagtatactc atcacgaagt ttcaaataag
     1321 ttcacctcca ttttactttg ccaagataca ttttgaagca agcttatata caaactcaac
     1381 ttttttattt gatatatccc atgaatctaa ttcttttttt actaaatctg gatatttttt
     1441 acttatatct ctaagagcat tacctacaga ctttctcaag tattcgctat catcttcttt
     1501 taaactagaa agtagtgata tagcaacgtc aggattagat ttaaaatagt ctctactagt
     1561 ccaaattcga agaccttctg tcacagccct ttttatgttt ggactatcag aattaagcca
     1621 ttcttttata acaggaagag ccttttcata tcctacttca ctgcaataat aatcaaatga
     1681 tttagctagt atttcttgta ctctccagtt ttcatcttta ctcacattat cttttaaaaa
     1741 taacaaagct tcagaaatag ttactgatat aaaaccaagt ataaacacag ccaattctct
     1801 tacttgataa tattctgacc tatataagta gattgataga taataacact tttttgagtc
     1861 attatttttt acaatttttt tagcaatgtt ttcaatttct ttgaatccat tttctatagt
     1921 aataattttt gcaattactt cttccaattt tatctcctcc aataaataga ttagtttaaa
     1981 ttttatgtat ttatttgtaa aaaagtttgt ttaatttaga caaaataaag acttgtcaaa
     2041 aaacgtttgc aagtctttag tactagcata aataaaaaat aataaaaaat caacctcatt
     2101 taacatataa atttactttt attataagtt ttatattata aagttgtatt taaaaaatta
     2161 attgagttat ataaatttta catgatttga gtatactaat agaataaatt attatatagt
     2221 aatacttata tgagatgaat attttaccaa tcctatttga tggtgtataa ttaggtgata
     2281 aagagtaatt taagcaatta tgataaagaa taacttaagt aattataatg atatttacaa
     2341 ataatttata aaacatcata atttgtttga agttagtatg acaaagctac attaaatcaa
     2401 aaatataaat aagtaatttt aacttgtaat aataataagg aaatatagaa aattatgtga
     2461 taagtaaaaa atgttttgtc tgaagaaata taaaagacaa tataaataat aatataatga
     2521 aaagtaaaac aggagggaaa aatgatacca ggacttaaag aaagtttgct aggattaata
     2581 aatgaaagtg catataatcc tctaaaaaaa gaagaattag cagaaatatt tgatatacat
     2641 tcatcagaaa tgccaatgtt ttataatttt ttaaatgaat tagaagaaga tggttatatc
     2701 tgctttacta aaaaaggaaa aatagtatct cccaatcaaa tgggatactt tgttggaaaa
     2761 tttgtatcac acaaaaaggg ttttggattt gtagaatctg atgttgaata tacacaggat
     2821 ttatttatct caagtgacaa tataaatggt gctatgcata atgatagagt tatggcagaa
     2881 atagtagtac ctgcaactga agaaaagaga gcagaaggaa gaatagtaaa agtaataaag
     2941 agagaaataa ctagaatagt aggtactttc caatcaagta aaacttttgg atttgtaaca
     3001 ccagacaaca agaaatttac taaagatata tatataccta aaaaacattt tagtggagca
     3061 gtagataatg ataaggtagt atgtgaaatt acagtatggc cacaagagga tagaaaacca
     3121 gaaggcaaaa ttatagagat actaggtcaa aaaggtgaaa gaggcgtaga aatagattct
     3181 ataataagag aacatggtct tccagaagaa tttccaaaga aagtattgca agaagcagaa
     3241 gctgtagctg ttgagattcc tcaagaagag ataaaaagaa gaagagattt aagagattta
     3301 aacatattta ctatagatgg agatgacgca aaagacttag atgatgccat atcaatagaa
     3361 gtattaccta atggaaactt caaactagga gttcacatag ctgatgttac acattatgta
     3421 agagagaaaa ataaacttga caaggaagcc ttaaaaagag ctacatcagt ttatttagta
     3481 gataaagtaa ttccaatgtt gccaaaaact ctttcaaatg gagtatgtag cttaaatcca
     3541 tttgaagata agttaactct ttctatattt atggaaatag accataaagg aaatgtggtt
     3601 aaacatgaaa tatgtgaatc tgtaataaat tcaaaagcta gaatgactta tacagaagta
     3661 tctgatatat tagaaaagga tgatgaaaaa cttaaaaaga cttttgaaca tgttgttgaa
     3721 gattttaaaa atgctgaaat tttagctagg attcttatgt caagaaggga aaaaagagga
     3781 gctatagact tcaatttccc agaggcaaag ataattctaa atggaaaagg cgaagttgta
     3841 gatataaagc catatgaaag aagaatatct aataagataa tagaagaatt tatgcttata
     3901 agtaatgaga caattgccga acatttttac tggatgggaa taccgtttgt gtatagaata
     3961 catgagacac catctttgga aaaaatggaa gaattgagta agtttatatc tacatttgga
     4021 tatataataa aaggtgataa agaggaagta catccaaaag cattacaagg tataatagag
     4081 aaaataaagg gcaaaaaaga agaaggagct atcagtacta taatgcttcg ttctttaaaa
     4141 caagctaagt actcaccaga atgtagtgga cattttggat tagcagctaa attttactgt
     4201 cactttactt caccaataag aagatatcct gaccttcaaa ttcatagaat aataaaagaa
     4261 tctttaaata ataaaataag tggaaaaaga caagaacagc ttactactat agtggattat
     4321 gcatctgttc aatcatccga gaaagaaaga aaggcagagt tagctgaaag agatgtacat
     4381 gatttctata aggctcttta tatgaaggat aaagttggtc aagaatttga gggtgttgta
     4441 tctagcgtaa cttcatttgg aatatttgtt gagttagata atactataga aggattgata
     4501 agacttgcaa atatgaatga tgattactat atttatgatg agagttctta tagtgtttta
     4561 ggagaaagaa ctaagaagtc atataagata ggagaccttg ttaagataaa ggttgaaagt
     4621 gttaatgtag attttaaaga aattgacttt gagatagttg aaaaaataga ggagtagata
     4681 aaacagctaa aataagggat aacaagttgt agtatttatg atatgtataa atatatagtg
     4741 cacaatactc tataaaataa atgataaaaa taggtaattg agaaatatct aatggaaata
     4801 gttaactgaa agataaataa ttaagaaata tttattgaaa gtagctaatt gaaaaataag
     4861 taattaagag atatttaatg taaacagata atttaaaaat acctaattaa gaatagatat
     4921 taaaaaatat aatttcaata taaaaataag tgtatcattt aaaatttaag atacacttat
     4981 tttttattta agataacttt tcttttttag aatttataac aataggttaa tatatttata
     5041 attaatgtaa aaattattca tatctattaa atcaaattct tacacatata attattggta
     5101 tgcttattaa cactccagaa actcttatag tttaaaattt aaatttattg ttatttatta
     5161 tttaatttga gaacatgatt tattttttat ttgaagctaa taaatagagc atgattctac
     5221 agtataattt cttaaatttt tttaaaaagt aatagttctt atttatagtc aatattgcct
     5281 atagaacttc tgattcattt aatttttagg aaaaaataat aatacttacg ctataaacac
     5341 agaattttag ttgataatat aggtatttaa atttaagatg aaataatact tttctcaata
     5401 tattttattg taactaagtt taattataag ttacaattaa aaaaacgcta cttaggagga
     5461 atgtaattat ggggatgata ggatgttata ctaaaataag tgaagagaat gtttttaaat
     5521 tacagcaagc agaagaaaat ttacaagatt ttgtttttga agatgccaac gaaaatagta
     5581 ctatcaatat tgataaagca tggcatgcca ttcactttac tttgacaggt tgtccttttg
     5641 gtggagatga tgataatata tttagcaaat tagtactaag tggaaacata cttatggaaa
     5701 ttgatggaga atttcctgtc atgttgatta cagcaaatga tgtaaaaaaa ctatcaaaag
     5761 caatgaacag tttggaagaa caagcattta gaaaaagatt taatattaat gagatgttag
     5821 aaaataatat ttatcctgtt atgaatgatg agagtgaaga taattttttt gaatatgtat
     5881 gggcaaatct aattgagctt aaaaagttta tacaagaagc atctgatgat ggactagctg
     5941 taatattttt tataatgtga taattactaa aaattgtgaa tattagtgtc atcacatatc
     6001 ttagatatga agccttttga ttgtatagaa gaaaatacat attatattag ccttttaaaa
     6061 tatataagtt aaatttaaat ctgtaggtaa atttgtctta tgtctttata aatatttata
     6121 ttagcatata taaattagtt ttaaattttt gcaatatata gttgtagata tttttattta
     6181 tcttaaaagg ctaaataccc tatcctatta tatccagtaa ttcttttgga tacttatcac
     6241 atttttcatt atatataaaa atagactcac aaataaaata aatatatatt aaattataac
     6301 ttcataaact taaaaaattt cagaaacaaa gatactttaa tttaattaaa aacagaaaaa
     6361 ttaaaatctt tagaaaaaat ttagaaattt ataaataata tcttaagttt tagaatttat
     6421 tatgaaaata cagtaaaaat ttataaaaat aaaattctat aaaattgttt ctgggggtgt
     6481 atttatatat ggaaatgaaa atagcagtat ccaatcttag taaaagattt gggaaaaaac
     6541 aagctcttga taatataaat cttgaaatcg acagtggtat gtttggtctt ttaggaagaa
     6601 atggagcagg aaaaaccaca tttatgagaa ttttagccac tttattatca aaaacagaag
     6661 gttcaataca tatgtgtgga gttcctattg agaatactaa ggaaattaga aatatggttg
     6721 gttatttgcc acaagacttt tcaatgtatc ctaacatgac agtatataaa gcaatggatt
     6781 atttagcagt tttatcggat ataccactaa gaaaaagaag aaaaattata gcaaagttat
     6841 taagcaaagt aaaccttact aattatcaaa atgtgaaagt aaaatcttta tctggaggta
     6901 tgaatagaag gcttgggata gcacaagcat taattcataa tcccagagtt ttaatagtag
     6961 atgagccgac agcaggtctt gacccagaag aacgtataag atttagaaat ttactatgcg
     7021 aaattgcaaa tgatagaatc gttattttat ctacacatat tgttggagat atagaagcta
     7081 catgtgaaaa cgtagctata ttggatagtg gaaaagtcat atacaatggc tcaacagaag
     7141 cattagcaaa actagctgaa gggaaagtat atagtattga agtagataaa aaagacattg
     7201 aaaatataaa aagtagattt atagttatag gaatgttgac tcatggtgga aaggctatac
     7261 ttaggattat atcagatgat aaaccttttg agacagcagt aaattgtaat ccaactattg
     7321 aagatggcta tatgcttata atgggaggag ataatatatg aatctattta taaaagaatg
     7381 taaaaatata ttaaagagta tggtatatta tatttttatt gtagttttag tttttttcta
     7441 tatttctcaa cttggaagtt cagtttcaaa tgatgtaagt aaaaatattc ctaatgatgg
     7501 aaatccatta gttgctccac aaaaagtaga gaatattgag gaatattatt ctgtacacaa
     7561 tcaatatcca tatggtcaaa aagaatctat gaaaacagaa aaggtcattc caaatgcaac
     7621 tataaaccta attagagaat atcaaaaaaa cacatataca acgtatccaa caggggtagt
     7681 taagtatata aaattagacc aaaataaaac gaagaccata gaaaaatata tagaagaaat
     7741 aactggtaaa agtattagcg aaataaattc actttggaaa actgatgaaa gtataaaaat
     7801 agattattct aaagtatctc ttgaaagatt tgaagaaata atgcaaagag tagacaacat
     7861 tttaggagga gggtctgcat atgcttttga caagttacaa agatttggag tagttccagt
     7921 tacctatgat gaagctatga agaattacaa ctatacaata caaaaagata aagttagtaa
     7981 tgggtatgca agaatttttt gcgattatat aggtatagtt ttaagtatat ttccagtatt
     8041 tgtggcagtt tttatgtcac ttaaagataa gcgttcaaga atgagagaga ttatttattc
     8101 aaaacaagta tcttcatgca aaatagtttt atcaagatat tttgcacttg tctttatgat
     8161 gatattgcct gtattaattt taggtatgaa agatttactt cctttgattt tctttgctaa
     8221 agataaaggt atatctattg ataacttagc atttataaaa tatacattat ggtggatact
     8281 accaactctt atgatagtta cagctgtagg aatgagtatt actatattaa cagatacacc
     8341 agtagcaatt attgtgcaat taatttggtg gctttacagt ataaattcta caggactcaa
     8401 aggtgattat tcaacaatgg gactaatgat aagacataat agtgtattaa atggggatat
     8461 tattatacaa aacttcaatc aaattatgat taatagaatt gtgataagct tgggagcatt
     8521 ggtgcttgtt ttgttagcta tattcttata tgagcaaaaa agaaggggga atcttgatgt
     8581 ttgggcaaag ttttcaaaac tgtttagttc taataaaaga aaacttcaaa ttaagcactt
     8641 taactaattt aatatgttca attatattgc tatgtatgat tcctctaata caaggagtag
     8701 ctaatttaga cagaatttca acagctatat gtttagaaaa ttatgttgca ataataggga
     8761 tagtaatgtt ggtacctgta ttttcgctag agcaatcaaa agaaattgat gagatagtat
     8821 cttcaaaagc attatctcaa gttattgtac aattaattag gatactgatg tcaattatag
     8881 gtatagtggt attaatcata ggttttgtaa atgttttaat atatcttgga tgtaattttc
     8941 cagtagttga gtatattatt ggaagtttcg catcagctat gtttatagga agtttaggaa
     9001 tgtttttctc aaatttattt aataatacaa tcataggtta tatgatatct ataggatatt
     9061 taattttaaa tatgatgact aaaggcaagt atgttggaaa attttttata atgtctatga
     9121 gtcaaggtag ttttgatgag aaatattggc tgatacttgg aagtgttgtg ttgataattg
     9181 catctctctt aataaaacca gtaaaaagga agattagata ataaaatgag agcttagata
     9241 aaataaaggt aatacaaata aaaggtacat ttatgaaata taaatctatt tttatataca
     9301 caaacctact tacaatctct ttatgtatgt agatagattt atatatccgt agtataaaat
     9361 atatatgtat accaaatttt atcaaaagct ctcaaattta tatttcaaag acacttattg
     9421 ctatgaccta cacatatgta tttttttatt ttaattgttg tattaacaat aagactattg
     9481 ttataaacta ttgatacctc ataacaaaaa attctccatc tcctaaatgc gttttctgta
     9541 ccttagtctc aactatttta aatccttgag actcataaca ttttacagct ctcatgttcc
     9601 aggttcttac atttaataca attggtttag tactatattt atttctacat tcttgtaatc
     9661 ctaggctaat tatttcttta ccaattccca ttccacaata attcggtttc ataccaatac
     9721 caaagaatac agagcttcct tcatctaata agtttataaa acctatcaat tctttattgt
     9781 catttataaa acttataaaa ttttttcttt tatcttcttt tgctagtgaa aaattcttct
     9841 ttttaatttc ctcccatgga ggtagattgt atattgaata tttaccttca tatttccaat
     9901 tagtaacttc aatagcatgt tcttcagtaa atttaacttt atttaaattc atatgtcctc
     9961 ctattaaaat atttaaggta attgatggta ttttaaagtt ttagaaaata agtaaaacta
    10021 tttatattta tccataaaat ctttaaaaat tttactaaca attcttgtac cctgtacttt
    10081 ttattggatt cacagaagac tataaactaa ttttgaatta ccttctaatt caaaaacatc
    10141 acgatatata tataatttct tagcttgatt aaattctgtt aaatccgttt cttatagttg
    10201 tataatcaac atcaatatct ttatgtgtat tagttaattt attggtttta ggaacttatt
    10261 attaatatat tgtatttgct ccaataatcc aagtagtagg taaattatag gcaaattatc
    10321 aaatgtatat ctatccatgc ttaaacttta gagatacaat aactactaaa ttagtattct
    10381 agtagtt
//
LOCUS       DAEMHZ010000139        10385 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000139 DAEMHZ010000000
VERSION     DAEMHZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.140"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            17..931
                     /locus_tag="KPK72_003167"
     CDS             17..931
                     /locus_tag="KPK72_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG2490360.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            1321..1968
                     /locus_tag="KPK72_003168"
     CDS             1321..1968
                     /locus_tag="KPK72_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2490361.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(2135..2704)
                     /locus_tag="KPK72_003169"
     CDS             complement(2135..2704)
                     /locus_tag="KPK72_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2490362.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(2709..3608)
                     /locus_tag="KPK72_003170"
     CDS             complement(2709..3608)
                     /locus_tag="KPK72_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2490363.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(3619..3768)
                     /locus_tag="KPK72_003171"
     CDS             complement(3619..3768)
                     /locus_tag="KPK72_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490364.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(3761..4345)
                     /locus_tag="KPK72_003172"
     CDS             complement(3761..4345)
                     /locus_tag="KPK72_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG2490365.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            4734..6059
                     /gene="cdeA"
                     /locus_tag="KPK72_003173"
     CDS             4734..6059
                     /gene="cdeA"
                     /locus_tag="KPK72_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG2490366.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFIFLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISISLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATSIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSQDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(6154..6948)
                     /locus_tag="KPK72_003174"
     CDS             complement(6154..6948)
                     /locus_tag="KPK72_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490367.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(6949..7824)
                     /locus_tag="KPK72_003175"
     CDS             complement(6949..7824)
                     /locus_tag="KPK72_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490368.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(7828..8196)
                     /locus_tag="KPK72_003176"
     CDS             complement(7828..8196)
                     /locus_tag="KPK72_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2490369.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEVRKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(8378..9037)
                     /gene="deoC"
                     /locus_tag="KPK72_003177"
     CDS             complement(8378..9037)
                     /gene="deoC"
                     /locus_tag="KPK72_003177"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG2490370.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(9073..10260)
                     /gene="murJ"
                     /locus_tag="KPK72_003178"
     CDS             complement(9073..10260)
                     /gene="murJ"
                     /locus_tag="KPK72_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2490371.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
BASE COUNT         3618 a         1620 c         1181 g         3966 t
ORIGIN      
        1 ctaaagggag gaaattatga tagataatca aaaatatgtt attttatcac tagagttaca
       61 tttatttttt tcaagaatta tgaaagagca tgctcttttt ttagaagcag gattcacaaa
      121 taaaaattat aatcttgcta tggaagctga ccactataaa aagcaatttg aagatttatt
      181 atcatacact gttagtgcta gtaatggtat aattagacct gatatattat attcagaaga
      241 gcttgtaact actctcacat cagttgcaga acaaaaaaca gaggagttta cagggataga
      301 aataaacaaa aacatcacta caagagaatt aaatctacaa agtggtgtaa acccacaagt
      361 tggtcaagac ttagtgaact atgtagctca acttaactct gatgcaataa gattacttga
      421 tgggcttatt aattttaaag aaagagtctt agatggtgta ctatcatgta ctatatttac
      481 atcaaactac cctctactcc ttgaacatat aatacatgaa gcaaatttat atcgttctta
      541 tgtagttgac cttgaaaata aaatagatat tgagtcaaaa aacgctaaag aaatagaatt
      601 attctgggac catattatga tggaacatgc tctgtttatg agaggattac tagacccatc
      661 agaaggtgaa ctaataaata cttcaaatga ttttgctata aaatttaatg aattaattga
      721 aaaaacaaac gaaatgactg attctaatat caagaacatt acagaagaaa ctctaaatga
      781 aactgttgag tttaaagatt ttaaagaagc aggagcatca ggaatagaac agtgtaagat
      841 aaaatctata atattaccac ttttagcaga ccatgtttta agagaggcaa atcattatat
      901 tagaatattg gagagttata aaaacatgta attaaaaatt acttttaaaa taatctttgc
      961 atatttagat aaaataaagg tacagttaac tgtaccttta ttttttataa acttataaaa
     1021 tttaaacaag ttattcataa aataaaataa taatcatcta taacccacaa ctttatttta
     1081 tattaaataa aaagtattag tgattatgtt ttataggtat ttctttattc agttatttat
     1141 taaaaactaa tagtaaatct tatactaatc gcatatttat gttataacta tattgtaact
     1201 attttcgtaa tcttaggaaa aggttactta taaaattaac cttatacaat aatatatcgt
     1261 ataatcttaa ttagcataat agttacaata ttaaaattta aatatgggag tgattttagt
     1321 atgataagac aaacaccatt agagaaacga tatgacaagc taggtcctca tattgtaaca
     1381 gcactcaaaa aacgttattt tgatgcttat tactgtaaaa ctagaaatga agctttgcaa
     1441 caaattttag atttaattcc tcaaaatcat gtagtatcat ggggaggatc tgaaacactt
     1501 aaagaaatgg gcgtacaaac tgctgttaga gagaaaggat ttaaagttat tgatagagat
     1561 ttagcaaaaa ctccagaaga aagaactgaa attatgcgtc aagcacttct atgcgatact
     1621 tttttgatga gtagcaatgc aattagtgaa gatggacaac tctttaatat tgatggaaat
     1681 ggcaatcgtg tagctgcaat gatatttggt ccaaaaaatg ttatagttgt agctggtatg
     1741 aataaaattg taaaaaacat tgatgatgca atacaaagaa ctagaacaat tgcatctcca
     1801 gcagttgttc agcgttttca agatgtcaat acaccatgct atattaatgg aagttgtata
     1861 gattgcacaa gtcctgaaag catttgtgca tacatggtta ctacaagaat cagtcgtcca
     1921 gcaaaaaaaa taaaggtaat attggtagga gaaaatcttg gtctataaaa tatacataca
     1981 tgtaaatagc tctataaata ataaaatcat atgaaataac ttatttctaa aagtggtaga
     2041 ataaaagctt ctacaagact ttcattctac cacttttata ttcttttaat ttgtgtttct
     2101 atattattaa ttgctccgat tttcatatat attactaact atttttattt tcataatttt
     2161 tttgttcttc atcaagctgt ttataataat ctttattata gtcagctaga taatctatac
     2221 cacctcctgt aatcacattt aataatttca ttgcagattc ttgattaact ttacaaacat
     2281 aattatcacc agatttaaca agctctaatg taatattaaa ttcttttttc tttgcagaat
     2341 caatatcttt catagttgga tttggcatca tttctcctag tttaggagga ttctttttta
     2401 aaatagaaac catatcagta ttgttaattt ttaaatctac agtcacttta tccttattat
     2461 tctttatatc tgaaatttcc caagtcatat ttttagaata cattttaaat ccttctttat
     2521 cttcgtatat aagattacct aaccctgatt ttgggaaata cttatcaaca gtatctgcat
     2581 cttgtgtttt tattgaatta aacatactat ccacttcatt ttttatctta ttatcattag
     2641 tacaaccagc taacaccagt actgatattg caaacattat aatacatata ataaatttct
     2701 tcattaaatt atatccccct atactttttt gcacaaatat tattctcttt tttatctttt
     2761 gaaaaagttg tagtaattgc attttgtgga caaactttta tacattcacc acacctaata
     2821 cagtctggac tatttacatc tttatatatt ggtatatcaa gcttacattt tgtttgacat
     2881 gctgtacaat ttgtacattt atctttatca attttaaatc tatatattga aataggatta
     2941 aaaaaaccat agattgctcc taaagggcat atatacttgc aaaatggcct ataagtaatg
     3001 attgacatca ataatatcaa tagcaaaata cttaacttcc agctaaataa aaatcctatt
     3061 gattctctta aattctcatt tcctaaaata agtggtattc ctcccaaaag tgttcctgaa
     3121 gggcaaatcc atttacaaaa ccaaggattt ccaccccctg ctacatttac aatagcaact
     3181 ggcaaaataa ttacaaacat tattaagact atatacttta gatattttag atatttatca
     3241 ccataaactt tttttatctt tactggaaag ggtactttat gtagtaaatc ttgtacaagt
     3301 ccaaagggac aaagccaacc acaaacaaat ctgccaaaaa aagcaccaaa tgccattaaa
     3361 aaaccaacaa cataaaatga gaatttatac tctctgcttg ataaaactgc ttgtaatgaa
     3421 cctattggac atgctccaag tgcacctgga catgaataac aattcaatcc aggtacacat
     3481 aattttttac tttctcctcc atatatagtt ccttctaaaa atccatttac atatccattt
     3541 gttaatgctg taaatgcaac ttgagtaata agtcttatat tattatttac tttagataat
     3601 aacttcattc ctcttctcct aaccaatacc aatacactca agacaaatca taattgcttt
     3661 agttaatact gtatttgttt ctccacgata tgtacctaat gcaataaata taatactaat
     3721 tgcaataata gaaatgcctt ttttattatc tttaataaag ttactcattg gcttttacca
     3781 tttttagtct ttcatctata atttttttcc aatcttcctt actatttgca cctacaacct
     3841 tctcacccac aaagtttcct ttcttgtcta caaaataagt tgttggaaac tccataactc
     3901 ttttaaaatc agttttttta agttcttcag atacagtcaa ttgttggtaa gttgcatctg
     3961 actttttcat aatgtcttta actaaatttt tctcttcatt tgaaagacca gttctcatat
     4021 cagttttatc aacttctaca actaatccct ttatagctac attggaatta ttactctcat
     4081 attcttttga taatgcttca agctctggca tttcacccac acatggtcca caccaagtag
     4141 tccagacatt aataagagta agttctttat ttgaaaatat ctcttttgtt accttatttc
     4201 catcaacatc ctccgtatct aaattactaa accattcact attattcttg tttttttcta
     4261 ctggcttaga ttcttttttg ctacatccag aaatcatacc taaaacacaa atacatccta
     4321 aaactactat gaaaaacctt ttcatatctc acactcctag aatttatata gtatataaaa
     4381 tcgctgcttg aaataaaaat aacttatctt ctatattaaa gtaattatat ttaataaata
     4441 agagctaatt taaaaaaaga tatacagtta aaatctattt cttttcagac aaaatcttat
     4501 atatatataa tagcttgtaa atcttaagat tatatacata tatttctaaa gattttctta
     4561 agaaacagtt agattttact aatataagtt tattaattag cattatacta atttatctta
     4621 atatgatttt ttgttgattt taagctgttt atcgtataaa taaaattttt cttgactata
     4681 tagttactat atagcttatt atatatagag agtaataaaa ggaagtaaaa tttatggaaa
     4741 atttatttac aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta
     4801 tatccatgat atttatattt ttgtacacaa taatagatgg catctttgta tcgacattag
     4861 ttggttctga tgctcttgct agtataaata ttgtactacc tataattaac cttgtttgtg
     4921 gatttggaat aatgatggca actggtggag gtgctatcgt ttctatacgt atgggtgaaa
     4981 atagacagga tgaagccaac tctacatttt cttttatagt tttgttttca ttgattgttg
     5041 ggattttatt cacagtaatc tcatatttct ttatcaaaga aatatctatt tcgcttggtg
     5101 caacagataa gttattacca tattgtataa cttatggtaa agttatgatt ttatgtacac
     5161 cattttatat tttaaaattt atatttgagt actttgcaag aactgatgga aattctaaat
     5221 ttagtttatt tctatcagtc attggtggtg taacaaatat aatattggat tatgtattta
     5281 ttaaatattt tggaatgggt cttttaggag ctgcagttgc aacttctata ggtattattt
     5341 taacttgtgt tttaggtatt atttacttct tatctaataa atctacacta aaattaagaa
     5401 aaccaaaaac cgattttaga cttataagag atactatgat aaacggttct tctgaaatgg
     5461 ttacagaatt atctacagga attacaacat tcttatttaa tgtagtagct ttaaaattag
     5521 caggagaaaa tggacttgct gctcttacta tagtattgta tgctcatttt ttaatgacat
     5581 cagtctatct aggattcgct gctggagtgt ctccattaat aagctataat tttggtgctg
     5641 aaaacagtga taaattaaaa gaaacattta aacattctct aaaatttata tttgtttctt
     5701 ctcttttagt gtttattatt gctttagtat ttgcaccatt tattgttagg gtctttgtaa
     5761 gtcaagataa cacagtattt aaactagcct tacaaggatt aaaaatattt gcatttgctt
     5821 ttttgtttgt tggtataaat atatttgcat caggattttt tacagcattt cacaatggaa
     5881 aaatttcagc tattatatct tttagtcgtg cctttgtttt tataatcata ggaatcataa
     5941 ttcttcctcc tatgttaaac atgactggat tatggcttac agttccattt gctgaggtta
     6001 taaccatatt tatatctatt ctatttataa aaaaatataa atgtagatat aagtattaaa
     6061 aattaatact taaaatcaac atttattaaa actaataaac ctgctttaaa atacaaattt
     6121 acaaatttga tttttaagca ggttttattt tagttataat aagtcatttg catactcttt
     6181 aattggtgct tttctaagta ataaaattat aaatattatg caaataacta taattcctac
     6241 ttttgttcca aataaaataa aaggattctg aaatgaataa ataatttttt ctaaatacaa
     6301 tagcatttta tgcatatttt tagttgcaaa attaactata taaattatca ttaatatcgg
     6361 taatcctatc aatatagtaa tactagtaac ttttctaact ctatatgtaa aagctccaag
     6421 taacactcct ataaaaccta tacatatttc atttatacat atttgtacaa aataatttaa
     6481 ataagttagt ggcatacttg atgttgaagg atctatcagt gctgatatag atgtccaaat
     6541 catatctctc tttagtaaaa ctgatagttc tatactataa cctgttataa tttctataaa
     6601 taacttcgat aaaaaaatca aaactatacc taaaatagaa atcacaaagc ttaaaataat
     6661 taaatctatg acaatagctt ttatacaact ttttctatct gctcttatac ttaaagctcc
     6721 tgaaaagtct ttatttgcta tatttacccc atacacaaat agcagtatag ttatatatgt
     6781 taaaatagga ttcacaatcg tggataaatt agttttagat gcttctccaa aaatcttgag
     6841 gataaacact ggcaaaatat tcataacaat tcccacaatc ataaatataa ttatatacaa
     6901 tttagtattt ttattaaaaa attttatata tggctttaat tcattcaatt acaattcctc
     6961 cttcttagtc atgttaacaa acatatcttg tagccctatg tatcctacat caatatttga
     7021 atttttaagt attttttcat cattttcact aaaatcacca tagtaaaaat aagcaactgc
     7081 atttccaaat gcctttttag gttttatatt ttttatacat tctaattttt ctaactcttc
     7141 tttatttcca gtaatataat gagctttttg ttttatgaca tcaatttctt catctataat
     7201 aatttttccc tcatttaata ttattacatg ctccagtaaa tcatctattt catcaatcaa
     7261 atgagttgat attattattg tccttggatt ttttatataa ctatctaaaa ttatgttata
     7321 aaactcttgt ctatttactg catccaaacc aattgttggc tcatcaaata tggtgatagc
     7381 tgaattagaa caaataccta ttatatttga aagtatagtt ttcatacctc ttgacagttg
     7441 tttatacttt tttttagtat ttaaatcaaa atatttgcat aattcttctt ctaagccttt
     7501 atcataactt ttataaaaac tagaagaaaa ttcaaatatc tgacctactt taaaatctgg
     7561 actataaaat tctttttccc tgacgataca aacttcttcc aaaactttta tatcttcttt
     7621 tgggttcttt cctaatattt ctatctcacc ttcattttga attaactgat ttaccattat
     7681 atttaataat gttgtttttc cagctccatt cttacctaat aaaccatata tcttattttt
     7741 ttcaaagttt agagacatat tattaaaaat attttgcttc ttaaaactgt gagataaatt
     7801 ttctatctta atagaactac tcatatatta atcctccttg aaattaatta taatatcaac
     7861 caattcttct ctatttattt ctaatttgtt agcttcttga agtaggtttt ttacaaaatt
     7921 atgtttaaaa ttttcttttc taacttcttt tataatagtt tttgcaccat ctgatacaaa
     7981 catccctatc cccctcttct tatataaaat atttttatca accaatatat ttataccttt
     8041 taatactgta gctggattaa ttttataaag ctttgaaagc tctgttgttg atggaacctg
     8101 ttcttcttct tttattccat ttgatagtat ctcatcttca attgctcgtg ctatctgaat
     8161 aaatataggt tcttcattat ttaatactaa tttcataggt gcctcctttc tccattagtt
     8221 agttactcaa ctaattaact atataactta taatatcttg aaaaatattt tctgtcaata
     8281 ctaaataaaa aacagaggga aaattccctc tgtttgttgt tctttatatt tataattaaa
     8341 ttttaatttt tgtaatccta tccataagtt aaaaacttta ataatctgtt gtatcttcat
     8401 tttttaatat tttacatatt gagctagttc ctaacctttc tgctcctgcc tctataaatt
     8461 tttctgcatc agaaattgat ttaactcccc ctgcagcttt tatttttaca ttttttccaa
     8521 catgctcttt cataagcttt atatcttcta aagtagctcc acctgttccc attccagtag
     8581 atgtttttat aaaatctgaa cctgacattg ttactatttc acacattttt attttttcat
     8641 cttcatctaa ataacatgtc tctataatga cttttaatac tttatctcct atagcttttt
     8701 ttatttcttt tatttctttt cctatattat cataatcttt attttttata tctgaaatgt
     8761 ttacaaccat atcaacttca tctgctccat tttcaatagc atcttttgct tcaaatactt
     8821 ttgtagcagt agtttgatat cccaaaggga accctatcac tgtacatatc tttattttac
     8881 cttttagata ttctgatgct cttttcacat atgatggtgg tatgcaaact gatgctacac
     8941 tcatatctac agcttcatca cataaaattt taatatcttc ccatgtagtt gtagctttta
     9001 aaatagtatg gtctactgtt tttaatatat gtttcataaa atcctcctaa atttataatt
     9061 aatataatta gattactttt ttaaatgcaa ttttctcttt gcaatattca gtatcatatc
     9121 aacctcttca actttaaaaa tagtcattag tagtgtataa attccacctc caactataac
     9181 tgaaatagct agtgatacaa actcattaaa tgttcctact cccaaaattc caaatataaa
     9241 tttatacgta aagtatgata aaacacccat aattaaagat gcaactaccg atttcaaagt
     9301 tgcttttatt attttatctt gtccaaaata tccaactttt ctttttaggt tcaaaaagag
     9361 taacaatata catgcaattg atgatgaact agttgcaaga gcaagaccac catgagccat
     9421 tggttttatt aaaactaagt ccaatactat atttacccct acagatataa tcccatttac
     9481 cataggtgtt tttgtgtctt gtaatgaata aaaaaccttt cccaaaatat ctcttaatcc
     9541 aaatgcagtc ataccaactg cataaaaaat taaggcagtt gctgtcattt gggttgccct
     9601 cgcatcaaaa gcacctctct caaaaatgat tctaacaaca ggagttgcaa aaaaaataga
     9661 agcaactgaa ataggaatca tagaaattat tatcatattt atactagatt taactgaact
     9721 agtaaacttc ttttgattat tttctgcact taacttagat aacataggat acataacaga
     9781 tactattgaa gctgtaaatg tccctgtaac aaaaccattt aatctatctg cataagtaag
     9841 tgctggaatg cttcctttaa ctaatgttga ggctaaagtt gtatctacca atgaattcac
     9901 ttgattaaca gcaacaccta taaatactgg tgaaagtaat gctagtaatt taataaggct
     9961 atcatcttta aagtttaagt agtataagta tttgtagttc gtttttttaa caaaaaacat
    10021 gtaaaagagc agttgaacta ccatagcaac cacagcacca attggaagta tgtatggtcc
    10081 aaatacggta cttaacatta ttgaaataat tattactata ttatagggta tacttccaaa
    10141 tccaactaca ataaagttct ctttaatttg taaaaatgct gacataacac ttgtaatacc
    10201 tatgaatatt attcctgttc tgaacctccc atgactaaag tcacagggtt cctgcttcat
    10261 agaattttgc atactaaaat atgtcttact aattctccac aggctatccc cgtagttcct
    10321 acggttctta cataagttat ttattttgaa tcgttattaa tcttaatcct tcttttaata
    10381 tattt
//
LOCUS       DAEMHZ010000140        10365 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000140 DAEMHZ010000000
VERSION     DAEMHZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.141"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1896)
                     /locus_tag="KPK72_003179"
     CDS             complement(<1..1896)
                     /locus_tag="KPK72_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2490372.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFD"
     gene            2130..2582
                     /locus_tag="KPK72_003180"
     CDS             2130..2582
                     /locus_tag="KPK72_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2490373.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2585..2893
                     /locus_tag="KPK72_003181"
     CDS             2585..2893
                     /locus_tag="KPK72_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2490374.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3007..4113
                     /locus_tag="KPK72_003182"
     CDS             3007..4113
                     /locus_tag="KPK72_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2490375.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLVRPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4188..6866
                     /gene="mngB"
                     /locus_tag="KPK72_003183"
     CDS             4188..6866
                     /gene="mngB"
                     /locus_tag="KPK72_003183"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2490376.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MIVKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7183..9516)
                     /locus_tag="KPK72_003184"
     CDS             complement(7183..9516)
                     /locus_tag="KPK72_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2490377.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAEMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9491..10207)
                     /locus_tag="KPK72_003185"
     CDS             complement(9491..10207)
                     /locus_tag="KPK72_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490378.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KHIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3859 a         1458 c         1350 g         3698 t
ORIGIN      
        1 atcaaaatca ttacaattag atatactatc tctaatatca ttgtcatgaa ttatctttaa
       61 aaggatatca taaatggagt ttatttttat ataatcatct ttatttatag ccaacatcat
      121 gattaattta acagtagaat cttcactcca atatattggt tcattcaata tggaaataca
      181 aatcttagtt cttatagagc ttaattccat aggatgaggt atagctaaaa aatcatctat
      241 attggtagaa gataaacttt ctctgtaaaa tacagattca gcaaatgatg gaaatacaat
      301 ttcactttga gttaatttgt tgcacatcaa ctttataagc tcatctttat ctttgatttt
      361 aggattaaca ataaataaat ttttatcaaa gaaattgtcc aaaagattac tataaatatg
      421 ggaattatta gtgattgact tagatatatt ctctatatct ttttttaata gttttaaatc
      481 cactaataca ataggaatac tatcatgatt taaaggaatt gtcgatatta ttatatctac
      541 atctgacaat tcactagcta aaaattgatt aaatgataga cattgaagta tatttatttt
      601 atcatgaaaa actttgttta attgaacctc taataaccta cttgaaccat accctgaacc
      661 acatactaat actacgtttt tacacttaat attactttga aaaaatctct ctattccagc
      721 acctatatgc agggatacat atccaatttc atcttctgtt aaagagtaaa tactattttt
      781 aaaaactttt tctatagcat ttaaagttat ttcaaatgct aaaggataat tactctttat
      841 tgtatttata aggggattca ccttatttaa attataagat ttagaattga gaattgattt
      901 aaagtgcagt actaaatcat caaataatac actatcattt ctaaggtcaa aagtatattg
      961 attgttgatt acttctaaaa tttcttcaac atactctata attttagtat ctaccctatt
     1021 ggaaacgtta tccaaaagag aacttttagt tataaaatga ttatatagat aaactctgtc
     1081 tgcctttgaa ataattatag tatatttact ctcaatataa ttaaatatat tttcaacagt
     1141 tgtattcgat tgaaaatctg ttaaaacaac atctaaaaca ttatctaagc agtaaccatc
     1201 aagtattcta cttacagtaa tagctaaatg gataataaag ttttttagat taaaatctag
     1261 gaaattgata ttaaatttat taatttcact taacactacc ctttgaagct ctattaaatc
     1321 tacattatta aataattcta tttctctatc tgtaattcca attatatagt tttcaaaatt
     1381 cctatttatc atattattag aaatacatct tcgaatatct atttccttac cttcaacttt
     1441 aactccataa tatggcttag aaactaatat taaattatac tttgacaaaa taggctttat
     1501 tctttttatg tcatttagta ttgtaaattt actcacatac aagctattag ctaaatcttc
     1561 tagttttata tatgtattgt tatagagaat atattttaaa atatattgat ttctttctat
     1621 aggagaatct ggtatttcac ttgaatccat aatatcatca gatattaatg ctagatactg
     1681 ttggtataat gctaaattaa gaatatctat ataatatcct tctccttttt ttattttaat
     1741 aatagcacca tttttctcta aagttccatt tatagaagta atatcatttc ttatagttct
     1801 ctcactcact tttaaatgat tacttaaagt tttacaagta attttatcat cactttcatt
     1861 taataattta aacaacgtga gcaatctctt gtgcataaat atcccccctt atagaatttc
     1921 ttctaaatta tactatttac actcatataa tacaattact ctaataaaat tgtattataa
     1981 taatttattt ccacttctat atggaaatta gttattatca taatataaat ttccatgtcg
     2041 aagtggaaat tagttatatt taatttaatt aggataatga ttacaatata aatataagtt
     2101 aagacattat gaatggagtg gaaaaatata tggtattaca agaaaaatat atatttatag
     2161 ataaagactt aaaaagcaaa gaagaagtat taaattttat tgcagatagt gcttatatac
     2221 taggtattac aactaataaa gaggctcttt taaaagacct tctaaaaaga gaaaatgaat
     2281 ttccaacagg aataaaagat ggatttgcga tacctcatac aaaaagtgta aatgtaaagg
     2341 aagcttccat actctatatt aaaacaacta acgagttaga gtggaaaact ttagatgata
     2401 gtaaggttag atatgtattt agtttgttag ccccacataa aaatgaagga aatatacatt
     2461 tgaaaactct atctaaatta gccacatgtt taatggagga tgattttaca agtgaagtta
     2521 aaaatgctag tgatgcaagt catttagtaa aatatataac taataaaatg aaggaggaat
     2581 aattatgaaa atagtagcaa taacatcatg tatagcagga ttagcacaca cacctatggc
     2641 agcaaaagct ttagaacaag ctgcatcaaa aatgggtcat gaaatcaaag tagaacaaca
     2701 aggtgctatg ggacgagtaa atactataac aaaagaagaa gcacaagatg cggactttat
     2761 acttatagca tctgaccaaa ctatatctgg aatggaaaga tttgatggta aaaaagtgat
     2821 aaaagtcaaa ataggtatag cgttaaaaaa accagaagct gtcttaacaa agtgtataga
     2881 agctatttct taataaaaaa atctcactct aatatagaat gagaaaaatc aaaaattaaa
     2941 aaattgtcta atataattat aacatattat caaaaaatat aacaaactta tataggagga
     3001 ttaaaaatga gtaaaaaaaa taaatctctt ttaagtgaac ttaagggaca cttaatgaca
     3061 ggtatctcat atatgttgcc acttgtaata ggctcatctt tagttgtagc tatcccaaag
     3121 cttacagctt tagcaatggg tataacaagt ttagatgctt ataaaaatgt atctggtttt
     3181 tatcatattc tttacttaat ggaacaagtt ggttggactg gcataggtct attaaatacg
     3241 gtattagcag gatttatagc attttcaata ggtgagaaac cagctatggg tgcaggtttt
     3301 attggtgggc tcatagcaag taatactaat gcaggattct taggagcagt aatagcaggt
     3361 ttctttgctg ggtatgtttg taagttttta aagaaaaaaa ttaaaatatc tgggtcagca
     3421 tcaggaatga tgccacttat aataatgcca cttataacag ttggattaac tggtttttta
     3481 atgagtgttg tattagtaag acctttagga aacataaata cttcattaaa tgcttgggta
     3541 gctgaaatgt gtcaaaatgg aacaaattcg gtggtattgg ctttaatttt aggcgcaatg
     3601 ataggattcg atttaggtgg accagtaaat aaagcagctt ggatggcagg aaatgcatta
     3661 ttgttagagg gaatctatct accagcgata ttggtaaact gcgcaatagt tatacctcca
     3721 ctaggatatg gtttagctac attcataaga aaatcaaaat tttccaatgc attagctgaa
     3781 acaggaaaag gcaatatagt tatgggtata attggaataa cagaaggtgc aattcctttc
     3841 actttaacaa atccattaaa attagttcct gttaatatga taggatgcgc tttgggtgta
     3901 ggattatcag ctttgttagg tgtacatgct ataatgcctc cagttggtgg attatatgga
     3961 tttatatcaa taggttctgg atgggcttac ttagtaggtg caatatttgg agctttagta
     4021 atagcaatat tatcaacaac tttagtagac ttcaatgaag atgaaactac ggaaaataca
     4081 gaatttgatg aaatagagtt agatataatg taaattaaaa acttaatttt agataattga
     4141 gaattgagtg tactctcaat tctcaattaa attttgggag gatttttatg aaaaagacaa
     4201 aagtacatgt agttccacac agtcattggg atagagaatg gtattttaca acatcaaggt
     4261 caaaaatata tttaatgaaa gattttcaag atatattaga catattggaa gaaaaagaag
     4321 actttaaata ctttactatg gatgcacaag cttctctctt agatgattat ttaaaatgga
     4381 gacctcagga tgaatataga ataagagatt tagttcaaag aagaagatta gttattggac
     4441 cttggtatac tcaaacagac caattggtta tatcaggaga atctataatc agaaaccttt
     4501 actatggaat agatagatgc aatgaatttg gtgaacctat gagggttgga tatgtaccag
     4561 attcttttgg gcaatcagct caaatgccac aaatatacag aggttttgga atagatagtt
     4621 cattattttg gcgtggagtt tctgatgata tggtagatac aacagagttt atatggaaag
     4681 gttctgatgg ttcaaaagta ttagcagttc aaataccatt tggttattac tatggaggta
     4741 atataccaga agatgattca gatttaaaag aatatttaaa agatataatc ggaaaactta
     4801 aggttaaagc ttctactaat aacgtatatt ttccaaatgg atttgaccaa gcaccaataa
     4861 gaaaaaacct tcctgatata ttaaaaaaag ctaatgaaat agataatgaa aatgaatacg
     4921 aaataagtga tatagaaaac tatatagatt cagtaagaaa tgaaagaaat gaatttacag
     4981 agcttaaagg tgaattttta aatgctaaac atatgagaat acacaaatcc atattctcct
     5041 caaggtcaga ccttaaaata atgaataaca aattagaaaa ttatattaca aatgtacttg
     5101 aaccattatt aacattaagt tattcacttg gaaatgaata ccctcaccta gttataaaag
     5161 atatatggaa acttatgttt gaaaatgcag ctcatgacag cataggttct tgtaatagtg
     5221 acacaacaaa tgaggatgta tacacaagat acaaacaagc cagagatttg agtataaatt
     5281 tattagattt gcatatgaga cttataagta ctcaaatcaa aaataataat gaagaaataa
     5341 cattaacagt attcaatgga ttcccagaag tcagaaatga agttgtagaa tttgatactt
     5401 atattccagg tcaagatttt gtgataaaga ataaaaatgg agaaattctt aattatataa
     5461 taaaagaaaa agaagatatt acaaattata tattaactca aacaataaga ttaaacccaa
     5521 gtaaaaaaat atatataccc aaaactgtat atagagcaaa aattgcactt tcagtaaaag
     5581 atataccacc aatgggatat actcaactag tttttgaatt ggatactaaa aataaatgtg
     5641 aaatagaaac aagtaattgc aaagaaatag aaaacaaata ctacaaaata acagccaata
     5701 aaaatggttc attagaaata ttagataaag aatctaataa gacttataaa aaccaagctg
     5761 taattgaaga caatggagat gatggagatt catacaacta ttctcctcca aggaaagata
     5821 tttatgtttc atctttagat tctgtatctt cagtagaagt gctaaaatca aacattcaag
     5881 aagaaatgat agttaaatat tcattgacca taccaacttc actggaagaa agggctatag
     5941 gtaaagtttc tgtgaaaatg ccagtaacaa tgaaaatcac cttagaacct aaagaacaaa
     6001 taattaagtt taatgttaat ataaaaaata accaagcact atctcataga gtaagagttt
     6061 tatttaatac agaaatagca tctaagtttt ctatagcaga tcaacaattt ggaataatac
     6121 aaagaccaac agtattagaa aaagaccttg cattgtggaa aagagacaat tactcatggc
     6181 aagaaaaacc tataactatc gaaccaatgc aaagttttgt tactttagag gatggacaaa
     6241 gaggaatagc attaataact ggttgtgtaa gagaatatca aatagttggt gataatttag
     6301 atacaatagc attaacacta ttcaggtcat ttggatatat gggaagagaa aatttacttt
     6361 atagaccagg aagagcttct ggagaaaaaa taatagcaac accagatgca caattactta
     6421 aagagttaaa ctttgacttt ggtctttaca tatataacaa taaatttgat gaagcaaatg
     6481 tagctaatac agctaagaga ttcttaacac ctatacaaat atatgaatat gctgattttt
     6541 taaatggtag acttatattt gcattcaatg acgaaaaaca aatatatgaa aatgaattta
     6601 gtttgatgaa tgtaaataat tcaaatttca cagtaagtgc aataaagaaa gaagaaaaag
     6661 gagatggaat tgtagttaga atcttcaatg gtatgaaaaa tgaggatgct aagggaagtc
     6721 ttaacatcaa taaaaatgtc aatgatgctt atagtgcaat gctcgacgaa atctatgata
     6781 atactagcaa gcttaaagta aatactaaaa ctgtagaggt aaattcttta tcacattgta
     6841 aagtacaaac aatagtaatt aaataatata aaatagctta tacaagattt caaaataaat
     6901 tgctagtact atgttaatag taaagccaga tattatgcta tttataagct ctgtaacact
     6961 aaataacatt ttgtaaaata aaaaggtgaa ccttgtatat taggattcac cttttttaaa
     7021 taaatcaaca ttctgtttta aaataacatt tataagctgt tttgaaataa tgcttacaaa
     7081 ttattttaaa acaataccta taaattttta tttaaagaat acacataaat atttccagat
     7141 tcttttattc tatagacttt ataattatta aagaagtcta atctaatttt tgttaaaagt
     7201 aattgtaact ttgaataaat caccatccac acttatattc atcttaccac cctgtaattc
     7261 tataaaactt tttgctatcg ctaaaccaag accagaacct tctgtgttac gtgatttatc
     7321 tcctcttaca aatctctcca ttatatcatc tacactaaaa tcaatctcag atgcagacat
     7381 atttttaagc ataatattta ctttattatc tgtttctaaa atatcaatat acactcttga
     7441 atttggcatt gcatatttgc ttatatttat tataagattt tcaaatactc taaatgtacg
     7501 ctgactatct aatggtaata ttattttttc attagaaaag ttattcttta ctattagaga
     7561 tgaatctgca agtttgtcat ctaattctaa aagtgtctgc ttcattagag atacaatatc
     7621 cacatttact atattcaaat aaacatctcc actatttgcc ttgcttactt caaacaaatc
     7681 ttctattaga tgttgcaatc tttgtgactt tctgtctaat gtatcaatat acaacttacg
     7741 attttcatct gttatacttt catcttttaa taaatctata taagtgatta tagatgttag
     7801 aggtgttttt aagtcatgtg atacatttga tataagctct gttttcatct tttgactctt
     7861 aacctcttcg tcaacagctt ttttaaatcc agtttgaata tttcctaatt cgtcttttat
     7921 aggattaaaa aatcctaaat ctttttccat atttacatct aagtttccat ctgaaatttt
     7981 ctttgtaaca tctaaaagtt gattatattc atatattgtc tttttagagt aatctgacag
     8041 tattttaaat aatactactg aatatattaa tgcgagtatt attccaaaga accacatact
     8101 acacattata actataatca ataaatttat tcccaaaatt gtaactatag ttttatttgg
     8161 cttttctttt agatttattg ttctaatttg attaataact gtttttcctg ctttttttag
     8221 ccatacaaaa aacattcctg tcacagtatg ttttgtaaaa tactctctta ttccaacatt
     8281 taaaatatgt tttaaaagta ttataaatac aaatattatt gagaatatta caaaccaata
     8341 agttaaattc attaaactta ccaatatact agccatttca gcacttatgt cattgtttat
     8401 aaggtactct acataattcc cactcaaggt attacttatt ataacagtag gatataaatc
     8461 tacaagcccc tgtataatag caagagcaat aaagagtatc tctaacggta ttcttgataa
     8521 tgtatcaaat cctagcaact ctttagattt tctatatggg aatgctaaag ctacaataac
     8581 aactatagct aatataattt ttaaaatcat tccagaaatc ttataataag aattattttc
     8641 tacattgctt atatatgaaa ttataccatc atcaaaataa gtatttccat aaccatactc
     8701 tacactatca cctttactta ttactttagg tacagcatat actaatgtta tattttttat
     8761 tggagataac ttatatgaaa ttgaatttcc tcctaaactt gtatccatta aatcttctct
     8821 tgctttagat aacaacattt gattaataac tttttgattt actccataag aatccacaat
     8881 atttactttt ccatctttat caaaattcat tacaacgtag aatctatagt tttcttttat
     8941 atctgtaatc tgcttttgtc tttcctcatc attcatttct gtattttttg actgattttc
     9001 atcagaattt tcttttgaag catcattctt tgttgtatca agtactccta attctttatt
     9061 atcattaaaa aattcaaaat tgctttcttt atcaactaaa tagtacttta aattctttcc
     9121 tgacgcttta atgaaatgct cccaattttc aaagttttca ttaaaatctg cattaatact
     9181 atcatctgta taataagttt ctgaaccatc ataagtatct ttttctttac ttacattttt
     9241 tatatagttc atagtttctt cattgataaa tagctttgat ggttgtatca tcttaccttc
     9301 ttgttcttcc ttcatattat gatataaaac ataacttcct ttatatatac tatctacaaa
     9361 gctttcctgt tcaaatggat tctctgctcc acctttagca cttgcttgta tagtagaata
     9421 ttgactaatc atacctaatg atgctgataa cataagtata aaaactataa tagtaactat
     9481 atatttatct ttaaattttt tcaattttgt atccaacacc ccacaccacc tttaaatatt
     9541 taggattttt tgggtcaatt tctatctttt cccttatatt cctcacatgt accataactg
     9601 tatctgtatt tacagcattc tcattccaaa ctctttcata tatttcatca gcagaaaaaa
     9661 ctcttcctgc atttctcata agtaaactta gtattttaaa ttctatagga gttgttttta
     9721 tatgctttcc atcaatacta acttcttttg tattttcatt taattctaat ccacctattg
     9781 caaacacact atcgtcactt tctacctttt gctctttatt tccaaccatg tttagatact
     9841 tagtgtacct tctaagttgt gaattaactc tagccaatag ttctaatggc ttaaaaggtt
     9901 ttgttacata atcgtctgct cctatattaa gccccattat tttgtcaact tcttctgatt
     9961 ttgcagatag cattattact ggaaaattat agttttctct aagcttcatt gtaagtgtta
    10021 taccatccat tttaggcatc attatatcaa ctatagctaa atgtatttct tggttttcta
    10081 taaccttaag accttctaat ccattgtatc ccttaaaaac attatatcct tgatttaata
    10141 gatatatttc tatagcatct gctatctcct tttcatcttc taccaccaaa atattatatg
    10201 aattcattct aatctccttt atattaattt ataaattttc tttacatata tgattataca
    10261 atatgataga agtttttcca tttatatata tatttatatc ttttgttctg gttatttgta
    10321 tttaatgtcc atcttagaaa cctccttcta gtttaaattt aatat
//
LOCUS       DAEMHZ010000141        10352 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000141 DAEMHZ010000000
VERSION     DAEMHZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.142"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            303..1139
                     /locus_tag="KPK72_003186"
     CDS             303..1139
                     /locus_tag="KPK72_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG2490379.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            1423..2418
                     /locus_tag="KPK72_003187"
     CDS             1423..2418
                     /locus_tag="KPK72_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2490380.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(2664..3068)
                     /locus_tag="KPK72_003188"
     CDS             complement(2664..3068)
                     /locus_tag="KPK72_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2490381.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            3909..4823
                     /gene="queG"
                     /locus_tag="KPK72_003189"
     CDS             3909..4823
                     /gene="queG"
                     /locus_tag="KPK72_003189"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG2490382.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRNIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            4929..7166
                     /locus_tag="KPK72_003190"
     CDS             4929..7166
                     /locus_tag="KPK72_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2490383.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            7364..8581
                     /gene="nth"
                     /locus_tag="KPK72_003191"
     CDS             7364..8581
                     /gene="nth"
                     /locus_tag="KPK72_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG2490384.1"
                     /translation="MKNVNLKEINEKDVIVEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     KTEKKTKKSSSSKATQKTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            8775..9245
                     /gene="trmL"
                     /locus_tag="KPK72_003192"
     CDS             8775..9245
                     /gene="trmL"
                     /locus_tag="KPK72_003192"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG2490385.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            9281..10129
                     /locus_tag="KPK72_003193"
     CDS             9281..10129
                     /locus_tag="KPK72_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2490386.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         4314 a          923 c         1932 g         3183 t
ORIGIN      
        1 agtgcttaga taatatacaa agtatgtaag attataatat ttgaaattga ttaaaatggg
       61 tataattaaa gtgtaaatat taaaattaaa aattaatata aattaaatca atgctagaaa
      121 tatgttaaaa gaggaatgag attagttggg atagaacatc ccttagagct tatgaatttt
      181 tagtgaaaaa gtactatcaa ataggaatct ccatcttcat atgttggcta aatgtagtta
      241 aggtggagta gttcaaaatt aaaattgagt acaagtagta gtacaaataa aaggagatta
      301 atatgattaa attaggaaca catctttcaa tagcaaaagg atttgctaat gcggctgaga
      361 cagctgtata tattggtgca aatacatttc aattttttag tagaaatcca agaggaggaa
      421 atgctaaaga atttgatgaa aaagatataa ttaaatttca agaaattaga aaagaaaata
      481 attttgcacc actgcttgca catgcaccat atactatgaa tctgggtggt acaaaggatg
      541 atgtatacga atttgcaaca agggtgatag atgaggatat aagaaggatg gactcattag
      601 gaattgaata catgtgtttt catcctggaa gtcatgtagg tggtggtgta gattttggta
      661 tagatagaat agtaaaagga ttgaacaatg ccataaaggg tgatgaaaat ataactatac
      721 ttcttgaaac tatgtcggga aagggaactg aaataggttt taaatttgag caattaaaaa
      781 gtataataga caaaattgag cataaagaaa aaataggtgt gtgtcttgat acatgtcata
      841 ttttttcagc tggttatgat atagtgaatg atttagatgg ggttttggaa gaatttgata
      901 aggttatagg catagaaaga ttgaaaactg tccatctaaa tgatagtatg atgccatttg
      961 gagacaaaaa agacagacat gctcctatag gggaaggaaa gataggatta aaagcattaa
     1021 ttgattttat ggaacatcct agtctaaaac atttaccatt tttcttggaa actccttttg
     1081 atgatgaagg tcacaagaga gagcttaaaa tgataaaaga aatactatct agtaaataaa
     1141 cctttattaa aaatatgtga aatactccgc cctcaacata attagggtgg agatacaatc
     1201 atgaaatgtg ataatttaaa ttaatggata ggaataacaa aaattgtata caaattaact
     1261 aggtataaag tatgattata ttatgaatag actgatataa aatataatta tattatgaat
     1321 agtatgtaaa tatattgcaa agattctcat aatttgttgt ataattaaag atgacatatt
     1381 tatattatat aaaaaattat taaaagttag gtggggaaag atatgagaaa tttaggcaat
     1441 actaacatga aaataaagag agttggcttt gggggcattc caatacaaag gattactcaa
     1501 gatgatacta acttagttat aaatgagtta gaaaaacaag gaataaactt tattgatagt
     1561 gcaagagggt atacaataag tgaggaagca atcggaattg ccattgaagg aaaaagagat
     1621 aaatttttct tagctactaa aagtatgtcc agagattatg attcaatgaa gagagatgtt
     1681 gaaataagtt taaataattt taaaactgat tttatagatt tatatcaatt ccataatgtt
     1741 aaagaagagg agtatgataa tctttttaaa gataaaatgg cttatagtgc ccttttagag
     1801 gcaaaggagc aaggtaagat aaaacatatt ggtataacta gtcacaattt aaatactata
     1861 gaaaaagcta tagaagatgg aaaatttgac actatacaat tcccttacaa tatagttgaa
     1921 ggacaagcag atgaagtgtt taaaaaagct catgaaaaag gaattgggat aatagtaatg
     1981 aaacctttag ctggaggtgc tttagacaat gcaacactgg ctattaaata tatactatct
     2041 aaagattaca tagatgtagt tataccaggt atggagagtg ttgaacaggt tagacaaaat
     2101 gtcgctgtac ttgaaaatct tgtgctagat gaaaaagata ataaggaaat tgaagaaata
     2161 agaaattctt taggtaaaaa attctgtaga agatgcgaat attgtatgcc ttgtgctgta
     2221 ggaataaata taccattgag ttttttatgt gaaggttatt atactagata tggattaaaa
     2281 gagtgggcta aagaaaaata tgaggttatg gatgttaaac ctactgagtg tattgactgt
     2341 gggctttgcg agagtagatg tccatatgaa cttcctataa gagaaatgct taagactgtt
     2401 gttgaaaagc taggatagat tttatttatt gaaatcggtg tattaaattt actccgattt
     2461 ctttttatta gatgtggtct tatatacaaa tattaaattt acatagttat agctattata
     2521 taaatttaat atttgtttaa tatttactta atgcttttta aaaacatttg tttgagtata
     2581 gacataacac aattattatt gttgtattag tatagtttca aatactggag tatcaatata
     2641 atgtcgaata acacataaaa tctttatttt tcaaaaatat tataggatat atcgtattta
     2701 gatactggat aaagttcaat ttcaacccta gggttatctt tatctataaa atcaaataaa
     2761 agtactggct taagttgttt atcatttttt attattccac ttttttcaat accatcacaa
     2821 atactttttg gataattatg taaatccccc attctcttat ttgggaagaa taatttcaag
     2881 actgttatta gattttcttc aacagtttcc ccttgatact gttggtttat aaaacctgca
     2941 atcatgtttt cgtatgcaag atacttagaa tattttccct ttgaaggcat ccatgcttga
     3001 ccatttatgt tatgaagctt aaaattagat ttactaatag gtctacctgg aattgttatt
     3061 ttaatcaaat ttttcaccat ccttatacag tatttattat aatctgtata tgatttaaaa
     3121 gcaaattgtt atttaaagtg tttttacaga gcttgtttgt aaaattttcc actttagatg
     3181 tagtaatata agataagtat tttgcttaca aattttaaaa tttgatatat gatggacaca
     3241 ttcatattag actattataa gaccttcata atattaataa gtatagttta gtaaagagag
     3301 taaaaagaaa tccatataag tcaagtaaag acttaataaa tggcaatgta aatgggatat
     3361 taattataag aaaaaatcaa tctttttgct taactataga aaaaatgtca aaatataact
     3421 aatgatattt gaagttttag agtttatatt aaaaagtaag aaggttaagt gggtaaaaaa
     3481 ggttgaatag attaaaaagg ctaggtatct aaaagagtta agtaggtaaa aaagttaaat
     3541 aagtaaatga aaaagactaa ttatttaaag gagttaagta gataaaaaag ttaaataagt
     3601 aaatgaaaaa gacgaagtat ctaaaagagt tagtaggtaa aaaactaaat aggtaaaaag
     3661 atgaagtatc taaaagagtt agtaggtaaa aaattaaata ggtaaaaaga cgaagtacct
     3721 aaaagagtta gtaggtaaaa aaattaaata gataaaaaga cgaagtatct aaaagagtta
     3781 agcaggtaag aaaattgaat agctaaaaag attaagtatt taaaagagtt agtagctaga
     3841 aaaggttaaa aagactaaaa aaattaaata aatttaaaaa gtcaattaga ttaaaaagga
     3901 ataattttat ggataataag gaattaaaag cattttgtaa tttaatagga ttaaactgtg
     3961 taggagtagc aggggtagat aagtatgata atttagaaaa gatacttaag gacagacaac
     4021 aaaaaggtta tttaactggt atggaagaac caataattga gaacagaatt aacccaagaa
     4081 atataatgaa ggatgctaac tctataatag tctgtgcatt tccatatcat ataaatagac
     4141 aagaaaagaa taaaaattca aatttatcaa aatattgtca tggaaaggat tatcacattg
     4201 tagtaaagga ctttttacaa caaatttgtg attatatttc taaaacagta acagatttta
     4261 aatacaagtt atttgctgat aatggaccat tagttgacag gtatttagct tatttatcag
     4321 gaataggata ttttggaata aataataata tcataactga tgaatatggt tcttacgtat
     4381 ttatagggta tatagtaaat aattataaat ttgaagaaga taaaccacta gaaaaaagtt
     4441 gttttaaatg tggtaagtgt gtaaaatatt gtccgggaaa tgctatattg ggaaattatg
     4501 atatgaaccc caaaaaatgt ttatcatata taactcaaaa aaaaggagat ttgtcagaga
     4561 aagaaaaagt tgttttaaag aatggcaaaa aggtatttgg atgcgatatt tgtcaagaag
     4621 tatgtccaca taatgttgaa ataccaacaa ctcatatttt ggaatttaaa gaaaatataa
     4681 tagattattt ggattatgat gagatacaaa atatttcaaa taaagaattt aaaaggagat
     4741 atggagatag agcttttagc tggagaggga gaaatataat aaagagaaat atggaaataa
     4801 ttttagataa gtatgatgac taataataag agtctgtttc atatacttta atgtgtattt
     4861 actttaacac ttgttgtgta aaagtcatat tatatcatta aagttaaata tggggaggtg
     4921 ggggaatatt gaaatataat aatacatctg aaataaatcc actagattat atattgggtc
     4981 aagaaagagc tgtagaagct atggaattag ggttgaaaat aaataatcca gcgtacaaca
     5041 tatatatagc tggtgaacct ggaactggta aaagtactta tgccttaaag gttttaaatg
     5101 agtatgcatc caaaaaaaat acgcataaag attggtgtta tatatataat tttgaaaacc
     5161 ctagagagcc tattatagtt gaattagaaa aggggtttgg aagagaactt aagaaagata
     5221 tggagaaatt aattgaaagt cttttagaag attttaaaaa tgcgtttgaa agtgaaaatt
     5281 ttgaaataga aaaaaataaa cttctggatg aatatgaaat agaaaaagat atgcttttga
     5341 agcaaattaa aaaatatgga gaagaaaaag gatttaaatt aaaacagagt aaaatgggaa
     5401 tagtttttat ccctttgaaa gaagaggaag atgaaagtga tgagtctgat gaagagtttt
     5461 acaaagctaa aagagaacta gaaaatatgg cgatacaggt cgtatataaa attagaaact
     5521 tagaagaaat agctgaaaaa gcagtgttag agcttgaaga agagatagct aaacttgtag
     5581 tagaacctca tatcaagaga ttgtgtgaaa aatatgaaaa ttatgataag attcaaactt
     5641 atctggaaaa tataaagaaa gatataatag aatatatgta tttattctat ttagatgaag
     5701 aggagttaaa agataagtat gataaagagc attttattaa atataaaatt aacctatttg
     5761 tagacaatgg aagctcaaag aataaagaat cagcacctgt tgtagtagag ataaacccaa
     5821 gtccagcaaa tctatttgga aaggcagagt atgattatgc aaatggaaat ataaagactg
     5881 atttcacaaa actattatca ggagcatttc atagggctaa tggtggttat ttagtacttt
     5941 atgcagacca attactaaaa tatacaatgt cttgggaaat tcttaaaaag actttgcaga
     6001 ctaaaaaagt aatattggaa acacaaacag caattaaacc agaaaatatg ccattggatg
     6061 ttaaattagt actgattgga agccactata tatatgatat tttatataga tatgatgaag
     6121 attttgaaaa atattttaaa gtatttgtag actttgatag tgaaatggat aaaaatgaag
     6181 ataatgaaga aggaatagca agatttatag cataccaatg tgataagaat aatttaaggc
     6241 attttactaa atcagcagtt gaagaggtta ttaaatttag cactagatta tctggtgatt
     6301 tagaaaaact atcaactaag tttaataaaa taatggaagt tgtaatagaa ggaagtgctt
     6361 atgcagagtt tagaaattcg gagtatacag agcagtgtga tgttaaaaaa gcaataagtg
     6421 aaaagagaaa aagaatcaat agagtggaaa cacatatgga tgaatcaatt gaaaatggat
     6481 ttactctaat agaaacagag ggaagaaaag taggagtgat aaatggtttg tcagtcttaa
     6541 gtacaggaga atactctttt ggtagagctt caaggattac agcgaccact agccccggaa
     6601 gtaaaggcat agtaaatata gaaagagaag taaatatgag tggctctata cataacaaag
     6661 gagtacttat attaggtggc tatctttctg agaattttgc acaggattta ctattgtcac
     6721 taaatgcata tgtttgtttt gaacaaaatt atggtggaat agatggagat agtgcatctt
     6781 ctgcagaatt atatgtacta ttatcatctc ttagtggagt atcaattaaa caaaatattg
     6841 ctgttacagg ttctataaat caaaagggtg atatacaagt agttggtggt ataagtgaaa
     6901 agatagaagg attttattct atatgtaaaa ctaaagggtt aaatggtgag caaggtgtca
     6961 taattcctag aaaaaatcaa agaaatctcg ttttatcaga tgaagttaat gatgcagtgc
     7021 gagatggaaa atttaagata tatactgttg aaagagtaga agaggctata gagatattga
     7081 cagatgttaa atttgaagaa ataaagaaat tggtaaaaga aaaattaata tctttcagca
     7141 agatacaaac tgtgtcaaaa gaataagttt attatagaat ttaatgggta tttatgtata
     7201 ataaaaagca agaactttaa taggtttttg ctttttttgt aaatatatat aaattaaatt
     7261 cttattttgt atgttttata tattgatgtt tatttttttg ttgatatgat taaaccattt
     7321 ataaatagcg ttttataaat tggtattttg aagggggata attatgaaaa atgtaaatct
     7381 aaaagagata aatgaaaaag atgtaattgt ggaagaagag ataaataaaa aaggaatgga
     7441 taagaaaaat ataattgaga aggaagagat aaataaaaaa ggaatagata agaaagatat
     7501 aattgagaag aaagagataa ataaaaaagg aatagataag aaagatataa ttgagaagaa
     7561 agagataaat aaaaaagaaa tggataagaa agatataatt gagaaggaag agataaataa
     7621 aaaagaaatg gataagaaag atataattga gaaggaagag ataaataaaa aaggaatgaa
     7681 taagaaagat ataattgaga aggaagagat aaatgaaaaa ggaatagata aaatgattgg
     7741 taaaaaagat gagataattg atgagaaaga tatagagaat tttatggata ttgaagaaga
     7801 taaaaaaagt atcaaagtaa gtaaaaaaag tactaaaaaa ggtaaaacgg aaaaaaaaac
     7861 aaagaagagt agttctagta aggctacaca aaaaacaagt aaagaaacta aaagtaagtc
     7921 taaaattaaa aatgagtcag caaatgaaaa agacgtaaat aagattttag acgagttaga
     7981 aaaactgtac ccagatgcta aatgtgagtt aaattatggg acagcctttg aacttttaat
     8041 agcaactatt ttatcagcac aatgtactga tgtaagagtg aataaagtta cttcagagtt
     8101 gtttaaaaag tacaacacag ctagagactt tgctaatttg agcattgaag aaattagtaa
     8161 agagataaag agttgtggtc tttacaaaag caagtctcaa aaaataaagg atacatctga
     8221 acagctatgt gaattatatg atggtgaagt acctgatagc ttagaaaagc ttataaaatt
     8281 acctggtgtt ggaagaaaaa cggcaggggt tgtacttagt aacgctttta atcatccagc
     8341 aatagctgta gatactcatg tatttagagt tagcaataga ataggaatag tggacgagcc
     8401 aaatcctcaa aaaacagaat ttgcacttat ggaagctata ccaaaagaaa gatggtcaca
     8461 ctcacatcat gtattaattt ttcatggaag aagaatgtgt aaagctagaa atccagaatg
     8521 tgcaagttgc ccaataaaag aagattgtaa ttattataaa gaacttaatg aaacgaaata
     8581 acttttagaa agtaagttgt acaagaggga ttttctgata taataatact tgtgaaaatg
     8641 ttagataaaa aactattata tacatgaaat aaaaattatt attaacatta ttttatataa
     8701 tagtataata ttattgttat tctgtttata aaataaaatt tgataaataa aagcgatact
     8761 tagggaggat taaaatgtca ttaaatatag tattagtaga accagaaata cctcaaaata
     8821 caggaaatat aataagaacc tgtgcagcaa caggttcaac tttacattta gtaaaaccac
     8881 tgggtttttc attggatgat aaacacctaa aaagatgtgg gcttgactat tgggatatag
     8941 ctgatataca atattacgat agctttgaag aattacaaga aaagtatcca aatagtaaat
     9001 actttttttc tacaacaaag gctaaacagt cacattcaga tgtgaaatat gaagaaaatt
     9061 gttttatagt atttgggaaa gaaacaaagg gattaccaga acctttgttg aaagcaaata
     9121 tggatacttg tattagagtg cctatgttgg atgttgaaaa agctaggtca ttaaatttat
     9181 caaattcagt tgctattgta gtgtacgagg ctttaagaca aataggatac ccaaatatga
     9241 ggtagtttca acaagcctaa atgagaaagg agcatactta atgaaaaatc taaagataat
     9301 ctgtgacagt ttatcggacg tgccaaaaga tttgttagat aaatatgata tagatatagt
     9361 ttcattaaca gttatactgg aggaaagaga atacaaggat agaattgata tatctggaga
     9421 tgaattttat aaaaggctta gagaagaaaa tgtatatcca aaaacttctc aagctactta
     9481 tgcccaattt aaggaaattt ttgagaaata tacaaatgag gatagagaga tactttatat
     9541 agcagggtct gccacagcta caggtacgta tcaaagtgct gtaatggcaa agaatgatgt
     9601 agatggtaaa atatatacat atgactctaa cttgctttgt atgggaactg gtttacttgt
     9661 aataaaagca ggagaacttg caagtgaagg aaagtctgtt gctgaaatat tgcctgtttt
     9721 ggatgaatta aaagagaagg cttttgttat attttcagta gatacattgg aatatttaca
     9781 aaagggaggt agaatatcat ctactaaggc agcaatagga agtatattaa atataaaacc
     9841 gattcttgaa ataagagatg gattagtttc gcaaatatct caagtaagag gaaagaagaa
     9901 tgtaatttct aagatgatgg aattgataaa atcaaattgt ggtgatgatt tatcagacca
     9961 aattgtgtat ataggatata gtgatgattt taaggaaaag gaacaattat ctaaggttat
    10021 gcaagatgaa ttaagaccta aagaaattag atttttccaa attggtgtgt gtataggtgc
    10081 acatgctggt cctggtgtta ctggtatcat atgcttaaaa aataaataat atatttttgg
    10141 gtgacattat ttattttttt attaaattag gatagaatat taattattga gatgttaaat
    10201 tggatttatt ttggatgaaa ttaagtgttg atatattaga tttagtttgt agggaagtgt
    10261 gtaaatagat aagaatctat tgcacacttt tttatagtaa gatttattaa aaaaatttca
    10321 aaatgtactt gtaaattgta tataaaaaaa tt
//
LOCUS       DAEMHZ010000142        10296 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000142 DAEMHZ010000000
VERSION     DAEMHZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.143"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            198..1079
                     /locus_tag="KPK72_003194"
     CDS             198..1079
                     /locus_tag="KPK72_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2490387.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1270..1437
                     /locus_tag="KPK72_003195"
                     /pseudo
     CDS             1270..1437
                     /locus_tag="KPK72_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1697..4264)
                     /locus_tag="KPK72_003196"
     CDS             complement(1697..4264)
                     /locus_tag="KPK72_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2490388.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQSFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     FLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(4267..4872)
                     /locus_tag="KPK72_003197"
     CDS             complement(4267..4872)
                     /locus_tag="KPK72_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2490389.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5258..6718)
                     /locus_tag="KPK72_003198"
     CDS             complement(5258..6718)
                     /locus_tag="KPK72_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2490390.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHMVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7073..7651
                     /locus_tag="KPK72_003199"
     CDS             7073..7651
                     /locus_tag="KPK72_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2490391.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            7943..9193
                     /locus_tag="KPK72_003200"
     CDS             7943..9193
                     /locus_tag="KPK72_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2490392.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLTSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKVTNLHIPFIICALIFVPMAVSVYPFILNIGFPVPFLIFIVSSMLIFF
                     TTSLTSIYVITITQLKTPNELLGKVMSILFSGTSIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9354..9923
                     /locus_tag="KPK72_003201"
     CDS             9354..9923
                     /locus_tag="KPK72_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2490393.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3444 a         1766 c         1490 g         3596 t
ORIGIN      
        1 taatttttat tatttgtact tatttaaggt tacatctagt tataaattgg acaattttta
       61 tgttaatttt atttattcaa attaaagtaa cttacaaact ttccacaata aaaaaccttt
      121 tttattcgtt aaactgctcc atcattggtt tagcaataaa tatacttttt aggtctttaa
      181 tgtcagtagt gttaaatatg ccagcatatt tatttgatag taattctatt acaaatattt
      241 acagagtatt ttctatattg cttagtttta tagttacagg attatatttc agttataaaa
      301 atcataaaaa taataagatt aactttgagg cagttttaga ggacaaaaaa aacttaattt
      361 tcttattgag ccttatgttt ggaatgtact tgtatctatg tttgagtttg tcaatatact
      421 atactgttgg aaatgtattg attttaaaat tatatggaat caagtctgcc ataatagttt
      481 tggtaggtaa ctatattgca gaaagatata gctttagaat tactcaaata aaaggttatg
      541 atattaaaaa tcaaatagaa tatgtagaaa gtgtaaagac agagtatgaa attaataaat
      601 tgaatgatat tgtatataca gatttactga caggcttcta caatagacct tttgtaaatc
      661 aaaaactatc agagtggaca tcaaatcact acaaatttac cttatgtttt actgatttaa
      721 atagcttaaa acctgtaaat gataaatttg gacaccaatt tggtgataaa tacatatcca
      781 ctactgctca aataataaaa aaacttataa atgtagaaaa ttccttacta tttagatatg
      841 gtggagatga atttttattc cttttacaga atacaagttg tgtagaagca gaaaagataa
      901 tgattaatat aaattatgaa ttatcaaaat taagtaatac tgacgagtat ccttatgctc
      961 tttccataag ctatggatta gttgagtggg atggaatatc cgatattgaa actttaatat
     1021 caaaggcaga ttctttgatg tatgaaaata agcttaaaaa taaatctact ttaaactagc
     1081 tagttcagat attctacaaa atatatgtta taagagagta ctaagaatgt caaaatatat
     1141 agattttgca aatattgaat acagtttact aatgaatgca ctggatgtaa gtgttagtaa
     1201 acacctatta gatgaacact ttactgttgt ttgggctaat gaccgttatt atgaaatgtt
     1261 cggctatacc tagctaaaaa gttaaaaatg accactgtag ccgagggaat agagaccagt
     1321 tttcaaaaag atttcttgca agaaataaac tgtgatatgt tacaaggata cgtgttctct
     1381 agacctttac caataaaaga ctttgaaaaa cttatgtttc aaaactcctc caattaacaa
     1441 tataaaattg cttattataa gtgatattat tgtatcaaca aataaaggaa ttgtctcaaa
     1501 attgattttt tctaatcaaa tttttatttt gagacattct catttttttg cataagaaaa
     1561 tccccaccaa gctacggttt ttcttaccgc tgttcggtgg gtattagtag tattatgaaa
     1621 tctgaaaact ttaagtatta ctacttccgc tagcgcaatc acaaaggtcg aaagcttcaa
     1681 agcgaaaata ggctttttac tcatcaaacg tcaccgtacc tgccaacccg tccaccgtta
     1741 tcatttgtcc gctcttaata gctcgtgtgg caatgtgtgt agccataacg gctgggatgc
     1801 catattcacg tgcgacaatg cttgaatggc taagaggacc accaatgtcc gttacaatcg
     1861 cacttgcaga tgaaaataat ggtgtccatg ctggagttgt ggtaactgca accaaaacac
     1921 ttccaggttg aaagctttca aaatctgctg gactattcag tacacaagct ggagccgtca
     1981 caacaccagt actggtacca atgcccgtca acacaatttt cccatctttc tgcttctgtt
     2041 gtgcttgtga tatagttttt ttgtttttct ctggcaactt ggatggtgac acataaccca
     2101 tgtatttttt aagttcagct tttcgaacag gtattatccc gttcatatca gatagagact
     2161 tgtttttatc caactgctct ataagtacct ccaattccga ttttgtgagc caataaatat
     2221 catctaaatg tgaaatagct cctcctcgta tgaatcgttc tgagatttcc tgaaacatac
     2281 aacgaatcag aggatgtccc attcccatca aataaatagc attttcacgc ataggagagg
     2341 tttcctgtgc ccaatggagc aacttaaaaa atagtttttt acgcaatcca cctatgtgtt
     2401 gtaaaatctc ttcttctgca tgttttctac gcttttcaaa cgtggtctgg cgtaaaaaag
     2461 gactttcccc ttttccttcc acaaatgttt ttacggattc aaaagttggt gtaagtgttt
     2521 cctgcggtgt ggaatgcgca aaatcaaact cataagcggt acgaccaaac tctttaaagt
     2581 acttattgat tcgatttttc cattctatcc atacttcctg tgaaactcca acaggcacaa
     2641 ctgaagacat gaaatcttct gctatcttta ttgcaggact gttttgcaga taaaggttga
     2701 tgctgttatt ttgctttacc cattctgaaa taccccacaa ctccttttcc gattgcagaa
     2761 caatcgtatc aaacccaagc aggaaaacgg aagtatccgt tatgccagca cgtcgaactg
     2821 ctccttgaaa taattttgta aataatgttt cactgataga agcagcaggc aatgtaagct
     2881 gaatccttgt gaaataaata catgcggcat aaaatattgt ctgaataccc tccataattt
     2941 gatgggcgtt catagtatgc aggggctttt cttcccattg tttaatagca tcctcatatc
     3001 cttttcgtgc cgtttcccag cgtgcaacgc tgttcataag tgtcctacgt aaagagcgtg
     3061 gggaaaggga tttgatagca atcaaaaatg gctttgattt agcagaaaga taaacatatc
     3121 cattaataac cgtatatgcc ccgttttttg gtaataattt tttcgcactt ttaccaaaca
     3181 tatctatcca taaaagctct gatgcacggt ttacgatttc aagaccaagc gtggcgaaca
     3241 aaggtgtaac aggatttggc aagtgttctg ccaaacttcc ctttgtgtat acaacatcct
     3301 gttctggcaa tacccattct ggtggcagaa cagtaattgg acgagcctga acaatgtaca
     3361 atttatcttt ttccagtgcc cactctacat ccataggcat ttgataatac ttctcaattt
     3421 tctttccaag ctgtatcagt tgcattacct gattacgagt aagagcatat ttttttctca
     3481 acctttcagg aaccatggtt tcttctgttc cttctgaagt acgaaccgtc ataatctctt
     3541 tatttgccac ttcatacgat ataattcgtt cggagtcctt atccactacg attgtatcgg
     3601 gtgtaactaa gctagaaacc accgcttcac caagccccca tgcagcgtta ataatcattt
     3661 cgctacgtct accattgatt gggtttaacg taaacataat accagatgaa tcagaaaatg
     3721 caagcttttg tactacaaca gcaagtgcga caatctcgtg ctttatatta ttcttcacac
     3781 gataagcaat agcacgagct gtccataagg aagcccagca tctctttaca gcgtcaataa
     3841 ctttatcttc accttgtatg ttaagataag tttcctgctg acctgcaaaa gaagcgtcag
     3901 gtaaatcttc agcagtcgca gacgaacgaa cagccaccga tatgttccct aatccagcat
     3961 aagccatttt aattgcgtcc gacacttcct gtggcatttc tccattatgg aaaagctccc
     4021 ctattttctt agatacatct tcaagttggc tggtattatt ggaatcaata ccatctagca
     4081 gtttattaat tcgagactga atatggttcg tctcaacaaa actctggtat gaagcagtcg
     4141 taacatgaaa accgtctggc acagggatac ctgctgtcaa tagttttgat aaagacatcc
     4201 cttttccacc aacaatttca atggttgctt gtttatgttc cagtggcaag acataataaa
     4261 tcataatcat tcacctccat cattcataat tccgtgcaga aatatattca ttgtttcatc
     4321 caacattttt tccgaactat ccaatggtct tgacgtgtaa ctgattgata acatggagtt
     4381 aattaacaaa cgtgtcacaa gagcggtatc cgttttgcga aagatgcctg tggtcacacc
     4441 attatcaatt accgatttta taatatcttg atatgcataa cgagcatctt taagtctctt
     4501 tcgatactct tcttctaaat aatcagattc tgtatttaac cacatcagta cagttctata
     4561 ctgctctgga aatctaagat gattaagcat gatttttcta aggcactgtt gtggtgaagg
     4621 ttcaccatca ataatcctat gaacttgttg gatagttttt tctatttctt tctcaactgc
     4681 atatacaaca atctcgtctt tcgttttgaa aaaatcgtat agacttgact ttcccatgtt
     4741 ggctaaaact gcaatttctc tcattgatgt tttttggaat ccattttcag ccatgagact
     4801 caaagcaaca gcaactattt tttctcggcg ttctgcttgt tgttcgggtg taagcacaat
     4861 tccttttggc atagggcgat acctcctttt agcgaccaat ggtcgtttct tttttattta
     4921 tcatacagcg accatcggtc acttgtcaag gttaattttt ttcattgtat atgctactcg
     4981 agataaaaat gttcatttta gaaattgctc tatttaagta gaaataggct tcatcattag
     5041 aaaacaagac tataaatttg agatatttat catgttcaaa tttcatcaat atcaaagttt
     5101 aagatattaa taaaaaaaga attactaaga aataacatat aaagattcaa aaaaggagtt
     5161 gaacttaaaa ttcaattaca aaaaatcaaa tttaagacaa ctcctttatt ttttatttct
     5221 attattcttt atctctacta ttctttatct ctatttatta caattctcta acatttacca
     5281 ctactgcctt aggtctagtc atagcctcga ttgaatactt gacaccttga gttcccatac
     5341 cagatgcctt tacacctaaa aatgggaagt ggtctggacc tctttcagtc ttattattta
     5401 tttgaacagt accaacttca agtttatcag ctatataaaa tgccttatca atatcacttg
     5461 taaatactga tgattggagc ccatactctg attgattagc aatttcaatt gcttgattta
     5521 tatctttaac tcttataatt ggaagtactg gaccaaatgg ttcttcccaa gcaattttca
     5581 tatcaacatt tacattatct aacaatgttg gatagaataa attatttttt ctaacattgc
     5641 ctgttattaa ctttgctcct ttatgtaatg catcatcaac caacccttga acaaaatcag
     5701 ttgctttatt atctataaga ggagtgataa ctacttcatc cattggattt ccaattgtca
     5761 acttctctac caatggcttt atttttccga ctaatttatc cgccacactc tcttgtacta
     5821 aaattctctt tactgccgta catctttgtc ctgaatatga atacgctcca gatactatat
     5881 tcttagctgc aaaatctaag tctgcatctt ctaatactat agccgcatct tttccaccaa
     5941 gctccaataa tagtggtacc attccagata ttttagatat atgctgacca acttcagtac
     6001 taccagtaaa gttaataaaa ttaattcctt tatgcgttac aacataatcc cctatctcac
     6061 tacccctacc tgtaatagta ttaagtactc catctggtat cccagctttt ctcataattt
     6121 ctaccatatg aagtgcgcta atagcccctt gtgtagcagg ttttaataca actgcatttc
     6181 caccaataag cgctggagca atctttgaca ttgataaatt tacaggataa ttaaaaggag
     6241 atatagctaa aacagtacct aatggaaccc ttgaaacata agacatctta tttcttgttc
     6301 caccagggaa attatctcca ctaatggctt ctccctccat attttttccc acatcagctg
     6361 tgtatcttaa aaaatctgct gttctctcaa cttctgccct tgcagatttt atgtctttgg
     6421 ctatttcaag taccagtata ttagctatct catctatatt ttctaacaat aaatcagcag
     6481 ccttatgaaa tatattggct ctcttgtata caggcatttc agcccatact tttatactct
     6541 cttttgtgtt ttttataacc tcatctacct catgttttgt catagattgt actttcccaa
     6601 caagagaatt atctattggt gagtaaattt ctattggctt tctcgaatta gattctaccc
     6661 attttccatt tattaaattt ttatatacat tttcttttgt tttaagttca ttaaacatat
     6721 ttttattcct ctctctgata taactttgtg tatttaaatt atagccattt attcgttatg
     6781 taaacatttc tttttttcat aaacgtttac ctataaaaaa aattttaagt taacgttttt
     6841 ctagattctt ctattttaat aacatactcg ctccaaatct atataggtca cactttaata
     6901 tcaatatacc aatataccaa tataccaata taccaatata ccaatatacc aatataaata
     6961 ttataaacat aaatcacttg acaaatttat aagtagacaa tattatatat ctatgactta
     7021 aaatattttt aaagtcatag atagcatata caggtagaaa ggattgataa aaatggctaa
     7081 aagttttaca gaaatagaaa aagacaatat aagaaaaaga ctaatttcag aatgtgaaat
     7141 caactggtct aaatatggat acaaaaaaac taacattgat gaactttgtt caaaatcagg
     7201 aatcgcaaag ggttctttct atacattttt tgattcaaaa gaaaagttat tttttgaagt
     7261 actaaatgga atccaaaaca gacttatatc atcagtagat gaaattttaa gtaacatgcc
     7321 tccaaaagaa ggctttgcaa aatccttaaa aatgttatat cgattatatg ataaaaatcc
     7381 atttttatat tcaccgaata atcctgattt catcagccta ataaataaac tacctcaaga
     7441 tatgttttta caacttgagt ccaataacat acaaagtttt tacgacttaa ttgagagaca
     7501 caatctcaag ttgaagatag aaaaagaaaa agcctttggt gtttgtaatt ctctactatc
     7561 aagtctattg gtaaaagaaa atattgaata taactatatt gaagtatttg attttatggt
     7621 agataatctt gtagagcaca tacttgaata acaatttaaa ataatttttc taaatatttc
     7681 tttatactaa agaggattaa tatgattgaa ttaatgaaaa aaataaacaa tatcatttac
     7741 aaaataaatg ataaactaaa tatacacaca aaacttatag accccagagt taactcattc
     7801 cactctactc taagccatct ataatagttc gtatatttct aataatatta gatattaaag
     7861 taagaaaaaa attccttata ataattgcta ttatatcaat tattataaat attttaataa
     7921 tcttagatag gtaggtatat atatggtaaa atcctcaaac acttttaata aaaacttcaa
     7981 cttgatgata ttaggtcaaa taatctcatt gtttggtgca tcaatactta aatttgcttt
     8041 atctttatac atacttgata taactggtaa agcagaaata tttgctacta tactagcagt
     8101 atcttcaatc ccaattatag tattctcccc tattggtgga gctattgcgg atatattcaa
     8161 cagaagaaac ttaatggtta tttttgattt ttcaagcagt cttacagtac taatcttagc
     8221 agtattctta ttcaacaata atggttcaat attacttgta ggaattatta tgaccatatt
     8281 atcagttata agcacaatgt atcaaccaac tgttcagtca agtacacctc ttttagttga
     8341 taatgagcat ttaatgaatg caaatggtat tgtagctgga gttgcatctc ttacaaatat
     8401 tgcaggacct gtattaggag gtgtcttgta tggtgtgatt ggaattaatg caattatagt
     8461 tataagttgt gtaagtttct ttctatctgc aattatggag atatttatac agattccttt
     8521 tatcaaacaa gagcaaaaca gcaacattat aaaaactata tttactgata taaaggatgg
     8581 tattgttcat atatgtaaag aaaatagatt tatactaaaa atactttttt tagctatagc
     8641 aataaactta tttttaactt ctatgttaat tgtaggtatc ccctatatag taaaaataac
     8701 tatgggagct agtaacttct tatatggtct agcagaagga gcaatttctt ttagttcact
     8761 agtagctgct gtgagtattg gtattttctc aaaatatcta aaagtcacta atttgcatat
     8821 tccctttatc atatgtgcac ttatattcgt accaatggca gtttcagtat atccatttat
     8881 attaaatata ggatttccag ttccattttt aatctttata gtatcatcta tgctaatatt
     8941 ttttacaacg agccttacct caatatatgt tataacaatc acacaattaa aaactccaaa
     9001 tgaactttta ggaaaagtaa tgtcaattct tttttcagga acatcaattg caatgcccct
     9061 tggtcaggtt atgtatggta aactttttga tttattatca gatagagtgt acatcattat
     9121 actaggagtt ggatttatta cactacttat agcatttgta tcaaagataa cattgaggaa
     9181 cgaaaatctt tagatacaaa gaatttagat ataaagattt aattttcact aatttctgaa
     9241 tttaaatgca aaattgcgta tcagtatgtt gacgttttag aaattgattg atataatcaa
     9301 tggaaaataa actttataaa aattcaaaat attcagctag aggagtgata aaaatgagtt
     9361 acatgccaac tttagaacaa gcatgggaaa tattaagaaa atacaataag gaagaatttc
     9421 atataagaca tgctcaaatt gtatctggag ttatgagata ctatgcaaag gagtacgacc
     9481 ctgagagagt agacttttgg gaaatagttg gacttttaca tgatttagat tttgaaatgt
     9541 atcctgatga acattgtgtt aaacaaagag aattaatgac agagcttgat ttagataaat
     9601 caataatcga ttctacaata agtcatggat atggattaac tggttcagat gttaaaccag
     9661 agcaatttat ggagaaagta ttatttgctg tggatgaact tactggttta attggtgctg
     9721 ctgcaataat gcgtccttca aagagcgttt ctgatttaga attaaaatct gttaagaaga
     9781 aatttaaaga taaatcattt gctgctggct gttctcgaga cgttataaga agtggtgcag
     9841 aaatgcttgg atgggaactt gataagctta tccaaacaac tatagaagct atgagaagct
     9901 tgattccaga gatggcaata tagctcctcc atacatatac catagaagtt acatctttaa
     9961 agagcatttc tctttttgat gcaacttctt tttatatttt gtagaataaa aagtttaata
    10021 ttttaaactc actatataat ctataataaa tactataaat aatataatgt ataatagtca
    10081 taaactttct aaccttaaaa gtttctctaa tactcaataa acaattctaa tatacaaatg
    10141 gcattatatg atttaattat atatcttcaa tttatatatt aaaataaata tatatatatt
    10201 aaattcttat acaccaaatt tttacattaa acttctatgc accacattta tacatattaa
    10261 attcttatat attaaatttc tatgcatcac ttttat
//
LOCUS       DAEMHZ010000143        10086 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000143 DAEMHZ010000000
VERSION     DAEMHZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.144"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1138
                     /locus_tag="KPK72_003202"
     CDS             98..1138
                     /locus_tag="KPK72_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2490394.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1277..1729
                     /gene="tsaE"
                     /locus_tag="KPK72_003203"
     CDS             1277..1729
                     /gene="tsaE"
                     /locus_tag="KPK72_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG2490395.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1729..2445
                     /gene="tsaB"
                     /locus_tag="KPK72_003204"
     CDS             1729..2445
                     /gene="tsaB"
                     /locus_tag="KPK72_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG2490396.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2435..2911
                     /gene="rimI"
                     /locus_tag="KPK72_003205"
     CDS             2435..2911
                     /gene="rimI"
                     /locus_tag="KPK72_003205"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG2490397.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2917..3933
                     /gene="tsaD"
                     /locus_tag="KPK72_003206"
     CDS             2917..3933
                     /gene="tsaD"
                     /locus_tag="KPK72_003206"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG2490398.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4230..6938
                     /gene="hpdB"
                     /locus_tag="KPK72_003207"
     CDS             4230..6938
                     /gene="hpdB"
                     /locus_tag="KPK72_003207"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG2490399.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6942..7199
                     /gene="hpdC"
                     /locus_tag="KPK72_003208"
     CDS             6942..7199
                     /gene="hpdC"
                     /locus_tag="KPK72_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG2490400.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7192..8142
                     /gene="hpdA"
                     /locus_tag="KPK72_003209"
     CDS             7192..8142
                     /gene="hpdA"
                     /locus_tag="KPK72_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG2490401.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8457..9419
                     /locus_tag="KPK72_003210"
     CDS             8457..9419
                     /locus_tag="KPK72_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2490402.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9518..10086)
                     /locus_tag="KPK72_003211"
                     /pseudo
     CDS             complement(9518..10086)
                     /locus_tag="KPK72_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
BASE COUNT         3959 a         1048 c         1865 g         3214 t
ORIGIN      
        1 taataaacaa aaaataaagt tattattaaa aaactataaa gattagtaat caaattttta
       61 ataataacta atttaataat gatgggagtg tatattcatg aacttatcta aaaagataaa
      121 aaaattattt ttagatgata aagggtatta ttttaatagt aattggaaag atattcatat
      181 tggtatttat aatacaaaat taggagattc tttaaagtat atcactaatg aatatattga
      241 aaatctggaa agggtaaaat atgactatcg tgaattaaaa aatatagaaa atcaaactga
      301 tggtatgtgt attttagcaa taaggcagaa ttacaaggca ttagagtatg taaaaaatca
      361 aaccgaagaa ttatgtttag aagcaataaa gtataattat aaagcacttg aatatgtaaa
      421 agagcaaaca gaatatatat gtcttgaggc tataaaaaag gattgtaatg cacttaagta
      481 tgtacgaaat aaaacagaag ggcttattat aaaagcaata agtcattctt ccaatataga
      541 tgttgtaagt atactaaagg ctttagagac tcaaacaaga aggatatgtt tagaagcaat
      601 taaaaaagat ggcaggtgtc ttgcatacgt tagagaacag agtgaagaat tatgtataga
      661 ggccattaag caaaattata aagcattaaa atatgtaaaa aatcaaacgg aaaaaatgtg
      721 tatagaatct gtaagacaaa atggtatggc attacaatat gtaaataaac aaactgataa
      781 aatatgtata gaagctgtca aacaagatgg aagaagtcta caatttgtca ataataagac
      841 agaagaaata tgtataaatg ctataagata tttaaataaa aaatataata ttaaagatgt
      901 actaagttac atagataagt atacagaaga tatatgtata gagattgtaa gacaaaatgg
      961 aaaaatgctt atgtatataa aaaatcaaac agaaaaaatg tgtattgaag cagtaaaaga
     1021 gaactataag tctcttaagt atgtaaaaga gcaatctgag agaatatgta aagaagcatt
     1081 gaagcaaaac cataaagcaa aagagtatgt aaaaattgct attgatgatt gtatataagg
     1141 tgtagataca gccatcaata gtgaaagata aaaaaagata aagacaagta ctttataata
     1201 atctaggctt atactataat taattaagca gtgatataaa ttcacatgac gattatatca
     1261 aaatggagat gaattgatgg caaaaatata tttagaaaat gaaaataaaa ctagagaaat
     1321 aggatataaa ttaggtaaac ttttaaaaga aggcagtgtt atctgtttag taggagattt
     1381 aggagctggt aaaactacaa tgacacaaag tttggcagat tcattaggga tagaagatta
     1441 tataacaagt cctactttta ctataataaa tgaatatgaa ggaaaaatac ctttgtatca
     1501 ttttgatgtt tatagaatag gtagtagtga tgaaatgtat gatattggtt atgatgaata
     1561 tgtaaattca aatggaatct gtataataga atgggctaat ctaatagaag atatacttcc
     1621 aaaagaatat ttgaatatag aattgagata taaagatgaa ggaagagaaa tgatattaac
     1681 tccaaaagga gagttttata aagagatagt tgaggagttg attaaataat gaaaatacta
     1741 ggtatggata cgtcttctat ggcagcaagt gtagcagttg ttgaagatga taacttaata
     1801 tgtgagttta cagtaaacaa caaaaaaact cattcacaaa agcttatgcc aatgatagaa
     1861 aatatgttga gtatgagtga tttaagtata aaagatatgg atttattagc tgtatgtata
     1921 ggtccaggtt cgtttacagg acttagaata ggtatggcta cagtaaaagc tatggcccat
     1981 gtaaataata taccaataat agcagtaaat tctttggaat ctctagcaaa taacataaat
     2041 ttctgtgata gaaagatatg ttgtatatta gatgcgcaga aaaatcaagt ttattcagct
     2101 aaatacaagt ttgaaaatgg agagatgata gaattagatg gtgttgatgt tgtagagttt
     2161 gatgctttag tagatgaaat tgtatcaact aatgaagaat ttataatagt tggagaagct
     2221 gtatacaaat ataaagacaa gttagagaat ataaaaaata taaaaatacc ttcaccagct
     2281 aataatgtat ctaacgcagg aagcttatgc tctttagcat taaataaata taataaaaat
     2341 atagatgtac atacttgtta tacgataaat cctatgtata tcagaaaatc tcaagctgaa
     2401 gtacaatatg atgaaaaaat gaagaggcta aataatggaa gataatacaa aactaatagt
     2461 agatgatata aaaatagaag aaatgactac agaagatata gatgaagttt ttgaagtaga
     2521 aaaaaattgt tttgaagatt attggtcaaa agagtctttt agaaaagagt tgtctaatga
     2581 agtagcaagg tatatagtag ctaagttaaa tggaaaaatt gtcggatatg tgggaatttg
     2641 gcttatactt gatgaagggc atataaataa tgtggctgtt catagtgact atagagggaa
     2701 aaaaataggg gacaagttga taaaaggcat agttgattta tgtaaagata ataatattgc
     2761 atctatgaca ttagaagtta gagcatcaaa taaaatagca caaaatttat atagaaaata
     2821 tggatttaaa atgggtggaa taagaaaaga atactacagt gataataaag aagatgcaat
     2881 tataatgtgg aaccaattaa aagaggtgta gagtttatga gtgatataat aacattagct
     2941 atagagagta gttgtgacga aacagctgca tcagttttaa aaaatggtag agaaatactt
     3001 tcaaacataa tctctactca aatcgagaca cataaaaaat ttggtggtgt agttccagaa
     3061 gtggcctcaa gaaaacatgt ggaaaatatt gatatagttg ttcaagaagc tttagacaaa
     3121 gcaaatatag gttttaatga tatagaccat atcgcagtta catatggacc tgggctagta
     3181 ggtgctttat tggttggtct atcttatgct aaagcacttg catatacttt gaatattcca
     3241 cttgtaggag taaatcatat agaagggcat ctaagtgcaa actatataga gcataaggat
     3301 ttaaaacctc cttttataac attaatagta tctggaggtc atactcactt ggttgaagta
     3361 aaagattatg gcaagtatga aattttagga aaaacaagag atgatgcatc tggagaagca
     3421 tttgataaga tttcaagagc tatgaattta ggctatccag gaggtcctat tattgataat
     3481 ctggccaaga atggaaataa acatgctata gaatttccaa gagcctattt agaagaagat
     3541 agctatgatt ttagttttag tggattaaaa tcttctgtat taaattatct aaatggaaaa
     3601 agaatgaaaa atgaagaaat agtagttgaa gatgtagcag catctttcca agaggctgta
     3661 gtagaggttt tatctactaa agcactaaaa gcagtaaaag acaaaggata caatatcata
     3721 actctttctg gtggggttgc ttctaattcg gggcttagag ctaaaataac agagcttgct
     3781 aaagacaatg gtattactgt taagtatcct cctctaattt tatgcacaga taatgcagct
     3841 atgatagggt gtgcaggata ttataatttt ataaatggga aaacgcatga tatgagttta
     3901 aatgcagttc caaatttaaa gataaatcag tagagttaga tgaatttgta aatataaaag
     3961 tattgtttca aaaaagtaag tagaagtgaa attaaatata ataaaaataa atatagtata
     4021 tattaaaact tgaagtaaaa ctatttataa tctttatatt ccaatgaatt gcaacaaatt
     4081 ttaggatata cagattgtct ttggcttaga tttgcttcaa gtttattttt ttctatagaa
     4141 agattatttt aaaaagtggg taatataccc ttttttttca ttcttaaaaa gttttatact
     4201 aattatagaa agattaagga ggggaagaaa tgagtcaaag taaagaagac aaaataagaa
     4261 gtattttgga agcaaaaaat attaaatcaa attttcaaaa taaagaaaat ttaagtgaat
     4321 ttaatgaaaa aaaagcaagt aaaagagcag aagacttatt agatgtatat tataatacat
     4381 tatctactgc tgatatggaa tttccatatt ggtataatag agaatataga aaatcagatg
     4441 gtgatatacc agtagttaga agagctaaag ctcttaaagc tgctttttca catatgacac
     4501 caaatataat acctggtgag aaaatagtaa tgcaaaagac acgccattat agaggttctt
     4561 ttccaatgcc atgggtaagt gaaagtttct ttgttgctca aggagaacaa atgagagaag
     4621 aggcaaagaa attagccagt aacacagctg atgaacttac aaaatttggg tctggtggag
     4681 gaaatgttac tgaaagtttt ggaaatgtcg tttcaatagc tggtaaattc ggaatgagaa
     4741 aagaagaagt acctgtactt gtaaaaatgg caaaggaatg ggttggaaag tctgttgaag
     4801 atttaggttt tcactatgaa aaaatgatgc cagattatga tttaaaagaa aacctaatga
     4861 gtacacttat atgcatgttt gattcagggt atacacttcc tcaaggtaga gaagttataa
     4921 attattttta tcctttaaat tatggattag atggaattat agaaatggca aaagaatgta
     4981 aaaaggcagt tgcaggaaat gcatctggag atgggcttat tggtatggac cgtctatatt
     5041 tttatgaggc tgttattcaa gttattgaag gattacaaac ttggatttta aattatgcaa
     5101 aacatgcaaa atatttagaa agtatagaga cagatttaga agctaaaaaa gaatactcag
     5161 atttagtgga gatattagaa catattgcac ataaacaacc tcgtacattt agggaagctc
     5221 ttcagttgac ttatacaatt catatagcat cagtaaatga ggatgctata tctggtatgt
     5281 ctataggacg ttttggtcaa attttatatc cttggtatga acaagatata gaaaaaggac
     5341 taattacaaa agaagaggtt atcgaattat tagagcttta tagaataaaa atcacttgta
     5401 ttgactgttt tgcttcagcg ggcgtaaatg gaggagtact atcaggcaat acatttaata
     5461 ctttatctat aggtggatta aaagaagatg gttctacagg agctaatgaa ctagaagagt
     5521 tattattaga agcaagtatg agatgtagaa ctcctcaacc tagtttaact atgctatatg
     5581 atgaaaagct tccagaagat ttcttaatga aagctgctga atgtacaaag cttggttctg
     5641 gttatccagc ctgggtaaac aactcaaatg gaacaacatt tatgatgaaa caatttgctg
     5701 atgaaggtat gactgttgaa gaagcaagag catttgcatt aggtggatgt ttggaaacgt
     5761 ctccagggtg ttggaaacag ttaactttaa acggaaaaac gtactctatt gcaggtggag
     5821 caggtcaatc tgctggctct ggagttcact ttatagctaa tccaaaaatt ttagaacttg
     5881 ttcttatgaa tggtaaagac tatcgtatga atatacaagt atttgagcct cataataagc
     5941 cattagatac ttatgaagaa gtcatagaag tatttaaaga ttattataaa caagcaataa
     6001 atgttttaga aagagcaaat aacattgaat tagatatatg gagaaaattt gatacttcta
     6061 taataaattc actactaaag ccagactgtt tagacaaagg tcaacatata ggaaatatgg
     6121 gatatcgtta taatgcaaca ttaaacgtag aaacttgtgg tactgttact atggtcaatt
     6181 catttgcagc tcttaagaaa cttgtatatg atgacaaagc atttacaata gaggaaatga
     6241 aggatgccat attaaataat tttggattta aagatgcatt agaagtagga aattattcta
     6301 tggcagacca agtaaaagta gataagactg gtaaatatga tgctatttat aaggcctgtc
     6361 ttgatgcacc taaatatgga aataatgatt tgtatgcaga taatattctt aaaaactatg
     6421 aggtatggtt atcaaaagta tgtgaagaag cacaatcttt atatgctaag aagatgtacc
     6481 cttgccaaat atctgtttct acacatgggc cacagggagc tgctaccttg gctactccag
     6541 atggtagact aagtggaaca acatattcag atggttcagt ctctgcatat gcaggaacag
     6601 ataaaaatgg agtatatgca ttatttgaat ctgcaactat ttgggaccaa gcagtagttc
     6661 aaaattctca gatgaattta aaacttcatc caactactat aaaaggacaa caaggaacta
     6721 aaaaattatt agatttaaca agaagttatt taagaaaagg tgggttccat attcaatata
     6781 atgttgtaga ttcagaaaca ttaaaagatg cacaaaaaaa tcctgacaat tatcgtcaat
     6841 taatggtacg tgttgctggg tttactcaat attggtgtga attaggtaaa ccaatacaag
     6901 atgaagtaat tgctagaaca gagtatgaag gggtgtaaaa tatgagaaag catagtgatt
     6961 gtatgaattt ttgtgcagta gatgcaacca aaggaatttg tagattatca aaacaaatga
     7021 ttaatttaga tgatgcagca tgtccagaga taaaagtaat gccaaaatgt aaaaattgta
     7081 aaaattttgt tgaagctaat gatgaaggta taggcaaatg tgttggccta gagaaagaag
     7141 actgggtata ttcaacattg aatgcaatta cttgtgaagg acatgtgttt aatgagtagt
     7201 caaaagcaat tagaaggcat gatttttgat gtacaaagct tttcagttca tgatggtcca
     7261 ggttgtagaa caactgtatt tttaaatgga tgtccactga gttgtaaatg gtgtgcaaat
     7321 ccagaaagtt ggactgtaag accacatatg atgtttagtg aattatcttg tcaatatgaa
     7381 aatggatgta ctgtatgtca tggtaagtgt aaaaatggtg cattgagctt taatcttgat
     7441 aataagccag ttatagattg gaacatctgt aaggactgtg aaagttttga atgcgtcaac
     7501 tcatgttatt ataatgcgtt taaattatgt gcaaaacctt acactgtaga tgaacttgtg
     7561 caagtaatta aacgtgattc taacaattgg agaagtaatg gaggagtaac atttagtgga
     7621 ggtgagccac tacttcagca tgaattttta catgaagtat tattaaagtg tcatgaggta
     7681 aatatacaca ctgccattga aacgagtgca tgtgtatcaa atgaagtttt taataaaata
     7741 tttaacgata ttgattttgc atttattgat ataaagcata tggatagaga aaaacacaaa
     7801 gaacaaacag gtgtgtataa tgatttaata ttagaaaata tatcaaacct tgcaaattct
     7861 gattggaatg gaagattagt acttagagta ccagttatct ctgggtttaa tgatagtgat
     7921 gaaaatataa gtgatattat atcttttatg cacaaaaata atttggtaga aattaatcta
     7981 ttaccatttc acagattagg agaatcaaaa tggactcaat taggcaagga atatgagtat
     8041 tctgataagg gtgatgttga tgaaggtcat cttgaagaat tacaagatat atttttggat
     8101 aatggtatag cttgttatgt tggtcatgag acagctttct aaaattaaat acaagtttta
     8161 attaaaaaag gatgttacac taaaagctag agttacatcc ttttttatgt ggtttttatg
     8221 tagaaataat taagtaaagt tttatatgtt ttatatattg tttatattgc tttatgtcca
     8281 taaatattgc tttaaatata ctttatatat tttttatagt ttaaattaca gttagagaat
     8341 atattacgtt tacttcattg aagcagaagt tttgtggtaa aatactacta tgaaaaaatt
     8401 taatagaata tattttagaa acaactaaaa ataatagtta ggaggaagac atctatatga
     8461 acaaatcatg gagcaacttg attattaaga ataaagacag aaaagtacgt tcagggtgga
     8521 ttattatagc tgttatggct accttttaca tacttcagta ttatttaagc atgatacttg
     8581 tggaaataat aagaaaaatc ctggttgcta caggtgacat taatttaaaa acaaactatt
     8641 tttcagaata tgctaattgg ttaaatgatg ttgggttaca gattgtattt caaattctaa
     8701 cagattcact tatgattatt attcctataa ttgtatggag atttattatg aaaaatccta
     8761 ttagtgaaat gggattacat tcttatagaa ttaagaaaaa agaaggatat gtaggaatgt
     8821 tattaggaat atttaattgt acagtaatct ttattataac aataacaatt ggtggtggtc
     8881 atgttacttc atgggtacca aaaatttcac ctttaacagt ttcttggata cttgtcttta
     8941 ttttagttgc ttttgcagaa gaaattttaa atcgtggttt ttttatggca gttttacgaa
     9001 gatgtaaaaa tatttatttt ataatgattg ttccatctgt tatttttggg ttgattcata
     9061 tatggaatcc tgatgttact tttttgtctg taattaatat tataattatt gggattcttt
     9121 tttcatatat gtttataaag tcgtcgaata tatggatgtg tataggttat cattttacat
     9181 ggaatgtatt tcaggggatt atatatggta tgcctgtaag tggacttcag gttccaggga
     9241 ttataactac acaaatcact catggtaatc tattaaatgg tggtatgttt ggtattgaag
     9301 gtggaatttt gactactttt gttaatctac ttagctttat atttgtatgg tattactata
     9361 gagattctaa gtatgatttt gttagtgata aagtaaatgt gaccaatatc aagaattagt
     9421 tgattataat aaattttaat aatatcttct aagcttaaag tattctgtag gattgttgga
     9481 aatttacaaa aatataaaaa taacttggat attctattta ctccaagtta tttttatatt
     9541 cttttgtaaa tagaaatgga acataaaaaa ttaaaacaac catagatact attagtatat
     9601 catggtaagt gatagttgat tctttagaat ttgtgataag aaaagcacta ttatttaaaa
     9661 catgaattat agcacaaaac cagatattct tagtttttat ataaagatat cctaaaaata
     9721 ttgaaattcc aactatatgt attgaacgta atattattcc taatattggc acttcaggtg
     9781 tatataatgt aaatattagt ggtaagtgcc acaagctcca acacatacct aatattataa
     9841 ctcctaactt tttaccaaat ttatcaaaaa aaaatattct gtaaaaaacc tctccaggca
     9901 tattcttcac taaaaaaaat ataaatagta gttaatgatg ttattggagt aaatataaat
     9961 tttatgatac cactataatt aggttgagcc ccttgtagta ttagtataaa tgatgttgga
    10021 acaattacag aaatcataaa gtaaataatt aataaagaac ctagtttaat attttttaca
    10081 ggatat
//
LOCUS       DAEMHZ010000144         9872 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000144 DAEMHZ010000000
VERSION     DAEMHZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.145"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..1570
                     /locus_tag="KPK72_003212"
     CDS             119..1570
                     /locus_tag="KPK72_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG2490403.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(1774..4287)
                     /locus_tag="KPK72_003213"
     CDS             complement(1774..4287)
                     /locus_tag="KPK72_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2490404.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALTKGVNYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(4510..5331)
                     /locus_tag="KPK72_003214"
     CDS             complement(4510..5331)
                     /locus_tag="KPK72_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2490405.1"
                     /translation="MIDINLEKCVGCRMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(5826..6302)
                     /locus_tag="KPK72_003215"
     CDS             complement(5826..6302)
                     /locus_tag="KPK72_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG2490406.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(6337..6915)
                     /locus_tag="KPK72_003216"
     CDS             complement(6337..6915)
                     /locus_tag="KPK72_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2490407.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(7019..9619)
                     /locus_tag="KPK72_003217"
     CDS             complement(7019..9619)
                     /locus_tag="KPK72_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2490408.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTVVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT         3145 a         1743 c         1110 g         3874 t
ORIGIN      
        1 atgctttatt tgtaatataa tctgttaaaa aatcttcttt aatattttta aaggagttta
       61 ttttagctta taaaaaaaat agctacttat atagataaat taaggaggaa catgagtcat
      121 gggaaacgta ttagaaggat taaaaccaga aagtgttttt aaaaactttg agaaaatatc
      181 tcaaattcct agaggttctg gaaatgaaaa aggcataagt gattttttac ttagctttgg
      241 taaaaattta ggtcttgaaa ctattcagga tgaaagctta aacataataa taagaaagcc
      301 tgctacaaaa ggatatgaaa attgtccagg tgtagttctt caaggacata tggatatggt
      361 ttgcgaaaaa gaaaagaatg ttgaacatga ttttcttaaa gaccctataa aattaagaat
      421 tgatggtgac atgatatatg ctactggaac tactctagga gctgacaatg gtatagctgt
      481 tgctatgggt atggctatat tagaagataa tactttagag catcctgcac tagaagtttt
      541 agttactgtt aatgaagaag atggtatgaa tggtgcagat gcactagacc ctagcttaat
      601 taaaggtcaa tatatcctaa atatggactc cgaggaggaa ggatacttat tagttagctg
      661 tgctggtggt aaaacttgtg ttgttagttt acctgttgaa tacaaggaag ttaaaggtga
      721 caaacaagga ttattagttg aggttactgg actattaggt ggacactctg gtatggaaat
      781 tgttttacaa agagcaaatg ctaacaaagc aatagctaga gtattaagtg tattgaatgt
      841 tgattatgaa ttagcttctg ttgatggtgg tactaagcac aatgctatac ctcgtgaagc
      901 taaatgtgta atagctgtta ataaagctga tgttgaatct gctaaaaaac aaataaatga
      961 cattttaact gcatttaaac atgagtttac tacttctgac cctggcatga cttactcagt
     1021 agctgaaact tctgtagaca aagttcttac taaagattgt aaagaaaaag ttgttcaaat
     1081 gtcttgctta actcctcatg gagttcaatc agtaagtctt gatatagaag gattagttga
     1141 aagttctact aacttcgcta taattgaaac taaagaatct actattgaat ttttaacttc
     1201 tgttagaagt tctgttatgt ctattagaga tgaaatcgct gatagaataa gacttttggc
     1261 tcaagcttta ggtgctaatt acgaccttat agctcaatac ccagcttggg aatttaaaaa
     1321 aggctcaaaa ttagaaaaaa tatgcagtga aacttatgag aaattaactg gtaaagttcc
     1381 tactgttatg gctctacatg ctggtcttga gtgtggatta ttacttgata aacttcctca
     1441 tgctgaagct atctctatag gaccagatat gtttgatgtt catactccaa atgaacatgt
     1501 tagtatccct tctgtagcta atgtttggga ttatgttatt gaaatattaa aatctatgaa
     1561 ccaatattaa atttaagttt tgattctaag tagattttaa ttttaagtaa tctatataaa
     1621 attaactctg taaattaata ctacggttag tataatattt actttcaata ttaacttata
     1681 gaaataaagc ctaatgatag ataataacat gtatcattag gctttattgt aatttaaata
     1741 ttattaagct ttattctgtt ttaaaattaa ttcttatact attttaaatc tatttcttcc
     1801 ttgtctctta gaatcataaa gagcattatc tgctatttga tacagttcat catactcttg
     1861 accaggcttt gctataacta cccccatact aatagataaa ctttctccat ttggcaactt
     1921 aatgagatga atatgattgt atacatcatt acatttatta ataagttctt ccttatttat
     1981 tatatcattg atataaacac taaattcatc tccacccaaa cgacctacta tatcattttt
     2041 tgcaaaactt tcatcaagta cctttgcaac atcaataagt actttatctc ctaacaagtg
     2101 accataagta tcattaatcg acttaaaata atcaatatct ataatcaata aagcaccatt
     2161 tttttcagtt gataaattca acaaattttg tgcagttaat tttctaatat atgctgcatt
     2221 ataaatattg gttaaactat ccctttgagc cttatctaac aatctattct tttcctcttt
     2281 ttcagaatca atattacata ttttcccgat tacatacatt ggtatttttc tatcattata
     2341 aattattttt gaagtaactc taatccaatg aaaagtatca tcaagcataa tagaatacat
     2401 ttcagttgtt ccattttctt gtgattttat tacatcaaaa aactttttat tatattcttt
     2461 ccttatttta tctctttcat tttctatatt gccattttca ctttccttat catttttatc
     2521 caaaccataa taagtctcaa tacccttttc tggcatagaa attatcaatt tatcttgctt
     2581 ataatcatac tcaaaaaagt attcacttgt catttcatac aactgagtat gttttttgag
     2641 ttctaaagat atcttctcgt tcatacttgt acgagtatgc aaaccaaatg ccataaatcc
     2701 aataatacaa attaataaac tggctacaat aaaaatagtc tcaattggat tctgttgcat
     2761 aatataaagc aacgagaaat cttgtctata tgtagtattt tggtagacaa ttgattgcat
     2821 tttttctgat gaaatactta taatagcttt attaaatata ttcaataatt cttgtttatc
     2881 ttttttaatt ataccaaaac aaatactctt tgctttatag gtttgaggaa ctagacgtac
     2941 atttttatac ttaggttgat tcatatagta ctgcatagaa taaccttctc cataagtgta
     3001 atctgcatag cctttattaa cagcttctat acactcttct aaattatcat accaaactat
     3061 attctcttca aactggccat aataattaac acctttggtt agagctatac gttttccatt
     3121 tagactattc tcatttaatt tattattaat catcatagta tattgagtag aaagaaaagg
     3181 ccgagacata gctatattat gttcacgaga gtactcataa tcataaatca ttcctgctac
     3241 aatatcaatt tcatgttttt tagccatatc ttccaaatct tcttgagatt ttacaggcac
     3301 aagttcaaat tccaaacctg ttttatcaca tatatgattt aacaaatcaa ttgatatacc
     3361 tcttaattct cctgtatctt tatctgcgtt ttgaaacggt ggtttattta aaagtactcc
     3421 tacttttaat ttttctgtat ctttaatgta attagtttct tcatcagata aaaacatttg
     3481 attattctct ggcgtaaagt atttctcata taaagtagtt gaaaaatatg ggtccttctg
     3541 ttcaatatta ttaattgctg aattaagctt ttgagttaag tctgtactgc cttttggggt
     3601 aataaaataa aatggctttg atgagaattt tgctacagta cgtacacctt ctattgggtt
     3661 catgctaaca ttaagaagta catctgcttt accactcttt agtacttcta attgctcgtc
     3721 atttgtttta caatatacca attcaggtga catcagattc atcttacaat actcatctaa
     3781 ctctttaagc ctagtatcag aattttctat tacagcaata cggatttttt tcacatcttg
     3841 tgaactcatt atgttatcaa tatctgtatt ttcatataat gcttgtagta ctgtatttgc
     3901 tactccataa ttattccctg cataattata tattttttgc attggctcat tatataacat
     3961 ccctcccatt atatcaattt caccattttc taacatcttc attaattttg taatagattc
     4021 atttatctct ccaggaactt gaacaaattc ataattccat ccagtatact gtgcaatttc
     4081 tagtaaatat tcatatgtat acccaaaata gttaccttgc tcatctattt cagttaaacc
     4141 tttttgaata ggataagcaa cacgaatggt acgattatca ttacctatag cgttgatacc
     4201 aaaagaaaat atgcaagaag tgaatataga tgtaattaca ataattaata aaagtaattt
     4261 ttttaaattc actacacaat cctccatata gttaagattt acagtataaa ttactgcata
     4321 aacttgtaga catgaaaaaa tagattagta tataaaagga tatataaatc tacttagaga
     4381 tttaaatatc cttaatatat acaatatatt taaattataa ctcatttata tgtctattac
     4441 aacaaattta ccctgctaaa tacctactct atatcccata attttacaag ttgtagtgaa
     4501 gtttaagtcc tatctcacct caaatttagc agtgtttcta tttacagttc ttttatattt
     4561 cacatctgga taacccaata taaatgaagt gataactgct tcgttctcgc tcattcctaa
     4621 cagttctttt atctttggct ctacatttga tgccataaca aaaaatccat tataacaaat
     4681 tcctaatcct tgagaatagg ccataagttc catattagat gcagccagtc ctccatctac
     4741 ataagcactt ccacctagag acatatctcc tacaactacc ataagtagtg gggcatcata
     4801 aaataaagaa tcatttccgt tctctttata tcttttgtac attttcttaa atgtattttt
     4861 atacatagaa cgtacagggt cattgtcatc agtatctaat gccaaattat aaagaccttg
     4921 tattgccagt tctttcactt cttttagctt ttcttttact aatatgtatc ttataggctg
     4981 tctattacca cctgttggtg tgtatctacc agcttccact atgtttttta ttttttcttc
     5041 ttctacctct atatctttat attgtcttat acttcttcta aatttaataa aattcaataa
     5101 cttttcgggt tctaattcaa aactttcacg attgtattct attacttcat ttttatcaaa
     5161 tccttgcatt tctattgcat ctgttgggca aattgccata cagtgaccac agttaataca
     5221 gagaatattt ttaacctttg ctttatcatc cttaactttt attgcattta caagacagtc
     5281 tgattcacac atcctacagc ctacacattt ttctaaattt atgtctatca ttccaaatta
     5341 ttcctccttt taaaaaattt actttagtat aaaacaaata gttgtctaag tcaattttta
     5401 tttttttaac aatatactaa ccttaactac actttaatgg agaaaaatat acataaattg
     5461 gaacttaaat tagaacttat tcaaagtaaa agataaaaga agtagatgtc taaaagtgaa
     5521 ctttttagtt aattagatat tctttattgt atgaaatcaa gtggatattt atcatttcaa
     5581 caataaaaaa ggcttacctc tattttggat atgtccctgt caagtagaca tactaaaaaa
     5641 aggctatctt tatgcactga gatagcccct tttcccaact actattgttt atcttaatat
     5701 tagtcaataa atttaatttg ctataacctg taagttacat tttaatttga tgtaacttta
     5761 aattacattt ttaagtaata tctttaaatt atattcttaa aatatatcat taaactacat
     5821 tttaattaat ctaagaaact tacattataa tcttttagtt tcccattatc atatacaatc
     5881 tttgcactaa agatatttcc agtcttagcc caatttaaga acagcctgtc accttcccac
     5941 atatttaaat cgaatatttt atcattatca acccaaatta aatttccttc tttacaatca
     6001 tttaaatctt tttcagataa ttcaccttca aactcatctg agaaatacaa aaacatatac
     6061 caatcttctt catctttaaa ttttggaaaa gttatcaacc ctttatatga caatgattta
     6121 agagtaagac cagtctcttc ttttacttct ctaattacac attcttctgg agtctctcct
     6181 tgctcaaact ttcctccaac tcctacatac ttaccttcat gtatatcatc tttcttttta
     6241 tttctgtaca gcatcaaagt cttgtcatct ttttctatat aacaaatagt tgtaagtttc
     6301 atgtatctcg ccttgctttc tcttttatat tctcaactaa ttattttcag cactttcaag
     6361 cacatcactt atattcttca ttgcattaag agaaagctct gcaaattcat caaatggaat
     6421 ccctatatct tctatacttc tcattgcatc tctatttaca tttgaagcaa atctcgtttc
     6481 tttcattctt ttaataacag acttagtctt tacactggta agttttttat ctggataaac
     6541 taaagctata gcagttataa acccagttat agggtctgct gcatatattg ctttttccat
     6601 taggctagtt cttgtaactc ccattccatc atgatgacca agtattgctc tatacatttc
     6661 ttcgtcaccg aagttttctt ttttaagcag ttctactgtt gttgtagcat gtccctcaca
     6721 tatttctcct ggctttcttc caactatatc tgaatccaaa tcatgtaaaa gacctgccac
     6781 tgcccactct tcttcttttt ctggctctag tcttttggct aattctctca taacagcttc
     6841 aacagcataa caatgttttc tcattctatc agtttctaaa tacttaaata aaatttctaa
     6901 agcttcttct ctcattttca ccaatccccc tgttttttca catatatgat atttttatat
     6961 attaatacac ataaattcaa gcttttatat atattcatgt atatttttca tgttatatct
     7021 attctacacc tttatagtag tgtcctgcaa ctgttccaat ttcatctaat ttcttataca
     7081 agtctgtagc cttttttcca agtttataat tattactcaa tacctcaatg tgagcttcaa
     7141 taaccttttt aaccatctca ctatcttcaa ctgtaaaatc tatcctaaat acatctattc
     7201 caatttcctc taactcatat agattgtcca acatacaagt aaccttcgaa ttgtaaattg
     7261 tacttctaca gaatatatct tgtgttatcc tataacttgc atccgtaaag tctcttaaag
     7321 cataactact ttccttacat ttagcaactc tcttgtcctt tttacaatct ctaacaacta
     7381 ctcccatagg acagtattct gtaaccatca taggagcata tccatatatt actgcttcaa
     7441 tttctacatc tgtatatttt aaagtttgtt taatttcatt taagtttagt tcttgtgaca
     7501 aacaaactgt acttgctccc tcttctttga agtggtttat actttcacta ttgaatacat
     7561 ttaagtagca atctatattt aagtcttttt tcttaaagta ttccatagag ccccagttac
     7621 caatttgaac cttattaatt ccttttatat tatccaatat ctcaaattgc ttgtactctt
     7681 tatttctaac aatccttggt gctgaataga ttaatttttt gtcagtgact actgtattat
     7741 agtggttctt attcaaagta tctattttac ttgtatttga caaagtatct atgttatttg
     7801 tatttgaacc cattagttct ttaggaatac ctaaagatga agaagtatct aaatccatag
     7861 aaatacttga atctatagaa atatttgaat ctatagaaat acttgagacc atttgtaaag
     7921 cttttgataa tgtatttata tcttcataat agattaaatc tacattataa tttaatactg
     7981 cttctaattg ctccaagttc ttaactttaa ctctaatctt aggagctgta tcaagttttt
     8041 tctgtttatt tactctattt ggctggtatt ttaatctatt cttcttaaat tttctatctt
     8101 ttatctttat tctttcttca tctaaaagct caatacaatc ccttctcatc tgatttaaca
     8161 cacttatagg catacttaca ccttcatcca ggtcaatttc tatctctttt aacttatatg
     8221 gtgtatttcc aagcttttga atttgtgttt ctattttttc tttacttaaa gccaccttta
     8281 tagctttttc aactattttg tctccttcta ccaccgattt atttccacaa aaatcactta
     8341 taaaaagctc tggcttttta cctagtttaa tactcacttt tgcacgtata ttagttttta
     8401 tgaactcttt atcttgctga aatgtagctt gtactctatc cattaattcg ctgtcagaag
     8461 tcttatatat aagctgattt ctctttgctt ctccaacaaa atctaattct atcaaatccc
     8521 ctttatgtgc tattgtagtt atctctccat cctttattat tcttccaata gtacctccac
     8581 ctaagttaat tccatcacct ttcttaagcg tattttctag cattactcta agtcttttgt
     8641 tttttctatt ataatctaaa acttttccaa tatatagccc ttgattatta ggtctattag
     8701 agttcattat atccttacca acttcaccta atatatatcc ttttgtaaac tttctattaa
     8761 atatggtata caaattattt attatttcct cagaaatact tatttttttc ttatctacaa
     8821 attcattaat cgcttctcta tatcctgaaa ccacagtagc tacatactct ggctttttca
     8881 ttctaccttc aatttttaaa gataatacac ctgcttcaat tattttatcc aaatcttcta
     8941 tagtacttaa atctctagga ctcaacaaat aatctccatt actttttact acttctcctg
     9001 tatttatatc tatcaactca tacttttgtc tacatggctg tgcacaccta cctctatttc
     9061 cagacctatt gcctatcata ctactcataa ggcattgacc cgagtaacat acacataaag
     9121 ctccatgtac aaaaacttct atatctacag ttgtattttc acaaatccat tttatctcat
     9181 caacatttaa ttctcttgct aaaacaactc tatcaaaccc tacactctga aggtatttta
     9241 catcttctaa ggaatgtgct accatttgag tgcttgcatg tagttcaaag tcaggtaata
     9301 gtttttttat aagtcttgcc attcctatat cctgtactat taaagcatca atatctatat
     9361 cgtataaaaa ttttacatac tctacaaaat cttctatttc atactgtttt atcaatgtat
     9421 ttacagtaac aaagacttgc actcccctta tatgagagta tttaacagcc tcaatcagct
     9481 catttctatc aaagttattt gcagatgctc tagcactaaa gtctttacca cctaaataca
     9541 cagcatttgc tccgttttga actgctgctt ttaatgaatc aaaagagcct actggtgcaa
     9601 gtaattctat ttttttcata tttttctcct ctatttatac ttgttaggtt aatataatta
     9661 tatctataac ttaaatattt gtattaaatt tatatatttc ttactatatt ttaaattctt
     9721 caatcaattt atatattagt tatttatatt tggttcattt gagttaacat atacattatt
     9781 tactatattt agtatatttc tattaactta tatattgatt actgtgtttt aaattcttca
     9841 attaatttat atactgctta ctaaatttaa aa
//
LOCUS       DAEMHZ010000145         9691 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000145 DAEMHZ010000000
VERSION     DAEMHZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.146"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(77..3439)
                     /locus_tag="KPK72_003218"
     CDS             complement(77..3439)
                     /locus_tag="KPK72_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490409.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDANAGNAGGDTNVGNPEGDANAGNAGGD
                     ANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLI
                     IDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQG
                     ILYLWFSDKFTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEE
                     TMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GNGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINIDTDGDG
                     KPDINIDIDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQT
                     GDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            3708..4280
                     /locus_tag="KPK72_003219"
     CDS             3708..4280
                     /locus_tag="KPK72_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG2490410.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKYQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(5294..5956)
                     /locus_tag="KPK72_003220"
     CDS             complement(5294..5956)
                     /locus_tag="KPK72_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490411.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIESHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(5973..8528)
                     /locus_tag="KPK72_003221"
     CDS             complement(5973..8528)
                     /locus_tag="KPK72_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2490412.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNLVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPNDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNIFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKGMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLEDPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(8768..8956)
                     /locus_tag="KPK72_003222"
     CDS             complement(8768..8956)
                     /locus_tag="KPK72_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG2490413.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSSDDIERITKEKYTK"
     gene            complement(8968..9147)
                     /locus_tag="KPK72_003223"
     CDS             complement(8968..9147)
                     /locus_tag="KPK72_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490414.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKPLELKLIKEFKLK"
     gene            complement(9144..9401)
                     /locus_tag="KPK72_003224"
     CDS             complement(9144..9401)
                     /locus_tag="KPK72_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490415.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYDH"
BASE COUNT         3078 a         1723 c         1046 g         3844 t
ORIGIN      
        1 ttatttacac aaatctattt atagtcacta tagtttatcc aataaaataa taaaataaat
       61 atgtacacaa taaatgttat ttacgtctta agtatttatt tgttagattt ttaattaaaa
      121 aaaacaagct aatgaacatt aatgctaaat ctagcattat attgctttga tctcctgttt
      181 gtacattttg acctacatta ctatctaaac tttcatctgt atctatattt atatctggtt
      241 ttccattccc atctgtatct atatttatat ccggttttcc atctccatct atatctatat
      301 ttatatctgg ttttccatct ccatctatat ctatatttat atctggtttt ccatctccat
      361 ctgtatctat atttatatct ggttttccat ctccatctgt gtctatattt atatctggtt
      421 ttccatctcc atctgtgtct atatttatat tcggttttcc atccccatct gtatctatat
      481 ctggtttgct atttcctcca ttatctggat ctggcttatt tagatttgga ttacctgtat
      541 cataacctcc tgagccagta tctacatttt tatcaggctt ccaataatca tctactccat
      601 cattatctgt atctattgct ggacgattat aatctgtatc agggtttcca tctccattgg
      661 tatctatatt tttatcaggt ttccaaatac catttccatt tccaccttct gtactaggct
      721 tccattcacc tgtattatca gtatctatgt taatatctgg cttccaatca ccactcgtat
      781 ctatattgat atctggcctt ccatcaccat cagtgtctat atttatatcc ggtattccat
      841 ccccatctac atctacattt gtcatatcta actttaactt cttttcatcc tcactacctt
      901 caatgtaatt tatatcatct ccaatatatt ttacagtaat agtttgttca gtagtaaaaa
      961 tatgatgatt gctatctaaa ggtctccatt caatagatgc agtactataa ttatgactct
     1021 cactaccctt attatgagtt tttaattcta ctggatcttt atattccttt ccattaatat
     1081 agaattgtac gtaacctctt ggagaggcac aatctggtcc ttctttctta tctggtgaaa
     1141 tagtaacatt taatgtaaac gtatctgttg gtttaaaaac atcagtttta gctttagact
     1201 cttctacaac taatgaagtt tctgtatcag caggaataat ttctgcctta taatatgctc
     1261 tatgccccca ataagcatcc ttaaaaggat atatactcga aaactgtgtt gatgtaatgc
     1321 ttaattgata tttcccagca tcaacttgtt ttgtaagatc atcacttcct aaatttatat
     1381 ctggttccat cgtttcctca tttaaacgtt gagaactaat agtcataaat tttttatctg
     1441 tgagtttttc acctttagga gtagttattg gaataccacc atcttttaat agccaatcta
     1501 aaaatgattt ttctacgtct tcttcaaatg gtaatccatc atatctttta ataatcaaat
     1561 atggtggagt atcatcaata gtaaattctt catattgctt taaaaattga gaacctgaac
     1621 ctatatcttt aatataaaaa tcatctggtt ttagaacttc tccagtaatt ttatctataa
     1681 caactaaatt aacagatact tcagatccag taaatttatc agagaaccat aagtaaagaa
     1741 tcccttgagc atctgtataa ctaggtgctc cataaggata aatttttcca tcaattagca
     1801 ttttaaaatc atcaactagt gtagtactaa ctttatcttg ccctaaatac gttagagata
     1861 ttttattcat aaaaactttt gttttctttt ctaaatcacc gtatgcaact gctccaccta
     1921 aacttttgaa tgttgttgct tttaatgaac cacctgttac tataacatct cccgttgtag
     1981 cacttaggtc aaaaatccaa tctcccactc ctgtcatata aggtaaaatc gttcctccag
     2041 ttacaagtat acgatatcct ctatttgttc ctggattaac acatccatcc ccaaatgcac
     2101 taccatgtgc acctccatat gaataggaaa gtccaccatt aattgtaata ttcccactat
     2161 aaccagaacc tgtgctagga tctactactg agggacttaa tgcattcttt gttgttgtat
     2221 agtatcctcc acctattcca gctccatggt atgcaccttt agcagttata gtaccaccat
     2281 tgattgtgat atctccacca ctaccattac cgcctccacc tattccagct ccatatcctg
     2341 tattacgact cgattcttga cctatagcag ttatagcacc accattaata ataatatttc
     2401 ctccactttc agtatttcca ccgccaatac ctgcagaata ataatttcca acaactttta
     2461 aacttcctct attattacta tctagtagtg taaggcgatc tccttttttt aacttgtctc
     2521 cattttctag agttacatct ctaggaattt ttccaccctc aggaactata aactcgcctt
     2581 caatatctct atttacaaca gaatcatcaa taattagagt agtacctttt cctgctctca
     2641 tacctggata atatacatca cctgactcta gcttgttttc actaccatct tgtaatataa
     2701 catgtgcata tgcaccacca tctttatctg gtgttaaagt aaatgggcta ttagatgcag
     2761 ttttaatatt tactccatca aatattaatt cagcttttac atctttaggt ataacaatcc
     2821 tatgtgttgt tggagtattt ggattattat tttgaattgt catttgtttt ccactaagaa
     2881 tagttaaaac gccacttgca aatttaaaat cgacatttat ttctccacca ctaattttaa
     2941 agtctcctac agatgtagga tcttctgcag tggcttcttc cacaactaat gcattcatat
     3001 ctccttctgc atttcctgca ttagaatctt cttctgcatt tcctgcatta gaatcttctt
     3061 ctgcatttcc tgcattagca tctccttctg catttcctgc attagcatct tcttctgcat
     3121 ttcctgcatt agcatcccct cctgcatttc ctgcattagc atctccttct ggatttccta
     3181 cattagtatc tcctcctgca tttcctgcat tagcatcccc tcctgcattt cctgcattag
     3241 catccccttc tgcatttcct gcattagcat cccctcctgc atttcctgca ttagtatctc
     3301 cttctgcatt tcctgcatta gtactttctt ctatagtatc tgcgaaagta tttgacgcat
     3361 taaatgatgc tactgatcca agtcccattg agatacttaa aatcattgat agtgctttct
     3421 ttttacattt ttgttgcata aattccccct ccccttaata atttttaata tacactgtat
     3481 acatccttat atacactcta ataattctta atatacactg tatacatcct tatatacact
     3541 ctaataattt ttaatatata cttaaaactt atatttgtca atgaatatac agtttttttc
     3601 aaccttatat attataagta ttcattattt gctaaataac tacaataaaa attttaatta
     3661 tataaaattt atttaatctt ttatataata atacaagggg taattaattg aaaaaactag
     3721 taaaaatatt aataaatatt atttgcatta ttacgctaat tttctctagc ctttcgattt
     3781 acataaaatt atctgaatat aaaaaagctg atgaagtata tacggaactc agagaaaaca
     3841 ctatcaataa tagcaaatat caagaacttt atgataaaaa taatgattat agattctggc
     3901 ttaaaataaa caacgtaaat atagactatc ctgtagtaca aggatataac aatgatttct
     3961 acctaactca tgatttttat aaaaattatc ttcctttagg tagtattttc atggattata
     4021 gaaataattt tgaaaatgat aagagtttaa tagtctatgg tcattatatg aaaaataaaa
     4081 ctatgtttgg tcagctagaa aactatactg atgaagtatt tttcaaagaa aataacttag
     4141 tagaaataaa ttataaagta caaacttata cttatgaaat tttttcagta tatactgctg
     4201 acttaattaa cagagattat ttaagtatac actttaatag taattatgag tttaaagatt
     4261 atttgaatta tataacctaa aaatcagtac acaaatctga tataaaggta gatatttcag
     4321 ataaaattat aactttgtat acttgtaact atgaatttaa agatgctcgt actatagttc
     4381 atgctaaatt aatttcaaaa aaagagactg ataagtaggt ttgtgtaaat gcagacttac
     4441 ctacagtctt ttttgaacgt aaaattggta agtatggaat aatatttata ttaaactttt
     4501 gtaggcaaag aaatgaaaaa tcaaagagaa ataattatta cagatttaga ttaccaagct
     4561 gaattaagaa agtgtaaaac tatggaggat gtagttggtg aagatagtat ataatagttt
     4621 aacttataaa ttatcttggt gatattaatg aagtttcaaa gtatatatta tttattgata
     4681 taaataaaat taaatctgtt gctaatagat gtacctttgt ttggagacaa actattgaag
     4741 aaatataact aaaatgttga agtagctacc tgactttatt ttgaaatgcg aacaagattt
     4801 tggcattaag ataatttaga aagaatcact ggtacacaag gatgtgaact tagaatgaat
     4861 agaagtattc aggcataagg agctttcacc caaataaaac atagtataaa ctttaaaaga
     4921 attctaatca gaggcaatca aaatgttctt tcatagttta tacctgtagt gttagcccat
     4981 agtatttcta agtttcacaa ataagataat cattaataaa tgtagtatgt atattcatcc
     5041 tctcaaaaat attttttaat cttacacttt gtaaattatt ctattttaaa atggtatttt
     5101 tataccttta tttcttatga ccgttttcaa gctcaaattt tgcataaata taaattaatg
     5161 aaaggcaagt aaaaaaagaa ctgtcgcaca gcaatttaaa attactcatt cgacagtcct
     5221 tatttcgtgg atttaaatag tggagaagaa tcacaagaat aatacccacg attaaacctt
     5281 tatacaatac attttatatg actttaccat cactaatagt tattatacaa tcagccatct
     5341 tagctatttc atcattatga gttatcataa ccaacgtctg cttgtattca tttacacaat
     5401 actttagtaa attcatcacc tcattagttg ttttactgtc taaatttcca gtaggttcat
     5461 ctgcaaaaat tattgatggc ttattggcaa gtgatcttgc tattgcaact ctttgttgtt
     5521 gaccacctga taattcatta ggaaatttat taatctgact ttctaatcct aacttagtta
     5581 ttaaatcatt aatataatta ttatctatct ttcctttagc aattaaaata ggaagaacta
     5641 tattatcata aacattgata acaggtatta gattatagtt ttggaatata aacccaaatt
     5701 ctttacttct aagattagat agcttttcat cacttaaagt atataaatct aaattatcca
     5761 taaatacatt acctaaagtt ggtttatcaa gccctgccat acagtgaagt aatgtgcttt
     5821 ttccagaacc actttgtcca attattgcac taaatttatg ggattctatc tctaaagtaa
     5881 ttccatcaac agcctttatt tcattttctc cttttccata tattttttta atatctataa
     5941 cttttaacat cttcatacat ttacctccca aattattcat tctcttttat accttcaata
     6001 atattcatac tttcaaaatc tctattactt acaactactg ctattaaaca tacaaataca
     6061 gatatagcaa agaatagtaa agattcctta attgggaatg taaaagacat aaaattctct
     6121 attccatagt ttatatacgc atcattattc caatttgtaa atttaactag atttataaat
     6181 gatggaatca tagctattat aaagctaaaa actgcatatg ctaatgcttc atatataagc
     6241 atttttttca tatttttaag actaacacct aatgctctaa gtgttgaaat ttcttttttt
     6301 ctaattagta aatttgttct tatagtacag aaaatattaa ttgatgctat tattaaaacc
     6361 agtgcaatta ttgctatttg tgatttaatg taacttttat atatactttc tgacatttca
     6421 tgataactta atctactatt aaatagagac cctggtatac ttcttgaaat tttctgtatc
     6481 tcattattaa caatctcatc atatggatct tctaaattta tgcctaattg agtgtatttt
     6541 tgttctcctg ttatgttttt agagtgaata ttacttgtta taatttctag atctggcata
     6601 aaatcgccat ctcccatagc aatccattca tcctttaaaa ttacagccac tcttaccttc
     6661 atccctttat aaacaattgt attattttct tcaacaggaa tttttacagt tattatgtct
     6721 cctatattta attctttgaa tataggcttt atagtatgat catcaactat atcataaaaa
     6781 taattatata ctgctacatt tggatatcca tcagtgtcct ttttaatatc atctaagcta
     6841 ccttcttcaa caaagtcatt taacttttca tctatataat catcatcata aagctttaaa
     6901 actaatttat cttccattat ctcttttttt tctactccat tataggttgt aaattctttg
     6961 tttatatcct tattattaaa agtaaacatt ccatttgtta tttgaatttt atgtatactt
     7021 ttaacaccct taattttttc tatattctct acatcactat ctgtgtattt tgatatttgc
     7081 gggtctgcat ttactccaaa ttgcattgtt atattatatc tattttgcat atcttcaatt
     7141 ttactgttga cctcttcttg catacttgag aatgtagtaa tgaatatata tcctcctaaa
     7201 gcaatagaca acatagatat agcagctcta accttatttc ttccgatatt tttaagtccc
     7261 ataaatccat aaaatccaaa tattttgctt actagtctgt ctattccaag tctacttctg
     7321 tgatttttaa ttttgtctgt atttattatt gattcaatag gtgatatctt gccgatttta
     7381 aaagttggta taatacaaga tataaaaaca gaaattattc caaccaatat agcttttata
     7441 actatttcat tatttatata taatttaggg ttaagaccag cttcactgta taaaacttcg
     7501 ttataattac taattcctat atatgaatat gtaaccccag ctactaaccc aagtagtata
     7561 cctattattg ttataattaa tgcttgatag attatcatac accttacgtt tcttttcttt
     7621 gcgcccatca gtctaagcag tcccatttct tttaccgatt gatttagtgt aatattgaaa
     7681 atattaaata tgaaaattat agatactgta gttattaaaa catttctttt aaaaatattc
     7741 ggatcattct tatcatcttc tacataacga agttccctta caagaggtac atttggtaga
     7801 aagctttttt gtcctaactt atattttttc ataagataat cacattcacc ttcaatattt
     7861 ctccatccac ttttaaaact taatatacta ttataactaa tcgcatcatt aggtataatg
     7921 ttgtttgttt catcattgcc ataagttact gcttcgtatt gccaaaccac atcatagtat
     7981 ccttttggtt tttccataac tcctactatt ttaaattctt tatttataga aaataattga
     8041 ttacttccgt tagtatcttt atagttcttt tttattgtta aatttaatgt agatcctact
     8101 ccttcactta aattcattgc ttccagtgct tttttctcta atactatttc attgttgttt
     8161 ttaggtagtc taccttctat tattttgttg tatctacttt ttagatactc ttcgtttgat
     8221 gaagttaata taaatgagtt tcctttttta tctgaaatac gccctagatt ttgtaccaaa
     8281 ttaccctttg atactttttc atcagtttct aattttttag catcatcaag tgtcaaatca
     8341 ctaaactcta catggtatcc actgtaaagt tcatgtattg ctactacatt gttttcattt
     8401 tgtgaatctt ttataacact aggtccaaat atcaacataa cgcctaacat aactcctatt
     8461 attaaaagta atgttctaag tttatttttc tttaaatatg ctagtgacat ttttaagtaa
     8521 atccccatat aatcctccta ttttactata aattatttat ccaactcatc tgttaagtag
     8581 attggtggat attttaaatc ttttaattct tcattatttt ttgaagctta ctaccatctt
     8641 cctcatgata aacttgcagt atcaccttat ttacatgtag tgcataataa tcaccattat
     8701 aaaaatatta gtatttataa cctggatagt ttttgtaaaa tgatttataa agtatcccat
     8761 cctaattcta ttttgtatac ttttcttttg taatacgctc tatatcatca cttgaaactc
     8821 cttgatatgt catatattta tctatatatt tttcagcttc attccatttt ctaaaatagt
     8881 tgttgtatat aataaatcct attatggcta taatgcttat aattaaagtt aataaaatag
     8941 ctttgtattt tctcatagtg aatcttccta cttcaattta aattctttaa taagttttaa
     9001 ttctaaaggc ttagttttat catcaaattt cttaacttga atttttatat ctcccaaatt
     9061 atcaacctct acatttactt cacctttatc aaatacttct tcaatttttc taaaaaattc
     9121 tttctcactt atttctatat gaatcaatga tcataaatgg ccattatgaa tttatcatct
     9181 tctgacagct tttcattata agaaaaatct ttaacacatt gaattgataa tatttccttc
     9241 gtatccttct tttccttagt tattgaataa tgtaaaactt ggctaggttg ttcttttgta
     9301 tactccaagt ttcccaaaga caaaacaaaa cttccatctg atttattttc agctacccta
     9361 agaccactat tttctattga ttctgatatt ttctttgaca agtcttcttg atactagaaa
     9421 tctgttcaat agtatatatt ttttcatttg tatctgaaac cttatttgtg catcctgtta
     9481 ttaaacctag tgttaagctt atagctataa ccatagttat tgtaattttt ttatcttcat
     9541 ataatcctcc ttatatattg aaaactcgtt atttctcata aaaaaaagac gctatctttt
     9601 tctattaaaa gataacatcc ctatatgttt agtatcaata tatgttcccc aactggtagg
     9661 ttaatcgacc caactatcag tattttgatt t
//
LOCUS       DAEMHZ010000146         9679 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000146 DAEMHZ010000000
VERSION     DAEMHZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.147"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..722
                     /locus_tag="KPK72_003225"
     CDS             15..722
                     /locus_tag="KPK72_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490416.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            769..2364
                     /locus_tag="KPK72_003226"
     CDS             769..2364
                     /locus_tag="KPK72_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490417.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNVYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            2467..3549
                     /locus_tag="KPK72_003227"
     CDS             2467..3549
                     /locus_tag="KPK72_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2490418.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLKGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            3698..3859
                     /locus_tag="KPK72_003228"
     CDS             3698..3859
                     /locus_tag="KPK72_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG2490419.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            4200..5468
                     /locus_tag="KPK72_003229"
     CDS             4200..5468
                     /locus_tag="KPK72_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2490420.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            5663..6451
                     /locus_tag="KPK72_003230"
     CDS             5663..6451
                     /locus_tag="KPK72_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2490421.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            6451..6900
                     /locus_tag="KPK72_003231"
     CDS             6451..6900
                     /locus_tag="KPK72_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2490422.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            6904..9168
                     /locus_tag="KPK72_003232"
     CDS             6904..9168
                     /locus_tag="KPK72_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2490423.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVTKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            9308..9619
                     /locus_tag="KPK72_003233"
     CDS             9308..9619
                     /locus_tag="KPK72_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490424.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         3715 a         1004 c         1698 g         3262 t
ORIGIN      
        1 tatggaggga aattatgtta aaagtaatca atgtatctaa atcatataaa aataaaaaag
       61 tagtaagtag tgtctcattt gatgtaaatg atggagagct ttttgggttt ataggacata
      121 atggagcagg taaaactact actattaaag caattgttgg aatacacgat tttgaagagg
      181 gagaggtact aataaattct aaatctataa aaaaacagcc tgttgaatgt aaaaaggaaa
      241 tggcatatat acctgataat ccagatttat acgagtcatt aactggtata caatacctta
      301 attttatagc tgacatattt gaagttgcca aagataaaag agaagagtta ataaaatatt
      361 attcaaataa gtttgaactc aataaagcat taggagattt aatatcttca tattcgcatg
      421 gtatgaaaca aaaattagct ataatttcgg ctttgataca cagcccaaaa atcttaattc
      481 ttgatgaacc ttttgtaggt cttgacccaa aagcttcctt tatattgaag gaaattatga
      541 gggagttttg tgacaaaggt ggttgtatat ttttctcaac acacgtttta gaagttgctg
      601 aaaaaatttg tgataagatt gccataataa aagatggaaa tataattgct tatggaacta
      661 cagaagaagt taagggaagt aattcacttg aaagtatctt tatggaatta atagagaaat
      721 aaaatgtttt ttatacaata tagtgtctaa agtgaggaat gataaaatat gagcaatcta
      781 agtatattaa taaaaaataa tataataaat gaatttaaat taaatgcgtt aaaaaaagct
      841 gatggtagag aaaaacaaaa aataatggga atgatgcttt cagttgttat aatagcgggt
      901 ctattaattt tttatgtaac gagcttaagt ctctctttag ttgatatctt aaagcaaata
      961 aatcaactgg aaatgctact gatatttggt tttggattat caacattaac aactattgtt
     1021 acatcattat ataaaacatc atcatatcta tttcaagcaa aagatttaga ccttttgact
     1081 agtttaccta taaaagaatc tactgtacta gcaagtaaga tattaatgtt ggttggagca
     1141 aactatttat tttcttcaat atgtatgatt atacctgcag tggtttactt tttaaattct
     1201 gatgtaagta taacttattt cccttatttg atattgctat ttttagcttt accattatta
     1261 ccgatagttg cttcatcaat aatttttcta tttatagggc agatatcatc aagaataaaa
     1321 tacaaaaatt tagtattaat cataggtagc attatattga tgttgcttta taccctactt
     1381 atgtcaagac ttaattctat gtcagttgaa atgctaaaga atagtgtgtc tatttcagag
     1441 acgattagaa aagtatatcc tcctatatat tattttgttg atgctcttaa aacaggaagt
     1501 atagtttcat ttttagcatt tatagccatc tcaataatac cattttttat gtttgtaact
     1561 ctgtttgcaa aaaaatttaa agatataaat gctaagatga gtgaaggata taagtcaaaa
     1621 aattatgaat ttaagggtca aaaaacttca agaccttcaa aagctttatt aaaaaaagaa
     1681 tttaagagat atacttcttc gtttatatac tttttaaatt catctgcagg tttgttattt
     1741 ctaatagtag gtacaatttt aatattatta tttggagcag ataaagtggg tgagttactg
     1801 aaattgaata tagattttaa tcttataaaa atacaattat tgggagtatt cttctttata
     1861 atagtaatga attgtacaac atactgttca atttctcttg aaggaaaaaa cttatggata
     1921 ctgaaatctt ctccaataga tgaaagagaa atattctggt caaaaatatt gatgaatttt
     1981 ttattgaatg caccactatc tattttatgt ctaatattag tatctataag attagacttt
     2041 gaaattcagt ttattattgt atcaatctgt ttgattgtaa gtttttcaat gtttgtagca
     2101 ttgatgggat tattatcaaa tttattatac ccaaaccttg attggaaaaa tgaggtggct
     2161 gtagtaaaaa gaagtgcaag tatgatagtt acaatgttag tatcattagc ttatgtaatt
     2221 atacttggag gtatatatgt attacttgga atttcgtttt taaatgtata tattattttg
     2281 gcaagtatac ttactttgat attagatttt atgatatgga gaattatttc tactaaagga
     2341 gtaaaaatat ttaaaaattt ataagtgtat ttatgtcaaa taaagactta tcttttttag
     2401 tcttgcaaat aataggttaa gtagtatact atgtatagtg atatgaaaaa atttaggaga
     2461 aaataaatga gaaaattatc caaaagagaa aaagatttta ttcaatatgg atttttatta
     2521 tttttgatat gtattactac ttatttagta ttaactactt tagatataaa actaatacct
     2581 aaaattataa ggctagtaaa taagacttat atatttcttg gcatattgac aattatagta
     2641 tatatgataa ttgaatctat tgtaactcat ttgataataa atgcagttca aaaaacaaaa
     2701 gtaaaattta ttggtattaa aatggctaca atgggatttt actataactt agttactcca
     2761 tttgcatctg ggagtcaacc aatgcaaata tatgcactta caaaatatga tgtaagcctt
     2821 agtaaatcag tggctatagt tactaataaa actgtagtat ttcaaagtac agttactgta
     2881 ttttgtggaa ttttaatttt gctaaataag tcattactga tggagcaagt acattctgtg
     2941 aaagtcttga taacttttgg aatgattatg aatgtaagta tgttgttagg tggactacta
     3001 atagtattta gtccaaaaca agtaaaaatg atagcagaag ttttgattaa ttttttatct
     3061 aaatttaaaa catttaaatt tttagattca aaacgtgata gaataaatca ttatattgat
     3121 gattataatt attctataaa aatatttatt aaaaatgtaa gaatcttagt tttaagtata
     3181 ttacttactg tgttacaatt aatcgcatat ttcagtatag tttattgtgt gtataaagcc
     3241 tcaaatttaa aaggagtttc atatgtacat ataatgacat tacaggtctt tttatatatg
     3301 gctatatcac caattccaac accaggaaat gttggagcta atgaggttac attttttact
     3361 atgttctcca atataattcc aaaagaatta ttggggtact ctgtactctt atatagtggg
     3421 tttgtatact attttatatt aatagtttca ggtttattta catttggaac gcattataca
     3481 ttaaattctt ggaaaaagga agataaagta aattctaatt tagataaggt tattgaaaaa
     3541 ttaaattaat aaaataatta gggaaattct atatataaat atggtgttga ataagctata
     3601 ttatattgag atatttccct tttttgatta aaattataga aatttgaaat aataagtaat
     3661 aaacagaaaa agtgatattg ttttgaaagg agaaactatg aaacaagata aacaaaatga
     3721 aaaacaaagt gagatttcta agataaaaca aagcaatgat aataaacttg aaatagctca
     3781 agaattacat gtaagtaaaa atatagggaa tatgcttgga tatagaatag ataagaaaaa
     3841 aatgaataga ccaagttaat gaaaatgttg aaaatggtac attttaaaaa atatatataa
     3901 tgaaatgttt tattgttaaa tttaaactta ttttaatggg ggaaaaagtc gaaaaatatc
     3961 taaaaagata tggatataaa aaatattaat atatatatac tataaacttg acaaaatcaa
     4021 ccttttaata actagcaaaa aatagcaaaa ttatataagc gttatttaat tgtcaaaaat
     4081 gaaagctaaa ttgcatgtcc taaaaaaaat gattgataac atttatttgt gtgctataat
     4141 tatatagtaa aacaataagt atattgcata atgtatgcaa atcgggagga gagacggaca
     4201 tggaagcatc tatacacaca attgccagca ataatttatt gataattttt gctattgtaa
     4261 gtataactgg aataatttgt agtaagctta gtgaaataat aaaggttcca gatgtagtgt
     4321 tatatttact tgtaggtatt ttaataggac catcattttt aaaatttata gatattcgtg
     4381 gatttcaaat agaaaatcaa ctaatactta catttggttc tgcatttatt ttatatttag
     4441 gtggaaaaga aataagttta aaagtattga gaaatgtgaa gatatctgta ttcttattag
     4501 ctactttagg agttgtgata tctgctttta taatgcaaca agtaatagga tttacttttg
     4561 ggataagtgc aatgactgct ttactagcag gaactataat agcatcaaca gacccagcca
     4621 ctcttgtacc tatatttaat caggtaaaga taaaggatag ggtaaaacaa actgtaataa
     4681 gtgaatcagc ttttaatgat gcaacaggag ctatactaac atcagcagtg ttggctgtaa
     4741 ttttgtcaaa taaattttca ttaggtgaaa atatttatga actggggaca atggttattg
     4801 tcggagtttt agttggttta attacaggaa tactgctttt gaaattagtt aatgataaac
     4861 cttatggaat atttaaagat tttgcaccaa taatatctgt tatttcagtt gtagttgcat
     4921 atgaattatc tacaaaatta ggtggaagtg gatatatgtc ttgctttatt gtaggtataa
     4981 taactggtaa caagaaaaac tttaaaattt ggttaagtca aaaatcttat gatgcagatt
     5041 tttatgttgc tgaaacttta ggtactattt gtcgtatggc aatatttata atattgggta
     5101 gccaagttaa tttggctcag ttaagtaaat attttatgcc atctgtaatt acagtattag
     5161 tattaatgtt tatagcaaga ccactttgcg ttttagtttg tacacttgta gacagggaag
     5221 caaaatggag taaaagtgaa atattattta tgatgtgggt aagggaaaca ggcgtaatac
     5281 cagcagcact ttgtggtatt atttcagcaa tgaaagttcc tggatacgaa gtgatttcat
     5341 ctgtagtatt catgactata ctaattacat tagtaataca aggaagtact acaaaattag
     5401 tagctaaaaa acttggatta ttagaagaag aagtgataaa tataagtgaa aaagtatcaa
     5461 ctttttaaag ttttgcttta aaatagtacc tgttaatggc tattttaata ttgtctaatg
     5521 atgattattg aaatagtcat ttttttatgt ataagtaatt tacagaattt acatattaga
     5581 catatttatt atcaaaattt gttgaagcta tgatataatt agttgtattt aaaataagaa
     5641 tagcaaataa gggaggaaat ttatgtttac ggttatggat attgtacagc cagatacagt
     5701 agaagaagcg tactcaattt taaataaaag aaaaactaac caagtaattg gtggaagtgc
     5761 ttttttaaga atggggaaaa aaagaatagg aacaggaata gaattatcta aattaaattt
     5821 agattatata aaagagtatg aagattacgt tgagattggt tctatgacta cttttagaac
     5881 attagaaaca agttccataa taaaaaataa ttttggaaga ataatagaag attcagttaa
     5941 aaatataata ggagttcagt ttagaaatgt agtaacagtt ggtgcaacag tattttctaa
     6001 atatggtttt tctgacttga tagtagcatt actttcatta gatacagaag tagaattata
     6061 taatattggg agaattagtc ttgaagagtt tctaaataga gattatgaga aagatttact
     6121 tattaagata tacataaaga agacaaataa gaatgcttct tataaatcac ttagaaatgc
     6181 aaagagtgat tatccaattc taaatgtatc tgtatcaaaa catatggaac aatttaagtt
     6241 atgtgtagga gcaagaccgc agaaagctac gattgcaaaa caagctagtg aatttctatc
     6301 taacaatgaa atcaatgaaa ttaatataga taaagcagta gaaatagcat cagaagaact
     6361 aacttttggt tctaacatga gagcatctag ggagtataga aaagctatga gtaaagtact
     6421 tttaaaaaga gctataatgg aggttattta atgttagtaa agattaatat aaatgggaag
     6481 aaaaaaagtg ttgatataga accagaagaa tatctggttg atactttaag aaaagttggt
     6541 aatttaagtg tgaaaagagg ttgtgacaca ggctgttgtg gtttatgtac agttttaatt
     6601 gacaagaaac ctacattatc ctgtgcaaca ctgacattga gagctttaga taaagaaata
     6661 actacaattg aaggtttaga aaatgaagtt aaagaattta gtgaagtttt ggtaaaagaa
     6721 ggagctgagc aatgtggttt ctgttctcct ggctttatac ttactgtact tgcaatgaag
     6781 gatgagcttg aaaatcctac agatgatgaa ataaagcatt atcttacagg aaatctatgt
     6841 agatgtacag ggtatatggg acagttaaga gctatcaaaa cttatctgga ggtaaaatag
     6901 aacatgaata tagttggaaa aggaatcaag aaaatagatg gaatgagtat cacaacagga
     6961 aagcctgtat atacagatga tttatcagca aaagatgctt tggtggtaaa gatacttaga
     7021 agtcctcatg cttatgcaaa aataaaagat atagatacat caaaggcaga aatggttgat
     7081 ggtgttgaat gtgttttaac atataaagat gtaacaaaat ctcgatttac acttgctggt
     7141 caaacatatc cagagccatc tccatatgat agattaattc ttgaagatgt tgtaagatat
     7201 gtaggtgacg aagtagctat agtggcagct atagatgaaa aaactgcaat taaagcaatg
     7261 aatcttatca aagtaaagta tgaagtactt acacctgttt tggattttga agaggcaata
     7321 ggaaataaaa ctatggtaca tgatgaagat agacattgta actttgatat aggtatgcaa
     7381 agagaaaaaa atatagtatc ccatcatgat tatacaaaag gtgatgtaga aaaagtatta
     7441 agtaattgtg atgtagtgat tgaagaaact tattatactc aagctcaagc acatgcaatg
     7501 atggagacgt atagggcata caattattta gaccatatgg gaagattggt tgtagtaagt
     7561 tcaactcaaa taccatttca tgtaaaaaga catttatctc gtgcactaga aataccagct
     7621 agcaaaatta gggttataaa acctagaatt ggtggcggat ttggtggtaa acaaactgcg
     7681 tgtgttgaga ttttttctgc aattgttact ttaaaaacag ggaaaccagc taaaataatt
     7741 tatgatagaa aagaaacttt taattgttca acaagtagac atgcaatgag attaaaagtt
     7801 agactaggtg ctacaaatga tggcataata aaggcaatag atataaaagc actttctgat
     7861 acaggagcat atggagagca tgcttctaca actattggtt tggttggaga aaaaacttta
     7921 ccaatgtata ataaagttga agcaacaaga tttattgcag atgtggtata tactaataag
     7981 atgccagcag gagcacttag agggtatgga gcatcacaag gatgttttgc tgttgaatca
     8041 actataaata tgcttgctaa taaattaaaa atggacccaa cagatttaag gttaaaaaat
     8101 gtggtaacag aaggtgaaac tacatttgca tatggtaaaa ctttaaatag tgtcgattta
     8161 ataaaatgta taaataaagg caaagaactt attggatgga atgaaaaata tccttgtaaa
     8221 catcttgata atggaaaaat tagaagtgtg ggtatggcaa ttactatgca aggttctggt
     8281 atacaaggtc ttgataagtc ttcagctgaa ataaaattga atgactgtgg agattatact
     8341 cttatgatag gttctactga tatgggtaca ggaagtgata cgattttagc tcaaatggct
     8401 gctgaggttc ttgatacaag cattgaaaat ataacagtta tatcagctga tactgactta
     8461 gttccatatg acccaggttc atatgcttca agtactactt atgtaacagg tatggcagta
     8521 gttaaggcag ctcaagactt aagagaaaaa ataatttctg ctggagcaac taaactaggt
     8581 gttgagaagg atgaagtaga atttgatggt ataaaattaa ctagtaaaaa aggtagcact
     8641 ttattattcg atatagcagt tgattctaca attggtccag atactaatca attaataggt
     8701 cttgcttctc atggtagccc agtatctcct ccacctttta tagcaggttt tgcagaaact
     8761 gaaattgaca cagaaactgg aaaagttgat ttagtaaatt atgtagctgt agtagattgt
     8821 ggaacggtta taaataaaaa tctagctaag atacaagttg aaggtggaat tgttcaaggg
     8881 attggaatgg cattatttga agaagttaga tacacagaaa aaggaagcat ggacacaaat
     8941 acatttatgc aatacaagat tccagctaga tgtgatattg gaaatgttaa agtagatttt
     9001 gttgaaactt atgaaccaac tggaccattt ggtgctaaat ctgttggaga agttgttata
     9061 aatacacctt taccagcaat acaagaagct gtgtttaatg gaattggtgt aagaataaat
     9121 acactcccta ttacacctga aaaagttttt atggaaatga ataaataatt atttatgaaa
     9181 atttagacta gttttacatg ttaaacaacc agttatgtca tacctgttga aagatattta
     9241 ataagtagtt attataatat tatgaatttt acttataaaa tacaaaatta aataagggga
     9301 tgatgttttg gctggtaatc taaataatat gagagcagta aataatttta gaggagataa
     9361 gaacatttta gaatgtttag tcagctttga gggtcgttca ataagtcaga gaaaagtaag
     9421 ggtatttttt aaagaaaaac aaaatcaaat agaaattgat tttgcagaag aggaaatttc
     9481 taaattggtt gaaaatgttg ttttaaatac atcatatcaa gaaatgttat atgatgaaat
     9541 agagaaacaa ctggaaattg attgtatagg tacttggatg atattatcta aattaaaaga
     9601 tggtagtaga gttcactgat tttaaaataa attattagag cctaatttta aggctctttt
     9661 ttgctgtttt taattaaca
//
LOCUS       DAEMHZ010000147         9185 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000147 DAEMHZ010000000
VERSION     DAEMHZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.148"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            280..4407
                     /locus_tag="KPK72_003234"
     CDS             280..4407
                     /locus_tag="KPK72_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG2490425.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGIFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPIFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTESCVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCKDCCCNCCKNDFWC"
     gene            4864..5211
                     /locus_tag="KPK72_003235"
     CDS             4864..5211
                     /locus_tag="KPK72_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2490426.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(5361..6410)
                     /locus_tag="KPK72_003236"
     CDS             complement(5361..6410)
                     /locus_tag="KPK72_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2490427.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            6796..7530
                     /locus_tag="KPK72_003237"
     CDS             6796..7530
                     /locus_tag="KPK72_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2490428.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(7673..8164)
                     /locus_tag="KPK72_003238"
     CDS             complement(7673..8164)
                     /locus_tag="KPK72_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2490429.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            8513..8896
                     /locus_tag="KPK72_003239"
     CDS             8513..8896
                     /locus_tag="KPK72_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490430.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
BASE COUNT         3454 a         1250 c         1421 g         3060 t
ORIGIN      
        1 gttattataa atacattgaa aatacttgat tattgaaaaa tggcataaaa taatacattt
       61 tatgccattt actattattt gatagttttt aacttatgac ataagatatt ttttaacaca
      121 tacaataaaa tataaaaaaa ttgatattgc aaataaaaaa aatacatatt agatgttata
      181 gaaaaaaagc gcacttatag gcttaaaatg tcacataaaa gagttttaaa gaataatata
      241 ttatgaacac aaattttgta atcacaaagg agtgataata tggcaacagt atcaggacaa
      301 atagtttttg atagaaatag aagtgctacc attgattctg gagactcagg aatagccaat
      361 ataccagttg tattacaaga tatagcaact ggagttagac ttgtggttct cacagatgca
      421 tcaggaaatt atgtttttac taatgttcca aatggtaact atagaattgt agaagctttt
      481 ggaactacag gaggagtacc aacaccaggg aattttgcaa cagctatagt aggtccagtt
      541 ccaattgcag aaatgccacc tattagttct gtgacaaatc ctcctattgg ttcaacaaac
      601 ttagatggta caacacctaa cacactatta ataacagtag caggttcaga tataacaaat
      661 caaaatatat taaatggacc tgtgatttat acacctatag agaatatatt ggattcttgc
      721 gtatctgtat ctaatataaa ccttattaca gatgcagata acggaatatt cggttttttt
      781 ccaccaggta caccaagtaa tacaggacct gaaatagaac cttacccaaa tgtgacgcca
      841 gattttacgt atgttgtacc agacccaact aagtttacac ctttagatgg agaatataca
      901 gtacaaaata ttatgacaaa tgcaatgagt aatgttatag gagcttggtg gcgtattgct
      961 gaccatacta caggaaatga aacaggaaga atgatggtgg taaatggttt taatcctgga
     1021 gctgtatttt ttaaatcaac agtacaagtt acacctaaca ctaattattt atttagcact
     1081 tggattttaa acctttttaa ggtaacagga tatccaccac cacagctagg tgtaagaata
     1141 tttgaccaaa ataataatat actatatcaa gcaacattag gagctcaaat tccagttaat
     1201 acaaacgcac cagaatggaa agaagtaggt actgtgatta attctcaaaa taataatcaa
     1261 cttacagtag aattttttag tgaaggagaa agcgccattg gaaatgacta tgctatagat
     1321 gatgtttctt taagagaaat agctgttcca atttttactc caataaaaac aagtaacaca
     1381 gaaactgcta atgtaggtga tataatcaca tatacagtta cattagataa tacttgtgat
     1441 agccctttaa ctaatgtatt ttttagggat ttaattccaa atggtttaga gtttatacct
     1501 ggaactgtaa cggttgatgg agtatctgta ccaggagtag accctaatac aggtttccca
     1561 cttccagata ttggaggagg aacaggggtt gaagtaactt ttgatgttgt ggttgaaagt
     1621 attcctaacc caaatcctac taataatata gctaatatag attactctta tacacctgta
     1681 gaaggcggta taccaaatga tttttcagta gattctaatc cagttcctgt tgaagtaatc
     1741 tcagcagata ttgaagttac aaaattatct gaacctacta tagtgaatcc aggagaggaa
     1801 ttaatataca ctataaaagt agtaaataat ggaccattcc catctgaaaa tgtggtttta
     1861 acagatgatg taccagcttc tattgtaaat ccagagtatt cattagatgg aggagtaact
     1921 tttcaaccat ggactggaag tttaaatata gggacattag aagtaggaga gacaagagtt
     1981 attataatta ggggtattgt aaatccatca acagtaggaa ttataacaaa tacagctgta
     2041 gtatcttcaa ctacagcaga cccaaacctt aataataata catctacaat agagacagaa
     2101 gtgaatttat tagcagatat tttagtaatg aaaacagcag aacctaattc agctgttcca
     2161 ggtactttat taagatatac tattcaagta gaaaacttag gaccagcaaa tgctgaaaat
     2221 gtaatattaa atgatgatat tccagcttct attataaatc cagaatattc attggatgga
     2281 ggagcaagtt ttcaaccatg gaatggaagt cttaatatag gaactttaaa ctctggaatt
     2341 tcattaacag ttttaattca aggaacagtt agtttaaatt cttcagaata tatagtaaat
     2401 acagcaacag tttcttcaac tacacctgac ccagatttaa gcaacaatat atctaccatt
     2461 attacaccag ttaacccaca agcaggtatt tctattataa aagtagctga cgaggatgta
     2521 gctgttccag gagaagaatt tgtatataca attgaaattt ttaatgaagg accatctaat
     2581 gctacgaatg tagttttaac agatgatata ccagatgtta ttttaaatcc agaatattca
     2641 ttggatggag gagcaacttt ccaaccatgg actggaagtc ttaatatagg tacagttgca
     2701 ccagggcaat taataagaat tatcataaag ggattagtaa gttcaactgc tacaggagat
     2761 attactaata cagcagaagt aagtgcagat gtaccagaac cagttacgga ttcatcaaca
     2821 gtgacaactc ctatagttcc atcagcagat atagaagtaa taaaaactag caatatggat
     2881 acagcagttc caggagaaac tttcagttat actataacag ttacaaattt aggaccatct
     2941 gctgcacaaa gtatcgtttt gacagatgat ataccagatg ttattttaaa tccagagtat
     3001 tcattggatg gaggagtaac tttccaacca tggaatggaa atcttagtat aggcactttg
     3061 gatgctggag aaataagaag tattataata agaggaacag tgagccagac agcagtaggt
     3121 acaattataa atacagcaac aataagttca actacacctg acccaaatcc agataataat
     3181 acttcaacag atgaaacaga tataagtcca ttagcagata tttctgtgat aaaaggcaac
     3241 gaaccagttg ctataccagg gggaagattt atttatggaa ttgaaattgc aaatgcagga
     3301 ccatcttttg cagagaacgt aactttaaca gataatatac cagcttctat tttaaatcca
     3361 gagtactcaa tagataatgg agtgactttc caaccatgga atggaagcct taatataggt
     3421 actttagatg ctggagagat aagaaatatc ataataaggg gaacagtaag tcaaacagca
     3481 ataggcacaa ttataaatac agcaacagta agttcaacta cacctgaccc aaatctaaat
     3541 aacaatactt caacatcaga agcagaggta agttcatcag cagatatttc tgtagtaaaa
     3601 cgaagcaatc agattgttgt tgtaccagga gatgtactag actatacaat tgaggtgaga
     3661 aatgcaggac cgtctactgc tcagaacgta actttaacag ataatatacc agcttctatt
     3721 ttaagtccag aatattcaat agataatgga gtgacttttc aaccatggaa tggaagtctt
     3781 agtataggca ctttagatgc tggagagata agaaatatta taataagggg aatagtaagt
     3841 caaacagcaa taggtacaat tataaataca gcaacagtaa gttcaactac acctgaccca
     3901 aatccagata ataatacttc aacatcagaa atagatataa gttcatcagc agatatttct
     3961 gtaataaaat tagcaaataa aacagaatca tgtgtaggag agcaaataga ttttgtaata
     4021 gtagtatcaa atgcaggacc agaaaatgct caaaatgtta ctttaataga caatgtttca
     4081 gataagctta agaaagcagt attttcactt gatagaggtg taagttttca accatggact
     4141 ggaagtctta atataggtac actaccagct ggaactttaa gagtgattct attaagagga
     4201 attataaaat caacatgtct tgatagatta acaaatattg cagaagttac ttcaacaaca
     4261 ccagattcaa accttaacaa taatatatca agagtgcaag tagaaataaa acaatgttgt
     4321 tgtaatgatt gctgttgtaa caactgtagt aattgttgtt gtaaagattg ttgctgtaat
     4381 tgttgtaaaa atgacttttg gtgttgattt tataaaataa ttttgtatac cttaaattaa
     4441 actgaaataa aaaataaatt cacatatgag taaaatattt tataaatatt aataaatttt
     4501 ctatctttga tatggaccac attcgtaata ctagttatta aatgaagtgg tcctttttaa
     4561 ttcattaaaa ttatttattc taattaaatt ttaaatgtat taaataattt taatttttta
     4621 taaaaaatat tgatatgtta tttatattaa agtttagata tctaaaaata cttttagaat
     4681 ttattatatt atgttaattt tttatatctt ataaggattg tatttttcta actataaaaa
     4741 tagtattata cttatattta aaaataaaag aattttattc ctattgtgta aagtgagaat
     4801 actattttct tatattggta taataatagt aaatctataa tatagaaaag aggtatttct
     4861 agtatgcatg aaaataaaaa gaatctatta gacggaagct tagctcaata taatgataca
     4921 gcagttccaa aaataccagt atttgctggt aacgatataa cttcagaagt ttattatttt
     4981 aaacctaatc aagttcttaa tgctcataga caccctaatg gtgaacaaat atttgtgttc
     5041 ctaaaaggag aaggtaaaat gaaacttggt gagcatgaat gtgatgtaaa aaacggagat
     5101 actgtatttg taccaacagg agagtggcat gaaattacta atggaagcaa tgaagaaatg
     5161 gtagccgttc aaattacaaa aattaatgct ggagcagaat acagaggata gatatataag
     5221 tacagttagt actttaatat ctattttatt atataaactt taatctcaaa tataaaaaga
     5281 gtcttttaaa aggctctttt tatatttttg ttaattttca ttataaaaac aagaaaatat
     5341 ctagctaaaa ctaatatatt ttatttaata ttttttatag cattgtttgc aaactcagta
     5401 tttacgatat ctttataagg tgcttcctta tctagttctt tagcttcttt cataacagtc
     5461 atcaatttct tcaaaccttc ctcttttaat acaggatttt gagaccatgc atctattgat
     5521 ttatatctat ctacaactgt tgttaaatct tctaaagata tatcttcaaa gaaaggttgc
     5581 atagccttag ctacttcttt gctagatgct tgttgaatcc ataattgacc tttataaaca
     5641 gcatttgtaa atctttgaat taaatcttta ttgtttttca tgtatgatct cgtagtagag
     5701 tatgctgtaa aaggtatttc tccagaatct tttcctatag atgtaactat atatccacta
     5761 ccttcttttt ccattgctgt tgctgtaggt tcaaatgctg ttgtaaagtc accctctcca
     5821 cctgcaaaag ctccagccat tacaccaaat tgtacatcag ttcttacatt aacattgcct
     5881 gcttttagat tttctccaat agtaagacct ttttctttta gaacatattc aaaagtcatt
     5941 agtggaactc caccttttct accacctaat atttcggttc cttttaaatc attgtaagag
     6001 aaattaggca tttttttcct agcaactaga aaactaccat ctctttttgt caattgagca
     6061 aagttcactg cataatcgtt tttgttttga ttatatacat atatagatgc ttcaggaccc
     6121 attaaaccta cgtttgcttc acctgaaatc aaagctgcca tagttttatc tgcaccttgg
     6181 gtatttataa ggtcaatctt tataccttca tcttcaaaga atcctttcgt aatagcagca
     6241 tactgtggag cataaaaaac agagtgagta acttctgcta tagttacttt atccagattt
     6301 ttagacgaag aagattcacc ttttttgtca ttagagttaa aatcacatcc agttagacat
     6361 attgtactta caatgataga gcttgctatt gcgactattt ttttaaacat aataaatacc
     6421 tcccataaga catacgaata gtatatagta ttaaaaattt attgtagttg ttacaaaaat
     6481 gtatctatgc ttataatatt tctacaaaaa cttaataata attttcagtt ttatgtttag
     6541 tttttttagt aatgtgtaat attggataaa ttatattgat tatttataaa ctaagtaata
     6601 aaattaaaat aatatcactt ttttgtctat tatatataag gaaaaatttt ttaataatat
     6661 aaatcatatt catttatatt attaaaaaat ttgtttatag aaacttttat tattttatat
     6721 aaaagtgata aaaacttata ttttgtattt aatagagtgt tgctaagtga atatatttaa
     6781 aatggaggaa actttatgaa acaaaataaa tatgatgatg atagattttt taataaatac
     6841 agtaaaatgg aacgttctac aaatggatta gcaggtgctg gagagtggca tgttttaaaa
     6901 aaaatgttac ctgatttcaa aggaaaaaga gttttagatt taggatgtgg atttggatgg
     6961 cattgtcaat atgcagttga gaatggagca atttctgctg ttggagttga tatttcagaa
     7021 aaaatgttaa aagaagctag aaacaaaacg caacttgata atataaatta catatgtatg
     7081 cctattgaag atattaattt tccaaaagat agttttgatg tagttattag ttccttggca
     7141 tttcattata ttcaatcatt tgaagatata tgtaagaata taagtaattg tctttcaaat
     7201 aaaggtaatt ttgtattttc tgttgaacat cctatattta ctgcatatgg aagtcaagag
     7261 tggtactgtg ataaagatgg cactaatcta cactggccag tagatagata ttttgaagaa
     7321 ggagtacgta agtcaaattt tctaggagaa gaggttataa aatatcataa aacattaaca
     7381 acttatttaa atacgttaat gaaaacagga tttgaaatat tagaaattat tgaacctcaa
     7441 ccagaagaaa aactgttaga tacaattcct gatatgaaag atgagctacg tagacctatg
     7501 atgttacttg tatctgcaaa aaaaagataa gtaaatataa ataatcatta ttgtagaaaa
     7561 aaagttgtag aaaataatta cactaatgga gatgattata ccagtggtat taattacatc
     7621 agtagtaagg aggcaaaagc attcgttgct tttgccattt tttaagttgc aattatactt
     7681 ctacgtagta tatttcgttt ccattttctt ctataagctt tttcatatct tcaaatttta
     7741 aaaatacagt agcagtatta tcatttggat gtatacctat tttatccatt tttaataaat
     7801 ccttatcaaa tacaactttt acaacagcct ctttatcatt taatatacca aaaggagtca
     7861 cagctccttt agtaagtttc aagtattttt gaagtctttc ttctgatgca aaactaagag
     7921 gtttactatc aatttgttga cgaatatctt ttaaatctgc ttttttgtct ttacctaata
     7981 caactaaaaa atgttctttt cctttagcat cacgaagaaa taggtttttt acaacaaatt
     8041 ccatatgtaa tgtttttaat tcttccattt cttcaattgt ataaactggt ggatgattta
     8101 cagcctcata aggtatgttt aactcatcta attttttata tacttcctgt tgttgattgt
     8161 tcatgatttc ctcccattag aaatttattt tatatataaa taatattatc acagaaaact
     8221 ttaaaagaaa acgttttata taatatagac aatagattat atttttataa agaagcaaga
     8281 atactcaata aaacttaaag aaattaaaca atttctaaaa ataccttgaa aatagaacct
     8341 tatttgccat ttataaatta tataaaaagg gattatataa agtcatatat gaaaaaacta
     8401 tattatacta tcttttcttt ttggaataaa ctatacttta aatatagata tttaatttaa
     8461 ataaatttta cagtattaat agtaaattat cttagaaagg aatgtgcttt atatgtctac
     8521 agacaaagtt gttggaatca tagatgaaga aacggcagaa cttgcaggaa ttgaatatac
     8581 tggtaaaatt tatgcttctt ctggtgttat aaaacatata aagaaaaaac atagatgtca
     8641 actgagtaag gatattttta atgatataat agacactata aaaatggtat taaagtcacc
     8701 tgaatatata ggaagtcatc ctaaaaaacc tggaaaaagt gtggagttta taaaaaaaat
     8761 tgatgattat attttagtag ctacagaatt agatacaaaa aaaggatatt tatatgtatc
     8821 ttcaatgtat gctataaaag aagcaaaatt agaaagtaga gtaaacagtg gaagagtaaa
     8881 acaatatgat ggttaacata atattattaa ttttccctaa aatattgaaa aatattatta
     8941 tataagttat aatattaata tacacgtaga tatttattag ttagatttga ggttggaaaa
     9001 ggcacccaat acgctactat agagtagtta atagagatgc aggatacgcc gccctgccaa
     9061 atctaattaa attaaaaaga gaggattaat tcctctctta ttgttttttt gagatactaa
     9121 tttaatctat aatagaaaga ttgaaggtcg tttatacaaa aaaattttca gttatataca
     9181 ttaaa
//
LOCUS       DAEMHZ010000148         8855 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000148 DAEMHZ010000000
VERSION     DAEMHZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.149"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..393
                     /locus_tag="KPK72_003240"
     CDS             <1..393
                     /locus_tag="KPK72_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490431.1"
                     /translation="IKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASII
                     IGITLFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGN
                     LNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            380..1135
                     /locus_tag="KPK72_003241"
     CDS             380..1135
                     /locus_tag="KPK72_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2490432.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRKTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1266..2288
                     /locus_tag="KPK72_003242"
     CDS             1266..2288
                     /locus_tag="KPK72_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2490433.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2452..3009)
                     /locus_tag="KPK72_003243"
     CDS             complement(2452..3009)
                     /locus_tag="KPK72_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2490434.1"
                     /translation="MTEKEKMLSGKGYYANYELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(3015..4091)
                     /locus_tag="KPK72_003244"
     CDS             complement(3015..4091)
                     /locus_tag="KPK72_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG2490435.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(4092..4775)
                     /gene="modB"
                     /locus_tag="KPK72_003245"
     CDS             complement(4092..4775)
                     /gene="modB"
                     /locus_tag="KPK72_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG2490436.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGILVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4924..5736)
                     /gene="modA"
                     /locus_tag="KPK72_003246"
     CDS             complement(4924..5736)
                     /gene="modA"
                     /locus_tag="KPK72_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2490437.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDNGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5929..6858
                     /locus_tag="KPK72_003247"
     CDS             5929..6858
                     /locus_tag="KPK72_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490438.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWDDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(7019..7468)
                     /locus_tag="KPK72_003248"
     CDS             complement(7019..7468)
                     /locus_tag="KPK72_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490439.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7671..8651)
                     /locus_tag="KPK72_003249"
     CDS             complement(7671..8651)
                     /locus_tag="KPK72_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2490440.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3081 a         1431 c         1067 g         3276 t
ORIGIN      
        1 ataaaaatac taggacttat gatttcaaat ggacttatag ctttatctgg tagtcttatg
       61 gctcagttct tgggattctc agatgtaaat atgggtatag gaactttagt tcttggaatt
      121 gcctctatca taattggtat tacattattt aagaaattta cttttatcaa agatactact
      181 gcaataattg ttggctcttt tatataccaa ttcactatat actttgcaat gagtttagga
      241 atgttatcaa ctgacttaaa actaataact gccatagtta taatagcttt cctagctact
      301 ggtaacttaa atatttcact aaaaaaaaac aaatgcaaag ctagtaccaa aaataaatca
      361 gaaaaaagag gtgttgtaga tgttacaaat taaaaatctt tcaaagagtt tccctaatcc
      421 atatggtgaa cctaatacta tctttgaaaa tttatcaatc gatatagaag atggtgaatt
      481 tgtaagtatc ataggtagta atggaactgg taaatctact ttattaaata taatatccgg
      541 gcttataaaa gaatcttctg gtcaagtatt attaaataaa cataacttgt ctaatttagc
      601 tgaacacaga aaaactcaaa tagtaagtag agtttttcaa gacccaagtc ttggaacttg
      661 cccttctatg actgttagag aaaacttgtc tttagctctt aacaaaggaa aactattaaa
      721 tctaagaaaa tgtcttcgtt acaaaagaga tttcttagaa aatttattgg aaggtgtatc
      781 tcttgattta aaaaaatacc ttgatgttca agtaaaattt ttatcaggag gtcaaagaca
      841 atcattatca ctaattatgt cgtgcttaac aaatccatca gttttattac ttgatgaaca
      901 tacagcagca cttgacccaa aaacatcaaa tgaagtaatt gaattgacta ataagattgt
      961 aagggaaaaa aatataacta cacttatggt tactcacaac ttaaaacacg ctcttcatta
     1021 tggtgataga ttgataatgc ttcataaggg tgaagttgta ttagatgtaa aaggtaaaga
     1081 aaaagaacaa ttaacagttg aagaaatctt agaaaaattt gaatatgcag tttaaaataa
     1141 tatatctatt gtattaaact cggctttaaa ataaagttaa aattacaata attatggtta
     1201 ctttgtaata attcatatga atatatgaac atatataata ataaattttt gagggggata
     1261 taaaaatgat taataaaaaa agattggcaa gtttgatttt agctggtgct cttagtatat
     1321 ctatgcttac agggtgttca caaggaggag actctggtaa ttcaaaacaa gaaagtaatt
     1381 ctaaaggtaa agaagttaaa aaaattggta taactcaact ggttgaacat ccagcattag
     1441 atgccactag aacaggtttt gtaaaagcat tagaaaagaa cggatttaaa gatggagaaa
     1501 atattgatat agatttccaa aatgctcaaa atgacatgcc tactacacaa agtattgcga
     1561 gtaaatttgc atctgataag aaggacttaa tatttgctat atctactcca tcagctcagg
     1621 cagcatttaa tgctactaaa gatataccta tacttattac tgctgtgagt gaccctgttg
     1681 ctgctggatt agttaaaact cttgaaaagc caggcacaaa tgtatctgga acatcagatt
     1741 ttgtttctgt agacaaaggt ttagaacttc ttaaaatatt tgctcctaag gcaaaaacta
     1801 taggagttat gtacaatact agtgaagtaa attccaaagt tcaagttgat gctttaaaag
     1861 aatatgcatc taaaaatgga tttaaagttg ttgaaaaagg tataactact tcaaatgaag
     1921 ttaaccaagg tatttctagt ttagttggca aaatagacgt attatatgtt cctacagata
     1981 acttagtggc ttcttctatg cctatagtct ctaaaatagc tactgagaat aaaatacctg
     2041 ttatagcagc tgaatctggg ccagttgaaa aaggtgctct tgcttgtcaa ggtatcaact
     2101 acgaaaaact aggttacaaa acaggtgaaa tggcagttaa aattttaaat ggcgaatcag
     2161 tatctgatat gcctgtggct acatcagatg atacagatat cattgttaat gaagatatat
     2221 taaaagcttt aggtatggaa aaaccttcta atgaaaatat ttcttatgta aagactaaac
     2281 aagaatagaa agatattttc tatatacgat taatatgcct gtttctataa attaatttat
     2341 agaaacaggc atatattttt attttaatgt attatctatt taaagattat atgtaaatgc
     2401 tcttaatatg attgtataaa ttttacttgt ataaacttta tacaatcata tttattcttc
     2461 tattttcttt attactctac atggatttcc aacagctaca gtatttggtg gtatatcttt
     2521 tgtgactaca cttcctgcac ctattactac attatcacct atagtaattc ctggtgttat
     2581 gattactccc ccacctatcc atacattatc acctattttt acaggagaac catactctat
     2641 acctgaattt ctaagttgtg catcaattgg atgataggct gtatagattt gaacgtttgg
     2701 agctaacatt acattgtcac ctatttcaat tttacataca tctaaaaaaa tacaatcata
     2761 atttgcaaag aaattttcgc ctacatgtat gttatatcca taatcacacc taatgttttg
     2821 ttctacattt atttgcttgc caactgaacc aaatagttgt cttaaaatat cttctctttt
     2881 ttcatattca tcctctaaag tattattaaa caatctagtt aattttttac aatactctct
     2941 ttcttttact aacaattcat aatttgcata gtatcctttc ccagacaaca ttttttcttt
     3001 ttcagtcata tagtttactc ctttttgtta taattgtatc taaagcaaaa taaatattcc
     3061 tgtggaaaat ctaaatatat tctgtcacct ttcttaaatc tcattttact tttttccaat
     3121 tctatttgaa taggtacaca ttcgttagat aaatcagttt ttctaacata tatagtatat
     3181 gtaaaaggat tttctacaat gttaatcaat tcgaaacata acttttcatt taaatcttca
     3241 ttagaatcca agaccttaat atgatgctcc ctgattccca catattctat attatcacct
     3301 atttctctat ttaatgtcaa ctcgcatccc cagtctgtag cataaatagt atttttatcc
     3361 agtctattta atttggatat atttttacac cctgttatct tagcttctat aaggtacttt
     3421 ggagattcaa agagctcttt tttaggtctc tttggaagac cttctccttt gttgtacact
     3481 ataatatcat cacaaactcg gtaagcttct tcaatatcat gagtaacaaa tagtatatct
     3541 cccttgtaat cttttaacat attcataagt tctttttcca tattgcttct taaatgatgg
     3601 tccaatgctg agaaaggttc atctaaaagt aatatatctg gagatgttgc caatgctcta
     3661 gctagtgcta ccctttgttg ttgaccacca gataattgcc aagggtatct cccctcaaac
     3721 ccatttaatt taagtatatc taagtacctt gctactattt cctttttttc actcttactt
     3781 agttttaaaa gcccaagttc tatattttga cttacagtca tatgtggaaa caaagcatag
     3841 ttttgaaata aaaatcctac ctttctatct tgtgttgata aatttatctt cttttcagaa
     3901 tcaaatagaa ctctatcatt taatacaatt ttacctcttg ttggtttctc aagccctgct
     3961 atacatttta atgtcatact ttttccagaa ccagattctc ccaaaaaccc cagagtacct
     4021 ttttcttgtt taatttctac ctttagttta aaagaggata aatctttttc aatatccacg
     4081 tataagctca actatccaca cctctttcct ataattttct gctgaaattc cgaccaataa
     4141 tttagtaata aaatcattgc tatagatatt gctacaataa tcataaccca catcatagct
     4201 ttattcatat ctccgccttc aacagcaaaa aatatagcta ttggcatagt ttgagttttt
     4261 ccaggtatat ttccagctat cataagtgta gcaccaaatt ctccaagagc tctggcaaac
     4321 gacagcacca atccaccaat tattccaggc catgctaatg gaactgcaat cttaaaaaat
     4381 actttccatt cattaagacc taatgttcta gcagctgaca acatattatt atcaatctgt
     4441 tcaaaagctg accttgaagt tctatacatc ataggaaaag acaccactac tgctgaaatt
     4501 acagttgctg tccaactaaa tattaaagtc ctatcgaaat ttaataaaaa ctttcctata
     4561 aatccattct tcccacatag taataataaa aagaaaccta caactgttgg tggcaaaatt
     4621 agtggaagtg taaatagtcc atctataaga cccttccact tacctctata gttagccata
     4681 atatatgaca caagtattcc tattacaaaa gttattattg ttgacaaaat agatgtctta
     4741 agtgatatcc ataagggtga ccaatctgtt cccatctttc cctccttaat tttaaatgga
     4801 aatagtgaaa ctaataactc tattaatttc actatttcca ttttattata atacacaatt
     4861 attttcaatt ttcatttatt tatatataat ttatacttaa ttacacataa tttatttcta
     4921 agtttattct acttttttat atccaaattc ttcaaaaatc ttttgtcctg actcacttaa
     4981 taaaaattct tcaaatttct tagcagcatc tacgttttta ctagctttaa ttactgaaac
     5041 tggatatgta ataggcgaat gagttttttc gtctgttttt tcaactactt ttatcttatc
     5101 attattgaca gtatcactga agtaaacgaa tccaacatca gcatttcctg attgcaccca
     5161 tgctaaaact tcttttacat cttttgcaaa tactagctta tcttttaact tatcttttaa
     5221 atttaaatta gttaacacct catctgcata cttaccagct ggaacacttt cagcttcacc
     5281 aacagcaatt ttcttcactt tatctgtagt caaatctttc atacctgata cactactgtc
     5341 agcagaagaa ataagaacta aatcattttt tactaaatct ttcatagtat cagaaactaa
     5401 taacttctct tcatccaaag ctttcatttg tttttgacct gctgaaataa ataaatcaca
     5461 aggtgctcct tgttctattt gttgttgtaa tgagcctgat gctccatagt taactgtaag
     5521 ttttacattt gaatcaactt ttttatattc ttcttcaatc tttgccattg cttcttttaa
     5581 acttgcagca gccgatatat ttaactctac tgaatcacta cttttagttg ttttttcttg
     5641 ttttccatta tcttttttgt cttcattact attgcaccca actgtcccca aagttaaaca
     5701 tgcaactaat cctaatattc ccaatatttt tttcataaca atctcccttt ttgtaaattt
     5761 gatacacatt ttaacatgtt ttattatgtt ttatcatgtt ttgttatata tagtttaatt
     5821 atatatacat gataataaat tttcaagtaa tttatttaca ctacttttaa atattttgct
     5881 acattttcct ttttttaatg ttaaaataac cttatatgga ggtggattat ggactcatta
     5941 tcatctttga cacctgtcga ggtagctgaa ttattaaaga taacaaaaaa tactgtatat
     6001 gaattaatca aaagaggtga acttccttct tataaagtag ggaaaaaaat aagagttgat
     6061 actaaagatg ttgaagaata tatcaacagc caaaaaactg gtaaagtaaa ggatacacat
     6121 ttcaacaata aaaatataga tataaataat aatttcaaaa ttattatttc tggtcaagat
     6181 gtaatattag acattttagc taggtctgtt gaaaaaaaat tggatggaat aagtacattt
     6241 cgctcttata taggtagtta taatgctttg tatgagttat ataatggcag agtttctata
     6301 gcatcatccc atctatggga tttcgaaacg gatgaatata atagcacttt tgttaaaaag
     6361 ctattgccag gaataccatg tgtacttata aatctagctt atagaatgca aggtttctat
     6421 gttgctaaag gaaatcctaa agagataaca acctgggatg atttaacaaa accaagtata
     6481 accattgtaa atagggaaaa aggttctgga actagggttt tactagatgg taaacttaga
     6541 cttctaaact ttaatggtaa acatataaaa ggttatgata atgaagaatt atcacatctt
     6601 ggagttgcaa gtactgtttc tcgtggaata gcagatgttg gtttgggaaa tgaaaaagct
     6661 gctttacaag taaataatat cgactttatt cctcttcaca aagagcgcta tgatttagtt
     6721 attaaaaaag aagacttaca aaatccagtt taccaaacta taattaacat aataaactct
     6781 cctgagttta aagctgaact tcaaggtctt ggcggatatg atttaactga tactggaaaa
     6841 actatagcaa aagtttaaga ttaaaatcgc aatctatttt ataagattta taatttttca
     6901 caaaatttat aacttaaaca cgaaataaat tgcgatttta gtgtctcaaa aaatttatgt
     6961 ctttcatttt atcatccaat acaaaatata atataattaa ttgtgcaatc atattatatt
     7021 aatatatatt tacataatag tatttttctt taatgtagat taacatatga tatgttttaa
     7081 acatcaatcc ccttgctttc ttatatatat gtggcaacac atatgagttt aataaattat
     7141 atacaatgtt gctttcttct tgtatttttt caaccaacaa gttatttata aaattacctt
     7201 tctcattttc aataatctca ctccaattta tcttaacttt taatatatat cttcccacta
     7261 ggtatacaca ctctacaata aataataaaa atacaatgca gtcagtacct gttgtcttat
     7321 ataaattacc cgatactaat ggttttgcga agaaattata aaaattcaaa gtatcacccg
     7381 tataatcaaa aagtattaat agcactccta gaactctcaa tactttaata ttcaacatgt
     7441 ttttatcttt attatttttg tttttcatat atactacctc tcttttcttt agatttgatt
     7501 gtaatctaat actaactctt ttatttgagg tataaatgaa tttaattggt agttattttc
     7561 atttttacta gaaattaatt cacattacta aacaaaactt atatttaaac ataaaaatag
     7621 cctatctatt ttttaataaa tacaaaaata aataagctac taatattcaa ttaactttta
     7681 tcacttaaaa cattactatc tataaattta aatattttat tgaagtatac tgtactatca
     7741 tgttcaaatc catcaacatg accaatacct tctataagcc ataggtcttt tttatcatgt
     7801 ttaactaaat catacatttt cttgctattt tgataattac aaacagtatc ttttgttcca
     7861 tgtataaaca gtatgggtac attagatttc tcaactgact catatggttt tacatcactg
     7921 taccaaaatc cagatttaaa cttattatat aaatctccac aagtcaccac ataattgatt
     7981 aatatatctg gtattcgttt ttctagtact cccattctaa ttgcatcttt catttcagaa
     8041 taaggagaat ccagtatata aaaatcgacc ttatcatctt ttgaatttat ctctgaatgc
     8101 atagcagctg tccctgcccc catggaaaat ccatgcacac ctaatctccc ttcaggaaat
     8161 ttatttttta caaatttaac tagactatct aaatcatacc tttcataaag accaaatgtg
     8221 taattatctc caccactatt accagtattt ctttgattat aaactaatac attataacct
     8281 ttatctaaat atctatatgc tacctttaac atttcatagt aactacttcc aattccatga
     8341 acaagtatca tagtatctct tgtctttata tttgaagtta taaaaagact ttcaagcttg
     8401 taattattag aactttgtat cattaattca gatgtcttgt agttttttaa tttctccagc
     8461 ggtttatctt ttctctcaga aaatattttt actatatctt cgttttttat taactgctct
     8521 aaaccaactg aactattata cactaatttg ccaaatatat aagatacaat taataggatt
     8581 aatagaaaca caattgatat aattataaat ttatttctta aactaataca atttttaaaa
     8641 ctatttttca tatttattta ccatacaatt ttcctatatt atattttcta agtaatagca
     8701 taattgatta tactattctt ataatttcta ttatacctac ttatttatta aaaatacaaa
     8761 ttatatttta ttaattttta ctacttgtaa attattatct tatacaatga atttgtatta
     8821 tataataagt atttttagga ttctgttaca taata
//
LOCUS       DAEMHZ010000149         8797 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000149 DAEMHZ010000000
VERSION     DAEMHZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.150"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..311
                     /locus_tag="KPK72_003250"
     CDS             <1..311
                     /locus_tag="KPK72_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2490441.1"
                     /translation="DKNDDFSSNQSIRVLDQIANDIATLFNTKYLGEVPNDKSGRISF
                     WNDVVKHHEQLQNMRAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS
                     "
     gene            333..803
                     /locus_tag="KPK72_003251"
     CDS             333..803
                     /locus_tag="KPK72_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2490442.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            857..1114
                     /locus_tag="KPK72_003252"
     CDS             857..1114
                     /locus_tag="KPK72_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490443.1"
                     /translation="MVKIKDKYEIKDSIYFDYSKKRPIEECVGEMYRKVGLFLVDIAD
                     KLALDTIEGSSLKPITIKIQIDEDGIATIEKETKYLVMEVE"
     gene            1119..1559
                     /locus_tag="KPK72_003253"
     CDS             1119..1559
                     /locus_tag="KPK72_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2490444.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKPIEWELKAIDSD
                     RDRQLRKDSAIRVPVLNKKGKSTGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            1880..2041
                     /locus_tag="KPK72_003254"
     CDS             1880..2041
                     /locus_tag="KPK72_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490445.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFTKVKSHSTRGKSKSGWELD
                     FKIKFHKFK"
     gene            2188..2421
                     /locus_tag="KPK72_003255"
     CDS             2188..2421
                     /locus_tag="KPK72_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490446.1"
                     /translation="MDKDIELTGKDLYCIARHVQVYILKKEDEILKKEDNPCCRCEYR
                     FECYEKKICVYHWTTFKKLSKITGLKMGPGIKI"
     gene            complement(2418..2627)
                     /locus_tag="KPK72_003256"
     CDS             complement(2418..2627)
                     /locus_tag="KPK72_003256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490447.1"
                     /translation="MVFESIYEYCSVADENIMATAEFSEIPGIGITPAERDLKFIRLD
                     SPCSDECPFWGTFDCELVKVALKMY"
     gene            2895..3464
                     /locus_tag="KPK72_003257"
     CDS             2895..3464
                     /locus_tag="KPK72_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBG2490448.1"
                     /translation="MNDTLEYIFCSECGAKCLKESKFCSQCGTKIFDIKNAGESMTEE
                     KEYDLKGVDLQHVMQETSFIKASSIRRLKELTGIDLEDCRKILEEPYRKYYNENSDIL
                     EEKRKKEDDMSKIRRAKEEKIEKEKVVCCPKCGSTSLTAQKKGFGIGKAVVGASLTGG
                     IGLVAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            3542..5911
                     /locus_tag="KPK72_003258"
     CDS             3542..5911
                     /locus_tag="KPK72_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG2490449.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAK
                     IVSRIGMNAGKAFSSTREIVSFAEQLNKKFVIAGASTEEMNSALLQLTQGLGSGVLRG
                     EELNAVFDSAPNIIQSIADYLNVDIGKIRSMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTIGQIFTSIKNNAVMIFGAIQKKIEDTVSSRGFRTFIVNITDSLYVLGSVGYKV
                     FNGIIDLLSSPTFQGFFNLMIVGVSLVAQGLGWLLTTIGSVINFIAQGWSIIQPLLIT
                     SIVLWGLYKAAILAGAIVTAIQVGWTALQAFWTNLLNGSLLTNIMMNIAAKISTDALS
                     GSMLLLITTIVMVVATIVLVIAAIFVGVAIFNHFAGTSISATGVVVGCFYFLGACIYD
                     VFAAVWNIVMAFAEFFVNSFNIVIYNVQLLFYKFQNFVIDSMGDVGGSFDNCATALAN
                     AFVSAANIAIKGINGVIKALNLIPGVNIKTIGSLDKIDSFVKQYKDYQKTLKEPVKPA
                     EWKAPYAEIKDPFNSYKKGYEVGRNLENKIKDAFDINKIAEKAKKDLELDDLWDKKYG
                     LGEGIGSGEGIGSGGLNSPLNDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQE
                     TINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            5923..6612
                     /locus_tag="KPK72_003259"
     CDS             5923..6612
                     /locus_tag="KPK72_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2490450.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNRKVSLEEYTIVEDAENGSDFKVAIKLKQYRDYSTKKLVPAPPKSDTGRPNVKIEPK
                     RVDSVNAPSGKTYTVKSGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKKVPKY
                     TIYKGQVLKLV"
     gene            6605..8566
                     /locus_tag="KPK72_003260"
     CDS             6605..8566
                     /locus_tag="KPK72_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2490451.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVIDGITWEIERRGTPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVFKNYTASEIIKMLADDFKLKCKSIEDTKYKISRIEENKTLFDMVLTALDDTLRE
                     KKEMYILYDDFGRLVLKNVASMKLDVVMNNDVIEDFDYNSSIDSDTYTQVKLLKEDEN
                     TGQRISTTVKDSVHIRSWGVLQLFDTVDSNVTKAEMDKKAEMLLKLYNKKTKSLSLKN
                     AIGDIRVRAGCLIPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNSNNKDEKKNGTAQSTTNKEDSDMINKLNKVFKGKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFSSVDEGIKRGISNLSRNYINTG
                     RKTLESIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSAYSSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNNNANTSTNNNSKADKLISIAKSKLGCKYVWGAEGPNTFDCSGFTQWC
                     YKQVGIKIPRTVATQSKAGKAVDLKDRSKWKAGDLLCRVGGGNSNHVMMYIGNGKMIH
                     SPQTGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            8580..>8797
                     /locus_tag="KPK72_003261"
     CDS             8580..>8797
                     /locus_tag="KPK72_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2490452.1"
                     /translation="MSQDLLQVIKKAAMDAVETSDPMMIAFGTIESLNPLVVYIEQKA
                     SFDEFFLIQTETFKRYTDKKIGDKLVLIR"
BASE COUNT         3475 a          990 c         1740 g         2592 t
ORIGIN      
        1 atgataaaaa tgatgatttt tcaagcaatc aaagtattag agtacttgac cagattgcta
       61 atgatatagc aactttattt aatacaaagt acttgggtga agtaccaaat gataaatctg
      121 gtcgtatctc gttttggaat gatgtagtta agcatcatga acaactgcaa aatatgagag
      181 caatagaaga tttcaaagct gatgatgttt ctgtagaacc tggaagcgac aagaagactg
      241 ttgtagtaag tgatgctgta aaagttatta gtgctatgag taagctttat atgactgttt
      301 cagttagtta acaataagaa aggagaataa taatggcaca acaaataaaa gcaagagata
      361 caataagtgc atctaaggca gagtgttttg taactataaa aggtaaaaga tataatttta
      421 tgcaagctat taacttagaa gctaaaatgg aaaagaataa gagtgagata cctatattag
      481 gtagtactac aaaaggaaat aaatcaacag gaagtaaata ttcaggaaat gcaacatttt
      541 attataatac ctctatattt agagaattgt tgtatgagta taaagaaact ggtgaggata
      601 tttacttcga tatacaaatt accaatgaag acccaacaag ttcagtgggt cgtcaaacta
      661 taatactgga agattgcaat atggactcag gcataattgc taaatttgat gctgatgggg
      721 agtatttaga tgaagatatg gactttactt ttgagaattg gaaattagta gaaaagttca
      781 atatagcaaa tggtatggaa taagacgcac atttataaat tatagatgtg tgttttttat
      841 ttatacaaat aaggagatgg ttaaaattaa agataaatat gaaataaaag attcaattta
      901 ttttgattat agtaagaaaa gacctataga agaatgtgta ggggaaatgt atagaaaagt
      961 agggttattt ttagtagata ttgctgataa actggctctt gatacaattg aaggttcgtc
     1021 attaaaacca attacaataa aaattcaaat agatgaagat ggtattgcaa caatagaaaa
     1081 agaaacaaaa tatttggtga tggaggtaga ataagaatat gggagattta aacgcttttt
     1141 taagtcaaaa tgcaataaaa gtagagaata gaaagtatgt ggcaagtgaa agatttatag
     1201 gtgaagatgg gaaaccaata gaatgggaac ttaaagcaat agattcagat agagatagac
     1261 aattaagaaa agattcagct ataagagtac ctgttctaaa taaaaaagga aaatcaacag
     1321 ggcaatacac tagtgaaaca gattttaata cctatacttt gaaactgtgt gtagaaacta
     1381 tagtatttcc agatttgcat gatgcagaac ttcaaaatag ttatggtgta atgggggcag
     1441 aggagctatt aacaacaatg ttaactcctg gtgaatatac agacctttca agtgaagtag
     1501 gagaggtgaa tggttttgat aggacctttg aggataaagt agaagaagca aaaaactaat
     1561 tgaaggaggc gattatgatg ctagtgtagc tcattattgc cttcacaaat tcaaatggaa
     1621 accacatgaa tatacagatt taccagactt cgagagggca tttgttgctg cttctataga
     1681 tattaaagta gaagaagaaa taaaagaaga aaaaaagact gctaaagaag ctagaaggag
     1741 tagaagaaga taaaatatag gtaaaatatg taagaattat atgttataat aattgtagca
     1801 agaagatgta atctacaatt tatagagtgg agttcatact gggataaaac ctacttccta
     1861 atgaaaggag gtgggaagta tgaataactt tttacttaat gtaatagctg gcgttattgc
     1921 tagtttaata ttttgcttaa tttgtaaagt atttacaaaa gtaaaaagcc actcaactcg
     1981 cggcaagagt aaaagtggct gggaacttga ttttaaaata aagttccaca aatttaaata
     2041 attactaatt gtttaaatta tgaacttcac tctacgtcca aatagattgt agttcttctt
     2101 gctattatta taccacaaat tagaaaaaat atgcaaagta cttgttttaa tagcaagtat
     2161 ttttttgtac aaaaagaagg tgattaaatg gataaagata tagaacttac aggaaaagat
     2221 ttatattgta ttgctaggca tgttcaagtc tatatattaa aaaaagaaga tgaaatattg
     2281 aagaaggagg ataatccatg ttgtagatgt gaatatagat ttgagtgtta tgaaaaaaag
     2341 atttgtgtat atcactggac cacatttaag aagttatcta aaattacagg tcttaaaatg
     2401 ggtccaggaa taaagattta gtacattttt agagccacct taactaattc acaatcaaaa
     2461 gttccccaaa atggacattc atcagaacag ggagaatcta agcgtatgaa ttttaaatct
     2521 ctttcagcag gagtgatacc tatgccagga atttcagaaa attcagcagt tgccattata
     2581 ttttcatctg ctactgaaca gtactcataa atactttcaa ataccatata ttcaccatcc
     2641 tttcataatt agtttagaag tatctaacaa tacctcttga attaattata gcatggtata
     2701 ttagggagat ttgtttatat taagaataat atgtaaaaat tttgtgtttg tagtatgtaa
     2761 actaaataaa catagaaagc acttactttt atagtaggtg tttttttatt gaaaggatgt
     2821 gattataatg taaaaatttt acttatatag tataataatc ttataaaatt attgtactag
     2881 ggggacattg tactatgaat gatacattag aatatatatt ttgtagtgaa tgtggggcga
     2941 aatgtctgaa agaaagtaaa ttttgcagtc aatgtggaac aaaaatattt gatataaaaa
     3001 atgcaggcga gagtatgact gaagaaaaag aatatgattt aaaaggtgtt gatttacaac
     3061 atgtgatgca agaaacaagt tttataaaag catcttctat tagaaggttg aaggaattaa
     3121 ctggaataga tttagaagac tgtagaaaaa tacttgaaga gccatatcga aagtattata
     3181 atgaaaattc tgacatactt gaagaaaaaa gaaaaaaaga agatgatatg tctaaaatta
     3241 gaagagctaa agaggaaaaa atagaaaagg aaaaagtagt ttgttgccct aagtgtggtt
     3301 caacatcctt gacagctcaa aaaaaaggtt ttggtatagg gaaagcagta gtaggagcta
     3361 gtctaactgg tggtataggt ttagtagctg gaaatttagg agcaaagaaa gttagagtta
     3421 catgcttgaa ttgtgggaaa cagttttggg caggcaaaaa atagatgtat taattataaa
     3481 aacacttact aatttagtaa gtgtttttat tatctaaatt aacagaaagg agagtgaaaa
     3541 aatggctaca atacagacat caattcgcat atttgatgga atgacacctg catttcggca
     3601 tatgacaact gctatgaata tagtactgag ttcatttgag caattacaaa gaacttctag
     3661 taatgcagta aatgctaata gcataataag agctagagaa gaactagcaa gagcagaagc
     3721 tggatttgat agattagaac gacaaataag agaatcagat aatcaacagc gaaaacttaa
     3781 tgaggatata aataagggtg caagttctac agatagatta gttggaagtg caaagaagct
     3841 agcagcaact tatttaggta taagaacatt aggaggtcta ggaaatttaa gcgaccagat
     3901 gacaagtact aatgcgagac ttggcatgat aaatgatggg caacaatcag atgctggact
     3961 taataaaatg atatttcaat cagctgaaag gtcaagagca tcttatttgg atactgcaaa
     4021 gatagtttca cgaataggta tgaacgcagg taaagcattc tctagtacta gagaaatagt
     4081 aagctttgct gaacaactta acaaaaagtt cgtaatagca ggtgcaagta ctgaggaaat
     4141 gaattcggca cttttacaat tgactcaagg tttgggttct ggtgtgttga gaggtgaaga
     4201 attaaacgct gtatttgatt cagcacctaa tataatacaa agtatcgctg attacctcaa
     4261 cgtggatata ggaaaaataa ggagcatggc aagtgaaggt atgttaacag ccgacattgt
     4321 aaaaaattct ttgcttgcag cagcagaaca gactaacgca gaatttgaga agatgccgta
     4381 tactataggt caaatcttta catcaattaa gaataacgca gtaatgattt ttggagcgat
     4441 acaaaaaaag attgaggaca cggtctcaag taggggattc cgaaccttca tagttaatat
     4501 aacagattcg ctatatgtat taggttcagt tggttataaa gtgttcaatg gaataatcga
     4561 tttattgagc agcccaacgt ttcaaggatt ttttaatctg atgattgttg gagttagtct
     4621 agtagctcaa gggttaggat ggctattaac aactatagga agtgtcatta actttattgc
     4681 acagggatgg agcataatac agcctttatt aatcactagt attgtattgt gggggttata
     4741 caaagctgct attttagcag gtgcgatagt cacagctatt caagttggtt ggacagctct
     4801 gcaagcattt tggactaatt tattaaatgg ttcattatta acaaatataa tgatgaatat
     4861 agcagcaaaa atatctacag atgctttaag tggttctatg ttacttctaa ttactactat
     4921 agtaatggtt gtagctacaa tagtcttagt aatagcagcg atatttgttg gggtagcaat
     4981 atttaatcat tttgcaggaa cgagtataag tgcaactgga gtagtagtag ggtgttttta
     5041 tttcttggga gcttgtatct atgatgtatt cgcagcagtt tggaacatag ttatggcatt
     5101 tgcagagttc tttgttaatt catttaatat tgttatctat aatgtacagt tattatttta
     5161 taaattccaa aactttgtaa tagactcaat gggtgatgtt ggaggaagct ttgacaattg
     5221 tgctacagct ttagcaaatg cttttgtaag tgcagcaaac atagcaataa aagggattaa
     5281 tggagttata aaagcactaa acttaattcc aggtgtaaat ataaagacaa taggaagtct
     5341 agacaaaata gattcttttg taaaacaata taaagattac caaaaaacat taaaagagcc
     5401 tgtaaaacct gccgaatgga aagctccata cgcagaaatt aaagacccat ttaactctta
     5461 taagaaaggt tatgaagtgg gtagaaactt agaaaataaa ataaaagatg cttttgacat
     5521 caataaaata gcagaaaaag caaaaaagga tttagaactt gacgatttgt gggataaaaa
     5581 atatggtctt ggtgaaggaa taggttctgg tgaaggaata ggttctggtg gacttaattc
     5641 tccattaaat gacgcagcaa aaggtgcaaa agatactgcg ggaaatactg caaagatggc
     5701 taaaacaatg gataaaagcc aagaagactt aaaatactta agagacatag cagaacagga
     5761 aacaatcaat agatttacag gagtaaacat aaaaattgat atgaacaaca caaacaacat
     5821 aagcaaagac acagatgtag acggaatagt taatgttctg actgaaaaat taaatgatgc
     5881 tatggttgta tcagcagaag gaatagttta ggaggtgttt aaatggctta tgatttttat
     5941 ttagatggag tacaattacc aatcgcacct ggcaaattag aaattaaagt tacaaataaa
     6001 aataagacag ttgatttgat aaatactgga gaagtaaata tattaaaaaa agaagggtta
     6061 tccgaaataa gttttgaagc agaatttaca cacaataaac taccattcta tcgtggcact
     6121 tttagggatg ttcaattctt tttaagtaag ttagagttgc taaaaacaga ttgtaagccg
     6181 tttcaattca ttgtgtctcg tgaactcggg aataaagtac tatttaatac aaatagaaaa
     6241 gtatcattag aggaatacac tattgtagaa gatgcagaga atggttcaga ttttaaggtt
     6301 gcaataaaat taaaacagta tagagattat tcaactaaaa agttagtacc tgctcctcct
     6361 aaaagtgata ctggtagacc taatgtaaaa atagaaccaa aacgtgttga ttcagtcaat
     6421 gccccaagcg gtaaaacata cacagtcaaa agtggtgatt ctctttggtc aatctgtcaa
     6481 aaacaacttg gtaatggttc attatataag aaagtatatg aactaaataa atcaatgatg
     6541 gataaagcta acaagggtaa aaaagtacct aagtatacca tctacaaagg gcaggtgtta
     6601 aaacttgtct gatgaattag tattagcaaa tgatagggat gtaagattag ttattgcaca
     6661 ttgggaagac ttttacgaac cagttgttat tgatggtatt acatgggaaa tagaaagaag
     6721 aggtacacca agtaaattag agtttactat agtaatggat gatatactac agttttgtga
     6781 aggtaattca gtgagattat attataaggg aataggtata ttctatggat atatatttca
     6841 aaagaaaaga gataaagaaa atcatattaa aattgttgct tatgaccagt tgagatattt
     6901 taagaataaa gatacttatg tttttaaaaa ttatacagca agtgaaataa taaaaatgtt
     6961 agctgacgat tttaaactaa agtgtaaatc tatagaagat actaagtata aaatatctag
     7021 gatagaagaa aataaaacac tttttgacat ggtactaaca gctttagatg atactctgag
     7081 agagaaaaaa gaaatgtata tactctatga tgattttggt cggctagtat taaagaatgt
     7141 agcttctatg aaattagatg tagttatgaa taatgatgta attgaggatt ttgactataa
     7201 ttcttcgatt gatagtgata cttatactca agtaaaatta ttaaaagagg atgaaaacac
     7261 aggtcaaagg atttctacta cagtaaaaga ttcagttcat ataagaagtt ggggagttct
     7321 acaactattt gatacagtag atagtaacgt gacaaaagca gaaatggaca aaaaagcaga
     7381 aatgctctta aaattataca ataagaaaac taagtcatta agtttaaaaa acgcaattgg
     7441 agatattaga gtgagagcag gttgtttaat acctgttttt ttaaatctag gagatattga
     7501 gttacaaaat tatatgctag ttgagaaagt aaagcacaca tttgaaaata acagtcattt
     7561 tatggattta acacttgttg atggagacga atttgcttct tattcttcat cctcatatag
     7621 tagtggaaat agtaacaata aagatgaaaa gaaaaatggt acagcacaaa gtactacaaa
     7681 taaagaagac agtgatatga taaataaatt aaataaagtg tttaaaggaa aattatcaaa
     7741 tacaggaagt atatttgtta aatattctaa tgcttacaag gtcaatgcag ctttaatggc
     7801 tgctatttct atacatgaaa caggaaatgg tagttcttca ctttgcaaaa ataaaaataa
     7861 tttctttgga atgaaaggga tgtcttttag ttctgtagat gaaggaataa aaagaggtat
     7921 aagtaattta tcaagaaatt atattaatac aggaagaaaa acattagaaa gtattagaga
     7981 caaatatgct ccgctttatg atagtcctct taacaaagat tgggtaccag gagtaggaaa
     8041 gttctataaa caaataacag gaagtgcata tagttctaat agtgcaggta caggagttgg
     8101 aagtaatgaa gaagcagaaa agaatttaaa agatttaact tatcaagttc aaaacaataa
     8161 taacaatgct aatacatcca caaataataa ttctaaagca gataaattaa ttagtatagc
     8221 taaaagtaaa ttaggttgta aatatgtttg gggagcagaa ggaccaaata cttttgattg
     8281 ttctggattt acgcagtggt gctataaaca agtaggtata aaaattcctc gaactgttgc
     8341 aacgcagagt aaagcaggta aggctgtaga tttaaaagat agaagtaaat ggaaagcagg
     8401 agacttatta tgtagagttg gtggaggaaa tagtaatcat gtcatgatgt atattgggaa
     8461 tggtaaaatg attcattcgc cacagactgg tgatgtagta aaaatacaat cggttgattc
     8521 atatcgaaaa ggaaaagcgt acacacatgt aagaagattt atataagtga ggtgataaag
     8581 tgtcacaaga cttattacaa gtaattaaaa aggctgcaat ggatgcagta gaaacaagcg
     8641 atccaatgat gattgcattt ggaactatag aaagtcttaa tccattagta gtttatatag
     8701 aacaaaaagc atcttttgat gaattctttt taatacagac agaaacattt aaaagatata
     8761 cagataaaaa aataggagat aaattggttt taattcg
//
LOCUS       DAEMHZ010000150         8789 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000150 DAEMHZ010000000
VERSION     DAEMHZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.151"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..954
                     /locus_tag="KPK72_003262"
     CDS             187..954
                     /locus_tag="KPK72_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2490453.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1237..1434
                     /locus_tag="KPK72_003263"
     CDS             1237..1434
                     /locus_tag="KPK72_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2490454.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1463..2539
                     /gene="vorB"
                     /locus_tag="KPK72_003264"
     CDS             1463..2539
                     /gene="vorB"
                     /locus_tag="KPK72_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2490455.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYL
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2542..3261
                     /locus_tag="KPK72_003265"
     CDS             2542..3261
                     /locus_tag="KPK72_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2490456.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3274..3813
                     /locus_tag="KPK72_003266"
     CDS             3274..3813
                     /locus_tag="KPK72_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2490457.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3970..5028
                     /gene="buk"
                     /locus_tag="KPK72_003267"
     CDS             3970..5028
                     /gene="buk"
                     /locus_tag="KPK72_003267"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2490458.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            5046..5939
                     /locus_tag="KPK72_003268"
     CDS             5046..5939
                     /locus_tag="KPK72_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2490459.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5969..7177
                     /locus_tag="KPK72_003269"
     CDS             5969..7177
                     /locus_tag="KPK72_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2490460.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7201..8460
                     /locus_tag="KPK72_003270"
     CDS             7201..8460
                     /locus_tag="KPK72_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2490461.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3417 a          973 c         1741 g         2658 t
ORIGIN      
        1 taattctatt aaaagtaaga ataataagga catagtgagt attttgtttt tattttacaa
       61 attaagtata atatttttgt tttataaaat ataattaaga taaatagata attttgtaat
      121 tttaatatat aatatttagt agagtgtggg agtagcactt aatatttgca ggaggagttt
      181 ttgtatatgg gagaaataat aaatgcatta gatagagccc tagacataat tttgctgtta
      241 tatcatgaaa agagagaaat gggaataact gaaataagca aggctatggg agtatataaa
      301 agtactgtac atagaacttt ggttactctt gaaaacaaag gttttgttat acaaaatgca
      361 gagaatggta agtattggct aggaataaat ttatatgcaa taggtatggt tgtgggagaa
      421 aaaatgtcat tgactgaaat agtaaaacca tatacgaaaa aattaaatca agaatttaac
      481 gaagtagtta atgtatctat attagaagaa agagctcaag attctccaag aagtatcata
      541 atacataaag agtatggttc aaatcaatta ctttcagtaa atccatccgt tggttcaagt
      601 agtgaatgtt attgttcagc agttggtaaa tgtcttatgg catttaatga ttctatagat
      661 tttgaaaaat atagaaaaac accaatacac aagtatacag aacatacaat tgataattgg
      721 gatgatatga tgcttttttt agcaaaaata aaagaacaag gatatgctat agatgatgaa
      781 gaactagaac atggactaac ttgtattgga gcacctatct tagataaaaa taataaagct
      841 attgcagcca taagtttgtc aggtccaact ataagaatga gagaagggga ctttgagtat
      901 aagattaaaa gagttataga aactgcaaaa agtatatctg aactttttag atgattttta
      961 atctcgataa taaaaatagg ctatatttaa attgttattt gtaaatatca atttaaatgt
     1021 agcctatttt tctttaatct aaaattaaat attcttaaaa tttaaaatgt tattgaaata
     1081 tataagcgaa tgtgttatct tttaattaag caataaaaat agaacaaagt tctatactat
     1141 agaacaaaat tgatgcatat atagtattta tacaaaatat aaatattata tatttaaaat
     1201 taagtaaaaa tattacataa aggggagatt tgagaaatgg aaaaagtcaa gttgagtgtt
     1261 gaaagatgta agggatgcta tctttgtatt gaagcttgta aaaaagatgc aataagtctt
     1321 tctgactaca caaataaaaa aggctttgta actattaaag tcgacgaaga aaaatgtatt
     1381 caatgtggcg cttgttatac aatgtgccct gatttagtat ttgagatatt ataaattatt
     1441 tttattagag gggagaaaag gtatgcaaag tttattaatt aaaggaaatg aagctttagc
     1501 agaaggtgct atacgtggtg gctgcagatt ttatgcaggt tatccaataa caccacaatc
     1561 agaaatatta gaaatgatgg catggagaca aaaagaagtt ggaggagtat ttatccaagg
     1621 tgaatcagaa atagccagtg taaatatggt tatgggagca agtgcacttg gagcaagagc
     1681 tatgacttct tcatctggtc caggattttc attaaaacaa gaaggtattg catattgggt
     1741 atcagctggt ttgccagcag ttgtagcatg tgtgcaaaga tatggtatag gtgatggatt
     1801 tataggagct ggtcaagata attattggca agcagtaaaa ggcggtggaa atggagatta
     1861 tcatttaatc gtgttagcac ctaatagtgt tcaagaaaat atggatatgg catatgaatc
     1921 ttttgaattg gctgaaaaat atatgcatcc agtacttata ctttctgatg ctacaatagg
     1981 tcaaatggta gaaccttgta tagcaccacc aatgaaagaa tacgatatgg ataaagctcc
     2041 atgggctgca aaaggaacac ctgtaggaga aaaaaataaa gtaataactg atataacata
     2101 ttttgaagac caagatacat ggcaaaaagg gtatatagaa aaaatgagaa aagttcaaga
     2161 agatgaacaa agatgggaag agatagaaat agaagatgct gaagtggtat ttgtggcata
     2221 cggtatttca tcaagaatag cagaaggtgc agttagagat gctagagcaa aaggaatgaa
     2281 attaggtcta ataagaccaa aaacattgtg gccttaccca agaaaagcat ttaagaaatt
     2341 aagtgattca ttgaagggac ttgtaacagt tgaaatgaac atgatggcac agatgaagga
     2401 agatgtaatt atagcatgtg ataatagatt tccagtatat tcagtagcaa cagctcaata
     2461 tttggcagaa gtaaatcaat taatagattt tgctcaacag attatagatg gaaaagctaa
     2521 gcaagaggag gtattttaat tatggctaaa aaagcaccag caataatgat gtgtgaaaat
     2581 atgttctgtg gaggctgtgg acactctata gtcgatagaa ttattggaga agtattaaca
     2641 gaaatggata tatgtcaaga tacaattata tgttcagata taggttgtaa tcatatgttt
     2701 caatttagta tgaatgtaga tgttgtcgtt ccacctcatg gtaaaatggg agcagcaatg
     2761 gcagctatga aaagagttag accagataaa tatatattta caatagctgg agatggtgga
     2821 gcttatgcaa tagggcttgg agaaactatg tcagctgcta ctagaaatga taatgtaata
     2881 tttcttgtta tgaacaataa tatgtttgca atgacaggag ggcaaatggc tccaactact
     2941 atagaaggtc aatatacagc ttctactcca gcgggtagaa attacgcaga tcatggttca
     3001 acatttgatg ttgtaaaagt tatgggaaat ttagatatag catatctagc aagaggaaca
     3061 ataactaacc ctaaagaaat aaatacaaca aaaagataca tcaaaaaagc tctagaaaaa
     3121 caaaaagaaa agaaaggttt ttgcttaata gaagtgcttg taccatgtcc aacaaactgg
     3181 ggattaaagc cagtagatgc tatgaagaga attgaaaatg aacttgttcc aaagtatcca
     3241 cttggagaat ttattgatta gggaggaaat aatatgttga atgaaattat atgtgctggt
     3301 tttggaggac agggagttct tacagcagga aaaattataa tgtatgttgc atataaggca
     3361 gaaaagaaaa ttacatggtt cccatcttat ggaaatgaga tgaggggagg aagtgcaaat
     3421 tgcaatgttg ttgtaagtga tgaaaaaatt gcaaatcctt gtgtaaaaaa tccagatata
     3481 ctattgggaa tgaacagcgt atcagtagat aagtatgaaa gtttaatgaa aaaaaatggt
     3541 tatatgtttg tcaatgaatc actagtaagt aaggatagaa agtacagaga agacattaat
     3601 gttataaaag tacctgttac tgaaatagca caaaatttag gcaatgaaaa agctgctaac
     3661 atttgcatgt taggtgctat ggtaaagagt ttggatttat ttacaaaaga agaatttgaa
     3721 agtggtatgt gtgaatactt tgaaaatcaa ggcaaaggta aattcaattc taaaaatgta
     3781 gaagcatttg aagctggata cgactacgaa tgaatatttt aaatatttaa aataattatt
     3841 gaaatactta tttttatatg ttagtattta attaataaaa tatagaacgt cgttctggac
     3901 aataggacga agtctttagg tcggaataca agaacgacgt tctgcatagt agaactaggg
     3961 ggtgttaaat tgaaagtttt agtaataaat cctggtggta catccacaaa aatttctgtt
     4021 ttccaagatg aaaatgaaat tttgaaaaaa aatataaccc atacaagaga agatttaaaa
     4081 aatttttcta aagtatttga tgaatatgat tatagaaaac aactgatagt agatatttta
     4141 agtagtgaga atcatagtat aaattctttt gacgcagtag ttggtcgtgg tggacttatg
     4201 aaggctataa aaggtggtac ctatactgtg agtgaagaaa tgattgagga tatgagaaat
     4261 gaaataaatg gggaacatgc atcaaattta ggtgcattat tagctaaaac tatagctgat
     4321 gaaataggag tacaatcatt tgtagtagac ccagtttctg tagatgaatt tgatgatgta
     4381 tcgaggatta caggaatatc agacatagaa aaagcaagtt ggttacatgc attaaaccat
     4441 aaagctgttt caagaaaaat tgcacaaaaa ctagggggaa aatacgagga ttataacttt
     4501 attgtagctc atttaggttc aggaatatcg attgtagcac ataaacaagg caaaatgatt
     4561 gatggaagtg gtggtaggtc tgatggtcca ttttctccag aaaggagtgg tggattactt
     4621 gcatatcctc ttatagaatt gtgttactcc ggaaaatata ctcatgatga aatggtcgca
     4681 aaagtaagtt cgattggggg aatgtacgat tatttaggaa caaaggacat gatagaaatt
     4741 gaaaacagaa tcaaggaggg tgatgaaaag gcaaaactta taatggatgc atttatttat
     4801 caagtagcaa aagaaatttc tatgtatgga gcttcacttt cagggaatgt agatagagtt
     4861 atcttgacag gagggatatc tcactcagaa atggtagtca aaggagtagc agaaaaagtt
     4921 tcatatctag ctccaataga gatagtacca ggagagatgg aaatggaggc acttgctcta
     4981 ggggcactta gagtacttaa tggagaagaa atagcaaaaa cttattaata tttaggagaa
     5041 agagtatgtt aaaagatttt aataatttat tagaagaggc taaaaagctt gaacctgtta
     5101 ttgtaagtgt tgctgttgca gaagacaaag aagtattaaa ggctgtgaaa tcagcaacag
     5161 atttaaacat tataaagcca attcttgtag gagataagga taaaatagaa agtatatcaa
     5221 gagaaattgg attaaaaggt catgaaataa taggttgtca aaatcccgac gaatcagtta
     5281 aaaaagctgt agagatagtt aaaaataagg aagctaaagt tcttatgaag ggattagtaa
     5341 atacaagtgt ctatatgaga gctatattaa ataaagaaac gggtcttaga acaggaagat
     5401 tattaagttt gcttgcagta tatgaattac ctcaatatca taaacttctt tattgtactg
     5461 atagtggaat aaatgtatcg ccaaacttaa atcaaaaaaa agatataatg acaaatgcac
     5521 ttcttactat gaaaagtata ggcatggaaa atcctaaaac tgcgatactt actgcaaatg
     5581 aaatggtaga ccctaagata caatctacag tggatgccaa cctacttgta gagatggaaa
     5641 aatcgggaga aataccaaag tgtatagtgg aaggtccaat agcatttgat gtagcatttg
     5701 atgcacatgc agcagaacat aaaggtatta aaagtaatat tgctggtgat gttgatttat
     5761 tagtatttcc aaatatagaa gcagggaata tattagggaa gtcatggctt agatttaata
     5821 aagctaagtg ggcaggtata gttttaggag caacaaatcc gataatttta ggttcacgtt
     5881 ctgacacagc tgaaataaaa ataaattcaa tagcattagc gtgcctagca tcacaataag
     5941 aaattattat taaggaggaa aaaatattat gaaatatgca aaaagaatgg atatggtaaa
     6001 agcttcagct ataagagatt ctcaaaagaa aatagctcag aaggtagcaa gtggtggaac
     6061 tgttatatct tttgctgcag ggcttcctga cccaaattta tttccaatag aagagataag
     6121 tgaaataact caaaaagtac ttaatgaaag gggaaaattt gccctagcat atggacctac
     6181 taaaggtgaa gatgagttgt tagatctatt agtaaaaaga atgaaagaag aagaaaatat
     6241 tgatacaaaa gcagaaaata ttataataac tactgggtca caacaaggga tagcattaag
     6301 tgctatgatt ttattagaaa agggagatgt agtagttaca gaaaatccaa gttacttagg
     6361 tgcaataaat gcatttagac cttatgaatg tgatttttta ggagtagata cagatgaaga
     6421 aggaatggta acagatgatt tagacaaatt attatctgaa aattctaaca taaaggtgat
     6481 atatgtaata ccaacatttc aaaaccctac aggaaaaaca tggtctttac aaagaagaaa
     6541 agattttatg gaagtcgtga ataaatatga tgtcatagta atagaggata atccttatgg
     6601 agaaataaga tttacaccag aacctctacc tacacttaaa tctcttgata ctaaagataa
     6661 agtattgtac cttgggtcat tttcaaaggt attatgtcca ggatttagag ttgcttggat
     6721 gtgtggtaat gtagacatca tagataaggc agaactttta aaacaaggta tagatttaca
     6781 gtcaaatcaa ttttcgcaag tacaagtaat agaattttta aagaaataca acttaaatga
     6841 acatatagaa aaaataagag cagagtataa gaaaagatgt ctgctaatgt tagatgctat
     6901 gaagaaacat ttcccagagg aagctaaata tacagaacca gatggaggaa tgtttatatg
     6961 ggttgaactt cctgaaagta taaatgtaga tgatttatta gataaagcca ttgatgctgg
     7021 agttgcttat gtttctggag agtccttctt tgcaaataat ggtccaaaaa atactatgag
     7081 acttaactat acaacaatgt cagaagaaca aatcgttaaa ggtgttgaga tacttgcaga
     7141 agttataaaa aaagaactca attatgcttg tgaataaata taaaaagtga gggtttaaat
     7201 atgggagaaa aagaaaacgg tataaagttt ggaaagttaa tgtttcaact tgttattata
     7261 gcaatatctt gggaattagt ttatattata ccatttatcc aatatacatt atatgatcca
     7321 atcctaaaag cattagaatg tacaaacact caactaggtt ttctacttac aatttatggg
     7381 ttaggtaata tatttggagc tcctatagga ggctggttag cagatagatt tgattataga
     7441 aagataattt taggttctgt atttcttaat ggagtagtat catttttatt tgcatttaat
     7501 atgaactatc catttgcagt tgctacttgg gtaggttgtg caatcacatc attagttatg
     7561 aactatcctt ctatggtaaa aatacttaga gttataggaa aagataatca aggtaaggtt
     7621 tatggattta atgaagctat ggttggagtt tctggagtag taatgggagc aatattctta
     7681 tatatttata catgctttgc aactcctaca ttgggtatga gatgggtaat gatatcttta
     7741 ggaatattat caatagcaat gtgtccagta ttatggtttg taattaaaga tgtagatatt
     7801 aaagaagaaa gagaagaagc aggtgaaaaa atgtctgctg gtgattttat aacagtacta
     7861 aaatcaccaa atacttggct agtaggaata agtatattct gtgtatattc attcacagtt
     7921 acaatgtctt attttacacc atatataaca tctgtacttg gaggttctgt agcattatct
     7981 ggtgcattag caataataag acagcatggt ctaaaattat ttggagcacc atttggagga
     8041 tattgtgcag acaaattaaa atctcctaca aaagtacttt taccaatata tgtatttggt
     8101 atagcagtaa tagttttatt cctagtatta cctgcatcaa ctccaatgac tatattcata
     8161 gctttaacat ttgtagtagg tatattaggt tacatgggaa aaggtatata ttatgcagta
     8221 caagatgaag taaaagttcc agttaaatat tcagcaacta ctataggtat agctgcagct
     8281 ttaggatttt caccagatgt attccaattt gcattaatag gacactggat agatacttat
     8341 ggcaataaag gttacacata tacatttata ttccaaatag caattctagt tattggaata
     8401 ctatcttgtt tatatatatt aaaggttaaa aagagaagag caagtaaatt agaagcttaa
     8461 atattcaatt tggtaattaa gtatatataa atacaatact tattttgatg aatgtgattg
     8521 tataaataac ataaaaaata tcaatagatt aagttttata ataatgttta gagaataaag
     8581 tataaaaatc aaaatgttat caagttttct aggttttaac ataaagttac ccctgaaggt
     8641 gtaaaattga gtggcaatta ttatctattt tcttacctac ataaacctat ttaatttaaa
     8701 ttctaagaga ttttcaaaat agatggtagt tgtactcaat tttaaacctc ataataaata
     8761 cgaaaattta aaatctaact agatactat
//
LOCUS       DAEMHZ010000151         8712 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000151 DAEMHZ010000000
VERSION     DAEMHZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.152"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            481..1923
                     /locus_tag="KPK72_003271"
     CDS             481..1923
                     /locus_tag="KPK72_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2490462.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1965..2318
                     /locus_tag="KPK72_003272"
     CDS             1965..2318
                     /locus_tag="KPK72_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490463.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2509..3330
                     /locus_tag="KPK72_003273"
     CDS             2509..3330
                     /locus_tag="KPK72_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2490464.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3588..5471
                     /locus_tag="KPK72_003274"
     CDS             3588..5471
                     /locus_tag="KPK72_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG2490465.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKESILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGVGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLVDKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5696..6376
                     /gene="pgmB"
                     /locus_tag="KPK72_003275"
     CDS             5696..6376
                     /gene="pgmB"
                     /locus_tag="KPK72_003275"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2490466.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            6373..8658
                     /locus_tag="KPK72_003276"
     CDS             6373..8658
                     /locus_tag="KPK72_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2490467.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCIPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
BASE COUNT         3527 a          810 c         1560 g         2815 t
ORIGIN      
        1 aattaaaaaa agataatgat agcaataatt attgttacca agaatctact acttcaagtg
       61 ttaccttagg tatttaagtt gatagtggtt tgcacgtaat aaacttgatg taatatatat
      121 tcttaataat atcgataaaa gatttactta aaaataatat agattatgta aacatataat
      181 ctatttattg tatacaataa aatttcattt tgtaaaacag tgaattaaat atgtaatata
      241 gatatcgatt tttattacaa attgttacta aatataggta cattagaaca aaaatctagt
      301 aaattgtaac taaaatgtaa atttttcctt taatctatac aatattatgg aaactttttg
      361 taatttatga tataataaca aatatataat tattaaatta taataaatga ctatagcgta
      421 gatgtaatac tatgttatag tcatttttga attaaacttg atatatcaag ggggaagaat
      481 atgagaacaa ttagaagagt tttagcatta ggtttaacat tagctatatt tctaataaat
      541 gtacctaatg tagatgcatt aacatcagat acaattaaag gtaacaatat atatgagacg
      601 gctggattaa tagcagataa aaagagttat gatacagcaa ttatggtaaa tatggataat
      661 tctatagcag atggtctttc agcaagtggt cttgcaggtg ctgttgatgc tccaattttg
      721 cttgcacaaa aaaataagat accaaatgaa acaaaacaaa gattaaaaaa tgttaaaaag
      781 atatacataa taggtaaaga attatcaata agtaagtcag tagaaactga actaaaaaac
      841 acaggggcac aggtaactag attgggtgga gatgacagaa taaagactag ttatagtgtt
      901 gctaaagagg taaatggtat caaaaaagtt gatgaagtga tattaactaa tgcatataaa
      961 ggagaagcag atacaataag tgctgcacct gtatcagtaa gagatatagc tcctatagta
     1021 cttacagatg gaaagagtgt gcctttttca acaagcaatg taaaaacata tgcagtaggt
     1081 gggagtatat caatgagcac tagtttagtt aataaaacta atgctaaaag acttggtggt
     1141 tcagatagat atgatacaaa taagaaagtt ataaaagagt tttatccaga tgcatcagaa
     1201 ttttatttga gtgatggata tgatttagta aatgcactta caggttctac aattgctaag
     1261 gaaaatccaa ttgtattggt gtcagaaagt agtgataagt ctatattagc aggagcagat
     1321 aagattacta gattaggttc aataagtgac agtgtgtata ataagtgtgt ttctgctgca
     1381 caaaataatg gcgattcgtc tacaaaaggt gagtcaccta tgaaaaatga gacaagcata
     1441 ttaggtcagc caacagctag tttagaagcg tgcttaaaat gggcaaaatc taaaaaagct
     1501 aatgatttat ttatagagtt aataccaata ttatatgata ctgctgttca ggaaggtgtt
     1561 aatcctgttt tggcagtagc tcaatctgca aaagaaactg gtttttgtaa ttttggtgga
     1621 gtattagatg catcatttaa gaatccttgt ggacttaaaa cttctgtagg tggttctgat
     1681 actgataaga atgcccattc aagatttgat acttgggaag aaggaatatt agctcaaatt
     1741 caacatttat gcttatatgc aggacaagat ggttacccac tttcaaatcc agtagaccct
     1801 agacatgaaa aatctttatt tggtaaggct aaaacagttg aaagtttatc taataattgg
     1861 gctggaggtc aatatggaca agatttagta agaatgatgg gagaaattga ggcaacaaaa
     1921 taattgaaga ttatcaaata tccaataaat aaggattgat tattatgaaa aagagtaata
     1981 tttttagtat catatttcct acagtcataa tggtgctaat gtttttaact tttgctacag
     2041 atatatttaa tattccagat attcaatcta aaagtatttt tgttgtggga atggttttga
     2101 tttttccaat ctcttttttg atacaaggga taatttgtgt attaaataag actaattgga
     2161 ttttatctct tattgtgtct ttagtaactt atatagtatt aatgtatata tttttaaatg
     2221 attctgcata tatatatgtt ttactttata cagcttttta tatgattgga catataacag
     2281 ctaaactcta ttacaaaatt aagactttta aaaattaaat tgaagtatat aaaagtaaca
     2341 ttaagagaag tatccttaat gattttctaa aatcagaaat aggtgcttct cttttttgta
     2401 aaaataaaag tatgtaaata aaatttaacc attataattt tcttttaaca aatgtataca
     2461 ataaacttgt tgtataatta agatagataa aataaaggga gggataatat gaatttaata
     2521 gataatcata ttcatactaa tttttcaagt gatggaaagg attccatgga agatacaata
     2581 aaaaaagcta taagtattgg tgttagatat ttgactttta cagaccattt agaacatgat
     2641 gaagatagag gattttgcat aaattataat gactatgttc cagtatttaa taagtttaaa
     2701 gaaaaatata aaaaggacat agagttatta ctaggtgttg aagtgggtta cagaaaacat
     2761 ctaaaaaatg aaatagaaga aataataaat tctaatcctt ttgattttgt attgtgctca
     2821 actcatacaa ttgacaatgt acctgttcca tcaaaggagt attttaaagg tcttagcaaa
     2881 gaagatgcgt attataaata ttttaatagc atacttgaaa ctaatcgtga atttggagat
     2941 tacaatatat atggtcattt ggattatata tctaggtatg gaatatattc agataataga
     3001 gttatataca atgactttaa agatgttatt gatgaggtct taaagtcaat aatcaataat
     3061 gggagtggta tagagctaaa tacttctggt tatagatatg gactaaatgc tatacatcca
     3121 aatgaagata ttctaaaaag atataaagaa cttggaggat ttatagttac tgtaggttct
     3181 gattctcata gggtagaaga tatatgcaaa gattttgatg tggcctatga tatgcttaag
     3241 tatcttgact ttaaatatgt atctttattt aaagaaagag aaacttattt tgtaaatata
     3301 gaaaaagtta aatctaacaa tatagcttag agtgtgtttt gaagtaaatt aatggatagt
     3361 taaaaagtta atagttataa tattgtatcc tctagaaaaa tactagaggt ttttattttg
     3421 ctaaaataac taaatagaat taaatttaaa tagtatagct tattttaaat tgaatagttt
     3481 attgttgata tattttacta ttatatattt ttattgtgat ataatagtaa aatatattaa
     3541 cctatcttta gattatataa attattataa aggagggaaa ttttaaaatg tctaataaaa
     3601 ataaagaaaa tataacttta agtaatcaaa taaaaggaac aattcaaaat agtttaacaa
     3661 aagaatctat attacatata ccaatgagca attatgccta tggttatgat agagaaacac
     3721 tacatataag acttagaact aaaaaaaatg aagcgaaaag agtaatttta cgaataggag
     3781 accaatatgt gtgggataaa ggcggtgcag gagtaggaaa tttaaatgca tctggagttg
     3841 gttggtctgg tggaacaaat atagtaatga gtaaagaagt agaaactgaa ttatttgact
     3901 attggatagc taagtgtaag cctttaaata aacgctcacg atatggcttt ataatagaag
     3961 gtcaagagga aaaactttta tttacagaaa aaagaataat agagttggga aatgaaaatg
     4021 atgaaaagga attatgtgaa ataggtaatt tttttggata tccatattta aattatatag
     4081 atatacctaa agtgccaaat tgggtgaaag atactgtttg gtatcagata tttccagata
     4141 gatttgcaaa tggtgacccg tctataaacc cagaaggagt agataaatgg ggagctattc
     4201 caaccagaga taattttaca ggtggagatt tacaaggtgt gatagaacac ttagattatt
     4261 tgagtgattt gggaataaac ggaatttact tttgccctat tacaattgga aaaaccaatc
     4321 atagatatga tacggtagat tatatggagg tagaccctac attaggagat aaagagactt
     4381 taaaaaaact aatagaagaa gcgcataaga gaaatataaa aataatgttg gatgctgttt
     4441 ttaatcatat aggatattat tctaaacagt ggcaagatgt agttcaaaat aaggaaaatt
     4501 ctcgatataa aaactggttt tatataaaag atatgagtaa aattgatact ccaatagaac
     4561 aaatagatga aaaaaatata ccttatgaaa cctttgggtg tgaaaaatat atgcctaaac
     4621 ttaatacaga aaattcagaa gtaataaaat atttattgga tgtaggcaag tactggatac
     4681 aggaatttga tatagatgcg tggagattag atgtttcaaa tgaagtagac catgtttttt
     4741 ggagaaagtt tagagaagaa gtcaaaaaag taaaaccaga catttatata ttgggagaaa
     4801 tttggcatgg tagtttacca tggctaatgg gtgaccaatt tgattcagtt atgaattact
     4861 tgatgtcaga agccatgaaa aaattctttt gtacagatga gataaacgcc gaagaattta
     4921 agtatatgat aaatgatgta atggtaagtt atccaataca agttagtgaa gttattttta
     4981 atttacttgg aagtcacgat actacaagaa tattgactta tgctaatgga aatattgata
     5041 gatttaaact tgcatatatg tttatgtttg tacaaacagg atgtccttgt atatactatg
     5101 gtgatgaaat cggaatggaa ggagaattaa gttcaatatc tgaaggtcaa agaaaatgta
     5161 tggaatggaa tgaagaaaaa tggaataagg atatatttga ttttatgaag aagattatta
     5221 gacttcgtaa agaaaataaa gaacttaggt ctatatcaaa tgaatgggtg ttagtagata
     5281 aggaaaatgg aacgattata ttgaaaaaag aatctatttc tataataata aataattcat
     5341 ctaaaaatat aattttgacg ttaccagatt atttagcaaa taaaaaagta aaagatttat
     5401 atgaagaaga aatagttgat ttaaaagaag aactaatgtt agagaaatac aagttcatta
     5461 ttttaaaata atacaaaaaa gtatttattt tagatttcat gttaaattat tatacagttt
     5521 aaataagtct ttataaacga taaaaaaata tggtctacaa gtgagtgaat tgaaagtaga
     5581 ttttaaatta aagactgagt tagagtagat aattttataa ataatatctt aaatacaaga
     5641 tagaaaataa attgattatt agtctaaaaa tttcttgtag aaagaggaaa taagaatgat
     5701 tgaagcattt atatttgatt tagatggtgt aattacagat actgcatatt atcactatat
     5761 ggcttggaga aaactagccc ataaagttgg gatagatata gacacaaaat ttaatgaatc
     5821 tttaaaaggc ataagtagaa tggagtcatt ggacaggata ttagaatttg gaaataagaa
     5881 atattcattt tcagaagaag aaaaagtgag aatggctgaa gaaaaaaata attactatgt
     5941 atcattgata gatgagataa cttcaaatga tattttgcca ggcatagaaa gcttgttaat
     6001 tgatgtaaaa tctaataata taaagatagg tctatcttct gcaagtaaga atgcaataaa
     6061 tgtattaaat catttgggta taagtgacaa atttgatttt atagctgatg caggaaaatg
     6121 taaaaacaat aaaccacatc cagagatatt tcttatgtca gcgaaagggc ttaatgtaaa
     6181 tccacaaaat tgtataggga tagaggatgc aagtgcaggt atagacgcta taaactcagc
     6241 taatatgttt tctgttggtg ttggaaatta tgaaaactta aaaaaagcaa atttagttgt
     6301 agattcaaca aatcaattaa agtttgaata tatacaagaa aagtataatg agtatatagt
     6361 taggagaatt atatgaaatg tttatttagt aatgtagatg agtggaagat tatacaagat
     6421 aagattgaaa tcaaagaaaa tagattagca gagtccataa tgagtatagg caatgggtat
     6481 atgggtatga gaggaaacta tgaggaaaat tatagtggag atacacacaa ggggtcttat
     6541 atagctggag tatggtttcc agataaaact cgtgttggtt ggtggaaaaa tgggtatcca
     6601 gaatactttg gtaaaattcc taattcagtc aactatatag gtgtaagaat tttcatagat
     6661 gatgtagaat tagatttagc aaaatgtaaa gtagaagatt tttatagaga attagatatg
     6721 aaaaatggta ttttaaatcg taggtttatt gtaaatataa atgataaaaa atttgaagtt
     6781 agggttgcta ggtttttaag tatatctgtt aaggaacttg cagtaattaa atacagtata
     6841 aaatcattaa attctaatac taaaataaaa tttgtaccat atttagattc aaatataaaa
     6901 aatgaagatt caaactatga ggaaaagttt tggaatggaa tttcttcaag ttctttagaa
     6961 tataaaggga gtattctttc tcgaacaaag gagaatgcat ttaatacacc agtattcact
     7021 gtgggttcaa tgatgtatat taagacaaat ggtgatgtag aagctatatc tcatgagagt
     7081 gattataatt actgtgaaaa ttcgataaca atggatgttt taaaaaatga ggttgcaagt
     7141 atagaaaaat atgtatctat aacttcaact agagattata aggaaagtga gctattaaga
     7201 aaatgtgaag acattttaaa tagagaacta tctaaaagtt atgatgatat attaaatgaa
     7261 catacaaagt tatggaataa aagatgggaa agtgcagatg taaaaataaa tggagataaa
     7321 agtagccaac aaggtataag atataattta ttccaactat tttcaacata ttatggagaa
     7381 gattcgcgat taaatatagg tccaaaagga tttactggtg aaaagtatgg aggagctact
     7441 tattgggata cagaagcatt ttgtattcca gtatatctag gtgtagcaga taaggaggta
     7501 actagaaatc ttttagaata taggtacaat cagttagaac aggcaaagga taatgcaaaa
     7561 aaactagggc ttaaaggtgc tttatatcca atggtaacat ttgatggagt agaatgtcat
     7621 aatgaatggg agataacatt tgaggaaatc caccgaaatg gaagtatagt atatgcaata
     7681 tataactata caaattatac aggtgactat aaatatataa aagaaaaagg aatagatgta
     7741 ataatagaaa tagctagatt ttgggcaagt agagttcatt tgtctacaaa aaaagatttg
     7801 tatatgatac atggagtaac aggtccaaac gagtatgaaa acaatatcaa taataattgg
     7861 tatactaatt atatagctaa atggtgttta gattatgctg tagaaaatat cttacgatta
     7921 gagaaagagt gtaatgaatg tattgacaga aataatgtga ctagggaaga agttgagttt
     7981 tggaaaataa tatcaaataa aatgtatctg ccgtatgatg aggaactaca aataataatt
     8041 caaaatgata attttttaga taaagaattt gtagaagtaa aggatttacc aaaagaaaat
     8101 tttcctctta atcaaaattg gtcttgggat aagattctta ggtcgtgttt tataaaacaa
     8161 gcagatgtat tacagggaat ttactatttt ggaaatagat ttactaaaga agaaaaaaag
     8221 agaaattttg acttctatga aaaatatact gtacatgaat cttctttatc ttctagtatt
     8281 tattctatta ttgcctcaga aatagataac ctagaaaagg cttatgaatt atattcaaga
     8341 acagctaggc ttgatttaga taattacaat aatgatacaa atgatggact gcacataact
     8401 tcaatgtctg gttcatggct ttccattgta catggatttg caggcatgag aacatggaat
     8461 gaaactctat cttttgaccc taagttgcca aaagagtgga aaaattattc tttcaatata
     8521 aattatagag ataatattat taatgtagat gtatgtagtg atttaattaa aatagaaaat
     8581 ttaaaaggta aaccaataaa aattattgta tatgggaaaa aatatatatt agaaaaatgt
     8641 ataaaaattg ataaatagat ttaataatat ttttattata taaatagtta aaataagcta
     8701 tctaattatt ta
//
LOCUS       DAEMHZ010000152         8548 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000152 DAEMHZ010000000
VERSION     DAEMHZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.153"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    355..592
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            656..2575
                     /gene="thrS"
                     /locus_tag="KPK72_003277"
     CDS             656..2575
                     /gene="thrS"
                     /locus_tag="KPK72_003277"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG2490468.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3343..4458
                     /locus_tag="KPK72_003278"
     CDS             3343..4458
                     /locus_tag="KPK72_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2490469.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETETRVARETAIRVAKEKDY
                     KIIIMLKKNGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(4678..5715)
                     /locus_tag="KPK72_003279"
     CDS             complement(4678..5715)
                     /locus_tag="KPK72_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG2490470.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFVSLFIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNIVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITDLYLSTT
                     NGNLTIIKNNH"
     gene            complement(5708..6394)
                     /locus_tag="KPK72_003280"
     CDS             complement(5708..6394)
                     /locus_tag="KPK72_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490471.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGVAGINIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(6406..6726)
                     /locus_tag="KPK72_003281"
     CDS             complement(6406..6726)
                     /locus_tag="KPK72_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490472.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            7087..8427
                     /locus_tag="KPK72_003282"
     CDS             7087..8427
                     /locus_tag="KPK72_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2490473.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         3235 a          913 c         1394 g         3006 t
ORIGIN      
        1 gtgttactta ttaatagtat ttaagtattt actgtactac ttattaacta tattaagtat
       61 tgattgtatt taagtataca aaacctaaaa agagtacaaa aattagaagt tagttttaat
      121 aaaaattagt tttagtattt atctagtcat atctatcatg catgtggata cgttttaaaa
      181 attttaaatt tatgaaaata ttataatatc actataaatc aagagattaa tttaatataa
      241 aaatataaga atattggttt acagctgatt gagatgaaaa ataatgggta attaatatat
      301 tacattaatg ttgattgatg tataactatc tgttataatg aaaatatatt aaaaatcata
      361 gtgttgaaga ggaagagtag agtagaaacc attctcagag aatttgggtc tatggctgag
      421 agcccatgta ataggtctat ttgaaaacta ccttggagct gagcttatta gcagtatttg
      481 ttttgtacta tacgtgaaag tgctcgggtg acgaccttat agtcatcaag agggttgtat
      541 atacttgtat acgccaactt gggtggaacc gcgaattaat atcgtcccaa acatatttgg
      601 ggcgattttt tattacaaaa aatctaaaaa ttatttcaat aaggaggaaa aaacaatgat
      661 taaagttgct ttaaaagatg gctcaataaa agaatttgaa aatgctattt ctgttatgga
      721 tgtagctaaa tccattagtg aaggattagc tagaaatgtg gtagcagcgt ctgtgaacgg
      781 agaggttgta ggtctggacc acattataga tactgattgt gacttgaatt tatttaagtt
      841 tgaagataaa gaaggtaagg aagtttttag acatacatca gcacatatat tagctcaagc
      901 tataaaaaga ctttacccag aagcaaagct tgctatagga ccaagtatag aaaatggatt
      961 ctactatgat atagatttag accatagatt agttccagag gacctagaga aaatagaagc
     1021 tgaaatgaaa aaaatagcta aagaagattt aaaaatagaa agatttgaac ttcctagaaa
     1081 tgaagcttta gagcttatga aagaacaagg agaagactat aaggtagaac ttatatctga
     1141 tttacctgaa agtgaaataa tatctttcta caagcaaggt gattttactg atttatgtag
     1201 aggaccacat ttaccatcta ctaaaaaggt aaaagctgtt aaattacaaa gtgtagcagg
     1261 tgcatattgg cgtggtgacg aaaataataa gatgcttcaa agaatatatg gaacttcttt
     1321 tgagaaaaac aaagacttag aagagtactt acatttacta gaagaagcta aaaagagaga
     1381 ccacagaaaa ttaggtaaag aattaggatt atttatgata cctgaagaag gtccaggatt
     1441 ccctatgttt ttaccaaaag gtatggaact taaaaatgaa ttattaaaat tctggagaga
     1501 aatacacaga aaagctggat atattgagat agaatctcca atcatattaa atagaaaatt
     1561 atgggagact tcaggacact ggtatcatta caaagaaaat atgtatacag ttaaaataga
     1621 tgatgaggat tatgctataa aacctatgaa ctgtcctggt ggattaattt actataactc
     1681 tcaactacat tcatatagag atttcccaat gagagttgca gagcttggta gagttcatag
     1741 acatgagtta tctggtgcat tacaaggatt aatgagagtt agagcattta ctcaagacga
     1801 ttcacatata ttcatgttac cagagcaaat taaggacgaa ataaaaggag tagctaactt
     1861 aatagatggt atctacaaaa cttttggatt tgaatataat ttagaattat ctacaagacc
     1921 agagaactct atgggaagcg acgaagagtg ggaagcagca gaaaatggat taagagaagc
     1981 tttagaagag ttaggtctac catatactat taatgagggt gatggagcat tctatggtcc
     2041 taagatagac ttccatctaa aagattgttt aggaagaact tggcaatgtg gtactatcca
     2101 attagatatg caacttccaa gacaatttga taatacttat attggtcaag atggtgaaaa
     2161 gcatagacca gttatgattc atagggttgc ttttggtagt atagaaagat ttataggtat
     2221 attaatagag cattatgctg gtaaattccc agtttggtta tctccaactc aagttaagat
     2281 acttcctata tctgataaat ttatggatta tgcaaatgaa gttaagaaag agttatttga
     2341 taagggtata agagttgaat tagatgatag agctgaaaaa attggattta agataagaga
     2401 agctcagctt gaaaaagtgc catacatgtt aatagtaggt gaaaaagaag tagctgataa
     2461 taatgtttct gttagatcaa gagataaagg tgaaataggt agtattaaat tagatgaatt
     2521 tatagctagt atttcaaaag aaattgaatc aagagaaagt attattcaag actaatatat
     2581 attattttag tagctaagat attgttatat aagataagtt taatcaatat gttgatatgt
     2641 tgatgcatag tgattaaatt tatgaaaaga tgagtataat tagaggtaca gttaatcata
     2701 tttttatgat taatgtacct ctagtttttt gtatggtaga tttattaata aatatatcat
     2761 agaagtgttt tgaacatgga aatatttatg tgcattcttt aaataaaagt ttattttttg
     2821 agtgataatt aagtgtttgt tggtaaggtt atggctgtaa aaatcataac tttattttca
     2881 tatttgttcc attttcataa aaattattat ccctttaaga aaatttctta atcctcttga
     2941 aatatttcct ttttagtcta aagttaaatt tctttcttca aaatgaatat ttaaatgaac
     3001 taaaatttaa gtacattttc taatcttatg agtctatttt tcatagataa ttgccctata
     3061 atagaagaat ctatgttgga gctatttggt gacaataaca gcattgtcat tgattaatta
     3121 atgctttata aatgattaat tagtgtttta tgttattttt ggacttatcc tctatacata
     3181 gattaattta tttaacttta gatatagaga gaatgtcaag tacatacaca gacattagag
     3241 tttaagaaag tgtttatgga gcaagaactt ttaaaagagg tagtgactta gtttaatttc
     3301 aagaaatgat aaatacaaaa gaggaaaaag agaggtgttg aaatgataga aaaagtagaa
     3361 gaaaaatcta agattattaa aacatcaaaa gaatataagc gaactataat tgaagaaaaa
     3421 gataaagaat ggaccaaaaa attaaatgat atacttagta gaagtagttt ttctaatgat
     3481 gaattagata aaatcaaggc gttaattcca aaagagattt tgactaatga aaatgttgga
     3541 gaagttgtga ctgaagatgg atatgctaag cccgagtatg atgaggtatt taaatcattg
     3601 ttcactttaa ataatgaata tgatttactt gtaaatttta taaatgatat tctaaaagat
     3661 gctccatatg ctaacaggaa tatcaaaccg ttcactcaaa taaagagaat aattagagtt
     3721 gaaactgatc caacaattaa ttacattgga gagaaaagac ctagacttga tattttggct
     3781 gaagatgaag aaaataatca tataaatatt gagatgcaaa gagcttttga gaacgattat
     3841 ttggaaagag ctgaatatta tcttagtcgt gtacatggta gaaagttaga agaaggtaaa
     3901 gagtataaag aaattggaaa aactattgga atacatatac taaatcatgt aaaatataat
     3961 cacatagaag attatgtaaa ttgcctgaga ttaacaatgg acgggcatcc agatatttat
     4021 tctagtaaaa ctgcattata ttttatagag cttccaaaaa ttcataaatc tgactatata
     4081 gtaaatagaa ttatgctatg gggaaaactt attagtaatc cttctaactt agatgtaaga
     4141 gttatagcta agaatgattc tattattaaa aaagcacttg atagattaaa agaattaggt
     4201 agcaatgaag aatatttatt aaatctgaaa agaggtgctt atatcatgaa taaaagtaga
     4261 aattttaaag aagagattga aagagaaacg gaaacaagag ttgcaagaga gacagcaatt
     4321 agagttgcaa aagaaaaaga ttataaaata attataatgt tgaaaaaaaa tggttttaaa
     4381 ttagatgaca tattaaataa atttaatgat tttgacttaa gtgaagatga gataaaagaa
     4441 gtatatgaaa ataattgatg ataaataaaa agtgaactta tataagtttg ctgaatcgga
     4501 gtctagaaat atttatgtgt attttttaat gaatgaaaat ttagtttaaa aacaataatt
     4561 tagaggtaca gttaattata tttttatgat actgtacttc tagttttttc tattgtagat
     4621 ttattattta ctaataaatc tacaatagaa gtgcttttgt tgtaagaatc attttatcta
     4681 atgattgttt tttataatag ttaaatttcc atttgtagtg cttaaatata aatctgtaat
     4741 accattttta taagtgtata aatcactatg acgattcaac tcaaagtcac tgtccaaaac
     4801 accagaaacc ttatcaaaat ttaaagtgaa acctggatta tcaggaatag acaaatcgac
     4861 attggcagaa gtagtttcac agtacagaga agacaacatt ttgctagaat tgatttttat
     4921 attactgcta gttgactcag ctttaatgtc agaaaaaact ccagataagt tcatttttcc
     4981 agaaactgaa ctacaagtaa gatttttatt gtatatatct tttaaattta tattgccatt
     5041 agttgtttct aagtttaaaa catcaatttt cccagaaagc tgtatttcac cagatgttga
     5101 tttgagtgta gcatttttac tatttagttt tgttatatca atgtgaccac tagttgtgtc
     5161 taaatttatt ttattaatat taccagagag acctattgca cctgaaattg tgttagcttc
     5221 cactatatta cttcttatgt aagtgctata tatatctgct gaagtagtat ataaggttat
     5281 tttatcataa agcttattgg gcaaatatag ttcaagtatt gttggttttg gaagattaat
     5341 tccaatgaag aattttttag ttgaattatc atatatagaa agagaatttc cgtcaaccat
     5401 tatttttgct agttttttct ttgagaaatt actagggatt ttttgaatta atttgatttt
     5461 attagaatca cttctcttaa ttctaacttc acctgaaatc caatcaacat taatgtcatt
     5521 tattttatct gaaaaacttt catttttagc cactatatag ttatcgtaat tgtatttagg
     5581 attaagagta ttaaaaggga aattaaaaga atgtgaaatt atagttccta taaagagtga
     5641 gacaaataca atagaaagtg atattgccag tatactccag atgaaaatct ttgcctttat
     5701 aagcttttta ttcatattta tcctcctcct tgtagccttt tagttgtata attcctttga
     5761 caatgttgat accagcaaca cctattataa atagtatcca cgaaaaggac caaatattaa
     5821 atagtatgct tgtgattaga tatatagaaa ctactataat ccaatatgca ttcataaatg
     5881 atttataaac tggatttata tgattataat tttcctcaaa atcctcaaat tctttgaatt
     5941 tatttgataa attatcattg tatagagcat agtttgtcct tgtcatttga ttatagacta
     6001 ataaacctac tccaagtgct attaatatga gcattaaaag aatagctata gttggttcta
     6061 tgccaaaact tacaactgag actacaaata ggaaggggct tattataaaa agagcaactg
     6121 ctattgatgc tcttaaggct cttttctttt tttcttcaat taattcagaa ggtagagaat
     6181 atggttcctt aacactttca attaactcag ataaatcacc aattcctgag attactgagt
     6241 tatatgcttc ttgttctgtt ttaccatctt gtatttggtc gttatacttt tcttttagat
     6301 tttcaaataa ttcatcttta agttctttga ctttttttgt ctcattaact cctgtaaagc
     6361 tactgtcaat ataagtacgt attttgtcta acatattttc acctcctaaa ttaatatatc
     6421 tatcaatgct tttgtattat tccattcttc acggcttttt atatatttct ctttacctat
     6481 gtttgttatt gaataatact ttcttctagc tccagtaact tcatcacccc aataagaggt
     6541 tattgcacca gattgttcaa gtctacgaaa tgctgtatac aaagtagctt ctttcaactc
     6601 atacaatcct ttggtttttt caagtataga tttattaatc tcataaccat aactgtcttt
     6661 ttcaagtaaa tgttttaaga taattgtttc tgtatgacct cttatcatgt cagatgcgat
     6721 tatcatgaac atcacctcca attaaatata taatactttg atatacttcg cctgtcaagg
     6781 tatatcatga gaaaagacat tttattgtaa tatttataag attttatata gaaataatgt
     6841 aaaaacttta taaataatat attttatacc tagttataga taaatttata tagttattat
     6901 taaaaaaagc aataaaaagt ttaattcaat ttagcaaatt tagtgatata attaaaaaag
     6961 aaaagaaata tggacttatt tggaataatt ttgacaatct atttactaca cgtgtagagt
     7021 cgtatataat taaattatac ataaaatata aaaaaataat ttagtaatac gttaggagaa
     7081 agtattatga taaagtcaag cttattttgg aatattttca ttgcgatatc ctttatgata
     7141 gaatggtttc tttgtaagaa aatgctagat tatacaagtg agaaaaggat ttctaacaaa
     7201 aaaataaact tttgtgtggt aggtattatt gtttttgctg acataatata tttttttgat
     7261 gtacctccaa atataagagt aattatatct atgataatta ctattgcatt ttataaaatc
     7321 tcatatgatg tggggtttat aaagtctgta ttaatatcct taatttattg gatgttacta
     7381 atcgccacag atgctttgag tatatcttta acagtttggc ttaattctat aaatgacatg
     7441 agtagtatgg ggacgggaga tatatacaga attgagtcta taatattggg aaagatactt
     7501 ttgattgtgt ctttgctttt atatagagcc ttaaagataa aacttaatat agaaataaat
     7561 aaaaggacat tattttatat agttattcct atagtcgcta atatagcatc tttttttgta
     7621 atttttgaat acatatttaa tccagggtct ggtagcttaa tacataattt ccaattcgtt
     7681 atgatttctg ttgtattgct tatatcaagt atcactttag ttttaattgc taaaaaatta
     7741 cagcaatata atactttagt tgtagaaaac aatatgatta aaaatacttt gaagtatgaa
     7801 aatgagtatt atgaagatat agaaaagaaa tatttaaaaa ctagattact aagtcatgat
     7861 atgaaaaatc acatattgtg tataaatgct atgattaaaa aaggtattga tgttacttct
     7921 tatttaaata atttgcaggt agaaattcag agtaatgact tgttatttaa tactggtaat
     7981 tttatattag atgctatatt aagtgaaaag aaagaggttt gtgataagaa tgatatagaa
     8041 ctaaaaatag gaataaattt tagttgttgt gaagctgttg aattagtaga tatatgtcat
     8101 atattttcaa atattttaga caatgcaatt gaggcatgta ataaaattga tagtggaaat
     8161 agagttattt gtgttaaagg tgatgttatg caaaattttt atttaataag agttgaaaat
     8221 actaagatga ataagattat tactaaaaat ggagatattt tgacagacaa aaaaggttct
     8281 cttcatggac ttggaataaa aagtgtaaaa aactctgtaa ataagtatgg tggtaatgtt
     8341 gtaattgaat atgatgataa aaaatttgta gttaaaattg ctattccact tagaaatgtg
     8401 aagaaagcag ttaatcatgt gatttaaatg tatataaatt tgatgtgtag aaatttaata
     8461 tataagaatt aatgtataaa agtgatgcat agaagtttaa tatataagaa ttaatgtata
     8521 aaagtgatgt atagaagttt aatatata
//
LOCUS       DAEMHZ010000153         8497 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000153 DAEMHZ010000000
VERSION     DAEMHZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.154"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(153..329)
                     /locus_tag="KPK72_003283"
     CDS             complement(153..329)
                     /locus_tag="KPK72_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490474.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(433..1041)
                     /locus_tag="KPK72_003284"
     CDS             complement(433..1041)
                     /locus_tag="KPK72_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2490475.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITTREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            complement(1140..1964)
                     /locus_tag="KPK72_003285"
     CDS             complement(1140..1964)
                     /locus_tag="KPK72_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2490476.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(2142..3035)
                     /locus_tag="KPK72_003286"
     CDS             complement(2142..3035)
                     /locus_tag="KPK72_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2490477.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(3292..3729)
                     /locus_tag="KPK72_003287"
     CDS             complement(3292..3729)
                     /locus_tag="KPK72_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG2490478.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(3986..4135)
                     /locus_tag="KPK72_003288"
     CDS             complement(3986..4135)
                     /locus_tag="KPK72_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG2490479.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(4148..4378)
                     /locus_tag="KPK72_003289"
     CDS             complement(4148..4378)
                     /locus_tag="KPK72_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG2490480.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(5238..5459)
                     /locus_tag="KPK72_003290"
     CDS             complement(5238..5459)
                     /locus_tag="KPK72_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG2490481.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            6039..6296
                     /locus_tag="KPK72_003291"
     CDS             6039..6296
                     /locus_tag="KPK72_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2490482.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            6765..8003
                     /locus_tag="KPK72_003292"
     CDS             6765..8003
                     /locus_tag="KPK72_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2490483.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         3201 a          959 c         1123 g         3214 t
ORIGIN      
        1 atataacaaa atcctataat aaataagatt ttacaatata ataaaaatcc tataataaat
       61 aggactctgt aatataataa aaatactata atatagtaaa aatcatgcaa taataaaaat
      121 ctataataat aaaccccagc ataacaaaga cattaatttc ttactaaaaa ggccaccaca
      181 attaacagtg aaaaagcaat tgaaagtatt gaaaaagtaa agtgagaatt agagtcatta
      241 ttacgattat taagttttat ccataaacca catataattg tacacaacaa taaaattgct
      301 gatattactc ctataaatgt aaaaaacatt aaaattcctc ccccttattt aatgcaagta
      361 tttttagtca atgagaatat tttcaaacgt taccataaaa taaaaaaatg tacataaaaa
      421 attaatatag tattattttg taatatctac caataataaa tctaattgaa aatctattaa
      481 accatttctc tgttcaacat taagaatatc aataccacta tacttgaaaa aagcattaga
      541 acgaagaaaa agcaactgaa taaatgaagt tatttgatac gcaatagttc tgtattcagc
      601 ttcagttttt ttgttattct gattttcata gtgagcctta attaaaggta gtatctcaaa
      661 tggataaaca atttcttctt gtatcaatat atagggtaca gaaatcttta taaattggct
      721 atgttcacct ataaaatcaa aaaatgcaat tagtatttcc cttaattgct gtttaggtgc
      781 taaattttta gtatcatgtg tagccttata ttcatccata tgacgcttta aaaatttttt
      841 aatagaaata ttaattaact cctctttaga tttaaaataa taattaatca tagctaaatt
      901 gacatttgct ttagctacaa tttctctagt cgtaatgctt gaaacatctc tatgtgaaga
      961 tagtaactgg attgtggcat taattaattg ttcctttgtt gaatctgctt tcataatttt
     1021 tccccttaaa agtttctcca taaaaaaatt aaacatgttt aatacacgtt tagatactat
     1081 catgaaaatt ttgggatgtc aatgggtgat tagtacttaa gtatttaagt actaatcgat
     1141 tagactttta taagggggat ttgaatttct gtaagataat tttcaggatt tatttcatta
     1201 tgctcaccct tatgaccgat ttgtctattt atacctgata tttgataatc actattttta
     1261 tctaaccaat aacagaacga ttcatatgca ccagctatat tttcatatgg accataaacc
     1321 atagtacatg ccatcatatc aatttcttct gtctcacgat atataaatcc agatttgttc
     1381 tttccaatct tctttacaac cattccaact tcaatatcta caccattatc tttgacttct
     1441 tcatcatggt aaaaagctat attattgttg ggttgacgtg atacttctat atgttcttct
     1501 ttaataaatt tagataactt ttcccagagt ataccttcag attggtagtc tgggactacc
     1561 tctctaagtg atatgatttt ataacttgga atctttttaa agacaacatt gtaatgaatt
     1621 tcatctttat cacagtttat agcttctata aatttatcaa ttttatttat tctttgttgt
     1681 tcgtgcttta tttccttttg tatttcattc ttttttctat tcaattcttt aattacaaat
     1741 tcatcattcc aattatgtac gatatttgct atttctgcaa cgctaaattt tgaatctctc
     1801 aataatacaa ttctttgtaa tattgaaatc tgttcaactg agtataatct atgacctgta
     1861 tatttgtcta tttctgcagg ctttaaaata ccaagttcat cataataacg caacatacga
     1921 atagaaattt gtgttagttt tgaaacctct cctattttaa acatgaaaat atgacctcct
     1981 tttcacttat aattagtata tattttgttt ttatatactt caagcgattg tggaaataca
     2041 attattttcc agatgttgca ataaattata gagagtaaat tatatttaag tagtaatcgt
     2101 atcaaatcat agattatctt aatttataat tcatattaaa attaccagtc taactcaaat
     2161 tcaaaatcat ccgactttat ctgttcaatt tcagaattta ccttctgttt aaatttatta
     2221 gaagaaatca tatatacaag aaatattaaa ccaaatatta tttgtacata ttgagctatt
     2281 acacataatt taatagggta gtgtagaagt aattgaatgt tgaaaaaata gtacatcata
     2341 tgaactaacc cagctgataa aatgcttcta cttgtattga tatatacaaa attaattata
     2401 aagtttaaaa gtacaatgtt tggtataaat aaaaaagcat caaataggga gtattgtgcc
     2461 aaattatatg gtatttgatt aggtgtaaaa tacgacccta gataccagat tccacaaaca
     2521 agccctaaaa taattgaaga gcctataaat ccaaatctaa ctaataattt gtcaatggcg
     2581 tatcctctcc atccagcttc ttgactaagt atactcaata taaaacttgc taatagcata
     2641 taagctattt tagaaggtct aaacactgct atgtgtatcc aatctatatc agaagttttt
     2701 acattaaagt aatttccgat tattaacgaa gtaataaaag ttaaagcaaa aaataatatt
     2761 gttaatagta tccattttat tctaatttct ttaaagctta ggtatctata aataaaatct
     2821 tgttttgcac tttgtggata gattgtagaa ataaaaaata gacctacaat agctggtgat
     2881 gtatatgcaa gataaaaaat gatttctcca agtattgtat ttgtaattcc gagcaacgca
     2941 ggtgtaagac tgaatatcca gttcaatact agcgtagatg taaagaaata gattaagtgt
     3001 ttgtttgtat taaagggggt gttatatagt tgcatttttt cactatcctt ccgttttata
     3061 atgaagtttt aatttttatt aattgtttat ttatttttat atgtattaag ttatcgcttg
     3121 atgttagtta ttgcctataa taatatccta ttttttatat tttgattaat tatttttaca
     3181 ctaaatataa ttaattagta tttaatattg aacatattat attattatta attattaaat
     3241 aggtcaataa atttgcaatt agtgatatta ataaattact ttagattttt attaaatgtt
     3301 tagcttttct ttaatatttt ttatataacg acgatttata ataacttttt cgttgttttt
     3361 taaagtagct tgcatgcgat tatgtgatat tacttttata ctttttatgt agtccatatt
     3421 taaaatcatg gatttagata ttcgtgagaa ggatgtatta gctaataatt ttgataattt
     3481 atatattttt aaatcaattt caaatacatc atcttcataa taagcaaatg ttttatttcc
     3541 aactgtttca aagtataaaa tatcacagac attgagttta taaatctctc ctgatttttt
     3601 accttttata tcaagtaatg gtgtactcaa taatgaaatt attttttcaa tgttattatc
     3661 tagagcagaa cattttatgg ttatttcaat atcatcacaa ttaatacctt gttcgacatg
     3721 tattttcatt aatcatcacc tcattttatt actgtcatta gaatagcatt atatcttatt
     3781 gagtccaact agatgcgttc taaatttcaa attaagcata gtgaaattca aaaataaaat
     3841 agtgaaattc aaatttgagg tggtaaaatt caaaaaaata ctatggatat ttggataaat
     3901 tatccaaata tcctagtatt tagcatagaa tatgtctaat tattttacaa atagacctat
     3961 tttatgactt acatatattg tcaaattact tcttaaatat caaaatattt ttggataatg
     4021 tatcaatact gtttgataaa gtatttaatt taccctcaat tctaaccaac aagtacatgg
     4081 atagagctat aggaaaacca acattggtta ttaacagttg taaactatca tccataaaat
     4141 tagcccccta ttctataact aagtcataag gagtggtatt tgtttccaca accttggctt
     4201 caacaacaga agctaggtca aatcctccaa ccgaaaaaat atttttagat actatcaaat
     4261 ccatgacaga ttttatattt tcttcagtta aatcatcctt tggattttct atagaaaaag
     4321 aaacttgttt atcctttaca tttttaaagc tcatgactaa ttttttctta atttccatat
     4381 aattacctcc gatataataa tttatatttt aattgtttgc ttgttaatat tggtatttta
     4441 attatcaata ttaatgtttt gcttgttagt attaatattt caattgtcaa tattaatgtt
     4501 ttgcttgtta gtattaatat ttcaattgtc aatattagtg ttttgtttgt tagtatcagt
     4561 gtttcaattg tcaatattag tgttttgctt gttagtatta atatttcaat tgtcaatatt
     4621 agtgttttgc ttgttagtat taatatttca attgtcaata ttagtgtctt gcttgttagt
     4681 attaatattt caattgtcaa tattagtgtt ttgcttgtta gtattagtat ttcaattgtc
     4741 aatattagtg ttttgtttgt tagtatcagt gtttcaattg tcaatattag tgttttgctt
     4801 gttagtatta gtatttcaat tgtcaatatt agtgttttgt ttgttagtat taatatttca
     4861 attgtcaata ttagtgtttt gcttgttagt attaatattt caattgtcaa tattagtgtt
     4921 ttgcttgtta gtatcagtgt ttcaattgtc aatattagta ttttaattgt tgactctagt
     4981 tattgctgat atagaaaata tttaatttat ttcaattaat tagattaaag tagggtagca
     5041 tctaaaatag ctaacatgtt taagttgaaa tacgtttaat atattaattt ttcaaagcgt
     5101 taatatatta aagttataac caaaattgaa gttataatct gccaaatcta tagcgtatta
     5161 agtgcgttat ataacttaat cttttatata aagagttaat ttttttaatt tgtaaagcag
     5221 agtacgttta aaagatacta tttagataat gatgtactat ctaattttaa aatatctaca
     5281 atagggttct cttgaagact tgctattgct tctgcaacag cataaacatc ttcatttaat
     5341 gcctctgctt ttacatttgc aaatgattta cttttaacaa ctgccttacc cttatcatca
     5401 aaaccagcat ccaattttag ttttatacta gataaatttt ttgttgaaac aacttccata
     5461 aaattacctc ctaaaaattt aatttcaaag atttgtcgac tttctttgtt agctataata
     5521 ctatcatcta agataatatc ggagttaaac agtaatttct aaagattttt tgtgtaaatt
     5581 tttataggaa aaattttact tttttgctga tgaaaaagtt tacaagttat ttgtgaagat
     5641 atgttcttag tgttttgaaa tagtatttgt atatattcat gcttagttga gatagttttg
     5701 tatggatttt catatgtgta gagttaaatg ttaggtacta aagtttatgt taaattaaat
     5761 gttaaagctt atgttaaaaa ttttattaaa tttaatctgt aaataaaaaa gttaaaagtc
     5821 caatattaac attttacttt taaaccatta aaatctttat taaagctaac aaagttataa
     5881 gttatgagaa attttattga agttaataca aaaattaatt ttaaagctaa gaaatttttt
     5941 attagattca ataaaactaa taaatatagt ttagtcaata atgtattaaa taaaaacttt
     6001 gttaagtcta taaaaatatc ttaaaggagg tgtaacttat gaagataaaa gtggaggata
     6061 taccagttca atttcatgca atggtagaaa taatcggaat tgataagttc atagaggttg
     6121 ccaagttgta tggaggaaca aatacttata tacctacgta taaggggctt tttagatatg
     6181 ctagaaatag ggatatagta aaacaattta atggagtcaa ccacaatgaa ttggctatta
     6241 gatataatat gtgtgtaagc aatattaagc gaatactcaa tgaaaatagt gtttaaatat
     6301 aataaaataa tgtttaaaca taatgaaaat agccttgtta aacgtaagag taaagacaaa
     6361 gaagatgttt tataaagttt ttttagacaa attaactaaa tttactactt attaatttaa
     6421 atacattgta tattctataa aaaaatttgt taaatactgg agatatattg agaatacaaa
     6481 gaaaatggtc gaaaatattt gttggctaaa aaatagataa aatgatacaa ttgcttgtat
     6541 aaaggggaaa aataaataaa taaactatat aaataaagaa atagcaatta atatgacata
     6601 atatatttat aaaattaaga atataaatag atattttaag ggattagttg ttaaattagg
     6661 aaatattaaa aaaaatatat atttatattt aaactatttt gttatttata taaaaatact
     6721 tatctagtta ctagcaatta ttagtagaaa tgggggaaat agagatgaat aagggatttt
     6781 acaaattaaa ttcagtggta ttgaactttg atattaagaa tgatgttaac aagataagat
     6841 ttaatcatta tataatgaga aaagaaaaca tacaaagact tcaaggaact ctgccaaacg
     6901 gacaatttta tatgacagtg agaagagctg ctacagattt acaactttct atttctacta
     6961 taagtaggct tgtaaaagag tttttggatt tagggataat aagattagtg tctaagggtg
     7021 taaaaggtaa tcgttctgta tatagttatg taagtataga gtttaatgga agtttatttg
     7081 atgatagcaa atatatggaa cttgactgtg tatgtaatga tgaatttatt gataatagtg
     7141 gcatagagtt tgattgtgat agcaaagata attcttttaa tggagatata aatgtagaaa
     7201 ctgtgcattg cattaatgag tatttgtctg atgatgatgt agagcataat tataacaatt
     7261 tccataacaa agatactata aaggataaag ccatttctaa tattagcact atcaaaaaat
     7321 atattgatga atcttttgat acaaataaat cagaaaattc taacttacaa caggagtcaa
     7381 taaatttcaa ctttaatgat acatgcaatg aaatagataa aagaacgaat aatagtatag
     7441 aaaataatca aaataaaagg cctatgagat tgagtgacat cagtgtttta agtgataaag
     7501 gtatgacaaa taaaaagaat tactttaatc ataatcatgg tatacaaaaa gggactaaat
     7561 ctgtaacaaa gaaaaaagaa tccttaaata aatttaataa aaaaaatata cagcaaagca
     7621 ttataaatat gctaaataaa aaatcaggta agaattttaa aatcgattct ccaataagca
     7681 taaaacttat caatgaaagg ctaaaggaag gctatgtctt agaagatttc tacaaagtta
     7741 ttgaggtaaa agtggctaag tggaaaaata ccataatgga aatgtatata agaccagaaa
     7801 ccttgtttag ctataagttt gaatcctatg taaatgaaaa catatctatg aaaagtcaaa
     7861 gtaatgttca cccatgtaaa gaaggcaata gtaatgatag cagtaattat atacattctg
     7921 attatacaac atatgatggc agtgtgcgtg agagaaaaaa atatgagaat gtaagatact
     7981 ggaatcctat atgtgagatt taattagttt aagaatgtat ttatgataaa taagatatta
     8041 atagagaata gtgagctata gcaaatgata tatcaatagt tggtagtaag ttatattaag
     8101 caagatatta ctatcaggta gtgagttata ttgaacgata tatcaataat tggtagtaaa
     8161 ttatattaag taagatatta ctatcaggta gtgaattata ttgaacgata tattaataat
     8221 tggcagtaaa ttatattaaa taagatatta ctatagagta atgaattata ttgaacgata
     8281 tatcaataat tagtagtaag ttatattaag taagatatta ctatcaggta gtgaagtata
     8341 ttgaacaata tatcaataat tgatagtaaa ttatattaag taagatatta atatcgagta
     8401 atgaattata ttgaacgata tatcaaattg gcagtaagtt atatcaaata agatattact
     8461 atcgagtaat gaattatatt gaacgatata ttaataa
//
LOCUS       DAEMHZ010000154         8479 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000154 DAEMHZ010000000
VERSION     DAEMHZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.155"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..577)
                     /locus_tag="KPK72_003293"
     CDS             complement(<1..577)
                     /locus_tag="KPK72_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HBG2490484.1"
                     /translation="MLKILQEKNVRMIWSKDGSEVWFHAGDVGEELGVIQINSTLRNI
                     DKEYKKKFKNSDMHEMHIRNFDKALNNKGEIFITEEAIYNISFRSNKAEAKLFTKWVS
                     KALKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATNQGSNKPQMYY
                     KHFTELVRKKLGIPKDVKRDELNQSELFDIQA"
     gene            complement(1110..2252)
                     /locus_tag="KPK72_003294"
     CDS             complement(1110..2252)
                     /locus_tag="KPK72_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2490485.1"
                     /translation="MKVINFDPIKERKIVPSKLKQARISRNLSLAQLSKLIGVSSQTI
                     SLYEKGKIQPSSPVLIKIIDELDFPINFFCENEEMDLSDGTVYFRSNKNIPKKIKDAC
                     KVRIAWINSAYKLINSYFNLPKIDIPDFGDISFDSIDDLKIEDITIELRRYWRLGESP
                     IENLIDLLQQKGIVITKLEIGNKKIDAFSKWESEIPYVFLGSDKDSAVRSRFDLAHEL
                     GHLILHRNIDEEDLEENINLIEHQADWFAASFLLPLRSLDKEVLSSSIDNFVLLKKRW
                     KVSISAMIRRCRECDILTENQIKYLHSQMIKNRYYKREPLDDVIVSEKPYLFKQAFEI
                     LVENDIFTKESLLEEIKLNKQEAISLYGLDKDFFDKKTDFLKLIEF"
     gene            complement(2433..2921)
                     /locus_tag="KPK72_003295"
     CDS             complement(2433..2921)
                     /locus_tag="KPK72_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490486.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPKIKENELPLF
                     KN"
     gene            complement(3080..3292)
                     /locus_tag="KPK72_003296"
                     /pseudo
     CDS             complement(3080..3292)
                     /locus_tag="KPK72_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(3319..3930)
                     /locus_tag="KPK72_003297"
     CDS             complement(3319..3930)
                     /locus_tag="KPK72_003297"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013826.1,HMM:NF024848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG2490487.1"
                     /translation="MPSKYYAVRKGHKIGIYNNWEEAYKQVRVYSCAEYKGFYSLKQA
                     KLYLEGYNLGLEDIKQDKDTMIAYISGSYSEKLNKYGSGVAICRPNGMVYEEWDNGEN
                     RTALLLKDIAGEIKAAIIAMREIKEMGVKKLIIVYNFEGISNLINKSRKAENETEKTY
                     VNYYEENIKNYVDVDFKHIDNCFNDAMHGLAKYLAKYNAYRYC"
     gene            complement(3946..4353)
                     /locus_tag="KPK72_003298"
     CDS             complement(3946..4353)
                     /locus_tag="KPK72_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2490488.1"
                     /translation="MKINFVIDGEPVGKERPRFNLATKRTYTPQKTKNYEELIKWLYQ
                     SKVKYHFTGYIKMTLRCYYSIAKSNSKKVKEKKRNNVLRPSKKPDIDNVVKIIADALN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            complement(4481..4720)
                     /locus_tag="KPK72_003299"
     CDS             complement(4481..4720)
                     /locus_tag="KPK72_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490489.1"
                     /translation="MNTLIYKYIVNEHRDEADELDRDYGEFKIFNGGKCFGGCGRNFY
                     TLAKFKKMIVTCPHCGKKYQIKLFKNGKVNMRVVE"
     gene            complement(4792..4974)
                     /locus_tag="KPK72_003300"
     CDS             complement(4792..4974)
                     /locus_tag="KPK72_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490490.1"
                     /translation="MNKRIKLKKGILHKKCDERCDNYRFIIRNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            complement(4967..5137)
                     /locus_tag="KPK72_003301"
     CDS             complement(4967..5137)
                     /locus_tag="KPK72_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490491.1"
                     /translation="MTNKEMCKSKNLDEREIYKEFSKEICASCINDKGDCESKDCDTA
                     YKIWLEKEIENE"
     gene            complement(5146..6150)
                     /locus_tag="KPK72_003302"
     CDS             complement(5146..6150)
                     /locus_tag="KPK72_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2490492.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     SSGMNDEYHLRVLDKNAEKEEANSKFVTEFLNATKVKDDKYKTKMFKSTAENWITNAL
                     GNDIKQAEDVRSILNYTLKEKHEIDINNFVDKTIKDDKLKDSFKEHMEEKGLDKGFSI
                     DKKWVEKKLKKRNIKTDNGFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVKFYEEK
                     "
     gene            complement(6147..6368)
                     /locus_tag="KPK72_003303"
     CDS             complement(6147..6368)
                     /locus_tag="KPK72_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490493.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEL"
     gene            complement(6477..6653)
                     /locus_tag="KPK72_003304"
     CDS             complement(6477..6653)
                     /locus_tag="KPK72_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490494.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            complement(6771..6944)
                     /locus_tag="KPK72_003305"
     CDS             complement(6771..6944)
                     /locus_tag="KPK72_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG2490495.1"
                     /translation="MKLKDIIKLGEKYCYCPSCGNDKIGNNEGKLIVEEHTYYRECSC
                     GFNILIDDRKDEI"
     gene            complement(6941..7081)
                     /locus_tag="KPK72_003306"
     CDS             complement(6941..7081)
                     /locus_tag="KPK72_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490496.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSRYEYEGML
                     EK"
     gene            complement(7050..7403)
                     /locus_tag="KPK72_003307"
     CDS             complement(7050..7403)
                     /locus_tag="KPK72_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490497.1"
                     /translation="MAKIWMDAGELLEKTIDIEDMFGCNLSKMRNKSKQKGLVGRINS
                     KGQGRKGKKVECTNIITGERKVFDNAVQASKCLYFTELYVGYLARTGKTTKNGWKARY
                     IKEVSDGISKCGTSN"
     gene            complement(7417..7674)
                     /locus_tag="KPK72_003308"
     CDS             complement(7417..7674)
                     /locus_tag="KPK72_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490498.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKEYVYAMFGEINNFKEIGKYEDE
                     KRAMQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPIK"
     gene            complement(7692..7913)
                     /locus_tag="KPK72_003309"
     CDS             complement(7692..7913)
                     /locus_tag="KPK72_003309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490499.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQYKEYKELWIDDIEGVEFTVE
                     KTFDDGYVCYPNEFRFNFGVISEWCVEVK"
     gene            complement(7903..8079)
                     /locus_tag="KPK72_003310"
     CDS             complement(7903..8079)
                     /locus_tag="KPK72_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490500.1"
                     /translation="MSRTAICSVCGEFLVDSYGDEFTNAKVIDLYGREFVIVICKGCL
                     EELLRKCEEQQYEI"
     gene            complement(8105..>8479)
                     /locus_tag="KPK72_003311"
     CDS             complement(8105..>8479)
                     /locus_tag="KPK72_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2490501.1"
                     /translation="ELKYLPNSGTPKILFRVAVDRKYKDREGKKKVDFIPCEQLGKHV
                     ENLVQYLTKGKQIYAFGELNIDNYKDENGCWKSITKVNVNALELLSSKEDSNNHEKQQ
                     EYIPPGLDPQGFQAIDDDDIPF"
BASE COUNT         2518 a         1640 c          751 g         3570 t
ORIGIN      
        1 gtgcttgtat atcaaacagt tcactttgat ttaactcatc tctttttaca tcttttggta
       61 tacctaattt ttttcttaca agttcagtaa agtgtttata atacatctga ggtttattgc
      121 ttccttggtt ggtagcataa taaacaaact cttgtatttc atctgtaaaa tcttttctaa
      181 cctttttgcc ttctgttctt atatccagcc attcttggtc tttttctgta gcaatatagt
      241 aaccatgtat tctaatttgt ttaagtgctt tagaaaccca ttttgtaaat agttttgctt
      301 ctgctttatt acttctaaat gaaatgttgt atatagcttc ctctgtaata aaaatttcac
      361 ccttattatt caaagcttta tcaaaatttc ggatgtgcat ttcatgcatg tctgaattct
      421 taaatttttt cttatattct ttatcaatat ttcttagtgt agagttaatt tgaattactc
      481 ctaattcctc tcctacatct ccagcgtgaa accaaacttc actaccatct tttgaccata
      541 tcatccttac atttttctct tgtaaaattt tcaacatact actacctcct aaattagtta
      601 ttaataaaag gtgttcaatt tgaataccct tattttcgcc ccctcaatat gagggattaa
      661 aattgtataa aaaaagacct agaaattaat ctaagccttc tctactgtcg tagttaccga
      721 catctgaaac ttattaattt tttgtattaa aaaagactta gaaattaatc taagcctttt
      781 ttagcaggga gatgcatata ttatgtcgca agttctaaga atcgaactta gattaaacac
      841 cagcacctgc atggtgagtg aggttaccaa gccccactca gtttttagac ttctgaatta
      901 agatacaaaa tataaaattt tgccctcaat ttctctactt ttagtgtatc cgttgattaa
      961 tatttgaaca tagttagagt tgaactaaca gcgtcctcac gccctgccta gtctgttcat
     1021 agttgtacaa ataaaattaa ttttcaccat tttacaagaa aagaacccta tttctagagt
     1081 tcttcatatt tatttaagta catttaattt taaaattcta ttagttttaa aaaatcagtt
     1141 tttttatcaa agaaatcttt atccaaacca tataaagata ttgcttcttg tttgtttaac
     1201 tttatttctt ctaacaaaga ttcttttgta aaaatgtcgt tttcaactaa aatttcaaag
     1261 gcttgtttaa ataagtatgg tttttcactc acaataacat catccagtgg ctctctttta
     1321 taatatctat tttttatcat ttgtgaatgt aagtacttaa tttgattttc agttaaaata
     1381 tcgcattctc ggcatcttct tatcatggct gaaatagaaa ctttccatct ttttttcaat
     1441 aatacaaagt tatcaattga agatgataaa acttctttat ctaaagatct taatggtaat
     1501 aaaaaagatg cagcaaacca atcagcttga tgttcaatca agtttatgtt ttcttctaaa
     1561 tcttcttcat ctatatttct atgaagaatc aaatgtccaa gttcatgggc taaatcaaat
     1621 cttgatctaa ccgcagaatc cttatcactt cctaaaaaaa catatggtat ttcactttcc
     1681 catttagaaa aagcatctat ctttttgttt ccaatttcta acttagttat gacaattcct
     1741 ttttgttgta ataaatcaat taaattttct attggagatt cccctaatct ccaatatctt
     1801 cttaactcaa ttgttatgtc ttctatttta agatcatcaa tactatcaaa tgatatatct
     1861 ccaaaatcag gtatatcaat tttaggtaaa ttaaaatatg aattaattaa cttgtatgca
     1921 ctatttatcc atgcaattct tactttgcat gcatctttta tttttttagg tatattttta
     1981 ttgcttctaa aatacactgt tccatcgctt agatccattt cttcattttc acaaaagaaa
     2041 ttaattggaa aatcaagttc atctataatt ttaattaaaa ctggtgaact aggttgaatt
     2101 tttccttttt catataatga tattgtttga cttgacactc caattaattt tgaaagttga
     2161 gctaaagata aattccttga aattctagct tgttttaatt tacttggaac tatttttctt
     2221 tcttttattg gatcaaaatt tataactttc atttttaata tctcgccttt ctaatctaaa
     2281 attctataaa actacctttt atcatcttat gaaactctaa atattatttt tatcttcatt
     2341 tccacaatac tattatctca tgctttttta atcaaaaagg ggagaaagta gggaataaag
     2401 tgggaatttc tggggaaaaa ctggggaatt ttctaatttt taaataatgg tagctcattt
     2461 tccttaattt ttggataaag catatccata actttataca ctaatctttc ccttacacat
     2521 ctacatgttt tcctatctga attcatttct aaggatatat aaaccatact atttttcatt
     2581 ctgctattat aaaacagttt aaaaaaatgt tcctctctta tatctaagca tgtaagtgca
     2641 ttttctattt tcttcttttc tatttcttta tcttttttca gttttttcaa tctagtaata
     2701 tctctttctt tttttataat ctcattctca acagttgaat taaaagcata tgttggactt
     2761 actttttcgt catatccaac agccttacat ccaaatatct cattttctct actttctata
     2821 tctaattcaa gatttttaat ttctgcactt aaaaatttat aatgatgtag tctaccttct
     2881 acttttttaa atagttcttt tttattgata ttattatcca tacttccaca ctcctgtttg
     2941 tgttataata atcttggata aaagttttat atttttgacg actggagtgt gggagcactc
     3001 cttttctctt ttagtaacct aatccttcaa aaggattagc cttcaagtca cctagtcgct
     3061 cagaacgact cacttttaaa tctaaaatat ctataccatc tttaaatctt tttatattat
     3121 tatttataag ctcatttaca tgtttaagtt ctctattatg tatcttagct atatccttta
     3181 ctgacattgc tttcttattt tctccaaatc caccctcaat attatgaaac tccattccct
     3241 caacttctaa agttccaagt actgttattt ctttatttat attttcattc ataatctatc
     3301 tctccttttt tcttttaatt aacaatatct ataagcgtta tatttagcta agtacttagc
     3361 taaaccatgc atagcatcat taaagcaatt atctatatgc ttaaagtcta catctacata
     3421 atttttaata ttttcttcat aataattaac atatgttttt tctgtttcat tttcagcttt
     3481 tctactttta tttattaagt tagaaatacc ctcaaagttg taaactatta ttagcttttt
     3541 aactcccatt tcttttattt ctctcatagc tattattgct gcttttattt ctcctgctat
     3601 atcttttaat aataatgctg ttctattttc tccattgtcc cattcttcat aaaccattcc
     3661 attaggtctg catatagcta ctccagagcc atatttattc aacttttctg aataacttcc
     3721 acttatatat gctatcatag tgtctttatc ttgctttata tcctctagcc ctaaattata
     3781 tccttctaaa tatagttttg cttgttttaa tgagtaaaat cccttatatt ctgcacatga
     3841 atacactctc acttgtttat atgcttcctc ccaattattg tatattccaa ttttatgacc
     3901 ttttctaaca gcataatatt tgctaggcat tttatttaac cctccttaaa taacatcctc
     3961 caatataacc tcaacccttg gtttatcact gtaatattta ctagctacaa cctcaacaat
     4021 ctgtgtatca tccttatatg ctatctcatt gagtgcatca gctataatct tgaccacatt
     4081 gtctatatcg ggctttttac taggtcttaa cacattattt cttttctttt ccttaacttt
     4141 tttactgtta ctttttgcta tagagtaata gcatcttaaa gtcattttta tataacctgt
     4201 aaaatgatac ttaactttag attgatatag ccattttatt agttcctcat aatttttagt
     4261 tttctgtggt gtataggtcc ttttagtagc caaattaaac ctaggtcttt ctttgccaac
     4321 tggttctcca tctattacaa aattaatttt catttttcac cttcttagct tttctctcac
     4381 atttcttgca acaataaata tccttagatt tttccttaag ataaaataat ttcccacacc
     4441 aactgcatct tcttcttttc acagaatcac ttcccttgat ttactcaact actctcatat
     4501 tcacttttcc atttttaaaa agttttattt gatatttttt tccacaatgg ggacaagtaa
     4561 ctatcatttt tttaaactta gctaatgtat agaaattcct accacatcca ccaaaacatt
     4621 tacctccatt aaatatttta aattctccat agtctctatc taactcatct gcttcatctc
     4681 tatgttcatt aactatatat ttatatatca gagtattcat taaaaacacc tacctaatct
     4741 atttcgattt cgactctagt ttcattcaca aacttacctt gactatttat tctatttact
     4801 tcttaatttc ttatagtttt cttcacatac tttatccatg ttttctttgt atttgcatcc
     4861 tatacacaca ttgcaagtta taaggttatt tctaattata aatctataat tatcacatct
     4921 ttcatcacat tttttgtgaa gtatcccttt tttcagcttg attctcttat tcattttcaa
     4981 tctccttttc taaccaaatt ttatatgctg tatcacaatc tttactttca caatctccct
     5041 tatcatttat acaactagca caaatctctt tactaaattc cttatatatt tctctttcat
     5101 caagattctt tgacttgcac atttctttat tagtcatatg ctcacctact tttcttcgta
     5161 aaattttaca ttcttaataa ttatatctat agacccattt tgattttgtc ttactgtata
     5221 tttcattggg tcctcaaaat cagtcagatt accttttata tcaaagccat tgtcagtttt
     5281 tatatttctc tttttaagct ttttctcaac ccatttttta tctatactaa atcctttatc
     5341 aagacctttt tcttccatat gttctttaaa gctatctttt aacttatcat ctttaattgt
     5401 tttatcaaca aaattattta tatcaatttc atgcttttct ttcaaagtat aatttaatat
     5461 acttcttaca tcttctgcct gttttatatc atttccaaga gcattagtta tccaattttc
     5521 ggctgtactt ttaaacatct tagtcttgta cttgtcatct ttcactttag tagcatttag
     5581 aaactctgta acaaacttag aattagcttc ttccttttct gcattcttgt ctaaaaccct
     5641 aagatggtat tcgtcattca ttccactcga tccaactagg gcagcaattt taaccgtctt
     5701 agtctcttgt atattaattt cattcttaga catttgtata ttaaatttat catctttaaa
     5761 ctctattgaa tgagtatacg aattattgta atcaagcttt aatatagcaa ctttcttttc
     5821 atctttttga gagtataagc aaattgctaa gtcgcaagat tctaatgtag cattcaattt
     5881 cataacatca aataaataag ctgcaatctc tttagagtta tttaaaaatg aactttcatc
     5941 ataaataatt tgttcgcaac acttcttaat tagattgtta ctatagtcat taaatactgc
     6001 tgttctgata tcattatctc ttgatacttt gcttattttc ttttgaaaaa ataggtccat
     6061 atcttgactg actctacctt caaaatcgtt caatattggt gtatcgctat tcttatctaa
     6121 aacatgtatt ataaatttgt gtattatcat aattccaccc cttcatcctc aaaatattca
     6181 gccattttct gacttctagt atcttttatg acttcttcaa ctttatctat tgttacaagt
     6241 aatatcttgt catctttagc taataattct gctttttgtt ttaacatttc tttacttcca
     6301 catgaataat ttatcttacg ttcatccaac aataaccctt tttgccatct aagtacatac
     6361 tttgacattt gttcacccct tattttcatt tttgagagtt acaaaacact tcaacaacaa
     6421 tttatattaa aatacatttt gcaactctct aaactgtttt aattagatat tttcacttat
     6481 atttcttcta acatttcctc gagtttattt tttaataaat catatttttc tttagtttct
     6541 aaatctaata ttctaactct tcctcgctct gctataatag ctatatttga actttcacat
     6601 atcatctgta tataatttac agaagcattt atcatttcta atctatcatt cattcttaaa
     6661 ccctctcata ttatcgcaat tttcacactc ttttaggttc aatctatact cgtaaatcct
     6721 accagcaaca aaactaatcc ctatcaaaag taaactagct aagatattca ttagatttca
     6781 tccttcctat catcaattaa tatattaaac ccacaagaac actccctata atatgtgtgt
     6841 tcttcaacta ttaatttgcc ttcattgttt cctatcttgt cattaccaca actaggacaa
     6901 tagcaatact tttctccaag ttttataata tcttttaatt tcatttttct aacatcccct
     6961 catactcata tctacttaat atttttatag ctatatcaat agctttatta acagaacact
     7021 ttttcttatt taatatcttt tcagctaatt taattacttg ttccacattt gctaatacca
     7081 tctgacacct ctttaatata tctagctttc caaccatttt tagtagtctt gcctgttctt
     7141 gctagatacc caacataaag ttctgtaaaa tataaacatt tacttgcttg aacagcatta
     7201 tcaaacacct tacgctctcc agtaatgatg ttagtacact ctaccttctt accttttctt
     7261 ccttgtcctt tagaatttat tctaccaact aaaccttttt gtttactttt atttctcatt
     7321 ttacttagat tacatccaaa catatcttct atatcaatag ttttttctaa caattctcct
     7381 gcgtccatcc aaattttagc catgttcttt ccccctttat ttaattggca tttgaaatat
     7441 tctatttcta taacttctaa ccttataact gtctatagaa tccgttctag tcccaccttc
     7501 aataaatctt tgtatattat ctaatacctg catagctctt ttttcatcct catattttcc
     7561 tatttcctta aagttgttta tttctccaaa catggcatat acatattctt tatcaacatt
     7621 tatccaatca gctttcacta aatctgtttt atcttgactt ctaattatta tcatctctaa
     7681 taccccctac cttatttaac ttcaacacac cattccgaaa ttacaccaaa attaaaccga
     7741 aattcatttg gatagcaaac atagccatca tcaaaagttt tttcaacagt aaattcaaca
     7801 ccttcaatat catcaatcca aagttcttta tattctttat attgttctac accattaatt
     7861 tccataaatt tctctttatc aaacttataa gttttaccta tctcaaatct catattgttg
     7921 ttcctcacat tttcttaata gttcttctaa gcaacccttg catataacaa ttacaaattc
     7981 tctaccatac aaatctataa ctttagcatt tgtaaattca tcaccataac tgtcaactaa
     8041 aaactcccca caaacactac atatagctgt tctactcatt tttatccctc catttttaac
     8101 ttaattaaaa aggtatatcg tcatcatcta ttgcttgaaa accttgtggg tctaatcctg
     8161 gtggtatata ttcctgttgt ttttcatgat tattactatc ctctttactt gaaagcaatt
     8221 ctaaagcatt tacattaacc ttagtaatag atttccaaca accattttca tctttgtaat
     8281 tatctatatt taattctcca aaagcatata tctgtttacc ttttgtaaga tactgtacaa
     8341 gattttctac atgttttcct aactgttcac aaggtataaa atcaactttc tttttgcctt
     8401 ctctatcttt gtattttcta tctactgcta ccctaaataa aattttaggt gtacccgaat
     8461 ttggaagata tttcagctc
//
LOCUS       DAEMHZ010000155         8445 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000155 DAEMHZ010000000
VERSION     DAEMHZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.156"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            219..959
                     /locus_tag="KPK72_003312"
     CDS             219..959
                     /locus_tag="KPK72_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG2490502.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGGFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            971..2749
                     /locus_tag="KPK72_003313"
     CDS             971..2749
                     /locus_tag="KPK72_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2490503.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            2916..3833
                     /gene="pfkB"
                     /locus_tag="KPK72_003314"
     CDS             2916..3833
                     /gene="pfkB"
                     /locus_tag="KPK72_003314"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2490504.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEYEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            3870..5789
                     /locus_tag="KPK72_003315"
     CDS             3870..5789
                     /locus_tag="KPK72_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2490505.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            6384..7910
                     /locus_tag="KPK72_003316"
     CDS             6384..7910
                     /locus_tag="KPK72_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2490506.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            7938..>8445
                     /locus_tag="KPK72_003317"
     CDS             7938..>8445
                     /locus_tag="KPK72_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490507.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDAL"
BASE COUNT         3441 a          754 c         1535 g         2715 t
ORIGIN      
        1 aaatatacta ctgtatattg attataaact ataaagctta tgtaaagtaa ataaattatg
       61 taaaatttag gttaattttg tataaagaga tacttttata ttttacaatt aataaccaaa
      121 tactaaaaaa tattttaaat aaagaactta aaattgtgat aaaattaatt tatagatatt
      181 aaaaaaagta taatatatgg tttttaagag gtgctaagat gaaaaatttt agagatttag
      241 gtgggaacaa aactgaggat ggcagaactg taaagaaggg attattttat aggtctgcaa
      301 aattatcaaa tctaagtgaa aatgatataa agattttaaa agagttaaat attaaatata
      361 tatttgatta tagaagtgat gaagaagctc gtaaacatcc atcaacaata atatcaaata
      421 taaaaaatat aagaatacca gcaatgagag agctagaaga atcaggagga ggttttggtt
      481 ctattgaaga tatgattgat ggattgtttg aaaaagatgg tgcattcaat atgttaaata
      541 atagttacta taatttgcct ataaataatc cgtcatataa aaaattagta gaacttataa
      601 gagactattc taatttacca atattaaatc attgtacagc tggtaaggat agaactggtg
      661 tggggagtgc tataattctt atgattttag gggtatcgag agagaatata atgaaagatt
      721 atctaaaaag taatgatttt gcagataaag agatagaaag atttatagaa tataagccta
      781 aatttaagga cattccaaaa gaaaatttga aatatatatt tggtgtaaat gaagagtata
      841 tgaaaactgc atttagaaga attgatgaag aatacattag tgtagaagca tatttatatg
      901 gagaatttaa tttaaataaa gaagaaataa gaaaattaag aaatcaatat ctagagtaaa
      961 aggtgagttt atgagtttaa atataacagc aagacagata aatataataa aaatattgtt
     1021 aaattcaaaa gatacaatta gtggtattgc cttaagtcaa gagataggat gttcttcaaa
     1081 aactatacaa aatgaaataa aggatattaa taaacaactt agaaatggta aaattttgtc
     1141 aataagagga gttggttata aactagaagg aagttttgat gaggtaaatc taaatagcaa
     1201 tctctatgat gatgtagata gagtagaata tataatcaga aaaatattaa ccttaagtaa
     1261 tgaagaaaaa aatactataa aacttgaaga tttagccgat tctatgtatg taagtgtttc
     1321 aacagtaaaa aatgatttaa aagaagtaaa aagaatattg gaaaagtatt caatatcagt
     1381 taaatctaag cataaacaag gtatatgtgt tttggaaagt gaagataaga tattaaattg
     1441 tatagttgac ttaagcaata agagagataa tcaattgagt ttaaatgatt ttttaaatga
     1501 tgatataaag gaaagaaaac tattaataaa aaaattatta ttagacatat taaatgaaaa
     1561 tggattagta ttaactgata tagaatttaa gagtgtgtta aataatatat tagttatgtt
     1621 aagcagacat aagtataatg aaaaagaatt tatagagaat tatatagaaa attataggag
     1681 caaaagaagc tcaattttag aaaatgaaca aaacaaagaa attataataa attcaataaa
     1741 aacattttgt aaaaatttaa aattagctac atcaatagat ataagtaatg ataaggtatt
     1801 tgaagagttt ttatataagc atattagtag tttatgtaaa aaaataaagt taggtataaa
     1861 tcaatcacct ataatggctc aagatataaa aataaaatat ccatttgctt ttgaactagc
     1921 taatatagct aaaaagacaa tagaaaaaga tttgaagata gaaattaatg aagatgaagt
     1981 tgcaaacata gctctacata taggaggagc agttgaaaga gcatcttata ataataaaga
     2041 taaggttttc aaaactataa tagtttgtac ttcaggcata ggcacatcaa tgttaataaa
     2101 agctaaatta gagaatatat ttaaggaaaa attagaaata ctaaagatta ttccttcata
     2161 cttagttgaa tatataaaag ttatagatgt tgattttgta atatctactg ttcctattga
     2221 agtaggagat gtgccagtaa taaatatatc tccaatttta agtgagaaag aaattagatt
     2281 aattgaaaaa tatatagaaa caggaaatat atacctagat ttagatataa aaagcctgtt
     2341 tgatagtgaa ttatttttta ctgatttggt gtttaaaagg aaagaagaag ttatagattt
     2401 tatggcaagt aaacttgtac aaaagggcta tatagatgag gatatgaaaa aatcttactt
     2461 tgagagagaa aaaatagcaa caacagaaat agggaatatg gtaactatac cacatggagc
     2521 tattggaaaa atttttgata ataaaatagc aattggtata ttaaagaagc ctatttcttg
     2581 ggaaatatca gatgttcgtt taataataat gttagccctt gataaagata aaatattaga
     2641 ttatgaagta atattttcaa aaatatataa aagagtagat tctatagcaa aagttataag
     2701 tatatgtgaa aatagtagtt ttgaaaaatt tgtaaattta tttaattaga tgggtagtat
     2761 tgtatataca cctgtaaatt tggataatat aaaactaaat aagataaaaa cgataaattt
     2821 ctcttttaat taagagaaat tttttgactt tcaaggatat taatataaag ttaagatata
     2881 aacataaagc aaataaataa aaaagaggtg gcaatatgat ttatacagtg acattaaatc
     2941 catcaataga ttatatagtt aagctagatg agctaaagac aggaagtaca aatcgtgtaa
     3001 aagaagaata cgtttatcca ggaggaaaag gtataaatgt atctcgcata cttaaggaac
     3061 ttggaaatga taatacatct ttaggtttta taagtggttt tactggagaa tatataataa
     3121 gaacattgga agaaaaagaa ttgaagacag attttataaa gataaaaagt ggtttttcaa
     3181 gaataaatgt aaaaataaag gcgttaaagg aaactgagat taatggacaa ggtccaaata
     3241 tagatgatga agacattgat actttatata aaaaacttga taagctaaat caagatgata
     3301 ttttaatatt ggcaggaagt ataccatcta ctttagatga aaaactatac gaaaatataa
     3361 tggctagatt agaaaaaaag aatataaaag ttgtagtaga tgcaactaaa aatcttcttc
     3421 ttaatgtttt aaaatataaa ccttttctaa ttaagcctaa taacgacgaa ttagaagaac
     3481 tatttggtgt taaattaaat tcaattgaag acatggtaaa atatgctaga aggttaaagg
     3541 aaatgggagc tataaatgtc ttggtatcaa tgggaaaaga tggagcattg ttgattacag
     3601 aggaatatga agtgcttata agtgatgttc caaaaggaaa agtaaaaaat tctgtaggag
     3661 caggtgattc aatggtagca ggatttatat ctgggtattt gaatactggg aaatatgatt
     3721 atgctcttaa attgggagca gcttcaggta gtgcaactgc tttttcatat gacttagcca
     3781 aaagagaata cattgataaa ttggtaaatg agatatctgt aaagcagttt taaaatatta
     3841 aaaaatatat agaaaggtaa ttaatattta tgaaaattat agatttatta gatgaaaagt
     3901 caattaagtt aaatttaaat tcaaaaacca aatcagaagc aatagaagaa ttagtagatt
     3961 tggtcgcaaa tagtggaaat ttaaatgata aagaaaacta caaaaaagca attttggctc
     4021 gtgaagaaat gagtacaaca ggaattggag aaggtgtagc tatacctcat gctaaaaatt
     4081 cttcagttac taaagcttgt atagcagcag cagtgtcaaa agaaggtata gattatgaat
     4141 cgtttgacgg aagtttatca aatctatttt ttatgatagc tgcaccagac ggagctaaca
     4201 atactcatct tgaagtatta tcaagattat ctacaatact tatggatgaa gactttagaa
     4261 ataaacttat aaattcaagc tctgaaaaag aatttaagga aataatagat aaaaaggaaa
     4321 gagaaaaatt cagtgaagaa tatcaagatg agaaagttac tgaaaaaaat ataatagata
     4381 ctaaagagaa tgatgctaat aagtatccta aggttttagc agtaacagca tgtccaactg
     4441 gaatagcaca tacatttatg gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg
     4501 tatcaataaa agtagaaact aatggttcag caggtgttaa aaataagctt acaaaagatg
     4561 aaatagaaaa tgcaacatgt ataatagtgg cagcagataa aaatgttgag atggctagat
     4621 ttaatggcaa aaaagtcata aaaactaagg tggctgatgg tatacacaaa gcggaagaac
     4681 ttatagacaa ggctgtaaat ggagatgctc caatttacca tggaggagat ggaagtcaca
     4741 acgaatcaaa tgaagaaagt gaaagtggat ttagaaaagt atacaaacac ttaatgaatg
     4801 gtgtatctaa tatgttacca tttgttattg gtggagggat acttatagcg atagcctttt
     4861 tacttgatga ttatactatt aacccttcaa actttggttc aaatacacct atagcagcat
     4921 tttttaaagg aataggagat aaagcttttg gatttatgtt acctgtactt gctggatata
     4981 tagcatactc tatatctgat agaccagcgt ttgttgtagg atttgtagga ggtgctctag
     5041 caggtgatgg tggttcaggc tttttaggtg cactacttgc aggttttata gcaggttatt
     5101 tagtagaagg gcttaagaaa atatttagtg tactaccagc ttcattggaa ggaataaaac
     5161 cagtattgtt atacccttta cttggaacac ttttgatggg aattataatg acatttttag
     5221 taatacctcc agttacagca attaataatg caatggttgg atttttaaat ggtttaggtg
     5281 gaacttcaaa aatattttta ggattagtat taggtggaat gatggctgtg gatatgggtg
     5341 gacctgtaaa taaagcagca tatgtatttg gagttgcatc acttgaatca gggcagtttg
     5401 aaataatggc agcagtaatg gctggtggta tggttccgcc acttgctata gctcttgcaa
     5461 ctacattttt taagaataga tttactaaag aagaaagaga ttctggtaaa gttaattatg
     5521 taatgggatt atcatttgtt actgaaggag cgataccttt tgcagctgga gacccacttc
     5581 atgttatacc agcttgtgtt ggaggttcag cagtggcagg agcacttagt atgttgttca
     5641 acgcagctct tcgtgcacct catggaggag tatttgttat accagtggtt acccatccat
     5701 ttgcatatat attagctata gctgtaggtg ctttggttgg aatgatgctt ttagcattac
     5761 ttaaaaaacc attaaatcaa gaagtatagc aattaaagga tattcaagaa cttaaaatat
     5821 gatgttttat aaaaattaat atattgctga attattttaa aataaggatt ttatgaggct
     5881 gttatataac gatttgtaat tgttatcttg acagtctttt atttttatat aagtaaaata
     5941 agtgtgtttt gctttatttg tatttaaatt gtttttaagt tagaatataa agaaaattat
     6001 atattatttt aatattcact cgaactagaa aataatattt gttgtatttt ttgtatataa
     6061 aatatattgt aatattaaaa aaataatagt aaactgattg cataaaaatg atttttatat
     6121 aaaatttaag cagaagaata aatttctaac gaaattggat atttcttctg taaatacata
     6181 ttcaaaaaaa gaattataag agtttagtca gcagtcgtgt aagtaataag gactttataa
     6241 atatatagaa atataatgat attaatataa ccaagcgatt tttaatttag ctttttaata
     6301 agcttatttt ttatactatt tataataaat aaactccatg atgggaaaac tattactatt
     6361 taatatgtaa aggagtattt taaatgaatg aatcatattg gtttttaaac tcaagtccaa
     6421 aagaatataa caaattaggt gaaaatataa aaacagattg tcttatagta ggtggtggaa
     6481 taactggatt gacaactgca tatttattag caaaagaagg taaaaaagta gtgcttgttg
     6541 aggctgataa aataggatat ggaacttctg gaaggaatac tggtaaggta acttgtcagc
     6601 atgatatatt ttactctaag atagagaaaa aatatggatt agataaagca aaatcatatt
     6661 ataatgcaaa taatgaagct ttaaatttag ttgaacaaat aatagaagaa aacaatataa
     6721 aatgtgattt taaaagggaa acttcttttg tatttacaga aaaagaggat actataaaga
     6781 atattaaaga tgaatatcgt acatgtaaaa aaataggaat aaattgtgag tatcatgaaa
     6841 ctatagaaaa tattccatta gatataaagg gggctattag ttttactaat caaggtcaat
     6901 ttaacccaaa gaagtatatt gatggattgg caaaagcagc agtaaattta gggctaaaaa
     6961 tatatgaaaa tacaccagta gtagaccttg aaaaaggaaa aatatgtagg gttaaaacaa
     7021 gagaggataa tataatagaa gctgagaatg ttataatttc atctcatagt ccatggtatg
     7081 atggattgaa tttttatttt gcaaaagaat atgcagagcg tgcttattta atggttgcag
     7141 ttttggaaaa taaattagca gatggtatgt ttataagtat agatgaccca tcaataactt
     7201 ttagacaata taatgatgga agtgaaaatt tactgatttt tggaggagga gaccataaag
     7261 ttggtcaggg aggaactgag aaagaaatat ttgatgattt agagcattat ggtaaagaag
     7321 tctttaaggt aaaagatttt aaggggaaat ggtctgctca agacaatatg agttttgaca
     7381 atgttcctta tattggttat atcaacaaaa gagaagacaa tatttatgta gctacagggt
     7441 tctctaaatg gggaattact aatggaactg ctgctggaat aataataaaa gatttgataa
     7501 taaataataa ttctgattat aaagatactt ttaatccatc cagattaggt agctactttt
     7561 caaaagattt tataaaggaa aatgcaaacg tagcaataaa ttatgtaagt ggaaaattaa
     7621 aaataggtag tggagatatg cctaaaaata atggagaagg aaagattgtt aatattgatg
     7681 gaaaaagata tggtgtatat aaggatgata atggagattt ttatattgta gacacgacat
     7741 gtactcattt aggatgtgaa cttaatttta acagtgaaga aaaaacatgg gactgtccat
     7801 gtcatggttc tagatttgac tataagggaa atatacttga aggaccagct cttaagccat
     7861 taaaacttta tggacatggt gataatgatg ttaatcctaa attattataa aattattttt
     7921 aggaaggtaa agtgtatatg aataaacttt taaagccaat aactatacta ttgtatatag
     7981 tttatatttt atctgccgta ttttttgtag caaacaaaga atatggaagt ctaggtctag
     8041 tagcaattag tttagttggt actttcattt tgagttttat aaataagcaa actgaaagat
     8101 tattgagtaa agaattatat ataatgcttg ctttatttat aatgttttca tcattgttag
     8161 gtacttgttt taatttttat gatataaatt attatgatga ttttttgcat ttatggtcag
     8221 gtataatatc ttgtattgtg gctttttcta taattaaata cttttataca caaagagaga
     8281 taagtaaaat gagtaaagtg tttttagtta tatttttatt tatgtttagt atgggagtag
     8341 cgagtttatg ggaaataggt gaatttttaa tggatacttt tgttgggact actacacagg
     8401 ccggagggtt agaagatact atgattgata tggtagatgc tttaa
//
LOCUS       DAEMHZ010000156         8433 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000156 DAEMHZ010000000
VERSION     DAEMHZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.157"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..1713
                     /locus_tag="KPK72_003318"
     CDS             19..1713
                     /locus_tag="KPK72_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490508.1"
                     /translation="MTTEWNFDYTGAEQSVTLKPGKYKLECWGACGGGWFSEWTKGAK
                     GGYSKGELTLKKETILYVYAGETGCKPFTNGINNWTGFNGGGRGANASVGPKFVLCGG
                     GATDIRLIRGSWSNEQGLLSRILVAGGAGAISSSDYGAGNGGGMEGSKGFGYSNAFVT
                     GGTQYQGGIGLEDKYNGLFGRANSTGTGQGGGGGWFGGAGGLTYNAAGGSGYALTKDS
                     YKPPGYIPTSEYYLENVVMTTGGNTTRADGYAKITLLQALPFLTLSSYNSTTATFKAD
                     HTDPTLLTKIEWFIDDVLKETITTELTIEKTINYTLEDNALHTIKIVVTDSSNAKAER
                     IFTVSKGIAPLPTGSSSEEVTNKWREIGTAFKTGKTSIINTLALKNIEASLNNTLVEL
                     SEKIKTSFDSSNASVQELINKLTQANNTISQLNSKYKYASGTIDVVKNSSLMANLYGE
                     SFGRQPGTWLKIDNLGFIPNIFVAECQYVTSNNYIFKHIVIATYNIKWFWDKKDFSAR
                     IEFTKEKNSNQDFSGSGIIYSNNERDVYINNKGINVPASSPNVSSYLHSWHAIKFI"
     gene            1727..2020
                     /locus_tag="KPK72_003319"
     CDS             1727..2020
                     /locus_tag="KPK72_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490509.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     VDYTKSRIVGINIETKEPILEEIPVFVSEEEKRIQELENQLLIAENEKVGGIL"
     gene            2020..2202
                     /locus_tag="KPK72_003320"
     CDS             2020..2202
                     /locus_tag="KPK72_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490510.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRPFELDDVTNTEYRKAVED
                     YIIKESGVVEGTEPTA"
     gene            2238..2468
                     /locus_tag="KPK72_003321"
     CDS             2238..2468
                     /locus_tag="KPK72_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG2490511.1"
                     /translation="MNEEVIKEKIKRNEIRINRHSDEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLIGFFIFAVQRGIF"
     gene            2484..2741
                     /locus_tag="KPK72_003322"
     CDS             2484..2741
                     /locus_tag="KPK72_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2490512.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPVILLAL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGINQTCKQLKDGEK"
     gene            2741..3556
                     /locus_tag="KPK72_003323"
     CDS             2741..3556
                     /locus_tag="KPK72_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2490513.1"
                     /translation="MKIGINCGHTKTGAGSGAIGRINESTETRNVGYKVIDKLKKLGN
                     NVVDCTIDKASTQSECLSKITAQANRQDLDWFISIHFNAGGGKGCEVYTYKGKQYQDA
                     VDVCKNISKLGFTNRGVKDGSGLYVVKKTKAKSMLIEVCFVDTEDANKYLSLGADKLA
                     TAIVEAITKHISSAEENNYNRYKHTIVYSGDDKVSADILGLYYKREKESYLVTDIKDY
                     KAHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKAIEFVKEKL"
     gene            complement(3638..3820)
                     /locus_tag="KPK72_003324"
     CDS             complement(3638..3820)
                     /locus_tag="KPK72_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490514.1"
                     /translation="MSKDMIDECVHIMTAYITSLKEYYSFIETQIDDFIKKYGEDIVE
                     SCLHRIMILLCECGLV"
     gene            complement(4696..4884)
                     /locus_tag="KPK72_003325"
     CDS             complement(4696..4884)
                     /locus_tag="KPK72_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490515.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            5141..5317
                     /locus_tag="KPK72_003326"
     CDS             5141..5317
                     /locus_tag="KPK72_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490516.1"
                     /translation="MKTLMFILVIAYYITIIIKSITTIVKSITRLLDSISKLKESLHK
                     LRNRPDCHQDDFNE"
     gene            5836..6213
                     /locus_tag="KPK72_003327"
     CDS             5836..6213
                     /locus_tag="KPK72_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2490517.1"
                     /translation="MKEEVLIEKLLRAELIVMEYLWKKDYLMPKKEIVKEMRQVCGWH
                     KSTIKILLKRLVEKGYLARDIIKFQSHYKIIIDNEEYYAFKKKVLKSSKSRKIMHSLT
                     TTHKNVSKEKLDSLEEYYKNLKE"
     gene            6318..6704
                     /locus_tag="KPK72_003328"
     CDS             6318..6704
                     /locus_tag="KPK72_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2490518.1"
                     /translation="MKISKLPEAELKVMRYIWESDKILTSREITEAMEQKYEWKDTTT
                     FTVLKRLQRREFLETEKIDKRTHYNVLVKEKQYQRFETREFIKNIHKNSIKSLISALH
                     KDDEKVDEKKLDELEEYFNNLEENEK"
     gene            complement(6895..7740)
                     /locus_tag="KPK72_003329"
                     /pseudo
     CDS             complement(6895..7740)
                     /locus_tag="KPK72_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
     gene            complement(7931..8344)
                     /locus_tag="KPK72_003330"
     CDS             complement(7931..8344)
                     /locus_tag="KPK72_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490519.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         3359 a         1006 c         1293 g         2775 t
ORIGIN      
        1 tagaaaggag tgtgaaaaat gacaaccgaa tggaattttg attatacagg tgcagaacaa
       61 agcgttacat tgaaacctgg taaatataaa ttagagtgct ggggtgcttg tggaggaggt
      121 tggtttagcg aatggactaa aggtgctaaa ggtggttatt ctaaaggaga acttacatta
      181 aaaaaagaaa ctatattata tgtttacgct ggagaaactg gatgcaaacc gtttacaaat
      241 ggtattaata actggactgg atttaatgga gggggaagag gtgctaatgc aagtgtaggc
      301 cctaaatttg tattatgtgg tggaggtgct actgatataa gacttattag aggaagttgg
      361 agtaatgaac aaggattact atcacgtata cttgttgcag gtggtgcagg tgcaattagc
      421 agtagtgact acggcgcagg aaacggtggt ggcatggaag gttcaaaagg atttggatat
      481 tctaatgctt ttgttactgg aggtacacag tatcaaggag gaataggact tgaagataag
      541 tataatggtt tgtttggtag agcaaattct acaggaacag ggcaaggtgg aggaggtggg
      601 tggtttggcg gtgcgggcgg tcttacttat aatgctgcag gtggaagtgg ttacgcacta
      661 actaaagata gttataagcc acctggatat ataccaacat ctgaatatta tcttgaaaat
      721 gtagtcatga ctactggagg taatactact agagcagatg gttatgctaa aataacatta
      781 ctacaagcat taccgttttt aacattatcc tcttataact caactacagc aacatttaaa
      841 gcagaccaca cagaccctac attattaact aaaatagagt ggtttattga tgatgtatta
      901 aaggaaacta taacaaccga gttaacaata gaaaaaacaa ttaactatac attagaagat
      961 aatgcactac acacaattaa aatagttgtt acagacagtt ctaatgctaa agcagagaga
     1021 atttttacag ttagtaaagg aattgcaccg cttccaactg gctcatctag cgaagaagtt
     1081 acaaataagt ggagagagat tggaactgca tttaaaactg gtaaaacaag tattataaat
     1141 actttagcat taaagaatat agaagcaagt ttaaataata cacttgtaga attatcagag
     1201 aaaattaaaa cttcttttga tagttcaaat gctagcgtgc aggagttaat aaataaatta
     1261 acacaagcta ataatactat aagtcagtta aactctaaat ataaatatgc cagtggtact
     1321 attgatgttg ttaaaaatag ttctttaatg gctaatttat atggagagtc ctttggtaga
     1381 caacctggta cttggcttaa gattgataat ttaggtttta ttcctaatat ttttgttgct
     1441 gaatgtcaat atgttacttc taataattat atttttaaac atattgttat tgctacttat
     1501 aatataaagt ggttttggga taaaaaagat ttttcggcta gaattgaatt tactaaagaa
     1561 aaaaattcta atcaagattt tagtggaagt ggaattattt attcaaataa tgagcgtgat
     1621 gtatatataa ataataaagg cattaatgtt cctgcaagta gtcctaatgt ttctagttac
     1681 ctacattctt ggcatgctat aaaatttata taaatgaggt gataaaatga atagagcaaa
     1741 tagaattata tgtgaccaaa ccgggaaaat attactccaa actggagaag caacagggga
     1801 tatattagaa catgatgaaa taacggaatt acattttgtt gatattccgt atggaagtgt
     1861 agattatact aaaagcagaa ttgtaggcat aaatatagaa acaaaagaac ctatcttaga
     1921 agaaatacca gtatttgttt cagaagaaga aaagagaata caagagttag aaaatcaatt
     1981 actaattgca gaaaatgaaa aagtaggagg aattttataa tgaatataaa taatgttgta
     2041 gtaagaatat tggcagagag aatattaaac aaaggattga accctttaaa aaacagacct
     2101 tttgagttag atgatgtaac taacacagaa tacagaaaag ccgtagagga ttatataatt
     2161 aaagaaagtg gagtagtaga aggaacagaa cctacggcat aaggttcttt tttatttcaa
     2221 ttaattagga ggcttacatg aatgaagaag ttataaaaga aaaaataaaa cgaaatgaaa
     2281 taagaataaa cagacacagt gatgaaatag acgaattaaa gatagcaaat atagagtcta
     2341 aagcagagtt gaaagcattg tgtgagaacc taaactcact tacaagtatg ttaaaatggc
     2401 taattggaac aatgattaca acactgatag gattctttat atttgcagtt caaagaggaa
     2461 tattttaatt aggaggttaa gatatggata atttaataag ttttatacca gagcaactgc
     2521 ttttattagt agttgctctt aatgtattag gatttggatt taagaaatat aaacaactag
     2581 ataataaata catcccagtt atactgctag cacttggtat agtattttca atatggatgc
     2641 taggatttaa cccaagttca attttgcaag gaattttatg ttggggagtt gcaataggta
     2701 taaatcaaac ttgcaaacag ttgaaggatg gtgaaaaata atgaaaatag gtataaattg
     2761 tggacataca aaaacaggag caggaagtgg agctataggt agaataaatg agtcaacaga
     2821 aactaggaat gtaggatata aagtaataga taaattaaaa aaattaggta ataatgtagt
     2881 cgattgtact atagataaag catctactca atcagaatgt ttatctaaga taacagcaca
     2941 agctaataga caagatttag actggttcat atcaatacac ttcaatgcag gcggtggcaa
     3001 aggatgcgaa gtttacacat ataaaggtaa gcagtatcaa gatgctgtag acgtttgtaa
     3061 aaatatatct aagctgggat tcacaaatag aggtgtaaaa gatggaagtg gattatatgt
     3121 agtaaagaaa acaaaagcta aaagtatgct aatagaagta tgttttgtag acaccgaaga
     3181 tgcaaataag tatttgagtt taggagctga taaattagct actgcaatag tagaagctat
     3241 aactaaacat ataagttcag cagaagaaaa caattataat agatataaac atacaatagt
     3301 gtacagtggt gatgataaag tatcggcaga cattttagga ttatattata agagagaaaa
     3361 agaaagttat ttagttacag atataaaaga ctataaagca catagaacac aaaatttgta
     3421 tgtaattggt ggagtaactt gtaataaaat gaaggaaatg agtaagacta caggagaaaa
     3481 atttactcaa ctatatggta atgatgtatg gtcaacaatg gataaagcta tagaatttgt
     3541 aaaagaaaaa ttataaaaca tataatttaa taaaatagat ataaagataa gaagagttta
     3601 tataaggttc ttcttatctt ttttaataaa ttgaatttta tactaaacca cattcacata
     3661 ataaaatcat tattctatga aggcaagatt ctacaatatc ttctccatat tttttaataa
     3721 aatcatcaat ttgagtttct ataaatgaat aatattcttt taaactagta atatatgcag
     3781 tcattatatg aacacattca tcaatcatat ctttagacac aatcaatccc cctaaaaaac
     3841 gaaaaataaa gaatattcag taaattatgc atattacatt atctaaaatc ttatctaaac
     3901 aatacttcaa aatttaatat tttacaagta gttatgcaaa actaggcatt ataattgtaa
     3961 aactggttat atttattaat tgcataacta aagaaaagta cataataata agaatattga
     4021 taggtttgat accttttaca aggcattgga ttttatagat aaataggttc ataatctaaa
     4081 attaagatgt ttatttacac tttacttaca tttttaatat aaagagaact taaatatatg
     4141 atataactaa ggtagttgta cttatgttaa tttgatttta aatttacaag attatttaaa
     4201 tggagaatat aattttatag aaatattgga tatatatcaa ctcgaaaata aaattatgct
     4261 cagattggta aaatatgagc ttacaaaata caataaaata atacaacatt agataaaaag
     4321 atggtaggtt aattctagca tctttctaat ttccaaacat aattttttat tagttaaaaa
     4381 actataatac atattgtaat aataaaacga acgaaaatta tcatactaaa acataagaca
     4441 tgctataatt atattgcata tttcataaat atacatttaa agcactcttt tatatgagtg
     4501 cttatttttt tgaaattcat caacatataa actatcaaga acattactca acacacctta
     4561 aaattgattt aaataggtct ttttcataaa gtaaattata tgatataata aaaaagtaaa
     4621 tatgtaaccc caatgcatct ttactgagtc aaacattatt atatagagca ttcttcatta
     4681 tggagagtgc ttttttcatt tctttgaata accatgttga ttatttataa tattttcatt
     4741 taatttatct ttatctatca aatttaatgc acaattaata caaatgttag ttttcatatg
     4801 acttttctta tgaaaagtaa tatacttatt atcatcttta tttattcctt tattacagta
     4861 atcacataat atagtcttag tcatattttt attcctttca ttttatattt tccataatta
     4921 agtttaacat atttagtata tacctatgta tatatatatt taaaacaatg ttttttattg
     4981 caatatagaa tatattgtat aaataaaaaa tatgaagaat atatatttga taattgtaaa
     5041 attatgttat aattaaaatg caagaatata tcttgtaaga aactacaatc taagggcgaa
     5101 agttttcaga aatcaccttc gtatgaaagg gggtgagtat atgaaaacac ttatgtttat
     5161 acttgttata gcgtattata ttactattat aataaaatct attacaacaa tagtgaagtc
     5221 tataacaaga ttattagatt ctataagtaa attaaaagaa tctttacata aactaagaaa
     5281 tcgtcctgac tgccatcaag acgatttcaa tgaataaatc attacttaaa ttattttgaa
     5341 tttgaaactt cgctctaggc taatagattg tagtttcttt ttttattttt gatatgattt
     5401 tacatcttta ttatatcgca ttttaacaaa aaataaaact gtgaatacta gaaatatatt
     5461 ttattttata tctaagtgta tattattacc ccgcctaaaa aactttcaca aaatcaaaag
     5521 agtatatgtt tctgataact tatttatata acttataact catcttacga atcttattta
     5581 ataaatattt atgccattag atttctctaa ttttgccaat ttacttctat tctacaatta
     5641 aaacaataca atacaactaa ttattattca acatatgtgt ttgaaagttg aataatttta
     5701 tagatatgaa agttattttt cttctaagaa aacaaactaa aagagtagaa tatttggtaa
     5761 aacttacctc tagtttttgt cttaaaaagc tcctattatt aatttaagtt agacgaatta
     5821 aggaagtgac ataaattgaa agaagaagta ctaatagaaa aattattaag agcagaatta
     5881 atagtaatgg agtatttatg gaaaaaagat tatctaatgc ctaaaaaaga aattgtaaaa
     5941 gaaatgagac aggtttgtgg atggcataaa agtacaataa aaatactact aaaaaggcta
     6001 gttgaaaaag gttatttggc tagagatatt ataaaatttc aatctcatta taaaataata
     6061 atagataatg aagaatatta tgcttttaag aaaaaggtac taaaatctag taaaagtaga
     6121 aaaataatgc attcacttac cactacgcat aaaaatgtaa gtaaagagaa gttagactca
     6181 ttagaagagt actataaaaa cttaaaagaa tgatttggta aaaattggat ggaattattt
     6241 atcttaattc tcttgtttgt aaaactttat tgatatagaa tttaacatat acataaatcg
     6301 gtaaaggggt gtagactatg aaaattagta aattaccaga agcggaatta aaagttatga
     6361 gatatatttg ggaatcagac aagatattaa catcaagaga aatcactgag gctatggagc
     6421 aaaaatatga atggaaagat acaactacat ttacagtatt aaaaagattg caaagaagag
     6481 agtttttaga aacagagaaa atagataagc gtacacacta taatgtatta gtaaaagaaa
     6541 aacaatatca gagatttgaa acaagagaat ttataaaaaa tatacataaa aattcaatta
     6601 aaagtttgat ttcagcatta cataaagatg atgagaaagt agatgaaaaa aaattagatg
     6661 aactagaaga gtatttcaat aatttagaag aaaacgagaa ataaaatttt acaaagaaaa
     6721 aatgggtggc aaattgtttt tgccacccat ttgccaccaa actggtttaa taaaataaaa
     6781 tacaaataaa gaagcataaa gaaaataaaa taaagaaatc gttttagatg aattgaaaat
     6841 agatgtattc ataaaaacat aaataaatat aaataagcat aaagaaaata tgagaatttc
     6901 aagagtaaat tcagctcttt caggtaccac atttgatgaa gatggataga aattcatctt
     6961 tcctacagta gcaaccaaat cagcagtcgt tttagctact ttttcgatag ctaatacaca
     7021 ttcacaggca caacatccag catcaagtct catattcatt ggaagtgtac cagcatgtcc
     7081 agacttacca tttactgata tcttaaaact tttaaatcct gtaatacttg atacaattcc
     7141 agcaggaaga gattcatttt ctaaaacagg accctgctca atatgaaatt ctaaatatgc
     7201 atctacatct tttctaggat gcaaatttgt taagtttttt gtattaaatc caaactttgt
     7261 tacagcttct tcaagtgtaa ttccattttc atcaacagat ttaagaagtt cctctttaaa
     7321 agtaccagca aaaactttac ttcccattaa agaagtttca aatctagttc cttcctcttc
     7381 agcgaaagca attagcttaa tattgaaagg ataagaccta ttattgtgtt ttgcttcttc
     7441 aagaccaact agaggcacta ttatacctaa cataccatca aatatacctc cacactttac
     7501 actatcttga tgagatgcaa ctactaatgt gggagcattt ggaaagcttg atttatatgt
     7561 acctataacg tttccaatgt catcaatttc aatatcaaga tttaaacctt ccatccatga
     7621 actaattaaa tcaatagatt ttttatgttc ttgagtcaag ataagtcttg taagaccatt
     7681 tgttgttgaa cttatttttc ctaatgtttg tagataatcc atacattttt ttcctaacat
     7741 atttacctcc taaaaatata atttttattt aagctaagat tagcttttat acttataatt
     7801 tttgaacttt caaataattt tgtatgtata gtaaaatact attttagtat taataatgat
     7861 ataaaaacat aaagtatgta gacgaagaac agaagtttta agttaaaaat atgaaatatg
     7921 atttttatta ttatagtatt tttctataat aatatctctt tccagttaga ggataatttt
     7981 caagtgaaaa tactttatgg tatccaaatt tctcataaaa tttaggtgct tgaaaactga
     8041 atgtatctaa aaaaacatat ttacaacctc gttctttagc aattttttca gccttatata
     8101 aaagttgact tccagtacct ttatatctaa gcttctcact aacccataag tattttatag
     8161 aaagccaatt accatgagta acacctataa aacctgcaac aattttacct gctttatctc
     8221 gaatatatat tcctaaatcc actgggtttt tatcttcaag tctagcaaga ttatatttta
     8281 ataatccttg aaaaatagta tcttcatctt gtttttctat tttttctgta atttctaatt
     8341 ccatatttga actttctcct tgtataatct tttgtatatt ggtatttttt aataaaatca
     8401 gtagaaagaa tatagtttaa cattattcta ata
//
LOCUS       DAEMHZ010000157         8203 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000157 DAEMHZ010000000
VERSION     DAEMHZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.158"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..459
                     /locus_tag="KPK72_003331"
                     /pseudo
     CDS             1..459
                     /locus_tag="KPK72_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            777..1031
                     /locus_tag="KPK72_003332"
     CDS             777..1031
                     /locus_tag="KPK72_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490520.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1067..1390
                     /locus_tag="KPK72_003333"
     CDS             1067..1390
                     /locus_tag="KPK72_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490521.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKIISTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1663..1815
                     /locus_tag="KPK72_003334"
     CDS             1663..1815
                     /locus_tag="KPK72_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490522.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2009..2931
                     /locus_tag="KPK72_003335"
                     /pseudo
     CDS             2009..2931
                     /locus_tag="KPK72_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
     repeat_region   3198..3556
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3198..3226
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            3747..3899
                     /locus_tag="KPK72_003336"
     CDS             3747..3899
                     /locus_tag="KPK72_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490523.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4019..4833
                     /locus_tag="KPK72_003337"
                     /pseudo
     CDS             4019..4833
                     /locus_tag="KPK72_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            4885..5046
                     /locus_tag="KPK72_003338"
     CDS             4885..5046
                     /locus_tag="KPK72_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490524.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5375..5839)
                     /locus_tag="KPK72_003339"
     CDS             complement(5375..5839)
                     /locus_tag="KPK72_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490525.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIITL
                     LFIVLLIFLVNKGILPIAIPYKNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(5975..6292)
                     /locus_tag="KPK72_003340"
     CDS             complement(5975..6292)
                     /locus_tag="KPK72_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490526.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6575..6871)
                     /locus_tag="KPK72_003341"
     CDS             complement(6575..6871)
                     /locus_tag="KPK72_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490527.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(6890..7303)
                     /locus_tag="KPK72_003342"
     CDS             complement(6890..7303)
                     /locus_tag="KPK72_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490528.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKDIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7287..7520)
                     /locus_tag="KPK72_003343"
     CDS             complement(7287..7520)
                     /locus_tag="KPK72_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490529.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7739..8113
                     /locus_tag="KPK72_003344"
     CDS             7739..8113
                     /locus_tag="KPK72_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2490530.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
BASE COUNT         3327 a          861 c         1302 g         2713 t
ORIGIN      
        1 gctaaattac aaagagaatt atcgagaaaa acaatcggta gtttaaatag aaataaagca
       61 agattaaaag ttgcaagact tcaagaacat atagctaatc aaagaaatga ttttctacaa
      121 aaactatctg ctaaattaat aaaagagaac gatattattt gcatagaaga tttacaagta
      181 aagaatatga ttaggaatcg taaactatct cgctcaattt ctgatgtgtc atggtcagag
      241 tttattcgtc aattagaata caaggctaat tggcatggta gacaaattgt aaaggtgggt
      301 aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaatga ggaagtgaag
      361 gacttaaata taagagaatg gatttatcct agttgtaatg aaactcacga tagagatata
      421 aatgcaagta taaatatatt aaaagaagga ttaagactaa attgcattca aaagctaaag
      481 tggaaaccta gtgaatatat gaatttagaa gttaatgaga gagcattagc agccgactca
      541 atacttatta aaattgaaga tgaagaagaa gcaatgaaag aagctgaaag agagaaaaag
      601 agagaaaaag agaggtagaa gaaggtagaa tttaaagtta aaatagtcac attgctaata
      661 tatgaaaatg ttacaatttt ggaaatagat ggatatatat gttgtatagt ataatgtaga
      721 taatatatca ccaagtaaaa aacaacaaat aaatctaaat tatgaggggt gtatttatag
      781 aaaataaaga tattaaactt acaatatgta tatttgcttt tgtaattgga ataatagtgt
      841 tattttgctg tataggtcta ggtcaattta atatggattg tattataaaa caaaatggtg
      901 ggtcaatagc taccgaacaa tacaacatat atttagaaca atctatttca caatatagaa
      961 attttggttc tatactggtt ttactaggag gaataggaat actgtttgat aaaagtgtca
     1021 gaaaatacta aaaaagtata tttttattta ggatgtgatg aatgatatgg caagagatgt
     1081 atttagtgta aagatgacac cactagaagc acagaaaatg gtagaaaaaa atttggaagc
     1141 agatttagtt tctgttgata tttataacct agaaaataat aagtctataa tagttacaat
     1201 ctttgaaaaa tattatgcaa gaagcaatag tgatgcaggt ttggtagtga tttgtgaaaa
     1261 tactacagga aaaacacttg ttaaaattat ctcaactggt agtgcaattg gactatttca
     1321 gattgattgg ggaacaggta ataatttgat taaaagagtg aaaaaaatac ttattgatta
     1381 tattatttaa ttattttatg tagatattct taggttggta tggaatttgt actaacctat
     1441 taagtaatca ataaaactaa ataaacatag aaagcactta caaatatgta ggtgcttttt
     1501 attgaaaaaa tagaaaataa aataaaaagt aagatatagg aaaaatatgt aagaactata
     1561 tgttataata attgtagcaa gaagatttaa tctacaattt atagagtgga gttcatgtat
     1621 taaaaaatta tctcccaacg ataagaaggg aggtgaatat acatggataa ttttttattt
     1681 aatgtgttag ctagtgtaac agctagttac atagtttatt tgattagcaa gttaatcaaa
     1741 aaagtaaaaa gccactctac cgcaaagagt gacttaaaga ttgatataaa ttttaaatat
     1801 catcgtaaaa aataactttc agttatagaa cttcactcta ccgcaaatag attgtagttc
     1861 ttcttgcttt tattatacca caaattagaa aaaatattgc atataatatt ttttatagtc
     1921 aataaaaata tgaaattttt tatacacaat gatgatgaat ctaactagat gaataaattt
     1981 aattgtaaat aagaaagtac ttacttaaat ggtaggtgct tttgctttgc caaattatgc
     2041 tataatattg agtgagatga gtaatttgtg gggaatttta caatgtggat attagtacca
     2101 ataataacaa tagtcttact tataatagct gtttcttcta tgcaatatat tttagttatg
     2161 atagcttttt tgttgattat atattctttt atagaaaaga aaatagttat gggtcttgtg
     2221 tcagttcttt ttttcactta ttcaatttat ctttgtgcaa cttgggaaga taaatccttg
     2281 atagcagata ataaagttga gactgttaag gcacaaagag aaactgtaga aagagaaaaa
     2341 gaaatggaaa gaagaagaat acaagaagaa gtagacaaag aaagatatat tgaaaaacat
     2401 ggaatggaaa tatcagaaaa tgatttaaaa gttaagctag aggctttagt acctcaagaa
     2461 tataatggga agaaatatga attaaaagta ggaaaattta aaagatatag tatgtatttt
     2521 gatttaactg tacaaaatga aaaatttagt aattctgaag aatgtaaaaa atttgtaaaa
     2581 gaaattgcta atgatcttaa aaaaattaaa ataagcaagg tgtactttaa gtttcattca
     2641 aaagatgacg gtggtatata taattctgtt tatattgatt attttagaaa catacaaaat
     2701 aatgtagata atgttgaaaa tttagaattc aatgaatttg aacttaaaac cgaagaagaa
     2761 gagaagcgag aacaagagaa aatagaacaa gaaaaaaaca gttataataa ctacattcaa
     2821 aacagagtgg ttgacccact agatagaata aagaaactaa aagagttgct agattcagga
     2881 gcaattacac aggaagaata taataaaaag aaaaaagaat tattagaata gataatatag
     2941 taagcactta caagtatgta ggtgctttta aatttacaaa gtattccatt ttaattttat
     3001 agtttagatt ttatgatata ataaaaatat agaagttttg cagtgtgcga tatttgttac
     3061 aaagtagggt ttaatacttg aaatctaaga tgttgagggt gcgtgataag tgttatcaat
     3121 tgcactattg cccgctcact gcaattttaa gagtattgta tatatgtagg tattggaaat
     3181 gctaagttta ttttggggtt ttagattaac tatatggaat gtaaattaga ctattttaca
     3241 aacaaactta acgagtacaa tgagttttag attaactata tggaatgtaa atcattgaat
     3301 ctgcttatta atactatatt tacagactaa gttttagatt aactatatgg aatgtaaatt
     3361 tgataaatat acaccagtag aagattagat taataggttt tagattaatt atatggaatg
     3421 taaatatagt attgacatgg ttaggctacg tgttaatgtc ttgttttaga ttaactatat
     3481 ggaatgtaaa ttgctgtgat ttcactaata aaacaattaa taattgtgtt ttagattaac
     3541 tatgtggatt caaaattaaa taaacaaaga aagcacttac aaatatgtag gtgcttttat
     3601 tctgctcaaa attggtcggt tgggtaaaat aattagaaaa aattagtaaa aacctattga
     3661 ctgtaactcg ttacaatatt gttattaatg taacgagtta cagaaaagag gtgaataaaa
     3721 tagcaactaa aagtagggca gagtatatga aaaatcgtcg aaaagataaa agaggtttta
     3781 gtgtactttt agacaaagaa aagttagata aatttgatga agtattagag gaaaagaatc
     3841 taaccaagaa agaatggcta gaagaaaaaa tcgacgagga actggaacaa aaggaataaa
     3901 aaataagggt cactcccacc gaccaaagtt tgagtaaccc ctatgacgta tactatcgta
     3961 tatcaattat agtatatgtc attccttaaa aaaatcaatt attaaggagt gtaatattat
     4021 gaaaaatgaa ttaatgatgt ttgaaggaaa agagattgaa gtatttgaat ttgaaggaag
     4081 aattttattc aactcaaaag atgtagcaaa ctgtttagac ataaaaaatg ttaatgagaa
     4141 tataatactt atgaatgaaa agcaagttgt aaagttaaga aattcggata tcagtaatac
     4201 tgacatccga aaacttaata atgctggaga aaattttctt actgaaagtg gagtgtataa
     4261 attaatcttt aaatcaagaa aagaagaagc tgagagattc caagactgga taagtgatga
     4321 agttttacca agcataagac aaactggtgc atacataact aataatgcaa taccgaaaag
     4381 ttgagagaaa aagcaagtga gattgaaaaa ttacagttgg cttataacag tacatctatg
     4441 ttaaaagaat tgctagatga tgcaggcttt gacaataaat caaaactatt aacagctaaa
     4501 acattatata agaaagcaga aattgattta ccaatagaga taaacgaaga agaacattat
     4561 tttgatacaa aacaaatagc atctaaactg aaaatatatt ctaagagtaa taaaccagct
     4621 cagatggctg tttgtgagat tattaaaaag attgatttag aagatggtga agttaaaggt
     4681 gtttgggaaa ctaatggttc ttggactggt actgtaaata aatatacaaa gagtgtaata
     4741 gataaggtga gaacttggat agaggaaaat aatagaccta ccaagattgc aggtgagaag
     4801 aagaattatc atgtatttta caaaattgag taaatttatc agttgtatta aataatttag
     4861 tttagttttg tggggattaa tacaatgtgt gagaatttac ttgataatat gtatattgaa
     4921 aagagaaaag aagaatatag aattaaactt ttgaaaataa gagaaacaga tatagatata
     4981 tataataagt tagaaagtat agtatataaa ctttctgaga aaaaattaga gaaaaataat
     5041 aaatagacat ataaagcact tggatattct actgtttcaa gtgctttgtt tggtataaaa
     5101 tggtataatg gagatagaga attatattaa cccaaaaatg gagaaaatag agaagatgat
     5161 ttagttgacg ctttatcagt aggagtactt gcaggtaaga cagaatctct agtacgatta
     5221 gttggagatg gaataagtga tactcaaaaa agtttaataa ataaaagaga atttagaaat
     5281 ataactcaaa ttggtggaaa tggaaatgag aaagcattta gtgaagtaga aaacttagtt
     5341 aaatagatta gaaggtagca actagagtta gttgttacct tcttttttta tactttctct
     5401 ctctaagtat tttctttttc atatcttcta atacatctaa tgcagtagta taaaattgta
     5461 tattattttt ataaggtata gctattggaa gaatgccctt gttaactaaa aaaataagaa
     5521 gtacaataaa taaaagtgtt ataatcattc taataaaata attagtgata tctgataaat
     5581 caaatgttaa gttaattgtt gtagaaaata caacagccat aatagatata tacaaagcaa
     5641 aaatggggtt actatttttt tgtaactgtc ctttaagcct agattgctct atttctatat
     5701 cttgaatact ttttatggaa tccatttcac tataatactt ataaactttt aaataatatt
     5761 ttttatactt gtttaaagct tcttcattat ctttaaggtc tttgtcactt aactctgatt
     5821 taacttcccc aaaatctata aaatccctcc tacaatgtta tataatatac tttaatttat
     5881 agcatattta agaacttata tcagcaattt gtcgaacaat tttagctaaa aatagagtat
     5941 aatgataggc ttattattta taagatatta ttttttatat agttatatat ttctttctaa
     6001 ttagataata taatgctgtt gttataatgg ctactaatat tattgctaat attgtaatat
     6061 ctaatcttct tgtaaataag cctattgcta tatactcaag acctgttaca tagcgataaa
     6121 gtacttctag tttaacagtt tttttcttat ctatatttcc atatattgat tctaatttat
     6181 taaaatattt tttactacaa aaagcagttt taggtttaat taaatatatt attgtagttc
     6241 ctattataac tattattcca ataagtataa gattgatatt tccaaaatcc atagcagata
     6301 cctcctatta aatttgcttt tcttaatcaa agtatatcag taacacaagt acaaagagta
     6361 tctaaaatat tacaaatatt aacaggatag ataaaactat agtaaaaatt gtaataataa
     6421 agcgaacgaa aattatcata ctaaaacata agacatgtta taattgtatt agatagaaga
     6481 ttaacataca ccaaagcact ccttataata aagagtgctt atttttttga aattcatcaa
     6541 tatataaact atcaagaacc ttattcaaca caccttaaaa tcgatttaaa tagctctttt
     6601 tgataacata atttatatga tataataaaa aaatagaagt gtaccaccaa tacacttcta
     6661 tagctataac taagcattct cttgctgggg gagtgctttt ttcatttctc caaacaacat
     6721 tatttacttc atcagtaatt atcaaatcaa tctcattatc tttcaaaatc tctgttgcag
     6781 tacaaattag attatctaag tcaaaacttc gagttatctc tagttgtttg ttatttactt
     6841 ctctttttag tatataagtt ttaaaataca tttatatcac cccttgtatt tactcttttt
     6901 tctctaaaaa tatattgata agtaaatatg caacaaataa aaaaatctca atgacacata
     6961 gtgttatagc tataactaga agtgattgat ttttccatgc actataagct atgatgaaaa
     7021 gaaatgtaaa agtaaataat gtataattca taattttaac taagatatga cttgttttca
     7081 ttgttaagta caagtctctt tcatctgtac tatcgtgtaa ttcttcaaca atgtcttttc
     7141 ttgaaaaagc attacaaaaa ttaaagattg ctattacaat taaaagaaat gatgaaatta
     7201 ggaatctttt atcttttaaa atgcaataaa taactaaact agcacaacat aacacacaaa
     7261 ctataatacc cactataaaa cttcgtttac tctttacttt catgtttttt atcctccatt
     7321 tctttatttt cttttaaaca acatagttct tcaatcgttg taccaaacac ttgtgccata
     7381 cgataggcaa gcataagaga agggctatat tgctcttttt caatagaaat aattgttctg
     7441 tttgatacat ggactaaatc agcgagttgt tgctgtgtta gacccatatt tgttcgaaat
     7501 tctcgtactt ttgttttcat tccataacct ctttctatat gaagctaact tcatgtgaag
     7561 ttagcttcat aatatcctaa aatgagtaaa atgtcaatct cattagataa gtattataaa
     7621 acctaaaaga ataatatggt aaaaattaga tggaattatt taccttaatt ctcttgtttg
     7681 taaaacttta ttgatataga atttaacata tacataaatc ggcaaagggg cgcgaattat
     7741 gaaaattagt aaattaccag aagcggaatt aaaagttatg agatatattt gtgaagctaa
     7801 caaggtatta acttctaggg aattagttgc tgcaatggaa gaaaaatatg agtggaatga
     7861 atcaactaca tttacagttt tgaaaagatt agagcgaagg gagtttttaa gtacagagaa
     7921 aataggcaaa cttacacatt ataatatact agtaaaagag aaaaaatatc taaggtttga
     7981 aacaaaggaa tttttgaaga acatacataa aaattcaatt tcaagtttaa tgtcagcgtt
     8041 acatggtgaa gacgacgaag tggatgaaga taaattaagt gaactcgaag aatattttaa
     8101 gaatttgaaa taagaaaatg acaaattgat acataataaa taaaatgaag gtatgaaatt
     8161 ataacttttt ctaccttcat tttgtttatt attctgaaca tgt
//
LOCUS       DAEMHZ010000158         8188 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000158 DAEMHZ010000000
VERSION     DAEMHZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.159"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            494..1489
                     /locus_tag="KPK72_003345"
     CDS             494..1489
                     /locus_tag="KPK72_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2490531.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1467..2330
                     /locus_tag="KPK72_003346"
     CDS             1467..2330
                     /locus_tag="KPK72_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2490532.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTIIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2327..3121
                     /locus_tag="KPK72_003347"
     CDS             2327..3121
                     /locus_tag="KPK72_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2490533.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3406..4785
                     /locus_tag="KPK72_003348"
     CDS             3406..4785
                     /locus_tag="KPK72_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2490534.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIF
                     KYKNRYKYM"
     gene            5233..5946
                     /locus_tag="KPK72_003349"
     CDS             5233..5946
                     /locus_tag="KPK72_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490535.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(6218..7756)
                     /locus_tag="KPK72_003350"
     CDS             complement(6218..7756)
                     /locus_tag="KPK72_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2490536.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENGQYLIKASDYQEQLIANNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         2892 a          942 c         1267 g         3087 t
ORIGIN      
        1 aattcaaccc tgaagaaata agctaaatgt attagtatat aaatccatac ttgaacataa
       61 ataaccttta tgatatgata tcaataataa tattatgaga ggagtgcctt gatgtcggag
      121 ttatataatg gtaaccaaca attaaatagt tggagacaca tatattctgt ataatcagaa
      181 atttcaagtg tatttttaat acatttaatt tttgtgtctt attctgcctt tatagataac
      241 tgacatatga caacaaatag tatttaaaat atatattact ttgttaaatt atggtgtagt
      301 aggctttaat ctgctacact tttattaatt ttgtatagta ttattttgat aaatattttg
      361 aaatagagag cattaatttt tctagtccac aggagttttc tgtttttgtg ctgttaaata
      421 taagcacaga aaaaggcaga tatatacctg tggacttttt ttatataaat tatgaaatat
      481 ggaggtagtt aaaatgagta taattgaact taatgaactg actaaatctt atagaagatt
      541 tgagaaaaag gcaggattta tggggagtgt aaagtcttta tttaatagga aatatacaga
      601 aaaaacagct atatcgtcat ttgatttaaa gatagaagaa ggagaatttg ttggattaat
      661 aggtcaaaat ggagctggca aaactactct tattaagatg ttaactggta ttattgcacc
      721 gtcttctgga gaaatatctg taatgggtta ttaccctaac aaattagaaa ataattttaa
      781 aaagcaatat gctgttgtta tgggccaaaa aagtcaacta ttctttgaat tgacaccagc
      841 agatacgttt cttttattta aagaattata tgatatatct aatgaagaat ataaaaaaaa
      901 cttggattac tttatcgaat tatttgaggt atctgactat ttaaatgttc aagttaggac
      961 gctttcttta ggagaaagaa tgaaaatgga actaatagta gcccttctac ataatccaaa
     1021 aatattattt ttggatgaac caactatagg cttagatgct atagcccaaa aacaaattag
     1081 gtatttccta aaagaagtaa atgaaacaaa aggaactact attatactta cttctcatta
     1141 catggaagat attaagagtc tctgtagtag aagtattgtt gtagaaaata gccgtaaaat
     1201 atatgatggt tctacagaga aactatttaa tcattttcaa aaatataaaa aaataacagt
     1261 tagtttttcc aatgaaacta acattgtatt tccaaaagat tgtaaagttg ttgaacaaac
     1321 aaatcataag atatcagtaa ttgtgccaaa agaaagagga aatgaaacga ttaaatggat
     1381 ttttgataaa tatgatgtta aagatgtaat gattgaagaa gaagatatag gtagtattgt
     1441 agagaggata tataagaaag gaagtgatga gtattatgag caactataat gaagcaagtt
     1501 caaatgattc aatgttttat aaatttaaac agagtattag aaggtattat gaagtagcca
     1561 aaattaactt taaagtgcaa cttatctgga gatttgatgt ggtggttaat atggtgttta
     1621 cgattgtaaa aattttattt gcttatattt tatggaaagc aatttttggt gaaaataaaa
     1681 tagtctctgg ttttacattt aactctatgt tgtcttacta tataataagc tcatttttag
     1741 caggtattga tatgtcaaag agtaccagtg aagagatatg taatcgtgta aaattgggca
     1801 ccttttcaaa gtacatgatt attccaatta atgttcaaaa ttattttatt gctaaaaatt
     1861 ttggaatgac atgtttttat tttgtattta atatgatttc tgcatttgta tggattttta
     1921 tatttaaaat agattttgcc tttacatcaa atccagttca aatattaatt tcaattttga
     1981 tgatttttct agggtttatt tttatgatga aactccactt ctttattggg attttgtcat
     2041 ttaaatttct tgagattagt ttttttagaa tgataattga aaatattgtt gaatttatta
     2101 caggaacaat aataccactt gtactcttac cagaaactat agtaacagtt atgaaggtat
     2161 ttccatttta ttatacaaca tatttgccat ctatgttact aataggtaga aacagcaatg
     2221 aggcaataca aggattaatt attctagcat tatgggtttt atttttttct gtagtaaata
     2281 aagttatgta taaaagaatg agaacattat ttgatggggt gggtatatga tgaaaaaaaa
     2341 tttgaaaata atatttatgt tattaaaaat gaaattatca aagatgatgg tatttagatt
     2401 cgattttttt ggagcatttt ttgtagatag ttcattattt atattacaac ttttaatgtt
     2461 taatagtata tattctcatg tagactcaat agggggatgg caacaaggag agatgttaat
     2521 atttatagga actttctcat taataaatgc gataaatatg acgattttct tttttggtat
     2581 ttatgatatt ccacgaaaaa tccaggaagg agaattagac tactatatta caaaaccatt
     2641 agatccacta tttaggttaa catttgaaaa tattaatatt ggttcatcac cattaattct
     2701 agcaagtata cttattgttt tatatggaat atctgagtta aactcagaga taactttagt
     2761 tatgataata atatatattt tcatggtgtt attgatgaca ttactttttt atgatgtatg
     2821 tatcatactt agaacagttt cattttttgt tatatcatct tctgcgataa tgagattaga
     2881 agataattta ttgccaatga atatgaaaat tcctggtgta atatatgaag gtgtttttaa
     2941 agtattattc tatttaatct taccatatgg aattatgtca acaattccga ctcaaatttt
     3001 ggctgggaca ataacaatta atggattaat atactcaatt ttaattgtat ttttgtttac
     3061 attgtttatg tggagatttt ggaagttagg tatgagacat tataaaagtg caagtagtta
     3121 ataaattagt aaaaatagac aatgtataac tatatataaa tacgtttcat atgacactaa
     3181 actatgtgaa caagtatttg cgctaaaggc actgaaagct ataaaatgat taaagtaagg
     3241 atttaaaagt ttctttagta gaaatataat tgtgtaaaaa agcaagatta attaaaacta
     3301 tatccaaata attagatata attcttatgc agaaaggagg tctgtaaatg tctaattgtt
     3361 tggaatatat tttaaaaata attaatgtac agaaaaataa acaaaatgag tgatgtatat
     3421 tattcagaaa atcatgaaca agataatctt tatgagcaag ataatttact ggcacaaaga
     3481 tttaatttat ttactctatt gaagtttgca tttcctacta tttttatgat gatttttatg
     3541 gggttgtata caatcgtaga tactatattt gtatcaagat ttattaatac aaatgctcta
     3601 tcagcaatta atattgtatg tccaattatt aatcttatag ttggaattgg aactatgctt
     3661 gccactggag gaagtgctat tgtagcacgt aagatgggaa atggtgatat tgagggagca
     3721 aataaagctt tttctttaat cgtattatca ggagttgtaa ttggggtaat aattactggt
     3781 ataggtattg tatttatgga ttatatcatt atatggttag gagcaagtga agttttaatt
     3841 aaatattgtc gttcatatct ttttattatc ttggtttttg cttctgcaaa tatactacaa
     3901 gtattatttc aatgtttatt tgtaacagct ggaaagcctc aatttggatt atggctagta
     3961 ttaggtgctg ggtgtgctaa tataattctt gactatgtgt tcattgttct gttacaaatg
     4021 ggtattagtg gagcagcact tgcaactgga gtaggatacc taattcctac cgtttcaggt
     4081 atggttttct tttcaaaaaa taaagggtta cttaaatttt gtatacctag actagataaa
     4141 agaatattat ttgaagcttg ttttaatggt tcatctgaaa tggtgggtca actatcaaca
     4201 gcagttacta cttttttatt taatgctatg atgatgaaat taattggtga agatggggtt
     4261 gcagctatta caataattat ttattctcaa tttcttttaa cttcgcttta tataggtttc
     4321 tcaatgggtg ttgcaccaat aataagttat aactatggaa gtgaaaataa tacgcagtta
     4381 aatcatgttt ttaagatttg tatattattt atttcagtag catctttatt ggtatttatc
     4441 ttttctatat catgttcacc aatcattgta gagatttttt ctcaaagagg aagccgagtt
     4501 tatgaaattg caaggtccgg attttatata ttttctttta gttttatttt ttgtggatat
     4561 aatattttta cctcatctat gtttacagca ttatcaaatg gaaaggtgtc tgcaatatta
     4621 tcttttctac gtacatttgg atttataact ataggtttat tgatattgcc aaatatatta
     4681 aatataaatg ggatatggtt agcagtacca agtgctgaga ttttaacctt atttttaact
     4741 attttttgta tatttaagta taagaataga tataaatata tgtagatatt aaataaatag
     4801 cttagataga tattttatgt tttaataagt aattcaatta gtggtagata tatactattg
     4861 aaaattaaag ttataattga tataaagaat taataaaaat aacatataaa agtttaaaag
     4921 ggagttgaac ttaaagtttg attagaaaaa gtcaatttta agacaactcc ttttatttta
     4981 ctattgtatt aatctgtatc tgtaagtgta ttagtctaat attataaact ttggatgaaa
     5041 ttaattttct ataggtttta atgtatcata cactttattt taaagaaact tatcttaatt
     5101 ttcatagtga ttgaatttgt ctattagaga tgttactatt agtaatgtaa aagcaagagt
     5161 agtgaatata aaaaacaaga tagtgatact gcaagattag aatataataa atttttatgg
     5221 agagatttca atatggatac atgttttaat aaaaaaatat tacttgttga tgatgaaaag
     5281 gatatagtag atttaattga agaagtactc ataaatgatg gttttaaaaa cattattaaa
     5341 gcttataatg gtctagatgc tatttcgctg tgtaagatag catgtccaga tgttgtcatt
     5401 cttgatataa tgttaccaga tatagatggt atagaagtgt gtaaaaaaat tcgagagttt
     5461 tcatactgtt ctattctttt tttatcatca aaaaatgatg atattgacaa aatactaggt
     5521 cttagtagtg ggggagatga ctatataaca aagccattca gtccaagaga aattgctttt
     5581 cgagttaaag cgcaattacg tagacaacag tatcaaagca ttgtaccatc agatagtgaa
     5641 gttattaaaa taggagatat tactattgat atagaaggaa accgtgtata taaagataga
     5701 aatgagattg agttaactgg aagggagtat catcttttaa gctacatggc tcaaaatgta
     5761 aataaaatta taggaaaaga aagactctat gaacaagtat ggggagtata tagtagtatt
     5821 tgtgacaata caattatggt gcatatacga catataagag aaaaaattga agataacccc
     5881 tcaaatccta aaatacttat aacagttaaa ggactaggat ataaacttgt gaatagaatc
     5941 gattagaata gattttaata ttaatgaaga tatctcaagt tgaattttta gttaatgagg
     6001 cattctttta tgggtgaaat caaatgtatt ttcgttatct caacaatgaa gaagaggtct
     6061 atctctattt tgagatagcc tcattaagtt tatatcctgg taatgtactt tatgttctaa
     6121 taaaattttt ttagtttatt tgcagtgaaa cattaactag aacattaaaa tttgcattga
     6181 aataccaatt aaataatatt gtatattgtg tataaactta tactaattgt ttttctatat
     6241 taaatacttt atcaatccaa cctttataaa atttttcttc atgggaaact aggattatac
     6301 ttcctctaaa ctgtttcaaa gcattttgta aagcatcctt tgtctctgca tcaaagtgat
     6361 tggttggttc atccagaata agaaaattac aaggagataa catcatacaa cataatttaa
     6421 ccttagattg ttcaccacca cttaaagtag atacactcct tgaaacatgc tcttccttca
     6481 caccacatct ggctagatga cgtctaatct cttttgtatt taattttgga tatttatctg
     6541 ctactatttg aagtggggtt ttatcaggat tttcccattt taaatcttgc tcataataac
     6601 caattttaac ttgttctgaa aactggaaat ctccagatat acgaggaata tctttgacta
     6661 atgtctttaa caaggtagat ttaccgattc cattaaaacc agttataact attttttgac
     6721 ctccatcaac agaaaaattt aattttggaa gtaaagaata gtagtatcca acttcaagat
     6781 ttgttatagt aagagcattt tgagccgata tttcaatttc tgaaaattga atatttggtt
     6841 ttcctgtaaa actaggagga gctattcttt ctattttatc caattgtttt tgtcttcctc
     6901 ttgcaatttt tgaatttact ccagctttat ttttacgtat aaatgtttct tctttttcta
     6961 ttttcttttg ttgtgcttga taccttctaa tataatcttc tcttaaatgt gatttttgtt
     7021 taaggaactc tgaatatttt ccatgatatt tcttaatcat gccaaattct atatcaagaa
     7081 tacctgtaga aattttttct aaaaagtcaa aatcgtggga aactacaata aatgccccat
     7141 taaatgtatt taaataattt gagagccatt caacatgttc tttatctaaa aagtttgttg
     7201 gttcgtctaa taatagaaca tcatggttag aaagtagtaa ttttgctaaa attacctttg
     7261 ccctttgacc accactaagc tctccaacaa cacgattcat tcctattgaa tctaatccaa
     7321 gaccatttgc tattttattt atttcattat ctatagaata gaaattattt gctattagtt
     7381 gttcctgata atctgatgct ttgattagat attgcccatt ttcagatata gcactttttt
     7441 gatagagtag attcatttcc ttttctattt tataaagttc ctcaaatgca gtgtgtaaat
     7501 agagtgatat tgttgtatct ctattgattt ctgcatattg gtctaaatgg cctatattga
     7561 tattaggttg ccacttgatt tctcctgaat ctggaacaac ttctcccaat aaaattttaa
     7621 ttaatgtact tttacctgta ccattttggc caactacacc tatatgttca cctttgaata
     7681 aatcaaagtt agctttctca tacagtgatt tatccataaa acattgtgat aaattagtta
     7741 cttttaataa actcataatt caaactcctt ttcttttatt aaaaaattta tggaaataaa
     7801 aaaagcagag gaatggcatt gtaaaatgcc gacctctgct tttggaaaaa caaaaggtca
     7861 tagacaagca ttgttgtcta tgaccagaaa atctatatta tctatcggca aataggtaat
     7921 tttaaataag catagccaaa aaactatact aaacctatat ttgccgatga agatagaaag
     7981 ctttaagctt tcagattaac ctctacacaa cgcttcatcg tatacatttg tgtagagtta
     8041 agttttctaa caagtttaac taattctata taaatcataa ttattcacat cctaatctta
     8101 gtattaattc tagtttatct atatttaaat tgtacttttg tactttgtgg attatcttat
     8161 ttatttaaaa tacgttttat taattata
//
LOCUS       DAEMHZ010000159         8175 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000159 DAEMHZ010000000
VERSION     DAEMHZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.160"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..280
                     /locus_tag="KPK72_003351"
     CDS             <1..280
                     /locus_tag="KPK72_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2490537.1"
                     /translation="KLSRSISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNK
                     CGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            397..600
                     /locus_tag="KPK72_003352"
                     /pseudo
     CDS             397..600
                     /locus_tag="KPK72_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            924..1292
                     /locus_tag="KPK72_003353"
     CDS             924..1292
                     /locus_tag="KPK72_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2490538.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            1409..2206
                     /locus_tag="KPK72_003354"
     CDS             1409..2206
                     /locus_tag="KPK72_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2490539.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPTHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            2605..3495
                     /locus_tag="KPK72_003355"
     CDS             2605..3495
                     /locus_tag="KPK72_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2490540.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4258..4701
                     /locus_tag="KPK72_003356"
     CDS             4258..4701
                     /locus_tag="KPK72_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490541.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMHALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            4873..5043
                     /locus_tag="KPK72_003357"
     CDS             4873..5043
                     /locus_tag="KPK72_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490542.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5416..6537
                     /locus_tag="KPK72_003358"
     CDS             5416..6537
                     /locus_tag="KPK72_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2490543.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            6742..7230
                     /locus_tag="KPK72_003359"
     CDS             6742..7230
                     /locus_tag="KPK72_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG2490544.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7316..7723
                     /locus_tag="KPK72_003360"
     CDS             7316..7723
                     /locus_tag="KPK72_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490545.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            7724..7942
                     /locus_tag="KPK72_003361"
     CDS             7724..7942
                     /locus_tag="KPK72_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490546.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3224 a          760 c         1446 g         2745 t
ORIGIN      
        1 taaactatct cgctcaattt cagatgtatc atggtcagaa tttattcgtc aattagaata
       61 caaggctaat tggtatggta gacaaattgt aaaggtaggt aaattctttg caagttcaca
      121 aatatgcaat aaatgtgggt ataaaaacga ggaagttaag gacttaaata taagagaatg
      181 gatttgtcct agttgtaatg aaactcacga tagagatata aatgcaagta taaatatatt
      241 aaaagaagga ttaagactaa taacaactca aaataaataa cttatgtaag aaccgtagga
      301 actacgggga tagcttgtgg agaattagta agacatattt tatatgcaaa attctatgaa
      361 gcaggaaccc tgtgacttta gtcatgggag gttcagtatg tgaaatctaa tggaaaaagg
      421 aataggaaga tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat
      481 atgaaaaaac attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg
      541 attgctatta ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa
      601 ttttactaac agaataatct atatcagata ctttgctaaa cttatataat atattttact
      661 aaatttatat taggtagttt actacttgtt tattgaattt aaatacaaag aaatacaggt
      721 gctaatccct gcccattaaa ggaatataag cataacacaa aagtagaact tatcaattct
      781 gttacgggta tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa
      841 gttagtgttg attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta
      901 gaatttgtag aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac
      961 aagatgctgt taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata
     1021 caaatgagca ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat
     1081 ggaatggtaa agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg
     1141 tttatgcaat aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg
     1201 ataagattat ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat
     1261 tagataaaca tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt
     1321 tgataacatt tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta
     1381 aaaaagtaga atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg
     1441 gaaagtatat tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt
     1501 gccaggacta acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa
     1561 tcataatgta ttggtatggg acgctcctac tcatggaaaa tcaagacctt acttagattt
     1621 ctcatattct aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa
     1681 ggttgtactt atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata
     1741 tccaaatatg gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta
     1801 tagtaagtca gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga
     1861 taaatttttg cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag
     1921 tatgctgaaa atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg
     1981 atttgctggg ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt
     2041 ggttggagat aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa
     2101 gactgggtat ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc
     2161 taatgaacta aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac
     2221 atacccatta ggtaaattat gtagtatatg tgtttacaga tggttataga aataaatatg
     2281 taataaacta cttaacactt aaaaagtgga tttgtccaga gcataataca aagcatgata
     2341 tatataaatt tttaagcgaa atacatcaaa agaaggatta gaattagtgg agacagaaca
     2401 gctattatag attataaata aatagtttta aaggagaggt actacgaata tagaggaagg
     2461 taaatcatac gttattcaag tattataaat gattagttta aaagtactat cggataggag
     2521 tctccatatt taaaccttag taaaggtagt taaagtagta gtttaaaaac taaaaaatta
     2581 tataaaggaa ttaggtataa gaaaatgatt gagagtatac cagcaaaaca aattttacaa
     2641 aaagtgaaat ttgacaatac aagatggttt ggaattgact ataatatgaa tctctatcgt
     2701 ggttgttctc atggatgtat atattgtgat agccgtagca caatatacaa catagagaat
     2761 tttgacaaag ttagatataa agaaaatgtg atagaaattt taagtaaaga attgcgttca
     2821 aaaagaaaaa aaggtgtagt aggcattgga gctatgtctg acacatataa tccttttgaa
     2881 aagcaacttt gtattacaaa gcaagcacta gatttgattt cggaaaatca ttttggagtg
     2941 tctattgata ctaaaagtag tttggtggtt agagatatac cgattttgca gaaaattaaa
     3001 aaaaataatt ctgctattgt aaagttgact ataaccactg caaatgatga attatcaaag
     3061 aaaatagaac cttatgtaaa tccaagttct gtacgatttg aggcagtgaa agaacttaat
     3121 gattctggaa ttttttgtgg tatattattg actcctatgc ttccattttt gactgataat
     3181 aaagatgaaa tacgagcaat tgtagaaaaa gcacacaagg ctaatgcaaa atttatttat
     3241 tgcatgtatg gtgtaacaat gcgtagtggt cagagagagt ttttttatga gcatttaaga
     3301 gatatatcac caaaattggt acttaaatat cagaaaacat atggtttaaa ctatgtatgt
     3361 actgtacaaa ataaagattg ttgtgagaag ttgttaagag aagaatgtgc acgttatggt
     3421 attttgacgg atatgaaaga gattataaaa gcgtacaaga ggtcggagtc atatattcaa
     3481 ataaaatttt tttaagatag gctttggatg agatataata gtggtgattg atgtgttgat
     3541 attatagttg attgttagag aggatattat agatgaatat tagtattgat attttaggta
     3601 attgctagag ctgatattat agatgaatat tagtattgat attctaggta attgctagag
     3661 ttagtattat agatgaatat tagtattgat attctaggta actgctagag ctaatattat
     3721 agatgaatat tagtattgat attctaggta attgctagag ctaatattat agataaatat
     3781 tagtattgat attctaggta attgctagag ctgatattat agatgaatat tagtattgat
     3841 attctaggta actgctagag ctaatattat agatgaatat taatattgat attctaggta
     3901 attgctagag ctgatattat agatgaatat taatattgat attctaggta attgctagag
     3961 ttagtattat agatgaatat tagtattgat attttaggta attgctagag ctgatattat
     4021 agatgaatat taatattgat attctaggta attgctagag ttagtattat agatgaatat
     4081 tagtattgat attctaggta attgctagag ctgatattat aatttattat tagtattaat
     4141 attctaggga attatcacta tattttaaaa agtaaaaata tatatcaaaa tgaactaaat
     4201 aagtactgta agaagtttgt taaattaagg tttgaataaa gtgagggtgg agaacttatg
     4261 atagaaataa aaactataga aataaataaa aaaagtcaaa ttgacctttt aatgttagcc
     4321 gacccatgtg aagaaatgat tgataaatat ctcaataaag gaactatgca cgcattgtat
     4381 gataataaag aacttacttg tatagcagtt gtaaatgaaa tttccaaaga aatatgtgaa
     4441 ttaaaaaaca ttgctacata tgagcatttt caaaatatgg gttatgcctc aaagatgatt
     4501 tatcatcttt tagatgttta tagtaaaaaa tattcatcaa tggttgttgg tacatctgaa
     4561 agcggagttc ctttttatga gaagtttgga tttgtttatt cacataaaat taaaaacttt
     4621 tttgtagata attatccaga accaatcttt gaaggagaat tacaatgtgt tgacatgttg
     4681 tatctatcat ataactttta gataggataa tatttttagg ttaatataca taatttattc
     4741 atataattat cggaattata atttataaat tatgtataat aagagtataa tttatttaga
     4801 agctgttata ttgtattaga ttcaaataag atatataata acttgtttat tttgaatttt
     4861 ggaggattaa ttatggattc aaataataat actataaaat caactgttaa aaagggtatt
     4921 tcttttggtt cttgtttagc aatgattatt tcttatactg catggaaatc tattccatgg
     4981 gctatttttc atggcttaat gagttggata tatgtacttt attattgggt taagtatgca
     5041 tagattaaat taataaataa gtatgtaata ttgtaaagtt taagaaacaa aatgtaaggg
     5101 agtgtctcga agtgaacttt tagtttataa ggcactcttt tttgtattaa ataaaatata
     5161 ttttcattac ttaaacaatg aaaaagatga gtatctctat tttaatacat acccttatat
     5221 tcattcattt atcaacaata gttttaaaaa gaaagctatc taaaatctat tgtaatctaa
     5281 gtgcttctct ttaaagtctt aactttagta atgatatata agtttataag gttcttattt
     5341 caataatgca aattttactt agaagttcag actttggtat gaagaaatgg gtaataataa
     5401 aaaagaggtg ttgatatgaa agaaaaatta gaagaaaaat ctaagattat taaaacatca
     5461 aaagagtata aacgaactat aattgaagaa aaggacagag aatggaccaa aaaattaaat
     5521 gacatactta gtagaggtga tttttctaat gaaaaattag ataaaatcaa ggatttaatt
     5581 ccaaaagaaa tattgactag tgaaaatgtt ggagaagttg taactgaaga tgggtatgct
     5641 aagcctgagt atgatgaggt atttaaatca ttgtttactt taaataatga ttacgattta
     5701 cttgcaaatt ttataaatga tatcctcaaa gatgctccat atgctaatag gaatatcaaa
     5761 cagttcactc aaataaaaag aataattaag gttgaaaccg acccaacaat taattacatt
     5821 ggtgagaaaa gacctagact tgatattttg gctgaagatg aagaacgtaa ccatataaat
     5881 attgagatgc aaagagctct tgaagatgat tatttggaaa gagctgaata ttatcttagt
     5941 cgtgtacatg gtagaaagtt agaagaaggt aaagaatata aagaaattgg aaaaactgtt
     6001 ggaatacata tactaaatca tgtaaaatat aatcacatag aagattatgt aaattgcctg
     6061 agattaacaa tggacgggca tccagatatt ttttctagta aaactgcatt atattttata
     6121 gaacttccaa aaattcgtaa atccagttgt atagcaaaca gagttctaat atggggtaaa
     6181 cttattgata atccttctca tatagatatt agaatactat ctaaaacgga ttatgttatt
     6241 aaaagagcac ttgatagatt aaaagaatta ggtagtaatg aggactattt attaaattta
     6301 aagagaggtg cgtatattat gaataaaagt agaaatttta aagaagaaat ttttcaagaa
     6361 ggtattgaaa aaggtatcga aaaaggtatc gaaaaaggta ttgcaaaagg gaaaagagaa
     6421 gagaaattta atatcatcat aaaattaaaa aataaaggat acaatttgag tgaaatatgc
     6481 gatattatag atgatttgaa taaatcagag gttgaaaaag tctataatca aaactaatac
     6541 tatgattgag atggcactag ataaatacaa agcttcctct tttaaattta aattaatata
     6601 aaactttgtt atttaaatat ggtagatata aagagatata taaataatag gtacaaagag
     6661 gtgtataaat aaaggagata attcaaaata aaagtttgaa ccatctcctt cttatagaaa
     6721 aacaaagggg ttgatttata tatgaaattt tatactatac agactttaga attttggaat
     6781 aacaataaaa acaataaata tctatcaaat gattattctt ttatattaga agactttgat
     6841 attccataca aatggatgta tagtcaaatg acaaatagaa tatatgaatt tgatgatagt
     6901 atgatatggg tttggacgaa gagacctgat ttaagatgta gtggttattt agaaaaagga
     6961 aaaaaaggtg ttttacttga aatagaaata agtgaagaca atgtgttatt atcagaattt
     7021 gatttatggc atttaccttt aatggatatt actgtagagc tttatgaaga tgaacaaatt
     7081 gaaaagaagg taagttggga aagaatattt gacttagaac tgtgttcaaa attaaatgaa
     7141 gttaatatag aagatttgac attgcaagga gtgacatcta aagtgcctct atctaatgta
     7201 aaattaataa aagaatttgt tgcaaaataa tatttttatt gaaatttatt caaaaaagtt
     7261 atatatttat aacaagaggt gttataaata cataactttt taagggggat tttatatgtc
     7321 taaacaacaa actggtatta aatcaacact tatttcagaa atattgggaa tttcttattg
     7381 tattggagct acattagtaa ataagtattt ttttaaagga agtagtattg aatatcttcc
     7441 tataattatt tgggtattag ctatgggagc tcagtgggtc tataataaaa agtatgatga
     7501 tttaattgat gaggcatcaa aatgtatact atcaagagtt aatgatattg caataaaagt
     7561 attgtttttt tctgtagcta tagtatctat atttcttgta actttggaca taaatgtgtc
     7621 aaatttaaat ataggaatgt ctttattagt cattttgttt attcaatcct tgttgaagtt
     7681 aattctattt atatattttg acagaaaggg gatatacaat taggtgtcag tattactaaa
     7741 gacaaaaatt aaatcctata gagaaaagaa ccttatgaca caaggtgaac ttgcaaagtc
     7801 agtaggagta agacgtgaaa ctattgtaca tttagaaaat ggtcgctaca atccttcttt
     7861 aaaactggca atggatattg ctaaggtatt tgatacaact gtagaagagt tgtttgaatt
     7921 tattgaagaa aataatattt aaatttcatg tattacttat gcattagaga atgaattaat
     7981 agataaaaaa ttcaagttta tgtattagag aatgaattaa ttataggtaa aaaaccaagc
     8041 ttatagatta agaagtaagc ttatagatta gagaatgaat taattatagg taaaaaccaa
     8101 gcttatagat tagagaatga attaattata ggtaaaaacc aagcttatag attaaaaaat
     8161 gaatgattta tagat
//
LOCUS       DAEMHZ010000160         7963 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000160 DAEMHZ010000000
VERSION     DAEMHZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.161"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..166
                     /locus_tag="KPK72_003362"
     rRNA            <1..166
                     /locus_tag="KPK72_003362"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            294..410
                     /gene="rrf"
                     /locus_tag="KPK72_003363"
     rRNA            294..410
                     /gene="rrf"
                     /locus_tag="KPK72_003363"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            537..1046
                     /locus_tag="KPK72_003364"
     CDS             537..1046
                     /locus_tag="KPK72_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG2490547.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            1058..1939
                     /locus_tag="KPK72_003365"
     CDS             1058..1939
                     /locus_tag="KPK72_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2490548.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    2067..2272
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2398..3669
                     /gene="serS"
                     /locus_tag="KPK72_003366"
     CDS             2398..3669
                     /gene="serS"
                     /locus_tag="KPK72_003366"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2490549.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3990..4445
                     /locus_tag="KPK72_003367"
     CDS             3990..4445
                     /locus_tag="KPK72_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG2490550.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4446..4537
                     /locus_tag="KPK72_003368"
     tRNA            4446..4537
                     /locus_tag="KPK72_003368"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4480..4482,aa:Ser,seq:gga)
     gene            4575..4839
                     /gene="ffs"
                     /locus_tag="KPK72_003369"
     ncRNA           4575..4839
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPK72_003369"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4916..6553
                     /gene="dnaX"
                     /locus_tag="KPK72_003370"
     CDS             4916..6553
                     /gene="dnaX"
                     /locus_tag="KPK72_003370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG2490551.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6612..6962
                     /locus_tag="KPK72_003371"
     CDS             6612..6962
                     /locus_tag="KPK72_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG2490552.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6975..7574
                     /gene="recR"
                     /locus_tag="KPK72_003372"
     CDS             6975..7574
                     /gene="recR"
                     /locus_tag="KPK72_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG2490553.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7715..7936
                     /locus_tag="KPK72_003373"
     CDS             7715..7936
                     /locus_tag="KPK72_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490554.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         3215 a          825 c         1483 g         2440 t
ORIGIN      
        1 cggaatggat aagcgctgaa agcatctaag cgcgaagcca acttcaagat aagatttccc
       61 accgcaaggg taagacccca gaaagactat ctggttgata ggtcgaaggt gtaagtgcag
      121 caatgtattt agcttatcga tactaatagg tcgaggactt gaccaatatt tatttgatgt
      181 tcattcatta agatatatgt gtagttttta gagtgctaac tctaaagaaa ctagtattac
      241 tagattctat aaacttatta aaaacttaat agaaatatta agcataaaga ttatgtggtt
      301 acaatagcag agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg
      361 ctgatggtac ttggtgggaa actgcctggg agagtaggac gtagccacgt agtctttttt
      421 tatttattat aaaagttaag attaaataga atataaagtc aatattgatt tataataaga
      481 taaaatataa aattatatag tataggtaaa gtttttattt ggaaagaagg tgtaatatga
      541 attctaatga aagaagaagt aaattaatag atattttaaa agaatctaag catcctgtaa
      601 aaggtggaac actagctgaa ctattaaatg taagtaggca agtaatagtt caagatatag
      661 cccttataag agctagagga tttgaaataa tagctacacc tcaaggatat ataatatata
      721 atcaaccgta ttttacaaaa caaataaagt gtaaaaatca taaggattct aaagaaattt
      781 atgatgaatt aaagataatt gtggatttag gtggtattat aaaagatgtc attgtaaatc
      841 atccaacata tggtcagata actgcagaac ttaatattta ttctaaaatg gatatagaca
      901 actttatgaa gaaagttgag acaaatgaat ttaaacaatt atcggttctg acagaatgta
      961 gccatattca tacaattgag gcaataaaag aagaaactat agaagctata gtaaaagagt
     1021 taaaggacaa cggaatatta tcataaggag gagttgaatg aattttttat cattggctaa
     1081 gactgcctca acaaatagca atataactaa gagaacttta gaaaatatgc tcgataattt
     1141 tagtgataat ataccaaata ttatatatgc aataattgta tttgtgatag gtatttatat
     1201 atctaaaata gtaagaagga tggtttcaaa gtttctaatc aaatatggaa tgagtaaagg
     1261 tgtaaataat tttattgtat atggaatata tatatctatg ttatctatta tatcattgat
     1321 aagcttaggt attataggga tacaaactac atcagttgta gctgtgttag gagctgctgg
     1381 atttagtata ggtttagctt ttaaagaaat attatcaaat ttaggctctg gtatgattat
     1441 tttatttttt aagccattta atataggtga ttatattcaa ggttcaggtg tagaaggtac
     1501 agtttctgac atacaaatat ttagcactgt tcttaagacg ccagataata aaactataat
     1561 tatacctaat tttcaactga caagtaataa tataattaat tacacacatc aaaacaaaag
     1621 aagaatagat ttttcatata atatttcata tgatagtgat atagatgtag ttaagtctat
     1681 attaaatgaa atttttacaa acgaaaaaag aatattaaat gatccaaagc ctataatagg
     1741 tttaaactct ataggaaata acaccatgca aatagttgct agaccttggg taaaaactga
     1801 tgattactgg gatgtatact ttgatgtaat ggaaaaggta aaaaataaat ttgatgagaa
     1861 tgatataaaa gttccatttg ttccttcttc attattattt aataatacag gtaatctaaa
     1921 tacaatggat aaaagataag gtcttaatta aatagaaaaa atgaatttaa tataaaaaat
     1981 taaagaaaat tctaaaaaaa tttgaataat agtattgaaa gatgttaata tatataatat
     2041 aatgattatc aaataaaaca attagttaaa aagctgtgaa ggaaaatagt aaaaaacaga
     2101 ttgtattcag agaatgagtg gatggtgaaa actcattaca atggtttttg aatctactct
     2161 agagcttcta gttaaagcta gacgtttacc tacgttatag gtctgagagg attatacatt
     2221 ttagtatagt caattagggt ggcaacgcgg ataaaagatt ttcgtccctt ttatagggta
     2281 aacggaggtc tttttttatt tttaaaattt gtttaaacta agagtttaca agtataatgt
     2341 ttgtaaatta agttggaatt ataacttata ttatcaaaat tattaggagg aattaatatg
     2401 ttagatatta aaagaataag agaaaatcta gatgatatca aaaaagctat ggaaagaaga
     2461 ggtgaaagag aatttgacct tgatgctgta gtggaattag ataataaaag aagagaaata
     2521 cttcaagaag ttgaagttat gaaaaatgag ttaaatgtaa attctaaaaa aatacctcaa
     2581 ttaataaaag aaggaaaaga tgtaacagaa gaaaaggcaa gattgaaaga gttatcagat
     2641 aagataaaag gtattgatga gaaagtaaaa gaagtagaag caaaaatgga atatacatta
     2701 atgagaatac caaatgttcc tcatccagag gtaccacaag gtgaaacaga tgaagacaat
     2761 gttcaaataa gaacttgggg agaacctact aaatttgatt ttgagcataa agcacattgg
     2821 gatataggta caggtctagg tattttagat tttgaaagag cagggaaaat aactgggtca
     2881 aggtttactt tatatagggg attaggagct agattagaaa gatctttaat gaatttcttt
     2941 ttaaatactc atacagctaa gcatggctat acggaagtct tacctccatt tatggccaat
     3001 agaaatagtt ttatagggac tggtcaactt cctaaatttg aagaagatat gtttaaaata
     3061 gaaggattag agtatttctt aataccaaca gctgaagttc ctgttactaa tatacatgct
     3121 aatgaaatat tagatgttgc agagcttcca attaaatact gtgcatatac tccttgtttt
     3181 agatctgaag caggttcagc aggaagagat acaagaggtt tagttagaca acatcaattt
     3241 aataaagttg aattagttaa atttgttaaa ccagaagatt cttataatga attagaatca
     3301 ttaactcatg atgctgaaac tatgttacaa atgttaggtc ttccatatag agttgtaaaa
     3361 atatgtacag gagatttggg atttacagct gcatttaaat atgatttaga agtttggatg
     3421 ccaagttaca atagatatgt agaaatatct tcttgttcaa attttgaaga tttccaagca
     3481 agaagagcag gaataagatt taaaagagat aaaaaatcaa aagcagaata tgttcataca
     3541 ttaaatggtt ctggattagc gataggaaga tgtttggctg ctatattaga aaattatcag
     3601 caggcagatg gttctgtagt agttccagaa gaattaagac cttatatggg tgttgatgtt
     3661 ataaaataaa tttatattaa ttgtatatgt agagtatgct ttgatgaaga gaatatatta
     3721 tgagtaaaca ataaataaaa aaactatgaa attattataa atattaattt catagttttt
     3781 ttattttaat ttatacagca gaattttcat cataaaacac taaatatttt tataaaacac
     3841 tcttgtatga atatctaaaa attattttag aactaattta aaaaaaatat agctatatat
     3901 agtaataatt aaataaacaa aataaaatta agttgaatta taaaatgaat tatgataaga
     3961 taagtttgtt ataagttttg ggaggaatta tggaaagttc attttatatg aaagaagcat
     4021 taaaagaagc atataaagca tataataaaa aagaaacacc aataggtgct attatagtta
     4081 aagataatca aataatagct agagctcata accttacaga aacgttaaaa gactctacgg
     4141 cacatgcaga aattttagcg ataaaacaag catcagaaaa attaggtggt tggaggttga
     4201 ctgattgtga tttatatgtt acaatggaac cttgcataat gtgtagtggc gcaatagtta
     4261 attctagaat aaaaaagctt ataataggga caagacatgt aaaaaattca tatatagaaa
     4321 aacagcatga gtttaaatta gattatttta ataataataa tgtaaaagta gcttttgatg
     4381 ttttgcaaga agaatgctcg atcatattgc aagaattttt taaggcattg agaaaaagag
     4441 attaaggaga gatggtcgag tggtcgaagg cgctcgcctg gaaagcgagt atatgggtaa
     4501 aactgtatcg tgggttcaaa tcccactctc tccgccagaa acaatgaaag taattaaata
     4561 tggttgtaat tttgctgtac tagatgggga ggtagcggtg ccctgtaacc tgcaatccgc
     4621 tatagcagga ttgaattcca tctaaaggct tattttctgt agggccgccc taaataagtg
     4681 gtgttgatgc ttaggtcctg cgcaatgaaa gctcatgaac cccgtcagat ccggaaggaa
     4741 gcagcggtaa gtgatcactt ttgtgtgccg tgggggtgcc taagttgagc taactgtttt
     4801 ggtaacgcct atggaattaa gtcgatagat ggtgtacagc atttaatata taaaagacta
     4861 atcccttaga taatgaggga tttttttatt aattttgcta aagagaggta aaaagatgca
     4921 taaagcatta tatagagcat atagaccaca aaaatttgaa gatgttattg gtcaagatca
     4981 tattattaag actttaaaga atcaaatata tagtgataat ataggacatg cttatttgtt
     5041 ttgtggaaca agaggtactg gtaaaacatc tactgcaaag attttttcaa gagcagtaaa
     5101 ctgtttgaat aaaataaatg aagaaccgtg taatgagtgt gagatatgcg aatctgtatt
     5161 aaaagataat acaatggatg ttgtagaaat agatgcagct tcaaataata gtgtagatga
     5221 tataagagag cttagagaaa gtgtgaaata ttctccagcc aatgctaaat ataaagtata
     5281 tataatagat gaagttcata tgttgtctca aggagctttt aatgcattat taaagacctt
     5341 agaggaacct ccatcatatg taatttttat attagcaact acagagcctc acaaaatacc
     5401 tgcaaccatt ttatcaagat gccaaagata cgattttaaa agagttacag ttaaagatat
     5461 gacccttaga atgaaaaaga tttgtgaaga tgaaggaatt gatatagatg ataaagctct
     5521 taatttaata gctagaaatt ctcaaggagc tcttagagat gcacttagta tattagacca
     5581 atgtatgtct tttggagaaa gtaagataga ttataaagat gttgttgaat taatgggaag
     5641 cgtaaatata gaacaattat ttgaattgtc tcaatgtatt gtagagcagg acactaaaaa
     5701 gtctcttgag atattaaatg aatttgtctt atggggcaaa gatataagaa atcttataaa
     5761 tgacttaatt gatcatttta gaaatttaat ggtatgtaag gtttcatcag aattggatga
     5821 aataatttca ttaccagaag aaactataga acaacttaaa attcagtcta aaaatataga
     5881 tataaatgat ttaataagga ttttaaatat attatcaata actcaggatg atataaagtc
     5941 atcttcaaat ccaagggttt tagtggaaat aaccataatg aaaattgctc aaccaatgtt
     6001 tgatgaaagt aaagaagctc ttataaaaag agttgagaac ttagaaaaaa tgattgaact
     6061 ggggaacttt aagtctgaaa aaattgggaa taataaagaa aaagagtatg aggtcgatag
     6121 agaaatagat gtaaaacaag aaaatgtagt ttatgaagat gtaaaaaatg aggacgttat
     6181 acttatagag tcatcttgga agaatatact taaacaaatt aaaaaagata aaaaaatgcc
     6241 aatatacgca ctcttaagtg aagtaaaaag ttttaatgta tattctaata tgttgtatgt
     6301 tatatttgat gataaatttg attttgcaaa aactagactg agctctcaag atacaataaa
     6361 ttatttagaa aaaacaataa gagatgtttt aaatagaagt tttaatgtaa aaatagtact
     6421 gacatcagaa gttaaagata taaatcttga agttaaagag aaaaaggaca ttggtgagga
     6481 aatattaaag aacatagtga gtgaagagat attagaaata aaagatagta ttgatgaaaa
     6541 tgagagtaaa taataaaata ctattatgat ataataataa aatgaaaata agtttaggag
     6601 gaatagtgtt tatggctaaa aaaggttttg gaggcggaat gatgcctggt ggtggaaata
     6661 tgaacaactt gttaaagcag gctcaaaaaa tgcaagaaaa tatgcaaaaa gctcaacaag
     6721 agttagaaag taaagaagta gaagcctcag ttggtggagg agcagttact gtaaaagtta
     6781 atggtaaaaa agaagttata gatataacta tcaagccaga agtagttgat ccagatgata
     6841 tagaaatgct acaagattta gttttaagtg cagtaaatca agcattgaga aatattgatg
     6901 atatacaagc aagtcaaatg agtaaggtaa ctggaggtat gaatatacct gggttattct
     6961 agtaggtggt atttatgcaa gtttatacag gtcccataac gagacttata gaggaatttt
     7021 caaagcttcc tggagtagga agaaagactg ctcaaagatt ggcttttcat attattaata
     7081 tgaatactaa tgatgtggaa gctttatcta aagcgattat agatgcaaaa agagaaataa
     7141 gatattgctc aatttgttgt aatataacag atacagaccc atgtagcatg tgttcaaata
     7201 aaagtagaga ttcaagtgtt atatgtgttg tggaagatcc aagagatgta gcagctatgg
     7261 agagaacgag agagtttaaa ggacagtatc atgtattaaa tggagtaatt tctccaatgg
     7321 atggaattgg accagatatg ttaaaaataa aggaacttat ccaaagactt ggcaatcagg
     7381 aagtcaaaga aataataatg gcaacaaatc ctactattga aggtgaagct acagccatgt
     7441 atattgctag gttagtaaaa ccaatgggaa tcaaagtcac tcggatagct catggtcttc
     7501 ctgttggagg cgatttagag tatgcagatg aggtcacaat atcaaaagca ctagaaggca
     7561 gaagagaaat ataaaaatcg aggttaatac ctcgattttt gtagtttttt gagttaaatt
     7621 caaattaatt ttgatttaga tttattgaat aaatttcttc tgtattgtat aatttgttgt
     7681 ataaatcatt taaaaatttt gataggggtg caacatgaaa ccaattatga acttagaaga
     7741 acataatgca atgaaagaaa aaagagttaa aaaaatgaaa tcaaaagata aacctaaata
     7801 tgatgatttt gaggatataa gtttttctaa taagaaaaaa actaaaaaaa atgtacgaaa
     7861 taaaaatgct gaatttagaa ataattataa aaattatcag tatgattatg aagaagactt
     7921 ttatgaacca atttaatatt aaataattag aaaataaaaa atc
//
LOCUS       DAEMHZ010000161         7763 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000161 DAEMHZ010000000
VERSION     DAEMHZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.162"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..141
                     /locus_tag="KPK72_003374"
     CDS             <1..141
                     /locus_tag="KPK72_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2490555.1"
                     /translation="TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPI
                     KS"
     gene            153..293
                     /locus_tag="KPK72_003375"
     CDS             153..293
                     /locus_tag="KPK72_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2490556.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            352..933
                     /locus_tag="KPK72_003376"
                     /pseudo
     CDS             352..933
                     /locus_tag="KPK72_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
     gene            1190..1564
                     /locus_tag="KPK72_003377"
     CDS             1190..1564
                     /locus_tag="KPK72_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490557.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            1647..3356
                     /locus_tag="KPK72_003378"
     CDS             1647..3356
                     /locus_tag="KPK72_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490558.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPNNEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            3371..4243
                     /locus_tag="KPK72_003379"
     CDS             3371..4243
                     /locus_tag="KPK72_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2490559.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4244..5032
                     /locus_tag="KPK72_003380"
     CDS             4244..5032
                     /locus_tag="KPK72_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490560.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(5128..5961)
                     /locus_tag="KPK72_003381"
     CDS             complement(5128..5961)
                     /locus_tag="KPK72_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG2490561.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(6029..6658)
                     /locus_tag="KPK72_003382"
     CDS             complement(6029..6658)
                     /locus_tag="KPK72_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG2490562.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            6969..7628
                     /gene="cprR"
                     /locus_tag="KPK72_003383"
     CDS             6969..7628
                     /gene="cprR"
                     /locus_tag="KPK72_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG2490563.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         3056 a          767 c         1160 g         2780 t
ORIGIN      
        1 actagtaagg atgatgataa gatacatgga ataggacttg ctagtattaa atatatagta
       61 aataaatatg gtggtgaaac aatagttaat tactcagata atgagtttat attaaaaata
      121 atgataccaa taaaaagtta ggagagtaca aaatgaaaaa aatagcgtta aacttactta
      181 aaaacatctc agcattaagt tttgggatag ctgtattatc agcaaacagt gcaagttctt
      241 gggtagcaca tcaggcaaaa gagccacaag ctttacaaaa actaaaaaaa taacattaat
      301 aaaaggacct cttcttatta aaggagaggt tttacatgtt gaggaggaaa tatgtttaaa
      361 agattatcat ataaatttgc caatatatta gttaataatg agattgtaga aagtgaagat
      421 tttgaaatat atagatatgg atttgaaact ttgatttatt tcatagttaa tattttggta
      481 gcattattta taggaattat ttttgataga tttatacata cagttatatt tttaagttgt
      541 tattgcactt taagacagtt cacaggaggt tatcatgcta ggaattataa agagtgtaca
      601 ttaacatttg cagttattta tttgataact attttttcag ctaataatat agatataaat
      661 aaatataagt atttattagt attattaatg attattagta tactaacaat ttacaagtta
      721 gctcctttat aacatagaaa caaacctctt agtgaaagtg agaaaaagca ttatagaaag
      781 actgtgcaaa aaatactatt cgtaataata tgtttaatta ttttatgtaa aatacttaat
      841 atttttcagc aatatgtgat ttatgcaata atttctatat attggattgc tatattaata
      901 tatattggaa tgaaggttaa tagcgatcag taagattata aaacattttc taaattaatt
      961 aacttcacta attaatatta actttaaaaa aataatgtca aggtaagtat ttgaacttat
     1021 taaatatagt tgaatctatt gtgggttgta tcaaaggaca aatagattca cttaataacc
     1081 agtttgtaac agagtattga attgtaaaag ttaatacgat agtatgaaaa tgtaatttga
     1141 aattacattt ttataaaaat tatattaact aaggggaggg atacatacaa tgaatttgaa
     1201 gaaaaaaatt aaaaagttag gagttacatt tgcaatagga actataattg tatcaagttt
     1261 agggacatct gtatttgcag atgaggttag cacatatgct ttaatatcta aagctttttc
     1321 aggtaaaaac aatattatat ctagttcagt atacacttct ggaagtagaa cctttaaaac
     1381 gacagattct caagggggat tttcttgtag gctaacagaa agattctcag gctctagccc
     1441 agatagaatt gtaaaaagac atgaagcaaa tgcttcttca aaagttagtt tatcaggagg
     1501 aacatactat ataaggtatg aaggttcaac gaataatgct aaaacatatg caactttaaa
     1561 ttagtattaa aagcatccta taattatttt tataggatgt ttttgtaatt gttataatca
     1621 aaagactggc taggaggggc ataaatatga gtagtattaa ggttatatca gactttataa
     1681 ccaaaataat tagaaaaaat attttatttg caataacaat tattattata aattaccatc
     1741 tcatacaacg atggacacat atatccaata gaggaatttc atttaatgat tacttatcct
     1801 tgagtgtata tacttgtatg acaattagta ttttaaccat gttaaatgta attttttata
     1861 tgagaaaaag ttatgatact gtaaaaatat tatgtagaaa taattttgat tattttttag
     1921 gtatttctgt tgctgtaact agaaacaact ttatattttg ttgcatacca attatatatg
     1981 ctatagcata ctcaagttta ataagaacat tgtcaattgt aacgattaaa tcaactatat
     2041 ttatattgat atttgaatgg tttttaccac ttactatttt atctatagtg atatcattta
     2101 tttcagttat tataaataat ttaatactaa agattacctt atcatcagta tttttatatt
     2161 taacatcaac taatatgttg gatagagtta ttgatattaa agaaccattt tttaaagcat
     2221 tatttaaatc acttaacata tttgaagatc aatcatatgc ttactattct gattttgtag
     2281 ggaaagttaa taattcagca tatgttatag ataagattat acctacacta tttgcaatta
     2341 ctttaataat agtagcatat ctgatattaa atgatattag aaagaaacag attaaatcat
     2401 taatagttat agtagtgtac agtattttat ttattggatt aaattatata tcaatagata
     2461 tgtcaacata tagagatgag gaacgtagtt atggagagta tttaaagaaa tctaatatta
     2521 gtgatattaa aataaagtcg catgatatga agattgactt taaaagcaag tcaaatataa
     2581 aagatagaat tgttttatta aacacatcaa atgaagagtt aacaaatatt aatatgattt
     2641 tagatgaaat atttgatata aaatatataa aagtaaattc ggaaaatata aaatttaaca
     2701 aaaaaaagga tttaattgaa gttaatttaa aggaaagttt agagcctaat aacgaggtga
     2761 cattagatat agagtatgaa ggctatatta atgtgttgac atcttggaat caaaataaat
     2821 atatagctac taaagatgat attatgcttc caccaggttc tttagcgtgg tacccaaaag
     2881 taaatcaatc aagttgtatg gcttataaaa taaatttaag ttctaaaaat aatgtctatt
     2941 caaatttacc tttactgaat aagactagta attttttaaa aaaagattat atatttgaag
     3001 gaaagtcaaa tgatattgca atatatgcag gtgagtttaa agaagaggta aaagatgata
     3061 taaaaataat ctatccatca gatagtaatg taaatatgga tatagaggaa aaaattaaat
     3121 ttttaattga aacaagaaat gaaatattag gtgatagcaa agcatctgta gatgctaaaa
     3181 aatatgtaga tgaagtagat aatgttatgg aatcaataaa aagaaaaaaa attaatatga
     3241 tattagttgc tgacttacct agcaatgaat ttttatatga agactcaaag ggagagaaaa
     3301 ttagcataac tatttatcat tggctttcag gaaatatcct tataatagat aaataataaa
     3361 ggaggttaat atgataaaca agttagaaat aagaaatcta aattttaatt acaaaaataa
     3421 aaaagctctg gataatataa atttaaccct aacagatgga gtagttgcat tattaggtcc
     3481 taatggtgct ggaaaatcaa cattaatgag gttattagtg actctttatg aaacttcaat
     3541 tggagagata gaattaaata atataaagta tagcaaaaac aatgagaaaa tcaaggcaaa
     3601 tgtaggatat gtacctcaag attttgatat gtataataat ataaatgggc aagaatactt
     3661 agaatttgta gctaaaatga gaggtgtatc taagaatgat ttaaagaaac atatacaaaa
     3721 agtagtatca aaagttaatc tggacaagtt tataaataaa aaaataggta catactcagg
     3781 tggtgtaaag agacgactag gtatagcaca ggctttaata ggtgattcta agttaatagt
     3841 tatggacgaa cctacagttg gtcttgatcc agagcaaagg aatgaattta gaagattgtt
     3901 accagaaata agtaaaaatt ctatagtatt aatttcaact cacatagtag aagacataca
     3961 atttaactgt gacaaattaa ttatattaaa tcaaggcaaa atattatatg atggtacaat
     4021 taataagttt attgatatgg ttgaggtgta ttcggtagta gtttctaatg atgaatttga
     4081 atatttagaa cgtaatattg atattattga ttataggaga gttaaagatg gtgtgcaagt
     4141 aaagtatata ggtaaaaata cagagaaact taaagatagc ttaaggatag atacaacttt
     4201 acaagatgct tatataaact ttttacataa ggagttaaaa taaatgtgta tattggagtt
     4261 aataaaattt gagcttaaaa aaatagagct taaacatgtg ttaatatttg taattatttc
     4321 tatattatgc atatttatta caggcatatt agattataat gagttaatat tttataatga
     4381 tgtacctata atggatacat attctatagc aggatggtta ggaattataa agatatcaga
     4441 tgtttttata ccaataatgt cagcaatcac aataatttat gtgttttcaa aagattattc
     4501 gggtaatata aatgagatac ttactttata taataaaaaa aaatataatc aatttatagt
     4561 atttagatgg tgtgttatat tagctatata tactgtatta ttattaattt caattacaat
     4621 agtatataca tttacgtcag tgaaaggttt taacatagac aaaatagatt ttgtttatag
     4681 tgtaaagcca atagatgtgt ttttaaagac gtttcctaca ttattatggt atacaacatt
     4741 tccactaatg atattaacat taacaaaaaa tagatttaca acaatagcaa tattaattac
     4801 atatacattt gctgatatat tttttatttt acatatgtac ccatttgtta gtatgattaa
     4861 tgtaaatgga ttttatattc aaaagatgtt ttatatgcct aacaatatag atgttagatt
     4921 taatgaatta aagaattatt tctttataaa tagaggttct ctagtagtta tttctataat
     4981 aatagttttt tatatagcaa gaaaatcaac ggttttaaaa aagagtaaat agtttaatat
     5041 attttttata ttgttatatt tatatagaat agtgacttta aaaaatttaa agccactatt
     5101 ttttaatatg ttttatctat ataactatta tttttctagg ttttttaata tttcgtaata
     5161 gtgtgttgtg gctatttcac ttgactcgcc ttttttacaa aaagataatt tagatagttt
     5221 tggaactaca cgttcaacga caattttatt acctatcatt ttagattcaa gtatcttcaa
     5281 tgcttctaaa aatctattat ccttttttgc acgattataa aatgataaaa cgtagacata
     5341 aaggaatagg ttataattac ggaaaggata ttcaacttgc ataaataatg tgccaattcc
     5401 atagtgacaa gggccgattg gttttttaat agtccaatgg tcaagtagaa aatctacagc
     5461 tttgtcaagt tttgattctt tatttagata attactaaaa cggaatgcat ttaatatatt
     5521 aagagttggt agtggatttg agtattcagt ttctggacct cgaccaaaac taaatttatt
     5581 gcaacgccac ccaccatcag tatactgtgt atctaaaaaa tgttgaaacg ttttttgcat
     5641 tctaatgtca gatgcatatc ccatatgaca gagtacgtta gcagcatttg cagtatgaca
     5701 agggtaaata cctccatttg gatatagttt gaaacgtcca tcttctcgcc atgtgctaaa
     5761 tatcaagtca gcacattctt taagtaaagt atcatcaggg ttcacaccta actctaatag
     5821 ataaagtaca ctatcaagag tagagaaagg agaacctttt attaaacgct tatcaggtgt
     5881 agtccaatag tctgcaccat tatcatacct gtgtgataat attatttcaa tatctgataa
     5941 agattgattt tctacattca ttttttaccc catttcatat tatattctat taaatctatc
     6001 aaaaacttta ttataatatt atttatattt ataattgatt tattttattt aaaattgcat
     6061 ttatagctgt atcaggaaca actgctaaat cttctgcatt gaagtaataa actatattag
     6121 aatcaaataa tatatttgaa tttggaggaa attcatcatc tccaaaccaa agtataaata
     6181 tcatagatgt attacccata tatgtaaacc tataagctaa atctcccatg tttacttttt
     6241 cagctccaat tttctccata actaacttat atttgtctat ttgagatgta aaaacttctg
     6301 aaagcctata tagagtcctg tttaaaaaat ttggataata aacattgcct cctggtattt
     6361 ctttaaaagt tacatattta ttagttctag gaataccttt agcattgatt aaatatctaa
     6421 ctatcaagat tttaagctca aagttattgc aaaggctacc attactattc caaacttctc
     6481 cacttggata gtctataata tattgtctat caaaaaaatt gagtgtgaat ctatttaaat
     6541 cattattgaa aggtatatta cttaaattac taattgtatg agggtctaat tctttgagtt
     6601 tttttcgaac atagtcaaat ggaacattat aattattttt tgttatcttt tcatccataa
     6661 ttttttcctc caatttaaac aattaaaagt atatttataa aaagttttct tattattaat
     6721 tttattatat attcaaaata ttacaataat aaattgttaa ataaattttt atatttaagt
     6781 aaatacgtgt aaaatgaata taaagttttt gaaatataaa gtttttgtat ctaaaatatt
     6841 ttatttgtaa aatagtaatt aattattgta agctataata tgagagtatt atggttaatg
     6901 tgaattttaa agatattatt aaatatataa attagaatgc tattagatgg ataaatggag
     6961 gagttattat gtctaagata ttagtagtag atgatgagtt agatatgtta aagttgattg
     7021 aaaatgtgtt gaaaagagat ggacatgaag taaaggtaat tagtgatgta gaaaaggcta
     7081 tgaacatgga ttttaattat tttaatctaa taatattaga tgttatgatg cctaagatgg
     7141 atggttttga agtatgtaaa actataagaa ataaggttga ttgtccaata ctgtttttga
     7201 cagcaaaaaa tatggaaagt gatattatgt acggtcttgg tataggtgca gatgactata
     7261 taaccaaacc ttttggtgta ggagaattaa gggctaggat aactgctcat ttaaggcgag
     7321 aaagtagaga aaaaaagaat ttattaacta tctcaaatgt tcaatttaac tttttaggca
     7381 aagaagtttc aattgatggt aaaaaaatta attttactaa aagtgaatat ctaatatgtg
     7441 aatttctagc aaaaaataag ggacaagtat tttcaaaaga aagaatatat gagtcggtat
     7501 atggttttga tggagaaagt gatataagta ctatcacaga gcatataaaa aatatacgaa
     7561 gcaaattaaa acaatatgat atttatacaa tagaaactgt atgggggata gggtataaat
     7621 gggtgtaata agtataagtg ttgtttagtg aaaatattat atatatctac aaaatgtttg
     7681 taaaatatta atctatatat taaaatttaa tttatagatt aatatttttt gtaaatgaca
     7741 aaattgtaag gtattttgtt ata
//
LOCUS       DAEMHZ010000162         7743 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000162 DAEMHZ010000000
VERSION     DAEMHZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.163"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..1488
                     /locus_tag="KPK72_003384"
     CDS             187..1488
                     /locus_tag="KPK72_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2490564.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1564..1839
                     /locus_tag="KPK72_003385"
     CDS             1564..1839
                     /locus_tag="KPK72_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2490565.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3337..3567
                     /locus_tag="KPK72_003386"
     CDS             3337..3567
                     /locus_tag="KPK72_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2490566.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3560..4141
                     /locus_tag="KPK72_003387"
     CDS             3560..4141
                     /locus_tag="KPK72_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2490567.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4720..5397)
                     /locus_tag="KPK72_003388"
     CDS             complement(4720..5397)
                     /locus_tag="KPK72_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2490568.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5586..6950)
                     /locus_tag="KPK72_003389"
     CDS             complement(5586..6950)
                     /locus_tag="KPK72_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2490569.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMLTALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYNRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7078..7599
                     /locus_tag="KPK72_003390"
     CDS             7078..7599
                     /locus_tag="KPK72_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2490570.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3022 a          875 c         1342 g         2504 t
ORIGIN      
        1 ctattatgta gtaaattata taaaacaatg tatcagaata aatagtatat taataatttg
       61 tagtatatca tgacgaataa tacagtcata acaaatagtg agtcataaca aataatgtat
      121 ttataattga taatgtattt aaaaatcaaa atataaaaac ttataaggaa agagagatta
      181 atttatatgg gtaaatcaaa agatgttgaa atagagaagt ctattttagg cacaattctt
      241 ttagacaata aattaagcta taagctagat gaattaaatg aaaacatgtt tatgaatgat
      301 atatgtcttg agatatttaa aattatgaaa gaacttaaaa aagaaaacat agtgattgat
      361 gtggccactg tgaaatcaaa gatagataga aaatctctag caataaaaac aagtgatgtg
      421 actaatctaa ttacttgggg tcagaatttt ggtcttgatg gtcatattaa aattttaaaa
      481 gaaaatttag ctagaagaag cataaatcaa aattgccaaa accttttaca cagcctaaac
      541 cttggagaaa atatcgatac ttgtatttac aaatttgaaa gtaatataaa ggagattttg
      601 gacaaggata cttatgaaaa tgatgatgta aatagtatag cagggaaggt acttgatttt
      661 ttagaaaaca aaaaagatat tggatttaaa tttggtataa aattacttga taccactata
      721 ggaggtcttt ttaaaggaga gctaaccaca atagcagcta aatcgggggt tgggaagact
      781 gctttggcac ttcaaataat gttaaattca tttaagcaag gtaaaaaaac tttatttata
      841 agtagagaaa tgaccagtga gcaagtattt atgagaaata tatgcagagt aactggtgta
      901 agtacaagga atatgaaatc taaagaaata gatgaaaatg attggaaact tattgtaaat
      961 gctataggtg atttaagcga aaataactta atttatatca atgataagat agatacaatt
     1021 agtgcaatta gaaagaggat aaggcaggta aagccagatt tattaatagt ggattatgtg
     1081 caactcctta catctcagaa aagcatggat aagagagaaa gggaggtagc tactttttcg
     1141 agagaactta aaaatatgac tttagatttt aatatacctg taatccaatt aagtcagtta
     1201 aatgatgaaa tgaaagattc aagaccttat ggagatagac ctatgaggga tagcaaagcc
     1261 atctaccacg atagcaacaa tgtggtttat atacatcaat taaaggggag tgattatgaa
     1321 gaagcagtga gagatattgg agaaagtgag gaggcagtta gagcatcaga atacagggga
     1381 attaagatgg ttgatttaat agttgcgaag tgtagagatg gacagactag gcataagcat
     1441 ttttgttact ttggggataa gttacatttt caagaactaa attattaggt caataaagta
     1501 gtaataacaa gcttattaaa agtcgggaaa tagcaaatag attataaaat atgaggtgaa
     1561 aatatgttgg aattaagaaa aattatagaa ttacttgaag aacacaagat agaagtaagt
     1621 attccaaagg agttacataa gtatagttta ttggaatacc ttgaattgtg tcaacaaagt
     1681 gggggaacat atttttatat taaaacactg gaaggggctt taaaagaggc tagaaatgag
     1741 cttatgatta aattgtttaa tgggaaaaat tatatggaat tagcgaggat gtttgaaatg
     1801 agtgttatta atgtcaggca tattgttaga aaaaaataga tatttttgga gaatacaagt
     1861 gtgatatact tctgaaatag aagttattat agttgaaata aaattatggt taataaatta
     1921 aagttgtgta taagtttaag attatagagg agtataagtg tgattaacaa catgattttg
     1981 tgaataaaat tgaggatata tatgagttaa atgtgtgatt aataaggtaa ggttgtgtat
     2041 aagattatga gtaattaaaa ggaggggcta taattaatag tgtagagttg tgtgtttgct
     2101 taagattata taataattta atagattaaa ctatactaat aaggtaaaat cgtgtgttta
     2161 gttaaagctt attataaaat cttgatatgt gattaatgat gcaatatgtg aataaagtta
     2221 agattataat agatttaacc tgtggctaat agaataaaat tgtgaataaa gttaagatta
     2281 taataggttt aatctgtgac taatagagta agagtgtaaa tagagataaa cttcaatatg
     2341 agcttaacct atgattaata gagtaagagt gtaaatagag ataaacttca atatgagctt
     2401 aacctatgat taatagagta agagtgtaaa tagagataaa cttcaatatg ggtttaactt
     2461 gtgattaata gagtagggtg taactaaaga taaacttcaa tatgagttta acttgtgatt
     2521 aatagagtaa ggtgcaacta aagataaact tcaatatgag cttaacctat gattaataga
     2581 gtaagagtgt aaatagagat aaacttcaat atgggcttaa cttgtggtta atagagtaag
     2641 agtgtaaata gagataaact tcaatatggg tttaacttgt ggttaataga gtaagaatgt
     2701 aaatagagat aaacttcaat atgagcttaa cttgtgatta atagagtaag aatgtaaata
     2761 gagataaact tcaatatggg cttaacttgt ggttaataga gtaagagtgt aactaaagat
     2821 aaacttcaat atgagcttaa cctatgatta atagaataag aatgtaaata gagataaact
     2881 tcaatatgag tttaacttgt ggctaataga gtaagagtgt aaatagagat aaacttcaat
     2941 atgggtttaa cctgtggtta atagagtagg gtgtaactaa agataaactt caatatgagt
     3001 ttaacttgtg gctaatagaa taagattgtg aataaagtta tagttttaga tgggtattaa
     3061 cctgttacta atagaatatg attaggtgta aaattataga gctgtataag aaaatattat
     3121 agatatatta aatattagat aataaatatt tatatagata cacaaaagaa aaggcgatat
     3181 gaagctaaac ttaatgactt catatcgttt tttatttgaa actcctttgt atttagagtg
     3241 tgatttttaa tattgaggtt ttatgtgatt aagtcacaga taaaaagtaa gtatcttgat
     3301 ataattctta tagattaata atatagggag gttattatgt ctaataaaaa aaagaagtat
     3361 gcaaatattt tagcaaatga gtgcgttgct tgtggtagct gtataaaagc atgcccaaga
     3421 agtgctatct cagttccatg tggcattagt gcaaaaatta atagagattt atgtgttgga
     3481 tgtgggattt gtgaaaagat atgtccagca tctgtaattg agattataac aatattaaaa
     3541 gaggaagaag gtaagtgtca tgaataataa aaaacattgg tatgactatt tatggatttt
     3601 ttcttcaata tatttgatat tgggatttgt aaatattatt ttcgcttgga ttgggctaat
     3661 ttgtttcttt gttccactag ctatttctat tgttaaggga aataaagctt actgtaataa
     3721 gtattgtgga agaggacaac tgtttaattt gcttggaaat aaattgaagc tgtcaagaaa
     3781 tagagatatt cctaaattta ttagaagtaa gtggtttaga tatgggtttt tgacattttt
     3841 tatggtaatg tttgctaata tgcttttttc aacttattta gtgtttgaag gaagtagaaa
     3901 ttttaaagaa gtagtgactt tgttgtggac tataaaaatg ccttggcaat gggcgtatca
     3961 tggaacttta gtttcacctt gggtggtaca gtttgcattt ggtttttata gtgttatgct
     4021 tacatcaact gttcttggtt tgattactat gatattgttt aaacctcgtt catggtgtgt
     4081 ttattgccct atgggtacta tgactcagat gatatgtaag gcaaagagta agtctgtata
     4141 gaaaggcctt atgtgatatt tacagttttg ttcaagtaga gatgagtagt aggtttgaat
     4201 atatatgaca aatatatgag tataggggca tagatttagt ttgtgtaagg taaactgatt
     4261 tatgcttctt ttttatgtct tagattattg gctaagtgga aacaatgaat ttagatatta
     4321 agaattattc agttatttga agaaaataga ataataatta acattatatt aatggatatt
     4381 cattacatat aagccagtta atagcaagat ttatttaata tgaaataggt caagtattta
     4441 aactaaataa ggtcatgaca attaatataa gtatagatat aaaaagatta gaagttataa
     4501 cttataaaag gtattctata taaatagtat ggggaaaata gtcaaattaa cttattagaa
     4561 acaaaaaata tacataaaaa ataaatgtaa atatcaaata agatactata tcaaaataga
     4621 tataagcatc ttttttatta ttgaaattat gaaaatatat ttgattttac acaaaaaaga
     4681 gtgcctcatg aactaaaaag ttcattttga gacacacttt tacagtgtca aagaacgaag
     4741 tttatcctta tccaatactt ttataccacc acgagataaa gacacaatac cttctctagc
     4801 aaaatatttc aacattctcg atacaacttc tctggcacta cccatatact tagcaatttg
     4861 ttcatgagtc atatttatag tgctactgcc atttttagct atctcatcaa ttaaaaaaga
     4921 tgccaaacgc ttatcaaaac tcataaaaag aatttgttgc atagcccaca taacatcaga
     4981 aaaacgttcg gtagctaatt tatatgaaaa taactctgca tgaatatttt ctgtagataa
     5041 ttttgcaaaa acagaggaac taatctgtat aatatcgcag tccgtttcag catctacata
     5101 gacatcaaaa gttattgttt ttagaataca ggaagctgat aaaatacata catctccatc
     5161 atcaagtcgg aacagtgtaa cctctcgacc ttcatctgaa agtatgtatg tacgcactgt
     5221 tccagacttt ataatcaaaa taccaataca gtcactatca ccacaatgta ctggattccc
     5281 ttttttgtaa tgcgaggtat ttgctgaaga aattaataat tctttttgta agtcagtaag
     5341 tttatcccaa aaaggcaagc tatgtgtaag tatatcaatt tcattttgtg aaatcataca
     5401 atatctcctt tcttaaattg ttataatccc atgatacaag tgaatagata aattgacaag
     5461 tagtttgtta agaggattat ttagtcagat agtaatagta tgatttatgt agaaaaaata
     5521 tgataatata tattcttgct attaatgaga aagtaatatg ttttgatgat agtaggtttg
     5581 tataactaat gcataccgtt tatttttaaa agattaagtt ccttatgtaa ccttatagac
     5641 ataaaaccag caattatagc agccatcaaa tcagctattg gttgtgcata taagatacca
     5701 tttaacccat agaatacagg aataatcaaa attataggga taaaacagac accttgtcta
     5761 cacataccta aaaatccacc ttcctttgct tttcctagtg cgaggaataa tgtagaatac
     5821 actgtataga atccaaatag cataaatgaa aacccatttg ccctaagagc tttactacca
     5881 ttttctatta ataaaatgtc accacttgta aattgtgaaa ttatagattt tgggaagagt
     5941 atcaatataa gaccaagcac tacacaaaat attgtagtcc ataatatgga agtttttatt
     6001 gctatatgta atctattgta gttctttgaa ccaaaattat aaccagcaat tggttggaaa
     6061 ccttttataa aaccaaatac aacaagacta cctaaagatg ttattcgtgt tacagcaccc
     6121 attccagcta tcacagaatc accatacttt gtagcctcca tattagttaa tgtaatggca
     6181 agactagtca ataattgaaa tattaaggta ggtatgccaa tttttaggat ttctgaaaaa
     6241 atttcttttg aaaaacagca ttcgttaatt ctaaaactaa atatactttt cttgcttaaa
     6301 atatatccta cataaacaag tgttgaagca atctgtgaga tagctgttgc aatagaagca
     6361 ccccttaccc ccagaccaaa agtataaatg aatatcgggt ctaaaatcat attaagcaaa
     6421 gcacctaata acatagcaca catagttgtt ttagcagctc cttcacttgt gacaatatta
     6481 gtcatagtga cattgaatat attaaaaata gatgacacga cataaatgct agcataagat
     6541 attgcataag gcataatact ttttgtagct cctaacaatt ttaaaattgg attgagaaaa
     6601 atcataatca caataataac taatgtaccc actataatac tactgtaaat agatgtactt
     6661 accacttttc ctgctttttc attatcacca tggcctaaaa gtctggcaat atatgaagca
     6721 gctccatttc caaacaataa tcctaatccc acaacaagct gacctaatgg aaatgcaacg
     6781 gatatagctc ccatttgact tgttccaagt ccacccacaa agtatgcatc aaccaaattg
     6841 tataatgcag ttagcatcat acctatcatg gtaggtaatc ccatagataa caatgctttt
     6901 gagattgggg cagaccctaa taattcaatt cttttgttac tttctttcat acttaacact
     6961 cctcttctat tgacattaag tctaaattat aatactataa attatagtaa gtatggaaaa
     7021 tagtactata atttatagta tttgtcaata gtaagaaaac taaaggagat gatttttatg
     7081 gctacaatag atttaattgt attgggaata gtaaagcaac aacccaagag tgcttatgat
     7141 attcaaaagg cagtggaata caggaatata tcacgttggg ttaaaataag tacaccatca
     7201 atttataaaa aagtaattca attagaacaa aagggatata ttgaaagtaa aactgtgaag
     7261 aatggaaaaa tgccagaaaa agcagtttat tcattaactg aattaggtga gacacatttt
     7321 ttagatttaa tgtttgatac atctagcaaa ccaataaata ttcttttaga ttttaatgct
     7381 gttatagtaa atttaaatag ccttacacca gaaaaacaga aattatgtct caataatatt
     7441 gaagagaatg ttcaagagtt aaagaaaact attgaggaaa atataagtat aaagacagaa
     7501 gtaccagaga cagcaaaagc agtgttggag caacagttgg tgctagcaca agcattggaa
     7561 acctggataa attctttaaa agataatatg ggaaattaga attagagaag ctaacttaat
     7621 aatcattaaa caaaaaccag catatttaat aatgagatat tagaaaaaat aaaacaaatg
     7681 atataaactg aaaagtatat ttaaaataaa gctcacttaa ttgagggaga atgttaaact
     7741 ttt
//
LOCUS       DAEMHZ010000163         7709 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000163 DAEMHZ010000000
VERSION     DAEMHZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.164"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..587)
                     /locus_tag="KPK72_003391"
     CDS             complement(<1..587)
                     /locus_tag="KPK72_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG2490571.1"
                     /translation="MARVEELLSAQELINYTKTRKLKETMGDLLFPKQKIEGLEIKMI
                     KGASNLPVSASVHAFNTEAEIASREGANLSIAELALIKRKIKLDEKDIIVLEKPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLSTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTS"
     gene            complement(599..979)
                     /locus_tag="KPK72_003392"
     CDS             complement(599..979)
                     /locus_tag="KPK72_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490572.1"
                     /translation="MYFKEVNFNNTPEFLASQHYINFTKTALDTDIVADENGKKYVLA
                     GSLLGKSGKVVKVTRGGSSGSYTYSLSEDPIGIVFSTVDVTYGPQPVASMVEGYVITE
                     RLQGEYVKEAIDTIKAKLPNIKFM"
     gene            complement(995..1567)
                     /locus_tag="KPK72_003393"
     CDS             complement(995..1567)
                     /locus_tag="KPK72_003393"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2490573.1"
                     /translation="MKKDELIALGLSEEDAKKVEAESLKELDNYINKIEHEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQNKLKEKEQREFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDNWATQKSFLIKTGETKTNYTPDNGN
                     ENTLSRAENIAKEKNDEGSKNPYADAWSIK"
     gene            complement(1620..1892)
                     /locus_tag="KPK72_003394"
     CDS             complement(1620..1892)
                     /locus_tag="KPK72_003394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490574.1"
                     /translation="MFKKRYIMKPSKISVRNIIAFIITVIGIALGVFIGINIIMDHVL
                     GIANMVDNNTFTCVRLVYGLVGVISGYLIGKAIYLIASLISCIIYE"
     gene            complement(2102..3124)
                     /locus_tag="KPK72_003395"
                     /pseudo
     CDS             complement(2102..3124)
                     /locus_tag="KPK72_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(3114..4517)
                     /locus_tag="KPK72_003396"
     CDS             complement(3114..4517)
                     /locus_tag="KPK72_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2490575.1"
                     /translation="MELDVIKKLIEQTNSKHNNFVKKADEAEKYYKNQNDIIRDRSPN
                     NVGKVNTANNPLRNADNRIPFNWFGFLVNQKVSYLFTYPPTFDVGDDGINSKITDILG
                     DRYPKEAKTLGKNASICSKAWLHVWEDDNNDFQYANIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDEGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NVYKHNFGEVPFIEFPNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRMICRHLEYSPKR
                     VLQTWTRNMIQNDLELAEICSKSVGIVSEKTNLKNHPLVDNAEEEEKQLKKEKVDSQQ
                     EYDDLIPNNQDGVIDET"
     gene            complement(4522..5790)
                     /locus_tag="KPK72_003397"
     CDS             complement(4522..5790)
                     /locus_tag="KPK72_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2490576.1"
                     /translation="MVILMITRVQFNPSFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVNGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMKM
                     REEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIITIANSKGLEKSLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSIKAQIDYLKQLRIHVHPSCINTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGVRILTPNGRR"
     gene            complement(5768..6460)
                     /locus_tag="KPK72_003398"
     CDS             complement(5768..6460)
                     /locus_tag="KPK72_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490577.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSKG
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETQDLINEIKNKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVRELNRTDDEDDEL
                     KIVIDYGDSNDN"
     gene            complement(6526..7110)
                     /locus_tag="KPK72_003399"
     CDS             complement(6526..7110)
                     /locus_tag="KPK72_003399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490578.1"
                     /translation="MNRLNYLNMRIKMFISSYLPLYLILLTIYSDKINSFDKIKLIIR
                     FEDKIVSLFIIAVFILVIISFRTLIDLRRTKGNENHKFEFFNKTEDTIISYMMTYIVP
                     ILSTDFLSTKTMTINLILYSLIGLMYIKLNLIYLNPLWLLFGYSVYKSDNEVVIITNI
                     PYGRLKTLRNTNLKSSYLGNDIYLIQRSENDNIN"
     gene            complement(7133..>7709)
                     /locus_tag="KPK72_003400"
     CDS             complement(7133..>7709)
                     /locus_tag="KPK72_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HBG2490579.1"
                     /translation="KFEENNQEKEINFFRRVTKFKKLSSKGFMGFIKNNRFKKLDSNL
                     LGLDGFIDIAIYGQDVLIFNHISLERIFSIADQYLEKAQNTIDIIRDINRIDNFEQFE
                     EDCLNDRRITRTLTKMLSEEARLENCFDNFANVVNAIDLFQLDINIDRNGERDKVIYE
                     NKEQLMDIIRLVRDSYYKSIIHDRKGTDDSI"
BASE COUNT         2266 a         1409 c          806 g         3228 t
ORIGIN      
        1 gaagttaatg accttgttgg agtaaatcca gtatcaacaa ctattttatc agtcatatta
       61 taaatatctc ctaatatatc tggtgttcca ctagaccaag tttttgtttc tttatgattc
      121 gttggagttc catattttaa agaagcttta actccatttt catttatatt caattctcca
      181 gttgataaaa cttccattct cattgcttct attctagtat ttacacttga tacaaggtta
      241 tcaacatcat taaatatttg gtttatcatt tgagtttctt cttgagaatt tcttggtttt
      301 tcaagtacaa ttatatcttt ttcatctagt tttatttttc tttttataag tgcaagttca
      361 gctatactta aatttgcacc ttctcttgat gcaatttctg cttctgtatt aaaagcatga
      421 acacttgctg atactggaag attagacgca ccttttatca tctttatttc aagtccctca
      481 atcttttgtt ttggaaataa taaatctccc attgtttctt tcaactttct agtttttgtg
      541 tagtttatca actcttgagc tgacaataat tcctctactc ttgccatatt tcatcctcct
      601 acataaattt aatatttggt aattttgcct ttatagtgtc tatagcttct tttacatact
      661 caccttgtaa tctttcagtt attacataac cttctaccat tgatgcaact ggttgtggtc
      721 cataagtaac atctacagtt gagaacacta ttcctatagg gtcttctgat aatgagtatg
      781 tataactacc cgaagaacct cctctagtta cctttactac ttttccactt ttacctaata
      841 agcttcctgc taacacatat tttttaccat tttcatcagc cactatatct gtgtctaatg
      901 ctgtctttgt aaagttaata taatgttggg aagctagaaa ctcaggtgtg ttattaaaat
      961 ttacctcttt aaaatacata ctttttcctc ctttttattt tatactccat gcatcagcat
     1021 atggattttt agaaccttca tcattttttt ctttagcaat attttcagcc ctgcttaatg
     1081 tattttcatt gccattgtca ggagtgtaat ttgtcttagt ctctcctgtt tttattaaga
     1141 aagacttctg agttgcccaa ttatcagtct gttcctttag ccctgtaaaa gtaccatttt
     1201 catattttat tttttctaaa tcaagttctg cttttgctgc tttagtgcta tgaacattaa
     1261 gtttaataag ttcattttct aacgccatat caaactctct ttgttctttt tcctttaatt
     1321 tattttggta ttcttcatcc tttgcagtta atttagtctc atatcctttt ttaagctctt
     1381 caatttgctc ttttgtcatt ccatctttaa aaccttcaat tgcttctttg gatgctttta
     1441 attcttcttt taccttttca tgctcaatct tattaatgta attgtctaat tcttttagtg
     1501 actcagcttc taccttcttt gcatcttctt cactaagacc taatgcaatt aattcatctt
     1561 ttttcatttt atttaatatc tcctttcatt tcttacaaaa taaaaaatct ttgaacaaat
     1621 tattcataaa taatacagct tattagtgaa gctataaggt atattgcttt tcctattaaa
     1681 tatccactta taactcctac taagccataa actaatctaa cacaggtaaa agtattatta
     1741 tctaccatgt tagctattcc taaaacatga tccatgatta tgttgatacc tataaaaaca
     1801 cctaaagcta taccaataac tgtaataata aaagctataa tatttctaac acttatttta
     1861 cttggcttca taatatatct ctttttaaac atatttccat cccccaaaac ttatttaatg
     1921 ccttctctta gacgttcctc taattgtttt acaatactat ctatatcaac tttcatttta
     1981 aataaataaa gccctgtaca aaacacaatt attgataaaa catataattt aagtatttca
     2041 aatcaccctc tcaataaatt tttacataat aaaatcacct actaatttag taagtgtctc
     2101 taatgtattt cttataccat tcattatact tcatactaga tggtacataa tatgtttttc
     2161 catctactcc ttttgatgct ctgtaacctt cttcatcact aaaaaaagga gctgttgttg
     2221 tcctgcaacg acaatgaaat ggtggagctg ttattccaac ttgataatct ttcatatcaa
     2281 atacttttcc atctaactct ctacatatat ttgaagttct taaatctagt gtggcaataa
     2341 tctcatattt ctctacatct aaatccttaa aacaatcttt tctagttgct gatgcaaaga
     2401 aagctgattc agtcattatt aggttcttag cttgagattt agatacatta aacatcttag
     2461 caaagtcatt tactagattt tttggatttt cacctctaat aattgattga gttaacttag
     2521 tgtgtagttc atttactaaa gcaggtctat gcttgcccca aattctttca ctaaagttca
     2581 atccatcact agtccatggt tttgagataa ctttatttat tctattagta tcaagtaaca
     2641 ttaaactcca accaacattt actccttgtt gaatactaaa agctgtatga taatatccac
     2701 ttgtataaat atctctcatt agcttatcaa tactatcaag ttcattccca tataaaacct
     2761 ccacttgttg ttgtatttgt aactttaaag cttcaagtct tgttatatga actctagcac
     2821 tagcattttc taactctttc atccactttt gatttatagc attttcttta ccatgtctga
     2881 tatattcctc cacactccac ttaaattctt ctagttctct tgtatttaac agtttcttag
     2941 cttctaataa agatattcct tcattttcag caaatctgtt ataccatgat aatatatctt
     3001 tttctatact attcatagct aatttgtatt gtttttccaa ttcaagataa tattttacac
     3061 ttttgttatt ctgagattct tctaattgtt caaatctctt cctccaataa tctttatgtt
     3121 tcatctataa caccatcttg attattagga attaaatcat catattcttg ttggctatct
     3181 accttttctt ttttaagttg tttttcttct tcctctgcat tatctacaag tgggtggttt
     3241 tttaaattag tcttttctga tactattcca acactcttag aacatatctc agctagttct
     3301 aaatcatttt gaatcatatt cctagtccaa gtttgtaaaa ctcttttagg cgaatattct
     3361 agatgtctac atatcattct tactagttta gcgaacccta atctaaactc agtttccatt
     3421 aaacctgctt ttaactctaa taaagtgtat agaaatttaa gtgctacacc acttgtatta
     3481 gcaaaatttt cgggtttagg gtcaacacct tgaccttgta catatatttg cttttcagtt
     3541 gttttaagaa gcgaatctct agcttctatt ggaatattta ttgtaattgt acttagtcca
     3601 ctcttatcat ctgctccgct actttgtaaa tcaatagttt tatattcttt aagtcctttt
     3661 aagaactctg ttaagtctgc ccctccataa tttgtaagaa caaaaataac ctcttgtata
     3721 tcttcaatgt cattcacaaa gccactataa actttgtcat atacatcaat tagatgttta
     3781 acattatcta aatctcttac ctctaaatca ttgtttggaa attcaataaa tgggacttct
     3841 ccaaaattat gtttgtacac attagtttga gtttcaagtt tattatctaa gtttttctct
     3901 ataaatttat taagtatctc taagccatta atattactat ttccatcttt attttgataa
     3961 gtataacaac attcatctgt ccaatactca tagattacat attcttttcc ttcatcatca
     4021 gtctttttat attctctaag tactgcaaga agctttctgt ttaaatctga tgaatatact
     4081 gctcttattt ggcgagggtc tatattagca tattggaagt cattgttatc atcctcccaa
     4141 acatgtaacc atgctttaga gcatatacta gcatttttcc caagtgtttt ggcttctttt
     4201 gggtatctat cacctaaaat atcagttatc tttgaattta taccatcatc tccaacatca
     4261 aaagtaggcg gataagtaaa cagatatgat actttttggt taactaaaaa accaaaccaa
     4321 ttaaatggta ttctattgtc tgcatttctt agtggattat tggctgtatt tactttgcca
     4381 acattattag ggcttctatc tcttataatg tcattttgat ttttatagta tttttcagct
     4441 tcatcagcct ttttaacaaa gttattatgt ttactattag tttgttcaat taactttttt
     4501 attacatcta gttccaattt ctcaccttct tccatttgga gtaagtattc taaccccatt
     4561 atttttaagt ttattatcta tagaatatct aagagcagcc attgcatcat ccataaattc
     4621 aactggttca tcaagatata atccagttct ttcgtcttgt ttccatttcc attgttgtat
     4681 ttcttttatt gtatttatac aactaggatg tacatgtatt cttaactgtt ttaaataatc
     4741 tatttgagct ttaatacttc ctggtccttt tttaactcct ttagctttat atcctgcact
     4801 tttccacatc ttaattctat cgggttcagc gctatcacag tacataaata gactcttttc
     4861 taaaccttta ctatttgcaa tcgttattat ttctgaggta tccatttcat gtacatatat
     4921 ttcattacat atatataact ctccatcctt aaagccaatt ctaagtattg catttgcatg
     4981 attaaaacca aagtcttgtg ataacctcat attatcaaag tattcaaatt ctgtaggaaa
     5041 ttcatgtata acatagtttt taagtattgc tccaccagtt tctccccatt ctccaagtcc
     5101 atagacttta tacccttctg ggtcttgttc ctctctcatt ttcattcttc tataatacgc
     5161 ttcatcaata aatctatttt gtagataagt actatgatga gtaaatatat catcattttt
     5221 atagtcaaaa tactttcttt ttatccaatg agtagctgat actgggttaa atgtaaatgt
     5281 catttgatag tataagttag gattagttaa aatacctctt aaacggtcgt ctagtatgtc
     5341 tatgtcactt tccataagtt ctgtagcttc ttcacaccat acccatgtta attttccttt
     5401 tgagaagtta attgatttca atttttctct ttgttttgca tcattaacac ctctaaatat
     5461 tatagagtta ccattaactt tactcttaat ttctaaagga tttaaagtag ttttccaata
     5521 cttatcagct tgtttaccat aaatacgatt tatagctcct gtaagctctg cataagttga
     5581 atatttgtgt gtagcttctg attttctaac tactaataag ttagctcctt gatacttttt
     5641 atctcctaac tttagtatat agtcctgtgc tacattaaca gattttccac tacctgctga
     5701 acctttcatt gctctatatc tttttttagt aaaattagct tctttaaaag atggattaaa
     5761 ttgtactcta gttatcatta gaatcaccat aatctattac tatttttaat tcgtcatctt
     5821 catcatcagt tctattaagt tctcttactt cacattttaa cttttcaact ctatttttct
     5881 gctcctctgt agccaaattc caatccttat gaatcatttc atcatactgt ttaattaaac
     5941 ttctaagttc actcatagcc ctactctgtg cattaagaaa agatgcttgc ctatcccatg
     6001 caaattggaa ttcatattct atcttttcac cattttctgt actttcatgt tttttgagtt
     6061 ccttaatcat ctcttccttg tctttaacat acattatctt ttgtgctctt attattgctg
     6121 catattgaat tgttatctgc tcccaaagaa tatcgaattt atctttattc tttatctcat
     6181 taattaggtc ttgagtttct tcgggtaagt attttgagaa gaaaccaaac ttttcagcat
     6241 tcttattctc ttttggagca ccatatccaa ctgaattttt attagaaaag ggtgcacccc
     6301 ttttatttat aggtgcaccc ttcttttttc cctttgacca gttatatctt tttatccatg
     6361 actttaaagt atttaaacta atgtcatact ttgctgatat ttccttttgt ttcattcctt
     6421 ttaagtaatc ttgttttacc ttttctttga catcttgcac atcaccacct ctttatttgt
     6481 ttgttttggg aataaaaaag aactctggtt agagtcctgt ttttgttagt ttatattgtc
     6541 attttcactt ctttgtatca aataaatatc attacctaaa tatgatgatt ttagatttgt
     6601 attccttaga gtctttaatc taccataagg tatattggtt atgatgacaa cttcattatc
     6661 agatttgtaa actgaatatc caaacaataa ccacaaagga tttaaatata taagattcaa
     6721 tttaatatac attaaaccga ttaaagaata taaaattaaa tttattgtca ttgtttttgt
     6781 actaagaaaa tctgtagata aaattggaac aatataagtc atcatatagc taattattgt
     6841 atcctctgtc ttattaaaaa actcaaattt atgattttca tttccttttg tccttcttaa
     6901 atcaattaaa gttctaaacg agatgattac aaggataaat accgctatta taaataaaga
     6961 aactatttta tcttcaaatc ttattattaa cttaatctta tcaaaactat taattttatc
     7021 agaatatata gtaagtaata ttaagtataa aggcaagtat gatgatataa acattttaat
     7081 tctcatgttt aaatagttca acctattcat gcaaccacct ccttttattt aattatatac
     7141 tatcatcagt tccttttcta tcatggatta tactcttata ataagaatct cttactaatc
     7201 ttattatgtc cattaattgt tctttatttt cataaatcac tttatctctt tctccatttc
     7261 tatctatatt tatatctaat tggaataagt caattgcatt aacgacatta gcaaaattat
     7321 caaagcaatt ttctagtcgt gcctcttcac ttaacatttt agtcaaagtt cttgtaattc
     7381 ttctatcatt taaacaatct tcttcaaatt gttcaaagtt atctatcctg ttaatgtctc
     7441 ttattatatc tatagtgttt tgagcctttt ctaaatattg atctgctata gagaaaatcc
     7501 tctctaatga aatatgatta aaaattaaaa catcctgacc atatatagct atatctataa
     7561 aaccatctaa tcctaataaa ttagaatcta attttttaaa cctattattt tttataaaac
     7621 ccataaaacc tttacttgat aattttttaa atttcgttac tcttctaaaa aaatttattt
     7681 ctttttcttg attattttct tcaaatttt
//
LOCUS       DAEMHZ010000164         7662 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000164 DAEMHZ010000000
VERSION     DAEMHZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.165"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..950)
                     /locus_tag="KPK72_003401"
     CDS             complement(<1..950)
                     /locus_tag="KPK72_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2490580.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKL
                     KDIEEGYFYLLEDVYFDMKDVLEDLMLYRMNEYEYKYEIK"
     gene            complement(989..1849)
                     /locus_tag="KPK72_003402"
     CDS             complement(989..1849)
                     /locus_tag="KPK72_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2490581.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2118..2327
                     /locus_tag="KPK72_003403"
     CDS             2118..2327
                     /locus_tag="KPK72_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2490582.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2327..2593
                     /locus_tag="KPK72_003404"
     CDS             2327..2593
                     /locus_tag="KPK72_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2490583.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2622..3194
                     /locus_tag="KPK72_003405"
     CDS             2622..3194
                     /locus_tag="KPK72_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2490584.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3429..4619)
                     /locus_tag="KPK72_003406"
     CDS             complement(3429..4619)
                     /locus_tag="KPK72_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2490585.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4879..5310)
                     /gene="dut"
                     /locus_tag="KPK72_003407"
     CDS             complement(4879..5310)
                     /gene="dut"
                     /locus_tag="KPK72_003407"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG2490586.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5423..7651
                     /locus_tag="KPK72_003408"
     CDS             5423..7651
                     /locus_tag="KPK72_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG2490587.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDTNKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNDDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
BASE COUNT         2679 a         1223 c          978 g         2782 t
ORIGIN      
        1 ttttttatct catacttgta ctcatattca ttcattctat aaagcattaa atcttctagt
       61 acatctttca tgtcgaagta cacatcttct aataaataaa aatagccttc ctctatatct
      121 ttcaatttat ctatagtatc cattttttta cttatatctt tcataatttc taaattttgt
      181 atatttacat ctatgattgt taataagtac attctaacag tttttattat ttcaacagaa
      241 aaatctacaa tattataatt tccttctttt ttatataatg actccttaaa tttgttaaaa
      301 caattatcta aattagaatt tatatcgtta aatggtggtc ttattatgtc acgtattatt
      361 aaaagatttt gtatgtctac attatttgtg attgcctcat ccaaaataaa taaattatat
      421 gcatcattta atgggtcatg ggcatctcca ctaaactcta agttaacaaa ttcaagtaaa
      481 tttttcaatg atatatagtt catacatctc ataccacaat ttatatattt ttcttttata
      541 tctacaaata aatctctaat gttatttaca aaccttggat ggttattttt tttagcactt
      601 ctcatatctg tgttattgaa acatgtcaaa tccaaattac caaaagtata tattccttta
      661 atatcagaaa atatgccaag ccatttttta aactttctca tgacctcttc ataacttggt
      721 gcactcttca attcttcaga agatatatgt gttaattctt gtatatgagg atacaactcc
      781 tcattactta ctggtcttat taaagattta aatttatcaa tattttttgt tttttcatca
      841 tacataactg ctccgattgc aattatatca gaattaaata ctgctctttt tgatttagaa
      901 tttggcatat tcatttcaaa atctatatat actcttttca atataaacac cctttccaat
      961 tgtttatagt gattcttttt aactattttt attcaaaatt ttctaagaaa gaatgtttct
     1021 ccccatcttt ttgccaacca agtactgaat caagatttac attttctgcc gccttaaata
     1081 tagatttttc tacattttta aaatttgatt ctaaactctt tattaagtct tttatctcat
     1141 atcttttatt acctgttttt ttagcagcaa ccatacatgc acaatttaat ggccatatac
     1201 cactattttg aataaatctt ataatatgtt cttctcttat ataataaagt ggtcttataa
     1261 tttttatacc ttcaaaatta gtagaattaa gtttaggaag cattgtttta aaatttccag
     1321 cacacaataa gttaagcatt gttgtctcta ttacatcgtc ataatgatga cctaatgcaa
     1381 gtttgttaca ccctaattct tctgcctttg aatataatgc tcctctcctc attcttgcac
     1441 acatatagca tggataatct tttgctattt catctgctat ctcaaatatc cttgaatcaa
     1501 ataaatgtat tggtatattt agatactcac aattatctat aagtaattgt cttatatttg
     1561 catgatatcc agggtccata gcaataaatt caacatcaaa attaacttgt ccatgttttt
     1621 ttaactcttg aaacatttta gccataagaa tactgtcttt tccaccagaa atcgctacag
     1681 ctatcttatc accttcttca accaatttat aatctcttat tgccttcata aacttagacc
     1741 ataaattttt tctatatttt tttattatac ttttttctat atctcttact ggaagtcttt
     1801 cctcaaaagg aactataatt tcacagcctt ttcctgatag ttcactcatc taatcacctc
     1861 gtatatttat gttaataata ttttacaaaa aagtctacct tcatccacaa aatactataa
     1921 tttttacttt aattttacta aataatttat agtttacaat aacacataaa cttataataa
     1981 cataaataac ctctttcttt tattatcttt tttatttttc tcatttaata ttttattatt
     2041 agtaacgatt tttaaatgat tgaatattat acactagata gagttaccta tctatttaca
     2101 acaaggaggt tttcatcttg gaaaataaga ctagagatat caaaaaaaat tgtggtccat
     2161 ctttggctag accatacatt aataatcaaa ttttcacaga aatgtactgc ctttctgatg
     2221 cattaaaatt tggaactata tttccagaac taaacttact agagtctgaa atgtacaaca
     2281 aagaattata tgctaagcca aaaaaatata gaggaggtag aagataatga aagactcatc
     2341 tagagctgaa cttatgagaa aagttcagga aacatctttt gcatgtgttg atatgaactt
     2401 atatttagac aatcaccctg atgataaaaa tgctatcaac acttataatt cattatgtaa
     2461 tcaatttgcg caagctagat atgcttacga aaataaatac ggtcctttaa ctaactttgg
     2521 gtatgctcca agtagatgcc cttggcaatg ggctgaccaa ccttggcctt gggacagaga
     2581 atttaattat tagacatcaa gtattggagg attgaaaatt catgtggata tatcagaaaa
     2641 ctatacaaca cccagttaat ataaaaactt gtgaccctag aatggctaaa tttcttataa
     2701 ctcaatttgg tgggccaaat ggagaacttg ctgcatcttt aagatattta agccaaagat
     2761 atacaatgcc tactggtaat atgcgtgcac ttttaacaga tattggtaca gaagaactag
     2821 ctcacgttga gcttatatgt actatggttt atcagttaac ttctgatgca agcccagaag
     2881 agttaaaagc tgcaggtctt ggttcaaact atgctcaaaa tggatatgga atttatccaa
     2941 cagattcaaa tggtgttcca tttgatgtaa gacctatagc agttatgtca aatcccgtaa
     3001 ccgatttaca tgaggatatg gcagctgaac aaaaagcact tgcaacttat tatcaactta
     3061 taaacctaac agatgacgtt gatgttatag atgtattaaa attcttgggt caaagagaaa
     3121 taattcacta tcaaagattt ggtgaagctt taatggatgc ttacgagtta gaagaatctc
     3181 aaaaaatgtt ctaaaatttt tatttgtata actattatta tttgataatt tataatttac
     3241 tgttgtgtta aaatttataa aaaatataaa tataatttat tttctataaa cagagaaaat
     3301 aaataaaaag caggtatcaa tcaaatagta acatgtatta tactatacat tactatcaat
     3361 attgtacctg ctttcatatt ataagcaata aagaaaaatc tatttattag gttttataat
     3421 aaaaacaatt ataaaactct tcttgcattt acatatctac ttgaatagta gctactattt
     3481 atattagata ttgttacttt ttttgatgaa cctgaagatg catgtatcat atctccattt
     3541 cctatgtata ttccaacatg acttacagaa ccattatttg aaccttgtgt atcaaagaat
     3601 actaaatctc cacttctaag gtctcctcta tttacatatg ttccatactt actttggtct
     3661 ctagatactc ttggtatact aactcctaca gattttttca taacgtactg tgtgaatcca
     3721 gaacagtcaa aactatttgg accttctgct ccccatacat atggttttcc aagtagtgtc
     3781 tttgcaaaac ttattacact atcagctcct tgacttggag aattagtcga ataagatgga
     3841 attgaagtat caacagaacc tttttcagca aggtattgac ttgacacata tccagtttga
     3901 ttgccataac ttacttttgt ccatcctcca ctttcagaaa tacttccaac ctcagtacca
     3961 taacttaatg tagctatttt agaatgactt gtacctggtc ctgttctaac gttcaatgac
     4021 tttgctgtta caacctttgt accctttaca aggtcatttg aactgttttc agcattttca
     4081 tttgtagagt catttacact agttcctaaa tatttacttg aaacataacc tactctacca
     4141 ttagaactta tttttgacca tccatttgac tcagatatgt atccgacttc agtcccatat
     4201 cctagtgttg atattttaga actacttgta cttggtcctg ttctaaaatt taatcctttt
     4261 gctgttacaa cttttgtcga cttaactgaa gtatcactac tttcattgct acttccaagg
     4321 ctatttattg ctacataatt tccatgcaca taacctgtgt ttccattata tttaactttt
     4381 acccaagaac cttcttttcc tacgtactca actttatcac ctttgtaaag tttccccatt
     4441 gattcatggt ttgtacctgg tccttttcta aaatttaccg cattccctgt tattgttctg
     4501 ctttctatac tcacactctc tacttcagac tcattagcat atgctaaatt ccccatagta
     4561 ggcattatta tcgcagttgc cattatactg gctgctattc tcttttgatt aaaattcata
     4621 actttccact catctcctct tttctactat ctttatttca cttcaaaata taaaagaaat
     4681 aattgacact atcaaaatat caattatttc ttttatatta atattattac aaatttgtaa
     4741 ctatttgtct atagtaatat ttgcttacca taaattacca tgtataaatt ttaatcttta
     4801 atagcctgta atttagtaat atagccatct tttacctttt ttaaaaatgg ctatattttt
     4861 taactttttt gtaacttttt aaaatccagt tgagccaaac ccaccttttc ctctttcgga
     4921 gtcacttaat tcttttactt cttctatgtt aatatcatag ataggtttta ctatcatttg
     4981 agcaattttc atatgctttt caaccttaaa atcattttta ccatgattta ttaatataat
     5041 tttaagctct cctctgtatc cttcgtcaat agttcctggt gtatttaata cagtaacaga
     5101 atgctttaag gcaagcccac ttctaggtct tacctgcgct tctgtcatag taggaagctc
     5161 tatacatatc ccagttttga taagtttagt ctctccagga ggtattacaa cctcttctat
     5221 agaatacaaa tccataccag catcaccttt gtgagcaaaa ttaggtatta tagcatcatc
     5281 atttaatttt tttactttta aattgtacat cttatatctc ctttcaactt tctataaata
     5341 ttataactta aatttcgacc tcttgaagtt ttttattttt ttgtggtaat ataattatta
     5401 gtgttttaaa ggaggatttt ttatgaaact tgtacttgct gagaaaccat ctgtggcaaa
     5461 aactatagca tcatttttag gtgcaaagac cagacaagat ggttactttg aaggaaatga
     5521 ttatataatt acttatgcag ttggacactt agtatcatta tatgatatga aggattatga
     5581 taaagataaa tactctggct cttggaaaat gaacaatttc ccatttgttc ctgaggataa
     5641 gtttaaattt aagattgata gttctaaaac taaacaattc aacactgtaa aaaagctttt
     5701 gaatagaaaa gatgtagaat atgtaataaa tgccactgac aatgaccgag aaggagaact
     5761 tatagcattt ttaatatttt tactggcaaa aaataaaaaa ccagtaaaaa gaatcttggt
     5821 aaatgaatgg acaccagaag atattacaag aggtataaaa aacctaaaag atgaagatga
     5881 aatgagaaat cttcaagctg ctggatatac taggcttatt actgactggt tgattggaat
     5941 aaatttcact tctgttgcta cattgaaata tggaaatggg aaactattaa acataggtag
     6001 ggtaattctt ccaacagtta aattagtcta tgatagagat atggaaatac taaactttgt
     6061 tcctaaaact tattatgaaa tagaggggca ttttaaagct gaagctggtg aatataaagg
     6121 taaatatgtt aagggtaaag aaagtaaatt tgatacactt gaagatacaa ataaaattat
     6181 agcttcaatt acatctgaaa ctggtaaaat actagataaa aaagttacta tgtcaaagga
     6241 atatgcacca aaacttttta gtttgacatc acttcaagga tatataactt ctaagtattc
     6301 caattttaca tctgataagg ttttaaatgt ctgtcaatct ctctatgagg gtaatggtaa
     6361 gggtggatac ataacttacc ctagaactga ttccattttc ttagaagaaa gtttggcttc
     6421 aaaagcttct caaactttag ataaattaaa ggttggtctt gaatatgaaa ataaaattaa
     6481 attttctaaa actaaaagag tttttgattc ttctaaagta gactcccata gtgctataat
     6541 tccaacttat ataattccaa aaagtttatc taaggatgaa caattagttt atgatgccat
     6601 aaaagataga tttgtagcaa actttatgcc tcctgctgaa tatgagaata cagaaattaa
     6661 gactgaagta gataattgta ctttcttaac aaaaggtaag gtgcttaaat caaaaggata
     6721 ccttgaggta tataataagg aagaaaaaaa cgatttactt ccacttgtaa ataaagatga
     6781 tgttgtagat gtgttagaaa taaaaccatt aactaagcaa actactccac ctaaaccata
     6841 tactgaggac acacttttaa aggctatgaa aaattgtggt aaaaatgtgc ctgaagaaga
     6901 tacaacagtg ctatctggat attcgattgg aacatcagcc acacgtgctg atgtgttaaa
     6961 gaagataagt caagtgggct atgtaaagaa aaaaggtaaa tcttattcta taacagacct
     7021 tgggaaaaat ttagttgaaa tttttcctgt taaagactta tttgatgtag attatactgg
     7081 aaaacttgaa aaaagtttaa gcgatattca aaaaggaaag tatacaagaa aagaatatct
     7141 tactaatatc attagtttta tatatcaaaa tgtcaatatt ataaaacaag atgcttcaaa
     7201 aaatataaac acagaagcat atactttcga ttcaaaaact aaaaaattta ctaaaacaaa
     7261 agaaaaaaaa cagaaaaaag atgtaactaa tactgaaaaa gaagttgcta tatcaaaaga
     7321 ttctaataac aaaaaagaag ttaaaaatga tgatgaaaat caatctcttg gcaaatgccc
     7381 catttgtcaa ggtgatgtac tagaatttga caaaggcttt gcttgtaaaa atcataaaga
     7441 atgtaaattt gttatttgga aaaatgataa gtatttaagt ctttataaaa agaaagttaa
     7501 caaaacaatg gtcaaaaata ttttaaaaaa aggtgaaact aaagtaaaat ctttaactgc
     7561 taaaaacggt aataaatttg atgctacatt aaaatatgat aaaaatccag ataccggcta
     7621 tttcaattgg aaaatagagt ttgacaacta aatattcata ag
//
LOCUS       DAEMHZ010000165         7374 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000165 DAEMHZ010000000
VERSION     DAEMHZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.166"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            431..1123
                     /locus_tag="KPK72_003409"
     CDS             431..1123
                     /locus_tag="KPK72_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490588.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1319..3379
                     /locus_tag="KPK72_003410"
     CDS             1319..3379
                     /locus_tag="KPK72_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2490589.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3585..3908)
                     /locus_tag="KPK72_003411"
     CDS             complement(3585..3908)
                     /locus_tag="KPK72_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG2490590.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3901..4602)
                     /locus_tag="KPK72_003412"
     CDS             complement(3901..4602)
                     /locus_tag="KPK72_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG2490591.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4880..6265)
                     /locus_tag="KPK72_003413"
     CDS             complement(4880..6265)
                     /locus_tag="KPK72_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2490592.1"
                     /translation="MPINSFENYPMNWKPKRPSKWQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLQLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6373..>7374)
                     /locus_tag="KPK72_003414"
     CDS             complement(6373..>7374)
                     /locus_tag="KPK72_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2490593.1"
                     /translation="QGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIENFKKEILEI
                     DITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNKELRFIFKH
                     ELIHYKRKDNLFKLLISTICIIYWFNPILYLLKKYFHELCELSCDEIVIDKCTTSEIK
                     EYAYIILDTIKYQKTLKYSSFVSQFLNKKNIILKKRLDSMFNQDKKKINLGLKFLLTF
                     IIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPELIQTIGRG
                     KKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         2835 a          845 c         1042 g         2652 t
ORIGIN      
        1 taaataagta gctattattg cagtgaaatg aatgtttcta agtatatttg ggtggaagaa
       61 tggtggaaga aaattagtga aatgtaattc ttccaccatt cttccaccca aaaaataaaa
      121 actgttcaat aagaattaat atacatcaat tcagctgaat gcaataaaaa tacttaaatg
      181 aaagtaatct gaaaacaaca taattcaaat caatttattc aaataaacct caatttatct
      241 taaatcttaa gaaatattta agaattagga taatattttc ttaagattta tattttatta
      301 tatagttata agggaaagtt acaaaaatgt aagccaaagt aatataacaa tttagtcatt
      361 gttgagaaag ggagtgaaag atattgtata atatagataa gtattattat acaatagagg
      421 aagtgaaaat atgtacaata tattagtagt agacgatgat aaagaaattg ttgattcgat
      481 agaaatatat cttaaatcag aaggatttaa aatatataaa gcatatgatg gaatgaaagc
      541 tttggaaata ataacagaga atgacattca tctaatacta atggatataa tgatgcctaa
      601 attagatgga atcaaagcaa ctgtaaaaat acgagaagaa agaaatattc caataatttt
      661 aatttcagca aaaagtgaag atacagataa aataatagga ttaaatattg gagctgatga
      721 ttatataaca aagccattta atctattaga gcttatagct agggttaaat caaacttaag
      781 acgatatgta actttaggta attatgaaga tacatgtaaa gaaattctaa aaagtggagc
      841 tttggagtta aatacactaa caaaagaagt taaaattgat ggtgaaaaca taaaaatgac
      901 tccaattgag tataaaataa ttgaacttct tttagaaaat aaaggacgag ttttctcaat
      961 agatgagata tacgaaaaag tatggaatga ggaaagtttt aattcagaaa atacagtagc
     1021 agtacatatc agacgaataa gagaaaagat tgaaataaat ccaaaagaac caagattttt
     1081 aaaggtggtg tggggagttg gttataagat tgaaaaattt tagtggaaaa attataaaag
     1141 aaaagagttt aatatttaat tgcattttga ttttagtcat ttttacattt acacaaatgt
     1201 taggaatttt aatatgtttt aattttttct ctgagagttt aatttactct atggggatta
     1261 tgcttttctt aaactttatt ttatataaaa tactgacaat gtagatgagg tgggcaactt
     1321 ggagaaatat agagtattaa agttagtttc attaatcata atatctatct catttccatt
     1381 aactatattg ttggtgacaa taggacttgc tgagcctaaa tacaatatac aaaaaacttc
     1441 tacaaagagt gggatttttg atatatctga tgatttttat gatagtaata tatttgacga
     1501 aggaacaatg gcaagtctat tacccaagtt taagctatct gtaggtggta aattaactag
     1561 taaagaaaaa ggtaaattcc aacaagaagg taaaagtata gatttaagta gtagtgattc
     1621 tgagtatgat aaaatcatga ttaatgaaca agcttctgaa gaatatttaa gttatctaaa
     1681 aaatataaaa gtgtatgtta ttaatacata cacaggagta ttttatacaa atacgtcata
     1741 tgctagttta tatgatttta aacaaaaaac taagagctat tgtaatgtag agataataaa
     1801 taagatagga aagagctcat atattaaaac aataaatgga gaaaaatttg aactgaataa
     1861 tctgagtctg gaactaaatg aagtagatga aggttttgag gcatatgtat cttttccaga
     1921 agagccaact atagaagatg gaatagtata tactaatttt caaatattta aacaagctac
     1981 agaaaaagta agattatttt ttgtagctag tttaagttta gcaattatat gtttattatc
     2041 tataatttta tatatcaaaa taaaaggtga agttttaaaa agaaataatg tatttttact
     2101 aatatacagt aagataccta tggaatttaa tgtaatatta gtatctattt caattgcaat
     2161 tttaactata tatgctcttg ctgtatctta tcatattgat gtaattgcat tagtttcttt
     2221 atctttactg atagtatcaa ataatatatt ttttataaat gtgcaattga gctatttaga
     2281 aaataaaatg tgtatattta agacaagtat tatagtaaga gtgattttat ggttaataaa
     2341 aactgtaagg gaggtcagta acgctataaa gaaagtatct ttagcaagga aagttattgt
     2401 tatagtgata atatctttag ctttaatcat ggtaattagc actcgaagta taggtatggg
     2461 attactgttt tctatatgta tttttttagg atttacaata tatataacta aaagactttc
     2521 atatcttagt tacgtaatag aaggtacaga aagaataaaa aagggagaat tagattataa
     2581 gataaaaatt actgggaatg ataattttac gtctttagct gaaaatataa ataatattgg
     2641 agaaggtctt gatagagcta tagaagagca agtaaaaagt gaaagaatga agtctgacct
     2701 tataacaaat gtatctcatg atttgaaaac tccacttact tctataataa attatgtaga
     2761 tttaattaaa aaagaagaat caatacaacc agagtatata aatgattata taaatgtact
     2821 tgagtcaaag tcaaaaagac taaaattatt gatagaagat ttatttgaag cgagtagagt
     2881 aagtagtggc aatatagaat tacaaattag caagattgat ttagtgcaac tattaagaca
     2941 aagtataggt gaacttgaag aaaaattatc taaaaataat ttatatttaa aattaaatgt
     3001 tccaaatgat aaggtgtata tatgggcaga tggaagaaga atgtatagag tatttgaaaa
     3061 tcttttatct aatatagcaa aatattcctt agaagaaact agagtctata ttgatgtata
     3121 tgactatggt gaaaatgtaa aggtaactat gaaaaatata tcttcttatg aattaaattt
     3181 tgacccaagt gagattatgg aaagatttaa aagagctgat gaatcaagga atactgaagg
     3241 aagtggatta gggcttgcta ttgcaagaga tttagtagct ttacaaggtg gaaaatttta
     3301 tatagaaata gatggagatt tatttaaagc aaatttagag tttcaaaaat atgaagaaga
     3361 aataacaaat atggaatgag atagtattta agtatatata ttttttttac tgtcgaaagc
     3421 ttttattata atatattaga atatttacag tagagaatga agagtaaaaa cagagtaact
     3481 ttaagtcact ctgtttttta tttaaaatca ataatataac aacttaaatt aaaattgtac
     3541 acatagtttt aaaataactt atacaataaa acattatcca aatatcacaa gataattcca
     3601 aataaatata tagatatcac tcctgtaata atagaaatac caatagattt aaacttatat
     3661 gcaacaagaa gtacaatcat aaaagatata attttagggt tagctataga taaatataga
     3721 ctatctccat taaaaaatac atctttagca gttaaggaag taagtattgc tacaggtata
     3781 aatttcatga tttttttcaa aaaatcaggc atctcttttt ttgaaaaaat taacatagga
     3841 agcactcttg gtagatatgt aacaatgtac attccaatta taataaaaaa tatataatta
     3901 ttattcataa gattcctcct ttttacagta agattcttct ataaagaaac caattaaaga
     3961 tgctaaaatt gatgcaacta ttattactaa attgcttttg aataatgcat ataaaataac
     4021 agaaataatg attgatgata taaatactac aatataaatc ttatttttta tttgagaaac
     4081 tattaaagct ataaacatag aagtcaatat gaagccagaa atagaactgt ctatagatat
     4141 actttctcct aaaaatgctc caataaaatt agctaaaact gatgaaatta agcagaagaa
     4201 attaagataa atagcatttc tatcagacca atttccagca gtaaattttt cataattaac
     4261 tgcaaaagtt tcatctgtta tagtatgaca aaatggcaat ataaattttg gacttttatt
     4321 ttttatgtac atgttcaaat tagaactcat tagaaaatgt cttaagttaa ctataaatgt
     4381 agtcaaaaca atagatgtta cacttgcacc agtagaaatc attgaagctg caatgaattg
     4441 agaacttcct ccaaatacta aagttaccat tagtgctatt tgaaaaggtg aaagaccaga
     4501 tttttgtaat agtattcctc ctactatacc tataggtata taacctaaga atataggaat
     4561 tgaagcaata aagctttctt ttaattgttg agttctttcc acaataaatc actccttttg
     4621 tattaaatgt attaaagtat tagtaaaaca gaaaaataga ttaattgtat aattgcaatt
     4681 ataattaaaa ataaatttgg attaatatat aaaatactat acaacaaata gataatctta
     4741 aacggttatt aaattttatc tatttgttat tatgatgtaa taatacatat taatctctat
     4801 aagttaatta ctatctggtg taattttatt ttaacaacaa aaaaaaatac acacaattaa
     4861 aatattgtgt gcaatacaat tattttttct cttctaatag tctttttaat acgctcaaac
     4921 cagctttaag ttcttctgta ctttctgtag cacatacagc tattcttatt gcagaaattg
     4981 gtttttcttt tccaacagca aaacgttcag aagcatagac ctgtacacct tctttaagtg
     5041 cttgtatctc aaatttttct gccattattc catcaggtag aatcaaccat ctaaatatac
     5101 attcttcatt tcccaaaaga ttatatccag ataaatattg attaataatc ttgtttcttc
     5161 ttcttgcaga tttacggcgt gcattaatta atttatcggc ttcacctgat gtaatcatgc
     5221 gataagcaat ttctgttaaa aaataagatt gagatagatt aatgttgtat aatgcgcttg
     5281 ataatgcctc tcgatactgt ttaggtgtag agatatatgc tagctgtaaa ctaggtgcaa
     5341 taatttttga taaactcgta atataaattg tttgattggg cagataggaa gccactggat
     5401 ttaaatgtgt ttcattaagt aaactatgaa tagcatcctc aataacaatt aaattgtatt
     5461 tagatgcaat atttgcaatc atttttcgtc cattttgtga cattatatgt gtagttggat
     5521 tttgataatc tggtattatg tatagacctt taatattttc atttttacaa gcatataata
     5581 gtccttcttc actaatctca ttatcttctt gtttaattgg aattagttga atcccaagca
     5641 ttttagctgc tgtctttatt ccaggataag ttaatgggtc tacacctatt ctatctccat
     5701 gttgaaacaa accagctaaa atagcaacaa ttgcattttg tccaccactt gcaggaagta
     5761 agctatctga tgtagtttca agcccagctt tataaattaa tttggcaata gcttcttttt
     5821 gccatttagc accatctctt tgtccatatt ggaatatggt tttaaaatct atttccttaa
     5881 ttatgtgttt aaacaaagta ttcatttctt ctagcgtgaa atctggattc attgtgccca
     5941 tttcaataaa tgtaatttta ttattactgg acatcaagaa tagatttgaa cgtgtatcat
     6001 aagatacaaa agttccactt cctgtaactc cacttattag accttttttt tcacaaattt
     6061 taaaagctct agagatagta ctcacattaa catctaagaa atctgccaat tcacgttggg
     6121 gtggtaattt agtacctggt aataaaaatc cattgttgat atcttgttct agttgttctg
     6181 caagcgcctt atataatatt tgccattttg aaggtctctt tggtttccag ttcataggat
     6241 aattttcaaa agaattaatt ggcataagtt ttcacctcac attattaatt tgattttttc
     6301 tttatctaaa acaataaatt ctaaagatac aaataaaaac tgataggaaa taattctatc
     6361 agtttttgtc ttttaattga tttcaaatct tcctataatt tttatacctt ctttatcata
     6421 aagagggatt gtttttttaa atatattaaa atttcttttt cttgtatata aaacagcttc
     6481 ttctggtgtc tttggttgat tatcacaatc gtacaaatct ctttttttta cgtagccgac
     6541 ttttttacct ctacctattg tttgtataag ttctggttca atggaatttc catttgagtc
     6601 tattatatgt gtaccatagg tttctccatt tttattataa ttaatcttac caatagtata
     6661 ttctacatct tcattttgtt taaaattgga gttgatagaa aatattgacc ctattattat
     6721 aaaggttaat aaaaacttaa gacctaaatt aattttcttt ttatcttgat taaacataga
     6781 atctaacctc ttttttaaaa taatattttt tttatttaga aactgagata cgaatgatga
     6841 atacttcaaa gttttttgat actttattgt atctaatatt atatatgcat actctttaat
     6901 ttcactcgtg gtacacttgt caatcacaat ttcatcacaa gataattcac ataattcatg
     6961 aaaatatttt tttaataagt ataaaattgg attgaaccaa tatatgatac aaattgtact
     7021 aattagaagc ttaaataagt tatcttttct tttataatga attaattcat gtttaaagat
     7081 gaatcttaac tctttattat tgtacttttt ttctggaatt attattttac agcgtgttaa
     7141 acctataatg gcaggagaat aaataccttt aaccttatat atctgaagtt ttttatttat
     7201 acttagttct tttgcttgtt catctaagat actagtaata tctatttcta aaatttcttt
     7261 tttaaaattt tctatgtaat tataaaattt aaaatttgtt cttaaatagt aaaaagagat
     7321 tgctataatt cctaatatcc agatgtacat aaatatatct aaagtaatgc cctg
//
LOCUS       DAEMHZ010000166         7298 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000166 DAEMHZ010000000
VERSION     DAEMHZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.167"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..602
                     /locus_tag="KPK72_003415"
     CDS             159..602
                     /locus_tag="KPK72_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2490594.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            633..2450
                     /gene="ftsH"
                     /locus_tag="KPK72_003416"
     CDS             633..2450
                     /gene="ftsH"
                     /locus_tag="KPK72_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2490595.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2628..5330
                     /locus_tag="KPK72_003417"
     CDS             2628..5330
                     /locus_tag="KPK72_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG2490596.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALERK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5364..6062
                     /locus_tag="KPK72_003418"
     CDS             5364..6062
                     /locus_tag="KPK72_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490597.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            6419..>7298
                     /locus_tag="KPK72_003419"
     CDS             6419..>7298
                     /locus_tag="KPK72_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG2490598.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYG"
BASE COUNT         2901 a          790 c         1303 g         2304 t
ORIGIN      
        1 agataacaaa atttcaattg aagttacaat attgtaattg acaataaata cttaatacta
       61 taatatttaa tacattaaac attatagtgt taagtattta ttacagtatg cacttcagcc
      121 taaatttgaa ataaagtatt ttcgtaggga gcgtttgtat ggatgagatg agttttaaga
      181 aagagctact agagttgact agagatataa atatgaaatt tacaacttta ttaagtgatt
      241 tttatcagcc acttggtatt acagcagtgc aagctttgat tttatctgag ttatgtgaac
      301 atggagagaa aaaaataagc gatttaggga agaacttaaa catgacaaat agtaatgtat
      361 cagtaatctg tcaaaggctt gaaaaaaatg gctttttaaa tcgcataaga gatatagaag
      421 accaacgaat tgtaaaagta aaagttacaa ataaatcatt ggatatacaa gaacatattt
      481 caagtagtat ttttgatagt tattttgaaa atatgaccag tgaaaaattg caagatatgg
      541 aagatataat agaaggttta gagaaattaa ataaactttt aacttacatt gattgcaaat
      601 aacataaaaa taatttaaag gagaataaaa ttatggatga acataaaccc caaaaaaggt
      661 cagcactcta ttattataca gttataataa tagtagtaat gtttttaaat gctttaatat
      721 ttccaagttt aatggataaa aaaatggttg agattgacta tggaaagttt ctcacaatgt
      781 tggatacagg aaaggttaaa tctgttgaga tagaaaataa tagaatagca ataaatccat
      841 ctagtagcaa ggataagaat atatacataa ctggtagaat ggatgaccca gaattggtag
      901 atagactaaa aaatgcaaaa gtagaattta ctaaggttat tcctaaggaa aattcacctc
      961 ttttaagtat tttacttaca tggataatac ctataggtat tcttatgcta tttggtaact
     1021 taatgatgaa gtctatgcaa aagcgaatgg gtggtaatgc tatgcaattt ggaaaaagca
     1081 acgccaaaat gtatgtatct gctcaaagtg gaaaaagatt taatgatgta gctggtcaag
     1141 atgaggcaaa agaagcttta actgaaatag tagatttctt acacaatcca gaaaagtata
     1201 aaaagatagg agcacaaatg cctaaaggag cacttttagt aggacctcca ggtacaggaa
     1261 aaacactcct tgcaaaagct gtagcaggtg aagcaaatgt accatttttc tcaatatctg
     1321 gttctgagtt tgttgagatg tttgtaggaa tgggagcttc taggattcgt gatttattta
     1381 aacaagcaaa agaaaaagct ccatgtatag tgtttataga tgagattgat actattggta
     1441 aaaagcgtga taatggaagt ggattaggtg gaaatgatga acgtgaacaa actttaaatc
     1501 agttgttaag tgagatggat ggttttgatg gtggaattgg agtagtaata cttgctgcta
     1561 caaatagacc tgacagtctt gacaaagcat tacttcgtcc aggaagattt gatagacgta
     1621 taccagttga acttccagac cttggaggac gtgaagctat attaaaggta catgctcaaa
     1681 atgtaaatgt aagctcagat atagattata atgcaattgc aaggtctaca tcaggagcat
     1741 caggtgcaga acttgcaaat attgtaaacg aagcagcact tttagcagtt aagtctggca
     1801 gagatatagt agaacaacag gattttgatg aatctgttga aactgtaatt gctgggtatc
     1861 aacgtaaagg agctgtaatt tcagataagg aaaagaaaat tatagcatat catgaaatag
     1921 gtcatgcact tgttgctgct atgcaaaaac attctgcacc tgtccataag attactataa
     1981 tacctagaac ttcaggagca ttgggatata ctatgcaagt tgctgaagat gagagtgtac
     2041 ttatgtctaa agaagaagca attgataaaa taactacttt tacaggtggt cgtgctgctg
     2101 aagaggttat ctttaacact tgtacatcag gagcatcaaa tgatatagaa caagctacta
     2161 aaattgcaag agctatggtt actcgccttg gtatgagtga agagtttgat atggtcgctc
     2221 ttgaaactgt aagcaatcaa tatttaggag gagatacttc tcttgcttgt tcttccgaca
     2281 ctgctgctaa aatagatgaa gaggtattta aaatagtaaa ggcttgtcac aaaaatgcaa
     2341 ttgaaatttt agaaaataat attgataaat tgcatgaact tgctaattat ttattagaaa
     2401 aagaaactat tacaggtgat gagtttatgg aaatacttaa cttaaactaa aatgattata
     2461 cacaagataa aaatgcctat tgttaggcat ttttatttta caactaaatt ttttaaattc
     2521 atatttaaat ctttacataa tcttaatcat taaaaataca tcttaactac aattttatat
     2581 cggaattgat attataatag taatgaatgg tgtaaggagg ttttttaatg gatagaccta
     2641 atcctgatat tctattagaa aaaattaaaa atgaagaaga aaaactaagt agaggtcaat
     2701 taaaaatatt ctttggttat gcagctggag taggaaaaac ctactctatg ttggaatctg
     2761 cacaaaattt aaaaaaagtg ggtgtagatg tagtagttgg atatattgaa ccacacacta
     2821 gaccagaaac attggcttta cttgatggct tggaaattct tcctgtaaag gagatagaat
     2881 ataaatctat aaaactaaaa gaatttgatt tagatgctgc tttagaaaga aaaccagaag
     2941 ttatattagt agatgaattt gcccattcta atgttgctgg gttaaggcat acaaaaagat
     3001 ggcaagatat agaagaattg ctgttagcag gcataaatgt ctacactact gtaaatgtac
     3061 aacatttaga aagtctaaat gacattgttg agataattac aaacgtatca gttaaagaga
     3121 ctataccgga taagtttttg gatacaaata cacagttaga acttatagat gttgaacctg
     3181 atgtacttct tgaaaggttc aatgaaggta agatatataa gaaagaacaa gctactagag
     3241 caaaaaataa tttttttata aaagataacc tggttgcact tagagaaatc gcacttagaa
     3301 aaacagcaga gagagttaat aaagaagttc aaatgaccag actatcaaag ggtgatgtaa
     3361 ctgtaatacc tacatcagat acacttcttg catgtataag tccatctcca tcttcagcaa
     3421 aagttattag aactgcatcc agaatatcag acagtacatt tgcaaaatgg atagctttat
     3481 atgttgaaac tccaaacaca gcaagacttc catttgatga acaaaaacaa ctacaaaata
     3541 acctaaaatt agctaagaaa ttaggtggag aaataattgt tttacatggt gaaaatataa
     3601 tagagcaaat acttagaatt gcaaaactga gaaatgtaac aaaaattgta ataggtagaa
     3661 atcattcaag taataaaaga tttagtaaaa gatttaaaaa agatatagtt gacaaattaa
     3721 ttgatgaagt tgattatata gatatacata taattccata taaatttgcc tcagatgtta
     3781 aatacaaacc taaaaaggat aaatcatcaa taaaatctaa atttaaaatt agcaaagttg
     3841 attttataaa acttatattt attactttga tgataagtat tttagcatat gcagtacaaa
     3901 gcatgggatt tataagagaa aatatattgt taatatatat gttgggtgta gttttagttt
     3961 ctatgtggac taaagggtat agtacaggta taataagttc tgtatttaat atagttcttt
     4021 taaattattt tttcacagca ccattatata cattatctat agcagattca aactatatag
     4081 taacactggt tgtattctca atagtaggta taataactag cactcttacg tctaagatac
     4141 agcatgaagc agaaactgct gctaaaagag aagaaaatac aaaaatgata tatcaaataa
     4201 ttaagggatt tttaagatta tcaaataaag atgatatagt aaacaaaggt atagaacttt
     4261 tgtctcttag cttaagtaga gatgtaattt gttatttaaa tgtagataag cataataaat
     4321 caaagcttta caaaaaaaat actaataata aggataaaaa tgatttggat agtgaagatg
     4381 aaaaagctgt tgcaagttgg gttttatcca attcaacagt agcaggaaat gacacggata
     4441 cacttcctgg ttcaaaggga tactatatac caattgtagg aatgaattta actttaggag
     4501 tagtgggaat atcatgtatt gattcaaaat tggatacaga agatataagc ttaattgaaa
     4561 caataattgc tcaaatggca atagcgcttg atagagaaat cttgtcagaa gctaaagaaa
     4621 ataccaatct tgagattgaa agggaaagat taagaagtaa tcttttaaga gcagtttcgc
     4681 atgaccttag aactccacta gcaggaatat ctggtgcagt aagtacaatt ataaaaaaca
     4741 agggtactat aggtcaagat ataatagatg aattacttag tggtgtatat gaagatactc
     4801 agtggttaat aagacttgtt gaaaatttac ttagtatgac caagatagat gaagggaaat
     4861 tggaagttaa aaaacattct gaactggttg aggaaattat atcagaagcg ttacaaaaaa
     4921 taaaaaaaag aattgaaaat gcacttatag atattaatat accagaacag atattatttg
     4981 ttcctatgga tgcaaaatta atagaacaag ttttaattaa tttaatagat aattctttga
     5041 aatattcaaa ggaagattgt aagatagaga taaatgtata tgagaaagat gattatgttt
     5101 ggtttgaagt atctgataat ggtcctggta tatcaaagga acttaaaaag catatctttg
     5161 acagattttt tactggagaa gaaggagcta aagattccag aaagggtgtt ggacttggat
     5221 tatcaatttg taaatctata attcaagctc ataaaggtga aattatggta gaaaacaata
     5281 aagataaggg ttcaactttt aagtttagtt tgcctaagga aaatgaatag ttttaaattt
     5341 taaaattagt tttggaggaa gaaatgagta aaaaacttat attgttagta gaggatgata
     5401 agacaataag aaaatttata tctactgcac ttttaacaca ggattatgat gtcaaggagg
     5461 ctataactgg aaaagaaggt atctctattg ctgtctcata ttctccagat gttgttcttt
     5521 tagatttagg cttagaggat atggatggaa tagaggtaat aaaagctatc agacaatttt
     5581 caaatatacc aataattgta gtttcagcta gagagcaaga tagagacaag gtagaagtat
     5641 ttgatgcagg ggcagatgat tacttaacaa aaccattctc catagtagag ttattagcaa
     5701 gagtaagagt agcatttaga cattcacaag tagaagctca acaaaaagat gatgttaaat
     5761 ctacgtttga agtagataaa ttattgattg attttgataa aagaaaagtt attgttgatg
     5821 atgttgaagt ccatttgaca cctattgaat ataatattct ttcattactt gcaaagcatc
     5881 atggaaaagt gcttactcat aattttataa taaaagaaat atggggtagt gttataggga
     5941 atgagactaa gtctttaagg gtctttatgg caacgttaag acgaaaaata gaaaaacaac
     6001 ctgcaaaccc aagatatata attacagaag ttggtgtagg atacagatta aatgatgaat
     6061 agttgtaata tattaattat tagattatgt gcttattaat atatattaaa attatatagt
     6121 ttagttaaaa taagaatgaa gtgtatgtgc ttgttgtcag tttagcacat gcattttatt
     6181 ttttagcttt tttagtatca ataatttttt agtatcaata atatgaaata tgttttaaaa
     6241 taagtatata gtgtaatctg taataaaaac tttggttaaa taataaatgt atttaacact
     6301 taccttataa taaatttaat ataaatatta aataataatt attaaaatag tgtaaaataa
     6361 ttacagtaat gattataaaa aatttttaga atgtaaattg aaatttagga gtagatgtat
     6421 gtatatactg aatttaaata caagagagac tattgaagat tttagagata aattttatgt
     6481 agcagaaaat tcttatctta tattaagtgc tccaaaaaat ttaaaattgc ttaaggaaac
     6541 tttggatata gatgaaataa cttttaatga ttgtcttaaa tttgatgaaa taaccaaact
     6601 tgatttattt gataattacg attttttaag cctaaataca tttgaactaa gagatggaga
     6661 agctgttata gaagaggtaa atatgtattt atcagataat tttatattgg ttgtagtgaa
     6721 tgaagaacac tttttatttg aatttgtaaa aaatattatt ttgaagaact ctcaacttga
     6781 aaaaaatcca gttattaatc tatttaagat aaactacttg attcttagag aagttataaa
     6841 aaatggtttt gagtctttgg aaaaagttga agagttaatt cttcaaatag aagatgaaat
     6901 gatggacaac attaataaga atcatgtatc tagaataagt gatgtaagag gattaacaag
     6961 aattatagtt aagaatacta gacctctttt gtatattggg gatagaatag ttaaggaaaa
     7021 tataagatat ttaaaatatt caaatgtaaa aaaatataat ttagaaaatt ttcaaggcat
     7081 agattttgga attgacaaat tatacaattt cgcattgtca acacgagagt tagcagataa
     7141 attattagat atttattctt ccagagtagg tgagaaaaca aataacttaa ttacaaaatt
     7201 aaccttgtta acagccatat cagcaccact tacaattata actggtatat atggaatgaa
     7261 ctttagatat atgccagaat taaactggat ttatggat
//
LOCUS       DAEMHZ010000167         7288 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000167 DAEMHZ010000000
VERSION     DAEMHZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.168"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(192..1031)
                     /locus_tag="KPK72_003420"
     CDS             complement(192..1031)
                     /locus_tag="KPK72_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2490599.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1055..1813)
                     /locus_tag="KPK72_003421"
     CDS             complement(1055..1813)
                     /locus_tag="KPK72_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2490600.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1860..2333)
                     /locus_tag="KPK72_003422"
     CDS             complement(1860..2333)
                     /locus_tag="KPK72_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2490601.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHNVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDIKCFIQIVPDAKKIDVGTLL"
     gene            complement(2362..2778)
                     /locus_tag="KPK72_003423"
     CDS             complement(2362..2778)
                     /locus_tag="KPK72_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2490602.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2825..3877)
                     /locus_tag="KPK72_003424"
     CDS             complement(2825..3877)
                     /locus_tag="KPK72_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2490603.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4273..4953
                     /locus_tag="KPK72_003425"
     CDS             4273..4953
                     /locus_tag="KPK72_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2490604.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5086..5406)
                     /gene="sugE"
                     /locus_tag="KPK72_003426"
     CDS             complement(5086..5406)
                     /gene="sugE"
                     /locus_tag="KPK72_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG2490605.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5788..6615
                     /locus_tag="KPK72_003427"
     CDS             5788..6615
                     /locus_tag="KPK72_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2490606.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGSLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2474 a         1318 c          844 g         2652 t
ORIGIN      
        1 cctcattctg attaaaaatc tttctgagat actttttaaa atattaaata ctacatatag
       61 cttacataag tttaaaattt aatctattat aaaattaccc taattgccaa actttttaat
      121 ataaattttc caacatccta caaatataat caatagatat agaatcctaa cttacaccaa
      181 tatatattta tttaaaatag tcctaaaata cctgtaatag ctcccactac tatcagtata
      241 aacattattt ttattggaga aagacctttt tttaaagcaa agagtactaa taaagtaagt
      301 accaaaggaa ttaacccagg aaatatctta tcaagtaaat ccgtttgaag actaaattta
      361 aaatcaccac tagtataact tattggtgta gataaattaa taaacctacc tacaagtccc
      421 cccatgacta aggtacctag tatagaagcc ccttctataa ccttatttac tattccacct
      481 tgaagtatac tttcaacagc ttttttccca tacttatatc catttgtcca cattccaaaa
      541 gaaattgaag tcattattat atattgagct attacaaata atataggtcc tgcaattgat
      601 gctcctccct cagttaaacc aatacaaaca gccaacacta taggagttat aactccttga
      661 gttaaagtat caccaattcc cgctagtgga cccatcaagc ctgttttgat agcattcata
      721 ccatctccac ttatttcatc tgctccatta gctttttctt cttccatagc tattgttatt
      781 ccatgtataa cacatccaca tataggttct gtgttaaaaa atgctagatg agtctccact
      841 tcttgtttta actcttcctt atctggatat aattttttaa gtactggcaa cattgaatgt
      901 gaaaaagctg ttgattgcaa tctttcataa ttgtaattag attgtgcaaa gaaaaaccat
      961 cttaaccatg attttattac atctttcttg cttaacgttt tcttagatac ttcatttttt
     1021 atatctgaca taatattttc cccctcacat gtatttaaga tgtagcacta tcttttccgt
     1081 acttaattga agtaattgta agagcaagta ttactccaat aacagatatt actattatat
     1141 ctactttaaa ataaacagcc attagtattc ctaatatgaa aaagggcata gtaaacttgt
     1201 ttccaatcaa ttttaaattc atagcaatcc caagtgctgg caacatagcc ccaattacat
     1261 taaatacatg taacacatta tcattcatta cttccaacat accatctatc gcatttggac
     1321 caaaataaac aactaataat gctggtataa aactcattgc aaataacagt atctgtggtg
     1381 gtactatatt tataaatgct actctatcaa cattcccttt ttgaacctct ctatcagccc
     1441 aatgtgcaaa aaaagaattt actgtcatct ttccaatcca aagtacagtt gcaacagttc
     1501 ccaatggtac agctattgcc attgcagtac ttacatctag cttagatgct atagctaaag
     1561 cagtacctac atatcctgca aaagctgggt cccccatctg agctccccct gctgatataa
     1621 aacctagata tatcatattt atacctgctc ctataattgc tccttgaaca gggtctccta
     1681 atataattcc tgttacaaat ccagccaata gaggtctatt ccatgtaaaa aatccaacat
     1741 aagtcaacca cggactccaa gaaagataat aaaaaattgc tattaatact gcttgaatta
     1801 gtgaaatgtg catattcttc ctccctattt tcttttaaag atttacaaga tagtcttctt
     1861 tacaataatg ttcctacatc aattttcttt gcatctggta ctatttgtat aaagcattta
     1921 atatcttttg atattagctc tctaaatatt tctttttctt catctgacac agatatattt
     1981 ttataaaaac tttttctacc aagccctgct cccatacctc caacattcaa ttcctttaaa
     2041 tctaccccac tatttactaa acctaagaca gttttagggt atttgaccaa tactattaaa
     2101 ttttcatctc cattatgtga ttttaagact tcagcacctg attctacatt atgtacttca
     2161 accttaacag ttgatggagc tgccatttgt aaaacttgaa ctataaatgg gtcctttact
     2221 actaaatcat ccacgattac tatccttttt gccgatgtaa tcttacacca agcagtaata
     2281 acttgcccat gtattaatct atcatcaatt cttgttaatg ctatattact cataactatt
     2341 cctccagatt tttaatttat tttacaactt taaaacagtt ttaacatctt tgattcctga
     2401 tatcccaact tcctcacata aattcttcaa gtcttttaac ttcatagtat ctctgtttaa
     2461 aaataactct aaaagcatag gtaaatttac acctgtcaaa cattctaact cacatacctt
     2521 agcaaattta tttattataa tagcagatgt attaaatgga gttccgccat aaaaatcaag
     2581 caatactaaa accccttcat ccttatattt ctcaattcca tcttctaact ttctactaaa
     2641 ctcatctatg ctgtcaccat ggtttaatcc taaagtttct acattttcct gtttgcctac
     2701 tattaattca gcactttcta acattgcttt agccatattt ccatgtgtgg ctataattat
     2761 tcctgccata tgaatcatac ctttcttaac cttttttata tggattatta tttagtactc
     2821 taatctaatt atctatatta atcattatct taccaaagaa aatatccctt ttgtatactt
     2881 tttcaaaata agctggtact tcttctaaat ttattctatg tgttactaat ttttcaacat
     2941 tcatatcacc cttttgcata tgatacaatc cagcactcca ttcttttcct gggaatggaa
     3001 aggagtttcc aaaccaagtt ccctttacag ttaattcact tcttactatc ttttcaaatt
     3061 gctctctagt caaagctaca tccccatatg gtacacctgc atacaatact gtacctccct
     3121 ttttagctag caacagtact tgaccacatg tcagtggagt accagccgat tcaataacaa
     3181 tatctgcacc atctccatca gttaaccttt ttatttcttc tacaatattc ttttcttttg
     3241 catttataca aatatctgcc cctaactctt ttgccaagtc taacttctca tcaaatacat
     3301 ccacagctat tacttttgta gaaccaaata tttttgccca ttgtattgaa aataatccta
     3361 ttggacctgt accaagtact acaacagtat ctccaacttt agcttctgac ctaaaaagac
     3421 catgtcctgc tatacatact ggttctagtg cagcggcagt ctcataactt atctcatctg
     3481 gtatttttat taaatttctc tctttcacct ttgtatactc tgcaaaacaa cctccaagtt
     3541 ctttatttcc tattattgct acattattac atcttgaata caagcctttt ttacattcat
     3601 cacattcaaa acatggcata gctggacaaa cagcaactct atctcctatt ttaaaactcc
     3661 ttacttcttt accaacctgt gccacttctc cagaaaattc atgccctaaa atttctccaa
     3721 ccatatgtgg acctgtcttt gaatattttg atatatcaga accacatatt ccagctactt
     3781 ttactttaat tattacatca tctttttcta atatttttgg tacatccaca tcctccactc
     3841 ttgtgtctct tataccatag aatctaactg acttcactat taaccacccc tactttttat
     3901 ttgagaatat attcctattg tttatatgtt aaatatctaa agcaactttc atgccaatat
     3961 atgtaagcat tgtattcatg cattttacaa aattatcatt aaatgtgtgt caaagtgtgt
     4021 caaaagtttc ttatattaaa acttatgtgt gtcaatataa acgacactaa cattttatat
     4081 tagttctctt attataagtt ttataaaatg tacttttttt gttattttta taaaattgtt
     4141 cggaaatttc ccttctattt gtgatataat attaaatata aggtatcaat ataaattttc
     4201 atgcgaatat cagaactttt ttgacactaa tgccttattc aatataataa tttaactttt
     4261 tggaggttgt ttatgaaaaa agtgtatcaa ggaaagacta aagatgtttt tgaattagat
     4321 aatggaaatt acttacttaa atttaaagac gatgtaactg gtaaagatgg agtatttgac
     4381 ccaggcgaaa atagtgtagg tctaagtata gatggaattg gtagagctaa tttagagaca
     4441 tctgttaagt tctttgaaat tctaaacaat gctggaatta aaactcatta tgtaagtgct
     4501 aatttagaag aagcaactat ggaagttcta ccagcaaaag tttttggtaa tggattagaa
     4561 gttatttgtc gttatagagc tgtaggtagt ttcttacgtc gttatggttc atgtgttgaa
     4621 gaaggtgcta aactagattg ttacgtagaa gcaactttaa aagatgatga ccgttgtgac
     4681 cctcttataa catctgaagg tttagaagct ttaaacatta tgactcaagc tcaatttgat
     4741 tcaatgaaaa gcatgactca aaaaatttct aacatagttc gtgatactat agctgaaaaa
     4801 ggtcttgagc tttatgatat taaatttgaa tttggattct acaatgatga agtaatctta
     4861 atagatgaaa tagcatctgg aaatatgcgt gtttataaag atggtgtaat agttgaccct
     4921 atggatttaa ctgctctttt attagataaa tagtatatac atataattta caaatataat
     4981 aaacttataa ataagataag ctatgaagta aagacttttt aaatgttgat tatttttaaa
     5041 cattttctcg cctttaattt catagcttat ttaaattaat atactctaat tagtagataa
     5101 tagctttaac cctacaattc ccaccacaat aaaacctata catgcaattc ttgatatact
     5161 aataggctca tttaaaatag ccatgcctac aatagctgta ccgacggtac caattccagt
     5221 ccatattgca tatgaagttc caagtggaag atgttttagt gctaatgaca aaaaataaaa
     5281 acttacaatc attccaacaa cagtaagtat acttggagtc aatttagaaa atccatgtga
     5341 attttttagt tgaaaagccc aaaacacttc aaacaaacct gcaacaaaca aaaataacca
     5401 tttcataaat tcatccctct tttcaaatat gtctcttatt tttccaataa aataacctga
     5461 gaaatattat aaaatatctc ccaggttttt gtccttccgt gttcacaaca aaaactatgt
     5521 tgtgggtttt atcttggacc agaccaatca ttaataattg cggaacccta taaaactcta
     5581 ttaattattc tattttatct ataataatac tatcaacata tataaaaaaa gtcaatatat
     5641 gggttccatc tataataaaa ataactttta tatacctttt aatattctta ttcacttctc
     5701 tccataccca tttgggtcaa tctaagaata tctaattttt atttaatatg ttataataac
     5761 ataaaaatat tgaaaggagt aatttctatg aatttctata aaattgcagt atgtcagatg
     5821 attactacag aaaataaaat tgaaaatatc aatcatgctg tagatatggt aacagaagct
     5881 gccatcaatg gagctaaaat tgtagttttg ccagaaatgt ttaactgccc atatgaaaat
     5941 aaatattttc ctaaatttgc tgaagaatat ccaggtgaaa caacaacgat attaagtaaa
     6001 ttagctgaga aacatggaat ttatctagtt tctggtagta ttcctgagtt agaagatgga
     6061 aaaatctata atacttgcta tgtatttgat aaaaatggat ctctaattgg aaaacatcgt
     6121 aaaatgcact tatttgacat tgaagttaca ggaaaagtta gttttaaaga atcagatact
     6181 cttacagctg gaaatgatgt cactgtaata gataccgaat atggaaaagt tggtatagct
     6241 atttgctatg atattcgttt ccctgagtta tcacgtttaa tggctcttaa aggtgctgaa
     6301 atcgttatct tacctgctgc atttaatatg actactggtc ccgcccattg ggagttatct
     6361 ataagaatgc gtgctttaga taatcagata ttctatgttg gtgctgcacc tgctagaaat
     6421 atgaatgctt cttatatagc ttttggaaat tcaagaattt cagacccttg gggaagaata
     6481 attgctcaag ctgatgaaaa agaatgtatt atttatgctg acattgatag agatttaatt
     6541 cctgacataa gacaacagtt accacttctt aagcacagaa gaactgattt atatgaactt
     6601 aatactttga aataaaacag aacataaccc cataaatcat aaattaagtg ttaaaaaaat
     6661 ttatggggtt ctcattaatt tgatttaaaa ttaatttcat aaaaataatg ctaatattaa
     6721 tttaattaaa taatactaat aattactaat gattatgcta attatcagca tttactaatt
     6781 taatcactat tagttatttc ttttattaaa tatctttact tttttactta ttatgtcttc
     6841 tagctttaca tttggattat ggtatttctt tctattttta gtcacatctt tctattgaat
     6901 tacttgctta aaacaccaat gcacacatgc cagataatgt tcacaattat gtagaaatct
     6961 tgctctacct tcttcaatgt tgatattatc tctcctagtt gatattatct ttcctaaaat
     7021 ttcttttaat taaaaaacaa cattagttga tatataaagt atattttaag caactgaaaa
     7081 aaacaataat aattgatata tcaactaatt aatgtagatt aattcatttt tataatttat
     7141 atacttttta attaatctac cttatttaat taaaatcctt cctttctatt tatccatttt
     7201 caacttaaat tataagattt catcagctat atttttgcat accaataagt attaaatatc
     7261 aaatatatat tctattaaaa tttctaat
//
LOCUS       DAEMHZ010000168         7164 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000168 DAEMHZ010000000
VERSION     DAEMHZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.169"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..82
                     /locus_tag="KPK72_003428"
     tRNA            6..82
                     /locus_tag="KPK72_003428"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:His,seq:gtg)
     gene            91..166
                     /locus_tag="KPK72_003429"
     tRNA            91..166
                     /locus_tag="KPK72_003429"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Lys,seq:ttt)
     gene            174..247
                     /locus_tag="KPK72_003430"
     tRNA            174..247
                     /locus_tag="KPK72_003430"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Cys,seq:gca)
     gene            255..331
                     /locus_tag="KPK72_003431"
     tRNA            255..331
                     /locus_tag="KPK72_003431"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Arg,seq:acg)
     gene            344..419
                     /locus_tag="KPK72_003432"
     tRNA            344..419
                     /locus_tag="KPK72_003432"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Val,seq:tac)
     gene            495..908
                     /locus_tag="KPK72_003433"
     CDS             495..908
                     /locus_tag="KPK72_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490607.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1217..4648
                     /locus_tag="KPK72_003434"
     CDS             1217..4648
                     /locus_tag="KPK72_003434"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG2490608.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4890..6818
                     /locus_tag="KPK72_003435"
     CDS             4890..6818
                     /locus_tag="KPK72_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2490609.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         2978 a          786 c         1346 g         2054 t
ORIGIN      
        1 ttatggtggg tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt
       61 tcgactctca ttatccaccc caacttatat ggtctattag ctcagtcggt agagcacctg
      121 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggcggcgaca
      181 tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc
      241 gcctccaata aaatgcgctc atagctcaac tggatagagt gtctgactac gaatcagaag
      301 gttaggggtt cgagtccctt tgggcgcacc atatgaatag cgagggatta tagctcagct
      361 gggagagcac ctgccttaca agcagggggt cacaggttcg agccctgtta atcccaccag
      421 tgcaaacctc tagtaaaact actagaggtt ttatttttga ttgaaaaagt ttttatattt
      481 aggaggtaaa aaatttgtat atagtaatag gaaatgaaat agttgatagt gaagaactta
      541 aaataattat agataaaaat agtgaattta aagttgaaaa agatttaagt aaaagcacaa
      601 aaagagaaga tgtaatcgca tatcagttaa gtatagattt aaattaccta gattctctta
      661 taaatgagca atgtaattta gccagtctat ctgatgaaga gaaatttgat gaatatatga
      721 ctttgtctga tgaacttgca ctggatttag aagagttaat gccaaaatat acaataataa
      781 atgctagagc atataaatta gacgaggtag atggaattgt aaaaataata ctagctgtag
      841 cgtatgcaga tttagggcac ttaaaacttt ctgatgtagt aaagaggcta tctagacaag
      901 tagattagga tatgaatatg aactatatta aaaaaatgta taaaatctta cgtaaaaatg
      961 aaaataatta tgtaaacata gaaaaaaaca gtataaaatc aagtcaattg tagaaatttt
     1021 cacataaatt catctgaaat tctataaaaa atgccttaat tttaatgagg taaatcgagt
     1081 ttacacagtc taaaaattac ttatatttat aaatttatct tattaattaa ggaactttct
     1141 taaacatctt caaaaataaa gtattttagt ctataatctt taatattaca caaacaataa
     1201 aggagaagaa tagagtatgc ttaaaaagtt taataaaata ctagtggcta atagaggcga
     1261 aatagctata agaatattta gagcctgttc agaattgggg ataaaaagtg ttggtatata
     1321 cagtaaggaa gataagtatg gattgtttag aacaaaagcg gatgaatcat atttgatagg
     1381 agaaggtaaa gggccaatag atgcatattt agatatggat ggaataatag acctagcaaa
     1441 gagaaaaaaa gttgatgcta tacatccagg atatggtttt ctagcggaga atgcagaatt
     1501 tgctcgtaaa tgtgaagaga atggaattac ttttatagga ccatcatcta aggtaatgaa
     1561 tatgatgggg gataagatta actctaaaaa aatcgcaaaa gaagttaatg ttcaaactat
     1621 accaggagta gaaaaagcaa taagaagtac tgaagaagca aaggaagttg caaacaaaat
     1681 aggatatcct gttatgataa aagcatctaa tggcggtggc ggtagaggta tgagaattgt
     1741 acatcgcgaa gaagacttag agcttgaata tgaaactgct tgtagtgaat caagaaaagc
     1801 ttttggagaa gatattatat ttatagaaaa atatatagct gatccaaaac atatagaagt
     1861 tcaaatatta ggtgataatt atggaaatat agttcactta tatgaaagag attgttcagt
     1921 tcaaagaaga catcaaaaaa taatagaata tgcaccagct ttttcgttag atgataaagt
     1981 aagaaaagag atatgtgaag atgcagttaa gttatcaaaa catgtgggtt attctaatgc
     2041 gggaacatta gaatttttag tagatgcaaa tggtgggcat tattttatag agatgaacac
     2101 aagagtacag gttgaacata ctgtaacaga gatggttaca ggaatagata tagttcaaag
     2161 tcaaatacta atagcccaag gttacagctt agatagtgaa gaaattaata taaaatcaca
     2221 agatgatgtg gaaataagag gttactctat ccaatgtaga ataactacag aagaccctaa
     2281 gaataaattc atgcctgata caggtaaaat tcaagtttat agaactggtt caggatttgg
     2341 tataagacta gatggaggaa atggatttac aggggcaaac ataagtcctc attatgatag
     2401 tttattggtt aagacaatat cttgggatag aacattccaa ggagcaataa ataaaactat
     2461 aagatctata aaagaactta gagttagagg tgttaaaact aatgtaggat tcctagttaa
     2521 tgttttaaat aatcctatat tctcaaatgg taaatgtagt actaagttta tagatgaaaa
     2581 tccagattta tttgaaataa cagaaagtaa agacagagga acaaaattac ttcaatttat
     2641 aggagatgta attgtaaatg ataatgcttg taaagaaaaa ccattatttg atgcattaca
     2701 tgacccaaga atggataagg atggttcaaa atcagaagga agtaaaatac ttttcgataa
     2761 gttaggtaaa tctgcatata tagaaaagat aaaaaatgat aagaaattac tattaacaga
     2821 tacaacaatg agagatgcac atcaatcatt attagcaaca agaataagaa catatgactt
     2881 attaaaagct gctaagccaa cagaaaaata tcaaaaggat ttattctcac ttgaaatgtg
     2941 gggaggagct acttatgatg ttgcatatag atttttaaaa gaatctcctt ggcgtagatt
     3001 acaaaagcta agagaagaaa taccaagcat aatgttccaa atgttgctta gagcatcaaa
     3061 tggagttgga tataaaaact atccagataa tgtaatagaa gaatttacaa aagaatctgc
     3121 tagacaaggt atagatgtat ttagaatatt tgattcatta aactgggttg aaaatatgaa
     3181 accatctatt aatacagctt tagaaactgg aaaaattgtt gaagcaacta tgtgttatac
     3241 aggtgatata ctagataaga ctaaaactaa atacaatcta gaatattata taaaaatggc
     3301 acaagaatta gaaagcttgg gagcagacat aatagcaata aaagatatgt caggtcttct
     3361 taaaccatat tcagcttaca cattagttaa agagttaaag aaaaatgtaa aagcaccaat
     3421 acatttacac actcatgata caagtggaaa tggtgtagca acttgtttaa tggcgtcaga
     3481 agctggagta gacataattg atgctgcatt agaatctatg gcaggactta caagtcagcc
     3541 atcacttaat gctatagtag aggcacttaa gaatacagag agagatacag gaatagattt
     3601 atttggatat gatgagttag gaaaatatta taaggattta agaaaagttt ataataaatt
     3661 tgaaagtgat ttaactaatt cttgtgcaga aatatataat tttgaaatac caggaggcca
     3721 atatacaaac ttaaaacctc aagctgatag tttaggatta gttaatagat ttgatgaagt
     3781 taaagaaaaa tataaagaag ctaatgaagt tgtaggagat ataataaaag taactccatc
     3841 ttcaaaagtt gtaggagact tagctatctt tatgactaaa aataaattag ataaagacaa
     3901 tataatagaa gaaggtaaaa atttatcatt cccggattct gttgtggatt actgtaaagg
     3961 tatgattggt caaccagaag gtggaatacc aaaagattta caagaggtag ttctaaaagg
     4021 tgaagaagct ataacagtaa gaccaggttc attattacca gcagaagatt ttgatgaaat
     4081 agctaaatat ctaaatgaaa agtatgatat aaatgctaat ataagaaatg ttataagtta
     4141 tgctttatat ccaaaagtat atgaagatta tatcaaacat ctacaacatt ataatgatat
     4201 atctaaactt gaaagtgatg tattcttcta tggattaaat aaaaatgaag aatgtgaagt
     4261 agaaatagaa gaaggtaagg tattaacaat aagattagtt gaaattggtg aagttaagga
     4321 aaatggattt agaactatag gatttgagtt aaatggaatg gttagagaag tagaaataaa
     4381 agataagaat ttctctggaa aaattaataa tgttgaaaaa gctgatatga atgaccctct
     4441 tcaaattgga gcaagcatac caggaaaagt tataaaaata atggttaaag aagaagatga
     4501 agtaaaagca aatcaaccat taatcgttat agaggctatg aagatggaaa ctataatagt
     4561 tgctaagact gatggagtaa ttaagtcaat caaagtaaaa gaagatgata tggttgaaga
     4621 taaacaatta ttaatgataa tgaagtaatt taattaaaat aaattaaaaa gcactctgaa
     4681 atgtttaata aaaaatgttt tagagtgttt ttatttattt taattaaata tttgttaaaa
     4741 aaaagaggac aattacctcc agatgtagaa ataaagctag taaggaaaat gttatctaaa
     4801 aattctaaat atagtaaata ttttccataa tatttcttag gaaataacag caaagataaa
     4861 atacataaat ttaaaaggtg ggtgcttata tgaaggttta tgatagtaaa atgttgagaa
     4921 atgtagcggt attaggacat agcggatgtg gaaaaacaaa tctaatcgaa actatagctt
     4981 acacagcaaa cactaataag atacctaaat taacagacaa ggtaaatatg acttatagca
     5041 tgggattaat acctatagag tataatgatt ataagtttaa cttattggat accccaggat
     5101 attttgactt tagcggagac gttgtgtcat ctcttagagc aagtgatgca gcaataatag
     5161 taatagatgc aacagcgcct attcaagttg gtacagagaa atcattagaa ctaactgaga
     5221 gtattcctaa gataatgttt ataaataaaa tagataatga aaaggcaaga tacaaagatg
     5281 caatagcaat gttaagagaa aaatacaata ataagatagt tcctatgata agtcctatat
     5341 acaaagataa aaactttgta aagttacata atgtttttga aaacatagat gatttagaag
     5401 gtgaatttaa ggaacaagct atgagtgtta aagaagcatt aatggaactt atagcagaaa
     5461 ctgatgacca aatattagat aaatatttta atggagaaga actaactaca gaagagatac
     5521 aaaaaggtat tattataggt atacaaagag gtgatattat acctgtaata tgtggttcaa
     5581 ctataaataa tataggcaca aaggaaatat tagatacaat atcaagttat ttagaaccta
     5641 tctttacaga ggaaagcaaa ccatttaggg gattagtatt taaaactatg gttgacccat
     5701 ttgttggaaa gatgtcatat ataaaaatta ctgagggtgt actttctaag gataaagatg
     5761 tttttaatat aaataaaaat gtaaaagaaa aaatagctaa tatatataca ttgagaaata
     5821 gtgaattagt agaaatagaa aaagctaaag ctggtgatat agtggttata actaaagtta
     5881 attctctaaa aacaggagac actatatctg cagataaaga tgcagaagct ttagaaaaga
     5941 tagattttcc taaaccacaa atatattatg cagtaactcc taagaataaa ggtgatgaag
     6001 aaaaagtagc atctgtatta aataaactag tagaagagga ccctacatta cattggtata
     6061 gaaatacaga gacaaaacaa gcacttttag gaggtcaagg agaacttcat ataaaaacaa
     6121 ttaaaaataa aatgaaagat aagtttggtg ttgatgttga attgaatgac cttaaagttc
     6181 cttatagaga aactataaaa ggaacagctg atgtacaagg aaaacataaa aagcaatcag
     6241 gtggtcatgg tcaatatgga gacgtaaaaa taagatttga aagatgtgaa tctgattttg
     6301 aatttacaga agaaatattt ggtggttctg taccaaaaca atatatacca gcagttgaaa
     6361 aaggtttaaa agactctatg caaaaaggta tattagcagg atatcctgtt acaaacataa
     6421 aagctacgct ttatgatggt tcataccatg atgtagactc ttctgaaatg gcctttaaga
     6481 tggcagcaag tgcagccttt aaaaaaggta tggaagaagc acacccaata ctactagaac
     6541 ctataatgaa acttaagata actgttccag aagaatatat gggagatgtc atgggtgata
     6601 taaataagag aagaggaaaa atatttggaa tggaaccaga tgacaaaggg aaacaaataa
     6661 tatttgcaga agctccacaa gcagagacat ttaaatatgc aatagattta agagcaatga
     6721 cacaaggtag aggatacttt gaaatggaat tagagagata tggagaggtt ccatcacagt
     6781 ttgcagaaaa aataattggg ttagctactg caaagtaatg aacaaaataa atagaaaaat
     6841 aattaatata tattattaaa aatattttta gggttttaac taggtatagg tttatgttag
     6901 gtaaacttat acctagtttt tatacatttt tgatgaataa aaaaatatat aatactttct
     6961 taaatatttt aataaaaaga aagtattata tattttttta ttattatttt tacaaatgtt
     7021 tagtaataaa gatgagaagg tatatttaaa atataaagta tttaaagttc tgaaatctaa
     7081 tcatgaagca aaaaattagc atttggttat caataaatat aataaataaa aactcataat
     7141 taatgaaaga ataaattcat atct
//
LOCUS       DAEMHZ010000169         6895 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000169 DAEMHZ010000000
VERSION     DAEMHZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.170"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..2359
                     /locus_tag="KPK72_003436"
     CDS             2..2359
                     /locus_tag="KPK72_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG2490610.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKIGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2445..2762)
                     /locus_tag="KPK72_003437"
     CDS             complement(2445..2762)
                     /locus_tag="KPK72_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG2490611.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(3348..4619)
                     /locus_tag="KPK72_003438"
     CDS             complement(3348..4619)
                     /locus_tag="KPK72_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2490612.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   5266..6282
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5266..5294
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         2602 a          777 c          981 g         2535 t
ORIGIN      
        1 tatggataag gttaatttag aaaggtatag ttgtgatata aatgttggtt taaataaaaa
       61 tcaacttcaa agtagaatag atgataattt aattaatgca tttgataatg gaaaaaccaa
      121 aacatataaa caaattttta aagacaatat atttacactt tttaatctca taaatattgt
      181 tttagtctgt ttattagttt cagttggttc ttttaaaaat accttattta tatttattgc
      241 tgtaattaat actattatag gagtaataca agagataaga gctaaaagat tattggacaa
      301 tctatctgtt atagtttcaa ataaggtatc tgtcataaga gaaaaagaga aaaaagagat
      361 tgatgtacaa gaattagtat tagatgatat tatgatttta aaaataggta atcaaatttg
      421 tgcagattca aaagttttaa atggaaaatt agaggtaaat gagtctttgg ttacaggtga
      481 atctgatatt attataaaaa ataaaggcga ttttttatat tctggaagtt ttgtagtttc
      541 tggagaagct tttgtaagag ttgaaaatat aggaaaagat aactatgcaa ataagatagc
      601 taatgatgca aagatttcta aaaaacataa ttctcaactt agaaattcat taaatgcaat
      661 attaaaaatt gtaggtataa taataattcc tctaggaatt atattgtttg caaaacaaca
      721 ttttggaaat ggagattcta tagctacatc tgtagtaggt acagtggcag ctgtaaatgg
      781 tatgatacca gagggattaa cattacttac gagcattgct ctagctgtag gaactataaa
      841 acttgctagt cataaaactt tagtgcaaga actgtactgt gtagagactc ttgctagagt
      901 tgatacacta tgtcttgata agacaggtac aattacagaa ggtaaaatgc aagttgatga
      961 tatagtaatg ttggaagaag tggatattaa tgaaataatg gggaatatat gtaattctct
     1021 caaagatgat aatgcaacac taaatgcaat aagagataag tataatgttt tagatacgta
     1081 taaagctaag aatttaatac cattttcttc agaaagaaaa tacagtgggg ttacctttga
     1141 aaataaaggt acatatttat taggtgcatt tgaatttata tttaaggaaa aaaataaaaa
     1201 acttaggata gaagttgaaa gatattctaa aaaaggaaac agagtaatag ttttagctca
     1261 tagtgattct tatatggaag ataataatat tcctgaagat ataaagccac tagcttttat
     1321 attgatattg gataaaatta gagatgaagc taaggaaact ttagaatttt tttacaatga
     1381 aggtgtagat ataaaaatta tatctggtga caatcctata acagtaagtg aggtagctag
     1441 aaaagcaggt cttaaaacag ctagtaattt tattgatgca acaacactta aaaatgatag
     1501 tgatatatac aaagcagttg ataaatacag tgtttttgga agggtaagcc cacaacagaa
     1561 aaaacaaatg attttagcat taaaaaaaca aaatcataca gtagctatga ctggagatgg
     1621 tgtaaatgat gtgctagcac ttaaagaagc ggattgtagc attgccatgg catctggaag
     1681 tgatgttact aagaatgtgt ctaatttagt cttattagat tcaaactttg catcaatgcc
     1741 aaaggttgtt atggagggaa gaaaagtaat taataacatt caaagagcat cttctttgtt
     1801 tttagtaaag acaatttttt cattcttctt gtcgttatta acattattct tcttcagtag
     1861 acagtatcca tttgttccta tacaattatc actcattggg gctgtaacag tgggtatacc
     1921 atcattcttc ttagctcttg aagcaaacaa aagtagaatt tcaggaaatt ttttactcaa
     1981 tatattaaaa aatgctttac caggggcact atgtgtgaca gtaaatgtaa ttattgttta
     2041 caatatagtt gaatatttag gttatagttc aagtgtattt tccacaatgt gtgctatatt
     2101 aactggtgta tgtggtcttt taatattaag acaacaatgt ttgccattta ataaagaaag
     2161 attattttta atagtaagta tgactattgc atttgttatt gggattctat ttttaggtgg
     2221 attattctca tttgtagcat tgagtagaga attaattctc ataactatgg cattagcaat
     2281 attaatgcca attatgataa aagttatgtt atttgtctta gatgagatat tagagacaat
     2341 tgttccagat ttaaattaat tttaaaatga tataaataag agcagtttta gttgaactgc
     2401 tctttttaga tgtgtttaat tttatgtaag aatatgtatt tttattaatc agctatatat
     2461 gagcaacagt agtcaacaac ttcattaacc tttaagtcaa aacttgaatc actaggaaca
     2521 ttaaattttt gcccttcttt ataagttaca aactcattgc ttccaggcag ttttacatcc
     2581 atacttccag caagcatctc cattatttct tcttctctag tagaaaaagt gtattctcct
     2641 ggcaacatta atcctaaagt ttttctttct ccattaggca gtattattac tctgctactt
     2701 accttaccat caaaataaac gtttgccttc tttactgcag taacattctt aaattcactc
     2761 attagataaa acctccaaaa ttatttgtac atatatatta tataaaaata atgtaaaaaa
     2821 tgctatatat ttacaaaaaa aatttagtat ttattaaaaa aaattaataa gaataaaaaa
     2881 gagatgaaat tgcttaaatt gataaaaaat ataaaataaa ttgcaaagta agcaagaaaa
     2941 gaataagttg aagagttata tattataaat attaaaaatt ttagtattaa taaaagagat
     3001 attacattaa tatgaaaata taggagatta gaagtgtttg ataattacag tacatgttag
     3061 atatatcatt gtaaattaac atagctttga atataataat gttcaaaagg caatattttt
     3121 gaggtctgaa tccaataata atatagtctt atgattgtag atatcaaaaa atataagtta
     3181 ctaagaatac gttttgtaga tttatatatg attaataact aaagctataa ataaacaaaa
     3241 taaaaagagt atgtttcaac ctatagcttt tagatttaaa atatactctt ttattaatta
     3301 tagttgtata aattaaccat aatatataat taaaattttt attataatta tatattaggt
     3361 tcttcaatta gttttccaca tacatatcta tgaatgatat ttctatcatc accaacatag
     3421 ataaatcttt caagtctttc aagtatagaa taattgtcat gatttaaatt tgaatcatca
     3481 ataatcaatg catcaaaatc ataaccttct tcaaagctac caacttttcc aaagaaactt
     3541 ccaccacttt tggttgcaat ataaaaagcc tcagataaag ataagaaatt atacttctta
     3601 ccagaattaa cccaatataa tttagataat tgtattgcag ataccattgc tttaaatatt
     3661 gacaatgtat gtccaccgct tatatctgat ccaagtgcta atctaatatt attatcgatt
     3721 aattttctga taggcatagc accactacca acattaaagt tagaagctgg acagtgaact
     3781 gccataacat tattttctct cattaaatct atttcatcct gagaacaatg aacacaatga
     3841 gccattaaag taggagtttg accaaataaa ttatatctat tatatgcatc tccatagaat
     3901 ttagattcag gttctagact tctaacccat tcaatctcat cataattttc agataagtgt
     3961 gattgaatag gaatattata ttttaaacat aaatctccta aaccttttaa aagttcactt
     4021 gtacagcttg gtataaatct aggtgttatt attggtttta caatagaatc tgtatcacta
     4081 tacttattta taatttcttc tgtatcagta atagaatcct gagtgttttc taataaagta
     4141 tcagggcaat tcatattcat atttacttta ccaacgtatg ctccaagacc aacttctttg
     4201 aatatatcca taagtttcat agaggcttct ttatgtactg ttgcaaacac tgctattctt
     4261 gtagttccag atttccaaag ttcttttaca aattttttat acatttttgt tgcatattct
     4321 atatctttat aatttgattc ttctggaaat gtataagttt caagccatgg caaaagctct
     4381 ttatccatag ccattccaag atttttaaat tgagaggcat gggcatggag gtcattcata
     4441 ccaggtatta taagcttatc agtgtaatct actactttta ggtttttgta ttccttaggt
     4501 atctctttaa atattccctt tactttacct ttttccagca taatataact attttcaaat
     4561 actgtgaaag tttctgaagt ttttgtaaaa attatatttc cttttagtac ttttaacata
     4621 taggtctcct tagttttaaa tttgttttaa tatatgtagg taaaattttg ttttacaaat
     4681 caaaatttta ccacatgtat tatatcaaat ctaaactata aatactatta gataatagca
     4741 aatattagtg aaatacagtt gtttactatt attatatata actgacattt aagtgacatt
     4801 taagaaaaat ataatgccta cttacataaa atggaatgtt atttaaagag aactttgatt
     4861 atattttcag aaactttttt atccatatcg tttaaaagat gagaatatct atttatagtt
     4921 atttttatat tagtatgtcc taatctttca gatatgattt ttatattagt tccagctaga
     4981 agaagaatta ttagaataga taatatagta agtatttaca aatatgtagg tgttcttaaa
     5041 ttgataaatt attccatttt aattttatag tttgaatttt atgatataat aaaaatatat
     5101 aaattttgca gtgagcgata tttttgataa agtagggttt aacagttgca atgtaaggga
     5161 ttgagggtgt gtgataaatg ttatcaattg cactactcat ggttcactgc aaatttgaga
     5221 gagttgtatg tgtgtaagta ctgaaaatac ttagtttatt ttggggtttt atattaacta
     5281 agtggtatgt aaattcaact tctgtttgtg tctttgaatt aattgttcca ggttttatat
     5341 taactaagtg gtatgtaaat agagtactac tctgtaatat atactattga tatacacagt
     5401 tttatattaa ctaagtggta tgtaaattaa atgaaaacta ttaatgtaga aaaaaagatt
     5461 gatagtttta tattaactaa gtggtatgta aattttaaat ggtgtgaatg gtgaaaggca
     5521 cacaacacgt tttatattaa ctaagtggta tgtaaattta tcgtgcatag ttaatattat
     5581 agtataagtg taagttttat attaactaag tggtatgtaa atatgtaaag tttagcaaat
     5641 tcaatagtat ccatattgtg ttttatatta actaagtggt atgtaaattg gacacaacaa
     5701 aaatttttca aaccttttaa aagagtttta tattaactaa gtggtatgta aataatttca
     5761 gtgctaaaaa tttagttaca gaatataaag ttttatatta actaagtggt atgtaaatta
     5821 taaaactacc ttcctatagc tcctaggaaa ataacgtttt atattaacta agtggtatgt
     5881 aaatcctgta gcacctttta ttatattttg aacgctacca cgttttatat taactaagtg
     5941 gtatgtaaat tgttgaaaat aaagacttta cagtagttac aacactagtt ttatattaac
     6001 taagtggtat gtaaatgccc ctaaccatgc aacacggcga caaactattt caagttttat
     6061 attaactaag tggtatgtaa attagaaaat aacgtcagta gtattagcag gtaaatctaa
     6121 agttttatat taactatgag gtatgtaaat cttatactta gttagaacta tatatcgaca
     6181 caaatatgct ttatattaac tatatggtat ataaattgca atttttattc gttgtccaat
     6241 ctctttgaaa tttgttttat attaactaag tggtatgtaa atttgcaaca agtataggta
     6301 aaatacccca ataatttata cagcatttta tcctttaaaa tataattatt ttttatcatt
     6361 tgtagtaaat aattaccaga taacattgac tttagtttta atgattaaaa tataaaagta
     6421 gaataattat aaaaagtatt gaaaaattta taaatatata taataaaacc taatgacaag
     6481 atattagata taaaaaataa ttaccttata aatagattga aatttatgaa tattcatact
     6541 ataatttaaa tataaggaga tgccctttga aaataaaaat taaaaaatat ttaatgctat
     6601 tacaatagga actagaacta cacttaataa atatacagaa attagatttg gctcaatata
     6661 aaatacaaat atagaattta ggtgtttttt atgaaaaaat ttttatatgc tttatatagt
     6721 ttcattgtta ttatagctaa ttttagatta aaagaaaaaa attataactt tattttatta
     6781 gcaatctttt ttatattgct gttaattcaa aatggagttt taaaaacagg ttatcaaaat
     6841 agagctacat tttatcaaga taattatttt aatagagagg ataaaaatga taaga
//
LOCUS       DAEMHZ010000170         6844 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000170 DAEMHZ010000000
VERSION     DAEMHZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.171"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            354..1628
                     /locus_tag="KPK72_003439"
     CDS             354..1628
                     /locus_tag="KPK72_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2490613.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGYKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1835..2152)
                     /gene="trxA"
                     /locus_tag="KPK72_003440"
     CDS             complement(1835..2152)
                     /gene="trxA"
                     /locus_tag="KPK72_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2490614.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2419..3171
                     /locus_tag="KPK72_003441"
     CDS             2419..3171
                     /locus_tag="KPK72_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG2490615.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLRILNKN
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3158..4039
                     /locus_tag="KPK72_003442"
     CDS             3158..4039
                     /locus_tag="KPK72_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2490616.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4265..5620)
                     /locus_tag="KPK72_003443"
     CDS             complement(4265..5620)
                     /locus_tag="KPK72_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2490617.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2627 a          965 c          856 g         2396 t
ORIGIN      
        1 aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta tttctaatta
       61 gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa taagtgacta
      121 tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa aatcaagtga
      181 gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat ctcaacaaga
      241 attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc ttgattaaaa
      301 cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt tttatgcaat
      361 tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact aaaaatggtg
      421 aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta aaaaacgtag
      481 tggataactt aaactgtgag attccttggc ttacttatat taatgctcca ggaataatct
      541 cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa cttattgatg
      601 gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga ataaacttct
      661 tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca gcaatggagc
      721 atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt ggaattacag
      781 aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat gctggtaatg
      841 tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact cctatatatg
      901 aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt gtgcaagtaa
      961 ttcagcataa gcctttttca atgatgccac atgaatgtga tgagcatata gattttgttg
     1021 tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta ggacctaaaa
     1081 aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa tttgtaaatg
     1141 ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac ccagagtatt
     1201 ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt ctaaacagaa
     1261 tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt aaaataccta
     1321 atataataat atatggatat aaatcaaata atgtaattat cccaaatata tcttttaata
     1381 taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt ggaatagaaa
     1441 ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt ataagtccaa
     1501 aagaggcata cgtcagattt atgagggaaa atcctgtagg tcttgttagg ataagcttag
     1561 gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa actttggctt
     1621 ttaaataaga tttttctatt cttttttatc aataaataga gagtagtttt gttttcacaa
     1681 aaaagcaaat tatatcaaca gtcaatttca ttatcaatta gacactcatt tgtaactata
     1741 caaactgttt tacactatac aaacaaaaaa ctcattacaa tgagtttttt tgtttatata
     1801 tgagatttat tttaattact tcaatttact ttaattacat atatgattca attttgtttt
     1861 ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca tccttaaata
     1921 ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata ctttggtcta
     1981 tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct tcatatattg
     2041 gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact actaaaccat
     2101 ctttattttc tacttcattt ataaattcat tattatttat tatttttacc ataatataca
     2161 tctcctttat tatcttcttg atattaatat actacgccct atataacagt tcaattaggc
     2221 acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag atttataaga
     2281 ttaaatattt atgagactaa atatttctaa tatattttat ttttttctta atatagttga
     2341 cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt actaatcttt
     2401 tatcaaagga gtttctatat ggataatata attaatgaat tacaaaatat aggatttact
     2461 aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac aggatatgaa
     2521 ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac aaagctatta
     2581 aacaataata taattatcaa tgttggaact gaccctatta agtacatacc aataaaccct
     2641 aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa tctaaagaat
     2701 agcttacgga tattaaataa aaataaaagt gtagaatacg tattaaacat aaaaggagat
     2761 aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg caaaacaaga aattataatt
     2821 tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc ttacaaaaaa
     2881 aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct tgataatatt
     2941 catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa aggaattatt
     3001 ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag tgaggccatt
     3061 tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat aaaacatgaa
     3121 atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta agattaaaca
     3181 agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg ttactgatgc
     3241 ctctaatatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt tatcagaaac
     3301 tgcatttgtt tcagagtcaa acgttgcaga ttttaaggta cagttcttca caccaggttt
     3361 agaagttgat ttatgcggtc atgcaacaat aggtactttc catgcacttt tggaagaagg
     3421 gaagcttaat actaataaga gtatatttta tcaagaaaca aaagctggtg tattatctgt
     3481 agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata cacctgaatt
     3541 tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc atgaagatag
     3601 acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg tatttggtct
     3661 aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca aaaaatttag
     3721 tggtgataat aatataattg gtataactcc attttgtata gaaactatag ataaaaagtg
     3781 tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc ctgtatgtgg
     3841 aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag ataaaactag
     3901 ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg taaatataac
     3961 tagagaaaac aatgacataa cttctgtaaa aattggagga agtgcttata ctgttttaag
     4021 tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac tagctaacta
     4081 aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa atattataat
     4141 tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt aattagttat
     4201 aaatcaatct aatacaacat aattacagta gattaaccta taaaacataa taattgctta
     4261 ctgattattt atcgtcctca acaagtctac ttaatgtctt atcagataag aataataata
     4321 tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata tcaaatttaa
     4381 gagtaaatgc atatatatat ccatatcctt taccagctat aaaagtagca aatgtccata
     4441 ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa gagttaccaa
     4501 gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt atccatagta
     4561 tactagcttt agcagtttca ggagctccaa ctcctcttga aaattctgca ccaacaagca
     4621 ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat aaactcatat
     4681 ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt cctaatacta
     4741 tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat cctccaacat
     4801 tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga taccagaata
     4861 cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc ttcttatcca
     4921 tgcttgtaag aggagactta tctttttctt caattttttc agcaactaca ccttctttaa
     4981 aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat attgcaccta
     5041 ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca ccattttttg
     5101 caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa cctatattaa
     5161 caaaagaata ttgtactgaa aatgctgtat caagttcttc ttgactatca aaaagctgtc
     5221 catttaccgc agatacgtta cctttaaaga atgcagtacc aattgaaact aagattatca
     5281 ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct acccccatga
     5341 taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat atatatcccc
     5401 caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata gcagcttgtc
     5461 ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata tatattaagt
     5521 actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag atataaaatc
     5581 ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat cctttcttaa
     5641 ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga tataatatta
     5701 ttattttgta aaacaatttg taattattat taagataatt gcgcatttat ttgtagagta
     5761 catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat tatataaatt
     5821 cactattttc agatatagtt ataaattaat atattctcat ctaataccat gaaaaaacct
     5881 tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa atcccccttt
     5941 tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga ataaagcaac
     6001 atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt acttcaccca
     6061 accaattctc aacttaaata actaaactac tatctattca cagattaggc ttatttctta
     6121 cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt ttaaagtaat
     6181 tacccatata ttcgctttga tactatttat tgaggttata ctctttatgt ataatatatt
     6241 atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa aactttttca
     6301 cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta ccataaatat
     6361 gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattagaa aaattcaaat
     6421 tatatttatc aattatatta tataaaatat ttcctatgaa aatataaatt aaattaatgt
     6481 tcagaatagt ttttagatat gaaatcttga tttttttatt actttactta tttgctcaaa
     6541 tatatttttg tatgcagtat ataataatca aattatacac ttcttataac tgcattgatt
     6601 tgaaaataat attaataatt aatagaataa aaaatttaaa ttaaatttaa gaaaatttgt
     6661 taagttgatt gaatactttt ttatgtattt aattaatttt ataaattcaa ttattttcta
     6721 aatttaggta tgctttacta tataaaaatg attttttatc ttaattttct gaatataagt
     6781 gaatgctatc attttaagta tcttataagc aatgtaacta tatatctcta ataactactt
     6841 tttt
//
LOCUS       DAEMHZ010000171         6773 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000171 DAEMHZ010000000
VERSION     DAEMHZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.172"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..429
                     /locus_tag="KPK72_003444"
     CDS             58..429
                     /locus_tag="KPK72_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490618.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            complement(426..566)
                     /locus_tag="KPK72_003445"
     CDS             complement(426..566)
                     /locus_tag="KPK72_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490619.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(1231..2127)
                     /locus_tag="KPK72_003446"
     CDS             complement(1231..2127)
                     /locus_tag="KPK72_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2490620.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            2268..3695
                     /locus_tag="KPK72_003447"
     CDS             2268..3695
                     /locus_tag="KPK72_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2490621.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3891..6485)
                     /gene="clpB"
                     /locus_tag="KPK72_003448"
     CDS             complement(3891..6485)
                     /gene="clpB"
                     /locus_tag="KPK72_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG2490622.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(6522..>6773)
                     /locus_tag="KPK72_003449"
     CDS             complement(6522..>6773)
                     /locus_tag="KPK72_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490623.1"
                     /translation="ILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFD
                     FFNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
BASE COUNT         2218 a         1060 c          733 g         2762 t
ORIGIN      
        1 attttatttt actttttatc aaaataactt ataatttcgg taaggagtga tgtacatatg
       61 gaaagtttag gagatagaat agcaaatcta cgaaaagaat tagatataaa tcaaaaagaa
      121 cttgctacta aagtcggtat aactgaggct agcttgtcac gttatgaaaa taatttaaga
      181 gaacctaaaa gtgaaataat agttagatta gcaaaagcat tagaaacatc aacagattat
      241 ttattaggcg taaatgataa tacaaaaata agcaaagaag ataaattaat catagagaac
      301 ttatcagtaa gtgaaaaaac aaaaaagctc ttagaaaaaa tatattctct tgaaaaagaa
      361 gatagagaag ctatagaaaa aatgattgat aatgcctatt taaaaagatt ccttaaagaa
      421 gagaattaat tctcttcttt ttctttatac aaaatctcaa gtaataattc ataaaaagta
      481 atatcttcac tcaataaatg ttctaatttc attcctatat cttcaatttt ttcttttttt
      541 gtaacagtag tgtttgactt ttccacatat accatccccc aacaaactta cgttcttaat
      601 gttagtcaga aattcccaac aaatttttaa taaagttcat gtaattgtat tttgatttta
      661 taaaatatat tttgattatc caaaatttcc ctagataatt atcttaatta tataataata
      721 cttttattct acagttgcaa taaaaaactc gtaataataa caaaaaaatc tattattgta
      781 gaatcttgta tgttttttct atttttatat atttttcagg atgattttta ttataaaatt
      841 ttttataaat atataatttt tttatattta tttgactttc ttttaattct ttaggatgga
      901 tttaaatttt ctataaaaat catactattt tagctgttta cttgcttatt taatagtttt
      961 atttctttga ttaaatatct ttcagaatct aagccagatg tctcaaaaaa actgatttat
     1021 aatataatca ttttttctgc tattcttatt tttccaattt tagtttcata ttaaaatata
     1081 cttttcatag acactacatt ctttcatact cattttcaca tttaactata caagtagtct
     1141 tactcattta tatcctatgc tttattaata tttattaaga aaaattcaaa gatttaaaaa
     1201 tatatatttt ctataaagta tatttatgaa ttatttcata tcttctttta aaataacatt
     1261 actaacttct ctatgttcat ttctatactt agttggagat atattataat tctttttaaa
     1321 taagcgacta aaataattta tgctatcaaa gccacatctc atagcaattt ctgtcatatt
     1381 taaatctgtt tcctttaaat aatatatagc tttatcaagc ctgattccat ttataaaatc
     1441 gattgtagtc ttacctgtta tttgcttaaa tgttctacaa aagtgatgtg cactcaagtt
     1501 cacacgacta gcaagttctt ttaatgtaat tttatccata tatttttctt ctatcaaatc
     1561 aaagatacat tcaaattttt ctaaagaata ctttcttttt ataaattcat cttcagaata
     1621 tattttatca atatgacttc ttaataagag tactattaat tgatagatat tagcttttat
     1681 agataactca tatccaactt ctttattgtg atattcatca agtatagttg taatacattt
     1741 taaaatcaga gaatcatttt gaattaaatt attaaataca atatgattta aagccaatgg
     1801 tgctaagtac ttggtttgta acaaatcaac ttgattacta aaaaggaatg tgggttcaat
     1861 acgaatacta tagaatttta aattatctga taagctttct aaataatgta attcattact
     1921 gtttgctatt acaatatttc caggcactac cttaaaatta tccaatccac attcaagata
     1981 tgctatacca tcaataaaat aatatatttg tagatgctca tgccaatgtc ttttaaagtt
     2041 tgctcctttt ttaatgtcat cctttaaaaa aatatctata aagttttctt gacctttcct
     2101 gttaaaatat gtatttttat ccttcattat tcccccctca tcaaaatagt gctaattata
     2161 agcaatatag ttattgtatt cattatttca tatcgctatt atataaaatg taactatcat
     2221 tgtaaacatt aacataatgc agtcattata ataaggaggt aataaaaatg acaaactctt
     2281 ttaaaaaaga tgataataga aaaaaaatca cagttttatt tgtagtcatt gccatgactt
     2341 ttatggcaac attagattct agtattataa atgttgctct cccagttctt gcaagcaaac
     2401 taaatgtatc tctagcatct attgaatggg taatagcaag ttactcaatt attatttgtt
     2461 caacactatt attttttgga agacttggag atattattgg taaatcaaga gtatttcaag
     2521 taggtactat tctctttaca tcagcttcat tactatgtgg attaagtaat tctttaactt
     2581 tacttatagt ttgccgcttt attcaaggaa taggcgcttc tgcctacatg gcaaacaatc
     2641 atggaattat cacagaacta tttccaaaag aaagcagagg aaaagcccta gggattttag
     2701 ttactgcagt tgctattgga aatatggttg gaccatctgt gggaggattt attttgtcaa
     2761 tatttgattg gaatgttata ttttttataa atattccaat cggtcttatt gtaatctttt
     2821 taaatacaaa atttcttcct aatagtaaaa aatcttcaga aaatatggat aaaactggtg
     2881 ctatacttca atttttagga acaacattat tttttagtgc tctaattagt gctcaacaaa
     2941 caggattact aaatccatat atattaattg cactattatt atcaattatt tttattatat
     3001 tatttttgat attagaaaaa aaacatcctc aaccgcttct tgaccttgaa atttttagaa
     3061 attttaaatt ttcattgaat ttaatttgtg ctcttacatc attcatttgt attgcatcat
     3121 caagtatttt aattccattt tatttacaaa gtactatgaa acttccccct attcaagcag
     3181 gtttatttat gatactatca cctttgattt tagccatttt ttcacctatt tttgggaata
     3241 tatcagataa aattaagtca gaaaaaatta ttttaattgg tcttctcgtt atgagttttg
     3301 gattctttct aatgtcaaga ttgaaagaaa gttctgccct tattttattt gtaatttata
     3361 tatcgataat atcaatagga caagctatat ttcaacctgc taacaatgct ttgataatgt
     3421 caagctgttc tagaagtaag ctaggagttg taggaagcat aaattccctt gtaagaaatt
     3481 taggacaagt tataggtatt actatttcta caacactttt atataatttt atgagtatta
     3541 aagctggata tagggtcaat gattatgtga ttaataatga taaaatattt gtatttggaa
     3601 tgagaaatgt ttatattatt gttacacttg tttgcttaat aggtgcaata ttaattggct
     3661 tttatttatt taaatataat aaaaatgaac aatagttttt ataattatat tcaaattatg
     3721 attatattat aagaaattta ctaaaatact tcctttgagt taaatttaat ttggacatat
     3781 tgacttaaaa atgttttatt aatttgataa aatatttctt ttcctatagt ttataaataa
     3841 aaatcagctg agcaaaatat ttagtatatt atgctcaact gattctttta ttatttaact
     3901 aaaacttcaa tattttcatc ttttccatct ataactatag tacatccatt atacacatct
     3961 ccagcaataa gtttctttgc aattatagtt tctaatgtat ttcctatata tctcttaaga
     4021 ggtcttgctc catatacagg gtcatatcct tctctcacca taacatcttt tgctgaattt
     4081 gtaacttcaa tagtaatatc tttttctttt aatctatttt ttaaatcttt cataaatata
     4141 tctataattt tcttgatatt ttcttggtct aaaggcttaa acattataat atcatctact
     4201 ctgtttaaaa actcaggttt aaatctatgc ttcatttcat tcattactaa attgttagta
     4261 gtctctgtta tatttcctcc tgcttctaat agataattac ttcctatatt agaagtcatt
     4321 attataagag tatttttaaa gtctactgtc ttacctttat tatcagtaag tctaccatca
     4381 tctaatatct gtaagaacat gttaaataca tcctcatgtg ctttttctat ctcatcaaat
     4441 agtattacag aataaggagc acgtctaact gcttctgtaa gttgaccacc ctcttcatat
     4501 cccacgtacc ctggaggagg tccaactagt cttgatacag catgtttttc catgtattca
     4561 gacatatcta ttcttattat attatcttca ctatcgaata aatttctagc aagagttttt
     4621 gcaagttcag ttttaccaac acctgtagga cctaagaaaa taaatgaacc aattggtttt
     4681 ctttcatctt ttaatcctgc tcttgcacgt attactgcat ttgaaactgc tgtaactgct
     4741 tcatcttgtc caataactct tttatgaagt tcatcttcta gtttaagtaa tttttctctt
     4801 tctccttcaa ctagtttagt tactggtata cctgtccact ttgatacaat gctagatatt
     4861 tcttcttcag taacttcttc ttttaaaaga gaattttcag aaccatcatt catattttct
     4921 tcgtactgct ttatttgttc ttctaactta ggtatttcac catatttaac ctctgctgct
     4981 ttgttgaaat catattctct ttcatacttt tcagctttac ctttagcttc gtctaattga
     5041 gcctttaagt ttttaatatc taatatttga ctcttttctt tttcatattt tgctgtcatt
     5101 tcatcatttt tagattttaa ctctgctatt tctttttgaa tatcatccaa tcttgattta
     5161 cttttatcat cattttcttt aagtaatgct tctctttcag tttctaatgt aaataacttt
     5221 cttctaacaa catctaattc tgttggcaat gaatctattt cactacgaat catagctcca
     5281 gcttcatcaa ttaaatcaat tgctttatct ggtaaaaatc tatcttgtat atatctatga
     5341 gaaagttttg ctgctgctac aatagcatta tcatgtattc ttactccatg atgtatctca
     5401 aatctttctt ttaaacctct aagtatagat attgtatctt caacagtagg ttcttctgca
     5461 attacaggct gaaaacgtct ttcaagagcc ttatcttttt ctatatactt tctatattca
     5521 tcaaaagtag ttgcaccaat acagttcaat tcacctcttg caagcatagg tttaataaga
     5581 ttacctgcat ccattgaacc ttctgttttg ccagctccaa caatagtatg aatctcatct
     5641 ataaacagta taatttttcc atcagagctt tgaacttctt ttaaaactgc ctttaatctt
     5701 tcttcaaatt cacctctata ttttgcacca gcaattaaag ctcccatatc aagtgagaat
     5761 acaatcttat cttttaaacc ttctgggacg tctcctctta ctattctctc tgctaaccct
     5821 tctacaattg cagttttacc tacacctggc tcaccaatca atacaggatt attttttgtt
     5881 cttcttgata aaattcttat aatccttctt atttcttcat ctctcccaat tacagggtct
     5941 aatttatttt tcttggctaa atctactaag tttgttccat atctagcaag tgcatcatat
     6001 gtgccttctg ggtcttgtgt ttctactctt tgactacctc tcacttgtga taaaatattt
     6061 aaaaagtcat tcttatttat attatattgt tttaatattt taccaacagc tgattttgat
     6121 tctgtttcca tcattgcaag cataacatgc tctacactta tatatgaatc tttaaattct
     6181 ttagaaatac tctctgcctt tataagcacc tcatttattt ttctagtagc tgatactcct
     6241 tgtgaatcag caccttctcc atatactttt ggaagcttat caatttcaaa gtttacagta
     6301 tttctcaaag aatcaattga tatattcatt ttttcgatta tatttggtat caatccatcc
     6361 tcttgattaa taagtgctga gaaaagatga attacatcta cttgttggtt atgatttttt
     6421 actgcttcgt tataagcctc atttaaactt ttttgaactc ttaaagtcat tttttctacg
     6481 tccatataaa tccctccatt ttaatattat atccaaataa attaaattct ctcatttata
     6541 attattgatt ttctaagctc taaaagacta tcaagtaaat tctcgttttt ttgtatatcg
     6601 aaactcaatg ttaaattaaa tgttccatct gaattaaaaa aatcgaatac ttcttttgat
     6661 tttgctatta aatacaagtc ttcatctata agtctttttt cacctattaa taaatactga
     6721 catttatcta taaaactctt tttaaaatca atagtgtata gattttctaa aat
//
LOCUS       DAEMHZ010000172         6723 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000172 DAEMHZ010000000
VERSION     DAEMHZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.173"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            522..791
                     /locus_tag="KPK72_003450"
     CDS             522..791
                     /locus_tag="KPK72_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2490624.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1009..1254)
                     /locus_tag="KPK72_003451"
     CDS             complement(1009..1254)
                     /locus_tag="KPK72_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG2490625.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCTVYFASTENPEPEKLKWWYWKD"
     gene            1672..2370
                     /gene="tcdC"
                     /locus_tag="KPK72_003452"
     CDS             1672..2370
                     /gene="tcdC"
                     /locus_tag="KPK72_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG2490626.1"
                     /translation="MFSKKNEGNEFSNERKGSSKKIIKFFKSTKDIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GKITIPGIAVEKIN"
     gene            complement(2705..>6723)
                     /gene="tcdA"
                     /locus_tag="KPK72_003453"
                     /gene_synonym="toxA"
     CDS             complement(2705..>6723)
                     /gene="tcdA"
                     /locus_tag="KPK72_003453"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG2490627.1"
                     /translation="LSKIDINKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLI
                     IEINLVAKSYSLLLSGDKNYLISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAI
                     SKTSQKSIIHYKKDSKNILEFYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDN
                     NTDKSIDFSISLVSKNQVKVNGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNIS
                     FWKLFGFENINFVIDKYFTLVGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSG
                     NGRNVVVEPIYNPDTGEDISTSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYY
                     SNEYYPEIIVLNPNTFHKKVNINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIR
                     IKGILSNTQSFNKMSIDFKDIKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYY
                     YDEDSKLVKGLININNSLLYFDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIING
                     KHFYFNNDGVMQLGVFKGPDGFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDN
                     DSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRY
                     YFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAI
                     VYQSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFN
                     TNTAEAATGWQTIDGKKYYFNTNTSIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGF
                     EYFAPANTDANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNN
                     AIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEY
                     FAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAV
                     AVTGWQTIDGEKYYFNLNTAEAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFN
                     TDGIMQIGVFKGPDGFEYFAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVT
                     GLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDG
                     IMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWA
                     TIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANN
                     IDGQAIRYQNRFLHLLGNIYYFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEID
                     GVIYFFGVDGVKAPGIYG"
BASE COUNT         2377 a          931 c          882 g         2533 t
ORIGIN      
        1 acatagaatt atcaaatata ttgaatgaat atttgactct tggaatgtgt aattaaatta
       61 agtttggtta ttcgatggaa ggcaaatttg atataaaact ccatttaaag tgccagataa
      121 aaactttatg gttatataaa agttaaaagt aacttatagt gttatagaat attcaaagga
      181 atttaaagta atctatggtg ttttgaaata ttcaaaggga tttaaactaa tctatagtct
      241 tgtagaatac tcaaagagat ttaaactaat ctatagtctt gtagaatatt caaagagatt
      301 taaagcaatc tataatgctg taaaatatat tcaaagggat ttgaagtaat ttattgtgtt
      361 ttgaaatatt caaaaatatt tttggaatag tttttataaa tttaccagta ctaataattt
      421 tatgtttaac atttagatga cctaaaatca actatttgca cttttgtatt catctaaaat
      481 cattttataa tatttttaaa taatggaaag gatgtttgaa catgtttgaa tcaagatgtg
      541 gtgtctgttg tgacagttgt acaaaaaaag aacaagtaaa ttgcactggt tgcccaacta
      601 tggaaaaacc attttggggt ggagaatgta aggttaaaac ttgctgtgaa agtaaggaac
      661 ttaatcattg tggtgaatgt gatactttcc cttgtgatat gcttttaaat aagggaaaag
      721 accaaggttt tgaccctatg gtaaatattg agcaatgtag aaaatggctt gaagaaacag
      781 tttctaatta attattttag aatgctttgt taagtaattt atttaatcct ttgttaggta
      841 gtatgtttag tcatttgttg attggtatat ttatacattt attgatgggt atatttagcc
      901 atacaaatag atttacataa aattcacgac taaaaaagag ttgagtatta gtttttatta
      961 tttaaaacgg atattcaact cttttttatg tctaactttt ttgatttttt aatctttcca
     1021 ataccaccac tttaattttt ctggttctgg attttcagtt gatgcaaaat atactgtaca
     1081 tctgtataca tataactgac atctgtctat gtgctgtcca cgcattaaac attccttttc
     1141 atacatttgt gctgggtttg catctttaag tgatttaata gttgtatatc ctaacatgat
     1201 taaagatttt tctgttgctt tccctacccc tggaattttt tgtagttctc ccatacttca
     1261 ccttctttct gatatattat ttttgtatta tacttagtac cagatatttt tttattatag
     1321 ttaatattta attttttatt atatcacttt atttatgctc tttcatctat ctatatttta
     1381 ccacctctaa agtactgaat catttaatta catcataata tagttttata caaataaaat
     1441 actttatgtt tcatttaata tataaaattc accttcaaga aaattatatt ataatctgac
     1501 atttttacct catttttcaa aatatatcga atcttcttga tttattttgt aaaattatgc
     1561 ttaggggaaa tatattttag gaaaatatga atatataatt tttagtcaac tagttatttt
     1621 aagtttttaa attttaaaat aaaatatatc taataaaagg gagattgtat tatgttttct
     1681 aaaaaaaatg agggtaacga atttagtaat gaaagaaaag gaagctctaa gaaaataatt
     1741 aaattcttta agagcacaaa ggatatcgct ctactggcat ttattttggg cgtgtttttt
     1801 ggcaatatat cctcaccagc ttgttctgaa gaccatgagg aggtcatttc taatcaaaca
     1861 tcagttatag attctcaaaa aacagaaata gaaactttaa atagcaaatt gtctgatgct
     1921 gaaccatggt tcaaaatgaa agacgacgaa aagaaagcta ttgaagctga aaatcaacgt
     1981 aaagctgaag aagctaaaaa ggctgaagaa gctaaaaagg ctgaagaaca acgtaaaaaa
     2041 gaagaagaag agaagaaagg atatgatact ggtattactt atgaccaatt agctagaaca
     2101 cctgatgatt ataagtacaa aaaggtaaaa tttgaaggta aggttattca agttattgaa
     2161 gatggtgatg aggtgcaaat aagattagct gtgtctggaa attatgataa ggtcgtacta
     2221 tgtagttata aaaaatcaat aactccttca agagtgttag aggatgatta cataactata
     2281 agaggtataa gtgctggaac tataacttat gaatcaacta tgggtggaaa aataaccata
     2341 ccagggatag ctgtagagaa aattaattaa tattgttttg tattatagtt aatattttat
     2401 attatagtca atatgtttaa agatgttttt ataattgcaa ataaacagtt acaaggctct
     2461 aaattagttt ttgcttttag catattatct attttctatc aactattaat tatttagtat
     2521 taatatttcc atatatgaat tttattataa aatagtcaag aataatagat tattaaatga
     2581 tagaaaaatt ttaactaaaa gtcatgtatt acaataacac atgactttta attaaatctc
     2641 aatatttatt atataaaaat aatttctgag tagcacagga ataatttttt atcaaacata
     2701 tattttagcc atatatccca ggggctttta ctccatcaac accaaagaaa tatataacac
     2761 catcaatctc gaaaagtcca ccagctgcag ccatagcagt atcaggcata aagtaatata
     2821 ctttaccatt aatagtttgc catccagtaa ctgcttttga attattacca aagtaatata
     2881 tattgccaag taaatgtagg aatctatttt gataacgtat agcttgaccg tctatattgt
     2941 tagcatctgt attagcaggt gcaaagtatt caaatccatt aggtccttta aacaccccta
     3001 tctgaggtaa accatttcta aagtaaaaat ttttattatc aatagtttta taaccattcg
     3061 cacccatagc tgtattaggc tcgaagtaat atctattacc atcaatagtt gcccaaccag
     3121 tagccgcttt tgaatcattg ccaaagtaat atatattgtc atgtaaatat aggaatctat
     3181 tttgataacg tatagcttga ccttctatgt tgttagcatc cgtattagca ggtgcaaagt
     3241 attcaaatcc atcaggtcct ttaaacactc ctatctgcat aataccatca gtattaaaat
     3301 aaaaatgttt accactaata attgtataac cagttgaagc tatataagtg ttagtattaa
     3361 agtagtattt tttaccatta atagtttgcc atccagtaac tgcaacagca gtgttagtat
     3421 taaagtaata ttttttacca tcaatagttc gcaatccggt aactgctttt gagtcactac
     3481 caaagtaata ttttttacca ttcaaagtta agaatttatt ttggtaaagt atagcttgac
     3541 cttctatgtt attattatga gtattagcag gtgcaaagta ttcaaatcca tcaggtcctt
     3601 taaacactcc tatctgcata ataccatcag tattaaaata aaaatgttta ccattaataa
     3661 tcgtataacc agttgaagct atataagtgt tagtattaaa gtagtatctt ttaccatcaa
     3721 tagtttgcca tccagtagct gcttcagcag tgttaagatt aaagtaatat ttttcaccat
     3781 caatagtttg ccatccagta actgcaacag cagtgttaag attaaagtaa tattttttac
     3841 tatcaatagt ttgccatcca gtaactgctt ttgagtcatt atcaaaataa tattttttgc
     3901 cattcaaagt taagaattta ttctggtaaa ctatagcctg accttctatg ttattattat
     3961 gagtattagc aggtgcaaaa tactcaaatc cattaggtcc tttaaatact cctgttacca
     4021 ttttagattc attattagca tcaaaataga aattatttct ttcaatagta atatatccat
     4081 tttgaagaat tccatcataa ctaaagtaat acttgtcgtt atttatagtg catagatgaa
     4141 ttgcagcaat agcattatta ggattaaagt aatatttctt attgttaata attctccatc
     4201 cagtaactgc ttttgagtca ctaccaaagt aatatttttt accattcaaa gttaagaatt
     4261 cattttggta aagtatagct tgaccttcta tgttgttagc atccgtatta gcaggtgcaa
     4321 aatattcaaa tccattaggt cctttaaaca ctcctatctg cataatacca tcagtattaa
     4381 aataaaaatg tttaccatta ataattgtat aaccagttga agctatagaa gtgttagtat
     4441 taaagtaata ttttttacca tcaatagttt gccatccagt agctgcttca gcagtgttag
     4501 tattaaagta atacttttta ccatcaatag tttgccatcc agtagctgct tcagcagtgt
     4561 tagtattaaa gtaatatttt ttactatcaa tagtttgcca tccggtaact gcttttgagt
     4621 tattatcaaa gtaatatttt ttaccattca aagttaagaa tttactttga taaactatag
     4681 cctgaccttc tatgttatta ttataagtat tagcaggtgc gaaatattca aatccattag
     4741 agccactaaa cacacctatt ttcactacac aatcactatc aaaataaaag tgtttaccat
     4801 caatagtttt ataaccatta aaggcaatag cggtatcagt atcaaagtag tatctactac
     4861 cattaacagt ctgccaacct tttgagatga tagcagtgtc aggattgaaa taatacttat
     4921 tattgtcaat tacttgcaat ccgactgcag caatagcatt attaggatta aagtaatatt
     4981 tctcattgtt aataattctc catccagtga ctgcttttga gtcattatca aaataatatt
     5041 ttttgccatt caaagttaag aatttacttt gataaactat agcctgacct tctatgttat
     5101 tattctgagt attggcaggt gcaaaatact caaatccatc aggtccttta aatactccta
     5161 actgcatcac accatcatta ttaaaataaa agtgtttacc attaataatt ttataactag
     5221 ttgaagctgc tccagtattt atatcaaaat aatatttttt accattgata gtttgccatc
     5281 cagttactaa gttagattct ataggatcaa aatataataa tgaattattt atattgatta
     5341 atcctttaac taatttacta tcttcatcat agtaataagt tttattatct attattttaa
     5401 atcctaaatg atctctatct aattcatttt cagaattaaa tgatttaaaa ttactcatta
     5461 tatatcctaa tgatagtttt ttaatatctt taaaatctat actcatttta ttaaatgatt
     5521 gagtattaga taagatacct ttgattctta ttttttgtaa tattttttta ttactttctt
     5581 ctaagtatct aactaaaata aagtcacttc cttctgtaga ccatttatac tcaaaagaag
     5641 aactatctaa atttatattt acttttttgt ggaatgtatt tgggttaaga actataatct
     5701 cagggtagta ctcatttgaa taataattgg tattaatatt tattaaactt gtatataaat
     5761 caggtgctat caatacttta ttgatatatc tatctattcc atagagaggt tcataggaaa
     5821 aatctagtga agtagatata tcttcacccg tatcaggatt atatataggc tctactacaa
     5881 catttctacc atttccgcta aatatagtgc ttttagatga cgatgttttc cattcaccaa
     5941 aatatatatc tatattttta ttattgtcac aaataaattc tacatatcca agattagttt
     6001 taccaacaag ggtaaagtat ttatcgatta caaaatttat attttcaaac ccaaacaatt
     6061 tccagaaact tatattgttc aaaaataaat tcataaaatt agaagtatta tggtgtccat
     6121 ctgaattttt cacaaaatca aggtaagatg agtatacgga ttcatttaaa tataatccat
     6181 ttacttttac ttgattttta ctaactaaag aaatagagaa atctatactt ttatcagtat
     6241 tattatcaac atagtatttt cctgttatag tattaatatc atctttcata aatacattta
     6301 tatcttcagc aataaaatct ttactgttaa attctaatgt actgccatta taaaattcta
     6361 atatattttt actgtctttt ttataatgta ttatgctttt ttgacttgtt ttagatatag
     6421 ctccaaaata tttattatta gattcatcag tgtaattgta cgctatattt ttactatcta
     6481 ggcctaaagt attgattttc tcaatagtat tagataaatt ggatatcaaa taatttttat
     6541 ccccagacaa taacaaacta taagattttg caacaagatt tatttctatt attaaactaa
     6601 ttttatcatc taactcacaa gtcaagaata tatatctatc tttgttatct atatcgcctg
     6661 aaaaatctat tgtttgattg cctataataa gtttattttt atttatatca attttactta
     6721 gaa
//
LOCUS       DAEMHZ010000173         6607 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000173 DAEMHZ010000000
VERSION     DAEMHZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.174"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            310..2322
                     /locus_tag="KPK72_003454"
     CDS             310..2322
                     /locus_tag="KPK72_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490628.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2691..3911
                     /locus_tag="KPK72_003455"
     CDS             2691..3911
                     /locus_tag="KPK72_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2490629.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHISDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEKKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3948..5297
                     /locus_tag="KPK72_003456"
     CDS             3948..5297
                     /locus_tag="KPK72_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2490630.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGSYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(5429..5722)
                     /locus_tag="KPK72_003457"
     CDS             complement(5429..5722)
                     /locus_tag="KPK72_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490631.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(6208..6411)
                     /locus_tag="KPK72_003458"
     CDS             complement(6208..6411)
                     /locus_tag="KPK72_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490632.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2675 a          790 c          892 g         2250 t
ORIGIN      
        1 tattttaata taattctata tttacaaaat tatattatca atagaattca ttaaaataaa
       61 tttttttggg tgtatattat aatttaatcg aatagaaatg aaaaatatac atacagttta
      121 agttttaagc aacaattagg gggatttggt gacaagattt ttaattataa gaaagaaagt
      181 gtcttaatgc aacttttata aagtcattat agacactttc ttttttattt gataaattta
      241 aattatcaaa attatattgc tttatacaac tattttgttg gaaactattt attatataag
      301 gagaaataaa tgaaaaaatc agtagctctg gtaaatgata gtagaaaaga tttaatagac
      361 tttttagaaa ataatttgaa attagttttt ggagattcta taaatattaa tagatacttc
      421 ataaatgaaa taaatgataa tgatattata aatgatgatg tgatattagt aatgtcagta
      481 gaaagattag acaaaattat aaataatata ctagataaga aaaaagtgat tgtggtaaga
      541 agaactttta gggaagataa aatttataat ctactatctt taccacaggg tacaaacgtt
      601 ctaatcgtaa atgattctga tgaaactact ctagaaacta taagtttatt ttataaaatt
      661 ggagtgacta atattagacc aattccatat atgaatgata ataactataa aaatataaaa
      721 atagctataa ctccaggagt tccagaaaaa gtacctagct ttatctcaga tatatttgat
      781 ttaggtcaca gatatataga tatctctaca tttatagaaa taataaattt attacaaatt
      841 gattcaaagg aaatacaatc taatctagtt aaatactcag aagaaataat aagtttagat
      901 actggtataa aagataaata taaggaatta tttttaaaaa tagaagaatt agatacaatc
      961 ttgaatttat caaaagatgg tatattattt acttcaaaag atggtgaaat aaatacatat
     1021 aatagtaaag taaaagatat tttagacata aatgaggata tatatggaaa atacattgag
     1081 gatatatttg tagatagttt aaaagtttta ttgagtgaga aagagatact agataaagtt
     1141 gtcgtattca ataagaaata tataaatgta aataaaaaaa acatatacaa tagagatgaa
     1201 aaaatgggca catattatag tttacaagaa ataacataca taaaaaaatt agaacaaaat
     1261 ctcactaaaa aattaagaga aaaaggacaa atagcaaaat atacatttaa ggatataaaa
     1321 acaaatagtc ctaaaatgtt tgaatgtata gatttagcta agaaggtttc taaatctgac
     1381 ttaagcatac ttatcagagg tgaaagtgga actggcaagg aactcatagc tcagtctata
     1441 cataacaact caaatagaaa aaatcaaccc tttatagcag taaattgtgc agctgttcct
     1501 gaaaatttat tagaaagtca gctattcgga tatgacaaag gaacttttac tggaggtcta
     1561 aaagatggaa aacaaggatt atttgaactt gctaataatg gaacaatatt cttagatgaa
     1621 ataggcgata tgcctttaga attacaaaca aaactactta gagttcttca agagaaacag
     1681 ataatgcctg ttggttctca caatgtgatt aatatagatg ttagaatcat atctgctaca
     1741 aataagaatt tagaacaaat gattgataat agccaattta gagaggattt atattatcga
     1801 ttaaatacaa ttccaataaa tatacctcct ctaagagaaa gaaaagaaga catattaatt
     1861 attatggaag atttaattaa taagaaatta gtaatcacac cagaagcaaa aaaacttata
     1921 caaaactata tgtggaaagg taatataaga gaactccaaa atgttacatc ctatttaaac
     1981 attatgtgtg aagatattgt attagagaaa gatttacctc caaacttaag gtcttcagat
     2041 aacaaaaata catcattaaa actaaaatat agtaagaatg atatattgaa tatattagag
     2101 atactaattt taaataagga atcagatgtt ggtattggta gaggattaat tttaaaagca
     2161 ctactcgata agaaccttca aattacagaa ggaaaaatta aaaaaatatt tgaataccta
     2221 aaaaaagaag aacttataat ttgtagttca ggaagatatg gaagtaaaat aactcaaaaa
     2281 ggagaagatt tttacaataa attaaaatat aaaggtctat aaaattataa tttatcaaac
     2341 aaagaaatct attcaggatt aaagtactcc ttcaatagtt ttttttattt ttcaagtacc
     2401 ctaaaattat aatttatttt gtacataaat aataggaatt aataggaata ggataatttt
     2461 tttatttcct atttttttta tataaactag catataaaat atattttctt caaaataata
     2521 ttttttactg aaaattgaaa ctatgcaatc attttttata ctatacatat agataaatga
     2581 ttatatttat tcaatattct tataaaaaac aaaattttcc atcgaaaatt atggcataat
     2641 aattgcttat tatattaata aaacaataaa aagcaattgg aggaataaaa atgcaacagg
     2701 ctttgaaatt aaaatatcaa accaaagaat taggaaaaga ttttgtaata gaaagttgta
     2761 attctataaa accatggctt ataaacataa gaagagaatt acataaaatt ccagaattag
     2821 ctcttgaaga aaatcttact aaacagaagg taatttctta tttaaaagaa atcggaattg
     2881 attatatgga atttacaaaa cacaatggaa ttatggcata tattttaaaa gaaagtgcag
     2941 ataaaacgat ttgtataaga gcagatatgg atgctcttcc tattgaagaa gaaaacaaca
     3001 taccttataa atcaatacat tctggaaaaa tgcatgcttg tggccatgat gcacatacaa
     3061 ccatgctact tggagcatgt aaagttttac attcaataaa agataaactt aatgtaaatg
     3121 taaaattctt atttcaacca gcagaagaag gttttggagg agcaaaattt ttagttgagg
     3181 atgggtgttt agaaaacccc aaggccgatt atatattcgg acttcatgtt atgccacata
     3241 tagaaacagg ccttatcgaa acaaaatatg atacattaaa tgctagtgta gacactataa
     3301 aaataagtat caaaggtaaa agagctcatg gtgcatatcc tgaaaacggt attgatgcaa
     3361 tagttacagc ttcacaaatt gttacatcat tacaaactat aataagtaga aacttagaac
     3421 caaataatgc tgctgttttg actataggta aaatatatgg tggtgatgca cacaatgtaa
     3481 tatgtgaaga tgtaaaatta gaaggcactt taagaacctt aaatagcaaa acaagaaact
     3541 ttatgattga taaaatagct aaaattgttg gagatactgc atctgcattt ggttgtgttg
     3601 ggactttaca tataagtgat gaaaactatc ctgctgttat aaatgaaaaa gaattagtag
     3661 atacagttat atcaagtact aaagaattac ttggagagga aaaatttata ttgagaccaa
     3721 acccgtcttt aggtggtgaa gacttttctt tttatacaga acattgtaaa ggagcattct
     3781 tccatttagg atgtaaaaat gagaaaaagg gcttaatttc acctcttcat acctctagct
     3841 ttaatataga tgaagattgt ttaccaatag gtgttatgat gcatgtaatg aatactttgt
     3901 attttaatta gtacagtaat taataaaaga ataggagata aattaatatg gaaaaagcta
     3961 agaaattagg tctatttagt atgatattat tagggattaa tagcataata ggttcaggta
     4021 tattcttact accaggtaaa gtttataatc tagcaggtca aaacagtatg tttatataca
     4081 tttttgctac actcttagtt ctatcaatac ttttatgttt tgcagaagtt ggaagcatgt
     4141 ttgataagaa tggaggagca tatctatact ccaaaaaagc ttttggagat tttataggat
     4201 ttgaagttgg tacaatgtct tgggttatta gaattatatc atggtctact ctagcagtag
     4261 gatttgcaac agctttaggt tcattttggc cagaatctgc cactgaatac aaaggttata
     4321 tagctgcaat actcgtaaca ctactttcaa taaatagctt atttggaatt aaaagcacaa
     4381 agataatgaa taatgtaata actatagcta aattagtacc attaatagtt tttataatag
     4441 ttggaatatt ctttataaaa tttgtaaata ttgttccttc aggaaatgta gttaactcta
     4501 gtatgggtcc agccattata ttagtatttt acgcatttac cggctttgaa tcatttattg
     4561 tggcaagtgg agaaatggaa aatcctaaaa aaaatcttcc agtcgcatta ataaccacaa
     4621 tatttatatg tgcaataatt tatatattaa ttcaaattgt ctgtatgggt atacttggag
     4681 atagactatt tgaaaacagt attccaattg cagatgcttc aagtgtattt ttaggcagtt
     4741 atggtaaagt tttcatatct gtagcaacat taatatctat atttggtata aatataggtt
     4801 catcaatagt aactcctaaa tgtggttctt ctctagcaga agaaggttct cttcctgcat
     4861 ttataggaaa aacaaataaa tatggtgctc catatgtagc aataataata tctttaattt
     4921 gttgtatacc acttgtgcta actggaagtt ttgaacaact tgcagtaatg agtgtaattg
     4981 caagatttgc tcagtacata ccaacttgtt tatcagtaat agttttaaga aaaagaacag
     5041 atgttaaggc ctcttttaag attccatttg gtcctgttat accaatggta gctattttag
     5101 gtagcttatg gttgttacaa caagcttggg cagaagatat ttcaaaacct ataactcaaa
     5161 atagagtttt aataggatta ggggcaatgt tgctaatagc tcctctatat atatttatga
     5221 aaaaaaataa agaaatcgag cataaaaata aatcagaaga ttctaaatta tatatttcga
     5281 gagaaaaaat tcaataatat tttatactat tacttctcat aaaatgtaat aaatataaaa
     5341 aaattgtaga taaaactatt ttataaaatt tatctacaat ttttttatat aaacatatat
     5401 caaaatcaaa atattattaa tttatacttt aaagattccc atctttatca tattttattc
     5461 ttataagaaa atataatcct attactacta actctatcac taaaaatact ccataggtaa
     5521 gaattgaatc aatactatct aacccatttc taaaaaaaat taatgctata cctgctgcta
     5581 taatccctat agcatttttt cttactgcat tttgcccaac tccataaaaa cctggcattt
     5641 ttccttttat ttcgacagca gttaaaatta tcccaaacac tataaataat actccaataa
     5701 gcatcataat catactaatc atctaattac ctccttcact taaggaaatt tcaatttcta
     5761 aaatctaaaa taaataatat ttatttttta taataatatt gtatatccaa ttaactattt
     5821 tggaaattag taccaataaa taatgtttaa atattgaaat aatatattaa ttctaatcaa
     5881 tattattaat atatatatac atttttacaa atatatttaa aaactttact tttaattctt
     5941 actatttaaa taagtataat ttattataat aacttttaat taatttatac ttttaaattg
     6001 taaaaatcac ccctaatcac tatatttacc actataataa ttgaagaggt gatttatata
     6061 acagatacta tttatgcagt tattataaaa tcacactctt aattataatc aactgcatat
     6121 ttttataata aaaggaaata aagtaagaaa gataaattta atcaaatatg ctaaatcaat
     6181 ttaatagtat acttattact gaaatattta cattccatca ggagttcttt ggccatgtat
     6241 caatttatta taatatgtat tatatgatcc tcctcttgca ttataatcat tattcttgca
     6301 gttacaagat gaagatgcca tcatcttaca agtataagga cacataatag tacaaacacc
     6361 tcttgtctca acacctatat tattcatttc tattcttttt ccaaaactca ttcatatcct
     6421 ccccttttct ataagatcac ttactttata cctttattaa cctataccat attgattatt
     6481 tttagaacat tatcaaaact cgtaaattgc ctattcaaaa gtttttcctc tggaattttt
     6541 atattaaagt tttctattac ctcgcaaaat actaaaagta attctcttgg ctgtatatgt
     6601 aaagacg
//
LOCUS       DAEMHZ010000174         6379 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000174 DAEMHZ010000000
VERSION     DAEMHZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.175"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            264..1208
                     /locus_tag="KPK72_003459"
     CDS             264..1208
                     /locus_tag="KPK72_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG2490633.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1230..1856
                     /locus_tag="KPK72_003460"
     CDS             1230..1856
                     /locus_tag="KPK72_003460"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG2490634.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1890..2939
                     /gene="hisC"
                     /locus_tag="KPK72_003461"
     CDS             1890..2939
                     /gene="hisC"
                     /locus_tag="KPK72_003461"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2490635.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2942..3520
                     /gene="hisB"
                     /locus_tag="KPK72_003462"
     CDS             2942..3520
                     /gene="hisB"
                     /locus_tag="KPK72_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG2490636.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3522..4127
                     /gene="hisH"
                     /locus_tag="KPK72_003463"
     CDS             3522..4127
                     /gene="hisH"
                     /locus_tag="KPK72_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG2490637.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4124..4846
                     /gene="hisA"
                     /locus_tag="KPK72_003464"
     CDS             4124..4846
                     /gene="hisA"
                     /locus_tag="KPK72_003464"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG2490638.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4828..5586
                     /gene="hisF"
                     /locus_tag="KPK72_003465"
     CDS             4828..5586
                     /gene="hisF"
                     /locus_tag="KPK72_003465"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG2490639.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5614..6282
                     /locus_tag="KPK72_003466"
     CDS             5614..6282
                     /locus_tag="KPK72_003466"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG2490640.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2602 a          540 c         1260 g         1977 t
ORIGIN      
        1 tgggaggaaa ttatcatggt gagattaaat atagtaaaat taaatacaaa attaaatcat
       61 caccatcatc atagattaaa gggattgtaa tatgaaatta attcatagac acaagccctg
      121 tttttagtgt aattggtctt gtatctatga tgggaagtaa ttttaggaga aaattatagc
      181 catataggta taaagcctat atggcttttt tattgtttta aatttgcaaa tataagagta
      241 aatttaatga agagggtgag aatgtgaaat ttttgaggat tgaagatgaa gttatttatt
      301 ctaaaaaaag atataaatta aaaagggaaa tagataaact ttttttggat gaagaatatt
      361 ttcaaataga accacaatta tttgaagaat atgatgaatt tacaactata aataataaaa
      421 ttccagaaga atctatggta aaggttgtaa atggaaaagt aatggtactt agagctgata
      481 taactacaag tataataaaa agccttgtac caagatggga agatggttta aaacttaagc
      541 ttttttataa ttcatcaata tataaaaata aaaatacagt tggaataaaa gagataagac
      601 aaataggttg cgagtatcta ggagaagcct ctgtagaagc agatagagaa gtagtgaaat
      661 tagctcttaa gatacttgaa aaatacaata ataattttat tttagaggtt gggagtagca
      721 agtatattca tggtttgtta gaagaattaa atttaaataa gaattgtgaa aatcaaatta
      781 aaaatttact atatacgaag aacactcatg aattaaaagt atatattgaa gaactgaaaa
      841 ttaagcgtga agtaaaagag ctattatcaa atattttaag cttgcaaggg aatttataca
      901 acgttattga aaaatcaaac aagttctatt gtaataataa gatgaagcaa gctcttgaag
      961 aattaaagca agtaaataat ttaattgagg aatgtaattt tttagataag gctagatttg
     1021 atttatcaat gattactatg cttgactatt atgaaggaat tatgtttaga ggatactacc
     1081 caaactcata taaagaaatt cttagtggtg gaagatatga ttctttaact aaagagtatg
     1141 gaaaagaaat tccagccatt ggatttacac taagtgttga tgagttaatg aagtatgtat
     1201 ataagtaaag tttagaagga gggattgctt tggattattt gactatagca ctagcaaaag
     1261 gacgaattga aggggaatca tttaaaaaat ttaaaaaaat gggtctagga gatagtatag
     1321 acacagatac gagaaaacta atttttaaag atgaagaaaa taaaattata tatatacatg
     1381 taaagccatc agatgtagtt acatatgttg aaaaaggagt cgctgacctt ggtatagcag
     1441 gtaaagatac tattttagag aatgagacag atgtatatga aatatatgat ttaggttttg
     1501 gaaaatgtaa atttgctgta gcaggattaa agggagatag catatataga gaagatgaat
     1561 atctaaaagt ggcaacaaag tatcccaata tagcaaaaaa gtattttaaa gagaaaggtc
     1621 aaaaaattga aataataaaa ttaaatggtt ctgtagaact agctccaata gtagggttgt
     1681 cagatgtaat tgtagatata gttgagactg gcaatacact taaagcaaat ggtcttgaaa
     1741 tattagaaga catatgtaat ataagtgcta gaattatatc aaatagagct agctatagat
     1801 ttaaatatga acaaattcag aatataattc gtttatttga agaacttgat aattaaaata
     1861 taattgagct ttataaggag ggatttaagt tgagagaaaa agaaagcata agagagttaa
     1921 ggggatatga accaaatcac gtaaactgta aagtaaaact tgatgcaaat gaaggaagta
     1981 agaggttgtt taaatacctt attaaagaaa tttcagatag tgatatagat ttaaatttat
     2041 atcctgaaga ttcttatagt gatttgaaag aatctataat agattacata aatatttctg
     2101 gagtaaataa gaaaaatctt ctagtaggaa acggttcaag tgaaataatt gatttaataa
     2161 tacatacttt tgtagataaa gatgaagtaa tattgagttt ctctccaagt tttagtatgt
     2221 attctatata tagtcagata aatgggtcta aatttatagg tgttgaaagt gatgaaaatt
     2281 tagttataaa tatagataat gtgattgaaa aagtaaagga gaataatcct aaaatagtaa
     2341 ttgtatgtaa tcctaataat ccaactggaa caatattaaa gagggaagag ataataaagc
     2401 ttttagattc aactaatagt ctagtggttt tagatgaagc atatatggat tttggagaag
     2461 aaagcatgtt aagtgatgtt tttaagtatg ataatctaat agttcttaga actttatcaa
     2521 aggcatttgg attggctgga ataagaacag gatatatgtt atctaatagt agtttaataa
     2581 attctgttga gaaagtaaga cctccatata atttaaattc attatcagac tttattgcta
     2641 caagagcact tagaaataaa gatgtggtaa aggcttatat aaaggaagta aaagaggaaa
     2701 gagaagtttt atatgaagaa atgattggta tgggaattaa agcatacaaa tcacaagcaa
     2761 attttatatt attttattct gaaatcgaaa atttatcaca aaaacttatt gatagaggag
     2821 ttttaataag aaaatttggt ggaaagttag aaaattatta tagagttaca attggtgata
     2881 aagaagaaaa ttctatgttt gttggagcta taagagatat tttgaaaaag gagaaatagg
     2941 tatgagaatt tggaaggtag aaagaaatac tcttgaaaca cagattttag tagaattaaa
     3001 tattgatggt tctggtaaag ctgaaataga tactggaata ggatttttag accatatgct
     3061 taccttaatg tcatttcatg gcaagttcga tttaaaagtg atttgtaagg gtgatacata
     3121 tgtagatgac caccatagtg tagaagatat aggtatagct ataggtgaag catttaaaaa
     3181 tgctctaggt gacaaaaaag gaatacgaag atattctaat atctatatac ctatggatga
     3241 atctctatca atggtagcaa tagatataag caatagacct tatcttgtat ttaatgctaa
     3301 atttgatacc caaatgattg gtagtatgag tactcaatgc tttaaagaat tttttagagc
     3361 ttttgtaaat gaatcgagag taacattaca tatcaatctc ttatatgggg aaaatgacca
     3421 tcataagata gaaagtattt ttaaagcatt tgcaagagct ttaaaagagg gtagtgaaat
     3481 agtttctaat gagatagcat cttcaaaggg ggttttgtag aatgaatata atagtagact
     3541 atggtcttgg aaatatagat tctgtgtcta ggggttttag aaaagctgga atagaaacta
     3601 aaatttctag tgatatagat gaaataaaac aggcagattc acttatactt ccaggtgttg
     3661 gagcttttag agattctata agtgccttag ataaactagg attgatacca ataataaagg
     3721 agcatgtttc taagggtaaa tttatgatag gtatatgtct aggaatgcaa ttactttatg
     3781 aaaaaagtta tgagtatgga gaatatgaag gattaggact tataaaagga agtattgata
     3841 aactggatat aagtttaaaa gtgccacata tgggatggaa taatctaaaa tttaataagg
     3901 caaatgatga tatacttaaa tatataaatg aagatgatta tgtatacttc gttcactcat
     3961 attatgcaaa ttcatcaaat gaagaactaa ttgcattttc agaatatgaa aagaaaatcc
     4021 ctgctattgt tagaaaaggc aatgtatatg gtatacaatt tcatcctgaa aaaagtggag
     4081 aagtagggtt aaatatatta agagcatatg gggagatgat aaaatgataa tatttccagc
     4141 aatagatata aaagataata agtgtgtaag actcacacaa ggagaatttg acaaagtaaa
     4201 tgtatattat gataatccat tagaagtagc atataaatgg aaaaatgaag gagcagaata
     4261 tatacatata gtagatttaa atggtgctag aagtgaattt ggtgtaaata caaaaataat
     4321 tgaggacata gcaaacaata tagatatacc tattcaagtt ggcggtggag ttagggataa
     4381 ggaaaaagtc aaaagtttaa taaatgctgg tgttactaga gtaatattag gcagtatagc
     4441 gattgaaaat ttgaatttag ttgaagaatt ggtcaatgaa tataaagaga aaattgtagt
     4501 ttctatagat gctaaagatg gaaaagtagc agttagggga tgggaagttg taagcaatgt
     4561 agactcatta acattatgta aacaacttga aaagataggt gtacaaacta ttgtttatac
     4621 agatatatca aaagatggga tgttgcaagg tccaaatttt gacatatatg aaagaatagc
     4681 aaaggaaaca tcactaaatg ttattgcttc tggaggtgta acttctatag aagacgtaaa
     4741 aagactaaaa gctatgaact tatatggtgc aataataggt aaagcgcttt atgataaaaa
     4801 gatagacttt aaggaggcac aacaattatg cttactagga gaataatacc ttgtctagat
     4861 gtgagaaatg gaagagttgt aaaggggaaa aaatttaaag atatagtaga tgttgatagt
     4921 ccagaagtac ttggaaaatt ttatagtgat tgtggtgcag atgaacttgt attctatgat
     4981 ataactgcat caaatgaaga gagaaagaca tctttagaat ttgtgacaaa agttgcagaa
     5041 aatataaata taccattttg tgttggtgga ggagtaaata aattagaaga tttcactgat
     5101 attttaagaa aaggtgctga taaagtttct ataaattcat cagcagtcaa aaatcctgaa
     5161 ttaataaggg aagcttcatt aaaatttgga gcacaatgtg ttgttttatc gatagatgca
     5221 aagaaaaatg aagagggttc atggagcgta tacgtaaaag gtggaagaga aaaaactaat
     5281 ctggatgcaa ttgaatgggc tgttaaaggt gtagaacttg gagcaggaga aatcgttgta
     5341 aatagtatgg atgaagatgg aatgaaaaat ggatatgata tagaattatt atcaaagata
     5401 acttctttag taaatgtacc tgttatagca tctggaggcg ctggaaaaaa ggaagatttt
     5461 tatgaggcgg ttaataaatc caatgtagat ggaattttag cagcatctgt ttttcatttt
     5521 ggcgagataa aaataaacga tttaaagaaa tatttaaaag acatgggtgt agaagtaaga
     5581 ttataagtta aatactaaaa ggagattaga aatatggata ataagtgtaa caatgtatac
     5641 agtgatgaag tagagaaatt tataagaagt ataaagtttg atgataaagg gttagtccct
     5701 gttgttgttc aagaggtagt tagcaaagat gtactgatgt tagcatatat gaataaagat
     5761 gctataaaga aaaccttaaa agataaagta gcttgctatt ttagtagaag tagacaagaa
     5821 ctttgggtta aaggtgagac atctggtaat actcaaaaag tagttaagat gtcttatgat
     5881 tgtgatgtag atactatact actttttgtt gaacaaactg gtgtagcttg tcatacaggc
     5941 aattattctt gtttttacag agacttgttt gatgatagtt caaaaatgga gcttgaagta
     6001 caaacaaata tattgaaaga attatatgat ttaatcaatg aaagaaaaaa taatccagtt
     6061 gaaggttctt atactaatta tctttttgaa aaaggaatag ataagattct taaaaaagta
     6121 ggtgaagaga gtagtgaggt aattatagca tcaaaaaata ccgataaaag tgaacttatt
     6181 tacgaaatta gcgatttagt atatcataca ttggttttaa tgatagaaaa aggtgttgaa
     6241 attgatgaaa taaaaaaaga attacttaaa agaagaaaat agaataaggt agattaatgg
     6301 gtaataagaa aatataccac ttttaataaa aagtggtata ttttttattt tatattttaa
     6361 tttatagaat aatatttaa
//
LOCUS       DAEMHZ010000175         6244 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000175 DAEMHZ010000000
VERSION     DAEMHZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.176"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1029
                     /locus_tag="KPK72_003467"
     CDS             <1..1029
                     /locus_tag="KPK72_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2490641.1"
                     /translation="VSDELDMTSNKVNYVSDELDITSNKTDYISNELYNLSNKSIKGN
                     IPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLE
                     SEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKV
                     IEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPV
                     YISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDI
                     VILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNES
                     MVEIIRQN"
     gene            1117..1452
                     /gene="spoIIAA"
                     /locus_tag="KPK72_003468"
     CDS             1117..1452
                     /gene="spoIIAA"
                     /locus_tag="KPK72_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG2490642.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            1466..1894
                     /locus_tag="KPK72_003469"
     CDS             1466..1894
                     /locus_tag="KPK72_003469"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG2490643.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            1917..2681
                     /gene="sigF"
                     /locus_tag="KPK72_003470"
     CDS             1917..2681
                     /gene="sigF"
                     /locus_tag="KPK72_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG2490644.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            2961..3398
                     /gene="spoVAC"
                     /locus_tag="KPK72_003471"
     CDS             2961..3398
                     /gene="spoVAC"
                     /locus_tag="KPK72_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG2490645.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            3565..4578
                     /gene="spoVAD"
                     /locus_tag="KPK72_003472"
     CDS             3565..4578
                     /gene="spoVAD"
                     /locus_tag="KPK72_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG2490646.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            4592..4954
                     /gene="spoVAE"
                     /locus_tag="KPK72_003473"
     CDS             4592..4954
                     /gene="spoVAE"
                     /locus_tag="KPK72_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG2490647.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            5137..6153
                     /locus_tag="KPK72_003474"
     CDS             5137..6153
                     /locus_tag="KPK72_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2490648.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         2502 a          645 c         1154 g         1943 t
ORIGIN      
        1 gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtatcaga tgaacttgat
       61 ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc taataagtca
      121 atcaaaggta atattcctat aaagatggct gtaataacta aaagtaatat gctgaatcaa
      181 ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttgaatttaa agtatgtttt
      241 gatatgttta taactgatac agctacttta tgtgacatgt ttataccgtg tacaaataca
      301 ctggagagtg aagatataat ttatagctca atgaccaatc catatataac ctataatgaa
      361 agagctgtaa agccagcaca taaattgatg gatgaatatt acttctttat ggagctagct
      421 aaaaagatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt agaaaaagtg
      481 attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa aaacaattac
      541 tttactatac ataaccctgt tgcttgggaa gataagaagt ttgagactcc atctggaaaa
      601 tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc tgtatatata
      661 agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt aactaatcat
      721 catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc tcaagcatat
      781 ataaatcgga gaatggctaa aaaagtaggt attgaggaca aagatattgt gatattgagg
      841 tcaaaaaaag caaaaataaa tgtacaaata aatatagatg atggagtagg taattatata
      901 gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt aacagacact
      961 ggtatatcag attttggggg tcaggtaaca tataatgaaa gtatggtaga aataatacga
     1021 caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg attaaaagtt
     1081 actattttta caaaactatt gaaggaggga tttttgatgg taaattattc cttagaacat
     1141 aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc caatgaaata
     1201 agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt atttaatttt
     1261 gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag atataaaaag
     1321 atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt taagaagatt
     1381 tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga agaagctcta
     1441 agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat tttcggcaat
     1501 atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta agttagaccc
     1561 aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta cgaatgctat
     1621 aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg agatagaagg
     1681 aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg tggagaaggc
     1741 aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg gattcacagt
     1801 aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg ggactagaat
     1861 tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt tttattatgg
     1921 aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag actctagaac
     1981 ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata tcaagcaatt
     2041 taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat agagaagatt
     2101 tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac ccaaaattta
     2161 atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag agatacttaa
     2221 gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta aaggcaaaga
     2281 tggaaagtga agctttaact aaaaaattag gtagagagcc gagtatagaa gaattagcta
     2341 aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat ttcaatgttg
     2401 agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt attgataaaa
     2461 taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taatatattg ttaaaagaca
     2521 tacttggaag actggataag agagagcgtc aaataatagt tctcagatac tttgaagaca
     2581 tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta tctaggatag
     2641 agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta tgatatgtag
     2701 aataataaaa tataataaat atattactaa aataaaatgt aataactttt tatgttaagt
     2761 ttttaaatta ttaaaagata ttaaaataat aaaaagcatt aaaaaagtaa aaatataaaa
     2821 acaaggtgtc tttatggaaa aatagatttt ttctttatta gacatctttt ttattttttg
     2881 accaaacttt agatagtcaa aataaataat aggtttattt ttttaaaatt aacaaataat
     2941 actttagaga ggtgtttagt atggataaaa attataaaaa atatgtagac caaataagcc
     3001 caaaacctac atatttaaaa aactatacac ttgcatttat tgtaggtgga attatttgta
     3061 tgatagggca agcaataaat gatttgtata tgaaagttgg aggtcttgat aaactaggtg
     3121 ctagttctgc aacatcaata acattaatat ttataggggc atttttaact ggattaggag
     3181 tttacgacct aataggaaaa agagctggag ctggttctat aattccaata acaggttttg
     3241 ctaactcaat agtatctcca gcaatggagt ataaaagaga aggctatgta ttaggtgtag
     3301 gtgcaaactt atttaaaatt gcaggaccag ttttagttta tgggataggt tcttctattt
     3361 tatgtggaat tatttattat atttttaaga tgttttagat gatttttatt taatttaaat
     3421 tttattaatt atgaagaagg tataaaatct gaaaatagct actaaagtaa ctaattgaaa
     3481 aataattctg cagatttaat taatattttt aattaatatt aaaaatttga atagtaaaat
     3541 gcaaaattaa aaaggtgtga aaatatgaaa aataaaagaa ttggaaaaag aacagtcaag
     3601 ttagaaaata aaccaaccat aatatcaact ggaactatag taggtcctaa ggaaggtgaa
     3661 ggaccactaa aggactattt tgatatgatt atgactgacg atttatatgg tgaaaagact
     3721 tgggaattag ctgaaagtaa aatggtagag actgcatctc aacaagctat ccaaaaggca
     3781 ggtaaaaaat tgtcagatgt aaattatatg ctaggtggcg atttaataaa tcaaatagtg
     3841 ccagcatcat ttgcagcaag agaactggct attccatttt taggtatata tggagcttgt
     3901 tctacaatgg cagaaggtct ttgtataggt tctatgctta tagatggtgg ttttgctgat
     3961 ttagtactgt caggtacatc aagtcactat tgtacagcag aaagacaatt tagattccca
     4021 cttgaacttg gaaatcaaaa accaatgaca gcccagtgga cagttacagg agcaggaagt
     4081 gtattattag ctcctaatgg agacggtcca aaagtaaagt atgttactgt tggtaaagta
     4141 atagatgagg gtattgatga tggtaataat atgggaccag ctatggctcc agcagcaata
     4201 gatactatct attcttactt tgaagatact aatgatgatc caaatagctt tgatataatt
     4261 gctactggag atttaggtac tcttggaaaa cagatagcag aagacttttt aaaagaaaag
     4321 ggtgtagata tatcaaaagt ttacactgac tgtggtatag agatgtttaa ccttaaagaa
     4381 caagatgtcc attgtggtgg aagtggatgt ggatgttcag ctacagtatt tgcaggatat
     4441 atatatgata agttgagaaa aaaagaattt aacaaagtaa tgcttgtatc aacaggagca
     4501 ctactttcac caacaagtac actacaaaag cagacaatac catgtgttgc acatgcagta
     4561 gtaatagtaa atgagtagga agtgatataa tatgttaatg gactatgtaa gagtatttat
     4621 tgtaggtgga attatatgtt taatagccca agtaatgatg gattatttca aaatgcaaac
     4681 tccatatgtt atggtaacgt atgtaacaac tggtgtagtt ttaacttttc taggacttta
     4741 tgagccatta gttaagtttg cagcagcagg tgcaacagtt cctataatag gatttggata
     4801 ttcacttgct acaggagtaa taaaatcaat tcaaagtgat ggttttttag gtattttttt
     4861 aggaggtaca actgctacag ctggtggaat agcagcagct atattctttg gatatattat
     4921 gtcagtagta tttactccaa aagcaaaacc ataaatcttt attaaaagaa taataagttt
     4981 ttgaaataat aaatttttat aaagcaggtg gatatctttt agattccacc tattttttat
     5041 ataaagaagc gtttacataa ttgaagttga caattattta caacatagtg tagtattata
     5101 ttatcaaaat aaaatgaagt gaagtagggg ataaagatgg taactattaa agatattgca
     5161 aggaatctag gtatcagtta ttcaactgtt tctaggtgct taaacaataa tcctaatgta
     5221 tctgaaaaaa caaaaaagaa agtggtagaa gaggcaaata gactagggtt ccattttaat
     5281 gtaaatgcaa gaaatttagc aaaaaaagaa actaacagga taggggtaat cttttcaaat
     5341 aattttaatc atcaagatac tagaaagttt tttagtgata taatggatag ttctataaat
     5401 tctattgaga ctaataaata cgattttata atacagccta ataataacat tagtggtgat
     5461 agtaatgtat ataaaatggt aaatggacaa atggttgacg gattagtaat tgtgtctcaa
     5521 tcaataaaaa aagaagagta tgatttttta aaagacaata attttccaca tgtatttata
     5581 tattttaaac catcttttat aggagaagta gacaatttct tttgggacga taacgtgtat
     5641 ggaggttata tagctaccaa gcatttaatt gaccatggtc ataaagatat aataacagta
     5701 acttcagatg ataagagttc aaaaatgcat gaagatagaa ccaagggata tttgaatgct
     5761 atggaagaag caaatttaaa gacagaagtc attaaatcta aaatggattt tgaatctcaa
     5821 gttgaatttt tagaaaaata cattgataaa ataaaaaaag cttcagctat atttgtacaa
     5881 caagatgtac cagcgatttc tataatacaa gagttaaaaa ctacttatgg tataaatgta
     5941 ccagaagata tttccataat agggtataat aatattgaat taatatcata ttttaggtcg
     6001 catttatcaa caatagatga cccaagagag caagttataa aaaatggagt agatagttta
     6061 gtaaatataa taaataaaaa gagtacagaa cacattccaa gaaaattata tccaaggtta
     6121 ataatacgaa atagcgttaa aagaataaag tagtaagctt ttaagtatca aacgtttatt
     6181 aaaaatatta tagattatgt gaagtttttt aacaaataat ataaataaaa attaaaagga
     6241 gtag
//
LOCUS       DAEMHZ010000176         6214 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000176 DAEMHZ010000000
VERSION     DAEMHZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.177"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            374..1186
                     /gene="modA"
                     /locus_tag="KPK72_003475"
     CDS             374..1186
                     /gene="modA"
                     /locus_tag="KPK72_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2490649.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1196..1987
                     /locus_tag="KPK72_003476"
     CDS             1196..1987
                     /locus_tag="KPK72_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490650.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1989..2684
                     /locus_tag="KPK72_003477"
     CDS             1989..2684
                     /locus_tag="KPK72_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2490651.1"
                     /translation="MLKIKNLYLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2671..3402
                     /locus_tag="KPK72_003478"
     CDS             2671..3402
                     /locus_tag="KPK72_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490652.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3716..4516
                     /locus_tag="KPK72_003479"
     CDS             3716..4516
                     /locus_tag="KPK72_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2490653.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFAYKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            5056..5772
                     /locus_tag="KPK72_003480"
     CDS             5056..5772
                     /locus_tag="KPK72_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490654.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2437 a          746 c          856 g         2175 t
ORIGIN      
        1 ctttcttttg tattcaattc tgatataaca cttttagtaa tttgttaata aaaaacaatt
       61 aaaacatatc tatatgtttc aatgttctat tttttcactg aaatataata aatataaaat
      121 tagttaaaaa gtaaaaaatc tacaataagt taatttgatt taagttttta aatttaaaat
      181 actttaaagt attaataaaa attaaaaata aaataactac ctatggaaat gacattataa
      241 atagattttt tcttaactat acataacaaa acataacaaa acattataaa acataacaaa
      301 aacttagaca tcgttttctt agtatgatat actaaaaaag aatttacact aatttactaa
      361 gggggaatat cttatgttta aaaaactttc agtactagct ttagctatga ttatttcatt
      421 aagtattact gcatgtaatt ctaaagaaaa caaagctaat gatgaatcta atactaaagt
      481 gacaacagct gatgattcaa aagaaaaaga gactcttttt gtctattcag gggcaggttt
      541 aaagaaacca atggatgaaa tagcaaaaaa atttgagaaa gaaaataatg tcaaagttga
      601 atatagctat gcaggatctg cacagctaat agctcaaatt gagacaagtc acaaaggaga
      661 cgtatttatt gttggttctg aaccaattta ttcaaaggcc tttgaaaaag aacttgtagg
      721 tgaacataaa acggttgcac atcatacacc tgcaatagtt gtacctaaag gaaatccagc
      781 taatattcaa aaattggaag acttaaataa agatggagtt aaacttattt tgggagacaa
      841 agagtctaat gctataggta agactacaca aaaaatactt gaaaaaaata atttaaaatc
      901 tataaatgat aatgttgtct ctacagctgc aactgtaaat gaaatgattg tacagttaac
      961 atcaagtaaa gctgatgcta caatagccac aaaagattcg gtctttggca atgaagatgt
     1021 agaagttata gaaattgccc aagataaaaa tatagaccaa ctcatatcag caggaatcgt
     1081 aaactatagt gataaaaaag atttagctaa taaatttata aattatatag agtcagacga
     1141 agctaaacag atatttaaaa aatatggttt tgaacctgtt aagtagtgat taaacttgaa
     1201 aaataaattt tttactactc tacttactgg attttttgct atagtctttt tgtttattgt
     1261 atcaccactt cttatgttaa tcattaaagg gataagttat gttcctatat gcttaaaatc
     1321 agtggaagta cagtatgcaa taacacttag tattaaaaca tctttaatat ctactatagt
     1381 atgcctttta ttagctattc cagtagcata ttttttacat attactaaat taccatttaa
     1441 aaaactgatt atacagataa taaatctccc tatgtctctt ccacacttag tatcaggaat
     1501 tgctctttta cttttgtttg gaagaatggg gattggagat tccatttata agatttttaa
     1561 gcttgatttt atatttacaa agcaaggtat agttttagca caagtatttg taaatcttcc
     1621 acttactatt aaaatacttc acacttcact aaatgaaagt aatgaaaaga tgatatttgt
     1681 tgcacgtacc cttggatgta atagttggga agcatttaga tttataattc ttccaaatct
     1741 aaaaactgga atcatatctg caactgtaat gacttggtca agagcacttg gtgaatttgg
     1801 tgcagtagct atgatagcag gttctacacg tatgaaaact gaaattattc ctacatctat
     1861 atatttaaat atgtctactg gtgatataga cattgcaatc ggaatagctg taattttgat
     1921 atttatctca cttacatgct taatgctttt tgagatattt tttaatagag aggttgataa
     1981 gaattaatat gttaaaaata aaaaatttat atcttgattt acctaatttt aaattacaag
     2041 aaaatagttt aaatattaat aatcatgaat actttgtttt attaggagct agtgggtcag
     2101 gtaaaactct atttctagaa acattagcag gaagatatcc atctgctaaa ggaaatgtat
     2161 catacaaaga aaatttaata tcaaacttac ctccagaaaa tagaaatata ggatttgtat
     2221 atcaaaattt tgaattattt ccaaatatga atgttgaaga taatataagg tttccactta
     2281 aattgagaaa aatacctata gatgaacaga ttcttaaggc taaccacttg tctaaattaa
     2341 tgcagataga agatataaaa aataggtatc ctaaaaattt aagtggaggc gaaaagcaac
     2401 gagttgcttt tgcacgtgcc ttaatagctg aacctgatat attactttta gatgaaccta
     2461 tgagttcatt ggattatatt acaaaaaaac atataagcaa aattttgaag gatgtctaca
     2521 taaaatacaa accaacagtg attcatgtta ctcatgatat ttctgaagca atgttttttg
     2581 ctcataagat aggaataatg aacaatggaa aaataaatca tatttttgat ttaaatgaag
     2641 aaataaaaaa taaaggagaa tcttttttct atgaatatat ttgatatttt gagagattat
     2701 ctattagtac aagtcgataa atataattta aatatggata tgataagtat tgtgtccaaa
     2761 tctctatctt caaaagaagc aattggaaat actaagcgta aagatttccc aattatagtt
     2821 ggtaaagaaa taatgcttga agctgacttt aaaggagcta aaggacaagc atttacttct
     2881 actccatcta catttgaagg ttctttaaaa gacatattat cattagattt acatgacaat
     2941 tctcatgata gaagtctttt tatagcatca ttaaatgcag ttatgaaata cttaggcaag
     3001 acggatagaa ccattcattg taaaaataac gagccagagg tatgtgcaaa aaaattccct
     3061 gagtttataa aaatggaatt tggtaatcca aaagtagcaa ttataggata tcaaccagcc
     3121 ataattgata atattaagga ctttttcgaa acaagagtat tagatttaaa tcctgaattt
     3181 gtggatacta ttcaatataa tgttaagatt gaagatggta tacgagatta tgaagatgta
     3241 atatcttggg ctgatttagt gatttgcaca ggaagtacat tatgtaataa ctcaattata
     3301 aattttctat cacttaacaa accagtttac tattatggaa ctacgatagc tggtgcatca
     3361 aatattttag gtttaaaaag actctgtttt tgtagtaaat aagcaatcat atcatcaagg
     3421 agtacacaaa tatatatgtg tactctttaa atttaaaaag ttattacttt tattctaagt
     3481 actaaagtaa aattctatta aaatgaatcc cgaataaatg tacaatccct ttaaatctca
     3541 attcctatca atcattaata ttttgattag tcttatacat aaatgttttc tttattttaa
     3601 actaataaaa aaattttatt ccattaatct actctatatt tttcagcatt taagaaataa
     3661 tttttagtac tttgtttgat aatataacta agacaacctt tcaggagatg attaaatgac
     3721 atttaagaat aaaaaattta ctataaataa atataaaatt ctatctatta ttctagtttt
     3781 atgtttgatt ttaaccgctt ttagtagcta tgtgaattat agtcataaaa aatttaaaga
     3841 ttatgttgtc ttctatgttc agcatcaaga tgatgaagtg ttgtgggctg ggagtgctat
     3901 tgttaatgct ataaaagaaa gaggaaaaaa tcatgtattt gtggttttag tatctactgg
     3961 atgtggtata tccgtctttg ataaatataa aaaatatgaa aacctaacaa atatacaaaa
     4021 atcagaatat agaaatagag aatttttagc ttctcttaat agccttggtg taataaggga
     4081 aaatataata ttattaccag aaacaaatac aaatggtaat actgacttca attacatgga
     4141 aaaaattgct cttacatttg aagaaaaatt taaaaatgta actcatatag cacatactta
     4201 taagttagat gaccatttac aacatttaaa aaatggttct gttatccaaa atttatataa
     4261 tgctggtaaa ataaaagatg ctaaatattt tgtaaaacct aaatttgctt ataaaataac
     4321 ttttaataat aagatagttt ataaagctca ttctactgaa gactatgaaa aggttaaaaa
     4381 tgcctgtcaa caatacaaaa ttgttgatga attagaatat agagaaggta ttggctataa
     4441 gtctgaccat aaatcatttg ataagttatt aaataataaa gatgttcctg ctatacttca
     4501 tacacctaat ttataagaaa tatttattct tttaatttaa gttataaaaa ggaaattatt
     4561 tgagattttg tgatatagca ctttttgtgc tatactactt tttctcatac aactattacc
     4621 catatctaat ttcatcatct cttaaaatat ataaagatat catcattaaa acctataagc
     4681 cttattaaat gctttataca tacctacatt aaactacctg tttatattaa actacattaa
     4741 aataaatatt tgttataaat tcttaataat tatgaaactc atttgttatt tatatttgtt
     4801 aatatatgtt taaacctaaa aaattaataa accaaaataa cataagaagt gtgatagatt
     4861 accctagata ttttcataaa cttttacctt cagattaata tgggcgtcta tacttttaaa
     4921 tttactatat gcattgacaa tatattaaaa gtactagttt ttatttcata atgaacgggc
     4981 ttactgtttt ctaatacctc tttttggtat aaaataaaaa ttggttaaaa ataaaatttc
     5041 aagggggtta ataaattgaa tcaaaatata ttggttgtag atgatgatag agagatagtt
     5101 gatgctatag agttctattt gagacctgat aactttaatg ttttaaaggc atatgatgga
     5161 cttgaagcat tggacatact aatcgaaaat gatattaaat taataataat ggatgtaatg
     5221 atgcccaatt tagatgggct taaaactaca cttaaaataa gagaaaaaag gaatatacca
     5281 ataattctct tatctgcaaa atcagaagat atggacaaaa ttataggtct taatatgggt
     5341 gctgatgact atataactaa accatttaat ccactagaac ttactgctcg agtaaaatct
     5401 caacttagaa ggtatataaa tttaggtgat tttagtaata caaataactc aaatgatact
     5461 aattatattc taaaaagtgg tggcctagag ctaaatacag atacaaaaat agtttcttta
     5521 gatggtgagg aaattaaaat gacaccactt gaatataaaa tacttacact tcttatgtca
     5581 cgaaaaggta aaattttctc ttctcatgag atttatgaaa atgtctggaa tgaggatgct
     5641 tatagttgtg agagaactat tgctgttcat attagaagaa ttagagaaaa gattgaaatt
     5701 aatccaaaag aaccaaaata tttaaaggtg gtgtggggca ttggttacaa aattgaaaaa
     5761 cttaattatt aatgattcta aagcaaaatt tattttatta ttattactgt ttctattagt
     5821 ttctatatta tttgaagcag tttatgattt gaataataac ttttctaata ttaattatta
     5881 ttatagagag tttgctctcc cttattttta ttttttcggt actcctgtac atttgtcaaa
     5941 agtagaatta gagtttgtct gcataggtat tatattatca agttttttat tgactatact
     6001 atgttttaca ttgtataaaa aaaataaaca aactaaagtc aaattcttag agaatgtcat
     6061 aagccttttt tctaaggtaa aaatagaact tacacttata ttaactttga tactattaac
     6121 attaaggttt catgcattta caagtaataa aatattttac aacactaatt taataatcta
     6181 caattttata attatatcta tagtctatat aata
//
LOCUS       DAEMHZ010000177         6148 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000177 DAEMHZ010000000
VERSION     DAEMHZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.178"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            186..1067
                     /locus_tag="KPK72_003481"
     CDS             186..1067
                     /locus_tag="KPK72_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG2490655.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1449..2252
                     /locus_tag="KPK72_003482"
     CDS             1449..2252
                     /locus_tag="KPK72_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490656.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2264..3394
                     /locus_tag="KPK72_003483"
     CDS             2264..3394
                     /locus_tag="KPK72_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2490657.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISRSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            3398..4807
                     /locus_tag="KPK72_003484"
     CDS             3398..4807
                     /locus_tag="KPK72_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490658.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPVYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            5018..5527
                     /locus_tag="KPK72_003485"
     CDS             5018..5527
                     /locus_tag="KPK72_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2490659.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2497 a          540 c         1089 g         2022 t
ORIGIN      
        1 aaaattatat agttaatata attttataat tggtttagta tagagatttt taacttttat
       61 gttatactat ttataaaagt aggtataaag attaaaatac ataatcaaat atgtataaga
      121 ggttttaaat agtattgtta atgaacttga ttattgttta taattacaaa ttggaggaga
      181 taaacatgaa aattactgaa ggatatatgc cttttaaagg ttttaaaacg tattaccgta
      241 tagtaggaga aaatacggaa gggaagaagc cattagtatt acttcatgga ggtccaggtt
      301 ctacacacaa ctattttgag gtattggata agatagctga aagtggtaga caagtaataa
      361 tgtatgacca aattggttgt ggaaattcat ttgtagaagg gcatcctgag ctatttaatg
      421 cagatacttg gatagaggag cttattgaac taagaaaaca tctaggactt gatgaaattc
      481 atctgttagg tcaatcttgg ggaggtatgc aggctatttg gtatgctatt gaatataaac
      541 caaaaggtat taagtcttac atcctttcat ctactctttc ttctgcaaaa ctttgggaga
      601 aagaacaaaa aagaagaata tcatatatga gtgaggttga ccaaaaagca ttacttgatg
      661 cagtaaatac tggagattac tctagcaaag aatataatga tgcattagaa agatttatgg
      721 aaatgtattg tgcaggtgaa gtgacagaag attctccaga gtgcctgaga agacctaaaa
      781 aatcaggttc tgaagcatat attgtaggat ggggacaaaa tgaattttct cctacaggaa
      841 ctctttctgg atatgaattt acagatagat tacatgaaat aaaagagcct tgtctagtaa
      901 ctagtggagc aatagattta tgttcaccat atattgcaaa gactatgtat gatagaatac
      961 ctaatagtaa gtgggaatta tttgagtatt caagacatat gccattcgtt gaagaaaatg
     1021 agaaatatat aaaagtctta actgagtggt tgaatgcgaa cgattaaaaa ctatataaac
     1081 ttatataaaa aaatgagtgt ctcaaaatga actttttagt tcattaggca ctctttttgc
     1141 atgaaatcaa atatattttc attatttgaa cgataaaatg aggcttatat ctattttggg
     1201 aaagtaattt tttattacta acgttagatt ctaatctaat tgtatactta ttgaaataat
     1261 agaaatatat tggtgtttat aacttaattt tattgttttt tagaaatttt tttatatatt
     1321 ggtataatta ttctgtttaa atttgattaa aaagtatatt aattataata agaaacaatt
     1381 atataaatag gaatagatta ttataattaa tatagatata aaaataatta tagaaggaaa
     1441 ggcacattat ggaaaaattg agagttattg aaatgataaa aaataatccc aatattatag
     1501 ctacaataga taatccaact gatgaaatga aattgttagc tattaaagaa aatgggcttg
     1561 tactagaata tataaataat ccaaccagag agatgcaaga actagcaata gataataata
     1621 ttagagcaat taaatttata gataatccaa cagaagatat gatgataaaa gcagtaaatg
     1681 agggatggag tatactggat tatatcaaaa acccaactga taaagttatt gaaatggcta
     1741 taaatcaagc aggatgggca attaaatatg ctaaaaatcc aagtgaagaa ttacaactgt
     1801 tagcagtaag aaaaaattat gattcgataa agtttataaa acaaccttat gaaagtgtac
     1861 aagaggaggc agtgagaata agttatgatg ccttgagata tatagattca cctagttata
     1921 atgcagaact tatagctata aaaaataatg aagcagctat tagctttata actgatttag
     1981 ataaaaataa aatgttggaa tttttaaaag tgaatatttt agtgattaag tacattctga
     2041 aaaaagtatc taaagatgaa ttagagagtg tattaaaaga agtattatcc aaagaagatg
     2101 ttgaggaaaa gtatattaga gattttttga attgtagtgt tatagataga aatagtgaaa
     2161 gtataccaat agataaaatt atgtttattt ataaatatgg aagtaagaca gcaaaaaaaa
     2221 tagctgtaga tgagaaatta aagattatgt aggagagaga aaaatgaatt ttatagacac
     2281 attaaaaagc gttgacttaa tattatccac tggagaagta cctttaatag taggtgaaag
     2341 tggaatagga aaaacagctt tagcaaataa gcttgccaaa gaaaatgatt ggagtttaat
     2401 tgttattgat ggaaacttac ttaaagaggg tgaaataggt ggtcttccaa ctatagaatc
     2461 ttatgtagga gttaattcta atggatataa gactgaaaag aaaacaacag tgtatgctgt
     2521 tcataacaaa ttaagagaaa ttgatgaaga gatatctaga agtaagacag ttcttttatt
     2581 tatagatgag ataaatcgtt gtgagcatac agtacagcaa gaacttatga atttaatttt
     2641 aaatagagaa attaatggat ataaactgca tgatgatgta aagattttag cagcaatgaa
     2701 tccatcaagt aaatatggtt cagattttga ttatcaagtg gtagatatgg atgcagccca
     2761 agaaaatagg tttgtttggt taaatatgga gagtgaccat acacaatgga taaaatgggc
     2821 aatagatgag ggtattgaaa gaaaagttat cgagtttatt tccacttttc cagaatatct
     2881 gcataaaata aatgaagatg atgtaagagc tactcctaga agttatgaga gagtatctaa
     2941 aatttataaa gtttataaag aaaaaaataa ttctattcct agagcagtat ttttaaatgt
     3001 tgtaaagggt aatgtaggta aagttattgc agaagaattt ataagtttta ttgaatcaaa
     3061 ctctgaacca ttaatatcct atgaagatgt ttttttagga gaatctattg atgagtctat
     3121 tgtagaaaga gtaaaaaatg agagtcatac aagactttat ctatcagcta tgaacattct
     3181 aaaagactta gaattaaata ttaagaatga taaatatgaa tcaaaccact atattaatag
     3241 atttattgaa tttttaaaaa tgtatcctgt agatttaatg ataggtatta tgaaggatat
     3301 tagaaacagt tatattgaag tatataaaaa agctatagaa aatgaagagt ttgtaaaatc
     3361 atattttgaa tcttatagtt taataagggg ataatgtatg gaaaattatt ttgataaaca
     3421 agcaaaagag ctttataata aagcaagtga aattataaat actcattcta tgctaaaggc
     3481 aaatcgtgct aatgagaaat ttgatataga tataccacaa gattttaagt atgagttttt
     3541 tagtcttgta gataaagtga atttaagtct tatggaagaa gaagataatt tttatggata
     3601 ttttttgttt cagatgtcaa gagaaataag atttgatata agtagtccta caggtgtgaa
     3661 ttttaaggga gctaaatatg ttatatattt taatccaata atctttttaa cgcttgacat
     3721 aaagcaaatg gagactacaa ttaaacatga aattcatcat atattatcta tgcatttgat
     3781 gagagcaaag gagttaaaag gaaagtatag tacattagca attaatatgg caatggatat
     3841 agtagtaaat aaatttctaa acaatttacc accatatgca actaccttag agtgggtaaa
     3901 tttaaagtat tccttaaaac ttgaaccata tgaacctttt gaatattatg tagaaaagat
     3961 tcagactgaa ttagatttga tggaagtgga tgaagatggt gaggaagatg atagtgataa
     4021 atatggagat atagaaattg attatagccc cgaaagaact catgatatat gggatgaatc
     4081 caatgagata gatgaaaata cccttagaga atttactgaa aaatttatta acagttctca
     4141 aaaaggtaaa gttcctgttt atttagaaag tatgatatca tcacttaaaa atagtaaggg
     4201 tgaattgcca tggaatttat atcttaatag aataatggga acagttgaaa ataataaaaa
     4261 gaagactata acaagaagaa atagaagaca accaaatcgt ttagatttga ggggagaact
     4321 tagaagtcat aagtcagaaa tagcagttgc acttgatgta agtggaagta ttagtgatga
     4381 agagtttaaa caagctatta aagaagttct tagtatagtg aaaaactata atcatgaaat
     4441 tacaattata gaatgtgata atgaaattag acgtgtatat aaagtgagaa ctgaaaaaga
     4501 tataaaagat agaattagga taggtggagg tactagattt tctccagtct ttgagtatgc
     4561 taataataaa aagattaatt tgttagtcta ctttactgat ggtaaaggtg aaaataaact
     4621 acaagtaata cctagagggt ataaaatttt atgggttatt tctggaagag gagataaaat
     4681 ttcattaaaa gaaccttatg gagcagttaa aaaacttact aaggttaaaa caaatgatga
     4741 aatattagaa ataaatgatg taagaaatga tgggtactca atgaatagcc aagcaccaat
     4801 tttataggta ttcacaggag ataagctgta ttaatttatt ttgatatgct tgttataaaa
     4861 atctaaaaaa tacattaatc aatggttaat gtatttttta attttaataa tataagtttt
     4921 acttacattt aagtcttaaa atgtgttaaa atataatata tagcagatag ttttggcaag
     4981 gtggatttag ttgtcaaaac tatttttttt attaaggatg gtgatgtttt tgtttaagat
     5041 tgatgattat ataatgtatg gtatgacagg agtctgcaaa gtcatggata taacgaatga
     5101 acgatttaca aatggaataa aaaaagaata ttatgtattg agtcctattt attctaataa
     5161 tacagtaata aaaattcctg tagataatga aaaagtacca atgagaaagt tactttctaa
     5221 aattaatgtg ctttcattaa taaatgatat tcctaatatg gatacatcat ggatagataa
     5281 tgaaaaattg agaagtgagc aatttaaaaa aatactcaga ggtggtaaat gtgaagagtt
     5341 aataaaatta gtaagaagta tagataataa cagagagtat gtaaaatcta ttggtaaaaa
     5401 aacacatcaa gcagatgata atataatgaa agaagctgaa aggttattaa atgaagaatt
     5461 tgcaactatt ttagatattt ctcctaatga agttagttca tatatttcaa gtcatatacc
     5521 tcaataaagt gttttagtat cctttattta atctatttta agtattattt atgtatgctg
     5581 gttttggtaa aataaaatat ataattaaat tatataaaaa ataataatga ttgtattgaa
     5641 gaattacaga atagtgcgga gagcctactc ctttaggtgt gagatggata tcacttaaag
     5701 atttaaattg ttattgtaga tgtttttaat ataaaacaag attaataagt gtattgtaag
     5761 ctttaagtga aattgatact ataatgagtt acttagattt taaaatatat aatattataa
     5821 aaaatccttt taggttttga atgaataaga tggatactat gttgaataga atccatctta
     5881 tttaagtttt atttatgttg tttatttgta agcattattt ttgtacatga aaacacagtg
     5941 taaaaatatg cttagaatta tgaagtttta ttaaaaagat attcgacaaa ataagactaa
     6001 aaatattctt aaacttataa ctaagataaa tattgtatat ttatagtgta gcctgaaaaa
     6061 ttgtggatgt acgaaattta tttagtattt tattgaaaca aagtagaaga agtactatag
     6121 tttatctttg aatagattgt tatttttt
//
LOCUS       DAEMHZ010000178         6125 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000178 DAEMHZ010000000
VERSION     DAEMHZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.179"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            459..716
                     /locus_tag="KPK72_003486"
     CDS             459..716
                     /locus_tag="KPK72_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490660.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(826..1506)
                     /locus_tag="KPK72_003487"
     CDS             complement(826..1506)
                     /locus_tag="KPK72_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2490661.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            1766..2065
                     /locus_tag="KPK72_003488"
     CDS             1766..2065
                     /locus_tag="KPK72_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490662.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            2389..4044
                     /gene="ilvD"
                     /locus_tag="KPK72_003489"
     CDS             2389..4044
                     /gene="ilvD"
                     /locus_tag="KPK72_003489"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG2490663.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELETLAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGIVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            4388..5020
                     /locus_tag="KPK72_003490"
     CDS             4388..5020
                     /locus_tag="KPK72_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2490664.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            5043..5855
                     /locus_tag="KPK72_003491"
     CDS             5043..5855
                     /locus_tag="KPK72_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2490665.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2440 a          677 c          991 g         2017 t
ORIGIN      
        1 taaaatatat tgaaaattac catttgtata tgtataatat gaattataca aatggtaatt
       61 tgatgagagg tggtatgttg aaataagaaa ctgaattaat taataaatat atagaaagtt
      121 ttgaaaaccc ttaattgaga tataaacaat atgtacaaaa ttttttgcaa gtaaaattac
      181 caaatgtata aaataaatca gtagcataga taattttatg ctttaataca ttagattatc
      241 taatttagtt ataatggtca aaaatgcaat ctacaaataa aacaaagtat tatcaaaaaa
      301 gtattcttta agagaatttt ttattagaga tgtaattgat ataaataatc gtattaaaaa
      361 ataaaatact aagtttaaaa atttaaataa tctataaatc aattataaaa tattatttct
      421 tagaaattaa aatgaattaa gtaaatggag gaattgatat gataataatt agaagtcaag
      481 ataaaactaa tttaatgcag ataaaccaaa ttaaaataga tggtagtcaa gtttatgcag
      541 tatttgaaag taaaattgat actgtaaaaa taggagatta tgaaaataat acaagagcta
      601 tacaagtgtt ggacaatata caaaacttca ttgaaaatgg aacaaaatat gattatataa
      661 catcaaataa agtcagatac aatgttaata aaatatttca gatgccagct aaatagtgag
      721 tgtgcatttt agtttaataa ctaaagccca ctttcttttg tagtatgaaa gagggcttta
      781 catatttgta aatatcgaag tacacttgtt ttatcaataa attatttaat aaggtataat
      841 ggaccaatta aacaataaaa attctcctaa aacagcaagt attaaatata taatagcttc
      901 atttttgtaa agagaggtaa ttttttcgct tatgtcatca tcaattgtaa tagcaatttc
      961 atcttgaata atatttttat atatagatgg gaaaatatct tttttcttta taagattaaa
     1021 taaattattt acacctaccc atatcaaata tcctaaaaca gcacctaatg tgttcatcat
     1081 taaatcatca atatctgtag ctcttaagtt aaacaattga caaagttcaa ttgctaatga
     1141 aaaacctaat ccagttaatg aaacattgat aatatttcta aaatttttcc aaattaaagg
     1201 tagtaaaaat cctaaaggca taaacataat tatatttaaa atatatgtta agatgccaac
     1261 tgagtcaaaa ggtattagat ttatttcatc aattctaaaa agcccactat accttcctat
     1321 atcccatatg ctaccaattc cagctttact aagtgctaga tagatatata ataaaaaaat
     1381 atatacccat actaaatgta tcattacatt cttttcaact ttcaaatttt ttcgttttat
     1441 aacgactatt tggtatatta agcatggtaa tataacgcaa ataaatgaat aaaatgtcca
     1501 tatcatttta ttttgtcccc tttcaaattt atgtttttag ttttataagt actatcttta
     1561 tattaaactg taagttttaa aaatctttta ataaaatctt aaaaatttat taatttataa
     1621 aaatatatta ttaagattaa aaaatatata taatcaaagt ttctacaatt atatagttag
     1681 ggatataaaa attttatcgt atgtataaat attataaata tatggaaata ataatagtat
     1741 attttggtaa agtaggagga attttatgaa atggattaaa tggtatagta ttacatgcat
     1801 atgtattttt atagtagtag ctttttatat gtttatattt cctaataaaa tagaaactat
     1861 tgatacttca tctgcatata gctttgttga aaagaaagta ccaaatagtg ctgtgtatca
     1921 aggatacaaa aagaatccag ttgatggtac tactacaatt tattacagct atgataattc
     1981 aactcatata gttagattat cacatccaga agactattca aggaagatta attgggataa
     2041 agtttcaaac atcagatttg attaatcttt aatatttata aaagtctata tatctaaggc
     2101 aaatatatta ttaatcttat tcatagttga gcactttcta ttatagaagt gcctcttctg
     2161 atttatatca taaaatatta ctcaacaatt tgtaaaatat attagtttat aaaatatatt
     2221 gaaaagataa tattatataa tacaattgtg taagatattg ataaaaaatc gtaataaatt
     2281 ttcattagta tatgctaata tttttttaca attttcgtaa ttaattaata gaaaaatata
     2341 ttataataat attatgaaaa tttaaaaaag taagaaagag gtaatattat gagaagtgat
     2401 ataaaaaaag gaatagaagg agctccaaag agagcgttaa tgtatggaat gggtcttact
     2461 aaagaagaaa ttgaaagacc attaataggt atagtaaatg cacaaaatga ggtgattcct
     2521 ggtcatctgc atcttgatga aatagcagaa gctgcaaaaa atggagtaag aatgagtggt
     2581 ggtttgccat tagaatttcc tgctataggg gtatgtgatg gtatagctat ggggcatgtt
     2641 ggaatgaatt attccctggc ttcaagagaa ttaatagcag attcaataga agctatggct
     2701 atggcacatg gttttgatgc tttggtactt atacctaatt gtgacaagat agtgccaggt
     2761 atgttaatgg ctgctgcaag acttaatata ccaagtattg ttgtgagtgg agggcctatg
     2821 cttccaggta aaaaaaatgg aaaagtatat gattttaact ctgcaatgga gggtgttgga
     2881 gcatgtaaag atggaacagt aagtgaagaa gaattagaaa cgttagccat gaactcttgt
     2941 cctggatgtg gttcatgttc tggtcttttc acagcaaata gcatgaattg tttaacagaa
     3001 gctttaggca tgggtatacc atataatggt actgctgcgt cacattctgg agaaagaaaa
     3061 aggatggcaa aatatgcagg tatgtatgtt atggagttac ttaagaacga cataaaacct
     3121 agagatattt taacaataga tgcttttaaa aatgctatag ctgttgatat ggcaatggct
     3181 ggttctacaa atacagtact tcacttacct gcaatagctt atgaatcagg aatagagctt
     3241 aacttagatt tttttgatga aataagtgaa aaaactcctt gtttaacaaa attaagtcca
     3301 agtggaaaac atcatattga agatttacat atggcaggag gaataccagc tataatgaac
     3361 gaactttcaa agataaatgg aataaattta gattgcaaaa ccgtaacagg caagactata
     3421 ggggaaaata taggaaattg tgaaatagaa aatgaagaag taatacatac attaaagaat
     3481 ccatatagta accaaggtgg gcttgcaata ttgaaaggaa atcttgctct aaatggagct
     3541 gttgtaaaaa agtcagcagt tgcagaagaa atgttagttc atgaaggacc agcaagagtt
     3601 tttaattcag aagaagaagc tgtaaatgct atttttggta aaaaaataaa caaaggtgat
     3661 gttatagtta taagatatga aggtccaaag ggtggtccag gaatgaaaga aatgctatct
     3721 cctacatcag cagttgcagg aatgggactt gataagcatg tagcacttct tactgatggt
     3781 cgtttttcag gggcaactag aggagcatcc ataggccata tttctccaga agctatggaa
     3841 ggtggtttaa tcggaatagt tgaagaagga gatataattt ctataaacat accagacaaa
     3901 aaattagagt taaaagtaga tgaagttgaa atagaaaata gaaaattaaa atttaaacct
     3961 ttagaaccga aaataaagca tggatactta agtagatatg ctaaattggt aacatcagca
     4021 aatacaggag cagttttaaa atagtagact atatttatgt aaattagaaa aagttaagaa
     4081 aatattaatt ggatatctta ggtgttatag cactaatgag gtatcctttt tattttgtat
     4141 gaaaaacaca gcaaaatctt gattatcttt gaaaacaata catctctatt ataaggaata
     4201 cgagtacagc atagagatgt atcttttaga ccttgttgag gtaatacaac tttttaaaag
     4261 tttgatgtgg aatgcttttc gttccatctg aactatgaag aaattgaaag tcagttaatt
     4321 cttgacataa taaatattaa aatgtataat aaaaaacaaa cagacagtct gtttttagga
     4381 gggaaatatg aatagagaag aaaaaagtaa aaatagtaaa gaaaaaatta tccaatcagc
     4441 attttcacta ttttcgtcta aaggatatga ttcaacatct acacaagata ttatcaattt
     4501 atctggtcta tctagaggtg caatgtatca tcactttaaa actaaagaag atatactgag
     4561 aagtgtcaca aaagaacttt actcacaaat gaataatttt ttagagcatc ttgttgctga
     4621 tgacaccctt acagcaaatg aaaaaataat agaattggtt gttcatagtg cgaatgatta
     4681 cacacgtaga aaaatggtac attgtagctg gttagaaaaa atcccattcg ctttaataga
     4741 ggaagttcgt aatctcaaca atgtagttgc acccaatatt gctaagataa ttaaacaagg
     4801 tgttgaaaat aaagaatttt cttgtgaata tccagaggaa ttagctgaaa tgctcgtttt
     4861 tagtattgat attttacttg accctgtatt atttaaacgt gaatacagtg aagtatgtaa
     4921 taggttggat tttttattat tcatgttaaa aaaaatggat attcctctaa ttgatgagta
     4981 tggtattcaa aaatttaaag atttatttag acaattataa taggaaaagc gaggaataga
     5041 ctatgtttta taatgcaaaa agttgtcaaa tgatgatagg aagtaataca gtgaattata
     5101 ttgaatttgg tagtggtaag aagacactaa ttattttacc aggattagga gatggtttat
     5161 ttcctttaca tggaaaaata caggcgattg cttttgcttt tagatataag caatttgcaa
     5221 aagattataa ggtttatgta tttagtagaa aaaatcaaat tacagaaaaa tattccacaa
     5281 gggatatggc taaagaccaa gcagatataa tgaaaaaact tggaattatg aaagcagaag
     5341 taatgggtgt ttctcaaggt ggaatgatag ctcaatatct ggcaattgat tatcctgagt
     5401 tagttgagaa acttgtgttg gcagttactt cttcaaagca aaatgatact atccaaaatg
     5461 ttatttgtag ttggattgat atggcgaaga agcagaatta caatgattta atgattgaca
     5521 cagcaaagaa atcctattct gaaagatatt taaaaaaata ccaattattt attccttttt
     5581 taggaaaagt aggtaaacca aaggatttta aacgatttat tattcaagcg acctcttgca
     5641 tagaacataa tgccttttcc gaattaaata aaataacatg tccaacctta attataggag
     5701 gtgctaatga taaaattgtt ggaaataatg cttcttttca tttagcagaa aagattaaaa
     5761 aaagcgaaat atttatctat gagggattgg gacacgctac atatgaagaa gcacaggatt
     5821 ttaatgaaag agttttagag tttttaaata agtaaacacc tttgttaaaa atatacatat
     5881 gaagattgga aatttaatgt taaaaataga aacatgaaaa tatgcttaac tggtattttt
     5941 actattcata accaattttt gatacattat ctactataaa tacaaatata gcttcaatgt
     6001 gattatatat tagtgtattg gtaaagcact tatacaaaca gaggaatttt gtaaattcat
     6061 attataccca catttgttaa cttatgaaaa tataatcaaa atttttatga gatttttata
     6121 taaaa
//
LOCUS       DAEMHZ010000179         5985 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000179 DAEMHZ010000000
VERSION     DAEMHZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.180"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..516
                     /locus_tag="KPK72_003492"
     CDS             <1..516
                     /locus_tag="KPK72_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2490666.1"
                     /translation="TNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSIDILNYPE
                     YESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGYKNTKVCVHITLNERIEII
                     ISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNISVESIPG
                     LGSSFYITFPI"
     gene            745..3222
                     /locus_tag="KPK72_003493"
     CDS             745..3222
                     /locus_tag="KPK72_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2490667.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSIILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPYAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            3237..3923
                     /locus_tag="KPK72_003494"
     CDS             3237..3923
                     /locus_tag="KPK72_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490668.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            4197..5030
                     /locus_tag="KPK72_003495"
     CDS             4197..5030
                     /locus_tag="KPK72_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490669.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5068..5523
                     /locus_tag="KPK72_003496"
     CDS             5068..5523
                     /locus_tag="KPK72_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2490670.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFIIQFQ"
     gene            5560..5799
                     /locus_tag="KPK72_003497"
                     /pseudo
     CDS             5560..5799
                     /locus_tag="KPK72_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
BASE COUNT         2303 a          642 c          938 g         2102 t
ORIGIN      
        1 acaaatacac ttataaacga tttaaaatta acctatcaat taaaaaatgg aataatccct
       61 ttaaataaaa ataattatga tttagtaaga tttttgaaag aattatccat tgatatttta
      121 aattatccag aatatgaatc aaatatagtt gaatttaaga gtaatataga taatattaac
      181 tttgattttg attgtacact attaaaacga gcttttagta atcttattgt caatgctttt
      241 acatatggat ataaaaatac taaagtttgt gtacatataa cattaaatga aagaattgaa
      301 atcattatat ctgataatgg aaaagggatg tcaagagaag aagcatcaaa tttgtttaat
      361 cgttattata gaggaagcaa tacaaaacaa aaaatagaag gaactggttt aggactagca
      421 ataacgaagc aaattattga agttcatgga gggaatatat ctgttgaaag tatacctgga
      481 ttaggtagct cattttatat cacctttcct atttaattaa ggttaattaa ggttaattaa
      541 ggttaaataa aaaatatatt aaggttgata gattattata tttatatagt agtttatcaa
      601 tcttttttat ttaaggggtg atttgattaa aaatattata aatagaatga taaaagtatg
      661 gaggagtcta ttagagattt aatttagatt aaagtataaa aatattagat tgaaatatct
      721 tagataagga aagaaggtgt aaatttgaaa attatattaa agtatatttt cacaaatatt
      781 aaagaacgaa aaattagaac agcagttatg ttattgtcca tagttttatc aacagtttta
      841 ctttttgttt cattctcaat tggattatct tatgaaagtg cacaaagaaa aatggcaaaa
      901 ggtatgtatg gtacagcgac tatatctgtc caaagtaaaa atccagatat attaaccaat
      961 ttagaagaca tacctgattt aaatactata aaatcaaagg ttggtgtatt ggaaagctct
     1021 gctatttata ataaaggggg atactatgaa gaatttagtc ttatatcagc agacttaagt
     1081 caactaaata aaatcaacaa gccacgatta gaaaatggcg atagtattac tgatttttct
     1141 ggtgataaaa ttattcttcc aaatcgcttt acatcaaaat ataaaataaa aaaaggtgat
     1201 tctattactt tacagattta tggaaagtcc tatacttttc aagttagtga tattgcatct
     1261 tatgatacag tatttttgag gaacacaaga ggtgtaaatg cactgctacc caaagagact
     1321 ctatcaaaaa ttataaataa aggcagtgga tatacaagag ttcttataga gtcagaagaa
     1381 gaaatgacag aaaatctagt aaataaatta tccgaagaac tttctactga aaaatatatg
     1441 gtatcaaata ctataaatga aactaagata atttcagatg ctagacaaaa gacaatgcct
     1501 ttctttttaa taagtttttt tgcattaacc cttagtatat ttataatata cagcagttat
     1561 aaggttatta cattagaacg tcttcctttt attggaacat ttagaagtat tggtgccaat
     1621 gaaaaaactg taactcgtat attgatgctt gaaagtatat tatacggaag tataggagga
     1681 ttaattgcta ttcctattgg tgtagttgtt ttaaatttaa tgcttcatgg gttaggaaat
     1741 tctctcgagc aaggaatatc aattccagta gtaatatcac ccattggagt gattatatct
     1801 gttatagtag caataatcgt ttcttcattt agtgcatata tacctgttaa aaaagctagc
     1861 catttgccga taaaaaatat tgtactagga acagtagaag aaaagaatgt ttcaaatcgt
     1921 tctatacttt ttataggaag tataatgttt attttatcta ttttacttcc aagaatatcc
     1981 cctgaaaata cattgtatct agctggtgga ttttcattac tgggattaat tgtagctact
     2041 attgtgttga ttcctcttat tactgatatc atgtctatag tttttgagtt tgtttataaa
     2101 aatattttag ggaatgaggg taaattagct gctagaaata tgaaaaataa caaaaatata
     2161 atacagaata tcactttact atttataagt atttctgcag ttattgcaat aagtgttgtt
     2221 ggtaactttg tcaaaactta tataacagat gtgtttcgtg acgcagaatt acaaggtttt
     2281 gcagatggaa agatgaatga agagtttatt gaagatgtta gacatatgga tggaattaaa
     2341 aaaatattac ctctttatgt tatgaataat gagatttctg gtaatggtgt aacattatca
     2401 agactagagg gtacagataa tataaaacta tataactcta tgtttggaat caattataca
     2461 aattttgaga taaaaaaaca agtaatagaa gcttttaatg ataaacgttc tattattttg
     2521 aatgaagata ctctaaaaaa agtaggctta tctataggag atacaataac tctatctaat
     2581 gataagtatg atttttctta taaaatagta ggtagtttta aatcccgtgc aaatgatgta
     2641 gaagctgtta taccatcaca ttatgcagtt aatgattttg ataaaactaa ttatggtttt
     2701 ttagtatata cagcagtcaa tccatatgct gttatgattc agataagata tttatttgga
     2761 gatacatata attggagtcg tacagttgaa gaatttaata atgattcttt aaatactata
     2821 agtagttttt tgtctcctat gaataagatg acctatttca tttttcttct tgctacagta
     2881 ggtattatta ataatctact gattaattat attcaaaaaa gacgttctat agctatgtat
     2941 aaatctatag gactaagtga taaacaaaat ataaaagtaa cattgattga agggtttact
     3001 tcaggtttac ttggagctgt tattggtatt gtgatatcta tacttgagat tcaaactatc
     3061 tttatagttg caggaccaaa aatttcaatg aaaccagatt tagattttaa aacatttatt
     3121 attgttgggt tattgggaat tatagtaact ttaataggtt cgatagtgcc tattataaaa
     3181 gggaagaaaa tgaaactgat agaagaaatt aagtttgaat aattatggag gttaatatga
     3241 aaattacaca taatagttta gctgtagaag caaaaaatat tatcaaagaa tataaaattg
     3301 gaaatacaac aactcgagtg ttaaaagagg tatctttaca agtaatgaaa ggtgaatttg
     3361 tttctatcat gggtcaatct ggttctggta aaagtactct tttatatatt ttaggtggtt
     3421 tagatactcc aacgagcgga aaagtgtata tgaatggagc tgatatatca cattttaatg
     3481 atgagaaaat gagcataata cgtcgtagaa atataggatt cgtatttcaa ttttataact
     3541 taataccaaa tttaaatgtg gaggaaaata taatgcttcc tttgttatta gatggtaaaa
     3601 atttaaaaga ttataaaaat caattagatg aaatcttaga tatagttggt ttaactgata
     3661 gacgtaaaca tacaccacga gagttatctg gtggtcagca acaacgtgtt gctatagctc
     3721 gagctttaat tggaaaacca gagattttat ttgctgatga gcctactgga aatttagata
     3781 gtaaaacagg gatagaaata atagatttac tgaataaaat aaacagagac aatggacaaa
     3841 ctataattat ggtaactcac tcaccagaag cagccaaaag tagtagtcgt actattacag
     3901 tttcagatgg tttaattgta taaggactaa ttattgtatt tgagataagc atttttaatt
     3961 atataaatta gacatatgta aagtggatat aagttaaata ctataaatgt gctataatat
     4021 taaaataaca gataaaagta ctatattttt tattgattat tttaatttta tttgtttatt
     4081 aaatatctaa tgagtattca tactatctct attattaaaa gcagttaatg atatatagag
     4141 ataatatgga ttcttcttac tttaataaat attcacgata ggacaggata taatatatgt
     4201 taaaaaaaga aagtattcat tttataaata aaaataggtc acttatagtt tgtgaagaca
     4261 attttgttta cctcttgcct tgtatgcaat taaaaaattg gatatcgaat tttactatat
     4321 cgtttccaaa tcaaacaata atttctgata attacacgat tattcctcat ggaagtgtaa
     4381 ctttggtgtt tttttatgat gccacagggt tacatagtct attatttggt ccaactacta
     4441 aaccaaaaac agtaggaaat attgctaata gatgtgatgt tatactaatt atagaatttc
     4501 aacctgctgg ttttttcccg cttataggta tacagcaaag tgaacttatt gataaagttg
     4561 ttccattttc tataattaat acttcattag atttagaaat taaaaaaata tttaatgaat
     4621 ctttatctat agataaattg attttaaagt tggaagaatt attgatttct aatattaaaa
     4681 ttgaatactc atatgagttt attttggcaa ttcagttgat tattcaaaac tctggaaata
     4741 tatcttcaca agaaatttct aaaaaagtat tttatagttc taggcattta aacagactct
     4801 ttaatcagta cctagggttg agtatgaagt cattttctcg tttggtacgt attaataaat
     4861 ctataaaact tctaaacaat aataaaactt cattgatgtc tatatgtaat gaattaggat
     4921 tctatgatat accacatttt attaaagatt ttaaaattgt ctgtggaata actcctcaaa
     4981 aataccgagc taatatgtct gatttttaca gtgaaattgc aaaattttaa gttataatac
     5041 ttataacaaa gaaaggatag tgataagatg aaatttggag gagtattgct aattgtcaaa
     5101 gacataaata aatcaaaaaa gttttatgaa gatgttatgg agcaaaaaat cacattagat
     5161 ttaggagaac atgtatcatt tgaaaatgga tttggattac aaagtaatta taaagctata
     5221 attggatatg atttaccaat atatcaacaa tcaaataact ttcagttgta ttttgaggtt
     5281 aatgacatca aacaatggga aagtaagata aatagaatag gaaatattga atttttgcat
     5341 tatataaaag aatacccatg gggtcaaaga acttttaggt tttatgattt tgataaaaat
     5401 attatagaaa tttcagaaag tatggaaagc gtcataaaga ggcttttaaa acaaggttta
     5461 gctttagaag aaatttcaaa acgcactatg tatccagttg aatttattat acaatttcaa
     5521 taattataaa atattaaaat atataatttt ttctttatat tgcaaaaaac aaaggcttgg
     5581 ggcataatag aaccagaaaa acctgaatct caacagcaaa tggctcatat ggatttcgct
     5641 gttaatgatt tagaagaagc agttcaatat gcaattcatt gtggagctac aatcgcagag
     5701 gagcaattta ctgatgactg gagagttatg attgacccag ctggacaccc tttttgctta
     5761 tgtcagatga aatcaattat ggaaagtagc catttttcat tttatagacc aaaatattgt
     5821 ttattaatta caaaaaagca caacagtata taccgttgtg cttttactta ttatacatga
     5881 atacactaaa aaatataaat agtagagctt tatcaataat gataattttt aaaaaatagt
     5941 atttttttat tgaataattt cttagatatt ttaaagtata taaat
//
LOCUS       DAEMHZ010000180         5869 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000180 DAEMHZ010000000
VERSION     DAEMHZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.181"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..752
                     /locus_tag="KPK72_003498"
     CDS             99..752
                     /locus_tag="KPK72_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2490671.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            893..2164
                     /gene="brnQ"
                     /locus_tag="KPK72_003499"
     CDS             893..2164
                     /gene="brnQ"
                     /locus_tag="KPK72_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2490672.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2366..3553
                     /locus_tag="KPK72_003500"
     CDS             2366..3553
                     /locus_tag="KPK72_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG2490673.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3783..5807)
                     /locus_tag="KPK72_003501"
     CDS             complement(3783..5807)
                     /locus_tag="KPK72_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490674.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLIVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         2062 a          703 c          922 g         2182 t
ORIGIN      
        1 atttcgtaaa aactttttaa tatatcaaat atgtatatta agttaatata ttaaaaaaaa
       61 taatatcaaa aataatataa tttatagtgg aggggaaaat gactaaaatt attaaagtgc
      121 aaaaacctaa atttacagga gaattagaaa ttattgaaaa tcttgaagat atacttgaag
      181 atatatttaa tgatgaaaaa atatttaata aaattataga aaatgaagtt ataagtgatt
      241 tagattctgt aagagttagc tttgaatctt gtatatttag aaatgtgtct tttgaaaatt
      301 gtaatttaaa aaagattgat ttacttgatg tggtatttga aaactgcaat ttatcaaata
      361 cattttttga tggaggaagt atatacagag ttgaatttaa aaatagtaaa ttgataggag
      421 ttagatttga tgattgtata cttaaaaacg ttttgttcaa ggatgtttta ggtaagtatt
      481 ctaacttttc attctctaaa tttaagatag tttctatctt agattcaaat tttgaaaatt
      541 ctgtatttca agaagtaaaa ggtgacaatc tagtatttga gtatacaaac ttaaaaaaag
      601 caaactttaa tggaactaaa ctttctggca tagattttac cactaataat attgagggaa
      661 ttgagattgg tatagatgag attagaggag caatttttga tgtgtcacaa gcaattgatt
      721 taacgaaatt gatgggaatt atcataaaat gaaaaaaata ttcataaaat aaattgggaa
      781 aacgttgaca atgtgtgaat ttttaccaga ttgacatttt tctgaaaata aagtaagatt
      841 atcttaatat tcaaaatatt ttcaaagggg gtaatttagt gtgagtaagc agatgagaga
      901 tatttttata gttggttttg ctttattttc tatgttcttt ggtgcaggaa atcttatgtt
      961 tcctccattt ttagggatgg aatccggcaa agactggtta attcctctta ttggatttgt
     1021 tgttgcagat gctggtatag caatgcttgt tattatagca acagcaagat gcaagggaag
     1081 catggatgat gtacttagga gagctggtaa agggttagca agagtaatga gtgtagctgc
     1141 tctagcatgt ttagcacttc ttgtaatacc aagaacttgt gcaacaacat atgaaatggg
     1201 tattatgcct atatttggag accatggacc agtagctaaa gctatatttt caataatctt
     1261 ctttgggtta actttagcat ttactattag gtcttctaag gttatagaca taataggtaa
     1321 gtatttaact ccagcgttat tattagtatt atttgtttta atagtaaagg gtataatttc
     1381 tccagcagga cctatgagtc ctgagcatat gatagataaa aatctatttg gtgaaggtat
     1441 atcacaaggt tatcaaacaa tggacgctct aggtgcagca tctatggcaa ctataatgtt
     1501 gatgtctata atagctaaag gatatacatc agataaagac caaataggaa tgactattaa
     1561 agctggtttt gtagcctgtg tatttctagc agtagtatat ggtggtctta cttatttagg
     1621 tgcaacagtt tcaactttat atgatactag tatacctcaa gcatctttat tagttgaaat
     1681 aactagtata ttgttatatg gtcaagtagg aaaggtaatt ttaggtgtaa ttgtggcact
     1741 tgcctgtctg actacttcta caggtttaac agcatcaata tcaacttatt ttgaaggaat
     1801 aagtaaggcg agttataaac aactggttat aggtatatgt gtagctagta tgcttatatc
     1861 aaatttaggt gttgattcta taatagctat atcagttcca atcctacaaa ctatatatcc
     1921 tgttttatta gcaatagttg ttatggagtt ggctggtaaa catataaaga atgataatgc
     1981 ttttaaaggt gcagcatatg ttacattagt gattagttta ttatcagcta taaatggaat
     2041 gacaggagct gttccattta tacaaagctt gccacttgca ggtttgggct ttaactgggt
     2101 aataccagct atattgggtg gaatagttgg taactttata aaaagcaaca aacaagtagc
     2161 ataataattt gtaattaatt taattttata atatattaat ttttagtact atgggtaggt
     2221 ttaatctaaa cctaatctac ccatagtact ttttagattg gattataata gcatgtatat
     2281 aattttttat aatataattt ttctttgaag tatgataaaa ttagtaatag tgataagaat
     2341 gtacaaattt aggaggtttt aattaatgct tagaaaaata acagcattta tgctatccat
     2401 atgtttaatt attggactta caggttgtag tttacttgta ggcaagaaag ataaagagaa
     2461 aattatagat gattttaaag ttgcagtggt aactcaacca ttatctgaaa ataaagtaca
     2521 atacaatatg gtggaagaga tggcaaaaga atatgaggaa gagaataaaa ttgataaaga
     2581 caaagatggt caaactaaag ttaaacaaac tataaagcat gttgtattgc cagagaattt
     2641 tacttctaat atagatagtg ctattaataa gatagtaaag ttggctgacg ataaagaagt
     2701 tcaggctatt gtagtttcaa ctgaccaagc agggctttta cctgctttac aaaaggtaaa
     2761 ggaaaagcgt ccagaaataa taactatttc tgcccctatg ggagatgata aaaaccaatt
     2821 aagccaattt gttgatgtga atctaggggt tagtgcagag gaaagaggaa aagtattagc
     2881 tgaacgttca aaagaaatgg gagcaaaagc ttttattcat tatgcctcta cagatgattt
     2941 gaaagatgtt aatatagcca aaagattaga aatgattaaa gagacttgca aaaatatagg
     3001 attgccattt gtacaagtta atactccaaa tataaatact gaagaagaca aaaacaaagt
     3061 taagcagttt ttaaatgaag atattgaaaa acaagttaaa aaatatggaa aagatataaa
     3121 tgtatttggt gtaaatgagt atatggatga agttatattg actaaggctt tagaattaaa
     3181 gtatatagtt gcagagcaaa gcaatccatc accaatacaa acatatccta gtgttatggg
     3241 tctaaaaata tctgaaaaag atgctcaaaa ttatgataaa ataaacgaca tgataagtga
     3301 aaaggctaag gcttttggta tgtcaaatag acttggtgga tatcctatgc caatggatgc
     3361 ttttctacca tcattagcaa tttatcttgc tactgaaatg gtgaaacaag atttaacaca
     3421 agaagatgta tgtgacccag attatttaga ggcgtttaca gaattaagat ttggaatcgg
     3481 ttcagaattt actcctttaa cagaggtact ttataattat caatcagtaa ttttaagcca
     3541 attaatttat taatattaag gaagtgtctc aaaatgaact ttttagttta tgaggcactc
     3601 tttttctatt aaatcaaata tatttttatc tttttaacaa ttaaaaagaa gcttatccct
     3661 atgttgagat agcttcgttt ttaatataaa taagttttat tattttataa aaaatttata
     3721 gaaaaataaa aaattataat tatatataaa ttttgataaa gtaaagatga attactatag
     3781 gtttaaatta tattattttc tattattttt atgcctttct cagttattga tgagcctttt
     3841 cttcctttac tagaaattaa gtatccatta cgtgtcaaat attctagcat atttcttact
     3901 tcgtattctg tgacattata ttccataaat ttcattaact ttgtcctccc cattggtttc
     3961 atgtgcaatg attttaggat agaaatgcat atgtttttgt catattggtt taaatcattg
     4021 aatatggaca tattacttga catatgatta ttatttaatt ttgaagatat gttttgaggt
     4081 aaatcattta ttgtaagata attggaaccc atgtaagcca tatattcaac acaatttctc
     4141 agttctctaa tattccccat ccaagtgtat gaatttaaag cttctaaaag actcttatcg
     4201 agttttatgt ttttataggg aagttctttt aaaaatgatt ctaaaagaag aggtatatct
     4261 tctaatctct ttctaagagg aggtaaatct aaagtgaaca tacttatcct ataaaataaa
     4321 tccatcctaa atttattttc tataattaat ttctctaaat ctttatttgt ggcagctaat
     4381 attctcacat caattggaat aatacttgta ccaccaattt ttctaatttc tttttcttgt
     4441 aagactctta aaagtttaac ctgtaaatca tgtggcatat ctcctatctc atctaaaaat
     4501 atagtgccag tgtgagctaa ttcgaataat cctttttttc cacctttttt tgctccagta
     4561 aaagaaccat cttcatatcc aaatagttca ctttcaagta attcacttgg taatgaagca
     4621 caatttattg ctacaaaagg tttatttttt ctatttgaat gattgtgtat tgactgagta
     4681 aaggcttctt tacctgttcc tgtttcacct gttattaata taggattatc tgtaagagcc
     4741 attttttttg cctttttaat acaatctttt ataatagaac tatttccaat aatattatta
     4801 aatttatatt tagctacata acctcttttg acagaatcac ttcttatttt ttgttctaat
     4861 atttctatat tgtttacatc ttttaatgta attaagcaac cctctatatt tttataaaga
     4921 taaaatggtt ttttagaaag tatgaatttt ttatctattt cgtctatgta tataatcata
     4981 ttatcgatat ttaagttctt tgtaatttta tctaagaaac tctttggcat atattccatt
     5041 aaggaaggtg attttataag cttttcatct agttccaaca tattaagtaa agacttattt
     5101 acaaaagtaa ccttgttaaa ggtgttgaaa aatattagac catcacctat ttcatcaatg
     5161 gtcatgtaaa gtatagtttt taatttagat atattatcta atgatgtatt taagaaatca
     5221 tgacttagtg tctccttgga caatttgtat agtttttcta taaatttctc attttttaat
     5281 tttaatacta ttaataatga catatatgta tctaatgaga tttttctcca acctaaatca
     5341 acaatatttt ttatgtattt tggtataaat ctaatctgac ctggtgttat agctgtatct
     5401 aaatcatata tttcatcaca atctggatat gtcggtacaa aatctatatg atttatacca
     5461 attttatata gataagtagc catctcaaat gctgaagttt tattggttgt cataagtaac
     5521 gattttgtac ctggaggcag tttcatcatt ttttcatatc caagttttga aagtgctttc
     5581 gttccatgaa taatttcaat atcttcctta aaatgtttta ttaaaaaatt actagttaaa
     5641 ggtgatagag aaagcactac atctccctct aaattttctt ttattccatc aagtatattt
     5701 tgtgtttcta tgattatttc gtctccaaaa aagtttgtta attcttcttt gtagtggtca
     5761 ctattcaatt tatcatatgt tataaggatt aacttctttt tatccatttc atttctccta
     5821 acaaaatagt atttattttt aaagtattta aagtataagg caagttata
//
LOCUS       DAEMHZ010000181         5630 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000181 DAEMHZ010000000
VERSION     DAEMHZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.182"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(174..410)
                     /locus_tag="KPK72_003502"
     CDS             complement(174..410)
                     /locus_tag="KPK72_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490675.1"
                     /translation="MLRQLRKMRHLTQLELAEKINYNKSYISKLEKGNYKNVTVTTIV
                     DLAIGLEVNYLVIAAIFIMEELKRRKEKNRLAKY"
     gene            922..1083
                     /locus_tag="KPK72_003503"
     CDS             922..1083
                     /locus_tag="KPK72_003503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490676.1"
                     /translation="MDDKKTILRLNNIFTELKNINEDKFDKYKEIVSENKFNIYIEIT
                     NETNKKKEV"
     gene            complement(1092..1451)
                     /locus_tag="KPK72_003504"
     CDS             complement(1092..1451)
                     /locus_tag="KPK72_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490677.1"
                     /translation="MALKDRIKEERVKLNLNQVELAKIFNVTKQTVSNWESGNRIPDT
                     LMLTKLADFFNISVDSLLGRPSLDRKDSNDKFIDELDSPDDIKELIKVFMSLDEESKE
                     KMLKIAQVFIDEEKQNK"
     gene            1640..1867
                     /locus_tag="KPK72_003505"
     CDS             1640..1867
                     /locus_tag="KPK72_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490678.1"
                     /translation="MNTKKLKAYRKLNNVNQSDIANLLNITLTSYSHKENGKTKFTLE
                     EAKTISDFFGLSIEEIFFNNSVNLKFTETVN"
     gene            1907..2350
                     /locus_tag="KPK72_003506"
     CDS             1907..2350
                     /locus_tag="KPK72_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490679.1"
                     /translation="MSYQYQNIYQLGRENTGITQEKASELLDISVESLRAYENDKRIP
                     PNTVVVKMISIYNNNLLGYEHVRRTTEAGVMFLPQLEMKSLSSITLKLHKEIKDYLKK
                     EDDFIDIVEDDVIDEEEEEIWDDVMQELEDIFKSILILKLSKKTK"
     gene            2366..2719
                     /locus_tag="KPK72_003507"
                     /pseudo
     CDS             2366..2719
                     /locus_tag="KPK72_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            2717..3142
                     /locus_tag="KPK72_003508"
                     /pseudo
     CDS             2717..3142
                     /locus_tag="KPK72_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
     gene            3187..3441
                     /locus_tag="KPK72_003509"
     CDS             3187..3441
                     /locus_tag="KPK72_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490680.1"
                     /translation="MSEDIFYGIKNTLDDIAEVQIKNKQGVLKEVGMLISDDENSFIT
                     HCLDEDLIKFYIEDEEVLTIDKDNHLLYMLDALFYNFLDK"
     gene            3469..3912
                     /locus_tag="KPK72_003510"
     CDS             3469..3912
                     /locus_tag="KPK72_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490681.1"
                     /translation="MEKLGNISNFNLDKQEDKSFNELDNISTCFSESIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            4014..4241
                     /locus_tag="KPK72_003511"
     CDS             4014..4241
                     /locus_tag="KPK72_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490682.1"
                     /translation="MKILKVKFPTKILKAGKAIRVTCARFGFESDCIITQSQEFELTV
                     VYFDKELDDLVQSSITIDDAISYDYCIEMLN"
     gene            4254..4571
                     /locus_tag="KPK72_003512"
     CDS             4254..4571
                     /locus_tag="KPK72_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490683.1"
                     /translation="MKSLIIVRNAVEQQLNRANLEINKNEELYTKLRKKEERDVLEEI
                     ELSNALREKSVNERLKIFAESLLEIIDTQIEIKEYEESEDYKIFQLILEELERDRPID
                     VQI"
     gene            4654..5163
                     /locus_tag="KPK72_003513"
     CDS             4654..5163
                     /locus_tag="KPK72_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG2490684.1"
                     /translation="MSTLYELTTDLLEIEEGLTEITGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVIMNIDSDISSIDSEIKRLQELKRVKKNSLDRLKSNIKDCMELLGTKKVETIL
                     GNISIRKSAGSLVIEDEEKIPGIYKTVEQVIKVDKNAIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            5173..>5630
                     /locus_tag="KPK72_003514"
     CDS             5173..>5630
                     /locus_tag="KPK72_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG2490685.1"
                     /translation="METNNIYMKLVNIQSALKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYS"
BASE COUNT         2404 a          535 c          916 g         1775 t
ORIGIN      
        1 ctatgtggta tgtaaataaa cttttggtgt cgcttttata cctagcttat caaggtttta
       61 tattaactat gtggtatgta aatataagag tggttcaaga aaataagtta tttttcaaaa
      121 cgttttatat taactatgtg gtatgtaaat aatttttaaa taatagaaat agactagtat
      181 ttagctagtc tatttttttc ttttcttctt tttaattctt ccattataaa tatagctgct
      241 ataactaaat agttaacttc taaaccaatt gctaggtcaa ctattgtagt aacagttaca
      301 tttttataat tacctttttc taatttggaa atatagcttt tgttataatt aattttctct
      361 gctagctcta attgtgttaa atgtctcatt tttctcagtt gtcttaacat aaatttaaat
      421 caccttttaa tatatttttt agtaaattca gataattata ttatgtgcat ttttgtgggg
      481 aaaagtttcc tattagtcga tttttttgtt ggaaatttgt gctaaaatat aagtaagaaa
      541 taactctatc tagctagaga aaaattaaaa ggtaaaaaat ttatgttaga attaagtatt
      601 taataaattg tggaaaatgt aataaattgt aataaaaaaa atatatttta aaataacaaa
      661 tacaaattaa gaaatgtaaa tgaaaacaaa gaaacataaa gaaacataaa gaaaaataaa
      721 gaaaatattt ttaaaatcaa aattaaaacg agtaaataaa aataaaatat gcaagttgca
      781 gagatattta taggcgtata taataaattt atagattaaa tattacaaaa tataaatata
      841 tttaaaatta aggaaaattt ttcatagctt acgaacgtat gttttggaaa gggaaatact
      901 ttacagggga tggtgtgttc aatggacgat aagaaaacaa ttctaagact taacaatatt
      961 ttcactgagt taaaaaatat taatgaagat aaatttgata aatataagga aatagtaagc
     1021 gaaaataaat ttaatatata tatagaaata accaatgaaa caaataaaaa gaaagaggta
     1081 taacctcttt tttatttatt ttgtttttct tcatctatga aaacctgtgc aatttttaac
     1141 atcttttctt ttgactcttc atctaaactc ataaatactt ttatgagttc ttttatatca
     1201 tcagggctat ctaattcatc aataaattta tcgttactat cttttctatc aagactaggt
     1261 cttcctaaaa gagaatctac agatatattg aaaaaatcag ctaatttagt aagcattaaa
     1321 gtatctggta ttctatttcc actttcccaa ttgcttacag tttgttttgt tacattaaat
     1381 atttttgcca gttcgacttg attcaaatta agtttaactc tttcttcttt tattctatct
     1441 ttaagagcca taatattact ccttccattc acatttctta taaattatta taaactattt
     1501 gttatacttt ttataagata tataacataa agaatacttt ttttaaaaaa atataaattt
     1561 tatgttgaca gtaaactaaa agtagactat tatataaata taaagttaac gtaaagttga
     1621 ctaaaaaaga ggtgataaaa tgaatactaa aaaattaaag gcgtatagaa aactaaataa
     1681 tgttaaccag tctgatatag caaatttact taatataact ttaacttctt attctcataa
     1741 ggagaatggt aaaactaaat ttactcttga agaggcgaag actatttcag atttctttgg
     1801 tctaagtata gaggaaattt tttttaataa tagcgtcaac ttaaagttta ctgaaacggt
     1861 aaattaatct atatattaag tttacattat aaaaggagaa agataaatgt catatcaata
     1921 ccaaaatatc tatcagttag gcagagaaaa tactggtatt acacaagaaa aagcatctga
     1981 gttactagat atatcagtag agagtttgag agcatatgag aatgataaaa ggataccacc
     2041 aaatacagta gtagtaaaga tgatatctat atataataac aatctgctgg gctatgagca
     2101 tgttagaagg actacagaag caggagtaat gtttttacca caacttgaaa tgaaaagtct
     2161 ttcaagtata actttgaaat tacacaaaga aattaaagat tatctaaaaa aggaagatga
     2221 tttcatagat atagttgaag atgatgtaat tgacgaagag gaagaagaaa tttgggatga
     2281 tgttatgcaa gaattagaag atatttttaa atcaatttta attttgaaac tttcaaagaa
     2341 aacaaaatag gaaggagaaa attatatgaa taacttagtt ttaataaatg ataagaaatt
     2401 gcagattaaa gagtttaaag gtgaaagagt tgtaacattt aaagaagttg atttaattca
     2461 tgaaagagcg gaaggaacag cgaaaagaaa tttcactgag aataaaaagt attttataga
     2521 aaatgttgat tattttgagg tcagtacgaa aatcgtacca agcttggagc tatatggttt
     2581 tagtaaattt gccccaaatg ggattttaat aactgaaagt ggttatctaa tgttagtaaa
     2641 atctctaaca gatgatttag cttggaaagt acaaagagaa ttagttaata actatttcag
     2701 agttaaagaa aatgaacaac aacctaaatt accaactaca tataaagaag cactgcaaca
     2761 gttattaata gaagttgaag aaaaagaaca attacaatta gaaaatcaag taatgaaacc
     2821 aaaagcagac tattttgatg ctttagtaga aagaaattta ctaactaata taagagatac
     2881 atcaaaagaa cttggagtaa aagaaaaagt atttgtttta tggttaatag agaagaaata
     2941 ttgttacaga gatttaaaag gaaagataaa accttattct aataagatgc agtactttga
     3001 actgaaagaa tttacaacgc catacggaca ttcagatact caaacattaa taaatccaaa
     3061 aggaagagaa acatttagat tattacttat aaaagatggc ttagtaagag aaagagaatg
     3121 tcagataact ttattaggtt aaaattgaaa gtactttgaa aattaaaaat tatggaggta
     3181 gttacaatga gcgaagatat attctatggt ataaaaaata ctcttgatga catagcagag
     3241 gttcaaatta aaaataaaca aggagtgtta aaagaagttg gaatgttaat aagtgatgat
     3301 gaaaactctt ttattactca ttgtttagat gaagatttaa ttaagtttta catagaagat
     3361 gaagaagtat tgacaataga caaagataat cacttattat acatgcttga tgcactattt
     3421 tataactttc ttgataagta ataaatacag aatattcttg aaatggtggt ggagaaattg
     3481 ggtaatatat ctaactttaa tttagataaa caagaagata aaagctttaa tgaattagat
     3541 aacatatcaa cttgtttttc agaaagtatt cgtaaagttg tagaaatgaa tttaaacaac
     3601 tataaaaata aaatctcaaa gtacttaaat gaaacttcga aaatagaatt attagaacca
     3661 aaagaactaa caattgttat aagtaaaggt tatcccgatt atcttatgtc tgttgaagaa
     3721 gcaagtaaaa gattgaaaat agataaagta tttggatatg agttaataaa aaatgggctt
     3781 ttgaagtcag ttgatatagg ggcaactaaa gtttctagtt atgaattaga tgattttata
     3841 actagaaatc aaggaaaaaa catcaaagaa atgcttagag aaatgaaaga aattagagag
     3901 ggggtgattt agttgaatgt aagagtacta atagcttata tacagttctg taagcaatat
     3961 aataagaaag caagttttga aggacttaaa aaatataata aggggatagt gatatgaaaa
     4021 tacttaaagt aaaatttcct acaaaaattc ttaaagcagg taaagcaatc agagtaacat
     4081 gtgcaagatt tggttttgaa agcgattgta taataactca atcacaagaa tttgaattaa
     4141 cagtagttta ttttgataaa gaattggatg atttagtaca atcgtctata actatagatg
     4201 atgcaataag ctatgattat tgtattgaaa tgctaaatta agggggatta atcatgaaaa
     4261 gtttaattat agtgagaaat gcagtagagc aacaactaaa tagagctaat ttagaaataa
     4321 ataaaaatga ggaactttat acaaaactta gaaaaaaaga agaaagagat gtattagaag
     4381 aaatagagct aagtaatgct ttaagagaaa aaagtgtaaa tgagagatta aaaatatttg
     4441 ctgagtcatt actagaaatt atagatacac aaattgaaat aaaagaatat gaagaaagtg
     4501 aggattacaa gatatttcaa ttaattttag aagaacttga aagagataga cctatagatg
     4561 ttcagatata gaaaagagcc atggcaatgg ctcaattcaa ataactatta aaaaatttaa
     4621 ttaagctaat tatagcataa atggagggaa attatgagta ctttatatga attaactaca
     4681 gatttattag aaatagaaga aggtttaaca gagataacag ggaatgaagc tgaaaaacta
     4741 gaggaaataa aagaaataat aaagcaagag atacaaaata aaaacactag aatagtctca
     4801 gtaataatga atattgacag tgatataagc tctatagatt cagaaattaa gaggttgcaa
     4861 gagttaaaga gagttaaaaa gaatagtctt gataggttaa aaagcaatat aaaagattgt
     4921 atggagttac ttggtactaa gaaggtagaa acaattttag gaaatataag tataagaaaa
     4981 tcggcaggta gcttggtcat agaagatgaa gaaaaaatac ctggtatata taaaacagta
     5041 gagcaagtta taaaagtaga taagaatgct ataaaagact ttattaaaaa aggtcatgag
     5101 gttgaaggtt gtaggattga atatggaact acactaacaa ttccaaaagc taaaaaagag
     5161 taggtgaaga ccatggaaac taataatatt tatatgaagc ttgtaaatat acagagtgct
     5221 ttaaaagctc ctaaaagtca atttaatagc tttggtaaat acaactatag gagttgtgag
     5281 gatatactag aaggtttaaa acctattcta aaggaagaaa aagcattagt tatattggat
     5341 gataatattg ttcagatagg aaatagattt tatgtagaag ctacagcaac tttaatagat
     5401 gcagaaacag gagagaaagt atctacaaag gcattagcta gagaagatga aactaaaaaa
     5461 ggtatggatt tagcacaagt aactggaagt gtatcaagtt atgcaagaaa gtacgcttta
     5521 aatggattat tttgtattga tgatacaaaa gatagtgatg caaccaataa acatggaaat
     5581 gagcagaaaa aaaaagaagt taatgagagt gaattaaata cactatattc
//
LOCUS       DAEMHZ010000182         5616 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000182 DAEMHZ010000000
VERSION     DAEMHZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.183"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            74..3022
                     /locus_tag="KPK72_003515"
     CDS             74..3022
                     /locus_tag="KPK72_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490686.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3000..3464
                     /locus_tag="KPK72_003516"
     CDS             3000..3464
                     /locus_tag="KPK72_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2490687.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3577..3879
                     /locus_tag="KPK72_003517"
     CDS             3577..3879
                     /locus_tag="KPK72_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2490688.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3941..5305
                     /locus_tag="KPK72_003518"
     CDS             3941..5305
                     /locus_tag="KPK72_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2490689.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIITLLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVIFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5340..>5616
                     /locus_tag="KPK72_003519"
     CDS             5340..>5616
                     /locus_tag="KPK72_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490690.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKI"
BASE COUNT         2279 a          610 c         1012 g         1715 t
ORIGIN      
        1 aataataagt acaaaaaaaa caatatatgg catataagtt gctatataaa taataaggag
       61 ggaacaatta taaatgaggc aaagaataaa aaacttaatt gaaaatgaag ataagaaaaa
      121 tcctttaaca gatgaagcga tttcaagtag gttaaatatt cgaagagaag atgttacctt
      181 tttgagaaat gagttaaaaa tagaagattc aagacaaaga agaaaagttg ttttaattaa
      241 agctataaaa gatattttaa aagaagaaaa aactataaat aaaaatgata taacaagaag
      301 attaaacagc attgggttta cagtatcaag atttacagtc atacaatact taaaagagat
      361 tcaagattct ggtgacctaa aacttgataa agagagtgat gataaaaaag aagaaataat
      421 caataataga gaaaataaag atatagcatt tgataaactc ataggtagta gtggtagctt
      481 aagtacaatt ataaaactag ccaaagcagc aattctttat ccacctcatg gtcttcatac
      541 tattattatg gggccaactg gtgttggaaa gagtgagtta gcagagtgta tgtataaatt
      601 tgcagtagaa tcatccagat tccctaagaa tgcaccattt atagttttta atgcagcaga
      661 ttattcagag aatccacagc ttttgctatc acagttattt ggacacgtta aaggtgcttt
      721 tacaggagct gatgagaaca aagaaggatt agtatcaaag gcagatggag gaatattgtt
      781 tatagatgaa atacatagat tacctcatga aggtcaagaa atactatttc aactgattga
      841 taaaggtatg gtcagaaagt taggagaaac aagactaaca cataaaatag atgtaatgat
      901 tatttcagca acttctgagc caatagattc tcatttactt aatacattta aaagacgtat
      961 acctgtaaca attgaaatac cagaattagt tgcaagacca ttaaatgaga ggtttgacat
     1021 tataaataat ttcttcttaa tagaagctca aagaatggga gcaaacatac atataaagtc
     1081 agatgtatta aaagctctta tgctttacga ttgtataggt aatgtaggtc aacttagaag
     1141 tgatattcaa gtagcttgtg ctaggggact attaaaccaa cttacaaaca aactaaaaga
     1201 agtaaatata agcatatcag atgtacctgg atatgtaaaa caaggtctta taaaaatcag
     1261 aaattgtaga ggaaaaatag aaaattatgt agatggggat ttaattatag attcaaatct
     1321 tcaaggtgag atagaaaaga aaaatgaaga tatatatact tttccagatg aaatatacaa
     1381 acttactgaa agaagacata ttgatttatt ggaacaaggt ttagattttg atgttataaa
     1441 tagaatcatt ggaggagaat tagaaagttg cattcaaaaa tacataaaac aagttaaaaa
     1501 agtttctatt agtaacaatc ttccagatat aagtccaatt gttggaaaat atatcgtaga
     1561 gctaacttat gaagtaatca aaatagcatc acaataccta agttcactag aaccgaatct
     1621 acatttatgt ttagcagttc acctaaaagc agcttttgag agattaaaag aaggaaaagt
     1681 aatatcaaat gctcatttga aagaaataat aggaaattat ccactagaat attctgtagc
     1741 agaaaaggta gctcaatatt taaaggaaag atataacata aacttaccaa aagaagaaat
     1801 aggtataata gcaatatatt ttaaaatgag tagtagaaga gatatgtctg aacaaaaaaa
     1861 gataggcgta cttcttatgg ctcacggaag agtagcatct tctgtatgtg aggttacaaa
     1921 taaactatta ggaattaatc acgcaaggta tttagatatg ccatttgaga agaagccgga
     1981 agaaatgatt gaagaagcta ctacaataat aaaagatatt gatgaaggta gaggtgtgat
     2041 tatattagta gatatgggtt cactttcata ttttggagat attatttctg gaaagactgg
     2101 aattaatata aggacaatat ctagatttga tacattgctt tctatagagg ttatacgaag
     2161 agcagtttta ccagatttaa caatagacga gatagttgat gaagttaaag gtatagagcc
     2221 acaagtaaaa gaaaaggtta gtaaatgtaa caatacaaaa gtagatttta gaaaacctat
     2281 aattttaaca ttatgtatta ctggattagg gggagcttgt aagataaaaa aaatcttaga
     2341 ggaaaatatt aaaagtataa ctgaaaaggt tgatatagaa cctataggta tgattgaata
     2401 tccaagtctt gatttagaga taaaacaaat aagcaatgat aaaaatgtaa tcgccatagt
     2461 tggaacaatt gaccctcaga tagcaaatat cccttttttt agtctagtag acataaatga
     2521 taaagcgaag ttaaatcaaa taaataacct aattgataca aatcaaattg gtgaaacaaa
     2581 taaaaaatat gaattatcag atttaataca taaagaagat atatttgtgg atttacaggt
     2641 taaatcaaaa gaagaagttc taaaatatat gagtaaaaaa cttagagaga aaggttatgt
     2701 gacaaaaaat ttctataaaa aagtagtgga aagagataac ttatttccaa ctgaattatc
     2761 agatgaaata gcaatacctc acacagatgg aatagatgta attaggccag ctataagtat
     2821 agccatctta aagaagccta ttgtgtggag taaaagtaaa gtaaaaataa tacttttatt
     2881 agctgtagac caaaagtgtt ttaatccatt aagcactcta ctctcattta tagaaacaga
     2941 tgaatttaag aagataaaaa acattaaaga tagagatttt attagggaga tgatattaga
     3001 tggagtcaat gaaaataatt agtagcaatt taatttttaa aaatattgaa gtgtctaaca
     3061 atgaagatgc attaaagttt ttgggacaac gtttgtttga cgaacagtat gtaaaagaaa
     3121 gctacataca agctgttgta gcaagagaaa aaaagtatgc tacagggtta ccaacagaaa
     3181 tatacggagt ggcaatacca catacagata tagtacatgt aaatgagcca ggaatagcaa
     3241 ttggaatact gaataaacct gtaaaattta taatgatggg tacagacgat actgaaattg
     3301 atgtaaaagt tgtatttatg ttagcagtga aagagccaca agaacaattg caactattag
     3361 aaaaacttat gactatattt caagataaga atatactaaa taatattgtt gacttaagtg
     3421 aagaaagtgt gagtgattta ttaaactcta aattgaaaaa ttaaacatgt aaagtatatt
     3481 aaaaatgtaa aattaaacat gcaaagtatg ttaaaaaata taaaaagatt attaaagatt
     3541 tctaactata gatatcttta aaataaaata atttcaatgg gggtgactaa tgtggctaaa
     3601 aagatattag ttgcatgtgg aacaggagta tgtacttcaa caattgctat aaacaaatta
     3661 aagaaagcac ttcaagacat tggtaaattg gatatggtta atattacaca atgtaaagta
     3721 gtagaagttg catcaaaagc acctgattat gatttaataa tttgtactac acaagtatct
     3781 agtagtataa aaacaccagt gataaatgga ttaccttttt taactggtgt cggaatggat
     3841 aaattaatca atgatgtatt agaagagtta gaattataat taaaaaaatt aaggaacatg
     3901 gtctctaaaa tcataagttt tagagactta aaattaaata atggagggtc tttatatgga
     3961 gatattaaat ttcattgttg gtctaggtgc acaagtaatg atgccaataa taatatgtat
     4021 ttttggtttg atattaggta caaaactagg aaaatcttta agagctggtt taactgtggg
     4081 agttgggttt ataggtctta acactattat aactttgcta acagataatt taggtccagc
     4141 tacacaacaa atggttaaga atttagggct aagtttatca ataatagatg taggatggcc
     4201 agcagcatca gctatagctt ttgcatcaac agtaggggct ttaattattc caataggtct
     4261 tgtggtaaat atagttatgc taattactaa tactactcaa actgttgatg tagacatatg
     4321 ggattactgg cattttgctt ttacaggagc tttagtggca ggggctactc aaagtgtaat
     4381 gtggggagta tttgcagcag tagcaaatat ggtaattgta ttggtaatgg cagatttaac
     4441 tgctccaggt atagaggaat atttaggtat gccaggtata tctcttcctc atggatttac
     4501 acaggcattt gttccaattg ctatagtagt aaataaatta ttagatctaa taccaggtat
     4561 aaataagatt gagataaatg ctgatacatt gcagaaaaaa tttggattat ttggagaacc
     4621 tttaataatg gggtctgtaa taggtgtaat aattggtatt gctgcgaaat atgatataaa
     4681 aggaatatta caattaggtg taactatggg agctgtatta atattaattc caaagatggc
     4741 agcattatta atggaaggat tattaccagt atcagaagct gcacaggaat ttattgagaa
     4801 gagatttaaa aacagaggaa agatttatat aggcttagat tcagcagtag gaataggtca
     4861 tcctgttaca ttatcagttg cactagtgtt ggttcctttg actatactta tagcagctat
     4921 attgccagga aataaagttt taccatttgc agatttagca gtaattccat ttgcacttgt
     4981 attaatcgta ccaattacaa agggaaatgt gtttagaaca ttaataatag gaataattat
     5041 aattacttct ggattattaa tcgcaactaa cttagctcca ttatttaccc aaatggcttt
     5101 aaatgctagt tttaaaatgc ctgaaggagc aactatgatt tcaagtattt gtgatggtgc
     5161 gaatccatta agttgggtat ttgtaaaagt tatgaactat aaagttattg gaggagtaat
     5221 ttttggagtt atagctttag gaatggctat ttacaatagg aatagaataa taaatgaaaa
     5281 taaaaagcta tctatagaag aataaggtta aaacaatttt attaaggagt gtataggtta
     5341 tgaaaaaata taaatatgat tctaaaaggt acttactcag tgttacaata ccaggatttt
     5401 ttatgatttt aatattgatt tatgcactgt ttaataacta tacaaatttt aatttgaata
     5461 tatatagttt gttaataatt gtctgtactt atggtatatt caatactttt atatcatcaa
     5521 gtaatcctaa aaaaattatt attgatagca aatgtataca ttttacctca tttatgacta
     5581 cacataagta tgaaattaaa gatatagaaa agataa
//
LOCUS       DAEMHZ010000183         5551 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000183 DAEMHZ010000000
VERSION     DAEMHZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.184"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(163..1362)
                     /locus_tag="KPK72_003520"
     CDS             complement(163..1362)
                     /locus_tag="KPK72_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG2490691.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            1481..2368
                     /locus_tag="KPK72_003521"
     CDS             1481..2368
                     /locus_tag="KPK72_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2490692.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            2558..3115
                     /locus_tag="KPK72_003522"
     CDS             2558..3115
                     /locus_tag="KPK72_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490693.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(3354..4214)
                     /locus_tag="KPK72_003523"
     CDS             complement(3354..4214)
                     /locus_tag="KPK72_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2490694.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            4707..>5551
                     /locus_tag="KPK72_003524"
     CDS             4707..>5551
                     /locus_tag="KPK72_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2490695.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLKKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPE"
BASE COUNT         2062 a          754 c          717 g         2018 t
ORIGIN      
        1 tctgacaatc ttgtttaaat tatttaaata tatcttatct gtgaacatta aataaacctc
       61 ttaataaata taactcttga aaagtcatat ctattaagag gttttaataa attttcatgt
      121 taatatatat aatccaaaaa aattaaatct tattaaaaca tactattttg tatagttatc
      181 gaaatatcct tgtatgtaaa taataggagt acccttatct ccacttccag atgttaaatc
      241 tgctaaagaa cctataaggt ctgttaatct tcttggagta gtaccttgag aaaccatgtt
      301 tcctgttaaa tcatcagatt tattatcttt ttcaactatg tattttgaaa ttgcttcttt
      361 taactcatca ccagataagt cagcaaaatc attatctgct aagtatttta attttacttc
      421 gtttggagtt ccctctagcc cttttgtata agcaggagat actactgggt cagctaattc
      481 ccatatttta cctacagggt ctttaaaagc tccatctcca taaaccatta cttctacatc
      541 tttccctgtt atttctttta tattaccttg gattttgttt acaacttcat cacaatttat
      601 agggaataat tttactgttt cttctgtagc tttgtttgaa cctaaaagtc catactggtc
      661 attgtaacca ctaccatcta cacttgctgt cattatatca tcaagtgaat atactttttt
      721 agctccattt tgtcttaaaa ttctctttgt tctttgtctt gtatgtatat cacaagttaa
      781 aatacttgtt gtatagttaa gaatagtttt tggattgttt gcaaatacta tttctacctc
      841 tgcaccacaa tccttgatta aattcttata ataatcaaca taatctacac cagtaaatgt
      901 atgcttgtta tatccaaata gttctctgta tttttcttct gttaaaacat ctgaccaagg
      961 atttattccc ttatcatcaa gttcatcaat acttattaag tgatttccaa cttcatcaga
     1021 agggtagctt aacattaata cgacttttct acatcctttt gcaattcctt ttaaacatat
     1081 agcaaaacga tttctactta aaattgggaa tataacccca acagtatcat ctccaaattt
     1141 atcttttaca tcttttgcaa tattgtcaac gtgagcataa tttccctgtg ctctagctac
     1201 aacagcttct gtaacggcaa ttacatcttt gtccctaact tcaaagtttt cactttttga
     1261 cgcatttaaa acagaatcaa caactatttt aactaagtca tctccttgtc ttataatagg
     1321 tgctctaacc cctcttgaaa cagttccaac taatctatcc attatctaaa atcctcctaa
     1381 agaattaata ttgtcatatt agtaccatgt atattataat tcaaatataa ttataagtaa
     1441 aataaataat actaatattt gacataagga gtaattatat atgaatataa aacttgaact
     1501 ttataaggtt tttaatgcag ttgtaaataa taagagcttt tctcttgctg ctaaggaatt
     1561 gtttatgtca cagccagctg taagccagtc aataaagcaa ttagaagaac aattagatac
     1621 tttattattt tatagaaaca ataagggagt aaaattaact ccagagggga aaattctaag
     1681 tgaacatgtc actactgcat taaagttaat atcatcagga gaagatagaa taaataaatt
     1741 taaaaaatta gaatacggtt ctctgaaaat aggagtagga gatacagctg cacgtttttt
     1801 cttgttaaaa tatttagaaa tttttcataa gaagtatcca catatacatg tatctacaat
     1861 aaatagaacg agtagagaac ttatatcttt actcaaagat gggaatatag atatagcaat
     1921 tataaacatg ccaatagaag atgatacact aaatattgtt gagtgtattg aaatacatga
     1981 tatatttgta tgtgcaaatg attacataga atacaaaggt aggaaaatat cattggaaga
     2041 attaaacaca ttgcctttaa taatgctaga aaataaagca aattcaagac tttatgtaaa
     2101 tgaatatttt ttatcaaaag gtataaaact aaacccagat atagaattgg gttctcatga
     2161 attactttta gaatttgcgt atataaatct tggagtgtct tgtgttatag aagaatttag
     2221 catagattat ttggaaaatg agaaattgtt taaattagat ataaaagagc caatacctaa
     2281 gagaaatata ggttactgcc atttaaaaga tatatcttta tccttagcaa ccaaagaatt
     2341 tttaagcatg atatctaata atatataatt tagaatacat atttcacaag taatttaata
     2401 taaaaataga gataaattcg taaacctgaa agattcatat agtaattagt ttttattaaa
     2461 aataaaaata aggttaattt gagaacattt atataattaa tatcgtattt taaatatgaa
     2521 cagtatattt agattaatgt ataaaggggg attagaaatg tctgattctg aatatcctca
     2581 aatgaaaacc ttagaaacta gaagttgtat actaagacct gcatcattag atgatgttga
     2641 agattttttt gactgttata aaaataaaat agtagtaaaa catttaccat ttagtgagca
     2701 taaatcttta gaagatacaa gaaagtttat aaaatcattt tttctaaatc catataaaaa
     2761 agggaaaatt ggtcactttg ctatagtata taaaagagac aataaagtta ttgggaatat
     2821 ggggtttaat aatataaatc ctaaagcatt agaagcagaa ataggtatat gtataaatcc
     2881 gagctattgg ggacatgatt ttgctactga gataacaaaa agagtaatac agtatggatt
     2941 tagagaacta aacttaaata aaataattgc aataacctat gaagaaaacc aaaattctac
     3001 aaaatcatta gatttattag gatttaaatg tacaggaaag tatatcaaaa aaattcatac
     3061 tggaaatact gtaaaaaatg ttccttgtta tcaatatgaa ttgaaaaaac aatagatata
     3121 atcatttaat atctatgctg gttatttagt aatgattgtt ttctaaataa aatttcaata
     3181 aagtggttgc ctcaaaatat agataatcct gtttttattg ttaaaatact aaaaatgcat
     3241 ttgattttat acaaaaaaga gtgtctcatg aactaaaaag tttatgagac actctttttg
     3301 aataaatcgt attttaatta aaatagtata tatacttaaa atattaattt ttattagttc
     3361 tctaaatttt taaatccttc tccaagaacc tcatgtacat ctgtaacaat aacaaatgca
     3421 gtagggtcag ttcttttaac taatctcttt agataaagtt cttgtttttt agaaactacg
     3481 actaatagaa cttctttatt ttctctagtg tatccacctt taccatcaag aatagtaaga
     3541 cctctatcaa gttcttcagt tatagcttgt cttaaacttt caggctcatc agagataata
     3601 aaaaatgctt ttgaatggtc aaatccttcc attatcatat ctgctatttt tattaataca
     3661 caaagtgcta taccagagta aagagcagtt tctaaactac gatttactat accagaagat
     3721 ataacaacca ttccatctag gcatgacatg aattttgtag cttttattcc tgggaatttc
     3781 ttactaagta tgagtgcaat taagtctgtt cctccagtag aagcatctga tttaaacatt
     3841 attcctaaac ctataccaac taggattcct cctgcaatag aagacaataa taagtcatta
     3901 gtaaatctta gttttgataa tgggtctgtt aattgaacta tagctgaaaa tacaactgtt
     3961 ccaaataaag tttttaatga attttttgta cccataattc taaaagcaag tattactaat
     4021 gggacaccta taattaacat gatagctgat actggaagtc ctgtaacctt gtttagtact
     4081 aatgatagac cactaagtcc tccaggagca attgtatgtg gttttaaaaa catatttata
     4141 ccaatactca ttgatataca accaaaaaat agtccaagca tttcaagtaa aattggcgac
     4201 ttttgttttt tcatataaac acctccatga acttgtcaca atttaatatt ataacatatt
     4261 tgaccagttt atattatata aaatttcttg atataagcaa tttcaagtat ttcattattc
     4321 ttaatatttt tgaattttta tatagtgttt tcatatcttt actgatgttg ttacactcct
     4381 atgaagtttt tatatattat aattttttta ttcttttata aagtatcccc tataaagatg
     4441 gttctatgaa aattttctct atactgttta acgtatatag agattttgtg tataattata
     4501 aggtatttaa tggaaaaatt tatcttactt ttatatgtta tcaaaaggat ttattaaata
     4561 caacagacag cttgataaca tataattata taaatataat ttatgtagat agttgtattt
     4621 ttaaataaat ttttacatat caataattat tgtctatttt atataagcaa caatcaaaat
     4681 aaaagtttaa gaaaagagtt gattaaatgc ataaacctag ttataaaaca gtatttcatt
     4741 catacattat ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct
     4801 tatcacttat tactattcag aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa
     4861 aatttacaga aaactttaat gaacaaatcg tatttataga taataagcca caagtaaaac
     4921 agtctggatt aaaaaaactt cgagaaaatc aattgtggtt gcaaatttta gattctagtg
     4981 gaaatagagt ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg
     5041 aattattagc attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa
     5101 ttaaaaataa taaaaatgac tatatttata ttatatattt tccattaaat atatctaaag
     5161 tgaatatgtt ttttgatgga ggtaaattta taggtggtaa gtcaattgta ttttcaatca
     5221 tagggattat atttttaata attttggttt ctggaattgc ttatggttac tgggtaacaa
     5281 agtgcatatc aaatattaca tctgctgtta gcgatatttc aaaacgaagc tatcttccag
     5341 ttaatattaa aggtgttttt gctgatatat ataatagttt aaatatttta aatgaagaaa
     5401 ttcataaaag tgatgagata caaaataaaa ctgatattat gagagaagag tggatttcaa
     5461 atataactca tgatttaaaa actccacttt caccaataaa agggtatgca gagcttctta
     5521 cagatagtag tactaattat tcacctgaaa a
//
LOCUS       DAEMHZ010000184         5523 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000184 DAEMHZ010000000
VERSION     DAEMHZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.185"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(295..670)
                     /locus_tag="KPK72_003525"
                     /pseudo
     CDS             complement(295..670)
                     /locus_tag="KPK72_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(775..1152)
                     /locus_tag="KPK72_003526"
     CDS             complement(775..1152)
                     /locus_tag="KPK72_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2490696.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            1957..2445
                     /locus_tag="KPK72_003527"
     CDS             1957..2445
                     /locus_tag="KPK72_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2490697.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            2834..3004
                     /locus_tag="KPK72_003528"
     CDS             2834..3004
                     /locus_tag="KPK72_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490698.1"
                     /translation="MHVYQPKTSEYLLNRSAKSYLSSIVFLFDYITKNESRYAFFIIN
                     FPFLSLDIFYKF"
     gene            complement(3481..3669)
                     /locus_tag="KPK72_003529"
     CDS             complement(3481..3669)
                     /locus_tag="KPK72_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2490699.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            3831..4181
                     /locus_tag="KPK72_003530"
     CDS             3831..4181
                     /locus_tag="KPK72_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG2490700.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            4183..4383
                     /locus_tag="KPK72_003531"
     CDS             4183..4383
                     /locus_tag="KPK72_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490701.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     regulatory      4563..4652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4707..4883
                     /locus_tag="KPK72_003532"
     CDS             4707..4883
                     /locus_tag="KPK72_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490702.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     gene            5108..5308
                     /locus_tag="KPK72_003533"
     CDS             5108..5308
                     /locus_tag="KPK72_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2490703.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT         1998 a          624 c          657 g         2244 t
ORIGIN      
        1 tgagataaat tgaggtttat ttgaataaat tgatttgaat tatgttattt tcagattact
       61 tttatttaag tattttgatt ggattcagct gaattaatgt atattaattc ttattgaaca
      121 gtttttattt ttatacatac tatatactat acatattcta ctaaaattta tataaatatt
      181 agacaaacct attttatttt cttaacatgt tcagaataat aaacaaaatg aaggtagaaa
      241 aagttataat ttcatacctt ctttttattt attatgtatc aatttgtcat tttcttattt
      301 caaattctta aaatattctt ctaataaatt taaaatatca ccatctactt catcatcctt
      361 actacatagt gctgacatta aacttaatat tgaattttta tgtgtattct ttaaaaattc
      421 ttttgtttaa aatctctgat attatttttc ttttactaat acattatagt gtgtacactt
      481 atctattttc tccgtttcta aaaactctct tctttagtaa tctttttaat actgtaaata
      541 tagttgtata tttccattca tatttttgtt ccatagactt ggtgatttct cttgatatta
      601 ataccttttc tgattcccaa atatatctca tatcttttaa ttccgcttct ggtaatttac
      661 taattttcat agtatacatc cctttaccaa tttatgtata tgtcaaattc tatatcaata
      721 aagttttaca aacaaaggaa ttaaggtaaa taattacatt cgatttttac tatatcattc
      781 ttttaagttt ctataatact catctaatag gtctaacttc tctttactta catttttatg
      841 tatagtggta agtgaatgca ttatttttat gcttttacta gattttagta cctttttctt
      901 aaaagtataa tattctttat tatctattat tattttataa taagattgaa aatttataat
      961 atatctagcc aaataccttt tatcaactag cctttttagt agtattttta ttgtactttt
     1021 atgccatcca taaatctgtt ttatttcttt tacaatttct tttttaggca ttagagtgtc
     1081 ttttttccat aaatactcca ttactattaa ttctgctctt aataattttt ctattaatat
     1141 ttcttctttc aatttatgtc acttccttaa ttcttctaac ttaaattaat aataggagct
     1201 ttttaagaca aaaactagag gtaagtttta ccaaatattt tacgtattta gtctgttttt
     1261 ttaaacgtaa aataatattc atatatataa atttgtttaa tttgcaaaca atgctaccaa
     1321 acaataaata gctctattgt attattttag ttgtagaata gcactaaatc gctagaatca
     1381 aagaaattta gtagcacaaa gctatatagg ctaatgctta taaaataaat tataagttat
     1441 gttaacaagt tactaaaagc atattttatt tttgttttta tgaaagtttt ttaggtatgt
     1501 aataatatat atcttttaaa atatgtattc atgtatacat attttgactt attatactga
     1561 tagttcatat aattattttg attttataga tacacagtta gatgatttta tactagagca
     1621 taaaaaaaat gtagtataat cttgtttata tcaagttatg gtattggtaa gtaagtataa
     1681 ttaaatagac attattttct gagtcctctt tttattttca tctgctttta aataatatta
     1741 ttgacattga tatgactgta gttttcttta ttttaatcca tttcttatat cttttctaac
     1801 aagaaatgtc attttaaatc atttccataa attgtaaaat aatagtatat ttgtcataga
     1861 aagtgatttt tttgtctata tatggtaaaa tatagaaagt ggaaatcaac aaaataaaaa
     1921 tttatatatt attaatttta tatggagggg aaataaatga aaaataaaaa aggatttact
     1981 ctagtggaat tattagtagt aattgctata ataggaatat tggcaatagt agcacttcca
     2041 gcattattta aaaatataga aaaagcaaag atagctaaac ttgaagctga tataagtgca
     2101 ataaaaagtg cgtctcttag ctactatgca gatgaatcaa aatatactga aggaaacata
     2161 atatggtgga ctaaaaaaga tggaaaaata acagtaaact ctggtattgg tgatgaagac
     2221 cctttggcac ataaaataga aaatttaggc atgccttata atggttcgta cactttagtg
     2281 tcatctaatg gtagtgaaga atacttagaa ttaaacataa ttatagatgg agaaataagt
     2341 aaaagtggtc tagataaatt agaagaagat tatggtagtt caataaaaat accaaatgat
     2401 aaaaatatga taataacttt tttatctaat aaatcagaca attaaaaata aatatatagg
     2461 tctgataatt attgttaaag taaaaagagt tggttataaa aatgaaaaaa gcactcctca
     2521 agaatagaat gcttagttat aactacagaa gtgtattggc atacacttct attttttatt
     2581 atatcacata atttaaaaat acctattcaa gttgttttta aattatgttg agtaatattc
     2641 ttgatagttc atacattaat gaatttcaaa aaaataagta ctctttataa taaaaagtac
     2701 tttagtgtat gttaatgtcc tatctaataa aattgtaaca tgccttatac tttagtatga
     2761 taattttcat tttcttcatt gttgcaactt atactatagt tttgctaatt tatctgcaaa
     2821 cacaacttct ttattgcatg tgtatcaacc aaaaacatca gaatacttac ttaatagatc
     2881 tgcaaaatcg tatctttcat ctatagtatt cttatttgac tatataacaa aaaatgaatc
     2941 gagatatgcc ttttttataa ttaattttcc atttttatca ttagatatat tttataaatt
     3001 ctagcatatc atttgttatt ttctgatatg ctattgatgt tttaaatctt ctatttgttt
     3061 tattattttt tacttttcca attatacatt tataggagtt atatttatgt gatttatgaa
     3121 aaaatgctat gcatttaagt tctaattttt tgataataca tctaactttc ttttgattaa
     3181 taatgatacc actgtttttt aatgtggctt ccctgaggca cacatctttt ttaaaaactc
     3241 aagctcatct tttaaaaatt tattttcata ttctaattct tgttctcttg ttaatttatt
     3301 ttttctttag tgtttttgtt tttattcatt atggtcttcc tttcattttt gaaaaggcat
     3361 caacaccact atgtaaaaat ttttattttc atgttactat aactgcgcta ttgtttattc
     3421 caaaatactt agcgatttat tgtacactat atttagttat catcatatag tttataacat
     3481 ctaacttgaa ccatatagta tagaaaattt ttttattttt agttcactct ctcctaatat
     3541 attaacccat ctttcaactt gagaatggtc ataaactcca tattgtttta ctattaaaat
     3601 atctcttcct tctctattca aataaaattt agctactttt aatttgaaat taaaatcata
     3661 tttagtcata cataaacatc tcaagcatta gatttttgct ctaacaatta tggtgcagta
     3721 caaatattgt ataatactga ttttctattg acaaaagaat ataaaagaga tatatttgta
     3781 tcataaagat ataaatatat ctcttttaaa attactaagg aggataaaaa atgattcaga
     3841 aaaaattttt tatgggtttt tgtggtttcc ttggattttt aagtcttaga tatttttcat
     3901 ctggcaatgt tgctgacctt acatatattg gattttttgc ttttttctca aactttatta
     3961 tagcaaaaat aaatggtgat aaagcagatg aaaggtatgt acaagatgaa aaagcagcta
     4021 tggcatttac aggacagctt gctattatag aattattcat tttatggtgt attactatag
     4081 tgtctaggaa tatagaatta atgtgtgttt tactatcaat tacatatgca atcactttaa
     4141 atgtttatgc tataaaactt tatattttag aggaaaaata atatgcccaa attcatttgt
     4201 aacctaaaaa aatatagaca attaaaagaa ttaactcaag aacaattagc agaaaaagta
     4261 ggagttagaa gagaaaccat tatgcgttta gaagcaggaa agtataatcc ttctttgaaa
     4321 ttagcaatag atatatcaaa aatagtagat acacctattg atgaattgtt tatttttgat
     4381 tagtttttgc attattaaaa atgtgtttta aatacaaact taaattcaat ttttaagtca
     4441 ctcctgacag aggggctttt tattttttca aataatttaa cgttcatata taaatttatt
     4501 aatttttcag ctagatatgt tgaataataa ctaattctat tgtattatta atctgtagaa
     4561 taaaactaaa tcggcaaaac tagagaaatt tagtgacgca aagctatagg gactaacgct
     4621 tataaaataa gttatgtcag ccagttgcca aaaagatatt ttctttttgt ttttttatga
     4681 aagtttttta gggggataaa tacattatgt ttagagataa aatggataaa tgtacacata
     4741 tgttaactgc ttatattggt agttcatatg attattgtga ttttatagat acacagttag
     4801 atgattttat attagagtac ggagaaaaag ttgtggaatc ttgcttgcat caagtgatgg
     4861 tattggtaag taagtataat tagacagatt tttttctaga aaaccctttt tagttttatc
     4921 tacatttaca ttaatatatt ggtaatataa aatgtttaaa tttattttca tattaagaat
     4981 aaataaaaat gttaactaaa aattataatt aaatagacat ttatgttaat ctatgataca
     5041 atagtatttg tggtaataca caagtaaatc aagtatatct tgatataggt taataggagt
     5101 atttttaatg aataatggaa tagtaaaatg gtttaataat gaaaaaggat ttggatttat
     5161 atcaatggaa agtggagatg acgtatttgc tcatttctca gctatacaaa cttcaggatt
     5221 taaatcatta gaagaaggtc aaaaagtaag ctttgatata gttaaaggcg ctagaggtcc
     5281 tcaagcagaa aatataacta tattataata tatagatttt tcggaaagac tcttttaaga
     5341 gtcttttttt tatttacgca ttttgtaaaa aaggaaaaaa taacttcaac aagaaaatac
     5401 ttaaaaataa tacttagtct aaatactttt tataaaataa atctgacaaa aatatattta
     5461 taaagtatgt acttaaacct acaagtactc ttttaattag aatatatagt aatataataa
     5521 ttt
//
LOCUS       DAEMHZ010000185         5514 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000185 DAEMHZ010000000
VERSION     DAEMHZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.186"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1235)
                     /locus_tag="KPK72_003534"
     CDS             complement(<1..1235)
                     /locus_tag="KPK72_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2490704.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKND"
     gene            complement(1457..2206)
                     /locus_tag="KPK72_003535"
     CDS             complement(1457..2206)
                     /locus_tag="KPK72_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490705.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILDLFNTSFKWISENFLDILLLVVVMFISYSSFILL
                     LYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYHLYQSP
                     SFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(2200..2925)
                     /locus_tag="KPK72_003536"
     CDS             complement(2200..2925)
                     /locus_tag="KPK72_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490706.1"
                     /translation="MDKNINNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(2927..3295)
                     /locus_tag="KPK72_003537"
     CDS             complement(2927..3295)
                     /locus_tag="KPK72_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2490707.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(3471..5015)
                     /locus_tag="KPK72_003538"
     CDS             complement(3471..5015)
                     /locus_tag="KPK72_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2490708.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         1973 a          914 c          499 g         2128 t
ORIGIN      
        1 tcatcatttt ttacccctat atgagatgat actatagtta taatacttag tactataaat
       61 actacaaaac cttttttata tccatcaact atattatatg aatttacttg taaagagata
      121 gctataatca aattaataat tccacttcct ataaattcaa aagtattaat aacactagtt
      181 gccagtccag aataaatttc tttattttta tacttggcat cattaaaaac ctgtagatgt
      241 gacataacta tacatcccat aataaagaat gatattggta aaactataat tggtggtttt
      301 acctggtata caactacaat tacaatccat ataaacaaat ctatcattgc cccaaatttt
      361 attatattaa atttgctaga ccttatttta gcaaatacaa aatccataat tattgaacca
      421 aatataaatc catatgtaaa aaatgatact ataaacgcag aaaaactctt actcacacca
      481 taaacgtcca ttatgtatct tactccccac aagcttataa atgcacttgt aagaccaaca
      541 aaagagaaca ttatcattga attataccat gttgatttgt tctttataac agacttaata
      601 ccatcaacaa tattaacctt ctctgacttt tcatagggtt cagtatctac attgaatcca
      661 tactcttttg gtgtatctct aacaataata tacataaaac atccaattac aacaccaatt
      721 ataccaatta ataaaaatga atttctccaa ccaacaagtt cagacaaaaa cacaagtgga
      781 aaagtagcaa gaactccacc taaatttcct acaaatgaaa atttagctgt tgcagaagca
      841 aattctgact tattaaacca tctcccttga atttttaaaa tacaaagtaa tattactgat
      901 gttcctgccc ctacaattac acgagaaata tatgctaatt ctatactttg aatcaaacca
      961 aataaaattg accctaaact tgccattatt attccaagac tgctaatttt tcttgctcca
     1021 tatttatcaa tcaaaattcc tgcaggtatt tgcataaatg catatgcata taggcaaaag
     1081 gatgctatat ttgaaatttg tatagaatta aaacctaact catttgctaa attgtctgaa
     1141 acaacagcag ttgacatcct taaaaataat actaaaacaa atgaaaatgt aattataccc
     1201 caaacactcc acttagcctt tgtataactt ttcatatctt ctccctcaaa tccatatatt
     1261 tttaaatttt cttgatataa cttatataaa gtgtatagtt attcttacaa atttttacac
     1321 gcaatatcat tatacaacat atgtatatat ttaactagaa ataaaacact attcatatta
     1381 tatacatgaa ttaactacat aaataataag agctatcttt taacattact caaaagatag
     1441 ctccatataa aaacatttat tctaaattta attttttatc taatataaat tttgtagcga
     1501 aaaataacgc tattgcaact acaagactac caagtattgc aagcataaaa ctaggactct
     1561 gataaagatg atataccata tttgtatctt catttggtaa gatgcttcca actgcatctc
     1621 caacaattga tattactata tttatagcta taaaagaagc aaatgccact atgtttctat
     1681 gtttattgaa ttttggcaat tgtcccattg agattgatgt atataataac aaaataaatg
     1741 atgaatatga aataaacatt actactacta ataataaaat atctaaaaag ttttcagaaa
     1801 tccatttaaa actagtgtta aacaaatcta aaattccaga agtaccaaaa agcataagaa
     1861 ctccaaaagt gaatactgca actataaaac taagtacagc gtatataagt gctgttatac
     1921 atttagataa tataagtgaa ctagtgctaa caggaagagt aaacattaaa taaccttcat
     1981 cccccaaaag attttttcta aacctttgaa ttgtaactac aatagttaaa actcctaatg
     2041 ccataaaaag acttgttaat actagtattc ctattccctg tacctgaagt atatttgtat
     2101 tcataaatat tccattaaat acagctacta tcaatatggc tatatataac ggtataaata
     2161 ttcttccact tgcttttaac tcatatttta ataatttacc taacatttaa attcctccct
     2221 aaataatgca tctatagatt ttcctttttc ttctcttatt gcttcaacgt ctccttgtaa
     2281 cactatttct ccttttgata tgaatattat ctcatcacat atgttttcta tttcacttat
     2341 taagtgggta gcaatcagta aagtactatc ttcagaataa ttttttaata tagtcttaag
     2401 tatatatgac cttgcagctg ggtcaacacc acctataggt tcatccaata tatatatgct
     2461 cgcatttctt gacattacta aaataagttg aactttttct ttagttcctt tagacattgt
     2521 ctttagcttt tcatttacat caatatctaa gcttttaatc atctcatttg ctcttctaac
     2581 atcaaaatca ctataaaaat catagaaaaa cttaagtaaa tctgatacct tcatccaatc
     2641 atttaaataa gttctttctg gtaaatatga aactattttt ttagtatcta tggatggttt
     2701 cattccattt atcataattt caccattatc aggctgtaat aatccattca ttagcttaat
     2761 cattgtactt ttaccacttc cattaggtcc taacagcccg actatttttc cctttggtat
     2821 atttaaatca atatttttta aaacattctt agttccataa ctcttgttta cacctttaaa
     2881 ttcaactatt ccatcttgag aaacattgtt tatattttta tccattttat aattctcctt
     2941 tcagattttc tgtaataagt tttagcattt catctctatc atatccaagc ttttctaagg
     3001 ttacttttaa tgtatttatc tccacattag ctatttcctc tttcatagct tttatttttt
     3061 ccttatcatc tgttacaaat cttcctttag ttctctgact ataaactaaa ccttgtcttt
     3121 caagttctgt tagagccttt tgcattgtat ttggattaac ttccgcatct tctgctagtg
     3181 ctctcactgt ttctaattta gatcctattt taatttcccc tgaaattatt tttaacttta
     3241 aatgttctac taattgtata tatataggtt tgttattatc aagttcccat tccatgttca
     3301 tgcctcctta ttatattatt gtattaagtt gttaatacaa taatataata atacaaagta
     3361 tatgtcaatt actttttagt aaaagtataa aaaattttac aaaaaaaatg aacactacat
     3421 tagagtccat ttttaacaca atttattaaa ttctttatat tacagattaa ttaccctctt
     3481 accaagttag atacaattaa atgttcaaat tcttcaattg gcattggctt tccaaacaaa
     3541 tatccttgtg ccatatcaca ctttacttct tttaaaagtt taaactgttc ttctgtctca
     3601 actccttctg atatgacttt catacccaac tcttttgcca tttctattac tttagtaaca
     3661 attattttgc ttcttttact attataagtt tccataataa atttttggtc aagttttaag
     3721 atatccatag gaatttcttt taacatattt aatgatgagt accctgaacc aaaatcatcc
     3781 atagatatta aaaatcctat tttttttaaa ttattcatta tatcaattaa aatatcaaaa
     3841 ttatcaaata tgatactctc cgttaattca agttctatat actttgcagg tatattgtat
     3901 ttatcaatta tatttttgta tgtttctata aaattattgc aatataaatg tatccttgaa
     3961 acattaactg aaacagtaac tggatttatt cctgcatcaa tccattttct aaggtatata
     4021 catacttctt caagcacata taaatctaaa tttataacaa aaccgtttct ttcaaaaaat
     4081 gggataaatt catttggagg tattagccct ttatcaggac gtttccacct aacaagtgct
     4141 tctgaacctt gtatctctcc tgtattaagt tctatcttag gttgtaagta tataataaat
     4201 tctttatttt ctagacttga aaacattgta ttttccatct ctttttcttt tatctcttgg
     4261 tcatgaatct tattatcata aaatgcatat gaacttttat gtcctccctt tgccatttta
     4321 tgaggtatat tagccctatc tatgattgag ttgatatctc tatcttctgg aagtataaaa
     4381 tagattccac aacttaaaac taacttatat ttttcttctt gattattatt aaacaagtct
     4441 aattcttcaa atatagtttt taacctcttt gttatatctt cttcttctat atatttcata
     4501 attataataa aattatccgc agatattctt gcaaacattt cctcttcaaa tatattactc
     4561 ttaataatct ttgttaaatt acgcaaaatt tcattcccaa cttcatatcc aaataaatca
     4621 tttatatatt taaatttatc aatatctata tataataaag catactttac ttgagtattt
     4681 ttacatagta ttttgttagc atcaattaca aatttatcta tagtatttgc tcctgtaaga
     4741 ttatctgtat atgctatcct ttgcaattta attttaaaat ttaatttatc atatattata
     4801 tagaatataa gtattacaca tattatagcc ataataagta ttatttgtat tgcatgtttg
     4861 ctaataagct gattattaga cacaatagac tcaaagttat ttatggagtt gtttttatag
     4921 agtttgcata aagaatagac tggtgttgtg tatgtaattt gtaaaattat acaaataaat
     4981 aaaaaaaaca tcaatttttt ttgtaaattc ctcataaaaa tttcctccca ttaaaacacc
     5041 tctattttat tagcaaataa gtacatctca aaagtttcca tttcttgtaa atggatagtt
     5101 ttgaaaagaa aatattaaaa ttataatata aaaataaaat ttttcagaca atttaaacaa
     5161 aaataatcac cttttaataa ataattatat gccaaaataa tccaatttat tttctattat
     5221 acctatatga aatatataaa tcaatagtta agacaatgaa tatactacat tctataatta
     5281 tagtagcaga aaattttaca aaattaaagt agatataaac tatccatttt acaagaaatg
     5341 actacttttt attaaaatac agtatttgca acatattcac actttatttt ttcatttttt
     5401 tcttattaca atggtttcga caaatcagta attaattttt ataaatgaat aaatcaaata
     5461 attcctaaat taattttttt agtatctcac tttcaacttg attatttaaa tata
//
LOCUS       DAEMHZ010000186         5510 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000186 DAEMHZ010000000
VERSION     DAEMHZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.187"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..351
                     /locus_tag="KPK72_003539"
     CDS             100..351
                     /locus_tag="KPK72_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG2490709.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            463..1242
                     /locus_tag="KPK72_003540"
     CDS             463..1242
                     /locus_tag="KPK72_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2490710.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(1377..2027)
                     /locus_tag="KPK72_003541"
     CDS             complement(1377..2027)
                     /locus_tag="KPK72_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490711.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(2024..2527)
                     /locus_tag="KPK72_003542"
     CDS             complement(2024..2527)
                     /locus_tag="KPK72_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2490712.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(2576..3091)
                     /locus_tag="KPK72_003543"
     CDS             complement(2576..3091)
                     /locus_tag="KPK72_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2490713.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            3371..3898
                     /locus_tag="KPK72_003544"
     CDS             3371..3898
                     /locus_tag="KPK72_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2490714.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            3882..5285
                     /locus_tag="KPK72_003545"
     CDS             3882..5285
                     /locus_tag="KPK72_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2490715.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         2022 a          649 c          872 g         1967 t
ORIGIN      
        1 tagttttact ataagattat gataaaatac acaatactat gataaaatag tactaaataa
       61 tagatttaat tttgcaaagg ataaggtgtt ttaataaaaa tgaatgtaga aaacgaatta
      121 cttaaaaacc tagatagatt gcatacaaca gagttaggcg ttgtaagaat taagaaaaat
      181 ctttctttgg agacaaatga tgtagtgaac tggtgtaaaa ctaaaataga atctccaaat
      241 gccattatta atagaaaggg aaaaaactgg tatataagtg tatatgattg tattataaca
      301 gtaaacgctc acagttatac aattattaca gctcataaag aaaagaaatg aattgcaaaa
      361 tacaaaggtc aaaagcattt tccactgttt gtagtaccta tgaagaagta tataaaataa
      421 tattactttt gaataataat ttaaattttg gaggttgaaa aaatgaatga tactgtaaaa
      481 gaaaaattgg cagagtcact aacttctgat actactgata ttttacctta tttgccttat
      541 ttattacaag atttttggga attaggaagt tcaccagaac aaatgttaca attatttaga
      601 aagcatatac cttttaatga ggatacaaaa gttttggatt tagcttgtgg aaaaggtgct
      661 gtatctgtga agcttgcaaa agagttgaat atacatatca aaggtattga tttgatacct
      721 gaatttattg attatgctag aagaaaggct attgaatata aggttgatgc tttgtgtgat
      781 tttgaaatga atgatgctaa tagttctgta aaaattgagc acgattatga ttgtgtgatt
      841 tttggagctg taggggatat tttaggtaat tataaagaaa ctataagtaa gctattacct
      901 acaataaaaa gtaaggggtt cattttaatt gatgatgcat atgtagtaga taaatctaca
      961 aatgaacagc ttaattttga gagggtgtat cccacttaca atgagtggtg tatattgttc
     1021 aaagaattag gtctgaaact aattgaatgt ataccatgtt tagaacttga aaatgatgtt
     1081 gaagaggaaa tgaactggat tataactcgt gcaaatgagc taattaaaaa atatccttct
     1141 caaaaagata tgtttgaaca atatgttatt agccaaaaaa atgaatatac tgatttacag
     1201 caggaccttg ttggagcaac ttggatttta caaaaagaat aagataaata ttagactaga
     1261 taatcaatgt tatagtgaat tataccatgt ctagtgaata taaataacat aaaaaaccgt
     1321 caagatgaat agtttattcg tgcttgacgg tttttagtat ttgttgaagt aaaattttat
     1381 ttctttagta tatcactttt agtcaatcca tttgatttag cttgagtatt ccattgatga
     1441 ttccccataa gtttatatag gaatctaggg aagttaggtg aaataaaaat agtacttttg
     1501 ttggaatcat ctatatgtat attatcacta agtatatttt ttgaaagttc ttttaatgca
     1561 attcctaaat cttttttagg tcctttaccc aaaggtacgg attctagtga accaaacatc
     1621 tccccagctc caattccaat accttgaacc catgtaaagt tgattttttt acaccaatgc
     1681 tctatcattt gaattgccaa atgattatgt tcaccttcat tgaatccaca gttaactatt
     1741 gcatacactg gaataggttt tttattttgt gtttttaaaa atttttcaca tttctctaaa
     1801 aaatgaagta aatgagatgg aataccatct acataaagag gaaatgctat tattaatcta
     1861 tcacaattaa gtatggcatt aagagcgtca ggatacttag atgtgctatc tactttaaat
     1921 gtaaaaactt tattatcatt ttgaataagt tgttttaatt cagataaaaa atactgtgaa
     1981 ttatttttat ttacttttgg actaccattt atcattacta tattcatatg gattcacctc
     2041 ttatatcttg tatattttca tagaaacaaa cagagtgttc ttttatattg aaatttatag
     2101 catttccatt tactaatttc gtagcagttt ctttttcagc ttctgtaata caatctccat
     2161 agaatatagc ttttaaattt atctgtttat catatctact tttatgacgc atcatattat
     2221 cttttattac aaagtatgga agaatataat taatgctttt atcaagtacg tttttgacaa
     2281 aagggctaaa ccctccgtaa cagcatctgc ttattataat aattccatca gattcattta
     2341 taagtttacc catgttgctg taataatctt ttacaacaca ttcagaagga gttttaatcc
     2401 agcaaccaaa acaaccaata catttgtgga tttcttttgt gtttggaagt atttttatat
     2461 tgtcatcaag tttgggtaat aacttttcta attctttgtc agttaaatca tgtattaaaa
     2521 gcctcataaa gtccatctcc ttttattatg tatgtttact tctaaaaatt atatcttata
     2581 gatttttcag taaatcttta caccaattaa taacaaaatt ataatagtca actccataaa
     2641 tcattgttgc atacatatat gtatattggt caacacaatg attttcttct gcatcatttt
     2701 tttgtttact taatttattt agaagtaatt ctacttcagt tattaacatt tttaagtttg
     2761 tttcaattat ttctttgggt agatacttgt aaaagaatat attaacaagg tggtcttgct
     2821 ttgttttaga aaaatttata ggtttcttta gccattcaag aaatacttct ttaccagtat
     2881 tagttatgga atataatttt tttaatttgc taccatctat aacttcgtga tagtgtatat
     2941 atccttttgt ttctaatctt ttaagtgcag gatagatact tccaaagctg gcgtcaaaaa
     3001 agtacgaggt actttctgac attatttgtt ttaagtcgta tccacttaat tctttttcca
     3061 ttaaaaatcc taaaataatg tattctaaca tattacctcc tggtatcaat tagatatata
     3121 tcgattcgat atatttatta ttacagataa taaatcttac gtcaagatgc tttttaaaat
     3181 aagctaaaaa gaattaggag ttattgataa atatgcaaaa aaattaatat aaaaggtaat
     3241 taaatatcaa tgaagattta aaaatagtta aatcaaagta agagtctaat atataaaatt
     3301 tatagtaact ttatggttta aatataaact tgtgtgctat tgacaaagta taaaattagc
     3361 agtaaaataa ttgcatgagc aaatagatta ttgcgaacgc aaaaaaataa ggacaataga
     3421 tattaaagga gagaatatga gagagctatt aaaatgggtt tccattataa atagatacaa
     3481 tacaaattat attgacagga aattggcgga tattggaatc aataatagcc aatattttta
     3541 tgtattacac atatgtaata atccaggtat tacacaggac actatatttg agaaaattct
     3601 tgtgaatcct agtaatataa caagagcttt ggcaaattta gaaaaagaag gctttataac
     3661 aagagaacca ggtgtaaagg ataaaagaac atggcattta tatcctacag aaaaatcttt
     3721 aggatgttat gatgaaatac taaaaataac aaatgggtat gttgaggagt tgttgagttc
     3781 atttgagaaa gaagagcaag agttgtttat gtcaatgtta aagaaagttg catttttggc
     3841 tattgatatg aatgaagaag caaaattgga ggcgaaggaa aatggaagaa aaatataagg
     3901 agccattgga atatgaaaag gtttcaaagt tattaactaa gtacgcaatt cctagtatta
     3961 tagcaatgtt agtaagtgct ttatataaca ttgttgacca aatatttatt gggcaaggtg
     4021 ttggtgtatt gggtaatgct gctacaaatg tcacttttcc actaacaaca atatgtactg
     4081 caattgcatt attgttagga attggaggag cgtctaagtg ttcattggaa ttaggtgcta
     4141 aaaattcaga aaaagctaca aaagcagcag gaaattctat atgcttgatg gctgtttttg
     4201 gaataagttt atttatagta gtatcaatat ttttaacacc actattaaaa ttctttggct
     4261 ctacatcaga aatactacct tatgcacaca catatacaaa aattacatca attggattac
     4321 catttcttat aatgtccact ggaatgagta agttgatact tgcagatggg agaccgaaag
     4381 cttctatgtt ttgtatgtta ataggagcag ttattaatac aattttgaat ccattattca
     4441 tttttgtttt taatatggga attgctgggt ctgctttagc aactgttatt gggcaactta
     4501 tatcttttgg aataagtgtt cattatataa agaattttca acatattaaa ttacataaga
     4561 aaagttttaa attagatagt ataatttata aacatatatt tgcgctaggt tcaagctcat
     4621 gttttaatca attagcaatg actgtaacac agatagttat gaataataca ttggcatatt
     4681 atggggctag gtcagtttat ggaagtgaga ttccacttgc ttgtgtcggt atcattatta
     4741 aagtaaatat gatttttata tctataatta ttggtatatc acaaggtatg caaccaatta
     4801 tagggtataa ttatggggct gaaaagtata atcgtgtaaa agaagcatat aagtttgcag
     4861 tgggaagtgc aactattata tctgttattg cattcttatg tttccaattc ttcccaagac
     4921 aaattacgtc tatatttggt aacggaaatg agacttattt tatatttgca gaacattatt
     4981 ttagaacttt cttgttctta acatttataa atggtgttca aatagtatca tcaactttct
     5041 ttacatcaat tgggaaatca gtgttgggac tttttacttc tttgactcgt caaattttat
     5101 ttttaattcc attgatagta attttaccaa tgtttatggg aattgatgga gtaatgtttg
     5161 caggaccaat agcagatgca gcagcggcaa tggtatgtat atactttgca gttcatgaaa
     5221 tgaaatcatt gactataaag caaaataatt tgatgagtaa ccataataag attgcagagg
     5281 tataagacaa tatttaagaa gtatagaaaa ttaatttaaa aaatataata aattaattta
     5341 ggaagtgtag aaaattaatc taaagaagta taataaacta atttaggaag tatagaaaat
     5401 taatctaaag aagtgtaata aactaattta ggaagtatag aaaattaatt taaagaagta
     5461 taataaacta atttaagaaa tatagaaagc taatctaaag aagtataata
//
LOCUS       DAEMHZ010000187         5490 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000187 DAEMHZ010000000
VERSION     DAEMHZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.188"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(70..1377)
                     /locus_tag="KPK72_003546"
     CDS             complement(70..1377)
                     /locus_tag="KPK72_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG2490716.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(1802..3577)
                     /locus_tag="KPK72_003547"
     CDS             complement(1802..3577)
                     /locus_tag="KPK72_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490717.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(3574..3711)
                     /locus_tag="KPK72_003548"
     CDS             complement(3574..3711)
                     /locus_tag="KPK72_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490718.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(3789..4358)
                     /locus_tag="KPK72_003549"
     CDS             complement(3789..4358)
                     /locus_tag="KPK72_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2490719.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(4542..5177)
                     /locus_tag="KPK72_003550"
     CDS             complement(4542..5177)
                     /locus_tag="KPK72_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2490720.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         1698 a          994 c          527 g         2271 t
ORIGIN      
        1 gagggtacta attaaattta tttatgctat ttttgttata cctcataata caaaacatat
       61 tataaagtac tagtcaactt tattcatatc attcattaca tttaagatta tctcatctgt
      121 tatagacata cctttgtcac ttactctacc taagttttgt atactttgct caacacttga
      181 accaactata ccatttagtg gtggtacaaa acaatcttgc ttagctatta tagcactttg
      241 tatagctgca gatgctgcag aagataattt taatgcacaa ccagctttcg caccgtcaca
      301 tatcattcca cttaagtcag ctatcatatt ttctatagct ccttctattt gcttttcagt
      361 accattcata agccaagaga taccagcagt tgctccagta gaagcagcaa caccacatcc
      421 acatacagca gataatcttc ctgtatagtt cttaatataa ccagttacta gatgagatat
      481 agctaaagct tttgctagtc tttcatcaga ttgtgggaat tttttattat aagcaacgat
      541 tggaagaatt gctgttaatc catgatttcc acttccattt gaactcataa caggcatttt
      601 tacccctgcc attcttgcat ctgaagctcc agcagttaac atcattgcat aatttataac
      661 atcattacct aaaagacctt cttctataga ctttttaatt ccatacccga caccaatacc
      721 agttttttgt tttaatccat attcagccat ctcttcattc atttttactc catctagtaa
      781 aaattctata tctttaaaat caatttcttc tacatttttt attaattctt gtatagttac
      841 tgtatccatt aaactttcag ctttttctgc cgcatcattt gaagctgata cttttggttc
      901 attgtctaat aatacttctc catctttttc taagaacgta aaattatcat gtttagttct
      961 tattctcact ttagcaatat gattttttcc ttttaacaca acttctataa atactttctc
     1021 tcttgtatca gcaggtgtta ttgatagtgg agtattatcc atataatcct cagctatttt
     1081 aacttcctct tttgttagag tttctaacac acttaatcca ttttcagaat gtccacctac
     1141 tatacctaga gctgctgcaa tttttaatcc taatctttca gtacctggta tacctacgca
     1201 cataccattt ttatagatac taggactaac taacatacga ttttcaacta tttcttctcc
     1261 taataattcc tttgcctttg cacatgctag agctaaggct actggttcag tacaccctac
     1321 tgctggttta acttctgcct ttaacatttc cactaattct tttcttatat ctctcattat
     1381 atataaacct cccaattaag tttagattta gtactcaaat aattaattaa ataatttctt
     1441 atctatattt atagcaaaat acatgccata ttttttatat ctttttatat ttttagtctt
     1501 atattctata accattggta tgactgcctc tatttatgta ttttttttat ttgtatagag
     1561 tatttatatt ctttaaaatg gagaaacata tgacaatttc gcaaaaagac aaaaaaaagt
     1621 ctcaaaatga gattctatat cattttgaga cggctcaaat atattatttt ttaacttata
     1681 aaaaccattt tttaattcat aaaaaaagat tctgcaaaac acttatagta taagtttaaa
     1741 gctttcttta aagttatgaa tttagaaaaa tttaaatttt atatagcttt ttattatatt
     1801 actatgaaat tatattgtat ttttcaattt ttctatataa tgtagctctt gatattccaa
     1861 gtgctttagc tgttaattct ttatcttttt tataatcttt atacttattt aaagctttta
     1921 ttatctcaat cttctctagt tcatcaagtg gaattatcct atcgctttta acttctaact
     1981 cttctacaga ggtattctta attttttgag gaattacatt cattgtgatt attgaagaag
     2041 aagacatatt cacactatat tcaataatat tctgcaactc tcttatattc cctggccact
     2101 tatattccat catagcatca acaaccctgc tatctatgtc aataacattt ttatctaatt
     2161 tttttgaata ctctttaacc atatgcttga ttataagtgg gatatcttct tttctctctc
     2221 ttaaacttgg taattttata ggtataacat ttaatctata atataaatct tctctaaaag
     2281 tgccattttg aaccattctt tctaaatctt tatttgtagc agcaataatt cttacatcta
     2341 ttgaaacatt tgatttactt ccaattttat taagttcctt ttcttgtaat actctcaata
     2401 actttgactg taaatgcaaa ctcatatctc ctatttcatc taaaaatata cttcccttat
     2461 gagctacttc aaacattcca agctttccac cttttttagc tccagtaaaa gctccctctt
     2521 catatccaaa taattcactc tctaataaat tgtctggtat agcagcacag tttacagcta
     2581 taaaaggatt atctactcta ttgctatggt tgtgtatggc tcttgcaaat agctccttac
     2641 ccgttccact ttccccagta ataagtactg ttgatgttga tttagaagct attagggctt
     2701 cctttttaga atttcttata gctacacttt ctccaattat gttttccaat tttattctat
     2761 aatctttatt catcttattg tagtttttta tagcatctct cttatctata aaatcaataa
     2821 catatccctt taactcattt tttaatacta ttttattgat gttgtatatt cccttcgaat
     2881 catatccgta tcttttataa aaaaaactac aggatttgtc cttcttaaag ttatttatag
     2941 aaggtttctt tataaaatct aattcgacaa atatgttttt gccagttata ctatctttta
     3001 tattgaaaag ctctttaaat tttgaattat atttatcaat ttttccatcc acatcaatag
     3061 acacaacagc tttgtccata ttgtttaata gtgtctctaa ttttttcttt tctaattcaa
     3121 gctcataagt tttaatttgt gcttttaatt tattagatat taggtctgcc attttgccta
     3181 aaaagtttat aagaccactt ttattatttt ctataatttg tgcttgttct tttgtaaagg
     3241 ctataaggcc tataacacca tgagaatcgc catcacatat aataggacaa cacacctctg
     3301 caaactctac acaagcccca cttttatagc attccctaca tatatcacta ctctttgggt
     3361 catcaattag tattgtaatc tgttctgtaa gtgatttttt aaaagccgta tatttatcta
     3421 ctttctcacc tattttgtct atatatactc ctgtacctgc aatcctaaat aactccttat
     3481 ttactatagt tacatctata ttgagtactg atactatagc ttcagctata ttttgaatat
     3541 cagattttat atattctaaa cctatcataa tcatcattcc ttatttttct ttaacataag
     3601 tatttgtctt atttctttaa gaatcaagta tatcttattt aaagtaaatt ggattgacat
     3661 caggattaca aagagcataa caattattat taactcataa tttatttcca tctaaacatc
     3721 tccttataac aaaagactag gcttaaccta gtcttttata ttatatccat atttatattt
     3781 taactcattt atcttctttt tcccacataa atatatttta atttatttga ctctttaatc
     3841 ataccatcaa agtttaatct tgaaaatata ttaaatactt catgtggata gtgaatcttt
     3901 gcaacagaac cacaaaattt atgtttaaga tagtttaact taacttttaa attctttaaa
     3961 tttaaatcct ctaatataac ctctccattg ccctttaaca ctctttgtat ttcagatgaa
     4021 gctttttcac aatctgtaat aacattaaat tggtcaacta ttattatctt gttgaactga
     4081 ttatcatcaa atggtagaaa ctctaactta cctttaacta cattgcaatt ttcattatac
     4141 actagagaat aacagacatc ttcataatta ctattctcca gtatagttac agaagttcca
     4201 agacctctca atctcttgcc taggtttcct atatgaccaa caagcaatat tttatcacct
     4261 acattatact caattgtatc caaaaaacat tctaattcat cagatgtata tttagcttta
     4321 accggctgtt tatttatctg acataatatg cttttcacaa tgcttcacct cttcatttcc
     4381 tcaattataa attatgtaca gattacatta taataaatgg cgagcaacta ttcaagagat
     4441 atttcattag tattttttta aacaatcttt atacatttta atatgatatt ataccaaatt
     4501 tattaggatt ttattcataa aaatcagtct atttttcatg tttattcatt gttattttca
     4561 tccaattttt ttattttcga tatggatact tcatatgcca tttttttgac cacattattt
     4621 tcatctattt ttttctcata ttctctactt tgaaccctac cccaaagctg tattctatca
     4681 ccaaccttta gattctttgc aaatttagca tttctacccc aaactattga tggtatataa
     4741 tcagatttat tataaggtct gtttatagca actaataaat cggttatttc ccttcctaaa
     4801 ggtgtcttcc tatacacagg ttccttacat acatatccat ctaaaaatat actattaggg
     4861 tctttatttt cctcgtctat gctttttatt tctctgacaa acactgtaag cactaatcta
     4921 tttttattat ttaatgtttt gttatatgat cttaattggc ctacgacatt gactaaattt
     4981 tgtttatcaa ggtcaatatc ttggagaagt ctttctgata cagtcattgg aagtatatca
     5041 aaagaatcgc ttagtctgtt tatttttatt ttcatattat aaaatttttc tccaaatatc
     5101 tcatggctaa attctagctt attgtctaac tcccctctta agtttactac gttattctca
     5161 tctttaacag ctatcatatt ttatccctcc acaattgtta attcttatat ttctggtatc
     5221 agcttatata ataaatatat ttataaggga tgtattttat tacaaatctt tgattgctgt
     5281 ttaaaagttt ttatttagtt ataaatttca tttaatattt cacttttata attataaaat
     5341 tattatcttt ttgtaccaca tatactaatt aaactaccta tttcagtttt gtattaatta
     5401 taaaaatcct attatataat taataagtca tatataactt aaatttattc actctatata
     5461 agcaacttca acgaattaaa tttattcaaa
//
LOCUS       DAEMHZ010000188         5477 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000188 DAEMHZ010000000
VERSION     DAEMHZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.189"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..617
                     /locus_tag="KPK72_003551"
     CDS             117..617
                     /locus_tag="KPK72_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG2490721.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            674..1660
                     /locus_tag="KPK72_003552"
     CDS             674..1660
                     /locus_tag="KPK72_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2490722.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1710..2432
                     /locus_tag="KPK72_003553"
     CDS             1710..2432
                     /locus_tag="KPK72_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG2490723.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2461..4395
                     /locus_tag="KPK72_003554"
     CDS             2461..4395
                     /locus_tag="KPK72_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2490724.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4388..>5477
                     /locus_tag="KPK72_003555"
     CDS             4388..>5477
                     /locus_tag="KPK72_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG2490725.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAY"
BASE COUNT         2286 a          417 c          943 g         1831 t
ORIGIN      
        1 tagttcgcta aaataatttg tttttataaa attactgttt aagtttatag attttgtata
       61 aaataaagtt atgttattaa tttttttaaa aataaaactt ttactaggag acagatatga
      121 gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat gcagttttaa
      181 cattatcatt gggatttata agttatggac caatacaatt tagggtagca gagattatga
      241 tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt tgttttttag
      301 caaatgtaat aggaccatat ggtgtacctg atataatttt tggaacatta gccacattta
      361 taagtgcaag tatgatttat attactagaa agttaggtaa acaaaataaa agtactttag
      421 ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta atgttaaatg
      481 tattctttgg gcttccatta attctatcta tgatacaggt aggttttgga gaatttgtag
      541 tagtaacaat tgtaggagta ccatttttct gctttttaat gaaaaaatat aaaaatgtca
      601 ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat tattagtgaa
      661 aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat atattaatga
      721 taagcaaaag tttacaataa aagagttgtc agaggaattt aatctttcag ctaaaacaat
      781 acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag aacaaggtag
      841 aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat ttacagaaaa
      901 agaagttatg attatactat ttgcacttaa gtcacttcag atttataatt ataagttaat
      961 ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa gcagtataaa
     1021 agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata ctaatgatgg
     1081 agattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat ctctaatttt
     1141 aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc ctataggtat
     1201 atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg ataaaataat
     1261 attagttcat atagataata taattgatat aaaaagaaca ggagaaagta aaaaaataca
     1321 tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa atagttcaac
     1381 tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag aaggtgtttc
     1441 taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg gtagtggaat
     1501 aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt tattttttat
     1561 tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt atgataaggc
     1621 caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa tgttgaaatt
     1681 aaataattat taaggagata tacgttataa tggaaaattt taataagaat gctaaaataa
     1741 ttggtattgg agctgaaggt attaatataa taaatgaagt agaagaaaaa ataaaagcaa
     1801 atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat gttagaagtc
     1861 ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa catattagag
     1921 atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct ataggaatgg
     1981 attgttcaga gaaggaaaat aaagaagatt tagagcttgg gagagagttt aaaatcaata
     2041 atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct atttctgatt
     2101 cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga gataaaggaa
     2161 ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata gctgacattt
     2221 tatttgacag gatggaatat attggtgagg aatttataag taagaaagga attgtatttg
     2281 ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt aatattcaga
     2341 gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa aatttaaatg
     2401 gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag gagagtgtat
     2461 atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt ttatcaggaa
     2521 acagacacac cacagatttt tagagaagaa agcataattg cacttggatt tgatataaaa
     2581 ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat taatgagaat
     2641 tgtaatgata aagattttat aaaaaagttt atgtcgatta aaaaaggaga ttatttttta
     2701 tataaagcat atcaaaatat agaagaaaaa gttaaatgta atgtgtctat tggcatagtt
     2761 gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac actaccagta
     2821 aaatggatgc ataattttga tacagtttca aattgtaaag tatatacaag tgaattttat
     2881 aaattaaata ataatgatat tagttcatat ttagaaatta ttaagtcaca agataagaaa
     2941 ttattgagaa aaagaataga agaaagtgaa aataataaat ggtatttaaa aaactattat
     3001 tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc cattaatcta
     3061 gaacaattag aaagcctaaa aaatataaag acagatgatt atattttatt gtataatagc
     3121 gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt atgtcaagtt
     3181 atggaagggt tcgataaaga gaaagtaagt gctttggagg gtggatattt tttccctgtt
     3241 agatggatag aatatgaaaa ggagaatata gacggaaatg catgggttat atttgaaaat
     3301 aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatgccatt aaatactata
     3361 ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt ttacaatcta
     3421 gtaagtgaaa ctagtctaag aaacaaatac tatacaggtt acttagatta taaaaacaat
     3481 gtagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga gggtcttaag
     3541 tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat atctatagaa
     3601 gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga aaaagatagc
     3661 ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa tatagaatat
     3721 agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga tgaaattaat
     3781 agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga tgataaaaga
     3841 cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa attttctcta
     3901 cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat agcactttta
     3961 gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta tgatttattg
     4021 tctattgtag atggtataga tattagaaaa atgctatata caatcaataa aaggatagag
     4081 tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt agaaaatatt
     4141 aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga gtatttttat
     4201 tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga aaaagataaa
     4261 tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa taatgcctca
     4321 ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga ggagctaaaa
     4381 aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa gggtaggtaa
     4441 tggtgaaaat gagcttacag aaattgaata tgaaaaattg cttaagtatc tagagaacaa
     4501 taatgatgta ttaaaaagta atataataga tattaaatac aaaaaactta gatttataaa
     4561 ctatgtaggc ataatatgtt ttgaaaatgt aatacttgaa atacttccta aattgtcatt
     4621 aagtgataat cttgtaaaag atagagagat actattacag atgctttcta tatgtaataa
     4681 aattccaatt acaatgaatg aaaaaataag attgagtttg aaaaattata atctcttaaa
     4741 tttttttgtt atgtatttca ttgagagtat gcaaacacag atgaaaaaag gtatttattt
     4801 tgaatatata aataaaatag agaacttaaa tgttatgagg ggtaaaatat tattaagtac
     4861 atatgcaaaa gaaaaaggca tttctccaat gaaaataaga tgtgaatatg atgagtatag
     4921 tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt atatctattt tatgtagaat
     4981 aaatgacaat tcaattcaag gcaagattaa aaaaatttta agctattttc aaaatgtaga
     5041 tttaatttat atagatagaa aaaaactgtt ggattataaa ttttataaaa acaatgatag
     5101 atttaaagat tgttatttac tagctagact tctactttta aatttgtcta tggataatag
     5161 tcaaaataat caagaggcat tttctatatt atttgaaata aatactttgt atgaagaata
     5221 tataggaatt ttaatcaaaa gtatatggga caattcattt agagaaacat atatacaaga
     5281 taagagtaaa tttcttttga aaaatgaaca aacaggtaag aaaaacttta atttaagacc
     5341 ggatatagtt ttatatgact taaaaaatga atatgaaatt ataattgaca ctaaatggaa
     5401 agctattgaa gtagattcaa atgtttttta taggtcaagt gatatatacc agatgtatgc
     5461 atatataaca gcatatg
//
LOCUS       DAEMHZ010000189         5429 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000189 DAEMHZ010000000
VERSION     DAEMHZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.190"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(587..1138)
                     /locus_tag="KPK72_003556"
     CDS             complement(587..1138)
                     /locus_tag="KPK72_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490726.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1329..2291
                     /locus_tag="KPK72_003557"
     CDS             1329..2291
                     /locus_tag="KPK72_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG2490727.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2585..4429
                     /locus_tag="KPK72_003558"
     CDS             2585..4429
                     /locus_tag="KPK72_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2490728.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4751..5335
                     /locus_tag="KPK72_003559"
     CDS             4751..5335
                     /locus_tag="KPK72_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2490729.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2119 a          542 c          862 g         1906 t
ORIGIN      
        1 tcattaccat tatgttttaa tatactattc aattatttaa cctcttaaat tatatttaga
       61 tataggagtc ttatgataaa agtaagctta tgtgttattt gtagtaaata taaagtgcat
      121 atagtgataa aacaaaagtt tcatataaat gaagaaaaaa atataaaata atttatttaa
      181 ctacttaaaa ataaactaaa agggataata aaatagcttt taggttatgg taataaaggt
      241 atttaaaaag agtgcataag cactccaatt gtgagaaata catacacaaa tcattattac
      301 aacaatgaaa aatgtacata taagagacgt tttgtatttg ttttaagtga taatatttta
      361 atactaacta aataattatc acaatagtaa aaaagaaaaa ggagtatttt gtactccatt
      421 tgtcaaaaat ataaaacttt tatataagct tattataaca taattgataa aagtgtaaag
      481 atatataatg aatttataaa gaaaaataaa ataaaaaaga taaatcctaa tcaaaggatt
      541 tatctttttg tatagaaaat aattattcta ttattaaatg gcatggctaa actggataat
      601 aatttattat tactgctttc aatgtttttt ctccttgatt atggtatgta tgaattgatt
      661 cagctgaaaa acaaatagag tcattttctc taagttgata aatagagtca tttacttcta
      721 attttaattg accttctata atcataatat attcctgtga tttttcagaa tgaccaacag
      781 atgtataact atgaccttct tctaattcca tttgaaataa ttcgaagttt ctagttggtg
      841 tattgggata ataacaataa agacgataat ctttattttc tgaaacttgt acatctatat
      901 cagtttttga tacaataaaa gtttcatttt gaggttgttc cagtattgca gtatatggaa
      961 catttagtcc acttgcaatt ttccaaattg tgtttactgt tgggtttgag tctccctttt
     1021 caatttgcga aagcattact ttacttacgc cagataattc agcaagttga cctagactta
     1081 agttacgttc attacgtaat ctatttaaat ttttagcaat aacagtatta atactcatag
     1141 tagctcctta aaatgtaaat tattgacaaa tataatttac gaatgtataa tttatttaac
     1201 attatttata atttacaaat gtataatata atgaacgtta aatacactgt acatccgtaa
     1261 tataaattat actgcataaa gagttgtttt tcaacggatg atagttgaga gattaagggg
     1321 gaattgtaat gcgtgtactt aataatgtta cagaggctat aggcaatact catatgttac
     1381 atttatccaa aatagcagat gaatttggag ttcaaggtaa tatttatgca aagatggaat
     1441 acttaaatcc gggatttagt aaaaaggacc gtgtagcatt acaaatgatt accgaagcag
     1501 aaaatgcagg tttattagtt ccaggtcaag cagttgtgga attaacaagt ggtaatactg
     1561 gaacaggact agcaatagct tgtgcatgta aaggatataa atttatagcg tgtatgtcaa
     1621 aaggaaattc tatggaaaga gcaagaatga tgaaagcttt aggtgctgaa gtagttcttg
     1681 tagaccaaat gcctaattca gttcatggac aggtatctgg tgaagatttg gcacttgtag
     1741 aaattgaggc tcaaaaaata gcaaaagaaa gaaatgcatt tagagcagac caatttaatt
     1801 tagaagcttg taatcatgca catgaaaagt atacagcaga agaaatgtgg gaacaattgg
     1861 atggaaatat tgatgtattt gtagattttc ttggaagtgg tagtactttt gcaggatgtt
     1921 caaaaacact taaaaaacat aattcttcta tcaaatgtta tgttgttgaa cctgaaacag
     1981 cagcaattta ttcaggtaaa gaaataactg accaagggca taaaatacaa ggtggaggat
     2041 attctatgga tttgccatta gtagacaaga aacttattga tggttatatt caagttacag
     2101 acgaggaggc aactgatgta gcaagaaaat tagctaaatt agaaggtgta tttgcaggat
     2161 tttcttcagg ggcaaatgta tgtgcagcaa ttaagttgtt aaagagcagt gaaaaaggaa
     2221 aaaatatagt tttaactctt aatgatagtg gtttaaaata tttaagtact gatttatatg
     2281 aagaattgta atatagcaca atagttatgc aatgtttttt aaattaagta tatagattta
     2341 aattataaaa agtatctcaa tgtaattttt gaaagttata aaggagatac ttttttattt
     2401 tgtaaatatg gagtatccta cttataagta ttaagctata tagatattga tgattagcta
     2461 tgcaataaaa aagagtatgt gtttttgcat ttatacatca taacttggaa taatacatat
     2521 taatgttcta aatgtattta aaacatatga taaaattaga acaaatagat acagggagat
     2581 aaaaatgcaa aagagaattt taagaaattt tatatttacg ggtaaaaata ttttaagtaa
     2641 acttgttttt ataataaaat ataattttag tacattaatt ttatttgaat tatttcataa
     2701 aggattagcc ttatttttta tatggccatc tattagatat atatttgata ttttaataaa
     2761 aagtttacat atagtctacc taaatatgga taattttata aaagttttga ccaatcccat
     2821 gtcaatagtt ctgataatac tatcagttat aatactagct ttttatgcat tttttgaatt
     2881 tacctctgta attatttgtt ttaataaatc gataaaatat gaaaaaatag ggttatttga
     2941 actctttaaa atatcatttg agaactctat aaaattgtta tatcctaaaa atatactatt
     3001 atttatattt gtgatattaa taattccatt agtaaataca gttttaattt cagggtttat
     3061 aggaaaaata caaataccag agtacatatt agattatata aagtctgata taattttaaa
     3121 tataatctat atgatttttc ttttaataat atatatttca gttattagat ggatttttag
     3181 cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag aaagtgttaa
     3241 tcttataaag ggaaagatta taagaacaat tatttattca ttaatattat ctttagtagt
     3301 agctattatt ggatatgcga tttatcatag tggaattata tttataggca tatggactaa
     3361 atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta ttgcatttga
     3421 agaatatttt agattgattt tatcaatatt catttttata ttggacattg gatttatctc
     3481 agcaacttat tatcaatata aaggagtaaa tatttctaaa aataatatta aaaaagagaa
     3541 aaaatctata tcaaaaattg tttttaattt agtattaata ttattgattt cttatataga
     3601 gtcagttgca ttttcttcac atataaatgg tatgtttaat acgtcatttt cattttatac
     3661 aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt tggcagcaat
     3721 agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag aaactaaaga
     3781 tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag tagataaaaa
     3841 tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct tttattatta
     3901 tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac tcaaagaaat
     3961 gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta atggacatga
     4021 aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt ttgaaaatca
     4081 atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac tggatgagaa
     4141 tttattgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt taaattatgt
     4201 tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa agattcataa
     4261 aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg agaaaatgct
     4321 tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga gtgcaattta
     4381 ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa tattctataa
     4441 ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt taacataaat
     4501 aaggttgtta aattaaggtt attcttataa aaattgtgct tatcagctat aaacaaggaa
     4561 taaaagtgaa aaaattgctc tgagattatt acagttaaaa gagttttaag atgacagaga
     4621 gatagattgt caactgtata tttaataaat cttgttattt attaaatttt atataagtaa
     4681 aatatataaa ttaataaatc ttatgctaga atatatatga aaaacaatat aaatttggag
     4741 gattaaaaac atgacagagc gagaaaaaat gttggcagga gaactttacg attgtggtga
     4801 tattgagttg ttaactcaat ggcataaagc aaaaggccta gtaagagatt ataaccaaac
     4861 agattcctcg gatttggaaa aaaaagaaaa aatattaaat gagttacttg gtggtaaggg
     4921 taaaaactta tggattactg caccgtatta tgtagattat ggaaataata tttattttgg
     4981 aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa ttatcattgg
     5041 ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc caactaatgc
     5101 tggtgataga tttggtaatg ccaaagaaga tggttcattt gaattttgta aaactcaaac
     5161 agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta tcatgccagg
     5221 tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa aagatattcc
     5281 aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaata aataaagtta
     5341 aattttcttg caaaatctca aaaaacacct ttcttacttt atacataggg tattttctaa
     5401 tactttcaca tccatattca tttgttaaa
//
LOCUS       DAEMHZ010000190         5399 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000190 DAEMHZ010000000
VERSION     DAEMHZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.191"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..1204
                     /locus_tag="KPK72_003560"
     CDS             23..1204
                     /locus_tag="KPK72_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2490730.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1346..2263)
                     /locus_tag="KPK72_003561"
     CDS             complement(1346..2263)
                     /locus_tag="KPK72_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490731.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGKVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2516..3310
                     /locus_tag="KPK72_003562"
     CDS             2516..3310
                     /locus_tag="KPK72_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490732.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIELARKEDI"
     gene            3311..5371
                     /locus_tag="KPK72_003563"
     CDS             3311..5371
                     /locus_tag="KPK72_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2490733.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2057 a          620 c          900 g         1822 t
ORIGIN      
        1 ttaaaagggg gagaaattta agatgaacaa aaaatatatt aaagcaggga atttaattga
       61 tggaaccgga aatagtatta tgcaaaataa gggtataatt attgatggag atactatagt
      121 tgaaattgaa gatataagag atgatttaaa tggatatgat gtgtacgatt acttagataa
      181 aactgttatg ccaggcatta tgaattgtca tgttcacttg acaatggagc cagttggaaa
      241 tccaaagata tattatgata atgtatcaga tgtagagctt gtagtaaata cgataaaaca
      301 acttgatgct tatttggatt caggagtaac atatattaga agcttaggtt gtcccaaata
      361 tgtggatgta caacttaaaa atcttataga caaagggtct atagatggtc ctggtatagt
      421 tacttcaggt ccagttatat gtatgacagg agggcatgga tattattttg gtattgaaag
      481 tgatggagtg gatgaatgta gaaaatcagc cagaacagtt ttaaaaaatg gagtagattg
      541 tgtaaagata atggcaactg gaggtgtaac aactgatgga gttgagccag ggtcaccaca
      601 attaactttg gaggaaatga aggctgctgt agatgaagct ataaaagcag gtaaaataac
      661 tgccacacat gcacaaggaa gaactggtat aaaaaatgca gtgcttgcag gaattacttc
      721 tatagaacat ggtgtttatt tagatgatga aataattgat ttgatgcttg aaagaggaac
      781 ttacttagtt ccaactgtgg ctgcaccata ttttattcta aatgcaggaa aagaaagtgg
      841 aataactgaa gctactctta gaaagtgtga gattgttgcc attccacaca aagagtcatt
      901 cctaaaagca tataaaaaag gaattaaaat tgcagttggt tctgatgcag gaacttctta
      961 taatgaacat ggaaaaacat attatgaaat gaaactaatg tcagattatg gtatggataa
     1021 tatggatata atagttgctg ctacaaaaac agcatctgaa cttttaagaa ttgataaaaa
     1081 ttatggaact ctagaaaaag gaaagaaagc agatgttata gttgtaaatg gaaatccagt
     1141 agagaatata gacgtacttg cagatgtgtt agcagtattt aaacttggta aaaaagttag
     1201 ataaatagta taaaaataaa aaagagtgtc taaagaaatt tttaaataat tatagacact
     1261 cttttttatt agtaataatt aaatctttta tactttagaa aatagattct attttatatt
     1321 tagatataac tctttaaaat cattcttatg caatataatc tatagaatct acacatacaa
     1381 agtatattct tgaatcatta gaatttccaa gtactataac atcatttttc ttacctagaa
     1441 cttcaacacc agttagaacc aaactaccag ctactacagt aacttgatta tttatatttg
     1501 acatataaag attatctagg atacctttat tacaacagca atcatcacaa tcgcagttac
     1561 aattacattc tttgtaatgt tttttatcaa gatagtaacc taaagatgct atgaaatctt
     1621 cataatctgc aggtgtaata tcaaatgcaa cagctttaag attacacaat gatgctaaat
     1681 ctacgtttgc taatggagta gatgcagcaa gtataaaatc aattatagca gcaagtattg
     1741 catctaatat agccccaagg tctatcgtag gaggaataac tgcatcaata agtgcaatta
     1801 attcagctac tcctggtata gttcctatag tattaattaa tccctcaaga gttaaaggga
     1861 ctggaatagg ataagatact ttaccagcta tatctattaa atcacagtta caagcagaaa
     1921 atctttcaaa agtaccatca agtccagata aattatcttt tggaggagct gaaagatcta
     1981 tacctaccaa attagcacct actataaaga aatctgagat aaaagcaaat tgattaaagt
     2041 ttacaaaagg tcttagagca tcatatctta gaagttctaa ggcctctctc atactttttt
     2101 tacaacaaca ttgttttatt ttaggttctt tatatttatt tggataatta tagtatctat
     2161 cagctacttg ataattttca tagtatcttt catttgtatt tggataatct tcctcatatt
     2221 cttgtgtaaa tctaaagtcc tctctacatt tattattttc cacgaaatac actcctttta
     2281 tattttaatt taaatgtata gataattaag cttatctata tagtaatatt atatttataa
     2341 tgtaaaaaaa tgttacaaaa gatattataa acgaataaaa aataataatt tgtcagataa
     2401 acttgtaaat ttttatgaat aatccattct aaaacaaaaa actgtgtata aaaaaggttt
     2461 atatgttata tttatataaa tataacatat gaacgagaaa ggagtggttt gataaatgat
     2521 tgaggtagat aaattgtgta agtcttttac aagggttgta aaagatgata agaataaaag
     2581 ttcaataaga aagctaaaga aagtaaaaac taaaaaagaa gaattttttg ctgtaaataa
     2641 tgtttcattt aaagttatgg aaggtgaaat tgttggaata ttaggtccaa atggtgcagg
     2701 aaagactaca ttacttagaa tgttaggtgg catattaaca ccaacatcag gaagtataaa
     2761 tatttctgga tatgattatt caatagatag aaattcagca aaaaaagaaa taggttatct
     2821 ctctggaaat acaaaactat atggaaggct ttctccgaga gaacttctaa caacttttgc
     2881 aagtctatat gagatgtcaa aagaagatat agaagaatca attgaaaata ttgtaaaaat
     2941 aatggatatg agtgaattta tagataatag aattgaaaat ctatcaactg gtcagactca
     3001 aagaacatct atagctcgtt gtttaataca ttctccaaaa gtatatattt ttgatgagcc
     3061 aacactaggt ctggatgttt taagtagtgc atctattata gattttatga agaatgaaaa
     3121 actaagggga aaaactgttc tttactcaac tcattatatg gaagaagcag aaactttatg
     3181 tgacaagatt tttatgattc acaatgggaa aataattgca tcaggaacac cacagagttt
     3241 aaaagaggaa tctggagtaa ataatttgag agatgtattc atagaacttg ccagaaagga
     3301 ggacatttaa atgagaagta agattgtaaa atacatattt aaaaaagaaa tgttagacat
     3361 attgagagat aaaaagactt tatttatgat gattgttgtg ccacttcttt tatatccatt
     3421 acttatgtta ttaatgattc aagtaatgaa tatgtctgca agtagtatga atagtaagga
     3481 cattaatata gcttttaata ataaacctaa taaggtttta gtatccatga ttgaagatga
     3541 taatttagaa aaagatacta aaaatacttc aaatgataaa ggcaaaataa gagtgttaga
     3601 tgtagataac tataaaaagt cattagaaga aaataaaatt gatgcttata taaagatgga
     3661 agaaaaaaaa tcatctataa attatcaaat atatataaat tcttctcaag aaaattcttc
     3721 taatgcacta aaaagaattg aaactgtttt ggataaatat aaaagattta ccatagaaaa
     3781 aactttatct gaaaaaggat tagatgtaca agctacttta gaaccaataa catatagcac
     3841 tgtagatgtt gcaaagacgg aagaagtggc aggatacttt ttaggtcaga tacttccatt
     3901 tatattaata ataggagttt tactaggttc aatatatcca gcaatagatg ttatggctgg
     3961 agaaaaggaa agaggtacat tagaaacatt atttacactc ccaatatcaa acttagaact
     4021 ggttatggga aaatatatgg cagtttctct tagtgcaata gtaacagcaa ttttaaacat
     4081 tttatctatg ggactgacta tggcatttat tttagttagt ggaggtatta gtagccaatt
     4141 tggctttgga aactttaatt atggagactt agtcttacca attataacta ctttaatttg
     4201 catatgtctt ttttcaatgg tagtttcagc aataagtatg tgtgtatgtt cattagcaaa
     4261 aagttttaaa gatgctcaaa actacataac acctcttatg ttaattatta tgataccatc
     4321 atatgtttct atgataccaa atatagtatt agatagagta acagctgtaa tacctgtagt
     4381 aaatatatct ttacttataa aaagtgtatt atcttttaaa agcgatttaa atttaatagc
     4441 cttagtttta gcatcaaatg tagcctttgt tatattggca atcatactac tgtctaagat
     4501 gttcaattct gaggaaatac tttttggtaa tagtaagagt ttttctttct tagagaaaag
     4561 aagtaatata aaaaaagggt ctataccaac tgtaagtgat ggtgttattt tatatgcagt
     4621 aggattagtt ctattgattt atgttggttc tttagttcaa attaaatttg gtatggcagg
     4681 tattataatg acacaattca tgataatttc tttaccgtta ttatttgcat actatattaa
     4741 agctgatttt aaagaggtat ttagcttgac agttcctaaa ttaaagcatg tgtttgggag
     4801 cattgttctt tggattggtg gatacatact tattatgatt ataacacagc taattttatt
     4861 cttatttcct caaaatatgg aagttgctga aagtttaaat aaggctcttt ttatcaaaga
     4921 tagtgtttta ctgaatttac tagcaatagc tttattacca gctatatgtg aagaaatgtt
     4981 ttttagggga tttatatttt cttcatttag taaatcaaaa gataaaaata agtctatcaa
     5041 gctagctata atatgcagtg gggtactatt tggtattatg catatggatt ttattagaat
     5101 aattccaaca tcaatattag gtattatatt tgcatatagt gtgtataaat ctggttcaat
     5161 atttgtatca atgttattgc attttttaaa taatagtgta gcagttgtgt taaatcatta
     5221 tacaactgga aatataacca acttatataa atttgttgaa gttgattttt cgaacttaaa
     5281 tgtacttaaa tttgccttaa ttcttctaat aagtcttata ttaattatgt taggattaag
     5341 agttttagat agaaagtctt taagaaatta agtataaagt tattatatag attaaaata
//
LOCUS       DAEMHZ010000191         5395 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000191 DAEMHZ010000000
VERSION     DAEMHZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.192"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1640)
                     /locus_tag="KPK72_003564"
     CDS             complement(<1..1640)
                     /locus_tag="KPK72_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490734.1"
                     /translation="MQTEWNFDYIGAGKKVTLKPGKYKLECWGASGGGRFDEWTECAK
                     GGYSKGELTLKKETILYVYAGESGYKKFSNISDWAGFNGGGRGPNEGVDPKFTTCGGG
                     ATDIRLIGGVWNDEQGLLSRIIVAGGGGSIGTSSFYSIGLGGGFAGGMGVGAGTTCTG
                     GTQYEGGVTVNSGNGSFGKGGIGNVYAGGGGWYGGAGASSSGVGGGGSGYVLTKDSYK
                     PKGYIPTSEYWLENVNSIAGDNTSNAHGYAKITLLQALPFLNISSYNSTTATFKADHT
                     DPTLLTKIEYFIDDVLKETITTDLTTEKTINYELTDNAIHTLKIVATDAEGATAEKIL
                     SISKEIMPLQSDATLQDISTKLIEIGEGFKNGKTSIINTLALKNIEASLNNTLVELSE
                     KIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKYASGTIDVVKNSSLMANLYGESF
                     GRQPGTWLKIDNLGFIPNIFVAECQYVTSNNYFFKHIVIATCNINWLWEKKDFSARIV
                     FTKEKNSNQDFSGSGIIYSNNERDVYINNKGINVPASSP"
     gene            complement(1658..2461)
                     /locus_tag="KPK72_003565"
     CDS             complement(1658..2461)
                     /locus_tag="KPK72_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2490735.1"
                     /translation="MATEKSYYTILTDIGKAKIANASLVGGKVDFVKIQLGDGGGNEY
                     NPTEEQTILKNVVWEGKVGNVKTDESMTNCLILESLIPASAGGFVVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETTKTEINNKIGDTTLLETTDKTNIVSALNEVKTSVDSI
                     ETTAEKTSYNNATSNLTATNVQGAIDEVVAKIKKFNEVNINTINDMLPI"
     gene            complement(2477..3094)
                     /locus_tag="KPK72_003566"
     CDS             complement(2477..3094)
                     /locus_tag="KPK72_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG2490736.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNAENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGATTITGHEITIYPWSPSDIETQGEINILSGNGFGYQEITIY"
     gene            complement(3087..4136)
                     /locus_tag="KPK72_003567"
     CDS             complement(3087..4136)
                     /locus_tag="KPK72_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2490737.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAAELTET
                     YIAMDEMLDQTFVDTASYYYLEKRCKERGITPLPATHTIAKGVFNIDIPLDSRFNLGE
                     YNYIATERISEGIYKMKCETTGPIFELGQLIPIEYVDKLETAELTEILINGEDEESEN
                     SLRQRYYNSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKTIAEEAVDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(4137..4556)
                     /locus_tag="KPK72_003568"
     CDS             complement(4137..4556)
                     /locus_tag="KPK72_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2490738.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(4561..4821)
                     /locus_tag="KPK72_003569"
     CDS             complement(4561..4821)
                     /locus_tag="KPK72_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG2490739.1"
                     /translation="MSQELLQVIKKAAMDAVETSNPMTLAFGTIESVNPLVVKIEQKA
                     SFEEFFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(4835..5269)
                     /locus_tag="KPK72_003570"
                     /pseudo
     CDS             complement(4835..5269)
                     /locus_tag="KPK72_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         1573 a         1093 c          627 g         2102 t
ORIGIN      
        1 ggactacttg caggaacatt aatgccttta ttatttatat atacatcacg ctcattattt
       61 gaataaataa ttccacttcc actaaaatct tgattagaat ttttttcttt agtaaataca
      121 attctagccg aaaaatcttt tttttcccaa agccaattta tattacaagt agcaataaca
      181 atatgtttaa aaaaataatt attagaagta acatattgac attcagcaac aaaaatatta
      241 ggaataaaac ctaaattatc aatcttaagc caagtaccag gttgtctacc aaaggactct
      301 ccatataaat tagccattaa agaactattt ttaacaacat caatagtacc actggcatat
      361 ttatacttag aatttaactg tgatatagta ttgttagctt gtgttaactg attcatcaaa
      421 tcttgtacac tagcgtctga actatcaaaa cttgttttta ttttctctga taactcaaca
      481 agtgtgttat tcaaacttgc ttctatattc tttaatgcta aagtgtttat aatacttgtt
      541 ttaccattct taaatccttc tccaatctct atcaacttag ttgatatatc ttgtaaagtt
      601 gcatcagatt gaagtggcat tatttcttta cttatactta aaatcttctc agcagttgca
      661 ccctctgcgt ctgtagctac tattttaagt gtgtgtattg cattgtctgt aagttcatag
      721 tttattgttt tttctgttgt taaatctgtt gtgatagttt cttttaatac atcatctata
      781 aaatattcta tcttagtcaa taaagtgggg tctgtgtggt cagctttaaa ggttgctgta
      841 gttgagttat atgaagatat gttcaagaat ggtaatgctt gtagtaatgt tattttagca
      901 taaccatgag cattactagt attatcacct gcgatactat taacattttc taaccaatat
      961 tcagatgtag gtatatatcc ttttggttta taactatctt tagttaaaac atatccgctt
     1021 ccacctccac ctacgccact agaagaagct ccagcaccac cataccaacc accaccgcca
     1081 gcataaacat taccaatacc accttttcca aatgaaccat tgccactatt aacagttaca
     1141 ccaccttcgt attgagtgcc accagtacaa gtagttccag caccaacacc catgccacct
     1201 gcaaatccac cacctaaacc aattgaataa aaagaagaag taccaataga acctccgccc
     1261 cctgctacaa ttatacgtga taataatcct tgctcatcat tccaaacacc tccaataagt
     1321 cttatatcgg tagctccgcc tccacatgtt gtaaatttag ggtctacacc ttcattagga
     1381 cctcttcccc ctccattaaa accagcccag tcgctaatgt tactaaactt tttgtaccca
     1441 ctttcaccag cataaacata taatatagtt tcttttttta atgtgagttc tcctttagaa
     1501 tagccacctt tagcacattc agtccactca tcaaaacgac cacctccact agcaccccaa
     1561 cattctaatt tatacttacc aggttttaat gtaacttttt tccctgctcc aatataatca
     1621 aaattccatt cagtctgcat tttctcactc tcctctctta aataggtaac atatcattta
     1681 ttgtatttat attaacctca ttaaattttt ttatctttgc aacaacttca tctattgccc
     1741 cttgaacatt tgtagcagta agattacttg ttgcattatt ataacttgtt ttctctgctg
     1801 ttgtttctat actatctaca ctagttttta cctcatttaa tgcactaact atatttgttt
     1861 tatctgttgt ttcaagtagt gttgtatcac ctattttatt attaatctct gttttagtag
     1921 tttctatgtt gcttgttaat tctgttttag ttgtatcaat tttagtatta acagtaccta
     1981 ttttagtttc taagtcttgt atatctttga gtgttgcaaa gattattgtt gggtcaattt
     2041 taagttctat attattaaca ttagatacaa caaaaatagt ttttaccttc atgtcaataa
     2101 ctgctccatc ttccacttta ggtttatatg ctgacctata ctttgatata gcaagtaaat
     2161 taccttcggt atctaaataa cctatctctc taactacaaa tccaccagca ctagcaggta
     2221 ttagactctc taaaatcaag caatttgtca tactttcatc agttttgacg tttcctactt
     2281 taccttccca aactacattt tttaatatgg tttgttcctc tgtaggatta tactcatttc
     2341 ctccaccatc tccaagttga atcttgacaa aatcaacttt tcctcctact agacttgcat
     2401 ttgctatctt agccttacct atgtctgtta atattgtgta gtaacttttc tctgtagcca
     2461 aactaccacc tcctatctaa taaatagtta tttcttggta tccaaaccca ttaccactaa
     2521 gtatattaat ctctccttgt gtttcaatat cactaggtga ccatgggtaa attgtaatct
     2581 catgccctgt gattgttgtt gctccaaaat tcatataatt gtctttgctt ataagcactc
     2641 tagtgtaatc tagtgtcatg ttacatggct taatattact tacaaaagaa tgaacttcct
     2701 caaaccaatc ttgatttcta gcatcactct caaggtgtat gttataagta gcattattaa
     2761 tagttaattc ataattgcct tctccaacta cattatctag ccagttcctt aaaaatctct
     2821 ctgagtaagg tagtttactt atatatttac taaaaatcct aaaccttcta tcttctaaac
     2881 tctcattact tttaggagtt atagacatta tcttttccca tctttttata ccacttatag
     2941 ttaggtcctc taaaaactgg tcatttgata ggtcctttaa tttatcatgt aatgttttta
     3001 tttctttgtt ttctgcatta aatactttta tatattcttc tttatcttgt agaatttgtg
     3061 gcaagtaatt tattagatta atctctttat ccaactacct cacctctcac tactatactg
     3121 ttactatcta ttgttagatt agatttaaca tcatttatca ttgtgtttgt aatatctaac
     3181 actccatcta tacttaacaa acgagtttct atctgagata tacggactat taagttttct
     3241 tcatcttccc aactcatatt aagttcattt aaatagtcat caactgcttc ttctgcaatt
     3301 gtttttatat tctcccatgt atatccactc ttatatgtta tttctgctga tatatttata
     3361 gttgtacttg taactccttc aactgtgact cggtgtccaa ttggtgctaa tccaagacct
     3421 tctccttggt tttgtagagg gtcaatttct tcttgtacta ggttaactaa atcactagat
     3481 ggtactttaa aattagagtt aattattact aatttaacag tccctccacc attccaaaca
     3541 ggataaacct taactcctcc aacatcctgt atcttgttaa cttcatcctt atagttttgt
     3601 atatttccac caaatgactg agaattcaaa ctattataat atctttgtct taaactgttc
     3661 tcagattctt catcttctcc atttatcaat atttctgtca gttctgctgt ttctaatttg
     3721 tctacatatt caattggaat caactgacca agttcaaaaa taggtccagt agtttcgcat
     3781 ttcattttat atataccttc gctaatcctt tcagttgcaa tataattgta ttctcctagg
     3841 ttgaatcttg aatcaagagg aatatctata ttgaaaacac ctttagcaat cgtatgagtg
     3901 gctggtaaag gtgtgattcc tcgctctttg catcttttct ctaaataata gtaactagca
     3961 gtatcaacaa aagtttggtc taacatttca tccattgcaa tgtatgtttc tgtaagctcc
     4021 gctgcaacag gtgccaaggc attatatatt atagaacctt ctcttttatc aaaagtatct
     4081 ggtacactat ctaacattct tttaattata ttttcaaatg tcataagttc aaacaattat
     4141 acactcacca ccttctctgc ttttatattt ccatatttac tgtaaactgt gaacttacaa
     4201 tgtactttac cctttacatt ttgaaactca aaattatcta cattttcaac cctatcatct
     4261 tgaattagtg cttctttgat tcgtctttca agttcgggta ttacaaagga tataggctct
     4321 ccaataaggt cgttcaactc gactccataa tttctactgt atattaaatg ctcatacctc
     4381 tctgtgttta aaattaaaaa gatggtttgt tttaatgctt caacatcatc acaaatacca
     4441 tctatcttag atttttctat atttaattta aaggtcttac ttggttcttg ccttacatca
     4501 aaattaatta ttgagacatc ttcaatgtca taatctaaat tatcgcttgg taacacttca
     4561 tcacatccta tccaatacta aatattgttg tcctccttgc atacgaatta agactacttt
     4621 atctcctatt tttttatctg tatatctttt aaatgtatct gtctgtatta aaaaaaattc
     4681 ttcaaaggat gctttttgct ctatcttaac tactaaagga ttaacacttt ctatagttcc
     4741 aaatgcaaga gtcattggat tacttgtttc tactgcatcc attgcagcct tcttgattac
     4801 ttgtaataat tcttgtgaca ctttatcacc tcacttatat aaatcttctt acatgtgtgt
     4861 acgcttttcc ttttctataa gaattaacag actctatttt taccacatct cctgtttgtg
     4921 gtgaatgaat tatttgattg tttccaatat acattacaac atgattacta ctacctccac
     4981 caattctaca taataagtct cctgctttcc acttacttct atcttttaaa tctactgctt
     5041 ttcctgcttt actttgtgca gaagcagtac gaggaatttt tatacctatt tgtttataac
     5101 accattgagt aaatccactg caatcaaaag tattaggtcc tgtagctcca tacacataat
     5161 tacaacccag tttacttttt gctacactaa ttagtttatc tgctttagag ttgttatttg
     5221 tagatgtatt agcattgtta ttgttttgaa cttgataagt tgtatctttt aaattctttt
     5281 ctgcttcttc attgctccca actcctgtac ctgcactatt agaactatat gcatttcctg
     5341 ttatttgttt ataaaatttg cctactcctg gtacccaatc tttattaaga ggact
//
LOCUS       DAEMHZ010000192         5385 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000192 DAEMHZ010000000
VERSION     DAEMHZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.193"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            174..1637
                     /locus_tag="KPK72_003571"
     CDS             174..1637
                     /locus_tag="KPK72_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2490740.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1693..2829
                     /locus_tag="KPK72_003572"
     CDS             1693..2829
                     /locus_tag="KPK72_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2490741.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3065..4180
                     /locus_tag="KPK72_003573"
     CDS             3065..4180
                     /locus_tag="KPK72_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG2490742.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEILEGQINKSNVVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAIIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4332..5201
                     /locus_tag="KPK72_003574"
     CDS             4332..5201
                     /locus_tag="KPK72_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2490743.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2082 a          547 c         1006 g         1750 t
ORIGIN      
        1 tgattaatac tcttgatttt agaaataaat atttgtagat tgtagattaa agataatata
       61 aataaataga aggtagattt tttagatata ttaatataaa gattatataa aatattgaaa
      121 tatagctgtt taaattattg tctataaata taaaatgaat ggtggtgagt taagtgaccg
      181 atttagttaa caccattaat ggaattgttt ggtcacccgt attagttgca atgtgtttgt
      241 gtataggatt atatttttca ttgaaattaa aattttttca aataagagat gtaaaagaaa
      301 tgtttactct cttgcttgaa ggagatagtt ctcatgttgg tatatcatca tttcaaggtt
      361 ttgctacatc tatggctgga agaataggga ctggaaatat agctggtgta gctgtagcaa
      421 tagcaatggg aggtccagga gcattagtat ggatgtgtat aatggctaca ttaggttcag
      481 caactgcatt tgtagaatct actttagctc aaatatataa agatgagcat gatgggcagt
      541 atagaggagg acctccatat tattttgaaa aaggtcttgg gtggaaaaag tgttcagttt
      601 tattttcaat agttgcaata ataagttata cattttttat gcctggtaca caatcaaaca
      661 ctttatatct agcaatgaat caagcatttg gattttcaaa aatgacaata gcagtagcat
      721 cttctattgc attagcgata ataatttttg gaggaattaa aaggattggt ttatttgctg
      781 aaaaagtagt acctataatg ggtggagcat accttattat gacattagta ataattataa
      841 caaatattag tttagttcca gaggctattg taacaatgtt taaatctgca tttggatttg
      901 gttcagtttt tggaggaatg ctaggttcag caatatcttg gggagtaaaa agaggtatat
      961 actcaaatga agcaggagaa ggttctggga cttatggtgc ggcagcagca gaagtaagtc
     1021 atccagccaa acaaggatta gttcaagcat tttctgttta tgtagacaca ttattaatat
     1081 gtatggctac aggtattatg attgtaataa caggaatgta taatgttaca ggtgtttgca
     1141 cagcactacc aggtgtagaa ccgggaccac aatatgtaca gagtgcaata aatacattac
     1201 atccacaatt aggtgatatt tttatagcaa tagttttatt attatttaca tttacaactt
     1261 tattagcctt ctcatatatg atggagacta atgtatctta cttagtaact agaattaaag
     1321 cagaaaaaca tgaaaagtac ataattaatg tctgtagatt tatgcttata gcatgtgcag
     1381 caatgtgttg ttttatagaa ccacttacaa gctgggcatt aggtgatata tgtataggtg
     1441 tattaacata tataaattta attgccatct ttacactaaa aaaaccagct ataaaggcat
     1501 attatgatta tataagacag aagaagatgg gaataaaaag gagtgataga acttttaatc
     1561 ctatagagtt agatataaga aatgctgagt tttgggaaaa tagatactta aaagaggata
     1621 agcatgagtt aaattaaagt tataataaaa tataaaaatt actaaataaa tttagtggga
     1681 ggtaagattg gtatgaaaga aagaataata caagtggctg attcaaagaa agaaaaaatt
     1741 ctagggctat gtcaatcttt atatgatgaa ccagaaattg ctttgcaaga atataaatct
     1801 gctaaaaaaa tatcagaatt tctaagagaa gagggttttg atgttgagga aaacttggca
     1861 ggtatggcta cagcttttaa agcaaccaag aaaaatggag atggtcctaa aatagcattt
     1921 atagcagaat atgatgctct tccaggcaat ggacatgcat gtggacatca tttaattgca
     1981 tctatgggag ttggagctgg aatagctctt tcaagtatac tggatactta caagggagaa
     2041 gtaagtataa ttggaacacc tgctgaagaa actggagatg gaaagcctta cttgatagaa
     2101 catggtgtat ttgatggcta tgatgcagca atgatgatac atccaaattc aaaaacttgt
     2161 gtaacacctg aaattatagc aataggaggt ctagatttca tatttactgg aaaagcatct
     2221 catgcaggag ctaagccata taacggaata aatgctttag atgcagtagt actactatat
     2281 aataatataa atgcactaag acaacaatta gtagatggaa cgagaataca tggtattata
     2341 ctagaagcag gaactgctgc aaatgtaata cctgatatgg gaaaagtaag attagagata
     2401 agggcaaaag aacaaaatta ctttgatgaa gtagtagaaa aagtgaaaaa ttgtgctaga
     2461 ggtgctgcaa tagccactgg atgtgaactt gaattttatc actttgaacc aacttgccaa
     2521 ggattaaatg aaaataaagt tttggtagac atatttacaa agattatgga ggaatttgga
     2581 atatatgaag atgaacaagt aatgttaggt tcaacagata tgggaaattt aagtcaaata
     2641 atgccatgta tacatccttt gatgaagttt tctgaaaatg gagaagaact acatacaaaa
     2701 gaatttttgg aggcttctat aaattcatat gcaaaagata gagtaattga tggtataaaa
     2761 atattagctt taacaggatt taatttattt gaaaatccag agttattgaa aaaaatgaag
     2821 gaagagtaga acttagacta tttttaatta tactctttaa aataagaaga gtgtctcaaa
     2881 atgaactttt tagtttatga gacactcttt tttgtatgaa attaaatata tttttattat
     2941 tttaacaatg aaaaagaggc ttatctgttt ttatataacc ttttttaact tgtagcacaa
     3001 aatatcatga atgattacaa ttcgagtgat aaaatcgatt ataagatata taggaggggt
     3061 acatatggaa actttagaag taagaaatat tgaattgagt ggtacaaatt atgagatagg
     3121 atacagacta ggagaattgg ttgctaatat gccagagata ctagaaggtc agattaataa
     3181 atctaatgtg gtaagcaaaa aagaagaaaa agagatgata gagttgtttg ataaatattg
     3241 tccaggtctt aatgaagaat tgcaaggttt tgctgatgca attcaagtga attgtaatca
     3301 aattttatat tatactatga cttatcttaa accaggttgt agtcaagttg cattagcacc
     3361 agaattaact gaaaatgggc atgtactatt tgcacgtaat ttcgatttct cacacaatat
     3421 ggaggatttt gttttatgta aaactaaggt aaatggaaaa tatgcacata taggtactac
     3481 cgttatgcaa tttggaagag gtgaaggtat gaatgaatgt ggcttaggtg taagtcagtc
     3541 atcatgtggt attcctgtag gcaattcaga tggtttaaga aaaccagcaa ttgtaggttt
     3601 acagttttgg gctattatta gatatttgtt agaaaattgc aaagatgtag atgaagcatt
     3661 agaatattta aaggatatgc caattgcata caatattaat ttattgcttg cagataagtc
     3721 tggaaacatt gcacttgtgg agactttaga tgggaaaaaa gaagtaaata taatcaatag
     3781 tttagaaagt aaaagagaat attttttaca ctctactaat catacacata ttgataaact
     3841 tcataaatta gagccacaat caatgaaaaa ttctattcat agatataaat taattaaaga
     3901 atatataaat aagtctaaaa aaattggaga gaaagagtta atgaatttat tgtcttcaaa
     3961 atatccaaat ggcttatctt gtaattacta taatgatttt tttggaactt taaaaagtat
     4021 agttatggat ttaaatatag gaaagtttaa tattttatgg ggtggactag agaataaatg
     4081 ggagagctac tatttaaaaa atgacattaa atctataaca cagaggataa acataaatat
     4141 tgaaaaagca ccatctgact tttttgattt cattgaataa aaaatgtatt caattattct
     4201 aaataattag gacatataaa tcaactaaaa gtttttattt atcgtattga attagttata
     4261 atattttagt atagtaaaaa atagtgctat tttacatatt taaagagttt aagtagaaga
     4321 gaggtgccat attggattac ttaaagtatt ttgaaaaagc tgtaatgtat attgagaata
     4381 atttacatga aaatataact gtaaatgatg tggcaaaaga aactggatat tcttattatc
     4441 atttaacacg cttatttaag tctatgtttg gtgaaagtgt aggaagttat ataaaaaaaa
     4501 gacgattagt aagtagtaca aaagaacttt tatatagtga taaaaaagtg atagatatag
     4561 ctattggtag tggatttgaa tcttcagaag catttagcag agcatttaaa tctatataca
     4621 aggttagccc tatagaatat agaaaaaata gaattgatgt atttgttggt aaaaagaaaa
     4681 aattagagtt agattttatg agacatcttg ttggaaatat aacgattaaa ccagttataa
     4741 aagaaataga aaaaataaag gtaataggta taaaggataa agtaatttta gaaaataatt
     4801 cattacctga cttgtgggag aaattcagaa aagtacatca tattgtccca aatactttgc
     4861 catcaaagag agtttttgga atctgtgaag caatctctga aatacaccta gttagtgaaa
     4921 gtatggaatt taatgagata ataggacttg aagttaatag ttatgatgtg attccaaatt
     4981 catttgtttc aaaaactatt aaggaaggaa agtatgctat atttactcat actggtagct
     5041 tagataattt agacaaaact tatgaatata tatggggtac ttggttttta agttcaaaag
     5101 aagaacttga tataagagat gattttgaag tatatgatga acgattttta ggtcctagta
     5161 atgtgaactc acaaattgat atttatatac ctataaaata atctataaga aaactaaaaa
     5221 ataatttgat taagaaatag atgttcgtaa aagagcatct attttttatt tttatcagat
     5281 ttagataatt ttaataataa aagaaataaa aataagttct taaatttcta agaagaattt
     5341 agaatatagt cgaaataaga attgtatatt tctgaaaatt ctaat
//
LOCUS       DAEMHZ010000193         5358 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000193 DAEMHZ010000000
VERSION     DAEMHZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.194"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(213..1397)
                     /locus_tag="KPK72_003575"
     CDS             complement(213..1397)
                     /locus_tag="KPK72_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2490744.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1770..4121
                     /locus_tag="KPK72_003576"
     CDS             1770..4121
                     /locus_tag="KPK72_003576"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG2490745.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKKKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4143..4682
                     /gene="nrdG"
                     /locus_tag="KPK72_003577"
     CDS             4143..4682
                     /gene="nrdG"
                     /locus_tag="KPK72_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG2490746.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2095 a          599 c          852 g         1812 t
ORIGIN      
        1 ttataataaa tttagaattt agcttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttatat tacttacttt atatattaat taccattaat
      181 acattatatc taattactat tcatctaaat tattatgcca ttattttata tacaaattct
      241 tttaatttat ctaatccaga taagaaagta ttttcactgc aagcatagga tatacgaata
      301 tatttatcca taccaaatgc tatgccagga acaactgcaa cattatattc ttctaaaaat
      361 tgattacaaa attctataga aaaactatct ttatattcaa atttctcaga tactttagaa
      421 agatctataa atatatagaa tgcgccattt ggattcacat atcctacatt atcaatagag
      481 tctagttttg atttaattaa atctcttctt gatttatatg ttttaaccat attgtctatg
      541 tcaattgagc aatctttcaa ggctccatag gctatgtatt gtgctgttag acttgggtga
      601 gatataagat gtccttgtat agaactcatt gctttagcaa tcgttttatt agatgcagta
      661 tatcctaatc ttaatccagt cattgcagct gacttagaaa aaccatttat agttattgtt
      721 atatctttag cttcttcgct taatgatgca attgatgtaa attcaccagt gtagcatatt
      781 ttttcatata tttcatctgc taaaatatat attttatttt gtatgcatac atctactatt
      841 tctattaatt catcttttgt ataaacacta ccagtaggat ttgaaggatt gtttattaca
      901 agaatttttg ttttatctgt tatagatttt tcaagttctt cttttgttaa cttaaatcca
      961 ttttcttttt tagtatcaat aaaaactgga acagcattaa ctaacttaat catttcagga
     1021 taactaaccc aataaggctt tggtaataag acttcatctc cttcgtctgt taaagctagt
     1081 aaagtatttg ttattgagtt ttttgctcca cttgatacaa ctatttcgtc tattgaatag
     1141 ttacagttat tttcttcaat aagtttttta cagatttcct ctctaagtat ttttaatcct
     1201 ggaactaaat catatttagt gtaatcttta tttaaagaat caataccata agattttgcg
     1261 ttattaggta cattaaaatc gggttctcct atactaagat ttattacttt aactccatta
     1321 ctttccattt ctttaacttt agaactaata cctattgtgt aggatggtgt tataaagttt
     1381 agtctttttg ataacataaa atgcctcctt ttaaattatt atttatatta tacgacaaat
     1441 ttaaagaatt tacataagga aatttgtaga tatatacatt aatatatata atagcctaca
     1501 ctttttgcct aaaattagtg taaaaaaaag agaaaaaaat tttaactttc aaagttgtta
     1561 gaaacgctac tgttcaaaat atccacatag tatttgcaat gatttgcgca agtttgacta
     1621 ttatgtacaa atttggtaat cttaatatag caattattaa gataatccct ttaacttagt
     1681 tgaaggggat ttttttagac ttaacatact acatatagtg gtatggggat taataaaata
     1741 caatatatag taacaggagg tttttttaat tggttgaatt cgtaaaaaaa agagatggaa
     1801 gagtaattcc atttaatgaa gatagaataa caagagctat atttttagca gcaacgaatg
     1861 ttgcagaaag agaaggaata gtacctgatt ataaattatc tgagcaatta actcaagaag
     1921 taataaagtt tttaaatcat aaatactcag aatcagttcc aagtgttgaa gatatacaag
     1981 attctgtagt taaggttctt atagaaactg gccatgcaaa aactagtgaa gaatatataa
     2041 tatacagaac tgaaagaagt agaataagaa attcaaaaac aagattgatg aaggcaatag
     2101 aggaaattac ctttgaagat gcagaagatg ctgatataaa aagagaaaat gcaaatatta
     2161 atggtaatac agcaatggga actatgttac aatatggaag cactgtatct aaagaatttt
     2221 gtaaaacaca tatattaaaa ccagagcatt cttttgctca tgataatgga gatatacaca
     2281 tacatgatat ggatttttta aatatgggaa cattaacttg ttgtcaaata gatgttaaaa
     2341 aactattcaa tggtggattt tcaacaggac atggcttttt aagagagcca caagatataa
     2401 taagttatgg tgcattagct gcaattgcta tacaaagcaa tcaaaacgat caacatggag
     2461 gtcaaagtat accatttttc gattatggat tggctgaagg tgtatataag acatttaaga
     2521 aattctacat aggaaattta gctaaagcat tgaagttatt taaaggaatt gaaaataatg
     2581 atgtgataaa gaacatagta tataatacag agaaagaaac aaatcaaaaa gttggtctta
     2641 agagagatga attgtatcta aacttagaaa aaaagaaatt aatacagact tttgatatag
     2701 atgatgaact tgtaaataag atgcaaaact ttgcatttga agaatcgtat agagaaactg
     2761 ataaaaaaac atatcaatct atggaagcat ttatacacaa tctaaacaca atgcactcta
     2821 gagcaggtgc acaggtacca ttttcaagtg taaacttcgg aacagatact tctgaagaag
     2881 gaagaatggt aactaagaat cttctattat ctcaagaaag aggtttaggt aatggagaga
     2941 caccaatttt tccaatttta atatttaagg tcaaagaagg cataaattta aatcctgaag
     3001 accctaatta tgatttattt aaattatcat gcagagtatc agctaaaaga ctattcccta
     3061 attttagctt tttagatgcg ccttttaatg caaaatatta taaaaaaggt gaaccagata
     3121 cagaggctac atatatgggt tgtagaacaa gagtacttag taatgtgtgt ggttcagaaa
     3181 cagttagtgg tagaggaaac atatctttta ctacagtaaa tttaccaaga ttgggtataa
     3241 aacatggtat tataaataat gaaaaggcta atttagatgg tttctttgaa gaattagatg
     3301 agaagataaa cttaattata gaacaattat tggagcgcct tgaggttcaa ggaaataaaa
     3361 agatgaagaa ttttccattc ttaatggggc aaggtgtatg gaaaggttct gatgatttag
     3421 gaccagaaga tacactaaaa gaagtaataa aacaaggaac attgacaatt ggatttatag
     3481 gtttagcaga gtgccttatt gctcttatag gtaaacatca tggagaaagt aaagaagctc
     3541 aagaattagg attaaaaata gtgtcacata tgagacataa aatggatgaa gcaacggaca
     3601 aatataaatt aaatttctcc ttaatgggta ctccagcaga aggtctttca ggtaggttta
     3661 ctaaaataga taaaaaagtt tatggagaaa ttaaaggtat cacagataaa gaatattata
     3721 ctaattcatt ccatgtacca gtttattaca atataagtgc atatgataaa attgagatag
     3781 aagctccata ccatgaattg actaatgcag gtcatataac atatgtagag ttagatggag
     3841 atccatctga taatttagaa gcttttgaaa ctgttataaa agctatgaaa gatttgggta
     3901 taggatatgg aagtataaat catcctgtag atagagaccc aatatgcggt ttttcaggtg
     3961 taataacaag taatatatgc cctgtatgtg gaagaaatga agatgaaagt gacatcaaat
     4021 tcgaaagaat aagaagaata acaggatact tagttggtac agttgataga tttaataatg
     4081 ctaaaaaagc agaagtaaga gatagagtaa aacatagata gttttatttg ggggaataag
     4141 ttatgaaaat aagaatgtca tctactataa gttatgacag catagttgat ggaccaggat
     4201 taagaatggt aatatggact caaggatgta ttcataattg taaagagtgc cataatcctc
     4261 agacacatga tttatgtgga ggattttata tggatacaga agaaattata aataaagtca
     4321 aatcattgaa attacaaaaa ggcattacgc tatcaggagg agaaccattt ttacaaccag
     4381 aaccattaga agaaatagca agagaagcta aaataaatgg gttagatgta tggtcatata
     4441 ctggatttac gtttgaacaa ctgttagata aaaaaaatag agcgtatttt aaaaatttaa
     4501 atttactaaa gcaaattgat attttagtgg atggaaaatt tatagctgaa aaaaaagata
     4561 taagcttaaa gtttagaggg tcatcaaatc aaaggataat tgatgttcaa aaaagtttga
     4621 aatataaaaa agtgtttttg gttgagcaat atatgaagga tgatttatct atagcagaat
     4681 aaaatataaa taaacaggat taaatatatg catatattta atcctgttta ttttcatata
     4741 atatttcaag cttgtataat gtaacaatta aaccaaaaga ctcattgtat atttgaaatt
     4801 aagtttttta tagtttatag gtgttttatg tggattacga atgatatagt atcgatacat
     4861 tatttaatag attcttatgc caagaaacaa ttaatttata gaaattctta attgttacaa
     4921 tataaatatc tagataagtt ataagatact gataaataat ttataaagtt tattaatcta
     4981 atattgtgtg tgaatattaa gtggtcaagt agtctaaaaa atgaaaaaga tttaaaaaag
     5041 tgttttaact aaacacataa gtgttaaatt catgtataag tataggttag atacataaga
     5101 aataattatg taatatctat ctttgtaaaa aaatattaaa ttttgtaatt tgtggataaa
     5161 tatgtttata atgtggataa tatttaatgt aataagttaa agcaatatga atcaactgtt
     5221 tataatgtgg ataatattat aaatgtatga ataattgcag tatttaaaaa caaaaaaagt
     5281 taaaaaagtt gttgactcta aaagaaaaag ttggtatagt aatacttgtc ctcaagaaaa
     5341 gggcaaacaa ataaagca
//
LOCUS       DAEMHZ010000194         5354 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000194 DAEMHZ010000000
VERSION     DAEMHZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.195"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            149..1954
                     /locus_tag="KPK72_003578"
     CDS             149..1954
                     /locus_tag="KPK72_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490747.1"
                     /translation="MLSKLKEFQQEMIKYTETAASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            2031..2568
                     /locus_tag="KPK72_003579"
                     /pseudo
     CDS             2031..2568
                     /locus_tag="KPK72_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            3200..4414
                     /gene="dpaL"
                     /locus_tag="KPK72_003580"
     CDS             3200..4414
                     /gene="dpaL"
                     /locus_tag="KPK72_003580"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2490748.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKSSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4764..>5354
                     /locus_tag="KPK72_003581"
     CDS             4764..>5354
                     /locus_tag="KPK72_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2490749.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         2213 a          542 c         1035 g         1564 t
ORIGIN      
        1 ataaaacaaa ctttttataa attaatattg tctgttttaa cgcaaatata taaataagta
       61 atgttttata tatattttaa agagtgacta tatgttaaaa tatagctaaa ctgataattt
      121 ggtaaattaa attctaatgg gggaaactat gttaagtaaa ttaaaagaat ttcaacaaga
      181 aatgataaaa tacactgaaa cagcagctag tgtgttagat gtagatattg aaatagttga
      241 cgatagatta ataagaatta gtggtacagg tctgtataaa tcaaagataa atgaaagcgt
      301 tgttacagaa ggatttattt atgacaatgt tatacagaca ggtcaggaat tagtcgtatt
      361 ggatatatgt gataatcagc tttgtataga atgttcccat tacatgaaat gcctaaataa
      421 agtaataata gcagttccaa taaagtataa taatcgaact ataggagtaa ttggtgctat
      481 tagtacagat aaaactaaaa aagtagaaat aagtgcaaaa atagataatt atttaaaatt
      541 tgtcaatcat atatgcgatt taatttctat gaaaatagaa gaacatgaag cgtcaaaaaa
      601 tagcagtaga aaaatggata tgatgataga aatcattgaa aatgttgaaa aaggcgttat
      661 aatactagac ataaatagca aaatatctta tataaacaat atagctctaa aaaaattaga
      721 tatagataaa aatataatag aaaatatagt aaatatagta tctgttgaat cttcctcaaa
      781 tggacatgaa ttactagaaa ttgatattga taacaaaatt tataatataa atgccaaaat
      841 aattccagta tatccatata taaatcaata cgacaaaata ataatatttg acaagacata
      901 tatcaatcat aaaggacatg gaaaagtgaa tagtggctgg ggaaatagtg atatagagtc
      961 tataattgga aattcagaag caatgttaaa agtaaaagag agaactaaga aattagcaaa
     1021 gtcaaattca actgtactta taactgggga aagtggtacg ggtaaagagc taattgctag
     1081 ggctatacat gctgaaggta gcagatggaa caagccattt attgcgataa actgtgctgc
     1141 tattccagaa aatctccttg aaagtgagct atttggctat ataaaaggtg catttagtgg
     1201 agctagtagt ggaggtaaag taggtaaatt tgaacttgca aatgaaggtg ttatatttct
     1261 tgatgaaata ggagatttgt caatgccttt acaagcaaag ttacttagag ttttacaaga
     1321 gagaaaattt gcgagaattg ggtcaaataa acttatagat ttagatataa gagtgattgc
     1381 tgctacaaat aaaaacttat tgaaattagt aaatgaaggt aagtttaggg atgatttata
     1441 ctatagattg aatgtaattc ctataaattt gccacctctt agggaaagaa aggatgatat
     1501 tgaggcaata atgatgaagt ttgcttctaa atattcttta gaattaggta ttcagttaaa
     1561 taaaatagaa gaaaatgtaa tgaatatgtt gattaactat aattggccgg gaaatataag
     1621 ggaactggaa aatgcagttg aatatatgat gaatttggtt ggagatgatg gaataatata
     1681 taaagatatg cttccattag atattttaaa ctattataat attaatggaa atatttataa
     1741 aaataaggat gtaaatataa tatttgaaga tgatatagtt ggagggatag tagagaatca
     1801 agaaaggata ctttcaatta aagaattaga gttgacttac ataaacaagt tgttgaataa
     1861 gtatggaaga gatacaaaga cgaagaagaa gatagcaaaa gacttaggca taggtttggc
     1921 tacactatat agaaaattgg aagaggaaca gtagtaatat aatattaaaa tgataggtat
     1981 ttgtgtgttt ttatagatag gtatttttac tttatggtgg aggtgttatt atgcaatatg
     2041 cgatagagct atattatgat aaaaaaacgg aaaagcaact ttttgattta tctaaaaaaa
     2101 tcgctgatga aaaaattagt acgaagtact tagaatggaa aacaagacct catcttactt
     2161 tggcttgttt caatgatgtg gatgaggctt gttgcataga caagctaaag gggtttgcac
     2221 aaaaccataa ggtaatacct gcttacattg gttcagtagg catgtttaat gatactaaaa
     2281 cggtgtttgt atcacctata atgaatagca atgtatcagt ttcagagaga attatatgag
     2341 gtactacaag attttgattc aaatggttgg gaatggtacc accccaatcg ttgggtaccc
     2401 cattgcacaa tagcacttac tggagatgat gaaagtgagg ctttttttcg ggcaagtgat
     2461 gtattactgc gtgagttcaa aaaaataagt ggtgagtttg tctcaatagg attggttaaa
     2521 attacatttc ctgtggaaga aatttttaca gtgaatatga ataggtaact ttaactctgt
     2581 tgtggatgtt atgaatattt tgtatagtgc atttcatcag ataaatgcga agttgcgaat
     2641 ttcgacagct caatagtcgc aagtagggca caatggtaca catattgcac ttttgcttat
     2701 tggggggacg accatgtaaa cttctgtaag aagctatgaa aagatttaag ccattttgga
     2761 ggctttgggt aataaagaat atttgggtaa taaagaatat ttgggtaata aagtatataa
     2821 atttatagag tttatgcgta agaaggttat agccatatat ttatagaaaa taaaagtagt
     2881 aatatatctg acaaaacaat agacatataa aaaataagag cataataaag gaagaattaa
     2941 gataaataaa cgatttctcg aattgataat tctcgtattg ataaaaattt cccaatatga
     3001 gaattttttc ttttataaat cttatttttc acactcaaaa tttataaact ttaaagaaat
     3061 tttataaaag tatgtaattc caacataaaa aaattgtttt tagaataaat aatattggca
     3121 cggtaattgc tttgctataa ggcacaaaga taaaaaatta aaatgacacc gaaaaatttg
     3181 aaaggggaat ataaaataaa tgagagaaat aaaatggaaa ttaaataact tacctaaatc
     3241 agaggaaaaa gaaaaaggaa ttgaattttt aaaagatgaa gaaattgcaa aggctaaagc
     3301 ttttcatgag agctttccac aatatgaaaa gactccttta gttaaattag ataacttagc
     3361 aaaatcacta ggcgtaagcg gaatatatgt taaagacgaa tcttatagat ttggcttaaa
     3421 cgcatttaaa gttttaggtg gttcttactc tatgggtaga tacttagctc aaagattaaa
     3481 tacagatata tctgagttag ggtatgataa attaacttca aaagaaataa aagaaaaatt
     3541 aggagaaata actttcttta cagctacaga tggaaatcat ggtagaggag ttgcttggac
     3601 agctaataaa ttaggtcaaa aatctgttgt attaatgcca aaaagttctt ctgaatttag
     3661 attaaataaa atcaaaggag aaggtgcaga tgctagtata acagacttaa actatgatga
     3721 tgcagtaaga ttagctaatg attatgcaga agctgatgac catggagtaa tggttcaaga
     3781 tacagcatgg gacggatatg aagaaatacc agcatggata atgcaaggat atggaactat
     3841 ggctaaagaa gctatagaac aattaaaaga atatggagta gatagaccaa cacacgtatt
     3901 tgttcaagca ggagttggtt cacttgctgg tgcagttcaa gggtatgttg cttcaatata
     3961 tgatgaatgc ccaataacag tagtagtaga ggctgatgag gctgattgtt actataaatc
     4021 agcagaagct ggagatggaa aaccaagatt tgttggagga gatatgccaa ctataatggc
     4081 agggcttgct tgtggagaac caaacactat aggttttgaa gtgttaaaaa attattcatc
     4141 agcattcgtt tcagcaccag actgggtatc tgctaaaggt atgagaatat taggaaaccc
     4201 tctaagagga gatgagaaag taatctctgg agaatctgga gcagttacaa ctggagcatt
     4261 agtttcaatc cttgaaagtg aagatttaaa agacttaaga gaagctttga aactagatga
     4321 gaactctaaa gtattattaa taagtacaga aggagatact gaccctgata aatatagaga
     4381 tatagtttgg aatggtgaat gccaaagtaa ataaaagact taaggaaaag aaaaacttaa
     4441 caaataaatg aaaaatttaa ggaaaataaa aacttaaata agtgaaaact ttaaataaaa
     4501 taaaaactaa aacaaatgaa aaatctaaat aaatgaaaaa gtaaagcaaa taaaaaagta
     4561 aagcaaatga aaaattcaaa ataaaaacta aataaaatta aataaaacca aataaaatta
     4621 aaataaagtc taaataaaaa tttaataaat aaaatataaa gaaataaaaa atcaatagat
     4681 taagtgaaat gctaataaat taaaaaagtg aaatatcaaa ataaaacgtc aacaaattaa
     4741 aaattatatg ggggaatatc aaaatgttaa atgaaacaag aaaaaaacaa ttaactgaag
     4801 tatgccaaga cttaataaga aatgctagtt attcaggtca agaagaaaac gtagtaaaag
     4861 ctatagaaga aaattttgct ttactaggtt ttgatagttg gtcaagagat agatatggaa
     4921 atatagtagg atgtataaaa ggaaataaac caggtaaaaa aatattattt gacgggcata
     4981 tagatacagt tccagttcca gatgcaagta aatggagtgt tcctcctttt gaagcgagaa
     5041 tagtagatgg gaaaatatat ggtagaggaa cttctgatat gaaggggcaa gtaagtgcta
     5101 tgatggctgc tgtttcatat tttgcagaag atactaacaa agactttgaa ggtgaattgt
     5161 atgtagcagg tgttgttcat gaagaaatat ttgaaggtgt ttcagctaga gagataagta
     5221 aggctgttca acctgattat gttgtaatag gtgaatcttc agaattaaac ttaaagatag
     5281 gtcaaagagg tagaggagaa atagtagtag aaacttttgg taaaccagct cactctgcta
     5341 atccagaaaa aggt
//
LOCUS       DAEMHZ010000195         5295 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000195 DAEMHZ010000000
VERSION     DAEMHZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.196"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(85..969)
                     /locus_tag="KPK72_003582"
     CDS             complement(85..969)
                     /locus_tag="KPK72_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2490750.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(1260..1736)
                     /locus_tag="KPK72_003583"
     CDS             complement(1260..1736)
                     /locus_tag="KPK72_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG2490751.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2003..3178
                     /locus_tag="KPK72_003584"
     CDS             2003..3178
                     /locus_tag="KPK72_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG2490752.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(3259..4062)
                     /gene="proC"
                     /locus_tag="KPK72_003585"
     CDS             complement(3259..4062)
                     /gene="proC"
                     /locus_tag="KPK72_003585"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2490753.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4102..4686)
                     /locus_tag="KPK72_003586"
     CDS             complement(4102..4686)
                     /locus_tag="KPK72_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490754.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1751 a          884 c          599 g         2061 t
ORIGIN      
        1 ataaacagat tttataaaaa aataattcct aagtagagat taacatgctt tctcatactc
       61 tcatatataa tgtgtctaaa aatattaata aaaatgtttt aattgtttta acttacttgg
      121 atgtcttaat tttcttattg ctttagcttc tatctgtctt attcgttctc tggtaactcc
      181 aaataccttt ccaatctgtt ctagtgtttt agggtcatca tcatcaattc caaatctcat
      241 tcttaaaact tgctgttctt gctctccaag acctgtaagt agttcttcta tcttttcttt
      301 taaattatgc tgttcaactt cgtgtattac aacatcttta aaatcagcat ctgatggtat
      361 aaattcaact aaactactat cctcatcttc ttttaatgga gtatccaaag atacaacatt
      421 tttatctctt cttaataatt ccaacacttt gtcaacttct aagccacatg catctgcaat
      481 ctcatctata gttggctctc ttaataatac atttaaaagt tcttgtttct ttttctttac
      541 aaaatttatt ctctgatgaa gatgtatagg tatcctaata gtattttcgc aatcgtcgat
      601 atatcttgtt atactttgtt ttatccacca agttgcataa gtgctaaatt tgtatccttt
      661 tttataatcg tacttttcta cagcttttat aagtcctata tttcctgctt gaattaaatc
      721 caacatatca atactatctc ttttatatct ctttgctatg ctcactacca acctataatt
      781 tgaatttata aatttttctt ttgctactga agatgagttg attatttcct tagccagctc
      841 tacctcttct cctgctgaca acattttgta ttcctctatt tcctttaaat acattttcat
      901 cgcatttgat acatttgatg aacccacaca aatataattt tcataattat tttctttttt
      961 agtatccata atcaaacccc ttctattcat agaatttgct aaaacaaaaa taaataattt
     1021 aaacctacat aatctacaat tacataatat cccattcttt aaatgaattg ttttgtatat
     1081 tatgtataca aatttataaa aactatacct ataatattat tctacttcta acagcaatat
     1141 cctccttata tatataattt attttattat atgttgttat attcttagtt ttttgaatga
     1201 tatatgtcta aaaaaagact gtttactaaa cagtcttttt ttatatatat gctcaatatt
     1261 taattttcat gatgatgatt acatccacat ccacaatttt catcatggtg atgatgttct
     1321 tctaaatctt ttacctgttg ttccatttca tgaaactctt ttaccattat atcataagtt
     1381 ggaagagctg cctcagtatc gtattcttcc cctctccaat cagcgtacat catagcatcc
     1441 ccattagtta aaaccaatct acctgacata gttatgttat ccacttcttc taaaaattct
     1501 atttgttttt gtaaaacttc tccttctaac tccacatctt catctaaaca ttgcactatc
     1561 attacaggtg cataataatc ttcttcatct ttaacattta caacaatatc taaaattcct
     1621 tctgcacctt cttcaaattc tgccaactca acatttgtat caatcatatc ttgaggtact
     1681 aataaatctt ctattaaata ttttattact ctatctttta ctaattcttt tgacatttat
     1741 ttttctcctt ccgattctat aacattatca taatcatttc tatattatag tatactacat
     1801 taattgtttt ttcaatctct tacaaaaatt atttttttat tatcttcctt atattccctt
     1861 ttttattaca aagttgtaat cgcattgatt taaaaaatag tttatattta gtataataga
     1921 aatgtagcac atatttttat tcaatatatt aataaaaaaa tatattgaaa atgtaatgta
     1981 tgttaaatct aggaggaata ttatgaagtt taaaaaacaa attagttcta ttgttgtgtc
     2041 tactatgctt gtacttggga gtatgaacct ttcttatgca gacggtacaa atgtagttac
     2101 acttggagct aacttatcag agtctcaaaa gcagcaaatg cttaattact ttggagtaaa
     2161 aaaagaccaa gtcttagttt tagatgtaac aaatgcagaa gaaagacagt atttacaagg
     2221 tgttgcaaca gagggtcaac ttggaaaagt tactatctct tgctcatacg ttgaacctac
     2281 taaaaaagga aatggaatta atgttaaaac agcaaactta acttgggtaa ctagttcaat
     2341 gattgcttca acccttacaa cagctggtct tgaagatgca aatgttattg ctgctgctcc
     2401 atatcctgta agtggtactg gagcattgac aggaatcatg aaagcatttg aggatgcttc
     2461 aggaaagaaa cttgatgaaa ctaagaaaga aattgcttct gaagaattgg tagttacagg
     2521 tgacttagga gatgatatag ggcaagaaaa agcaacaggt gtaatgaatg atataaaaac
     2581 agaaattgta aagaatggta caaaagatac caatcaaata gcaaatacaa taaataatgt
     2641 tgtaaataat tacaatatta ctattacacc agaacaaaaa gaacaaatta aaggtgtaat
     2701 gaaaaaaata gctgaccaaa attacgatta caatagcatg aaaaatacac tagataatgt
     2761 atcagataat gtaaatgaac aattaaagaa attaggtgaa agtgttaagg gttctggtat
     2821 actagatact attggtggat ggtttagtgg aataggagac tggttctctg gtttattctc
     2881 aggcggagat aaaaaagatt tgggaatctt aaataacact aatgatgagt ctcttggctc
     2941 tgatgctgta ataaattcta cagaaggtgt tcaaataaat ccacaaaata atactaattc
     3001 taacaatgat agtgatacaa atagctcaaa aaatgatact tcaagtaata atgaaaataa
     3061 cgatagttct caaacaaatg aaaacaacac aacaaataat tcagaaaatc aatcaaataa
     3121 taatgactca gttggtttct ttgaaaaaat aaaaaattgg ctttcaggct tattttagaa
     3181 attaaacaaa taaaaagaat tgcctaaaat ggaaattttc tataaaaggc aattctcttt
     3241 atttacaata aatttttatt actcactcat ttttttagat ttttctatac atgcacatat
     3301 tgcttttatc acagaagctc taaaaccttc ttcttcaagt actttaactg cttctatagt
     3361 agttccacca ggtgaacata ccatatcttt taattcacca ggatgttttc ctgtctcaag
     3421 taccattttt gcagaaccca taacagcctg tgatgcaaac ttatatgctt gtggtcttgg
     3481 cattcctgca attactgctg catctgctat agcttctata aataaaaata catatgctgg
     3541 tgcagaacca cttgcaccaa ttacagcttc tataagatac tctggtacta cctcagtatt
     3601 tccaaaagaa ttaaatactt ctgtaactgc ttctaagtct tctttgttta tatttttatt
     3661 tatagaaaga gaagacatgg cttcatttac aagagctgga gtatttggca tagttctaac
     3721 tattttttta tcttctccta taatagactc tatatctttt atagattttc ctgctgctat
     3781 tgtaactata attttattgt catctataaa gtcttttatc tcttccaaaa tatcatcata
     3841 tatatctggt ttaacagcta caattactat atcagaggtt ctagttactt ccttcgagtc
     3901 ttttgtagta tttattccaa aatttgcatc tattctatcg agagttcctt gactatatgc
     3961 tgatgctata accatattag gttcaactaa ttttgaattt actattcccc ctatcatagc
     4021 gcttgccata tttcctgcac ctatgaatcc aatttttttc actttagtac ctccttattg
     4081 actaatagta atcgttaaaa tttaagcttc tttaactgca tcagactttt caaatggatt
     4141 tggtatactt actaccaaaa ttttcactac cttattagta tagttgaccc aattatgatt
     4201 gttcttttca ctattatagt gtattgtgtc tcctggatac atttctattt gttcatctcc
     4261 caaaaacact gtaagagtac cctctaatac atacacaaac tcctcacctt catgtacgta
     4321 acaacaaatt tcctcactac tcttacttgg aagaatttca attaatcttg gtagcattgt
     4381 tttttccttt aaactagatg ataaatgata atgtataaat gttgagtttt ctacatcaaa
     4441 tacttctttt tcataacttc ttaatacagc tcttttatgc tcttttggct tcataaaaaa
     4501 gtaagttaat tctacatcta gtacacttgc tatttttcct aaggaatctg ttgccacact
     4561 tgttaatcct ctttcaagtt gtgataaaaa accaattgac aagtttgttt tttcactcat
     4621 atccttcagc gtcatttgct tttgagttct gagttgcttt atttttgctc ctatgtcctt
     4681 attcaaaacc atcaccccta aataatttta attttttcat aatataaata caaaaatata
     4741 aatataggta ctgaacctat ctttatttca tttaaaaaaa tttttataag tattactata
     4801 atttatcaat ctttttttgt ctacattttt acgattattt acgatttatt tttcacatat
     4861 gtatatttaa tcttgccaac taaagataca ttttttataa gcatttactc tttatataat
     4921 aaaacttaag ttgttcatgt agtcttcttc tggtttaatt attaaaattt caaattatat
     4981 tattatttct acacattatt acatccttta gtacaattat aacataattt tacaatttaa
     5041 tgatatctat tcaatgtctt tttttactct acaacatatt ttattttaga gtttaatact
     5101 atatatattt tttatataca gccttgtcat tttttagatt gtcaagagtt attgaagtat
     5161 tttatgtaga tttagatttt atttcctaat ttctcattta acaagaatta cttatgataa
     5221 aaaataatat catctcaata agattcttgt aaatatatct atattctaaa tataatttct
     5281 ttttttgtca aaatt
//
LOCUS       DAEMHZ010000196         5259 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000196 DAEMHZ010000000
VERSION     DAEMHZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.197"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(401..1807)
                     /locus_tag="KPK72_003587"
     CDS             complement(401..1807)
                     /locus_tag="KPK72_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2490755.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEILHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1852..2559)
                     /locus_tag="KPK72_003588"
     CDS             complement(1852..2559)
                     /locus_tag="KPK72_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490756.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2730..3644
                     /locus_tag="KPK72_003589"
     CDS             2730..3644
                     /locus_tag="KPK72_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490757.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNKGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3644..4405
                     /locus_tag="KPK72_003590"
     CDS             3644..4405
                     /locus_tag="KPK72_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490758.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLSSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4417..5154
                     /locus_tag="KPK72_003591"
     CDS             4417..5154
                     /locus_tag="KPK72_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490759.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTAVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1806 a          810 c          691 g         1952 t
ORIGIN      
        1 attataacta cacagaacag cgtataatta aaactttttt aggatgtcat tatatttttt
       61 cttaaaatta aaaaacacta taccataatt ctattttagt agaatttttt tgtcaataat
      121 aaaattaaat attaactaaa aaataaaaaa aatggaatta tctaaagaat ttaataacct
      181 tacaataatt ccaaaatatt atttctatga aaattttgat accatgagta actcagccat
      241 aagcgcataa agcacaagtt actctttact ttaataaaac ttgacaagaa aagaatttat
      301 ccttattcta ctctaataga gttaatttgt caataaagaa taaaattatt ttcaaaatat
      361 tttcattctt cattcttatc tgttattatt ttataaaata tcacttaatt agaggtaatt
      421 ttagtattgt ttttagtccc tgactatcaa tgctcgtaat atctaattta cctctatgtg
      481 cattaataat gccagatact atcaaaatac ctagaccatg attttgacct ttggcatatg
      541 cctcacttag tccattttgg ctctttaata aactattaat tctttcttga ctcatacctt
      601 ttccatcatc ttcaacaatt atatagcaaa aatctaaatc agacttagag gtaaccctta
      661 tattacatcc ttgttggtta tgagttatac tattttgtat taaattacta atagcccttt
      721 ttaataattt tttatctgca tctataataa tatcttcatt tagtatatca agttcaacgt
      781 aaaagtcatt atccaaatta ttatttataa actcagaaat aagctctctc aaaaacgcag
      841 aaactctaat cttatcacaa ttcaatgttt gcatattata ttcaagttta gacactaaat
      901 tgaggtcatt tactaattct ctgattttaa tcccttgatt gcatataata gacacttgct
      961 cttgttctct atttgaaagg ctactgcttt cttcaagttc tccagcatat ccaattatca
     1021 ttgaaagtgg agttcttata tcatgagata ttcctgcaat ccagttagac ctagcttcct
     1081 ctttcttcct aagtttttca tctttcattt gcaactcttt agatacagta ttaatagatt
     1141 ttgaaatatc acttagtatt cctttttctt gtaaaagtac tggttctcct tttgacataa
     1201 gctttatgcc agtaataatt ggctttatag atttcaccat ttttaaacct atgaatagag
     1261 ctattaaaaa agtaactatt gcattaacca aaaacatacc tattattaac ataggaaatt
     1321 gttctatttc cttaaccgta taagtaagat taaacttagt gtacttgtct tttggataac
     1381 ctgttactaa tattccattt ggatgttccc atacatacac aggatagtcc tttaaaaaga
     1441 accttgaaaa ctttgctaca tctgtcaaag tataatgact tggaatttct tttggtaaat
     1501 caaatcccca tattctatct ccattgttat caattaacat tgcccaaact ttatttttat
     1561 gtagtatttc cttactagaa tcatctaaaa taaactttcc atcttttaaa accaaattat
     1621 cttcaatcat aatcaagttt gattttattt ctctgctatc acttgtatct aaattatcag
     1681 agtatttaga tacaaataac atatatgaaa acagaagaaa acctgctata tttaatacaa
     1741 taaaaagtaa tgaaatcaag atacttgaac ctataaatcg cctcaatatg tactttacac
     1801 tatccaaaat taaatcactc ctcttgattt cttttattct aatatctaaa attaattaat
     1861 catcaactta tatccaagac ctcttactgt aataagatgt ttaggtgtag atggagtttt
     1921 ttctatcttc tctctaactc ttctaacatg aaccataagg gtattttcat atccatacat
     1981 atcatctccc caaatggctt gacatagtga atcattagta acaattctcc ctttattttc
     2041 atacaacttt aaaagcaacg aatgctcttt agctgttaaa gaaatctctt gcccatcttt
     2101 ctcaacacat gcactattta aattcacaac aacagaatct cccagtttaa atactggcaa
     2161 ctcctcttct tctataggca catatacacg atttaatata cccacaagcc ttaaagtaag
     2221 ttcctttggt aaaaatggct ttactatata atcgtcagcc cctaagccca aaccaatcaa
     2281 tctatcttca tcttcacctc ttgcagagag aaaaatcact ggtattttag atgtctctct
     2341 aatttttctc ataatagaaa aaccatctcc atctggcaat acaacatcta aaattgcaca
     2401 atctggcttt acatttctaa ataccctaac agcttcctca taacttgatg ctaaaaatac
     2461 tctactaaat ccctcttttc tcaggatttc atctatcatt tttaataatt ccttgtggtc
     2521 atcaaccaca agtatttttt tatcttttat tgactccata atcatcaccc tcctttagta
     2581 taccatatac ccattttcac aactttgcct aaatagactt ttaaggttat tgtaaggtaa
     2641 atttcagcta attgaaatgt aaccaagtta ttatactgat ataagttaaa agaatcaata
     2701 agcacagatt aaaaaagggg atgaaacata tggaaatgat aattagaaca caaagacttt
     2761 caaaggaata caataaaata tttagagtaa aggatataga cctaagagtt ccacaaggag
     2821 ctgtatatgg ttttttgggt cctaatggag ctggaaaatc taccactctt aaaatgcttc
     2881 ttggacttgc taaacccaca caaggaaata ttaatatttt aggtaaagag ttaaatgaaa
     2941 aaaatagaat atcaatttta aaggatatag gttctttgat agaatctcca tcatactatg
     3001 gtcatcttac agggcttgaa aacatgacca taatgcaaag actcttagat ttacctaaaa
     3061 aaaatgtaaa tgaagcatta aaaattgtaa ggttagaaaa tcaaaaaaat aaaaaagtta
     3121 gtcaatattc tttaggtatg aagcaacgtc ttggtattgc tatggcaata atgaagtttc
     3181 ccaaattatt aatacttgat gaacctacaa atggactcga cccagcaggg attgaggaaa
     3241 ttagagaatt aataaaatct ctaccagaaa aatacgggat gactgttcta atctctagcc
     3301 atcttttaag tgaaattgac caaattgcca catcaatagg aataataaat catggcgaac
     3361 tggtttttca agatagcata aaagaacttc ataataaagg tcaaagtcag attgctatca
     3421 aaactaagaa tgttgtatct gcacaaaaac tattaaatac taaaggatat atgtcactta
     3481 tacaagatgg gtacttgact attgaaaatt taaaagactc agatgtcgct aaaataaatg
     3541 gtatgcttgt ccattcaaat atagatgtat ttagaataga agaacataaa aaaagcttgg
     3601 aagatatatt cttagaactt actggtaagg cggtgacttt ataatgaaat accttggtct
     3661 agaattttat aaacttaaaa gaaagaagat tattctgatg acctttttat ttgtagctgt
     3721 tgaaataatg tggtgtatcg taaattcaaa tagagctatt acacgtaatc cagatatgtt
     3781 aggtggattt gaatattcct atatgcttat gagttttgca agtttaaatg gtctattctt
     3841 tcctatctta atatcaataa ttacatcacg tataagcgat atagaacata aaggagatac
     3901 atggaagctt ttaaaatctt ctgtgacatc tttaagtagt atttatttat ctaaattttt
     3961 atgttctgct attttggtat ctatacctgt attgatgcaa gtgttatcta ttgtgttgtt
     4021 tggacatttt agaggggtta ttgagtcaat ttctgtatct ttacttttaa aatacacatt
     4081 aggaacaatt cttgtaagta ttgctatcat tgcactacac ctatggattg caactgtaat
     4141 tccaaatcaa atgattgcaa ttacatttgg catgattggt agttttatag gtttgacatc
     4201 aggtttgttt tatcaaggta ttcgtagatt gtttatatgg tcttattacc tagagttatc
     4261 acctttaagt tatgcttatg ataatgtttt aggttcatct gtctatagaa ttgatatgaa
     4321 tttttctatt acgattttag ttttcttaat tggaatgcta atttattata ttggaagaaa
     4381 tcatttatta aaaaaagaag tataggaggg atttaaatgt taaggaagtc agttttggga
     4441 gaaattctaa agttaaaaaa ttcttttata tggtatgtat tattagcttt gccacttata
     4501 agtgtgttga ttggttctgg taatttttat ctaaatcaag gcatactcaa aaaagaatgg
     4561 tacagcttat ggactcaagt tagtcttttt tatggggaat ttttctttcc tatcttaatc
     4621 gcaatctttt gtgcatatat ttgtaggctt gaacatatga atcataattg gaataatgta
     4681 atgacactgc cagttaattt aaaaaatatc tttttatcta aacttacagc agtttcaata
     4741 cttacagcta ttacacaagt tttctttgtt atcttttata tagttgcagg aaatatgttt
     4801 ggtttttctg gtagtgtacc taaagaattg actggttgga ttgctaaagg ttggattatt
     4861 tctatttcaa tagcttctat acaattatac atgtctataa agattagaag ttttgcaact
     4921 cctattggta ttagtctttg cctttctcta tttggtatgg gattaatagt agcaaaacct
     4981 gctgttggta tatttttccc ttattctatg ttaggtagtg ctatgggggt tataaagcaa
     5041 tctgctttaa atgttaatga cactatagtt acaatatgtg ttgcatttat tactactata
     5101 gtattttctg aaattgccaa tagaaatttt agaaagaatg atgttatttc ttaatggtaa
     5161 atgctagata ttttggggtt tgaaatttat attcttttta gttgaaatct atatccattt
     5221 tatttaaaaa tatatatctt ttatttgaat tttatatgt
//
LOCUS       DAEMHZ010000197         5237 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000197 DAEMHZ010000000
VERSION     DAEMHZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.198"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            47..1051
                     /locus_tag="KPK72_003592"
     CDS             47..1051
                     /locus_tag="KPK72_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2490760.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLKMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGVKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1177..2289
                     /locus_tag="KPK72_003593"
     CDS             1177..2289
                     /locus_tag="KPK72_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2490761.1"
                     /translation="MKIKRILMVCFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQNESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2779..3540
                     /locus_tag="KPK72_003594"
     CDS             2779..3540
                     /locus_tag="KPK72_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490762.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3543..4361
                     /locus_tag="KPK72_003595"
     CDS             3543..4361
                     /locus_tag="KPK72_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG2490763.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLREYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4541..5143
                     /locus_tag="KPK72_003596"
     CDS             4541..5143
                     /locus_tag="KPK72_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG2490764.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         2189 a          486 c          931 g         1631 t
ORIGIN      
        1 taaataggat ataaaaatat cgtaaatgta tgaggagtga aatttaatgc gtttcaaaag
       61 aatattgata ttgatgttaa ctgtagcaat gatagctgga atgttggttg ggtgtgctaa
      121 taacccaagc aataatccta gctctagcaa agataataaa agtgatataa gcgataaaag
      181 taattcaaaa aaatctgtat caatgatact agatattgaa ggaacaaata atgaagctat
      241 gaataattca gctttattag cattaaacaa tgctcaaaag aaacttaata tagatacaaa
      301 caaagtggaa tcagatgatt cctcaacttt ttctaatagt atagatattt tatgtaatga
      361 caattacgat ttaattatag ctgtaggagc tagatttgca aaaccattga agatggtagc
      421 caagaaatat ccaaagcaac aatttgcaat tatagactat gagtatgaca agcaaccttc
      481 taatataact tcaatttctt atgaagataa taaatctgga tacctagctg gattaattgc
      541 tggaaaaatg actgaaagcg ataaggttgg ttttatagga ggagtaaaga gctctagtag
      601 ggataaattt gaatcaggtt ttagagaggg tgttaaattt tctaatagta gtataaagga
      661 tatttcagtt gagtatgcag atgtttttaa agatagcaaa tcagtagaat ctatagctaa
      721 aaaaatgatg gataatggag tggatattat tttttctact actgaagatg attcaaaagc
      781 tgtaatagat gcagtaaggg caaaaaataa aaaagttata gcaacgaata aagaccaaca
      841 tgagttagct ccagaaaatg ttataagttc tattgtaaaa gattttgaga atcctactta
      901 taatctgata caaagttttg taaaaggaaa ttataaaggc ggaaaggtta tagaaaacac
      961 agttaagtct ggaagtactg gacttgccca aaatagttct caaaatattc ctccagatgt
     1021 gttagaatat gttaataaga acaataaata atctactata atatccaaga atacttatga
     1081 ctttagttat gaggtgaatt ggatgttata atagacatat atagaaagac atatgcagag
     1141 gtaggtcaga cctatctaaa tcagggagtg aattttgtga aaataaaaag gatattgatg
     1201 gtttgctttg tagtagtttt tacaattttt atattagcta tatgtaattt tgattttgga
     1261 tttgataaag taaataagaa taattctaat atagatagtg ctaaatcgaa acagaatgag
     1321 tctaaaagtt tgctaacttc agcagaaaag gataaatcaa atagcaaaag taagaataaa
     1381 gaagatgaca cagaagatga ttcatatgta agaaaaacta ttggagtatt gttagatacc
     1441 gaaaacttaa ataacgaatc atataataac aatgtactat cagccttaaa aagggctgac
     1501 gaagattttg atgttaggat aaaacttgaa aaaccaaaga cttctaaaag ttttaaagag
     1561 agtatagaaa aactgcataa gtatgaacca gatttgataa tagcagttgg agccagattt
     1621 gcagagccaa ttaaagaagc tgctactaaa tatcccaaac agcaatttgc aatagttgac
     1681 tatacttata aagttcagcc agctaatgta atttctgtat catttcaaga taatgttact
     1741 ggatatttag caggacttat tgcaggtaaa atgactgata ctgggaaagt cggttttata
     1801 ggaggagttg agggttctag tagagataag tatgaatctg gatttagaaa ggggcttaaa
     1861 acttcaaata aagatgctga gttactagtt aagtattcaa atatatttga aaaccctgaa
     1921 tcagctagaa ctattgcaaa ggaaatgaga gattctgata gagatgttgt attttcagct
     1981 acagaagatg atagtaaaag agctattcag gtagcaagag aaaatggtgg taaagttata
     2041 gctattaata aagaccaata cgatatggct ccagataatg taataagttc aattataaaa
     2101 gatgtagaac aacctgtcta tgaactgata aagaatgttg caaaagatgg ttttaaggga
     2161 agtaaagtaa tggaatttaa aataaaagat ggagaacttg gcttgacttc aaagagcagt
     2221 agaaatatac caccagatgt tttggaatac ataaaaaaag agtataacaa agtgaaagat
     2281 acatattaat tattgatgta gaagattata aaaatatact gcttaagttt agattagtaa
     2341 taaaaagata tatttattaa atttgtgcta actgtacaaa atatattgat taagtttaga
     2401 ttagtgataa aaagatatat ttattaaatt tgtgttaaca gtatggaata tactgcttaa
     2461 gtttagattg gtgataaaag gatatattta ttaaatttag tctaactgta caaaatatat
     2521 tgattgagtt tagattagca ataaaaagat atatttatta aatttgtgct aactgtacgg
     2581 aatatactgc ttaagtttag attagtgata aaaagatata tttattaaat ttgaaccaac
     2641 tgtacaaagc tatactaatt aaacttagat taactatata aaattataac aataagtgtg
     2701 tgggcagaaa aattctgcta cacactttat ttatattcac ataaaaaatt tatcaaaaaa
     2761 aagaaaaagg agtaaaaaat ggaatttaca agagaaccat ttatactaga tgacgatttg
     2821 tgtctagctt tggtggacaa aagaattagt tatctaatgg aaaatgaatt aaaattaaga
     2881 aatatagaga taataaaaac aatagaatgt aaagaattat atgaaagcat aaaatatcac
     2941 ccggatatat gtatttgtaa tttaggaaaa ggagatataa ttgttgcgcc gaatgtatat
     3001 aatcaataca aagagttatt aagtagatat aaatttaata taattaaggg agaagctaat
     3061 ttattaaata gataccctta tgatatacct tacaatattg cgatagttgg tgactatgct
     3121 atacacaatt ttaagtatac tgacaaagca atattagatt atattgaaaa taataaactt
     3181 aaaaagataa atatagagca gggctattct aaatgctcaa tctgtatagt agatagaacg
     3241 tctataataa catcagataa aggtatttgg aaaagtatga agaatacaga aatagagtgt
     3301 ttattgatag aaaaaggtca cataaattta tttgagatga attatggatt tataggtgga
     3361 tgtacagggt tgatttctaa ggataaacta gctttttgtg gagatgttaa aaaacatcct
     3421 gactatgaac gtataaaaag ttttgtagaa agtaaaaaca aggagatagt tacacttagc
     3481 tgtgaaaatt tactagatat aggttctata gttcctctca tgacaagaaa ggagcgataa
     3541 ctttggttga gaaggataat atttcttcca tgaaattttt gaggaataat aatgtaaacg
     3601 atagattaat atatacgaat gacattgtag aagtagtgat agattctgca aagcaagaag
     3661 tgttaaataa aaaaatactt aatggtacta aagctgaaca aagagcttta aatattgatg
     3721 agatatcaac ggaaataact agtatgataa taagtataat gaaagagaaa cttttgagag
     3781 aatacattct aaaatgctat gggcgtacat atcctgaaga taaaaataat atatacatat
     3841 attcactgaa catatttgct aaaaaagaga tttttatgag agaaacagta tttactaggg
     3901 tgagaaatta tatagaagaa aatgatttta taaatgtaga aggttttatt agatttagaa
     3961 tgagagaatt tatgaaatat atttctgcta taggtgatat tgcagttgag gagtatttaa
     4021 taaagaaaga ccaagatgaa tttataagag ttttaaaata ctttatagat actcaagaag
     4081 aaaagattga tttgcttaaa gttcatataa tggaagataa tacatttgtt ttatacgata
     4141 aaaatggaaa taagatagac agtatagatg atgaagaaat tataaatatg gtaataagag
     4201 aaaatttaaa ttatgaggac tttttaataa gtaccttatt aactctatgt cccaaaaaaa
     4261 tagaaatatt ggatttgtta aataataatt gctcaaagga aattatagat actgtagaag
     4321 caatttttga aaataaagtt agtataatta tggaaaacta gaatctaagc atataaaagc
     4381 tatccatata aaattaaaaa aattcttttt aaatcgacac aaaagactta taattgtata
     4441 taaagctata gtataactag tgttattaag tataaagaat atctacttga ttaatacaat
     4501 atagctggca aatatacaat tgataaggag taattttata atggataatt taattaggat
     4561 actgatatta gctgttattg gtggatttat aggttatgtt acgaatgtag ttgctataag
     4621 actgatattt agaccaatag agcctattaa aatacctatt ttaaatatag aaatagtagg
     4681 tcttatacca aagagaagag cagaaatagc agcaaatgta ggagaaataa ttcaagaaga
     4741 atttctttct atggacgaaa tacttgcaaa tatcattact gacgaagata aggaagaagt
     4801 tgtaagatac ataaaagcaa gggtaaaaat tataatacat gagaaagttt catttatccc
     4861 gagtggtata aaaaacatga tacaagatta tctaggagag ataattgagt ctgaagttaa
     4921 gcaaagtata gatgaattga gtaaaaatat aataaataaa gcaaatgaac gtatagatat
     4981 acaaaaaatg gtcgaagata agattaatga actagattta tatgaattgg aagagataat
     5041 aataagaata gctaagaaag aattaaaaca tatagagttt ttggggcttg tcttagggtt
     5101 tttaataggt atagctcaag ggttaattac tatgtttatt taaatatatg agaagtagca
     5161 tttatttaag tactaattat atttacctat ttgagtgaat tacttattaa taacatttag
     5221 atattaattg tatttac
//
LOCUS       DAEMHZ010000198         5154 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000198 DAEMHZ010000000
VERSION     DAEMHZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.199"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1553
                     /gene="yfcC"
                     /locus_tag="KPK72_003597"
     CDS             159..1553
                     /gene="yfcC"
                     /locus_tag="KPK72_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG2490765.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            2070..3437
                     /locus_tag="KPK72_003598"
     CDS             2070..3437
                     /locus_tag="KPK72_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG2490766.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            3462..4070
                     /locus_tag="KPK72_003599"
     CDS             3462..4070
                     /locus_tag="KPK72_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG2490767.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            4118..4489
                     /locus_tag="KPK72_003600"
     CDS             4118..4489
                     /locus_tag="KPK72_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG2490768.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            4668..5006
                     /locus_tag="KPK72_003601"
     CDS             4668..5006
                     /locus_tag="KPK72_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG2490769.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNGILEK"
BASE COUNT         2032 a          471 c          901 g         1750 t
ORIGIN      
        1 aataaaaata aaaaagttat cttttaaatt ttaaaaggta gcttttttat tttatatctt
       61 tgctaataaa aaattgtaaa aagggaaaac tttagtcaga gagattaaac aatatcctag
      121 aaagatttaa aatttagtaa agtatggagg acattataat gactgacagt aaaaagaaac
      181 gatttattat accacacact tttacaataa tatttttact aatagtcttg atggcaatat
      241 tgacttggat agtaccaagt ggtgaattta gtagagaaga tgtagaaggt agaatggtag
      301 ttgtaccagg gacttatcaa agtgtagaaa gtaatcctca aggatttgca gatgttttta
      361 aagcaccaat agaaggcttt atcgattcag ctgaagttgt aggttttgtt ttaatagttg
      421 gaggtgcttt tggtattgta aataggactg gagctataga agcaggaata gcttcggtta
      481 tctctaagtt taagggaaaa gaacttctta taattccaat atcaatgatt ttatttggac
      541 ttggaggtac aacatttggg atggcagaag agacattacc attttatatg atatttgttc
      601 cactcatgac atccttggga tatgattctc taacagcaat agcaattgta tttataggag
      661 cagcagcagg agcagccgct tcaacaataa atccattttc agttggtatt gcacaggcac
      721 tagctcaact tgctcctggt tcaggagtaa cttacagagt agttattttt atagcatata
      781 tactaataag tatagcattt gttatgtact atgctaagaa ggtaaaggaa aatcctgaaa
      841 gttcaatagt atatgaaata gataaaaata ataaatcaat acttaatcaa acttcaacag
      901 aaataaaaga atttactttt aaagaagtat ctgttcttgc tatatttgga atagggatgg
      961 caataatgat ttggggtgtt ttaagtcaag gatggtacat tccagaaata tcaatgtgtt
     1021 ttgctgcaat aggtgtgctt tctggattgg ttggtagatt atctcaaatt gaaatctcag
     1081 aaagttttat agatggagca aaagatttag catctgcagc tcttgctata gctttggctc
     1141 gtggaatcgt aataattgct caaaatggat ttataataga tactatatta aattccgcag
     1201 cagcctttct aggtgggctt ccaaaggtta tatttgtata tttatcattt ttcttggaag
     1261 ctgcattggc atttttaatt ccatcatctt caggtcttgc ttcacttaca gtacctgtac
     1321 ttgctcctct tggtgatttg gttaatatat cggctcaagt gatagtaacc gcttatcaat
     1381 ttggtgtggg tcttacaaac tttataactc caacttcagg agtactgatg ggagcacttg
     1441 cagtagccca tattccattt tcaaaatggg taagatttat aatgcctatc ttgttgattt
     1501 tgacagctgt atctctagtc tttttaacaa taggtattta tatagggttt taattaagtg
     1561 tagttttatt taagatattt agtaatggta taaaatgata atatgttttc aataaattgc
     1621 cactaattta attagtggta atttgttttt tgtatttaaa aataaatgta tttacttgta
     1681 ttttgaatta atatctactt agacattgtt ttaattgtta tatttttatt taattaagca
     1741 ttgatttatc attttttata tttcatataa caatgcaaaa aataattttt atatagtaaa
     1801 tgacaaaagt ttacaataaa tgtatatttt cttactcatt ccttaaaatt actcatttag
     1861 tttaataact ctcatttcat atgtaaagtt ggtaaaaaaa ttaaaattaa aagtaaaaaa
     1921 ctaaataaat taatgttaat ttgaaatata tatgaaggtt tttggagaat acttacttat
     1981 gataggaaaa aatgttaaaa taatagtgta atcaaatata aaggactgaa ataattaaca
     2041 gttattaaat attaggagtg taaaaattta tgggattaaa agaatttgat tttattgaag
     2101 agtctattga tatgttgagg acaatgtcac caacattaga aactatatca gatgaaatag
     2161 aagaatattt tgaaaatatc ttggatgaaa agaatcaaga atatataaat gtaacctcta
     2221 gaataaaatc agaatcaagt ttaagagaaa agataattag aaatagatat ttaaaaaaat
     2281 atggagaagc aagtaatctt attcataatg tttctgactt aataggccta agaattgagt
     2341 gtagatttat tgaggatgaa aacaaaatat atagactttt gagaagatat tttaataaaa
     2401 cagatgacaa aataaactat tataataaag agaataagaa tattaaatta aaactatctg
     2461 agaggcaacc caataagcaa aaaaatggat ttgaaatata taaaatagat ggagtttttg
     2521 cgtatttaga tagagaagta aaatttgaac ttcaaattaa atctcttgta aatgtgtttt
     2581 ggagtgaaat agaacataaa ataatttata aaaacaatac atatttactt gctgataaat
     2641 ttataaagga tatgatggac tctataaaga ataatcttac tatgatagat aatcagcttt
     2701 taagtattta taaaaacttt cattcaggga agtcttttaa tatgaaagta agtaaaaaag
     2761 aaattgaaaa attatttgcg aaattagtct atgacgcttt ttctgaaaag atgaataaaa
     2821 gtataggttt tgttgtagat tttaaaaaac catgtgagac tatattgaac tattctttta
     2881 ataaacaaga tataagtgat gaagttttag gtaattttat gcttgatgag tttgcaagat
     2941 taaatgaaat tgtaaacaag gatatagatt ttaatgagca aatagaattt gaaagagaac
     3001 caacatttga tgataagttt tgtaaagacc taggaaatca ttttagaagt agattaaaca
     3061 cagaatttcc atggaaccta ttttttagaa tattatttga aatagagcca tataacaata
     3121 ccaaagactt tgaaaacttt gtagagttta taaaagaaaa tgtgctacta gaagaaaata
     3181 gggagcaatt attatgccaa tttgaagaag atagcaaact tataattgaa gatatatatg
     3241 attgtatatt taatagtata agtgagattg atagtgttga aattttatat aactataatc
     3301 ttgaaaaaat aaactttaca tcaagtgaaa tcataaattg tatatgtaga gaatatgagt
     3361 gttatggtga atacttagaa gaaaaagaaa agcttatgaa tacatttaaa gaaaagatta
     3421 ttcaaatctt tgagtaatag gtcaatgctg aagtggcgct aatgcgccat ttttcaaata
     3481 tatatgaatt aagtaaaatt attttggagg aggcgatttg tatagagaaa acaggtgtaa
     3541 ttttggcagg gggaagaaat tctagaatgg gtagagataa agccttttta gagttacatg
     3601 ataagctttt tattgaaatt gcaatagaag catttaaaaa ctttgatgag ttgataataa
     3661 tatctaataa tgaagaatta tattctaaat acgatattaa agtttataat gatatagtaa
     3721 aagatgttgg tccaattgga ggaatatata ctgctcttga atatgcaaag tatgatatag
     3781 ttacaattgc atgtgatatg ccatatctaa ataatcaaat agtcgaaaga atagctaata
     3841 aaatgaatga taaaagtgtg atttcagtga caaatggaaa gttacaacca ttgtgttctg
     3901 gatataaaaa gagtataata aataaagtat ctttatgtat aagggaaaat gatttaaagc
     3961 tcagaagctt tatagataaa atagataaaa gttatatata ctttgatgaa gaagatttat
     4021 ttttaaatgt aaacactgtt gatgagtatg aaaaattaac taaagtttaa ttagctatgg
     4081 ttgtaaaata gagtaggatt tatatggagg agagtttatg aatttactta aatgcgattc
     4141 ttgggcagta atattaaata atagcgataa agaaagtaaa gcatataaaa tattagatga
     4201 acttaaaagg aatatgtaca aagttgttgc tatagatgaa gagaaaaaac ctattgaagg
     4261 aatagatgtg tatgaatgtt taaaagatgt tccacacaat attgatgttg tagctattat
     4321 tgataaacaa tctaagatgg atgtgatttt agaagaagta gagcttttgg atatacaaaa
     4381 tatgtggttt gaaaaaggaa gctttagtga aatgatgatt aaaaagacta aagatttaaa
     4441 attaaatata gaatataatt taagtttata tgatgagttg actagataaa attatatatc
     4501 ttaataataa aaatataatt agttaataaa acatattatt ttatagtata gattggttaa
     4561 taaaagtaca gattggttaa tgaaacatta aattacaata tttgttaaaa aaattgtgag
     4621 ttacatgata taatgtatta aataggatac taaggagttg atatattatg ttaatagtaa
     4681 ctacagaaaa agtagaaggt aaaaagatat caaaggtttt aggattagtg agaggaagta
     4741 caataagagc aaaacatgtt ggaaaagata taggagcaag ttttaagaat cttgttggag
     4801 gagaacttac tggatataat gaaatgctca ctgaagcaag acaaattgcc ataggtagaa
     4861 tggttgaaga tgcagaagct aaaggtgcaa acgcagtaat agcatttaga ctgtcttcag
     4921 cttcagttat gcaaggggca gcagaaatgc ttgcttatgg aacagcagtt gttttagaag
     4981 atgataatgg tattcttgaa aaataaaata taaatagtta aaaagaggca tcttttaaaa
     5041 attttatgga tgcctttttt tatttgaaaa aactaatttg agaagaaatt ttaactatta
     5101 tgacaataaa attagggtga tattatataa ataagtaaaa ttatattata taat
//
LOCUS       DAEMHZ010000199         5099 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000199 DAEMHZ010000000
VERSION     DAEMHZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.200"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            706..1737
                     /locus_tag="KPK72_003602"
     CDS             706..1737
                     /locus_tag="KPK72_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2490770.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1870..3168
                     /locus_tag="KPK72_003603"
     CDS             1870..3168
                     /locus_tag="KPK72_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG2490771.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3282..3527)
                     /locus_tag="KPK72_003604"
     CDS             complement(3282..3527)
                     /locus_tag="KPK72_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2490772.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3675..4541
                     /locus_tag="KPK72_003605"
     CDS             3675..4541
                     /locus_tag="KPK72_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG2490773.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4545..5081)
                     /locus_tag="KPK72_003606"
     CDS             complement(4545..5081)
                     /locus_tag="KPK72_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG2490774.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
BASE COUNT         2057 a          514 c          805 g         1723 t
ORIGIN      
        1 attaatattt aatggagtac aatataaata ttaaagcgac atcttatagt ttattgaacc
       61 tatagtaatg aattaataaa tagtttaatt ctatgaaaag atttcctttt atgcaattta
      121 tttattgaaa tatgatataa tatataaagg tgtaagtatt agttgttaaa ataatattta
      181 tattagtttt tatgcatact tggtataagt ttttcatata ttgattaaaa ttgatgttga
      241 tttattattt ttagtatttt ttcacctttt gattcatact ttgtagaatg gtgtattttc
      301 taaaactgat tataagacaa gttcattcaa attagcattt ttatatcata taaataattg
      361 ttccccctaa ataagattta gatgtatagt attaaaatca aaaagtataa aataaaattg
      421 atgcttaatc atagataaat attaataaaa tatacaaggg gattgtctaa taaaaagata
      481 atctcctttt ttacaaataa ttctaaattg tgatataaat ctaaaaattt tatcttaaca
      541 tttaaccatt taatgataat aattgatata ataaatataa attaaatata tggtgtactt
      601 attttatgtt tttagcaaga aatatagcat ttaaaagata aattgtgcaa cttaaatcaa
      661 agtttaagat gaactattaa catagatata ttaaaaactt ttgtaatgga gaatatatgt
      721 gatatgaata ataaagttaa ggttgcagta ttagatacag gaatagacaa agaacatgat
      781 tacttaaaag ataatttagt tggaggaatt gcttttgaat gtatgcatga ttatatcttt
      841 atatcagata aatttgatga tgaagatggt catggaactg cttgtgcatc aataataaag
      901 aaagagtatg aagatgtaga gttatttgta ataaaaattt tgagaaaaga tggtattaca
      961 aatataaaag ttcttgaaga agcattaaga taccttttag acactaacat aagattaatt
     1021 aatttaagtt tatcagtcat aggagtagaa agtgttaagg gcttatttga agtttgctat
     1081 gaattgttta gaaaaggaaa aataatagta tgttcactcg ctaatgattt tgacttaagt
     1141 tatccagcta tgtttaacaa tgttataggt gtaagaggat ttactttaga tggagatgat
     1201 tcattttggt acaacaaaaa atatgatgtt caatgtgtta tggatagcaa ttcatatata
     1261 agttgtgata taaataattc ctatagatta ccaccaaaat gtaatagtta tttatcagca
     1321 aagcttactg gtaaaatagc taagatatta tcagaaaaac caggcataac aatatctgat
     1381 ttaaatgata agcttgaatc attagcaact agaaatcatt gggatagttg tgattttgat
     1441 gagtgtagta gaattgtaaa ctttaaattg gatttgtatg acaaagaaaa tgctcttctt
     1501 atgaaagttg ctgacgttgt tagggattgc ttagatattg aagataataa tgaaaaactc
     1561 tttaagggta gcctttttaa taaggagata ggcttggttt atgataattg ttttaatctg
     1621 ttgaaaaagc tagagcatag atttgacata aagtttaatt atatggacat ctcaaaatat
     1681 gacttagttt caatatatac actgactgaa ttagtggaaa gatataaaaa cttataaata
     1741 aagatttgta gctaaattta atgtagaagg tattaaatta tatgtattct ttgtcggtag
     1801 attaaaagta aaaaatatac tataattaga tagcataaga gaaaatgcca atacaggggg
     1861 agttagataa tgaaatatga agaagcatta gagtacatat cacaaactaa taaattcggt
     1921 ataagattag gattagaaaa tatcggaaag ttattagaat tattaggaaa tccacaagaa
     1981 actttgaata taatacatgt agctggaact aatggaaaag gttctgtgtg ttcttttgtt
     2041 tctaatatac taagagagtg tggatataaa gttggtttat atacatctcc atatttagaa
     2101 acttttactg agcgtataag agtaaatgga caaaacatac cacaggatga tgttgctaga
     2161 ataattgagt taataaaaga aaaaatagag attatggtaa aagaaggcta tgcatatcca
     2221 acagaatttg aagtggttac tgcaatggca ttttattact attcagaaca aaaggtagac
     2281 tttgtggcat tagaagttgg tttaggtggc agatatgatg ctacaaatat aataacaaaa
     2341 tcacttgtaa gtgtgatagt atctataagt ttggaccata caggtatact tggagataca
     2401 atagaaaaaa tagcgtacga aaaggctgga ataataaagg aaaatggagt agtactagta
     2461 tatgaccaga ctgatgaagc aaaggatgtt ataaagtcag tttgtaaaga gaaaaaagct
     2521 aaatatatag aagttgattt tgatgatata aacataaaaa aatcagatat aaattctcaa
     2581 atatacgatt gtactgtaat gaaagaaact tatagagacc ttgaaataaa attgataggc
     2641 gaacatcaaa taaacaattc tatcttggca attagtgtaa taaaatatct aaaagacata
     2701 aataaattag caaatataaa tgaagaatct ataagaaaag gacttataaa tactaaatgg
     2761 ccaggaagaa tagaaaagat aaaagaaaat ccaattttta taatagatgg tgcacataat
     2821 gaagatggag caaaatcttt ggcaaaagca cttgataaga attttaaagg tagaaaatta
     2881 accttattaa taggtatgtt agaagataaa gatattgata gtgttttaga gatattgttg
     2941 ccacatttta ataaggtaat aacaacaaca ccaaataacc caagggccat aaattcagat
     3001 atattaagag aaaaagtatt aaaatatgta gatgatgtaa catcaaagca tgagatagag
     3061 gatgcagtaa attatacatt agaaacatca agtgaggatg atattataat aagtgcaggt
     3121 tcattatata tgattggtac agtgagaact ttagtaaaga aattataatt ttataaaata
     3181 agtatacaaa taaaaatata aaaaagagtt gtctaaaaaa tggacaactc tttttatata
     3241 aaataagttt ttaataattt tactgatgtt tatataagta attatataga ttgacgtata
     3301 gctttagata attggtctgg gcaagaagta ccttttccac cacagtcaat accatttaat
     3361 ttatcagcaa cttcaagagc attttggcca attattaagc ttctaagtcc tgataaatta
     3421 ccattacatc ctcctataaa ttcagcatca acaatcacat tattttcatc aacttcaaaa
     3481 atcatttctc tacaacatac accacttgga ttaaatgtta ctttcattaa aaagacctcc
     3541 attaaatatt atttcttttt ttaacattaa taataatacc aaatatatgc gtaataatgc
     3601 aactataaag cataatacaa gctaatgaaa taatatatta ttgtagtata tgtattgtag
     3661 agaggagggg ttatatgaaa atctatattt ctaactactt atcaaagagt attagcatag
     3721 ttgattattc tacacttgaa ctagaaaaag aaatagtatt agaagataat atatatccac
     3781 accatttttg catagaaaaa gaaaaaaatt taatatacat acctagttca agtaatggga
     3841 tactctatgt cttagattta agtaatgata aaataattga tactgtctct ataggaggaa
     3901 gtcttagtca agttgtactt agcgataatg aattgtttgt ggcaaatgaa gattctaata
     3961 gtatatatgt actagataaa aacacattaa atccaatagg aattattgga gttgataata
     4021 tgccacatgg atttgacttt gatagagaaa gcaaaaagtt atatgtaccg tgtataaact
     4081 cgatagtatg tatagataca attaataaaa atatacaaaa gaaaataaat atggacttta
     4141 gagcttggca tatagaactt gataaacaaa agaaagagat gtatatatca acacttgatg
     4201 gaaagcttat aataatagat gaagtaagca tggatataaa aaaggtatta tatgaatttt
     4261 tattaccagt agaaataaga tttaattata gtggcaaaaa agtttatgtt gctgatttgg
     4321 gttataataa tgtaagaatt ttagattata caacgagtag atacattgga aatattgaaa
     4381 taaatgggat tccccaagga ttagaaatat ctaaagatga aaagttacta tttgtatcag
     4441 atacacaaga aaattcagta aaagtttatg aaactgcaaa taacaaatta ataaaagaaa
     4501 tcaaggtagg aaaagagcct acaactataa tttgtttgta ggctttaatg tatgtctctt
     4561 ataagtaaat cgataacttc ggcatacata taagatgcat tatttttcca gttattgcaa
     4621 ataagttttg cttgtttctc tgatgaaaca tttagattta aatctattaa gttagactga
     4681 ttttctatga ctctaaggtt gacaataaat tcattatcac tttgttttac aaaactagac
     4741 tttacttgag tatctgctag tagattttct ttgttagaat ctatatactt gtctattttt
     4801 tcagtaacac tagtaggtat tcgattaaag aagtattcta gactttctac gcctctttga
     4861 gtaagagcat aatattctct gtcagagtct ttatatgtag tcaaaaactt agattccata
     4921 agctgagata aaagctgttg taatgaaaaa taattcatca tctcagtctc taatactact
     4981 tgagttattt gagaattagt taaatccatt ttaatttttt ctaatatata taaaattaaa
     5041 agtttgtgat aggctaattc ttcagacgag ttttcaaaca taaatatcat cctccgtca
//
LOCUS       DAEMHZ010000200         5076 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000200 DAEMHZ010000000
VERSION     DAEMHZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.201"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1028)
                     /locus_tag="KPK72_003607"
     CDS             complement(<1..1028)
                     /locus_tag="KPK72_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG2490775.1"
                     /translation="MSDDLVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGAPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNT"
     gene            complement(1021..1722)
                     /locus_tag="KPK72_003608"
     CDS             complement(1021..1722)
                     /locus_tag="KPK72_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2490776.1"
                     /translation="MAYDFYLDGVQLPIPPGKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLRLV"
     gene            complement(1734..4085)
                     /locus_tag="KPK72_003609"
     CDS             complement(1734..4085)
                     /locus_tag="KPK72_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG2490777.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLRNPLN
                     TGSIQASQQSLNNIESILIRIEQKIKGNTNEQENFNNKIRQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVVKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFKSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFE
                     SMPITFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSSFVQGITLGAVLIIQGLGGITNAALSVVNIFAQNWSIIAPIVYG
                     VIAAISLYSIALAGIWIWEKLVAASKFAMMFAQMLLNTKTLSGYAYLIKYAAGQWIAN
                     EAMLACPIFWIVAGILAFIVVVFVAVAAINKFAGTSLTALGVIVGAVFAAVAAIQNVM
                     IWLLNGCITVNEGIINGWNQCVYLMKQAIAKGVIFIIEKMASLNDSVNNAGNALGKAF
                     IDGANIAIRGVNKLIDLLNKIPGVDIGKVGEATFTPVKADNSYIKQQIDSLNKWVGNA
                     PEKVKLERMGYKDIGAEFEKGNALGTKWQKSLEKKLKDTFDINKMLEDAKDKLGLKDL
                     WDKNNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINRFT
                     GVNIKIDMNNTNNISKDADVDGIVNILTEKLNDAMVVSAEGVV"
     gene            complement(4157..5023)
                     /locus_tag="KPK72_003610"
     CDS             complement(4157..5023)
                     /locus_tag="KPK72_003610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490778.1"
                     /translation="MWQRFKKLNLFLKIIIGFIVLLFFPVFLILLIAEFTVKSFKNKK
                     IVRGLIGIFIIFIFAGEVKVYLAQAWGINIGSQVRVSNESEVNRNNELESKQDDKKVV
                     NMSEDEQKISKVTNLAPEQVQNIAKILKECGIEEFESIKHDEGLDNAHFDGEKGYRIE
                     FGGSKNIILYMNKDNSVYNVRWADNDFYKDGKVVSKVSDYTLTMDEKTALQLQCQNGV
                     SAILKSPSTAKFPNITKWMFSKNKEEIVVQSYVDSQNSFGATMRSEFQIIFTSDGKDV
                     KSFIFDGKEMIK"
BASE COUNT         1480 a         1006 c          599 g         1991 t
ORIGIN      
        1 gtatttccac tactatatga gcttgaagaa taagaagcaa attcatctcc atcaacaagg
       61 gtcaaatcca taaagtgcga attattttca aatgtatgtt ttactttctc aactaacata
      121 taattttgta attcaatatc tcctagattt aaaaaaacag gtactaaaca acctgctctt
      181 actctaatat caccaagtgc attttttaaa cttaatgact tagttttctt attatatagt
      241 tttagaagta tatcacactt ttgttttatc tctgcttcac tcatgttttt gtctactgta
      301 tcaaacattt gaagtattcc ccaacttctc atatgcgtag aatcttgtgc aatatacaca
      361 tcccttttac ctgtctcttc gttgtctctt actagtttta tttttgtata agtatcacta
      421 tctattgatg aattatagtc aaaatcctca attacatcat tgttcatgac agtatccagt
      481 ttcattgatg caacattctt taatgttatt cttccaaaat catcatacaa ggtatacatt
      541 tcttttttct ctcttaaagt atcatcaagt gcagttagta tcatatcaaa taaagtttta
      601 ttctcctcta tcctagagat tttatactta gtgtcttcta tgacattgta ttttaaatta
      661 aaatctttag ctaacatttt tacaagttca cttgcagttt tattactata tacgtaagta
      721 tctttatttt taaaatatct taactggtcg taagcaacaa ttttaatgtg attttcttta
      781 tctcttttct tctgaaatat atatccataa aatataccta ttcctttata atacagcctt
      841 acagagttac cttcacagaa ttgtagtata tcatccatga ctattgtaaa ttctaattta
      901 cttggcgctc ctcgtctttc tatctcccat gtgattccat ctaaaacgac aggttcatag
      961 aaatcttccc aatgtgctat gactaatctt acatctctat catttgctaa cactaaatca
     1021 tcagacaagc ctcaacacct gccctttgta aatagtatat ttacttaaat tcttcccctt
     1081 atttgcctta tccatcattg atttatttag ttcatatact ttcttatata gtgaaccatt
     1141 tcctaattgc ttttgacaaa ttgcccaaag agaatcccct gccttgactg tatatgtttt
     1201 accatttggc ttattggatg aatctggtct aaactctttt ggtttcatga ctggaggggg
     1261 agtcctgcca tagtttgtct tttcaggtgt tgcaagtact aactttttag ttgaataatc
     1321 tctatattgt tttaacttta ttgcaacttt tgtatctgag ccattatctg catcttctga
     1381 aatagcatac tcttcaagag atactttcat attagtatta aataatactt tgttacctaa
     1441 ctcacgggac acaatgaatt gaaatggctt acaatcagtt tttagtagtt ccagtttact
     1501 taaaaagaat tgaacatccc taaaagttcc acgataaaaa ggtaatttat tatgtgtaaa
     1561 ttctgcttca aaacttattt cagatagccc ttcttttttt aatatattta cttctccaac
     1621 atttattaaa tcaactgtct tatttttatt tgtcactttg acttcaagct taccaggcgg
     1681 gattggtaat tgtactccat ctaaataaaa gtcataagcc atttatatcc ctcctaaact
     1741 actccctcag cagatacaac catagcgtca tttaattttt cagttaaaat attgactata
     1801 ccatccacat ctgcatcttt acttatgttg tttgtattgt tcatgtcaat tttaatgttt
     1861 actcctgtga atcggtttat tacctcctgc tctgcaatat ctctaagata ttttaaatct
     1921 tcttgacttt tatccattgt tttagccatt tttgcagtgt ttcctgccgt gtcctttaca
     1981 ttttttccta aatctccacc aaatcctcca agattattaa gtggattgtt tttatcccac
     2041 aaatctttta atcctagctt gtcttttgca tcttctagca tcttattaat gtcaaaagta
     2101 tcttttaatt tcttttctag agatttttgc cacttagttc ctagagcatt tcctttctca
     2161 aattctgctc caatatcttt gtatcccatt cgctccaatt taactttttc tggtgcattt
     2221 cctacccatt tatttaagct gtcaatctgt tgcttgatgt aactgttgtc cgccttaaca
     2281 ggtgtaaatg ttgcttctcc aactttccca atgtccactc caggaatttt gtttagtaaa
     2341 tctattaatt tattcacacc tcgtattgct atgttcgccc cgtctatgaa tgctttccca
     2401 agcgcatttc ctgcattatt tacagaatca tttaatgatg ccattttctc aattataaag
     2461 attacacctt ttgcaatagc ttgtttcata agatatacac attgattcca accatttata
     2521 attccttcgt ttacagttat gcaaccattt aataaccaaa tcataacatt ttgtattgct
     2581 gccactgctg caaatacagc acctacaatt actcctaagg cggttaaact tgttcctgcg
     2641 aatttgttta tcgctgctac tgccacaaaa actactacta taaatgctag aatacctgca
     2701 actatccaaa aaattgggca tgccagcata gcttcatttg ctatccattg cccagctgca
     2761 tattttataa gatatgcata tccagataaa gtctttgtat ttaaaagcat ttgagcaaac
     2821 atcatagcaa atttagatgc tgcaactagc ttttcccata tccaaattcc tgctaaagct
     2881 atagaatata atgaaattgc tgctattact ccataaacaa taggtgcgat aattgaccaa
     2941 ttctgagcaa atatattaac aacactaagt gcagcattag taatccctcc gagtccttgt
     3001 attattaata ccgctcctaa tgtaattcct tgtacaaaac ttgaaaagaa atcactgttt
     3061 aatatatttt ttataatatt taatgtatca taaatatttg aagatattgc aatcattaca
     3121 tttcgcatag atacgacaat ctcactaaat ctaacacttt gtaatgctcc acttatcttt
     3181 ccgagggttt gcccaaaaat catgtaggca tcatttttca tcatagtaag tgcttgacta
     3241 aaagttattg gcatactttc gaatttcttt tctatctcat cagaagcttt aaacagtgca
     3301 tttcttataa catctgctgt tattgctcca ctacttgata attccttcaa ttggtcttta
     3361 gtttttccca ttgcatcagc tattttagta gctagaagtg gagcattttc cataattgac
     3421 ttaaactcat caccttgaag ttttcctgca cccatagctt gagtaagctg atacatagcc
     3481 gcactagctt catttgctga tgttccacca attacaaaag ctttattcat gagttctgta
     3541 aactttacca cctctgctga acttccaaaa gcatctcctg caagtattcc aagtttagca
     3601 acctgtgctg ctgtatctgc ataactagct cttgcacttt gggctgacaa ataaatcatt
     3661 ttattaagct ggtctgttgt ctgtaagcca tcattcatta gatttaaacg tgcttttgta
     3721 cttgcaattg tatctgctgc ttttgtaata ctttctatac ctcttattcc aatatatatg
     3781 ccagccaaac tttttaattt agacactaat aaagaacctg cttcacttcc ctgtcttatt
     3841 ttattattaa agttttcttg ttcatttgta ttccctttaa ttttttgttc tatccttatg
     3901 agaatacttt ctatgttatt caaactttgt tgagatgctt gtatactacc tgtatttaag
     3961 ggattgcgca acctttgttg taacctctcc aagctattaa ttgttgtgtt aatggatgta
     4021 gtcatattgc gaaatgcagg tgtcattccg tcgaaaatct ttattgaagt ttgtattgta
     4081 gccatttcct cactctcctt tcttaaatct aagtacaaaa aaagagtaac caatttgatt
     4141 actccttaag ttatttttat tttatcattt cttttccatc aaatataaat gatttaacat
     4201 ctttgccatc acttgtgaat attatttgaa actcgcttct cattgttgcc ccaaatgaat
     4261 tttgagagtc cacataagat tgaactacta tctcctcttt atttttagag aacatccact
     4321 ttgttatatt cggaaatttc gctgtacttg gtgactttag aattgcacta acaccatttt
     4381 ggcattgtaa ttgcaatgct gttttttcat ccattgtaag agtataatca ctcactttag
     4441 aaactacttt accatcttta taaaagtcat tgtcagccca tctgacattg taaactgaat
     4501 tgtctttatt catatataat attatatttt tagaaccgcc aaactcaatt ctataacctt
     4561 tttctccatc aaaatgtgca ttatccaatc cttcatcatg ttttatgctt tcaaactctt
     4621 ctattccaca ttcctttagt attttagcga tattttgaac ttgctctggg gctaaatttg
     4681 taactttaga tattttttgt tcatcttctg acatattcac tactttttta tcatcttgtt
     4741 ttgattctag ttcattattt ctgtttactt ctgattcatt acttactctt acttgtgacc
     4801 caatatttat tccccacgct tgtgctagat aaacttttac ttctcccgca aatataaaaa
     4861 ttataaatat tccaattaag cctcttacta ttttcttatt tttaaaggat ttaacagtaa
     4921 actctgcaat aagaagtatt aaaaatactg gaaaaaataa taatacaata aaaccaatta
     4981 taattttcag aaaaagattt aattttttaa aacgctgcca cataatataa catcccccta
     5041 taacattatt taccaatatt atactatata agtaaa
//
LOCUS       DAEMHZ010000201         5056 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000201 DAEMHZ010000000
VERSION     DAEMHZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.202"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            546..1055
                     /locus_tag="KPK72_003611"
     CDS             546..1055
                     /locus_tag="KPK72_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG2490779.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1083..1277
                     /gene="rpmI"
                     /locus_tag="KPK72_003612"
     CDS             1083..1277
                     /gene="rpmI"
                     /locus_tag="KPK72_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG2490780.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1333..1689
                     /gene="rplT"
                     /locus_tag="KPK72_003613"
     CDS             1333..1689
                     /gene="rplT"
                     /locus_tag="KPK72_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG2490781.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1999..2544
                     /locus_tag="KPK72_003614"
     CDS             1999..2544
                     /locus_tag="KPK72_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490782.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2704..2880
                     /locus_tag="KPK72_003615"
     CDS             2704..2880
                     /locus_tag="KPK72_003615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490783.1"
                     /translation="MPKEMNFRPHMQLGCIIQLDQIYKIKISIFIADKLTVILLKNHL
                     LVIVSILKEIIVIY"
     gene            2980..4905
                     /locus_tag="KPK72_003616"
     CDS             2980..4905
                     /locus_tag="KPK72_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2490784.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
BASE COUNT         2144 a          491 c          947 g         1474 t
ORIGIN      
        1 tgtattgatt aagtattgaa taaaatatta caaatttagt tgaaatgaaa atgataaaaa
       61 atcttgtact atattttaca aatcatataa atagagaaat tgttgtagca caaagtagta
      121 ggtagtatat tagaatacaa tatagaattt gttgtgtagt atagtgttat attagaatac
      181 aatataggat ttgttatgta gaatatatag agtcatgtag aatataaata tataaaagga
      241 ttttgggtat aaatttaaaa attaatagca aaatgagtag tatagacata taatgagtag
      301 taggaagtaa ttaaattttt cctaaacaaa aaaatagttg caaataattt gattaagtga
      361 tatagtaaat aaggtaagaa taaaacaaag aagaagtccg cttctcacct tacgccggag
      421 gttagtaagg taaatataag ttaaagctta attttaattt aagtttgttt aaatttggta
      481 acttatattg cggatgatta cgtcatccgc tttttatttt ataaaaattt tggaggtgtc
      541 ctacaattag caaagaatta gcaatcaatg aacaaataaa agataaggaa ataagagtcc
      601 tttcaccaac aggtgaacaa cttggagtaa tgcctactaa agaagctcaa gcaatggcta
      661 atagcaaaaa cttagatttg gttcaaatct cgccgaatgc aaatccacca gtatgtaaga
      721 taatggatta tggaaagttt agatatgagc aagcgagaaa agagaaagaa gctaagaaaa
      781 aacaaaaaac tatagttgta aaagaagtaa gacttagacc aggtattgaa caaaatgatt
      841 taaatacgaa agctaataat gctataaagt tccttaaaaa aggggataaa gtcaaagttg
      901 aacttagatt tagaggaaga gaattaggac ataaagacat aggtaaagaa gttatgctta
      961 agtttttaga tataataaaa gagtttggag aaccaacaaa agcccctgca tttgagggta
     1021 ataatatggt tgtaattata gatccaaaaa aatagatgat taactgagag gaggaatttg
     1081 tcatgcctaa aatgaaaact catagaggtg cagcaaagag attaaagaaa acaggaactg
     1141 gaaagttaaa gagagctaaa gcttttaaaa aacatatatt aactaaaaaa tctgcaaaaa
     1201 ctaaaatgaa cttaagaaaa tcaactttag taagtgatgg agatgctaag agaatagcac
     1261 aattattacc atactaagat acagattgta aataataact atagaagagg gatataaaag
     1321 gaggtcataa cgatggcaag agttaaaaaa gctatgaacg ctcgtaaaaa gcataagaaa
     1381 atattaaaac ttgcaaaagg attcagagga tctagaagca aattatatag accagcaaat
     1441 acattcgtaa tgaaagcatt aaaaaatgct tatataggaa gaaagttaaa gaagagagac
     1501 ttcagaaaac tttggataca aagaataaat gcagctgcta gaatgaatgg aatatcttat
     1561 tcaagattaa tgaatggatt aaaattatct ggtattgaag ttaacagaaa aatgttatct
     1621 gaaatggcta tacaagaccc agaaggattt gcaaaattag cagaagttgc taaagcaaaa
     1681 ttagcttagt attgaatcat gagatgtaaa gtctttgagt tatataaata attcaaggac
     1741 ttttttattt tttgaaaaat acttattagt gtcatgtaca actaatttta taatgataaa
     1801 taaaataagc aatacaatag aattttatga gatttattat aagaattgtt acaattattt
     1861 atgatagcta taaaaagtca tagtaaaaat gatattgatg tatgttgata tatgtataaa
     1921 gctttcaatc aatcttgaat taatattatc aacatgataa tattatctta ttaataatat
     1981 aaataagggg gaaataatat gaatgaaaaa ctaatggatt gttttgtaaa cccaattaaa
     2041 tgcaagttat tgattgaaat tttttcaaat aatcgaacta cagctaaaca attaggagaa
     2101 aaatttcaag atatatcaaa ggcaacactt tatcgttacc ttaataaaat gttgaaagat
     2161 gatattataa aagtggttga agaaaataaa ataaggggaa ctgttgaaaa agtatatgct
     2221 atgaagttag atatagctga tgatgtgaac aaaattatag atgagaactc tggagaagaa
     2281 tatgctcaac tgtttactca atatatgatg ggatttttaa gagaatttta tgaatacgct
     2341 gaaagaaagg aaaaggatat cctaaaagat ggttcaggat tttctgtctg tccagtatat
     2401 gctagtattg atgagatagt tgaagtgagt aaaaaaattg ctagtatact agaacctcta
     2461 agaaataact cacatgaaga aggtagaaga atgcattcta taggagtgat aattactcca
     2521 cctaaaaagg atgaagggaa ataaatctga tttacatggg gtagagccaa tttatacagg
     2581 gaatcgtttt atgatttaca tatgtatcca gagcaaagta tctctgcttg gattgtactt
     2641 cattgtacca tttcatccaa tgacttggag tggtacaatg aagtttttta atttttcata
     2701 ttaatgccta aagaaatgaa ctttagacca catatgcaat taggttgcat tatacaatta
     2761 gaccagatat acaaaataaa aatcagtatt tttatagcag ataaattgac tgttattctt
     2821 cttaaaaatc atctattagt tattgttagc atattgaaag aaattatagt aatctattga
     2881 ataaatgaat attgcccata atcatataaa ctgctatagt ttaaaataaa gcaggacgtt
     2941 aaattttata gtaatctaaa aatttaagag ggggatttta tgaaaataaa atttgttatt
     3001 atgccattgg tgatattttt aatggtatta tttgtggggt gtcaaagtac aaaagataca
     3061 gaagttaaag caaaagatac agaaattaaa atattaggga caggtgattt acattcaatt
     3121 ttgacagact ctatggtttc atatgtaaat gaggaaaggg aaaagaataa aaatctgctt
     3181 atggtagatg cgggagattt ctatggtaca caaagtagag aaatgtggga atggtcttct
     3241 ggaaaaaagt taattaatat taaaagaaat ggtagagcag agtatgatga cataattaag
     3301 agtagtaaag atgaagtacc tatagttaaa gatatggcaa agttgaaata tgatgctgta
     3361 gttttgggag ataatgaatt tgtttcaaat gataagcaaa gtcttgataa actggtttct
     3421 gattttaaaa ataataatat gccattggta tcagcaaata tatatgaaca atctggagaa
     3481 aattatgtac aaccatatgt aatgaaaaat attaagacag atgagggcaa tgtaaaagtt
     3541 ggaatactag gtcttactat aaaagaagta ggggaaaccc ttggtcttga ggattttgaa
     3601 aaggataaaa aagctagaga gcttggagaa caagctgatt acaaaggaaa attatatgca
     3661 aatgatttag tagaagatgc aaagaaatgg ataaaagtta tggaaaaaga aagtccagac
     3721 ataatagttg cagttgtaca ctcaggggaa gaacctaaaa cagatagaaa tccaggaaat
     3781 agaataaaag agttagcaac tacagtggat ggaatagatg caattgtagc tggtcatact
     3841 catgaaaaaa tagatgaaca caaatataaa aataaatctg gagatgaagt aattgttaca
     3901 caaccaggtt ggcatggaga tagagtatca gagattaatt tcaaattaaa taaagagaat
     3961 ggaaagtggg acataaagga aaagtctagt aaattaaaaa taattgataa agaagtaagc
     4021 atagttgcta ctgcagattt acattcacat ttctctgata aattaacagt tgaccttgtt
     4081 aatgaacgta gtaacccaat tgaacctgtt gtagtagatg caggcgactt cttagaccct
     4141 caaacagatg aaatgactca atggtataaa gagtggaaaa gtataagaga taataatagt
     4201 gataaaacga ttagtagatg cccaatggtc atggatatga atcagggaat gtatgatgca
     4261 gtagtacttg gaaatcatga atttgtatct gaaaaagatg aattttggtc agatgaacgc
     4321 cttttagatg ctgttataga ggattttgaa caggtggcaa tttctgtgtt atcggcaaat
     4381 atatataggc aatctggtaa aaattatgtt aaaccatata ttataaaaga tgtggagacg
     4441 agtgaaggta atgtaaaagt aggaatattg ggacttacta taaaagaagt aggtaaaggt
     4501 cttaaaaatg taaacttaca agagcaattt caatataagg ataaattgta tgcaaatgat
     4561 ttagtggaag atgcaaagaa atgggtaaaa gttatgaaaa agaaaaaacc agatataata
     4621 gttgcagttg tacattcagg agaagaacca aaaaatccaa aacatccagg aaatagaata
     4681 aaagaactag caactacagt agatggaata gatgcaattg ttgctagtca tactcatgaa
     4741 aaaatagatg agcatgaata taaaaataag tctggagaaa aagttatagt gacacaacca
     4801 ggtgagcatg gtaaatatta ttctaaaatt acctttaaag taaataaaga aaagggttcg
     4861 tggattatta atgataaatt cagtaaaaca attgaggttg aataacttta taaaatttat
     4921 agataaagta aattatctga tattgacatg atatataaga taaagtttct aatattagta
     4981 taatgcataa tgtgaaatat tttaataata agagaataaa caagacaaat aaaaatattt
     5041 taacatgtaa gatata
//
LOCUS       DAEMHZ010000202         4914 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000202 DAEMHZ010000000
VERSION     DAEMHZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.203"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..928
                     /locus_tag="KPK72_003617"
     CDS             2..928
                     /locus_tag="KPK72_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2490785.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            1067..1213
                     /locus_tag="KPK72_003618"
     CDS             1067..1213
                     /locus_tag="KPK72_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490786.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1318..1842)
                     /locus_tag="KPK72_003619"
     CDS             complement(1318..1842)
                     /locus_tag="KPK72_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490787.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(1895..2119)
                     /locus_tag="KPK72_003620"
     CDS             complement(1895..2119)
                     /locus_tag="KPK72_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490788.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            2316..3440
                     /locus_tag="KPK72_003621"
     CDS             2316..3440
                     /locus_tag="KPK72_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG2490789.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCYTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(3914..4738)
                     /locus_tag="KPK72_003622"
     CDS             complement(3914..4738)
                     /locus_tag="KPK72_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490790.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         1654 a          744 c          533 g         1983 t
ORIGIN      
        1 tatgaatttc agcaatattt ttatccaagt tgcagtttta tttataatta ttttagttgg
       61 atattttgtc agaaaattca acttattaga tgaccactgc acatctaagc tttcaacttt
      121 gacaatgaca gtttttttac catcaatgat aattagctcc atgcaaatta actttgatag
      181 taaaatgata caaaaaatac tattgttatt atttacatct ttgattatgt atatagtttc
      241 aattataatt gcatttttat tgaaatacat tttaaaatgt aatgataaaa aagatttggg
      301 aatatatcag tatatagttg ttttttcaaa tgttgctttt atgggttatc ctgtaattga
      361 agcagtttta ggtcatgaag caatattcta tactgcaata ttcaatttgc cttttaactt
      421 attttctttc actttaggaa tttatctttt atctaaaggc agtacaagta gaggtttttc
      481 aataaaatca cttataagcc ctgctacaat agctattgtt attggacttt ttttatttgt
      541 aacaggactt aggttacccc aatttataaa cgagccactt gagatgttgg gaagtataac
      601 tacaccaata tccatgatta tcattggaag cttacttgct aattcttcag ctctcgattg
      661 ttttgtcaac aaaaaattat atatagtcac ttttataaga ttattggtac ttcctgtgat
      721 tgtgtatttt attctaaaag gatggataaa tgataagatg attttagcta taccagtagt
      781 aatttcttca atgcctgctg ctgcaaatac tgctataatg gctaatcaat atgactccaa
      841 tataacacta gcttcacaat gtgtgttttt tacaacacta ttttcagtta tatctatacc
      901 ttttataagt attttactct tatcttgatt tattttttta tgtattatat aataacatat
      961 tcaataaatt acaaatataa acattttatt gtagtatatt gatggaattt tttcctagat
     1021 atttgtcaat aatatctata tgtattatag aataggaggc ttaaatatgg atatggattt
     1081 actctttaaa atagtatttg ctatagttgc tataggtgtt tctgtaaaaa tcattaaaac
     1141 tgttgctagt ttagctttta aaatagcgtt gatattgcta ataattttat ttgtatatag
     1201 aatgtttttg taaacattta aaaataagtt ttttaacaca atagagaagt aaaatagatt
     1261 atctttaatg taaatttttt attaaagaca ctctatttta cttctctatt agcttcttta
     1321 aattaaattt ttattatatc ttcccataat ataaacatct tcaaattttc catctctgat
     1381 tacagcatat tttttagttc cctctatttc aaaacctagc tttttatata aatttaatcc
     1441 cttttcatta tccacaatta cttctaatcc tactctagtt aacataatcc aattatctgc
     1501 tatatctaaa agttgttcca taagcttctt acctactcct cttccctgat aattctctgc
     1561 aacacttatt cctatttctg cgctatgtct caatcgtgga gaagaattta catgtaaccc
     1621 agcaaaacct attacatctt tttctccatt attattagtt tcagctacaa acatataatc
     1681 attttctccc aaagaagcta acatagcttc agttttatcg actctgtcac tcattaatgc
     1741 caaagtattt ctcataacac tgtatgttct tctaatttca ttaaaagctt gtgcatcttc
     1801 tcttcttata ggtcttattg ttatatctat aggcttactc atattggatt ccccctcaaa
     1861 cataatcttt atttttaatt aaaattttac atctttattt tatactgttc aaatctatgt
     1921 atgcttctac ctgaggtaca tatatcatat tatcttttat atgtgtactt ccaaattctt
     1981 ttaattctgt accttcttta tccttagtta ttttatatct aatggattcc atgccttcaa
     2041 ctacttcagc atgaaaaaac ataccttcat attgtttaaa tctaaaatta aaattgtctt
     2101 tagtaagttt tattatcata tttttatcta ctccttatat aattgatatt tttcttatta
     2161 ttagttgtat atatactgct tattcatatt aatatattat aacttatcat ttatttagta
     2221 taaagttata aatagatata tttatactta ttttacaatc tttcttcaat tgatatataa
     2281 tatgtttata taaactaatg tatgtgaggt gaaatatgag caaaaaaaca aaatattttt
     2341 ctttaattct ctttcctatt gctttgcttt taaattttat agccagcaaa gttccaaata
     2401 ttgttgaaaa atattactcc caattcatag ataaaataat tgtacaaata ttgagtaaag
     2461 tttctggaat tttcccattt tctctctatg agattacaat gtacataata gttatatcta
     2521 tcttcttatt tttatgttat actttatcta caatttttaa taagaaaaaa aaattaaaca
     2581 tatatttaaa aaattccatt ttaaatatat tatccattgt atcaatagtc tactttcttt
     2641 ttgtagtgct ttggggaata aactataata ggattccact agaaaccaca cttataaata
     2701 actataattt aaaaaacaat acctctatcc aatcaaaaca atatagtgtt aaagagctta
     2761 ctaaattata taaattcctt gtaattaaag caaatgaaac tagaaaactc acattacaag
     2821 ataaaaatgg tattgtaaaa tctaatacag attataatgg tgtcataaat agagctcaac
     2881 taggatacga taatatcttg gatatattac ctagtgttag tggtagttat tctaagccta
     2941 agtatattat ttcatcaaat ctaatgtgct atacaggaat aactggtata tactttcctt
     3001 ttacaggtga ggcaaatgta aatatagcaa tacctgactt atatattcct tgtacagttg
     3061 aacatgaaat ggcacatcaa aggggatttg ctagtgaaga tgaggctaat tttatagcgt
     3121 atcttacttc tataaaacac cccaatattg attttaacta ttctggatat attttagctt
     3181 taaattatac tgcttctgct ttaagtaagg ttgattataa tgcatatgtt gatatttctg
     3241 ctggcatttc agattctgtc cgtagagatt taaaaaatga aagtgaattt tggcaaaaat
     3301 atgaaggtaa aattaatgaa atttctaatg agtttaacaa ttcttactta aaagctaatg
     3361 gcgttactga aggtactcag agttatggta aaatggttga tttattgcta acttattatg
     3421 aattatatcc atataactaa acactacaat tcaaaatttt attcatatac agctgatatt
     3481 aaatcagctg ttttctattt aaataaatta tttaacttca agcaatctta tataatttga
     3541 tagtacttat actatctgta taacatatca attctaacta ttttagttaa aacatttcta
     3601 ttatcttttt taataaatgt tttaactaaa atttttaaaa cataaaactt ccacattatt
     3661 tcactatcta tataaaaatc taaaaattca catataaaat tacataacta aaaaatcata
     3721 aatttataga aagcatatat tcaaaataaa aaagcatgta tcaaaacttg acacatgctt
     3781 ataatcttat gtatatatat cattttgtca caataaaaat ttacttgtaa cactgatatt
     3841 tttaaacaaa atagtcctta tttaattaaa ttaacattaa ttaaattcgt actcattttg
     3901 ctatcattaa acattactta tagtaatttt ctaatacact atttgaaact tccttccctt
     3961 gaaaactttt caaccaattc tcatatgctg agtttttcca tacactacca tcattaaatg
     4021 ttacttgttt tacacaagat ataatgtact ttacaccatg ctctccagtt gattgtttcc
     4081 atttatcaaa caatagaaag gcatcatttt gattcttacc tggtgctatt gcattttgct
     4141 ttgcatattc tttctccatt tgtttccatt ctttcaacca attttctacc catgccttgc
     4201 cttgttgttt ttcaaacata cttattatat cctgtggtgg tgattgttgc atttttctat
     4261 aagtatgtga atatgatttt ggagacacag gagatatgcc tgtaacaata ccatagtcta
     4321 caccagctgg gctaaatcca acacttccta cttcgccatc tgctgccaca ttttgagcat
     4381 cccaatatag ctcaagtggt tttccatttt tatcatatgc taagcatgta atttcataat
     4441 ccataattgc cttttcactg ctatttgtta agcttataac attgctaggc caatgatatg
     4501 gagtatcatc atactgtatt ttgctattat ttataaataa tggaatattc tcattataaa
     4561 caagaccttt actactttga atatctgata ccttctttgt gacagtactt accgtatttg
     4621 ctaagtatac atttgttttt tctgaattgg tcgtactaag agaaaatgtt gttgtcgttc
     4681 cagtaatcag caaagcaaca actaaaatag gtaagctaga tattttttta tatctcacag
     4741 ttcttcacct ctttttctat taacttataa taaaacaatt taccaaaaca ttaaaatcta
     4801 tgttaacata atttaccaac tgttctagta actatatcct atcatagtta ctatcaagtt
     4861 tcaatactat tgatggaaat tttcactata ttaaatcttt ttttaatttt ataa
//
LOCUS       DAEMHZ010000203         4831 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000203 DAEMHZ010000000
VERSION     DAEMHZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.204"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..639
                     /locus_tag="KPK72_003623"
     CDS             28..639
                     /locus_tag="KPK72_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG2490791.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            937..1275
                     /locus_tag="KPK72_003624"
     CDS             937..1275
                     /locus_tag="KPK72_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG2490792.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1682..3118)
                     /gene="miaB"
                     /locus_tag="KPK72_003625"
     CDS             complement(1682..3118)
                     /gene="miaB"
                     /locus_tag="KPK72_003625"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG2490793.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3366..4802)
                     /locus_tag="KPK72_003626"
     CDS             complement(3366..4802)
                     /locus_tag="KPK72_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG2490794.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSNTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1625 a          744 c          529 g         1933 t
ORIGIN      
        1 ttaataatat tattttagga ggtaaaaatg ataaacgata ttaaaaataa attcaaaaat
       61 tacaaaccat atataaatgg atacaagaat atgaagagag catctgtttt aattccaata
      121 gtagaaataa ataacacaca ctatatatta tttgaagttc gctcaaaaaa tttgaagcat
      181 caacctagcg aaatttcttt tccaggtggt aaaatagaat caggtgagac tccatatgaa
      241 gctgttatta gagaaacttg tgaggaactt ggaactttta gtgacaatat agaagtaata
      301 tcaagtcttg acctccttat aacaccagta aagtttatca tacatccata cttaggatat
      361 ataaaaaata ttaataattt aaatataaac aaggatgaag ttgaccatac ttttttggtt
      421 cctataaaat atcttttaga gaatcctcca aatacttata gtaatactgt aaaagttttt
      481 ccagatgaaa atctaccata tggttcaatt ccaaaccaaa aagattataa atttgaaagt
      541 ggaagttatc ctattctgtt ttataaatat aaagattatg taatttgggg aatgacagca
      601 aaaatactag aaaacttttt aaatttttta tcagaataac actcataata aattatcaaa
      661 acttttacat aagaaattta taaaagtttc taaatttaac aattctaaaa tatcaataac
      721 tatattttgt agcacttttt tattttatta ttgtttgaat atttgatggt tttcaatgca
      781 atttcaacat aaatactatg tattagtgta tactgttgat gaattaaagt atgataatct
      841 atttaataaa gtatgaagta aatgatttta atgtgccttt atggtaaaat tactaataag
      901 taataaaatt aattaaaata tatggaggtg cttataatgt ttgctgtagg atttgttgtt
      961 ataattgcac ttgccttttt cattttaagt gtactattat ttaatggaaa agccagtatg
     1021 ctactagcag gttataactc tatgagtaag aaagaaaaag cacgatacga taaaaaaaaa
     1081 ttatgtagaa gttctagcat tgttactttt attgtctcta taatgctatt tattatggcg
     1141 tttttgggat atagaataga gagtggtcaa atggacgaaa aaaaaatggt tcccttttct
     1201 ataatattta taatagtcat actttccagt gtatttttta atatcctcta ctctaacaaa
     1261 aaatgtaaaa aataaatagt tgtaagttga gcagttaatt ataaaagatt gactgctttt
     1321 tcatgtatat tgactactct tattaaatat tttaacattt aaaaatctat gtaaaaataa
     1381 tgaatatagt acaaacttct aataatgatt ttacctttgt ggttacattc cactatatat
     1441 aatttaatat gataacaggg aattactaat gacaactaag taaactttta taaaatataa
     1501 taaaacaaaa atagatgtac aatgaaatta tatcttatat caaatttatt tttattaata
     1561 gcaaaaactt acattagcat aacatatgac aaattcgctt aaaataaaaa tttttaatat
     1621 aatttgttct atagtacatc tattttgatt agtttacata attttattat tttgtacaat
     1681 attaattatt tactaatatc ccatttaatg aaaaagttct aggttcagta attttgacca
     1741 taactaattt accaattaaa tcatcatttc caccttcgaa atttacgagt ttattttgtc
     1801 tagttctacc agcaaactta ttttcattat ttttacttct tccttcaact agaacttcaa
     1861 ctattctatc tttatatcca tcatttattt ctgcagaaat cctatttaca gcttctaaca
     1921 ccctattaaa tctatcatgc tttatatctt ctggtatttg attttccatt ttagcagcag
     1981 gagttccctg tcttttagaa taaataaatg taaaagctga atcatatcta actttttcaa
     2041 ccacatctaa agtgtccaat aaatcttctt ctgtttctcc tgggaaacca atcattaagt
     2101 ctgtagaaaa agcaatacct ggaacttcct ttttagcttt ttctattatt tctaaataat
     2161 attcctttgt ataatgtcta ttcatttttt tcaaaaggct actgcttcca cattgtattg
     2221 gtagatgtaa aaactcacac accttatcac aatctctaat tgcgtatatt acttcatcag
     2281 atatatcttt tggatgagat gtcataaatc gaaccctttc aattccttct atatcattca
     2341 cttttcttaa aagttctgag aatgaaactg gattttcaag tgtcttacca taagaatcta
     2401 cattttgtcc aagtaaagtg atttctttcg ttccatttgc aacaagctct tttatctcgt
     2461 taattatatc ttcaggagtt ctacttcttt ctcttcctct tgtgtatggg actatacaat
     2521 atgtgcagaa attattgcat ccatacatta tatttacaaa tgcttttaat tcaaattttc
     2581 tattacttct aagaccttct attacttctc catccacatc ccatacatcc actaacattt
     2641 tgtcagaatt tattgattct gtaagtaatt gcggaaattt atatagatta tgtgttccaa
     2701 atataaggtc tacatgttta tatttttttc ttaattcctc tacaacatgt ggttgttgca
     2761 tcatacatcc acatacagct attttcatat ctggattttt tccttttaga tgttttaatt
     2821 gtcctaagtt accatacact tttagctctg cattttctct aactgcacag gtgttatata
     2881 ttattaaatc actttcttca accatcattg acatttggta tcccatctct tctaacattg
     2941 aacataattt ttctgaatca tgttcattca tttgacaccc gaaggtttga gtaaacacga
     3001 gttttctctt tccagttctg ttgaagtatt cttcattttg tctgtgtatt tgttctatat
     3061 atttatcttg ttcttgtatt ttctctatag gtattgttac ttgttttctt ttgctcactt
     3121 ctattcctcc tagttaattt cttatattac acataacctt aattatatca aataagaaaa
     3181 atcttttaaa gatattatta tttttctagt tgtgttttaa tagtaaatct aatgatttat
     3241 atatactata tttctaaaat ataaaatcat ataattatta taataataaa aagatgtttt
     3301 aaattaaaac ttaaaacatc tttccttaaa taaaatatat gatttactat taaatacaat
     3361 tacatctatt cattttcaat taaataacaa tcatttaata ttaaatattg accttcctca
     3421 accgttatat atcttcttcc tgaaaaagta tcattagtaa ctattccctc tgcattacct
     3481 aagctatcat ttctaacttc ataatatgca tcagaagaat ttgacattat aatatattct
     3541 ccagcaggaa tttctaatcc aattttatac ataccatttc caaacttacc attcttggcc
     3601 ttctgggctt tagcatactt tgctaatgtc attgttgaat cctcaatttt aatatattga
     3661 ccattcttta aggtgatata tctagtccca ctaaaaatat catttgaaag tatactatca
     3721 gcattacctg tactatcact tgtaacttca taataagaag cataacttcc tgagtttgaa
     3781 gttattaagt attcacctgc tgatatatct tttccaacct tatacattcc atcttttata
     3841 gtctttgtag taggtttaac attttttaca accccagtat taatatctcc aattccattt
     3901 gttatattta aacactgttg tactatattt gaatctatat atcctataga agttatcttc
     3961 ttagcatttt ttaaaatgct tttattgcta ccatcattaa ctaaaaccat tggaccatgt
     4021 tttgataaag aagatcctac aagtgcattt gttaattcat aagctccagc tatataaaac
     4081 tctctagcat ccttataaaa cttatttatt attgctttat tagtttcaaa acgagtactc
     4141 ccccctagtc tagtagattt agtattgttt actaaagtgt tactcattga agctgttcct
     4201 cctattacat aagactttaa accactagtt gaaaaaggta tactttgtcc attagtaaga
     4261 attattggtg ctttatctct tgctgctaca gatgcaatac ttatagcatc tgcttctcct
     4321 ttatatgcat tggttagcat aacagtattg acttttttta ttgaatttat ttcttttgca
     4381 acattataac ttgtttttat tctgtcattt cccttaattc ttacaacttt cattttctta
     4441 gattttaatg aattttcgac agatgtacta atagaatatg ttcctccaat tatataaact
     4501 tttgatacat tttttaatct tgcagatgtc tcagttggta tagtattttt ttcagttaat
     4561 agtatagggg catttaaagc tcctgctagt ccacttgcac ttaatccatc tgcaatagag
     4621 ttactagtat taattaatat agcagtttta tatgcatttt tatctgctat tttacctgct
     4681 atttcatact tattttttcc ttgtattttt tctacagatg atattgcatc tactaattga
     4741 atatttgcaa atacaaaaga aatacttaaa gtcaatgtta atattctttt atagaatttc
     4801 ataataaaat tgtccccctc ataataaatt t
//
LOCUS       DAEMHZ010000204         4820 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000204 DAEMHZ010000000
VERSION     DAEMHZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.205"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1246..1953)
                     /locus_tag="KPK72_003627"
     CDS             complement(1246..1953)
                     /locus_tag="KPK72_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2490795.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(2828..3697)
                     /locus_tag="KPK72_003628"
     CDS             complement(2828..3697)
                     /locus_tag="KPK72_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2490796.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            complement(4001..4471)
                     /locus_tag="KPK72_003629"
     CDS             complement(4001..4471)
                     /locus_tag="KPK72_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2490797.1"
                     /translation="MKYQVSLLAVKDIEISKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT         1643 a          756 c          368 g         2053 t
ORIGIN      
        1 ttatacctta ccatatattt aaagcaaaga atttattagt ttttatttca cttaattaca
       61 tttaaacatt aaatcatcgt tataaagcta atctatttat catagtatat ccaatacctt
      121 taactttaag aaatatacac tttcaaatta aatagttgct tataagatta tatttaaaaa
      181 ctatttatat ttttactata tttaaacata acaatttagc tataacaaac aatcattcat
      241 tagttttaaa ttgacctact ttttaaaatc aattggttta tcttatttga ctttcaactt
      301 tatattatca ttgtagaata attctacata cttacgctta taaatagatt ttgaattacc
      361 tatcaattca aaaaaatccc ttataagatt cataagaata ttttattaat tgtaatacta
      421 tattattaaa tattcgcata ttaaatcaaa ttaatttaaa actttttaat tgatttatta
      481 cttttttaaa aactcatttt acataaattc atagcgattt ttataatttt atttattttg
      541 tagctgtata ttttatgttg tggaatttat ttctagtatt ttttaaaagg tctcttattt
      601 gtatcgagta taaagtcaaa aatttaattt gtcaatgatt ctttgcaata aaaatgtttt
      661 tttctacact tgatatctaa taatttactt atattatata ctattcatgt tttatgtcac
      721 atatatttat acttcatatg acatatttat attttatatt atgttaacat tatttaattt
      781 aaactttttt ctcaggtatc ttgtctctcc aaaaccttat taaaacaatt attaaaagat
      841 cttacgataa aaagattctt aataactatt ttacaaaata taaataaatc atccaaatca
      901 atattatata aataatataa ttatatcctc aaaatatcac tatcaatttt actatctacc
      961 ttaaaacatt atttttaaaa ataaatattt agtattaaag tatatatcac aaaattgcat
     1021 ttatagtttt aactttgaaa tatttttaac taaaaaaaat acccaactga cctaagcaac
     1081 tcgattgggt ataaataatc taaaaaatca tttttgtata aactctttat ttaacttaca
     1141 tttttattta ccttctactt taattcaact catatttcta tgcttcttat actctcattc
     1201 aacttatatt tctacttatc ttatactttt ataaattcat cacaattatt tagttctcgc
     1261 tatcaaagaa attgtattaa aatactttcc attctctact tccatcattt ttttgtcatt
     1321 tatagcatat gaattatcac aatttatcca atccttccaa gcttcatcat gacaatttaa
     1381 agcttcacat ttctctacaa tcacagactc cgattcagac caaagtttat tccaccatgt
     1441 aattgaatgg aagttcatat tatcttgcca aaatggaatg agttcatctg gtatacaatc
     1501 cacgaaatct tcttttaatc caggcattgc catagcaagt attcccccct cttttacaag
     1561 aggtgatata tgattgtcta aaaatccttc tttgtttcca aaatagtgat aagaatcgat
     1621 acttatcacc gcatcaaaga aaccttctgc ataaggtagt tcatgtgcct ctgcctgtat
     1681 tggaaatatt ttatcatcca atttaaactc tttaaatctt ttatagtttt cagttggttc
     1741 tatccataaa tctgttgcaa atacagtagc atcaaattcc tttgctaaaa atatcgaaga
     1801 tataccctta ccacaaccta agtctaaaat acgcattccc ttttctagtt ttatcttact
     1861 tgttaattct tccaatattt ttatacaatt aggtcccatc atatttttca tgagaaagtc
     1921 cttatcgtat ttattacttt ttgtaaatat cattctatct ttcctttccc tatcagttat
     1981 tatttattaa attttatacg tttgattttt tattttttat atgcttctta tattgagcaa
     2041 tctctttttt attcattctt taacagacaa ttttaatgtg taattttatc cactctgtga
     2101 tagtaacttt aatgaataat cttatctact gtgatagtta actttgatgg gtaattttat
     2161 ctactctaca ataatcacct taatgagtat cttatctact ctatgataat tattttagtg
     2221 agtaatttta tctactccac agtaatcgct ttaatgagta atcttatcca ctctatgata
     2281 gttattttag tgagtaattt tatctactcc acagtaatcg ctttaatgag taatcttatc
     2341 cactctataa tagtcacttt aatgagtaat cataattttt atatcatttt agatacttac
     2401 tctaataaga agtcattttc taatacttaa taagttttaa ttacttccac tttaacctat
     2461 atactaatta acttatttaa tttagtgcat atttgacact ttacctttta ttataccatt
     2521 tattatcata aatataataa ttctattttt ttaaaataaa tatttataat aatctaaaat
     2581 aaatatttat aaattctaat caatcctaaa taataatcta aaatacatca aaaaataaac
     2641 taaaaaatat cgaaaaataa tttaaaagac atcaaaccta aaccttctaa tcagcaatta
     2701 aaagtctatt ttcttagttt aaaaaataaa gatagatttt ttcaaaacac tggataaata
     2761 ggtaaaaaaa taaacagtta gtaaaataaa tttttactaa ctgtttattt ttattgttta
     2821 atatgtccta taaatcctct aagtaatatt ttccttgcaa aaattctgat tctaaaccta
     2881 gatttttaaa ataatcaacc atgcccatac acgcaaattt ttctgtagaa taatgagtag
     2941 cacctataac attaattaaa ttatcttttg ctatttgatg gaactctaat actggttcaa
     3001 aatgttttaa aggcttagta aacccagtaa tatatagatt tataccaagt tcagcaactt
     3061 ctcttgctac aaatggataa cttcctcccc ctgctgcaat agcaactaaa ccatctttta
     3121 atacagaatc tccataatca tacaatttca catcatgacc aacagatttt ttaattaatt
     3181 ccttaatctc ttctattgat tttagtgatg ttttacatat aactcctacc tcaattttat
     3241 cgtcatattt acaaaatggt ttgactattt ctaaacccag agcttttgca aaactaacag
     3301 aagttgaata gtcactatag ttgtctagtg gagaatgtaa tacataaaaa gaaattctac
     3361 gatttttcat ctcttccata taagatagtg gtatgtcgtg aaatggaaaa ccttcatctg
     3421 atgctatgta tcccattgca tgatgtgaaa aaattaaaat atctttttca tctttatcta
     3481 taacttgctt aattatttct ttatcaggaa aagtagttgt gtatacttta ttaatatttt
     3541 gagcattatc taatactaat cctatgtatt tttttctaaa ttctggagta ataaaatcat
     3601 tttcaaaatt cataaaactc caatcatcat taatattttt aatattaaat tctaaatcta
     3661 attttttata aagttcaatt gccttaataa taaccactcc tttataataa tattgaatta
     3721 ctacatatta atatagaaat ttccgataat aaactttata cgtttaagta ttcctacttc
     3781 aaattaaaaa tattataaat attcaaattt attaatatat tttaatatta tatttatttt
     3841 ttctttatta atattcccct tatttttatt tattttttct ttattaatat tttctttatt
     3901 ttaatttaat ttatgacatt tgtcaaatac tagtcctata tattatcttg aattagttct
     3961 actacaatta atttctacta aataattata atttaaacaa tcattcctta ctattttcac
     4021 actgctttac aaattcaatt ggatgttgaa ttattttaga agtttcttct acagaatatc
     4081 cctcagataa atatcttcta gctataactt ccatagactc tcctatctca ataatatgct
     4141 tatctgggtc ataaatacgc actactcttt gttgccattc atgttttagt ggcttatgga
     4201 caaactctac atctttataa taattgagtt tttcaataaa ttcgtcaaaa tcatcaacct
     4261 caaaataaag ttccatatta tttgattttt gtataattgt atctggatta atccctgtaa
     4321 gccatgcgaa gtcttcttgt atagcaaatc ctcctttaaa tgttacattt ttacctaagt
     4381 ccaattcaat ttcttgctca aataattctt tgtaaaattt ttttgatatt tcaatatcct
     4441 tcactgctaa taatgaaacc tgatatttca taaattttcc tcctaatttt ttatttctat
     4501 tttttcagta tcttctatta acattttttt ctctatctct tcagtacctt ttattaatcc
     4561 tttattttta cttttttaat atcttctatt aacacttttg tttctaccta ttcagtatct
     4621 tatatcaatc ttttattttt tcaatatctt ctattaacac ttttatctct atctattcag
     4681 tactttctat caatcttttg tttttatttt ttcaatatct tctattaata cttttgtttc
     4741 tacctcttca gtatcttata tcaatctttt attttttcaa tatcttccat taacactttt
     4801 atctctatct attcagtact
//
LOCUS       DAEMHZ010000205         4723 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000205 DAEMHZ010000000
VERSION     DAEMHZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.207"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            155..1306
                     /locus_tag="KPK72_003630"
     CDS             155..1306
                     /locus_tag="KPK72_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG2490798.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1352..2527
                     /gene="thiI"
                     /locus_tag="KPK72_003631"
     CDS             1352..2527
                     /gene="thiI"
                     /locus_tag="KPK72_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG2490799.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2683..3486
                     /locus_tag="KPK72_003632"
     CDS             2683..3486
                     /locus_tag="KPK72_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2490800.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIVSFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3729..4370
                     /locus_tag="KPK72_003633"
     CDS             3729..4370
                     /locus_tag="KPK72_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG2490801.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1896 a          450 c          917 g         1460 t
ORIGIN      
        1 aaaatttgta ataaagtgaa aaaactagct tttcatgaaa taatatttta taatttaaga
       61 ttgtcttttt aataaagatt aacaaaaaaa atactataat tagtggtata atagttagag
      121 ttgaagattt taaataaagg taggtgtaat taagttggaa atatacttag acaatagcgc
      181 aactacaaaa ccttatcaag aagttataga taaaatggtt tatgcactta atacggaata
      241 tggaaatcct tcttctgtcc atagaaaagg tgtagaagtt gaaaaagcta taaaagaagt
      301 aagacaagat atagctaaat ctctaggagc gaaagaaaag gagatttatt ttacatcagg
      361 tggtacagag tgtaataata ctataataag aggtattact agtttaaata aaaaaagaaa
      421 aaatcacata atatctacta acatagaaca tccttctgtt ttaaatacat taaaagattt
      481 agaagaagat ggttttgaag taacttattt agaggtcgga aaagatggga aaataaatat
      541 agaggattta aaaaatgcga taaagtcaac tacatgctta gtaagcatga tgcatgttaa
      601 caatgaaata ggaactatac agccaatagg tgaagttgga aagtatttga agggtttaaa
      661 agaaaaaatt tatttccatg ttgatgctat tcaatcatat ggaaaaatta actttagacc
      721 atctaaatac aatatagatt ttatgagtgt aagtgcacat aaatttcatg gtccaaaggg
      781 aattggattt atgtatataa aagaaaataa tcgccttaag cctatgttaa caggtggtgg
      841 tcaggaaatt ggaattaggt cgggaacaga aaatgtacct ggaatatatg gtcttggaga
      901 agctgttaga atactaaaca aagatttaga tgctgttatt tcgaaagtag ataacttaaa
      961 aaatatatta aaaaatgaaa taattgataa tatagaagat ataaaaatca attctccaga
     1021 agatggagta tgtcatatat taaatgtatc atttagaggg acaaaaggag aagttttact
     1081 tcattactta gagcaaaaag gtatttatgt atcaactggc tctgcatgtt cttctaaaaa
     1141 gaaaggtagt tacgtattaa atgctatagg tcttacaaat gaagaaataa atggaacaat
     1201 aagatttagt ttatctgata tgaatacaga agaagaaatg ttagaagctg tgaaagtttt
     1261 gaaagaatca atttgtgact taaggtcaat aatgaaaaga aaataaagaa tactgtataa
     1321 atagtgataa taaattagtt taagaggtga aatggtgtat aatatattaa tagtaaagta
     1381 tggagaaatt ggagtaaaag gaaagaatag atatatattt gagaataggc ttataagaaa
     1441 tataagaaat atgttaaaac caattggaag atttaatgta tataaagagt atggaagaat
     1501 atatgttgat ttagaagact atgattatga agaagtaatt gaagaagtta gaaaagtatt
     1561 tgggatagtg ggtgtgtgcc ctggagtaag agctaaaaaa gattatgata cattaaaaga
     1621 aatagcttta aaaatgttag aagaaaaaat agaggctgga tataaaactt ttaaggtaga
     1681 gtcaagaaga ggagataagt ctttcggatt gacttctcaa gaaatgagta tggatatagg
     1741 tggatactta ttatcaaagg taggagatag gattaatgtt gatgtaagaa atccagaagt
     1801 taaaataaag tgcgaataca gagaatttca tactatggtc tatagtgata ctatacctgg
     1861 atatggtgga ttgccacttg gaacgaatgg tagagctatg tctcttttat caggaggtat
     1921 agatagccca gtagctacat ggatggttgc aaaaagaggt atggaagtag aggcagttca
     1981 ctttcatagt tatccattta caagtgaaag gtcacaggaa aaagttaagg atttagctaa
     2041 aatactggca aaatactgtg gtagagtaag gcttcataaa gttaatatct tagaaataca
     2101 aaaagcaatt ggcgagaatt gtaatgaaga agaagctact attctttcaa gaagatttat
     2161 gatgaggata gcacagagac tttctgaaaa aagacattgt gatgctttaa ttacaggtga
     2221 aagtattggg caggttgcat ctcaaactat acaaggactt acttgtacta atgccgttgt
     2281 agacttacca gtgtttagac cacttatagc aatggataaa tcagatatag ttgatatagc
     2341 caaaaaaata gggacttttg aaacatctat agtgcctgaa gaagattgtt gtagtgtatt
     2401 ttctcctaga aagcccgtca ctaaacctag attagagaaa atagaaaaat ctgaaacagc
     2461 acttgacatt gaaaaattag tacaggatgc tattgacaaa atagaggtag aggatattga
     2521 gttttagcat acgagttatt taattattaa attgaatatt gagtatatta gtagttctgt
     2581 aagtatatta gtagttctgt tgaaaggatt tatgtagaca atttattaat atgtagtatt
     2641 taaaaataag tactataatt ttatttaatg ggaatgatta ttatgaataa aaagggtttt
     2701 acactaattg aattgttagt agttatatct ataataggca ttttagttat agtagctgtt
     2761 ccagcattgt ttagaaatat agaaaaaagt aaagcagtta catgtctttc taatagagaa
     2821 aatataaaga ctcaaattgt tattgcaatg gctgaggaat caagtaaagg caagaatgaa
     2881 gtcatgaaag aggtattaga aaacaaagat ggtaagtact ttgaaacaga accaaagtgt
     2941 aagtcaggtg gaatatattc agcaacgttt gatgatggtt atgatggaat aactggaata
     3001 gaaagcattg caaaagtgta tgttacttgt acaaaacatc cagacggtgt tgaaatggct
     3061 agggatgtgc atcaaagtat gaaagatttg attgtatcat tttcacaaga cccttctata
     3121 ataccaggag cttcaaaggg caatgatgat tttagaaaat atttattaga caataaatat
     3181 aaaaatgggt ggcctacaat tccagatgaa tttaaggcaa aatatggatt aagtaaggat
     3241 acactatata tacaaccata tgcatataat cctactaaat ctgatgctac tgtagttgta
     3301 tttgcaaata ataagactgg gggtaattgg tatacttccc tagtttacga ttatgatgaa
     3361 ggtagatggt ataaaggtaa aaatggtatt tctgttgcag gtaggtcatg ggatgttgac
     3421 acagatagtg ttaagtctgt aaaaacagag attcattcta aagagggatg gggtccttta
     3481 aattaatata tcatgtttta ttaatctggt attgattaaa ctatagaaaa gagcagtttc
     3541 tatagatgac gaataccaga ttttcattta tatatataat tgagatatat taaaagtgtt
     3601 tatattttta agtcttcaca tacaagctaa aaataattat aataatattg taaagaaatt
     3661 taattataag attaattttc tgaagattta gtatttgtac taagaaatac ataaaaaggg
     3721 gaatagatat gaataataat tttcaagaca accaaacaat aaatttaata caaagtcgta
     3781 gaagtatacg aaaatttacg acagaacaaa ttagtgatga gcaagtaaac acattattac
     3841 actgtgcatt tgcagcacct tcaggatgta ataaacagcc ttggcacata actgttgtgc
     3901 aagaccagaa acttttaaaa gagattagtg atgatacttt atctaggatt catgaggtga
     3961 gtaatgttga gataaataaa aactttaagt tattttatgg tgctccaacc gttttattta
     4021 tttcttatga tgaaagtagt tcatgggctc catatgatat aggaatactt acagggaata
     4081 taacaacagc tgcacaagca ctagggcttg gaagttgtat aataggaatg gttagaggtt
     4141 tatttacacc tgttgaacaa ggggatattg aaggtcttgt gagtgtattg gataaagaag
     4201 atgtaaaaga atcagagtca ataaagatga agtttgatac aaataaaaaa tacagagaac
     4261 tacttgatat accagaaggg tattctgtac catttggtat tgctgtaggt attccagatg
     4321 gaaatttacc aaatgctaga gaggtagttt acaaagtttc cagagtttag gataaagtat
     4381 tgcaagaacc aatcagttaa ttgaagaatt aaatcagtaa tatacaaata aattgatgga
     4441 agaggaagct atttaaaaag tagtttagat ttttcgttgt aaaataatta aataaagaat
     4501 aaggtgataa gtattttatt tattatctta ttctttttta tttttgtttt gtagaatgat
     4561 aaaaataagg attaagtgta ataaaataga aaacaagtat gttaatttta agtatatgct
     4621 ttaaaattta tccaagttaa aaatttaaga tatcttttca gtataatgga aaaaatgtca
     4681 aaatattgaa atagggaaaa aatagtgata taataagaaa aaa
//
LOCUS       DAEMHZ010000206         4722 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000206 DAEMHZ010000000
VERSION     DAEMHZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.208"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            653..1960
                     /locus_tag="KPK72_003634"
     CDS             653..1960
                     /locus_tag="KPK72_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490802.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2046..2308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2434..3237
                     /locus_tag="KPK72_003635"
     CDS             2434..3237
                     /locus_tag="KPK72_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2490803.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3275..3886
                     /locus_tag="KPK72_003636"
     CDS             3275..3886
                     /locus_tag="KPK72_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2490804.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3918..4589
                     /locus_tag="KPK72_003637"
     CDS             3918..4589
                     /locus_tag="KPK72_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2490805.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1900 a          427 c          836 g         1559 t
ORIGIN      
        1 ttagttaaat attaatatag aaattgaata ttatctagtt acaattattt ataactaaga
       61 taataaaaca acaatggtgc taatttatat gtataaatta attatgaaaa atttatataa
      121 gtaaaatata ttaacataaa ggtaaaattt tgtatactaa aagggaatat ttacagttta
      181 atggcagttt taaaataata aattaaagct gtcattttta tttaaaaatt tttagaaaaa
      241 gtacaatagt gttaatgtgt gaattagata aggtgtatat aatagtttag atgatatgat
      301 ataaaatatg ttgtaaatta aatgttttct ttgacaatat atacacaagg atgataatat
      361 ctatatgaat aaaagatagt gtgtaatttg ataatatgta attataacat tatgatataa
      421 ttacaataaa gtatgtaata atctgtagaa atttcaagct agttatctaa gttttcggat
      481 aactaaatct aaacggaata cataatgtat taaataaatg atgatactaa aatgatacta
      541 tttttataga aatttattga ggtatataat taatgaaaat aatatattaa attatataaa
      601 ttaaattata taatatattg agttttacat aaaagcaaag gtgggaataa aaatgaaaga
      661 aattgctgat gctataacat cagagtattt aagctatggt atcaatgaag atattagtat
      721 ctatgaaggt agattttgca tatatttaga taaaaaatat agatgtaatg ggaaaattta
      781 ttataaaatg acacctccaa tatctataag ttttaaagca gacataggtt gtgttgaaga
      841 gatagacaat gaagatgaca atttagcttt agattatgat aatgctattc ttgaagttca
      901 tggatataaa attatttcta taacaataaa tactttaagt gaatttagtg tagaaggtta
      961 tatcaatgat gactgcataa aatctaagaa ctcctatgtt gaatatgtag attttaatat
     1021 aataaactta gataaaatac ctggaaaact cattaaatac aatgataagg tatacgcagg
     1081 tagaattgaa tttgatataa atgattatgt tgtaactata gacaagcgat atgattatag
     1141 aaaagaatta aaatcagaat taaagtctaa atctggagca ataataactc atataggcag
     1201 gattagaaga aaagatggta gaatttttag aacaaataat acaataaatc ttttagatag
     1261 gatatcaaca gctttgagtt ttatgtgtgg aagatatgta gggttttgtt tggccaaagg
     1321 gtatagaagt ggtaatgaag tttatagaat atggaatgaa aatcaaatat ctccttttag
     1381 atatgtacca acatggtcag atacactttc aaattatcat aatatggaaa aatatataag
     1441 ccttatgtgt aaaaagctag aagattttta ttatggttct gctataaaga gtgttgttga
     1501 ttggtacata gaatctcttg gaagtgctac catggaaaac aatataatat ctgtacaaat
     1561 tgcacttgaa actttgtcat atgtaattct agtggaacaa aataagatac ttacagatga
     1621 agtatttgac tgtaatttgg catctaaaaa tataagactc ttacttgata catgtaagat
     1681 accatatggt aaacatgaat taaatatatt tgataatata ataaaaaata aatttgatga
     1741 tggagtggat ttagttattt atttaagaaa tagcatagtt catccaagta gaaagactca
     1801 tagagctgtg ttagaggttg aagatatttg gaatataatt tcaataggaa cgagatatat
     1861 tgaacttgtt ttactcttta ttttaggtta tagaggagaa tattcaaaca gattagtaga
     1921 aagatgttat ggtgaagtgg aagttgtacc ttggaactag atttttatat ttattttaaa
     1981 atatatattg acatatattg ccttatgcta taaaatcaat atcataataa taattcagaa
     2041 taacattaaa tgctaagaaa agaaatagta aaagtagtta atcttaaaca gagagtttcc
     2101 gattggtgag aggagcaata gattttactt tgaacacatc tttgagcagg actctgaaag
     2161 agtagaaata ctttattagg agatttccgg aatccttgca cccgttatag tgctagagta
     2221 taataatttt attgtactta aagaggttag tgtcgtgagg cattaatgaa cagaggtgga
     2281 accacgagag ttaactcccg tcctctagca gaagctagag gcgggagttt tttaatataa
     2341 ttttttaagc gcgcagagaa ttatattgca atagatacat tttttaaaaa ataatcaaaa
     2401 tattttaaaa attaaaagac gagggagaat attatgaaaa atatattaaa aaaagtagga
     2461 atatttacaa ttatgttagg tttattaggt ggagttgtag gttgttcaaa accagataat
     2521 gaaaaagata aggatgcttc aaaggaatct aaaaaagaag tagttgtagg gtttgataat
     2581 acgtttgtac caatgggatt tttagatgaa aagggagata cagtaggatt tgacgtagac
     2641 ttagctaaag aaacatttaa aagacttgga atggaagtta agtttcagcc tatagattgg
     2701 tcaatgaagg aaactgaatt aaatgattca aaaactgtag atgtattatg gaatggatat
     2761 tctataacag acgaaagaaa gaaaatagta tcatatacag aaccatattt acaaaataag
     2821 cagataatag ttactctaag tgactcaaaa ataaattcaa aagctgattt aaaagataaa
     2881 gaagttggaa cacaacaagg ttcaacagca cttgatgcag tggaaaagga taaagatttt
     2941 atgaatagct taaaaggtgg agcaccagtt ctttatgata cttatgataa agcacttaga
     3001 gatttagaga taggtagaac tagtgcagtt gttggtgatg aagttttaat tagatactat
     3061 atgggacaaa agggtgaaga caaatacaag gtattaaaag acgattttgg attagaagat
     3121 tatgttgtag caacttctaa agaaaatcca gaactttgtg agaaaataaa tgaaacatta
     3181 aaagaaatga aaaaagatgg aacttttgac aaaatttatg ataagtggtt taaatagaag
     3241 ataagttagc tgttaagaag ggggcactaa aggcatgtta caaggtttag agatagttat
     3301 agcaatgttt tgtataacat tgatagtatc aattccattg gggattggag ttgcattttt
     3361 aagattatca aagaataaat tagtaagtgg tattacacaa tgttatatat tgataatgag
     3421 aggtacacct ttacttttgc aaatgatagt catattttac ggattacctc ttttaggaat
     3481 tgtatttgac agatttacag ctggagtagt agctttcttc ttaaattatg cagcatattt
     3541 tgcagaaata tttagaggtg gaatacagtc aatcgataga ggacaatatg aagcttcaaa
     3601 agtactagga tttgataagt ttactatgta taagagagta atctttccac aagtttttaa
     3661 aagaatacta gcacctattt caaatgaagt aatcacatta gtaaaagata catctttagt
     3721 atatatatta ggattaaatg atattttaag aatatctcaa atagcaatga acagggaagc
     3781 aagtctgtta ccattatttg aggcaggagc gatatatctt atatttgtag ctatacttac
     3841 taagggattt gaattacttg aaaaaaaata ttcttattat agataaagtt acaatattat
     3901 aggaggaggt tttttatatg ttgaaaatca aaaacttaaa taaatcattt aaaaaaaata
     3961 gagtacttaa agatatttct tttgagttag aagaaggtca gattggagtt ttacttggta
     4021 agtctggtgc aggaaagacc acaatattaa ggtgtataaa tggtcttgaa gaatttgata
     4081 gtggagagat tataatagat aatgaggtaa taaaaaataa gagagatatg gcaaaaatac
     4141 gaggaaaaat aggtatggtt tttcaaaatt tcaacttatt tcctcatatg acagtccttg
     4201 aaaatataat tgaatcccca gttaatgttt ttaaagttcc tagaaaagag gctgaagaga
     4261 gggctagaga acttttaaga ttagttgatt tagaagataa attaaattct tatccatttg
     4321 aattgtcagg aggacagcaa cagagagttg ctatagctag gtcatgtgca ttgatgccaa
     4381 aagtcctttg ttttgatgaa cctacttctg cacttgatat tgatactatt caaagagtag
     4441 tcaatataat gaacagactt aaagacaaag gtatgacaat attaataatt acacatgatg
     4501 ttgtcttctc aaataatgtt gcagataaaa ttataagtat aaaagatggt atagtagaaa
     4561 atgttcaaat aaaagaaaaa attgtttaga gttttataaa tataaactag ggtagaattt
     4621 tataatataa ataaagggca agtaaaatca aatatactat gcccttttgt tatctcaaaa
     4681 tttaatctta tattttatgt ttaagttaca ttataattta ta
//
LOCUS       DAEMHZ010000207         4640 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000207 DAEMHZ010000000
VERSION     DAEMHZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.209"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            184..852
                     /locus_tag="KPK72_003638"
     CDS             184..852
                     /locus_tag="KPK72_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490806.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1011..1517
                     /locus_tag="KPK72_003639"
     CDS             1011..1517
                     /locus_tag="KPK72_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG2490807.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGNRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1645..2661
                     /locus_tag="KPK72_003640"
     CDS             1645..2661
                     /locus_tag="KPK72_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2490808.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRMAKADDTPFK
                     SEID"
     gene            3014..3718
                     /locus_tag="KPK72_003641"
     CDS             3014..3718
                     /locus_tag="KPK72_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG2490809.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEISVD"
     gene            4013..4492
                     /locus_tag="KPK72_003642"
     CDS             4013..4492
                     /locus_tag="KPK72_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2490810.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEHSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1952 a          386 c          773 g         1529 t
ORIGIN      
        1 tataagtatt tttatgctaa ctagaaacac ttaatttatg gcatttgtac ttgatttatt
       61 ttaatgtaaa taatttcaaa gttaatttta ttcaatatag tactataaaa gtataaataa
      121 catttaataa aaatatatat tatattaggt attttaaata aattttttaa agggtgattt
      181 tttatggaaa atataactag ttattttaca acaatactat gtgtatttat atgcttatca
      241 tctgtattta tatttactca actagctaga gtatttatta ataagaagaa gattaatcaa
      301 aaaattaaat caagaaacgg atttaggtat gatagagatt ttattgaagc cagaagagaa
      361 gaaatacata taaaggataa caataataat aaaaataaga gtaacaataa gaaattaaag
      421 gaagaaaaag tatttaaata cgataatggt gatttatata aaggtgagtt tgttgatgga
      481 aagaaaaatg gatttggaat atatattttt tcaagtaagg aaaagtatga aggattatgg
      541 aaagatgaca agatgcatgg aataggaaaa tatacttata gagatggttc aatatataca
      601 ggtgaattta aatacggatt aaaaaatggt ttgggaaaat taacatatcc caataatgac
      661 atttataagg ggtatttttt agataataag aaaaatggta agggtgtatt atataaaaat
      721 gatggaaata aacaagcagg gatatgggaa gatgatgaac aatgtaaaag tttagatttt
      781 aaagatttaa ataataataa aataaataat gtttatcaaa atagattaaa taacaataaa
      841 ttaaaagttt aattattcta ataaaaaagt ctttatttat gatggaatat aggatatatc
      901 tatgttgtaa tttgtaaaat cttcctatat catataaaat taagtattga ttagtctatt
      961 acataaatat agaatataaa tatatccatt taataataag gagaagagat atgaaagagt
     1021 atgtagttgt ggatttagaa actacaggat tagatccata taaaggttgt gaaataatag
     1081 aaataggaat aacagaaata aaaaatgaac aaattgtaaa aaactattca aggttgataa
     1141 aacctaaggg gataatttct tcatttataa cagaattgac acatataagt aatgaaatgg
     1201 tagaaaatga agaaccacta gaattagttt tacctagatt tagaaaatac ataggtaata
     1261 gaactataat tgcacacaat gcgaaatttg atttaaaatt tttaaattac tatcttagaa
     1321 tgttagattt agaaccaata aacaattata tatgtacagt agagttatta aaaaaatgta
     1381 aaagttataa aggaaaaaat aagaaacttg aaacagcttg tgcttattac aacatagaga
     1441 atataaatgc tcatagagct gacagtgata cattagcaac tgcaaaacta tttttgaaaa
     1501 taaaagatga atattaatta taaatataga gaaaattagt gaaaatatcc tttataaaat
     1561 tgacccttaa acttacaatt ttgttctttg atttattttg tagaacaatg tataatgtac
     1621 cttatatgaa gaagggagac taaaatgggt tattacaatg ataatgataa taataaaata
     1681 agacgtaaaa aaattagtaa cagcaaagat agtaataaaa aatctaaaca ttcttatact
     1741 ccggcaaata aaaaaaataa aagttctaag acaagttcta gtaaaagaat gagtgaaaaa
     1801 gtagattcac agaatcttat aaaaagagat agagatagtt ttacaagaga taatgtaaga
     1861 cctttaaata cagattacaa tcaaagagaa agaagaagag tcgagtataa acaaagagag
     1921 aaaaaattaa aaagtaaaaa aagaaaaaaa caaaaaagga ttagaatagt tagagcatta
     1981 ttatctatta tgttagttgc actaattata ttttcagtgg caggtgcaat atttacagtt
     2041 tcagctataa aagggtcacc agaagttact aaaaagttaa ttaggtctaa atatataagt
     2101 agtgaggttg taagtataaa acaaatgcca aatgatttaa aacatgcaat agtttccata
     2161 gaagatgaga gattttataa gcataaagga gtagatgtta tttctctagt taggtcagta
     2221 ttaaacaatg tgtttacaga tacaacacaa ggtgcaagta ctatagatat gcaagtttca
     2281 aaaaacctgc ttactagcaa tgataaaact atgaaacgga aaataaggga tatgtataat
     2341 gctaagcaga tgaataagat tatgactaag gatgaaattc ttgaagcata tttaaataat
     2401 atgtatctag gtaaaaatgc atatggagct gcaaagggag ctgaagtcta cttcggtaaa
     2461 aaagttagtg aattaaattt agctgagtgt gctatgttag ctggtatgac aaataatcct
     2521 gcaaggtata ttgaccatgg agaggctaag aagagacaag tgattgtact gtataagatg
     2581 catcagcttg gttatataac tgataaggag tatagaatgg caaaagctga tgacactcca
     2641 tttaaatcag aaattgatta aaattataag aatatagtat ttcaatagtt taaacaaacg
     2701 aaaatgtaat aaaaaaggtg atttgccacc catttgccac cataattata atttaatagc
     2761 aaattgtttt aatgtacaaa tccgataata taatattgtt ttaaatacta cattatgcta
     2821 aaatttaatt gtattttgta aattaaaaaa cttataactt agttttctag ggttccgcat
     2881 tttataatgg tcaggtccaa gaggaaacgc acaattgttg tgtacacgga aggataaaag
     2941 cctgggagat atttaataat atttctatag gcttttttat ttttacatat tttaaatttg
     3001 aggaggtttt ctaatgaaaa aatttgttat tgaggatgca ttttggaatc tatttcctag
     3061 tgcaaaaatt ggagttgtta tttgtaatga tatagataac tctataaaag atgaagacta
     3121 taagaaaata attttgaagg gagaagaaga agcattaaaa tacttggagg actcagaatt
     3181 tagcaacaac caagtgatta aagtatggag agaagcattt aaaaaattta aaacgaaaaa
     3241 aggagcaagg tcttctattg aagcattatt aaaaagagtt cataatggaa ataatatagg
     3301 aactattaac cctttagtgg atatctacaa ttttatctca ttaaaatatg cattaccttg
     3361 tggaggggag gatatagata aatttatagg agatattagg ctaactagag caattggtaa
     3421 tgaggagttt atcccactag ggacagatga aaattcattt ccatatgaag gagaaataat
     3481 ttataaagat gatggtggag caatttgtag atgttggaat tggcgtgaag cagtgagaac
     3541 aatgcttact gaagatacta acaatgcctt tttatgcatt gaattagtag atgaaagtag
     3601 atttaaagaa tttgaaaatg ctttaaatga attagctaaa atagttcaag aaaaattggg
     3661 tgggaattgt aaaatttcaa ttttagatat tgataataaa gagatatcag tggattaata
     3721 tggaggtgct gttgtaatgg actattctag ttataaagat acttatattt agggtaagaa
     3781 ggtctatttt agatataacg attatcaaag gatataatat agaaaataaa aatgtagaat
     3841 ataaaaagct tttaaaataa gaaaaaaatg tattttaaag gcttttatta tactcttttt
     3901 tatttgcata attaacaata aaaatatatt gttaattata atatttttat tgaaaaacga
     3961 tataatataa tgtattatac tatatataaa atattttaaa ggagttggca tgatgtctaa
     4021 taaacttata aaaaatttac taagtggtat attacaaatt ttattttttc ttttaggatt
     4081 agtaattgtt gtgggaggat ttaaaagttt tatgtacctc tgttttagtg gagaagcaac
     4141 tctacaaggc actatttatg gaatattaat gtttatttta ggtgtatcat attttatcat
     4201 tataaaatca ttgatagaag tattaggtag ttctgaacat agcctatttg taaaagagaa
     4261 tgtaaaaaga tttaggatta ttggatatct tttgctttta aactctctaa ttgagtttat
     4321 ttcaactttt ggtactactg gaaaaggaat gagatttcta gatttaggat ttggatttta
     4381 ttttactgtt ccagtatttg tatattttat aacttctctt atgagttttg taattgcaga
     4441 tggatttgta aaggcaataa agataaaaga ggataatgat ttaactattt aggtgtgaca
     4501 ctttaatata taggagataa ataaccagaa aagagtgatt aactagtaat ttaaaataaa
     4561 atatattagg gtataataca taaactataa tgctaaaatt aaaagattat aatactaaaa
     4621 tataattatg ttttaattaa
//
LOCUS       DAEMHZ010000208         4615 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000208 DAEMHZ010000000
VERSION     DAEMHZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.210"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            207..1979
                     /locus_tag="KPK72_003643"
     CDS             207..1979
                     /locus_tag="KPK72_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490811.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     KHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            2001..3422
                     /gene="ccpM"
                     /locus_tag="KPK72_003644"
     CDS             2001..3422
                     /gene="ccpM"
                     /locus_tag="KPK72_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG2490812.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKYNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3451..4521
                     /locus_tag="KPK72_003645"
     CDS             3451..4521
                     /locus_tag="KPK72_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG2490813.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
BASE COUNT         1897 a          406 c          724 g         1588 t
ORIGIN      
        1 taataaaaac taattatagt taaaaatata cctttcatca ataacagatt gaatatatta
       61 agattttatt ttacaattct attaacaatt ttattttaaa gatttaattt aaaatttaaa
      121 tatgtattat acataataaa ataatgttat attatattaa ttaataaaat attttatttc
      181 aattttatta agtatggagg tgtattgtgg aaaatggtaa atttcaattt tttaaaggtg
      241 taagaaattg ttttaatatg atttatctac atgataaaag atatttattt atctctatgt
      301 ttatggcaat ttttcagggg attgttccat ctatttcttt gttagtaagt caaaatatca
      361 ttaataatat gcaaacattt tcgaaaagtg taaaagaaat attagttttg ttaattatgt
      421 atttaatcat aaatataatt ccttcaattt ttcaaagttt ttatggatat tatagaataa
      481 agtttaataa aaattttgaa aaacatattg aaacaatgat gttagagaaa tctttaactt
      541 tgagtttaaa agattttgag gatgataata cttatgacat gataaataga gctcaatctc
      601 aaaaaggatt taatattact atactatctg aaaaccttat ttcaataata agagaatcag
      661 tatctacact ttctcttatt gttgtattat cacaatttca actttggcta ataatagttg
      721 ttattgttat tcctgtattg aaatcattat attccattaa attaggaaag gaacaatatg
      781 atgttagtgt aaacagaact tggaaagaaa gaaaggcctg gtacatagat tatttgttgt
      841 caaaaggtta tgcttacaaa gagattatgg tatttagaat tggttctaaa cttattaatc
      901 attataaaaa attaaaagag gatattatat ctcaagatat gaatatagct aaaaagaatt
      961 ttattgctac gctattatgt acagttttag attcaattgt atctgcagga attataggat
     1021 ttataataat tcaaggtatt ttaaagaaga ttttaatagg agatgcgact acatatatac
     1081 aagcagttca atctataaag gataatgttg aatcaatatt ttcgttattt agttcagtaa
     1141 tacaagatct tttttataca gatttactgt ttaaatttct agattataac tgtaaagaag
     1201 actcaattaa acatataaaa atatttgaaa aaatagggga tataaataaa atagagttaa
     1261 taaatatttc atataaatac aatcattcat cagagtattc tttaaaaaat attaatttaa
     1321 ctttaaataa aaatgatcct attgctatac taggaaaaaa tggttcaggt aaaacgacac
     1381 ttttaaaaat attattaggt ttttatgatg attatgaagg tgacatcttg ataaatggaa
     1441 aaaatttaaa ggatattgaa aaagatgaat acattagaaa agtaagttgt atgtttcaag
     1501 attatattaa atatgaggct acattaaaag aaaatgtcgc catgggagat gtatataaga
     1561 gtaacttgga agaaaaagtt aaaagtaaac ttaaagaaac taaattgaaa ccaagtattt
     1621 atgaggaaga tggattagaa actattttag gaacctggtt tggtaagaag caactttcta
     1681 tgggtgaatg gcaaaaagta gcaatatcta gaactcttat gaaagatgct gatatgtaca
     1741 tttttgatga accagattca tctttagatt ctgaagcggg aaatgaactc ctttctttat
     1801 ataaaaaaac atttgaaaat aagattggaa tatttataac gcaccatatc caacatgcaa
     1861 aaaagataac taacaatatt gtagttcttg aaaatggaaa gttgttagaa agtggaagac
     1921 atgatgaact tatggacaaa aagggtgttt attttaattt attttgtaac tgcaaataaa
     1981 tattaaaggg aggttaatta atgaatcaaa aaccatttat acacacattt aaagctggaa
     2041 aatcatattt tatttatgat gttaatacgg ataagatttt aaaggtaaat gctgctgttt
     2101 ataattatct aaataaaatt gagcataatt tagaaaaaga aagtgactgg aacatagaaa
     2161 tagaagacga aattaatacc ttgattaatt atggatttct aaaaaataaa agggtaagta
     2221 aaacaatgca ccctgaaact aagtatataa aatatcatat agaaaataat ctatcctcta
     2281 ttattttaca agtaacccaa aattgtaatt tacgttgtga ctattgcatt tattctggtt
     2341 catacagcaa cagagttcat acaaataaaa gaatgaatat agatgtagca aaaagaagta
     2401 ttgactattt gattaagaat tctagtcaat taaatgaagt tagtgttggc ttttatggag
     2461 gagaaccctt acttgagttt gatttgatta agcagatagt agaatatgta aaagtagttg
     2521 gagaaggaaa ggaaatatac tttaatctaa ctacaaatgc aactttattt aatgaggaaa
     2581 taatagaatt ctttgtaaaa aataatataa caactatgat tagtttagat ggaggaaaag
     2641 aaattcatga taaaagtaga aaatttgcaa atacaggaga aggatctcat gatgttgtat
     2701 taaaaaatgt acgttacata aaaaataaat atccagaata ttataaaaag aatattacat
     2761 ttaatacagt gataaattca gaaaatgatt acatttctat agatgatttt atatctaaaa
     2821 atgagacttt aaaagattca aactttttag cttccactct taataataac tattctgata
     2881 aagaaatagt ttcatcagat gaatatatat taggtaagag ttatgataga ttcttgactt
     2941 tactcaacaa attaaaattt ataaaagatg agagtatgtc acctttatca agaatagaaa
     3001 gagccttatt agatgatttt agatatgaga aaaatattga aggtagatta gagttaccag
     3061 atgtgtggca tcatggtgga ccatgtatac caggagcttt tagaatattt gtaactacag
     3121 atggagaatt ttttccatgt gagagagtaa gtgaaagttc taaaatgggt atgattggaa
     3181 atttagatag tggagtagat atagaaaagg ctgaaaaact tttaaatata ggaaaattat
     3241 atgaagagca atgttctgat tgttgggcct atagttattg tgatatatgt atagctaaga
     3301 ttgatgatac taaatataat gaaaatgaaa taaaaccaca tgattgtgac agaattagac
     3361 attctacaga aaataatatg aaagattact gcgttttgaa atctctagga tatcaatatt
     3421 aaattttcta atgaaaggaa agatgggatt atggaaaaag tacttatata tccatttaat
     3481 tataattttt ttactatttt aaatcattgc aataaaattt cagatataga aattaattca
     3541 ctggttagtc cttctggctg ggggttatgc aataaggagt ataatgttaa taatattatt
     3601 actaaagtta caaatgattt tcaaagtgaa cttaaaaaga attctttggt atggtttgtt
     3661 gattcaaaag aaaatctaga cttagaaaaa tttataattc ctaaattaaa agtagctcta
     3721 aaagaaggta aaaagatatt atatacacgt gatgatctta ataaaatatc taggtatata
     3781 tctcattatg aattcaaaaa gattaatgaa tggaatttag aaaaatatga aataagagat
     3841 atagagggta tatcacagat aaacacacca ataatattta tttgtggtct ttatggaggg
     3901 ttagatactt ttgatgtaaa tataaggata ggagaagaac taagaaaaag ggggttttca
     3961 atattacaat ttggccctaa aaaagagagc tatttattta aaatgattcc tataccagag
     4021 tttatgtttg atgattcgat aagtagtaag acaaagatta ttaattttaa ccattttata
     4081 aaagataaag aggtagaggc aaagccagat ttaataatta tagaagttcc aggagaatta
     4141 ttccctttct ctagaaaaat ggttggtaat tttggaatta gagcttttga aatttctaat
     4201 gcagtaataa atgattgtgg tatattatgt attccatcaa gtatatatga tagagaatat
     4261 tgtgaagaac ttaaatattt tattaaagga agacatggta taaatataga ctattttagt
     4321 atagagaaaa aagttataga tacatatgag acagaggtga agcaaaaatt ttcatatatg
     4381 tcattagatg aaaacttttt tgttgaagat atagaagatt atgaaaaaac attttttata
     4441 catgataata aacaattact agaattagtg gacaatatag tgaatcaatt aaaagagtat
     4501 gctaaagtaa tatctattta gaattggagg ttaattatga attacactga aaaggaatta
     4561 ctactagaga atatattttt tgatgtgact ggaattaatt ttaataaaaa taaca
//
LOCUS       DAEMHZ010000209         4463 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000209 DAEMHZ010000000
VERSION     DAEMHZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.211"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..867
                     /locus_tag="KPK72_003646"
     CDS             154..867
                     /locus_tag="KPK72_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG2490814.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            985..1854
                     /locus_tag="KPK72_003647"
     CDS             985..1854
                     /locus_tag="KPK72_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2490815.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2009..3154)
                     /locus_tag="KPK72_003648"
     CDS             complement(2009..3154)
                     /locus_tag="KPK72_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2490816.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(3487..4263)
                     /locus_tag="KPK72_003649"
     CDS             complement(3487..4263)
                     /locus_tag="KPK72_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2490817.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         1634 a          568 c          699 g         1562 t
ORIGIN      
        1 gtattattaa gtggggttaa gcgttataat taattataaa tattgaataa tctaaatgga
       61 taagataatg gtattaaaaa ttatcttaaa aggtgaaata atctgtattt aatgtagtac
      121 agttatatct ttaaagcaag gtggtgaaat gatataaata agatatctaa aagagggttt
      181 gatgatagtg ataaaagatg gttttcaagt aaagaattaa caagacttat aaaggctaaa
      241 gaagaaatag aatggttatt aaatagggat tacaaaatag acccagtcgt tacttttgtt
      301 ggtgatagat atcagttttc tataagacaa agagatgcat taaaaagggc agtctgtact
      361 gatgaaaaaa atattataag acaatcaaaa aggctttctc tagataaaat caaagaagat
      421 accgtaaata tagatggctt taatttgata atatccatag aggtagcatt atctggtggt
      481 actctagtaa taggaagtga tggcaatata agagatttag caggtcttag gggaacctat
      541 aagattatag ataaaacaga agaagctttg aatctgatag gtaaattttt taataaatac
      601 aatgcacaga atcttaaatt ttatttagat gctccagttt ctaattcagg aaatttaaag
      661 tataggatat tagagcatgc caaaacttgg ggaattgaaa ctgaagtaga attagtaaaa
      721 aatgcagatg ttgtattgga aaaattagat agagtagtta gtagtgatgc agtcatagtt
      781 gacaagtgta taagttattt taatgtggct agaggtataa ttgaagagta tattaaagaa
      841 tgtaatatca taaatttaaa taaatagata aatataggtg agaatatata agtatatgta
      901 gcaatattat tagtaataca agtaaagttt gaaaatagga gaacgaatac atatcgaatt
      961 tcataaaatg ttgtaaaaag acatatgaga ggatggatat gtgtgaaatt aactaaacta
     1021 aatattaaaa aatatagagc acctatatat aaatatgctt tagcaaatat aaatttgagg
     1081 tctgctaaat ctaccaattc aagtatcata acagtaatac ctcagggagc caaaatggaa
     1141 gtattggatg aggaagatga ctggattaag gtgatgtaca attctcaaga aggatatgtg
     1201 tataaggatt tggtatcagt aagcgaatat gcttggagta acttaaactt aagagaagat
     1261 aaatctacaa cttccaatat aattacagta attcctgaaa aatctagggt tgaagtcctt
     1321 caagtagatg gagattggag taaggttgta tatgatgata aaataggata tgtttttaat
     1381 tattttttat caattgatgg gaataaacca aatgaattag attataagta tttttatact
     1441 gacatgatta aatttgttaa cgaaaataat attaaaagta ctagcgatta tctaattgta
     1501 acagacctta gaaataaata tacttatatt tttaagaaag ataatggagg atggggacaa
     1561 ctttacaagt ggcaatgtac tataggcaag cctgagactc caactataac aggtatattt
     1621 tatataagtg gaagaaaacc ttcttttgga acggatgagt attcagttaa atatgctact
     1681 agaattaaag gtggatatta ctatcattct gtgctatatg attcaacagg aagttatata
     1741 atagatggca gacttggtga agctcttagt catggatgta taagattaag tacggaaaat
     1801 gcaaagtgga tatatgataa tataccagat acaacaacag ttataattca ttaagaaaaa
     1861 ttttgagttt tttattggga taatgattct taaattaata ggattatata ttaacttctc
     1921 ataaagagta ctatttaaaa agtgtaataa gagagtgcta ttaacaaaac aaagatattt
     1981 tgaggcactc tcttataaat atacataatt aagatatttt cataaacaat tctataatct
     2041 tatctttatc aaagaaaaca ggattagcac cagcacaagc atctttcata gagttttctg
     2101 caagcaattc aaaattagga ttatcaacac ctacatcttt taaagatttt gggattccaa
     2161 cctcttcaga aagagctttt atcttatcta ttacaaagtc aacacactct ttatctgatt
     2221 tattatttac atccattcca ataacttcag ctattggtcg gaatttggca ggggctgatt
     2281 tagcattttc ctcctcaact attggaagta gcatggcatt acatactcca tgaggtaaat
     2341 catataaacc tcctaattga tgagccatag catgtacatt tccaagacct gcattactaa
     2401 aggcaattcc atttaaaaaa caagcataac acatttgttc tctagcttct atgtcattac
     2461 catttttaac agctcttgga aggtatttaa atatctgctt tatagcatat aaggcagttg
     2521 aatctgtaac atcataagca ccattagcta ctacagcttc tatagcatga gttagtgcat
     2581 ccataccagt tgctgctgtt aaattagatg gtttgctaat cattaattct gggtcattta
     2641 ctgtcattgt ggctagtgaa ttggtatcaa ccattatcat ctttacatgt ctgtcttcat
     2701 cagttataac atagtttatt gtaacttcag cagatgtacc agcagtagta gttatagcta
     2761 tgataggaag acatttttta gaagttttgt tgataccttc atagtctcta atatctcctc
     2821 cattagtggc taatatagag atagctttac cacagtcttg tggagaacca cctcctatag
     2881 ttataacaaa gtcgcaattt tctgatttta atattttaag tcctttattc acattagtta
     2941 cagttggatt ttgtttaact tcatcataaa caacaaagtc tacatctatc tgagataaaa
     3001 tatctgttac tttttttaca gtaccatttg aaactagaaa tttatcacta acaataaatg
     3061 cttttttagt tcctaatgat ttaattgaat ctttagcttc attaagacat cctttcccaa
     3121 gtaaatttat tggtggtaca taataaatgc tcataactaa aatctccttt tattttcatt
     3181 gatttttatg taaaaatata tatctaacaa ttttcactaa tttactcata atatatcgta
     3241 tcttagattg agatagggtt gctggttctt atataagaac ttaattcgta attacgatta
     3301 tgattagaat ttaacatagt atttttatca tgtcaataaa aatataaaaa atattataaa
     3361 aaaaacaaag ttactgtagt taaatcagca actttgtttt tatagtataa atcaaataaa
     3421 tacattagtt taaaacaaaa tttttaatta ttatgttttg atatcttcca tatacagaat
     3481 attaatctaa tttattacaa tgtcttcatt acgttcttcc atcattgatt gaatattttt
     3541 ctgagcattt aagagtaatt tttttatcag ttctattttt tcgtcattag ctctaaataa
     3601 aggcagtgtt acactaacag cagctcttat acatccattt tttcttattg gtacggcaag
     3661 acattttagg tgttccattg attcctcaca ctctgaagct atttgtgttc tttgaacttc
     3721 aagaagctgg tcgtataaaa catcaaaatc tgtaattgat ttttctgtaa aaccttttag
     3781 accatctgga tataagctta ttagtttttc cttgtcaaaa tcacataaaa gagcttttcc
     3841 aagacctgta caattagcag gtattttttt tccaacataa gaaataagtc taattggttc
     3901 tggagaatct acttttccaa tataaagaac ttcattattg tcaagtatac caagctgaca
     3961 agtttcagaa caggaattaa ctatattagt catttcgtca attatatagt taaaaatagt
     4021 tcttttttca aaaaatgaag accctaatgt gtaagaacgt atccctatag aatatttgga
     4081 tgtgttttta tcaatagtaa tgtattttct agcattaagt gtatgaacta taggaaagat
     4141 gctacttttt ggagcattga taacatctgc tatttctgta agagtatatc cattttcgga
     4201 ttctgctaat gcttctaaaa tatctaatat cctagctgtt gggcgatgat tatctgaagt
     4261 cataaatttc cccctctttt tattaattta aagtattaga atatgttgat tttagcacac
     4321 tatacgatat tttacaatgt agaaaataaa tatgtaaatt tatatataaa taagctcttg
     4381 atttagatac agatagatat ttttttagac tgtacattaa ggaataacta atttccaaaa
     4441 tatacctaaa gaaagtaagt gat
//
LOCUS       DAEMHZ010000210         4426 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000210 DAEMHZ010000000
VERSION     DAEMHZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.212"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..110)
                     /locus_tag="KPK72_003650"
     CDS             complement(<1..110)
                     /locus_tag="KPK72_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB family sortase"
                     /protein_id="HBG2490818.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTK"
     gene            242..576
                     /gene="rnpB"
                     /locus_tag="KPK72_003651"
     ncRNA           242..576
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPK72_003651"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            854..3436
                     /locus_tag="KPK72_003652"
     CDS             854..3436
                     /locus_tag="KPK72_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG2490819.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFTDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGS
                     LKVEKTAKETAKEYEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTY
                     IAMYDKYYDYKLEELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEI
                     VEGA"
     gene            3559..>4426
                     /locus_tag="KPK72_003653"
     CDS             3559..>4426
                     /locus_tag="KPK72_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490820.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKN"
BASE COUNT         1991 a          406 c          788 g         1241 t
ORIGIN      
        1 attttagtat catgattgta tttagtaagt tttgagtata tattaaatcc actgtaaagt
       61 attactaata ctagtataat gttaattact attctatata attttttcaa tatatcacct
      121 cgagttaaat gtagtttata tatctatatt acactatttt tgaataaatt taaattttta
      181 gtataatatt actttgacat gtaaaagaat ttgtgttaac ataactaagt aaaataaata
      241 ttaagtaagc cagataatcg catgtaatct tacatgagga aagtcggagc tccatagagc
      301 agggtgctag gtaacgccta gtgggagcga tcctaaggat agtgcaacag aaataaaccg
      361 ccactattgt ggtaaggatg gaaaggcgag gtaagagctc accagcagct aggcgactag
      421 ttggctatgt aaaccccacc tggagcaaga ccaagatagg aataaagagt gctgctcgta
      481 ctctccggta ggtaggtcgc ttgagcctat tggtgacaat aggcctagaa agatgattat
      541 ctaatacaga actccgctta tagacttgct taattatttc gatagtcaaa aacatcagtt
      601 taaactgatg tttttttatt tgtaaatttg tctaatctac ttttaaaatt ttgaggaata
      661 ttgtaatata agttagtaca gatattaaaa atggtataat ataagataga tataaaaaga
      721 aaaagtaaaa tgatgtaaat aatatgatat gatataagat gtagcaaatt tagtaaataa
      781 ttatttagat atcataatca aattagcaac tttgcacaat gtaaatatta caataaaata
      841 attgatttaa ggagtgaagg ttttgagtga aaataatata ataagaaaca ctgttttaaa
      901 tgtcacagca agttttttaa aacaagagtc taaaataaat gaaaagctag atggtgtttt
      961 ggaaaagaaa tttgaaaaag tagaatttaa tgaagcaaaa tatgcagagc ttttaaaatt
     1021 taatatattg ttttataaga cattagcaag aaatacagaa ccacttattg gcaaatggat
     1081 agtagataag tacatacctg aaatagatga attagaaaaa gatttagagt taactacagc
     1141 aaaatgtaga aagtatgtaa ataaggctat gaaagaggga ttagactctc taaaagccaa
     1201 tgatttaaat agttttttag cttatgataa aatggaacta tcagagagaa gaagacgttt
     1261 agaaaaggat tataaggttt taaacctata taaagacctt cttaatatat ctcttagaaa
     1321 aataagttta gaaaagaaag aatgtggaga tttatttctt aaaaatcaag cagatgcgaa
     1381 aagagaatta aaaagagaaa ttatattctg tgtaaacaaa atattagcta gtaataaaga
     1441 tgtggtacct aataatgtgg aagagaataa cgaacaagac aaagtagaaa taaaagcaac
     1501 tgaaattgag gatttgcaat taggtaaaga taattcagaa aatgacatgc tactaaatga
     1561 aattaaaaat tcaaaagtaa aacatgaatc aaaatcaaat gatatagaat cgaagtttac
     1621 agataagtta aatagcttaa ataatgaaag tatatctcaa acaatacaca atgaatataa
     1681 gaagttatgt ggattagaag aacttcattc tgttgaagga tatggttttg gcaaagaaat
     1741 tattaaagat tttgcttgtg caacggttgt attagaattt ttaaagagaa gaaatagaga
     1801 ccttatagaa ggtgcaatga gactgactat aattggagaa tttggtcctg aaaattttaa
     1861 ggaatttgtg gactatgtaa ttaaaaataa aacagaaatt agtgaagata catggaataa
     1921 agcacagaat ctaataaaag ataactatag tgaattagaa aatcatgaaa ttgcatcaaa
     1981 aagaactaga agaaataaag atatcgatat tgaagaatac atttacatga taaaaaatgc
     2041 agataaagat atttgcttta gaagtagtat ttcaatagaa gatgatgctg aagaagggac
     2101 taaggaagaa gtagatagta ataatcaaga tataggtgac gttgtagaag ataaagatac
     2161 tacagataaa gagtacgaca gcaataaaga agatataatt gaaccagaaa ataaaaagag
     2221 taaaaagaag gctaaattat ttggatttat taaaaaagac aatgaagaag ctgaacaaga
     2281 agaagagaat ttaaatgata tttctccaga tataatttta gataaaccag ttgaaaatca
     2341 tcaagtgaaa tcagaagaaa tagagcaaaa tgaattgaaa gaaataaaac aagaagaacc
     2401 aagtcaacat atagaagaag aacgtagtgt aaaaatagaa aaaccaataa ataataactt
     2461 agatgaaaaa gtatcttcaa ataatgaatc gaagcttgaa aaagaaagta aaaacttaga
     2521 agataaaaaa gcaaaagaaa taaaagaaga aaaattagaa aatgaaaaat cagtagtaat
     2581 acctattaaa aagaaagaaa atagtaataa aaaatctaaa aattcttcta aggacaaata
     2641 tagagaaaac aagaaagaaa tgaaaaatta tgtatcagat aaagaagatt ctttagatga
     2701 tgaagaggtt gtttctaaga agagtagatt aaaagaaact attatagcag tagttatagt
     2761 ggttattgtt ggtgtaggat attttataac agtaggcaac aataagaaaa atgataaaga
     2821 gaatattcca aagtcatcaa ctcaacaaca agctaacaat aaattaactg aggaagagaa
     2881 aaaagcacaa gctgaaaaag agaaaaaaga agcagaagaa aaggcaaaag ctgaaaaaga
     2941 gaaaaaagaa gcagaagaaa aggcaaaagc tgaaaaagag gctcaagaaa aagcacaagc
     3001 tgaaaagaaa gcaaaagaaa tggaagcata taaagatggt aaaggtgtat attacactgt
     3061 ttatgcaggt tcacttaaag ttgaaaaaac agcaaaagaa acagcaaaag aatatgaggc
     3121 taaaggtatt tcttcaacaa taattcaaga aaatggatac tataaaataa aaataggtga
     3181 ttattctcaa tatggagaag cacaagaaaa atgtaatgaa ctagctaaga agtctattga
     3241 tacatatata gctatgtatg ataagtacta tgattataag ttagaagaat taaaagaatc
     3301 agctccttct ttgagtgctg aagaacttaa acaaaaatat gaagatttaa gaagtgagtt
     3361 aaagaacaaa tctggatatc gtgaatatgt taaacattta gataaacttt atgaagaaat
     3421 agttgaaggt gcttagagca ttataaaaaa ggagaaacta tgtctcagaa taaaaagtat
     3481 taaaaaaaat attatttgtt ttgagacatt ttattcacct ttataaaata cttataattc
     3541 tgtttaggtg gtgaaggtat gttgaaatta ggagaaaaaa tcatatatga gctttcagat
     3601 gggtttgagc ttagtggcat aagtagatac tcaaaagaat ataaaaagct tcatataaca
     3661 aatctaggga atataataat cagcaaatca gatgaagtta atgaggatac aggtgtaaaa
     3721 tatatatata gtttttcaga caataaagaa aaattagagg cagttttaga agattctcaa
     3781 ataattgtct atggaaataa cttgcctttt tacataaaaa gtgaaaattc aagtaaaaat
     3841 gttacagcaa atttgactat gaaaataact ttggatgaat atgatttaat ctgcaaaagt
     3901 caaagagaat ttatattcta tctaaaggat aatatgacta tattaagttt agatgacaat
     3961 aagttttata tgggtggaat taataaagat aatgagaagt ttatatttat cagtgggaaa
     4021 aatagatttg aaataaattt tgatgatata gaaagataca ttttagaaga taaaagagtg
     4081 tcattgaagg gatactttca tatggaaaga gaggggatta tagtaaggtc tgtatctata
     4141 tttaataaca atatagatag tgtagtccca tctgatttaa atgagagagt taaagataac
     4201 caaaaaatag gaaatcttcc taaagattgt gaaatagtat tctgcaagat atcaggaaat
     4261 atagatggat ttgattataa aaacacaaac atgcttttag taaaatatca agaccaatta
     4321 atttttataa ataagaaatc taagaagact attgtaaagt cagcgaaaga taattgttct
     4381 aagttaaatt taggagaaga tataatatta tatgataata aaaatg
//
LOCUS       DAEMHZ010000211         4405 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000211 DAEMHZ010000000
VERSION     DAEMHZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.213"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            196..3030
                     /locus_tag="KPK72_003654"
     CDS             196..3030
                     /locus_tag="KPK72_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2490821.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMVSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            3073..4317
                     /locus_tag="KPK72_003655"
     CDS             3073..4317
                     /locus_tag="KPK72_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2490822.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKEIDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         1731 a          581 c          663 g         1430 t
ORIGIN      
        1 ctctaattta atctataaaa atccatactt aatataatgc aactcttatt ttaacaccat
       61 ctattacaca aaacaacata ttaaaaagta aaatacaaaa tatccactat aaaccttatt
      121 ttaatactga atatatatta taatatattt aatgtagctt aaatatagtt taaacataaa
      181 gaaaggaaac ggtatatgct aagaaaagat aaagttctat tatctctaca taaattatgt
      241 gaaaatatga ctttaaaaga ccttcaatca gataaaacag gattcactac aatagaaatt
      301 gctaaccatt catctcttga tagaagtaat accagtaaag aattaaataa cctatacaat
      361 gaaaaaaaag ttctgaaaat atctggtaag ccaattttat ttctacatgc tagtattata
      421 gaaaatttaa ttggccgtac tttgaacagt gatgaatttt ctataaataa ttgtaatgag
      481 ctacttaaat caagtaaaaa tgactcagaa gatatttttt caacattact tggacatgat
      541 tctagtctta aactacctat agaaaaagct aaatctgcta tactctatcc tccaaaaggt
      601 cttaatacgt tactcatagg acctactggt gttggaaaaa gtacatttgc cgaaacaatg
      661 tacaaatatg ctgtagaaag taaaatcttt tcttctgaaa gcaaattcgt agtatttaac
      721 tgtgctgaat atgcagaaaa tgctaactta ttattaagta atttatttgg ttatgtcaaa
      781 ggttctttta ctggtgctaa taaggataaa ttcggcatag ttgcacaggc tgatggaggc
      841 atattattct tagatgaaat acacagatta ccaccagaag gtcaagagat gctttttctt
      901 ttgatggaca aaggcatata cagacgatta ggagaatctg acattgtgca taaagctaat
      961 gtctttattg tgtgtgccac aacagaagat atacattctt ctctgcttgg gactttttta
     1021 agaagaatcc ctgttacaat caacctacct gctttacagg ataggtcttt aaaagaaaga
     1081 ttatctctta ttaaatactt ctttattata gaatcaaaat acaccaatgc aactataaaa
     1141 gttcacaaag atgtaattaa atcattactc ctttatgatt gttttggtaa tataggtcag
     1201 cttaaatctg acatacaact tatgagtgca agagcctttt taaattacaa aactggactt
     1261 aaggataata tagaaataga tttatccctt gttcctgatt acatctatat tggtctttta
     1321 aaattgaatg aaaatcgtag taatctaaat aaattatcag aacttgatta tatgcttttt
     1381 tatgaattta atggtaataa cttagaagtt gaatatacaa aagaactatc taaatacaat
     1441 atagatacat caaacaatgg atttaaatac tctacagaga ttccttatct ggaaaatgat
     1501 ataaggagta tgtttataaa ttacatggaa aagtactcta caaaattact ttcgatatct
     1561 tcagtaactc attctgatta tacaagtaat gagattttta aagtaatcaa tccaaagatt
     1621 tactatgctg ttgaacattc tttaaaagag gctgaaaaaa tatcgagaaa caaatacaca
     1681 aaacaaactt atattgctct ttcaatgcat ataagtgcac ttatagaaaa tataaatcaa
     1741 gacacatata atcctaagta caatttatat aatgataaaa ttttcttaga taaagattta
     1801 aatatagcta aaatagtttt aagtataatt gaaaatgact tacaaacatc tttacctaaa
     1861 gatgaagtaa aatttatatc catgtttcta aatagcatgt atcttgaacc aaacaatcaa
     1921 tctgaaagta taggagtcat aatacttgct catggaaatt caacagcaag cagtatgtgt
     1981 gatgtagcaa attcattgct tggtacaaac cattgtaatg caattgacat gcctcttgat
     2041 gtaaaagtaa gtaccatact tgataaaact atagagttgg taaaagaatg tgacgaaggt
     2101 aagggtgtac ttcttttaac agatatggtc tctcttatag cttttggaga acttatttca
     2161 gaaaaaacaa aaatcaaaac tcgctcaatt gaaatggtat ctactccaat agttttagaa
     2221 gcagtaagaa aaagtacact accagaaatg actttggatg aattagctaa ttccttaagc
     2281 tctttaaatc catatattgg acgagcagca atcgattcaa cagaaataaa ctatcctgaa
     2341 aagtcaagag agtatgtaat aataactact tgtataacag gatatggttc agctatgaaa
     2401 gtagcagact ttataaattc ttcacttaaa aatattagta aatacaatat taatttagtt
     2461 cctcataata tagaaagttt taaaatgtat aagaataaat ttagtaatac taaaattctg
     2521 gcagttgttg gaagtgtaga ttttaatgta gccaacaccc cttttataca catagaagaa
     2581 ctactaaatg gatatggcct agatatgcta aacaaaataa tagaaggtac attcaatgcc
     2641 aatagtatta caaatataaa agaaacaaat ctatcataca acagtttaat aataaacttt
     2701 atgacccaaa acttagagct tttggactct gttaaattat tcaactatgt caattcttcc
     2761 ttatttacaa taatagaatt aatagatgaa aacttaaaga gcaaatttaa gctaggatat
     2821 atactacatt gctcttttat gatagaaaga tgttttaaaa atacaacttt tccatataat
     2881 aacattactg aattaattag taaaaatcat gaactatata gtttaatacg tgaagccttt
     2941 aagccaacag aagcttattt taatattttg ataccagacg aagaaattgc ttatataata
     3001 gacatgatta atgagtatct aaataaataa aatttatagc acttattttg tgcatattaa
     3061 ttaatatatg aaatggagga cgagattttg aaaggaaaaa taaaacagtt aacaatactt
     3121 gcattaattt ttatatttat aactcctgta tttgcatttg cagatactcc accagtacca
     3181 aattcttcaa gagcagcact acttatagac caggaaacta aaagaatatt atttgaaaaa
     3241 aacattgatg aaaaaatgcc attagcgagt ttaagtaaaa tgatgacctt tttattggca
     3301 atagaagcag ttgataaaaa tcaagttaaa gagattgata tggtgaaaat agacaagtct
     3361 acagcttcag taggaggttc tacttgtaaa cttaaagatg gtgatgaaat atctctagga
     3421 gagcttatgc agggtctaat gctagtatct ggaaatgatg ctgctatagc catagctaaa
     3481 catattggta aaactgaaaa gaattttgta aatatgatga acaagaaagc agaagaaata
     3541 ggaatgattg atacgtatta ttttaatcca aatgggcttc ctatatatac tgacccagaa
     3601 cataaagaac ctcctattga aaatatgtca actgctcacg atatagttac acttggaaag
     3661 tatatgtacg accattatga aaatcaagtt acaagaataa ccactatgca agtatataat
     3721 gatactaaaa aggattttac acattataac acaaatcctc tgcttgtttc agtacctggt
     3781 gttgatggaa taaagacagg atatacagat aatgctggat attgccttgc attttctatg
     3841 atggtaccta aagatgctaa aaacgaaaga aatcatagat tgataggtgt tgtacttggt
     3901 gatggtaaca agaaaaatag aatttcttct tctgctactt tattgaaata tggtaaagat
     3961 aatttccatt ctaaaaagat agcacataag ggtgatatta tagagactcc ttgtgttgat
     4021 ggtattgatg attttaagat aacagttaag gttgataaag atttgtatgg tgttgtttca
     4081 gataacgaga atatcaatcc taaggtagtg tttaaaaata tgaattatcc tatccataag
     4141 ggagatattg ttggtgttgc aaagtattat aatgactctg gtaaatttgt tggaagtgtt
     4201 gatgtaaaaa gtgaaagtaa tataggatgt attcctttga aggataagat taagattaag
     4261 gttgctaaga taaataaaaa acttgagatt aaaaattctg tttgttttaa agcatagtaa
     4321 agcatattat taaaagggta tgttaaattt ataaagactc cactttgtgg agtctttttg
     4381 ttttttacaa aaaattaaaa tagtg
//
LOCUS       DAEMHZ010000212         4373 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000212 DAEMHZ010000000
VERSION     DAEMHZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.214"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(226..2613)
                     /gene="cadA"
                     /locus_tag="KPK72_003656"
     CDS             complement(226..2613)
                     /gene="cadA"
                     /locus_tag="KPK72_003656"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG2490823.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISDEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2636..3001)
                     /locus_tag="KPK72_003657"
     CDS             complement(2636..3001)
                     /locus_tag="KPK72_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490824.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3200..4048
                     /locus_tag="KPK72_003658"
     CDS             3200..4048
                     /locus_tag="KPK72_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490825.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
BASE COUNT         1480 a          777 c          485 g         1631 t
ORIGIN      
        1 aataaacctt ctatgtctaa attttaaaat tacaatataa aaactctaca ctaaataaat
       61 acattaaata aacataatgg ataatgagta taaatttata tatgcaaagc gctccaataa
      121 aaaggtacca ataaagttaa aagaaactta ttgatacctt tcattattac atacttcaat
      181 atataattat aagtttttta aatagtatgg atttacattt atcccttatc tcatagctct
      241 catagcattt actactgcta taagtgccac acctacatca gcaaatatag cttcccacat
      301 agtagctact cctccagcac ctagtatcat aactataatt tttactccta gagcaaatat
      361 tatgttttgc cacactatct tattagtctt gtttgctatt tcaattgctt tagatatctt
      421 gctaggctca tctgtcataa gcactacatc tgcagcttca atagcagcat ctgaacctaa
      481 gcctcccata gctataccaa catcagcacg agctaatact ggagcatcat ttattccatc
      541 acctacaaac gctattttct ctttttcagt tcttccttca tataaatctt ctaatctatc
      601 taccttttca ttaggaagta aatttgagta aactgtatcc aattctaatt cttgagcaat
      661 attttttgct actttctcat tatctccagt taacattaca acttctttta cccctatttc
      721 ttttaagctt cttatggcat ttttactatc ttcttttact tcatcagaga taactatata
      781 acctctatat actttatcaa cagcaatata gactactgtg cctacttctt tagctgatga
      841 ataagatata ttttcttttt tcataagttt ctcattacct gctaatataa aattgccatt
      901 atgctttact ctaattccat aagctgctat ttcctcataa ctgtctattg tatctaaatc
      961 aattttttta ttatagtaac ttaatataga ctttgctatt ggatgatttg aatttacctc
     1021 cgcaatagct gcatattcta ataattcttc atctgatata ccttcaggat ttaacttagt
     1081 tacattgaac acacctttag taagtgttcc tgttttatca aatactactg tatctacact
     1141 tcttaaagct tctaaatagt tacttccttt aataaggata ccatttttag atgcaaaccc
     1201 aattccacta aagaaactaa gtggtattga taaaactaat gcacaagggc aagaaacaac
     1261 taagaatatc aatcctctat ataaccaatc actaaatacc tcacctgaga ttacaagtgg
     1321 tggcacaaat gctattaata aagcagctat tactactatt ggagtatagt atcttgaaaa
     1381 cttagatata aagttttctg tctttgattt tttaatgcta gaattttcaa caaggtcaag
     1441 aatcttagaa actgttgatt ctccaaaact ctttgtaact tcaatactca ataaagcatt
     1501 tttatttata acacctgata agatttcatc tcctttttca acttctctca gcactgactc
     1561 tcctgtgagt gcagaagtat ctaacataga aactccatct actactacac catctaatgg
     1621 tactttttca ccaggtttta ctactattat atcacctaca tttacctctt ctggtgacac
     1681 caccttaact tcagaattaa cttttagatt tgcatagtct ggtcttattt gcataagtga
     1741 agttatagac tttctggatt taccaactgc aacaccttgt aaatactctc ctactttata
     1801 gaaaagcata acccctactg cttctgataa ctcccctatt gctaatgctc ctactgttgc
     1861 tagagccatc aaaaagttct catcaaatac tcttcctttt gatatatttc ttattgctct
     1921 taaaagcaca tcaccaccta ctattacata ggcaactata aatactatat tgctaaactg
     1981 actttcataa cctgtagctg tttgatatat accaaatata tatacaagtg ctccaattat
     2041 aagtggaatt aattctttct tatttgtatc atttacagct cctgattttt cagtagttct
     2101 cccttttact ttgtcagtag accctacttg tatatcaagc cctggctctg ttgaatttat
     2161 tatttctttt attttattta taacatcatc ctcttcaaag ttattactta tatttactgt
     2221 aagtttttta tttacaaagt ttaaattaga agattcaact tcttttaatt tagaaacttt
     2281 gttattaatc tcttctgcac aatgagcaca gtttaatcct cctaatataa gctctttttt
     2341 aattgatgtt ttactagctg cattttcttt agaacttatt tgtatatcaa gacctggctc
     2401 tgttgaatct attatatcta ttatcttttc tatagtagta tcctcattaa aactttcctt
     2461 tatatttact gtaagttttt tatttataaa atttaaattg gaagatttaa cttcttgtag
     2521 tttagaaact ttattattaa tctcctctgc acagtgagca caatttaatc ctcctagtat
     2581 aaattctttt ttaattgaat tatttgcttc catatatatt cactcctctc atttatcatt
     2641 tataagtctc tctaatatga tttaatccac aatcaaatat ctgacttata tgactatcat
     2701 ctaatgagta atacactgtt tttccttctc ttctaaactt tactaatcta gcctgcttta
     2761 atactctaag ttgatgagat attgcagaat gagtcatatt taataaactc gctatatcac
     2821 atacacacat ttcatttgca aataatgcat ataatatctt tactctagta gtatccccaa
     2881 atactttaaa taattctgct aaatcatata acatttcttc atctggcata gttgatttag
     2941 cctctgttac tatttcttca tgtactatat tacaattaca atcatttatc tcttctctca
     3001 tttttaatcc tccattattt taatatatga ataactgttc atatgttcat tatatgttat
     3061 catttctaaa tttgcaacaa taatttttat taatttaata taaatagaat gcacatatta
     3121 taaacttttt taatatatat attattagaa taagtttttg taaacttttt tatagttttc
     3181 taggaggtgc aagtttaata tgagtaacaa gaaaaaaaag gatttagata ctagttacat
     3241 gccttactta gacccagcga actatgcaga ttatattact aatgaagaaa ttccccaaga
     3301 tgaattgaga aatgaccctc aaatttctaa tggttataca aaaataccta ataaccagaa
     3361 cataaataac ctatatccta ataatgtaaa tcattatcct aaaagtagta attctccaaa
     3421 caacttgcct aacacaaata atacatccgg aaatatgaat tacaatacat ctgctacaaa
     3481 taatatgcct aataacatga ataataatac gggtatgcct aataatatgt ctaacaatat
     3541 gaatagtaat atgggtatgc ctaacaatat gcctaataac atgaatagta atatgggtat
     3601 gcctaacaat atgcctaata atatgaatag taacatgggc atgcctaaca atgtgcctaa
     3661 taacatgaat agtaatatgg gtatgcctaa caatatgtct aataatatga atagtaatat
     3721 gggcatgcct aacaatatgt ctggcaatat gaataacaat atgtatggta atacaggtat
     3781 gcctaataac atgaacagta atatggggta taatatgaac aatactatgg gtattcctaa
     3841 cttctcatgt aatcaaaata tgcctcctaa tgtacttatg atgataccag gagttatatg
     3901 tcataataca atgcaaggta tgcctgtagt tatgcctagt actatgcctc ctaatatata
     3961 tcctactcct tatggttcta gtaatatgtc tgtacaaggt ataccacaag ctactaatat
     4021 tgaggaattt gatgaggaag aaatgtaata tatatttttt taagtaagtt gtataaaaca
     4081 ttaataattg agcataataa taaatgtatt cctcataata ttcccccaat attattatag
     4141 gtatagaaag ttgactgaat atgcgcttat tcggtcaaca gatattaaca taatctaata
     4201 agataaacat agaaaaagtc agataaaatt taattttatc tgactttttc atttaaaaat
     4261 gctctcttgt gttggtgggt cagaatagta tggtactcgt atttgagact aatattggca
     4321 aacaacataa agaaaaatat aaatttttta taataaactg tataaaattt taa
//
LOCUS       DAEMHZ010000213         4372 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000213 DAEMHZ010000000
VERSION     DAEMHZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.215"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(66..485)
                     /locus_tag="KPK72_003659"
     CDS             complement(66..485)
                     /locus_tag="KPK72_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG2490826.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(752..1534)
                     /locus_tag="KPK72_003660"
     CDS             complement(752..1534)
                     /locus_tag="KPK72_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2490827.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            complement(1599..2360)
                     /locus_tag="KPK72_003661"
     CDS             complement(1599..2360)
                     /locus_tag="KPK72_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2490828.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINSH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(3409..4221)
                     /locus_tag="KPK72_003662"
     CDS             complement(3409..4221)
                     /locus_tag="KPK72_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2490829.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
BASE COUNT         1463 a          739 c          412 g         1758 t
ORIGIN      
        1 gtgtataaaa ttaaaaattt tatacacctt tttctagcaa ttattaaaaa gatatttaat
       61 taaaattaat gacccttatg tttagctttt ctcttttctt gtttcaaatt aaatagtctt
      121 tctttttctt cttcttttct atttttagct aggttttttc tatcctgttt tctttcttca
      181 tattgaagct taagagcatt ttgagcctta gtccctatag tttctttttc ttgttccttt
      241 tttacttttc tctgcattct tttgtatcca atgttgtctt tggtcacatt tttttctaat
      301 ttaatgctac caaaatctaa tgagaaaaat ctttcaagta taaattcata cacttctact
      361 tctttaggtt ctgctccaaa aactacccta catgcttcat attttttacc atcttgtctt
      421 tcaaatatac ctacccaaaa tggttcttca aataagactg ttaattttcc actaattttg
      481 tccattgcta gttcctcctt aaattacttt ctaaagaacg gacaagcaag gaggcaggtt
      541 actgacagct attgctgcgt ctggactacc aaccagactg tgtttttatc tttatagatt
      601 aaatattatc ttttttaatc atgcttgtca atcttagtat caacatctat catattgatt
      661 taagtgaaat caagtttttt aactcgactt tattattgtt aacactaaga aaattaatta
      721 tttttcataa taaattagtt attatactta tttaaatatc aatctttatt gttccctgca
      781 taaacaagac tgcttcacca gaaattttca cagtattatc cttcaacact tcacactgaa
      841 ttataccacc acgattagaa agttgagcag ctaccatttt gtgttttccc aacttttcag
      901 accaatatgg aataagatta caatgtgatg aaccagtaac agggtcttct gaatctaatt
      961 ctggacaaaa atatcttgat acaaaatctg tattacttcc ttgtgctgtg attattatac
     1021 ctaaccagtc tgtcaattta cgcaattgtg cgtaattagg tttataattt ataacttctt
     1081 gttcactgtt tagcaataag attaaatctc ttgaagaata aacagcactt ggtacacagc
     1141 ctatcaaatt agcttgttgt ggtgataact ctatttcaat aggcattatt tctggaaata
     1201 tcatttcata tagatttcca tttcgtgtaa cttcaagctt tccactttga gtaaagaaat
     1261 ctatcctttt tacattaaca tcaataaaat ttgatattac ataagctgct gctaaagttg
     1321 catgaccaca taaatcaatt tctgctttag gagtaaacca tcgtaattca taatttcctt
     1381 tacctttaac tacaaatgct gtttctggta aattgttttc ttctgctatt ttctgcatta
     1441 actctaaagg tattcttctg tcaagaacac aaactccagc aggatttcct ttaaatagtt
     1501 tagttgcaaa tgaatcaact atgtagtatt ccattattta acctctcttt ctaacataaa
     1561 atatacatca atttacattc aataaaactt taaatttctt acatatattt tttaatatta
     1621 ctctgttcaa caactttttt acctaacaat gtaattgctt ttttggcaca attatttaca
     1681 caacgataac acattgtaca ttgcccattt gcaacaatca tacaattttt aattactaga
     1741 ttcttcattg gacataaatt aacacattta ccacatacaa tacattcctc agtattaatt
     1801 tttagtttat ctgtatacat atttgtttta ttgataaaat acaatcgttg tccaaataac
     1861 cctacaatat gactcaaaaa acccaaccct tcttttggtg gtgacccatg tttcagtttt
     1921 ttagcagcct tagttacttt cttttctgct tttattacaa gctttttatt gtactctaaa
     1981 gaatgtttta ataccttttc atctcctata ctatctggca tcttcacatg taatccacca
     2041 acagttatag caccgtattt ttttaatatt ctagataaca caccagagcc atctccacta
     2101 aatagagcca tagttgcaat aataaaaaca cgtttaccat tccacaaagt tgaatgattt
     2161 attataaaat ctttcaatat ttttggcaca ttactatatt gcacaggata actaaaaata
     2221 atattctcat gactattgat gtgctgtaaa agttcattat cttcaataga ataagaattt
     2281 gctgttatat catattcttg taaaaatttt tctacacagt atcttgaatt tcctgttcca
     2341 ctaaaatata ttcctatcat agtacttctc ctaatagcta agattatata gctctattct
     2401 taaaattctg actttttata ttatctaaat taagtagttt atttaaataa tataaactta
     2461 attcaacaag ttacctactg aacctcccat gactaaagtc atagggttcc tgcttgatag
     2521 aattttacat aatcaattta ctaaatactt gtctaattct ttttttatca ttttaaatat
     2581 acattttaat aatattagta agctatactt ccttaagcat atattttccc taaagtaaaa
     2641 ctattgatga ttattccatt atataaccta attataaatt tagtaagttc atcacatact
     2701 atacccttaa ttctcaatta taaagttccc taaaaaatat ttgaaaattt aactatgaaa
     2761 agatttccta ttgcctaata tgtataagta aggagtacta tatagtactc cttatattgt
     2821 actcttataa cattgatacc ttaatattat tttaatctta atcattattg atttgattta
     2881 aaattataaa taggttccaa tattttaatt atgtcaacta tatctcttat agttttaata
     2941 atctcatcaa ttggtttatg tgcttgttgc actttatcaa tagtattaat taaatccatt
     3001 tacacatcta gcacctgtat ggcaatttag cattatcctt atcttatact ttgttgtaaa
     3061 ttcttgatta cataattcta ttatctaact tatagctgtg caatctacat tatctgtaaa
     3121 tatctttgct ttatatattt tccctgaacc tctaacatta atataacagc ctttttgcta
     3181 tacactttta actatatact taattttaat aacattactt gtttataaaa atatactata
     3241 gataaatttc acataaaaat aataattcaa gtattgattt ttatataaga aaatttgtaa
     3301 tgtactaaaa ataacataaa agtttccatt tttcaatatg taagcttttc ttatgttatt
     3361 ttattgttaa aggtttcaag tttttattta ttcattttaa aaaaattatt atttttgtaa
     3421 aactaatccc atagtcatac tatcgtcaga atatgcttca cccttaacat tactaaaaaa
     3481 ctcaacagac ttaaatccaa tattttcaac ttctaataag aaactttctt ttgaaaaata
     3541 gtgattccaa acataaaata cattttcatc tttttcggtt attacaagag tttgttctaa
     3601 tgtattataa tcattgtatt tacaattatc ttgtagacac atatattttt cattactcca
     3661 aaatcctcca tcttcattta tttcccaata tttaatttcc tcaaaattat tgtatttatt
     3721 tattgtaaat acatctaata ttaattttcc tccaggtttt aaactatcat aaattttttc
     3781 cattaataac cttctatttt cagttgacaa agctccataa tcacaataaa ttaaagtagc
     3841 caaatcaaat tcatcattat agttcatatt taaataactt tgaaacaagt aatttatgtt
     3901 taaattagtc tccttatttc tattttgtgc atagttaata gaacgttttg agaaatcaat
     3961 tccagttacc ttataacctt tttgtgcaaa tttctctgca tataaacctg gaccacaacc
     4021 taaatcaagt aatttaggat aattaactgg atttgcaact gtaactatcc aatttacaga
     4081 atcctcgata aagttaaaat ttctacttgc tccttcaaaa ttagtgtcta aatgtgcttt
     4141 taataattgt tttgaaatat actcatcatc ccaaaaatta atttcacttt gtttataaag
     4201 ctctggtctt tctaaataca tacataattt gtttaacaaa acataccact ccttaaaatt
     4261 tatttttaaa ataaaaaaac cacagagttt actctgtggt ttaacttcaa aaagatgagt
     4321 ttaaataaga gtaaaataga accaaatatt tttaagcaca acaataaaac ta
//
LOCUS       DAEMHZ010000214         4288 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000214 DAEMHZ010000000
VERSION     DAEMHZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.216"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..468
                     /locus_tag="KPK72_003663"
     CDS             <1..468
                     /locus_tag="KPK72_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2490830.1"
                     /translation="LGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEI
                     AKDMKELGYTFVATEGTAKSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPT
                     KGNDSTRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            726..1775
                     /gene="carA"
                     /locus_tag="KPK72_003664"
     CDS             726..1775
                     /gene="carA"
                     /locus_tag="KPK72_003664"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2490831.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1968..>4288
                     /gene="carB"
                     /locus_tag="KPK72_003665"
     CDS             1968..>4288
                     /gene="carB"
                     /locus_tag="KPK72_003665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG2490832.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         1772 a          433 c          858 g         1225 t
ORIGIN      
        1 ctaggtgtag gagaaaattt agaagaagcc ttatataaag ggttcttggc agcaggtaga
       61 catatgtctg atgaaagagg agtagtactt gctactgtaa ataatcatga taaagatgag
      121 tttatagaga tagcaaaaga tatgaaagaa ttaggatata cttttgttgc tacagaagga
      181 acagcgaaga gtttaagaga aaatggaata gaggctgata tagtaaatag agtagaagaa
      241 tctagaccaa acatattaga tgctataaga aataaacaag ttgatatagt aataaataca
      301 ccaactaaag gaaatgactc tacaagagat ggatttaaga taagaagaac agctattgaa
      361 ttttctactg aaataatgac atctttggat acactaaaag ctttagtaga agttaagaag
      421 aaacatttaa ataaagatga gttaaaagtt tacaatatag ctgaatagaa attaagttga
      481 ttatatagtt taagaattta attattaaat aaaaaaaaag aggttatttt ttctaggagt
      541 ttttactttt gagataactt ctttttttta agaaatttgt agtataatag aaaaagataa
      601 aaaataaatg taaataagta taaaactaga atgtaaaagt gaaaaataga tatagatatt
      661 taaaagctta tatttagtta atgttcttta gtttgcctaa agagaattat aggaggtact
      721 gtagtatgaa agcaaggtta atattagaag atggaacagt ttttatagga aaggcatttg
      781 gatatttaga agaaagtgta ggagaagtag tatttaatac ttcgatgata ggatatggtg
      841 aagttttaac agacccttct tactatggtc aaatagttac tatgacttat cctcttgtag
      901 gaaattatgg tataaattta tcatcagcag agtctgaaaa agttcaagta aagggattca
      961 tagtaaggga gaaaagtgat tctccaagta attttagatg tgaaattgat atagaccaat
     1021 atttaaagca aaataaagtt atcggacttg aaggaataga tacaagagct ttgactaaga
     1081 tacttagaaa taatggaaca atgaaaggta taattacatt agaagacagt aaattagaag
     1141 atgttaaaca taaattggat aagttttcaa atacagaagc agttagaact gttacaagaa
     1201 aagaagtaga gcatataaaa ggaaatggtc caaaagtggc tgttatggat tttggagtaa
     1261 aaagaaatat attaagatca tttatagctc gtggatgtga tataacaata tttccagcta
     1321 caacatctcc agaagatgta ttgagtataa accctgactt aatattcttg tcaaatggac
     1381 ctggagaccc agaagaccta gaagatgtta tagaaaatat aaaagctctt attggtaaaa
     1441 aacctatagt tggaatatgc ttaggacatc aacttttagc tttagctctt ggaggaaaaa
     1501 ctgctaaact taaatttggg catagaggtg gaaatcatcc agttaaagat ttagaagaag
     1561 gaaaagtatt tataacttct caaaatcatg gttattatgt ttcagaagtt cctgaacaaa
     1621 tgaaagtgac acatataaat ttaaatgata atactgttga aggaatgaga catgagaagc
     1681 tagatgtata cagtgttcaa taccatcctg aagcttgccc aggaccaaaa gataatgact
     1741 atatttttga taaattttta gaattagtaa aataaacaac attagaatag ctaactaaaa
     1801 aacaagaata acttaaatca aaaagtttgg tttaattaac atagatgtat taaatagatg
     1861 tattgatgag taaatttgaa cttaagataa aataaagtaa ttaaagttga ttgtatagat
     1921 gatgaagata agaactgtga aaattgttta gataaggaga aagagttatg cctaaattag
     1981 atagtataaa gaaaacttta gtattagggt cagggccaat aataataggt caagctgcag
     2041 agtttgacta ttcaggaaca caggcctgcc aagcattaaa agaagaggga attgaagttg
     2101 tattagtaaa tagtaaccca gctactataa tgactgataa agaaattgca gataaaatat
     2161 atatagaacc attaacaata gaatttatag aaaaaataat agaaaaagaa agaccagaca
     2221 gtttacttgc aggtatgggt ggtcagacag gattaaactt agctgtagaa ttacatgatg
     2281 ctggtatatt agataaatat aatgtaaaag ttataggaac atctatagaa tctataaaaa
     2341 agggcgaaga tagagattta tttagagaag taatgaaaga aataaatcaa ccagtaatag
     2401 ttagtgatat agttacaaat ttagaagcag gtcttgagtt tgccaataag ataggatatc
     2461 cagtagttgt aagaccagca tatacgctag gtggaactgg tggaggtata gctgatactg
     2521 aggaagaatt aagagaaata ctttcacatg gactacagct aagtcctgtt ggacaagttc
     2581 ttcttgaaaa gagtataaaa ggttggaaag aaatagaata tgaagtaatg agagatggaa
     2641 atggaaactg tataactgta tgtaatatgg aaaatgtaga cccagttgga gttcatacgg
     2701 gagacagtat agttgttgct ccaagtcaaa ctctaagtga tgaagagtat caactactta
     2761 gaaaggcatc aatagacata ataaatgcaa tagaggttca aggtggatgt aacgttcaaa
     2821 tagctttaaa cccacatagt ttagaatatg cgataataga aataaaccca agagttagta
     2881 ggtcatcagc cctagcatca aaagctacag gatacccaat agcaaaagta gcagctaaga
     2941 tagctttagg ttatactttg gatgagatag aaaatgcagt aactaaaaaa acttatgctt
     3001 gttttgagcc aacattagat tatgtggtag taaaaatacc aaagtggcca tttgataaat
     3061 ttagacatgc caatagaaaa ttaggaacta agatgatggc aactggtgaa atcatgagta
     3121 taggaagtaa ctttgaggct gctattctta aaggtataag atcacttgaa acaggaaaat
     3181 attcattggt tcatgctcca tcagaaggta gaacattaga agaattaaag gctagagtag
     3241 tagtacctga tgatgagaga ctatttgact tagctgaaat gataagaaga ggctataaag
     3301 tagaaatgat atcagaaata actggtgttg ataagtggtt tataaataaa tttaaatgga
     3361 tagtagaaca agaagaaaag ttaaaagttc taaaaataga agacttagat aaagaatatt
     3421 tacatgaatt aaagaaaaag ggattttctg ataaaggtat atctgactta atgaagataa
     3481 gcccagaaaa attatatgaa ttgagaagtt tatataatat acaaccagtt tataaaatgg
     3541 ttgatacatg tggaggagaa tttgaagctc tatcacctta ttactattca acttatgaac
     3601 aatatgatga ggtagtagtt agtgataaga gaaaagttgt agtattgggt tctggtccaa
     3661 taagaatagg tcaaggaata gaatttgact attgttcagt tcactgtgta aaatctttaa
     3721 gaaaaatggg aatagaaact ataattgtaa acaataaccc tgaaactgta agtacggatt
     3781 ttgatacatc tgataagttg tattttgaac cattaacaga agaagaagtt ttaaatataa
     3841 tagaaaaaga aaaaccagaa ggtgtaatac tacagtttgg tggacaaaca gctataaaat
     3901 tggctaagtt tttacatgaa aaaaatatac ctatactggg aacagacttt agagatatag
     3961 atgcagcaga agatagagaa aaatttgatg atttactaga gagactagat ataaatagac
     4021 caaaaggaaa aggtgtatgg acaactaatg aaggtgttga aattgctaag gagttaggtt
     4081 atccagtatt agttagacca tcttatgtac ttggtggtca aggtatggaa ataacttata
     4141 acgaagaaaa actaattcag tatttggatg atgcatttga tagagaccat aaaaatccag
     4201 tacttataga taaatatcta actggtagag aaatagaagt agatgctata tgtgataaag
     4261 aagatatatt gatacctggt ataatgga
//
LOCUS       DAEMHZ010000215         4286 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000215 DAEMHZ010000000
VERSION     DAEMHZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.217"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(255..1412)
                     /locus_tag="KPK72_003666"
     CDS             complement(255..1412)
                     /locus_tag="KPK72_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG2490833.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1566..2135
                     /locus_tag="KPK72_003667"
     CDS             1566..2135
                     /locus_tag="KPK72_003667"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG2490834.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(2317..2733)
                     /locus_tag="KPK72_003668"
     CDS             complement(2317..2733)
                     /locus_tag="KPK72_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490835.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2959..4053)
                     /locus_tag="KPK72_003669"
     CDS             complement(2959..4053)
                     /locus_tag="KPK72_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490836.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         1483 a          759 c          456 g         1588 t
ORIGIN      
        1 attttaaaag taaatgatat aatatttgga acaactgggt tgcaatgtgt ttttaaaaaa
       61 tatcaaaata aggctatgat ttttaaaacc atagccctgt ctgtaaaaat ttaattatca
      121 cttaaaaaat aaatttttat ttaaagaata cacataaata tttccagact ccctcatgaa
      181 ctaaaaagtt cattttgaga catctctttt ataatgttct ttattttata gtataagttt
      241 ataaaaatta aatcctattt atcaatctgt tctctaagcc acttagttgc tacatctaac
      301 tgtttatttt catctttctc ttcaatagta ggctttatac ctttttcatt gatataatcc
      361 ccattaggtg taaaatattg agcagtagtt agcttgtatc ctgttccatc cttcaatctt
      421 acaactgatt gtaccaaccc tttaccaaaa gttgtagtcc ctacagaaat accttcttta
      481 ttatcaacga ttgcagctgt taatatttca gcagcagaag cactttctcc attagttaac
      541 actactattg gaagtcccaa tttttcttta tcagatttca agtactcagt atttccctta
      601 ttatcttttg tatatactat agttccttca ccaattaact catcagcaac ttctttacat
      661 atatctagca ttccaccagg attatttcta aggtctatta ctaatccctt gatatttttt
      721 gcttttaatt cttttaaagc tttatcaaag tcagtatatg tacttgatat aaattgggta
      781 atttctatat atcctaaatc attatcaatt actttacttt ctatactctt ctcaattatc
      841 tggtctgttt ttactttaaa gttcaattgt ttatcttctc ttaatatagt cagttcaact
      901 gttttacctt ttttaccctt catcatacga acagcttcat ctgaattttt atatgagaca
      961 gattttccat tgacttttac taatttatcc cctgatttta cacctgcttt ctctgctgga
     1021 gaatctttca tgggaactac tactaagcca ccatcttttg atgcccctat atacatacct
     1081 acaccaacat aagaaccttc actatcatta attagattct ccatttcatc tttagtataa
     1141 taaccagaat acttatcatt tgttcctaga aatagacctt ttgctgctcc atttaccaaa
     1201 tcagtatctg atactttttt ataaaaatct tgttttataa tagattctag tcctaacaac
     1261 ttatcatatt ttttatagtc ttcatagagt tctttcgata ttacaacttt attccccaat
     1321 gtcaactgaa atgctgatgt aaccatagct gttataatta ctaaaatcac ccctaaaaaa
     1381 atagctttct tctttgaaat cacaagatac atctcctttt acataaacta ttattattat
     1441 atgactagct gttattttta ttacatttac ccaaatatat tgctttatta caataaatta
     1501 tactaaaatt tatattaaat acaattgctg tacacattat aacatattaa gggaggaatt
     1561 attttatgaa aaaagaattt agaaaaaaag ttattgaatc aagaaaaaat aagagtgcaa
     1621 acttcgtact tagtaattca aatttaatca ctgaaaaatt acttcaaatg gaagatataa
     1681 gaaatgcaac taatataatg ctttatctaa attttaacaa tgaagttcaa acagataatt
     1741 taatcaaaaa acttttgtct ctaaagaaaa tagtatcaag tcccatcaca ataaaagaaa
     1801 atcatacatt aatccctact caaataacag atttaaaaga gggattgaga attggagcat
     1861 atggaataag agaacctaac gaaaaatcac ctacaattga tattaaggcc ttagatgttg
     1921 taattgtacc agctgtagca tatgatatac attgctatag attaggatat ggtggaggat
     1981 tttatgatag atttttagaa aacttaagaa aagatgctat aactatcggc atagcatttg
     2041 acttccagat attcgattct attccaaaag aaactcatga tgcacaatta gactacatag
     2101 ttactgaaac tagaattatc actccaaata attagaatat taagtatttc cagactccat
     2161 ttaatgtagt aataaatact actgttaata tcttacttta taagcatttt aagtcatggc
     2221 ttttacaaca ataagaacca tatacaattt acttacatta ataaaaacca tgactttatt
     2281 gtacaatatg cagttattta attataaaat accttcttat ttcgtcgcat ttaattctat
     2341 tctatctgga agttcactca tatttgtttc aacaactatg tatggaaaag aatcttcctt
     2401 atctttatac tttatcttga ctctcaaaac atcctttcct tcctcaacca cactttctat
     2461 tttgtccatt tctataccat atttattatt tcctctagtc actataatgt atattttgtt
     2521 atcgatttta gccgctaatg ctctttcttc atttgtatac ttatccatca catctaatat
     2581 cttttctggt attgaatttc tttgtaatac tgtataatct atgggtttag atttctctac
     2641 aaacatactt ggtatgaata taaatccccc tataacgaat acaagagcta taactccata
     2701 aattatacct ttaatattaa attttacatt caatcttatc actcctccca tatattctaa
     2761 atatatgatt tataagctta tcttagactc taaattattg attagtatat atttatccat
     2821 aattataaat atatgtaatg taactaaaaa acatgttttt cctatttcat aataaacgag
     2881 ctaaaaaata aaaagagcta tatgaaaaca ttttcattgt tttcatatag ctcttgagtt
     2941 tacaatatta aaaaatactt atttgtcact gattccttct tgatatccag atgtttttgt
     3001 aatctgtcta taaagaacac aaaccagtat tatgacgccc atatatccag tatatggata
     3061 taaaatacca accagcttat caaatggtag gaagccacct ataagaccta atatagctgc
     3121 tattaaagtt attaatttaa acttaggatg tttatcatct acaaatctat tagttactga
     3181 ccataataaa ggtacagctg ttgtatatat acctaatata agaattattg agaataaaac
     3241 tccaattact ggtgaaatct tatctgctaa gaataatgat ggtattgctt ttgaatataa
     3301 gtttcctata tcagaaagaa tagctaagtg catcattata gctgcagcca taagtgctac
     3361 tccacctaat ataccaccac aaatagcttc ttttttgtta gcagcacttg ctcctaatcc
     3421 agttaagaat gccataacaa caattgtgtt ataagtagta tatattacac cagatagata
     3481 ggcatttgga actggtttag ctatctttac agtgctaaga acttgtgcag cttctcctat
     3541 attacctatg tttttagcta aacttatacc accaactaaa agtgtaaaga ttattattat
     3601 aggccctatt ccacctagta tctttgataa tttatcaaga cctagtgaaa cagttaataa
     3661 tgctactact gccataccta ctctacctac aaatggattt aatccatagt attcagttaa
     3721 agttgctcct gctcctgaaa tcattactac gaaaacacca aataagaata aaggtccaaa
     3781 ccattcaaag aatattccta aatacttacc acaataaaca cgataaattt tgataggttc
     3841 tttaagttga agctctttac cttttgacat aactgaagca cccatccatg agaataatac
     3901 cattgaaatt atcccaccaa taatacctgc aaaaccataa aaagagaaga attgcattat
     3961 ttcctgtcct gttgcaaatc cagaaccgat tactgttgct acatacgcac ctgcgaaact
     4021 gatgactgtt ttaacattta ctttttctga catatctcag ttacctcccc atgtgaaatt
     4081 ttttcttctg attaaacaat tcgataaact acctacaatt tcctttaaca taaaatacat
     4141 gataactgtt ttatagcttt ttagtataat aacttaaaac ttataatctg attttatacc
     4201 ctactcctcc tctcataatt tttttagatt cttttatcta agtagtcatt atatataaaa
     4261 ttagtttctc tataaattat atatta
//
LOCUS       DAEMHZ010000216         4238 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000216 DAEMHZ010000000
VERSION     DAEMHZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.218"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(184..1227)
                     /locus_tag="KPK72_003670"
     CDS             complement(184..1227)
                     /locus_tag="KPK72_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG2490837.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1248..1694)
                     /locus_tag="KPK72_003671"
     CDS             complement(1248..1694)
                     /locus_tag="KPK72_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG2490838.1"
                     /translation="MKLIWLIVAILFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1872..3452)
                     /locus_tag="KPK72_003672"
     CDS             complement(1872..3452)
                     /locus_tag="KPK72_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG2490839.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIEIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYMVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         1375 a          749 c          396 g         1718 t
ORIGIN      
        1 tagtaaagga taaattgtat aaaaattctg ttaatttatt tttataattt catctttcta
       61 ataaaaacaa agaagaagac ttaaaaattt ataagtcctc ttcttttcaa cgtgcatttt
      121 tgtgctatat ttaattaatt cgacctttaa tttccatatc cttcctatga taacaaaact
      181 cttttatttt tttaatgatt cattatcatt taaaacttct tttatatcaa gtacttcttt
      241 gttatcatca ctcataactt cctttattcc tttgaatgtt cctaggaaat taactgcttc
      301 tgaaggtaaa accagttttg ttgacttacc ttcagccatt ttttcaagag cttccataga
      361 ttttaatgct agaatattat tgtctatatc agcctctttc attgctgaaa aaactaattt
      421 taaaccttct gctgttgcca tttgagttct ttttatcatt tcagcttcac cttgtgcctt
      481 agcaatagct gtctgtctta tagcttcagc ttcaccttct gccactaata tagcagattc
      541 tttttcacct tctgctacac gtaccatagc ttcttttttg gcttctgctg ttaatattgc
      601 agattgtttt tcaccttctg cttgaagtat tgcagctgac ttattacctt ctgcttgaag
      661 tattgcctct cttctttctc tttctgctct catttgtttt tccattgcaa cttgaatatc
      721 ctgaggaggc attatatttt ttaactctac tctatttact tttatacccc atttatctgt
      781 tgcttcatca aggatagttc tcatttttac atttattata tctcttgatg ttaatgtttc
      841 atctaaatca agttcaccaa ttatatttct tagtgttgta gctgttaaat tttcaatagc
      901 agctattgga ttagctatct caaacacata tctaactggg tctgtaactt tataatatac
      961 aactgtatca atctgcatag ttacattatc tttagttata actggttgag gtggaaaatc
     1021 tattacaatt tctctaaggt caattacata tgccatttta tctaaaaatg gtattaagaa
     1081 atgtacacct gtttcagcca ctttttgaaa cttaccaagt ctcattatta tacctacttt
     1141 agattgtttt ataactctta tacaagttaa acttattgct acgactacta ctattaatac
     1201 aatacttaaa acaattttag tacccatact ataaatcctc ctttattcta ttggtttgtc
     1261 acattaactg tggcgtctac ttttttaaca actaatttta ccccttcaat ttttaatact
     1321 tctactgtag aacctttttc tatcttttca ccatctatag aaatagccgt ccatgtttca
     1381 tgttcaacta caactagacc tgtgttattt ggagaaatct cttctgtaac aatacctttt
     1441 ttagacatca ttgcttgtaa attagtgtta gatttatagc ctttatcttt ttttactatt
     1501 tttttagtag ctacgactaa cattgctata gaaattactg caaatattaa aatttgtaag
     1561 aaaatactct ctataaagct acttaaaaat ataagtatta cagctcctac actaaaccat
     1621 atcaatgtta agcttggtgt taacatttct gctattccaa ataagattgc tactatcaac
     1681 catattaact tcatactatc taccctcttt tcatatgaat tataaaaccc tatataaatt
     1741 atatgtttat acaatgtttt tgaatataaa tttaaattac aaaacaataa aataaataaa
     1801 ttaattaatt aatagtatta tatcaacata ctttgttaaa attgttacaa agtagtatca
     1861 aatatcacca attatatatc caacaactct ttcattgtta atgaaatgtt acttattaaa
     1921 tccgtagcat ttaccatata cattgctttg ctcaatttat ctcctatata gccatgtata
     1981 tatgctccac taactgtagc ttccaatata tccatacctt gacctataaa tgaagtaatc
     2041 attccaagca aacaatcacc cattccacca gttgccatac agctatttcc tgtaggattt
     2101 atatatgttt ttttaccatc tgttatgaca gtttgataac cttttaaaag caccaccaga
     2161 ttatattttt gagcaaattc ttttgcaaca tctactctat gttctcttat atacccaatt
     2221 gaatctcctg tcaacctggc catttctccc aaatgaggtg taattacaaa tctatttttc
     2281 cattctaaaa acttataaca tctgtccttc ataacgttca atccatctgc atctaatact
     2341 attgggcaat tagaattttc tactatctta agtaatttat caaatgtttg actattatct
     2401 cccatacctg gaccaaaacc aattgcatca ctagaattaa gtaatttatt taatctttct
     2461 tcttcaaaat ttaaagtcat tgcttctgta agtctaatag ataccttttc ttgcacatct
     2521 tcactactaa ttaatgttac aagtccactc ccactcttaa cagcagattc agttgctatg
     2581 aaacttgcac cataaaatcc ttttgaacct gcaactatag atacttttcc aaaatcactt
     2641 ttaaaaccat gcttatctct tcttctaata ttatttttta caaaagtctt atcagttatg
     2701 aactcttcat catggtattt tcttgtaata aaatctggaa cacctatctt ttctacaact
     2761 acatcaccta taagtgtgct agtgtcatag tttagaaaac ctcttttata aaactcaaag
     2821 ctaatcgttt tgtttgctct aatacaagtt cctaatacat ttccattatc gctatctata
     2881 cctgaaggaa tatctataga atatgtattt ttgctattat cgtttactat ttcaataact
     2941 tctttaaaaa taccagttat atcacgtttt aatccagtcc caaatatagc atctactaca
     3001 acatcacttt tagatactct atctttaaaa attgttaaat ttttattttt tataatattt
     3061 tgtttctcaa ctttaataat tttatctact tcattttcat ctccataatt tttttcactt
     3121 aaatcatttt tattaatatt tataatatta attcccatat ttaaaagtac attgtaattt
     3181 attttagagc attcacttag tttttcaaga ttacctacta aaaatacgtt tacttctttt
     3241 ccatatgtat ttagatgtct tgctattcca agaccatctc ctccattatt tccaacacca
     3301 catacaactg tataactatt atacatgtct atatccatat ttttcattgc agatataacc
     3361 gcattttcca tcaatacaat cattggaatc cctacttctt ctacacatgc agtatcaaca
     3421 tttctgctta atttagatgt acctattagc ataaattatc actcctataa agttaaagac
     3481 ttcttaaata cttaccaaaa atatctattg aaatcttttt aatcccataa atatcataaa
     3541 taaaatcact aaaactaaga gttttgaaat aatttaaaat aacaaaaaat tttgaattaa
     3601 aacaattatt taatttatta taccatataa tttaagttat tatgttgatt taaaacctat
     3661 ttacactaat tatctaaata tctctttatt tttataatct tttatgactt atttatattt
     3721 ttcattttta taaaaaaaca ataattaaga atataactat attctacatg taaaaatttt
     3781 tttatttaaa ctcaattttc tgataatttg ataatttttc aaaatttata gttgactttt
     3841 attttttact gtattaaact aattactata caaaacagaa aacaaaattt tcaaaattat
     3901 taaattaaaa ggagaggtaa atatgaagac tttattaaac aaacaagtac gattttttag
     3961 tagccaattt agttagggtt tgtacctaag ttaagacaaa ttgtcataac atagatacaa
     4021 gccccttagg attatgattt ttaaggattg tatctatgtt ggctatagta attgttgtta
     4081 aagtttttat tttatcatca taatatagag ccacatggat tatactgtgt ggcttattcg
     4141 aatttttgcc atacagttat atatactgtg tggttttttt atatcaaaat cattttttaa
     4201 aaccaaattt aaaaaaccat ttaaaatatt ttcaaagg
//
LOCUS       DAEMHZ010000217         4212 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000217 DAEMHZ010000000
VERSION     DAEMHZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.219"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            20..116
                     /locus_tag="KPK72_003673"
     tRNA            20..116
                     /locus_tag="KPK72_003673"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54..56,aa:Sec,seq:tca)
     gene            266..973
                     /locus_tag="KPK72_003674"
     CDS             266..973
                     /locus_tag="KPK72_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG2490840.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(1115..1951)
                     /locus_tag="KPK72_003675"
     CDS             complement(1115..1951)
                     /locus_tag="KPK72_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2490841.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            2139..3044
                     /locus_tag="KPK72_003676"
     CDS             2139..3044
                     /locus_tag="KPK72_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG2490842.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            3053..>4212
                     /locus_tag="KPK72_003677"
     CDS             3053..>4212
                     /locus_tag="KPK72_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490843.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKK"
BASE COUNT         1642 a          409 c          682 g         1479 t
ORIGIN      
        1 ttgagaagga tttcattatg ggagtggatg ggtgccggtg tgccctctag tcttcaaaac
       61 tagtatgagg ggttaagagc ctcttaggtg ggttcgattc ccacgcattc ccgccaatat
      121 gtaaaaggta tcttttaatt aaaagatacc tttttaaatt tgttttaaga taatttagtt
      181 ttaatgatat ataatatttt gtattatata atgtaaattt tataatacta ttattgttca
      241 gataaaagtc taggagagtg aaaagatgga aaataatttt acaacgagaa ctagttttct
      301 agttggagat gatgggatag aaaaacttaa taattcaaat ataatagtat ttggagttgg
      361 aggagttggt agttttactg ttgaagcttt agcaagagct ggagtaggca atattactat
      421 tgtagatttt gatgatgtag atataaccaa tataaataga caaataccag ccctacattc
      481 aactgtagga agatacaaag ttgatgtcat ggaagaaaga atattagata taaatcctaa
      541 tatcaatata aaaaaaataa gaagtcttta taataaagat actagtgacg aaatactaac
      601 tgaaagatat gattatgttg tggatgcgat agacatggta agttccaaaa tacatctaat
      661 tgaaacttgt gaaaaaaaag gattgaaaat aataagttct atgggtatgg gaaataaatt
      721 agacccaact aaaatagtcg ttacagatat acacaaaaca agtacttgtc ctctagctaa
      781 agttatgagg aaagaactta gggatagagg tatcaaaaag ttaaaagtag tatattctac
      841 tgaacaacca atagaactta agaagaaagt aatgaatggt agaaaagtaa cacctggtag
      901 tgtatcattt gtcccatctg taggaggttt aataatcgca tctgtaatag taaatgaact
      961 attaggacaa taagttttaa atattttaat ttataaattt tatatacaaa tttgaaggct
     1021 agaagcatga atataaagca attttatatt catactttta gcctaattat tttatattac
     1081 cttattttat gtagttgtag ttttattttt tagattatgt atctatttca taattatatt
     1141 ttgtaaatat aatttctcca tcactataaa aaacaatatc tcctattcta tctgttctat
     1201 aaattgatac atggttttgt tttagattat taataacctc cctatgtgga tgaccatatt
     1261 gatttttata tccacatgat attgcagcta tatctggact aatttttttt ataaattcta
     1321 aagatgaaga agaattactt ccatgatgac ctatttttat aaaattaata ttatttaatt
     1381 taaaagaatg taaaatttct ttttcatttt cttcttcagc atcacccata aataaaaaag
     1441 aattatcatt aaaagttaat ttgaaaacta tagaatttaa attagattct tcttgtatat
     1501 atgatggact taatacatat atatttatat tgttatctat gtttaaaaca tcatttttat
     1561 agagatgctg aatagataag tttttatccg tacaagaatt tattagattt tgataagcct
     1621 cactatcagt tgattgttca ggcatatata tggagtttac attaaatttt tttataatat
     1681 tatcaagact tcctatgtgg tctgaatctg gatgagttgc tattataata tcaatagttt
     1741 ttattctttt ttgtctaaga taactaataa ttatattttc gctatcttca tcacctccat
     1801 caattaatat attcttattt gtgggtgttt gaactaaaat actatcacct tgtccaacat
     1861 caatcatatg tatggatagt agagattttt tgtcacagcc gattaaaagt gatattataa
     1921 taagtattga taaaaaaatt ttatttctca ataaaaaatc actccattca ttacatgttt
     1981 atataaattt atgatgggta atataagata tatatgactt attatatttt gtattttaaa
     2041 caatataaat gaaattaata ccataataaa attggagttt tttatatata caaaataaaa
     2101 tttttaaaat taagcaataa aaaaggtagg aggattttat gtttttcaaa gatattgaac
     2161 taaattcaaa aaaagagtta gacccatatt ttgatttagt agattatgaa gcttgtgagt
     2221 actgttttag tactttatat atgtggcaac atgtttataa gacaggatat tatataggtg
     2281 aagattttgc cgttttagtt ggggaatacg aaggagatag cttctcaata ttaccacttg
     2341 ctaaaaaaga taaacttcca gaagttgtag attttgtcct tgaatatttt tctaaaaata
     2401 ataaaaaaat atacttaaga ggtattacta ctgaagtagt agagttttta aaagaaaaat
     2461 atccaggtag atttgagtat atagaagaaa gagatttatt tgactatatt tatgatgcag
     2521 aaagtttaag aactctagct ggtaaaaaga atcaaaagaa gagaaaccat ataaactatt
     2581 tcttaaaaga gtatgcaggt agatatgaag ctaaattatt agataaagaa aactttgatg
     2641 agtgtttagt actaatgaag gaatgggaaa gtaataagga agaaaataat gaatttgatg
     2701 aaagtatgga tgatgaatta ataggtatca aaaagatatt taaccattat gacatattaa
     2761 aagataaggt aaaagtgttt ggtgtttatg tagatggaaa attagaagct tttagtatag
     2821 gagagctttt aaatccaaat atggcattga ttcatataga aaaagctaat cccgatataa
     2881 gaggtctgta tccatttata aatcaacaat ttttggtaag tgaatttaag gacgttgagt
     2941 ttgtaaatag agaagaagat ttaggtattg aaggattaag aaaagctaaa ctttcttatc
     3001 atccttgcag atttgttgaa aagtacagtg ttagggaggc ttaatagact atatggagat
     3061 taggtatgct aaagaagaag agatagaaag tataaaagag atttggagtt attgttttaa
     3121 cgatacggag agttttatga agtactattt taatgataaa tataaatctg aaaatactgt
     3181 agtagcttta gatgaaggaa aaattatttc ttctcttcag ctaaatcagt ataagttatt
     3241 actaaatagc aaggtatata atacatcata tgtagtagga gtttctacac ttccagaggg
     3301 aagaggagct ggatatatga acaaagtaat gaaatttact ttaaacgaat tgtataaaaa
     3361 agggcaatta gtttctatat tgatgcctat tgactataga ctttatagaa gatttggata
     3421 tgagcattgt tacgaccaga ttgaatatac tattaacaca gatgatttaa aaaattttaa
     3481 gtcaagtgga aaaatgataa agtctaattt atcacaaatt gatgatttga tacaaataga
     3541 tagaactttt ttaaatgagg ttaatggtaa tgtgttaaaa gatgaacatt attatgaaaa
     3601 cttatttaaa gagattcaaa gtgaagatgg atttttatac attcatgaag gaaatgaaaa
     3661 agatggatat atagtttatt ttcttcagga agataaaatg tttgtcagag agttatttta
     3721 taaaaatata gatgccctta aatcaatgct taaatttata tataatcaca atactcaatg
     3781 taagatagtt actatatcta ctcctacaat agataagata agatttatat tagacaaccc
     3841 taaagatagt gatataaaaa taaaaccttt tatgatggga agagttataa atgtaaagaa
     3901 gtttattgaa gatatagata ttgagaatga tattaatagc tcatttaatt tattaataga
     3961 agacaagttt atagatgaaa ataatggtct atttaaaatt tctatacaga ataagaaggt
     4021 atctgtagag cagttagata aaaaaggtgc agaaaagcct caagaagatt ttgacattaa
     4081 attagatata aataccttaa cacagttatc attttcttat atagatgtaa atgaagctat
     4141 ttttttaaat gatataaaag atgtttcaga agaaacacta gaaacactta attgtatttt
     4201 ttctaaaaaa aa
//
LOCUS       DAEMHZ010000218         4200 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000218 DAEMHZ010000000
VERSION     DAEMHZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.220"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            705..3959
                     /locus_tag="KPK72_003678"
     CDS             705..3959
                     /locus_tag="KPK72_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBG2490844.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHAKATEGTAE
                     GEYAVGSKATYKTAIDEAQAILDKSDATQKEVNDALSALNTATETFEAGKVVAVDKTA
                     LQDAITAATALHNGATEGTSEGNYAVGSKATYKTAIDEAQAILDKSDATQKEVNDALS
                     ALNTATETFEAGKVVAVDKTALQDAVTAATALHAKATEGTAEGEYAVGSKATYKTAID
                     EAQAILDKSDATQKEVNDALSALNTATETFEAGKVVAVDKTALQDAVTTATALHNGAT
                     KGTSEGNYAVGSKATYKTAIDEAQAILDKTGATQKEIDDALSALNTATDTFKAGKVVL
                     NKTALQDAVTEATSLHAGATEGTAEGNYAVGSKATYKTAIDDAQAILDKTGATQKEID
                     DALSALNTATDTFKAGKVVLNKTALQDAVTEATSLHAGATEGIAAGNYAVGSKATYKT
                     AIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPTTIALMLSRILGFMK"
BASE COUNT         1880 a          539 c          738 g         1043 t
ORIGIN      
        1 tttaatataa ataatgtatt ttttgtagat gatttatgta agatttatgt tctttaatca
       61 taacaatatt atattgtaaa aaataaattt aataaaaaat gacttggaat ttatattcta
      121 ggtctttttt atataaaaat ttaataagtt tcaaatgttg gtaactttga cagtagaaaa
      181 cgtctattga gataacgagg attctaattg agggggagat atattagcac ttaaagtagt
      241 atatgcaaca gatataatct ttataaaagt aattcgaatt aaaatgtaaa tataaagaat
      301 aattaaaaaa atatagcaat tatttgtttt tcatatggat aaaattggtt atttaatata
      361 taattacaag tataaataat aaaatttatt tgtaaaaaga gtagcacttt tttaattcta
      421 aaggctactt tttttataca aaaccctatt ttttataaca attcattaac ataaaaatca
      481 aacttacctt aaaatataaa tttacaaatt tttcaatcaa taagaaaatt tctttattcc
      541 ttacataatg actgcaatgt aacaaaataa ttacaaatta attatgaaat cattatagtt
      601 agaaacctct ttaaaattag atattaagta tcctttagaa ttaagaacgg gaacaaagtt
      661 aaaaaattaa gtaatatcaa atggacaagg gggaagaaaa taaaatgaaa ataccaaaga
      721 agattgctgc aatgcttaca gttacaatga tagctggaag ttcaacagca ggcatagcta
      781 gtgcacaaac tgtggcaaca aatttaacag ggcaagaaag atatgaaact gctgttaaaa
      841 taagtcaaga tggatggaaa aatgctgatg aggtagtaat cgtaaacgac tcatctatag
      901 cagatgctct atctgcaaca ccatttgcaa aagcaaaaaa tgctccaata cttttaacaa
      961 gtaaagataa attaaatgat aagacgaaag cagaaataca aagattaaaa gctaaaaaag
     1021 tatacttaat aggtggaact tctgtattaa gtacaaatat agaaaaagaa ataaaagatt
     1081 taaaaatatc ttttgagaga atctctggag ctgagagata tcaaacatcc ttagaattag
     1141 caaagaaatt agatgctata agtgatgtta agaaaatagc tgtagttaat ggtgaaaaag
     1201 gacttgcaga tgctgtaagt gtaggagcac cagctgcaca aaacaatatg ccagtaatac
     1261 ttgctgattc taaaaatgga acagcagttg cagacaaatt tataaaggat gctggaataa
     1321 ctcaatctta tgtagtaggt ggagagagtt caatttctga agctgttaaa aataaactac
     1381 caaatagtac aagactaggt ggaacagata gaaatgatac aaatgcaaaa gtaataaaag
     1441 aattttataa aaagacagat ttaaaaaatg cttatgtaac taaagatggt atgaataaac
     1501 aagaccaact tatagatgca ctagcagtag gtgtattagg tgctaaaaat caatcaccag
     1561 tagttttagt tgggaagaat ttatcagctt cacaaaaatc tctagttaac tctaagagtt
     1621 ttgataagat aactaaagtt ggtgggaatg gtaatgagac agcatttaat gaaatgaagt
     1681 cattacaaga agtaaaaaca gttgaagcta aaacaatttc tgaattaaaa tcagctatag
     1741 ataaagctac tgctaatgat gttataaact ttaagccaac tagtgaggtt aaagaagcat
     1801 ttacaataca aactgataaa gcagtaacag taaacttaaa tggtacatat acaaagactg
     1861 taactatcaa catgcctaat ggagatgtaa ataactatgc aaaagtggat gatgtagtta
     1921 tagatgatgt aaaagatgga acttttgtta actatggaaa aataactaac ttaaaggtta
     1981 atgataaaaa tggagccaag atagaaaata attctaaagg tgagattggt agtttaacag
     2041 tagcatctgg agcaagtcaa gtaaaagtga ctaatggtgg taagataact acagttacaa
     2101 ataattctaa agggactact atagataata aaggtacaat aagtagtgtt aagggagata
     2161 attctccaac aataagtgga aatagtccaa gttctaactc ttcaggagga agttcatcaa
     2221 gtggaggctc ttcacatgga ggaggaagtt cttcatcaaa agtagataaa gtagttttga
     2281 aaaatacaat aacagcagca aataaattgt ataatgaagc aatagaggga acaaatgtag
     2341 gtgaatataa agtagggtca aaagcaatat ataaaacagc aatagacaaa gcacaggcaa
     2401 tattagacaa atctggtgta acacaaaaag aagtaaatga tgcagtaaca gcattaaaca
     2461 cagcaacaga cacatttaaa gcaggaaaag tagtagcagt agataaaaca gcattacaag
     2521 atgcagtaac agcagcaaca gcattgcatg ctaaagcaac agaaggaaca gcagagggag
     2581 aatatgctgt aggatcaaaa gcaacgtata aaacagcaat agatgaagca caggcaatat
     2641 tagacaaatc tgatgcaaca cagaaagaag taaatgatgc attatcagca ttaaacacag
     2701 caacagagac atttgaagca ggaaaagtag tagcagtaga taaaacagca ttacaagatg
     2761 caataacagc agcaacagca ttacataatg gagcaacaga aggaacatca gaaggaaatt
     2821 atgctgtagg atcaaaagca acgtataaaa cagcaataga tgaagcacag gcaatattag
     2881 acaaatctga tgcaacacag aaagaagtaa atgatgcatt atcagcatta aacacagcaa
     2941 cagagacatt tgaagcagga aaagtagtag cagtagataa aacagcatta caagatgcag
     3001 taacagcagc aacagcatta catgctaaag caacagaagg aacagcagaa ggagaatatg
     3061 ctgtaggatc aaaagcaaca tataaaacag caatagatga agcacaggca atattagaca
     3121 aatctgatgc aacacagaaa gaagtaaatg atgcattatc agcattaaac acagcaacag
     3181 agacatttga agcaggaaaa gtagtagcag tagataaaac agcattacaa gatgcagtaa
     3241 caacagcaac agcattacat aatggagcaa caaaaggaac atcagaagga aattatgctg
     3301 taggatcaaa agcaacatat aaaacagcaa tagatgaagc acaggcgata ttagacaaaa
     3361 caggtgcaac acaaaaagaa atagatgatg cattatcagc attaaacaca gcaacagaca
     3421 catttaaagc aggaaaagta gtattaaata aaacagcatt acaggatgca gtaacagaag
     3481 caacatcatt acatgctgga gcaacagaag gaacagcaga aggaaactat gctgtaggat
     3541 caaaagcaac atataaaaca gcaatagatg atgcacaagc aatattagat aaaacaggtg
     3601 caacacaaaa agaaatagat gatgcattat cagcattaaa cacagcaaca gacacattta
     3661 aagcaggaaa agtagtatta aataaaacag cattacaaga tgcagtaaca gaagcaacat
     3721 cattacatgc tggagcaaca gaaggaatag cagcaggaaa ctatgctgta ggatcaaaag
     3781 cgacatataa aacagcaata gatgaagcac aagcaatatt agataaagca gatgcaacac
     3841 aaaaagaaat agatgatgca gtaacagcat taaacacagc aacagccaca tttgaagcag
     3901 ggaaagtacc tactacaatt gcattaatgt tatcaagaat actaggattt atgaaataag
     3961 aaataaaaag caaattattt aataaaattt atatatactt taaatagaaa atttaattta
     4021 aggctgtgag taaaattttt aaaaataaat ttatacacag cctttttatc atgtaaagtt
     4081 aattgatata gtaagtttag ataatttaga gatacaaaat ttaaaatgtt agataaagtt
     4141 caaaatataa cctaatatca aaacttcaca taattttcat attaattatc aattaaattc
//
LOCUS       DAEMHZ010000219         4176 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000219 DAEMHZ010000000
VERSION     DAEMHZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.221"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            139..504
                     /locus_tag="KPK72_003679"
     CDS             139..504
                     /locus_tag="KPK72_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490845.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSSKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            582..1136
                     /locus_tag="KPK72_003680"
     CDS             582..1136
                     /locus_tag="KPK72_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2490846.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            1356..2030
                     /locus_tag="KPK72_003681"
     CDS             1356..2030
                     /locus_tag="KPK72_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2490847.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      2385..2481
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2535..2711
                     /locus_tag="KPK72_003682"
     CDS             2535..2711
                     /locus_tag="KPK72_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490848.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(2898..3137)
                     /locus_tag="KPK72_003683"
     CDS             complement(2898..3137)
                     /locus_tag="KPK72_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490849.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(3282..4091)
                     /locus_tag="KPK72_003684"
     CDS             complement(3282..4091)
                     /locus_tag="KPK72_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2490850.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         1535 a          577 c          499 g         1565 t
ORIGIN      
        1 ttatgtacta aatatgaact acaatagaag aaatttagaa cttaataaaa cataaaattt
       61 atatacagtt catatatatt tgtcatatca caagacaagt gatataattt attatcattt
      121 tactgggggt gatatgctat gaaaataaga gctgaatata catgtcctct tgaaattgta
      181 catgacataa tcaaaggaaa atggaaaact atccttatat ttgaattaag acaatctagt
      241 aaaacatttt ctgaattgca acataatatt tatggaataa atcaaaaaat gttgattcaa
      301 caattaaaag agttacgtga ttttgggatt attgataaaa taagttctgc tggatatcca
      361 ttacatgttg aatattttct tactaatcgt ggtaaaaaaa tgttaaaagc agtggaaata
      421 atgcaagaaa taggaatcga atatatgctt gaacatggtc aacaagaaat tctagataat
      481 aaaggaattt gctacaataa gtaatcctat acataccaaa aagtaagtaa tttacatttt
      541 ttacaatagt attataataa aaataaatag gaggtataat catgtctata gctttaatat
      601 tgattgatat tcaaaatgat tattttaaaa atggaaaatg tgaattattt caatcagaag
      661 aaactgctga aaatgctaaa aaaatactac tgttttttag aaaaaacaat ttaccagtta
      721 ttcatataca gcatatatca acagataaaa cagcttcttt ttttcttcct aatactcatg
      781 gttcagaaat aaataaaatt gtttttccac tagataatga agaaattata attaaacaca
      841 caccagatag tttctttgaa acagatttgc aaagtatcct tgagaaaaaa aatattacta
      901 atctagtaat ctgtggaatg atgagccata tgtgtattga tacaagtgta agaacagcaa
      961 agaaacttcg atacgacata actctaataa gcgatgcatg tacaacgaag aatttatctt
     1021 ggaataatat agaaattaat gcaaatactg tacatcaagt attcatggca tcactacaga
     1081 acagttttgc agatgtaaaa aacacagata aatttttatc taactattac aaataaaaac
     1141 atacactatg ttaaaagagc aagtttgatt tatttaagct tgctctttaa tcatttaaaa
     1201 ttagatgctt tctatcattt ttatatacat aatatacgtt tatacatttt atattcatat
     1261 ataactatta tataaatttt aaactttact atattcacat aaaaaatgta gtattatagt
     1321 atatataata ttatatagaa gggggtgcta gataaatgaa ccatcatata ttgattcaat
     1381 ttgaatatat agcaagaatc attactgctg gtttctgtgg ggctttaatt ggatatgaga
     1441 gaaagaatag actaaaagaa gctggaatac gcactcattt tatagtggca ttagcttcag
     1501 cacttataat cattgtttcc aagtatggat ttaatgatat agtgggtact ccaggtattg
     1561 gtctagaccc agcccgcgtt gcagcacaag ttgtcagtgg tataggtttt ttaggtgctg
     1621 gattaatatt tattagaaat caatcagtaa gtggtcttac tacagctgct ggtatatggg
     1681 caacagccgg tacaggtctt gccattggct ctggattata cttagttggt attgtttcaa
     1741 gtatcattat tgttattgta cagataacac ttcatagaga taggaaatgg atgaaactcc
     1801 ctacagcaga gcaagtttct cttaaaattt ctaacagcac tgattccata gaatatattg
     1861 aaggtaaatt taaaaaatct aatattgaaa ttataaacat gaatattaaa cgccttgatg
     1921 gggattggtt agaagtagtt atatatacta agttacctaa aggatataaa aaaacaaatt
     1981 tattaaaatt atttagcgat aatcctctta tagaaacttt agaaatataa tataaaaatt
     2041 gaagcttaaa aataataata tatttaatca aggtcaatat ataaataatt acgggatgag
     2101 tctatataaa attcatccct tgttttttgc cattcatata tattttttca agatattttt
     2161 gttcatctat tatctagcca agtaactctt gtgaactaaa ctttttaata caaaaacttc
     2221 aatacaaaac taaatataac ttttattaaa cattctacat atatagtttg tttcttgagt
     2281 gaaaaataga tttcatattg ttaagatact gttaaatatt tcaattttct gacaagtatg
     2341 ttgaacaata acttatccta gtgtattatt ttagttgtag aataaaacta aatcggcaaa
     2401 actagagaaa tttagtgacg caaagctata gggactaacg cttataaaat aaactatgag
     2461 ttatgtcagc cagttgccaa aaagatattg ttctttttgt tttatgaaag ttttttaggg
     2521 ggatagtaat atatatgttt agagatgaaa tggataaatg tacacacatg ttaactgctt
     2581 atattagtag tttatatgat tattgtgatt ttatagatac acagctagat gattttatac
     2641 tagagtacgg agaaaatgta gtagaatctt gtttacatca agtgatggta ttggtaagta
     2701 agtataatta aataaaaatt gttttctaga gaaccctttt tagttttatc tacatttaaa
     2761 aatgttatta tattaaaaaa ctaatctccc ctatatgcaa agtttcttcc atataacaat
     2821 ttcttcccat ttatactttt tcacttgtat ctttaataaa attcaacttt aaatattaat
     2881 atacttcaaa aattattcta tttcttatct ttacaccaat tcctatcagt attatccttt
     2941 atataggttc taactatttc gccacaatct aaacataaat ctgaatatat agtagattgt
     3001 actggaaaag ttttatttat aactttatct aattttccat ctcctctata ttttaatcca
     3061 atagtttcag catatatgcc accagttgtt aaaattccct tttcaatatt tacacttcca
     3121 caattagaac atttcacatt tataaccccc taaacaatta ttttttcatg ctatcttata
     3181 atttaaaata taatttgcta ccgattttat aacacagtgg caaatcggtg gcaaatcaac
     3241 attttttcat tctatttacc atctaatcca ttgaaatata gctatttagt tataggaact
     3301 tgaatttctg ttacaaactc ctctacaatt ccagtctcat gtatatctat cttatagatt
     3361 tctatagggt cagcaattat cttataatta ttttcttcta taaatttaaa caccttctcc
     3421 atatatatct tattattaga attactccca gtataattta aagttacata atatccttca
     3481 tcaaaaacta tattatatac tttctcatta ctttccagaa aacaaaatac agatttaaac
     3541 tcattaaata tacctttatt aattgcttca gtagaaaagg cagaaccaat attgttattt
     3601 ccaagtatat taaatctatc ttcatattct ttctgaagtt tttgaattaa aaaatcgaag
     3661 tcttcatccc tctttatatc agcatttaat tctaaagctt ttcttttttc tatatataca
     3721 acctctatct catctatttt tgtattttga attacacttt caattgaact caatcttttt
     3781 ataatatttt ccttatggct tataaattca gctatttttt tatcaattaa atcgagttct
     3841 tcatttaaaa tatttttcgt agactctata cttctgtcat ctaggtattc ttttattttc
     3901 ttcattggaa agttcaagct tctaaattct tttattaaat ttaatttcca tatatctgat
     3961 atattataca ttctatatcc atttatatct ctaactggtc ttagaatgcc tatttcttca
     4021 taatacatta atgaatctct acctattcca tatattttag atatctctcc tattttatag
     4081 taatctttca tttatataat aaactcctcc gaaatcctat ttttatttta aatcaacatt
     4141 attactctat aatactatag tattagaata tactaa
//
LOCUS       DAEMHZ010000220         4093 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000220 DAEMHZ010000000
VERSION     DAEMHZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.222"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            16..414
                     /locus_tag="KPK72_003685"
     CDS             16..414
                     /locus_tag="KPK72_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2490851.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            402..1340
                     /locus_tag="KPK72_003686"
     CDS             402..1340
                     /locus_tag="KPK72_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2490852.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1724..1948)
                     /gene="secG"
                     /locus_tag="KPK72_003687"
     CDS             complement(1724..1948)
                     /gene="secG"
                     /locus_tag="KPK72_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG2490853.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2553..3845)
                     /gene="eno"
                     /locus_tag="KPK72_003688"
     CDS             complement(2553..3845)
                     /gene="eno"
                     /locus_tag="KPK72_003688"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG2490854.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1413 a          654 c          459 g         1567 t
ORIGIN      
        1 tcttaaggag ttgccatgat aaaaaaaata gaacatgtgg tcattactaa taataatata
       61 gaaaattata tcaattttta taaaaaattt ggtttttcat ctaaagaaat gagcaaaaat
      121 tataaactta atgctagaaa tgttaagata aatcttaaca taaaaggtta taaattttta
      181 ccttatgtta aaaatttaca agcaagtaga gataacttat gttttaaaat aaacacaaat
      241 attgatgagc caaaaaaata cttagaacaa aatgagattc aaattaagag aggaattgta
      301 gaatgtacag gtgtctttgg taatatgaaa tctatttatt tgaaaaaaac taatggaaat
      361 ttaattgaat tatcaaatca tagaatcacg gagagtaaac cttggaaaat ttaatcctat
      421 caataaatgt tgttcttcct ttgtttctca caatgtcact tggatatgtt ctaaaaaaat
      481 taggtctatt tgatactcat actttaaata aaatgaataa tgtctgcttc aagtctttct
      541 tgccattatt attatttttt aatatataca atacagattt agatggttct cttaatttta
      601 agcttatggc tgtagctgtt ataattattt tttcaatctt cttaatctta gtctttatta
      661 ttcctaaaat tgaaaaagat aataagacaa ggggtgcatt aatacaaggt gtatttcgta
      721 gcaatttcgt tatatttggt attcctgtga caatttcact atttggcgat aagagtattg
      781 gagtcacatc tttattaatt gcagttattg tacctatgtt taatgtatta tctgttgtaa
      841 ttcttgaaat ttttagaggt ggcaaagtaa atataaaaca tatgattaaa agcgtcttaa
      901 caaatccact cataatcgcc tcttttatag gattgacaat gctaatttta aatattaata
      961 tacctacatt tttacacaaa actattggtg atatttctaa aatagctact cctttatcac
     1021 ttataattct aggcggctct tttagttttt ctgctataaa ggggcatata aaacccatta
     1081 tattgggcgt atgtggaaaa cttatacttg tacctgctat atttttacca attagcattt
     1141 ttcttggatt tagaaatgta gaattagctt cacttatgat aatgttatct gctccaactg
     1201 cagtttcttc atttacaatg gcacaacaga tggattctga cagtgaatta gctggacaaa
     1261 ttgtagtatt tacttctgga ttttctataa ttacagtatt tttaataata tttattttaa
     1321 agcagaataa tcttatttaa tgatatttga taacacaaat tggtattatg agaaacatat
     1381 ataaaattga tttaattgcg taaaaacata ttattatact atttttacta tataatataa
     1441 ctttgtgtat attatctcat taaacatggt gaaagtagtg ttgctatata ttttttagtt
     1501 tgaagaaaat atataaatac aaacaatata taaatacaaa taataaacgc caacaaatag
     1561 tcaatctata aaataaaaac tggactaatc atataaaatc attagtccag cttttatttt
     1621 acgtactttt tatgttgatg tacttttatt aaaaactaaa tttaactttt aaatgtctag
     1681 caaatgtact aattaaataa tggccagtta ttcaacaata tcactacttc ttaactataa
     1741 gtaatgccaa tgcatttata aaaaataata ctgctgttat ctttgtaact ctagcaagaa
     1801 atgcttgttt tccttttggt ttaaagtaag cttgacttcc ttctccacca aattctgaag
     1861 gtaaagcatt cttagaatct tgaggcatta tgctaattac tagtaaaatc cctagtacta
     1921 cttgcacacc cattaaaatg ttgctcattc ttgcccctcc tatctgttta ttacttaaat
     1981 ttcattactc tttagtgtat cacaataata tagccatttc aagactatta tgctataaat
     2041 aacatacaaa aaaatatatt gttactataa tatattacta tattctcaat ataaaaatga
     2101 ataaaactta gaagagaaaa ctatttaaat taatacataa attaatgcat aaattaacac
     2161 ttaaaataat ctactctcat tgcatatatt ataaatagcc accaaaataa aacaatcatt
     2221 ttaaaatatt gatataactt ttaatttcaa cataatttaa aataaataat ccacatttag
     2281 caataatact ttaatgacaa ttatgattaa atcaaaatat gtcttcactt ttatttttta
     2341 ataaaaacct cctatgcaat tagataaaaa tgagatagaa acatttattc atattttttc
     2401 tgaatctatg aataaaatta agtaataaaa tataaaaaag gataagtgaa ttgaaaaatc
     2461 aattaacact tatctctttt atatcttata agatgagctt aaatatctta tattctctct
     2521 tcattaatct tttttagtaa ccattttaat aactattttt ttaagttata gaaagatttt
     2581 aatccacaat atctagcttg ctctccaacc atttcttcta ttcttaataa ttggttgtac
     2641 ttagcaactc tatcagttct tgatggagca ccagttttta tttgaccagc atttactgca
     2701 acagctaaat ctgctatagt agaatcttca gtttctcctg atctgtgaga tataacagca
     2761 gtatatcctg ctctcttagc catttctata gcatctaaag tttcagttat tgtacctatt
     2821 tgattaactt taactaatat agagttagct actccatttt caattccttt ttctaatctc
     2881 tctgtgttag taacaaataa gtcgtcacca actaattgta atttattacc taatttttca
     2941 gttaataatt tccatccatc ccaatcttct tcgtctaaac catcttctat agttattatt
     3001 gggaagttgt ttgaccaatc ttcatataaa gcaaccattt cagcagcagt taactctttt
     3061 ccttctcctg ctaaaacgta ttttttagtt tctttgttgt acatttctgt agcagcaacg
     3121 tcaagaccta acataacgtc ttcacctggc ttatatccag cttttgttat agcttcaact
     3181 attaattcta aagcttctct gtttgaacct aagtttggag caaatccacc ttcgtcacct
     3241 acaccacaag ctaatccttt ttctcctaaa actttcttta atgaatggaa tacttctgct
     3301 cccattctta aaccttcttt gaaacaacaa gctcctactg gtaatatcat aaattcttga
     3361 acatcaacgt tgttatcagc atgctctcca ccgtttaata tgttcatcat tggaacaggt
     3421 aattgtttag catttactcc acctaagtat tggaataatg gtaaacctat ttcatcagca
     3481 gctgctctag ctactgccat tgaaacacct aatatagcat tagctcctaa tttacctttg
     3541 tttggagttc catctaattc tatcatgatt gcatctatag ctggttggtc aaatgcatcc
     3601 attccttcta tttcaggtgc tattatttca tttacgttag caacagctgt ttctacacct
     3661 tttcctaagt atcttccctt atctccatct cttaattcaa cagcttcaaa agctcctgta
     3721 gatgctcctg atggaactat tgctcttccc attgctccat cttctaatac tacttcaact
     3781 tctacagttg gatttcctct tgagtctaat acttctctag catatactaa ttcaataact
     3841 gacatttgta tatctccctt cgattttcta ttagttaaat tttcatttac tgtaaacatc
     3901 cgtctactat tttaatctac tttctatatt atagtacata aagttgcttt tgaaaattag
     3961 gtacaaattt atttgatggg taaatttgta aaagttccta tttttatttt attttttata
     4021 atattttaat ttatataata taaaaaaact tgtctaaaat ccaaaaccaa tcttaaatac
     4081 acaaatatta taa
//
LOCUS       DAEMHZ010000221         4074 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000221 DAEMHZ010000000
VERSION     DAEMHZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.223"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            228..1151
                     /locus_tag="KPK72_003689"
     CDS             228..1151
                     /locus_tag="KPK72_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG2490855.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKVG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1126..1953
                     /gene="panB"
                     /locus_tag="KPK72_003690"
     CDS             1126..1953
                     /gene="panB"
                     /locus_tag="KPK72_003690"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG2490856.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1972..2820
                     /locus_tag="KPK72_003691"
     CDS             1972..2820
                     /locus_tag="KPK72_003691"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG2490857.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3231..3413
                     /locus_tag="KPK72_003692"
     CDS             3231..3413
                     /locus_tag="KPK72_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490858.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT         1596 a          485 c          577 g         1416 t
ORIGIN      
        1 taaatatcat aattttttta ctgatatttt attatataaa ttttatagaa ttaattgtca
       61 attttttatg aaaatggtaa gatatttatg gatggtatct tgttataaga actatacttt
      121 aaatgttttt aattattttg tcttatgtaa ctcatatcct caggaatgag attagaaatt
      181 aagatgttgt atcacaaagt ggaggtgata ctattaaaat aggctttatt ggttctggaa
      241 aagttggaac atctttggca aattattttt tatcaaaagg ttattgtgtt tcaggctttt
      301 atggaaggaa tcaattatct ttattagaat caacaaattt aacaaaaaca aagatttaca
      361 gcaatttgaa agatataatt tatgaaaacg atatattatt tattacaaca tcagatgatt
      421 ctattgaaat tatagacaaa aaactttcca aatttaattt aaaacataaa tacatttgcc
      481 acacaagtgg ctctttaaaa tctagtatat tgttctgttc aaaaaaagta ggtgcattaa
      541 tttattctat ccatcctata tttgcatttt ccaataaaaa taccaacata aaaaaaatga
      601 aagacatttg tttttccata gaaggtgata atgaacaaga tgatttagtt attcaaaatt
      661 tcatagatga tttgggaaac caatttttta taagagataa ggatacttca tccacttatc
      721 atttagctaa tgttttagtt tcaaatttag tattatcttt actagatata ggagtaagtt
      781 actttattaa attaggttta tctgaagagg aatctttaaa agctatcagt cctttagtaa
      841 aaaaaaatat agaaaatata cttgataatg gctttactaa agctcttact ggtcctgttt
      901 ctcgtggaga tataacacca ataagaaaac atctatctgt attggataaa aaagatgagg
      961 atatttacaa aatactatcc ttaaacctac taaaaataat tgccttagga aatgacaatt
     1021 cattaaaagg tataaaaaat attactatag aaaatgcaat cgaaaattta ataagtaaat
     1081 ctgaaaaata caaagaaatt tacaaacttt taggaggaaa aaacgatgaa aaataccata
     1141 cagactttta agaatgctaa atatgaagga aaaaaactat ctatgcttac tgcttatgac
     1201 tactcaattg caaaaataat ggatgagtgt gatataaatg gtattttaat aggagattca
     1261 ttaggaatgg ttataaaagg tgaagaaaac actctatctg ttaccataga cgaaattata
     1321 tatcatacaa aagctgtaaa aaatggtgta aaaaatgcct taatcgtaag tgatatgcct
     1381 tttctatctt accatgtttc tatagaagat gcagttaaaa acgctggtag acttataaaa
     1441 gaaggaggcg ctcatgctgt aaagttagaa ggtggctcaa acgttataaa gcaaatagaa
     1501 agcattgtaa atgctcaaat acctgtaatg gggcatttag gattaactcc tcaatcagta
     1561 aattcatttg gtgggtttaa ggttcaagga aataccagtg aaacagcaag acaacttatt
     1621 gaagatgcta aattaataga aaaagcaggt gcattctcta ttgtccttga aggagtgcct
     1681 acgaaaatag ctgagatggt aactaattct atttcaattc ctacaatagg tataggggca
     1741 ggaattaact gtgatggaca aattttagtt tatcaagata tgttgggaat gtttggagat
     1801 tttgttccta agtttgtaaa gcagtatgca aatataggag atataatgaa agattcaatt
     1861 aaaaattaca tcctagaagt taatacaggt acatttccac aagaaaaaca cagcttttca
     1921 ataaatgaat ctgagttaga aaaattatac taatattaag gatggtacaa aatgcttgta
     1981 aaagaaatta aattactaag aaatataatc aaagattgga gaaaacatgg ctattctata
     2041 gggcttgtta caacaatggg atttttacat gaaggacacc aaagtcttat aaaaaaagct
     2101 gtaaaagaaa atgataaagt agtagtgagt gtatttgtaa atccaactca atttggacca
     2161 aatgaagatt ttaatagcta tccaagagat atagataaag attttaaata ttgtatggat
     2221 agtggagcaa cagtagtctt taacccatca ccagaagaaa tgtatttaaa aggtaattgt
     2281 actaccatta atgtttctgg acttacagat tttctttgtg gagctaaaag acctgttcat
     2341 tttggaggtg tctgcctagt tgtttcaaag tttctaaata tagtaacacc tgataaagct
     2401 tattttggag agaaggatgc ccaacaatta gctgtaatta aaagaatggt caaggattta
     2461 aacatagata cagaaataat aggctgtcct ataattcgtg aaaatgatgg attagcaaaa
     2521 agttcgagaa atacatatct ctctgaggaa gaacgaaaat ctgctctaat attaaataag
     2581 agcttaagtt tagcaaaaga agaattagta aaaggcaatc taaatccaga gaatataaaa
     2641 gaattaataa cagctaaaat aaattctgaa cacttagcaa aaatagacta tgttgaaatt
     2701 gtagatagtg agacattaca acctgttaag caaatagaac attctatact tgtggcaatt
     2761 gctgtgttta taggaaaaac cagactaatt gataatttta catttgagtt aaatatatag
     2821 tctataattt tatataatat atctaaaaaa taatagaata cctaaaaagt aataaattac
     2881 ctaaaaaata ataaattgct tagaaaataa taaattacaa taactaaaat tttaaaatta
     2941 aattccttaa aagtatgatt aattataatt atataactat attcttatta cacttatatt
     3001 aatttatatt aaatatctac taactcaaat attcataatt aaactttaaa ctggataaat
     3061 tttcttttaa gaatgaaagt ttatccagtt ttgcgattat aataaccagt tttacatatc
     3121 cacttgtaaa atatatctaa gcaatatatc atactatata gatttatgag tgaaataata
     3181 tctgctattt actgaatatt tttatttttc gttttttagg gggattagtt gtgtttaaag
     3241 ataagattga tgaatgtgtt catataatga ctgcatatat tgctagttta aaagaatact
     3301 attcattcat agaaactcaa attggtgatt ttattaaaaa atatggagaa gatgttgtag
     3361 agttatgtct ccatagagtg atgattctac tatgcgaatg tggtttggca taaaattaaa
     3421 tttattaaaa tgggtaagaa gaatcctata taatattctt cttacccatt ttaatatatc
     3481 aatttcatag cttatcgttt aaaatatttt acatccatta ctagacaact ttacattctc
     3541 tttttaattc tagtatattt aaatcatttt agttgctcct tatttattct tattacttgt
     3601 tttattttat aaataaaata attcttttta ataatgtttt ttgttgatat tcctatcaca
     3661 catccaatta tttatcattg tcttgaaatt gtttgcatac ttaatttaat ccaattgaaa
     3721 caccccagca tagaatcccc tgtaaaatag aagttggatt aaatcctaac atctatacag
     3781 aaaatactac tccaagcaca agcaatataa tggaatatat ttatcactta attttttata
     3841 ctttttaaat cccataccta aaacattaag tgcagctatt agaattaata attgttgtgg
     3901 tataaaatta tgcttgcttc atatttattc attttttact ataaaagttc ttgttttaca
     3961 aaaaacattt gttttgtatc aaaataatac ttataaatta cataattctc ataaaaattt
     4021 ctagtattac ttgtactctt aataaataat agccaacaag taccttaaat catt
//
LOCUS       DAEMHZ010000222         4058 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000222 DAEMHZ010000000
VERSION     DAEMHZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.224"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            4..297
                     /locus_tag="KPK72_003693"
     CDS             4..297
                     /locus_tag="KPK72_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490859.1"
                     /translation="MNRANRIIYNQDGKILLQTGEATGDILEHDTITELHCIDVPYGN
                     IDYTKNRIIGINIETKEPILEEIPIFVSEEEKRIQELENQLLLNENEKVGGLL"
     gene            297..479
                     /locus_tag="KPK72_003694"
     CDS             297..479
                     /locus_tag="KPK72_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490860.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIKQSGVVEGIEPTV"
     gene            517..747
                     /locus_tag="KPK72_003695"
     CDS             517..747
                     /locus_tag="KPK72_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG2490861.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            767..1027
                     /locus_tag="KPK72_003696"
     CDS             767..1027
                     /locus_tag="KPK72_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2490862.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVILLIL
                     GVGFSVWMLGLNPSAVLQGIICWGISIGINQTYKQLKEENKQ"
     gene            1024..1821
                     /locus_tag="KPK72_003697"
     CDS             1024..1821
                     /locus_tag="KPK72_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2490863.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKIDKSNNYLAEQCEIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNDKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNSEDKKMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYI
                     PGQTENLYVVGGGACEKISFITKEHYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(1843..2004)
                     /locus_tag="KPK72_003698"
     CDS             complement(1843..2004)
                     /locus_tag="KPK72_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2490864.1"
                     /translation="MKKILYRFMKACGVLAMFVAFLSANTTSLWHVYQPKTPEKLKNS
                     KNHQKEGNN"
     gene            complement(2017..2604)
                     /locus_tag="KPK72_003699"
     CDS             complement(2017..2604)
                     /locus_tag="KPK72_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2490865.1"
                     /translation="MIKSCADKIAFLFVLNQVIEKDDLEICSYGLEILLSSIINTLSS
                     LMIGLILGRLIYSIVFLISFCIIRQFSGGYHANSNKNCILIFNSIFFISMVLGIILNN
                     SNPIPLFLVTLSSFNFLIIFIFAPVCHTNNPLNKTKYIKNKLMSRIISITLFLIILLG
                     CKYLPFYNYVVYTFLALLWINLLLIIQIIINKKII"
     gene            complement(2647..3984)
                     /locus_tag="KPK72_003700"
     CDS             complement(2647..3984)
                     /locus_tag="KPK72_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2490866.1"
                     /translation="MESFIMLDFFITIVSSIIEIWACKKVFDYTSKVKTSLINLYFLI
                     STFIIISTFYTNVGADNRILICIFVMFIFYKLNYDVNIYKCLIVLFSYWAILVCINAL
                     SMIVIVSINNLSSMNILLNDNFYRYETIVLGNVVLIILLCIYKFISNDLQVTKREIYM
                     CIPIVANMISFFIFYKYILKLLDISSTTRLDILIMVLLLTFSNIALISSLVKILKSNK
                     KYIELTKLQEKINAENKYYSNIKKYYIKTKELNHDMKNHLICIKEMNKKNIDTSNYIN
                     NIEKKLDEYSAVFDTGNITLNIILSEKKELCIEKGIDFTININFSDCNFIKEEDTCSI
                     FANALDNAIEACEKIKNDDKKIKLQGMIVNKFFVIKITNTKSNDILLKKNTFLTTKED
                     KEFHGLGIKSIKNSLEKYGGAISINFSENLFCLNMMIPIRKSQKDSNIAYENS"
BASE COUNT         1636 a          452 c          573 g         1397 t
ORIGIN      
        1 aaaatgaata gagcaaatag aataatatat aatcaagatg gaaaaatatt actccaaaca
       61 ggagaagcaa caggagatat attagagcat gatacaataa cagaattaca ttgtattgat
      121 gttccatatg gaaatataga ttatactaaa aatagaatta taggtataaa tatagaaaca
      181 aaagaaccaa ttttggaaga aataccaata ttcgtttcag aagaagaaaa gagaatacaa
      241 gagttagaaa accaattatt actaaatgaa aatgaaaaag taggaggatt attataatga
      301 atataaataa tgttgtagta agaatattag cagagaggat tttaaacgga ggcttaaacc
      361 ctctaaaaaa tcgagaattt gaattagatg atgtaactaa cacagagtat agaaaagcag
      421 tagaggatta tataattaaa cagagtggag tagtagaagg aatagaacct acagtatagg
      481 taatgttctt tttttaatac aaataaggag gtttacatga atgaagaact tttcgaagca
      541 gatttaaaaa gacatgaaac aagaataaat aaacatggag aagaaataga cgaattaaaa
      601 atagcaaata tagagtctaa agcagagtta aaagcattgt gtgagaatct aaattcactt
      661 acaagtatgc tcaaatggct aattggaaca atgattacaa cacttgtagg attctttata
      721 tttgcagttc aaagaggaat attttaatta attaggagga taaaagatgg ataatttaat
      781 aagttttata ccagagcagt tgctaatttt agtggctgct ctttatgtta taggagcagg
      841 ttgcaagaaa tataagcaat tagataacaa gtatattcca gttatattat taatacttgg
      901 tgtaggtttc tcggtgtgga tgttaggatt aaatcctagt gcagtcttac agggaataat
      961 ttgttgggga atatcaatag gtataaatca aacttacaaa cagttgaagg aggaaaataa
     1021 acaatgaaaa tagcaatagt accaggacac actttaagtg gaaaaggaac aggagcaact
     1081 gggtatatag acgaaggaaa agaaaacaga atactaactg atttaatagt aaaatggttg
     1141 aaacaaggtg gagctattgt gtatactgga aaaatagata aatctaataa ctatttagcg
     1201 gaacaatgtg aaattgccaa taggcaaaat gtagacttag cagtacaaat ccatttcaac
     1261 gcaaataaaa caactctaaa tcctatgggt acagagacaa tttataaaac taataatggg
     1321 aaagtctatg ctgaaagagt aaatgataaa ttagcaacag tatttaaaaa tagaggtgca
     1381 aaatcagatg tcagaggtct ttactggctt agtcatacaa aagctccagc gatattaata
     1441 gaagtgtgct tcgtagatag taaagcagat acagactatt atattaggca taaagacata
     1501 gtcgctaaat taatagcaga aggtatttta aataagacaa tagataatag tgaggataag
     1561 aaaatgtata aacatacaat cgtttatgat ggagaagttg acaaaatccc tgcaactgta
     1621 gttggttggg gctataatga tggaaaaata ctgatatgtg atataaaaga ttatatacct
     1681 ggccagacag agaatttata tgttgtcggc ggtggagcat gtgaaaagat aagttttata
     1741 acaaaagaac attatacaat gataaaaggt aatgataggt ttgatacact ttataaagca
     1801 ttagatttta ttgatagata gattagaagg tagcaactag agttagttgt taccttcttt
     1861 ttggtgattt ttagaatttt ttagtttttc tggtgttttt ggttgataca catgccacag
     1921 agaagttgtg tttgcagata aaaatgctac aaacatagct aatacaccac acgctttcat
     1981 aaaacgatat agaatctttt tcatgttagt atctccttag attatttttt tatttataat
     2041 gatttgaata attagtaata aatttatcca aagtaaagct aaaaaagtgt atacaacata
     2101 attgtaaaaa ggtaagtatt tgcatcctaa aagaataatc aagaataaag ttatagatat
     2161 aattcttgac attaacttat tttttatata ttttgtttta ttcagtggat tattagtgtg
     2221 acatactgga gcaaaaataa aaatgattag aaagttaaaa ctagacaatg tcactaaaaa
     2281 taatggaata ggattagaat tatttaaaat tattcccaaa accatagaga taaaaaaaat
     2341 actattgaaa attaggatac agtttttatt gctatttgca tgatatccac ctgaaaattg
     2401 tcttattata caaaaagata ttaagaatac tatagaataa atcaatctac ctaatattaa
     2461 acctatcatt aatgaagata gagtatttat tatggaagat agtaaaattt ctaacccata
     2521 tgaacagatt tctaaatcat ctttttctat tacttgattt aaaacaaaca aaaaagctat
     2581 tttatcagca caagatttta tcatttagtt ttctccttaa ataattttat taataaatta
     2641 tggatattaa ctattctcat atgcaatatt tgaatctttt tgtgattttc taattggtat
     2701 catcatattt aagcaaaata aattttcaga gaaatttata gaaattgcac ccccatattt
     2761 ttctaaagaa ttttttatac tttttattcc taatccatga aattctttat cttcttttgt
     2821 agttaaaaaa gtattctttt ttaataaaat atcatttgat tttgtattag taatttttat
     2881 tacaaagaat ttatttacta tcattccctg caatttgatt tttttatcat catttttgat
     2941 tttttcacaa gcttcaattg cattatctaa tgcatttgca aatatagaac atgtatcttc
     3001 ttccttaata aaattacaat cactaaagtt tatattaatt gtaaaatcaa ttcctttttc
     3061 tatacaaagt tctttttttt ctgataatat aatgtttaaa gtaatatttc ctgtatcaaa
     3121 tacagcacta tattcatcaa gctttttttc tatgttgttt atgtaattac tggtatcaat
     3181 attcttttta ttcatttcct taatgcaaat aagatgattt ttcatatcat ggtttaattc
     3241 ttttgtctta atataatatt tttttatatt actataatat ttattttcag catttatttt
     3301 ttcctgtaat ttagttaact caatatattt tttattactt tttaatattt taactaggct
     3361 agaaatcaat gctatattag aaaatgttaa taatagtacc ataattaaga tatctaatct
     3421 ggtagtggaa gaaatatcta gaagttttaa gatatattta taaaatataa aaaatgatat
     3481 catatttgct actattggta tgcacatata aatttctctt ttagtcactt gcaaatcatt
     3541 agatatgaac ttataaatgc ataacaaaat aattagaact acatttccta aaactatagt
     3601 ttcgtatcta taaaaattat catttaataa aatattcata gaactcaaat tatttatcga
     3661 aactattact atcatactta atgcatttat gcacaccagt attgcccagt aagaaaacaa
     3721 aactattaaa catttgtata tattaacatc ataatttaat ttataaaaaa taaacattac
     3781 aaaaatgcat atcaatattc tattatcagc accaacatta gtatagaaag tagaaataat
     3841 gataaaagtg gaaattaaaa aatataaatt tattaaacta gtttttactt tacttgtata
     3901 atcaaatact tttttgcatg cccaaatttc tataatagaa gaaacaattg ttataaaaaa
     3961 atctaacata ataaaactct ccataactta agtaattttg tataaatttc atttaataat
     4021 acgaaaacaa tttgaaattt tatagtaatt atatcata
//
LOCUS       DAEMHZ010000223         4003 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000223 DAEMHZ010000000
VERSION     DAEMHZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.225"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..370
                     /locus_tag="KPK72_003701"
     CDS             167..370
                     /locus_tag="KPK72_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490867.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            534..1688
                     /locus_tag="KPK72_003702"
     CDS             534..1688
                     /locus_tag="KPK72_003702"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG2490868.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1701..2705
                     /gene="rseP"
                     /locus_tag="KPK72_003703"
     CDS             1701..2705
                     /gene="rseP"
                     /locus_tag="KPK72_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG2490869.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2888..3943
                     /gene="ispG"
                     /locus_tag="KPK72_003704"
                     /gene_synonym="gcpE"
     CDS             2888..3943
                     /gene="ispG"
                     /locus_tag="KPK72_003704"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG2490870.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIKQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVEYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         1555 a          394 c          780 g         1274 t
ORIGIN      
        1 tatataaagt ttaatttttg aacttatgct tttgttttat tttaacatat ttatatttaa
       61 taaactataa ttgaaatcta tttagataaa taattattta tctaaataga tgaaaatttt
      121 ccaaaataga taaaatgtga tagaatatat tttgcgaggt gataacatga tttcatacgc
      181 tccattatgg aaattgttaa ttgacagaaa aataaagaaa atggaatttg taaatatatc
      241 aggtataagt atttctgttt taggcagact aggaaatgat aaatctgttt ctatggatac
      301 aatggagaag atatgtttag cactagactg taaaatagaa gatgttgtgg aaataaaaaa
      361 gggcatttaa taatactaga atattttata atattccaca aaatacaaaa aataatagat
      421 taattttaat ctgtggaata atatatgaaa tttctatgaa tagtaaaaat aattgtttta
      481 gtagtgttat aatgttttga cagggtatat ttattattag aaaggattat tatatgaaaa
      541 agatatcaat tttaggttct acaggctcta taggtaaaca aactttggat gtggttagag
      601 ataatagaga taagtttgag atagttgcaa tatcagctaa cagtaatatt gaattattgc
      661 tagaacagat agtagagttt aaacctaaat atgtaacagt ttttgaggaa aataaagcat
      721 tgaaattaaa agaaatgttg cctaaaaata tagaaataga agttttagca ggaatggaag
      781 gattaaaaat aatatcatca cttgatgaag tggatgtcct tttaactgct gtagtaggta
      841 tgataggatt agtcccaact ctttgtgcta taaagaaggg aatagacata gcattagcga
      901 ataaagaaac gttggtaact gctggtgaac ttgtaatgaa agaagcagaa aaatacaatg
      961 taaatattct tcctgttgat agtgaacata gtgctatatt tcaatgtcta aatggagaaa
     1021 ataaaaaaaa tatagaaaag ataatactta cagcatctgg tggtccattt agaggaaaga
     1081 aaaagggaga acttgcaaat ataactaaaa atgaagcttt gaagcatcca aattggagta
     1141 tgggaagaaa aataagtatt gattcttcaa cgcttatgaa taaaggactt gaagtgatag
     1201 aagctagatg gttatttgga gtagaacaag aaaatataga tgtagtagtt cacccacaaa
     1261 gcataattca ttcaatggtg cagtatacag atagctcaat aattgctcaa ttaggatgtc
     1321 cagatatgag actaccaata cagtatgctc ttacatatcc agatagaatg gaaagtagct
     1381 ttgaaagaat gaatttttca aaatttagta ctttaacttt tgaagaacca gatttagaaa
     1441 cttttccatg tttaaaattg gcatatgagt gtttaaaaat gggaggaact tattcttctg
     1501 ttttaaattc agcaaatgaa gttttagtaa gtgaattttt agaggataaa attggtttct
     1561 atgacatacc atattacata gagaagactt tagaagttca tagtagcata agtgaaccaa
     1621 cattagaaga aatcttagaa acagatagat ggagtagagc ttatgttgca aatctgataa
     1681 aaaaatagga aggtgaattt atgactataa tagccgcatt aatactattt agtataattg
     1741 tattaataca tgagttagga cattttatat ttgcaaaaag aagtggtatc aaagtaaatg
     1801 agttttctat aggtatgggg cctaagatat atagtgtaaa aaaagataca gagtattcta
     1861 taagagcact tccaattggt ggatatgtca gcatggaagg tgaagatgaa gaacaaataa
     1921 gtccaaattc ttttggaaac aagtctatat tacaaagatt tagtactatt gtagcaggac
     1981 caatattcaa tataatatta gcagcaatac ttttagttcc agtatttttg tatataggtt
     2041 ctccaacgac taaattagga aagataatgc cagatactcc tgcacaggct gtagggcttc
     2101 aagtaggaga taagataaat aaaattaatg gtaattctgt taagacttgg gatgaagttg
     2161 caaatataat aaatacttct tctggtggag aattaaaact tagtataact agagatggaa
     2221 gtgataaagt tgtaaatgta acacctaaaa ataacaatgg aaagtatgaa atagggattc
     2281 aaccacaaag agaaaaagac tttttagggt caatagtaaa tgcatgtaaa actactgtag
     2341 atatgacaaa acagatgcta acatttctag gtcaaatgat tacaggacgt gtaccaggag
     2401 ggattggcaa tgctgtagca ggaccagtag gtgttattgg tatggtatca gatgctgctc
     2461 gtacaggcat cataaatgta gtgtatttag cagctgtcat aagcttaaat ttaggtatag
     2521 taaatctatt accaatacca gcacttgatg gatggagaat acttatgctg ttactagaag
     2581 ctgttagagg tggtaaaaaa ctcgacccaa ataaagaagg catgataaat gttgttggtt
     2641 ttggagcttt aatgttattt atgcttttta tcacatataa agatatacta agattatttc
     2701 aatgatttag ttttttagag attttaagaa aataggctat gtagttttaa ataagaataa
     2761 attaattttt atgtatgaaa tttattctta tttaactaga tagcttttta atttataata
     2821 tacatgtagg ttataatatt ttaatattta aagagattca atataaaata atatatagag
     2881 gtggaatatg agttacgaga gaagaaagac gagagaagta agtgtaggaa ctgtaaaaat
     2941 aggaggagaa aatcctataa gtatacaatc tatgacaaat actgatacaa gagatgcaga
     3001 tgctacaata gctcaaataa agagattaga ggaagctggt tgtgatatag ttagagttgc
     3061 tgttcctgat attaaagctg ctaaaaatat agcaaagata aagtctagtg taaatatacc
     3121 aattattgca gatatacatt ttgattataa gctagccctt gaagctataa aacaaggtgt
     3181 ggatggaata agaataaatc ctggtaatat cggaagtata gagagagtta agatggtagt
     3241 tgagaaatgc aaggaaagaa atttaaaaat tagaattgga gttaatggag gttctttaga
     3301 gaaagaactt ttaaaaaagt atggctctgc aactgctgaa gcattagttg aaagtgcaat
     3361 gggacatatt aagattcttg aagacttaga ttttcataat atagttatat ctctaaaatc
     3421 atccgatatt tataaaactg ttgatgcata tgaattaata tcaaaaaaag ttgagtatcc
     3481 tcttcatatt ggaattacag aatctggaag tgttcacaaa ggaacaataa agtcttctat
     3541 aggggtagga gcacttcttc ttaaaggaat tggagatact gttagaatat ctttaacagg
     3601 cgaccctgtt gaagaggtta ttgttggtaa acagatttta agaagtttgg gacttttaaa
     3661 tgataagatt aaagttatat catgccctac atgtggtaga tgtaatatag acttaattag
     3721 tgttgtaaat gaggttgaag aaaagattgg tagtatggaa aaaaatatca ctgttgcaat
     3781 tatgggctgt gctgtaaatg gacctggtga agccagagaa gcagatatag gtattgctgg
     3841 gggtaaaggt gaaggtcttt tatttaaaaa gggtgaaata gttagaatga ttgatggtaa
     3901 caaattagtt gatgagttgc tagaagagat tgaaaaatta tagtatacaa ttcatatata
     3961 tattaattga ctagattttt aaatactttt gtaataagtt ttt
//
LOCUS       DAEMHZ010000224         4000 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000224 DAEMHZ010000000
VERSION     DAEMHZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.226"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..926
                     /locus_tag="KPK72_003705"
     CDS             6..926
                     /locus_tag="KPK72_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG2490871.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGNKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1041..2198
                     /locus_tag="KPK72_003706"
     CDS             1041..2198
                     /locus_tag="KPK72_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2490872.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2283..3782)
                     /locus_tag="KPK72_003707"
     CDS             complement(2283..3782)
                     /locus_tag="KPK72_003707"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG2490873.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEASMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         1576 a          554 c          609 g         1261 t
ORIGIN      
        1 aaaatatgta taaatcagaa aatttttcaa aaaaatctgt attaataaat tcatttttaa
       61 gtaaattttt aaaaataatt ggatggggtt tggtaagctt atttacatta tcagccttag
      121 tactttcaac tatttccatt ataattggat gggctgtaat acttcctatt caagtatgtg
      181 cagtattttt acttatagga ggagtttgta tttttaaagg taagagatta ggagaccaaa
      241 tttctcgtta taaaaaatat tgtacaatta taaacaatag aaatattatt ccagtagaac
      301 ttattgcaga aaacacatct aaatcattaa attttataat aaaagatgta caaaagatga
      361 tagacaaaaa ctactttata aatacttata ttgataaaag aaataatcag attgtgttaa
      421 caaatgaaga ttttattcca ccaagatatg aagatgtagc atatgaagtc aaagaagaag
      481 aaaagactga tgaatccaat gaagttgata caattataaa taaaggaatg aattatctaa
      541 cacaaataaa agaagctaat aaaaatatta aatcagaatc aatgggcaat aaaattgtac
      601 aagtagaaga tgttacaagt aaaatatttg atgttgtaaa acatgaccca tcaaaactta
      661 ctcaaataca aaaatttatg gattattatc ttccaaccac tttaaaacta ttaaattcat
      721 atcatacatt ggaagaacaa ggaattgata gagaaaatat aacaacaact atggaaagta
      781 ttgaaaatac aatgaatacg attgtagtag cttttgaaaa tcaattggac tatctgtttg
      841 aagatgaagc cattgatatt tctacagata ttactgtttt agagaatatg ttagtccaag
      901 aaggattaac gagtggtaaa ttttaacttc aatagaaaaa atattctgtt atttttaaac
      961 aattaattag tcataaagta taaaatgtgt attatataag ttaatcagta ctttatgaag
     1021 gaagaggaga gaaagataca atgaataatt tagatgatat accagttatg ccaactttga
     1081 cacttgaccc atttggagaa gaatcaaaca ctaatgatat agataattct gacttattaa
     1141 tgaaaaagga tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa cgcaagatgg
     1201 taaaggaatt tgcggaaaaa atagatatta caaatacaaa tatgattttg caatatggag
     1261 ctggtgctca aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt gtaaaaacaa
     1321 gagatttagg agaagttgga gatatgctta caaatctagt tactgattta aaagcatttt
     1381 cagcagatga aaaagaacaa agtggttttt taggaatttt taaaaaggca aataataaaa
     1441 tttcaaattt aaaaactaag tatgatagtg cagaggtaaa tgtggataaa gtaagcaaag
     1501 agcttcaaaa gcatcaggtt aaaatattga aagatatagc aatgcttgat aagatgtatg
     1561 aattgaactt agcttactca aaagagctta ctatgtatat tatagcagga aaacaaaaat
     1621 taaaggatat gaaagaatat gagatgccaa agttgagaga aaaagctcgt ttatctggtt
     1681 caacagaaga tgctcaatct gtaaatgata tggtttcact ttgtgacaga tttgaaaaga
     1741 aaattcatga cttagaatta actagaatgg tatcgcttca aatggctcct cagataagat
     1801 tggttcaaaa tagtaataat ctgatggcag aaaagattca atcaactata gtaaatacca
     1861 ttccattatg gaaaaatcaa atagtgcttg ctcttggaat ttctcactta aatcaagcta
     1921 tgaaagcaca gcgtgaagtt agtaatatga caaatgagct tttattaaag aatgcagaaa
     1981 ctttgaagat gggaacaata gaaacagcaa aagaatcaga aagaggaatt gtagatatag
     2041 acacaattaa gaaaacgaat caatctctaa tatctactat tgatgaggta gttaaaatac
     2101 aacatgatgg tagaataaag agacaagaag cagaagttga attatcaaaa atagaaaatg
     2161 aacttaaaag taagcttctt gaatttacag ttaaataaaa attatactaa tataaaaaga
     2221 gactgtctca aaatagagat aagtctcttt ttcattgttt aaatgattaa agtatattta
     2281 tattataaat ttctattatc ttttaacata acatcatgag ctccaccttc aactatacta
     2341 gtagaagata caagtgtaag tttagcattt ttttgaagtt caggaatgct aagtgctcca
     2401 cagttacaca tagtagaacg aactttactt aaagaaagag ttacattgtc tttcaaagaa
     2461 ccagcataag gcacataaga gtcaacacct tcttcaaaag aaagtttctt atctccacca
     2521 aggtcatatc tttgccagtt tctagcacga gcagaacctt ctccccaata ctctttcata
     2581 taactaccat ttatattaac tttgttagtt ggactctcat caaaacgaga gaagtatcta
     2641 ccaagcatta taaaatctgc acccatagct aaagctaaag ttatatgatg gtcataaacg
     2701 ataccaccat cagagcaaat tggaatataa atacctgttt tttcaaagta ttcgtctcta
     2761 gcttgagcaa cttcaattat agaagtagct tgtccacgtc ctataccttt ttgttcacga
     2821 gttatacata tagaaccacc accaatacca actttaacga agtcagcacc agcttcagct
     2881 aggtaaagga atccttcgcg gtcaactaca tttccagctc ctaccttaac tgtatcacca
     2941 tatttttcac gaatgaaatc aagtgttatt ttttgccact cagaaaaacc ttctgaagaa
     3001 tctatacata aaacatcagc accagcttca actaaagcag gaacacgttc agcaaagtct
     3061 ctagtattaa taccagctcc aactacatat ctcttagaag aatctaaaag ttcaagtgga
     3121 ttttctttat gagaagagta atctttacgg aataccatat acataagatt accattatca
     3181 tcaagtacag gaagagaatt aagtttgtgg tcccatataa tgttgtttgc ttcctttaaa
     3241 gtaacatctt tgttagcata aactatagat gacataggag tcataaattc actaacttta
     3301 gtatctaaat ccatacgact tatacgataa tcacgacttg caacaatacc caataattta
     3361 ccagttgaag ttccatcttc agtaacagct actgtagaat gtccagtttt ttcttttaag
     3421 tcaagtatgt cttttaatgt gttatcaggt ttaatatttg aatcacttac aacaaaacca
     3481 gctttgtggg atttaacacg tgctaccata gaagcttcat tttcaatagt ttgagaaccg
     3541 tatataaatg aaataccacc ttctttagca agtgcaatag ccattttgtc atcagaaaca
     3601 gattgcataa tagcagaagt caaaggtata ttcatataaa tatctgcttc ctcgcccttt
     3661 ttaaatttag taacaggtgt ttttaaactt acattagcag gtatacaatc agtagaagag
     3721 aaacctggta caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa ataaaatgcc
     3781 attttttaat cactccttat atttttatac tttttataat tgatagtatt aattataact
     3841 tattctttaa ttttattaaa gtattttgtc aaatattgag atttttgaaa aatttaaact
     3901 attttaatta aatttgttta atatatattg ccttataggg taaagttgta tattaaataa
     3961 tgtttagtgt ttaatagtag ctaatttaaa attaatcttt
//
LOCUS       DAEMHZ010000225         3913 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000225 DAEMHZ010000000
VERSION     DAEMHZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.227"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1707)
                     /locus_tag="KPK72_003708"
     CDS             complement(<1..1707)
                     /locus_tag="KPK72_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490874.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNKQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAILLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGAVCLILGYAMIIF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGESSKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKSYNKLSKELGLDKISSKLERYEGIII
                     PSSISQEHINSLKQLKNYNIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIP"
     gene            complement(1707..2453)
                     /locus_tag="KPK72_003709"
     CDS             complement(1707..2453)
                     /locus_tag="KPK72_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490875.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVILDGYDVSKLEGEKLAEFRRKQLGFVFQDFNLVDTLTVG
                     ENIMLPLTLDGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNIDSKSTDDVLKLFTRINKEQNVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(2600..>3913)
                     /locus_tag="KPK72_003710"
     CDS             complement(2600..>3913)
                     /locus_tag="KPK72_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2490876.1"
                     /translation="KLIEQLKKLAYYDSLSGIKNKEKFRKDSMYILKNYYQDNFYLVQ
                     LDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDHFILLIACNTEDE
                     LLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIAAKSKK
                     EKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEIEGAEA
                     LIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPVPISVN
                     MSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKKIKEIGFL
                     ISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKAIDIKV
                     ICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
BASE COUNT         1369 a          640 c          353 g         1551 t
ORIGIN      
        1 cggtataaaa aacaatgtac ctatctctat actaacaatc ttattcatat ttttaaaaga
       61 tagacctatt tttgatagtt ttttgtactt tacattatct tctatctcgt ctgtatataa
      121 tctaaagtat aaaaaactaa aaactgctat aaataatatc actcctataa aaacacccat
      181 aaattgaaga attttacttt gttgtaactc tactaaatac atatctggta atgttaaaaa
      241 atatgacctg ttatattctc tatccaaatt aaaatgtttt tcttttaaat tttcagttat
      301 ctttgcactg ctttgccagt ttttataatc aaatccatga aaattaattt ttgaaaattc
      361 accttttact tcatcgtata agtcatcact tataactaca actttactaa acagtcctgt
      421 aggaaaaatt attttatttt ttgatttttt tacttctata tttaaatttt ttatattata
      481 attttttaat tgttttaaag aatttatatg ttcttgtgaa atgctactgg gaattataat
      541 tccttcatac ctttctaact ttgaactaat tttatctaac cccaattcct ttgataattt
      601 gttataactc tttacactta gtaagacttc cctattatat ttaagtacag gaaaacttaa
      661 ctccttataa tcatatcctt tgattgcttc tctaatttgt tgtagttctt catattcttt
      721 ttcattatta tgctctgaaa aatatgagta aaccattggg aaattatttt tagaagattc
      781 tccttgagag cttacaagtg ttgaagttgt tgtaaatgct actaaagtag ccgataaaag
      841 tattgttatt aaaaataaca atcgtgtatt atcctttaat ttatatatca aattacttat
      901 aagtaacata tttactttgt tcttataaaa tttttcttta ttttttaaat actttaatat
      961 caaaatagtg aattgagaaa aaaataaaaa tatccccaaa gtaattaata gtgctgttgt
     1021 atacatacct ccatttattt ttaacttatt gccaaatatt atcatagcat atcctaatat
     1081 aagacacact gcaccaatta ttccaaatag ctttgaagat tttatctctt ttttaggctt
     1141 ttttgtacct actaaaagct ctaaaatact ctttttactc aataatttta agctcattgg
     1201 tcctgttatt aaaaacaaaa tcataaatga tacaatagtc aatagtattg cttttactgg
     1261 aaaataaaat tcagttaaat tcatataaaa cagtttgctt aaatacaata aaaacaattt
     1321 tgaaattgat aaaccaagta atattcctaa catcactgct aaaaacccta ttatcaaatt
     1381 ttcaattaat atcaattttt tcagttgttt gttgctcatt ccaattatca tcaatactcc
     1441 aaaatctttc aatctatatt ttaagaaatt tcctaaagag tacaatataa aaagtagact
     1501 aaaacctaat attattaatt ctgaagtcat aagagccata aatgcagtag aaccactctg
     1561 cattccagat tttgttataa tcggatggaa catagacact gcaaaaacaa aaaatatcat
     1621 tatagaaaaa acagaactta aaaaatatgc taaatatgcc ctgaaatttc taactatatt
     1681 attataagca aactttctaa aatccactat cttacacctc ctatttgaga caaaacagct
     1741 agtatatcag aatagaaaga attactgctt tcagattttt ttatttctgt atatatacaa
     1801 ccatctttta taaaaattac tctatctgaa taacttgcac tatatgcttc atgagttacc
     1861 ataagtgttg ttacattttg ttctttgttt atcctagtaa ataatttcaa aacatcatca
     1921 gtagattttg aatcaatatt tccagttggc tcatctgcaa gaagtatggc aggtttattt
     1981 ataatagctc tagcaatagc acacctttga gcttgccctc ctgaaatttc ataggttttt
     2041 ctatctaaaa ttttatctat tcctaaaaat ttagatatac ttttagtttg tatattcata
     2101 tctttgatac ttgccccatc tagtgtaagt ggcaacataa tattttcgcc aacagttaaa
     2161 gtgtcaacta aattaaaatc ctgaaataca aaaccaagtt gtttccttct aaattcagca
     2221 agtttttctc cttctaattt acttacatca tatccatcta atataacctc accttcactc
     2281 tgtctatcaa ctgtagatat tatatttaaa agagtagatt taccacttcc agatggtccc
     2341 ataacagaca caaattctcc cttttctata tttagactaa ccttatctaa tgctttaaat
     2401 ggaatatttc cacaatatgt ttttgatata tttctaatac tcaatatgtt cattttatat
     2461 tctccataat tttttatttt tactacaata ccataatatc gtgaatatac tactttaacc
     2521 ataaaaatac cctaacaatt aacttacata tttgtaagta agttgttagg gtatctcata
     2581 gcctaagaat tattaatgat taatcaaatt taaagtttaa gtgcttttca aattcatcta
     2641 ataccatagg tttagcaaat aagtatcctt gaaccttatc acaacctatc tctttcaaga
     2701 actctacttg ttcataggtt tctacgccct cacagattac tttaatatca atcgcttttg
     2761 ccatttccac aatcttagaa ataataattt ttccacgttt tgtatctgtt gtttcgatta
     2821 aaaatcctct atctaatttt aaaatatcaa aagaaaggtc ttttaacaga tttaaagatg
     2881 aatatcccga accaaaatca tccattgata taaggaatcc tatctcttta atctttttca
     2941 ttatatcaat caatatattt aaattatcaa atacaacact ttctgttagt tcaagttcaa
     3001 taaactcagg agatatattg tatttactta tcaaatcagt tatattttct ataaaattat
     3061 ccctatataa atgaactcta gacatattta cagagattgg tacaggagta taccctttat
     3121 ttatccactt gtttaatgtt ttgcatactt cttcaaacac gaacatatca atatttacaa
     3181 taaaaccatt cttttcaaat aaaggtacaa aatctatagg tgaaataaac ccaaattctg
     3241 gactattcca tcttatcaag gcttctgctc cttcaatttc atttacatca tctaaactat
     3301 acttaggttg ataatatact ataaattcac ctttttctaa agctttattc atattatctt
     3361 ccaatcgttt ttccttgtac aatcgatttc ttgtatcttc attaaaaaat gaatatgaat
     3421 gttcatactt ttccttttta ctttttgctg caatatttgc tctatcaatt aacaagtcaa
     3481 tctttttaat atcatccttt ttagtaatct tatatattcc acttgacata acaattttat
     3541 acttagatga gttagtagta tttaagttac aaatttcttt atttatttta gataatctat
     3601 ttaaaagttc atcttcagtg ttacaagcaa tcaacaatat aaaatggtca ttatccatcc
     3661 tagcacaaat ttcatattta tttgtattat tatttaaaac ctgtgatata tgaattaata
     3721 tcttattccc ttcagcatat ccaaacatct catttatata tttaaattta ttgacatcca
     3781 attgtactaa gtaaaaatta tcttgataat aattctttaa aatatacata gaatcttttc
     3841 taaacttttc tttatttttt attccactta aagaatcata ataagccaat tttttaagtt
     3901 gttctattaa ttt
//
LOCUS       DAEMHZ010000226         3892 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000226 DAEMHZ010000000
VERSION     DAEMHZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.228"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(197..577)
                     /locus_tag="KPK72_003711"
     CDS             complement(197..577)
                     /locus_tag="KPK72_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2490877.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(778..1971)
                     /locus_tag="KPK72_003712"
     CDS             complement(778..1971)
                     /locus_tag="KPK72_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2490878.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIVGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKIHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            complement(2034..3290)
                     /locus_tag="KPK72_003713"
     CDS             complement(2034..3290)
                     /locus_tag="KPK72_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2490879.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1412 a          721 c          407 g         1352 t
ORIGIN      
        1 tagagatgac atatcaaaat atttttaaaa agtaatttaa gtattagaag taattgaagt
       61 tagtaaatat aaatgcgttt acttttaagt attgcttaag ggaataaaaa ataaaaataa
      121 actctgcaac catctaaaaa tagttgcaga gcttacaaaa ccaaaattat ctaaattgta
      181 ataaaaataa taacatttat acataagctt cttcccaatt tactacccag ttttctaacg
      241 actttatctt ttcctctgtt agctcctctt cgtgaaatga tactaataaa ttccttataa
      301 aattattctt caaaagattt tgcacatttc ttgatataaa ctctaaatat gccttttctt
      361 ttattgcaac tgtataatac atacacttgc ctgtttcttg aacatataag aatcttttct
      421 ttgatagttt tgataaagtt tttaaagcta ctttttctgg taaattatgt tcttctttca
      481 tataatcaat aatctcatat gaattggttt ttatatctaa attccaaata aatttcataa
      541 taactaactc taattttgat aattttttta aacacatagt tatcactccg cttctttatc
      601 tatataatta aatattacaa taataatttc acaaaatcaa ttctaataat cataacatac
      661 cttaaaatct gttcttaact tctaccttct taatctccca aatgattttt ttatattgta
      721 ttcaatagta taatcctttt ccaaaatata acttaccagt taaaataatc tttattttta
      781 cttattctcg ccttttacag aagttaataa ttttttataa gttacatcaa ttccaaaagc
      841 tccaagaggt gcagttataa gtatagctag aactgctaac gtcaaaataa gttgtccaga
      901 tgccatcccc attgcaagtg gtacacctcc tattgctgcc tgtactgtag cctttggaat
      961 atagccaatc atacagaaca atctttcctt tattgtcaaa tttgtcttaa taaggcagaa
     1021 gaatactcct accatacgga ataaaagaac tccaaatatt aaaattacag ctccaattcc
     1081 tgctttaaat gcataactta tatctactgt tgcccctacc aaaacaaaca agagaatttc
     1141 agcaccaacc catagtttcg aatactttat agacaatctt cttgcaacta cacgatattt
     1201 tatctgaagg aaaatcccaa tactcataac tgctaataat ccagaaaatg gcacaatacc
     1261 ctctaaagca gtctctaaac ttatcattaa aaatgagata cttaaaagca acactccttt
     1321 agcagaatct ctcatatgaa tcttcttaaa aaatactgct aaacagattc caatcactgc
     1381 tccaactatt acacctaata taattgatac tggaatttgt aaaaaactaa tagctgatat
     1441 actatttcct tgtgccaatc ctgtgaatac tgtaaacatc acaattacga aaatatcgtc
     1501 tatagaagtt cctgctaaaa gcatctgtgg aatacttttt tctgtaccat aaccttcttc
     1561 cattaattta agcattttag gtacaataat tgcgggagaa actgctccta ctactgcacc
     1621 catgactgct gcttccaata tagaaacgcc tagtaacttt ggtgctaaaa ctatcattcc
     1681 gacaatttca aaagtagctg gaataaagca cataagtaca gcgggtcttc ccactttctt
     1741 caaatcgttt atatctaaag ataatccagc ccttgttaat ataataatca atgcaatctt
     1801 tctaaggtca gaagaaatat ctagtattga attatcaatc aaatttaaaa catatggacc
     1861 taatataatt cctgtaagta acattcccaa taagttaggt aattttatcc tcttaaaaat
     1921 acctcccgag gccattccta ataaaaaaat caaagctaaa cttgtcaaca tattcctacc
     1981 ctatcctttc tctaatattc tttctcttct aatcatacat tatgttgttt tatttattgt
     2041 tttgttgaaa ttttttgtag tgaataaata gatttatcca tattaaatat atatgaaata
     2101 gcgcatacta caaagaaata aggtaaatag ctgtacccaa aaacttctgc ccctataaat
     2161 acaggtgcaa aaaaagtatt tgtagcacta ccaaagacac tagcatagcc aagcgctgca
     2221 actaattcaa ttggcaaatt aaaaaatcct gctaacaata caccaagact agctccaatg
     2281 gaaaacaaag gtgtaacctc tcctccttga aaacctgctg acaaagtcag tattgtaagt
     2341 ataaacttta acagccagtc aaatgaataa atttctccac catagaaact attttgaatt
     2401 aaatttgtcc ctaaacctga atatcttcct ttataaaata aaaggaataa tacactaaga
     2461 catactccaa taatagcaat ccttatcata ggatttttta atctattccc tatttttctt
     2521 tttgacaatt tcagacacca tgcaaatgct ccaccaacca ttccaaaaat aattcctaaa
     2581 acaataagct tccaaaatat tgacaagtca aatacaactt tagaagaaag tgcaaaagaa
     2641 aatttctcta accctaaagc ttttgatact gcacttgctg taaaagaagc agtaaacgca
     2701 ggaaaaagcg attgatactc cagtgaacca gcaaccaaaa cctccatagc aaatagtatt
     2761 gctgctattg gtgtctcaaa taatcctgca aaaccagctg ccataccagt tactagaaaa
     2821 atactagaag cattttttat aggcaacctt ttacctaccc aatgtgaaaa agttgcacca
     2881 atctgaacag caactccttc tcgtcctgca ctaccaccaa ataaatgggt taaccatgta
     2941 cctgatatta taaatggaac caatcttaaa ggaataattt cttcttctcc atgccctacc
     3001 tcaaatatta gattcattcc tttactactt tttcctccaa atttaaaata gcaataagca
     3061 ataacaactc ctactactgc taaaaatggt atcagataca ttggatatgt ttctcgaata
     3121 tctgtaactt ttagaagaac cgttccaaat atggtatcta taagacctat aattgcacca
     3181 actggtatac ctatgagtcc taaaaaaaat agtccaccat atgttttata aacgctttta
     3241 attcttatta tcattttaat ccatattaaa gttttaaatt tttctatcat tttaattcct
     3301 ttctatttgc atcataagat tttttgaaag ttttattaca tcaactgtag tcaatgtaat
     3361 gactatcata attgtttttt aaattgatag gatataggta cactcttaac ccattcttaa
     3421 taaaataatt aacatataat ctactatttt ttctattaat taaatatttc ttcaaattta
     3481 aaaaataaaa atttagtaag taattcacct ccattttatc gatttaaaca acaaaaaaag
     3541 ctggtggcat ctatgatatc atagaagtca tcagctcatt ggcagtttag gatattatca
     3601 tccaagggag aacctcattc ccataattat tatataattt tttatttttc atatgtctat
     3661 tttatgtaaa ttgtattttt aatgttataa ataatataac ctatcaatta aaatagtaaa
     3721 gcctctaata ttattagtgt tcaatatttc atattatttt tatgatatat aatttaaatg
     3781 attttcttta ctttgcatta tgatacatga tttaaatatt tttcctcact ccacattatg
     3841 atatattatt taaatatttt tcttcactct gcttatatta tgatacataa tt
//
LOCUS       DAEMHZ010000227         3812 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000227 DAEMHZ010000000
VERSION     DAEMHZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.229"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..605
                     /locus_tag="KPK72_003714"
     CDS             <1..605
                     /locus_tag="KPK72_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG2490880.1"
                     /translation="KYLIIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKYM
                     FNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNI
                     NFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRI
                     KALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            559..987
                     /gene="ytfJ"
                     /locus_tag="KPK72_003715"
     CDS             559..987
                     /gene="ytfJ"
                     /locus_tag="KPK72_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG2490881.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1235..1675
                     /locus_tag="KPK72_003716"
     CDS             1235..1675
                     /locus_tag="KPK72_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2490882.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1691..3142
                     /locus_tag="KPK72_003717"
     CDS             1691..3142
                     /locus_tag="KPK72_003717"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG2490883.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3233..>3812)
                     /locus_tag="KPK72_003718"
     CDS             complement(3233..>3812)
                     /locus_tag="KPK72_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG2490884.1"
                     /translation="NILPYSYTVEKIICTLLVIIMALEQLLLISSGNYSTLNSLPIGI
                     NYICIYLCIAILIFKQYHLFNIFFSWSLVCSVGELIFSKNLGYEFPSLIYFIFIFSKC
                     LIIYADIYMVDVRKFRVNRYALRDNLAICFIYFSFIFLLNTFTNSRYYYGFLSHSTTA
                     IFTFIFVTSIMYIPALLFNRDTFILEKKKKSK"
BASE COUNT         1585 a          346 c          660 g         1221 t
ORIGIN      
        1 ttaagtattt aataatagct gtaattttta ttataagtgt aactatgttg attttaatat
       61 ctcatataaa catcttaata atagctgata taaaaaacaa agatatttgt ttaaagctta
      121 atataaaata catgtttaat ttaataaata taaatagaca actttatcca gctgaaaata
      181 gtaaaaataa tgataaaaaa gaggggatga agaataatat agattcaagt atattattag
      241 ctgatgacct tttgagcatt tatagattat taaaaaagat aaaaatacat gaactttatt
      301 caaatattaa ttttggaact ggaaatattg gcttaacatc ttctgtatat gtattgataa
      361 atactttgta tggaaattta tttaatatga ttgatgcaga aaaaatgtat ttaaatgtaa
      421 atccagattt tacaaaagat tatgttttag gaaatataag gatacatata aggccaagaa
      481 taaaagcttt atttaatatt ataataatga ttaataaaat tatgaataaa aataaaggaa
      541 ataaggaagg tgatagtaat gagagcaaca ggttcgatac agagtcttat ggaaacaact
      601 cttgaaacaa ttaaaggttc tatagatgca aatactatta taggagaccc tataaaaaca
      661 gatactacag tggttgtacc tatatcaaag gttacaattg gctttggtat aggaggagga
      721 gaatactcaa aaggatatga tgataaagat agggaaattg aattaaaaaa tgaaaaatca
      781 gatacgaatt ttgctggagg tagcgcaggg gctatatctg tacaaccagt agcatttgta
      841 gtagttgaaa gtggagagac tagaataatg agtttagaca gtaatatcaa tctagtggat
      901 aatatattat ctataactcc tagagtattg gagaaaattc aaaatatatc acaaagtaat
      961 aagaatcaag ataaaaacaa tatgtaagaa taaaatattt ttaaatatgt atatagtttt
     1021 aggtaaagta gtggggttag tataaatccc actattttgt tgtgtacttt aaacatttat
     1081 atctaataag attttagttt aaaacaattt aaatagataa taaagttgat atatagtata
     1141 attataaaaa gaaaagaagt attatattat agatatataa acgcatttga gatataagag
     1201 ggagttataa atcattagtt ttgaggtgaa tgttatgagc gataaagaat tatctatttt
     1261 gttgttagaa agtatgaata attttcataa tttaataaag attattaata acgaaagata
     1321 taaaaaagat aaggttctta cagaaagaca gttctttgcc ttagtaaaaa taagaaaaca
     1381 tgataaaata gagcttaaaa atctaagtag agatttgcat gtatctactt ctagtctgtg
     1441 tatactactt aataaattgg ttgagcaaga gtatgtatat agggaagaag atagtagaga
     1501 tagacgaaat actttttatg gaataactaa aaatggagaa aagatattgg ataatgaaat
     1561 actaaagttt gtatctataa taagtgataa aatggattgc ctagatatag ataataaaga
     1621 taaattattt acttctttgg aagagtctaa aaatataata gaacaattat tttaaaaagg
     1681 agaatttatt atgaatataa agtattctaa agaaatatta aaaagtttag aaaatgctat
     1741 aacagtagta gctctatttg agggagaaga tgtttctacg acatttgata aaataaatat
     1801 gataagcaga aattttaatt ctaaagaaaa tagtatagat actgtaacat tgattgaaaa
     1861 taacaatttg gtaaatgttt gtcttgttgg ttttggtaag agagagttat tgaatagaga
     1921 aaaaattaga atcataggtg gtaatttgtg taaaaaatta aaaaatatat tgaaaaatga
     1981 agcatataag agtagtgatg ttaatgtact gaatcttaat ttaaatagtg atgaaatagg
     2041 ttcttttgta gaagggatat tacttggaaa ttataagttt gataagtata agacaaaaat
     2101 taaggatttt gaaagaaaag aagttgatac tataactata tttactgaaa aagaagttga
     2161 gtgtcaaata gaaaaggcta aaatattagc taattcaact ataatagcta gaaatctagt
     2221 taatgaacca tcaaacgtaa tatatccaaa aacattagca gtagaaacag ttaaacttgg
     2281 tgcagagttt gggtttaatg ttgatgttta tgaagaagaa aaaataaagg cattgggaat
     2341 ggatgctttt ttcgctgttt caagaggctc aattaataaa ccaagattca tagtaatgag
     2401 atactttgga gataaagaaa gtacagatat attaggtcta gtaggtaaag gattgactta
     2461 tgatactggt ggatattctt taaaatcaaa tgcttctatg ctagatatga agacagatat
     2521 ggctggtgct gcgagtgtta taggtgctat gtgtgctata tctcaaagta aattaaagaa
     2581 aaatgttata gcagtagtgg ctgcatgtga gaatgcttta tcaggaggtt catacaaacc
     2641 tggagatata attagttcta tggcaaagaa aactattgaa gttttaaata cggatgcaga
     2701 aggtagactt acattagctg atgcaatata ctacattata aacaatgaaa aagtaacaaa
     2761 ggtggttgat gtagctacat taactggtgc tgctttaaca ttgttaggta atgttgcaac
     2821 tcctatagta acaaataatg atgattttta ttgtgaatta gaaaaagctg ccactttatc
     2881 aggggaaagg gtatggaaga tgccaattta tgatgaattt aaagatatga taaaaggaga
     2941 agaagcagac cttaaaaaca ctggtggtaa aaatgctgga tgtataacag caggagcttt
     3001 tattggtgaa tttgttggga atactccttg gatacatatg gatatagcgg ggacaagtac
     3061 atcatcaaaa tcaattggat acaaagcaaa gggggctaca ggagaacctg ttagaacatt
     3121 gtactatttg gcagaaatct aaaaaacaat tataaaatat aatttataaa gccacgtgga
     3181 tatttttgta atttaagatt atctatgtgg ctttattttt tatttaaata aattatttac
     3241 tctttttctt tttttcaagt ataaaagtat ctctattaaa taaaagagca ggtatgtaca
     3301 taatactagt aacaaaaata aacgtaaaaa tagcagttgt gctatgactt aaaaaaccat
     3361 aatagtacct agagttggtg aatgtgttta gtaaaaatat gaatgaaaaa taaataaaac
     3421 atatagctaa gttatctcta agtgcatatc tgttaactct aaattttcta acatcaacca
     3481 tatatatatc tgcataaatt atcaagcact tagagaagat aaagataaaa tatattaagc
     3541 taggaaattc atagcccaag tttttagaaa aaatgagttc tccaactgaa caaactaaac
     3601 tccaagaaaa aaatatatta aaaagatgat actgcttaaa aattagtatg gctatacaca
     3661 gatatataca aatataattg attccaattg gtaaagagtt tagagtacta taattacctg
     3721 aagaaataag taataattgc tctagagcca taattattac aagtaatgta caaattatct
     3781 tctcaactgt atagctataa ggtaaaatat tt
//
LOCUS       DAEMHZ010000228         3648 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000228 DAEMHZ010000000
VERSION     DAEMHZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.230"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            210..1103
                     /locus_tag="KPK72_003719"
     CDS             210..1103
                     /locus_tag="KPK72_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG2490885.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1103..2497
                     /gene="gltA"
                     /locus_tag="KPK72_003720"
     CDS             1103..2497
                     /gene="gltA"
                     /locus_tag="KPK72_003720"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG2490886.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            2863..>3648
                     /locus_tag="KPK72_003721"
     CDS             2863..>3648
                     /locus_tag="KPK72_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG2490887.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTN"
BASE COUNT         1436 a          377 c          718 g         1117 t
ORIGIN      
        1 acttagtgtt tattggcatt tacaagaaat ttgttaatat caactatttg tagtttgggc
       61 agttataatt attatagggg ggatgtccaa tacaaaacaa tcaaccaaga actaatatta
      121 tattataaag agttatagtt ttgtgtgttt atgtttttaa ttaaatttaa agtgggaaaa
      181 atatatttgt tagaatggag aatagcgaga tgagtaataa aatagttagt aagagacaat
      241 taacagatag tatttattta atggaaatag aggcacctag agttgcaaaa tcttctcaac
      301 caggtcaatt tataataatt aaaaatgatg aaaaaggaga gaggattcct cttacaatag
      361 ctgactatga tagagaaaaa ggaacagtaa ctattgtatt ccaaacagtt ggtgcttcaa
      421 ctaaaaagtt agcaatgttt gaagaaaacg attttgtaat ggattttgta gggccattag
      481 gacaagcaag tgaatttata catgaagata tagaagaatt aagaaataaa aagatattat
      541 ttgtagctgg tggagttggt tctgctccag tttatccaca agttaaatgg tttaaagagc
      601 atggattaga tgtagatgta ataattggtg caagaactaa agaattaatt atattagaag
      661 atgatatgaa aaaagtagct aaaaatgtat atgtatcaac agatgatggt acatatggat
      721 ttaatggaag agttacagac ctattaaaag atttagttga taatcaaggt aaaaaatatg
      781 accaagcaat agtaattgga ccaatgataa tgatgaaatt tatgtgccaa ttaactaaag
      841 aactaaatat acctactata gtaagtttaa atacaataat gatagatgga acaggtatgt
      901 gtggtggttg tagagttagt gttggaaatg aaactaagtt tgcatgtgta gatggtcctg
      961 aatttgatgg tcatttagtt gattttgatc aagcaatgag aagacagtct atgtacaaga
     1021 ctcaagaggg aagagctatg ctaaaacttg aagaaggcga tagtcatcat catagtaatt
     1081 gtggatgtgg aggtaacaaa taatggatgc taaaaaagta aaagtaccag ttagagaaca
     1141 agaacctgct gtgagagcaa caaattttga tgaagtatgc ttagggtata ataaagaaga
     1201 agctatggca gaagctaata gatgtttagc ttgtaaaaaa ccaaaatgtg tgggtggatg
     1261 tccagtagga atcgacatac cagggtttat aacaaaaatt aaagaagatg atatagaagg
     1321 tgctgctaaa gtaatagcta agagcagttc attaccagct gtttgtggta gagtatgccc
     1381 acaagaaagt caatgtgaag gtgtatgtat acttggaata aaatcagatg ctgtatcaat
     1441 aggtaaatta gaaagatttg tagcagattg gtcaaaagaa aatgacataa atttatctga
     1501 tacagagcct aagaaaaatc aaaaagtagc agtaatagga agtggtccag caggtctagc
     1561 ttgtgctggt gatttagcta aaaaaggcta tgatgtaaca atctttgaag caatgcatga
     1621 gccaggtgga gttttaactt acggaatacc tgagtttaga cttccaaaac aagctgttgt
     1681 tcaacctgaa atagacaata taagaaaatt aggtgtaaaa atagaaacta atgtaattgt
     1741 tggtaaaaca attacagtag atgaacttat agaagatgaa ggatttgaag ctatattcat
     1801 aggttcagga gctggtcttc caatgtttat gaatatacca ggggaaaatg caaatggagt
     1861 gttttctgca aatgagtttt taactagagt aaacttaatg aaggcttata gagatgacta
     1921 tgatacacct attagttctg gtaaaaaagt tgctgtagta ggtggtggaa atgttgctat
     1981 ggacgctgct agaactgcac taagattagg ttcagaatca tatatagttt atagaagaag
     2041 tgaaaaagag cttccagcaa gagctgaaga agttcatcat gcaaaagaag aaggtattat
     2101 atttaataca ttaacaaatc ctaaggaaat tttagtagat gaaaatggat atgttaaagg
     2161 tatggtttgt ataagaatgg aattaggaga gccagatgat tctggaagaa gaagacctat
     2221 agaaatagaa ggttctgaat ttgtacttga tgtagacaca gttataatgt cacttggaac
     2281 aagcccaaat ccactaatat cttctacaac aaagagtctt aatataaata agaagagatg
     2341 cttaataaca gatgaaaatg gtcagacttc taaagagggt gtatttgcag gtggagatgc
     2401 tgttactgga gcagcaacag taatatctgc aatgggtgct ggtaaaacag cagcagcatc
     2461 aatagatgaa tatttaaaag caaaagttaa tgcttaattt gtaaattaaa gagagggtgt
     2521 ctcaaaatga actttttagt tcatgaggca ctttttttgt acaaaatcaa atatgtttta
     2581 atcatttcaa caatgaaaat ttcattcctg tagaaagtct aacttttaag tcttagtaca
     2641 atctctattt tgatgtagcc acttttttag atggtttaat aatttatcag ttattattgt
     2701 aatatttgat actaagtaac ataactttgt ctaactatga taaaatagtt atacaataaa
     2761 ggtcttgtaa ggagtgaata atgtgtggct aataaaatca gatttgtcaa atctaacatt
     2821 aaataataga aaaataaatt gtgtaattaa tggtatttta atatgaaaaa aagagaaaaa
     2881 gtatttgatg ggctagtttt taagcatata ttaatcttat taatactatt agttttgatt
     2941 gatttaacaa gcaccatcat atatgcaagt aactctatgg aaattacttc tagaaatatg
     3001 attgaggcat caaaaagaga acttgaaaac tatttagaag taaacatatc tttattaaaa
     3061 gctcttagtc aagatgatag attttctaat ggtgaaacat cactaattga aaagggaaaa
     3121 ttattaaggc catatcaaaa ggaatataat ctttttatga ttggtataac agatacaaaa
     3181 ggaaatgctt caagtacata tagggaaaat gttaactcaa taaaagataa gcctagtttt
     3241 gagaaagcaa ttaaaacaaa gcaagtagta gtttcagata ttgaagtcag taatgtaaca
     3301 ggtgacaaag tatttatcat atatgtacca attgtaaaaa ataatgagat gattgggacc
     3361 atttttgctt ctttttattt tcaagatgta aataatttaa taagtagaag caattttgat
     3421 gattctatta aatttctaat gatagataaa gattatacta ttatttctca tccaaacaaa
     3481 aaatatgtaa atgataaaag taagatgctt gatttagaag ggaatattat aggaaccact
     3541 aaaagtgaaa tcttaaaaaa tataaataaa aaatgtcaag gtgatttttt atcttgggat
     3601 aattggagat tatataatgt aaaatatacg aacataaagt ggactaat
//
LOCUS       DAEMHZ010000229         3629 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000229 DAEMHZ010000000
VERSION     DAEMHZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.231"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   56..811
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=122..150
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1470..3563)
                     /locus_tag="KPK72_003722"
                     /pseudo
     CDS             complement(1470..3563)
                     /locus_tag="KPK72_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         1266 a          579 c          354 g         1430 t
ORIGIN      
        1 atatagttaa tataaaacga tttcaaatga ttagaataaa aatatcagaa ttgctattta
       61 tattccatat agttaatata aaacgcatta tatggctgtg tcattttgca ttctattaca
      121 tatttacatt ccatatagtt aatataaaac ctgcttaatg cagaattaag agaggaagtg
      181 ttataagatt tacattccat atagttaata taaaactggt tgttcctgct ccggtgttgg
      241 tggaactggt acatttacat tccatatagt taatataaaa cgaatatgta tggataattt
      301 tttacttaac atattagcat ttacattcca tatagttaat ataaaacctg ctgtaatttc
      361 tgatgaagct tctccactac caccatttac attccatata gttaatataa aacgtacaaa
      421 ttttcgtgtt gttgcagatt atttatatcc atttacattc catatagtta atataaaacc
      481 cttctttaac tcttatcgta tttaaacctc ttataacatt tacattccat atagttaata
      541 taaaacatac atgtctatgt tgctataagt ttttaattca ctaatttaca ttccatatag
      601 ttaatataaa actttttgtg ttaagaagaa aaaagaaatt ttgataatat ttacattcca
      661 tatagttaat ataaaacctt aaagaaaaaa tggaggaaga ttaagtgaaa aaattattta
      721 cattccatat agttaatata aaactggatt taaataatca aaacaactcc ggtttaaaaa
      781 acatttacat tccatatagt taatataaaa ccccaaaata aacttagtat tttcaacact
      841 tacacgtata caactctctt aaatttgcag tgaacgagca atagtgcaat tgataacgct
      901 tatcatacac tctcaacgcc ttatatttca actgttacgc caaatttttt cacaaatatc
      961 gctcactgca aaattcctat aattttatta tatcataaat atattacttt tgaatattga
     1021 taccaattta taccataata aaagcaaaaa aacgtaatct atctacatta gatttaaatt
     1081 ctataactta aagtttttta cataatattt atgcttgttt aatcaatcta atatatcatt
     1141 cttgtatatt gcttttaata gtcttttttc ttaaataaat atctatctga acaagcatag
     1201 tttaattaaa aacttttgta ttcccttaaa actgtatcta cctcttatta ttttatttat
     1261 gatatattat ttttaatcta aaaatagata tctattttta ttatggttat ccctttttaa
     1321 aatactttaa tcatatacta ttcccaattt aacatccatt gaaatgctat tttcttatat
     1381 tgcaatttat aattgatata tcttcaatat aataatttaa tgtatttttg attttaaagc
     1441 ttttcagtga tattattgat aaattagaat taattatcta gctttaaaag gtcaaagatt
     1501 tctgttgctg tctgcggttt tccaaataaa tatccttgta tgtaatctgg ctttatttgt
     1561 ttaactaaat catactcttc ttgtgtttct acaccttcta aacatacttt tattcctacg
     1621 tcatgacaaa ttgcaacaat aaaatgtata aatgtagcat caaacttact ctttaatata
     1681 tctttaacaa atactctatc aattttcact atatctattg gagaaaattt taaaacttct
     1741 agagacgagt atcccgtacc aaaatcatcc atagcaatat aaattcctaa atcttgtaaa
     1801 gccttaaatt taaactgtaa aatactcata ttttgtactg tatgactttc tgtcaattct
     1861 aaaacaagat tttgataagg aaaattttca tcatctataa tttttactat atcttcaata
     1921 aatgtgtctt ctaatatttg aacagctgaa acgtttacac tcactttaaa aaaaggatag
     1981 taatcaatcc atttcctaaa agtacgtaat gccattcgaa gcacccaagc ccctacaata
     2041 tttatcatat cattttcttc taatattgga ataaattcca aaggagaaat tgctttgcat
     2101 ttgtcattag tccaccttaa aagaacttct acacctatta ttttatgact ttctgtatct
     2161 acttgtggct ggaatcgaag actaaacccc ctaaaatcat cattaattga tgcctttaaa
     2221 tcccgcatca tttctaatga atacatttta ttttttaata tttcttggga gaaaaaaaca
     2281 agcctgtttt taccatgctc tttcgcatat tgtaaagagt aactagcaca cttataaagt
     2341 tcttttactg tatctccatg ttttggatat attacacaac cagcagaaat tgttatattt
     2401 aatccatatt ttctccatag ttgaaggtgt ataatcatat tctgtatttg attatataga
     2461 gttagaattt cattttcttc aacattatca acaagaatgc ccatttcgtc cccatcaagt
     2521 ttataaagtt cagcattatc tggtaaaata gattgtacag actgtgctag tgtttttatt
     2581 atattatctc caaaatccct atcatacatc tcattaacat ttttaaaatc gtcaatatct
     2641 aataatacta tgcataattt ttcaatcata ggatttgaaa ttttatcttc aaatacactc
     2701 ataaattcat gataattcag taactgtgtc aatgaatcaa tcttattttg tttacctaat
     2761 tgcgtcatta ttcctgcaaa aatacttggt tctccaaatt catcacgcat tagttaacct
     2821 ctacatctta accatatata ttctccacta cgatttttag ccctaaattc cactgtatga
     2881 taatccatct ggtttttacc tatttcagtg tttgacttat aaaaacgatt ccagtcttct
     2941 ggatgtacaa tttttttcca gtaaacaagc ggatttttaa caatttctcc tggtaagtca
     3001 aataattcaa cttgtgatgg tgaatatcga aatacgcctg ttttcatatt gcaaatgtat
     3061 atatattcat ctgtacctct aatcaaagca tcataaagca atgtagaatt ataatcaaaa
     3121 ttgtctcttg aaaaattaaa aactcctttt tcttctttat tatttgatat ttcatgaaat
     3181 tcattaatct taattggttt atttaagctt gttgagtttg caatatctat tgattgatgc
     3241 atatgaacaa ctgtgccatc ttgccatgta attaaactat cataattttc aaaaacacga
     3301 tttaacttat tacatttttc ataccacttt attaatactc cttttttatc attctttaat
     3361 aattctaaaa ctttacaaaa actacaagta gaattttttt caggatataa tacttgccaa
     3421 caaacttttc cttctggatc taaaatatta tattcttctt tcattttttt gttcataaaa
     3481 ataatttcat tagtatgaat atttgtaaca tatatattaa tctgtagttg atttaatata
     3541 acttcaaaat tatgtttgtt catatactta tctcctccat agttactgct ctttataaca
     3601 acattgttaa aattattctt tttagaaga
//
LOCUS       DAEMHZ010000230         3450 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000230 DAEMHZ010000000
VERSION     DAEMHZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.233"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            4..1077
                     /locus_tag="KPK72_003723"
     CDS             4..1077
                     /locus_tag="KPK72_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2490888.1"
                     /translation="MNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQYKGKI
                     KSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKK
                     LNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNV
                     LNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQKLNN
                     NFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKP
                     IFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVG
                     KIDDEKNFYPSKESIKVIKSWYE"
     gene            1131..2255
                     /gene="wecB"
                     /locus_tag="KPK72_003724"
     CDS             1131..2255
                     /gene="wecB"
                     /locus_tag="KPK72_003724"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG2490889.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENINSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRVIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2333..3307
                     /locus_tag="KPK72_003725"
     CDS             2333..3307
                     /locus_tag="KPK72_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2490890.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1443 a          332 c          599 g         1076 t
ORIGIN      
        1 tatatgaata aatttaaatt aaaaagaagt attttaatct cattaataat cttaattttt
       61 atatttatag tttactttgt aagtattgag ttaaaagata ggagtctagt aaataatcaa
      121 tataaaggta agattaaatc ggccaattta tctgtagatt atgaaataaa tcaagttatg
      181 aaggatatag ataaattggg attgaataca gtaaatgtac ctataattat aaatgtagag
      241 tccattaatt ctgatattat gtcaatagat aataatagta aagaaaaggc aattaagtta
      301 ataaaaaaat taaataaaaa aggtatttca acaatattag aggcatatcc atggattgaa
      361 aatggaaaat tgtatgagac tgactggaac cctataaata aaaaaagatt tttttatact
      421 tggcaaaatg ccatattaaa tgaacttata attgatgtag caaatccact ggatgtaaat
      481 gttttaaata ttggttctaa ttttgtacat ttggaagaat atcaacaaaa ttggggtgaa
      541 ataatagatt ttgtacaatc caaatttgac ggtcttgtaa cgtacagaac taattggtgg
      601 tatacaaaaa aagatgattt aagtagtaag ttgttttatg aacaaaagtt gaacaataat
      661 ttttttgata agttagattt tatatctata gcagcatatt ttgagttaag taataaacct
      721 gttaatacag tggatgaatt aatttctgcc ttacactcat ctactgtaaa caatagagga
      781 caaaatataa aacaagaaat atataacctt tataaagctc atagaaaacc tatatttttt
      841 ggagaattag gtttttcaaa tagagaaagt gcaagttctc agccgtggaa tcatactcct
      901 tcaaaagctg taaatggcga agaacaagca aggtgttttg aggcatataa gaaagttttt
      961 gaaaatgaag actggataaa tggtttttct gtgttttgtg taggtaaaat agatgacgaa
     1021 aaaaattttt acccatcaaa agagagtata aaagttatta agtcttggta tgaataattt
     1081 tgaaagtgta gaatttatat agattaaata gatgattagg agagatataa atgaatgaca
     1141 tcaaggttat gactgtattt ggaactagac ctgaagctat caaagtagca cctttaataa
     1201 aggaacttga aaaaagagag aatataaata gtattgtttg cgttacagct caacatagag
     1261 aaatgcttga ccaagttata gaaaccttta atataaatgt agactatgat ttggatataa
     1321 tggaaaaagg acaaagcctg aatgatataa catgtaagat tttaaataaa cttcccttga
     1381 ttttaaacaa ggaaaatcca aatataatat tggtacatgg agatacaacc acaactctag
     1441 ctacaagttt gactgcattt tataataaaa ctcttgttgg tcatatagaa gctgggctta
     1501 gaacctatga taaatattct ccttttccag aggagttaaa tagacaactt acaggtatta
     1561 ttgctgatat gcactttgct cctactaatt tggctagaaa aaacttgatt tctgaaggaa
     1621 aacctaataa taatatattt gtaactggta atactgctat tgatgcttta aagatgacaa
     1681 taaaagaaaa ctataatcat cctataattg atgagattgg taacgatagg atgattttgt
     1741 taacctctca cagaagagaa aatttgggta aacctatgaa aaatatattc agagttataa
     1801 agcgaatagt agatgacttt gaagatgtac agatagtata tcccatacat ttaaatccta
     1861 aaatacgaac tatagctgat gagatatttg gaaaatttcc tgaaaaaatt catattatag
     1921 agcctttaga tgtagctgat tttcataact ttttaaataa atcttatatg ataatgactg
     1981 atagtggagg aatacaggaa gaagctccat cattaggtaa gccagttctt gtgttgcgtg
     2041 ataaaacaga aagaacagaa ggaattgaag caaagacact taaattagta ggtactaatg
     2101 aagatagaat atataattca gtgagcgatt tattaataaa taaagataac tatgttcaga
     2161 tgagtaaagc atctaatcca tatggagatg gaaatgcaag caaatatata gtggatataa
     2221 taattaaaaa atttaattgc aaatatttaa attagataag acctaaaaca ttaactaatt
     2281 gatataagat ttatgataat attaaggaat acaattttta agggggagta atatggctag
     2341 aaaattaata aaaaatttgg gtaaaagtaa aagtgtaaag agagtgaaac ttttatttaa
     2401 gaagattttt attactgtat tcatagtagc aagtatagtt gctattttta atataacgaa
     2461 atattttgag gaattataca aagtaaggga tttaaaaagt actaaaattg aatactatat
     2521 ggatgtggct gatgaagcag gagatggaaa ggtccaatta agttggaagg ccttacttgc
     2581 tatagacatg gtaatacatg atgaagattt aagtaatata aaaaagaaag acacattgga
     2641 tataggggaa aagtttatag tagaagataa aaatgataaa ggcgaaaagg tgtataaggt
     2701 aaaaaagttt aataaggtat taagcgaatt gaaatttgac tcttctcaaa aaagtagagc
     2761 aagaaaatac atgaaagatt tagaatacac ataccttgga aataaacaat tagatagtag
     2821 tgatgaaaaa attaaattta taaagaagtt agaagactca gctataagag aatatattga
     2881 ttatggaata ttgccctcta taacaattgg acaagctata ttagaatctg gttggggaaa
     2941 ttctaaactt acaaaacaga gtaataattt atttggtata aaagcagata aagcatggaa
     3001 aggaaaaagt gtagaaattt caacttcaga gcattataat gaaaaaattg tagctagttt
     3061 taggtcgtat aattcattac aagattctgt caaagaccac agtttatttt taattaataa
     3121 taaaagatat agaaaacatg ggttgtttga ggcaaaagat tatattagtc aagctcaatc
     3181 attagaaaat gcaggatata gtacagcaga agataaaaaa ggaaatcgta tatatgcaga
     3241 gctattaata gatgttatta ggagttataa tttgcaattg atagataata aagttgagac
     3301 aaaataattg ttaactttat atgaggagat aacaagataa gatttttttc tacaaaaaga
     3361 atagaaaaat tgtaaaaaaa actaaaaaaa tgtattgact tgttatttaa aaggtgatat
     3421 tattattact gtactcgaaa gagaacaaaa
//
LOCUS       DAEMHZ010000231         3322 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000231 DAEMHZ010000000
VERSION     DAEMHZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.234"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            66..764
                     /locus_tag="KPK72_003726"
     CDS             66..764
                     /locus_tag="KPK72_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490891.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            942..1832
                     /locus_tag="KPK72_003727"
     CDS             942..1832
                     /locus_tag="KPK72_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490892.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2225..3181)
                     /locus_tag="KPK72_003728"
     CDS             complement(2225..3181)
                     /locus_tag="KPK72_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2490893.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         1300 a          381 c          499 g         1142 t
ORIGIN      
        1 gataaaatat aattaagtta agttggtttt tataaacata aatttatttt aaggaggtga
       61 aataaatgaa aatatctagt caatatagaa gtcaatattc atttagatat gaaagtaata
      121 taaataatac aagaataaat gaaagtatgg ttaagaaaaa tgaaactgta ggaaaagaca
      181 cttatttatc taatattatg aaacaaaagc aagaacttaa tgatagaatt agagatttaa
      241 aatatagaca agaggtttat actaaaaaaa taaatgacgc aattaagaac ttatgtaaat
      301 cagaaataag agaaacaact aataactttt ctaatataga aataggtatt aaaaatagca
      361 ttatagaaga gaaaaataaa agtacaatgt tagatgaaaa ttcaacttat ctaaatacaa
      421 atgatgaaaa agaatcttta atcactaaag agtctaatga aaaaattgaa gaagaaatat
      481 taaatgatga aaaattagaa gagttagaac aaaaaaagga ttataaagag gattctaata
      541 aaaaagagaa agtatctgag gacttatctt tagtaggtaa aactcgtgaa gagcttgaaa
      601 atatgcttaa aaattttata aatttaacac aagaagaaat aatgaaactt gagtcgagaa
      661 tagaaaagtt agataaaaat gctgaagaat acaaacaaaa ttcaaagact aatatatttg
      721 ataaaacaga tgaacaaaaa aaacatataa atgtactgat ttaaacaata tgttttaaaa
      781 aaataagttt attatataat gaaaatataa aggtacaatt tcaatacact tctattaaac
      841 caataattta tgaatcagat ttaatttcaa tttactaata accatatatt tatgagttaa
      901 taattttatg ttttaaaaag aattaaataa gggggagtag tatgaagaag aatatattaa
      961 ctactatgat taccatgatt attatattga tgatgagtat gacagtaatg gcagagccat
     1021 atcaagaaga tgaaacatct agcttgaaaa aacagagtgc aaatgaacgt aatcagacta
     1081 atataggcac aatggatggc agagtgcaaa caaatgcaat aagtaaaggt gctaattatg
     1141 accctaaata tcctcttaaa ggaatgttaa cacaacttgg tcttaataca acaggtaaag
     1201 agggatggga tttactctat ggaggaaatc ccaatggagt tgagcttcga gcagatggta
     1261 aatattatta tataaataag ggtatggaag ggctaggatt ttatgcggca gcattgtcag
     1321 gtggctcata tcagagaata ggctttggaa aacttgaccc tatagaagta atagctgatg
     1381 gaaattggat ttcttacaaa caagctcaag atacacttac agtaataagg aattttttaa
     1441 atagttttga ctggagaaat gcatctgaga tggaaagagc taatagagct gcaaaattgg
     1501 taaccgaagc aaaatatgca gactctaaat attgtaacat agtctatggt aatctggttg
     1561 ataaacgtgg tgtatgtgga agttttgcaa gtagctttca tttattaaca aggttaatgg
     1621 gaatggattc actatctata ttaaatccat cattaaatca tgcttggaac tatatacaaa
     1681 ttgatgggaa atggtatagg tttgatgggt ctgaaatatc agcttttggt ggatcattag
     1741 actttgatta ccgtaaatta aaggatgcaa caagggagat gactacttac tatgatgcaa
     1801 aggcattgtc tatattagga tttaatcaat aaaaataggg ggaggcttct tatagagcct
     1861 ttttttattg atttgaaatt taataaggaa ttttatatga aaatttaatc aataaaagca
     1921 aaaaaggctc tttataaagc ctttctaagt gtttttttaa gtaaaatttt ttatatcaac
     1981 atcagtttta atcaaaacat ataacatttt ggacttactt ttaaaaataa aattattaaa
     2041 atataatatt actcatgttt ttagtttatt atattctata atagtggtca tgttgactat
     2101 tgtagtttta ttaagaatag ttggtttata taaatatatg caaagatagc ttgttgttta
     2161 ctttacttat tagtatatag gacaagatac tctaataaat ttgcctattt taatatcttt
     2221 tgtactaaaa ccaactaaag tttcctttat tatttcgttt tgaataatac aaagcataat
     2281 ccgattgttt gtataaagac tggaaagtat cagagttatt ttctaattta gatataccta
     2341 tagaacaaga attactctct aggcataatt ctaaaacctt ctttgcctta ttttcaacat
     2401 tctcttttga ttgattacta tattcaaaaa cagcaaattc atctccacca agtcttccaa
     2461 taatagcatc atttacgaat actttagtca aaatatttga aaaatgtatt agtatttcat
     2521 ctcctcgaac atgaccaaag atatcattta cctgtttaaa attatctaaa tcaaagatgt
     2581 aaaagtatcc atgtgaaaca gttttaagca tttcttttat gtgtacttca aaaaaatgtc
     2641 tattgtatag tccagtcata aggtcttttt ctgattttag tttaaattca tttgccattg
     2701 ctagtgcttc ttgtgctttt tcagttattg ttttagggta atactcatct ttcatttggt
     2761 caaagtttgg aatagaatgg tgaacatgga taagtttaat catatcatct gtaactctgt
     2821 ataccatagt aaatcgagta tccatttcta catagatatc tttttctagg taatcttttt
     2881 ctattaccca aagagtacca tatactataa aagcttcgtc tgtgactttg gtacatgaat
     2941 accactcatc tataatctca aagtctattt gctctgattc tgtttggtct ttttcaagac
     3001 ctataataaa ttcttctaat gtttcgtaaa attcatgttt tcctgttcca attaatgcaa
     3061 ggttgtcatc taataaggaa ataataaatt taaggttttc ttcttctgga tttaaaatat
     3121 aaatatgcca aatttttttt gataaatctc ttaattgttt ttgtattgta ttattttcca
     3181 tagattaccc ccttaagatt tacagtttat attttaatgt attataagtt gaactcttat
     3241 tacaatatta ttataatata gaactaaaga aattacatcc tgtataggta aaagtatatc
     3301 ttttacaata aaaatattac ag
//
LOCUS       DAEMHZ010000232         3285 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000232 DAEMHZ010000000
VERSION     DAEMHZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.235"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..298)
                     /locus_tag="KPK72_003729"
     CDS             complement(<1..298)
                     /locus_tag="KPK72_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490894.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVN"
     gene            complement(315..719)
                     /locus_tag="KPK72_003730"
     CDS             complement(315..719)
                     /locus_tag="KPK72_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG2490895.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            1086..1460
                     /locus_tag="KPK72_003731"
     CDS             1086..1460
                     /locus_tag="KPK72_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2490896.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            1457..2320
                     /locus_tag="KPK72_003732"
     CDS             1457..2320
                     /locus_tag="KPK72_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2490897.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            2320..2958
                     /locus_tag="KPK72_003733"
     CDS             2320..2958
                     /locus_tag="KPK72_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2490898.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         1212 a          382 c          406 g         1285 t
ORIGIN      
        1 aatttacaaa attgtaaatt tgattattgt tatacagtga tatttgttcg tatatatatt
       61 ccctaaataa aggatgaatg ttttgcttaa gttttaaagt aacttcagct ggtatttcaa
      121 aataaagctt ccagtactta gagttatttt tttctaaaat aaaagctact ttgttcaaac
      181 tggtagaact aatctccttt agtgtacaat taaaattata gtgtctcaaa ctagtttttt
      241 cattctcaaa tacatagtca aaccactgta gaacatttct atcaagtttt aaactcatat
      301 aaaaaccttc ttccttattt actaacctta ttaggtcttt ttatctgatt atattctgct
      361 aataaacttt ctttatcagc tctactacct ttatatgcta tatgtggatt tatcatatac
      421 tcaccatttt tcactctttg taaaaaatcc atttttttca gctccgtaaa caatgaagat
      481 aaagaagttt ttggatattc aaattcacaa gataattcat ataatgtttt tctaatctta
      541 ttatttttat ccatagagtt tattaatttt aatggaatta aatccctctt agtatacatt
      601 ccttctacta ctttaatata attcttaaaa caaacattca tatacttact tttattcata
      661 actaaacttt tgctctctgt atattcctga tttaaatcct tttttaatgt cttatccaaa
      721 ttaaatatct cttctcactc tccttatctc taattgataa tataaatata ttacaaaaaa
      781 atatgtatgt caaaactgaa aatgcaaaaa gttcacctta taagcaaact tttatttagc
      841 tataagctaa actttattat ttttttaatt tagtatttcc aatttattgg caggattttt
      901 gaacaatatt tataaatttt ttaaaaaaat ttaaatctat ttaatatggc agactttcat
      961 aaaatacatt acatttatta aatgtataat attggcaatt agttttttct aaaatcagtt
     1021 gacatattat atatatattt atataatgtg tatatactat atatacaatt taaggaggtg
     1081 tcttattgaa tatagttatt agtaattcga gtgggaagcc aatatatgaa cagattacta
     1141 cacaaattaa aagtatgatt ataagtggag agttaccaga aggctcagct cttccaagta
     1201 tgagactatt ggcaaaagaa cttagaataa gtgtaattac tacaaaacga gcatatagcg
     1261 atttagaacg agacggattt attgagacag ttactggtaa aggcagtttt gtatctagta
     1321 aaaacatgga ttttgtcaaa gaagaacaat ttcgtttaat tgaagaatac ctacaaaaag
     1381 cagttgatac agcaaaaagt agtgatatta catatgacga attaaaagat attttatttt
     1441 tattatataa aggagaatga taagtatgga tgcaataact ataaaaaatt taaataaaac
     1501 ttataaagat tttagcctac aagatatatc tttttcagta ccaaaaggga gtgtaatggg
     1561 atttgtaggt gaaaatggtg caggaaaaac aactacatta aaagcaattt taaatttaat
     1621 atcttatgat agtggaaata tagaaatctt tggtcttgat agtaaaaaaa atgaaaaaga
     1681 aattaaagag caaattgggg ttgtatttga gggaagcaac tttcatgaaa atttaaacac
     1741 tgaccatgtt tcaaaaatca tgagtaaaat ctataaaaat tggaatgaca ccttatttaa
     1801 agactactta aaaaaattga gagtaccaga caataaatta attaaggagt tttcaaaggg
     1861 gaataagatg aaacttagta ttgctgtagc cttatctcat aaaccaaaat tattaatact
     1921 agatgaggca actagttcac ttgaccctat cgttcgtgaa gaaatactag atatattttt
     1981 agattttata caagatgaag aacattctat tatcttatct tcacatatta caagtgattt
     2041 agataagata gcagactata ttacatttat acataaagga aaaattgtat ttagtgaaaa
     2101 taaggatgaa ttaattgata ctatgggagt tttaaaatgt aaacctagtg attttgataa
     2161 cctttcaaga gaggattatt catattatcg taaaagccaa tttggatatg aagtacttct
     2221 taaagataaa catagattta tctcaagaca tccaaactgt attgttgata atacgtctat
     2281 agaagaaatt atgttatttt atgtgagagg tgataaataa tgaaaggtct aattttaaaa
     2341 gatttactta atttgaaagg aaatataaag tttatacttt tattcatcat tatgtttggc
     2401 tttatgtctt ctttaggaga tggaaatgtt aataacttta taggtgttat tatagtttta
     2461 tgtactacaa tgattgtttc aacattttca tatgatgatt tgaataaatg ggatagttat
     2521 gtacttacaa tgcctattaa tcgcaatgat attgtattaa gtaaatattt aactatgtta
     2581 atatttagct ttataggtgt tcttgtttca cttattgtta gtgtaactat tggttatttt
     2641 aaaaatacac ttatattaaa tgaaacacta cttattaatg cactaatctt atcaatttca
     2701 gtatgttttg gtagccttat tctaccactt atttataaat ttggtacaga aagagctcgt
     2761 ttattaatga ttttatgctt tttagttcca acactagctt tattagtttt taaaagtatt
     2821 ttagaaaata ttagttcacc tatttctata gaaattatac ttaatacact agtatattct
     2881 cttccatttg ttgctatttt actatttgtg atttcatatt ttatatcatc aaaaatctat
     2941 agtaaaaaag aagtataaaa tatactatat atatttctgt atttaagtat ttaaataaaa
     3001 tactatcgag tttattttgg tagtatttta tttttcaact attaatctct actaggtatt
     3061 tattatttta ttacttatat ttaataagac tatttactac tttctaatct tatttatata
     3121 atttattcat tttataaatt gaactactaa tgatattatt gaacatagca atagaataac
     3181 atctaaaata gtttttttgt attaaattaa cttattttaa tattcaacaa tttaggttta
     3241 ctaaatttat tgatgatttc tttttatata acttaaacta cattt
//
LOCUS       DAEMHZ010000233         3218 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000233 DAEMHZ010000000
VERSION     DAEMHZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.236"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..729
                     /gene="cdtR"
                     /locus_tag="KPK72_003734"
     CDS             <1..729
                     /gene="cdtR"
                     /locus_tag="KPK72_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG2490899.1"
                     /translation="DNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLLKELTEK
                     NKVRIYFIVVDAKYKISNELCDGLWIAQKIRELDYISPIIFLTNHIEMILGIFDYRLE
                     VMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISFDEVIYF
                     ETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLDLKKNNI
                     KMSNGHICRISNTYRNILKNIIKT"
     gene            1118..1300
                     /locus_tag="KPK72_003735"
     CDS             1118..1300
                     /locus_tag="KPK72_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490900.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            1518..1661
                     /locus_tag="KPK72_003736"
     CDS             1518..1661
                     /locus_tag="KPK72_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490901.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            1636..3202
                     /locus_tag="KPK72_003737"
                     /pseudo
     CDS             1636..3202
                     /locus_tag="KPK72_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
BASE COUNT         1400 a          350 c          430 g         1038 t
ORIGIN      
        1 gataatgatg tttgttttgg aatgaaacta aaagaaaaaa taaataatat tttaataaaa
       61 gaaggttttg ataatgatgt tgtaagatta tatcataatg caaatttgtt attaaaagag
      121 cttactgaaa aaaataaagt aagaatatat tttatagttg tagacgcaaa atataagata
      181 agtaatgaat tatgtgatgg attatggata gcacaaaaaa ttagagaatt agattatatt
      241 agtccaatca tatttcttac aaatcatata gaaatgattt tagggatctt cgattatagg
      301 ttagaagtta tggactttat actaaaacat gatatggaaa ttgctgaaag taaaataaaa
      361 gcttgtatta aaattgctca caaaagatac attaaagaaa aaaattatcg ctctaatttt
      421 tttactatct attcagattt ctcactatgg aaaatttcat ttgatgaagt tatatatttt
      481 gaaacaagca ctattccaca taaaataaaa ttagtgacta cttctagaat atttgaattc
      541 tataaaagtt taaagtcttt accagattta gatgcatgct ttatccgtgt acataaatct
      601 tttgtagtta ataaatatca tattgtttct ctcgacctaa agaaaaataa tattaaaatg
      661 agtaatggcc atatatgtcg tatatctaat acatatagaa atattttaaa gaatattatt
      721 aaaacataat aaatagaaaa aggagatgtc tcaagataaa aatttgaacc atctcttttt
      781 tatacaaaaa gagtaatccc taagaatcct ctatataata atcatattgt gaaaaaacac
      841 atactgacta tatcatatat aactactatt agatattata acaattgttc ctagattatg
      901 attcgataag atagctcatt aaatcataaa agtacgaaat agtctcttta aaatattcag
      961 ttgttatttt gtactgacat atcatataaa tacatatttt tatgatatat acctacatat
     1021 tttatgaaat ttatataaaa aattcttatt tagattatat aatctaaata aattaaaatt
     1081 caaaagttaa ctaaactaat atttgtaggg agaataaatg aaaaaattta ggaaacataa
     1141 aagtattagt aattgtatat ctatattgtt gatattatat ctaactttag gtagtttgtt
     1201 acctaataac atttatgcac aagatttaca aagctatagt ggaaaagttt gtaatactac
     1261 ttacaaggct cctatagaaa gatcagaagg atttttttaa agataaagaa aaggctaaag
     1321 aatgagaaag aaaagatcaa aaacttgaaa aatctgaaaa agaagtatta ggatcatata
     1381 aaaaagattc tatagagata agcaaatatt ctcagacaag aaattatttt tatgattatc
     1441 aaatagaagc aaattcttga gaaaaagaat atagagaact tcgaaatgct atatcaaaaa
     1501 ataaaataga taaacctatg tatgtatatt attttgaatc tccagaaaaa tttgcattta
     1561 ataaagtaat aagaacaaaa aatcaaaacg aaatttcatt agaaaaattt aatgagttta
     1621 aagaaactat acaagacaaa ttattaccat acaacagata attcaactgc tgttcaaaat
     1681 agcaatggat aatcatgaaa tactggatta agtataaaca aaggagaatc tgcatatata
     1741 aatgcaaatg ttagatacta caacacaggt actgcaccta tgtacaaagt gacaccaaca
     1801 acaaatttag tgttagatgg agatgcatta tcaactatca aagcacaaga aaatcaaatt
     1861 ggcaataatc tatctcctgg aaatacttat ctcaaaaaag gattttcacc tatggctctt
     1921 aacacaatgt atcaatttag ttctagactg attcctataa attataatta attaaaaaaa
     1981 ttagatgatg gaaagcaaat taaattagaa acaacacaag taagtggaaa ttttggtaca
     2041 aaaaatggtt ctggacaaat agtaacagaa tgaaatagtt ggtaagacga tataggtcaa
     2101 attgacagta tttctgtatc tattatatta gatacagaaa atgaatctta caaaagaaga
     2161 attactgcta aaagtttaca gatcccagaa gataaaacac ctgaacttac aattggagaa
     2221 gcaattgaaa aaacttttgg cactactaaa aaagatggtt tgttatattt taataatata
     2281 ccaatagatg aaagttgtgt tgaactcata tttgatgata atatagcaat aagattaaag
     2341 acagtttaaa aactttgtct gataaacaaa tatgtaatgt taaacttgaa agaggaatga
     2401 atatacttat aaaaacacca acttacttta ctaattttga tgattacaat aattacccta
     2461 gtatatggag taatgttaat actacaaatc aagatggttt acaaggctca gcaaatagat
     2521 taaatggtga gacgaagatt aaaataccta tgtctgaact aaaaccttat aaacgttatg
     2581 tttttagtaa aattcaaaaa atcctttaac atataattca ataattgtaa atataaaagt
     2641 aaaagaagaa aaaacggatt atttggtact agaacaagga tatacaaaat ttagttatga
     2701 atttgaaact actgaaaaag attcttctaa tatagatata acattaattg atagtgatac
     2761 aatatactta gataacttat ctattacaga gctaaatagt actcctgaaa tacttaatga
     2821 accagaagtt aaaattccaa ctgttcaaga aataatagtt acacataaaa tatattctgc
     2881 agattttaaa tttaatccaa gtacaataaa tgcttatata aatggtatgt attttacacc
     2941 aacataaact aataaagaag ctctcgatta tgtccaaaaa tatagagttg aagctacttt
     3001 acaatatttt ggatttaaag atattgtaac taaagataaa gaattgcgta attatttagg
     3061 agatacaaat caacctaaaa ctgattgtgt taatcttagg agttatttta caggtggaga
     3121 aaatattatg acatacaaga aattaagaat atatacaatt actcaagatg atagagagtt
     3181 attggttctt agtgttgatt agtatcaaat gatttaaa
//
LOCUS       DAEMHZ010000234         3200 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000234 DAEMHZ010000000
VERSION     DAEMHZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.237"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            355..2997
                     /gene="adhE"
                     /locus_tag="KPK72_003738"
                     /gene_synonym="adhC"
     CDS             355..2997
                     /gene="adhE"
                     /locus_tag="KPK72_003738"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2490902.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1232 a          404 c          619 g          945 t
ORIGIN      
        1 ctacttatac aaataacatg caaataacat ataaaattta ataagtaaaa aaaatgaaag
       61 atataaatta tcattttagt attttattta gtcgaagtaa attattcaaa tgaaatatat
      121 ctcatttaga taaaaaattt tagtaaaagt aaaaaaaata atatatttgt taaaaaataa
      181 attgacaaaa tattcagaaa aaaacttctt gacctaagag agtaaaataa ataaaatcta
      241 aggtatagag atgaaatcta taaaattatt ttaagtataa gttaaaaata tagatgatgc
      301 atttaagatt tataaaaatt agagatgcat aattatttta gggaggtttt tagtatggag
      361 aaaaaagaaa aggtagtaga aaaatcaaat gttgaagtat gtagtcctga aattgtcaac
      421 agtgttgaaa cattaagaat gagattagaa gaaattagat tggcacaaaa agaatttgca
      481 acatttacgc aagaacaggt agataagatt ttcttagctg cttcaactgc agctaatcaa
      541 caaagaatac ctctagcaaa aatggcagtg gaagagactg gtatgggtat tgttgaagat
      601 aaggttataa aaaaccattt tgcctcagaa tatatctata atgcttataa agatactaaa
      661 acttgtggtg tcatagaaaa agatgaagca tttggtttta caagaattgc tgagccagta
      721 ggtgttttat cagcagttat accaacaact aatcctacat caactgcaat attcaaatcc
      781 ctaatagcac taaaaactag aaatggtata attttctctc ctcatccaag agctaaaaac
      841 tgtactatag aagcagctag agttgtacat gatgcagcag ttaaggcagg agcaccaaaa
      901 ggacttatag gttgggtaga tgttccttcg attgaattaa ctaatgttgt catggcagag
      961 gcagacctta tattagctac tggtggacct ggaatggtta aatctgcata ctcttcaggt
     1021 aaaccagctg tcggagttgg gcctggaaat gtacctgcaa taatagatga gagtgcagat
     1081 ataaaaatgg ctgtaagttc aatcttagta tcaaaatcat tcgataatgg tatgatatgt
     1141 gcttccgagc aagctgtaat agtaccagaa aaaatatatg aagaagttaa aaaagaattt
     1201 aaatatcgtg gagcacattt cctaaataaa gaagaaactg aaaaagtagg aaaagtagta
     1261 ataattgatg gttcattaaa tgccagaata gttggacaac cagcacatgt aatagccaaa
     1321 atggcggatg tagaagttcc aaaaacagct agaataataa ttggtgaagt agaatctgtt
     1381 gaattaaatg aaccttttgc acatgaaaaa ctttctcctg tacttgcaat gtacaagtct
     1441 aaaagttttg aagatgcagt tgctaaagca gaaaaattag ttgcagatgg aggctatggt
     1501 catacatctt cattatatgc agactctata aatcatccag agagagtaga aaagtttgta
     1561 aatgctatga agacttgtag agtattagtt aatactccat catcacaagg tggtataggt
     1621 gatttatata actttaaatt ggcaccgtct cttactcttg gttgtggttc ttggggtgga
     1681 aactcagtat ctgaaaacgt tggagtaaaa catttgctta atatcaagac agtagctgaa
     1741 aggagagaga atatgctttg gttcagagca ccagaaaagg tttatttcaa aaaaggatgt
     1801 ttaggtgtag cagcaagaga atttaaggat gttatggata aaaagaaagc atttatagta
     1861 acagattctt tcctatataa taatggatat actaagaaat taactgattt attagatgag
     1921 atgggaatta agcatactac attctttgat gtagctccag acccaacatt agcttgtgca
     1981 agagaaggag ctaaagctat ggctgatttc caaccagatt taataatagc tgtaggtgga
     2041 ggaagtgcaa tggatgctgg taagataatg tgggtaatgt atgagcatcc agaagtagat
     2101 ttccaagact tagctatgag atttatggat ataagaaaaa gagtatatgt atttcctaag
     2161 atgggtgaaa aagcgtattt tgctgctatc ccaacttcag caggaactgg ttcagaagta
     2221 actccatttg cagttataac tgaccaagat tcaggtgtaa agtacccatt agcagattat
     2281 gaattaatgc caaacatggc tataattgat gctgatatga tgatggagat gccaccaaga
     2341 ttaacagcag catcaggtgt tgatgcttta acacatgcat tagaagcata tgtatctatg
     2401 ttaagaactg aaccagctga cggtctagca ctacaagcag gtaaaataat atttgaatat
     2461 ttacctcgtg catataaaaa tggaaagaat gataaagaag caagagaaaa aatggcaatg
     2521 gcatctacta tggcaggaat gtcttttgca aatgccttct taggaatatg tcactcttta
     2581 gcacataaat taggtgcatt ccaccatgtt caacacggtg ttgcaaatgc attattaata
     2641 aatgaagtaa tcaagttcaa ttgtgcagaa gctccaaata agatgggtgc attctcacaa
     2701 tacagatatc ctgattgcat acaaagatat gctgaatttg cttcttttgc aggtattaaa
     2761 ggaagtacag accaagaaaa agtagataat cttataaaag caatagatga actaaaggca
     2821 aaagtaggtc ttcctaagac tataaaagag gctggagtag aagaatctaa attcttagaa
     2881 agattggatg ctatggttga acaagctttt gatgaccaat gtacaggtgc taatcctaga
     2941 tacccattga tgagtgaatt aaaagagata tatctaaaag tttattatgg taaataatat
     3001 ctattgtgca acataggttc tattgggttt gtatataaat tattagattt gaatataaat
     3061 agagttttat tagagatatg tataaatagt tataaataat agattataac ttaaattctg
     3121 aatggaaatg ataaaaggtc agtaatttaa agataatttt gaatatgtgt atttaatcaa
     3181 ttaactttaa aattagtcgg
//
LOCUS       DAEMHZ010000235         3139 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000235 DAEMHZ010000000
VERSION     DAEMHZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.239"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..670)
                     /locus_tag="KPK72_003739"
     CDS             complement(<1..670)
                     /locus_tag="KPK72_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2490903.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFEL"
     gene            complement(710..1057)
                     /locus_tag="KPK72_003740"
     CDS             complement(710..1057)
                     /locus_tag="KPK72_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490904.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1275..2231)
                     /locus_tag="KPK72_003741"
     CDS             complement(1275..2231)
                     /locus_tag="KPK72_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG2490905.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQETLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2674..3042
                     /locus_tag="KPK72_003742"
     CDS             2674..3042
                     /locus_tag="KPK72_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG2490906.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1133 a          419 c          398 g         1189 t
ORIGIN      
        1 tgagttcaaa attttcatct tcaaagtagg gaatataata taaaggtgtt ttgagagcag
       61 gtaatttatt atataattct tcaagaatat cgatattttt aggggagtta aagaaaaagc
      121 atttacatac gttcattaaa tccacagcat ctgaaagtga gttatgttta atatctcctt
      181 ctacagaaaa aatttttttc atattcagta aagatatttg cttagataag attctattat
      241 tgtatgtaat atgttgagat ataaaaggtt gaatgtcaat catcatatta aataactcta
      301 agcatttacc tgtatattta tatactctaa atgaagttag aaggccagtt atatcaaaat
      361 caccccaatt atatattgta acatcatcaa atttacaaaa ccatttataa aaatcatcca
      421 taacatataa aagattagga gcattattga tatctagttg gttcaatttg gttaaatttt
      481 tacatttatt tgaaagtatt ttattgtatt ttggcttaac taaagagtaa taattatcta
      541 ttgtattaaa ttcgttatca actaaaactc caccaataga aattaattcc gcaccattat
      601 attcttttgg tttattttta ttatcatcta gtatattaaa ttcaaagtct ataaataact
      661 tgtatttcat aaatctaaag cacatcctca ggtaaattat ttattgagcc taagcatcat
      721 aagaatcaat agatataatg actctaaaca tcaaaggttc attacaactt ttatttattt
      781 cataatttaa ttcaaataaa tcttttaaga tataatttat aagtattctt tcccaaccca
      841 tttttaaatt aactttagag tctattaata tattttctat ctttttaaat atttctatat
      901 ttgctggtat tatactgagc tcaatattac ttttaaacgg gttagactgt aaatctgtta
      961 taagtatatt ttttaattcc tctgtaagtt cagaaattaa actatcacct atgtattttt
     1021 ttatgatttc ctctctaatt tcttttaaat ttagcaatat tagtacctgc tacttacata
     1081 gtagcttttc ctttcctttt aatactatta ttcatttttg aatcttattt agatatattt
     1141 taagattaaa agtctttagc tatataattt tttacccatt tttaacttat attaacatat
     1201 tacaggctta tatctccacc ctgcttacat tgaggatgga gtatattgcc tattttgata
     1261 aaaatgatag gttgttattt gcctattcct ttttctgata atttttctaa catatctttg
     1321 gtcattttat ctaaatcata actaaactta aagttccatt cttttactgc actactactg
     1381 tctagagagt taggccaaga atcagctata ctttgtctta cagggtctac atcatattct
     1441 atcacaaaat caggaatata tttttttata gaatcagcta tttcttcagg agaaaagctc
     1501 atagcagtaa tattaaaagc atttctatgg atgagtttat ccgctggagc ttggattaaa
     1561 tccactattg attgaagtgc atcaggcata tacatcatat ccatttttgt accttctgct
     1621 atgtaagatt tatatctctt attttttaat gcctcataat atatatcaac tgcataatct
     1681 gtagttcctc caccaggagg cgtaacataa gaaataagac ctggaaatct aacacctcta
     1741 gtgtctactc caaattttga gtgataataa tcacatagta attctccaga aactttagtc
     1801 acaccataca tagtattagg tctttgtaat gtctcttgag gagtcatatt ttttggagaa
     1861 ttttcaccaa aagcagcaat tgaactaggt gtaaaaaatt tacaatccaa ttctttggct
     1921 atttcaagac catttattaa tccattcata tttaagttcc aagcttctag aggtttactt
     1981 tcagcaactg cagaaagtaa agatgctaga tgaattatag tatctacatc atattttttt
     2041 gcaatttcaa aaaaacgatt cttatctgta acatctaata tctcaaatat accagattca
     2101 catactggat taccttcttt tactctcctt gaactagcga ttacattttg ttctccatac
     2161 tcatttctaa gctttattgt taattctgaa cctatttgtc ctaatgcacc tgttataagt
     2221 atttttttca attttacaac ctcctaaggg tttaatttat gtagtactac ttatttttat
     2281 tttagcaaat cttttgttta aatacaaaga attaaagata ataatatacg acttaattga
     2341 atgaagttca gtaagtgcct aaaaatttaa agctaatata taatggtata ttgtataaca
     2401 ataaaagagt agattaaatt attatatata gattgtgtga ctaagtcaca gaaaatagag
     2461 ttaatttaat atataataaa agcatatcaa gtaaagcttt aaattaaagt aactttatat
     2521 aaaaagtaat ataaattgat aaaattataa atttatttat agtgaatata ggttggattt
     2581 tatagataca aaaatagtat atatgatatg tagaccatta gcatgtttaa agtagcaaaa
     2641 catataaaat aaaatttgaa aggaagtata aaaatgacaa gaccatcaat atatcatagc
     2701 caaggataca cttgtgcaga ggcattaata aagtcttata atgaggaaca caacacagat
     2761 ataccaatct caataggaag tggtatgggg gttggaatga atgttggaag tgtttgtgga
     2821 gctgtaaatg ctgctgttgt tataataggg tatttaaatg ggcgaaatag taatttagat
     2881 gaaaatatag caaggaagta ttcaagagag ctattgaata aagtaagaaa taaatacagc
     2941 tcagaaatat gtgtatctct taaaaaaaat aaagtaactt gtgaagaaat aataaatttc
     3001 acatatgatg ctctaaatga tatgctaaaa aatcaaaaat aattctaaga aacaagtatt
     3061 ttacattcga tagaatactt gtttttttat gatattttta ataggtatta gtttattata
     3121 atttttaaat caattttat
//
LOCUS       DAEMHZ010000236         3017 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000236 DAEMHZ010000000
VERSION     DAEMHZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.240"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..478)
                     /locus_tag="KPK72_003743"
     CDS             complement(<1..478)
                     /locus_tag="KPK72_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2490907.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQ"
     gene            complement(1287..2324)
                     /locus_tag="KPK72_003744"
     CDS             complement(1287..2324)
                     /locus_tag="KPK72_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2490908.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2421..>3017)
                     /locus_tag="KPK72_003745"
     CDS             complement(2421..>3017)
                     /locus_tag="KPK72_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2490909.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT         1026 a          465 c          396 g         1130 t
ORIGIN      
        1 tttgagagtt atcaccaaca ccttttaaag tgtatccaag acctgttttt aggaataaat
       61 ctaaaaggac tttacagatg aatacaaaag ctaaggctaa aactatagga gatatttccc
      121 cattgaataa gtgtttaaag ctaaatagag gaatatttga ttttcccatt attcttaaat
      181 tgatagagta tagcattccc ataactaaaa taccagaaag taagttagat atcttaaact
      241 taatgtgaag aatccctgta acaagaccag cagtacaacc acataagata gccattaaag
      301 ttgctactac aggagaaata ccattagtaa gagaaaatgc tacaatagaa gcacccattg
      361 tgtagctacc atcagcagac atatctggaa aatctaagat tttgtaagtt atataaaccc
      421 ccagtgccat aatcgataaa attaaacttt gcgtcattac agatataata cctgacataa
      481 ataaaacact cctttgaaaa tatttttata attttaatta tttgctaatt ttgtgtataa
      541 aaaaaccaca tagtgtacaa aactatgtgg taaaaacacg aataagccac atagtttaat
      601 ctatgtggct cttatatagt taaatgaaaa ctttataaac aatatagcca acatagatac
      661 aatcccaaaa ttttagtagt cttaaggggt ctgtatctat gttaaaacgt aggtacaaac
      721 cctaaaaata gtggctataa taataataat attttctatt gtttaataaa gtattcataa
      781 ttatctctcc cttttaatat tttaattagt cagaaaatta tatgtattct cgacttgtta
      841 taaattataa attatacaat aataaaagtc aactgctaaa tttaattaaa atactaaata
      901 atgtatactc agaatacttt taaattaatg tattgtttgc aaaaaacgtt atcctaagtt
      961 ataagtgaat gtggaaattt tatcaaaata tttactaaat atggaataac tagacatgta
     1021 tatcaatcaa atatcataaa aaaaagctgt ctggagaata ttaaaattct tttggacagc
     1081 tcaagtagtg attagaaagt tatataactt tcataatcag tcgtcaatat agacgacttt
     1141 tatggaaaat gaataacttt atatgcaata ttcaaattgc tacaataagc acaattttca
     1201 aagggttagg gtggataaga gtacttatta tataaagttt tcatattacc tacttcatat
     1261 tacctatttt ttgaaattaa taagttttat tttgcagatt ttgcattttc atctgacttt
     1321 atatacttta tattatctgc tgataacttt tgcatgtcaa gtgcttttag tgtatcttcg
     1381 ttaattatta tttcagtttc atctaatgta gtaactggta tatcgccaac agattttcct
     1441 tctaatactt caatagcaag ttctccagcc ttataaccta gtttttcata atctattcca
     1501 caacaagcta aagcaccaga agacacagaa ccttcttcag aggctattat aggaatcttg
     1561 ttttcattag caactttaga aactattggc atagaagaaa ctattaaatt gtcagtaggg
     1621 acatataaaa catctatttt gccaactaaa ctagaaatag cttggttaac ttcacttgaa
     1681 gagctgattc ctttttcaac tacttcataa ttatttttct tagcataatc atgtagagaa
     1741 tcaacttgga tttttgaatt aacttcacta gtattgtata taacccctat tttctttgct
     1801 tttggagtca atgttttaac caattccaat gttttatcaa ttgaaagata atcagatgta
     1861 ccagaaacat ttccacctgg tttttcaaga gatttaacaa gtccagcttc tacagggtct
     1921 gtaacagcag tcattattat cggaatatct ttagtagcat tataagctgc ttgtgcagat
     1981 ggagtagata tagcatatat caaatctttt ttgtcggata caaacttact agcaatactt
     2041 tgtgtagtag gcatatcatt ttgtgcattt tggaaatcta tttttatatt atctccatct
     2101 ttatagcctt tatcttcgag tgctttgatg aatcctttct ttgctttatc cagagatgga
     2161 tgttccacta attgactaat acctatattt ttttgttttt tgctatctgt ttctttattt
     2221 tcatttgaag cattggagcc atcattttga gaacatccag taagcattga tacaccaagt
     2281 actcctgcta taattaaact taatattttt ttacttttta tcattttatt ttcctccctt
     2341 aaaatttatt gattatattt gttcatatat tcatatgata gattataaat atttctgatt
     2401 aaaactaaga ttttaatttc ttatactgca tattcaaatt tttctaagat ttcttcaact
     2461 gttaaatctt ctttttcttt atcttttaca tctaatacaa cttcaccctt atgaagcatt
     2521 atcaacctat ctccatattg aagagcgtgt ttcaagttgt gagtaaccat aagtgtagtt
     2581 atatttttct ctcttacgat tttatctgtt aactctatta cttcatttga tgtttttggg
     2641 tcaagtgctg ctgtatgttc gtctagtaat aagaccttag gacttgctaa gctagacata
     2701 attagtgata gtgattgtct ttgaccccct gaaagatatt gtactttaat atctaaatat
     2761 ttttttaaat ccaaagaaat tccttcaagt aagtgttcca agtcattcgt tttatgacga
     2821 agacattttt ttaagttcaa taatttccct ttattaagtg ctagagataa attttctcta
     2881 accgtcatag aaggacaagt tccaaggctt gggtcttgaa aaactctact tactatttga
     2941 gttcttttat actcagctaa atttgttata gtagtactat ctaaagtaat ttgaccagaa
     3001 gattctttta taagtcc
//
LOCUS       DAEMHZ010000237         2978 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000237 DAEMHZ010000000
VERSION     DAEMHZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.241"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            172..1563
                     /locus_tag="KPK72_003746"
     CDS             172..1563
                     /locus_tag="KPK72_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2490910.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            1670..2710
                     /locus_tag="KPK72_003747"
     CDS             1670..2710
                     /locus_tag="KPK72_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2490911.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
BASE COUNT         1182 a          311 c          520 g          965 t
ORIGIN      
        1 atttttactt attaaaaaaa atttatacta gaaaagtttg gttttttgat taaagtggta
       61 tataatagat atataaatta atatttaaaa agtagttaat attaggggag ggcatttgca
      121 cctcccttgt aatatgtgga aaattgaata ttagaagaag gaggagactt gatggaaaat
      181 caacaattat taggaacaga acgaattagt aaattactac taaaatattc tataccagct
      241 ataataggta tgttggtaaa tagtttatat aatgtagtag atagaatttt tattggaaat
      301 ataccagggg ttggaccact tgcaataaca ggtcttggtg taacaatgcc aataatgaca
      361 ataatacttg catttgggat gttgataggt ataggaacta ctactaccat atcaataaaa
      421 cttgggcaag gtaaagtaga agaggcaaga aaacttatag gaaatgcaat gacgttatct
      481 gtaataacag gaattatcat aatgatactt ggaatattat ttgcaaataa aatacttaca
      541 ttgtttggag caagtgaaaa tactttaata tatgcaaaat catatataaa tattatatta
      601 ttaggtacag tagtaaatct tctttccttt tctttaaacc actcaataag agctgatggt
      661 agtcctaaaa tttcagcagg aataatgata gtaggatgtt taactaatat agttttagac
      721 tggatattaa tatttggatt caatttggga atacaaggag ctgcaattgc tacagtaact
      781 tctcaggctc ttacagctat tttaactata gggtattata taagtggaaa atcaaactta
      841 cgattcagta aatctaatct aaaattagat aaaaagctaa taaaagcagt gtttgcaata
      901 ggtatgtcgc catttgcgat gcaacttgca gcaagtttag ttcaagtaat atcaaatatt
      961 gcacttaaaa ctcatggtgg agatttagct ataggggcta tggctactat ttcttccatt
     1021 gcaatggtat ttttgatgcc tatctttgga ataaatcaag gagcgcaacc tattataggt
     1081 ttcaattatg gagctgaaaa atatgatagg gttaaaaaag catatttagg ttcattagta
     1141 gtagctacta taattttatg tatgggtatg gtagtagtta tgcttttccc agaagctatt
     1201 ataggtatat ttaataaaga ccctgaactt atgaatatat ctgttaatgg attaagaata
     1261 tacttactta tgttgccaat tgtaggctta tcagttacag gaacaaactt tatacaatct
     1321 ataggaaaag caaaaatggc tatgttattg agccttttaa gacaagtaat tttattgata
     1381 ccagcagtat taattcttcc tacattctta ggtttacagg gtgtatggac agctcaacca
     1441 gtgtctgatt ttatagctac agtaattaca ggtatagttg tatttagaga attaaaaaga
     1501 tatactccta aaactgaaaa attaaatgaa aatgaaagac taaatgagat aactactgag
     1561 taaaaatata aattcagata attgagcaag aaattgcaaa aaatacgatt atatgataaa
     1621 ataaaaccgc tgtcaaggat aagagaaatt ctaattatgg agtgatttta tggaatttac
     1681 aaagaaaagc aaattaaaaa atatgtatgt aaacatgaat gtagctagaa tgttgtctaa
     1741 gataaatgag tataagggta ggcaactgct ttacaaaaaa caaccaaaag aaattttaga
     1801 aaacctagaa aaaaaatctt tagtagattg ttctgaatct acatacattg gaaatcaaag
     1861 agatagtagt aattttaatc tagaaaagtt gataagtaat gaagttacac ctagaagtag
     1921 agaggaattg agtattgttg aatacagaga tgtggtaaaa actattaaca gtgcttatga
     1981 gagtattccc atcagttcac aaacgattct tgagcttcat gggtacttat ataaattttc
     2041 atctactaga ggaggaagtt ataagtcaga taatgatttt attgaacaca atttaaaacc
     2101 tagcgaattt ataagtattg attcaagtgt caataaagtt gaaaaagctg tagaagaaat
     2161 atgtgaagct tataatacat tgatagagga agatgaaatt gatatattaa tattaatttc
     2221 tgcatttgtg ttagatttca tattgataca tccattcaaa gaaggtaata taaaaatggc
     2281 gagagtactt atcctattgt tactaaataa aaatgggtat gaagttggaa gatatataag
     2341 tttaggaaag atatttgatg atagttcata tgaatattat agtaatttaa attatttgaa
     2401 agcctcaata ggtagtgaaa aagcagatat gaatgcatgg attgagtatt ttttagagac
     2461 gatattaact gcatatgaga agctggatga tagtttaaat atatctgata agaaaagaca
     2521 aacaaaaact agtagaatag aaaaaataat aaattctacc cttggatatt ttacaaaaga
     2581 agatataaga gatttatgtc ctgacattcc agaacctact ataaatagag tttttaataa
     2641 tctaagaaaa caagataaga tagaagttgt tgcaagagga agaagtgcaa aatggaagaa
     2701 aaaatattaa aaaatgttta aataactaaa atggtatagt gttttacact ataccatttt
     2761 taatgtcatt agtatgagca ataatttaac aacttaagca atatcaattt tatttattga
     2821 ttatttttga actgaaaaat ggagttatat ggtatactac aataataatt aattttggat
     2881 gagaatatat aacgttacag aatatcttag gagtatatgt ataaaaaata taataaaagt
     2941 gtgaggttta gtaattaaca aattatgtgg cttcacac
//
LOCUS       DAEMHZ010000238         2907 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000238 DAEMHZ010000000
VERSION     DAEMHZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.242"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(675..1304)
                     /locus_tag="KPK72_003748"
     CDS             complement(675..1304)
                     /locus_tag="KPK72_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG2490912.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1383..2345)
                     /locus_tag="KPK72_003749"
     CDS             complement(1383..2345)
                     /locus_tag="KPK72_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2490913.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
BASE COUNT          994 a          447 c          294 g         1172 t
ORIGIN      
        1 aattagttta tttcatctat catctcaatc atatcattta ctaactatta ttctaatgag
       61 catataacat tgaaatttat aattatacaa cctaaatgcc tcccaatgta ccctcaaaaa
      121 catcatttcc tgtaactgaa gtataattgg tatattttat cactgaaata aaaaaacact
      181 agtcaataaa gactagtatt ttcaagtatt ttatcattct ttttttaatt attggagacg
      241 aatcattaga ataatgccca taatttatct atatgcaaat tctaaattta aaataaagtt
      301 tcagataaat ttactattct atgctatgat acaacagaaa aaactgaaat ctatgagtgg
      361 tagcttcata taatcctagt tcctacagta atcaaaactg aggtttattc tatatgtaac
      421 taatgataaa ttttattata ggagttatta gctctatttg tgttactata agtagtaatt
      481 atatatatga taatttttta acacttttct gttggctata tagaaagtgg tttagaattt
      541 aaatttaaat aaaatttaaa ttcaccaaaa agaaatctat attagaaacc actactctat
      601 tccaccagat tgtaattttt tgttctttat atcacaatac tataccatat taaattgaaa
      661 aataaatttt aaatttattt gttacaattc attaattcaa aaaaatattt tgagtcatga
      721 gttttgtaaa gatttagcca tgaatttaat gcttctgatt caaaaacatc taaaagtttc
      781 aatacttgaa acgtaaattc caacggagca attcctgaag ccgtaactaa attttcatca
      841 ctaactacag attccgtttt ataatatttt tctccagagt aatttggtat taccatttta
      901 agaaactcta agtcattact tgtatgattt ctaacatcta atagcccttt tttagctagg
      961 cctaatgtag ctccacatat ggctgcaact atagaatctt gtattaaagc ttctttaact
     1021 ttttctaata ttatctcatg attcatggat aaccaagtat ttccccctgg caaaattaat
     1081 aaatccttac tttccatatt aaactcatca actgtaattt caggtaaaac ttttaaccca
     1141 cccattgtta taataggatt tttatcaatt cctacagtaa ttacttttag tggttttaat
     1201 ccttttttaa aatatcttcc tgtatttaat tcagcagtta aatatgccac ttcccaatct
     1261 gacatagtat caaatacata catatatact tttctatttt tcatcttttt tactccttat
     1321 gtaattaatt tatatatcta attataaaat aacttaactg acatattcag tcagttaagc
     1381 tattaaattt gataataatc aattaattct agtaaatctt caattatttt cttcctaagg
     1441 cttagtggtt caattacttg tattgtcttt ctataaggta aaagtatatt tggtatgtac
     1501 ttgcatatta cttcctcttc aagcataaat attattttat taggtgttct ttctttcaag
     1561 tagtgcccta aaaaccaatg ttgacttaaa ctatctaaag aatctatact cccttctaca
     1621 actaattgaa ttatattttg ttcatctttt atttcaggta taattccttt tataaaaaat
     1681 tcacttgctg aaaattctgt cggtctgtta aatgtatttg atgtttctat gatttcacta
     1741 atcctatcca ctctgaaact acgtacctca tttctcatat tacaaaatgc aactacgtac
     1801 caattattat tccagtgaac aattccatat ggatttataa ttctatattt tgattttgct
     1861 ttacgacctg catggtagtt gatttctaca gatagctcct ttaatatact attctccaat
     1921 tttttaagta ctgattctat agacactttc ccaattggtt ttattacttc aagggctctt
     1981 aagtggtttg ttaaaatatc ttcttgtttt tgatttgaat acatttttaa ctttaatatc
     2041 gatttatcta atgcttcgtc taagaagtat ccttcttttt tagcaaatag agctgcatga
     2101 agtagagatt tttgctcttc tatatcaaaa aaaagaggtg ctttagtaaa attatttaaa
     2161 aggctatacc caccattatg acctgtatct gaaattatag gtactccact cgcagaaagt
     2221 acatcaatat atcggtacac tgttcttata tttatttcta atttttcagc tatctgtttt
     2281 gctgtaattt ttttgtcaga gtttaacatc cacagtatag acatcatatt gtcaatttta
     2341 gccatgtagt ttcacctctt tctatttgtt atataaagtt attatataac tttttgattg
     2401 attttttaca aaaaaacatt agtaaactgc taataattta tactaagatt tatttcaaat
     2461 atttatagat agtctttttc tatagttaag ttatccatta aattacatag tagtatatct
     2521 attttagttg atatattttc agctacctcc cttgaatatt attacaataa gtctaaatct
     2581 tctttcttcc ataaatatat tatgaggaaa gactatcatc tttaatggtt taatagtata
     2641 aaagatatta atttaaaaaa atctattttt atatcagatg gatattgttt tttataaaac
     2701 tgaaaaaatc acctaataga atctatgatt ttttaacata acctccataa attagtacca
     2761 attatcactt caaattcaaa tttttatctg aagaatatac aatcatattt ccatactcaa
     2821 cataatattg agtgtttggc ttgtatttac aaattattat tctaacaaaa acaactatac
     2881 atagattaaa atcatcttct aaacata
//
LOCUS       DAEMHZ010000239         2885 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000239 DAEMHZ010000000
VERSION     DAEMHZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.243"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(97..1773)
                     /gene="bclA2"
                     /locus_tag="KPK72_003750"
     CDS             complement(97..1773)
                     /gene="bclA2"
                     /locus_tag="KPK72_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG2490914.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2150..2689)
                     /gene="hpt"
                     /locus_tag="KPK72_003751"
     CDS             complement(2150..2689)
                     /gene="hpt"
                     /locus_tag="KPK72_003751"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2490915.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
BASE COUNT          696 a          675 c          436 g         1078 t
ORIGIN      
        1 taatcttttc gtcatataaa cctattaaat attatcctat ttattggtat atgaaatcat
       61 attgtttatt attttataat cttttcatca tataaactat tgtattctat aaactgatac
      121 atatccagct gcagaagtac ctgtcgtgcc tgaaccaaat gtaaaactta aagtaacagc
      181 tgctgctcta gcatcaacta aaaagcttaa acttacactt acatctgatg aagtaagtgt
      241 atttactgct tttgcaataa agttattttg agctactcca ttaaagttag tagtaattga
      301 cataaatcct gcaagtgaaa gtgtggcacg tataccgaaa gatacattgt atatccctcc
      361 aactgcaaga tttatagtac tacttgttgg aaaagttata ttagttccat ttataaatga
      421 tgaattaaat gtcactaatg tattattcac taaacttgaa gaggacactg taaattgtgc
      481 attgtttgct gctaacactc ctgttgctcc tgtcgcccct gttggacctg ttgctccatt
      541 tgctccggtt gctccggtcg cccctgttgc tcctgtcgct ccatttgctc cggttgggcc
      601 tgttggtcct atcgctcctg ctactccatt tgctcccgtt gctcctactg ctccggttgg
      661 gcctgttgct ccatttgcac ctgttgctcc tgtcgctcct gttgctcctg ctactccatt
      721 tgctccggtt ggaccggttg gacctattgg acctgctact ccattcgctc cggttgctcc
      781 tactgctccc gttgggcctg ttgctccatt tgcccctgtt gctcctgtcg ctcctgttgg
      841 acctactgct ccatctgctc ccgttgggcc tgttgctcct actgctccgg ttggacctat
      901 tgccccagct actcctgttg ctcctgtcgc tcctgttgga cctaccaaac catttgctcc
      961 ggttgctccc gttgggcctg ttgggcctac caaaccattt gctccggttg ctccggttgg
     1021 gcctgttggg cctgttggac ctaccaagcc atctggacct gttgctccta ctgctcctgt
     1081 tggacctacc tctccatctg ctcctgttgg acctgttgct cctattgggc ctgttgcccc
     1141 agttgttcct gttgctccag tcgctcctat acttcctgtt ggacctgttg ctcctgtatt
     1201 tcctgttggg cctgtagcac ctgttactcc atttgctccg gttgctcctg tatttcctgt
     1261 tggacctgtt attccatttg ctccggttgc tccggttgct ccagttagtc cggttggacc
     1321 tgttgctcct gttgctcctg tattccctgt tggtcctgtt attccatttg ctccagtcgc
     1381 tcctgtattt cctgttggtc ctgtagagcc tgttattcca tttgctccgg ttgctccttt
     1441 gtttcctgtt ggtcctgtta ttccatttgc tccggttgct cctgtattcc ctgtcggacc
     1501 tgttattcca ttcgctccag tcgctcctgt atttcctgtt ggtcctgtag aacctgttgt
     1561 tccatttggt ccagttgctc ccatatttcc tgttggaccc gttattccat tcgctccagt
     1621 tgctcccgta tttcctgttg gtcctgttat tccatttgct ccggtcgctc cggttgcacc
     1681 tctaggcccc gtcggtcctg ttggaccagt agcacctgtt ggccctgttg gacctacatc
     1741 ttgatataaa cttggacctg aaatatcact catataatta atcctccttt tttaaagtta
     1801 gagtattact ctataaatat tgagtattac tctatataat tatatgaatc gacaatttct
     1861 tatgtgataa tgtatctatt tgcttatatc aacatatatg ctttgattct tgaatttcat
     1921 tacttttttg taaaatcttt tacaattatt agtactcttt tacaattatt aatacaatta
     1981 aaaaactcat tttccatcaa ttatttaaaa tttttttatt atgttataaa aatagataat
     2041 taaattgcat aaaattaatt taatactact ttaattactt tgtaaataaa aaaacctcaa
     2101 cacgattaat aaaaatcata ttaaggcatc cacaaaatta tataattgct tatctatctt
     2161 catcagtaac attgaatata taaggaacat ttctataata atcaccaaaa tcaaacccat
     2221 atccaaccac aaacttatct tctatttcaa atccacaata atctggcact aaatcgactt
     2281 ttcttctgct tggtttatcc aaaagaacac agcattttaa actagcagga tttttagctt
     2341 ttagatgttt taacacaaaa tccatagtaa gtgcagaatc agtaatatcg tctacaacca
     2401 aaacatcata tcctgctata tcagtagtca catctgaaac tactttaacc cttccagtac
     2461 tttcttcatt gtttccataa ctagatgtag tcataaaatt aactctaagt ggtaaattta
     2521 tatttctaac taagtctgca caaaatataa agctaccttt taatagagaa actaccataa
     2581 gatttttatc tttataatct ttttctataa ttgctcctaa ttcccttaat ttatctttaa
     2641 ttttttcttc tgaatacaat acttcccatt tttttgtttc tatatccata aaaagacctc
     2701 caattgtaat aatccttctt aattttataa gagattgctc actttttcaa tactcttatt
     2761 caatattagt caatattatt gagtttttat gttcattcaa caattattaa caatgtatct
     2821 tatattttaa gtagttcata tatccacact aaattacata ctatatattt tagacaaaaa
     2881 aagaa
//
LOCUS       DAEMHZ010000240         2879 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000240 DAEMHZ010000000
VERSION     DAEMHZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.244"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..646
                     /locus_tag="KPK72_003752"
     CDS             14..646
                     /locus_tag="KPK72_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2490916.1"
                     /translation="MNVNSKSEDLSSSKTVARKFINKFMEKNKDFEVEEIDLYKEHIP
                     RLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAPMWSLSFP
                     APLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLKPVMNKGL
                     NYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            722..931
                     /locus_tag="KPK72_003753"
     CDS             722..931
                     /locus_tag="KPK72_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490917.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(1071..1637)
                     /locus_tag="KPK72_003754"
     CDS             complement(1071..1637)
                     /locus_tag="KPK72_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG2490918.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            1798..2451
                     /locus_tag="KPK72_003755"
     CDS             1798..2451
                     /locus_tag="KPK72_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG2490919.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSVPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(2609..2785)
                     /locus_tag="KPK72_003756"
     CDS             complement(2609..2785)
                     /locus_tag="KPK72_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490920.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         1072 a          311 c          405 g         1091 t
ORIGIN      
        1 aaaattatta tatataaatg taaattcaaa atctgaagac ttatcttcaa gtaaaactgt
       61 agcaagaaaa tttataaata aatttatgga aaaaaacaaa gattttgagg ttgaagagat
      121 agacctttat aaagaacata taccgagact tgagtatcag tactttgaaa agagaaatag
      181 catagttagt gaagaaaatg ctaaaaattt agacgataag gatagaaaag agcttacaaa
      241 gattagaaat ctttgtgacc aatttgtgag tgcaagtgta tatgtaatag cagcacctat
      301 gtggagttta tcttttccag caccacttaa agaatatata gattgtataa ttcaagatgg
      361 aaaaactata tcttttgaag gtaatgataa accacaagga attttaaatg atattgatag
      421 aagtatggta tatatacaat cttctggtgg acacatacca tgggtgttaa agccggttat
      481 gaataaaggg ttaaattatg tggaaagtat catgaaattt ataggaatta aaaaatttga
      541 tgaattgtta gtagatggaa ctggaactag tgaagaagaa agacaagctg caatagaaaa
      601 agcatctgaa aaaatagatg gtataataga tggtatgaaa ttttaattat aatattttat
      661 taaaaatata aaggaaaaac ctcttttaat gtagaaactt atattgaagg aggcgagaat
      721 catgcgagaa gaaaaaagta atgaaaagta tgattgttat tggtgtaatc aagaaaataa
      781 tttctgtgta gaaataaaag ataatatagt catgatagat gatggcactg gtacgttaaa
      841 acaagccgtt ttcatagggt ataaacaaat ccaaattaat ttaaattgtt cacattgtca
      901 aaacttaaat agaataaaat taaatttgta gaattttaaa atggaaatta ttgaaggaat
      961 taaaaataga aagtgttaat atttagactg aaaattatat tttatacaat aaaagtttat
     1021 ttaaataaca ttgtatttta atatttattt tcagtcttac tttattaaaa ttattttttg
     1081 gttatgtctg gaaaatatct gtctactaat ttttctgaaa tattttttaa ttggtcagcc
     1141 attgaagtca gacttaataa ggctatcaat aaatcatctt tattttctgg atttatacta
     1201 atattttcaa aattagactc tataaagtcc ggtatattct ttctttgatt ttcttttatt
     1261 aacatgaatt tttcgtaaca tgaaactaaa tctttttcac tgaaattatc cttttcatac
     1321 acaccatgta agattctttt aatttcttgt attgtaagtg tatttttcat agaataaata
     1381 attatcatct gtaaaatttg ttctttagta tacttttttc ccttaactgg tttgattaat
     1441 ccctctttgc tgtaattatt tatcatggtt ttagtaagaa ttttatcact ttcaaatctt
     1501 ttattaggag aaagtttatt gtcaattaga gaaataattt gatccatata taagtcaata
     1561 gagggaatat cgtttgaagt tatatctgca ttatttagtg tttctagtat tatagtatta
     1621 agttcattat tcatcatatg ttacctccat aatatatata atacactaaa ttagaaagta
     1681 aaacaatatt aagacttgta aaaaaaacat aaagtggtat aattaaatat ataatatagt
     1741 aatgaaaact atatttatga atctgatttt taaaatacat aattgtagga ggacgaaatg
     1801 tatcaatttt ttttaaaggg tagagaccca ataagcagtt taactcattt tataggtgct
     1861 tgtttatctc ttttagcaac tataatttta gtttttcaat ctgttacatt acaggaaact
     1921 tctctcttga tgatagtatc tgtatcggta tttggacttt cactaattgc tttatatagt
     1981 gcaagttcgt attatcattt tttaaagggg acaccagaac aagaattgtt ttttagaaaa
     2041 gtagaccatg caatgattta tgtattgatt gctggctctt atacaccaat atgtctgaat
     2101 tttatggaaa aaaaagaagg cataatattt gttacagcaa tatggatagt tgcatttatt
     2161 ggcataataa taaaaatatt ttggatggat gcacctagat ggttatctac cagtatatat
     2221 ttacttatgg ggtgggcaat tgtttttgat ataaatgcat tcaattcagt tcctaaggat
     2281 tgccttagac tattaattat ggaaggtgta tcttactcta taggtgcaat tatttatata
     2341 attaaaaagc ctaatataag tcctgaattt ggatttcatg aaatatttca tatttttatt
     2401 atgataggtt ctttatttca ctttttagca gttttattgt atgttttata gttttaaaag
     2461 atttgattaa ttgatactaa aattctacaa tgaataaaat ggaactgcct tatatagatt
     2521 atatcttcga caataagaca gttccttatt ataaatatta aattctactt ataataaaat
     2581 tttgataaaa atagtccatt caaaatgcct attccataat tcctttaatg tacttttcat
     2641 ttacgacata tagtttgaat ttatgtacta taattatatc ctctaaattc tctgaaacac
     2701 taacaattct gacacatttg tttctaattt ctacaggtaa atctaaatca tttgttaaat
     2761 tcacatagtc atcatattta tacataaaat catcccttta cattaagata tacttattat
     2821 actatatttt aataagtttt tttaaattat tacaattttt cccgaaattt catttttta
//
LOCUS       DAEMHZ010000241         2560 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000241 DAEMHZ010000000
VERSION     DAEMHZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.245"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            43..1854
                     /locus_tag="KPK72_003757"
     CDS             43..1854
                     /locus_tag="KPK72_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2490921.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQIENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(1942..2523)
                     /locus_tag="KPK72_003758"
     CDS             complement(1942..2523)
                     /locus_tag="KPK72_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490922.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          947 a          285 c          355 g          973 t
ORIGIN      
        1 gattatttgc aaattatata tattacaaat gaggtaacaa taatgaagaa aaaatatgat
       61 tttttaattt attctgctat ttggctagtt attattattc tttttatatt ttctctatat
      121 cacagtatag agcatattaa aattataagt cacactggta ctattcgtag tgaaactcaa
      181 aaggttgtta agcaagaatt gaataatgaa agaaatgatg acttgataaa acgactggat
      241 aatattctga taaagctaag aacaggtaat ggagaaaatg gatttcaaag atgtgataat
      301 aaagaatttc aacaaaaact aaatcaaatg gattctatgt gggaatctat gaagaaagaa
      361 attataaaag ttagaaatgg tgcttcaggt gataaattat ataaattgag tgaagagtat
      421 tctgtgttgt ctaatcaaat tgtttttata tctgaaaaac actctaatgc aaaattgtat
      481 tcttttgcta ctgcactatt tatttatctt atattctcaa caataagtct tttaatttgg
      541 gaatattata ataaaaaaag gtttaaaaga atattttata ctgataattt aacaaaaata
      601 aaaaatcagg ttgcatttga aaacagagcg attgaaattt tgcacaatgc atctaacaaa
      661 gagtatgtac tgctaaatat tgatattgac aattttaaat atattaatga tacacatgga
      721 tatgaatatg gagataaagt tctaataata gttgcagctg cactttctaa aacttttaat
      781 atcaaagaaa cttgtgcaag aattggttca gataattttg taatacttgc aaaatataga
      841 gattctcttt tagaggatat tcgggaaatg ctaactaatg ctataatctc tgaattagat
      901 atgaatgtaa ctcaaactat atcttattgt ataggagcgt acttagttga aattgataat
      961 ttaggttata aatccatcaa ttctatgatg gataaggcaa atattgcaca caaggtaagt
     1021 aaaacaagag gaatttcctc cacagtatgg tataatgaaa atctgttaaa acagttacaa
     1081 atagaaaata gtatttataa ttatatgtat aaagcgatag aaaatgaaga atttcatatg
     1141 tatttgcaac ctaaatttca aatatcttct ttaaatgttg tgagcgcaga agctcttgta
     1201 agatggtttt ctccagaact tggtttttta tcaccagatg aatttattcc attgtttgaa
     1261 aaaagtggat ttattattga attggacttt tatatgttaa aaaaagcgtg ttcatttgta
     1321 agaaaaacgt ttatgaaaaa aaatcaatat acatatccga ttgcagttaa tttttcaagg
     1381 gttactattt accaaaatag tttttatcaa aggtttttag atactgttaa agaatatgaa
     1441 attcctttta agtatataga gatagaggta actgaaagtg cttttaatga aatatctcaa
     1501 ccagttattt caattttaga ggaacttaaa aagttaggat ttttaatttc aatggatgat
     1561 tttggttcag gatactcttc attaagtctt ttatgttctc tatctattaa tggattgaag
     1621 ttagataaaa gcttacttaa agaaaccttt aaccgggaga aagtatatag tattattcag
     1681 tgtattattg aaatgtcaca tcgtataggc atgtctgttg tatgtgaagg tattgaaact
     1741 aaaaaagact tggaattttt aaatacagtt aaatgtgatg taggacaagg gttttatttt
     1801 tctaaaccga tagaagaaaa agaatttttt aacaaatatg taactaaaaa ataatataat
     1861 tatttttatc agtctataca ttgagtcatt ttatatctcg ttaaatgact caatgtactg
     1921 ttatgtattt taaaatataa attatttaaa acgttttttg tacttatata gaaattcttc
     1981 tatatttttt gattgttctc ttatttcaac tgcttctata tctagtaaat aaagtataga
     2041 atcatttgta agttcaacat attcaccatc ttctgaaccg agtgcatgtc tctcaatttt
     2101 taatcctttt tcttcataat atttttttgt ttctatatat agtgattctc gcttttgtat
     2161 gtagtctttc acttcattat ttggaaagtt atatgaatcc actaatgttt cgccatttat
     2221 aaaagttttc tcggtagcaa ctttttcatc ccaacaatgg atttcttccc aagtaaaaaa
     2281 tatctcagtt ccatctggaa tgaatgaaaa cctggtaata atatataaat aattattgaa
     2341 atttacttca taatattcag gaatatcttc aaattccata tagtaatttg tataattaaa
     2401 attactatta tcttttttta caaagggtat tttttcaata aatttatata ttgattgttc
     2461 atttttaaaa attcctatat cataactttt ttcaaattga ttaattttta ataaatacaa
     2521 cattctttct ccttttattc aaaatataat ttttataaat
//
LOCUS       DAEMHZ010000242         2538 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000242 DAEMHZ010000000
VERSION     DAEMHZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.246"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(243..998)
                     /locus_tag="KPK72_003759"
     CDS             complement(243..998)
                     /locus_tag="KPK72_003759"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2490923.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPERENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1438..2427
                     /locus_tag="KPK72_003760"
     CDS             1438..2427
                     /locus_tag="KPK72_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG2490924.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLIVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          931 a          311 c          376 g          920 t
ORIGIN      
        1 tatgattcca tataccaata aataggataa tatttaatag gtttatatga tgaaaagatt
       61 ataaaataat aaacaatata attccatata ccaataaata agataatctt taagaaaaat
      121 gattatattt aaaaggtaag cataaaattt ataaactttt agaatagaaa tctcaatcaa
      181 ttagatattc aaattgatga atatgaatac ctaaatagat tgagatttat tatgtacaaa
      241 acttatccta atatatctga cattttataa agcccagatt gcttaccaat taaaaattta
      301 gctgcaacta tagaaccgtt tgcaaatact tcatcagatt gagaatgatg gcttaatgta
      361 agaacttcat tatcacctgc aaatataaca tcatggtcgc tgattatatt gccacctctt
      421 atagagtgta ttccaatttc atttggtttt cttttacaat ctctaccata tctaccatgt
      481 gtattttctc tttcaggcaa gacttctcta attgcattag ctatcatagt agcagttcca
      541 ctaggggcgt cttcttttct attgtgatgt ttttctatga tttcaatatc aaaaccttct
      601 aacatctttg aagcatcctt aactaatttt attaatatat ttactccaag agatgtattt
      661 gatgataata atataggaat gttttttgat gcttcttcta ttttatttaa gtcattttcg
      721 tcatatccag tagtagctaa tactacaggt gtattatttt ttagagcata tgacaatatt
      781 ggctctaaag tctcatgatg tgaaaagtct ataattacat cagttttttc agttacatca
      841 gtcattgttt tgtaaagctt tgtatcacta tgatgacctt tttcatcagc agacctagct
      901 acaccacaaa ccaactctaa ttcattatct ttttttacac attctgctaa aactcttccc
      961 attgttccat tatagccact aattataact ttcaacataa attcctccta tgaaattaat
     1021 aaaaatttgt tatatttcta taagtttatt ttatcactaa tataatttat ataaaataat
     1081 attagtgata ataagcttac aatttatctt acattaaaaa atttaagtgg atttttcaaa
     1141 atatagataa atttcttttt cattgtttaa atgataaaaa tatatttgac ttcataccaa
     1201 aaagagtgcc ccatgaacta aaaagttaat tttgaaatac catattcata tatcatatga
     1261 tttatttgga aattaatatt aatacattaa aataactaaa tacatgtatg cataaagatg
     1321 tataaaaaat atataaacat atagtattaa ttttaaattc aacaatttaa ttgttaaaag
     1381 tgttattatt aaattagaaa tatcttaact taatatactg agatgggagt gatagatatg
     1441 ttaaaagaag aaaaaactta tcaagagaaa gacgcaaaag tattcaatgg agtggcagct
     1501 ttgataatga ttttattatt tttaatagtt tcaataggta tcatggtttt tgggatagta
     1561 aaacttgata gtggatttac tggaataggt tcacttatgt taattttggg acttgtattt
     1621 atagtagtag attttatcct attttttggg ctaaaaatga ttaatcctaa agaagctata
     1681 gttctagttt tgtttggtaa ctattatgga actataaaaa aagaagggta ctattgggtg
     1741 aatccatttt gttcagcaat aaacccagca gtatcaagaa ttaatgtatc gaaatcttca
     1801 tctgatgaaa aggtagatat atcaactaat tcaaggggca aaaaagtatc tttaaaaaca
     1861 atgactctta ataatgaaaa gcagaaagtc aatgatttat tgggaaatcc aattataata
     1921 ggagtggttg taatttggaa ggttatagat gcaacaaagg cagtatttaa tgtggataat
     1981 tataatacat tcttatctat tcagtgtgat tctactattc gtaatgtttc aagactttat
     2041 ccatatgatg tatctgaaga tggagatgaa aaatctcttc gtggtagtag tcaggaaata
     2101 gcagatagat taaaagatga attacaatca agggttgaca ttgcaggaat tgaagtgtgt
     2161 gaagtgagaa ttactcatct ttcttatgca ccagagattg cagcagcaat gttacaacgt
     2221 caacaagcag aggctataat agcagcacgt aaaaagattg ttgagggggc tgtaagtatg
     2281 gtagagatgg cattgactaa tttaagtgaa aataatgttg tgactttgga cgaagaacgt
     2341 aaagcagcta tggtaagtaa tctgttagtt gtgctttgtg gcaataaaga tgcacaacca
     2401 gtggtaaaca gcagttctat ttattaaatt ataaaaagtt taatagttta ttttattgat
     2461 atatagaaaa tttagtagtt cattttatta aattatagaa agattaatgg tttgctttat
     2521 tgaattataa aaagttta
//
LOCUS       DAEMHZ010000243         2425 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000243 DAEMHZ010000000
VERSION     DAEMHZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.247"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            348..2339
                     /gene="bclA1"
                     /locus_tag="KPK72_003761"
     CDS             348..2339
                     /gene="bclA1"
                     /locus_tag="KPK72_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG2490925.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECPPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEI
                     GPTGATGPTGVTGSIGPTGVTGPTGATGVTGSIGPTGVTGPTGNTGEIGPTGATGVTG
                     SIGPTGATGPTGATGVTGEIGPTGATGPTGVTGSIGPTGATGPTGATGVTGEIGPTGA
                     TGVTGEIGPTGVTGPTGVTGEIGPTGATGPTGNTGATGSIGPTGVTGPTGATGSIGPT
                     GPTGPTGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANGTFTVQEN
                     GVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYFAGFLFG
                     GTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          942 a          373 c          542 g          568 t
ORIGIN      
        1 cttataaaaa aatattgtta tggtaatata ttctatttac aattttataa ctttggttta
       61 caataatata gacaataaat cctaaaaaac ataataagaa aaaagtgctt aatattttag
      121 aaagactggt ttagtttaca ccagctaagc cagtcaagga ataatttaaa gtgtaatata
      181 taataatgaa aaagaacatg ctattagaaa tttctaatga catgttcttt ttttgtagaa
      241 atcagcatta ttatataaaa atttaatact ataaatttta attgtattgt aactaagcat
      301 gtattttaaa catagtatga tatagaccca aaatggaggg attataaatg agaaatatta
      361 tactttattt aaatgatgat acttttatat ctaaaaaata tccagataaa aactttagta
      421 atttagatta ttgcttaata ggaagtaaat gttcaaatag ttttgtaaaa gaaaagttga
      481 ttactttttt taaagtgaga ataccagata tattaaaaga caaaagtata ttaaaagcag
      541 agttatttat tcatattgat tcaaataaga atcatatttt taaagaaaaa gtagatattg
      601 aaattaaaag aataagtgaa tattataatt tacgaactat aacatggaat gatagagtgt
      661 ctatggaaaa tattagggga tatttaccaa ttgggataag tgatacatcc aactatattt
      721 gtttaaatat tacgggaact ataaaagcat gggcaatgaa taaatatcct aattatgggt
      781 tagctttatc tttaaattac ccttatcaga ttcttgaatt tacatctagt agaggttgta
      841 acaaaccgta tatacttgta acatttgaag atagaattat agataattgt tatcctaaat
      901 gtgagtgtcc tccaattaga attacaggtc caatgggacc aagaggagcg acaggaagta
      961 caggaccaac gggagtaaca ggcccaaccg gaagtacagg agcgacagga agcataggac
     1021 caacaggccc aaccggaaat acaggagcaa caggaagtat agggccaacg ggagtaacag
     1081 gcccaaccgg aagtacagga gcgacaggaa gtataggacc aacaggagta acaggtccga
     1141 caggaaatac gggagtgaca ggaagtatag gaccaacggg agcaacaggc ccaacaggag
     1201 tgacaggaga aataggacca acgggagcaa caggtccaac aggagtgaca ggagaaatag
     1261 gaccaacggg agcaacaggt ccaacaggag tgacaggaag tataggccca acaggagcaa
     1321 caggcccaac aggagcgaca ggagaaatag ggccaacagg agcaacaggt ccaacaggag
     1381 tgacaggaag tataggacca acgggagtaa caggcccaac aggagcgaca ggagtgacag
     1441 gaagtatagg accaacagga gtaacaggcc caacaggaaa tacaggagaa ataggaccaa
     1501 cgggagcgac aggagtgaca ggaagtatag gaccaacagg agcaacaggt ccaacaggag
     1561 cgacaggagt gacaggagaa ataggaccaa cgggagcaac aggtccaaca ggagtgacag
     1621 gaagtatagg accaacagga gcaacaggtc caacaggagc gacaggagtg acaggagaaa
     1681 taggaccaac aggagcaaca ggagtgacag gagaaatagg accaacggga gtaacaggcc
     1741 caacaggagt gacaggagaa ataggaccaa cgggagcaac aggaccaaca ggaaatacag
     1801 gagcgacagg aagtataggg ccaacgggag taacaggtcc aacaggagcg acaggaagta
     1861 taggaccaac aggcccaaca ggtccaacag gcaattcctc tcagccagtt gctaacttcc
     1921 tcgtaaatgc accatctcca caaacactaa ataatggaga tgctataaca ggttggcaaa
     1981 caataatagg aaatagttca agtataacag tagatgcaaa tggtacgttt acagtacaag
     2041 aaaatggtgt gtattatata tcagtttcag tagcattaca accaggttca tcaagtataa
     2101 atcaatattc tttcgctatc ctattcccaa ttttaggagg aaaagatttg gcagggctta
     2161 ctactgagcc aggaggcgga ggagtacttt ctggatattt tgctggtttt ttatttggtg
     2221 ggactacttt tacaataaat aatttttcat ctacaacagt agggatacga aatgggcaat
     2281 cagcaggaac tgcggctact ttgacgatat ttagaatagc tgatactgtt atgacttaaa
     2341 acgtgtctaa aataatctta aaaactattt aggctttatt taaatgacaa aagtattttt
     2401 atatattgag ttttacctat tttag
//
LOCUS       DAEMHZ010000244         2300 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000244 DAEMHZ010000000
VERSION     DAEMHZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.248"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            316..991
                     /locus_tag="KPK72_003762"
                     /pseudo
     CDS             316..991
                     /locus_tag="KPK72_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            1632..1877
                     /locus_tag="KPK72_003763"
     CDS             1632..1877
                     /locus_tag="KPK72_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2490926.1"
                     /translation="MIPRLILASSLVIIIVFIKIIKENKLKLQNEVIKNKLDIQYSHY
                     LSIQESHAKVKKLYHDINNHIYCIENLKYKKGKLMGM"
     gene            2053..>2300
                     /locus_tag="KPK72_003764"
     CDS             2053..>2300
                     /locus_tag="KPK72_003764"
                     /inference="COORDINATES: protein motif:HMM:NF025854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2490927.1"
                     /translation="MCKYLDNAIEACDKIHDKSIDKYIQIKGTISKSYFVIKCENSKI
                     NSIRKNKSLLLTDKKDKLVHGIGIQSIKSSIKKYRGDL"
BASE COUNT         1058 a          198 c          285 g          759 t
ORIGIN      
        1 ttatatatga aaaaaaatgc tgaattatat gaaagtagag tggaaactat actactaaat
       61 attagagaat atgtagattt agatgagttt gaattattta taatagataa gctaatacaa
      121 aaaattaaat aataaatcta atcgtaggtg ttatccctat gaatcgtatc caccattgaa
      181 atccacgaat acctatatcc aattggatat aggtattttt atacattttt agtttaaata
      241 ttattgataa atacatttaa aaagttttaa cctaaagtga agaatctaaa taaaaataaa
      301 atattaggag aggttatcat tacaataaca gcttgtgatg atgaaaaaga aatacaaatc
      361 gtaataaaaa atcaagttga taatatatta aaaaataaaa acaaagaata ttgaattaaa
      421 aaaatatagc tcaaaagaag aaacattaca aagttaattg agaggataca ctactcttag
      481 atataaaaat ggcagaacta aatagtatag atatcgcaag aaacataaga aaagtagata
      541 ataatattta taacatctct aataaattat gtacaggaaa gatatgaagt aagagcttat
      601 tgatacttat taaagccaat aaattttcaa gaactagaaa aacacatatt aacatgtacc
      661 aaaaaaattg aaaaaaataa aaataaatct aacacataca aaatctaatc tagcaaaaaa
      721 atatatagaa gttcaaaaaa agaacatgct aatacataca aaaaataaaa cttttgatgc
      781 tatatgtatc ctagaaaaaa tagaaaaaga tttaaatata gaggaacttg taagatgtca
      841 taaaagttat ataattaact taaactatgt agaaaatata aagtcaaata ctgcaatatt
      901 agacagtcaa gaagaagtac ctattagcag atatagatat aaagatataa aagagaggtt
      961 tctaaaattt attggtgata gaatatgcta aaccatataa ataaaatatt attaatatta
     1021 tcattgttta caccaataat gatactaaaa agtatattaa cttacgataa agatacagat
     1081 aagactaaga aaatatcttt ttttactgta ttattagctt atcttataac taaaatcatt
     1141 atattattta acgttgataa gacccataat tataatatga agaaaaatct atatgatata
     1201 atctattatt tgatatttat tatttgagaa cttactataa ttatttttat aagataggaa
     1261 ttgttaaata tatagtttca tttttcttat ttgacgtata tcacccttta ataaaagatg
     1321 ttatatctat aaatttattt tttgtagtta caagtaatgt aatatcatca gaggaagctc
     1381 tattagtgtt tagttatgat aatgctaaag tagatatata ctattatctt ataataataa
     1441 ttattatata gtagcttgtg ctatatttaa atttattaat aaaagattgg gactagataa
     1501 agataataaa ataaactata tatacttatc tgttgcttta ataacaaatt tatttactat
     1561 attaataatt tatataaata aaaaaatgta tcgatttggt gggtattagg aaatatatag
     1621 ttgggtatat tgtgatacct aggttaatac tagctagtag cttagtaatt attatagtgt
     1681 ttataaaaat aattaaggaa aataagttaa aattgcaaaa tgaagtaata aaaaataagc
     1741 tagatataca gtattctcat tatctaagta ttcaagaatc acacgctaaa gttaagaagc
     1801 tttatcatga tataaataat catatatatt gtatagaaaa tctcaaatat aaaaaaggaa
     1861 aattgatggg tatgtaaata gtttaaaaga ttaaattaaa gaatttaaat caatttacaa
     1921 tacaggtaat gttagattgg atataataat aaataaaaaa agtgatgtaa gtgaaaaaag
     1981 aaaaattaaa ttcatttgtg atatcaattt ttccaaagtg aattttataa aaccaataga
     2041 tgtatctaat atatttgcaa atacttagat aatgcaatag aagcttgcga taagatacat
     2101 gataaaagta ttgataaata tatacaaata aaaggaacta tctcaaagtc ctatttcgta
     2161 attaaatgtg agaatagtaa aataaattca attagaaaga ataaaagttt attattaaca
     2221 gataaaaaag ataaacttgt acatggaata gggattcaaa gcatcaaatc ttctataaaa
     2281 aaatatagag gagatttatt
//
LOCUS       DAEMHZ010000245         2286 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000245 DAEMHZ010000000
VERSION     DAEMHZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.249"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..909)
                     /locus_tag="KPK72_003765"
     CDS             complement(<1..909)
                     /locus_tag="KPK72_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2490928.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIKLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVIVTTNVDNKAKFDVITLLDNKKVDTQIAKVITELQDNDYV
                     TWKKEATLEATAGLTFTNGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDKDLAESSLIYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLE"
     gene            complement(910..1086)
                     /locus_tag="KPK72_003766"
     CDS             complement(910..1086)
                     /locus_tag="KPK72_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490929.1"
                     /translation="MSKILSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(1079..1516)
                     /locus_tag="KPK72_003767"
     CDS             complement(1079..1516)
                     /locus_tag="KPK72_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490930.1"
                     /translation="MLNNIIDGISVKLDKSFGENYTIYSEDVEQGISEPCFFIVPINP
                     SKVPYPSGRTLKKNSFDVHYFPKSNDKSFEINEVAEILLEELEYIEIEGDLVRGTNMN
                     YEIIDNVLHFFVDYNYFTIKSNNTDKMDTVELFGGLKRGDNFE"
     gene            complement(1509..1934)
                     /locus_tag="KPK72_003768"
     CDS             complement(1509..1934)
                     /locus_tag="KPK72_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2490931.1"
                     /translation="MARWGSVDFREFKRACRRMEKFTKIDLDKFCKDAARELAARLLS
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(1934..2281)
                     /locus_tag="KPK72_003769"
     CDS             complement(1934..2281)
                     /locus_tag="KPK72_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490932.1"
                     /translation="MMNKTRKAIEMLYRDKCTIVEYQPVKDPVTKRTNNKEVVVLENQ
                     PCKLSYKNITSTTDGKVAKLEQTIKLFISPNINIKAGSKLIINDKEYVKSGESAMYPN
                     HQEIILELFKDKA"
BASE COUNT          686 a          466 c          234 g          900 t
ORIGIN      
        1 ttcaagctgg atttgagtat agttaacatc aacatcaaac tcaccatcat actttttatt
       61 agtattagat ttatttatat cacatccagc tatagctcca gtagtccagt aaattagact
      121 agactctgct aaatctttat ctttaatctt attctcaaca gatactacac cctcataatc
      181 tgcatcattc tttttatata aaacagtttg aaacttagcc ccaactttgt ctctcattct
      241 cttagtaaac tccacaaaca aacttttaat ctctgctgtt gtagccaaac accctagtgc
      301 attaaatgag taactttcta tcttatcaag aaaagcttga tactctgctc ctgtcacagc
      361 ttcaccatta gttccattag taaaagttaa tcctgctgta gcctctaatg ttgcttcctt
      421 cttccaagtg acataatcat tatcttgcag ttctgtaata actttagcta tttgagtatc
      481 aactttctta ttatctaaaa gtgttataac atcaaattta gctttattat caacattagt
      541 tgtgactatc acctttaagt cattacctct tatcccacta tatttagctg tagcaatagt
      601 acaactagct ttaacacctt tatttaattt atagaaatat cctagcttta tatttttaaa
      661 taaatctctc aaacctttca acttctcatg tgtataatca tatccaaaat acttagttga
      721 atacttctca aaatcatcac tagtcacttg aaatacttct tcatcaattc cccaatcaag
      781 ttctaaaggc attgcaacaa tacctctatc gctaagtgaa ctggttgccc ttgtggcact
      841 tacaaagttt atatagctac ctggtaatac tttattctgt gttacaaatg ttcctccacc
      901 taaagccatc taactcactc ctttcatgaa tttatttatt ctatcctcta cctctgagaa
      961 ggaatacaat tcattttctt ttaaaattgc atttaataag tcttttctat ttacatactt
     1021 cttagaatta actatttgct ccttagtaaa cttgtaatca tcttctttgc ttaatatttt
     1081 actcaaaatt atcacctctc ttcagaccac cgaataactc tactgtatcc atcttatctg
     1141 tattattact ttttatagtg aaatagttat aatcaacaaa gaaatggagt acattatcta
     1201 taatttcata attcatattt gttcctctaa ctaaatcccc ttcaatttct atatactcta
     1261 attcctccag taaaatttca gctacctcat ttatttcaaa tgatttatca ttactttttg
     1321 gaaaatagtg tacatcaaag gaattctttt ttagtgttct accacttgga tatggtactt
     1381 tacttggatt tataggaaca ataaaaaaac aaggttcact aatcccttgc tccacatctt
     1441 cactataaat tgtataattc tctccaaatg acttgtctag ttttactgat attccatcaa
     1501 ttatattatt aagcatcaaa cactccttta agcaatatta atagtttttt ctcaataatc
     1561 ttgtcaactt gactttgtag ttccatctct gaaatagtta agaaatgttg tcctttaacc
     1621 catcccttac catctttagt tctatgcccg aaatttacat aacttgcata ttcagtcgga
     1681 ttaacaactt ctataatata attatttcct tgtttataca caggaagcga cctagcataa
     1741 gccactccat tccatccttg tcgtaagaat cctgtatcaa ctggtgttct tctaattact
     1801 ttactaagca gtcttgctgc taattctctt gctgcatctt tgcaaaactt atctaaatca
     1861 atctttgtaa atttctccat tcttctacaa gctcttttaa actctctaaa atcaacacta
     1921 ccccatctag ccattaagct ttatccttaa ataactcaag tattatttct tgatgatttg
     1981 ggtacatagc tgattctcca ctctttacat attctttatc atttataata agttttgaac
     2041 ctgctttaat atttatgttt ggagatataa agagtttaat agtttgctct agtttagcta
     2101 cttttccatc tgtagttgat gtaatatttt tatatgaaag tttacatggt tgattttcta
     2161 atacgactac ttctttattg ttagttcgtt ttgtcacagg gtctttaact ggctgatact
     2221 caactatagt acatttatct ctatatagca tttctattgc ttttctagtt ttattcatta
     2281 tttcca
//
LOCUS       DAEMHZ010000246         2273 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000246 DAEMHZ010000000
VERSION     DAEMHZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.250"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            210..2114
                     /locus_tag="KPK72_003770"
     CDS             210..2114
                     /locus_tag="KPK72_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2490933.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVFATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDGEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT          952 a          236 c          420 g          665 t
ORIGIN      
        1 atgttagaat aagtcttatt tgaaaatttt agtgtattag agtaagtatt attttactag
       61 taaaaaatat tgagaactat atgtagattt gcttaaaaag gaatttacat atagttcttt
      121 tcgtataata aatagtacaa tcttatatgg taaagataaa gtgtgatatt aaattttatt
      181 atcatccaaa atctaaggag gaatctctta tgaaaataaa atttattata gtagcatcaa
      241 taatattttt aatagtatta cttgtggggt gtcaaagtac taaagacaca gaaatcaatg
      301 tattcgccac aagtgattta cattcaatta ttccagataa tttaatttcc tatgtagaag
      361 aggagagaaa atatgatgag aatctactta tggtagatgc aggagatttt tttgacatgc
      421 aaagtggtga gatgaataag tggtttactg gtcaaaaatt agtaagattt aaagatggga
      481 taccagagta caaaaaaata gctgaaccac gtgaaggaga agttccattg gctaaaaaaa
      541 tggcaaagct gaagtatgat gcagttacat ttgggaatca tgaatttgta tctaatgaca
      601 agcagagcct tgataaattg gtttctgatt ttaaaaataa caatatacca ttagtatcag
      661 caaacgtata tgaacaatct ggagaaaact acgtaaagcc atatgtaatg aaaaatataa
      721 atactgacaa gggaactgta aaggttggta tacttggtct gactataaaa gaagtagggg
      781 aacgttcaag gtttaaaagt tttgaacaag ataaaaaggc tagagaactt ggggaacaac
      841 ctcaatacaa agggaaatta tatgcaaatg atttagtaga agatgcaaat aagtgggtaa
      901 aggttatgga aaaaaaagaa aagccagata taatagttgc cattgtacac tcaggtgaag
      961 agccaaaaaa tcctaaaaat ccaggaaata gaataaaaga actagcaaca actgtagagg
     1021 gaatagatgc tattgtagca ggtcatacac atgaaaaaat agaacagcat acatataaaa
     1081 ataattctgg tgaagaagta atagtaacac agcctggagc tcatggtaat agtatatcaa
     1141 agattaattt taagttggat aagaagaatg ataagtggtt tataaaagac aaatatagtg
     1201 aattaaaggt ttttggaaag gaagtaaata taattacaac ttctggtctg aatccaaaat
     1261 tttcagatga aatggcagtt aatttgttta atgaaagaga tagagatgtt caacctatat
     1321 ttatagatac tggagatttt ttaaatacta atacaaaaga aatgactaag tgggctaaag
     1381 agtggcaata caatgaaaat aaaggcatct ataaaaaaat aaatgaatgg ccaattgtat
     1441 atcttggtta tgatgctgta gtacttggta gccatgaatt tacagaggat aaagagactt
     1501 ttgaggaaaa aaaatctaca ttggattata ttatagaaaa ctttgaagaa atgaagatac
     1561 cagtattatc agctaatata tatgatgaat ctggagaaaa ttatgtaaaa ccttatataa
     1621 ttcataatgt agaaactagg gaaggaaata taaaggttgg agtattaggt cttactacta
     1681 ctgataagga tggagaagac ttaagtagct caagcttaag caaagactca aagacttcga
     1741 aaattcctga agaaacagga aagttatata ctaacaatct agtaaaagat gcaagcgaat
     1801 gggtaaaagt tatgcaaaag gaaagtcctg atgtgattgt tgcagttgta cattcagata
     1861 ataatagtac tctaaaaaat ccaagtagtg aagtcaaaaa acttgcaatg tcagtagatg
     1921 gaatagatgc tattgttgta ggtcatacga aaaataaggt agaagaacat atttacaaaa
     1981 ataagtctgg ggatgaggtt atagtaacac aatctggaga ggattgttat tcaaaaataa
     2041 gtcttgaact aagaaaagaa aatggcaagt ggaatatcat aaacaaatat agtaaagcaa
     2101 taaaaatgaa gtgaatttat tgatatataa accattatta atactgattc tacaatataa
     2161 ttaacactga tttttataat ataataaata ctgattctat aatataatta acactacttt
     2221 ttataatgtc atcaacactg attctataat ataattaata ctactttttt aaa
//
LOCUS       DAEMHZ010000247         2227 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000247 DAEMHZ010000000
VERSION     DAEMHZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.251"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="KPK72_003771"
     CDS             complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="KPK72_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2490934.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSYGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          739 a          372 c          245 g          871 t
ORIGIN      
        1 ttaataattc ttttaaatat aaaaaggaat tgcctatata atttaaaggc aattccataa
       61 ttatcaatta gtttttatta ttatgcagtt ttactgcttt tagatggaat aaagtttcct
      121 attattcctg ctattacaac tggaactatc cagttaaatc ctaatgaagc aaatggtaag
      181 ctattaacta gaggaatttt tacaccataa cttgttgcaa ctgttaatac acttactaat
      241 aatgccatgt atgcagcaca tttgaacacg ttgtcatttt taattttatt actaaataat
      301 gctagtataa ctagagttat agttgctgga tataccatac ttagtatagg agaagagaat
      361 tgtattattg tacttactcc aaagtttgat atgattgcac taaatacaca aactactata
      421 actatatgct catactttaa ttttccatct gtaagtgttg agaaatattg accagctgct
      481 gaagaaagac ctatagctgt tgttaaacat gctaatgcaa ctataatagc caataaaatt
      541 ttacctgtag ttccaagtaa agctgctgtt attgcaacta ttaaagctgt ttgttgaaca
      601 tcttgtccat attgttgaga cacagttgct ccaagataac ataatccacc ataaactaat
      661 gccattccta ttactgcaat tacacctgca cttattgtgg ctttcatctt ttctttctca
      721 cctgtataac ctttagctat tactgacatg attataattg atgcaaatac actagctgcc
      781 aatgcatcca ttgtttgata accttgagtt ataccttctg caaatacatt ttgtattaaa
      841 gaactgtctc ttatttctcc taatggagat acaatacctt taactattaa tactgccaaa
      901 gctataagta aagctggagt taatattgac ccaactatat ctacaacttt tgatggtctt
      961 attgttaaaa ccaatactag agcaaagaat actatagaga atattactgg gcttattcca
     1021 gaacctattg taggtaatat acccatttca taagttgttg ctgctgttct aggtattgct
     1081 aaaagtggcc ctatacagat taccattgca catcctaatg caatacttaa tcctttgcct
     1141 gctcttgaga acatcttcga tgtgtctcca ttgaacttag ttgctgctat aactgctaat
     1201 aatgccaaac caccatctgc aaaaaggaat cctatgaaag ctactagcca actacttcca
     1261 gataaaactc ctagatatgg agggaagatt aagttccctg caccaaaaaa cattgcaaat
     1321 agtgcaaatc caattacaat tacatcttta aaattgattt gtttctcacc catttaaagc
     1381 acccccattt gaattttact accttttact aaaaaataag tccaatctct taattaaatg
     1441 aaaacctttt gacgagattt tatttaatta tacatttttg acttaaattt gtcaattatg
     1501 tttcataatt tataaaatct tcacaatatt taatacaact ttcttaatta aacaaaatca
     1561 acctgtatac tttaaacata gttggttttt tgttttttct ctaatttttc ttgataaaat
     1621 taactattcc gaataattaa aatattaaga ctttttagat tattcgattt attattaagt
     1681 tgtattgctt tatatactgt atatatcact ctcctttttt acattcatat aatcttttta
     1741 attcaattta aattttaaat attttgtgtt tattatataa tctgaaaaaa aagcagtcaa
     1801 tagcattttt aattttttta attttcaaaa ttatgattct taaaaaatcg ttttcctttt
     1861 atcataacac ctatttacgt taattcttgt agtattttgt atttattttg ctaaattgac
     1921 agttaacttt attcaatttt tattctactt gcgtagattt gcctcttgtt ttttactctt
     1981 aaaaccattt tttctgaaaa ttccttatta tgatttatca acttattttt cttcataaca
     2041 attactctct aacttccaac ttacttaaac tttgcagttg ctagtattca cgtttacata
     2101 cttatatgtc ataattaact aaaaattctt aatatatctc tcaatgaaaa caatttactc
     2161 atttatttaa tacatttata taatcatatg tcttaaattt tgttttatta ttttattatt
     2221 gtcacaa
//
LOCUS       DAEMHZ010000248         2104 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000248 DAEMHZ010000000
VERSION     DAEMHZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.252"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..864
                     /locus_tag="KPK72_003772"
     CDS             <1..864
                     /locus_tag="KPK72_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2490935.1"
                     /translation="LIISILIGVLVKEYSSREIKKDDINVSKYIKYADLASKNNAQVN
                     WKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDILDELEFSNRQK
                     RLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILPSITIAQAIIE
                     SSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIYDDKNESIKDH
                     AKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAARLIELIQQYNL
                     QLIDSEIQSEM"
     gene            1303..1794
                     /locus_tag="KPK72_003773"
     CDS             1303..1794
                     /locus_tag="KPK72_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490936.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSF
                     KIE"
     gene            1822..>2104
                     /locus_tag="KPK72_003774"
     CDS             1822..>2104
                     /locus_tag="KPK72_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490937.1"
                     /translation="MRVTKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRID"
BASE COUNT          902 a          195 c          312 g          695 t
ORIGIN      
        1 ctgataatat caattttaat aggtgtatta gtgaaggaat attcatctag agagataaaa
       61 aaggatgata taaatgtcag taagtatata aaatatgctg atttagcaag taaaaataat
      121 gcacaagtta actggaagta tgtagcttcc attgttgctg tgttaaataa aaataactta
      181 aaaaacgtta aggattcaca gatacaagaa gtatcagatt tatttgttaa gaatttttct
      241 aaaaataata aaataaataa gttaagtgat attttggatg agttagaatt tagtaataga
      301 caaaaaagat tggttgataa ttatatagac aatcttaagg attatggtat aaaaccagaa
      361 agattgaagt ctgatacaaa atatatgaag tttattgatg aaattaaaac agaagcaata
      421 caaaattata aggattataa aatacttcca tctataacta ttgcacaagc tataatagaa
      481 tctagctggg gcaagtcaac tttggcaaaa caatataaca atctatttgg tataaaggct
      541 gacgcttatt ggaaaggcaa gtcagtgact ttagaaacga aagaacattt agatacaatt
      601 atagatgata aatttagaat atatgatgat aaaaatgaat ctataaaaga ccatgcaaaa
      661 tttttagcca caaataaaag gtataaaaac aatggtgtat ttgatgcaaa aacgtatata
      721 tatcaagcta aagcattaga aaaggcaggt tatagtactg cgaaggatga aaatggcaat
      781 agtatatatg cagctcgtct aatagaatta attcaacagt ataatcttca attaatagac
      841 agtgaaattc aaagtgaaat gtaactttga ttaaattttt attgataagg ctatgatttt
      901 taaaatcata gccttatttt gacatgcttt tacttgtaat tattaaatcg atatcattat
      961 ctttcaaaat ttactttata ctgtaaatca aattatttaa gttaaaactt ggaattattt
     1021 ataaaaggat tatttacttt atctaaattc tgcacttaaa gaacaccttt tcccttaagg
     1081 ctgaccgtta gtctataaaa attataatta cttataaaaa agtgaataga tatatataaa
     1141 gttttcagaa agacttttac ttgtaaattg aaatcaatga agtaaaaaag gattctttct
     1201 taaaagtaaa taacaactag atagtgaata ataaaaaatg gtaatttgaa ataattttta
     1261 tgaatagtat taatatatag atacagggag attgatgcga ttatgtatga aaataataag
     1321 gtaatataca accttgaaag atgtgaaaaa ctagaaaaga ctctttattt atctcaattt
     1381 aatacatata aaaattttaa attagcttct aaaaaaactg taataaattc attagagaca
     1441 tatgtttaca atatgaatat taacaatata agtcttgttg atactataga atgtatatca
     1501 ttagaagatg aacaacttac aggaaagaag ttgattttag ttggtgatat agatattgat
     1561 gcaatattag attatgcagg aaataaaaga aatagaaatt ctagtaaaaa aaatacattt
     1621 aaattaaaaa taccctttag tacatttata caaatgccta gaaagataga aaataaagat
     1681 aaaattaatt taaagtatct tatacaagat attacttcat ctattttaga tgataattta
     1741 tttattagtg ttactgcaat tataagttat gaaaatagtt tcaagataga gtaatcttta
     1801 aattatagaa aggtggatgc aatgagagta acgaaaacta cttttaatga tgtattttca
     1861 aatgatgcaa atggaagtat tataaaagta gaaaaatatg ttgataaaaa atgtgcatct
     1921 gtatttgaga ttttgaccta cactattatt gttacaaata caagtaggta caaaactgga
     1981 aatatatttt tcaaagatta tatttctaaa tatatagagt ttataaataa tactgtaaaa
     2041 gttaatggca ttataaaacg tggattagac ccacagcaag gtttttacat agggagaata
     2101 gatg
//
LOCUS       DAEMHZ010000249         2033 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000249 DAEMHZ010000000
VERSION     DAEMHZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.253"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>2033
                     /locus_tag="KPK72_003775"
     rRNA            <1..>2033
                     /locus_tag="KPK72_003775"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          547 a          436 c          608 g          442 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tactaccgat aaccgtttga gagaagggat
      781 gacacagtag gataagctaa gcacactgtt ggttatgtgt gcccaagcat tgaggcagtc
      841 aaagtaggca aatccgcttt gataatgctg ggatgtgatg gggagcgaaa tttagtagcg
      901 aagtagctga tttcacactg tcaagaaaag tctctatcga ggttaaaggt acccgtaccg
      961 caaaccgaca caggtgggtg aggagagtat cctaaggcca gcgagagaac tgttgttaag
     1021 gaactcggca aaatgacccc gtaacttagg gataaggggt gccaccatca ggtggccgca
     1081 gagaataggc ccaagcgact gtttaccaaa aacataggtt tctgctaagt cgcaagacga
     1141 tgtataggag ctgacgcctg cccggtgctg gaaggttaag gggatctgtt agagcaatcg
     1201 aagcagtgaa cttaagcccc agtaaacggc ggccgtaact ataacggtcc taaggtagcg
     1261 aaattccttg tcgggtaagt tccgacccgc acgaaaggcg taacgatttg ggcactgtct
     1321 caacaacaga ctcggtgaaa ttgtaattcc ggtgaagatg ccggatacct gcgacaggac
     1381 ggaaagaccc catggagctt tactgtagct tgacattggg tcttggtact acatgtacag
     1441 gataggtggg aggctttgaa accaggacgc cagttttggc ggagccatcc ttgggatacc
     1501 acccttgtag tactgggact ctaaccatag gccatgaatc tggtcttggg acactgtcag
     1561 gtgggcagtt tgactggggc ggtcgcctcc caaaaggtaa cggaggcgct caaaggttct
     1621 ctcagtacgg tcggaaatcg tacgtagagt gtaaaggcaa aagagagctt gattgcaaga
     1681 catacaggtc gagcaaggat gaaaatcgga cttagtgatc cggtggttct gcgtggaagg
     1741 gccatcgctc aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtc
     1801 cacatcgacg gggaggtttg gcacctcgat gtcggctcat cacatcctgg ggctgtagta
     1861 ggtcccaagg gttgggctgt tcgcccatta aagtggtacg cgagctgggt tcagaacgtc
     1921 gtgagacagt tcggtcccta tccgtcgcag gcgtaggaaa tttgagaaga cctgtcctta
     1981 gtacgagagg accgggatgg acgtacctct ggtgtaccag ttgtcctgcc aag
//
LOCUS       DAEMHZ010000250         1987 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000250 DAEMHZ010000000
VERSION     DAEMHZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.254"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..200
                     /gene="rrf"
                     /locus_tag="KPK72_003776"
     rRNA            84..200
                     /gene="rrf"
                     /locus_tag="KPK72_003776"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..281
                     /locus_tag="KPK72_003777"
     tRNA            207..281
                     /locus_tag="KPK72_003777"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Asn,seq:gtt)
     gene            286..360
                     /locus_tag="KPK72_003778"
     tRNA            286..360
                     /locus_tag="KPK72_003778"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            366..441
                     /locus_tag="KPK72_003779"
     tRNA            366..441
                     /locus_tag="KPK72_003779"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            447..523
                     /locus_tag="KPK72_003780"
     tRNA            447..523
                     /locus_tag="KPK72_003780"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Asp,seq:gtc)
     gene            534..608
                     /locus_tag="KPK72_003781"
     tRNA            534..608
                     /locus_tag="KPK72_003781"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Thr,seq:tgt)
     gene            623..707
                     /locus_tag="KPK72_003782"
     tRNA            623..707
                     /locus_tag="KPK72_003782"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Tyr,seq:gta)
     gene            717..790
                     /locus_tag="KPK72_003783"
     tRNA            717..790
                     /locus_tag="KPK72_003783"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Gly,seq:tcc)
     gene            801..877
                     /locus_tag="KPK72_003784"
     tRNA            801..877
                     /locus_tag="KPK72_003784"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Arg,seq:tct)
     gene            887..962
                     /locus_tag="KPK72_003785"
     tRNA            887..962
                     /locus_tag="KPK72_003785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Gln,seq:ttg)
     gene            974..1049
                     /locus_tag="KPK72_003786"
     tRNA            974..1049
                     /locus_tag="KPK72_003786"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Lys,seq:ttt)
     gene            1052..1140
                     /locus_tag="KPK72_003787"
     tRNA            1052..1140
                     /locus_tag="KPK72_003787"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:Ser,seq:tga)
     gene            1158..1248
                     /locus_tag="KPK72_003788"
     tRNA            1158..1248
                     /locus_tag="KPK72_003788"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ser,seq:gct)
     gene            1257..1333
                     /locus_tag="KPK72_003789"
     tRNA            1257..1333
                     /locus_tag="KPK72_003789"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1291..1293,aa:Pro,seq:tgg)
     gene            1419..1494
                     /locus_tag="KPK72_003790"
     tRNA            1419..1494
                     /locus_tag="KPK72_003790"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Trp,seq:cca)
     gene            1555..1631
                     /locus_tag="KPK72_003791"
     tRNA            1555..1631
                     /locus_tag="KPK72_003791"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Pro,seq:tgg)
     gene            1638..1714
                     /locus_tag="KPK72_003792"
     tRNA            1638..1714
                     /locus_tag="KPK72_003792"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1672..1674,aa:Ile,seq:gat)
     gene            1718..1793
                     /locus_tag="KPK72_003793"
     tRNA            1718..1793
                     /locus_tag="KPK72_003793"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Phe,seq:gaa)
     gene            1801..1877
                     /locus_tag="KPK72_003794"
     tRNA            1801..1877
                     /locus_tag="KPK72_003794"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1835..1837,aa:Met,seq:cat)
BASE COUNT          519 a          452 c          530 g          486 t
ORIGIN      
        1 taactctaaa aaaaataaaa aaagtattga caaaaatagt aaaaaatgtt aatattatac
       61 ttgtcctagc aacaagaaaa aaatgtggtt ataatagcgg agaagataca cctgtttcca
      121 ttccgaacac agaagttaag ttctctagcg ctgatggtac ttggtgggaa actgcctggg
      181 agagtaggac atagccacgt gaatgttcca aggtagctca acggtggagc aaccggctgt
      241 taaccggtag gctgtgggtt cgagccccac ccttggagcc attatggctc gttggtcaag
      301 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      361 tttgtgggat catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      421 cgagccctgt tggtcccacc atttgcggcc tggtagttca gttggttaga atgccagcct
      481 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaaataaa aatgctggtg
      541 tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac
      601 cagctccatg taaaactaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa
      661 atctgttgtc atcgacttcg gtggttcgaa tccaccatcc tccaccaatt aaatatgcgg
      721 gtgtagctca atggtagagt tctggccttc caagccagct gtgagggttc gatccccttc
      781 acccgctcca aagaaagcgt gggtgcatag ctcagctgga tagagcaacg cccttctaag
      841 gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg gattagccaa
      901 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      961 caaataaaaa tatggtctat tagctcagtc ggtagagcac ctgactttta atcagggtgt
     1021 cccgcgttcg agtcgcggat agatcaccaa tggagaggtg tccgagtggt ttaaggagct
     1081 ggtcttgaaa accagtgact tcgaaagggg ccgtgggttc gaatcccacc ctctccgcca
     1141 aaaaaatgac tgaatttgga gaagtactca agtggctcaa gaggatcccc tgctaaggga
     1201 ttaggctggg taaccggtgc gagggttcga atccctcctt ctccgccatt caaaaacgag
     1261 gtgtagcgca gtttggtagc gcacatggtt tgggaccatg gggccggggg ttcgagtccc
     1321 ttcacctcga ccaacttaag cttttagttc agttggttag agtgcccaac tcatggctag
     1381 taattctagg gtttgagtcc atgaaggtta ttcttcatag gggtgtagct cagttggtag
     1441 agcgtcggtc tccaaaaccg tgcgccgggg gttcgagtcc ctccacccct gccaaataaa
     1501 aaggacttga aaaaagttgg attttagagt agtataagat agtaaataag atttcgaggt
     1561 gtagcgcagt ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt
     1621 cacctcgacc aagaaaaggg cctatagctc aggtggttag agcgcacgcc tgataagcgt
     1681 gaggtcgctg gttcgagtcc agctaggccc accatttgcc cagatagctc agtcggtaga
     1741 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaggaatac
     1801 ggcggtatag cttagttggc tagagcgttc ggttcatacc cgaaaggtca taggttcgac
     1861 tcctattacc gctaccatct aactatatca ataaagaact ttgaaaatta aacagtaggt
     1921 taatttataa aacaagaaac aaaccataaa gccagatatt ttgataacaa tagtatctga
     1981 gcctgat
//
LOCUS       DAEMHZ010000251         1952 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000251 DAEMHZ010000000
VERSION     DAEMHZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.255"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..382
                     /locus_tag="KPK72_003795"
     CDS             <1..382
                     /locus_tag="KPK72_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490938.1"
                     /translation="LFIASKLLPRHMLGNVTNYGFVQETIYELKMLLPYSFEILFVEY
                     IFLLIGFVSVVFTSILINKSIYKVSTLISFLFLVIEILVFIISIEFIIPYEYSDVFTV
                     LYSSINIFVCSLISNKLLKQKIDF"
     gene            393..1661
                     /locus_tag="KPK72_003796"
     CDS             393..1661
                     /locus_tag="KPK72_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490939.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          806 a          144 c          291 g          711 t
ORIGIN      
        1 tttgtttatt gcaagtaagt tgttgcctag acatatgctt ggtaatgtca caaactatgg
       61 ttttgtacaa gaaacaatat atgagttaaa aatgttactt ccatatagct ttgaaatact
      121 atttgtagag tatatatttt tattgatagg atttgtatct gtagtattta catcaattct
      181 tataaataaa tctatttata aagtaagcac tttaataagt tttctatttt tagttataga
      241 aattttagtg tttataattt ctatagaatt tattataccc tacgaataca gtgatgtatt
      301 tactgttcta tattcaagta taaacatatt tgtatgtagt cttatatcta ataaactttt
      361 gaagcaaaag attgactttt aggagggata ttatgaaagc aaaaaatata ggaaaatata
      421 caatatgttt tataatagta tgttttttaa tgattactat gaccttaata ttgagttatt
      481 ttaataagga taaactatct gtaaaaactt taaaaggcaa tgtaaaagat atagggagag
      541 taagtgtagt atatacacct aatataagca aattttctta tgaggaggta attataagta
      601 aaaatggagt aagaaataat acaaacccaa aatcaagaat tagaaatgat tatttaaata
      661 ttgacaaaaa atacagtgac tttgttaatg ataaaggaaa agaatttata tataaagatg
      721 atttgaatat aggagaagcg tatacctctg ttaagtctga tttagtacaa ggaatgaatg
      781 ctttaaatga ttgtataaag gtttcaaata aaaatttaaa aagtggggta gaagaaaatt
      841 ttgatattaa tttaaataag tctgatttta aagaaaatag ttggaaaacc ttatttaaca
      901 aaatatataa aggtaattta tatatggtaa ttagtgaaac taatatttca ggttctaaag
      961 tcaataatat ttgtatatta aagatagata taaaaaataa gtcttataaa ttattaaata
     1021 gatttaattt aggattacag aatggagaac aacaaaatat caatgaaatt aaattttcta
     1081 ttggaagtaa attatactta gaaatagtaa ataataaaag taaaaatgga tatattgaag
     1141 gacatgatag ttactctaac tttattatat atgatataga agataacact tttaatataa
     1201 gtaataaatt tatggataat ctaaacaaag aaaagaatgc aaatccaaag gagattggct
     1261 tattaagtaa aactaagcta gattatatgg taaaaaataa taaattgaat cttatagtta
     1321 cggatatgag taattccata actaatcttg tttatgatat taattctaat gagattaaat
     1381 tagaagatta tgagatactt gatttaaaag tagattataa tgaagcatca tctggagtta
     1441 ggaaagttaa gctactggat gataagatat attctatttc aggtattaag aacagtatat
     1501 caggagaatt aaaaggattt aaagggattg ttgatgtagt cggaactaag ccagtaaaat
     1561 ttcaagtatt tgatatgaaa cttaaaaaga atatttatga agctgagcta ataaatggag
     1621 atataagttt acaagaaaag ttattttttg ttgcaaatta agtgttagaa ttaattttag
     1681 aactttgaac ttttatatgt atcacagata agtataagta agggagtgtc tcaaagtaaa
     1741 ctttttagct catgagacac tttttttata tgaaatcaaa tatattttca ttgtttagat
     1801 ttattttgat aatgaaaaaa agattatctt tattttaaga aagacattta tttataatta
     1861 tatacaaaaa tattttttaa tgtttttgtg tataattata acaatttaat ttatattaat
     1921 gcacttaaat ttgaaataga atgcttatag tg
//
LOCUS       DAEMHZ010000252         1864 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000252 DAEMHZ010000000
VERSION     DAEMHZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.256"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(284..1612)
                     /locus_tag="KPK72_003797"
     CDS             complement(284..1612)
                     /locus_tag="KPK72_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2490940.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          681 a          333 c          212 g          638 t
ORIGIN      
        1 ataaaagcta tgtataatat ttgttgtaca gataccactt agtttgataa aaaacacttt
       61 aaaataaagt attttaaaat aaataatttt ttaggtgttt aattttataa tcaaaaaact
      121 aaaaataaaa tgaatacctt aaaataaact ttttagttca tggaacgctc tttttttgta
      181 aaatcaaaca tatctttacc attttaacaa taaaaaagag acttatctct atttgagaca
      241 gtctctttta ttttaattat gaagcatttg taaactactt ggtttatctt tttactcctc
      301 tagtcatata agcatacact ggtaagccta tagctgttat tacaagacct cctatagata
      361 tcatagtagt tttcatacct gcaaaacaca attgattaac tacaacaaat ataccacttg
      421 ctatagctat tataggaaca ataggatata atggaacttt atactctctt ggtatatttg
      481 gatgagtctt tctaagtttc ataaccccta taaaagttaa tacatagaat atccaagtag
      541 caaatacagc taagtcagtt aataaattaa attgacctga caaagcatat atagatgcaa
      601 taacagatac taatgctatt gcatttgctg gaacttgagc agaatttaat ttagaaaata
      661 taccatactt tggtaaagtc ttatctgtag ctaaagtata tgccaccctt gaaccagtta
      721 gtaaaaatcc atttaatgct ccaaacactg aaattaatat acctacagat attatttttc
      781 cacccattga accaaatata acttcagcaa ctgctgatgc aggagaagca taatttgcta
      841 attcattagc tggtaatacc caaagatacg ctaaatttat tataaaataa acagccataa
      901 caactgacaa tccacctatt atagcttttg gaaggtcttt tccaggattc ttcatttctc
      961 cagctaaagt accaacattc atccatccat caaaagcaaa taatatagca attaaaactt
     1021 gacctagtac actacccagg cttaatcctt caccaaccat aggtgtaagt attggattat
     1081 ttccaccacc tttaataaat ccaacaacca ttattaaaat aagtggtatt aacttacaaa
     1141 ttgtagaaac agtttgtatg actcctccag tttttgaacc taacatatta agccctgcaa
     1201 ctattactat aattccaatt gctattggaa gtaaaagtga tggactacca attagtgctg
     1261 atgcttgttc tccaaatata acacctaatg ctgccatcat ccctggataa aataatacaa
     1321 tttgcatcca tccagttaaa aaacctaatt tttctccata aatttctttt atatatacca
     1381 tcattccacc agtttttggt atcgcaactg atacctctgc agctgtaaga ccagctgtta
     1441 tagttatgat tccagctatt aaccatgcca atataccaag acctggagct cctccagtta
     1501 atgtatagac tgcttgtggt ttaaaaaata cgcccgctcc tataacggaa ccaacaacag
     1561 ttgataatgc tgctgatacg ccaagagtct ttttcagttc attacttccc atttttttcc
     1621 tcctcattaa tcctatttaa ttttaatttc atagttaaat aagcatatat tatgccaata
     1681 tatagaaaaa tagctttatt atttattaaa ataaaataaa ctctacttag ctttagtaat
     1741 cttttatttt tagttatgtt taaatataaa aaaattataa aaaccaaaaa tccaaatata
     1801 ataatacttc taagtaaatg tattttttat attgtataat atacattgta tatttgtaac
     1861 acta
//
LOCUS       DAEMHZ010000253         1860 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000253 DAEMHZ010000000
VERSION     DAEMHZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.257"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            282..>1860
                     /locus_tag="KPK72_003798"
     CDS             282..>1860
                     /locus_tag="KPK72_003798"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG2490941.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTT"
BASE COUNT          704 a          217 c          285 g          654 t
ORIGIN      
        1 tatttataaa tttttagttt attgacgtat taattaattt tttcccaggt agatttaatc
       61 aatttatttt aaataaaagt aaatttaatt ctatgtttgg ttgggtaaaa gtatatataa
      121 taataatttt tatattaaat ttgtagtatt tatgtaagaa ataaatttta tataatattt
      181 tttcactaaa tttttttctt tttacaacaa atactcacta ccttttgata taatattttt
      241 atcaattatt attatattca agttttgaaa ggagtattaa aatgagttta ttagttttcg
      301 gacataaaaa tccagataca gattcaatat gttctgcaat ttcattagca catttaaaga
      361 acaaactagg aataaaagca acagcttatg ctttaggaga tatcagaaaa gaagctaaat
      421 atgccttgga ttactttaag gtagatgcac ctgaaatttt agataatgtt agaatacaag
      481 ttagagattt aaattatgat aaagtagtac ctttaacacc aacttcatct atattagagg
      541 cttataacct tatggatgaa aaaaatgtta aaacactacc tgtaacttat gatgatggaa
      601 cttttgctgg tatcattact atgaaagaaa tagctaaaaa tctacttcat caacacttta
      661 caactataca cacatcacta tcaaacattt gtaaaaacct taatggtact attcttgtgg
      721 actgtggtga agatataaaa ggtagagtag tcactttatc atttggaatg gagactctaa
      781 tagagacttt aaaagaagga gacatagcta ttgttggaga tagatatgat agtattgaat
      841 atgctattga tacaaaagtt aaacttttaa tacttactaa tcatacagag atttcaaaag
      901 atttgctagc tcttgctaag ataaatggag tgtctgttgt ttctgttgaa agtgacaatt
      961 ataaaacttc taatgtaata aatcaatgta gtttcttaga ctctatagaa gctacagaaa
     1021 atttaattac attcaaagaa gatgattata tggatgaaat aaaagagatt atgctagaaa
     1081 caaacttcag atcttatcca atattagatg atgataataa gtttttaggt ttagtttcaa
     1141 aaggtcatct attaaaccca tctaaaaaga atgttgtttt agttgaccat aacgaatatg
     1201 ctcaaagtgc tgatggaatt gaacaagcaa acatagttga aataattgac catcataaac
     1261 ttggtggtat ttctactgat gttcctatgt catttagagt tatgcctgtt ggatgcaata
     1321 gtactataat ttatcaaatg tacaaagaaa ataatgttga gataccttat gaaatagccg
     1381 ggcttctttt atctgctata ttatcagata cattattatt taagtctcca acaacaactg
     1441 atatagataa gaaagcttgt gaagaattaa gtaaaatagc aaaagtagac atggaaaaat
     1501 acgctatgga tatgtttaaa tgcggtactt ctttagatga atatactata gaagaaatag
     1561 taaatatgga ctttaaagaa tttaatatga gtggacatag agttggtatt ggacaagtat
     1621 ttaccttaga catagactct atatttgcta aaaaagatga gtttttatct tatataaatt
     1681 cgactgatta tgataaatta attctttcag ttacagatat aatcaaagaa ggttcttatt
     1741 taatctataa agcagaagat agcttaattg ctaacgcatt tggtgttgaa ggtgttcaag
     1801 gcacatttgc accaggtgtt gtatcaagaa aaaaacaatt agtccctaat ttaactactg
//
LOCUS       DAEMHZ010000254         1837 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000254 DAEMHZ010000000
VERSION     DAEMHZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.258"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..842)
                     /locus_tag="KPK72_003799"
     CDS             complement(<1..842)
                     /locus_tag="KPK72_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2490942.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIN"
     gene            complement(836..1546)
                     /locus_tag="KPK72_003800"
     CDS             complement(836..1546)
                     /locus_tag="KPK72_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490943.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
BASE COUNT          644 a          250 c          145 g          798 t
ORIGIN      
        1 ttatttatat atgatataat ttcttcttta gtgtcataat tttttataca aatcatatgg
       61 tttttcaagt catgataaac atattttaat ctatcatgaa cttcgtttat attttcataa
      121 ttcctgtagt ttgtttttat tctttcattt ataagttcat actctagatt taatttatcg
      181 tcttgtacaa ttttccctat tactattaaa agtattatac ttgatgataa aatcaataaa
      241 attgcaaata taaatataaa taagttttct gtagtaattg tattgactct taaattatat
      301 tcaaatataa gtaataagct aactatatta gataatatag gtattcctat tagtatcata
      361 tctttaggtt taaaatctaa agataattta aaatacttaa acaatataaa acctataaat
      421 aaaaatacct ttgacataat aatagcttgt attctaaata agttttcatg ttgtgttatg
      481 ttaatattat ttaatttatt tataaatact actagaccta ctgcagaacc ttcagcaacc
      541 attaatccta gccaaaacaa taaactaact attatacttt tatatatttt atcttcatat
      601 gatgttatat aaaatgatat tcctattacc atacatagaa gaatcttaat atttggaaac
      661 actttaacta catttatgta tgccataaat ataaatataa ttattgttgt aaatatatat
      721 cttatattag atattttcct ctttccaact aagtggatta ctaaaaaaaa tataatccaa
      781 tctatagtac ctgaaactaa attaataata ttccaaaatc ctacacttaa atttgttagc
      841 atactatgtc ccctaatata ttagttatcg caagcttcaa gcctttaacc ctatatttac
      901 ttattggaat actttttcca tctactataa cagtacttcc actcattgat tctactaatt
      961 ttaagttaac tatatagcta ttatgacatc taaaaaatcc atattcattt aatatctttt
     1021 ctattttaga tatactcatt ttagtaatat acatatcatc atgtgtataa atcaatatat
     1081 tcggtctatc tgtttctata taagtaatag aatctatttt aattcggtct acataatttt
     1141 ttacatttat agttaagtaa ttatttctct ttttcatcat ctcatttata caaggtaata
     1201 cacttttaga aattttttct ttatttattg gctttagtat atatctgtat gcttttacct
     1261 cataaccttc ttgcataaac tctacaaatg aagtaacaaa tattatttct aacttatgat
     1321 caaactctct tatcttccta gcagtatcca taccatttat tgttttcatt tgtatgtcca
     1381 taattaatat gtctaaatct ttaggatagt tatttagtaa ctcttctcct gctgaaaatt
     1441 catatatgtt ataatttata ggatatgaac ttaatatttc acttaatata tcttttattt
     1501 ttattcgata atgtagttca tcatcacata ttcctatatt aatcacaatt cttaaacatc
     1561 ctttcttata tatttttaat aaaaatatat aaatttctat tcattttaca tattttaaca
     1621 tacttctgta gtaaatagct atttagattt taattttagc tttttggaca ttaagtatta
     1681 ctttttggat ttgcaatcta ttttgatttt cattataata caatttttta acatatatta
     1741 aatatattta gggattttaa taaaatattc ctcgaaaagt ttttaatatc tagtttttgt
     1801 taaataaaaa gtatgcacct tcctttttat agtaatg
//
LOCUS       DAEMHZ010000255         1836 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000255 DAEMHZ010000000
VERSION     DAEMHZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.259"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(417..806)
                     /locus_tag="KPK72_003801"
     CDS             complement(417..806)
                     /locus_tag="KPK72_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2490944.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          599 a          322 c          106 g          809 t
ORIGIN      
        1 gttaacactt ttatctctat ctctttaata tcttatatta atcctttatt tttatttttt
       61 caatatcttc attaattcct atttatttta tcatcttaaa tacgaaaaca ctgtgtcata
      121 tttacaaaat atatattatc atctatatta aagcttcaca atttatattt aaaaattctc
      181 tttaattaca ctacctctat ttaaaaagct accttaatta tacttataag taaaggtagc
      241 tttttaaatt taattaaata gaatatccac tattaatttt tatttaattt atttagattc
      301 acattatagt atcataatcc acttataacc atttgtcata ttacttaagt agactaatat
      361 attacataaa tacacttaca tacactttat attgatgcta taagaaatac gtttgcttat
      421 acgtctttct cctcttcttt tagatttttt atccactctt caataagtaa gatatcatct
      481 ttgctcaaat taatattttc atgtaatgac aacaataaac tttttataga agaatcatga
      541 atatttctaa aaaaatctct tgtctcaact cctatgtatt ccttttcttt aattaaaact
      601 tcataatgcg tatatttatc tatcttttga gaatttagaa atctttttac tactaatcta
      661 gataaaagag tcaaagttgt cgtttgtttc cattgatatt tttgctccat tcctaaaaca
      721 atatctctag atgttacttt ggaatctgat ttccatataa atttcattat ctttaattct
      781 gattgtggca acttttttat agtcatgata aaaccttgct actcagaata tagacaagca
      841 atatccataa taaaattttt cgattaaata catcttatac ggatttatat aagctcagac
      901 ttataagata tttttcaatc ttaatcctaa atttagctat ttgtacacgg taaatatata
      961 taaataatgt caaaattctc ttgtattata ttgcttaatt ttatgaaata ttttgtactc
     1021 agctatctat tttggatact tgtccagtag ctctcctttc ttttaaactc tttgatttta
     1081 aatatttgta tttcagtaaa tcctacttcc taaatttttt atttactaca acttattact
     1141 gaacttccta cttcctaaac tttttattta ctacaactta ttactgaact tctcacttac
     1201 taaatttctt acttactata aattataact aaacttctca cttactaaat ttcttactta
     1261 ctataaatta ctactaaact tcctactttc taaatttctt acttgctata atttattact
     1321 aaacttccta ctttctaaat ttcttacttg ctataaatta ttactaaact tcctactttc
     1381 taaatttctt acttactata aattattact aaatttccta ctttctaaat ttcttactta
     1441 ctataaatta ttactaaact tcctactccc taaagttctt acttgctata aattattact
     1501 aaacttccta ctttctaaat ttcttgctta ctagcactta tcactaaact tcctactttc
     1561 taaatttctt acttactacg acttattact aaacttctca attactaaat ttcttacttg
     1621 ctataattta ttactagact tcctactttc taaatttctt gcttactaac acttatcact
     1681 aaacttccca cttactaaat ttcttgctta ctaacactta tcactaaact tcctactttc
     1741 taaatttctt acttgctatg aattattact aaacttttta cttcttaaat ttcttacttc
     1801 ttaagaataa tgttaatgtt aattttaaga ttatag
//
LOCUS       DAEMHZ010000256         1784 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000256 DAEMHZ010000000
VERSION     DAEMHZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.260"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(188..1101)
                     /locus_tag="KPK72_003802"
                     /pseudo
     CDS             complement(188..1101)
                     /locus_tag="KPK72_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            1357..1713
                     /locus_tag="KPK72_003803"
     CDS             1357..1713
                     /locus_tag="KPK72_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490945.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT          640 a          211 c          238 g          695 t
ORIGIN      
        1 ttttaacaat aatatataat tatgatttat ctaattataa gatatagtaa ctatctatat
       61 tattatataa tataatgata aatatatatt aacaaataat aaaaaaatta atttgattgt
      121 atataaatta gtttgttatt aaattagtat taaaaagttc aaaaatttac ttagaaggaa
      181 tctataatta gatttctatg tttgttgaga atgcaattac agcttttcca attattttta
      241 cttcaactgg cttattatct aaaataatta cttttactaa catcttgcct ttgcgtttta
      301 ttgcttctcc ttgaactgct tcaaatataa actctgtgtt gggtgtaggt attactttat
      361 aatgtacaag gtacgcacct aagggtccat tagcatttcc tgtaacaggg tcttctgaaa
      421 taccaattgc aggagcaaac attcttccat gaacttgtag agtttcttta ttatgtaatg
      481 taaaaatata atgtccatta cattttattt cttgacttat tttcgataat tcatataaat
      541 ttggagataa actatttaat gttgataatt cctttattcc aatcataact tttgaatgtc
      601 ctgtagatga tatggcaata ggacaatctg tacgaatatc ttcctttaaa ataccaaatg
      661 cattaagtaa tcttttttga taggtttgtt gtaatggtgg agatacttca atttttcctt
      721 gtgtcataat tatttcataa tcattatctt tttttataat atcaactggt aatattccag
      781 caccagtttt ttgaaatact gttttgttat tgaattggtt ttctattgct ctaacataat
      841 gggcagcaat agtagcatga ccacatatag gaacctcatt agttggtgta aaaaatctga
      901 tatgtacatc ataattttct tgatgatttt tagccaagtt attgaaaata aaagcagttt
      961 ctgaattatt taattctctt gctatttttt gcatttgtga ttcagataag ccatcagcat
     1021 ttgtaattac accagctgga ttacctgtaa atttttcttt tgtaatgatt caacttgata
     1081 aagacgaaat tttttattca tattatataa tcctttctaa acattagatt agtttatcta
     1141 catagaaaac ttgagataaa ttattattta ttattatctg tttaaataaa attaataaaa
     1201 taacatatgt ctatattaaa atttaaacat atacatctaa aaaagtagta ttatgtaagg
     1261 ctctatacta caatcaagat tatattacga attagtacgc taaacaagaa tgcacttttt
     1321 agttagatac ttactatttt gaaagagagg attgagatgg atatagataa taattttgaa
     1381 gaatttccaa atattgaaga aagacaaaaa gattttaatt gttcaattgg ttttaccatg
     1441 actgtgatag gaagtaaatg gagggcaatt attttatggc atattttaaa acaagagcca
     1501 atacgttaca gtcaattaaa aaaagaagtt gttcatataa gtcataagat actgtcacaa
     1561 gaattaaagt atcttgaaag agatggactt attaagagga tttcttatcc gactattcct
     1621 ccaaaagttg aatatttgtc tactaaacgt ggtaaatcat tagaatctat attattagaa
     1681 ttatgtaatt ggggtaaaca gtatatgaga taaactcaac tgtttacttt atgaaaaata
     1741 tataatgaag cttattttaa ttagttcttt aatgaattat aaaa
//
LOCUS       DAEMHZ010000257         1782 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000257 DAEMHZ010000000
VERSION     DAEMHZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.261"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            224..1099
                     /locus_tag="KPK72_003804"
                     /pseudo
     CDS             224..1099
                     /locus_tag="KPK72_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT          682 a          237 c          335 g          528 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atagtttaac agtaggaaat atcgtatgag
      121 taagactatc gtattttaca taaagattta gcaatatgac acactgttgt cgtgttctgt
      181 aggatatgat taataaatca gatatgaaag gagagtgagt aatatgccag aattactaaa
      241 aaatatgtat aatcgtgagt cactttatga agttgcagta gctatccaat cagtttataa
      301 ctcatttaag gtagacgagt tcataaaatc tacaatggat gagacatgga ataacttaga
      361 attaaaagcc agatgtcgaa agataagtat gagtttagga atgtatttac cagaagacta
      421 taaggaagct ctttccattc ttgaaaaatc agttacaggt ttttattttg cttttttctt
      481 tccagatttt gttgaagttt atgggcagga cgacataaat tgggatttat ctataagtgc
      541 attagaaaga aatactgagt attggtcttc agaatttgct gtaagagcat ttatcatcaa
      601 ggatgaagag cgtatgatgg cacagatgcg taaatggtct aagcataaga gcgaacatgt
      661 acgaagactt gcaagtgaag gttgtcgtcc ccagttgcct tggggacaag caatatccaa
      721 atttaaaaaa gaccctacac ccgtactgcc tatattggag caacttaaaa cagatacatc
      781 aacgtatgta caaaagagtg ttgctaataa tctaaatgat atctcaaaaa cacatccaga
      841 tttagtcata agcattgcaa aggattggta cggtaaaaat aaatccacta actggattgt
      901 aaagcatggt tgcagaactc tactaaaaaa aggcaatcgt gatgtattag ccctttttgg
      961 atatgatgat actacttcta taaatcttca ggactttact ttagaaacaa cgtctatttc
     1021 tattggagag gatttaactt tttcatttaa gattttagca aaaaaagcga caaaaacaag
     1081 gctcgagtac ggaattgatt aaaaaatagc tacgcacact cgtgacttta gtcatgagtt
     1141 agtagctaaa ttagtcagca tatagggaaa cttatatgta gaggtaggat agaacctatc
     1201 caatactact tgaattgctg gaaatcccta aagctaacca aactaaaaca taaggatgaa
     1261 ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt ataaggttaa
     1321 accctaagta ctttaaaatg ggcaatcagc aggtaagctc cgaataggag aaacttcaac
     1381 gactattcct cttgagggaa gtacactaca agctattggt agtggaagtg ggtagacctt
     1441 aacggataat gccgaaggat aagatatagt ctgtgcttac gtgaaagtgt aagaagttca
     1501 taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa aatgataaaa
     1561 gttaagactt acggttctta aatatgttat ttaaaattaa atataaaacc ttctaaataa
     1621 ttgttttaaa taattacatt tgttaacatt attataggtg attaataaat tgttcaccac
     1681 agcattatat aatatattaa atgtgataaa atcattaaaa agtgtatcaa aaaacaatta
     1741 aagtgaggtg atagcagtga aaaaagctta taagtttaga at
//
LOCUS       DAEMHZ010000258         1749 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000258 DAEMHZ010000000
VERSION     DAEMHZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.262"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            287..625
                     /locus_tag="KPK72_003805"
     CDS             287..625
                     /locus_tag="KPK72_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490946.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            647..964
                     /locus_tag="KPK72_003806"
     CDS             647..964
                     /locus_tag="KPK72_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490947.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1114..>1749
                     /locus_tag="KPK72_003807"
     CDS             1114..>1749
                     /locus_tag="KPK72_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490948.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYT"
BASE COUNT          765 a          169 c          257 g          558 t
ORIGIN      
        1 atatattcta catatctaat atgtaattac aataaaaaat gctataatta acatattatt
       61 aattcgtttt attattgtag tatggctatg aaattatgaa aatatttctc taattaaaaa
      121 tatgatttat ccatatttgt ttattgtaga tacagactat acaaaaataa atagcactta
      181 tttcagtata aaaacatata tagtctatat ttagactaaa aaagtataaa tagtctatat
      241 aaaacaactt aaactatgaa tataatctaa agtggaggga taataattgg caaatatagg
      301 aaaaataata ggagatagaa taaaagtcta tagaacaaaa ttgggatata gtcaagagtt
      361 tctagcagaa aaggcaggtt tacatccaac atatattggt cagattgaaa gagctgagac
      421 aaatacatct atcaatatta taatgaaaat tgctactgct ttagacatac cacttgaatt
      481 gttatttgca aatattatta caacagaagg cgttgataac tctgtacctt tagaatgtta
      541 tgaaatgatt aataaattaa ctgaaaaaga acaaattgca atgctagagt taataaagtg
      601 catcattaaa tacaaagaaa tataagtgag ggaaatagta acaataatga attatatagg
      661 taaaagactt aaagaagaac ggaaaaaagc aaatttaact tcaaaagaat ttgcaaatat
      721 ggttggagta tcgccttggt atataactca aatcgaaagt ggtaaaaaga atccaagtct
      781 taaaacattc ataaaatttg taaatatatt aaatatttct gcagatgttc ttataaaaga
      841 tattacttcc actggaaaaa catatttaga aaatgatata aatgaagagt taaaagactt
      901 aaattcaaga gaactaaatc tgatatcaca gattatagac atcataaaaa agaataaaat
      961 ataattcttt atattttata taaaaaaaaa ggttatttga aataaataac ctttttttgt
     1021 ctttttgtgt taacattgtc ttatagtgct aatgagtact attagaccat gttctatggc
     1081 cttattttaa tataaaacga ttggagatag gctatgattg tgattgaagg tagcgataaa
     1141 tttaagattg caaaagaata tattgatgta gaatatactc tttttagcaa agtaactttt
     1201 aggtatgaaa agttgaaatt taaagataat gctgaattgg aaaaaattaa gatgtttaaa
     1261 tataaaaatg gctacatccc taataaatta aacctttctt ttggatatgg attctcttct
     1321 tataaaaagc aaataattag agaaactgta gatactttaa gattgacaga aattttttca
     1381 agcgagaaca tagaagatat taaatttata aaagatggta caaaaaaatt agaaattagc
     1441 atagagaaag ttgtgaaatt taaacgtcga aaaaaaagaa attatgtttg ttgctattgc
     1501 cctgatatgt atagagacat aaaactcgac aaagaatcta tcaataagat atacaacaga
     1561 aaaataaaaa tagaaagaga agttaatatt tttgaagatg aggatgttat aataaacaaa
     1621 aaagtattga agtttccaaa gtcttggaca aagaatatgc aaaaatattg gttaagtgaa
     1681 aataagtatc ccatacattc tactgtaatt gatgatgata gatataaatg ttgtaatgta
     1741 caatataca
//
LOCUS       DAEMHZ010000259         1717 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000259 DAEMHZ010000000
VERSION     DAEMHZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.263"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            64..762
                     /locus_tag="KPK72_003808"
     CDS             64..762
                     /locus_tag="KPK72_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2490949.1"
                     /translation="MEKKYFEIHGVPSIIWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWVNVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          678 a          200 c          195 g          644 t
ORIGIN      
        1 acattatttt atagtttgat taatttaaca atcactaata tatcaaactg aaaggagatt
       61 tttatggaaa aaaaatattt cgaaattcat ggagttcctt caatcatttg gggaaatcct
      121 tcaagtaaat tatatattta catacatgga caatgtggca ataaagagga ggctgaagct
      181 tttgctaata ttgcagttcc acatgagtgt caagttttaa gtgttgattt gcctgaacat
      241 ggagaacgta aaagagaaaa aaattcattt aacccatggc atgttgttcc agaattagta
      301 tctattatgg aatatgcaaa gtgccactgg aatcaaattt cgttatatgc aaatagcatt
      361 ggagcatggt ttagtatgtt aagctttgaa aaagagaact taagagagtg tatttttgta
      421 tctccaattc tagatatgca acagctcatt tcaaatatga tgctttgggt aaatgtatca
      481 gaagaacagc ttaaacatga actaattatc ccaacttcat ttgggcatac tctttcatgg
      541 gaatatctac aatatgtaaa acaacatcct atcactcaat ggaatgtacc aacaaaaata
      601 ttatatggta aacatgatga acttactgaa atcggtgttg tagagaaatt tgtagaaaag
      661 tttaattgca accttactat tatagaaaat ggcaaacatt ggtttaacac accacaagaa
      721 ttaaattcat tatatgaatg gttaaatact ttagttaaat aaaaaataat tatattatta
      781 atatgattaa attatttata aatgttattt aattaatttt attttagcat tattaaaata
      841 actacataaa gaataaatta gttattatta aagaaattaa aagtatatag agtattaata
      901 attttttaat aattagttat tatagaaaaa agagggatat aatattgtct ccctctcttt
      961 cttctgtaat attatataat ttttatttat cactctcaat aaattatagt tatctacaaa
     1021 attcaatctt taatacacta ttaatatatt gtgatattaa tattacagta ttaaataaaa
     1081 tctaataaaa ttaactataa aatatctatt atataaatat ccactcttat gtttgaaatc
     1141 tgtcatatag ttttatcctt cttatctttt atatcaataa ctttttttaa taaaattgac
     1201 aaaactaaag taaaaaacct tattgataat gaaacaaatg atactataag ttctgatatt
     1261 atagtaattg catataaata gacatcataa aaataatata atattgtcac tataaaatta
     1321 aatgcaaaaa tctcaaatct aactaaatac acatataggt attttttaag tttttaggta
     1381 tttcttctga aaatgattta ttgattgata ccaatttaca taatcttttt aatataaaca
     1441 atgatacaat atatgggaca gcacatatgt atatagctac ttatattaca tattacagtt
     1501 ggcatattcg tatttgtaat gcttatacca attttaaaat acaccatcag tactaatggt
     1561 attaaaataa tctataataa agttaaaatt attccaatta gtacaatatt atttaatatt
     1621 ttacaactca tattcttttt tatataaatt taccacctta tacattctta tttttagtgt
     1681 atattacaat ggttttatat attatatcaa taattta
//
LOCUS       DAEMHZ010000260         1684 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000260 DAEMHZ010000000
VERSION     DAEMHZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.264"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            348..755
                     /locus_tag="KPK72_003809"
     CDS             348..755
                     /locus_tag="KPK72_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490950.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            1394..>1684
                     /locus_tag="KPK72_003810"
     CDS             1394..>1684
                     /locus_tag="KPK72_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2490951.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKA"
BASE COUNT          690 a          176 c          282 g          536 t
ORIGIN      
        1 tatttaataa agtacataat agtttagtaa aatttctcaa tatggacaaa atatgttaga
       61 atatagttta aaaagataat aaaatctgtt ttttactttt aaagttatcg ggtagcaact
      121 ccatttcttt gaatataatt gaagctatac aatttatata ggaaaaatgt aaaacttatt
      181 ggagaaataa aaaaatatga agaataatgt agtttgtgat atattaagaa ataggtgtta
      241 attatatttt gatagggcta agcaaggcaa attagttgat agaattatat ctaatgagct
      301 aaattttact tgattaaaaa gaggtatgtt agaagtggag tgataaaatg agcaaggtag
      361 ctaaagcaac tttttatttg atgatagtta ctataatctc taagattctt ggaatgggta
      421 gagaattagt attatcatca atttatggta caggcttata tacagagagt tatttaacag
      481 ctatgaatat accaaatatt atatttgcag caataggtac tgctatagtt actactttta
      541 ttccaatgta tcaagatata agtagtaaac aaggtgaaaa gcaagcacta aagtttttaa
      601 acaatgtttt aaatataata gttggtatat gcatagttgt agctatatta ggagttattt
      661 tctcaaagca acttgtaagt atatttgcta taggatttga aggagagagg tttttactta
      721 ccgtaaaatt tactaaaata ttgattaaaa aatagctacg cacactcgtg actttagtca
      781 tgagttagta gctaaaatag tcagcatata gggaaaccta tatgtagaga taggatagaa
      841 cctatccaat actacttgaa ttgctggaaa cccctaaagc taatcaaact aaaacataag
      901 gatgaaataa acctaagtgt gaaagttacg aaagtagaaa aaattggtta gatggtataa
      961 ggttaaatcc taagtacttt aaaatgggca atcagcaggt aaactccgaa taggagaaac
     1021 ttcaacgact attcctcttg agggaagtac actacaagct attggtagtg gaagtgggta
     1081 gaccttaacg gataatgctg aaggataaga tatagtctgt gcttacatga aagtgtaaga
     1141 agttcataag agaactgcat aggtagtagc gaacttatgt gaacgacaac ctcaaaaatg
     1201 ataaaagtta agacttacgg ttcttaaata tgttatttaa aattaaatat aaaaccttct
     1261 aaataattgt tttaaataat tacatttgtt aatattatga taggtgatta ataaattgtt
     1321 caccacagca ttatataata tattaaatat gataaaatca ttaaaaagtg tatcaaaaaa
     1381 caattaaagt gaggtgatag cagtgaaaaa agcttataag tttagaatgt atccaaataa
     1441 aaaacaacaa gagttaatta acaaaacttt tggatgttgt agatttgtat ataataagta
     1501 tcttgctaaa agaatagatg tttataaaaa cgataaagaa acttttactt ataaacagtg
     1561 tagttctgat ttgaccaatt taaaaaaaga attaaaatgg cttaaagaac ctgacaaatt
     1621 ttcactacaa aacgccttaa aagatttaga taatgcatat aaaaaattct ttaaagaaaa
     1681 agca
//
LOCUS       DAEMHZ010000261         1642 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000261 DAEMHZ010000000
VERSION     DAEMHZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.265"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            442..>1642
                     /locus_tag="KPK72_003811"
     CDS             442..>1642
                     /locus_tag="KPK72_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2490952.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIIS"
BASE COUNT          609 a          210 c          217 g          606 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttctttt tccttttttc
       61 aaggacaagt aatactatat catctttatc tattctagtc aatataattt tttacttttt
      121 ttaatttttt tatttatcat gtattcttta tctttaattt ttaaaactta atgctaagta
      181 aacctgttta tttcataaaa ctaatatata ttctatgctc tattttcctt tttattctat
      241 atttaaattt tttatttaca agaatatttt taatataaca tatttgataa aattaattta
      301 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
      361 caataacctt attcacaaag acagttttta tatatcaaaa tataatataa atactatgct
      421 acattttagg aggattttat tatgagcaaa aaagtattaa taatgtctgc ttccactggt
      481 ggtgggcata atagggctgc tctagctatt aaagaagaac ttacttccaa aacacttgat
      541 ggagagccaa ttgaatgtga aataatagat agtttgaaat tagttaataa tacaatggat
      601 aaaataattt caagaggata cgaaaaatct gctatatata ctcccaaagc ctatggaagt
      661 gtatatagat tatctgaaac aaatcttttg tcaaaaaatg aatttaaaga taatctacta
      721 ataacattca tggctaaaaa atttaaaaag cttattagaa gcgaaaaacc agacttgatt
      781 ataggaacgc atccttttcc aatgattgct ctaagtacat tgaaaaaaaa ctttaattta
      841 cataataatg aatcaaatgc ttacacagag catttttata aacattatac aaataccatt
      901 aatgttcctc cgcttatttc tgtattaact gattatacta ctcattctac atggatacaa
      961 aatgaaattg attactatat tgttggtcat gaatatgtaa aagaattatt ggtgtttgat
     1021 ggagttgaac cgagtaaaat tagaacattt ggaatccctg ttgaaaaatc ttttctctct
     1081 cacagagata aagatattgt tctttctgag ttgaatttat ctcctgataa attaactgtt
     1141 ttacttatgg ggggtagctt tggggctgga aatataaaag agacacttga tgaattactg
     1201 gatactgata gagactttca aatacttgta attactggta agaatgaaag cctaaaagaa
     1261 aaaatcgaaa aaaaacttat gtctcgttat catgataaaa atgtatgtgt tcttggatac
     1321 actgataaga tgaatgatat tttagcttct atagatgtat tagtaagcaa acctggtgga
     1381 cttactacaa ctgaggctct tttaaaagat gtacctatga tagttcctta ttatatacca
     1441 ggtcaggaag aagagaatct tgatttctta tctaattgtg gtgcagcact tagaactaca
     1501 aaaaaatata acttgtcaat attactaaaa gttttaatag atgaccctag cagattagaa
     1561 atgctaaaaa gaaatattaa atcaataaga aagtcaaatt cagcccaaaa tatagctaat
     1621 ttaattttaa atataatttc tg
//
LOCUS       DAEMHZ010000262         1620 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000262 DAEMHZ010000000
VERSION     DAEMHZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.266"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..860)
                     /locus_tag="KPK72_003812"
     CDS             complement(<1..860)
                     /locus_tag="KPK72_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490953.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKI"
     gene            complement(865..1602)
                     /gene="cas6"
                     /locus_tag="KPK72_003813"
     CDS             complement(865..1602)
                     /gene="cas6"
                     /locus_tag="KPK72_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2490954.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
BASE COUNT          505 a          273 c          124 g          718 t
ORIGIN      
        1 cttatttttt catgcaaaag ttttttataa aatgatttat ctgaatacat ttttctttta
       61 agcattatct tatgatttaa aaatgctaat tctcttaaat cattcgtata tgcaaaaacc
      121 attggtcttt ttaaatttat atataaaaga cacattaagt tgaaaaattc acactcaaaa
      181 caaatattac tttcaacttg tccacttctt ttaaaacctg atttttctgc acctctaaat
      241 aaaaagttat acttagagtc ctttcgttct cttaaatcaa tatctagttt tgttgtttta
      301 tttatattgc aaacattaca tacactatct ttttcttcat attgtttcaa atcaagttta
      361 tctatttcat ctatctctat atttgattta atcacaatat cgactattct ttttacattt
      421 gtttccatat tttctagact cgtgtttaat ccatacttac cactatttct aacatatgga
      481 aatcttctgt ttttaaaatc ttttttaatc ccttcctttt gtttctcagt tgcagaagat
      541 ttatccaatt cttcaataaa atttacatat gttaaattat ctatattact aaatattttt
      601 tctaaggttt cctttttccc atttttaaat accttaaaaa gttgattttc taatatataa
      661 tcagatatat acttagggtc tatatattct gcagattcta aagataaata atttctattc
      721 aactcacaag taaattttag cttataatca tttttttcta gttcgttttt taaatccatt
      781 agtaattcac aaaagtaaac aatgccaata tctcttacaa tatcacctga tctataaaat
      841 ttagctttta tttctctcac ctcgctactt taatatatca accattcctg cattttccgt
      901 ccttctatat ccaattccca ttttatacag tgcatttaaa tcttccatat ttccttttaa
      961 aaaaaatgtt cctgcataag catttatata gtaaaattct ctctctttaa atccacgaat
     1021 cttttctttt aacactcttt ttttgaagtt aagaggtata aattccaatg gctttctaag
     1081 cccactacct cttatactct ccagagttag attagctata tagtttaaac attctatata
     1141 atttgaatct tctacatcta aatatttacc ttctttattc tttactatta taggagacat
     1201 agttttaaat ataacaccat tttcttttat ctcgtgttct ttatgtattt ttatattttt
     1261 tattttaaat ctataacctt tatattctag ctcctttact tttaaaattc cattgtataa
     1321 tttactgaac atagaataat tataaaaact tatattgaat attatatctc catcaacaag
     1381 taactcattt ttgcttctta taagatttgg tatgtaaatg gctgtattta tatcctttat
     1441 ttgcttattg ttttttcttt cttccccata gttaaatatg tttttagcaa aatctttgtt
     1501 ttctgcattt aaaatatttt ttataaagga aactgacaac attgaatttt catttggaaa
     1561 tttgccatta catgattcca taaaaattga aattctgctc atattttcac ctcctacttt
//
LOCUS       DAEMHZ010000263         1606 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000263 DAEMHZ010000000
VERSION     DAEMHZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.267"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..293
                     /locus_tag="KPK72_003814"
     CDS             <1..293
                     /locus_tag="KPK72_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2490955.1"
                     /translation="GVTSTTINISAEITYKNSYTWENIKSIAEEAVDDYLNELNMSWE
                     DEENLIVRISQIETRLLSINGVLDITNTMINDVKSNLTIDSNSIVARGEVVG"
     gene            286..903
                     /locus_tag="KPK72_003815"
     CDS             286..903
                     /locus_tag="KPK72_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG2490956.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNAENKEIKILHDKLNDLTS
                     DQFLEDLTPFGIKRWEKIMSITPKSNETLDDRRFRIFSRYISKLPYSERFLRNWLDNI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNH
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEVTIF"
     gene            915..1463
                     /locus_tag="KPK72_003816"
                     /pseudo
     CDS             915..1463
                     /locus_tag="KPK72_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          685 a          188 c          291 g          442 t
ORIGIN      
        1 aaggagttac aagtacaact ataaatatat cagcagagat aacatataaa aacagctaca
       61 cttgggagaa tataaaatca attgcagaag aagcagttga tgactattta aatgaactta
      121 acatgagttg ggaagatgaa gaaaacttaa tagttcgtat atctcagata gaaactcgtt
      181 tgttaagtat aaatggagtg ttagatatta caaatactat gataaatgat gttaaatcta
      241 atctaacaat agatagtaac agcatagtag cgagaggtga ggtagttgga taaagagatt
      301 aatctaataa attacttacc acaaattcta caagataaag aagaatatat aaaagtattt
      361 aatgcagaaa acaaagagat aaaaatatta catgataaac taaatgacct aacaagtgac
      421 cagtttttag aggatttaac accttttggt ataaaaagat gggaaaaaat aatgtctata
      481 actccaaagt cgaacgaaac tttagatgat agaagattta ggatttttag tagatatata
      541 agtaaattac cttactcaga gagattttta aggaactggc tagataatat agttggtgaa
      601 ggaaactatg agttaactat aaataatgct acttataata tacatttaga aagtgatgct
      661 agaaatcagg attggtttga ggaagttcat tcttttgtaa gtaatattaa gccatgcaac
      721 atgacactag attacactag agtgcttata agcaaagaca atcatatgaa ttttggtata
      781 acaactctaa tgggtcaaga aataactata tacccttgga gtccgccaga tatagaaact
      841 tatggagaaa ttgatgtatt aactggcaat ggagttggat accaagaggt aacaatattt
      901 taggaggtga tatattggct atagataaaa gttattacac tataattaca gatgtaggaa
      961 aagcaaagat agcaaatgca agtgtcacag gcaataaagt aggttttgta aaaattcaac
     1021 ttggtgatgg tggaggaagt gaatatacac caacagaaaa ccaaacagct ttaaaaaatg
     1081 tagtttggga aggtaatatc ggaaatacaa ctacagatga aacagcacca aattgcataa
     1141 tattagagag tttaatacca tcaagtgtag gcgggtttat gataagagaa ataggatatt
     1201 tagatgatga aaataattta attgccattt ctaaatacaa agagtgttat aaaccttcta
     1261 tagaacaagg tgcagtagta gacatgaagg ttaaaactgt gcttattgta tctaatgtaa
     1321 ataatataga acttaaaatt gaccctacga taatttttgc aacactcaaa gatatacaag
     1381 agttagacac taaaattgat acaactaaaa cagaattaac aaacagtata gaaactgcta
     1441 aaacagagtt aagcaataaa ataggtgata caacacaact taaaacaata gataaaacta
     1501 gtcttgttgg tgcattaaat gaggttaaaa ctagtgtaga tagcatagaa acaacagcag
     1561 agaaaacaag ttataataat gcaacaagta atcttactgc tacaaa
//
LOCUS       DAEMHZ010000264         1578 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000264 DAEMHZ010000000
VERSION     DAEMHZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.268"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            21..>1578
                     /locus_tag="KPK72_003817"
     CDS             21..>1578
                     /locus_tag="KPK72_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490957.1"
                     /translation="MQTEWNFNYDNYVQNVSLFPGKYKLECWGACGGAVNTSDWTDCA
                     KGGYSKGEIVLKKRTNLQICVGQSGYEQVPEGSSLTRSGFNGGGVAGKITTGRFAYSK
                     YGGGATDIRLYQPYATWDNTKSLLSRILVAGGGGGMKNNFASARSVGHGGGYVGANGI
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGVGGGGGWHGGAGSYFNECGGG
                     GSGYALTKDSYKPPGYIPTSEYYLENVVMTTGSNTTKADGYAKITLLQALPFLTVSSY
                     NSTTATFKADHTDPTLLTKIEIFIDDVLKETITEDLTTEKTINYTLEDNALHTLKIVV
                     TDSANATAEKVVSISKGIAPLPAGFTTDEVTNKWIEIKDTFKSGKTSIINTLALKNID
                     ASLNNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNTKYKYASGTIDVVKN
                     SSLMANLYGESFGRQPGTWLKIDNLGFIPNIFVAECQYVTSNNYFFKHIVIATCNINW
                     FWDKKDFSARI"
BASE COUNT          545 a          208 c          333 g          492 t
ORIGIN      
        1 taagagagga gagtgagaaa atgcaaacag aatggaattt taattatgat aattacgtgc
       61 aaaatgtttc tttgtttcct ggtaaatata aattggaatg ttggggtgct tgtggaggtg
      121 ctgtcaatac aagcgattgg acggattgtg caaaaggtgg ttattcaaaa ggtgaaattg
      181 tattaaaaaa aagaactaat ctacaaattt gtgtcggtca atctggttat gagcaagttc
      241 ctgaaggttc aagccttact agaagtggtt ttaacggtgg aggtgttgct ggcaaaatta
      301 cgactggtag atttgcttat tctaaatacg gtggtggagc aactgatata agactttatc
      361 aaccttacgc aacttgggat aacactaaaa gtttgctttc acgcatactt gttgcaggtg
      421 gtggcggagg gatgaaaaat aattttgcat ctgctcgttc cgttggtcat ggtggtggtt
      481 atgtaggtgc taacggaatt ggtcgtgaca gagatttttg tggtggtggt tctcaatacc
      541 aaggtggaac aagttacgat acagaagaat accatggttc actaggaaaa ggaggttatg
      601 gtaacatagg agtaggtggt ggaggtggtt ggcatggtgg tgctggttct tattttaatg
      661 aatgtggagg tggtggaagt ggttacgcac taactaaaga tagttataag ccacctggtt
      721 atataccaac atctgaatat tatcttgaaa atgtagtcat gacaacagga agcaatacta
      781 caaaagcaga tggttatgct aaaataacat tacttcaagc attacctttt ttaactgtat
      841 cctcttacaa ctctactaca gcaacattca aggcagacca cacagaccct acattattaa
      901 ctaaaataga aatatttata gatgatgtgt taaaagaaac tataacagaa gatttaacaa
      961 cagagaaaac aattaactat acgttagaag ataatgcact acacacactt aagatagttg
     1021 tcacagacag tgctaatgct acagcagaaa aagttgtaag tataagcaaa ggaattgcac
     1081 ctctccctgc tggttttaca acagatgaag ttacaaataa atggatagaa attaaagata
     1141 cattcaaaag tggcaaaaca agtattataa atactttagc attaaagaat atagacgcaa
     1201 gtttaaataa taccttagtt gagttatcag agaaaattaa aacttctttt gatagttcag
     1261 acgctagtgt acaagatttg atgaatcagt taacacaagc taataatact ataagtcagt
     1321 taaatacaaa atataaatat gccagtggta ctattgatgt tgttaaaaat agttctttaa
     1381 tggctaattt gtatggagag tcatttggta gacaacctgg tacttggctt aagattgata
     1441 atttaggttt tattcctaat atttttgttg ctgaatgcca atatgttact tctaataatt
     1501 atttttttaa acatattgtt attgctactt gtaatataaa ttggttttgg gataaaaaag
     1561 atttttcagc tagaattg
//
LOCUS       DAEMHZ010000265         1494 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000265 DAEMHZ010000000
VERSION     DAEMHZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.269"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            166..1275
                     /gene="ugpC"
                     /locus_tag="KPK72_003818"
     CDS             166..1275
                     /gene="ugpC"
                     /locus_tag="KPK72_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG2490958.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLILSVDNSRVHIFDKETTLAIR"
     gene            1305..>1494
                     /locus_tag="KPK72_003819"
     CDS             1305..>1494
                     /locus_tag="KPK72_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG2490959.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQS"
BASE COUNT          621 a          191 c          215 g          467 t
ORIGIN      
        1 tacataaaac ttttctaaat taaaatacat tttttaaatt aaaaaactat tagtctaaat
       61 ttttaatata catattaaac aaatacattt agtcatttta cacatttaat ataataaaaa
      121 aatttgatac tatataatta aaatttaaat atcaaggagg atattatgtc agaagtaatt
      181 ttaaaaaata tatcaaaact ttactcaaat ggatttaacg cagtaaaaaa cataaacata
      241 gacattaaag ataaagaatt tatcgtttta gtaggaccat ctggttgtgg taaatcgact
      301 acacttagaa tgattgctgg tcttgaagaa atctctgaag gagaactata cattggagat
      361 aaacttgtca atgatataga accaaaagat agagatatag ctatggtttt tcaaaactat
      421 gctttatatc cccacttatc tgtttacgaa aatatggcat ttgcacttaa acttagaaaa
      481 ttacccaaag atgaaataga caaaaaagtt aaagaagctg ctaaaatatt agacttacta
      541 cctcttttaa ataaaaaacc taaaacatta tctggaggac aaagacaaag ggttgcctta
      601 ggacgtgcaa tagttagaaa tcctaaggta ttcttaatgg atgaacctct atccaactta
      661 gatgcaaaac ttagaactgc tatgagaaca gagataacta agctacacca acagcttgga
      721 actacattta tctatgtaac acacgaccaa gttgaagcta tgactatggc tgatagaatc
      781 gtagtaatga aggatggagt agttcagcag attgcaactc cacaagatgt atacgattat
      841 ccagctaata tatttgttgc aggatttatt ggagcaccac agatgaattt tattgatgta
      901 atattaatag aagaaaatga tgaaatttat gcacaaaatg aacatattaa gcttaaattg
      961 aataaagaaa aacactatga tttaataaaa gacaattata ttaataatga agttgttata
     1021 ggtatcagac cagaagatat tcatgtagaa gatactttta ttaaatctaa tccagatact
     1081 tgttttaaat caagaattga aataaaagaa cttatgggag cagaaactta tgctcactta
     1141 aaactaggcg aaaatacgat aacaattaga tttgacagta aaaacagaat taatgttggt
     1201 gatgacttaa tactttcagt tgacaatagt agagtacaca tatttgacaa agaaactact
     1261 ttagctataa gataagaata taatcttatt tttaaggaga ctttatggaa gagttaaaag
     1321 attttttaga aagacagctt aataaaaaaa taaatatcta tccatatgaa aaccaaaagt
     1381 tagatgctag tagccattta gtcactttca ataatgctat ttatgttatt gattttctag
     1441 ataaaaataa tctcgataat ttaaaaggca acttttattt aatttaccaa tctc
//
LOCUS       DAEMHZ010000266         1451 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000266 DAEMHZ010000000
VERSION     DAEMHZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.270"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1231
                     /locus_tag="KPK72_003820"
     CDS             92..1231
                     /locus_tag="KPK72_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2490960.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMKNSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEGIEILSKEKNIYKVNDKYLKMLDID"
     gene            1257..1445
                     /locus_tag="KPK72_003821"
     CDS             1257..1445
                     /locus_tag="KPK72_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490961.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          637 a          121 c          215 g          478 t
ORIGIN      
        1 aaattgagtt tgataattat aaaacaaata atcaaaaata gtgtaaaaat taatactttt
       61 tgttaatatt aatagataaa gataatctaa ggtggtgatt aaactgacaa tagaagaata
      121 caaaaacaaa tactttactg gaaataaaat cgtactttct ataaatagtt ttgataataa
      181 agaactatac tatcatgaaa ctgaaaatat attaaattta ataaatgaaa tagataaatt
      241 aacatcaagt aagatattta aaacaataga agataaaaat gatttggcac tagagtctta
      301 ttattctagt gtaatagaag gtgctttttc aactagaaag attgcaaaat ctataattag
      361 aggaaagatg aaaccaagta ataaaagtga atatatgatt tacaataatc atagagcctt
      421 agaatatgga ttagacaact tagataaatt atatagtcac aaatttatat atgatttgca
      481 tcatatattg ggcgaaaact gcttggattc agaagaatat gagtatagaa ctgaaaaagt
      541 atatgtatgt gattcaaaag gtgagattat acatacaggg cttgaacctt tgaagatata
      601 tgactttatg agtaaattaa tagattttat gaaaaatagc aaagtaagta atttaattaa
      661 aagtgctata attcattttt actttgtata tgttcatcct tttagtgatg gtaatggaag
      721 aacatcaaga gccttatcat atctatattt gattgataaa ggatatgata ctttcaagga
      781 gttttcaata tcatatatga tatctaaaaa cagaactaag tattataagg ctatacttga
      841 tgtagaaaac aaaggaaata atttaactgt atttatagaa tttatgctca agtctataat
      901 acaatctatt aatgagatgc gtaatatgca tgatagaaaa tctttagaaa gtatattaaa
      961 agaggaattg tttgaaaatg acataactct atcagcaaca gaagaacata tattaaaata
     1021 tatttgcaat aaagataact attctatgac attagaaaat tatataaaaa agaataaaag
     1081 tagatactta aaggctggaa taaaggaaat tgaattagtt gaccagttga tggaagtgtt
     1141 taataatctt gaagggatag agattttgtc taaagaaaaa aatatatata aagttaatga
     1201 taagtactta aaaatgcttg atatagacta atttagataa tctggagagg gaatatatga
     1261 ataataagaa aaaggcattt ttgatttctg gggctataaa cgtaatttca ttaattattg
     1321 ttttttcatt gtttcattat aatattatag aaaatagtac acaaaatatg ataataactg
     1381 taatcttaat ttttattttt gaaatgataa aaataaaaat catagagaaa tactataaac
     1441 tataaacaag a
//
LOCUS       DAEMHZ010000267         1367 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000267 DAEMHZ010000000
VERSION     DAEMHZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.271"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..1242)
                     /locus_tag="KPK72_003822"
     CDS             complement(64..1242)
                     /locus_tag="KPK72_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2490962.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYRLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT          476 a          146 c          166 g          579 t
ORIGIN      
        1 tgatagatag aataagataa aaggcatagt aagattacaa gtaagctcat gtaaatattc
       61 attttagtta ttgttccttt tatgattatt ttgtttatta gtgtacattc taatatcagc
      121 aatattaata atttcttcta cagtcatttt gttttcgcta gaaacttcta caataccata
      181 gctaaaagat atcttattat ccataatttt acttattttc tctataaatt cttttgtggt
      241 gttttcatta gatttaggca taataactat aaactcatct cctcctattc tagcaaatat
      301 gtcatctatt cctaagtttt caataactat acttgagaag ttaataatat agttatcccc
      361 agctaaatgt ccatatgtat catttatcgt ctttaattta tccaaatcga tatatatcaa
      421 tgaaaataat aaattgttat caatgtaatg ctgtattttt tcaaaagcaa attttcgatt
      481 atatgctgat gttaattcat caatgttagc ttttttcttt aataaattta cttttagtaa
      541 ttgttcttgt tttatcattt ttagtctatt atgttctttt tctaaataaa tatttttttc
      601 aatggaatat atatgattga tgaatataat tagttgtaag attaataata tgttacttcc
      661 caatataaag taggaagtta gagaacttgg tggagcaaat gtaatttgta tactatcaaa
      721 taatatatat cctaaagtga accagataaa ttttaaaaat agactgaact ctatttgatt
      781 ttgagttatt atataaatat gctcttttat tttattcata gagtaccata agataaaatc
      841 taaaatagaa gctattaaaa tactaattaa cctataggtc atatttgcaa atattatatc
      901 tggacttgta tttttaacaa gtgatattgt acttacagta ataaggtgta aaatacaaaa
      961 acttattact gccatattgg gaataaaatg gttgttagta gaactttttc tgcttaagag
     1021 caatttatga cttactatgt aaaacaatat ataatatagt ggtaatggta attttgtgaa
     1081 tattattatc aagaaggtta atggttttat taaaaaaata tatttgtaat tcttatgtat
     1141 agttatattt ttgtttctaa aagatgctat tttgcatatg taaatgttac atagttcgat
     1201 tattaatata gtagttatga ttaaaaatat atggtatttc atagtacatc ctcttttaaa
     1261 gtgaaattgt atttacttaa aatagattag attttatata ttctatttta cagtttttat
     1321 ttaggtacac ttattatgga taccttcttt tttattgcat gtgtctg
//
LOCUS       DAEMHZ010000268         1355 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000268 DAEMHZ010000000
VERSION     DAEMHZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.272"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1355
                     /locus_tag="KPK72_003823"
     CDS             <1..>1355
                     /locus_tag="KPK72_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490963.1"
                     /translation="FKDGKNKELEIEYEKERFVTESYLLNKEINNKINLKGIKLNSAG
                     DKSLLSAINLISKGYKVSIFIPQKNSAKSGINKICIKLLNLIDRLNGKILYDKNCIDD
                     SNLLDRAELLLGRESIYMKLFRLGVIVIHSDMPQEVIEYMRYFIQCGKYKIIIGDKIL
                     TKNLSKYTDVLIINSIRINIDKSWSIIDDRLLLDLINDFSDQINKKHIIISNYKQKDI
                     YKTKIKNIKKGLILNSFISQSLHNIELFKLNYEEEINNILDSIEYNICEYILYDYSNE
                     FLDNLIYTNNQVDFIKEGQFVKLLNKKIDKLKSLSKDKIAVIYKSKANLSCLDMYIDL
                     KNKYINFDDIELINYIFYNYIVVNKEYVNIISNIEYIYKLNINEDGIYNIILHWISEY
                     DYNDIDTLTKINNLEIIFTVIEKVVRGLFVEMLCKLQNICKINDSTKLHNKLQYIKDI
                     L"
BASE COUNT          626 a           85 c          183 g          461 t
ORIGIN      
        1 ttttaaagat ggaaaaaata aagagcttga aatagaatat gaaaaagaga gatttgttac
       61 agaaagttat ttgttaaata aggagataaa taacaaaata aacttaaagg gtattaaatt
      121 gaacagtgca ggagataaaa gtttactatc tgcaataaat ttaatatcta aaggatacaa
      181 agtatcaata tttataccac agaaaaattc tgcaaaaagt ggtataaata aaatatgtat
      241 aaagctatta aatttaattg ataggcttaa cggtaaaata ttgtacgata aaaattgtat
      301 agatgattca aacttattag atagagcaga gttattgtta ggtagagaaa gtatatatat
      361 gaaattattt agattagggg taattgtaat tcattctgat atgcctcaag aagtaattga
      421 atatatgaga tattttatac aatgtggaaa atataaaatt atcataggag acaagatttt
      481 aactaaaaac ttaagtaaat acacagatgt gttaattata aatagtatta gaataaatat
      541 agataaatca tggtctatta tagatgatag attactatta gatttaatta acgatttttc
      601 agatcagatt aacaaaaagc atataataat tagtaattat aaacaaaaag atatttacaa
      661 aacaaaaatt aaaaatatta aaaaaggatt aatattaaat agttttattt ctcaatcatt
      721 acataatata gagttattta aattaaatta tgaagaagaa attaataata tattagatag
      781 tatagagtat aatatatgtg aatatatatt atacgattat tctaatgaat ttttagataa
      841 tttaatatat acaaataatc aagtggattt tatcaaagaa ggacagtttg taaaattatt
      901 gaataaaaaa atagacaaac taaaatcatt aagcaaggat aagatagcag ttatatacaa
      961 atcaaaggca aatttatcgt gcttagatat gtatattgat ctaaaaaata aatatattaa
     1021 ttttgatgat atagagttga ttaactatat attctataat tatatagttg taaataaaga
     1081 atatgttaat attataagta atatagagta tatttataaa ttaaatataa atgaagatgg
     1141 catatataat ataatattac attggataag tgaatatgat tataatgata ttgatacatt
     1201 aactaaaatt aataaccttg aaataatatt tactgttata gaaaaggtag taagaggttt
     1261 gtttgttgaa atgctatgca aattacagaa tatatgtaag atcaatgaca gtacaaaatt
     1321 acataataaa ttacaatata ttaaagatat attat
//
LOCUS       DAEMHZ010000269         1314 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000269 DAEMHZ010000000
VERSION     DAEMHZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.273"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(157..423)
                     /locus_tag="KPK72_003824"
     CDS             complement(157..423)
                     /locus_tag="KPK72_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2490964.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(465..1274)
                     /locus_tag="KPK72_003825"
     CDS             complement(465..1274)
                     /locus_tag="KPK72_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490965.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNKLFSLEVDSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          459 a          191 c          150 g          514 t
ORIGIN      
        1 tttccaattt tagtaatcag attttatcaa taaattacta ttctattaat ttaattaaat
       61 aacctttaaa tttaattata aaaactgttg cagaaatttc tacaacagtt taaaaaattt
      121 accttacaaa aacttatttt ataacaagag agtatattat aaggctagta cagcccattt
      181 tttgcactga gaaattcttt tgccatcatc accttgttct ttgtcatatg caaattgatt
      241 aagcatttca catggaaatg tatcacatag accacaatgt gtaaggtttt tatcttcaca
      301 acaagatttt acaggacatt gttcacccca aaatggtttc tcaatctctg tacaaccttt
      361 acaattgact ttttccttgt atccacattc ataacataaa attccacatc ttgattctat
      421 cattttaagt cactccttaa atataaattt tatatgattg tatatcaaaa tttatacttt
      481 tcattgtaaa aatcggacaa attttttata taggttgttg gattaacacc acaaatagat
      541 ttaaaatcat ttataaaatg agattggtcg taataaccaa tatcttgtgc taaagttgca
      601 aatggtattt tattattttg tatttcttgt aatacaaggt ttatacgaag taatctaagg
      661 tatgatttaa cactcatacc aagagaatta ttaaaaattc tatttaaatg tcgttcacta
      721 taacaactaa tttgtgaaat gtttttaaca gacataatag agttttgttt tctagcattt
      781 tcaagaattg gtatagtcat atctactata tttgatttca ataagtatga taagaataac
      841 atatctaatt gttctactaa ttcctttata gtacttgttc tttcaaaaat ggaatcaacc
      901 tctaagctaa ataatttgtt aaaatcttca agtgaaattt ttaaatctgt agattctctt
      961 tgagaaagac ctgtcaaata atatactcca cctgggcgaa actcaacgaa tactctaaaa
     1021 agtacatttt ctatatcatt ttttacaata gtagtttttg ttgtggctcc ccagaatgcg
     1081 ctttctatac cttttttgtc aaatttaaat atcatacatc cactagcatc aggtattaat
     1141 gtaagatttt ctttaatact tgtatctgga atagatattg tatagtgtgc tatatactgt
     1201 cttaacaatt tatgtggcaa aatatatgta tactgtttgc aggtatttaa aatgttattg
     1261 attattttat tcataagtat atcctcctga tatataaaaa ataagtatac taat
//
LOCUS       DAEMHZ010000270         1288 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000270 DAEMHZ010000000
VERSION     DAEMHZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.274"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..129
                     /locus_tag="KPK72_003826"
     CDS             <1..129
                     /locus_tag="KPK72_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBG2490966.1"
                     /translation="DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            139..1020
                     /locus_tag="KPK72_003827"
     CDS             139..1020
                     /locus_tag="KPK72_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2490967.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     EHPQELKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQDKKQSNSKAVNTHNVSKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            1080..1253
                     /locus_tag="KPK72_003828"
     CDS             1080..1253
                     /locus_tag="KPK72_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG2490968.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIGREKQNSKTTY
                     TYFVEYTEEQEEK"
BASE COUNT          555 a          134 c          260 g          339 t
ORIGIN      
        1 gataaaaata gagttgatag tgaagtatat aagaaatttg gaaagttagc agtagatttg
       61 actaagcaag agtatgagaa agttttaaat ggatataaga gcattttaga gaagcaaaaa
      121 caagagtagg tgatagtatt ggggattata agagtaagca aagacaaaga taatccatat
      181 gtagttttaa ataaaacttg tttggaagat gtaaaattaa gctggcaagc aaaaggttta
      241 cattcatatc tgattagtaa gcccgaccac tggaaaatct atgttaatga cctttgcaag
      301 agaagtaaaa atggaagaga tgctacagca aatattttaa aagaacttat agaaaatgga
      361 tatataacaa gaaaaccttg tagagattct aatactaata aaatgcttgg agggtatgat
      421 tatgaagtat atgagatacc gcttgaacat ccacaagaat taaaatcccg aaaaactgat
      481 ttcccggaaa ccggatttcc cggaaaccgg gtttctcgga aaccggaaaa cacggaagta
      541 gtaagtaatg actttaaagt aaataatgat attactacta ttgttattaa tgaacaatcc
      601 aataaagaca aaaccaccta cataaaaaaa tactttgaaa aatatatagg tgtgattact
      661 cctaataact ttatagagtt aatgagttac ttagatgatg gaatggaagc tgatgtaata
      721 ataagagcta ttgatgaagc aatagcaaat ggagttaaga attataagta tgtaaagaca
      781 atattaaata attggataga agcaggtgta aaaactaatt tagaacttac agagtatcaa
      841 aatgagtttg aaaggaagaa aaagaataaa caggataaga agcagtctaa tagtaaagct
      901 gtgaatactc ataatgtgag taaaaataag tttgctaact tcaatcagac tttcactcaa
      961 tatgaagaaa aagagctaga tgagattatt aaaaagagtc agaaggaaaa atttaaataa
     1021 aattaaactt ctaggaagta aatatcaata tattgcttcc tagaaagggg aggtataaaa
     1081 tggcgagaat atatgcacaa agaagtggtt ctttaaatga gcaggataga ttggaattgt
     1141 taagattact tgggaaagct ggatatacag taaagattgg tagagagaag caaaatagca
     1201 agacaactta tacttacttt gttgagtaca cagaagagca ggaagaaaaa tagaaggggg
     1261 ctagttaaat gaatacaata actttagt
//
LOCUS       DAEMHZ010000271         1280 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000271 DAEMHZ010000000
VERSION     DAEMHZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.275"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..410
                     /locus_tag="KPK72_003829"
     CDS             <1..410
                     /locus_tag="KPK72_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2490969.1"
                     /translation="LGVAGIIFLCMIPMFLYDFSSDKVIALIARILYFSDYFIVILFL
                     IFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECINGYNLNTNNIRIFGTDSGGISP
                     LMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            421..636
                     /locus_tag="KPK72_003830"
     CDS             421..636
                     /locus_tag="KPK72_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2490970.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVENEND"
     gene            1077..1250
                     /locus_tag="KPK72_003831"
     CDS             1077..1250
                     /locus_tag="KPK72_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBG2490971.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
BASE COUNT          475 a          143 c          161 g          501 t
ORIGIN      
        1 ttttaggagt tgctggtata atattcttat gtatgatacc tatgttttta tatgatttta
       61 gttctgacaa agtaatcgct ttaattgcaa gaatactata tttttctgac tactttatag
      121 tcatcttatt cttgatattt ataattaata gtactaagta ttccccattc gtacttgaaa
      181 atgtaaaaag gtttaaggta atgggatttt gtttattgat aaacactata attgaatgta
      241 tcaatggtta taatttaaat actaataata taagaatttt tggaactgat agtggcggta
      301 tctcaccact tatgattatc tcttttatat ttgcactaat gtgctttgta attgcagaaa
      361 catttgataa agctataaaa ataaaagaag ataatgattt aacgatatag aggtgatgtt
      421 atggcaatag tagttaacct agatgtaatg atggcaaaaa ggaaaatatc acttaaagat
      481 ttggcagaaa aaatagatat tacaaatgca aatttatcaa ttttaaaaac tggaaaagca
      541 aaagctatta gatttaccac tctaaacgaa atttgcaaag ctttagactg tcagccaggt
      601 gatatacttg aatatgtaga aaatgaaaat gattaaaaaa gagtgtttag aaacactctt
      661 ttttattata ctatatgatt tttattcttt tactatatta ttgacattaa tattaacttt
      721 gtttaaagtc ataacaaaat atgttctaag aaagaaactg ttcctttatc tatttgttat
      781 ccattaccta taagaactgt atcacactaa ataatacttt taaattgata attttaccac
      841 agattcctat atacattctt ttctacctaa tatttattta aactgttagc aaaacatgga
      901 actttttcat ttacttatac ctccatttat attattgcat agtttatagc tacgtgttat
      961 aaattacatt attgatgatt ttatctaaca tttgtaatct tttagatact ctttgaagtt
     1021 acaacactgt tatactctaa ttaagtaaac taaatctgat acaagttggt gattatatga
     1081 aaagaaaact ttcaacctat tttaaaggat atattttagg tttatgtata gcatttttat
     1141 tatttacttt tatattaaaa aaattaatta tagaaaatat cattggaata ataattggaa
     1201 ctggaattgc atttttactg atttacctat ttaataaaaa aagaaaataa tattctataa
     1261 aaaggtcttt tagtgaattt
//
LOCUS       DAEMHZ010000272         1255 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000272 DAEMHZ010000000
VERSION     DAEMHZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.276"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..758
                     /locus_tag="KPK72_003832"
     CDS             99..758
                     /locus_tag="KPK72_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2490972.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
     gene            1052..>1255
                     /locus_tag="KPK72_003833"
     CDS             1052..>1255
                     /locus_tag="KPK72_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG2490973.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYI"
BASE COUNT          491 a           93 c          160 g          511 t
ORIGIN      
        1 tattaaattt aagtgtgttt tttatgctat aattagtatg tattttttat gatatatttt
       61 ataaaaaata taatttaatc atttttgagg atattattat gttagatgca tttataacaa
      121 ttgtttcttc tattatagaa atatggtcat gtaaaaaagt ttttgattat actagtcagt
      181 caaagaccga cttaaaaaaa ctaaatatgt attttctctt ttctgttttt ctcattattt
      241 cttctttata tataaatata ggagctaata gtagaatatt gatatgtata tttgtaatgt
      301 tcgttttcta taagtctaac tataaggtta atatatataa atgtctggta atctcatttt
      361 tttattggtt aatattaata ggtataaatg gattaagtat gttattagta gcaacagtaa
      421 gcaatttgga taatatgaat gttttactta gtggcagttt atatagatat gaagcaatat
      481 tcttgggaaa gataatgtta ggtatattac tttatgttta taaggcttct tttaaaaatt
      541 ttaaaataag taaaaaagag atatacatat gtattcctat gatagcaaat ttattgtcat
      601 tttttgtact ttatgattat attattaatc ttgtaaaaat ctctcctgaa agaagaactg
      661 cttttttagt aatcgtttta ctattaatat tatctaacat ttcaatcttt gtattaatac
      721 atataatact taagattaaa attaggaaaa taagataaat tttaatacga ttcatttaaa
      781 ttaaaagtta ataattactt ttttatgtat aattttacat gaatttctat aaaagttatt
      841 ttattaaaga tagatatact tatatatacg gatatgatta aaaaaagtct ttttaacttt
      901 aaaaattaat gttaaaaaat aaacttatac actaatatta ataataatta tttaaatatc
      961 ttaagtaaat cagtcttaca tatatttccg atagattata ttgataaaaa acactaaaat
     1021 taaattaata aaagtacttg gagggatgga aatgaaagag gaaaatttta ttaatgttga
     1081 atacgaggat aaacgttttt caattaatag taaagaagtt ttgtacatag aaaagcaaga
     1141 gtatgaagta aagatttata ctgaaagtgg taattatgaa gttggagaaa atgctccttg
     1201 tataagaaaa ctattgaaag aagaaaattt tttcaaatgc agtaatagtt atatt
//
LOCUS       DAEMHZ010000273         1160 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000273 DAEMHZ010000000
VERSION     DAEMHZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.277"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="KPK72_003834"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="KPK72_003834"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="KPK72_003835"
     tRNA            179..253
                     /locus_tag="KPK72_003835"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="KPK72_003836"
     tRNA            259..344
                     /locus_tag="KPK72_003836"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="KPK72_003837"
     tRNA            360..435
                     /locus_tag="KPK72_003837"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="KPK72_003838"
     tRNA            443..517
                     /locus_tag="KPK72_003838"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="KPK72_003839"
     tRNA            532..605
                     /locus_tag="KPK72_003839"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="KPK72_003840"
     tRNA            611..686
                     /locus_tag="KPK72_003840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="KPK72_003841"
     tRNA            692..768
                     /locus_tag="KPK72_003841"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="KPK72_003842"
     tRNA            779..853
                     /locus_tag="KPK72_003842"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="KPK72_003843"
     tRNA            863..936
                     /locus_tag="KPK72_003843"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
BASE COUNT          323 a          256 c          281 g          300 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataacta
//
LOCUS       DAEMHZ010000274         1058 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000274 DAEMHZ010000000
VERSION     DAEMHZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.279"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(447..914)
                     /locus_tag="KPK72_003844"
     CDS             complement(447..914)
                     /locus_tag="KPK72_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2490974.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSSMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          417 a          169 c           63 g          409 t
ORIGIN      
        1 aaatttttaa gctaagattt tttgtcttta agaacaataa taatattatt atttatgttt
       61 cttatttaaa cattatttta tttgaaatat ttattaaata aataaactaa aactacaatt
      121 ttcactatat ttttaatttg ttcaattaaa ttttaacata attacataaa atagtatata
      181 tgattctaca gcttgatagt aagtataatt atcttttata agctaatact tatatttttc
      241 taaatttaaa tcatccaata aatattaaat tctattataa tatgtaagga aaatcatgca
      301 ctttttaaaa atccaaataa aattataaca aatattatat tctgtatctt acaaaaacaa
      361 aaactgtaag taaatttgtt tccatacaag tttacttaca gcccttactt ctatacaaaa
      421 tatagtttta ctttaatata atccatctat tctaaaactg attcaatata ttcgacagtt
      481 acatccattc ttttagctac ctcatctatt gaaagaccac tatctaaaaa acgtctacac
      541 acagatgaca tagtttctcc aacctcaata atatgcccat ctaaatcata aaatctaatt
      601 gctctttgac cccacctatg ctctatagct agatgaacat aatctatatc tttcattgaa
      661 ctcaatcttt caacaaaagt atcaaaatta tcttcctcaa aatataactc agcatcattt
      721 ccattgaact taacttcact gtcactctta ttaataaatt ctaaccaaag ctttttttct
      781 tgtaatgata tattaccttc taatacaaca ttttctccaa aatcacagat tacctttaac
      841 cccaaaacat cataataaaa cttttttgat ttctcaatat cttcaactac tattaaaaca
      901 tttaaaaact tcatattacc tccttaatat acacatactt tatttataat atataattag
      961 aattaaacta taaaggtttc aataaggtct agtataaaga aaaaaattat aagtcttatt
     1021 gaaatagtta agttaatttt atcatataat ataaattt
//
LOCUS       DAEMHZ010000275         1046 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000275 DAEMHZ010000000
VERSION     DAEMHZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.280"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            26..445
                     /locus_tag="KPK72_003845"
     CDS             26..445
                     /locus_tag="KPK72_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2490975.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSWNYGVELNDLIGEPISFVIPEIERRIKEALIQDDR
                     IETVDNFEFQNVKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            458..>1046
                     /locus_tag="KPK72_003846"
     CDS             458..>1046
                     /locus_tag="KPK72_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2490976.1"
                     /translation="MTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTETYIAM
                     DELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPLDSRFNLGEYNYT
                     ATERISEGIYKMKCETTGPIFELGQLIPIEYIDGLETAQLTEILINGEDAESEDSLRQ
                     RYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPV"
BASE COUNT          399 a          121 c          201 g          325 t
ORIGIN      
        1 ttgattttag ataggatgtg ataaagtgtt accaacagat aacattgact atgatattga
       61 agatgtatca ataattaact ttgatgtaag gcaagaacca agtaagacat ttaaacttca
      121 tatagaaaaa aacagagtag atggtatttg tgatgatgta gaagcattaa aacagaccat
      181 ctttttaatt ttaaatactg aaaggtatga gcaccttatt tattcttgga actatggagt
      241 tgagttaaat gacttaattg gagaacctat ttcctttgta atacctgaaa ttgaaaggcg
      301 aatcaaagaa gcactaattc aagatgatag gattgaaact gtagataatt ttgagtttca
      361 aaatgtaaaa ggaaaggtcc attgtaaatt tatggtccat accaaatatg gaaatatcaa
      421 agcagagaag gtggtgagtg tataattgtt tgagttaatg acttttgaaa atataattaa
      481 aagaatgtta gatagtgtac cagatacttt tgataaaaga gaaggttcta taatatataa
      541 cgccttggca ccagttgcta tagaacttac agaaacatac attgccatgg atgaattatt
      601 agaccaaacc tttgtagata ctgctagtta ttactattta gagaaaagat gcaaagagcg
      661 aggaatcaca cctcttgaag ccactaatac aattgcaaaa ggagttttta acatagatat
      721 tcctcttgat tcacgcttta atttaggaga gtataattat acagcaactg aaaggattag
      781 tgaaggaata tataagatga aatgtgaaac tactggacct atttttgaac ttggtcagtt
      841 gattccgatt gaatatatag atgggcttga aactgctcag ttaactgaaa tattaattaa
      901 tggagaagat gcagaatcag aggacagttt aagacaaaga tactatgata gtttaaattc
      961 tcaatctttt gggggtaata ttcaaaacta taaagatgaa gttaacaaaa tacaagatgt
     1021 tggaggagtt aaagtttatc ctgttt
//
LOCUS       DAEMHZ010000276         1012 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000276 DAEMHZ010000000
VERSION     DAEMHZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.281"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..242)
                     /locus_tag="KPK72_003847"
     CDS             complement(<1..242)
                     /locus_tag="KPK72_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490977.1"
                     /translation="MLNNIIDGISVKLDKSFGNEYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPNGRTLKKNSFDVHYFPKSNDKSFEINKIAE"
     gene            complement(235..660)
                     /locus_tag="KPK72_003848"
     CDS             complement(235..660)
                     /locus_tag="KPK72_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2490978.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(660..1007)
                     /locus_tag="KPK72_003849"
     CDS             complement(660..1007)
                     /locus_tag="KPK72_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490979.1"
                     /translation="MMNKTRKAIEMLYRDKCTIVEHQPIKDPVTKRTKNKEVIVLENQ
                     PCKLSYKNIMSTTDGKVAKLEQTIKLFISSDIEIKAGSKIIINNKEYVRSGESAIYPN
                     HQEIILELFKDKA"
BASE COUNT          284 a          191 c          114 g          423 t
ORIGIN      
        1 atctcagcta tcttatttat ttcaaatgat ttatcattac ttttgggaaa ataatgtaca
       61 tcaaaagagt tcttttttag tgttctacca tttgggtatg gtgtcttact tggattcaaa
      121 ggaacaataa aaaaacaagg ttcatttatc ccttgttcta cgtcttcgct gtaaattgtg
      181 tattcatttc caaacgactt atctaacttt actgatattc catctataat attattaagc
      241 atcaaacact cctttcaaca aaattaatag ctttttctct attatcttat caacttggct
      301 ttgtagttcc atctctgaga tagttaagaa atgttgccct tttacccaac cttttccatc
      361 tttagttcta tgcccgaaat ttacataact tgcatattca gttgggttaa caacttctat
      421 aatataatta tttccttgct tatatacagg aagtgaccta gcataagcta ctccattcca
      481 tccttgtcgt aagaatcctg tatcaactgg tgttcttcta attactttcc caagtaatcg
      541 tgctgctaat tctcttgctg catccttgca aaacttatct aaatcaatct ttgtaaactt
      601 ctccatcttt ttacaaactc ttttaaactc tctaaaatca acactgcccc atctagccat
      661 tatgctttat ccttaaataa ctcaagtatt atttcttgat gatttggata tatagcagat
      721 tctccacttc ttacatattc tttattattt ataataattt ttgaacctgc tttaatttct
      781 atatctgaag atataaagag tttaatagtt tgctctagtt tagctacttt cccatctgta
      841 gttgacataa tatttttata tgaaagttta catggttgat tttctaatac aatcacttct
      901 ttatttttag ttctctttgt cacagggtct ttaattggtt gatgttctac tatagtacat
      961 ttatctctat atagcatttc tattgctttt ctagttttat tcattatttc cg
//
LOCUS       DAEMHZ010000277         1008 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000277 DAEMHZ010000000
VERSION     DAEMHZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.282"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..205
                     /locus_tag="KPK72_003850"
     CDS             <1..205
                     /locus_tag="KPK72_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBG2490980.1"
                     /translation="LNYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKI
                     EKDYKNPQKIITIREVGYYFNKI"
     gene            654..938
                     /locus_tag="KPK72_003851"
     CDS             654..938
                     /locus_tag="KPK72_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490981.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          448 a           61 c          158 g          341 t
ORIGIN      
        1 attaaattac tttatagaga atgctgaaaa gactctaact attaaggaga tatatgaaca
       61 tgtatggatg aatgagtatt tagatgataa ttatagtgta gtggttgctg tgagtggatt
      121 aagaaagaaa atagagaaag attataaaaa tcctcaaaaa attataacga taagagaagt
      181 agggtattac tttaacaaga tataaagctt tataaacaga atgaatgata gggcatagat
      241 aaatatgtta gtatattaca agagtttttg ttctaacgat atatttgtaa aattagattt
      301 aatttactta attgaattat agcagtagaa taaatataaa aaatgtctga aatattatag
      361 acatcagtaa aatatataaa actataatat aagtcgtaat aataaaacaa atgaatatta
      421 attaataaaa agatagtagc tatttttata tagctattat ttttttattg ttgtggatga
      481 aaatatactt atattttact aatatacatt taaaaattat aatttaaagc attaaaaaat
      541 aaatttgtat tttgttttat ataaagtaat ttgaagaata tggaaaaact tgattagtac
      601 attattaatt agggtataaa atggtgtaaa tagaatgata aggggtggat gaaatgcttg
      661 tatataataa aagtttttat cctaatgaca tatttccaag attagatttt tcaaaaataa
      721 aaaaacagtt aaaattgata gataatgacc tgtcagattt tggaagcata tgtataatag
      781 aaaaagaaca ttatacaata agtgtaaaca gtataggtga aataaatgtg tattatgatt
      841 tagagtacga aaataaggtg tatagaatag tttatgagat tgaaaagtta tttaaatctc
      901 aagttggaag gtttagcata tctacataca gaaattgata attaaaaaag tagaaattaa
      961 aaaacttaat actaaagata tagataaata tatgaggtaa cagctaga
//
LOCUS       DAEMHZ010000278          978 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000278 DAEMHZ010000000
VERSION     DAEMHZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.283"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..823
                     /locus_tag="KPK72_003852"
     CDS             <1..823
                     /locus_tag="KPK72_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG2490982.1"
                     /translation="TIFAYSNIKYNNNKLIYSNMIDKKTQNSVKEILKENKINEKDID
                     TFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYMDVN
                     CRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSAIPA
                     KDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLRYPETKNVFIGHTGICIKTKN
                     GILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEGEIELPDPIIMENDKLMK"
BASE COUNT          445 a          101 c          116 g          316 t
ORIGIN      
        1 tacgatattt gcatattcaa atattaaata taataataat aaattgatat attctaatat
       61 gatagataaa aaaactcaaa atagtgtaaa ggaaatccta aaagaaaata aaattaatga
      121 aaaagacata gatacattta ttaaagcagt caacaattat aataaattgc aagttaaaat
      181 acttcaaaat aatataaata tatctaaatc tggatattct tcaatcaatg caaaacaagt
      241 tccatataac ctagaaaaat tgcaagataa ttgggtgaaa aaattccctg attatatgga
      301 tgtaaattgt agaataactg cttttagact ttttaaagat tttataaatt caaataagaa
      361 atttactgga gactctattg atttgaatgt cgacttggac actataatga ataataaaga
      421 tgctaaattt agcaccaaag atgtagaaaa atttatcaac ttcttttctg caataccagc
      481 taaagataca gatgatacaa tcaaaatttc agaacaaata aaaaatgaat ggaaaaaaag
      541 aaaaatatct tttaaaaata ataaaaatat atctattata aatggatttc ttcgttatcc
      601 agaaactaaa aacgtattta taggtcatac aggcatatgt attaaaacta aaaatggcat
      661 tctattttta gaaaaatatg gtgttacatc accttatcaa gttactaaat ttaaaaataa
      721 agaagatgta aaaaattata tgtttaatag attgaagatg tcagaaggtg aaattgaatt
      781 acctgaccct ataataatgg aaaatgataa acttatgaaa taataacttt aaacgcaaaa
      841 tagtatttat aattactaag tcaatataat agagaaggtc tatagatttg tgtaaataca
      901 agcttatcta caatcttata taagaatgtt tatataaata agatagatta attctaagtc
      961 ctattattct attgaatt
//
LOCUS       DAEMHZ010000279          971 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000279 DAEMHZ010000000
VERSION     DAEMHZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.284"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            524..820
                     /locus_tag="KPK72_003853"
                     /pseudo
     CDS             524..820
                     /locus_tag="KPK72_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          427 a           82 c          125 g          337 t
ORIGIN      
        1 gatttataga tgaagaacta aacttataga ttaaaaaatg aatgatttat agatgaagaa
       61 ctaaacttat agattaaaaa ataaatgact tatagatgaa aaatcaagct catagattaa
      121 aaaataaatg atttatagat gaagaactaa acttatagat taaaaaataa atgacttata
      181 gatgaaaaat caagctcata gattaaagaa taaataactt attagatgaa aaaccaagct
      241 tatagataag aagtccatgc ttataagcca gaaaatgaat agtttaatag attaaaaaat
      301 acatatttgt gaattataga atgaatgact ccataaacta gaaaatttat aataatatct
      361 atatgaaagt tagaattatt ttatcacaaa aaaattaagt taaataaata tgaaattact
      421 tccaaatata cttccaaaaa catgtaatcg catatataat ataattataa aaataataaa
      481 tatttttata gtagtagtta attattaata aggaggggtc aaaatgccta gcatgattat
      541 ggaagttgta ggagtactat ttatagctat tggattaatc ttgacattca ttgaaataaa
      601 aggaaatttc ccttcttttt atggttttgg tcaaaattaa aatgctgtag gtattatagc
      661 agcaggaatt gctttaattt tctttagaaa tggcatagat agcatagatt ctatagctac
      721 ttatggtgta tttttagcaa tagaacttgt tgtaatagtt gcatatttct taataagaaa
      781 aaaatttact aaagaagata aacatttaag aactaattaa ctaaattatc tactataaaa
      841 ttggaaaatt aaaaacaaat aaaagtttgt ttttaattat ttaaaatcca aatttgatta
      901 tttttaaaga aactatgttc tgtataaaac tagaatgtgg tttctttttt atgtataaaa
      961 tattttttag c
//
LOCUS       DAEMHZ010000280          912 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000280 DAEMHZ010000000
VERSION     DAEMHZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.285"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            30..218
                     /locus_tag="KPK72_003854"
     CDS             30..218
                     /locus_tag="KPK72_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490983.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMVEFIQESRKIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(198..515)
                     /locus_tag="KPK72_003855"
     CDS             complement(198..515)
                     /locus_tag="KPK72_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490984.1"
                     /translation="MSKNVDIKNLKRFQKKIEKIEQNSKSEFDKIRTIILNKKFMNQH
                     TNFDSFDELLTFGGYIVNSEEDFLAIPDNEFDSFIVENTDFPNWQTMLDTAYSKYLKS
                     CLR"
     gene            621..>912
                     /locus_tag="KPK72_003856"
     CDS             621..>912
                     /locus_tag="KPK72_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HBG2490985.1"
                     /translation="MKGEGAFMNIREIMNILKLERSEVRLYFTMKRGINYISYSPTIE
                     DGLQDYLKNLVCEYLLRFEDIEQVMFSPIGYKDETIETCDIEYIGCYEKVIES"
BASE COUNT          338 a           92 c          161 g          321 t
ORIGIN      
        1 atacaataaa ttaaaaggag aatgatatta tggagattaa gaaaaacaca caagatgtag
       61 tatcagaaag aaaaaattcc ctagattctg aattcaagat acctgcaagt ggtgtatgtt
      121 atatggttga gttcatacaa gaatctagga aaattataaa agaattagat aaacattttg
      181 aaagttgttt agatgtttta tctaaggcaa gactttagat attttgaata tgctgtgtca
      241 agcatagttt gccaatttgg aaaatcagta ttttcaacta taaaagaatc aaattcatta
      301 tcaggaatag ctaaaaagtc ttcttctgaa ttgactatgt aacctccaaa tgtaagtagt
      361 tcatcaaaag aatcaaaatt agtgtgttgg ttcataaatt tcttgttgag aattattgtg
      421 cgaattttat caaattcaga ttttgaattt tgttctattt tctcaatttt cttttgaaat
      481 cgttttaaat ttttgatgtc aacattttta ctcataataa cacctccttt cagtaaaatt
      541 ttagcataat aacacagctt attcttcgat tgtcgaacga ttgctggagg atattatgtt
      601 ttagtgtaga aatttatatt ttgaaaggag agggagcatt tatgaatata agggaaataa
      661 tgaatatttt aaaactggaa aggtctgaag ttagattata tttcactatg aagaggggga
      721 taaattacat aagttattct ccaacaattg aagatggatt acaagattac ttgaaaaact
      781 tagtgtgcga gtatttattg agatttgagg atattgagca ggttatgttt agtccaattg
      841 gatataaaga tgaaactatt gaaacttgtg acatagaata tataggatgt tatgaaaaag
      901 ttatagaaag tt
//
LOCUS       DAEMHZ010000281          893 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000281 DAEMHZ010000000
VERSION     DAEMHZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.286"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..367
                     /locus_tag="KPK72_003857"
     CDS             191..367
                     /locus_tag="KPK72_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490986.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          353 a          111 c           92 g          337 t
ORIGIN      
        1 atgttgaata atgaagcatc ttctagtatt atatacttgt agaataaaac taaattggta
       61 aaattgggaa tatttaacct taaggagtta agttatagaa actagtacta aaaaatttta
      121 taaattatgt taattgacta atagaatgcg actttttatt tttactaaat tttttagggg
      181 gatggataat atgtttattg gagaaataga caaatgtaca catatactaa ctgcttacat
      241 cagtagttca tatgattatt gtaatttttt agatacacag ttagatgatt ttatatcaga
      301 atatggagaa acggtagttg aaacctgctt acatcaagtc ttattattag taagtagata
      361 caattaaaac taaatttttc tagaaaaacc tttttagatt tatctactct aataaatata
      421 taattttttg tactttataa gtaactataa cttctgctaa agtaaagatg tattgggagt
      481 acatctttac tttcccatta tagaagttaa aagcatacac cataaaatca atcatccaat
      541 acaaattata tatttcctat gtctaactta atttacaaga atcattctat atgcatattc
      601 tgcaatccac taaacatggc tctaaacctt tactatttta tacattctct ataaatcata
      661 caaatcatat ataaaatccc cctaatatgt ttaaaccacc tataaaataa caccaatatt
      721 tagaatattc aatatcaact gcataactgg tcaataaaaa cgtataacta gacaaatttc
      781 aatatttata taaataatac aagaaaaaca agcattttat ttatataagt tttttatttt
      841 ttgtttatat taaaaaacta tattgattaa tttattttat ttagtaatta taa
//
LOCUS       DAEMHZ010000282          822 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000282 DAEMHZ010000000
VERSION     DAEMHZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.287"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..549
                     /gene="srtB"
                     /locus_tag="KPK72_003858"
     CDS             <1..549
                     /gene="srtB"
                     /locus_tag="KPK72_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG2490987.1"
                     /translation="KEYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDF
                     YKKDSISGTLFMDYRNKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKIT
                     LNGKEFLYDVFSAYIVESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKI
                     VTLSTCTYEFDDARMVIHGRLI"
     gene            complement(709..783)
                     /locus_tag="KPK72_003859"
     tRNA            complement(709..783)
                     /locus_tag="KPK72_003859"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(749..751),aa:Thr,seq:tgt)
BASE COUNT          348 a          105 c           95 g          274 t
ORIGIN      
        1 aaagaatata aaaaagagga tttatcaaaa attaacagtg attttaaatt ttggctttct
       61 gttgagaata caaatataaa ttatccagtt gtacaatcta aagataattc ttactatctt
      121 gacaaagatt tttataaaaa agattctatt tctggtacac tgtttatgga ttatagaaat
      181 aaatctattg atgacaaaaa tatcataata tatggacata atatgaaaaa taaaactatg
      241 ttcaacaacc taaataagtt taaagatgct gacttcttta aaaaaaataa taaaataaaa
      301 ataaccttga atggaaaaga atttttatat gatgtttttt ctgcttatat tgtagaatct
      361 gattatgatt atctaaaaac aaattttaat aatgaatctg attatcaaaa ttacataaat
      421 gatattactt ctaaatcatt gtataaatct cctattaagg taaatagcaa tgataaaata
      481 gttacacttt ctacatgtac ctatgaattt gatgatgcca gaatggtgat tcatggtaga
      541 ttgatttaaa actcacagac acagtttaaa acaataattt taaactgtgt catcttttat
      601 acctataatt tataaattag accactaaaa gtatttaata caaaataaca aatacatcaa
      661 acaataaaaa acaataaaaa aagactatgc taaaaacata gtctttactg gagctggtga
      721 taggaatcga acctacaacc tgctgattac aagtcagctg ctctaccgtt gagccacacc
      781 agcacaccta ttttattgta aattagtaaa actaattttt aa
//
LOCUS       DAEMHZ010000283          794 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000283 DAEMHZ010000000
VERSION     DAEMHZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.288"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            460..>794
                     /locus_tag="KPK72_003860"
     CDS             460..>794
                     /locus_tag="KPK72_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2490988.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYPLLIVL
                     KLLNLINNN"
BASE COUNT          327 a           72 c           65 g          330 t
ORIGIN      
        1 tataatcata tagtaaatta aaatcatttg atttatttaa ttattattta ttaaccagac
       61 gaatgtaata attattaaat ataaaatatt aaaatcaaag agaataataa atgtaatatt
      121 aaaactcaat aataaattta tattcataag tttaatttct tcattaattt tatcaactat
      181 gcctctactg cctattaatt taatgcaata aataataaat cttttacaaa aagataataa
      241 aaatatagaa aaatattttt tattgtcctt ttggttatgg taaaccacat tctgcctgca
      301 atataactaa atatatgggt agatagtaaa attaatttta ttatttatat ataattttat
      361 aagttacttt tcaataatat tgactgtcat gaatttataa tattcaataa aatattttaa
      421 tttagttaag aatctatatt gtaaaggagt attaaattaa tgaacttaaa attaatatgg
      481 ataaatattc taaacccagt atttattaaa aatgaaataa aaagaaaaaa aatacaaatt
      541 attatctttt tctttataaa ctttggttta acttattcat taggtttact gttatattat
      601 aataaattta ttgatccaga aaactttgca tcattcatga tgatattacc tctatcttca
      661 gttgctattg caaagtttta tactgaagga ttaaataatg atatgtatga attttattca
      721 ctaatcatat tattttttct tatttatcca ttattaattg ttcttaagtt attaaatcta
      781 ataaataata atga
//
LOCUS       DAEMHZ010000284          789 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000284 DAEMHZ010000000
VERSION     DAEMHZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.289"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..558
                     /locus_tag="KPK72_003861"
                     /pseudo
     CDS             136..558
                     /locus_tag="KPK72_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            572..>789
                     /locus_tag="KPK72_003862"
     CDS             572..>789
                     /locus_tag="KPK72_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2490989.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESTSPLKVKIEQKL
                     SIGEFFLIQTDTFKKYTDKKIGDKLVLI"
BASE COUNT          331 a           99 c          162 g          197 t
ORIGIN      
        1 agccctctta ataaagattg ggtaccagga gtgggcaaat tttataaaca aataacagga
       61 agtacctata gttctaataa tgcaggtaca ggagttggaa gcaacgagga agcagaaaag
      121 aatttaaaag atgtaactta tcaaattcaa ggtaacaacc aaagcagtag tacaaataac
      181 aattctaaag cagataaact aattagtgta gcaaaaagta aactgggttg taattatgtg
      241 tatggagcag aaggacccaa tacatttgat tgttctggat ttacacagtg gtgttataaa
      301 caaataggca taagtatccc tagaacagtt gcaacacaaa gcaaggcagg tagtgcagta
      361 gatttaaaag ataagaccaa atggaaagca ggagacctat tgtgtagagt tggtggagga
      421 agcagtaatc acgtaatgat gtatattgga aatggtcaaa tgattcattc accacagact
      481 ggtgatgtag taaaaataca atcagttgat tcatatagaa aaggaaaagc atatacacat
      541 gtgagaagat ttatataagt gaggtgataa tatgagccaa gatttattac agataataaa
      601 aaaagctgca atggatgcag tagaaacaag caacccaatg agggttgtat ttggaacaat
      661 agaaagtact agccctctaa aagttaaaat agagcaaaaa ctatctattg gtgaattttt
      721 tctaatacaa acagatacat tcaaaaaata tacagataaa aaaataggag ataaattagt
      781 cttaattag
//
LOCUS       DAEMHZ010000285          780 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000285 DAEMHZ010000000
VERSION     DAEMHZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.290"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..658
                     /locus_tag="KPK72_003863"
     CDS             152..658
                     /locus_tag="KPK72_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG2490990.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          318 a           76 c          133 g          253 t
ORIGIN      
        1 aaaactactt attgcgaaag taattgattt gttgtattat aatttacatg aattatgtag
       61 gacaatcaaa agtcagtatt ataagagtac atatattcaa atttttaaca attaatttat
      121 ataattaaaa aatgaagggt aggatgaagt catgggtttt agagaagtaa aaattgagga
      181 attacagttt aatccattta ctaagattgg caaagaatgg ttacttataa cagctggtga
      241 ttcagaaaag tttaacacaa tgactgctag ctggggtggt gttggagtct attggggtaa
      301 aaatgttgtt acaacttaca taagaccaca gagatataca aaagaatttg tagatagtaa
      361 tgatacattt acagttgcct tctttgatga aacgtataga gaggctttaa atatttgtgg
      421 tactatttct ggtagagata taaataaaat agaaaaagca ggacttacac catattttgt
      481 ggatgataca gtagcttttg aagaagctaa tatgattata gtatgtaaaa agctatatca
      541 tggtaatatg ccaccagaaa actttgatgc taaagaaaat gataaaaaat ggtatccaga
      601 gaaagactac catactatgt acatttcaga aattataaaa gttcttgtaa aagaataatt
      661 ataataaaaa atatatgaaa agaacttatt ataagtaaat gactgtgctt tcttatctga
      721 tatctaagta gatgctagta actaaataaa tattaatttt aaataaatac tattataaaa
//
LOCUS       DAEMHZ010000286          664 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000286 DAEMHZ010000000
VERSION     DAEMHZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.291"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>664)
                     /locus_tag="KPK72_003864"
     rRNA            complement(<1..>664)
                     /locus_tag="KPK72_003864"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAEMHZ010000287          652 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000287 DAEMHZ010000000
VERSION     DAEMHZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.292"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            225..>652
                     /locus_tag="KPK72_003865"
     CDS             225..>652
                     /locus_tag="KPK72_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490991.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGV"
BASE COUNT          281 a           39 c          103 g          229 t
ORIGIN      
        1 ataccaaata aagtgtatat cttaaaataa cttactagag atacaaggat gctatgtgaa
       61 ttttgaagtg tgcagtttag ataataaata tatataaata ataaagaaaa tggatttata
      121 gtatttaaaa tttcttataa gggtaaaaaa tgctattaga aaatgtacta aaaatatttt
      181 tccaattctt tatagaaaaa taatgatatt taggagagat tgtaatgcaa attgatgtat
      241 tgataattga tggagataag aatagttctc aagagctgaa aaatttttta gaaggaaagg
      301 ggataaatgt ctatttagca tataattgta aagatgctat tataaaaata tttagcaaga
      361 aatttgactt aatattttta gagataattt tagttgatgg tgatggttgg gatttttgta
      421 aaaaaattag aaatataact acttgcccaa ttattcatat aacttatata aatgaaaatc
      481 aaagtatttt aaatgcttta aattctggag gagatgatta tttaataaag cctttaaatt
      541 tagaaatatt atatgcgaaa gtgaaagcta ttttaagaag aagagattta tttttaaata
      601 ataataaaaa taatataaaa ctagaagatt ataatagagc tagtggagta at
//
LOCUS       DAEMHZ010000288          640 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000288 DAEMHZ010000000
VERSION     DAEMHZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.293"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(14..>640)
                     /locus_tag="KPK72_003866"
     CDS             complement(14..>640)
                     /locus_tag="KPK72_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2490992.1"
                     /translation="FEYNIKEFENGGDLLKKYPNNVDIIFMDIDLNDENGFNVSKEIK
                     KFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYSLFKEAISSYLKECYKNEAILS
                     IKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFKSSMKEIESNLEEHNFFRCHKS
                     YLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLREKLNSCLVDVII"
BASE COUNT          219 a           94 c           45 g          282 t
ORIGIN      
        1 gcaaaaattt acctcaaatt attacatcta ccaaacaact atttaacttt tctctcaata
       61 attttatctt ataacgactt acaattattg aattttgatt tataaataca atattatttt
      121 ctatatctag catatttacc ttcattaaat ttataaggta gcttttatga catctaaaaa
      181 aattatgttc ttctagattt gattctattt ctttcatact actcttaaaa gtatatattc
      241 ctttttcagt atgtatagtt aaatcatttt ttaaaacttc taaatataaa atatcatcta
      301 cctttatgaa atatatcttg catttacatt ttatagataa aatagcttca tttttgtagc
      361 attcttttaa atatgatgat attgcttctt taaatagaga gtattttaag ggcttctcta
      421 aatatctata aactcctatc tcataacctt tctttatgta tttatttgat gaggatacaa
      481 atataattct tacactttca tcaaattttt tgatttcttt tgaaacatta aatccatttt
      541 catcatttaa gtctatatcc ataaatatga tatcaacatt attaggatac ttttttaata
      601 aatcgcctcc attttcaaac tctttgatat tgtattcaaa
//
LOCUS       DAEMHZ010000289          577 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000289 DAEMHZ010000000
VERSION     DAEMHZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.294"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>577
                     /locus_tag="KPK72_003867"
     rRNA            <1..>577
                     /locus_tag="KPK72_003867"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAEMHZ010000290          542 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000290 DAEMHZ010000000
VERSION     DAEMHZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.295"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          194 a           74 c           36 g          238 t
ORIGIN      
        1 gtataaattt agtatattat ttatattttc acttaaagca tctccttata tatttattct
       61 ttattctctt gtattcttta aacgttttat atctcctatt tttatctaaa attaagaaat
      121 attaagattt caagaactta tgttcgtaga aataggtatg aaaatcccct taatttataa
      181 ctctggtaaa tttattccag taaatactct atttaaaatt tctaagaatt actttgtaaa
      241 tatattatat agacttccaa aaattcctgc aacccacaaa aaaactctac acctcttcat
      301 tttagttttg attttaaata aactagtact atttaatact cgttaataaa cattaatgtt
      361 atataacgct aaaaatgact aaacatgatt ttctaaaaat aaatcatttt atttttgatt
      421 tttttcttac aattttttgc attattttta catataattt acaaattatt tggtcggtat
      481 ttatagacct ttaaaataaa ttttcttaat attcttaact atattctacc ataaatttcc
      541 at
//
LOCUS       DAEMHZ010000291          416 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000291 DAEMHZ010000000
VERSION     DAEMHZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.296"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            12..88
                     /locus_tag="KPK72_003868"
     tRNA            12..88
                     /locus_tag="KPK72_003868"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Pro,seq:tgg)
     gene            96..172
                     /locus_tag="KPK72_003869"
     tRNA            96..172
                     /locus_tag="KPK72_003869"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:His,seq:gtg)
     gene            181..256
                     /locus_tag="KPK72_003870"
     tRNA            181..256
                     /locus_tag="KPK72_003870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Lys,seq:ttt)
     gene            264..337
                     /locus_tag="KPK72_003871"
     tRNA            264..337
                     /locus_tag="KPK72_003871"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Cys,seq:gca)
     gene            344..415
                     /locus_tag="KPK72_003872"
     tRNA            344..415
                     /locus_tag="KPK72_003872"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:376..378,aa:Asn,seq:gtt)
BASE COUNT           93 a          100 c          120 g          103 t
ORIGIN      
        1 taatataaat tcgaggtgta gcgcagtttg gtagcgcaca tggtttggga ccatggggcc
       61 gggggttcga gtcccttcac ctcgaccaat ataaggtggg tatagctcag ttggttagag
      121 cgccagattg tggctctgga ggtcgtgagt tcgactctca ttatccaccc caattcatat
      181 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
      241 cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg gtaaggcagt ggactgcaac
      301 tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa tattccaagg tagctcaacg
      361 gtggagcaac cggctgttaa ccggtaggct gtgggttcga gccccaccct tggagc
//
LOCUS       DAEMHZ010000292          364 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000292 DAEMHZ010000000
VERSION     DAEMHZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.297"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KPK72_003873"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KPK72_003873"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="KPK72_003874"
     tRNA            291..362
                     /locus_tag="KPK72_003874"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAEMHZ010000293          358 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000293 DAEMHZ010000000
VERSION     DAEMHZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.denovo.298"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>358
                     /locus_tag="KPK72_003875"
     CDS             <1..>358
                     /locus_tag="KPK72_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG2490993.1"
                     /translation="HSGDSTTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFI
                     EFQNELYIIEVNPRASRTVPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKL
                     VSVKVPVFSMSKLSKVE"
BASE COUNT          146 a           41 c           65 g          106 t
ORIGIN      
        1 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
       61 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      121 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      181 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      241 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      301 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaag
//
LOCUS       DAEMHZ010000294        10600 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.guided.138, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000294 DAEMHZ010000000
VERSION     DAEMHZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.guided.138"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(537..686)
                     /locus_tag="KPK72_003876"
     CDS             complement(537..686)
                     /locus_tag="KPK72_003876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490994.1"
                     /translation="MTRVYYLFTTFDGILIRLNANLCKVSSKKKMSILLVDTGYIDIY
                     QLMKR"
     gene            complement(697..1512)
                     /locus_tag="KPK72_003877"
     CDS             complement(697..1512)
                     /locus_tag="KPK72_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBG2490995.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSTNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            1604..2872
                     /locus_tag="KPK72_003878"
                     /pseudo
     CDS             1604..2872
                     /locus_tag="KPK72_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2971..3591
                     /locus_tag="KPK72_003879"
     CDS             2971..3591
                     /locus_tag="KPK72_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2490996.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            3741..4145
                     /locus_tag="KPK72_003880"
                     /pseudo
     CDS             3741..4145
                     /locus_tag="KPK72_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            4270..5196
                     /locus_tag="KPK72_003881"
     CDS             4270..5196
                     /locus_tag="KPK72_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2490997.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEGNNTIPIHMATTTRVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            5210..5659
                     /locus_tag="KPK72_003882"
     CDS             5210..5659
                     /locus_tag="KPK72_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2490998.1"
                     /translation="MELIEWNYTYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTYINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRESSKVLEKCGFKIEEYCHNS"
     gene            6019..6426
                     /locus_tag="KPK72_003883"
     CDS             6019..6426
                     /locus_tag="KPK72_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2490999.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            6428..6658
                     /locus_tag="KPK72_003884"
     CDS             6428..6658
                     /locus_tag="KPK72_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2491000.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            7048..7251
                     /locus_tag="KPK72_003885"
     CDS             7048..7251
                     /locus_tag="KPK72_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2491001.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            7329..7682
                     /locus_tag="KPK72_003886"
                     /pseudo
     CDS             7329..7682
                     /locus_tag="KPK72_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            7754..8386
                     /locus_tag="KPK72_003887"
     CDS             7754..8386
                     /locus_tag="KPK72_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG2491002.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(8757..9626)
                     /locus_tag="KPK72_003888"
     CDS             complement(8757..9626)
                     /locus_tag="KPK72_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2491003.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(9723..10580)
                     /locus_tag="KPK72_003889"
     CDS             complement(9723..10580)
                     /locus_tag="KPK72_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2491004.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
BASE COUNT         3664 a         1636 c         1634 g         3666 t
ORIGIN      
        1 tttttacttg aacttttctt ttcatattct cctccataat cagaaatttt ataggacttt
       61 aaattctatt ttctgcacaa tttaattata cattaaaaat actatacatt ataaaaatac
      121 taatttaaaa ataattttta ttctaattct taaaacaatc ccacatattt tagaaaaggt
      181 actctataat atacaaacac ttctttttta cctaaattta ttttaacatc ctataatttt
      241 cataatattc tttaaatttt attcatataa acaatattat tctggatgct aatagaatta
      301 atctataaat catagtataa ttatgcagaa ataactcgaa tttcactact tatgaagaga
      361 ttgaatatta atgaatctaa atcatttgca ttacttaaga gttttgacta aagttgaaca
      421 ccatacaaaa gtagctactc aattgtttat accccaacca agcttaagta atgttatata
      481 gattctcgaa aaagaattta gtatcttttt atttgaaaaa cttatattaa atttgattat
      541 cttttcataa gttggtaaat gtcgatatat cctgtatcta caaggagtat cgacattttc
      601 tttttggaag atactttgca taagttggca tttaaccgta taagaatgcc atcaaaagta
      661 gtaaataagt agtaaactct agtcatacta ctaggattat aaccgttcca tttcagctct
      721 cgcagaacta aatgaaccat gtgcataata gccaagcgtc atagtaatgt ttttatgccc
      781 cataatatat tgcaatatat tagtactcat tcctttgtta gccatatttg tacagagtgt
      841 atgcctaaag gtatgtggcg taatctttgg tagcttgtcc tcatgggtct tgttatactt
      901 cttgactaac gctcttaaca tgctttgata atgagttgct tgttttggta atccatcttg
      961 tttaagaaat aggaagttct ggtaaccatc aacaatgatt ggttctgctt tagcgttctt
     1021 acgccgttca acgatacgtt caaatgattg ataagctctt tcagtcattg gaatttctcg
     1081 cttaccctgt gtggtctttg gaacttctac ataatagcca atcttagtat cactcaacaa
     1141 ttgatggtct accataatgg ctttattttt catatctaat tctaaagtca aaccgcataa
     1201 ttcagaaatg cgtagtcctg tttctaagag gataacaaca tcatcataat atttacaata
     1261 agtcttatcg ctctccataa aggataataa gcccatttcc tgttctggtg ttagagcgat
     1321 ttttggttct gttgtatctt ccaaaacatc acttaactga aaattaaatg gattctttct
     1381 aatacagtcg tcttgaattg ccatgaaaaa cgacgctttc agtgagcgct tataattgtc
     1441 aatggattta taagcacacc catcttcact cattcggata gcccagcttt tagcgtctga
     1501 taatttaacc atatcaatgc tttttgcacc tagtgggtca ttgatagtat ctatattttg
     1561 atttataatt tatacatttt tttaaaaaca gacttttaac tgtaaagaat ctatgtataa
     1621 acttagctat gttgtccgtg tatctacaaa ggatcttgac gagctaaaaa ggcgttgtga
     1681 tgaagtcaaa gacttttacg acgacttatc aatcaagctc gtgcgtccct ttggggatat
     1741 gttgggattg catggcgaat ttattcctgt cagcaaacgt tacatcaatg attatattca
     1801 atatgtcact tcggattttt tggctggact tggttttgga gcgacacaaa cccttggcga
     1861 acatgaggat atctatctta gctacaatct ggacactgga aagaatgtct atctaaaacc
     1921 tgcccttgct agtcaagggg taaagggttc tattaccaat gcgttagcgt ctgcgtttat
     1981 cggttcattg ggtggtggta aattattcag taacaatatg cttgtttact atgcggtact
     2041 attcggcgga caggcggtca tagttgaccc taaagcagaa cgcggcaaat ggaaagaaac
     2101 gctaccagaa atagcccatg agataaacat tgtcaattta accagtaatg atgaaaacaa
     2161 aggattgctt gacccttacg tcattttgag ccgtaaaaat gattctgaaa gtttagcgat
     2221 tgatatccta acattccttg ctggtatctc cagtcgtgac agtgataagt tcccagtgct
     2281 tagaaaagca atccgcaatg taacccaaag tgaacagcgg gggttattac ttgttattga
     2341 tgaattacga aaagaagata cttctatcag taacagcata gccgaccata tcgaaagttt
     2401 tgtagactat gattttgcac atctactgtt tagtgatgga aatgtgaagc agtctatcag
     2461 cttggagaaa cagctgaaca ttattcaagt tgctgacctc gtattgccag atgccgacac
     2521 tagttttgaa gaatatacaa caatagaact tcttagtgtt gctatgttga ttgttattag
     2581 tacctttgta ttagacttta ttcatactga ccgctctatc ttcaagatag ttgaccttga
     2641 cgaagcatgg tcttttttgc aggtggcaca aggtaaaacg ctatctatga aactggttag
     2701 agcatcgcaa gattcacagg acaaacgaat tgaacgtttg aagaattatc tgactgaaaa
     2761 cctacaacag ttgaatactg aaatggttcg ctcgtatatt cctactattt ctattgtcaa
     2821 cagctttccc agtttgagct tactttgcag aaaaatgaca actggaaaat aataatggaa
     2881 tagtttctat gtaaatatat ttttattatc ttacctaact aattgaaaga tgatataatt
     2941 taatcaaaga tttggtaagg agggatgaat atgcatttaa ttgattttat gaatgaagat
     3001 attaaaaaga agcttgttaa aaaaacgtat aaaaagaaag agactattct ttttgctgag
     3061 caagaaaatg aatatgtaat tcttatgatt gacggtattg cagaagcatt catcttaaat
     3121 gtgaaaggaa acatctcaac tattcatctt tatagaagtg gtagtttttt tggagaaatg
     3181 gaacaattta atgatgggaa aaaacccgtt gaaattgtcg ctttcacaga ttgtaatgta
     3241 tataaacttc atagagatga ttttttaaac tggttaaggg gagattttga agctacaaaa
     3301 tttctgattc gagaaatatc tagtaaatta gttgtaaatg ctgaattaat tgaagaatta
     3361 tcatctttaa gtgttaaaga acgacttctg aggtgtatat ccttacacta ttatagaagt
     3421 gaattaaatc tgcttaccaa aaaacagctt gctagcgaag taaacgctcc aattagaagc
     3481 attaatcgtg ctataaatga atgtgtacaa gaacatctaa ttgcttatga aaacaaaaga
     3541 tttagtgtaa ttaatcagcg agaggtatta aagaatcttc ctacaacata agtataattt
     3601 caaaaaagta ttgaataata attattcaat gcttttttat ttcgggtcaa gtgacctatt
     3661 tttttttatt tcttcattat ataatactcc tatgcataat tatgttggat tgatagctaa
     3721 taaaagaaag tgagaggtgt tatactaaaa aagaagcata cttcccaact ccagacttac
     3781 agtggattga agatagattt tggatttggg ttcattacgc ttgcactaag attggacgta
     3841 gagaactatt tgaaacaatt gattttttat cttttttaag acaatcagta cttgccccat
     3901 taatacagct tctcttaggt aaattacctc gtggagttag aaaaattgaa ttggacgcac
     3961 cccaatatat tgaagcatta gaaaagacgg ttgctaacca tgaatatgaa agctgtgtta
     4021 atgcattgca ggctactatc aacctgtatc ttgagttaag agaggaatta tcacaaagtc
     4081 ctattgtccg aaaggttgaa gctgaaagag ttgcattaca gtattttcaa gatttaatca
     4141 aataaaccca aagtaaaaaa atattgaata gtaaatattc agtgtttttt catttttggt
     4201 caaatgacct agattagttg ttgtaaccat tctataatta ttttgaaagt aacagtatgg
     4261 aggataacta tgagtgatat tttaagtaga attacaaata aaattgggga taagaatata
     4321 gttgataagt tatcagctct atcaaaatca gatttgaact cacttctatt agaagtgttt
     4381 gatagacaag caaatacttt aactgcaact gatattctaa agtcatacca actaaataga
     4441 tttactatcc ctagcggtat agatccagaa gaaattcatg ctttggaatc aaaattattg
     4501 agaaaagcat tcaatatgga tatcaagacg attatgcttt ctccctctgc cccattaggt
     4561 agctgtagcg tttttggctc cgtagatcaa tataacgtag taagtgctct acgagggaca
     4621 gaaacacttg cagacccctc aaatatgttg gcaataatta ttgctgataa attaaaaaga
     4681 aaagaaggaa ataataccat acctatccac atggctacta ctactagggt aataagagca
     4741 caaaattttg taggtaaagg tttatattca cattttggct tatattgtat ggtttcatct
     4801 ggtatagata cagggtctta cacttgtgaa aaaatgttgt tagaaaagca cttaaactat
     4861 tataaagata tattaagtga aatagaaaat gttcgtttct ctattattct acgcaaaaga
     4921 aacggataca aagataatga cggatttttc gacagaatgg ttgaaactat caaacttatt
     4981 tttcctaata tagagttatc aattcataat gatgatgttg ataataatta ttataaaggt
     5041 atcaatttca aattatcagt caaacaagga aatgaaaccg ttgaaatagt tgacggtgga
     5101 tttgttgact ggacttatca aatgcttgga aataaaaaag aacgctgttt gataagtggt
     5161 attggcttag aaagatttct tgtagctgtt tcataaaagg agaagaaaaa tggaacttat
     5221 agagtggaat tatacttata ttaccgattt agttgagttt gcgaatgata aagaaattgc
     5281 aaagaatctt cgtgacaact tcccccaccc ttatagggag caaaacgcta gagattttat
     5341 tagtttttgt ttggcaactc cagatgttaa acaacttagc tatgctattt tttatcaaga
     5401 aagggctatt ggaagtatca gcctaacatt tgaagaagat acctatatta ataaaaatgc
     5461 tgaattaggt tactggcttg ctaggaacta ttggggcaaa gggatagcaa caaatgcagt
     5521 aaaagaaata tgcaaaattg cctttgaaaa atacaacctt aacaaaattc atgctaatgt
     5581 atattcctac aatagagagt caagtaaagt attggaaaaa tgtggtttta agattgaaga
     5641 atactgtcac aattcataat aaagaataga aacatgattg attgccatgt atatagttta
     5701 attcgaccgc aatagcattt attagaatta acctactttt taataacaga ataagttttt
     5761 cccctctatt aatttcttat gcatctttgt atttttatga aaagctagcc aatacatatt
     5821 tccatacatc aatatgatgg tggcttacaa ccaatataaa ttaaatacgt tttaaagaat
     5881 acacattttt gccctatagg cagagtgtgt atttttttct cttttttttg aaatttatcg
     5941 aagatttaac gccctcccac tgtgatagga gtaagcaagg ttatttaact ccttgcgatg
     6001 gaaagagggt gaaatcttat ggttgatgaa aagacaattg aacatcaatt cgacagtttc
     6061 tgtaaaacag ttttgcgtaa ctatgctaga gatatttatg acgaaaataa acgcagaaat
     6121 gattatttgg tatctctcga atctttgtca cttgcagaat taagtaaatt gagtatttta
     6181 gatgattatg attctaacta catctgtatg gtttcttacg actacaacat acgaattgaa
     6241 gatgtattaa tggcacaagc aatcggaaaa cttacaaaaa gaaaacaaga tataattcta
     6301 ctttcattct ttctcaacat gacaaatgct gacatagcaa cattgatgga tttagcagaa
     6361 aataccatac attatcacaa aacaaatgca ctaaaagagt tgaaagaact catggaggaa
     6421 cattgaaatg aaacctacta aagaagtatt accttttaca attattttac ttgctgtaca
     6481 tggcgatgta aatgcaataa acggaatact taaacatttt gaacactata tcattcgtct
     6541 ttcacagaaa actttatttg atgaatacgg aaatccctat attcatgtag aagaagaaac
     6601 aaagcgaatg ctaggaacaa aactaatcac tgctattttg agatttaatt tgtcttaatc
     6661 tcgccctctc tccatgtatc aagctatttg gtacatggag aatcactaat tatttgtcta
     6721 taaaagccca caagctattg tgatttttta taggcaaatc aatgccgact gttctttgac
     6781 aactgaataa agtagataga tacgtttgtg atacagatag ccaacaaata ctatacgcca
     6841 tgaccttaat caagagagct actatatagt gatttgtgaa taacctttgg aacggttgtt
     6901 ttgccgtaac ctgtaccaca gtataatgat acacctgtat gacacggttc tgcccataag
     6961 aatgggattg gttgaaatac caatggagct taccaaagcc atctatctac ttctgaacta
     7021 cgaacataga aaggagtgat acattctatg aacaatactg atgtacctat ttgggaaaaa
     7081 tataccttga cgattgaaga agcgtcaaaa tactttcgta ttggagagaa aaaattacgt
     7141 aaacttgctg atgaaaacct tgattctggc tgggtcatta tgaatagcaa tcgcattcaa
     7201 ataaagagaa aacaatttga gaaaatcgtt gatacacttg acgttatcta atgaaattga
     7261 gccttatatg ttataatatt catatgacgt atcaagctca attcggttat tgagaggagc
     7321 ttcttatcat gtcaactgaa aaaagacgag ataataaaga tagaatttta agaactggag
     7381 agagccagag aaaagatgga agatacgcat ataagtatgt atattcattt ggtaaaccac
     7441 aatttgttta ctcttggaaa cttgtaccta ctgataaacc cccaaagtga aaacgtgatg
     7501 atatatcatt aagggataag gaaaaagcaa tacaaaatga ccttgatgat agcattgaca
     7561 ctattggtaa gaaaatgaca gtttgtcagt tatacgctaa aaagaatgta ctaagaaaga
     7621 atatccgact taacactaaa aaaggacgcc attatcttat gaagattctt aacaaggagt
     7681 gattctgtga agagatgtat tgtagtagtg gaatagtcaa aaacataata tatattaaag
     7741 gagagattta aatatgacta ttccaagttc aaataaggta tatccaagaa gtaatgatta
     7801 tcaaactgta tatcttaaaa atgtaataac aagggaaaat atagaagttg gagattacac
     7861 aatatataat gacttttata atgacccaag agattttgag aaaaacaatg tattgtatca
     7921 ttaccctata aacaatgata aattaataat tggtaagttt tgctcaatag catgtaatgc
     7981 aaaatttcta atgactagtg gaaatcactc aatgaaatca aaatccacat atacttttcc
     8041 catcttttat gaagaatgga aactcgatat caaccatata acggacgctt gggacaataa
     8101 gggtaacatt ataattggaa atgatgtgtg gataggttat gattctataa ttatgtcagg
     8161 tgtaaatatt ggtgatggtg caattattgg aacaagagca gtagttacta atgacgtccc
     8221 accgtattct attgttggag gagtacctgc aaaaattata aaaaagagat ttaataatga
     8281 tacaatttca aaattattag aaattaaatg gtgggattgg ccatataaaa aaattcaatc
     8341 aaacattaag catattcaat caggaaatat tgataaatta gtttaatatt ttataattta
     8401 tttatattaa actttacttt aataatcctt ttataagagc tttttcaaaa tttgtatatt
     8461 attttaatat atataaattt aatacaacat taaataaaat aaaaagtaaa gttacttaat
     8521 aaaaagttac tttacttttt attatttaaa ttcctgtttt tattataata ggtaaaattc
     8581 tattaaacta gttcacattt tcattattat ttatatatta tatatgaata taccattttg
     8641 ttttttaata aaaaaatcat gctacatcaa tagtaagcaa ctattaatgt agcatgattt
     8701 tttgtatcta aataatctca gtatttacct atcaatatag attgatatat caaaaactat
     8761 agtcttctat attcttctct ttttagcttt tggttactta gatagtaatc aatttcatca
     8821 attagacgtt gtttatcatc aggtaataca ccttttaaat tagcataatt tgatgcatga
     8881 ttacttctaa atacaatttt ttcattagca tctatattct ttatcatctc tttaatttct
     8941 tgtaacacag ccttatcctc tagtattgta aactctcctg ccttaagtaa atcgtaaagt
     9001 tcagtattag gttctaccat taaactaagt acacccacat aatctggtga aattgctcct
     9061 aacatttttc ctgtatcaac tgcatgctga tgtgtttttt tagttcctcc aagacctgct
     9121 attacagtgg ctgataattt aatcccactt tttttaactt ttctaccaac ttttattaat
     9181 tgttcactac taaaaccttt cttaatatct tctaatactt cattgcttcc actttcaagc
     9241 cctagataaa tcattttaac accaagtttt cttaattctt taagttcatc atcagatttt
     9301 ttctctatag atttaggaga gccgtacata gtaattcttt cacactctgg aaaaacttct
     9361 tttatatata aaataatatc tcttaatttc tccattggta ttatcaatgc atctccatct
     9421 gctaagaata ttctttctac atgcttataa tatcttctaa atatatcaat ttcatttttt
     9481 attacttcta gtggctttat tttaaatttt ttagatttgt acatactaca aaatgtacat
     9541 ttgttatgag aacatcctag tgtgacttgt attataagac tataagcctc acttggtggt
     9601 ctgtaaagtg gcatatcgta ttccatatta aaatccccct tgtaataaaa tattcattct
     9661 attatattat taatatttac tttaaataat aacttataaa attatatttt ttaaatttta
     9721 gtttaagata tattctttaa aaatttaatg atactattgg cagtaatatt aagttgtttt
     9781 tcttgactta taatagcatc attatctccc ttttgttttc catagtctcc aaattgtgca
     9841 tgatttcctc cttcaatctc aacataagtt gtatcatcag gaagtttttg ttttgattct
     9901 acaagacttt taaaattgac tactccatct ttgctccccc atatagatat aacttttttt
     9961 cctaattctt tcaactcatc acccattgga taagaagcta aaaatacaac gccttcaatt
    10021 aatttattat cacgtgcaaa atttgatgct gcaactcctc ctaatgaatg acctcctatt
    10081 acccaatgtt ctatgttgtt gtaagaatcc ataactttct gtgccttatt agttccaagt
    10141 atagctagat taagtggcat ctttacgatt acaacctcat atccactttc tgctattttt
    10201 cttgcaagtg gagcataaga ttctatttct actttagctc ctggatacaa aattaaacct
    10261 ttagaaactt ttcttccttt tggtgtaaat gaaatgtttt catttacagt tacttcaact
    10321 ttactattgc tgactagagc ttcttttgcc agcttttgag gttcatatgt tttatttatc
    10381 catatgaaaa agccaagcaa aatcaataca aatagtatac ctattccctt aaatatctta
    10441 ttctttatgt taaattttct attattattt ttatcatctt tgttctcaac ctcatcataa
    10501 agtaaaggtc tctcaagttc catagttata ctaaatgtat catatttctt attacgcttt
    10561 acctttacat ctttactcat aaatctaacc cccataatat
//
LOCUS       DAEMHZ010000295         4806 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.guided.206, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000295 DAEMHZ010000000
VERSION     DAEMHZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.guided.206"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            247..864
                     /locus_tag="KPK72_003890"
     CDS             247..864
                     /locus_tag="KPK72_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG2491005.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            972..3503
                     /locus_tag="KPK72_003891"
     CDS             972..3503
                     /locus_tag="KPK72_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2491006.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDVKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3937..4638
                     /locus_tag="KPK72_003892"
     CDS             3937..4638
                     /locus_tag="KPK72_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2491007.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
BASE COUNT         1903 a          446 c          897 g         1560 t
ORIGIN      
        1 tcaaaatgaa ctttttagtt catgaggcac tctttttgta cgaaataaaa tatattttta
       61 ttatttcaac aatgaaaaag aggcttgttt ccattttgag acagtataat ttttatgtaa
      121 ctttaatttt agtaaatata aagttttaaa attgataaaa taggaggaat ttttaatata
      181 aaagggtaaa taaagggttt agagggcata atatagaata aattaatata tttaaggagg
      241 gtttttatgt gtaaaaaaac gtatttatta gtggtaagta tgcttatagc gggtttatta
      301 actgcatgta gtgggagtcc aactaaggat tcaggacaag cacaagataa tacaaaaaag
      361 gaggcaaatg catcaaataa cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      421 caagatttta aagtgccata cacagatgca ataaacatat ttaaagataa atataaggat
      481 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      541 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      601 gaagataaaa cagaaaagtt ggattcagaa gatttaaatg gagttgctag aaaagaaaaa
      661 ttagatttaa atgacataat gacacctcaa caagctatgg aaattgcatt aaaagaacaa
      721 aatggaatag ttaaagaatg gagtttagac aaagatttag atgttacttt ttataaaata
      781 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      841 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      901 aataaatttt gtttaaaata taacaagcag ggtataaaga gactaaataa ctattttagg
      961 aggagtacgg tatgtcattg aaatcactaa aaataaaaga aaatttatat tgggtaggtt
     1021 cacttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1081 ataattcata tgtattaaaa ggaacagaaa aaacagtttt atttgagaca gttaaagata
     1141 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1201 actatattgt agtaagtcat actgaaccag accatgcagg aagtgttgaa aagcttttgg
     1261 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1321 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1381 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatact atatacacat
     1441 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1501 ataagatagt aaatacactt gatgaaaatg aagaaaagga ttacttggat gctttaagat
     1561 actattatga ttgtataatg ggtccattta aaccatctat ggtaactgct atagaaaaga
     1621 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1681 ctagaaaaat tatagacctt tattacaact ggagtgtaaa tgaacaaata aagttagaaa
     1741 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1801 taaaagcata cattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1861 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1921 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1981 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     2041 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2101 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2161 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2221 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2281 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2341 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2401 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2461 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttttac atggaagata
     2521 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2581 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2641 tagctacagg aagtagaaac tttataccac caattaaggg tcatgattta gaaaatgtgt
     2701 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2761 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2821 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2881 aagaaggtag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2941 gtgtagcagt tgatggtatt gaaggtgatg taaaagctca aaaagttgta tttaaagatg
     3001 gagatagtgt cgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3061 tggttcagga tacatctgta aaaattgata gaggtattgt tgtagataag acattacaga
     3121 caaatgttaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3181 caatatggcc atcttcagta gaaatgggaa aaatagcagg ggcaaatgca agtggagata
     3241 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3301 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3361 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3421 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3481 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtctcaaaa
     3541 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3601 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3661 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3721 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3781 cagtttttgt gttccaatgg tagcatcaac acattctgat gtagtagttc ttttcttata
     3841 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3901 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3961 gatgatgaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     4021 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa aaatttggat
     4081 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4141 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4201 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4261 gttgttgcaa gagtaaagac acaattaaga agatatatgc gatataataa ttcttatgaa
     4321 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4381 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4441 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4501 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4561 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4621 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4681 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4741 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4801 tcgtat
//
LOCUS       DAEMHZ010000296         3519 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.guided.232, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000296 DAEMHZ010000000
VERSION     DAEMHZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.guided.232"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            41..2179
                     /gene="vanT"
                     /locus_tag="KPK72_003893"
     CDS             41..2179
                     /gene="vanT"
                     /locus_tag="KPK72_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2491008.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            2493..3113
                     /locus_tag="KPK72_003894"
     CDS             2493..3113
                     /locus_tag="KPK72_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2491009.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(3279..>3519)
                     /locus_tag="KPK72_003895"
     CDS             complement(3279..>3519)
                     /locus_tag="KPK72_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2491010.1"
                     /translation="IFTNNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINI
                     SLLPIKTNSKTEKFISKLLNFIVIFLIGYQFYILL"
BASE COUNT         1264 a          389 c          568 g         1298 t
ORIGIN      
        1 tttggagaaa tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta
       61 tgcagggctt gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc
      121 tccattatta tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat
      181 tgctgtgcca ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa
      241 ttgtaataaa ctgaaatctt ttctaaaaaa tgtattattt atttacggaa tagctattgt
      301 aatttatatt cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa
      361 tattattaag gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc
      421 aattattggt gcaagtattg catactttct tgtaaaaaaa ctaggtttaa agtactcatt
      481 agtaatcact attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt
      541 agaaaaaata ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac
      601 acgaaatggt atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa
      661 atctaattat atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat
      721 gttatgtgag ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat
      781 tatgttattg ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg
      841 agctaggtta ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt
      901 tgttattcgt atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct
      961 tattcattat atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat
     1021 tcttaaacct ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg
     1081 gattgaaatt gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc
     1141 agaagattgt gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga
     1201 agtagcgact tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga
     1261 ggggattgat ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa
     1321 cccaataagg gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa
     1381 tcatgctatc tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac
     1441 aggtatgcac agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa
     1501 attaaaatac ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa
     1561 agatgaagat gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt
     1621 tctgttaaaa aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt
     1681 taattatccc aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt
     1741 aagttctcca aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa
     1801 atcacaagta attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt
     1861 ctttattgca agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat
     1921 acctagaaat ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat
     1981 agtgggtcga atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga
     2041 agttggtaat acggcaataa taataggaag agataattta tctgaattat ctgcttctga
     2101 agttgcaaat aactcatgta gtatatcaaa tgaattattg agcagagtag gaagaaggtt
     2161 aaatgttata aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt
     2221 ttactctgac tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca
     2281 tgacaattta agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt
     2341 ttgttataat atataataaa ataattatac cttaactgta aacttatata gattatttac
     2401 tttttaatat tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat
     2461 aatagttaaa gttaaaaaag gggaaaagca atatgcagta tatacaagaa ctacttgatt
     2521 tgactcaaaa ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa
     2581 gctttatacc agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat
     2641 tatttattgg atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct
     2701 tattaataag cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg
     2761 aaaaagcaat aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct
     2821 gtccatttat gcccggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa
     2881 aagattttgt gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg
     2941 tggcaagtga tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat
     3001 tggtgttttt gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt
     3061 atgatttcca tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac
     3121 gaaataaaac gtatcttaaa atgatttagt actaaattat tttaagatac gttttatttt
     3181 ttgtattaag gtacaaaatt tttagttctt ttgtaaacta aaatatataa aacttattta
     3241 tacttagttc aaaatattgt tatttatttt ttctacaact ataataaaat ataaaattga
     3301 tatccaatca aaaaaataac tatgaaattt aatagcttag atatgaattt ttctgttttt
     3361 gagttagttt ttataggtag taaagaaatg tttatggtaa tgggataaaa caattcaata
     3421 ccttctttag taataaggtc aagacacaag tgagatagat atcctattat aaacccactg
     3481 gacaaacatg agaaaactat attattgtta gtgaagatt
//
LOCUS       DAEMHZ010000297         3144 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.guided.238, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000297 DAEMHZ010000000
VERSION     DAEMHZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.guided.238"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..963
                     /locus_tag="KPK72_003896"
     CDS             <1..963
                     /locus_tag="KPK72_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2491011.1"
                     /translation="EYKAALNLYQQIFRNSMELFIILAMVIVFFIIFRVYLNWFTKYF
                     SEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVY
                     LAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFN
                     LSNISLEYSTVSLTRMLEQLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDN
                     LLRNAVYYSFEDTTIEITAVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNS
                     NSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL"
     gene            1224..2324
                     /gene="vanG"
                     /locus_tag="KPK72_003897"
     CDS             1224..2324
                     /gene="vanG"
                     /locus_tag="KPK72_003897"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG2491012.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            2321..3127
                     /locus_tag="KPK72_003898"
     CDS             2321..3127
                     /locus_tag="KPK72_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2491013.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
BASE COUNT         1195 a          356 c          570 g         1023 t
ORIGIN      
        1 gaatataaag ctgcattgaa tttatatcag cagattttcc gtaatagcat ggagctcttc
       61 ataatactag ctatggtgat tgtgtttttt ataatattcc gtgtttattt aaattggttt
      121 acaaaatatt ttagtgaaat aaatcaaggt attgactctc ttatcaaaga agatgttgga
      181 gaagtggctt tatctccaga gttattggca atagaaaaga aaataaactc aattaaacat
      241 atacttgaac aacgtaaatt tgagacacaa atggcagaac aacgtaagaa tgaacttatt
      301 gtttatcttg cacatgattt aaaaacacca cttacatcag ttatagggta tctgacacta
      361 cttcgagatg aaagtcagat ttctgaagaa ctgcgaaaaa aatatttatc tatttcgttg
      421 gataaggcag agcgtcttga agatttaatt aatgaatttt ttgaaattac aaggtttaat
      481 ttatcaaata tatcacttga atacagtaca gtaagcttaa ctcgtatgct tgaacaacta
      541 acatatgagt ttaaacctac tttgatgagt aagaatttaa aatatacact gaatgttaca
      601 ccaaatatga tgataaggtg tgatgttaat aaaatgcaac gtgtatttga taacttatta
      661 cgaaatgctg tttattatag ttttgaagat actactattg aaataacagc tgtacaggag
      721 gatgattata taaagcttaa atttataaat catagcaata cgattccgga ggaaaaactt
      781 gagcgtattt ttgaacaatt ttacaggttg gatacatctc gtaattcaaa cagtggtaga
      841 gctgggttgg gacttgctat agctaaagaa atagtggagc ttcataacgg gacaataaca
      901 gctcatagcg agaacaatat aattaccttt aaggttatta ttccgattgt aggaaatttg
      961 taagaattta agtataaaaa aataggaaat tctttaggat aaataaataa agagcactct
     1021 tgttattgat ataattactg tatcaaaaca agagtgcttt tattttgata cagtaattat
     1081 atcaatagaa aaatgtgaaa atatattatg tggaacagat gcaattgtaa gtgaaggttg
     1141 attggatttg aaatagaaaa agtattttaa catgtttctg tagattgtat aaaaataaat
     1201 aaatcaacaa aggagaatca aagatgaata ataaaaaaat tgcagttata tttggtggaa
     1261 actcaacaga atatgatgta tcattacaat cagcttttgc agtacttgag aatttgaata
     1321 cggaaaaata tgatattata ccaattggaa ttacaaggga gggtaattgg tatcattata
     1381 ttggagatta taataatatc cagaataaca cttggtttga aagggatact gatataattc
     1441 cagttattgt ttcgcagaac cgtgtcaaaa atggtattat tgaattttta attcctgatg
     1501 ctgtttctca gaatcatatt ccaaatagtg ctattgaatc atgtgaaaat gatattaaat
     1561 acactaaact tgattttgtg tttcctgtac tgcatggaaa aaatggagaa gatggaacag
     1621 tgcaggggtt atttgaattg gcaggtatac aggttattgg atgtggtact ctttcttcag
     1681 cattatgtat ggacaaagac aaatcacata aacttgttca ttcagaaggt attaaagtac
     1741 caaaatcaat attactaaaa tactcagata aatttaatgt ggctgaactg tcaaaagact
     1801 taacttatcc attatttata aaacctgtgc gtgcaggttc atcatttggt attagtaaaa
     1861 tttataaaaa agaagagtta caagtagcag tagacttggc ttttgaacat gataatgaag
     1921 taataattga ggaaaatatt gatggttttg aggtagggtg tgcagtttta ggaaacaatg
     1981 acttacttgt tggaagagta gatgaaatag aattgtcaaa aggatttttt gattatacag
     2041 aaaaatatac attgaaaagt tcaaaaattc atatgccagc tagaattgat ttggattgtg
     2101 agaaaagaat acaagagaca gcaaaaatta tttatagaac tcttggatgt tctggatttg
     2161 ctagagtaga tatgttttta actccatctg gtgaaattat ttttaatgaa gtcaatacaa
     2221 ttcctggctt tacttctcat agtcgttatc caaatatgat gaaaggtatt ggactgtctt
     2281 ttgaagaaat attggacaag ataattgagg tgtacataaa atgatgaata atattttatt
     2341 agataaaaat aaagaagata aaatgacaaa tactatctta ttgactaaag ataaagttta
     2401 tgatgggaat ctaattctag taaatgcttt tttaccagtt aaaacatctg aagatataga
     2461 tttaattcct gtagatacaa ggtttccaag tattcttatg aaacgtgaag ctacaaacat
     2521 tttacaaaat attttaaaaa gtatatgtgg aataaatgaa atagtacctg taagtggata
     2581 tcgtactgca gaggaacaac aagatatata ttcaagttct ctgcgtgaca atggaaaaga
     2641 ctttactaaa aagtttgtag cacttccaaa tcacagtgaa catcagacag ggcttgctat
     2701 tgatttaggg ttaaagaaag aagttataga ttttatctgc cctgaatttc cttataatgg
     2761 tatttgtaat gagtttcgta aggcagcact acattatggt tttatagagc gttatcagtg
     2821 taaaaaagaa gaaataacag gaatatctca agaaccatgg cattttcgtt atgtgggata
     2881 tccgcattct gagattatca ataaaatgaa tctcgctctt gaagaatata tagaatatat
     2941 taagagattt tcgtatacaa attgtttaaa aatagaagat aaagaaaaaa ttatagaaat
     3001 cttttatgtg cctattcata ctaaagaaga agtgagaatc tctatacctg aacaatgtgt
     3061 atatcaaata tcgggtaaca ataaggatgg ttttattgtg acattttgga gaaatagaaa
     3121 ggactaagaa atggaggaac aaga
//
LOCUS       DAEMHZ010000298         1082 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019459
            SAMN15907321-rid12310453.guided.278, whole genome shotgun sequence.
ACCESSION   DAEMHZ010000298 DAEMHZ010000000
VERSION     DAEMHZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907321
            Sequence Read Archive: SRR12520814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050666.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:41:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,899
            CDSs (total)                      :: 3,835
            Genes (coding)                    :: 3,757
            CDSs (with protein)               :: 3,757
            Genes (RNA)                       :: 64
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 19 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907321-rid12310453.guided.278"
                     /isolate="M2016019459"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-03"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..976
                     /gene="blaCDD"
                     /locus_tag="KPK72_003899"
     CDS             23..976
                     /gene="blaCDD"
                     /locus_tag="KPK72_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2491014.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYNVRE"
BASE COUNT          473 a           85 c          186 g          338 t
ORIGIN      
        1 aatatgggag atgatataaa ttatgaaaag aaaaaagaat tttatttgga tagctatctt
       61 attggttggt gtagttatgg taatgtatta tgtagggaaa aaacataacg atataaatca
      121 aaaaactgat aagaattata taaaatctga attaaataag tctgaattaa ataaatccaa
      181 aattgaaaac aataataaaa acaaaaaaga aaaaattaat atggttgact atagtgattg
      241 ttttgaaggt attagtggag gagctatttt ttataatact aaaaataaag aatataatat
      301 atataacaaa gaattaattg aaacaagaag gtcaccatgt tctactttta aaatagtttc
      361 aactttaatt ggtttagaaa aaggggtaat aaactcaaaa gaatcagtta tgggatatga
      421 tggtacagag tatccaaata aaaattggaa taaaaatctt agtttagaag aggcatttaa
      481 ggagtcttgt gtttggtact acaaaaaatt gatagataaa gttgatgcta aatctgtcca
      541 aaatatttta gatgatttaa agtatgggaa ttgcgatatt agcgaatggg aaggagattt
      601 aaaaaatgga aagggacatt taaatggatt ttggttagaa tcttcattac aaatatctcc
      661 aaaagaacaa gtgcaaacaa tggcaaaaat atttgaggga gatacaaact ttaaaaaaga
      721 gcatattaat attttaagag atattatgaa aatagatgta aatgataaaa acataaatgt
      781 atatggaaaa actggaacag gatttgatga aaaaaataaa cgtgtggatg catggtttgt
      841 tggtatgcta gagcgtgaag gtgacaccta ttattttgct attaaatcag atgattccaa
      901 taaagaaatt acagggccaa aggtaaagga aattgcaatt aatataatta agaaatatta
      961 taatgtaagg gaataaactt aattttattt gtaaatatta tttacctata ctctgtaata
     1021 tttattaaat aattttacta aattgaatct atatagagca aaactccttt agtacataaa
     1081 at
//
